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Sample records for bac library resource

  1. Library Resources for Bac End Sequencing. Final Technical Report

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    Pieter J. de Jong

    2000-10-01

    Studies directed towards the specific aims outlined for this research award are summarized. The RPCI II Human Bac Library has been expanded by the addition of 6.9-fold genomic coverage. This segment has been generated from a MBOI partial digest of the same anonymous donor DNA used for the rest of the library. A new cloning vector, pTARBAC1, has been constructed and used in the construction of RPCI-II segment 5. This new cloning vector provides a new strategy in identifying targeted genomic regions and will greatly facilitate a large-scale analysis for positional cloning. A new maleCS7BC/6J mouse BAC library has been constructed. RPCI-23 contain 576 plates (approx 210,000 clones) and represents approximately 11-fold coverage of the mouse genome.

  2. BAC library resources for map-based cloning and physical map construction in barley (Hordeum vulgare L.

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    Wu Cheng-Cang

    2011-05-01

    Full Text Available Background Although second generation sequencing (2GS technologies allow re-sequencing of previously gold-standard-sequenced genomes, whole genome shotgun sequencing and de novo assembly of large and complex eukaryotic genomes is still difficult. Availability of a genome-wide physical map is therefore still a prerequisite for whole genome sequencing for genomes like barley. To start such an endeavor, large insert genomic libraries, i.e. Bacterial Artificial Chromosome (BAC libraries, which are unbiased and representing deep haploid genome coverage, need to be ready in place. Result Five new BAC libraries were constructed for barley (Hordeum vulgare L. cultivar Morex. These libraries were constructed in different cloning sites (HindIII, EcoRI, MboI and BstXI of the respective vectors. In order to enhance unbiased genome representation and to minimize the number of gaps between BAC contigs, which are often due to uneven distribution of restriction sites, a mechanically sheared library was also generated. The new BAC libraries were fully characterized in depth by scrutinizing the major quality parameters such as average insert size, degree of contamination (plate wide, neighboring, and chloroplast, empty wells and off-scale clones (clones with 250 fragments. Additionally a set of gene-based probes were hybridized to high density BAC filters and showed that genome coverage of each library is between 2.4 and 6.6 X. Conclusion BAC libraries representing >20 haploid genomes are available as a new resource to the barley research community. Systematic utilization of these libraries in high-throughput BAC fingerprinting should allow developing a genome-wide physical map for the barley genome, which will be instrumental for map-based gene isolation and genome sequencing.

  3. Toward an Integrated BAC Library Resource for Genome Sequencing and Analysis

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    Simon, M. I.; Kim, U.-J.

    2002-02-26

    We developed a great deal of expertise in building large BAC libraries from a variety of DNA sources including humans, mice, corn, microorganisms, worms, and Arabidopsis. We greatly improved the technology for screening these libraries rapidly and for selecting appropriate BACs and mapping BACs to develop large overlapping contigs. We became involved in supplying BACs and BAC contigs to a variety of sequencing and mapping projects and we began to collaborate with Drs. Adams and Venter at TIGR and with Dr. Leroy Hood and his group at University of Washington to provide BACs for end sequencing and for mapping and sequencing of large fragments of chromosome 16. Together with Dr. Ian Dunham and his co-workers at the Sanger Center we completed the mapping and they completed the sequencing of the first human chromosome, chromosome 22. This was published in Nature in 1999 and our BAC contigs made a major contribution to this sequencing effort. Drs. Shizuya and Ding invented an automated highly accurate BAC mapping technique. We also developed long-term collaborations with Dr. Uli Weier at UCSF in the design of BAC probes for characterization of human tumors and specific chromosome deletions and breakpoints. Finally the contribution of our work to the human genome project has been recognized in the publication both by the international consortium and the NIH of a draft sequence of the human genome in Nature last year. Dr. Shizuya was acknowledged in the authorship of that landmark paper. Dr. Simon was also an author on the Venter/Adams Celera project sequencing the human genome that was published in Science last year.

  4. Development of genomic resources for Citrus clementina: Characterization of three deep-coverage BAC libraries and analysis of 46,000 BAC end sequences

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    Talon Manuel

    2008-09-01

    Full Text Available Abstract Background Citrus species constitute one of the major tree fruit crops of the subtropical regions with great economic importance. However, their peculiar reproductive characteristics, low genetic diversity and the long-term nature of tree breeding mostly impair citrus variety improvement. In woody plants, genomic science holds promise of improvements and in the Citrus genera the development of genomic tools may be crucial for further crop improvements. In this work we report the characterization of three BAC libraries from Clementine (Citrus clementina, one of the most relevant citrus fresh fruit market cultivars, and the analyses of 46.000 BAC end sequences. Clementine is a diploid plant with an estimated haploid genome size of 367 Mb and 2n = 18 chromosomes, which makes feasible the use of genomics tools to boost genetic improvement. Results Three genomic BAC libraries of Citrus clementina were constructed through EcoRI, MboI and HindIII digestions and 56,000 clones, representing an estimated genomic coverage of 19.5 haploid genome-equivalents, were picked. BAC end sequencing (BES of 28,000 clones produced 28.1 Mb of genomic sequence that allowed the identification of the repetitive fraction (12.5% of the genome and estimation of gene content (31,000 genes of this species. BES analyses identified 3,800 SSRs and 6,617 putative SNPs. Comparative genomic studies showed that citrus gene homology and microsyntheny with Populus trichocarpa was rather higher than with Arabidopsis thaliana, a species phylogenetically closer to citrus. Conclusion In this work, we report the characterization of three BAC libraries from C. clementina, and a new set of genomic resources that may be useful for isolation of genes underlying economically important traits, physical mapping and eventually crop improvement in Citrus species. In addition, BAC end sequencing has provided a first insight on the basic structure and organization of the citrus genome and has

  5. End Sequencing and Finger Printing of Human & Mouse BAC Libraries

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    Fraser, C

    2005-09-27

    This project provided for continued end sequencing of existing and new BAC libraries constructed to support human sequencing as well as to initiate BAC end sequencing from the mouse BAC libraries constructed to support mouse sequencing. The clones, the sequences, and the fingerprints are now an available resource for the community at large. Research and development of new metaodologies for BAC end sequencing have reduced costs and increase throughput.

  6. The 19 genomes of Drosophila: a BAC library resource for genus-wide and genome-scale comparative evolutionary research.

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    Song, Xiang; Goicoechea, Jose Luis; Ammiraju, Jetty S S; Luo, Meizhong; He, Ruifeng; Lin, Jinke; Lee, So-Jeong; Sisneros, Nicholas; Watts, Tom; Kudrna, David A; Golser, Wolfgang; Ashley, Elizabeth; Collura, Kristi; Braidotti, Michele; Yu, Yeisoo; Matzkin, Luciano M; McAllister, Bryant F; Markow, Therese Ann; Wing, Rod A

    2011-04-01

    The genus Drosophila has been the subject of intense comparative phylogenomics characterization to provide insights into genome evolution under diverse biological and ecological contexts and to functionally annotate the Drosophila melanogaster genome, a model system for animal and insect genetics. Recent sequencing of 11 additional Drosophila species from various divergence points of the genus is a first step in this direction. However, to fully reap the benefits of this resource, the Drosophila community is faced with two critical needs: i.e., the expansion of genomic resources from a much broader range of phylogenetic diversity and the development of additional resources to aid in finishing the existing draft genomes. To address these needs, we report the first synthesis of a comprehensive set of bacterial artificial chromosome (BAC) resources for 19 Drosophila species from all three subgenera. Ten libraries were derived from the exact source used to generate 10 of the 12 draft genomes, while the rest were generated from a strategically selected set of species on the basis of salient ecological and life history features and their phylogenetic positions. The majority of the new species have at least one sequenced reference genome for immediate comparative benefit. This 19-BAC library set was rigorously characterized and shown to have large insert sizes (125-168 kb), low nonrecombinant clone content (0.3-5.3%), and deep coverage (9.1-42.9×). Further, we demonstrated the utility of this BAC resource for generating physical maps of targeted loci, refining draft sequence assemblies and identifying potential genomic rearrangements across the phylogeny.

  7. New genomic resources for switchgrass: a BAC library and comparative analysis of homoeologous genomic regions harboring bioenergy traits

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    Feltus Frank A

    2011-07-01

    Full Text Available Abstract Background Switchgrass, a C4 species and a warm-season grass native to the prairies of North America, has been targeted for development into an herbaceous biomass fuel crop. Genetic improvement of switchgrass feedstock traits through marker-assisted breeding and biotechnology approaches calls for genomic tools development. Establishment of integrated physical and genetic maps for switchgrass will accelerate mapping of value added traits useful to breeding programs and to isolate important target genes using map based cloning. The reported polyploidy series in switchgrass ranges from diploid (2X = 18 to duodecaploid (12X = 108. Like in other large, repeat-rich plant genomes, this genomic complexity will hinder whole genome sequencing efforts. An extensive physical map providing enough information to resolve the homoeologous genomes would provide the necessary framework for accurate assembly of the switchgrass genome. Results A switchgrass BAC library constructed by partial digestion of nuclear DNA with EcoRI contains 147,456 clones covering the effective genome approximately 10 times based on a genome size of 3.2 Gigabases (~1.6 Gb effective. Restriction digestion and PFGE analysis of 234 randomly chosen BACs indicated that 95% of the clones contained inserts, ranging from 60 to 180 kb with an average of 120 kb. Comparative sequence analysis of two homoeologous genomic regions harboring orthologs of the rice OsBRI1 locus, a low-copy gene encoding a putative protein kinase and associated with biomass, revealed that orthologous clones from homoeologous chromosomes can be unambiguously distinguished from each other and correctly assembled to respective fingerprint contigs. Thus, the data obtained not only provide genomic resources for further analysis of switchgrass genome, but also improve efforts for an accurate genome sequencing strategy. Conclusions The construction of the first switchgrass BAC library and comparative analysis of

  8. Development of genomic resources for the prairie vole (Microtus ochrogaster: construction of a BAC library and vole-mouse comparative cytogenetic map

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    Young Larry J

    2010-01-01

    Full Text Available Abstract Background The prairie vole (Microtus ochrogaster is a premier animal model for understanding the genetic and neurological basis of social behaviors. Unlike other biomedical models, prairie voles display a rich repertoire of social behaviors including the formation of long-term pair bonds and biparental care. However, due to a lack of genomic resources for this species, studies have been limited to a handful of candidate genes. To provide a substrate for future development of genomic resources for this unique model organism, we report the construction and characterization of a bacterial artificial chromosome (BAC library from a single male prairie vole and a prairie vole-mouse (Mus musculus comparative cytogenetic map. Results We constructed a prairie vole BAC library (CHORI-232 consisting of 194,267 recombinant clones with an average insert size of 139 kb. Hybridization-based screening of the gridded library at 19 loci established that the library has an average depth of coverage of ~10×. To obtain a small-scale sampling of the prairie vole genome, we generated 3884 BAC end-sequences totaling ~2.8 Mb. One-third of these BAC-end sequences could be mapped to unique locations in the mouse genome, thereby anchoring 1003 prairie vole BAC clones to an orthologous position in the mouse genome. Fluorescence in situ hybridization (FISH mapping of 62 prairie vole clones with BAC-end sequences mapping to orthologous positions in the mouse genome was used to develop a first-generation genome-wide prairie vole-mouse comparative cytogenetic map. While conserved synteny was observed between this pair of rodent genomes, rearrangements between the prairie vole and mouse genomes were detected, including a minimum of five inversions and 16 inter-chromosomal rearrangements. Conclusions The construction of the prairie vole BAC library and the vole-mouse comparative cytogenetic map represent the first genome-wide modern genomic resources developed for this

  9. A HindIII BAC library construction of Mesobuthus martensii Karsch (Scorpiones:Buthidae): an important genetic resource for comparative genomics and phylogenetic analysis.

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    Li, Songryong; Ma, Yibao; Jang, Shenghun; Wu, Yingliang; Liu, Hui; Cao, Zhijian; Li, Wenxin

    2009-12-01

    Scorpions are "living but sophisticated fossils" that have changed little in their morphology since their first appearance over the past 450 million years ago. To provide a genetic resource for understanding the evolution of scorpion genome and the relationships between scorpions and other organisms, we first determined the genome size of the scorpion Mesobuthus martensii Karsch (about 600 Mbp) in the order Scorpiones and constructed a HindIII BAC library of the male scorpion M. martensii Karsch from China. The BAC library consists of a total of 46,080 clones with an average insert size of 100 kb, providing a 7.7-fold coverage of the scorpion haploid genome size of 600 Mbp as revealed in this study. High-density colony hybridization-based library screening was performed using 18S-5.8S-28S rRNA gene that is one of the most commonly used phylogenetic markers. Both library screening and PCR identification results revealed six positive BAC clones which were overlapped, and formed a contig of approximately 120 kb covering the rDNA. BAC DNA sequencing analysis determined the complete sequence of M. martensii Karsch rDNA unit that has a total length of 8779 bp, including 1813 bp 18s rDNA, 157 bp 5.8s rDNA, 3823 bp 28s rDNA, 530 bp ETS, 2168 bp ITS1 and 288 bp ITS2. Interestingly, some tandem repeats are present in the rRNA intergenic sequence (IGS) and ITS1/2 regions. These results demonstrated that the BAC library of the scorpion M. martensii Karsch and the complete sequence of rDNA unit will provide important genetic resources and tools for comparative genomics and phylogenetic analysis.

  10. Human BAC library: construction and rapid screening.

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    Asakawa, S; Abe, I; Kudoh, Y; Kishi, N; Wang, Y; Kubota, R; Kudoh, J; Kawasaki, K; Minoshima, S; Shimizu, N

    1997-05-20

    We have constructed a human genomic bacterial artificial chromosome (BAC) library using high molecular weight DNA from a pre-pro-B cell line, FLEB14-14, with a normal male diploid karyotype. This BAC library consists of 96,000 clones with an average DNA insert size of 110 kb, covering the human genome approximately 3 times. The library can be screened by three different methods. (1) Probe hybridization to 31 high-density replica (HDR) filters: each filter contains 3072 BAC clones which were gridded in a 6 x 6 pattern. (2) Probe hybridization to two Southern blot filters to which 31 HindIII digests of the pooled 3072 BAC clones were loaded. This identifies a particular HDR filter for which further probe hybridization is performed to identify a particular clone(s). (3) Two-step polymerase chain reaction (PCR). First, PCR is applied to DNA samples prepared from ten superpools of 9600 BAC clones each to identify a particular superpool and the second PCR is applied to 40 unique DNA samples prepared from the four-dimensionally assigned BAC clones of the particular superpool. We present typical examples of the library screening using these three methods. The two-step PCR screening is particularly powerful since it allows us to isolate a desired BAC clone(s) within a day or so. The theoretical consideration of the advantage of this method is presented. Furthermore, we have adapted Vectorette method to our BAC library for the isolation of terminal sequences of the BAC DNA insert to facilitate contig formation by BAC walking.

  11. Construction of genome-wide physical BAC contigs using mapped cDNA as probes: Toward an integrated BAC library resource for genome sequencing and analysis. Annual report, July 1995--January 1997

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    Mitchell, S.C.; Bocskai, D.; Cao, Y. [and others

    1997-12-31

    The goal of human genome project is to characterize and sequence entire genomes of human and several model organisms, thus providing complete sets of information on the entire structure of transcribed, regulatory and other functional regions for these organisms. In the past years, a number of useful genetic and physical markers on human and mouse genomes have been made available along with the advent of BAC library resources for these organisms. The advances in technology and resource development made it feasible to efficiently construct genome-wide physical BAC contigs for human and other genomes. Currently, over 30,000 mapped STSs and 27,000 mapped Unigenes are available for human genome mapping. ESTs and cDNAs are excellent resources for building contig maps for two reasons. Firstly, they exist in two alternative forms--as both sequence information for PCR primer pairs, and cDoreen genomic libraries efficiently for large number of DNA probes by combining over 100 cDNA probes in each hybridization. Second, the linkage and order of genes are rather conserved among human, mouse and other model organisms. Therefore, gene markers have advantages over random anonymous STSs in building maps for comparative genomic studies.

  12. Construction of the BAC Library of Small Abalone (Haliotis diversicolor) for Gene Screening and Genome Characterization.

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    Jiang, Likun; You, Weiwei; Zhang, Xiaojun; Xu, Jian; Jiang, Yanliang; Wang, Kai; Zhao, Zixia; Chen, Baohua; Zhao, Yunfeng; Mahboob, Shahid; Al-Ghanim, Khalid A; Ke, Caihuan; Xu, Peng

    2016-02-01

    The small abalone (Haliotis diversicolor) is one of the most important aquaculture species in East Asia. To facilitate gene cloning and characterization, genome analysis, and genetic breeding of it, we constructed a large-insert bacterial artificial chromosome (BAC) library, which is an important genetic tool for advanced genetics and genomics research. The small abalone BAC library includes 92,610 clones with an average insert size of 120 Kb, equivalent to approximately 7.6× of the small abalone genome. We set up three-dimensional pools and super pools of 18,432 BAC clones for target gene screening using PCR method. To assess the approach, we screened 12 target genes in these 18,432 BAC clones and identified 16 positive BAC clones. Eight positive BAC clones were then sequenced and assembled with the next generation sequencing platform. The assembled contigs representing these 8 BAC clones spanned 928 Kb of the small abalone genome, providing the first batch of genome sequences for genome evaluation and characterization. The average GC content of small abalone genome was estimated as 40.33%. A total of 21 protein-coding genes, including 7 target genes, were annotated into the 8 BACs, which proved the feasibility of PCR screening approach with three-dimensional pools in small abalone BAC library. One hundred fifty microsatellite loci were also identified from the sequences for marker development in the future. The BAC library and clone pools provided valuable resources and tools for genetic breeding and conservation of H. diversicolor.

  13. Two-dimensional screening of the Wageningen chicken BAC library.

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    Crooijmans, R P; Vrebalov, J; Dijkhof, R J; van der Poel, J J; Groenen, M A

    2000-05-01

    We have constructed a Bacterial Artificial Chromosome (BAC) library that provides 5.5-fold redundant coverage of the chicken genome. The library was made by cloning partial HindIII-digested high-molecular-weight (HMW) DNA of a female White Leghorn chicken into the HindIII site of the vector pECBAC1. Several modifications of standard protocols were necessary to clone efficiently large partial HindIII DNA fragments. The library consists of 49,920 clones arranged in 130 384-well plates. An average insert size of 134 kb was estimated from the analysis of 152 randomly selected BAC clones. The average number of NotI restriction sites per clone was 0.77. After individual growth, DNA was isolated of the pooled clones of each 384-well plate, and subsequently DNA of each plate was isolated from the individual row and column pools. Screening of the Wageningen chicken BAC library was performed by two-dimensional PCR with 125 microsatellite markers. For 124 markers at least one BAC clone was obtained. FISH experiments of 108 BAC clones revealed chimerism in less than 1%. The number of different BAC clones per marker present in the BAC library was examined for 35 markers which resulted in a total of 167 different BAC clones. Per marker the number of BAC clones varied from 1 to 11, with an average of 4.77. The chicken BAC library constitutes an invaluable tool for positional cloning and for comparative mapping studies.

  14. Construction of BAC libraries from flow-sorted chromosomes

    OpenAIRE

    Šafář, J.; Šimková, H; Doležel, J

    2016-01-01

    Cloned DNA libraries in bacterial artificial chromosome (BAC) are the most widely used form of large-insert DNA libraries. BAC libraries are typically represented by ordered clones derived from genomic DNA of a particular organism. In the case of large eukaryotic genomes, whole-genome libraries consist of a hundred thousand to a million clones, which make their handling and screening a daunting task. The labor and cost of working with whole-genome libraries can be greatly reduced by construct...

  15. Construction of BAC Libraries from Flow-Sorted Chromosomes.

    Science.gov (United States)

    Šafář, Jan; Šimková, Hana; Doležel, Jaroslav

    2016-01-01

    Cloned DNA libraries in bacterial artificial chromosome (BAC) are the most widely used form of large-insert DNA libraries. BAC libraries are typically represented by ordered clones derived from genomic DNA of a particular organism. In the case of large eukaryotic genomes, whole-genome libraries consist of a hundred thousand to a million clones, which make their handling and screening a daunting task. The labor and cost of working with whole-genome libraries can be greatly reduced by constructing a library derived from a smaller part of the genome. Here we describe construction of BAC libraries from mitotic chromosomes purified by flow cytometric sorting. Chromosome-specific BAC libraries facilitate positional gene cloning, physical mapping, and sequencing in complex plant genomes.

  16. Zebrafish YAC, BAC, and PAC genomic libraries.

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    Amemiya, C T; Zhong, T P; Silverman, G A; Fishman, M C; Zon, L I

    1999-01-01

    Numerous positional cloning projects directed at isolating genes responsible for the myriads of observed developmental defects in the zebrafish are anticipated in the very near future. In this chapter, we have reviewed the YAC, BAC, and PAC large-insert genomic resources available to the zebrafish community. We have discussed how these resources are screened and used in a positional cloning scheme and have pointed out frequently formidable logistical considerations in the approach. Despite being extremely tedious, positional cloning projects in the zebrafish will be comparatively easier to accomplish than in human and mouse, because of unique biological advantages of the zebrafish system. Moreover, the ease and speed at which genes are identified and cloned should rapidly increase as more mapping reagents and information become available, thereby paving the way for meaningful biological studies.

  17. Construction and characterization of a bovine BAC library with four genome-equivalent coverage

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    Eilertsen Ken

    2001-09-01

    Full Text Available Abstract A bovine artificial chromosome (BAC library of 105 984 clones has been constructed in the vector pBeloBAC11 and organized in 3-dimension pools and high density membranes for screening by PCR and hybridization. The average insert size, determined after analysis of 388 clones, was estimated at 120 kb corresponding to a four genome coverage. Given the fact that a male was used to construct the library, the probability of finding any given autosomal and X or Y locus is respectively 0.98 and 0.86. The library was screened for 164 microsatellite markers and an average of 3.9 superpools was positive for each PCR system. None of the 50 or so BAC clones analysed by FISH was chimeric. This BAC library increases the international genome coverage for cattle to around 28 genome equivalents and extends the coverage of the ruminant genomes available at the Inra resource center to 15 genome equivalents.

  18. Mining non-model genomic libraries for microsatellites: BAC versus EST libraries and the generation of allelic richness

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    Shaw Kerry L

    2010-07-01

    Full Text Available Abstract Background Simple sequence repeats (SSRs are tandemly repeated sequence motifs common in genomic nucleotide sequence that often harbor significant variation in repeat number. Frequently used as molecular markers, SSRs are increasingly identified via in silico approaches. Two common classes of genomic resources that can be mined are bacterial artificial chromosome (BAC libraries and expressed sequence tag (EST libraries. Results 288 SSR loci were screened in the rapidly radiating Hawaiian swordtail cricket genus Laupala. SSRs were more densely distributed and contained longer repeat structures in BAC library-derived sequence than in EST library-derived sequence, although neither repeat density nor length was exceptionally elevated despite the relatively large genome size of Laupala. A non-random distribution favoring AT-rich SSRs was observed. Allelic diversity of SSRs was positively correlated with repeat length and was generally higher in AT-rich repeat motifs. Conclusion The first large-scale survey of Orthopteran SSR allelic diversity is presented. Selection contributes more strongly to the size and density distributions of SSR loci derived from EST library sequence than from BAC library sequence, although all SSRs likely are subject to similar physical and structural constraints, such as slippage of DNA replication machinery, that may generate increased allelic diversity in AT-rich sequence motifs. Although in silico approaches work well for SSR locus identification in both EST and BAC libraries, BAC library sequence and AT-rich repeat motifs are generally superior SSR development resources for most applications.

  19. Isolation of BAC Clones Containing Conserved Genes from Libraries of Three Distantly Related Moths: A Useful Resource for Comparative Genomics of Lepidoptera

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    Yuji Yasukochi

    2011-01-01

    Full Text Available Lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests. To facilitate comparative genomics in Lepidoptera, we isolated BAC clones containing conserved and putative single-copy genes from libraries of three pests, Heliothis virescens, Ostrinia nubilalis, and Plutella xylostella, harboring the haploid chromosome number, =31, which are not closely related with each other or with the silkworm, Bombyx mori, (=28, the sequenced model lepidopteran. A total of 108–184 clones representing 101–182 conserved genes were isolated for each species. For 79 genes, clones were isolated from more than two species, which will be useful as common markers for analysis using fluorescence in situ hybridization (FISH, as well as for comparison of genome sequence among multiple species. The PCR-based clone isolation method presented here is applicable to species which lack a sequenced genome but have a significant collection of cDNA or EST sequences.

  20. Genome-wide BAC-end sequencing of Cucumis melo using two BAC libraries

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    Puigdomènech Pere

    2010-11-01

    Full Text Available Abstract Background Although melon (Cucumis melo L. is an economically important fruit crop, no genome-wide sequence information is openly available at the current time. We therefore sequenced BAC-ends representing a total of 33,024 clones, half of them from a previously described melon BAC library generated with restriction endonucleases and the remainder from a new random-shear BAC library. Results We generated a total of 47,140 high-quality BAC-end sequences (BES, 91.7% of which were paired-BES. Both libraries were assembled independently and then cross-assembled to obtain a final set of 33,372 non-redundant, high-quality sequences. These were grouped into 6,411 contigs (4.5 Mb and 26,961 non-assembled BES (14.4 Mb, representing ~4.2% of the melon genome. The sequences were used to screen genomic databases, identifying 7,198 simple sequence repeats (corresponding to one microsatellite every 2.6 kb and 2,484 additional repeats of which 95.9% represented transposable elements. The sequences were also used to screen expressed sequence tag (EST databases, revealing 11,372 BES that were homologous to ESTs. This suggests that ~30% of the melon genome consists of coding DNA. We observed regions of microsynteny between melon paired-BES and six other dicotyledonous plant genomes. Conclusion The analysis of nearly 50,000 BES from two complementary genomic libraries covered ~4.2% of the melon genome, providing insight into properties such as microsatellite and transposable element distribution, and the percentage of coding DNA. The observed synteny between melon paired-BES and six other plant genomes showed that useful comparative genomic data can be derived through large scale BAC-end sequencing by anchoring a small proportion of the melon genome to other sequenced genomes.

  1. Construction of a BAC library from cucumber (Cucumis sativus L.) and identification of linkage group specific clones

    Institute of Scientific and Technical Information of China (English)

    Yuan Guan; Qi Chen; Junsong Pan; Zheng Li; Huanle He; Aizhong Wu; Rentao Song; Run Cai

    2008-01-01

    A bacterial artificial chromosome (BAC) library consisting of 19,200 clones with an average insert size of 105 kb has been constructed from a cucumber (Cucumis sativus L.) inbred line S94, derived from a cultivar in North China. The entire library was equivalent to approximately 5 haploid cucumber genomes. To facilitate chromosome engineering and anchor the cucumber genetic linkage map to its chromosomes, 15 sequence-characterized amplified regions (SCAR) and seven simple sequence repeats (SSR) markers from each link-age group of cucumber were used to screen an ordered array of pooled BAC DNA with polymerase chain reaction (PCR). Fifteen mark-ers gave at least two positive clones. As a result, 22 BAC clones representing 7 linkage groups of cucumber were identified, which further validated the genome coverage and utility of the library. This BAC library and linkage group specific clones provide essential resources for future research of the cucumber genome.

  2. Advancing Eucalyptus genomics: identification and sequencing of lignin biosynthesis genes from deep-coverage BAC libraries

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    Kudrna David

    2011-03-01

    Full Text Available Abstract Background Eucalyptus species are among the most planted hardwoods in the world because of their rapid growth, adaptability and valuable wood properties. The development and integration of genomic resources into breeding practice will be increasingly important in the decades to come. Bacterial artificial chromosome (BAC libraries are key genomic tools that enable positional cloning of important traits, synteny evaluation, and the development of genome framework physical maps for genetic linkage and genome sequencing. Results We describe the construction and characterization of two deep-coverage BAC libraries EG_Ba and EG_Bb obtained from nuclear DNA fragments of E. grandis (clone BRASUZ1 digested with HindIII and BstYI, respectively. Genome coverages of 17 and 15 haploid genome equivalents were estimated for EG_Ba and EG_Bb, respectively. Both libraries contained large inserts, with average sizes ranging from 135 Kb (Eg_Bb to 157 Kb (Eg_Ba, very low extra-nuclear genome contamination providing a probability of finding a single copy gene ≥ 99.99%. Libraries were screened for the presence of several genes of interest via hybridizations to high-density BAC filters followed by PCR validation. Five selected BAC clones were sequenced and assembled using the Roche GS FLX technology providing the whole sequence of the E. grandis chloroplast genome, and complete genomic sequences of important lignin biosynthesis genes. Conclusions The two E. grandis BAC libraries described in this study represent an important milestone for the advancement of Eucalyptus genomics and forest tree research. These BAC resources have a highly redundant genome coverage (> 15×, contain large average inserts and have a very low percentage of clones with organellar DNA or empty vectors. These publicly available BAC libraries are thus suitable for a broad range of applications in genetic and genomic research in Eucalyptus and possibly in related species of Myrtaceae

  3. A highly redundant BAC library of Atlantic salmon (Salmo salar: an important tool for salmon projects

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    Koop Ben F

    2005-04-01

    library. We have demonstrated the library's ability to identify specific genes and genetic markers using hybridization, PCR and fingerprinting experiments. In addition, multiple fingerprinting contigs indicated a pseudo-tetraploidity of the Atlantic salmon genome. The highly redundant CHORI-214 BAC library is expected to be an important resource for mapping and sequencing of the Atlantic salmon genome.

  4. Preparation of high molecular weight gDNA and bacterial artificial chromosome (BAC) libraries in plants.

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    Biradar, Siddanagouda S; Nie, Xiaojun; Feng, Kewei; Weining, Song

    2014-01-01

    Bacterial artificial chromosome (BAC) libraries are extremely valuable large-insert DNA libraries for physical mapping, positional cloning, comparative genomic analysis, complete genome sequencing, and evolutionary studies. Due to their stability and relative simplicity BAC libraries are most preferred over other approaches for cloning large genomic DNA fragments for large-insert libraries. Isolation of intact high molecular weight (HMW) DNA is a critical step underlying the success of large-insert genomic DNA library construction. It requires the isolation of purified nuclei, embedding them into LMP agarose plugs, restriction digestion of the plugs, and quite often size selection using PFGE and electro-elution of insert DNA. The construction of BAC libraries is complex and challenging for most molecular laboratories. To facilitate the construction of BAC libraries, we present a step-by-step protocol for isolation of HMW DNA and construction of plant BAC libraries.

  5. Construction of BIBAC and BAC libraries from a variety of organisms for advanced genomics research.

    Science.gov (United States)

    Zhang, Hong-Bin; Scheuring, Chantel F; Zhang, Meiping; Zhang, Yang; Wu, Cheng-Cang; Dong, Jennifer J; Li, Yaning

    2012-02-16

    Large-insert BAC (bacterial artificial chromosome) and BIBAC (binary BAC) libraries are essential for modern genomics research for all organisms. We helped pioneer the BAC and BIBAC technologies, and by using them we have constructed hundreds of BAC and BIBAC libraries for different species of plants, animals, marine animals, insects, algae and microbes. These libraries have been used globally for different aspects of genomics research. Here we describe the procedure with the latest improvements that we have made and used for construction of BIBAC libraries. The procedure includes the preparation of BIBAC vectors, the preparation of clonable fragments of the desired size from the source DNA, the construction and transformation of BIBACs and, finally, the characterization and assembly of BIBAC libraries. We also specify the modifications necessary for construction of BAC libraries using the protocol. The entire protocol takes ∼7 d.

  6. Preliminary Study of BAC Library Construction in Black Tiger Shrimp, Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Suwit WUTHISUTHIMETHAVEE

    2009-01-01

    Full Text Available Availability of shrimp genome information is necessary for shrimp genetic studies and large-insert DNA clones, bacterial artificial chromosome (BACs serve as valuable tools for obtaining genomic sequences. The construction of a BAC library was achieved from this preliminary study of P. monodon. High molecular weight (HMW genomic DNA was isolated from abdominal muscle and the resulting hemocytes were of high quality and sufficient quantity for a BAC library construction. This BAC library was constructed from hemocyte HMW genomic DNA fragments ranging between 100 and 300 kb ligated to a pBAC-lac vector. The average insert size of the BAC clones was calculated as 100 kb ranging from 40 - 150 kb with over 50 % of the clones containing inserts larger than 100 kb. This BAC library contained approximately 60,000 BAC clones representing a 3-fold coverage of the haploid shrimp genome size. Furthermore, the consistency of BAC clones was also proven as no apparent rearrangements were observed before and after 100 generations in serial growth. From the above result, it is essential to improve the insert size and clone numbers of the BAC library to be a powerful tool in the future.

  7. Construction and sequence sampling of deep-coverage, large-insert BAC libraries for three model lepidopteran species

    Directory of Open Access Journals (Sweden)

    Zhao Shaying

    2009-06-01

    Full Text Available Abstract Background Manduca sexta, Heliothis virescens, and Heliconius erato represent three widely-used insect model species for genomic and fundamental studies in Lepidoptera. Large-insert BAC libraries of these insects are critical resources for many molecular studies, including physical mapping and genome sequencing, but not available to date. Results We report the construction and characterization of six large-insert BAC libraries for the three species and sampling sequence analysis of the genomes. The six BAC libraries were constructed with two restriction enzymes, two libraries for each species, and each has an average clone insert size ranging from 152–175 kb. We estimated that the genome coverage of each library ranged from 6–9 ×, with the two combined libraries of each species being equivalent to 13.0–16.3 × haploid genomes. The genome coverage, quality and utility of the libraries were further confirmed by library screening using 6~8 putative single-copy probes. To provide a first glimpse into these genomes, we sequenced and analyzed the BAC ends of ~200 clones randomly selected from the libraries of each species. The data revealed that the genomes are AT-rich, contain relatively small fractions of repeat elements with a majority belonging to the category of low complexity repeats, and are more abundant in retro-elements than DNA transposons. Among the species, the H. erato genome is somewhat more abundant in repeat elements and simple repeats than those of M. sexta and H. virescens. The BLAST analysis of the BAC end sequences suggested that the evolution of the three genomes is widely varied, with the genome of H. virescens being the most conserved as a typical lepidopteran, whereas both genomes of H. erato and M. sexta appear to have evolved significantly, resulting in a higher level of species- or evolutionary lineage-specific sequences. Conclusion The high-quality and large-insert BAC libraries of the insects, together

  8. A Blumeria graminis f.sp. hordei BAC library - contig building and microsynteny studies

    DEFF Research Database (Denmark)

    Pedersen, C.; Wu, B.; Giese, H.

    2002-01-01

    A bacterial artificial chromosome (BAC) library of Blumeria graminis f.sp. hordei, containing 12,000 clones with an average insert size of 41 kb, was constructed. The library represents about three genome equivalents and BAC-end sequencing showed a high content of repetitive sequences, making...... contigs, at or close to avirulence loci, were constructed. Single nucleotide polymorphism (SNP) markers were developed from BAC-end sequences to link the contigs to the genetic maps. Two other BAC contigs were used to study microsynteny between B. graminis and two other ascomycetes, Neurospora crassa...

  9. Construction and characterization of two Citrus BAC libraries and identification of clones containing the phytoene synthase gene.

    Science.gov (United States)

    Baig, M N R; Yu, An; Guo, Wenwu; Deng, Xiuxin

    2009-05-01

    Two deep-coverage Bacterial Artificial Chromosome (BAC) libraries of Citrus sinensis (L.) Osbeck 'Cara Cara' navel orange and Citrus reticulata (L.) Blanco 'Egan No. 1' Ponkan mandarin, which belong to the two most important species of the Citrus genus, have been constructed and characterized to facilitate gene cloning and to analyze variety-specific genome composition. The C. sinensis BAC library consists of 36 000 clones with negligible false-positive clones and an estimated average insert size of 126 kb covering ~4.5 x 109 bp and thus providing an 11.8-fold coverage of haploid genome equivalents, whereas the C. reticulata library consists of 21 000 clones also with negligible false-positive clones and an estimated average of 120 kb covering ~2.5 x 109 bp representing a 6.6-fold coverage of haploid genome equivalents. Both libraries were evaluated for contamination with high-copy vector, empty pIndigoBAC536 vector, and organellar DNA sequences. Screening has been performed by Southern hybridization of BAC filters, which results in genomics research in the two important species C. sinensis and C. reticulata. Resources, high-density filters, individual clones, and whole libraries are available for public distribution and are accessible at the National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University.

  10. [Three-dimensional PCR-based screening of Chinese fine wool merino sheep BAC library].

    Science.gov (United States)

    Wu, Xindong; Chen, Fang; Li, Xin; Zou, Yihui; Qiu, Wei; Gao, Jianfeng

    2008-10-01

    For rapid screening, we constructed two levels pools (primary and secondary pools) of the bacterial artificial chromosome (BAC) library of Chinese fine wool merino sheep. The primary pools were based on the individual 384-well microtiter plate and were prepared with a three-dimensional pooling scheme. Three dimension (plate, row and column) pools were made for each. The secondary pools were based on the entire BAC library. We developed a PCR based strategy to identify positive BACs from sheep BAC library. First, we analyzed secondary pools DNAs, according to the result, we analyzed correlative primary pools. It was one-step screening (66 PCR reactions) that we could screen a single positive clone from 74 000 BACs by our method, or three-step screening (less than 100 PCR reactions) could screen more clones. By one-step screening (66 PCR reactions), we screened successfully a positive clone 373D13 with polymorphism marker BF94-1.

  11. Gram negative shuttle BAC vector for heterologous expression of metagenomic libraries.

    Science.gov (United States)

    Kakirde, Kavita S; Wild, Jadwiga; Godiska, Ronald; Mead, David A; Wiggins, Andrew G; Goodman, Robert M; Szybalski, Waclaw; Liles, Mark R

    2011-04-15

    Bacterial artificial chromosome (BAC) vectors enable stable cloning of large DNA fragments from single genomes or microbial assemblages. A novel shuttle BAC vector was constructed that permits replication of BAC clones in diverse Gram-negative species. The "Gram-negative shuttle BAC" vector (pGNS-BAC) uses the F replicon for stable single-copy replication in E. coli and the broad-host-range RK2 mini-replicon for high-copy replication in diverse Gram-negative bacteria. As with other BAC vectors containing the oriV origin, this vector is capable of an arabinose-inducible increase in plasmid copy number. Resistance to both gentamicin and chloramphenicol is encoded on pGNS-BAC, permitting selection for the plasmid in diverse bacterial species. The oriT from an IncP plasmid was cloned into pGNS-BAC to enable conjugal transfer, thereby allowing both electroporation and conjugation of pGNS-BAC DNA into bacterial hosts. A soil metagenomic library was constructed in pGNS-BAC-1 (the first version of the vector, lacking gentamicin resistance and oriT), and recombinant clones were demonstrated to replicate in diverse Gram-negative hosts, including Escherichia coli, Pseudomonas spp., Salmonella enterica, Serratia marcescens, Vibrio vulnificus and Enterobacter nimipressuralis. This shuttle BAC vector can be utilized to clone genomic DNA from diverse sources, and then transfer it into diverse Gram-negative bacterial species to facilitate heterologous expression of recombinant pathways.

  12. Physical Analysis of the Complex Rye (Secale cereale L.) Alt4 Aluminium (Aluminum) Tolerance Locus Using a Whole-Genome BAC Library of Rye cv. Blanco

    Science.gov (United States)

    Rye is a diploid crop species with many outstanding qualities, and is also important as a source of new traits for wheat and triticale improvement. Here we describe a BAC library of rye cv. Blanco, representing a valuable resource for rye molecular genetic studies. The library provides a 6 × genome ...

  13. Construction of a quinoa (Chenopodium quinoa Willd.) BAC library and its use in identifying genes encoding seed storage proteins.

    Science.gov (United States)

    Stevens, M R; Coleman, C E; Parkinson, S E; Maughan, P J; Zhang, H-B; Balzotti, M R; Kooyman, D L; Arumuganathan, K; Bonifacio, A; Fairbanks, D J; Jellen, E N; Stevens, J J

    2006-05-01

    Quinoa (Chenopodium quinoa Willd.) is adapted to the harsh environments of the Andean Altiplano region. Its seeds have a well-balanced amino acid composition and exceptionally high protein content with respect to human nutrition. Quinoa grain is a staple in the diet of some of the most impoverished people in the world. The plant is an allotetraploid displaying disomic inheritance (2n=4x=36) with a di-haploid genome of 967 Mbp (megabase pair), or 2C=2.01 pg. We constructed two quinoa BAC libraries using BamHI (26,880 clones) and EcoRI (48,000 clones) restriction endonucleases. Cloned inserts in the BamHI library average 113 kb (kilobase) with approximately 2% of the clones lacking inserts, whereas cloned inserts in the EcoRI library average 130 kb and approximately 1% lack inserts. Three plastid genes used as probes of high-density arrayed blots of 73,728 BACs identified approximately 2.8% of the clones as containing plastid DNA inserts. We estimate that the combined quinoa libraries represent at least 9.0 di-haploid nuclear genome equivalents. An average of 12.2 positive clones per probe were identified with 13 quinoa single-copy ESTs as probes of the high-density arrayed blots, suggesting that the estimate of 9.0x coverage of the genome is conservative. Utility of the BAC libraries for gene identification was demonstrated by probing the library with a partial sequence of the 11S globulin seed storage protein gene and identifying multiple positive clones. The presence of the 11S globulin gene in four of the clones was verified by direct comparison with quinoa genomic DNA on a Southern blot. Besides serving as a useful tool for gene identification, the quinoa BAC libraries will be an important resource for physical mapping of the quinoa genome.

  14. Korean BAC library construction and characterization of HLA-DRA, HLA-DRB3.

    Science.gov (United States)

    Park, Mi-Hyun; Lee, Hye-Ja; Bok, Jeong; Kim, Cheol-Hwan; Hong, Seong-Tshool; Park, Chan; Kimm, KuChan; Oh, Bermseok; Lee, Jong-Young

    2006-07-31

    A human bacterial artificial chromosome (BAC) library was constructed with high molecular weight DNA extracted from the blood of a male Korean. This Korean BAC library contains 100,224 clones of insert size ranging from 70 to 150 kb, with an average size of 86 kb, corresponding to a 2.9-fold redundancy of the genome. The average insert size was determined from 288 randomly selected BAC clones that were well distributed among all the chromosomes. We developed a pooling system and three-step PCR screen for the Korean BAC library to isolate desired BAC clones, and we confirmed its utility using primer pairs designed for one of the clones. The Korean BAC library and screening pools will allow PCR-based screening of the Korean genome for any gene of interest. We also determined the allele types of HLA-DRA and HLA-DRB3 of clone KB55453, located in the HLA class II region on chromosome 6p21.3. The HLA-DRA and DRB3 genes in this clone were identified as the DRA*010202 and DRB3*01010201 types, respectively. The haplotype found in this library will provide useful information in future human disease studies.

  15. Gene Cloning of Penicillin V Acylase from Bacillus sp BAC4 by Genomic Library

    Directory of Open Access Journals (Sweden)

    ELFI SUSANTI VH

    2004-01-01

    Full Text Available This research was aimed to clone and identify penicillin V acylase (PVA gene of Bacillus sp. BAC4 by genomic library. Chromosome DNA of Bacillus sp. BAC4 was isolated by Wang method. pHB201 of E. coli was isolated by alkali lyses method. Recombinant DNA of Bacillus sp. BAC4 chromosome fragment and pHB201 was made by ligase process using T4 DNA ligase. Transformation of E. coli using this recombinant plasmid was carried out according to Mandel-Higa method. The results indicated that chromosome DNA fragment of Bacillus sp. BAC4 was bigger 23 kb with purity 1,3. Plasmid DNA fragment of E coli was 6,5 kb. Transformants laboring pHB201 recombinant plasmid was screen as blue-white colonies in a medium containing IPTG/X-gal and chloramphenicol.

  16. Library resources on the Internet

    Science.gov (United States)

    Buchanan, Nancy L.

    1995-07-01

    Library resources are prevalent on the Internet. Library catalogs, electronic books, electronic periodicals, periodical indexes, reference sources, and U.S. Government documents are available by telnet, Gopher, World Wide Web, and FTP. Comparatively few copyrighted library resources are available freely on the Internet. Internet implementations of library resources can add useful features, such as full-text searching. There are discussion lists, Gophers, and World Wide Web pages to help users keep up with new resources and changes to existing ones. The future will bring more library resources, more types of library resources, and more integrated implementations of such resources to the Internet.

  17. BAC Library Construction and Physical Mapping of Bacillus anthracis A16R

    Institute of Scientific and Technical Information of China (English)

    Zhang Da; Zhu Houchu; Huang Liuyu

    2013-01-01

    Bacillus anthracis is an endospore-forming bacterium that causes severe inhalational anthrax, and bacillus anthracis A16R is an attenuated strain derived from Bacillus anthracis A16. The development of bacterial artificial chromosome (BAC) system has allowed the construction of large insert-size DNA libraries, and the bacterial artificial chromosomes (BACs) have become the preferred large insert cloning system for genomic analysis because such libraries are characteristically stable, high in ifdelity and easy to handle. To facilitate genome studies of this bacterium, a bacterial artiifcial chromosome library (BAC) has been established from genome DNA of Bacillus anthracis A16R. This library consisted of 9 600 clones randomly selected from more than 15 000 recombinant clones carrying inserts in the plindigoBAC-5 vectors. The mean insert size was 56 kbp, representing an approximate 12-fold genome coverage, while end sequences were obtained from 700 randomly selected clones. Sequences were compared with Bacillus anthracis Ames and Bacillus cereus ATCC 14579 Genome Project databases using the NCBI BLASTN search project. And most BLASTN results showed high identities and that the sequences’ sites could be used as STSs. To construct this physical map, Excel was used for the array of STSs and some gaps of the map were iflled up by PCR walking. Artemis-V4 was used in the construction of a genome-wide physical map with 93%genome coverage. The A16R BAC library proved to be a vital tool for the generation of a map that would not only allow the subsequent sequencing of defined areas of genome, but also provide immediate access to clones that were stable and convenient for functional genomic researches.

  18. BAC Library Construction and Physical Mapping of Bacillus anthracis A16R

    Directory of Open Access Journals (Sweden)

    Da Zhang

    2013-12-01

    Full Text Available Bacillus anthracis is an endospore-forming bacterium that causes severe inhalational anthrax, and bacillus anthracis A16R is an attenuated strain derived from Bacillus anthracis A16. The development of bacterial artificial chromosome (BAC system has allowed the construction of large insert-size DNA libraries, and the bacterial artificial chromosomes (BACs have become the preferred large insert cloning system for genomic analysis because such libraries are characteristically stable, high in fidelity and easy to handle. To facilitate genome studies of this bacterium, a bacterial artificial chromosome library (BAC has been established from genome DNA of Bacillus anthracis A16R. This library consisted of 9 600 clones randomly selected from more than 15 000 recombinant clones carrying inserts in the plindigoBAC-5 vectors. The mean insert size was 56 kbp, representing an approximate 12-fold genome coverage, while end sequences were obtained from 700 randomly selected clones. Sequences were compared with Bacillus anthracis Ames and Bacillus cereus ATCC 14579 Genome Project databases using the NCBI BLASTN search project. And most BLASTN results showed high identities and that the sequences’ sites could be used as STSs. To construct this physical map, Excel was used for the array of STSs and some gaps of the map were filled up by PCR walking. Artemis-V4 was used in the construction of a genome-wide physical map with 93% genome coverage. The A16R BAC library proved to be a vital tool for the generation of a map that would not only allow the subsequent sequencing of defined areas of genome, but also provide immediate access to clones that were stable and convenient for functional genomic researches.

  19. Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener

    Directory of Open Access Journals (Sweden)

    I Ferrari

    1997-11-01

    Full Text Available Strategies to construct the physical map of the Trypanosoma cruzi nuclear genome have to capitalize on three main advantages of the parasite genome, namely (a its small size, (b the fact that all chromosomes can be defined, and many of them can be isolated by pulse field gel electrophoresis, and (c the fact that simple Southern blots of electrophoretic karyotypes can be used to map sequence tagged sites and expressed sequence tags to chromosomal bands. A major drawback to cope with is the complexity of T. cruzi genetics, that hinders the construction of a comprehensive genetic map. As a first step towards physical mapping, we report the construction and partial characterization of a T. cruzi CL-Brener genomic library in yeast artificial chromosomes (YACs that consists of 2,770 individual YACs with a mean insert size of 365 kb encompassing around 10 genomic equivalents. Two libraries in bacterial artificial chromosomes (BACs have been constructed, BACI and BACII. Both libraries represent about three genome equivalents. A third BAC library (BAC III is being constructed. YACs and BACs are invaluable tools for physical mapping. More generally, they have to be considered as a common resource for research in Chagas disease

  20. Versatile P(acman) BAC Libraries for Transgenesis Studies in Drosophila melanogaster

    Energy Technology Data Exchange (ETDEWEB)

    Venken, Koen J.T.; Carlson, Joseph W.; Schulze, Karen L.; Pan, Hongling; He, Yuchun; Spokony, Rebecca; Wan, Kenneth H.; Koriabine, Maxim; de Jong, Pieter J.; White, Kevin P.; Bellen, Hugo J.; Hoskins, Roger A.

    2009-04-21

    We constructed Drosophila melanogaster BAC libraries with 21-kb and 83-kb inserts in the P(acman) system. Clones representing 12-fold coverage and encompassing more than 95percent of annotated genes were mapped onto the reference genome. These clones can be integrated into predetermined attP sites in the genome using Phi C31 integrase to rescue mutations. They can be modified through recombineering, for example to incorporate protein tags and assess expression patterns.

  1. BAC Library Construction for Pumpkin Genome%南瓜基因组DNA的细菌人工染色体(BAC)文库的构建

    Institute of Scientific and Technical Information of China (English)

    赵茜; 张丽艳; 徐丽珍; 吴建忠

    2013-01-01

    [Objective] The paper was to construct the bacterial artificial chromosome (BAC) library for pumpkin genomic DNA.[Method] The BAC library of pumpkin genomic DNA was constructed by using pumpkin buds as materials and HindIII enzyme-cutting system.[Result] The BAC Library of pumpkin genomic DNA was constructed successfully.[Conclusion] The technology laid a foundation for the cloning and functional verification of related genes,physical mapping and genome sequencing of pumpkin.%[目的]构建南瓜基因组DNA的细菌人工染色体(BAC)文库.[方法]以南瓜幼芽为材料,利用HindⅢ酶切体系,初步构建南瓜基因组DNA的BAC文库.[结果]研究成功构建了南瓜基因组DNA的BAC文库.[结论]该技术为南瓜相关基因的克隆、功能验证、物理图谱的构建和基因组测序等研究工作奠定了基础.

  2. A Bac Library and Paired-PCR Approach to Mapping and Completing the Genome Sequence of Sulfolobus Solfataricus P2

    DEFF Research Database (Denmark)

    She, Qunxin; Confalonieri, F.; Zivanovic, Y.;

    2000-01-01

    -productive because there was a high sequence bias in the cosmid and lambda libraries. Therefore, a new approach was devised for linking the sequenced regions which may be generally applicable. BAC libraries were constructed and terminal sequences of the clones were determined and used for both end mapping and PCR...... screening. The PCR approaches included a novel chromosome walking method termed “paired-PCR”. 21 gaps were filled by BAC end sequence analyses and 6 gaps were filled by PCR including three large ones by paired-PCR. The complete map revealed that 0.9 Mb remained to be sequenced and 34 BAC clones were...... selected for walking over small gaps and preparing template libraries for larger ones. It is concluded that an optimal strategy for sequencing microorganism genomes involves construction of a high-resolution physical map by BAC end analyses, PCR screening and paired-PCR chromosome walking after about half...

  3. piggyBac mediates efficient in vivo CRISPR library screening for tumorigenesis in mice.

    Science.gov (United States)

    Xu, Chunlong; Qi, Xiaolan; Du, Xuguang; Zou, Huiying; Gao, Fei; Feng, Tao; Lu, Hengxing; Li, Shenglan; An, Xiaomeng; Zhang, Lijun; Wu, Yuanyuan; Liu, Ying; Li, Ning; Capecchi, Mario R; Wu, Sen

    2017-01-24

    CRISPR/Cas9 is becoming an increasingly important tool to functionally annotate genomes. However, because genome-wide CRISPR libraries are mostly constructed in lentiviral vectors, in vivo applications are severely limited as a result of difficulties in delivery. Here, we examined the piggyBac (PB) transposon as an alternative vehicle to deliver a guide RNA (gRNA) library for in vivo screening. Although tumor induction has previously been achieved in mice by targeting cancer genes with the CRISPR/Cas9 system, in vivo genome-scale screening has not been reported. With our PB-CRISPR libraries, we conducted an in vivo genome-wide screen in mice and identified genes mediating liver tumorigenesis, including known and unknown tumor suppressor genes (TSGs). Our results demonstrate that PB can be a simple and nonviral choice for efficient in vivo delivery of CRISPR libraries.

  4. Study on the Mitochondrial Genome of Sea Island Cotton (Gossypium barbadense) by BAC Library Screening

    Institute of Scientific and Technical Information of China (English)

    SU Ai-guo; LI Shuang-shuang; LIU Guo-zheng; LEI Bin-bin; KANG Ding-ming; LI Zhao-hu; MA Zhi-ying; HUA Jin-ping

    2014-01-01

    The plant mitochondrial genome displays complex features, particularly in terms of cytoplasmic male sterility (CMS). Therefore, research on the cotton mitochondrial genome may provide important information for analyzing genome evolution and exploring the molecular mechanism of CMS. In this paper, we present a preliminary study on the mitochondrial genome of sea island cotton (Gossypium barbadense) based on positive clones from the bacterial artiifcial chromosome (BAC) library. Thirty-ifve primers designed with the conserved sequences of functional genes and exons of mitochondria were used to screen positive clones in the genome library of the sea island cotton variety called Pima 90-53. Ten BAC clones were obtained and veriifed for further study. A contig was obtained based on six overlapping clones and subsequently laid out primarily on the mitochondrial genome. One BAC clone, clone 6 harbored with the inserter of approximate 115 kb mtDNA sequence, in which more than 10 primers fragments could be ampliifed, was sequenced and assembled using the Solexa strategy. Fifteen mitochondrial functional genes were revealed in clone 6 by gene annotation. The characteristics of the syntenic gene/exon of the sequences and RNA editing were preliminarily predicted.

  5. Physical analysis of the complex rye (Secale cereale L.) Alt4 aluminium (aluminum) tolerance locus using a whole-genome BAC library of rye cv. Blanco.

    Science.gov (United States)

    Shi, B-J; Gustafson, J P; Button, J; Miyazaki, J; Pallotta, M; Gustafson, N; Zhou, H; Langridge, P; Collins, N C

    2009-08-01

    Rye is a diploid crop species with many outstanding qualities, and is important as a source of new traits for wheat and triticale improvement. Rye is highly tolerant of aluminum (Al) toxicity, and possesses a complex structure at the Alt4 Al tolerance locus not found at the corresponding locus in wheat. Here we describe a BAC library of rye cv. Blanco, representing a valuable resource for rye molecular genetic studies, and assess the library's suitability for investigating Al tolerance genes. The library provides 6 x genome coverage of the 8.1 Gb rye genome, has an average insert size of 131 kb, and contains only ~2% of empty or organelle-derived clones. Genetic analysis attributed the Al tolerance of Blanco to the Alt4 locus on the short arm of chromosome 7R, and revealed the presence of multiple allelic variants (haplotypes) of the Alt4 locus in the BAC library. BAC clones containing ALMT1 gene clusters from several Alt4 haplotypes were identified, and will provide useful starting points for exploring the basis for the structural variability and functional specialization of ALMT1 genes at this locus.

  6. Construction of an Americn mink Bacterial Artificial Chromosome (BAC) library and sequencing candidate genes important for the fur industry

    DEFF Research Database (Denmark)

    Anistoroaei, Razvan Marian; Hallers, Boudewijn ten; Nefedov, Michael

    2011-01-01

    consisting of 18,432 clones spotted in duplicate, have been produced for hybridization screening and are publicly available. Overgo probes derived from expressed sequence tags (ESTs), representing 21 candidate genes for traits important for the mink industry, were used to screen the BAC library......BACKGROUND: Bacterial artificial chromosome (BAC) libraries continue to be invaluable tools for the genomic analysis of complex organisms. Complemented by the newly and fast growing deep sequencing technologies, they provide an excellent source of information in genomics projects. RESULTS: Here, we...... report the construction and characterization of the CHORI-231 BAC library constructed from a Danish-farmed, male American mink (Neovison vison). The library contains approximately 165,888 clones with an average insert size of 170 kb, representing approximately 10-fold coverage. High-density filters, each...

  7. A new implementation of high-throughput five-dimensional clone pooling strategy for BAC library screening

    Directory of Open Access Journals (Sweden)

    Deal Karin R

    2010-12-01

    Full Text Available Abstract Background A five-dimensional (5-D clone pooling strategy for screening of bacterial artificial chromosome (BAC clones with molecular markers utilizing highly-parallel Illumina GoldenGate assays and PCR facilitates high-throughput BAC clone and BAC contig anchoring on a genetic map. However, this strategy occasionally needs manual PCR to deconvolute pools and identify truly positive clones. Results A new implementation is reported here for our previously reported clone pooling strategy. Row and column pools of BAC clones are divided into sub-pools with 1~2× genome coverage. All BAC pools are screened with Illumina's GoldenGate assay and the BAC pools are deconvoluted to identify individual positive clones. Putative positive BAC clones are then further analyzed to find positive clones on the basis of them being neighbours in a contig. An exhaustive search or brute force algorithm was designed for this deconvolution and integrated into a newly developed software tool, FPCBrowser, for analyzing clone pooling data. This algorithm was used with empirical data for 55 Illumina GoldenGate SNP assays detecting SNP markers mapped on Aegilops tauschii chromosome 2D and Ae. tauschii contig maps. Clones in single contigs were successfully assigned to 48 (87% specific SNP markers on the map with 91% precision. Conclusion A new implementation of 5-D BAC clone pooling strategy employing both GoldenGate assay screening and assembled BAC contigs is shown here to be a high-throughput, low cost, rapid, and feasible approach to screening BAC libraries and anchoring BAC clones and contigs on genetic maps. The software FPCBrowser with the integrated clone deconvolution algorithm has been developed and is downloadable at http://avena.pw.usda.gov/wheatD/fpcbrowser.shtml.

  8. Association and in silico assignment of sequences from turkey BACs.

    Science.gov (United States)

    Reed, Kent M; Faile, Gretchen M; Kreuth, Stacy B; Chaves, Lee D; Sullivan, Laura M

    2008-01-01

    Bacterial artificial chromosomes (BACs) provide an important resource in genetic mapping. An initial set of BACs corresponding to microsatellite markers in the turkey (Meleagris gallopavo) was isolated from the CHORI-260 turkey BAC library. The selected markers were distributed on both macro- and microchromosomes and included a genetically unlinked marker. End sequences were obtained for a subset of the recovered BACs and compared to the chicken whole genome sequence. Close association of the turkey BAC-end sequences and original marker sequences was generally conserved in the chicken genome. Gene content of the turkey BACs is predicted from the comparative sequence alignments.

  9. Construction of an American mink Bacterial Artificial Chromosome (BAC library and sequencing candidate genes important for the fur industry

    Directory of Open Access Journals (Sweden)

    Christensen Knud

    2011-07-01

    Full Text Available Abstract Background Bacterial artificial chromosome (BAC libraries continue to be invaluable tools for the genomic analysis of complex organisms. Complemented by the newly and fast growing deep sequencing technologies, they provide an excellent source of information in genomics projects. Results Here, we report the construction and characterization of the CHORI-231 BAC library constructed from a Danish-farmed, male American mink (Neovison vison. The library contains approximately 165,888 clones with an average insert size of 170 kb, representing approximately 10-fold coverage. High-density filters, each consisting of 18,432 clones spotted in duplicate, have been produced for hybridization screening and are publicly available. Overgo probes derived from expressed sequence tags (ESTs, representing 21 candidate genes for traits important for the mink industry, were used to screen the BAC library. These included candidate genes for coat coloring, hair growth and length, coarseness, and some receptors potentially involved in viral diseases in mink. The extensive screening yielded positive results for 19 of these genes. Thirty-five clones corresponding to 19 genes were sequenced using 454 Roche, and large contigs (184 kb in average were assembled. Knowing the complete sequences of these candidate genes will enable confirmation of the association with a phenotype and the finding of causative mutations for the targeted phenotypes. Additionally, 1577 BAC clones were end sequenced; 2505 BAC end sequences (80% of BACs were obtained. An excess of 2 Mb has been analyzed, thus giving a snapshot of the mink genome. Conclusions The availability of the CHORI-321 American mink BAC library will aid in identification of genes and genomic regions of interest. We have demonstrated how the library can be used to identify specific genes of interest, develop genetic markers, and for BAC end sequencing and deep sequencing of selected clones. To our knowledge, this is the

  10. Giant panda BAC library construction and assembly of a 650-kb contig spanning major histocompatibility complex class II region

    Directory of Open Access Journals (Sweden)

    Pan Hui-Juan

    2007-09-01

    Full Text Available Abstract Background Giant panda is rare and endangered species endemic to China. The low rates of reproductive success and infectious disease resistance have severely hampered the development of captive and wild populations of the giant panda. The major histocompatibility complex (MHC plays important roles in immune response and reproductive system such as mate choice and mother-fetus bio-compatibility. It is thus essential to understand genetic details of the giant panda MHC. Construction of a bacterial artificial chromosome (BAC library will provide a new tool for panda genome physical mapping and thus facilitate understanding of panda MHC genes. Results A giant panda BAC library consisting of 205,800 clones has been constructed. The average insert size was calculated to be 97 kb based on the examination of 174 randomly selected clones, indicating that the giant panda library contained 6.8-fold genome equivalents. Screening of the library with 16 giant panda PCR primer pairs revealed 6.4 positive clones per locus, in good agreement with an expected 6.8-fold genomic coverage of the library. Based on this BAC library, we constructed a contig map of the giant panda MHC class II region from BTNL2 to DAXX spanning about 650 kb by a three-step method: (1 PCR-based screening of the BAC library with primers from homologous MHC class II gene loci, end sequences and BAC clone shotgun sequences, (2 DNA sequencing validation of positive clones, and (3 restriction digest fingerprinting verification of inter-clone overlapping. Conclusion The identifications of genes and genomic regions of interest are greatly favored by the availability of this giant panda BAC library. The giant panda BAC library thus provides a useful platform for physical mapping, genome sequencing or complex analysis of targeted genomic regions. The 650 kb sequence-ready BAC contig map of the giant panda MHC class II region from BTNL2 to DAXX, verified by the three-step method, offers a

  11. BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut

    Directory of Open Access Journals (Sweden)

    Chaine Christian

    2008-01-01

    Full Text Available Abstract Background Cultivated peanut, Arachis hypogaea is an allotetraploid of recent origin, with an AABB genome. In common with many other polyploids, it seems that a severe genetic bottle-neck was imposed at the species origin, via hybridisation of two wild species and spontaneous chromosome duplication. Therefore, the study of the genome of peanut is hampered both by the crop's low genetic diversity and its polyploidy. In contrast to cultivated peanut, most wild Arachis species are diploid with high genetic diversity. The study of diploid Arachis genomes is therefore attractive, both to simplify the construction of genetic and physical maps, and for the isolation and characterization of wild alleles. The most probable wild ancestors of cultivated peanut are A. duranensis and A. ipaënsis with genome types AA and BB respectively. Results We constructed and characterized two large-insert libraries in Bacterial Artificial Chromosome (BAC vector, one for each of the diploid ancestral species. The libraries (AA and BB are respectively c. 7.4 and c. 5.3 genome equivalents with low organelle contamination and average insert sizes of 110 and 100 kb. Both libraries were used for the isolation of clones containing genetically mapped legume anchor markers (single copy genes, and resistance gene analogues. Conclusion These diploid BAC libraries are important tools for the isolation of wild alleles conferring resistances to biotic stresses, comparisons of orthologous regions of the AA and BB genomes with each other and with other legume species, and will facilitate the construction of a physical map.

  12. Large-insert BAC/YAC libraries for selective re-isolation of genomic regions by homologous recombination in yeast.

    Science.gov (United States)

    Zeng, C; Kouprina, N; Zhu, B; Cairo, A; Hoek, M; Cross, G; Osoegawa, K; Larionov, V; de Jong, P

    2001-09-01

    We constructed representative large-insert bacterial artificial chromosome (BAC) libraries of two human pathogens (Trypanosoma brucei and Giardia lamblia) using a new hybrid vector, pTARBAC1, containing a yeast artificial chromosome (YAC) cassette (a yeast selectable marker and a centromere). The cassette allows transferring of BACs into yeast for their further modification. Furthermore, the new hybrid vector provides the opportunity to re-isolate each DNA insert without construction of a new library of random clones. Digestion of a BAC DNA by an endonuclease that has no recognition site in the vector, but which deletes most of the internal insert sequence and leaves the unique flanking sequences, converts a BAC into a TAR vector, thus allowing direct gene isolation. Cotransformation of a TAR vector and genomic DNA into yeast spheroplasts, and subsequent recombination between the TAR vector's flanking ends and a specific genomic fragment, allows rescue of the fragment as a circular YAC/BAC molecule. Here we prove a new cloning strategy by re-isolation of randomly chosen genomic fragments of different size from T. brucei cloned in BACs. We conclude that genomic regions of unicellular eukaryotes can be easily re-isolated using this technique, which provides an opportunity to study evolution of these genomes and the role of genome instability in pathogenicity.

  13. Construction of a nurse shark (Ginglymostoma cirratum bacterial artificial chromosome (BAC library and a preliminary genome survey

    Directory of Open Access Journals (Sweden)

    Inoko Hidetoshi

    2006-05-01

    Full Text Available Abstract Background Sharks are members of the taxonomic class Chondrichthyes, the oldest living jawed vertebrates. Genomic studies of this group, in comparison to representative species in other vertebrate taxa, will allow us to theorize about the fundamental genetic, developmental, and functional characteristics in the common ancestor of all jawed vertebrates. Aims In order to obtain mapping and sequencing data for comparative genomics, we constructed a bacterial artificial chromosome (BAC library for the nurse shark, Ginglymostoma cirratum. Results The BAC library consists of 313,344 clones with an average insert size of 144 kb, covering ~4.5 × 1010 bp and thus providing an 11-fold coverage of the haploid genome. BAC end sequence analyses revealed, in addition to LINEs and SINEs commonly found in other animal and plant genomes, two new groups of nurse shark-specific repetitive elements, NSRE1 and NSRE2 that seem to be major components of the nurse shark genome. Screening the library with single-copy or multi-copy gene probes showed 6–28 primary positive clones per probe of which 50–90% were true positives, demonstrating that the BAC library is representative of the different regions of the nurse shark genome. Furthermore, some BAC clones contained multiple genes, making physical mapping feasible. Conclusion We have constructed a deep-coverage, high-quality, large insert, and publicly available BAC library for a cartilaginous fish. It will be very useful to the scientific community interested in shark genomic structure, comparative genomics, and functional studies. We found two new groups of repetitive elements specific to the nurse shark genome, which may contribute to the architecture and evolution of the nurse shark genome.

  14. Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library

    Directory of Open Access Journals (Sweden)

    Seong-Lan Yu

    2012-01-01

    Full Text Available Pigs have been considered as donors for xenotransplantation in the replacement of human organs and tissues. However, porcine endogenous retroviruses (PERVs might transmit new infectious disease to humans during xenotransplantation. To investigate PERV integration sites, 45 PERV-positive BAC clones, including 12 PERV-A, 16 PERV-B, and 17 PERV-C clones, were identified from the NIH miniature pig BAC library. The analysis of 12 selected full-length sequences of PERVs, including the long terminal repeat (LTR region, identified the expected of open reading frame length, an indicative of active PERV, in all five PERV-C clones and one of the four PERV-B clones. Premature stop codons were observed in only three PERV-A clones. Also, eleven PERV integration sites were mapped using a 5000-rad IMpRH panel. The map locations of PERV-C clones have not been reported before, thus they are novel PERV clones identified in this study. The results could provide basic information for the elimination of site-specific PERVs in selection of pigs for xenotransplantation.

  15. Cytogenetical anchoring of sheep linkage map and syntenic groups using a sheep BAC library

    Directory of Open Access Journals (Sweden)

    Cribiu Edmond-Paul

    2000-07-01

    Full Text Available Abstract In order to simultaneously integrate linkage and syntenic groups to the ovine chromosomal map, a sheep bacterial artificial chromosome (BAC library was screened with previously assigned microsatellites using a sheep-hamster hybrid panel and genetic linkage. Thirty-three BACs were obtained, fluorescently labelled and hybridised on sheep-goat hybrid metaphases (2n = 57. This study allowed us, (i, to anchor all linkage groups on sheep chromosomes, (ii, to give information on the probable position of the centromere on the linkage map for the centromeric chromosomes, (iii, to contradict the previous orientation of the ovine × linkage group by the mapping of BMS1008 on OARXq38. Concerning our somatic cell hybrid panel, this study resulted in the assignment of all the previously unassigned groups to ovine chromosomes and a complete characterisation of the hybrid panel. In addition, since hybridisations were performed on a sheep-goat hybrid, new marker/anchoring points were added to the caprine cytogenetic map.

  16. Construction of an Americn mink Bacterial Artificial Chromosome (BAC) library and sequencing candidate genes important for the fur industry

    DEFF Research Database (Denmark)

    Anistoroaei, Razvan Marian; Hallers, Boudewijn ten; Nefedov, Michael;

    2011-01-01

    consisting of 18,432 clones spotted in duplicate, have been produced for hybridization screening and are publicly available. Overgo probes derived from expressed sequence tags (ESTs), representing 21 candidate genes for traits important for the mink industry, were used to screen the BAC library...

  17. Cross-species bacterial artificial chromosome (BAC) library screening via overgo-based hybridization and BAC-contig mapping of a yield enhancement quantitative trait locus (QTL) yld1.1 in the Malaysian wild rice Oryza rufipogon.

    Science.gov (United States)

    Song, Beng-Kah; Nadarajah, Kalaivani; Romanov, Michael N; Ratnam, Wickneswari

    2005-01-01

    The construction of BAC-contig physical maps is an important step towards a partial or ultimate genome sequence analysis. Here, we describe our initial efforts to apply an overgo approach to screen a BAC library of the Malaysian wild rice species, Oryza rufipogon. Overgo design is based on repetitive element masking and sequence uniqueness, and uses short probes (approximately 40 bp), making this method highly efficient and specific. Pairs of 24-bp oligos that contain an 8-bp overlap were developed from the publicly available genomic sequences of the cultivated rice, O. sativa, to generate 20 overgo probes for a 1-Mb region that encompasses a yield enhancement QTL yld1.1 in O. rufipogon. The advantages of a high similarity in melting temperature, hybridization kinetics and specific activities of overgos further enabled a pooling strategy for library screening by filter hybridization. Two pools of ten overgos each were hybridized to high-density filters representing the O. rufipogon genomic BAC library. These screening tests succeeded in providing 69 PCR-verified positive hits from a total of 23,040 BAC clones of the entire O. rufipogon library. A minimal tilling path of clones was generated to contribute to a fully covered BAC-contig map of the targeted 1-Mb region. The developed protocol for overgo design based on O. sativa sequences as a comparative genomic framework, and the pooled overgo hybridization screening technique are suitable means for high-resolution physical mapping and the identification of BAC candidates for sequencing.

  18. Cross-species bacterial artificial chromosome (BAC) library screening via overgo-based hybridization and BAC-contig mapping of a yield enhancement quantitative trait locus (QTL) yld1.1 in the Malaysian wild rice Oryza rufipogon

    OpenAIRE

    Song, Beng-Kah; Nadarajah, Kalaivani; Romanov, Michael N.; Ratnam, Wickneswari

    2005-01-01

    The construction of BAC-contig physical maps is an important step towards a partial or ultimate genome sequence analysis. Here, we describe our initial efforts to apply an overgo approach to screen a BAC library of the Malaysian wild rice species, Oryza rufipogon. Overgo design is based on repetitive element masking and sequence uniqueness, and uses short probes (approximately 40 bp), making this method highly efficient and specific. Pairs of 24-bp oligos that contain an 8-bp overlap were dev...

  19. Construction of a genome-wide human BAC-Unigene resource. Final progress report, 1989--1996

    Energy Technology Data Exchange (ETDEWEB)

    Lim, C.S.; Xu, R.X.; Wang, M. [and others

    1996-12-31

    Currently, over 30,000 mapped STSs and 27,000 mapped Unigenes (non-redundant, unigene sets of cDNA representing EST clusters) are available for human alone. A total of 44,000 Unigene cDNA clones have been supplied by Research Genetics. Unigenes, or cDNAs are excellent resource for map building for two reasons. Firstly, they exist in two alternative forms -- as both sequence information for PCR primer pairs, and cDNA clones -- thus making library screening by colony hybridization as well as pooled library PCR possible. The authors have developed an efficient and robust procedure to screen genomic libraries with large number of DNA probes. Secondly, the linkage and order of expressed sequences, or genes are highly conserved among human, mouse and other mammalian species. Therefore, mapping with cDNA markers rather than random anonymous STSs will greatly facilitate comparative, evolutionary studies as well as physical map building. They have currently deconvoluted over 10,000 Unigene probes against a 4X coverage human BAC clones from the approved library D by high density colony hybridization method. 10,000 batches of Unigenes are arrayed in an imaginary 100 X 100 matrix from which 100 row pools and 100 column pools are obtained. Library filters are hybridized with pooled probes, thus reducing the number of hybridization required for addressing the positives for each Unigene from 10,000 to 200. Details on the experimental scheme as well as daily progress report is posted on the Web site (http://www.tree.caltech.edu).

  20. Isolation of a 97-kb minimal essential MHC B locus from a new reverse-4D BAC library of the golden pheasant.

    Directory of Open Access Journals (Sweden)

    Qing Ye

    Full Text Available The bacterial artificial chromosome (BAC system is widely used in isolation of large genomic fragments of interest. Construction of a routine BAC library requires several months for picking clones and arraying BACs into superpools in order to employ 4D-PCR to screen positive BACs, which might be time-consuming and laborious. The major histocompatibility complex (MHC is a cluster of genes involved in the vertebrate immune system, and the classical avian MHC-B locus is a minimal essential one, occupying a 100-kb genomic region. In this study, we constructed a more effective reverse-4D BAC library for the golden pheasant, which first creates sub-libraries and then only picks clones of positive sub-libraries, and identified several MHC clones within thirty days. The full sequencing of a 97-kb reverse-4D BAC demonstrated that the golden pheasant MHC-B locus contained 20 genes and showed good synteny with that of the chicken. The notable differences between these two species were the numbers of class II B loci and NK genes and the inversions of the TAPBP gene and the TAP1-TAP2 region. Furthermore, the inverse TAP2-TAP1 was unique in the golden pheasant in comparison with that of chicken, turkey, and quail. The newly defined genomic structure of the golden pheasant MHC will give an insight into the evolutionary history of the avian MHC.

  1. Repetitive Genomic Elements in a European Corn Borer, Ostrinia nubilalis, BAC Library were Indicated by BAC End Sequencing and Development of Sequence Tag Site Markers: Implications for Lepidopteran Genomic Research

    Science.gov (United States)

    The European corn borer, Ostrinia nubilalis, is a serious pest of food, fiber, and biofuel crops in Europe, North America, and Asia, and a model system for insect olfaction and speciation. A bacterial artificial chromosome (BAC) library constructed for O. nubilalis contains 36,864 clones with estim...

  2. Development of four phylogenetically-arrayed BAC libraries and sequence of the APA locus in Phaseolus vulgaris.

    Science.gov (United States)

    Kami, James; Poncet, Valérie; Geffroy, Valérie; Gepts, Paul

    2006-04-01

    The APA family of seed proteins consists of three subfamilies, in evolutionary order of hypothesized appearance: phytohaemagglutinins (PHA), alpha-amylase inhibitors (alphaAI), and arcelins (ARL). The APA family plays a defensive role against mammalian and insect seed predation in common bean (Phaseolus vulgaris L.). The main locus (APA) for this gene family is situated on linkage group B4. In order to elucidate the pattern of duplication and diversification at this locus, we developed a BAC library in each of four different Phaseolus genotypes that represent presumptive steps in the evolutionary diversification of the APA family. Specifically, BAC libraries were established in one P. lunatus (cv. 'Henderson: PHA+ alphaAI- ARL-) and three P. vulgaris accessions (presumed ancestral wild G21245 from northern Peru: PHA+ alphaAI+ ARL-; Mesoamerican wild G02771: PHA+ alphaAI+ ARL+; and Mesoamerican breeding line BAT93: PHA+ alphaAI+ ARL-). The libraries were constructed after HindIII digestion of high molecular weight DNA, obtained with a novel nuclei isolation procedure. The frequency of empty or cpDNA-sequence-containing clones in all libraries is low (generally APA gene family, including members of the three subfamilies, as hypothesized. The different subfamilies were interspersed with retrotransposon sequences. In addition, other sequences were identified with similarity to chloroplast DNA, a dehydrin gene, and the Arabidopsis flowering D locus. Linkage between the dehydrin gene and the D1711 RFLP marker identifies a potential syntenic region between parts of common bean linkage group B4 and cowpea linkage group 2.

  3. Library Resource Categories and Their Possible Groupings

    Science.gov (United States)

    Hider, Philip

    2009-01-01

    The resource typologies proposed in the new standard, "Resource Description and Access" (RDA), are evaluated in the context of a particular university library catalogue through two card-sort exercises. Although it was found that end-users recognised the content and carrier aspects of the resource types as listed in RDA, they did not categorise…

  4. Reviewing the Electronic Resources & Libraries Conference

    Science.gov (United States)

    Tijerina, Bonnie

    2008-01-01

    The third Electronic Resources & Libraries (ER&L) conference gathered at Georgia Institute of Technology's Global Learning and Conference Center in Atlanta, Georgia, March 18-21, 2008. Over 360 attendees, from six countries and from 80% of the United States, represented their libraries and organizations resulting in a diverse and…

  5. Managing Academic Libraries with Fewer Resources.

    Science.gov (United States)

    Riggs, Donald E.

    1992-01-01

    A discussion of academic library management during retrenchment looks at a variety of issues, including staffing needs in the labor-intensive library environment, acquisitions budgeting, interlibrary cooperation (ownership vs. access to resources), entrepreneurship and strategic planning for problem solving, and use of total quality management…

  6. Construction of BAC Library for Egyptian Cotton Varieties%埃及棉花品种的BAC文库构建

    Institute of Scientific and Technical Information of China (English)

    Osama MOMTAZ; Abeer IBRAHIM

    2002-01-01

    @@ BAC library for the Egyptian cotton Gossypium barbadense Giza 70, Giza 86, and Giza 75varieties have been constructed and characterized. The isolation and purification of high molecular weight DNA from nuclei embedded in agarose microbeads was an essential part of this work.

  7. PCR-based Screening BAC Library and Direct End Sequencing of BAC Clones%PCR筛选BAC文库和直接BAC末端测序方法的建立

    Institute of Scientific and Technical Information of China (English)

    何聪芬; 小松田隆夫

    2004-01-01

    A PCR based strategy was developed which required four steps to identify positive BAC clones from barley BAC(bacterial artificial chromosome)library.In the protocol,two levels of BAC DNA pools(super-pool and pool)were prepared for analysis.One pool is made of one plate DNAs and one super-pool is made of mixing ten consecutive 1/100 diluted pool DNAs(1~10,11~20 ect).First,super-pool DNAs were analysed and then 10 pool DNAs contained in every positive super-pool were analysed.Once positive BAC plates were identified,the bacterial cultures were dipped into PCR mixtures and reaction is made to identify positive BAC clones.The BAC clones identified by each marker were grouped into contigs.BAC-end sequence was obtained from BACs within each contig and primers were designed for the next step chromosome walking.In case of the BAC ends belong to repetitive sequence,the primers were designed based on the subcloned unique band in the contig(identified by Hind III digestion pattern).This method allows us to construct the BAC contig without the costly and time-consuming efforts,and no radioactivity harmful to the body.%建立了一种用PCR方法筛选富含高度重复序列的大麦BAC DNA 文库和直接对 BAC DNA进行末端测序的方法.用PCR技术进行大麦BAC DNA 文库(含816个平板,每个平板含384个克隆)的筛选分4步进行.在实验中,建立了两个水平的BAC DNA池(一级池和二级池).一个二级池由一个平板(含有384个克隆)的DNA 组成,一个一级池由连续10个稀释100倍的二级池的DNA混合而成(如1~10,11~20等),共82个一级池.BAC DNA 文库筛选的第一步是对82个一级池的筛选.得到阳性一级池后(如2号一级池),对其所含的10个二级池(从11~20)进行第二步筛选.得到阳性二级池后,培养相应的阳性平板的所有克隆(384个),从头开始(左上侧),每相邻的4个克隆为一组,在96孔板上(4 X 96=384) 进行第三轮PCR反应;之后对筛选结果为阳性的4

  8. Integration of chicken genomic resources to enable whole-genome sequencing

    NARCIS (Netherlands)

    Aerts, J.A.; Crooijmans, R.P.M.A.; Cornelissen, S.J.B.; Hemmatian, K.; Veenendaal, A.; Jaader, A.; Poel, van der J.J.; Fillon, V.; Vignal, I.; Groenen, M.A.M.

    2003-01-01

    Different genomic resources in chicken were integrated through the Wageningen chicken BAC library. First, a BAC anchor map was created by screening this library with two sets of markers: microsatellite markers from the consensus linkage map and markers created from BAC end sequencing in chromosome w

  9. Resource Discovery within the Networked "Hybrid" Library.

    Science.gov (United States)

    Leigh, Sally-Anne

    This paper focuses on the development, adoption, and integration of resource discovery, knowledge management, and/or knowledge sharing interfaces such as interactive portals, and the use of the library's World Wide Web presence to increase the availability and usability of information services. The introduction addresses changes in library…

  10. A five-fold pig bacterial artificial chromosome library:a resource for positional cloning and physical mapping

    Institute of Scientific and Technical Information of China (English)

    LIU Wei; LI Ning; ZHANG Ying; LIU Zhaoliang; GUO Li; WANG Xiaobo; FEI Jing; FENG Jidong; ZHAO Rui; HU Xiaoxiang

    2006-01-01

    A pig BAC library was constructed with genomic DNA from a male Erhualian pig. After partial digestion with Hind Ⅲ or BamH I the fragments obtained were cloned into the pBeloBAC11 vector. The library consists of 184320 clones which stored in 480pieces 384-well plates (20 plates per superpool). A two-step 4-dimension PCR screening system was established to screen the positive clones. An average insert size of 128 kb was estimated from 105 randomly isolated clones, which indicates that the library is more than five times of genomic coverage. For the demonstration of the probability to pick out any unique genes or DNA markers from the library, 10single-copy genes were screened out and the positive clones were yielded between 1 and 8 with an average of 3.6. Positive superpools were obtained for 32 microsatellite markers selected from different regions of pig genome. The number of positive superpools for each marker varies from 1 to 9 with an average of 4.78. This BAC library provides an additional resource for pig physical mapping and gene identification.

  11. Integrating Resources in the Education Library: Trends, Issues, and Reality

    Science.gov (United States)

    Osa, Justina O.

    2005-01-01

    Resources found in the typical education library that supports teacher education programs often include print and non-print library items, and other items that are unique to education library collections. This article attempts to share what the education library is doing to integrate all of its resources irrespective of their formats. The main…

  12. Construction and characterization of large-insert genomic libraries (BAC and fosmid) from the Ascidian Botryllus schlosseri and initial physical mapping of a histocompatibility locus.

    Science.gov (United States)

    de Tomaso, Anthony W; Weissman, Irving L

    2003-01-01

    The colonial protochordate Botryllus schlosseri is genetically manipulable and represents a potential model organism for a variety of biological disciplines, including immunology, stem cell biology and development. This article presents the construction and characterization of both BAC and fosmid genomic libraries of the 725-Mbp B. schlosseri genome. The BAC library currently consists of 2x genome coverage with an average insert size of 80 kb. The fosmid library is at 11x genome coverage with an average insert of 40 kb. B. schlosseri is a small organism containing a large number of compounds that hinder DNA purification. Thus a number of protocols had to be modified in order to make purified, high molecular weight inserts for cloning, including both gel purification and insert concentration techniques. Both libraries were characterized by using them in initial physical mapping of a single histocompatibility locus, and were found to be representative and functional. These libraries are important tools for physical mapping and positional cloning in the B. schlosseri genome, and the techniques adapted to make them are suitable for use on other organisms in which high molecular weight DNA is difficult to purify.

  13. Bac clones generated from sheared dna

    Energy Technology Data Exchange (ETDEWEB)

    Osoegawa, Kazutoyo; Vessere, Gery M.; Shu, Chung Li; Hoskins,Roger A.; Abad, Jose P.; de Pablos, Beatriz; Villasante, Alfredo; deJong, Pieter J.

    2006-08-09

    BAC libraries generated from restriction-digested genomic DNA display representational bias and lack some sequences. To facilitate completion of genome projects, procedures have been developed to create BACs from DNA physically sheared to create fragments extending up to 200kb. The DNA fragments were repaired to create blunt ends and ligated to a new BAC vector. This approach has been tested by generating BAC libraries from Drosophila DNA, with insert lengths of 50 kb to 150 kb. The libraries lack chimeric clone problems as determined by mapping paired BAC-end sequences of one library to the D. melanogaster genome sequence. The utility of ''sheared'' libraries was demonstrated by closure of a previous clone gap and by isolation of clones from telomeric regions, which were notably absent from previous Drosophila BAC libraries.

  14. 中国水仙BAC文库构建的研究%Construction and Characteristics of a BAC Library of Narcissus tazetta var.chinensis

    Institute of Scientific and Technical Information of China (English)

    王江波; 潘东明; 潘腾飞; 郭志雄

    2012-01-01

    To further study Narcissus genome and screen functional genes, a BAC library was constructed for the first time in the world. The high molecular weight DNA (HMW-DNA) was isolated from Naivissus etiolation leaves used improved Zhang' s method. The HMW-DNA was cut by Hind III and ligated to BAC vectors; the ligations were transformed into Escherichia colt DH10B. The results showed that the DNA isolated by improved Zhang' s method was higher than 1 Mb, which was suitable for BAC library construction. The ligation and transform methods were improved; the optimum mole ratio between vectors and DNA fragments was 5:1. The library consists of 69120 clones with an average insert size of 87 kb, about 50% clones contain 80 to 90 kb insert DNA, and less than 1% of the clones do not contain inserts. Screening of BAC library with chloroplast DNA probes by Southern blot indicated that less than 1 % of the clones contained organelle-derived DNA.%为更深入地挖掘中国水仙的基因组信息,筛选与中国水仙品质相关的功能基因,以中国水仙品种‘金盏银台’的黄化叶片为材料,采用改良Zhang法获得高分子量核DNA,经酶切、连接和转化,首次构建了中国水仙基因组BAC文库.结果表明,采用改良zhang法获取的核DNA分子量大于1Mb,适合BAC文库的构建;优化了连接、转化体系,发现载体和插入片段的摩尔比为5∶1时,连接、转化效率最高;经检测,该文库包括69120个克隆,插入片段的平均大小约87 kb,空载率小于1%,大约50%的克隆大小在80~90kb之间;Southern blot结果表明该文库未受到细胞器DNA的污染.

  15. 应用随机多肽文库分析BAC5单抗相关的抗原表位%Analysis of BAC5 mcAb-Related Epitope Using Random Peptide library

    Institute of Scientific and Technical Information of China (English)

    肖锡宾; 张昌卿; 张颖; 张如华; 李经略; 冯凯涛; 孙韵; 叶永照

    2003-01-01

    Objective To identify epitope relating to BAC5 mcAb, a kind of monoclonal antibody (mcAb) located on the surface of nasopha-ryngeal carcinoma (NPC) cells.Methods Using BAC5 mcAb as a selected target, the 3 rounds of biopanning to a 12 mer random peptide library (RPL) presen-ted by M13 phages were carried out. The positive M13 phage clones were chosen and confirmed with sandwich ELISA for antibodycapture and competitive assay. The exogenous DNA fragments in the positive/negative M13 phages were sequenced to deduce andcompare the order of the amino acids of exogenous peptides among the phage clones.Results 77% (35/45) of the phages eluted from the 3rd round of biopnning could be captured by BAC5 mcAb. The 3 kinds ofthe peptides were displayed by M13 phages from the 8 positive clones identified with competitive assay. The same character of "-P-V-"structure existed near N-terminus of the 3 different peptides, i.e. -H-Q-S-H-Y-P-Y-P-V-V-S-L- (4/8) -Q-N-Q-A-W-F-S-Q-P-V-R-M- (3/8) and T-Q-A-Y-K-G-F-P-V-L-P-S- (1/8) in comparison with the peptide" -N-H-Q-S-T-F-W-Q-K-W-T-A-" dis-played by M13 phages from the negative clones (6/6).Conclusion BAC5 mcAb can recognize the 3 kinds of the peptides with-P-V-structure near N-terminus. These peptides mimic thestructure of the epitope on the surface of NPC cells recognized by BAC5 mcAb.%目的 鉴定能被BAC5单抗识别的定位于鼻咽癌细胞表面的抗原表位.方法 应用BAC5单抗作为靶抗体对噬菌体呈现的随机12肽文库进行3轮生物淘洗,用抗体捕获和竞争试验的夹心ELISA方法选择和鉴定阳性噬菌体克隆,对阳性和阴性噬菌体的外源性DNA片段进行序列分析,推导和比较由这些噬菌体所呈现的多肽氨基酸序列.结果 通过3轮生物淘洗能被抗体捕获的噬菌体克隆为77%(35/45).用竞争试验从所捕获的克隆中测得8个阳性克隆.来自这8个克隆的噬菌体呈现三种外源多肽,即-H-Q-S-H-Y-P-Y-P-V-V-S-L-(4/8)-Q-N-Q-A

  16. Identification of an extensive gene cluster among a family of PPOs in Trifolium pratense L. (red clover using a large insert BAC library

    Directory of Open Access Journals (Sweden)

    Thomas Ann

    2009-07-01

    Full Text Available Abstract Background Polyphenol oxidase (PPO activity in plants is a trait with potential economic, agricultural and environmental impact. In relation to the food industry, PPO-induced browning causes unacceptable discolouration in fruit and vegetables: from an agriculture perspective, PPO can protect plants against pathogens and environmental stress, improve ruminant growth by increasing nitrogen absorption and decreasing nitrogen loss to the environment through the animal's urine. The high PPO legume, red clover, has a significant economic and environmental role in sustaining low-input organic and conventional farms. Molecular markers for a range of important agricultural traits are being developed for red clover and improved knowledge of PPO genes and their structure will facilitate molecular breeding. Results A bacterial artificial chromosome (BAC library comprising 26,016 BAC clones with an average 135 Kb insert size, was constructed from Trifolium pratense L. (red clover, a diploid legume with a haploid genome size of 440–637 Mb. Library coverage of 6–8 genome equivalents ensured good representation of genes: the library was screened for polyphenol oxidase (PPO genes. Two single copy PPO genes, PPO4 and PPO5, were identified to add to a family of three, previously reported, paralogous genes (PPO1–PPO3. Multiple PPO1 copies were identified and characterised revealing a subfamily comprising three variants PPO1/2, PPO1/4 and PPO1/5. Six PPO genes clustered within the genome: four separate BAC clones could be assembled onto a predicted 190–510 Kb single BAC contig. Conclusion A PPO gene family in red clover resides as a cluster of at least 6 genes. Three of these genes have high homology, suggesting a more recent evolutionary event. This PPO cluster covers a longer region of the genome than clusters detected in rice or previously reported in tomato. Full-length coding sequences from PPO4, PPO5, PPO1/5 and PPO1/4 will facilitate

  17. Integrating print and digital resources in library collections

    CERN Document Server

    Katz, Linda S

    2013-01-01

    Get informed answers to your questions and concerns about integrating the materials in your library's collectionLibrary collections have always included materials in many formats?handling a mix of material types is an accepted part of library work. And in recent years, the very concept of ?collection? has been significantly redefined by the addition of electronic resources. But are print and digital materials really merged in library collections or are they treated and maintained as separate entities? Integrating Print and Digital Resources in Library Collections examines a variety

  18. Library Resources and Services for Faculty.

    Science.gov (United States)

    Bonn, Robert; And Others

    This pamphlet was designed to acquaint faculty at the John Jay College of Criminal Justice with general non-criminal justice services available in the college library for their own research and study. Emphasis is placed on using the reference collection of a small library and the expertise of the library staff to identify and locate information…

  19. Resource sharing in libraries concepts, products, technologies, and trends

    CERN Document Server

    Breeding, Marshall

    2014-01-01

    Supplementing your local collection through resource sharing is a smart way to ensure your library has the resources to satisfy the needs of your users. Marshall Breeding's new Library Technology Report explores technologies and strategies for sharing resources, helping you streamline workflows and improve resource-sharing services by covering key strategies like interlibrary loan, consortial borrowing, document delivery, and shared collections. You'll also learn about such trends and services as:OCLC WorldCat Resource Sharing, and other systems that facilitate cooperative, reciprocal lendingS

  20. Library Support for Resource Constrained Accelerators

    DEFF Research Database (Denmark)

    Brock-Nannestad, Laust; Karlsson, Sven

    2014-01-01

    . In this paper, we present a lightweight system where an accelerator can remotely execute library functions on a host processor. The implementation takes up 750 bytes but can replace arbitrary library calls leading to significant savings in memory foot print. We evaluate with a set of SPLASH-2 applications...

  1. Multi-registration of software library resources

    Science.gov (United States)

    Archer, Charles J.; Blocksome, Michael A.; Ratterman, Joseph D.; Smith, Brian E.

    2011-04-05

    Data communications, including issuing, by an application program to a high level data communications library, a request for initialization of a data communications service; issuing to a low level data communications library a request for registration of data communications functions; registering the data communications functions, including instantiating a factory object for each of the one or more data communications functions; issuing by the application program an instruction to execute a designated data communications function; issuing, to the low level data communications library, an instruction to execute the designated data communications function, including passing to the low level data communications library a call parameter that identifies a factory object; creating with the identified factory object the data communications object that implements the data communications function according to the protocol; and executing by the low level data communications library the designated data communications function.

  2. A study of weeding policies in eleven TALON resource libraries.

    Science.gov (United States)

    Goldstein, C H

    1981-07-01

    A study was made of the weeding policies and practices of eleven TALON resource libraries. The results indicated that although weeding, or collection evaluation as it is also known, was performed by most of the libraries, few had a written policy. The reasons for weeding and the types of weeding done by the libraries are described. A discussion of the prevalent means of disposition of withdrawn materials and of the obstacles to cooperative weeding is included.

  3. A study of weeding policies in eleven TALON resource libraries.

    OpenAIRE

    Goldstein, C H

    1981-01-01

    A study was made of the weeding policies and practices of eleven TALON resource libraries. The results indicated that although weeding, or collection evaluation as it is also known, was performed by most of the libraries, few had a written policy. The reasons for weeding and the types of weeding done by the libraries are described. A discussion of the prevalent means of disposition of withdrawn materials and of the obstacles to cooperative weeding is included.

  4. A study of weeding policies in eleven TALON resource libraries.

    Science.gov (United States)

    Goldstein, C H

    1981-01-01

    A study was made of the weeding policies and practices of eleven TALON resource libraries. The results indicated that although weeding, or collection evaluation as it is also known, was performed by most of the libraries, few had a written policy. The reasons for weeding and the types of weeding done by the libraries are described. A discussion of the prevalent means of disposition of withdrawn materials and of the obstacles to cooperative weeding is included. PMID:7248594

  5. The Library Search Engine: A Smart Solution for Integrating Resources Beyond Library Holdings

    Directory of Open Access Journals (Sweden)

    Karin Herm

    2008-09-01

    Full Text Available The Cooperative Library Network Berlin-Brandenburg (KOBV, Germany addresses the problem of how to integrate resources found outside the library and library holdings into a single discovery tool. It presents a solution that uses open source technology to develop a next-generation catalog interface called the Library Search Engine. This pilot project was launched in 2007 with the library of Albert Einstein Science Park, Potsdam. The idea was to design and develop a fast and convenient search tool, integrating local holdings (books, journals, journal articles as well as relevant scientific subject information such as open access publications and bibliographies.

  6. Library Programs: Interlibrary Cooperation and Resource Sharing. Fiscal Year 1987.

    Science.gov (United States)

    Kittel, Dorothy

    This overview of how the 50 states used their Library Services and Construction Act funds under Title III (Interlibrary Cooperation and Resource Sharing) in fiscal year 1987 reports that, overall, state library agencies spent $16 million in Title III funds to work toward the creation of statewide databases of the bibliographic holdings of all…

  7. Resource Provisions of a High School Library Collection

    Science.gov (United States)

    Collins, Karla B.; Doll, Carol A.

    2012-01-01

    The mission of the school library "is to ensure students and staff are effective users of ideas and information" (AASL 2009, 8). The school library collection should, therefore, support instruction throughout the school. However, teachers do not always understand the potential value of the resources available. This research explored…

  8. Resource Allocation to Government Documents Departments in Academic Libraries.

    Science.gov (United States)

    Cook, Kevin L.

    1985-01-01

    Explores both the allocation of resources to documents departments in academic libraries and the wide variation in local levels of support. Ways in which documents librarians may change levels of support through sources within and beyond their libraries are suggested. (CLB)

  9. National Library of Medicine Web Resources for Student Health Professionals

    Energy Technology Data Exchange (ETDEWEB)

    Womble, R.

    2010-04-02

    Familiarize students affiliated with the Student National Medical Association with the National Library of Medicine's online resources that address medical conditions, health disparities, and public health preparedness needs.

  10. Gauging User Interest in Non-Traditional Library Resources

    Energy Technology Data Exchange (ETDEWEB)

    Sandberg, Tami; Abbott, Jennifer

    2015-06-23

    The National Renewable Energy Laboratory (NREL) is a government funded research laboratory based in Golden, Colorado. In addition to collecting traditional library resources such as journals, conference proceedings, and print and electronic books, the library also spends a significant portion of its collection development funds on resources not often found in many libraries: technical industry standards (e.g., ISO, IEC, ASTM, IEEE) and energy-related market reports. Assessing user needs for these resources is difficult for a number of reasons, particularly because standardized usage statistics are lacking or non-existent. Standards and market reports are generally costly and include fairly restrictive license agreements, which increase the importance of making informed collection development decisions. This presentation will discuss the NREL Library's current collection assessment and development practices as they relate to these unique resources.

  11. A genome assembly-integrated dog 1 Mb BAC microarray: a cytogenetic resource for canine cancer studies and comparative genomic analysis.

    Science.gov (United States)

    Thomas, R; Duke, S E; Karlsson, E K; Evans, A; Ellis, P; Lindblad-Toh, K; Langford, C F; Breen, M

    2008-01-01

    Molecular cytogenetic studies have been instrumental in defining the nature of numerical and structural chromosome changes in human cancers, but their significance remains to be fully understood. The emergence of high quality genome assemblies for several model organisms provides exciting opportunities to develop novel genome-integrated molecular cytogenetic resources that now permit a comparative approach to evaluating the relevance of tumor-associated chromosome aberrations, both within and between species. We have used the dog genome sequence assembly to identify a framework panel of 2,097 bacterial artificial chromosome (BAC) clones, selected at intervals of approximately one megabase. Each clone has been evaluated by multicolor fluorescence in situ hybridization (FISH) to confirm its unique cytogenetic location in concordance with its reported position in the genome assembly, providing new information on the organization of the dog genome. This panel of BAC clones also represents a powerful cytogenetic resource with numerous potential applications. We have used the clone set to develop a genome-wide microarray for comparative genomic hybridization (aCGH) analysis, and demonstrate its application in detection of tumor-associated DNA copy number aberrations (CNAs) including single copy deletions and amplifications, regional aneuploidy and whole chromosome aneuploidy. We also show how individual clones selected from the BAC panel can be used as FISH probes in direct evaluation of tumor karyotypes, to verify and explore CNAs detected using aCGH analysis. This cytogenetically validated, genome integrated BAC clone panel has enormous potential for aiding gene discovery through a comparative approach to molecular oncology.

  12. Recombining overlapping BACs into single large BACs.

    Science.gov (United States)

    Kotzamanis, George; Kotsinas, Athanassios

    2015-01-01

    BAC clones containing the entire genomic region of a gene including the long-range regulatory elements are very useful for gene functional analysis. However, large genes often span more than the insert of a BAC clone, and single BACs covering the entire region of interest are not available. Here, we describe a general system for linking two or more overlapping BACs into a single clone. Two rounds of homologous recombination are used. In the first, the BAC inserts are subcloned into the pBACLink vectors. In the second, the two BACs are combined together. Multiple BACs in a contig can be combined by alternating use of the pBACLInk vectors, resulting in several BAC clones containing as much of the genomic region of a gene as required. Such BACs can then be used in gene expression studies and/or gene therapy applications.

  13. 棉花BAC文库快速筛选法%A Rapid Method of Screening BAC Library in Cotton

    Institute of Scientific and Technical Information of China (English)

    程华; 彭仁海; 张香娣; 刘方; 王春英; 王坤波

    2012-01-01

    目的:构建棉花细菌人工染色体(Bacterial Artificial Chromosome,BAC)文库的快速筛选法,以期从BAC文库中大量、快速、高效筛选出特定BAC克隆,为从事基因组测序、分离和分析特定基因、构建物理图谱及基因图位克隆等生物学技术研究奠定基础.方法:构建了整板、行、列的三维混合池,以菌液PCR为基础,从BAC文库中筛选出含有特定DNA片段的BAC单克隆.结果:从BAC文库的3 456个克隆中,共筛选出16个阳性单克隆,涉及13条染色体、11个SSR标记.结论:该文构建的棉花BAC文库筛选体系,筛选快速、准确,适合从BAC文库中大量筛选BAC单克隆.结合当前的多种BAC文库筛选方法进行探讨,根据不同的实验目的选择更合适的筛选方法和操作步骤.

  14. Resource discovery in distributed digital libraries through visual knowledge navigation

    Institute of Scientific and Technical Information of China (English)

    GU Qian-yi; AHMAD Faisal; SUMNER Tamara

    2005-01-01

    In order to support users to search and browse for various resources, digital libraries are composed of discovery systems which provide user interface and information retrieve system. Recent researches in Information Retrieval have investigated different techniques through improving precision and recall to enhance the effectiveness of discovery system in digital libraries. In this paper, we present our work to enhance discovery system effectiveness with a different approach, through resource discovery based on visual knowledge navigation. In our Strand Map Services project under National Science Digital Library, we introduce the visual resource discovery system called conceptual browsing interfaces, to help educators and learners to locate,comprehend and use educational resources in digital libraries. The paper begins with a short introduction of the Strand Map Services. Then we illustrate the service architecture, the design and implementation of its major components. We will focus our discussion of how the visualization system of the Strand Map Services supports the visual knowledge navigation for distributed digital libraries. This includes the knowledge acquisition of the conceptual browsing interfaces, different knowledge representations in the system perspective and user interface perspective, visualization system modules, algorithm and Web services integration to use visual knowledge navigation to enhance resource discovery in digital libraries.

  15. Integrated and sequence-ordered BAC- and YAC-based physical maps for the rat genome.

    Science.gov (United States)

    Krzywinski, Martin; Wallis, John; Gösele, Claudia; Bosdet, Ian; Chiu, Readman; Graves, Tina; Hummel, Oliver; Layman, Dan; Mathewson, Carrie; Wye, Natasja; Zhu, Baoli; Albracht, Derek; Asano, Jennifer; Barber, Sarah; Brown-John, Mabel; Chan, Susanna; Chand, Steve; Cloutier, Alison; Davito, Jonathon; Fjell, Chris; Gaige, Tony; Ganten, Detlev; Girn, Noreen; Guggenheimer, Kurtis; Himmelbauer, Heinz; Kreitler, Thomas; Leach, Stephen; Lee, Darlene; Lehrach, Hans; Mayo, Michael; Mead, Kelly; Olson, Teika; Pandoh, Pawan; Prabhu, Anna-Liisa; Shin, Heesun; Tänzer, Simone; Thompson, Jason; Tsai, Miranda; Walker, Jason; Yang, George; Sekhon, Mandeep; Hillier, LaDeana; Zimdahl, Heike; Marziali, Andre; Osoegawa, Kazutoyo; Zhao, Shaying; Siddiqui, Asim; de Jong, Pieter J; Warren, Wes; Mardis, Elaine; McPherson, John D; Wilson, Richard; Hübner, Norbert; Jones, Steven; Marra, Marco; Schein, Jacqueline

    2004-04-01

    As part of the effort to sequence the genome of Rattus norvegicus, we constructed a physical map comprised of fingerprinted bacterial artificial chromosome (BAC) clones from the CHORI-230 BAC library. These BAC clones provide approximately 13-fold redundant coverage of the genome and have been assembled into 376 fingerprint contigs. A yeast artificial chromosome (YAC) map was also constructed and aligned with the BAC map via fingerprinted BAC and P1 artificial chromosome clones (PACs) sharing interspersed repetitive sequence markers with the YAC-based physical map. We have annotated 95% of the fingerprint map clones in contigs with coordinates on the version 3.1 rat genome sequence assembly, using BAC-end sequences and in silico mapping methods. These coordinates have allowed anchoring 358 of the 376 fingerprint map contigs onto the sequence assembly. Of these, 324 contigs are anchored to rat genome sequences localized to chromosomes, and 34 contigs are anchored to unlocalized portions of the rat sequence assembly. The remaining 18 contigs, containing 54 clones, still require placement. The fingerprint map is a high-resolution integrative data resource that provides genome-ordered associations among BAC, YAC, and PAC clones and the assembled sequence of the rat genome.

  16. Graphic Resources in the Spanish Art Libraries.

    Science.gov (United States)

    Medina, Alicia Garcia; Coso, Teresa

    The interest in graphic information has increased explosively and is explored through various communication networks like the Internet and the audiovisual media. Art libraries, museums, cultural centers, and art foundations manage and store a lot of images in several departments and in print and digital formats. This paper discusses the cataloging…

  17. Open resource metagenomics: a model for sharing metagenomic libraries.

    Science.gov (United States)

    Neufeld, J D; Engel, K; Cheng, J; Moreno-Hagelsieb, G; Rose, D R; Charles, T C

    2011-11-30

    Both sequence-based and activity-based exploitation of environmental DNA have provided unprecedented access to the genomic content of cultivated and uncultivated microorganisms. Although researchers deposit microbial strains in culture collections and DNA sequences in databases, activity-based metagenomic studies typically only publish sequences from the hits retrieved from specific screens. Physical metagenomic libraries, conceptually similar to entire sequence datasets, are usually not straightforward to obtain by interested parties subsequent to publication. In order to facilitate unrestricted distribution of metagenomic libraries, we propose the adoption of open resource metagenomics, in line with the trend towards open access publishing, and similar to culture- and mutant-strain collections that have been the backbone of traditional microbiology and microbial genetics. The concept of open resource metagenomics includes preparation of physical DNA libraries, preferably in versatile vectors that facilitate screening in a diversity of host organisms, and pooling of clones so that single aliquots containing complete libraries can be easily distributed upon request. Database deposition of associated metadata and sequence data for each library provides researchers with information to select the most appropriate libraries for further research projects. As a starting point, we have established the Canadian MetaMicroBiome Library (CM(2)BL [1]). The CM(2)BL is a publicly accessible collection of cosmid libraries containing environmental DNA from soils collected from across Canada, spanning multiple biomes. The libraries were constructed such that the cloned DNA can be easily transferred to Gateway® compliant vectors, facilitating functional screening in virtually any surrogate microbial host for which there are available plasmid vectors. The libraries, which we are placing in the public domain, will be distributed upon request without restriction to members of both the

  18. Library support of mobile resources during clinical clerkships.

    Science.gov (United States)

    Stokes, Alice; Light, Jeanene; Haines, Laura L

    2014-01-01

    In response to frequent use of mobile devices among medical students, Dana Medical Library at the University of Vermont provided mobile resource support to medical students preparing for clerkships. The librarians offered group instruction, individual assistance, and an online subject guide. These activities were assessed through evaluations, web statistics, and a survey. Survey questions gathered data on access to mobile devices, use of library-licensed mobile resources, and benefits and barriers to use in the clinical setting. The majority of survey respondents believed access to mobile resources improved their clerkship experience and contributed to comparable educational experiences across clerkship sites. Researchers found that library support affected student perception of the value of mobile resources in the clerkship experience.

  19. Euler European Libraries and Electronic Resources in Mathematical Sciences

    CERN Document Server

    The Euler Project. Karlsruhe

    The European Libraries and Electronic Resources (EULER) Project in Mathematical Sciences provides the EulerService site for searching out "mathematical resources such as books, pre-prints, web-pages, abstracts, proceedings, serials, technical reports preprints) and NetLab (for Internet resources), this outstanding engine is capable of simple, full, and refined searches. It also offers a browse option, which responds to entries in the author, keyword, and title fields. Further information about the Project is provided at the EULER homepage.

  20. The National Resource Library for Science and Technology in Sweden: A Nordic model of cooperating technology libraries

    Science.gov (United States)

    Lagar, Gunnar

    1994-01-01

    The scope of this presentation is to give a state-of-the-art report on the present situation of Nordic technology libraries, to elaborate on a plan for national resource libraries in Sweden, and to share how the Royal Institute of Technology Library in Stockholm (KTHB) has fostered a network of cooperating libraries in order to optimize government funding for the system of resource libraries.

  1. Screening of tissues pooled cDNA library using probes by restricted fragments of BAC positive clones of ovine MHC%以绵羊MHC 区段BAC 克隆酶切片段为探针杂交筛选绵羊混合组织cDNA 文库

    Institute of Scientific and Technical Information of China (English)

    杨小亮; 白大章; 邱巍; 董慧芹; 李大全; 陈芳; 马润林; Hugh T Blair; 高剑峰

    2012-01-01

    Under the premise what we have known bacterial artificial chromosome(BAC) clone sequence information and gene annotation predicted in the Chinese Merino sheep major histocompatibility complex (MHC) region, the digested fragments from 6 BAC clones that were located in the MHC region of the Chinese Merino sheep genome BAC library, which were used to screen the cDNA library using plaque in situ hybridization as probes. The full length of positive cDNA clones (sequences) isolated were completely sequenced, and the sequences obtained were aligned with the corresponding known sequence information and the BAC clones with gene annotation. Meanwhile, the sequence similarity was searched in NCBI Blastn database. This work aimed at verification of accuracy of the gene annotation results and initial analysis of gene (sequence) function. At last, 27 positive cDNA clones (sequences) in total were screened through two runs of hybridization. It was also found that these sequences could be positioned in the corresponding BAC clones, and 25 sequences were located in exon area of the annotated gene. It was verified that 23 sequences had the highest sequence similarity with those in the Bos taurus by searching against the NCBI Blastn database; moreover, the function of these sequences were closely relate to immunology.%在已知中国美利奴羊MHC(Major histocompatibility complex)区段BAC(Bacterial artificial chromosome)克隆序列信息和预测的基因注释前提下,用位于中国美利奴羊基因组BAC 文库MHC 区段的6 个BAC 克隆酶切片段为探针,以噬菌斑原位杂交筛选法筛选中国美利奴羊混合组织cDNA 文库(库库杂交),对分离到的cDNA阳性克隆进行全序列测定,并与相应的已知序列信息和基因注释的BAC 克隆比对以及在NCBI Blastn 数据库中序列相似性检索,旨在验证基因注释结果的准确性和对基因(序列)功能的初步分析.实验中,经过两轮杂交共筛选出27 个cDNA 阳性克隆(序

  2. The European sea bass Dicentrarchus labrax genome puzzle: comparative BAC-mapping and low coverage shotgun sequencing

    Directory of Open Access Journals (Sweden)

    Volckaert Filip AM

    2010-01-01

    Full Text Available Abstract Background Food supply from the ocean is constrained by the shortage of domesticated and selected fish. Development of genomic models of economically important fishes should assist with the removal of this bottleneck. European sea bass Dicentrarchus labrax L. (Moronidae, Perciformes, Teleostei is one of the most important fishes in European marine aquaculture; growing genomic resources put it on its way to serve as an economic model. Results End sequencing of a sea bass genomic BAC-library enabled the comparative mapping of the sea bass genome using the three-spined stickleback Gasterosteus aculeatus genome as a reference. BAC-end sequences (102,690 were aligned to the stickleback genome. The number of mappable BACs was improved using a two-fold coverage WGS dataset of sea bass resulting in a comparative BAC-map covering 87% of stickleback chromosomes with 588 BAC-contigs. The minimum size of 83 contigs covering 50% of the reference was 1.2 Mbp; the largest BAC-contig comprised 8.86 Mbp. More than 22,000 BAC-clones aligned with both ends to the reference genome. Intra-chromosomal rearrangements between sea bass and stickleback were identified. Size distributions of mapped BACs were used to calculate that the genome of sea bass may be only 1.3 fold larger than the 460 Mbp stickleback genome. Conclusions The BAC map is used for sequencing single BACs or BAC-pools covering defined genomic entities by second generation sequencing technologies. Together with the WGS dataset it initiates a sea bass genome sequencing project. This will allow the quantification of polymorphisms through resequencing, which is important for selecting highly performing domesticated fish.

  3. Micronesian Art Historical Research and Library Collection Resources in Micronesia.

    Science.gov (United States)

    Haynes, Douglas

    This paper briefly describes the geographic region and some indigenous artifacts of Micronesia. The state of art historical research in the area and currently available library resources are discussed. Micronesia is comprised of seven island nations peopled by distinctly unique cultural groups. Study of Micronesian art and architecture is…

  4. Student Satisfaction with Electronic Library Resources at Wayne State University

    Science.gov (United States)

    Holley, Robert P.; Powell, Ronald R.

    2004-01-01

    This paper reports the results of a survey of student satisfaction with electronic library resources other than the online catalog at Wayne State University. Undertaken in Fall Term 2000 as a class project for a marketing course, a student team designed, administered, and analyzed a survey of a random sample of students. Almost 40% of the…

  5. Shaping the Curriculum: The Power of a Library's Digital Resources

    Science.gov (United States)

    Kirkwood, Patricia

    2011-01-01

    Researchers were the first adopters of digital resources available through the library. Online journals and databases make finding research articles much easier than when this author started as a librarian more than 20 years ago. Speedier interlibrary loan due to digital delivery means research materials are never far away. Making it easier for…

  6. Videodisc: A New Resource for Library Information Storage and Retrieval.

    Science.gov (United States)

    Sonnemann, Sabine S.

    1984-01-01

    Details a National Library of Canada project to produce a videodisc, show its value as a research tool, and demonstrate its viability as an information storage and retrieval medium. An overview and time sequence of the project, disc contents, resource materials for production, production and postproduction techniques, and project results are…

  7. School Library Journal's 10 Best Digital Resources for 2009

    Science.gov (United States)

    Brisco, Shonda

    2009-01-01

    The author presents 10 best digital resources for 2009. As librarians prepare for the next school year--or as public libraries develop the budget for a new fiscal year--these are the products for children and teens that should be advocated to add to one's digital collection. These include: (1) American Indian Experience; (2) Animoto…

  8. Distribution of Genes and Repetitive Elements in the Diabrotica virgifera virgifera Genome Estimated Using BAC Sequencing

    Directory of Open Access Journals (Sweden)

    Brad S. Coates

    2012-01-01

    Full Text Available Feeding damage caused by the western corn rootworm, Diabrotica virgifera virgifera, is destructive to corn plants in North America and Europe where control remains challenging due to evolution of resistance to chemical and transgenic toxins. A BAC library, DvvBAC1, containing 109,486 clones with 104±34.5 kb inserts was created, which has an ~4.56X genome coverage based upon a 2.58 Gb (2.80 pg flow cytometry-estimated haploid genome size. Paired end sequencing of 1037 BAC inserts produced 1.17 Mb of data (~0.05% genome coverage and indicated ~9.4 and 16.0% of reads encode, respectively, endogenous genes and transposable elements (TEs. Sequencing genes within BAC full inserts demonstrated that TE densities are high within intergenic and intron regions and contribute to the increased gene size. Comparison of homologous genome regions cloned within different BAC clones indicated that TE movement may cause haplotype variation within the inbred strain. The data presented here indicate that the D. virgifera virgifera genome is large in size and contains a high proportion of repetitive sequence. These BAC sequencing methods that are applicable for characterization of genomes prior to sequencing may likely be valuable resources for genome annotation as well as scaffolding.

  9. Human resource management and career planning in a larger library

    Directory of Open Access Journals (Sweden)

    Jelka Gazvoda

    1997-01-01

    Full Text Available Human resource management is presented as a managerial function which is used to develop potential abilities of the employees to achieve organizational goals.Different perception of the employees is essential - people working in the organization are treated as capital and not as an expenditure. In human resource management the most important view of the employees is their potential growth and professional development, training for acquiring new responsibilities and encouragement for innovation. Library management is becoming more and more complex as the result of introducing new technologies. For this reason libraries need well trained people with potentials to modernize library performance and to overcome the conflict between the traditional organizational culture and the requirements of the modem technologically developed environment. The author presents different techniques of active human resource management, which can be used in larger libraries where an appropriate number of employees exists to realize different programmes with. These are programmes for education, staffing,career planning, stimmulation and reward systems, job redefinition and enrichment,and other forms of internal segmentation.

  10. Integrated karyotyping of sorghum by in situ hybridization of landed BACs.

    Science.gov (United States)

    Kim, Jeong-Soon; Childs, Kevin L; Islam-Faridi, M Nurul; Menz, Monica A; Klein, Robert R; Klein, Patricia E; Price, H James; Mullet, John E; Stelly, David M

    2002-04-01

    The reliability of genome analysis and proficiency of genetic manipulation are increased by assignment of linkage groups to specific chromosomes, placement of centromeres, and orientation with respect to telomeres. We have endeavored to establish means to enable these steps in sorghum (Sorghum bicolor (L.) Moench), the genome of which contains ca. 780 Mbp spread across n = 10 chromosomes. Our approach relies on fluorescence in situ hybridization (FISH) and integrated structural genomic resources, including large-insert genomic clones in bacterial artificial chromosome (BAC) libraries. To develop robust FISH probes, we selected sorghum BACs by association with molecular markers that map near the ends of linkage groups, in regions inferred to be high in recombination. Overall, we selected 22 BACs that encompass the 10 linkage groups. As a prelude to development of a multiprobe FISH cocktail, we evaluated BAC-derived probes individually and in small groups. Biotin- and digoxygenin-labeled probes were made directly from the BAC clones and hybridized in situ to chromosomes without using suppressive unlabelled C0t-1 DNA. Based on FISH-signal strength and the relative degree of background signal, we judged 19 BAC-derived probes to be satisfactory. Based on their relative position, and collective association with all 10 linkage groups, we chose 17 of the 19 BACs to develop a 17-locus probe cocktail for dual-color detection. FISH of the cocktail allowed simultaneous identification of all 10 chromosomes. The results indicate that linkage and physical maps of sorghum allow facile selection of BAC clones according to position and FISH-signal quality. This capability will enable development of a high-quality molecular cytogenetic map and an integrated genomics system for sorghum, without need of chromosome flow sorting or microdissection. Moreover, transgeneric FISH experiments suggest that the sorghum system might be applicable to other Gramineae.

  11. Construction of an integrated genetic linkage map for the A genome of Brassica napus using SSR markers derived from sequenced BACs in B. rapa

    Directory of Open Access Journals (Sweden)

    King Graham J

    2010-10-01

    Full Text Available Abstract Background The Multinational Brassica rapa Genome Sequencing Project (BrGSP has developed valuable genomic resources, including BAC libraries, BAC-end sequences, genetic and physical maps, and seed BAC sequences for Brassica rapa. An integrated linkage map between the amphidiploid B. napus and diploid B. rapa will facilitate the rapid transfer of these valuable resources from B. rapa to B. napus (Oilseed rape, Canola. Results In this study, we identified over 23,000 simple sequence repeats (SSRs from 536 sequenced BACs. 890 SSR markers (designated as BrGMS were developed and used for the construction of an integrated linkage map for the A genome in B. rapa and B. napus. Two hundred and nineteen BrGMS markers were integrated to an existing B. napus linkage map (BnaNZDH. Among these mapped BrGMS markers, 168 were only distributed on the A genome linkage groups (LGs, 18 distrubuted both on the A and C genome LGs, and 33 only distributed on the C genome LGs. Most of the A genome LGs in B. napus were collinear with the homoeologous LGs in B. rapa, although minor inversions or rearrangements occurred on A2 and A9. The mapping of these BAC-specific SSR markers enabled assignment of 161 sequenced B. rapa BACs, as well as the associated BAC contigs to the A genome LGs of B. napus. Conclusion The genetic mapping of SSR markers derived from sequenced BACs in B. rapa enabled direct links to be established between the B. napus linkage map and a B. rapa physical map, and thus the assignment of B. rapa BACs and the associated BAC contigs to the B. napus linkage map. This integrated genetic linkage map will facilitate exploitation of the B. rapa annotated genomic resources for gene tagging and map-based cloning in B. napus, and for comparative analysis of the A genome within Brassica species.

  12. RESOURCES CONSUMPTION MANAGEMENT AT THE NATIONAL LIBRARY OF IRAN

    Directory of Open Access Journals (Sweden)

    K. Naddafi, J. Nouri, R. Nabizadeh, N. Shahbod

    2007-07-01

    Full Text Available This paper studies the various resources (water, energy, paper as well as other wastes consumption management at the National Library of Iran in Tehran. After setting the targets and specifying the correct management framework of resources consumption through the green management schedule, the resources analysis in consumption pattern in National Library of Iran was set up. Some attempt plans were performed to find out management solutions to the related issues. For the achievement of this aim, the first step was considered the study of general conditions of the Library through several visits, completing the checklists and questionnaires and then gathering the necessary information and analyzing them. The results of the fuel consumption in transportations related to the National Library showed that the major pollutants produced by the transportation were CO2, CO, NOx, and hydrocarbons. The rate of CO2 had a maximum level of 904 Kg and CO was 6 Kg, NOx was 2 Kg and hydrocarbons were 0.58 Kg. Among the equipments, which use energy in the Library, refrigerators produce the levels of CO2, SO2 and NOx .The total level of CO2, SO2 and NOx produced by all the equipments were 1140.32 Kg, 3.9072 kg and 2.886 Kg respectively. The results of water investigations showed that the total water consumption was 45459 L/year and the greater part of it was related to irrigation of the garden in the courtyard which was equal to 32471 L/year. Regarding to the wastes, the results of 30 times sampling showed the highest level to be related to aluminum and paper and a computer analysis revealed these results in the rates of 19745 Kg/year of CO2, 77.54 Kg/year, SO2 57.4Kg/year NOx and particulate matter was 2.92 Kg/year. Following the investigations carried out in management, executive solutions were suggested which led to the conclusion that the Green Management System should be established at this organization which should result in optimum consumption of resources

  13. Construction and Analysis of Siberian Tiger Bacterial Artificial Chromosome Library with Approximately 6.5-Fold Genome Equivalent Coverage

    Directory of Open Access Journals (Sweden)

    Changqing Liu

    2014-03-01

    Full Text Available Bacterial artificial chromosome (BAC libraries are extremely valuable for the genome-wide genetic dissection of complex organisms. The Siberian tiger, one of the most well-known wild primitive carnivores in China, is an endangered animal. In order to promote research on its genome, a high-redundancy BAC library of the Siberian tiger was constructed and characterized. The library is divided into two sub-libraries prepared from blood cells and two sub-libraries prepared from fibroblasts. This BAC library contains 153,600 individually archived clones; for PCR-based screening of the library, BACs were placed into 40 superpools of 10 × 384-deep well microplates. The average insert size of BAC clones was estimated to be 116.5 kb, representing approximately 6.46 genome equivalents of the haploid genome and affording a 98.86% statistical probability of obtaining at least one clone containing a unique DNA sequence. Screening the library with 19 microsatellite markers and a SRY sequence revealed that each of these markers were present in the library; the average number of positive clones per marker was 6.74 (range 2 to 12, consistent with 6.46 coverage of the tiger genome. Additionally, we identified 72 microsatellite markers that could potentially be used as genetic markers. This BAC library will serve as a valuable resource for physical mapping, comparative genomic study and large-scale genome sequencing in the tiger.

  14. Construction and analysis of Siberian tiger bacterial artificial chromosome library with approximately 6.5-fold genome equivalent coverage.

    Science.gov (United States)

    Liu, Changqing; Bai, Chunyu; Guo, Yu; Liu, Dan; Lu, Taofeng; Li, Xiangchen; Ma, Jianzhang; Ma, Yuehui; Guan, Weijun

    2014-03-07

    Bacterial artificial chromosome (BAC) libraries are extremely valuable for the genome-wide genetic dissection of complex organisms. The Siberian tiger, one of the most well-known wild primitive carnivores in China, is an endangered animal. In order to promote research on its genome, a high-redundancy BAC library of the Siberian tiger was constructed and characterized. The library is divided into two sub-libraries prepared from blood cells and two sub-libraries prepared from fibroblasts. This BAC library contains 153,600 individually archived clones; for PCR-based screening of the library, BACs were placed into 40 superpools of 10 × 384-deep well microplates. The average insert size of BAC clones was estimated to be 116.5 kb, representing approximately 6.46 genome equivalents of the haploid genome and affording a 98.86% statistical probability of obtaining at least one clone containing a unique DNA sequence. Screening the library with 19 microsatellite markers and a SRY sequence revealed that each of these markers were present in the library; the average number of positive clones per marker was 6.74 (range 2 to 12), consistent with 6.46 coverage of the tiger genome. Additionally, we identified 72 microsatellite markers that could potentially be used as genetic markers. This BAC library will serve as a valuable resource for physical mapping, comparative genomic study and large-scale genome sequencing in the tiger.

  15. A BAC-based physical map of the Drosophila buzzatii genome

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez, Josefa; Nefedov, Michael; Bosdet, Ian; Casals, Ferran; Calvete, Oriol; Delprat, Alejandra; Shin, Heesun; Chiu, Readman; Mathewson, Carrie; Wye, Natasja; Hoskins, Roger A.; Schein, JacquelineE.; de Jong, Pieter; Ruiz, Alfredo

    2005-03-18

    Large-insert genomic libraries facilitate cloning of large genomic regions, allow the construction of clone-based physical maps and provide useful resources for sequencing entire genomes. Drosophilabuzzatii is a representative species of the repleta group in the Drosophila subgenus, which is being widely used as a model in studies of genome evolution, ecological adaptation and speciation. We constructed a Bacterial Artificial Chromosome (BAC) genomic library of D. buzzatii using the shuttle vector pTARBAC2.1. The library comprises 18,353 clones with an average insert size of 152 kb and a {approx}18X expected representation of the D. buzzatii euchromatic genome. We screened the entire library with six euchromatic gene probes and estimated the actual genome representation to be {approx}23X. In addition, we fingerprinted by restriction digestion and agarose gel electrophoresis a sample of 9,555 clones, and assembled them using Finger Printed Contigs (FPC) software and manual editing into 345 contigs (mean of 26 clones per contig) and 670singletons. Finally, we anchored 181 large contigs (containing 7,788clones) to the D. buzzatii salivary gland polytene chromosomes by in situ hybridization of 427 representative clones. The BAC library and a database with all the information regarding the high coverage BAC-based physical map described in this paper are available to the research community.

  16. An anchored framework BAC map of mouse chromosome 11 assembled using multiplex oligonucleotide hybridization.

    Science.gov (United States)

    Cai, W W; Reneker, J; Chow, C W; Vaishnav, M; Bradley, A

    1998-12-15

    Despite abundant library resources for many organisms, physical mapping of these organisms has been seriously limited due to lack of efficient library screening techniques. We have developed a highly efficient strategy for large-scale screening of genomic libraries based on multiplex oligonucleotide hybridization on high-density genomic filters. We have applied this strategy to generate a bacterial artificial chromosome (BAC) anchored map of mouse chromosome 11. Using the MIT mouse SSLP data, 320 pairs of oligonucleotide probes were designed with an "overgo" computer program that selects new primer sequences that avoid the microsatellite repeat. BACs identified by these probes are automatically anchored to the chromosome. Ninety-two percent of the probes identified positive clones from a 5.9-fold coverage mouse BAC library with an average of 7 positive clones per marker. An average of 4.2 clones was confirmed for 204 markers by PCR. Our data show that a large number of clones can be efficiently isolated from a large genomic library using this strategy with minimal effort. This strategy will have wide application for large-scale mapping and sequencing of human and other large genomes.

  17. A Study on Developing Evaluation Criteria for Electronic Resources in Evaluation Indicators of Libraries

    Science.gov (United States)

    Noh, Younghee

    2010-01-01

    This study aimed to improve the current state of electronic resource evaluation in libraries. While the use of Web DB, e-book, e-journal, and other e-resources such as CD-ROM, DVD, and micro materials is increasing in libraries, their use is not comprehensively factored into the general evaluation of libraries and may diminish the reliability of…

  18. Sequencing, annotation and comparative analysis of nine BACs of giant panda (Ailuropoda melanoleuca).

    Science.gov (United States)

    Zheng, Yang; Cai, Jing; Li, JianWen; Li, Bo; Lin, RunMao; Tian, Feng; Wang, XiaoLing; Wang, Jun

    2010-01-01

    A 10-fold BAC library for giant panda was constructed and nine BACs were selected to generate finish sequences. These BACs could be used as a validation resource for the de novo assembly accuracy of the whole genome shotgun sequencing reads of giant panda newly generated by the Illumina GA sequencing technology. Complete sanger sequencing, assembly, annotation and comparative analysis were carried out on the selected BACs of a joint length 878 kb. Homologue search and de novo prediction methods were used to annotate genes and repeats. Twelve protein coding genes were predicted, seven of which could be functionally annotated. The seven genes have an average gene size of about 41 kb, an average coding size of about 1.2 kb and an average exon number of 6 per gene. Besides, seven tRNA genes were found. About 27 percent of the BAC sequence is composed of repeats. A phylogenetic tree was constructed using neighbor-join algorithm across five species, including giant panda, human, dog, cat and mouse, which reconfirms dog as the most related species to giant panda. Our results provide detailed sequence and structure information for new genes and repeats of giant panda, which will be helpful for further studies on the giant panda.

  19. Sequencing,annotation and comparative analysis of nine BACs of the giant panda(Ailuropoda melanoleuca)

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    A 10-fold BAC library for the giant panda was constructed and nine BACs were selected to generate finish sequences.These BACs could be used as a validation resource for the de novo assembly accuracy of the whole genome shotgun sequencing reads of the giant panda newly generated by Illumina GA sequencing technology.Complete Sanger sequencing,assembly,annotation and comparative analysis were carried out on the selected BACs of a joint length 878 kb.Homologue search and de novo prediction methods were used to annotate genes and repeats.Twelve protein coding genes were predicted,seven of which could be functionally annotated.The seven genes have an average gene size of about 41 kb,an average coding size of about 1.2 kb and an average exon number of 6 per gene.Besides,seven tRNA genes were found.About 27 percent of the BAC sequence is composed of repeats.A phylogenetic tree was constructed using a neighbor-join algorithm across five species,including the giant panda,human,dog,cat and mouse,which reconfirms dog as the most closely related species to the giant panda.Our results provide detailed sequence and structure information for new genes and repeats of the giant panda,which will be helpful for further studies about the giant panda.

  20. Analysis of BAC end sequences in oak, a keystone forest tree species, providing insight into the composition of its genome

    Directory of Open Access Journals (Sweden)

    Le Provost Grégoire

    2011-06-01

    Full Text Available Abstract Background One of the key goals of oak genomics research is to identify genes of adaptive significance. This information may help to improve the conservation of adaptive genetic variation and the management of forests to increase their health and productivity. Deep-coverage large-insert genomic libraries are a crucial tool for attaining this objective. We report herein the construction of a BAC library for Quercus robur, its characterization and an analysis of BAC end sequences. Results The EcoRI library generated consisted of 92,160 clones, 7% of which had no insert. Levels of chloroplast and mitochondrial contamination were below 3% and 1%, respectively. Mean clone insert size was estimated at 135 kb. The library represents 12 haploid genome equivalents and, the likelihood of finding a particular oak sequence of interest is greater than 99%. Genome coverage was confirmed by PCR screening of the library with 60 unique genetic loci sampled from the genetic linkage map. In total, about 20,000 high-quality BAC end sequences (BESs were generated by sequencing 15,000 clones. Roughly 5.88% of the combined BAC end sequence length corresponded to known retroelements while ab initio repeat detection methods identified 41 additional repeats. Collectively, characterized and novel repeats account for roughly 8.94% of the genome. Further analysis of the BESs revealed 1,823 putative genes suggesting at least 29,340 genes in the oak genome. BESs were aligned with the genome sequences of Arabidopsis thaliana, Vitis vinifera and Populus trichocarpa. One putative collinear microsyntenic region encoding an alcohol acyl transferase protein was observed between oak and chromosome 2 of V. vinifera. Conclusions This BAC library provides a new resource for genomic studies, including SSR marker development, physical mapping, comparative genomics and genome sequencing. BES analysis provided insight into the structure of the oak genome. These sequences will be

  1. Construction and Preliminary Characterization Analysis of Wuzhishan Miniature Pig Bacterial Artificial Chromosome Library with Approximately 8-Fold Genome Equivalent Coverage

    Directory of Open Access Journals (Sweden)

    Changqing Liu

    2013-01-01

    Full Text Available Bacterial artificial chromosome (BAC libraries have been invaluable tools for the genome-wide genetic dissection of complex organisms. Here, we report the construction and characterization of a high-redundancy BAC library from a very valuable pig breed in China, Wuzhishan miniature pig (Sus scrofa, using its blood cells and fibroblasts, respectively. The library contains approximately 153,600 clones ordered in 40 superpools of 10 × 384-deep well microplates. The average insert size of BAC clones was estimated to be 152.3 kb, representing approximately 7.68 genome equivalents of the porcine haploid genome and a 99.93% statistical probability of obtaining at least one clone containing a unique DNA sequence in the library. 19 pairs of microsatellite marker primers covering porcine chromosomes were used for screening the BAC library, which showed that each of these markers was positive in the library; the positive clone number was 2 to 9, and the average number was 7.89, which was consistent with 7.68-fold coverage of the porcine genome. And there were no significant differences of genomic BAC library from blood cells and fibroblast cells. Therefore, we identified 19 microsatellite markers that could potentially be used as genetic markers. As a result, this BAC library will serve as a valuable resource for gene identification, physical mapping, and comparative genomics and large-scale genome sequencing in the porcine.

  2. Toward a molecular cytogenetic map for cultivated sunflower (Helianthus annuus L.) by landed BAC/BIBAC clones.

    Science.gov (United States)

    Feng, Jiuhuan; Liu, Zhao; Cai, Xiwen; Jan, Chao-Chien

    2013-01-01

    Conventional karyotypes and various genetic linkage maps have been established in sunflower (Helianthus annuus L., 2n = 34). However, the relationship between linkage groups and individual chromosomes of sunflower remains unknown and has considerable relevance for the sunflower research community. Recently, a set of linkage group-specific bacterial /binary bacterial artificial chromosome (BAC/BIBAC) clones was identified from two complementary BAC and BIBAC libraries constructed for cultivated sunflower cv. HA89. In the present study, we used these linkage group-specific clones (~100 kb in size) as probes to in situ hybridize to HA89 mitotic chromosomes at metaphase using the BAC-fluorescence in situ hybridization (FISH) technique. Because a characteristic of the sunflower genome is the abundance of repetitive DNA sequences, a high ratio of blocking DNA to probe DNA was applied to hybridization reactions to minimize the background noise. As a result, all sunflower chromosomes were anchored by one or two BAC/BIBAC clones with specific FISH signals. FISH analysis based on tandem repetitive sequences, such as rRNA genes, has been previously reported; however, the BAC-FISH technique developed here using restriction fragment length polymorphism (RFLP)-derived BAC/BIBAC clones as probes to apply genome-wide analysis is new for sunflower. As chromosome-specific cytogenetic markers, the selected BAC/BIBAC clones that encompass the 17 linkage groups provide a valuable tool for identifying sunflower cytogenetic stocks (such as trisomics) and tracking alien chromosomes in interspecific crosses. This work also demonstrates the potential of using a large-insert DNA library for the development of molecular cytogenetic resources.

  3. Recombineering linear BACs.

    Science.gov (United States)

    Chen, Qingwen; Narayanan, Kumaran

    2015-01-01

    Recombineering is a powerful genetic engineering technique based on homologous recombination that can be used to accurately modify DNA independent of its sequence or size. One novel application of recombineering is the assembly of linear BACs in E. coli that can replicate autonomously as linear plasmids. A circular BAC is inserted with a short telomeric sequence from phage N15, which is subsequently cut and rejoined by the phage protelomerase enzyme to generate a linear BAC with terminal hairpin telomeres. Telomere-capped linear BACs are protected against exonuclease attack both in vitro and in vivo in E. coli cells and can replicate stably. Here we describe step-by-step protocols to linearize any BAC clone by recombineering, including inserting and screening for presence of the N15 telomeric sequence, linearizing BACs in vivo in E. coli, extracting linear BACs, and verifying the presence of hairpin telomere structures. Linear BACs may be useful for functional expression of genomic loci in cells, maintenance of linear viral genomes in their natural conformation, and for constructing innovative artificial chromosome structures for applications in mammalian and plant cells.

  4. 牦牛瘤胃元基因组文库中木聚糖酶基因的分析%Analysis of xylanases derived from the metagenomic BAC clone library of yak rumen

    Institute of Scientific and Technical Information of China (English)

    王敏; 陈富荣; 张山; 朱雅新; 东秀珠; 黄力; 田瑞华; 董志扬; 戴欣

    2011-01-01

    [ Objective ] The diversity of rumen microbial xylanases and their degradation characteristics were studied and the resources of new xylanases genes and enzymes were provided. [ Methods ] According to the result of the high-throughput sequencing of the rumen microbial metagenome bacterial artificial chromosome ( B AC ) clones library, the diversity of xylanase genes was analyzed and screened. Then one xylanase and its downstream xylosidase gene screened was cloned and expressed in Escherichia coli. The enzyme characterization of the recombinant xylanase and xylosidase and their synergistic effect were studied. [Results] All 14 xylanases screened from the library belong to GH10 family proteins. These xylanases shared the amino acid sequence similarity between 20. 5% and 91. 3% . Intriguingly, 7 xylanase genes in different contigs were found to be followed by xylosidase gene. The specific enzyme activity of the xylanase (Xyn32) was 1.98 U/mg and no ferulic acid esterase activity was detected. The specific enzyme activity of the coupled xylosidase (Xyl33) was 0.07 IU/mg, and xylosidase ( Xyl33 ) also displayed the activity of arabinofuranosidase. In addition, the in vitro experiment confirmed the synergistic effect between the coupled xylanase and xylosidase.%[目的]了解牦牛瘤胃微生物木聚糖酶多样性及其降解特征,为木聚糖降解提供新的基因资源.[方法]根据对已构建的瘤胃微生物元基因组细菌人工染色体( BAC)克隆文库高通量测序结果的注释,筛选其中编码木聚糖酶的基因并进行多样性分析;对其中一个木聚糖酶基因及其连锁的木糖苷酶基因进行克隆表达和酶学性质表征,分析其协同作用.[结果]共筛选到14个木聚糖酶基因,均编码GH1O家族木聚糖酶,其氨基酸序列之间的相似性为20.5% -91.3%;其中7个木聚糖酶基因所在的不同的DNA片段(contig)上存在木糖苷酶基因,编码的木糖苷酶属于GH43或GH3糖苷水解酶家

  5. Library Resource-Sharing in the Network-Centric World.

    Science.gov (United States)

    McGee, Rob

    This paper discusses changes in services, technology, and organization as libraries prepare to enter the "network-centric library world." Part 1 addresses the transition from the analog era to the digital age, and the convergence of libraries and education, including opportunities for library leadership in Internet access, digital…

  6. The Study of Analytical Model of Library Electronic Resources Usage-A Case of Medical Electronic Resources

    OpenAIRE

    Chung-Yen Yu; Jiann-Cherng Shieh

    2014-01-01

    With the advents of internet, the importance of electronic resources is growing. Due to the increasing expensiveness of electronic resources, university libraries normally received budgets from parent institutions annually. They necessarily applied effective and systematic methods for decision making in electronic resources purchase or re-subscription. However, there are some difficulties in practices: First of all, libraries are unable to receive user records; second, the COUNTER statistics ...

  7. Preservation and Conservation of Information Resources in the University of Zambia Library

    Science.gov (United States)

    Kanyengo, Christine Wamunyima

    2009-01-01

    Preservation and conservation of library materials is an important aspect of library and information management. Their importance and necessity are more paramount in countries where resources are limited and libraries need to balance them with the needs of an ever increasing number of students hoping to use them. This article reports on the…

  8. An Integrated Library Platform: Wales' Approach to Delivering Digital Information and Resources Nationally

    Science.gov (United States)

    Bevan, Paul; Tyler, Alyson

    2009-01-01

    Purpose: This paper aims to outline the developments and strategies employed to supply online library services in Wales through a national platform: library.wales.org These services include: the "Cat Cymru" cross-catalogue search, centrally procured subscription resources and local library microsites. Design/methodology/approach: The…

  9. Discipline, Availability of Electronic Resources and the Use of Finnish National Electronic Library-- FinELib

    Science.gov (United States)

    Törmä, Sanna; Vakkari, Pertti

    2004-01-01

    This study elaborated relations between digital library use by university faculty, users' discipline and the availability of key resources in the Finnish National Electronic Library (FinELib), Finnish national digital library, by using nationwide representative survey data. The results show that the perceived availability of key electronic…

  10. Hacking Blackboard: Customizing Access to Library Resources through the Blackboard Course Management System

    Science.gov (United States)

    Kellam, Lynda M.; Cox, Richard; Winkler, Hannah

    2009-01-01

    Academic libraries have long been trying to gain access to users through their favorite online spaces, such as social networking sites. In this article a project of the University of North Carolina at Greensboro's University Libraries, which integrated library resources with the campus course management system, Blackboard, is detailed. The main…

  11. The Impact of Library Resources and Services on the Scholarly Activity of Medical Faculty and Residents.

    Science.gov (United States)

    Quesenberry, Alexandria C; Oelschlegel, Sandy; Earl, Martha; Leonard, Kelsey; Vaughn, Cynthia J

    2016-01-01

    Librarians at an academic medical center library gathered data to determine if library services and resources impacted scholarly activity. A survey was developed and sent out to faculty and residents asking how they used the library during scholarly activity. Sixty-five faculty members and residents responded to the survey. The majority of respondents involved with scholarly activity use the library's services and resources. PubMed is the most frequently used database. The positive results show the library impacts the scholarly activity of medical faculty and residents.

  12. Creating new library services through collaboration with resident groups : Aimimg at human resource development and information literacy education in ways only libraries can do : Study on activities of an NPO called Ueda Library Club

    Science.gov (United States)

    Morita, Utako

    Creating new library services through collaboration with resident groups : Aimimg at human resource development and information literacy education in ways only libraries can do : Study on activities of an NPO called Ueda Library Club

  13. Genomic tools development for Aquilegia: construction of a BAC-based physical map

    Directory of Open Access Journals (Sweden)

    Hodges Scott A

    2010-11-01

    Full Text Available Abstract Background The genus Aquilegia, consisting of approximately 70 taxa, is a member of the basal eudicot lineage, Ranuculales, which is evolutionarily intermediate between monocots and core eudicots, and represents a relatively unstudied clade in the angiosperm phylogenetic tree that bridges the gap between these two major plant groups. Aquilegia species are closely related and their distribution covers highly diverse habitats. These provide rich resources to better understand the genetic basis of adaptation to different pollinators and habitats that in turn leads to rapid speciation. To gain insights into the genome structure and facilitate gene identification, comparative genomics and whole-genome shotgun sequencing assembly, BAC-based genomics resources are of crucial importance. Results BAC-based genomic resources, including two BAC libraries, a physical map with anchored markers and BAC end sequences, were established from A. formosa. The physical map was composed of a total of 50,155 BAC clones in 832 contigs and 3939 singletons, covering 21X genome equivalents. These contigs spanned a physical length of 689.8 Mb (~2.3X of the genome suggesting the complex heterozygosity of the genome. A set of 197 markers was developed from ESTs induced by drought-stress, or involved in anthocyanin biosynthesis or floral development, and was integrated into the physical map. Among these were 87 genetically mapped markers that anchored 54 contigs, spanning 76.4 Mb (25.5% across the genome. Analysis of a selection of 12,086 BAC end sequences (BESs from the minimal tiling path (MTP allowed a preview of the Aquilegia genome organization, including identification of transposable elements, simple sequence repeats and gene content. Common repetitive elements previously reported in both monocots and core eudicots were identified in Aquilegia suggesting the value of this genome in connecting the two major plant clades. Comparison with sequenced plant genomes

  14. Construction and analysis of a BAC library of Streptomyces avermitilis genome%阿维链霉菌BAC文库的构建及分析

    Institute of Scientific and Technical Information of China (English)

    刘家栋; 王革娇; 罗美中

    2016-01-01

    为进一步阐明阿维菌素的生物合成路径和调控途径,提高阿维菌素的产量,采用Bam H I限制性内切酶酶解阿维链霉菌基因组DNA的方法,构建了阿维链霉菌的细菌人工染色体(BAC)文库.该文库共包含2 304个克隆,平均插入片段为101 kb,空载率约9%,覆盖了该菌基因组的25.9倍.该文库的成功构建可以为其他微生物尤其是基因组具有磷硫酰化修饰的微生物的文库构建提供参考.

  15. Discipline, availability of electronic resources and the use of Finnish National Electronic Library - FinELib

    Directory of Open Access Journals (Sweden)

    Sanna Torma

    2004-01-01

    Full Text Available This study elaborated relations between digital library use by university faculty, users' discipline and the availability of key resources in the Finnish National Electronic Library (FinELib, Finnish national digital library, by using nationwide representative survey data. The results show that the perceived availability of key electronic resources by researchers in FinELib was a stronger predictor of the frequency and purpose of use of its services than users' discipline. Regardless of discipline a good perceived provision of central resources led to a more frequent use of FinELib. The satisfaction with the services did not vary with the discipline, but with the perceived availability of resources.

  16. Users' Satisfaction with Library Information Resources and Services: A Case Study College of Health Sciences Library Niger Delta University, Amassoma, Nigeria

    Science.gov (United States)

    Tiemo, Pereware Aghwotu; Ateboh, Benedict Alaowei

    2016-01-01

    This study investigated users' satisfaction with library information resources and services at the College of Health Sciences (CHS) library Niger Delta University, Nigeria. The objective was to determine the level of users satisfaction with library information resources and services. 2 (two) research questions were formulated to guide the study.…

  17. An Evaluation of the Accessibility of E-resources from Theological Library Websites

    Directory of Open Access Journals (Sweden)

    Kate L. Ganski

    2008-06-01

    Full Text Available This study examines the accessibility of e-resources from the websites of theological libraries to discover if theological libraries are providing digital access to e-journals, recommended religious Internet resources, and digital libraries. Qualitative content analysis was used to evaluate the placement and terminology of e-resources on twenty-five theological library websites of faith-based institutions with primarily graduate/professional students. Findings revealed slightly more than half of these websites make e-resources available and are easily accessible through a quick navigational pathway. Terminology used was found to be diverse and varied. These findings suggest that theological library websites are attempting to meet the digital needs of their students. Further study is recommended to understand the impact, if any, these findings may have on the digital needs of theological students.

  18. Legal Resources Information System for Information Agencies of Specialized Libraries

    CERN Document Server

    Nguyen, Phuc V

    2011-01-01

    In recent years, the rapid development of information technology and communication has a strong impact to industry information - the library. The mission of the industry when in fact the great social place to see the library as knowledge management. Vietnam is in the process of building the rule of law socialist orientation and improves the legal system. So in the current development process, the law library plays an important role in the retention, dissemination and provision of legal information service of legislative, executive and judiciary, particularly especially research, teaching and learning of law school. But the response of the legal information library information agencies remains limited compared to the increasing demand of users.

  19. To get or not to get: the KAUST library e-resources acquisition experience

    KAUST Repository

    Ramli, Rindra M.

    2014-06-01

    In the challenging times of budget cuts and reviews, libraries are faced with issues, among others, such as justifying acquisition, negotiating deals and reviewing current subscriptions (pertaining to electronic resources). With the rapid increase in growth of electronic resources, libraries have to continuously assess their acquisition models and policies to constantly ensure that they are balancing their budget and users’ needs as well. This paper highlights the role played by Technical and IT department of King Abdullah University of Science and Technology (KAUST) library (Saudi Arabia) in acquiring the electronic resources (electronic books, electronic journals, databases, image and reference) for the community. It will describe the processes during the early days when KAUST library was inaugurated; how electronic resources were acquired and what went through during those days. The paper will elaborate further how the acquisition model has evolved and the various important roles played by the library staff in ensuring that acquisitions/subscriptions are justified, within the budget and provides ROI for the library. King Abdullah University is a graduate research university which opened in September 2009 with its first cohort of 800 graduate students (25% female) taught by 100 faculties. The focus of study and research in the university are: Mathematics and Computer Science, Physical Sciences and Life Sciences. The university library started with 10 staff. The library has a “state-of-the-art learning and information resource center supporting graduate education and advanced scientific research” (KAUST, 2010).

  20. The Best Cataloger is a Frustrated Library User: Cataloging Failure and the Underutilization of Library Resources

    Directory of Open Access Journals (Sweden)

    John W. Thompson

    2015-08-01

    Full Text Available This essay points out that inconsistencies in the assignment of subject headings and call number can lead to failure to retrieve relevant materials from our libraries. Today it is frequently asserted that bibliographic records cataloged by the Library of Congress or other approved libraries will not require review or editing in our local libraries. This paper provides clear, but by no means unique examples of “cataloging failure” and explains the implications of a policy to add unedited bibliographic records (from vendors such as OCLC to our library catalogs. The result is the omission of otherwise relevant titles from fairly routine searches.

  1. [Primary care resources available in digital libraries in Spanish Autonomous Regions].

    Science.gov (United States)

    Juan-Quilis, Verónica

    2013-03-01

    The Statement by the Spanish Society of Family and Community Medicine (SemFYC) on access to scientific information, highlights the need for providing digital libraries with certain resources in Autonomous Regions. The primary goal is to study the evidence-based medicine (EBM) coverage that SemFYC recommends regional virtual libraries. The regional health virtual libraries were identified and the access provided to health professionals, Internet presence, remote access and resources were studied. The results suggest there is ample coverage in 8 Autonomous Regions. At the top of the list was, Health Sciences Virtual Library of Navarre, the Balearic Islands Health Sciences Virtual Library, and Virtual Library of the Andalusian Public Health System. The present study needs to be extended to the other biomedical sciences, in order to obtain more accurate results.

  2. Public Library Use of Free E-Resources

    Science.gov (United States)

    Hill, Heather; Bossaller, Jenny

    2013-01-01

    This article describes a multi-method research project examining the use of various freely available online collections and projects, such as Project Gutenberg, the Internet Archive, and Creative Commons-licensed ebooks, by public libraries. This research begins with the questions: what are libraries doing with freely available materials? Are…

  3. A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni

    Directory of Open Access Journals (Sweden)

    Coen M Adema

    2006-10-01

    Full Text Available To provide a novel resource for analysis of the genome of Biomphalaria glabrata, members of the international Biomphalaria glabrata Genome Initiative (biology.unm.edu/biomphalaria-genome.html, working with the Arizona Genomics Institute (AGI and supported by the National Human Genome Research Institute (NHGRI, produced a high quality bacterial artificial chromosome (BAC library. The BB02 strain B. glabrata, a field isolate (Belo Horizonte, Minas Gerais, Brasil that is susceptible to several strains of Schistosoma mansoni, was selfed for two generations to reduce haplotype diversity in the offspring. High molecular weight DNA was isolated from ovotestes of 40 snails, partially digested with HindIII, and ligated into pAGIBAC1 vector. The resulting B. glabrata BAC library (BG_BBa consists of 61824 clones (136.3 kb average insert size and provides 9.05 × coverage of the 931 Mb genome. Probing with single/low copy number genes from B. glabrata and fingerprinting of selected BAC clones indicated that the BAC library sufficiently represents the gene complement. BAC end sequence data (514 reads, 299860 nt indicated that the genome of B. glabrata contains ~ 63% AT, and disclosed several novel genes, transposable elements, and groups of high frequency sequence elements. This BG_BBa BAC library, available from AGI at cost to the research community, gains in relevance because BB02 strain B. glabrata is targeted whole genome sequencing by NHGRI.

  4. Developing as new search engine and browser for libraries to search and organize the World Wide Web library resources

    OpenAIRE

    Sreenivasulu, V.

    2000-01-01

    Internet Granthalaya urges world wide advocates and targets at the task of creating a new search engine and dedicated browseer. Internet Granthalaya may be the ultimate search engine exclusively dedicated for every library use to search and organize the world wide web libary resources

  5. Enhancing Teaching and Learning: Libraries and Open Educational Resources in the Classroom

    Science.gov (United States)

    Davis, Erin; Cochran, Dory; Fagerheim, Britt; Thoms, Becky

    2016-01-01

    Academic libraries continually adjust services to adapt to the ever-changing landscape in higher education. In response to the broken textbook market, libraries are becoming actively involved in the open educational resources (OER) movement. Although there is not a formal program in place, librarians at Utah State University explored a…

  6. The small and rural academic library leveraging resources and overcoming limitations

    CERN Document Server

    Davis Kendrick, Kaetrena

    2016-01-01

    Through the use of case studies, research, and practical interviews, The Small or Rural Academic Library: Leveraging Resources and Overcoming Limitations explores how academic librarians in such environments can keep pace with, create, and improve modern library practices and services, network with colleagues, and access continuing education and professional development opportunities.

  7. E-Resources, Services and User Surveys in Tsinghua University Library

    Science.gov (United States)

    Min, Shao; Yi, Yang

    2010-01-01

    Purpose: The paper aims to provide an overview of the e-resources and services in Tsinghua University Library, Beijing, and describe five campus-wide user surveys. It states how these user surveys play an important role in service quality enhancement. Design/methodology/approach: Following a brief history of Tsinghua University Library, a summary…

  8. Assessment of Data Management Services at New England Region Resource Libraries

    Directory of Open Access Journals (Sweden)

    Julie Goldman

    2015-07-01

    Full Text Available Objective: To understand how New England medical libraries are addressing scientific research data management and providing services to their communities. Setting: The National Network of Libraries of Medicine, New England Region (NN/LM NER contains 17 Resource Libraries. The University of Massachusetts Medical School serves as the New England Regional Medical Library (RML. Sixteen of the NER Resource Libraries completed this survey. Methods: A 40-question online survey assessed libraries’ services and programs for providing research data management education and support. Libraries shared their current plans and institutional challenges associated with developing data services. Results: This study shows few NER Resource Libraries currently integrate scientific research data management into their services and programs, and highlights the region’s use of resources provided by the NN/LM NER RML at the University of Massachusetts Medical School. Conclusions: Understanding the types of data services being delivered at NER libraries helps to inform the NN/LM NER about the eScience learning needs of New England medical librarians and helps in the planning of professional development programs that foster effective biomedical research data services.

  9. Technological Change in the Workplace: A Statewide Survey of Community College Library and Learning Resources Personnel.

    Science.gov (United States)

    Poole, Carolyn E.; Denny, Emmett

    2001-01-01

    Discussion of the effects of technostress on library personnel focuses on an investigation that examined how employees in Florida community college libraries and learning resources centers are dealing with technological change in their work environment. Considers implications for planning and implementing technological change and includes…

  10. Developing Awareness and Use of Library Resources in Undergraduate Sociology: A Sample Assignment.

    Science.gov (United States)

    Abowitz, Deborah A.

    1994-01-01

    Asserts that one of the increasingly critical skills that undergraduates must master is electronic information retrieval. Maintains that sociologists can readily integrate library instruction into undergraduate courses and help develop student awareness of and ability to use the ever-growing body of library resources and information. (CFR)

  11. Organizational Schemes of Information Resources in Top 50 Academic Business Library Websites

    Science.gov (United States)

    Kim, Soojung; DeCoster, Elizabeth

    2011-01-01

    This paper analyzes the organizational schemes of information resources found in top 50 academic business library websites through content analysis and discusses the development and evaluation of the identified schemes.

  12. Quality control of the sheep bacterial artificial chromosome library, CHORI-243

    Directory of Open Access Journals (Sweden)

    Kirkness Ewen F

    2010-12-01

    Full Text Available Abstract Background The sheep CHORI-243 bacterial artificial chromosome (BAC library is being used in the construction of the virtual sheep genome, the sequencing and construction of the actual sheep genome assembly and as a source of DNA for regions of the genome of biological interest. The objective of our study is to assess the integrity of the clones and plates which make up the CHORI-243 library using the virtual sheep genome. Findings A series of analyses were undertaken based on the mapping the sheep BAC-end sequences (BESs to the virtual sheep genome. Overall, very few plate specific biases were identified, with only three of the 528 plates in the library significantly affected. The analysis of the number of tail-to-tail (concordant BACs on the plates identified a number of plates with lower than average numbers of such BACs. For plates 198 and 213 a partial swap of the BESs determined with one of the two primers appear to have occurred. A third plate, 341, also with a significant deficit in tail-to-tail BACs, appeared to contain a substantial number of sequences determined from contaminating eubacterial 16 S rRNA DNA. Additionally a small number of eubacterial 16 S rRNA DNA sequences were present on two other plates, 111 and 338, in the library. Conclusions The comparative genomic approach can be used to assess BAC library integrity in the absence of fingerprinting. The sequences of the sheep CHORI-243 library BACs have high integrity, especially with the corrections detailed above. The library represents a high quality resource for use by the sheep genomics community.

  13. BAC LIBRARY SCREENING OF PORCINE MILK PROTEIN GENES%利用细菌人工染色体文库(BAC)筛选猪乳蛋白基因

    Institute of Scientific and Technical Information of China (English)

    赵兴波; 铃木恒平; 李宁; 吴常信

    2001-01-01

    Gene clones of porcine Whey Acidic Protein(WAP),α s1-casein,α s2-casein,β-casein and κ-casein were selected and picked up using porcine bacteria artificial chromosome library (pBAC)and its two step-four dimensional screening system,and then discussed the one step-five dimensional screening system of pBAC library.%利用猪细菌人工染色体文库(porcine Bacteria Artificial Chromosome,pBAC)的两步-四维PCR(two step-4-D PCR)方法筛选猪乳清酸性蛋白(Whey Acidic Protein,WAP),αsl-酪蛋白,αs2-酪蛋白,β-酪蛋白,κ-酪蛋白的基因pBAC克隆,并提出了BAC基因文库一步-五维PCR(one step-5-D PCR)筛选基因的方法。

  14. Recombining overlapping BACs into a single larger BAC

    Directory of Open Access Journals (Sweden)

    Huxley Clare

    2004-01-01

    Full Text Available Abstract Background BAC clones containing entire mammalian genes including all the transcribed region and long range controlling elements are very useful for functional analysis. Sequenced BACs are available for most of the human and mouse genomes and in many cases these contain intact genes. However, large genes often span more than one BAC, and single BACs covering the entire region of interest are not available. Here we describe a system for linking two or more overlapping BACs into a single clone by homologous recombination. Results The method was used to link a 61-kb insert carrying the final 5 exons of the human CFTR gene onto a 160-kb BAC carrying the first 22 exons. Two rounds of homologous recombination were carried out in the EL350 strain of bacteria which can be induced for the Red genes. In the first round, the inserts of the two overlapping BACs were subcloned into modified BAC vectors using homologous recombination. In the second round, the BAC to be added was linearised with the very rare-cutting enzyme I-PpoI and electroporated into recombination efficient EL350 bacteria carrying the other BAC. Recombined BACs were identified by antibiotic selection and PCR screening and 10% of clones contained the correctly recombined 220-kb BAC. Conclusion The system can be used to link the inserts from any overlapping BAC or PAC clones. The original orientation of the inserts is not important and desired regions of the inserts can be selected. The size limit for the fragments recombined may be larger than the 61 kb used here and multiple BACs in a contig could be combined by alternating use of the two pBACLink vectors. This system should be of use to many investigators wishing to carry out functional analysis on large mammalian genes which are not available in single BAC clones.

  15. BacMet

    DEFF Research Database (Denmark)

    Pal, Chandan; Bengtsson-Palme, Johan; Rensing, Christopher Günther T;

    2014-01-01

    -selection. Information on metal and biocide resistance genes, including their sequences and molecular functions, is, however, scattered. Here, we introduce BacMet (http://bacmet.biomedicine.gu.se) - a manually curated database of antibacterial biocide- and metal-resistance genes based on an in-depth review...

  16. SmartOrBAC

    Directory of Open Access Journals (Sweden)

    Imane BOUIJ-PASQUIER

    2015-11-01

    Full Text Available The emergence of the Internet of Things (IoT paradigm, provides a huge scope for more streamlined living through an increase of smart services but this coincides with an increase in security and privacy concerns, therefore access control has been an important factor in the development of IoT. This work proposes an authorization access model called SmartOrBAC built around a set of security and performance requirements. This model enhances the existing OrBAC (Organization-based Access Control model and adapts it to IoT environments. SmartOrBAC separates the problem into different functional layers and then distributes processing costs between constrained devices and less constrained ones and at the same time addresses the collaborative aspect with a specific solution. This paper also presents the application of SmartOrBAC on a real example of IoT and gives a complexity study demonstrating that even though this model is extensive, it does not add additional complexity regarding traditional access control models.

  17. Library Electronic Resource Sharing Among Liberal Arts Colleges: ACS Palladian Alliance Project

    Directory of Open Access Journals (Sweden)

    Wenxian Zhang

    1997-03-01

    Full Text Available 無Effective electronic resource sharing is critical to library information services of the 1990s. Explosion of data and increased cost of information force libraries to work together, and technological advancements present the library service profession a platform for resource sharing. The Palladian Alliance Project of the Associated Colleges of the South is designed to provides ACS member institutions an effective means to enhance information access for their faculty and students, and achieve significant cost containment in the years to come.

  18. A comparative study on users’ satisfaction of library resource sharing under the circumstance of network in Nanjing

    Institute of Scientific and Technical Information of China (English)

    ZHU; Suoling; LIU; Lei

    2009-01-01

    From the perspective of information resource sharing,the authors investigated the ratio and degree of users’satisfaction with resource sharing among college libraries,public libraries and research libraries in Nanjing city.Based on the analysis and discussion about the findings,the authors summarized the library resource sharing system in satisfying users’information requirements,including its pros and cons.At last,further considerations have been provided on how to improve library resource sharing system in the region.

  19. High-resolution chromosome mapping of BACs using multi-colour FISH and pooled-BAC FISH as a backbone for sequencing tomato chromosome 6.

    Science.gov (United States)

    Szinay, Dóra; Chang, Song-Bin; Khrustaleva, Ludmila; Peters, Sander; Schijlen, Elio; Bai, Yuling; Stiekema, Willem J; van Ham, Roeland C H J; de Jong, Hans; Klein Lankhorst, René M

    2008-11-01

    Within the framework of the International Solanaceae Genome Project, the genome of tomato (Solanum lycopersicum) is currently being sequenced. We follow a 'BAC-by-BAC' approach that aims to deliver high-quality sequences of the euchromatin part of the tomato genome. BACs are selected from various libraries of the tomato genome on the basis of markers from the F2.2000 linkage map. Prior to sequencing, we validated the precise physical location of the selected BACs on the chromosomes by five-colour high-resolution fluorescent in situ hybridization (FISH) mapping. This paper describes the strategies and results of cytogenetic mapping for chromosome 6 using 75 seed BACs for FISH on pachytene complements. The cytogenetic map obtained showed discrepancies between the actual chromosomal positions of these BACs and their markers on the linkage group. These discrepancies were most notable in the pericentromere heterochromatin, thus confirming previously described suppression of cross-over recombination in that region. In a so called pooled-BAC FISH, we hybridized all seed BACs simultaneously and found a few large gaps in the euchromatin parts of the long arm that are still devoid of seed BACs and are too large for coverage by expanding BAC contigs. Combining FISH with pooled BACs and newly recruited seed BACs will thus aid in efficient targeting of novel seed BACs into these areas. Finally, we established the occurrence of repetitive DNA in heterochromatin/euchromatin borders by combining BAC FISH with hybridization of a labelled repetitive DNA fraction (Cot-100). This strategy provides an excellent means to establish the borders between euchromatin and heterochromatin in this chromosome.

  20. The Application of BAC Library in Metagenomics Research of Rumen Microbe%瘤胃元基因组BAC文库研究

    Institute of Scientific and Technical Information of China (English)

    朱雅新; 东秀珠; 黄力; 董志扬

    2007-01-01

    目前,传统的分离培养技术在瘤胃微生物的研究中仍存在一些无法逾越的障碍.元基因组文库(metagenomic libraries)及相关技术的发展,为探讨环境中未培养微生物以及复杂的微生物间关系创立了一个新的平台.将该技术应用于瘤胃微生物研究有着广阔的前景.本文综述了元基因组文库技术在瘤胃微生物研究中的应用潜力,同时介绍了初步应用BAC文库研究瘤胃微生物工作的一些进展.

  1. Annotation and BAC/PAC localization of nonredundant ESTs from drought-stressed seedlings of an indica rice

    Indian Academy of Sciences (India)

    P. Ravindra Babu; A. Chandra Sekhar; Nagabhushana Ithal; G. Markandeya; Arjula R. Reddy

    2002-04-01

    To decipher the genes associated with drought stress response and to identify novel genes in rice, we utilized 1540 high-quality expressed sequence tags (ESTs) for functional annotation and mapping to rice genomic sequences. These ESTs were generated earlier by 3′-end single-pass sequencing of 2000 cDNA clones from normalized cDNA libraries constructed from drought-stressed seedlings of an indica rice. A rice UniGene set of 1025 transcripts was constructed from this collection through the BLASTN algorithm. Putative functions of 559 nonredundant ESTs were identified by BLAST similarity search against public databases. Putative functions were assigned at a stringency E value of 10-6 in BLASTN and BLASTX algorithms. To understand the gene structure and function further, we have utilized the publicly available finished and unfinished rice BAC/PAC (BAC, bacterial artificial chromosome; PAC, P1 artificial chromosome) sequences for similarity search using the BLASTN algorithm. Further, 603 nonredundant ESTs have been mapped to BAC/PAC clones. BAC clones were assigned by a homology of above 95% identity along 90% of EST sequence length in the aligned region. In all, 700 ESTs showed rice EST hits in GenBank. Of the 325 novel ESTs, 128 were localized to BAC clones. In addition, 127 ESTs with identified putative functions but with no homology in IRGSP (International Rice Genome Sequencing Program) BAC/PAC sequences were mapped to the Chinese WGS (whole genome shotgun contigs) draft sequence of the rice genome. Functional annotation uncovered about a hundred candidate ESTs associated with abiotic stress in rice and Arabidopsis that were previously reported based on microarray analysis and other studies. This study is a major effort in identifying genes associated with drought stress response and will serve as a resource to rice geneticists and molecular biologists.

  2. The Study of Analytical Model of Library Electronic Resources Usage-A Case of Medical Electronic Resources

    Directory of Open Access Journals (Sweden)

    Chung-Yen Yu

    2014-10-01

    Full Text Available With the advents of internet, the importance of electronic resources is growing. Due to the increasing expensiveness of electronic resources, university libraries normally received budgets from parent institutions annually. They necessarily applied effective and systematic methods for decision making in electronic resources purchase or re-subscription. However, there are some difficulties in practices: First of all, libraries are unable to receive user records; second, the COUNTER statistics does not include details about users and their affiliation. As a result, one cannot conduct advanced user analysis based on the usage of users, institutions, and departments. To overcome the difficulties, this study presents a feasible model to analyze electronic resource usage effectively and flexibly. We set up a proxy server to collect actual usage raw data. By analyzing items in internet browsing records, associated with original library automatic system, this study aims at exploring how to use effective ways to analyze big data of website log data. We also propose the process of how original data to be transformed, cleared, integrated, and demonstrated. This study adopted a medical university library and its subscription of medical electronic resources as a case. Our data analysis includes (1 year of subscription,(2 title of journal, (3 affiliation, (4 subjects, and (5 specific journal requirements, etc. The findings of the study are contributed to obtain further understanding in policy making and user behavior analysis. The integrated data provides multiple applications in informatics research, information behavior, bibliomining, presenting diverse views and extended issues for further discussion.

  3. Managing information resources in libraries collection management in theory and practice

    CERN Document Server

    Clayton, Peter

    2006-01-01

    The management of information resources in libraries is of greater importance in the digital world. This book encompasses different areas of collection management and cover topics, such as: collection management in the organizational context; collection development policies; selection principles and resources; budget management; and more.

  4. Leveraging Standards To Enhance the Library's Goals and Resources.

    Science.gov (United States)

    Marriott, Catherine

    2002-01-01

    Discussion of the use of technology in primary grades focuses on a pilot project in the Orchard Park (New York) elementary schools that used computers to teach a nonfiction unit for English language arts, focusing on the New York State learning standards. Describes the role of school library media specialists and benefits to students. (LRW)

  5. Library Resources in Special Areas of Music: Film Music.

    Science.gov (United States)

    Wright, H. Stephen

    Intended as an orientation for music librarians unfamiliar with the film music field, this presentation addresses the most common film music questions received from library patrons, including queries about composers, soundtrack albums, the subject of the music, and scores, and describes the basic film music reference sources to consult for…

  6. Factors Influencing Virtual Patron satisfaction with Online Library Resources and Services

    Directory of Open Access Journals (Sweden)

    Katherine Tyler

    2011-07-01

    Full Text Available College students are accessing virtual libraries whether they are on campus or learning from a distance. Academic institutions serving virtual patrons must remain focused on meeting the needs of those library users by continually examining their preferences, their searching behavior, and the information they seek. The purpose of this research was to determine if virtual patrons are satisfied with the resources and services being provided by a university’s online library. Following a web-based survey, demographic characteristics of students were analyzed to determine if any influenced students’ satisfaction. Using analysis of variance, correlation, and descriptive statistics, several demographic factors were found to influence student satisfaction with the library’s online resources: age, gender, achieved educational level, student status, and computer experience. One factor, computer experience, was found to influence student satisfaction with the library’s online services.Overall, students reported satisfaction with the university’s online library resources and services. Comments submitted to open-ended questions regarding areas for improvement to the online library provide library administrators with avenues for development to increase awareness of library services, focus improvement in navigation, and enhance student satisfaction.

  7. HIV/AIDS Information Resources from the National Library of Medicine-STOP

    Energy Technology Data Exchange (ETDEWEB)

    Templin-Branner, W. and N. Dancy

    2010-06-15

    The HIV/AIDS Information Resources from the National Library of Medicine training is designed specifically for the UNCFSP HBCU Screening, Testing, Outreach, and Prevention (STOP) HIV/AIDS Program project members to provide valuable health information resources from the National Library of Medicine and other reliable sources to increase awareness of the wealth of treatment information and educational materials that are available on the Internet and to improve prevention and treatment education for their clients. These resources will also meet the needs of community-based organizations

  8. Localization and Characterization of 170 BAC-derived clones and mapping of Ninety-Four Microsatellites in the Hessian Fly

    Science.gov (United States)

    Ninety-four microsatellites from enriched genomic libraries of Hessian fly (Mayetiola destructor (Say)) were localized to 170 cognate clones in a Hessian fly bacterial artificial chromosome (BAC) library. These microsatellite-positive BAC clones were physically mapped to polytene chromosomes by fl...

  9. BAC end sequencing of Pacific white shrimp Litopenaeus vannamei: a glimpse into the genome of Penaeid shrimp

    Science.gov (United States)

    Zhao, Cui; Zhang, Xiaojun; Liu, Chengzhang; Huan, Pin; Li, Fuhua; Xiang, Jianhai; Huang, Chao

    2012-05-01

    Little is known about the genome of Pacific white shrimp ( Litopenaeus vannamei). To address this, we conducted BAC (bacterial artificial chromosome) end sequencing of L. vannamei. We selected and sequenced 7 812 BAC clones from the BAC library LvHE from the two ends of the inserts by Sanger sequencing. After trimming and quality filtering, 11 279 BAC end sequences (BESs) including 4 609 pairedends BESs were obtained. The total length of the BESs was 4 340 753 bp, representing 0.18% of the L. vannamei haploid genome. The lengths of the BESs ranged from 100 bp to 660 bp with an average length of 385 bp. Analysis of the BESs indicated that the L. vannamei genome is AT-rich and that the primary repeats patterns were simple sequence repeats (SSRs) and low complexity sequences. Dinucleotide and hexanucleotide repeats were the most common SSR types in the BESs. The most abundant transposable element was gypsy, which may contribute to the generation of the large genome size of L. vannamei. We successfully annotated 4 519 BESs by BLAST searching, including genes involved in immunity and sex determination. Our results provide an important resource for functional gene studies, map construction and integration, and complete genome assembly for this species.

  10. BAC end sequencing of Pacific white shrimp Litopenaeus vannamei: a glimpse into the genome of Penaeid shrimp

    Institute of Scientific and Technical Information of China (English)

    ZHAO Cui; ZHANG Xiaojun; LIU Chengzhang; HUAN Pin; LI Fuhua; XIANG Jianhai; HUANG Chao

    2012-01-01

    Little is known about the genome of Pacific white shrimp (Litopenaeus vannamei).To address this,we conducted BAC (bacterial artificial chromosome) end sequencing of L.vannamei.We selected and sequenced 7 812 BAC clones from the BAC library LvHE from the two ends of the inserts by Sanger sequencing.After trimming and quality filtering,11 279 BAC end sequences (BESs) including 4 609 pairedends BESs were obtained.The total length of the BESs was 4 340 753 bp,representing 0.18% of the L.vannamei haploid genome.The lengths of the BESs ranged from 100 bp to 660 bp with an average length of 385 bp.Analysis of the BESs indicated that the L.vannamei genome is AT-rich and that the primary repeats patterns were simple sequence repeats (SSRs) and low complexity sequences.Dinucleotide and hexanucleotide repeats were the most common SSR types in the BESs.The most abtmdant transposable element was gypsy,which may contribute to the generation of the large genome size of L.vannamei.We successfully annotated 4 519 BESs by BLAST searching,including genes involved in immunity and sex determination.Our results provide an important resource for functional gene studies,map construction and integration,and complete genome assembly for this species.

  11. Publishing, Books and Library Resources: Brazil and Argentina.

    Science.gov (United States)

    National Book Committee, Inc., New York, NY.

    This survey synthesizes and analyzes the book resources and book requirements of Brazil, with particular reference to the educational scene, in terms of local production and distribution resources and capabilities. Information on the geography, political establishment, economy, and educational system of Brazil is presented in the introductory…

  12. The Open Library at AU (Athabasca University: Supporting Open Access and Open Educational Resources

    Directory of Open Access Journals (Sweden)

    Colin Elliott

    2015-04-01

    Full Text Available To address challenges that learners, course creators, librarians and academics involved with OER and MOOCs are facing when looking for scholarly materials, Athabasca University Library has initiated the development of the Open Library at AU. This open library is a full library website that provides easy access to open and free resources. Tools and information literacy tutorials are also included to enable learners, researchers, and others to find, evaluate, and use the information they need for their open learning course or research. Many of the challenges that those involved in open learning face are addressed by the open library and the potential impact it can have on open learning and knowledge sharing is tremendous. http://dx.doi.org/10.5944/openpraxis.7.2.196

  13. Identification of three wheat globulin genes by screening a Triticum aestivum BAC genomic library with cDNA from a diabetes-associated globulin

    Directory of Open Access Journals (Sweden)

    MacFarlane Amanda J

    2009-07-01

    Full Text Available Abstract Background Exposure to dietary wheat proteins in genetically susceptible individuals has been associated with increased risk for the development of Type 1 diabetes (T1D. Recently, a wheat protein encoded by cDNA WP5212 has been shown to be antigenic in mice, rats and humans with autoimmune T1D. To investigate the genomic origin of the identified wheat protein cDNA, a hexaploid wheat genomic library from Glenlea cultivar was screened. Results Three unique wheat globulin genes, Glo-3A, Glo3-B and Glo-3C, were identified. We describe the genomic structure of these genes and their expression pattern in wheat seeds. The Glo-3A gene shared 99% identity with the cDNA of WP5212 at the nucleotide and deduced amino acid level, indicating that we have identified the gene(s encoding wheat protein WP5212. Southern analysis revealed the presence of multiple copies of Glo-3-like sequences in all wheat samples, including hexaploid, tetraploid and diploid species wheat seed. Aleurone and embryo tissue specificity of WP5212 gene expression, suggested by promoter region analysis, which demonstrated an absence of endosperm specific cis elements, was confirmed by immunofluorescence microscopy using anti-WP5212 antibodies. Conclusion Taken together, the results indicate that a diverse group of globulins exists in wheat, some of which could be associated with the pathogenesis of T1D in some susceptible individuals. These data expand our knowledge of specific wheat globulins and will enable further elucidation of their role in wheat biology and human health.

  14. International Students' Perception of Library Services and Information Resources in Chinese Academic Libraries

    Science.gov (United States)

    Nzivo, Charles N.; Chuanfu, Chen

    2013-01-01

    With the growth of international student enrollment in Chinese universities, user perception has become an area of developing research in academic libraries. China's policy of opening up has led to the education and economic reforms allowing major universities to enroll international students. These changes have created an increasing need for…

  15. Generation of a BAC-based physical map of the melon genome

    Directory of Open Access Journals (Sweden)

    Puigdomènech Pere

    2010-05-01

    Full Text Available Abstract Background Cucumis melo (melon belongs to the Cucurbitaceae family, whose economic importance among horticulture crops is second only to Solanaceae. Melon has high intra-specific genetic variation, morphologic diversity and a small genome size (450 Mb, which make this species suitable for a great variety of molecular and genetic studies that can lead to the development of tools for breeding varieties of the species. A number of genetic and genomic resources have already been developed, such as several genetic maps and BAC genomic libraries. These tools are essential for the construction of a physical map, a valuable resource for map-based cloning, comparative genomics and assembly of whole genome sequencing data. However, no physical map of any Cucurbitaceae has yet been developed. A project has recently been started to sequence the complete melon genome following a whole-genome shotgun strategy, which makes use of massive sequencing data. A BAC-based melon physical map will be a useful tool to help assemble and refine the draft genome data that is being produced. Results A melon physical map was constructed using a 5.7 × BAC library and a genetic map previously developed in our laboratories. High-information-content fingerprinting (HICF was carried out on 23,040 BAC clones, digesting with five restriction enzymes and SNaPshot labeling, followed by contig assembly with FPC software. The physical map has 1,355 contigs and 441 singletons, with an estimated physical length of 407 Mb (0.9 × coverage of the genome and the longest contig being 3.2 Mb. The anchoring of 845 BAC clones to 178 genetic markers (100 RFLPs, 76 SNPs and 2 SSRs also allowed the genetic positioning of 183 physical map contigs/singletons, representing 55 Mb (12% of the melon genome, to individual chromosomal loci. The melon FPC database is available for download at http://melonomics.upv.es/static/files/public/physical_map/. Conclusions Here we report the construction

  16. The Physic Source, a ComPADRE digital library, focusing on resources for teaching introductory physics

    Science.gov (United States)

    Mzoughi, Taha

    2012-03-01

    The Physics Source (http://www.physicssource.org/), a component of ComPADRE, was designed to help faculty teaching introductory physics courses identify and use high quality relevant curriculum and pedagogical resources in their teaching. Faculty can use to Physics Source to find, share, and collaborate on building high-quality, effective teaching and learning materials. Users can also organize the content they like, and submit their own resources for inclusion in the library. Among the content included in the library are simulations, lab activities, classroom demonstrations, videos, information on some teaching techniques, and physics education research articles.

  17. Linking Formal and Informal Science Education: A Successful Model using Libraries, Volunteers and NASA Resources

    Science.gov (United States)

    Race, M. S.; Lafayette Library; Learning Center Foundation (Lllcf)

    2011-12-01

    In these times of budget cuts, tight school schedules, and limited opportunities for student field trips and teacher professional development, it is especially difficult to expose elementary and middle school students to the latest STEM information-particularly in the space sciences. Using our library as a facilitator and catalyst, we built a volunteer-based, multi-faceted, curriculum-linked program for students and teachers in local middle schools (Grade 8) and showcased new astronomical and planetary science information using mainly NASA resources and volunteer effort. The project began with the idea of bringing free NASA photo exhibits (FETTU) to the Lafayette and Antioch Libraries for public display. Subsequently, the effort expanded by adding layers of activities that brought space and science information to teachers, students and the pubic at 5 libraries and schools in the 2 cities, one of which serves a diverse, underserved community. Overall, the effort (supported by a pilot grant from the Bechtel Foundation) included school and library based teacher workshops with resource materials; travelling space museum visits with hands-on activities (Chabot-to-Go); separate powerpoint presentations for students and adults at the library; and concurrent ancillary space-related themes for young children's programs at the library. This pilot project, based largely on the use of free government resources and online materials, demonstrated that volunteer-based, standards-linked STEM efforts can enhance curriculum at the middle school, with libraries serving a special role. Using this model, we subsequently also obtained a small NASA-Space Grant award to bring star parties and hand-on science activities to three libraries this Fall, linking with numerous Grade 5 teachers and students in two additional underserved areas of our county. It's not necessary to reinvent the wheel, you just collect the pieces and build on what you already have.

  18. BAC-end sequence-based SNP mining in Allotetraploid Cotton (Gossypium) utilizing re-sequencing data, phylogenetic inferences and perspectives for genetic mapping

    Science.gov (United States)

    A bacterial artificial chromosome (BAC) library and BAC-end sequences for Gossypium hirsutum L. have recently been developed. Here we report on genomic-based genome-wide SNP mining utilizing re-sequencing data with a BAC-end sequence reference for twelve G. hirsutum L. lines, one G. barbadense L. li...

  19. Sequencing of 6.7 Mb of the melon genome using a BAC pooling strategy

    Directory of Open Access Journals (Sweden)

    Garcia-Mas Jordi

    2010-11-01

    Full Text Available Abstract Background Cucumis melo (melon belongs to the Cucurbitaceae family, whose economic importance among horticulture crops is second only to Solanaceae. Melon has a high intra-specific genetic variation, morphologic diversity and a small genome size (454 Mb, which make it suitable for a great variety of molecular and genetic studies. A number of genetic and genomic resources have already been developed, such as several genetic maps, BAC genomic libraries, a BAC-based physical map and EST collections. Sequence information would be invaluable to complete the picture of the melon genomic landscape, furthering our understanding of this species' evolution from its relatives and providing an important genetic tool. However, to this day there is little sequence data available, only a few melon genes and genomic regions are deposited in public databases. The development of massively parallel sequencing methods allows envisaging new strategies to obtain long fragments of genomic sequence at higher speed and lower cost than previous Sanger-based methods. Results In order to gain insight into the structure of a significant portion of the melon genome we set out to perform massive sequencing of pools of BAC clones. For this, a set of 57 BAC clones from a double haploid line was sequenced in two pools with the 454 system using both shotgun and paired-end approaches. The final assembly consists of an estimated 95% of the actual size of the melon BAC clones, with most likely complete sequences for 50 of the BACs, and a total sequence coverage of 39x. The accuracy of the assembly was assessed by comparing the previously available Sanger sequence of one of the BACs against its 454 sequence, and the polymorphisms found involved only 1.7 differences every 10,000 bp that were localized in 15 homopolymeric regions and two dinucleotide tandem repeats. Overall, the study provides approximately 6.7 Mb or 1.5% of the melon genome. The analysis of this new data has

  20. Construction and Characterization of a Bacterial Artificial Chromosome Library for the A-Genome of Cotton (G. arboreum L.

    Directory of Open Access Journals (Sweden)

    Yan Hu

    2011-01-01

    Full Text Available A bacterial artificial chromosome (BAC library for the A-genome of cotton has been constructed from the leaves of G. arboreum L cv. Jianglinzhongmian. It is used as elite A-genome germplasm resources in the present cotton breeding program and has been used to build a genetic reference map of cotton. The BAC library consists of 123,648 clones stored in 322 384-well plates. Statistical analysis of a set of 103 randomly selected BAC clones indicated that each clone has an average insert length of 100.2 kb per plasmid, with a range of 30 to 190 kb. Theoretically, this represents 7.2 haploid genome equivalents based on an A-genome size of 1697 Mb. The BAC library has been arranged in column pools and superpools allowing screening with various PCR-based markers. In the future, the A-genome cotton BAC library will serve as both a giant gene resource and a valuable tool for map-based gene isolation, physical mapping and comparative genome analysis.

  1. Up a Creek? Here's a Paddle: An Annotated Bibliography of Recent Resources for New or Occasional Library Instructors.

    Science.gov (United States)

    Ashmore, Beth

    2002-01-01

    Provides resources for library instructors who are teaching with minimal formal training in the field of education. Reports that the professional library literature on this topic is plentiful and can provide both theoretical and practical help for library instructors in the areas of learning theory, technology use, and teaching/presentation style.…

  2. Effective Techniques for the Promotion of Library Services and Resources

    Science.gov (United States)

    Yi, Zhixian

    2016-01-01

    Introduction: This study examines how Australian academic librarians perceive techniques for promoting services and resources, and the factors affecting the perceptions regarding effectiveness of techniques used. Method: Data were collected from an online survey that was sent to 400 academic librarians in thirty-seven Australian universities. The…

  3. Construction and characterization of a Schistosoma mansoni bacterial artificial chromosome library.

    Science.gov (United States)

    Le Paslier, M C; Pierce, R J; Merlin, F; Hirai, H; Wu, W; Williams, D L; Johnston, D; LoVerde, P T; Le Paslier, D

    2000-04-15

    A bacterial artificial chromosome (BAC) library has been established from genomic DNA isolated from the trematode parasite of human, Schistosoma mansoni. This library consists of more than 21,000 recombinant clones carrying inserts in the pBeloBAC11 vector. The mean insert size was 100 kb, representing an approximate 7.95-fold genome coverage. Library screening with eight chromosome-specific or single-copy gene probes yielded between 1 and 9 positive clones, and none of those tested was absent from the library. End sequences were obtained for 93 randomly selected clones, and 37 showed sequence identity to S. mansoni sequences (ESTs, genes, or repetitive sequences). A preliminary analysis by fluorescence in situ hybridization localized 8 clones on schistosome chromosomes 1 (2 clones), 2, 3, 5, Z, and W (3 clones). This library provides a new resource for the physical mapping and sequencing of the genome of this important human pathogen.

  4. Integration of multimedia content and e-learning resources in a digital library

    Science.gov (United States)

    Pascual, Mireia; Ferran, Núria; Minguillón, Julià

    2006-01-01

    In this paper we describe a proposal for multimedia and e-learning content description based on standards interoperability within a digital library environment integrated in a virtual campus. In any virtual e-learning environment, a complex scenario which usually includes a digital library or, at least, a repository of learning resources, different levels of description are needed for all the elements: learning resources, multimedia content, activities, roles, etc. These elements can be described using library, e-learning and multimedia standards, depending on the specific needs of each particular scenario of use, but this might lead to an undesirable duplication of metadata, and to inefficient content queries and maintenance. Furthermore, there is a lack of semantic descriptions which makes all these contents merely become digital objects in the digital library, without exploiting all the possibilities in a e-learning virtual environment. Due to its flexibility and completeness, we propose to use the MPEG-7 standard for describing all the learning resources in the digital library, combined with the use of an ontology for a formal description of the learning process. The equivalences of Dublin Core, LOM and MPEG-7 standards are outlined, and the requirements of a proposal for a MPEG-7 based representation for all the contents in the digital library and the virtual classroom are described. The intellectual property policies for content sharing both within and among organizations are also addressed. With such proposal, it would be possible to build complex multimedia courses from a repository of learning objects using the digital library as the core repository.

  5. Council on Library and Information Resources: Annual Report, 2007-2008

    Science.gov (United States)

    Council on Library and Information Resources, 2008

    2008-01-01

    This annual report of the Council on Library and Information Resources (CLIR) contains an overview of activities of the Council between July 1, 2007 and June 30, 2008. These include programs, advisory groups, grants and contracts, and financial statements. It also includes a list of staff, a letter from the chairperson Paula Kaufman, and a message…

  6. Council on Library and Information Resources: Annual Report, 2006-2007

    Science.gov (United States)

    Council on Library and Information Resources, 2007

    2007-01-01

    This annual report of the Council on Library and Information Resources (CLIR) contains an overview of activities of the Council between July 1, 2006 and June 30, 2007. These include programs, awards, publications, advisory groups, grants and contracts, and financial statements. It also includes a list of staff, a letter from the chairperson Paula…

  7. QR Codes as Finding Aides: Linking Electronic and Print Library Resources

    Science.gov (United States)

    Kane, Danielle; Schneidewind, Jeff

    2011-01-01

    As part of a focused, methodical, and evaluative approach to emerging technologies, QR codes are one of many new technologies being used by the UC Irvine Libraries. QR codes provide simple connections between print and virtual resources. In summer 2010, a small task force began to investigate how QR codes could be used to provide information and…

  8. Remote Electronic Resources and the OPAC: Illustrated by the Unisa Library Experience.

    Science.gov (United States)

    Van der Merwe, Ina; Van Eeden, Welna; Hartzer, Sandra

    This paper describes the Unisa (University of South Africa) Library's experience with cataloging remote electronic resources, including electronic journals, electronic text files, online databases, digital images, Unisa campus Web sites, and mailing list discussions. The first section discusses the decision to add bibliographic references for…

  9. Perceptions of Libraries and Information Resources: A Report to the OCLC Membership

    Science.gov (United States)

    OCLC Online Computer Library Center, Inc., 2005

    2005-01-01

    "Perceptions of Libraries and Information Resources" (2005) summarizes findings of an international study on information-seeking habits and preferences. With extensive input from hundreds of librarians and OCLC staff, the OCLC Market Research team developed a project and commissioned Harris Interactive Inc. to survey a representative…

  10. Council on Library Resources, Inc. Twenty-Sixth Annual Report/1982.

    Science.gov (United States)

    Council on Library Resources, Inc., Washington, DC.

    This report describes the activities of the Council on Library Resources, Inc. (CLR) during the 1981-82 fiscal year. Contents include lists of members of the Council, the Council Board of Directors, and the Council staff; a listing of CLR committee members and officers; a preface; a brief statement of highlights of the year's activities and future…

  11. Council on Library Resources, Inc. Twenty-Fifth Annual Report, 1981.

    Science.gov (United States)

    Council on Library Resources, Inc., Washington, DC.

    This report describes the activities of the Council on Library Resources, Inc. (CLR) during the 1980-1981 fiscal year. Contents include lists of members of the Council, the Council Board of Directors, and the Council staff; a preface; a brief statement of highlights of the year's activities; and sections devoted to activities in specific program…

  12. Collection Development Policy. Media Retrieval Services--Pierce Library. Learning Resources. 1981 Rev.

    Science.gov (United States)

    Sawin, Philip, Jr., Ed.

    Policies for the selection, acquisition, and weeding of library materials, based on collection needs stated by academic departments and staff inputs, are listed for a university learning resource center. An alphabetical index is provided of major subject areas and subdivisions, which are assigned one of four level designators indicating the depth…

  13. A preliminary analysis of library holdings as compared to the basic resources for pharmacy education list.

    Science.gov (United States)

    Vaughan, K T L V; Lerner, Rachel C

    2013-01-01

    The catalogs of 11 university libraries were analyzed against the Basic Resources for Pharmaceutical Education (BRPE) to measure the percent coverage of the core total list as well as the core sublist. There is no clear trend in this data to link school age, size, or rank with percentage of coverage of the total list or the "First Purchase" core list when treated as independent variables. Approximately half of the schools have significantly higher percentages of core titles than statistically expected. Based on this data, it is difficult to predict what percentage of titles on the BRPE a library will contain.

  14. Analytical Study of Usage of Electronic Information Resources at Pharmacopoeial Libraries in India

    Directory of Open Access Journals (Sweden)

    Sunil Tyagi

    2014-02-01

    Full Text Available The objective of this study is to know the rate and purpose of the use of e-resource by the scientists at pharmacopoeial libraries in India. Among other things, this study examined the preferences of the scientists toward printed books and journals, electronic information resources, and pattern of using e-resources. Non-probability sampling specially accidental and purposive technique was applied in the collection of primary data through administration of user questionnaire. The sample respondents chosen for the study consists of principle scientific officer, senior scientific officer, scientific officer, and scientific assistant of different division of the laboratories, namely, research and development, pharmaceutical chemistry, pharmacovigilance, pharmacology, pharmacogonosy, and microbiology. The findings of the study reveal the personal experiences and perceptions they have had on practice and research activity using e-resource. The major findings indicate that of the total anticipated participants, 78% indicated that they perceived the ability to use computer for electronic information resources. The data analysis shows that all the scientists belonging to the pharmacopoeial libraries used electronic information resources to address issues relating to drug indexes and compendia, monographs, drugs obtained through online databases, e-journals, and the Internet sources—especially polices by regulatory agencies, contacts, drug promotional literature, and standards.

  15. Library and Information Resources and Users of Digital Resources in the Humanities

    Science.gov (United States)

    Warwick, Claire; Terras, Melissa; Galina, Isabel; Huntington, Paul; Pappa, Nikoleta

    2008-01-01

    Purpose: The purpose of this article is to discuss the results of the Log Analysis of Internet Resources in the Arts and Humanities (LAIRAH) study. It aims to concentrate upon the use and importance of information resources, physical research centres and digital finding aids in scholarly research. Design/methodology/approach: Results are presented…

  16. Caribbean Equal Access Program: HIV/AIDS Information Resources from the National Library of Medicine

    Energy Technology Data Exchange (ETDEWEB)

    Nancy Dancy, NLM, and Wilma Templin-Branner, ORISE

    2009-01-01

    As the treatment and management of HIV/AIDS continues to evolve with new scientific breakthroughs, treatment discoveries, and management challenges, it is difficult for people living with HIV/AIDS and those who care for them to keep up with the latest information on HIV/AIDS prevention, treatment, and research. The National Library of Medicine, of the National Institutes of Health, has a wealth of health information resources freely available on the Internet to address these needs.

  17. Resource Sharing Among ISRO Libraries : a Case Study of Consortia Approach

    OpenAIRE

    M.S. Sridhar

    2002-01-01

    The paper presents a practical step by step approach and efforts made to achieve huge savings in acquisition expenditures and creative resources sharing among a group of libraries working under a single parent institution, namely, Indian Space Research Organisation (ISRO). Actual cases of substantial savings in subscription to costly journals, CD-ROM databases and conference volumes in the way of consortia price and/or combination (print and electronic) subscription prices are depicted with...

  18. Using SemanticScuttle for managing lists of recommended resources on a library website

    Directory of Open Access Journals (Sweden)

    Tomasz Neugebauer

    2015-01-01

    Full Text Available Concordia University Libraries has adopted SemanticScuttle, an open source and locally-hosted PHP/MySQL application for social bookmarking, as an alternative to Delicious for managing lists of recommended resources on the library’s website. Two implementations for displaying feed content from SemanticScuttle were developed: (1 using the Google Feed API and (2 using direct SQL access to SemanticScuttle’s database.

  19. Improving the comparative map of SSC2p-q13 by sample sequencing of BAC clones

    NARCIS (Netherlands)

    Rattink, A.P.; Jungerius, B.J.; Faivre, M.; Chardon, P.; Harlizius, B.; Groenen, M.A.M.

    2001-01-01

    To improve the comparative map for pig chromosome 2 and increase the gene density on this chromosome, a porcine bacterial artificial chromosome (BAC) library was screened with 17 microsatellite markers and 18 genes previously assigned to pig chromosome 2. Fifty-one BAC clones located in the region o

  20. Research for Design: Exploring Student and Instructor Attitudes toward Accessing Library Resources and Services from Course Management Systems (CMS

    Directory of Open Access Journals (Sweden)

    Merinda McLure

    2010-10-01

    Full Text Available The authors report a study concerning student and instructor attitudes toward accessing library resources and services from within course management systems (CMS. In spring 2008, the authors conducted semi-structured interviews with a small population of students and instructors at the University of California, Berkeley (UCB and at Colorado State University (CSU. They asked participants to respond to examples of library integrations in course management systems at other institutions and to report their local experiences with both the campus CMS and library services. Participant responses frequently challenged and complicated the authors’ assumptions about best practices in integrating a library presence and library services in CMS. The interview findings are discussed thematically, in relation to higher education and library literature, and can inform librarians’ efforts to integrate an effective library presence in a campus course management system. This study might be readily adapted for implementation at other institutions.

  1. Building a Digital Library from the Ground Up: an Examination of Emergent Information Resources in the Machine Learning Community

    Science.gov (United States)

    Cunningham, Sally Jo

    The current crop of digital libraries for the computing community are strongly grounded in the conventional library paradigm: they provide indexes to support searching of collections of research papers. As such, these digital libraries are relatively impoverished; the present computing digital libraries omit many of the documents and resources that are currently available to computing researchers, and offer few browsing structures. These computing digital libraries were built 'top down': the resources and collection contents are forced to fit an existing digital library architecture. A 'bottom up' approach to digital library development would begin with an investigation of a community's information needs and available documents, and then design a library to organize those documents in such a way as to fulfill the community's needs. The 'home grown', informal information resources developed by and for the machine learning community are examined as a case study, to determine the types of information and document organizations 'native' to this group of researchers. The insights gained in this type of case study can be used to inform construction of a digital library tailored to this community.

  2. Out on the Web: The Relationship between Campus Climate and GLBT-Related Web-based Resources in Academic Libraries

    Science.gov (United States)

    Ciszek, Matthew P.

    2011-01-01

    This article explores the relationship between the perceived campus environment for gay, lesbian, bisexual, and transgender (GLBT) students at colleges and universities and how academic libraries have deployed GLBT-related resources on the Web. Recommendations are made for increasing GLBT-related materials and information in academic libraries.…

  3. A Study of Assessment of Library E-resource Metasearch System

    Directory of Open Access Journals (Sweden)

    Fan-Chuan Tseng

    2008-12-01

    Full Text Available As the emergent development of computer and network technology, digital library and information service has become an important approach for users to increase their academic as well as job performance due to the authorized, accurate, and reliable information. In the realm of information management, the exploration of factors influencing information system utilization and the evaluation of benefits are two critical issues. However, related studies focus more on the system functions and usually ignore the effects of individual cognition and social influence. In this study, we extend the IS success model with two additional factors-self-efficacy and subjective norm, trying to explore the potential influences on digital library usage, user satisfaction and performance.This research takes the Chang Jung Christian University library E-resources Metasearch system as the case study. A questionnaire was developed and finally got 160 useful student samples to test and verify the hypotheses. The research results confirmed that self-efficacy and subjective norm can significantly affect the usage of E-resource Metasearch System. Subsequently, the users feel satisfied with the system, and increase their academic performance. Finally, we provide discussion, implications and suggestions in two aspects – cooperating more with educational programs to increase users’ information literacy, and improving the customer-oriented system interface, functions, and services to enhance its quality. [Article content in Chinese

  4. A general method to modify BACs to generate large recombinant DNA fragments.

    Science.gov (United States)

    Shen, Wei; Huang, Yue; Tang, Yi; Liu, De-Pei; Liang, Chih-Chuan

    2005-11-01

    Bacterial artificial chromosome (BAC) has the capacity to clone DNA fragments in excess of 300 kb. It also has the considerable advantages of stable propagation and ease of purification. These features make BAC suitable in genetic research, such as library construction, transgenic mice production, and gene targeting constructs. Homologous recombination in Escherichia coli, a process named recombineering, has made the modification of BACs easy and reliable. We report here a modified recombineering method that can efficiently mediate the fusion of large DNA fragments from two or more different BACs. With the introduction of kanamycin-resistant gene and proposed rare-cutting restriction endonuclease (RCRE) sites into two BACs, a 82.6-kb DNA fragment containing the inverted human alpha-globin genes (theta, alpha1, alpha2, and zeta) from BAC191K2 and the locus control region (LCR) of human beta-globin gene locus (from the BAC186D7) was reconstructed. This approach for combining different BAC DNA fragments should facilitate many kinds of genomic experiments.

  5. Integration of Audio Resources into a Digital Library: The BEIC Case Study

    Directory of Open Access Journals (Sweden)

    Adriano Baratè

    2015-06-01

    Full Text Available The focus of this paper is on the integration of audio resources with other content types in digital libraries. As a case study, we will present the most recent initiative of the Biblioteca Europea di Informazione e Cultura (BEIC, an Italian institution that pursues educational and instructional goals through the realization and management of a multimedia, free access, open shelf library. A new audio section will be added to the already-existing digital archive, allowing users to listen to about 1000 classical recordings in a multi-platform and cross-browser manner. This experience involves a number of heterogeneous fields, ranging from musicology to computer programming, from cataloging to digitization and archiving. In this paper, we will apply a bottom-up technique in order to provide a generalization of the specific case study, thus suggesting a methodological approach for similar initiatives.

  6. The eTOX Library of Public Resources for in Silico Toxicity Prediction.

    Science.gov (United States)

    Cases, M; Pastor, M; Sanz, F

    2013-01-01

    (1000-1500 characters) In spite of the increasing amount of public access resources that offer original data related to drug toxicology, the successful exploitation of such data for the development of in silico predictive models is still limited by the quality of the data available, its integrability and its coverage for each toxicity endpoint. This work describes the strategy developed by the IMI eTOX consortium for identifying and compiling data and other related resources from the biomedical literature and a wide spectrum of public on-line sources. The main result of this effort is a large web-based structured library containing links to articles of toxicological relevance (data that can be used for modeling purposes, computational models, and toxicity mechanisms), public databases, standardized vocabularies and modeling tools. All this material has been manually reviewed, systematically evaluated and grouped into different categories. The library has been made public at the eTOX website (http://www.etoxproject.eu/), where it is updated on a monthly basis, constituting a useful resource for affording the in silico toxicity prediction of novel drug candidates.

  7. Transition from in library use of resources to outside library use: the impact of the Internet on information seeking behavior of medical students and faculty.

    Science.gov (United States)

    Tao, Donghua; Demiris, George; Graves, Rebecca S; Sievert, MaryEllen

    2003-01-01

    Advances in information technology have introduced both new capabilities and interesting challenges in accessing medical literature. More and more information resources exist in electronic format, such as online databases, journals, books, etc. instead of the traditional print format. In late 1998, there were thirty-five journal titles available online; in 2001, the number rose to over 4,000.1 Desk-top access to online resources is changing library use patterns, which challenges libraries to adjust to this transformed information access environment. Studies of the impact of the internet on information seeking behavior of users in medical environments could provide very valuable information for medical libraries seeking to adapt to this rapid and great evolution. This study aims to explore the impact of the Internet on information seeking behavior of medical students and faculty and their medical library use, to address the possible reasons for this change of information seeking behavior, and to identify the measures essential to the transition from traditional in-library use of resources to remote access. This study is conducted in two phases.

  8. Teachers' Link to Electronic Resources in the Library Media Center: A Local Study of Awareness, Knowledge, and Influence

    Science.gov (United States)

    Williams, Teresa D.; Grimble, Bonnie J.; Irwin, Marilyn

    2004-01-01

    High school students often use online databases and the Internet in the school library media center (SLMC) to complete teachers' assignments. This case study used a survey to assess teachers' awareness of electronic resources, and to determine whether their directions influence student use of these resources in the SLMC. Participants were teachers…

  9. Providing Curriculum Support in the School Library Media Center: Resource Alignment, or How To Eat an Elephant.

    Science.gov (United States)

    Lowe, Karen

    2003-01-01

    Discusses the process of weeding, updating, and building a school library media collection that supports the state curriculum. Explains resource alignment, a process for using the shelf list as a tool to analyze and align media center resources to state curricula, and describes a five-year plan and its usefulness for additional funding. (LRW)

  10. Construction of A Deep Coverage Rice BAC Library and Identification of Clones Associated with Disease-resistant Genes%高覆盖率水稻BAC库的构建及抗病基因相关克隆的筛选

    Institute of Scientific and Technical Information of China (English)

    王文明; 江光怀; 王世全; 朱立煌; 翟文学

    2001-01-01

    A BAC library for IRBB56, an accession pyramiding Xa4, xa5 and xa13 three bacterial blight resistance genes, was constructed. The library contains 55 296 clones with an average insert size of 132kb. Based on a haploid genome size of 450Mb, the coverage of the library was about 14 genome equivalents that make it oneof the most comprehensive BAC libraries available in rice and provide 99.99% possibility to isolate any interested rice genes or sequences in the library. To determine the representation of organelle DNA homologues in the library, the library was screened with three different choloroplast genes and four mitochondrial genes, respectively. Results from this screening showed that less than 1% of clones in the library contain organelle genomic DNA homologues. Then, DNA markers on three different chromosomes linked to Xa4, xa5, and xa13, and a PCR fragment of rice UROD gene, were used to screen the library resulting in a range of 11~106 hits that will promote the isolation of these genes. The deep coverage and the large insert size of the library will facilitate physical mapping, isolation, and cloning of rice genes.%利用含Xa4、xa5和xa13 3个水稻白叶枯病抗性基因的累加系IRBB56构建了一个水稻细菌人工染色体文库。该文库包含55 296个克隆,平均插入片段为132kb。按水稻基因组为450Mb 计,该文库覆盖14倍基因组,筛选出任一水稻基因或序列的概率为99.99%。用均匀分布的3个叶绿体基因和4个线粒体基因克隆作探针筛选文库,结果显示该文库中含细胞器基因组DNA同源序列的克隆数小于1%。用分布于水稻3条不同染色体、分别与Xa4、xa5和xa13连锁的DNA标记筛选文库,分别检测出11~106个阳性克隆,为克隆这些基因打下了基础。 该文库对水稻基因组的高度覆盖率和较大的插入片段,非常适合于物理作图和基因的分离和克隆。

  11. Primary study on bacterial artificial chromosome (BAC) genomic library of Shaneng milk goats%奶山羊细菌人工染色体基因文库构建的初步研究

    Institute of Scientific and Technical Information of China (English)

    鞠辉明; 张信军; 李贵阳; 刘宗平; 成勇

    2006-01-01

    细菌人工染色体(BAC)文库是进行动物基因组学研究的一个重要手段,为配合山羊乳腺生物反应器的研究,对构建山羊基因组BAC文库进行了初步研究.用HindⅢ酶对莎能奶山羊基因组进行部分酶切,用CHEF (箝位匀强电场)二次电泳法回收150 kb大小条带,电透析回收高分子量(HMW) DNA;同时以BAC载体pBeloBAC11为材料,分别采用限制性内切酶HindⅢ和HK脱磷酶对其进行酶切和脱磷,将该载体自连并通过凝胶回收线性段DNA,获得了可用于构建BAC文库的线性载体.将HMW与载体进行连接,电击转化感受态DH10β大肠杆菌,一次转化得到近2000个克隆.插入片段大小为50~200 kb,基本满足建库的需要.

  12. A Comparative BAC map for the gilthead sea bream (Sparus aurata L.).

    Science.gov (United States)

    Kuhl, Heiner; Sarropoulou, Elena; Tine, Mbaye; Kotoulas, Georgios; Magoulas, Antonios; Reinhardt, Richard

    2011-01-01

    This study presents the first comparative BAC map of the gilthead sea bream (Sparus aurata), a highly valuated marine aquaculture fish species in the Mediterranean. High-throughput end sequencing of a BAC library yielded 92,468 reads (60.6  Mbp). Comparative mapping was achieved by anchoring BAC end sequences to the three-spined stickleback (Gasterosteus aculeatus) genome. BACs that were consistently ordered along the stickleback chromosomes accounted for 14,265 clones. A fraction of 5,249 BACs constituted a minimal tiling path that covers 73.5% of the stickleback chromosomes and 70.2% of the genes that have been annotated. The N50 size of 1,485 "BACtigs" consisting of redundant BACs is 337,253 bp. The largest BACtig covers 2.15 Mbp in the stickleback genome. According to the insert size distribution of mapped BACs the sea bream genome is 1.71-fold larger than the stickleback genome. These results represent a valuable tool to researchers in the field and may support future projects to elucidate the whole sea bream genome.

  13. A Comparative BAC Map for the Gilthead Sea Bream (Sparus aurata L.

    Directory of Open Access Journals (Sweden)

    Heiner Kuhl

    2011-01-01

    Full Text Available This study presents the first comparative BAC map of the gilthead sea bream (Sparus aurata, a highly valuated marine aquaculture fish species in the Mediterranean. High-throughput end sequencing of a BAC library yielded 92,468 reads (60.6 Mbp. Comparative mapping was achieved by anchoring BAC end sequences to the three-spined stickleback (Gasterosteus aculeatus genome. BACs that were consistently ordered along the stickleback chromosomes accounted for 14,265 clones. A fraction of 5,249 BACs constituted a minimal tiling path that covers 73.5% of the stickleback chromosomes and 70.2% of the genes that have been annotated. The N50 size of 1,485 “BACtigs” consisting of redundant BACs is 337,253 bp. The largest BACtig covers 2.15 Mbp in the stickleback genome. According to the insert size distribution of mapped BACs the sea bream genome is 1.71-fold larger than the stickleback genome. These results represent a valuable tool to researchers in the field and may support future projects to elucidate the whole sea bream genome.

  14. 我国濒危家养动物细菌人工染色体基因组文库的构建及应用%BAC genomic library construction and appliance of the endangered domestic animals in China

    Institute of Scientific and Technical Information of China (English)

    丁鸿; 关伟军; 马月辉

    2004-01-01

    细菌人工染色体(BAC)是新近发展起来的一种载体系统,具有克隆容量大,遗传特性稳定,转化效率高,易于操作等优点.在基因组文库构建和基因的快速克隆等方面已得到广泛应用.濒危家养动物细菌人工染色体基因组文库构建的目的在于有效、永久保存和利用其基因资源、快速克隆重要经济和抗逆性状基因以及为广泛、深入开展分子生物学研究提供宝贵的材料.

  15. Data Management inside the Library: Assessing Electronic Resources Data Using the Data Asset Framework Methodology

    Science.gov (United States)

    Ogier, Andi; Hall, Monena; Bailey, Annette; Stovall, Connie

    2014-01-01

    Rapidly growing within academic libraries, library data services have often been focused on assessing research trends and building partnerships outside the library. There are distinct benefits, however, to using data audit methodologies created for these external assessments of researcher practices inside the library as well. In this article, we…

  16. "The Open Library at AU" (Athabasca University): Supporting Open Access and Open Educational Resources

    Science.gov (United States)

    Elliott, Colin; Fabbro, Elaine

    2015-01-01

    To address challenges that learners, course creators, librarians and academics involved with OER and MOOCs are facing when looking for scholarly materials, Athabasca University Library has initiated the development of "the Open Library at AU." This open library is a full library website that provides easy access to open and free…

  17. Library Resources for the Blind and Physically Handicapped: A Directory with FY 1994 Statistics on Readership, Circulation, Budget, Staff, and Collections.

    Science.gov (United States)

    Library of Congress, Washington, DC. National Library Service for the Blind and Physically Handicapped.

    The first part of this directory of National Library Service (NLS) resources for the blind and physically handicapped lists NLS network libraries and machine-lending agencies, arranged alphabetically by state. The information for each library includes its address, telephone and fax numbers, librarian, hours of operation, book and special…

  18. Public Library Clients Prefer Formal Classes for Initial Training on Library’s Online Resources and Informal, On-Demand Assistance for Further Training. A Review of: Ruthven, J. (2010. Training needs and preferences of adult public library clients in the use of online resources. The Australian Library Journal, 59(3, 108-117.

    Directory of Open Access Journals (Sweden)

    Diana K. Wakimoto

    2012-03-01

    Full Text Available Objective – To discover public library clients’ needs and preferences for modes of training on the use of the Internet and the libraries’ online resources and to apply these findings to improve training offered by public library staff.Design – Multiple exploratory case study.Setting – Two public libraries in New South Wales, Australia: a regional library (Mudgee Branch of the Mid-Western Regional Council Library Service and a metropolitan library (Marrickville Central Library.Subjects – A total of 24 public library clients. The participants were split evenly between the two libraries, with 12 from the Mudgee Branch and 12 from the Marrickville Central. The respondents were further subdivided into two groups based on age (35 to 44 years old and 65 or older and evenly distributed by sex within the groups.Methods – This study used naturalistic inquiry to frame the multiple exploratory case study of two public libraries. Ruthven used maximum variation sampling to guide the selection of participants. Library staff helped the researcher to identify possible participants at Marrickville, while the researcher advertised for participants at Mudgee Library and at an Internet/database course taught at the Mudgee Business Enterprise Centre. She used snowball sampling to find additional participants at both sites. Ruthven conducted semi-structured interviews with the participants, with questions covering their preferences, recommendations, and needs for online resource training. The data from the interviews and search logs were analyzed using inductive data analysis.Main Results – Participants preferred small group, face-to-face, formalized instruction for initial training on online resources. For further training, participants preferred individualized assistance and immediate support instead of formal classes. They noted a lack of training opportunities and a lack of help from library staff as sources of frustration when trying to learn to

  19. Scholars' Satisfaction with Digital Library Collection and Gaps in the Provision of Effective Information Resources and Services: A Pakistani Perspective

    Science.gov (United States)

    Khan, Amjid; Ahmed, Shamshad; Masrek, Mohamad Noorman

    2014-01-01

    The study aims to explore the researchers' satisfaction with digital library resources and services and how they improved the research culture in Pakistani universities. A descriptive survey method was employed to achieve objectives of this study. Using stratified random sampling, for this survey we selected 14 public sector universities of Khyber…

  20. The Availability and Utilization of School Library Resources in Some Selected Secondary Schools (High School) in Rivers State

    Science.gov (United States)

    Owate, C. N.; Iroha, Okpa

    2013-01-01

    This study investigates the availability and utilization of school library resources by Secondary School (High School) Students. Eight Selected Secondary Schools in Rivers State, Nigeria were chosen based on their performance in external examinations and geographic locations. In carrying out the research, questionnaires were administered to both…

  1. Impact of Electronic Resources and Usage in Academic Libraries in Ghana: Evidence from Koforidua Polytechnic & All Nations University College, Ghana

    Science.gov (United States)

    Akussah, Maxwell; Asante, Edward; Adu-Sarkodee, Rosemary

    2015-01-01

    The study investigates the relationship between impact of electronic resources and its usage in academic libraries in Ghana: evidence from Koforidua Polytechnic & All Nations University College, Ghana. The study was a quantitative approach using questionnaire to gather data and information. A valid response rate of 58.5% was assumed. SPSS…

  2. Extending Geant4 Parallelism with External Libraries (MPI, TBB) and Its Use on HPC Resources

    CERN Document Server

    Dotti, Andrea; Barrand, Guy; Hrivnacova, Ivana; Murakami, Koichi

    2016-01-01

    With Geant4 Version 10.0, released in December 2013, one of the most widely used Monte-Carlo codes has been ported to take full advantage of multi- and many-core CPUs thanks to the introduction of event-level parallelism via multithreading. In this paper we review recent developments to allow for a better integration of parallel Geant4 jobs with external libraries. We have chosen to develop examples using the popular Intel Threading Building Block (for short TBB) as an alternative parallelization approach to the native Geant4 POSIX. To simplify the scaling of a Geant4 application across nodes on a cluster we are improving the support of MPI in Geant4. In particular it is now possible to run an hybrid MPI/MT application that uses MPI to scale across nodes and MT to scale across cores. %The recent developments allow users to easily implement parallel application resources that scale on a very large number of nodes and cores typical of HPC resources.

  3. A Comparative Analysis of the Availability of Information Resources on Ibibio Culture in the University of Uyo and Akwa Ibom State Public Library

    Science.gov (United States)

    Okon, Henry Itohowo; Simon, Jehu S.; Akai, Iniobong

    2015-01-01

    This study reports the results of a survey of the available holdings of information resources on Ibibio culture in the University of Uyo Library and Akwa Ibom State Library. The specific objectives of the study were to determine the different size of information resources on funeral, fattening (Mbobo), taboos, myths as well as dissemination in the…

  4. Central New York Library Resources Council CLRC Regional Digitization Plan. Final Report for the Preparing Central New York History for the Future LSTA Project.

    Science.gov (United States)

    Sywetz, Betsy

    The primary goal for digitization projects sponsored by the Central New York Library Resources Council (CLRC) is enhanced access for the people of the region to digital resources created from collections in Central New York's libraries, archives and museums. The CLRC Digitization Plan provides a framework for the support of digitization activities…

  5. GeoDeepDive: Towards a Machine Reading-Ready Digital Library and Information Integration Resource

    Science.gov (United States)

    Husson, J. M.; Peters, S. E.; Livny, M.; Ross, I.

    2015-12-01

    Recent developments in machine reading and learning approaches to text and data mining hold considerable promise for accelerating the pace and quality of literature-based data synthesis, but these advances have outpaced even basic levels of access to the published literature. For many geoscience domains, particularly those based on physical samples and field-based descriptions, this limitation is significant. Here we describe a general infrastructure to support published literature-based machine reading and learning approaches to information integration and knowledge base creation. This infrastructure supports rate-controlled automated fetching of original documents, along with full bibliographic citation metadata, from remote servers, the secure storage of original documents, and the utilization of considerable high-throughput computing resources for the pre-processing of these documents by optical character recognition, natural language parsing, and other document annotation and parsing software tools. New tools and versions of existing tools can be automatically deployed against original documents when they are made available. The products of these tools (text/XML files) are managed by MongoDB and are available for use in data extraction applications. Basic search and discovery functionality is provided by ElasticSearch, which is used to identify documents of potential relevance to a given data extraction task. Relevant files derived from the original documents are then combined into basic starting points for application building; these starting points are kept up-to-date as new relevant documents are incorporated into the digital library. Currently, our digital library stores contains more than 360K documents supplied by Elsevier and the USGS and we are actively seeking additional content providers. By focusing on building a dependable infrastructure to support the retrieval, storage, and pre-processing of published content, we are establishing a foundation for

  6. BAC-HAPPY mapping (BAP mapping): a new and efficient protocol for physical mapping.

    Science.gov (United States)

    Vu, Giang T H; Dear, Paul H; Caligari, Peter D S; Wilkinson, Mike J

    2010-02-08

    Physical and linkage mapping underpin efforts to sequence and characterize the genomes of eukaryotic organisms by providing a skeleton framework for whole genome assembly. Hitherto, linkage and physical "contig" maps were generated independently prior to merging. Here, we develop a new and easy method, BAC HAPPY MAPPING (BAP mapping), that utilizes BAC library pools as a HAPPY mapping panel together with an Mbp-sized DNA panel to integrate the linkage and physical mapping efforts into one pipeline. Using Arabidopsis thaliana as an exemplar, a set of 40 Sequence Tagged Site (STS) markers spanning approximately 10% of chromosome 4 were simultaneously assembled onto a BAP map compiled using both a series of BAC pools each comprising 0.7x genome coverage and dilute (0.7x genome) samples of sheared genomic DNA. The resultant BAP map overcomes the need for polymorphic loci to separate genetic loci by recombination and allows physical mapping in segments of suppressed recombination that are difficult to analyze using traditional mapping techniques. Even virtual "BAC-HAPPY-mapping" to convert BAC landing data into BAC linkage contigs is possible.

  7. Sequencing of BAC pools by different next generation sequencing platforms and strategies

    Directory of Open Access Journals (Sweden)

    Scholz Uwe

    2011-10-01

    Full Text Available Abstract Background Next generation sequencing of BACs is a viable option for deciphering the sequence of even large and highly repetitive genomes. In order to optimize this strategy, we examined the influence of read length on the quality of Roche/454 sequence assemblies, to what extent Illumina/Solexa mate pairs (MPs improve the assemblies by scaffolding and whether barcoding of BACs is dispensable. Results Sequencing four BACs with both FLX and Titanium technologies revealed similar sequencing accuracy, but showed that the longer Titanium reads produce considerably less misassemblies and gaps. The 454 assemblies of 96 barcoded BACs were improved by scaffolding 79% of the total contig length with MPs from a non-barcoded library. Assembly of the unmasked 454 sequences without separation by barcodes revealed chimeric contig formation to be a major problem, encompassing 47% of the total contig length. Masking the sequences reduced this fraction to 24%. Conclusion Optimal BAC pool sequencing should be based on the longest available reads, with barcoding essential for a comprehensive assessment of both repetitive and non-repetitive sequence information. When interest is restricted to non-repetitive regions and repeats are masked prior to assembly, barcoding is non-essential. In any case, the assemblies can be improved considerably by scaffolding with non-barcoded BAC pool MPs.

  8. Preference and Use of Electronic Information and Resources by Blind/Visually Impaired in NCR Libraries in India

    Directory of Open Access Journals (Sweden)

    Shailendra Kumar

    2013-06-01

    Full Text Available This paper aims to determine the preference and use of electronic information and resources by blind/visually impaired users in the leading National Capital Region (NCR libraries of India. Survey methodology has been used as the basic research tool for data collection with the help of questionnaires. The 125 in total users surveyed in all the five libraries were selected randomly on the basis of willingness of the users with experience of working in digital environments to participate in the survey. The survey results were tabulated and analyzed with descriptive statistics methods using Excel software and 'Stata version 11'. The findings reveal that ICT have a positive impact in the lives of people with disabilities as it helps them to work independently and increases the level of confidence among them. The Internet is the most preferred medium of access to information among the majority of blind/visually impaired users. The 'Complexity of content available on the net' is found as the major challenge faced during Internet use by blind users of NCR libraries. 'Audio books on CDs/DVDs and DAISY books' are the most preferred electronic resources among the majority of blind/visually impaired users. This study will help the library professionals and organizations/institutions serving people with disabilities to develop effective library services for blind/visually impaired users in the digital environment on the basis of findings on information usage behavior in the study.

  9. Not an Inexhaustible Resource: Valuation and Depreciation of Library Collections in the Queensland Department of Education.

    Science.gov (United States)

    Cram, Jennifer

    1997-01-01

    Anecdotal evidence suggests that valuation of library collections is not being addressed by library managers, despite the growing popularity of accrual accounting in publicly funded institutions. This article discusses the implications of asset valuation and describes the development and implementation a method for valuing the library collections…

  10. Electronic Resource Expenditure and the Decline in Reference Transaction Statistics in Academic Libraries

    Science.gov (United States)

    Dubnjakovic, Ana

    2012-01-01

    The current study investigates factors influencing increase in reference transactions in a typical week in academic libraries across the United States of America. Employing multiple regression analysis and general linear modeling, variables of interest from the "Academic Library Survey (ALS) 2006" survey (sample size 3960 academic libraries) were…

  11. Cochrane图书馆针灸资源述评%Acupuncture resources in Cochrane Library

    Institute of Scientific and Technical Information of China (English)

    刘迈兰; 兰蕾; 吴曦; 杜怀斌; 唐宏智; 梁繁荣

    2011-01-01

    计算机检索Cochrane Library(CL)中6个数据库收录的针灸资源:系统评价数据库共72篇,12篇文献结论支持针灸疗效确切,29篇针灸疗效不确定,1篇不支持针灸治疗癫痫,31篇为计划书;疗效评价文摘数据库共121篇,涉及疾病或症状种类多且形式丰富;对照试验注册中心数据库共4218篇针灸随机对照和临床对照试验文献;方法学注册数据库共43篇,研究主要集中于盲法、研究的方法学质量评估和发表偏倚等;卫生技术评估数据库与经济学评估数据库分别检索到25篇、18篇,多以针刺镇痛为研究对象.结果表明CL收集的针灸资源丰富、分类详细、证据级别较高,是针灸临床研究证据的良好资源之一.%To identify acupuncture resources in six databases of Cochrane Library (CL) with computer retrieve. Seventy-two literatures were identified in Cochrane Database of Systematic Reviews (CDSR). Among them, 12 Cochrane systematic review (CSR) verified the effectiveness of acupuncture, 29 concerning the indeterminacy of the efficacy of acupuncture with 1 didn't support acupuncture for epilepsy and 31 remained as protocols; 121 literatures were found in Database of Abstracts of Reviews of Effects (DARE) with more types of diseases or symptoms and rich modality comparing to CSR; 4218 randomized controlled trials and clinical controlled trials were identified in Cochrane Central Register of Controlled Trials (CCRCT); 43 literatures in Cochrane Methodology Register Database (CMRD) which focused on blindness study, quality assessment of methodology of research and publication bias and so on; 25 literatures in Health Technology Assessment Database (HTAD) and 18 in NHS Economic Evaluation Database (NHS EED) which were centered on acupuncture analgesia. Consequently, acupuncture literatures in 6 databases of CL do provide good resources for acupuncture researchers due to its abundant content,concrete classification and high quality evidence.

  12. Comparative BAC-based mapping in the white-throated sparrow, a novel behavioral genomics model, using interspecies overgo hybridization

    Directory of Open Access Journals (Sweden)

    Gonser Rusty A

    2011-06-01

    Full Text Available Abstract Background The genomics era has produced an arsenal of resources from sequenced organisms allowing researchers to target species that do not have comparable mapping and sequence information. These new "non-model" organisms offer unique opportunities to examine environmental effects on genomic patterns and processes. Here we use comparative mapping as a first step in characterizing the genome organization of a novel animal model, the white-throated sparrow (Zonotrichia albicollis, which occurs as white or tan morphs that exhibit alternative behaviors and physiology. Morph is determined by the presence or absence of a complex chromosomal rearrangement. This species is an ideal model for behavioral genomics because the association between genotype and phenotype is absolute, making it possible to identify the genomic bases of phenotypic variation. Findings We initiated a genomic study in this species by characterizing the white-throated sparrow BAC library via filter hybridization with overgo probes designed for the chicken, turkey, and zebra finch. Cross-species hybridization resulted in 640 positive sparrow BACs assigned to 77 chicken loci across almost all macro-and microchromosomes, with a focus on the chromosomes associated with morph. Out of 216 overgos, 36% of the probes hybridized successfully, with an average number of 3.0 positive sparrow BACs per overgo. Conclusions These data will be utilized for determining chromosomal architecture and for fine-scale mapping of candidate genes associated with phenotypic differences. Our research confirms the utility of interspecies hybridization for developing comparative maps in other non-model organisms.

  13. Rationalization of the investment on information resources in libraries: a case study at the Library of the University of Almeria (Spain

    Directory of Open Access Journals (Sweden)

    Encarna Fuentes Melero

    2013-06-01

    Full Text Available The economic situation Europe is currently experiencing, and consequently, the constant economic adjustments set by Public Administrations of European countries, are forcing the university libraries to rethink their acquisitions policies under the criteria of use and cost, in order to achieve a greater efficiency in terms of offered services, available resources and invested budget. The aim of this paper is to describe how the Library of the University of Almeria (Spain is acting in this environment, producing annual assessment reports of its information resources, and using them as the basis for raising its budget. This evaluation includes both the electronic resources (through detailed studies of the number of searches and downloaded documents in all subscribed e-journals and e-books databases and the recommended bibliography for students in printed format (through the analysis of the number of loans and the use in the reading rooms. As a result of this analysis, adaptation of the bibliographic collection to the users’ needs, modification of the staff workloads, and accuracy of the librarians training programs, have been achieved.

  14. Keeping Libraries Relevant in the Semantic Web with RDA: Resource Description and Access

    Directory of Open Access Journals (Sweden)

    Barbara Tillett

    2011-12-01

    Full Text Available Catalogare non vuol dire semplicemente costruire un catalogo. Vuol dire far sì che gli utenti accedano tempestivamente alle informazioni pertinenti alle loro esigenze. Il lavoro di identificazione delle risorse raccolte da biblioteche, archivi, musei, dà luogo a ricchi metadati che possono essere riutilizzati per molti scopi ("le attività dell’utente". Ciò comporta la descrizione delle risorse e il mostrare le loro relazioni con persone, famiglie, enti e altre risorse, consentendo così agli utenti di navigare attraverso surrogati delle risorse per ottenere più rapidamente le informazioni di cui hanno bisogno. I metadati costruiti lungo tutto il ciclo di vita di una risorsa sono particolarmente preziosi per molti tipi di utenti: dai creatori delle risorse, agli editori, alle agenzie, ai librai, agli aggregatori di risorse, ai fornitori di sistemi, alle biblioteche, ad altre istituzioni culturali ed agli utenti finali. Il nuovo codice internazionale di catalogazione, RDA: Resource Description e Access, è progettato per soddisfare le attività di base degli utenti producendo metadati ben formati e interconnessi per l'ambiente digitale, dando la possibilità alle biblioteche di rimanere rilevanti nel web semantico. Acknowledge Il testo inglese del saggio è pubblicato in "Serials", November 2011, 24, 3, con il titolo Keeping Libraries Relevant in the Semantic Web with RDA: Resource Description and Access, DOI: http://dx.doi.org/10.1629/24266. Traduzione italiana di Maria Chiara Iorio e Tiziana Possemato, che ringraziano Carlo Bianchini e Mauro Guerrini per la rilettura della traduzione.

  15. Herpesvirus BACs: past, present, and future.

    Science.gov (United States)

    Warden, Charles; Tang, Qiyi; Zhu, Hua

    2011-01-01

    The herpesviridae are a large family of DNA viruses with large and complicated genomes. Genetic manipulation and the generation of recombinant viruses have been extremely difficult. However, herpesvirus bacterial artificial chromosomes (BACs) that were developed approximately 10 years ago have become useful and powerful genetic tools for generating recombinant viruses to study the biology and pathogenesis of herpesviruses. For example, BAC-directed deletion mutants are commonly used to determine the function and essentiality of viral genes. In this paper, we discuss the creation of herpesvirus BACs, functional analyses of herpesvirus mutants, and future applications for studies of herpesviruses. We describe commonly used methods to create and mutate herpesvirus BACs (such as site-directed mutagenesis and transposon mutagenesis). We also evaluate the potential future uses of viral BACs, including vaccine development and gene therapy.

  16. Herpesvirus BACs: Past, Present, and Future

    Directory of Open Access Journals (Sweden)

    Charles Warden

    2011-01-01

    Full Text Available The herpesviridae are a large family of DNA viruses with large and complicated genomes. Genetic manipulation and the generation of recombinant viruses have been extremely difficult. However, herpesvirus bacterial artificial chromosomes (BACs that were developed approximately 10 years ago have become useful and powerful genetic tools for generating recombinant viruses to study the biology and pathogenesis of herpesviruses. For example, BAC-directed deletion mutants are commonly used to determine the function and essentiality of viral genes. In this paper, we discuss the creation of herpesvirus BACs, functional analyses of herpesvirus mutants, and future applications for studies of herpesviruses. We describe commonly used methods to create and mutate herpesvirus BACs (such as site-directed mutagenesis and transposon mutagenesis. We also evaluate the potential future uses of viral BACs, including vaccine development and gene therapy.

  17. Defining an open access resource strategy for research libraries:Part Ⅱ——Challenges and responses

    Institute of Scientific and Technical Information of China (English)

    Xiaolin; ZHANG; Yan; ZENG; Lin; LI; Xiwen; LIU

    2012-01-01

    Purpose:This paper aims to provide a systematic analysis of challenges and responses of the imminent open access-based environment so as to enable sound development of open access(OA)resource strategies.Design/methodology/approach:A systematic and evidence-based analysis is used to discover,identify,and contextualize the factors that relate or interact.A challenge metric for the overall context and additional frameworks or alternative lists is used for the detailed analysis.Findings:Research libraries are facing a reality conflict between the traditional library solution and the OA solution.New resource strategies are needed but have to be based on an open knowledge infrastructure and a systematic analysis of factors and alternatives.Support for OA publishing is becoming a major task and complicated capabilities involved call for libraries to play a central role.Hybrid collection development should be in place to take full advantages of the open world while ensuring reliable and trusted retrieval,reuse,and preservation.Research limitations:The research covers main developments in US and European countries mostly in scientific fields and for scholarly papers.Practical implications:There are already evidences for sound OA resource policies.With the imminent dominance of OA resources,research libraries should and can set up their OA resource strategies.Originality/value:The paper suggests a factor metric as an analysis framework,presents rationale lists and policies to support OA publishing,and proposes a hybrid collection strategy to plan for collection development,retrieval,and preservation.

  18. Innovation on hospital library human resources management%医院图书馆人力资源管理创新

    Institute of Scientific and Technical Information of China (English)

    王玉双

    2014-01-01

    Based on the problems which can described as backward management of human resources, librarian quality, brain drain and irrational human resources structure of human resources management in hospital library, new concept of hospital library human resources management is presented. Suggestions such as implementing scientific human resources management and training, post appointment and performance system and making human resources management policy are also discussed.%结合制约医院图书馆发展中人力资源管理的管理相对滞后、馆员本身的问题、人才流失严重、人才结构不合理等因素,提出了医院图书馆人力资源管理的新理念,阐述了实施科学人才管理、人才培训、队伍建设以及建立绩效考核制度、岗位聘用制度、人才政策体系等建议。

  19. Electronic resources at the University of Sharjah medical library: an investigation of students' information-seeking behavior.

    Science.gov (United States)

    Boumarafi, Behdja

    2010-10-01

    Electronic information is becoming prevalent worldwide, and its use is growing exponentially as more and more users are recognizing the potential that it offers in terms of access and delivery. However, with the introduction of new tools for e-information searching and retrieval, users have to readjust their information-seeking behavior to cope with the corresponding changes. The University of Sharjah library is steadily increasing its investment in e-resources to offer ubiquitous access to the growing body of literature in areas that interest the community it serves. This study reports the findings of a survey conducted to investigate the information-seeking behavior of medical students at the medical library. Results showed evidence of use of e-resources, but they did not explicitly establish that some of the major problems mentioned by participants did hinder the information searches of the respondents. An extensive literature review sets the background for the study.

  20. News from the Library: Online particle physics information: a unique compilation of information resources in particle physics

    CERN Multimedia

    CERN Library

    2012-01-01

    Are you looking for some specific information in particle physics? For example, the main literature databases, data repositories or laboratories...   Just go to the Libary's Online Particle Physics Information page. There you'll find a wide selection of relevant information, as well as resources in particle physics and related areas. The collection covers all aspects of the discipline - in addition to traditional scientific information resources you can find, for example, a selection of relevant blogs and art websites. This webpage is an extended and regularly updated version of the chapter on Online Particle Physics Information in the Review of Particle Properties. It is maintained by the CERN Library team which welcomes suggestions for additions and updates: library.desk@cern.ch.  

  1. Development of one set of chromosome-specific microsatellite-containing BACs and their physical mapping in Gossypium hirsutum L.

    Science.gov (United States)

    Wang, Kai; Guo, Wangzhen; Zhang, Tianzhen

    2007-09-01

    Fluorescence in situ hybridization (FISH), using bacterial artificial chromosome (BAC) clone as probe, is a reliable cytological technique for chromosome identification. It has been used in many plants, especially in those containing numerous small chromosomes. We previously developed eight chromosome-specific BAC clones from tetraploid cotton, which were used as excellent cytological markers for chromosomes identification. Here, we isolated the other chromosome-specific BAC clones to make a complete set for the identification of all 26 chromosome-pairs by this technology in tetraploid cotton (Gossypium hirsutum L.). This set of BAC markers was demonstrated to be useful to assign each chromosome to a genetic linkage group unambiguously. In addition, these BAC clones also served as convenient and reliable landmarks for establishing physical linkage with unknown targeted sequences. Moreover, one BAC containing an EST, with high sequence similarity to a G. hirsutum ethylene-responsive element-binding factor was located physically on the long arm of chromosome A7 with the help of a chromosome-A7-specific BAC FISH marker. Comparative analysis of physical marker positions in the chromosomes by BAC-FISH and genetic linkage maps demonstrated that most of the 26 BAC clones were localized close to or at the ends of their respective chromosomes, and indicated that the recombination active regions of cotton chromosomes are primarily located in the distal regions. This technology also enables us to make associations between chromosomes and their genetic linkage groups and re-assign each chromosome according to the corresponding genetic linkage group. This BAC clones and BAC-FISH technology will be useful for us to evaluate grossly the degree to which a linkage map provides adequate coverage for developing a saturated genetic map, and provides a powerful resource for cotton genomic researches.

  2. Intra- and interchromosomal rearrangements between cowpea [Vigna unguiculata (L.) Walp.] and common bean (Phaseolus vulgaris L.) revealed by BAC-FISH.

    Science.gov (United States)

    Vasconcelos, Emanuelle Varão; de Andrade Fonsêca, Artur Fellipe; Pedrosa-Harand, Andrea; de Andrade Bortoleti, Kyria Cilene; Benko-Iseppon, Ana Maria; da Costa, Antônio Félix; Brasileiro-Vidal, Ana Christina

    2015-06-01

    Cowpea (Vigna unguiculata) is an annual legume grown in tropical and subtropical regions, which is economically relevant due to high protein content in dried beans, green pods, and leaves. In this work, a comparative cytogenetic study between V. unguiculata and Phaseolus vulgaris (common bean) was conducted using BAC-FISH. Sequences previously mapped in P. vulgaris chromosomes (Pv) were used as probes in V. unguiculata chromosomes (Vu), contributing to the analysis of macrosynteny between both legumes. Thirty-seven clones from P. vulgaris 'BAT93' BAC library, corresponding to its 11 linkage groups, were hybridized in situ. Several chromosomal rearrangements were identified, such as translocations (between BACs from Pv1 and Pv8; Pv2 and Pv3; as well as Pv2 and Pv11), duplications (BAC from Pv3), as well as paracentric and pericentric inversions (BACs from Pv3, and Pv4, respectively). Two BACs (from Pv2 and Pv7), which hybridized at terminal regions in almost all P. vulgaris chromosomes, showed single-copy signal in Vu. Additionally, 17 BACs showed no signal in V. unguiculata chromosomes. The present results demonstrate the feasibility of using BAC libraries in comparative chromosomal mapping and karyotype evolution studies between Phaseolus and Vigna species, and revealed several macrosynteny and collinearity breaks among both legumes.

  3. Redefining the Facilities of Community College Libraries and Learning Resource Centers in the 21st Century

    Science.gov (United States)

    Simon, Matthew J.

    2004-01-01

    The decision to build a new or renovate an existing community college library has been triggered by one or more of these problems: collection growth, increased student population, or new technologies. Increasingly, the perception of diminished use is triggering reconfiguration of library space. This article discusses implications, case studies,…

  4. Equalizing Access to Electronic Networked Resources: A Model for Rural Libraries in the United States.

    Science.gov (United States)

    Senkevitch, Judith J.; Wolfram, Dietmar

    1994-01-01

    Provides an overview of the current state of networking technology in rural libraries and describes a model for educating rural librarians in accessing electronic networks. Topics discussed include information needs in rural libraries; telecommunications technology access in rural areas; and examples of services to enhance information access.…

  5. Wikipedia Lover, Not a Hater: Harnessing Wikipedia to Increase the Discoverability of Library Resources

    Science.gov (United States)

    Elder, Danielle; Westbrook, R. Niccole; Reilly, Michele

    2012-01-01

    During the spring of 2010, the University of Houston Libraries Digital Services Department began an initiative to promote existing and upcoming collections in the University of Houston Digital Library and drive traffic to the online repository. Spurred by an OCLC report (De Rosa et al. 2005) that only two percent of college and university students…

  6. A Century of Change: The Evolution of School Library Resources, 1915-2015

    Science.gov (United States)

    Lamb, Annette

    2015-01-01

    School libraries have been in existence since at least the eighth century. However, it wasn't until the twentieth century that the school library was seen primarily as "a source of enrichment for the curriculum, and a means of developing reading and study habits in the pupils" (Clyde 1981, 263). While the formats available and tools for…

  7. Marketing the Virtual Library

    Science.gov (United States)

    Fagan, Jody Condit

    2009-01-01

    Far more people are familiar with their local public or college library facility than their library's website and online resources. In fact, according to a recent survey, 96% of Americans said they had visited a library in person, but less than one-third have visited their online library. Since everyone agrees that online library resources are…

  8. Defining an open access resource strategy for research libraries: Part Ⅰ—The coming main streamlization of open access resources?

    Institute of Scientific and Technical Information of China (English)

    Xiaolin; ZHANG; Lin; LI; Xiwen; LIU; Yan; ZENG

    2011-01-01

    Open access(OA)resources are becoming a main part of scholarly information.The paper summarizes the fast growth of OA journals and OA repositories,the outstanding OA journal impact increase,the rise of mega-OA journals like PLoS ONE,the overwhelming support of research communities to open access of publicly funded research,and the quick building up of OA support structures.These forces work together to push scholarly communications toward a turning point where OA resources occupy a major and increasing share of scholarly research resources and when management of OA resources becomes a strategic,yet unfamiliar,and potentially disruptive task for research libraries.This paper is composed of two parts.The first part will focus on the development of OA resources,while the second part will analyze the challenges in management of OA resources and develop an OA resource strategy exemplified by some of NSL’s initiatives.

  9. BAC-end sequences analysis provides first insights into coffee (Coffea canephora P.) genome composition and evolution

    OpenAIRE

    Dereeper, Alexis; Guyot, Romain; Dubreuil Tranchant, Christine; Anthony, François; Argout, X.; Bellis, F; Combes, Marie-Christine; Gavory, F.; De Kochko, Alexandre; Kudrna, D.; Leroy, T.; Poulain, J.; Rondeau, M.; Song, X; Wing, R

    2013-01-01

    Coffee is one of the world's most important agricultural commodities. Coffee belongs to the Rubiaceae family in the euasterid I clade of dicotyledonous plants, to which the Solanaceae family also belongs. Two bacterial artificial chromosome (BAC) libraries of a homozygous doubled haploid plant of Coffea canephora were constructed using two enzymes, HindIII and BstYI. A total of 134,827 high quality BAC-end sequences (BESs) were generated from the 73,728 clones of the two libraries, and 131,41...

  10. A BAC-based physical map of Zhikong scallop (Chlamys farreri Jones et Preston.

    Directory of Open Access Journals (Sweden)

    Xiaojun Zhang

    Full Text Available Zhikong scallop (Chlamys farreri is one of the most economically important aquaculture species in China. Physical maps are crucial tools for genome sequencing, gene mapping and cloning, genetic improvement and selective breeding. In this study, we have developed a genome-wide, BAC-based physical map for the species. A total of 81,408 clones from two BAC libraries of the scallop were fingerprinted using an ABI 3130xl Genetic Analyzer and a fingerprinting kit developed in our laboratory. After data processing, 63,641 (∼5.8× genome coverage fingerprints were validated and used in the physical map assembly. A total of 3,696 contigs were assembled for the physical map. Each contig contained an average of 10.0 clones, with an average physical size of 490 kb. The combined total physical size of all contigs was 1.81 Gb, equivalent to approximately 1.5 fold of the scallop haploid genome. A total of 10,587 BAC end sequences (BESs and 167 markers were integrated into the physical map. We evaluated the physical map by overgo hybridization, BAC-FISH (fluorescence in situ hybridization, contig BAC pool screening and source BAC library screening. The results have provided evidence of the high reliability of the contig physical map. This is the first physical map in mollusc; therefore, it provides an important platform for advanced research of genomics and genetics, and mapping of genes and QTL of economical importance, thus facilitating the genetic improvement and selective breeding of the scallop and other marine molluscs.

  11. Information as an Economic Resource: The Role of Public Libraries in Nigeria

    Directory of Open Access Journals (Sweden)

    Adebayo Oyeronke

    2012-12-01

    Full Text Available The development of a country depends greatly on how much information its citizenry are exposed to. Information is so crucial that it has been recognized as the fifth factor of production. Information has no substitute when it comes to the national development because it has been identified as the driver of economic growth and productivity. The paper discussed the roles of public libraries in Nigeria and also examined various ways in which public libraries can help curb unemployment among youths by providing them with timely and accurate information. It concluded that public libraries should identify themselves with the aspiration of economic development of the country.

  12. Inference of subgenomic origin of BACs in an interspecific hybrid sugarcane cultivar by overlapping oligonucleotide hybridizations.

    Science.gov (United States)

    Kim, Changsoo; Robertson, Jon S; Paterson, Andrew H

    2011-09-01

    Sugarcane (Saccharum spp.) breeders in the early 20th century made remarkable progress in increasing yield and disease resistance by crossing Saccharum spontaneum L., a wild relative, to Saccharum officinarum L., a traditional cultivar. Modern sugarcane cultivars have approximately 71%-83% of their chromosomes originating from S. officinarum, approximately 10%-21% from S. spontaneum, and approximately 2%-13% recombinant or translocated chromosomes. In the present work, C(0)t-based cloning and sequencing (CBCS) was implemented to further explore highly repetitive DNA and to seek species-specific repeated DNA in both S. officinarum and S. spontaneum. For putatively species-specific sequences, overlappping oligonucleotide probes (overgos) were designed and hybridized to BAC filters from the interspecific hybrid sugarcane cultivar 'R570' to try to deduce parental origins of BAC clones. We inferred that 12 967 BACs putatively originated from S. officinarum and 5117 BACs from S. spontaneum. Another 1103 BACs were hybridized by both species-specific overgos, too many to account for by conventional recombination, thus suggesting ectopic recombination and (or) translocation of DNA elements. Constructing a low C(0)t library is useful to collect highly repeated DNA sequences and to search for potentially species-specific molecular markers, especially among recently diverged species. Even in the absence of repeat families that are species-specific in their entirety, the identification of localized variations within consensus sequences, coupled with the site specificity of short synthetic overgos, permits researchers to monitor species-specific or species-enriched variants.

  13. BacS: an abundant bacteroid protein in Rhizobium etli whose expression ex planta requires nifA.

    Science.gov (United States)

    Jahn, Olivia J; Davila, Guillermo; Romero, David; Noel, K Dale

    2003-01-01

    Rhizobium etli CFN42 bacteroids from bean nodules possessed an abundant 16-kDa protein (BacS) that was found in the membrane pellet after cell disruption. This protein was not detected in bacteria cultured in tryptone-yeast extract. In minimal media, it was produced at low oxygen concentration but not in a mutant whose nifA was disrupted. N-terminal sequencing of the protein led to isolation of a bacS DNA fragment. DNA hybridization and nucleotide sequencing revealed three copies of the bacS gene, all residing on the main symbiotic plasmid of strain CFN42. A stretch of 304 nucleotides, exactly conserved upstream of all three bacS open reading frames, had very close matches with the NifA and sigma 54 consensus binding sequences. The only bacS homology in the genetic sequence databases was to three hypothetical proteins of unknown function, all from rhizobial species. Mutation and genetic complementation indicated that each of the bacS genes gives rise to a BacS polypeptide. Mutants disrupted or deleted in all three genes did not produce the BacS polypeptide but were Nod+ and Fix+ on Phaseolus vulgaris.

  14. A multiway analysis for identifying high integrity bovine BACs

    OpenAIRE

    McEwan John C; Brauning Rudiger; McWilliam Sean; Barris Wesley; Ratnakumar Abhirami; Snelling Warren M; Dalrymple Brian P

    2009-01-01

    Abstract Background In large genomics projects involving many different types of analyses of bacterial artificial chromosomes (BACs), such as fingerprinting, end sequencing (BES) and full BAC sequencing there are many opportunities for the identities of BACs to become confused. However, by comparing the results from the different analyses, inconsistencies can be identified and a set of high integrity BACs preferred for future research can be defined. Results The location of each bovine BAC in...

  15. 454 sequencing of pooled BAC clones on chromosome 3H of barley

    Directory of Open Access Journals (Sweden)

    Yamaji Nami

    2011-05-01

    Full Text Available Abstract Background Genome sequencing of barley has been delayed due to its large genome size (ca. 5,000Mbp. Among the fast sequencing systems, 454 liquid phase pyrosequencing provides the longest reads and is the most promising method for BAC clones. Here we report the results of pooled sequencing of BAC clones selected with ESTs genetically mapped to chromosome 3H. Results We sequenced pooled barley BAC clones using a 454 parallel genome sequencer. A PCR screening system based on primer sets derived from genetically mapped ESTs on chromosome 3H was used for clone selection in a BAC library developed from cultivar "Haruna Nijo". The DNA samples of 10 or 20 BAC clones were pooled and used for shotgun library development. The homology between contig sequences generated in each pooled library and mapped EST sequences was studied. The number of contigs assigned on chromosome 3H was 372. Their lengths ranged from 1,230 bp to 58,322 bp with an average 14,891 bp. Of these contigs, 240 showed homology and colinearity with the genome sequence of rice chromosome 1. A contig annotation browser supplemented with query search by unique sequence or genetic map position was developed. The identified contigs can be annotated with barley cDNAs and reference sequences on the browser. Homology analysis of these contigs with rice genes indicated that 1,239 rice genes can be assigned to barley contigs by the simple comparison of sequence lengths in both species. Of these genes, 492 are assigned to rice chromosome 1. Conclusions We demonstrate the efficiency of sequencing gene rich regions from barley chromosome 3H, with special reference to syntenic relationships with rice chromosome 1.

  16. Ressourcen für den Personal Digital Assistant (PDA an Bibliotheken / Resources for the Personal Digital Assistant (PDA at libraries

    Directory of Open Access Journals (Sweden)

    Dollfuß, Helmut

    2008-09-01

    Full Text Available Support of PDAs is a matter of course for medical libraries in USA. European medical libraries show interest in this mobile technology but lack knowledge about ways and means to advocate PDA usage. This paper wants to reveal options for offering PDA resources to patrons. Possible ways to foster PDA usage are subsumed in 12 groups along with examples from libraries which published papers about their operating experience with these new technologies. Finally good reasons to start a PDA project and the most appropriate PDA resources and some concerns about PDA usage are listet.

  17. Digital case library: a resource for teaching, learning, and diagnosis support in radiology.

    Science.gov (United States)

    Macura, K J; Macura, R T; Morstad, B D

    1995-01-01

    The authors compiled a digital case library, a database of cases for intracranial masses, that can be used as an electronic teaching file and image source for case-based teaching applications, electronic textbooks, and diagnosis support tools. The library is a relational database with a feature-coded image archive that is structured around relevant radiologic findings. Its index for coding image content is structured as a hierarchical image description index that uses the relational format. Rules that control the search direction within the library and that generate lists of diagnostic hypotheses for decision support tools are embedded within the database structure. Currently, the library consists of 200 cases and 1,100 images that present intracranial masses on radiographs, computed tomograms, magnetic resonance images, and angiograms. Each image in the library is indexed according to its radiologic content. The user may search for reference images that contain particular radiologic features by formulating image content-based queries. The hierarchical index of radiologic findings allows multilevel query formulation that depends on the user's level of experience.

  18. Genome resource for the Indonesian coelacanth, Latimeria menadoensis.

    Science.gov (United States)

    Danke, Joshua; Miyake, Tsutomu; Powers, Thomas; Schein, Jacqueline; Shin, Heesun; Bosdet, Ian; Erdmann, Mark; Caldwell, Roy; Amemiya, Chris T

    2004-03-01

    We have generated a BAC library from the Indonesian coelacanth, Latimeria menadoensis. This library was generated using genomic DNA of nuclei isolated from heart tissue, and has an average insert size of 171 kb. There are a total of 288 384-well microtiter dishes in the library (110,592 clones) and its genomic representation is estimated to encompass > or = 7X coverage based on the amount of DNA presumably cloned in the library as well as via hybridization with probes to a small set of single copy genes. This genomic resource has been made available to the public and should prove useful to the scientific community for many applications, including comparative genomics, molecular evolution and conservation genetics.

  19. A Spectrum of Interoperability: The Site for Science Prototype for the NSDL; Re-Inventing the Wheel? Standards, Interoperability and Digital Cultural Content; Preservation Risk Management for Web Resources: Virtual Remote Control in Cornell's Project Prism; Safekeeping: A Cooperative Approach to Building a Digital Preservation Resource; Object Persistence and Availability in Digital Libraries; Illinois Digital Cultural Heritage Community-Collaborative Interactions among Libraries, Museums and Elementary Schools.

    Science.gov (United States)

    Arms, William Y.; Hillmann, Diane; Lagoze, Carl; Krafft, Dean; Marisa, Richard; Saylor, John; Terizzi, Carol; Van de Sompel, Herbert; Gill, Tony; Miller, Paul; Kenney, Anne R.; McGovern, Nancy Y.; Botticelli, Peter; Entlich, Richard; Payette, Sandra; Berthon, Hilary; Thomas, Susan; Webb, Colin; Nelson, Michael L.; Allen, B. Danette; Bennett, Nuala A.; Sandore, Beth; Pianfetti, Evangeline S.

    2002-01-01

    Discusses digital libraries, including interoperability, metadata, and international standards; Web resource preservation efforts at Cornell University; digital preservation at the National Library of Australia; object persistence and availability; collaboration among libraries, museums and elementary schools; Asian digital libraries; and a Web…

  20. [The Lancisiana library as a resource for the transmission of medical culture].

    Science.gov (United States)

    Conforti, Maria; Fiorilla, Marco

    2002-01-01

    The Lancisiana Library was founded in the years 1711-1714 by Giovanni Maria Lancisi. Housed in the S. Spirito Hospital in Rome, its very structure and organization mirrors the scientific and medical culture of its founder. Lancisi meant to offer to the young apprentice, either physician or surgeon, the means foa a rational medical education, based on "plenty of patients" and a good choice of books. The comparison between the Library and some of Lancisi's works show his ideas about the relationship between the sciences - chemistry, natural history, mathematics and mechanics - and medicine, as well as the close relationship between medicine and surgery.

  1. Electronic Resources and Mission Creep: Reorganizing the Library for the Twenty-First Century

    Science.gov (United States)

    Stachokas, George

    2009-01-01

    The position of electronic resources librarian was created to serve as a specialist in the negotiation of license agreements for electronic resources, but mission creep has added more functions to the routine work of electronic resources such as cataloging, gathering information for collection development, and technical support. As electronic…

  2. A hybrid BAC physical map of potato: a framework for sequencing a heterozygous genome

    Directory of Open Access Journals (Sweden)

    de Boer Jan M

    2011-12-01

    Full Text Available Abstract Background Potato is the world's third most important food crop, yet cultivar improvement and genomic research in general remain difficult because of the heterozygous and tetraploid nature of its genome. The development of physical map resources that can facilitate genomic analyses in potato has so far been very limited. Here we present the methods of construction and the general statistics of the first two genome-wide BAC physical maps of potato, which were made from the heterozygous diploid clone RH89-039-16 (RH. Results First, a gel electrophoresis-based physical map was made by AFLP fingerprinting of 64478 BAC clones, which were aligned into 4150 contigs with an estimated total length of 1361 Mb. Screening of BAC pools, followed by the KeyMaps in silico anchoring procedure, identified 1725 AFLP markers in the physical map, and 1252 BAC contigs were anchored the ultradense potato genetic map. A second, sequence-tag-based physical map was constructed from 65919 whole genome profiling (WGP BAC fingerprints and these were aligned into 3601 BAC contigs spanning 1396 Mb. The 39733 BAC clones that overlap between both physical maps provided anchors to 1127 contigs in the WGP physical map, and reduced the number of contigs to around 2800 in each map separately. Both physical maps were 1.64 times longer than the 850 Mb potato genome. Genome heterozygosity and incomplete merging of BAC contigs are two factors that can explain this map inflation. The contig information of both physical maps was united in a single table that describes hybrid potato physical map. Conclusions The AFLP physical map has already been used by the Potato Genome Sequencing Consortium for sequencing 10% of the heterozygous genome of clone RH on a BAC-by-BAC basis. By layering a new WGP physical map on top of the AFLP physical map, a genetically anchored genome-wide framework of 322434 sequence tags has been created. This reference framework can be used for anchoring and

  3. Defining an open access resource strategy for research libraries:Part Ⅲ—The strategies and practices of National Science Library

    Institute of Scientific and Technical Information of China (English)

    Xiaolin; ZHANG; Xiwen; LIU; Lin; LI; Yan; ZENG; Li-Ping; KU

    2012-01-01

    Purpose:This paper describes the strategies and practices of National Science Library(NSL),Chinese Academy of Sciences(CAS)in promoting open access(OA)and developing OA resources.Design/methodology/approach:Multi-facet frameworks are devised to guide the development of OA strategies and practices.Key OA initiatives are briefly described along the main aspects of the strategies as they contribute to implementation of the OA strategies.Findings:NSL defined its role as the Chief OA Officer for CAS and a key OA promoter for China.Accordingly,NSL has engaged in multiple fronts of promoting OA,including development of OA strategies for CAS,establishment of itself as an OA knowledge&promotion center,development of the CAS IR system,and support for OA publishing by CAS authors.Research limitations:OA is still evolving,so are the strategies and practices as many actions are experimental and explorative in nature.Open books,open data,and open educational resources are yet to be covered.Comparative studies are needed,so is the evidence-based impact analysis.Practical implications:Institutions can adopt,adapt,or compare with examples and lessons learned are described here.Originality/value:The multi-faceted frameworks,working principles,and lessons learned are based on NSL’s practices which can be valuable to the overall OA development.

  4. Mass spectral reference libraries: an ever-expanding resource for chemical identification.

    Science.gov (United States)

    Stein, Stephen

    2012-09-04

    The basic principles, practices, and pitfalls in the process of compound identification by searching mass spectral reference libraries are presented. Factors affecting the identification process are discussed as members of one of three major contributors to identification confidence: prior probability, risk of false negative results, and risk of false positive results. More general concerns and the problem of "unknown unknowns" are then explored.

  5. 100 Colleges Sign Up with Google to Speed Access to Library Resources

    Science.gov (United States)

    Young, Jeffrey R.

    2005-01-01

    More than 100 colleges and universities have arranged to give people using the Google Scholar search engine on their campuses more-direct access to library materials. Google Scholar is a free tool that searches scholarly materials on the Web and in academic databases. The new arrangements essentially let Google know which online databases the…

  6. Reading Resources and Project LEER. Libraries and the Spanish-Speaking

    Science.gov (United States)

    Shepard, Marietta Daniels

    1970-01-01

    A discussion of the problems U.S. librarians and educators face in dealing with Spanish-speaking children and adults; and a description of Project LEER, a program to aid school and public libraries in the selection, procurement, and processing of books in Spanish. (JB)

  7. Print and Electronic Resources: Usage Statistics at Guru Gobind Singh Indraprastha University Library

    Science.gov (United States)

    Kapoor, Kanta

    2010-01-01

    Purpose: The purpose of this paper is to quantify the use of electronic journals in comparison with the print collections in the Guru Gobind Singh Indraprastha University Library. Design/methodology/approach: A detailed analysis was made of the use of lending services, the Xerox facility and usage of electronic journals such as Science Direct,…

  8. Environmental and Occupational Health Information Resources from the National Library of Medicine

    Institute of Scientific and Technical Information of China (English)

    Philip Wexler

    2004-01-01

    @@ For nearly forty years,the National Library of Medicine' s(NLM)Toxicology and Environmental Health Information Program(TEHIP) has been a signifcant leader in organizing and providing public access to an extensive storehouse of environmental, occupational medicine, and toxicological information through its online databases.

  9. Characteristic Resources Construction of Digital Library Resources Sharing:Taking Library of Ningxia as an Example%浅谈数字化图书馆资源共享中的特色资源建设--以宁夏图书馆为例

    Institute of Scientific and Technical Information of China (English)

    张娟

    2013-01-01

    在数字化图书馆资源共享工作的推进中,特色资源建设尤为重要。本文以宁夏图书馆为例,阐述如何建设图书馆的数字化特色资源。%In the promotion of digital library resource sharing, characteristic resource construction is particularly important. Based on the library of Ningxia as an example, this paper expounds how to construct library's digital resources.

  10. Electronic Information Resources (EIR Adoption in Private University Libraries: The Moderating Effect of Productivity and Relative Advantage on Perceived Usefulness

    Directory of Open Access Journals (Sweden)

    Izuagbe, Roland

    2016-03-01

    Full Text Available The study tested a hybrid model with constructs drawn from the Technology Acceptance Model (TAM and Diffusion of Innovation (DOI theory in order to examine the moderating effect of productivity and relative advantage (RA on perceived usefulness (PU vis-à-vis electronic information resources (EIR adoption in private university libraries in Ogun and Osun States of Nigeria. The descriptive research design was adopted in the study. The population consisted of 61 (55.0% librarians and 50 (45.0% library officers (totaling 116—100% in Babcock University, Bells University, Covenant University, Bowen University, Oduduwa University, and Redeemer's University. Purposive sampling procedure was adopted after which total enumeration was used since the total population is small. The questionnaire was used for data collection. Of the 116 copies of the questionnaire administered, 111 (95.7% were found usable. The instrument was structured based on a 4-point Likert agreement scale of Strongly Agree, Agree, Disagree, and Strongly Disagree. Data were analyzed using descriptive statistics like tables of frequency counts and percentage. The findings revealed that productivity and relative advantage are significant moderators of perceived usefulness of EIR adoption in private university libraries in Ogun and Osun States, Nigeria.

  11. On University Library Resource Sharing in University City%论大学城高校图书馆资源共享

    Institute of Scientific and Technical Information of China (English)

    钟筱莺

    2013-01-01

    大学城高校图书馆的资源共享存在一定的问题,而资源共享是时代发展的必然要求,可通过大学城不同高校图书馆之间资源共享来实现,以扩大不同高校图书馆的读者群,节约办学成本,提高高校办学效率.%In this paper, the present situation of university library resource sharing in university city is discussed. Resource sharing is an inevitable development of the times. It is to be realization by resourcing sharing among different university libraries in the university city and it can expand the readership of different university libraries. Furthermore, resource sharing can reduce the teaching cost and improve teaching efficiency.

  12. 图书馆电子信息资源利用效果评价研究%Study on Utilization Effect Evaluation of Library Electronic Information Resources

    Institute of Scientific and Technical Information of China (English)

    晋晓强; 贺培风; 何忠印

    2012-01-01

    This paper discusses utilization effect of electronic information resources combining with the characteristics of electronic information resources, makes evaluation system of library electronic information resource utilization effect from three aspects : the library, the users and the data providers, hoping for providing valuable reference for the efficient use of library electronic resources.%本文结合电子信息资源的特性对电子信息资源的利用进行初步探讨,从图书馆、用户和数据服务商三个角度构建图书馆电子信息资源利用效果的评价体系,希望能为图书馆电子信息资源的高效利用提供参考。

  13. 高校图书馆的人才资源准备%Preparation for personnel resources of academic libraries

    Institute of Scientific and Technical Information of China (English)

    苏俊彦

    2011-01-01

    文章分析了高校图书馆人才资源的优势以及影响高校图书馆人才发展的不利因素,提出了在人员老龄化、大学生毕业分配就业制度改革下高校图书馆人才资源准备的措施。%This paper analyzes the advantage of academic libraries' personnel resources and the disadvantages of influencing its development, and suggests solutions to preparing for persormel resources for academic libraries under the aging condition and the employment system reform of allocation of the graduates.

  14. Conditionally amplifiable BACs: switching from single-copy to high-copy vectors and genomic clones.

    Science.gov (United States)

    Wild, Jadwiga; Hradecna, Zdenka; Szybalski, Waclaw

    2002-09-01

    The widely used, very-low-copy BAC (bacterial artificial chromosome) vectors are the mainstay of present genomic research. The principal advantage of BACs is the high stability of inserted clones, but an important disadvantage is the low yield of DNA, both for vectors alone and when carrying genomic inserts. We describe here a novel class of single-copy/high-copy (SC/HC) pBAC/oriV vectors that retain all the advantages of low-copy BAC vectors, but are endowed with a conditional and tightly controlled oriV/TrfA amplification system that allows: (1) a yield of ~100 copies of the vector per host cell when conditionally induced with L-arabinose, and (2) analogous DNA amplification (only upon induction and with copy number depending on the insert size) of pBAC/oriV clones carrying >100-kb inserts. Amplifiable clones and libraries facilitate high-throughput DNA sequencing and other applications requiring HC plasmid DNA. To turn on DNA amplification, which is driven by the oriV origin of replication, we used copy-up mutations in the gene trfA whose expression was very tightly controlled by the araC-P(araBAD) promoter/regulator system. This system is inducible by L-arabinose, and could be further regulated by glucose and fucose. Amplification of DNA upon induction with L-arabinose and its modulation by glucose are robust and reliable. Furthermore, we discovered that addition of 0.2% D-glucose to the growth medium helped toward the objective of obtaining a real SC state for all BAC systems, thus enhancing the stability of their maintenance, which became equivalent to cloning into the host chromosome

  15. Construction of the Bac-to-Bac System of Bombyx mori Nucleopolyhedroviru

    Institute of Scientific and Technical Information of China (English)

    Jin-shan HUANG; Bi-fang HAO; Xiu-lian SUN; Fei DENG; Hua-lin WANG; Zhi-hong HU

    2007-01-01

    To construct the Bac-to-Bac expression system of Bombyx mori nucleopolyhedrovirus (BmNPV), a transfer vector was constructed which contained an Escherichia coli (E. coli) mini-F replicon and a lacZ: attTN7: lacZ cassette within the upstream and downstream regions of the BmNPV polyhedrin gene. B. mori larvae were cotransfected with wild-type BmNPV genomic DNA and the transfer vector through subcutaneous injection to generate recombinant viruses by homologous recombination in vivo. The genomic DNA of budded viruses extracted from the hemolymph of the transfected larvae was used to transform E. coli DH10B. Recombinant bacmids were screened by kanamycin resistance, PCR and restriction enzyme (REN) digestion. One of the bacmid colonies, BmBacJS13, which had similar REN profiles to that of wild-type BmNPV, was selected for further research. To investigate the infectivity of BmBacJS13, the polyhedrin gene was introduced into the bacmid and the resultant recombinant (BmBacJS13-ph) was transfected to BmN cells. The budded viruses were collected from the supernatant of the transfected cells and used for infecting BmN cells. Growth curve analysis indicated that BmBacJS13-ph had a similar growth curve to that of wild-type BmNPV. Bio-assays indicated that BmBacJS13-ph was also infectious to B. mori larvae.

  16. Network Based Educational Environment How Libraries and Librarians Become Organizers of Knowledge Access and Resources

    CERN Document Server

    Pettenati, M C; Pettenati, Corrado

    2000-01-01

    In this paper we will highlight some important issues which will influence the redefinition of roles and duties of libraries and librarians in a networked based educational environment. Although librarians will also keep their traditional roles of faculty support services as well as reference service and research assistance, we identify the participation in the instructional design process, the support in the evaluation, development and use of a proper authoring system and the customization of information access, as being the domains where libraries and librarians should mainly involve themselves in the next future and make profit of their expertise in information and knowledge organization in order to properly and effectively support the institutions in the use of Information Technology in education.

  17. Research on Human Resource Status of Hospital Libraries in Shandong Province%山东省医院图书馆人力资源现状研究

    Institute of Scientific and Technical Information of China (English)

    王今民; 沈小尉; 孙燕

    2009-01-01

    人力资源是医院图书馆赖以生存并可持续发展的主要因素.分析山东省医院图书馆人力资源现状,找出影响医院图书馆人力资源建设的因素,提出医院图书馆应隶属信息科管理、图书馆领导要树立以人为本的人力资源建设理念、确立本馆人力资源建设发展的定位与方向.%Human resources are the principle factor for survival and sustainable development of hospital libraries, the paper analyzes the status of human resources of hospital libraries in Shandong province where it could reveal the factors that effect human resources construction in hospital libraries, and then puts forward that hospital libraries should be under the management of information department,the leader of library should establish people-oriented concept for human resources construction and the developing trends of human resources construction should be established.

  18. Collaboration in electronic resource provision in university libraries: SHEDL, a Scottish case study

    OpenAIRE

    Kidd, T

    2009-01-01

    This case study examines the growth of collaboration among Scottish higher education institutions. Following a summary of the work of the Scottish Confederation of University and Research Libraries (SCURL), more detailed information is provided on collaboration in the fields of acquisition, licensing, selection, and purchasing. Some of the UK background is outlined, relating to NESLi2 in particular, in order to illuminate the options within Scotland. The origins of negotiations on electronic ...

  19. Agricultural libraries and information centres in China: cooperation, resource-sharing and networking

    OpenAIRE

    1990-01-01

    This research is aimed towards the design and planning of an agricultural library and information network in China. Systems approach is applied to the construction of descriptive, mathematical and hybrid (logical) models in the design phase of the system development cycle. Four major conventional network functions are chosen as core functions to be designed in detail. A number of procedures and issues are identified for both overall network and individual function design. The embodiment of th...

  20. Counting on COUNTER: The Current State of E-Resource Usage Data in Libraries

    Science.gov (United States)

    Welker, Josh

    2012-01-01

    Any librarian who has managed electronic resources has experienced the--for want of words--"joy" of gathering and analyzing usage statistics. Such statistics are important for evaluating the effectiveness of resources and for making important budgeting decisions. Unfortunately, the data are usually tedious to collect, inconsistently organized, of…

  1. As Blood Alcohol Content (BAC) Increases, So Does Impairment

    Science.gov (United States)

    ... on. Feature: Rethinking Drinking As Blood Alcohol Content (BAC) Increases, So Does Impairment Past Issues / Spring 2014 ... For purposes of law enforcement, blood alcohol content (BAC) is used to define intoxication and provides a ...

  2. Comparative physical maps derived from BAC end sequences of tilapia (Oreochromis niloticus

    Directory of Open Access Journals (Sweden)

    Lindblad-Toh Kerstin

    2010-11-01

    Full Text Available Abstract Background The Nile tilapia is the second most important fish in aquaculture. It is an excellent laboratory model, and is closely related to the African lake cichlids famous for their rapid rates of speciation. A suite of genomic resources has been developed for this species, including genetic maps and ESTs. Here we analyze BAC end-sequences to develop comparative physical maps, and estimate the number of genome rearrangements, between tilapia and other model fish species. Results We obtained sequence from one or both ends of 106,259 tilapia BACs. BLAST analysis against the genome assemblies of stickleback, medaka and pufferfish allowed identification of homologies for approximately 25,000 BACs for each species. We calculate that rearrangement breakpoints between tilapia and these species occur about every 3 Mb across the genome. Analysis of 35,000 clones previously assembled into contigs by restriction fingerprints allowed identification of longer-range syntenies. Conclusions Our data suggest that chromosomal evolution in recent teleosts is dominated by alternate loss of gene duplicates, and by intra-chromosomal rearrangements (~one per million years. These physical maps are a useful resource for comparative positional cloning of traits in cichlid fishes. The paired BAC end sequences from these clones will be an important resource for scaffolding forthcoming shotgun sequence assemblies of the tilapia genome.

  3. Discussion on the Resources Integration after Normal University Library Amalgamation%师范院校图书馆合并后资源整合探讨

    Institute of Scientific and Technical Information of China (English)

    李爽

    2012-01-01

    本文从高校图书馆信息管理系统的合并、藏书的剔旧工作、建立并完善“学科馆员”制度等方面,对师范院校图书馆合并后资源整合进行了探讨。%In this paper, the resources integration after normal university library amalgamation is discussed from the following three aspects: the amalgamation of university library information management system, the weeding of library collection, as well as the establishment and perfection of subject librarian system.

  4. Creating a Library of Climate Change Education Resources for Audiences in the Southeast United States

    Science.gov (United States)

    Carroll, J.; McNeal, K. S.; Williams, C. C.; Paz, J. O.; Cho, H. "; Nair, U. S.; Geroux, J.; Guthrie, C.; Wright, K.; Hill, J.

    2011-12-01

    The Climate Literacy Partnership in the Southeast (CLiPSE) is a part of the Climate Change Education Program supported by the National Science Foundation (http://CLiPSE-project.org). The established CLiPSE partnership is dedicated to improving climate literacy in the southeast and promoting scientifically accurate, formal educational resources for the K-12 classroom audience, as well as informal educational resources for audiences such as agriculture, education, leisure, and religious organizations, to name a few. The CLiPSE project has been successful in creating partnerships with the National Geographic Alliances, Departments of Education, and Mississippi Environmental Education Alliance, among others, to determine an effective strategic plan for reaching K-12 audiences. One goal in the strategic plan is to create a catalog of climate change education resources that are aligned to state standards in the SE. Eighty-seven resources from the Climate Literacy and Energy Awareness Network (http://cleanet.org) have been aligned with the state education standards for grades six through twelve in the southeast, beginning with science in Mississippi and expanding to include science and math in the remaining SE states. The criteria for aligning the existing resources includes: matching key terms, topics, and lesson activities with the content strands and essential skills included in the state science framework. By developing a searchable database containing climate resources already aligned with state standards, CLiPSE will have made these resources more appealing to educators in the SE, increasing the likelihood of resources being implemented in the classroom. The CLiPSE Climate Science Team has also created an inventory of scientifically sound, informal resources, which will be available for dispersion to appropriate audiences and communities. Cataloged resources, both formal and informal, grouped by a variety of means, to include audience, grade level, and resource

  5. 艺术院校教学资源库建设策略研究%Strategy Research on Teaching Resource Library Construction in Art Colleges

    Institute of Scientific and Technical Information of China (English)

    夏军

    2015-01-01

    The article expounds the significance of constructing teaching resource library combining the feature of art college. In this article, the writer proposes some tactics about the construction of teaching resource library, including constructing content, constructing ways, organization and manage, etc. The article would perhaps provide some references for the construction of teaching resource library in art colleges.%结合艺术院校特点,阐述建设教学资源库的意义;对艺术院校教学资源库的建设提出几点策略,就建设内容、建设方式、组织与管理等问题进行研究探讨,以期为艺术院校教学资源库的建设提供参考.

  6. Cloud Storage Mode of University Library Digital Resources%高校图书馆数字资源的云存储模式探讨

    Institute of Scientific and Technical Information of China (English)

    付晓红; 莫筱梅

    2012-01-01

    分析了传统存储模式下高校图书馆数字资源存储存在的问题,结合云存储的优势,讨论了应用云存储模式存储高校图书馆数字资源,提出并分析了两种具体的高校图书馆数字资源云存储模式。%This paper analyzes the problems of the storage of university libraries digital resources under traditional storage mode,discusses the storage of university libraries digital resources which apply cloud storage mode based on the advantage of cloud storage,proposes and analyzes two specific cloud storage modes of university libraries digital resource.

  7. Immunotoxin BAC5-CT treated nasopharyngeal carcinoma

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    In this study, immunotoxin (IT) was prepared by conjugating BAC5 and CT with SPDP. The effects of IT on NPC and its mechanisms were explored using double labeled with radioactive nuclides, immunography and electron microscope technique in vivo and in vitro. The specific concentration of BAC5 in the tumor area showed. The radioactivity rate of tumor/nontumor (T/NT) was up to 10.26. IT had cytotoxic effects both on the cultured CNE-2 cell line and tumor multicell spheroides. In vivo, the preliminary result indicated that IT also had a inhibitory action on the nude mice models bearing human NPC (Reported in another article). Under electron microscope, the necrosis and apoptosis of tumor cells were found. The membranes of most tumor cells were found intacted not or corrosined, some of them had the character of apoptosis, including reduce of tumor cells membrane villi, condensation of cytoplasm and pyknosis or cleavage of nuclear. There were many of apoptosis bodies, which were occasionally phagocytosed by tumor cells. The infiltration of immunocytoes in tumor tissue could be seen. The results indicated that BAC5 can specifically combine with NPC cells and BAC5-CT has the inhibitory effect on NPC in vitro and in vivo, mechanism of which may be related to the effects that ‘warhead' CT dissolute the membrane of tumor cells directly, or/and IT promote the infiltration of immunocytoes so as to induce the apoptosis of tumor cell.

  8. The Statistical Analysis on Digital Library Resource Usage -Taking Shanxi University Library as An Example%数字资源使用统计分析——以山西大学图书馆为例

    Institute of Scientific and Technical Information of China (English)

    段惠静

    2012-01-01

    Along with the universities' increasing investment to the library, the reasonable use of library resource becomes more and more important under the digital environment. At the same time, the investment of library digitalized collection' s proportion is getting larger and larger with the network development and the changing methods of scientific research. Thus, the assessing of digital library resource usage is the reflection of the library service a chievements. The article, by taking Shanxi University library as an example, systematically analyzes and explains the statistical data of resource usage related to digital service achievements so as to construct a relatively reasonable digital collection system by fulfilling the requirements of Shanxi University library.%随着高校对图书馆经费投入的不断增加,数字环境下图书馆的合理使用变得越来越重要。而图书馆数字化馆藏的投入也随着网络的发展和人们科研习惯的变迁所占的比重越来越大,因而,对数字资源的使用统计就成为反映数字图书馆服务绩效的重要指标。文章以山西大学图书馆为例,对于数字服务绩效评估相关的使用统计数据进行系统的分析与阐释,借以构建符合山西大学图书馆要求的相对合理的数字馆藏体系。

  9. A resource for CERN community: the Bookshop in the Central Library

    CERN Multimedia

    2008-01-01

    If you are looking for technical and scientific books on topics such as IT, Radiation Protection, Detectors and Experimental Techniques, Project Management, or simply for a good read, why not check whether the CERN Bookshop has it in stock? You will be surprised by its coverage in IT (150 titles) and physics (300 titles). In total the Bookshop sells around 500 titles from around 15 publishing houses. However, the offer is not limited to these publishers – the Bookshop staff are ready to order any title that exists in print. In addition, we welcome suggestions from the user community for new titles to be added to the stock. The title list - which is integrated into the CERN library catalogue – is available from http://cdsweb.cern.ch/collection/CERN Bookshop This Bookshop is located in the Central Library, Building 52 1-052 and is open on weekdays from 8.30 a.m. to 5.30 p.m. It can be contacted by e-mail (bookshop@cern.ch). CERN us...

  10. Use and Cost of Electronic Resources in Central Library of Ferdowsi University Based on E-metrics

    Directory of Open Access Journals (Sweden)

    Mohammad Reza Davarpanah

    2012-07-01

    Full Text Available The purpose of this study was to investigate the usage of electronic journals in Ferdowsi University, Iran based on e-metrics. The paper also aimed to emphasize the analysis of cost-benefit and the correlation between the journal impact factors and the usage data. In this study experiences of Ferdowsi University library on licensing and usage of electronic resources was evaluated by providing a cost-benefit analysis based on the cost and usage statistics of electronic resources. Vendor-provided data were also compared with local usage data. The usage data were collected by tracking web-based access locally, and by collecting vender-provided usage data. The data sources were one-year of vendor-supplied e-resource usage data such as Ebsco, Elsevier, Proquest, Emerald, Oxford and Springer and local usage data collected from the Ferdowsi university web server. The study found that actual usage values differ for vendor-provided data and local usage data. Elsevier has got the highest usage degree in searches, sessions and downloads. Statistics also showed that a small number of journals satisfy significant amount of use while the majority of journals were used less frequent and some were never used at all. The users preferred the PDF rather than HTML format. The data in subject profile suggested that the provided e-resources were best suited to certain subjects. There was no correlation between IF and electronic journal use. Monitoring the usage of e-resources gained increasing importance for acquisition policy and budget decisions. The article provided information about local metrics for the six surveyed vendors/publishers, e.g. usage trends, requests per package, cost per use as related to the scientific specialty of the university.

  11. Survey on User Satisfaction of Electronic Resources in University Libraries%高校图书馆电子资源用户满意度调查

    Institute of Scientific and Technical Information of China (English)

    陈姚竹; 马东

    2011-01-01

    通过分析影响用户对图书馆电子资源利用的因素,以美国顾客满意度指数模型为基础,提出关于各个因素的测量指标,对高校图书馆电子资源用户满意度进行测量。%Through analyzing the influencing factors on utilizing library electronic resources, based on the model of American customer satisfaction index, the paper puts forward corresponding measurement indexes, and then measures the user satisfaction on electronic resources of university library

  12. Probe into Digital Library's Resources Co-construction and Sharing%数字图书馆资源共建共享的探讨

    Institute of Scientific and Technical Information of China (English)

    王雅睿

    2015-01-01

    This paper analyzes the advantages of digital library's resources sharing,and puts forward some strategies for promoting digital library's resources co-construction and sharing, which include the technology strategy, talent strategy, legal strategy and industrial layout strategy.%分析了数字图书馆资源共享的优势,提出了促进数字图书馆资源共建共享的策略,包括技术策略、人才策略、法律策略、产业布局策略.

  13. 试论图书馆电子资源与纸本资源的协调发展%The coordinated development of library electronic resources and paper resources

    Institute of Scientific and Technical Information of China (English)

    冯雷

    2012-01-01

      纸本资源和电子资源属于公共图书馆文献资源中两个非常重要的资源类型,纸本资源和电子资源的协调发展在图书馆的可持续发展过程中起着非常重要的作用。本文对如何实现图书馆电子资源和纸本资源之间的协调发展进行了探讨,希望能够为图书馆文献资源的管理提供一些参考。%  The paper and electronic resources are two very important types of literature resources of public libraries, the coordinated development of the paper and electronic resources play a very important role in the sustainable development process of the library. This article discusses how to realize the coordinated development of library electronic resources and paper resources, hope to provide some reference for library literature resource management.

  14. 纸质图书是图书馆的核心资源%Paper Books : Core Resources in Library

    Institute of Scientific and Technical Information of China (English)

    郑丽君

    2012-01-01

    The rise of the electronic books brings certain impact to the paper books,but the impact is far from subversive.Due to the paper books giving the readers the best reading,as well as the advantage of preservation,it is still the core resources of libraries.%电子图书的崛起给纸质图书带来一定的冲击,但这种冲击远没有达到颠覆性的强度。由于纸质图书带给读者最佳的阅读,纸质图书具有保存的优越性,因而纸质图书是图书馆的核心资源。

  15. Information Needs and Use of Library Resources by Special Needs Students in Selected Government Schools in Kaduna State and Federal Capital Territory, Abuja, Nigeria

    Directory of Open Access Journals (Sweden)

    Christiana Alami Atabor

    2015-12-01

    Full Text Available This study is on the information needs and use of library resources by special needs students in selected government schools in Kaduna State and Federal Capital Territory, Abuja, Nigeria. The survey technique was employed in the study. Five schools (i.e., Kaduna State Special Education School; Government Technical College, Malali, Kaduna; Alhudahuda College Zaria; Government Secondary School, Kwali and Government Secondary School, Kuje were purposefully selected out of a total of seven. Data were collected using a structured questionnaire for two respondent groups (i.e., special needs students and teachers/librarians. A total of 5 teachers/librarians and 345 special needs students were selected for the study. The data collected were analyzed using descriptive statistics. The findings of the study show that the special needs students have high needs for information on education, employment opportunities, health matter, and human rights. The major information resources in the libraries are books, magazines, newspapers, and few Braille materials. In general, special needs students have a moderate level of satisfaction with the resources in the libraries. The major issue identified by special needs students is insufficient information resources, especially in Braille. On the part of the teachers/librarians, inadequate funds for the acquisition of information resources and the employment of qualified librarians/teachers are found to be the major challenges faced by the libraries.

  16. Open and Editable: Exploring Library Engagement in Open Educational Resource Adoption, Adaptation and Authoring

    Science.gov (United States)

    Walz, Anita R.

    2015-01-01

    Open Educational Resources (OER) have saved students millions of dollars in textbook costs and greatly expanded access to a wide variety of educational materials for countless numbers of students and life-long learners. OER have also saved teachers time and effort by allowing them to reuse, modify, and build on materials developed by other…

  17. "You Just Type in What You Are Looking for": Undergraduates' Use of Library Resources vs. Wikipedia

    Science.gov (United States)

    Colon-Aguirre, Monica; Fleming-May, Rachel A.

    2012-01-01

    This study presents findings from one-on-one interviews with 21 undergraduate students at a large public research university in the southeastern United States. While the preliminary focus of the study was to be students' opinions about and use of Wikipedia as a resource for course-related research, many of the interviews evolved into discussion…

  18. The Digital Edition 2.0 and the Digital Library: Services, not Resources

    NARCIS (Netherlands)

    van Zundert, J.; Boot, P.

    2011-01-01

    The article sketches a number of likely developments in scholarly digital editions and other scholarly digital resources. They will be composites of independent and distributed components, containing multiple media, and subject to permanent change. These trends seriously increase the as yet unsolved

  19. Transposon-mediated BAC transgenesis in zebrafish and mice

    Directory of Open Access Journals (Sweden)

    Sumiyama Kenta

    2009-10-01

    Full Text Available Abstract Background Bacterial artificial chromosomes (BACs are among the most widely used tools for studies of gene regulation and function in model vertebrates, yet methods for predictable delivery of BAC transgenes to the genome are currently limited. This is because BAC transgenes are usually microinjected as naked DNA into fertilized eggs and are known to integrate as multi-copy concatamers in the genome. Although conventional methods for BAC transgenesis have been very fruitful, complementary methods for generating single copy BAC integrations would be desirable for many applications. Results We took advantage of the precise cut-and-paste behavior of a natural transposon, Tol2, to develop a new method for BAC transgenesis. In this new method, the minimal sequences of the Tol2 transposon were used to deliver precisely single copies of a ~70 kb BAC transgene to the zebrafish and mouse genomes. We mapped the BAC insertion sites in the genome by standard PCR methods and confirmed transposase-mediated integrations. Conclusion The Tol2 transposon has a surprisingly large cargo capacity that can be harnessed for BAC transgenesis. The precise delivery of single-copy BAC transgenes by Tol2 represents a useful complement to conventional BAC transgenesis, and could aid greatly in the production of transgenic fish and mice for genomics projects, especially those in which single-copy integrations are desired.

  20. User experiences of evidence-based online resources for health professionals: User testing of The Cochrane Library

    Directory of Open Access Journals (Sweden)

    Glenton Claire

    2008-07-01

    Full Text Available Abstract Background Evidence-based decision making relies on easy access to trustworthy research results. The Cochrane Library is a key source of evidence about the effect of interventions and aims to "promote the accessibility of systematic reviews to anyone wanting to make a decision about health care". We explored how health professionals found, used and experienced The Library, looking at facets of user experience including findability, usability, usefulness, credibility, desirability and value. Methods We carried out 32 one-hour usability tests on participants from Norway and the UK. Participants both browsed freely and attempted to perform individually tailored tasks while "thinking aloud". Sessions were recorded and viewed in real time by researchers. Transcriptions and videos were reviewed by one researcher and one designer. Findings reported here reflect issues receiving a high degree of saturation and that we judge to be critical to the user experience of evidence-based web sites, based on principles for usability heuristics, web guidelines and evidence-based practice. Results Participants had much difficulty locating both the site and its contents. Non-native English speakers were at an extra disadvantage when retrieving relevant documents despite high levels of English-language skills. Many participants displayed feelings of ineptitude, alienation and frustration. Some made serious mistakes in correctly distinguishing between different information types, for instance reviews, review protocols, and individual studies. Although most expressed a high regard for the site's credibility, some later displayed a mistrust of the independence of the information. Others were overconfident, thinking everything on The Cochrane Library site shared the same level of quality approval. Conclusion Paradoxically, The Cochrane Library, established to support easy access to research evidence, has its own problems of accessibility. Health professionals

  1. Research on Experiences of Foreign Library Resource Sharing Service Innovation%国外图书馆资源共享服务创新经验研究

    Institute of Scientific and Technical Information of China (English)

    余若琪

    2015-01-01

    介绍国外尤其是美国图书馆信息资源共享服务创新的概况。认为国外图书馆在创新资源发现与传递、共享工作准则、创新性馆际合作模式等方面积累许多案例和经验,值得我国图书馆借鉴。我国图书馆及图书馆员要通过学习国外经验以提升自身业务水平,也要通过参与国内及国际图书馆组织而努力开展创新性服务实践。%The paper introduces general situation of foreign library, especially American library information resources sharing ser-vice innovation. It thinks that cases and experiences of foreign library on innovation resource discovery and delivery, sharing working norm, innovative interlibrary cooperation model are worth learning by Chinese library. Chinese library and librarian should learn foreign related experience to promote their professional level, and participate in national and international library organization to actively devel-op innovative service practice.

  2. Recommendations for protecting National Library of Medicine Computing and Networking Resources

    Energy Technology Data Exchange (ETDEWEB)

    Feingold, R.

    1994-11-01

    Protecting Information Technology (IT) involves a number of interrelated factors. These include mission, available resources, technologies, existing policies and procedures, internal culture, contemporary threats, and strategic enterprise direction. In the face of this formidable list, a structured approach provides cost effective actions that allow the organization to manage its risks. We face fundamental challenges that will persist for at least the next several years. It is difficult if not impossible to precisely quantify risk. IT threats and vulnerabilities change rapidly and continually. Limited organizational resources combined with mission restraints-such as availability and connectivity requirements-will insure that most systems will not be absolutely secure (if such security were even possible). In short, there is no technical (or administrative) {open_quotes}silver bullet.{close_quotes} Protection is employing a stratified series of recommendations, matching protection levels against information sensitivities. Adaptive and flexible risk management is the key to effective protection of IT resources. The cost of the protection must be kept less than the expected loss, and one must take into account that an adversary will not expend more to attack a resource than the value of its compromise to that adversary. Notwithstanding the difficulty if not impossibility to precisely quantify risk, the aforementioned allows us to avoid the trap of choosing a course of action simply because {open_quotes}it`s safer{close_quotes} or ignoring an area because no one had explored its potential risk. Recommendations for protecting IT resources begins with discussing contemporary threats and vulnerabilities, and then procedures from general to specific preventive measures. From a risk management perspective, it is imperative to understand that today, the vast majority of threats are against UNIX hosts connected to the Internet.

  3. Libraries for users services in academic libraries

    CERN Document Server

    Alvite, Luisa

    2010-01-01

    This book reviews the quality and evolution of academic library services. It revises service trends offered by academic libraries and the challenge of enhancing traditional ones such as: catalogues, repositories and digital collections, learning resources centres, virtual reference services, information literacy and 2.0 tools.studies the role of the university library in the new educational environment of higher educationrethinks libraries in academic contextredefines roles for academic libraries

  4. Information Resources Construction of Digital Library Based on Cloud Computing%基于云计算的数字图书馆信息资源建设

    Institute of Scientific and Technical Information of China (English)

    欧裕美

    2014-01-01

    介绍了数字图书馆信息资源建设现状,阐述了云计算的海量信息存储技术,讨论了云计算给数字图书馆信息资源建设带来的变革,探讨了基于云计算的数字图书馆信息资源建设面临的问题。%This paper introduces the current status of information resources construction of digital library, expounds the massive information storage technology of coud computing, discusses the changes brought about by the cloud computing to digital library, and probes into some problems existing in information resources construction of digital library.

  5. 日本图书馆的动漫资源建设特点%Characteristics of Comic Resources Construction in Japanese Libraries

    Institute of Scientific and Technical Information of China (English)

    黄如花; 李英子

    2015-01-01

    文章就日本图书馆的动漫资源建设特点进行了分析,发现日本几乎所有的图书馆均收藏动漫资源,在全国拥有一定数量的漫画专业图书馆,图书馆积极争取社会援助与民间资源,但也存在这缺乏完善的资源收集标准、动漫资源长期保存等难题与问题。%The paper analyses the characteristics of comic resources construction of Japanese libraries. Most of Japanese libraries collect comic resources;There is a certain amount of manga libraries;Libraries try to get social assistance and public resources;There is no comprehensive resourcecollectionstandards;There are problems for long-term preservation of comic resources.

  6. 云存储模式下图书馆数字资源建设%Library Digital Resources Construction Based on Cloud Storage Mode

    Institute of Scientific and Technical Information of China (English)

    曾祥文

    2011-01-01

    随着图书馆数字资源总量的快速增加,传统的存储方式在面对海量数据存储的需求上,存在着容量和性能的瓶颈。云存储作为一种新型的存储服务模式,为图书馆的海量数字资源建设提供了一种新的选择。在分析图书馆数字资源建设需求、介绍云存储概念的基础上,对基于云存储模式下图书馆数字资源的建设进行了探讨。%With the rapid increase in quantity of the library digital resources,there is capacity and performance of bottleneck when the traditional way of storage in the face of mass storage requirements.As a new storage service mode,cloud storage provides a new choice for the amount of library digital resources construction.Based on the analysis of library digital resources construction demand and the introduction of the concept of cloud storage,this paper discussed the library digital resources construction based on the cloud storage mode.

  7. Discussion on the Construction of Teaching Resources Library in Higher Vocational English Teaching%高职英语教学资源库建设探讨

    Institute of Scientific and Technical Information of China (English)

    陈彬

    2014-01-01

    教学资源库包括文本资源库、视频资源库、软件资源库、移动通讯英语学习平台等。要改变当前高职生学习兴趣差、英语教学课时有限、自主学习能力弱等问题,应该建设高职英语教学资源库,以提高教师的高职英语教学水平。%Teaching resources library includes ext resources library, video database, software resource library, mobile English learning platforms, etc.In order to change the current conditions such as vocational college students' low interest to learning, limited English teaching classes and students' weak autonomous learning abilities, English teaching resources libraries in higher vocational colleges should be set up to improve the level of English Teaching.

  8. Scholars' Satisfaction with Digital Library Collection and Gaps in the Provision of Effective Information Resources and Services: A Pakistani Perspective

    Science.gov (United States)

    Khan, Amjid; Ahmed, Shamshad; Masrek, Mohamad Noorman

    2014-01-01

    The study aims to explore the researchers' satisfaction with digital library resources and services and how they improved the research culture in Pakistani universities. A descriptive survey method was employed to achieve objectives of this study. Using stratified random sampling, for this survey we selected 14 public sector universities of…

  9. Utilization of Information and Communication Technology (ICT) Resources and Job Effectiveness among Library Staff in the University of Calabar and Cross River University of Technology, Nigeria

    Science.gov (United States)

    Ntui, Aniebiet Inyang; Inyang, Comfort Linus

    2015-01-01

    This study investigated utilization of Information and Communication Technology (ICT) resources and job effectiveness among library staff in the University of Calabar and Cross River University of Technology, Nigeria. To achieve the purpose of this study, four hypotheses were formulated to guide the study. Ex-post facto research design was adopted…

  10. Title II Elementary and Secondary Education Act (Public Law 89-10) as Amended. Annual Report, Fiscal Year 1972, School Library Resources, Textbooks, and Other Instructional Materials.

    Science.gov (United States)

    Office of Education (DHEW), Washington, DC.

    Title II of the Elementary and Secondary Education Act (ESEA) of 1965 (Public Law 89-10) as amended, provides direct Federal assistance for the acquisition of school library resources, textbooks, and other instructional materials for the use of children and teachers in public and private elementary and secondary schools. Its purpose is to improve…

  11. Making Higher Education More Affordable, One Course Reading at a Time: Academic Libraries as Key Advocates for Open Access Textbooks and Educational Resources

    Science.gov (United States)

    Okamoto, Karen

    2013-01-01

    Open access textbooks (OATs) and educational resources (OERs) are being lauded as a viable alternative to costly print textbooks. Some academic libraries are joining the OER movement by creating guides to open repositories. Others are promoting OATs and OERs, reviewing them, and even helping to create them. This article analyzes how academic…

  12. Study on Information Resource Services in Libraries under Network Environment%网络环境下图书馆信息资源服务研究

    Institute of Scientific and Technical Information of China (English)

    周亮; 袁艳

    2016-01-01

    The development of Internet era facilitates the development of information resource services in libraries and meanwhile brings new challenges. Network environment gives time features to information services in libraries, such as individualization of customer needs, simplicity of operation systems, diversification of information services, and mobilization of user behaviors. Based on current information resource service mode, problems of information resource services in libraries were examined, and development strategies were proposed to promote information resource services in libraries, and then better services for the public were provided.%互联网时代的发展促进了图书馆信息资源服务的发展,同时也为其带来了新挑战,网络环境赋予图书馆信息资源服务的时代特征,如用户需求个性化、系统操作简洁化、信息服务多样化、用户行为移动化等。以当前图书馆信息资源服务模式为基点,考察网络环境下图书馆信息资源服务面临的困境,提出发展策略,促进图书馆信息资源服务,更好地为广大读者服务。

  13. The complexity of Rhipicephalus (Boophilus microplus genome characterised through detailed analysis of two BAC clones

    Directory of Open Access Journals (Sweden)

    Valle Manuel

    2011-07-01

    Full Text Available Abstract Background Rhipicephalus (Boophilus microplus (Rmi a major cattle ectoparasite and tick borne disease vector, impacts on animal welfare and industry productivity. In arthropod research there is an absence of a complete Chelicerate genome, which includes ticks, mites, spiders, scorpions and crustaceans. Model arthropod genomes such as Drosophila and Anopheles are too taxonomically distant for a reference in tick genomic sequence analysis. This study focuses on the de-novo assembly of two R. microplus BAC sequences from the understudied R microplus genome. Based on available R. microplus sequenced resources and comparative analysis, tick genomic structure and functional predictions identify complex gene structures and genomic targets expressed during tick-cattle interaction. Results In our BAC analyses we have assembled, using the correct positioning of BAC end sequences and transcript sequences, two challenging genomic regions. Cot DNA fractions compared to the BAC sequences confirmed a highly repetitive BAC sequence BM-012-E08 and a low repetitive BAC sequence BM-005-G14 which was gene rich and contained short interspersed elements (SINEs. Based directly on the BAC and Cot data comparisons, the genome wide frequency of the SINE Ruka element was estimated. Using a conservative approach to the assembly of the highly repetitive BM-012-E08, the sequence was de-convoluted into three repeat units, each unit containing an 18S, 5.8S and 28S ribosomal RNA (rRNA encoding gene sequence (rDNA, related internal transcribed spacer and complex intergenic region. In the low repetitive BM-005-G14, a novel gene complex was found between to 2 genes on the same strand. Nested in the second intron of a large 9 Kb papilin gene was a helicase gene. This helicase overlapped in two exonic regions with the papilin. Both these genes were shown expressed in different tick life stage important in ectoparasite interaction with the host. Tick specific sequence

  14. The Data Model Resource Book A Library of Universal Data Models for All Enterprises

    CERN Document Server

    Silverston, Len

    2008-01-01

    A quick and reliable way to build proven databases for core business functionsIndustry experts raved about The Data Model Resource Book when it was first published in March 1997 because it provided a simple, cost-effective way to design databases for core business functions. Len Silverston has now revised and updated the hugely successful First Edition, while adding a companion volume to take care of more specific requirements of different businesses. Each volume is accompanied by a CD-ROM, which is sold separately. Each CD-ROM provides powerful design templates discussed in the books in a rea

  15. Comparative analysis of catfish BAC end sequences with the zebrafish genome

    Directory of Open Access Journals (Sweden)

    Abernathy Jason

    2009-12-01

    Full Text Available Abstract Background Comparative mapping is a powerful tool to transfer genomic information from sequenced genomes to closely related species for which whole genome sequence data are not yet available. However, such an approach is still very limited in catfish, the most important aquaculture species in the United States. This project was initiated to generate additional BAC end sequences and demonstrate their applications in comparative mapping in catfish. Results We reported the generation of 43,000 BAC end sequences and their applications for comparative genome analysis in catfish. Using these and the additional 20,000 existing BAC end sequences as a resource along with linkage mapping and existing physical map, conserved syntenic regions were identified between the catfish and zebrafish genomes. A total of 10,943 catfish BAC end sequences (17.3% had significant BLAST hits to the zebrafish genome (cutoff value ≤ e-5, of which 3,221 were unique gene hits, providing a platform for comparative mapping based on locations of these genes in catfish and zebrafish. Genetic linkage mapping of microsatellites associated with contigs allowed identification of large conserved genomic segments and construction of super scaffolds. Conclusion BAC end sequences and their associated polymorphic markers are great resources for comparative genome analysis in catfish. Highly conserved chromosomal regions were identified to exist between catfish and zebrafish. However, it appears that the level of conservation at local genomic regions are high while a high level of chromosomal shuffling and rearrangements exist between catfish and zebrafish genomes. Orthologous regions established through comparative analysis should facilitate both structural and functional genome analysis in catfish.

  16. Sequencing of 15 622 gene-bearing BACs clarifies the gene-dense regions of the barley genome.

    Science.gov (United States)

    Muñoz-Amatriaín, María; Lonardi, Stefano; Luo, MingCheng; Madishetty, Kavitha; Svensson, Jan T; Moscou, Matthew J; Wanamaker, Steve; Jiang, Tao; Kleinhofs, Andris; Muehlbauer, Gary J; Wise, Roger P; Stein, Nils; Ma, Yaqin; Rodriguez, Edmundo; Kudrna, Dave; Bhat, Prasanna R; Chao, Shiaoman; Condamine, Pascal; Heinen, Shane; Resnik, Josh; Wing, Rod; Witt, Heather N; Alpert, Matthew; Beccuti, Marco; Bozdag, Serdar; Cordero, Francesca; Mirebrahim, Hamid; Ounit, Rachid; Wu, Yonghui; You, Frank; Zheng, Jie; Simková, Hana; Dolezel, Jaroslav; Grimwood, Jane; Schmutz, Jeremy; Duma, Denisa; Altschmied, Lothar; Blake, Tom; Bregitzer, Phil; Cooper, Laurel; Dilbirligi, Muharrem; Falk, Anders; Feiz, Leila; Graner, Andreas; Gustafson, Perry; Hayes, Patrick M; Lemaux, Peggy; Mammadov, Jafar; Close, Timothy J

    2015-10-01

    Barley (Hordeum vulgare L.) possesses a large and highly repetitive genome of 5.1 Gb that has hindered the development of a complete sequence. In 2012, the International Barley Sequencing Consortium released a resource integrating whole-genome shotgun sequences with a physical and genetic framework. However, because only 6278 bacterial artificial chromosome (BACs) in the physical map were sequenced, fine structure was limited. To gain access to the gene-containing portion of the barley genome at high resolution, we identified and sequenced 15 622 BACs representing the minimal tiling path of 72 052 physical-mapped gene-bearing BACs. This generated ~1.7 Gb of genomic sequence containing an estimated 2/3 of all Morex barley genes. Exploration of these sequenced BACs revealed that although distal ends of chromosomes contain most of the gene-enriched BACs and are characterized by high recombination rates, there are also gene-dense regions with suppressed recombination. We made use of published map-anchored sequence data from Aegilops tauschii to develop a synteny viewer between barley and the ancestor of the wheat D-genome. Except for some notable inversions, there is a high level of collinearity between the two species. The software HarvEST:Barley provides facile access to BAC sequences and their annotations, along with the barley-Ae. tauschii synteny viewer. These BAC sequences constitute a resource to improve the efficiency of marker development, map-based cloning, and comparative genomics in barley and related crops. Additional knowledge about regions of the barley genome that are gene-dense but low recombination is particularly relevant.

  17. Engineering Faculty Indicate High Levels of Awareness and Use of the Library but Tend to Consult Google and Google Scholar First for Research Resources

    Directory of Open Access Journals (Sweden)

    Elaine Sullo

    2016-09-01

    Full Text Available A Review of: Zhang, L. (2015. Use of library services by engineering faculty at Mississippi State University, a large land grant institution. Science & Technology Libraries, 34(3, 272-286. http://dx.doi.org/10.1080/0194262X.2015.1090941 Objective – To investigate the engineering faculty’s information-seeking behaviour, experiences, awareness, and use of the university library. Design – Web-based survey questionnaire. Setting – The main campus of a state university in the United States of America. Subjects – 119 faculty members within 8 engineering departments. Methods – An email invitation to participate in a 16-item electronic survey questionnaire, with questions related to library use, was sent in the spring of 2015 to 119 engineering faculty members. Faculty were given 24 days to complete the survey, and a reminder email was sent 10 days after the original survey invitation. Main Results – Thirty-eight faculty members responded to the survey, representing a response rate of 32%. Overall, faculty had a high level of use and awareness of both online and physical library resources and services, although their awareness of certain scholarly communication services, such as data archiving and copyright advisory, was significantly lower. Faculty tend to turn to Google and Google Scholar when searching for information rather than turning to library databases. Faculty do not use social media to keep up with library news and updates. The library website, as well as liaison librarians, were cited as the primary sources for this type of information. Conclusions – The researcher concludes that librarians need to do a better job of marketing library resources, such as discipline-specific databases, as well as other library search tools. Because faculty use web search engines as a significant source of information, the author proposes further research on this behaviour, and suggests more action to educate faculty on different search tools

  18. Designing of a Decision Support System (DSS for resource allocation with genetic algorithm approach (Case Study: Central Library of Tarbiat Modares University

    Directory of Open Access Journals (Sweden)

    Alireza Hasanzadeh

    2014-09-01

    Full Text Available The allocation of information is one of the main responsibilities of a manager. In case there is a limitation in the available resources, the issue of resources allocation is raised. Universities and post graduate centers have faced limitations in accessing resources such as budget, human resources, physical space, etc. This problem results in inappropriate use of the approved budget in buying and sharing the different types of information resources, lack of easy access to information resources by users, and users’ dissatisfaction. This paper is intended to see whether the model of genetic algorithm can be used in helping library heads of university to develop a support system for the proper allocation of resources. The data of the central library of a university as the main core of DSS through using genetic algorithm in MATLAB was used in order to come up with a better distribution of effective resources. Research methodology used in this paper was field study and survey. The findings indicated that genetic algorithm was successful in achieving this end.

  19. Better Collaborative Working is Likely to Increase Uptake of Library Resources in a University Setting. A review of: Hightower, Barbara, Carolyn Rawl, and Michelle Schutt. “Collaborations for Delivering the Library to Students Through WebCT.” Reference Services Review 35.4 (2007: 541-51.

    Directory of Open Access Journals (Sweden)

    John Loy

    2008-06-01

    Full Text Available Objectives – To ascertain the extent to which university faculty members are integrating library resources within the WebCT course management system / managed learning environment. Also, to identify the reasons why faculty members are not integrating library resources within WebCT, and to explore their willingness to do so in future. Design – Case study with survey questionnaire and selected interview follow up.Setting – Urban campus of Auburn University in Montgomery, Alabama, United States of America.Subjects – One hundred and two members of faculty received a questionnaire. Five follow-up interviews were conducted.Methods – One hundred and two members of faculty with WebCT-supported courses received a questionnaire. They were asked to indicate if they linked to library resources, and if so which specific ones (e.g. library homepage, online catalogue, etc.. If they did not link to library resources they were asked to indicate the reason why not, either by selecting one or more of five pre-selected reasons, or by detailing their own. Follow-up interviews were conducted with five respondents, two of whom (Education and Nursing linked to library resources, and three of whom (from Business, Education and Science did not. Server log reports were also examined to identify how many students were entering library resources from WebCT.Main Results – The survey response rate was 28% (29 individuals, and of these Nursing and Sciences were the highest users with 7 individuals in each faculty. Of the 29 respondents only 7 (24% currently linked to library resources. Nursing were the biggest group with 10 links (38% of the total, Science with 6 (23% and 5 each from Liberal Arts and Education (19%. The resources chosen to link to were• Article databases (6• Library homepage (5• Ask a Librarian e-mail service(3• Net library e-book collection (3Six other library resources scored lower and two were not linked to at all. While few respondents

  20. The Electronic, Eclectic Library.

    Science.gov (United States)

    Dowlin, Kenneth E.

    1980-01-01

    Utilization of telecommunications and computer technology to increase access to information is identified as a primary goal for public libraries. To further service in the future, libraries should consider database services, online access to library resources, online community conferencing, community databases, network resource sharing,…

  1. Prediction of Digital Resource Cost in the University Library%高校图书馆数字资源的成本预测

    Institute of Scientific and Technical Information of China (English)

    谭凯波

    2013-01-01

    The digital resource cost in the university library including purchase cost and use cost, and the cost prediction can provide a reference for the construction of digital resources in a university library. The Markov model and grey prediction model are combined to discuss a scientific prediction method about digital resource cost in the university.%高校图书馆数字资源成本主要包括购置成本与使用成本,将马尔可夫模型与灰色预测模型相结合,对成本进行科学预测,可以为高校图书馆数字资源建设提供决策参考。

  2. 图书馆数字资源长期保存策略初探%Library Digital Resources Long-term Preservation Strategy

    Institute of Scientific and Technical Information of China (English)

    宁锐

    2011-01-01

    随着网络技术发展,数字信息的迅速激增。数字资源的长期保存就成为了数字图书馆发展所要面临的主要问题。本文通过数字资源长期保存面临的主要问题和基本策略的分析,论述了图书馆数字资源长期保存的策略。%With the development of network technology,the rapid rise of digital information.Long term preservation of digital resources of digital library development becomes the major issues facing the.This article through the long-term preservation of digital resources and the major problems facing the basic strategy analysis,discusses the library digital resources long-term preservation strategy.

  3. Targeted isolation, sequence assembly and characterization of two white spruce (Picea glauca BAC clones for terpenoid synthase and cytochrome P450 genes involved in conifer defence reveal insights into a conifer genome

    Directory of Open Access Journals (Sweden)

    Ritland Carol

    2009-08-01

    Full Text Available Abstract Background Conifers are a large group of gymnosperm trees which are separated from the angiosperms by more than 300 million years of independent evolution. Conifer genomes are extremely large and contain considerable amounts of repetitive DNA. Currently, conifer sequence resources exist predominantly as expressed sequence tags (ESTs and full-length (FLcDNAs. There is no genome sequence available for a conifer or any other gymnosperm. Conifer defence-related genes often group into large families with closely related members. The goals of this study are to assess the feasibility of targeted isolation and sequence assembly of conifer BAC clones containing specific genes from two large gene families, and to characterize large segments of genomic DNA sequence for the first time from a conifer. Results We used a PCR-based approach to identify BAC clones for two target genes, a terpene synthase (3-carene synthase; 3CAR and a cytochrome P450 (CYP720B4 from a non-arrayed genomic BAC library of white spruce (Picea glauca. Shotgun genomic fragments isolated from the BAC clones were sequenced to a depth of 15.6- and 16.0-fold coverage, respectively. Assembly and manual curation yielded sequence scaffolds of 172 kbp (3CAR and 94 kbp (CYP720B4 long. Inspection of the genomic sequences revealed the intron-exon structures, the putative promoter regions and putative cis-regulatory elements of these genes. Sequences related to transposable elements (TEs, high complexity repeats and simple repeats were prevalent and comprised approximately 40% of the sequenced genomic DNA. An in silico simulation of the effect of sequencing depth on the quality of the sequence assembly provides direction for future efforts of conifer genome sequencing. Conclusion We report the first targeted cloning, sequencing, assembly, and annotation of large segments of genomic DNA from a conifer. We demonstrate that genomic BAC clones for individual members of multi-member gene

  4. 高校图书馆人力资源开发与管理探究%Study on Human Resources Development and Management in Academic Library --For library of Yangtze Normal University an e

    Institute of Scientific and Technical Information of China (English)

    刘晓莉

    2011-01-01

    Based on the state of human resources of Academic library, this article points out the problems of human resources management in libraries at present. Some suggestions, which includes librarian's inspection and training and the idea of human oriented, sho%文章就高校图书馆人力资源的现状进行分析,指出了存在的问题,提出了加强馆员的考核及培训和强化以人为本的理念是当前改善高校图书馆人力资源管理现状的重要举措。

  5. Resource Construction of College Library under the Background of Big Data%大数据背景下高校图书馆的资源建设

    Institute of Scientific and Technical Information of China (English)

    王晔煊; 杨艳君

    2013-01-01

    大数据时代,图书馆将在资源建设、读者服务、组织管理等方面面临着严峻的挑战。数字图书馆是图书馆信息化建设的重要组成部分。文章论述了大数据的内涵,分析了高校数字资源建设中存在的问题,进而对图书馆数字资源建设进行探讨。%Libraries will face severe challenges in terms of resource construction,reader service and organiza-tional management in an era of big data. Digital library is an important part of informatization of library. The paper discusses the connotation of big data,analyzes the problems facing digital resource construction of colle-ges and further explores the digital resource construction of library under background of big data.

  6. Fungicidal mechanisms of the antimicrobial peptide Bac8c.

    Science.gov (United States)

    Lee, Wonyoung; Lee, Dong Gun

    2015-02-01

    Bac8c (RIWVIWRR-NH2) is an analogue peptide derived through complete substitution analysis of the linear bovine host defense peptide variant Bac2A. In the present study, the antifungal mechanism of Bac8c against pathogenic fungi was investigated, with a particular focus on the effects of Bac8c on the cytoplasmic membrane. We used bis-(1,3-dibutylbarbituric acid) trimethine oxonol [DiBAC4(3)] staining and 3,3'-dipropylthiacarbocyanine iodide [DiSC3(5)] assays to show that Bac8c induced disturbances in the membrane potential of Candida albicans. An increase in membrane permeability and suppression of cell wall regeneration were also observed in Bac8c-treated C. albicans. We studied the effects of Bac8c treatment on model membranes to elucidate its antifungal mechanism. Using calcein and FITC-labeled dextran leakage assays from Bac8c-treated large unilamellar vesicles (LUVs) and giant unilamellar vesicles (GUVs), we found that Bac8c has a pore-forming action on fungal membranes, with an estimated pore radius of between 2.3 and 3.3 nm. A membrane-targeted mechanism of action was also supported by the observation of potassium release from the cytosol of Bac8c-treated C. albicans. These results indicate that Bac8c is considered as a potential candidate to develop a novel antimicrobial agent because of its low-cost production characteristics and high antimicrobial activity via its ability to induce membrane perturbations in fungi.

  7. Integrated library systems.

    Science.gov (United States)

    Goldstein, C M

    1983-07-01

    The development of integrated library systems is discussed. The four major discussion points are (1) initial efforts; (2) network resources; (3) minicomputer-based systems; and (4) beyond library automation. Four existing systems are cited as examples of current systems.

  8. 广州图书馆的家谱资源建设%Genealogy Resources Construction in Guangzhou Library

    Institute of Scientific and Technical Information of China (English)

    何映雯

    2014-01-01

    家谱与正史、方志是构成中华历史文献的三大支柱,它们从不同角度补充史实,使历史面貌、时代精神和社会风尚得以更真实、更全面的呈现,对历史学、人口学及社会学等学科的深入研究具有不可替代的独特功能。阐述了建设家谱资源的意义,探讨了广州图书馆在家谱资源的征集、整理、开发及服务方面所做的工作。%ABSTRACT:The genealogy, official history and chorography constitute the three pillars of Chinese historical documents, they supplement historical facts from different angles, making the historical appearance, time spirit and social custom get more real and more comprehensive presentation, and have an irreplaceable special function for the deep study of history, demography, sociology and other subjects. This paper expounds the significance of the construction of genealogy resources, and probes into the works that Guangzhou Library has done in aspects of genealogy resources’ collection, sorting, development, and service, etc.

  9. Study in Grey and White: Measuring the Impact of the 8Rs Canadian Library Human Resources Study

    Directory of Open Access Journals (Sweden)

    Allison Sivak

    2009-06-01

    Full Text Available Objective – To use the 8Rs Canadian Library Human Resources Study (the 8Rs Study as a test case to develop a model for assessing research impact in LIS. Methods – Three different methods of citation analysis which take into account the changing environment of scholarly communications. These include a ‚manual‛ method of locating citations to the 8Rs Study through a major LIS database, an enhanced-citation tool Google Scholar, and a general Google search to locate Study references in non-scholarly documents Results – The majority of references (82% were found using Google or Google Scholar; the remainder were located via LISA. Each method had strengths and limitations.Conclusion - In-depth citation analysis provides a promising method of understanding the reach of published research. This investigation’s findings suggest the need for improvements in LIS citation tools, as well as digital archiving practices to improve the accessibility of references for measuring research impact. The findings also suggest the merit of researchers and practitioners defining levels of research impact, which will assist researchers in the dissemination of their work.

  10. Modification of bacterial artificial chromosomes (BACs) and preparation of intact BAC DNA for generation of transgenic mice.

    Science.gov (United States)

    Gong, Shiaoching; Yang, X William

    2005-05-01

    BAC transgenesis is a powerful tool for the study of gene expression and gene function in the mouse in vivo. In this unit, detailed protocols are provided for modification (i.e., marker gene insertion, deletion, or point mutation) of BACs by homologous recombination in E. coli. This method utilizes a shuttle vector that allows transient expression of the E. coli RecA gene to support homologous recombination in the BAC host bacteria. In addition, two protocols are provided for purification of BAC DNA for microinjection to generate transgenic mice. Since BAC DNA is prone to degradation, which may introduce positional effects in transgenic mice, two methods are given for purification of intact BAC DNA for subsequent microinjection.

  11. Research on the Value Creation of Libraries Based on Information Resources%图书馆基于信息资源的价值创造研究

    Institute of Scientific and Technical Information of China (English)

    赵珊珊

    2011-01-01

    As the knowledge and information provided institutions, libraries confronts with how to optimize the utilization of information resources. There is a dynamic conversion process between information resources and value creation in libraries. In the value creation process, libraries should formulate strategic plan from the standpoint of strategic management process, adjust organization structure and implement management transform according to the staged change of external environment, plan and integrate resources, change the service mode, and realize the value creation based on information resources, so as to form the sustainable service advantage.%图书馆作为知识信息提供机构,面临着如何优化信息资源利用的问题。图书馆的信息资源从产生到价值创造之间存在一个动态的转化过程。图书馆在价值创造过程中,需要从战略管理流程角度进行战略规划,根据外部环境的阶梯式变化要求适时地进行组织结构调整和管理变革、规划和整合资源、转变服务模式,实现基于信息资源的价值创造,从而形成可持续的服务优势。

  12. 医学教学资源库构建与共享初探%Medical Teaching Resource Library Building and Sharing Study

    Institute of Scientific and Technical Information of China (English)

    吴双全

    2012-01-01

    This paper summarized the status of the medical library of teaching resources applications and advantages of the teaching of medical colleges and teaching resource library to be included in the basic content and teaching resources library,through personal experience and the construction of the repository of medical teaching experience,to identify the medical colleges and teaching resources inventory problems and solutions,I believe has a certain reference value.%本文通过对医学教学资源库的现状分析,概括医学院校教学资源库需包含的基本内容以及教学资源库再教学中的应用及优势,通过对医学教学资源库建设的切身经验和体会,找出医学院校教学资源库存在的问题及解决办法,相信具有一定的参考价值。

  13. A collection of books from Bielany in the Jagiellonian Library. Security, maintenance and availability of resources. A notice on the progress of work, June 2011

    Directory of Open Access Journals (Sweden)

    Jacek Partyka

    2011-12-01

    Full Text Available In the autumn of 2009 the Jagiellonian Library became the depositary of a large, valuable and interesting collection of printed books from the library of the Camaldolese Monastery in Bielany (Krakow. The work on processing the collection began almost immediately after all the prints had been taken over by the Jagiellonian Library. Two groups of employees have been involved in the work, both from the Resource Processing Department and the Antique Books Department, in line with the natural division into modern and old prints. The Camaldolese collection from Bielany has been enclosed in the monastery and unavailable to most readers; now the very books which for many years have been the source of knowledge, assistance and cognitive tools to a narrow circle of monks may be accessed by all interested persons. In the next year, after basic processing of the collection, the books will create an extremely interesting research opportunity.

  14. The Oryza map alignment project: Construction, alignment and analysis of 12 BAC fingerprint/end sequence framework physical maps that represent the 10 genome types of genus Oryza

    Science.gov (United States)

    The Oryza Map Alignment Project (OMAP) provides the first comprehensive experimental system for understanding the evolution, physiology and biochemistry of a full genus in plants or animals. We have constructed twelve deep-coverage BAC libraries that are representative of both diploid and tetraploid...

  15. A dense genetic linkage map for common carp and its integration with a BAC-based physical map.

    Directory of Open Access Journals (Sweden)

    Lan Zhao

    Full Text Available BACKGROUND: Common carp (Cyprinus carpio is one of the most important aquaculture species with an annual global production of 3.4 million metric tons. It is also an important ornamental species as well as an important model species for aquaculture research. To improve the economically important traits of this fish, a number of genomic resources and genetic tools have been developed, including several genetic maps and a bacterial artificial chromosome (BAC-based physical map. However, integrated genetic and physical maps are not available to study quantitative trait loci (QTL and assist with fine mapping, positional cloning and whole genome sequencing and assembly. The objective of this study was to integrate the currently available BAC-based physical and genetic maps. RESULTS: The genetic map was updated with 592 novel markers, including 312 BAC-anchored microsatellites and 130 SNP markers, and contained 1,209 genetic markers on 50 linkage groups, spanning 3,565.9 cM in the common carp genome. An integrated genetic and physical map of the common carp genome was then constructed, which was composed of 463 physical map contigs and 88 single BACs. Combined lengths of the contigs and single BACs covered a physical length of 498.75 Mb, or around 30% of the common carp genome. Comparative analysis between common carp and zebrafish genomes was performed based on the integrated map, providing more insights into the common carp specific whole genome duplication and segmental rearrangements in the genome. CONCLUSION: We integrated a BAC-based physical map to a genetic linkage map of common carp by anchoring BAC-associated genetic markers. The density of the genetic linkage map was significantly increased. The integrated map provides a tool for both genetic and genomic studies of common carp, which will help us to understand the genomic architecture of common carp and facilitate fine mapping and positional cloning of economically important traits for

  16. Generation of BAC transgenic epithelial organoids.

    Directory of Open Access Journals (Sweden)

    Gerald Schwank

    Full Text Available Under previously developed culture conditions, mouse and human intestinal epithelia can be cultured and expanded over long periods. These so-called organoids recapitulate the three-dimensional architecture of the gut epithelium, and consist of all major intestinal cell types. One key advantage of these ex vivo cultures is their accessibility to live imaging. So far the establishment of transgenic fluorescent reporter organoids has required the generation of transgenic mice, a laborious and time-consuming process, which cannot be extended to human cultures. Here we present a transfection protocol that enables the generation of recombinant mouse and human reporter organoids using BAC (bacterial artificial chromosome technology.

  17. Integration of Library Services in the System of Digital Libraries

    Science.gov (United States)

    Bezdushnyj, A. N.; Kalenov, N. E.; Kouriv, P. M.; Serebryakov, V. A.

    The article is devoted to the functional extension of the Integrated System of Information Resources of RAS (ISIR RAS - RFBR 99-07-90139 project). This extension (Library Subsystem) provides integration of library services in ISIR RAS electronic libraries system. Library Subsystem implements standard library services such as information about free and ordered copies of editions, creation of the library orders for edition etc. The Library Subsystem is an integrated solution; it's based on ISIR data model and technologies.

  18. A high-throughput strategy for screening of bacterial artificial chromosome libraries and anchoring of clones on a genetic map constructed with single nucleotide polymorphisms

    Directory of Open Access Journals (Sweden)

    Deal Karin R

    2009-01-01

    Full Text Available Abstract Background Current techniques of screening bacterial artificial chromosome (BAC libraries for molecular markers during the construction of physical maps are slow, laborious and often assign multiple BAC contigs to a single locus on a genetic map. These limitations are the principal impediment in the construction of physical maps of large eukaryotic genomes. It is hypothesized that this impediment can be overcome by screening multidimensional pools of BAC clones using the highly parallel Illumina GoldenGate™ assay. Results To test the efficacy of the Golden Gate assay in BAC library screening, multidimensional pools involving 302976 Aegilops tauschii BAC clones were genotyped for the presence/absence of specific gene sequences with multiplexed Illumina GoldenGate oligonucleotide assays previously used to place single nucleotide polymorphisms on an Ae. tauschii genetic map. Of 1384 allele-informative oligonucleotide assays, 87.6% successfully clustered BAC pools into those positive for a BAC clone harboring a specific gene locus and those negative for it. The location of the positive BAC clones within contigs assembled from 199190 fingerprinted Ae. tauschii BAC clones was used to evaluate the precision of anchoring of BAC clones and contigs on the Ae. tauschii genetic map. For 41 (95% assays, positive BAC clones were neighbors in single contigs. Those contigs could be unequivocally assigned to loci on the genetic map. For two (5% assays, positive clones were in two different contigs and the relationships of these contigs to loci on the Ae. tauschii genetic map were equivocal. Screening of BAC libraries with a simple five-dimensional BAC pooling strategy was evaluated and shown to allow direct detection of positive BAC clones without the need for manual deconvolution of BAC clone pools. Conclusion The highly parallel Illumina oligonucleotide assay is shown here to be an efficient tool for screening BAC libraries and a strategy for high

  19. Partial complementation of Sinorhizobium meliloti bacA mutant phenotypes by the Mycobacterium tuberculosis BacA protein.

    Science.gov (United States)

    Arnold, M F F; Haag, A F; Capewell, S; Boshoff, H I; James, E K; McDonald, R; Mair, I; Mitchell, A M; Kerscher, B; Mitchell, T J; Mergaert, P; Barry, C E; Scocchi, M; Zanda, M; Campopiano, D J; Ferguson, G P

    2013-01-01

    The Sinorhizobium meliloti BacA ABC transporter protein plays an important role in its nodulating symbiosis with the legume alfalfa (Medicago sativa). The Mycobacterium tuberculosis BacA homolog was found to be important for the maintenance of chronic murine infections, yet its in vivo function is unknown. In the legume plant as well as in the mammalian host, bacteria encounter host antimicrobial peptides (AMPs). We found that the M. tuberculosis BacA protein was able to partially complement the symbiotic defect of an S. meliloti BacA-deficient mutant on alfalfa plants and to protect this mutant in vitro from the antimicrobial activity of a synthetic legume peptide, NCR247, and a recombinant human β-defensin 2 (HBD2). This finding was also confirmed using an M. tuberculosis insertion mutant. Furthermore, M. tuberculosis BacA-mediated protection of the legume symbiont S. meliloti against legume defensins as well as HBD2 is dependent on its attached ATPase domain. In addition, we show that M. tuberculosis BacA mediates peptide uptake of the truncated bovine AMP, Bac7(1-16). This process required a functional ATPase domain. We therefore suggest that M. tuberculosis BacA is important for the transport of peptides across the cytoplasmic membrane and is part of a complete ABC transporter. Hence, BacA-mediated protection against host AMPs might be important for the maintenance of latent infections.

  20. High Expression of Insulin-like Growth Factor H (IGF-Ⅱ) Using Bac-to-Bac Expression System

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective In order to obtain mature insulin-like growth factor- Ⅱ ( IGF- Ⅱ ), we used Bac-to-Bac baculovirus expression system. Methods Firstly the IGF- Ⅱ cDNA was cloned into a donor plasmid pFastBac1 and the recombinant pFastBac1 was then introduced into competent cells DH 10Bac. Recombinant bacmids were constructed by transposing a mini-Tn7 element from a donor plasmid pFastBac1 to the mini-attTn7 attachment site on the bacmid where the Tn7 transposition functions were provided in trans by a helper plasmid, and then used to transfect Sf9 insect cells to get recombinant baculovirus. The recombinant baculovirus was used to infect insect cells. Results Agarose gel analysis showed that recombinant donor plasmid pFastBac1 was constructed successfully; Agarose gel analysis of PCR products confirmed recombinant bacmid ; SDS-PAGE and Western Blotting showed that a 7KD protein band appeared. Conclusion The mature IGF- Ⅱ with immunogenecity has been expressed and produced by using Bac-to-Bac expression system.

  1. Comparison of Approval Plan Profiles and Supplementary Collection Development Activities in Selected ARL Libraries. A Report to the Council on Library Resources.

    Science.gov (United States)

    Snoke, Helen Lloyd; Loup, Jean L.

    This study was conducted to learn the extent to which member institutions of the Association of Research Libraries (ARL) with Blackwell North America (B/NA) approval plans covering philosophy and political science are duplicating collections, and to determine what supplementary collection development practices are being used. Initial data were…

  2. 血液学课程 MICM资源库的建设与应用%Construction and application of MICM resource library in teaching of hematologic diseases

    Institute of Scientific and Technical Information of China (English)

    王真真; 左学兰; 吴三云; 刘莉; 沈辉; 陈飞

    2016-01-01

    目的:建立和应用血液学课程MICM资源库,提高教学效果。方法收集临床常见的各种血液病患者的骨髓和外周血,常规方法制片, Wright 染色后显微镜下观察形态学( morph-ology,M),流式细胞仪方法检测细胞免疫表型(immunology,I),G 带方法制作细胞遗传学核型(cytogenetics,C),分子生物学方法(molecular biology,M)检测融合基因及基因突变,再将这些标本和结果制作成血液学MICM电子资源数据库并应用于教学和实习。结果建立了专门用于教学的血液学资源库,通过在不同学习阶段的学生中应用,增加了学生学习的积极性和主动性,提高了血液学教学效果及质量。结论建立和应用血液学MICM资源库有利于提高血液学教学质量,并对内科学教学资源平台建设具有借鉴意义。%Objective To construct MICM ( morphology , immunology , cytogenetics , and molecular biology) resource library and improve teaching effectiveness of hematologic diseases . Methods Peripheral blood and bone marrow were obtained from patients with typical hematologic diseases .Cell morphology was determined with Wright stain , and flow cytometry was used to detect immunophenotype of the cells.Karyotype of bone marrow was analyzed by G-banding, and fusion gene transcript was detected by molecular biology technique .All slides , observed and scanned under the electron microscope together with electronic resource , were collected and categorized for MICM resource library .The MICM resource library was then used for teaching of hematologic diseases and clinical practice .Results MICM resource library was successfully constructed and used in the teaching of hematologic diseases , which increased students'learning enthusiasm and initiative , and improved the efficiency and quality of teaching . Conclusion Construction and application of MICM resource library can help improve teaching effectiv

  3. Electronic Library: A TERI Experiment.

    Science.gov (United States)

    Kar, Debal C.; Deb, Subrata; Kumar, Satish

    2003-01-01

    Discusses the development of Electronic Library at TERI (The Energy and Resources Institute, New Delhi). Highlights include: hardware and software used; the digital library/Virtual Electronic Library; directory of Internet journals; virtual reference resources; electronic collection/Physical Electronic Library; downloaded online full-length…

  4. Sports Education Library Information Services

    Institute of Scientific and Technical Information of China (English)

    许晓峰

    2014-01-01

    Library website, is the first window for readers to understand library information services. Sports Education academy library fully take advantage of homepage, combine open access resource searched with the library collections, after targeted collection, selection, sorting, processing, clustering or reorganization, establish a navigation system of open access resource of physical Sports Education.

  5. There is a Relationship between Resource Expenditures and Reference Transactions in Academic Libraries. A Review of: Dubnjakovic, A. (2012. Electronic resource expenditure and the decline in reference transaction statistics in academic libraries. Journal of Academic Librarianship, 38(2, 94-100. doi:10.1016/j.acalib.2012.01.001

    Directory of Open Access Journals (Sweden)

    Annie M. Hughes

    2013-03-01

    Full Text Available Objective – To provide an analysis of the impact of expenditures on electronic resourcesand gate counts on the increase or decrease in reference transactions.Design – Analysis of results of existing survey data from the National Center for Educational Statistics (NCES 2006 Academic Library Survey(ALS.Setting – Academic libraries in the United States.Subjects – 3925 academic library respondents.Methods – The author chose to use survey data collected from the 2006 ALS conducted bythe NCES. The survey included data on various topics related to academic libraries, but in the case of this study, the author chose to analyze three of the 193 variables included. The three variables: electronic books expenditure, computer hardware and software, and expenditures on bibliographic utilities, were combined into one variable called electronic resource expenditure. Gate counts were also considered as a variable. Electronic resource expenditure was also split as a variable into three groups: low, medium, and high. Multiple regression analysis and general linear modeling, along with tests of reliability, were employed. Main Results – The author determined that low, medium, and high spenders with regard to electronic resources exhibited differences in gate counts, and gate counts have an effect on reference transactions in any given week. Gate counts tend to not have much of an effect on reference transactions for the higher spenders, and higher spenders tend to have a higher number of reference transactions overall. Low spenders have lower gate counts and also a lower amount of reference transactions.Conclusion – The findings from this study show that academic libraries spending more on electronic resources also tend to have an increase with regard to reference transactions. The author also concludes that library spaces are no longer the determining factor with regard to number of reference transactions. Spending more on electronic resources is

  6. The Egyptian Web Logs as an Information Resource with a Special Indication to Library and Librarian's Blogs

    Directory of Open Access Journals (Sweden)

    Shayma' Ismael Abbas Ismael

    2007-06-01

    Full Text Available An article is discussing The Egyptian Web Logs ,library blogs and librarian's blogs at the world wide web as one of information electronic sources so this article is answering on some questions. what is conception of blogs?, What is historic development and origin of blogs?, What are reasons to fame and spread blogs between internet employees ?, what is blogging software to create blog?, what is directory and search engine of blogs?, what is characteristics of successful blogs? and concentrates on Egyptian weblogs, what is the most famous Egyptian blogs in the world wide web ? and refer to relationship between the Egyptian weblogs and policy. and special indication to blogs in library and information science which include library blogs and librarian's blogs. this article also is answering on some questions about library blogs and librarian's blogs. what is meaning of library blogs and librarian's blogs? what is purposes and aims of library blogs and librarian's blogs? what is directory of library blogs and librarian's blogs? Are there sorts of library blogs and librarian's blogs? and What are examples of library blogs and librarian's blogs available at the world wide?. to give overview about this electronic source at all its parts.

  7. Sequencing of a QTL-rich region of the Theobroma cacao genome using pooled BACs and the identification of trait specific candidate genes

    Directory of Open Access Journals (Sweden)

    Blackmon Barbara P

    2011-07-01

    Full Text Available Abstract Background BAC-based physical maps provide for sequencing across an entire genome or a selected sub-genomic region of biological interest. Such a region can be approached with next-generation whole-genome sequencing and assembly as if it were an independent small genome. Using the minimum tiling path as a guide, specific BAC clones representing the prioritized genomic interval are selected, pooled, and used to prepare a sequencing library. Results This pooled BAC approach was taken to sequence and assemble a QTL-rich region, of ~3 Mbp and represented by twenty-seven BACs, on linkage group 5 of the Theobroma cacao cv. Matina 1-6 genome. Using various mixtures of read coverages from paired-end and linear 454 libraries, multiple assemblies of varied quality were generated. Quality was assessed by comparing the assembly of 454 reads with a subset of ten BACs individually sequenced and assembled using Sanger reads. A mixture of reads optimal for assembly was identified. We found, furthermore, that a quality assembly suitable for serving as a reference genome template could be obtained even with a reduced depth of sequencing coverage. Annotation of the resulting assembly revealed several genes potentially responsible for three T. cacao traits: black pod disease resistance, bean shape index, and pod weight. Conclusions Our results, as with other pooled BAC sequencing reports, suggest that pooling portions of a minimum tiling path derived from a BAC-based physical map is an effective method to target sub-genomic regions for sequencing. While we focused on a single QTL region, other QTL regions of importance could be similarly sequenced allowing for biological discovery to take place before a high quality whole-genome assembly is completed.

  8. 论图书馆数字馆藏资源建设中亟待解决的问题%Discussion of Problems in the Construction of the Digital Resources in Libraries

    Institute of Scientific and Technical Information of China (English)

    张德云

    2012-01-01

    In the article the generation, concept and characteristics of the digital resources in libraries were firstly discussed, and the problems in the construction of the digital resources in libraries were analyzed. On the basis of this analysis, the strategies for the construction of the digital resources in libraries were explored.%论述数字馆藏的产生、概念和特点,分析数字馆藏资源建设中存在的问题,探讨数字馆藏资源建设中应采取的策略。

  9. Research on the Construction of Music Resources in University Library%关于普通高校图书馆音乐资源建设的研究

    Institute of Scientific and Technical Information of China (English)

    戴蕾

    2014-01-01

    阐述了普通高校图书馆音乐资源建设的意义,分析了普通高校图书馆音乐资源的现状,提出了普通高校图书馆音乐资源建设的策略。%This paper briefly expounds the significance of the construction of music resources in regular university library,analyzes the current status of music resources in regular university library,and puts forward some strategies for the construction of music resources in regular university library.

  10. Genetic stability of pestivirus genomes cloned into BACs

    DEFF Research Database (Denmark)

    Rasmussen, Thomas Bruun; Reimann, Ilona; Uttenthal, Åse;

    chromosome (BAC) vector “pBeloBAC11”. This BAC vector provides a markedly higher stability of cloned sequences in E. coli compared to plasmids that form the basis for the existing pestivirus cDNA clones. In this study, two of the newly constructed BAC clones were analysed for genetic stability of the cloned...... pestivirus genomes to demonstrate the suitability of the BAC vector for harbouring pestivirus genomes. Two BAC clones, comprising the complete genomes of BDV Gifhorn (pBeloGif3) and CSFV Paderborn (pBeloPader10) were passaged 15 times in E.coli representing at least 360 bacteria generations. From 15th...... passage of the BAC clones, the entire 5’ and 3’ ends of the cloned genomes and parts of the open reading frame were sequenced and compared to the sequences of the parent BAC clones. The sequenced areas represent approximately 20 % of the cloned genome. No mutations were observed after the extensive...

  11. Canadian Library Human Resources Short‐Term Supply and Demand Crisis Is Averted, But a Significant Long‐Term Crisis Must Be Addressed. A review of: 8Rs Research Team. The Future of Human Resources in Canadian Libraries February 2005. Edmonton, AB: University of Alberta. 21 February 2007 .

    OpenAIRE

    Julie McKenna

    2007-01-01

    Objective – To examine the human resources environment in Canadian libraries in order to assess readiness to accommodate change and to identify opportunities for human resources planning. The “8Rs” of the study were defined as recruitment, retirement, retention, remuneration, repatriation, rejuvenation, re‐accreditation, and restructuring.Design – This study was undertaken in three phases over nearly three years through the use a variety of methods including literature review, analyses of exi...

  12. The Use and Maintenance of University Library Electronic Resources%浅议大学图书馆电子资源的利用与维护

    Institute of Scientific and Technical Information of China (English)

    马鹏飞; 熊豫玲

    2011-01-01

    随着科学技术的飞速发展,高新信息技术的广泛应用,大学图书馆的工作内容和方法也面临新的挑战,为了使用户更好的利用大学图书馆的电子资源,提高电子资源利用率,实现大学图书馆数字化,本文主要从电子资源的利用和维护方面进行探讨。%With the rapid development of science and technology,the extensive application of high-tech information technology, University of content and methods of library work is also facing new challenges in order to make better use of university library users to electronic resources,improve the utilization of electronic resources to achieve the University Library digital,paper, from the use of electronic resources and maintenance were discussed.

  13. On the Cloud Storage Mode of University Library Digital Resources%高校图书馆数字资源云存储模式探究

    Institute of Scientific and Technical Information of China (English)

    施蓉蓉

    2012-01-01

    With the rapid increase in quantity of library digital resources,the drawbacks of traditional data storage model increasingly appear.As a new storage service mode,cloud storage provides a new choice for the amount of li-brary digital resources construction.This paper introduces some digital storage technologies,and discusses the application of cloud storage in university library digital resources storage.%随着图书馆数字资源总量的快速增加,传统的数据存储方式弊端日益显现。云存储作为一种新型的存储服务模式,为图书馆的海量数字资源建设提供了一种新的选择。在介绍数字存储技术的基础上,重点探讨了云存储在高校图书馆数字资源存储中的应用。

  14. National BioResource Project-Rat and related activities.

    Science.gov (United States)

    Serikawa, Tadao; Mashimo, Tomoji; Takizawa, Akiko; Okajima, Ryoko; Maedomari, Naoki; Kumafuji, Kenta; Tagami, Fumi; Neoda, Yuki; Otsuki, Mito; Nakanishi, Satoshi; Yamasaki, Ken-ichi; Voigt, Birger; Kuramoto, Takashi

    2009-07-01

    In order to establish a system to facilitate the systematic collection, preservation, and provision of laboratory rats (Rattus norvegicus) and their derivates, the National BioResource Project-Rat (NBRP-Rat) was launched in July 2002. By the end of 2008, more than 500 rat strains had been collected and preserved as live animals, embryos, or sperm. These rat resources are supplied to biomedical scientists in Japan as well as in other countries. This review article introduces NBRP-Rat and highlights the phenome project, recombinant inbred strains, BAC clone libraries, and the ENU-mutant archive, named the Kyoto University Rat Mutant Archive (KURMA). The future direction of rat resources are also discussed.

  15. Are You Overlooking a Valuable Resource? A Practical Guide to Collaborating with Your Greatest Ally: The Public Library

    Science.gov (United States)

    DelGuidice, Margaux

    2009-01-01

    The author's first "real job" when she was fresh out of library school was working as a leave replacement for an elementary school media specialist. One of her first tasks as the interim media specialist was to help organize the fourth grade science fair. Using her recently acquired library school skills, she decided to call the local public…

  16. Web 2.0 as Catalyst: Virtually Reaching Out to Users and Connecting Them to Library Resources and Services

    Science.gov (United States)

    Xiao, Norah

    2008-01-01

    This article is based on services to library users in the area of chemistry at the Science and Engineering (S&E) Library of the University of Southern California (USC), to which I applied various new technologies as outreach approaches. Various Web 2.0 technologies such as a blog, tags, YouTube, RSS feeds, Instant Messaging, online…

  17. Analysis of University Library Digital Resources Acquisition%高校图书馆数字化资源采访探析

    Institute of Scientific and Technical Information of China (English)

    徐爱珍

    2015-01-01

    The development of digital resources affects the business of library work. According to the characteristics of the digital resources, The paper analyzes the influence of digital resources acquisition librarian work and discusses the strategy of acquisition librarian in the digital resource environment.%数字化资源的发展给高校图书馆的各项业务工作带来了影响。文章根据数字化资源的特点,分析了数字化资源对采访馆员业务工作的影响,探讨了数字化资源环境下采访馆员的应对策略。

  18. Genome-wide identification of genes conferring energy related resistance to a synthetic antimicrobial peptide (Bac8c.

    Directory of Open Access Journals (Sweden)

    Eileen C Spindler

    Full Text Available A fundamental issue in the design and development of antimicrobials is the lack of understanding of complex modes of action and how this complexity affects potential pathways for resistance evolution. Bac8c (RIWVIWRR-NH(2 is an 8 amino acid antimicrobial peptide (AMP that has been shown to have enhanced activity against a range of pathogenic Gram-positive and Gram-negative bacteria, as well as yeast. We have previously demonstrated that Bac8c appears to interfere with multiple targets, at least in part through the disruption of cytoplasmic membrane related functions, and that resistance to this peptide does not easily develop using standard laboratory methods. Here, we applied a genomics approach, SCalar Analysis of Library Enrichement (SCALEs, to map the effect of gene overexpression onto Bac8c resistance in parallel for all genes and gene combinations (up to ∼ 10 adjacent genes in the E. coli genome (a total of ∼ 500,000 individual clones were mapped. Our efforts identified an elaborate network of genes for which overexpression leads to low-level resistance to Bac8c (including biofilm formation, multi-drug transporters, etc. This data was analyzed to provide insights into the complex relationships between mechanisms of action and potential routes by which resistance to this synthetic AMP can develop.

  19. A bacterial artificial chromosome library for soybean PI 437654 and identification of clones associated with cyst nematode resistance.

    Science.gov (United States)

    Tomkins, J P; Mahalingam, R; Smith, H; Goicoechea, J L; Knap, H T; Wing, R A

    1999-09-01

    We have constructed a soybean bacterial artificial chromosome (BAC) library using the plant introduction (PI) 437654. The library contains 73 728 clones stored in 192 384-well microtiter plates. A random sampling of 230 BACs indicated an average insert size of 136 kb with a range of 20 to 325 kb, and less than 4% of the clones do not contain inserts. Ninety percent of BAC clones in the library have an average insert size greater than 100 kb. Based on a genome size of 1115 Mb, library coverage is 9 haploid genome equivalents. Screening the BAC library colony filters with cpDNA sequences showed that contamination of the genomic library with chloroplast clones was low (1.85%). Library screening with three genomic RFLP probes linked to soybean cyst nematode (SCN) resistance genes resulted in an average of 18 hits per probe (range 7 to 30). Two separate pools of forward and reverse suppression subtractive cDNAs obtained from SCN-infected and uninfected roots of PI437654 were hybridized to the BAC library filters. The 488 BACs identified from positive signals were fingerprinted and analyzed using FPC software (version 4.0) resulting in 85 different contigs. Contigs were grouped and analyzed in three categories: (1) contigs of BAC clones which hybridized to forward subtracted cDNAs, (2) contigs of BAC clones which hybridized to reverse subtracted cDNAs, and (3) contigs of BAC clones which hybridized to both forward and reverse subtracted cDNAs. This protocol provides an estimate of the number of genomic regions involved in early resistance response to a pathogenic attack.

  20. Construction and Identification of Bacterial Artificial Chromosome Library for 0-613-2R in Upland Cotton

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    A bacterial artificial chromosome (BAC) library containing a large genomic DNA insert is an important tool for genome physical mapping, map-based cloning, and genome sequencing. To isolate genes via a map-based cloning strategy and to perform physical mapping of the cotton genome, a high-quality BAC library containing large cotton DNA inserts is needed. We have developed a BAC library of the restoring line 0-613-2R for isolating the fertility restorer (Rf1) gene and genomic research in cotton (Gossypium hirsutum L.). The BAC library contains 97 825 clones stored in 255 pieces of a 384-well microtiter plate. Random samples of BACs digested with the Notl enzyme indicated that the average insert size is approximately 130 kb, with a range of 80-275 kb,and 95.7% of the BAC clones in the library have an average insert size larger than 100 kb. Based on a cotton genome size of 2 250 Mb, library coverage is 5.7 x haploid genome equivalents. Four clones were selected randomly from the library to determine the stability of the BAC clones. There were no different fingerprints for 0 and 100 generations of each clone digested with Notl and Hindlll enzymes. Thus, the stability of a single BAC clone can be sustained at least for 100 generations. Eight simple sequence repeat (SSR) markers flanking the Rf1 gene were chosen to screen the BAC library by pool using PCR method and 25 positive clones were identified with 3.1 positive clones per SSR marker.

  1. Printed Resources and Electronic Resources Integrated Services in Small and Medium-sized Libraries%中小型图书馆印本资源与电子资源一体化服务实践

    Institute of Scientific and Technical Information of China (English)

    刘胜

    2014-01-01

    Through analyzing the integrated service system of library resources, this paper sums up three methods for the integration of printed resources and digital resources including the digital resources catalog, digital resources navigate and open link system, and suggests that the libraries should give priority to books and periodicals integrated retrieval in the integrated service, and carry out the system construction within their capabilities by adopting the methods of making second development, introducing new system, and conducting independent development, etc.%通过对图书馆馆藏一体化服务系统的分析,归纳总结出印本资源与电子资源整合的3种主要模式———电子资源编目、电子资源导航与开放链接,并建议图书馆在进行一体化服务时,优先考虑图书和期刊的整合检索,在系统建设上量力而行,采取二次开发、引进新系统、自主开发等方式。

  2. Chromosome region-specific libraries for human genome analysis. Final progress report, 1 March 1991--28 February 1994

    Energy Technology Data Exchange (ETDEWEB)

    Kao, F.T.

    1994-04-01

    The objectives of this grant proposal include (1) development of a chromosome microdissection and PCR-mediated microcloning technology, (2) application of this microtechnology to the construction of region-specific libraries for human genome analysis. During this grant period, the authors have successfully developed this microtechnology and have applied it to the construction of microdissection libraries for the following chromosome regions: a whole chromosome 21 (21E), 2 region-specific libraries for the long arm of chromosome 2, 2q35-q37 (2Q1) and 2q33-q35 (2Q2), and 4 region-specific libraries for the entire short arm of chromosome 2, 2p23-p25 (2P1), 2p21-p23 (2P2), 2p14-p16 (wP3) and 2p11-p13 (2P4). In addition, 20--40 unique sequence microclones have been isolated and characterized for genomic studies. These region-specific libraries and the single-copy microclones from the library have been used as valuable resources for (1) isolating microsatellite probes in linkage analysis to further refine the disease locus; (2) isolating corresponding clones with large inserts, e.g. YAC, BAC, P1, cosmid and phage, to facilitate construction of contigs for high resolution physical mapping; and (3) isolating region-specific cDNA clones for use as candidate genes. These libraries are being deposited in the American Type Culture Collection (ATCC) for general distribution.

  3. 谈地方高校图书馆数字资源建设%Discussion on the Construction of Digital Resources in Local University Library

    Institute of Scientific and Technical Information of China (English)

    焦玲霞

    2011-01-01

    通过对数字资源投资比重与不同载体资源的获取行为的分析可知,数字资源与纸质资源获取行为的趋势呈反向关系;数字资源投资比重越大,两载体资源的获取分化趋势就越明显。调研结果表明,高校图书馆应适当控制数字资源投资比重,降低对数字资源的依赖性;引进数字资源需量力而行;在增加数字资源投资比重的同时,应同步提高数字资源的服务质量。%Through the analysis on the investment ratio of digital resources and readers' acquirement actions of different media,we can find that the trend for acquiring digital resources and printed resources are opposite;as the investment ratio of digital resources becomes larger,the differentiation trends of digital resources and printed resources is more obvious.Results of survey study show that university libraries should control the investment ratio of digital resources moderately,in order to reduce the dependence on digital resources;acquire digital resources within libraries' capabilities;improve the service quality of digital resources when increasing the investment ratio of digital resources.

  4. 浅谈公共图书馆的人力资源开发与管理%On the Development and Management of Human Resources at Public Libraries

    Institute of Scientific and Technical Information of China (English)

    许迎霞

    2012-01-01

    提出了新世纪对图书馆员素质要求,图书馆应该在日常工作中开发人力资源,要加强人力资源管理,要优化人员结构,建立公平竞争机制,引入激励机制,强化考核手段,建立有效的培训机制,以图书馆文化感染人,制定合理、有效的人力资源开发与管理规则,以实现人力资源管理的科学化、规范化和民主化。%The paper according to the demand of quality of librarians in the new century, The author Proposed the library should develop the human resources in the routine work, Must strengthen the human resources management, must optimize the personnel structure, establishes the fair competition mechanism, the introduction drives the mecha- nism, the strengthened inspection method, establishes the effective training mechanism, by library culture infection person, formulation reasonable, effective human resources development and management rule, realizes the human resources management scientific style, the standardization and the democratization

  5. Public librarians as a resource for promoting health: results from the Health for Everyone in Libraries Project (HELP) librarian survey.

    Science.gov (United States)

    Linnan, Laura A; Wildemuth, Barbara M; Gollop, Claudia; Hull, Peggy; Silbajoris, Christie; Monnig, Ruth

    2004-04-01

    Public libraries are located in all communities, and two thirds of adults visit one each year. Libraries give the public access to computers and the Internet, and librarians offer technical assistance for accessing information. The interests and training needs of public librarians for assisting the public in accessing health information have not been addressed. One public library/librarian in each North Carolina county was randomly selected to complete a written questionnaire to assess health-related information services and librarians' skills for providing these services. 84% of librarians (83/99) completed the questionnaire. Results indicate that librarians answer more than 10 health-related questions per week, feel moderately comfortable answering these questions, and are very interested in receiving additional training for addressing health-related questions. Creating public library/public health partnerships holds much promise for enhancing the ability of community members to access desired health information.

  6. Library Skills Workbook.

    Science.gov (United States)

    Miller, Constance; And Others

    This self-paced library skills workbook provides incoming students with an introduction to the physical arrangement and use of the university libraries. Divided into two sections, the first contains explanations of the catalogs, classification system, and various library resources. The second consists of practical exercises keyed to the…

  7. Biology Library Workbook.

    Science.gov (United States)

    Miller, Constance; And Others

    A library skills workbook provides college biology students with an introduction to biological library resources. Divided into two sections, the first contains explanations of the various steps in the library research process. The second consists of exercises keyed to the explanatory chapters of the first section. (RAA)

  8. Changing State Digital Libraries

    Science.gov (United States)

    Pappas, Marjorie L.

    2006-01-01

    Research has shown that state virtual or digital libraries are evolving into websites that are loaded with free resources, subscription databases, and instructional tools. In this article, the author explores these evolving libraries based on the following questions: (1) How user-friendly are the state digital libraries?; (2) How do state digital…

  9. Analysis on the Utilization of Digital Resources in Military Academy Library%军校图书馆数字资源利用现状分析

    Institute of Scientific and Technical Information of China (English)

    王宏燕

    2016-01-01

    随着社会科学技术的不断发展和教育机制的创新改革,越来越多的电子及网络开始出版,各大高校的图书馆资源也逐渐变得数字化,传统纸质图书馆已不能够满足学生的要求。文章分析了各军校图书馆目前存在的数字资源利用问题。%With the development of society, science and technology and education mechanism of reform and innovation, more and more electronic and network began publishing. Major colleges and universities library resources have gradually become digitized, so the traditional paper libraries cannot meet the students ’ more requirements. The paper researches on the existing problems in various military academy library digital resources utilization process.

  10. Library Information Resource Integration System Study%图书馆信息资源整合系统的探究

    Institute of Scientific and Technical Information of China (English)

    郑剑

    2011-01-01

    随着数字图书馆建设进程的加快,各种类型、格式的数字资源急剧增加。但是因为数字资源的异构和分散、数据内容的交叉和重复、检索方式的多样性等等,致使用户面对浩瀚的信息海洋,往往无所适从,不知从何入手获取所需信息,他们不得不费很多时间学习各种数据库的使用方法、检索策略等。这样给用户造成很大的负担,并且严重影响了资源的利用率。为了解决这个问题,国内外图书馆都在积极开展数字资源整合工作,并已成为数字图书馆建设中一项迫切且艰巨的任务。鉴于资源整合工作对图书馆发展的重要性,数字资源整合研究已成为现阶段图书馆界研究的热门话题。在这样一个集群化的数字图书馆,具体的存贮介质与服务器的分布已经不重要,而重要的是数字图书馆所提供的服务与系统的功能。%With the acceleration of the process of building digital libraries,of all types and formats of digital resources has increased dramatically.But because the number of heterogeneous and distributed resources,data,content overlap and duplication,the diversity of retrieval methods,etc.,causing the user to face the vast ocean of information, often at a loss,I do not know where to start to obtain the required information,they have to charge a lot of time learning to use various databases, search strategies.This caused a great burden to the user, and a serious impact on resource utilization. To solve this problem, the library at home and abroad are actively working to carry out the integration of digital resources and digital library construction has become a pressing and arduous task.Given the integration of library resources,the importance of development,the integration of digital resources at this stage has become a hot topic in research libraries./n such a cluster of digital libraries, storage media and server specific distribution is not

  11. Genomic insight into the common carp (Cyprinus carpio genome by sequencing analysis of BAC-end sequences

    Directory of Open Access Journals (Sweden)

    Wang Jintu

    2011-04-01

    Full Text Available Abstract Background Common carp is one of the most important aquaculture teleost fish in the world. Common carp and other closely related Cyprinidae species provide over 30% aquaculture production in the world. However, common carp genomic resources are still relatively underdeveloped. BAC end sequences (BES are important resources for genome research on BAC-anchored genetic marker development, linkage map and physical map integration, and whole genome sequence assembling and scaffolding. Result To develop such valuable resources in common carp (Cyprinus carpio, a total of 40,224 BAC clones were sequenced on both ends, generating 65,720 clean BES with an average read length of 647 bp after sequence processing, representing 42,522,168 bp or 2.5% of common carp genome. The first survey of common carp genome was conducted with various bioinformatics tools. The common carp genome contains over 17.3% of repetitive elements with GC content of 36.8% and 518 transposon ORFs. To identify and develop BAC-anchored microsatellite markers, a total of 13,581 microsatellites were detected from 10,355 BES. The coding region of 7,127 genes were recognized from 9,443 BES on 7,453 BACs, with 1,990 BACs have genes on both ends. To evaluate the similarity to the genome of closely related zebrafish, BES of common carp were aligned against zebrafish genome. A total of 39,335 BES of common carp have conserved homologs on zebrafish genome which demonstrated the high similarity between zebrafish and common carp genomes, indicating the feasibility of comparative mapping between zebrafish and common carp once we have physical map of common carp. Conclusion BAC end sequences are great resources for the first genome wide survey of common carp. The repetitive DNA was estimated to be approximate 28% of common carp genome, indicating the higher complexity of the genome. Comparative analysis had mapped around 40,000 BES to zebrafish genome and established over 3

  12. Psychological Analysis of Public Library Readers in the Environment of Computer Network Resources%计算机网络资源环境下公共图书馆读者心理分析

    Institute of Scientific and Technical Information of China (English)

    梁佳

    2012-01-01

    Public libraries as a nonprofit cultural educational institution,is open for the public,all members of society who use the library resources,library services object.The Public Library of the nature of its readers with a wide range of social and mass characteristics.Understanding of computer network resources and the environment,public library readers psychology,master of readers of different ages tend to provide a basis for public library services.Public Library audience composition and reading motivation and purpose,read the psychological differences.Readers 'psychology,and the corresponding collection of literature data and network information resources services to meet readers' needs to improve the collection of documents and network resource utilization,of great significance for readers of different age groups.The computer network resources and the environment,public library readers psychological to make analysis,to further explore the computer network resources and the environment,how public libraries should better carry out the reader service work.%公共图书馆作为公益性文化教育机构,是面向社会公众开放的,凡是具有利用图书馆资源条件的一切社会成员,都是图书馆服务对象。公共图书馆的性质决定了它的读者具有广泛的社会性和群众性特点。了解计算机网络资源环境下公共图书馆的读者心理,掌握不同年龄读者的阅读倾向,能为公共图书馆读者服务工作提供依据。

  13. Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species

    Directory of Open Access Journals (Sweden)

    Doležel Jaroslav

    2010-02-01

    Full Text Available Abstract Background The presence of closely related genomes in polyploid species makes the assembly of total genomic sequence from shotgun sequence reads produced by the current sequencing platforms exceedingly difficult, if not impossible. Genomes of polyploid species could be sequenced following the ordered-clone sequencing approach employing contigs of bacterial artificial chromosome (BAC clones and BAC-based physical maps. Although BAC contigs can currently be constructed for virtually any diploid organism with the SNaPshot high-information-content-fingerprinting (HICF technology, it is currently unknown if this is also true for polyploid species. It is possible that BAC clones from orthologous regions of homoeologous chromosomes would share numerous restriction fragments and be therefore included into common contigs. Because of this and other concerns, physical mapping utilizing the SNaPshot HICF of BAC libraries of polyploid species has not been pursued and the possibility of doing so has not been assessed. The sole exception has been in common wheat, an allohexaploid in which it is possible to construct single-chromosome or single-chromosome-arm BAC libraries from DNA of flow-sorted chromosomes and bypass the obstacles created by polyploidy. Results The potential of the SNaPshot HICF technology for physical mapping of polyploid plants utilizing global BAC libraries was evaluated by assembling contigs of fingerprinted clones in an in silico merged BAC library composed of single-chromosome libraries of two wheat homoeologous chromosome arms, 3AS and 3DS, and complete chromosome 3B. Because the chromosome arm origin of each clone was known, it was possible to estimate the fidelity of contig assembly. On average 97.78% or more clones, depending on the library, were from a single chromosome arm. A large portion of the remaining clones was shown to be library contamination from other chromosomes, a feature that is unavoidable during the

  14. 数字图书馆资源配置及优化探究%Probe into the Configuration and Optimization of Digital Library ’s Resources

    Institute of Scientific and Technical Information of China (English)

    刘洪岩; 阮红

    2015-01-01

    Under normal circumstances, digital library’s resources refer mainly to digital books’ content resources. Along with the rapid development of digital library, the quantity of digital books resources are increasingly large. How to configurate and optimize the massive digital resources has become become a pressing problem facing the current development of digital library. According to the cases at home and abroad, and connecting with the working practice, this paper probes into the configuration and optimization of digital library’s content resources from three angles of the positioning, copyright and resource integration, and puts forward some new views and ideas.%数字图书馆资源一般情况下主要是指数字图书的内容资源。随着数字图书馆的快速发展,数字图书资源数量日渐庞大,如何对海量的数字资源进行优化配置,成为当前数字图书馆发展所面临的紧迫问题。根据国内外案例,结合从业实践,从定位、版权和资源整合3个角度,探讨了当前数字图书馆内容资源的配置与优化问题,并提出了全新的看法与思路。

  15. Challenges and Opportunities for Libraries in Pakistan

    OpenAIRE

    Shafiq UR, Rehman; Pervaiz, Ahmad

    2007-01-01

    Abstract: This paper, based on review of literature, observation, and informal conversations, discusses various challenges regarding finance, collection development, ICTs, human resources, library education, library association and research & development faced by library profession in Pakistan. The opportunities to meet these challenges have also been explored. Keywords: Library challenges and opportunities (Pakistan); Librarianship (Pakistan); Library issues; Library profession in Pa...

  16. Library catalogues as resources for book history: case study of Novosel’s bookstore catalogue in Zagreb (1794 - 1825

    Directory of Open Access Journals (Sweden)

    Marijana Tomić

    2008-07-01

    Full Text Available The aim of the paper is to analyze the book catalogue of Novosel’s bookstore, which operated in Zagreb from 1794 to 1825, and investigate the history of books and writing in Zagreb at the turn of the 19th century. The catalogue we analyzed is believed to have been published in 1801. Bearing in mind that the market-based economy started to develop in the late 18th century, it can be stipulated that Novosel and his staff and successors based the offer in their bookstore on market analysis, i.e. their readers’ needs. The increase in offer has sparked off new advertising techniques, i.e. printing of catalogues. It follows that their book catalogue reflects the image of the cultural and intellectual status and needs of readers in those times. The paper provides a short overview of book trade in the late 18th century Zagreb and of bookstore advertisements published both in books and individually, as well as a short overview of Novosel’s bookstore business. In the analysis we partly use the methodology introduced by Robert Darnton, the so-called Darnton’s circle, which takes a holistic view of the history of books taking into consideration all stages a book needs to go through - from the author, publisher, printer, bookstores, to readers, including the author him/herself as a reader. Every element is considered in relation to other elements in the circle, and in connection with external factors such as the economic and social environment, and political and intellectual influences. The books presented in the catalogue have been analyzed using different criteria: language, genre and country where they were printed. Books printed in Croatia and those written in Croatian have been given priority. In the catalogue analysis we used the database Skupni katalog hrvatskih knjižnica (joint Croatian library catalogue in order to reconstruct the printing year and printing shops that have not been listed in the catalogues. Using this methodology, we partly

  17. Library news

    CERN Multimedia

    CERN Library

    2010-01-01

    The CERN Library has been providing electronic access to the "Techniques de l'Ingénieur" database for the past 8 months. As a reminder, this is a multidisciplinary database of over 4000 technical and scientific articles in French, covering a broad range of topics such as mechanical engineering, safety, electronics and the environment. In a few simple steps, you can create your own account, select the types of documents you are interested in and configure your settings so as to receive alerts when articles in your field of activity are published. You can now access this resource from outside CERN using the "remote access to electronic resources" service. Further information is available here. Direct access to the database. Remote access to electronic resources. If you have any questions or comments, don't hesitate to contact us at: library.desk@cern.ch.

  18. 高校图书馆数字资源的采购及评价研究%Study on the Purchase and Evaluation of Digital Resources in University Library

    Institute of Scientific and Technical Information of China (English)

    郑兵

    2015-01-01

    目前,数字资源在高校图书馆馆藏中所占比重越来越大,已经成为图书馆资源的重要组成部分.介绍了高校图书馆数字资源采购的特点与流程,对数字资源的使用评价指标进行了探讨.%At present,the proportion of the digital resources in university library's collection is more and more,and the digital resources have become an important component of library's resources.This paper introduces the features and process of the purchase of university library's digital resources,and probes into the application evaluation indexes of the digital resources.

  19. Expression Analysis of CB2-GFP BAC Transgenic Mice.

    Science.gov (United States)

    Schmöle, Anne-Caroline; Lundt, Ramona; Gennequin, Benjamin; Schrage, Hanna; Beins, Eva; Krämer, Alexandra; Zimmer, Till; Limmer, Andreas; Zimmer, Andreas; Otte, David-Marian

    2015-01-01

    The endocannabinoid system (ECS) is a retrograde messenger system, consisting of lipid signaling molecules that bind to at least two G-protein-coupled receptors, Cannabinoid receptor 1 and 2 (CB1 and 2). As CB2 is primarily expressed on immune cells such as B cells, T cells, macrophages, dendritic cells, and microglia, it is of great interest how CB2 contributes to immune cell development and function in health and disease. Here, understanding the mechanisms of CB2 involvement in immune-cell function as well as the trafficking and regulation of CB2 expressing cells are crucial issues. Up to now, CB2 antibodies produce unclear results, especially those targeting the murine protein. Therefore, we have generated BAC transgenic GFP reporter mice (CB2-GFPTg) to trace CB2 expression in vitro and in situ. Those mice express GFP under the CB2 promoter and display GFP expression paralleling CB2 expression on the transcript level in spleen, thymus and brain tissue. Furthermore, by using fluorescence techniques we show that the major sources for GFP-CB2 expression are B cells in spleen and blood and microglia in the brain. This novel CB2-GFP transgenic reporter mouse line represents a powerful resource to study CB2 expression in different cell types. Furthermore, it could be used for analyzing CB2-mediated mobilization and trafficking of immune cells as well as studying the fate of recruited immune cells in models of acute and chronic inflammation.

  20. Expression Analysis of CB2-GFP BAC Transgenic Mice.

    Directory of Open Access Journals (Sweden)

    Anne-Caroline Schmöle

    Full Text Available The endocannabinoid system (ECS is a retrograde messenger system, consisting of lipid signaling molecules that bind to at least two G-protein-coupled receptors, Cannabinoid receptor 1 and 2 (CB1 and 2. As CB2 is primarily expressed on immune cells such as B cells, T cells, macrophages, dendritic cells, and microglia, it is of great interest how CB2 contributes to immune cell development and function in health and disease. Here, understanding the mechanisms of CB2 involvement in immune-cell function as well as the trafficking and regulation of CB2 expressing cells are crucial issues. Up to now, CB2 antibodies produce unclear results, especially those targeting the murine protein. Therefore, we have generated BAC transgenic GFP reporter mice (CB2-GFPTg to trace CB2 expression in vitro and in situ. Those mice express GFP under the CB2 promoter and display GFP expression paralleling CB2 expression on the transcript level in spleen, thymus and brain tissue. Furthermore, by using fluorescence techniques we show that the major sources for GFP-CB2 expression are B cells in spleen and blood and microglia in the brain. This novel CB2-GFP transgenic reporter mouse line represents a powerful resource to study CB2 expression in different cell types. Furthermore, it could be used for analyzing CB2-mediated mobilization and trafficking of immune cells as well as studying the fate of recruited immune cells in models of acute and chronic inflammation.

  1. Estimating P-coverage of biosynthetic pathways in DNA libraries and screening by genetic selection: biotin biosynthesis in the marine microorganism Chromohalobacter.

    Science.gov (United States)

    Kim, Eun Jin; Angell, Scott; Janes, Jeff; Watanabe, Coran M H

    2008-06-01

    Traditional approaches to natural product discovery involve cell-based screening of natural product extracts followed by compound isolation and characterization. Their importance notwithstanding, continued mining leads to depletion of natural resources and the reisolation of previously identified metabolites. Metagenomic strategies aimed at localizing the biosynthetic cluster genes and expressing them in surrogate hosts offers one possible alternative. A fundamental question that naturally arises when pursuing such a strategy is, how large must the genomic library be to effectively represent the genome of an organism(s) and the biosynthetic gene clusters they harbor? Such an issue is certainly augmented in the absence of expensive robotics to expedite colony picking and/or screening of clones. We have developed an algorism, named BPC (biosynthetic pathway coverage), supported by molecular simulations to deduce the number of BAC clones required to achieve proper coverage of the genome and their respective biosynthetic pathways. The strategy has been applied to the construction of a large-insert BAC library from a marine microorganism, Hon6 (isolated from Honokohau, Maui) thought to represent a new species. The genomic library is constructed with a BAC yeast shuttle vector pClasper lacZ paving the way for the culturing of libraries in both prokaryotic and eukaryotic hosts. Flow cytometric methods are utilized to estimate the genome size of the organism and BPC implemented to assess P-coverage or percent coverage. A genetic selection strategy is illustrated, applications of which could expedite screening efforts in the identification and localization of biosynthetic pathways from marine microbial consortia, offering a powerful complement to genome sequencing and degenerate probe strategies. Implementing this approach, we report on the biotin biosynthetic pathway from the marine microorganism Hon6.

  2. 顺德图书馆数字资源服务实践探索%Exploration for the Digital Resources Service in Shunde Library

    Institute of Scientific and Technical Information of China (English)

    萧志华

    2012-01-01

    Taking Shunde Library as an example, this paper analyzes the problem of digital resources service in the past and summarizes the current innovation of digital resource service. Besides, this paper also discusses the problems that need to be solved on the development of digital resources service.As an important part of public digital services in Shunde District, Shunde Library should not only serve all people in Shunde, but also provide public digital services in a all-round way with other public service organizations.%文章以顺德图书馆为例,分析过去数字资源服务存在的问题,总结当前数字资源服务的创新实践,就今后数字资源服务需要解决的问题进行探讨,并提出作为顺德地区公共数字服务的一个重要的组成部分,顺德图书馆不仅要在服务广度上让所有的市民受惠,也要与其他公共服务机构联合起来,提供全方位的公共数字文化服务.

  3. Discussion on Literature Resource Construction of Hospital Libraries%医院图书馆文献资源建设探讨

    Institute of Scientific and Technical Information of China (English)

    蔺艺红; 赵雯; 王敏; 郭华; 安鹏

    2011-01-01

    The construction of literature resource is the fondation of hospital libraries' every work, the quality of the construction of literature resource affects the service of the libraries directly.In order to do well the construction of literature resource, we need to correctly handle four relationshipes such as the relationship between paper documents and electronic documents, the relationship between featured collection and general collection,the relationship between reality collection and virtual collection, and the relationship between possession and access.%文献资源建设是医院图书馆开展一切工作的基础,文献资源建设的好坏直接影响图书馆的服务质量.要做好文献资源建设首先应该正确处理好 (1)纸质文献和电子文献的关系;(2)特色馆藏与一般馆藏的关系;(3)现实馆藏与虚拟馆藏的关系;(4)拥有与存取的关系.

  4. A new method for rapidly generating gene-targeting vectors by engineering BACs through homologous recombination in bacteria.

    Science.gov (United States)

    Cotta-de-Almeida, Vinicius; Schonhoff, Susan; Shibata, Tomoyuki; Leiter, Andrew; Snapper, Scott B

    2003-09-01

    Generating knockout mice is still an expensive and highly time-consuming process. Target construct generation, the first labor-intensive step in this process, requires the manipulation of large fragments of DNA and numerous, and often cumbersome, cloning steps. Here we show the development of a rapid approach for generating targeting constructs that capitalizes on efficient homologous recombination between linear DNA fragments and circular plasmids in Escherichia coli ("recombineering"), the availability of bacterial artificial chromosomes (BACs), and the accessibility of the sequence of the mouse genome. Employing recombineering, we demonstrate with only 1-2 template plasmids, short homologies (40-50bp) between donor and target DNA, and one subcloning step that we can efficiently manipulate BACs in situ to generate a complicated targeting vector. This procedure avoids the need to construct or screen genomic libraries and permits the generation of most standard, conditional, or knock-in targeting vectors, often within two weeks.

  5. BACs as tools for the study of genomic imprinting.

    Science.gov (United States)

    Tunster, S J; Van De Pette, M; John, R M

    2011-01-01

    Genomic imprinting in mammals results in the expression of genes from only one parental allele. Imprinting occurs as a consequence of epigenetic marks set down either in the father's or the mother's germ line and affects a very specific category of mammalian gene. A greater understanding of this distinctive phenomenon can be gained from studies using large genomic clones, called bacterial artificial chromosomes (BACs). Here, we review the important applications of BACs to imprinting research, covering physical mapping studies and the use of BACs as transgenes in mice to study gene expression patterns, to identify imprinting centres, and to isolate the consequences of altered gene dosage. We also highlight the significant and unique advantages that rapid BAC engineering brings to genomic imprinting research.

  6. BACs as Tools for the Study of Genomic Imprinting

    Directory of Open Access Journals (Sweden)

    S. J. Tunster

    2011-01-01

    Full Text Available Genomic imprinting in mammals results in the expression of genes from only one parental allele. Imprinting occurs as a consequence of epigenetic marks set down either in the father's or the mother's germ line and affects a very specific category of mammalian gene. A greater understanding of this distinctive phenomenon can be gained from studies using large genomic clones, called bacterial artificial chromosomes (BACs. Here, we review the important applications of BACs to imprinting research, covering physical mapping studies and the use of BACs as transgenes in mice to study gene expression patterns, to identify imprinting centres, and to isolate the consequences of altered gene dosage. We also highlight the significant and unique advantages that rapid BAC engineering brings to genomic imprinting research.

  7. Construction and prospects of parasite germplasm resources library in China%中国寄生虫种质资源库的构建与展望

    Institute of Scientific and Technical Information of China (English)

    陈韶红; 周晓农; 胡薇; 沈海默; 张仪; 陈家旭; 李浩; 张永年; 周卉; 童小妹

    2013-01-01

    目的 通过对各种寄生虫种质资源的收集、整理、鉴定和保藏,构建中国寄生虫种质资源库.方法 根据各种寄生虫虫种资源的生物学特性,制定寄生虫虫种资源共性描述标准和各类寄生虫个性描述标准及技术规范,对动物、植物和人体寄生虫进行标准化分类整理、保藏,并进行数字化描述,建立统一规范的实物库和数据库.结果 建立了含有11个门23个纲1 115种/117 814件寄生虫种质资源实物库和数据库;构建了3个寄生虫种质资源活体保藏基地;实物保藏库波及全国15个地区,发现了11种新溪蟹种和1种线虫新种;构建了中国寄生虫种资源网(www.psic.net.cn).结论 初步建立了中国寄生虫种质资源库.%We aim to establish the parasite germplasm resources library by collecting, sorting, identifying and conserving various parasites in China. According to biological characteristics of a variety of parasite, description standards and technical specifications on both commonness and personality of parasites were established. Moreover, standardized sorting, preservation and digitized description of animal, plant and human parasites were also conducted in order to establish a unified and standardized physical library and database. The parasite germplasm resources physical library and database were established successfully, which contained 117 814 parasite germplasms covering 1 115 species in 23 classes in 11 phylum. Three preservation bases of parasite germplasm resources for living samples were constructed and the physical preservation libraries involve 15 provinces across the whole country. A total of 11 new brook crab species and one kind of worm species were founded. The website of China parasites resource network (www. psic. net. cn) has also been launched.

  8. Toy Library and International Toy Library Association

    OpenAIRE

    Kamaraj, Işık

    2013-01-01

    Toy libraries are resource centers that provides assistance to young children and theirfamilies, gives consultancy service and information about plays, offers play materials andeducational activities and supplies appropriate materials and toys for child development aswell as contributing the development of children. Toy libraries are in commission all over theworld since 1935. The first toy library is opened in America, then in Sweden, England,Canada and Australia respectively. Today, there a...

  9. Larger, Higher-level Academic Institutions in the US Do Not Necessarily Have Better-resourced Library Web Teams. A Review of: Connell, Ruth Sara.

    Directory of Open Access Journals (Sweden)

    Suzanne Lewis

    2008-12-01

    -year colleges more likely (91.7% to share a Web team than four-year or above institutions (60.9%. The majority of responding institutions (94.4% used in-house library Web site design, with only 5.6% of respondents outsourcing this task.Nearly half (49% of respondents indicated that library Web design was done by one person and even the larger libraries did not necessarily have larger Web teams. Very few Web team members (4.9% had Web design as their primary role; the majority (83.5% indicated that it was just one component of their job. Web team members from master’s- and doctorate-granting institutions were more likely to have taught themselves Web design, while those from associate, baccalaureate and special focus colleges were more likely to have taken Web design courses. For all respondents, the most commonly listed quality for selection to the Web team was an interest in Web design and the most valued skill for library Web designers was the ability to organise information effectively.Knowledge of Web authoring software and basic HTML coding were the most commonly listed knowledge requirements for Web team members. A significant number of respondents indicated that they or other Web team members did not have access to Web authoring (36.9% and image editing (52% software. Generally (except for two very large institutions, the larger institutions were more likely to use database-driven systems for their library Web sites and the smaller institutions were more likely to use content management systems. Associate’s and special focus colleges were less likely than other types of institutions to use either database driven or content management systems. Associate’s institutions were more likely to achieve ADA (Americans with Disabilities Act Web accessibility compliance. Only 48.6% of respondents utilised usability testing during Web site design.Conclusion – The author expected that institutions providing higher levels of education would have better-resourced Web design and

  10. 信息资源地域配置与图书馆馆藏结构的确定%Allocation of Information Resources According to Regions & Determination of the Structure of Library Collection

    Institute of Scientific and Technical Information of China (English)

    刘建国; 范亚

    2003-01-01

    The paper analyzes the factors that influence the allocation of information resources in accordance with theregions. It describes how to allocate information resources in a balanced way. Principles and measures by which we canbuild a rational suructure of library collection are also given.

  11. Paths of Discovery: Comparing the Search Effectiveness of EBSCO Discovery Service, Summon, Google Scholar, and Conventional Library Resources

    Science.gov (United States)

    Asher, Andrew D.; Duke, Lynda M.; Wilson, Suzanne

    2013-01-01

    In 2011, researchers at Bucknell University and Illinois Wesleyan University compared the search efficacy of Serial Solutions Summon, EBSCO Discovery Service, Google Scholar, and conventional library databases. Using a mixed-methods approach, qualitative and quantitative data were gathered on students' usage of these tools. Regardless of the…

  12. The Discussion of the Construction of the University Library Digital Resource%高校图书馆数字资源建设刍议

    Institute of Scientific and Technical Information of China (English)

    王令祎

    2014-01-01

    数字资源建设已经成为高校图书馆文献资源建设的重要组成部分,但许多深层次原因阻碍着高校数字资源建设的脚步。本文从数字资源重复建设、推广普及度、知识产权保护问题、馆际协作、数字资源建设标准和使用评价系统六个方面进行探讨,为问题的解决提出一些思路。%Summary: the construction of the digital resource has become the most important part of University li-brary's literature resources construction, but there still has many Underlying reasons which put off the development of the construction of digital resources. This paper will discuss and show some ideas on six questions, which are the construction of digital resources, popularization of it, intellectual property protection problems, interlibrary coopera-tion, the construction standards of digital resources, and evaluation system of users.

  13. 当前高校图书馆人力资源管理问题研究%Study on Human Resource Management of Current University Library

    Institute of Scientific and Technical Information of China (English)

    刘宏

    2012-01-01

    Taking knowledge-based economy environment as the background, the thesis discussed the current status of human resources in university libraries and the problems, and made countermeasures and proposals for human resources optimization from the concept, planning, environment, configuration, training, performance appraisal, culture and other aspects.%以知识经济环境下为背景,讨论了当前高校图书馆人力资源现状及存在的问题,提出了从观念、规划、环境、配置、培训、绩效考核、文化等环节优化人力资源的对策建议.

  14. Canadian Library Human Resources Short‐Term Supply and Demand Crisis Is Averted, But a Significant Long‐Term Crisis Must Be Addressed. A review of: 8Rs Research Team. The Future of Human Resources in Canadian Libraries February 2005. Edmonton, AB: University of Alberta. 21 February 2007 .

    Directory of Open Access Journals (Sweden)

    Julie McKenna

    2007-03-01

    Full Text Available Objective – To examine the human resources environment in Canadian libraries in order to assess readiness to accommodate change and to identify opportunities for human resources planning. The “8Rs” of the study were defined as recruitment, retirement, retention, remuneration, repatriation, rejuvenation, re‐accreditation, and restructuring.Design – This study was undertaken in three phases over nearly three years through the use a variety of methods including literature review, analyses of existing data (Statistics Canada and library school graduate data, telephone interviews (with senior library administrators, focus groups (with representatives from Canadian Association of Research Libraries, Canadian Urban Libraries Council and Alberta Association of Library Technicians, print surveys (library institutions and web‐based surveys (of professional librarians and paraprofessional library staff.Setting – Canadian libraries that are not component branches of a system, and that employ professional librarians.Subjects – Stage I: 17 senior library administrators participated in telephone interviews and three focus groups were conducted. Stage II: Surveyed library administrators representing institutions. A multi‐stage stratified random sampling technique was used to ensure geographical representation from each of Canada’s provinces and territories. Full census participation was conducted for members of the Canadian Association of Research Libraries and the Canadian Urban Libraries Council. The print survey instrument was distributed to 1,357 subjects; 461 completed surveys were returned (response rate of 34% with results for the total sample accurate within plus or minus 3.8 per centage points, 95 times out of 100. Stage III: Surveyed professional librarians and paraprofessional staff. Multi‐stage random sampling was used to ensure representation of library staff from all library sectors and sufficient sub‐sample sizes. Of the

  15. [Participation of the antibiotics of Bac. pumilus and Bac. subtilis in the regulation of bacterial spore formation].

    Science.gov (United States)

    Lukin, A A; Korolev, V I

    1979-03-01

    Sporulation and antibiotic production, as well as the effect of exogenic antibacterial substances on bacterial sporogenesis were studied in various strains of Bac. pumilus and Bac. pumilus and Bac. subtilis. The bacteria were grown on a solid sporulation medium with and without the antibiotics. After 5-day incubation the presence of refractyl spores was determined with a phase-contrast method. It was found that in the strains of Bac. pumilus producing antibacterial substances the sporulation was normal. The loss of the capacity for synthesizing such substances resulted in asporegenicity or oligosporogenicity. This allowed a conclusion on existence of phenomenological connection between sporulation and antibiotic production. The study of the antibiotic effect on bacterial sporogenesis showed negative results which are discussed in the paper along two directions: (1) the antibiotics did not probably participate in regulation of the bacteria cell differentiation, (2) the antibiotics regulated the bacterial sporogenesis though their effect was not as yet detected because of methodical difficulties. Therefore, the problem of the antibiotic participation in regulation of sporulation in Bac. pumilus and Bac. subtilis remains open.

  16. Learners with Low Self-Efficacy for Information Literacy Rely on Library Resources Less Often But Are More Willing to Learn How to Use Them

    Directory of Open Access Journals (Sweden)

    Dominique Daniel

    2014-09-01

    Full Text Available Objectives – To determine whether there is a relationship between self-efficacy (i.e., confidence regarding information literacy skills and self-efficacy for distance learning; and to compare the use of electronic resources by high and low information literacy self-efficacy distance learners and their interest in learning more about searching. Design – Online survey. Setting – A small public university in the United States of America. Subjects – Undergraduate and graduate students enrolled in one or more online courses. Most respondents were in their twenties, 76% were female, 59% were undergraduates, and 69% were full time students. Methods – Students were asked six demographic questions, eight questions measuring their self-efficacy for information literacy, and four questions measuring their self-efficacy for online learning. All self-efficacy questions were adapted from previous studies and used a one to five Likert scale. The response rate was 6.2%. Correlational analysis was conducted to test the first two hypotheses (students who have higher self-efficacy for information seeking are more likely to have higher self-efficacy for online learning and for information manipulation. Descriptive analysis was used for the remaining hypotheses, to test whether students who have higher information literacy self-efficacy are more likely to have high library skills (hypothesis three and are more interested in learning about how to use library resources (hypothesis four. Among respondents high information literacy self-efficacy and low self-efficacy groups were distinguished, using the mean score of information literacy self-efficacy. Main Results – There was a significant correlation between self-efficacy for information seeking and self-efficacy for online learning (r = .27, as well as self-efficacy for information manipulation (r = .79. Students with high information seeking self-efficacy were more likely to use library databases (28

  17. Comparative analysis of a BAC contig of the porcine RN region and the human transcript map: implications for the cloning of trait loci.

    Science.gov (United States)

    Jeon, J T; Amarger, V; Rogel-Gaillard, C; Robic, A; Bongcam-Rudloff, E; Paul, S; Looft, C; Milan, D; Chardon, P; Andersson, L

    2001-03-15

    The poorly developed transcript maps and the limited resources for genome analysis hamper positional cloning of trait loci in farm animals. This study demonstrates that this will now be easier by the combined use of BAC contigs and the import of the near complete human transcript map. The conclusion was obtained by a comparative analysis of a 2.4-Mb BAC contig of the RN region in pigs. The contig was constructed as part of a successful positional cloning project, which identified PRKAG3 as the causative gene for the RN phenotype. A comparative map including the corresponding regions on human chromosome 2q35 and mouse chromosome 1 (region 36-44 cM) is reported. Sixteen coding sequences were mapped on the BAC contig. The majority of these were identified by BLAST searches of BAC end sequences and BAC shotgun sequences generated during the positional cloning project. Map data for the orthologues in humans were available for 12 of the 16 coding sequences, and all 12 have been assigned to 2q35. Furthermore, no evidence for any rearrangement in gene order was obtained. The extensive linkage conservation indicates that the near complete human transcript map will be an invaluable resource for positional cloning projects in pigs and other domestic animals.

  18. High-resolution chromosome mapping of BACs using multi-colour FISH and pooled-BAC FISH as a backbone for sequencing tomato chromosome 6

    NARCIS (Netherlands)

    Szinay, D.; Chang, S.B.; Khrustaleva, L.I.; Peters, S.A.; Schijlen, E.G.W.M.; Bai, Y.; Stiekema, W.; Ham, van R.C.H.J.; Jong, de H.; Klein Lankhorst, R.M.

    2008-01-01

    Within the framework of the International Solanaceae Genome Project, the genome of tomato (Solanum lycopersicum) is currently being sequenced. We follow a 'BAC-by-BAC' approach that aims to deliver high-quality sequences of the euchromatin part of the tomato genome. BACs are selected from various li

  19. 图情资源发现系统的研究与实现*%Research and Implementation of Libraries' Resource Discovery System

    Institute of Scientific and Technical Information of China (English)

    王灏; 张正锋; 冯巍

    2013-01-01

      资源发现系统是一种面向图书情报领域的垂直搜索引擎。文章从图情机构馆藏和服务的问题入手,介绍了资源发现系统的概念和技术路线,并以“中国学术搜索网”为例,介绍了该平台在资源发现服务中的总体设计、关键技术、核心功能和服务方式,旨在帮助图书馆等单位在产品技术选型和建设过程中提供可能的参考依据。%Resource discovery system is a kind of vertical search engine focused on library and information science. For the general problem in libraries and information centers, this article gave a brief introduction of the concept and main technologies. With the example of"Chinese Academic Search,"it has provided some practical details about the design, main function and service model of resource systems in order to provide suggestions for others' purchase or construction of discovery service if possible.

  20. L’italiano alla prova del Bac

    Directory of Open Access Journals (Sweden)

    Giulia Massignan

    2013-07-01

    Full Text Available Il lavoro propone alcune attività didattiche di preparazione dell’esame orale di italiano previsto dal Baccalauréat del liceo francese. Tale prova d’esame è stata recentemente oggetto di una riforma ministeriale volta a dare maggior peso alla pratica orale. Dopo uno studio delle nuove modalità della prove d’esame e dei suoi contenuti, ci si concentrerà sull’analisi delle abilità orali necessarie al suo superamento e sugli elementi linguistici e comunicativi su cui porre l’attenzione. Sulla base di queste premesse teoriche e metodologiche, si presentano alcuni interventi didattici di preparazione all’esame: due attività per la prova di comprensione orale e due per la prova di produzione e interazione orale; in entrambi i casi, si insiste sull’importanza di proporre strategie d’ascolto e di parlato insegnabili, che diventino consuetudini attive e parte integrante del bagaglio comunicativo del discente. The italian exam in the FrenchBacThe paper proposes some educational activities to prepare for the oral Italian examination as part of the French Baccalauréat. This exam has recently been the subject of a ministerial reform aimed at giving more weight to the oral practice. After illustrating new examination methods and content, we will focus on the analysis of oral skills necessary for passing the exam and aspects of language and communication on which to focus. Based on these theoretical and methodological premises, some educational exam preparation activities will be presented. Two activities test listening comprehension and two focus on production and oral interaction. The stress is on the importance of proposing teach able listening and speaking strategies, so that they become active habits and integral parts of the communicative learner’s baggage.

  1. Share and share alike: encouraging the reuse of academic resources through the Scottish electronic Staff Development Library

    Directory of Open Access Journals (Sweden)

    Lorna M. Campbell

    2001-12-01

    Full Text Available The Scottish electronic Staff Development Library (http://www.sesdl.scotcit.acuk is an ongoing collaborative project involving the Universities of Edinburgh, Paisley and Strathclyde which has been funded by SHEFC as part of their current ScotCIT Programme (http:llwww.scotcit.ac.uk. This project is being developed in response to the increasing demand for flexible, high-quality staff development materials.

  2. Kerala Public Library Network: A Project Report

    OpenAIRE

    Jayakrishnan, J S

    2013-01-01

    Public library systems in India are not so effective due to lack of efficient resource sharing and networking to achieve optimum utility of resources as well as to provide need based services. The present library system can be improved by application of ICT and enabling participation. A Kerala Public Library Network (KPLNET), and use of an integrated library automation package in all public libraries can achieve this. The web portal of the Public Library Network maintained by Kerala State Lib...

  3. The first generation of a BAC-based physical map of Brassica rapa

    Directory of Open Access Journals (Sweden)

    Lee Soo

    2008-06-01

    Full Text Available Abstract Background The genus Brassica includes the most extensively cultivated vegetable crops worldwide. Investigation of the Brassica genome presents excellent challenges to study plant genome evolution and divergence of gene function associated with polyploidy and genome hybridization. A physical map of the B. rapa genome is a fundamental tool for analysis of Brassica "A" genome structure. Integration of a physical map with an existing genetic map by linking genetic markers and BAC clones in the sequencing pipeline provides a crucial resource for the ongoing genome sequencing effort and assembly of whole genome sequences. Results A genome-wide physical map of the B. rapa genome was constructed by the capillary electrophoresis-based fingerprinting of 67,468 Bacterial Artificial Chromosome (BAC clones using the five restriction enzyme SNaPshot technique. The clones were assembled into contigs by means of FPC v8.5.3. After contig validation and manual editing, the resulting contig assembly consists of 1,428 contigs and is estimated to span 717 Mb in physical length. This map provides 242 anchored contigs on 10 linkage groups to be served as seed points from which to continue bidirectional chromosome extension for genome sequencing. Conclusion The map reported here is the first physical map for Brassica "A" genome based on the High Information Content Fingerprinting (HICF technique. This physical map will serve as a fundamental genomic resource for accelerating genome sequencing, assembly of BAC sequences, and comparative genomics between Brassica genomes. The current build of the B. rapa physical map is available at the B. rapa Genome Project website for the user community.

  4. Library/vendor relationships

    CERN Document Server

    Brooks, Sam

    2014-01-01

    A view of the mutual dependence between libraries and vendorsAs technology advances, libraries are forced to reach beyond their own resources to find effective ways to maintain accuracy and superior service levels. Vendors provide databases and integrated library systems that perform those functions for profit. Library/Vendor Relationships examines the increasing cooperation in which libraries find they must participate in, and vice versa, with the vendors that provide system infrastructure and software. Expert contributors provide insights from all sides of this unique collaboration, offering

  5. 蚊虫乙酰胆碱酯酶的真核表达、纯化及活性测定%Expression and purification of acetylcholinesterase from culex by Bac-to-Bac baculovirus system and the activity determination

    Institute of Scientific and Technical Information of China (English)

    谭烽; 兰文升; 崔峰; 乔传令; 陈雯莉

    2012-01-01

    利用Trizol法从尖音库蚊中提取总RNA,构建cDNA文库,并克隆出乙酰胆碱酯酶外显子序列;利用Bac-to-Bac杆状病毒表达系统对蚊虫乙酰胆碱酯酶进行真核表达,并利用Ni-琼脂糖对酶进行纯化.采用SDS-PAGE对纯化产物进行检测,结果表明得到了纯度较高的乙酰胆碱酯酶.参照Ellman法对酶的活性进行测定,结果显示纯酶的活力为2.219×10-4mol/(min·g).%The residues of the high toxic organophosphorous can cause chronic poisoning. At present, the detection of organophosphorous residue needs complicated operation, time consuming and high cost. Here,we harvested total RNA of mosquito by Trizol methods, constructed cDNA library, and got the exon of acetylcholinesterase. The acetylcholinesterase from culex was expressed by Bac-to-Bac system, then the enzyme was purified using Ni-sepharose. The crude and purified enzyme was tested and verified by SDS-PAGE. The enzyme activity was determined by Ellman methods. It will be of great significance to biosensors in organophosphorous pesticides detection.

  6. 开放获取对图书馆信息资源建设的影响%Impact of Open Access on Information Resources Construction in Library

    Institute of Scientific and Technical Information of China (English)

    麻思蓓

    2015-01-01

    介绍了国内外开放获取最新进展。通过对比分析开放获取资源和印刷型期刊及数据库的特点和优劣势,阐述了开放获取对图书馆信息资源建设战略、馆藏结构、组织揭示和共建共享所带来的影响。从我国情况看,今后一段时间,开放资源、数字化印本资源、数据库将同时作为馆藏共存,亟需探索和研究适合远程数字型网络资源的组织规范,信息资源的开放和共享是大势所趋。%Introduced latest progress on open access at home and abroad. By comparing and analyzing the advantages and disadvantages of OA resources , printing periodicals and databases, this paper expounds the profound impact of OA on information resource construction, collection structure, resource description and knowledge sharing in library. According to the speciifcs in China, open resources will coexist with digital print documents and databases in the next period of time. It’s urgent need to explore and research more suitable cataloging rules for internet digital resources. Openning and sharing of information resources is coming trend.

  7. Analysis of Optimization on Paper Resources and Digital Resources in Library%图书馆纸质资源与电子资源优化配置分析

    Institute of Scientific and Technical Information of China (English)

    尹爱兰

    2012-01-01

    This text fast summaries budget line, indifference curves and utility maximization of economy, and whose are explained in library; then analyzes the optimization of paper resources and digital resources of fixed expenses in library, finally, lists the corresponding function of the type and comes up with a way to find the optimal solution.%在预算线、无差异曲线及效用最大化等相关经济学理论知识的基础上对图书馆采购预算线、读者无差异曲线及读者效用最大化进行了诠释,接着从读者对信息需求的角度出发,对图书馆在有限采购经费的前提下如何优化配置纸质资源和电子资源进行了详细的分析,最后再列出相应函数关系式并得出一种求最优解的方法。

  8. The Thinking of Print Resource Development in a University Library in the Digital Age%数字化时代高校图书馆纸质馆藏资源建设的思考

    Institute of Scientific and Technical Information of China (English)

    彭少杰; 岳国庆

    2012-01-01

    图书馆网络化、数字化的发展,给馆藏结构及服务模式带来了深刻的变革。对数字化时代高校图书馆纸质馆藏资源建设进行了重新思考,重点阐述了新形势下馆藏结构的优化、学科分布、专业特色、开发利用以及纸质文献的共建共享等问题。%The development of interact and digital resources in libraries brings the revolutionary changes of the library collection structures and library services. The author of this article rethinks the establishment of print resources in a university library in the digital age. It introduces the issue of the optimization in the library collection structure, subject distribution, the specialties of the library, and the utilization and communal building and sharing of print resources in this new trend.

  9. Spectral library searching in proteomics.

    Science.gov (United States)

    Griss, Johannes

    2016-03-01

    Spectral library searching has become a mature method to identify tandem mass spectra in proteomics data analysis. This review provides a comprehensive overview of available spectral library search engines and highlights their distinct features. Additionally, resources providing spectral libraries are summarized and tools presented that extend experimental spectral libraries by simulating spectra. Finally, spectrum clustering algorithms are discussed that utilize the same spectrum-to-spectrum matching algorithms as spectral library search engines and allow novel methods to analyse proteomics data.

  10. Completely Distinguishing Individual A-genome Chromosomes and Their Karyotyping Analysis by Multiple BAC-FISH

    Institute of Scientific and Technical Information of China (English)

    WANG Kai; GUO Wang-zhen; ZHANG Tian-zhen

    2008-01-01

    @@ Multiple BAC-FISH is a powerful tool for modern cytogenetic researching in both animals and plants.But in cotton,this technique is unavailable due to the high percentage of repetitive sequences.Here,we identified twenty BACs from more than fifty BACs,and successfully demonstrated the use of multiple BAC-FISH for cytogenetie research in a diploid cotton species,G.arboreum.The karyotyping should be a basic application of this technique,but the potential usage such as high-resolution physical mapping construction,assisting BAC-by-BAC sequencing will be invaluable.

  11. Research on digital teaching resource library mode in distance education based on cloud computing%基于云计算的远程教育数字化教学资源库模式研究

    Institute of Scientific and Technical Information of China (English)

    刘晓丹; 蒋漪涟

    2015-01-01

    In order to realize the sharing of learning resources in the distance education and meet the needs of dif -ferent learners , it analyzes the digital teaching resource library in distance education from the cloud computing technology , proposes the teaching resource library model and the key technology , discusses the construction of teaching resource library mode , establishes the logical structure and platform structure of resource library mode , points out the broad prospect of the application of cloud computing technology in the construction of distance edu -cation resources .%为实现远程教育中的学习资源共享,满足不同学习者对资源的需求,对远程教育数字化教学资源库模式进行了研究. 从云计算技术、教学资源库模式、关键技术3个方面探讨了教学资源库的模式建设,阐述了资源库模式的逻辑架构和平台架构,指出了云计算技术在远程教育资源库建设中应用的广阔前景.

  12. Collaborative Portfolio's Effect on Library Usage

    Science.gov (United States)

    Bryan, Valerie

    2011-01-01

    Library resources are expensive and it is the library media specialist's responsibility to ensure that use of the library's resources is maximized to support the School Strategic Plan (SSP). This library usage study examined data on the scheduling of high school classes for research-based assignments, related to content area curriculum standards,…

  13. Digital Stories Resources Construction in Australian Libraries and Its Enlightenment for China%澳大利亚图书馆数字故事资源建设及其对我国的启示

    Institute of Scientific and Technical Information of China (English)

    黄文冰

    2015-01-01

    This paper introduces digital stories resources construction carried out in the public libraries and school libraries of Australia, analyzes the significance of carrying out digital stories resources construction in our country's libraries, and puts forward some measures for carrying out digital stories resources construction.%介绍了澳大利亚公共图书馆与学校图书馆开展的数字故事资源建设,阐述了在我国图书馆开展数字故事资源建设的意义,提出了开展数字故事资源建设的若干举措.

  14. Genomic libraries: I. Construction and screening of fosmid genomic libraries.

    Science.gov (United States)

    Quail, Mike A; Matthews, Lucy; Sims, Sarah; Lloyd, Christine; Beasley, Helen; Baxter, Simon W

    2011-01-01

    Large insert genome libraries have been a core resource required to sequence genomes, analyze haplotypes, and aid gene discovery. While next generation sequencing technologies are revolutionizing the field of genomics, traditional genome libraries will still be required for accurate genome assembly. Their utility is also being extended to functional studies for understanding DNA regulatory elements. Here, we present a detailed method for constructing genomic fosmid libraries, testing for common contaminants, gridding the library to nylon membranes, then hybridizing the library membranes with a radiolabeled probe to identify corresponding genomic clones. While this chapter focuses on fosmid libraries, many of these steps can also be applied to bacterial artificial chromosome libraries.

  15. 基于内部云存储的图书馆数据资源存储研究%Design for Library Data Resources Storage System Based on Internal Cloud Storage

    Institute of Scientific and Technical Information of China (English)

    乔杨

    2012-01-01

    Reliable storage environment is the basis for library digital resources providing efficient services for users. Based on the discussion of library digital resources storage different technologies, cloud storage connotation and applications in library are analyzed in this paper. According to the characteristics of library digital resources, a storage architecture based on internal cloud storage for library digital resources is designed. And the hierarchical structure, the methods of access control, expansion and deployment are detailed.%可靠存储环境是图书馆数字资源为用户提供高效服务的保障。在阐述图书馆数据资源存储技术基础上,分析了云存储内涵及在图书馆中的应用,探讨基于内部云存储的图书馆数字资源数据存储方案,详细分析了其层次架构、访问控制、扩展和部署方法。

  16. Chromosomal localization of heat shock protein 70 (HSP70) gene in Zhikong scallop Chlamys farreri using BAC-FISH%栉孔扇贝热休克蛋白70(HSP70)基因的BAC-FISH定位

    Institute of Scientific and Technical Information of China (English)

    郇聘; 张晓军; 李富花; 张洋; 赵翠; 刘保忠; 相建海

    2009-01-01

    Zhikong scallop (Chlamys farreri) is one of key species in the marine aquaculture of China. Till present, the disease control is the main issue and many subjects were focused on disease- resistance and stress-related genes. Heat shock protein 70 (HSP70) shows diverse functions in the anti-stress reaction and pathogen-resistance reaction. However, the studies on HSP70 in C. farreri were mainly on a sequence analysis and expression profile, there is no result about the gene localization of HSP70. In this study, with the support of a BAC DNA library of C. farreri, a HSP70-eontaining BAC clone, CFB 123C08, was mapped to the long arms of a pair of homologous chromosomes of C. farreri using BAC-FISH. This study would facilitate further studies on the HSP70 of C. farreri. And as the first attempt of chromosomal mapping of low-copy genes in C. farreri, this study would promote the researches on the chromosome identification of C farreri.%利用BAC-FISH技术,将包含HSP70基因的BAC克隆定位到栉孔扇贝的一对同源染色体的长臂上.HSP70基因的染色体定位将对深入研究该基因的结构及功能并将其应用于生产实践提供基础支持.同时,本研究是首次对栉孔扇贝低拷贝基因进行染色体定位的实践,其结果将为栉孔扇贝的染色体的深入研究以及染色体鉴别等工作提供必要的参考.

  17. Research on the Development of Exhibition Service in Public Library from the Perspective of Resource Sharing%从资源共享角度看公共图书馆展览服务发展

    Institute of Scientific and Technical Information of China (English)

    杨秀齐

    2012-01-01

    简述公共图书馆展览服务发展现状,分析公共图书馆展览资源共享的意义,从资源共享的角度探讨了公共图书馆展览服务的发展路径。%This paper briefly narrates the development status of exhibition services in public library, analyses the signifi- cance for realizing exhibition resource sharing, and discusses the development path of the exhibition service in public library from the perspective of resource sharing.

  18. Probe into the Strategies for the Construction of Digital Resources in the Library of Higher Vocational College%高职院校图书馆数字化资源构建策略探讨

    Institute of Scientific and Technical Information of China (English)

    朱勇

    2014-01-01

    分析了高职院校图书馆数字化资源构建过程中存在的问题,提出了高职院校图书馆数字化资源构建策略。%ABSTRACT:This paper analyzes some problems existing in the strategies for the construction of digital resources in the library of higher vocational college, and puts forward some strategies for the strategies for the construction of digital resources in the library of higher vocational college.

  19. On the Application of Flexible Management in Human Resource Management of Library%柔性管理在图书馆人力资源管理中的应用研究

    Institute of Scientific and Technical Information of China (English)

    袁峰; 叶丽红

    2012-01-01

    在简述柔性管理涵义的基础上,针对图书馆人力资源管理现状,提出图书馆在人力资源管理中应用柔性管理的策略。%Based on the description of the meaning of flexible management,this paper puts forward the strategies of the application of flexible management in library human resource management according to the current situation of library human resource management.

  20. 论新信息环境下中小型图书馆信息资源建设%Discussion on Information Resources Construction of Small and Medium-sized Libraries in the New Information Environment

    Institute of Scientific and Technical Information of China (English)

    叶菁

    2015-01-01

    论述了新信息环境下中小型图书馆信息资源建设的定位,探讨了新信息环境下中小型图书馆信息资源建设的有效对策.%This paper discusses the positioning of information resources construction of small and medium-sized libraries in the new information environment, and probes into some effective countermeasures for small and medium-sized libraries to carry out the information resources construction in new information environment.

  1. LINX: managing Earth Sciences free web resources at Milano Bicocca University Library = LINX: la gestione delle risorse web di Scienze della Terra della biblioteca di Ateneo di Milano-Bicocca

    OpenAIRE

    Papalia, Chiara; Saccenti, Luisanna; Verga, Francesca

    2007-01-01

    The library of the Università degli Studi Milano-Bicocca has adopted the Scout Portal Toolkit (SPT) open-source software to catalog, display, and share collections of data about web resources. The result was the creation of “LINX – Link e strumenti X la ricerca”, which has a dedicated Earth Sciences section. LINX is a collection of free resources for education and research selected by the library staff and/or recommended by users: web sites, databases, virtual reference desks, repositories, e...

  2. Analysis on the Regional Cooperative Development of Digital Resources of Public Libraries---Taking Chuangchun Library as an Example%公共图书馆数字资源的区域合作发展探析--以长春图书馆为例

    Institute of Scientific and Technical Information of China (English)

    岳晓波

    2014-01-01

    针对多数图书馆在数字资源建设方面存在的中外文资源配置不均衡、资源重复配置现象严重等问题,以长春图书馆为例,探讨了公共图书馆数字资源建设应采取的措施,以促进区域内图书馆数字资源的协调发展与合作建设,实现数字资源的合理配置与有效利用。%In the light of some problems existing in digital resources construction of most of the libraries such as the imbalanced allocation of Chinese-foreign resources, and serious repetition of resources allocation, etc., and taking Chuangchun Library as an example, this paper probes into the measures that the public library should take in digital resources construction for promoting the coordinated development and cooperative construction of digital resources of regional libraries and realizing the reasonable configuration and effective use of digital resources.

  3. An overview of the Phalaenopsis orchid genome through BAC end sequence analysis

    Directory of Open Access Journals (Sweden)

    Hsiao Yu-Yun

    2011-01-01

    Full Text Available Abstract Background Phalaenopsis orchids are popular floral crops, and development of new cultivars is economically important to floricultural industries worldwide. Analysis of orchid genes could facilitate orchid improvement. Bacterial artificial chromosome (BAC end sequences (BESs can provide the first glimpses into the sequence composition of a novel genome and can yield molecular markers for use in genetic mapping and breeding. Results We used two BAC libraries (constructed using the BamHI and HindIII restriction enzymes of Phalaenopsis equestris to generate pair-end sequences from 2,920 BAC clones (71.4% and 28.6% from the BamHI and HindIII libraries, respectively, at a success rate of 95.7%. A total of 5,535 BESs were generated, representing 4.5 Mb, or about 0.3% of the Phalaenopsis genome. The trimmed sequences ranged from 123 to 1,397 base pairs (bp in size, with an average edited read length of 821 bp. When these BESs were subjected to sequence homology searches, it was found that 641 (11.6% were predicted to represent protein-encoding regions, whereas 1,272 (23.0% contained repetitive DNA. Most of the repetitive DNA sequences were gypsy- and copia-like retrotransposons (41.9% and 12.8%, respectively, whereas only 10.8% were DNA transposons. Further, 950 potential simple sequence repeats (SSRs were discovered. Dinucleotides were the most abundant repeat motifs; AT/TA dimer repeats were the most frequent SSRs, representing 253 (26.6% of all identified SSRs. Microsynteny analysis revealed that more BESs mapped to the whole-genome sequences of poplar than to those of grape or Arabidopsis, and even fewer mapped to the rice genome. This work will facilitate analysis of the Phalaenopsis genome, and will help clarify similarities and differences in genome composition between orchids and other plant species. Conclusion Using BES analysis, we obtained an overview of the Phalaenopsis genome in terms of gene abundance, the presence of repetitive

  4. A Preliminary Discussion on Information Resources Integration in Hospital Libraries%医院图书馆信息资源整合初探

    Institute of Scientific and Technical Information of China (English)

    唐莉

    2012-01-01

    分析医院图书馆在医院信息化建设中面临的功能萎缩、人员整体素质不高及孤立分离3个方面的困境,指出全面整合信息资源是提高医院医疗质量、服务水平及管理决策的有效途径,探讨医院图书馆在信息资源整合中的发展方向。%The paper analyzes the library problems existed in hospital informatization construction process,including functional atrophy, deficiency of staff's overall quality,isolation separation,etc.It points out that information resources comprehensive integration is an effective way to improve medical care quality,service level and management decision in hospitals,discusses the development direction of hospital library in information it sources integration.

  5. Thinking on Constriction of Literature Resource System in Libraries%对图书馆文献资源体系建设的思考

    Institute of Scientific and Technical Information of China (English)

    黄芳

    2009-01-01

    对图书馆馆藏文献资源构成进行合理分类,同时对其特点进行科学分析,提出调整馆藏发展政策、建立特色化馆藏体系和优化采访人员素质等3大措施来优化图书馆文献资源体系建设.%Carrying out reasonable classification of literature collection and analyzing the characteristics scientifically, the paper points out adjusting development policies for collection, constructing characteristic collection system and optimizing the quality of acquisition librarian, which would play an positive role in improving the construetion of literature resource system in libraries.

  6. A High Efficient Approach Used for BAC-contig Extension of Oryza sativa with PCR Screening the BAC Clone Pools%一种使用混合PCR筛选技术高效延伸水稻BAC-重叠群的方法

    Institute of Scientific and Technical Information of China (English)

    胡昊; 李滔; 穆洁; 韩斌; 洪国藩

    2002-01-01

    使用"克隆连克隆(clone by clone)" 战略进行水稻基因组测序需要依赖于构建好的基因组物理图. 工作着眼于水稻籼稻广陆矮4号(Oryza sativa indica GuangLuAi4)第四号染色体长臂上56.1~68 cM的区域, 采用PCR方法筛选BAC全库来延伸重叠群, 构建物理图. 通过参照特异遗传探针定位的BAC克隆(seed BAC)末端序列设计了14对引物, 按特定规则分成3组, 分别以代表水稻BAC 库( 共22 368个BAC )的233个BAC pool为模板进行PCR反应, 一共获得了65个阳性BAC克隆, 通过末端测序、酶切杂交等方法确定了其中29个BAC克隆作为有效延伸的克隆, 延伸了8个重叠群. 通过酶切杂交、末端测序等方法还获知阳性BAC的延伸方向、延伸长度以及与seed BAC之间的重叠长度. 8个重叠群总的延伸长度达到510 kb. 与实验室原用于作物理图的其他方法如指纹图、点杂交等相比, 该方法有高效率、高灵敏度、专一性好、可重复使用等优点. 创新之处在于通过引物的合理分组和PCR实验条件的改进降低了假阳性和假阴性率.%To extend 8 BAC contigs, which were previously located in the 56.1—68 Cm region of the chromosome 4 of the Oryza sativa indica GuangLuAi4, 14 pairs of primers were designed according to the terminal sequences of the existing seed BACs and were deliberately divided into 3 groups. With the 3 groups of primer mixtures, 233 pools of BAC DNA that represent 22 368 BAC clones from O.sativa indica GuangLuAi4 genomic library were screened. 65 positive clones corresponding to the 8 contigs were isolated and 29 clones of them were confirmed to be extended to the seed BACs by end sequencing and fingerprinting. The protocol greatly enhanced the efficiency of the contig extension and was also superior for its specificity, sensitivity and reusability to the colony in situ hybridization which is a conventional method employed in contig extension and physical map construction.

  7. Research on Colleges and Universities’Serving Local Cultural Development by Their Library Resources---Taking Anhui Science and Technology University Library as an Example%高校馆藏资源服务地方文化建设管窥--以安徽科技学院图书馆为例

    Institute of Scientific and Technical Information of China (English)

    王友莲; 卫胜

    2014-01-01

    高校馆藏资源提供利用是建设地方文化的重要保障条件之一。高校图书馆在弘扬和促进地方文化建设方面要充分发挥丰富的馆藏资源优势并定位好服务角色。2006年起安徽科技学院图书馆主动对接地方文化建设,面向社会读者陆续开放其馆藏资源。总结安徽科技学院馆藏资源服务地方文化建设的经验如下:转变思想,树立服务社会的理念;利用馆藏资源主动对接地方文化发展的需要;面向社会开放馆藏资源;实现了高校馆藏资源与地方文化建设的良性循环与互动。%The availability of library resources of colleges and universities is one of the important guarantees of local cultural development. Their libraries should give full play to the advantages of their rich library resources and determine their service role in the promotion of local cultural development. Since 2006, the library of Anhui Science and Technology University has been volunteering to connect itself with local cultural development and making its library resources available for social readers. Through the author summarized experience of it universities in their serving local cultural development with library resources, hoping to provide reference for relevant practices and studie:Change their thinking, carding community service concept, Use library resources to connect with the needs of local cultural development. Open society library resources; Achieve a virtuous cycle of interaction with the local university library resources and cultural construction.

  8. Enhancing Digital Information Access in Public Libraries

    OpenAIRE

    Ayers, Kathleen; Liu, Yan Quan

    2006-01-01

    To explore the impact of public digital library resources on urban residents, to elucidate the current usage patterns of public digital library resources/services and levels of satisfaction with the resources/content in urban digital libraries and make recommendations as to steps that would improve service to this population.

  9. DLESE Teaching Box Pilot Project: Developing a Replicable Model for Collaboratively Creating Innovative Instructional Sequences Using Exemplary Resources in the Digital Library for Earth System Education (DLESE)

    Science.gov (United States)

    Weingroff, M.

    2004-12-01

    Before the advent of digital libraries, it was difficult for teachers to find suitable high-quality resources to use in their teaching. Digital libraries such as DLESE have eased the task by making high quality resources more easily accessible and providing search mechanisms that allow teachers to 'fine tune' the criteria over which they search. Searches tend to return lists of resources with some contextualizing information. However, teachers who are teaching 'out of discipline' or who have minimal training in science often need additional support to know how to use and sequence them. The Teaching Box Pilot Project was developed to address these concerns, bringing together educators, scientists, and instructional designers in a partnership to build an online framework to fully support innovative units of instruction about the Earth system. Each box integrates DLESE resources and activities, teaching tips, standards, concepts, teaching outcomes, reviews, and assessment information. Online templates and best practice guidelines are being developed that will enable teachers to create their own boxes or customize existing ones. Two boxes have been developed so far, one on weather for high school students, and one on the evidence for plate tectonics for middle schoolers. The project has met with significant enthusiasm and interest, and we hope to expand it by involving individual teachers, school systems, pre-service programs, and universities in the development and use of teaching boxes. A key ingredient in the project's success has been the close collaboration between the partners, each of whom has brought unique experiences, perspectives, knowledge, and skills to the project. This first effort involved teachers in the San Francisco Bay area, the University of California Museum of Paleontology, San Francisco State University, U.S. Geological Survey, and DLESE. This poster will allow participants to explore one of the teaching boxes. We will discuss how the boxes were

  10. Academic Libraries in Japan

    Science.gov (United States)

    Cullen, Rowena; Nagata, Haruki

    2008-01-01

    Academic libraries in Japan are well resourced by international standards, and support Japan's internationally recognized research capability well, but there are also ways in which they reflect Japan's strong bureaucratic culture. Recent changes to the status of national university libraries have seen a new interest in customer service, and…

  11. Services and Resources to Engineers: A Case Study of Outreach and Marketing, Assessment, and Future Directions in a Research Library

    Science.gov (United States)

    Torrence, Matt

    2015-01-01

    The literature, activities, and resource needs of engineering students and faculty provide insight into a demographic that is often among the early-adopters of new technologies, tools, and methods of sharing information. Despite the often non-bibliographic nature of their research efforts, there are numerous elements of the traditional service…

  12. Genomic resources for water yam (Dioscorea alata L.): analyses of EST-Sequences, De Novo sequencing and GBS libraries

    Science.gov (United States)

    The reducing cost and rapid progress in next-generation sequencing techniques coupled with high performance computational approaches have resulted in large-scale discovery of advanced genomic resources such as SSRs, SNPs and InDels in several model and non-model plant species. Yam (Dioscorea spp.) i...

  13. News from the Library

    CERN Multimedia

    CERN Library

    2010-01-01

    The LHC Library to be merged with the Central Library. Not everyone knows that CERN Scientific Information Service currently counts three physical libraries on site. The Central Library is located in Building 52 and there are two satellite libraries located respectively in building 30 (the LHC Library) and in building 864 on Prévessin site (the SPS Library). Moreover, the Legal Service Library is located in Building 60. In the past, there have been at CERN up to 6 satellite libraries; they were essential at a time when information was only in paper form and having multiple copies of documents located in several places at CERN was useful to facilitate scientific research. Today, this need is less critical as most of our resources are online. That is why, following a SIPB (Scientific Information Policy Board) decision, the collections of the LHC Library will be merged this summer with the Central collection. This reorganization and centralization of resources will improve loan services. The SP...

  14. Iowa DNR - NRGIS Library

    Data.gov (United States)

    Iowa State University GIS Support and Research Facility — The Natural Resources Geographic Information System (NRGIS) Library is a Geographic Information System (GIS) repository developed and maintained by the GIS Section...

  15. A Study of Ontology Model of Resource Organization and Management of Library Service%图书馆服务性资源组织与管理的本体模型研究

    Institute of Scientific and Technical Information of China (English)

    张晗; 孙翌; 郑巧英

    2016-01-01

    文章通过调研国内外高校图书馆及相关研究,对图书馆服务性资源进行定义与分类,在此基础上,利用本体技术设计图书馆服务性资源一体化组织与管理模型:以服务为主线整合并关联馆藏服务资源、人力资源、场所资源等服务性资源,对图书馆服务本体进行建模,给出其类、属性和关系的定义。%With an investigation on domestic and overseas university libraries and related researches, the definition and classification of library service resource are discussed. On that basis, an integrative resource organization and management model of library service is designed with ontology technology:organically integrating and associating all library service resources, such as collection, orkforce and places;modeling library service ontology by giving definitions to its classes,properties and relationship.

  16. 76 FR 44055 - BAC Home Loans Servicing, LP, et al.; Notice of Application and Temporary Order

    Science.gov (United States)

    2011-07-22

    ... COMMISSION BAC Home Loans Servicing, LP, et al.; Notice of Application and Temporary Order July 18, 2011... Act, with respect to an injunction entered against BAC Home Loans Servicing, LP (``HLS'') on May 31... (``BAC''). HLS is an entity that services mortgage loans and provides mortgage services,...

  17. Investigation into the Utilization Situation of Learning Resources of Digital Libraries%数字图书馆学习资源使用现状调查分析

    Institute of Scientific and Technical Information of China (English)

    柏菊花; 陶秋荣; 于素云; 徐明

    2011-01-01

    Investigation into the utilization situation of learning resources of digital libraries by master of educational technology in about 20 universities shows that there is no logic relationship between learning resources of digital libraries and utilization average; there are complicated hindering factor in the utilization of learning resources of digital libraries; the utilization rate of digital libraries is lower than the utilization rate of search engine; users are lack of awareness of resources contribution. Therefore, we should research the utilization situation of learning resources by users continually, in order to improve library services and help readers to use learning resources better.%通过对我国近20所高校教育技术学专业的硕士研究生利用数字图书馆学习资源的调查表明,数字图书馆学习资源的评价和使用均值之间没有必然联系影响用户使用数字图书馆学习资源的阻碍因素复杂;数字图书馆的使用率低于搜索引擎;用户缺乏资源贡献意识。因此,应继续研究用户利用学习资源的情况,从而改善自身服务,帮助读者更好地利用学习资源。

  18. INFLUENCE OF COLURIA GEOIDES (ROSACEAE BAC ON HUMORAL IMMUNE RESPONCE

    Directory of Open Access Journals (Sweden)

    S. V. Dutova

    2014-01-01

    Full Text Available The article presents the results of immunocorrecting properties of dry extract fractions of C. geoides (Rosaceae for experimental immunodeficiency. Since the BAC fractions action relinquishes to the action of integral extract, the isolation of active substances is not reasonable

  19. Dormitory libraries: libraries in dormitories

    Directory of Open Access Journals (Sweden)

    Peter Pavletič

    2004-01-01

    Full Text Available Dormitory libries are not justly treated in Slovenia. They have a double purpose: to develop student literacy, especially reading, critical and creative competence and, moreover, to provide students with opportunities for learning and active spending of free-time. This is made possible by means of a good collection of expertly arranged library material, which is regulary updated and presented to its users, both students and tutors alike. A questionnaire has helped us to find out that libraries in secondary school dormitories carry out their work rather successfully, especially from the viewpoint of poor facilities. The major problems are, nevertheless, the appropriate qualifications of those who fill the posts of librarian and low financial resources. Therefore, such activities should be thoroughly analysed and reconsidered in terms of possible effective solutions, if we want to at least maintain them, let alone develop them.

  20. Evaluation of Digital Library Resources Based on the Third Party Platform%基于第三方平台的数字图书馆资源评价研究

    Institute of Scientific and Technical Information of China (English)

    曹异卿; 唐俊

    2013-01-01

    随着互联网的发展,数字图书馆在现代图书馆中占据着越来越重要的地位.论文针对目前数字图书馆读者交流不便,数字资源的读者评价匮乏,不同数字图书馆之间的数字资源评价无法实现共享和馆际交流等问题,提出了一个基于第三方平台的数字图书馆资源评价模式.第三方平台采用Web服务的方式,根据客户提供的数字资源唯一识别信息为客户提供数字资源评价服务.通过在数字图书馆中引入第三方评价平台的模式,不仅可以解决图书馆数字资源评价的问题,而且实现了跨馆的数字资源评价交流.%With the development of internet,the digital library has played a more and more important role in the modern library.In this article,aiming to problems of the digital library including the poor communication with the readers,the lacking of the readers' comments and failing to share the digital resource evaluation and the exchanges between the digital libraries,we present a resource evaluation mode based on the third-party platform for the digital library.By means of web service and according to the digital resource's unique identifying information provided by the customers,the third-party platform will provide the customers with the digital resource evaluation service.With the intro duction of such mode of the third-party evaluation platform into the digital library,it will not only solve the problem of digital resource evaluation in the library,but also realize the digital resource evaluation and exchanges across the libraries.

  1. 关于提高公安院校图书馆数字资源利用率的探析%On the Improvement of Digital Resource Utilization Ratio of Police Universities' Libraries

    Institute of Scientific and Technical Information of China (English)

    信晓红

    2012-01-01

    Police universities need to develop digital resource in library, but the existing conditions make the construction of library digital resource difficult to develop to an extent. So libraries are short of unique resources and difficult to attract the existing reader groups, the utilization ratio is low. We should provide the service of developing readers, build digital resource database and offer digital resource service, so we can improve digital resource utilization ratio.%公安院校图书馆需要发展数字资源馆藏,但现有条件使得数字资源建设难以形成规模,造成馆藏中现有的数字资源缺乏独到的资源优势,难以吸引现有的用户群体,利用率较低,需要从读者培养服务、数字馆藏建设、数字资源服务手段等方面加以调整,以提高馆藏数字资源的利用率。

  2. Thoughts on the Construction of Database Resources System in Private University Library%民办高校图书馆数据库资源建设思考

    Institute of Scientific and Technical Information of China (English)

    文炜

    2015-01-01

    The current situation of electronic resources in 8 private university libraries is investigated though the libraries' webpage. The statistics shows that these libraries' electronic resources have been less used due to the lack of unified classification and integration mechanism. This paper points out that the private university libraries should architect their own resource system combined with the service of national information resource system to keep the users satisfied.%通过浏览图书馆主页对对陕西省教育厅公布的8所民办高校图书馆的数据库资源建设情况进行统计分析,结果显示民办高校图书馆存在购买数据库品种单一、资源分类缺乏统一标准和整合机制、资源使用效率低等问题,指出民办高校图书馆一方面应充分借助国家信息资源保障体系力量,另一方面要依靠自身科学构建资源体系,同时做好用户培训工作,满足用户的文献信息需求.

  3. 高校图书馆开展地方文献资源建设工作探析%Discussion on construction of local document resources in university libraries

    Institute of Scientific and Technical Information of China (English)

    朱晓

    2013-01-01

    地方文献是一种独特的文献资源,具有地域性、多样性,是高校图书馆服务社会发展、推进文化传承创新的重要文献保障。切实做好地方文献资源建设工作,可以提升高校图书馆的核心服务能力,实现高校图书馆可持续发展。%Local documents as a kind of unique document resources occupy a special position in the collection sys -tem in university libraries , which provide resource support to develop characteristic service and improve ability of information service .The regional characteristics and diversities of local document provide important document secu -rity through which university libraries serve the economic and social development and promote the cultural inherit -ance and innovation .University libraries'effective construction of document resources can promote their core service ability based on local characteristics of the document information resources , which will assist university libraries in sustainable development in the face of the challenges of informatization , automation, digitization, diversification in today's society .

  4. Evidence Based Research Activities, Interests and Opportunities Exist for Practitioners in all Library Sectors in the British Isles. A review of: McNicol, Sarah. “Is Research an Untapped Resource in the Library and Information Profession?” Journal of Librarianship and Information Science 36.3 (September 2004:119-26.

    Directory of Open Access Journals (Sweden)

    Julie McKenna

    2006-03-01

    technology (ICT was an area of planned future research in all libraries, as were user needs and user behaviour. The most frequently cited barriers to research activity across all sectors were lack of time and financial resources. Staff skills and the lack of focus on practical problems to solve were indicated as a barrier in health, public and academic libraries. Libraries reported a range of common uses for the research findings including: informing strategic and service planning; providing benchmarking data and measuring the effectiveness of services; identification of marketing and public relations opportunities; discovery of staff training needs; and use of the results to demonstrate the value of libraries to funding bodies. Conclusion – This study provides insight into practitioner‐focused areas of research interest and possible areas for future investigation. As the author reports in her conclusion, the survey results cannot be considered representative of the wider population. Since research interests often overlap, a sector wide or cross‐sectoral research approach should be considered to allow library staff to identify and resolve common problems. Wide dissemination of research results within the practitioner community would be of benefit to all. Greater communication between practitioner and information science communities is also encouraged, as these communities’ work is mutually beneficial.

  5. Novel SSR markers from BAC-end sequences, DArT arrays and a comprehensive genetic map with 1,291 marker loci for chickpea (Cicer arietinum L..

    Directory of Open Access Journals (Sweden)

    Mahendar Thudi

    Full Text Available Chickpea (Cicer arietinum L. is the third most important cool season food legume, cultivated in arid and semi-arid regions of the world. The goal of this study was to develop novel molecular markers such as microsatellite or simple sequence repeat (SSR markers from bacterial artificial chromosome (BAC-end sequences (BESs and diversity arrays technology (DArT markers, and to construct a high-density genetic map based on recombinant inbred line (RIL population ICC 4958 (C. arietinum×PI 489777 (C. reticulatum. A BAC-library comprising 55,680 clones was constructed and 46,270 BESs were generated. Mining of these BESs provided 6,845 SSRs, and primer pairs were designed for 1,344 SSRs. In parallel, DArT arrays with ca. 15,000 clones were developed, and 5,397 clones were found polymorphic among 94 genotypes tested. Screening of newly developed BES-SSR markers and DArT arrays on the parental genotypes of the RIL mapping population showed polymorphism with 253 BES-SSR markers and 675 DArT markers. Segregation data obtained for these polymorphic markers and 494 markers data compiled from published reports or collaborators were used for constructing the genetic map. As a result, a comprehensive genetic map comprising 1,291 markers on eight linkage groups (LGs spanning a total of 845.56 cM distance was developed (http://cmap.icrisat.ac.in/cmap/sm/cp/thudi/. The number of markers per linkage group ranged from 68 (LG 8 to 218 (LG 3 with an average inter-marker distance of 0.65 cM. While the developed resource of molecular markers will be useful for genetic diversity, genetic mapping and molecular breeding applications, the comprehensive genetic map with integrated BES-SSR markers will facilitate its anchoring to the physical map (under construction to accelerate map-based cloning of genes in chickpea and comparative genome evolution studies in legumes.

  6. Bacteriocin protein BacL1 of Enterococcus faecalis is a peptidoglycan D-isoglutamyl-L-lysine endopeptidase.

    Science.gov (United States)

    Kurushima, Jun; Hayashi, Ikue; Sugai, Motoyuki; Tomita, Haruyoshi

    2013-12-27

    Enterococcus faecalis strains are commensal bacteria in humans and other animals, and they are also the causative agent of opportunistic infectious diseases. Bacteriocin 41 (Bac41) is produced by certain E. faecalis clinical isolates, and it is active against other E. faecalis strains. Our genetic analyses demonstrated that the extracellular products of the bacL1 and bacA genes, which are encoded in the Bac41 operon, coordinately express the bacteriocin activity against E. faecalis. In this study, we investigated the molecular functions of the BacL1 and BacA proteins. Immunoblotting and N-terminal amino acid sequence analysis revealed that BacL1 and BacA are secreted without any processing. The coincidental treatment with the recombinant BacL1 and BacA showed complete bacteriocin activity against E. faecalis, but neither BacL1 nor BacA protein alone showed the bacteriocin activity. Interestingly, BacL1 alone demonstrated substantial degrading activity against the cell wall fraction of E. faecalis in the absence of BacA. Furthermore, MALDI-TOF MS analysis revealed that BacL1 has a peptidoglycan D-isoglutamyl-L-lysine endopeptidase activity via a NlpC/P60 homology domain. These results collectively suggest that BacL1 serves as a peptidoglycan hydrolase and, when BacA is present, results in the lysis of viable E. faecalis cells.

  7. A library-based bioinformatics services program*

    OpenAIRE

    2000-01-01

    Support for molecular biology researchers has been limited to traditional library resources and services in most academic health sciences libraries. The University of Washington Health Sciences Libraries have been providing specialized services to this user community since 1995. The library recruited a Ph.D. biologist to assess the molecular biological information needs of researchers and design strategies to enhance library resources and services. A survey of laboratory research groups ident...

  8. Genomic Resources for Water Yam (Dioscorea alata L.: Analyses of EST-Sequences, De Novo Sequencing and GBS Libraries.

    Directory of Open Access Journals (Sweden)

    Christopher A Saski

    Full Text Available The reducing cost and rapid progress in next-generation sequencing techniques coupled with high performance computational approaches have resulted in large-scale discovery of advanced genomic resources in several model and non-model plant species. Yam (Dioscorea spp. is a major food and cash crop in many countries but research efforts have been limited to understand the genetics and generate genomic information for the crop. The availability of a large number of genomic resources including genome-wide molecular markers will accelerate the breeding efforts and application of genomic selection in yams. In the present study, several methods including expressed sequence tags (EST-sequencing, de novo sequencing, and genotyping-by-sequencing (GBS profiles on two yam (Dioscorea alata L. genotypes (TDa 95/00328 and TDa 95-310 was performed to generate genomic resources for use in its improvement programs. This includes a comprehensive set of EST-SSRs, genomic SSRs, whole genome SNPs, and reduced representation SNPs. A total of 1,152 EST-SSRs were developed from >40,000 EST-sequences generated from the two genotypes. A set of 388 EST-SSRs were validated as polymorphic showing a polymorphism rate of 34% when tested on two diverse parents targeted for anthracnose disease. In addition, approximately 40X de novo whole genome sequence coverage was generated for each of the two genotypes, and a total of 18,584 and 15,952 genomic SSRs were identified for TDa 95/00328 and TDa 95-310, respectively. A custom made pipeline resulted in the selection of 573 genomic SSRs common across the two genotypes, of which only eight failed, 478 being polymorphic and 62 monomorphic indicating a polymorphic rate of 83.5%. Additionally, 288,505 high quality SNPs were also identified between these two genotypes. Genotyping by sequencing reads on these two genotypes also revealed 36,790 overlapping SNP positions that are distributed throughout the genome. Our efforts in using

  9. Analysis of Collection Resources Optimization of University Library Based on Information Aggregation%基于信息聚合技术的高校图书馆馆藏资源优化分析

    Institute of Scientific and Technical Information of China (English)

    马灿

    2016-01-01

    〔Abstract〕For personalized service problem of university library collection resources in China, this paper studies to improve the utilization ratio of university library collection resources optimization strategy in China at present. With its information aggregation of metadata and semantic mode, and making it more optimized. 200 questionnaires were obtained from sample survey of working condition before and after optimization, and analysis was based on the data of the questionnaire. The results indicate that utilization ratio of university library collection resources is much higher than before, and the aim of push of personalized knowledge is achieved, resulting in rising of library resources utilization ratio, and the results provide the scientiifc and technical basis for improvement of the library collection resources optimization in China.%针对我国高校图书馆馆藏资源的个性化服务难题,文章指出提高我国高校图书馆馆藏资源利用率的优化策略。结合元数据方式和语义方式对其进行信息聚合,使其更加优化,并对优化前后的使用状况,采用抽样调查的方法获取200份调查问卷,通过对问卷的数据进行数据分析研究,其结果为优化我国高校图书馆馆藏资源提供了充分的科学依据。

  10. 国内主要医药图书馆数字资源建设现状调查%Status quo of digital resources development in domestic medical and pharmaceutical libraries

    Institute of Scientific and Technical Information of China (English)

    任慧玲; 汪庆; 周琴

    2015-01-01

    选取中国高等院校医药图书馆协会8所图书馆作为调研对象,统计各图书馆数字资源的语种、类型及数量,分析发现上述图书馆数字资源建设已经取得一定成效,但是存在新型数字资源建设有待加强、免费及开放资源整合不够、资源组织揭示不够深入等问题,并对此提出了优化数字资源结构、建设特色数字资源、深入组织揭示数字资源等对策。%The languages, types and number of digital resources in 8 domestic medical and pharmaceutical college and university libraries were analyzed , which showed that although certain successes have been achieved in devel-opment of digital resources in these libraries, there are some problems needing to be solved, such as weakness in new digital resources development , insufficient integration of free and open access resources , insufficient organiza-tion and revealing of resources .Suggestions were put forward for the solution of these problems , such as optimizing digital resource structure, developing characteristic digital resources, sufficient organizing and revealing digital re-sources.

  11. BEA数字图书馆:洞悉美国经济的窗口%BEA Digital Library, a Path to America's Economic Resources

    Institute of Scientific and Technical Information of China (English)

    刘燕权; 南文秀

    2012-01-01

    Lunched in 2006, the BEA digital library provides "the most timely, relevant, and accurate economic accounts data in an objective and cost-effective manner" free for public to access. Its collection pulls together primarily historic data and articles on the U.S. economic accounts from the Department of Commerce, Bureau of the Census and other US government divisions, allows users to explore the economic history of the United States along with the national income and product accounts and general macroceonomic situation. While this paper indicates its well-organized rich resources, technological and services features, author's comments and suggestion for the library's improvements are also given.%BEA (Bureau of Economy Analysis,美国国家经济分析局)在线图书馆建立于2006年,服务于美国从事经济数据统计工作的政府机构,同时面向社会公众提供免费的包括宏观和微观层面几乎所有经济活动的指标数据.其主要目的是发布美国历史及当前最新经济统计资料和评估调查报告,为不同的用户群体了解、研究美国经济提供良好的服务平台,包括搜索、浏览及下载服务.其资源涵盖范围广泛,有很高的科研及实际参考意义.文章从其数字资源、技术特征、服务特点等方面进行了概要的评述,并给出作者的评价和建议.

  12. Library Resources for the Blind and Physically Handicapped: A Directory with FY 1993 Statistics on Readership, Circulation, Budget, Staff, and Collections.

    Science.gov (United States)

    Library of Congress, Washington, DC. National Library Service for the Blind and Physically Handicapped.

    The first part of this National Library Service (NLS) for the Blind and Physically Handicapped directory lists NLS network libraries and machine-lending agencies, arranged alphabetically by state. The information for each library includes its address, telephone number, librarian, hours of operation, book collection, assistive devices, special…

  13. Library Resources for the Blind and Physically Handicapped: A Directory with FY 1992 Statistics on Readership, Circulation, Budget, Staff, and Collections.

    Science.gov (United States)

    Library of Congress, Washington, DC. National Library Service for the Blind and Physically Handicapped.

    The first part of this National Library Service for the Blind and Physically Handicapped (NLS) directory lists NLS network libraries and machine-lending agencies arranged alphabetically by state. The information about each library includes address, telephone number, hours of operation, book collection, special collections, assistive devices,…

  14. 信息异化对图书馆信息资源建设的影响%The Impact of Information Alienation on the Construction of Library Information Resources

    Institute of Scientific and Technical Information of China (English)

    邵胜春

    2014-01-01

    This article discusses the influence of information alienation for the construction of library information re-sources from the following aspects including the user ’ s perception of library information resource construction ,sub-ject consciousness ,information integration service .The article expounds strategy of the control information alienation phenomenon in information resource construction from the following aspects including strengthening the training of library human consciousness ,to provide effective information resources for users based on user demand ,to carry out information resource integration service based on user demand .%从用户对图书馆信息资源建设的认知、主体人意识的培养、信息集成服务等方面探讨了信息异化对图书馆信息资源建设的影响,并从加强图书馆人主体意识的培养;以需求为导向,为用户提供有效信息资源;基于用户需求,开展信息资源集成服务等方面,阐述了图书馆在信息资源建设中控制信息异化现象的策略。

  15. A conditional piggyBac transposition system for genetic screening in mice identifies oncogenic networks in pancreatic cancer.

    Science.gov (United States)

    Rad, Roland; Rad, Lena; Wang, Wei; Strong, Alexander; Ponstingl, Hannes; Bronner, Iraad F; Mayho, Matthew; Steiger, Katja; Weber, Julia; Hieber, Maren; Veltkamp, Christian; Eser, Stefan; Geumann, Ulf; Öllinger, Rupert; Zukowska, Magdalena; Barenboim, Maxim; Maresch, Roman; Cadiñanos, Juan; Friedrich, Mathias; Varela, Ignacio; Constantino-Casas, Fernando; Sarver, Aaron; Ten Hoeve, Jelle; Prosser, Haydn; Seidler, Barbara; Bauer, Judith; Heikenwälder, Mathias; Metzakopian, Emmanouil; Krug, Anne; Ehmer, Ursula; Schneider, Günter; Knösel, Thomas; Rümmele, Petra; Aust, Daniela; Grützmann, Robert; Pilarsky, Christian; Ning, Zemin; Wessels, Lodewyk; Schmid, Roland M; Quail, Michael A; Vassiliou, George; Esposito, Irene; Liu, Pentao; Saur, Dieter; Bradley, Allan

    2015-01-01

    Here we describe a conditional piggyBac transposition system in mice and report the discovery of large sets of new cancer genes through a pancreatic insertional mutagenesis screen. We identify Foxp1 as an oncogenic transcription factor that drives pancreatic cancer invasion and spread in a mouse model and correlates with lymph node metastasis in human patients with pancreatic cancer. The propensity of piggyBac for open chromatin also enabled genome-wide screening for cancer-relevant noncoding DNA, which pinpointed a Cdkn2a cis-regulatory region. Histologically, we observed different tumor subentities and discovered associated genetic events, including Fign insertions in hepatoid pancreatic cancer. Our studies demonstrate the power of genetic screening to discover cancer drivers that are difficult to identify by other approaches to cancer genome analysis, such as downstream targets of commonly mutated human cancer genes. These piggyBac resources are universally applicable in any tissue context and provide unique experimental access to the genetic complexity of cancer.

  16. Mobile technologies for every library

    CERN Document Server

    Gleason, Ann Whitney

    2015-01-01

    If you are wondering what mobile technology adoption means for your library or how to get started, Mobile Technologies for Every Library will answer your questions! This book will detail the opportunities and pitfalls in using mobile technology in libraries. It will also address platforms, options, security, and best practices for starting new mobile services programs or improving existing services. Gleason previews many useful apps for libraries. Web links and resources are also included.

  17. Research on Information Resources Promotion and Service Work of University Library%高校图书馆信息资源推广与服务工作探究

    Institute of Scientific and Technical Information of China (English)

    王玘珺

    2016-01-01

    The age of the Internet for the development of the university library has brought challenges and opportunities. The Internet is convenient and quick the students access to information resources and network applications into the preferred way. The library as the traditional information resources reserves and to provide information on site, its nature decides the traditional library low-key introverted style, the era of today, the proliferation of Internet application libraries are beginning to more and more by the attention of the students, in the face of adversity, the university library should take the initiative to go out, make full use of the advantages of the new media applied to library information resources to promote the work to.%互联网的时代为高校图书馆的发展带来了挑战也带来了机遇。互联网的方便快捷使得大学生在获取信息资源的途径上,网络应用成了首选途径。图书馆作为传统信息资源储备和提供信息的重要场所,其性质决定着传统图书馆低调内敛的格调,如今互联网应用泛滥的时代下,图书馆开始越来越不受到大学生们的重视,面对窘境,高校图书馆应该主动走出去,充分利用新媒介的优势运用到图书馆的信息资源推广工作中去。

  18. Cloud Computing and Your Library

    Science.gov (United States)

    Mitchell, Erik T.

    2010-01-01

    One of the first big shifts in how libraries manage resources was the move from print-journal purchasing models to database-subscription and electronic-journal purchasing models. Libraries found that this transition helped them scale their resources and provide better service just by thinking a bit differently about their services. Likewise,…

  19. The SpeX Prism Library for Ultracool Dwarfs: A Resource for Stellar, Exoplanet and Galactic Science and Student-Led Research

    Science.gov (United States)

    Burgasser, Adam

    The NASA Infrared Telescope Facility's (IRTF) SpeX spectrograph has been an essential tool in the discovery and characterization of ultracool dwarf (UCD) stars, brown dwarfs and exoplanets. Over ten years of SpeX data have been collected on these sources, and a repository of low-resolution (R 100) SpeX prism spectra has been maintained by the PI at the SpeX Prism Spectral Libraries website since 2008. As the largest existing collection of NIR UCD spectra, this repository has facilitated a broad range of investigations in UCD, exoplanet, Galactic and extragalactic science, contributing to over 100 publications in the past 6 years. However, this repository remains highly incomplete, has not been uniformly calibrated, lacks sufficient contextual data for observations and sources, and most importantly provides no data visualization or analysis tools for the user. To fully realize the scientific potential of these data for community research, we propose a two-year program to (1) calibrate and expand existing repository and archival data, and make it virtual-observatory compliant; (2) serve the data through a searchable web archive with basic visualization tools; and (3) develop and distribute an open-source, Python-based analysis toolkit for users to analyze the data. These resources will be generated through an innovative, student-centered research model, with undergraduate and graduate students building and validating the analysis tools through carefully designed coding challenges and research validation activities. The resulting data archive, the SpeX Prism Library, will be a legacy resource for IRTF and SpeX, and will facilitate numerous investigations using current and future NASA capabilities. These include deep/wide surveys of UCDs to measure Galactic structure and chemical evolution, and probe UCD populations in satellite galaxies (e.g., JWST, WFIRST); characterization of directly imaged exoplanet spectra (e.g., FINESSE), and development of low

  20. Application of the Competency Model in Library Management of Human Resources%胜任力模型在图书馆人力资源管理中的应用

    Institute of Scientific and Technical Information of China (English)

    陈祥娟; 刘晓景; 熊娇

    2012-01-01

    The introduction of the competency model in library management of human resources has the extremely important significance for the development of modern library.This article,from the main ideas of the competency model analysed the significance and necessity to establish the compentcy model in library management of human resources,and provided the compentcy model in library management of human resource-based ways and means.%将胜任力模型引入图书馆人力资源管理中,对现代图书馆事业的发展具有重大意义。从胜任力模型的主要理念出发,透析出建立胜任力模型在图书馆人力资源管理中的意义与必要性,并提出基于图书馆人力资源管理构建胜任力模型的方法和途径。

  1. 基于使用数据的馆藏电子资源评价方法与系统分析%Methods and Systems of Library Electronic Resources Evaluation Analysis Based on Usage Data

    Institute of Scientific and Technical Information of China (English)

    王丹丹

    2014-01-01

    The origin and methods of library electronic resources evaluation were summarized;the statistical standards of library users' us-age data were reviewed. Then four library electronic resources evaluation systems based on library users' usage data which are ScholarlyS-tats,Journal Use Reports,360 Counter and Ustat were compared and contrasted. The related work which librarians need to do and the con-sidering factors about the selection of electronic resources evaluation system was also analyzed.%总结了图书馆馆藏电子资源评价的缘起与方法,梳理了图书馆用户使用数据的统计规范。以此为基础对比了四个基于使用数据的馆藏电子资源评价系统---ScholarlyStats,Journal Use Reports,360 Counter,UStat,分析了图书馆选择电子资源评价系统的考虑因素和需要预先做好的相关工作。

  2. 东莞图书馆数字资源的整合建设与便捷服务%The Integrated Construction and Convenient Services of Digital Resources in Dongguan Library

    Institute of Scientific and Technical Information of China (English)

    杨累

    2012-01-01

    信息社会和数字出版不断发展,数字阅读日益流行,数字资源成为公共图书馆的重要资源,如何更好地建设数字资源并开展服务成为公共图书馆面临的新课题.文章论述了东莞图书馆数字资源整合建设的内容、原则和具体措施,随之结合东莞图书馆的实践分析了一些具体的便捷服务举措,并提出了几点问题及思考.%With the continuous development of information society and digital publishing, digital reading has become increasingly popular, and digital resources have become an important resource for public libraries. As a result, how to better conduct its construction and service has become a new topic which public libraries are faced with. The article discusses the content, principle and specific initiatives of digital resources integration in Dongguan library, analyses a few specific convenient initiatives along with the practice of Dongguan library, and proposed several issues and thoughts.

  3. Books, children, dogs, artists: library programs for the entire family.

    Science.gov (United States)

    Haver, Mary Katherine

    2014-01-01

    The promotion of library resources and services is a continuous process for all libraries, especially hospital family resource center libraries. Like public libraries, a family resource center can utilize programs as a pathway for connecting with and developing awareness of library resources and services available to patient families. This column describes the programs currently offered for All Children's Hospital Johns Hopkins Medicine patient families, marketing initiatives to promote these programs, and utilization of grant funding to supplement a program.

  4. Strengthen the Building of Special Resources to Enhance College Library Service Level%加强特色资源建设 提升高职院校图书馆服务水平

    Institute of Scientific and Technical Information of China (English)

    许翠珍

    2012-01-01

    随着信息技术的快速发展,对图书馆的馆藏结构和服务模式都提出了新的要求,本文阐述了高职院校图书馆特色资源数据库的建设和提升服务水平的相互依存关系。%With the rapid development of information technology,there is new requirements for the library's collection structure and service model.This essay describes the connection betweenthe library special resources databases construction and enhancing of the library service level.

  5. 高校图书馆电子资源购买过程中的委托-代理关系探究%Principal-agent Relationship During the Electronic Resources Purchase Process of University Libraries

    Institute of Scientific and Technical Information of China (English)

    桂君; 吴冬曼

    2012-01-01

    The prineipal-agent relationship is throughout the whole process of the electronic resources purchase for university libraries. Based on the asymmetric informatiun theory the issues of information asymmetries in the purchase process of electronic resources for university libraries are analyzed. The major prineipal-agent relationships between the university libraries, the group organizers and the database providers ate studied. The operational characteristics and existing problems in the purchase process of electronic resources for university libraries are also discussed. Taking this opportunity to attention the principal-agent relationships existed in the electronic resources purchase of university libraries, the authors hope to solve some problems caused by the market information asymmetry.%委托-代理关系贯穿了高校图书馆电子资源的购买全过程,,基于信息不对称理论,分析高校图书馆电子资源购买中的信息不对称现象,并以高校图书馆、集团组织者、数据库提供者为主体,研究高校图书馆电子资源采购过程中存在的几种主要委托-代理关系、业务特点和存在的问题、通过对委托-代理关系的研究和分析,合理把握电子资源采购的规律,可以部分解决高校电子资源购买中由市场信息不平衡所带来的问题。

  6. Investigating and Analyzing on the Utilization Status of the Digital Information Resources in the Libraries of Key Universities in Western China---Taking the Library of Shihezi University as an Example%西部重点高校图书馆电子资源利用的调查及分析--以石河子大学图书馆为例

    Institute of Scientific and Technical Information of China (English)

    刘皓

    2015-01-01

    ABSTRACT:Based on introducing the general situation of the library of Shihezi University, and adopting the method of questionnaire survey, this paper investigates the awareness of the teachers and the students of Shihezi University to the digital resources of the library and the utilization ratio of the database of various digital resources, and based on the results of the investigation and analysis, points out that university library should strengthen the related propaganda of all kinds of database of digital resources, continuously increase the purchase of digital resources, and optimize the configuration of all kinds of professional electronic resources for meeting the information demands of teachers and students.%在介绍石河子大学图书馆概况的基础上,以问卷调查的方法,调查了该校师生对图书馆电子资源的认知情况和各类电子资源数据库的利用率,根据分析调查结果,指出高校图书馆应加强各类电子资源数据库的相关宣传,持续加大对电子资源的采购力度,并优化各类专业电子资源的配置,以满足师生的信息需求。

  7. Wyoming: Open Range for Library Technology.

    Science.gov (United States)

    Maul, Helen Meadors

    1996-01-01

    Describes the development of library technology and the need for telecommunications in a state with a lack of population density. Topics include the state library's role; shared library resources and library networks; government information; the Wyoming State Home Page on the World Wide Web; Ariel software; network coordinating; and central…

  8. Illinois: Library Automation and Connectivity Initiatives.

    Science.gov (United States)

    Lamont, Bridget L.; Bloomberg, Kathleen L.

    1996-01-01

    Discussion of library automation in Illinois focuses on ILLINET, the Illinois Library and Information Network. Topics include automated resource sharing; ILLINET's online catalog; regional library system automation; community networking and public library technology development; telecommunications initiatives; electronic access to state government…

  9. National Air and Space Museum Library.

    Science.gov (United States)

    Pietropaoli, Frank A.

    1986-01-01

    A branch of Smithsonian Institution Libraries, the National Air and Space Museum Library provides materials and services to support research programs of National Air and Space Museum. Brief histories of museum and its library and summary of museum programs provide background for overview of current library users, resources, and services.…

  10. Putting the Library at Students' Fingertips

    Science.gov (United States)

    Foley, Marianne

    2012-01-01

    The absence of a well-defined space for library resources within course-management systems has been well documented in library literature. Academic libraries have sought to remedy this deficiency in numerous ways. This article describes how a "library nugget," or module, was added to the Buffalo State College course-management system, ANGEL. The…

  11. Library Employment Sources on the Internet

    Science.gov (United States)

    Barr, Catherine

    2012-01-01

    This article presents a list of online resources for library job seekers. This includes general sites/portals like American Library Association (ALA): Education & Careers and Canadian Library Association: Library Careers. It also includes sites by sector, employment agencies/commercial services, listservs and networking sites.

  12. A Library Media Guide for Vermont Schools.

    Science.gov (United States)

    Vermont State Dept. of Education, Montpelier. Div. of Federal Assistance.

    This guide is designed to be used in developing and improving school library media programs and in teaching students how to use the library. Topics covered include: general philosophy; specific goals; roles and responsibilities; resources needed; creating a library media center; curriculum (including a brief scope and sequence of library skills);…

  13. On the development and the utilization of library holding in electronic resources Taking the Library of Shandong University of Science and Technology%试论电子资源的馆藏发展与利用——以山东科技大学图书馆为例

    Institute of Scientific and Technical Information of China (English)

    陈明华; 马圣芹

    2012-01-01

    通过分析电子资源馆藏发展的意义及模式,对电子资源发展的目标及有效管理进行了探讨,并提出一些提高电子资源利用率的措施。%This paper analyses the mode and the importance of library holding development in electronic resources. It also ex- plores the aim and the affective management in electronic resource development and put forwards some measures to improve the utilization ratio of electronic resources.

  14. Construction and characterization of a bacterial artificial chromosome library of thermo-sensitive genic male-sterile rice 5460S

    Institute of Scientific and Technical Information of China (English)

    邱芳; 金德敏; 伏健民; 张超良; 谢纬武; 王斌; 杨仁崔; 张洪斌

    1999-01-01

    In order to develop a detailed physical map of the thermo-sensitive genie male-sterile (TGMS) gene-encompassing region and finally clone the TGMS gene, a high-quality rice bacterial artificial chromosome (BAC) library from TGMS rice 5460S was constructed. The method of constructing BAC library was examined and optimized. The 5460S library consists of 19 584 BAC clones with an average insert size of 110 kb, which represents about 5 times rice haploid genome equivalents. Rice inserts of up to 140 kb and 250 kb were isolated and appeared stable after 100 generations of serial growth. Hybridization of BAC clones with mitochondrial and chloroplastic genes as probes demonstrated that this library has no organellar contamination. The 5460S library was screened with 3 molecular markers linked to tmsl gene as probes and at least 1 BAC clone was identified with each probe. The insert ends of positive clones were successfully isolated using thermal asymmetric interlaced PCR (TAIL-PCR) technique.

  15. Clinical comparison of the Bactec Mycosis IC/F, BacT/Alert FA, and BacT/Alert FN blood culture vials for the detection of candidemia.

    Science.gov (United States)

    Ericson, Eva-Lena; Klingspor, Lena; Ullberg, Måns; Ozenci, Volkan

    2012-06-01

    The present study analyzed the performance of Bactec Mycosis IC/F, BacT/Alert FA, and BacT/Alert FN vials in detection and time to detection (TTD) of Candida spp. in 179 simultaneous blood cultures. The Mycosis IC/F, BacT/Alert FA, and BacT/Alert FN vials could detect Candida spp. in 144 (80.45%) of 179, 149 (83.24%) of 179, and 8 (4.47%) of 179 samples, respectively. With the presence of antifungal therapy, the numbers of positive vials were higher in BacT/Alert FA compared to Mycosis IC/F, 87/99 versus 73/99, respectively (P IC/F than in BacT/Alert FA vials without antifungal therapy, 20.89 (9.33) versus 28.26 (9.77), respectively (P IC/F than in BacT/Alert FA vials, 28/30 and 19/30, respectively (P = 0.01). The present data show that the use of Bactec Mycosis IC/F together with BacT/Alert FA vials might improve the detection of Candida spp.

  16. An active piggyBac-like element in Macdunnoughia crassisigna

    Institute of Scientific and Technical Information of China (English)

    Min Wu; Zhi-Chan Sun; Chun-Lin Hu; Gu-Feng Zhang; Zhao-Jun Han

    2008-01-01

    In this paper,a highly conserved piggyBac-like sequence.designated as McrPLE was cloned from a lepidopteran insect,Macdunnoughia crassisigna.It is 2 472 bp long in full length with a single open reading frame and encodes a 595 amino acid transPOsase.It shares identical terminal and sub-terminal repeats with T. ni IFP2 and iS flanked by the typical TTAA target-site duplications.Alignment and phylogeneric analysis revealed that McrPLE had greater than 99.5%identity and appeared to be the closest one in phylogeny to IFP2 among the PLEs so far found in various species.Plasmid.based excision and transposition assay proved it Was mobile in cell culture.Otherwise.McPLE element and all other highly conserved IFP2 sequences reported previously were found to share three corrution nucleotide substitutions.This suggests that the original IFP2 may be a related variant of a predecessor element that became widespread.The existence of nearly identical piggyBac sequence in reproductively isolated species Was thought also a strong indication of horizontal transmission,which raises important considerations for the stability and practical use of piggyBac transformation vectors.

  17. The BepiColombo Archive Core System (BACS)

    Science.gov (United States)

    Macfarlane, A. J.; Osuna, P.; Pérez-López, F.; Vallejo, J. C.; Martinez, S.; Arviset, C.; Casale, M.

    2015-09-01

    BepiColombo is an interdisciplinary ESA mission to explore the planet Mercury in cooperation with JAXA. The mission consists of two separate orbiters: ESA's Mercury Planetary Orbiter (MPO) and JAXA's Mercury Magnetospheric Orbiter (MMO), which are dedicated to the detailed study of the planet and its magnetosphere. The MPO scientific payload comprises 11 instruments covering different scientific disciplines developed by several European teams. The MPO science operations will be prepared by the MPO Science Ground Segment (SGS) located at the European Space Astronomy Centre (ESAC) in Madrid. The BepiColombo Archive Core System (BACS) will be the central archive in which all mission operational data will be stored and is being developed by the Science Archives and Virtual Observatory Team (SAT) also at ESAC. The BACS will act as one of the modular subsystems within the BepiColombo Science Operations Control System (BSCS), (Vallejo 2014; Pérez-López 2014) which is under the responsibility of the SGS, with the purpose of facilitating the information exchange of data and metadata between the other subsystems of the BSCS as well as with the MPO Instrument Teams. This paper gives an overview of the concept and design of the BACS and how it integrates into the science ground segment workflow.

  18. 武警院校多媒体资源库建设的现状及对策%The status quo and countermeasures of multimedia resources library construction in armed police force academies

    Institute of Scientific and Technical Information of China (English)

    何建鹏; 王基生

    2011-01-01

    Based on the survey results of multimedia resources library construction in related academies amongtechnieal staff participating in armed police force educational technology training, the paper tries to identify the main factors affecting and constraining the construction of academic multimedia resources library. It also brings forward some countermeasures and suggestions for multimedia resources construction in order to improve the teaching support of multimedia resources library and provide better services for multimedia-assisted teaching.%通过对参加武警部队教育技术骨干培训的人员进行有关院校多媒体教育资源建设现状的调查,分析了影响和制约多媒体资源库建设的主要因素,提出了对策与建议,以提高多媒体资源库的教学保障能力,更好地为多媒体教学服务.

  19. Investigation on the Construction of Digital Resources in Libraries of Mining Colleges and Universities%矿业类高校图书馆数字资源建设调查研究

    Institute of Scientific and Technical Information of China (English)

    李红梅

    2014-01-01

    通过访问11所隶属于原煤炭部的矿业类高等院校的图书馆网站,对其数据库的数量、引进数据库、自建数字资源以及数字资源的组织方式等进行了统计分析,以期能够反映出我国矿业类高等院校图书馆数字资源建设与利用情况,为矿业类院校图书馆在数字资源建设与利用方面相互借鉴提供参考。%Through accessing to the websiets of the libraries of 11 coal mining colleges and universities affiliated with the former Ministry of Coal Industry, this paper makes a statistical analysis on database number, introduces database, self-built digital resources, and the organization form of digital resources, etc., hoping to reflect the current construction and utilization of digital resources in the libraries of coal mining colleges and universities in China so as to provide a reference in the construction and utilization of digital resources in the libraries of coal mining colleges and universities .

  20. PR: Priority for Community College Libraries.

    Science.gov (United States)

    Vaughan, Elinor F.

    1987-01-01

    Describes the Stanly Technical College Library's public relations program which attempts to encourage the use of library and media resources and to promote the college as a major community resource. Highlights library activities, including a photography contest for students and staff, and displays featuring college programs and local history. (DMM)

  1. Parent Involvement in Elementary School Libraries

    Science.gov (United States)

    Copeland, Michele Rzewski

    2012-01-01

    In the 21st century, school libraries are under pressure to innovate. Library budgets are frequently slashed as districts struggle with limited fiscal resources, while library personnel are increasingly expected to provide students with resources they need to help them pass high stakes tests. In an effort to meet student needs with limited…

  2. Validation of the tablet-administered Brief Assessment of Cognition (BAC App).

    Science.gov (United States)

    Atkins, Alexandra S; Tseng, Tina; Vaughan, Adam; Twamley, Elizabeth W; Harvey, Philip; Patterson, Thomas; Narasimhan, Meera; Keefe, Richard S E

    2016-10-19

    Computerized tests benefit from automated scoring procedures and standardized administration instructions. These methods can reduce the potential for rater error. However, especially in patients with severe mental illnesses, the equivalency of traditional and tablet-based tests cannot be assumed. The Brief Assessment of Cognition in Schizophrenia (BACS) is a pen-and-paper cognitive assessment tool that has been used in hundreds of research studies and clinical trials, and has normative data available for generating age- and gender-corrected standardized scores. A tablet-based version of the BACS called the BAC App has been developed. This study compared performance on the BACS and the BAC App in patients with schizophrenia and healthy controls. Test equivalency was assessed, and the applicability of paper-based normative data was evaluated. Results demonstrated the distributions of standardized composite scores for the tablet-based BAC App and the pen-and-paper BACS were indistinguishable, and the between-methods mean differences were not statistically significant. The discrimination between patients and controls was similarly robust. The between-methods correlations for individual measures in patients were r>0.70 for most subtests. When data from the Token Motor Test was omitted, the between-methods correlation of composite scores was r=0.88 (df=48; pBAC App generates results consistent with the traditional pen-and-paper BACS, and support the notion that the BAC App is appropriate for use in clinical trials and clinical practice.

  3. A BAC clone of MDV strain GX0101 with REV-LTR integration retained its pathogenicity

    Institute of Scientific and Technical Information of China (English)

    SUN AiJun; LAWRENCE Petherbridge; ZHAO YuGuang; LI YanPeng; NAIR Venugopal K; CUI ZhiZhong

    2009-01-01

    The complete genome of Marek's disease virus (MDV) strain GX0101,which was integrated with the LTR sequences of REV,was cloned in Escherichia coli as a bacterial artificial chromosome (BAC).BAC vector sequences were introduced into the US2 locus of the MDV genome by homologous recombination.The viral DNA containing the BAC vector was used to transform Escherichia coli strain of DH10B.Then the recombinant virus was successfully rescued by transfection of the recombinant BAC DNA into primary chicken embryo fibroblast (CEF).This BAC viral clone was named bac-GX0101.When the reconstituted virus was inoculated into 1-day-old birds,visceral tumors could be detected as early as 62 d post infection.There was no difference in growth ability and pathogenicity to birds between the BAC derived virus and its parental virus.The BAC derived virus maintained its oncogenicity and immunosuppressive effects.In conclusion,the complete genome of GX0101 strain was successfully cloned into BAC and the infectious clone was rescued.With the powerful BAC manipulation system,the infectious clone will provide a useful tool for further understanding the functional roles of the inserted REV-LTR sequence in the GX0101 strain of MDV.

  4. 基于高校图书馆电子资源的采访调查与分析——以海南大学图书馆为例%Survey and Analysis Based on the Electronic Resource of University Libraries Taking Hainan University library as an Example

    Institute of Scientific and Technical Information of China (English)

    杨莹

    2011-01-01

    随着信息化时代的到来,高校图书馆的馆藏资源建设模式已经发生了变化,形成了纸质资源与电子资源并存的局面,电子资源以其方便快捷深受读者喜爱,电子资源的采购比例越来越重,如何协调电子资源的采购比例,是图书馆界丞待解决的问题.文章通过对海南大学2006-2010年间的电子资源经费使用情况进行统计、对比和分析,对此问题进行了初步的探讨.%With the arrival of information age, college library collection resources construction mode has changed, and the paper-based resources and electroic resources coexist.With the convenience of electronic resources, the acquisition proportion of electronic resources grows.How to coordinate the acquisition of electronic resource proportion is a problem faced by the libraries.Through the funds statistics, contrast and analysis of electronic resources in hainan university 2006-2010 this article provides a preliminary discussion.

  5. [Expression of limulus Factor C in silkworm larvae by Bac-to-Bac/BmNPV baculovirus expression system].

    Science.gov (United States)

    Qi, Jing; Liu, Tao; Li, Zhen; Gong, Chengliang; Wu, Haiping; Zhang, Chun

    2014-10-01

    Limulus Factor C, a serine protease zymogen from the amoebocytes of the limulus, has high affinity for endotoxin. When Factor C is activated by endotoxin, it hydrolyses artificial tripeptide substrate and measurable products are released, so it can be used as an alternative reagent for endotoxin analysis. Factor C gene of Tachypleus tridentatus was obtained through RT-PCR and the recombinant protein was expressed by Bac-to-Bac/BmNPV baculovirus expression system in silkworm larvae. The activity of Factor C was detected with diluted serum of silkworm larvae, and the sensitivity of endotoxin detected was 0.2 EU/mL when the serum was diluted at 1:500. The silkworm larvae expressed limulus Factor C could be used to develop a new low-cost endotoxin test reagent.

  6. 图书情报领域学术深网资源利用探究%Research on the Utilization of Academic Invisible Web Resources in the Field of Li-brary and Information Science

    Institute of Scientific and Technical Information of China (English)

    顾东晓; 盛东方

    2016-01-01

    文章采用引文分析法,探究了图书情报领域研究对学术深网资源(AIW资源)的利用情况与趋势。文章首先界定学术深网资源的内涵与特征,爬取近五年出现在领域权威期刊中的学术深网资源引文作为研究样本,进而从学术深网资源引文的总体情况、资源类型、和资源来源三个层面进行分析,最后对科研资源的聚合与服务建设发展提出了建议。%This study explored the utilization of academic invisible web resources in the field of library and information science via the method of citation analysis. First, the connotation and characteristics of the academic invisible web re-sources were defined. Then this paper analyzed the overall situation, resources types, and resources of the academic in-visible web resources based on a sample of academic invisible web citation published on authoritative journals in the field of library and information science in last five years. Some suggestions were put forward for the aggregation and service of scientific research resources.

  7. 重庆高校图书馆电子文献资源建设现状及对策%The Present Situation and Countermeasure of Electronic Literature Resources Construction about Chongqing University Library

    Institute of Scientific and Technical Information of China (English)

    刘子辉; 彭渝; 陈强; 吴晓英

    2014-01-01

    In the information society, Electronic document resources are very important for library development and the school's teaching and research. The university library should pay more at ention to the construction of electronic document resources. Through the survey of the electronic document resources in Chongqing, We have mastered the distribution of electronic document resources, analyzed the existing problems in resource construction, and proposed the related countermeasure. We hope to provide reference for the electronic document resources construction.%在信息社会,电子文献资源对高校图书馆建设、学校的教学与科研都起到非常重要的作用,各高校图书馆应该更加重视电子文献资源的建设。通过对重庆高校图书馆电子文献资源建设情况进行调查,掌握了本地区电子文献资源的分布情况,分析了资源建设中存在的问题,并提出了相关对策,希望为本地区高校图书馆电子文献资源建设提供参考。

  8. 图书馆纸质资源与电子资源投入比例研究%The research of ratio between traditional and electronics resources in library

    Institute of Scientific and Technical Information of China (English)

    王以婧; 李明

    2012-01-01

    With the popularization of electronic product development,library is no longer the paper-based resources into a single information carrier the pattern,but has formed a paper resource and electronic resources coexist in the new situation.Because the library types of our country,the proportion of input between paper-based resources and other kinds of resources exist difference.This paper will discuss the relationship between the traditional and electronics resources,and also points out the existing problems and the countermeasures.%随着电子产品的普及发展,图书馆已不再以纸质资源为唯一的信息载体,而是形成了纸质资源和电子资源并存的新局面。由于国内图书馆类型的不同,纸质资源与电子资源的配比也会存在差异。通过讨论二者的关系,指出存在的问题及应对之策。

  9. Engineering Faculty Indicate High Levels of Awareness and Use of the Library but Tend to Consult Google and Google Scholar First for Research Resources

    OpenAIRE

    2016-01-01

    A Review of: Zhang, L. (2015). Use of library services by engineering faculty at Mississippi State University, a large land grant institution. Science & Technology Libraries, 34(3), 272-286. http://dx.doi.org/10.1080/0194262X.2015.1090941 Objective – To investigate the engineering faculty’s information-seeking behaviour, experiences, awareness, and use of the university library. Design – Web-based survey questionnaire. Setting – The main campus of a state university in the ...

  10. Research on Higher Vocational College Library Alliance and Information Resources Maximization%高职院校图书馆联盟与信息资源最大化研究

    Institute of Scientific and Technical Information of China (English)

    张冰

    2012-01-01

    以地校跨系统合作建馆模式为例,用实证法总结了当今高职院校图书馆与社区建立的地校跨系统联盟的可行性、迫切性,在制定校、地合作办馆开展信息资源服务的策略中,指出地校跨系统合作建馆,是进行高职图书馆资源最大化,合作双赢的又一新途径。%Co-constructed cooperation library across system as example. By the empirical method the feasibility and urgency of cross system union between current higher vocational college library and community were summarized, and in the setting strategy of information resources service between libraries of school cooperation, it was pointed out the library construction cross system was to maximize the information resource, with win-win effects.

  11. 基于SWOT分析的高校图书馆数字资源云存储模式探讨%The SWOT Analysis of the Digital Resources of University Library Based on Cloud Storage

    Institute of Scientific and Technical Information of China (English)

    彭文

    2014-01-01

    First elaborated the current situation of library digital resources storage, problems and future resource storage requirements, on this basis, using swot analysis of cloud storage brings to the University Library opportunities and threats, as well as the application of cloud storage library's own advantages and disadvantages, and finally the formation of swot analysis matrix, and puts forward four kinds of strategies of University Library Application cloud development of storage.%本文首先阐述了目前图书馆数字资源存储现状、面临的问题和未来资源存储需求,在此基础上,采用SWOT方法分析云存储给高校图书馆带来的机遇和威胁,以及图书馆自身应用云存储的优势和劣势,最后形成SWOT分析矩阵,并提出高校图书馆应用云存储发展的四种战略对策。

  12. Investigation and Analytic Research on Digital Resources Navigation of University Library Websites in Zhejiang Province%浙江省高校图书馆网站数字资源导航调查和分析研究

    Institute of Scientific and Technical Information of China (English)

    胡珺; 陆屹琳

    2015-01-01

    ABSTRACT:Along with the popularization and use of university’s digital library mode, improving the network resources service quality has become the focus of library management. Connecting with the actual situation of the construction of university library websites in Zhejiang Province, this paper reveals some main problems existing in the utilization of websites’ digital resources navigation, and probes into the coping digitization strategies in line with the sustainable development of university library.%随着高校数字图书馆模式的普遍推广与使用,提升网络资源服务质量成为图书馆管理的重点。结合浙江省高校图书馆网站建设实况,揭示了网站数字资源导航利用中存在的主要问题,探讨了符合高校图书馆可持续发展的数字化应对策略。

  13. On the Cooperative Preservation of Printed Documentary Resources in the Central-Branch Libraries System%总分馆体制下印刷型文献资源协同保存探讨

    Institute of Scientific and Technical Information of China (English)

    罗婉娜

    2013-01-01

    通过对图书馆印刷型文献馆藏现状、总分馆体制发展趋势的分析,结合国内外图书馆多年实践经验,以东莞为例,探讨图书馆在总分馆体制管理架构下,以协同保存的方式解决区域内图书馆不断增长的印刷型文献的保存、利用问题。%Through analysis of the current collection of printed documentary resources and the development situations of central-branch libraries system , and of the experience of domestic and foreign libraries for years , this paper, taking Dongguan as an example, probes into how to solve the problems of the preservation and utilization of the increasingly large number of printed docu -mentary resources in the regional library under the central-branch libraries system with a cooperative preservation mode .

  14. Building the Digital Branch A Library Technology Report

    CERN Document Server

    King, David Lee

    2009-01-01

    This issue of Library Technology Reports explores the idea of the digital branch-a library website that is a vital, functional resource for patrons and enhances the library's place within its community.

  15. Analysis on the Thinking Modes of Medical Users Utilizing Library Electronic Resources%医药学用户使用图书馆电子资源的思维模式分析

    Institute of Scientific and Technical Information of China (English)

    陈东滨; 应峻; 辛继宾; 徐一新

    2012-01-01

    The paper studies the thinking modes of medical users utilizing electronic resources in college and university libraries. Combining with the questionnaires,in - depth analysis of utilization desire,utilization decision,impact factors of utilization behaviors are compiled,in order to provide references for college and university libraries formulating electronic resources marketing strategies.%研究高校图书馆医药学用户在使用电子资源过程中的思维模式,结合问卷调查结果深入分析思维模式中使用意愿、使用决策、使用行为的影响因素,为高校图书馆制定电子资源营销策略提供依据。

  16. 提高高职院校图书馆馆藏资源利用率对策研究%Strategy Research on Improving Utilization Rate of Library Collection Resources in Higher Vocational Colleges

    Institute of Scientific and Technical Information of China (English)

    李其港

    2014-01-01

    Through the surveys on the purposes, utilization degree, reading tendency and demand the ability of utilization and influence factors of library collection resources, this paper makes a conclusion. Based on the conclusion, it makes a system research to improve the utilization rate of library collection resources and puts forward the corresponding measures and thoughts.%通过对高职院校图书馆就馆藏资源利用的目的、利用程度、阅读倾向、阅读需求、利用能力以及影响因素等方面加以调查,结论为依据,对提高馆藏资源利用率的对策进行了系统的研究,提出了针对性的工作措施和思路。

  17. Co-construction and Sharing of Collection Resources among Medical University and Local Hospital Libraries%高等医学院校与地方医院馆藏资源的共建共享

    Institute of Scientific and Technical Information of China (English)

    赵旦

    2009-01-01

    The paper discusses the necessity and the feasibility of co - construction and sharing of collection resources among medical university and local hospital libraries, proposes concrete measures of its implementing, including cultivating overall situation awareness, strengthening resources construction and highlighting the characteristics of collections and implementing unified library card.%论述高等医学院校与地方医院图书馆资源共建共享的必要性和可行性,提出具体实施措施,包括树立大局意识、加强资源建设突出特色馆藏、实施通用借书证等.

  18. Information Activities in Medical Library : Tokyo Women's Medical College Library

    Science.gov (United States)

    Nishioka, Masayuki

    The library facilities, resource materials, training of librarians and so on are described at first. The library collection is that of middle sized medical library. However, since the facilities are not enough to handle it, it is necessary for the library to be supplemented by information services. Then primary information services such as reading of materials, interlibrary loan and journal acquisition system of the recent issues for each laboratory is outlined. Secondary information services centered around on-line information retrieval service, contents sheet service and preparation of index cards are also described. What a medical library should be is considered in terms of its relation to information services.

  19. Construction of a full bacterial artificial chromosome (BAC) library of Oryza sativa genome

    Institute of Scientific and Technical Information of China (English)

    TAOQUANZHOU; HAIYINGZHAO; 等

    1994-01-01

    We have constructed a full BAC library for the superior early indica variety of Oryza sativa,Guang Lu Ai 4.The MAX Efficiency DH10B with increased stability of inserts was used as BAC host cells.The potent pBelo BACII with double selection markers was used as cloning vector.The cloning efficiency we have reached was as high as 98%,and the transformation efficiency was raised up to 106 transformants/μg of large fragment DNA.The BAC recombinant transformants were picked at random and analyzed for the size of inserts,which turned out to be of 120 kb in length on average.We have obtained more than 20,000 such BAC clones.According to conventional probability equation,they covered the entire rice genome of 420,000 kb in length.The entire length of inserts of the library obtained has the 5-to 6-fold coverage of the genome.To our knowledge,this is the first reported full BAC library for a complex genome.

  20. Performance of BAC process for treatment of micro-polluted water

    Institute of Scientific and Technical Information of China (English)

    WANG Chen; MA Fang; SHAN Dan; YANG Ji-xian; Chang Chein-chi

    2008-01-01

    Biological activated carbon (BAC) has been developed on the granular activated carbon by immobi-lization of selected and acclimated species of bacteria to treat the micro-polluted water. The BAC removal effi-ciencies for nitrobenzene, permanganate index, turbidity and ammonia were investigated. Effects of shock load-ing and SEM (Scanning Electron Microscope) observation on BAC were studied. Baekwashing and its intensity of BAC were also discussed. The results showed that BAC took short time to start up and recover to the normal condition after shock loading. The shock loading studies showed that the removal efficiency of BAC was not completely inhibited even at high concentration of nitrobenzene. Backwashing performed once every 10- 20 d,or an average of 15 d. Backwashing intensity was 12-14 L/( s·m2) with air and 3-4 L/( s·m2) with water.