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Sample records for bac library resource

  1. Library Resources for Bac End Sequencing. Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Pieter J. de Jong

    2000-10-01

    Studies directed towards the specific aims outlined for this research award are summarized. The RPCI II Human Bac Library has been expanded by the addition of 6.9-fold genomic coverage. This segment has been generated from a MBOI partial digest of the same anonymous donor DNA used for the rest of the library. A new cloning vector, pTARBAC1, has been constructed and used in the construction of RPCI-II segment 5. This new cloning vector provides a new strategy in identifying targeted genomic regions and will greatly facilitate a large-scale analysis for positional cloning. A new maleCS7BC/6J mouse BAC library has been constructed. RPCI-23 contain 576 plates (approx 210,000 clones) and represents approximately 11-fold coverage of the mouse genome.

  2. A comprehensive BAC resource

    OpenAIRE

    Zhao, Shaying

    2001-01-01

    The Human Genome Project has generated extensive map and sequence data for a large number of Bacterial Artificial Chromosome (BAC) clones. In order to maximize the efficient use of the data and to minimize the redundant work for the research community, The Institute for Genomic Research (TIGR) comprehensive BAC resource (cBACr) (http://www.tigr.org/tdb/BacResource/BAC_resource_intro.html) was built as an expansion of the TIGR human BAC ends database. This resource coll...

  3. BAC library resources for map-based cloning and physical map construction in barley (Hordeum vulgare L.

    Directory of Open Access Journals (Sweden)

    Wu Cheng-Cang

    2011-05-01

    Full Text Available Background Although second generation sequencing (2GS technologies allow re-sequencing of previously gold-standard-sequenced genomes, whole genome shotgun sequencing and de novo assembly of large and complex eukaryotic genomes is still difficult. Availability of a genome-wide physical map is therefore still a prerequisite for whole genome sequencing for genomes like barley. To start such an endeavor, large insert genomic libraries, i.e. Bacterial Artificial Chromosome (BAC libraries, which are unbiased and representing deep haploid genome coverage, need to be ready in place. Result Five new BAC libraries were constructed for barley (Hordeum vulgare L. cultivar Morex. These libraries were constructed in different cloning sites (HindIII, EcoRI, MboI and BstXI of the respective vectors. In order to enhance unbiased genome representation and to minimize the number of gaps between BAC contigs, which are often due to uneven distribution of restriction sites, a mechanically sheared library was also generated. The new BAC libraries were fully characterized in depth by scrutinizing the major quality parameters such as average insert size, degree of contamination (plate wide, neighboring, and chloroplast, empty wells and off-scale clones (clones with 250 fragments. Additionally a set of gene-based probes were hybridized to high density BAC filters and showed that genome coverage of each library is between 2.4 and 6.6 X. Conclusion BAC libraries representing >20 haploid genomes are available as a new resource to the barley research community. Systematic utilization of these libraries in high-throughput BAC fingerprinting should allow developing a genome-wide physical map for the barley genome, which will be instrumental for map-based gene isolation and genome sequencing.

  4. Toward an Integrated BAC Library Resource for Genome Sequencing and Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Simon, M. I.; Kim, U.-J.

    2002-02-26

    We developed a great deal of expertise in building large BAC libraries from a variety of DNA sources including humans, mice, corn, microorganisms, worms, and Arabidopsis. We greatly improved the technology for screening these libraries rapidly and for selecting appropriate BACs and mapping BACs to develop large overlapping contigs. We became involved in supplying BACs and BAC contigs to a variety of sequencing and mapping projects and we began to collaborate with Drs. Adams and Venter at TIGR and with Dr. Leroy Hood and his group at University of Washington to provide BACs for end sequencing and for mapping and sequencing of large fragments of chromosome 16. Together with Dr. Ian Dunham and his co-workers at the Sanger Center we completed the mapping and they completed the sequencing of the first human chromosome, chromosome 22. This was published in Nature in 1999 and our BAC contigs made a major contribution to this sequencing effort. Drs. Shizuya and Ding invented an automated highly accurate BAC mapping technique. We also developed long-term collaborations with Dr. Uli Weier at UCSF in the design of BAC probes for characterization of human tumors and specific chromosome deletions and breakpoints. Finally the contribution of our work to the human genome project has been recognized in the publication both by the international consortium and the NIH of a draft sequence of the human genome in Nature last year. Dr. Shizuya was acknowledged in the authorship of that landmark paper. Dr. Simon was also an author on the Venter/Adams Celera project sequencing the human genome that was published in Science last year.

  5. Toward an Integrated BAC Library Resource for Genome Sequencing and Analysis; FINAL

    International Nuclear Information System (INIS)

    We developed a great deal of expertise in building large BAC libraries from a variety of DNA sources including humans, mice, corn, microorganisms, worms, and Arabidopsis. We greatly improved the technology for screening these libraries rapidly and for selecting appropriate BACs and mapping BACs to develop large overlapping contigs. We became involved in supplying BACs and BAC contigs to a variety of sequencing and mapping projects and we began to collaborate with Drs. Adams and Venter at TIGR and with Dr. Leroy Hood and his group at University of Washington to provide BACs for end sequencing and for mapping and sequencing of large fragments of chromosome 16. Together with Dr. Ian Dunham and his co-workers at the Sanger Center we completed the mapping and they completed the sequencing of the first human chromosome, chromosome 22. This was published in Nature in 1999 and our BAC contigs made a major contribution to this sequencing effort. Drs. Shizuya and Ding invented an automated highly accurate BAC mapping technique. We also developed long-term collaborations with Dr. Uli Weier at UCSF in the design of BAC probes for characterization of human tumors and specific chromosome deletions and breakpoints. Finally the contribution of our work to the human genome project has been recognized in the publication both by the international consortium and the NIH of a draft sequence of the human genome in Nature last year. Dr. Shizuya was acknowledged in the authorship of that landmark paper. Dr. Simon was also an author on the Venter/Adams Celera project sequencing the human genome that was published in Science last year

  6. Development of genomic resources for Citrus clementina: Characterization of three deep-coverage BAC libraries and analysis of 46,000 BAC end sequences

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    Talon Manuel

    2008-09-01

    Full Text Available Abstract Background Citrus species constitute one of the major tree fruit crops of the subtropical regions with great economic importance. However, their peculiar reproductive characteristics, low genetic diversity and the long-term nature of tree breeding mostly impair citrus variety improvement. In woody plants, genomic science holds promise of improvements and in the Citrus genera the development of genomic tools may be crucial for further crop improvements. In this work we report the characterization of three BAC libraries from Clementine (Citrus clementina, one of the most relevant citrus fresh fruit market cultivars, and the analyses of 46.000 BAC end sequences. Clementine is a diploid plant with an estimated haploid genome size of 367 Mb and 2n = 18 chromosomes, which makes feasible the use of genomics tools to boost genetic improvement. Results Three genomic BAC libraries of Citrus clementina were constructed through EcoRI, MboI and HindIII digestions and 56,000 clones, representing an estimated genomic coverage of 19.5 haploid genome-equivalents, were picked. BAC end sequencing (BES of 28,000 clones produced 28.1 Mb of genomic sequence that allowed the identification of the repetitive fraction (12.5% of the genome and estimation of gene content (31,000 genes of this species. BES analyses identified 3,800 SSRs and 6,617 putative SNPs. Comparative genomic studies showed that citrus gene homology and microsyntheny with Populus trichocarpa was rather higher than with Arabidopsis thaliana, a species phylogenetically closer to citrus. Conclusion In this work, we report the characterization of three BAC libraries from C. clementina, and a new set of genomic resources that may be useful for isolation of genes underlying economically important traits, physical mapping and eventually crop improvement in Citrus species. In addition, BAC end sequencing has provided a first insight on the basic structure and organization of the citrus genome and has

  7. End Sequencing and Finger Printing of Human & Mouse BAC Libraries

    Energy Technology Data Exchange (ETDEWEB)

    Fraser, C

    2005-09-27

    This project provided for continued end sequencing of existing and new BAC libraries constructed to support human sequencing as well as to initiate BAC end sequencing from the mouse BAC libraries constructed to support mouse sequencing. The clones, the sequences, and the fingerprints are now an available resource for the community at large. Research and development of new metaodologies for BAC end sequencing have reduced costs and increase throughput.

  8. BAC ends library generation for Illumina sequencing

    OpenAIRE

    sprotocols

    2015-01-01

    Bacterial artificial chromosome (BAC) libraries are still a valuable tool for de novo assembly of complex genomes, such as many plants genomes. Shotgun sequencing of BACs, individually or by pools, produces first assemblies which usually need further improvement towards finished quality. We developed a new approach to obtain BAC ends libraries for Illumina sequencing (BES), overcoming the expensive and time consuming BAC ends Sanger sequencing. This new method could be useful for improving de...

  9. Development of genomic resources for the prairie vole (Microtus ochrogaster: construction of a BAC library and vole-mouse comparative cytogenetic map

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    Young Larry J

    2010-01-01

    Full Text Available Abstract Background The prairie vole (Microtus ochrogaster is a premier animal model for understanding the genetic and neurological basis of social behaviors. Unlike other biomedical models, prairie voles display a rich repertoire of social behaviors including the formation of long-term pair bonds and biparental care. However, due to a lack of genomic resources for this species, studies have been limited to a handful of candidate genes. To provide a substrate for future development of genomic resources for this unique model organism, we report the construction and characterization of a bacterial artificial chromosome (BAC library from a single male prairie vole and a prairie vole-mouse (Mus musculus comparative cytogenetic map. Results We constructed a prairie vole BAC library (CHORI-232 consisting of 194,267 recombinant clones with an average insert size of 139 kb. Hybridization-based screening of the gridded library at 19 loci established that the library has an average depth of coverage of ~10×. To obtain a small-scale sampling of the prairie vole genome, we generated 3884 BAC end-sequences totaling ~2.8 Mb. One-third of these BAC-end sequences could be mapped to unique locations in the mouse genome, thereby anchoring 1003 prairie vole BAC clones to an orthologous position in the mouse genome. Fluorescence in situ hybridization (FISH mapping of 62 prairie vole clones with BAC-end sequences mapping to orthologous positions in the mouse genome was used to develop a first-generation genome-wide prairie vole-mouse comparative cytogenetic map. While conserved synteny was observed between this pair of rodent genomes, rearrangements between the prairie vole and mouse genomes were detected, including a minimum of five inversions and 16 inter-chromosomal rearrangements. Conclusions The construction of the prairie vole BAC library and the vole-mouse comparative cytogenetic map represent the first genome-wide modern genomic resources developed for this

  10. Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries

    Czech Academy of Sciences Publication Activity Database

    Šafář, Jan; Šimková, Hana; Kubaláková, Marie; Suchánková, Pavla; Čihalíková, Jarmila; Bartoš, Jan; Fiocchetti, F.; Roselli, M.; Gill, B. S.; Doležel, Jaroslav; Lucretti, S.

    2009-01-01

    Roč. 61, 1-2 (2009), s. 151-160. ISSN 0394-9257 R&D Projects: GA ČR GD521/05/H013; GA ČR GA521/06/1723; GA ČR GA521/07/1573; GA MŠk(CZ) LC06004; GA MŠk OC08025 Institutional research plan: CEZ:AV0Z50380511 Keywords : BAC library * Flow sorting * Homoeologous chromosomes Subject RIV: GE - Plant Breeding

  11. Construction of BAC Libraries from Flow-Sorted Chromosomes.

    Science.gov (United States)

    Šafář, Jan; Šimková, Hana; Doležel, Jaroslav

    2016-01-01

    Cloned DNA libraries in bacterial artificial chromosome (BAC) are the most widely used form of large-insert DNA libraries. BAC libraries are typically represented by ordered clones derived from genomic DNA of a particular organism. In the case of large eukaryotic genomes, whole-genome libraries consist of a hundred thousand to a million clones, which make their handling and screening a daunting task. The labor and cost of working with whole-genome libraries can be greatly reduced by constructing a library derived from a smaller part of the genome. Here we describe construction of BAC libraries from mitotic chromosomes purified by flow cytometric sorting. Chromosome-specific BAC libraries facilitate positional gene cloning, physical mapping, and sequencing in complex plant genomes. PMID:27511172

  12. BAC Library of T. pallidum DNA in E. coli

    OpenAIRE

    Šmajs, David; McKevitt, Matthew; Wang, Ling; Howell, Jerrilyn K.; Norris, Steven J; Palzkill, Timothy; Weinstock, George M.

    2002-01-01

    Treponema pallidum subspecies pallidum (Nichols) chromosomal DNA was used to construct a large insert bacterial artificial chromosome (BAC) library in Escherichia coli DH10B using the pBeloBAC11 cloning vector; 678 individual insert termini of 339 BAC clones (13.9 x coverage) were sequenced and the cloned chromosomal region in each clone was determined by comparison to the genomic sequence. A single 15.6-kb region of the T. pallidum chromosome was missing in the BAC library, between bp 248727...

  13. A novel resource for genomics of Triticeae: BAC library specific for the short arm of rye (Secale cereale L.) chromosome 1R (1RS)

    Czech Academy of Sciences Publication Activity Database

    Šimková, Hana; Šafář, Jan; Suchánková, Pavla; Kovářová, Pavlína; Bartoš, Jan; Kubaláková, Marie; Janda, Jaroslav; Čihalíková, Jarmila; Mago, R.; Lelley, T.; Doležel, Jaroslav

    2008-01-01

    Roč. 9, č. 237 (2008), s. 101-109. ISSN 1471-2164 R&D Projects: GA ČR GA521/04/0607; GA ČR GP521/05/P257; GA ČR GD521/05/H013; GA MŠk(CZ) LC06004 Institutional research plan: CEZ:AV0Z50380511 Keywords : flow cytometry * flow-sorted chromosomes * BAC library Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.926, year: 2008

  14. Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, Lates calcarifer

    OpenAIRE

    Lin Grace; Zhu Ze; Li Jian; Gong Ping; Feng Felicia; Lo Loong; Wang Chun; Yue Gen

    2008-01-01

    Abstract Background Barramundi (Lates calcarifer) is an important farmed marine food fish species. Its first generation linkage map has been applied to map QTL for growth traits. To identify genes located in QTL responsible for specific traits, genomic large insert libraries are of crucial importance. We reported herein a bacterial artificial chromosome (BAC) library and the mapping of BAC clones to the linkage map. Results This BAC library consisted of 49,152 clones with an average insert si...

  15. Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, Lates calcarifer

    Directory of Open Access Journals (Sweden)

    Lin Grace

    2008-03-01

    Full Text Available Abstract Background Barramundi (Lates calcarifer is an important farmed marine food fish species. Its first generation linkage map has been applied to map QTL for growth traits. To identify genes located in QTL responsible for specific traits, genomic large insert libraries are of crucial importance. We reported herein a bacterial artificial chromosome (BAC library and the mapping of BAC clones to the linkage map. Results This BAC library consisted of 49,152 clones with an average insert size of 98 kb, representing 6.9-fold haploid genome coverage. Screening the library with 24 microsatellites and 15 ESTs/genes demonstrated that the library had good genome coverage. In addition, 62 novel microsatellites each isolated from 62 BAC clones were mapped onto the first generation linkage map. A total of 86 BAC clones were anchored on the linkage map with at least one BAC clone on each linkage group. Conclusion We have constructed the first BAC library for L. calcarifer and mapped 86 BAC clones to the first generation linkage map. This BAC library and the improved linkage map with 302 DNA markers not only supply an indispensable tool to the integration of physical and linkage maps, the fine mapping of QTL and map based cloning genes located in QTL of commercial importance, but also contribute to comparative genomic studies and eventually whole genome sequencing.

  16. Construction of a BAC library and identification of Dmrt1 gene of the rice field eel, Monopterus albus

    International Nuclear Information System (INIS)

    A bacterial artificial chromosome (BAC) library was constructed using nuclear DNA from the rice field eel (Monopterus albus). The BAC library consists of a total of 33,000 clones with an average insert size of 115 kb. Based on the rice field eel haploid genome size of 600 Mb, the BAC library is estimated to contain approximately 6.3 genome equivalents and represents 99.8% of the genome of the rice field eel. This is first BAC library constructed from this species. To estimate the possibility of isolating a specific clone, high-density colony hybridization-based library screening was performed using Dmrt1 cDNA of the rice field eel as a probe. Both library screening and PCR identification results revealed three positive BAC clones which were overlapped, and formed a contig covering the Dmrt1 gene of 195 kb. By sequence comparisons with the Dmrt1 cDNA and sequencing of first four intron-exon junctions, Dmrt1 gene of the rice field eel was predicted to contain four introns and five exons. The sizes of first and second intron are 1.5 and 2.6 kb, respectively, and the sizes of last two introns were predicted to be about 20 kb. The Dmrt1 gene structure was conserved in evolution. These results also indicate that the BAC library is a useful resource for BAC contig construction and molecular isolation of functional genes

  17. Preliminary Study of BAC Library Construction in Black Tiger Shrimp, Penaeus monodon

    OpenAIRE

    Suwit WUTHISUTHIMETHAVEE; Aoki, Takashi; Hirono, Ikuo; Tassanakajon, Anchalee

    2009-01-01

    Availability of shrimp genome information is necessary for shrimp genetic studies and large-insert DNA clones, bacterial artificial chromosome (BACs) serve as valuable tools for obtaining genomic sequences. The construction of a BAC library was achieved from this preliminary study of P. monodon. High molecular weight (HMW) genomic DNA was isolated from abdominal muscle and the resulting hemocytes were of high quality and sufficient quantity for a BAC library construction. This BAC library was...

  18. Advancing Eucalyptus genomics: identification and sequencing of lignin biosynthesis genes from deep-coverage BAC libraries

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    Kudrna David

    2011-03-01

    Full Text Available Abstract Background Eucalyptus species are among the most planted hardwoods in the world because of their rapid growth, adaptability and valuable wood properties. The development and integration of genomic resources into breeding practice will be increasingly important in the decades to come. Bacterial artificial chromosome (BAC libraries are key genomic tools that enable positional cloning of important traits, synteny evaluation, and the development of genome framework physical maps for genetic linkage and genome sequencing. Results We describe the construction and characterization of two deep-coverage BAC libraries EG_Ba and EG_Bb obtained from nuclear DNA fragments of E. grandis (clone BRASUZ1 digested with HindIII and BstYI, respectively. Genome coverages of 17 and 15 haploid genome equivalents were estimated for EG_Ba and EG_Bb, respectively. Both libraries contained large inserts, with average sizes ranging from 135 Kb (Eg_Bb to 157 Kb (Eg_Ba, very low extra-nuclear genome contamination providing a probability of finding a single copy gene ≥ 99.99%. Libraries were screened for the presence of several genes of interest via hybridizations to high-density BAC filters followed by PCR validation. Five selected BAC clones were sequenced and assembled using the Roche GS FLX technology providing the whole sequence of the E. grandis chloroplast genome, and complete genomic sequences of important lignin biosynthesis genes. Conclusions The two E. grandis BAC libraries described in this study represent an important milestone for the advancement of Eucalyptus genomics and forest tree research. These BAC resources have a highly redundant genome coverage (> 15×, contain large average inserts and have a very low percentage of clones with organellar DNA or empty vectors. These publicly available BAC libraries are thus suitable for a broad range of applications in genetic and genomic research in Eucalyptus and possibly in related species of Myrtaceae

  19. A highly redundant BAC library of Atlantic salmon (Salmo salar: an important tool for salmon projects

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    Koop Ben F

    2005-04-01

    library. We have demonstrated the library's ability to identify specific genes and genetic markers using hybridization, PCR and fingerprinting experiments. In addition, multiple fingerprinting contigs indicated a pseudo-tetraploidity of the Atlantic salmon genome. The highly redundant CHORI-214 BAC library is expected to be an important resource for mapping and sequencing of the Atlantic salmon genome.

  20. Enhancing genome investigations in the mosquito Culex quinquefasciatus via BAC library construction and characterization

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    Saski Christopher A

    2011-09-01

    Full Text Available Abstract Background Culex quinquefasciatus (Say is a major species in the Culex pipiens complex and an important vector for several human pathogens including West Nile virus and parasitic filarial nematodes causing lymphatic filariasis. It is common throughout tropical and subtropical regions and is among the most geographically widespread mosquito species. Although the complete genome sequence is now available, additional genomic tools are needed to improve the sequence assembly. Findings We constructed a bacterial artificial chromosome (BAC library using the pIndigoBAC536 vector and HindIII partially digested DNA isolated from Cx. quinquefasciatus pupae, Johannesburg strain (NDJ. Insert size was estimated by NotI digestion and pulsed-field gel electrophoresis of 82 randomly selected clones. To estimate genome coverage, each 384-well plate was pooled for screening with 29 simple sequence repeat (SSR and five gene markers. The NDJ library consists of 55,296 clones arrayed in 144 384-well microplates. Fragment insert size ranged from 50 to 190 kb in length (mean = 106 kb. Based on a mean insert size of 106 kb and a genome size of 579 Mbp, the BAC library provides ~10.1-fold coverage of the Cx. quinquefasciatus genome. PCR screening of BAC DNA plate pools for SSR loci from the genetic linkage map and for four genes associated with reproductive diapause in Culex pipiens resulted in a mean of 9.0 positive plate pools per locus. Conclusion The NDJ library represents an excellent resource for genome assembly enhancement and characterization in Culex pipiens complex mosquitoes.

  1. Construction and characterization of a deep-coverage carrot (Daucus carota L.) BAC library

    Science.gov (United States)

    The first carrot (Daucus carota L.) BAC library was constructed using imbred line B8503, which is nematode-resistant and accumulates carotenes in its roots. The BAC library consists of 92,160 clones comprising 22.4 haploid genome equivalents based on a genome size of 473 Mb/1C. Upon the analysis of ...

  2. Construction and characterization of a Lipotes vexillifer genomic DNA BAC library.

    Science.gov (United States)

    Du, Bo; Zhang, Xian-Feng; Fang, Sheng-Guo; Wang, Ding

    2007-04-01

    We constructed a genomic DNA library for Lipotes vexillifer (L. vexillifer), the Baiji or Yangtze River dolphin, one of the most endangered mammals in the world. The library consists of 149,000 BAC clones, with an average insert size of 83 kb, representing approximately 3.4 haploid genome equivalents. PCR amplification of four known L. vexillifer genes yielded two to four positive clones each. To demonstrate the utility of this library, we isolated and sequenced the L. vexillifer alpha lactalbumin gene, which is a gene specific to mammals and one which has been widely used as molecular tool in phylogenetic analysis. We also end-sequenced 20 randomly selected clones, resulting in the identification of at least five new L. vexillifer genes, five SSR loci, and one SINE locus. These results suggest that this library is a valuable resource for candidate gene cloning, physical mapping, and genome sequencing of this important and threatened species. PMID:17867838

  3. Construction and characterization of two BAC libraries representing a deep-coverage of the genome of chicory (Cichorium intybus L., Asteraceae)

    OpenAIRE

    Gonthier Lucy; Bellec Arnaud; Blassiau Christelle; Prat Elisa; Helmstetter Nicolas; Rambaud Caroline; Huss Brigitte; Hendriks Theo; Bergès Hélène; Quillet Marie-Christine

    2010-01-01

    Abstract Background The Asteraceae represents an important plant family with respect to the numbers of species present in the wild and used by man. Nonetheless, genomic resources for Asteraceae species are relatively underdeveloped, hampering within species genetic studies as well as comparative genomics studies at the family level. So far, six BAC libraries have been described for the main crops of the family, i.e. lettuce and sunflower. Here we present the characterization of BAC libraries ...

  4. Construction and sequence sampling of deep-coverage, large-insert BAC libraries for three model lepidopteran species

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    Zhao Shaying

    2009-06-01

    Full Text Available Abstract Background Manduca sexta, Heliothis virescens, and Heliconius erato represent three widely-used insect model species for genomic and fundamental studies in Lepidoptera. Large-insert BAC libraries of these insects are critical resources for many molecular studies, including physical mapping and genome sequencing, but not available to date. Results We report the construction and characterization of six large-insert BAC libraries for the three species and sampling sequence analysis of the genomes. The six BAC libraries were constructed with two restriction enzymes, two libraries for each species, and each has an average clone insert size ranging from 152–175 kb. We estimated that the genome coverage of each library ranged from 6–9 ×, with the two combined libraries of each species being equivalent to 13.0–16.3 × haploid genomes. The genome coverage, quality and utility of the libraries were further confirmed by library screening using 6~8 putative single-copy probes. To provide a first glimpse into these genomes, we sequenced and analyzed the BAC ends of ~200 clones randomly selected from the libraries of each species. The data revealed that the genomes are AT-rich, contain relatively small fractions of repeat elements with a majority belonging to the category of low complexity repeats, and are more abundant in retro-elements than DNA transposons. Among the species, the H. erato genome is somewhat more abundant in repeat elements and simple repeats than those of M. sexta and H. virescens. The BLAST analysis of the BAC end sequences suggested that the evolution of the three genomes is widely varied, with the genome of H. virescens being the most conserved as a typical lepidopteran, whereas both genomes of H. erato and M. sexta appear to have evolved significantly, resulting in a higher level of species- or evolutionary lineage-specific sequences. Conclusion The high-quality and large-insert BAC libraries of the insects, together

  5. Wheat chromosomes sorting: dividing a komplex genome into sub-genomic specific BAC libraries

    Czech Academy of Sciences Publication Activity Database

    Lucretti, S.; Doležel, Jaroslav

    2005. P. 2.11. [Italian Society of Agricultural Genetics Annual Congress . 12.09.2005-15.09.2005, Potenza] Keywords : Wheat * flow-sorted chromosomes * BAC library Subject RIV: EB - Genetics ; Molecular Biology

  6. The first insight into the salvia (lamiaceae) genome via bac library construction and high-throughput sequencing of target bac clones

    International Nuclear Information System (INIS)

    Salvia is a representative genus of Lamiaceae, a eudicot family with significant species diversity and population adaptibility. One of the key goals of Salvia genomics research is to identify genes of adaptive significance. This information may help to improve the conservation of adaptive genetic variation and the management of medicinal plants to increase their health and productivity. Large-insert genomic libraries are a fundamental tool for achieving this purpose. We report herein the construction, characterization and screening of a gridded BAC library for Salvia officinalis (sage). The S. officinalis BAC library consists of 17,764 clones and the average insert size is 107 Kb, corresponding to 3 haploid genome equivalents. Seventeen positive clones (average insert size 115 Kb) containing five terpene synthase (TPS) genes were screened out by PCR and 12 of them were subject to Illumina HiSeq 2000 sequencing, which yielded 28,097,480 90-bp raw reads (2.53 Gb). Scaffolds containing sabinene synthase (Sab), a Sab homolog, TPS3 (kaurene synthase-like 2), copalyl diphosphate synthase 2 and one cytochrome P450 gene were retrieved via de novo assembly and annotation, which also have flanking noncoding sequences, including predicted promoters and repeat sequences. Among 2,638 repeat sequences, there are 330 amplifiable microsatellites. This BAC library provides a new resource for Lamiaceae genomic studies, including microsatellite marker development, physical mapping, comparative genomics and genome sequencing. Characterization of positive clones provided insights into the structure of the Salvia genome. These sequences will be used in the assembly of a future genome sequence for S. officinalis. (author)

  7. BACTERIAL ARTIFICIAL CHROMOSOME(BAC)LIBRARIES CONSTRUCTED FROM THE GENETIC STANDARD OF UPLAND COTTON

    Science.gov (United States)

    Two BAC libraries and one plant transformation-competent BIBAC library were developed from the Gossypium hirsutum acc. TM-1 for the development of an integrative cotton physical and genetic map and other genomic applications. TM-1 is the most desirable choice for the physical map of Upland cotton be...

  8. The bacterial artificial chromosome (BAC) library of the narrow-leafed lupin (Lupinus angustifolius L.)

    Czech Academy of Sciences Publication Activity Database

    Kasprzak, A.; Šafář, Jan; Janda, Jaroslav; Doležel, Jaroslav; Wolko, B.; Naganowska, B.

    2006-01-01

    Roč. 11, - (2006), s. 396-407. ISSN 1425-8153 R&D Projects: GA MŠk(CZ) LC06004 Institutional research plan: CEZ:AV0Z50380511 Keywords : BAC * genomic DNA library * Lupinus angustifolius L. Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.238, year: 2006

  9. The infectious BAC genomic DNA expression library: a high capacity vector system for functional genomics.

    Science.gov (United States)

    Lufino, Michele M P; Edser, Pauline A H; Quail, Michael A; Rice, Stephen; Adams, David J; Wade-Martins, Richard

    2016-01-01

    Gene dosage plays a critical role in a range of cellular phenotypes, yet most cellular expression systems use heterologous cDNA-based vectors which express proteins well above physiological levels. In contrast, genomic DNA expression vectors generate physiologically-relevant levels of gene expression by carrying the whole genomic DNA locus of a gene including its regulatory elements. Here we describe the first genomic DNA expression library generated using the high-capacity herpes simplex virus-1 amplicon technology to deliver bacterial artificial chromosomes (BACs) into cells by viral transduction. The infectious BAC (iBAC) library contains 184,320 clones with an average insert size of 134.5 kb. We show in a Chinese hamster ovary (CHO) disease model cell line and mouse embryonic stem (ES) cells that this library can be used for genetic rescue studies in a range of contexts including the physiological restoration of Ldlr deficiency, and viral receptor expression. The iBAC library represents an important new genetic analysis tool openly available to the research community. PMID:27353647

  10. BAC Library Construction and Physical Mapping of Bacillus anthracis A16R

    Institute of Scientific and Technical Information of China (English)

    Zhang Da; Zhu Houchu; Huang Liuyu

    2013-01-01

    Bacillus anthracis is an endospore-forming bacterium that causes severe inhalational anthrax, and bacillus anthracis A16R is an attenuated strain derived from Bacillus anthracis A16. The development of bacterial artificial chromosome (BAC) system has allowed the construction of large insert-size DNA libraries, and the bacterial artificial chromosomes (BACs) have become the preferred large insert cloning system for genomic analysis because such libraries are characteristically stable, high in ifdelity and easy to handle. To facilitate genome studies of this bacterium, a bacterial artiifcial chromosome library (BAC) has been established from genome DNA of Bacillus anthracis A16R. This library consisted of 9 600 clones randomly selected from more than 15 000 recombinant clones carrying inserts in the plindigoBAC-5 vectors. The mean insert size was 56 kbp, representing an approximate 12-fold genome coverage, while end sequences were obtained from 700 randomly selected clones. Sequences were compared with Bacillus anthracis Ames and Bacillus cereus ATCC 14579 Genome Project databases using the NCBI BLASTN search project. And most BLASTN results showed high identities and that the sequences’ sites could be used as STSs. To construct this physical map, Excel was used for the array of STSs and some gaps of the map were iflled up by PCR walking. Artemis-V4 was used in the construction of a genome-wide physical map with 93%genome coverage. The A16R BAC library proved to be a vital tool for the generation of a map that would not only allow the subsequent sequencing of defined areas of genome, but also provide immediate access to clones that were stable and convenient for functional genomic researches.

  11. BAC Library Construction and Physical Mapping of Bacillus anthracis A16R

    Directory of Open Access Journals (Sweden)

    Da Zhang

    2013-12-01

    Full Text Available Bacillus anthracis is an endospore-forming bacterium that causes severe inhalational anthrax, and bacillus anthracis A16R is an attenuated strain derived from Bacillus anthracis A16. The development of bacterial artificial chromosome (BAC system has allowed the construction of large insert-size DNA libraries, and the bacterial artificial chromosomes (BACs have become the preferred large insert cloning system for genomic analysis because such libraries are characteristically stable, high in fidelity and easy to handle. To facilitate genome studies of this bacterium, a bacterial artificial chromosome library (BAC has been established from genome DNA of Bacillus anthracis A16R. This library consisted of 9 600 clones randomly selected from more than 15 000 recombinant clones carrying inserts in the plindigoBAC-5 vectors. The mean insert size was 56 kbp, representing an approximate 12-fold genome coverage, while end sequences were obtained from 700 randomly selected clones. Sequences were compared with Bacillus anthracis Ames and Bacillus cereus ATCC 14579 Genome Project databases using the NCBI BLASTN search project. And most BLASTN results showed high identities and that the sequences’ sites could be used as STSs. To construct this physical map, Excel was used for the array of STSs and some gaps of the map were filled up by PCR walking. Artemis-V4 was used in the construction of a genome-wide physical map with 93% genome coverage. The A16R BAC library proved to be a vital tool for the generation of a map that would not only allow the subsequent sequencing of defined areas of genome, but also provide immediate access to clones that were stable and convenient for functional genomic researches.

  12. Library resources on the Internet

    Science.gov (United States)

    Buchanan, Nancy L.

    1995-07-01

    Library resources are prevalent on the Internet. Library catalogs, electronic books, electronic periodicals, periodical indexes, reference sources, and U.S. Government documents are available by telnet, Gopher, World Wide Web, and FTP. Comparatively few copyrighted library resources are available freely on the Internet. Internet implementations of library resources can add useful features, such as full-text searching. There are discussion lists, Gophers, and World Wide Web pages to help users keep up with new resources and changes to existing ones. The future will bring more library resources, more types of library resources, and more integrated implementations of such resources to the Internet.

  13. Versatile P(acman) BAC Libraries for Transgenesis Studies in Drosophila melanogaster

    Energy Technology Data Exchange (ETDEWEB)

    Venken, Koen J.T.; Carlson, Joseph W.; Schulze, Karen L.; Pan, Hongling; He, Yuchun; Spokony, Rebecca; Wan, Kenneth H.; Koriabine, Maxim; de Jong, Pieter J.; White, Kevin P.; Bellen, Hugo J.; Hoskins, Roger A.

    2009-04-21

    We constructed Drosophila melanogaster BAC libraries with 21-kb and 83-kb inserts in the P(acman) system. Clones representing 12-fold coverage and encompassing more than 95percent of annotated genes were mapped onto the reference genome. These clones can be integrated into predetermined attP sites in the genome using Phi C31 integrase to rescue mutations. They can be modified through recombineering, for example to incorporate protein tags and assess expression patterns.

  14. Technology at Washington University School of Medicine Library: BACS, PHILSOM, and OCTANET.

    OpenAIRE

    Crawford, S; Johnson, M.F.; Kelly, E A

    1983-01-01

    A brief overview of the Bibliographic Access and Control System developed by the Washington University School of Medicine Library is presented. Because the system has been described in two previous reports, this paper focuses on its relationship to other automated programs (i.e., PHILSOM and OCTANET), education of users, evaluation of the system, and outreach to the medical center. In operation for more than two years, BACS represents the computerization of much of the managerial and operatio...

  15. Study on the Mitochondrial Genome of Sea Island Cotton (Gossypium barbadense) by BAC Library Screening

    Institute of Scientific and Technical Information of China (English)

    SU Ai-guo; LI Shuang-shuang; LIU Guo-zheng; LEI Bin-bin; KANG Ding-ming; LI Zhao-hu; MA Zhi-ying; HUA Jin-ping

    2014-01-01

    The plant mitochondrial genome displays complex features, particularly in terms of cytoplasmic male sterility (CMS). Therefore, research on the cotton mitochondrial genome may provide important information for analyzing genome evolution and exploring the molecular mechanism of CMS. In this paper, we present a preliminary study on the mitochondrial genome of sea island cotton (Gossypium barbadense) based on positive clones from the bacterial artiifcial chromosome (BAC) library. Thirty-ifve primers designed with the conserved sequences of functional genes and exons of mitochondria were used to screen positive clones in the genome library of the sea island cotton variety called Pima 90-53. Ten BAC clones were obtained and veriifed for further study. A contig was obtained based on six overlapping clones and subsequently laid out primarily on the mitochondrial genome. One BAC clone, clone 6 harbored with the inserter of approximate 115 kb mtDNA sequence, in which more than 10 primers fragments could be ampliifed, was sequenced and assembled using the Solexa strategy. Fifteen mitochondrial functional genes were revealed in clone 6 by gene annotation. The characteristics of the syntenic gene/exon of the sequences and RNA editing were preliminarily predicted.

  16. Construction of an Americn mink Bacterial Artificial Chromosome (BAC) library and sequencing candidate genes important for the fur industry

    DEFF Research Database (Denmark)

    Anistoroaei, Razvan Marian; Hallers, Boudewijn ten; Nefedov, Michael;

    2011-01-01

    consisting of 18,432 clones spotted in duplicate, have been produced for hybridization screening and are publicly available. Overgo probes derived from expressed sequence tags (ESTs), representing 21 candidate genes for traits important for the mink industry, were used to screen the BAC library......BACKGROUND: Bacterial artificial chromosome (BAC) libraries continue to be invaluable tools for the genomic analysis of complex organisms. Complemented by the newly and fast growing deep sequencing technologies, they provide an excellent source of information in genomics projects. RESULTS: Here, we...... report the construction and characterization of the CHORI-231 BAC library constructed from a Danish-farmed, male American mink (Neovison vison). The library contains approximately 165,888 clones with an average insert size of 170 kb, representing approximately 10-fold coverage. High-density filters, each...

  17. Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus

    Directory of Open Access Journals (Sweden)

    Wilson Melanie

    2003-11-01

    Full Text Available Abstract A bacterial artificial chromosome (BAC library was constructed by cloning HindIII-digested high molecular weight DNA from a gynogenetic channel catfish, Ictalurus punctatus, into the vector pBeloBAC11. Approximately 53 500 clones were arrayed in 384-well plates and stored at -80°C (CCBL1, while clones from a smaller insert size fraction were stored at -80°C without arraying (CCBL2. Pulsed-field gel electrophoresis of 100 clones after NotI digestion revealed an average insert size of 165 kb for CCBL1 and 113 kb for CCBL2. Further characterization of CCBL1 demonstrated that 10% of the clones did not contain an insert. CCBL1 provides a 7.2-fold coverage of the channel catfish haploid genome. PCR-based screening demonstrated that 68 out of 74 unique loci were present in the library. This represents a 92% chance to find a unique sequence. These libraries will be useful for physical mapping of the channel catfish genome, and identification of genes controlling major traits in this economically important species.

  18. Construction of an American mink Bacterial Artificial Chromosome (BAC library and sequencing candidate genes important for the fur industry

    Directory of Open Access Journals (Sweden)

    Christensen Knud

    2011-07-01

    Full Text Available Abstract Background Bacterial artificial chromosome (BAC libraries continue to be invaluable tools for the genomic analysis of complex organisms. Complemented by the newly and fast growing deep sequencing technologies, they provide an excellent source of information in genomics projects. Results Here, we report the construction and characterization of the CHORI-231 BAC library constructed from a Danish-farmed, male American mink (Neovison vison. The library contains approximately 165,888 clones with an average insert size of 170 kb, representing approximately 10-fold coverage. High-density filters, each consisting of 18,432 clones spotted in duplicate, have been produced for hybridization screening and are publicly available. Overgo probes derived from expressed sequence tags (ESTs, representing 21 candidate genes for traits important for the mink industry, were used to screen the BAC library. These included candidate genes for coat coloring, hair growth and length, coarseness, and some receptors potentially involved in viral diseases in mink. The extensive screening yielded positive results for 19 of these genes. Thirty-five clones corresponding to 19 genes were sequenced using 454 Roche, and large contigs (184 kb in average were assembled. Knowing the complete sequences of these candidate genes will enable confirmation of the association with a phenotype and the finding of causative mutations for the targeted phenotypes. Additionally, 1577 BAC clones were end sequenced; 2505 BAC end sequences (80% of BACs were obtained. An excess of 2 Mb has been analyzed, thus giving a snapshot of the mink genome. Conclusions The availability of the CHORI-321 American mink BAC library will aid in identification of genes and genomic regions of interest. We have demonstrated how the library can be used to identify specific genes of interest, develop genetic markers, and for BAC end sequencing and deep sequencing of selected clones. To our knowledge, this is the

  19. Construction of a nurse shark (Ginglymostoma cirratum bacterial artificial chromosome (BAC library and a preliminary genome survey

    Directory of Open Access Journals (Sweden)

    Inoko Hidetoshi

    2006-05-01

    Full Text Available Abstract Background Sharks are members of the taxonomic class Chondrichthyes, the oldest living jawed vertebrates. Genomic studies of this group, in comparison to representative species in other vertebrate taxa, will allow us to theorize about the fundamental genetic, developmental, and functional characteristics in the common ancestor of all jawed vertebrates. Aims In order to obtain mapping and sequencing data for comparative genomics, we constructed a bacterial artificial chromosome (BAC library for the nurse shark, Ginglymostoma cirratum. Results The BAC library consists of 313,344 clones with an average insert size of 144 kb, covering ~4.5 × 1010 bp and thus providing an 11-fold coverage of the haploid genome. BAC end sequence analyses revealed, in addition to LINEs and SINEs commonly found in other animal and plant genomes, two new groups of nurse shark-specific repetitive elements, NSRE1 and NSRE2 that seem to be major components of the nurse shark genome. Screening the library with single-copy or multi-copy gene probes showed 6–28 primary positive clones per probe of which 50–90% were true positives, demonstrating that the BAC library is representative of the different regions of the nurse shark genome. Furthermore, some BAC clones contained multiple genes, making physical mapping feasible. Conclusion We have constructed a deep-coverage, high-quality, large insert, and publicly available BAC library for a cartilaginous fish. It will be very useful to the scientific community interested in shark genomic structure, comparative genomics, and functional studies. We found two new groups of repetitive elements specific to the nurse shark genome, which may contribute to the architecture and evolution of the nurse shark genome.

  20. Developemnt of BAC resources for targeted analysis of the short arm of chromosome 1R (1RS)

    Czech Academy of Sciences Publication Activity Database

    Kovářová, Pavlína; Šafář, Jan; Šimková, Hana; Suchánková, Pavla; Bartoš, Jan; Janda, Jaroslav; Doleželová, Marie; Čihalíková, Jarmila; Lelley, T.; Doležel, Jaroslav

    2006. p. 81. [2nd Congress of the International Cytogenetics and Genome Society. 25.06.2006-29.06.2006, University of Kent, Canterbury] Keywords : BAC library * rye * chromosome 1R (1RS) Subject RIV: EB - Genetics ; Molecular Biology

  1. Cytogenetical anchoring of sheep linkage map and syntenic groups using a sheep BAC library

    Directory of Open Access Journals (Sweden)

    Cribiu Edmond-Paul

    2000-07-01

    Full Text Available Abstract In order to simultaneously integrate linkage and syntenic groups to the ovine chromosomal map, a sheep bacterial artificial chromosome (BAC library was screened with previously assigned microsatellites using a sheep-hamster hybrid panel and genetic linkage. Thirty-three BACs were obtained, fluorescently labelled and hybridised on sheep-goat hybrid metaphases (2n = 57. This study allowed us, (i, to anchor all linkage groups on sheep chromosomes, (ii, to give information on the probable position of the centromere on the linkage map for the centromeric chromosomes, (iii, to contradict the previous orientation of the ovine × linkage group by the mapping of BMS1008 on OARXq38. Concerning our somatic cell hybrid panel, this study resulted in the assignment of all the previously unassigned groups to ovine chromosomes and a complete characterisation of the hybrid panel. In addition, since hybridisations were performed on a sheep-goat hybrid, new marker/anchoring points were added to the caprine cytogenetic map.

  2. Maintaining Precious Library Resources.

    Science.gov (United States)

    Wiens, Janet

    2002-01-01

    Using the examples of libraries at Southeast Missouri State University and the University of North Texas, discusses the big-picture approach and extensive communication among all users necessary for library maintenance efforts. Addresses establishing a master plan, prioritizing projects, having the right staff, and communicating. (EV)

  3. Construction and characterization of two BAC libraries representing a deep-coverage of the genome of chicory (Cichorium intybus L., Asteraceae

    Directory of Open Access Journals (Sweden)

    Gonthier Lucy

    2010-08-01

    Full Text Available Abstract Background The Asteraceae represents an important plant family with respect to the numbers of species present in the wild and used by man. Nonetheless, genomic resources for Asteraceae species are relatively underdeveloped, hampering within species genetic studies as well as comparative genomics studies at the family level. So far, six BAC libraries have been described for the main crops of the family, i.e. lettuce and sunflower. Here we present the characterization of BAC libraries of chicory (Cichorium intybus L. constructed from two genotypes differing in traits related to sexual and vegetative reproduction. Resolving the molecular mechanisms underlying traits controlling the reproductive system of chicory is a key determinant for hybrid development, and more generally will provide new insights into these traits, which are poorly investigated so far at the molecular level in Asteraceae. Findings Two bacterial artificial chromosome (BAC libraries, CinS2S2 and CinS1S4, were constructed from HindIII-digested high molecular weight DNA of the contrasting genotypes C15 and C30.01, respectively. C15 was hermaphrodite, non-embryogenic, and S2S2 for the S-locus implicated in self-incompatibility, whereas C30.01 was male sterile, embryogenic, and S1S4. The CinS2S2 and CinS1S4 libraries contain 89,088 and 81,408 clones. Mean insert sizes of the CinS2S2 and CinS1S4 clones are 90 and 120 kb, respectively, and provide together a coverage of 12.3 haploid genome equivalents. Contamination with mitochondrial and chloroplast DNA sequences was evaluated with four mitochondrial and four chloroplast specific probes, and was estimated to be 0.024% and 1.00% for the CinS2S2 library, and 0.028% and 2.35% for the CinS1S4 library. Using two single copy genes putatively implicated in somatic embryogenesis, screening of both libraries resulted in detection of 12 and 13 positive clones for each gene, in accordance with expected numbers. Conclusions This

  4. Development of Chromosome-Specific BAC Resources for Genomics of Bread Wheat

    Czech Academy of Sciences Publication Activity Database

    Šafář, Jan; Šimková, Hana; Kubaláková, Marie; Čihalíková, Jarmila; Suchánková, Pavla; Bartoš, Jan; Doležel, Jaroslav

    2010-01-01

    Roč. 129, 1-3 (2010), s. 211-223. ISSN 1424-8581 R&D Projects: GA ČR GA521/07/1573; GA MŠk(CZ) LC06004 Grant ostatní: European Community’s Seventh Framework Programme(XE) FP7/2007–2013 Institutional research plan: CEZ:AV0Z50380511 Keywords : BAC library * Chromosome * DNA markers Subject RIV: GE - Plant Breeding Impact factor: 1.783, year: 2010

  5. Gram negative shuttle BAC vector for heterologous expression of metagenomic libraries

    OpenAIRE

    Kakirde, Kavita S.; Wild, Jadwiga; Godiska, Ronald; Mead, David A.; Wiggins, Andrew G.; Goodman, Robert M.; Szybalski, Waclaw; Liles, Mark R.

    2010-01-01

    Bacterial artificial chromosome (BAC) vectors enable stable cloning of large DNA fragments from single genomes or microbial assemblages. A novel shuttle BAC vector was constructed that permits replication of BAC clones in diverse Gram-negative species. The “Gram-negative shuttle BAC” vector (pGNS-BAC) uses the F replicon for stable single-copy replication in E. coli and the broad-host-range RK2 mini-replicon for high-copy replication in diverse Gram-negative bacteria. As with other BAC vector...

  6. Libraries Serving Handicapped Users Share Resources.

    Science.gov (United States)

    Ames, Jan

    1981-01-01

    Because library resources for the blind and physically handicapped are so scarce, cooperative library activities are needed to insure the best utilization of the limited resources which are available. This article describes several such cooperative efforts. (LLS)

  7. Construction of a BAC library and a physical map of a major QTL for CBB resistance of common bean (Phaseolus vulgaris L.).

    Science.gov (United States)

    Liu, S Y; Yu, K; Huffner, M; Park, S J; Banik, M; Pauls, K P; Crosby, W

    2010-07-01

    A major quantitative trait loci (QTL) conditioning common bacterial blight (CBB) resistance in common bean (Phaseolus vulgaris L.) lines HR45 and HR67 was derived from XAN159, a resistant line obtained from an interspecific cross between common bean lines and the tepary bean (P. acutifolius L.) line PI319443. This source of CBB resistance is widely used in bean breeding. Several other CBB resistance QTL have been identified but none of them have been physically mapped. Four molecular markers tightly linked to this QTL have been identified suitable for marker assisted selection and physical mapping of the resistance gene. A bacterial artificial chromosome (BAC) library was constructed from high molecular weight DNA of HR45 and is composed of 33,024 clones. The size of individual BAC clone inserts ranges from 30 kb to 280 kb with an average size of 107 kb. The library is estimated to represent approximately sixfold genome coverage. The BAC library was screened as BAC pools using four PCR-based molecular markers. Two to seven BAC clones were identified by each marker. Two clones were found to have both markers PV-tttc001 and STS183. One preliminary contig was assembled based on DNA finger printing of those positive BAC clones. The minimum tiling path of the contig contains 6 BAC clones spanning an estimated size of 750 kb covering the QTL region. PMID:20419470

  8. Creation of a BAC resource to study the structure and evolution of the banana (Musa balbisiana) genome

    Czech Academy of Sciences Publication Activity Database

    Šafář, Jan; Noa-Carrazana, J. C.; Vrána, Jan; Bartoš, Jan; Alkhimova, Olena; Lheureux, F.; Šimková, Hana; Caruana, M. L.; Doležel, Jaroslav; Piffanelli, P.

    2004-01-01

    Roč. 47, - (2004), 1182ů1191. E-ISSN 1480-3321 R&D Projects: GA AV ČR IAA6038201 Grant ostatní: research contract IAEA(FR) 12230/RBF Institutional research plan: CEZ:AV0Z5038910 Keywords : bacterial artificial chromosome library * banana * BAC-FISH Subject RIV: EB - Genetics ; Molecular Biology

  9. BAC library development, and clone characterization for dormancy-responsive DREB4A, DAM, and FT from leafy spurge (Euphorbia esula L.) identifies differential splicing and conserved promoter motifs

    Science.gov (United States)

    We developed two leafy spurge BAC libraries that together represent approximately 5X coverage of the leafy spurge genome. The BAC libraries have an average insert size of approximately 143 kb, and copies of the library and filters for hybridization-based screening are publicly available through the ...

  10. A Bac Library and Paired-PCR Approach to Mapping and Completing the Genome Sequence of Sulfolobus Solfataricus P2

    DEFF Research Database (Denmark)

    She, Qunxin; Confalonieri, F.; Zivanovic, Y.;

    2000-01-01

    The original strategy used in the Sulfolobus solfatnricus genome project was to sequence non overlapping, or minimally overlapping, cosmid or lambda inserts without constructing a physical map. However, after only about two thirds of the genome sequence was completed, this approach became counter...... selected for walking over small gaps and preparing template libraries for larger ones. It is concluded that an optimal strategy for sequencing microorganism genomes involves construction of a high-resolution physical map by BAC end analyses, PCR screening and paired-PCR chromosome walking after about half...... the genome sequence has been accumulated....

  11. New approaches for genome characterization: genomic and chromosome-specific BAC libraries from hexaploid wheat

    Czech Academy of Sciences Publication Activity Database

    Chalhoub, B.; Šafář, Jan; Lefévre, A.; Rouault, P.; Pateyron, S.; Bellec, A.; Janda, Jaroslav; Faivre-Rampant, P.; Doležel, Jaroslav; Caboche, M.

    Califoria, 2003. s. -. [Plant and Animal Genome /11./. 11.01.2003-15.01.2003, San Diego] Institutional research plan: CEZ:AV0Z5038910 Keywords : Triticum aestivum L. * BAC librares * flow-assisted chromosome sorting Subject RIV: EB - Genetics ; Molecular Biology

  12. Library resource sharing in British Columbia post-secondary libraries

    OpenAIRE

    Stuart-Stubbs, Basil

    1992-01-01

    Objective of Study, from the Executive Summary: To identify and evaluate the key components that facilitate or restrict resource sharing of library collections and other information resources among B.C. libraries. A primary goal is to determine what can be done to extend resource sharing by removing barriers and improving those elements that affect the quantity and quality of services. Information is to be collected about current policies and practices, cost and related financial require...

  13. Human Resource Development in Hybrid Libraries

    OpenAIRE

    Prakasan, E. R.; Swarna, T.; Vijai Kumar, *

    2000-01-01

    This paper explores the human resources and development implications in hybrid libraries. Due to technological changes in libraries, which is a result of the proliferation of electronic resources, there has been a shift in workloads and workflow, requiring staff with different skills and educational backgrounds. Training of staff at all levels in information technology is the key to manage change, alleviate anxiety in the workplace and assure quality service in the libraries. Staff developmen...

  14. Construction of a bacterial artificial chromosome (BAC) library of Lycopersicon esculentum cv. Stevens and its application to physically map the Sw-5 locus

    NARCIS (Netherlands)

    Spassova, MI; Prins, M; Stevens, MR; Hille, J; Goldbach, RW; Spassova, Mariana I.; Stevens, Mikel R.; Goldbach, Rob W.

    1999-01-01

    The Sw-5 gene is a dominantly inherited resistance gene in tomato and functional against a number of tospovirus species. The gene has been mapped on chromosome 9, tightly linked to RFLP markers CT220 and SCAR421. To analyse the Sw-5 locus, a BAC genomic library was constructed of tomato cv. Stevens,

  15. Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii: a new resource for plant comparative genomics

    Directory of Open Access Journals (Sweden)

    Chapple Clint

    2005-06-01

    Full Text Available Abstract Background The lycophytes are an ancient lineage of vascular plants that diverged from the seed plant lineage about 400 Myr ago. Although the lycophytes occupy an important phylogenetic position for understanding the evolution of plants and their genomes, no genomic resources exist for this group of plants. Results Here we describe the construction of a large-insert bacterial artificial chromosome (BAC library from the lycophyte Selaginella moellendorffii. Based on cell flow cytometry, this species has the smallest genome size among the different lycophytes tested, including Huperzia lucidula, Diphaiastrum digita, Isoetes engelmanii and S. kraussiana. The arrayed BAC library consists of 9126 clones; the average insert size is estimated to be 122 kb. Inserts of chloroplast origin account for 2.3% of the clones. The BAC library contains an estimated ten genome-equivalents based on DNA hybridizations using five single-copy and two duplicated S. moellendorffii genes as probes. Conclusion The S. moellenforffii BAC library, the first to be constructed from a lycophyte, will be useful to the scientific community as a resource for comparative plant genomics and evolution.

  16. Managing Academic Libraries with Fewer Resources.

    Science.gov (United States)

    Riggs, Donald E.

    1992-01-01

    A discussion of academic library management during retrenchment looks at a variety of issues, including staffing needs in the labor-intensive library environment, acquisitions budgeting, interlibrary cooperation (ownership vs. access to resources), entrepreneurship and strategic planning for problem solving, and use of total quality management…

  17. BAC CLONES GENERATED FROM SHEARED DNA

    OpenAIRE

    Osoegawa, Kazutoyo; Vessere, Gery M.; Shu, Chung Li; Hoskins, Roger A.; Abad, José P.; de Pablos, Beatriz; Villasante, Alfredo; de Jong, Pieter J.

    2006-01-01

    BAC libraries generated from restriction-digested genomic DNA display representational bias and lack some sequences. To facilitate completion of genome projects, procedures have been developed to create BACs from DNA physically sheared to create fragments extending up to 200 kb. The DNA fragments were repaired to create blunt ends and ligated to a new BAC vector. This approach has been tested by generating BAC libraries from Drosophila DNA, with average insert lengths between 50 – 150 kb. The...

  18. Construction of an integrated genetic linkage map for the A genome of Brassica napus using SSR markers derived from sequenced BACs in B. rapa

    OpenAIRE

    Xu, Jinsong; Qian, Xiaoju; Wang, Xiaofeng; Li, Ruiyuan; Cheng, Xiaomao; Yang, Yuan; Fu, Jie; Zhang, Shunchang; King, Graham J; Wu, Jiangsheng; Liu, Kede

    2010-01-01

    Background The Multinational Brassica rapa Genome Sequencing Project (BrGSP) has developed valuable genomic resources, including BAC libraries, BAC-end sequences, genetic and physical maps, and seed BAC sequences for Brassica rapa. An integrated linkage map between the amphidiploid B. napus and diploid B. rapa will facilitate the rapid transfer of these valuable resources from B. rapa to B. napus (Oilseed rape, Canola). Results In this study, we identified over 23,000 simple sequence repeats ...

  19. A five-fold pig bacterial artificial chromosome library:a resource for positional cloning and physical mapping

    Institute of Scientific and Technical Information of China (English)

    LIU Wei; LI Ning; ZHANG Ying; LIU Zhaoliang; GUO Li; WANG Xiaobo; FEI Jing; FENG Jidong; ZHAO Rui; HU Xiaoxiang

    2006-01-01

    A pig BAC library was constructed with genomic DNA from a male Erhualian pig. After partial digestion with Hind Ⅲ or BamH I the fragments obtained were cloned into the pBeloBAC11 vector. The library consists of 184320 clones which stored in 480pieces 384-well plates (20 plates per superpool). A two-step 4-dimension PCR screening system was established to screen the positive clones. An average insert size of 128 kb was estimated from 105 randomly isolated clones, which indicates that the library is more than five times of genomic coverage. For the demonstration of the probability to pick out any unique genes or DNA markers from the library, 10single-copy genes were screened out and the positive clones were yielded between 1 and 8 with an average of 3.6. Positive superpools were obtained for 32 microsatellite markers selected from different regions of pig genome. The number of positive superpools for each marker varies from 1 to 9 with an average of 4.78. This BAC library provides an additional resource for pig physical mapping and gene identification.

  20. Use of electronic information resources in goverment libraries

    OpenAIRE

    Simona Omahen; Maja Žumer

    2003-01-01

    The article presents the use of electronic information resources in government libraries in Slovenia. It starts with the definition of government libraries and electronic publications. On a selected sample of government libraries, the state of the usage of electronic information resources in government libraries was studied. On the basis of interviews, carried out in five government libraries, it was established that government libraries mostly do not focus on, or even think about, the use of...

  1. The first insight into the Salvia (Lamiaceae) genome via BAC library construction and high-throughput sequencing of target BAC clones

    OpenAIRE

    Vautrin, Sonia; Song, Chi; Zhu, Ying Jie; Berges, Hélène; Sun, Chao

    2015-01-01

    Salvia is a representative genus of Lamiaceae, a eudicot family with significant species diversity and population adaptibility. One of the key goals of Salvia genomics research is to identify genes of adaptive significance. This information may help to improve the conservation of adaptive genetic variation and the management of medicinal plants to increase their health and productivity. Large-insert genomic libraries are a fundamental tool for achieving this purpose. We report herein the cons...

  2. Library Resources and Services and Publication Productivity

    OpenAIRE

    Ngah, Z.A.

    2001-01-01

    Present the results of a study that compares the perceived adequacy of library resources for research, the formal channels found to be useful in providing information needed for research, the methods used to keep abreast with current research literature, the problems faced when obtaining information required for research with publication productivity of 83 academic engineers and 239 academic scientists from the University of Malaya and National University of Malaysia. Respondents' views were ...

  3. Collaborative digital library of historical resources: Evaluation of first users

    OpenAIRE

    Abdullah, Abrizah; Zainab, A. N.

    2013-01-01

    This paper describes the digital library of historical resources, a research project which involves building a testbed for the purpose of developing and testing new collaborative digital library functionality and presents an initial analysis of the digital library's public use on the web. The digital library is modeled to focus on serving secondary students information needs in conducting history projects. As such, in the implementation of the digital library, the use of online resources woul...

  4. Identification of an extensive gene cluster among a family of PPOs in Trifolium pratense L. (red clover using a large insert BAC library

    Directory of Open Access Journals (Sweden)

    Thomas Ann

    2009-07-01

    Full Text Available Abstract Background Polyphenol oxidase (PPO activity in plants is a trait with potential economic, agricultural and environmental impact. In relation to the food industry, PPO-induced browning causes unacceptable discolouration in fruit and vegetables: from an agriculture perspective, PPO can protect plants against pathogens and environmental stress, improve ruminant growth by increasing nitrogen absorption and decreasing nitrogen loss to the environment through the animal's urine. The high PPO legume, red clover, has a significant economic and environmental role in sustaining low-input organic and conventional farms. Molecular markers for a range of important agricultural traits are being developed for red clover and improved knowledge of PPO genes and their structure will facilitate molecular breeding. Results A bacterial artificial chromosome (BAC library comprising 26,016 BAC clones with an average 135 Kb insert size, was constructed from Trifolium pratense L. (red clover, a diploid legume with a haploid genome size of 440–637 Mb. Library coverage of 6–8 genome equivalents ensured good representation of genes: the library was screened for polyphenol oxidase (PPO genes. Two single copy PPO genes, PPO4 and PPO5, were identified to add to a family of three, previously reported, paralogous genes (PPO1–PPO3. Multiple PPO1 copies were identified and characterised revealing a subfamily comprising three variants PPO1/2, PPO1/4 and PPO1/5. Six PPO genes clustered within the genome: four separate BAC clones could be assembled onto a predicted 190–510 Kb single BAC contig. Conclusion A PPO gene family in red clover resides as a cluster of at least 6 genes. Three of these genes have high homology, suggesting a more recent evolutionary event. This PPO cluster covers a longer region of the genome than clusters detected in rice or previously reported in tomato. Full-length coding sequences from PPO4, PPO5, PPO1/5 and PPO1/4 will facilitate

  5. Construction of an integrated genetic linkage map for the A genome of Brassica napus using SSR markers derived from sequenced BACs in B. rapa

    OpenAIRE

    King Graham J; Zhang Shunchang; Fu Jie, .; Yang Yuan; Cheng Xiaomao; Li Ruiyuan; Wang Xiaofeng; Qian Xiaoju; Xu Jinsong; Wu Jiangsheng; Liu Kede

    2010-01-01

    Abstract Background The Multinational Brassica rapa Genome Sequencing Project (BrGSP) has developed valuable genomic resources, including BAC libraries, BAC-end sequences, genetic and physical maps, and seed BAC sequences for Brassica rapa. An integrated linkage map between the amphidiploid B. napus and diploid B. rapa will facilitate the rapid transfer of these valuable resources from B. rapa to B. napus (Oilseed rape, Canola). Results In this study, we identified over 23,000 simple sequence...

  6. A Digital Library Resource Web Site: Project DL.

    Science.gov (United States)

    Kochtanek, Thomas R.; Hein, Karen K.; Kassim, Ahmad Rafee Che

    2001-01-01

    Describes Project DL whose goal was to provide a Web site where diverse information sources on digital libraries are available. Discusses the Web site design at the University of Missouri-Columbia that includes three sections: digital library collections, digital library resources, and digital library Web sites. (Author/LRW)

  7. The Library Search Engine: A Smart Solution for Integrating Resources Beyond Library Holdings

    OpenAIRE

    Karin Herm; Sibylle Volz

    2008-01-01

    The Cooperative Library Network Berlin-Brandenburg (KOBV), Germany addresses the problem of how to integrate resources found outside the library and library holdings into a single discovery tool. It presents a solution that uses open source technology to develop a next-generation catalog interface called the Library Search Engine. This pilot project was launched in 2007 with the library of Albert Einstein Science Park, Potsdam. The idea was to design and develop a fast and convenient search t...

  8. Integrating print and digital resources in library collections

    CERN Document Server

    Katz, Linda S

    2013-01-01

    Get informed answers to your questions and concerns about integrating the materials in your library's collectionLibrary collections have always included materials in many formats?handling a mix of material types is an accepted part of library work. And in recent years, the very concept of ?collection? has been significantly redefined by the addition of electronic resources. But are print and digital materials really merged in library collections or are they treated and maintained as separate entities? Integrating Print and Digital Resources in Library Collections examines a variety

  9. Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii: a new resource for plant comparative genomics

    OpenAIRE

    Chapple Clint; Carlson John; Arumuganathan K; Mueller Christopher; Kudrna Dave; Weng Jing-Ke; Kim Hye Ran; Sisneros Nicholas; Luo Meizhong; Tanurdzic Milos; Wang Wenming; de Pamphilis Claude; Mandoli Dina; Tomkins Jeff; Wing Rod A

    2005-01-01

    Abstract Background The lycophytes are an ancient lineage of vascular plants that diverged from the seed plant lineage about 400 Myr ago. Although the lycophytes occupy an important phylogenetic position for understanding the evolution of plants and their genomes, no genomic resources exist for this group of plants. Results Here we describe the construction of a large-insert bacterial artificial chromosome (BAC) library from the lycophyte Selaginella moellendorffii. Based on cell flow cytomet...

  10. Information resources management of T.I.S.S library

    OpenAIRE

    Pothare, R. C.; Subramanian, Shyamala; Angadi, Mallikarjun

    2003-01-01

    Gives step-by-step information resource development, including hardware and software in T.I.S.S. Library. With the changing concept of document and user’s demand, library needs to create indigenous databases and explore external online databases. An attempt has been made to explain information management using IT applications and application software such as CDS/ISIS as well as SLIM ++ (system for library and information management Software). It also highlights the digitization of Library an...

  11. Integrating Electronic Resources into the Library Catalog: A Collaborative Approach.

    Science.gov (United States)

    Herrera, Gail; Aldana, Lynda

    2001-01-01

    Describes a project at the University of Mississippi Libraries to catalog purchased electronic resources so that access to these resources is available only via the Web-based library catalog. Discusses collaboration between cataloging and systems personnel; and describes the MARC catalog record field that contains the information needed to locate…

  12. FILTR: Flash Isotope Library and Training Resource

    International Nuclear Information System (INIS)

    The subject of radiation detection is replete with complex concepts and challenging nomenclature. Furthermore, a daunting variety of radioactive isotopes may be encountered during the routine operation of a radiation detector. Individuals tasked with searching for illicit sources of radiation must remain vigilant while navigating through more frequently encountered mundane and legitimate radioactive sources. The Flash Isotope Library and Training Resource (FILTR) is being developed as an easily accessible and intuitive reference tool to manage the high volume of complex information required for this task. FILTR is an extended version of the Primary Utility for Nuclear Terminology (PUNT) software developed by the Counter Measures Test Beds group at Lawrence Livermore National Laboratory for the United States Secret Service. Authored in the Flash multimedia development environment, FILTR contains detailed information on potentially encountered isotopes as well as training on radiation and operational procedures. Reference material is organized to present critical information quickly while facilitating more in-depth investigation through an intuitive interface and engaging content. FILTR is being developed for a diverse audience of law enforcement organizations and government agencies and a wide range of skill sets from expert analysts to officers whose primary role is not radiation detection. Additionally, the wide compatibility of Flash content will allow FILTR to be readily accessible through the growing number of multi-media enabled electronic devices, including PDAs and cellular phones

  13. The importance of human resources for school library performance

    Directory of Open Access Journals (Sweden)

    Jadranka Matić-Zupančič

    1997-01-01

    Full Text Available The article presents an insight into the survey of the performance of elementary and high school libraries on the territory of the municipalities of Novo mesto,Šentjernej and Škocjan, starting from the comparison between the actual state of school libraries with standards and norms for school libraries.For this purpose, questionnaires were applied to 16 elementary and 5 high-school libraries.The central part of the article is the analysis of the questionnaires and the assessment of standards whereupon norms for human resources are stressed; the realization of other standards for school libraries largely depends upon the number and expertise of the employees.

  14. Library Support for Resource Constrained Accelerators

    DEFF Research Database (Denmark)

    Brock-Nannestad, Laust; Karlsson, Sven

    2014-01-01

    this paper, we present a lightweight system where an accelerator can remotely execute library functions on a host processor. The implementation takes up 750 bytes but can replace arbitrary library calls leading to significant savings in memory foot print. We evaluate with a set of SPLASH-2 applications...

  15. Multi-registration of software library resources

    Science.gov (United States)

    Archer, Charles J.; Blocksome, Michael A.; Ratterman, Joseph D.; Smith, Brian E.

    2011-04-05

    Data communications, including issuing, by an application program to a high level data communications library, a request for initialization of a data communications service; issuing to a low level data communications library a request for registration of data communications functions; registering the data communications functions, including instantiating a factory object for each of the one or more data communications functions; issuing by the application program an instruction to execute a designated data communications function; issuing, to the low level data communications library, an instruction to execute the designated data communications function, including passing to the low level data communications library a call parameter that identifies a factory object; creating with the identified factory object the data communications object that implements the data communications function according to the protocol; and executing by the low level data communications library the designated data communications function.

  16. Resource sharing in libraries concepts, products, technologies, and trends

    CERN Document Server

    Breeding, Marshall

    2014-01-01

    Supplementing your local collection through resource sharing is a smart way to ensure your library has the resources to satisfy the needs of your users. Marshall Breeding's new Library Technology Report explores technologies and strategies for sharing resources, helping you streamline workflows and improve resource-sharing services by covering key strategies like interlibrary loan, consortial borrowing, document delivery, and shared collections. You'll also learn about such trends and services as:OCLC WorldCat Resource Sharing, and other systems that facilitate cooperative, reciprocal lendingS

  17. Preservation of information resources in libraries : new challenges

    OpenAIRE

    Kademani, B. S.; Kalyane, V. L.; Vijai Kumar, *

    2003-01-01

    This paper discusses the importance and need of preservation of information resources in libraries, factors that affect degradation and deterioration of library materials and methods of tackling them. It also discusses the preservation problems that are associated with the digital information and the challenges faced by the librarians in preserving the intellectual content contained in the digital media.

  18. Resource Provisions of a High School Library Collection

    Science.gov (United States)

    Collins, Karla B.; Doll, Carol A.

    2012-01-01

    The mission of the school library "is to ensure students and staff are effective users of ideas and information" (AASL 2009, 8). The school library collection should, therefore, support instruction throughout the school. However, teachers do not always understand the potential value of the resources available. This research explored perceptions…

  19. American Indian Resource Manual for Public Libraries. Bulletin No. 92429.

    Science.gov (United States)

    de Usabel, Frances; Roeber, Jane A.

    This manual, written primarily for Wisconsin public librarians, is designed to provide practical assistance to those who wish to expand public library collections of American Indian (defined as people in the United States and Canada who trace their lineage to the original habitants of these lands) resources and to improve related library programs…

  20. Human Resource Management in Library and Information Services.

    Science.gov (United States)

    Line, Maurice B.; Kinnell, Margaret

    1993-01-01

    Discussion of human resource management focuses on academic libraries. Topics addressed include the influence of information technology; strategic planning; equal opportunities; recruitment; staff appraisal; quality of working life; motivation; job satisfaction; participative management; leadership; burnout; conflict; organizational structures;…

  1. National Library of Medicine Web Resources for Student Health Professionals

    Energy Technology Data Exchange (ETDEWEB)

    Womble, R.

    2010-04-02

    Familiarize students affiliated with the Student National Medical Association with the National Library of Medicine's online resources that address medical conditions, health disparities, and public health preparedness needs.

  2. Resource discovery in distributed digital libraries through visual knowledge navigation

    Institute of Scientific and Technical Information of China (English)

    GU Qian-yi; AHMAD Faisal; SUMNER Tamara

    2005-01-01

    In order to support users to search and browse for various resources, digital libraries are composed of discovery systems which provide user interface and information retrieve system. Recent researches in Information Retrieval have investigated different techniques through improving precision and recall to enhance the effectiveness of discovery system in digital libraries. In this paper, we present our work to enhance discovery system effectiveness with a different approach, through resource discovery based on visual knowledge navigation. In our Strand Map Services project under National Science Digital Library, we introduce the visual resource discovery system called conceptual browsing interfaces, to help educators and learners to locate,comprehend and use educational resources in digital libraries. The paper begins with a short introduction of the Strand Map Services. Then we illustrate the service architecture, the design and implementation of its major components. We will focus our discussion of how the visualization system of the Strand Map Services supports the visual knowledge navigation for distributed digital libraries. This includes the knowledge acquisition of the conceptual browsing interfaces, different knowledge representations in the system perspective and user interface perspective, visualization system modules, algorithm and Web services integration to use visual knowledge navigation to enhance resource discovery in digital libraries.

  3. Libraries and Resource Centres for Tertiary Education

    OpenAIRE

    OECD

    1998-01-01

    PEB, in co-operation with the Programme on Institutional Management in Higher Education (IMHE), hosted an experts meeting to determine how existing libraries for tertiary education may be adapted and new ones conceived to meet the future needs of the students, institutions and communities concerned.

  4. The development and characterisation of a bacterial artificial chromosome library for Fragaria vesca

    OpenAIRE

    Abbott Albert G; Monfort Amparo; Muñoz-Torres Monica C; Sargent Daniel J; Girona Elena; Bonet Julio; Arús Pere; Simpson David W; Davik Jahn

    2009-01-01

    Abstract Background The cultivated strawberry Fragaria ×ananassa is one of the most economically-important soft-fruit species. Few structural genomic resources have been reported for Fragaria and there exists an urgent need for the development of physical mapping resources for the genus. The first stage in the development of a physical map for Fragaria is the construction and characterisation of a high molecular weight bacterial artificial chromosome (BAC) library. Methods A BAC library, cons...

  5. Why Should I Use University Library Website Resources? Discipline Differences

    Science.gov (United States)

    Kim, Yong-Mi

    2011-01-01

    Users across academic disciplines utilize different information sources based on the resource's usefulness and relevance. This study's findings show that users from arts and sciences disciplines are much more likely to utilize university library website resources and printed materials than business users who heavily rely on commercial websites.…

  6. High throughput direct end sequencing of BAC clones.

    OpenAIRE

    Kelley, J M; Field, C E; Craven, M B; Bocskai, D; Kim, U J; Rounsley, S D; Adams, M D

    1999-01-01

    Libraries constructed in bacterial artificial chromosome (BAC) vectors have become the choice for clone sets in high throughput genomic sequencing projects primarily because of their high stability. BAC libraries have been proposed as a source for minimally over-lapping clones for sequencing large genomic regions, and the use of BAC end sequences (i.e. sequences adjoining the insert sites) has been proposed as a primary means for selecting minimally overlapping clones for sequencing large gen...

  7. The National Resource Library for Science and Technology in Sweden: A Nordic model of cooperating technology libraries

    Science.gov (United States)

    Lagar, Gunnar

    1994-01-01

    The scope of this presentation is to give a state-of-the-art report on the present situation of Nordic technology libraries, to elaborate on a plan for national resource libraries in Sweden, and to share how the Royal Institute of Technology Library in Stockholm (KTHB) has fostered a network of cooperating libraries in order to optimize government funding for the system of resource libraries.

  8. Euler European Libraries and Electronic Resources in Mathematical Sciences

    CERN Document Server

    The Euler Project. Karlsruhe

    The European Libraries and Electronic Resources (EULER) Project in Mathematical Sciences provides the EulerService site for searching out "mathematical resources such as books, pre-prints, web-pages, abstracts, proceedings, serials, technical reports preprints) and NetLab (for Internet resources), this outstanding engine is capable of simple, full, and refined searches. It also offers a browse option, which responds to entries in the author, keyword, and title fields. Further information about the Project is provided at the EULER homepage.

  9. Utilization of Super BAC Pools and Fluidigm Access Array Platform for High-Throughput BAC Clone Identification: Proof of Concept

    OpenAIRE

    Peter J Maughan; Smith, Scott M.; Joshua A. Raney

    2012-01-01

    Bacterial artificial chromosome (BAC) libraries are critical for identifying full-length genomic sequences, correlating genetic and physical maps, and comparative genomics. Here we describe the utilization of the Fluidigm access array genotyping system in conjunction with KASPar genotyping technology to identify individual BAC clones corresponding to specific single-nucleotide polymorphisms (SNPs) from an Amplicon Express seven-plate super pooled Amaranthus hypochondriacus BAC library. Ninety...

  10. Optimal Resource Allocation in Library Systems

    Science.gov (United States)

    Rouse, William B.

    1975-01-01

    Queueing theory is used to model processes as either waiting or balking processes. The optimal allocation of resources to these processes is defined as that which maximizes the expected value of the decision-maker's utility function. (Author)

  11. Distribution of Genes and Repetitive Elements in the Diabrotica virgifera virgifera Genome Estimated Using BAC Sequencing

    Directory of Open Access Journals (Sweden)

    Brad S. Coates

    2012-01-01

    Full Text Available Feeding damage caused by the western corn rootworm, Diabrotica virgifera virgifera, is destructive to corn plants in North America and Europe where control remains challenging due to evolution of resistance to chemical and transgenic toxins. A BAC library, DvvBAC1, containing 109,486 clones with 104±34.5 kb inserts was created, which has an ~4.56X genome coverage based upon a 2.58 Gb (2.80 pg flow cytometry-estimated haploid genome size. Paired end sequencing of 1037 BAC inserts produced 1.17 Mb of data (~0.05% genome coverage and indicated ~9.4 and 16.0% of reads encode, respectively, endogenous genes and transposable elements (TEs. Sequencing genes within BAC full inserts demonstrated that TE densities are high within intergenic and intron regions and contribute to the increased gene size. Comparison of homologous genome regions cloned within different BAC clones indicated that TE movement may cause haplotype variation within the inbred strain. The data presented here indicate that the D. virgifera virgifera genome is large in size and contains a high proportion of repetitive sequence. These BAC sequencing methods that are applicable for characterization of genomes prior to sequencing may likely be valuable resources for genome annotation as well as scaffolding.

  12. Open Education Resources: The New Paradigm in Academic Libraries

    Directory of Open Access Journals (Sweden)

    Carmen Mitchell

    2014-04-01

    Full Text Available Academic library budgets are contracting while library usage is increasing. How can academic libraries best help campuses reduce costs and better serve their communities? One strategy is collecting campus-created content online and making it available through the campus Institutional Repository. All faculty, including tenure track and adjuncts, at California State University San Marcos were invited to participate in a brief Web-based survey with both quantitative and qualitative questions. With an 18% response rate, the survey results indicate a strong interest in free or reduced cost educational materials, as well as a high level of concern about the cost of educational materials. Faculty responses indicate they are looking for alternatives to high priced curriculum materials, and are looking to the library for assistance. The crisis in scholarly communication and educational budgets is coming together to create a surge of support for free or low cost educational resources. Many campuses across the country have created programs to support open educational resources, with the main push coming from campus libraries or librarians. The data from this survey and examination of current campus climate, combined with the analysis of implementation factors by other organizations, will bolster the argument for libraries to create open repositories for campus scholarship.

  13. Shaping the Curriculum: The Power of a Library's Digital Resources

    Science.gov (United States)

    Kirkwood, Patricia

    2011-01-01

    Researchers were the first adopters of digital resources available through the library. Online journals and databases make finding research articles much easier than when this author started as a librarian more than 20 years ago. Speedier interlibrary loan due to digital delivery means research materials are never far away. Making it easier for…

  14. Human BAC Ends

    OpenAIRE

    Zhao, Shaying

    2000-01-01

    The Human BAC Ends database includes all non-redundant human BAC end sequences (BESs) generated by The Institute for Genomic Research (TIGR), the University of Washington (UW) and California Institute of Technology (CalTech). It incorporates the available BAC mapping data from different genome centers and the annotation results of each end sequence for the contents of repeats, ESTs and STS markers. For each BAC end the database integrates the sequence, the phred quality scores, the map and th...

  15. Construction of an integrated genetic linkage map for the A genome of Brassica napus using SSR markers derived from sequenced BACs in B. rapa

    Directory of Open Access Journals (Sweden)

    King Graham J

    2010-10-01

    Full Text Available Abstract Background The Multinational Brassica rapa Genome Sequencing Project (BrGSP has developed valuable genomic resources, including BAC libraries, BAC-end sequences, genetic and physical maps, and seed BAC sequences for Brassica rapa. An integrated linkage map between the amphidiploid B. napus and diploid B. rapa will facilitate the rapid transfer of these valuable resources from B. rapa to B. napus (Oilseed rape, Canola. Results In this study, we identified over 23,000 simple sequence repeats (SSRs from 536 sequenced BACs. 890 SSR markers (designated as BrGMS were developed and used for the construction of an integrated linkage map for the A genome in B. rapa and B. napus. Two hundred and nineteen BrGMS markers were integrated to an existing B. napus linkage map (BnaNZDH. Among these mapped BrGMS markers, 168 were only distributed on the A genome linkage groups (LGs, 18 distrubuted both on the A and C genome LGs, and 33 only distributed on the C genome LGs. Most of the A genome LGs in B. napus were collinear with the homoeologous LGs in B. rapa, although minor inversions or rearrangements occurred on A2 and A9. The mapping of these BAC-specific SSR markers enabled assignment of 161 sequenced B. rapa BACs, as well as the associated BAC contigs to the A genome LGs of B. napus. Conclusion The genetic mapping of SSR markers derived from sequenced BACs in B. rapa enabled direct links to be established between the B. napus linkage map and a B. rapa physical map, and thus the assignment of B. rapa BACs and the associated BAC contigs to the B. napus linkage map. This integrated genetic linkage map will facilitate exploitation of the B. rapa annotated genomic resources for gene tagging and map-based cloning in B. napus, and for comparative analysis of the A genome within Brassica species.

  16. Human resource management and career planning in a larger library

    Directory of Open Access Journals (Sweden)

    Jelka Gazvoda

    1997-01-01

    Full Text Available Human resource management is presented as a managerial function which is used to develop potential abilities of the employees to achieve organizational goals.Different perception of the employees is essential - people working in the organization are treated as capital and not as an expenditure. In human resource management the most important view of the employees is their potential growth and professional development, training for acquiring new responsibilities and encouragement for innovation. Library management is becoming more and more complex as the result of introducing new technologies. For this reason libraries need well trained people with potentials to modernize library performance and to overcome the conflict between the traditional organizational culture and the requirements of the modem technologically developed environment. The author presents different techniques of active human resource management, which can be used in larger libraries where an appropriate number of employees exists to realize different programmes with. These are programmes for education, staffing,career planning, stimmulation and reward systems, job redefinition and enrichment,and other forms of internal segmentation.

  17. A hyperactive piggyBac transposase for mammalian applications

    OpenAIRE

    Yusa, K; Zhou, L.; Li, M. A.; Bradley, A; Craig, N. L.

    2011-01-01

    DNA transposons have been widely used for transgenesis and insertional mutagenesis in various organisms. Among the transposons active in mammalian cells, the moth-derived transposon piggyBac is most promising with its highly efficient transposition, large cargo capacity, and precise repair of the donor site. Here we report the generation of a hyperactive piggyBac transposase. The active transposition of piggyBac in multiple organisms allowed us to screen a transposase mutant library in yeast ...

  18. A BAC-based physical map of the Drosophila buzzatii genome

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez, Josefa; Nefedov, Michael; Bosdet, Ian; Casals, Ferran; Calvete, Oriol; Delprat, Alejandra; Shin, Heesun; Chiu, Readman; Mathewson, Carrie; Wye, Natasja; Hoskins, Roger A.; Schein, JacquelineE.; de Jong, Pieter; Ruiz, Alfredo

    2005-03-18

    Large-insert genomic libraries facilitate cloning of large genomic regions, allow the construction of clone-based physical maps and provide useful resources for sequencing entire genomes. Drosophilabuzzatii is a representative species of the repleta group in the Drosophila subgenus, which is being widely used as a model in studies of genome evolution, ecological adaptation and speciation. We constructed a Bacterial Artificial Chromosome (BAC) genomic library of D. buzzatii using the shuttle vector pTARBAC2.1. The library comprises 18,353 clones with an average insert size of 152 kb and a {approx}18X expected representation of the D. buzzatii euchromatic genome. We screened the entire library with six euchromatic gene probes and estimated the actual genome representation to be {approx}23X. In addition, we fingerprinted by restriction digestion and agarose gel electrophoresis a sample of 9,555 clones, and assembled them using Finger Printed Contigs (FPC) software and manual editing into 345 contigs (mean of 26 clones per contig) and 670singletons. Finally, we anchored 181 large contigs (containing 7,788clones) to the D. buzzatii salivary gland polytene chromosomes by in situ hybridization of 427 representative clones. The BAC library and a database with all the information regarding the high coverage BAC-based physical map described in this paper are available to the research community.

  19. Usability Testing for e-Resource Discovery: How Students Find and Choose e-Resources Using Library Web Sites

    Science.gov (United States)

    Fry, Amy; Rich, Linda

    2011-01-01

    In early 2010, library staff at Bowling Green State University (BGSU) in Ohio designed and conducted a usability study of key parts of the library web site, focusing on the web pages generated by the library's electronic resources management system (ERM) that list and describe the library's databases. The goal was to discover how users find and…

  20. RESOURCES CONSUMPTION MANAGEMENT AT THE NATIONAL LIBRARY OF IRAN

    Directory of Open Access Journals (Sweden)

    K. Naddafi, J. Nouri, R. Nabizadeh, N. Shahbod

    2007-07-01

    Full Text Available This paper studies the various resources (water, energy, paper as well as other wastes consumption management at the National Library of Iran in Tehran. After setting the targets and specifying the correct management framework of resources consumption through the green management schedule, the resources analysis in consumption pattern in National Library of Iran was set up. Some attempt plans were performed to find out management solutions to the related issues. For the achievement of this aim, the first step was considered the study of general conditions of the Library through several visits, completing the checklists and questionnaires and then gathering the necessary information and analyzing them. The results of the fuel consumption in transportations related to the National Library showed that the major pollutants produced by the transportation were CO2, CO, NOx, and hydrocarbons. The rate of CO2 had a maximum level of 904 Kg and CO was 6 Kg, NOx was 2 Kg and hydrocarbons were 0.58 Kg. Among the equipments, which use energy in the Library, refrigerators produce the levels of CO2, SO2 and NOx .The total level of CO2, SO2 and NOx produced by all the equipments were 1140.32 Kg, 3.9072 kg and 2.886 Kg respectively. The results of water investigations showed that the total water consumption was 45459 L/year and the greater part of it was related to irrigation of the garden in the courtyard which was equal to 32471 L/year. Regarding to the wastes, the results of 30 times sampling showed the highest level to be related to aluminum and paper and a computer analysis revealed these results in the rates of 19745 Kg/year of CO2, 77.54 Kg/year, SO2 57.4Kg/year NOx and particulate matter was 2.92 Kg/year. Following the investigations carried out in management, executive solutions were suggested which led to the conclusion that the Green Management System should be established at this organization which should result in optimum consumption of resources

  1. Development of genomic resources for Citrus clementina: Characterization of three deep-coverage BAC libraries and analysis of 46,000 BAC end sequences

    OpenAIRE

    Talon Manuel; Ollitrault Patrick; Naranjo M Angel; Terol Javier

    2008-01-01

    Abstract Background Citrus species constitute one of the major tree fruit crops of the subtropical regions with great economic importance. However, their peculiar reproductive characteristics, low genetic diversity and the long-term nature of tree breeding mostly impair citrus variety improvement. In woody plants, genomic science holds promise of improvements and in the Citrus genera the development of genomic tools may be crucial for further crop improvements. In this work we report the char...

  2. Sequencing,annotation and comparative analysis of nine BACs of the giant panda(Ailuropoda melanoleuca)

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    A 10-fold BAC library for the giant panda was constructed and nine BACs were selected to generate finish sequences.These BACs could be used as a validation resource for the de novo assembly accuracy of the whole genome shotgun sequencing reads of the giant panda newly generated by Illumina GA sequencing technology.Complete Sanger sequencing,assembly,annotation and comparative analysis were carried out on the selected BACs of a joint length 878 kb.Homologue search and de novo prediction methods were used to annotate genes and repeats.Twelve protein coding genes were predicted,seven of which could be functionally annotated.The seven genes have an average gene size of about 41 kb,an average coding size of about 1.2 kb and an average exon number of 6 per gene.Besides,seven tRNA genes were found.About 27 percent of the BAC sequence is composed of repeats.A phylogenetic tree was constructed using a neighbor-join algorithm across five species,including the giant panda,human,dog,cat and mouse,which reconfirms dog as the most closely related species to the giant panda.Our results provide detailed sequence and structure information for new genes and repeats of the giant panda,which will be helpful for further studies about the giant panda.

  3. AWARENESS AND USE OF DIGITAL RESOURCES AND SERVICES IN THE IIT DELHI LIBRARY

    OpenAIRE

    Dr. Javed Khan

    2016-01-01

    Information technology (IT) has thrown a new challenge to the libraries. The technology has shown a great impact on the services of the libraries. Libraries use IT for better services and satisfying diverse user needs. Libraries have transformed into digital and virtual libraries where books, journals, and magazines have changed into e-books, e-journals, and e-zines. This has increased the global dissemination of information. Electronic resources (e-resources) are easily accessible in the rem...

  4. Digital Scholarship and Resource Sharing Among Astronomy Libraries: A Case Study of RRI Library

    Science.gov (United States)

    Benegal, V.

    2012-08-01

    Prior to developing consortia, astronomy libraries in India were in an embryonic stage with meager resources and dwindling budgets. It was extremely difficult for them to respond to the needs of their users. Librarians at the various Indian astronomy institutes were forced to look at alternate strategies. Raman Research Institute in Bangalore will be examined in a case study where they attempt to implement resource sharing with other institutes in India and how they were able to provide efficient service to the astronomy community.

  5. Construction and Preliminary Characterization Analysis of Wuzhishan Miniature Pig Bacterial Artificial Chromosome Library with Approximately 8-Fold Genome Equivalent Coverage

    Directory of Open Access Journals (Sweden)

    Changqing Liu

    2013-01-01

    Full Text Available Bacterial artificial chromosome (BAC libraries have been invaluable tools for the genome-wide genetic dissection of complex organisms. Here, we report the construction and characterization of a high-redundancy BAC library from a very valuable pig breed in China, Wuzhishan miniature pig (Sus scrofa, using its blood cells and fibroblasts, respectively. The library contains approximately 153,600 clones ordered in 40 superpools of 10 × 384-deep well microplates. The average insert size of BAC clones was estimated to be 152.3 kb, representing approximately 7.68 genome equivalents of the porcine haploid genome and a 99.93% statistical probability of obtaining at least one clone containing a unique DNA sequence in the library. 19 pairs of microsatellite marker primers covering porcine chromosomes were used for screening the BAC library, which showed that each of these markers was positive in the library; the positive clone number was 2 to 9, and the average number was 7.89, which was consistent with 7.68-fold coverage of the porcine genome. And there were no significant differences of genomic BAC library from blood cells and fibroblast cells. Therefore, we identified 19 microsatellite markers that could potentially be used as genetic markers. As a result, this BAC library will serve as a valuable resource for gene identification, physical mapping, and comparative genomics and large-scale genome sequencing in the porcine.

  6. BacMet

    DEFF Research Database (Denmark)

    Pal, Chandan; Bengtsson-Palme, Johan; Rensing, Christopher Günther T;

    2014-01-01

    scientific literature. The BacMet database contains 470 experimentally verified resistance genes. In addition, the database also contains 25 477 potential resistance genes collected from public sequence repositories. All resistance genes in the BacMet database have been organized according to their molecular...

  7. Toward a Molecular Cytogenetic Map for Cultivated Sunflower (Helianthus annuus L.) by Landed BAC/BIBAC Clones

    Science.gov (United States)

    Feng, Jiuhuan; Liu, Zhao; Cai, Xiwen; Jan, Chao-Chien

    2013-01-01

    Conventional karyotypes and various genetic linkage maps have been established in sunflower (Helianthus annuus L., 2n = 34). However, the relationship between linkage groups and individual chromosomes of sunflower remains unknown and has considerable relevance for the sunflower research community. Recently, a set of linkage group-specific bacterial /binary bacterial artificial chromosome (BAC/BIBAC) clones was identified from two complementary BAC and BIBAC libraries constructed for cultivated sunflower cv. HA89. In the present study, we used these linkage group-specific clones (∼100 kb in size) as probes to in situ hybridize to HA89 mitotic chromosomes at metaphase using the BAC- fluorescence in situ hybridization (FISH) technique. Because a characteristic of the sunflower genome is the abundance of repetitive DNA sequences, a high ratio of blocking DNA to probe DNA was applied to hybridization reactions to minimize the background noise. As a result, all sunflower chromosomes were anchored by one or two BAC/BIBAC clones with specific FISH signals. FISH analysis based on tandem repetitive sequences, such as rRNA genes, has been previously reported; however, the BAC-FISH technique developed here using restriction fragment length polymorphism (RFLP)−derived BAC/BIBAC clones as probes to apply genome-wide analysis is new for sunflower. As chromosome-specific cytogenetic markers, the selected BAC/BIBAC clones that encompass the 17 linkage groups provide a valuable tool for identifying sunflower cytogenetic stocks (such as trisomics) and tracking alien chromosomes in interspecific crosses. This work also demonstrates the potential of using a large-insert DNA library for the development of molecular cytogenetic resources. PMID:23316437

  8. The development and characterisation of a bacterial artificial chromosome library for Fragaria vesca

    Directory of Open Access Journals (Sweden)

    Abbott Albert G

    2009-09-01

    Full Text Available Abstract Background The cultivated strawberry Fragaria ×ananassa is one of the most economically-important soft-fruit species. Few structural genomic resources have been reported for Fragaria and there exists an urgent need for the development of physical mapping resources for the genus. The first stage in the development of a physical map for Fragaria is the construction and characterisation of a high molecular weight bacterial artificial chromosome (BAC library. Methods A BAC library, consisting of 18,432 clones was constructed from Fragaria vesca f. semperflorens accession 'Ali Baba'. BAC DNA from individual library clones was pooled to create a PCR-based screening assay for the library, whereby individual clones could be identified with just 34 PCR reactions. These pools were used to screen the BAC library and anchor individual clones to the diploid Fragaria reference map (FV×FN. Findings Clones from the BAC library developed contained an average insert size of 85 kb, representing over seven genome equivalents. The pools and superpools developed were used to identify a set of BAC clones containing 70 molecular markers previously mapped to the diploid Fragaria FV×FN reference map. The number of positive colonies identified for each marker suggests the library represents between 4× and 10× coverage of the diploid Fragaria genome, which is in accordance with the estimate of library coverage based on average insert size. Conclusion This BAC library will be used for the construction of a physical map for F. vesca and the superpools will permit physical anchoring of molecular markers using PCR.

  9. Current status of "Net Resource subject guide" of Chinese academic libraries

    OpenAIRE

    Qiao, Huan

    2002-01-01

    Chinese Academic Library and Information System (CALIS), one of the most important public service system projects, was authorized and supported financially by the State Council of China. In order to integrate national investment, modern library service and advanced technology, abundant document resources of academic libraries and human resource, CALIS devotes to document guaranteed system construction, with focus on Chinese higher education digital library development. CALIS promotes informat...

  10. A BAC-based physical map of the Hessian fly genome anchored to polytene chromosomes

    Directory of Open Access Journals (Sweden)

    Fellers John P

    2009-07-01

    Full Text Available Abstract Background The Hessian fly (Mayetiola destructor is an important insect pest of wheat. It has tractable genetics, polytene chromosomes, and a small genome (158 Mb. Investigation of the Hessian fly presents excellent opportunities to study plant-insect interactions and the molecular mechanisms underlying genome imprinting and chromosome elimination. A physical map is needed to improve the ability to perform both positional cloning and comparative genomic analyses with the fully sequenced genomes of other dipteran species. Results An FPC-based genome wide physical map of the Hessian fly was constructed and anchored to the insect's polytene chromosomes. Bacterial artificial chromosome (BAC clones corresponding to 12-fold coverage of the Hessian fly genome were fingerprinted, using high information content fingerprinting (HIFC methodology, and end-sequenced. Fluorescence in situ hybridization (FISH co-localized two BAC clones from each of the 196 longest contigs on the polytene chromosomes. An additional 70 contigs were positioned using a single FISH probe. The 266 FISH mapped contigs were evenly distributed and covered 60% of the genome (95,668 kb. The ends of the fingerprinted BACs were then sequenced to develop the capacity to create sequenced tagged site (STS markers on the BACs in the map. Only 3.64% of the BAC-end sequence was composed of transposable elements, helicases, ribosomal repeats, simple sequence repeats, and sequences of low complexity. A relatively large fraction (14.27% of the BES was comprised of multi-copy gene sequences. Nearly 1% of the end sequence was composed of simple sequence repeats (SSRs. Conclusion This physical map provides the foundation for high-resolution genetic mapping, map-based cloning, and assembly of complete genome sequencing data. The results indicate that restriction fragment length heterogeneity in BAC libraries used to construct physical maps lower the length and the depth of the contigs, but is

  11. Genomic tools development for Aquilegia: construction of a BAC-based physical map

    Directory of Open Access Journals (Sweden)

    Hodges Scott A

    2010-11-01

    Full Text Available Abstract Background The genus Aquilegia, consisting of approximately 70 taxa, is a member of the basal eudicot lineage, Ranuculales, which is evolutionarily intermediate between monocots and core eudicots, and represents a relatively unstudied clade in the angiosperm phylogenetic tree that bridges the gap between these two major plant groups. Aquilegia species are closely related and their distribution covers highly diverse habitats. These provide rich resources to better understand the genetic basis of adaptation to different pollinators and habitats that in turn leads to rapid speciation. To gain insights into the genome structure and facilitate gene identification, comparative genomics and whole-genome shotgun sequencing assembly, BAC-based genomics resources are of crucial importance. Results BAC-based genomic resources, including two BAC libraries, a physical map with anchored markers and BAC end sequences, were established from A. formosa. The physical map was composed of a total of 50,155 BAC clones in 832 contigs and 3939 singletons, covering 21X genome equivalents. These contigs spanned a physical length of 689.8 Mb (~2.3X of the genome suggesting the complex heterozygosity of the genome. A set of 197 markers was developed from ESTs induced by drought-stress, or involved in anthocyanin biosynthesis or floral development, and was integrated into the physical map. Among these were 87 genetically mapped markers that anchored 54 contigs, spanning 76.4 Mb (25.5% across the genome. Analysis of a selection of 12,086 BAC end sequences (BESs from the minimal tiling path (MTP allowed a preview of the Aquilegia genome organization, including identification of transposable elements, simple sequence repeats and gene content. Common repetitive elements previously reported in both monocots and core eudicots were identified in Aquilegia suggesting the value of this genome in connecting the two major plant clades. Comparison with sequenced plant genomes

  12. Advanced resources for plant genomics: BAC library specific for the short arm of wheat chromosome 1B

    Czech Academy of Sciences Publication Activity Database

    Janda, Jaroslav; Šafář, Jan; Kubaláková, Marie; Bartoš, Jan; Kovářová, Pavlína; Suchánková, Pavla; Pateyron, S.; Čihalíková, Jarmila; Sourdille, P.; Šimková, Hana; Faivre-Rampant, P.; Hřibová, Eva; Bernard, M.; Lukaszewski, A.; Doležel, Jaroslav; Chalhoub, B.

    2006-01-01

    Roč. 47, - (2006), s. 977-986. ISSN 0960-7412 R&D Projects: GA ČR GA521/04/0607; GA ČR GP521/05/P257; GA ČR GD521/05/H013; GA MŠk LC06004 Institutional research plan: CEZ:AV0Z50380511 Keywords : wheat * genomics * chromosome sorting Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 6.565, year: 2006

  13. Applying Web Usability Techniques to Assess Student Awareness of Library Web Resources

    Science.gov (United States)

    Krueger, Janice; Ray, Ron L.; Knight, Lorrie

    2004-01-01

    The authors adapted Web usability techniques to assess student awareness of their library's Web site. Students performed search tasks using a Web browser. Approaches were categorized according to a student's preference for, and success with, the library's Web resources. Forty-five percent of the students utilized the library's Web site as first…

  14. Hacking Blackboard: Customizing Access to Library Resources through the Blackboard Course Management System

    Science.gov (United States)

    Kellam, Lynda M.; Cox, Richard; Winkler, Hannah

    2009-01-01

    Academic libraries have long been trying to gain access to users through their favorite online spaces, such as social networking sites. In this article a project of the University of North Carolina at Greensboro's University Libraries, which integrated library resources with the campus course management system, Blackboard, is detailed. The main…

  15. Measuring the Value of Library Resources and Student Academic Performance through Relational Datasets

    Directory of Open Access Journals (Sweden)

    Margie Jantti

    2013-06-01

    Full Text Available Objective – This article describes a project undertaken by the University of Wollongong Library (UWL to identify whether a correlation exists between usage of library resources and academic performance.Methods – A multidimensional approach to systems design was implemented, requiring collaboration between among the library, university administration, Performance Indicator Project team (PIP, and information technology services. The project centers on the integration and interrogation of a series of discrete datasets containing student performance, attrition, demographic, borrowing, and electronic resources usage data. PIP built a cube for the library that links usage of library resources to student demographic data and academic performance (the “Library Cube”. Other cubes will be linked later.Results – While initial reports are rudimentary and do not yet incorporate data on e-resource usage, results are favourable in demonstrating the value of using the library information resources in coursework. Based on the data generated to date, students who borrow library resources do outperform students who do not. Early trend data shows up to a 12-point difference in grades.Conclusion – The Library Cube signals a new milestone in the UWL’s quality assessment journey. Well-established measures of effectiveness and efficiency will be further complemented by measures of impact and value, allowing the library to step even closer to the goal of having effective and valued partnerships with the university community to realize teaching, learning, research, and internalization goals.

  16. Performance Evaluation of Karnataka State University Library Resources and Services:A Librarians Approach

    OpenAIRE

    Ramesha; B.D. Kumbar

    2004-01-01

    Library service is the supreme activity to attract the attention of the user community. It is more so in the case of a university library. Looking at the commitment of the university library to promote the cause of healthy higher education, it is essential on the part of the library professionals to evaluate and assesses the usability of the library resources in the light of the vast amount of investment made in acquiring the information resources and the cost of the manpower in managing the ...

  17. The Open Library at AU (Athabasca University): Supporting Open Access and Open Educational Resources

    OpenAIRE

    Colin Elliott; Elaine Fabbro

    2015-01-01

    To address challenges that learners, course creators, librarians and academics involved with OER and MOOCs are facing when looking for scholarly materials, Athabasca University Library has initiated the development of the Open Library at AU. This open library is a full library website that provides easy access to open and free resources. Tools and information literacy tutorials are also included to enable learners, researchers, and others to find, evaluate, and use the information they need f...

  18. Listening to Students: Customer Journey Mapping at Birmingham City University Library and Learning Resources

    Science.gov (United States)

    Andrews, Judith; Eade, Eleanor

    2013-01-01

    Birmingham City University's Library and Learning Resources' strategic aim is to improve student satisfaction. A key element is the achievement of the Customer Excellence Standard. An important component of the standard is the mapping of services to improve quality. Library and Learning Resources has developed a methodology to map these…

  19. End User Resource Valuation in Community College Libraries: A Q Methodology Study

    Science.gov (United States)

    Lucy, Theodore John

    2010-01-01

    The purpose of this study was to explore the valuation process of community college library end-users as they decide which information resources to use when conducting research. This study was designed as an exploratory study using Q methodology and focused on five specific information resources that community college library end-users routinely…

  20. Re-engineering the management of human resources in university libraries

    OpenAIRE

    Francis, A. T.; Humayoon Kabir, S.

    2008-01-01

    Information and Communication Technology application in university libraries poses several issues to be addressed, including those related to the management of human resources. As part of a study on the technology management of university libraries in Kerala, the aspect of human resources was also evaluated. A radical re-design in the prevailing system of management is advocated by the study. As a key component in the proper application of modern technologies in university libraries, human re...

  1. Access to Library Resources for Clinical/Adjunct Faculty: A License-Driven Model

    OpenAIRE

    David Brennan

    2015-01-01

    Clinical/adjunct faculty (also referred to as preceptors) present a unique challenge to academic health sciences libraries, often having appointments that are considered to be affiliates rather than employees as well as having access to resources via their own institution's library. This article presents the experience of the George T. Harrell Health Sciences Library, Penn State Hershey, in providing access to resources for this user group, including a targeted web presence, licensing, and ma...

  2. Resource Sharing in Research Libraries of Latin America

    OpenAIRE

    Lau, Jesús

    1995-01-01

    Research and academic libraries are the most developed information centers in Latin America. Their parent organizations are usually universities, government, and to a less extent private companies. Their library collections are therefore among the best. However, due to several factors, library collaboration and cooperation is limited, especially due to poor telecommunication links. Collections built by research and academic libraries evolve in isolation from one another, a factor that red...

  3. Users' Satisfaction with Library Information Resources and Services: A Case Study College of Health Sciences Library Niger Delta University, Amassoma, Nigeria

    Science.gov (United States)

    Tiemo, Pereware Aghwotu; Ateboh, Benedict Alaowei

    2016-01-01

    This study investigated users' satisfaction with library information resources and services at the College of Health Sciences (CHS) library Niger Delta University, Nigeria. The objective was to determine the level of users satisfaction with library information resources and services. 2 (two) research questions were formulated to guide the study.…

  4. Utilization of Super BAC Pools and Fluidigm Access Array Platform for High-Throughput BAC Clone Identification: Proof of Concept

    Directory of Open Access Journals (Sweden)

    Peter J. Maughan

    2012-01-01

    Full Text Available Bacterial artificial chromosome (BAC libraries are critical for identifying full-length genomic sequences, correlating genetic and physical maps, and comparative genomics. Here we describe the utilization of the Fluidigm access array genotyping system in conjunction with KASPar genotyping technology to identify individual BAC clones corresponding to specific single-nucleotide polymorphisms (SNPs from an Amplicon Express seven-plate super pooled Amaranthus hypochondriacus BAC library. Ninety-six SNP loci, spanning the length of A. hypochondriacus linkage groups 1, 2, and 15, were simultaneously tested for clone identification from four BAC super pools, corresponding to 28 384-well plates, using a single Fluidigm integrated fluidic chip (IFC. Forty-six percent of the SNPs were associated with a single unambiguous identified BAC clone. PCR amplification and next-generation sequencing of individual BAC clones confirmed the IFC clone identification. Utilization of the Fluidigm Dynamic array platform allowed for the simultaneous PCR screening of 10,752 BAC pools for 96 SNP tag sites in less than three hours at a cost of ~$0.05 per reaction.

  5. Performance Evaluation of Karnataka State University Library Resources and Services:A Librarians Approach

    Directory of Open Access Journals (Sweden)

    Ramesha

    2004-03-01

    Full Text Available Library service is the supreme activity to attract the attention of the user community. It is more so in the case of a university library. Looking at the commitment of the university library to promote the cause of healthy higher education, it is essential on the part of the library professionals to evaluate and assesses the usability of the library resources in the light of the vast amount of investment made in acquiring the information resources and the cost of the manpower in managing the library activity. This paper reveals one such attempt with reference to the university libraries in the state of Karnataka which is a part of Independent India.

  6. Rapid construction of a Bacterial Artificial Chromosomal (BAC) expression vector using designer DNA fragments.

    Science.gov (United States)

    Chen, Chao; Zhao, Xinqing; Jin, Yingyu; Zhao, Zongbao Kent; Suh, Joo-Won

    2014-11-01

    Bacterial artificial chromosomal (BAC) vectors are increasingly being used in cloning large DNA fragments containing complex biosynthetic pathways to facilitate heterologous production of microbial metabolites for drug development. To express inserted genes using Streptomyces species as the production hosts, an integration expression cassette is required to be inserted into the BAC vector, which includes genetic elements encoding a phage-specific attachment site, an integrase, an origin of transfer, a selection marker and a promoter. Due to the large sizes of DNA inserted into the BAC vectors, it is normally inefficient and time-consuming to assemble these fragments by routine PCR amplifications and restriction-ligations. Here we present a rapid method to insert fragments to construct BAC-based expression vectors. A DNA fragment of about 130 bp was designed, which contains upstream and downstream homologous sequences of both BAC vector and pIB139 plasmid carrying the whole integration expression cassette. In-Fusion cloning was performed using the designer DNA fragment to modify pIB139, followed by λ-RED-mediated recombination to obtain the BAC-based expression vector. We demonstrated the effectiveness of this method by rapid construction of a BAC-based expression vector with an insert of about 120 kb that contains the entire gene cluster for biosynthesis of immunosuppressant FK506. The empty BAC-based expression vector constructed in this study can be conveniently used for construction of BAC libraries using either microbial pure culture or environmental DNA, and the selected BAC clones can be directly used for heterologous expression. Alternatively, if a BAC library has already been constructed using a commercial BAC vector, the selected BAC vectors can be manipulated using the method described here to get the BAC-based expression vectors with desired gene clusters for heterologous expression. The rapid construction of a BAC-based expression vector facilitates

  7. A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni

    Directory of Open Access Journals (Sweden)

    Coen M Adema

    2006-10-01

    Full Text Available To provide a novel resource for analysis of the genome of Biomphalaria glabrata, members of the international Biomphalaria glabrata Genome Initiative (biology.unm.edu/biomphalaria-genome.html, working with the Arizona Genomics Institute (AGI and supported by the National Human Genome Research Institute (NHGRI, produced a high quality bacterial artificial chromosome (BAC library. The BB02 strain B. glabrata, a field isolate (Belo Horizonte, Minas Gerais, Brasil that is susceptible to several strains of Schistosoma mansoni, was selfed for two generations to reduce haplotype diversity in the offspring. High molecular weight DNA was isolated from ovotestes of 40 snails, partially digested with HindIII, and ligated into pAGIBAC1 vector. The resulting B. glabrata BAC library (BG_BBa consists of 61824 clones (136.3 kb average insert size and provides 9.05 × coverage of the 931 Mb genome. Probing with single/low copy number genes from B. glabrata and fingerprinting of selected BAC clones indicated that the BAC library sufficiently represents the gene complement. BAC end sequence data (514 reads, 299860 nt indicated that the genome of B. glabrata contains ~ 63% AT, and disclosed several novel genes, transposable elements, and groups of high frequency sequence elements. This BG_BBa BAC library, available from AGI at cost to the research community, gains in relevance because BB02 strain B. glabrata is targeted whole genome sequencing by NHGRI.

  8. The Best Cataloger is a Frustrated Library User: Cataloging Failure and the Underutilization of Library Resources

    Directory of Open Access Journals (Sweden)

    John W. Thompson

    2015-08-01

    Full Text Available This essay points out that inconsistencies in the assignment of subject headings and call number can lead to failure to retrieve relevant materials from our libraries. Today it is frequently asserted that bibliographic records cataloged by the Library of Congress or other approved libraries will not require review or editing in our local libraries. This paper provides clear, but by no means unique examples of “cataloging failure” and explains the implications of a policy to add unedited bibliographic records (from vendors such as OCLC to our library catalogs. The result is the omission of otherwise relevant titles from fairly routine searches.

  9. An Evaluation of the Accessibility of E-resources from Theological Library Websites

    Directory of Open Access Journals (Sweden)

    Kate L. Ganski

    2008-06-01

    Full Text Available This study examines the accessibility of e-resources from the websites of theological libraries to discover if theological libraries are providing digital access to e-journals, recommended religious Internet resources, and digital libraries. Qualitative content analysis was used to evaluate the placement and terminology of e-resources on twenty-five theological library websites of faith-based institutions with primarily graduate/professional students. Findings revealed slightly more than half of these websites make e-resources available and are easily accessible through a quick navigational pathway. Terminology used was found to be diverse and varied. These findings suggest that theological library websites are attempting to meet the digital needs of their students. Further study is recommended to understand the impact, if any, these findings may have on the digital needs of theological students.

  10. BAC LIBRARY SCREENING OF PORCINE MILK PROTEIN GENES%利用细菌人工染色体文库(BAC)筛选猪乳蛋白基因

    Institute of Scientific and Technical Information of China (English)

    赵兴波; 铃木恒平; 李宁; 吴常信

    2001-01-01

    Gene clones of porcine Whey Acidic Protein(WAP),α s1-casein,α s2-casein,β-casein and κ-casein were selected and picked up using porcine bacteria artificial chromosome library (pBAC)and its two step-four dimensional screening system,and then discussed the one step-five dimensional screening system of pBAC library.%利用猪细菌人工染色体文库(porcine Bacteria Artificial Chromosome,pBAC)的两步-四维PCR(two step-4-D PCR)方法筛选猪乳清酸性蛋白(Whey Acidic Protein,WAP),αsl-酪蛋白,αs2-酪蛋白,β-酪蛋白,κ-酪蛋白的基因pBAC克隆,并提出了BAC基因文库一步-五维PCR(one step-5-D PCR)筛选基因的方法。

  11. Open Education Resources: The New Paradigm in Academic Libraries

    OpenAIRE

    Carmen Mitchell; Melanie Chu

    2014-01-01

    Academic library budgets are contracting while library usage is increasing. How can academic libraries best help campuses reduce costs and better serve their communities? One strategy is collecting campus-created content online and making it available through the campus Institutional Repository. All faculty, including tenure track and adjuncts, at California State University San Marcos were invited to participate in a brief Web-based survey with both quantitative and qualitative questions. Wi...

  12. Legal Resources Information System for Information Agencies of Specialized Libraries

    CERN Document Server

    Nguyen, Phuc V

    2011-01-01

    In recent years, the rapid development of information technology and communication has a strong impact to industry information - the library. The mission of the industry when in fact the great social place to see the library as knowledge management. Vietnam is in the process of building the rule of law socialist orientation and improves the legal system. So in the current development process, the law library plays an important role in the retention, dissemination and provision of legal information service of legislative, executive and judiciary, particularly especially research, teaching and learning of law school. But the response of the legal information library information agencies remains limited compared to the increasing demand of users.

  13. Quality control of the sheep bacterial artificial chromosome library, CHORI-243

    Directory of Open Access Journals (Sweden)

    Kirkness Ewen F

    2010-12-01

    Full Text Available Abstract Background The sheep CHORI-243 bacterial artificial chromosome (BAC library is being used in the construction of the virtual sheep genome, the sequencing and construction of the actual sheep genome assembly and as a source of DNA for regions of the genome of biological interest. The objective of our study is to assess the integrity of the clones and plates which make up the CHORI-243 library using the virtual sheep genome. Findings A series of analyses were undertaken based on the mapping the sheep BAC-end sequences (BESs to the virtual sheep genome. Overall, very few plate specific biases were identified, with only three of the 528 plates in the library significantly affected. The analysis of the number of tail-to-tail (concordant BACs on the plates identified a number of plates with lower than average numbers of such BACs. For plates 198 and 213 a partial swap of the BESs determined with one of the two primers appear to have occurred. A third plate, 341, also with a significant deficit in tail-to-tail BACs, appeared to contain a substantial number of sequences determined from contaminating eubacterial 16 S rRNA DNA. Additionally a small number of eubacterial 16 S rRNA DNA sequences were present on two other plates, 111 and 338, in the library. Conclusions The comparative genomic approach can be used to assess BAC library integrity in the absence of fingerprinting. The sequences of the sheep CHORI-243 library BACs have high integrity, especially with the corrections detailed above. The library represents a high quality resource for use by the sheep genomics community.

  14. SmartOrBAC

    Directory of Open Access Journals (Sweden)

    Imane BOUIJ-PASQUIER

    2015-11-01

    Full Text Available The emergence of the Internet of Things (IoT paradigm, provides a huge scope for more streamlined living through an increase of smart services but this coincides with an increase in security and privacy concerns, therefore access control has been an important factor in the development of IoT. This work proposes an authorization access model called SmartOrBAC built around a set of security and performance requirements. This model enhances the existing OrBAC (Organization-based Access Control model and adapts it to IoT environments. SmartOrBAC separates the problem into different functional layers and then distributes processing costs between constrained devices and less constrained ones and at the same time addresses the collaborative aspect with a specific solution. This paper also presents the application of SmartOrBAC on a real example of IoT and gives a complexity study demonstrating that even though this model is extensive, it does not add additional complexity regarding traditional access control models.

  15. To get or not to get: the KAUST library e-resources acquisition experience

    KAUST Repository

    Ramli, Rindra M.

    2014-06-01

    In the challenging times of budget cuts and reviews, libraries are faced with issues, among others, such as justifying acquisition, negotiating deals and reviewing current subscriptions (pertaining to electronic resources). With the rapid increase in growth of electronic resources, libraries have to continuously assess their acquisition models and policies to constantly ensure that they are balancing their budget and users’ needs as well. This paper highlights the role played by Technical and IT department of King Abdullah University of Science and Technology (KAUST) library (Saudi Arabia) in acquiring the electronic resources (electronic books, electronic journals, databases, image and reference) for the community. It will describe the processes during the early days when KAUST library was inaugurated; how electronic resources were acquired and what went through during those days. The paper will elaborate further how the acquisition model has evolved and the various important roles played by the library staff in ensuring that acquisitions/subscriptions are justified, within the budget and provides ROI for the library. King Abdullah University is a graduate research university which opened in September 2009 with its first cohort of 800 graduate students (25% female) taught by 100 faculties. The focus of study and research in the university are: Mathematics and Computer Science, Physical Sciences and Life Sciences. The university library started with 10 staff. The library has a “state-of-the-art learning and information resource center supporting graduate education and advanced scientific research” (KAUST, 2010).

  16. We're All in This Together: Library Faculty and Staff and Their Reporting of Electronic Resource Problems

    Science.gov (United States)

    Foster, Anita; Williams, Sarah C.

    2010-01-01

    Libraries continue to shift budgets toward obtaining more electronic resources. Electronic resources can develop problems at any time when a library offers access. Staff collaboration is vital in ensuring availability to those resources. Partnering with areas in the library that work most closely with patrons can help share the load of the…

  17. Developing as new search engine and browser for libraries to search and organize the World Wide Web library resources

    OpenAIRE

    Sreenivasulu, V.

    2000-01-01

    Internet Granthalaya urges world wide advocates and targets at the task of creating a new search engine and dedicated browseer. Internet Granthalaya may be the ultimate search engine exclusively dedicated for every library use to search and organize the world wide web libary resources

  18. GenMapDB: a database of mapped human BAC clones

    OpenAIRE

    Morley, Michael; Arcaro, Melissa; Burdick, Joshua; Yonescu, Raluca; Reid, Thomas; Kirsch, Ilan R.; Cheung, Vivian G.

    2001-01-01

    GenMapDB (http://genomics.med.upenn.edu/genmapdb) is a repository of human bacterial artificial chromosome (BAC) clones mapped by our laboratory to sequence-tagged site markers. Currently, GenMapDB contains over 3000 mapped clones that span 19 chromosomes, chromosomes 2, 4, 5, 9–22, X and Y. This database provides positional information about human BAC clones from the RPCI-11 human male BAC library. It also contains restriction fragment analysis data and end sequen...

  19. Virtual Library: An Evolution of Online Astronomy and Astrophysics Resources for Astronomers

    Science.gov (United States)

    Rai, V. K.

    2010-10-01

    A research library aware of high-speed reference services is essential for researchers. Using optimum resources, such as databases available on the web to speed up research, the librarian has to be proactive. The librarian should explore all available resources on the web. In the field of astronomy and astrophysics, most of the resources available are free to use. The librarian plays a crucial role in providing information about these resources to the researcher. This paper is a compilation of the most popular resources available on the Internet and provides information about who can use these resources, what these resources are, and how to use these resources.

  20. Enhancing Teaching and Learning: Libraries and Open Educational Resources in the Classroom

    Science.gov (United States)

    Davis, Erin; Cochran, Dory; Fagerheim, Britt; Thoms, Becky

    2016-01-01

    Academic libraries continually adjust services to adapt to the ever-changing landscape in higher education. In response to the broken textbook market, libraries are becoming actively involved in the open educational resources (OER) movement. Although there is not a formal program in place, librarians at Utah State University explored a…

  1. Assessment of Data Management Services at New England Region Resource Libraries

    Directory of Open Access Journals (Sweden)

    Julie Goldman

    2015-07-01

    Full Text Available Objective: To understand how New England medical libraries are addressing scientific research data management and providing services to their communities. Setting: The National Network of Libraries of Medicine, New England Region (NN/LM NER contains 17 Resource Libraries. The University of Massachusetts Medical School serves as the New England Regional Medical Library (RML. Sixteen of the NER Resource Libraries completed this survey. Methods: A 40-question online survey assessed libraries’ services and programs for providing research data management education and support. Libraries shared their current plans and institutional challenges associated with developing data services. Results: This study shows few NER Resource Libraries currently integrate scientific research data management into their services and programs, and highlights the region’s use of resources provided by the NN/LM NER RML at the University of Massachusetts Medical School. Conclusions: Understanding the types of data services being delivered at NER libraries helps to inform the NN/LM NER about the eScience learning needs of New England medical librarians and helps in the planning of professional development programs that foster effective biomedical research data services.

  2. Black History: Kindergarten to Senior 4. A Bibliography of Resources Available from the Library, Instructional Resources Branch.

    Science.gov (United States)

    Manitoba Dept. of Education, Winnipeg. Instructional Resources Branch.

    The materials listed in this bibliography are on display in the Multicultural Educational Resource Collection in Winnipeg, Manitoba, and duplicate copies are available for loan from the library. Each bibliography entry offers the author, title, publication information, and call number for literature and other resources in black history and…

  3. Abadina Media Resource Centre (AMRC): A Case Study in Library Service to Primary Schools.

    Science.gov (United States)

    Ogunsheye, F. Adetowun

    1979-01-01

    Describes the philosophy, objectives, services, and experimental research of the Abadina Media Resource Center, which provides library services, materials, and education to elementary school students and provides research and training facilities for Nigerian teachers and school librarians. (Author/CWM)

  4. Annotation and BAC/PAC localization of nonredundant ESTs from drought-stressed seedlings of an indica rice

    Indian Academy of Sciences (India)

    P. Ravindra Babu; A. Chandra Sekhar; Nagabhushana Ithal; G. Markandeya; Arjula R. Reddy

    2002-04-01

    To decipher the genes associated with drought stress response and to identify novel genes in rice, we utilized 1540 high-quality expressed sequence tags (ESTs) for functional annotation and mapping to rice genomic sequences. These ESTs were generated earlier by 3′-end single-pass sequencing of 2000 cDNA clones from normalized cDNA libraries constructed from drought-stressed seedlings of an indica rice. A rice UniGene set of 1025 transcripts was constructed from this collection through the BLASTN algorithm. Putative functions of 559 nonredundant ESTs were identified by BLAST similarity search against public databases. Putative functions were assigned at a stringency E value of 10-6 in BLASTN and BLASTX algorithms. To understand the gene structure and function further, we have utilized the publicly available finished and unfinished rice BAC/PAC (BAC, bacterial artificial chromosome; PAC, P1 artificial chromosome) sequences for similarity search using the BLASTN algorithm. Further, 603 nonredundant ESTs have been mapped to BAC/PAC clones. BAC clones were assigned by a homology of above 95% identity along 90% of EST sequence length in the aligned region. In all, 700 ESTs showed rice EST hits in GenBank. Of the 325 novel ESTs, 128 were localized to BAC clones. In addition, 127 ESTs with identified putative functions but with no homology in IRGSP (International Rice Genome Sequencing Program) BAC/PAC sequences were mapped to the Chinese WGS (whole genome shotgun contigs) draft sequence of the rice genome. Functional annotation uncovered about a hundred candidate ESTs associated with abiotic stress in rice and Arabidopsis that were previously reported based on microarray analysis and other studies. This study is a major effort in identifying genes associated with drought stress response and will serve as a resource to rice geneticists and molecular biologists.

  5. To Get or Not to Get the Kaust Library E-Resources Acquisition Experience

    OpenAIRE

    Ramli, Rindra M.; Kabli, Ola A.

    2014-01-01

    In the challenging times of budget cuts and reviews, libraries are faced with issues such as justifying acquisition, negotiating deals and reviewing current subscriptions (pertaining to electronic resources) among others. With the rapid growth of electronic resources, libraries have to continuously assess their acquisition models and policies to constantly ensure that they are balancing their budget and users’ needs as well. This paper highlights the role played by Technical and Digital Servi...

  6. An Overview of CSIR Libraries as Knowledge Resource Centres: Issues and Trends

    OpenAIRE

    Verma, R. K.; Jaya Kalra

    2015-01-01

    A survey of CSIR libraries attached to the 38 laboratories and 3 CSIR units has been made with the main objective of reviewing the current status of the same. For this purpose, the data on infrastructure, resources, and the services provided by the libraries were collected using questionnaire in e-form. It is observed that the libraries are at varying levels and status in terms of infrastructural facilities vis-a-vis status of library staff. Keeping in view the manual guidelines issued by the...

  7. Library Electronic Resource Sharing Among Liberal Arts Colleges: ACS Palladian Alliance Project

    Directory of Open Access Journals (Sweden)

    Wenxian Zhang

    1997-03-01

    Full Text Available 無Effective electronic resource sharing is critical to library information services of the 1990s. Explosion of data and increased cost of information force libraries to work together, and technological advancements present the library service profession a platform for resource sharing. The Palladian Alliance Project of the Associated Colleges of the South is designed to provides ACS member institutions an effective means to enhance information access for their faculty and students, and achieve significant cost containment in the years to come.

  8. Making User Rights Clear: Adding e-resource License Information in Library Systems

    Directory of Open Access Journals (Sweden)

    Jenny Jing

    2015-07-01

    Full Text Available Libraries sign a wide variety of licensing agreements that specify terms of both access and use of a publisher’s electronic collections. Adding easily accessible licensing information to collections helps ensure that library users comply with these agreements. This article will describe the addition of licensing permissions to resource displays using Mondo by Queen’s University and Scholars Portal (a service of the Ontario Council of University Libraries. We will give a brief introduction to Mondo and explain how we improved Mondo to add the license permissions to different library systems. The systems we used are an ILS (Voyager, an OpenURL Link Resolver (360 Link, and a Discovery System (Summon. However, libraries can use Mondo to add the license permissions to other library systems which allow user configurations.

  9. BAC end sequencing of Pacific white shrimp Litopenaeus vannamei: a glimpse into the genome of Penaeid shrimp

    Science.gov (United States)

    Zhao, Cui; Zhang, Xiaojun; Liu, Chengzhang; Huan, Pin; Li, Fuhua; Xiang, Jianhai; Huang, Chao

    2012-05-01

    Little is known about the genome of Pacific white shrimp ( Litopenaeus vannamei). To address this, we conducted BAC (bacterial artificial chromosome) end sequencing of L. vannamei. We selected and sequenced 7 812 BAC clones from the BAC library LvHE from the two ends of the inserts by Sanger sequencing. After trimming and quality filtering, 11 279 BAC end sequences (BESs) including 4 609 pairedends BESs were obtained. The total length of the BESs was 4 340 753 bp, representing 0.18% of the L. vannamei haploid genome. The lengths of the BESs ranged from 100 bp to 660 bp with an average length of 385 bp. Analysis of the BESs indicated that the L. vannamei genome is AT-rich and that the primary repeats patterns were simple sequence repeats (SSRs) and low complexity sequences. Dinucleotide and hexanucleotide repeats were the most common SSR types in the BESs. The most abundant transposable element was gypsy, which may contribute to the generation of the large genome size of L. vannamei. We successfully annotated 4 519 BESs by BLAST searching, including genes involved in immunity and sex determination. Our results provide an important resource for functional gene studies, map construction and integration, and complete genome assembly for this species.

  10. A comparative study on users’ satisfaction of library resource sharing under the circumstance of network in Nanjing

    Institute of Scientific and Technical Information of China (English)

    ZHU; Suoling; LIU; Lei

    2009-01-01

    From the perspective of information resource sharing,the authors investigated the ratio and degree of users’satisfaction with resource sharing among college libraries,public libraries and research libraries in Nanjing city.Based on the analysis and discussion about the findings,the authors summarized the library resource sharing system in satisfying users’information requirements,including its pros and cons.At last,further considerations have been provided on how to improve library resource sharing system in the region.

  11. Managing information resources in libraries collection management in theory and practice

    CERN Document Server

    Clayton, Peter

    2006-01-01

    The management of information resources in libraries is of greater importance in the digital world. This book encompasses different areas of collection management and cover topics, such as: collection management in the organizational context; collection development policies; selection principles and resources; budget management; and more.

  12. Factors Influencing Virtual Patron satisfaction with Online Library Resources and Services

    Directory of Open Access Journals (Sweden)

    Katherine Tyler

    2011-07-01

    Full Text Available College students are accessing virtual libraries whether they are on campus or learning from a distance. Academic institutions serving virtual patrons must remain focused on meeting the needs of those library users by continually examining their preferences, their searching behavior, and the information they seek. The purpose of this research was to determine if virtual patrons are satisfied with the resources and services being provided by a university’s online library. Following a web-based survey, demographic characteristics of students were analyzed to determine if any influenced students’ satisfaction. Using analysis of variance, correlation, and descriptive statistics, several demographic factors were found to influence student satisfaction with the library’s online resources: age, gender, achieved educational level, student status, and computer experience. One factor, computer experience, was found to influence student satisfaction with the library’s online services.Overall, students reported satisfaction with the university’s online library resources and services. Comments submitted to open-ended questions regarding areas for improvement to the online library provide library administrators with avenues for development to increase awareness of library services, focus improvement in navigation, and enhance student satisfaction.

  13. HIV/AIDS Information Resources from the National Library of Medicine-STOP

    Energy Technology Data Exchange (ETDEWEB)

    Templin-Branner, W. and N. Dancy

    2010-06-15

    The HIV/AIDS Information Resources from the National Library of Medicine training is designed specifically for the UNCFSP HBCU Screening, Testing, Outreach, and Prevention (STOP) HIV/AIDS Program project members to provide valuable health information resources from the National Library of Medicine and other reliable sources to increase awareness of the wealth of treatment information and educational materials that are available on the Internet and to improve prevention and treatment education for their clients. These resources will also meet the needs of community-based organizations

  14. Sequencing of 6.7 Mb of the melon genome using a BAC pooling strategy

    Directory of Open Access Journals (Sweden)

    Garcia-Mas Jordi

    2010-11-01

    Full Text Available Abstract Background Cucumis melo (melon belongs to the Cucurbitaceae family, whose economic importance among horticulture crops is second only to Solanaceae. Melon has a high intra-specific genetic variation, morphologic diversity and a small genome size (454 Mb, which make it suitable for a great variety of molecular and genetic studies. A number of genetic and genomic resources have already been developed, such as several genetic maps, BAC genomic libraries, a BAC-based physical map and EST collections. Sequence information would be invaluable to complete the picture of the melon genomic landscape, furthering our understanding of this species' evolution from its relatives and providing an important genetic tool. However, to this day there is little sequence data available, only a few melon genes and genomic regions are deposited in public databases. The development of massively parallel sequencing methods allows envisaging new strategies to obtain long fragments of genomic sequence at higher speed and lower cost than previous Sanger-based methods. Results In order to gain insight into the structure of a significant portion of the melon genome we set out to perform massive sequencing of pools of BAC clones. For this, a set of 57 BAC clones from a double haploid line was sequenced in two pools with the 454 system using both shotgun and paired-end approaches. The final assembly consists of an estimated 95% of the actual size of the melon BAC clones, with most likely complete sequences for 50 of the BACs, and a total sequence coverage of 39x. The accuracy of the assembly was assessed by comparing the previously available Sanger sequence of one of the BACs against its 454 sequence, and the polymorphisms found involved only 1.7 differences every 10,000 bp that were localized in 15 homopolymeric regions and two dinucleotide tandem repeats. Overall, the study provides approximately 6.7 Mb or 1.5% of the melon genome. The analysis of this new data has

  15. Publishing, Books and Library Resources: Brazil and Argentina.

    Science.gov (United States)

    National Book Committee, Inc., New York, NY.

    This survey synthesizes and analyzes the book resources and book requirements of Brazil, with particular reference to the educational scene, in terms of local production and distribution resources and capabilities. Information on the geography, political establishment, economy, and educational system of Brazil is presented in the introductory…

  16. DNA shuttling between plasmid vectors and a genome vector: systematic conversion and preservation of DNA libraries using the Bacillus subtilis genome (BGM) vector.

    Science.gov (United States)

    Kaneko, Shinya; Akioka, Manami; Tsuge, Kenji; Itaya, Mitsuhiro

    2005-06-24

    The combined use of the contemporary vector systems, the bacterial artificial chromosome (BAC) vector and the Bacillus subtilis genome (BGM) vector, makes possible the handling of giant-length DNA (above 100 kb). Our newly constructed BGM vector efficiently integrated DNA prepared in the BAC vector. A BAC library comprised of 18 independent clones prepared from mitochondrial DNA (mtDNA) of Arabidopsis thaliana was converted to a parallel BGM library using the new BGM vector. The effectiveness of the combined use of the vector systems was confirmed by the stable recovery of all 18 DNAs as BAC clones from the respective BGM clones. We show that DNA in BGM was stably preserved at room temperature after spore formation of the host B.subtilis. Rapid and stable shuttling between Escherichiacoli and the B. subtilis host, combined with spore-mediated DNA storage, may facilitate the long-term and low-cost preservation and the transportation of DNA resources. PMID:15913652

  17. The Open Library at AU (Athabasca University: Supporting Open Access and Open Educational Resources

    Directory of Open Access Journals (Sweden)

    Colin Elliott

    2015-04-01

    Full Text Available To address challenges that learners, course creators, librarians and academics involved with OER and MOOCs are facing when looking for scholarly materials, Athabasca University Library has initiated the development of the Open Library at AU. This open library is a full library website that provides easy access to open and free resources. Tools and information literacy tutorials are also included to enable learners, researchers, and others to find, evaluate, and use the information they need for their open learning course or research. Many of the challenges that those involved in open learning face are addressed by the open library and the potential impact it can have on open learning and knowledge sharing is tremendous. http://dx.doi.org/10.5944/openpraxis.7.2.196

  18. First Year Medical Students Use Library Resources Emphasized During Instruction Sessions

    Directory of Open Access Journals (Sweden)

    Laura Newton Miller

    2014-03-01

    Full Text Available Objective – To determine if library instruction has an effect on resources cited in student reports. Design – Citation analysis. Setting – The study took place in the medical school of a large American university. Subjects - One hundred eighteen of 120 first-year medical student reports were analyzed. Two reports did not include any works cited and were excluded from the study. Methods - Over the course of 3 years, 15 20-minute library instruction sessions were conducted. The sessions, based on five clinical cases presented each year were conducted approximately two weeks before each report due date. Eighty-five case-specific resources were demonstrated, with teaching plans being modified from year to year based on the frequency of citation of a particular resource cited the prior year. A LibGuide online course guide also directed students to specific resources shown in the class, with content updated every year based on citation trends from the previous year. Every citation referenced in a report was then categorized into a those that were discussed during an instruction session, b those found on a course guide, c those accessible through the library, d those available from course material (i.e., PowerPoint presentation, lecture notes, or e those which did not fall under any of the other categories. A citation could be included in multiple categories. Main Results – The 118 reports included 2983 citations. Over the 3 year period, an average of 77.51% of all citations were from library resources, 49.55% of the citations from a resource demonstrated in the class, and 21.68% from resources found in the course guide. Although citations from sources discussed in class did not increase significantly from year to year, the percent of citations from resources on the course guide significantly increased from 19.40% to 25.63%. Conclusion – Medical students cite library resources emphasized during instruction sessions.

  19. First Year Medical Students Use Library Resources Emphasized During Instruction Sessions

    OpenAIRE

    Laura Newton Miller

    2014-01-01

    Objective – To determine if library instruction has an effect on resources cited in student reports. Design – Citation analysis. Setting – The study took place in the medical school of a large American university. Subjects - One hundred eighteen of 120 first-year medical student reports were analyzed. Two reports did not include any works cited and were excluded from the study. Methods - Over the course of 3 years, 15 20-minute library instruction sessions were conducted. ...

  20. Use of Library Resources and Services by UG Students -A Study of NIT, Kurukshetra

    OpenAIRE

    Sushila Kumari; Prem Kumar; Anil Kumar

    2013-01-01

    The present study tries to know the use of library resources and services by UG Students of NIT,Kurukshetra. On the basis of the streams, 141 students were selected with the help of Stratified Sampling. The studyhighlights that the students visit the Library mainly for preparation of their course work and assignments (83.68%),examinations notes and tests (66.66%), preparation for workshops (39.71%) and tutorial presentations (16.31%).Maximum students considered that library collections (86.5%...

  1. Human resource management and career planning in a larger library

    OpenAIRE

    Jelka Gazvoda

    1997-01-01

    Human resource management is presented as a managerial function which is used to develop potential abilities of the employees to achieve organizational goals.Different perception of the employees is essential - people working in the organization are treated as capital and not as an expenditure. In human resource management the most important view of the employees is their potential growth and professional development, training for acquiring new responsibilities and encouragement for innovatio...

  2. Studium syntenie chromosomu Z obaleče jablečného (Cydia pomonella) (L.) metodou BAC-FISH.

    OpenAIRE

    Nguyen, Petr

    2009-01-01

    In this study, the codling moth (Cydia pomonella) partial sequences orthologous to genes that are Z-linked in other lepidopteran species were isolated and used as probes for screening of the codling moth BAC library. Selected BAC clones were labelled and mapped by fluorescence in situ hybridization (the so-called BAC-FISH) to codling moth chromosomes. Where BAC clones were unavailable, Z-linkage was confirmed by Southern hybridization with male and female genomic DNAs. The results revealed co...

  3. Extending the scope of Trove: addition of e-resources subscribed to by Australian Libraries.

    OpenAIRE

    Holley, Rose

    2011-01-01

    Trove is the national discovery service for Australia managed by the National Library of Australia. It was released in December 2009. It contains metadata for millions of freely accessible items, from more than 1,000 contributing institutions. The focus is on Australia and Australians. In 2011 it was developed further to include selected sets of e-resources subscribed to by Australian libraries. Trove v4.0 was released in May 2011 after 120 million subscription e-resources were successfully i...

  4. Library+

    Science.gov (United States)

    Merrill, Alex

    2011-01-01

    This article discusses possible future directions for academic libraries in the post Web/Library 2.0 world. These possible directions include areas such as data literacy, linked data sets, and opportunities for libraries in support of digital humanities. The author provides a brief sketch of the background information regarding the topics and…

  5. Linking Formal and Informal Science Education: A Successful Model using Libraries, Volunteers and NASA Resources

    Science.gov (United States)

    Race, M. S.; Lafayette Library; Learning Center Foundation (Lllcf)

    2011-12-01

    In these times of budget cuts, tight school schedules, and limited opportunities for student field trips and teacher professional development, it is especially difficult to expose elementary and middle school students to the latest STEM information-particularly in the space sciences. Using our library as a facilitator and catalyst, we built a volunteer-based, multi-faceted, curriculum-linked program for students and teachers in local middle schools (Grade 8) and showcased new astronomical and planetary science information using mainly NASA resources and volunteer effort. The project began with the idea of bringing free NASA photo exhibits (FETTU) to the Lafayette and Antioch Libraries for public display. Subsequently, the effort expanded by adding layers of activities that brought space and science information to teachers, students and the pubic at 5 libraries and schools in the 2 cities, one of which serves a diverse, underserved community. Overall, the effort (supported by a pilot grant from the Bechtel Foundation) included school and library based teacher workshops with resource materials; travelling space museum visits with hands-on activities (Chabot-to-Go); separate powerpoint presentations for students and adults at the library; and concurrent ancillary space-related themes for young children's programs at the library. This pilot project, based largely on the use of free government resources and online materials, demonstrated that volunteer-based, standards-linked STEM efforts can enhance curriculum at the middle school, with libraries serving a special role. Using this model, we subsequently also obtained a small NASA-Space Grant award to bring star parties and hand-on science activities to three libraries this Fall, linking with numerous Grade 5 teachers and students in two additional underserved areas of our county. It's not necessary to reinvent the wheel, you just collect the pieces and build on what you already have.

  6. Electronic Engineering Library In Sweden: A Quality Assessed Internet Resource from the Swedish Technological University Libraries

    OpenAIRE

    Lager, Gunnar; Nordlander, Marianne

    2012-01-01

    A status report of the plans and issues under discussion in creating this cooperative electronic national resource to support engineering education in Sweden. An opportunity to preview what is currently under construction for the EELS.

  7. Effective Techniques for the Promotion of Library Services and Resources

    Science.gov (United States)

    Yi, Zhixian

    2016-01-01

    Introduction: This study examines how Australian academic librarians perceive techniques for promoting services and resources, and the factors affecting the perceptions regarding effectiveness of techniques used. Method: Data were collected from an online survey that was sent to 400 academic librarians in thirty-seven Australian universities. The…

  8. BAC-Pool Sequencing and Assembly of 19 Mb of the Complex Sugarcane Genome

    Science.gov (United States)

    Okura, Vagner Katsumi; de Souza, Rafael S. C.; de Siqueira Tada, Susely F.; Arruda, Paulo

    2016-01-01

    Sequencing plant genomes are often challenging because of their complex architecture and high content of repetitive sequences. Sugarcane has one of the most complex genomes. It is highly polyploid, preserves intact homeologous chromosomes from its parental species and contains >55% repetitive sequences. Although bacterial artificial chromosome (BAC) libraries have emerged as an alternative for accessing the sugarcane genome, sequencing individual clones is laborious and expensive. Here, we present a strategy for sequencing and assembly reads produced from the DNA of pooled BAC clones. A set of 178 BAC clones, randomly sampled from the SP80-3280 sugarcane BAC library, was pooled and sequenced using the Illumina HiSeq2000 and PacBio platforms. A hybrid assembly strategy was used to generate 2,451 scaffolds comprising 19.2 MB of assembled genome sequence. Scaffolds of ≥20 Kb corresponded to 80% of the assembled sequences, and the full sequences of forty BACs were recovered in one or two contigs. Alignment of the BAC scaffolds with the chromosome sequences of sorghum showed a high degree of collinearity and gene order. The alignment of the BAC scaffolds to the 10 sorghum chromosomes suggests that the genome of the SP80-3280 sugarcane variety is ∼19% contracted in relation to the sorghum genome. In conclusion, our data show that sequencing pools composed of high numbers of BAC clones may help to construct a reference scaffold map of the sugarcane genome. PMID:27047520

  9. Integration of multimedia content and e-learning resources in a digital library

    Science.gov (United States)

    Pascual, Mireia; Ferran, Núria; Minguillón, Julià

    2006-01-01

    In this paper we describe a proposal for multimedia and e-learning content description based on standards interoperability within a digital library environment integrated in a virtual campus. In any virtual e-learning environment, a complex scenario which usually includes a digital library or, at least, a repository of learning resources, different levels of description are needed for all the elements: learning resources, multimedia content, activities, roles, etc. These elements can be described using library, e-learning and multimedia standards, depending on the specific needs of each particular scenario of use, but this might lead to an undesirable duplication of metadata, and to inefficient content queries and maintenance. Furthermore, there is a lack of semantic descriptions which makes all these contents merely become digital objects in the digital library, without exploiting all the possibilities in a e-learning virtual environment. Due to its flexibility and completeness, we propose to use the MPEG-7 standard for describing all the learning resources in the digital library, combined with the use of an ontology for a formal description of the learning process. The equivalences of Dublin Core, LOM and MPEG-7 standards are outlined, and the requirements of a proposal for a MPEG-7 based representation for all the contents in the digital library and the virtual classroom are described. The intellectual property policies for content sharing both within and among organizations are also addressed. With such proposal, it would be possible to build complex multimedia courses from a repository of learning objects using the digital library as the core repository.

  10. QR Codes as Finding Aides: Linking Electronic and Print Library Resources

    Science.gov (United States)

    Kane, Danielle; Schneidewind, Jeff

    2011-01-01

    As part of a focused, methodical, and evaluative approach to emerging technologies, QR codes are one of many new technologies being used by the UC Irvine Libraries. QR codes provide simple connections between print and virtual resources. In summer 2010, a small task force began to investigate how QR codes could be used to provide information and…

  11. Implementation of an Electronic Resource Assessment System in an Academic Library

    Science.gov (United States)

    Xu, Fei

    2010-01-01

    Purpose: The purpose of this paper is to provide a library with useful information about selection criteria for an electronic resource assessment system and practical assistance on how to implement efficiently such a system. Design/methodology/approach: The paper is based on literature review, desk research, and implementation experience.…

  12. Building Partnership: Gail M. Staines--Western NY Library Resources Council, Buffalo

    Science.gov (United States)

    Library Journal, 2004

    2004-01-01

    This article details the work of Gail Staines, who is probably the only Mover & Shaker who lists the U.S.A. Equestrians Association among her professional memberships. But it is not that far a leap from what she does in her work as director of the Western New York Library Resources Council (WNYLRC), where she is responsible for making 98 libraries…

  13. School Library Supplement. Putting Your Library Automation System to Work [and] Using Videodiscs in Research Projects [and] Library Security: What to Look For [and] Expanding Library/Media Services with Media Retrieval [and] How to Network CD-ROMs (and) The School Library Resource Directory.

    Science.gov (United States)

    Karpisek, Marian; And Others

    1995-01-01

    Presents five articles and a company resource directory to help librarians successfully incorporate technology into school libraries. Discusses actual situations, examines student needs, and gives advice to help librarians with library automation systems, videodiscs, library security systems, media retrieval, networking CD-ROMs, and locating…

  14. Caribbean Equal Access Program: HIV/AIDS Information Resources from the National Library of Medicine

    Energy Technology Data Exchange (ETDEWEB)

    Nancy Dancy, NLM, and Wilma Templin-Branner, ORISE

    2009-01-01

    As the treatment and management of HIV/AIDS continues to evolve with new scientific breakthroughs, treatment discoveries, and management challenges, it is difficult for people living with HIV/AIDS and those who care for them to keep up with the latest information on HIV/AIDS prevention, treatment, and research. The National Library of Medicine, of the National Institutes of Health, has a wealth of health information resources freely available on the Internet to address these needs.

  15. Open and Editable: Exploring Library Engagement in Open Educational Resource Adoption, Adaptation and Authoring

    OpenAIRE

    Walz, Anita R.

    2015-01-01

    Open Educational Resources (OER) have saved students millions of dollars in textbook costs and greatly expanded access to a wide variety of educational materials for countless numbers of students and life-long learners. OER have also saved teachers time and effort by allowing them to reuse, modify, and build on materials developed by other teachers. After a brief discussion of OER and foundations of open licensing, this article presents a number of opportunities for libraries, particularly...

  16. Library and Information Resources and Users of Digital Resources in the Humanities

    Science.gov (United States)

    Warwick, Claire; Terras, Melissa; Galina, Isabel; Huntington, Paul; Pappa, Nikoleta

    2008-01-01

    Purpose: The purpose of this article is to discuss the results of the Log Analysis of Internet Resources in the Arts and Humanities (LAIRAH) study. It aims to concentrate upon the use and importance of information resources, physical research centres and digital finding aids in scholarly research. Design/methodology/approach: Results are presented…

  17. Využití BAC klonů při studiu pohlavního chromosomu W obaleče jablečného Cydia pomonella (Lepidoptera: Tortricidae)

    OpenAIRE

    Dalíková, Martina

    2009-01-01

    In the present study, the W sex-chromosome of the codling moth was studied by means of fluorescence in situ hybridization (FISH) with probes prepared from bacterial artificial chromosome (BAC), which were isolated from the codling moth BAC library. The BAC library was screened for clones derived from both the W and Z sex chromosomes using three sets of molecular markers of codling moth sex chromosomes. A total of 54 BAC clones have been obtained. In this work, only 3 W-derived BAC clones and ...

  18. Library and information resources and users of digital resources in the humanities.

    OpenAIRE

    C. Warwick; Terras, M.; Galina, I.; Huntington, P.; Pappa, N.

    2008-01-01

    Purpose – The purpose of this article is to discuss the results of the Log Analysis of Internet Resources in the Arts and Humanities (LAIRAH) study. It aims to concentrate upon the use and importance of information resources, physical research centres and digital finding aids in scholarly research. Design/methodology/approach – Results are presented of web server log analysis of portals for humanities scholars: the arts and humanities data service (AHDS) website and Humbul Humanities Hub....

  19. Out on the Web: The Relationship between Campus Climate and GLBT-Related Web-based Resources in Academic Libraries

    Science.gov (United States)

    Ciszek, Matthew P.

    2011-01-01

    This article explores the relationship between the perceived campus environment for gay, lesbian, bisexual, and transgender (GLBT) students at colleges and universities and how academic libraries have deployed GLBT-related resources on the Web. Recommendations are made for increasing GLBT-related materials and information in academic libraries.…

  20. Planning for a Multi-Campus Merger: Implications for Library Services and Resources at Penn State University's Capital College.

    Science.gov (United States)

    Stimatz, Lisa R.; White, Gary W.; Crawford, Gregory A.

    1997-01-01

    Describes the strategic planning process for the merger between the libraries of Penn State University's Harrisburg and Schuykill campuses. Examines the merger's potential impact on library services and resources. Discusses possible solutions to meeting the information needs of the merged institutions. (Author/AEF)

  1. Effects of Blood-Alcohol Concentration (BAC) Feedback on BAC Estimates Over Time

    Science.gov (United States)

    Bullers, Susan; Ennis, Melissa

    2006-01-01

    This study examines the effects of self-tested blood alcohol concentration (BAC) feedback, from personal hand-held breathalyzers, on the accuracy of BAC estimation. Using an e-mail prompted web-based questionnaire, 19 participants were asked to report both BAC estimates and subsequently measured BAC levels over the course of 27 days. Results from…

  2. A Study of Assessment of Library E-resource Metasearch System

    Directory of Open Access Journals (Sweden)

    Fan-Chuan Tseng

    2008-12-01

    Full Text Available As the emergent development of computer and network technology, digital library and information service has become an important approach for users to increase their academic as well as job performance due to the authorized, accurate, and reliable information. In the realm of information management, the exploration of factors influencing information system utilization and the evaluation of benefits are two critical issues. However, related studies focus more on the system functions and usually ignore the effects of individual cognition and social influence. In this study, we extend the IS success model with two additional factors-self-efficacy and subjective norm, trying to explore the potential influences on digital library usage, user satisfaction and performance.This research takes the Chang Jung Christian University library E-resources Metasearch system as the case study. A questionnaire was developed and finally got 160 useful student samples to test and verify the hypotheses. The research results confirmed that self-efficacy and subjective norm can significantly affect the usage of E-resource Metasearch System. Subsequently, the users feel satisfied with the system, and increase their academic performance. Finally, we provide discussion, implications and suggestions in two aspects – cooperating more with educational programs to increase users’ information literacy, and improving the customer-oriented system interface, functions, and services to enhance its quality. [Article content in Chinese

  3. E-resources measurement in libraries Misurazione delle risorse elettroniche in biblioteca

    Directory of Open Access Journals (Sweden)

    Ilaria Fava

    2011-12-01

    Full Text Available The article aims at presenting a review of standards and current international initiatives on academic libraries evaluation and assessment, with the analysis of indicators related to electronic resources measurement. On electronic resources there are given some definition useful to understand both indicators and measures. The review of the international literature show that the emergent tendency is to collect and evaluate quantitative data related to the "health" of academic libraries together with qualitative data on services offered to library users.
    L'articolo si propone di illustrare gli standard e le iniziative internazionali sulla valutazione delle biblioteche accademiche, con l’analisi degli indicatori che riguardano in modo più approfondito le risorse elettroniche. Sulle risorse elettroniche, inoltre, vengono fornite una serie di definizioni utili a comprendere le misure richieste. L’analisi della letteratura internazionale fa emergere una tendenza alla raccolta e alla valutazione non solo di dati puramente quantitativi sulla “salute” delle biblioteche accademiche, ma anche di dati sulla qualità dell’offerta di servizi agli utenti.

  4. Federal Agency and Federal Library Reports. Library of Congress; Center for the Book; Federal Library and Information Center Committee; National Commission on Libraries and Information Science; National Agricultural Library; National Library of Medicine;United States Government Printing Office; National Technical Information Service; National Archives and Records Administration; National Center for Education Statistics Library Statistics Program; National Library of Education; Educational Resources Information Center.

    Science.gov (United States)

    Fischer, Audrey; Cole, John Y.; Tarr, Susan M.; Vlach, Rosalie B.; Carey, Len; Mehnert, Robert; Sherman, Andrew M.; Davis, Linda; Vecchiarelli, Marion H.; Chute, Adrienne; Dunn, Christina

    2002-01-01

    Includes reports from Library of Congress, Center for the Book, Federal Library and Information Center Committee, National Commission on Libraries and Information Science, National Agricultural Library, National Library of Medicine, Government Printing Office, National Technical Information Service, National Archives and Records Administration,…

  5. A Comparative BAC Map for the Gilthead Sea Bream (Sparus aurata L.

    Directory of Open Access Journals (Sweden)

    Heiner Kuhl

    2011-01-01

    Full Text Available This study presents the first comparative BAC map of the gilthead sea bream (Sparus aurata, a highly valuated marine aquaculture fish species in the Mediterranean. High-throughput end sequencing of a BAC library yielded 92,468 reads (60.6 Mbp. Comparative mapping was achieved by anchoring BAC end sequences to the three-spined stickleback (Gasterosteus aculeatus genome. BACs that were consistently ordered along the stickleback chromosomes accounted for 14,265 clones. A fraction of 5,249 BACs constituted a minimal tiling path that covers 73.5% of the stickleback chromosomes and 70.2% of the genes that have been annotated. The N50 size of 1,485 “BACtigs” consisting of redundant BACs is 337,253 bp. The largest BACtig covers 2.15 Mbp in the stickleback genome. According to the insert size distribution of mapped BACs the sea bream genome is 1.71-fold larger than the stickleback genome. These results represent a valuable tool to researchers in the field and may support future projects to elucidate the whole sea bream genome.

  6. 棉花细菌人工染色体文库构建方法探讨%Studies on Construction Method of Cotton Bacterial Artificial Chromosome Library

    Institute of Scientific and Technical Information of China (English)

    高海燕; 王省芬; 刘方; 彭仁海; 张艳; 马峙英; 王坤波

    2013-01-01

    细菌人工染色体(Bacterial artificial chromosome,BAC)文库是开展基因组测序、基因图位克隆、分子标记、物理作图等研究的重要基因组资源.本文在构建了二倍体野生棉阿非利加棉(Gossypium herbaceum var.africanum)BAC文库的基础上,就棉花细菌人工染色体基因组文库构建过程中高分子量基因组DNA的提取、部分酶切片段选择、DNA的回收、连接转化以及BAC文库的保存等过程中一些细节和注意事项进行了比较详细的分析比较,希望能为棉花BAC文库的构建提供一些可供借鉴的经验.%Bacterial artificial chromosome (BAC) library is an important genome resources to such research as genome sequencing, map-based cloning, molecular markers, and physical mapping. On the base of the construction of BAC library for Gossypi-um herbaceum var. africanum, this paper presents an exhaustive analysis on details and notices of the BAC library construction process. It includes extraction of high molecular weight (HMW) nuclear DNA, determination of the optimized enzyme for partial digestion of HMW DNA, two rounds of size fractionation, recovery of large fragments DNA, ligation and transformation of large fragments of DNA and storage of BAC library. Thus being able to supply an experience for constructing high efficiency cotton BAC library.

  7. Providing Curriculum Support in the School Library Media Center: Resource Alignment, or How To Eat an Elephant.

    Science.gov (United States)

    Lowe, Karen

    2003-01-01

    Discusses the process of weeding, updating, and building a school library media collection that supports the state curriculum. Explains resource alignment, a process for using the shelf list as a tool to analyze and align media center resources to state curricula, and describes a five-year plan and its usefulness for additional funding. (LRW)

  8. Preference and Use of Electronic Information and Resources by Blind/Visually Impaired in NCR Libraries in India

    OpenAIRE

    Shailendra Kumar; Gareema Sanaman

    2013-01-01

    This paper aims to determine the preference and use of electronic information and resources by blind/visually impaired users in the leading National Capital Region (NCR) libraries of India. Survey methodology has been used as the basic research tool for data collection with the help of questionnaires. The 125 in total users surveyed in all the five libraries were selected randomly on the basis of willingness of the users with experience of working in digital environments to participate in the...

  9. Simplifying resource discovery and access in academic libraries : implementing and evaluating Summon at Huddersfield and Northumbria Universities

    OpenAIRE

    Thoburn, June; Coates, Annette; Stone, Graham

    2010-01-01

    Facilitating information discovery and maximising value for money from library materials is a key driver for academic libraries, which spend substantial sums of money on journal, database and book purchasing. Users are confused by the complexity of our collections and the multiple platforms to access them and are reluctant to spend time learning about individual resources and how to use them - comparing this unfavourably to popular and intuitive search engines like Google. As a...

  10. Astronomy for Everyone! -- The Library of Arcetri Astrophysical Observatory and the Public: An Example of Integration of Information Resources

    Science.gov (United States)

    Brunetti, F.; Gasperini, A.; Albanese, L.

    2007-10-01

    This talk illustrates how a research library can work in the public communication of science. It describes some experiences at Arcetri Astrophysical Observatory library in the dissemination of astronomical information for the general public. Particular attention is devoted to (1) the integration of information resources to support public outreach to children; and (2) the use of the historical archives for the dissemination of the history of astronomy.

  11. Data Management inside the Library: Assessing Electronic Resources Data Using the Data Asset Framework Methodology

    Science.gov (United States)

    Ogier, Andi; Hall, Monena; Bailey, Annette; Stovall, Connie

    2014-01-01

    Rapidly growing within academic libraries, library data services have often been focused on assessing research trends and building partnerships outside the library. There are distinct benefits, however, to using data audit methodologies created for these external assessments of researcher practices inside the library as well. In this article, we…

  12. "The Open Library at AU" (Athabasca University): Supporting Open Access and Open Educational Resources

    Science.gov (United States)

    Elliott, Colin; Fabbro, Elaine

    2015-01-01

    To address challenges that learners, course creators, librarians and academics involved with OER and MOOCs are facing when looking for scholarly materials, Athabasca University Library has initiated the development of "the Open Library at AU." This open library is a full library website that provides easy access to open and free…

  13. Toward a Molecular Cytogenetic Map for Cultivated Sunflower (Helianthus annuus L.) by Landed BAC/BIBAC Clones

    OpenAIRE

    Feng, Jiuhuan; Liu, Zhao; Cai, Xiwen; Jan, Chao-Chien

    2013-01-01

    Conventional karyotypes and various genetic linkage maps have been established in sunflower (Helianthus annuus L., 2n = 34). However, the relationship between linkage groups and individual chromosomes of sunflower remains unknown and has considerable relevance for the sunflower research community. Recently, a set of linkage group-specific bacterial /binary bacterial artificial chromosome (BAC/BIBAC) clones was identified from two complementary BAC and BIBAC libraries constructed for cultivate...

  14. A bacterial artificial chromosome library for the Australian saltwater crocodile (Crocodylus porosus) and its utilization in gene isolation and genome characterization

    Science.gov (United States)

    2009-01-01

    Background Crocodilians (Order Crocodylia) are an ancient vertebrate group of tremendous ecological, social, and evolutionary importance. They are the only extant reptilian members of Archosauria, a monophyletic group that also includes birds, dinosaurs, and pterosaurs. Consequently, crocodilian genomes represent a gateway through which the molecular evolution of avian lineages can be explored. To facilitate comparative genomics within Crocodylia and between crocodilians and other archosaurs, we have constructed a bacterial artificial chromosome (BAC) library for the Australian saltwater crocodile, Crocodylus porosus. This is the first BAC library for a crocodile and only the second BAC resource for a crocodilian. Results The C. porosus BAC library consists of 101,760 individually archived clones stored in 384-well microtiter plates. NotI digestion of random clones indicates an average insert size of 102 kb. Based on a genome size estimate of 2778 Mb, the library affords 3.7 fold (3.7×) coverage of the C. porosus genome. To investigate the utility of the library in studying sequence distribution, probes derived from CR1a and CR1b, two crocodilian CR1-like retrotransposon subfamilies, were hybridized to C. porosus macroarrays. The results indicate that there are a minimum of 20,000 CR1a/b elements in C. porosus and that their distribution throughout the genome is decidedly non-random. To demonstrate the utility of the library in gene isolation, we probed the C. porosus macroarrays with an overgo designed from a C-mos (oocyte maturation factor) partial cDNA. A BAC containing C-mos was identified and the C-mos locus was sequenced. Nucleotide and amino acid sequence alignment of the C. porosus C-mos coding sequence with avian and reptilian C-mos orthologs reveals greater sequence similarity between C. porosus and birds (specifically chicken and zebra finch) than between C. porosus and squamates (green anole). Conclusion We have demonstrated the utility of the

  15. Charting a Course through CORAL: Texas A&M University Libraries' Experience Implementing an Open-Source Electronic Resources Management System

    Science.gov (United States)

    Hartnett, Eric; Beh, Eugenia; Resnick, Taryn; Ugaz, Ana; Tabacaru, Simona

    2013-01-01

    In 2010, after two previous unsuccessful attempts at electronic resources management system (ERMS) implementation, Texas A&M University (TAMU) Libraries set out once again to find an ERMS that would fit its needs. After surveying the field, TAMU Libraries selected the University of Notre Dame Hesburgh Libraries-developed, open-source ERMS, CORAL…

  16. Comparative BAC-based mapping in the white-throated sparrow, a novel behavioral genomics model, using interspecies overgo hybridization

    Directory of Open Access Journals (Sweden)

    Gonser Rusty A

    2011-06-01

    Full Text Available Abstract Background The genomics era has produced an arsenal of resources from sequenced organisms allowing researchers to target species that do not have comparable mapping and sequence information. These new "non-model" organisms offer unique opportunities to examine environmental effects on genomic patterns and processes. Here we use comparative mapping as a first step in characterizing the genome organization of a novel animal model, the white-throated sparrow (Zonotrichia albicollis, which occurs as white or tan morphs that exhibit alternative behaviors and physiology. Morph is determined by the presence or absence of a complex chromosomal rearrangement. This species is an ideal model for behavioral genomics because the association between genotype and phenotype is absolute, making it possible to identify the genomic bases of phenotypic variation. Findings We initiated a genomic study in this species by characterizing the white-throated sparrow BAC library via filter hybridization with overgo probes designed for the chicken, turkey, and zebra finch. Cross-species hybridization resulted in 640 positive sparrow BACs assigned to 77 chicken loci across almost all macro-and microchromosomes, with a focus on the chromosomes associated with morph. Out of 216 overgos, 36% of the probes hybridized successfully, with an average number of 3.0 positive sparrow BACs per overgo. Conclusions These data will be utilized for determining chromosomal architecture and for fine-scale mapping of candidate genes associated with phenotypic differences. Our research confirms the utility of interspecies hybridization for developing comparative maps in other non-model organisms.

  17. DEVELOPMENTAL PROJECTS OF UNIVERSITY LIBRARIES PROVIDING ACCESS TO SCIENTIFIC E-RESOURCES FINANCED FROM NATIONAL AND EU FUNDS

    OpenAIRE

    Willecki, Jacek

    2016-01-01

    Scientific libraries at universities are interested in transforming traditional scientific resources in the print form into their digital equivalents and also in providing, when possible, a free access to these resources via the Internet. The development of digital versions of scientific work and research and providing access to them can be financed from sources other than the university budget. An overview of main external sources of funding and an analysis of project documentation indicate ...

  18. Herpesvirus BACs: Past, Present, and Future

    Directory of Open Access Journals (Sweden)

    Charles Warden

    2011-01-01

    Full Text Available The herpesviridae are a large family of DNA viruses with large and complicated genomes. Genetic manipulation and the generation of recombinant viruses have been extremely difficult. However, herpesvirus bacterial artificial chromosomes (BACs that were developed approximately 10 years ago have become useful and powerful genetic tools for generating recombinant viruses to study the biology and pathogenesis of herpesviruses. For example, BAC-directed deletion mutants are commonly used to determine the function and essentiality of viral genes. In this paper, we discuss the creation of herpesvirus BACs, functional analyses of herpesvirus mutants, and future applications for studies of herpesviruses. We describe commonly used methods to create and mutate herpesvirus BACs (such as site-directed mutagenesis and transposon mutagenesis. We also evaluate the potential future uses of viral BACs, including vaccine development and gene therapy.

  19. Extending Geant4 Parallelism with External Libraries (MPI, TBB) and Its Use on HPC Resources

    CERN Document Server

    Dotti, Andrea; Barrand, Guy; Hrivnacova, Ivana; Murakami, Koichi

    2016-01-01

    With Geant4 Version 10.0, released in December 2013, one of the most widely used Monte-Carlo codes has been ported to take full advantage of multi- and many-core CPUs thanks to the introduction of event-level parallelism via multithreading. In this paper we review recent developments to allow for a better integration of parallel Geant4 jobs with external libraries. We have chosen to develop examples using the popular Intel Threading Building Block (for short TBB) as an alternative parallelization approach to the native Geant4 POSIX. To simplify the scaling of a Geant4 application across nodes on a cluster we are improving the support of MPI in Geant4. In particular it is now possible to run an hybrid MPI/MT application that uses MPI to scale across nodes and MT to scale across cores. %The recent developments allow users to easily implement parallel application resources that scale on a very large number of nodes and cores typical of HPC resources.

  20. Planning the new medical school library in relation to local and regional information resources.

    Science.gov (United States)

    Bishop, D

    1971-04-01

    The first relationship of a new medical school library with local and regional medical libraries is one of dependence. This must change, as the new library grows, to a continuing state of interdependence. To accomplish this, the new library must thoughtfully design its collections and services, and must prepare for an extramural as well as an intramural clientele. Relationships with Regional Medical Library services and with Regional Medical Programs must be carefully planned. So too must be the adoption of EDP and related procedures where future compatability is important. Any new medical school library must have strength, but must never forget the need for true cooperation to give it viability. PMID:5582095

  1. The Role of Free Internet Resources for Library Technical Services and Reference in a Hungarian LIS Continuing Education Course

    Science.gov (United States)

    Koltay, Tibor

    2006-01-01

    After providing background to Continuing Professional Education (CPE) of librarians in Hungary, this article outlines the content of a course that seeks to make Hungarian librarians familiar with free Internet resources that are usable for library technical services and reference. One of the most important topics covered in the course is the…

  2. The Availability and Utilization of School Library Resources in Some Selected Secondary Schools (High School) in Rivers State

    Science.gov (United States)

    Owate, C. N.; Iroha, Okpa

    2013-01-01

    This study investigates the availability and utilization of school library resources by Secondary School (High School) Students. Eight Selected Secondary Schools in Rivers State, Nigeria were chosen based on their performance in external examinations and geographic locations. In carrying out the research, questionnaires were administered to both…

  3. Impact of Electronic Resources and Usage in Academic Libraries in Ghana: Evidence from Koforidua Polytechnic & All Nations University College, Ghana

    Science.gov (United States)

    Akussah, Maxwell; Asante, Edward; Adu-Sarkodee, Rosemary

    2015-01-01

    The study investigates the relationship between impact of electronic resources and its usage in academic libraries in Ghana: evidence from Koforidua Polytechnic & All Nations University College, Ghana. The study was a quantitative approach using questionnaire to gather data and information. A valid response rate of 58.5% was assumed. SPSS…

  4. How Can Libraries Determine the Value in Collecting, Managing, Preserving, and/or Cataloging E-Resources?

    Science.gov (United States)

    King, Douglas

    2009-01-01

    The author recently asked his colleagues, "How can libraries determine the value in collecting, managing, preserving, and/or cataloging e-resources?" hoping to challenge readers to come up with some thoughtful opinions and useful tips. Not surprisingly, he received a handful of substantially different but uniformly provocative and creative…

  5. Central New York Library Resources Council CLRC Regional Digitization Plan. Final Report for the Preparing Central New York History for the Future LSTA Project.

    Science.gov (United States)

    Sywetz, Betsy

    The primary goal for digitization projects sponsored by the Central New York Library Resources Council (CLRC) is enhanced access for the people of the region to digital resources created from collections in Central New York's libraries, archives and museums. The CLRC Digitization Plan provides a framework for the support of digitization activities…

  6. GeoDeepDive: Towards a Machine Reading-Ready Digital Library and Information Integration Resource

    Science.gov (United States)

    Husson, J. M.; Peters, S. E.; Livny, M.; Ross, I.

    2015-12-01

    Recent developments in machine reading and learning approaches to text and data mining hold considerable promise for accelerating the pace and quality of literature-based data synthesis, but these advances have outpaced even basic levels of access to the published literature. For many geoscience domains, particularly those based on physical samples and field-based descriptions, this limitation is significant. Here we describe a general infrastructure to support published literature-based machine reading and learning approaches to information integration and knowledge base creation. This infrastructure supports rate-controlled automated fetching of original documents, along with full bibliographic citation metadata, from remote servers, the secure storage of original documents, and the utilization of considerable high-throughput computing resources for the pre-processing of these documents by optical character recognition, natural language parsing, and other document annotation and parsing software tools. New tools and versions of existing tools can be automatically deployed against original documents when they are made available. The products of these tools (text/XML files) are managed by MongoDB and are available for use in data extraction applications. Basic search and discovery functionality is provided by ElasticSearch, which is used to identify documents of potential relevance to a given data extraction task. Relevant files derived from the original documents are then combined into basic starting points for application building; these starting points are kept up-to-date as new relevant documents are incorporated into the digital library. Currently, our digital library stores contains more than 360K documents supplied by Elsevier and the USGS and we are actively seeking additional content providers. By focusing on building a dependable infrastructure to support the retrieval, storage, and pre-processing of published content, we are establishing a foundation for

  7. Constructing library networks with Chinese characteristics: bringing about society-wide sharing of information resources

    OpenAIRE

    Salman Nazari

    2006-01-01

    As one of the most important components of a nations basic facilities, the networking of the library plays a vital role in terms of promoting the sharing of access to information. Although the traditional methods of literature indexing and accessing will continue to exist and develop, library networking will become the new model and the direction for the development of the library. Through the discussion of the current situation of information sharing in the Chinese library, the author analyz...

  8. Selected indicators of the range and use of printed resources in Polish academic libraries

    OpenAIRE

    Górny, Mirosław; Jazdon, Artur; Nowak, Piotr

    1999-01-01

    Reports the results of a survey rating the amount and degree of the utilisation of printed materials in Polish academic libraries. The results which are presented by type (for example chemical, biological, technical libraries etc.) as well as by function (research libraries and libraries of educational institutions), concern the number of materials, including both books and periodicals. The authors include the amount of material in English as well as in other foreign l...

  9. Electronic Resource Expenditure and the Decline in Reference Transaction Statistics in Academic Libraries

    Science.gov (United States)

    Dubnjakovic, Ana

    2012-01-01

    The current study investigates factors influencing increase in reference transactions in a typical week in academic libraries across the United States of America. Employing multiple regression analysis and general linear modeling, variables of interest from the "Academic Library Survey (ALS) 2006" survey (sample size 3960 academic libraries) were…

  10. The Personnel, Material and Financial Resources of the Library Systems of 311 Industrial Corporations.

    Science.gov (United States)

    Jackson, Eugene B.; Jackson, Ruth L.

    An indepth study of the FORTUNE 500 Corporations' libraries has revealed consistent characteristics across 27 industrial classifications, some of which parallel the divisions of professional associations, such as the Special Libraries Association. Partial profiles of Pharmaceutical, Aircraft, and Food Libraries are given as examples, with the…

  11. Preference and Use of Electronic Information and Resources by Blind/Visually Impaired in NCR Libraries in India

    Directory of Open Access Journals (Sweden)

    Shailendra Kumar

    2013-06-01

    Full Text Available This paper aims to determine the preference and use of electronic information and resources by blind/visually impaired users in the leading National Capital Region (NCR libraries of India. Survey methodology has been used as the basic research tool for data collection with the help of questionnaires. The 125 in total users surveyed in all the five libraries were selected randomly on the basis of willingness of the users with experience of working in digital environments to participate in the survey. The survey results were tabulated and analyzed with descriptive statistics methods using Excel software and 'Stata version 11'. The findings reveal that ICT have a positive impact in the lives of people with disabilities as it helps them to work independently and increases the level of confidence among them. The Internet is the most preferred medium of access to information among the majority of blind/visually impaired users. The 'Complexity of content available on the net' is found as the major challenge faced during Internet use by blind users of NCR libraries. 'Audio books on CDs/DVDs and DAISY books' are the most preferred electronic resources among the majority of blind/visually impaired users. This study will help the library professionals and organizations/institutions serving people with disabilities to develop effective library services for blind/visually impaired users in the digital environment on the basis of findings on information usage behavior in the study.

  12. Intra- and interchromosomal rearrangements between cowpea [Vigna unguiculata (L.) Walp.] and common bean (Phaseolus vulgaris L.) revealed by BAC-FISH.

    Science.gov (United States)

    Vasconcelos, Emanuelle Varão; de Andrade Fonsêca, Artur Fellipe; Pedrosa-Harand, Andrea; de Andrade Bortoleti, Kyria Cilene; Benko-Iseppon, Ana Maria; da Costa, Antônio Félix; Brasileiro-Vidal, Ana Christina

    2015-06-01

    Cowpea (Vigna unguiculata) is an annual legume grown in tropical and subtropical regions, which is economically relevant due to high protein content in dried beans, green pods, and leaves. In this work, a comparative cytogenetic study between V. unguiculata and Phaseolus vulgaris (common bean) was conducted using BAC-FISH. Sequences previously mapped in P. vulgaris chromosomes (Pv) were used as probes in V. unguiculata chromosomes (Vu), contributing to the analysis of macrosynteny between both legumes. Thirty-seven clones from P. vulgaris 'BAT93' BAC library, corresponding to its 11 linkage groups, were hybridized in situ. Several chromosomal rearrangements were identified, such as translocations (between BACs from Pv1 and Pv8; Pv2 and Pv3; as well as Pv2 and Pv11), duplications (BAC from Pv3), as well as paracentric and pericentric inversions (BACs from Pv3, and Pv4, respectively). Two BACs (from Pv2 and Pv7), which hybridized at terminal regions in almost all P. vulgaris chromosomes, showed single-copy signal in Vu. Additionally, 17 BACs showed no signal in V. unguiculata chromosomes. The present results demonstrate the feasibility of using BAC libraries in comparative chromosomal mapping and karyotype evolution studies between Phaseolus and Vigna species, and revealed several macrosynteny and collinearity breaks among both legumes. PMID:25634499

  13. Keeping Libraries Relevant in the Semantic Web with RDA: Resource Description and Access

    Directory of Open Access Journals (Sweden)

    Barbara Tillett

    2011-12-01

    Full Text Available Catalogare non vuol dire semplicemente costruire un catalogo. Vuol dire far sì che gli utenti accedano tempestivamente alle informazioni pertinenti alle loro esigenze. Il lavoro di identificazione delle risorse raccolte da biblioteche, archivi, musei, dà luogo a ricchi metadati che possono essere riutilizzati per molti scopi ("le attività dell’utente". Ciò comporta la descrizione delle risorse e il mostrare le loro relazioni con persone, famiglie, enti e altre risorse, consentendo così agli utenti di navigare attraverso surrogati delle risorse per ottenere più rapidamente le informazioni di cui hanno bisogno. I metadati costruiti lungo tutto il ciclo di vita di una risorsa sono particolarmente preziosi per molti tipi di utenti: dai creatori delle risorse, agli editori, alle agenzie, ai librai, agli aggregatori di risorse, ai fornitori di sistemi, alle biblioteche, ad altre istituzioni culturali ed agli utenti finali. Il nuovo codice internazionale di catalogazione, RDA: Resource Description e Access, è progettato per soddisfare le attività di base degli utenti producendo metadati ben formati e interconnessi per l'ambiente digitale, dando la possibilità alle biblioteche di rimanere rilevanti nel web semantico. Acknowledge Il testo inglese del saggio è pubblicato in "Serials", November 2011, 24, 3, con il titolo Keeping Libraries Relevant in the Semantic Web with RDA: Resource Description and Access, DOI: http://dx.doi.org/10.1629/24266. Traduzione italiana di Maria Chiara Iorio e Tiziana Possemato, che ringraziano Carlo Bianchini e Mauro Guerrini per la rilettura della traduzione.

  14. Defining an open access resource strategy for research libraries:Part Ⅱ——Challenges and responses

    Institute of Scientific and Technical Information of China (English)

    Xiaolin; ZHANG; Yan; ZENG; Lin; LI; Xiwen; LIU

    2012-01-01

    Purpose:This paper aims to provide a systematic analysis of challenges and responses of the imminent open access-based environment so as to enable sound development of open access(OA)resource strategies.Design/methodology/approach:A systematic and evidence-based analysis is used to discover,identify,and contextualize the factors that relate or interact.A challenge metric for the overall context and additional frameworks or alternative lists is used for the detailed analysis.Findings:Research libraries are facing a reality conflict between the traditional library solution and the OA solution.New resource strategies are needed but have to be based on an open knowledge infrastructure and a systematic analysis of factors and alternatives.Support for OA publishing is becoming a major task and complicated capabilities involved call for libraries to play a central role.Hybrid collection development should be in place to take full advantages of the open world while ensuring reliable and trusted retrieval,reuse,and preservation.Research limitations:The research covers main developments in US and European countries mostly in scientific fields and for scholarly papers.Practical implications:There are already evidences for sound OA resource policies.With the imminent dominance of OA resources,research libraries should and can set up their OA resource strategies.Originality/value:The paper suggests a factor metric as an analysis framework,presents rationale lists and policies to support OA publishing,and proposes a hybrid collection strategy to plan for collection development,retrieval,and preservation.

  15. A BAC-based physical map of Zhikong scallop (Chlamys farreri Jones et Preston.

    Directory of Open Access Journals (Sweden)

    Xiaojun Zhang

    Full Text Available Zhikong scallop (Chlamys farreri is one of the most economically important aquaculture species in China. Physical maps are crucial tools for genome sequencing, gene mapping and cloning, genetic improvement and selective breeding. In this study, we have developed a genome-wide, BAC-based physical map for the species. A total of 81,408 clones from two BAC libraries of the scallop were fingerprinted using an ABI 3130xl Genetic Analyzer and a fingerprinting kit developed in our laboratory. After data processing, 63,641 (∼5.8× genome coverage fingerprints were validated and used in the physical map assembly. A total of 3,696 contigs were assembled for the physical map. Each contig contained an average of 10.0 clones, with an average physical size of 490 kb. The combined total physical size of all contigs was 1.81 Gb, equivalent to approximately 1.5 fold of the scallop haploid genome. A total of 10,587 BAC end sequences (BESs and 167 markers were integrated into the physical map. We evaluated the physical map by overgo hybridization, BAC-FISH (fluorescence in situ hybridization, contig BAC pool screening and source BAC library screening. The results have provided evidence of the high reliability of the contig physical map. This is the first physical map in mollusc; therefore, it provides an important platform for advanced research of genomics and genetics, and mapping of genes and QTL of economical importance, thus facilitating the genetic improvement and selective breeding of the scallop and other marine molluscs.

  16. Innovation on hospital library human resources management%医院图书馆人力资源管理创新

    Institute of Scientific and Technical Information of China (English)

    王玉双

    2014-01-01

    Based on the problems which can described as backward management of human resources, librarian quality, brain drain and irrational human resources structure of human resources management in hospital library, new concept of hospital library human resources management is presented. Suggestions such as implementing scientific human resources management and training, post appointment and performance system and making human resources management policy are also discussed.%结合制约医院图书馆发展中人力资源管理的管理相对滞后、馆员本身的问题、人才流失严重、人才结构不合理等因素,提出了医院图书馆人力资源管理的新理念,阐述了实施科学人才管理、人才培训、队伍建设以及建立绩效考核制度、岗位聘用制度、人才政策体系等建议。

  17. Minimal Resources with Maximum Impact: Low-Cost and Sustainable Means for Library Promotions

    OpenAIRE

    Sagun, Karryl Kim A.

    2014-01-01

    While it is a generally shared fact around the globe that funding for library promotion (and in most cases, libraries in general) be rendered as a pressing concern, the fact remains that this challenge is truer in developing countries such as the Philippines. Librarians in charge of promoting the library can either choose to fight--defend their “rights” for a slice of the budget pie, or take flight--just do away with library promotion altogether. However, results of a low-cost and sustainable...

  18. Marketing the Virtual Library

    Science.gov (United States)

    Fagan, Jody Condit

    2009-01-01

    Far more people are familiar with their local public or college library facility than their library's website and online resources. In fact, according to a recent survey, 96% of Americans said they had visited a library in person, but less than one-third have visited their online library. Since everyone agrees that online library resources are…

  19. Herpesvirus BACs: Past, Present, and Future

    OpenAIRE

    Charles Warden; Qiyi Tang; Hua Zhu

    2011-01-01

    The herpesviridae are a large family of DNA viruses with large and complicated genomes. Genetic manipulation and the generation of recombinant viruses have been extremely difficult. However, herpesvirus bacterial artificial chromosomes (BACs) that were developed approximately 10 years ago have become useful and powerful genetic tools for generating recombinant viruses to study the biology and pathogenesis of herpesviruses. For example, BAC-directed deletion mutants are commonly used to determ...

  20. News from the Library: Online particle physics information: a unique compilation of information resources in particle physics

    CERN Multimedia

    CERN Library

    2012-01-01

    Are you looking for some specific information in particle physics? For example, the main literature databases, data repositories or laboratories...   Just go to the Libary's Online Particle Physics Information page. There you'll find a wide selection of relevant information, as well as resources in particle physics and related areas. The collection covers all aspects of the discipline - in addition to traditional scientific information resources you can find, for example, a selection of relevant blogs and art websites. This webpage is an extended and regularly updated version of the chapter on Online Particle Physics Information in the Review of Particle Properties. It is maintained by the CERN Library team which welcomes suggestions for additions and updates: library.desk@cern.ch.  

  1. Toolkit Approach to Integrating Library Resources into the Learning Management System

    Science.gov (United States)

    Black, Elizabeth L.

    2008-01-01

    As use of learning management systems (LMS) increases, it is essential that librarians are there. Ohio State University Libraries took a toolkit approach to integrate library content in the LMS to facilitate creative and flexible interactions between librarians, students and faculty in Ohio State University's large and decentralized academic…

  2. A Century of Change: The Evolution of School Library Resources, 1915-2015

    Science.gov (United States)

    Lamb, Annette

    2015-01-01

    School libraries have been in existence since at least the eighth century. However, it wasn't until the twentieth century that the school library was seen primarily as "a source of enrichment for the curriculum, and a means of developing reading and study habits in the pupils" (Clyde 1981, 263). While the formats available and tools for…

  3. Factors Influencing Virtual Patron Satisfaction with Online Library Resources and Services

    Science.gov (United States)

    Tyler, Katherine; Hastings, Nancy B.

    2011-01-01

    College students are accessing virtual libraries whether they are on campus or learning from a distance. Academic institutions serving virtual patrons must remain focused on meeting the needs of those library users by continually examining their preferences, their searching behavior, and the information they seek. The purpose of this research was…

  4. Wikipedia Lover, Not a Hater: Harnessing Wikipedia to Increase the Discoverability of Library Resources

    Science.gov (United States)

    Elder, Danielle; Westbrook, R. Niccole; Reilly, Michele

    2012-01-01

    During the spring of 2010, the University of Houston Libraries Digital Services Department began an initiative to promote existing and upcoming collections in the University of Houston Digital Library and drive traffic to the online repository. Spurred by an OCLC report (De Rosa et al. 2005) that only two percent of college and university students…

  5. 454 sequencing of pooled BAC clones on chromosome 3H of barley

    Directory of Open Access Journals (Sweden)

    Yamaji Nami

    2011-05-01

    Full Text Available Abstract Background Genome sequencing of barley has been delayed due to its large genome size (ca. 5,000Mbp. Among the fast sequencing systems, 454 liquid phase pyrosequencing provides the longest reads and is the most promising method for BAC clones. Here we report the results of pooled sequencing of BAC clones selected with ESTs genetically mapped to chromosome 3H. Results We sequenced pooled barley BAC clones using a 454 parallel genome sequencer. A PCR screening system based on primer sets derived from genetically mapped ESTs on chromosome 3H was used for clone selection in a BAC library developed from cultivar "Haruna Nijo". The DNA samples of 10 or 20 BAC clones were pooled and used for shotgun library development. The homology between contig sequences generated in each pooled library and mapped EST sequences was studied. The number of contigs assigned on chromosome 3H was 372. Their lengths ranged from 1,230 bp to 58,322 bp with an average 14,891 bp. Of these contigs, 240 showed homology and colinearity with the genome sequence of rice chromosome 1. A contig annotation browser supplemented with query search by unique sequence or genetic map position was developed. The identified contigs can be annotated with barley cDNAs and reference sequences on the browser. Homology analysis of these contigs with rice genes indicated that 1,239 rice genes can be assigned to barley contigs by the simple comparison of sequence lengths in both species. Of these genes, 492 are assigned to rice chromosome 1. Conclusions We demonstrate the efficiency of sequencing gene rich regions from barley chromosome 3H, with special reference to syntenic relationships with rice chromosome 1.

  6. What do academic libraries have to do with Open Educational Resources? Theme : Long term sustainability of open education projects first steps to start up

    OpenAIRE

    Robertson, R. John

    2010-01-01

    This paper will discuss the possible roles of academic libraries in promoting, supporting, and sustaining institutional Open Educational Resource initiatives. It will note areas in which libraries or librarians have skills and knowledge that intersect with some of the needs of academic staff and students as they use and release OERs. It will also present the results of a brief survey of the views of some OER initiatives on the current and potential role of academic libraries.

  7. Overlooked resources: the place of the library in visual science research.

    Science.gov (United States)

    Morgan, L

    1979-03-01

    Effective research should be based on familiarity with what has already been accomplished in the field. The best place to look for this information is a library, since even the most widely read professional may miss much that is relevant. Libraries not only provide access to their own holdings but assist the investigator by means of computerized literature searches and the use of indexes, abstracts, and other forms of printed reference material. Efficient use of library facilities can aid research and facilitate preparation of grant proposals. PMID:389055

  8. Combining resources, combining forces: regionalizing hospital library services in a large statewide health system.

    Science.gov (United States)

    Martin, Heather J; Delawska-Elliott, Basia

    2015-01-01

    After a reduction in full-time equivalents, 2 libraries in large teaching hospitals and 2 libraries in small community hospitals in a western US statewide health system saw opportunity for expansion through a regional reorganization. Despite a loss of 2/3 of the professional staff and a budgetary decrease of 27% over the previous 3 years, the libraries were able to grow business, usage, awareness, and collections through organizational innovation and improved efficiency. This paper describes the experience--including process, challenges, and lessons learned--of an organizational shift to regionalized services, collections, and staffing. Insights from this process may help similar organizations going through restructuring. PMID:25552945

  9. Experimental study on target treatment of nasopharyngeal carcinoma using 188Re-BAC5 combined with PYM-BAC5

    International Nuclear Information System (INIS)

    Objective: Combination of radiation and chemical agents was supposed to have better effect on nasopharyngeal cancer. This study compared the in vitro inhibiting effects of 188Re-monoclonal anti- body (BACs) with or without pingyangmycin conjugated BACs (PYM-BAC5) on nasopharyngeal carcinoma cells (CNE2). Methods: 188Re-BAC5 was prepared using 2-mereaptoethanol (ME), citric acid and tartaric acid as reducing and transchelation agents. Oxidized dextran T-40 (polyaldehyde dextran, PAD ) was used to conjugate PYM to BAC5. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenylte-trazolium bromide (MTT) method was applied to assess anti-tumor effects of 188Re-BAC5 and PYM-BAC5 on CNE2 cells. Results: The labeling efficiency of 188Re-BACs was 92%-95% with a radioactive concentration of 5254 kBq/ml. The composition of PYM-BAC5 was m (PYM): m (BAC5)=1: 0.526, or n (PYM): n(BAC5)=1:51. The inhibition effects of each treatment regime were in a dose dependent manner. The 50% inhibition doses (IC50) of 188Re-BAC5, 188Re-mIgG and 188ReO4- were 264, 1062 and 882 kBq/ml, respectively. The IC50 of the PYM-BAC5 and free PYM were 10.07 and 63.60 μg/ml, respectively. Combining 188Re-BAC5 and PYM-BACs reduced the IC50 of 1'88Re-BAC5 and PYM-BACs down to 32.1 kBq/ml and 1.70 μg/ml. Conclusions: It was proved that targeted therapy had better anti-tumor effect. The combination of targeted radiation and chemical agents (188Re-BAC5 + PYM-BAC5) obtained the best therapeutic effect on cultured CNE2 cells. (authors)

  10. Defining an open access resource strategy for research libraries: Part Ⅰ—The coming main streamlization of open access resources?

    Institute of Scientific and Technical Information of China (English)

    Xiaolin; ZHANG; Lin; LI; Xiwen; LIU; Yan; ZENG

    2011-01-01

    Open access(OA)resources are becoming a main part of scholarly information.The paper summarizes the fast growth of OA journals and OA repositories,the outstanding OA journal impact increase,the rise of mega-OA journals like PLoS ONE,the overwhelming support of research communities to open access of publicly funded research,and the quick building up of OA support structures.These forces work together to push scholarly communications toward a turning point where OA resources occupy a major and increasing share of scholarly research resources and when management of OA resources becomes a strategic,yet unfamiliar,and potentially disruptive task for research libraries.This paper is composed of two parts.The first part will focus on the development of OA resources,while the second part will analyze the challenges in management of OA resources and develop an OA resource strategy exemplified by some of NSL’s initiatives.

  11. GENSAT BAC Cre-recombinase driver lines to study the functional organization of cerebral cortical and basal ganglia circuits

    OpenAIRE

    Gerfen, Charles R.; Paletzki, Ronald; Heintz, Nathaniel

    2013-01-01

    Recent development of molecular genetic techniques are rapidly advancing understanding of the functional role of brain circuits in behavior. Critical to this approach is the ability to target specific neuron populations and circuits. The collection of over 250 BAC Cre-recombinase driver lines produced by the GENSAT project provides a resource for such studies. Here we provide characterization of GENSAT BAC-Cre driver lines with expression in specific neuroanatomical pathways within the cerebr...

  12. A hybrid BAC physical map of potato: a framework for sequencing a heterozygous genome

    Directory of Open Access Journals (Sweden)

    de Boer Jan M

    2011-12-01

    Full Text Available Abstract Background Potato is the world's third most important food crop, yet cultivar improvement and genomic research in general remain difficult because of the heterozygous and tetraploid nature of its genome. The development of physical map resources that can facilitate genomic analyses in potato has so far been very limited. Here we present the methods of construction and the general statistics of the first two genome-wide BAC physical maps of potato, which were made from the heterozygous diploid clone RH89-039-16 (RH. Results First, a gel electrophoresis-based physical map was made by AFLP fingerprinting of 64478 BAC clones, which were aligned into 4150 contigs with an estimated total length of 1361 Mb. Screening of BAC pools, followed by the KeyMaps in silico anchoring procedure, identified 1725 AFLP markers in the physical map, and 1252 BAC contigs were anchored the ultradense potato genetic map. A second, sequence-tag-based physical map was constructed from 65919 whole genome profiling (WGP BAC fingerprints and these were aligned into 3601 BAC contigs spanning 1396 Mb. The 39733 BAC clones that overlap between both physical maps provided anchors to 1127 contigs in the WGP physical map, and reduced the number of contigs to around 2800 in each map separately. Both physical maps were 1.64 times longer than the 850 Mb potato genome. Genome heterozygosity and incomplete merging of BAC contigs are two factors that can explain this map inflation. The contig information of both physical maps was united in a single table that describes hybrid potato physical map. Conclusions The AFLP physical map has already been used by the Potato Genome Sequencing Consortium for sequencing 10% of the heterozygous genome of clone RH on a BAC-by-BAC basis. By layering a new WGP physical map on top of the AFLP physical map, a genetically anchored genome-wide framework of 322434 sequence tags has been created. This reference framework can be used for anchoring and

  13. Resources

    Science.gov (United States)

    ... palate - resources Colon cancer - resources Cystic fibrosis - resources Depression - resources Diabetes - resources Digestive disease - resources Drug abuse - resources Eating disorders - resources Elder care - resources Epilepsy - resources Family troubles - ...

  14. Resources

    Science.gov (United States)

    ... Depression - resources Diabetes - resources Digestive disease - resources Drug abuse - resources Eating disorders - resources Elder care - resources Epilepsy - resources Family troubles - resources Gastrointestinal disorders - resources Hearing impairment - resources ...

  15. Development of the BAC Physical Maps of Wheat Chromosome 6B for Its Genomic Sequencing

    OpenAIRE

    Kobayashi, A.; Katagiri, S.; Karasawa, W.; Takumi, S.; Doležel, J. (Jaroslav); Ogihara, Y.; Handa, H.

    2015-01-01

    For a purpose of better understanding the genome structure of wheat and accelerating the development of DNA markers for gene isolations and breeding, the Japanese research group, as a member of The International Wheat Genome Sequencing Consortium, is now conducting the physical mapping and genomic sequencing of wheat chromosome 6B of ‘Chinese Spring’ (CS). BAC libraries were constructed respectively using the short and long arm-specific DNAs extracted from the flow-sorted chromosome 6BS and 6...

  16. Construction of the Bac-to-Bac System of Bombyx mori Nucleopolyhedroviru

    Institute of Scientific and Technical Information of China (English)

    Jin-shan HUANG; Bi-fang HAO; Xiu-lian SUN; Fei DENG; Hua-lin WANG; Zhi-hong HU

    2007-01-01

    To construct the Bac-to-Bac expression system of Bombyx mori nucleopolyhedrovirus (BmNPV), a transfer vector was constructed which contained an Escherichia coli (E. coli) mini-F replicon and a lacZ: attTN7: lacZ cassette within the upstream and downstream regions of the BmNPV polyhedrin gene. B. mori larvae were cotransfected with wild-type BmNPV genomic DNA and the transfer vector through subcutaneous injection to generate recombinant viruses by homologous recombination in vivo. The genomic DNA of budded viruses extracted from the hemolymph of the transfected larvae was used to transform E. coli DH10B. Recombinant bacmids were screened by kanamycin resistance, PCR and restriction enzyme (REN) digestion. One of the bacmid colonies, BmBacJS13, which had similar REN profiles to that of wild-type BmNPV, was selected for further research. To investigate the infectivity of BmBacJS13, the polyhedrin gene was introduced into the bacmid and the resultant recombinant (BmBacJS13-ph) was transfected to BmN cells. The budded viruses were collected from the supernatant of the transfected cells and used for infecting BmN cells. Growth curve analysis indicated that BmBacJS13-ph had a similar growth curve to that of wild-type BmNPV. Bio-assays indicated that BmBacJS13-ph was also infectious to B. mori larvae.

  17. 浅谈高校图书馆人力的开发和利用%Discussion on the Development and Utilization of Human Resources in University Library

    Institute of Scientific and Technical Information of China (English)

    李红

    2011-01-01

    本文分析了目前我国图书馆人力资源管理的现状及存在的问题,阐述了图书馆应构建人力资源管理战略,推动图书馆的可持续性发展.%This paper analyzes the current situation of human resource management of library and existing problems, elaborates that the library should build up human resources management strategy and promote the sustainable development of library.

  18. Strategic planning and policy for collection development of e-resources to satisfy user requirements : a case study of J R D Tata Memorial Library

    OpenAIRE

    Venkadesan, S.; Jagannath, Uma; Puttabasavaiah,

    2004-01-01

    The library environment is currently undergoing a rapid and dynamic revolution leading to new generation of libraries with an emphasis on e-resources. On one side, there is an increasing demand for good library collection in terms of large amount of data/information and on the other hand, the publishing media is striving hard to support this demand at lightning speed by way of e-publication as well as online access. As a result, a large number of e-resources are published on all subject areas...

  19. Physical mapping in large genomes: accelerating anchoring of BAC contigs to genetic maps through in silico analysis.

    Science.gov (United States)

    Paux, Etienne; Legeai, Fabrice; Guilhot, Nicolas; Adam-Blondon, Anne-Françoise; Alaux, Michaël; Salse, Jérôme; Sourdille, Pierre; Leroy, Philippe; Feuillet, Catherine

    2008-02-01

    Anchored physical maps represent essential frameworks for map-based cloning, comparative genomics studies, and genome sequencing projects. High throughput anchoring can be achieved by polymerase chain reaction (PCR) screening of bacterial artificial chromosome (BAC) library pools with molecular markers. However, for large genomes such as wheat, the development of high dimension pools and the number of reactions that need to be performed can be extremely large making the screening laborious and costly. To improve the cost efficiency of anchoring in such large genomes, we have developed a new software named Elephant (electronic physical map anchoring tool) that combines BAC contig information generated by FingerPrinted Contig with results of BAC library pools screening to identify BAC addresses with a minimal amount of PCR reactions. Elephant was evaluated during the construction of a physical map of chromosome 3B of hexaploid wheat. Results show that a one dimensional pool screening can be sufficient to anchor a BAC contig while reducing the number of PCR by 384-fold thereby demonstrating that Elephant is an efficient and cost-effective tool to support physical mapping in large genomes. PMID:18038165

  20. Hiro Hirai's BAC-FISH Protocol

    OpenAIRE

    sprotocols

    2014-01-01

    Author: Schistosoma Genome Network ### Overview This protocol is a modification of the standard FISH protocol published by Hiro Hirai and Phil LoVerde in Parasitology Today (1995, 11(8) p 310-314) that has been optimised for use with BAC probes and should be read in conjunction with the published protocol. This protocol uses the BIOPRIME reaction kit from GibcoBRL to prepare biotin-labelled BAC DNA which is detected using FITC-Avidin (Vector Labs, DCS grade). Reagents from other ...

  1. A novel method for increasing the transformation efficiency of Escherichia coli-application forbacterial artificial chromosome library construction.

    OpenAIRE

    Zhu, H; Dean, R.A.

    1999-01-01

    Bacterial artificial chromosome (BAC) libraries play a pivotal role in genomics studies. A crucial step in BAC library construction is the transformation of Escherichia coli by electroporation. Absolute efficiency (cfu/microgram DNA) is affected by a number of factors including the topological form and treatment of DNA samples. Here we report a simple new protocol using tRNA assisted precipitation that increased transformation efficiency by 70-fold for BAC ligations and up to 400-fold for pla...

  2. Defining an open access resource strategy for research libraries:Part Ⅲ—The strategies and practices of National Science Library

    Institute of Scientific and Technical Information of China (English)

    Xiaolin; ZHANG; Xiwen; LIU; Lin; LI; Yan; ZENG; Li-Ping; KU

    2012-01-01

    Purpose:This paper describes the strategies and practices of National Science Library(NSL),Chinese Academy of Sciences(CAS)in promoting open access(OA)and developing OA resources.Design/methodology/approach:Multi-facet frameworks are devised to guide the development of OA strategies and practices.Key OA initiatives are briefly described along the main aspects of the strategies as they contribute to implementation of the OA strategies.Findings:NSL defined its role as the Chief OA Officer for CAS and a key OA promoter for China.Accordingly,NSL has engaged in multiple fronts of promoting OA,including development of OA strategies for CAS,establishment of itself as an OA knowledge&promotion center,development of the CAS IR system,and support for OA publishing by CAS authors.Research limitations:OA is still evolving,so are the strategies and practices as many actions are experimental and explorative in nature.Open books,open data,and open educational resources are yet to be covered.Comparative studies are needed,so is the evidence-based impact analysis.Practical implications:Institutions can adopt,adapt,or compare with examples and lessons learned are described here.Originality/value:The multi-faceted frameworks,working principles,and lessons learned are based on NSL’s practices which can be valuable to the overall OA development.

  3. Electronic Resources and Mission Creep: Reorganizing the Library for the Twenty-First Century

    Science.gov (United States)

    Stachokas, George

    2009-01-01

    The position of electronic resources librarian was created to serve as a specialist in the negotiation of license agreements for electronic resources, but mission creep has added more functions to the routine work of electronic resources such as cataloging, gathering information for collection development, and technical support. As electronic…

  4. Distribution and uses of legume DNA clone resources

    International Nuclear Information System (INIS)

    Since 1990, my lab has developed and distributed various DNA clone resources for the legumes. In the first several years, the focus was on members of the tropical genus, Vigna, including the widely cultivated species, mungbean (V. radiata) and cowpea (V. unguiculata). Both of these grain legumes play key roles in agriculture in developing countries of Asia (mungbean) and Africa (cowpea). Moreover, because there is substantial genome conservation among legumes, these genetic resources have also been utilized by a wide range of researchers in other crop species. In 1997, my lab began to focus on the development and distribution of a new generation of DNA clone resources; Bacterial Artificial Chromosomes (BAC). A library of these clones was constructed in soybean (Glycine max) the most important legume species worldwide in terms of economic value. Again, the library has become a valuable resource for the legume research community and has been widely used in studies of legume genomics. (author)

  5. Transposon-mediated BAC transgenesis in zebrafish and mice

    OpenAIRE

    Sumiyama Kenta; Suster Maximiliano L; Kawakami Koichi

    2009-01-01

    Abstract Background Bacterial artificial chromosomes (BACs) are among the most widely used tools for studies of gene regulation and function in model vertebrates, yet methods for predictable delivery of BAC transgenes to the genome are currently limited. This is because BAC transgenes are usually microinjected as naked DNA into fertilized eggs and are known to integrate as multi-copy concatamers in the genome. Although conventional methods for BAC transgenesis have been very fruitful, complem...

  6. A Spectrum of Interoperability: The Site for Science Prototype for the NSDL; Re-Inventing the Wheel? Standards, Interoperability and Digital Cultural Content; Preservation Risk Management for Web Resources: Virtual Remote Control in Cornell's Project Prism; Safekeeping: A Cooperative Approach to Building a Digital Preservation Resource; Object Persistence and Availability in Digital Libraries; Illinois Digital Cultural Heritage Community-Collaborative Interactions among Libraries, Museums and Elementary Schools.

    Science.gov (United States)

    Arms, William Y.; Hillmann, Diane; Lagoze, Carl; Krafft, Dean; Marisa, Richard; Saylor, John; Terizzi, Carol; Van de Sompel, Herbert; Gill, Tony; Miller, Paul; Kenney, Anne R.; McGovern, Nancy Y.; Botticelli, Peter; Entlich, Richard; Payette, Sandra; Berthon, Hilary; Thomas, Susan; Webb, Colin; Nelson, Michael L.; Allen, B. Danette; Bennett, Nuala A.; Sandore, Beth; Pianfetti, Evangeline S.

    2002-01-01

    Discusses digital libraries, including interoperability, metadata, and international standards; Web resource preservation efforts at Cornell University; digital preservation at the National Library of Australia; object persistence and availability; collaboration among libraries, museums and elementary schools; Asian digital libraries; and a Web…

  7. 100 Colleges Sign Up with Google to Speed Access to Library Resources

    Science.gov (United States)

    Young, Jeffrey R.

    2005-01-01

    More than 100 colleges and universities have arranged to give people using the Google Scholar search engine on their campuses more-direct access to library materials. Google Scholar is a free tool that searches scholarly materials on the Web and in academic databases. The new arrangements essentially let Google know which online databases the…

  8. College Libraries and Resource Sharing: Testing a Compact Disc Union Catalog.

    Science.gov (United States)

    Townley, Charles T.

    1992-01-01

    Presents results of an evaluation of C.D. Cat, a CD-ROM union catalog developed by the Associated College Libraries of Central Pennsylvania consortium. Outcomes are reported in the areas of bibliographic quality, user evaluation, public relations strategies, bibliographic instruction, and guidelines for continuing operations. Recommendations are…

  9. Library Catalogue of Aboriginal Resources = Ouvrages Autochtones la Bibliothique. Aboriginal Peoples Collection.

    Science.gov (United States)

    Ministry of the Solicitor General, Ottawa (Ontario).

    This bibliography lists all books, reports, and audio and video cassettes about Canada natives and the criminal justice system located in the Ministry of the Solicitor General's Library in Ottawa (Canada). Section 1 lists authors, titles, and series in a single alphabetical sequence. Section 2 lists the entries by subject. Major subjects include…

  10. A first generation BAC-based physical map of the Asian seabass (Lates calcarifer.

    Directory of Open Access Journals (Sweden)

    Jun Hong Xia

    Full Text Available BACKGROUND: The Asian seabass (Lates calcarifer is an important marine foodfish species in Southeast Asia and Australia. Genetic improvement of this species has been achieved to some extent through selective breeding programs since 1990s. Several genomic tools such as DNA markers, a linkage map, cDNA and BAC libraries have been developed to assist selective breeding. A physical map is still lacking, although it is essential for positional cloning of genes located in quantitative trait loci (QTL and assembly of whole genome sequences. METHODOLOGY/PRINCIPAL FINDINGS: A genome-wide physical map of the Asian seabass was constructed by restriction fingerprinting of 38,208 BAC clones with SNaPshot HICF FPC technique. A total of 30,454 were assembled into 2,865 contigs. The physical length of the assembled contigs summed up to 665 Mb. Analyses of some contigs using different methods demonstrated the reliability of the assembly. CONCLUSIONS/SIGNIFICANCE: The present physical map is the first physical map for Asian seabass. This physical map will facilitate the fine mapping of QTL for economically important traits and the positional cloning of genes located in QTL. It will also be useful for the whole genome sequencing and assembly. Detailed information about BAC-contigs and BAC clones are available upon request.

  11. Immunotoxin BAC5-CT treated nasopharyngeal carcinoma

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    In this study, immunotoxin (IT) was prepared by conjugating BAC5 and CT with SPDP. The effects of IT on NPC and its mechanisms were explored using double labeled with radioactive nuclides, immunography and electron microscope technique in vivo and in vitro. The specific concentration of BAC5 in the tumor area showed. The radioactivity rate of tumor/nontumor (T/NT) was up to 10.26. IT had cytotoxic effects both on the cultured CNE-2 cell line and tumor multicell spheroides. In vivo, the preliminary result indicated that IT also had a inhibitory action on the nude mice models bearing human NPC (Reported in another article). Under electron microscope, the necrosis and apoptosis of tumor cells were found. The membranes of most tumor cells were found intacted not or corrosined, some of them had the character of apoptosis, including reduce of tumor cells membrane villi, condensation of cytoplasm and pyknosis or cleavage of nuclear. There were many of apoptosis bodies, which were occasionally phagocytosed by tumor cells. The infiltration of immunocytoes in tumor tissue could be seen. The results indicated that BAC5 can specifically combine with NPC cells and BAC5-CT has the inhibitory effect on NPC in vitro and in vivo, mechanism of which may be related to the effects that ‘warhead' CT dissolute the membrane of tumor cells directly, or/and IT promote the infiltration of immunocytoes so as to induce the apoptosis of tumor cell.

  12. Resources Integration Strategies for "985" University Libraries%“985”高校图书馆资源整合策略研究

    Institute of Scientific and Technical Information of China (English)

    沈艳芳; 肖鹏

    2012-01-01

    以11所“985”高校图书馆为样本,通过非介入式调查(包括图书馆实地考察、网站调研、图书馆发现系统调查)对各个图书馆资源整合的现状和问题进行调研,并采取访谈法获取这些高校图书馆领导层对资源整合的策略、态度和看法。对“985”高校图书馆资源整合的系统梳理对我国其他高校图书馆资源整合工作具有很强的指导意义。%Taking eleven "985" university libraries as samples, the authors find out the current status of the integration of library resources and the existing problems by using non-reactive investigation, and then probe their strategies, attitudes and thoughts regarding to integration of library resources by in-depth interviewing with these libraries' leaders. Systematic summary of the integration of library resources has the guiding significance for those university libraries who are working on integration of library resources.

  13. Network Based Educational Environment How Libraries and Librarians Become Organizers of Knowledge Access and Resources

    CERN Document Server

    Pettenati, M C; Pettenati, Corrado

    2000-01-01

    In this paper we will highlight some important issues which will influence the redefinition of roles and duties of libraries and librarians in a networked based educational environment. Although librarians will also keep their traditional roles of faculty support services as well as reference service and research assistance, we identify the participation in the instructional design process, the support in the evaluation, development and use of a proper authoring system and the customization of information access, as being the domains where libraries and librarians should mainly involve themselves in the next future and make profit of their expertise in information and knowledge organization in order to properly and effectively support the institutions in the use of Information Technology in education.

  14. Research Workshop 3: Search Methodology and Resources, Including Iranian National Library of Medicine

    OpenAIRE

    F. Karimi PharmD

    2009-01-01

    "nAims: Terminating this workshop, participants are expected to be able to: "nFind the medical databases on Internet; "nBecome familiar with different types of search techniques in medical databases; "nFinding articles in medical databases. "nWorkshop Framework: In this workshop, using Internet, participants will become familiar with search engines and databases abilities, including Iranian National Library of Medicine (INLM), to conduct a search in different items. &...

  15. Electronic Resources Management and Long Term Preservation (Is the library a growing organism?)

    OpenAIRE

    Giordano, Tommaso

    2007-01-01

    Digital preservation is a vast and complex issue which involves many aspects and areas of expertise. This paper is rather limited in scope and seeks to develop considerations concerning three questions: the first investigates how the problem of archiving and preservation of electronic journals is perceived by academic libraries, while the second concerns the organizational practices implemented in this area; I conclude with a comparison between the traditional approach and the emerging models...

  16. Research Workshop 3: Search Methodology and Resources, Including Iranian National Library of Medicine

    Directory of Open Access Journals (Sweden)

    F. Karimi PharmD

    2009-01-01

    Full Text Available "nAims: Terminating this workshop, participants are expected to be able to: "nFind the medical databases on Internet; "nBecome familiar with different types of search techniques in medical databases; "nFinding articles in medical databases. "nWorkshop Framework: In this workshop, using Internet, participants will become familiar with search engines and databases abilities, including Iranian National Library of Medicine (INLM, to conduct a search in different items.  

  17. An ultra-dense library resource for rapid deconvolution of mutations that cause phenotypes in Escherichia coli

    Science.gov (United States)

    Nehring, Ralf B.; Gu, Franklin; Lin, Hsin-Yu; Gibson, Janet L.; Blythe, Martin J.; Wilson, Ray; Bravo Núñez, María Angélica; Hastings, P. J.; Louis, Edward J.; Frisch, Ryan L.; Hu, James C.; Rosenberg, Susan M.

    2016-01-01

    With the wide availability of whole-genome sequencing (WGS), genetic mapping has become the rate-limiting step, inhibiting unbiased forward genetics in even the most tractable model organisms. We introduce a rapid deconvolution resource and method for untagged causative mutations after mutagenesis, screens, and WGS in Escherichia coli. We created Deconvoluter—ordered libraries with selectable insertions every 50 kb in the E. coli genome. The Deconvoluter method uses these for replacement of untagged mutations in the genome using a phage-P1-based gene-replacement strategy. We validate the Deconvoluter resource by deconvolution of 17 of 17 phenotype-altering mutations from a screen of N-ethyl-N-nitrosourea-induced mutants. The Deconvoluter resource permits rapid unbiased screens and gene/function identification and will enable exploration of functions of essential genes and undiscovered genes/sites/alleles not represented in existing deletion collections. This resource for unbiased forward-genetic screens with mapping-by-sequencing (‘forward genomics’) demonstrates a strategy that could similarly enable rapid screens in many other microbes. PMID:26578563

  18. Discussion on the Resources Integration after Normal University Library Amalgamation%师范院校图书馆合并后资源整合探讨

    Institute of Scientific and Technical Information of China (English)

    李爽

    2012-01-01

    本文从高校图书馆信息管理系统的合并、藏书的剔旧工作、建立并完善“学科馆员”制度等方面,对师范院校图书馆合并后资源整合进行了探讨。%In this paper, the resources integration after normal university library amalgamation is discussed from the following three aspects: the amalgamation of university library information management system, the weeding of library collection, as well as the establishment and perfection of subject librarian system.

  19. The Use of Quality Benchmarking in Assessing Web Resources for the Dermatology Virtual Branch Library of the National electronic Library for Health (NeLH)

    OpenAIRE

    Boulos, MN Kamel; Roudsari, AV; Gordon, C.; Gray, JA Muir

    2001-01-01

    Background In 1998, the U.K. National Health Service Information for Health Strategy proposed the implementation of a National electronic Library for Health to provide clinicians, healthcare managers and planners, patients and the public with easy, round the clock access to high quality, up-to-date electronic information on health and healthcare. The Virtual Branch Libraries are among the most important components of the National electronic Library for Health . They aim at creating online kno...

  20. Cloud Storage Mode of University Library Digital Resources%高校图书馆数字资源的云存储模式探讨

    Institute of Scientific and Technical Information of China (English)

    付晓红; 莫筱梅

    2012-01-01

    分析了传统存储模式下高校图书馆数字资源存储存在的问题,结合云存储的优势,讨论了应用云存储模式存储高校图书馆数字资源,提出并分析了两种具体的高校图书馆数字资源云存储模式。%This paper analyzes the problems of the storage of university libraries digital resources under traditional storage mode,discusses the storage of university libraries digital resources which apply cloud storage mode based on the advantage of cloud storage,proposes and analyzes two specific cloud storage modes of university libraries digital resource.

  1. A resource for CERN community: the Bookshop in the Central Library

    CERN Multimedia

    2008-01-01

    If you are looking for technical and scientific books on topics such as IT, Radiation Protection, Detectors and Experimental Techniques, Project Management, or simply for a good read, why not check whether the CERN Bookshop has it in stock? You will be surprised by its coverage in IT (150 titles) and physics (300 titles). In total the Bookshop sells around 500 titles from around 15 publishing houses. However, the offer is not limited to these publishers – the Bookshop staff are ready to order any title that exists in print. In addition, we welcome suggestions from the user community for new titles to be added to the stock. The title list - which is integrated into the CERN library catalogue – is available from http://cdsweb.cern.ch/collection/CERN Bookshop This Bookshop is located in the Central Library, Building 52 1-052 and is open on weekdays from 8.30 a.m. to 5.30 p.m. It can be contacted by e-mail (bookshop@cern.ch). CERN us...

  2. The complexity of Rhipicephalus (Boophilus microplus genome characterised through detailed analysis of two BAC clones

    Directory of Open Access Journals (Sweden)

    Valle Manuel

    2011-07-01

    Full Text Available Abstract Background Rhipicephalus (Boophilus microplus (Rmi a major cattle ectoparasite and tick borne disease vector, impacts on animal welfare and industry productivity. In arthropod research there is an absence of a complete Chelicerate genome, which includes ticks, mites, spiders, scorpions and crustaceans. Model arthropod genomes such as Drosophila and Anopheles are too taxonomically distant for a reference in tick genomic sequence analysis. This study focuses on the de-novo assembly of two R. microplus BAC sequences from the understudied R microplus genome. Based on available R. microplus sequenced resources and comparative analysis, tick genomic structure and functional predictions identify complex gene structures and genomic targets expressed during tick-cattle interaction. Results In our BAC analyses we have assembled, using the correct positioning of BAC end sequences and transcript sequences, two challenging genomic regions. Cot DNA fractions compared to the BAC sequences confirmed a highly repetitive BAC sequence BM-012-E08 and a low repetitive BAC sequence BM-005-G14 which was gene rich and contained short interspersed elements (SINEs. Based directly on the BAC and Cot data comparisons, the genome wide frequency of the SINE Ruka element was estimated. Using a conservative approach to the assembly of the highly repetitive BM-012-E08, the sequence was de-convoluted into three repeat units, each unit containing an 18S, 5.8S and 28S ribosomal RNA (rRNA encoding gene sequence (rDNA, related internal transcribed spacer and complex intergenic region. In the low repetitive BM-005-G14, a novel gene complex was found between to 2 genes on the same strand. Nested in the second intron of a large 9 Kb papilin gene was a helicase gene. This helicase overlapped in two exonic regions with the papilin. Both these genes were shown expressed in different tick life stage important in ectoparasite interaction with the host. Tick specific sequence

  3. Libraries for users services in academic libraries

    CERN Document Server

    Alvite, Luisa

    2010-01-01

    This book reviews the quality and evolution of academic library services. It revises service trends offered by academic libraries and the challenge of enhancing traditional ones such as: catalogues, repositories and digital collections, learning resources centres, virtual reference services, information literacy and 2.0 tools.studies the role of the university library in the new educational environment of higher educationrethinks libraries in academic contextredefines roles for academic libraries

  4. Use and Cost of Electronic Resources in Central Library of Ferdowsi University Based on E-metrics

    Directory of Open Access Journals (Sweden)

    Mohammad Reza Davarpanah

    2012-07-01

    Full Text Available The purpose of this study was to investigate the usage of electronic journals in Ferdowsi University, Iran based on e-metrics. The paper also aimed to emphasize the analysis of cost-benefit and the correlation between the journal impact factors and the usage data. In this study experiences of Ferdowsi University library on licensing and usage of electronic resources was evaluated by providing a cost-benefit analysis based on the cost and usage statistics of electronic resources. Vendor-provided data were also compared with local usage data. The usage data were collected by tracking web-based access locally, and by collecting vender-provided usage data. The data sources were one-year of vendor-supplied e-resource usage data such as Ebsco, Elsevier, Proquest, Emerald, Oxford and Springer and local usage data collected from the Ferdowsi university web server. The study found that actual usage values differ for vendor-provided data and local usage data. Elsevier has got the highest usage degree in searches, sessions and downloads. Statistics also showed that a small number of journals satisfy significant amount of use while the majority of journals were used less frequent and some were never used at all. The users preferred the PDF rather than HTML format. The data in subject profile suggested that the provided e-resources were best suited to certain subjects. There was no correlation between IF and electronic journal use. Monitoring the usage of e-resources gained increasing importance for acquisition policy and budget decisions. The article provided information about local metrics for the six surveyed vendors/publishers, e.g. usage trends, requests per package, cost per use as related to the scientific specialty of the university.

  5. 数字图书馆资源共建共享的探讨%Probe into Digital Library's Resources Co-construction and Sharing

    Institute of Scientific and Technical Information of China (English)

    王雅睿

    2015-01-01

    This paper analyzes the advantages of digital library's resources sharing,and puts forward some strategies for promoting digital library's resources co-construction and sharing, which include the technology strategy, talent strategy, legal strategy and industrial layout strategy.%分析了数字图书馆资源共享的优势,提出了促进数字图书馆资源共建共享的策略,包括技术策略、人才策略、法律策略、产业布局策略.

  6. Future Libraries: Dreams, Madness, & Reality.

    Science.gov (United States)

    Crawford, Walt; Gorman, Michael

    Policymakers and library administrators are being drawn to the idea of the "virtual library" and the "library without walls," the webs of electronic resources that supposedly will displace books, physical libraries, and most library staff, and are believing the virtual library to be imminent, adequate, and cost-effective. This book describes the…

  7. 纸质图书是图书馆的核心资源%Paper Books : Core Resources in Library

    Institute of Scientific and Technical Information of China (English)

    郑丽君

    2012-01-01

    The rise of the electronic books brings certain impact to the paper books,but the impact is far from subversive.Due to the paper books giving the readers the best reading,as well as the advantage of preservation,it is still the core resources of libraries.%电子图书的崛起给纸质图书带来一定的冲击,但这种冲击远没有达到颠覆性的强度。由于纸质图书带给读者最佳的阅读,纸质图书具有保存的优越性,因而纸质图书是图书馆的核心资源。

  8. Comparative analysis of catfish BAC end sequences with the zebrafish genome

    Directory of Open Access Journals (Sweden)

    Abernathy Jason

    2009-12-01

    Full Text Available Abstract Background Comparative mapping is a powerful tool to transfer genomic information from sequenced genomes to closely related species for which whole genome sequence data are not yet available. However, such an approach is still very limited in catfish, the most important aquaculture species in the United States. This project was initiated to generate additional BAC end sequences and demonstrate their applications in comparative mapping in catfish. Results We reported the generation of 43,000 BAC end sequences and their applications for comparative genome analysis in catfish. Using these and the additional 20,000 existing BAC end sequences as a resource along with linkage mapping and existing physical map, conserved syntenic regions were identified between the catfish and zebrafish genomes. A total of 10,943 catfish BAC end sequences (17.3% had significant BLAST hits to the zebrafish genome (cutoff value ≤ e-5, of which 3,221 were unique gene hits, providing a platform for comparative mapping based on locations of these genes in catfish and zebrafish. Genetic linkage mapping of microsatellites associated with contigs allowed identification of large conserved genomic segments and construction of super scaffolds. Conclusion BAC end sequences and their associated polymorphic markers are great resources for comparative genome analysis in catfish. Highly conserved chromosomal regions were identified to exist between catfish and zebrafish. However, it appears that the level of conservation at local genomic regions are high while a high level of chromosomal shuffling and rearrangements exist between catfish and zebrafish genomes. Orthologous regions established through comparative analysis should facilitate both structural and functional genome analysis in catfish.

  9. "You Just Type in What You Are Looking for": Undergraduates' Use of Library Resources vs. Wikipedia

    Science.gov (United States)

    Colon-Aguirre, Monica; Fleming-May, Rachel A.

    2012-01-01

    This study presents findings from one-on-one interviews with 21 undergraduate students at a large public research university in the southeastern United States. While the preliminary focus of the study was to be students' opinions about and use of Wikipedia as a resource for course-related research, many of the interviews evolved into discussion…

  10. Open and Editable: Exploring Library Engagement in Open Educational Resource Adoption, Adaptation and Authoring

    Science.gov (United States)

    Walz, Anita R.

    2015-01-01

    Open Educational Resources (OER) have saved students millions of dollars in textbook costs and greatly expanded access to a wide variety of educational materials for countless numbers of students and life-long learners. OER have also saved teachers time and effort by allowing them to reuse, modify, and build on materials developed by other…

  11. The Open Course Library: Using Open Educational Resources to Improve Community College Access

    Science.gov (United States)

    Goodwin, Mary Ann Lund

    2011-01-01

    Community colleges are committed to meeting the educational needs of the communities they serve and they have increased access to higher education by offering new and innovative services to students often unable to attend traditional baccalaureate institutions. An innovation known as Open Educational Resources (OER) promises to make college more…

  12. Recommendations for protecting National Library of Medicine Computing and Networking Resources

    Energy Technology Data Exchange (ETDEWEB)

    Feingold, R.

    1994-11-01

    Protecting Information Technology (IT) involves a number of interrelated factors. These include mission, available resources, technologies, existing policies and procedures, internal culture, contemporary threats, and strategic enterprise direction. In the face of this formidable list, a structured approach provides cost effective actions that allow the organization to manage its risks. We face fundamental challenges that will persist for at least the next several years. It is difficult if not impossible to precisely quantify risk. IT threats and vulnerabilities change rapidly and continually. Limited organizational resources combined with mission restraints-such as availability and connectivity requirements-will insure that most systems will not be absolutely secure (if such security were even possible). In short, there is no technical (or administrative) {open_quotes}silver bullet.{close_quotes} Protection is employing a stratified series of recommendations, matching protection levels against information sensitivities. Adaptive and flexible risk management is the key to effective protection of IT resources. The cost of the protection must be kept less than the expected loss, and one must take into account that an adversary will not expend more to attack a resource than the value of its compromise to that adversary. Notwithstanding the difficulty if not impossibility to precisely quantify risk, the aforementioned allows us to avoid the trap of choosing a course of action simply because {open_quotes}it`s safer{close_quotes} or ignoring an area because no one had explored its potential risk. Recommendations for protecting IT resources begins with discussing contemporary threats and vulnerabilities, and then procedures from general to specific preventive measures. From a risk management perspective, it is imperative to understand that today, the vast majority of threats are against UNIX hosts connected to the Internet.

  13. Digital Libraries and Educational Resources: the AquaRing Semantic Approach

    Directory of Open Access Journals (Sweden)

    Gianni Vercelli

    2010-03-01

    Full Text Available Large amounts of scientific digital contents are nowadays held by scientific institutions which collect, produce and store information valuable for dissemination, work, study and research. In this context, the development of the web and learning technologies has brought new opportunities for teachers and learners to retrieve and share pedagogical objects. This paper introduces the use of a semantic approach developed within the EC funded AquaRing project with the aim of improving access to the vast amount of digital content concerning the aquatic environment and its resources, as well as supporting enhanced education and informal learning in this specific domain. In order to achieve these goals, a semantic framework and an educational ontology were developed and implemented. Both were used to support the indexing of learning resources and to provide several educational services to end-users (especially children, students, parents and teachers.

  14. Targeted isolation, sequence assembly and characterization of two white spruce (Picea glauca BAC clones for terpenoid synthase and cytochrome P450 genes involved in conifer defence reveal insights into a conifer genome

    Directory of Open Access Journals (Sweden)

    Ritland Carol

    2009-08-01

    Full Text Available Abstract Background Conifers are a large group of gymnosperm trees which are separated from the angiosperms by more than 300 million years of independent evolution. Conifer genomes are extremely large and contain considerable amounts of repetitive DNA. Currently, conifer sequence resources exist predominantly as expressed sequence tags (ESTs and full-length (FLcDNAs. There is no genome sequence available for a conifer or any other gymnosperm. Conifer defence-related genes often group into large families with closely related members. The goals of this study are to assess the feasibility of targeted isolation and sequence assembly of conifer BAC clones containing specific genes from two large gene families, and to characterize large segments of genomic DNA sequence for the first time from a conifer. Results We used a PCR-based approach to identify BAC clones for two target genes, a terpene synthase (3-carene synthase; 3CAR and a cytochrome P450 (CYP720B4 from a non-arrayed genomic BAC library of white spruce (Picea glauca. Shotgun genomic fragments isolated from the BAC clones were sequenced to a depth of 15.6- and 16.0-fold coverage, respectively. Assembly and manual curation yielded sequence scaffolds of 172 kbp (3CAR and 94 kbp (CYP720B4 long. Inspection of the genomic sequences revealed the intron-exon structures, the putative promoter regions and putative cis-regulatory elements of these genes. Sequences related to transposable elements (TEs, high complexity repeats and simple repeats were prevalent and comprised approximately 40% of the sequenced genomic DNA. An in silico simulation of the effect of sequencing depth on the quality of the sequence assembly provides direction for future efforts of conifer genome sequencing. Conclusion We report the first targeted cloning, sequencing, assembly, and annotation of large segments of genomic DNA from a conifer. We demonstrate that genomic BAC clones for individual members of multi-member gene

  15. Fishing for function: zebrafish BAC transgenics for functional genomics

    OpenAIRE

    Chatterjee, Sumantra; Lufkin, Thomas

    2011-01-01

    Transgenics using bacterial artificial chromosomes (BACs) offers a great opportunity to look at gene regulation in a developing embryo. The modified BAC containing a reporter inserted just before the translational start site of the gene of interest allows for the visualization of spatio-temporal gene expression. Though this method has been used in the mouse model extensively, its utility in zebrafish studies is relatively new. This review aims to look at the utility of making BAC transgenics ...

  16. 醫學圖書館網路資源合作館藏發展 Internet Resources Cooperative Collection Development in Medical Library

    Directory of Open Access Journals (Sweden)

    Shwu-jiun Wang

    1999-06-01

    Full Text Available 無Internet has been well-developed and utilized, collection developments for both periodicals and databases and how to share the resources among libraries should be handled and organized by a national institution, such as National Central Library or Science& Technology Information Center. Then other libraries can take the advantages of Internet to collect and organize the valuable information by topics and subjects. It will not only utilize the virtual resources, save time for reference librarians but also help users to locate the information much easier and faster. As a side benefit, the traffic of Internet will also be decreased. Therefore under the present situation of shortage of manpower, high price of periodicals and databases, how to utilize and organize the resources from the Internet has become a possible way to achieve the purposes of collection developments and sharing resources. This article will present few models of medical libraries in the U.S. and European countries as our reference.

  17. Scholars' Satisfaction with Digital Library Collection and Gaps in the Provision of Effective Information Resources and Services: A Pakistani Perspective

    Science.gov (United States)

    Khan, Amjid; Ahmed, Shamshad; Masrek, Mohamad Noorman

    2014-01-01

    The study aims to explore the researchers' satisfaction with digital library resources and services and how they improved the research culture in Pakistani universities. A descriptive survey method was employed to achieve objectives of this study. Using stratified random sampling, for this survey we selected 14 public sector universities of…

  18. Expanding the functional utility of DOE family libraries through cooperative agreements: Implications for copyright law, fair use, combined purchasing power, and shared resources

    Energy Technology Data Exchange (ETDEWEB)

    Fisher, W.A.

    1989-04-01

    This paper will raise broad-based issues which impact on resource management. The maximization of assets and technology have given libraries unique and positive choices to improve research productivity. The economics power of mass purchasing, the accoutrements of technology and approaching copyright law in the spirit as well as the letter of the law, can be a boon for our profession.

  19. Making Higher Education More Affordable, One Course Reading at a Time: Academic Libraries as Key Advocates for Open Access Textbooks and Educational Resources

    Science.gov (United States)

    Okamoto, Karen

    2013-01-01

    Open access textbooks (OATs) and educational resources (OERs) are being lauded as a viable alternative to costly print textbooks. Some academic libraries are joining the OER movement by creating guides to open repositories. Others are promoting OATs and OERs, reviewing them, and even helping to create them. This article analyzes how academic…

  20. 云存储模式下图书馆数字资源建设%Library Digital Resources Construction Based on Cloud Storage Mode

    Institute of Scientific and Technical Information of China (English)

    曾祥文

    2011-01-01

    随着图书馆数字资源总量的快速增加,传统的存储方式在面对海量数据存储的需求上,存在着容量和性能的瓶颈。云存储作为一种新型的存储服务模式,为图书馆的海量数字资源建设提供了一种新的选择。在分析图书馆数字资源建设需求、介绍云存储概念的基础上,对基于云存储模式下图书馆数字资源的建设进行了探讨。%With the rapid increase in quantity of the library digital resources,there is capacity and performance of bottleneck when the traditional way of storage in the face of mass storage requirements.As a new storage service mode,cloud storage provides a new choice for the amount of library digital resources construction.Based on the analysis of library digital resources construction demand and the introduction of the concept of cloud storage,this paper discussed the library digital resources construction based on the cloud storage mode.

  1. The Data Model Resource Book A Library of Universal Data Models for All Enterprises

    CERN Document Server

    Silverston, Len

    2008-01-01

    A quick and reliable way to build proven databases for core business functionsIndustry experts raved about The Data Model Resource Book when it was first published in March 1997 because it provided a simple, cost-effective way to design databases for core business functions. Len Silverston has now revised and updated the hugely successful First Edition, while adding a companion volume to take care of more specific requirements of different businesses. Each volume is accompanied by a CD-ROM, which is sold separately. Each CD-ROM provides powerful design templates discussed in the books in a rea

  2. 圖書館事業專欄/Marketing of Electronic Information Resources: A Case of The J.D. Rockefeller Research Library, Egerton University/Nerisa Kamar

    OpenAIRE

    Nerisa Kamar

    2008-01-01

    This paper gives a brief overview of electronic information resources and services offered by The J.D. Rockefeller Research Library at Egerton University and the marketing of these resources. The paper examines the various reasons for marketing electronic information resources, with emphasis on the various, and illustrates marketing strategies used by J.D Rockefeller Research library towards effective utilization of the available resources in supporting research, teaching and learnin...

  3. Generation of BAC transgenic epithelial organoids.

    Directory of Open Access Journals (Sweden)

    Gerald Schwank

    Full Text Available Under previously developed culture conditions, mouse and human intestinal epithelia can be cultured and expanded over long periods. These so-called organoids recapitulate the three-dimensional architecture of the gut epithelium, and consist of all major intestinal cell types. One key advantage of these ex vivo cultures is their accessibility to live imaging. So far the establishment of transgenic fluorescent reporter organoids has required the generation of transgenic mice, a laborious and time-consuming process, which cannot be extended to human cultures. Here we present a transfection protocol that enables the generation of recombinant mouse and human reporter organoids using BAC (bacterial artificial chromosome technology.

  4. A dense genetic linkage map for common carp and its integration with a BAC-based physical map.

    Directory of Open Access Journals (Sweden)

    Lan Zhao

    Full Text Available BACKGROUND: Common carp (Cyprinus carpio is one of the most important aquaculture species with an annual global production of 3.4 million metric tons. It is also an important ornamental species as well as an important model species for aquaculture research. To improve the economically important traits of this fish, a number of genomic resources and genetic tools have been developed, including several genetic maps and a bacterial artificial chromosome (BAC-based physical map. However, integrated genetic and physical maps are not available to study quantitative trait loci (QTL and assist with fine mapping, positional cloning and whole genome sequencing and assembly. The objective of this study was to integrate the currently available BAC-based physical and genetic maps. RESULTS: The genetic map was updated with 592 novel markers, including 312 BAC-anchored microsatellites and 130 SNP markers, and contained 1,209 genetic markers on 50 linkage groups, spanning 3,565.9 cM in the common carp genome. An integrated genetic and physical map of the common carp genome was then constructed, which was composed of 463 physical map contigs and 88 single BACs. Combined lengths of the contigs and single BACs covered a physical length of 498.75 Mb, or around 30% of the common carp genome. Comparative analysis between common carp and zebrafish genomes was performed based on the integrated map, providing more insights into the common carp specific whole genome duplication and segmental rearrangements in the genome. CONCLUSION: We integrated a BAC-based physical map to a genetic linkage map of common carp by anchoring BAC-associated genetic markers. The density of the genetic linkage map was significantly increased. The integrated map provides a tool for both genetic and genomic studies of common carp, which will help us to understand the genomic architecture of common carp and facilitate fine mapping and positional cloning of economically important traits for

  5. Olefin Isomerization Regiochemistries during Tandem Action of BacA and BacB on Prephenate in Bacilysin Biosynthesis†

    OpenAIRE

    Parker, Jared B.; Walsh, Christopher T.

    2012-01-01

    BacA and BacB, the first two enzymes of the bacilysin pathway, convert prephenate to an exocylic regioisomer of dihydrohydroxyphenylpyruvate (ex-H2HPP) on the way to the epoxycyclohexanone warhead in the dipeptide antibiotic, bacilysin. BacA decarboxylates prephenate without aromatization, converting the 1,4-diene in prephenate to the endocyclic 1,3 diene in Δ4Δ8-dihydrohydroxyphenylpyruvate (en-H2HPP). BacB then performs an allylic isomerization to bring the diene into conjugation with the 2...

  6. HUMAN RESOURCE DEVELOPMENT IN ACADEMIC LIBRARIES IN THE AGE OF INFORMATION TECHNOLOGY AND NEED OF TRAINING : AN OVERVIEW.

    OpenAIRE

    KANADE MANJUSHA LAXMANRAO; SHYAMKANT J.DESHMUKH

    2013-01-01

    Development of any Nation is depends upon its quality of education. In this digital era libraries must adopt digital technologies. Academic library plays vital role in imparting education to its clients. Due to the rapid growth in the information generation and in varied forms, ever increasing information demands from users, now days libraries must convert their collection and services from traditional to the automated. With the help of computer and software, Libraries can provide right infor...

  7. Better Collaborative Working is Likely to Increase Uptake of Library Resources in a University Setting. A review of: Hightower, Barbara, Carolyn Rawl, and Michelle Schutt. “Collaborations for Delivering the Library to Students Through WebCT.” Reference Services Review 35.4 (2007: 541-51.

    Directory of Open Access Journals (Sweden)

    John Loy

    2008-06-01

    Full Text Available Objectives – To ascertain the extent to which university faculty members are integrating library resources within the WebCT course management system / managed learning environment. Also, to identify the reasons why faculty members are not integrating library resources within WebCT, and to explore their willingness to do so in future. Design – Case study with survey questionnaire and selected interview follow up.Setting – Urban campus of Auburn University in Montgomery, Alabama, United States of America.Subjects – One hundred and two members of faculty received a questionnaire. Five follow-up interviews were conducted.Methods – One hundred and two members of faculty with WebCT-supported courses received a questionnaire. They were asked to indicate if they linked to library resources, and if so which specific ones (e.g. library homepage, online catalogue, etc.. If they did not link to library resources they were asked to indicate the reason why not, either by selecting one or more of five pre-selected reasons, or by detailing their own. Follow-up interviews were conducted with five respondents, two of whom (Education and Nursing linked to library resources, and three of whom (from Business, Education and Science did not. Server log reports were also examined to identify how many students were entering library resources from WebCT.Main Results – The survey response rate was 28% (29 individuals, and of these Nursing and Sciences were the highest users with 7 individuals in each faculty. Of the 29 respondents only 7 (24% currently linked to library resources. Nursing were the biggest group with 10 links (38% of the total, Science with 6 (23% and 5 each from Liberal Arts and Education (19%. The resources chosen to link to were• Article databases (6• Library homepage (5• Ask a Librarian e-mail service(3• Net library e-book collection (3Six other library resources scored lower and two were not linked to at all. While few respondents

  8. Integrated library systems.

    OpenAIRE

    Goldstein, C M

    1983-01-01

    The development of integrated library systems is discussed. The four major discussion points are (1) initial efforts; (2) network resources; (3) minicomputer-based systems; and (4) beyond library automation. Four existing systems are cited as examples of current systems.

  9. Extensive conserved synteny of genes between the karyotypes of Manduca sexta and Bombyx mori revealed by BAC-FISH mapping.

    Directory of Open Access Journals (Sweden)

    Yuji Yasukochi

    Full Text Available BACKGROUND: Genome sequencing projects have been completed for several species representing four highly diverged holometabolous insect orders, Diptera, Hymenoptera, Coleoptera, and Lepidoptera. The striking evolutionary diversity of insects argues a need for efficient methods to apply genome information from such models to genetically uncharacterized species. Constructing conserved synteny maps plays a crucial role in this task. Here, we demonstrate the use of fluorescence in situ hybridization with bacterial artificial chromosome probes as a powerful tool for physical mapping of genes and comparative genome analysis in Lepidoptera, which have numerous and morphologically uniform holokinetic chromosomes. METHODOLOGY/PRINCIPAL FINDINGS: We isolated 214 clones containing 159 orthologs of well conserved single-copy genes of a sequenced lepidopteran model, the silkworm, Bombyx mori, from a BAC library of a sphingid with an unexplored genome, the tobacco hornworm, Manduca sexta. We then constructed a BAC-FISH karyotype identifying all 28 chromosomes of M. sexta by mapping 124 loci using the corresponding BAC clones. BAC probes from three M. sexta chromosomes also generated clear signals on the corresponding chromosomes of the convolvulus hawk moth, Agrius convolvuli, which belongs to the same subfamily, Sphinginae, as M. sexta. CONCLUSIONS/SIGNIFICANCE: Comparison of the M. sexta BAC physical map with the linkage map and genome sequence of B. mori pointed to extensive conserved synteny including conserved gene order in most chromosomes. Only a few rearrangements, including three inversions, three translocations, and two fission/fusion events were estimated to have occurred after the divergence of Bombycidae and Sphingidae. These results add to accumulating evidence for the stability of lepidopteran genomes. Generating signals on A. convolvuli chromosomes using heterologous M. sexta probes demonstrated that BAC-FISH with orthologous sequences can be

  10. 高校图书馆人力资源开发与管理探究%Study on Human Resources Development and Management in Academic Library --For library of Yangtze Normal University an e

    Institute of Scientific and Technical Information of China (English)

    刘晓莉

    2011-01-01

    Based on the state of human resources of Academic library, this article points out the problems of human resources management in libraries at present. Some suggestions, which includes librarian's inspection and training and the idea of human oriented, sho%文章就高校图书馆人力资源的现状进行分析,指出了存在的问题,提出了加强馆员的考核及培训和强化以人为本的理念是当前改善高校图书馆人力资源管理现状的重要举措。

  11. Sequencing of a QTL-rich region of the Theobroma cacao genome using pooled BACs and the identification of trait specific candidate genes

    Directory of Open Access Journals (Sweden)

    Blackmon Barbara P

    2011-07-01

    Full Text Available Abstract Background BAC-based physical maps provide for sequencing across an entire genome or a selected sub-genomic region of biological interest. Such a region can be approached with next-generation whole-genome sequencing and assembly as if it were an independent small genome. Using the minimum tiling path as a guide, specific BAC clones representing the prioritized genomic interval are selected, pooled, and used to prepare a sequencing library. Results This pooled BAC approach was taken to sequence and assemble a QTL-rich region, of ~3 Mbp and represented by twenty-seven BACs, on linkage group 5 of the Theobroma cacao cv. Matina 1-6 genome. Using various mixtures of read coverages from paired-end and linear 454 libraries, multiple assemblies of varied quality were generated. Quality was assessed by comparing the assembly of 454 reads with a subset of ten BACs individually sequenced and assembled using Sanger reads. A mixture of reads optimal for assembly was identified. We found, furthermore, that a quality assembly suitable for serving as a reference genome template could be obtained even with a reduced depth of sequencing coverage. Annotation of the resulting assembly revealed several genes potentially responsible for three T. cacao traits: black pod disease resistance, bean shape index, and pod weight. Conclusions Our results, as with other pooled BAC sequencing reports, suggest that pooling portions of a minimum tiling path derived from a BAC-based physical map is an effective method to target sub-genomic regions for sequencing. While we focused on a single QTL region, other QTL regions of importance could be similarly sequenced allowing for biological discovery to take place before a high quality whole-genome assembly is completed.

  12. Sports Education Library Information Services

    Institute of Scientific and Technical Information of China (English)

    许晓峰

    2014-01-01

    Library website, is the first window for readers to understand library information services. Sports Education academy library fully take advantage of homepage, combine open access resource searched with the library collections, after targeted collection, selection, sorting, processing, clustering or reorganization, establish a navigation system of open access resource of physical Sports Education.

  13. Genealogy Resources Construction in Guangzhou Library%广州图书馆的家谱资源建设

    Institute of Scientific and Technical Information of China (English)

    何映雯

    2014-01-01

    ABSTRACT:The genealogy, official history and chorography constitute the three pillars of Chinese historical documents, they supplement historical facts from different angles, making the historical appearance, time spirit and social custom get more real and more comprehensive presentation, and have an irreplaceable special function for the deep study of history, demography, sociology and other subjects. This paper expounds the significance of the construction of genealogy resources, and probes into the works that Guangzhou Library has done in aspects of genealogy resources’ collection, sorting, development, and service, etc.%家谱与正史、方志是构成中华历史文献的三大支柱,它们从不同角度补充史实,使历史面貌、时代精神和社会风尚得以更真实、更全面的呈现,对历史学、人口学及社会学等学科的深入研究具有不可替代的独特功能。阐述了建设家谱资源的意义,探讨了广州图书馆在家谱资源的征集、整理、开发及服务方面所做的工作。

  14. Study in Grey and White: Measuring the Impact of the 8Rs Canadian Library Human Resources Study

    Directory of Open Access Journals (Sweden)

    Allison Sivak

    2009-06-01

    Full Text Available Objective – To use the 8Rs Canadian Library Human Resources Study (the 8Rs Study as a test case to develop a model for assessing research impact in LIS. Methods – Three different methods of citation analysis which take into account the changing environment of scholarly communications. These include a ‚manual‛ method of locating citations to the 8Rs Study through a major LIS database, an enhanced-citation tool Google Scholar, and a general Google search to locate Study references in non-scholarly documents Results – The majority of references (82% were found using Google or Google Scholar; the remainder were located via LISA. Each method had strengths and limitations.Conclusion - In-depth citation analysis provides a promising method of understanding the reach of published research. This investigation’s findings suggest the need for improvements in LIS citation tools, as well as digital archiving practices to improve the accessibility of references for measuring research impact. The findings also suggest the merit of researchers and practitioners defining levels of research impact, which will assist researchers in the dissemination of their work.

  15. A collection of books from Bielany in the Jagiellonian Library. Security, maintenance and availability of resources. A notice on the progress of work, June 2011

    Directory of Open Access Journals (Sweden)

    Jacek Partyka

    2011-12-01

    Full Text Available In the autumn of 2009 the Jagiellonian Library became the depositary of a large, valuable and interesting collection of printed books from the library of the Camaldolese Monastery in Bielany (Krakow. The work on processing the collection began almost immediately after all the prints had been taken over by the Jagiellonian Library. Two groups of employees have been involved in the work, both from the Resource Processing Department and the Antique Books Department, in line with the natural division into modern and old prints. The Camaldolese collection from Bielany has been enclosed in the monastery and unavailable to most readers; now the very books which for many years have been the source of knowledge, assistance and cognitive tools to a narrow circle of monks may be accessed by all interested persons. In the next year, after basic processing of the collection, the books will create an extremely interesting research opportunity.

  16. Construction and Identification of Bacterial Artificial Chromosome Library for 0-613-2R in Upland Cotton

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    A bacterial artificial chromosome (BAC) library containing a large genomic DNA insert is an important tool for genome physical mapping, map-based cloning, and genome sequencing. To isolate genes via a map-based cloning strategy and to perform physical mapping of the cotton genome, a high-quality BAC library containing large cotton DNA inserts is needed. We have developed a BAC library of the restoring line 0-613-2R for isolating the fertility restorer (Rf1) gene and genomic research in cotton (Gossypium hirsutum L.). The BAC library contains 97 825 clones stored in 255 pieces of a 384-well microtiter plate. Random samples of BACs digested with the Notl enzyme indicated that the average insert size is approximately 130 kb, with a range of 80-275 kb,and 95.7% of the BAC clones in the library have an average insert size larger than 100 kb. Based on a cotton genome size of 2 250 Mb, library coverage is 5.7 x haploid genome equivalents. Four clones were selected randomly from the library to determine the stability of the BAC clones. There were no different fingerprints for 0 and 100 generations of each clone digested with Notl and Hindlll enzymes. Thus, the stability of a single BAC clone can be sustained at least for 100 generations. Eight simple sequence repeat (SSR) markers flanking the Rf1 gene were chosen to screen the BAC library by pool using PCR method and 25 positive clones were identified with 3.1 positive clones per SSR marker.

  17. There is a Relationship between Resource Expenditures and Reference Transactions in Academic Libraries. A Review of: Dubnjakovic, A. (2012. Electronic resource expenditure and the decline in reference transaction statistics in academic libraries. Journal of Academic Librarianship, 38(2, 94-100. doi:10.1016/j.acalib.2012.01.001

    Directory of Open Access Journals (Sweden)

    Annie M. Hughes

    2013-03-01

    Full Text Available Objective – To provide an analysis of the impact of expenditures on electronic resourcesand gate counts on the increase or decrease in reference transactions.Design – Analysis of results of existing survey data from the National Center for Educational Statistics (NCES 2006 Academic Library Survey(ALS.Setting – Academic libraries in the United States.Subjects – 3925 academic library respondents.Methods – The author chose to use survey data collected from the 2006 ALS conducted bythe NCES. The survey included data on various topics related to academic libraries, but in the case of this study, the author chose to analyze three of the 193 variables included. The three variables: electronic books expenditure, computer hardware and software, and expenditures on bibliographic utilities, were combined into one variable called electronic resource expenditure. Gate counts were also considered as a variable. Electronic resource expenditure was also split as a variable into three groups: low, medium, and high. Multiple regression analysis and general linear modeling, along with tests of reliability, were employed. Main Results – The author determined that low, medium, and high spenders with regard to electronic resources exhibited differences in gate counts, and gate counts have an effect on reference transactions in any given week. Gate counts tend to not have much of an effect on reference transactions for the higher spenders, and higher spenders tend to have a higher number of reference transactions overall. Low spenders have lower gate counts and also a lower amount of reference transactions.Conclusion – The findings from this study show that academic libraries spending more on electronic resources also tend to have an increase with regard to reference transactions. The author also concludes that library spaces are no longer the determining factor with regard to number of reference transactions. Spending more on electronic resources is

  18. 图书馆的特色资源建设研究%Research on the Construction of Library Characteristic Resources

    Institute of Scientific and Technical Information of China (English)

    陈玉梅

    2014-01-01

    图书馆的特色资源建设在近些年来成为热点词汇,面对当前我国图书馆特色资源建设面临的诸多弊病,本文通过成功的范例阐述了通过PDA、联邦式共享等方式对图书馆的特色资源建设的改革思路。%The construction of library characteristic resources has become a hot topic in recent years. Faced with the shortcomings of the current construction of library characteristic resources in China, this paper, baseds on successful examples, elaborated the reform ideas through the method of PDA and cooperative sharing.

  19. Directorio de consultores, recursos y sitios de Internet relacionados con bibliotecas mexicanas = Directory of consultants, resources & Internet sites relating to Mexican libraries

    OpenAIRE

    Abrams, William S.

    2006-01-01

    This guidebook was compiled for: 1) foreign librarians, 2) Mexican students enrolled in programs of study in the field of librarianship or in certificate programs in library science, 3) volunteers or those in practicum service, 4) paraprofessionals or the recently degreed Mexican librarian, 5) non-experts. This work, a bilingual annotated directory, contains basic information on a wide range of resources relevant to librarianship as presently practiced in Mexico: books, articles, useful web ...

  20. Web 2.0 as Catalyst: Virtually Reaching Out to Users and Connecting Them to Library Resources and Services

    Science.gov (United States)

    Xiao, Norah

    2008-01-01

    This article is based on services to library users in the area of chemistry at the Science and Engineering (S&E) Library of the University of Southern California (USC), to which I applied various new technologies as outreach approaches. Various Web 2.0 technologies such as a blog, tags, YouTube, RSS feeds, Instant Messaging, online presentation…

  1. Genomic insight into the common carp (Cyprinus carpio genome by sequencing analysis of BAC-end sequences

    Directory of Open Access Journals (Sweden)

    Wang Jintu

    2011-04-01

    Full Text Available Abstract Background Common carp is one of the most important aquaculture teleost fish in the world. Common carp and other closely related Cyprinidae species provide over 30% aquaculture production in the world. However, common carp genomic resources are still relatively underdeveloped. BAC end sequences (BES are important resources for genome research on BAC-anchored genetic marker development, linkage map and physical map integration, and whole genome sequence assembling and scaffolding. Result To develop such valuable resources in common carp (Cyprinus carpio, a total of 40,224 BAC clones were sequenced on both ends, generating 65,720 clean BES with an average read length of 647 bp after sequence processing, representing 42,522,168 bp or 2.5% of common carp genome. The first survey of common carp genome was conducted with various bioinformatics tools. The common carp genome contains over 17.3% of repetitive elements with GC content of 36.8% and 518 transposon ORFs. To identify and develop BAC-anchored microsatellite markers, a total of 13,581 microsatellites were detected from 10,355 BES. The coding region of 7,127 genes were recognized from 9,443 BES on 7,453 BACs, with 1,990 BACs have genes on both ends. To evaluate the similarity to the genome of closely related zebrafish, BES of common carp were aligned against zebrafish genome. A total of 39,335 BES of common carp have conserved homologs on zebrafish genome which demonstrated the high similarity between zebrafish and common carp genomes, indicating the feasibility of comparative mapping between zebrafish and common carp once we have physical map of common carp. Conclusion BAC end sequences are great resources for the first genome wide survey of common carp. The repetitive DNA was estimated to be approximate 28% of common carp genome, indicating the higher complexity of the genome. Comparative analysis had mapped around 40,000 BES to zebrafish genome and established over 3

  2. On the Cloud Storage Mode of University Library Digital Resources%高校图书馆数字资源云存储模式探究

    Institute of Scientific and Technical Information of China (English)

    施蓉蓉

    2012-01-01

    With the rapid increase in quantity of library digital resources,the drawbacks of traditional data storage model increasingly appear.As a new storage service mode,cloud storage provides a new choice for the amount of li-brary digital resources construction.This paper introduces some digital storage technologies,and discusses the application of cloud storage in university library digital resources storage.%随着图书馆数字资源总量的快速增加,传统的数据存储方式弊端日益显现。云存储作为一种新型的存储服务模式,为图书馆的海量数字资源建设提供了一种新的选择。在介绍数字存储技术的基础上,重点探讨了云存储在高校图书馆数字资源存储中的应用。

  3. Chromosome region-specific libraries for human genome analysis. Final progress report, 1 March 1991--28 February 1994

    Energy Technology Data Exchange (ETDEWEB)

    Kao, F.T.

    1994-04-01

    The objectives of this grant proposal include (1) development of a chromosome microdissection and PCR-mediated microcloning technology, (2) application of this microtechnology to the construction of region-specific libraries for human genome analysis. During this grant period, the authors have successfully developed this microtechnology and have applied it to the construction of microdissection libraries for the following chromosome regions: a whole chromosome 21 (21E), 2 region-specific libraries for the long arm of chromosome 2, 2q35-q37 (2Q1) and 2q33-q35 (2Q2), and 4 region-specific libraries for the entire short arm of chromosome 2, 2p23-p25 (2P1), 2p21-p23 (2P2), 2p14-p16 (wP3) and 2p11-p13 (2P4). In addition, 20--40 unique sequence microclones have been isolated and characterized for genomic studies. These region-specific libraries and the single-copy microclones from the library have been used as valuable resources for (1) isolating microsatellite probes in linkage analysis to further refine the disease locus; (2) isolating corresponding clones with large inserts, e.g. YAC, BAC, P1, cosmid and phage, to facilitate construction of contigs for high resolution physical mapping; and (3) isolating region-specific cDNA clones for use as candidate genes. These libraries are being deposited in the American Type Culture Collection (ATCC) for general distribution.

  4. Canadian Library Human Resources Short‐Term Supply and Demand Crisis Is Averted, But a Significant Long‐Term Crisis Must Be Addressed. A review of: 8Rs Research Team. The Future of Human Resources in Canadian Libraries February 2005. Edmonton, AB: University of Alberta. 21 February 2007 .

    OpenAIRE

    Julie McKenna

    2007-01-01

    Objective – To examine the human resources environment in Canadian libraries in order to assess readiness to accommodate change and to identify opportunities for human resources planning. The “8Rs” of the study were defined as recruitment, retirement, retention, remuneration, repatriation, rejuvenation, re‐accreditation, and restructuring.Design – This study was undertaken in three phases over nearly three years through the use a variety of methods including literature review, analyses of exi...

  5. Changing State Digital Libraries

    Science.gov (United States)

    Pappas, Marjorie L.

    2006-01-01

    Research has shown that state virtual or digital libraries are evolving into websites that are loaded with free resources, subscription databases, and instructional tools. In this article, the author explores these evolving libraries based on the following questions: (1) How user-friendly are the state digital libraries?; (2) How do state digital…

  6. Library news

    CERN Multimedia

    CERN Library

    2010-01-01

    The CERN Library has been providing electronic access to the "Techniques de l'Ingénieur" database for the past 8 months. As a reminder, this is a multidisciplinary database of over 4000 technical and scientific articles in French, covering a broad range of topics such as mechanical engineering, safety, electronics and the environment. In a few simple steps, you can create your own account, select the types of documents you are interested in and configure your settings so as to receive alerts when articles in your field of activity are published. You can now access this resource from outside CERN using the "remote access to electronic resources" service. Further information is available here. Direct access to the database. Remote access to electronic resources. If you have any questions or comments, don't hesitate to contact us at: library.desk@cern.ch.

  7. Construction of random sheared fosmid library from Chinese cabbage and its use for Brassica rapa genome sequencing project

    Institute of Scientific and Technical Information of China (English)

    Tae-Ho Park; Beom-Seok Park; Jin-A Kim; Joon Ki Hong; Mina Jin; Young-Joo Seol; Jeong-Hwan Mun

    2011-01-01

    As a part of the Multinational Genome Sequencing Project of Brassica rapa, linkage group R9 and R3 were sequenced using a bacterial artificial chromosome (BAC) by BAC strategy. The current physical contigs are expected to cover approximately 90% euchromatins of both chromosomes. As the project progresses, BAC selection for sequence extension becomes more limited because BAC libraries are restriction enzyme-specific. To support the project, a random sheared fosmid library was constructed. The library consists of 97536 clones with average insert size of approximately 40 kb corresponding to seven genome equivalents, assuming a Chinese cabbage genome size of 550 Mb. The library was screened with primers designed at the end of sequences of nine points of scaffold gaps where BAC clones cannot be selected to extend the physical contigs. The selected positive clones were end-sequenced to check the overlap between the fosmid clones and the adjacent BAC clones.Nine fosmid clones were selected and fully sequenced. The sequences revealed two completed gap filling and seven sequence extensions, which can be used for further selection of BAC clones confirming that the fosmid library will facilitate the sequence completion of B. rapa.

  8. A high-throughput strategy for screening of bacterial artificial chromosome libraries and anchoring of clones on a genetic map constructed with single nucleotide polymorphisms

    OpenAIRE

    Deal Karin R; Ma Yaqin; Xu Kenong; Luo Ming-Cheng; Nicolet Charles M; Dvorak Jan

    2009-01-01

    Abstract Background Current techniques of screening bacterial artificial chromosome (BAC) libraries for molecular markers during the construction of physical maps are slow, laborious and often assign multiple BAC contigs to a single locus on a genetic map. These limitations are the principal impediment in the construction of physical maps of large eukaryotic genomes. It is hypothesized that this impediment can be overcome by screening multidimensional pools of BAC clones using the highly para...

  9. A high-throughput strategy for screening of bacterial artificial chromosome libraries and anchoring of clones on a genetic map constructed with single nucleotide polymorphisms

    OpenAIRE

    Luo, Ming-Cheng; Xu, Kenong; Ma, Yaqin; Karin R Deal; Nicolet, Charles M.; Dvorak, Jan

    2009-01-01

    Background Current techniques of screening bacterial artificial chromosome (BAC) libraries for molecular markers during the construction of physical maps are slow, laborious and often assign multiple BAC contigs to a single locus on a genetic map. These limitations are the principal impediment in the construction of physical maps of large eukaryotic genomes. It is hypothesized that this impediment can be overcome by screening multidimensional pools of BAC clones using the highly parallel Illu...

  10. 高校图书馆数字资源保存政策分析%Analysis of Digital Resource Preservation Policies of University Libraries

    Institute of Scientific and Technical Information of China (English)

    寇爽

    2012-01-01

    University libraries play an important role in the digital resource preservation,so they should formulate reasonable and perfect long-term preservation policies to guide the digital resource preservation.Digital resource preservation policies of university libraries in American could make into comprehensive policies and specialized policies,these policies have same points: making sure the mission or purpose of the digital resource preservation should be consistent with the mission of the university,describing the information of preservation objects clearly,giving prominence to the position of the non-technical factors including financial support and employee,and indicating that they should establish cooperation mechanisms.Digital resource preservation activities start late in China,and digital resource preservation policies of university libraries are relatively less.Therefore,according to actual situations of China and the successful experience of foreign countries,university libraries should formulate digital resource preservation policies from the preservation mission,preservation subjects,preservation objects,preservation strategies,safeguard mechanisms,and cooperation mechanisms.%高校图书馆在数字资源保存方面发挥着重要作用,应制定合理、完善的长期保存政策来引导数字资源保存的实践。美国高校图书馆数字资源保存政策分为综合性政策和专门性政策,这些政策都明确表示数字资源保存的使命(目的)应与大学本身的使命一致,并明确描述了保存客体(对象)的相关信息,突出了财政支持和员工等非技术因素的地位,明确指出要建立合作机制。我国数字资源保存活动起步较晚,高校图书馆的数字资源保存政策相对较少。我国高校图书馆应根据实际情况,借鉴国外的成功经验,从保存使命、保存主体、保存客体、保存策略、保障机制、合作机制等方面着手制定数字资源保存政策。

  11. Remobilizing deleted piggyBac vector post-integration for transgene stability in silkworm.

    Science.gov (United States)

    Wang, Feng; Wang, Riyuan; Wang, Yuancheng; Xu, Hanfu; Yuan, Lin; Ding, Huan; Ma, Sanyuan; Zhou, You; Zhao, Ping; Xia, Qingyou

    2015-06-01

    Deletion of transposable elements post-genomic integration holds great promise for stability of the transgene in the host genome and has an essential role for the practical application of transgenic animals. In this study, a modified piggyBac vector that mediated deletion of the transposon sequence post-integration for transgene stability in the economically important silkworm Bombyx mori was constructed. The piggyBac vector architecture contains inversed terminal repeat sequences L1, L2 and R1, which can form L1/R1 and L2/R1 types of transposition cassettes. hsp70-PIG as the piggyBac transposase expression cassette for initial transposition, further remobilization and transgene stabilization test was transiently expressed in a helper vector or integrated into the modified vector to produce a transgenic silkworm. Shortening L2 increased the transformation frequency of L1/R1 into the silkworm genome compared to L2/R1. After the integration of L1/R1 into the genome, the remobilization of L2/R1 impaired the transposon structure and the resulting transgene linked with an impaired transposon was stable in the genome even in the presence of exogenously introduced transposase, whereas those flanked by the intact transposon were highly mobile in the genome. Our results demonstrated the feasibility of post-integration deletion of transposable elements to guarantee true transgene stabilization in silkworm. We suggest that the modified vector will be a useful resource for studies of transgenic silkworms and other piggyBac-transformed organisms. PMID:25589404

  12. Digital Reading and the Information Resource Development of Libraries%数字阅读与图书馆信息资源建设

    Institute of Scientific and Technical Information of China (English)

    肖希明; 曾粤亮

    2016-01-01

    数字阅读时代的到来给图书馆信息资源建设带来了前所未有的机遇与挑战。本文从数字阅读的普及环境、发展态势以及知识获取地位等方面论述数字阅读的趋势与常态;剖析了数字阅读的特点及其在资源结构、资源建设模式、资源采集方式及资源购置经费结构等方面对图书馆信息资源建设带来的影响;认为在数字阅读背景下,图书馆应当贯彻“虚实并重”的资源建设基本方针,积极为数字阅读提供条件,参与数字资源生产并提供优质数字资源,开发网络资源并引导数字阅读,积极推进公共数字文化资源整合。%Digital reading has brought unprecedented opportunities and chal enges for the development of library information resources. The article discusses the normal and trends of digital reading in terms of its popularity, development situation and the status of obtaining knowledge. It analyses the characteristics of digital reading and its impact on libraries from the perspective of the resource structure, resource development modes, resource acquisition methods and expenditure structure of resource al ocation. In the background of digital reading, libraries should lay equal stress on both virtual and physical resources, create conditions for readers, participate in the production of digital resources and provide services, develop network resources and offer guidance and actively promote the integration of public digital cultural resources.

  13. Customized USB flash drives used to promote library resources and services to first-year medical and dental students.

    Science.gov (United States)

    Matsoukas, Konstantina; Meret, Anca; Oliver, John T; Purcell, Michael

    2011-01-01

    In line with its institution's commitment to keep new student orientation/welcome events "green," Columbia University's Health Sciences Library (HSL) applied for a National Network of Libraries of Medicine Middle Atlantic Region (NN/LM MAR) Small Projects Award(1) in Spring/Summer 2009 to explore paperless modes of outreach. This article describes the project that resulted from this funding, whose purpose was to determine whether customized 1 GB USB flash drives are a good vehicle for distributing library promotional materials to incoming medical and dental students. This project gave HSL librarians the opportunity to connect with students in a way that had not been done before and to consider how these new students use/view the information the library produces. PMID:21534111

  14. A first generation BAC-based physical map of the rainbow trout genome

    Directory of Open Access Journals (Sweden)

    Thorgaard Gary H

    2009-10-01

    Full Text Available Abstract Background Rainbow trout (Oncorhynchus mykiss are the most-widely cultivated cold freshwater fish in the world and an important model species for many research areas. Coupling great interest in this species as a research model with the need for genetic improvement of aquaculture production efficiency traits justifies the continued development of genomics research resources. Many quantitative trait loci (QTL have been identified for production and life-history traits in rainbow trout. A bacterial artificial chromosome (BAC physical map is needed to facilitate fine mapping of QTL and the selection of positional candidate genes for incorporation in marker-assisted selection (MAS for improving rainbow trout aquaculture production. This resource will also facilitate efforts to obtain and assemble a whole-genome reference sequence for this species. Results The physical map was constructed from DNA fingerprinting of 192,096 BAC clones using the 4-color high-information content fingerprinting (HICF method. The clones were assembled into physical map contigs using the finger-printing contig (FPC program. The map is composed of 4,173 contigs and 9,379 singletons. The total number of unique fingerprinting fragments (consensus bands in contigs is 1,185,157, which corresponds to an estimated physical length of 2.0 Gb. The map assembly was validated by 1 comparison with probe hybridization results and agarose gel fingerprinting contigs; and 2 anchoring large contigs to the microsatellite-based genetic linkage map. Conclusion The production and validation of the first BAC physical map of the rainbow trout genome is described in this paper. We are currently integrating this map with the NCCCWA genetic map using more than 200 microsatellites isolated from BAC end sequences and by identifying BACs that harbor more than 300 previously mapped markers. The availability of an integrated physical and genetic map will enable detailed comparative genome

  15. 浅谈公共图书馆的人力资源开发与管理%On the Development and Management of Human Resources at Public Libraries

    Institute of Scientific and Technical Information of China (English)

    许迎霞

    2012-01-01

    提出了新世纪对图书馆员素质要求,图书馆应该在日常工作中开发人力资源,要加强人力资源管理,要优化人员结构,建立公平竞争机制,引入激励机制,强化考核手段,建立有效的培训机制,以图书馆文化感染人,制定合理、有效的人力资源开发与管理规则,以实现人力资源管理的科学化、规范化和民主化。%The paper according to the demand of quality of librarians in the new century, The author Proposed the library should develop the human resources in the routine work, Must strengthen the human resources management, must optimize the personnel structure, establishes the fair competition mechanism, the introduction drives the mecha- nism, the strengthened inspection method, establishes the effective training mechanism, by library culture infection person, formulation reasonable, effective human resources development and management rule, realizes the human resources management scientific style, the standardization and the democratization

  16. Challenges and Opportunities for Libraries in Pakistan

    OpenAIRE

    Shafiq UR, Rehman; Pervaiz, Ahmad

    2007-01-01

    Abstract: This paper, based on review of literature, observation, and informal conversations, discusses various challenges regarding finance, collection development, ICTs, human resources, library education, library association and research & development faced by library profession in Pakistan. The opportunities to meet these challenges have also been explored. Keywords: Library challenges and opportunities (Pakistan); Librarianship (Pakistan); Library issues; Library profession in Pa...

  17. School and Library Media. Introduction; The Uniform Computer Information Transactions Act (UCITA): More Critical for Educators than Copyright Law?; Redefining Professional Growth: New Attitudes, New Tools--A Case Study; Diversity in School Library Media Center Resources; Image-Text Relationships in Web Pages; Aiming for Effective Student Learning in Web-Based Courses: Insights from Student Experiences.

    Science.gov (United States)

    Fitzgerald, Mary Ann; Gregory, Vicki L.; Brock, Kathy; Bennett, Elizabeth; Chen, Shu-Hsien Lai; Marsh, Emily; Moore, Joi L.; Kim, Kyung-Sun; Esser, Linda R.

    2002-01-01

    Chapters in this section of "Educational Media and Technology Yearbook" examine important trends prominent in the landscape of the school library media profession in 2001. Themes include mandated educational reform; diversity in school library resources; communication through image-text juxtaposition in Web pages; and professional development and…

  18. Psychological Analysis of Public Library Readers in the Environment of Computer Network Resources%计算机网络资源环境下公共图书馆读者心理分析

    Institute of Scientific and Technical Information of China (English)

    梁佳

    2012-01-01

    Public libraries as a nonprofit cultural educational institution,is open for the public,all members of society who use the library resources,library services object.The Public Library of the nature of its readers with a wide range of social and mass characteristics.Understanding of computer network resources and the environment,public library readers psychology,master of readers of different ages tend to provide a basis for public library services.Public Library audience composition and reading motivation and purpose,read the psychological differences.Readers 'psychology,and the corresponding collection of literature data and network information resources services to meet readers' needs to improve the collection of documents and network resource utilization,of great significance for readers of different age groups.The computer network resources and the environment,public library readers psychological to make analysis,to further explore the computer network resources and the environment,how public libraries should better carry out the reader service work.%公共图书馆作为公益性文化教育机构,是面向社会公众开放的,凡是具有利用图书馆资源条件的一切社会成员,都是图书馆服务对象。公共图书馆的性质决定了它的读者具有广泛的社会性和群众性特点。了解计算机网络资源环境下公共图书馆的读者心理,掌握不同年龄读者的阅读倾向,能为公共图书馆读者服务工作提供依据。

  19. Analysis on University Libraries Digital Resources Construction and Service%浅析高校图书馆数字资源建设与服务

    Institute of Scientific and Technical Information of China (English)

    于娟

    2016-01-01

    As the fast development of information technology and internet, paper resources in libraries of colleges and universities have become unsatisifed with the fast development of knowledge and scientiifc efforts. Large amount of paper resources also bring large cost of maintenance. In this condition, digital resources construction with faster updating and lower cost of maintenance is becoming greatly important. Therefore how to well construct digital resources in universities and provide better service for readers has been an important theme to librarians. Only good digital library resources and services can relfect the excellence of digital resources and provide better services to readers.The paper analyzes university libraries digital resources construction and service.%随着信息技术和互联网的飞速发展,高校图书馆中纸质馆藏资源已经渐渐不能满足知识和科研成果突飞猛进的发展需求,同时大量的纸质馆藏资源也带来了较高的维护成本。在这种环境下,更新速度较快、维护成本较低的数字资源建设就显得尤为重要。因此,如何建设好高校数字资源、为广大读者提供更好的数字资源服务,是摆在广大图书馆工作者面前的一个重要课题。只有优质的馆藏数字资源和服务,才能充分体现数字资源的优越性,更好地服务于大众。文章分析了高校图书馆数字资源建设与服务。

  20. L’italiano alla prova del Bac

    Directory of Open Access Journals (Sweden)

    Giulia Massignan

    2013-07-01

    Full Text Available Il lavoro propone alcune attività didattiche di preparazione dell’esame orale di italiano previsto dal Baccalauréat del liceo francese. Tale prova d’esame è stata recentemente oggetto di una riforma ministeriale volta a dare maggior peso alla pratica orale. Dopo uno studio delle nuove modalità della prove d’esame e dei suoi contenuti, ci si concentrerà sull’analisi delle abilità orali necessarie al suo superamento e sugli elementi linguistici e comunicativi su cui porre l’attenzione. Sulla base di queste premesse teoriche e metodologiche, si presentano alcuni interventi didattici di preparazione all’esame: due attività per la prova di comprensione orale e due per la prova di produzione e interazione orale; in entrambi i casi, si insiste sull’importanza di proporre strategie d’ascolto e di parlato insegnabili, che diventino consuetudini attive e parte integrante del bagaglio comunicativo del discente. The italian exam in the FrenchBacThe paper proposes some educational activities to prepare for the oral Italian examination as part of the French Baccalauréat. This exam has recently been the subject of a ministerial reform aimed at giving more weight to the oral practice. After illustrating new examination methods and content, we will focus on the analysis of oral skills necessary for passing the exam and aspects of language and communication on which to focus. Based on these theoretical and methodological premises, some educational exam preparation activities will be presented. Two activities test listening comprehension and two focus on production and oral interaction. The stress is on the importance of proposing teach able listening and speaking strategies, so that they become active habits and integral parts of the communicative learner’s baggage.

  1. E-learning resource library model based on domain ontology%基于领域本体的电子学习资源库模型

    Institute of Scientific and Technical Information of China (English)

    张沪寅; 张铭洋; 李鑫

    2012-01-01

    随着电子学习系统快速的发展,电子学习资源呈现爆炸式的增长,如何有效地组织海量电子学习资源成为构建高效电子学习系统的重要因素.针对现有资源库在资源组织方面存在的不足,提出了一个基于领域知识本体的电子学习资源库检索模型,该模型利用领域知识来构建领域知识本体库并通过抽取电子学习资源元数据构建元数据库,通过映射关系完成对电子学习资源的语义组织,并在此基础之上构建一个语义检索模型,以有效地解决现有电子学习资源检索中丢失语义背景的问题,使检索结果在查全率、查准率方面有所提高,更加符合用户的需求.%With the rapid development of E-learning system, E-learning resources grow explosively. How to effectively organize E-learning resources is a key factor of constructing efficient E-learning system. Concerning the existing resources organization deficiency of E-learning resource library, this paper proposed an E-learning resource retrieval model based on domain ontology. This model built a domain knowledge library by making use of the domain knowledge and constructed E-learning resources metadata database by extracting resources metadata, realized semantic organization of E-learning resources through mapping relations, and constructed a semantic retrieval model on this basis, in order to effectively solve the problem of the loss of semantic background in the E-learning resource retrieving. The model has also enhanced the recall rate and the precision rate on the retrieval results, and it is more in line with the needs of the users.

  2. Paths of Discovery: Comparing the Search Effectiveness of EBSCO Discovery Service, Summon, Google Scholar, and Conventional Library Resources

    Science.gov (United States)

    Asher, Andrew D.; Duke, Lynda M.; Wilson, Suzanne

    2013-01-01

    In 2011, researchers at Bucknell University and Illinois Wesleyan University compared the search efficacy of Serial Solutions Summon, EBSCO Discovery Service, Google Scholar, and conventional library databases. Using a mixed-methods approach, qualitative and quantitative data were gathered on students' usage of these tools. Regardless of the…

  3. The first generation of a BAC-based physical map of Brassica rapa

    Directory of Open Access Journals (Sweden)

    Lee Soo

    2008-06-01

    Full Text Available Abstract Background The genus Brassica includes the most extensively cultivated vegetable crops worldwide. Investigation of the Brassica genome presents excellent challenges to study plant genome evolution and divergence of gene function associated with polyploidy and genome hybridization. A physical map of the B. rapa genome is a fundamental tool for analysis of Brassica "A" genome structure. Integration of a physical map with an existing genetic map by linking genetic markers and BAC clones in the sequencing pipeline provides a crucial resource for the ongoing genome sequencing effort and assembly of whole genome sequences. Results A genome-wide physical map of the B. rapa genome was constructed by the capillary electrophoresis-based fingerprinting of 67,468 Bacterial Artificial Chromosome (BAC clones using the five restriction enzyme SNaPshot technique. The clones were assembled into contigs by means of FPC v8.5.3. After contig validation and manual editing, the resulting contig assembly consists of 1,428 contigs and is estimated to span 717 Mb in physical length. This map provides 242 anchored contigs on 10 linkage groups to be served as seed points from which to continue bidirectional chromosome extension for genome sequencing. Conclusion The map reported here is the first physical map for Brassica "A" genome based on the High Information Content Fingerprinting (HICF technique. This physical map will serve as a fundamental genomic resource for accelerating genome sequencing, assembly of BAC sequences, and comparative genomics between Brassica genomes. The current build of the B. rapa physical map is available at the B. rapa Genome Project website for the user community.

  4. Completely Distinguishing Individual A-genome Chromosomes and Their Karyotyping Analysis by Multiple BAC-FISH

    Institute of Scientific and Technical Information of China (English)

    WANG Kai; GUO Wang-zhen; ZHANG Tian-zhen

    2008-01-01

    @@ Multiple BAC-FISH is a powerful tool for modern cytogenetic researching in both animals and plants.But in cotton,this technique is unavailable due to the high percentage of repetitive sequences.Here,we identified twenty BACs from more than fifty BACs,and successfully demonstrated the use of multiple BAC-FISH for cytogenetie research in a diploid cotton species,G.arboreum.The karyotyping should be a basic application of this technique,but the potential usage such as high-resolution physical mapping construction,assisting BAC-by-BAC sequencing will be invaluable.

  5. The Physical Location of Gm-2 and Gm-6 in O.officinalis with BAC-FISH Based on Comparative RFLP Map of Wild Rice and Cultivated Rice

    Institute of Scientific and Technical Information of China (English)

    QIN Rui; WEI Wen-hui; NING Shun-bin; JIN Wei-wei; HE Guang-cun; SONG Yun-chun

    2002-01-01

    Rice BAC library is used widely in rice genome research due to its distinctive advantages over other library systems. In this study, two rice BAC clones closely linked to rice gall midge resistance, Gm-2 and Gm-6, were in situ hybridized to Oryza officinalis chromosomes. They were located on the long arm of chromosome 4 with FL 72.33% and 77.10% respectively and their FL was consistent with the selective marker of rice, RG214 and RZ569. The frequency of signal detection was 61.2% and 59.5% respectively.Our study was based on comparative RFLP map of wild rice, O. officinalis, and cultivated rice, O. sativa.

  6. 高校图书馆数字资源建设刍议%The Discussion of the Construction of the University Library Digital Resource

    Institute of Scientific and Technical Information of China (English)

    王令祎

    2014-01-01

    Summary: the construction of the digital resource has become the most important part of University li-brary's literature resources construction, but there still has many Underlying reasons which put off the development of the construction of digital resources. This paper will discuss and show some ideas on six questions, which are the construction of digital resources, popularization of it, intellectual property protection problems, interlibrary coopera-tion, the construction standards of digital resources, and evaluation system of users.%数字资源建设已经成为高校图书馆文献资源建设的重要组成部分,但许多深层次原因阻碍着高校数字资源建设的脚步。本文从数字资源重复建设、推广普及度、知识产权保护问题、馆际协作、数字资源建设标准和使用评价系统六个方面进行探讨,为问题的解决提出一些思路。

  7. Library/vendor relationships

    CERN Document Server

    Brooks, Sam

    2014-01-01

    A view of the mutual dependence between libraries and vendorsAs technology advances, libraries are forced to reach beyond their own resources to find effective ways to maintain accuracy and superior service levels. Vendors provide databases and integrated library systems that perform those functions for profit. Library/Vendor Relationships examines the increasing cooperation in which libraries find they must participate in, and vice versa, with the vendors that provide system infrastructure and software. Expert contributors provide insights from all sides of this unique collaboration, offering

  8. Database Technology in Digital Libraries.

    Science.gov (United States)

    Preston, Carole; Lin, Binshan

    2002-01-01

    Reviews issues relating to database technology and digital libraries. Highlights include resource sharing; ongoing digital library projects; metadata; user interfaces; query processing; interoperability; data quality; copyright infringement; and managerial implications, including electronic versus printed materials, accessibility,…

  9. 当前高校图书馆人力资源管理问题研究%Study on Human Resource Management of Current University Library

    Institute of Scientific and Technical Information of China (English)

    刘宏

    2012-01-01

    Taking knowledge-based economy environment as the background, the thesis discussed the current status of human resources in university libraries and the problems, and made countermeasures and proposals for human resources optimization from the concept, planning, environment, configuration, training, performance appraisal, culture and other aspects.%以知识经济环境下为背景,讨论了当前高校图书馆人力资源现状及存在的问题,提出了从观念、规划、环境、配置、培训、绩效考核、文化等环节优化人力资源的对策建议.

  10. Canadian Library Human Resources Short‐Term Supply and Demand Crisis Is Averted, But a Significant Long‐Term Crisis Must Be Addressed. A review of: 8Rs Research Team. The Future of Human Resources in Canadian Libraries February 2005. Edmonton, AB: University of Alberta. 21 February 2007 .

    Directory of Open Access Journals (Sweden)

    Julie McKenna

    2007-03-01

    Full Text Available Objective – To examine the human resources environment in Canadian libraries in order to assess readiness to accommodate change and to identify opportunities for human resources planning. The “8Rs” of the study were defined as recruitment, retirement, retention, remuneration, repatriation, rejuvenation, re‐accreditation, and restructuring.Design – This study was undertaken in three phases over nearly three years through the use a variety of methods including literature review, analyses of existing data (Statistics Canada and library school graduate data, telephone interviews (with senior library administrators, focus groups (with representatives from Canadian Association of Research Libraries, Canadian Urban Libraries Council and Alberta Association of Library Technicians, print surveys (library institutions and web‐based surveys (of professional librarians and paraprofessional library staff.Setting – Canadian libraries that are not component branches of a system, and that employ professional librarians.Subjects – Stage I: 17 senior library administrators participated in telephone interviews and three focus groups were conducted. Stage II: Surveyed library administrators representing institutions. A multi‐stage stratified random sampling technique was used to ensure geographical representation from each of Canada’s provinces and territories. Full census participation was conducted for members of the Canadian Association of Research Libraries and the Canadian Urban Libraries Council. The print survey instrument was distributed to 1,357 subjects; 461 completed surveys were returned (response rate of 34% with results for the total sample accurate within plus or minus 3.8 per centage points, 95 times out of 100. Stage III: Surveyed professional librarians and paraprofessional staff. Multi‐stage random sampling was used to ensure representation of library staff from all library sectors and sufficient sub‐sample sizes. Of the

  11. Learners with Low Self-Efficacy for Information Literacy Rely on Library Resources Less Often But Are More Willing to Learn How to Use Them

    Directory of Open Access Journals (Sweden)

    Dominique Daniel

    2014-09-01

    Full Text Available Objectives – To determine whether there is a relationship between self-efficacy (i.e., confidence regarding information literacy skills and self-efficacy for distance learning; and to compare the use of electronic resources by high and low information literacy self-efficacy distance learners and their interest in learning more about searching. Design – Online survey. Setting – A small public university in the United States of America. Subjects – Undergraduate and graduate students enrolled in one or more online courses. Most respondents were in their twenties, 76% were female, 59% were undergraduates, and 69% were full time students. Methods – Students were asked six demographic questions, eight questions measuring their self-efficacy for information literacy, and four questions measuring their self-efficacy for online learning. All self-efficacy questions were adapted from previous studies and used a one to five Likert scale. The response rate was 6.2%. Correlational analysis was conducted to test the first two hypotheses (students who have higher self-efficacy for information seeking are more likely to have higher self-efficacy for online learning and for information manipulation. Descriptive analysis was used for the remaining hypotheses, to test whether students who have higher information literacy self-efficacy are more likely to have high library skills (hypothesis three and are more interested in learning about how to use library resources (hypothesis four. Among respondents high information literacy self-efficacy and low self-efficacy groups were distinguished, using the mean score of information literacy self-efficacy. Main Results – There was a significant correlation between self-efficacy for information seeking and self-efficacy for online learning (r = .27, as well as self-efficacy for information manipulation (r = .79. Students with high information seeking self-efficacy were more likely to use library databases (28

  12. 图情资源发现系统的研究与实现*%Research and Implementation of Libraries' Resource Discovery System

    Institute of Scientific and Technical Information of China (English)

    王灏; 张正锋; 冯巍

    2013-01-01

      资源发现系统是一种面向图书情报领域的垂直搜索引擎。文章从图情机构馆藏和服务的问题入手,介绍了资源发现系统的概念和技术路线,并以“中国学术搜索网”为例,介绍了该平台在资源发现服务中的总体设计、关键技术、核心功能和服务方式,旨在帮助图书馆等单位在产品技术选型和建设过程中提供可能的参考依据。%Resource discovery system is a kind of vertical search engine focused on library and information science. For the general problem in libraries and information centers, this article gave a brief introduction of the concept and main technologies. With the example of"Chinese Academic Search,"it has provided some practical details about the design, main function and service model of resource systems in order to provide suggestions for others' purchase or construction of discovery service if possible.

  13. Link Established Between LBGT-Friendly Campus Climate Index Scores and Web-Based Resources of Academic Libraries. A Review of: Ciszek, M. P. (2011. Out on the web: The relationship between campus climate and GLBT-related web-based resources in academic libraries. The Journal of Academic Librarianship, 37(5, 430-436. doi: 10.1016/j.acalib.2011.06.007

    Directory of Open Access Journals (Sweden)

    Kathleen Reed

    2013-03-01

    Full Text Available Objective – To explore whether academic institutions that score highly on the LGBT Friendly Campus Climate Index also have well-developed Web-based library resources to support GLBT (gay, lesbian, bisexual, transgender students.Design – Website analysis, percentage, and binary logistic regression analysis.Setting – Library websites of colleges and universities in four American geographic regions: Northeast, Midwest, South, and West.Subjects – There were 259 colleges and universities that participated in the 2010 LGBT-Friendly Campus Climate Index.Methods – The author visited the library websites of all institutions and surveyed available GLBT-related resources. The criteria for online resources included: 1 A research guide for GLBT studies or geared toward GLBT students, 2 An individual identified within the research guide as a contact for GLBT-related resources, and 3 A subscription to EBSCO’s GLBT Life database. Whether or not the academic libraries had the above resources was then analyzed with each institution’s score on the climate survey scale. The author controlled for geographical location, religious affiliation, and campus setting of the college or university.Main Results – There is a positive direct relationship between whether a library makes GLBT resources available on the Web and campus climate. However, only 25% of libraries surveyed published a research guide, 18% named a contact individual, and 31% subscribed to GLBT Life.Conclusion – While parent institutions commit to GLBT students by taking the LBGT-Friendly Campus Climate Index survey, academic libraries lag behind providing online resources for this community.

  14. Spectral library searching in proteomics.

    Science.gov (United States)

    Griss, Johannes

    2016-03-01

    Spectral library searching has become a mature method to identify tandem mass spectra in proteomics data analysis. This review provides a comprehensive overview of available spectral library search engines and highlights their distinct features. Additionally, resources providing spectral libraries are summarized and tools presented that extend experimental spectral libraries by simulating spectra. Finally, spectrum clustering algorithms are discussed that utilize the same spectrum-to-spectrum matching algorithms as spectral library search engines and allow novel methods to analyse proteomics data. PMID:26616598

  15. Library service to dental practitioners.

    OpenAIRE

    Ashin, E R

    1983-01-01

    Dental school libraries offer resources of value to dental practitioners, but do not always consider practitioners to be primary clientele. A survey was conducted among the sixty U.S. dental school libraries to examine policies and attitudes toward service to practitioners. Although library use by dentists is estimated to be low, most libraries are willing to serve them as long as it does not reduce the libraries' ability to assist students and faculty. Of the respondents, 57% replied that th...

  16. Share and share alike: encouraging the reuse of academic resources through the Scottish electronic Staff Development Library

    Directory of Open Access Journals (Sweden)

    Lorna M. Campbell

    2001-12-01

    Full Text Available The Scottish electronic Staff Development Library (http://www.sesdl.scotcit.acuk is an ongoing collaborative project involving the Universities of Edinburgh, Paisley and Strathclyde which has been funded by SHEFC as part of their current ScotCIT Programme (http:llwww.scotcit.ac.uk. This project is being developed in response to the increasing demand for flexible, high-quality staff development materials.

  17. The Egyptian Web Logs as an Information Resource with a Special Indication to Library and Librarian's Blogs

    OpenAIRE

    Shayma' Ismael Abbas Ismael

    2007-01-01

    An article is discussing The Egyptian Web Logs ,library blogs and librarian's blogs at the world wide web as one of information electronic sources so this article is answering on some questions. what is conception of blogs?, What is historic development and origin of blogs?, What are reasons to fame and spread blogs between internet employees ?, what is blogging software to create blog?, what is directory and search engine of blogs?, what is characteristics of successful blogs? and concen...

  18. Sex Chromosome Evolution in Amniotes: Applications for Bacterial Artificial Chromosome Libraries

    OpenAIRE

    Janes, Daniel E.; Nicole Valenzuela; Tariq Ezaz; Chris Amemiya; Edwards, Scott V.

    2011-01-01

    Variability among sex chromosome pairs in amniotes denotes a dynamic history. Since amniotes diverged from a common ancestor, their sex chromosome pairs and, more broadly, sex-determining mechanisms have changed reversibly and frequently. These changes have been studied and characterized through the use of many tools and experimental approaches but perhaps most effectively through applications for bacterial artificial chromosome (BAC) libraries. Individual BAC clones carry 100–200 kb of seque...

  19. An overview of the Phalaenopsis orchid genome through BAC end sequence analysis

    Directory of Open Access Journals (Sweden)

    Hsiao Yu-Yun

    2011-01-01

    Full Text Available Abstract Background Phalaenopsis orchids are popular floral crops, and development of new cultivars is economically important to floricultural industries worldwide. Analysis of orchid genes could facilitate orchid improvement. Bacterial artificial chromosome (BAC end sequences (BESs can provide the first glimpses into the sequence composition of a novel genome and can yield molecular markers for use in genetic mapping and breeding. Results We used two BAC libraries (constructed using the BamHI and HindIII restriction enzymes of Phalaenopsis equestris to generate pair-end sequences from 2,920 BAC clones (71.4% and 28.6% from the BamHI and HindIII libraries, respectively, at a success rate of 95.7%. A total of 5,535 BESs were generated, representing 4.5 Mb, or about 0.3% of the Phalaenopsis genome. The trimmed sequences ranged from 123 to 1,397 base pairs (bp in size, with an average edited read length of 821 bp. When these BESs were subjected to sequence homology searches, it was found that 641 (11.6% were predicted to represent protein-encoding regions, whereas 1,272 (23.0% contained repetitive DNA. Most of the repetitive DNA sequences were gypsy- and copia-like retrotransposons (41.9% and 12.8%, respectively, whereas only 10.8% were DNA transposons. Further, 950 potential simple sequence repeats (SSRs were discovered. Dinucleotides were the most abundant repeat motifs; AT/TA dimer repeats were the most frequent SSRs, representing 253 (26.6% of all identified SSRs. Microsynteny analysis revealed that more BESs mapped to the whole-genome sequences of poplar than to those of grape or Arabidopsis, and even fewer mapped to the rice genome. This work will facilitate analysis of the Phalaenopsis genome, and will help clarify similarities and differences in genome composition between orchids and other plant species. Conclusion Using BES analysis, we obtained an overview of the Phalaenopsis genome in terms of gene abundance, the presence of repetitive

  20. As Blood Alcohol Content (BAC) Increases, So Does Impairment | NIH MedlinePlus the Magazine

    Science.gov (United States)

    ... on. Feature: Rethinking Drinking As Blood Alcohol Content (BAC) Increases, So Does Impairment Past Issues / Spring 2014 ... For purposes of law enforcement, blood alcohol content (BAC) is used to define intoxication and provides a ...

  1. Efficient conditional knockout targeting vector construction using co-selection BAC recombineering (CoSBR)

    OpenAIRE

    Robert J. Newman; Roose-Girma, Merone; Warming, Søren

    2015-01-01

    A simple and efficient strategy for Bacterial Artificial Chromosome (BAC) recombineering based on co-selection is described. We show that it is possible to efficiently modify two positions of a BAC simultaneously by co-transformation of a single-stranded DNA oligo and a double-stranded selection cassette. The use of co-selection BAC recombineering reduces the DNA manipulation needed to make a conditional knockout gene targeting vector to only two steps: a single round of BAC modification foll...

  2. Cypriot libraries

    OpenAIRE

    John F. Harvey

    1982-01-01

    Describes the current state of librarianship and bibliography in Cyprus, with separate sections for the Greek and Turkish sectors. Although there is no national library in the Greek sector there are 5 types of public library: Nicosia public library; Limassol, Larnaca and Paphos public libraries; community libraries; mobile libraries; and foreign cultural centre libraries. Schools and colleges in the Greek centre are well provided with libraries and most government departments sponsor special ...

  3. 76 FR 44055 - BAC Home Loans Servicing, LP, et al.; Notice of Application and Temporary Order

    Science.gov (United States)

    2011-07-22

    ... COMMISSION BAC Home Loans Servicing, LP, et al.; Notice of Application and Temporary Order July 18, 2011... Act, with respect to an injunction entered against BAC Home Loans Servicing, LP (``HLS'') on May 31... (``BAC''). HLS is an entity that services mortgage loans and provides mortgage services,...

  4. Academic Libraries in Japan

    Science.gov (United States)

    Cullen, Rowena; Nagata, Haruki

    2008-01-01

    Academic libraries in Japan are well resourced by international standards, and support Japan's internationally recognized research capability well, but there are also ways in which they reflect Japan's strong bureaucratic culture. Recent changes to the status of national university libraries have seen a new interest in customer service, and…

  5. Research on digital teaching resource library mode in distance education based on cloud computing%基于云计算的远程教育数字化教学资源库模式研究

    Institute of Scientific and Technical Information of China (English)

    刘晓丹; 蒋漪涟

    2015-01-01

    In order to realize the sharing of learning resources in the distance education and meet the needs of dif -ferent learners , it analyzes the digital teaching resource library in distance education from the cloud computing technology , proposes the teaching resource library model and the key technology , discusses the construction of teaching resource library mode , establishes the logical structure and platform structure of resource library mode , points out the broad prospect of the application of cloud computing technology in the construction of distance edu -cation resources .%为实现远程教育中的学习资源共享,满足不同学习者对资源的需求,对远程教育数字化教学资源库模式进行了研究. 从云计算技术、教学资源库模式、关键技术3个方面探讨了教学资源库的模式建设,阐述了资源库模式的逻辑架构和平台架构,指出了云计算技术在远程教育资源库建设中应用的广阔前景.

  6. News from the Library

    CERN Multimedia

    CERN Library

    2010-01-01

    The LHC Library to be merged with the Central Library. Not everyone knows that CERN Scientific Information Service currently counts three physical libraries on site. The Central Library is located in Building 52 and there are two satellite libraries located respectively in building 30 (the LHC Library) and in building 864 on Prévessin site (the SPS Library). Moreover, the Legal Service Library is located in Building 60. In the past, there have been at CERN up to 6 satellite libraries; they were essential at a time when information was only in paper form and having multiple copies of documents located in several places at CERN was useful to facilitate scientific research. Today, this need is less critical as most of our resources are online. That is why, following a SIPB (Scientific Information Policy Board) decision, the collections of the LHC Library will be merged this summer with the Central collection. This reorganization and centralization of resources will improve loan services. The SP...

  7. An efficient approach to BAC based assembly of complex genomes

    Czech Academy of Sciences Publication Activity Database

    Visendi, P.; Berkman, P.J.; Hayashi, S.; Golicz, A.A.; Bayer, P.E.; Ruperao, P.; Hurgobin, B.; Montenegro, J.; Chan, C.K.K.; Staňková, Helena; Batley, J.; Šimková, Hana; Doležel, Jaroslav; Edwards, D.

    2016-01-01

    Roč. 12, JAN 20 (2016), s. 2. ISSN 1746-4811 R&D Projects: GA MŠk(CZ) LO1204; GA ČR(CZ) GAP501/12/2554 Institutional support: RVO:61389030 Keywords : Next-generation sequencing * SASSY * BAC Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.102, year: 2014

  8. Thermochemical study of the molecule BaC2

    International Nuclear Information System (INIS)

    The enthalpy of the reaction Ba(g)+2C(graph.) = BaC2(g) has been determined by Knudsen effusion mass spectrometry over a barium alloy with noble metals contained in a graphite cell. The value ΔH/sup circle-open/0 = 252.5 +- 11.5kJ mol-1 has been obtained from second- and third-law evaluations of the equilibrium vapor in the temperature range 1778--2286 K. This reaction enthalpy has been combined with ancillary literature data to derive the following thermodynamic properties in kJ mol-1 of gaseous BaC2: the atomization energy, Δ/sub at/H/sup circle-open/0 , 1180.9 +- 12.2; the standard heat of formation, Δ/sub f/H/sup circle-open/298 , 437.1 +- 14.2; the enthalpy of sublimation of BaC2(s), Δ/sub sub/H/sup circle-open/298 , 511.2 +- 17.1; and the bond dissociation energy, D/sup circle-open/0 (Ba--C2), 581.7 +- 14.8. The results are discussed with regard to their possible significance in reactor technology

  9. Iowa DNR - NRGIS Library

    Data.gov (United States)

    Iowa State University GIS Support and Research Facility — The Natural Resources Geographic Information System (NRGIS) Library is a Geographic Information System (GIS) repository developed and maintained by the GIS Section...

  10. 基于内部云存储的图书馆数据资源存储研究%Design for Library Data Resources Storage System Based on Internal Cloud Storage

    Institute of Scientific and Technical Information of China (English)

    乔杨

    2012-01-01

    Reliable storage environment is the basis for library digital resources providing efficient services for users. Based on the discussion of library digital resources storage different technologies, cloud storage connotation and applications in library are analyzed in this paper. According to the characteristics of library digital resources, a storage architecture based on internal cloud storage for library digital resources is designed. And the hierarchical structure, the methods of access control, expansion and deployment are detailed.%可靠存储环境是图书馆数字资源为用户提供高效服务的保障。在阐述图书馆数据资源存储技术基础上,分析了云存储内涵及在图书馆中的应用,探讨基于内部云存储的图书馆数字资源数据存储方案,详细分析了其层次架构、访问控制、扩展和部署方法。

  11. Co-construction and Sharing of Public Libraries ’ Exhibition Resources---Taking the Wenyuan Exhibition of Shanxi Library as an Example%公共图书馆展览资源的共建共享--以山西省图书馆“文源视界”为例

    Institute of Scientific and Technical Information of China (English)

    李红; 石焕发

    2014-01-01

    以山西省图书馆“文源视界”为例,概述了山西省公共图书馆展览工作现状,探讨了山西省公共图书馆展览资源共建共享的途径。%Taking the Wenyuan Exhibition of Shanxi Library as an example, this paper reviews the current status of the exhibition work of public libraries in Shanxi Province, and probes into the paths for the co-construction and sharing of exhibition resources of public libraries in Shanxi Province.

  12. On the Application of Flexible Management in Human Resource Management of Library%柔性管理在图书馆人力资源管理中的应用研究

    Institute of Scientific and Technical Information of China (English)

    袁峰; 叶丽红

    2012-01-01

    在简述柔性管理涵义的基础上,针对图书馆人力资源管理现状,提出图书馆在人力资源管理中应用柔性管理的策略。%Based on the description of the meaning of flexible management,this paper puts forward the strategies of the application of flexible management in library human resource management according to the current situation of library human resource management.

  13. 论新信息环境下中小型图书馆信息资源建设%Discussion on Information Resources Construction of Small and Medium-sized Libraries in the New Information Environment

    Institute of Scientific and Technical Information of China (English)

    叶菁

    2015-01-01

    This paper discusses the positioning of information resources construction of small and medium-sized libraries in the new information environment, and probes into some effective countermeasures for small and medium-sized libraries to carry out the information resources construction in new information environment.%论述了新信息环境下中小型图书馆信息资源建设的定位,探讨了新信息环境下中小型图书馆信息资源建设的有效对策.

  14. Action and Timing of BacC and BacD in the Late Stages of Biosynthesis of the Dipeptide Antibiotic Bacilysin

    OpenAIRE

    Parker, Jared B.; Walsh, Christopher T.

    2013-01-01

    Biosynthesis of the dipeptide antibiotic bacilysin, encoded by the seven B. subtilis genes bacA-G, involves diversion of flux from prephenate to the noncognate amino acid anticapsin. The anticapsin warhead is then ligated to the C-terminus of l-alanine to produce mature bacilysin. We have previously noted the formation of two diastereomers of tetrahydrotyrosine (4S- and 4R-H4Tyr) by tandem action of the four purified enzymes BacABGF. BacC (oxidase) and BacD (ligase) have been hypothesized to ...

  15. Comparison of the BacT/Alert PF Pediatric FAN Blood Culture Bottle with the Standard Pediatric Blood Culture Bottle, the Pedi-BacT

    OpenAIRE

    Krisher, Karen K.; Gibb, Patrick; Corbett, Sandra; Church, Deidre

    2001-01-01

    The performance of the BacT/Alert PF (Organon-Teknika Corp., Durham, N.C.), a new nonvented pediatric FAN blood culture bottle, was compared to that of the original pediatric bottle, the Pedi-BacT, with matched aerobic cultures obtained from two separate facilities. A total of 244 clinically significant isolates were recovered from 4,015 compliant pairs. Among the positive cultures, 170 (70%) isolates were detected in both the BacT/Alert PF and the Pedi-BacT bottles, while 47 (19%) isolates w...

  16. Novel SSR markers from BAC-end sequences, DArT arrays and a comprehensive genetic map with 1,291 marker loci for chickpea (Cicer arietinum L..

    Directory of Open Access Journals (Sweden)

    Mahendar Thudi

    Full Text Available Chickpea (Cicer arietinum L. is the third most important cool season food legume, cultivated in arid and semi-arid regions of the world. The goal of this study was to develop novel molecular markers such as microsatellite or simple sequence repeat (SSR markers from bacterial artificial chromosome (BAC-end sequences (BESs and diversity arrays technology (DArT markers, and to construct a high-density genetic map based on recombinant inbred line (RIL population ICC 4958 (C. arietinum×PI 489777 (C. reticulatum. A BAC-library comprising 55,680 clones was constructed and 46,270 BESs were generated. Mining of these BESs provided 6,845 SSRs, and primer pairs were designed for 1,344 SSRs. In parallel, DArT arrays with ca. 15,000 clones were developed, and 5,397 clones were found polymorphic among 94 genotypes tested. Screening of newly developed BES-SSR markers and DArT arrays on the parental genotypes of the RIL mapping population showed polymorphism with 253 BES-SSR markers and 675 DArT markers. Segregation data obtained for these polymorphic markers and 494 markers data compiled from published reports or collaborators were used for constructing the genetic map. As a result, a comprehensive genetic map comprising 1,291 markers on eight linkage groups (LGs spanning a total of 845.56 cM distance was developed (http://cmap.icrisat.ac.in/cmap/sm/cp/thudi/. The number of markers per linkage group ranged from 68 (LG 8 to 218 (LG 3 with an average inter-marker distance of 0.65 cM. While the developed resource of molecular markers will be useful for genetic diversity, genetic mapping and molecular breeding applications, the comprehensive genetic map with integrated BES-SSR markers will facilitate its anchoring to the physical map (under construction to accelerate map-based cloning of genes in chickpea and comparative genome evolution studies in legumes.

  17. Services and Resources to Engineers: A Case Study of Outreach and Marketing, Assessment, and Future Directions in a Research Library

    Science.gov (United States)

    Torrence, Matt

    2015-01-01

    The literature, activities, and resource needs of engineering students and faculty provide insight into a demographic that is often among the early-adopters of new technologies, tools, and methods of sharing information. Despite the often non-bibliographic nature of their research efforts, there are numerous elements of the traditional service…

  18. Sorghum Mutant Library as a Genetic Resource to Improve Biomass Yield and BioEnergy Conversion Efficiency

    Science.gov (United States)

    Due to the dwindling of mineral energy resources and soaring fuel price, agriculture sector is called to provide bioenergy in addition to the traditional role of providing humanity with food, fiber, and feed. Freshwater is likely a major challenge for developing biofuels because the trend of global ...

  19. Digital Libraries on the Internet.

    Science.gov (United States)

    Sharon, Taly; Frank, Ariel J.

    This paper discusses digital libraries on the Internet. The resource repository hierarchy, consisting of two major paradigms, search engines (SEs) and digital libraries, is presented. SEs are classified into three categories: basic-SE, directory, and meta-SE. The following six major characteristics of a library are summarized: collection of data…

  20. The Library and the Bazaar: Open Content and Libraries

    OpenAIRE

    Hauptman, Greer L.

    2008-01-01

    This essay will consider new copyright models in libraries, and how libraries can and should modify their own systems to promote and provide access to open content. It focuses on the reasoning behind supporting new models and methods of distribution, especially with regards to open licenses like Creative Commons, and the resources and systems libraries have developed to provide access to open licensed work. The paper examines the current roles libraries take in promoting Creative Commons and ...

  1. Sequence composition of BAC clones and SSR markers mapped to Upland cotton chromosomes 11 and 21 targeting resistance to soil-borne pathogens

    Science.gov (United States)

    Wang, Congli; Ulloa, Mauricio; Shi, Xinyi; Yuan, Xiaohui; Saski, Christopher; Yu, John Z.; Roberts, Philip A.

    2015-01-01

    Genetic and physical framework mapping in cotton (Gossypium spp.) were used to discover putative gene sequences involved in resistance to common soil-borne pathogens. Chromosome (Chr) 11 and its homoeologous Chr 21 of Upland cotton (G. hirsutum) are foci for discovery of resistance (R) or pathogen-induced R (PR) genes underlying QTLs involved in response to root-knot nematode (Meloidogyne incognita), reniform nematode (Rotylenchulus reniformis), Fusarium wilt (Fusarium oxysporum f.sp. vasinfectum), Verticillium wilt (Verticillium dahliae), and black root rot (Thielaviopsis basicola). Simple sequence repeat (SSR) markers and bacterial artificial chromosome (BAC) clones from a BAC library developed from the Upland cotton Acala Maxxa were mapped on Chr 11 and Chr 21. DNA sequence through Gene Ontology (GO) of 99 of 256 Chr 11 and 109 of 239 Chr 21 previously mapped SSRs revealed response elements to internal and external stimulus, stress, signaling process, and cell death. The reconciliation between genetic and physical mapping of gene annotations from new DNA sequences of 20 BAC clones revealed 467 (Chr 11) and 285 (Chr 21) G. hirsutum putative coding sequences, plus 146 (Chr 11) and 98 (Chr 21) predicted genes. GO functional profiling of Unigenes uncovered genes involved in different metabolic functions and stress response elements (SRE). Our results revealed that Chrs 11 and 21 harbor resistance gene rich genomic regions. Sequence comparisons with the ancestral diploid D5 (G. raimondii), A2 (G. arboreum) and domesticated tetraploid TM-1 AD1 (G. hirsutum) genomes revealed abundance of transposable elements and confirmed the richness of resistance gene motifs in these chromosomes. The sequence information of SSR markers and BAC clones and the genetic mapping of BAC clones provide enhanced genetic and physical frameworks of resistance gene-rich regions of the cotton genome, thereby aiding discovery of R and PR genes and breeding for resistance to cotton diseases. PMID

  2. The Construction of Library Digital Resources under the Background of the Development of Public Digital Culture%公共数字文化发展背景下的图书馆数字资源建设

    Institute of Scientific and Technical Information of China (English)

    许建业

    2015-01-01

    论文概述了公共数字文化发展背景,探讨了图书馆在公共数字文化建设中的定位与任务,以江苏全省公共图书馆数字化建设和南京图书馆数字资源建设实践为例,围绕图书馆数字资源建设主要内容,提出了相关建议和措施。%This paper outlines the background of public digital culture development, and discusses the positioning and task of library in the construction of public digital culture. Taking the digital construction of public libraries in Jiangsu Province and the concrete practice of Nanjing Library as examples, it puts forward the relevant suggestions and measures on library digital resources construction.

  3. Evidence Based Research Activities, Interests and Opportunities Exist for Practitioners in all Library Sectors in the British Isles. A review of: McNicol, Sarah. “Is Research an Untapped Resource in the Library and Information Profession?” Journal of Librarianship and Information Science 36.3 (September 2004:119-26.

    Directory of Open Access Journals (Sweden)

    Julie McKenna

    2006-03-01

    technology (ICT was an area of planned future research in all libraries, as were user needs and user behaviour. The most frequently cited barriers to research activity across all sectors were lack of time and financial resources. Staff skills and the lack of focus on practical problems to solve were indicated as a barrier in health, public and academic libraries. Libraries reported a range of common uses for the research findings including: informing strategic and service planning; providing benchmarking data and measuring the effectiveness of services; identification of marketing and public relations opportunities; discovery of staff training needs; and use of the results to demonstrate the value of libraries to funding bodies. Conclusion – This study provides insight into practitioner‐focused areas of research interest and possible areas for future investigation. As the author reports in her conclusion, the survey results cannot be considered representative of the wider population. Since research interests often overlap, a sector wide or cross‐sectoral research approach should be considered to allow library staff to identify and resolve common problems. Wide dissemination of research results within the practitioner community would be of benefit to all. Greater communication between practitioner and information science communities is also encouraged, as these communities’ work is mutually beneficial.

  4. 图书馆光盘型电子资源编目方法探析%Aanlysis of the Cataloging Method for CD-ROM Resources in Library

    Institute of Scientific and Technical Information of China (English)

    王晶

    2011-01-01

    This paper defines the conception of CD-ROM resources,and analyses the cataloging for CD-ROM resources in library with the examples of cataloging work.%对光盘型电子资源进行了界定,结合工作实例对图书馆光盘型电子资源的编目进行分析。

  5. Construction and Characterization of Three Wheat Bacterial Artificial Chromosome Libraries

    Directory of Open Access Journals (Sweden)

    Wenjin Cao

    2014-11-01

    Full Text Available We have constructed three bacterial artificial chromosome (BAC libraries of wheat cultivar Triticum aestivum Wangshuibai, germplasms T. monococcum TA2026 and TA2033. A total of 1,233,792,170,880 and 263,040 clones were picked and arrayed in 384-well plates. On the basis of genome sizes of 16.8 Gb for hexaploid wheat and 5.6 Gb for diploid wheat, the three libraries represented 9.05-, 2.60-, and 3.71-fold coverage of the haploid genomes, respectively. An improved descending pooling system for BAC libraries screening was established. This improved strategy can save 80% of the time and 68% of polymerase chain reaction (PCR with the same successful rate as the universal 6D pooling strategy.

  6. Standards for hospital libraries 2002

    OpenAIRE

    Gluck, Jeannine Cyr; Hassig, Robin Ackley; Balogh, Leeni; Bandy, Margaret; Doyle, Jacqueline Donaldson; Kronenfeld, Michael R.; Lindner, Katherine Lois; Murray, Kathleen; Petersen, JoAn; Rand, Debra C.

    2002-01-01

    The Medical Library Association's “Standards for Hospital Libraries 2002” have been developed as a guide for hospital administrators, librarians, and accrediting bodies to ensure that hospitals have the resources and services to effectively meet their needs for knowledge-based information. Specific requirements for knowledge-based information include that the library be a separate department with its own budget. Knowledge-based information in the library should be directed by a qualified libr...

  7. Strategic marketing planning in library

    OpenAIRE

    Karmen Štular-Sotošek

    2000-01-01

    The article is based on the idea that every library can design instruments for creating events and managing the important resources of today's world, especially to manage the changes. This process can only be successful if libraries use adequate marketing methods. Strategic marketing planning starts with the analysis of library's mission, its objectives, goals and corporate culture. By analysing the public environment, the competitive environment and the macro environment, libraries recognise...

  8. Mobile technologies for every library

    CERN Document Server

    Gleason, Ann Whitney

    2015-01-01

    If you are wondering what mobile technology adoption means for your library or how to get started, Mobile Technologies for Every Library will answer your questions! This book will detail the opportunities and pitfalls in using mobile technology in libraries. It will also address platforms, options, security, and best practices for starting new mobile services programs or improving existing services. Gleason previews many useful apps for libraries. Web links and resources are also included.

  9. Genomic Resources for Water Yam (Dioscorea alata L.): Analyses of EST-Sequences, De Novo Sequencing and GBS Libraries

    Science.gov (United States)

    Saski, Christopher A.; Bhattacharjee, Ranjana; Scheffler, Brian E.; Asiedu, Robert

    2015-01-01

    The reducing cost and rapid progress in next-generation sequencing techniques coupled with high performance computational approaches have resulted in large-scale discovery of advanced genomic resources in several model and non-model plant species. Yam (Dioscorea spp.) is a major food and cash crop in many countries but research efforts have been limited to understand the genetics and generate genomic information for the crop. The availability of a large number of genomic resources including genome-wide molecular markers will accelerate the breeding efforts and application of genomic selection in yams. In the present study, several methods including expressed sequence tags (EST)-sequencing, de novo sequencing, and genotyping-by-sequencing (GBS) profiles on two yam (Dioscorea alata L.) genotypes (TDa 95/00328 and TDa 95-310) was performed to generate genomic resources for use in its improvement programs. This includes a comprehensive set of EST-SSRs, genomic SSRs, whole genome SNPs, and reduced representation SNPs. A total of 1,152 EST-SSRs were developed from >40,000 EST-sequences generated from the two genotypes. A set of 388 EST-SSRs were validated as polymorphic showing a polymorphism rate of 34% when tested on two diverse parents targeted for anthracnose disease. In addition, approximately 40X de novo whole genome sequence coverage was generated for each of the two genotypes, and a total of 18,584 and 15,952 genomic SSRs were identified for TDa 95/00328 and TDa 95-310, respectively. A custom made pipeline resulted in the selection of 573 genomic SSRs common across the two genotypes, of which only eight failed, 478 being polymorphic and 62 monomorphic indicating a polymorphic rate of 83.5%. Additionally, 288,505 high quality SNPs were also identified between these two genotypes. Genotyping by sequencing reads on these two genotypes also revealed 36,790 overlapping SNP positions that are distributed throughout the genome. Our efforts in using different approaches

  10. Genomic Resources for Water Yam (Dioscorea alata L.: Analyses of EST-Sequences, De Novo Sequencing and GBS Libraries.

    Directory of Open Access Journals (Sweden)

    Christopher A Saski

    Full Text Available The reducing cost and rapid progress in next-generation sequencing techniques coupled with high performance computational approaches have resulted in large-scale discovery of advanced genomic resources in several model and non-model plant species. Yam (Dioscorea spp. is a major food and cash crop in many countries but research efforts have been limited to understand the genetics and generate genomic information for the crop. The availability of a large number of genomic resources including genome-wide molecular markers will accelerate the breeding efforts and application of genomic selection in yams. In the present study, several methods including expressed sequence tags (EST-sequencing, de novo sequencing, and genotyping-by-sequencing (GBS profiles on two yam (Dioscorea alata L. genotypes (TDa 95/00328 and TDa 95-310 was performed to generate genomic resources for use in its improvement programs. This includes a comprehensive set of EST-SSRs, genomic SSRs, whole genome SNPs, and reduced representation SNPs. A total of 1,152 EST-SSRs were developed from >40,000 EST-sequences generated from the two genotypes. A set of 388 EST-SSRs were validated as polymorphic showing a polymorphism rate of 34% when tested on two diverse parents targeted for anthracnose disease. In addition, approximately 40X de novo whole genome sequence coverage was generated for each of the two genotypes, and a total of 18,584 and 15,952 genomic SSRs were identified for TDa 95/00328 and TDa 95-310, respectively. A custom made pipeline resulted in the selection of 573 genomic SSRs common across the two genotypes, of which only eight failed, 478 being polymorphic and 62 monomorphic indicating a polymorphic rate of 83.5%. Additionally, 288,505 high quality SNPs were also identified between these two genotypes. Genotyping by sequencing reads on these two genotypes also revealed 36,790 overlapping SNP positions that are distributed throughout the genome. Our efforts in using

  11. Cloud Computing and Your Library

    Science.gov (United States)

    Mitchell, Erik T.

    2010-01-01

    One of the first big shifts in how libraries manage resources was the move from print-journal purchasing models to database-subscription and electronic-journal purchasing models. Libraries found that this transition helped them scale their resources and provide better service just by thinking a bit differently about their services. Likewise,…

  12. BacMet: antibacterial biocide and metal resistance genes database

    OpenAIRE

    Pal, Chandan; Bengtsson-Palme, Johan; Rensing, Christopher; Kristiansson, Erik; Larsson, D.G. Joakim

    2013-01-01

    Antibiotic resistance has become a major human health concern due to widespread use, misuse and overuse of antibiotics. In addition to antibiotics, antibacterial biocides and metals can contribute to the development and maintenance of antibiotic resistance in bacterial communities through co-selection. Information on metal and biocide resistance genes, including their sequences and molecular functions, is, however, scattered. Here, we introduce BacMet (http://bacmet.biomedicine.gu.se)—a manua...

  13. Effect of DNA damaging agents on Bac. stearothermophilus

    International Nuclear Information System (INIS)

    A thermophilic micro-organism Bac. stearothermophilus showes a high resistance to the effect of such DNA damaging agents as NMM, UV- and γ-radiation. Due to adaptation to high temperatures, at which intensive depurinization and depyrimidinization of DNA take place, thermophilic micro-organisms are suggested to acquire evolutionary a powerful system of repair of DNA damages, particularly, of apurine and apyrimidine sites

  14. Construction and characterization of a bacterial artificial chromosome library of thermo-sensitive genic male-sterile rice 5460S

    Institute of Scientific and Technical Information of China (English)

    邱芳; 金德敏; 伏健民; 张超良; 谢纬武; 王斌; 杨仁崔; 张洪斌

    1999-01-01

    In order to develop a detailed physical map of the thermo-sensitive genie male-sterile (TGMS) gene-encompassing region and finally clone the TGMS gene, a high-quality rice bacterial artificial chromosome (BAC) library from TGMS rice 5460S was constructed. The method of constructing BAC library was examined and optimized. The 5460S library consists of 19 584 BAC clones with an average insert size of 110 kb, which represents about 5 times rice haploid genome equivalents. Rice inserts of up to 140 kb and 250 kb were isolated and appeared stable after 100 generations of serial growth. Hybridization of BAC clones with mitochondrial and chloroplastic genes as probes demonstrated that this library has no organellar contamination. The 5460S library was screened with 3 molecular markers linked to tmsl gene as probes and at least 1 BAC clone was identified with each probe. The insert ends of positive clones were successfully isolated using thermal asymmetric interlaced PCR (TAIL-PCR) technique.

  15. International librarianship. An electronic resources pathfinder. A term paper for the References Services course at the Master in Library Science Program, Department of Library and Information Studies, State University of New York at Buffalo, Buffalo, NY (USA)

    OpenAIRE

    Muela-Meza, Zapopan Martín

    2001-01-01

    This library guide or pathfinder is made as a term paper for the References Services course at the Master in Library Science Program at the State University of New York at Buffalo, Buffalo, NY, USA; Associate Professor of the course Dr. Lorna Peterson, April 23, 2001. It lists sources in electronic format to introduce undergraduate and graduate students of the State University of New York at Buffalo to the research and study of Library and Information Science with an international scope. Cons...

  16. A BAC clone of MDV strain GX0101 with REV-LTR integration retained its pathogenicity

    Institute of Scientific and Technical Information of China (English)

    SUN AiJun; LAWRENCE Petherbridge; ZHAO YuGuang; LI YanPeng; NAIR Venugopal K; CUI ZhiZhong

    2009-01-01

    The complete genome of Marek's disease virus (MDV) strain GX0101,which was integrated with the LTR sequences of REV,was cloned in Escherichia coli as a bacterial artificial chromosome (BAC).BAC vector sequences were introduced into the US2 locus of the MDV genome by homologous recombination.The viral DNA containing the BAC vector was used to transform Escherichia coli strain of DH10B.Then the recombinant virus was successfully rescued by transfection of the recombinant BAC DNA into primary chicken embryo fibroblast (CEF).This BAC viral clone was named bac-GX0101.When the reconstituted virus was inoculated into 1-day-old birds,visceral tumors could be detected as early as 62 d post infection.There was no difference in growth ability and pathogenicity to birds between the BAC derived virus and its parental virus.The BAC derived virus maintained its oncogenicity and immunosuppressive effects.In conclusion,the complete genome of GX0101 strain was successfully cloned into BAC and the infectious clone was rescued.With the powerful BAC manipulation system,the infectious clone will provide a useful tool for further understanding the functional roles of the inserted REV-LTR sequence in the GX0101 strain of MDV.

  17. Experimental study of 188Re-BAC5 radioimmunotherapy on nasopharyngeal carcinoma

    International Nuclear Information System (INIS)

    The feasibility of radioimmunotherapy for NPC with 188Re-BAC5, a kind of monoclonal antibody to NPC, was investigated. The 188Re-BAC5 was prepared by direct labeling method and its biological activity was determined. The MTT method was used to determine the inhibition effect of the 188Re-BAC5 on CNE-2 cell line culture, and the destruction effect on CNE-2 multicellular spheroids model was also observed. The control group were 188Re-BSA and 188ReO4-. The biological activity of BAC5 was 1:780000 after purification. The labeling yield of 188Re-BAC5 was above 80%. The immuno-activity of 188Re-BAC5 was 61% ± 15%. The results of MTT showed that the inhibition effect of 188Re-BAC5 group was much stronger than the control group (P188Re-BAC5 had a obvious destruction effect on spheroids, and there was a significant difference between 188Re-BAC5 and the control group. In conclusion, 188Re-BAC5 may serve a possible way in the treatment of NPC in the near future

  18. On the Construction of Subject Resources and Subject Service in American Academic Libraries:Taking Four Business Libraries in America as Examples%美国高校图书馆学科资源建设及学科服务研究——以四所美国商学院图书馆为例

    Institute of Scientific and Technical Information of China (English)

    蔡金燕

    2015-01-01

    American academic libraries are experienced in the construction of subject resources and subject service, especially in the business libraries. Various categories of resources, like physical resources, databases, kinds of guides, course resources, self-built databases, are abundant in quantity in these business libraries. They provide subject services focusing on courses, researches and careers. Chinese academic libraries should learn from them.%美国高校图书馆学科资源建设和学科服务实践成熟、经验丰富,尤其是商学院图书馆,实体资源、电子数据库、各种资源导航、课程资源、自建数据库等资源丰富,实现以课程为中心、以研究为中心、以职业为中心三种方式进行学科服务,值得国内高校图书馆借鉴.

  19. Books, children, dogs, artists: library programs for the entire family.

    Science.gov (United States)

    Haver, Mary Katherine

    2014-01-01

    The promotion of library resources and services is a continuous process for all libraries, especially hospital family resource center libraries. Like public libraries, a family resource center can utilize programs as a pathway for connecting with and developing awareness of library resources and services available to patient families. This column describes the programs currently offered for All Children's Hospital Johns Hopkins Medicine patient families, marketing initiatives to promote these programs, and utilization of grant funding to supplement a program. PMID:24735270

  20. 依托文化资源共享工程拓展县图书馆延伸服务%Relying on Cultural Resources Sharing Project, Developing the County Library Extension Services

    Institute of Scientific and Technical Information of China (English)

    曹志萍

    2012-01-01

    This paper analyzes the coexistence reality of opportunities and challenges of the county libraries under the network environment, proposes the county library must change the service concept, improve the construction of the library. By relying on the excellent resources of the National Cultural Resources Sharing Project, county library will expand the county level library extension services through service to the county ,community and rural areas so that the cultural information will spread to the county wide and provide full service to people of the county.%本文分析了网络环境下县级图书馆机遇与挑战并存的现实,提出县级图书馆必须转变服务理念,搞好图书馆阵地建设,并依托全国文化资源共享工程优秀资源,拓展县级图书馆延伸服务,通过服务县城、服务社区、服务农村,使文化信息真正普及到全县范围,全方位服务全县人民。

  1. Library Employment Sources on the Internet

    Science.gov (United States)

    Barr, Catherine

    2012-01-01

    This article presents a list of online resources for library job seekers. This includes general sites/portals like American Library Association (ALA): Education & Careers and Canadian Library Association: Library Careers. It also includes sites by sector, employment agencies/commercial services, listservs and networking sites.

  2. The Faced Challenge and Innovation Development of Military Academy Library in Resources Co-construction & Sharing%军校图书馆资源共建共享面临的挑战与创新发展

    Institute of Scientific and Technical Information of China (English)

    于厚海; 赵正国

    2015-01-01

    新时期军校图书馆资源共建共享面临着严峻挑战和发展机遇。当前制约图书馆资源共建共享的主要因素包括主观和客观两个方面。必须从理念、机制和制度等方面入手,推进军校图书馆资源共建共享体系创新发展。%The resources co -construction and sharing of military academy library face new challenges and opportunities in the new era. Nowadays, the factors restraining the resources co-construction and sharing include in both subjective and objective aspects. It is necessary to promote the innovative development system for resources co-construction and sharing of military academy library from the aspects of concept, mechanism, and institution, etc.

  3. Construction and characterization of a bacterial artificial chromosome library of the maize inbred line Qi319

    Directory of Open Access Journals (Sweden)

    Chun Hua Mu

    2016-03-01

    Full Text Available Zea mays L. has been the most cultivated crop and the crop with the largest yield in China since 2012. We constructed a bacterial artificial chromosome (BAC library for the maize inbred line Qi319, which may be used as a key source for disease-resistant maize breeding in China. The BAC contains 270,720 clones, with an average insert size of 90 kb. The coverage of the library is about 10.43 genome equivalents when considering a haploid genome size of 2300 Mb, providing a 99.99% likelihood of isolating any maize gene or sequence in the library. An average of 12 clones were obtained by polymerase chain reaction screening by using primer pairs linked to the genes for resistance to maize southern rust and rough dwarf. The results indicate that the library can satisfy the requirements for recovering specific sequences. The library is available to researchers to whom it may be of interest.

  4. Resources.

    Science.gov (United States)

    Stewart, John; MacDonald, Ian

    1980-01-01

    Presents a guide to resources on television drama available to teachers for classroom use in television curriculum. Lists American and British television drama videorecordings of both series and individual presentations and offers a bibliography of "one-off" single fiction plays produced for British television. (JMF)

  5. Sequenced BAC anchored reference genetic map that reconciles the ten individual chromosomes of Brassica rapa

    OpenAIRE

    Park Beom-Seok; Jin Mina; Van Nguyen Dan; Hossain Md; Lee Seo; Hong Chang; Bae Jina; Choi Su; Kim HyeRan; Bang Jea-Wook; Bancroft Ian; Lim Yong

    2009-01-01

    Abstract Background In view of the immense value of Brassica rapa in the fields of agriculture and molecular biology, the multinational Brassica rapa Genome Sequencing Project (BrGSP) was launched in 2003 by five countries. The developing BrGSP has valuable resources for the community, including a reference genetic map and seed BAC sequences. Although the initial B. rapa linkage map served as a reference for the BrGSP, there was ambiguity in reconciling the linkage groups with the ten chromos...

  6. Library Automation

    OpenAIRE

    Dhakne, B. N.; Giri, V. V; Waghmode, S. S.

    2010-01-01

    New technologies library provides several new materials, media and mode of storing and communicating the information. Library Automation reduces the drudgery of repeated manual efforts in library routine. By use of library automation collection, Storage, Administration, Processing, Preservation and communication etc.

  7. Performance of BAC process for treatment of micro-polluted water

    Institute of Scientific and Technical Information of China (English)

    WANG Chen; MA Fang; SHAN Dan; YANG Ji-xian; Chang Chein-chi

    2008-01-01

    Biological activated carbon (BAC) has been developed on the granular activated carbon by immobi-lization of selected and acclimated species of bacteria to treat the micro-polluted water. The BAC removal effi-ciencies for nitrobenzene, permanganate index, turbidity and ammonia were investigated. Effects of shock load-ing and SEM (Scanning Electron Microscope) observation on BAC were studied. Baekwashing and its intensity of BAC were also discussed. The results showed that BAC took short time to start up and recover to the normal condition after shock loading. The shock loading studies showed that the removal efficiency of BAC was not completely inhibited even at high concentration of nitrobenzene. Backwashing performed once every 10- 20 d,or an average of 15 d. Backwashing intensity was 12-14 L/( s·m2) with air and 3-4 L/( s·m2) with water.

  8. piggyBac can bypass DNA synthesis during cut and paste transposition

    OpenAIRE

    Mitra, Rupak; Fain-Thornton, Jennifer; Craig, Nancy L.

    2008-01-01

    DNA synthesis is considered a defining feature in the movement of transposable elements. In determining the mechanism of piggyBac transposition, an insect transposon that is being increasingly used for genome manipulation in a variety of systems including mammalian cells, we have found that DNA synthesis can be avoided during piggyBac transposition, both at the donor site following transposon excision and at the insertion site following transposon integration. We demonstrate that piggyBac tra...

  9. Library Consortia in Croatia

    OpenAIRE

    2001-01-01

    The problem of the increasing number of information sources and their costs as well as more demanding users on the one side, and limited financial resources on the other, has not avoided Croatia. One of the attempts to deal with this state of affairs is associating libraries into consortia. A consortium could, through cooperative cataloguing, help optimise the processing of library material, it could help in solving the problem of rational ways of continuous education of staff and users and c...

  10. Information Activities in Medical Library : Tokyo Women's Medical College Library

    Science.gov (United States)

    Nishioka, Masayuki

    The library facilities, resource materials, training of librarians and so on are described at first. The library collection is that of middle sized medical library. However, since the facilities are not enough to handle it, it is necessary for the library to be supplemented by information services. Then primary information services such as reading of materials, interlibrary loan and journal acquisition system of the recent issues for each laboratory is outlined. Secondary information services centered around on-line information retrieval service, contents sheet service and preparation of index cards are also described. What a medical library should be is considered in terms of its relation to information services.

  11. 提高大学图书馆数字资源的利用率——以山东农业大学图书馆为例%Improving Digital Resource Utilization Rate in University Libraries ---Taking the Library of Shandong Agricultural University as an example

    Institute of Scientific and Technical Information of China (English)

    何凤丽

    2012-01-01

    Social responsibility of library lies in providing excellent service to the readers. Development and utilization of digi- tal resource in the university library is one important component in today' s library routine work. We take the li- brary in Shandong Agricultural University as an example to investigate and analysis the digital resource situation in utilization by a questionnaire. There existed low utilizations on most digital resource, great utilization differences a- mong data bases and huge anticipations on digital resources services from students. Finally, some advices on digital resource development, utilization and information education were proposed in the expectation to be beneficial for digital resource utilization in university libraries%图书馆的社会责任体现在给读者提供优质的服务。数字资源的建设和利用时当前图书馆重要组成部分,我们以山东农业大学图书馆为例,调查与分析大学生对该馆拥有的数字资源的使用情况,发现目前图书馆普遍存在馆藏数字资源的利用率相对较低、数据库使用差异较大和大学生对数字资源和服务的建设有较高要求等,在资源建设、使用和教育方面提出了建议和措施。

  12. Widespread distribution of the piggyBac transposon in various bactrocera species

    International Nuclear Information System (INIS)

    Full text: The piggyBac transposable element from the Lepidopteran species Trichoplusia ni is currently the most widely used vector for insect transgenesis. Consequently, the presence of piggyBac-like sequences has been investigated, by PCR and Southern analysis, in different species of target genera such as Ceratitis, Bactrocera and Anastrepha, along with Tirhitromina and Rhagoletis. PiggyBac-like sequences were detected in several Bactrocera species. The evolution of the piggyBac-like sequences is discussed with respect to the phylogenies of the hosts. (author)

  13. Ammonia, nitrite and nitrate nitrogen removal from polluted source water with ozonation and BAC processes

    International Nuclear Information System (INIS)

    Studies on the removal of ammonia-, nitrite-, and nitrate nitrogen with ozonation (O3), sand filtration (SF), biological activated carbon (BAC), SF-BAC, and/or O3-BAC processes were carried out in two pilot plants and a full scale plant, respectively. The results showed that all of the tested processes exhibited certain nitrogen removal efficiencies, of which both the O3-SF-BAC and O3-BAC processes were most effective and efficient in removing ammonia nitrogen, with mean removal efficiencies of some 90 and 80 percent, respectively. Ozonation was found able to oxidize some organic nitrogen into ammonia, and nitrite ion into nitrate ion. It was also found out, with interest, that the O3-BAC process can carry the nitrification process to the end under sufficient DO content, as well as more hydrocarbon substrates through ozonation that are more easily assimilated by some strains of nitrobacter that can multiply heterotrophically in its carbon beds. In the BAC process, both the DO and easily assimilated substrate contents were too low in its carbon beds due to no ozonation to sustain nitrobacter growth; but the nitrite conversion bacteria, like nitrosomas, can survive under such conditions. As a result, nitrite or nitrate ion content increased multiply in the effluents from BAC or O3-BAC processes over their influents, respectively. The removal mechanisms of various processes for the three forms of nitrogen were studied and discussed, and the optimum design parameters were determined as well

  14. CRNL library serials list

    International Nuclear Information System (INIS)

    A list of 1900 serial publications (periodicals, society transactions and proceedings, annuals and directories, indexes, newspapers, etc.) is presented with volumes and years held by the Main Library. This library is the largest in AECL as well as one of the largest scientific and technical libraries in North America, and functions as a Canadian resource for nuclear information. A main alphabetical list is followed by broad subject field lists representing research interests, and lists of abstract and index serials, general bibliographic serials, conference indexes, press releases, English translations, and original language journals

  15. 省级公共图书馆文献资源政府采购探析%An Inquiry of Government Procurement of Literature Resources in Provincial Public Library

    Institute of Scientific and Technical Information of China (English)

    赵迎春

    2011-01-01

    文献资源的政府采购,是省级公共图书馆文献资源采购的发展方向。作者通过电话调研的方式,了解了港、澳、台之外的全国32个省级公共图书馆的文献资源采购情况,并对省级公共图书馆文献资源政府采购进行了分析。%The government procurement of literature resources is an important direction of development on literature resources procurement in provincial public library. To know more, the author makes an inquiry on the purchase of literature resources among 32 national provincial public libraries besides Hong Kong, Macau and Taiwan by telephone inquiry. This article is an analysis after investigating on government procurement of literature resources in provincial public libraries.

  16. Sex Chromosome Evolution in Amniotes: Applications for Bacterial Artificial Chromosome Libraries

    Science.gov (United States)

    Janes, Daniel E.; Valenzuela, Nicole; Ezaz, Tariq; Amemiya, Chris; Edwards, Scott V.

    2011-01-01

    Variability among sex chromosome pairs in amniotes denotes a dynamic history. Since amniotes diverged from a common ancestor, their sex chromosome pairs and, more broadly, sex-determining mechanisms have changed reversibly and frequently. These changes have been studied and characterized through the use of many tools and experimental approaches but perhaps most effectively through applications for bacterial artificial chromosome (BAC) libraries. Individual BAC clones carry 100–200 kb of sequence from one individual of a target species that can be isolated by screening, mapped onto karyotypes, and sequenced. With these techniques, researchers have identified differences and similarities in sex chromosome content and organization across amniotes and have addressed hypotheses regarding the frequency and direction of past changes. Here, we review studies of sex chromosome evolution in amniotes and the ways in which the field of research has been affected by the advent of BAC libraries. PMID:20981143

  17. library/mobile: Tips on Designing and Developing Mobile Web Sites

    OpenAIRE

    Hannah Gascho Rempel; Laurie M. Bridges; Kim Griggs

    2009-01-01

    Mobile applications can support learning by making library resources more ubiquitous, by bringing new users to the library through increased accessibility to the resources libraries offer, and by creating a new way to enhance connections between patrons and libraries. This increased use of mobile phones provides an untapped resource for delivering library resources to patrons. The mobile Web is the next step for libraries in providing universal access to resources and information. This articl...

  18. Methylation-sensitive linking libraries enhance gene-enriched sequencing of complex genomes and map DNA methylation domains

    Directory of Open Access Journals (Sweden)

    Bharti Arvind K

    2008-12-01

    Full Text Available Abstract Background Many plant genomes are resistant to whole-genome assembly due to an abundance of repetitive sequence, leading to the development of gene-rich sequencing techniques. Two such techniques are hypomethylated partial restriction (HMPR and methylation spanning linker libraries (MSLL. These libraries differ from other gene-rich datasets in having larger insert sizes, and the MSLL clones are designed to provide reads localized to "epigenetic boundaries" where methylation begins or ends. Results A large-scale study in maize generated 40,299 HMPR sequences and 80,723 MSLL sequences, including MSLL clones exceeding 100 kb. The paired end reads of MSLL and HMPR clones were shown to be effective in linking existing gene-rich sequences into scaffolds. In addition, it was shown that the MSLL clones can be used for anchoring these scaffolds to a BAC-based physical map. The MSLL end reads effectively identified epigenetic boundaries, as indicated by their preferential alignment to regions upstream and downstream from annotated genes. The ability to precisely map long stretches of fully methylated DNA sequence is a unique outcome of MSLL analysis, and was also shown to provide evidence for errors in gene identification. MSLL clones were observed to be significantly more repeat-rich in their interiors than in their end reads, confirming the correlation between methylation and retroelement content. Both MSLL and HMPR reads were found to be substantially gene-enriched, with the SalI MSLL libraries being the most highly enriched (31% align to an EST contig, while the HMPR clones exhibited exceptional depletion of repetitive DNA (to ~11%. These two techniques were compared with other gene-enrichment methods, and shown to be complementary. Conclusion MSLL technology provides an unparalleled approach for mapping the epigenetic status of repetitive blocks and for identifying sequences mis-identified as genes. Although the types and natures of

  19. 基于SWOT分析的高校图书馆数字资源云存储模式探讨%The SWOT Analysis of the Digital Resources of University Library Based on Cloud Storage

    Institute of Scientific and Technical Information of China (English)

    彭文

    2014-01-01

    First elaborated the current situation of library digital resources storage, problems and future resource storage requirements, on this basis, using swot analysis of cloud storage brings to the University Library opportunities and threats, as well as the application of cloud storage library's own advantages and disadvantages, and finally the formation of swot analysis matrix, and puts forward four kinds of strategies of University Library Application cloud development of storage.%本文首先阐述了目前图书馆数字资源存储现状、面临的问题和未来资源存储需求,在此基础上,采用SWOT方法分析云存储给高校图书馆带来的机遇和威胁,以及图书馆自身应用云存储的优势和劣势,最后形成SWOT分析矩阵,并提出高校图书馆应用云存储发展的四种战略对策。

  20. 国内高校图书馆资源共享平台建设探析%On the Construction of Resource Sharing Platform in University Libraries in China

    Institute of Scientific and Technical Information of China (English)

    张展

    2015-01-01

    目前我国高校图书馆资源共享平台的建设还处于一个起步阶段,建设过程中存在着诸如平台建设参差不齐、共享内容不够全面、平台建设主要依靠数据商、没有建立绩效评价体系等一系列问题。我们需要加强资源建设,进一步扩大共享范围,提升自身形象,强化图书馆在平台建设中的作用,寻求政府支持,完善平台协调管理机制等。%The current university library resources sharing platform in China is still in an early stage of develop-ment.There are still a host of problems facing the construction of resource sharing platform in Chinese university li-braries, such as uneven development in platform construction among different universities, incomprehensive re-sources that can be shared, lop-sided reliance on data business providers, lack of performance evaluation systems, et cetera.To solve these problems, efforts must be made to strengthen library resource building, further expand the scope of sharing, strengthen the role of university libraries in platform construction, enhance libraries’ self-image, seek government support, and improve the mechanism of platform coordination and management.

  1. Digital Libraries

    CERN Document Server

    Papy, Fabrice

    2008-01-01

    Of vital interest to all librarians and information specialists, this book presents all aspects of the effects of digitization of today's and tomorrow's libraries. From social to technical issues, Digital Libraries includes chapters on the growth of the role of librarian, the reader experience, cataloging, search engines, OPAC, law, ergonomic studies, and the future of libraries.

  2. BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes

    Czech Academy of Sciences Publication Activity Database

    Šimková, Hana; Šafář, Jan; Kubaláková, Marie; Suchánková, Pavla; Čihalíková, Jarmila; Robert-Quatre, Heda; Azhaguvel, P.; Weng, Y. Q.; Peng, J.; Lapitan, N. L. V.; Ma, Y. Q.; You, F. M.; Luo, M. C.; Bartoš, Jan; Doležel, Jaroslav

    -, č. 302543 (2011), s. 1-11. ISSN 1110-7243 R&D Projects: GA ČR GA521/07/1573; GA MŠk(CZ) LC06004 Institutional research plan: CEZ:AV0Z50380511 Keywords : TRITICUM-AESTIVUM L. * HEXAPLOID WHEAT * BREAD WHEAT Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.436, year: 2011

  3. Library Outreach: Introducing Campus Childcare Providers to the Academic Library

    Science.gov (United States)

    Edwards, Melissa Maxwell; Thornton, Elaine

    2013-01-01

    This case study describes a library outreach effort to university staff members employed by the campus child care center. Authors planned an instructional session to introduce child care staff members to library resources, focusing on the curriculum collection as a source of supplemental materials for classrooms. Surveys were administered before…

  4. Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01

    International Nuclear Information System (INIS)

    Bacillus subtilis Bac01 was mutated by 15 keV N+ ions of 1.5xl016 cm-2. The mutant strain Bac11 with high yield of endoglucanase was isolated using carboxymethylcellulose sodium and congo red indicative plates. It exhibited higher endoglucanase activity (381.89IU) than the original strain Bac01 (93.33IU). Two 1,500 bp endoglucanase gene fragments were obtained with PCR amplification from B. subtilis Bac01 and mutant strain Bac11. BLAST comparison result indicated that 10 nucleotides mutated. Bioinformatics methods were used to analyze the two predicted amino acid sequences, and it was found that 5 amino acid residues changed, being all in the cellulose-binding domain of endoglucanase. (authors)

  5. Analysis and location of a rice BAC clone containing telomeric DNA sequences

    Institute of Scientific and Technical Information of China (English)

    翟文学; 陈浩; 颜辉煌; 严长杰; 王国梁; 朱立煌

    1999-01-01

    BAC2, a rice BAC clone containing (TTTAGGG)n homologous sequences, was analyzed by Southern hybridization and DNA sequencing of its subclones. It was disclosed that there were many tandem repeated satellite DNA sequences, called TA352, as well as simple tandem repeats consisting of TTTAGGG or its variant within the BAC2 insert. A 0. 8 kb (TTTAGGG) n-containing fragment in BAC2 was mapped in the telomere regions of at least 5 pairs of rice chromosomes by using fluorescence in situ hybridization (FISH). By RFLP analysis of low copy sequences the BAC2 clone was localized in one terminal region of chromosome 6. All the results strongly suggest that the telomeric DNA sequences of rice are TTTAGGG or its variant, and the linked satellite DNA TA352 sequences belong to telomere-associated sequences.

  6. A Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC) Transgenesis in Zebrafish.

    Science.gov (United States)

    Fuentes, Fernando; Reynolds, Eric; Lewellis, Stephen W; Venkiteswaran, Gayatri; Knaut, Holger

    2016-01-01

    Transgenesis of large DNA constructs is essential for gene function analysis. Recently, Tol2 transposase-mediated transgenesis has emerged as a powerful tool to insert bacterial artificial chromosome (BAC) DNA constructs into the genome of zebrafish. For efficient transgenesis, the genomic DNA piece in the BAC construct needs to be flanked by Tol2 transposon sites, and the constructs should contain a transgenesis marker for easy identification of transgenic animals. We report a set of plasmids that contain targeting cassettes that allow the insertion of Tol2 sites and different transgenesis markers into BACs. Using BACs containing these targeting cassettes, we show that transgenesis is as efficient as iTol2, that preselecting for expression of the transgenesis marker increases the transgenesis rate, and that BAC transgenics faithfully recapitulate the endogenous gene expression patterns and allow for the estimation of the endogenous gene expression levels. PMID:26818072

  7. Back to BAC: The Use of Infectious Clone Technologies for Viral Mutagenesis

    Directory of Open Access Journals (Sweden)

    Robyn N. Hall

    2012-02-01

    Full Text Available Bacterial artificial chromosome (BAC vectors were first developed to facilitate the propagation and manipulation of large DNA fragments in molecular biology studies for uses such as genome sequencing projects and genetic disease models. To facilitate these studies, methodologies have been developed to introduce specific mutations that can be directly applied to the mutagenesis of infectious clones (icBAC using BAC technologies. This has resulted in rapid identification of gene function and expression at unprecedented rates. Here we review the major developments in BAC mutagenesis in vitro. This review summarises the technologies used to construct and introduce mutations into herpesvirus icBAC. It also explores developing technologies likely to provide the next leap in understanding these important viruses.

  8. Technological exploration of BAC-FISH on mitotic chromosomes of maize

    Institute of Scientific and Technical Information of China (English)

    Yongsheng TAO; Zuxin ZHANG; Yonglin CHEN; Lijia LI; Yonglian ZHENG

    2008-01-01

    The rice BAC-DNA was used as probes and fluorescence in situ hybridization (FISH) was applied to the interphase and metaphase mitotic chromosomes of maize. To optimize the BAC-FISH technique, we respect-ively assayed the effect of several factors, including maize or rice genomic Cot DNA used as blocking reagent of DNA, washing temperatures and FAD concentration in the washing buffer and in the hybrid solution. The results show that Cot DNA of maize genome blocked the repet-itive sequence of the rice BAC-DNA when the Cot value was below 50. Meanwhile, it was necessary to adjust the Cot value according to the different probes and their ratios. Decreasing the concentration of FAD in the hybridization mixtures, adjusting the washing rate after hybridization, and most especially, blocking the rice-specific repetitive sequences of BAC-DNA could improve the positive signals of BAC-FISH.

  9. Cloning and expression of human UDP-glucuronosyltransferase 1A4 in Bac-to-Bac system

    International Nuclear Information System (INIS)

    UDP-glucuronosyltransferases (UGTs) catalyze the transfer of glucuronic acid from uridine diphosphate-glucuronic acid (UDP-GA) to compounds with amine, hydroxyl, and carboxylic acid moieties. N-glucuronidation is an important pathway for elimination of many tertiary amine therapeutic agents used in humans. UGT1A4 has been reported to be specific for glucuronidating primary, secondary, and tertiary amines, forming N-glucuronides. To further investigate the drugs metabolized by UGT1A4, the Bac-to-Bac expression system was used to express the recombinant UGT1A4 with His-tag on the C-terminal. The His-tagged recombinant UGT1A4 expressed in Spodoptera frugiperda (Sf9) cells were detected using anti-His antibody and the molecular weight of the recombinant protein was approximately 55 kDa. The enzyme activity towards imipramine in cell homogenate protein was found to be 83.14 ± 15 pmol/min/mg protein (n=3) with 0.5 mM imipramine by HPLC, but was not detectable in blank Sf9 cells. It paved the way for the further studies for drug glucuronidation by UGT1A4. The purification of the UGT1A4 can be done by Ni-resin. This is helpful to do research on the structure of the UFT1A4

  10. Libraries Serving the CSIR Complex.

    Science.gov (United States)

    Rajagopalan, T. S.; Ramaswami, K.

    A survey of the resources and services of the Council of Scientific and Industrial Research (CSIR) libraries was made so that the libraries in the complex could share the benefit of the experiences of each other. The report is based on questionnaire replies received from 31 CSIR Institutions and eight Co-operative Research Associations and relates…

  11. JCE Digital Library Grand Opening

    Science.gov (United States)

    Journal of Chemical Education, 2004

    2004-01-01

    The National Science, Technology, Engineering and Mathematical Education Digital Library (NSDL), inaugurated in December 2002, is developed to promote science education on a comprehensive scale. The Journal of Chemical, Education (JCE) Digital Library, incorporated into NSDL, contains its own collections of digital resources for chemistry…

  12. The Weakest Link: Library Catalogs.

    Science.gov (United States)

    Young, Terrence E., Jr.

    2002-01-01

    Describes methods of correcting MARC records in online public access catalogs in school libraries. Highlights include in-house methods; professional resources; conforming to library cataloging standards; vendor services, including Web-based services; software specifically developed for record cleanup; and outsourcing. (LRW)

  13. Chromosome arm-specific BAC end sequences permit comparative analysis of homoeologous chromosomes and genomes of polyploid wheat

    Directory of Open Access Journals (Sweden)

    Sehgal Sunish K

    2012-05-01

    Full Text Available Abstract Background Bread wheat, one of the world’s staple food crops, has the largest, highly repetitive and polyploid genome among the cereal crops. The wheat genome holds the key to crop genetic improvement against challenges such as climate change, environmental degradation, and water scarcity. To unravel the complex wheat genome, the International Wheat Genome Sequencing Consortium (IWGSC is pursuing a chromosome- and chromosome arm-based approach to physical mapping and sequencing. Here we report on the use of a BAC library made from flow-sorted telosomic chromosome 3A short arm (t3AS for marker development and analysis of sequence composition and comparative evolution of homoeologous genomes of hexaploid wheat. Results The end-sequencing of 9,984 random BACs from a chromosome arm 3AS-specific library (TaaCsp3AShA generated 11,014,359 bp of high quality sequence from 17,591 BAC-ends with an average length of 626 bp. The sequence represents 3.2% of t3AS with an average DNA sequence read every 19 kb. Overall, 79% of the sequence consisted of repetitive elements, 1.38% as coding regions (estimated 2,850 genes and another 19% of unknown origin. Comparative sequence analysis suggested that 70-77% of the genes present in both 3A and 3B were syntenic with model species. Among the transposable elements, gypsy/sabrina (12.4% was the most abundant repeat and was significantly more frequent in 3A compared to homoeologous chromosome 3B. Twenty novel repetitive sequences were also identified using de novo repeat identification. BESs were screened to identify simple sequence repeats (SSR and transposable element junctions. A total of 1,057 SSRs were identified with a density of one per 10.4 kb, and 7,928 junctions between transposable elements (TE and other sequences were identified with a density of one per 1.39 kb. With the objective of enhancing the marker density of chromosome 3AS, oligonucleotide primers were successfully designed from

  14. Effects of combined 131I- and CT-BAC5 immunotherapy on NPC CNE-2 multicellular spheroids

    International Nuclear Information System (INIS)

    Objective: To develop a new specific therapeutic method for treating nasopharyngeal carcinoma (NPC) and to evaluate its effects on an in vitro tumor spheroid model. Methods: The human NPC cell line CNE-2 spheroids were grown in culture medium, on a thin layer of 0.75% agarose. The average volume of the spheroids was estimated by 1/2 (short dimension)2 x (long dimension). Anti-NPC BAC5 MAb was labelled with 131I using chloramine-T method. Cytotoxin from Chinese cobra (CT) was conjugated with BAC5 by hetero bifunctional cross-linking reagent (SPDP). The spheroids were treated with either 131I-BAC5 or CT-BAC5 separately or with both of them together by incubating in culture medium. Results: The conjugated rate of BAC5 and CT was 32.4%. CT-BAC5 significantly inhibited the growth of CNE-2 multicellular spheroids comparing with control agent (normal saline) (P 131I-BAC5 did more than CT-BAC5 (P131I-BAC5 with CT-BAC5 treatment showed even stronger destructive effect on the spheroids (vs. normal saline, P 131I-BAC5 with CT-BAC5 is better than either of them working separately

  15. Taking the British Library Forward in the Twenty-First Century; Harvard's Library Digital Initiative: Building a First Generation Digital Library Infrastructure; Spoken Words, Unspoken Meanings: A DLI2 Project Ethnography; Resource Guide for the Social Sciences: Signposting a Dissemination and Support Route for Barefoot and Meta-Librarians in UK Higher Education.

    Science.gov (United States)

    Brindley, Lynne; Flecker, Dale; Seadle, Michael; Huxley, Lesly; Ford, Karen

    2000-01-01

    Includes four articles that discuss strategic planning in the British Library, including electronic strategies and collaborative partnerships; Harvard University's plans for a digital library infrastructure; the National Gallery of the Spoken Word, a Digital Library Initiative (DLI)-funded project that is language-related; and promoting networked…

  16. Library Reference Service.

    Science.gov (United States)

    Schippleck, Suzanne

    The Inglewood, California, public library provides a manual on reference service. The theory, purpose, and objectives of reference are noted, and goals and activities are described in terms of budget, personnel, resources, and services. A chapter on organization covers service structure, information services, relationships with other library…

  17. Homeschoolers in the Library.

    Science.gov (United States)

    Scheps, Susan G.

    1999-01-01

    Discusses the recent growth in homeschooling and offers suggestions for public libraries to support homeschoolers. Highlights include creating your own curricula; publishers who offer resources for homeschoolers; use of the Internet; and examples of books and series, grouped by subject, of materials particularly suited for homeschoolers. (LRW)

  18. Not Your Grandmother's Library!

    Science.gov (United States)

    Perez, Lisa

    2011-01-01

    School libraries are an essential part of a complete school program. They provide an equitable, fiscally responsible strategy for sharing resources across grade levels and the curriculum while addressing core reading, information, and technology literacies. Students are still voracious readers of books in print, and now they consume a variety of…

  19. Chaos in the library environment

    OpenAIRE

    Κατσιρίκου, Ανθή

    2001-01-01

    Describes the impact of chaos theory in social systems and the phenomena that result from it, drawing attention to related phenomena in the state of the library today. Then considers the factors that lead library systems to exhibit chaotic behaviour. These factors are the plethora of technological tools and the variety of software and interfaces, the dependence of resource providers and the increasing supply and diversity of information resources. The changes dictated by these factors influen...

  20. 信息不对称条件下图书馆数字资源利用率问题探析%The utilization rate of library digital resources under the condition of information asymmetry

    Institute of Scientific and Technical Information of China (English)

    李丽娟

    2013-01-01

    书籍是人类进步的阶梯,而图书馆作为图书的主要聚集地,在传播优秀文化、构建和谐社会方面,起着重要的作用。信息不对称普遍存在于图书馆运行的整个过程中,影响图书馆的建设发展和价值实现,本文分析了信息不对称条件下图书馆数字资源利用率现状,并提出了相应的解决措施。%Books are the ladder of human progress, and the library as the main gathering of books, plays an important role in the dissemination of the excellent culture, constructing the harmonious society. Information asymmetry exists in the whole course of library operation, affect the construction and development of library and the realization of the value, this paper analyzes the current situation of utilization of digital library resources under the condition of information asymmetry, and proposes the corresponding solution measures.

  1. Analysis of the Literature Resource Construction of University Library on the Campus Culture%浅析高校图书馆文献资源建设对校园文化的影响

    Institute of Scientific and Technical Information of China (English)

    房华

    2012-01-01

    高校图书馆文献资料具有丰富性和多样性,它能较为全面地支持和服务于不同类型的校园文化。本篇论文通过剖析高校图书馆文献资源对校园文化的影响,阐明了图书馆文化资源直接支撑着各种类型学校文化的有效展开,决定着校园文化总体发展态势和质量水平。%University library literature has a richness and diversity, it can fully support and services in different types of campus culture. This thesis by analyzing the university library literature resources on campus culture, to clarify a library and cultural resources directly support the effective development of all types of school culture to determine the situation and the level of quality of the overall development of the campus culture.

  2. Viability of Adopting Open Source Integrated Library Systems in Indian Libraries: Perspectives of Commercial ILS Users

    OpenAIRE

    Gireesh Kumar, T K

    2015-01-01

    Open source software is becoming first option for automating libraries in most countries even though adoption of open source integrated library system (OSILS) is still at an infant stage in India. OSILS enable libraries to gain more flexibility and freedom in managing and distributing resources than commercial software with license restrictions. The paper examines the perceptions of commercial Integrated Library System (ILS) users on adopting OSILS in Indian libraries. The study identifies th...

  3. Library Locations

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Carnegie Library of Pittsburgh locations including address, coordinates, phone number, square footage, and standard operating hours.

  4. Library of American Civilization Demonstration. Final Report.

    Science.gov (United States)

    Napier, Paul A.; And Others

    In August, 1971, The National Home Library Foundation of Washington, D.C., awarded to The George Washington University a grant to install a set of the ultra-microfiche "Library of American Civilization" (produced by Encyclopaedia Britannica's subsidiary, Library Resources, Inc.), together with all available associated equipment and bibliographic…

  5. The Rise of the Digital Public Library

    Science.gov (United States)

    McKendrick, Joseph

    2012-01-01

    There is a growing shift to digital offerings among public libraries. Libraries increasingly are fulfilling roles as technology hubs for their communities, with high demand for technology and career development training resources. Ebooks and other digital materials are on the rise, while print is being scaled back. More libraries are turning to…

  6. Marketing and Promotion of Library Services

    Science.gov (United States)

    Nicholas, Julie

    As librarians we should be actively marketing and promoting our library services. This paper aims to demystify marketing for librarians. Practical solutions are provided on how to implement a marketing strategy, with particular emphasis on the value of using electronic information resources. It also shows the link between promoting library services and raising the profile of the library.

  7. Conversion of BAC clones into binary BAC (BIBAC) vectors and their delivery into basidiomycete fungal cells using Agrobacterium tumefaciens.

    Science.gov (United States)

    Ali, Shawkat; Bakkeren, Guus

    2015-01-01

    The genetic transformation of certain organisms, required for gene function analysis or complementation, is often not very efficient, especially when dealing with large gene constructs or genomic fragments. We have adapted the natural DNA transfer mechanism from the soil pathogenic bacterium Agrobacterium tumefaciens, to deliver intact large DNA constructs to basidiomycete fungi of the genus Ustilago where they stably integrated into their genome. To this end, Bacterial Artificial Chromosome (BAC) clones containing large fungal genomic DNA fragments were converted via a Lambda phage-based recombineering step to Agrobacterium transfer-competent binary vectors (BIBACs) with a Ustilago-specific selection marker. The fungal genomic DNA fragment was subsequently successfully delivered as T-DNA through Agrobacterium-mediated transformation into Ustilago species where an intact copy stably integrated into the genome. By modifying the recombineering vector, this method can theoretically be adapted for many different fungi. PMID:25239747

  8. BacPP: a web-based tool for Gram-negative bacterial promoter prediction.

    Science.gov (United States)

    de Avila E Silva, S; Notari, D L; Neis, F A; Ribeiro, H G; Echeverrigaray, S

    2016-01-01

    Bacterial Promoter Prediction (BacPP) is a tool used to predict given sequences as promoters of Gram-negative bacteria according to the σ factor that recognizes it. The first version of BacPP was implemented in Python language in a desktop version without a friendly interface. For this reason, a web version of BacPP is now available with the purpose of improving its usability and availability. The present paper describes the implementation of the web version of this tool, focusing on its software architecture and user functionalities. The software is available at www.bacpp.bioinfoucs.com/home. PMID:27173187

  9. BAC: A computer program for calculating shielding in buildings against initial radiation

    Science.gov (United States)

    Danielson, G.

    1980-10-01

    Calculation methodology and transmission data for BAC in the event of a nuclear explosion are considered. The shielding factor is the rate between the radiation dose at one point in the building and the dose in open air. It is separately calculated for neutrons, gamma rays from fission products, and secondary gamma rays. For this calculation, BAC uses data for radiation transmission in concrete. This program is utilized for fallout shelters and other buildings where walls and floors/roofs are mostly made of concrete and bricks. Instructions for the program are given, and BAC results and values are in certain cases compared with those obtained with the Monte Carlo method.

  10. Role of Bacillus subtilis BacB in the Synthesis of Bacilysin

    OpenAIRE

    Rajavel, Malligarjunan; Mitra, Ashima; Gopal, Balasubramanian

    2009-01-01

    Bacilysin is a non-ribosomally synthesized dipeptide antibiotic that is active against a wide range of bacteria and some fungi. Synthesis of bacilysin (L-alanine-[2,3-epoxycyclohexano-4]-L-alanine) is achieved by proteins in the bac operon, also referred to as the bacABCDE (ywfBCDEF) gene cluster in B. subtilis. Extensive genetic analysis from several strains of B. subtilis suggests that the bacABC gene cluster encodes all the proteins that synthesize the epoxyhexanone ring of L-anticapsin...

  11. The Digital Library of the Commons - A Public-Service Space for Resources Sharing%公众共享数字图书馆——国际文献资源共享空间

    Institute of Scientific and Technical Information of China (English)

    刘燕权; 田硕

    2011-01-01

    Designed to be "a gateway to the international literature on the commons", the Digital Library of the Commons is a collaborative project of the Workshop in Political Theory and Policy Analysis and Indiana University Digital Library Program (DLP). The library provides free and open access to full-text articles, papers and dissertations pertaining to the commons, common-pool resources and common property. It also provides space for sharing research on the concept and practical implications of the commons around the world, in physical and virtual communities. The article presents an extended review on the project's backgrounds, resources organization, technological features and services provided Authors' comments and suggestions are also presented for future improvements of the project%公众共享数字图书馆(Digital Library of the Commons)是一个服务公众的资源共享国际文献库,作为印第安纳大学政治理论和政策分析研究室和印第安纳大学数字图书馆项目的一个合作工程,其主要任务是免费向公众提供有关共享资源、公共集中资源和公共财产方面的国际文献.文章从项目概述、资源组织、服务及技术特征等方面对该数字图书馆进行了综合性描述和介绍,同时给出了作者的评价和建议.

  12. Strategic marketing planning in library

    Directory of Open Access Journals (Sweden)

    Karmen Štular-Sotošek

    2000-01-01

    Full Text Available The article is based on the idea that every library can design instruments for creating events and managing the important resources of today's world, especially to manage the changes. This process can only be successful if libraries use adequate marketing methods. Strategic marketing planning starts with the analysis of library's mission, its objectives, goals and corporate culture. By analysing the public environment, the competitive environment and the macro environment, libraries recognise their opportunities and threats. These analyses are the foundations for library definitions: What does the library represent?, What does it aspire to? Which goals does it want to reach? What kind of marketing strategy will it use for its target market?

  13. Library Advocacy

    Science.gov (United States)

    Plunkett, Kate

    2010-01-01

    This paper is about the issue of advocacy. Standing at the vanguard of literacy, library media specialists have a unique role. However, it is time for media specialists to advocate their services in a proactive way. If library media specialists cannot, both individually and collectively, put advocacy at the forefront, then students will suffer the…

  14. Library Use

    DEFF Research Database (Denmark)

    Konzack, Lars

    2012-01-01

    A seminar paper about a survey of role-playing games in public libraries combined with three cases and a presentation of a model.......A seminar paper about a survey of role-playing games in public libraries combined with three cases and a presentation of a model....

  15. Construction and Sharing of Digital Resources of Academic Libraries of China%高校图书馆特色资源的数字化建设与共享

    Institute of Scientific and Technical Information of China (English)

    别立谦; 肖珑; 朱强; 姚晓霞

    2011-01-01

    文章依据CALIS管理中心在全国高校范围内所做的调查,阐述高校图书馆在自建数字资源方面的发展现状,并在此基础上介绍自建资源共享以及CALIS特色库的建设情况,最后以北大图书馆为例,说明单馆开展自建数据库的整体构架和考虑.文章旨在总结现状,为高校图书馆特色资源的未来发展提供参考.%According to the survey among the academic libraries in China held by CALIS Administrative Center in 2010, this paper discussed the development status on self-developed digital resources, and introduced the resource construction and sharing situation of CALIS special collection databases. Taking Peking University Library as an example, this paper also described the considerations on the general structures of self-developed digital collections. Based on the current experiences of resources integration and services improvements, this paper aims to provide the evidences and references to the future development of special collection databases.

  16. Marketing library services to the Net Generation

    Directory of Open Access Journals (Sweden)

    Nadia Haji azizi

    2008-04-01

    Full Text Available This paper aims to examine the role of marketing to new generations of library users. The paper reviews classical marketing texts and current user studies for applicability to library service. The paper finds that libraries can apply classic marketing principles to attract and better serve new generations of users. Although libraries no longer have a monopoly on information sources, libraries do offer value-added services. By understanding the users and their contexts, the paper proposes various strategies of value to market librarians and library resources.

  17. A first generation BAC-based physical map of the channel catfish genome

    Directory of Open Access Journals (Sweden)

    Waldbieser Geoffrey C

    2007-02-01

    Full Text Available Abstract Background Channel catfish, Ictalurus punctatus, is the leading species in North American aquaculture. Genetic improvement of catfish is performed through selective breeding, and genomic tools will help improve selection efficiency. A physical map is needed to integrate the genetic map with the karyotype and to support fine mapping of phenotypic trait alleles such as Quantitative Trait Loci (QTL and the effective positional cloning of genes. Results A genome-wide physical map of the channel catfish was constructed by High-Information-Content Fingerprinting (HICF of 46,548 Bacterial Artificial Chromosomes (BAC clones using the SNaPshot technique. The clones were assembled into contigs with FPC software. The resulting assembly contained 1,782 contigs and covered an estimated physical length of 0.93 Gb. The validity of the assembly was demonstrated by 1 anchoring 19 of the largest contigs to the microsatellite linkage map 2 comparing the assembly of a multi-gene family to Restriction Fragment Length Polymorphism (RFLP patterns seen in Southern blots, and 3 contig sequencing. Conclusion This is the first physical map for channel catfish. The HICF technique allowed the project to be finished with a limited amount of human resource in a high throughput manner. This physical map will greatly facilitate the detailed study of many different genomic regions in channel catfish, and the positional cloning of genes controlling economically important production traits.

  18. 專論/從柏林國立圖書館東亞部看德國國家東亞研究資料資源/考恩 | The East Asia Department of the Berlin State Library: German National Resources for East Asian Materials / Matthias Kaun

    Directory of Open Access Journals (Sweden)

    考恩

    2007-10-01

    Full Text Available

    For more than 50 years the East Asia Department of the Berlin State Library has been hosting a special interest collection on East- and Southeast Asia. Integrated into a federal network of German libraries, supervised and in part financed by the German Research Foundation (DFG – Deutsche Forschungsgemeinschaft, this collection has become the largest of its kind in Europe. The East Asia Department supplies German and European libraries with publications in East Asian languages through a special inter-library loan service. Since 2002 the Berlin State Library has offered access to electronic resources like databases and electronic journals from the East Asian region via the virtual library CrossAsia. Furthermore the East Asia Department has become an access point to East Asian databases for European consortia.

    頁次:9-18

  19. Factors Affecting University Library Website Design

    OpenAIRE

    Kim, Yongi-Mi; University of Oklahoma

    2011-01-01

    Existing studies have extensively explored factors that affect users’ intentions to use university library website resources (ULWR); yet little attention has been given to factors affecting university library website design. This paper investigates factors that affect university library website design and assesses the success of the university library website from both designers’ and users’ perspectives. The findings show that when planning a website, university web designers consider univers...

  20. Performance Indicators for the Digital Library

    OpenAIRE

    2001-01-01

    The purpose of performance indicators is „to assess the quality and effectiveness of services provided by a library ... and to assess the efficiency of resources allocated by the library to such services.” In the last decades, libraries have developed sets of performance indicators for evaluating the quality of their services. Such indicators have been presented in handbooks and in an international standard. They were for the most part restricted to the traditional library services (collectio...

  1. STRATEGIC PLANNING IN THE LIBRARY ENVIRONMENT

    OpenAIRE

    PRADEEP SHRIRAM BANSODE

    2013-01-01

    The use of focus groups to determine patron satisfaction with library resources and services is extensive and well established. This article demonstrates how focus groups can also be used to help shape the future direction of a library as part of the strategic planning process. By responding to questions about their long-term library and information needs, focus group participants at the University of Texas Southwestern Medical Center at Dallas Library contributed an abundance of qualitative ...

  2. Development of the BAC Physical Maps of Wheat Chromosome 6B for Its Genomic Sequencing

    Czech Academy of Sciences Publication Activity Database

    Kobayashi, A.; Katagiri, S.; Karasawa, W.; Takumi, S.; Doležel, Jaroslav; Ogihara, Y.; Handa, H.

    Verlag : Springer, 2015 - (Handa, H.), s. 101-107 ISBN 978-4-431-55674-9 Institutional support: RVO:61389030 Keywords : genome sequencing * wheat * BAC chromosomes Subject RIV: EB - Genetics ; Molecular Biology

  3. Development of the BAC Physical Maps of Wheat Chromosome 6B for Its Genomic Sequencing

    Czech Academy of Sciences Publication Activity Database

    Kobayashi, A.; Katagiri, S.; Karasawa, W.; Takumi, S.; Doležel, Jaroslav; Ogihara, Y.; Handa, H.

    Verlag: Springer, 2015 - (Handa, H.), s. 101-107 ISBN 978-4-431-55674-9 Institutional support: RVO:61389030 Keywords : genome sequencing * wheat * BAC chromosomes Subject RIV: EB - Genetics ; Molecular Biology

  4. BAC-Dkk3-EGFP Transgenic Mouse: An In Vivo Analytical Tool for Dkk3 Expression

    Directory of Open Access Journals (Sweden)

    Yuki Muranishi

    2012-01-01

    Full Text Available Dickkopf (DKK family proteins are secreted modulators of the Wnt signaling pathway and are capable of regulating the development of many organs and tissues. We previously identified Dkk3 to be a molecule predominantly expressed in the mouse embryonic retina. However, which cell expresses Dkk3 in the developing and mature mouse retina remains to be elucidated. To examine the precise expression of the Dkk3 protein, we generated BAC-Dkk3-EGFP transgenic mice that express EGFP integrated into the Dkk3 gene in a BAC plasmid. Expression analysis using the BAC-Dkk3-EGFP transgenic mice revealed that Dkk3 is expressed in retinal progenitor cells (RPCs at embryonic stages and in Müller glial cells in the adult retina. Since Müller glial cells may play a potential role in retinal regeneration, BAC-Dkk3-EGFP mice could be useful for retinal regeneration studies.

  5. Proportional counters as monitoring detectors of BAC chambers at the ZEUS experiment

    International Nuclear Information System (INIS)

    The aim of the investigations presented is the elaboration of the system monitoring working conditions of BAC chambers. The backing calorimeter (BAC) at the ZEUS detector of the HERA accelerator consisting of 5000 proportional chambers of a total volume of 60 m3 is supplied with a gas mixture of Ar+10% CO2 by an open gas system. Flow proportional counters with a 55Fe source are used as gas system monitoring detectors. These counters and sources were designed and made exclusively for that application. The ageing effect of the Ar+CO2 mixture has also been studied. Monitoring measurements of the pulse height distribution enable us to follow the changes of gas pressure and composition in BAC proportional chambers. The results of these measurements will be used for off-line corrections of BAC data. (orig.)

  6. 高校图书馆参与自主MOOC平台资源建设研究%Research on University Libraries Participating in Resource Construction of Self-built MOOC Platforms

    Institute of Scientific and Technical Information of China (English)

    黄月; 任立清

    2015-01-01

    The rapid growth of Massive Open Online Course (MOOC) has brought an important opportunity for change to university libraries. The mode of Chinese universities participating in MOOC includes joining the famous MOOC, building independent MOOC and doing both above ways. This paper first points out university libraries can play a role in following aspects in resource construction of self-built MOOC platforms:accurate resource support, copyright support&consulting and professional service of information literacy. Then proposes a building model of university libraries participating in resource construction of self-built MOOC platforms, which should provide navigation and search functions. Finally, based on practical experience, this paper analyzes the problems and challenges in resource construction of self-built MOOC platforms:multi-sector col aboration, legal access to database, multiple data source integration, high conformity between resources and courses, resource usage data collection and evaluation.%MOOC的蓬勃发展为高校图书馆服务模式变革带来了机遇.国内高校MOOC的实践方式主要有加入知名MOOC、开发自主MOOC以及两种方式并举.文章分析了图书馆在高校自主MOOC平台资源建设中的作用,主要是提供精准资源支撑、版权支持与咨询服务、专业信息素养服务等;重点提出了利用图书馆资源进行高校自主MOOC平台资源建设的建构模型,涵盖资源导航和搜索两大功能.根据实践经验总结了建设过程中的关键点和难点,包括多部门合作、数据库合法调取、多数据源融合、资源与课程高符合度、资源使用数据收集与评估等.

  7. Raising OER Awareness: VT Libraries and Beyond

    OpenAIRE

    Walz, Anita R.

    2014-01-01

    This presentation highlights the first year of exploration into a possible Open Educational Resources (OER) initiative at the University Libraries, Virginia Tech. Key messages to convey while raising OER awareness include: copyright, open licensing, Creative Commons, OER availability and retrieval, adaptation and innovation, understanding one's institution, and pedagogy. Various events and activities of VT Libraries are mentioned in addition to resources for OER librarian support, library cha...

  8. Science and Technology Libraries Partnering with Knovel

    OpenAIRE

    Bhatt, Jay W.; Paulsen, W. Charles; Dunn, Lisa G.; Van Epps, Amy S

    2005-01-01

    The engineering library plays a critical role in initiating and maintaining the conduit between vendors of engineering information and the users of that information—faculty, students, and researchers. Likewise, vendors play a key role in supporting library and faculty efforts to promote engineering information resources, beyond just supplying content and interface. Engineering libraries and vendors can effectively work together to get the most from electronic engineering resources for both ...

  9. Insertional Mutagenesis by a Hybrid PiggyBac and Sleeping Beauty Transposon in the Rat

    OpenAIRE

    Furushima, Kenryo; Jang, Chuan-Wei; Chen, Diane W.; Xiao, Ningna; Overbeek, Paul A.; Behringer, Richard R.

    2012-01-01

    A hybrid piggyBac/Sleeping Beauty transposon-based insertional mutagenesis system that can be mobilized by simple breeding was established in the rat. These transposons were engineered to include gene trap sequences and a tyrosinase (Tyr) pigmentation reporter to rescue the albinism of the genetic background used in the mutagenesis strategy. Single-copy transposon insertions were transposed into the rat genome by co-injection of plasmids carrying the transposon and RNA encoding piggyBac trans...

  10. PiggyBac Transposon Mutagenesis: A Tool for Cancer Gene Discovery in Mice

    OpenAIRE

    Rad, Roland; Rad, Lena; Wang, Wei; Cadinanos, Juan; Vassiliou, George; Rice, Stephen; Campos, Lia S.; Yusa, Kosuke; Banerjee, Ruby; Li, Meng Amy; de la Rosa, Jorge; Strong, Alexander; Lu, Dong; Ellis, Peter; Conte, Nathalie

    2010-01-01

    Transposons are mobile DNA segments that can disrupt gene function by inserting in or near genes. Here we show that insertional mutagenesis by the PiggyBac transposon can be used for cancer gene discovery in mice. PiggyBac transposition in genetically engineered transposon/transposase mice induced cancers whose type (hematopoietic versus solid) and latency were dependent on the regulatory elements introduced into transposons. Analysis of 63 hematopoietic tumors revealed the unique qualities o...

  11. Recombineering strategies for developing next generation BAC transgenic tools for optogenetics and beyond

    OpenAIRE

    Ting, Jonathan T; Guoping Feng

    2014-01-01

    The development and application of diverse BAC transgenic rodent lines has enabled rapid progress for precise molecular targeting of genetically-defined cell types in the mammalian central nervous system. These transgenic tools have played a central role in the optogenetic revolution in neuroscience. Indeed, an overwhelming proportion of studies in this field have made use of BAC transgenic cre driver lines to achieve targeted expression of optogenetic probes in the brain. In addition, sev...

  12. Effect of mea on DNA degradation in permeable irradiated Bac. stearothermophilus cells

    International Nuclear Information System (INIS)

    It was shown that DNA-degrading activity of permeable, intact and γ-irradiated cells of Bac. stearothermophilus decreased under the effect of β-mercaptoethylamine (MEA). MEA decreased also a DNAase activity, in a crude acellular extract of Bac. stearothermophilus, and activities of S1-nuclease and DNAase. I. The data obtained prompt an assumption that MEA has an inhibitory action on the activity of endonucleases irrespective of their substrate specificity

  13. 23 CFR 1225.4 - Adoption of 0.08 BAC per se law.

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Adoption of 0.08 BAC per se law. 1225.4 Section 1225.4... TRANSPORTATION GUIDELINES OPERATION OF MOTOR VEHICLES BY INTOXICATED PERSONS § 1225.4 Adoption of 0.08 BAC per se... is enforcing a law that provides that any person with a blood or breath alcohol concentration...

  14. We Care--Virtually and in Person: A User-Centered Approach to Assessment, Implementation and Promotion of Library Resources and Services to a Remote Graduate Campus

    Science.gov (United States)

    Tremblay, Paul; Wang, Zhonghong

    2008-01-01

    The article relates how the Brooklyn Campus Library of Long Island University, despite limitations of physical distance and the varying conditions of the remote graduate campus at Westchester, makes every effort to face each challenge creatively with practical solutions for implementation. We share our findings and experiences--what worked and…

  15. A Comparison of the Investigation and Curriculum Necessities of the Department of Sociology at Ball State University with Bracken Library Resources. A Research Paper.

    Science.gov (United States)

    Humes, Robert W.

    This paper outlines the procedures for a study that will focus on the information needs of the 12 Department of Sociology faculty members at Ball State University. By comparing needs of the sociology department with current holdings in the library, deficiencies in monograph and serial holdings are expected to be identified. Needs are defined here…

  16. DMCA, CTEA, UCITA ... Oh My! An Overview of Copyright Law and Its Impact on Library Acquisitions and Collection Development of Electronic Resources

    Science.gov (United States)

    Lee, Leslie A.; Wu, Michelle M.

    2007-01-01

    The purpose of traditional copyright law was to encourage the creation of works based on and to ensure reasonable access to original thought. Despite this harmonious intent, an intrinsic tension exists between libraries and copyright holders, as the former promotes "free" access to information that ultimately reduces the income of the latter. The…

  17. Global resource sharing

    CERN Document Server

    Frederiksen, Linda; Nance, Heidi

    2011-01-01

    Written from a global perspective, this book reviews sharing of library resources on a global scale. With expanded discovery tools and massive digitization projects, the rich and extensive holdings of the world's libraries are more visible now than at any time in the past. Advanced communication and transmission technologies, along with improved international standards, present a means for the sharing of library resources around the globe. Despite these significant improvements, a number of challenges remain. Global Resource Sharing provides librarians and library managers with a comprehensive

  18. Measurement Properties of the Low Back Activity Confidence Scale (LoBACS).

    Science.gov (United States)

    Davenport, Todd E; Cleland, Joshua A; Yamada, Kimiko A; Kulig, Kornelia

    2016-06-01

    The purpose of this study was to determine the measurement properties of the Low Back Activity Confidence Scale (LoBACS) in individuals with post-acute low back pain (LBP) receiving nonsurgical intervention, including construct validity, factorial validity, and internal consistency reliability. Data were analyzed from an existing randomized clinical trial involving 112 patients with LBP. Evidence for convergent validity was observed through significant correlations between LoBACS subscale scores and other function, pain, and psychobehavioral measures. LoBACS subscales accounted for 36% of the unique variance in dependent variable measurements, suggesting a satisfactory level of statistical divergence between the LoBACS and other psychobehavioral measurements in this study. Cronbach's α ranged from .88 to .92 for LoBACS subscales, and item-total correlations exceeded .6, indicating high internal consistency reliability. Principal axis factoring confirmed the hypothesized three-subscale structure by correctly classifying 14 of the 15 items. These findings indicate the LoBACS is valid and internally consistent to measure domain-specific self-efficacy beliefs. PMID:24686745

  19. Hybrid nonviral/viral vector systems for improved piggyBac DNA transposon in vivo delivery.

    Science.gov (United States)

    Cooney, Ashley L; Singh, Brajesh K; Sinn, Patrick L

    2015-04-01

    The DNA transposon piggyBac is a potential therapeutic agent for multiple genetic diseases such as cystic fibrosis (CF). Recombinant piggyBac transposon and transposase are typically codelivered by plasmid transfection; however, plasmid delivery is inefficient in somatic cells in vivo and is a barrier to the therapeutic application of transposon-based vector systems. Here, we investigate the potential for hybrid piggyBac/viral vectors to transduce cells and support transposase-mediated genomic integration of the transposon. We tested both adenovirus (Ad) and adeno-associated virus (AAV) as transposon delivery vehicles. An Ad vector expressing hyperactive insect piggyBac transposase (iPB7) was codelivered. We show transposase-dependent transposition activity and mapped integrations in mammalian cells in vitro and in vivo from each viral vector platform. We also demonstrate efficient and persistent transgene expression following nasal delivery of piggyBac/viral vectors to mice. Furthermore, using piggyBac/Ad expressing Cystic Fibrosis transmembrane Conductance Regulator (CFTR), we show persistent correction of chloride current in well-differentiated primary cultures of human airway epithelial cells derived from CF patients. Combining the emerging technologies of DNA transposon-based vectors with well-studied adenoviral and AAV delivery provides new tools for in vivo gene transfer and presents an exciting opportunity to increase the delivery efficiency for therapeutic genes such as CFTR. PMID:25557623

  20. Foreign Literature Resources and the Construction of Public Library in Western Area of China --.Taking Guizhou Provincial Library as An Example%外文文献资料与数字公共图书馆的构建——以贵州省图书馆为例

    Institute of Scientific and Technical Information of China (English)

    张梅

    2012-01-01

    目前我省公共图书馆外文文献资源在收集与利用方面较为薄弱,需对传统的外文文献资源建设模式进行大刀阔斧的改进。贵州省图书馆结合贵州跨越式发展的需要,适时地承担起外文文献资源建设的责任,并取得了一定成效。作为一家省级公共图书馆,在外文文献资源建设上建立了科学的管理体制;加强对信息源的收集,提高采访人员的专业技能,建立与读者长期沟通、交流机制,构建多元化的外文馆藏结构,促进贵州经济发展,为贵州科研发展、文化交流提供了有力保障。%At present, the foreign documentary resources of public library in our province are very weak in the collection and utilization, and need an improvement in the traditional foreign literature resources construction mode. Guizhou Provincial Li brary has achieved some results by combining Guizhou' s great leap forward development of need in time and the responsi bility of foreign literature resources construction. As a provincial public library, Guizhou Provincial Library tries to construct foreign literature resources by establishing a scientific management system, to strengthen the information collection, to improve the staff' s professional skills, to establish long term readers communication mechanism, in this way, we can build a multi ple foreign language structure of the library, and promote economic development in Guizhou, which helps scientific research development in Guizhou cultural communication, and provides a powerful guarantee.

  1. Uncertainty of Blood Alcohol Concentration (BAC Results as Related to Instrumental Conditions: Optimization and Robustness of BAC Analysis Headspace Parameters

    Directory of Open Access Journals (Sweden)

    Haleigh A. Boswell

    2015-12-01

    Full Text Available Analysis of blood alcohol concentration is a routine analysis performed in many forensic laboratories. This analysis commonly utilizes static headspace sampling, followed by gas chromatography combined with flame ionization detection (GC-FID. Studies have shown several “optimal” methods for instrumental operating conditions, which are intended to yield accurate and precise data. Given that different instruments, sampling methods, application specific columns and parameters are often utilized, it is much less common to find information on the robustness of these reported conditions. A major problem can arise when these “optimal” conditions may not also be robust, thus producing data with higher than desired uncertainty or potentially inaccurate results. The goal of this research was to incorporate the principles of quality by design (QBD in the adjustment and determination of BAC (blood alcohol concentration instrumental headspace parameters, thereby ensuring that minor instrumental variations, which occur as a matter of normal work, do not appreciably affect the final results of this analysis. This study discusses both the QBD principles as well as the results of the experiments, which allow for determination of more favorable instrumental headspace conditions. Additionally, method detection limits will also be reported in order to determine a reporting threshold and the degree of uncertainty at the common threshold value of 0.08 g/dL. Furthermore, the comparison of two internal standards, n-propanol and t-butanol, will be investigated. The study showed that an altered parameter of 85 °C headspace oven temperature and 15 psi headspace vial pressurization produces the lowest percent relative standard deviation of 1.3% when t-butanol is implemented as an internal standard, at least for one very common platform. The study also showed that an altered parameter of 100 °C headspace oven temperature and 15-psi headspace vial pressurization

  2. 大學圖書館電子資源之需求分析與行銷策略之研究 A Study of Demands Analysis and Marketing Strategy of Electronic Resources in University Library

    Directory of Open Access Journals (Sweden)

    Huang-Yu Liu

    2008-03-01

    Full Text Available 電子資源的出現,使得知識載體有了新的突破,提高了使用者的便利性與即時性。對於圖書館而言,如何使電子資源的使用效益達到最高,必須思考一套有效的行銷策略。電子資源行銷概念應以讀者為導向,尊重讀者的資訊需求,並利用各種宣傳技巧,行銷電子資源,使圖書館能提供更完善的服務。本研究之目的旨在探討讀者的使用需求及電子資源的推廣策略,以獲致最有效益的行銷方式。研究對象以開南大學日間部四學院(商學院、運輸觀光學院、資訊學院、人文社會學院)學生為樣本,佐以缺口分析模型為分析架構,藉以找出個案中讀者/學生對於電子資源使用的真實需求,並進而歸納出提供服務的大學圖書館在其推廣或行銷服務上可有的因應策略。The emergence of electronic resources has made new breakthrough in knowledge carriers because of their ease of use, instant availability, and the characteristic of no time and space constraints. For public libraries to achieve maximum efficiency in its electronic resources, it is necessary to seek the most effective marketing strategies. Therefore, the marketing concept of the electronic resources should be reader oriented, such as respecting and understanding library user’s information needs. Libraries also need to utilize various media and techniques to market the electronic resources, so that more comprehensive services and experiences can be provided to readers. The purpose of this study is to investigate library user’s needs and promotion strategies of electronic resources in order to identify the most effective ways of marketing. This study focuses on the students of the four colleges (College of Business, College of Tourism, College of Information, and College of Humanities and Social Science in Kainan University as subjects in the survey questionnaire. It uses the Gap Analysis

  3. 公共图书馆数字资源采购、推广与评估%The Study about Procuring,Promoting,Evaluating Digital Resources of Public Library

    Institute of Scientific and Technical Information of China (English)

    刘宝华

    2012-01-01

    This article reviews the principle and mode of the digital resources in the procuring process,post-procurement promotion and evaluation for its use.The result shows that procurement,promotion and evaluation had affected one another,and the coordination of which will help to build complete digital resources of public library.%介绍了数字资源采购过程中的原则和模式,以及数字资源采购之后的推广与效果评估。说明数字资源的采购,推广和评估之间具有相互促进与验证的作用。做好采购、推广和评估等工作有助于构建完整的公共图书馆数字资源。

  4. How Users Search the Library from a Single Search Box

    Science.gov (United States)

    Lown, Cory; Sierra, Tito; Boyer, Josh

    2013-01-01

    Academic libraries are turning increasingly to unified search solutions to simplify search and discovery of library resources. Unfortunately, very little research has been published on library user search behavior in single search box environments. This study examines how users search a large public university library using a prominent, single…

  5. Discovering Jewish Studies Collections in Academic Libraries: A Practical Guide

    Science.gov (United States)

    Taler, Izabella

    2014-01-01

    The U.S. colleges and universities offering non-sectarian educational programs in Jewish Studies rely on the support of their academic libraries for research materials and library services. For college libraries which use Library of Congress Classification scheme, it is a common practice to integrate "studies" resources into their…

  6. Discussion on the Transformation of Library Resources Allocation under the Trend of Low-carbon Reading%论低碳阅读趋势下的馆藏资源配置转型

    Institute of Scientific and Technical Information of China (English)

    王石平

    2012-01-01

    With the popularity of electronic reading, digital resources will gradually become the main col- lection in library. The remote joint storage mechanism should be activated to protect the traditional re- sources of not commonly use but with e-version. In view of e-books' replacing function in library, the reference services department should, by relying on the collection network platform, push forward dif- ferentiated social extension business on the basis of user needs and reinforce the library knowledge hub of information retrieval advisory. Library needs to establish partnership with digital publishing operators to guarantee a convenient and innovative book-lending (download) business. Such socialized commercial cooperation also benefits for sharing electronic paper books.%随着电子阅读的普及,数字资源将逐步成为主力馆藏,图书馆对使用性极小及拥有电子版的传统资源应启动远程联合储存机制。有鉴于电子书在功能上对图书馆已经具有一定的替代作用,图书馆参考咨询部门应倚靠馆藏网络平台,以用户需求为基础推进差别性社会延伸业务,强化图书馆知识信息检索咨询中心的地位。图书馆要与数字出版运营商建立商业合作关系,以便保障新颖便捷的图书借阅(下载)业务,而社会化商业合作也利于图书馆分享电纸书的利益。

  7. Academic Libraries and High-Impact Practices for Student Retention: Library Deans' Perspectives

    Science.gov (United States)

    Murray, Adam

    2015-01-01

    Numerous studies on retention have highlighted the role of student engagement in influencing students' withdrawal decisions. This study seeks to address how academic libraries affect student retention by examining the perception of academic library deans or directors on the alignment between library services and resources with ten nationally…

  8. Library Impact Data Project: Looking for the Link between Library Usage and Student Attainment

    Science.gov (United States)

    Stone, Graham; Ramsden, Bryony

    2013-01-01

    The Library Impact Data Project was a six-month project funded by Jisc and managed by the University of Huddersfield to investigate this hypothesis: "There is a statistically significant correlation across a number of universities between library activity data and student attainment." E-resources usage, library borrowing statistics, and…

  9. China Academic Library and Information System: An Academic Library Consortium in China.

    Science.gov (United States)

    Dai, Longji; Chen, Ling; Zhang, Hongyang

    2000-01-01

    Describes CALIS (China Academic Library and Information System), a nationwide academic library consortium funded primarily by the Chinese government to serve multiple resource-sharing functions among the participating libraries, including online searching, interlibrary loan, document delivery, and coordinated purchasing and cataloging, by…

  10. Design and Implementation of the System of Higher Vocational Library Resources Recommendation Based on Android Platform%基于Android平台的高职图书资源推介系统设计与实现

    Institute of Scientific and Technical Information of China (English)

    吴英宾; 范国渠

    2014-01-01

    In the high-speed wireless network becomes more and more mature and intelligent mobile phone popularization environment,aiming at the promotion of vocational college library resources needs,the paper design and realize a library resources recommendation system based on Android platform.The system is divided into server and Android client,the server through the internet to provide the service interface,and the Android client provides information query,collec tion,management,interaction of readers and other functions.%在高速无线网络日益成熟和智能手机大量普及的环境下,本文针对高职院校图书资源应用的实际需求,设计并实现了一款基于Android平台的图书资源推介系统,系统分为服务器端和Android客户端,服务器端通过互联网提供服务接口,Android客户端提供图书资源推介信息查询、收藏、管理、读者互动等功能。

  11. Ataxin-2 regulates RGS8 translation in a new BAC-SCA2 transgenic mouse model.

    Directory of Open Access Journals (Sweden)

    Warunee Dansithong

    2015-04-01

    Full Text Available Spinocerebellar ataxia type 2 (SCA2 is an autosomal dominant disorder with progressive degeneration of cerebellar Purkinje cells (PCs and other neurons caused by expansion of a glutamine (Q tract in the ATXN2 protein. We generated BAC transgenic lines in which the full-length human ATXN2 gene was transcribed using its endogenous regulatory machinery. Mice with the ATXN2 BAC transgene with an expanded CAG repeat (BAC-Q72 developed a progressive cellular and motor phenotype, whereas BAC mice expressing wild-type human ATXN2 (BAC-Q22 were indistinguishable from control mice. Expression analysis of laser-capture microdissected (LCM fractions and regional expression confirmed that the BAC transgene was expressed in PCs and in other neuronal groups such as granule cells (GCs and neurons in deep cerebellar nuclei as well as in spinal cord. Transcriptome analysis by deep RNA-sequencing revealed that BAC-Q72 mice had progressive changes in steady-state levels of specific mRNAs including Rgs8, one of the earliest down-regulated transcripts in the Pcp2-ATXN2[Q127] mouse line. Consistent with LCM analysis, transcriptome changes analyzed by deep RNA-sequencing were not restricted to PCs, but were also seen in transcripts enriched in GCs such as Neurod1. BAC-Q72, but not BAC-Q22 mice had reduced Rgs8 mRNA levels and even more severely reduced steady-state protein levels. Using RNA immunoprecipitation we showed that ATXN2 interacted selectively with RGS8 mRNA. This interaction was impaired when ATXN2 harbored an expanded polyglutamine. Mutant ATXN2 also reduced RGS8 expression in an in vitro coupled translation assay when compared with equal expression of wild-type ATXN2-Q22. Reduced abundance of Rgs8 in Pcp2-ATXN2[Q127] and BAC-Q72 mice supports our observations of a hyper-excitable mGluR1-ITPR1 signaling axis in SCA2, as RGS proteins are linked to attenuating mGluR1 signaling.

  12. Building Digital Libraries Made Easy: Toward Open Digital Libraries

    OpenAIRE

    Edward A Fox; Suleman, Hussein; Luo, Ming

    2002-01-01

    Digital libraries (DLs) promote a sharing culture among those who contribute and those who use resources. This same approach works when building Open Digital Libraries (ODLs). Leveraging the intellectual and practical investment made in the Open Archives Initiative through an eXtended Protocol for Metadata Harvesting (XPMH), one can build lightweight protocols to tie together key components that together make up the core of a DL. DL developers in various settings have learned how to apply thi...

  13. A Library Response to the Massification of Higher Education: The Case of the University of Zambia Library

    Science.gov (United States)

    Kanyengo, Christine Wamunyima

    2009-01-01

    This paper looks at the challenges that libraries in Africa face in responding to massification of higher education by discussing the University of Zambia library's response in library and information resources provision. As a result of massification of higher education, libraries have been forced not only to employ new and different strategies to…

  14. Introducing the National Library for Health Skin Conditions Specialist Library

    Directory of Open Access Journals (Sweden)

    Williams Hywel C

    2005-04-01

    Full Text Available Abstract Background This paper introduces the new National Library for Health Skin Conditions Specialist Library http://www.library.nhs.uk/skin. Description The aims, scope and audience of the new NLH Skin Conditions Specialist Library, and the composition and functions of its core Project Team, Editorial Team and Stakeholders Group are described. The Library's collection building strategy, resource and information types, editorial policies, quality checklist, taxonomy for content indexing, organisation and navigation, and user interface are all presented in detail. The paper also explores the expected impact and utility of the new Library, as well as some possible future directions for further development. Conclusion The Skin Conditions Specialist Library is not just another new Web site that dermatologists might want to add to their Internet favourites then forget about it. It is intended to be a practical, "one-stop shop" dermatology information service for everyday practical use, offering high quality, up-to-date resources, and adopting robust evidence-based and knowledge management approaches.

  15. 台灣「北區技專校院校際合作聯盟」圖書館資源共享之研究 A Study on Library Resources Sharing NATCU in Taiwan

    Directory of Open Access Journals (Sweden)

    Wei-hwa Chen

    2004-09-01

    Full Text Available 「北區技專校院校際合作聯盟NATCU」於民國91年8月6日在台北技大學舉行締約儀式,由來自台灣北區包含台北、宜蘭、花蓮、新竹及桃園共37所技專校院的校長聯合簽立了承諾書,正式結盟。台灣技專校院在教學研究資源上遠不如綜合大學,有效整合北區技專圖書館資源提供師生共享,將可做為技專校院邁入高等教育之列的策略。當下圖書館聯盟儼然成為世界趨勢。研究結果發現整體性的策略規劃與團體內所有成員的共同努力,獲得教育部經費的補助是該聯盟發展上很重要的第一步,領導階層的勇氣、魄力與決心也是重要關鍵。文末歸納幾點建箴提供大家參考。Northern Alliance of Technical Colleges and Universities (NATCU is a collaborative consortium in the northern region of Taiwan. The ceremony was inaugurated on August 6, 2002 at Taipei Science and Technical University. The cooperative agreement signed by 37 Presidents of the members including the regions of Taipei, Yi-Lan, Hualian, Shin-chu and Tau-yuan. Most technical colleges and universities in Taiwan have far less resources in teaching and research than those of the general universities. To share library resources of the members effectively, it can be a strategy to provide greater resources for their faculty and students, while still being on the rank of higher education. Library Resources sharing is the trend of the world. The fundamental steps of the library consortium are to have an encompassing strategic plan, to have financial aid, and for all the members to participate fully and to the best of their ability. Another important factor in the success of the consortium is strong and decisive leader. Several remarks are inductive at the end in the hope that will have some helps for the NATCU.

  16. Highly Efficient Modification of Bacterial Artificial Chromosomes (BACs) Using Novel Shuttle Vectors Containing the R6Kγ Origin of Replication

    OpenAIRE

    Gong, Shiaoching; Yang, Xiangdong William; Li, Chenjian; Heintz, Nathaniel

    2002-01-01

    Bacterial artificial chromosome (BAC) mediated transgenesis has proven to be a highly reliable way to obtain accurate transgene expression for in vivo studies of gene expression and function. A rate-limiting step in use of this technology to characterize large numbers of genes has been the process with which BACs can be modified by homologous recombination in Escherichia coli. We report here a highly efficient method for modifying BACs by using a novel set of shuttle vectors that contain the ...

  17. A high-resolution physical map integrating an anchored chromosome with the BAC physical maps of wheat chromosome 6B

    OpenAIRE

    Kobayashi, F; Wu, J. Z.; Kanamori, H; Tanaka, T.; Katagiri, S.; Karasawa, W.; Kaneko, S.; Watanabe, S; Sakaguchi, T; Šafář, J. (Jan); Šimková, H. (Hana); Mukai, Y.; M. Hamada; Saito, M; Hayakawa, K

    2015-01-01

    Background: A complete genome sequence is an essential tool for the genetic improvement of wheat. Because the wheat genome is large, highly repetitive and complex due to its allohexaploid nature, the International Wheat Genome Sequencing Consortium (IWGSC) chose a strategy that involves constructing bacterial artificial chromosome (BAC)-based physical maps of individual chromosomes and performing BAC-by-BAC sequencing. Here, we report the construction of a physical map of chromosome 6B with t...

  18. Positive Perceptions of Access to Online Library Resources Correlates with Quality and Quantity of Scholarly Publications among Finnish Academics. A Review of: Vakkari, Pertti. “Perceived Influence of the Use of Electronic Information Resources on Scholarly Work and Publication Productivity.” Journal of the American Society for Information Science and Technology 59.4 (Feb. 15, 2008: 602-12.

    Directory of Open Access Journals (Sweden)

    Scott Marsalis

    2008-12-01

    Full Text Available Objective – To investigate the relationship between academics’ use of library electronic resources and their opinions regarding how these resources have impacted their work, and to investigate the association between this perceived influence and publication productivity during the previous two years.Design – Two specific questions added to an annual online user-survey questionnaire; additional data mined from surveySetting – Twenty-two Finnish Universities served by FinELib, the Finnish Electronic Library.Subjects – Seven hundred and sixty seven academic staff and full-time doctoral students.Methods – A questionnaire was posted in April 2007 on FinELib’s homepage and advertised on each university library’s mainpage, and focused on respondents’ experience in the previous two years. Participants selected answers either from a list of category choices, or, when measuring perceptions, by rating agreement with statements along a four-point scale. Controlled variables measured were the respondents’ academic position, their discipline, membership in a research group, whether their literature use was discipline-specific or interdisciplinary, and their perception of the availability online of the relevant core literature. The independent variable measured was the scholars’ perception of the impact of the use of electronic library resources on their work. The dependent variable measured was the scholars’ self-reported publications in the two years preceding the survey.Main Results – Participants reported a positive impact on the efficiency of their work, most strongly in areas of ease of access, with lesser impacts in the range of materials available to them and the ease with which they can keep up-to-date in their field. To a lesser extent, the scholars perceived a positive impact on the quality of their work. Upon analysis, the study found that access to online library resources improved scholars’ work by the interconnected

  19. NISCAIR: Information Resources

    OpenAIRE

    Waghmode, S. S.

    2012-01-01

    National Institute of Science Communication and Information Resources (NISCAIR) came into existence on 30th Sept. 2002 with the merger of NISCOM and INSDOC. It provides many resources and products to users and researchers i.e.E-Resources,Online Databases, In_house databases, National Science Library, National Knowledge Resources Consortium, etc.

  20. Marketing in One Person Libraries

    OpenAIRE

    Köhrer-Weisser, Sabine

    2011-01-01

    In times of budget restraints and limited capacities it is important to use the available resources strictly market-oriented - in other words focused on the client's needs - and with a view to the company's targets. Even for a One Person Library, a marketing concept is essential in order to optimize the work of the library. This article introduces the different stages of the marketing management process. Hereby, the analysis of strengths and weaknesses and strategic marketing planning build t...

  1. An Leabharlann : The Irish Library

    OpenAIRE

    Sliney, Marjory

    2014-01-01

    ARTICLES Measuring the value of e-resources Fintan Bracken; Community Collecting and World War One: The Europeana 1914–1918 Project K. McSharry; Ulysses and Palmerstown Library: a first reading Siobhan McCrystal and Roslyn Hand; Code of Professional Practice; CONFERENCE REPORTS 1 Online Information 2013 Madelaine Dennison; 2 North/South Symposium on the Value of the School Library Andrew Carlin; 3 IPRIN Conference 2013 Charlotte Cousins; 4 Ready? Read-e Marjo...

  2. BacM, an N-terminally processed bactofilin of Myxococcus xanthus, is crucial for proper cell shape

    OpenAIRE

    Koch, Matthias K.; McHugh, Colleen A; Hoiczyk, Egbert

    2011-01-01

    Bactofilins are fibre-forming bacterial cytoskeletal proteins. Here, we report the structural and biochemical characterization of MXAN_7475 (BacM), one of the four bactofilins of Myxococcus xanthus. Absence of BacM leads to a characteristic ‘crooked’ cell morphology and an increased sensitivity to antibiotics targeting cell wall biosynthesis. The absence of the other three bactofilins MXAN_4637–4635 (BacN-P) has no obvious phenotype. In M. xanthus, BacM exists as a 150-amino-acid full-length ...

  3. Stereochemical Outcome at Four Stereogenic Centers During Conversion of Prephenate to Tetrahydrotyrosine by BacABGF in the Bacilysin Pathway†

    OpenAIRE

    Parker, Jared B.; Walsh, Christopher T.

    2012-01-01

    The first four enzymes of the bacilysin antibiotic pathway, BacABGF, convert prephenate to a tetrahydrotyrosine (H4Tyr) diastereomer on the way to the anticapsin warhead of the dipeptide antibiotic. BacB takes the BacA product endocyclic-Δ4,Δ8-7R-dihydrohydroxyphenylpyruvate (en-H2HPP) and generates a mixture of 3E- and 3Z-olefins of the exocyclic-Δ3,Δ5-dihydrohydroxyphenylpyruvate (ex-H2HPP). The NADH-utilizing BacG then catalyzes a conjugate reduction, adding a pro-S hydride equivalent to C...

  4. Deficiency of a Sinorhizobium meliloti bacA Mutant in Alfalfa Symbiosis Correlates with Alteration of the Cell Envelope

    OpenAIRE

    Ferguson, Gail P.; Roop II, R. Martin; Walker, Graham C.

    2002-01-01

    The BacA protein is essential for the long-term survival of Sinorhizobium meliloti and Brucella abortus within acidic compartments in plant and animal cells, respectively. Since both the S. meliloti and B. abortus bacA mutants have an increased resistance to bleomycin, it was hypothesized that BacA was a transporter of bleomycin and bleomycin-like compounds into the bacterial cell. However, our finding that the S. meliloti bacA mutant also has an increased sensitivity to detergents, a hydroph...

  5. Computational analysis of ordering in non-liquid crystalline versus liquid crystalline materials with special reference to nBAC

    International Nuclear Information System (INIS)

    A computational analysis of ordering in non-liquid crystalline p-n-alkyl benzoic acid, having 1 (1BAC), 2 (2BAC) and 3(3BAC) carbon atoms in the alkyl chain has been carried out with respect to translatory and orientational motions, but detailed results are reported only for 3BAC. The evaluation of net atomic charges and dipole moments at each atomic center has been carried out using complete neglect differential overlap (CNDO/2) method. The modified Rayleigh-Schrodinger perturbation theory along with the multicentered-multipole expansion method has been employed to evaluate long-range interactions, while a '6-exp' potential function has been assumed for short-range interactions. On the basis of stacking, in-plane and terminal interaction energy calculations, all possible arrangements of a molecular pair have been considered. A comparative picture of molecular parameters, such as total energy, binding energy, and total dipole moment of 3BAC with higher homologous series liquid crystalline compounds having 4(4BAC), 5(5BAC), and 6(6BAC) alkyl chain carbon atoms, has been given. It is found that, if a suitable functional group is attached to 3BAC, so that the length to breadth ratio is increased, the molecule will show a change in the long-range order, the phase transition temperature and other liquid crystalline properties.

  6. The Survey on Digital Resources Construction of University Libraries in Hunan Province%湖南省高校图书馆数字资源建设情况调查

    Institute of Scientific and Technical Information of China (English)

    唐妮; 刘罡昊

    2015-01-01

    Through an analysis on the collection situation, databases, costs and network traffic of three Chinese databases of CNKI(China National Knowledge Infrastructure), VIP citation database and WanFang Articles Database,this paper conducts a survey on the total database amount,digital resources acquisition, and digital library project, etc. of the part of university library,and holds that should adopt the pattern of“guided by governments, constructed by enterprises, and shared by colleges and universities”to build a resourceful, diversified,retrieval convenient, comprehensive and unified platform and to build the service mechanism for realizing the co-construction and sharing of digital resources.%通过对中国知网、维普资讯和万方数据库三大中文数据库的收录情况、费用及网络流量的分析,对部分高校图书馆拥有数据库总量、数字资源采购、数字化图书馆项目等进行了调查,认为应该采用“政府引导,企业建设,院校共享”的方式建设一个资源丰富、多样化、检索便捷、全面的统一平台,实现数字化资源的共建共享服务机制。

  7. COMBINATORIAL LIBRARIES

    DEFF Research Database (Denmark)

    1997-01-01

    The invention provides a method for the production of a combinatorial library of compound of general formula (I) using solid phase methodologies. The cleavage of the array of immobilised compounds of the phthalimido type from the solid support matrix is accomplished by using an array of dinucleop......The invention provides a method for the production of a combinatorial library of compound of general formula (I) using solid phase methodologies. The cleavage of the array of immobilised compounds of the phthalimido type from the solid support matrix is accomplished by using an array of...... dinucleophiles, e.g. hydrazines (hydrazinolysis) or N-hydroxylamines, whereby a combinatorial dimension is introduced in the cleavage step. The invention also provides a compound library....

  8. Library management for the digital age a new paradigm

    CERN Document Server

    Todaro, Julie

    2014-01-01

    This revolutionary introduction to library management is the first conceived in and written for a digital age. Library Management for the Digital Age covers hierarchies, policies, communication, working relationships, facilities, human resources, settings, customer services, budgeting, and emergency management.

  9. National Library of Medicine Guide to Finding Health Information

    Science.gov (United States)

    ... FAQs > FAQ: Reference & Consumer Health Questions FAQ: National Library of Medicine® Guide to Finding Health Information NLM ... can suggest health information resources. Contents What National Library of Medicine (NLM / National Institutes of Health (NIH) ...

  10. PiggyBac transposon mutagenesis: a tool for cancer gene discovery in mice.

    Science.gov (United States)

    Rad, Roland; Rad, Lena; Wang, Wei; Cadinanos, Juan; Vassiliou, George; Rice, Stephen; Campos, Lia S; Yusa, Kosuke; Banerjee, Ruby; Li, Meng Amy; de la Rosa, Jorge; Strong, Alexander; Lu, Dong; Ellis, Peter; Conte, Nathalie; Yang, Fang Tang; Liu, Pentao; Bradley, Allan

    2010-11-19

    Transposons are mobile DNA segments that can disrupt gene function by inserting in or near genes. Here, we show that insertional mutagenesis by the PiggyBac transposon can be used for cancer gene discovery in mice. PiggyBac transposition in genetically engineered transposon-transposase mice induced cancers whose type (hematopoietic versus solid) and latency were dependent on the regulatory elements introduced into transposons. Analysis of 63 hematopoietic tumors revealed that PiggyBac is capable of genome-wide mutagenesis. The PiggyBac screen uncovered many cancer genes not identified in previous retroviral or Sleeping Beauty transposon screens, including Spic, which encodes a PU.1-related transcription factor, and Hdac7, a histone deacetylase gene. PiggyBac and Sleeping Beauty have different integration preferences. To maximize the utility of the tool, we engineered 21 mouse lines to be compatible with both transposon systems in constitutive, tissue- or temporal-specific mutagenesis. Mice with different transposon types, copy numbers, and chromosomal locations support wide applicability. PMID:20947725

  11. El enfoque de competencias en la formación de recursos humanos para bibliotecas Competencies focus in human resources education for libraries

    Directory of Open Access Journals (Sweden)

    Lina Escalona Ríos

    2008-12-01

    Full Text Available El presente trabajo aborda el enfoque de competencias en la formación de recursos humanos para las bibliotecas, particularmente en los programas de capacitación que pueden aprovechar este enfoque en tanto que optimiza el desarrollo de las destrezas y competencias adecuadas para trabajar en una biblioteca. El enfoque de competencias se aplicó a la actualización de la Capacitación en Biblioteconomía del Colegio de Bachilleres de la Secretaría de Educación Pública, que tiene entre sus finalidades capacitar a los estudiantes para el trabajo, además de darles una formación propedéutica para continuar con estudios universitarios.A competencies focus is discussed particularly with regard to program training to optimize the development of adequate skills and competency for library work. This focus was used to update training in Library Science word at SEP's (Public Education Ministry Colegio de Bachilleres, whose purpose is to prepare students for the work place, besides giving them propaedeutic instruction to continue on with university studies.

  12. Evidence Based Research Activities, Interests and Opportunities Exist for Practitioners in all Library Sectors in the British Isles. A review of: McNicol, Sarah. “Is Research an Untapped Resource in the Library and Information Profession?” Journal of Librarianship and Information Science 36.3 (September 2004):119-26.

    OpenAIRE

    Julie McKenna

    2006-01-01

    Objective – To scope the range, nature and challenges of present, planned and future research by practitioners within libraries in the British Isles. Design – A series of survey questionnaires sent by mail. Setting – Public, academic, health, special and school libraries of the British Isles. Subjects – A total of 2384 questionnaires were sent out and 334 responses were received. 62 academic libraries, 83 health libraries, 78 public libraries, 63 school libraries and 48 special li...

  13. Developing a peer to peer library support scheme

    OpenAIRE

    Coombs, Jenny; Hollier, Carol

    2014-01-01

    The article describes a University of Nottingham scheme in which science and engineering students have been acting as library student ambassadors (Learning Resource Leaders) to engage their peers on library issues. The project was based upon the premise that science and engineering students make less use of their library resources than other disciplines, and began with a project to employ library student ambassadors, funded by a Teaching Innovation Award at Loughborough Univers...

  14. News from the Library

    CERN Multimedia

    CERN Library

    2010-01-01

    A third of the world’s current literature in electrical engineering is available on your CERN desktop Looking for a technical standard on software reviews and audits? Is it referred to as "IEEE color books"? Want to download and read NOW the latest version of IEEE 802? Whenever a need for a technical standard or specification arises in your activity, the Library is here to serve you. For IEEE standards it is particularly easy; the whole collection is available for immediate download. Indeed, since 2007, the CERN Library offers readers online access to the complete IEEE Electronic Library (Institute of Electrical and Electronics Engineers). This licence gives unlimited online access to all IEEE and IET journals and proceedings, starting from the first issue. But not everyone knows that this resource gives also access to all current IEEE standards as well as a selection of archival ones. The Library is now working on the integration of a selection of these standards in our onlin...

  15. Application of RFID Technology in Libraries and Role of Librarian

    OpenAIRE

    Mahajan, Kartika D.; Pandey, Prabhat; Pandher, Balbir Kaur

    2010-01-01

    Library consist intellectual capital it might be scholarly journals, books, reports, theses etc. For security purpose, the goal of the security system should be to provide a safe and secure facility for library employees, library resources and equipment and library patrons. At the same time due to application of security system, that promise to increase efficiency, productivity and enhance user satisfaction. Considering the importance of library security, the present paper concentrates on...

  16. Imagine a National Digital Library: I Wonder If We Can

    OpenAIRE

    French, AL

    2011-01-01

    Keynote talk about the National Digital Public Library planning initiative given at the Electronic Resources and Libraries Annual Meeting in Austin, TX. The paper discusses the history of Carnegie libraries in establishing a public library system in the United States and the establishment of the Korean National Digital Library as a building as well as an online presence in Seoul. true (Invited?) Final version as presented

  17. Construction and characterization of bacterial artificial chromosomes (BACs) containing herpes simplex virus full-length genomes.

    Science.gov (United States)

    Nagel, Claus-Henning; Pohlmann, Anja; Sodeik, Beate

    2014-01-01

    Bacterial artificial chromosomes (BACs) are suitable vectors not only to maintain the large genomes of herpesviruses in Escherichia coli but also to enable the traceless introduction of any mutation using modern tools of bacterial genetics. To clone a herpes simplex virus genome, a BAC replication origin is first introduced into the viral genome by homologous recombination in eukaryotic host cells. As part of their nuclear replication cycle, genomes of herpesviruses circularize and these replication intermediates are then used to transform bacteria. After cloning, the integrity of the recombinant viral genomes is confirmed by restriction length polymorphism analysis and sequencing. The BACs may then be used to design virus mutants. Upon transfection into eukaryotic cells new herpesvirus strains harboring the desired mutations can be recovered and used for experiments in cultured cells as well as in animal infection models. PMID:24671676

  18. Enterprise Reference Library

    Science.gov (United States)

    Bickham, Grandin; Saile, Lynn; Havelka, Jacque; Fitts, Mary

    2011-01-01

    Introduction: Johnson Space Center (JSC) offers two extensive libraries that contain journals, research literature and electronic resources. Searching capabilities are available to those individuals residing onsite or through a librarian s search. Many individuals have rich collections of references, but no mechanisms to share reference libraries across researchers, projects, or directorates exist. Likewise, information regarding which references are provided to which individuals is not available, resulting in duplicate requests, redundant labor costs and associated copying fees. In addition, this tends to limit collaboration between colleagues and promotes the establishment of individual, unshared silos of information The Integrated Medical Model (IMM) team has utilized a centralized reference management tool during the development, test, and operational phases of this project. The Enterprise Reference Library project expands the capabilities developed for IMM to address the above issues and enhance collaboration across JSC. Method: After significant market analysis for a multi-user reference management tool, no available commercial tool was found to meet this need, so a software program was built around a commercial tool, Reference Manager 12 by The Thomson Corporation. A use case approach guided the requirements development phase. The premise of the design is that individuals use their own reference management software and export to SharePoint when their library is incorporated into the Enterprise Reference Library. This results in a searchable user-specific library application. An accompanying share folder will warehouse the electronic full-text articles, which allows the global user community to access full -text articles. Discussion: An enterprise reference library solution can provide a multidisciplinary collection of full text articles. This approach improves efficiency in obtaining and storing reference material while greatly reducing labor, purchasing and

  19. The Optimization of the Resources Allocation of Library ’s RFID System Based on Queuing Theory%基于排队论的图书馆RFID系统的资源配置优化

    Institute of Scientific and Technical Information of China (English)

    申晓静

    2015-01-01

    Through calculating readers’ queuing time, average waiting time and other parameters, and under the principle of ensuring the supremacy of reader service, the queuing theory model is applied into the modern library that has Introduced the FRID, which can save money, maximize the utilization of equipment, and more pontifically and more rationally optimize the resources allocation of modern library that has introduced FRID technique.%通过对读者排队时间及平均等待时间等参数的计算,在保证读者服务至上的原则下,将排队论模型应用于引进了FRID技术的现代化图书馆,可节约资金,使设备的利用率最大化,更科学、合理地对引进了FRID技术的现代化图书馆进行资源配置优化。

  20. Comparative sequence and genetic analyses of asparagus BACs reveal no microsynteny with onion or rice.

    Science.gov (United States)

    Jakse, Jernej; Telgmann, Alexa; Jung, Christian; Khar, Anil; Melgar, Sergio; Cheung, Foo; Town, Christopher D; Havey, Michael J

    2006-12-01

    The Poales (includes the grasses) and Asparagales [includes onion (Allium cepa L.) and asparagus (Asparagus officinalis L.)] are the two most economically important monocot orders. The Poales are a member of the commelinoid monocots, a group of orders sister to the Asparagales. Comparative genomic analyses have revealed a high degree of synteny among the grasses; however, it is not known if this synteny extends to other major monocot groups such as the Asparagales. Although we previously reported no evidence for synteny at the recombinational level between onion and rice, microsynteny may exist across shorter genomic regions in the grasses and Asparagales. We sequenced nine asparagus BACs to reveal physically linked genic-like sequences and determined their most similar positions in the onion and rice genomes. Four of the asparagus BACs were selected using molecular markers tightly linked to the sex-determining M locus on chromosome 5 of asparagus. These BACs possessed only two putative coding regions and had long tracts of degenerated retroviral elements and transposons. Five asparagus BACs were selected after hybridization of three onion cDNAs that mapped to three different onion chromosomes. Genic-like sequences that were physically linked on the cDNA-selected BACs or genetically linked on the M-linked BACs showed significant similarities (e asparagus and rice across these regions. Genic-like sequences that were linked in asparagus were used to identify highly similar (e asparagus and genetic linkages in onion. These results further indicate that synteny among grass genomes does not extend to a sister order in the monocots and that asparagus may not be an appropriate smaller genome model for plants in the Asparagales with enormous nuclear genomes. PMID:17016688