WorldWideScience

Sample records for bac library resource

  1. Library Resources for Bac End Sequencing. Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Pieter J. de Jong

    2000-10-01

    Studies directed towards the specific aims outlined for this research award are summarized. The RPCI II Human Bac Library has been expanded by the addition of 6.9-fold genomic coverage. This segment has been generated from a MBOI partial digest of the same anonymous donor DNA used for the rest of the library. A new cloning vector, pTARBAC1, has been constructed and used in the construction of RPCI-II segment 5. This new cloning vector provides a new strategy in identifying targeted genomic regions and will greatly facilitate a large-scale analysis for positional cloning. A new maleCS7BC/6J mouse BAC library has been constructed. RPCI-23 contain 576 plates (approx 210,000 clones) and represents approximately 11-fold coverage of the mouse genome.

  2. A comprehensive BAC resource

    OpenAIRE

    Zhao, Shaying

    2001-01-01

    The Human Genome Project has generated extensive map and sequence data for a large number of Bacterial Artificial Chromosome (BAC) clones. In order to maximize the efficient use of the data and to minimize the redundant work for the research community, The Institute for Genomic Research (TIGR) comprehensive BAC resource (cBACr) (http://www.tigr.org/tdb/BacResource/BAC_resource_intro.html) was built as an expansion of the TIGR human BAC ends database. This resource coll...

  3. Toward an Integrated BAC Library Resource for Genome Sequencing and Analysis; FINAL

    International Nuclear Information System (INIS)

    We developed a great deal of expertise in building large BAC libraries from a variety of DNA sources including humans, mice, corn, microorganisms, worms, and Arabidopsis. We greatly improved the technology for screening these libraries rapidly and for selecting appropriate BACs and mapping BACs to develop large overlapping contigs. We became involved in supplying BACs and BAC contigs to a variety of sequencing and mapping projects and we began to collaborate with Drs. Adams and Venter at TIGR and with Dr. Leroy Hood and his group at University of Washington to provide BACs for end sequencing and for mapping and sequencing of large fragments of chromosome 16. Together with Dr. Ian Dunham and his co-workers at the Sanger Center we completed the mapping and they completed the sequencing of the first human chromosome, chromosome 22. This was published in Nature in 1999 and our BAC contigs made a major contribution to this sequencing effort. Drs. Shizuya and Ding invented an automated highly accurate BAC mapping technique. We also developed long-term collaborations with Dr. Uli Weier at UCSF in the design of BAC probes for characterization of human tumors and specific chromosome deletions and breakpoints. Finally the contribution of our work to the human genome project has been recognized in the publication both by the international consortium and the NIH of a draft sequence of the human genome in Nature last year. Dr. Shizuya was acknowledged in the authorship of that landmark paper. Dr. Simon was also an author on the Venter/Adams Celera project sequencing the human genome that was published in Science last year

  4. Toward an Integrated BAC Library Resource for Genome Sequencing and Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Simon, M. I.; Kim, U.-J.

    2002-02-26

    We developed a great deal of expertise in building large BAC libraries from a variety of DNA sources including humans, mice, corn, microorganisms, worms, and Arabidopsis. We greatly improved the technology for screening these libraries rapidly and for selecting appropriate BACs and mapping BACs to develop large overlapping contigs. We became involved in supplying BACs and BAC contigs to a variety of sequencing and mapping projects and we began to collaborate with Drs. Adams and Venter at TIGR and with Dr. Leroy Hood and his group at University of Washington to provide BACs for end sequencing and for mapping and sequencing of large fragments of chromosome 16. Together with Dr. Ian Dunham and his co-workers at the Sanger Center we completed the mapping and they completed the sequencing of the first human chromosome, chromosome 22. This was published in Nature in 1999 and our BAC contigs made a major contribution to this sequencing effort. Drs. Shizuya and Ding invented an automated highly accurate BAC mapping technique. We also developed long-term collaborations with Dr. Uli Weier at UCSF in the design of BAC probes for characterization of human tumors and specific chromosome deletions and breakpoints. Finally the contribution of our work to the human genome project has been recognized in the publication both by the international consortium and the NIH of a draft sequence of the human genome in Nature last year. Dr. Shizuya was acknowledged in the authorship of that landmark paper. Dr. Simon was also an author on the Venter/Adams Celera project sequencing the human genome that was published in Science last year.

  5. Development of genomic resources for Citrus clementina: Characterization of three deep-coverage BAC libraries and analysis of 46,000 BAC end sequences

    Directory of Open Access Journals (Sweden)

    Talon Manuel

    2008-09-01

    Full Text Available Abstract Background Citrus species constitute one of the major tree fruit crops of the subtropical regions with great economic importance. However, their peculiar reproductive characteristics, low genetic diversity and the long-term nature of tree breeding mostly impair citrus variety improvement. In woody plants, genomic science holds promise of improvements and in the Citrus genera the development of genomic tools may be crucial for further crop improvements. In this work we report the characterization of three BAC libraries from Clementine (Citrus clementina, one of the most relevant citrus fresh fruit market cultivars, and the analyses of 46.000 BAC end sequences. Clementine is a diploid plant with an estimated haploid genome size of 367 Mb and 2n = 18 chromosomes, which makes feasible the use of genomics tools to boost genetic improvement. Results Three genomic BAC libraries of Citrus clementina were constructed through EcoRI, MboI and HindIII digestions and 56,000 clones, representing an estimated genomic coverage of 19.5 haploid genome-equivalents, were picked. BAC end sequencing (BES of 28,000 clones produced 28.1 Mb of genomic sequence that allowed the identification of the repetitive fraction (12.5% of the genome and estimation of gene content (31,000 genes of this species. BES analyses identified 3,800 SSRs and 6,617 putative SNPs. Comparative genomic studies showed that citrus gene homology and microsyntheny with Populus trichocarpa was rather higher than with Arabidopsis thaliana, a species phylogenetically closer to citrus. Conclusion In this work, we report the characterization of three BAC libraries from C. clementina, and a new set of genomic resources that may be useful for isolation of genes underlying economically important traits, physical mapping and eventually crop improvement in Citrus species. In addition, BAC end sequencing has provided a first insight on the basic structure and organization of the citrus genome and has

  6. End Sequencing and Finger Printing of Human & Mouse BAC Libraries

    Energy Technology Data Exchange (ETDEWEB)

    Fraser, C

    2005-09-27

    This project provided for continued end sequencing of existing and new BAC libraries constructed to support human sequencing as well as to initiate BAC end sequencing from the mouse BAC libraries constructed to support mouse sequencing. The clones, the sequences, and the fingerprints are now an available resource for the community at large. Research and development of new metaodologies for BAC end sequencing have reduced costs and increase throughput.

  7. Development of genomic resources for the prairie vole (Microtus ochrogaster: construction of a BAC library and vole-mouse comparative cytogenetic map

    Directory of Open Access Journals (Sweden)

    Young Larry J

    2010-01-01

    Full Text Available Abstract Background The prairie vole (Microtus ochrogaster is a premier animal model for understanding the genetic and neurological basis of social behaviors. Unlike other biomedical models, prairie voles display a rich repertoire of social behaviors including the formation of long-term pair bonds and biparental care. However, due to a lack of genomic resources for this species, studies have been limited to a handful of candidate genes. To provide a substrate for future development of genomic resources for this unique model organism, we report the construction and characterization of a bacterial artificial chromosome (BAC library from a single male prairie vole and a prairie vole-mouse (Mus musculus comparative cytogenetic map. Results We constructed a prairie vole BAC library (CHORI-232 consisting of 194,267 recombinant clones with an average insert size of 139 kb. Hybridization-based screening of the gridded library at 19 loci established that the library has an average depth of coverage of ~10×. To obtain a small-scale sampling of the prairie vole genome, we generated 3884 BAC end-sequences totaling ~2.8 Mb. One-third of these BAC-end sequences could be mapped to unique locations in the mouse genome, thereby anchoring 1003 prairie vole BAC clones to an orthologous position in the mouse genome. Fluorescence in situ hybridization (FISH mapping of 62 prairie vole clones with BAC-end sequences mapping to orthologous positions in the mouse genome was used to develop a first-generation genome-wide prairie vole-mouse comparative cytogenetic map. While conserved synteny was observed between this pair of rodent genomes, rearrangements between the prairie vole and mouse genomes were detected, including a minimum of five inversions and 16 inter-chromosomal rearrangements. Conclusions The construction of the prairie vole BAC library and the vole-mouse comparative cytogenetic map represent the first genome-wide modern genomic resources developed for this

  8. Construction of the BAC Library of Small Abalone (Haliotis diversicolor) for Gene Screening and Genome Characterization.

    Science.gov (United States)

    Jiang, Likun; You, Weiwei; Zhang, Xiaojun; Xu, Jian; Jiang, Yanliang; Wang, Kai; Zhao, Zixia; Chen, Baohua; Zhao, Yunfeng; Mahboob, Shahid; Al-Ghanim, Khalid A; Ke, Caihuan; Xu, Peng

    2016-02-01

    The small abalone (Haliotis diversicolor) is one of the most important aquaculture species in East Asia. To facilitate gene cloning and characterization, genome analysis, and genetic breeding of it, we constructed a large-insert bacterial artificial chromosome (BAC) library, which is an important genetic tool for advanced genetics and genomics research. The small abalone BAC library includes 92,610 clones with an average insert size of 120 Kb, equivalent to approximately 7.6× of the small abalone genome. We set up three-dimensional pools and super pools of 18,432 BAC clones for target gene screening using PCR method. To assess the approach, we screened 12 target genes in these 18,432 BAC clones and identified 16 positive BAC clones. Eight positive BAC clones were then sequenced and assembled with the next generation sequencing platform. The assembled contigs representing these 8 BAC clones spanned 928 Kb of the small abalone genome, providing the first batch of genome sequences for genome evaluation and characterization. The average GC content of small abalone genome was estimated as 40.33%. A total of 21 protein-coding genes, including 7 target genes, were annotated into the 8 BACs, which proved the feasibility of PCR screening approach with three-dimensional pools in small abalone BAC library. One hundred fifty microsatellite loci were also identified from the sequences for marker development in the future. The BAC library and clone pools provided valuable resources and tools for genetic breeding and conservation of H. diversicolor.

  9. Construction of BAC Libraries from Flow-Sorted Chromosomes.

    Science.gov (United States)

    Šafář, Jan; Šimková, Hana; Doležel, Jaroslav

    2016-01-01

    Cloned DNA libraries in bacterial artificial chromosome (BAC) are the most widely used form of large-insert DNA libraries. BAC libraries are typically represented by ordered clones derived from genomic DNA of a particular organism. In the case of large eukaryotic genomes, whole-genome libraries consist of a hundred thousand to a million clones, which make their handling and screening a daunting task. The labor and cost of working with whole-genome libraries can be greatly reduced by constructing a library derived from a smaller part of the genome. Here we describe construction of BAC libraries from mitotic chromosomes purified by flow cytometric sorting. Chromosome-specific BAC libraries facilitate positional gene cloning, physical mapping, and sequencing in complex plant genomes. PMID:27511172

  10. BAC Library of T. pallidum DNA in E. coli

    OpenAIRE

    Šmajs, David; McKevitt, Matthew; Wang, Ling; Howell, Jerrilyn K.; Norris, Steven J; Palzkill, Timothy; Weinstock, George M.

    2002-01-01

    Treponema pallidum subspecies pallidum (Nichols) chromosomal DNA was used to construct a large insert bacterial artificial chromosome (BAC) library in Escherichia coli DH10B using the pBeloBAC11 cloning vector; 678 individual insert termini of 339 BAC clones (13.9 x coverage) were sequenced and the cloned chromosomal region in each clone was determined by comparison to the genomic sequence. A single 15.6-kb region of the T. pallidum chromosome was missing in the BAC library, between bp 248727...

  11. Construction and characterization of a bovine BAC library with four genome-equivalent coverage

    Directory of Open Access Journals (Sweden)

    Eilertsen Ken

    2001-09-01

    Full Text Available Abstract A bovine artificial chromosome (BAC library of 105 984 clones has been constructed in the vector pBeloBAC11 and organized in 3-dimension pools and high density membranes for screening by PCR and hybridization. The average insert size, determined after analysis of 388 clones, was estimated at 120 kb corresponding to a four genome coverage. Given the fact that a male was used to construct the library, the probability of finding any given autosomal and X or Y locus is respectively 0.98 and 0.86. The library was screened for 164 microsatellite markers and an average of 3.9 superpools was positive for each PCR system. None of the 50 or so BAC clones analysed by FISH was chimeric. This BAC library increases the international genome coverage for cattle to around 28 genome equivalents and extends the coverage of the ruminant genomes available at the Inra resource center to 15 genome equivalents.

  12. Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, Lates calcarifer

    Directory of Open Access Journals (Sweden)

    Lin Grace

    2008-03-01

    Full Text Available Abstract Background Barramundi (Lates calcarifer is an important farmed marine food fish species. Its first generation linkage map has been applied to map QTL for growth traits. To identify genes located in QTL responsible for specific traits, genomic large insert libraries are of crucial importance. We reported herein a bacterial artificial chromosome (BAC library and the mapping of BAC clones to the linkage map. Results This BAC library consisted of 49,152 clones with an average insert size of 98 kb, representing 6.9-fold haploid genome coverage. Screening the library with 24 microsatellites and 15 ESTs/genes demonstrated that the library had good genome coverage. In addition, 62 novel microsatellites each isolated from 62 BAC clones were mapped onto the first generation linkage map. A total of 86 BAC clones were anchored on the linkage map with at least one BAC clone on each linkage group. Conclusion We have constructed the first BAC library for L. calcarifer and mapped 86 BAC clones to the first generation linkage map. This BAC library and the improved linkage map with 302 DNA markers not only supply an indispensable tool to the integration of physical and linkage maps, the fine mapping of QTL and map based cloning genes located in QTL of commercial importance, but also contribute to comparative genomic studies and eventually whole genome sequencing.

  13. Construction of a BAC library from cucumber (Cucumis sativus L.) and identification of linkage group specific clones

    Institute of Scientific and Technical Information of China (English)

    Yuan Guan; Qi Chen; Junsong Pan; Zheng Li; Huanle He; Aizhong Wu; Rentao Song; Run Cai

    2008-01-01

    A bacterial artificial chromosome (BAC) library consisting of 19,200 clones with an average insert size of 105 kb has been constructed from a cucumber (Cucumis sativus L.) inbred line S94, derived from a cultivar in North China. The entire library was equivalent to approximately 5 haploid cucumber genomes. To facilitate chromosome engineering and anchor the cucumber genetic linkage map to its chromosomes, 15 sequence-characterized amplified regions (SCAR) and seven simple sequence repeats (SSR) markers from each link-age group of cucumber were used to screen an ordered array of pooled BAC DNA with polymerase chain reaction (PCR). Fifteen mark-ers gave at least two positive clones. As a result, 22 BAC clones representing 7 linkage groups of cucumber were identified, which further validated the genome coverage and utility of the library. This BAC library and linkage group specific clones provide essential resources for future research of the cucumber genome.

  14. Advancing Eucalyptus genomics: identification and sequencing of lignin biosynthesis genes from deep-coverage BAC libraries

    Directory of Open Access Journals (Sweden)

    Kudrna David

    2011-03-01

    Full Text Available Abstract Background Eucalyptus species are among the most planted hardwoods in the world because of their rapid growth, adaptability and valuable wood properties. The development and integration of genomic resources into breeding practice will be increasingly important in the decades to come. Bacterial artificial chromosome (BAC libraries are key genomic tools that enable positional cloning of important traits, synteny evaluation, and the development of genome framework physical maps for genetic linkage and genome sequencing. Results We describe the construction and characterization of two deep-coverage BAC libraries EG_Ba and EG_Bb obtained from nuclear DNA fragments of E. grandis (clone BRASUZ1 digested with HindIII and BstYI, respectively. Genome coverages of 17 and 15 haploid genome equivalents were estimated for EG_Ba and EG_Bb, respectively. Both libraries contained large inserts, with average sizes ranging from 135 Kb (Eg_Bb to 157 Kb (Eg_Ba, very low extra-nuclear genome contamination providing a probability of finding a single copy gene ≥ 99.99%. Libraries were screened for the presence of several genes of interest via hybridizations to high-density BAC filters followed by PCR validation. Five selected BAC clones were sequenced and assembled using the Roche GS FLX technology providing the whole sequence of the E. grandis chloroplast genome, and complete genomic sequences of important lignin biosynthesis genes. Conclusions The two E. grandis BAC libraries described in this study represent an important milestone for the advancement of Eucalyptus genomics and forest tree research. These BAC resources have a highly redundant genome coverage (> 15×, contain large average inserts and have a very low percentage of clones with organellar DNA or empty vectors. These publicly available BAC libraries are thus suitable for a broad range of applications in genetic and genomic research in Eucalyptus and possibly in related species of Myrtaceae

  15. A highly redundant BAC library of Atlantic salmon (Salmo salar: an important tool for salmon projects

    Directory of Open Access Journals (Sweden)

    Koop Ben F

    2005-04-01

    library. We have demonstrated the library's ability to identify specific genes and genetic markers using hybridization, PCR and fingerprinting experiments. In addition, multiple fingerprinting contigs indicated a pseudo-tetraploidity of the Atlantic salmon genome. The highly redundant CHORI-214 BAC library is expected to be an important resource for mapping and sequencing of the Atlantic salmon genome.

  16. Enhancing genome investigations in the mosquito Culex quinquefasciatus via BAC library construction and characterization

    Directory of Open Access Journals (Sweden)

    Saski Christopher A

    2011-09-01

    Full Text Available Abstract Background Culex quinquefasciatus (Say is a major species in the Culex pipiens complex and an important vector for several human pathogens including West Nile virus and parasitic filarial nematodes causing lymphatic filariasis. It is common throughout tropical and subtropical regions and is among the most geographically widespread mosquito species. Although the complete genome sequence is now available, additional genomic tools are needed to improve the sequence assembly. Findings We constructed a bacterial artificial chromosome (BAC library using the pIndigoBAC536 vector and HindIII partially digested DNA isolated from Cx. quinquefasciatus pupae, Johannesburg strain (NDJ. Insert size was estimated by NotI digestion and pulsed-field gel electrophoresis of 82 randomly selected clones. To estimate genome coverage, each 384-well plate was pooled for screening with 29 simple sequence repeat (SSR and five gene markers. The NDJ library consists of 55,296 clones arrayed in 144 384-well microplates. Fragment insert size ranged from 50 to 190 kb in length (mean = 106 kb. Based on a mean insert size of 106 kb and a genome size of 579 Mbp, the BAC library provides ~10.1-fold coverage of the Cx. quinquefasciatus genome. PCR screening of BAC DNA plate pools for SSR loci from the genetic linkage map and for four genes associated with reproductive diapause in Culex pipiens resulted in a mean of 9.0 positive plate pools per locus. Conclusion The NDJ library represents an excellent resource for genome assembly enhancement and characterization in Culex pipiens complex mosquitoes.

  17. Construction and characterization of a Lipotes vexillifer genomic DNA BAC library.

    Science.gov (United States)

    Du, Bo; Zhang, Xian-Feng; Fang, Sheng-Guo; Wang, Ding

    2007-04-01

    We constructed a genomic DNA library for Lipotes vexillifer (L. vexillifer), the Baiji or Yangtze River dolphin, one of the most endangered mammals in the world. The library consists of 149,000 BAC clones, with an average insert size of 83 kb, representing approximately 3.4 haploid genome equivalents. PCR amplification of four known L. vexillifer genes yielded two to four positive clones each. To demonstrate the utility of this library, we isolated and sequenced the L. vexillifer alpha lactalbumin gene, which is a gene specific to mammals and one which has been widely used as molecular tool in phylogenetic analysis. We also end-sequenced 20 randomly selected clones, resulting in the identification of at least five new L. vexillifer genes, five SSR loci, and one SINE locus. These results suggest that this library is a valuable resource for candidate gene cloning, physical mapping, and genome sequencing of this important and threatened species. PMID:17867838

  18. Construction and sequence sampling of deep-coverage, large-insert BAC libraries for three model lepidopteran species

    Directory of Open Access Journals (Sweden)

    Zhao Shaying

    2009-06-01

    Full Text Available Abstract Background Manduca sexta, Heliothis virescens, and Heliconius erato represent three widely-used insect model species for genomic and fundamental studies in Lepidoptera. Large-insert BAC libraries of these insects are critical resources for many molecular studies, including physical mapping and genome sequencing, but not available to date. Results We report the construction and characterization of six large-insert BAC libraries for the three species and sampling sequence analysis of the genomes. The six BAC libraries were constructed with two restriction enzymes, two libraries for each species, and each has an average clone insert size ranging from 152–175 kb. We estimated that the genome coverage of each library ranged from 6–9 ×, with the two combined libraries of each species being equivalent to 13.0–16.3 × haploid genomes. The genome coverage, quality and utility of the libraries were further confirmed by library screening using 6~8 putative single-copy probes. To provide a first glimpse into these genomes, we sequenced and analyzed the BAC ends of ~200 clones randomly selected from the libraries of each species. The data revealed that the genomes are AT-rich, contain relatively small fractions of repeat elements with a majority belonging to the category of low complexity repeats, and are more abundant in retro-elements than DNA transposons. Among the species, the H. erato genome is somewhat more abundant in repeat elements and simple repeats than those of M. sexta and H. virescens. The BLAST analysis of the BAC end sequences suggested that the evolution of the three genomes is widely varied, with the genome of H. virescens being the most conserved as a typical lepidopteran, whereas both genomes of H. erato and M. sexta appear to have evolved significantly, resulting in a higher level of species- or evolutionary lineage-specific sequences. Conclusion The high-quality and large-insert BAC libraries of the insects, together

  19. Construction and characterization of two BAC libraries representing a deep-coverage of the genome of chicory (Cichorium intybus L., Asteraceae)

    OpenAIRE

    Gonthier Lucy; Bellec Arnaud; Blassiau Christelle; Prat Elisa; Helmstetter Nicolas; Rambaud Caroline; Huss Brigitte; Hendriks Theo; Bergès Hélène; Quillet Marie-Christine

    2010-01-01

    Abstract Background The Asteraceae represents an important plant family with respect to the numbers of species present in the wild and used by man. Nonetheless, genomic resources for Asteraceae species are relatively underdeveloped, hampering within species genetic studies as well as comparative genomics studies at the family level. So far, six BAC libraries have been described for the main crops of the family, i.e. lettuce and sunflower. Here we present the characterization of BAC libraries ...

  20. Construction and characterization of two Citrus BAC libraries and identification of clones containing the phytoene synthase gene.

    Science.gov (United States)

    Baig, M N R; Yu, An; Guo, Wenwu; Deng, Xiuxin

    2009-05-01

    Two deep-coverage Bacterial Artificial Chromosome (BAC) libraries of Citrus sinensis (L.) Osbeck 'Cara Cara' navel orange and Citrus reticulata (L.) Blanco 'Egan No. 1' Ponkan mandarin, which belong to the two most important species of the Citrus genus, have been constructed and characterized to facilitate gene cloning and to analyze variety-specific genome composition. The C. sinensis BAC library consists of 36 000 clones with negligible false-positive clones and an estimated average insert size of 126 kb covering ~4.5 x 109 bp and thus providing an 11.8-fold coverage of haploid genome equivalents, whereas the C. reticulata library consists of 21 000 clones also with negligible false-positive clones and an estimated average of 120 kb covering ~2.5 x 109 bp representing a 6.6-fold coverage of haploid genome equivalents. Both libraries were evaluated for contamination with high-copy vector, empty pIndigoBAC536 vector, and organellar DNA sequences. Screening has been performed by Southern hybridization of BAC filters, which results in genomics research in the two important species C. sinensis and C. reticulata. Resources, high-density filters, individual clones, and whole libraries are available for public distribution and are accessible at the National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University.

  1. Physical Analysis of the Complex Rye (Secale cereale L.) Alt4 Aluminium (Aluminum) Tolerance Locus Using a Whole-Genome BAC Library of Rye cv. Blanco

    Science.gov (United States)

    Rye is a diploid crop species with many outstanding qualities, and is also important as a source of new traits for wheat and triticale improvement. Here we describe a BAC library of rye cv. Blanco, representing a valuable resource for rye molecular genetic studies. The library provides a 6 × genome ...

  2. The first insight into the salvia (lamiaceae) genome via bac library construction and high-throughput sequencing of target bac clones

    International Nuclear Information System (INIS)

    Salvia is a representative genus of Lamiaceae, a eudicot family with significant species diversity and population adaptibility. One of the key goals of Salvia genomics research is to identify genes of adaptive significance. This information may help to improve the conservation of adaptive genetic variation and the management of medicinal plants to increase their health and productivity. Large-insert genomic libraries are a fundamental tool for achieving this purpose. We report herein the construction, characterization and screening of a gridded BAC library for Salvia officinalis (sage). The S. officinalis BAC library consists of 17,764 clones and the average insert size is 107 Kb, corresponding to 3 haploid genome equivalents. Seventeen positive clones (average insert size 115 Kb) containing five terpene synthase (TPS) genes were screened out by PCR and 12 of them were subject to Illumina HiSeq 2000 sequencing, which yielded 28,097,480 90-bp raw reads (2.53 Gb). Scaffolds containing sabinene synthase (Sab), a Sab homolog, TPS3 (kaurene synthase-like 2), copalyl diphosphate synthase 2 and one cytochrome P450 gene were retrieved via de novo assembly and annotation, which also have flanking noncoding sequences, including predicted promoters and repeat sequences. Among 2,638 repeat sequences, there are 330 amplifiable microsatellites. This BAC library provides a new resource for Lamiaceae genomic studies, including microsatellite marker development, physical mapping, comparative genomics and genome sequencing. Characterization of positive clones provided insights into the structure of the Salvia genome. These sequences will be used in the assembly of a future genome sequence for S. officinalis. (author)

  3. Construction, Characterization, and Preliminary BAC-End Sequence Analysis of a Bacterial Artificial Chromosome Library of the Tea Plant (Camellia sinensis

    Directory of Open Access Journals (Sweden)

    Jinke Lin

    2011-01-01

    Full Text Available We describe the construction and characterization of a publicly available BAC library for the tea plant, Camellia sinensis. Using modified methods, the library was constructed with the aim of developing public molecular resources to advance tea plant genomics research. The library consists of a total of 401,280 clones with an average insert size of 135 kb, providing an approximate coverage of 13.5 haploid genome equivalents. No empty vector clones were observed in a random sampling of 576 BAC clones. Further analysis of 182 BAC-end sequences from randomly selected clones revealed a GC content of 40.35% and low chloroplast and mitochondrial contamination. Repetitive sequence analyses indicated that LTR retrotransposons were the most predominant sequence class (86.93%–87.24%, followed by DNA retrotransposons (11.16%–11.69%. Additionally, we found 25 simple sequence repeats (SSRs that could potentially be used as genetic markers.

  4. Construction and characterization of a BAC library for the molecular dissection of a single wild beet centromere and sugar beet (Beta vulgaris) genome analysis.

    Science.gov (United States)

    Gindullis, F; Dechyeva, D; Schmidt, T

    2001-10-01

    We have constructed a sugar beet bacterial artificial chromosome (BAC) library of the chromosome mutant PRO1. This Beta vulgaris mutant carries a single chromosome fragment of 6-9 Mbp that is derived from the wild beet Beta procumbens and is transmitted efficiently in meiosis and mitosis. The library consists of 50,304 clones, with an average insert size of 125 kb. Filter hybridizations revealed that approximately 3.1% of the clones contain mitochondrial or chloroplast DNA. Based on a haploid genome size of 758 Mbp, the library represents eight genome equivalents. Thus, there is a greater than 99.96% probability that any sequence of the PROI genome can be found in the library. Approximately 0.2% of the clones hybridized with centromeric sequences of the PRO1 minichromosome. Using the identified BAC clones in fluorescence in situ hybridization experiments with PRO1 and B. procumbens chromosome spreads, their wild-beet origin and centromeric localization were demonstrated. Comparative Southern hybridization of pulsed-field separated PROI DNA and BAC inserts indicate that the centromeric region of the minichromosome is represented by overlapping clones in the library. Therefore, the PRO1 BAC library provides a useful tool for the characterization of a single plant centromere and is a valuable resource for sugar beet genome analysis.

  5. A BAC library of Beta vulgaris L. for the targeted isolation of centromeric DNA and molecular cytogenetics of Beta species.

    Science.gov (United States)

    Jacobs, Gunnar; Dechyeva, Daryna; Wenke, Torsten; Weber, Beatrice; Schmidt, Thomas

    2009-03-01

    We constructed a sugar beet (Beta vulgaris) bacterial artificial chromosome (BAC) library of the monosomic addition line PAT2. This chromosomal mutant carries a single additional chromosome fragment (minichromosome) derived from the wild beet Beta patellaris. Restriction analysis of the mutant line by pulsed-field gel electrophoresis was used to determine HindIII as a suitable enzyme for partial digestion of genomic DNA to generate large-insert fragments which were cloned into the vector pCC1. The library consists of 36,096 clones with an average insert size of 120 kb, and 2.2% of the clones contain mitochondrial or chloroplast DNA. Based on a haploid genome size of 758 Mbp, the library represents 5.7 genome equivalents providing the probability of 99.67% that any sequence of the PAT2 genome can be found in the library. Hybridization to high-density filters was used to isolate 89 BACs containing arrays of the centromere-associated satellite repeats pTS5 and pTS4.1. Using the identified BAC clones in fluorescent in situ hybridization experiments with PAT2 and Beta patellaris chromosome spreads their wild beet origin and centromeric localization was demonstrated. Multi-colour FISH with differently labelled satellite repeats pTS5 and pTS4.1 was used to investigate the large-scale organization of the centromere of the PAT2 minichromosome in detail. FISH studies showed that the centromeric satellite pTS5 is flanked on both sides by pTS4.1 arrays and the arms of the minichromosome are terminated by the Arabidopsis-type telomeric sequences. FISH with a BAC, selected from high-density filters after hybridization with an RFLP marker of the genetic linkage group I, demonstrated that it is feasible to correlate genetic linkage groups with chromosomes. Therefore, the PAT2 BAC library provides a useful tool for the characterization of Beta centromeres and a valuable resource for sugar beet genome analysis.

  6. BAC Library Construction and Physical Mapping of Bacillus anthracis A16R

    Institute of Scientific and Technical Information of China (English)

    Zhang Da; Zhu Houchu; Huang Liuyu

    2013-01-01

    Bacillus anthracis is an endospore-forming bacterium that causes severe inhalational anthrax, and bacillus anthracis A16R is an attenuated strain derived from Bacillus anthracis A16. The development of bacterial artificial chromosome (BAC) system has allowed the construction of large insert-size DNA libraries, and the bacterial artificial chromosomes (BACs) have become the preferred large insert cloning system for genomic analysis because such libraries are characteristically stable, high in ifdelity and easy to handle. To facilitate genome studies of this bacterium, a bacterial artiifcial chromosome library (BAC) has been established from genome DNA of Bacillus anthracis A16R. This library consisted of 9 600 clones randomly selected from more than 15 000 recombinant clones carrying inserts in the plindigoBAC-5 vectors. The mean insert size was 56 kbp, representing an approximate 12-fold genome coverage, while end sequences were obtained from 700 randomly selected clones. Sequences were compared with Bacillus anthracis Ames and Bacillus cereus ATCC 14579 Genome Project databases using the NCBI BLASTN search project. And most BLASTN results showed high identities and that the sequences’ sites could be used as STSs. To construct this physical map, Excel was used for the array of STSs and some gaps of the map were iflled up by PCR walking. Artemis-V4 was used in the construction of a genome-wide physical map with 93%genome coverage. The A16R BAC library proved to be a vital tool for the generation of a map that would not only allow the subsequent sequencing of defined areas of genome, but also provide immediate access to clones that were stable and convenient for functional genomic researches.

  7. BAC Library Construction and Physical Mapping of Bacillus anthracis A16R

    Directory of Open Access Journals (Sweden)

    Da Zhang

    2013-12-01

    Full Text Available Bacillus anthracis is an endospore-forming bacterium that causes severe inhalational anthrax, and bacillus anthracis A16R is an attenuated strain derived from Bacillus anthracis A16. The development of bacterial artificial chromosome (BAC system has allowed the construction of large insert-size DNA libraries, and the bacterial artificial chromosomes (BACs have become the preferred large insert cloning system for genomic analysis because such libraries are characteristically stable, high in fidelity and easy to handle. To facilitate genome studies of this bacterium, a bacterial artificial chromosome library (BAC has been established from genome DNA of Bacillus anthracis A16R. This library consisted of 9 600 clones randomly selected from more than 15 000 recombinant clones carrying inserts in the plindigoBAC-5 vectors. The mean insert size was 56 kbp, representing an approximate 12-fold genome coverage, while end sequences were obtained from 700 randomly selected clones. Sequences were compared with Bacillus anthracis Ames and Bacillus cereus ATCC 14579 Genome Project databases using the NCBI BLASTN search project. And most BLASTN results showed high identities and that the sequences’ sites could be used as STSs. To construct this physical map, Excel was used for the array of STSs and some gaps of the map were filled up by PCR walking. Artemis-V4 was used in the construction of a genome-wide physical map with 93% genome coverage. The A16R BAC library proved to be a vital tool for the generation of a map that would not only allow the subsequent sequencing of defined areas of genome, but also provide immediate access to clones that were stable and convenient for functional genomic researches.

  8. Versatile P(acman) BAC Libraries for Transgenesis Studies in Drosophila melanogaster

    Energy Technology Data Exchange (ETDEWEB)

    Venken, Koen J.T.; Carlson, Joseph W.; Schulze, Karen L.; Pan, Hongling; He, Yuchun; Spokony, Rebecca; Wan, Kenneth H.; Koriabine, Maxim; de Jong, Pieter J.; White, Kevin P.; Bellen, Hugo J.; Hoskins, Roger A.

    2009-04-21

    We constructed Drosophila melanogaster BAC libraries with 21-kb and 83-kb inserts in the P(acman) system. Clones representing 12-fold coverage and encompassing more than 95percent of annotated genes were mapped onto the reference genome. These clones can be integrated into predetermined attP sites in the genome using Phi C31 integrase to rescue mutations. They can be modified through recombineering, for example to incorporate protein tags and assess expression patterns.

  9. Technology at Washington University School of Medicine Library: BACS, PHILSOM, and OCTANET.

    OpenAIRE

    Crawford, S.; M.F. Johnson; Kelly, E. A.

    1983-01-01

    A brief overview of the Bibliographic Access and Control System developed by the Washington University School of Medicine Library is presented. Because the system has been described in two previous reports, this paper focuses on its relationship to other automated programs (i.e., PHILSOM and OCTANET), education of users, evaluation of the system, and outreach to the medical center. In operation for more than two years, BACS represents the computerization of much of the managerial and operatio...

  10. Library resources on the Internet

    Science.gov (United States)

    Buchanan, Nancy L.

    1995-07-01

    Library resources are prevalent on the Internet. Library catalogs, electronic books, electronic periodicals, periodical indexes, reference sources, and U.S. Government documents are available by telnet, Gopher, World Wide Web, and FTP. Comparatively few copyrighted library resources are available freely on the Internet. Internet implementations of library resources can add useful features, such as full-text searching. There are discussion lists, Gophers, and World Wide Web pages to help users keep up with new resources and changes to existing ones. The future will bring more library resources, more types of library resources, and more integrated implementations of such resources to the Internet.

  11. Study on the Mitochondrial Genome of Sea Island Cotton (Gossypium barbadense) by BAC Library Screening

    Institute of Scientific and Technical Information of China (English)

    SU Ai-guo; LI Shuang-shuang; LIU Guo-zheng; LEI Bin-bin; KANG Ding-ming; LI Zhao-hu; MA Zhi-ying; HUA Jin-ping

    2014-01-01

    The plant mitochondrial genome displays complex features, particularly in terms of cytoplasmic male sterility (CMS). Therefore, research on the cotton mitochondrial genome may provide important information for analyzing genome evolution and exploring the molecular mechanism of CMS. In this paper, we present a preliminary study on the mitochondrial genome of sea island cotton (Gossypium barbadense) based on positive clones from the bacterial artiifcial chromosome (BAC) library. Thirty-ifve primers designed with the conserved sequences of functional genes and exons of mitochondria were used to screen positive clones in the genome library of the sea island cotton variety called Pima 90-53. Ten BAC clones were obtained and veriifed for further study. A contig was obtained based on six overlapping clones and subsequently laid out primarily on the mitochondrial genome. One BAC clone, clone 6 harbored with the inserter of approximate 115 kb mtDNA sequence, in which more than 10 primers fragments could be ampliifed, was sequenced and assembled using the Solexa strategy. Fifteen mitochondrial functional genes were revealed in clone 6 by gene annotation. The characteristics of the syntenic gene/exon of the sequences and RNA editing were preliminarily predicted.

  12. A new implementation of high-throughput five-dimensional clone pooling strategy for BAC library screening

    Directory of Open Access Journals (Sweden)

    Deal Karin R

    2010-12-01

    Full Text Available Abstract Background A five-dimensional (5-D clone pooling strategy for screening of bacterial artificial chromosome (BAC clones with molecular markers utilizing highly-parallel Illumina GoldenGate assays and PCR facilitates high-throughput BAC clone and BAC contig anchoring on a genetic map. However, this strategy occasionally needs manual PCR to deconvolute pools and identify truly positive clones. Results A new implementation is reported here for our previously reported clone pooling strategy. Row and column pools of BAC clones are divided into sub-pools with 1~2× genome coverage. All BAC pools are screened with Illumina's GoldenGate assay and the BAC pools are deconvoluted to identify individual positive clones. Putative positive BAC clones are then further analyzed to find positive clones on the basis of them being neighbours in a contig. An exhaustive search or brute force algorithm was designed for this deconvolution and integrated into a newly developed software tool, FPCBrowser, for analyzing clone pooling data. This algorithm was used with empirical data for 55 Illumina GoldenGate SNP assays detecting SNP markers mapped on Aegilops tauschii chromosome 2D and Ae. tauschii contig maps. Clones in single contigs were successfully assigned to 48 (87% specific SNP markers on the map with 91% precision. Conclusion A new implementation of 5-D BAC clone pooling strategy employing both GoldenGate assay screening and assembled BAC contigs is shown here to be a high-throughput, low cost, rapid, and feasible approach to screening BAC libraries and anchoring BAC clones and contigs on genetic maps. The software FPCBrowser with the integrated clone deconvolution algorithm has been developed and is downloadable at http://avena.pw.usda.gov/wheatD/fpcbrowser.shtml.

  13. Construction of an Americn mink Bacterial Artificial Chromosome (BAC) library and sequencing candidate genes important for the fur industry

    DEFF Research Database (Denmark)

    Anistoroaei, Razvan Marian; Hallers, Boudewijn ten; Nefedov, Michael;

    2011-01-01

    contigs (184 kb in average) were assembled. Knowing the complete sequences of these candidate genes will enable confirmation of the association with a phenotype and the finding of causative mutations for the targeted phenotypes.Additionally, 1577 BAC clones were end sequenced; 2505 BAC end sequences (80......BACKGROUND: Bacterial artificial chromosome (BAC) libraries continue to be invaluable tools for the genomic analysis of complex organisms. Complemented by the newly and fast growing deep sequencing technologies, they provide an excellent source of information in genomics projects. RESULTS: Here, we...... consisting of 18,432 clones spotted in duplicate, have been produced for hybridization screening and are publicly available. Overgo probes derived from expressed sequence tags (ESTs), representing 21 candidate genes for traits important for the mink industry, were used to screen the BAC library...

  14. Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus

    Directory of Open Access Journals (Sweden)

    Wilson Melanie

    2003-11-01

    Full Text Available Abstract A bacterial artificial chromosome (BAC library was constructed by cloning HindIII-digested high molecular weight DNA from a gynogenetic channel catfish, Ictalurus punctatus, into the vector pBeloBAC11. Approximately 53 500 clones were arrayed in 384-well plates and stored at -80°C (CCBL1, while clones from a smaller insert size fraction were stored at -80°C without arraying (CCBL2. Pulsed-field gel electrophoresis of 100 clones after NotI digestion revealed an average insert size of 165 kb for CCBL1 and 113 kb for CCBL2. Further characterization of CCBL1 demonstrated that 10% of the clones did not contain an insert. CCBL1 provides a 7.2-fold coverage of the channel catfish haploid genome. PCR-based screening demonstrated that 68 out of 74 unique loci were present in the library. This represents a 92% chance to find a unique sequence. These libraries will be useful for physical mapping of the channel catfish genome, and identification of genes controlling major traits in this economically important species.

  15. BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut

    Directory of Open Access Journals (Sweden)

    Chaine Christian

    2008-01-01

    Full Text Available Abstract Background Cultivated peanut, Arachis hypogaea is an allotetraploid of recent origin, with an AABB genome. In common with many other polyploids, it seems that a severe genetic bottle-neck was imposed at the species origin, via hybridisation of two wild species and spontaneous chromosome duplication. Therefore, the study of the genome of peanut is hampered both by the crop's low genetic diversity and its polyploidy. In contrast to cultivated peanut, most wild Arachis species are diploid with high genetic diversity. The study of diploid Arachis genomes is therefore attractive, both to simplify the construction of genetic and physical maps, and for the isolation and characterization of wild alleles. The most probable wild ancestors of cultivated peanut are A. duranensis and A. ipaënsis with genome types AA and BB respectively. Results We constructed and characterized two large-insert libraries in Bacterial Artificial Chromosome (BAC vector, one for each of the diploid ancestral species. The libraries (AA and BB are respectively c. 7.4 and c. 5.3 genome equivalents with low organelle contamination and average insert sizes of 110 and 100 kb. Both libraries were used for the isolation of clones containing genetically mapped legume anchor markers (single copy genes, and resistance gene analogues. Conclusion These diploid BAC libraries are important tools for the isolation of wild alleles conferring resistances to biotic stresses, comparisons of orthologous regions of the AA and BB genomes with each other and with other legume species, and will facilitate the construction of a physical map.

  16. Construction of a nurse shark (Ginglymostoma cirratum bacterial artificial chromosome (BAC library and a preliminary genome survey

    Directory of Open Access Journals (Sweden)

    Inoko Hidetoshi

    2006-05-01

    Full Text Available Abstract Background Sharks are members of the taxonomic class Chondrichthyes, the oldest living jawed vertebrates. Genomic studies of this group, in comparison to representative species in other vertebrate taxa, will allow us to theorize about the fundamental genetic, developmental, and functional characteristics in the common ancestor of all jawed vertebrates. Aims In order to obtain mapping and sequencing data for comparative genomics, we constructed a bacterial artificial chromosome (BAC library for the nurse shark, Ginglymostoma cirratum. Results The BAC library consists of 313,344 clones with an average insert size of 144 kb, covering ~4.5 × 1010 bp and thus providing an 11-fold coverage of the haploid genome. BAC end sequence analyses revealed, in addition to LINEs and SINEs commonly found in other animal and plant genomes, two new groups of nurse shark-specific repetitive elements, NSRE1 and NSRE2 that seem to be major components of the nurse shark genome. Screening the library with single-copy or multi-copy gene probes showed 6–28 primary positive clones per probe of which 50–90% were true positives, demonstrating that the BAC library is representative of the different regions of the nurse shark genome. Furthermore, some BAC clones contained multiple genes, making physical mapping feasible. Conclusion We have constructed a deep-coverage, high-quality, large insert, and publicly available BAC library for a cartilaginous fish. It will be very useful to the scientific community interested in shark genomic structure, comparative genomics, and functional studies. We found two new groups of repetitive elements specific to the nurse shark genome, which may contribute to the architecture and evolution of the nurse shark genome.

  17. A Bac Library and Paired-PCR Approach to Mapping and Completing the Genome Sequence of Sulfolobus Solfataricus P2

    DEFF Research Database (Denmark)

    She, Qunxin; Confalonieri, F.; Zivanovic, Y.;

    2000-01-01

    screening. The PCR approaches included a novel chromosome walking method termed “paired-PCR”. 21 gaps were filled by BAC end sequence analyses and 6 gaps were filled by PCR including three large ones by paired-PCR. The complete map revealed that 0.9 Mb remained to be sequenced and 34 BAC clones were......The original strategy used in the Sulfolobus solfatnricus genome project was to sequence non overlapping, or minimally overlapping, cosmid or lambda inserts without constructing a physical map. However, after only about two thirds of the genome sequence was completed, this approach became counter......-productive because there was a high sequence bias in the cosmid and lambda libraries. Therefore, a new approach was devised for linking the sequenced regions which may be generally applicable. BAC libraries were constructed and terminal sequences of the clones were determined and used for both end mapping and PCR...

  18. Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library

    Directory of Open Access Journals (Sweden)

    Seong-Lan Yu

    2012-01-01

    Full Text Available Pigs have been considered as donors for xenotransplantation in the replacement of human organs and tissues. However, porcine endogenous retroviruses (PERVs might transmit new infectious disease to humans during xenotransplantation. To investigate PERV integration sites, 45 PERV-positive BAC clones, including 12 PERV-A, 16 PERV-B, and 17 PERV-C clones, were identified from the NIH miniature pig BAC library. The analysis of 12 selected full-length sequences of PERVs, including the long terminal repeat (LTR region, identified the expected of open reading frame length, an indicative of active PERV, in all five PERV-C clones and one of the four PERV-B clones. Premature stop codons were observed in only three PERV-A clones. Also, eleven PERV integration sites were mapped using a 5000-rad IMpRH panel. The map locations of PERV-C clones have not been reported before, thus they are novel PERV clones identified in this study. The results could provide basic information for the elimination of site-specific PERVs in selection of pigs for xenotransplantation.

  19. Construction and characterization of two BAC libraries representing a deep-coverage of the genome of chicory (Cichorium intybus L., Asteraceae

    Directory of Open Access Journals (Sweden)

    Gonthier Lucy

    2010-08-01

    Full Text Available Abstract Background The Asteraceae represents an important plant family with respect to the numbers of species present in the wild and used by man. Nonetheless, genomic resources for Asteraceae species are relatively underdeveloped, hampering within species genetic studies as well as comparative genomics studies at the family level. So far, six BAC libraries have been described for the main crops of the family, i.e. lettuce and sunflower. Here we present the characterization of BAC libraries of chicory (Cichorium intybus L. constructed from two genotypes differing in traits related to sexual and vegetative reproduction. Resolving the molecular mechanisms underlying traits controlling the reproductive system of chicory is a key determinant for hybrid development, and more generally will provide new insights into these traits, which are poorly investigated so far at the molecular level in Asteraceae. Findings Two bacterial artificial chromosome (BAC libraries, CinS2S2 and CinS1S4, were constructed from HindIII-digested high molecular weight DNA of the contrasting genotypes C15 and C30.01, respectively. C15 was hermaphrodite, non-embryogenic, and S2S2 for the S-locus implicated in self-incompatibility, whereas C30.01 was male sterile, embryogenic, and S1S4. The CinS2S2 and CinS1S4 libraries contain 89,088 and 81,408 clones. Mean insert sizes of the CinS2S2 and CinS1S4 clones are 90 and 120 kb, respectively, and provide together a coverage of 12.3 haploid genome equivalents. Contamination with mitochondrial and chloroplast DNA sequences was evaluated with four mitochondrial and four chloroplast specific probes, and was estimated to be 0.024% and 1.00% for the CinS2S2 library, and 0.028% and 2.35% for the CinS1S4 library. Using two single copy genes putatively implicated in somatic embryogenesis, screening of both libraries resulted in detection of 12 and 13 positive clones for each gene, in accordance with expected numbers. Conclusions This

  20. Maintaining Precious Library Resources.

    Science.gov (United States)

    Wiens, Janet

    2002-01-01

    Using the examples of libraries at Southeast Missouri State University and the University of North Texas, discusses the big-picture approach and extensive communication among all users necessary for library maintenance efforts. Addresses establishing a master plan, prioritizing projects, having the right staff, and communicating. (EV)

  1. Construction of a genome-wide human BAC-Unigene resource. Final progress report, 1989--1996

    Energy Technology Data Exchange (ETDEWEB)

    Lim, C.S.; Xu, R.X.; Wang, M. [and others

    1996-12-31

    Currently, over 30,000 mapped STSs and 27,000 mapped Unigenes (non-redundant, unigene sets of cDNA representing EST clusters) are available for human alone. A total of 44,000 Unigene cDNA clones have been supplied by Research Genetics. Unigenes, or cDNAs are excellent resource for map building for two reasons. Firstly, they exist in two alternative forms -- as both sequence information for PCR primer pairs, and cDNA clones -- thus making library screening by colony hybridization as well as pooled library PCR possible. The authors have developed an efficient and robust procedure to screen genomic libraries with large number of DNA probes. Secondly, the linkage and order of expressed sequences, or genes are highly conserved among human, mouse and other mammalian species. Therefore, mapping with cDNA markers rather than random anonymous STSs will greatly facilitate comparative, evolutionary studies as well as physical map building. They have currently deconvoluted over 10,000 Unigene probes against a 4X coverage human BAC clones from the approved library D by high density colony hybridization method. 10,000 batches of Unigenes are arrayed in an imaginary 100 X 100 matrix from which 100 row pools and 100 column pools are obtained. Library filters are hybridized with pooled probes, thus reducing the number of hybridization required for addressing the positives for each Unigene from 10,000 to 200. Details on the experimental scheme as well as daily progress report is posted on the Web site (http://www.tree.caltech.edu).

  2. Repetitive Genomic Elements in a European Corn Borer, Ostrinia nubilalis, BAC Library were Indicated by BAC End Sequencing and Development of Sequence Tag Site Markers: Implications for Lepidopteran Genomic Research

    Science.gov (United States)

    The European corn borer, Ostrinia nubilalis, is a serious pest of food, fiber, and biofuel crops in Europe, North America, and Asia, and a model system for insect olfaction and speciation. A bacterial artificial chromosome (BAC) library constructed for O. nubilalis contains 36,864 clones with estim...

  3. Libraries Serving Handicapped Users Share Resources.

    Science.gov (United States)

    Ames, Jan

    1981-01-01

    Because library resources for the blind and physically handicapped are so scarce, cooperative library activities are needed to insure the best utilization of the limited resources which are available. This article describes several such cooperative efforts. (LLS)

  4. Construction of a bacterial artificial chromosome (BAC) library of Lycopersicon esculentum cv. Stevens and its application to physically map the Sw-5 locus

    NARCIS (Netherlands)

    Spassova, MI; Prins, M; Stevens, MR; Hille, J; Goldbach, RW; Spassova, Mariana I.; Stevens, Mikel R.; Goldbach, Rob W.

    1999-01-01

    The Sw-5 gene is a dominantly inherited resistance gene in tomato and functional against a number of tospovirus species. The gene has been mapped on chromosome 9, tightly linked to RFLP markers CT220 and SCAR421. To analyse the Sw-5 locus, a BAC genomic library was constructed of tomato cv. Stevens,

  5. Electronic Resources in Ohio Prison Libraries.

    Science.gov (United States)

    LaPoint, Virginia A.

    Traditionally, libraries have had a role of providing equitable access for those who might not otherwise have access, and this role continues today in implementing access to electronic resources. However, there are special problems in applying this mission to prison libraries. A short history of automation in prison libraries is followed by a…

  6. Library Resource Categories and Their Possible Groupings

    Science.gov (United States)

    Hider, Philip

    2009-01-01

    The resource typologies proposed in the new standard, "Resource Description and Access" (RDA), are evaluated in the context of a particular university library catalogue through two card-sort exercises. Although it was found that end-users recognised the content and carrier aspects of the resource types as listed in RDA, they did not categorise…

  7. Managing Academic Libraries with Fewer Resources.

    Science.gov (United States)

    Riggs, Donald E.

    1992-01-01

    A discussion of academic library management during retrenchment looks at a variety of issues, including staffing needs in the labor-intensive library environment, acquisitions budgeting, interlibrary cooperation (ownership vs. access to resources), entrepreneurship and strategic planning for problem solving, and use of total quality management…

  8. Construction of an integrated genetic linkage map for the A genome of Brassica napus using SSR markers derived from sequenced BACs in B. rapa

    OpenAIRE

    Xu, Jinsong; Qian, Xiaoju; Wang, Xiaofeng; Li, Ruiyuan; Cheng, Xiaomao; Yang, Yuan; Fu, Jie; Zhang, Shunchang; King, Graham J; Wu, Jiangsheng; Liu, Kede

    2010-01-01

    Background The Multinational Brassica rapa Genome Sequencing Project (BrGSP) has developed valuable genomic resources, including BAC libraries, BAC-end sequences, genetic and physical maps, and seed BAC sequences for Brassica rapa. An integrated linkage map between the amphidiploid B. napus and diploid B. rapa will facilitate the rapid transfer of these valuable resources from B. rapa to B. napus (Oilseed rape, Canola). Results In this study, we identified over 23,000 simple sequence repeats ...

  9. The first insight into the Salvia (Lamiaceae) genome via BAC library construction and high-throughput sequencing of target BAC clones

    OpenAIRE

    Vautrin, Sonia; Song, Chi; Zhu, Ying Jie; Berges, Hélène; Sun, Chao

    2015-01-01

    Salvia is a representative genus of Lamiaceae, a eudicot family with significant species diversity and population adaptibility. One of the key goals of Salvia genomics research is to identify genes of adaptive significance. This information may help to improve the conservation of adaptive genetic variation and the management of medicinal plants to increase their health and productivity. Large-insert genomic libraries are a fundamental tool for achieving this purpose. We report herein the cons...

  10. A five-fold pig bacterial artificial chromosome library:a resource for positional cloning and physical mapping

    Institute of Scientific and Technical Information of China (English)

    LIU Wei; LI Ning; ZHANG Ying; LIU Zhaoliang; GUO Li; WANG Xiaobo; FEI Jing; FENG Jidong; ZHAO Rui; HU Xiaoxiang

    2006-01-01

    A pig BAC library was constructed with genomic DNA from a male Erhualian pig. After partial digestion with Hind Ⅲ or BamH I the fragments obtained were cloned into the pBeloBAC11 vector. The library consists of 184320 clones which stored in 480pieces 384-well plates (20 plates per superpool). A two-step 4-dimension PCR screening system was established to screen the positive clones. An average insert size of 128 kb was estimated from 105 randomly isolated clones, which indicates that the library is more than five times of genomic coverage. For the demonstration of the probability to pick out any unique genes or DNA markers from the library, 10single-copy genes were screened out and the positive clones were yielded between 1 and 8 with an average of 3.6. Positive superpools were obtained for 32 microsatellite markers selected from different regions of pig genome. The number of positive superpools for each marker varies from 1 to 9 with an average of 4.78. This BAC library provides an additional resource for pig physical mapping and gene identification.

  11. Library Support for Resource Constrained Accelerators

    DEFF Research Database (Denmark)

    Brock-Nannestad, Laust; Karlsson, Sven

    2014-01-01

    Accelerators, and other resource constrained systems, are increasingly being used in computer systems. Accelerators provide power efficient performance and often provide a shared memory model. However, it is a challenge to map feature rich APIs, such as OpenMP, to resource constrained systems....... In this paper, we present a lightweight system where an accelerator can remotely execute library functions on a host processor. The implementation takes up 750 bytes but can replace arbitrary library calls leading to significant savings in memory foot print. We evaluate with a set of SPLASH-2 applications...

  12. 应用随机多肽文库分析BAC5单抗相关的抗原表位%Analysis of BAC5 mcAb-Related Epitope Using Random Peptide library

    Institute of Scientific and Technical Information of China (English)

    肖锡宾; 张昌卿; 张颖; 张如华; 李经略; 冯凯涛; 孙韵; 叶永照

    2003-01-01

    Objective To identify epitope relating to BAC5 mcAb, a kind of monoclonal antibody (mcAb) located on the surface of nasopha-ryngeal carcinoma (NPC) cells.Methods Using BAC5 mcAb as a selected target, the 3 rounds of biopanning to a 12 mer random peptide library (RPL) presen-ted by M13 phages were carried out. The positive M13 phage clones were chosen and confirmed with sandwich ELISA for antibodycapture and competitive assay. The exogenous DNA fragments in the positive/negative M13 phages were sequenced to deduce andcompare the order of the amino acids of exogenous peptides among the phage clones.Results 77% (35/45) of the phages eluted from the 3rd round of biopnning could be captured by BAC5 mcAb. The 3 kinds ofthe peptides were displayed by M13 phages from the 8 positive clones identified with competitive assay. The same character of "-P-V-"structure existed near N-terminus of the 3 different peptides, i.e. -H-Q-S-H-Y-P-Y-P-V-V-S-L- (4/8) -Q-N-Q-A-W-F-S-Q-P-V-R-M- (3/8) and T-Q-A-Y-K-G-F-P-V-L-P-S- (1/8) in comparison with the peptide" -N-H-Q-S-T-F-W-Q-K-W-T-A-" dis-played by M13 phages from the negative clones (6/6).Conclusion BAC5 mcAb can recognize the 3 kinds of the peptides with-P-V-structure near N-terminus. These peptides mimic thestructure of the epitope on the surface of NPC cells recognized by BAC5 mcAb.%目的 鉴定能被BAC5单抗识别的定位于鼻咽癌细胞表面的抗原表位.方法 应用BAC5单抗作为靶抗体对噬菌体呈现的随机12肽文库进行3轮生物淘洗,用抗体捕获和竞争试验的夹心ELISA方法选择和鉴定阳性噬菌体克隆,对阳性和阴性噬菌体的外源性DNA片段进行序列分析,推导和比较由这些噬菌体所呈现的多肽氨基酸序列.结果 通过3轮生物淘洗能被抗体捕获的噬菌体克隆为77%(35/45).用竞争试验从所捕获的克隆中测得8个阳性克隆.来自这8个克隆的噬菌体呈现三种外源多肽,即-H-Q-S-H-Y-P-Y-P-V-V-S-L-(4/8)-Q-N-Q-A

  13. Identification of an extensive gene cluster among a family of PPOs in Trifolium pratense L. (red clover using a large insert BAC library

    Directory of Open Access Journals (Sweden)

    Thomas Ann

    2009-07-01

    Full Text Available Abstract Background Polyphenol oxidase (PPO activity in plants is a trait with potential economic, agricultural and environmental impact. In relation to the food industry, PPO-induced browning causes unacceptable discolouration in fruit and vegetables: from an agriculture perspective, PPO can protect plants against pathogens and environmental stress, improve ruminant growth by increasing nitrogen absorption and decreasing nitrogen loss to the environment through the animal's urine. The high PPO legume, red clover, has a significant economic and environmental role in sustaining low-input organic and conventional farms. Molecular markers for a range of important agricultural traits are being developed for red clover and improved knowledge of PPO genes and their structure will facilitate molecular breeding. Results A bacterial artificial chromosome (BAC library comprising 26,016 BAC clones with an average 135 Kb insert size, was constructed from Trifolium pratense L. (red clover, a diploid legume with a haploid genome size of 440–637 Mb. Library coverage of 6–8 genome equivalents ensured good representation of genes: the library was screened for polyphenol oxidase (PPO genes. Two single copy PPO genes, PPO4 and PPO5, were identified to add to a family of three, previously reported, paralogous genes (PPO1–PPO3. Multiple PPO1 copies were identified and characterised revealing a subfamily comprising three variants PPO1/2, PPO1/4 and PPO1/5. Six PPO genes clustered within the genome: four separate BAC clones could be assembled onto a predicted 190–510 Kb single BAC contig. Conclusion A PPO gene family in red clover resides as a cluster of at least 6 genes. Three of these genes have high homology, suggesting a more recent evolutionary event. This PPO cluster covers a longer region of the genome than clusters detected in rice or previously reported in tomato. Full-length coding sequences from PPO4, PPO5, PPO1/5 and PPO1/4 will facilitate

  14. Construction of an integrated genetic linkage map for the A genome of Brassica napus using SSR markers derived from sequenced BACs in B. rapa

    OpenAIRE

    King Graham J; Zhang Shunchang; Fu Jie, .; Yang Yuan; Cheng Xiaomao; Li Ruiyuan; Wang Xiaofeng; Qian Xiaoju; Xu Jinsong; Wu Jiangsheng; Liu Kede

    2010-01-01

    Abstract Background The Multinational Brassica rapa Genome Sequencing Project (BrGSP) has developed valuable genomic resources, including BAC libraries, BAC-end sequences, genetic and physical maps, and seed BAC sequences for Brassica rapa. An integrated linkage map between the amphidiploid B. napus and diploid B. rapa will facilitate the rapid transfer of these valuable resources from B. rapa to B. napus (Oilseed rape, Canola). Results In this study, we identified over 23,000 simple sequence...

  15. Use of electronic information resources in goverment libraries

    OpenAIRE

    Simona Omahen; Maja Žumer

    2003-01-01

    The article presents the use of electronic information resources in government libraries in Slovenia. It starts with the definition of government libraries and electronic publications. On a selected sample of government libraries, the state of the usage of electronic information resources in government libraries was studied. On the basis of interviews, carried out in five government libraries, it was established that government libraries mostly do not focus on, or even think about, the use of...

  16. Library Resources and Services and Publication Productivity

    OpenAIRE

    Ngah, Z.A.

    2001-01-01

    Present the results of a study that compares the perceived adequacy of library resources for research, the formal channels found to be useful in providing information needed for research, the methods used to keep abreast with current research literature, the problems faced when obtaining information required for research with publication productivity of 83 academic engineers and 239 academic scientists from the University of Malaya and National University of Malaysia. Respondents' views were ...

  17. Integrating print and digital resources in library collections

    CERN Document Server

    Katz, Linda S

    2013-01-01

    Get informed answers to your questions and concerns about integrating the materials in your library's collectionLibrary collections have always included materials in many formats?handling a mix of material types is an accepted part of library work. And in recent years, the very concept of ?collection? has been significantly redefined by the addition of electronic resources. But are print and digital materials really merged in library collections or are they treated and maintained as separate entities? Integrating Print and Digital Resources in Library Collections examines a variety

  18. Integrating Electronic Resources into the Library Catalog: A Collaborative Approach.

    Science.gov (United States)

    Herrera, Gail; Aldana, Lynda

    2001-01-01

    Describes a project at the University of Mississippi Libraries to catalog purchased electronic resources so that access to these resources is available only via the Web-based library catalog. Discusses collaboration between cataloging and systems personnel; and describes the MARC catalog record field that contains the information needed to locate…

  19. Library Resources and Services for Faculty.

    Science.gov (United States)

    Bonn, Robert; And Others

    This pamphlet was designed to acquaint faculty at the John Jay College of Criminal Justice with general non-criminal justice services available in the college library for their own research and study. Emphasis is placed on using the reference collection of a small library and the expertise of the library staff to identify and locate information…

  20. Screening of tissues pooled cDNA library using probes by restricted fragments of BAC positive clones of ovine MHC%以绵羊MHC 区段BAC 克隆酶切片段为探针杂交筛选绵羊混合组织cDNA 文库

    Institute of Scientific and Technical Information of China (English)

    杨小亮; 白大章; 邱巍; 董慧芹; 李大全; 陈芳; 马润林; Hugh T Blair; 高剑峰

    2012-01-01

    Under the premise what we have known bacterial artificial chromosome(BAC) clone sequence information and gene annotation predicted in the Chinese Merino sheep major histocompatibility complex (MHC) region, the digested fragments from 6 BAC clones that were located in the MHC region of the Chinese Merino sheep genome BAC library, which were used to screen the cDNA library using plaque in situ hybridization as probes. The full length of positive cDNA clones (sequences) isolated were completely sequenced, and the sequences obtained were aligned with the corresponding known sequence information and the BAC clones with gene annotation. Meanwhile, the sequence similarity was searched in NCBI Blastn database. This work aimed at verification of accuracy of the gene annotation results and initial analysis of gene (sequence) function. At last, 27 positive cDNA clones (sequences) in total were screened through two runs of hybridization. It was also found that these sequences could be positioned in the corresponding BAC clones, and 25 sequences were located in exon area of the annotated gene. It was verified that 23 sequences had the highest sequence similarity with those in the Bos taurus by searching against the NCBI Blastn database; moreover, the function of these sequences were closely relate to immunology.%在已知中国美利奴羊MHC(Major histocompatibility complex)区段BAC(Bacterial artificial chromosome)克隆序列信息和预测的基因注释前提下,用位于中国美利奴羊基因组BAC 文库MHC 区段的6 个BAC 克隆酶切片段为探针,以噬菌斑原位杂交筛选法筛选中国美利奴羊混合组织cDNA 文库(库库杂交),对分离到的cDNA阳性克隆进行全序列测定,并与相应的已知序列信息和基因注释的BAC 克隆比对以及在NCBI Blastn 数据库中序列相似性检索,旨在验证基因注释结果的准确性和对基因(序列)功能的初步分析.实验中,经过两轮杂交共筛选出27 个cDNA 阳性克隆(序

  1. FILTR: Flash Isotope Library and Training Resource

    International Nuclear Information System (INIS)

    The subject of radiation detection is replete with complex concepts and challenging nomenclature. Furthermore, a daunting variety of radioactive isotopes may be encountered during the routine operation of a radiation detector. Individuals tasked with searching for illicit sources of radiation must remain vigilant while navigating through more frequently encountered mundane and legitimate radioactive sources. The Flash Isotope Library and Training Resource (FILTR) is being developed as an easily accessible and intuitive reference tool to manage the high volume of complex information required for this task. FILTR is an extended version of the Primary Utility for Nuclear Terminology (PUNT) software developed by the Counter Measures Test Beds group at Lawrence Livermore National Laboratory for the United States Secret Service. Authored in the Flash multimedia development environment, FILTR contains detailed information on potentially encountered isotopes as well as training on radiation and operational procedures. Reference material is organized to present critical information quickly while facilitating more in-depth investigation through an intuitive interface and engaging content. FILTR is being developed for a diverse audience of law enforcement organizations and government agencies and a wide range of skill sets from expert analysts to officers whose primary role is not radiation detection. Additionally, the wide compatibility of Flash content will allow FILTR to be readily accessible through the growing number of multi-media enabled electronic devices, including PDAs and cellular phones

  2. Utilization of Super BAC Pools and Fluidigm Access Array Platform for High-Throughput BAC Clone Identification: Proof of Concept

    OpenAIRE

    Peter J Maughan; Smith, Scott M.; Joshua A. Raney

    2012-01-01

    Bacterial artificial chromosome (BAC) libraries are critical for identifying full-length genomic sequences, correlating genetic and physical maps, and comparative genomics. Here we describe the utilization of the Fluidigm access array genotyping system in conjunction with KASPar genotyping technology to identify individual BAC clones corresponding to specific single-nucleotide polymorphisms (SNPs) from an Amplicon Express seven-plate super pooled Amaranthus hypochondriacus BAC library. Ninety...

  3. Multi-registration of software library resources

    Science.gov (United States)

    Archer, Charles J.; Blocksome, Michael A.; Ratterman, Joseph D.; Smith, Brian E.

    2011-04-05

    Data communications, including issuing, by an application program to a high level data communications library, a request for initialization of a data communications service; issuing to a low level data communications library a request for registration of data communications functions; registering the data communications functions, including instantiating a factory object for each of the one or more data communications functions; issuing by the application program an instruction to execute a designated data communications function; issuing, to the low level data communications library, an instruction to execute the designated data communications function, including passing to the low level data communications library a call parameter that identifies a factory object; creating with the identified factory object the data communications object that implements the data communications function according to the protocol; and executing by the low level data communications library the designated data communications function.

  4. Resource sharing in libraries concepts, products, technologies, and trends

    CERN Document Server

    Breeding, Marshall

    2014-01-01

    Supplementing your local collection through resource sharing is a smart way to ensure your library has the resources to satisfy the needs of your users. Marshall Breeding's new Library Technology Report explores technologies and strategies for sharing resources, helping you streamline workflows and improve resource-sharing services by covering key strategies like interlibrary loan, consortial borrowing, document delivery, and shared collections. You'll also learn about such trends and services as:OCLC WorldCat Resource Sharing, and other systems that facilitate cooperative, reciprocal lendingS

  5. Performing by the Book: Library Resources for Theatre Artists.

    Science.gov (United States)

    Kahn, David

    1994-01-01

    Discussion of the role of library resources in the formulation of theatrical performance features the dramaturg, the professional researcher on a theater's staff. Topics include an empirical approach to performance; how theater artists use libraries; the dramaturg as model; researching the text; dramaturgical case studies; and relationships…

  6. Library Programs: Interlibrary Cooperation and Resource Sharing. Fiscal Year 1987.

    Science.gov (United States)

    Kittel, Dorothy

    This overview of how the 50 states used their Library Services and Construction Act funds under Title III (Interlibrary Cooperation and Resource Sharing) in fiscal year 1987 reports that, overall, state library agencies spent $16 million in Title III funds to work toward the creation of statewide databases of the bibliographic holdings of all…

  7. Resource Provisions of a High School Library Collection

    Science.gov (United States)

    Collins, Karla B.; Doll, Carol A.

    2012-01-01

    The mission of the school library "is to ensure students and staff are effective users of ideas and information" (AASL 2009, 8). The school library collection should, therefore, support instruction throughout the school. However, teachers do not always understand the potential value of the resources available. This research explored perceptions…

  8. American Indian Resource Manual for Public Libraries. Bulletin No. 92429.

    Science.gov (United States)

    de Usabel, Frances; Roeber, Jane A.

    This manual, written primarily for Wisconsin public librarians, is designed to provide practical assistance to those who wish to expand public library collections of American Indian (defined as people in the United States and Canada who trace their lineage to the original habitants of these lands) resources and to improve related library programs…

  9. National Library of Medicine Web Resources for Student Health Professionals

    Energy Technology Data Exchange (ETDEWEB)

    Womble, R.

    2010-04-02

    Familiarize students affiliated with the Student National Medical Association with the National Library of Medicine's online resources that address medical conditions, health disparities, and public health preparedness needs.

  10. Distribution of Genes and Repetitive Elements in the Diabrotica virgifera virgifera Genome Estimated Using BAC Sequencing

    Directory of Open Access Journals (Sweden)

    Brad S. Coates

    2012-01-01

    Full Text Available Feeding damage caused by the western corn rootworm, Diabrotica virgifera virgifera, is destructive to corn plants in North America and Europe where control remains challenging due to evolution of resistance to chemical and transgenic toxins. A BAC library, DvvBAC1, containing 109,486 clones with 104±34.5 kb inserts was created, which has an ~4.56X genome coverage based upon a 2.58 Gb (2.80 pg flow cytometry-estimated haploid genome size. Paired end sequencing of 1037 BAC inserts produced 1.17 Mb of data (~0.05% genome coverage and indicated ~9.4 and 16.0% of reads encode, respectively, endogenous genes and transposable elements (TEs. Sequencing genes within BAC full inserts demonstrated that TE densities are high within intergenic and intron regions and contribute to the increased gene size. Comparison of homologous genome regions cloned within different BAC clones indicated that TE movement may cause haplotype variation within the inbred strain. The data presented here indicate that the D. virgifera virgifera genome is large in size and contains a high proportion of repetitive sequence. These BAC sequencing methods that are applicable for characterization of genomes prior to sequencing may likely be valuable resources for genome annotation as well as scaffolding.

  11. Resource discovery in distributed digital libraries through visual knowledge navigation

    Institute of Scientific and Technical Information of China (English)

    GU Qian-yi; AHMAD Faisal; SUMNER Tamara

    2005-01-01

    In order to support users to search and browse for various resources, digital libraries are composed of discovery systems which provide user interface and information retrieve system. Recent researches in Information Retrieval have investigated different techniques through improving precision and recall to enhance the effectiveness of discovery system in digital libraries. In this paper, we present our work to enhance discovery system effectiveness with a different approach, through resource discovery based on visual knowledge navigation. In our Strand Map Services project under National Science Digital Library, we introduce the visual resource discovery system called conceptual browsing interfaces, to help educators and learners to locate,comprehend and use educational resources in digital libraries. The paper begins with a short introduction of the Strand Map Services. Then we illustrate the service architecture, the design and implementation of its major components. We will focus our discussion of how the visualization system of the Strand Map Services supports the visual knowledge navigation for distributed digital libraries. This includes the knowledge acquisition of the conceptual browsing interfaces, different knowledge representations in the system perspective and user interface perspective, visualization system modules, algorithm and Web services integration to use visual knowledge navigation to enhance resource discovery in digital libraries.

  12. Graphic Resources in the Spanish Art Libraries.

    Science.gov (United States)

    Medina, Alicia Garcia; Coso, Teresa

    The interest in graphic information has increased explosively and is explored through various communication networks like the Internet and the audiovisual media. Art libraries, museums, cultural centers, and art foundations manage and store a lot of images in several departments and in print and digital formats. This paper discusses the cataloging…

  13. Why Should I Use University Library Website Resources? Discipline Differences

    Science.gov (United States)

    Kim, Yong-Mi

    2011-01-01

    Users across academic disciplines utilize different information sources based on the resource's usefulness and relevance. This study's findings show that users from arts and sciences disciplines are much more likely to utilize university library website resources and printed materials than business users who heavily rely on commercial websites.…

  14. The National Resource Library for Science and Technology in Sweden: A Nordic model of cooperating technology libraries

    Science.gov (United States)

    Lagar, Gunnar

    1994-01-01

    The scope of this presentation is to give a state-of-the-art report on the present situation of Nordic technology libraries, to elaborate on a plan for national resource libraries in Sweden, and to share how the Royal Institute of Technology Library in Stockholm (KTHB) has fostered a network of cooperating libraries in order to optimize government funding for the system of resource libraries.

  15. Construction of an integrated genetic linkage map for the A genome of Brassica napus using SSR markers derived from sequenced BACs in B. rapa

    Directory of Open Access Journals (Sweden)

    King Graham J

    2010-10-01

    Full Text Available Abstract Background The Multinational Brassica rapa Genome Sequencing Project (BrGSP has developed valuable genomic resources, including BAC libraries, BAC-end sequences, genetic and physical maps, and seed BAC sequences for Brassica rapa. An integrated linkage map between the amphidiploid B. napus and diploid B. rapa will facilitate the rapid transfer of these valuable resources from B. rapa to B. napus (Oilseed rape, Canola. Results In this study, we identified over 23,000 simple sequence repeats (SSRs from 536 sequenced BACs. 890 SSR markers (designated as BrGMS were developed and used for the construction of an integrated linkage map for the A genome in B. rapa and B. napus. Two hundred and nineteen BrGMS markers were integrated to an existing B. napus linkage map (BnaNZDH. Among these mapped BrGMS markers, 168 were only distributed on the A genome linkage groups (LGs, 18 distrubuted both on the A and C genome LGs, and 33 only distributed on the C genome LGs. Most of the A genome LGs in B. napus were collinear with the homoeologous LGs in B. rapa, although minor inversions or rearrangements occurred on A2 and A9. The mapping of these BAC-specific SSR markers enabled assignment of 161 sequenced B. rapa BACs, as well as the associated BAC contigs to the A genome LGs of B. napus. Conclusion The genetic mapping of SSR markers derived from sequenced BACs in B. rapa enabled direct links to be established between the B. napus linkage map and a B. rapa physical map, and thus the assignment of B. rapa BACs and the associated BAC contigs to the B. napus linkage map. This integrated genetic linkage map will facilitate exploitation of the B. rapa annotated genomic resources for gene tagging and map-based cloning in B. napus, and for comparative analysis of the A genome within Brassica species.

  16. Euler European Libraries and Electronic Resources in Mathematical Sciences

    CERN Document Server

    The Euler Project. Karlsruhe

    The European Libraries and Electronic Resources (EULER) Project in Mathematical Sciences provides the EulerService site for searching out "mathematical resources such as books, pre-prints, web-pages, abstracts, proceedings, serials, technical reports preprints) and NetLab (for Internet resources), this outstanding engine is capable of simple, full, and refined searches. It also offers a browse option, which responds to entries in the author, keyword, and title fields. Further information about the Project is provided at the EULER homepage.

  17. Optimal Resource Allocation in Library Systems

    Science.gov (United States)

    Rouse, William B.

    1975-01-01

    Queueing theory is used to model processes as either waiting or balking processes. The optimal allocation of resources to these processes is defined as that which maximizes the expected value of the decision-maker's utility function. (Author)

  18. A BAC-based physical map of the Drosophila buzzatii genome

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez, Josefa; Nefedov, Michael; Bosdet, Ian; Casals, Ferran; Calvete, Oriol; Delprat, Alejandra; Shin, Heesun; Chiu, Readman; Mathewson, Carrie; Wye, Natasja; Hoskins, Roger A.; Schein, JacquelineE.; de Jong, Pieter; Ruiz, Alfredo

    2005-03-18

    Large-insert genomic libraries facilitate cloning of large genomic regions, allow the construction of clone-based physical maps and provide useful resources for sequencing entire genomes. Drosophilabuzzatii is a representative species of the repleta group in the Drosophila subgenus, which is being widely used as a model in studies of genome evolution, ecological adaptation and speciation. We constructed a Bacterial Artificial Chromosome (BAC) genomic library of D. buzzatii using the shuttle vector pTARBAC2.1. The library comprises 18,353 clones with an average insert size of 152 kb and a {approx}18X expected representation of the D. buzzatii euchromatic genome. We screened the entire library with six euchromatic gene probes and estimated the actual genome representation to be {approx}23X. In addition, we fingerprinted by restriction digestion and agarose gel electrophoresis a sample of 9,555 clones, and assembled them using Finger Printed Contigs (FPC) software and manual editing into 345 contigs (mean of 26 clones per contig) and 670singletons. Finally, we anchored 181 large contigs (containing 7,788clones) to the D. buzzatii salivary gland polytene chromosomes by in situ hybridization of 427 representative clones. The BAC library and a database with all the information regarding the high coverage BAC-based physical map described in this paper are available to the research community.

  19. Construction and Analysis of Siberian Tiger Bacterial Artificial Chromosome Library with Approximately 6.5-Fold Genome Equivalent Coverage

    Directory of Open Access Journals (Sweden)

    Changqing Liu

    2014-03-01

    Full Text Available Bacterial artificial chromosome (BAC libraries are extremely valuable for the genome-wide genetic dissection of complex organisms. The Siberian tiger, one of the most well-known wild primitive carnivores in China, is an endangered animal. In order to promote research on its genome, a high-redundancy BAC library of the Siberian tiger was constructed and characterized. The library is divided into two sub-libraries prepared from blood cells and two sub-libraries prepared from fibroblasts. This BAC library contains 153,600 individually archived clones; for PCR-based screening of the library, BACs were placed into 40 superpools of 10 × 384-deep well microplates. The average insert size of BAC clones was estimated to be 116.5 kb, representing approximately 6.46 genome equivalents of the haploid genome and affording a 98.86% statistical probability of obtaining at least one clone containing a unique DNA sequence. Screening the library with 19 microsatellite markers and a SRY sequence revealed that each of these markers were present in the library; the average number of positive clones per marker was 6.74 (range 2 to 12, consistent with 6.46 coverage of the tiger genome. Additionally, we identified 72 microsatellite markers that could potentially be used as genetic markers. This BAC library will serve as a valuable resource for physical mapping, comparative genomic study and large-scale genome sequencing in the tiger.

  20. Micronesian Art Historical Research and Library Collection Resources in Micronesia.

    Science.gov (United States)

    Haynes, Douglas

    This paper briefly describes the geographic region and some indigenous artifacts of Micronesia. The state of art historical research in the area and currently available library resources are discussed. Micronesia is comprised of seven island nations peopled by distinctly unique cultural groups. Study of Micronesian art and architecture is…

  1. School Library Journal's 10 Best Digital Resources for 2009

    Science.gov (United States)

    Brisco, Shonda

    2009-01-01

    The author presents 10 best digital resources for 2009. As librarians prepare for the next school year--or as public libraries develop the budget for a new fiscal year--these are the products for children and teens that should be advocated to add to one's digital collection. These include: (1) American Indian Experience; (2) Animoto…

  2. Shaping the Curriculum: The Power of a Library's Digital Resources

    Science.gov (United States)

    Kirkwood, Patricia

    2011-01-01

    Researchers were the first adopters of digital resources available through the library. Online journals and databases make finding research articles much easier than when this author started as a librarian more than 20 years ago. Speedier interlibrary loan due to digital delivery means research materials are never far away. Making it easier for…

  3. Sequencing,annotation and comparative analysis of nine BACs of the giant panda(Ailuropoda melanoleuca)

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    A 10-fold BAC library for the giant panda was constructed and nine BACs were selected to generate finish sequences.These BACs could be used as a validation resource for the de novo assembly accuracy of the whole genome shotgun sequencing reads of the giant panda newly generated by Illumina GA sequencing technology.Complete Sanger sequencing,assembly,annotation and comparative analysis were carried out on the selected BACs of a joint length 878 kb.Homologue search and de novo prediction methods were used to annotate genes and repeats.Twelve protein coding genes were predicted,seven of which could be functionally annotated.The seven genes have an average gene size of about 41 kb,an average coding size of about 1.2 kb and an average exon number of 6 per gene.Besides,seven tRNA genes were found.About 27 percent of the BAC sequence is composed of repeats.A phylogenetic tree was constructed using a neighbor-join algorithm across five species,including the giant panda,human,dog,cat and mouse,which reconfirms dog as the most closely related species to the giant panda.Our results provide detailed sequence and structure information for new genes and repeats of the giant panda,which will be helpful for further studies about the giant panda.

  4. Development of genomic resources for Citrus clementina: Characterization of three deep-coverage BAC libraries and analysis of 46,000 BAC end sequences

    OpenAIRE

    Talon Manuel; Ollitrault Patrick; Naranjo M Angel; Terol Javier

    2008-01-01

    Abstract Background Citrus species constitute one of the major tree fruit crops of the subtropical regions with great economic importance. However, their peculiar reproductive characteristics, low genetic diversity and the long-term nature of tree breeding mostly impair citrus variety improvement. In woody plants, genomic science holds promise of improvements and in the Citrus genera the development of genomic tools may be crucial for further crop improvements. In this work we report the char...

  5. Human resource management and career planning in a larger library

    Directory of Open Access Journals (Sweden)

    Jelka Gazvoda

    1997-01-01

    Full Text Available Human resource management is presented as a managerial function which is used to develop potential abilities of the employees to achieve organizational goals.Different perception of the employees is essential - people working in the organization are treated as capital and not as an expenditure. In human resource management the most important view of the employees is their potential growth and professional development, training for acquiring new responsibilities and encouragement for innovation. Library management is becoming more and more complex as the result of introducing new technologies. For this reason libraries need well trained people with potentials to modernize library performance and to overcome the conflict between the traditional organizational culture and the requirements of the modem technologically developed environment. The author presents different techniques of active human resource management, which can be used in larger libraries where an appropriate number of employees exists to realize different programmes with. These are programmes for education, staffing,career planning, stimmulation and reward systems, job redefinition and enrichment,and other forms of internal segmentation.

  6. Toward a Molecular Cytogenetic Map for Cultivated Sunflower (Helianthus annuus L.) by Landed BAC/BIBAC Clones

    Science.gov (United States)

    Feng, Jiuhuan; Liu, Zhao; Cai, Xiwen; Jan, Chao-Chien

    2013-01-01

    Conventional karyotypes and various genetic linkage maps have been established in sunflower (Helianthus annuus L., 2n = 34). However, the relationship between linkage groups and individual chromosomes of sunflower remains unknown and has considerable relevance for the sunflower research community. Recently, a set of linkage group-specific bacterial /binary bacterial artificial chromosome (BAC/BIBAC) clones was identified from two complementary BAC and BIBAC libraries constructed for cultivated sunflower cv. HA89. In the present study, we used these linkage group-specific clones (∼100 kb in size) as probes to in situ hybridize to HA89 mitotic chromosomes at metaphase using the BAC- fluorescence in situ hybridization (FISH) technique. Because a characteristic of the sunflower genome is the abundance of repetitive DNA sequences, a high ratio of blocking DNA to probe DNA was applied to hybridization reactions to minimize the background noise. As a result, all sunflower chromosomes were anchored by one or two BAC/BIBAC clones with specific FISH signals. FISH analysis based on tandem repetitive sequences, such as rRNA genes, has been previously reported; however, the BAC-FISH technique developed here using restriction fragment length polymorphism (RFLP)−derived BAC/BIBAC clones as probes to apply genome-wide analysis is new for sunflower. As chromosome-specific cytogenetic markers, the selected BAC/BIBAC clones that encompass the 17 linkage groups provide a valuable tool for identifying sunflower cytogenetic stocks (such as trisomics) and tracking alien chromosomes in interspecific crosses. This work also demonstrates the potential of using a large-insert DNA library for the development of molecular cytogenetic resources. PMID:23316437

  7. Usability Testing for e-Resource Discovery: How Students Find and Choose e-Resources Using Library Web Sites

    Science.gov (United States)

    Fry, Amy; Rich, Linda

    2011-01-01

    In early 2010, library staff at Bowling Green State University (BGSU) in Ohio designed and conducted a usability study of key parts of the library web site, focusing on the web pages generated by the library's electronic resources management system (ERM) that list and describe the library's databases. The goal was to discover how users find and…

  8. Construction and Preliminary Characterization Analysis of Wuzhishan Miniature Pig Bacterial Artificial Chromosome Library with Approximately 8-Fold Genome Equivalent Coverage

    Directory of Open Access Journals (Sweden)

    Changqing Liu

    2013-01-01

    Full Text Available Bacterial artificial chromosome (BAC libraries have been invaluable tools for the genome-wide genetic dissection of complex organisms. Here, we report the construction and characterization of a high-redundancy BAC library from a very valuable pig breed in China, Wuzhishan miniature pig (Sus scrofa, using its blood cells and fibroblasts, respectively. The library contains approximately 153,600 clones ordered in 40 superpools of 10 × 384-deep well microplates. The average insert size of BAC clones was estimated to be 152.3 kb, representing approximately 7.68 genome equivalents of the porcine haploid genome and a 99.93% statistical probability of obtaining at least one clone containing a unique DNA sequence in the library. 19 pairs of microsatellite marker primers covering porcine chromosomes were used for screening the BAC library, which showed that each of these markers was positive in the library; the positive clone number was 2 to 9, and the average number was 7.89, which was consistent with 7.68-fold coverage of the porcine genome. And there were no significant differences of genomic BAC library from blood cells and fibroblast cells. Therefore, we identified 19 microsatellite markers that could potentially be used as genetic markers. As a result, this BAC library will serve as a valuable resource for gene identification, physical mapping, and comparative genomics and large-scale genome sequencing in the porcine.

  9. RESOURCES CONSUMPTION MANAGEMENT AT THE NATIONAL LIBRARY OF IRAN

    Directory of Open Access Journals (Sweden)

    K. Naddafi, J. Nouri, R. Nabizadeh, N. Shahbod

    2007-07-01

    Full Text Available This paper studies the various resources (water, energy, paper as well as other wastes consumption management at the National Library of Iran in Tehran. After setting the targets and specifying the correct management framework of resources consumption through the green management schedule, the resources analysis in consumption pattern in National Library of Iran was set up. Some attempt plans were performed to find out management solutions to the related issues. For the achievement of this aim, the first step was considered the study of general conditions of the Library through several visits, completing the checklists and questionnaires and then gathering the necessary information and analyzing them. The results of the fuel consumption in transportations related to the National Library showed that the major pollutants produced by the transportation were CO2, CO, NOx, and hydrocarbons. The rate of CO2 had a maximum level of 904 Kg and CO was 6 Kg, NOx was 2 Kg and hydrocarbons were 0.58 Kg. Among the equipments, which use energy in the Library, refrigerators produce the levels of CO2, SO2 and NOx .The total level of CO2, SO2 and NOx produced by all the equipments were 1140.32 Kg, 3.9072 kg and 2.886 Kg respectively. The results of water investigations showed that the total water consumption was 45459 L/year and the greater part of it was related to irrigation of the garden in the courtyard which was equal to 32471 L/year. Regarding to the wastes, the results of 30 times sampling showed the highest level to be related to aluminum and paper and a computer analysis revealed these results in the rates of 19745 Kg/year of CO2, 77.54 Kg/year, SO2 57.4Kg/year NOx and particulate matter was 2.92 Kg/year. Following the investigations carried out in management, executive solutions were suggested which led to the conclusion that the Green Management System should be established at this organization which should result in optimum consumption of resources

  10. 牦牛瘤胃元基因组文库中木聚糖酶基因的分析%Analysis of xylanases derived from the metagenomic BAC clone library of yak rumen

    Institute of Scientific and Technical Information of China (English)

    王敏; 陈富荣; 张山; 朱雅新; 东秀珠; 黄力; 田瑞华; 董志扬; 戴欣

    2011-01-01

    [ Objective ] The diversity of rumen microbial xylanases and their degradation characteristics were studied and the resources of new xylanases genes and enzymes were provided. [ Methods ] According to the result of the high-throughput sequencing of the rumen microbial metagenome bacterial artificial chromosome ( B AC ) clones library, the diversity of xylanase genes was analyzed and screened. Then one xylanase and its downstream xylosidase gene screened was cloned and expressed in Escherichia coli. The enzyme characterization of the recombinant xylanase and xylosidase and their synergistic effect were studied. [Results] All 14 xylanases screened from the library belong to GH10 family proteins. These xylanases shared the amino acid sequence similarity between 20. 5% and 91. 3% . Intriguingly, 7 xylanase genes in different contigs were found to be followed by xylosidase gene. The specific enzyme activity of the xylanase (Xyn32) was 1.98 U/mg and no ferulic acid esterase activity was detected. The specific enzyme activity of the coupled xylosidase (Xyl33) was 0.07 IU/mg, and xylosidase ( Xyl33 ) also displayed the activity of arabinofuranosidase. In addition, the in vitro experiment confirmed the synergistic effect between the coupled xylanase and xylosidase.%[目的]了解牦牛瘤胃微生物木聚糖酶多样性及其降解特征,为木聚糖降解提供新的基因资源.[方法]根据对已构建的瘤胃微生物元基因组细菌人工染色体( BAC)克隆文库高通量测序结果的注释,筛选其中编码木聚糖酶的基因并进行多样性分析;对其中一个木聚糖酶基因及其连锁的木糖苷酶基因进行克隆表达和酶学性质表征,分析其协同作用.[结果]共筛选到14个木聚糖酶基因,均编码GH1O家族木聚糖酶,其氨基酸序列之间的相似性为20.5% -91.3%;其中7个木聚糖酶基因所在的不同的DNA片段(contig)上存在木糖苷酶基因,编码的木糖苷酶属于GH43或GH3糖苷水解酶家

  11. AWARENESS AND USE OF DIGITAL RESOURCES AND SERVICES IN THE IIT DELHI LIBRARY

    OpenAIRE

    Dr. Javed Khan

    2016-01-01

    Information technology (IT) has thrown a new challenge to the libraries. The technology has shown a great impact on the services of the libraries. Libraries use IT for better services and satisfying diverse user needs. Libraries have transformed into digital and virtual libraries where books, journals, and magazines have changed into e-books, e-journals, and e-zines. This has increased the global dissemination of information. Electronic resources (e-resources) are easily accessible in the rem...

  12. A BAC-based physical map of the Hessian fly genome anchored to polytene chromosomes

    Directory of Open Access Journals (Sweden)

    Fellers John P

    2009-07-01

    Full Text Available Abstract Background The Hessian fly (Mayetiola destructor is an important insect pest of wheat. It has tractable genetics, polytene chromosomes, and a small genome (158 Mb. Investigation of the Hessian fly presents excellent opportunities to study plant-insect interactions and the molecular mechanisms underlying genome imprinting and chromosome elimination. A physical map is needed to improve the ability to perform both positional cloning and comparative genomic analyses with the fully sequenced genomes of other dipteran species. Results An FPC-based genome wide physical map of the Hessian fly was constructed and anchored to the insect's polytene chromosomes. Bacterial artificial chromosome (BAC clones corresponding to 12-fold coverage of the Hessian fly genome were fingerprinted, using high information content fingerprinting (HIFC methodology, and end-sequenced. Fluorescence in situ hybridization (FISH co-localized two BAC clones from each of the 196 longest contigs on the polytene chromosomes. An additional 70 contigs were positioned using a single FISH probe. The 266 FISH mapped contigs were evenly distributed and covered 60% of the genome (95,668 kb. The ends of the fingerprinted BACs were then sequenced to develop the capacity to create sequenced tagged site (STS markers on the BACs in the map. Only 3.64% of the BAC-end sequence was composed of transposable elements, helicases, ribosomal repeats, simple sequence repeats, and sequences of low complexity. A relatively large fraction (14.27% of the BES was comprised of multi-copy gene sequences. Nearly 1% of the end sequence was composed of simple sequence repeats (SSRs. Conclusion This physical map provides the foundation for high-resolution genetic mapping, map-based cloning, and assembly of complete genome sequencing data. The results indicate that restriction fragment length heterogeneity in BAC libraries used to construct physical maps lower the length and the depth of the contigs, but is

  13. Genomic tools development for Aquilegia: construction of a BAC-based physical map

    Directory of Open Access Journals (Sweden)

    Hodges Scott A

    2010-11-01

    Full Text Available Abstract Background The genus Aquilegia, consisting of approximately 70 taxa, is a member of the basal eudicot lineage, Ranuculales, which is evolutionarily intermediate between monocots and core eudicots, and represents a relatively unstudied clade in the angiosperm phylogenetic tree that bridges the gap between these two major plant groups. Aquilegia species are closely related and their distribution covers highly diverse habitats. These provide rich resources to better understand the genetic basis of adaptation to different pollinators and habitats that in turn leads to rapid speciation. To gain insights into the genome structure and facilitate gene identification, comparative genomics and whole-genome shotgun sequencing assembly, BAC-based genomics resources are of crucial importance. Results BAC-based genomic resources, including two BAC libraries, a physical map with anchored markers and BAC end sequences, were established from A. formosa. The physical map was composed of a total of 50,155 BAC clones in 832 contigs and 3939 singletons, covering 21X genome equivalents. These contigs spanned a physical length of 689.8 Mb (~2.3X of the genome suggesting the complex heterozygosity of the genome. A set of 197 markers was developed from ESTs induced by drought-stress, or involved in anthocyanin biosynthesis or floral development, and was integrated into the physical map. Among these were 87 genetically mapped markers that anchored 54 contigs, spanning 76.4 Mb (25.5% across the genome. Analysis of a selection of 12,086 BAC end sequences (BESs from the minimal tiling path (MTP allowed a preview of the Aquilegia genome organization, including identification of transposable elements, simple sequence repeats and gene content. Common repetitive elements previously reported in both monocots and core eudicots were identified in Aquilegia suggesting the value of this genome in connecting the two major plant clades. Comparison with sequenced plant genomes

  14. Transforming Resource Sharing Services at an Australian Academic Library: The Case of the University of Wollongong

    Science.gov (United States)

    Daly, Rebecca; Baker, Liz; McIntosh, Lisa

    2014-01-01

    In 2011 the University of Wollongong Library undertook a significant review of its Resource Sharing services. This was prompted by constraints in the systems supporting this service, changes to the Library's key suppliers, Infotrieve Australia and the British Library Document Supply Service, and the need to deliver effective library services…

  15. Current status of "Net Resource subject guide" of Chinese academic libraries

    OpenAIRE

    Qiao, Huan

    2002-01-01

    Chinese Academic Library and Information System (CALIS), one of the most important public service system projects, was authorized and supported financially by the State Council of China. In order to integrate national investment, modern library service and advanced technology, abundant document resources of academic libraries and human resource, CALIS devotes to document guaranteed system construction, with focus on Chinese higher education digital library development. CALIS promotes informat...

  16. Utilization of Super BAC Pools and Fluidigm Access Array Platform for High-Throughput BAC Clone Identification: Proof of Concept

    Directory of Open Access Journals (Sweden)

    Peter J. Maughan

    2012-01-01

    Full Text Available Bacterial artificial chromosome (BAC libraries are critical for identifying full-length genomic sequences, correlating genetic and physical maps, and comparative genomics. Here we describe the utilization of the Fluidigm access array genotyping system in conjunction with KASPar genotyping technology to identify individual BAC clones corresponding to specific single-nucleotide polymorphisms (SNPs from an Amplicon Express seven-plate super pooled Amaranthus hypochondriacus BAC library. Ninety-six SNP loci, spanning the length of A. hypochondriacus linkage groups 1, 2, and 15, were simultaneously tested for clone identification from four BAC super pools, corresponding to 28 384-well plates, using a single Fluidigm integrated fluidic chip (IFC. Forty-six percent of the SNPs were associated with a single unambiguous identified BAC clone. PCR amplification and next-generation sequencing of individual BAC clones confirmed the IFC clone identification. Utilization of the Fluidigm Dynamic array platform allowed for the simultaneous PCR screening of 10,752 BAC pools for 96 SNP tag sites in less than three hours at a cost of ~$0.05 per reaction.

  17. Cost-benefit analysis of electronic information resources: an evaluation on Middle East Technical University Library.

    OpenAIRE

    BATI, Hacer

    2006-01-01

    In recent years there has been a rapid transition from print to electronic resources. Significant amount of library budgets are allocated for licensing electronic resources. Identifying and analyzing the benefits and costs of this new trend is therefore important for academic libraries. In this presentation experiences of METU (Middle East Technical University) Library on licensing and usage of electronic resources is evaluated by providing a cost-benefit analysis based on the cost and usage ...

  18. Rapid construction of a Bacterial Artificial Chromosomal (BAC) expression vector using designer DNA fragments.

    Science.gov (United States)

    Chen, Chao; Zhao, Xinqing; Jin, Yingyu; Zhao, Zongbao Kent; Suh, Joo-Won

    2014-11-01

    Bacterial artificial chromosomal (BAC) vectors are increasingly being used in cloning large DNA fragments containing complex biosynthetic pathways to facilitate heterologous production of microbial metabolites for drug development. To express inserted genes using Streptomyces species as the production hosts, an integration expression cassette is required to be inserted into the BAC vector, which includes genetic elements encoding a phage-specific attachment site, an integrase, an origin of transfer, a selection marker and a promoter. Due to the large sizes of DNA inserted into the BAC vectors, it is normally inefficient and time-consuming to assemble these fragments by routine PCR amplifications and restriction-ligations. Here we present a rapid method to insert fragments to construct BAC-based expression vectors. A DNA fragment of about 130 bp was designed, which contains upstream and downstream homologous sequences of both BAC vector and pIB139 plasmid carrying the whole integration expression cassette. In-Fusion cloning was performed using the designer DNA fragment to modify pIB139, followed by λ-RED-mediated recombination to obtain the BAC-based expression vector. We demonstrated the effectiveness of this method by rapid construction of a BAC-based expression vector with an insert of about 120 kb that contains the entire gene cluster for biosynthesis of immunosuppressant FK506. The empty BAC-based expression vector constructed in this study can be conveniently used for construction of BAC libraries using either microbial pure culture or environmental DNA, and the selected BAC clones can be directly used for heterologous expression. Alternatively, if a BAC library has already been constructed using a commercial BAC vector, the selected BAC vectors can be manipulated using the method described here to get the BAC-based expression vectors with desired gene clusters for heterologous expression. The rapid construction of a BAC-based expression vector facilitates

  19. BacMet

    DEFF Research Database (Denmark)

    Pal, Chandan; Bengtsson-Palme, Johan; Rensing, Christopher Günther T;

    2014-01-01

    -selection. Information on metal and biocide resistance genes, including their sequences and molecular functions, is, however, scattered. Here, we introduce BacMet (http://bacmet.biomedicine.gu.se) - a manually curated database of antibacterial biocide- and metal-resistance genes based on an in-depth review...

  20. SmartOrBAC

    Directory of Open Access Journals (Sweden)

    Imane BOUIJ-PASQUIER

    2015-11-01

    Full Text Available The emergence of the Internet of Things (IoT paradigm, provides a huge scope for more streamlined living through an increase of smart services but this coincides with an increase in security and privacy concerns, therefore access control has been an important factor in the development of IoT. This work proposes an authorization access model called SmartOrBAC built around a set of security and performance requirements. This model enhances the existing OrBAC (Organization-based Access Control model and adapts it to IoT environments. SmartOrBAC separates the problem into different functional layers and then distributes processing costs between constrained devices and less constrained ones and at the same time addresses the collaborative aspect with a specific solution. This paper also presents the application of SmartOrBAC on a real example of IoT and gives a complexity study demonstrating that even though this model is extensive, it does not add additional complexity regarding traditional access control models.

  1. BAC LIBRARY SCREENING OF PORCINE MILK PROTEIN GENES%利用细菌人工染色体文库(BAC)筛选猪乳蛋白基因

    Institute of Scientific and Technical Information of China (English)

    赵兴波; 铃木恒平; 李宁; 吴常信

    2001-01-01

    Gene clones of porcine Whey Acidic Protein(WAP),α s1-casein,α s2-casein,β-casein and κ-casein were selected and picked up using porcine bacteria artificial chromosome library (pBAC)and its two step-four dimensional screening system,and then discussed the one step-five dimensional screening system of pBAC library.%利用猪细菌人工染色体文库(porcine Bacteria Artificial Chromosome,pBAC)的两步-四维PCR(two step-4-D PCR)方法筛选猪乳清酸性蛋白(Whey Acidic Protein,WAP),αsl-酪蛋白,αs2-酪蛋白,β-酪蛋白,κ-酪蛋白的基因pBAC克隆,并提出了BAC基因文库一步-五维PCR(one step-5-D PCR)筛选基因的方法。

  2. Expanding Roles and Resources: Assessing the Collaboration between Florida Atlantic University Libraries and Taras Oceanographic Foundation

    Science.gov (United States)

    Arrieta, Diane; Brunnick, Barbara; Plocharczyk, Leah

    2015-01-01

    As academic libraries struggle to remain relevant when technological advancements and electronic resources threaten to make them obsolete, libraries are learning to re-invent themselves by molding and adapting staff skills to cultivate innovative outreach programs. The Science Outreach Committee of the John D. MacArthur Campus library at Florida…

  3. Using Pedestrian Choice Research to Facilitate Resource Engagement in a Midsized Academic Library

    Science.gov (United States)

    van Beynen, Kaya; Pettijohn, Patricia; Carrel, Marcy

    2010-01-01

    For a 1-year period, visitors to the Nelson Poynter Memorial Library, University of South Florida St. Petersburg were observed regarding how they negotiated through the first floor and interacted with the library resources and educational displays. Pedestrian choice research was applied to the library to better understand visitor movement and…

  4. Preservation and Conservation of Information Resources in the University of Zambia Library

    Science.gov (United States)

    Kanyengo, Christine Wamunyima

    2009-01-01

    Preservation and conservation of library materials is an important aspect of library and information management. Their importance and necessity are more paramount in countries where resources are limited and libraries need to balance them with the needs of an ever increasing number of students hoping to use them. This article reports on the…

  5. Hacking Blackboard: Customizing Access to Library Resources through the Blackboard Course Management System

    Science.gov (United States)

    Kellam, Lynda M.; Cox, Richard; Winkler, Hannah

    2009-01-01

    Academic libraries have long been trying to gain access to users through their favorite online spaces, such as social networking sites. In this article a project of the University of North Carolina at Greensboro's University Libraries, which integrated library resources with the campus course management system, Blackboard, is detailed. The main…

  6. Performance Evaluation of Karnataka State University Library Resources and Services:A Librarians Approach

    OpenAIRE

    Ramesha; B.D. Kumbar

    2004-01-01

    Library service is the supreme activity to attract the attention of the user community. It is more so in the case of a university library. Looking at the commitment of the university library to promote the cause of healthy higher education, it is essential on the part of the library professionals to evaluate and assesses the usability of the library resources in the light of the vast amount of investment made in acquiring the information resources and the cost of the manpower in managing the ...

  7. The Impact of Library Resources and Services on the Scholarly Activity of Medical Faculty and Residents.

    Science.gov (United States)

    Quesenberry, Alexandria C; Oelschlegel, Sandy; Earl, Martha; Leonard, Kelsey; Vaughn, Cynthia J

    2016-01-01

    Librarians at an academic medical center library gathered data to determine if library services and resources impacted scholarly activity. A survey was developed and sent out to faculty and residents asking how they used the library during scholarly activity. Sixty-five faculty members and residents responded to the survey. The majority of respondents involved with scholarly activity use the library's services and resources. PubMed is the most frequently used database. The positive results show the library impacts the scholarly activity of medical faculty and residents. PMID:27391176

  8. The Impact of Library Resources and Services on the Scholarly Activity of Medical Faculty and Residents.

    Science.gov (United States)

    Quesenberry, Alexandria C; Oelschlegel, Sandy; Earl, Martha; Leonard, Kelsey; Vaughn, Cynthia J

    2016-01-01

    Librarians at an academic medical center library gathered data to determine if library services and resources impacted scholarly activity. A survey was developed and sent out to faculty and residents asking how they used the library during scholarly activity. Sixty-five faculty members and residents responded to the survey. The majority of respondents involved with scholarly activity use the library's services and resources. PubMed is the most frequently used database. The positive results show the library impacts the scholarly activity of medical faculty and residents.

  9. Measuring the Value of Library Resources and Student Academic Performance through Relational Datasets

    Directory of Open Access Journals (Sweden)

    Margie Jantti

    2013-06-01

    Full Text Available Objective – This article describes a project undertaken by the University of Wollongong Library (UWL to identify whether a correlation exists between usage of library resources and academic performance.Methods – A multidimensional approach to systems design was implemented, requiring collaboration between among the library, university administration, Performance Indicator Project team (PIP, and information technology services. The project centers on the integration and interrogation of a series of discrete datasets containing student performance, attrition, demographic, borrowing, and electronic resources usage data. PIP built a cube for the library that links usage of library resources to student demographic data and academic performance (the “Library Cube”. Other cubes will be linked later.Results – While initial reports are rudimentary and do not yet incorporate data on e-resource usage, results are favourable in demonstrating the value of using the library information resources in coursework. Based on the data generated to date, students who borrow library resources do outperform students who do not. Early trend data shows up to a 12-point difference in grades.Conclusion – The Library Cube signals a new milestone in the UWL’s quality assessment journey. Well-established measures of effectiveness and efficiency will be further complemented by measures of impact and value, allowing the library to step even closer to the goal of having effective and valued partnerships with the university community to realize teaching, learning, research, and internalization goals.

  10. A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni

    Directory of Open Access Journals (Sweden)

    Coen M Adema

    2006-10-01

    Full Text Available To provide a novel resource for analysis of the genome of Biomphalaria glabrata, members of the international Biomphalaria glabrata Genome Initiative (biology.unm.edu/biomphalaria-genome.html, working with the Arizona Genomics Institute (AGI and supported by the National Human Genome Research Institute (NHGRI, produced a high quality bacterial artificial chromosome (BAC library. The BB02 strain B. glabrata, a field isolate (Belo Horizonte, Minas Gerais, Brasil that is susceptible to several strains of Schistosoma mansoni, was selfed for two generations to reduce haplotype diversity in the offspring. High molecular weight DNA was isolated from ovotestes of 40 snails, partially digested with HindIII, and ligated into pAGIBAC1 vector. The resulting B. glabrata BAC library (BG_BBa consists of 61824 clones (136.3 kb average insert size and provides 9.05 × coverage of the 931 Mb genome. Probing with single/low copy number genes from B. glabrata and fingerprinting of selected BAC clones indicated that the BAC library sufficiently represents the gene complement. BAC end sequence data (514 reads, 299860 nt indicated that the genome of B. glabrata contains ~ 63% AT, and disclosed several novel genes, transposable elements, and groups of high frequency sequence elements. This BG_BBa BAC library, available from AGI at cost to the research community, gains in relevance because BB02 strain B. glabrata is targeted whole genome sequencing by NHGRI.

  11. GenMapDB: a database of mapped human BAC clones

    OpenAIRE

    Morley, Michael; Arcaro, Melissa; Burdick, Joshua; Yonescu, Raluca; Reid, Thomas; Kirsch, Ilan R.; Cheung, Vivian G.

    2001-01-01

    GenMapDB (http://genomics.med.upenn.edu/genmapdb) is a repository of human bacterial artificial chromosome (BAC) clones mapped by our laboratory to sequence-tagged site markers. Currently, GenMapDB contains over 3000 mapped clones that span 19 chromosomes, chromosomes 2, 4, 5, 9–22, X and Y. This database provides positional information about human BAC clones from the RPCI-11 human male BAC library. It also contains restriction fragment analysis data and end sequen...

  12. Listening to Students: Customer Journey Mapping at Birmingham City University Library and Learning Resources

    Science.gov (United States)

    Andrews, Judith; Eade, Eleanor

    2013-01-01

    Birmingham City University's Library and Learning Resources' strategic aim is to improve student satisfaction. A key element is the achievement of the Customer Excellence Standard. An important component of the standard is the mapping of services to improve quality. Library and Learning Resources has developed a methodology to map these…

  13. End User Resource Valuation in Community College Libraries: A Q Methodology Study

    Science.gov (United States)

    Lucy, Theodore John

    2010-01-01

    The purpose of this study was to explore the valuation process of community college library end-users as they decide which information resources to use when conducting research. This study was designed as an exploratory study using Q methodology and focused on five specific information resources that community college library end-users routinely…

  14. Re-engineering the management of human resources in university libraries

    OpenAIRE

    Francis, A. T.; Humayoon Kabir, S.

    2008-01-01

    Information and Communication Technology application in university libraries poses several issues to be addressed, including those related to the management of human resources. As part of a study on the technology management of university libraries in Kerala, the aspect of human resources was also evaluated. A radical re-design in the prevailing system of management is advocated by the study. As a key component in the proper application of modern technologies in university libraries, human re...

  15. Resource Sharing in Research Libraries of Latin America

    OpenAIRE

    Lau, Jesús

    1995-01-01

    Research and academic libraries are the most developed information centers in Latin America. Their parent organizations are usually universities, government, and to a less extent private companies. Their library collections are therefore among the best. However, due to several factors, library collaboration and cooperation is limited, especially due to poor telecommunication links. Collections built by research and academic libraries evolve in isolation from one another, a factor that red...

  16. Annotation and BAC/PAC localization of nonredundant ESTs from drought-stressed seedlings of an indica rice

    Indian Academy of Sciences (India)

    P. Ravindra Babu; A. Chandra Sekhar; Nagabhushana Ithal; G. Markandeya; Arjula R. Reddy

    2002-04-01

    To decipher the genes associated with drought stress response and to identify novel genes in rice, we utilized 1540 high-quality expressed sequence tags (ESTs) for functional annotation and mapping to rice genomic sequences. These ESTs were generated earlier by 3′-end single-pass sequencing of 2000 cDNA clones from normalized cDNA libraries constructed from drought-stressed seedlings of an indica rice. A rice UniGene set of 1025 transcripts was constructed from this collection through the BLASTN algorithm. Putative functions of 559 nonredundant ESTs were identified by BLAST similarity search against public databases. Putative functions were assigned at a stringency E value of 10-6 in BLASTN and BLASTX algorithms. To understand the gene structure and function further, we have utilized the publicly available finished and unfinished rice BAC/PAC (BAC, bacterial artificial chromosome; PAC, P1 artificial chromosome) sequences for similarity search using the BLASTN algorithm. Further, 603 nonredundant ESTs have been mapped to BAC/PAC clones. BAC clones were assigned by a homology of above 95% identity along 90% of EST sequence length in the aligned region. In all, 700 ESTs showed rice EST hits in GenBank. Of the 325 novel ESTs, 128 were localized to BAC clones. In addition, 127 ESTs with identified putative functions but with no homology in IRGSP (International Rice Genome Sequencing Program) BAC/PAC sequences were mapped to the Chinese WGS (whole genome shotgun contigs) draft sequence of the rice genome. Functional annotation uncovered about a hundred candidate ESTs associated with abiotic stress in rice and Arabidopsis that were previously reported based on microarray analysis and other studies. This study is a major effort in identifying genes associated with drought stress response and will serve as a resource to rice geneticists and molecular biologists.

  17. Users' Satisfaction with Library Information Resources and Services: A Case Study College of Health Sciences Library Niger Delta University, Amassoma, Nigeria

    Science.gov (United States)

    Tiemo, Pereware Aghwotu; Ateboh, Benedict Alaowei

    2016-01-01

    This study investigated users' satisfaction with library information resources and services at the College of Health Sciences (CHS) library Niger Delta University, Nigeria. The objective was to determine the level of users satisfaction with library information resources and services. 2 (two) research questions were formulated to guide the study.…

  18. Performance Evaluation of Karnataka State University Library Resources and Services:A Librarians Approach

    Directory of Open Access Journals (Sweden)

    Ramesha

    2004-03-01

    Full Text Available Library service is the supreme activity to attract the attention of the user community. It is more so in the case of a university library. Looking at the commitment of the university library to promote the cause of healthy higher education, it is essential on the part of the library professionals to evaluate and assesses the usability of the library resources in the light of the vast amount of investment made in acquiring the information resources and the cost of the manpower in managing the library activity. This paper reveals one such attempt with reference to the university libraries in the state of Karnataka which is a part of Independent India.

  19. Identification of three wheat globulin genes by screening a Triticum aestivum BAC genomic library with cDNA from a diabetes-associated globulin

    Directory of Open Access Journals (Sweden)

    MacFarlane Amanda J

    2009-07-01

    Full Text Available Abstract Background Exposure to dietary wheat proteins in genetically susceptible individuals has been associated with increased risk for the development of Type 1 diabetes (T1D. Recently, a wheat protein encoded by cDNA WP5212 has been shown to be antigenic in mice, rats and humans with autoimmune T1D. To investigate the genomic origin of the identified wheat protein cDNA, a hexaploid wheat genomic library from Glenlea cultivar was screened. Results Three unique wheat globulin genes, Glo-3A, Glo3-B and Glo-3C, were identified. We describe the genomic structure of these genes and their expression pattern in wheat seeds. The Glo-3A gene shared 99% identity with the cDNA of WP5212 at the nucleotide and deduced amino acid level, indicating that we have identified the gene(s encoding wheat protein WP5212. Southern analysis revealed the presence of multiple copies of Glo-3-like sequences in all wheat samples, including hexaploid, tetraploid and diploid species wheat seed. Aleurone and embryo tissue specificity of WP5212 gene expression, suggested by promoter region analysis, which demonstrated an absence of endosperm specific cis elements, was confirmed by immunofluorescence microscopy using anti-WP5212 antibodies. Conclusion Taken together, the results indicate that a diverse group of globulins exists in wheat, some of which could be associated with the pathogenesis of T1D in some susceptible individuals. These data expand our knowledge of specific wheat globulins and will enable further elucidation of their role in wheat biology and human health.

  20. The Best Cataloger is a Frustrated Library User: Cataloging Failure and the Underutilization of Library Resources

    Directory of Open Access Journals (Sweden)

    John W. Thompson

    2015-08-01

    Full Text Available This essay points out that inconsistencies in the assignment of subject headings and call number can lead to failure to retrieve relevant materials from our libraries. Today it is frequently asserted that bibliographic records cataloged by the Library of Congress or other approved libraries will not require review or editing in our local libraries. This paper provides clear, but by no means unique examples of “cataloging failure” and explains the implications of a policy to add unedited bibliographic records (from vendors such as OCLC to our library catalogs. The result is the omission of otherwise relevant titles from fairly routine searches.

  1. Open Education Resources: The New Paradigm in Academic Libraries

    OpenAIRE

    Carmen Mitchell; Melanie Chu

    2014-01-01

    Academic library budgets are contracting while library usage is increasing. How can academic libraries best help campuses reduce costs and better serve their communities? One strategy is collecting campus-created content online and making it available through the campus Institutional Repository. All faculty, including tenure track and adjuncts, at California State University San Marcos were invited to participate in a brief Web-based survey with both quantitative and qualitative questions. Wi...

  2. Legal Resources Information System for Information Agencies of Specialized Libraries

    CERN Document Server

    Nguyen, Phuc V

    2011-01-01

    In recent years, the rapid development of information technology and communication has a strong impact to industry information - the library. The mission of the industry when in fact the great social place to see the library as knowledge management. Vietnam is in the process of building the rule of law socialist orientation and improves the legal system. So in the current development process, the law library plays an important role in the retention, dissemination and provision of legal information service of legislative, executive and judiciary, particularly especially research, teaching and learning of law school. But the response of the legal information library information agencies remains limited compared to the increasing demand of users.

  3. We're All in This Together: Library Faculty and Staff and Their Reporting of Electronic Resource Problems

    Science.gov (United States)

    Foster, Anita; Williams, Sarah C.

    2010-01-01

    Libraries continue to shift budgets toward obtaining more electronic resources. Electronic resources can develop problems at any time when a library offers access. Staff collaboration is vital in ensuring availability to those resources. Partnering with areas in the library that work most closely with patrons can help share the load of the…

  4. To get or not to get: the KAUST library e-resources acquisition experience

    KAUST Repository

    Ramli, Rindra M.

    2014-06-01

    In the challenging times of budget cuts and reviews, libraries are faced with issues, among others, such as justifying acquisition, negotiating deals and reviewing current subscriptions (pertaining to electronic resources). With the rapid increase in growth of electronic resources, libraries have to continuously assess their acquisition models and policies to constantly ensure that they are balancing their budget and users’ needs as well. This paper highlights the role played by Technical and IT department of King Abdullah University of Science and Technology (KAUST) library (Saudi Arabia) in acquiring the electronic resources (electronic books, electronic journals, databases, image and reference) for the community. It will describe the processes during the early days when KAUST library was inaugurated; how electronic resources were acquired and what went through during those days. The paper will elaborate further how the acquisition model has evolved and the various important roles played by the library staff in ensuring that acquisitions/subscriptions are justified, within the budget and provides ROI for the library. King Abdullah University is a graduate research university which opened in September 2009 with its first cohort of 800 graduate students (25% female) taught by 100 faculties. The focus of study and research in the university are: Mathematics and Computer Science, Physical Sciences and Life Sciences. The university library started with 10 staff. The library has a “state-of-the-art learning and information resource center supporting graduate education and advanced scientific research” (KAUST, 2010).

  5. Public Library Use of Free E-Resources

    Science.gov (United States)

    Hill, Heather; Bossaller, Jenny

    2013-01-01

    This article describes a multi-method research project examining the use of various freely available online collections and projects, such as Project Gutenberg, the Internet Archive, and Creative Commons-licensed ebooks, by public libraries. This research begins with the questions: what are libraries doing with freely available materials? Are…

  6. [Primary care resources available in digital libraries in Spanish Autonomous Regions].

    Science.gov (United States)

    Juan-Quilis, Verónica

    2013-03-01

    The Statement by the Spanish Society of Family and Community Medicine (SemFYC) on access to scientific information, highlights the need for providing digital libraries with certain resources in Autonomous Regions. The primary goal is to study the evidence-based medicine (EBM) coverage that SemFYC recommends regional virtual libraries. The regional health virtual libraries were identified and the access provided to health professionals, Internet presence, remote access and resources were studied. The results suggest there is ample coverage in 8 Autonomous Regions. At the top of the list was, Health Sciences Virtual Library of Navarre, the Balearic Islands Health Sciences Virtual Library, and Virtual Library of the Andalusian Public Health System. The present study needs to be extended to the other biomedical sciences, in order to obtain more accurate results.

  7. Sequencing of 6.7 Mb of the melon genome using a BAC pooling strategy

    Directory of Open Access Journals (Sweden)

    Garcia-Mas Jordi

    2010-11-01

    Full Text Available Abstract Background Cucumis melo (melon belongs to the Cucurbitaceae family, whose economic importance among horticulture crops is second only to Solanaceae. Melon has a high intra-specific genetic variation, morphologic diversity and a small genome size (454 Mb, which make it suitable for a great variety of molecular and genetic studies. A number of genetic and genomic resources have already been developed, such as several genetic maps, BAC genomic libraries, a BAC-based physical map and EST collections. Sequence information would be invaluable to complete the picture of the melon genomic landscape, furthering our understanding of this species' evolution from its relatives and providing an important genetic tool. However, to this day there is little sequence data available, only a few melon genes and genomic regions are deposited in public databases. The development of massively parallel sequencing methods allows envisaging new strategies to obtain long fragments of genomic sequence at higher speed and lower cost than previous Sanger-based methods. Results In order to gain insight into the structure of a significant portion of the melon genome we set out to perform massive sequencing of pools of BAC clones. For this, a set of 57 BAC clones from a double haploid line was sequenced in two pools with the 454 system using both shotgun and paired-end approaches. The final assembly consists of an estimated 95% of the actual size of the melon BAC clones, with most likely complete sequences for 50 of the BACs, and a total sequence coverage of 39x. The accuracy of the assembly was assessed by comparing the previously available Sanger sequence of one of the BACs against its 454 sequence, and the polymorphisms found involved only 1.7 differences every 10,000 bp that were localized in 15 homopolymeric regions and two dinucleotide tandem repeats. Overall, the study provides approximately 6.7 Mb or 1.5% of the melon genome. The analysis of this new data has

  8. Developing as new search engine and browser for libraries to search and organize the World Wide Web library resources

    OpenAIRE

    Sreenivasulu, V.

    2000-01-01

    Internet Granthalaya urges world wide advocates and targets at the task of creating a new search engine and dedicated browseer. Internet Granthalaya may be the ultimate search engine exclusively dedicated for every library use to search and organize the world wide web libary resources

  9. Screening and chromosome localization of two cotton BAC clones.

    Science.gov (United States)

    Cui, Xinglei; Liu, Fang; Liu, Yuling; Zhou, Zhongli; Wang, Chunying; Yanyan Zhao; Meng, Fei; Wang, Xingxing; Cai, Xiaoyan; Wang, Yuhong; Peng, Renhai; Wang, Kunbo

    2016-01-01

    Two bacterial artificial chromosome (BAC) clones (350B21 and 299N22) of Pima 90-53 cotton [Gossypium barbadense Linnaeus, 1753 (2n=4x=52)] were screened from a BAC library using SSR markers. Strong hybridization signals were detected at terminal regions of all A genome (sub-genome) chromosomes, but were almost absent in D genome (sub-genome) chromosomes with BAC clone 350B21 as the probe. The results indicate that specific sequences, which only exist at the terminal parts of A genome (sub-genome) chromosomes with a huge repeat number, may be contained in BAC clone 350B21. When utilizing FISH with the BAC clone 299N22 as probe, a pair of obvious signals was detected on chromosome 13 of D genome (sub-genome), while strong dispersed signals were detected on all A genome (sub-genome) chromosomes. The results showed that peculiar repetitive sequence, which was distributed throughout all A genome (sub-genome) chromosomes, may exist in BAC clone 299N22. The absence of the repetitive sequences, which exist in the two BAC clones, in D genome may account for the genome-size variation between A and D genomes. In addition, the microcolinearity analysis of the clone 299N22 and its homologous region on Gossypium raimondii Ulbrich, 1932 chromosome 13 (D513) indicated that the clone 299N22 might come from A sub-genome of sea island cotton (Gossypium barbadense), and a huge number of small deletions, illegitimate recombination, translocation and rearrangements may have occurred during the genus evolution. The two BAC clones studied here can be used as cytological markers but will be also be helpful to research in cotton genome evolution and comparative genomics. PMID:27186333

  10. Enhancing Teaching and Learning: Libraries and Open Educational Resources in the Classroom

    Science.gov (United States)

    Davis, Erin; Cochran, Dory; Fagerheim, Britt; Thoms, Becky

    2016-01-01

    Academic libraries continually adjust services to adapt to the ever-changing landscape in higher education. In response to the broken textbook market, libraries are becoming actively involved in the open educational resources (OER) movement. Although there is not a formal program in place, librarians at Utah State University explored a…

  11. Assessment of Data Management Services at New England Region Resource Libraries

    Directory of Open Access Journals (Sweden)

    Julie Goldman

    2015-07-01

    Full Text Available Objective: To understand how New England medical libraries are addressing scientific research data management and providing services to their communities. Setting: The National Network of Libraries of Medicine, New England Region (NN/LM NER contains 17 Resource Libraries. The University of Massachusetts Medical School serves as the New England Regional Medical Library (RML. Sixteen of the NER Resource Libraries completed this survey. Methods: A 40-question online survey assessed libraries’ services and programs for providing research data management education and support. Libraries shared their current plans and institutional challenges associated with developing data services. Results: This study shows few NER Resource Libraries currently integrate scientific research data management into their services and programs, and highlights the region’s use of resources provided by the NN/LM NER RML at the University of Massachusetts Medical School. Conclusions: Understanding the types of data services being delivered at NER libraries helps to inform the NN/LM NER about the eScience learning needs of New England medical librarians and helps in the planning of professional development programs that foster effective biomedical research data services.

  12. Cost of Electronic Reference Resources and LCM: The Library Costing Model.

    Science.gov (United States)

    Hayes, Robert M.

    1996-01-01

    Views assessment of "Costs of Electronic Reference Resources" both in a general descriptive framework and within the context of a specific model for costing of library operations and services called LCM, the Library Costing Model. Examples of costing data uses are provided, and categories of costs are related to types of electronic reference…

  13. The small and rural academic library leveraging resources and overcoming limitations

    CERN Document Server

    Davis Kendrick, Kaetrena

    2016-01-01

    Through the use of case studies, research, and practical interviews, The Small or Rural Academic Library: Leveraging Resources and Overcoming Limitations explores how academic librarians in such environments can keep pace with, create, and improve modern library practices and services, network with colleagues, and access continuing education and professional development opportunities.

  14. A New Model and Its Implementation of Academic Resources Services for Academic Libraries

    Directory of Open Access Journals (Sweden)

    Yu-Ting Chiang

    2006-12-01

    Full Text Available Internet has become one of the important channels for retrieval of academic resources in the recent years. The roles and service models of academic libraries has been changed accordingly. Most academic libraries have been providing subject directory (subject gateway for users to browse the highly selected academic resources. On the contrary, few academic libraries managed to provide academic resources for user to retrieve. This article proposes a new model which the libraries will cooperate with search engine providers to support the full-text retrieval for academic resources in a low cost. We discuss the design and implementation of “NTU Scholars Gateway” based on the new model. In addition, the functionalities and future development of NTU Scholars Gateway are discussedas well. [Article content in Chinese

  15. To Get or Not to Get the Kaust Library E-Resources Acquisition Experience

    OpenAIRE

    Ramli, Rindra M.; Kabli, Ola A.

    2014-01-01

    In the challenging times of budget cuts and reviews, libraries are faced with issues such as justifying acquisition, negotiating deals and reviewing current subscriptions (pertaining to electronic resources) among others. With the rapid growth of electronic resources, libraries have to continuously assess their acquisition models and policies to constantly ensure that they are balancing their budget and users’ needs as well. This paper highlights the role played by Technical and Digital Servi...

  16. Library Electronic Resource Sharing Among Liberal Arts Colleges: ACS Palladian Alliance Project

    Directory of Open Access Journals (Sweden)

    Wenxian Zhang

    1997-03-01

    Full Text Available 無Effective electronic resource sharing is critical to library information services of the 1990s. Explosion of data and increased cost of information force libraries to work together, and technological advancements present the library service profession a platform for resource sharing. The Palladian Alliance Project of the Associated Colleges of the South is designed to provides ACS member institutions an effective means to enhance information access for their faculty and students, and achieve significant cost containment in the years to come.

  17. Caught in the Web: The Impact of Library Instruction on Business Students' Perceptions and Use of Print and Online Resources.

    Science.gov (United States)

    Lombardo, Shawn V.; Miree, Cynthia E.

    2003-01-01

    Describes a study that investigated whether library instruction could impact undergraduate business students' attitudes and uses of three information formats: print materials, library databases, and Web resources. Results indicate that after library instruction, student held more favorable attitudes toward print resources and used them more than…

  18. A comparative study on users’ satisfaction of library resource sharing under the circumstance of network in Nanjing

    Institute of Scientific and Technical Information of China (English)

    ZHU; Suoling; LIU; Lei

    2009-01-01

    From the perspective of information resource sharing,the authors investigated the ratio and degree of users’satisfaction with resource sharing among college libraries,public libraries and research libraries in Nanjing city.Based on the analysis and discussion about the findings,the authors summarized the library resource sharing system in satisfying users’information requirements,including its pros and cons.At last,further considerations have been provided on how to improve library resource sharing system in the region.

  19. Leveraging Standards To Enhance the Library's Goals and Resources.

    Science.gov (United States)

    Marriott, Catherine

    2002-01-01

    Discussion of the use of technology in primary grades focuses on a pilot project in the Orchard Park (New York) elementary schools that used computers to teach a nonfiction unit for English language arts, focusing on the New York State learning standards. Describes the role of school library media specialists and benefits to students. (LRW)

  20. Factors Influencing Virtual Patron satisfaction with Online Library Resources and Services

    Directory of Open Access Journals (Sweden)

    Katherine Tyler

    2011-07-01

    Full Text Available College students are accessing virtual libraries whether they are on campus or learning from a distance. Academic institutions serving virtual patrons must remain focused on meeting the needs of those library users by continually examining their preferences, their searching behavior, and the information they seek. The purpose of this research was to determine if virtual patrons are satisfied with the resources and services being provided by a university’s online library. Following a web-based survey, demographic characteristics of students were analyzed to determine if any influenced students’ satisfaction. Using analysis of variance, correlation, and descriptive statistics, several demographic factors were found to influence student satisfaction with the library’s online resources: age, gender, achieved educational level, student status, and computer experience. One factor, computer experience, was found to influence student satisfaction with the library’s online services.Overall, students reported satisfaction with the university’s online library resources and services. Comments submitted to open-ended questions regarding areas for improvement to the online library provide library administrators with avenues for development to increase awareness of library services, focus improvement in navigation, and enhance student satisfaction.

  1. Managing information resources in libraries collection management in theory and practice

    CERN Document Server

    Clayton, Peter

    2006-01-01

    The management of information resources in libraries is of greater importance in the digital world. This book encompasses different areas of collection management and cover topics, such as: collection management in the organizational context; collection development policies; selection principles and resources; budget management; and more.

  2. HIV/AIDS Information Resources from the National Library of Medicine-STOP

    Energy Technology Data Exchange (ETDEWEB)

    Templin-Branner, W. and N. Dancy

    2010-06-15

    The HIV/AIDS Information Resources from the National Library of Medicine training is designed specifically for the UNCFSP HBCU Screening, Testing, Outreach, and Prevention (STOP) HIV/AIDS Program project members to provide valuable health information resources from the National Library of Medicine and other reliable sources to increase awareness of the wealth of treatment information and educational materials that are available on the Internet and to improve prevention and treatment education for their clients. These resources will also meet the needs of community-based organizations

  3. The Study of Analytical Model of Library Electronic Resources Usage-A Case of Medical Electronic Resources

    Directory of Open Access Journals (Sweden)

    Chung-Yen Yu

    2014-10-01

    Full Text Available With the advents of internet, the importance of electronic resources is growing. Due to the increasing expensiveness of electronic resources, university libraries normally received budgets from parent institutions annually. They necessarily applied effective and systematic methods for decision making in electronic resources purchase or re-subscription. However, there are some difficulties in practices: First of all, libraries are unable to receive user records; second, the COUNTER statistics does not include details about users and their affiliation. As a result, one cannot conduct advanced user analysis based on the usage of users, institutions, and departments. To overcome the difficulties, this study presents a feasible model to analyze electronic resource usage effectively and flexibly. We set up a proxy server to collect actual usage raw data. By analyzing items in internet browsing records, associated with original library automatic system, this study aims at exploring how to use effective ways to analyze big data of website log data. We also propose the process of how original data to be transformed, cleared, integrated, and demonstrated. This study adopted a medical university library and its subscription of medical electronic resources as a case. Our data analysis includes (1 year of subscription,(2 title of journal, (3 affiliation, (4 subjects, and (5 specific journal requirements, etc. The findings of the study are contributed to obtain further understanding in policy making and user behavior analysis. The integrated data provides multiple applications in informatics research, information behavior, bibliomining, presenting diverse views and extended issues for further discussion.

  4. International Students' Perception of Library Services and Information Resources in Chinese Academic Libraries

    Science.gov (United States)

    Nzivo, Charles N.; Chuanfu, Chen

    2013-01-01

    With the growth of international student enrollment in Chinese universities, user perception has become an area of developing research in academic libraries. China's policy of opening up has led to the education and economic reforms allowing major universities to enroll international students. These changes have created an increasing need for…

  5. The Open Library at AU (Athabasca University: Supporting Open Access and Open Educational Resources

    Directory of Open Access Journals (Sweden)

    Colin Elliott

    2015-04-01

    Full Text Available To address challenges that learners, course creators, librarians and academics involved with OER and MOOCs are facing when looking for scholarly materials, Athabasca University Library has initiated the development of the Open Library at AU. This open library is a full library website that provides easy access to open and free resources. Tools and information literacy tutorials are also included to enable learners, researchers, and others to find, evaluate, and use the information they need for their open learning course or research. Many of the challenges that those involved in open learning face are addressed by the open library and the potential impact it can have on open learning and knowledge sharing is tremendous. http://dx.doi.org/10.5944/openpraxis.7.2.196

  6. Publishing, Books and Library Resources: Brazil and Argentina.

    Science.gov (United States)

    National Book Committee, Inc., New York, NY.

    This survey synthesizes and analyzes the book resources and book requirements of Brazil, with particular reference to the educational scene, in terms of local production and distribution resources and capabilities. Information on the geography, political establishment, economy, and educational system of Brazil is presented in the introductory…

  7. Use of Library Resources and Services by UG Students -A Study of NIT, Kurukshetra

    OpenAIRE

    Sushila Kumari; Prem Kumar; Anil Kumar

    2013-01-01

    The present study tries to know the use of library resources and services by UG Students of NIT,Kurukshetra. On the basis of the streams, 141 students were selected with the help of Stratified Sampling. The studyhighlights that the students visit the Library mainly for preparation of their course work and assignments (83.68%),examinations notes and tests (66.66%), preparation for workshops (39.71%) and tutorial presentations (16.31%).Maximum students considered that library collections (86.5%...

  8. Cost-Benefit Analysis of Electronic Information Resources: An Evaluation of the Middle East Technical University Library

    OpenAIRE

    Batı, Hacer

    2006-01-01

    In recent years there has been a rapid transition from print to electronic resources. Significant amount of library budgets are allocated for licensing electronic resources. Identifying and analyzing the benefits and costs of this new trend is therefore important for academic libraries. In this presentation experiences of METU (Middle East Technical University) Library on licensing and usage of electronic resources is evaluated by providing a cost-benefit analysis based on the cost and ...

  9. Human resource management and career planning in a larger library

    OpenAIRE

    Jelka Gazvoda

    1997-01-01

    Human resource management is presented as a managerial function which is used to develop potential abilities of the employees to achieve organizational goals.Different perception of the employees is essential - people working in the organization are treated as capital and not as an expenditure. In human resource management the most important view of the employees is their potential growth and professional development, training for acquiring new responsibilities and encouragement for innovatio...

  10. Linking Formal and Informal Science Education: A Successful Model using Libraries, Volunteers and NASA Resources

    Science.gov (United States)

    Race, M. S.; Lafayette Library; Learning Center Foundation (Lllcf)

    2011-12-01

    In these times of budget cuts, tight school schedules, and limited opportunities for student field trips and teacher professional development, it is especially difficult to expose elementary and middle school students to the latest STEM information-particularly in the space sciences. Using our library as a facilitator and catalyst, we built a volunteer-based, multi-faceted, curriculum-linked program for students and teachers in local middle schools (Grade 8) and showcased new astronomical and planetary science information using mainly NASA resources and volunteer effort. The project began with the idea of bringing free NASA photo exhibits (FETTU) to the Lafayette and Antioch Libraries for public display. Subsequently, the effort expanded by adding layers of activities that brought space and science information to teachers, students and the pubic at 5 libraries and schools in the 2 cities, one of which serves a diverse, underserved community. Overall, the effort (supported by a pilot grant from the Bechtel Foundation) included school and library based teacher workshops with resource materials; travelling space museum visits with hands-on activities (Chabot-to-Go); separate powerpoint presentations for students and adults at the library; and concurrent ancillary space-related themes for young children's programs at the library. This pilot project, based largely on the use of free government resources and online materials, demonstrated that volunteer-based, standards-linked STEM efforts can enhance curriculum at the middle school, with libraries serving a special role. Using this model, we subsequently also obtained a small NASA-Space Grant award to bring star parties and hand-on science activities to three libraries this Fall, linking with numerous Grade 5 teachers and students in two additional underserved areas of our county. It's not necessary to reinvent the wheel, you just collect the pieces and build on what you already have.

  11. The Physic Source, a ComPADRE digital library, focusing on resources for teaching introductory physics

    Science.gov (United States)

    Mzoughi, Taha

    2012-03-01

    The Physics Source (http://www.physicssource.org/), a component of ComPADRE, was designed to help faculty teaching introductory physics courses identify and use high quality relevant curriculum and pedagogical resources in their teaching. Faculty can use to Physics Source to find, share, and collaborate on building high-quality, effective teaching and learning materials. Users can also organize the content they like, and submit their own resources for inclusion in the library. Among the content included in the library are simulations, lab activities, classroom demonstrations, videos, information on some teaching techniques, and physics education research articles.

  12. Extending the scope of Trove: addition of e-resources subscribed to by Australian Libraries.

    OpenAIRE

    Holley, Rose

    2011-01-01

    Trove is the national discovery service for Australia managed by the National Library of Australia. It was released in December 2009. It contains metadata for millions of freely accessible items, from more than 1,000 contributing institutions. The focus is on Australia and Australians. In 2011 it was developed further to include selected sets of e-resources subscribed to by Australian libraries. Trove v4.0 was released in May 2011 after 120 million subscription e-resources were successfully i...

  13. Electronic Engineering Library In Sweden: A Quality Assessed Internet Resource from the Swedish Technological University Libraries

    OpenAIRE

    Lager, Gunnar; Nordlander, Marianne

    2012-01-01

    A status report of the plans and issues under discussion in creating this cooperative electronic national resource to support engineering education in Sweden. An opportunity to preview what is currently under construction for the EELS.

  14. Effective Techniques for the Promotion of Library Services and Resources

    Science.gov (United States)

    Yi, Zhixian

    2016-01-01

    Introduction: This study examines how Australian academic librarians perceive techniques for promoting services and resources, and the factors affecting the perceptions regarding effectiveness of techniques used. Method: Data were collected from an online survey that was sent to 400 academic librarians in thirty-seven Australian universities. The…

  15. Integration of multimedia content and e-learning resources in a digital library

    Science.gov (United States)

    Pascual, Mireia; Ferran, Núria; Minguillón, Julià

    2006-01-01

    In this paper we describe a proposal for multimedia and e-learning content description based on standards interoperability within a digital library environment integrated in a virtual campus. In any virtual e-learning environment, a complex scenario which usually includes a digital library or, at least, a repository of learning resources, different levels of description are needed for all the elements: learning resources, multimedia content, activities, roles, etc. These elements can be described using library, e-learning and multimedia standards, depending on the specific needs of each particular scenario of use, but this might lead to an undesirable duplication of metadata, and to inefficient content queries and maintenance. Furthermore, there is a lack of semantic descriptions which makes all these contents merely become digital objects in the digital library, without exploiting all the possibilities in a e-learning virtual environment. Due to its flexibility and completeness, we propose to use the MPEG-7 standard for describing all the learning resources in the digital library, combined with the use of an ontology for a formal description of the learning process. The equivalences of Dublin Core, LOM and MPEG-7 standards are outlined, and the requirements of a proposal for a MPEG-7 based representation for all the contents in the digital library and the virtual classroom are described. The intellectual property policies for content sharing both within and among organizations are also addressed. With such proposal, it would be possible to build complex multimedia courses from a repository of learning objects using the digital library as the core repository.

  16. QR Codes as Finding Aides: Linking Electronic and Print Library Resources

    Science.gov (United States)

    Kane, Danielle; Schneidewind, Jeff

    2011-01-01

    As part of a focused, methodical, and evaluative approach to emerging technologies, QR codes are one of many new technologies being used by the UC Irvine Libraries. QR codes provide simple connections between print and virtual resources. In summer 2010, a small task force began to investigate how QR codes could be used to provide information and…

  17. Implementation of an Electronic Resource Assessment System in an Academic Library

    Science.gov (United States)

    Xu, Fei

    2010-01-01

    Purpose: The purpose of this paper is to provide a library with useful information about selection criteria for an electronic resource assessment system and practical assistance on how to implement efficiently such a system. Design/methodology/approach: The paper is based on literature review, desk research, and implementation experience.…

  18. Remote Electronic Resources and the OPAC: Illustrated by the Unisa Library Experience.

    Science.gov (United States)

    Van der Merwe, Ina; Van Eeden, Welna; Hartzer, Sandra

    This paper describes the Unisa (University of South Africa) Library's experience with cataloging remote electronic resources, including electronic journals, electronic text files, online databases, digital images, Unisa campus Web sites, and mailing list discussions. The first section discusses the decision to add bibliographic references for…

  19. The Impact of Library Resource Utilization on Undergraduate Students' Academic Performance: A Propensity Score Matching Design

    Science.gov (United States)

    Kot, Felly Chiteng; Jones, Jennifer L.

    2015-01-01

    This study uses three cohorts of first-time, full-time undergraduate students (N = 8,652) at a large, metropolitan, public research university to examine the impact of student use of three library resources (workstations, study rooms, and research clinics) on academic performance. To deal with self-selection bias and estimate this impact more…

  20. Council on Library and Information Resources: Annual Report, 2007-2008

    Science.gov (United States)

    Council on Library and Information Resources, 2008

    2008-01-01

    This annual report of the Council on Library and Information Resources (CLIR) contains an overview of activities of the Council between July 1, 2007 and June 30, 2008. These include programs, advisory groups, grants and contracts, and financial statements. It also includes a list of staff, a letter from the chairperson Paula Kaufman, and a message…

  1. A Comparative BAC Map for the Gilthead Sea Bream (Sparus aurata L.

    Directory of Open Access Journals (Sweden)

    Heiner Kuhl

    2011-01-01

    Full Text Available This study presents the first comparative BAC map of the gilthead sea bream (Sparus aurata, a highly valuated marine aquaculture fish species in the Mediterranean. High-throughput end sequencing of a BAC library yielded 92,468 reads (60.6 Mbp. Comparative mapping was achieved by anchoring BAC end sequences to the three-spined stickleback (Gasterosteus aculeatus genome. BACs that were consistently ordered along the stickleback chromosomes accounted for 14,265 clones. A fraction of 5,249 BACs constituted a minimal tiling path that covers 73.5% of the stickleback chromosomes and 70.2% of the genes that have been annotated. The N50 size of 1,485 “BACtigs” consisting of redundant BACs is 337,253 bp. The largest BACtig covers 2.15 Mbp in the stickleback genome. According to the insert size distribution of mapped BACs the sea bream genome is 1.71-fold larger than the stickleback genome. These results represent a valuable tool to researchers in the field and may support future projects to elucidate the whole sea bream genome.

  2. Caribbean Equal Access Program: HIV/AIDS Information Resources from the National Library of Medicine

    Energy Technology Data Exchange (ETDEWEB)

    Nancy Dancy, NLM, and Wilma Templin-Branner, ORISE

    2009-01-01

    As the treatment and management of HIV/AIDS continues to evolve with new scientific breakthroughs, treatment discoveries, and management challenges, it is difficult for people living with HIV/AIDS and those who care for them to keep up with the latest information on HIV/AIDS prevention, treatment, and research. The National Library of Medicine, of the National Institutes of Health, has a wealth of health information resources freely available on the Internet to address these needs.

  3. Library and Information Resources and Users of Digital Resources in the Humanities

    Science.gov (United States)

    Warwick, Claire; Terras, Melissa; Galina, Isabel; Huntington, Paul; Pappa, Nikoleta

    2008-01-01

    Purpose: The purpose of this article is to discuss the results of the Log Analysis of Internet Resources in the Arts and Humanities (LAIRAH) study. It aims to concentrate upon the use and importance of information resources, physical research centres and digital finding aids in scholarly research. Design/methodology/approach: Results are presented…

  4. Building a Digital Library from the Ground Up: an Examination of Emergent Information Resources in the Machine Learning Community

    Science.gov (United States)

    Cunningham, Sally Jo

    The current crop of digital libraries for the computing community are strongly grounded in the conventional library paradigm: they provide indexes to support searching of collections of research papers. As such, these digital libraries are relatively impoverished; the present computing digital libraries omit many of the documents and resources that are currently available to computing researchers, and offer few browsing structures. These computing digital libraries were built 'top down': the resources and collection contents are forced to fit an existing digital library architecture. A 'bottom up' approach to digital library development would begin with an investigation of a community's information needs and available documents, and then design a library to organize those documents in such a way as to fulfill the community's needs. The 'home grown', informal information resources developed by and for the machine learning community are examined as a case study, to determine the types of information and document organizations 'native' to this group of researchers. The insights gained in this type of case study can be used to inform construction of a digital library tailored to this community.

  5. Out on the Web: The Relationship between Campus Climate and GLBT-Related Web-based Resources in Academic Libraries

    Science.gov (United States)

    Ciszek, Matthew P.

    2011-01-01

    This article explores the relationship between the perceived campus environment for gay, lesbian, bisexual, and transgender (GLBT) students at colleges and universities and how academic libraries have deployed GLBT-related resources on the Web. Recommendations are made for increasing GLBT-related materials and information in academic libraries.…

  6. 棉花细菌人工染色体文库构建方法探讨%Studies on Construction Method of Cotton Bacterial Artificial Chromosome Library

    Institute of Scientific and Technical Information of China (English)

    高海燕; 王省芬; 刘方; 彭仁海; 张艳; 马峙英; 王坤波

    2013-01-01

    细菌人工染色体(Bacterial artificial chromosome,BAC)文库是开展基因组测序、基因图位克隆、分子标记、物理作图等研究的重要基因组资源.本文在构建了二倍体野生棉阿非利加棉(Gossypium herbaceum var.africanum)BAC文库的基础上,就棉花细菌人工染色体基因组文库构建过程中高分子量基因组DNA的提取、部分酶切片段选择、DNA的回收、连接转化以及BAC文库的保存等过程中一些细节和注意事项进行了比较详细的分析比较,希望能为棉花BAC文库的构建提供一些可供借鉴的经验.%Bacterial artificial chromosome (BAC) library is an important genome resources to such research as genome sequencing, map-based cloning, molecular markers, and physical mapping. On the base of the construction of BAC library for Gossypi-um herbaceum var. africanum, this paper presents an exhaustive analysis on details and notices of the BAC library construction process. It includes extraction of high molecular weight (HMW) nuclear DNA, determination of the optimized enzyme for partial digestion of HMW DNA, two rounds of size fractionation, recovery of large fragments DNA, ligation and transformation of large fragments of DNA and storage of BAC library. Thus being able to supply an experience for constructing high efficiency cotton BAC library.

  7. A Study of Assessment of Library E-resource Metasearch System

    Directory of Open Access Journals (Sweden)

    Fan-Chuan Tseng

    2008-12-01

    Full Text Available As the emergent development of computer and network technology, digital library and information service has become an important approach for users to increase their academic as well as job performance due to the authorized, accurate, and reliable information. In the realm of information management, the exploration of factors influencing information system utilization and the evaluation of benefits are two critical issues. However, related studies focus more on the system functions and usually ignore the effects of individual cognition and social influence. In this study, we extend the IS success model with two additional factors-self-efficacy and subjective norm, trying to explore the potential influences on digital library usage, user satisfaction and performance.This research takes the Chang Jung Christian University library E-resources Metasearch system as the case study. A questionnaire was developed and finally got 160 useful student samples to test and verify the hypotheses. The research results confirmed that self-efficacy and subjective norm can significantly affect the usage of E-resource Metasearch System. Subsequently, the users feel satisfied with the system, and increase their academic performance. Finally, we provide discussion, implications and suggestions in two aspects – cooperating more with educational programs to increase users’ information literacy, and improving the customer-oriented system interface, functions, and services to enhance its quality. [Article content in Chinese

  8. The eTOX Library of Public Resources for in Silico Toxicity Prediction.

    Science.gov (United States)

    Cases, M; Pastor, M; Sanz, F

    2013-01-01

    (1000-1500 characters) In spite of the increasing amount of public access resources that offer original data related to drug toxicology, the successful exploitation of such data for the development of in silico predictive models is still limited by the quality of the data available, its integrability and its coverage for each toxicity endpoint. This work describes the strategy developed by the IMI eTOX consortium for identifying and compiling data and other related resources from the biomedical literature and a wide spectrum of public on-line sources. The main result of this effort is a large web-based structured library containing links to articles of toxicological relevance (data that can be used for modeling purposes, computational models, and toxicity mechanisms), public databases, standardized vocabularies and modeling tools. All this material has been manually reviewed, systematically evaluated and grouped into different categories. The library has been made public at the eTOX website (http://www.etoxproject.eu/), where it is updated on a monthly basis, constituting a useful resource for affording the in silico toxicity prediction of novel drug candidates.

  9. 高校图书资源共享探讨%A Discussion on Sharing of University Library Resources

    Institute of Scientific and Technical Information of China (English)

    张宏才

    2012-01-01

    On the base of the current library management and service system, the article analyses and discuses defects on university library resources management, and suggests that college and university should share all university library resources, and put forward realizing ways of sharing library resources.%高校图书馆在高校教学与科研工作中发挥着重要作用,而目前高校图书馆与各系图书室之间资源共享是一大难题。从高校目前图书资源管理的现状出发,探讨其存在的缺陷,从而实现高校内部图书资源共享管理,并采用必要的共享管理的新方式很有必要。

  10. BAC-HAPPY mapping (BAP mapping): a new and efficient protocol for physical mapping.

    Science.gov (United States)

    Vu, Giang T H; Dear, Paul H; Caligari, Peter D S; Wilkinson, Mike J

    2010-02-08

    Physical and linkage mapping underpin efforts to sequence and characterize the genomes of eukaryotic organisms by providing a skeleton framework for whole genome assembly. Hitherto, linkage and physical "contig" maps were generated independently prior to merging. Here, we develop a new and easy method, BAC HAPPY MAPPING (BAP mapping), that utilizes BAC library pools as a HAPPY mapping panel together with an Mbp-sized DNA panel to integrate the linkage and physical mapping efforts into one pipeline. Using Arabidopsis thaliana as an exemplar, a set of 40 Sequence Tagged Site (STS) markers spanning approximately 10% of chromosome 4 were simultaneously assembled onto a BAP map compiled using both a series of BAC pools each comprising 0.7x genome coverage and dilute (0.7x genome) samples of sheared genomic DNA. The resultant BAP map overcomes the need for polymorphic loci to separate genetic loci by recombination and allows physical mapping in segments of suppressed recombination that are difficult to analyze using traditional mapping techniques. Even virtual "BAC-HAPPY-mapping" to convert BAC landing data into BAC linkage contigs is possible.

  11. Toward a Molecular Cytogenetic Map for Cultivated Sunflower (Helianthus annuus L.) by Landed BAC/BIBAC Clones

    OpenAIRE

    Feng, Jiuhuan; Liu, Zhao; Cai, Xiwen; Jan, Chao-Chien

    2013-01-01

    Conventional karyotypes and various genetic linkage maps have been established in sunflower (Helianthus annuus L., 2n = 34). However, the relationship between linkage groups and individual chromosomes of sunflower remains unknown and has considerable relevance for the sunflower research community. Recently, a set of linkage group-specific bacterial /binary bacterial artificial chromosome (BAC/BIBAC) clones was identified from two complementary BAC and BIBAC libraries constructed for cultivate...

  12. Library

    OpenAIRE

    Dulaney, Ronald E. Jr.

    1997-01-01

    This study began with the desire to design a public town library of the future and became a search for an inkling of what is essential to Architecture. It is murky and full of contradictions. It asks more than it proposes, and the traces of its windings are better ordered through collage than logical synthesis. This study is neither a thesis nor a synthesis. When drawing out the measure of this study it may be beneficial to state what it attempts to place at the ...

  13. Herpesvirus BACs: Past, Present, and Future

    Directory of Open Access Journals (Sweden)

    Charles Warden

    2011-01-01

    Full Text Available The herpesviridae are a large family of DNA viruses with large and complicated genomes. Genetic manipulation and the generation of recombinant viruses have been extremely difficult. However, herpesvirus bacterial artificial chromosomes (BACs that were developed approximately 10 years ago have become useful and powerful genetic tools for generating recombinant viruses to study the biology and pathogenesis of herpesviruses. For example, BAC-directed deletion mutants are commonly used to determine the function and essentiality of viral genes. In this paper, we discuss the creation of herpesvirus BACs, functional analyses of herpesvirus mutants, and future applications for studies of herpesviruses. We describe commonly used methods to create and mutate herpesvirus BACs (such as site-directed mutagenesis and transposon mutagenesis. We also evaluate the potential future uses of viral BACs, including vaccine development and gene therapy.

  14. Assessing the Value of E-Resources to York University Faculty Using the MINES for Libraries Protocol: An Evolving Landscape

    Science.gov (United States)

    Lupton, Aaron; Davidson, Catherine

    2013-01-01

    This article describes how York University analyzed the results from its 2010-11 implementation of the Measuring the Impact of Networked Electronic Services for Libraries survey to measure value of investments in e-resources. The results were assessed through two variables: e-resources used by faculty when carrying out funded and non-funded…

  15. Providing Curriculum Support in the School Library Media Center: Resource Alignment, or How To Eat an Elephant.

    Science.gov (United States)

    Lowe, Karen

    2003-01-01

    Discusses the process of weeding, updating, and building a school library media collection that supports the state curriculum. Explains resource alignment, a process for using the shelf list as a tool to analyze and align media center resources to state curricula, and describes a five-year plan and its usefulness for additional funding. (LRW)

  16. Comparative BAC-based mapping in the white-throated sparrow, a novel behavioral genomics model, using interspecies overgo hybridization

    Directory of Open Access Journals (Sweden)

    Gonser Rusty A

    2011-06-01

    Full Text Available Abstract Background The genomics era has produced an arsenal of resources from sequenced organisms allowing researchers to target species that do not have comparable mapping and sequence information. These new "non-model" organisms offer unique opportunities to examine environmental effects on genomic patterns and processes. Here we use comparative mapping as a first step in characterizing the genome organization of a novel animal model, the white-throated sparrow (Zonotrichia albicollis, which occurs as white or tan morphs that exhibit alternative behaviors and physiology. Morph is determined by the presence or absence of a complex chromosomal rearrangement. This species is an ideal model for behavioral genomics because the association between genotype and phenotype is absolute, making it possible to identify the genomic bases of phenotypic variation. Findings We initiated a genomic study in this species by characterizing the white-throated sparrow BAC library via filter hybridization with overgo probes designed for the chicken, turkey, and zebra finch. Cross-species hybridization resulted in 640 positive sparrow BACs assigned to 77 chicken loci across almost all macro-and microchromosomes, with a focus on the chromosomes associated with morph. Out of 216 overgos, 36% of the probes hybridized successfully, with an average number of 3.0 positive sparrow BACs per overgo. Conclusions These data will be utilized for determining chromosomal architecture and for fine-scale mapping of candidate genes associated with phenotypic differences. Our research confirms the utility of interspecies hybridization for developing comparative maps in other non-model organisms.

  17. Simplifying resource discovery and access in academic libraries : implementing and evaluating Summon at Huddersfield and Northumbria Universities

    OpenAIRE

    Thoburn, June; Coates, Annette; Stone, Graham

    2010-01-01

    Facilitating information discovery and maximising value for money from library materials is a key driver for academic libraries, which spend substantial sums of money on journal, database and book purchasing. Users are confused by the complexity of our collections and the multiple platforms to access them and are reluctant to spend time learning about individual resources and how to use them - comparing this unfavourably to popular and intuitive search engines like Google. As a...

  18. Intra- and interchromosomal rearrangements between cowpea [Vigna unguiculata (L.) Walp.] and common bean (Phaseolus vulgaris L.) revealed by BAC-FISH.

    Science.gov (United States)

    Vasconcelos, Emanuelle Varão; de Andrade Fonsêca, Artur Fellipe; Pedrosa-Harand, Andrea; de Andrade Bortoleti, Kyria Cilene; Benko-Iseppon, Ana Maria; da Costa, Antônio Félix; Brasileiro-Vidal, Ana Christina

    2015-06-01

    Cowpea (Vigna unguiculata) is an annual legume grown in tropical and subtropical regions, which is economically relevant due to high protein content in dried beans, green pods, and leaves. In this work, a comparative cytogenetic study between V. unguiculata and Phaseolus vulgaris (common bean) was conducted using BAC-FISH. Sequences previously mapped in P. vulgaris chromosomes (Pv) were used as probes in V. unguiculata chromosomes (Vu), contributing to the analysis of macrosynteny between both legumes. Thirty-seven clones from P. vulgaris 'BAT93' BAC library, corresponding to its 11 linkage groups, were hybridized in situ. Several chromosomal rearrangements were identified, such as translocations (between BACs from Pv1 and Pv8; Pv2 and Pv3; as well as Pv2 and Pv11), duplications (BAC from Pv3), as well as paracentric and pericentric inversions (BACs from Pv3, and Pv4, respectively). Two BACs (from Pv2 and Pv7), which hybridized at terminal regions in almost all P. vulgaris chromosomes, showed single-copy signal in Vu. Additionally, 17 BACs showed no signal in V. unguiculata chromosomes. The present results demonstrate the feasibility of using BAC libraries in comparative chromosomal mapping and karyotype evolution studies between Phaseolus and Vigna species, and revealed several macrosynteny and collinearity breaks among both legumes. PMID:25634499

  19. Data Management inside the Library: Assessing Electronic Resources Data Using the Data Asset Framework Methodology

    Science.gov (United States)

    Ogier, Andi; Hall, Monena; Bailey, Annette; Stovall, Connie

    2014-01-01

    Rapidly growing within academic libraries, library data services have often been focused on assessing research trends and building partnerships outside the library. There are distinct benefits, however, to using data audit methodologies created for these external assessments of researcher practices inside the library as well. In this article, we…

  20. "The Open Library at AU" (Athabasca University): Supporting Open Access and Open Educational Resources

    Science.gov (United States)

    Elliott, Colin; Fabbro, Elaine

    2015-01-01

    To address challenges that learners, course creators, librarians and academics involved with OER and MOOCs are facing when looking for scholarly materials, Athabasca University Library has initiated the development of "the Open Library at AU." This open library is a full library website that provides easy access to open and free…

  1. Herpesvirus BACs: Past, Present, and Future

    OpenAIRE

    Charles Warden; Qiyi Tang; Hua Zhu

    2011-01-01

    The herpesviridae are a large family of DNA viruses with large and complicated genomes. Genetic manipulation and the generation of recombinant viruses have been extremely difficult. However, herpesvirus bacterial artificial chromosomes (BACs) that were developed approximately 10 years ago have become useful and powerful genetic tools for generating recombinant viruses to study the biology and pathogenesis of herpesviruses. For example, BAC-directed deletion mutants are commonly used to determ...

  2. A BAC-based physical map of Zhikong scallop (Chlamys farreri Jones et Preston.

    Directory of Open Access Journals (Sweden)

    Xiaojun Zhang

    Full Text Available Zhikong scallop (Chlamys farreri is one of the most economically important aquaculture species in China. Physical maps are crucial tools for genome sequencing, gene mapping and cloning, genetic improvement and selective breeding. In this study, we have developed a genome-wide, BAC-based physical map for the species. A total of 81,408 clones from two BAC libraries of the scallop were fingerprinted using an ABI 3130xl Genetic Analyzer and a fingerprinting kit developed in our laboratory. After data processing, 63,641 (∼5.8× genome coverage fingerprints were validated and used in the physical map assembly. A total of 3,696 contigs were assembled for the physical map. Each contig contained an average of 10.0 clones, with an average physical size of 490 kb. The combined total physical size of all contigs was 1.81 Gb, equivalent to approximately 1.5 fold of the scallop haploid genome. A total of 10,587 BAC end sequences (BESs and 167 markers were integrated into the physical map. We evaluated the physical map by overgo hybridization, BAC-FISH (fluorescence in situ hybridization, contig BAC pool screening and source BAC library screening. The results have provided evidence of the high reliability of the contig physical map. This is the first physical map in mollusc; therefore, it provides an important platform for advanced research of genomics and genetics, and mapping of genes and QTL of economical importance, thus facilitating the genetic improvement and selective breeding of the scallop and other marine molluscs.

  3. CURL: A Searchable CUrricular Resources Library of Earth and Environmental Science Websites

    Science.gov (United States)

    O'Connell, S.; O'Connell, S.; Klassen, T.; Roy, M.; Glynn, M.

    2001-05-01

    A searchable web-based Curricular Resources Library (CURL) has been developed by earth and environmental science faculty from four New England schools (Dartmouth College, Connecticut College, Trinity College, and Wesleyan University) in conjunction with Wesleyan's Information Technology Services (ITS) and librarians. The site (http://www.wesleyan.edu/curl/) provides access to pre-selected earth and environmental science material. CURL sites are identified by faculty who provide information about the site in the following nine fields: Annotation (description), Discipline, Keywords, Origin of Site, Reason for Submission, Submitted By, URL, URL Title, URL Type. Subject Headings conform to those developed by the Library of Congress. These are determined by the Wesleyan University science librarian who enters them into the database. The Curl database can be searched three ways: 1) Browsing alphabetically by Subject Heading (e.g. remote sensing but not satellite pictures), 2) Keyword search which will look for an exact match of a term in any of the designated fields (e.g. URL type, origin of site, discipline), and 3) Advanced search, which allows you to combine terms from any of ten different fields in a single search. Results are displayed in alphabetical order and include the URL title, the URL, and a brief annotation that describes the website. There is an active link to the URL. A web link checker accesses sites to identify broken links. After several unsuccessful attempts, the submitter is contacted and asked to update or delete the site. Between 200 and 250 earth and environmental sites are presently catalogued through CURL. This format could be used by any academic discipline and future plans allow for web resources to span collections.

  4. Charting a Course through CORAL: Texas A&M University Libraries' Experience Implementing an Open-Source Electronic Resources Management System

    Science.gov (United States)

    Hartnett, Eric; Beh, Eugenia; Resnick, Taryn; Ugaz, Ana; Tabacaru, Simona

    2013-01-01

    In 2010, after two previous unsuccessful attempts at electronic resources management system (ERMS) implementation, Texas A&M University (TAMU) Libraries set out once again to find an ERMS that would fit its needs. After surveying the field, TAMU Libraries selected the University of Notre Dame Hesburgh Libraries-developed, open-source ERMS, CORAL…

  5. Public Library Clients Prefer Formal Classes for Initial Training on Library’s Online Resources and Informal, On-Demand Assistance for Further Training. A Review of: Ruthven, J. (2010. Training needs and preferences of adult public library clients in the use of online resources. The Australian Library Journal, 59(3, 108-117.

    Directory of Open Access Journals (Sweden)

    Diana K. Wakimoto

    2012-03-01

    Full Text Available Objective – To discover public library clients’ needs and preferences for modes of training on the use of the Internet and the libraries’ online resources and to apply these findings to improve training offered by public library staff.Design – Multiple exploratory case study.Setting – Two public libraries in New South Wales, Australia: a regional library (Mudgee Branch of the Mid-Western Regional Council Library Service and a metropolitan library (Marrickville Central Library.Subjects – A total of 24 public library clients. The participants were split evenly between the two libraries, with 12 from the Mudgee Branch and 12 from the Marrickville Central. The respondents were further subdivided into two groups based on age (35 to 44 years old and 65 or older and evenly distributed by sex within the groups.Methods – This study used naturalistic inquiry to frame the multiple exploratory case study of two public libraries. Ruthven used maximum variation sampling to guide the selection of participants. Library staff helped the researcher to identify possible participants at Marrickville, while the researcher advertised for participants at Mudgee Library and at an Internet/database course taught at the Mudgee Business Enterprise Centre. She used snowball sampling to find additional participants at both sites. Ruthven conducted semi-structured interviews with the participants, with questions covering their preferences, recommendations, and needs for online resource training. The data from the interviews and search logs were analyzed using inductive data analysis.Main Results – Participants preferred small group, face-to-face, formalized instruction for initial training on online resources. For further training, participants preferred individualized assistance and immediate support instead of formal classes. They noted a lack of training opportunities and a lack of help from library staff as sources of frustration when trying to learn to

  6. Use and Cost of Electronic Resources in Central Library of Ferdowsi University Based on E-metrics

    OpenAIRE

    Mohammad Reza Davarpanah; Nayereh Dadkhah

    2012-01-01

    The purpose of this study was to investigate the usage of electronic journals in Ferdowsi University, Iran based on e-metrics. The paper also aimed to emphasize the analysis of cost-benefit and the correlation between the journal impact factors and the usage data. In this study experiences of Ferdowsi University library on licensing and usage of electronic resources was evaluated by providing a cost-benefit analysis based on the cost and usage statistics of electronic resources. Vendor-provid...

  7. Discussion on Popularizing University Library Information Resources%论高校图书馆信息资源宣传推广工作

    Institute of Scientific and Technical Information of China (English)

    蔡瑞

    2012-01-01

    This paper expounds the importance of popularizing university library information resources and analyzes the factors which influence the popularization and use of the library of resources from the perspectives of users and library.Then the author gives some suggestions to popularizing college library resources.%本文阐述了高校图书馆信息资源推广的重要性,从用户方面和图书馆方面分析了制约资源推广利用的因素,对高校图书馆的资源推广服务提出建议。

  8. The Role of Free Internet Resources for Library Technical Services and Reference in a Hungarian LIS Continuing Education Course

    Science.gov (United States)

    Koltay, Tibor

    2006-01-01

    After providing background to Continuing Professional Education (CPE) of librarians in Hungary, this article outlines the content of a course that seeks to make Hungarian librarians familiar with free Internet resources that are usable for library technical services and reference. One of the most important topics covered in the course is the…

  9. The Availability and Utilization of School Library Resources in Some Selected Secondary Schools (High School) in Rivers State

    Science.gov (United States)

    Owate, C. N.; Iroha, Okpa

    2013-01-01

    This study investigates the availability and utilization of school library resources by Secondary School (High School) Students. Eight Selected Secondary Schools in Rivers State, Nigeria were chosen based on their performance in external examinations and geographic locations. In carrying out the research, questionnaires were administered to both…

  10. Impact of Electronic Resources and Usage in Academic Libraries in Ghana: Evidence from Koforidua Polytechnic & All Nations University College, Ghana

    Science.gov (United States)

    Akussah, Maxwell; Asante, Edward; Adu-Sarkodee, Rosemary

    2015-01-01

    The study investigates the relationship between impact of electronic resources and its usage in academic libraries in Ghana: evidence from Koforidua Polytechnic & All Nations University College, Ghana. The study was a quantitative approach using questionnaire to gather data and information. A valid response rate of 58.5% was assumed. SPSS…

  11. How Can Libraries Determine the Value in Collecting, Managing, Preserving, and/or Cataloging E-Resources?

    Science.gov (United States)

    King, Douglas

    2009-01-01

    The author recently asked his colleagues, "How can libraries determine the value in collecting, managing, preserving, and/or cataloging e-resources?" hoping to challenge readers to come up with some thoughtful opinions and useful tips. Not surprisingly, he received a handful of substantially different but uniformly provocative and creative…

  12. Extending Geant4 Parallelism with External Libraries (MPI, TBB) and Its Use on HPC Resources

    CERN Document Server

    Dotti, Andrea; Barrand, Guy; Hrivnacova, Ivana; Murakami, Koichi

    2016-01-01

    With Geant4 Version 10.0, released in December 2013, one of the most widely used Monte-Carlo codes has been ported to take full advantage of multi- and many-core CPUs thanks to the introduction of event-level parallelism via multithreading. In this paper we review recent developments to allow for a better integration of parallel Geant4 jobs with external libraries. We have chosen to develop examples using the popular Intel Threading Building Block (for short TBB) as an alternative parallelization approach to the native Geant4 POSIX. To simplify the scaling of a Geant4 application across nodes on a cluster we are improving the support of MPI in Geant4. In particular it is now possible to run an hybrid MPI/MT application that uses MPI to scale across nodes and MT to scale across cores. %The recent developments allow users to easily implement parallel application resources that scale on a very large number of nodes and cores typical of HPC resources.

  13. 454 sequencing of pooled BAC clones on chromosome 3H of barley

    Directory of Open Access Journals (Sweden)

    Yamaji Nami

    2011-05-01

    Full Text Available Abstract Background Genome sequencing of barley has been delayed due to its large genome size (ca. 5,000Mbp. Among the fast sequencing systems, 454 liquid phase pyrosequencing provides the longest reads and is the most promising method for BAC clones. Here we report the results of pooled sequencing of BAC clones selected with ESTs genetically mapped to chromosome 3H. Results We sequenced pooled barley BAC clones using a 454 parallel genome sequencer. A PCR screening system based on primer sets derived from genetically mapped ESTs on chromosome 3H was used for clone selection in a BAC library developed from cultivar "Haruna Nijo". The DNA samples of 10 or 20 BAC clones were pooled and used for shotgun library development. The homology between contig sequences generated in each pooled library and mapped EST sequences was studied. The number of contigs assigned on chromosome 3H was 372. Their lengths ranged from 1,230 bp to 58,322 bp with an average 14,891 bp. Of these contigs, 240 showed homology and colinearity with the genome sequence of rice chromosome 1. A contig annotation browser supplemented with query search by unique sequence or genetic map position was developed. The identified contigs can be annotated with barley cDNAs and reference sequences on the browser. Homology analysis of these contigs with rice genes indicated that 1,239 rice genes can be assigned to barley contigs by the simple comparison of sequence lengths in both species. Of these genes, 492 are assigned to rice chromosome 1. Conclusions We demonstrate the efficiency of sequencing gene rich regions from barley chromosome 3H, with special reference to syntenic relationships with rice chromosome 1.

  14. A Comparative Analysis of the Availability of Information Resources on Ibibio Culture in the University of Uyo and Akwa Ibom State Public Library

    Science.gov (United States)

    Okon, Henry Itohowo; Simon, Jehu S.; Akai, Iniobong

    2015-01-01

    This study reports the results of a survey of the available holdings of information resources on Ibibio culture in the University of Uyo Library and Akwa Ibom State Library. The specific objectives of the study were to determine the different size of information resources on funeral, fattening (Mbobo), taboos, myths as well as dissemination in the…

  15. Selected indicators of the range and use of printed resources in Polish academic libraries

    OpenAIRE

    Górny, Mirosław; Jazdon, Artur; Nowak, Piotr

    1999-01-01

    Reports the results of a survey rating the amount and degree of the utilisation of printed materials in Polish academic libraries. The results which are presented by type (for example chemical, biological, technical libraries etc.) as well as by function (research libraries and libraries of educational institutions), concern the number of materials, including both books and periodicals. The authors include the amount of material in English as well as in other foreign l...

  16. Constructing library networks with Chinese characteristics: bringing about society-wide sharing of information resources

    OpenAIRE

    Salman Nazari

    2006-01-01

    As one of the most important components of a nations basic facilities, the networking of the library plays a vital role in terms of promoting the sharing of access to information. Although the traditional methods of literature indexing and accessing will continue to exist and develop, library networking will become the new model and the direction for the development of the library. Through the discussion of the current situation of information sharing in the Chinese library, the author analyz...

  17. GeoDeepDive: Towards a Machine Reading-Ready Digital Library and Information Integration Resource

    Science.gov (United States)

    Husson, J. M.; Peters, S. E.; Livny, M.; Ross, I.

    2015-12-01

    Recent developments in machine reading and learning approaches to text and data mining hold considerable promise for accelerating the pace and quality of literature-based data synthesis, but these advances have outpaced even basic levels of access to the published literature. For many geoscience domains, particularly those based on physical samples and field-based descriptions, this limitation is significant. Here we describe a general infrastructure to support published literature-based machine reading and learning approaches to information integration and knowledge base creation. This infrastructure supports rate-controlled automated fetching of original documents, along with full bibliographic citation metadata, from remote servers, the secure storage of original documents, and the utilization of considerable high-throughput computing resources for the pre-processing of these documents by optical character recognition, natural language parsing, and other document annotation and parsing software tools. New tools and versions of existing tools can be automatically deployed against original documents when they are made available. The products of these tools (text/XML files) are managed by MongoDB and are available for use in data extraction applications. Basic search and discovery functionality is provided by ElasticSearch, which is used to identify documents of potential relevance to a given data extraction task. Relevant files derived from the original documents are then combined into basic starting points for application building; these starting points are kept up-to-date as new relevant documents are incorporated into the digital library. Currently, our digital library stores contains more than 360K documents supplied by Elsevier and the USGS and we are actively seeking additional content providers. By focusing on building a dependable infrastructure to support the retrieval, storage, and pre-processing of published content, we are establishing a foundation for

  18. Electronic Resource Expenditure and the Decline in Reference Transaction Statistics in Academic Libraries

    Science.gov (United States)

    Dubnjakovic, Ana

    2012-01-01

    The current study investigates factors influencing increase in reference transactions in a typical week in academic libraries across the United States of America. Employing multiple regression analysis and general linear modeling, variables of interest from the "Academic Library Survey (ALS) 2006" survey (sample size 3960 academic libraries) were…

  19. Not an Inexhaustible Resource: Valuation and Depreciation of Library Collections in the Queensland Department of Education.

    Science.gov (United States)

    Cram, Jennifer

    1997-01-01

    Anecdotal evidence suggests that valuation of library collections is not being addressed by library managers, despite the growing popularity of accrual accounting in publicly funded institutions. This article discusses the implications of asset valuation and describes the development and implementation a method for valuing the library collections…

  20. Preference and Use of Electronic Information and Resources by Blind/Visually Impaired in NCR Libraries in India

    Directory of Open Access Journals (Sweden)

    Shailendra Kumar

    2013-06-01

    Full Text Available This paper aims to determine the preference and use of electronic information and resources by blind/visually impaired users in the leading National Capital Region (NCR libraries of India. Survey methodology has been used as the basic research tool for data collection with the help of questionnaires. The 125 in total users surveyed in all the five libraries were selected randomly on the basis of willingness of the users with experience of working in digital environments to participate in the survey. The survey results were tabulated and analyzed with descriptive statistics methods using Excel software and 'Stata version 11'. The findings reveal that ICT have a positive impact in the lives of people with disabilities as it helps them to work independently and increases the level of confidence among them. The Internet is the most preferred medium of access to information among the majority of blind/visually impaired users. The 'Complexity of content available on the net' is found as the major challenge faced during Internet use by blind users of NCR libraries. 'Audio books on CDs/DVDs and DAISY books' are the most preferred electronic resources among the majority of blind/visually impaired users. This study will help the library professionals and organizations/institutions serving people with disabilities to develop effective library services for blind/visually impaired users in the digital environment on the basis of findings on information usage behavior in the study.

  1. THE NSF National Science, Technology, Engineering, and Mathematics Education Digital Library (NSDL) Program; Software for Building a Full-Featured Discipline-Based Web Portal; A Metadata Framework Developed at the Tsinghua University Library To Aid in the Preservation of Digital Resources; A Scalable Architecture for Harvest-Based Digital Libraries; The Design and Evaluation of Interactivities in a Digital Library.

    Science.gov (United States)

    Zia, Lee L.; Almasy, Edward; Sleasman, David; Bower, Rachael; Niu, Jingfang; Liu, Xiaoming; Maly, Kurt; Zubair, Mohammad; Nelson, Michael L.; Brody, Tim; Harnard, Stevan; Carr, Les; Budhu, Muniram; Coleman, Anita

    2002-01-01

    Includes five articles that discuss digital library programs related to the National Science Foundation; software for building Web portals; metadata developed at Tsinghua University Library (China) for preserving digital resources; the Open Archives Initiative for metadata and the need for a common infrastructure; and interactivity in a digital…

  2. A hybrid BAC physical map of potato: a framework for sequencing a heterozygous genome

    Directory of Open Access Journals (Sweden)

    de Boer Jan M

    2011-12-01

    Full Text Available Abstract Background Potato is the world's third most important food crop, yet cultivar improvement and genomic research in general remain difficult because of the heterozygous and tetraploid nature of its genome. The development of physical map resources that can facilitate genomic analyses in potato has so far been very limited. Here we present the methods of construction and the general statistics of the first two genome-wide BAC physical maps of potato, which were made from the heterozygous diploid clone RH89-039-16 (RH. Results First, a gel electrophoresis-based physical map was made by AFLP fingerprinting of 64478 BAC clones, which were aligned into 4150 contigs with an estimated total length of 1361 Mb. Screening of BAC pools, followed by the KeyMaps in silico anchoring procedure, identified 1725 AFLP markers in the physical map, and 1252 BAC contigs were anchored the ultradense potato genetic map. A second, sequence-tag-based physical map was constructed from 65919 whole genome profiling (WGP BAC fingerprints and these were aligned into 3601 BAC contigs spanning 1396 Mb. The 39733 BAC clones that overlap between both physical maps provided anchors to 1127 contigs in the WGP physical map, and reduced the number of contigs to around 2800 in each map separately. Both physical maps were 1.64 times longer than the 850 Mb potato genome. Genome heterozygosity and incomplete merging of BAC contigs are two factors that can explain this map inflation. The contig information of both physical maps was united in a single table that describes hybrid potato physical map. Conclusions The AFLP physical map has already been used by the Potato Genome Sequencing Consortium for sequencing 10% of the heterozygous genome of clone RH on a BAC-by-BAC basis. By layering a new WGP physical map on top of the AFLP physical map, a genetically anchored genome-wide framework of 322434 sequence tags has been created. This reference framework can be used for anchoring and

  3. GENSAT BAC Cre-recombinase driver lines to study the functional organization of cerebral cortical and basal ganglia circuits

    OpenAIRE

    Gerfen, Charles R.; Paletzki, Ronald; Heintz, Nathaniel

    2013-01-01

    Recent development of molecular genetic techniques are rapidly advancing understanding of the functional role of brain circuits in behavior. Critical to this approach is the ability to target specific neuron populations and circuits. The collection of over 250 BAC Cre-recombinase driver lines produced by the GENSAT project provides a resource for such studies. Here we provide characterization of GENSAT BAC-Cre driver lines with expression in specific neuroanatomical pathways within the cerebr...

  4. Libraries - Iowa DNR - NRGIS Library

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The Natural Resources Geographic Information System (NRGIS) Library is a Geographic Information System (GIS) repository developed and maintained by the GIS Section...

  5. Construction of the Bac-to-Bac System of Bombyx mori Nucleopolyhedroviru

    Institute of Scientific and Technical Information of China (English)

    Jin-shan HUANG; Bi-fang HAO; Xiu-lian SUN; Fei DENG; Hua-lin WANG; Zhi-hong HU

    2007-01-01

    To construct the Bac-to-Bac expression system of Bombyx mori nucleopolyhedrovirus (BmNPV), a transfer vector was constructed which contained an Escherichia coli (E. coli) mini-F replicon and a lacZ: attTN7: lacZ cassette within the upstream and downstream regions of the BmNPV polyhedrin gene. B. mori larvae were cotransfected with wild-type BmNPV genomic DNA and the transfer vector through subcutaneous injection to generate recombinant viruses by homologous recombination in vivo. The genomic DNA of budded viruses extracted from the hemolymph of the transfected larvae was used to transform E. coli DH10B. Recombinant bacmids were screened by kanamycin resistance, PCR and restriction enzyme (REN) digestion. One of the bacmid colonies, BmBacJS13, which had similar REN profiles to that of wild-type BmNPV, was selected for further research. To investigate the infectivity of BmBacJS13, the polyhedrin gene was introduced into the bacmid and the resultant recombinant (BmBacJS13-ph) was transfected to BmN cells. The budded viruses were collected from the supernatant of the transfected cells and used for infecting BmN cells. Growth curve analysis indicated that BmBacJS13-ph had a similar growth curve to that of wild-type BmNPV. Bio-assays indicated that BmBacJS13-ph was also infectious to B. mori larvae.

  6. Development of the BAC Physical Maps of Wheat Chromosome 6B for Its Genomic Sequencing

    OpenAIRE

    Kobayashi, A.; Katagiri, S.; Karasawa, W.; Takumi, S.; Doležel, J. (Jaroslav); Ogihara, Y.; Handa, H.

    2015-01-01

    For a purpose of better understanding the genome structure of wheat and accelerating the development of DNA markers for gene isolations and breeding, the Japanese research group, as a member of The International Wheat Genome Sequencing Consortium, is now conducting the physical mapping and genomic sequencing of wheat chromosome 6B of ‘Chinese Spring’ (CS). BAC libraries were constructed respectively using the short and long arm-specific DNAs extracted from the flow-sorted chromosome 6BS and 6...

  7. Marketing the Virtual Library

    Science.gov (United States)

    Fagan, Jody Condit

    2009-01-01

    Far more people are familiar with their local public or college library facility than their library's website and online resources. In fact, according to a recent survey, 96% of Americans said they had visited a library in person, but less than one-third have visited their online library. Since everyone agrees that online library resources are…

  8. Defining an open access resource strategy for research libraries:Part Ⅱ——Challenges and responses

    Institute of Scientific and Technical Information of China (English)

    Xiaolin; ZHANG; Yan; ZENG; Lin; LI; Xiwen; LIU

    2012-01-01

    Purpose:This paper aims to provide a systematic analysis of challenges and responses of the imminent open access-based environment so as to enable sound development of open access(OA)resource strategies.Design/methodology/approach:A systematic and evidence-based analysis is used to discover,identify,and contextualize the factors that relate or interact.A challenge metric for the overall context and additional frameworks or alternative lists is used for the detailed analysis.Findings:Research libraries are facing a reality conflict between the traditional library solution and the OA solution.New resource strategies are needed but have to be based on an open knowledge infrastructure and a systematic analysis of factors and alternatives.Support for OA publishing is becoming a major task and complicated capabilities involved call for libraries to play a central role.Hybrid collection development should be in place to take full advantages of the open world while ensuring reliable and trusted retrieval,reuse,and preservation.Research limitations:The research covers main developments in US and European countries mostly in scientific fields and for scholarly papers.Practical implications:There are already evidences for sound OA resource policies.With the imminent dominance of OA resources,research libraries should and can set up their OA resource strategies.Originality/value:The paper suggests a factor metric as an analysis framework,presents rationale lists and policies to support OA publishing,and proposes a hybrid collection strategy to plan for collection development,retrieval,and preservation.

  9. Physical mapping in large genomes: accelerating anchoring of BAC contigs to genetic maps through in silico analysis.

    Science.gov (United States)

    Paux, Etienne; Legeai, Fabrice; Guilhot, Nicolas; Adam-Blondon, Anne-Françoise; Alaux, Michaël; Salse, Jérôme; Sourdille, Pierre; Leroy, Philippe; Feuillet, Catherine

    2008-02-01

    Anchored physical maps represent essential frameworks for map-based cloning, comparative genomics studies, and genome sequencing projects. High throughput anchoring can be achieved by polymerase chain reaction (PCR) screening of bacterial artificial chromosome (BAC) library pools with molecular markers. However, for large genomes such as wheat, the development of high dimension pools and the number of reactions that need to be performed can be extremely large making the screening laborious and costly. To improve the cost efficiency of anchoring in such large genomes, we have developed a new software named Elephant (electronic physical map anchoring tool) that combines BAC contig information generated by FingerPrinted Contig with results of BAC library pools screening to identify BAC addresses with a minimal amount of PCR reactions. Elephant was evaluated during the construction of a physical map of chromosome 3B of hexaploid wheat. Results show that a one dimensional pool screening can be sufficient to anchor a BAC contig while reducing the number of PCR by 384-fold thereby demonstrating that Elephant is an efficient and cost-effective tool to support physical mapping in large genomes. PMID:18038165

  10. Innovation on hospital library human resources management%医院图书馆人力资源管理创新

    Institute of Scientific and Technical Information of China (English)

    王玉双

    2014-01-01

    Based on the problems which can described as backward management of human resources, librarian quality, brain drain and irrational human resources structure of human resources management in hospital library, new concept of hospital library human resources management is presented. Suggestions such as implementing scientific human resources management and training, post appointment and performance system and making human resources management policy are also discussed.%结合制约医院图书馆发展中人力资源管理的管理相对滞后、馆员本身的问题、人才流失严重、人才结构不合理等因素,提出了医院图书馆人力资源管理的新理念,阐述了实施科学人才管理、人才培训、队伍建设以及建立绩效考核制度、岗位聘用制度、人才政策体系等建议。

  11. News from the Library: Online particle physics information: a unique compilation of information resources in particle physics

    CERN Multimedia

    CERN Library

    2012-01-01

    Are you looking for some specific information in particle physics? For example, the main literature databases, data repositories or laboratories...   Just go to the Libary's Online Particle Physics Information page. There you'll find a wide selection of relevant information, as well as resources in particle physics and related areas. The collection covers all aspects of the discipline - in addition to traditional scientific information resources you can find, for example, a selection of relevant blogs and art websites. This webpage is an extended and regularly updated version of the chapter on Online Particle Physics Information in the Review of Particle Properties. It is maintained by the CERN Library team which welcomes suggestions for additions and updates: library.desk@cern.ch.  

  12. Immunotoxin BAC5-CT treated nasopharyngeal carcinoma

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    In this study, immunotoxin (IT) was prepared by conjugating BAC5 and CT with SPDP. The effects of IT on NPC and its mechanisms were explored using double labeled with radioactive nuclides, immunography and electron microscope technique in vivo and in vitro. The specific concentration of BAC5 in the tumor area showed. The radioactivity rate of tumor/nontumor (T/NT) was up to 10.26. IT had cytotoxic effects both on the cultured CNE-2 cell line and tumor multicell spheroides. In vivo, the preliminary result indicated that IT also had a inhibitory action on the nude mice models bearing human NPC (Reported in another article). Under electron microscope, the necrosis and apoptosis of tumor cells were found. The membranes of most tumor cells were found intacted not or corrosined, some of them had the character of apoptosis, including reduce of tumor cells membrane villi, condensation of cytoplasm and pyknosis or cleavage of nuclear. There were many of apoptosis bodies, which were occasionally phagocytosed by tumor cells. The infiltration of immunocytoes in tumor tissue could be seen. The results indicated that BAC5 can specifically combine with NPC cells and BAC5-CT has the inhibitory effect on NPC in vitro and in vivo, mechanism of which may be related to the effects that ‘warhead' CT dissolute the membrane of tumor cells directly, or/and IT promote the infiltration of immunocytoes so as to induce the apoptosis of tumor cell.

  13. Toolkit Approach to Integrating Library Resources into the Learning Management System

    Science.gov (United States)

    Black, Elizabeth L.

    2008-01-01

    As use of learning management systems (LMS) increases, it is essential that librarians are there. Ohio State University Libraries took a toolkit approach to integrate library content in the LMS to facilitate creative and flexible interactions between librarians, students and faculty in Ohio State University's large and decentralized academic…

  14. Wikipedia Lover, Not a Hater: Harnessing Wikipedia to Increase the Discoverability of Library Resources

    Science.gov (United States)

    Elder, Danielle; Westbrook, R. Niccole; Reilly, Michele

    2012-01-01

    During the spring of 2010, the University of Houston Libraries Digital Services Department began an initiative to promote existing and upcoming collections in the University of Houston Digital Library and drive traffic to the online repository. Spurred by an OCLC report (De Rosa et al. 2005) that only two percent of college and university students…

  15. Factors Influencing Virtual Patron Satisfaction with Online Library Resources and Services

    Science.gov (United States)

    Tyler, Katherine; Hastings, Nancy B.

    2011-01-01

    College students are accessing virtual libraries whether they are on campus or learning from a distance. Academic institutions serving virtual patrons must remain focused on meeting the needs of those library users by continually examining their preferences, their searching behavior, and the information they seek. The purpose of this research was…

  16. Equalizing Access to Electronic Networked Resources: A Model for Rural Libraries in the United States.

    Science.gov (United States)

    Senkevitch, Judith J.; Wolfram, Dietmar

    1994-01-01

    Provides an overview of the current state of networking technology in rural libraries and describes a model for educating rural librarians in accessing electronic networks. Topics discussed include information needs in rural libraries; telecommunications technology access in rural areas; and examples of services to enhance information access.…

  17. A Century of Change: The Evolution of School Library Resources, 1915-2015

    Science.gov (United States)

    Lamb, Annette

    2015-01-01

    School libraries have been in existence since at least the eighth century. However, it wasn't until the twentieth century that the school library was seen primarily as "a source of enrichment for the curriculum, and a means of developing reading and study habits in the pupils" (Clyde 1981, 263). While the formats available and tools for…

  18. Redefining the Facilities of Community College Libraries and Learning Resource Centers in the 21st Century

    Science.gov (United States)

    Simon, Matthew J.

    2004-01-01

    The decision to build a new or renovate an existing community college library has been triggered by one or more of these problems: collection growth, increased student population, or new technologies. Increasingly, the perception of diminished use is triggering reconfiguration of library space. This article discusses implications, case studies,…

  19. Comparative physical maps derived from BAC end sequences of tilapia (Oreochromis niloticus

    Directory of Open Access Journals (Sweden)

    Lindblad-Toh Kerstin

    2010-11-01

    Full Text Available Abstract Background The Nile tilapia is the second most important fish in aquaculture. It is an excellent laboratory model, and is closely related to the African lake cichlids famous for their rapid rates of speciation. A suite of genomic resources has been developed for this species, including genetic maps and ESTs. Here we analyze BAC end-sequences to develop comparative physical maps, and estimate the number of genome rearrangements, between tilapia and other model fish species. Results We obtained sequence from one or both ends of 106,259 tilapia BACs. BLAST analysis against the genome assemblies of stickleback, medaka and pufferfish allowed identification of homologies for approximately 25,000 BACs for each species. We calculate that rearrangement breakpoints between tilapia and these species occur about every 3 Mb across the genome. Analysis of 35,000 clones previously assembled into contigs by restriction fingerprints allowed identification of longer-range syntenies. Conclusions Our data suggest that chromosomal evolution in recent teleosts is dominated by alternate loss of gene duplicates, and by intra-chromosomal rearrangements (~one per million years. These physical maps are a useful resource for comparative positional cloning of traits in cichlid fishes. The paired BAC end sequences from these clones will be an important resource for scaffolding forthcoming shotgun sequence assemblies of the tilapia genome.

  20. A first generation BAC-based physical map of the Asian seabass (Lates calcarifer.

    Directory of Open Access Journals (Sweden)

    Jun Hong Xia

    Full Text Available BACKGROUND: The Asian seabass (Lates calcarifer is an important marine foodfish species in Southeast Asia and Australia. Genetic improvement of this species has been achieved to some extent through selective breeding programs since 1990s. Several genomic tools such as DNA markers, a linkage map, cDNA and BAC libraries have been developed to assist selective breeding. A physical map is still lacking, although it is essential for positional cloning of genes located in quantitative trait loci (QTL and assembly of whole genome sequences. METHODOLOGY/PRINCIPAL FINDINGS: A genome-wide physical map of the Asian seabass was constructed by restriction fingerprinting of 38,208 BAC clones with SNaPshot HICF FPC technique. A total of 30,454 were assembled into 2,865 contigs. The physical length of the assembled contigs summed up to 665 Mb. Analyses of some contigs using different methods demonstrated the reliability of the assembly. CONCLUSIONS/SIGNIFICANCE: The present physical map is the first physical map for Asian seabass. This physical map will facilitate the fine mapping of QTL for economically important traits and the positional cloning of genes located in QTL. It will also be useful for the whole genome sequencing and assembly. Detailed information about BAC-contigs and BAC clones are available upon request.

  1. International Conference of Directors of National Libraries on Resource Sharing in Asia and Oceania, Canberra, 1979: Papers from Australasia and Oceania.

    Science.gov (United States)

    Ronnie, Mary; And Others

    1980-01-01

    Describes four library resource sharing projects in (1) New Zealand, (2) Papua New Guinea, (3) Australia, and (4) Fiji. Numerous shared services are discussed, including national bibliographies, publications exchanges, staff exchanges, clearing centers for duplicates, library planning, and national collections. (LLS)

  2. Defining an open access resource strategy for research libraries: Part Ⅰ—The coming main streamlization of open access resources?

    Institute of Scientific and Technical Information of China (English)

    Xiaolin; ZHANG; Lin; LI; Xiwen; LIU; Yan; ZENG

    2011-01-01

    Open access(OA)resources are becoming a main part of scholarly information.The paper summarizes the fast growth of OA journals and OA repositories,the outstanding OA journal impact increase,the rise of mega-OA journals like PLoS ONE,the overwhelming support of research communities to open access of publicly funded research,and the quick building up of OA support structures.These forces work together to push scholarly communications toward a turning point where OA resources occupy a major and increasing share of scholarly research resources and when management of OA resources becomes a strategic,yet unfamiliar,and potentially disruptive task for research libraries.This paper is composed of two parts.The first part will focus on the development of OA resources,while the second part will analyze the challenges in management of OA resources and develop an OA resource strategy exemplified by some of NSL’s initiatives.

  3. Basis for Resource Allocation: Analysis of Operations in a Large Library System

    Science.gov (United States)

    Martin, Gordon P.; West, Martha W.

    1975-01-01

    Reports on the efforts of the California State University and Colleges to develop a series of cost studies to identify and analyze specific library operations as a basis for decision-making among alternatives. (Author/PF)

  4. Genome resource for the Indonesian coelacanth, Latimeria menadoensis.

    Science.gov (United States)

    Danke, Joshua; Miyake, Tsutomu; Powers, Thomas; Schein, Jacqueline; Shin, Heesun; Bosdet, Ian; Erdmann, Mark; Caldwell, Roy; Amemiya, Chris T

    2004-03-01

    We have generated a BAC library from the Indonesian coelacanth, Latimeria menadoensis. This library was generated using genomic DNA of nuclei isolated from heart tissue, and has an average insert size of 171 kb. There are a total of 288 384-well microtiter dishes in the library (110,592 clones) and its genomic representation is estimated to encompass > or = 7X coverage based on the amount of DNA presumably cloned in the library as well as via hybridization with probes to a small set of single copy genes. This genomic resource has been made available to the public and should prove useful to the scientific community for many applications, including comparative genomics, molecular evolution and conservation genetics.

  5. Distribution and uses of legume DNA clone resources

    International Nuclear Information System (INIS)

    Since 1990, my lab has developed and distributed various DNA clone resources for the legumes. In the first several years, the focus was on members of the tropical genus, Vigna, including the widely cultivated species, mungbean (V. radiata) and cowpea (V. unguiculata). Both of these grain legumes play key roles in agriculture in developing countries of Asia (mungbean) and Africa (cowpea). Moreover, because there is substantial genome conservation among legumes, these genetic resources have also been utilized by a wide range of researchers in other crop species. In 1997, my lab began to focus on the development and distribution of a new generation of DNA clone resources; Bacterial Artificial Chromosomes (BAC). A library of these clones was constructed in soybean (Glycine max) the most important legume species worldwide in terms of economic value. Again, the library has become a valuable resource for the legume research community and has been widely used in studies of legume genomics. (author)

  6. 高校复合图书馆信息资源建设探究%Discussion on Information Resources Construction of University Compound Library

    Institute of Scientific and Technical Information of China (English)

    佘小芹

    2012-01-01

    简述复合图书馆的含义及特点,分析影响复合图书馆信息资源建设的因素,提出高校复合图书馆信息资源建设的策略。%This paper simply narrates the meaning and characteristics of compound library,analyses the factors which influence the information resources construction of compound library,and puts forward the information resources construction strategies of university compound library.

  7. 浅谈高校图书馆人力的开发和利用%Discussion on the Development and Utilization of Human Resources in University Library

    Institute of Scientific and Technical Information of China (English)

    李红

    2011-01-01

    本文分析了目前我国图书馆人力资源管理的现状及存在的问题,阐述了图书馆应构建人力资源管理战略,推动图书馆的可持续性发展.%This paper analyzes the current situation of human resource management of library and existing problems, elaborates that the library should build up human resources management strategy and promote the sustainable development of library.

  8. The Analysis on the Construction of Digital Resources in University Library Alliance%高校图书馆联盟数字资源构建解析

    Institute of Scientific and Technical Information of China (English)

    刘雅杰; 果佳欣

    2014-01-01

    阐述了高校图书馆数字资源的建设现状,探讨了高校图书馆联盟构建模式,提出了对高校图书馆联盟数字资源建设的构想。%This paper expounds the current status of information resources of university library, probes into the constructing mode of university library alliance, and puts forward some ideas for the construction of digital resources of university library alliance.

  9. Strategic planning and policy for collection development of e-resources to satisfy user requirements : a case study of J R D Tata Memorial Library

    OpenAIRE

    Venkadesan, S.; Jagannath, Uma; Puttabasavaiah,

    2004-01-01

    The library environment is currently undergoing a rapid and dynamic revolution leading to new generation of libraries with an emphasis on e-resources. On one side, there is an increasing demand for good library collection in terms of large amount of data/information and on the other hand, the publishing media is striving hard to support this demand at lightning speed by way of e-publication as well as online access. As a result, a large number of e-resources are published on all subject areas...

  10. Resources

    Science.gov (United States)

    ... palate - resources Colon cancer - resources Cystic fibrosis - resources Depression - resources Diabetes - resources Digestive disease - resources Drug abuse - resources Eating disorders - resources Elder care - resources Epilepsy - resources Family troubles - ...

  11. Digital case library: a resource for teaching, learning, and diagnosis support in radiology.

    Science.gov (United States)

    Macura, K J; Macura, R T; Morstad, B D

    1995-01-01

    The authors compiled a digital case library, a database of cases for intracranial masses, that can be used as an electronic teaching file and image source for case-based teaching applications, electronic textbooks, and diagnosis support tools. The library is a relational database with a feature-coded image archive that is structured around relevant radiologic findings. Its index for coding image content is structured as a hierarchical image description index that uses the relational format. Rules that control the search direction within the library and that generate lists of diagnostic hypotheses for decision support tools are embedded within the database structure. Currently, the library consists of 200 cases and 1,100 images that present intracranial masses on radiographs, computed tomograms, magnetic resonance images, and angiograms. Each image in the library is indexed according to its radiologic content. The user may search for reference images that contain particular radiologic features by formulating image content-based queries. The hierarchical index of radiologic findings allows multilevel query formulation that depends on the user's level of experience.

  12. The complexity of Rhipicephalus (Boophilus microplus genome characterised through detailed analysis of two BAC clones

    Directory of Open Access Journals (Sweden)

    Valle Manuel

    2011-07-01

    Full Text Available Abstract Background Rhipicephalus (Boophilus microplus (Rmi a major cattle ectoparasite and tick borne disease vector, impacts on animal welfare and industry productivity. In arthropod research there is an absence of a complete Chelicerate genome, which includes ticks, mites, spiders, scorpions and crustaceans. Model arthropod genomes such as Drosophila and Anopheles are too taxonomically distant for a reference in tick genomic sequence analysis. This study focuses on the de-novo assembly of two R. microplus BAC sequences from the understudied R microplus genome. Based on available R. microplus sequenced resources and comparative analysis, tick genomic structure and functional predictions identify complex gene structures and genomic targets expressed during tick-cattle interaction. Results In our BAC analyses we have assembled, using the correct positioning of BAC end sequences and transcript sequences, two challenging genomic regions. Cot DNA fractions compared to the BAC sequences confirmed a highly repetitive BAC sequence BM-012-E08 and a low repetitive BAC sequence BM-005-G14 which was gene rich and contained short interspersed elements (SINEs. Based directly on the BAC and Cot data comparisons, the genome wide frequency of the SINE Ruka element was estimated. Using a conservative approach to the assembly of the highly repetitive BM-012-E08, the sequence was de-convoluted into three repeat units, each unit containing an 18S, 5.8S and 28S ribosomal RNA (rRNA encoding gene sequence (rDNA, related internal transcribed spacer and complex intergenic region. In the low repetitive BM-005-G14, a novel gene complex was found between to 2 genes on the same strand. Nested in the second intron of a large 9 Kb papilin gene was a helicase gene. This helicase overlapped in two exonic regions with the papilin. Both these genes were shown expressed in different tick life stage important in ectoparasite interaction with the host. Tick specific sequence

  13. A Spectrum of Interoperability: The Site for Science Prototype for the NSDL; Re-Inventing the Wheel? Standards, Interoperability and Digital Cultural Content; Preservation Risk Management for Web Resources: Virtual Remote Control in Cornell's Project Prism; Safekeeping: A Cooperative Approach to Building a Digital Preservation Resource; Object Persistence and Availability in Digital Libraries; Illinois Digital Cultural Heritage Community-Collaborative Interactions among Libraries, Museums and Elementary Schools.

    Science.gov (United States)

    Arms, William Y.; Hillmann, Diane; Lagoze, Carl; Krafft, Dean; Marisa, Richard; Saylor, John; Terizzi, Carol; Van de Sompel, Herbert; Gill, Tony; Miller, Paul; Kenney, Anne R.; McGovern, Nancy Y.; Botticelli, Peter; Entlich, Richard; Payette, Sandra; Berthon, Hilary; Thomas, Susan; Webb, Colin; Nelson, Michael L.; Allen, B. Danette; Bennett, Nuala A.; Sandore, Beth; Pianfetti, Evangeline S.

    2002-01-01

    Discusses digital libraries, including interoperability, metadata, and international standards; Web resource preservation efforts at Cornell University; digital preservation at the National Library of Australia; object persistence and availability; collaboration among libraries, museums and elementary schools; Asian digital libraries; and a Web…

  14. 地方高校图书馆信息资源整合探讨%The Investigation of Information Resources Integration In Local University Library

    Institute of Scientific and Technical Information of China (English)

    张扬

    2012-01-01

    Based on the library of Huanggang Normal University, the paper expounds the concept and significance of information resources integration in library, analyzes the necessity of information resources integration in local university library, and on the basis of it discusses the content and strategy of information resources integration in local u- niversity library.%以黄冈师范学院图书馆为例,阐述了图书馆信息资源整合的概念及意义,分析了地方高校图书馆信息资源整合的必要性,并在此基础上探讨地方高校图书馆信息资源整合的内容和具体策略。

  15. Defining an open access resource strategy for research libraries:Part Ⅲ—The strategies and practices of National Science Library

    Institute of Scientific and Technical Information of China (English)

    Xiaolin; ZHANG; Xiwen; LIU; Lin; LI; Yan; ZENG; Li-Ping; KU

    2012-01-01

    Purpose:This paper describes the strategies and practices of National Science Library(NSL),Chinese Academy of Sciences(CAS)in promoting open access(OA)and developing OA resources.Design/methodology/approach:Multi-facet frameworks are devised to guide the development of OA strategies and practices.Key OA initiatives are briefly described along the main aspects of the strategies as they contribute to implementation of the OA strategies.Findings:NSL defined its role as the Chief OA Officer for CAS and a key OA promoter for China.Accordingly,NSL has engaged in multiple fronts of promoting OA,including development of OA strategies for CAS,establishment of itself as an OA knowledge&promotion center,development of the CAS IR system,and support for OA publishing by CAS authors.Research limitations:OA is still evolving,so are the strategies and practices as many actions are experimental and explorative in nature.Open books,open data,and open educational resources are yet to be covered.Comparative studies are needed,so is the evidence-based impact analysis.Practical implications:Institutions can adopt,adapt,or compare with examples and lessons learned are described here.Originality/value:The multi-faceted frameworks,working principles,and lessons learned are based on NSL’s practices which can be valuable to the overall OA development.

  16. Comparative analysis of catfish BAC end sequences with the zebrafish genome

    Directory of Open Access Journals (Sweden)

    Abernathy Jason

    2009-12-01

    Full Text Available Abstract Background Comparative mapping is a powerful tool to transfer genomic information from sequenced genomes to closely related species for which whole genome sequence data are not yet available. However, such an approach is still very limited in catfish, the most important aquaculture species in the United States. This project was initiated to generate additional BAC end sequences and demonstrate their applications in comparative mapping in catfish. Results We reported the generation of 43,000 BAC end sequences and their applications for comparative genome analysis in catfish. Using these and the additional 20,000 existing BAC end sequences as a resource along with linkage mapping and existing physical map, conserved syntenic regions were identified between the catfish and zebrafish genomes. A total of 10,943 catfish BAC end sequences (17.3% had significant BLAST hits to the zebrafish genome (cutoff value ≤ e-5, of which 3,221 were unique gene hits, providing a platform for comparative mapping based on locations of these genes in catfish and zebrafish. Genetic linkage mapping of microsatellites associated with contigs allowed identification of large conserved genomic segments and construction of super scaffolds. Conclusion BAC end sequences and their associated polymorphic markers are great resources for comparative genome analysis in catfish. Highly conserved chromosomal regions were identified to exist between catfish and zebrafish. However, it appears that the level of conservation at local genomic regions are high while a high level of chromosomal shuffling and rearrangements exist between catfish and zebrafish genomes. Orthologous regions established through comparative analysis should facilitate both structural and functional genome analysis in catfish.

  17. Electronic Resources and Mission Creep: Reorganizing the Library for the Twenty-First Century

    Science.gov (United States)

    Stachokas, George

    2009-01-01

    The position of electronic resources librarian was created to serve as a specialist in the negotiation of license agreements for electronic resources, but mission creep has added more functions to the routine work of electronic resources such as cataloging, gathering information for collection development, and technical support. As electronic…

  18. Environmental and Occupational Health Information Resources from the National Library of Medicine

    Institute of Scientific and Technical Information of China (English)

    Philip Wexler

    2004-01-01

    @@ For nearly forty years,the National Library of Medicine' s(NLM)Toxicology and Environmental Health Information Program(TEHIP) has been a signifcant leader in organizing and providing public access to an extensive storehouse of environmental, occupational medicine, and toxicological information through its online databases.

  19. 100 Colleges Sign Up with Google to Speed Access to Library Resources

    Science.gov (United States)

    Young, Jeffrey R.

    2005-01-01

    More than 100 colleges and universities have arranged to give people using the Google Scholar search engine on their campuses more-direct access to library materials. Google Scholar is a free tool that searches scholarly materials on the Web and in academic databases. The new arrangements essentially let Google know which online databases the…

  20. Bringing Vision to Practice: Planning and Provisioning the New Library Resource Center

    Science.gov (United States)

    Wilson, Lisa

    2004-01-01

    The most critical factor in creating a successful school library is the development of a clear vision of the mission and functionality of this integral learning space. However, the process of bringing a vision to realization involves harsh realities and sensible planning. The budget will determine many purchasing decisions and therefore it is…

  1. To improve the sharing rate of library resources in universities%浅议提高高校图书资源共享率

    Institute of Scientific and Technical Information of China (English)

    巴拉

    2013-01-01

      本文分析了目前实现图书资源共享的优势和意义,然后从建立图书馆联盟和实现联合采购、逐步深入推进资源共享和加强信息资源数据库建设等角度谈及了如何做好高校图书资源共享工作。%This paper analyzes the advantages and significance of the realization of library resource sharing, and then talk about how to do the work of library resources sharing of University from the establishment of library alliance and implement joint procurement, gradually promote the sharing of resources and strengthen the construction of information resource database view.

  2. On University Library Resource Sharing in University City%论大学城高校图书馆资源共享

    Institute of Scientific and Technical Information of China (English)

    钟筱莺

    2013-01-01

    大学城高校图书馆的资源共享存在一定的问题,而资源共享是时代发展的必然要求,可通过大学城不同高校图书馆之间资源共享来实现,以扩大不同高校图书馆的读者群,节约办学成本,提高高校办学效率.%In this paper, the present situation of university library resource sharing in university city is discussed. Resource sharing is an inevitable development of the times. It is to be realization by resourcing sharing among different university libraries in the university city and it can expand the readership of different university libraries. Furthermore, resource sharing can reduce the teaching cost and improve teaching efficiency.

  3. Analysis on the Resources Utilization Status in University Library and Countermeasures%高校图书馆资源利用现状分析与对策

    Institute of Scientific and Technical Information of China (English)

    郭竟

    2015-01-01

    揭示了国内高校图书馆资源利用的现状,并针对这种现状与图书馆的自身特点,对如何提高高校图书馆资源使用率提出了具体的对策,重点探讨了资源整合的必要性以及如何进行资源整合。%This paper reveals the status quo of resources utilization in domestic university libraries, and in the light of such a status quo and the features of the libraries their own, puts forward some concrete countermeasures for university libraries to improve the utilization rate of resources, and emphatically probes into the necessity of the resources integration and how to carry out the resources integration.

  4. 高校图书馆的人才资源准备%Preparation for personnel resources of academic libraries

    Institute of Scientific and Technical Information of China (English)

    苏俊彦

    2011-01-01

    文章分析了高校图书馆人才资源的优势以及影响高校图书馆人才发展的不利因素,提出了在人员老龄化、大学生毕业分配就业制度改革下高校图书馆人才资源准备的措施。%This paper analyzes the advantage of academic libraries' personnel resources and the disadvantages of influencing its development, and suggests solutions to preparing for persormel resources for academic libraries under the aging condition and the employment system reform of allocation of the graduates.

  5. Resources Integration Strategies for "985" University Libraries%“985”高校图书馆资源整合策略研究

    Institute of Scientific and Technical Information of China (English)

    沈艳芳; 肖鹏

    2012-01-01

    以11所“985”高校图书馆为样本,通过非介入式调查(包括图书馆实地考察、网站调研、图书馆发现系统调查)对各个图书馆资源整合的现状和问题进行调研,并采取访谈法获取这些高校图书馆领导层对资源整合的策略、态度和看法。对“985”高校图书馆资源整合的系统梳理对我国其他高校图书馆资源整合工作具有很强的指导意义。%Taking eleven "985" university libraries as samples, the authors find out the current status of the integration of library resources and the existing problems by using non-reactive investigation, and then probe their strategies, attitudes and thoughts regarding to integration of library resources by in-depth interviewing with these libraries' leaders. Systematic summary of the integration of library resources has the guiding significance for those university libraries who are working on integration of library resources.

  6. Network Based Educational Environment How Libraries and Librarians Become Organizers of Knowledge Access and Resources

    CERN Document Server

    Pettenati, M C; Pettenati, Corrado

    2000-01-01

    In this paper we will highlight some important issues which will influence the redefinition of roles and duties of libraries and librarians in a networked based educational environment. Although librarians will also keep their traditional roles of faculty support services as well as reference service and research assistance, we identify the participation in the instructional design process, the support in the evaluation, development and use of a proper authoring system and the customization of information access, as being the domains where libraries and librarians should mainly involve themselves in the next future and make profit of their expertise in information and knowledge organization in order to properly and effectively support the institutions in the use of Information Technology in education.

  7. Electronic Resources Management and Long Term Preservation (Is the library a growing organism?)

    OpenAIRE

    Giordano, Tommaso

    2007-01-01

    Digital preservation is a vast and complex issue which involves many aspects and areas of expertise. This paper is rather limited in scope and seeks to develop considerations concerning three questions: the first investigates how the problem of archiving and preservation of electronic journals is perceived by academic libraries, while the second concerns the organizational practices implemented in this area; I conclude with a comparison between the traditional approach and the emerging models...

  8. Research Workshop 3: Search Methodology and Resources, Including Iranian National Library of Medicine

    Directory of Open Access Journals (Sweden)

    F. Karimi PharmD

    2009-01-01

    Full Text Available "nAims: Terminating this workshop, participants are expected to be able to: "nFind the medical databases on Internet; "nBecome familiar with different types of search techniques in medical databases; "nFinding articles in medical databases. "nWorkshop Framework: In this workshop, using Internet, participants will become familiar with search engines and databases abilities, including Iranian National Library of Medicine (INLM, to conduct a search in different items.  

  9. Generation of BAC transgenic epithelial organoids.

    Directory of Open Access Journals (Sweden)

    Gerald Schwank

    Full Text Available Under previously developed culture conditions, mouse and human intestinal epithelia can be cultured and expanded over long periods. These so-called organoids recapitulate the three-dimensional architecture of the gut epithelium, and consist of all major intestinal cell types. One key advantage of these ex vivo cultures is their accessibility to live imaging. So far the establishment of transgenic fluorescent reporter organoids has required the generation of transgenic mice, a laborious and time-consuming process, which cannot be extended to human cultures. Here we present a transfection protocol that enables the generation of recombinant mouse and human reporter organoids using BAC (bacterial artificial chromosome technology.

  10. Targeted isolation, sequence assembly and characterization of two white spruce (Picea glauca BAC clones for terpenoid synthase and cytochrome P450 genes involved in conifer defence reveal insights into a conifer genome

    Directory of Open Access Journals (Sweden)

    Ritland Carol

    2009-08-01

    Full Text Available Abstract Background Conifers are a large group of gymnosperm trees which are separated from the angiosperms by more than 300 million years of independent evolution. Conifer genomes are extremely large and contain considerable amounts of repetitive DNA. Currently, conifer sequence resources exist predominantly as expressed sequence tags (ESTs and full-length (FLcDNAs. There is no genome sequence available for a conifer or any other gymnosperm. Conifer defence-related genes often group into large families with closely related members. The goals of this study are to assess the feasibility of targeted isolation and sequence assembly of conifer BAC clones containing specific genes from two large gene families, and to characterize large segments of genomic DNA sequence for the first time from a conifer. Results We used a PCR-based approach to identify BAC clones for two target genes, a terpene synthase (3-carene synthase; 3CAR and a cytochrome P450 (CYP720B4 from a non-arrayed genomic BAC library of white spruce (Picea glauca. Shotgun genomic fragments isolated from the BAC clones were sequenced to a depth of 15.6- and 16.0-fold coverage, respectively. Assembly and manual curation yielded sequence scaffolds of 172 kbp (3CAR and 94 kbp (CYP720B4 long. Inspection of the genomic sequences revealed the intron-exon structures, the putative promoter regions and putative cis-regulatory elements of these genes. Sequences related to transposable elements (TEs, high complexity repeats and simple repeats were prevalent and comprised approximately 40% of the sequenced genomic DNA. An in silico simulation of the effect of sequencing depth on the quality of the sequence assembly provides direction for future efforts of conifer genome sequencing. Conclusion We report the first targeted cloning, sequencing, assembly, and annotation of large segments of genomic DNA from a conifer. We demonstrate that genomic BAC clones for individual members of multi-member gene

  11. Research on Human Resource Status of Hospital Libraries in Shandong Province%山东省医院图书馆人力资源现状研究

    Institute of Scientific and Technical Information of China (English)

    王今民; 沈小尉; 孙燕

    2009-01-01

    人力资源是医院图书馆赖以生存并可持续发展的主要因素.分析山东省医院图书馆人力资源现状,找出影响医院图书馆人力资源建设的因素,提出医院图书馆应隶属信息科管理、图书馆领导要树立以人为本的人力资源建设理念、确立本馆人力资源建设发展的定位与方向.%Human resources are the principle factor for survival and sustainable development of hospital libraries, the paper analyzes the status of human resources of hospital libraries in Shandong province where it could reveal the factors that effect human resources construction in hospital libraries, and then puts forward that hospital libraries should be under the management of information department,the leader of library should establish people-oriented concept for human resources construction and the developing trends of human resources construction should be established.

  12. High Expression of Insulin-like Growth Factor H (IGF-Ⅱ) Using Bac-to-Bac Expression System

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective In order to obtain mature insulin-like growth factor- Ⅱ ( IGF- Ⅱ ), we used Bac-to-Bac baculovirus expression system. Methods Firstly the IGF- Ⅱ cDNA was cloned into a donor plasmid pFastBac1 and the recombinant pFastBac1 was then introduced into competent cells DH 10Bac. Recombinant bacmids were constructed by transposing a mini-Tn7 element from a donor plasmid pFastBac1 to the mini-attTn7 attachment site on the bacmid where the Tn7 transposition functions were provided in trans by a helper plasmid, and then used to transfect Sf9 insect cells to get recombinant baculovirus. The recombinant baculovirus was used to infect insect cells. Results Agarose gel analysis showed that recombinant donor plasmid pFastBac1 was constructed successfully; Agarose gel analysis of PCR products confirmed recombinant bacmid ; SDS-PAGE and Western Blotting showed that a 7KD protein band appeared. Conclusion The mature IGF- Ⅱ with immunogenecity has been expressed and produced by using Bac-to-Bac expression system.

  13. A dense genetic linkage map for common carp and its integration with a BAC-based physical map.

    Directory of Open Access Journals (Sweden)

    Lan Zhao

    Full Text Available BACKGROUND: Common carp (Cyprinus carpio is one of the most important aquaculture species with an annual global production of 3.4 million metric tons. It is also an important ornamental species as well as an important model species for aquaculture research. To improve the economically important traits of this fish, a number of genomic resources and genetic tools have been developed, including several genetic maps and a bacterial artificial chromosome (BAC-based physical map. However, integrated genetic and physical maps are not available to study quantitative trait loci (QTL and assist with fine mapping, positional cloning and whole genome sequencing and assembly. The objective of this study was to integrate the currently available BAC-based physical and genetic maps. RESULTS: The genetic map was updated with 592 novel markers, including 312 BAC-anchored microsatellites and 130 SNP markers, and contained 1,209 genetic markers on 50 linkage groups, spanning 3,565.9 cM in the common carp genome. An integrated genetic and physical map of the common carp genome was then constructed, which was composed of 463 physical map contigs and 88 single BACs. Combined lengths of the contigs and single BACs covered a physical length of 498.75 Mb, or around 30% of the common carp genome. Comparative analysis between common carp and zebrafish genomes was performed based on the integrated map, providing more insights into the common carp specific whole genome duplication and segmental rearrangements in the genome. CONCLUSION: We integrated a BAC-based physical map to a genetic linkage map of common carp by anchoring BAC-associated genetic markers. The density of the genetic linkage map was significantly increased. The integrated map provides a tool for both genetic and genomic studies of common carp, which will help us to understand the genomic architecture of common carp and facilitate fine mapping and positional cloning of economically important traits for

  14. Discussion on the Resources Integration after Normal University Library Amalgamation%师范院校图书馆合并后资源整合探讨

    Institute of Scientific and Technical Information of China (English)

    李爽

    2012-01-01

    本文从高校图书馆信息管理系统的合并、藏书的剔旧工作、建立并完善“学科馆员”制度等方面,对师范院校图书馆合并后资源整合进行了探讨。%In this paper, the resources integration after normal university library amalgamation is discussed from the following three aspects: the amalgamation of university library information management system, the weeding of library collection, as well as the establishment and perfection of subject librarian system.

  15. The Statistical Analysis on Digital Library Resource Usage -Taking Shanxi University Library as An Example%数字资源使用统计分析——以山西大学图书馆为例

    Institute of Scientific and Technical Information of China (English)

    段惠静

    2012-01-01

    Along with the universities' increasing investment to the library, the reasonable use of library resource becomes more and more important under the digital environment. At the same time, the investment of library digitalized collection' s proportion is getting larger and larger with the network development and the changing methods of scientific research. Thus, the assessing of digital library resource usage is the reflection of the library service a chievements. The article, by taking Shanxi University library as an example, systematically analyzes and explains the statistical data of resource usage related to digital service achievements so as to construct a relatively reasonable digital collection system by fulfilling the requirements of Shanxi University library.%随着高校对图书馆经费投入的不断增加,数字环境下图书馆的合理使用变得越来越重要。而图书馆数字化馆藏的投入也随着网络的发展和人们科研习惯的变迁所占的比重越来越大,因而,对数字资源的使用统计就成为反映数字图书馆服务绩效的重要指标。文章以山西大学图书馆为例,对于数字服务绩效评估相关的使用统计数据进行系统的分析与阐释,借以构建符合山西大学图书馆要求的相对合理的数字馆藏体系。

  16. Extensive conserved synteny of genes between the karyotypes of Manduca sexta and Bombyx mori revealed by BAC-FISH mapping.

    Directory of Open Access Journals (Sweden)

    Yuji Yasukochi

    Full Text Available BACKGROUND: Genome sequencing projects have been completed for several species representing four highly diverged holometabolous insect orders, Diptera, Hymenoptera, Coleoptera, and Lepidoptera. The striking evolutionary diversity of insects argues a need for efficient methods to apply genome information from such models to genetically uncharacterized species. Constructing conserved synteny maps plays a crucial role in this task. Here, we demonstrate the use of fluorescence in situ hybridization with bacterial artificial chromosome probes as a powerful tool for physical mapping of genes and comparative genome analysis in Lepidoptera, which have numerous and morphologically uniform holokinetic chromosomes. METHODOLOGY/PRINCIPAL FINDINGS: We isolated 214 clones containing 159 orthologs of well conserved single-copy genes of a sequenced lepidopteran model, the silkworm, Bombyx mori, from a BAC library of a sphingid with an unexplored genome, the tobacco hornworm, Manduca sexta. We then constructed a BAC-FISH karyotype identifying all 28 chromosomes of M. sexta by mapping 124 loci using the corresponding BAC clones. BAC probes from three M. sexta chromosomes also generated clear signals on the corresponding chromosomes of the convolvulus hawk moth, Agrius convolvuli, which belongs to the same subfamily, Sphinginae, as M. sexta. CONCLUSIONS/SIGNIFICANCE: Comparison of the M. sexta BAC physical map with the linkage map and genome sequence of B. mori pointed to extensive conserved synteny including conserved gene order in most chromosomes. Only a few rearrangements, including three inversions, three translocations, and two fission/fusion events were estimated to have occurred after the divergence of Bombycidae and Sphingidae. These results add to accumulating evidence for the stability of lepidopteran genomes. Generating signals on A. convolvuli chromosomes using heterologous M. sexta probes demonstrated that BAC-FISH with orthologous sequences can be

  17. Libraries for users services in academic libraries

    CERN Document Server

    Alvite, Luisa

    2010-01-01

    This book reviews the quality and evolution of academic library services. It revises service trends offered by academic libraries and the challenge of enhancing traditional ones such as: catalogues, repositories and digital collections, learning resources centres, virtual reference services, information literacy and 2.0 tools.studies the role of the university library in the new educational environment of higher educationrethinks libraries in academic contextredefines roles for academic libraries

  18. Creating a Library of Climate Change Education Resources for Audiences in the Southeast United States

    Science.gov (United States)

    Carroll, J.; McNeal, K. S.; Williams, C. C.; Paz, J. O.; Cho, H. "; Nair, U. S.; Geroux, J.; Guthrie, C.; Wright, K.; Hill, J.

    2011-12-01

    The Climate Literacy Partnership in the Southeast (CLiPSE) is a part of the Climate Change Education Program supported by the National Science Foundation (http://CLiPSE-project.org). The established CLiPSE partnership is dedicated to improving climate literacy in the southeast and promoting scientifically accurate, formal educational resources for the K-12 classroom audience, as well as informal educational resources for audiences such as agriculture, education, leisure, and religious organizations, to name a few. The CLiPSE project has been successful in creating partnerships with the National Geographic Alliances, Departments of Education, and Mississippi Environmental Education Alliance, among others, to determine an effective strategic plan for reaching K-12 audiences. One goal in the strategic plan is to create a catalog of climate change education resources that are aligned to state standards in the SE. Eighty-seven resources from the Climate Literacy and Energy Awareness Network (http://cleanet.org) have been aligned with the state education standards for grades six through twelve in the southeast, beginning with science in Mississippi and expanding to include science and math in the remaining SE states. The criteria for aligning the existing resources includes: matching key terms, topics, and lesson activities with the content strands and essential skills included in the state science framework. By developing a searchable database containing climate resources already aligned with state standards, CLiPSE will have made these resources more appealing to educators in the SE, increasing the likelihood of resources being implemented in the classroom. The CLiPSE Climate Science Team has also created an inventory of scientifically sound, informal resources, which will be available for dispersion to appropriate audiences and communities. Cataloged resources, both formal and informal, grouped by a variety of means, to include audience, grade level, and resource

  19. The Use of Quality Benchmarking in Assessing Web Resources for the Dermatology Virtual Branch Library of the National electronic Library for Health (NeLH)

    OpenAIRE

    Boulos, MN Kamel; Roudsari, AV; Gordon, C.; Gray, JA Muir

    2001-01-01

    Background In 1998, the U.K. National Health Service Information for Health Strategy proposed the implementation of a National electronic Library for Health to provide clinicians, healthcare managers and planners, patients and the public with easy, round the clock access to high quality, up-to-date electronic information on health and healthcare. The Virtual Branch Libraries are among the most important components of the National electronic Library for Health . They aim at creating online kno...

  20. Cloud Storage Mode of University Library Digital Resources%高校图书馆数字资源的云存储模式探讨

    Institute of Scientific and Technical Information of China (English)

    付晓红; 莫筱梅

    2012-01-01

    分析了传统存储模式下高校图书馆数字资源存储存在的问题,结合云存储的优势,讨论了应用云存储模式存储高校图书馆数字资源,提出并分析了两种具体的高校图书馆数字资源云存储模式。%This paper analyzes the problems of the storage of university libraries digital resources under traditional storage mode,discusses the storage of university libraries digital resources which apply cloud storage mode based on the advantage of cloud storage,proposes and analyzes two specific cloud storage modes of university libraries digital resource.

  1. A resource for CERN community: the Bookshop in the Central Library

    CERN Multimedia

    2008-01-01

    If you are looking for technical and scientific books on topics such as IT, Radiation Protection, Detectors and Experimental Techniques, Project Management, or simply for a good read, why not check whether the CERN Bookshop has it in stock? You will be surprised by its coverage in IT (150 titles) and physics (300 titles). In total the Bookshop sells around 500 titles from around 15 publishing houses. However, the offer is not limited to these publishers – the Bookshop staff are ready to order any title that exists in print. In addition, we welcome suggestions from the user community for new titles to be added to the stock. The title list - which is integrated into the CERN library catalogue – is available from http://cdsweb.cern.ch/collection/CERN Bookshop This Bookshop is located in the Central Library, Building 52 1-052 and is open on weekdays from 8.30 a.m. to 5.30 p.m. It can be contacted by e-mail (bookshop@cern.ch). CERN us...

  2. Sequencing of a QTL-rich region of the Theobroma cacao genome using pooled BACs and the identification of trait specific candidate genes

    Directory of Open Access Journals (Sweden)

    Blackmon Barbara P

    2011-07-01

    Full Text Available Abstract Background BAC-based physical maps provide for sequencing across an entire genome or a selected sub-genomic region of biological interest. Such a region can be approached with next-generation whole-genome sequencing and assembly as if it were an independent small genome. Using the minimum tiling path as a guide, specific BAC clones representing the prioritized genomic interval are selected, pooled, and used to prepare a sequencing library. Results This pooled BAC approach was taken to sequence and assemble a QTL-rich region, of ~3 Mbp and represented by twenty-seven BACs, on linkage group 5 of the Theobroma cacao cv. Matina 1-6 genome. Using various mixtures of read coverages from paired-end and linear 454 libraries, multiple assemblies of varied quality were generated. Quality was assessed by comparing the assembly of 454 reads with a subset of ten BACs individually sequenced and assembled using Sanger reads. A mixture of reads optimal for assembly was identified. We found, furthermore, that a quality assembly suitable for serving as a reference genome template could be obtained even with a reduced depth of sequencing coverage. Annotation of the resulting assembly revealed several genes potentially responsible for three T. cacao traits: black pod disease resistance, bean shape index, and pod weight. Conclusions Our results, as with other pooled BAC sequencing reports, suggest that pooling portions of a minimum tiling path derived from a BAC-based physical map is an effective method to target sub-genomic regions for sequencing. While we focused on a single QTL region, other QTL regions of importance could be similarly sequenced allowing for biological discovery to take place before a high quality whole-genome assembly is completed.

  3. Status and Problems of Information Resources Construction in University Libraries%高校图书馆信息资源建设现状及问题

    Institute of Scientific and Technical Information of China (English)

    邢燕丽

    2012-01-01

    对高校图书馆信息资源的现状及问题进行了分析,着重分析了高校图书馆数字资源的现状及问题,以期对今后的工作提供一定的参考。%Based on university library information resources current situation and problem analysis, focuses on the analysis of university library digital resources current situation and problem, with a view to the future work to provide a certain reference.

  4. Thought on the Foreign Periodicals Resources Construction of University Library%高校图书馆外文期刊资源建设思考

    Institute of Scientific and Technical Information of China (English)

    刘迎春

    2011-01-01

    Foreign periodicals is the important part of literature resources in university library.According to the present situation of foreign periodicals publishing,this paper discusses the foreign periodicals resources construction strategies of university library.%外文期刊是高校图书馆文献资源的重要组成部分。针对目前外文期刊出版现状,探讨了高校图书馆外文期刊资源建设策略。

  5. Probe into Digital Library's Resources Co-construction and Sharing%数字图书馆资源共建共享的探讨

    Institute of Scientific and Technical Information of China (English)

    王雅睿

    2015-01-01

    This paper analyzes the advantages of digital library's resources sharing,and puts forward some strategies for promoting digital library's resources co-construction and sharing, which include the technology strategy, talent strategy, legal strategy and industrial layout strategy.%分析了数字图书馆资源共享的优势,提出了促进数字图书馆资源共建共享的策略,包括技术策略、人才策略、法律策略、产业布局策略.

  6. Survey on User Satisfaction of Electronic Resources in University Libraries%高校图书馆电子资源用户满意度调查

    Institute of Scientific and Technical Information of China (English)

    陈姚竹; 马东

    2011-01-01

    通过分析影响用户对图书馆电子资源利用的因素,以美国顾客满意度指数模型为基础,提出关于各个因素的测量指标,对高校图书馆电子资源用户满意度进行测量。%Through analyzing the influencing factors on utilizing library electronic resources, based on the model of American customer satisfaction index, the paper puts forward corresponding measurement indexes, and then measures the user satisfaction on electronic resources of university library

  7. 数字图书馆资源共建共享的探讨%Probe into Digital Library's Resources Co-construction and Sharing

    Institute of Scientific and Technical Information of China (English)

    王雅睿

    2015-01-01

    This paper analyzes the advantages of digital library's resources sharing,and puts forward some strategies for promoting digital library's resources co-construction and sharing, which include the technology strategy, talent strategy, legal strategy and industrial layout strategy.%分析了数字图书馆资源共享的优势,提出了促进数字图书馆资源共建共享的策略,包括技术策略、人才策略、法律策略、产业布局策略.

  8. Use and Cost of Electronic Resources in Central Library of Ferdowsi University Based on E-metrics

    Directory of Open Access Journals (Sweden)

    Mohammad Reza Davarpanah

    2012-07-01

    Full Text Available The purpose of this study was to investigate the usage of electronic journals in Ferdowsi University, Iran based on e-metrics. The paper also aimed to emphasize the analysis of cost-benefit and the correlation between the journal impact factors and the usage data. In this study experiences of Ferdowsi University library on licensing and usage of electronic resources was evaluated by providing a cost-benefit analysis based on the cost and usage statistics of electronic resources. Vendor-provided data were also compared with local usage data. The usage data were collected by tracking web-based access locally, and by collecting vender-provided usage data. The data sources were one-year of vendor-supplied e-resource usage data such as Ebsco, Elsevier, Proquest, Emerald, Oxford and Springer and local usage data collected from the Ferdowsi university web server. The study found that actual usage values differ for vendor-provided data and local usage data. Elsevier has got the highest usage degree in searches, sessions and downloads. Statistics also showed that a small number of journals satisfy significant amount of use while the majority of journals were used less frequent and some were never used at all. The users preferred the PDF rather than HTML format. The data in subject profile suggested that the provided e-resources were best suited to certain subjects. There was no correlation between IF and electronic journal use. Monitoring the usage of e-resources gained increasing importance for acquisition policy and budget decisions. The article provided information about local metrics for the six surveyed vendors/publishers, e.g. usage trends, requests per package, cost per use as related to the scientific specialty of the university.

  9. A high-throughput strategy for screening of bacterial artificial chromosome libraries and anchoring of clones on a genetic map constructed with single nucleotide polymorphisms

    Directory of Open Access Journals (Sweden)

    Deal Karin R

    2009-01-01

    Full Text Available Abstract Background Current techniques of screening bacterial artificial chromosome (BAC libraries for molecular markers during the construction of physical maps are slow, laborious and often assign multiple BAC contigs to a single locus on a genetic map. These limitations are the principal impediment in the construction of physical maps of large eukaryotic genomes. It is hypothesized that this impediment can be overcome by screening multidimensional pools of BAC clones using the highly parallel Illumina GoldenGate™ assay. Results To test the efficacy of the Golden Gate assay in BAC library screening, multidimensional pools involving 302976 Aegilops tauschii BAC clones were genotyped for the presence/absence of specific gene sequences with multiplexed Illumina GoldenGate oligonucleotide assays previously used to place single nucleotide polymorphisms on an Ae. tauschii genetic map. Of 1384 allele-informative oligonucleotide assays, 87.6% successfully clustered BAC pools into those positive for a BAC clone harboring a specific gene locus and those negative for it. The location of the positive BAC clones within contigs assembled from 199190 fingerprinted Ae. tauschii BAC clones was used to evaluate the precision of anchoring of BAC clones and contigs on the Ae. tauschii genetic map. For 41 (95% assays, positive BAC clones were neighbors in single contigs. Those contigs could be unequivocally assigned to loci on the genetic map. For two (5% assays, positive clones were in two different contigs and the relationships of these contigs to loci on the Ae. tauschii genetic map were equivocal. Screening of BAC libraries with a simple five-dimensional BAC pooling strategy was evaluated and shown to allow direct detection of positive BAC clones without the need for manual deconvolution of BAC clone pools. Conclusion The highly parallel Illumina oligonucleotide assay is shown here to be an efficient tool for screening BAC libraries and a strategy for high

  10. 纸质图书是图书馆的核心资源%Paper Books : Core Resources in Library

    Institute of Scientific and Technical Information of China (English)

    郑丽君

    2012-01-01

    The rise of the electronic books brings certain impact to the paper books,but the impact is far from subversive.Due to the paper books giving the readers the best reading,as well as the advantage of preservation,it is still the core resources of libraries.%电子图书的崛起给纸质图书带来一定的冲击,但这种冲击远没有达到颠覆性的强度。由于纸质图书带给读者最佳的阅读,纸质图书具有保存的优越性,因而纸质图书是图书馆的核心资源。

  11. 区域性高校图书馆联盟资源共享运营模式研究——以豫北地区高校资源共享平台为例%A Study on the Operating Mode of Resource Sharing of Library Coalition in Regional University Library -Take University Resources Sharing Platform in Northern Henan for Example

    Institute of Scientific and Technical Information of China (English)

    张文娟

    2012-01-01

    Library information resources sharing is the important part of modernization of university library . This paper puts forward the ideas and building strategy of resource sharing of library coalition in regional university library based on the analysis of its advantages and problems , for example, the construction of resource sharing platform for universities in Northern Henan.%高校图书馆信息资源共建共享是高校图书馆现代化建设的重要内容,本文以建设豫北地区高校资源共享平台为例,分析了地方区域级高校图书馆联盟的优势与问题,提出了构建地方区域级高校图书馆联盟信息资源共建共享的设想和建设策略。

  12. Strategies on the Information Resource Integration and Utilization of University Library%高校图书馆信息资源整合与利用策略

    Institute of Scientific and Technical Information of China (English)

    桂秀梅; 惠青

    2014-01-01

    文章立足高校信息资源重复、闲置的的现状,对当前高校图书馆信息资源整合与利用的问题进行论述,同时提出了高校图书馆的资源整合与利用服务的相关策略,以期促进高校图书馆信息资源合理建设与充分利用,进一步提升图书馆服务水平。%Based on the status of information resources repetition and idle in university library, the paper discussed the problems of information resource integration and utilization, and at the same time, put forward the strategies about the resource integration and utilization services in university library, so as to promote reasonable construction of information resources and make full use of information resources in university library and further enhance the level of library service.

  13. U.S.-U.S.S.R. Seminar on Access to Library Resources through Technology and Preservation (Washington, D.C., July 5-8, 1988). Papers by U.S.S.R. Participants.

    Science.gov (United States)

    International Research and Exchange Board, New York, NY.

    This document contains 13 papers by Soviet participants in the U.S.-U.S.S.R. Seminar on Access to Library Resources through Technology and Preservation: (1) "Automation of Information-Library Work at Scientific and Technical Libraries of the U.S.S.R." (A. S. Sorokin and V. M. Rostovtsev); (2) "Automated Information Systems for Culture and Art" (A.…

  14. User experiences of evidence-based online resources for health professionals: User testing of The Cochrane Library

    Directory of Open Access Journals (Sweden)

    Glenton Claire

    2008-07-01

    Full Text Available Abstract Background Evidence-based decision making relies on easy access to trustworthy research results. The Cochrane Library is a key source of evidence about the effect of interventions and aims to "promote the accessibility of systematic reviews to anyone wanting to make a decision about health care". We explored how health professionals found, used and experienced The Library, looking at facets of user experience including findability, usability, usefulness, credibility, desirability and value. Methods We carried out 32 one-hour usability tests on participants from Norway and the UK. Participants both browsed freely and attempted to perform individually tailored tasks while "thinking aloud". Sessions were recorded and viewed in real time by researchers. Transcriptions and videos were reviewed by one researcher and one designer. Findings reported here reflect issues receiving a high degree of saturation and that we judge to be critical to the user experience of evidence-based web sites, based on principles for usability heuristics, web guidelines and evidence-based practice. Results Participants had much difficulty locating both the site and its contents. Non-native English speakers were at an extra disadvantage when retrieving relevant documents despite high levels of English-language skills. Many participants displayed feelings of ineptitude, alienation and frustration. Some made serious mistakes in correctly distinguishing between different information types, for instance reviews, review protocols, and individual studies. Although most expressed a high regard for the site's credibility, some later displayed a mistrust of the independence of the information. Others were overconfident, thinking everything on The Cochrane Library site shared the same level of quality approval. Conclusion Paradoxically, The Cochrane Library, established to support easy access to research evidence, has its own problems of accessibility. Health professionals

  15. Research on Experiences of Foreign Library Resource Sharing Service Innovation%国外图书馆资源共享服务创新经验研究

    Institute of Scientific and Technical Information of China (English)

    余若琪

    2015-01-01

    介绍国外尤其是美国图书馆信息资源共享服务创新的概况。认为国外图书馆在创新资源发现与传递、共享工作准则、创新性馆际合作模式等方面积累许多案例和经验,值得我国图书馆借鉴。我国图书馆及图书馆员要通过学习国外经验以提升自身业务水平,也要通过参与国内及国际图书馆组织而努力开展创新性服务实践。%The paper introduces general situation of foreign library, especially American library information resources sharing ser-vice innovation. It thinks that cases and experiences of foreign library on innovation resource discovery and delivery, sharing working norm, innovative interlibrary cooperation model are worth learning by Chinese library. Chinese library and librarian should learn foreign related experience to promote their professional level, and participate in national and international library organization to actively devel-op innovative service practice.

  16. The Digital Edition 2.0 and the Digital Library: Services, not Resources

    NARCIS (Netherlands)

    van Zundert, J.; Boot, P.

    2011-01-01

    The article sketches a number of likely developments in scholarly digital editions and other scholarly digital resources. They will be composites of independent and distributed components, containing multiple media, and subject to permanent change. These trends seriously increase the as yet unsolved

  17. "You Just Type in What You Are Looking for": Undergraduates' Use of Library Resources vs. Wikipedia

    Science.gov (United States)

    Colon-Aguirre, Monica; Fleming-May, Rachel A.

    2012-01-01

    This study presents findings from one-on-one interviews with 21 undergraduate students at a large public research university in the southeastern United States. While the preliminary focus of the study was to be students' opinions about and use of Wikipedia as a resource for course-related research, many of the interviews evolved into discussion…

  18. Open and Editable: Exploring Library Engagement in Open Educational Resource Adoption, Adaptation and Authoring

    Science.gov (United States)

    Walz, Anita R.

    2015-01-01

    Open Educational Resources (OER) have saved students millions of dollars in textbook costs and greatly expanded access to a wide variety of educational materials for countless numbers of students and life-long learners. OER have also saved teachers time and effort by allowing them to reuse, modify, and build on materials developed by other…

  19. Recommendations for protecting National Library of Medicine Computing and Networking Resources

    Energy Technology Data Exchange (ETDEWEB)

    Feingold, R.

    1994-11-01

    Protecting Information Technology (IT) involves a number of interrelated factors. These include mission, available resources, technologies, existing policies and procedures, internal culture, contemporary threats, and strategic enterprise direction. In the face of this formidable list, a structured approach provides cost effective actions that allow the organization to manage its risks. We face fundamental challenges that will persist for at least the next several years. It is difficult if not impossible to precisely quantify risk. IT threats and vulnerabilities change rapidly and continually. Limited organizational resources combined with mission restraints-such as availability and connectivity requirements-will insure that most systems will not be absolutely secure (if such security were even possible). In short, there is no technical (or administrative) {open_quotes}silver bullet.{close_quotes} Protection is employing a stratified series of recommendations, matching protection levels against information sensitivities. Adaptive and flexible risk management is the key to effective protection of IT resources. The cost of the protection must be kept less than the expected loss, and one must take into account that an adversary will not expend more to attack a resource than the value of its compromise to that adversary. Notwithstanding the difficulty if not impossibility to precisely quantify risk, the aforementioned allows us to avoid the trap of choosing a course of action simply because {open_quotes}it`s safer{close_quotes} or ignoring an area because no one had explored its potential risk. Recommendations for protecting IT resources begins with discussing contemporary threats and vulnerabilities, and then procedures from general to specific preventive measures. From a risk management perspective, it is imperative to understand that today, the vast majority of threats are against UNIX hosts connected to the Internet.

  20. The Electronic, Eclectic Library.

    Science.gov (United States)

    Dowlin, Kenneth E.

    1980-01-01

    Utilization of telecommunications and computer technology to increase access to information is identified as a primary goal for public libraries. To further service in the future, libraries should consider database services, online access to library resources, online community conferencing, community databases, network resource sharing,…

  1. 浅谈高校图书馆特色资源的开发与利用%On development and utilization of university library's characteristic resources

    Institute of Scientific and Technical Information of China (English)

    梁永忠

    2012-01-01

      阐述了高校图书馆特殊资源的特征,同时根据高校图书特殊资源的内容提出了若干特色资源开发的意见,并对当前高校图书馆特殊资源的利用方式进行了总结和建议。%  This paper expounds the special resources characteristic, at the same time ,according to the university library special resource content offerred a certain number of characteristic resources development opinion, and the libraries in college on the special resources utilization are summarized and suggestions.

  2. 圖書館事業專欄/Marketing of Electronic Information Resources: A Case of The J.D. Rockefeller Research Library, Egerton University/Nerisa Kamar

    OpenAIRE

    Nerisa Kamar

    2008-01-01

    This paper gives a brief overview of electronic information resources and services offered by The J.D. Rockefeller Research Library at Egerton University and the marketing of these resources. The paper examines the various reasons for marketing electronic information resources, with emphasis on the various, and illustrates marketing strategies used by J.D Rockefeller Research library towards effective utilization of the available resources in supporting research, teaching and learnin...

  3. Utilization of Information and Communication Technology (ICT) Resources and Job Effectiveness among Library Staff in the University of Calabar and Cross River University of Technology, Nigeria

    Science.gov (United States)

    Ntui, Aniebiet Inyang; Inyang, Comfort Linus

    2015-01-01

    This study investigated utilization of Information and Communication Technology (ICT) resources and job effectiveness among library staff in the University of Calabar and Cross River University of Technology, Nigeria. To achieve the purpose of this study, four hypotheses were formulated to guide the study. Ex-post facto research design was adopted…

  4. Scholars' Satisfaction with Digital Library Collection and Gaps in the Provision of Effective Information Resources and Services: A Pakistani Perspective

    Science.gov (United States)

    Khan, Amjid; Ahmed, Shamshad; Masrek, Mohamad Noorman

    2014-01-01

    The study aims to explore the researchers' satisfaction with digital library resources and services and how they improved the research culture in Pakistani universities. A descriptive survey method was employed to achieve objectives of this study. Using stratified random sampling, for this survey we selected 14 public sector universities of…

  5. 醫學圖書館網路資源合作館藏發展 Internet Resources Cooperative Collection Development in Medical Library

    Directory of Open Access Journals (Sweden)

    Shwu-jiun Wang

    1999-06-01

    Full Text Available 無Internet has been well-developed and utilized, collection developments for both periodicals and databases and how to share the resources among libraries should be handled and organized by a national institution, such as National Central Library or Science& Technology Information Center. Then other libraries can take the advantages of Internet to collect and organize the valuable information by topics and subjects. It will not only utilize the virtual resources, save time for reference librarians but also help users to locate the information much easier and faster. As a side benefit, the traffic of Internet will also be decreased. Therefore under the present situation of shortage of manpower, high price of periodicals and databases, how to utilize and organize the resources from the Internet has become a possible way to achieve the purposes of collection developments and sharing resources. This article will present few models of medical libraries in the U.S. and European countries as our reference.

  6. 日本图书馆的动漫资源建设特点%Characteristics of Comic Resources Construction in Japanese Libraries

    Institute of Scientific and Technical Information of China (English)

    黄如花; 李英子

    2015-01-01

    文章就日本图书馆的动漫资源建设特点进行了分析,发现日本几乎所有的图书馆均收藏动漫资源,在全国拥有一定数量的漫画专业图书馆,图书馆积极争取社会援助与民间资源,但也存在这缺乏完善的资源收集标准、动漫资源长期保存等难题与问题。%The paper analyses the characteristics of comic resources construction of Japanese libraries. Most of Japanese libraries collect comic resources;There is a certain amount of manga libraries;Libraries try to get social assistance and public resources;There is no comprehensive resourcecollectionstandards;There are problems for long-term preservation of comic resources.

  7. Making Higher Education More Affordable, One Course Reading at a Time: Academic Libraries as Key Advocates for Open Access Textbooks and Educational Resources

    Science.gov (United States)

    Okamoto, Karen

    2013-01-01

    Open access textbooks (OATs) and educational resources (OERs) are being lauded as a viable alternative to costly print textbooks. Some academic libraries are joining the OER movement by creating guides to open repositories. Others are promoting OATs and OERs, reviewing them, and even helping to create them. This article analyzes how academic…

  8. 云存储模式下图书馆数字资源建设%Library Digital Resources Construction Based on Cloud Storage Mode

    Institute of Scientific and Technical Information of China (English)

    曾祥文

    2011-01-01

    随着图书馆数字资源总量的快速增加,传统的存储方式在面对海量数据存储的需求上,存在着容量和性能的瓶颈。云存储作为一种新型的存储服务模式,为图书馆的海量数字资源建设提供了一种新的选择。在分析图书馆数字资源建设需求、介绍云存储概念的基础上,对基于云存储模式下图书馆数字资源的建设进行了探讨。%With the rapid increase in quantity of the library digital resources,there is capacity and performance of bottleneck when the traditional way of storage in the face of mass storage requirements.As a new storage service mode,cloud storage provides a new choice for the amount of library digital resources construction.Based on the analysis of library digital resources construction demand and the introduction of the concept of cloud storage,this paper discussed the library digital resources construction based on the cloud storage mode.

  9. Study on Information Resource Services in Libraries under Network Environment%网络环境下图书馆信息资源服务研究

    Institute of Scientific and Technical Information of China (English)

    周亮; 袁艳

    2016-01-01

    The development of Internet era facilitates the development of information resource services in libraries and meanwhile brings new challenges. Network environment gives time features to information services in libraries, such as individualization of customer needs, simplicity of operation systems, diversification of information services, and mobilization of user behaviors. Based on current information resource service mode, problems of information resource services in libraries were examined, and development strategies were proposed to promote information resource services in libraries, and then better services for the public were provided.%互联网时代的发展促进了图书馆信息资源服务的发展,同时也为其带来了新挑战,网络环境赋予图书馆信息资源服务的时代特征,如用户需求个性化、系统操作简洁化、信息服务多样化、用户行为移动化等。以当前图书馆信息资源服务模式为基点,考察网络环境下图书馆信息资源服务面临的困境,提出发展策略,促进图书馆信息资源服务,更好地为广大读者服务。

  10. The Data Model Resource Book A Library of Universal Data Models for All Enterprises

    CERN Document Server

    Silverston, Len

    2008-01-01

    A quick and reliable way to build proven databases for core business functionsIndustry experts raved about The Data Model Resource Book when it was first published in March 1997 because it provided a simple, cost-effective way to design databases for core business functions. Len Silverston has now revised and updated the hugely successful First Edition, while adding a companion volume to take care of more specific requirements of different businesses. Each volume is accompanied by a CD-ROM, which is sold separately. Each CD-ROM provides powerful design templates discussed in the books in a rea

  11. Better Collaborative Working is Likely to Increase Uptake of Library Resources in a University Setting. A review of: Hightower, Barbara, Carolyn Rawl, and Michelle Schutt. “Collaborations for Delivering the Library to Students Through WebCT.” Reference Services Review 35.4 (2007: 541-51.

    Directory of Open Access Journals (Sweden)

    John Loy

    2008-06-01

    Full Text Available Objectives – To ascertain the extent to which university faculty members are integrating library resources within the WebCT course management system / managed learning environment. Also, to identify the reasons why faculty members are not integrating library resources within WebCT, and to explore their willingness to do so in future. Design – Case study with survey questionnaire and selected interview follow up.Setting – Urban campus of Auburn University in Montgomery, Alabama, United States of America.Subjects – One hundred and two members of faculty received a questionnaire. Five follow-up interviews were conducted.Methods – One hundred and two members of faculty with WebCT-supported courses received a questionnaire. They were asked to indicate if they linked to library resources, and if so which specific ones (e.g. library homepage, online catalogue, etc.. If they did not link to library resources they were asked to indicate the reason why not, either by selecting one or more of five pre-selected reasons, or by detailing their own. Follow-up interviews were conducted with five respondents, two of whom (Education and Nursing linked to library resources, and three of whom (from Business, Education and Science did not. Server log reports were also examined to identify how many students were entering library resources from WebCT.Main Results – The survey response rate was 28% (29 individuals, and of these Nursing and Sciences were the highest users with 7 individuals in each faculty. Of the 29 respondents only 7 (24% currently linked to library resources. Nursing were the biggest group with 10 links (38% of the total, Science with 6 (23% and 5 each from Liberal Arts and Education (19%. The resources chosen to link to were• Article databases (6• Library homepage (5• Ask a Librarian e-mail service(3• Net library e-book collection (3Six other library resources scored lower and two were not linked to at all. While few respondents

  12. Engineering Faculty Indicate High Levels of Awareness and Use of the Library but Tend to Consult Google and Google Scholar First for Research Resources

    Directory of Open Access Journals (Sweden)

    Elaine Sullo

    2016-09-01

    Full Text Available A Review of: Zhang, L. (2015. Use of library services by engineering faculty at Mississippi State University, a large land grant institution. Science & Technology Libraries, 34(3, 272-286. http://dx.doi.org/10.1080/0194262X.2015.1090941 Objective – To investigate the engineering faculty’s information-seeking behaviour, experiences, awareness, and use of the university library. Design – Web-based survey questionnaire. Setting – The main campus of a state university in the United States of America. Subjects – 119 faculty members within 8 engineering departments. Methods – An email invitation to participate in a 16-item electronic survey questionnaire, with questions related to library use, was sent in the spring of 2015 to 119 engineering faculty members. Faculty were given 24 days to complete the survey, and a reminder email was sent 10 days after the original survey invitation. Main Results – Thirty-eight faculty members responded to the survey, representing a response rate of 32%. Overall, faculty had a high level of use and awareness of both online and physical library resources and services, although their awareness of certain scholarly communication services, such as data archiving and copyright advisory, was significantly lower. Faculty tend to turn to Google and Google Scholar when searching for information rather than turning to library databases. Faculty do not use social media to keep up with library news and updates. The library website, as well as liaison librarians, were cited as the primary sources for this type of information. Conclusions – The researcher concludes that librarians need to do a better job of marketing library resources, such as discipline-specific databases, as well as other library search tools. Because faculty use web search engines as a significant source of information, the author proposes further research on this behaviour, and suggests more action to educate faculty on different search tools

  13. HUMAN RESOURCE DEVELOPMENT IN ACADEMIC LIBRARIES IN THE AGE OF INFORMATION TECHNOLOGY AND NEED OF TRAINING : AN OVERVIEW.

    OpenAIRE

    KANADE MANJUSHA LAXMANRAO; SHYAMKANT J.DESHMUKH

    2013-01-01

    Development of any Nation is depends upon its quality of education. In this digital era libraries must adopt digital technologies. Academic library plays vital role in imparting education to its clients. Due to the rapid growth in the information generation and in varied forms, ever increasing information demands from users, now days libraries must convert their collection and services from traditional to the automated. With the help of computer and software, Libraries can provide right infor...

  14. 提高大学图书馆数字资源利用率的新举措%New Measures to Improve the Digital Resource Utilization of University Libraries

    Institute of Scientific and Technical Information of China (English)

    沈玉惠

    2013-01-01

    As an important part of university library information resources construction, digital resource has become the consensus of university libraries. This paper analyzes the main reasons of the low utilization rate of digital resources in university libraries, proposes new measures from several aspects to improve the resource utilization of university libraries, to provide reference for the construction of digital library resources.%数字资源作为大学图书馆信息资源建设的重要内容,已成为各大学图书馆的共识。文章分析大学图书馆数字资源利用率偏低的主要原因,指出从多个方面提高大学图书馆数字资源利用率,以期为图书馆数字资源建设提供参考。

  15. Construction and Identification of Bacterial Artificial Chromosome Library for 0-613-2R in Upland Cotton

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    A bacterial artificial chromosome (BAC) library containing a large genomic DNA insert is an important tool for genome physical mapping, map-based cloning, and genome sequencing. To isolate genes via a map-based cloning strategy and to perform physical mapping of the cotton genome, a high-quality BAC library containing large cotton DNA inserts is needed. We have developed a BAC library of the restoring line 0-613-2R for isolating the fertility restorer (Rf1) gene and genomic research in cotton (Gossypium hirsutum L.). The BAC library contains 97 825 clones stored in 255 pieces of a 384-well microtiter plate. Random samples of BACs digested with the Notl enzyme indicated that the average insert size is approximately 130 kb, with a range of 80-275 kb,and 95.7% of the BAC clones in the library have an average insert size larger than 100 kb. Based on a cotton genome size of 2 250 Mb, library coverage is 5.7 x haploid genome equivalents. Four clones were selected randomly from the library to determine the stability of the BAC clones. There were no different fingerprints for 0 and 100 generations of each clone digested with Notl and Hindlll enzymes. Thus, the stability of a single BAC clone can be sustained at least for 100 generations. Eight simple sequence repeat (SSR) markers flanking the Rf1 gene were chosen to screen the BAC library by pool using PCR method and 25 positive clones were identified with 3.1 positive clones per SSR marker.

  16. 高校图书馆人力资源开发与管理探究%Study on Human Resources Development and Management in Academic Library --For library of Yangtze Normal University an e

    Institute of Scientific and Technical Information of China (English)

    刘晓莉

    2011-01-01

    Based on the state of human resources of Academic library, this article points out the problems of human resources management in libraries at present. Some suggestions, which includes librarian's inspection and training and the idea of human oriented, sho%文章就高校图书馆人力资源的现状进行分析,指出了存在的问题,提出了加强馆员的考核及培训和强化以人为本的理念是当前改善高校图书馆人力资源管理现状的重要举措。

  17. Prediction of Digital Resource Cost in the University Library%高校图书馆数字资源的成本预测

    Institute of Scientific and Technical Information of China (English)

    谭凯波

    2013-01-01

    The digital resource cost in the university library including purchase cost and use cost, and the cost prediction can provide a reference for the construction of digital resources in a university library. The Markov model and grey prediction model are combined to discuss a scientific prediction method about digital resource cost in the university.%高校图书馆数字资源成本主要包括购置成本与使用成本,将马尔可夫模型与灰色预测模型相结合,对成本进行科学预测,可以为高校图书馆数字资源建设提供决策参考。

  18. Invest Sharing the Library Information and Resource of Hebei United University%河北联合大学图书馆信息资源共享

    Institute of Scientific and Technical Information of China (English)

    王淑敏

    2014-01-01

    In this paper,based on the network environment to focus on the implementation of the construction of lit-erature information resources of Hebei United University,analyzed the practicability in the corse of library informa-tion resources construction,and put forward some countermeasures to strengthen the sharing construction of literature resources of Hebei United University library.%立足网络环境下实现河北联合大学文献信息资源建设,通过对图书馆信息资源建设存在的可行性分析,提出了加强河北联合大学图书馆文献资源共享建设的对策。

  19. Remobilizing deleted piggyBac vector post-integration for transgene stability in silkworm.

    Science.gov (United States)

    Wang, Feng; Wang, Riyuan; Wang, Yuancheng; Xu, Hanfu; Yuan, Lin; Ding, Huan; Ma, Sanyuan; Zhou, You; Zhao, Ping; Xia, Qingyou

    2015-06-01

    Deletion of transposable elements post-genomic integration holds great promise for stability of the transgene in the host genome and has an essential role for the practical application of transgenic animals. In this study, a modified piggyBac vector that mediated deletion of the transposon sequence post-integration for transgene stability in the economically important silkworm Bombyx mori was constructed. The piggyBac vector architecture contains inversed terminal repeat sequences L1, L2 and R1, which can form L1/R1 and L2/R1 types of transposition cassettes. hsp70-PIG as the piggyBac transposase expression cassette for initial transposition, further remobilization and transgene stabilization test was transiently expressed in a helper vector or integrated into the modified vector to produce a transgenic silkworm. Shortening L2 increased the transformation frequency of L1/R1 into the silkworm genome compared to L2/R1. After the integration of L1/R1 into the genome, the remobilization of L2/R1 impaired the transposon structure and the resulting transgene linked with an impaired transposon was stable in the genome even in the presence of exogenously introduced transposase, whereas those flanked by the intact transposon were highly mobile in the genome. Our results demonstrated the feasibility of post-integration deletion of transposable elements to guarantee true transgene stabilization in silkworm. We suggest that the modified vector will be a useful resource for studies of transgenic silkworms and other piggyBac-transformed organisms. PMID:25589404

  20. Resource Construction of College Library under the Background of Big Data%大数据背景下高校图书馆的资源建设

    Institute of Scientific and Technical Information of China (English)

    王晔煊; 杨艳君

    2013-01-01

    大数据时代,图书馆将在资源建设、读者服务、组织管理等方面面临着严峻的挑战。数字图书馆是图书馆信息化建设的重要组成部分。文章论述了大数据的内涵,分析了高校数字资源建设中存在的问题,进而对图书馆数字资源建设进行探讨。%Libraries will face severe challenges in terms of resource construction,reader service and organiza-tional management in an era of big data. Digital library is an important part of informatization of library. The paper discusses the connotation of big data,analyzes the problems facing digital resource construction of colle-ges and further explores the digital resource construction of library under background of big data.

  1. 长清大学城图书馆文献信息资源共享探究%Changqing University City Library to Explore the Sharing of Information Resources

    Institute of Scientific and Technical Information of China (English)

    王少华; 王海燕

    2011-01-01

    University City Library resource sharing is the important areas of resource sharing, document sharing is the core of library resource sharing, Changqing University City Library literature resource sharing is the only way for the development of universitie%图书馆资源共享是大学城资源共享的重要领域之一,文献信息资源共享则是图书馆资源共享的核心。长清大学城图书馆文献信息资源共享也是各高校发展的必由之路。

  2. Sports Education Library Information Services

    Institute of Scientific and Technical Information of China (English)

    许晓峰

    2014-01-01

    Library website, is the first window for readers to understand library information services. Sports Education academy library fully take advantage of homepage, combine open access resource searched with the library collections, after targeted collection, selection, sorting, processing, clustering or reorganization, establish a navigation system of open access resource of physical Sports Education.

  3. Transposon-mediated BAC transgenesis in human ES cells.

    Science.gov (United States)

    Rostovskaya, Maria; Fu, Jun; Obst, Mandy; Baer, Isabell; Weidlich, Stefanie; Wang, Hailong; Smith, Andrew J H; Anastassiadis, Konstantinos; Stewart, A Francis

    2012-10-01

    Transgenesis is a cornerstone of molecular biology. The ability to integrate a specifically engineered piece of DNA into the genome of a living system is fundamental to our efforts to understand life and exploit its implications for medicine, nanotechnology and bioprospecting. However, transgenesis has been hampered by position effects and multi-copy integration problems, which are mainly due to the use of small, plasmid-based transgenes. Large transgenes based on native genomic regions cloned into bacterial artificial chromosomes (BACs) circumvent these problems but are prone to fragmentation. Herein, we report that contrary to widely held notions, large BAC-sized constructs do not prohibit transposition. We also report the first reliable method for BAC transgenesis in human embryonic stem cells (hESCs). The PiggyBac or Sleeping Beauty transposon inverted repeats were integrated into BAC vectors by recombineering, followed by co-lipofection with the corresponding transposase in hESCs to generate robust fluorescent protein reporter lines for OCT4, NANOG, GATA4 and PAX6. BAC transposition delivers several advantages, including increased frequencies of single-copy, full-length integration, which will be useful in all transgenic systems but especially in difficult venues like hESCs. PMID:22753106

  4. A first generation BAC-based physical map of the rainbow trout genome

    Directory of Open Access Journals (Sweden)

    Thorgaard Gary H

    2009-10-01

    Full Text Available Abstract Background Rainbow trout (Oncorhynchus mykiss are the most-widely cultivated cold freshwater fish in the world and an important model species for many research areas. Coupling great interest in this species as a research model with the need for genetic improvement of aquaculture production efficiency traits justifies the continued development of genomics research resources. Many quantitative trait loci (QTL have been identified for production and life-history traits in rainbow trout. A bacterial artificial chromosome (BAC physical map is needed to facilitate fine mapping of QTL and the selection of positional candidate genes for incorporation in marker-assisted selection (MAS for improving rainbow trout aquaculture production. This resource will also facilitate efforts to obtain and assemble a whole-genome reference sequence for this species. Results The physical map was constructed from DNA fingerprinting of 192,096 BAC clones using the 4-color high-information content fingerprinting (HICF method. The clones were assembled into physical map contigs using the finger-printing contig (FPC program. The map is composed of 4,173 contigs and 9,379 singletons. The total number of unique fingerprinting fragments (consensus bands in contigs is 1,185,157, which corresponds to an estimated physical length of 2.0 Gb. The map assembly was validated by 1 comparison with probe hybridization results and agarose gel fingerprinting contigs; and 2 anchoring large contigs to the microsatellite-based genetic linkage map. Conclusion The production and validation of the first BAC physical map of the rainbow trout genome is described in this paper. We are currently integrating this map with the NCCCWA genetic map using more than 200 microsatellites isolated from BAC end sequences and by identifying BACs that harbor more than 300 previously mapped markers. The availability of an integrated physical and genetic map will enable detailed comparative genome

  5. Study in Grey and White: Measuring the Impact of the 8Rs Canadian Library Human Resources Study

    Directory of Open Access Journals (Sweden)

    Allison Sivak

    2009-06-01

    Full Text Available Objective – To use the 8Rs Canadian Library Human Resources Study (the 8Rs Study as a test case to develop a model for assessing research impact in LIS. Methods – Three different methods of citation analysis which take into account the changing environment of scholarly communications. These include a ‚manual‛ method of locating citations to the 8Rs Study through a major LIS database, an enhanced-citation tool Google Scholar, and a general Google search to locate Study references in non-scholarly documents Results – The majority of references (82% were found using Google or Google Scholar; the remainder were located via LISA. Each method had strengths and limitations.Conclusion - In-depth citation analysis provides a promising method of understanding the reach of published research. This investigation’s findings suggest the need for improvements in LIS citation tools, as well as digital archiving practices to improve the accessibility of references for measuring research impact. The findings also suggest the merit of researchers and practitioners defining levels of research impact, which will assist researchers in the dissemination of their work.

  6. Genealogy Resources Construction in Guangzhou Library%广州图书馆的家谱资源建设

    Institute of Scientific and Technical Information of China (English)

    何映雯

    2014-01-01

    ABSTRACT:The genealogy, official history and chorography constitute the three pillars of Chinese historical documents, they supplement historical facts from different angles, making the historical appearance, time spirit and social custom get more real and more comprehensive presentation, and have an irreplaceable special function for the deep study of history, demography, sociology and other subjects. This paper expounds the significance of the construction of genealogy resources, and probes into the works that Guangzhou Library has done in aspects of genealogy resources’ collection, sorting, development, and service, etc.%家谱与正史、方志是构成中华历史文献的三大支柱,它们从不同角度补充史实,使历史面貌、时代精神和社会风尚得以更真实、更全面的呈现,对历史学、人口学及社会学等学科的深入研究具有不可替代的独特功能。阐述了建设家谱资源的意义,探讨了广州图书馆在家谱资源的征集、整理、开发及服务方面所做的工作。

  7. Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species

    Directory of Open Access Journals (Sweden)

    Doležel Jaroslav

    2010-02-01

    Full Text Available Abstract Background The presence of closely related genomes in polyploid species makes the assembly of total genomic sequence from shotgun sequence reads produced by the current sequencing platforms exceedingly difficult, if not impossible. Genomes of polyploid species could be sequenced following the ordered-clone sequencing approach employing contigs of bacterial artificial chromosome (BAC clones and BAC-based physical maps. Although BAC contigs can currently be constructed for virtually any diploid organism with the SNaPshot high-information-content-fingerprinting (HICF technology, it is currently unknown if this is also true for polyploid species. It is possible that BAC clones from orthologous regions of homoeologous chromosomes would share numerous restriction fragments and be therefore included into common contigs. Because of this and other concerns, physical mapping utilizing the SNaPshot HICF of BAC libraries of polyploid species has not been pursued and the possibility of doing so has not been assessed. The sole exception has been in common wheat, an allohexaploid in which it is possible to construct single-chromosome or single-chromosome-arm BAC libraries from DNA of flow-sorted chromosomes and bypass the obstacles created by polyploidy. Results The potential of the SNaPshot HICF technology for physical mapping of polyploid plants utilizing global BAC libraries was evaluated by assembling contigs of fingerprinted clones in an in silico merged BAC library composed of single-chromosome libraries of two wheat homoeologous chromosome arms, 3AS and 3DS, and complete chromosome 3B. Because the chromosome arm origin of each clone was known, it was possible to estimate the fidelity of contig assembly. On average 97.78% or more clones, depending on the library, were from a single chromosome arm. A large portion of the remaining clones was shown to be library contamination from other chromosomes, a feature that is unavoidable during the

  8. Research on the Value Creation of Libraries Based on Information Resources%图书馆基于信息资源的价值创造研究

    Institute of Scientific and Technical Information of China (English)

    赵珊珊

    2011-01-01

    As the knowledge and information provided institutions, libraries confronts with how to optimize the utilization of information resources. There is a dynamic conversion process between information resources and value creation in libraries. In the value creation process, libraries should formulate strategic plan from the standpoint of strategic management process, adjust organization structure and implement management transform according to the staged change of external environment, plan and integrate resources, change the service mode, and realize the value creation based on information resources, so as to form the sustainable service advantage.%图书馆作为知识信息提供机构,面临着如何优化信息资源利用的问题。图书馆的信息资源从产生到价值创造之间存在一个动态的转化过程。图书馆在价值创造过程中,需要从战略管理流程角度进行战略规划,根据外部环境的阶梯式变化要求适时地进行组织结构调整和管理变革、规划和整合资源、转变服务模式,实现基于信息资源的价值创造,从而形成可持续的服务优势。

  9. Chromosome region-specific libraries for human genome analysis. Final progress report, 1 March 1991--28 February 1994

    Energy Technology Data Exchange (ETDEWEB)

    Kao, F.T.

    1994-04-01

    The objectives of this grant proposal include (1) development of a chromosome microdissection and PCR-mediated microcloning technology, (2) application of this microtechnology to the construction of region-specific libraries for human genome analysis. During this grant period, the authors have successfully developed this microtechnology and have applied it to the construction of microdissection libraries for the following chromosome regions: a whole chromosome 21 (21E), 2 region-specific libraries for the long arm of chromosome 2, 2q35-q37 (2Q1) and 2q33-q35 (2Q2), and 4 region-specific libraries for the entire short arm of chromosome 2, 2p23-p25 (2P1), 2p21-p23 (2P2), 2p14-p16 (wP3) and 2p11-p13 (2P4). In addition, 20--40 unique sequence microclones have been isolated and characterized for genomic studies. These region-specific libraries and the single-copy microclones from the library have been used as valuable resources for (1) isolating microsatellite probes in linkage analysis to further refine the disease locus; (2) isolating corresponding clones with large inserts, e.g. YAC, BAC, P1, cosmid and phage, to facilitate construction of contigs for high resolution physical mapping; and (3) isolating region-specific cDNA clones for use as candidate genes. These libraries are being deposited in the American Type Culture Collection (ATCC) for general distribution.

  10. A collection of books from Bielany in the Jagiellonian Library. Security, maintenance and availability of resources. A notice on the progress of work, June 2011

    Directory of Open Access Journals (Sweden)

    Jacek Partyka

    2011-12-01

    Full Text Available In the autumn of 2009 the Jagiellonian Library became the depositary of a large, valuable and interesting collection of printed books from the library of the Camaldolese Monastery in Bielany (Krakow. The work on processing the collection began almost immediately after all the prints had been taken over by the Jagiellonian Library. Two groups of employees have been involved in the work, both from the Resource Processing Department and the Antique Books Department, in line with the natural division into modern and old prints. The Camaldolese collection from Bielany has been enclosed in the monastery and unavailable to most readers; now the very books which for many years have been the source of knowledge, assistance and cognitive tools to a narrow circle of monks may be accessed by all interested persons. In the next year, after basic processing of the collection, the books will create an extremely interesting research opportunity.

  11. Construction of random sheared fosmid library from Chinese cabbage and its use for Brassica rapa genome sequencing project

    Institute of Scientific and Technical Information of China (English)

    Tae-Ho Park; Beom-Seok Park; Jin-A Kim; Joon Ki Hong; Mina Jin; Young-Joo Seol; Jeong-Hwan Mun

    2011-01-01

    As a part of the Multinational Genome Sequencing Project of Brassica rapa, linkage group R9 and R3 were sequenced using a bacterial artificial chromosome (BAC) by BAC strategy. The current physical contigs are expected to cover approximately 90% euchromatins of both chromosomes. As the project progresses, BAC selection for sequence extension becomes more limited because BAC libraries are restriction enzyme-specific. To support the project, a random sheared fosmid library was constructed. The library consists of 97536 clones with average insert size of approximately 40 kb corresponding to seven genome equivalents, assuming a Chinese cabbage genome size of 550 Mb. The library was screened with primers designed at the end of sequences of nine points of scaffold gaps where BAC clones cannot be selected to extend the physical contigs. The selected positive clones were end-sequenced to check the overlap between the fosmid clones and the adjacent BAC clones.Nine fosmid clones were selected and fully sequenced. The sequences revealed two completed gap filling and seven sequence extensions, which can be used for further selection of BAC clones confirming that the fosmid library will facilitate the sequence completion of B. rapa.

  12. Vocational Colleges Steam Inspection Professional Teaching Resource Library Construction%高职院校汽检专业教学资源库建设

    Institute of Scientific and Technical Information of China (English)

    马玲

    2013-01-01

      文章结合高职教育人才培养方案,提出了专业教学资源库建设的思路和内容,专业教学资源库主要内容包括专业教学标准、课程资源、数字化教学资源、职业能力拓展资源和网上交流等五个方面建设。%In this paper, combined with higher vocational education personnel training plan, put forward professional teaching resources repository construction train of thought and content, teaching resource library includes professional teaching standards, curriculum resource, digital teaching resources, occupation ability development resources and the Internet in five aspects of construction.

  13. L’italiano alla prova del Bac

    Directory of Open Access Journals (Sweden)

    Giulia Massignan

    2013-07-01

    Full Text Available Il lavoro propone alcune attività didattiche di preparazione dell’esame orale di italiano previsto dal Baccalauréat del liceo francese. Tale prova d’esame è stata recentemente oggetto di una riforma ministeriale volta a dare maggior peso alla pratica orale. Dopo uno studio delle nuove modalità della prove d’esame e dei suoi contenuti, ci si concentrerà sull’analisi delle abilità orali necessarie al suo superamento e sugli elementi linguistici e comunicativi su cui porre l’attenzione. Sulla base di queste premesse teoriche e metodologiche, si presentano alcuni interventi didattici di preparazione all’esame: due attività per la prova di comprensione orale e due per la prova di produzione e interazione orale; in entrambi i casi, si insiste sull’importanza di proporre strategie d’ascolto e di parlato insegnabili, che diventino consuetudini attive e parte integrante del bagaglio comunicativo del discente. The italian exam in the FrenchBacThe paper proposes some educational activities to prepare for the oral Italian examination as part of the French Baccalauréat. This exam has recently been the subject of a ministerial reform aimed at giving more weight to the oral practice. After illustrating new examination methods and content, we will focus on the analysis of oral skills necessary for passing the exam and aspects of language and communication on which to focus. Based on these theoretical and methodological premises, some educational exam preparation activities will be presented. Two activities test listening comprehension and two focus on production and oral interaction. The stress is on the importance of proposing teach able listening and speaking strategies, so that they become active habits and integral parts of the communicative learner’s baggage.

  14. The Egyptian Web Logs as an Information Resource with a Special Indication to Library and Librarian's Blogs

    Directory of Open Access Journals (Sweden)

    Shayma' Ismael Abbas Ismael

    2007-06-01

    Full Text Available An article is discussing The Egyptian Web Logs ,library blogs and librarian's blogs at the world wide web as one of information electronic sources so this article is answering on some questions. what is conception of blogs?, What is historic development and origin of blogs?, What are reasons to fame and spread blogs between internet employees ?, what is blogging software to create blog?, what is directory and search engine of blogs?, what is characteristics of successful blogs? and concentrates on Egyptian weblogs, what is the most famous Egyptian blogs in the world wide web ? and refer to relationship between the Egyptian weblogs and policy. and special indication to blogs in library and information science which include library blogs and librarian's blogs. this article also is answering on some questions about library blogs and librarian's blogs. what is meaning of library blogs and librarian's blogs? what is purposes and aims of library blogs and librarian's blogs? what is directory of library blogs and librarian's blogs? Are there sorts of library blogs and librarian's blogs? and What are examples of library blogs and librarian's blogs available at the world wide?. to give overview about this electronic source at all its parts.

  15. Discrimination and Analysis on Co-construction and Sharing of Resources between College Libraries and Public Libraries%高校图书馆与公共图书馆资源共建共享问题辨析

    Institute of Scientific and Technical Information of China (English)

    宋丽花

    2012-01-01

    介绍了高校图书馆与公共图书馆资源共享实现的可行性,得出信息时代环境中,要推进高校图书馆与公共图书馆资源的共建共享,应当建立健全组织机构,打破条块分割的行政体制障碍;合作建设馆藏,统一规划收集信息资源;完善标准化建设,建立文献资源共建共享信息平台。%The author introduced feasibility of realizing co-construction and sharing of resources between college libraries and public libraries.In order to promote co-construction and sharing of resources,the author put forward specific measures: establishing and improving organization structure,breaking administrative system barrier,building archives storage,integrated planning collection information resources,perfecting standardization construction and building co-construction and sharing platform of literature resources.

  16. There is a Relationship between Resource Expenditures and Reference Transactions in Academic Libraries. A Review of: Dubnjakovic, A. (2012. Electronic resource expenditure and the decline in reference transaction statistics in academic libraries. Journal of Academic Librarianship, 38(2, 94-100. doi:10.1016/j.acalib.2012.01.001

    Directory of Open Access Journals (Sweden)

    Annie M. Hughes

    2013-03-01

    Full Text Available Objective – To provide an analysis of the impact of expenditures on electronic resourcesand gate counts on the increase or decrease in reference transactions.Design – Analysis of results of existing survey data from the National Center for Educational Statistics (NCES 2006 Academic Library Survey(ALS.Setting – Academic libraries in the United States.Subjects – 3925 academic library respondents.Methods – The author chose to use survey data collected from the 2006 ALS conducted bythe NCES. The survey included data on various topics related to academic libraries, but in the case of this study, the author chose to analyze three of the 193 variables included. The three variables: electronic books expenditure, computer hardware and software, and expenditures on bibliographic utilities, were combined into one variable called electronic resource expenditure. Gate counts were also considered as a variable. Electronic resource expenditure was also split as a variable into three groups: low, medium, and high. Multiple regression analysis and general linear modeling, along with tests of reliability, were employed. Main Results – The author determined that low, medium, and high spenders with regard to electronic resources exhibited differences in gate counts, and gate counts have an effect on reference transactions in any given week. Gate counts tend to not have much of an effect on reference transactions for the higher spenders, and higher spenders tend to have a higher number of reference transactions overall. Low spenders have lower gate counts and also a lower amount of reference transactions.Conclusion – The findings from this study show that academic libraries spending more on electronic resources also tend to have an increase with regard to reference transactions. The author also concludes that library spaces are no longer the determining factor with regard to number of reference transactions. Spending more on electronic resources is

  17. 图书馆的特色资源建设研究%Research on the Construction of Library Characteristic Resources

    Institute of Scientific and Technical Information of China (English)

    陈玉梅

    2014-01-01

    图书馆的特色资源建设在近些年来成为热点词汇,面对当前我国图书馆特色资源建设面临的诸多弊病,本文通过成功的范例阐述了通过PDA、联邦式共享等方式对图书馆的特色资源建设的改革思路。%The construction of library characteristic resources has become a hot topic in recent years. Faced with the shortcomings of the current construction of library characteristic resources in China, this paper, baseds on successful examples, elaborated the reform ideas through the method of PDA and cooperative sharing.

  18. On the Effective Development of Library Resources from the Perspective of Supply and Demand%供求视野下文献资源的有效开发研究

    Institute of Scientific and Technical Information of China (English)

    王鑫

    2012-01-01

    Based on an analysis of the nature of library resources in universities from the perspective of supply and demand,this paper points out that library resources,as a kind of shared resources with limited access,sometimes suffer from conflicts between supply and demand.Therefore,it is necessary to optimize the management of library resources and enhance studies on how to effectively develop library resources.%从供求视野角度分析了高校图书馆文献信息资源的性质,指出文献资源作为一种稀缺性的共有资源,它有时存在供给和需求矛盾,需要人们对图书馆文献资源的管理进行优化管理,强化文献资源的有效开发研究。

  19. Research on the Construction of Music Resources in University Library%关于普通高校图书馆音乐资源建设的研究

    Institute of Scientific and Technical Information of China (English)

    戴蕾

    2014-01-01

    阐述了普通高校图书馆音乐资源建设的意义,分析了普通高校图书馆音乐资源的现状,提出了普通高校图书馆音乐资源建设的策略。%This paper briefly expounds the significance of the construction of music resources in regular university library,analyzes the current status of music resources in regular university library,and puts forward some strategies for the construction of music resources in regular university library.

  20. Discussion on Information Resources Construction of University Library in Network Environment%网络环境下高校图书馆信息资源建设探析

    Institute of Scientific and Technical Information of China (English)

    董昌

    2015-01-01

    分析了网络环境下影响高校图书馆信息资源建设的因素,介绍了高校图书馆信息资源建设的内容,探讨了网络环境下拓展高校图书馆信息资源建设的若干要点.%This paper describes the factors influencing university library's information resource construction in network environment,introduces the contents of university library's information resource construction,and probes into some points of expanding university library's information resource construction in network environment.

  1. 论图书馆数字馆藏资源建设中亟待解决的问题%Discussion of Problems in the Construction of the Digital Resources in Libraries

    Institute of Scientific and Technical Information of China (English)

    张德云

    2012-01-01

    In the article the generation, concept and characteristics of the digital resources in libraries were firstly discussed, and the problems in the construction of the digital resources in libraries were analyzed. On the basis of this analysis, the strategies for the construction of the digital resources in libraries were explored.%论述数字馆藏的产生、概念和特点,分析数字馆藏资源建设中存在的问题,探讨数字馆藏资源建设中应采取的策略。

  2. The Library Lobby

    Science.gov (United States)

    Ralston, Anthony

    1971-01-01

    The library is examined as a university-wide resource and as a research facility. The use of the library relative to computing facilities is also considered as is the cost and value of library usage in relation to computer usage. (Author)

  3. Changing State Digital Libraries

    Science.gov (United States)

    Pappas, Marjorie L.

    2006-01-01

    Research has shown that state virtual or digital libraries are evolving into websites that are loaded with free resources, subscription databases, and instructional tools. In this article, the author explores these evolving libraries based on the following questions: (1) How user-friendly are the state digital libraries?; (2) How do state digital…

  4. Are You Overlooking a Valuable Resource? A Practical Guide to Collaborating with Your Greatest Ally: The Public Library

    Science.gov (United States)

    DelGuidice, Margaux

    2009-01-01

    The author's first "real job" when she was fresh out of library school was working as a leave replacement for an elementary school media specialist. One of her first tasks as the interim media specialist was to help organize the fourth grade science fair. Using her recently acquired library school skills, she decided to call the local public…

  5. Web 2.0 as Catalyst: Virtually Reaching Out to Users and Connecting Them to Library Resources and Services

    Science.gov (United States)

    Xiao, Norah

    2008-01-01

    This article is based on services to library users in the area of chemistry at the Science and Engineering (S&E) Library of the University of Southern California (USC), to which I applied various new technologies as outreach approaches. Various Web 2.0 technologies such as a blog, tags, YouTube, RSS feeds, Instant Messaging, online presentation…

  6. Gains and Losses of Colleges and Universities Libraries Resources Sharing in Shanxi Province%山西高校图书馆资源共享之得失

    Institute of Scientific and Technical Information of China (English)

    赵冬梅

    2012-01-01

    In the state of nationwide colleges and universities libraries resource sharing, after several years of efforts, colleges and universities libraries resource sharing in Shanxi province has made some achievements accompanying with many obstacles. By reviewing the gains and losses, the aim of the article is to avoid the resistance and to make clear the direction for the future development of colleges and universities libraries resources sharing in Shanxi prov- ince.%基于全国高校图书馆资源共享的大环境,山西省经过几年的努力在资源共享方面也取得了一定的成绩,但也存在诸多的阻力,本文通过回顾山西高校图书馆资源共享之得失,旨在避免未来发展的阻力,明确未来发展的方向。

  7. On the Cloud Storage Mode of University Library Digital Resources%高校图书馆数字资源云存储模式探究

    Institute of Scientific and Technical Information of China (English)

    施蓉蓉

    2012-01-01

    With the rapid increase in quantity of library digital resources,the drawbacks of traditional data storage model increasingly appear.As a new storage service mode,cloud storage provides a new choice for the amount of li-brary digital resources construction.This paper introduces some digital storage technologies,and discusses the application of cloud storage in university library digital resources storage.%随着图书馆数字资源总量的快速增加,传统的数据存储方式弊端日益显现。云存储作为一种新型的存储服务模式,为图书馆的海量数字资源建设提供了一种新的选择。在介绍数字存储技术的基础上,重点探讨了云存储在高校图书馆数字资源存储中的应用。

  8. Research on construction of Digital Resources in University Library%高职院校图书馆数字信息资源建设研究

    Institute of Scientific and Technical Information of China (English)

    马丽华

    2011-01-01

    数字图书馆时代,高校图书馆数字信息资源建设势在必行。本文分析了北京地区示范性高职院校图书馆数字信息资源建设的现状、问题及对策。%In the age of Digital library,Digital resources construction is imperative.The paper analyzed the current status,issues,strategies and solutions on digital resources construction in the vocational colleges of Beijing.

  9. Canadian Library Human Resources Short‐Term Supply and Demand Crisis Is Averted, But a Significant Long‐Term Crisis Must Be Addressed. A review of: 8Rs Research Team. The Future of Human Resources in Canadian Libraries February 2005. Edmonton, AB: University of Alberta. 21 February 2007 .

    OpenAIRE

    Julie McKenna

    2007-01-01

    Objective – To examine the human resources environment in Canadian libraries in order to assess readiness to accommodate change and to identify opportunities for human resources planning. The “8Rs” of the study were defined as recruitment, retirement, retention, remuneration, repatriation, rejuvenation, re‐accreditation, and restructuring.Design – This study was undertaken in three phases over nearly three years through the use a variety of methods including literature review, analyses of exi...

  10. Completely Distinguishing Individual A-genome Chromosomes and Their Karyotyping Analysis by Multiple BAC-FISH

    Institute of Scientific and Technical Information of China (English)

    WANG Kai; GUO Wang-zhen; ZHANG Tian-zhen

    2008-01-01

    @@ Multiple BAC-FISH is a powerful tool for modern cytogenetic researching in both animals and plants.But in cotton,this technique is unavailable due to the high percentage of repetitive sequences.Here,we identified twenty BACs from more than fifty BACs,and successfully demonstrated the use of multiple BAC-FISH for cytogenetie research in a diploid cotton species,G.arboreum.The karyotyping should be a basic application of this technique,but the potential usage such as high-resolution physical mapping construction,assisting BAC-by-BAC sequencing will be invaluable.

  11. On the Five Laws of Library Science of the guiding role of the school library literature resources construction%浅谈图书馆学五定律对学校图书馆文献资源建设的指导作用

    Institute of Scientific and Technical Information of China (English)

    申晓洁

    2012-01-01

      Five Laws of Library Science, and analysis of its role in guiding the Library Document Resources Construction. The information re-sources carrier should Five Laws of Library Science Literature Resource Construction of basic principles.%  介绍了图书馆学五定律的基本内容,分析了其对图书馆文献资源建设的指导作用。认为不论信息资源载体如何,都应以图书馆学五定律为文献资源建设的基本原则。

  12. The first generation of a BAC-based physical map of Brassica rapa

    Directory of Open Access Journals (Sweden)

    Lee Soo

    2008-06-01

    Full Text Available Abstract Background The genus Brassica includes the most extensively cultivated vegetable crops worldwide. Investigation of the Brassica genome presents excellent challenges to study plant genome evolution and divergence of gene function associated with polyploidy and genome hybridization. A physical map of the B. rapa genome is a fundamental tool for analysis of Brassica "A" genome structure. Integration of a physical map with an existing genetic map by linking genetic markers and BAC clones in the sequencing pipeline provides a crucial resource for the ongoing genome sequencing effort and assembly of whole genome sequences. Results A genome-wide physical map of the B. rapa genome was constructed by the capillary electrophoresis-based fingerprinting of 67,468 Bacterial Artificial Chromosome (BAC clones using the five restriction enzyme SNaPshot technique. The clones were assembled into contigs by means of FPC v8.5.3. After contig validation and manual editing, the resulting contig assembly consists of 1,428 contigs and is estimated to span 717 Mb in physical length. This map provides 242 anchored contigs on 10 linkage groups to be served as seed points from which to continue bidirectional chromosome extension for genome sequencing. Conclusion The map reported here is the first physical map for Brassica "A" genome based on the High Information Content Fingerprinting (HICF technique. This physical map will serve as a fundamental genomic resource for accelerating genome sequencing, assembly of BAC sequences, and comparative genomics between Brassica genomes. The current build of the B. rapa physical map is available at the B. rapa Genome Project website for the user community.

  13. Library news

    CERN Multimedia

    CERN Library

    2010-01-01

    The CERN Library has been providing electronic access to the "Techniques de l'Ingénieur" database for the past 8 months. As a reminder, this is a multidisciplinary database of over 4000 technical and scientific articles in French, covering a broad range of topics such as mechanical engineering, safety, electronics and the environment. In a few simple steps, you can create your own account, select the types of documents you are interested in and configure your settings so as to receive alerts when articles in your field of activity are published. You can now access this resource from outside CERN using the "remote access to electronic resources" service. Further information is available here. Direct access to the database. Remote access to electronic resources. If you have any questions or comments, don't hesitate to contact us at: library.desk@cern.ch.

  14. The Physical Location of Gm-2 and Gm-6 in O.officinalis with BAC-FISH Based on Comparative RFLP Map of Wild Rice and Cultivated Rice

    Institute of Scientific and Technical Information of China (English)

    QIN Rui; WEI Wen-hui; NING Shun-bin; JIN Wei-wei; HE Guang-cun; SONG Yun-chun

    2002-01-01

    Rice BAC library is used widely in rice genome research due to its distinctive advantages over other library systems. In this study, two rice BAC clones closely linked to rice gall midge resistance, Gm-2 and Gm-6, were in situ hybridized to Oryza officinalis chromosomes. They were located on the long arm of chromosome 4 with FL 72.33% and 77.10% respectively and their FL was consistent with the selective marker of rice, RG214 and RZ569. The frequency of signal detection was 61.2% and 59.5% respectively.Our study was based on comparative RFLP map of wild rice, O. officinalis, and cultivated rice, O. sativa.

  15. 高校图书馆数字资源保存政策分析%Analysis of Digital Resource Preservation Policies of University Libraries

    Institute of Scientific and Technical Information of China (English)

    寇爽

    2012-01-01

    University libraries play an important role in the digital resource preservation,so they should formulate reasonable and perfect long-term preservation policies to guide the digital resource preservation.Digital resource preservation policies of university libraries in American could make into comprehensive policies and specialized policies,these policies have same points: making sure the mission or purpose of the digital resource preservation should be consistent with the mission of the university,describing the information of preservation objects clearly,giving prominence to the position of the non-technical factors including financial support and employee,and indicating that they should establish cooperation mechanisms.Digital resource preservation activities start late in China,and digital resource preservation policies of university libraries are relatively less.Therefore,according to actual situations of China and the successful experience of foreign countries,university libraries should formulate digital resource preservation policies from the preservation mission,preservation subjects,preservation objects,preservation strategies,safeguard mechanisms,and cooperation mechanisms.%高校图书馆在数字资源保存方面发挥着重要作用,应制定合理、完善的长期保存政策来引导数字资源保存的实践。美国高校图书馆数字资源保存政策分为综合性政策和专门性政策,这些政策都明确表示数字资源保存的使命(目的)应与大学本身的使命一致,并明确描述了保存客体(对象)的相关信息,突出了财政支持和员工等非技术因素的地位,明确指出要建立合作机制。我国数字资源保存活动起步较晚,高校图书馆的数字资源保存政策相对较少。我国高校图书馆应根据实际情况,借鉴国外的成功经验,从保存使命、保存主体、保存客体、保存策略、保障机制、合作机制等方面着手制定数字资源保存政策。

  16. Challenges and Opportunities for Libraries in Pakistan

    OpenAIRE

    Shafiq UR, Rehman; Pervaiz, Ahmad

    2007-01-01

    Abstract: This paper, based on review of literature, observation, and informal conversations, discusses various challenges regarding finance, collection development, ICTs, human resources, library education, library association and research & development faced by library profession in Pakistan. The opportunities to meet these challenges have also been explored. Keywords: Library challenges and opportunities (Pakistan); Librarianship (Pakistan); Library issues; Library profession in Pa...

  17. Digital Reading and the Information Resource Development of Libraries%数字阅读与图书馆信息资源建设

    Institute of Scientific and Technical Information of China (English)

    肖希明; 曾粤亮

    2016-01-01

    数字阅读时代的到来给图书馆信息资源建设带来了前所未有的机遇与挑战。本文从数字阅读的普及环境、发展态势以及知识获取地位等方面论述数字阅读的趋势与常态;剖析了数字阅读的特点及其在资源结构、资源建设模式、资源采集方式及资源购置经费结构等方面对图书馆信息资源建设带来的影响;认为在数字阅读背景下,图书馆应当贯彻“虚实并重”的资源建设基本方针,积极为数字阅读提供条件,参与数字资源生产并提供优质数字资源,开发网络资源并引导数字阅读,积极推进公共数字文化资源整合。%Digital reading has brought unprecedented opportunities and chal enges for the development of library information resources. The article discusses the normal and trends of digital reading in terms of its popularity, development situation and the status of obtaining knowledge. It analyses the characteristics of digital reading and its impact on libraries from the perspective of the resource structure, resource development modes, resource acquisition methods and expenditure structure of resource al ocation. In the background of digital reading, libraries should lay equal stress on both virtual and physical resources, create conditions for readers, participate in the production of digital resources and provide services, develop network resources and offer guidance and actively promote the integration of public digital cultural resources.

  18. Public librarians as a resource for promoting health: results from the Health for Everyone in Libraries Project (HELP) librarian survey.

    Science.gov (United States)

    Linnan, Laura A; Wildemuth, Barbara M; Gollop, Claudia; Hull, Peggy; Silbajoris, Christie; Monnig, Ruth

    2004-04-01

    Public libraries are located in all communities, and two thirds of adults visit one each year. Libraries give the public access to computers and the Internet, and librarians offer technical assistance for accessing information. The interests and training needs of public librarians for assisting the public in accessing health information have not been addressed. One public library/librarian in each North Carolina county was randomly selected to complete a written questionnaire to assess health-related information services and librarians' skills for providing these services. 84% of librarians (83/99) completed the questionnaire. Results indicate that librarians answer more than 10 health-related questions per week, feel moderately comfortable answering these questions, and are very interested in receiving additional training for addressing health-related questions. Creating public library/public health partnerships holds much promise for enhancing the ability of community members to access desired health information.

  19. 浅谈公共图书馆的人力资源开发与管理%On the Development and Management of Human Resources at Public Libraries

    Institute of Scientific and Technical Information of China (English)

    许迎霞

    2012-01-01

    提出了新世纪对图书馆员素质要求,图书馆应该在日常工作中开发人力资源,要加强人力资源管理,要优化人员结构,建立公平竞争机制,引入激励机制,强化考核手段,建立有效的培训机制,以图书馆文化感染人,制定合理、有效的人力资源开发与管理规则,以实现人力资源管理的科学化、规范化和民主化。%The paper according to the demand of quality of librarians in the new century, The author Proposed the library should develop the human resources in the routine work, Must strengthen the human resources management, must optimize the personnel structure, establishes the fair competition mechanism, the introduction drives the mecha- nism, the strengthened inspection method, establishes the effective training mechanism, by library culture infection person, formulation reasonable, effective human resources development and management rule, realizes the human resources management scientific style, the standardization and the democratization

  20. The Construction of Information Resources of University Library under Open Access Environment%开放存取环境下的高校图书馆信息资源建设

    Institute of Scientific and Technical Information of China (English)

    陈建华

    2012-01-01

    The paper analyses the impact of open access on the constructon of library information resources,puts forward the construction strategies of information resources of university library under open access environment.%通过分析开放存取对图书馆信息资源建设带来的影响,提出开放存取环境下高校图书馆信息资源建设策略。

  1. 谈地方高校图书馆数字资源建设%Discussion on the Construction of Digital Resources in Local University Library

    Institute of Scientific and Technical Information of China (English)

    焦玲霞

    2011-01-01

    通过对数字资源投资比重与不同载体资源的获取行为的分析可知,数字资源与纸质资源获取行为的趋势呈反向关系;数字资源投资比重越大,两载体资源的获取分化趋势就越明显。调研结果表明,高校图书馆应适当控制数字资源投资比重,降低对数字资源的依赖性;引进数字资源需量力而行;在增加数字资源投资比重的同时,应同步提高数字资源的服务质量。%Through the analysis on the investment ratio of digital resources and readers' acquirement actions of different media,we can find that the trend for acquiring digital resources and printed resources are opposite;as the investment ratio of digital resources becomes larger,the differentiation trends of digital resources and printed resources is more obvious.Results of survey study show that university libraries should control the investment ratio of digital resources moderately,in order to reduce the dependence on digital resources;acquire digital resources within libraries' capabilities;improve the service quality of digital resources when increasing the investment ratio of digital resources.

  2. An overview of the Phalaenopsis orchid genome through BAC end sequence analysis

    Directory of Open Access Journals (Sweden)

    Hsiao Yu-Yun

    2011-01-01

    Full Text Available Abstract Background Phalaenopsis orchids are popular floral crops, and development of new cultivars is economically important to floricultural industries worldwide. Analysis of orchid genes could facilitate orchid improvement. Bacterial artificial chromosome (BAC end sequences (BESs can provide the first glimpses into the sequence composition of a novel genome and can yield molecular markers for use in genetic mapping and breeding. Results We used two BAC libraries (constructed using the BamHI and HindIII restriction enzymes of Phalaenopsis equestris to generate pair-end sequences from 2,920 BAC clones (71.4% and 28.6% from the BamHI and HindIII libraries, respectively, at a success rate of 95.7%. A total of 5,535 BESs were generated, representing 4.5 Mb, or about 0.3% of the Phalaenopsis genome. The trimmed sequences ranged from 123 to 1,397 base pairs (bp in size, with an average edited read length of 821 bp. When these BESs were subjected to sequence homology searches, it was found that 641 (11.6% were predicted to represent protein-encoding regions, whereas 1,272 (23.0% contained repetitive DNA. Most of the repetitive DNA sequences were gypsy- and copia-like retrotransposons (41.9% and 12.8%, respectively, whereas only 10.8% were DNA transposons. Further, 950 potential simple sequence repeats (SSRs were discovered. Dinucleotides were the most abundant repeat motifs; AT/TA dimer repeats were the most frequent SSRs, representing 253 (26.6% of all identified SSRs. Microsynteny analysis revealed that more BESs mapped to the whole-genome sequences of poplar than to those of grape or Arabidopsis, and even fewer mapped to the rice genome. This work will facilitate analysis of the Phalaenopsis genome, and will help clarify similarities and differences in genome composition between orchids and other plant species. Conclusion Using BES analysis, we obtained an overview of the Phalaenopsis genome in terms of gene abundance, the presence of repetitive

  3. Printed Resources and Electronic Resources Integrated Services in Small and Medium-sized Libraries%中小型图书馆印本资源与电子资源一体化服务实践

    Institute of Scientific and Technical Information of China (English)

    刘胜

    2014-01-01

    Through analyzing the integrated service system of library resources, this paper sums up three methods for the integration of printed resources and digital resources including the digital resources catalog, digital resources navigate and open link system, and suggests that the libraries should give priority to books and periodicals integrated retrieval in the integrated service, and carry out the system construction within their capabilities by adopting the methods of making second development, introducing new system, and conducting independent development, etc.%通过对图书馆馆藏一体化服务系统的分析,归纳总结出印本资源与电子资源整合的3种主要模式———电子资源编目、电子资源导航与开放链接,并建议图书馆在进行一体化服务时,优先考虑图书和期刊的整合检索,在系统建设上量力而行,采取二次开发、引进新系统、自主开发等方式。

  4. Library Information Resource Integration System Study%图书馆信息资源整合系统的探究

    Institute of Scientific and Technical Information of China (English)

    郑剑

    2011-01-01

    随着数字图书馆建设进程的加快,各种类型、格式的数字资源急剧增加。但是因为数字资源的异构和分散、数据内容的交叉和重复、检索方式的多样性等等,致使用户面对浩瀚的信息海洋,往往无所适从,不知从何入手获取所需信息,他们不得不费很多时间学习各种数据库的使用方法、检索策略等。这样给用户造成很大的负担,并且严重影响了资源的利用率。为了解决这个问题,国内外图书馆都在积极开展数字资源整合工作,并已成为数字图书馆建设中一项迫切且艰巨的任务。鉴于资源整合工作对图书馆发展的重要性,数字资源整合研究已成为现阶段图书馆界研究的热门话题。在这样一个集群化的数字图书馆,具体的存贮介质与服务器的分布已经不重要,而重要的是数字图书馆所提供的服务与系统的功能。%With the acceleration of the process of building digital libraries,of all types and formats of digital resources has increased dramatically.But because the number of heterogeneous and distributed resources,data,content overlap and duplication,the diversity of retrieval methods,etc.,causing the user to face the vast ocean of information, often at a loss,I do not know where to start to obtain the required information,they have to charge a lot of time learning to use various databases, search strategies.This caused a great burden to the user, and a serious impact on resource utilization. To solve this problem, the library at home and abroad are actively working to carry out the integration of digital resources and digital library construction has become a pressing and arduous task.Given the integration of library resources,the importance of development,the integration of digital resources at this stage has become a hot topic in research libraries./n such a cluster of digital libraries, storage media and server specific distribution is not

  5. Psychological Analysis of Public Library Readers in the Environment of Computer Network Resources%计算机网络资源环境下公共图书馆读者心理分析

    Institute of Scientific and Technical Information of China (English)

    梁佳

    2012-01-01

    Public libraries as a nonprofit cultural educational institution,is open for the public,all members of society who use the library resources,library services object.The Public Library of the nature of its readers with a wide range of social and mass characteristics.Understanding of computer network resources and the environment,public library readers psychology,master of readers of different ages tend to provide a basis for public library services.Public Library audience composition and reading motivation and purpose,read the psychological differences.Readers 'psychology,and the corresponding collection of literature data and network information resources services to meet readers' needs to improve the collection of documents and network resource utilization,of great significance for readers of different age groups.The computer network resources and the environment,public library readers psychological to make analysis,to further explore the computer network resources and the environment,how public libraries should better carry out the reader service work.%公共图书馆作为公益性文化教育机构,是面向社会公众开放的,凡是具有利用图书馆资源条件的一切社会成员,都是图书馆服务对象。公共图书馆的性质决定了它的读者具有广泛的社会性和群众性特点。了解计算机网络资源环境下公共图书馆的读者心理,掌握不同年龄读者的阅读倾向,能为公共图书馆读者服务工作提供依据。

  6. JiLin University Library Special Resource%吉林省高校图书馆的特色资源建设--以吉林财经大学为例

    Institute of Scientific and Technical Information of China (English)

    白莉

    2013-01-01

      吉林省高校图书馆特色资源建设是吉林省高校图书馆馆藏建设的重要组成部分,以吉林财经大学为例,提出了吉林省高校图书馆特色资源建设的策略。%Jilin University Library Special Resources is an important part of University Library building in Jilin Province,Jilin University of Finance and Economics,Jilin University Library Special Resources strategy.

  7. Analysis on University Libraries Digital Resources Construction and Service%浅析高校图书馆数字资源建设与服务

    Institute of Scientific and Technical Information of China (English)

    于娟

    2016-01-01

    As the fast development of information technology and internet, paper resources in libraries of colleges and universities have become unsatisifed with the fast development of knowledge and scientiifc efforts. Large amount of paper resources also bring large cost of maintenance. In this condition, digital resources construction with faster updating and lower cost of maintenance is becoming greatly important. Therefore how to well construct digital resources in universities and provide better service for readers has been an important theme to librarians. Only good digital library resources and services can relfect the excellence of digital resources and provide better services to readers.The paper analyzes university libraries digital resources construction and service.%随着信息技术和互联网的飞速发展,高校图书馆中纸质馆藏资源已经渐渐不能满足知识和科研成果突飞猛进的发展需求,同时大量的纸质馆藏资源也带来了较高的维护成本。在这种环境下,更新速度较快、维护成本较低的数字资源建设就显得尤为重要。因此,如何建设好高校数字资源、为广大读者提供更好的数字资源服务,是摆在广大图书馆工作者面前的一个重要课题。只有优质的馆藏数字资源和服务,才能充分体现数字资源的优越性,更好地服务于大众。文章分析了高校图书馆数字资源建设与服务。

  8. 高校图书馆数字资源的采购及评价研究%Study on the Purchase and Evaluation of Digital Resources in University Library

    Institute of Scientific and Technical Information of China (English)

    郑兵

    2015-01-01

    目前,数字资源在高校图书馆馆藏中所占比重越来越大,已经成为图书馆资源的重要组成部分.介绍了高校图书馆数字资源采购的特点与流程,对数字资源的使用评价指标进行了探讨.%At present,the proportion of the digital resources in university library's collection is more and more,and the digital resources have become an important component of library's resources.This paper introduces the features and process of the purchase of university library's digital resources,and probes into the application evaluation indexes of the digital resources.

  9. The National Network of Libraries of Medicine

    Science.gov (United States)

    ... nationwide partnership of health sciences libraries, information centers, public libraries and community-based resource centers. Administered by the ... is available to both health professionals and the public. The Regional Medical Libraries (see map) coordinate a variety of services for ...

  10. Libraries in Pennsylvania: MedlinePlus

    Science.gov (United States)

    ... Doylestown Doylestown Hospital Medical Library 595 West State Street Doylestown, PA 18901 215-345-2606 http://www.dh.org/MedicalLibrary Enola National Sexual Violence Resource Center NSVRC Library 123 North Enola Drive ...

  11. E-learning resource library model based on domain ontology%基于领域本体的电子学习资源库模型

    Institute of Scientific and Technical Information of China (English)

    张沪寅; 张铭洋; 李鑫

    2012-01-01

    随着电子学习系统快速的发展,电子学习资源呈现爆炸式的增长,如何有效地组织海量电子学习资源成为构建高效电子学习系统的重要因素.针对现有资源库在资源组织方面存在的不足,提出了一个基于领域知识本体的电子学习资源库检索模型,该模型利用领域知识来构建领域知识本体库并通过抽取电子学习资源元数据构建元数据库,通过映射关系完成对电子学习资源的语义组织,并在此基础之上构建一个语义检索模型,以有效地解决现有电子学习资源检索中丢失语义背景的问题,使检索结果在查全率、查准率方面有所提高,更加符合用户的需求.%With the rapid development of E-learning system, E-learning resources grow explosively. How to effectively organize E-learning resources is a key factor of constructing efficient E-learning system. Concerning the existing resources organization deficiency of E-learning resource library, this paper proposed an E-learning resource retrieval model based on domain ontology. This model built a domain knowledge library by making use of the domain knowledge and constructed E-learning resources metadata database by extracting resources metadata, realized semantic organization of E-learning resources through mapping relations, and constructed a semantic retrieval model on this basis, in order to effectively solve the problem of the loss of semantic background in the E-learning resource retrieving. The model has also enhanced the recall rate and the precision rate on the retrieval results, and it is more in line with the needs of the users.

  12. Library/vendor relationships

    CERN Document Server

    Brooks, Sam

    2014-01-01

    A view of the mutual dependence between libraries and vendorsAs technology advances, libraries are forced to reach beyond their own resources to find effective ways to maintain accuracy and superior service levels. Vendors provide databases and integrated library systems that perform those functions for profit. Library/Vendor Relationships examines the increasing cooperation in which libraries find they must participate in, and vice versa, with the vendors that provide system infrastructure and software. Expert contributors provide insights from all sides of this unique collaboration, offering

  13. Larger, Higher-level Academic Institutions in the US Do Not Necessarily Have Better-resourced Library Web Teams. A Review of: Connell, Ruth Sara.

    Directory of Open Access Journals (Sweden)

    Suzanne Lewis

    2008-12-01

    -year colleges more likely (91.7% to share a Web team than four-year or above institutions (60.9%. The majority of responding institutions (94.4% used in-house library Web site design, with only 5.6% of respondents outsourcing this task.Nearly half (49% of respondents indicated that library Web design was done by one person and even the larger libraries did not necessarily have larger Web teams. Very few Web team members (4.9% had Web design as their primary role; the majority (83.5% indicated that it was just one component of their job. Web team members from master’s- and doctorate-granting institutions were more likely to have taught themselves Web design, while those from associate, baccalaureate and special focus colleges were more likely to have taken Web design courses. For all respondents, the most commonly listed quality for selection to the Web team was an interest in Web design and the most valued skill for library Web designers was the ability to organise information effectively.Knowledge of Web authoring software and basic HTML coding were the most commonly listed knowledge requirements for Web team members. A significant number of respondents indicated that they or other Web team members did not have access to Web authoring (36.9% and image editing (52% software. Generally (except for two very large institutions, the larger institutions were more likely to use database-driven systems for their library Web sites and the smaller institutions were more likely to use content management systems. Associate’s and special focus colleges were less likely than other types of institutions to use either database driven or content management systems. Associate’s institutions were more likely to achieve ADA (Americans with Disabilities Act Web accessibility compliance. Only 48.6% of respondents utilised usability testing during Web site design.Conclusion – The author expected that institutions providing higher levels of education would have better-resourced Web design and

  14. Library Publishing is Special: Selection and Eligibility in Library Publishing

    OpenAIRE

    Royster, Paul

    2014-01-01

    Traditional publishing is based on ownership, commerce, paid exchanges, and scholarship as a commodity, while library activities are based on a service model of sharing resources and free exchange. I believe library publishing should be based on those values and should not duplicate or emulate traditional publishing. University presses have mixed views of library publishing, and libraries should not adopt those attitudes. Library publishers are not gatekeepers; their mission is dissemination....

  15. University of Delaware Library/Statewide K-12 Partnership Providing Online Resources and Training: UDLib/SEARCH.

    Science.gov (United States)

    Millard, Sandra K.

    UDLib/SEARCH is a unique partnership between the University of Delaware Library and state K-12 education in Delaware. UDLib/SEARCH provides access to 16 full text databases via the Internet on the state network to all Delaware public high schools and middle schools, and training for teachers and librarians. Using $30 million in state funds,…

  16. Paths of Discovery: Comparing the Search Effectiveness of EBSCO Discovery Service, Summon, Google Scholar, and Conventional Library Resources

    Science.gov (United States)

    Asher, Andrew D.; Duke, Lynda M.; Wilson, Suzanne

    2013-01-01

    In 2011, researchers at Bucknell University and Illinois Wesleyan University compared the search efficacy of Serial Solutions Summon, EBSCO Discovery Service, Google Scholar, and conventional library databases. Using a mixed-methods approach, qualitative and quantitative data were gathered on students' usage of these tools. Regardless of the…

  17. Canadian Library Human Resources Short‐Term Supply and Demand Crisis Is Averted, But a Significant Long‐Term Crisis Must Be Addressed. A review of: 8Rs Research Team. The Future of Human Resources in Canadian Libraries February 2005. Edmonton, AB: University of Alberta. 21 February 2007 .

    Directory of Open Access Journals (Sweden)

    Julie McKenna

    2007-03-01

    Full Text Available Objective – To examine the human resources environment in Canadian libraries in order to assess readiness to accommodate change and to identify opportunities for human resources planning. The “8Rs” of the study were defined as recruitment, retirement, retention, remuneration, repatriation, rejuvenation, re‐accreditation, and restructuring.Design – This study was undertaken in three phases over nearly three years through the use a variety of methods including literature review, analyses of existing data (Statistics Canada and library school graduate data, telephone interviews (with senior library administrators, focus groups (with representatives from Canadian Association of Research Libraries, Canadian Urban Libraries Council and Alberta Association of Library Technicians, print surveys (library institutions and web‐based surveys (of professional librarians and paraprofessional library staff.Setting – Canadian libraries that are not component branches of a system, and that employ professional librarians.Subjects – Stage I: 17 senior library administrators participated in telephone interviews and three focus groups were conducted. Stage II: Surveyed library administrators representing institutions. A multi‐stage stratified random sampling technique was used to ensure geographical representation from each of Canada’s provinces and territories. Full census participation was conducted for members of the Canadian Association of Research Libraries and the Canadian Urban Libraries Council. The print survey instrument was distributed to 1,357 subjects; 461 completed surveys were returned (response rate of 34% with results for the total sample accurate within plus or minus 3.8 per centage points, 95 times out of 100. Stage III: Surveyed professional librarians and paraprofessional staff. Multi‐stage random sampling was used to ensure representation of library staff from all library sectors and sufficient sub‐sample sizes. Of the

  18. 高校图书馆数字资源建设刍议%The Discussion of the Construction of the University Library Digital Resource

    Institute of Scientific and Technical Information of China (English)

    王令祎

    2014-01-01

    Summary: the construction of the digital resource has become the most important part of University li-brary's literature resources construction, but there still has many Underlying reasons which put off the development of the construction of digital resources. This paper will discuss and show some ideas on six questions, which are the construction of digital resources, popularization of it, intellectual property protection problems, interlibrary coopera-tion, the construction standards of digital resources, and evaluation system of users.%数字资源建设已经成为高校图书馆文献资源建设的重要组成部分,但许多深层次原因阻碍着高校数字资源建设的脚步。本文从数字资源重复建设、推广普及度、知识产权保护问题、馆际协作、数字资源建设标准和使用评价系统六个方面进行探讨,为问题的解决提出一些思路。

  19. Spectral library searching in proteomics.

    Science.gov (United States)

    Griss, Johannes

    2016-03-01

    Spectral library searching has become a mature method to identify tandem mass spectra in proteomics data analysis. This review provides a comprehensive overview of available spectral library search engines and highlights their distinct features. Additionally, resources providing spectral libraries are summarized and tools presented that extend experimental spectral libraries by simulating spectra. Finally, spectrum clustering algorithms are discussed that utilize the same spectrum-to-spectrum matching algorithms as spectral library search engines and allow novel methods to analyse proteomics data.

  20. Library service to dental practitioners.

    OpenAIRE

    Ashin, E R

    1983-01-01

    Dental school libraries offer resources of value to dental practitioners, but do not always consider practitioners to be primary clientele. A survey was conducted among the sixty U.S. dental school libraries to examine policies and attitudes toward service to practitioners. Although library use by dentists is estimated to be low, most libraries are willing to serve them as long as it does not reduce the libraries' ability to assist students and faculty. Of the respondents, 57% replied that th...

  1. Novel SSR markers from BAC-end sequences, DArT arrays and a comprehensive genetic map with 1,291 marker loci for chickpea (Cicer arietinum L..

    Directory of Open Access Journals (Sweden)

    Mahendar Thudi

    Full Text Available Chickpea (Cicer arietinum L. is the third most important cool season food legume, cultivated in arid and semi-arid regions of the world. The goal of this study was to develop novel molecular markers such as microsatellite or simple sequence repeat (SSR markers from bacterial artificial chromosome (BAC-end sequences (BESs and diversity arrays technology (DArT markers, and to construct a high-density genetic map based on recombinant inbred line (RIL population ICC 4958 (C. arietinum×PI 489777 (C. reticulatum. A BAC-library comprising 55,680 clones was constructed and 46,270 BESs were generated. Mining of these BESs provided 6,845 SSRs, and primer pairs were designed for 1,344 SSRs. In parallel, DArT arrays with ca. 15,000 clones were developed, and 5,397 clones were found polymorphic among 94 genotypes tested. Screening of newly developed BES-SSR markers and DArT arrays on the parental genotypes of the RIL mapping population showed polymorphism with 253 BES-SSR markers and 675 DArT markers. Segregation data obtained for these polymorphic markers and 494 markers data compiled from published reports or collaborators were used for constructing the genetic map. As a result, a comprehensive genetic map comprising 1,291 markers on eight linkage groups (LGs spanning a total of 845.56 cM distance was developed (http://cmap.icrisat.ac.in/cmap/sm/cp/thudi/. The number of markers per linkage group ranged from 68 (LG 8 to 218 (LG 3 with an average inter-marker distance of 0.65 cM. While the developed resource of molecular markers will be useful for genetic diversity, genetic mapping and molecular breeding applications, the comprehensive genetic map with integrated BES-SSR markers will facilitate its anchoring to the physical map (under construction to accelerate map-based cloning of genes in chickpea and comparative genome evolution studies in legumes.

  2. 当前高校图书馆人力资源管理问题研究%Study on Human Resource Management of Current University Library

    Institute of Scientific and Technical Information of China (English)

    刘宏

    2012-01-01

    Taking knowledge-based economy environment as the background, the thesis discussed the current status of human resources in university libraries and the problems, and made countermeasures and proposals for human resources optimization from the concept, planning, environment, configuration, training, performance appraisal, culture and other aspects.%以知识经济环境下为背景,讨论了当前高校图书馆人力资源现状及存在的问题,提出了从观念、规划、环境、配置、培训、绩效考核、文化等环节优化人力资源的对策建议.

  3. Learners with Low Self-Efficacy for Information Literacy Rely on Library Resources Less Often But Are More Willing to Learn How to Use Them

    Directory of Open Access Journals (Sweden)

    Dominique Daniel

    2014-09-01

    Full Text Available Objectives – To determine whether there is a relationship between self-efficacy (i.e., confidence regarding information literacy skills and self-efficacy for distance learning; and to compare the use of electronic resources by high and low information literacy self-efficacy distance learners and their interest in learning more about searching. Design – Online survey. Setting – A small public university in the United States of America. Subjects – Undergraduate and graduate students enrolled in one or more online courses. Most respondents were in their twenties, 76% were female, 59% were undergraduates, and 69% were full time students. Methods – Students were asked six demographic questions, eight questions measuring their self-efficacy for information literacy, and four questions measuring their self-efficacy for online learning. All self-efficacy questions were adapted from previous studies and used a one to five Likert scale. The response rate was 6.2%. Correlational analysis was conducted to test the first two hypotheses (students who have higher self-efficacy for information seeking are more likely to have higher self-efficacy for online learning and for information manipulation. Descriptive analysis was used for the remaining hypotheses, to test whether students who have higher information literacy self-efficacy are more likely to have high library skills (hypothesis three and are more interested in learning about how to use library resources (hypothesis four. Among respondents high information literacy self-efficacy and low self-efficacy groups were distinguished, using the mean score of information literacy self-efficacy. Main Results – There was a significant correlation between self-efficacy for information seeking and self-efficacy for online learning (r = .27, as well as self-efficacy for information manipulation (r = .79. Students with high information seeking self-efficacy were more likely to use library databases (28

  4. 图情资源发现系统的研究与实现*%Research and Implementation of Libraries' Resource Discovery System

    Institute of Scientific and Technical Information of China (English)

    王灏; 张正锋; 冯巍

    2013-01-01

      资源发现系统是一种面向图书情报领域的垂直搜索引擎。文章从图情机构馆藏和服务的问题入手,介绍了资源发现系统的概念和技术路线,并以“中国学术搜索网”为例,介绍了该平台在资源发现服务中的总体设计、关键技术、核心功能和服务方式,旨在帮助图书馆等单位在产品技术选型和建设过程中提供可能的参考依据。%Resource discovery system is a kind of vertical search engine focused on library and information science. For the general problem in libraries and information centers, this article gave a brief introduction of the concept and main technologies. With the example of"Chinese Academic Search,"it has provided some practical details about the design, main function and service model of resource systems in order to provide suggestions for others' purchase or construction of discovery service if possible.

  5. A conditional piggyBac transposition system for genetic screening in mice identifies oncogenic networks in pancreatic cancer.

    Science.gov (United States)

    Rad, Roland; Rad, Lena; Wang, Wei; Strong, Alexander; Ponstingl, Hannes; Bronner, Iraad F; Mayho, Matthew; Steiger, Katja; Weber, Julia; Hieber, Maren; Veltkamp, Christian; Eser, Stefan; Geumann, Ulf; Öllinger, Rupert; Zukowska, Magdalena; Barenboim, Maxim; Maresch, Roman; Cadiñanos, Juan; Friedrich, Mathias; Varela, Ignacio; Constantino-Casas, Fernando; Sarver, Aaron; Ten Hoeve, Jelle; Prosser, Haydn; Seidler, Barbara; Bauer, Judith; Heikenwälder, Mathias; Metzakopian, Emmanouil; Krug, Anne; Ehmer, Ursula; Schneider, Günter; Knösel, Thomas; Rümmele, Petra; Aust, Daniela; Grützmann, Robert; Pilarsky, Christian; Ning, Zemin; Wessels, Lodewyk; Schmid, Roland M; Quail, Michael A; Vassiliou, George; Esposito, Irene; Liu, Pentao; Saur, Dieter; Bradley, Allan

    2015-01-01

    Here we describe a conditional piggyBac transposition system in mice and report the discovery of large sets of new cancer genes through a pancreatic insertional mutagenesis screen. We identify Foxp1 as an oncogenic transcription factor that drives pancreatic cancer invasion and spread in a mouse model and correlates with lymph node metastasis in human patients with pancreatic cancer. The propensity of piggyBac for open chromatin also enabled genome-wide screening for cancer-relevant noncoding DNA, which pinpointed a Cdkn2a cis-regulatory region. Histologically, we observed different tumor subentities and discovered associated genetic events, including Fign insertions in hepatoid pancreatic cancer. Our studies demonstrate the power of genetic screening to discover cancer drivers that are difficult to identify by other approaches to cancer genome analysis, such as downstream targets of commonly mutated human cancer genes. These piggyBac resources are universally applicable in any tissue context and provide unique experimental access to the genetic complexity of cancer.

  6. The Egyptian Web Logs as an Information Resource with a Special Indication to Library and Librarian's Blogs

    OpenAIRE

    Shayma' Ismael Abbas Ismael

    2007-01-01

    An article is discussing The Egyptian Web Logs ,library blogs and librarian's blogs at the world wide web as one of information electronic sources so this article is answering on some questions. what is conception of blogs?, What is historic development and origin of blogs?, What are reasons to fame and spread blogs between internet employees ?, what is blogging software to create blog?, what is directory and search engine of blogs?, what is characteristics of successful blogs? and concen...

  7. Share and share alike: encouraging the reuse of academic resources through the Scottish electronic Staff Development Library

    Directory of Open Access Journals (Sweden)

    Lorna M. Campbell

    2001-12-01

    Full Text Available The Scottish electronic Staff Development Library (http://www.sesdl.scotcit.acuk is an ongoing collaborative project involving the Universities of Edinburgh, Paisley and Strathclyde which has been funded by SHEFC as part of their current ScotCIT Programme (http:llwww.scotcit.ac.uk. This project is being developed in response to the increasing demand for flexible, high-quality staff development materials.

  8. The Mining and Expanding of University Library Resources Based on WeChat Platform%基于微信平台的高校图书馆资源的挖掘与拓展

    Institute of Scientific and Technical Information of China (English)

    沈海燕

    2015-01-01

    运用微信平台挖掘高校图书馆资源,可推动图书馆资源的扩展,从而以最快速、最精准、最有效的网络传播方式把图书馆资源传递出去。简要阐述了微信移动互联网的特征及优势,分析了挖掘高校图书馆资源的需求以及高校使用微信的必要性,探讨了在微时代拓展高校图书馆资源的方法与途径。%Mining university library resources by using WeChat platform can promote the expansion of library resources, and transmit library resources with the the most rapid, most accurate and most effective network transmission mode. This paper briefly expounds the features and advantages of WeChat Mobile Internet, analyzes the demands for mining university library resources and the necessity of using WeChat in university, and probes into the methods and paths for expanding university library resources in micro age.

  9. Featured Library: Parrish Library

    OpenAIRE

    Kirkwood, Hal P, Jr

    2015-01-01

    The Roland G. Parrish Library of Management & Economics is located within the Krannert School of Management at Purdue University. Between 2005 - 2007 work was completed on a white paper that focused on a student-centered vision for the Management & Economics Library. The next step was a massive collection reduction and a re-envisioning of both the services and space of the library. Thus began a 3 phase renovation from a 2 floor standard, collection-focused library into a single floor, 18,000s...

  10. An active piggyBac-like element in Macdunnoughia crassisigna

    Institute of Scientific and Technical Information of China (English)

    Min Wu; Zhi-Chan Sun; Chun-Lin Hu; Gu-Feng Zhang; Zhao-Jun Han

    2008-01-01

    In this paper,a highly conserved piggyBac-like sequence.designated as McrPLE was cloned from a lepidopteran insect,Macdunnoughia crassisigna.It is 2 472 bp long in full length with a single open reading frame and encodes a 595 amino acid transPOsase.It shares identical terminal and sub-terminal repeats with T. ni IFP2 and iS flanked by the typical TTAA target-site duplications.Alignment and phylogeneric analysis revealed that McrPLE had greater than 99.5%identity and appeared to be the closest one in phylogeny to IFP2 among the PLEs so far found in various species.Plasmid.based excision and transposition assay proved it Was mobile in cell culture.Otherwise.McPLE element and all other highly conserved IFP2 sequences reported previously were found to share three corrution nucleotide substitutions.This suggests that the original IFP2 may be a related variant of a predecessor element that became widespread.The existence of nearly identical piggyBac sequence in reproductively isolated species Was thought also a strong indication of horizontal transmission,which raises important considerations for the stability and practical use of piggyBac transformation vectors.

  11. Cypriot libraries

    OpenAIRE

    John F. Harvey

    1982-01-01

    Describes the current state of librarianship and bibliography in Cyprus, with separate sections for the Greek and Turkish sectors. Although there is no national library in the Greek sector there are 5 types of public library: Nicosia public library; Limassol, Larnaca and Paphos public libraries; community libraries; mobile libraries; and foreign cultural centre libraries. Schools and colleges in the Greek centre are well provided with libraries and most government departments sponsor special ...

  12. A BAC clone of MDV strain GX0101 with REV-LTR integration retained its pathogenicity

    Institute of Scientific and Technical Information of China (English)

    SUN AiJun; LAWRENCE Petherbridge; ZHAO YuGuang; LI YanPeng; NAIR Venugopal K; CUI ZhiZhong

    2009-01-01

    The complete genome of Marek's disease virus (MDV) strain GX0101,which was integrated with the LTR sequences of REV,was cloned in Escherichia coli as a bacterial artificial chromosome (BAC).BAC vector sequences were introduced into the US2 locus of the MDV genome by homologous recombination.The viral DNA containing the BAC vector was used to transform Escherichia coli strain of DH10B.Then the recombinant virus was successfully rescued by transfection of the recombinant BAC DNA into primary chicken embryo fibroblast (CEF).This BAC viral clone was named bac-GX0101.When the reconstituted virus was inoculated into 1-day-old birds,visceral tumors could be detected as early as 62 d post infection.There was no difference in growth ability and pathogenicity to birds between the BAC derived virus and its parental virus.The BAC derived virus maintained its oncogenicity and immunosuppressive effects.In conclusion,the complete genome of GX0101 strain was successfully cloned into BAC and the infectious clone was rescued.With the powerful BAC manipulation system,the infectious clone will provide a useful tool for further understanding the functional roles of the inserted REV-LTR sequence in the GX0101 strain of MDV.

  13. 开放获取对图书馆信息资源建设的影响%Impact of Open Access on Information Resources Construction in Library

    Institute of Scientific and Technical Information of China (English)

    麻思蓓

    2015-01-01

    介绍了国内外开放获取最新进展。通过对比分析开放获取资源和印刷型期刊及数据库的特点和优劣势,阐述了开放获取对图书馆信息资源建设战略、馆藏结构、组织揭示和共建共享所带来的影响。从我国情况看,今后一段时间,开放资源、数字化印本资源、数据库将同时作为馆藏共存,亟需探索和研究适合远程数字型网络资源的组织规范,信息资源的开放和共享是大势所趋。%Introduced latest progress on open access at home and abroad. By comparing and analyzing the advantages and disadvantages of OA resources , printing periodicals and databases, this paper expounds the profound impact of OA on information resource construction, collection structure, resource description and knowledge sharing in library. According to the speciifcs in China, open resources will coexist with digital print documents and databases in the next period of time. It’s urgent need to explore and research more suitable cataloging rules for internet digital resources. Openning and sharing of information resources is coming trend.

  14. Global Warming's Library Challenge

    Science.gov (United States)

    Meyer, Jennifer

    2008-01-01

    Like every institution that uses energy, consumes resources, and engages in construction or renovation, libraries have an impact on the environment and on the critical problem of climate change. Taking action to protect library collections is not only an idealistic professional goal but also a very practical one. Disaster preparation measures and…

  15. Academic Libraries in Japan

    Science.gov (United States)

    Cullen, Rowena; Nagata, Haruki

    2008-01-01

    Academic libraries in Japan are well resourced by international standards, and support Japan's internationally recognized research capability well, but there are also ways in which they reflect Japan's strong bureaucratic culture. Recent changes to the status of national university libraries have seen a new interest in customer service, and…

  16. The Thinking of Print Resource Development in a University Library in the Digital Age%数字化时代高校图书馆纸质馆藏资源建设的思考

    Institute of Scientific and Technical Information of China (English)

    彭少杰; 岳国庆

    2012-01-01

    图书馆网络化、数字化的发展,给馆藏结构及服务模式带来了深刻的变革。对数字化时代高校图书馆纸质馆藏资源建设进行了重新思考,重点阐述了新形势下馆藏结构的优化、学科分布、专业特色、开发利用以及纸质文献的共建共享等问题。%The development of interact and digital resources in libraries brings the revolutionary changes of the library collection structures and library services. The author of this article rethinks the establishment of print resources in a university library in the digital age. It introduces the issue of the optimization in the library collection structure, subject distribution, the specialties of the library, and the utilization and communal building and sharing of print resources in this new trend.

  17. News from the Library

    CERN Multimedia

    CERN Library

    2010-01-01

    The LHC Library to be merged with the Central Library. Not everyone knows that CERN Scientific Information Service currently counts three physical libraries on site. The Central Library is located in Building 52 and there are two satellite libraries located respectively in building 30 (the LHC Library) and in building 864 on Prévessin site (the SPS Library). Moreover, the Legal Service Library is located in Building 60. In the past, there have been at CERN up to 6 satellite libraries; they were essential at a time when information was only in paper form and having multiple copies of documents located in several places at CERN was useful to facilitate scientific research. Today, this need is less critical as most of our resources are online. That is why, following a SIPB (Scientific Information Policy Board) decision, the collections of the LHC Library will be merged this summer with the Central collection. This reorganization and centralization of resources will improve loan services. The SP...

  18. 教学资源库规范化构建研究%Standardized Construction of Teaching Resource Library

    Institute of Scientific and Technical Information of China (English)

    陈萱华; 张玲

    2011-01-01

    In order to achieve the resources sharing and reusing by solving the resources' exchange, inquiry and extraction,a standardized description is proposed for the teaching resources from two aspects of metadata and camponent. Using triad to described the component resources and adopts four parts to package the resources content. The storage patterns of distributed storage centralized sharing is proposed by comparing the advantages and disadvantages of centralized and distributed storage.The results show that describing the teaching resources based on the component is advantageous in teaching resources reconstruction and reuse.%为了解决资源的交换,查询、提取,实现资源共享和重用的目的,从元数据和构件2个层面对教学资源进行规范化地描述.采用三元组描述构件资源,对资源进行内容包装.比较集中式和分布式存储的优缺点提出分布存储、集中共享的存储模式.结果表明基于构件描述教学资源的思想,有利于教学资源的重构和复用.

  19. Research on digital teaching resource library mode in distance education based on cloud computing%基于云计算的远程教育数字化教学资源库模式研究

    Institute of Scientific and Technical Information of China (English)

    刘晓丹; 蒋漪涟

    2015-01-01

    In order to realize the sharing of learning resources in the distance education and meet the needs of dif -ferent learners , it analyzes the digital teaching resource library in distance education from the cloud computing technology , proposes the teaching resource library model and the key technology , discusses the construction of teaching resource library mode , establishes the logical structure and platform structure of resource library mode , points out the broad prospect of the application of cloud computing technology in the construction of distance edu -cation resources .%为实现远程教育中的学习资源共享,满足不同学习者对资源的需求,对远程教育数字化教学资源库模式进行了研究. 从云计算技术、教学资源库模式、关键技术3个方面探讨了教学资源库的模式建设,阐述了资源库模式的逻辑架构和平台架构,指出了云计算技术在远程教育资源库建设中应用的广阔前景.

  20. Iowa DNR - NRGIS Library

    Data.gov (United States)

    Iowa State University GIS Support and Research Facility — The Natural Resources Geographic Information System (NRGIS) Library is a Geographic Information System (GIS) repository developed and maintained by the GIS Section...

  1. Analysis of Optimization on Paper Resources and Digital Resources in Library%图书馆纸质资源与电子资源优化配置分析

    Institute of Scientific and Technical Information of China (English)

    尹爱兰

    2012-01-01

    This text fast summaries budget line, indifference curves and utility maximization of economy, and whose are explained in library; then analyzes the optimization of paper resources and digital resources of fixed expenses in library, finally, lists the corresponding function of the type and comes up with a way to find the optimal solution.%在预算线、无差异曲线及效用最大化等相关经济学理论知识的基础上对图书馆采购预算线、读者无差异曲线及读者效用最大化进行了诠释,接着从读者对信息需求的角度出发,对图书馆在有限采购经费的前提下如何优化配置纸质资源和电子资源进行了详细的分析,最后再列出相应函数关系式并得出一种求最优解的方法。

  2. Co-construction and Sharing of Public Libraries ’ Exhibition Resources---Taking the Wenyuan Exhibition of Shanxi Library as an Example%公共图书馆展览资源的共建共享--以山西省图书馆“文源视界”为例

    Institute of Scientific and Technical Information of China (English)

    李红; 石焕发

    2014-01-01

    以山西省图书馆“文源视界”为例,概述了山西省公共图书馆展览工作现状,探讨了山西省公共图书馆展览资源共建共享的途径。%Taking the Wenyuan Exhibition of Shanxi Library as an example, this paper reviews the current status of the exhibition work of public libraries in Shanxi Province, and probes into the paths for the co-construction and sharing of exhibition resources of public libraries in Shanxi Province.

  3. Enhancing Digital Information Access in Public Libraries

    OpenAIRE

    Ayers, Kathleen; Liu, Yan Quan

    2006-01-01

    To explore the impact of public digital library resources on urban residents, to elucidate the current usage patterns of public digital library resources/services and levels of satisfaction with the resources/content in urban digital libraries and make recommendations as to steps that would improve service to this population.

  4. 基于内部云存储的图书馆数据资源存储研究%Design for Library Data Resources Storage System Based on Internal Cloud Storage

    Institute of Scientific and Technical Information of China (English)

    乔杨

    2012-01-01

    Reliable storage environment is the basis for library digital resources providing efficient services for users. Based on the discussion of library digital resources storage different technologies, cloud storage connotation and applications in library are analyzed in this paper. According to the characteristics of library digital resources, a storage architecture based on internal cloud storage for library digital resources is designed. And the hierarchical structure, the methods of access control, expansion and deployment are detailed.%可靠存储环境是图书馆数字资源为用户提供高效服务的保障。在阐述图书馆数据资源存储技术基础上,分析了云存储内涵及在图书馆中的应用,探讨基于内部云存储的图书馆数字资源数据存储方案,详细分析了其层次架构、访问控制、扩展和部署方法。

  5. Performance of BAC process for treatment of micro-polluted water

    Institute of Scientific and Technical Information of China (English)

    WANG Chen; MA Fang; SHAN Dan; YANG Ji-xian; Chang Chein-chi

    2008-01-01

    Biological activated carbon (BAC) has been developed on the granular activated carbon by immobi-lization of selected and acclimated species of bacteria to treat the micro-polluted water. The BAC removal effi-ciencies for nitrobenzene, permanganate index, turbidity and ammonia were investigated. Effects of shock load-ing and SEM (Scanning Electron Microscope) observation on BAC were studied. Baekwashing and its intensity of BAC were also discussed. The results showed that BAC took short time to start up and recover to the normal condition after shock loading. The shock loading studies showed that the removal efficiency of BAC was not completely inhibited even at high concentration of nitrobenzene. Backwashing performed once every 10- 20 d,or an average of 15 d. Backwashing intensity was 12-14 L/( s·m2) with air and 3-4 L/( s·m2) with water.

  6. Thoughts on Bibliographic Information Resources Construction and Sharing of University Libraries in Guangxi%对广西高校图书馆信息资源共建共享的思考

    Institute of Scientific and Technical Information of China (English)

    胡可东

    2012-01-01

    通过对广西高校图书馆信息资源共建共享的可行性及制约因素进行分析,并提出了广西高校图书馆信息资源共建共享的对策。%This paper analyses the feasibility and barriers of realizing Bibliographic Information Resources Construction and Sharing of University Libraries in Guangxi,measures to realize literature information resources construction and sharing of university libraries in Guangxi are proposed.

  7. Thinking on Strategies of Information Resource Constructions of University Libraries under Network Environment%网络环境下高校图书馆信息资源建设的策略思考

    Institute of Scientific and Technical Information of China (English)

    李萍

    2011-01-01

    Based on the new changes of information resource construction of library under network environment, this paper analyses the existing problems, and puts forward the strategies of information resource construction of university library under network envir%立足于网络环境下图书馆信息资源建设出现的新变化,对其存在的问题进行了分析,提出了网络环境下高校图书馆信息资源建设的策略。

  8. 论新信息环境下中小型图书馆信息资源建设%Discussion on Information Resources Construction of Small and Medium-sized Libraries in the New Information Environment

    Institute of Scientific and Technical Information of China (English)

    叶菁

    2015-01-01

    论述了新信息环境下中小型图书馆信息资源建设的定位,探讨了新信息环境下中小型图书馆信息资源建设的有效对策.%This paper discusses the positioning of information resources construction of small and medium-sized libraries in the new information environment, and probes into some effective countermeasures for small and medium-sized libraries to carry out the information resources construction in new information environment.

  9. On the Application of Flexible Management in Human Resource Management of Library%柔性管理在图书馆人力资源管理中的应用研究

    Institute of Scientific and Technical Information of China (English)

    袁峰; 叶丽红

    2012-01-01

    在简述柔性管理涵义的基础上,针对图书馆人力资源管理现状,提出图书馆在人力资源管理中应用柔性管理的策略。%Based on the description of the meaning of flexible management,this paper puts forward the strategies of the application of flexible management in library human resource management according to the current situation of library human resource management.

  10. 双因素理论在图书馆人力资源管理中的应用探讨%The Application of Dual-Factor Theory in Library Human Resource Management

    Institute of Scientific and Technical Information of China (English)

    胡忠华

    2011-01-01

    The application of dual-factor theory in library human resource management can effectively motivate staff and improve management efficiency. This paper discusses the meaning of dual-factor theory and its application in library human resource management.%将双因素理论运用于图书馆人力资源管理中,能有效地激励员工,提高管理效率。阐述了双因素理论的含义,对图书馆如何运用双因素理论进行人力资源管理进行了探讨。

  11. 论新信息环境下中小型图书馆信息资源建设%Discussion on Information Resources Construction of Small and Medium-sized Libraries in the New Information Environment

    Institute of Scientific and Technical Information of China (English)

    叶菁

    2015-01-01

    This paper discusses the positioning of information resources construction of small and medium-sized libraries in the new information environment, and probes into some effective countermeasures for small and medium-sized libraries to carry out the information resources construction in new information environment.%论述了新信息环境下中小型图书馆信息资源建设的定位,探讨了新信息环境下中小型图书馆信息资源建设的有效对策.

  12. 烟叶表面高效降解蛋白质芽孢杆菌的分离筛选及其鉴定%Diagnosis to Utilization rate of Collection Resources of the University Library in National Minority Area——Take the Library of the Kashgar Teachers College as an Example

    Institute of Scientific and Technical Information of China (English)

    马英

    2012-01-01

    Library utilization rate of the library embodies the being value of the university library.Therefore,how to improve the utilization of library is common concern,how to improve the utilization rate of the library is also a problem to be solved urgently.This article have investigated utilization rate of the library of Kashi Teacher's College,and analyzed the utilization rate of minority teachers college library,put forward the measures to use library resources reasonably.%利用芽孢杆菌耐高温的特性,从烟叶表面分离筛选并得到1株可高效降解蛋白质的菌株F-Y-11,其蛋白质降解圈直径与菌落直径比值(HC值)为5.50,蛋白酶活力为25.67 U。通过形态学特征及生理生化特征鉴定菌株F-Y-11为芽孢杆菌属。将其制成菌剂对烟丝进行发酵,测得蛋白质降解率为29.32%。

  13. Higher vocational college library information resource construction%高职院校图书馆网络信息资源建设研究

    Institute of Scientific and Technical Information of China (English)

    魏蔚; 易平

    2015-01-01

    The network information resources construction is a necessary choice to adapt the time request and reader demand, it can meet the needs of the readers of electronic books, improve the literacy of the readers. The network information resources construction in the current higher vocational coleges face a shortage of funds, lack of talent, etc, but also has the advantages of the investment, the state attaches great importance to the development of higher vocational. Therefore, higher vocational coleges should take positive measures to adapt to the requirement of information era, efforts to do a good job of higher vocational colege library information resources construction.%网络信息资源建设是适应时代要求和读者需求的必然选择,它可以满足读者对电子图书的需要,提高读者的文化素养。当前高职院校图书馆网络信息资源建设面临资金短缺,人才匮乏等困境,同时也有投入小、国家重视高职发展等优势。因此,高职院校要采取有利措施适应信息化时代的要求,努力做好高职院校图书馆网络信息资源建设工作。

  14. Research on Colleges and Universities’Serving Local Cultural Development by Their Library Resources---Taking Anhui Science and Technology University Library as an Example%高校馆藏资源服务地方文化建设管窥--以安徽科技学院图书馆为例

    Institute of Scientific and Technical Information of China (English)

    王友莲; 卫胜

    2014-01-01

    高校馆藏资源提供利用是建设地方文化的重要保障条件之一。高校图书馆在弘扬和促进地方文化建设方面要充分发挥丰富的馆藏资源优势并定位好服务角色。2006年起安徽科技学院图书馆主动对接地方文化建设,面向社会读者陆续开放其馆藏资源。总结安徽科技学院馆藏资源服务地方文化建设的经验如下:转变思想,树立服务社会的理念;利用馆藏资源主动对接地方文化发展的需要;面向社会开放馆藏资源;实现了高校馆藏资源与地方文化建设的良性循环与互动。%The availability of library resources of colleges and universities is one of the important guarantees of local cultural development. Their libraries should give full play to the advantages of their rich library resources and determine their service role in the promotion of local cultural development. Since 2006, the library of Anhui Science and Technology University has been volunteering to connect itself with local cultural development and making its library resources available for social readers. Through the author summarized experience of it universities in their serving local cultural development with library resources, hoping to provide reference for relevant practices and studie:Change their thinking, carding community service concept, Use library resources to connect with the needs of local cultural development. Open society library resources; Achieve a virtuous cycle of interaction with the local university library resources and cultural construction.

  15. Sequenced BAC anchored reference genetic map that reconciles the ten individual chromosomes of Brassica rapa

    OpenAIRE

    Park Beom-Seok; Jin Mina; Van Nguyen Dan; Hossain Md; Lee Seo; Hong Chang; Bae Jina; Choi Su; Kim HyeRan; Bang Jea-Wook; Bancroft Ian; Lim Yong

    2009-01-01

    Abstract Background In view of the immense value of Brassica rapa in the fields of agriculture and molecular biology, the multinational Brassica rapa Genome Sequencing Project (BrGSP) was launched in 2003 by five countries. The developing BrGSP has valuable resources for the community, including a reference genetic map and seed BAC sequences. Although the initial B. rapa linkage map served as a reference for the BrGSP, there was ambiguity in reconciling the linkage groups with the ten chromos...

  16. Digital Libraries on the Internet.

    Science.gov (United States)

    Sharon, Taly; Frank, Ariel J.

    This paper discusses digital libraries on the Internet. The resource repository hierarchy, consisting of two major paradigms, search engines (SEs) and digital libraries, is presented. SEs are classified into three categories: basic-SE, directory, and meta-SE. The following six major characteristics of a library are summarized: collection of data…

  17. Construction and Characterization of Three Wheat Bacterial Artificial Chromosome Libraries

    Directory of Open Access Journals (Sweden)

    Wenjin Cao

    2014-11-01

    Full Text Available We have constructed three bacterial artificial chromosome (BAC libraries of wheat cultivar Triticum aestivum Wangshuibai, germplasms T. monococcum TA2026 and TA2033. A total of 1,233,792,170,880 and 263,040 clones were picked and arrayed in 384-well plates. On the basis of genome sizes of 16.8 Gb for hexaploid wheat and 5.6 Gb for diploid wheat, the three libraries represented 9.05-, 2.60-, and 3.71-fold coverage of the haploid genomes, respectively. An improved descending pooling system for BAC libraries screening was established. This improved strategy can save 80% of the time and 68% of polymerase chain reaction (PCR with the same successful rate as the universal 6D pooling strategy.

  18. Thinking on Constriction of Literature Resource System in Libraries%对图书馆文献资源体系建设的思考

    Institute of Scientific and Technical Information of China (English)

    黄芳

    2009-01-01

    对图书馆馆藏文献资源构成进行合理分类,同时对其特点进行科学分析,提出调整馆藏发展政策、建立特色化馆藏体系和优化采访人员素质等3大措施来优化图书馆文献资源体系建设.%Carrying out reasonable classification of literature collection and analyzing the characteristics scientifically, the paper points out adjusting development policies for collection, constructing characteristic collection system and optimizing the quality of acquisition librarian, which would play an positive role in improving the construetion of literature resource system in libraries.

  19. Vocational Teaching Resource Library based on Ubiquitous Learning%基于泛在学习的高职专业教学资源库研究

    Institute of Scientific and Technical Information of China (English)

    范效亮; 杨卫军

    2014-01-01

    随着移动互联技术的快速发展和教育信息化的推进,利用移动互联网络的泛在学习成为人们学习的一种趋势。适应新技术给人们带来的学习方式的变革,基于泛在学习的需求建设高职专业教学资源库,推进专业教学改革,提高教学质量。%With the rapid development of mobile Internet technology and the advance of information technology education, the use of mobile Internet ubiquitous learning become a trend that people learn. Adapt to changes in technology to bring new ways of learning, construction of vocational teaching resource library needs-based Ubiquitous Learning, promote the teaching reform, improve the quality of teaching.

  20. Research on Henan University Libraries Multimedia Resource Sharing Model%多媒体环境下河南高校图书馆资源共享模式研究

    Institute of Scientific and Technical Information of China (English)

    吴箐

    2015-01-01

    Development of multimedia has changed the speed and way of people to obtain information,the fundamental driving force to achieve university library resource sharing mode is the innovation in the media en⁃vironment. A single category of library resources cannot meet the need of readers to get considerable informa⁃tion. The existing problems in university libraries resource sharing in Henan were analyzed under multimedia environment,making efforts to construct reasonable information resources sharing mode, so asto improve the utili⁃zation of library resources,.%多媒体的发展改变了人类获取信息的速度和方式,媒介环境的变革是高校图书馆实现资源共享模式的根本动力。单一类别的图书馆资源无法满足读者获取大量信息的需求。本文以多媒体环境为背景,分析了河南高校图书馆在资源共享时出现的问题,试图合理建构信息资源共享模式,提高图书馆的资源利用率。

  1. Efficacy of Indexing and Abstracting Services for the Dissemination of Agricultural Information Resources in the Institure for Agricultural Research Library, Ahmadu Bello University, Zaria

    Science.gov (United States)

    Gabriel, KASA, M.

    2012-10-01

    The efficacy of Indexing and Abstracting service for effective organization, storage and retrieval of information resources for agricultural research in Ahmadu Bello University, Zaria necessitated examining the situation in Agricultural Library, Institute for Agricultural Research, Samaru. The study examines the processes, awareness and problems militating against the effective exploitation of the indexing and abstracting services in the Agricultural library established in 1975. The study was conducted ex post facto, data collected span from 2006 ñ 2010. Total sample sizes of 752 patrons and 20,236 intellectually indexed and abstracted resources were involved in the study. Data collected were subjected to descriptive and inferential statistics. The result revealed that a total of 644 articles were indexed and abstracted, 35% of these was done in 2010. Results for awareness show 452 (60.11%) to be aware in 2008. A total 584 articles were indexed and abstracted from which 167 (28.59%) was retrieved in 2006. Patrons, 270 (35.90%) attributed the poor use of the service to assumption it is a referral unit. The hypothesis testing revealed that there is significant association between articles indexed and abstracted with information consulted by patrons (?2cal,100.31>?2tab,9.488) at 5% level of probability and df, 4. In conclusion, enormous documents on Nigerian agriculture are indexed and abstracted in the unit, implying that the service is desirous and consistent. The study recommends that the unit should explore the use of modern technology, employ a permanent subject specialist, train and retrain the unit staff as well as intensify it general orientation campaigns to focus on awareness and use of the indexing and abstracting services.

  2. Research on Fund Allocation of Academic Libraries Information Resource Development%高校图书馆信息资源建设经费配置研究

    Institute of Scientific and Technical Information of China (English)

    徐桂菊

    2011-01-01

    高校图书馆信息资源建设经费是高校图书馆建设与发展的经济基础,其配置的方法、模式以及配置比例直接关系到高校图书馆信息资源的建设发展,其配置过程受到图书馆文献经费使用的历史数据、读者的需求变化、学校学科的发展目标、文献价格自然增长等因素的影响,高校应综合考虑这些因素的影响,选用正确的方法,合理配置信息资源建设经费,使高校有限的资金发挥最大的效益,满足高校读者的阅读需求,促进高校信息资源建设的良性发展.%The fund allocation in the construction of information resources is the economic base for the construction and development of academic libraries. Its allocation method, mode and proportion directly relate to the development of academic libraries. Its allocation process is influenced by history data of fund allocation, changes of readers' need, development goals of academic disciplines, and the reasonable growing of literature cost. Universities should consider all the influent factors and choose the right way to allocate the fund properly, maximize the benefits of the fund, cater for the needs of university readers and promote the positive development of university information resources.

  3. Construction and characterization of a bacterial artificial chromosome library of thermo-sensitive genic male-sterile rice 5460S

    Institute of Scientific and Technical Information of China (English)

    邱芳; 金德敏; 伏健民; 张超良; 谢纬武; 王斌; 杨仁崔; 张洪斌

    1999-01-01

    In order to develop a detailed physical map of the thermo-sensitive genie male-sterile (TGMS) gene-encompassing region and finally clone the TGMS gene, a high-quality rice bacterial artificial chromosome (BAC) library from TGMS rice 5460S was constructed. The method of constructing BAC library was examined and optimized. The 5460S library consists of 19 584 BAC clones with an average insert size of 110 kb, which represents about 5 times rice haploid genome equivalents. Rice inserts of up to 140 kb and 250 kb were isolated and appeared stable after 100 generations of serial growth. Hybridization of BAC clones with mitochondrial and chloroplastic genes as probes demonstrated that this library has no organellar contamination. The 5460S library was screened with 3 molecular markers linked to tmsl gene as probes and at least 1 BAC clone was identified with each probe. The insert ends of positive clones were successfully isolated using thermal asymmetric interlaced PCR (TAIL-PCR) technique.

  4. Widespread distribution of the piggyBac transposon in various bactrocera species

    International Nuclear Information System (INIS)

    Full text: The piggyBac transposable element from the Lepidopteran species Trichoplusia ni is currently the most widely used vector for insect transgenesis. Consequently, the presence of piggyBac-like sequences has been investigated, by PCR and Southern analysis, in different species of target genera such as Ceratitis, Bactrocera and Anastrepha, along with Tirhitromina and Rhagoletis. PiggyBac-like sequences were detected in several Bactrocera species. The evolution of the piggyBac-like sequences is discussed with respect to the phylogenies of the hosts. (author)

  5. DLESE Teaching Box Pilot Project: Developing a Replicable Model for Collaboratively Creating Innovative Instructional Sequences Using Exemplary Resources in the Digital Library for Earth System Education (DLESE)

    Science.gov (United States)

    Weingroff, M.

    2004-12-01

    Before the advent of digital libraries, it was difficult for teachers to find suitable high-quality resources to use in their teaching. Digital libraries such as DLESE have eased the task by making high quality resources more easily accessible and providing search mechanisms that allow teachers to 'fine tune' the criteria over which they search. Searches tend to return lists of resources with some contextualizing information. However, teachers who are teaching 'out of discipline' or who have minimal training in science often need additional support to know how to use and sequence them. The Teaching Box Pilot Project was developed to address these concerns, bringing together educators, scientists, and instructional designers in a partnership to build an online framework to fully support innovative units of instruction about the Earth system. Each box integrates DLESE resources and activities, teaching tips, standards, concepts, teaching outcomes, reviews, and assessment information. Online templates and best practice guidelines are being developed that will enable teachers to create their own boxes or customize existing ones. Two boxes have been developed so far, one on weather for high school students, and one on the evidence for plate tectonics for middle schoolers. The project has met with significant enthusiasm and interest, and we hope to expand it by involving individual teachers, school systems, pre-service programs, and universities in the development and use of teaching boxes. A key ingredient in the project's success has been the close collaboration between the partners, each of whom has brought unique experiences, perspectives, knowledge, and skills to the project. This first effort involved teachers in the San Francisco Bay area, the University of California Museum of Paleontology, San Francisco State University, U.S. Geological Survey, and DLESE. This poster will allow participants to explore one of the teaching boxes. We will discuss how the boxes were

  6. Services and Resources to Engineers: A Case Study of Outreach and Marketing, Assessment, and Future Directions in a Research Library

    Science.gov (United States)

    Torrence, Matt

    2015-01-01

    The literature, activities, and resource needs of engineering students and faculty provide insight into a demographic that is often among the early-adopters of new technologies, tools, and methods of sharing information. Despite the often non-bibliographic nature of their research efforts, there are numerous elements of the traditional service…

  7. Sorghum Mutant Library as a Genetic Resource to Improve Biomass Yield and BioEnergy Conversion Efficiency

    Science.gov (United States)

    Due to the dwindling of mineral energy resources and soaring fuel price, agriculture sector is called to provide bioenergy in addition to the traditional role of providing humanity with food, fiber, and feed. Freshwater is likely a major challenge for developing biofuels because the trend of global ...

  8. Evidence Based Research Activities, Interests and Opportunities Exist for Practitioners in all Library Sectors in the British Isles. A review of: McNicol, Sarah. “Is Research an Untapped Resource in the Library and Information Profession?” Journal of Librarianship and Information Science 36.3 (September 2004:119-26.

    Directory of Open Access Journals (Sweden)

    Julie McKenna

    2006-03-01

    technology (ICT was an area of planned future research in all libraries, as were user needs and user behaviour. The most frequently cited barriers to research activity across all sectors were lack of time and financial resources. Staff skills and the lack of focus on practical problems to solve were indicated as a barrier in health, public and academic libraries. Libraries reported a range of common uses for the research findings including: informing strategic and service planning; providing benchmarking data and measuring the effectiveness of services; identification of marketing and public relations opportunities; discovery of staff training needs; and use of the results to demonstrate the value of libraries to funding bodies. Conclusion – This study provides insight into practitioner‐focused areas of research interest and possible areas for future investigation. As the author reports in her conclusion, the survey results cannot be considered representative of the wider population. Since research interests often overlap, a sector wide or cross‐sectoral research approach should be considered to allow library staff to identify and resolve common problems. Wide dissemination of research results within the practitioner community would be of benefit to all. Greater communication between practitioner and information science communities is also encouraged, as these communities’ work is mutually beneficial.

  9. The Construction of Library Digital Resources under the Background of the Development of Public Digital Culture%公共数字文化发展背景下的图书馆数字资源建设

    Institute of Scientific and Technical Information of China (English)

    许建业

    2015-01-01

    论文概述了公共数字文化发展背景,探讨了图书馆在公共数字文化建设中的定位与任务,以江苏全省公共图书馆数字化建设和南京图书馆数字资源建设实践为例,围绕图书馆数字资源建设主要内容,提出了相关建议和措施。%This paper outlines the background of public digital culture development, and discusses the positioning and task of library in the construction of public digital culture. Taking the digital construction of public libraries in Jiangsu Province and the concrete practice of Nanjing Library as examples, it puts forward the relevant suggestions and measures on library digital resources construction.

  10. On Library Work Reorganization Based on the Information Resource Integration%基于信息资源整合的图书馆工作重组探讨

    Institute of Scientific and Technical Information of China (English)

    王蓉

    2015-01-01

    本文论述了高校图书馆信息资源整合的现状及整合中存在的问题,并阐明了信息资源整合的意义,积极探讨信息资源整合过程中图书馆的部门设置、人力资源以及业务流程的重组问题。%This paper discusses the present situation of university library's information resources integration and the existing problems in the integration, and illustrates the significance of information resource integration, actively explores the library department settings, human resource and business process restructuring in information resources integration.

  11. LMOD:一种基于本体描述的电子学习资源库模型%LMOD:e-learning resource library model based on ontology describing

    Institute of Scientific and Technical Information of China (English)

    张沪寅; 李鑫; 陆春涛; 吴笛

    2011-01-01

    为了解决现有资源库在资源检索查全率、查准率上存在的不足,将语义特征引入到电子学习资源库模型的构建之中,提出了一种基于本体描述的电子学习资源库模型LMOD.LMOD通过构建电子学习资源库本体并生成相应的语义元数据,规范地描述资源,将资源以机器可理解的方式组织起来;并利用语义学视图规范用户查询语句.在此基础上实现基于语义的资源查询,以有效地解决搜索中含糊解释与同义识别的问题.实验证明,该模型能够有效克服现有资源库建设方案的缺点,提升资源库的可用性与使用效率.%In order to solve the defect of existing resource library on recall and precision of resource retrieval, this article introduced the semantic characteristic into the construction of e-lear-ning resource library model, proposed an e-lear-ning resource library model based on ontology describing which named LMOD. LMOD described the resources normative by building e-learning resource library ontology and generating correlative semantic metadata, organized the resources in machine-understandable way, and standardized user query by utilizing the semantics view. On this basis, LMOD achieved the semantic-based query in order to effectively address the problems such as ambiguous interpretation and synonym recognition in resource retrieval. The experiment proves that the model can overcome the defect of existing resource library building scheme and enhance the service efficiency and availability of e-learning resource library.

  12. Mobile technologies for every library

    CERN Document Server

    Gleason, Ann Whitney

    2015-01-01

    If you are wondering what mobile technology adoption means for your library or how to get started, Mobile Technologies for Every Library will answer your questions! This book will detail the opportunities and pitfalls in using mobile technology in libraries. It will also address platforms, options, security, and best practices for starting new mobile services programs or improving existing services. Gleason previews many useful apps for libraries. Web links and resources are also included.

  13. Standards for hospital libraries 2002

    OpenAIRE

    Gluck, Jeannine Cyr; Hassig, Robin Ackley; Balogh, Leeni; Bandy, Margaret; Doyle, Jacqueline Donaldson; Kronenfeld, Michael R.; Lindner, Katherine Lois; Murray, Kathleen; Petersen, JoAn; Rand, Debra C.

    2002-01-01

    The Medical Library Association's “Standards for Hospital Libraries 2002” have been developed as a guide for hospital administrators, librarians, and accrediting bodies to ensure that hospitals have the resources and services to effectively meet their needs for knowledge-based information. Specific requirements for knowledge-based information include that the library be a separate department with its own budget. Knowledge-based information in the library should be directed by a qualified libr...

  14. Strategic marketing planning in library

    OpenAIRE

    Karmen Štular-Sotošek

    2000-01-01

    The article is based on the idea that every library can design instruments for creating events and managing the important resources of today's world, especially to manage the changes. This process can only be successful if libraries use adequate marketing methods. Strategic marketing planning starts with the analysis of library's mission, its objectives, goals and corporate culture. By analysing the public environment, the competitive environment and the macro environment, libraries recognise...

  15. Investigation into the Utilization Situation of Learning Resources of Digital Libraries%数字图书馆学习资源使用现状调查分析

    Institute of Scientific and Technical Information of China (English)

    柏菊花; 陶秋荣; 于素云; 徐明

    2011-01-01

    Investigation into the utilization situation of learning resources of digital libraries by master of educational technology in about 20 universities shows that there is no logic relationship between learning resources of digital libraries and utilization average; there are complicated hindering factor in the utilization of learning resources of digital libraries; the utilization rate of digital libraries is lower than the utilization rate of search engine; users are lack of awareness of resources contribution. Therefore, we should research the utilization situation of learning resources by users continually, in order to improve library services and help readers to use learning resources better.%通过对我国近20所高校教育技术学专业的硕士研究生利用数字图书馆学习资源的调查表明,数字图书馆学习资源的评价和使用均值之间没有必然联系影响用户使用数字图书馆学习资源的阻碍因素复杂;数字图书馆的使用率低于搜索引擎;用户缺乏资源贡献意识。因此,应继续研究用户利用学习资源的情况,从而改善自身服务,帮助读者更好地利用学习资源。

  16. 以科学发展观加强民办高校图书馆人力资源建设%Strengthening Human Resources Construction of Private College Libraries with the Scientific Concept of Development

    Institute of Scientific and Technical Information of China (English)

    杨林超

    2012-01-01

    图书馆是高校进行文化熏陶的重要场所,因此,高校需要更加重视图书馆人力资源建设,尤其是民办高校.本文以科学发展现为角度来探讨如何加强民办高校图书馆人力资源建设,这也是是新时期下图书馆发展的必然途径.%The library- is an important place of culture in colleges and universities, therefore, colleges and universities need to pay more attention to human resources construction of libraries, especially in private institutions. This article discusses how to strengthen the human resources construction of private institutions from the scientific development concept which is also the inevitable trend of library development in the new period.

  17. 图书馆光盘型电子资源编目方法探析%Aanlysis of the Cataloging Method for CD-ROM Resources in Library

    Institute of Scientific and Technical Information of China (English)

    王晶

    2011-01-01

    This paper defines the conception of CD-ROM resources,and analyses the cataloging for CD-ROM resources in library with the examples of cataloging work.%对光盘型电子资源进行了界定,结合工作实例对图书馆光盘型电子资源的编目进行分析。

  18. Construction of a full bacterial artificial chromosome (BAC) library of Oryza sativa genome

    Institute of Scientific and Technical Information of China (English)

    TAOQUANZHOU; HAIYINGZHAO; 等

    1994-01-01

    We have constructed a full BAC library for the superior early indica variety of Oryza sativa,Guang Lu Ai 4.The MAX Efficiency DH10B with increased stability of inserts was used as BAC host cells.The potent pBelo BACII with double selection markers was used as cloning vector.The cloning efficiency we have reached was as high as 98%,and the transformation efficiency was raised up to 106 transformants/μg of large fragment DNA.The BAC recombinant transformants were picked at random and analyzed for the size of inserts,which turned out to be of 120 kb in length on average.We have obtained more than 20,000 such BAC clones.According to conventional probability equation,they covered the entire rice genome of 420,000 kb in length.The entire length of inserts of the library obtained has the 5-to 6-fold coverage of the genome.To our knowledge,this is the first reported full BAC library for a complex genome.

  19. Cloud Computing and Your Library

    Science.gov (United States)

    Mitchell, Erik T.

    2010-01-01

    One of the first big shifts in how libraries manage resources was the move from print-journal purchasing models to database-subscription and electronic-journal purchasing models. Libraries found that this transition helped them scale their resources and provide better service just by thinking a bit differently about their services. Likewise,…

  20. 区域性高校图书馆信息资源共建共享研究%A Study of the Co-construction and Sharing of Information Resources in Regional Universities Libraries

    Institute of Scientific and Technical Information of China (English)

    谢杰民

    2012-01-01

    Because of geographical proximity,regional university libraries have more unique advantages to realize the co-construction and sharing of information resources.This paper elaborates the necessity of the co-construction and sharing of information resources in regional universities libraries,analyses the types and characteristics of library information resources co-construction and sharing currently,and puts forward the construction and implementation measures of information resources co-construction and sharing system in regional universities libraries under network environment.%区域性高校图书馆由于地理位置邻近,实现信息资源共建共享更具有独特的优势。在阐述区域性高校图书馆信息资源共建共享必要性的基础上,分析目前我国区域性高校图书馆信息资源共建共享类型与特点,提出网络环境下区域性高校图书馆信息资源共建共享体系的构建及实施措施。

  1. 高校图书馆开展地方文献资源建设工作探析%Discussion on construction of local document resources in university libraries

    Institute of Scientific and Technical Information of China (English)

    朱晓

    2013-01-01

    地方文献是一种独特的文献资源,具有地域性、多样性,是高校图书馆服务社会发展、推进文化传承创新的重要文献保障。切实做好地方文献资源建设工作,可以提升高校图书馆的核心服务能力,实现高校图书馆可持续发展。%Local documents as a kind of unique document resources occupy a special position in the collection sys -tem in university libraries , which provide resource support to develop characteristic service and improve ability of information service .The regional characteristics and diversities of local document provide important document secu -rity through which university libraries serve the economic and social development and promote the cultural inherit -ance and innovation .University libraries'effective construction of document resources can promote their core service ability based on local characteristics of the document information resources , which will assist university libraries in sustainable development in the face of the challenges of informatization , automation, digitization, diversification in today's society .

  2. 关于提高公安院校图书馆数字资源利用率的探析%On the Improvement of Digital Resource Utilization Ratio of Police Universities' Libraries

    Institute of Scientific and Technical Information of China (English)

    信晓红

    2012-01-01

    Police universities need to develop digital resource in library, but the existing conditions make the construction of library digital resource difficult to develop to an extent. So libraries are short of unique resources and difficult to attract the existing reader groups, the utilization ratio is low. We should provide the service of developing readers, build digital resource database and offer digital resource service, so we can improve digital resource utilization ratio.%公安院校图书馆需要发展数字资源馆藏,但现有条件使得数字资源建设难以形成规模,造成馆藏中现有的数字资源缺乏独到的资源优势,难以吸引现有的用户群体,利用率较低,需要从读者培养服务、数字馆藏建设、数字资源服务手段等方面加以调整,以提高馆藏数字资源的利用率。

  3. Probe into the Information Resources Construction of the Libraries of Private Colleges and Universities---Taking the Library of Guangdong Peizheng College as an Example%民办本科院校图书馆信息资源建设浅探--以广东培正学院图书馆为例

    Institute of Scientific and Technical Information of China (English)

    冯敏英

    2014-01-01

    Taking the library of Guangdong Peizheng College as an example,this paper analyzes the current status of and problems existing in the information resources construction in the libraries of private colleges and universities ,and puts forward some countermeasures for carrying out the information resources construction in the libraries of private colleges and universities.%以广东培正学院图书馆为例,分析了民办本科院校图书馆信息资源建设的现状和存在的问题,探讨了民办本科院校信息资源建设的对策。

  4. Library Instruction in the Electronic Library: The University of Arizona's Electronic Library Education Centers.

    Science.gov (United States)

    Glogoff, Stuart

    1995-01-01

    Discusses two Electronic Library Education Centers (ELECs) created at the University of Arizona to improve library instruction in the use of online resources. Examines costs of developing ELECs; technical changes experienced; and benefits to users and librarians. A sidebar by Abbie J. Basile identifies Internet resources for planning and/or…

  5. Genomic Resources for Water Yam (Dioscorea alata L.: Analyses of EST-Sequences, De Novo Sequencing and GBS Libraries.

    Directory of Open Access Journals (Sweden)

    Christopher A Saski

    Full Text Available The reducing cost and rapid progress in next-generation sequencing techniques coupled with high performance computational approaches have resulted in large-scale discovery of advanced genomic resources in several model and non-model plant species. Yam (Dioscorea spp. is a major food and cash crop in many countries but research efforts have been limited to understand the genetics and generate genomic information for the crop. The availability of a large number of genomic resources including genome-wide molecular markers will accelerate the breeding efforts and application of genomic selection in yams. In the present study, several methods including expressed sequence tags (EST-sequencing, de novo sequencing, and genotyping-by-sequencing (GBS profiles on two yam (Dioscorea alata L. genotypes (TDa 95/00328 and TDa 95-310 was performed to generate genomic resources for use in its improvement programs. This includes a comprehensive set of EST-SSRs, genomic SSRs, whole genome SNPs, and reduced representation SNPs. A total of 1,152 EST-SSRs were developed from >40,000 EST-sequences generated from the two genotypes. A set of 388 EST-SSRs were validated as polymorphic showing a polymorphism rate of 34% when tested on two diverse parents targeted for anthracnose disease. In addition, approximately 40X de novo whole genome sequence coverage was generated for each of the two genotypes, and a total of 18,584 and 15,952 genomic SSRs were identified for TDa 95/00328 and TDa 95-310, respectively. A custom made pipeline resulted in the selection of 573 genomic SSRs common across the two genotypes, of which only eight failed, 478 being polymorphic and 62 monomorphic indicating a polymorphic rate of 83.5%. Additionally, 288,505 high quality SNPs were also identified between these two genotypes. Genotyping by sequencing reads on these two genotypes also revealed 36,790 overlapping SNP positions that are distributed throughout the genome. Our efforts in using

  6. International librarianship. An electronic resources pathfinder. A term paper for the References Services course at the Master in Library Science Program, Department of Library and Information Studies, State University of New York at Buffalo, Buffalo, NY (USA)

    OpenAIRE

    Muela-Meza, Zapopan Martín

    2001-01-01

    This library guide or pathfinder is made as a term paper for the References Services course at the Master in Library Science Program at the State University of New York at Buffalo, Buffalo, NY, USA; Associate Professor of the course Dr. Lorna Peterson, April 23, 2001. It lists sources in electronic format to introduce undergraduate and graduate students of the State University of New York at Buffalo to the research and study of Library and Information Science with an international scope. Cons...

  7. Books, children, dogs, artists: library programs for the entire family.

    Science.gov (United States)

    Haver, Mary Katherine

    2014-01-01

    The promotion of library resources and services is a continuous process for all libraries, especially hospital family resource center libraries. Like public libraries, a family resource center can utilize programs as a pathway for connecting with and developing awareness of library resources and services available to patient families. This column describes the programs currently offered for All Children's Hospital Johns Hopkins Medicine patient families, marketing initiatives to promote these programs, and utilization of grant funding to supplement a program.

  8. Books, children, dogs, artists: library programs for the entire family.

    Science.gov (United States)

    Haver, Mary Katherine

    2014-01-01

    The promotion of library resources and services is a continuous process for all libraries, especially hospital family resource center libraries. Like public libraries, a family resource center can utilize programs as a pathway for connecting with and developing awareness of library resources and services available to patient families. This column describes the programs currently offered for All Children's Hospital Johns Hopkins Medicine patient families, marketing initiatives to promote these programs, and utilization of grant funding to supplement a program. PMID:24735270

  9. On the Construction of Subject Resources and Subject Service in American Academic Libraries:Taking Four Business Libraries in America as Examples%美国高校图书馆学科资源建设及学科服务研究——以四所美国商学院图书馆为例

    Institute of Scientific and Technical Information of China (English)

    蔡金燕

    2015-01-01

    American academic libraries are experienced in the construction of subject resources and subject service, especially in the business libraries. Various categories of resources, like physical resources, databases, kinds of guides, course resources, self-built databases, are abundant in quantity in these business libraries. They provide subject services focusing on courses, researches and careers. Chinese academic libraries should learn from them.%美国高校图书馆学科资源建设和学科服务实践成熟、经验丰富,尤其是商学院图书馆,实体资源、电子数据库、各种资源导航、课程资源、自建数据库等资源丰富,实现以课程为中心、以研究为中心、以职业为中心三种方式进行学科服务,值得国内高校图书馆借鉴.

  10. A non-autonomous insect piggyBac trasposable element is mobile in tobacco

    Science.gov (United States)

    The piggyBac transposable element, originally isolated from a virus in an insect cell line, is a valuable molecular tool for transgenesis and mutagenesis of invertebrates. For heterologous transgenesis in a variety of mammals, transfer of the piggyBac transposable element from an ectopic plasmid onl...

  11. Protective efficacy of a recombinant BAC clone of Marek's disease virus containing REV-LTR

    Science.gov (United States)

    Insertion of reticuloendotheliosis virus (REV) long-terminal repeat (LTR) into a bacterial artificial chromosome (BAC) clone of a very virulent strain of Marek’s disease (MD) virus (MDV), Md5 (Kim et al, 2011) rendered the resultant recombinant virus termed rMd5 REV-LTR BAC fully attenuated at passa...

  12. Recombineering strategies for developing next generation BAC transgenic tools for optogenetics and beyond

    Directory of Open Access Journals (Sweden)

    Jonathan T Ting

    2014-04-01

    Full Text Available The development and application of diverse BAC transgenic rodent lines has enabled rapid progress for precise molecular targeting of genetically-defined cell types in the mammalian central nervous system. These transgenic tools have played a central role in the optogenetic revolution in neuroscience. Indeed, an overwhelming proportion of studies in this field have made use of BAC transgenic cre driver lines to achieve targeted expression of optogenetic probes in the brain. In addition, several BAC transgenic mouse lines have been established for direct cell-type specific expression of Channelrhodopsin-2 (ChR2. While the benefits of these new tools largely outweigh any accompanying challenges, many available BAC transgenic lines may suffer from confounds due in part to increased gene dosage of one or more ‘extra’ genes contained within the large BAC DNA sequences. Here we discuss this under-appreciated issue and propose strategies for developing the next generation of BAC transgenic lines that are devoid of extra genes. Furthermore, we provide evidence that these strategies are simple, reproducible, and do not disrupt the intended cell-type specific transgene expression patterns for several distinct BAC clones. These strategies may be widely implemented for improved BAC transgenesis across diverse disciplines.

  13. Putting the Library at Students' Fingertips

    Science.gov (United States)

    Foley, Marianne

    2012-01-01

    The absence of a well-defined space for library resources within course-management systems has been well documented in library literature. Academic libraries have sought to remedy this deficiency in numerous ways. This article describes how a "library nugget," or module, was added to the Buffalo State College course-management system, ANGEL. The…

  14. Library Employment Sources on the Internet

    Science.gov (United States)

    Barr, Catherine

    2012-01-01

    This article presents a list of online resources for library job seekers. This includes general sites/portals like American Library Association (ALA): Education & Careers and Canadian Library Association: Library Careers. It also includes sites by sector, employment agencies/commercial services, listservs and networking sites.

  15. Illinois: Library Automation and Connectivity Initiatives.

    Science.gov (United States)

    Lamont, Bridget L.; Bloomberg, Kathleen L.

    1996-01-01

    Discussion of library automation in Illinois focuses on ILLINET, the Illinois Library and Information Network. Topics include automated resource sharing; ILLINET's online catalog; regional library system automation; community networking and public library technology development; telecommunications initiatives; electronic access to state government…

  16. A Library Media Guide for Vermont Schools.

    Science.gov (United States)

    Vermont State Dept. of Education, Montpelier. Div. of Federal Assistance.

    This guide is designed to be used in developing and improving school library media programs and in teaching students how to use the library. Topics covered include: general philosophy; specific goals; roles and responsibilities; resources needed; creating a library media center; curriculum (including a brief scope and sequence of library skills);…

  17. Cloning and expression of human UDP-glucuronosyltransferase 1A4 in Bac-to-Bac system

    International Nuclear Information System (INIS)

    UDP-glucuronosyltransferases (UGTs) catalyze the transfer of glucuronic acid from uridine diphosphate-glucuronic acid (UDP-GA) to compounds with amine, hydroxyl, and carboxylic acid moieties. N-glucuronidation is an important pathway for elimination of many tertiary amine therapeutic agents used in humans. UGT1A4 has been reported to be specific for glucuronidating primary, secondary, and tertiary amines, forming N-glucuronides. To further investigate the drugs metabolized by UGT1A4, the Bac-to-Bac expression system was used to express the recombinant UGT1A4 with His-tag on the C-terminal. The His-tagged recombinant UGT1A4 expressed in Spodoptera frugiperda (Sf9) cells were detected using anti-His antibody and the molecular weight of the recombinant protein was approximately 55 kDa. The enzyme activity towards imipramine in cell homogenate protein was found to be 83.14 ± 15 pmol/min/mg protein (n=3) with 0.5 mM imipramine by HPLC, but was not detectable in blank Sf9 cells. It paved the way for the further studies for drug glucuronidation by UGT1A4. The purification of the UGT1A4 can be done by Ni-resin. This is helpful to do research on the structure of the UFT1A4

  18. 依托文化资源共享工程拓展县图书馆延伸服务%Relying on Cultural Resources Sharing Project, Developing the County Library Extension Services

    Institute of Scientific and Technical Information of China (English)

    曹志萍

    2012-01-01

    This paper analyzes the coexistence reality of opportunities and challenges of the county libraries under the network environment, proposes the county library must change the service concept, improve the construction of the library. By relying on the excellent resources of the National Cultural Resources Sharing Project, county library will expand the county level library extension services through service to the county ,community and rural areas so that the cultural information will spread to the county wide and provide full service to people of the county.%本文分析了网络环境下县级图书馆机遇与挑战并存的现实,提出县级图书馆必须转变服务理念,搞好图书馆阵地建设,并依托全国文化资源共享工程优秀资源,拓展县级图书馆延伸服务,通过服务县城、服务社区、服务农村,使文化信息真正普及到全县范围,全方位服务全县人民。

  19. 国内主要医药图书馆数字资源建设现状调查%Status quo of digital resources development in domestic medical and pharmaceutical libraries

    Institute of Scientific and Technical Information of China (English)

    任慧玲; 汪庆; 周琴

    2015-01-01

    选取中国高等院校医药图书馆协会8所图书馆作为调研对象,统计各图书馆数字资源的语种、类型及数量,分析发现上述图书馆数字资源建设已经取得一定成效,但是存在新型数字资源建设有待加强、免费及开放资源整合不够、资源组织揭示不够深入等问题,并对此提出了优化数字资源结构、建设特色数字资源、深入组织揭示数字资源等对策。%The languages, types and number of digital resources in 8 domestic medical and pharmaceutical college and university libraries were analyzed , which showed that although certain successes have been achieved in devel-opment of digital resources in these libraries, there are some problems needing to be solved, such as weakness in new digital resources development , insufficient integration of free and open access resources , insufficient organiza-tion and revealing of resources .Suggestions were put forward for the solution of these problems , such as optimizing digital resource structure, developing characteristic digital resources, sufficient organizing and revealing digital re-sources.

  20. Analysis and location of a rice BAC clone containing telomeric DNA sequences

    Institute of Scientific and Technical Information of China (English)

    翟文学; 陈浩; 颜辉煌; 严长杰; 王国梁; 朱立煌

    1999-01-01

    BAC2, a rice BAC clone containing (TTTAGGG)n homologous sequences, was analyzed by Southern hybridization and DNA sequencing of its subclones. It was disclosed that there were many tandem repeated satellite DNA sequences, called TA352, as well as simple tandem repeats consisting of TTTAGGG or its variant within the BAC2 insert. A 0. 8 kb (TTTAGGG) n-containing fragment in BAC2 was mapped in the telomere regions of at least 5 pairs of rice chromosomes by using fluorescence in situ hybridization (FISH). By RFLP analysis of low copy sequences the BAC2 clone was localized in one terminal region of chromosome 6. All the results strongly suggest that the telomeric DNA sequences of rice are TTTAGGG or its variant, and the linked satellite DNA TA352 sequences belong to telomere-associated sequences.

  1. Back to BAC: The Use of Infectious Clone Technologies for Viral Mutagenesis

    Directory of Open Access Journals (Sweden)

    Robyn N. Hall

    2012-02-01

    Full Text Available Bacterial artificial chromosome (BAC vectors were first developed to facilitate the propagation and manipulation of large DNA fragments in molecular biology studies for uses such as genome sequencing projects and genetic disease models. To facilitate these studies, methodologies have been developed to introduce specific mutations that can be directly applied to the mutagenesis of infectious clones (icBAC using BAC technologies. This has resulted in rapid identification of gene function and expression at unprecedented rates. Here we review the major developments in BAC mutagenesis in vitro. This review summarises the technologies used to construct and introduce mutations into herpesvirus icBAC. It also explores developing technologies likely to provide the next leap in understanding these important viruses.

  2. Technological exploration of BAC-FISH on mitotic chromosomes of maize

    Institute of Scientific and Technical Information of China (English)

    Yongsheng TAO; Zuxin ZHANG; Yonglin CHEN; Lijia LI; Yonglian ZHENG

    2008-01-01

    The rice BAC-DNA was used as probes and fluorescence in situ hybridization (FISH) was applied to the interphase and metaphase mitotic chromosomes of maize. To optimize the BAC-FISH technique, we respect-ively assayed the effect of several factors, including maize or rice genomic Cot DNA used as blocking reagent of DNA, washing temperatures and FAD concentration in the washing buffer and in the hybrid solution. The results show that Cot DNA of maize genome blocked the repet-itive sequence of the rice BAC-DNA when the Cot value was below 50. Meanwhile, it was necessary to adjust the Cot value according to the different probes and their ratios. Decreasing the concentration of FAD in the hybridization mixtures, adjusting the washing rate after hybridization, and most especially, blocking the rice-specific repetitive sequences of BAC-DNA could improve the positive signals of BAC-FISH.

  3. A Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC) Transgenesis in Zebrafish.

    Science.gov (United States)

    Fuentes, Fernando; Reynolds, Eric; Lewellis, Stephen W; Venkiteswaran, Gayatri; Knaut, Holger

    2016-01-01

    Transgenesis of large DNA constructs is essential for gene function analysis. Recently, Tol2 transposase-mediated transgenesis has emerged as a powerful tool to insert bacterial artificial chromosome (BAC) DNA constructs into the genome of zebrafish. For efficient transgenesis, the genomic DNA piece in the BAC construct needs to be flanked by Tol2 transposon sites, and the constructs should contain a transgenesis marker for easy identification of transgenic animals. We report a set of plasmids that contain targeting cassettes that allow the insertion of Tol2 sites and different transgenesis markers into BACs. Using BACs containing these targeting cassettes, we show that transgenesis is as efficient as iTol2, that preselecting for expression of the transgenesis marker increases the transgenesis rate, and that BAC transgenics faithfully recapitulate the endogenous gene expression patterns and allow for the estimation of the endogenous gene expression levels. PMID:26818072

  4. PR: Priority for Community College Libraries.

    Science.gov (United States)

    Vaughan, Elinor F.

    1987-01-01

    Describes the Stanly Technical College Library's public relations program which attempts to encourage the use of library and media resources and to promote the college as a major community resource. Highlights library activities, including a photography contest for students and staff, and displays featuring college programs and local history. (DMM)

  5. Parent Involvement in Elementary School Libraries

    Science.gov (United States)

    Copeland, Michele Rzewski

    2012-01-01

    In the 21st century, school libraries are under pressure to innovate. Library budgets are frequently slashed as districts struggle with limited fiscal resources, while library personnel are increasingly expected to provide students with resources they need to help them pass high stakes tests. In an effort to meet student needs with limited…

  6. 信息异化对图书馆信息资源建设的影响%The Impact of Information Alienation on the Construction of Library Information Resources

    Institute of Scientific and Technical Information of China (English)

    邵胜春

    2014-01-01

    This article discusses the influence of information alienation for the construction of library information re-sources from the following aspects including the user ’ s perception of library information resource construction ,sub-ject consciousness ,information integration service .The article expounds strategy of the control information alienation phenomenon in information resource construction from the following aspects including strengthening the training of library human consciousness ,to provide effective information resources for users based on user demand ,to carry out information resource integration service based on user demand .%从用户对图书馆信息资源建设的认知、主体人意识的培养、信息集成服务等方面探讨了信息异化对图书馆信息资源建设的影响,并从加强图书馆人主体意识的培养;以需求为导向,为用户提供有效信息资源;基于用户需求,开展信息资源集成服务等方面,阐述了图书馆在信息资源建设中控制信息异化现象的策略。

  7. The Faced Challenge and Innovation Development of Military Academy Library in Resources Co-construction & Sharing%军校图书馆资源共建共享面临的挑战与创新发展

    Institute of Scientific and Technical Information of China (English)

    于厚海; 赵正国

    2015-01-01

    新时期军校图书馆资源共建共享面临着严峻挑战和发展机遇。当前制约图书馆资源共建共享的主要因素包括主观和客观两个方面。必须从理念、机制和制度等方面入手,推进军校图书馆资源共建共享体系创新发展。%The resources co -construction and sharing of military academy library face new challenges and opportunities in the new era. Nowadays, the factors restraining the resources co-construction and sharing include in both subjective and objective aspects. It is necessary to promote the innovative development system for resources co-construction and sharing of military academy library from the aspects of concept, mechanism, and institution, etc.

  8. Research on Information Resources Promotion and Service Work of University Library%高校图书馆信息资源推广与服务工作探究

    Institute of Scientific and Technical Information of China (English)

    王玘珺

    2016-01-01

    The age of the Internet for the development of the university library has brought challenges and opportunities. The Internet is convenient and quick the students access to information resources and network applications into the preferred way. The library as the traditional information resources reserves and to provide information on site, its nature decides the traditional library low-key introverted style, the era of today, the proliferation of Internet application libraries are beginning to more and more by the attention of the students, in the face of adversity, the university library should take the initiative to go out, make full use of the advantages of the new media applied to library information resources to promote the work to.%互联网的时代为高校图书馆的发展带来了挑战也带来了机遇。互联网的方便快捷使得大学生在获取信息资源的途径上,网络应用成了首选途径。图书馆作为传统信息资源储备和提供信息的重要场所,其性质决定着传统图书馆低调内敛的格调,如今互联网应用泛滥的时代下,图书馆开始越来越不受到大学生们的重视,面对窘境,高校图书馆应该主动走出去,充分利用新媒介的优势运用到图书馆的信息资源推广工作中去。

  9. 东莞图书馆数字资源的整合建设与便捷服务%The Integrated Construction and Convenient Services of Digital Resources in Dongguan Library

    Institute of Scientific and Technical Information of China (English)

    杨累

    2012-01-01

    信息社会和数字出版不断发展,数字阅读日益流行,数字资源成为公共图书馆的重要资源,如何更好地建设数字资源并开展服务成为公共图书馆面临的新课题.文章论述了东莞图书馆数字资源整合建设的内容、原则和具体措施,随之结合东莞图书馆的实践分析了一些具体的便捷服务举措,并提出了几点问题及思考.%With the continuous development of information society and digital publishing, digital reading has become increasingly popular, and digital resources have become an important resource for public libraries. As a result, how to better conduct its construction and service has become a new topic which public libraries are faced with. The article discusses the content, principle and specific initiatives of digital resources integration in Dongguan library, analyses a few specific convenient initiatives along with the practice of Dongguan library, and proposed several issues and thoughts.

  10. Library Automation

    OpenAIRE

    Dhakne, B. N.; Giri, V. V; Waghmode, S. S.

    2010-01-01

    New technologies library provides several new materials, media and mode of storing and communicating the information. Library Automation reduces the drudgery of repeated manual efforts in library routine. By use of library automation collection, Storage, Administration, Processing, Preservation and communication etc.

  11. Methylation-sensitive linking libraries enhance gene-enriched sequencing of complex genomes and map DNA methylation domains

    Directory of Open Access Journals (Sweden)

    Bharti Arvind K

    2008-12-01

    Full Text Available Abstract Background Many plant genomes are resistant to whole-genome assembly due to an abundance of repetitive sequence, leading to the development of gene-rich sequencing techniques. Two such techniques are hypomethylated partial restriction (HMPR and methylation spanning linker libraries (MSLL. These libraries differ from other gene-rich datasets in having larger insert sizes, and the MSLL clones are designed to provide reads localized to "epigenetic boundaries" where methylation begins or ends. Results A large-scale study in maize generated 40,299 HMPR sequences and 80,723 MSLL sequences, including MSLL clones exceeding 100 kb. The paired end reads of MSLL and HMPR clones were shown to be effective in linking existing gene-rich sequences into scaffolds. In addition, it was shown that the MSLL clones can be used for anchoring these scaffolds to a BAC-based physical map. The MSLL end reads effectively identified epigenetic boundaries, as indicated by their preferential alignment to regions upstream and downstream from annotated genes. The ability to precisely map long stretches of fully methylated DNA sequence is a unique outcome of MSLL analysis, and was also shown to provide evidence for errors in gene identification. MSLL clones were observed to be significantly more repeat-rich in their interiors than in their end reads, confirming the correlation between methylation and retroelement content. Both MSLL and HMPR reads were found to be substantially gene-enriched, with the SalI MSLL libraries being the most highly enriched (31% align to an EST contig, while the HMPR clones exhibited exceptional depletion of repetitive DNA (to ~11%. These two techniques were compared with other gene-enrichment methods, and shown to be complementary. Conclusion MSLL technology provides an unparalleled approach for mapping the epigenetic status of repetitive blocks and for identifying sequences mis-identified as genes. Although the types and natures of

  12. 外语院校图书馆数字资源的建设现状与发展对策%Construction Status Quo and Development Strategies of Digital Resources in Libraries of Foreign Studies Universities

    Institute of Scientific and Technical Information of China (English)

    佘军; 林玲

    2012-01-01

    通过对国内8所外语院校图书馆的调查可知,目前我国外语院校图书馆数字资源建设存在数据库重复建设严重、自建特色数据库少且质量不高、网络资源开发不够等问题。因此,外语院校图书馆应通过全国外语院校图书馆联盟进行整体规划,协调采购数字资源,联合建设特色数据库,整合各类数字资源,实现外语院校图书馆数字资源的优化配置,发挥数字资源的最大效益,为外语院校的教学和科研服务。%Through the investigation into 8 libraries of foreign studies universities, we find out that currently there are some problems in the construction of digital resources, such as the repeated construction of databases, low quality of self-built characteristic databases, the insufficient development of network resources, and so on. Therefore, it is necessary for libraries of foreign studies universities to carry out overall planning, purchase digital resources collaboratively, construct characteristic databases jointly, integrate various digital resources, realize the optimized allocation of digital resources in libraries of foreign studies universities, give full play of the greatest benefit of digital resources, and provide services for teaching and researches in foreign studies universities, through the national library consortium of foreign studies universities.

  13. On the Functions of Vocational School Libraries and the Optimization of Resource Allocation%论职业学校图书馆职能与优化资源配置

    Institute of Scientific and Technical Information of China (English)

    王惠卿

    2013-01-01

    Starting from the library's role of book collection, infor-mation development and education, this paper explores the ap-proaches to optimize the resource allocation of libraries.%本文分别从发挥图书馆的馆藏职能、信息开发职能和教育职能入手,探讨如何提高职业学校图书馆资源配置的效果、效率和效能的途径。

  14. Resources.

    Science.gov (United States)

    Stewart, John; MacDonald, Ian

    1980-01-01

    Presents a guide to resources on television drama available to teachers for classroom use in television curriculum. Lists American and British television drama videorecordings of both series and individual presentations and offers a bibliography of "one-off" single fiction plays produced for British television. (JMF)

  15. Chromosome arm-specific BAC end sequences permit comparative analysis of homoeologous chromosomes and genomes of polyploid wheat

    Directory of Open Access Journals (Sweden)

    Sehgal Sunish K

    2012-05-01

    Full Text Available Abstract Background Bread wheat, one of the world’s staple food crops, has the largest, highly repetitive and polyploid genome among the cereal crops. The wheat genome holds the key to crop genetic improvement against challenges such as climate change, environmental degradation, and water scarcity. To unravel the complex wheat genome, the International Wheat Genome Sequencing Consortium (IWGSC is pursuing a chromosome- and chromosome arm-based approach to physical mapping and sequencing. Here we report on the use of a BAC library made from flow-sorted telosomic chromosome 3A short arm (t3AS for marker development and analysis of sequence composition and comparative evolution of homoeologous genomes of hexaploid wheat. Results The end-sequencing of 9,984 random BACs from a chromosome arm 3AS-specific library (TaaCsp3AShA generated 11,014,359 bp of high quality sequence from 17,591 BAC-ends with an average length of 626 bp. The sequence represents 3.2% of t3AS with an average DNA sequence read every 19 kb. Overall, 79% of the sequence consisted of repetitive elements, 1.38% as coding regions (estimated 2,850 genes and another 19% of unknown origin. Comparative sequence analysis suggested that 70-77% of the genes present in both 3A and 3B were syntenic with model species. Among the transposable elements, gypsy/sabrina (12.4% was the most abundant repeat and was significantly more frequent in 3A compared to homoeologous chromosome 3B. Twenty novel repetitive sequences were also identified using de novo repeat identification. BESs were screened to identify simple sequence repeats (SSR and transposable element junctions. A total of 1,057 SSRs were identified with a density of one per 10.4 kb, and 7,928 junctions between transposable elements (TE and other sequences were identified with a density of one per 1.39 kb. With the objective of enhancing the marker density of chromosome 3AS, oligonucleotide primers were successfully designed from

  16. L’iconostase de Rădeana-Bacău

    Directory of Open Access Journals (Sweden)

    Marina Sabados

    2010-01-01

    Full Text Available L’analyse complexe (peinture et sculpture décorative, iconographie et style de l’iconostase de Rădeana, dans le département de Bacău, un ensemble presque inconnu, donation du voïvode Gheorghe Ştefan de 1658, relève sa place singulière dans l’histoire moldave du genre. Au sujet de la peinture, les conclusions mènent vers un atelier galicien, tandis que la sculpture décorative à la manière baroque s’avère précoce par rapport aux ensembles contemporains (ceux ruthènes y compris et pourrait représenter le modèle, en tant qu’architecture et ornementation, des iconostases moldaves du XVIIIe siècle.

  17. library/mobile: Tips on Designing and Developing Mobile Web Sites

    OpenAIRE

    Hannah Gascho Rempel; Laurie M. Bridges; Kim Griggs

    2009-01-01

    Mobile applications can support learning by making library resources more ubiquitous, by bringing new users to the library through increased accessibility to the resources libraries offer, and by creating a new way to enhance connections between patrons and libraries. This increased use of mobile phones provides an untapped resource for delivering library resources to patrons. The mobile Web is the next step for libraries in providing universal access to resources and information. This articl...

  18. Direct Sensitivity Test of the MB/BacT System

    Directory of Open Access Journals (Sweden)

    Barreto Angela Maria Werneck

    2002-01-01

    Full Text Available In order to evaluate the direct-method test of sensitivity to drugs used in the principal tuberculosis treatment regimes, in the Organon Teknika MB/BacT system, we tested 50 sputum samples positive to microscopy taken from patients with pulmonary tuberculosis and with clinical indications for an antibiogram, admitted sequentially for examination during the routine of the reference laboratory. The material was treated v/v with 23% trisodium phosphate solution, incubated for 24 h at 35°C, and neutralized v/v with 20% monosodium phosphate solution. The material was then centrifuged and the sediment inoculated into flasks containing Rifampin - 2 µg/ml, Isoniazid - 0.2 µg/ml, Pyrazinamide - 100 µg/ml, Ethambutol - 2.5 µg/ml, Ethionamide - 1.25 µg/ml, and Streptomycin - 2 µg/ml. The tests were evaluated using the indirect method in the BACTEC 460 TB (Becton Dickinson system as the gold standard. The results showed that the Rifampin test performed best, i.e., 100% sensitivity at 95% Confidence Interval (82.2-100 and 100% specificity at 95% Confidence Interval (84.5-100, followed by Isoniazid and Pyrazinamide. In this experiment, 92% of the materials showed a final reading in 30 days; this period represents the time for primary isolation as well as the results of the sensitivity profile, and is within Centers for Disease Control and Prevention recommendations regarding time for performance of the antibiogram. The inoculated flasks showed no contamination during the experiment. The MB/BacT is shown to be a reliable, rapid, fully automated nonradiometric system for the tuberculosis antibiogram.

  19. Building the Digital Branch A Library Technology Report

    CERN Document Server

    King, David Lee

    2009-01-01

    This issue of Library Technology Reports explores the idea of the digital branch-a library website that is a vital, functional resource for patrons and enhances the library's place within its community.

  20. CRNL library serials list

    International Nuclear Information System (INIS)

    A list of 1900 serial publications (periodicals, society transactions and proceedings, annuals and directories, indexes, newspapers, etc.) is presented with volumes and years held by the Main Library. This library is the largest in AECL as well as one of the largest scientific and technical libraries in North America, and functions as a Canadian resource for nuclear information. A main alphabetical list is followed by broad subject field lists representing research interests, and lists of abstract and index serials, general bibliographic serials, conference indexes, press releases, English translations, and original language journals

  1. 提高大学图书馆数字资源的利用率——以山东农业大学图书馆为例%Improving Digital Resource Utilization Rate in University Libraries ---Taking the Library of Shandong Agricultural University as an example

    Institute of Scientific and Technical Information of China (English)

    何凤丽

    2012-01-01

    Social responsibility of library lies in providing excellent service to the readers. Development and utilization of digi- tal resource in the university library is one important component in today' s library routine work. We take the li- brary in Shandong Agricultural University as an example to investigate and analysis the digital resource situation in utilization by a questionnaire. There existed low utilizations on most digital resource, great utilization differences a- mong data bases and huge anticipations on digital resources services from students. Finally, some advices on digital resource development, utilization and information education were proposed in the expectation to be beneficial for digital resource utilization in university libraries%图书馆的社会责任体现在给读者提供优质的服务。数字资源的建设和利用时当前图书馆重要组成部分,我们以山东农业大学图书馆为例,调查与分析大学生对该馆拥有的数字资源的使用情况,发现目前图书馆普遍存在馆藏数字资源的利用率相对较低、数据库使用差异较大和大学生对数字资源和服务的建设有较高要求等,在资源建设、使用和教育方面提出了建议和措施。

  2. Libraries in Washington: MedlinePlus

    Science.gov (United States)

    ... 10th St. Anacortes, WA 98221 360-293-1910 ext. 24 http://library.cityofanacortes.org Bellevue Overlake Hospital ... Suite 580 Seattle, WA 98116 206-860-0329 ext. 22 http://www.xculture.org/resource/library/index. ...

  3. Libraries in Alaska: MedlinePlus

    Science.gov (United States)

    ... www.cityofhomer-ak.gov/library Ketchikan Ketchikan Public Library 629 Dock St. Ketchikan, AK 99901 907-225-3331 http://www.firstcitylibraries.org/KPLhomepage.html Soldotna Central Peninsula Hospital Health Resource Center 250 Hospital Place ...

  4. Welcome to the National Wetlands Research Center Library: Not Just Another Library-A Special Library

    Science.gov (United States)

    Broussard, Linda

    2007-01-01

    Libraries are grouped into four major types: public, school, academic, and special. The U.S. Geological Survey's (USGS) National Wetlands Research Center (NWRC) library is classified as a special library because it is sponsored by the Federal government, and the collections focus on a specific subject. The NWRC library is the only USGS library dedicated to wetland science. Library personnel offer expert research services to meet the informational needs of NWRC scientists, managers, and support personnel. The NWRC library participates in international cataloging and resource sharing, which allows libraries from throughout the world to borrow from its collections. This sharing facilitates the research of other governmental agencies, universities, and those interested in the study of wetlands.

  5. 武警院校多媒体资源库建设的现状及对策%The status quo and countermeasures of multimedia resources library construction in armed police force academies

    Institute of Scientific and Technical Information of China (English)

    何建鹏; 王基生

    2011-01-01

    Based on the survey results of multimedia resources library construction in related academies amongtechnieal staff participating in armed police force educational technology training, the paper tries to identify the main factors affecting and constraining the construction of academic multimedia resources library. It also brings forward some countermeasures and suggestions for multimedia resources construction in order to improve the teaching support of multimedia resources library and provide better services for multimedia-assisted teaching.%通过对参加武警部队教育技术骨干培训的人员进行有关院校多媒体教育资源建设现状的调查,分析了影响和制约多媒体资源库建设的主要因素,提出了对策与建议,以提高多媒体资源库的教学保障能力,更好地为多媒体教学服务.

  6. Investigation on the Construction of Digital Resources in Libraries of Mining Colleges and Universities%矿业类高校图书馆数字资源建设调查研究

    Institute of Scientific and Technical Information of China (English)

    李红梅

    2014-01-01

    通过访问11所隶属于原煤炭部的矿业类高等院校的图书馆网站,对其数据库的数量、引进数据库、自建数字资源以及数字资源的组织方式等进行了统计分析,以期能够反映出我国矿业类高等院校图书馆数字资源建设与利用情况,为矿业类院校图书馆在数字资源建设与利用方面相互借鉴提供参考。%Through accessing to the websiets of the libraries of 11 coal mining colleges and universities affiliated with the former Ministry of Coal Industry, this paper makes a statistical analysis on database number, introduces database, self-built digital resources, and the organization form of digital resources, etc., hoping to reflect the current construction and utilization of digital resources in the libraries of coal mining colleges and universities in China so as to provide a reference in the construction and utilization of digital resources in the libraries of coal mining colleges and universities .

  7. 高校图书馆学科信息资源建设存在的问题及对策%The Issues and the Corresponding Solutions in the Construction of University Library Information Resource System

    Institute of Scientific and Technical Information of China (English)

    曾咏梅

    2011-01-01

    the library information resource system is an important part of the construction of university discipline construction. According to the current situation of the construction of library information resource system, the research gave the reason analysis from the aspects of resource quantity, the Subject Librarians system, the resource integration, the department integration and funding support. Meanwhile, the corresponding resolutions were also suggested, which could offer a certain degree reference to university libraries%学科信息资源建设是高校学科建设中的重要组成部分,针对高校学科信息资源建设现状.对馆藏资源、学科馆员、整合平台、部门配合、专项经费等几方面的问题进行了分析,并提出相应对策,为相关高校提供参考。

  8. 省级公共图书馆文献资源政府采购探析%An Inquiry of Government Procurement of Literature Resources in Provincial Public Library

    Institute of Scientific and Technical Information of China (English)

    赵迎春

    2011-01-01

    文献资源的政府采购,是省级公共图书馆文献资源采购的发展方向。作者通过电话调研的方式,了解了港、澳、台之外的全国32个省级公共图书馆的文献资源采购情况,并对省级公共图书馆文献资源政府采购进行了分析。%The government procurement of literature resources is an important direction of development on literature resources procurement in provincial public library. To know more, the author makes an inquiry on the purchase of literature resources among 32 national provincial public libraries besides Hong Kong, Macau and Taiwan by telephone inquiry. This article is an analysis after investigating on government procurement of literature resources in provincial public libraries.

  9. 面向实习用户的图书馆数字资源远程访问实现探究%On the Remote Access to Digital Resources of Library Oriented to Practice Users

    Institute of Scientific and Technical Information of China (English)

    曾小莹

    2012-01-01

    通过对高职院校实习师生用户文献信息需求的调查,图书馆为实习用户服务的现状分析,提出利用SSL-VPN、反向代理、3G手机图书馆等技术实现图书馆数字资源远程访问,以满足实习用户的文献信息需求。%This paper make an investigation of the demands for documents information of practice users,analyses the status of library service for practice users,and puts forward the realization of remote access to digital resources of li-brary by using the vpn technology,the reverse proxy technology,the 3G mobile library to meet the document information needs of users.

  10. Conversion of BAC clones into binary BAC (BIBAC) vectors and their delivery into basidiomycete fungal cells using Agrobacterium tumefaciens.

    Science.gov (United States)

    Ali, Shawkat; Bakkeren, Guus

    2015-01-01

    The genetic transformation of certain organisms, required for gene function analysis or complementation, is often not very efficient, especially when dealing with large gene constructs or genomic fragments. We have adapted the natural DNA transfer mechanism from the soil pathogenic bacterium Agrobacterium tumefaciens, to deliver intact large DNA constructs to basidiomycete fungi of the genus Ustilago where they stably integrated into their genome. To this end, Bacterial Artificial Chromosome (BAC) clones containing large fungal genomic DNA fragments were converted via a Lambda phage-based recombineering step to Agrobacterium transfer-competent binary vectors (BIBACs) with a Ustilago-specific selection marker. The fungal genomic DNA fragment was subsequently successfully delivered as T-DNA through Agrobacterium-mediated transformation into Ustilago species where an intact copy stably integrated into the genome. By modifying the recombineering vector, this method can theoretically be adapted for many different fungi. PMID:25239747

  11. BacPP: a web-based tool for Gram-negative bacterial promoter prediction.

    Science.gov (United States)

    de Avila E Silva, S; Notari, D L; Neis, F A; Ribeiro, H G; Echeverrigaray, S

    2016-01-01

    Bacterial Promoter Prediction (BacPP) is a tool used to predict given sequences as promoters of Gram-negative bacteria according to the σ factor that recognizes it. The first version of BacPP was implemented in Python language in a desktop version without a friendly interface. For this reason, a web version of BacPP is now available with the purpose of improving its usability and availability. The present paper describes the implementation of the web version of this tool, focusing on its software architecture and user functionalities. The software is available at www.bacpp.bioinfoucs.com/home. PMID:27173187

  12. Library Cooperation at the Grass Roots: A Regional Intertype Library Network in the San Joaquin Valley.

    Science.gov (United States)

    Galloway, R. Dean

    1979-01-01

    A small academic library's experience in a regional intertype library network had positive effects on interlibrary loan procedures and improved and promoted cooperation at the local level. The regional network relieves the burden on large research libraries and enables libraries with inadequate resources to provide better service to patrons.…

  13. 基于SWOT分析的高校图书馆数字资源云存储模式探讨%The SWOT Analysis of the Digital Resources of University Library Based on Cloud Storage

    Institute of Scientific and Technical Information of China (English)

    彭文

    2014-01-01

    First elaborated the current situation of library digital resources storage, problems and future resource storage requirements, on this basis, using swot analysis of cloud storage brings to the University Library opportunities and threats, as well as the application of cloud storage library's own advantages and disadvantages, and finally the formation of swot analysis matrix, and puts forward four kinds of strategies of University Library Application cloud development of storage.%本文首先阐述了目前图书馆数字资源存储现状、面临的问题和未来资源存储需求,在此基础上,采用SWOT方法分析云存储给高校图书馆带来的机遇和威胁,以及图书馆自身应用云存储的优势和劣势,最后形成SWOT分析矩阵,并提出高校图书馆应用云存储发展的四种战略对策。

  14. On the Cooperative Preservation of Printed Documentary Resources in the Central-Branch Libraries System%总分馆体制下印刷型文献资源协同保存探讨

    Institute of Scientific and Technical Information of China (English)

    罗婉娜

    2013-01-01

    通过对图书馆印刷型文献馆藏现状、总分馆体制发展趋势的分析,结合国内外图书馆多年实践经验,以东莞为例,探讨图书馆在总分馆体制管理架构下,以协同保存的方式解决区域内图书馆不断增长的印刷型文献的保存、利用问题。%Through analysis of the current collection of printed documentary resources and the development situations of central-branch libraries system , and of the experience of domestic and foreign libraries for years , this paper, taking Dongguan as an example, probes into how to solve the problems of the preservation and utilization of the increasingly large number of printed docu -mentary resources in the regional library under the central-branch libraries system with a cooperative preservation mode .

  15. Discussion on the Construction of Automotive Kind of Specialty Teaching Resources Library in Vocational Colleges%高职院校汽车类专业教学资源库建设的研究

    Institute of Scientific and Technical Information of China (English)

    潘伟荣; 张飞; 郭海龙

    2012-01-01

    文中分析了高职院校汽车专业教学资源建设的现状,以工作过程系统化课程开发思想为指导,以专业建设为基础,课程为核心,对构建高职院校汽车类专业教学资源库的系统框架和内容进行了研究,尝试构建汽车行业信息库、专业建设标准库、课程资源库、培训资源库、素材库和特色资源库的系统模型及内容,同时研究了专业数据库平台管理机制,保证优质教学资源适应用户的多种应用需求功能,促进学生主动式、协作式、研究型、自主型的学习教学改革,不断提高高职教学质量。%This paper analyzes the status of t teaching resource library(e.g.hardware,software or database) construction of Automotive Specialty in Vocational colleges.Systematic work process of curriculum development as guidance,Professional development,curriculum for the core,Build the framework and content of vocational colleges auto major teaching resource library system.Try to build the information base of the automotive industry,specializing in the construction of the standard library,curriculum resource library,the library of training resources,material libraries,and features of the repository system model and content.at the same time research professional database platform management mechanismto ensure quality teaching resources to adapt to a variety of application needs of the user function,and promote students' active,collaborative,research-oriented,self-learning teaching reform,and constantly improve the quality of vocational teaching.

  16. Dynamic teaching resource library for measurement and control technology based on ASP%基于 ASP 技术的动态测控专业教学资源库建设

    Institute of Scientific and Technical Information of China (English)

    陈庆春; 郭俊美

    2014-01-01

    网络教学库可以满足学生自主学习的需要,增加学生学习的积极主动性,有利于促进教学效果和教学质量的提高。本文介绍了以Dreamweaver作为开发工具,采用ASP技术,以Access数据库作为后台数据库的动态测控专业教学资源库的设计与实现。本教学资源库主要包括前台和后台两部分。前台面向普通用户,主要实现课程资源、实验资源及教学软件的浏览、下载以及留言。后台面向管理员,主要实现教学资源及留言信息的管理。本教学资源库具有资源共享、系统开放、灵活生动等优点。%The teaching resource library can satisfy the students'autonomous learning to increase the students'initiative.As a result, teaching effect and teaching quality will be improved.Design and implementation of the dynamic teaching resource library about measurement and control technology are introduced in this article.Dreamweaver,as a development tool,ASP and Access database are applied.The teaching resource library has two parts,foreground and background .The foreground is for common users to browse and download all resources such as course resources , experiment resources and instructional software ,and leave one's comments .Thebackground is for the ad ministrator to manage the resources and comments.The teaching resource library has some advantages such as it is open for the users to login it with sharing resources ,and is lively and interesting .

  17. Co-construction and Sharing of Collection Resources among Medical University and Local Hospital Libraries%高等医学院校与地方医院馆藏资源的共建共享

    Institute of Scientific and Technical Information of China (English)

    赵旦

    2009-01-01

    The paper discusses the necessity and the feasibility of co - construction and sharing of collection resources among medical university and local hospital libraries, proposes concrete measures of its implementing, including cultivating overall situation awareness, strengthening resources construction and highlighting the characteristics of collections and implementing unified library card.%论述高等医学院校与地方医院图书馆资源共建共享的必要性和可行性,提出具体实施措施,包括树立大局意识、加强资源建设突出特色馆藏、实施通用借书证等.

  18. Application of Modern Human Resource Management and Development to the Library%现代人力资源管理与开发理论在图书馆中的应用

    Institute of Scientific and Technical Information of China (English)

    秦巧春

    2011-01-01

    从经典人力资源管理与开发理论人手,通过对企业与图书馆在理论应用中存在的人文环境、机构性质、人才结构、成本投入等差别的分析,图书馆要在差异中寻求同一,探索并建立符合自身特点和实践需要的人力资源管理与开发理论体系。%Starting with the classic theory of human resource management and development, and based on the difference analysis between businesses and libraries in the aspects as human environment, institutional properties, personnel structure, cost, etc. it is proposed that libraries are to seek the same in differences and to explore the human resources management and development with its own characteristics.

  19. 基于云计算的图书馆信息资源共享系统架构研究%On the Architecture of Library Information Resource Sharing Systems Based on Cloud Computing

    Institute of Scientific and Technical Information of China (English)

    陈玲洪

    2012-01-01

    The concept,characteristics and classification of cloud computing are introduced,and the author analyzes the impact of cloud computing on library information resource sharing systems.On this basis,the author designs an architecture model of library information resource sharing system based on cloud computing.%对云计算的概念、特点和分类进行了介绍,并且分析了云计算技术对图书馆信息资源共享系统带来的影响,在此基础之上设计了基于云计算的图书馆信息资源共享系统的架构模型。

  20. Genome evolution in Reptilia: in silico chicken mapping of 12,000 BAC-end sequences from two reptiles and a basal bird

    Directory of Open Access Journals (Sweden)

    Edwards Scott V

    2009-07-01

    Full Text Available Abstract Background With the publication of the draft chicken genome and the recent production of several BAC clone libraries from non-avian reptiles and birds, it is now possible to undertake more detailed comparative genomic studies in Reptilia. Of interest in particular are the genomic events that transformed the large, repeat-rich genomes of mammals and non-avian reptiles into the minimalist chicken genome. We have used paired BAC end sequences (BESs from the American alligator (Alligator mississippiensis, painted turtle (Chrysemys picta and emu (Dromaius novaehollandiae to investigate patterns of sequence divergence, gene and retroelement content, and microsynteny between these species and chicken. Results From a total of 11,967 curated BESs, we successfully mapped 725, 773 and 2597 sequences in alligator, turtle, and emu, respectively, to sites in the draft chicken genome using a stringent BLAST protocol. Most commonly, sequences mapped to a single site in the chicken genome. Of 1675, 1828 and 2936 paired BESs obtained for alligator, turtle, and emu, respectively, a total of 34 (alligator, 2%, 24 (turtle, 1.3% and 479 (emu, 16.3% pairs were found to map with high confidence and in the correct orientation and with BAC-sized intermarker distances to single chicken chromosomes, including 25 such paired hits in emu mapping to the chicken Z chromosome. By determining the insert sizes of a subset of BAC clones from these three species, we also found a significant correlation between the intermarker distance in alligator and turtle and in chicken, with slopes as expected on the basis of the ratio of the genome sizes. Conclusion Our results suggest that a large number of small-scale chromosomal rearrangements and deletions in the lineage leading to chicken have drastically reduced the number of detected syntenies observed between the chicken and alligator, turtle, and emu genomes and imply that small deletions occurring widely throughout the

  1. 国内高校图书馆资源共享平台建设探析%On the Construction of Resource Sharing Platform in University Libraries in China

    Institute of Scientific and Technical Information of China (English)

    张展

    2015-01-01

    目前我国高校图书馆资源共享平台的建设还处于一个起步阶段,建设过程中存在着诸如平台建设参差不齐、共享内容不够全面、平台建设主要依靠数据商、没有建立绩效评价体系等一系列问题。我们需要加强资源建设,进一步扩大共享范围,提升自身形象,强化图书馆在平台建设中的作用,寻求政府支持,完善平台协调管理机制等。%The current university library resources sharing platform in China is still in an early stage of develop-ment.There are still a host of problems facing the construction of resource sharing platform in Chinese university li-braries, such as uneven development in platform construction among different universities, incomprehensive re-sources that can be shared, lop-sided reliance on data business providers, lack of performance evaluation systems, et cetera.To solve these problems, efforts must be made to strengthen library resource building, further expand the scope of sharing, strengthen the role of university libraries in platform construction, enhance libraries’ self-image, seek government support, and improve the mechanism of platform coordination and management.

  2. Library statistics.

    OpenAIRE

    Melita Ambrožič

    1991-01-01

    The contribution deals with the purpose, beginnings and development of library statistics and the strivings for international standardization in this field. International recommendations are presented, as well as the ISO standard for library statistics. An overview is given of the theoretical contributions and statistical practice in Slovenian librarianship. Cautionary notice on the limitations of the applicability of library statistics in determining library performance is given and the inte...

  3. Library Outreach: Introducing Campus Childcare Providers to the Academic Library

    Science.gov (United States)

    Edwards, Melissa Maxwell; Thornton, Elaine

    2013-01-01

    This case study describes a library outreach effort to university staff members employed by the campus child care center. Authors planned an instructional session to introduce child care staff members to library resources, focusing on the curriculum collection as a source of supplemental materials for classrooms. Surveys were administered before…

  4. 高校数字图书馆信息资源管理的问题及对策%Problems and countermeasures of information resource management of university digital library

    Institute of Scientific and Technical Information of China (English)

    林平

    2014-01-01

    信息是现代社会的战略资源,是市场竞争的重要手段。数字图书馆是传统图书馆在信息时代的发展,是数字技术、网络技术发展的产物,是未来社会公共信息的中心和枢纽。数字图书馆是现阶段我国一项新兴技术产业,在建设、运用等方面取得一定的成就,但与国外欧美国家的数字图书馆发展相比,仍存在较多的问题和不足。我国数字图书馆的发展现状是?如何促进我国数字图书馆的发展?本文主要从图书信息资源管理的角度,分析了高校数字图书馆在管理中存在的问题,及其相关解决对策。%Information is the strategic resources of modern society, it is an important mean of market competition. Digital library is the development of traditional library in the information era, is a product of the development of network technology and digital technology, is the center and hub of future social public information. The digital library in our country currently is an emerging technology industry, has made some achievements in construction, such as the use of digital library development, but compared with foreign countries in Europe and America, still existing problems and defects. What is the development status of digital library in China? How to promote the development of digital library in China? This article mainly from the angle of information resource management in library, to analyze the existing problems in the management of university digital library, and the related countermeasures.

  5. The Building Research of the Share-based Teaching Resources Library of International Shipping Business Management%国际航运业务管理专业共享型教学资源库建设研究

    Institute of Scientific and Technical Information of China (English)

    涂建军

    2011-01-01

    文中基于国家骨干高职院校建设共享型专业教学资源库的任务需求,结合广东交通职业技术学院国际航运业务管理专业的实际情况,从建设意义、设计理念、建设内容、建设对策四方面对如何建设国际航运业务管理专业教学资源库平台进行了详细研究,为资源库平台的实施提供了参考依据和理论支持。%The paper introduces the research of how to build the Share-based teaching resources library platform of International shipping business management in detail, based on the mission requirements of the national vocational colleges building the share-based teaching resource library. It includes four aspects, such as the building significance, the designing concept, the building elements, the building measures and so on. The research provides the reference and theory support on how to carry out the building of the Share-based teaching resources library.

  6. Practicability Research of Professional Courses Teaching Re-source Library Building%高职专业课教学资源库建设的实用性研究

    Institute of Scientific and Technical Information of China (English)

    王楠

    2015-01-01

    数字化校园的建立,给高职专业课教学资源库建设提供了坚实的技术支撑.怎样建设、充实教学资源库,让资源库服务教学发挥正能量作用,应作为数字化校园实用性研究的重点方向,目标是能充实完善专业资料,提供丰富的开放信息,引导开拓使用项目,真正体现专业课教学资源库的价值.%Establish digital campus and popularize campus network, has become a solid technical support for teaching resource library of professional courses in high vocational education. The main direction of practicability research for digital campus is how to build, enrich and make positive use of teaching resource library, with the aim of completing professional data, offering abundant open information, giving guidance to exploit projects and truly embodying the value of teaching resource library professional courses.

  7. Study on Logistics Information Technology Teaching Resource Library Based on Logistics Business Processes%基于物流业务流程的物流信息技术专业教学资源库的研究

    Institute of Scientific and Technical Information of China (English)

    杨晓峰; 张茁

    2015-01-01

    文中阐述了如何应用面向对象的教学资源库设计思想建设基于物流业务流程的物流信息技术专业教学资源库,详细阐述了运用先进“互联网+”现代信息技术,融入云计算服务模式,采用分布式布局的网络体系架构建设物流信息技术专业教学资源库的设计思路和方案。%This paper describes how to use teaching resource library design construction of object-oriented based logistics business processes of logistics IT teaching resource library,elaborated on the use of advanced “Internet+”modern information technology into the cloud services model,using network architecture distributed layout construction of logistics IT professional teaching resource library of design ideas and programs.

  8. Digital Libraries

    CERN Document Server

    Papy, Fabrice

    2008-01-01

    Of vital interest to all librarians and information specialists, this book presents all aspects of the effects of digitization of today's and tomorrow's libraries. From social to technical issues, Digital Libraries includes chapters on the growth of the role of librarian, the reader experience, cataloging, search engines, OPAC, law, ergonomic studies, and the future of libraries.

  9. A first generation BAC-based physical map of the channel catfish genome

    Directory of Open Access Journals (Sweden)

    Waldbieser Geoffrey C

    2007-02-01

    Full Text Available Abstract Background Channel catfish, Ictalurus punctatus, is the leading species in North American aquaculture. Genetic improvement of catfish is performed through selective breeding, and genomic tools will help improve selection efficiency. A physical map is needed to integrate the genetic map with the karyotype and to support fine mapping of phenotypic trait alleles such as Quantitative Trait Loci (QTL and the effective positional cloning of genes. Results A genome-wide physical map of the channel catfish was constructed by High-Information-Content Fingerprinting (HICF of 46,548 Bacterial Artificial Chromosomes (BAC clones using the SNaPshot technique. The clones were assembled into contigs with FPC software. The resulting assembly contained 1,782 contigs and covered an estimated physical length of 0.93 Gb. The validity of the assembly was demonstrated by 1 anchoring 19 of the largest contigs to the microsatellite linkage map 2 comparing the assembly of a multi-gene family to Restriction Fragment Length Polymorphism (RFLP patterns seen in Southern blots, and 3 contig sequencing. Conclusion This is the first physical map for channel catfish. The HICF technique allowed the project to be finished with a limited amount of human resource in a high throughput manner. This physical map will greatly facilitate the detailed study of many different genomic regions in channel catfish, and the positional cloning of genes controlling economically important production traits.

  10. Investigation on the Organization of Open Access Resources in Chinese Public Libraries%我国公共图书馆揭示开放获取资源现状调查

    Institute of Scientific and Technical Information of China (English)

    蒋静

    2014-01-01

    文章调查分析了我国62家公共图书馆对于开放获取资源的揭示情况,分析其成果及存在的问题,并提出了未来的发展策略。%The article investigates the organization of open access resources of 62 public libraries in China, then analyzes its achievements and problems, and puts forward some strategies for improving collection development using open access resources.

  11. 基于用户需求的馆藏期刊资源的优化配置%The Optimal Allocation of Library Journal Resources Based on User Needs

    Institute of Scientific and Technical Information of China (English)

    尚艳秋

    2015-01-01

    Periodical resources allocation related to the building of library resources, In the present case of the database widely using, It is the subject of this article:how rational allocation of print journals and electronic journals, and satisfy the needs of readers.%期刊资源配置关系到馆藏资源建设,在目前数据库广泛使用情况下,如何合理配置印刷型和电子期刊,最大限度地满足读者的需求,是文章探讨的问题。

  12. Is BAC Transgenesis Obsolete? State of the Art in the Era of Designer Nucleases

    Directory of Open Access Journals (Sweden)

    J. Beil

    2012-01-01

    Full Text Available DNA constructs based on bacterial artificial chromosomes (BACs are frequently used to generate transgenic animals as they reduce the influence of position effects and allow predictable expression patterns for genes whose regulatory sequences are not fully identified. Despite these advantages BAC transgenics suffer from drawbacks such as complicated vector construction, low efficiency of transgenesis, and some remaining expression variegation. The recent development of transcription activator-like effector nucleases (TALENs and zinc finger nucleases (ZFNs has resulted in new transgenic techniques which do not have the drawbacks associated with BAC transgenesis. Initial reports indicate that such designer nucleases (DNs allow the targeted insertion of transgenes into endogenous loci by direct injection of the targeting vector and mRNA/DNA encoding the predesigned nucleases into oocytes. This results in the transgene being inserted at a specific locus in the mouse genome, thus circumventing the drawbacks associated with BAC transgenesis.

  13. The Paths and Countermeasures for the Development and Application of Dissertation Resources in University Library%高校图书馆开发与应用学位论文资源的途径及对策

    Institute of Scientific and Technical Information of China (English)

    于东琴

    2015-01-01

    The dissertation resources, which are an essential part of university library’s collection, belong to a kind of key information resources for university’s research and teaching. This paper analyzes the features and information value of university’s dissertation resources, expounds the importance for university library to develop and apply the dissertation resources, and probes into the paths and countermeasures for the development and application of the dissertation resources in university library.%高校图书馆馆藏的必不可少的组成部分是学位论文资源,它属于高校科研与教学的一种关键性信息资源。分析了高校学位论文的特点和情报价值,阐述了高校图书馆开发与应用学位论文资源的重要性,探讨了高校图书馆开发与应用学位论文资源的途径及策略。

  14. Present Situation and Countermeasure for the Utilization of University Libraries' Digital Resources%试论高校图书馆电子资源利用现状与对策

    Institute of Scientific and Technical Information of China (English)

    张俊

    2012-01-01

    高校图书馆电子资源存在宣传和培训工作不足、电子资源的质量和功能参差不齐、电子资源的网络配置不足等问题,要求我们通过多种途径,采取多种方法来整合电子资源,加强如何有效利用电子资源的宣传和培训工作,变无序为有序,完善网络环境,提升检索效果,最终提高电子资源的利用率。%There are some probloms in the utilization of university libraries' digital resources,such as insufficient advocacy and training,uneven quality and function of digital resources,lack of network configuration and so on.So we should put forward some countermeasures for increasing the utilization ratio of the digital resources of university library,such as using some ways to strengthen the publicity and training of electronic resources,integrating digital resources,and changing the disorder to order,improving the network environment and search results.

  15. PiggyBac Transposon Mutagenesis: A Tool for Cancer Gene Discovery in Mice

    OpenAIRE

    Rad, Roland; Rad, Lena; Wang, Wei; Cadinanos, Juan; Vassiliou, George; Rice, Stephen; Campos, Lia S.; Yusa, Kosuke; Banerjee, Ruby; Li, Meng Amy; de la Rosa, Jorge; Strong, Alexander; Lu, Dong; Ellis, Peter; Conte, Nathalie

    2010-01-01

    Transposons are mobile DNA segments that can disrupt gene function by inserting in or near genes. Here we show that insertional mutagenesis by the PiggyBac transposon can be used for cancer gene discovery in mice. PiggyBac transposition in genetically engineered transposon/transposase mice induced cancers whose type (hematopoietic versus solid) and latency were dependent on the regulatory elements introduced into transposons. Analysis of 63 hematopoietic tumors revealed the unique qualities o...

  16. Libraries Serving the CSIR Complex.

    Science.gov (United States)

    Rajagopalan, T. S.; Ramaswami, K.

    A survey of the resources and services of the Council of Scientific and Industrial Research (CSIR) libraries was made so that the libraries in the complex could share the benefit of the experiences of each other. The report is based on questionnaire replies received from 31 CSIR Institutions and eight Co-operative Research Associations and relates…

  17. JCE Digital Library Grand Opening

    Science.gov (United States)

    Journal of Chemical Education, 2004

    2004-01-01

    The National Science, Technology, Engineering and Mathematical Education Digital Library (NSDL), inaugurated in December 2002, is developed to promote science education on a comprehensive scale. The Journal of Chemical, Education (JCE) Digital Library, incorporated into NSDL, contains its own collections of digital resources for chemistry…

  18. The Weakest Link: Library Catalogs.

    Science.gov (United States)

    Young, Terrence E., Jr.

    2002-01-01

    Describes methods of correcting MARC records in online public access catalogs in school libraries. Highlights include in-house methods; professional resources; conforming to library cataloging standards; vendor services, including Web-based services; software specifically developed for record cleanup; and outsourcing. (LRW)

  19. Hybrid nonviral/viral vector systems for improved piggyBac DNA transposon in vivo delivery.

    Science.gov (United States)

    Cooney, Ashley L; Singh, Brajesh K; Sinn, Patrick L

    2015-04-01

    The DNA transposon piggyBac is a potential therapeutic agent for multiple genetic diseases such as cystic fibrosis (CF). Recombinant piggyBac transposon and transposase are typically codelivered by plasmid transfection; however, plasmid delivery is inefficient in somatic cells in vivo and is a barrier to the therapeutic application of transposon-based vector systems. Here, we investigate the potential for hybrid piggyBac/viral vectors to transduce cells and support transposase-mediated genomic integration of the transposon. We tested both adenovirus (Ad) and adeno-associated virus (AAV) as transposon delivery vehicles. An Ad vector expressing hyperactive insect piggyBac transposase (iPB7) was codelivered. We show transposase-dependent transposition activity and mapped integrations in mammalian cells in vitro and in vivo from each viral vector platform. We also demonstrate efficient and persistent transgene expression following nasal delivery of piggyBac/viral vectors to mice. Furthermore, using piggyBac/Ad expressing Cystic Fibrosis transmembrane Conductance Regulator (CFTR), we show persistent correction of chloride current in well-differentiated primary cultures of human airway epithelial cells derived from CF patients. Combining the emerging technologies of DNA transposon-based vectors with well-studied adenoviral and AAV delivery provides new tools for in vivo gene transfer and presents an exciting opportunity to increase the delivery efficiency for therapeutic genes such as CFTR. PMID:25557623

  20. Measurement Properties of the Low Back Activity Confidence Scale (LoBACS).

    Science.gov (United States)

    Davenport, Todd E; Cleland, Joshua A; Yamada, Kimiko A; Kulig, Kornelia

    2016-06-01

    The purpose of this study was to determine the measurement properties of the Low Back Activity Confidence Scale (LoBACS) in individuals with post-acute low back pain (LBP) receiving nonsurgical intervention, including construct validity, factorial validity, and internal consistency reliability. Data were analyzed from an existing randomized clinical trial involving 112 patients with LBP. Evidence for convergent validity was observed through significant correlations between LoBACS subscale scores and other function, pain, and psychobehavioral measures. LoBACS subscales accounted for 36% of the unique variance in dependent variable measurements, suggesting a satisfactory level of statistical divergence between the LoBACS and other psychobehavioral measurements in this study. Cronbach's α ranged from .88 to .92 for LoBACS subscales, and item-total correlations exceeded .6, indicating high internal consistency reliability. Principal axis factoring confirmed the hypothesized three-subscale structure by correctly classifying 14 of the 15 items. These findings indicate the LoBACS is valid and internally consistent to measure domain-specific self-efficacy beliefs. PMID:24686745