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Sample records for azolla filiculoides colectadas

  1. An ecophysiological study of the Azolla filiculoides- Anabaena azollae association

    Science.gov (United States)

    van Kempen, Monique; Smolders, Fons; Speelman, Eveline; Reichart, Gert Jan; Barke, Judith; Brinkhuis, Henk; Lotter, Andy; Roelofs, Jan

    2010-05-01

    The long term effects of salinity stress on the growth, nutrient content and amino acid composition of the Azolla filiculoides - Anabaena azollae association was studied in a laboratory experiment. It was demonstrated that the symbiosis could tolerate salt stress up to 90 mM NaCl, even after a 100 day period of preconditioning at salt concentrations that were 30 mM NaCl lower. In the 120 mM NaCl treatment the Azolla filiculoides survived, but hardly any new biomass was produced. It was shown that during the experiment, A. filiculoides became increasingly efficient in excluding salt ions from the plant tissue and was thus able to increase its salt tolerance. The amino acid analysis revealed that the naturally occurring high glutamine concentration in the plants was strongly reduced at salt concentrations of 120 mM NaCl and higher. This was the result of the reduced nitrogenase activity at these salt concentrations, as was demonstrated in an acetylene reduction assay. We suggest that the high glutamine concentration in the plants might play a role in the osmoregulatory response against salt stress, enabling growth of the A. filiculoides -Anabaena azollae association up to 90 mM NaCl. In a mesocosm experiment it furthermore was demonstrated that Azolla might manipulate its own microenvironment when grown at elevated salt concentration (up to ~50 mmol•L-1) by promoting salinity stratification, especially when it has formed a dense cover at the water surface. Beside salt stress, we also studied the growth of Azolla filiculoides in response to elevated atmospheric carbon dioxide concentration, in combination with different light intensities and different pH of the nutrient solution. The results demonstrated that as compared to the control (ambient pCO2 concentrations), Azolla filiculoides was able to produce twice as much biomass at carbon dioxide concentrations that were five times as high as the ambient pCO2 concentration. However, it was also shown that this

  2. Clonal reproduction of Azolla filiculoides Lam.: implications for invasiveness

    OpenAIRE

    Fernández Zamundio, María del Rocio; Cirujano Bracamonte, Santos; Meco, Ana; García Murillo, Pablo

    2013-01-01

    Clonal reproduction of Azolla filiculoides Lam., an aquatic floating fern, native from the American subtropics and a recent invader of Mediterranean wetlands, was assessed experimentally. Shoot fragmentation of the species was quantified under suitable room conditions for optimum growth. Azolla fililculoides showed high longevity, a low mortality percentage and a high potential for clonal reproduction (biomass obtained by shoot fragmentation from only one individual was multiplied ...

  3. Azolla filiculoides Nitrogenase Activity Decrease Induced by Inoculation with Chlamydomonas sp. †

    OpenAIRE

    Habte, Mitiku

    1986-01-01

    Experiments were conducted to determine the influence of Chlamydomonas sp. on nitrogen fixation (C2H2 → C2H4) in Azolla filiculoides and on the nitrogen fixation and growth of free-living Anabaena azollae 2B organisms. Inoculation of azolla medium with Chlamydomonas sp. was associated with decreased nitrogenase activity in A. filiculoides and with increases in the density of a fungal population identified as Acremonium sp. Subsequent inoculation of azolla medium with this fungus was also acco...

  4. Distribution of Azolla filiculoides Lam. (Azollaceae in Poland

    Directory of Open Access Journals (Sweden)

    Ewa Szczęśniak

    2011-04-01

    Full Text Available Azolla filiculoides has been an ephemeral plant in Poland since the end of the 20th century. In the last 15 years this species appeared in 5 locations in south-west Poland. Habitat and plants of two populations became destroyed, three other still exist. A. filiculoides occurs in eutrophic or even polluted water where it forms dense mats, up to 10 cm thick. It stays sterile and propagates only in a vegetative manner. Frost resistance of Lower Silesia populations is higher than reported so far; fern may winter and rebuild the population after frost reaching 22oC. Size of the populations is changeable during the vegetation season. A. filiculoides occurs in water habitats and plant communities in which it substitutes Lemna minor.

  5. Lipid Yield and Composition of Azolla filiculoides and the Implications for Biodiesel Production

    OpenAIRE

    Brouwer, Paul; Werf, van der, W.; Schluepmann, Henriette; Reichart, Gert Jan; Nierop, Klaas G J

    2016-01-01

    The aquatic fern Azolla is one of the fastest-growing nitrogen-fixing plants on Earth and therefore considered as a potential source of biomass for bioenergy production. The lipid fraction from Azolla filiculoides was analyzed to investigate whether it suited biodiesel production. Since the productivity of Azolla is further increased at higher CO2 concentrations, A. filiculoides biomass was produced at 800 ppm CO2 mimicking a cultivation system utilizing CO2 waste from industry. The harvested...

  6. Response of growth and antioxidant enzymes in Azolla plants (Azolla pinnata and Azolla filiculoides) exposed to UV-B.

    Science.gov (United States)

    Masood, Amjad; Zeeshan, M; Abraham, G

    2008-06-01

    Effect of ultravilolet-B (0.4 Wm(-2)) irradiation on growth, flavonoid content, lipid peroxidation, proline accumulation and activities of superoxide dismutase and peroxidase was comparatively analysed in Azolla pinnata and Azolla filiculoides. Growth measured as increment in dry weight reduced considerably due to all UV-B treatments. However, the reduction was found to be severe in A. filiculoides as compared to A. pinnata. The level of UV-absorbing compound flavonoids increased significantly in A. pinnata plants whereas only a slight increase in the flavonoid content was observed in A. filiculoides. UV-B exposure led to enhanced production of malondialdehyde (MDA) and electrolyte leakage in A. filiculoides than A. pinnata. Proline accumulation also showed a similar trend. Marked differences in the activity of antioxidant enzymes such as superoxide dismutase (SOD) and peroxidase (POD) was noticed in both the plants exposed to UV-B. Our comparative studies indicate A. pinnata to be better tolerant to UV-B as compared with A. filiculoides which appears to be sensitive. PMID:18637563

  7. Lipid yield and composition of Azolla filiculoides and the implications for biodiesel production

    NARCIS (Netherlands)

    Brouwer, Paul; van der Werf, Adrie; Schluepmann, Henriette; Reichart, Gert Jan; Nierop, Klaas G J

    2016-01-01

    The aquatic fern Azolla is one of the fastest-growing nitrogen-fixing plants on Earth and therefore considered as a potential source of biomass for bioenergy production. The lipid fraction from Azolla filiculoides was analyzed to investigate whether it suited biodiesel production. Since the producti

  8. Lipid Yield and Composition of Azolla filiculoides and the Implications for Biodiesel Production

    NARCIS (Netherlands)

    Brouwer, Paul; Werf, van der Adrie; Schluepmann, Henriette; Reichart, Gert Jan; Nierop, Klaas G.J.

    2016-01-01

    The aquatic fern Azolla is one of the fastest-growing nitrogen-fixing plants on Earth and therefore considered as a potential source of biomass for bioenergy production. The lipid fraction from Azolla filiculoides was analyzed to investigate whether it suited biodiesel production. Since the produ

  9. Azolla filiculoides Nitrogenase Activity Decrease Induced by Inoculation with Chlamydomonas sp. †

    Science.gov (United States)

    Habte, Mitiku

    1986-01-01

    Experiments were conducted to determine the influence of Chlamydomonas sp. on nitrogen fixation (C2H2 → C2H4) in Azolla filiculoides and on the nitrogen fixation and growth of free-living Anabaena azollae 2B organisms. Inoculation of azolla medium with Chlamydomonas sp. was associated with decreased nitrogenase activity in A. filiculoides and with increases in the density of a fungal population identified as Acremonium sp. Subsequent inoculation of azolla medium with this fungus was also accompanied by a significant decrease in nitrogenase activity of A. filiculoides. However, the extent of depression of nitrogenase activity was significantly higher when azolla medium was inoculated with Chlamydomonas sp. than when it was inoculated with Acremonium sp. Inoculation of nitrogen-free Stanier medium with either Acremonium sp. or Chlamydomonas sp. did not adversely affect the growth or nitrogenase activity of free-living A. azollae. Decreased nitrogenase activity in A. filiculoides is apparently related to the adverse influence of the green alga and the fungus on the macrosymbiont. The mechanisms that might be involved are discussed. PMID:16347211

  10. Effects of the naturally-occurring contaminant microcystins on the Azolla filiculoides-Anabaena azollae symbiosis.

    Science.gov (United States)

    Pereira, A L; Monteiro, B; Azevedo, J; Campos, A; Osório, H; Vasconcelos, V

    2015-08-01

    Harmful algal blooms (HABs) contaminate aquatic ecosystems and are responsible for animal poisoning worldwide. We conducted a toxicity test with the aquatic fern and the biofertilizer, Azolla filiculoides. The sporophytes were exposed to three concentrations (0.01, 0.1 and 1μgmL(-1)) of a microcystin (MC) cyanobacterial crude extract and purified MC-LR. The growth of A. filiculoides decreased only at 1μgmL(-1) crude extract concentration while with MC-LR it decreased at all the tested concentrations, indicating that the presence of other compounds in the crude extract altered toxicity and stimulated the fern growth at lower concentrations (0.01 and 0.1μgmL(-1)). Both phycoerythrocyanin and allophycocyanin levels decreased in all the concentrations of crude extract and MC-LR. The phycocyanin had a marked increase at 0.1μgmL(-1) crude extract concentration and a marked decrease at 1μgmL(-1) MC-LR concentration. These changes in the phycobiliprotein content indicate a shift in the antenna pigments of the cyanobionts of A. filiculoides. The changes in two oxidative stress enzymes, glutathione reductase for the crude extract assay and glutathione peroxidase for MC-LR assay, points towards the induction of stress defense responses. The low bioconcentration factor in both crude extract and MC-LR treatments can suggest the low uptake of microcystins, and indicates that the aquatic fern can be used as a biofertilizer and as animal feed but is not suitable for MC phytoremediation. PMID:25890050

  11. Effects of the naturally-occurring contaminant microcystins on the Azolla filiculoides-Anabaena azollae symbiosis.

    Science.gov (United States)

    Pereira, A L; Monteiro, B; Azevedo, J; Campos, A; Osório, H; Vasconcelos, V

    2015-08-01

    Harmful algal blooms (HABs) contaminate aquatic ecosystems and are responsible for animal poisoning worldwide. We conducted a toxicity test with the aquatic fern and the biofertilizer, Azolla filiculoides. The sporophytes were exposed to three concentrations (0.01, 0.1 and 1μgmL(-1)) of a microcystin (MC) cyanobacterial crude extract and purified MC-LR. The growth of A. filiculoides decreased only at 1μgmL(-1) crude extract concentration while with MC-LR it decreased at all the tested concentrations, indicating that the presence of other compounds in the crude extract altered toxicity and stimulated the fern growth at lower concentrations (0.01 and 0.1μgmL(-1)). Both phycoerythrocyanin and allophycocyanin levels decreased in all the concentrations of crude extract and MC-LR. The phycocyanin had a marked increase at 0.1μgmL(-1) crude extract concentration and a marked decrease at 1μgmL(-1) MC-LR concentration. These changes in the phycobiliprotein content indicate a shift in the antenna pigments of the cyanobionts of A. filiculoides. The changes in two oxidative stress enzymes, glutathione reductase for the crude extract assay and glutathione peroxidase for MC-LR assay, points towards the induction of stress defense responses. The low bioconcentration factor in both crude extract and MC-LR treatments can suggest the low uptake of microcystins, and indicates that the aquatic fern can be used as a biofertilizer and as animal feed but is not suitable for MC phytoremediation.

  12. The invasion of Doñana National Park (SW Spain) by the mosquito fern (Azolla filiculoides Lam).

    OpenAIRE

    García Murillo, Pablo; Fernández Zamudio, María del Rocío; Cirujano Bracamonte, Santos; Sousa Martín, Arturo; Espinar, Juan Manuel

    2007-01-01

    In 2001, Azolla filiculoides Lam., a floating pteridophyte native to the New World, was found in Do˜nana National Park (SW Spain), an European protected area well known for its high value from a conservation point of view and for its ecological vulnerability. Until that time, there had been no exotic aquatic macrophyte observations in the National Park. Since then, surfaces covered by Azolla filiculoides have increased explosively forming thick floating mats which eliminate submersed...

  13. Arsenic accumulation by the aquatic fern Azolla: comparison of arsenate uptake, speciation and efflux by A. caroliniana and A. filiculoides.

    Science.gov (United States)

    Zhang, Xin; Lin, Ai-Jun; Zhao, Fang-Jie; Xu, Guo-Zhong; Duan, Gui-Lan; Zhu, Yong-Guan

    2008-12-01

    This study investigates As accumulation and tolerance of the aquatic fern Azolla. Fifty strains of Azolla showed a large variation in As accumulation. The highest- and lowest-accumulating ferns among the 50 strains were chosen for further investigations. Azolla caroliniana accumulated two times more As than Azolla filiculoides owing to a higher influx velocity for arsenate. A. filiculoides was more resistant to external arsenate due to a lower uptake. Both strains showed a similar degree of tolerance to internal As. Arsenate and arsenite were the dominant As species in both Azolla strains, with methylated As species accounting for Azolla in paddy fields to reduce As transfer from soil and water to rice should be further evaluated. PMID:18457908

  14. Phytodegradation potential of bisphenolA from aqueous solution by Azolla Filiculoides

    OpenAIRE

    Zazouli, Mohammad Ali; Mahdavi, Yousef; Bazrafshan, Edris; Balarak, Davoud

    2014-01-01

    Many organic hazardous pollutants such as bisphenolA (BPA) which are toxic and not easily biodegradable can concerns for environmental pollution worldwide. The objective of this study was to examine whether Azolla Filiculoides is able to remove BPA from aqueous solutions. In this study, the Azolla with different biomass (0.3, 0.6, 0.9, 1.2 g) has been cultured in solution that was contained 5, 10, 25 and 50 ppm BPA. Samples were collected every 2 days from all of containers. The analytical de...

  15. Arsenic accumulation by the aquatic fern Azolla: Comparison of arsenate uptake, speciation and efflux by A. caroliniana and A. filiculoides

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Xin [State Key Lab of Urban and ONAL Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Beijing 100085 (China); Lin Aijun [Department of Environmental Science and Engineering, Beijing University of Chemical Technology, Beijing 100029 (China); Zhao Fangjie [Soil Science Department, Rothamsted Research, Hertfordshire AL5 2JQ (United Kingdom); Xu Guozhong [Agricultural Ecology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013 (China); Duan Guilan [State Key Lab of Urban and ONAL Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Beijing 100085 (China); Zhu Yongguan [State Key Lab of Urban and ONAL Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Beijing 100085 (China); Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361003 (China)], E-mail: ygzhu@rcees.ac.cn

    2008-12-15

    This study investigates As accumulation and tolerance of the aquatic fern Azolla. Fifty strains of Azolla showed a large variation in As accumulation. The highest- and lowest-accumulating ferns among the 50 strains were chosen for further investigations. Azolla caroliniana accumulated two times more As than Azolla filiculoides owing to a higher influx velocity for arsenate. A. filiculoides was more resistant to external arsenate due to a lower uptake. Both strains showed a similar degree of tolerance to internal As. Arsenate and arsenite were the dominant As species in both Azolla strains, with methlyated As species accounting for <5% of the total As. A. filiculoides had a higher proportion of arsenite than A. caroliniana. Both strains effluxed more arsenate than arsenite, and the amount of As efflux was proportional to the amount of As accumulation. The potential of growing Azolla in paddy fields to reduce As transfer from soil and water to rice should be further evaluated. - Arsenic accumulation and efflux differ between strains of the aquatic fern Azolla.

  16. Phytodegradation potential of bisphenolA from aqueous solution by Azolla Filiculoides.

    Science.gov (United States)

    Zazouli, Mohammad Ali; Mahdavi, Yousef; Bazrafshan, Edris; Balarak, Davoud

    2014-01-01

    Many organic hazardous pollutants such as bisphenolA (BPA) which are toxic and not easily biodegradable can concerns for environmental pollution worldwide. The objective of this study was to examine whether Azolla Filiculoides is able to remove BPA from aqueous solutions. In this study, the Azolla with different biomass (0.3, 0.6, 0.9, 1.2 g) has been cultured in solution that was contained 5, 10, 25 and 50 ppm BPA. Samples were collected every 2 days from all of containers. The analytical determination of BPA was performed by using of DR4000 uv-visible at λmax = 276 nm. The results indicated that Azolla has high ability to remove BPA from aqueous solutions. The BPA removal was 60-90%. The removal efficiency is increasing with decreasing of BPA concentration and increasing of biomass amount and vice versa. The removal efficiency was more than 90% when BPA concentration was 5 ppm and amount of biomass was 0.9gr. It is concluded that Azolla able remove BPA by Phytodegradation from the aqueous solutions. Since conventional methods of BPA removal need to high cost and energy, phytoremediation by Azolla as a natural treatment system can decrease those issues and it can be a useful and beneficial method to removal of BPA. PMID:24693863

  17. Biotechnological potential of Azolla filiculoides for biosorption of Cs and Sr: Application of micro-PIXE for measurement of biosorption.

    Science.gov (United States)

    Ghorbanzadeh Mashkani, Saeid; Tajer Mohammad Ghazvini, Parisa

    2009-03-01

    The presence of Cs and Sr in culture medium of Azolla filiculoides caused about 27.4% and 46.3% inhibition of biomass growth, respectively, in comparison to A. filiculoides control weight which had not metals. Biosorption batch experiments were conducted to determine the Cs and Sr binding ability of native biomass and chemically modified biosorbents derived from Azolla namely ferrocyanide Azolla sorbents type 1 and type 2 (FAS1 and FAS2) and hydrogen peroxide Azolla sorbent (HAS). The best Cs and Sr removal results were obtained when A. filiculoides was treated by 2M MgCl(2) and 30ml H(2)O(2) 8mM at pH 7 for 12h and it was then washed by NaOH solution at pH 10.5 for 6h. Pretreatment of Azolla have been suggested to modify the surface characteristics which could improve biosorption process. The binding of Cs and Sr on the cell wall of Azolla was studied with micro-PIXE and FT-IR. PMID:19054668

  18. Phytoremediation of petroleum hydrocarbons by using a freshwater fern species Azolla filiculoides Lam.

    Science.gov (United States)

    Kösesakal, Taylan; Ünal, Muammer; Kulen, Oktay; Memon, Abdülrezzak; Yüksel, Bayram

    2016-01-01

    In this study, the phytoremediation capacity of Azolla filiculoides Lam. for the water resources contaminated with petroleum hydrocarbons was investigated. The plants were grown in nitrogen-free Hoagland nutrient solution containing 0.005%, 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, and 0.5% crude oil under greenhouse conditions for 15 days. Although the growth rate of the plants were not negatively influenced by the presence of crude oil in the media for the concentration of 0.005% and 0.01% v/v, a gradual impeding effect of crude oil in the growth media has been observed at concentrations 0.05-0.1%. More than 0.1% crude oil in the growth medium ostensibly retarded the growth. For example, 0.2% oil in the media reduced growth approximately 50% relative to the control, and the presence of crude oil at concentrations 0.3% or more were lethal. The data about the percentage of plant growth, fresh weight increase and root growth clearly indicated that the tolerance level of A. filiculoides plants to crude oil ranges between 0.1% and 0.2%. In comparison to control samples, the biodegradation rate of total aliphatic and aromatic (phenathrene) hydrocarbons at 0.05-0.2% oil concentrations, was 94-73% and 81-77%, respectively. On the other hand, in case of further increases in oil concentration in media, i.e.; 0.3-0.5%, the biodegradation rate was still higher in the experimental samples, respectively 71-63% and 75-71%. The high biodegradation rates of petroleum hydrocarbons in the experimental samples suggested that A. filiculoides plants could be a promising candidate to be used for the phytoremediation of low crude oil contaminated precious freshwater resources. PMID:26588199

  19. Linking azolla filiculoides invasion to increased winter temperatures in the doñana marshland (Sw spain)

    OpenAIRE

    Espinar, José L.; Díaz-Delgado, Ricardo; Bravo, Miguel A.; Vilà, Montserrat

    2015-01-01

    © 2015 The Author(s). Unravelling how a multiplicity of global change factors might influence the expansion of alien plants is a major goal of invasion biology. We explored the association of climatic conditions (i.e. annual rainfall and average minimum temperature during the coldest months) and water quality (orthophosphate, nitrate concentrations and electrical conductivity), with blooms of the invasive fern Azolla filiculoides Lam. in the Doñana marshland (SW Spain), one of the most extens...

  20. Report on the introduction experiment of Azolla filiculoides Lamk%细绿萍引种试验报告

    Institute of Scientific and Technical Information of China (English)

    崔国文; 陈雅君; 王明君; 殷秀杰; 黄庆峰

    2011-01-01

    详细叙述了细绿萍(Azolla filiculoides Lamk)从1979年4月引入黑龙江省以后至2009年的引种试验和生产试验推广全过程,特别对近10年间进行的春季扩繁速度和繁殖系数的品种比较以及水深度和施肥量对产草量影响等内容进行了细致研究.结果表明,细绿萍具有较强的耐低温能力,其最适宜生长繁殖温度为20℃左右;在相同条件下,细绿萍在单位面积的平均总产量和繁殖系数极显著高于绿萍;施磷肥可以显著提高细绿萍的产草量,适宜的施肥量为0.42kg·m-2;萍池水深度对细绿萍的生长速度有直接影响,在北方春季和初夏,较浅的水深度(10 cm)更有力于细绿萍的生长和繁殖.%The introduction of testing and promotion of the entire process of production test about the Azolla (Azolla filiculoides Lamk) introduced from April 1979 to 2009 in Heilongjiang Province was fully described. Especially in recent 10 years, the propagation speed in spring and propagation coefficient of variety comparatively as well as that the water depth and fertilizer effects on yield was thorough studied. The preliminary results were as follows: the azolla had strong cold tolerance, whose most proper growth temperature was about 20 ℃. Under the same condition, the average output per unit area and the propagation coefficient of the azolla, compared with the green duckweed, concentrated separately. P fertilizer could significantly improve the azolla produce in quantity, appropriate fertilizer to 0.42 kg· m-2. Water depth had a direct impact on the growth rate of small azolla. In the northern spring and early summer,shallow water depth (10 cm) was more effective in small azolla growth and reproduction.

  1. Comunidades de bacterias y protozoos asociados a la rizosfera de Azolla filiculoides, Lemma gibba y Ricciocarpos natans.

    OpenAIRE

    Quisehuatl Tepexicuapan, Eliceo

    2013-01-01

    Las raíces de las plantas exudan compuestos que promueven la proliferación de microorganismos, fenómeno conocido como efecto rizosfera. Este efecto ha sido ampliamente estudiado en raíces de plantas terrestres; sin embargo, poco se conoce sobre la influencia de las raíces de plantas acuáticas en las comunidades microbianas. Por ello se analizaron los géneros bacterianos cultivables y de protozoos presentes en la rizosfera de Azolla filiculoides, Lemna gibba y Ricciocarpos natans recolectadas ...

  2. Bioaccumulation of selected heavy metals by the water fern, Azolla filiculoides Lam. in a wetland ecosystem affected by sewage, mine and industrial pollution

    Energy Technology Data Exchange (ETDEWEB)

    Wet, L.P.D. de; Schoonbee, H.J.; Pretorius, J.; Bezuidenhout, L.M. (Rand Afrikaans University, Johannesburg (South Africa). Depts. of Zoology and Botany, Research Unit for Aquatic and Terrestrial Ecosystems)

    1990-10-01

    The bio-accumulation of the heavy metals, Fe, Cu, Ni, Pb, Zn, Mn and Cr by the water fern, Azolla filiculoides Lam. in a wetland ecosystem polluted by effluents from sewage works, mines and industries was investigated. Results showed that the different metals can be accumulated by the water fern at concentration levels not necessarily related to their actual concentrations in the aquatic environment, as measured in this case, in the bottom sediments. 45 refs., 1 fig., 3 tabs.

  3. Biochemical and growth performance of the aquatic macrophyte Azolla filiculoides to sub-chronic exposure to cylindrospermopsin.

    Science.gov (United States)

    Santos, Catarina; Azevedo, Joana; Campos, Alexandre; Vasconcelos, Vitor; Pereira, Ana L

    2015-11-01

    Physiological and biochemical effects of cylindrospermopsin (CYN), a cyanobacterial toxin that inhibits protein synthesis and released during a harmful cyanobacterial bloom, has been overlooked in plants. Therefore, at the present research, the toxic effects (physiological and biochemical) of a crude extract containing CYN were assessed in the aquatic fern Azolla filiculoides exposed to three concentrations (0.05, 0.5 and 5 μg CYN mL(-1)). At 5 μg CYN mL(-1), fern growth rate has showed a drastic decrease (0.001 g g(-1) day(-1)) corresponding to a 99.8% inhibition, but at the concentrations of 0.05 and 0.5 μg CYN mL(-1) the growth rate was similar to the control plants. Growth rate also indicated a IC50 of 2.9 μg CYN mL(-1). Those data point to the presence of other compounds in the crude extract may stimulate the fern growth and/or the fern is tolerant to CYN. Chlorophyll (a and b), carotenoids and protein content as well as the activities of glutathione reductase (GR) and glutathione-S-transferase (GST) has increased at 5 μg CYN mL(-1) which may indicate that photosynthesis and protein synthesis are not affected by CYN and the probable activation of defense and detoxifying mechanisms to overcome the effects induced by the presence of CYN. Low uptake of cylindrospermopsin (1.314 μg CYN g(-1) FW) and low bioconcentration factor (0.401) point towards to a safe use of A. filiculoides as biofertilizer and as food source, but also indicate that the fern is not suitable for CYN phytoremediation. PMID:26209169

  4. 细绿萍在动物生产中的应用%Application of Azolla Filiculoides Lamk in Animal Production

    Institute of Scientific and Technical Information of China (English)

    王国霞; 马立保; 柳英; 梅春升

    2006-01-01

    细绿萍(Azolla filiculoides Lamk)又名细满江红或蕨状满江红,属水生蕨类、满江红科、满江红属的藻萍共生植物,原产于美洲,1977年由中国科学院植物研究所从东德引入国内,因具有固氮能力,常作为饲料和肥料使用。

  5. Growth and feed utilization by juvenile grass carp (Ctenopharyngodon idella feed with Azolla filiculoides with low lipid diet/ Desempenho e conversão alimentar de juvenis de carpa capim (Ctenopharyngodon idella alimentadas com Azolla filiculoides e ração com baixo teor lipídico

    Directory of Open Access Journals (Sweden)

    Karina Dufech Esteves

    2008-08-01

    Full Text Available The total supply of fish available for human consumption will depend more of the expansion of low trophy level fish farming than of wild-caught fish. Aquatic plants used at treatment of aquaculture wastewater have been showed like an alternative to exclusive diet feed. The aquatic fern, Azolla filiculoides is an example with high level of protein. Consequently, more research on feed requirements of herbivorous fish is required. The aim of this study was to evaluate the growth performance and feed efficiency of juvenile grass carp (Ctenopharyngodon idella fed with three different diets using low lipid feed and water fern (Azolla filiculoides. The experiment was carried out in nine (9 tanks of 300 L capacity with three fish per tank (initial body weight = 55,43g. Feeding rate was 2.5% Fish were fed on two times a day with three treatments (R= Diet/Azolla on dry weight R= 2.5; R = 5 and R= 10. The specific growth rate (SGR = 1.39%, daily growth gain (DGG = 1.06 g / day and were significantly (p A oferta de pescado para o consumo humano dependerá mais da expansão do cultivo de peixes que se alimentam dos primeiros níveis tróficos do que daqueles oriundos da pesca extrativa. Plantas aquáticas usadas em fitotratamento de efluentes de aquacultura vêm sendo referidas como uma alternativa alimentar ao uso exclusivo de rações. A Azolla filiculoides é uma pteridófita que apresenta alto nível protéico. Assim, pesquisas sobre as exigências nutricionais de espécies herbívoras são necessárias. O objetivo principal deste estudo foi avaliar o crescimento e eficiência alimentar de juvenis de carpa capim (Ctenopharyngodon idella alimentadas com três relações (R entre ração com baixo teor lipídico e a planta aquática in natura (Azolla filiculoides. O experimento foi conduzido em nove reservatórios com capacidade de 300L e três indivíduos por unidade (Peso Inicial = 53,43g. A taxa alimentar diária foi de 2,5%, dividida em duas refei

  6. Potential of the aquatic fern Azolla filiculoides in biodegradation of an azo dye: modeling of experimental results by artificial neural networks.

    Science.gov (United States)

    Khataee, A R; Movafeghi, A; Vafaei, F; Lisar, S Y Salehi; Zarei, M

    2013-01-01

    The potential of an aquatic fern, Azolla filiculoides, in phytoremediation of a mono azo dye solution, C.I. Acid Blue 92 (AB92), was studied. The effects of operational parameters such as reaction time, initial dye concentration, fern fresh weight, pH, temperature and reusability of the fern on biodegradation efficiency were investigated. The intermediate compounds produced by biodegradation process were analyzed using GC-MS analysis. An artificial neural network (ANN) model was developed to predict the biodegradation efficiency. The findings indicated that ANN provides reasonable predictive performance (R2 = 0.961). The effects of AB92 solutions (10 and 20 mg L(-1)) on growth, chlorophylls and carotenoids content, activity of antioxidant enzymes such as superoxide dismutase, peroxidase and catalase and formation of malondialdehyde were analyzed. AB92 generally showed inhibitory effects on the growth. Moreover, photosynthetic pigments in the fronds significantly decreased in the treatments. An increase was detected for lipid peroxidation and antioxidant enzymes activity, suggesting that AB92 caused reactive oxygen species production in Azolla fronds, which were scavenged by induced activities of antioxidant enzymes. PMID:23819271

  7. Potential of the aquatic fern Azolla filiculoides in biodegradation of an azo dye: modeling of experimental results by artificial neural networks.

    Science.gov (United States)

    Khataee, A R; Movafeghi, A; Vafaei, F; Lisar, S Y Salehi; Zarei, M

    2013-01-01

    The potential of an aquatic fern, Azolla filiculoides, in phytoremediation of a mono azo dye solution, C.I. Acid Blue 92 (AB92), was studied. The effects of operational parameters such as reaction time, initial dye concentration, fern fresh weight, pH, temperature and reusability of the fern on biodegradation efficiency were investigated. The intermediate compounds produced by biodegradation process were analyzed using GC-MS analysis. An artificial neural network (ANN) model was developed to predict the biodegradation efficiency. The findings indicated that ANN provides reasonable predictive performance (R2 = 0.961). The effects of AB92 solutions (10 and 20 mg L(-1)) on growth, chlorophylls and carotenoids content, activity of antioxidant enzymes such as superoxide dismutase, peroxidase and catalase and formation of malondialdehyde were analyzed. AB92 generally showed inhibitory effects on the growth. Moreover, photosynthetic pigments in the fronds significantly decreased in the treatments. An increase was detected for lipid peroxidation and antioxidant enzymes activity, suggesting that AB92 caused reactive oxygen species production in Azolla fronds, which were scavenged by induced activities of antioxidant enzymes.

  8. Stress responses of duckweed (Lemna minor L.) and water velvet (Azolla filiculoides Lam.) to anionic surfactant sodium-dodecyl-sulphate (SDS).

    Science.gov (United States)

    Forni, C; Braglia, R; Harren, F J M; Cristescu, S M

    2012-04-01

    Surfactants are used for several purposes and recently they have attracted the attention for their ability to modify the behavior of other preexistent or co-disposed contaminants, although their use or discharge in wastewaters can represent a real or potential risk for the environment. Lemna minor L. and Azolla filiculoides Lam. are floating aquatic macrophytes, very effective in accumulating several pollutants including sodium dodecyl sulphate (SDS). In this work we evaluated the effects of SDS on these species by determining the stress ethylene production via laser-based trace gas detection, and the activities of enzymes involved in stress response, such as guaiacol peroxidase (G-POD), phenylalanine ammonia-lyase (PAL) and polyphenol-oxidase (PPO). Phenolics content was also determined. The macrophytes were treated with different concentrations of SDS for one week. SDS affected duckweed enzymatic activities and phenol content. While in the fern phenolics amount, PAL, G-POD and PPO activities were not affected by SDS except for 100 ppm SDS, the only concentration that was taken up and not completely degraded. Stress ethylene production was induced only in the fern treated with 50 and 100 ppm SDS. PMID:22277247

  9. Temporal variation in the biomass and nutrient status of Azolla filiculoides Lam. (Salviniaceae in a small shallow dystrophic lake Variação temporal de biomassa e estado nutricional de Azolla filiculoides Lam (Salviniaceae em um pequeno lago raso distrófico

    Directory of Open Access Journals (Sweden)

    Claudio Rossano Trindade Trindade

    2011-12-01

    Full Text Available AIM: This study determined the temporal variation of the biomass and the concentrations of carbon, nitrogen and phosphorus in Azolla filiculoides Lam. in a small (0.5 ha shallow dystrophic lake located in the city of Rio Grande (Rio Grande do Sul State, Brazil. METHOD: Sampling was conducted monthly between November 2000 and October 2001. The macrophytes were collected randomly in three replicates with a circular collector 0.3 m in diameter and subsequently washed with tap water and oven-dried at 60 ºC for determination of the dry weight and the nutrient status (i.e., carbon, nitrogen and phosphorus. Primary productivity was estimated by the variation in biomass among successive samples. RESULTS: A. filiculoides was present in the lake throughout the year and occupied between 50 and 80% of the surface area. The biomass values ranged from 34.2 g DW.m-2, recorded in May (autumn, to 170.9 g DW.m-2 in January (summer. The highest rate of primary productivity was 3.3 g DW.m-2.d-1, observed in June. The concentrations of carbon, nitrogen and phosphorus in the plant ranged between 403 and 551 g.kg-1, 13.4 and 25.7 g.kg-1 and 0.5 and 1.9 g.kg-1, respectively. The water N:P ratio ranged between 19:1 and 368:1. CONCLUSION: The coverage of the surface of the lake by A. filiculoides throughout the study period and the nutritional status of the plant demonstrate the importance of the cycling of nutrients by macrophytes in this aquatic environment. The higher N:P ratio in the water column, compared with other neighboring environments without macrophytes, shows that the enrichment of the lake may result from the biological N-fixation activity produced by A. filiculoides.OBJETIVO: Este estudo determinou a variação temporal da biomassa e as concentrações de carbono, nitrogênio e fósforo de Azolla filiculoides Lam., em um pequeno lago raso distrófico situado no município do Rio Grande (Rio Grande do Sul, Brasil. MÉTODO: As amostragens foram realizadas

  10. Kinetic modeling and thermodynamic study to remove Pb(II), Cd(II), Ni(II) and Zn(II) from aqueous solution using dead and living Azolla filiculoides

    Energy Technology Data Exchange (ETDEWEB)

    Rakhshaee, Roohan [Department of Applied Chemistry, Faculty of Chemistry, Islamic Azad University, Rasht Branch, P.O. Box 41335-3516, Rasht (Iran, Islamic Republic of); Khosravi, Morteza [Department of Applied Chemistry, Faculty of Chemistry, Islamic Azad University, North Tehran Branch, P.O. Box 191367-4711, Tehran 191367-4711 (Iran, Islamic Republic of)]. E-mail: drm_khosravi@yahoo.com; Ganji, Masoud Taghi [Department of Applied Chemistry, Faculty of Chemistry, Islamic Azad University, North Tehran Branch, P.O. Box 191367-4711, Tehran 191367-4711 (Iran, Islamic Republic of)

    2006-06-30

    Dead Azolla filiculoides can remove Pb{sup 2+},Cd{sup 2+}, Ni{sup 2+} and Zn{sup 2+} corresponding to second-order kinetic model. The maximum adsorption capacity (Q {sub max}) to remove these metal ions by the alkali and CaCl{sub 2}/MgCl{sub 2}/NaCl (2:1:1, molar ratio) activated Azolla from 283 to 313 K was 1.431-1.272, 1.173-0.990, 1.365-1.198 and 1.291-0.981 mmol/g dry biomass, respectively. Q {sub max} to remove these heavy metals by the non-activated Azolla at the mentioned temperature range was obtained 1.131-0.977, 1.092-0.921, 1.212-0.931 and 1.103-0.923 mmol/g dry biomass, respectively. In order to remove these metal ions by the activated Azolla, the enthalpy change ({delta}H) was -4.403, -4.495, -4.557 and -4.365 kcal/mol and the entropy change ({delta}S) was 2.290, 1.268, 1.745 and 1.006 cal/mol K, respectively. While, to remove these metal ions by the non-activated Azolla, {delta}H was -3.685, -3.766, -3.967 and -3.731 kcal/mol and {delta}S was 2.440, 1.265, 1.036 and 0.933 cal/mol K, respectively. On the other hand, the living Azolla removed these heavy metals corresponding to first-order kinetic model. It was also shown that pH, temperature and photoperiod were effective both on the rate of Azolla growth and the rate of heavy metals uptake during 10 days. It was appeared the use of Ca(NO{sub 3}){sub 2} increased both Azolla growth rate and the rate of heavy metals uptake while the using KNO{sub 3} although increased Azolla growth rate but decreased the rate of heavy metals uptake.

  11. 新疆引种蕨状满江红的生态适应性及生物学功能初探%Pilot study of ecological adaptability and biological function of Azolla filiculoides introduced in Xinjiang

    Institute of Scientific and Technical Information of China (English)

    姚晓玲; 姜彦成; 王德萍; 黄典典; 宋娅丽

    2003-01-01

    结合新疆的环境特点,对蕨状满江红(Azolla filiculoides Lam.)进行了一系列引种适应性试验和生物学功能测定.结果表明:蕨状满江红在新疆有很强的适应性,在5~40℃均能生长,其生长最适温度为25~30℃,且耐低温;在2~8 g/L的盐溶液及pH 4~10范围内也能生长,并且可降低水体的矿化度及pH值; 具有净化养殖水体和很强的富钾能力,植株钾含量最高可达干重的9.66%;水利用率高,并能有效减少养殖水体表面的蒸发,有保水作用.在新疆的河、湖和水库适量养殖蕨状满江红,可实现其生态与经济价值.

  12. Immunocytochemical localization of nitrogenase in bacteria symbiotically associated with Azolla spp.

    OpenAIRE

    Lindblad, P; Bergman, B.; Nierzwicki-Bauer, S A

    1991-01-01

    In situ immunogold labeling and transmission electron microscopy were used to detect nitrogenase in bacteria (bactobionts) symbiotically associated with leaf cavities of Azolla caroliniana and Azolla filiculoides. In A. caroliniana, the Fe protein of the nitrogenase complex was detected in a subset of the distinct bactobiont types present in leaf cavities of all ages. Similar results were obtained for the bactobionts of A. filiculoides with antisera against both the Fe and MoFe subunits of ni...

  13. Die beiden Azolla-arten des Niederländischen Pleistozäns

    NARCIS (Netherlands)

    Florschütz, F.

    1938-01-01

    Die erste Mitteilung über die Möglichkeit des Vorkommens fossiler Azolla im niederländischen Boden rührt von J. LORIé her, der im Jahre 1905 bei der Beschreibung von Bohrproben die Entdeckung von Makrosporangien von Azolla filiculoides Lam. in einer dünnen Torfschicht unter Vogelenzang erwähnte (Lit

  14. Azolla domestication towards a biobased economy?

    Science.gov (United States)

    Brouwer, Paul; Bräutigam, Andrea; Külahoglu, Canan; Tazelaar, Anne O E; Kurz, Samantha; Nierop, Klaas G J; van der Werf, Adrie; Weber, Andreas P M; Schluepmann, Henriette

    2014-05-01

    Due to its phenomenal growth requiring neither nitrogen fertilizer nor arable land and its biomass composition, the mosquito fern Azolla is a candidate crop to yield food, fuels and chemicals sustainably. To advance Azolla domestication, we research its dissemination, storage and transcriptome. Methods for dissemination, cross-fertilization and cryopreservation of the symbiosis Azolla filiculoides-Nostoc azollae are tested based on the fern spores. To study molecular processes in Azolla including spore induction, a database of 37 649 unigenes from RNAseq of microsporocarps, megasporocarps and sporophytes was assembled, then validated. Spores obtained year-round germinated in vitro within 26 d. In vitro fertilization rates reached 25%. Cryopreservation permitted storage for at least 7 months. The unigene database entirely covered central metabolism and to a large degree covered cellular processes and regulatory networks. Analysis of genes engaged in transition to sexual reproduction revealed a FLOWERING LOCUS T-like protein in ferns with special features induced in sporulating Azolla fronds. Although domestication of a fern-cyanobacteria symbiosis may seem a daunting task, we conclude that the time is ripe and that results generated will serve to more widely access biochemicals in fern biomass for a biobased economy. PMID:24494738

  15. Preliminary Screening of Azolla Strains for Tolerance to Ammonuim Ion

    OpenAIRE

    KITOH, Shunji; SHIOMI, Nobuyuki

    1995-01-01

    Azolla plants (117 strains from 7 species) were screened for tolerance to ammonium ion on the basis of their growth on the medium with or without ammonium. A. nilotica was the most sensitive species of all, followed by A. rubra whereas A. pinnata var. pinnata was the most tolerant one. From A. mexicana, A. caroliniana, A. microphylla and A. pinnata var. pinnata, 12 tolerant strains were selected. There was no tolerant strain in A. filiculoides as far as tested.

  16. Application of Azolla for 2, 4, 6-Trichlorophenol (TCP) Removal from Aqueous Solutions

    OpenAIRE

    Mohammad Ali Zazouli; Davoud Balarak; Yusef Mahdavi

    2013-01-01

    Background & Aims of the Study: The 2, 4, 6-Trichlorophenol (TCP) is a phenolic compound which it can produce adverse effects on human and environment. Therefore, the removal of these compounds is necessary. The aim of this study is the investigation of TCP removal by using Azolla filiculoides biomass. Materials & Methods: The Azolla biomass was dried in the sunlight, and then it was crushed and sieved to particle sizes in range of 1-2 mm. Next treated with 0.1M HCl for a period of 5h....

  17. Application of Azolla for 2, 4, 6-Trichlorophenol (TCP Removal from Aqueous Solutions

    Directory of Open Access Journals (Sweden)

    Mohammad Ali Zazouli

    2013-11-01

    Full Text Available Background & Aims of the Study: The 2, 4, 6-Trichlorophenol (TCP is a phenolic compound which it can produce adverse effects on human and environment. Therefore, the removal of these compounds is necessary. The aim of this study is the investigation of TCP removal by using Azolla filiculoides biomass. Materials & Methods: The Azolla biomass was dried in the sunlight, and then it was crushed and sieved to particle sizes in range of 1-2 mm. Next treated with 0.1M HCl for a period of 5h. The Azolla was washed with distilled water and it was used as adsorbent. The effect of operating parameters such as pH, contact time, TCP concentration and adsorbent dose on the TCP removal efficiency was investigated. The residues concentration of TCP was measured by spectrophotometer in λ max of 296 nm. Results: In optimum condition (pH 3, contact time 120 min , adsorbent dose 10 gr/l and TCP concentration 10 ppm, Azolla was able to remove 95% of TCP from aqueous solutions. The equilibrium data follows the Langmuir isotherm and the proper kinetic model is pseudo-second model. Conclusions: Adsorption process by Azolla filiculoides is an efficient method for removal of 2, 4, 6-Trichlorophenol from aqueous solutions.

  18. Use of Azolla as a growing medium component in the nursery production of olive trees

    OpenAIRE

    Petruccelli, Raffaella; Carlozzi, Pietro; Padovani, Giulia; Bartolini, Giorgio; Briccoli Bati, Caterina; Vignozzi, Nadia

    2015-01-01

    Peat is used in olive-tree nurseries as a component in commercial growing media. Environmental and economic pressures are questioning the use of peat as a component of substrates. In order to remain competitive and to satisfy environmental concerns, it is necessary to identify and evaluate possible alternatives to peat. This study involves the use of Azolla filiculoides, as a growing media component in olive nurseries (Olea europaea L.) for containerized plants. Four treatments were establish...

  19. Comparison of biomass productivity and nitrogen fixing potential of Azolla SPP

    Energy Technology Data Exchange (ETDEWEB)

    Arora, A.; Singh, P.K. [Indian Agricultural Research Inst., New Delhi (India)

    2003-03-01

    Study was conducted on six different Azolla species, available in the germplasm collection of NCCUBGA, IARI, New Delhi namely A. filiculoides, A. mexicana, A. microphylla, A. pinnata, A. rubra and A. caroliniana in a polyhouse to assess their growth potential by determining their maximal biomass productivity, doubling time and relative growth rates. Their nitrogen fixing potential was assessed by acetylene reduction assay. Among them Azolla microphylla gave highest biomass production and relative growth rate followed by Azolla caroliniana. Both these had high nitrogenase activity also. Peak nitrogenase activity of these strains was found on 14th day of growth and it declined on further incubation. Azolla microphylla and Azolla rubra were more tolerant to salinity than others. On the other hand Azolla pinnata, which is endemic species found in India, exhibited low biomass production, relative growth rate and lower nitrogenase activity compared to other species. It was unable to sustain growth in saline medium. Under polyhouse conditions, A. microphylla was found to perform better than other cultures in terms of biomass productivity, N fixing ability and salt tolerance. Hence it is taken up for mass production.(author)

  20. Localization and toxic effects of cadmium, copper, and uranium in Azolla

    Energy Technology Data Exchange (ETDEWEB)

    Sela, M.; Tel-Or, E.; Fritz, E.; Huttermann, A.

    1988-09-01

    The storage and distribution of copper, cadmium, and uranium and their effects on ionic contents in roots and shoots of Azolla filiculoides has been studied by x-ray microanalysis. The relative content of copper was eightfold higher in the root than in the shoot, suggesting low mobility of this metal in Azolla plant. Cadmium relative content in the shoot was similar to its content in the root, hence its mobility was relatively high. The absence of significant uranium quantities in the shoot and its relative high content in the root suggest the immobility of this metal from Azolla root. Cadmium formed precipitates with phosphate and calcium in xylem cells of the shoot bundle and caused a two- to threefold increase in the content of phosphate in the root. Uranium in roots and cadmium in shoots were associated with calcium. All three treatments caused losses of potassium, chloride, and magnesium from Azolla roots. Accumulation of heavy metals in Azolla and their mobility from the root to the shoot can be correlated with damage caused by the loss of essential nutrients.

  1. Nitrogen fixation by the Azolla-Anabaena azollae symbiosis

    International Nuclear Information System (INIS)

    A concise outline is presented on the main characteristics of the Azolla association in relation to tropical wetland rice cultivation and the nitrogen economy of paddy soils. Due to the presence of a nitrogen fixing cyanobiont occurring in a special leaf cavity of the Azolla leaf, the water fern Azolla can grow in a nitrogen-deficient environment and is able to contribute considerably to the nitrogen status of the soil. An experimental set-up is presented for how the nitrogen-fixing capacity of Azolla plants can be measured in the field by means of the acetylene reduction assay using a rather simple glass vessel. A comparison was made between 15N2 fixation by Azolla and acetylene reduction of Azolla plants under identical conditions

  2. Toxicity and biosorption of metals by saccharomyces cerevisiae, amorphotheca resinae and azolla filiculoides

    OpenAIRE

    Fogarty, Robert V.

    1998-01-01

    The value of H+ efflux in assessing and understanding metal interactions with Saccharomyces cerevisiae was investigated for its potential use as a rapid means of toxicity assessment for a range of metals Toxicity decreased in the order Cu2+ > Cd2+ > Pb2+ > Co2+ > Sr2+. Toxic effects can be alleviated by external Ca2+. The effect of Cu2+ and Co2+ on S cerevisiae growth, and the intracellular localisation of Cu2+, were studied in order to gain a better understanding of their toxicity. S cer...

  3. Cartografía de la superficie invadida por azolla filiculolides en el parque nacional de doñana mediante imágenes landsat.

    OpenAIRE

    Aragonés, David; Fernández-Zamudio, R.; Bustamante, Javier; García-Murillo, P.; Cirujano, Santos

    2009-01-01

    Azolla filiculoides es un helecho acuático invasor, que fue detectado por primera vez en la marisma Doñana en el año 2000. Aparece como cubierta dominante o por debajo de helófitos. En las zonas invadidas termina cubriendo totalmente la lámina de agua, modificando las características reflectivas de la superficie. La biología de esta especie (escaso tamaño y hábito flotante) junto con las características propias de la marisma (gran extensión y dificultad de acceso) no facilitan el seguimiento ...

  4. Sugar supported H/sub 2/ production and C/sub 2/H/sub 2/ reduction by the cyanobiont Anabaena azollae

    Energy Technology Data Exchange (ETDEWEB)

    Rozen, A.; Tel-Or, E.

    1986-01-01

    Sugar supported activities of H/sub 2/ production and C/sub 2/H/sub 2/ reduction were characterized in axenic cell cultures of the cyanobiont Anabaena azollae isolated from the water fern Azolla filiculoides. Fructose was found to be the favoured substrate, enhancing activities in both the light and the dark even at relatively low concentrations of 0.5-1.0 mM. Higher concentrations of sucrose, (10-20mM) also supported H/sub 2/ production and C/sub 2/H/sub 2/ reduction, while glucose was less effective. Levels of H/sub 2/ production were always lower than those of C/sub 2/H/sub 2/ reduction. 13 references.

  5. Azolla-Anabaena's behaviour in urban wastewater and artificial media--influence of combined nitrogen.

    Science.gov (United States)

    Costa, M L; Santos, M C R; Carrapiço, F; Pereira, A L

    2009-08-01

    The results of using the nitrogen fixing symbiotic system Azolla-Anabaena to improve the quality of treated urban wastewater, particularly on what concerns phosphorus removal efficiencies (40-65%), obtained in continuous assays performed during the past few years and presented earlier, were very promising. Nevertheless, the presence of combined nitrogen in some wastewaters can compromise the treatment efficiency. The main goal of this work was to compare plants behaviour in wastewater and in mineral media with and without added nitrogen. Azolla filiculoides's specific growth rates in wastewater and in mineral media without added nitrogen or with low nitrate concentration were very similar (0.122 d(-1)-0.126 d(-1)), but decreased in the presence of ammonium (0.100 d(-1)). The orthophosphate removal rate coefficients were similar in all the growth media (0.210 d(-1)-0.232 d(-1)), but ammonium removal rate coefficient in wastewater was higher (0.117 d(-1)) than in mineral medium using that source of nitrogen (0.077 d(-1)). The ammonium present in wastewater, despite its high concentration (34 mg NL(-1)), didn't seem to inhibit growth and nitrogen fixation, however, in mineral media, ammonium (40 mg NL(-1)) was found to induce, respectively, 18% and 46% of inhibition. PMID:19559459

  6. Mecanismos de tolerancia del simbiosistema Azolla-Anabaena azollae ante arsénico y cobre.

    OpenAIRE

    Sánchez Viveros, Gabriela

    2012-01-01

    Esta investigación evaluó algunos mecanismos de tolerancia del simbiosistema Azolla-Anabaena azollae ante agua contaminada con arsénico (As+5) y/o cobre (Cu2+). Para lo anterior, se plantearon seis fases experimentales: 1) identificar molecularmente a nivel de especie a diez colectas de Azolla, 2) evaluar la capacidad de acumulación de As+5 y los efectos tóxicos del metaloide en el simbiosistema Azolla-Anabaena azollae, 3) determinar la influencia del As+5 en la concentración de nueve element...

  7. Research on some functions of Azolla in CELSS system

    Science.gov (United States)

    Liu, Xiaofeng; Min, Chen; Xia-shi, Liu; Chungchu, Liu

    This article detailed the possibility of using Azolla in CELSS system, the characters of Azolla; the experiments on using Azolla as O 2-releasing plant to provide O 2 for human in airtight chamber; using Azolla as an important biological part for urine solution purification was also introduced.

  8. Azolla domestication towards a biobased economy?

    NARCIS (Netherlands)

    Brouwer, Paul; Bräutigam, A.; Külahoglu, C.; Tazelaar, A.O.E.; Kurz, S.; Nierop, K.G.J.; van der Werf, A.; Weber, A.P.M.; Schlupmann, Henriette

    2014-01-01

    Due to its phenomenal growth requiring neither nitrogen fertilizer nor arable land and its biomass composition, the mosquito fern Azolla is a candidate crop to yield food, fuels and chemicals sustainably. To advance Azolla domestication, we research its dissemination, storage and transcriptome. Meth

  9. Hydrogen uptake by Azolla-Anabaena

    International Nuclear Information System (INIS)

    The hydrogen uptake in the Azolla-Anabaena system is studied. Tritium is used as tracer. Plants are incubated under different atmosphere composition: a) Air + 3H2; b) Air + CO2 + 3H2 + CO; c) Air + 3H2 + CO; d) Air + CO2 + 3H2 + CO to study the pathway of absorbed hydrogen in the Azolla - Anabaena system. Azolla-Anabaena showed greater hydrogen uptake under argonium atmosphere than under air. Carbon monoxide decreased hydrogen uptake. There are evidences of recycling of the hydrogen evolved through notrogenease. (Author)

  10. Cytokinin-induced promotion of root meristem size in the fern Azolla supports a shoot-like origin of euphyllophyte roots.

    Science.gov (United States)

    de Vries, Jan; Fischer, Angela Melanie; Roettger, Mayo; Rommel, Sophie; Schluepmann, Henriette; Bräutigam, Andrea; Carlsbecker, Annelie; Gould, Sven Bernhard

    2016-01-01

    The phytohormones cytokinin and auxin orchestrate the root meristem development in angiosperms by determining embryonic bipolarity. Ferns, having the most basal euphyllophyte root, form neither bipolar embryos nor permanent embryonic primary roots but rather an adventitious root system. This raises the questions of how auxin and cytokinin govern fern root system architecture and whether this can tell us something about the origin of that root. Using Azolla filiculoides, we characterized the influence of IAA and zeatin on adventitious fern root meristems and vasculature by Nomarski microscopy. Simultaneously, RNAseq analyses, yielding 36,091 contigs, were used to uncover how the phytohormones affect root tip gene expression. We show that auxin restricts Azolla root meristem development, while cytokinin promotes it; it is the opposite effect of what is observed in Arabidopsis. Global gene expression profiling uncovered 145 genes significantly regulated by cytokinin or auxin, including cell wall modulators, cell division regulators and lateral root formation coordinators. Our data illuminate both evolution and development of fern roots. Promotion of meristem size through cytokinin supports the idea that root meristems of euphyllophytes evolved from shoot meristems. The foundation of these roots was laid in a postembryonically branching shoot system. PMID:26358624

  11. Phytoremediation potential of aquatic macrophyte, Azolla.

    Science.gov (United States)

    Sood, Anjuli; Uniyal, Perm L; Prasanna, Radha; Ahluwalia, Amrik S

    2012-03-01

    Aquatic macrophytes play an important role in the structural and functional aspects of aquatic ecosystems by altering water movement regimes, providing shelter to fish and aquatic invertebrates, serving as a food source, and altering water quality by regulating oxygen balance, nutrient cycles, and accumulating heavy metals. The ability to hyperaccumulate heavy metals makes them interesting research candidates, especially for the treatment of industrial effluents and sewage waste water. The use of aquatic macrophytes, such as Azolla with hyper accumulating ability is known to be an environmentally friendly option to restore polluted aquatic resources. The present review highlights the phytoaccumulation potential of macrophytes with emphasis on utilization of Azolla as a promising candidate for phytoremediation. The impact of uptake of heavy metals on morphology and metabolic processes of Azolla has also been discussed for a better understanding and utilization of this symbiotic association in the field of phytoremediation. PMID:22396093

  12. Azolla-Anabaena relationship. XIII. Fixation of (/sup 13/N)N/sub 2/. [Azolla caroliniana; Anabaena azollae

    Energy Technology Data Exchange (ETDEWEB)

    Meeks, J.C.; Steinberg, N.A.; Enderlin, C.S.; Joseph, C.M.; Peters, G.A.

    1987-07-01

    The major radioactive products of the fixation of (/sup 13/N)N/sub 2/ by Azolla caroliniana willd.-Anabaena azollae Stras. were ammonium, glutamine, and glutamate, plus a small amount of alanine. Ammonium accounted for 70 and 32% of the total radioactivity recovered after fixation for 1 and 10 minutes, respectively. The presence of a substantial pool of (/sup 13/N)N/sub 2/-derived /sup 13/NH/sub 4//sup +/ after long incubation periods was attributed to the spatial separation between the site of N/sub 2/-fixation (Anabaena) and a second, major site of assimilation (Azolla). Initially, glutamine was the most highly radioactive organic product formed from (/sup 13/N)N/sub 2/, but after 10 minutes of fixation glutamate had 1.5 times more radiolabel than glutamine. These kinetics of radiolabeling, along with the effects of inhibitors of glutamine synthetase and glutamate synthase on assimilation of exogenous and (/sup 13/N)N/sub 2/-derived /sup 13/NH/sub 4//sup +/, indicate that ammonium assimilation occurred by the glutamate synthase cycle and that glutamate dehydrogenase played little or no role in the synthesis of glutamate by Azolla-Azabaena.

  13. Effects of Atmospheric NO2 on Azolla-Anabaena Symbiosis.

    OpenAIRE

    Hur, Jae-Seoun; Wellburn, Alan R.

    1994-01-01

    Cultures of the water fern Azolla pinnata R, Br. exposed for 1 week to atmospheric NO2 (50, 100 or 200 nl l-1) induced additional levels of nitrate reductase (NaR) protein and nitrite reductase (NiR) activity. At low concentrations of NO2 (50 nl l-1), nitrate derived from NO2 provides an alternative N source for Azolla but does not affect rates of acetylene reduction. However, the symbiotic relationship between Azolla and its endosymbiont, Anabaena azollae is only affected adversely by high c...

  14. Heavy metal pollutant tolerance of Azolla pinnata

    Energy Technology Data Exchange (ETDEWEB)

    Sarkar, A.; Jana, S.

    1986-01-01

    The effects of Hg, As, Pb, Cu, Cd, and Cr (1,2 and 5 mg L/sup -1/ each) on Azolla pinnata R. Br. were analyzed. The treatments (2 and 5 mg L/sup -1/) of the heavy metal pollutants decreased Hill activity, chlorophyll, protein and dry wt, and increased tissue permeability over control values. The effects were most pronounced with the treatment of 5 mg L/sup -1/. The harmful effects of the metals were, in general, found by the treatments in the order: Cd > Hg > Cu > As > Pb > Cr. There was no significant change in these parameters at 1 mg L/sup -1/ of the metals over control. Thus Azolla pinnata shows tolerance to the heavy metals tested up to 1 mg L/sup -1/ each.

  15. The freshwater fern Azolla (Azollaceae) from Eocene Arctic and Nordic Sea sediments: New species and their stratigraphic distribution

    NARCIS (Netherlands)

    van der Burgh, J.; Collinson, M.E.; van Konijnenburg-van Cittert, J.H.A.; Barke, J.; Brinkhuis, H.

    2013-01-01

    Three new species of the freshwater fern Azolla are described from Eocene marine deposits of the Arctic and Nordic seas, bringing the total number of species now documented from these areas to five. Azolla arctica Collinson et al., Azolla jutlandica Collinson et al., Azolla nova sp. nov. and Azolla

  16. Pig-duck-fish-azolla integration in La Union, Philippines

    OpenAIRE

    Gavina, L.D.

    1994-01-01

    Three studies on pig-duck-fish-azolla integration were conducted simultaneously in La Union, Philippines. Growth performance of pigs and ducks as affected by different levels of azolla meal in their feed, and that of tilapia (Oreochromis niloticus) under varying stocking rates are presented and discussed.

  17. The omnipresent water fern Azolla caroliniana does not contain lignin

    NARCIS (Netherlands)

    Nierop, K.G.J.; Speelman, E.N.; Leeuw, J.W. de; Reichart, G.-J.

    2011-01-01

    Several studies have reported the presence of large amounts of lignin in ubiquitously occurring species of the freshwater fern Azolla. Molecular analysis using flash pyrolysis and thermally assisted hydrolysis and methylation shows, however, that neither the leaves nor the roots of Azolla contain li

  18. Biosorption of uranium by Azolla, SP, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, Ludmila C.; Alves, Eliakim G.; Marumo, Julio T., E-mail: lcvieira@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Ferreira, Rafael V. de P., E-mail: rafael@itatijuca.com [Itatijuca Biotech, Sao Paulo, SP (Brazil); Canevesi, Rafael L.S.; Silva, Edson A., E-mail: edson.silva2@unioeste.br [Universidade Estadual do Oeste Parana (UNIOESTE), Toledo, PR (Brazil)

    2015-07-01

    Radioactive liquid waste needs special attention and requires suitable treatment before deposition. Among the potential technologies under development for the treatment of liquid radioactive wastes the biosorption has been highlighted by being an efficient and low cost technique. Biosorption process involves the exchange of ions contained in the biomass matrix by others present in solution. There are many biomasses that could be applied in treatment of radioactive wastes, for example, agricultural residues and macrophyte. The aim of this study is evaluate the ability of the Azolla sp., a floating aquatic plant, to absorb uranium in solution. Azolla sp. is a macrophyte that has been used to treat effluents containing heavy metals. The biosorption capacity of uranium by Azolla sp. was experimentally determined and modeled by isotherms. Experiments were performed to determine metal uptake, and then the solutions were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES). The isotherms applied to model the data was Langmuir, Freundlich, Sips Toth, Redlich Peternson, Two-Site-Langmuir, Radke Prausnitz to develop a technique for the treatment of radioactive liquid waste generated at the Nuclear and Energy Research Institute (IPEN-CNEN/SP), Brazil. (author)

  19. Stratigraphy and paleoenvironment of the Danish Eocene Azolla event

    Science.gov (United States)

    Heilmann-Clausen, Claus; Beyer, Claus; Snowball, Ian

    2010-05-01

    Spores (massulae and megaspores) of the freshwater fern Azolla are recorded in several Danish Eocene outcrops and boreholes. The Azolla-bearing interval is 0.5 - ca. 3 m thick and occurs within the L2 Bed, a unit in the lower part of the hemipelagic, bathyal Lillebælt Clay Formation deposited in the central and eastern parts of the North Sea Basin. Intervals of organic-rich clay, usually including two distinctive, black sapropels, are present in the lower part of Bed L2, indicating a generally reduced oxygen content in the bottom waters during this time, with at least two episodes of severe, basinwide stagnation. The oxygen-deficit points to reduced circulation and/or enhanced marine productivity in the North Sea Basin. Azolla occurs in the upper part of this mainly organic-rich interval. The frequency of Azolla spores relative to marine dinoflagellate cysts fluctuates within the interval. The Azolla interval has previously been correlated to levels near the Ypresian/Lutetian transition in Belgium, based on dinoflagellate stratigraphy. Calibration of a new magnetostratigraphic study of the lower Lillebælt Clay with the dinoflagellate biostratigraphy suggests that Bed L2 spans the upper part of Chron 22r, C22n and lower part of C21r. The Azolla pulse spans the upper part of C22n and lowermost part of C21r. The combined bio-magnetostratigraphy from Denmark allows a detailed comparison with published data from the northern part of the Norwegian-Greenland Sea (ODP Hole 913B). The correlation confirms earlier assumptions, which were based on biostratigraphy alone, that the marine Azolla pulse in the two areas, and therefore probably over the whole Norwegian-Greenland Sea - North Sea region, is of the same age. An ongoing palynological study of the L2 Bed has so far revealed no indication for freshwater episodes or brackish waters in the basin during the Azolla pulse, except perhaps for Azolla itself. It is, therefore, suggested that the Azolla spores were transported

  20. Azolla as a nitrogen fertilizer in sustainable rice production

    International Nuclear Information System (INIS)

    Alternatives to the use of commercial N fertilizers must therefore be sought if we are to sustain crop production. Plant species such as Azolla which in symbiosis with the blue-green alga Anabena azollae are capable of converting atmospheric N2 into usable forms through the microbial process of biological N2 fixation. Such nitrogen fixing systems offer an attractive and ecologically sound means of reducing external inputs of chemical N fertilizers in cropping systems. Azolla is a free-floating water fern widely distributed in aquatic habitats of the tropics and sub-tropics. Because of its aquatic nature, Azolla is of particular value to flooded rice. Several methods have been used to estimate biological N2 fixation of Azolla. In a Co-ordinated Research Programme on Azolla executed by the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, quantitative integrated values for biological N2 fixation by Azolla have been obtained through use of the 15N isotope. Azolla can derive as much as 70% or more of its N from N2 fixation, yielding 22-24 kg N/ha in about one month. Differences however in N2 fixation are common depending on the Azolla species or strain and the environmental conditions. In this research programme, we also used 15N as a tracer to assess the N recovery by rice from Azolla and N balance in the rice cropping system. Incorporation of 15N labelled Azolla into soil gave an 15N recovery by rice of 40-50%. On an overall basis under a wide range of environmental conditions in six countries, the percentage 15N recovery by rice as well as the rice yield increases from Azolla application were similar to those obtained from urea. The general conclusion from this programme is that Azolla is as good as urea as a source of N for rice. There is no more important crop in the world today than rice. It occupies 90% of the area under cereals in the Asia and Pacific region. Research aimed at developing improved integrated fertilizer management practices

  1. Establishment and management of Azolla in rice fields

    International Nuclear Information System (INIS)

    This paper summarizes available information on the growth and maintenance of Azolla in rice fields, and is based primarily upon reports from rice producing countries in Asia. Since methods for large scale production of sporocarps are not available, Azolla inoculum must be produced vegetatively. For experimental purposes this can be accomplished in galvanized iron trays with a 2 cm layer of soil and 5 cm of water. The only necessary addition is concentrated super phosphate at a rate of 1.5g/m2 every 5 days. Phosphorous is the major nutrient limiting Azolla growth under field conditions, and P deficient Azolla is more susceptible to damage by high light intensity. Heavier inoculums favour establishment and growth of Azolla. To favour initial growth, the inoculum can be limited to a smaller area by floating bamboo or twisted rice straw ropes. Azolla is susceptible to several pests. Common pesticides used in rice culture also control most Azolla pests, but biological control methods may also be effective. Suggestions are made on problems which could be profitably investigated using 14C, 15N and 32P. (author)

  2. Aquatic microphylla Azolla: a perspective paradigm for sustainable agriculture, environment and global climate change.

    Science.gov (United States)

    Kollah, Bharati; Patra, Ashok Kumar; Mohanty, Santosh Ranjan

    2016-03-01

    This review addresses the perspectives of Azolla as a multifaceted aquatic resource to ensure ecosystem sustainability. Nitrogen fixing potential of cyanobacterial symbiont varies between 30 and 60 kg N ha(-1) which designates Azolla as an important biological N source for agriculture and animal industry. Azolla exhibits high bioremediation potential for Cd, Cr, Cu, and Zn. Azolla mitigates greenhouse gas emission from agriculture. In flooded rice ecosystem, Azolla dual cropping decreased CH4 emission by 40 % than did urea alone and also stimulated CH4 oxidation. This review highlighted integrated approach using Azolla that offers enormous public health, environmental, and cost benefits. PMID:26697861

  3. Aquatic microphylla Azolla: a perspective paradigm for sustainable agriculture, environment and global climate change.

    Science.gov (United States)

    Kollah, Bharati; Patra, Ashok Kumar; Mohanty, Santosh Ranjan

    2016-03-01

    This review addresses the perspectives of Azolla as a multifaceted aquatic resource to ensure ecosystem sustainability. Nitrogen fixing potential of cyanobacterial symbiont varies between 30 and 60 kg N ha(-1) which designates Azolla as an important biological N source for agriculture and animal industry. Azolla exhibits high bioremediation potential for Cd, Cr, Cu, and Zn. Azolla mitigates greenhouse gas emission from agriculture. In flooded rice ecosystem, Azolla dual cropping decreased CH4 emission by 40 % than did urea alone and also stimulated CH4 oxidation. This review highlighted integrated approach using Azolla that offers enormous public health, environmental, and cost benefits.

  4. Azolla - A Model Organism for Plant Genomic Studies

    Institute of Scientific and Technical Information of China (English)

    Yin-Long Qiu; Jun Yu

    2003-01-01

    The aquatic ferns of the genus Azolla are nitrogen-fixing plants that have great potentials in agricultural production and environmental conservation. Azolla in many aspects is qualified to serve as a model organism for genomic studies because of its importance in agriculture, its unique position in plant evolution, its symbiotic relationship with the N2-fixing cyanobacterium, Anabaena azollae, and its moderate-sized genome. The goals of this genome project are not only to understand the biology of the Azolla genome to promote its applications in biological research and agriculture practice but also to gain critical insights about evolution of plant genomes. Together with the strategic and technical improvement as well as cost reduction of DNA sequencing, the deciphering of their genetic code is imminent.

  5. Polyamine-Induced Rapid Root Abscission in Azolla pinnata

    OpenAIRE

    Sushma Gurung; Cohen, Michael F.; Jon Fukuto; Hideo Yamasaki

    2012-01-01

    Floating ferns of the genus Azolla detach their roots under stress conditions, a unique adaptive response termed rapid root abscission. We found that Azolla pinnata plants exhibited dose-dependent rapid root abscission in response to the polyamines spermidine and spermine after a substantial time lag (>20 min). The duration of the time lag decreased in response to high pH and high temperature whereas high light intensity increased the time lag and markedly lowered the rate of abscission. The ...

  6. A Study on Azolla as a Nitrogen Source in Rice Farming

    OpenAIRE

    Gevrek, Mithat Nuri

    2000-01-01

    This study was conducted to determine the best combination of azolla ( Azolla anabaena) and N fertilizer under Menemen ecological conditions in 1996 and 1997. The A. mexicana genotype of azolla was brought from the International Rice Research Institute (IRRI) and adapted to the Aegean region of Turkey. The results of study showed that the combination azolla+N fertilizer yielded approximately 356 kg/da of rice under Menemen second crop conditions. It was also concluded that the use of azoll...

  7. Response of nitrogen-fixing water fern Azolla biofertilization to rice crop

    OpenAIRE

    Bhuvaneshwari, K.; Singh, Pawan Kumar

    2014-01-01

    The water fern Azolla harbors nitrogen-fixing cyanobacterium Anabaena azollae as symbiont in its dorsal leaves and is known as potent N2 fixer. Present investigation was carried out to study the influence of fresh Azolla when used as basal incorporation in soil and as dual cropped with rice variety Mahsoori separately and together with and without chemical nitrogen fertilizer in pots kept under net house conditions. Results showed that use of Azolla as basal or dual or basal plus dual influen...

  8. Population Dynamics of Biota on the Roots of Azolla microphylla Kaulfuss

    OpenAIRE

    NITA ETIKAWATI; JUTONO

    2000-01-01

    Azolla was a special fern that their associations with Anabaena azollae able to fix free nitrogen from air, to produce protein. Although by the ages, biota diversity those habits on the roots of Azolla increased and effected to protein concentration. The research was to find out population dynamics of biota on the roots of Azolla microphylla Kaulfuss and the growth peak. This study used Completely Randomized Design with 10 kinds of biota, i.e. bacteria, Fungi, Actinomycetes, Protozoa, Alga, C...

  9. Agricultural production - Phase 2. Indonesia. Rice - azolla - fish culture - use of nuclear technique

    International Nuclear Information System (INIS)

    The primary aim of the expert mission was to provide advice on the use of nuclear techniques to study rice-azolla-fish culture. Results of the work performed so far show that basal application of azolla gives similar or better yields of rice than basal application of urea. Fish productivity was also found to be significantly higher when azolla is present. 2 tabs

  10. Climatic conditions governing extensive Azolla bloom during the Middle Eocene

    Science.gov (United States)

    Dekker, Rolande; Speelman, Eveline N.; Barke, Judith; Konijnendijk, Tiuri; Sinninge Damste, Jaap S.; Reichart, Gert-Jan

    2010-05-01

    Enormous amounts of intact mega- and microspores from the free floating aquatic fern Azolla were found in sediments recovered during Integrated Ocean Drilling Program expedition 302, indicating that Azolla grew and reproduced in situ in the Eocene Arctic Ocean. In general, the Early/Middle Eocene is characterized by enhanced greenhouse conditions with elevated sea surface temperatures (SSTs) in the Arctic (~10°C), while tropical sea surface temperatures (SSTs) were only a little warmer than today (with a mean annual temperature (MAT) of 32-34 °C) (Pearson et al., 2007). The consequently reduced temperature gradient between the equator and the poles and the presence of freshwater at the North Pole as indicated by the presence of the freshwater fern Azolla (Brinkhuis et al., 2006) provide important boundary conditions for understanding the hydrological cycle and latent heat transport during this interval. Here we reconstruct variations in SST and mean annual air temperature using the TEX86 and MBT temperature proxies for the Azolla interval. Sediments from around the Arctic Basin have been analyzed, including samples from Alaska, the Mackenzie Basin, Greenland (IODP core 913b), and Denmark. Furthermore, a high resolution sea surface temperature record for the Azolla interval has been constructed from sediment samples from the Lomonosov Ridge, showing a cyclic signal. Model experiments have shown that the here confirmed low equator-to-pole temperature gradient modulated the hydrological cycle. Since the growth of Azolla is restricted to low salinity conditions, changes in the hydrological cycle are proposed to coincide with the cyclic occurrence of Azolla throughout the interval. To confirm the overlapping presence of high quantities of Azolla and increased precipitation, changes in the hydrogen cycle are reconstructed by creating a high resolution hydrogen isotope record throughout the interval. By performing compound specific analyses (δD) on terrestrial derived

  11. Cambios edáficos en las Marismas de Doñana y su relación con la invasión de Azolla filiculoide

    OpenAIRE

    Luis V. García; Gutiérrez González, Eduardo; Espinar, José L.; Cara García, Juan S.; Clemente Salas, Luis

    2007-01-01

    2 páginas, 7 referencias. En el Capítulo: Degradación de suelos y desertificación. Simposio celebrado en Fuerteventura, 16-20, septiembre, 2007. Los editores son del Departamento de Edafología y Geología, Universidad de La Laguna.

  12. VARIASI KOMBINASI TEPUNG LABU KUNING (Cucurbita moschata D.) DAN TEPUNG AZOLLA (Azolla pinnata R.br.) PADA KECERAHAN WARNA IKAN KOI (Cyprinus carpio L.)

    OpenAIRE

    Utami, Diah Ayu Tri

    2014-01-01

    Warna memegang peranan penting dalam menentukan kualitas ikan koi (Cyprinus carpio L.), bahkan dipandang sebagai komponen penting dalam proses seleksi ikan hias. Permasalahan yang sering timbul adalah memudarnya warna ikan bila dipelihara dalam jangka waktu yang lama. Penelitian ini menambahkan tepung labu kuning (Cucurbita moschata D.) dan tepung Azolla (Azolla pinnata R.Br.) ke dalam pakan buatan. Dengan penambahan tepung labu kuning dan tepung Azolla ini memungkinkan ikan memperoleh lebih ...

  13. Aquatic Fern (Azolla Sp.) Assisted Synthesis of Gold Nanoparticles

    Science.gov (United States)

    Jha, Anal K.; Prasad, K.

    2016-02-01

    Aquatic pteridophyte (Azolla sp.) was taken to assess its potential to synthesize the metal (Au) nanoparticles. The synthesized particles were characterized using X-ray, UV-visible, scanning and transmission electron microscopy analyses. Nanoparticles almost spherical in shape having the sizes of 5-17nm are found. UV-visible study revealed the surface plasmon resonance at 538nm. Responsible phytochemicals for the transformation were principally phenolics, tannins, anthraquinone glycosides and sugars present abundantly in the plant thereby bestowing it adaptive prodigality. Also, the use of Azolla sp. for the synthesis of gold nanoparticles offers the benefit of eco-friendliness.

  14. Breeding of backcrossed Azolla strain MH3-1 and its application prospect%回交萍3号的育种及其应用前景(综述)

    Institute of Scientific and Technical Information of China (English)

    唐龙飞; 刘中柱

    2004-01-01

    A back-crossing trial on Azolla that using hybrid Azolla strain Rongping 1 (A. filiculoides × A.microphylla) as male parent, A. microphylla as female parent was conducted, large amount of green seedlings and some of albino seedlings were acquired. Comparing with zymographic identification, morphological observation and physiological analysis, these seedlings were conformed as hybrid progenies. Further screening was conducted by indoor and outdoor trials on adverse condition-resistance and analysis of nutrient quality. A back-crossed strain with nice agronomic character was screened from numerous green seedlings of this hybrid combination, and named as MH3-1. The applying efficiency of MH3-1 in agricultural and husbandry production were introduced, its applying prospect in the future production of organic agriculture was analyzed.%以杂交满江红榕萍1号(细绿满江红×小叶满江红)为父本,以小叶满江红为母本进行满江红的回交试验,获得大量绿苗和少量白化苗.经同工酶谱鉴定比较、形态观察、生理生化分析确定为杂种后代.并经过室内外抗逆性筛选和品质分析,从该杂交组合后代中筛选出1株性状优良的回交苗,定名为回交萍3号(MH3-1).本文介绍了回交萍3号在农牧业生产上的应用效果,并分析了其在未来有机农业生产中的应用前景.

  15. Mosquito larval productivity in rice-fields infested with Azolla in Mvomero District, Tanzania.

    Science.gov (United States)

    Mwingira, V S; Mayala, B K; Senkoro, K P; Rumisha, S F; Shayo, E H; Mlozi, M R S; Mboera, L E G

    2009-01-01

    Azolla (Salviniales: Azollaceae) is known to reduce oviposition and adult emergence of a number of mosquito species. Several species of Azolla are reportedly indigenous to Tanzania. However, the potential of Azolla as a biocontrol agent against malaria mosquitoes has not been evaluated in the country. This cross-sectional study was carried out to assess mosquito larval productivity in irrigated rice-fields infested with Azolla in Mvomero District, Tanzania. A systematic larval sampling covering all open water bodies along designed transect was carried in rice-fields. Larval density was estimated by dipping water bodies with or without Azolla. The degree of Azolla coverage was categorized as 0%, 80%. Larvae densities were categorised as low ( or = 500/m2) productivity. A total of 120 water bodies were surveyed and 105 (87.5%) had Azolla microphyla and A. pinnata at varying degrees of coverage. Of the total 105 water bodies with Azolla, 80 (76.2%) had a green Azolla mat, and 25 (23.8%) a brown Azolla mat. Eighty-eight (73.3%) of the sites were infested with anophelines and 109 (90.8%) with culicine larvae. Seventy percent of all water bodies contained anophelines and culicines in sympatric breeding, while 20.8% and 3.3% had only culicines and anophelines, respectively. The majority (82%) of mosquito breeding sites were found in area with Azolla substrate. Mosquito larva productivity was low in sites with highest (>80%) Azolla coverage. Seventy-two (81.8%) of the anopheline and 90 (82.6%) culicine breeding sites were infested with Azolla. Water bodies infested with green Azolla were more productive than those covered by brown coloured Azolla substrates for both culicines (13%) and anophelines (8%). Of the 1537 field collected larvae that hatched to adult stage, 646 (42.03%) were Anopheles gambiae s.l., 42 (2.73%) were An. funestus and 769 (50.03%) were Culex quinquefasciatus. These findings suggest that the mosquito productivity is low when the Azolla coverage is high

  16. Pengaruh Waktu Aplikasi Dan Pemberian Berbagai Dosis Kompos Azolla (Azolla spp.) Terhadap Pertumbuhan Dan Produksi Tanaman Kailan (Brassica oleraceae Var. Acephala DC.)

    OpenAIRE

    Eko Andi Pasaribu

    2009-01-01

    Penelitian ini bertujuan untuk menguji pengaruh waktu aplikasi dan pemberian berbagai dosis kompos Azolla (Azolla spp.) terhadap pertumbuhan dan produksi tanaman kailan (Brasisca oleraceae Var. Acephala DC.). Penelitian dilaksanakan di lahan/areal percobaan Fakultas Pertanian Universitas Sumatera Utara Medan, dari bulan Juli 2008 sampai September 2008. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) faktorial dengan dua faktor perlakuan dan 3 ulangan. Fa...

  17. Microcosm investigation on phytoremediation of Cr using Azolla pinnata.

    Science.gov (United States)

    Rai, Prabhat Kumar

    2010-01-01

    The extent of Chromium (Cr) pollution in Singrauli industrial region, India was assessed and phytoremediation capacity of a small water fern, Azolla pinnata R.BR (Azollaceae) was observed to purify waters polluted by Cr under microcosm condition. Azolla pinnata endemic to India is a potential hyper-accumulator of heavy metals. During 13 days of the experiment the fern was grown in the aqueous medium containing Cr3+ and CrO4(2-) ions, each in a concentration 0.5, 1.0, and 3.0 mg L(-1). The presence of these ions caused a + 3.1 to -37.5% inhibition of Azolla pinnata growth in comparison to the control. After 13 days of the experiment, metal contents in the solution was decreased up to 70% (CrO4(2-) 3.0 mg L(-1) treatment) to 88% (CrO4(2-) 0.5 mg L(-1) treatment). In the Azolla pinnata tissues, the concentration of couple of the ionic forms of Cr under investigation ranged from 415 to 1095 mg kg(-1) dry mass (dm); the highest level being found for Cr (III) containing solution. PMID:20734631

  18. Tingkat Penggunaan Azolla Pinnata Pada Pakan Terhadap Pertumbuhan Ikan Nila (Oreochromis niloticus).

    OpenAIRE

    Sinaga, Daniel

    2015-01-01

    Nila fish is economic valuable fish, consumer demand increasing, but has not been followed by intensive cultivation and optimal. One of attempt to overcome these problems by substituting commercial feed with fish meat meal Azolla pinnata for growth nila fish. The tested fish are nila fish size 6 cm which maintained in an pain diameter 50 cm by 12 unit. With completely randomized design. With treatment commercial pakan 100% without added azolla flour, commercial pakan 94 % added 10 % azolla f...

  19. Mengukur Faktor Biokonsentrasi Azolla microphylla Dalam Menyerap Logam Berat Timbal (Pb)

    OpenAIRE

    Althair, Guntur

    2015-01-01

    Lead (Pb) in water was found in dissolved and suspended form. Lead toxicity to plants was relatively lower compared to other microscopic elements. This study aimed to determine the ability of Azolla microphylla absorb heavy metals at different concentrations of Pb and Pb heavy metal influence on the development of Azolla microphylla biomass. This study was conducted from February to March 2014 in the Laboratory of Manajemen Sumberdaya Perairan Universitas Sumatera Utara. Azolla microphyll...

  20. Micro-halocline enabled nutrient recycling may explain extreme Azolla event in the Eocene Arctic Ocean

    OpenAIRE

    Kempen, Monique M. L.; Smolders, Alfons J. P.; Lamers, Leon P. M.; Roelofs, Jan G. M.

    2012-01-01

    In order to understand the physicochemical mechanisms that could explain the massive growth of Azolla arctica in the Eocene Arctic Ocean, we carried out a laboratory experiment in which we studied the interacting effects of rain and wind on the development of salinity stratification, both in the presence and in the absence of a dense Azolla cover. Additionally, we carried out a mesocosm experiment to get a better understanding of the nutrient cycling within and beneath a dense Azolla cover in...

  1. GROWTH AND PHOTOSYNTHESIS OF THREE AZOLLA SPECIES IN RESPONSE TO IRRADIANCE

    OpenAIRE

    Daniel, J. N.; Bartholomew, D. P.

    1993-01-01

    Physiological studies of Azolla (azolla) species in relation to comparative responses to environment are limited. In this study, growth, carbon dioxide exchange rate (CER) and evapotranspiration (ET) were measured in Azolla caroliniana, A. microphylla and A. pinnata mats grown in a greenhouse at 100, 65, and 35% of ambient light levels. Although A. caroliniana had higher frond area index (FAI) and specific frond area, its CER per unit frond weight was significantly lower than A. microphylla a...

  2. Accumulation of mercury by Azolla and its effect on growth

    Energy Technology Data Exchange (ETDEWEB)

    Mishra, B.B.; Nanda, D.R.; Misra, B.N.

    1987-10-01

    Of the various industrial set-ups polluting the environment with mercury, chloralkali factories are the most important. The presence of mercury in the exhaust air has been reported, and the presence of a large amount of mercury has also been reported in the solid waste of chloralkali factories. The loss of mercury from the culture medium through microbial activities under laboratory conditions has been established. The present investigation deals with the effect of mercury contaminated solid wastes of chloralkali factories on the growth of Azolla (A water-weed) and loss of mercury from the medium under field conditions following its culture. It was noticed that the discharge of chloralkali greatly affected the local vegetation including cultivated cereals. An attempt was, therefore, made to reclaim the waste soil with Azolla.

  3. Coeval Eocene blooms of the freshwater fern Azolla in and around Arctic and Nordic seas

    NARCIS (Netherlands)

    Barke, J.; Burgh, A.H.P. van der; Konijnenburg-van Cittert, J.H.A. van; Collinson, M.E.; Pearce, M.A.; Bujak, J.; Heilman-Clausen, C.; Lotter, A.F.; Speelman, E.N.; Kempen, M.M.L. van; Reichart, G.-J.; Brinkhuis, H.

    2012-01-01

    For a short time interval (c. 1.2 Myr) during the early middle Eocene (~ 49 Myr), the central Arctic Ocean was episodically densely covered by the freshwater fern Azolla, implying sustained freshening of surface waters. Coeval Azolla fossils in neighboring Nordic seas were thought to have been sourc

  4. The optimum energy harvest efficiency of nitrogen fixing hydrophyte: Azolla pinnata

    Energy Technology Data Exchange (ETDEWEB)

    Tennakone, K. (Institute of Fundamental Studies, Kandy (LK) Ruhuna Univ., Matara (LK). Dept. of Physics); Punchihewa, S.; Jayasuriya, A.C. (Institute of Fundamental Studies, Kandy (LK))

    1989-01-01

    Azolla is a nitrogen fixing hydrophyte that can be cultivated in absence of nitrogenous fertilizer. It is found that when biomass is continuously harvested from a culture of Azolla, solar energy can be converted at an optimum efficiency of 1.1%. (author).

  5. PROTEIN FRACTIONATION AND IN VITRO DIGESTIBILITY OF AZOLLA IN RUMINANTS

    Directory of Open Access Journals (Sweden)

    S. PARASHURAMULU

    2013-05-01

    Full Text Available A study was undertaken to evaluate the nutritive value and digestibility of Azolla in ruminants by in vitro techniques. The crude protein, crude fibre and ether extract contents were at a level of 21.37%, 12.5% and 2.3%, respectively. The neutral and acid detergent fibre levels were about 35.4 and 23.9%, respectively. The average in vitro dry matter digestibility, in vitro organic matter digestibility and metabolozable energy contents were 79.5%, 63.8 mg/200mg and 7.36 MJ/kg DM (1759 kcal/kg, respectively. The various protein fractions A, B1, B2, B3 and C estimated by Cornell net crude protein solubility system were 18.22, 42.56, 15.15, 7.47 and 16.61% of total protein, respectively. The Azolla contained significantly higher B1 fraction followed by A, B2 and C and lowest fraction of C. Thus in view of above, present study indicated Azolla to be a good source protein supplement with 21.37% crude protein with highest B protein fractions, moderate source of energy (1759 kcal ME/kg, high dry matter and organic matter digestibilities and rich in trace minerals thus could be used as an alternate protein supplement or as supplementary protein supplement to ruminants.

  6. Orbitally-forced Azolla blooms and middle Eocene Arctic hydrology; clues from palynology

    Science.gov (United States)

    Barke, Judith; Abels, Hemmo A.; Sangiorgi, Francesca; Greenwood, David R.; Sweet, Arthur R.; Donders, Timme; Lotter, Andre F.; Reichart, Gert-Jan; Brinkhuis, Henk

    2010-05-01

    The presence of high abundances of the freshwater fern Azolla in the early Middle Eocene central Arctic Ocean sediments recovered from the Lomonosov Ridge during IODP Expedition 302, have been related to the presence of a substantial freshwater cap. Azolla massulae, belonging to the newly described Eocene species Azolla arctica Collinson et al., have been found over at least a ~4 m-thick interval. There are strong indications that Azolla has bloomed and reproduced in situ in the Arctic Ocean for several hundreds of thousands of years. Possible causes for the sudden demise of Azolla at ~48.1 Ma include salinity changes due to evolving oceanic connections or sea-level change. Distinct cyclic fluctuation in the Azolla massulae abundances have previously been related to orbitally forced climate changes. In this study, we evaluate the possible underlying forcing mechanisms for these freshwater cycles and for the eventual demise of Azolla in an integrated palynological and cyclostratigraphical approach. Our results show two clear periodicities of ~1.3 and ~0.7 m in all major aquatic and terrestrial palynomorph associations, which we can relate to obliquity (41 ka) and precession (~21 ka), respectively. Cycles in the abundances of Azolla, freshwater-tolerant dinoflagellate cysts, and swamp vegetation pollen show co-variability in the obliquity domain. Their strong correlation suggests periods of enhanced rainfall and runoff during Azolla blooms, possibly associated with increased summer season length and insolation during obliquity maxima. Cycles in the angiosperm pollen record are in anti-phase with the Azolla cycles. We interpret this pattern as edaphically drier conditions on land and reduced associated runoff during Azolla lows, possibly corresponding to obliquity minima. The precession signal is distinctly weaker than that for obliquity, and is mainly detectable in the cold-temperate Larix and bisaccate conifer pollen abundances, which is interpreted as a response to

  7. Determining biotoxins in continental waters colonised by Azolla; Determinacion de biotoxinas en aguas continentales colonizadas por Azolla

    Energy Technology Data Exchange (ETDEWEB)

    Prada A-Buylla, J.; Sanchez Crespo, R.; Verdigo Althofer, M. [Confederacion Hidrografica del Guadina. Ciudad Real (Spain)

    1999-07-01

    During the period 1993-95, the presence of Azolla ferns was observed in the air/water interface along several kilometres of the river Guadina in both Spain and Portugal. This fern is symbiotically associated with the cyano-bacteria Anabaena azollae that is capable of fixing atmospheric nitrogen, which can lead to vegetable growth and eutrophication. At the same time, the cyanobacteria may generate biotoxins, in which case a study is made of the hepatotoxins forming a family of 53 related cyclic peptides. Those consisting of seven aminoacids receive the name of microcystines, while formed by five aminoacids arc called nodulaincs. Chromatography was used to determine the presence of these peptides in the water from six sampling points. Microcystine-LR was found at concentrations of around 0.3 g/l at three points. (Author) 5 refs.

  8. Population Dynamics of Biota on the Roots of Azolla microphylla Kaulfuss

    Directory of Open Access Journals (Sweden)

    NITA ETIKAWATI

    2000-01-01

    Full Text Available Azolla was a special fern that their associations with Anabaena azollae able to fix free nitrogen from air, to produce protein. Although by the ages, biota diversity those habits on the roots of Azolla increased and effected to protein concentration. The research was to find out population dynamics of biota on the roots of Azolla microphylla Kaulfuss and the growth peak. This study used Completely Randomized Design with 10 kinds of biota, i.e. bacteria, Fungi, Actinomycetes, Protozoa, Alga, Crustacean, Rotifers, Coelenterate, Insect and Molluscs, and it was used 3 replications. Research was conducted within 4 weeks and the populations of biota were observed every week. Data were statistically analyzed using Analysis Variant and Duncan’s Multiple Range Test. The population dynamics of biota on the roots of Azolla microphylla Kaulfuss were influenced on its quantity and composition, and the growth peak is done in 2nd week.

  9. Study on O2-supplying characteristics of Azolla in Controlled Ecological Life Support System

    Science.gov (United States)

    Chen, Min; Deng, Sufang; Yang, Youquang; Huang, Yibing; Liu, Zhongzhu

    Azolla has high growth and propagation rate, strong photosynthetic O2-releasing ability and rich nutrient value. It is able to be used as salad-type vegetable, and can also be cultured on wet bed in multi-layer condition. Hence, it possesses a potential functioning as providing O2, fresh vegetable and absorbing CO2 for Controlled Ecological Life Support System in space. In this study, we try to make clear the O2-providing characteristics of Azolla in controlled close chamber under manned condition in order to lay a foundation for Azolla as a biological component in the next ground simulated experiment and space application. A closed test cham-ber of Controlled Ecological Life Support System and Azolla wet-culturing devices were built to measure the changes of atmospheric O2-CO2 concentration inside chamber under "Azolla-fish -men" coexisting condition. The results showed that, the amount of O2 consumption is 80.49 83.07 ml/h per kilogram fish, the amount of CO2 emissions is 70.49 73.56 ml/(kg • h); O2 consumption of trial volunteers is 19.71 L/h, the volume of respiration release CO2 18.90 L/h .Artificial light intensity of Azolla wet culture under 70009000 Lx, people respiration and Azolla photosynthesis complemented each other, the atmospheric O2-CO2 concentration inside chamber maintained equilibration. Elevated atmospheric CO2 concentrations in close chamber have obvious effects on enhancing Azolla net photosynthesis efficiency. This shows that Azolla has strong photosynthetic O2-releasing ability, which equilibrates the O2-CO2 concentration inside chamber in favor of human survival, and then verifies the prospect of Azolla in space application.

  10. The Eocene Arctic Azolla bloom: environmental conditions, productivity and carbon drawdown.

    Science.gov (United States)

    Speelman, E N; Van Kempen, M M L; Barke, J; Brinkhuis, H; Reichart, G J; Smolders, A J P; Roelofs, J G M; Sangiorgi, F; de Leeuw, J W; Lotter, A F; Sinninghe Damsté, J S

    2009-03-01

    Enormous quantities of the free-floating freshwater fern Azolla grew and reproduced in situ in the Arctic Ocean during the middle Eocene, as was demonstrated by microscopic analysis of microlaminated sediments recovered from the Lomonosov Ridge during Integrated Ocean Drilling Program (IODP) Expedition 302. The timing of the Azolla phase (approximately 48.5 Ma) coincides with the earliest signs of onset of the transition from a greenhouse towards the modern icehouse Earth. The sustained growth of Azolla, currently ranking among the fastest growing plants on Earth, in a major anoxic oceanic basin may have contributed to decreasing atmospheric pCO2 levels via burial of Azolla-derived organic matter. The consequences of these enormous Azolla blooms for regional and global nutrient and carbon cycles are still largely unknown. Cultivation experiments have been set up to investigate the influence of elevated pCO2 on Azolla growth, showing a marked increase in Azolla productivity under elevated (760 and 1910 ppm) pCO2 conditions. The combined results of organic carbon, sulphur, nitrogen content and 15N and 13C measurements of sediments from the Azolla interval illustrate the potential contribution of nitrogen fixation in a euxinic stratified Eocene Arctic. Flux calculations were used to quantitatively reconstruct the potential storage of carbon (0.9-3.5 10(18) gC) in the Arctic during the Azolla interval. It is estimated that storing 0.9 10(18) to 3.5 10(18) g carbon would result in a 55 to 470 ppm drawdown of pCO2 under Eocene conditions, indicating that the Arctic Azolla blooms may have had a significant effect on global atmospheric pCO2 levels through enhanced burial of organic matter. PMID:19323694

  11. Efficacy of oxygen-supplying capacity of Azolla in a controlled life support system

    Science.gov (United States)

    Chen, Min; Deng, Sufang; Yang, Youquan; Huang, Yibing; Liu, Chongchu

    2012-02-01

    Azolla shows high growth and propagation rates, strong photosynthetic O2-releasing ability and high nutritional value. It is suitable as a salad vegetable and can be cultured on a multi-layered wet bed. Hence, it possesses potential as a fresh vegetable, and to release O2 and absorb CO2 in a Controlled Ecological Life Support System in space. In this study, we investigated the O2-providing characteristics of Azolla in a closed chamber under manned, controlled conditions to lay a foundation for use of Azolla as a biological component in ground simulation experiments for space applications. A closed test chamber, representing a Controlled Ecological Life Support System including an Azolla wet-culture device, was built to measure the changes in atmospheric O2 and CO2 concentrations inside the chamber in the presence of coexisting Azolla, fish and men. The amount of O2 consumed by fish was 0.0805-0.0831 L kg-1 h-1 and the level of CO2 emission was 0.0705-0.0736 L kg-1 h-1; O2 consumption by the two trial volunteers was 19.71 L h-1 and the volume of respiration-released CO2 was 18.90 L h-1. Under 7000-8000 Lx artificial light and Azolla wet-culture conditions, human and fish respiration and Azolla photosynthesis were complementary, thus the atmospheric O2 and CO2 concentrations inside chamber were maintained in equilibrium. The increase in atmospheric CO2 concentration in the closed chamber enhanced the net photosynthesis efficiency of the Azolla colony. This study showed that Azolla has strong photosynthetic O2-releasing ability, which equilibrates the O2 and CO2 concentrations inside the chamber in favor of human survival and verifies the potential of Azolla for space applications.

  12. The potential of the fresh-water fern Azolla in aquatic farming systems

    Science.gov (United States)

    Bijl, Peter K.; Werf, vd, Adrie; Schluepmann, Henriette; Reichart, Gert-Jan; Brouwer, Paul; Nierop, Klaas G. J.; Hellgardt, Klaus; Brinkhuis, Henk

    2014-05-01

    With aquatic farming systems a new avenue in agriculture is explored, in which the competition with conventional arable land is avoided. The aquatic, ubiquitous, floating fern Azolla is not yet widely explored as potential crop in such farming systems, despite its high potential because it grows in many natural systems under low-light intensities, has an enormous annual yield, and has special biomass qualities for applications in food, feed and specialty chemical industries. But, what makes Azolla particularly interesting as cost-effective crop is its capability to take up atmospheric nitrogen through symbiosis with nitrogen-fixing bacteria Anabaena azollae. This makes Azolla independent of nitrogen fertilization. In order to explore the potential of Azolla as a crop for a suite of applications, we have assembled a team of expertise: AZOFAST, consisting of agricultural engineers, plant physiologists, chemical engineers and organic chemists. Our growth experiments reveal high annual production yields with constant harvest. We are developing a germination and spore collecting/preservation protocol as a first step to domestication. Finally we have explored the biomass quality of different species of extant Azolla. We performed organic chemical analyses on lipid and tannin extracts, and quantified yields of specific compounds within these fractions. In our presentation we will present some of our results to show the potential of Azolla as a new, sustainable aquatic crop serving all kinds of industrial streams from protein feed to platform chemicals.

  13. Micro-halocline enabled nutrient recycling may explain extreme Azolla event in the Eocene Arctic Ocean.

    Science.gov (United States)

    van Kempen, Monique M L; Smolders, Alfons J P; Lamers, Leon P M; Roelofs, Jan G M

    2012-01-01

    In order to understand the physicochemical mechanisms that could explain the massive growth of Azolla arctica in the Eocene Arctic Ocean, we carried out a laboratory experiment in which we studied the interacting effects of rain and wind on the development of salinity stratification, both in the presence and in the absence of a dense Azolla cover. Additionally, we carried out a mesocosm experiment to get a better understanding of the nutrient cycling within and beneath a dense Azolla cover in both freshwater and brackish water environments. Here we show that Azolla is able to create a windproof, small-scale salinity gradient in brackish waters, which allows for efficient recycling of nutrients. We suggest that this mechanism ensures the maintenance of a large standing biomass in which additional input of nutrients ultimately result in a further expansion of an Azolla cover. As such, it may not only explain the extent of the Azolla event during the Eocene, but also the absence of intact vegetative Azolla remains and the relatively low burial efficiency of organic carbon during this interval. PMID:23166833

  14. Micro-halocline enabled nutrient recycling may explain extreme Azolla event in the Eocene Arctic Ocean.

    Directory of Open Access Journals (Sweden)

    Monique M L van Kempen

    Full Text Available In order to understand the physicochemical mechanisms that could explain the massive growth of Azolla arctica in the Eocene Arctic Ocean, we carried out a laboratory experiment in which we studied the interacting effects of rain and wind on the development of salinity stratification, both in the presence and in the absence of a dense Azolla cover. Additionally, we carried out a mesocosm experiment to get a better understanding of the nutrient cycling within and beneath a dense Azolla cover in both freshwater and brackish water environments. Here we show that Azolla is able to create a windproof, small-scale salinity gradient in brackish waters, which allows for efficient recycling of nutrients. We suggest that this mechanism ensures the maintenance of a large standing biomass in which additional input of nutrients ultimately result in a further expansion of an Azolla cover. As such, it may not only explain the extent of the Azolla event during the Eocene, but also the absence of intact vegetative Azolla remains and the relatively low burial efficiency of organic carbon during this interval.

  15. Potential of Azolla as a bioferitizer for rice farming in Sri Lanka: Demonstrated by isotopic techniques

    International Nuclear Information System (INIS)

    Studies using stable, heavy isotope of nitrogen(N-15) were conducted to (1) estimate quantitatively nitrogen fixation by Azolla under rice field conditions and (2) evaluate the uptake of Azolla-N and urea-N by rice when incorporated into soil, as well as when using them in combination. While nitrogen fixation was estimated by the N-15 substrate labelling technique, uptake of N from labelled material was evaluated in polythene lined 1 square meter field microplots. Grain yield data was obtained from 4m into 5m yield plots having corresonding treatments. Farmers' experiments were also conducted in farmers' fields to examine the realization of the potentials demonstrated in research stations. Results showed that 55 to 66 per cent Azolla-N was derived from the atmosphere and 43 to 65 per cent Azolla-N was taken up by an associated rice crop. N-recovery from Azolla was better than from urea, particularly when it was incorporated at tillering. As Azolla cover left unincorporated among broadcast seeded rice enhanced the uptake of N from urea fertilizer. An integrated use of Azolla with urea has the potential to reduce the use of chemical fertilizer by 50 per cent, under farmer's field conditions (AU)

  16. Cultivation of irrigated-paddy rice with Azolla and Nila fish application

    OpenAIRE

    Kaimuddin; Ibrahim, Bachrul; Tangko, Lina

    2011-01-01

    This research aimed to find out effect of Azolla and Nila fish in cultivation of irrigated- paddy rice to increase rice production, and to find out reduction of inorganic fertilizer usage due to integration of Azolla and Nila fish. An experiment was carried out in Maros, South Sulawesi from December 2005 to March 2006. It was arranged in a split plot design. Results showed that Azolla???Nila integration in rice field increased grain by 17.05% (30.245 kg plot-1) and decreased we...

  17. INFLUENCE OF AZOLLA-ANABAENA SYMBIOSIS ON RICE (Oryza sativa L) CROP AS A NUTRITIONAL ALTERNATIVE

    OpenAIRE

    Castro, R; R. Novo; R. I. Castro

    2003-01-01

    Con el objetivo de estudiar la influencia de la asociación o incorporación de Azolla en el cultivo del arroz en condiciones controladas, se llevó a cabo un experimento durante el período comprendido entre mayo y octubre de 1997, usando un diseño completamente aleatorizado. Se evaluaron 16 tratamientos, productos de la combinación de cuatro niveles de nitrógeno (0, 40, 80, 120 kg N.ha-1) y cuatro sistemas de cultivo (arroz en monocultivo, arroz+Azolla incorporada, arroz+Azolla incorporada+Azol...

  18. Crowdfunding the Azolla fern genome project: a grassroots approach.

    Science.gov (United States)

    Li, Fay-Wei; Pryer, Kathleen M

    2014-01-01

    Much of science progresses within the tight boundaries of what is often seen as a "black box". Though familiar to funding agencies, researchers and the academic journals they publish in, it is an entity that outsiders rarely get to peek into. Crowdfunding is a novel means that allows the public to participate in, as well as to support and witness advancements in science. Here we describe our recent crowdfunding efforts to sequence the Azolla genome, a little fern with massive green potential. Crowdfunding is a worthy platform not only for obtaining seed money for exploratory research, but also for engaging directly with the general public as a rewarding form of outreach.

  19. Crowdfunding the Azolla fern genome project: a grassroots approach.

    Science.gov (United States)

    Li, Fay-Wei; Pryer, Kathleen M

    2014-01-01

    Much of science progresses within the tight boundaries of what is often seen as a "black box". Though familiar to funding agencies, researchers and the academic journals they publish in, it is an entity that outsiders rarely get to peek into. Crowdfunding is a novel means that allows the public to participate in, as well as to support and witness advancements in science. Here we describe our recent crowdfunding efforts to sequence the Azolla genome, a little fern with massive green potential. Crowdfunding is a worthy platform not only for obtaining seed money for exploratory research, but also for engaging directly with the general public as a rewarding form of outreach. PMID:25276348

  20. Pengaruh Pemberian Tepung Eceng Gondok (Eichornia grassipes) dan Paku Air (Azolla Pinnata) Fermentasi terhadap Performans Ayam Broiler (The Effect of Utilization of Fermented Water Hyacinth [Eichornia Grassipes] and Axis Water [Azolla Pinnata] with Asper

    OpenAIRE

    Saleh, Eniza; Rifai, Joharnomi; Sari, Enna

    2009-01-01

    Penelitian ini bertujuan untuk menguji pengaruh penggunaan tepung eceng gondok dan Azolla pinnata fermentasi terhadap konsumsi ransum, pertambahan bobot badan, dan konversi ransum. Metode penelitian ini memakai rancangan acak lengkap dengan 5 perlakuan, 4 ulangan dengan masing-masing plot terdiri dari 5 ekor ayam. Perlakuan R0 merupakan ransum kontrol (basal), R1 = pemberian 5% eceng gondok + 20% Azolla pinnata dalam ransum, R2 = Pemberian 10% eceng gondok + 15% Azolla pinnata dalam ransum, R...

  1. Effects of atmospheric SO[sub 2] on Azolla and Anabaena symbiosis

    Energy Technology Data Exchange (ETDEWEB)

    Hur, J.-S.; Wellburn, A.R. (Division of Biological Sciences, Institute of Environmental and Biological Sciences, Lancaster Univ., Lancaster (United Kingdom))

    1993-01-01

    The water fern Azolla pinnata R. Br. was fumigated for 1 week with either 25, 50 or 100 nl l[sup -1] SO[sub 2]. The symbiosis of Azolla with Anabaena azollae (spp.) was severely damaged by atmospheric SO[sub 2] even at the lowest concentration studied showing significant reductions in growth, reduction of C[sub 2]H[sub 2], NH[sub 3] assimilation, protein synthesis, and heterocyst development. These disturbances appear to be mainly responsible for the extreme sensitivity of this fern to atmospheric SO[sub 2]. Changes in violaxanthin/antheraxanthin and epoxylutein/lutein ratios also indicate that free radical products are induced by atmospheric SO[sub 2]. These results suggest that the Azolla-Anabeana symbiotic system is a very responsive and reliable lower plant model to study the detailed effects of total sulfur deposition upon the balances between various important plant metabolic processes.

  2. Polyamine-Induced Rapid Root Abscission in Azolla pinnata

    Directory of Open Access Journals (Sweden)

    Sushma Gurung

    2012-01-01

    Full Text Available Floating ferns of the genus Azolla detach their roots under stress conditions, a unique adaptive response termed rapid root abscission. We found that Azolla pinnata plants exhibited dose-dependent rapid root abscission in response to the polyamines spermidine and spermine after a substantial time lag (>20 min. The duration of the time lag decreased in response to high pH and high temperature whereas high light intensity increased the time lag and markedly lowered the rate of abscission. The oxidation products of polyamines, 1,3-diaminopropane, β-alanine and hydrogen peroxide all failed to initiate root abscission, and hydroxyethyl hydrazine, an inhibitor of polyamine oxidase, did not inhibit spermine-induced root abscission. Exposure of A. pinnata to the polyamines did not result in detectable release of NO and did not affect nitrite-dependent NO production. The finding of polyamine-induced rapid root abscission provides a facile assay for further study of the mode of action of polyamines in plant stress responses.

  3. The effects of SO sub 2 on Azolla - Anabaena symbiosis

    Energy Technology Data Exchange (ETDEWEB)

    Jaeseoun Hur; Wellburn, A.R. (Lancaster Univ. (United Kingdom))

    1991-05-01

    Cultures of Azolla pinnata containing Anabaena were investigated as a sensitive and reproducible bioindicator of air pollution. Three equal doses of SO{sub 2} (week*ppb: 1*100, 2*50, 4*25) were applied to Azolla cultures growing in nitrogen-free medium in a specially-designed exposure system. Exposure to high concentrations of SO{sub 2} showed highly significant reductions in growth of the fern, while nitrogen fixation and heterocyst development were severely damaged. This was associated with a reduction of protein content in the SO{sub 2}-exposed ferns and again more significant at higher SO{sub 2} levels. There was a variation in the absolute amount of the individual pigments between SO{sub 2} doses and/or treatments which was related to the physiological development of the ferns throughout the fumigations. Moreover, the ratio of violaxanthin to antheraxanthin in the 100 ppb SO{sub 2}-treated ferns was significantly higher than that in the clean air-grown ferns. The results clearly demonstrate that SO{sub 2} has adverse effects on the symbiosis and suggest that this fern is a promising bioindicator of air pollution and a very good model to investigate the inter-relationships between photosynthesis, nitrogen fixation and air pollution stress.

  4. Polyamine-Induced Rapid Root Abscission in Azolla pinnata.

    Science.gov (United States)

    Gurung, Sushma; Cohen, Michael F; Fukuto, Jon; Yamasaki, Hideo

    2012-01-01

    Floating ferns of the genus Azolla detach their roots under stress conditions, a unique adaptive response termed rapid root abscission. We found that Azolla pinnata plants exhibited dose-dependent rapid root abscission in response to the polyamines spermidine and spermine after a substantial time lag (>20 min). The duration of the time lag decreased in response to high pH and high temperature whereas high light intensity increased the time lag and markedly lowered the rate of abscission. The oxidation products of polyamines, 1,3-diaminopropane, β-alanine and hydrogen peroxide all failed to initiate root abscission, and hydroxyethyl hydrazine, an inhibitor of polyamine oxidase, did not inhibit spermine-induced root abscission. Exposure of A. pinnata to the polyamines did not result in detectable release of NO and did not affect nitrite-dependent NO production. The finding of polyamine-induced rapid root abscission provides a facile assay for further study of the mode of action of polyamines in plant stress responses. PMID:22997568

  5. Studies on Using Azolla for O2-Supplementation and Its Test

    Science.gov (United States)

    Liu, C.-C.; Liu, X.-S.; Chen, M.; Bian, Z.-L.

    Establishment of Controlling Ecological Life Safety System (CELSS) is a key technical part in the study on manned station. In order to meet the requirement of long-term out-earth man-carrying flight, it is necessary to build plant-based O2-supplying CELSS. It also possesses the possibility to contribute the realization of mankind dream to live in out-earth planets. Using the physiological reaction of organisms, this system settled the problem in food supply, O2 and water recycle, the regeneration and utilization of CO2 and life residues, thus guarantee the spacemen the health, safety and efficient work when they are far from earth and arrived the space where human is difficult to life in. Azolla as a plant that possesses high growing speed, short growing cycle, high photosynthesis and O2-releasing capacity, and can be artificially grow in multi-layer facilities, it is hopeful to be an important biological part in CELSS for it's the function in O2 production and to be a part of fresh vegetable as well as CO2 absorption. This paper described the study in Azolla as a plant for supplementary O2 supply in future space station flight. The "Azolla-dog" controlling tight system was firstly established in order to determine the regulation of O2-CO2 variation and balance using different weights of dogs with different Azolla growing areas. The further studies included the development of air-tight cabinets where "Azolla-human" airtight experiments were conducted, and the relationship between Azolla-growing area and O2 requirement by human was analyzed. Based on these works, the further experiment on supplementary O2-supply of Azolla-human system was conducted in demonstration cabin for environmental controlling and life security. The O2-supplying amount of Azolla was further gained, and it will provide the experimental basis to probe optimum condition to grow Azolla in space and human-machine combining experiment, also to lay a basis for Azolla as a biological part to enter

  6. Arctic Ocean circulation during the anoxic Eocene Azolla event

    Science.gov (United States)

    Speelman, Eveline; Sinninghe Damsté, Jaap; März, Christian; Brumsack, Hans; Reichart, Gert-Jan

    2010-05-01

    The Azolla interval, as encountered in Eocene sediments from the Arctic Ocean, is characterized by organic rich sediments ( 4wt% Corg). In general, high levels of organic matter may be caused by increased productivity, i.e. extensive growth of Azolla, and/or enhanced preservation of organic matter, or a combination of both. Anoxic (bottom) water conditions, expanded oxygen minimum zones, or increased sedimentation rates all potentially increase organic matter preservation. According to plate tectonic, bathymetric, and paleogeographic reconstructions, the Arctic Ocean was a virtually isolated shallow basin, with one possible deeper connection to the Nordic Seas represented by a still shallow Fram Strait (Jakobsson et al., 2007), hampering ventilation of the Arctic Basin. During the Azolla interval surface waters freshened, while at the same time bottom waters appear to have remained saline, indicating that the Arctic was highly stratified. The restricted ventilation and stratification in concert with ongoing export of organic matter most likely resulted in the development of anoxic conditions in the lower part of the water column. Whereas the excess precipitation over evaporation maintained the freshwater lid, sustained input of Nordic Sea water is needed to keep the deeper waters saline. To which degree the Arctic Ocean exchanged with the Nordic Seas is, however, still largely unknown. Here we present a high-resolution trace metal record (ICP-MS and ICP-OES) for the expanded Early/Middle Eocene section capturing the Azolla interval from Integrated Ocean Drilling Program (IODP) Expedition 302 (ACEX) drilled on the Lomonosov Ridge, central Arctic Ocean. Euxinic conditions throughout the interval resulted in the efficient removal of redox sensitive trace metals from the water column. Using the sedimentary trace metal record we also constrained circulation in the Arctic Ocean by assessing the relative importance of trace metal input sources (i.e. fluvial, eolian, and

  7. Palaeoecological and palaeoclimatological implications of the Eocene Northern Hemisphere Azolla phenomenon

    OpenAIRE

    Barke, J

    2010-01-01

    The high abundances and cyclic distribution of remains of the freshwater fern Azolla in early middle Eocene sediments from the Arctic Ocean have previously been related to episodic surface water freshening, which was speculated to be orbitally modulated. Our integrated palynological and cyclostratigraphical analysis of the recovered ‘Azolla interval’ in Integrated Ocean Drilling Program (IODP) Core 302-M0004A-11X resulted in the recognition of two clear periodicities: a dominant ~1.2 m cyclic...

  8. Pengaruh Biomassa Azolla Terhadap Status Logam Berat Timbal (Pb) Pada Tanah

    OpenAIRE

    Abror, Muhammad

    2013-01-01

    Pencemaran tanah akibat logam berat semakin banyak terjadi akibat perkembangan industri dan kurangnya pengawasan terhadap polutan. Tanah tidak terlepas pada ancaman ini. Penelitian pengaruh biomassa azolla terhadap status logam berat timbale (Pb) pada tanah bertujuan untuk mengukur kemampuan bimassa azolla tercemar Pb dalam menambahkan maupun mengurangi ketersediaan Pb pada tanah yang dicemari Pb maupun yang tidak dicemari. Penelitian ini menggunakan rancangan acak kelompok faktorial dengan p...

  9. Classification and phylogeny of the cyanobiont Anabaena azollae Strasburger: an answered question?

    Science.gov (United States)

    Pereira, Ana L; Vasconcelos, Vitor

    2014-06-01

    The symbiosis Azolla-Anabaena azollae, with a worldwide distribution in pantropical and temperate regions, is one of the most studied, because of its potential application as a biofertilizer, especially in rice fields, but also as an animal food and in phytoremediation. The cyanobiont is a filamentous, heterocystic cyanobacterium that inhabits the foliar cavities of the pteridophyte and the indusium on the megasporocarp (female reproductive structure). The classification and phylogeny of the cyanobiont is very controversial: from its morphology, it has been named Nostoc azollae, Anabaena azollae, Anabaena variabilis status azollae and recently Trichormus azollae, but, from its 16S rRNA gene sequence, it has been assigned to Nostoc and/or Anabaena, and from its phycocyanin gene sequence, it has been assigned as non-Nostoc and non-Anabaena. The literature also points to a possible co-evolution between the cyanobiont and the Azolla host, since dendrograms and phylogenetic trees of fatty acids, short tandemly repeated repetitive (STRR) analysis and restriction fragment length polymorphism (RFLP) analysis of nif genes and the 16S rRNA gene give a two-cluster association that matches the two-section ranking of the host (Azolla). Another controversy surrounds the possible existence of more than one genus or more than one species strain. The use of freshly isolated or cultured cyanobionts is an additional problem, since their morphology and protein profiles are different. This review gives an overview of how morphological, chemical and genetic analyses influence the classification and phylogeny of the cyanobiont and future research. PMID:24737795

  10. Integrating fish and azolla into rice-duck farming in Asia

    OpenAIRE

    Cagauan, A.G.; Branckaert, R.D.; van Hove, C.

    2000-01-01

    Several countries in Asia practice integrated rice-duck farming. On-farm resources such as duck manure and feed waste are not adequately used and recycled in the system. This indicates the potential for research to increase the productivity of the rice-duck system. The integration of fish and the nitrogen-fixing aquatic fern azolla show promise for increasing the production potential of the system. Fish, azolla and ducks integrated with rice farming can result in nutrient enhancement, pest co...

  11. The omnipresent water fern Azolla caroliniana does not contain lignin

    OpenAIRE

    Nierop, K. G. J.; Speelman, E. N.; Leeuw, J.W. de; G. J. Reichart

    2011-01-01

    Several studies have reported the presence of large amounts of lignin in ubiquitously occurring species of the freshwater fern Azolla. Molecular analysis using flash pyrolysis and thermally assisted hydrolysis and methylation shows, however, that neither the leaves nor the roots of Azolla contain lignin. Instead, both contain polyphenolic constituents that have a close resemblance to condensed tannins, albeit with a slightly different composition than the common tannins of higher plants.

  12. Removal of contaminants in a paper mill effluent by Azolla caroliniana

    Directory of Open Access Journals (Sweden)

    D. Sivakumar

    2015-09-01

    Full Text Available This study was focused on removal of various parameters in paper mill effluent using a method called bioremediation by Azolla caroliniana.  The experimental investigations have been carried out using Azolla caroliniana for conducting the sorption study with various dilution ratios (2, 4, 6, 8, and 10, pH (3, 4, 5, 6, 7, 8 and 9 and biomass (200, 400, 600, 800 and 1000 g. The maximum removal percentage of TDS, BOD and COD in a paper mill effluent was obtained at the optimum dilution ratio of 6, pH of 8 and biomass of 800 g. The results of this study indicated that the maximum removal percentage of TDS, BOD and COD in a paper mill effluent was 82.3 %, 88.6 % and 79.1 % respectively.  Also, the study focused on uptake of TDS, BOD and COD in paper mill effluent by Azolla caroliniana through bioaccumulation factor and translocation factor. The results of bioaccumulation factor revealed that TDS, BOD and COD in paper mill effluent were adsorbed by Azolla caroliniana.  The results of translocation factor revealed that the roots of Azolla caroliniana translocate the TDS, BOD and COD in a paper mill effluent to the shoots of Azolla caroliniana. From the results, this study concluded that bioremediation by Azolla caroliniana could be effectively used for removing TDS, BOD and COD in a paper mill effluent. This study also suggested that Azolla caroliniana may be used for removing various contaminants, not only from paper mill effluent, but also from any other industrial effluents.

  13. Classification and phylogeny of the cyanobiont Anabaena azollae Strasburger: an answered question?

    Science.gov (United States)

    Pereira, Ana L; Vasconcelos, Vitor

    2014-06-01

    The symbiosis Azolla-Anabaena azollae, with a worldwide distribution in pantropical and temperate regions, is one of the most studied, because of its potential application as a biofertilizer, especially in rice fields, but also as an animal food and in phytoremediation. The cyanobiont is a filamentous, heterocystic cyanobacterium that inhabits the foliar cavities of the pteridophyte and the indusium on the megasporocarp (female reproductive structure). The classification and phylogeny of the cyanobiont is very controversial: from its morphology, it has been named Nostoc azollae, Anabaena azollae, Anabaena variabilis status azollae and recently Trichormus azollae, but, from its 16S rRNA gene sequence, it has been assigned to Nostoc and/or Anabaena, and from its phycocyanin gene sequence, it has been assigned as non-Nostoc and non-Anabaena. The literature also points to a possible co-evolution between the cyanobiont and the Azolla host, since dendrograms and phylogenetic trees of fatty acids, short tandemly repeated repetitive (STRR) analysis and restriction fragment length polymorphism (RFLP) analysis of nif genes and the 16S rRNA gene give a two-cluster association that matches the two-section ranking of the host (Azolla). Another controversy surrounds the possible existence of more than one genus or more than one species strain. The use of freshly isolated or cultured cyanobionts is an additional problem, since their morphology and protein profiles are different. This review gives an overview of how morphological, chemical and genetic analyses influence the classification and phylogeny of the cyanobiont and future research.

  14. Salinity induced physiological and biochemical changes in the freshly separated cyanobionts of Azolla microphylla and Azolla caroliniana.

    Science.gov (United States)

    Yadav, Ravindra Kumar; Tripathi, Keshawanand; Ramteke, Pramod Wasudeo; Varghese, Eldho; Abraham, Gerard

    2016-09-01

    Freshly separated cyanobionts of Azolla microphylla and Azolla caroliniana plants exposed to salinity showed decline in the cellular constituents such as chlorophyll (23.1 and 38.9%) and protein (12.9 and 19.3%). However, an increase in the carotenoid and sugar content was observed. Exposure to salinity stress reduced the heterocyst frequency (35.4 and 57.2%) and nitrogenase activity (37.7 and 46.3%) of the cyanobionts. Increase in the activity of antioxidant enzymes such as super oxide dismutase (50.6 and 11.5%), ascorbate peroxidase (63.7 and 57.9%), catalase (94.2 and 22.5%) as well as non-enzymatic antioxidant proline (18.8 and 13.3%) was also observed in response to salinity. The cyanobionts exhibited significant increase in the intracellular Na(+) level and reduced intracellular K(+)/Na(+) and Ca(2+)/Na(+) ratio in response to salinity. The results demonstrate the adverse impact of salinity on the freshly separated cyanobionts as similar to free living cyanobacteria. These results may be helpful in the critical evaluation of salinity tolerance mechanism of the cyanobiont and its interaction with the host. PMID:27135817

  15. Azolla pinnata growth performance in different water sources.

    Science.gov (United States)

    Nordiah, B; Harah, Z Muta; Sidik, B Japar; Hazma, W N Wan

    2012-07-01

    Azolla pinnata R.Br. growth performance experiments in different water sources were conducted from May until July 2011 at Aquaculture Research Station, Puchong, Malaysia. Four types of water sources (waste water, drain water, paddy field water and distilled water) each with different nutrient contents were used to grow and evaluate the growth performance of A. pinnata. Four water sources with different nutrient contents; waste, drain, paddy and distilled water as control were used to evaluate the growth performance of A. pinnata. Generally, irrespective of the types of water sources there were increased in plant biomass from the initial biomass (e.g., after the first week; lowest 25.2% in distilled water to highest 133.3% in drain water) and the corresponding daily growth rate (3.61% in distilled water to 19.04% in drain water). The increased in biomass although fluctuated with time was consistently higher in drain water compared to increased in biomass for other water sources. Of the four water sources, drain water with relatively higher nitrate concentration (0.035 +/- 0.003 mg L(-l)) and nitrite (0.044 +/- 0.005 mg L(-1)) and with the available phosphate (0.032 +/- 0.006 mg L(-1)) initially provided the most favourable conditions for Azolla growth and propagation. Based on BVSTEP analysis (PRIMER v5), the results indicated that a combination of more than one nutrient or multiple nutrient contents explained the observed increased in biomass of A. pinnata grown in the different water sources. PMID:24218931

  16. INFLUENCE OF AZOLLA-ANABAENA SYMBIOSIS ON RICE (Oryza sativa L CROP AS A NUTRITIONAL ALTERNATIVE

    Directory of Open Access Journals (Sweden)

    R. Castro

    2003-01-01

    Full Text Available Con el objetivo de estudiar la influencia de la asociación o incorporación de Azolla en el cultivo del arroz en condiciones controladas, se llevó a cabo un experimento durante el período comprendido entre mayo y octubre de 1997, usando un diseño completamente aleatorizado. Se evaluaron 16 tratamientos, productos de la combinación de cuatro niveles de nitrógeno (0, 40, 80, 120 kg N.ha-1 y cuatro sistemas de cultivo (arroz en monocultivo, arroz+Azolla incorporada, arroz+Azolla incorporada+Azolla asociada, arroz+Azolla asociada y cada tratamiento fue replicado cuatro veces. Los resultados mostraron que el arroz fue influido de forma positiva por el uso de Azolla en todas las variantes usadas, destacándose la combinación de incorporación del helecho y la asociación de este con el cultivo del arroz, lo que permitió incrementar la altura de las plantas, el número de hijos fértiles, el área foliar y su masa seca, así como provocó un aumento notable en la extracción de nutrientes por este cereal. El uso de Azolla incrementó los contenidos de materia orgánica y potasio del suelo. Se observó también un efecto positivo al incrementar la dosis de fertilización nitrogenada.

  17. The potential of the aquatic water fern Azolla within a biobased economy

    Science.gov (United States)

    Nierop, Klaas G. J.; Jongerius, Anna L.; Bijl, Peter K.; Bruijnincx, Pieter C. A.; Klein Gebbink, Robertus J. M.; Reichart, Gert-Jan

    2014-05-01

    Azolla is a free-floating freshwater fern capable of fixing atmospheric carbon dioxide and nitrogen, the latter of which through its symbiosis with the cyanobacteria Anabaena azollae. It is currently ranked among the fastest growing plants on Earth and occurs in both tropical and temperate freshwater ecosystems. Therefore, it is non-directly competitive with food crops. In addition, Azolla does not require inorganic fertilizers, which makes it a potential and unique source of biomass for the sustainable production of fuels and chemicals that are currently derived from fossil (fuel) sources. The biochemical composition of Azolla allows the production of biofuel or biobased chemicals that are of interest to the chemical industry. Of Azolla, two extractable groups of compounds are of particular interest, i.e. the polyphenols (condensed tannins and ester-bound caffeic acid) and the lipids. The antioxidant property of polyphenols and their application to the treatment of cancer, diabetes and cardiovascular diseases has further contributed to the growth of the polyphenol market. In addition, they can be chemically transformed into aromatic platform and specialty chemicals. The composition of the lipid fraction of Azolla is characterized by highly specific compounds consisting of C26-C36 carbon chains all bearing a ω20-hydroxy group. Such compounds produce an oil fraction upon hydrous pyrolysis, or, alternatively, are well suited to be converted to e.g. various specialty chemicals that are hardly available from both natural sources. Indeed, upon chemical conversion these lipids may yield components for fuels, plastics, cosmetics, and lubricants. Another group of interesting compounds within the lipid group are the polyunsaturated fatty acids (PUFAs). The demand for PUFAs has witnessed a significant increase over the last three years, particularly due to their benefits as cholesterol lowering agents. Here we will present some of the thermal and chemical conversions of the

  18. Breakdown of middle lamella pectin by (●) OH during rapid abscission in Azolla.

    Science.gov (United States)

    Yamada, Yoshiya; Koibuchi, Mizuki; Miyamoto, Kensuke; Ueda, Junichi; Uheda, Eiji

    2015-08-01

    Azolla, a small water fern, abscises its roots and branches within 30 min upon treatment with various stresses. This study was conducted to test whether, in the rapid abscission that occurs in Azolla, breakdown of wall components of abscission zone cells by (●) OH is involved. Experimentally generated (●) OH caused the rapid separation of abscission zone cells from detached roots and the rapid shedding of roots from whole plants. Electron microscopic observations revealed that (●) OH rapidly and selectively dissolved a well-developed middle lamella between abscission zone cells and resultantly caused rapid cell separation and shedding. Treatment of abscission zones of Impatiens leaf petiole with (●) OH also accelerated the separation of abscission zone cells. However, compared with that of Azolla roots, accelerative effects in Impatiens were weak. A large amount of (●) OH was cytochemically detected in abscission zone cells both of Azolla roots and of Impatiens leaf petioles. These results suggest that (●) OH is involved in the cell separation process not only in the rapid abscission in Azolla but also in the abscission of Impatiens. However, for rapid abscission to occur, a well-developed middle lamella, a unique structure, which is sensitive to the attack of (●) OH, might be needed. PMID:25581142

  19. EFECTO DEL USO DE Azolla EN LOS RENDIMIENTOS DE CULTIVOS EN CONDICIONES DE ORGANOPÓNICOS

    Directory of Open Access Journals (Sweden)

    R. Castro

    2006-01-01

    Full Text Available En el período comprendido entre 1996 y 2001, se realizó el trabajo integrado por dos experimentos, en el organopónico del INRE, Los Palacios, Pinar del Río, con el objetivo de evaluar la influencia de la dosis de Azolla y el efecto residual sobre el rendimiento y la evolución del sustrato de los canteros de los organopónicos con aplicaciones periódicas de este helecho; para ello, se emplearon los cultivos de lechuga y cebollino. En el cultivo de la lechuga se evaluó el efecto de seis dosis de Azolla durante seis siembras consecutivas, comparándolo con un testigo; los resultados indican que la utilización de Azolla incrementa los rendimientos y sus componentes, obteniéndose los mayores valores en materias fresca y seca con aplicaciones mayores de 1.5 kg.m-2, cuando el sustrato es rico en materia orgánica, y con aplicaciones superiores a 2 kg.m-2 de Azolla, cuando el sustrato está muy degradado. Además, el uso de Azolla disminuye la caída del contenido de materia orgánica y, por lo tanto, de los rendimientos de los cultivos de lechuga y cebollino.

  20. Separation of abscission zone cells in detached Azolla roots depends on apoplastic pH.

    Science.gov (United States)

    Fukuda, Kazuma; Yamada, Yoshiya; Miyamoto, Kensuke; Ueda, Junichi; Uheda, Eiji

    2013-01-01

    In studies on the mechanism of cell separation during abscission, little attention has been paid to the apoplastic environment. We found that the apoplastic pH surrounding abscission zone cells in detached roots of the water fern Azolla plays a major role in cell separation. Abscission zone cells of detached Azolla roots were separated rapidly in a buffer at neutral pH and slowly in a buffer at pH below 4.0. However, cell separation rarely occurred at pH 5.0-5.5. Light and electron microscopy revealed that cell separation was caused by a degradation of the middle lamella between abscission zone cells at both pH values, neutral and below 4.0. Low temperature and papain treatment inhibited cell separation. Enzyme(s) in the cell wall of the abscission zone cells might be involved in the degradation of the pectin of the middle lamella and the resultant, pH-dependent cell separation. By contrast, in Phaseolus leaf petioles, unlike Azolla roots, cell separation was slow and increased only at acidic pH. The rapid cell separation, as observed in Azolla roots at neutral pH, did not occur. Indirect immunofluorescence microscopy, using anti-pectin monoclonal antibodies, revealed that the cell wall pectins of the abscission zone cells of Azolla roots and Phaseolus leaf petioles looked similar and changed similarly during cell separation. Thus, the pH-related differences in cell separation mechanisms of Azolla and Phaseolus might not be due to differences in cell wall pectin, but to differences in cell wall-located enzymatic activities responsible for the degradation of pectic substances. A possible enzyme system is discussed. PMID:22940290

  1. Effect of cerium application on aniline wastewater degradation by Azolla filiculoides Lamk and Salvinia natans%铈对满江红和槐叶萍降解苯胺类废水的效果分析

    Institute of Scientific and Technical Information of China (English)

    邵波

    2013-01-01

    选取满江红和槐叶萍两种浮水植物为研究对象,通过在同等条件下的室内静态模拟试验,采用高效液相色谱法测定,分析比较了它们在稀土铈的施用下对废水中苯胺类化合物的降解效果.研究表明:满江红和槐叶萍对受试水体中的对硝基苯胺、2,4-硝基苯胺和3-硝基苯胺均有降解效果.微量的稀土元素铈能促进植物对三类物质的降解,其中浓度为5 mg·L-1的稀土溶液促进槐叶萍效果最佳,降解率分别达到89.60%,89.86%和89.69%,但高浓度时出现抑制.满江红总体去除效果要优于槐叶萍.

  2. The Eocene Arctic Azolla phenomenon: species composition, temporal range and geographic extent.

    Science.gov (United States)

    Collinson, Margaret; Barke, Judith; van der Burgh, Johan; van Konijnenburg-van Cittert, Johanna; Pearce, Martin; Bujak, Jonathan; Brinkhuis, Henk

    2010-05-01

    Azolla is a free-floating freshwater fern that is renowned for its rapid vegetative spread and invasive biology, being one of the world's fastest growing aquatic macrophytes. Two species of this plant have been shown to have bloomed and reproduced in enormous numbers in the latest Early to earliest Middle Eocene of the Arctic Ocean and North Sea based on samples from IODP cores from the Lomonosov Ridge (Arctic) and from outcrops in Denmark (Collinson et al 2009 a,b Review of Palaeobotany and Palynology 155,1-14; and doi:10.1016/j.revpalbo.2009.12.001). To determine the geographic and temporal extent of this Azolla phenomenon, and the spatial distribution of the different species, we have examined samples from 15 additional sites using material from ODP cores and commercial exploration wells. The sites range from the Sub-Arctic (Northern Alaska and Canadian Beaufort Mackenzie Basin) to the Nordic Seas (Norwegian-Greenland Sea and North Sea Basin). Our data show that the Azolla phenomenon involved at least three species. These are distinguished by characters of the megaspore apparatus (e.g. megaspore wall, floats, filosum) and the microspore massulae (e.g. glochidia fluke tips). The Lomonosov Ridge (Arctic) and Danish occurrences are monotypic but in other sites more than one species co-existed. The attachment to one another and the co-occurrence of megaspore apparatus and microspore massulae, combined with evidence that these spores were shed at the fully mature stage of their life cycle, shows that the Azolla remains were not transported over long distances, a fact which could not be assumed from isolated massula fragments alone. Our evidence, therefore, shows that Azolla plants grew on the ocean surfaces for approximately 1.2 million years (from 49.3 to 48.1 Ma) and that the Azolla phenomenon covered the area from Denmark northwards across the North Sea Basin and the whole of the Arctic and Nordic seas. Apparently, early Middle Eocene Northern Hemisphere middle

  3. Azolla pinnata as a possible replacement of palm kernel cake in the diet of oreochromis niloticus.

    OpenAIRE

    Abioye, O.M.; Adeyemo, A.A.; Ayinla, O.A.; Bekibele, D.O.

    1993-01-01

    Azolla pinnata as a possible replacement of the palm kernel cake in the diet of Oreochromis niloticus. Compounded diets from a control with palm kernel cake replaced at varying levels of 0, 20, 40, 60, 80, and 100% with the aquatic fern (oven dried Azolla pinnata) were fed to Oreochromis niloticus for twelve weeks to determine the acceptability and utilization of the fern in the diet, verify the nutritional influence of the fern on the performance of the fish and possible optimum palm kernel ...

  4. Removal of contaminants in a paper mill effluent by Azolla caroliniana

    OpenAIRE

    Sivakumar, D.; J. Nouri

    2015-01-01

    This study was focused on removal of various parameters in paper mill effluent using a method called bioremediation by Azolla caroliniana.  The experimental investigations have been carried out using Azolla caroliniana for conducting the sorption study with various dilution ratios (2, 4, 6, 8, and 10), pH (3, 4, 5, 6, 7, 8 and 9) and biomass (200, 400, 600, 800 and 1000 g). The maximum removal percentage of TDS, BOD and COD in a paper mill effluent was obtained at the optimum dilution ratio o...

  5. USO DEL GÉNERO AZOLLA COMO BIOFERTILIZANTE EN EL CULTIVO DEL ARROZ (Oryza sativa L.)

    OpenAIRE

    Castro, R; R. Novo; R. I. Castro

    2002-01-01

    Con el objetivo de evaluar los beneficios que proporciona la asociación arroz-Azolla al cultivo del arroz en Cuba, se desarrollaron estos trabajos durante el período comprendido entre marzo y julio de 1998, en la Estación Experimental del Arroz "Los Palacios", ubicada en el municipio Los Palacios en la provincia de Pinar del Río, sobre un suelo Hidromórfico Gley Nodular Ferruginoso Petroférrico. En el trabajo se evalúo la influencia de Azolla sobre el cultivo del arroz. Los resultados mostra...

  6. Azolla-anabaena-bacteria system as a natural microcosm

    Science.gov (United States)

    Carrapico, Francisco J.

    2002-02-01

    Azolla is an aquatic fern that contains a permanent endosymbiotic prokaryotic community (cyanobacteria and bacteria) inside of the cavity in the leaf dorsal lobe of the pteridophyte. This is a unique situation and can be seen as a microcosm inside of an organism and also can be considered a good example of a living model for biological and environmental studies. These symbionts are specific of this symbiosis and lives immobilized in a mucilaginous fibrillar network, which fills part of the cavity. The symbionts works as immobilized organisms in a natural system that can be used as a model for biotechnological research and in biologically based life support systems. The nature and the complexity of this system is simultaneously a reference and a challenge for the research in the communication between the two levels of nature organization (microcosm and mesocosm), and can also be used as a reference for the design of new environmental engineered symbiotic systems that include man as a prelude to life in space.

  7. Determination of thermal stability of specific biomarker lipids of the freshwater fern Azolla through hydrous pyrolysis

    Science.gov (United States)

    Sap, Merel; Speelman, Eveline N.; Lewan, Michael D.; Sinninghe Damsté, Jaap S.; Reichart, Gert-Jan

    2010-05-01

    Enormous blooms of the free-floating freshwater fern Azolla occurred within the Arctic Basin during an extended period of ~1.2 Ma during the middle Eocene (Brinkhuis et al. 2006; Speelman et al., GB, 2009). The sustained growth of Azolla, currently ranking among the fastest growing plants on Earth, in a major anoxic basin may have substantially contributed to decreasing atmospheric CO2 levels by burial of Azolla-derived organic matter. Speelman et al. (OG, 2009) reported biomarkers for Azolla (1,w20 C32 - C36 diols, structurally related C29 ω20,ω21 diols, C29 1,20,21 triols, C29 dihydroxy fatty acids as well as a series of wax esters containing these mono- and dihydroxy lipids), which can be used to reconstruct palaeo-environmental conditions. Here we assess the thermal stability of these compounds, to extend their biomarker potential. We specifically focused on the thermal stability of the Azolla biomarkers using hydrous pyrolysis in order to determine which burial conditions allow reconstruction of past occurrences of Azolla. In addition, hydrous pyrolysis was also performed on samples from the Eocene Arctic Ocean (ACEX core), to test if and how the biomarkers change under higher temperatures and pressures in situ. During hydrous pyrolysis, the biomass was heated under high pressure at temperatures ranging between 220 and 365°C for 72 hours. Four experiments were also run using different durations to explore the kinetics of biomarker degradation at specific temperatures. First results indicate that the Azolla specific diols are still present at 220°C, while the corresponding wax esters are already absent. At 300°C all Azolla specific biomarkers are destroyed. More specific determination of the different biomarkers' stability and kinetics would potentially allow the reconstruction of the temperature and pressure history of Azolla deposits. Literature: • Brinkhuis, H., Schouten, S., Collinson, M. E., Sluijs, A., Sinninghe Damste, J. S., Dickens, G. R., Huber

  8. Effects of substituting fish meal with Azolla pinnata in practical diets for fingerling and adult Nile tilapia, Oreochromis niloticus (L.)

    OpenAIRE

    EL-SAYED, A.-F. M.

    1992-01-01

    Dry and fresh Azolla pinnata were evaluated as feed ingredients for fingerling and adult Nile tilapia, Oreochromis niloticus (L.). Dry Azolla was incorporated into practical diets containing approximately 30% crude protein and 360�400kcal/lOOg of GE lo replace 25,50,75 and 100% of the fish meal (FM) protein in the control diet. Fresh Azolla was also tested as a total diet for these fish. Formulated diets were fed to duplicate groups of fingerling (2�54g � 0093) and adult (4033g � 103) fish at...

  9. Effect of azolla-incorporated diets on the growth and survival of Labeo fimbriatus during fry-to-fingerling rearing

    OpenAIRE

    B. Gangadhar; Sridhar, N.; Saurabh, S.; Raghavendra, C.H.; K.P. Hemaprasanth; M.R. Raghunath; Jayasankar, P.

    2015-01-01

    An experiment of 75 days duration was conducted in fertilized outdoor circular cement tanks (1,000 L) with soil base for evaluating the growth and survival of Labeo fimbriatus fry fed with pelleted feed containing varied levels of dried azolla (Azolla pinnata). The Control feed contained 45% groundnut oilcake plus 45% rice bran and 10% finger millet flour added as binder for pelleting. Dried azolla powder was incorporated into the feed at 10, 20, 30 and 40% levels, replacing the groundnut cak...

  10. Validación de un método de análisis para la determinación de metales pesados en partículas pM10 colectadas en aire ambiente

    Directory of Open Access Journals (Sweden)

    Jorge Herrera-Murillo

    2010-10-01

    Full Text Available Se validó un método de análisis para la determinación del contenido de metales presentes en partículas PM10 colectadas del aire ambiente de la ciudad de San José.Las muestras se tomaron utilizando dos muestreadores de aire de alto volumen con controlador de flujo, sobre filtros de cuarzo (20,3 cm x 25,4 cm, con un flujo promedio de 1,13 m3min-1 durante 24 h en dos sitios de la ciudad capital: Catedral Metropolitana y Junta de Educación de San José.  Para la determinación se utilizó espectrofotometría de absorción atómica con horno de grafito.Al analizar las muestras colectadas se determinó que las especies metálicas más abundantes son: Al, Fe y Mn; sin embargo solamente el Pb y Cu presentan factores de enriquecimiento importantes debido al posible aporte de actividades antropogénicas en la ciudad.

  11. Limited Multiplication of Symbiotic Cyanobacteria of Azolla spp. on Artificial Media

    OpenAIRE

    Tang, L F; Watanabe, I.(Graduate School of Science, Kobe University, Kobe, Japan); Liu, C C

    1990-01-01

    We examined various media and conditions to isolate symbiotic cyanobacteria from the leaf cavities of Azolla spp. Cyanobacteria survived and multiplied to a limited extent on a medium with fructose, Casamino Acids, yeast extract, and NaNO3 under 1% O2. These cyanobacteria were antigenically identical to the endosymbionts.

  12. Palaeoecological and palaeoclimatological implications of the Eocene Northern Hemisphere Azolla phenomenon

    NARCIS (Netherlands)

    Barke, J.

    2010-01-01

    The high abundances and cyclic distribution of remains of the freshwater fern Azolla in early middle Eocene sediments from the Arctic Ocean have previously been related to episodic surface water freshening, which was speculated to be orbitally modulated. Our integrated palynological and cyclostratig

  13. Phytoremediation of Hg and Cd from industrial effluents using an aquatic free floating macrophyte Azolla pinnata.

    Science.gov (United States)

    Rai, Prabhat Kumar

    2008-01-01

    The level of heavy metal pollution in Singrauli, an industrial region in India, was assessed and the phytoremediation capacity of a small water fern, Azolla pinnata R.BR (Azollaceae), was observed to purify waters polluted by two heavy metals, i.e., mercury (Hg) and cadmium (Cd) under a microcosm condition. Azolla pinnata is endemic to India and is an abundant and easy-growing free-floating water fern usually found in the rice fields, polluted ponds, and reservoirs of India. The fern was grown in 24 40-L aquariums containing Hg2+ and Cd2+ ions each in concentrations of 0.5, 1.0, and 3.0 mgL(-1) during the course of this study. The study revealed an inhibition of Azolla pinnata growth by 27.0-33.9% with the highest in the presence of Hg (II) ions at 0.5 mgL(-1) in comparison to the control After 13 days of the experiment, metal contents in the solution were decreased up to 70-94%. In the tissues of Azolla pinnata, the concentration of selected heavy metals during investigation was recorded between 310 and 740 mgKg(-1) dry mass, with the highest levelfoundfor Cd (II) treatment at 3.0 mgL(-1) containing a metal solution. PMID:19260224

  14. Orbitally forced Azolla blooms And Middle Eocene Arctic hydrology: Clues from palynology

    NARCIS (Netherlands)

    Barke, J.; Abels, H.A.; Sangiorgi, F.; Greenwood, D.R.; Sweet, A.R.; Donders, T.; Reichart, G.-J.; Lotter, A.F.; Brinkhuis, H.

    2011-01-01

    The high abundances and cyclic distribution of remains of the freshwater fern Azolla in early-Middle Eocene sediments from the Arctic Ocean have previously been related to episodic surface-water freshening, which was speculated to be orbitally modulated. Our integrated palynological and cyclostratig

  15. Endosulfan induced changes in growth rate, pigment composition and photosynthetic activity of mosquito fern Azolla microphylla

    Directory of Open Access Journals (Sweden)

    Raja W.

    2012-11-01

    Full Text Available This paper is the first in a series reporting a study on the effects of different concentrations of insecticide, Endosulfan (0-600ppm was premeditated on 5th day after insecticide exposure with respect to growth rate, pigment composition and photosynthetic activity of Azolla microphylla under laboratory conditions which become non-target organism in the rice fields. Endosulfan inhibited the relative growth rate, pigment content and photosynthetic O2 evolution. Phycocyanin was main target followed by carotenoid and total chlorophyll. Significant increase in pigment, flavonoid and Anthocyanin was noticed after six days of treatment. In contrast to the photosynthetic activity, the rate of respiration in Azolla microphylla was increased significantly. Our results show that Endosulfan at normally recommended field rates and intervals are seldom deleterious to the beneficial and Eco friendly Azolla microphylla and their activities and thus in turn suppress plant growth and development. Phytotoxity of Azolla microphylla can be minimized by restrictions on application, timing, method and rate of application.

  16. Azolla-Anabaena as a Biofertilizer for Rice Paddy Fields in the Po Valley, a Temperate Rice Area in Northern Italy

    OpenAIRE

    Stefano Bocchi; Antonino Malgioglio

    2010-01-01

    Azolla is a floating pteridophyte, which contains as endosymbiont the nitrogen-fixing cyanobacterium Anabaena azollae (Nostocaceae family). Widely cultivated in the Asian regions, Azolla is either incorporated into the soil before rice transplanting or grown as a dual crop along with rice. To examine the feasibility of its use in flooded rice fields sited in the Temperate European Areas, we carried out a series of experiments in PVC tanks during 2000–2002 in Po Valley (northern Italy) conditi...

  17. Reconstruction of the Arctic Ocean environment during the Eocene Azolla interval using geochemical proxies and climate modeling. Geologica Ultraiectina (331)

    OpenAIRE

    Speelman, E. N.

    2010-01-01

    With the realization that the Arctic Ocean was covered with enormous quantities of the aquatic floating fern Azolla 49 Myrs ago, new questions regarding the Eocene conditions facilitating these blooms arose. This dissertation describes the reconstruction of paleo-environmental conditions facilitating the large-scale occurrence of the freshwater fern Azolla in the Early/Middle Eocene Arctic and how this bloom might have affected global climate. Comparison of organic geochemical analyses of Eoc...

  18. Exploring the effects of inclusion of dietary fresh Azolla on the performance of White Pekin broiler ducks

    OpenAIRE

    Parag Acharya; Mohanty, G. P.; Pradhan, C. R.; Mishra, S. K.; Beura, N. C.; Moharana, B.

    2015-01-01

    Aim: The objective of this study was to investigate the dietary advantages of inclusion of unconventional nutrient source, i.e., Azolla in the basal diet of White Pekin broilers. Materials and Methods: All ducklings were randomly distributed into three treatment groups with three replicates each and each replicate having 12 ducklings and were reared in deep litter system. Groups were subjected to three dietary treatments such as G1: Basal diet, G2: Basal diet + 5% fresh Azolla of basal die...

  19. Combined use of Azolla and loach suppressed paddy weeds and increased organic rice yield: second season results

    Directory of Open Access Journals (Sweden)

    Weiguo Cheng

    2015-07-01

    Full Text Available Organic farming uses alternatives to agricultural chemicals such as synthetic fertilizers and pesticides. The primary challenge in organic rice farming is controlling weeds without using herbicides and improving rice yield without chemical fertilizers. In our previous paper entitled as combined use of Azolla and loach suppressed weed Monochoria vaginalis and increased rice yield without agrochemicals, we reported the first year rice growth season results from an in situ container experiment. The experiment was designed with 4 treatments—control (with neither Azolla nor loach, Azolla (Azolla alone, loach (loach alone, and Az+Lo (combined Azolla and loach—with 3 replications each. The first year results showed that combined use of Azolla and loach was successful in weed suppression and increase in rice yield in 2012. In this paper, we report the second year results from the continuous container experiment in 2013. M.vaginalis emergences were very low in second year rice growth season on all treatments. Compared first year, the rice yields decreased in second year on all treatments due to different weather condition and with or without organic soybean oil cake application between two rice growth seasons. The second year results also showed the raising loach had a stronger effect to increase tiller and panicle numbers, and spikelet number per panicle, then improve rice yields to 2.3 times than control. The Azolla residues left from first year have weaker effect on rice growth and yield, but increase soil organic matter accumulation at second year. The two years study indicated that combined use of Azolla and loach can meet two of the greatest challenges in organic rice production: providing effective weed control and improving rice nutrition without agrochemicals.

  20. Effect of partial supplementation of sun-dried Azolla as a protein source on the immunity and antioxidant status of commercial broilers

    OpenAIRE

    Biswal Chichilichi; Mohanty, G. P.; Mishra, S. K.; Pradhan, C. R.; Behura, N. C.; Das, A.; K. Behera

    2015-01-01

    Aim: The present study was conducted to evaluate the effect of partial supplementation of sun-dried Azolla as a protein source on the immunity of commercial broilers in coastal Odisha. Materials and Methods: A 180 day-old broiler chicks were distributed in six dietary treatments viz. C1: Basal diet, C2: Basal diet + enzyme, T1: Basal diet +5% protein from Azolla, T2: Basal diet + 5% protein from Azolla + enzyme, T3: Basal diet +10% protein from Azolla, and T4: Basal diet + 10% protein from Az...

  1. Effect of azolla-incorporated diets on the growth and survival of Labeo fimbriatus during fry-to-fingerling rearing

    Directory of Open Access Journals (Sweden)

    B. Gangadhar

    2015-12-01

    Full Text Available An experiment of 75 days duration was conducted in fertilized outdoor circular cement tanks (1,000 L with soil base for evaluating the growth and survival of Labeo fimbriatus fry fed with pelleted feed containing varied levels of dried azolla (Azolla pinnata. The Control feed contained 45% groundnut oilcake plus 45% rice bran and 10% finger millet flour added as binder for pelleting. Dried azolla powder was incorporated into the feed at 10, 20, 30 and 40% levels, replacing the groundnut cake and rice bran proportionately. L. fimbriatus (mean length 2.42 cm fry were stocked in all the tanks at 30 m−3. The fish were fed 10% of body weight during the first month, followed by 7% during the second month and 5% during the last 15 days. Incorporation of azolla did not affect (p > 0.05 the water quality, growth and survival of fingerlings at harvest. Incorporation of azolla in the diet reduced the cost of feed (Rs. per 100 g biomass; Rs: Indian rupee, INR; 1 INR ≈ 0.015 EUR from 3.35 to 2.53, with a cost saving of 24.48%. The study indicated the possibility of incorporating azolla in diets of L. fimbriatus up to 40% during fry-to-fingerling rearing, resulting in savings on feed cost.

  2. The aquatic fern Azolla as a natural plant-factory for ammonia removal from fish-breeding fresh wastewater.

    Science.gov (United States)

    Carlozzi, Pietro; Padovani, Giulia

    2016-05-01

    This study has investigated the potential of an Azolla-Anabaena symbiosis, a marriage between the cyanobacterium Anabaena azollae and the aquatic fern (Azolla), to remove ammonia from freshwater fish breeding areas. Experiments were carried out under artificial light of 20, 70, and 140 μmol m(-2) s(-1). We investigated three different water temperatures for the growing Azolla, ranging from sub-optimal to optimal temperatures (15, 22, and 28 °C). The capability of Azolla to remove ammonia from wastewater was demonstrated, and the highest ammonia concentration tolerated by the symbiosis between Azolla-anabaena without any toxic effect on the aquatic ferns was ascertained. The shortest time taken to remove ammonia from wastes, 2.5 cm deep and at 28 °C, was 40 min. The ammonia removal rate (A RR) was both light and temperature dependent and the highest rate (6.394 h(-1)) was attained at light intensity of 140 μmol m(-2) s(-1) and at a temperature of 28 °C; the lowest (0.947 h(-1)) was achieved at 20 μmol m(-2) s(-1) and 15 °C. The depth of the fish-wastewater pool also affected the A RR with the relation between A RR and the depth being a hyperbolic function. PMID:26805923

  3. DNA Probes Show Genetic Variation in Cyanobacterial Symbionts of the Azolla Fern and a Closer Relationship to Free-Living Nostoc Strains than to Free-Living Anabaena Strains

    OpenAIRE

    Plazinski, Jacek; Zheng, Qi; Taylor, Rona; Croft, Lynn; Rolfe, Barry G.; Gunning, Brian E. S.

    1990-01-01

    Twenty-two isolates of Anabaena azollae derived from seven Azolla species from various geographic and ecological sources were characterized by DNA-DNA hybridization. Cloned DNA fragments derived from the genomic sequences of three different A. azollae isolates were used to detect restriction fragment length polymorphism among all symbiotic anabaenas. DNA clones were radiolabeled and hybridized against southern blot transfers of genomic DNAs of different isolates of A. azollae digested with re...

  4. Exploring the effects of inclusion of dietary fresh Azolla on the performance of White Pekin broiler ducks

    Directory of Open Access Journals (Sweden)

    Parag Acharya

    2015-11-01

    Full Text Available Aim: The objective of this study was to investigate the dietary advantages of inclusion of unconventional nutrient source, i.e., Azolla in the basal diet of White Pekin broilers. Materials and Methods: All ducklings were randomly distributed into three treatment groups with three replicates each and each replicate having 12 ducklings and were reared in deep litter system. Groups were subjected to three dietary treatments such as G1: Basal diet, G2: Basal diet + 5% fresh Azolla of basal diet, G3: Basal diet + 10% fresh Azolla of basal diet. During experimental period, parameters, such as bodyweight, bodyweight gain, feed consumption, feed conversion ratio (FCR, and economic of production, were recorded. Results: The result showed that body weights in every week, except the 5th week, did not differ significantly between groups. However, no significant difference was observed between the groups in any week in terms of absolute body weight gain and feed consumption. As regard to FCR, the 10% Azolla group (G3 showed the best feed efficiency followed by the 5% Azolla group (G2 and the control group (G1. On the basis of profit/bird or profit/kg live weight, each of the Azolla-fed group showed a higher economic efficiency than the control. Conclusion: From the experiment, it was concluded that Azolla at 5 or 10% level can be included in the diet to economize the White Pekin broiler farming, and it can be great impetus to poultry farming to include unconventional nutrient source as a dietary supplement.

  5. Azolla as a component of the space diet during habitation on Mars

    Science.gov (United States)

    Katayama, Naomi; Yamashita, Masamichi; Kishida, Yoshiro; Liu, Chung-Chu; Watanabe, Iwao; Wada, Hidenori; Space Agriculture Task Force

    We evaluate a candidate diet and specify its space agricultural requirements for habitation on Mars. Rice, soybean, sweet potato and a green-yellow vegetable have been selected as the basic vegetarian menu. The addition of silkworm pupa, loach, and Azolla to that basic menu was found to meet human nutritional requirements. Co-culture of rice, Azolla, and loach is proposed for developing bio-regenerative life support capability with high efficiency of the usage of habitation and agriculture area. Agriculture designed under the severe constraints of limited materials resources in space would make a positive contribution toward solving the food shortages and environmental problems facing humans on Earth, and may provide an effective sustainable solution for our civilization.

  6. Ecotoxicidade de herbicidas para a macrófita aquática (Azolla caroliniana) Ecotoxicity of herbicides for the aquatic macrophyte (Azolla caroliniana)

    OpenAIRE

    A.F. Silva; Cruz, C.; A. N. Neto; R.A. Pitelli

    2012-01-01

    Os objetivos deste estudo foram avaliar Azolla caroliniana como planta-teste em estudos ecotoxicológicos e estimar a CL50;7d dos herbicidas 2,4-D, glyphosate, clomazone e oxyfluorfen. As plantas foram aclimatadas em sala de bioensaio. Para isso, foram selecionadas cinco plantas em 50 mL de meio de cultivo Hoagland. Após esse período, foram adicionados 50 mL de Hoagland mais o herbicida, completando o volume para 100 mL. A concentração letal de 50% (CL50;7d) para A. caroliniana exposta ao herb...

  7. ENDOSULFAN INDUCED CHANGES IN GROWTH RATE, PIGMENT COMPOSITION AND PHOTOSYNTHETIC ACTIVITY OF MOSQUITO FERN AZOLLA MICROPHYLLA

    OpenAIRE

    Raja W.; Rathaur P.; John S. A.; Ramteke P. W.

    2012-01-01

    This paper is the first in a series reporting a study on the effects of different concentrations of insecticide, Endosulfan (0-600ppm) was premeditated on 5th day after insecticide exposure with respect to growth rate, pigment composition and photosynthetic activity of Azolla microphylla under laboratory conditions which become non-target organism in the rice fields. Endosulfan inhibited the relative growth rate, pigment content and photosynthetic O2 evolution. Phycocyanin was main target fo...

  8. A preliminary examination of the phytochemical profile of Azolla microphylla with respect to Seasons

    Institute of Scientific and Technical Information of China (English)

    G. Abraham; Vidhu Aeri

    2012-01-01

    Objective: To evaluate the role of seasons on the phytochemical properties of Azolla microphylla.Methods:Dried and powdered leaves (50g) were extracted with 300 ml of methanol using soxhlet extractor for 16 h at a temperature not exceeding the boiling point of the solvent. Standard methods have been used to screen the phytochemical constituents. Conclusions: From the observations it can be concluded that the plant extracts show the presence of several bioactive compounds which could be exploited further.

  9. USO DEL GÉNERO AZOLLA COMO BIOFERTILIZANTE EN EL CULTIVO DEL ARROZ (Oryza sativa L.

    Directory of Open Access Journals (Sweden)

    R. Castro

    2002-01-01

    Full Text Available Con el objetivo de evaluar los beneficios que proporciona la asociación arroz-Azolla al cultivo del arroz en Cuba, se desarrollaron estos trabajos durante el período comprendido entre marzo y julio de 1998, en la Estación Experimental del Arroz "Los Palacios", ubicada en el municipio Los Palacios en la provincia de Pinar del Río, sobre un suelo Hidromórfico Gley Nodular Ferruginoso Petroférrico. En el trabajo se evalúo la influencia de Azolla sobre el cultivo del arroz. Los resultados mostraron que este cereal fue influido de forma positiva por el uso de Azolla en las variantes utilizadas, destacándose la asociación con el cultivo del arroz, lo que permitió incrementar el número de granos por panícula, panícula.m2 y, por consiguiente, un aumento significativo en los rendimientos. Además, se observó que la asociación de este helecho regula la temperatura y el pH del agua, así como disminuye las cantidades de plantas que no son objeto de cultivo en más de un 80 %. El uso de este helecho por lo antes expuesto brinda significativos beneficios económicos con respecto al tratamiento testigo de producción.

  10. Improvement Bio sorption of Heavy Metals from Industrial Wastewater Using Azolla

    International Nuclear Information System (INIS)

    This study aims to improve the removal process which is vital for some heavy metals and natural radionuclides from industrial wastewater by bio sorption using living organisms with rapid growth as a trial to increase the efficient use of those organisms in the removal process is vital for the toxic elements. Bio sorption of heavy metal (Copper, Manganese, Iron, Zinc, Lead and Strontium) from industrial waste water (contaminated) with six different time periods for Azolla growth. The results indicate that Azolla plant able to on the absorption of ions of heavy elements and Sr and was up to the maximum absorption of most of the elements at a concentration of 50% of polluted water + 50% fresh water so we recommend using the plant Azolla as bio sorbent in the disposal and collection of heavy metals and radionuclides from industrial waste water and deal with it safely to humans and the environment. The results obtained confirm the ability of the fern to grow and absorb ion of heavy metal when mixed with industrial waste water and other sources of polluted water and act as bio filter. The optimum conditions for maximum removal of heavy metals were also determined. Study was conducted on recycling municipal wastewaters for cultivation of Azollamicrophylla biomass, which is used for inoculation into paddy fields as N bio fertilizer and has other applications as green manure,animal feed and bio filter.

  11. Removal of methyl orange from aqueous solution by Azolla filicoloides: Synthesis of Fe3O4 nano-particles and its surface modification by the extracted pectin of Azolla

    Institute of Scientific and Technical Information of China (English)

    Roohan Rakhshaee; Masoud Giahi; Afshin Pourahmad

    2011-01-01

    The modified Fe3O4 nano-particles with the extracted pectin from the cell wall of Azolla filicoloides (FN-EP) can remove methyl orange as a water-soluble azo dye from waste water better than Azolla and the extracted pectin from Azolla (EPA), alone. It could be due to more crowding the main functional groups of uptake after binding pectin with nano-particles. Thermodynamic studies showed that adsorption equilibrium constant (KL) and maximum adsorption capacities (Qmax) were increased with decreasing temperature (exothermic). The maximum uptake capacity (Qmax) of dye by FN-EP in a batch reactor was 0.533, 0.498 and 0.446 mmol/g at 5, 25 and 50 ℃, respectively. The enthalpy change (AH) and entropy change (△S) were -15.31 kJ/mol and -0.02434 kJ/mol K, respectively.

  12. 无藻萍的RAPD分析及其在三膘组满江红种间关系研究中的应用%RAPD analysis of Anabaena-free Azolla and its application in the study of interspecific relationships within section Azolla

    Institute of Scientific and Technical Information of China (English)

    陈坚; 徐国忠; 郑伟文; 唐龙飞

    2003-01-01

    从满江红Azolla Lam.萍-藻共生体中提取DNA进行的RAPD系统分析通常忽视了满江红样品的异质性.本研究通过获得无藻的满江红,比较有藻萍、无藻萍和离体藻之间的RAPD指纹图谱.发现从有藻萍中提取DNA的扩增反应来源于萍藻双方DNA的共同影响.依引物和植物样本的不同,共生双方对扩增产物的贡献结果不同,说明了用无藻萍进行RAPD检测的重要性.对满江红三膘组5个种的11个无藻萍样本进行了RAPD分析,由9个引物产生的127个DNA多态片段用于计算样本间的Jaccard相似系数和UPGMA树状聚类图.结果表明:3个蕨状满江红A. filiculoides Lam.样本聚为一类(种内相似系数:0.456-0.702); 2个墨西哥满江红A. mexicana Presyl样本(相似系数:0.587)与2个小叶满江红A. microphylla Kaulfuss样本(相似系数:0.203)聚为一类;2个卡洲满江红A. caroliniana Willd.样本聚为一类(相似系数:0.368);但2个洋洲满江红A. rubra R. Brown样本明显分开(相似系数:0.190),其中日本来源的洋洲满江红与蕨状满江红聚合(相似系数:0.206).根据现有的RAPD数据,结合三膘组满江红同工酶、有性杂交等证据讨论了三膘组中:(1)将日本满江红与大洋洲满江红均认作是A. rubra的合理性问题;(2)蕨状满江红、墨西哥满江红、小叶满江红间的亲缘关系问题以及(3)卡洲满江红的划分归属问题.认为日本满江红与大洋洲满江红应属于两个种,蕨状满江红是一个独立的种,而墨西哥满江红与小叶满江红间的遗传关系很近,不能分为不同的种,卡洲满江红的分类地位尚不能确定.

  13. Las plantas acuáticas en un contexto de aprovechamiento sostenible de los recursos naturales.I. Azolla spp.

    OpenAIRE

    Suárez, Jesus; González-García, Eliel

    1998-01-01

    Las plantas acuáticas son conceptuadas generalmente como malezas; sin embargo, en ocasiones se les da una utilización económica como alimento animal, fertilizante y para la descontaminación de efluentes. Ello hace que en la literatura aparezca esporádicamente información vinculada con estos usos. Por tal razón, en este artículo se intenta resumir una parte importante de la información referente a las especies del género Azolla. La Azolla, junto al Jacinto de Agua (Eichhornia crassipes),...

  14. Pengaruh Waktu Aplikasi Dan Pemberian Berbagai Dosis Kompos Azolla Terhadap Pertumbuhan Dan Produksi Tanaman Kedelai (Glycine Max (L) Merrill)

    OpenAIRE

    Wibowo, Ary

    2011-01-01

    Therefore, a research had been conducted at experimental field of College of Agriculture USU (± 25 m asl.) in March until July 2010 using factorial randomized block design with two factor, i.e. time application of azolla compost (21, 14, 7 day before planted and when planted) and variant doses of azolla compost (0, 2 0, 40, 60 g per plant). Parameters measured were plant’s height, numbers of leaf, numbers productive branch, shoot wet weight, shoot dry weight, root wet weight, root dry weight...

  15. Ability of the aquatic fern Azolla to remove chemical oxygen demand and polyphenols from olive mill wastewater

    Directory of Open Access Journals (Sweden)

    Sacchi, Angelo

    2007-03-01

    Full Text Available We investigated the biofiltration ability of the aquatic fern Azolla to remove polyphenols and chemical oxygen demand (COD from olive mill wastewater (OMWw collected from the traditional (TS and continuous (CS extraction systems. Azolla biomass was packed into five sequential Imhoff cones and five sequential columns. In both experiments, the filtrates collected from the 5th biofilter showed a decrease in polyphenol contents: from 7650 mg l–1 to 3610 mg l–1 in TS OMWw and from 3852 mg l–1 to 1351 mg l–1 in CS OMWw. The COD contents decreased from 110200 mg L–1 to 52400 mg L–1 in TS OMWw and from 41600 mg L–1 to 2300 mg L–1 in CS OMWw. A 5:1 OMWw to Azolla-fresh-weight ratio was optimal for both polyphenol and COD removal. The biofiltration ability of alfalfa was compared with that of Azolla, but the treatment with alfalfa did not result in the reduction of COD or polyphenols.La eficacia del helecho de agua azolla para eliminar polifenoles y reducir la demanda química de oxígeno (DQO de los alpechines obtenidos en el proceso de obtención tradicional y continuo del aceite de oliva, fue investigado mediante ensayos de filtración. Cinco conos secuenciales de Imhoff y cinco columnas secuenciales se rellenaron de biomasa de Azolla. En ambos experimentos, el filtrado procedente de la quinta extracción mostró una disminución en el contenido de polifenoles de 7650 mg L–1 a 3610 mg L–1en el alpechín obtenido mediante el sistema tradicional y de 3852 mg L–1 a 1351 mg L–1en el alpechín del sistema continuo. La demanda química de oxígeno del alpechín del sistema tradicional disminuyó de 110200 mg L–1 a 52400 mg L–1 en y de 41600 mg L–1a 2300 mg L–1en el procedente del sistema continuo. Una proporción en peso 5:1 de alpechín: Azolla fue la óptima tanto para la reducción de los polifenoles como para la de la DQO. La eficiencia del tratamiento biológico con alfalfa se comparó con la obtenida con Azolla. Los

  16. APLIKASI KOMBINASI KOMPOS JERAMI, KOMPOS AZOLLA DAN PUPUK HAYATI UNTUK MENINGKATKAN JUMLAH POPULASI BAKTERI PENAMBAT NITROGEN DAN PRODUKTIVITAS TANAMAN PADI BERRBASIS IPAT-BO

    Directory of Open Access Journals (Sweden)

    Ferina Rosiana

    2013-03-01

    Full Text Available Penelitian untuk mengetahui efek pemberian kombinasi kompos jerami dengan Azolla dan pupuk hayati majemuk terhadap peningkatan populasi bakteri penambat N dan produktivitas tanaman padi dengan teknologi IPAT-BO dilaksanakan dari bulan April hingga Juli 2012 di kebun percobaan Fakultas Pertanian, Universitas Padjadjaran, Jatinangor, dengan ketinggian + 740 m dpl. Penelitian ini menggunakan rancangan acak kelompok faktor tunggal dengan dua belas perlakuan dan tiga kali ulangan. Perlakuan terdiri dari (A tanpa kompos jerami, (B kompos jerami 2,5 ton ha-1, (C kompos jerami 5 ton ha-1, (D kompos Azolla 0,5 ton ha-1, (E kompos jerami 2,5 ton ha-1 + kompos Azolla 0,5 ton ha-1, (F kompos jerami 5 ton ha-1 + kompos Azolla 0,5 ton ha-1, (G pupuk hayati 400 g ha-1, (H kompos jerami 2,5 ton ha-1 + pupuk hayati 400 g ha-1, (I kompos jerami 5 ton ha-1 + pupuk hayati 400 g ha-1, (J kompos Azolla 0,5 ton ha-1 + pupuk hayati 400 g ha-1, (K kompos jerami 2,5 ton ha-1 + kompos Azolla 0,5 ton ha-1 + pupuk hayati 400 g ha-1, (L kompos jerami 5 ton ha-1 + kompos Azolla 0,5 ton ha-1 + pupuk hayati 400 g ha-1.Aplikasi perlakuan kompos jerami, kompos Azolla dan pupuk hayati majemuk memberikan pengaruh terhadap populasi penambat N (Azotobacter sp. dan Azospirilium sp. dan produktifitas tanaman padi. Aplikasi kompos jerami 2,5 ton ha-1 dengan pupuk hayati 400 g ha-1 memberikan hasil GKP yaitu 64,39 g tanaman-1 (6,13 ton ha-1. Kata kunci: IPAT-BO, kompos Azolla, kompos jerami, pupuk hayati.

  17. Factors regulating nitrogenase activity and hydrogen evolution in Azolla-Anabaena symbiosis

    Energy Technology Data Exchange (ETDEWEB)

    Banerjee, M.; Kumar, A.; Kumar, H.D. (Banaras Hindu Univ., Varanasi (India). Dept. of Botany)

    1989-01-01

    Nitrogenase activity and H{sub 2} production capacity have been studied in intact Azolla plants. Under aerobic conditions the plants showed a C{sub 2}H{sub 2} reduction rate of 6.65 nmoles C{sub 2}H{sub 4} mg {sup -1} fresh weight in light at 48 h. Considerable activity was also present in the dark. Though H{sub 2} evolution was detected under aerobic conditions there was multifold stimulation under anaerobic conditions. There was no significant change in nitrogenase activity under anaerobic conditions. Increasing concentrations of O{sub 2} inhibited nitrogenase activity but 5% O{sub 2} proved stimulatory for H{sub 2} evolution in light. In the dark, there was a gradual stimulation in H{sub 2} evolution even up to 20% O{sub 2}. The addition of combined nitrogen sources, namely NH{sub 4}Cl or KNO{sub 3} (10 mM), resulted in complete inhibition of the C{sub 2}H{sub 2}-reduction activity within 48 h, but H{sub 2} evolution was not inhibited. Indeed, these combined nitrogen sources stimulated H{sub 2} evolution. Though nitrogenase activity was affected, the heterocyst frequency remained unaltered. Phosphate addition resulted in significant stimulation of nitrogenase and H{sub 2} evolution activity. These results suggest that nitrogenase and H{sub 2} evolution activity in Azolla are affected by a number of factors which show a differential effect on nitrogenase and H{sub 2} evolution. Furthermore, our results indicate the presence of a soluble reversible hydrogenase in Azolla. (author).

  18. Azolla as an Economic Substitute to Soybean Based Feed for Poultry

    Directory of Open Access Journals (Sweden)

    Dev Raj Paudel

    2015-12-01

    Full Text Available A study on azolla as an economical substitute to soybean based poultry feed was conducted. The experiment consisted of four treatments laid out in Completely Randomized Design (CRD with five replications. Each experimental unit consisted of three birds. For this purpose, eight-day old, unsexed Hubbard broiler chicks were weighed and randomly assigned to each treatment. Four different levels of azolla meal were incorporated in the starter and finisher ration at a level of 0%, 10%, 15%, and 20% respectively. Body weight, weight gain, feed consumption, and feed efficiency were measured. The average cumulative weekly body weight for all weeks were found to be significantly different (P<0.05 for the various treatments. Maximum cumulative body weight (1360 g was found in broiler fed T2 ration in 5th week. Similar trend was also observed in 6th week showing highest cumulative weight (1882 g in control diet (T1 which was at par with T2 (1841 g. Significantly highest (420.3 g live weight gain was observed in T2 diet during 5th week. Overall mean of weekly feed consumption was recorded highest (784.33 g in T2 diet. The economic analysis of net income/kg live weight was the highest (Rs 18.86 in T1 which was at par with T2 (Rs 18.35. Likewise, cost of production/bird was highest (Rs 124.5 in T1 and lowest (Rs 112.2 in T4. The results show that azolla meal could be utilized to substitute up to 10% soybean meal from overall production and economic point of view.

  19. EFECTO DEL USO DE Azolla EN LOS RENDIMIENTOS DE CULTIVOS EN CONDICIONES DE ORGANOPÓNICOS

    OpenAIRE

    Castro, R; Maribel Rodríguez; Gloria E. Álvarez; M. Gil; R. Novo; R. I. Castro; Sandra H. Díaz

    2006-01-01

    En el período comprendido entre 1996 y 2001, se realizó el trabajo integrado por dos experimentos, en el organopónico del INRE, Los Palacios, Pinar del Río, con el objetivo de evaluar la influencia de la dosis de Azolla y el efecto residual sobre el rendimiento y la evolución del sustrato de los canteros de los organopónicos con aplicaciones periódicas de este helecho; para ello, se emplearon los cultivos de lechuga y cebollino. En el cultivo de la lechuga se evaluó el efecto de seis dosis de...

  20. The Contribution of Azolla and Urea in Lowland Rice Growth Production for Three Consecutive Seasons

    Directory of Open Access Journals (Sweden)

    EL. Sisworo

    2008-01-01

    Full Text Available Three field experiments have been carried out in three consecutive seasons namely wet season (120 days, dry season (120 days, wet season (120 days at Pusakanegara. The purpose of this experiment is to test whether urea combined with Azolla could increase lowland rice production and soil quality. The experimental plots have a size of 20 m2 and in each experimental plot an isotope plot was placed with a size of 1 m2. The isotope plots were used to apply labeled 15N urea. Treatments conducted were lowland varieties: Atomita I (V1 and IR-64 (V2; several levels of urea and Azolla : Pu1 = urea-tablets + an Azolla cover (Azc, Pu2 = urea-tablets + Azolla incorporated (Azi , Pu3 = urea-prill + Azc , Pu4 = urea-prill + Azi; seasons : Ss 1 = wet season, Ss2 = dry season, Ss3 = wet season. The experimental design used was a factorial experiment in a Randomized Block Design, where each treatment was replicated four times. Parameters used were, dry weight of straw (St, grain (G, plant (P1 = St + G in kg/ha; N-total percentage (% N-to of St and G, percentage N-derived from urea + Az (% N-Pu of St and G; percentage N-derived from soil (% N-S of St and G; uptake of N-Pu and N-S in St, G and P1. Some results of these experiment were, N-Pu play a less important role in growth of lowland crop expressed in several parameters compared to N-soil. The form of N-urea in tablets are superior to that the form of urea in prills. For the last product of lowland rice which is grain obviously V1 (Atomita-1 is better than V2 (IR-64 expressed in t/ha. The progress of seasons showed clearly that there is an N accumulation which might be the increase of soil organic matter (SOM and that means there is an increase in soil quality in the view point of N

  1. Evaluating the combined effects of pretilachlor and UV-B on two Azolla species.

    Science.gov (United States)

    Prasad, Sheo Mohan; Kumar, Sushil; Parihar, Parul; Singh, Anita; Singh, Rachana

    2016-03-01

    The present study assessed the comparative responses of two agronomic species of Azolla (A.microphylla and A. pinnata) exposed to man-made and natural stressors by evaluating biomass accumulation, pigments (chlorophyll a and b and carotenoid contents), photosynthetic activity and nitrogen metabolism. The study was carried out in field where two species of Azolla were cultured and treated with various concentrations (5, 10 and 20 μg ml(-1)) of herbicide; pretilachlor [2-chloro-2,6-diethyl-N-(2-propoxyethyl) acetanilide] and enhanced levels (UV-B1: ambient +2.2 kJ m(-2) day(-1) and UV-B2: ambient +4.4 kJ m(-2) day(-1)) of UV-B, alone as well as in combination. Biomass accumulation, photosynthetic pigments; chlorophyll a, b and carotenoids, photosynthetic oxygen yield and photosynthetic electron transport activities i.e. photosystem II (PS II) and photosystem I (PS I) in both the species declined with the increasing doses of pretilachlor and UV-B radiation, which further declined when applied in combination. The lower doses (5 and 10 μg ml(-1)) of pretilachlor and UV-B (UV-B1 and UV-B2) alone, damaged mainly the oxidation side of PS II, whereas higher dose (20 μg ml(-1)) of pretilachlor alone and in combination with UV-B1 and UV-B2 caused damage to PS II reaction centre and beyond this towards the reduction side. A significant enhancement in respiration was also noticed in fronds of both the Azolla species following pretilachlor and UV-B treatment, hence indicating strong damaging effect. The nitrate assimilating enzymes - nitrate reductase and nitrite reductase and ammonium assimilating enzymes - glutamine synthetase and glutamate synthase were also severely affected when treated either with pretilachlor and/or UV-B while glutamate dehydrogenase exhibited a stimulatory response. The study suggests that both the species of Azolla showed considerable damage under pretilachlor and UV-B treatments alone, however, in combination the effect was more intense. Further, in

  2. Growth and solar energy conversion of Azolla sp., cultivated under four solar irradiance flux density; Crescimento e conversao da energia solar de Azolla sp. cultivada em quatro densidades do fluxo radiante

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, E.F. de [Acre Univ., Rio Branco, AC (Brazil); Lopes, N.F. [Vicosa Univ., MG (Brazil). Dept. de Biologia Vegetal

    1994-02-01

    Growth and solar energy conversion were studied in three Azolla species grown under four levels (30, 50, 70 and 100%) of solar radiation incidence under outdoor conditions. Under full sunlight, the specie A. microphylla showed higher crop growth rate, relative growth rate, net assimilation rate and efficiency of solar energy conversion than the other ones. (author). 8 figs., 23 refs.

  3. Selectivity of three aquatic weeds as diet for Nile tilapia (Oreochromis niloticus)

    OpenAIRE

    Agbede, S.A.; Akinyemi, O.; A. E. Falaye; Atsanda, N. N.; Adeyemo, A.O.; Adesina, A. A.

    2001-01-01

    A total of sixty juvenile Oreochromis niloticus (Nile tilapia) were fed three species of aquatic weed, namely Azolla filiculoides (water fern), Elodea sp. and Pistia stratiotes (water lettuce) to determine which of the weeds will be selectively consumed, and preferred of all. A control group of twenty Nile tilapia was fed compounded feed. The selectivity of the weeds was observed based on their utilization as food source, and Azolla filiculoides was found to be highly utilized, followed by El...

  4. Pengaruh Pemberian Azolla Dan Urea Terhadap Pertumbuhan Dan Produksi Padi Sawah (Oryza sativa L.) Pada Inceptisol Di Cengkeh Turi Binjai

    OpenAIRE

    Nasution, Fatimah Azzah Rawani

    2012-01-01

    Penelitian bertujuan untuk mengetahui pemberian azolla dan urea terhadap pertumbuhan dan proruksi padi ( Oryza sativa L. ) pada Inceptisol kelurahan Cengkeh Turi Binjai. Padi merupakan bahan makanan utama, bahan makanan ini untuk memenuhi makanan pokok sebagian besar penduduk Indonesia, sehingga diperlukan peningkatan produksi bahan makanan dalam jumlah yang sesuai dengan peningkatan bahan makanan. Dengan peningkatan produksi yang tinggi harus diimbangi dengan penggunaan pupuk. 95030...

  5. Reconstruction of the Arctic Ocean environment during the Eocene Azolla interval using geochemical proxies and climate modeling. Geologica Ultraiectina (331)

    NARCIS (Netherlands)

    Speelman, E.N.

    2010-01-01

    With the realization that the Arctic Ocean was covered with enormous quantities of the aquatic floating fern Azolla 49 Myrs ago, new questions regarding the Eocene conditions facilitating these blooms arose. This dissertation describes the reconstruction of paleo-environmental conditions facilitatin

  6. Hepatoprotective and antioxidant effects of Azolla microphylla based gold nanoparticles against acetaminophen induced toxicity in a fresh water common carp fish (Cyprinus carpio L.

    Directory of Open Access Journals (Sweden)

    Selvaraj Kunjiappan

    2015-04-01

    Conclusion: Azolla microphylla phytochemically synthesized GNaP protects liver against oxidative damage and tissue damaging enzyme activities and could be used as an effective protector against acetaminophen-induced hepatic damage in fresh water common carp fish.

  7. Phytoremediation of domestic wastewaters in free water surface constructed wetlands using Azolla pinnata.

    Science.gov (United States)

    Akinbile, Christopher O; Ogunrinde, Temitope A; Che Bt Man, Hasfalina; Aziz, Hamidi Abdul

    2016-01-01

    Two constructed wetlands, one with Azolla pinnata plant (CW1) and the other without (CW2) for treating domestic wastewaters were developed. Fifteen water parameters which include: Dissolved Oxygen (DO), Biochemical Oxygen Demand (BOD5), Chemical Oxygen Demand (COD), Total Suspended Solid (TSS), Total Phosphorus (TP), Total Nitrogen (TN), Ammoniacal Nitrogen (NH3N), Turbidity, pH, Electrical Conductivity (EC), Iron (Fe), Magnesium (Mg), Manganese (Mn), and heavy metals such as Lead (Pb) and Zinc (Zn) were analyzed using standard laboratory procedures. The experiments were conducted in two (dry and wet) seasons simultaneously. Results showed considerable reductions in all parameters and metals including Zn in CW1 compared with CW2 in the two seasons considered while Pb and Mn were not detected throughout the study. Zn concentration levels reduced significantly in both seasons just as removal efficiencies of 70.03% and 64.51% were recorded for CW1 while 35.17% and 33.45% were recorded for CW2 in both seasons. There were no significant differences in the removal efficiencies of Fe in both seasons as 99.55%, 59.09%, 88.89%, and 53.56% were recorded in CW1 and CW2 respectively. Azolla pinnata has proved effective in domestic wastewater phytoremediation studies.

  8. Performance of Azolla caroliniana Willd. and Salvinia auriculata Aubl. on fish farming effluent.

    Science.gov (United States)

    Toledo, J J; Penha, J

    2011-02-01

    The increasing release of untreated fish farming effluents into water courses that flow to the Pantanal wetlands in Mato Grosso (Brazil) may drive this ecosystem to eutrophication. Therefore, the growth of Azolla caroliniana Willd. and Salvinia auriculata Aubl. in fish farming effluent and their effect on its quality were evaluated for 48 days in a greenhouse. The results were compared to those obtained in a nutrient rich solution (Hoagland ½ medium). Azolla caroliniana showed lower relative growth rate in fish farming effluent (0.020 d-1) than in Hoagland ½ medium (0.029 d-1). However, S. auriculata grew slightly better in fish farming effluent (0.030 d-1) than in Hoagland ½ medium (0.025 d-1). The species apparently contributed to reduce nitrate and phosphate concentration in Hoagland ½ medium. However, in fish farming effluent, only electrical conductivity and pH were reduced by plants compared to the control without plants. Thus, A. caroliniana and S. auriculata show low potential for improving effluent quality. PMID:21437397

  9. Cultivation of Azolla microphylla biomass on secondary-treated Delhi municipal effluents

    Energy Technology Data Exchange (ETDEWEB)

    Arora, A.; Saxena, S. [Indian Agricultural Research Institute, New Delhi (India). Centre for Conservation of Blue Green Algae

    2005-07-01

    Study was conducted on recycling municipal wastewaters for cultivation of Azolla microphylla biomass, which is used for inoculation into paddy fields as N biofertiliser and has other applications as green manure, animal feed and biofilter. Secondary-treated municipal wastewaters were collected from Wazirabad sewage treatment plant in New Delhi during all four seasons and tested for reactive P and heavy metal content. The reactive P levels in effluents ranged between 1-2 ppm and levels of heavy metals like Cd, Pb, Ni, Zn, Fe and Mn were well below permissible limits. A. microphylla was grown in sewage effluents and its dilutions prepared with tapwater. It showed good growth potential on sewage effluents. Doubling times during September and December months compared well with those on Espinase and Watanabe (E and W) medium and tapwater. Dried Azolla biomass produced on sewage waters did not show presence of toxic heavy metals Cd, Cr and Pb. However, levels of P in dried biomass cultivated on sewage effluents were lower as compared to those from E and W medium and tapwater. The biomass produced can be used for inoculating paddy fields or for other applications and polished wastewaters can be recycled for irrigation purposes. (author)

  10. Phytoremediation of domestic wastewaters in free water surface constructed wetlands using Azolla pinnata.

    Science.gov (United States)

    Akinbile, Christopher O; Ogunrinde, Temitope A; Che Bt Man, Hasfalina; Aziz, Hamidi Abdul

    2016-01-01

    Two constructed wetlands, one with Azolla pinnata plant (CW1) and the other without (CW2) for treating domestic wastewaters were developed. Fifteen water parameters which include: Dissolved Oxygen (DO), Biochemical Oxygen Demand (BOD5), Chemical Oxygen Demand (COD), Total Suspended Solid (TSS), Total Phosphorus (TP), Total Nitrogen (TN), Ammoniacal Nitrogen (NH3N), Turbidity, pH, Electrical Conductivity (EC), Iron (Fe), Magnesium (Mg), Manganese (Mn), and heavy metals such as Lead (Pb) and Zinc (Zn) were analyzed using standard laboratory procedures. The experiments were conducted in two (dry and wet) seasons simultaneously. Results showed considerable reductions in all parameters and metals including Zn in CW1 compared with CW2 in the two seasons considered while Pb and Mn were not detected throughout the study. Zn concentration levels reduced significantly in both seasons just as removal efficiencies of 70.03% and 64.51% were recorded for CW1 while 35.17% and 33.45% were recorded for CW2 in both seasons. There were no significant differences in the removal efficiencies of Fe in both seasons as 99.55%, 59.09%, 88.89%, and 53.56% were recorded in CW1 and CW2 respectively. Azolla pinnata has proved effective in domestic wastewater phytoremediation studies. PMID:26121232

  11. Inhibition of nitrogen-fixing activity of the cyanobiont affects the localization of glutamine synthetase in hair cells of Azolla.

    Science.gov (United States)

    Uheda, Eiji; Maejima, Kazuhiro

    2009-10-15

    In the Azolla-Anabaena association, the host plant Azolla efficiently incorporates and assimilates ammonium ions that are released from the nitrogen-fixing cyanobiont, probably via glutamine synthetase (GS; EC 6.3.1.2) in hair cells, which are specialized cells protruding into the leaf cavity. In order to clarify the regulatory mechanism underlying ammonium assimilation in the Azolla-Anabaena association, Azolla plants were grown under an argon environment (Ar), in which the nitrogen-fixing activity of the cyanobiont was inhibited specifically and completely. The localization of GS in hair cells was determined by immunoelectron microscopy and quantitative analysis of immunogold labeling. Azolla plants grew healthily under Ar when nitrogen sources, such as NO(3)(-) and NH(4)(+), were provided in the growth medium. Both the number of cyanobacterial cells per leaf and the heterocyst frequency of the plants under Ar were similar to those of plants in a nitrogen environment (N(2)). In hair cells of plants grown under Ar, regardless of the type of nitrogen source provided, only weak labeling of GS was observed in the cytoplasm and in chloroplasts. In contrast, in hair cells of plants grown under N(2), abundant labeling of GS was observed in both sites. These findings indicate that specific inhibition of the nitrogen-fixing activity of the cyanobiont affects the localization of GS isoenzymes. Ammonium fixed and released by the cyanobiont could stimulate GS synthesis in hair cells. Simultaneously, the abundant GS, probably GS1, in these cells, could assimilate ammonium rapidly. PMID:19464754

  12. Effect of partial supplementation of sun-dried Azolla as a protein source on the immunity and antioxidant status of commercial broilers

    Directory of Open Access Journals (Sweden)

    Biswal Chichilichi

    2015-09-01

    Full Text Available Aim: The present study was conducted to evaluate the effect of partial supplementation of sun-dried Azolla as a protein source on the immunity of commercial broilers in coastal Odisha. Materials and Methods: A 180 day-old broiler chicks were distributed in six dietary treatments viz. C1: Basal diet, C2: Basal diet + enzyme, T1: Basal diet +5% protein from Azolla, T2: Basal diet + 5% protein from Azolla + enzyme, T3: Basal diet +10% protein from Azolla, and T4: Basal diet + 10% protein from Azolla + enzyme. Cutaneous basophilc hypersensitivity (CBH and humoral immunity response were determined at the 38th day of age. At 42nd day, the weight of lymphoid organs, an antioxidant enzyme, and lipid peroxidation activity were determined. Results: The CBH response did not differ significantly among the treated groups, but the sheep red blood cells response was significantly higher in T4. The weight of lymphoid organs or immune organs of all the treated groups did not differ significantly (p>0.05. The erythrocyte catalase level of T4 group was found to be significantly higher than rest of the treated groups except T3. Conclusion: It may be concluded that supplementation of Azolla at 10% of dietary protein requirement along with enzyme supplementation in an isonitrogenous diet showed a better immune response in broilers.

  13. Ecotoxicidade de herbicidas para a macrófita aquática (Azolla caroliniana Ecotoxicity of herbicides for the aquatic macrophyte (Azolla caroliniana

    Directory of Open Access Journals (Sweden)

    A.F. Silva

    2012-09-01

    Full Text Available Os objetivos deste estudo foram avaliar Azolla caroliniana como planta-teste em estudos ecotoxicológicos e estimar a CL50;7d dos herbicidas 2,4-D, glyphosate, clomazone e oxyfluorfen. As plantas foram aclimatadas em sala de bioensaio. Para isso, foram selecionadas cinco plantas em 50 mL de meio de cultivo Hoagland. Após esse período, foram adicionados 50 mL de Hoagland mais o herbicida, completando o volume para 100 mL. A concentração letal de 50% (CL50;7d para A. caroliniana exposta ao herbicida 2,4-D foi de 708,35 mg L-1; ao glyphosate (formulação Scout®, de 23,66 mg L-1; ao glyphosate (formulação Trop®, de 38,91 mg L-1; ao clomazone, de 129,63 mg L-1; e ao oxyfluorfen, de 80,50 mg L-1. Os herbicidas glyphosate (Scout® e Trop® e oxyflourfen foram classificados como moderadamente tóxicos a A. caroliniana, e o clomazone e o 2,4-D, como praticamente não tóxicos. Conclui-se que A. caroliniana pode ser utilizada como planta bioindicadora de herbicidas à base de glyphosate e oxyfluorfen.The objectives of this study were to evaluate Azolla caroliniana as test plant in ecotoxicological studies and to estimate the LC50; 7d of the herbicides 2,4 D , glyphosate, and clomazone oxyfluorfen. The plants were acclimatized in the bioassay room. Five plants in 50 mL Hoagland culture medium were selected. After that, 50 mL of Hoagland and the herbicide were added, completing the volume to 100 mL. The 50% lethal concentration (LC50; 7d for A. caroliniana exposed to the herbicide 2,4- D, was 708.35 mg L-1; to glyphosate (formulation Scout®, 23.66 mg L-1; to glyphosate (formulation Trop®, 38.91 mg L-1; to clomazone, 129.63 mg L-1; and to oxyflourfen, 80.50 mg L-1. The herbicides glyphosate (Scout® and Trop® and oxyflourfen were classified as moderately toxic to A. caroliniana, while clomazone and 2,4-D were classified as practically non-toxic. It was concluded that A. caroliniana plants can be used as bio-indicators for glyphosate and

  14. Genetic Characterization of Old Grapevines collected in Oases of the Atacama Desert Caracterización Genética de Vides Antiguas colectadas en Oasis del Desierto de Atacama

    Directory of Open Access Journals (Sweden)

    Ingrid Poblete

    2011-09-01

    variantes génicas que pueden ser rescatadas para su uso directo o su incorporación en programas de fitomejoramiento. La primera etapa de este rescate es la colecta y caracterización del germoplasma propio de una región particular. Este estudio describe la caracterización genética de 21 accesiones de vid colectadas en el desierto de Atacama, en el Norte de Chile. La caracterización se basó en 12 marcadores de microsatélites (SSR, complementado con marcadores de tipo fragmentos amplificados de largo variable (AFLP. La mayoría de las accesiones colectadas con bayas negras corresponden al genotipo ‘País’, un cultivar antiguo que se encuentra distribuido en toda América. Sin embargo, entre las accesiones de baya negra, una de ellas mostró un fenotipo abortivo severo (22S7, y otro (6S4 difirió en un alelo con respecto a ‘País’. Ambos podrían ser ejemplos de mutaciones somáticas, aun cuando no fue posible detectar variaciones en sus patrones de AFLP. Por otra parte, la única accesión con bayas negras que exhibió características genéticas diferentes a ‘País’ correspondió a ‘Gros Colman’ (5CN, un genotipo supuestamente proveniente de Georgia, Rusia, introducido en esta región a mediados del siglo XX. Una mayor diversidad genética fue detectada entre las accesiones de bayas blancas y rosadas, que se clasificaron en cinco clados en base a sus alelos de microsatélites. De estos genotipos, 11Si se identificó como ‘Emperatriz’ o ‘Red Seedless’, una variedad de origen argentino, mientras que las accesiones 16H1 y 17H2 correspondieron a un cruzamiento de ‘Moscatel de Alejandría’ × ‘País’. Por último, la accesión 20S5 se identificó como ‘Ahmeur bou Ahmeur’, un genotipo argelino de bayas de color rosado. Dos genotipos semillados de bayas blancas, pequeñas o grandes, no se asimilaron con alguna variedad conocida. Se discute también la posibilidad de utilizar algunas de estas accesiones en fitomejoramiento, para aumentar

  15. Molecular mechanism for cadmium-induced anthocyanin accumulation in Azolla imbricata.

    Science.gov (United States)

    Dai, Ling-Peng; Dong, Xin-Jiao; Ma, Hai-Hu

    2012-04-01

    Anthocyanins inducibly synthesized by Cd treatment showed high antioxidant activity and might be involved in internal detoxification mechanisms of Azolla imbricata against Cd toxicity. In order to understand anthocyanin biosynthesis mechanism during Cd stress, the cDNAs encoding chalcone synthase (CHS) and dihydroflavonol reductase (DFR), two key enzymes in the anthocyanin synthesis pathway, were isolated from A. imbricata. Deduced amino acid sequences of the cDNAs showed high homology to the sequences from other plants. Expression of AiDFR, and to a lesser extent AiCHS, was significantly induced in Cd treatment plant in comparison with the control. CHS and DFR enzymatic activities showed similar pattern changes with these genes expression during Cd stress. These results strongly indicate that Cd induced anthocyanin accumulation is probably mediated by up-regulation of structural genes including CHS and DFR, which might further increase the activities of enzymes encoded by these structural genes that control the anthocyanin biosynthetic steps. PMID:22225708

  16. Phytoremediation of heavy metals from fly ash pond by Azolla caroliniana.

    Science.gov (United States)

    Pandey, Vimal Chandra

    2012-08-01

    Abundance of naturally growing Azolla caroliniana (water fern) on the surface of metal enriched fly ash (FA) pond reflects its toxitolerant characteristics. Results indicate the efficiency of A. caroliniana for phytoremediation of FA pond because of its higher bioconcentration factor. The metal concentration ranged from 175 to 538 and 86 to 753mgkg(-1) in roots and fronds, respectively. Bioconcentration factor (BCF) values of all metals in root and frond ranged from 1.7 to18.6 and 1.8 to 11.0, respectively, which were greater than one and indicates the metal accumulation potential of A. caroliniana. Translocation factor (TF) ranged from 0.37 to 1.4 for various heavy metals. The field result proved that A. caroliniana is a potential accumulator for the examined heavy metals and can be used for phytoremediation of FA pond. PMID:22677365

  17. Antimicrobial activity and identification of potential antimicrobial compounds from aquatic pteridophyte, Azolla microphylla Kaulf.

    Science.gov (United States)

    Abraham, G; Yadav, R K; Kaushik, G K

    2015-04-01

    Azolla microphylla Kaulf. is an aquatic nitrogen fixing pteridophyte commonly found in aquatic habitats including paddy fields. Methanolic extract of the fronds of A. microphylla was subjected to partial purification by solvent partitioning with diethyl ether and ethyl acetate followed by hydrolysis, and further partitioning with ethyl acetate. The two fractions, thus obtained were tested for antibacterial activity. It was observed that the ethyl acetate fraction inhibited the growth of the pathogenic bacterium Xanthomonas oryzae. The GC-MS analysis of the ethyl acetate fraction showed several prominent peaks with retention time ranging from 8.83 to 45.54 min. A comparison of these peaks with the GC-MS libraries revealed that it could be eicosenes and heptadecanes with potential of antimicrobial activity. PMID:26011985

  18. Influence of an azolla layer on growth, production and efficiency and use of 15N Labelled urea fertilizer of lowland rice

    International Nuclear Information System (INIS)

    The purpose of this experiment was to study the effect of an Azolla mat on rice growth, percentage of N-total, N derived from fertilizer and urea efficiency. The experiment used a factorial design which consists of 2 factors : the using of an Azolla mat and the doses of N fertilizer. The first factor comprised 2 levels, with (A1) and without an azolla mat (A0). The second factor comprised 4 levels, 0(N0), 30 (N1), 60(N2) and 90 kg N/ha(N3).Application of 15N labeled Urea done to determine percentage N-derived from Urea efficiency , which was divided in 2 experiment series as follows : application Urea at planting (experiment I) and one month after planting (experiment II). The experiments were carried out in the green house of Agricultural Faculty of IPB and continued in the laboratory of BATAN. Parameters observed were plant height, number of tillers, number of panicles, number of grains/panicle, dry weight of grain, straw and percentage of empty grain. Result of the experiment showed that an Azolla mat, the doses of N fertilizer and interaction of both increased all the parameters observed yield, except for percentage of empathy grain, up to the dose of N fertilizer at 60 kg N/ha and decreased at the dose of N fertilizer at 90 kg N/ha. The treatment of Urea fertilizer doses resulted in a significant difference on the percentage of N- derived from Urea for both experiment, but not for the treatment of an Azolla mat and the interaction of both treatment. An azolla mat, N fertilizer and interaction of both did not result in a significantly difference on the percentage of total-N and Urea efficiency. The best treatment combination was treatment of an azolla mat and dose of Urea fertilizer of 60 kg N/ha. (author)

  19. Production of biogas from Azolla pinnata R. Br. and Lemna minor L. : effect of heavy metal contamination

    Energy Technology Data Exchange (ETDEWEB)

    Jain, S.K.; Gujral, G.S.; Jha, N.K.; Vasudevan, P. (Indian Inst. of Tech., New Delhi (India))

    1992-01-01

    The absorption of iron, copper, cadmium, nickel, lead, zinc, manganese and cobalt by Azolla pinnata R.Br and Lemna minor L., and subsequent utilization of this biomass for production of biogas (methane), have been investigated. Iron or manganese did not have any toxic effect on the anaerobic fermentation of Azolla and Lemna, while copper, cobalt, lead and zinc showed toxicity. At low concentrations cadmium and nickel showed a favourable effect on the rate of biogas production and its methane content, but with increase in concentrations,rate of biogas production and methane content decreased. However, although there was this decrease in biogas production and methane content, the methane content of biogas was still higher than that which was obtained from non-contaminated biomass. (author).

  20. Combined use of Azolla and loach suppressed paddy weeds and increased organic rice yield: second season results

    OpenAIRE

    Weiguo Cheng; Miwa Takei; Valensi Kautsar; Yuka Sasaki; Satoru Sato; Keitaro Tawaraya; Hironori Yasuda

    2015-01-01

    Organic farming uses alternatives to agricultural chemicals such as synthetic fertilizers and pesticides. The primary challenge in organic rice farming is controlling weeds without using herbicides and improving rice yield without chemical fertilizers. In our previous paper entitled as combined use of Azolla and loach suppressed weed Monochoria vaginalis and increased rice yield without agrochemicals, we reported the first year rice growth season results from an in situ container experiment. ...

  1. Photoproduction of hydrogen by a non-sulphur bacterium isolated from root zones of water fern Azolla pinnata

    Energy Technology Data Exchange (ETDEWEB)

    Singh, S.P.; Srivastava, S.C.; Pandey, K.D. (Banaras Hindu Univ., Varanasi (IN). Centre of Advanced Study in Botany)

    1990-01-01

    A photosynthetic bacterium Rhodopseudomonas sp. BHU strain 1 was isolated from the root zone of water fern Azolla pinnata. The bacterium was found to produce hydrogen with potato starch under phototrophic conditions. The immobilized bacterial cells showed sustained hydrogen production with a more than 4-fold difference over free cell suspensions. The data have been discussed in the light of possible utilization of relatively cheaper raw materials by non-sulphur bacteria to evolve hydrogen. (author).

  2. Pemberian Azolla Uutuk Mengurangi Dosis Urea Padi Sawah (Oryza sativa L ) Pada Inseptisol di Silakkidir Kecamatan Hutabayuraja Kabupaten Simalungun

    OpenAIRE

    Purba, Freddy Alfian

    2012-01-01

    Penelitian ini bertujuan untuk melihat pengaruh Azolla dalam menyuplai N pada pertanaman padi sawah {Oryza sativa L ) pada Inseptisol di Silakkidir, Kecamatan Hutabayuraja Simalungun.. Pelaksanaan penelitian ini dilakukan di Desa Silakkidir Kecamatan Hutabayuraja Kabupaten Simalungun. Penelitian ini dimulai dari bulan Juni sampai November 2003. Rancangan yang digunakan adalah rancangan acak kelompok (RAK) Non Faktonal dengan 5 perlakuan dan 3 ulangan, masing- masing perlakuan adalah : F...

  3. Study on the Method of Extracting LPC of Azolla%红萍LPC提取方法的研究

    Institute of Scientific and Technical Information of China (English)

    杨有泉; 姜传京; 应朝阳; 陆烝; 陈敏; 邓素芳

    2013-01-01

    In order to A.caroliniana Wild.3001 as representative,based on the method of direct heating,acid extraction,alkali extraction,acidification heating method,alkali heating method comparison of 5 methods,Determine the acidification heating method for the optimal extraction method for Azolla LPC.Acidification heating extraction of Azolla LPC optimal conditions for:solid-liquid ratio 1∶3,beating time 4 min,heating temperature 65 ℃,heating time 7 min,pH 3; Effects of Azolla LPC extraction rate of primary and secondary factors of heating temperature>pH>solid-liquid ratio>beating time>heating time.By adopting the process conditions are Azolla LPC extraction rate was 33.28%,obtaining rate was 1.34%.%以卡州萍3001 (A.caroliniana Wild.3001)为代表,通过比较直接加热法、酸提法、碱提法、酸化加热法、碱化加热法5种方法,确定酸化加热法为红萍LPC提取的最佳方法.酸化加热法提取红萍LPC的最佳工艺条件是:料液比1∶3,打浆时间4 min,加热温度65℃,加热时间7 min,pH 3.0.影响红萍LPC提取率的主次因素为加热温度>pH值>料液比>打浆时间>加热时间.采用上述工艺条件得到的红萍LPC的提取率为33.28%,得率1.34%.

  4. Effect of Azolla Based - Organic Fertilizer, Rock Phosphate and Rice Hull Ash on Rice Yield and Chemical Properties of Alfisols

    OpenAIRE

    Sudadi; Sumarno; Wiki Handi

    2014-01-01

    The application of chemical fertilizer for long time may adverse soil environment. Organic agriculture, for example combination use of azolla based-organic fertilizer, phosphate rock and rice hull ash, was one of ways that able to recover it. Research was conducted in Sukosari, Jumantono, Karanganyar while soi chemical properties analysis was analysed in Soil Chemistry and Fertility Laboratory, Fac. of Agriculture, Sebelas Maret University April to November 2013. Research design used was R...

  5. Pengaruh Pemupukan N, P, K Dengan Menggunakan Azolla,Fosfat Alam Dan Arang Jerami Padi Pada Tanaman Padi Sawah

    OpenAIRE

    Taofiq, Muhammad

    2012-01-01

    The research was combined by using the non factorial Randomized Block Design in with 9 treatments and 3 replications so that it was obtained 27 experimental units. Each treatment was combined between nature fertilizers (Azolla, Rock Phosphate, and Rice Straw Charcoal) and anorganic fertilizers (Urea, TSP, and KC1). The rest Jts showed that the combination between nature fertilizers with anorganic fertilizers had the affect significantly available soil phosphate, exchangeable soil potassium...

  6. Azolla-Anabaena as a Biofertilizer for Rice Paddy Fields in the Po Valley, a Temperate Rice Area in Northern Italy

    Directory of Open Access Journals (Sweden)

    Stefano Bocchi

    2010-01-01

    Full Text Available Azolla is a floating pteridophyte, which contains as endosymbiont the nitrogen-fixing cyanobacterium Anabaena azollae (Nostocaceae family. Widely cultivated in the Asian regions, Azolla is either incorporated into the soil before rice transplanting or grown as a dual crop along with rice. To examine the feasibility of its use in flooded rice fields sited in the Temperate European Areas, we carried out a series of experiments in PVC tanks during 2000–2002 in Po Valley (northern Italy conditions, to study the growth-development dynamics and the resistance/tolerance to low temperatures and to commonly used herbicides of several different Azolla strains. Three out of five strains tested survived the winter, with an increase in biomass from March to May producing approximately 30–40 kg ha−1 of nitrogen. One of these strains, named “Milan”, emerged as the most resistant to herbicide and the most productive. Of the herbicides tested, Propanil permitted the survival of growing Azolla.

  7. Effect of feeding different levels of Azolla pinnata on blood biochemicals, hematology and immunocompetence traits of Chabro chicken

    Directory of Open Access Journals (Sweden)

    Deepesh Bharat Mishra

    2016-02-01

    Full Text Available Aim: The present study was conducted to see the effect of feeding different levels of Azolla meal on blood biochemicals, hematology and immunocompetence traits of Chabro chicken. Materials and Methods: The study was conducted on 160 Chabro chicks, which were randomly divided into four treatment groups each with four replicates of 10 birds. The first treatment (T1 served as a control in which basal diets was offered without Azolla supplementation while in T2, T3, and T4 groups, basal diet was replaced with Azolla meal at 5%, 7.5%, and 10% levels, respectively. A feeding trial was conducted upto 8 weeks. At the last week of trial, blood samples were collected randomly from one bird of each replicate and plasma was separated to estimate certain biochemical parameters, some blood metabolites, minerals and enzymes like alanine aminotransferase and aspartate aminotransferase (AST. Hematological parameters such as hemoglobin, packed cell volume, total leukocytes count and differential leukocytes count were estimated in fresh blood just after collection. The humoral immune response was measured against sheep red blood cells,and cell-mediated immune response was measured against phyto hemagglutinin lectin from Phaseolus vulgaris (PHA-P. Results: The study showed that hematological profile of the Chabro bird was not affected by any treatment except heterophil and lymphocyte which was found higher in T2 and T3 groups and eosinophil was found higher in a T3 group than control. Blood glucose, creatinine, cholesterol, total protein, albumin, uric acid, and triglycerides were found similar in all the groups and within the normal values for broiler chicken. Liver enzymes and macro mineral content in blood were found similar in all the treatment groups and within normal physiological range. Although AST was found higher in 10% replacement group than control, the value was within normal range for broiler chicken. Although antibody titer was found similar in all

  8. Sea surface salinity of the Eocene Arctic Azolla event using innovative isotope modeling

    Science.gov (United States)

    Speelman, E. N.; Sewall, J. O.; Noone, D.; Huber, M.; Sinninghe Damste, J. S.; Reichart, G. J.

    2009-04-01

    With the realization that the Eocene Arctic Ocean was covered with enormous quantities of the free floating freshwater fern Azolla, new questions regarding Eocene conditions facilitating these blooms arose. Our present research focuses on constraining the actual salinity of, and water sources for, the Eocene Arctic basin through the application of stable water isotope tracers. Precipitation pathways potentially strongly affect the final isotopic composition of water entering the Arctic Basin. Therefore we use the Community Atmosphere Model (CAM3), developed by NCAR, combined with a recently developed integrated isotope tracer code to reconstruct the isotopic composition of global Eocene precipitation and run-off patterns. We further addressed the sensitivity of the modeled hydrological cycle to changes in boundary conditions, such as pCO2, sea surface temperatures (SSTs) and sea ice formation. In this way it is possible to assess the effect of uncertainties in proxy estimates of these parameters. Overall, results of all runs with Eocene boundary conditions, including Eocene topography, bathymetry, vegetation patterns, TEX86 derived SSTs and pCO2 estimates, show the presence of an intensified hydrological cycle with precipitation exceeding evaporation in the Arctic region. Enriched, precipitation weighted, isotopic values of around -120‰ are reported for the Arctic region. Combining new results obtained from compound specific isotope analyses (δD) on terrestrially derived n-alkanes extracted from Eocene sediments, and model outcomes make it possible to verify climate reconstructions for the middle Eocene Arctic. Furthermore, recently, characteristic long-chain mid-chain ω20 hydroxy wax constituents of Azolla were found in ACEX sediments. δD values of these C32 - C36 diols provide insight into the isotopic composition of the Eocene Arctic surface water. As the isotopic signature of the runoff entering the Arctic is modelled, and the final isotopic composition of

  9. Removal of

    OpenAIRE

    Roohan Rakhshaee; Zahra Zamiraee; Somaieh Baghipour; Mohammad Panahandeh

    2013-01-01

    Background and Objectives: Azolla Filiculoides as a non-living fern was used in a batch system to remove "Basic Blue 3", which is a cationic dye and a carcinogenic agent.Materials and Methods: We used a batch system by applying certain concentrations of dye contaminant and in the presence of a certain amount of adsorbent under optimum conditions. The main groups presenting in the Azolla cell wall were evaluated by acidification and alkalization of Azolla's media and then potentiometric titrat...

  10. Identification of a common cyanobacterial symbiont associated with Azolla spp. through molecular and morphological characterization of free-living and symbiotic cyanobacteria.

    OpenAIRE

    Gebhardt, J S; Nierzwicki-Bauer, S A

    1991-01-01

    Symbiotically associated cyanobacteria from Azolla mexicana and Azolla pinnata were isolated and cultured in a free-living state. Morphological analyses revealed differences between the free-living isolates and their symbiotic counterparts, as did restriction fragment length polymorphism (RFLP) analyses with both single-copy glnA and rbcS gene probes and a multicopy psbA gene probe. RFLP analyses with Anabaena sp. strain PCC 7120 nifD excision element probes, including an xisA gene probe, det...

  11. 铀在植物-微生物共生体系满江红体内的分布%Distribution of Uranium in the Plant_microbe Symbiotic System_Azolla Imbircata

    Institute of Scientific and Technical Information of China (English)

    胡南; 丁德馨; 潘长春; 胡劲松; 李乐; 李广悦; 王永东; 郑济芳

    2014-01-01

    采用扫描电镜( SEM)和能谱( EDS)联用分析技术,研究了铀在植物—微生物共生体系满江红中的分布,结果发现,铀在蕨类植物满江红和微生物满江红鱼腥藻中均有分布,满江红鱼腥藻中铀含量略高于蕨类植物满江红,表明了蕨类植物满江红和微生物满江红鱼腥藻都参与了去除水体中铀的过程。%The distribution of uranium in the plant_microbe symbiotic system_Azolla imbir_cata was analyzed by scanning electron microscope( SEM) and energy dispersive spectrom_eter( EDS ) hyphenated technique. The results showed that uranium distributed in both fern_Azolla imbricata and microbe_Anabena azollae. The content of uranium in Anabena azollae was slightly higher than that in Azolla imbricata. The fern_Azolla imbricata and mi_crobe_Anabena azollae had a synergistic effect on the process of removing uranium from the water.

  12. 小叶满江红(Azolla microphylla)内生细菌多样性的T-RFLP分析%T-RFLP Analysis on Diversity of Endophytic Bacteria in Azolla microphylla

    Institute of Scientific and Technical Information of China (English)

    郑斯平; 陈彬; 王瑾; 陆培基

    2012-01-01

    [目的]应用T-RFLP技术揭示小叶满江红内生细菌的多样性.[方法]将分离自实验室无菌培养的小叶满江红内生细菌进行16SrDNA-PCR扩增,产物用MspⅠ、HhaⅠ、HaeⅢ、HinfⅠ4种内切酶进行酶切,通过检测带荧光标记的片段,将所获得的数据用相关的软件工具Phylogenetic Assignment Tool(PAT)进行比对分析.[结果]通过比对获得10个科42个属的细菌菌种.[结论]T-RFLP技术配合生物信息学软件能较有效地揭示小叶满江红内生细菌的多样性.%[ Objective ] To explore the diversity of endophytic bacteria in Azolla microphylla by using T-RFLP. [ Method ] PCR products of DNA of endophytic bacteria in Azolla microphylla which were cultured by our laboratory in sterile conditions were digested with restriction endoenzyme Mspl, Hhal, Haelll, Hind, respectively. The terminal fragment was analyzed with fluorescent through Phylogenetic Assignment Tool (PAT). [ Result ] Bacteria belong to 10 Phylums 42 Classes were gained after analysis. [ Conclusion ] The genetic diversity of endophytic bacteria in Azolla microphylla could be figured out by using T-RFLP and bioinformatic software.

  13. Induction of salt tolerance in Azolla microphylla Kaulf through modulation of antioxidant enzymes and ion transport.

    Science.gov (United States)

    Abraham, Gerard; Dhar, Dolly Wattal

    2010-09-01

    Azolla microphylla plants exposed directly to NaCl (13 dsm(-1)) did not survive the salinity treatment beyond a period of one day, whereas plants exposed directly to 4 and 9 dsm(-1) NaCl were able to grow and produce biomass. However, plants pre-exposed to NaCl (2 dsm(-1)) for 7 days on subsequent exposure to 13 dsm(-1) NaCl were able to grow and produce biomass although at a slow rate and are hereinafter designated as pre-exposed plants. The pre-exposed and directly exposed plants distinctly differed in their response to salt in terms of lipid peroxidation, proline accumulation, activity of antioxidant enzymes, such as SOD, APX, and CAT, and Na(+)/K(+) ratio. Efficient modulation of antioxidant enzymes coupled with regulation of ion transport play an important role in the induction of salt tolerance. Results show that it is possible to induce salt adaptation in A. microphylla by pre-exposing them to low concentrations of NaCl. PMID:20422236

  14. Effects of lead accumulation on the Azolla caroliniana-Anabaena association.

    Science.gov (United States)

    Roberts, Anne E; Boylen, Charles W; Nierzwicki-Bauer, Sandra A

    2014-04-01

    The effect of lead accumulation on photopigment production, mineral nutrition, and Anabaena vegetative cell size and heterocyst formation in Azolla caroliniana was investigated. Plants were exposed to 0, 1, 5, 10, and 20 mg L(-1) lead acetate for ten days. Lead accumulation increased when plants were treated with higher lead concentrations. Results revealed a statistically significant decline in total chlorophyll, chlorophyll a, chlorophyll b, and carotenoids in 5, 10, and 20 mg Pb L(-1) treatment groups as compared to plants with 0 or 1 mg Pb L(-1) treatments. No statistically significant change in anthocyanin production was observed. Calcium, magnesium, and zinc concentrations in plants decreased in increasing treatment groups, whereas sodium and potassium concentrations increased. Nitrogen and carbon were also found to decrease in plant tissue. Anabaena vegetative cells decreased in size and heterocyst frequency declined rapidly in a Pb dose-dependent manner. These results indicate that, while A. caroliniana removes lead from aqueous solution, the heavy metal causes physiological and biochemical changes by impairing photosynthesis, changing mineral nutrition, and impeding the growth and formation of heterocysts of the symbiotic cyanobacteria that live within leaf cavities of the fronds. PMID:24509077

  15. Uptake and toxicity of arsenic, copper, and silicon in Azolla caroliniana and Lemna minor.

    Science.gov (United States)

    Rofkar, Jordan R; Dwyer, Daryl F; Bobak, Deanna M

    2014-01-01

    Here we report on the analysis of two aquatic plant species, Azolla caroliniana and Lemna minor, with respect to tolerance and uptake of co-occurring arsenic, copper, and silicon for use in engineered wetlands. Plants were cultured in nutrient solution that was amended with arsenic (0 or 20 microM), copper (2 or 78 microM), and silicon (0 or 1.8 mM) either singly or in combination. We hypothesized that arsenic and copper would negatively affect the uptake of metals, growth, and pigmentation and that silicon would mitigate those stresses. Tolerance was assessed by measuring growth of biomass and concentrations of chlorophyll and anthocyanins. Both plant species accumulated arsenic, copper, and silicon; L. minor generally had higher levels on a per biomass basis. Arsenic negatively impacted A. caroliniana, causing a 30% decrease in biomass production and an increase in the concentration of anthocyanin. Copper negatively impacted L. minor, causing a 60% decrease in biomass production and a 45% decrease in chlorophyll content. Silicon augmented the impact of arsenic on biomass production in A. caroliniana but mitigated the effect of copper on L. minor. Our results suggest that mixtures of plant species may be needed to maximize uptake of multiple contaminants in engineered wetlands. PMID:24912207

  16. Uptake and toxicity of arsenic, copper, and silicon in Azolla caroliniana and Lemna minor.

    Science.gov (United States)

    Rofkar, Jordan R; Dwyer, Daryl F; Bobak, Deanna M

    2014-01-01

    Here we report on the analysis of two aquatic plant species, Azolla caroliniana and Lemna minor, with respect to tolerance and uptake of co-occurring arsenic, copper, and silicon for use in engineered wetlands. Plants were cultured in nutrient solution that was amended with arsenic (0 or 20 microM), copper (2 or 78 microM), and silicon (0 or 1.8 mM) either singly or in combination. We hypothesized that arsenic and copper would negatively affect the uptake of metals, growth, and pigmentation and that silicon would mitigate those stresses. Tolerance was assessed by measuring growth of biomass and concentrations of chlorophyll and anthocyanins. Both plant species accumulated arsenic, copper, and silicon; L. minor generally had higher levels on a per biomass basis. Arsenic negatively impacted A. caroliniana, causing a 30% decrease in biomass production and an increase in the concentration of anthocyanin. Copper negatively impacted L. minor, causing a 60% decrease in biomass production and a 45% decrease in chlorophyll content. Silicon augmented the impact of arsenic on biomass production in A. caroliniana but mitigated the effect of copper on L. minor. Our results suggest that mixtures of plant species may be needed to maximize uptake of multiple contaminants in engineered wetlands.

  17. Phytochemical studies on Azolla pinnata R. Br., Marsilea minuta L. and Salvinia molesta Mitch

    Institute of Scientific and Technical Information of China (English)

    Johnson Marimuthu Antonisamy; Mony Mahesh; Zachariah Miller Paul; Solomon Jeeva

    2011-01-01

    Objective: To evaluate the phytochemical properties of Azolla pinnata R. Br., Marsilea minuta L. and Salvinia molesta Mitch. Methods: The dried and powered leaves materials (50 g) were extracted successively with 250 mL of petroleum ether, ethyl acetate, methanol, chloroform, acetone, benzene and water by using Soxhlet extractor for 8 h at a temperature not exceeding the boiling point of the solvent. Phytochemical screening of the extracts was carried out according to the standard methods. Results: Out of eighteen tested extracts, eighteen extracts showed the presence of phenolics. Next to that, fourteen extracts were illustrated their existence of tannin. Ten extracts showed the occurrence of carbohydrates in the crude extracts of the selected plants. Steroid and saponin are present in eight extracts, next to that xanthoprotein is present in six extracts, followed by flavonoid and protein which are present in five extracts. Carboxylic acid showed its presence only in two extracts. Conclusions: From the results, it can be concluded that the three plants extracts show the presence of many bioactive compounds after extensive investigation. We recommend further research on these plants to quantify the concentration of these compounds. Further work will accentuate the isolation and characterization of active principles responsible for bio-efficacy and bioactivity.

  18. Effects of lead accumulation on the Azolla caroliniana-Anabaena association.

    Science.gov (United States)

    Roberts, Anne E; Boylen, Charles W; Nierzwicki-Bauer, Sandra A

    2014-04-01

    The effect of lead accumulation on photopigment production, mineral nutrition, and Anabaena vegetative cell size and heterocyst formation in Azolla caroliniana was investigated. Plants were exposed to 0, 1, 5, 10, and 20 mg L(-1) lead acetate for ten days. Lead accumulation increased when plants were treated with higher lead concentrations. Results revealed a statistically significant decline in total chlorophyll, chlorophyll a, chlorophyll b, and carotenoids in 5, 10, and 20 mg Pb L(-1) treatment groups as compared to plants with 0 or 1 mg Pb L(-1) treatments. No statistically significant change in anthocyanin production was observed. Calcium, magnesium, and zinc concentrations in plants decreased in increasing treatment groups, whereas sodium and potassium concentrations increased. Nitrogen and carbon were also found to decrease in plant tissue. Anabaena vegetative cells decreased in size and heterocyst frequency declined rapidly in a Pb dose-dependent manner. These results indicate that, while A. caroliniana removes lead from aqueous solution, the heavy metal causes physiological and biochemical changes by impairing photosynthesis, changing mineral nutrition, and impeding the growth and formation of heterocysts of the symbiotic cyanobacteria that live within leaf cavities of the fronds.

  19. Biological Characteristic of Azolla imbricata (Roxb) Nak and Its Utilization in Fisheries%满江红Azolla imbricata ( Roxb.) Nak的生物学特征及其渔业利用

    Institute of Scientific and Technical Information of China (English)

    胡家文; 沈子伟; 陈小江; 姚维志

    2005-01-01

    满江红(Azolla imbricata)天然资源丰富,产量高,渔业利用价值比较大.论文综述了近年来满江红的部分研究工作,介绍了满江红的生物学特征及其渔业利用,在前人的基础上,提出利用"鱼类-满江红"走生态养殖的模式.

  20. Pengaruh Kombinasi Urea Dan Azolla pinnata Serta Waktu Aplikasinya Terhadap Pertumbuhan Dan Produksi Padi (Oryza Sativa, L)

    OpenAIRE

    Juanda, Boy Riza

    2013-01-01

    Urea is a chemically made nitrogen (N) source, and well being known by farmers. The intensive use of urea fertilizers in the huge amounts can damage soil, thus application of N organic source is required to fulfill N demand by plant and to reduce urea usage. The aim of this study was to (1). To determine the effect of applying combination of urea with Azolla on growth and yield of paddy, (2). To determine the effect application N source time on growth and yield of paddy, (3). To determin...

  1. 华北地区稻萍体系中几个问题的探讨%SOME ASPECTS OF RICE-AZOLLA ASSOCIATION IN NORTHERN CHINA

    Institute of Scientific and Technical Information of China (English)

    张荣菊; 宋未; 尤崇杓

    1986-01-01

    @@ 本工作观察了细绿萍(A2olla filiculoides Lam)孢子果的有性繁殖.华北地区在18-25℃和1000-15001x光照下,从孢子果发育成幼萍需要25-30天.应用16N稀释技术证明,细绿萍中的氮40-60%来自所固定的空气氮.红萍氮的利用率约35-58%.

  2. Identification of mRNA transcript and screening of amino acids in response to interaction of salinity and nitrate in aquatic fern Azolla caroliniana.

    Science.gov (United States)

    Tammam, A A; Mostafa, E M

    2012-06-01

    The mechanisms by which Azolla caroliniana respond to salt stress in absence and presence of nitrate is investigated. Screening of amino acid and differential display is used to compare overall differences in gene expression between salinity-stressed and unstressed Azolla caroliniana by quantitative reverse transcriptase polymerase chain reaction (RT-PC R). Results showed that under saline conditions, aspartic acid, glutamic acid, alanine and leucine were the amino acids found to be abundant in Azolla caroliniana, accounting for 11.26%, 8.66%, 9.43%, and 12.36%, respectively. Following salinity stress, a decrease in free glutamate concomitant with a parallel decrease in free proline was indeed evident. Interaction between nitrate and salinity stress increased proline content significantly. By screening a cDNA library, we have identified protein products by homology with known proteins. The RNA transcripts encoding protein influencing secondary metabolites and vacuolar Na+/H+ antiporter that facilitate the transport system. The databasematched under interaction of nitrate and 50 mM NaCl were associated with wall biosynthesis, disease resistance, metabolite transport and protein regulator, other gene for metabolism of steroids and secondary transport. Results obtained from this research could represent a key step in understanding the molecular mechanism of salt tolerance of Azolla caroliniana in the presence and absence of nitrate. PMID:22695523

  3. Cellular responses in the cyanobacterial symbiont during its vertical transfer between plant generations in the Azolla microphylla symbiosis.

    Science.gov (United States)

    Zheng, Weiwen; Bergman, Birgitta; Chen, Bin; Zheng, Siping; Guan, Xiong; Xiang, Guan; Rasmussen, Ulla

    2009-01-01

    The nitrogen-fixing symbiosis between cyanobacteria and the water fern Azolla microphylla is, in contrast to other cyanobacteria-plant symbioses, the only one of a perpetual nature. The cyanobacterium is vertically transmitted between the plant generations, via vegetative fragmentation of the host or sexually within megasporocarps. In the latter process, subsets of the cyanobacterial population living endophytically in the Azolla leaves function as inocula for the new plant generations. Using electron microscopy and immunogold-labeling, the fate of the cyanobacterium during colonization and development of the megasporocarp was revealed. On entering the indusium chamber of the megasporocarps as small-celled motile cyanobacterial filaments (hormogonia), these differentiated into large thick-walled akinetes (spores) in a synchronized manner. This process was accompanied by cytoplasmic reorganizations and the release of numerous membrane vesicles, most of which contained DNA, and the formation of a highly structured biofilm. Taken together the data revealed complex adaptations in the cyanobacterium during its transition between plant generations. PMID:19076717

  4. Effect of Azolla Based - Organic Fertilizer, Rock Phosphate and Rice Hull Ash on Rice Yield and Chemical Properties of Alfisols

    Directory of Open Access Journals (Sweden)

    Sudadi

    2014-07-01

    Full Text Available The application of chemical fertilizer for long time may adverse soil environment. Organic agriculture, for example combination use of azolla based-organic fertilizer, phosphate rock and rice hull ash, was one of ways that able to recover it. Research was conducted in Sukosari, Jumantono, Karanganyar while soi chemical properties analysis was analysed in Soil Chemistry and Fertility Laboratory, Fac. of Agriculture, Sebelas Maret University April to November 2013. Research design used was RAKL with 5 treatments, each repeated 5 times. The treatments applied were P0 (control, P1 ( azola inoculum dosage 250 g/m2 + phosphate rock + rice hull ash equal to 150 kg/ha KCl, P2 (azola inoculum dosage 500 g/m2 + phosphate rock equal to 150kg/ha, SP-36 + rice hull ash equal to 100 kg/ha KCl, P3 (manure dosage of 5 ton/ha,P4 (Urea 250 kg/ha + SP-36 150 kg/ha + KCl 100 kg/ha. Data analysed statistically by F test (Fisher test with level of confident 95% followed by DMRT (Duncan Multiple Range Test if any significant differences. The result showed that the treatment combination of azolla, phosphate rock and rice hull ash increase soil organic matter content, cation exchange capacity, available-P and exchangeable-K as well as rice yield ( (at harvest-dry grain weight and milled-dry grain weight.

  5. Use of Azolla to assess toxicity and accumulation of metals from artificial and natural sediments containing cadmium, copper, and zinc

    Energy Technology Data Exchange (ETDEWEB)

    Powell, G.M. [S.M. Stoller Corp., Boulder, CO (United States); Nimmo, D.W.R.; Flickinger, S.A.; Brinkman, S.F.

    1998-12-31

    The aquatic macrophyte Azolla mexicana was studied to determine if it could indicate toxicity and bioavailability of cadmium, copper, and zinc in sediments. Plants were exposed to metal-fortified artificial sediment and to natural sediment contaminated with tailings from a Superfund site near Deer Lodge, Montana. Dry weights (mass) of biomass were used to determine effects of the metal concentrations and tissue metals were measured to determine metal uptake from the sediments. Plants exposed to artificial sediments fortified with cadmium and copper showed the greatest reduction in dry mass while zinc showed the least. And, plants exposed to copper singly in artificial sediments lost both zinc and cadmium for their tissues. Plants exposed to metal-contaminated natural sediment developed necrotic and chlorotic tissue within 24 hours in 75% and 100% dilutions but significant effects (P < 0.0001) using dry mass were found as low as 3.13%.

  6. A green chemistry approach for the synthesis and characterization of bioactive gold nanoparticles using Azolla microphylla methanol extract

    Science.gov (United States)

    Kunjiappan, Selvaraj; Chowdhury, Ranjana; Bhattacharjee, Chiranjib

    2014-06-01

    This article reports the environmentally benign synthesis of gold nanoparticles (GNPs) using methanol extract of Azolla microphylla as the stabilizing and reducing agent. The GNPs were characterized by UV-vis spectrophotometry and FTIR, and the morphological characteristics were analyzed by XRD, FESEM-EDX and HRTEM. The GNPs could be formed in very short time, even in less than 30 min. The nanoparticles measured by UV-spectrophotometer demonstrated a peak at 540 nm corresponding to surface plasmon resonance spectra, and the peaks showed by FTIR suggested the presence of organic biomolecules on the surface of the GNPs. XRD results confirmed the crystalline nature of the GNPs, and FESEM-EDX and HRTEM analyses had been performed in the size ranges of 17-40 nm and 1.25-17.5 nm respectively. The synthesized GNPs showed excellent antioxidant activity. This study shows the feasibility of using plant sources for the biosynthesis of GNPs.

  7. Bimodal effect of hydrogen peroxide and oxidative events in nitrite-induced rapid root abscission by the water fern Azolla pinnata.

    Science.gov (United States)

    Cohen, Michael F; Gurung, Sushma; Birarda, Giovanni; Holman, Hoi-Ying N; Yamasaki, Hideo

    2015-01-01

    In the genus Azolla rapid abscission of roots from floating fronds occurs within minutes in response to a variety of stresses, including exposure to nitrite. We found that hydrogen peroxide, though itself not an inducer of root abscission, modulates nitrite-induced root abscission by Azolla pinnata in a dose-dependent manner, with 2 mM H2O2 significantly diminishing the responsiveness to 2 mM NaNO2, and 10 mM H2O2 slightly enhancing it. Hypoxia, which has been found in other plants to result in autogenic production of H2O2, dramatically stimulated root abscission of A. pinnata in response to nitrite, especially for plants previously cultivated in medium containing 5 mM KNO3 compared to plants cultivated under N2-fixing conditions without combined nitrogen. Plants, including Azolla, produce the small signaling molecule nitric oxide (NO) from nitrite using nitrate reductase. We found Azolla plants to display dose-dependent root abscission in response to the NO donor spermine NONOate. Treatment of plants with the thiol-modifying agents S-methyl methanethiosulfonate or glutathione inhibited the nitrite-induced root abscission response. Synchrotron radiation-based Fourier transform infrared spectromicroscopy revealed higher levels of carbonylation in the abscission zone of dropped roots, indicative of reaction products of polysaccharides with potent free radical oxidants. We hypothesize that metabolic products of nitrite and NO react with H2O2 in the apoplast leading to free-radical-mediated cleavage of structural polysaccharides and consequent rapid root abscission. PMID:26217368

  8. APLIKASI KOMBINASI KOMPOS JERAMI, KOMPOS AZOLLA DAN PUPUK HAYATI UNTUK MENINGKATKAN JUMLAH POPULASI BAKTERI PENAMBAT NITROGEN DAN PRODUKTIVITAS TANAMAN PADI BERRBASIS IPAT-BO

    OpenAIRE

    Ferina Rosiana; Tien Turmuktini; Yuyun Yuwariah; Mahfud Arifin; Tualar Simarmata

    2013-01-01

    Penelitian untuk mengetahui efek pemberian kombinasi kompos jerami dengan Azolla dan pupuk hayati majemuk terhadap peningkatan populasi bakteri penambat N dan produktivitas tanaman padi dengan teknologi IPAT-BO dilaksanakan dari bulan April hingga Juli 2012 di kebun percobaan Fakultas Pertanian, Universitas Padjadjaran, Jatinangor, dengan ketinggian + 740 m dpl. Penelitian ini menggunakan rancangan acak kelompok faktor tunggal dengan dua belas perlakuan dan tiga kali ulangan. Perlakuan terdir...

  9. Bimodal effect of hydrogen peroxide and oxidative events in nitrite-induced rapid root abscission by the water fern Azolla pinnata

    Directory of Open Access Journals (Sweden)

    Michael F Cohen

    2015-07-01

    Full Text Available In the genus Azolla rapid abscission of roots from floating fronds occurs within minutes in response to a variety of stresses, including exposure to nitrite. We found that hydrogen peroxide, though itself not an inducer of root abscission, modulates nitrite-induced root abscission by Azolla pinnata in a dose-dependent manner, with 2 mM H2O2 significantly diminishing the responsiveness to 2 mM NaNO2, and 10 mM H2O2 slightly enhancing it. Hypoxia, which has been found in other plants to result in autogenic production of H2O2, dramatically stimulated root abscission of A. pinnata in response to nitrite, especially for plants previously cultivated in medium containing 5 mM KNO3 compared to plants cultivated under N2-fixing conditions without combined nitrogen. Plants, including Azolla, produce the small signaling molecule nitric oxide (NO from nitrite using nitrate reductase. We found Azolla plants to display dose-dependent root abscission in response to the NO donor spermine NONOate. Treatment of plants with the thiol-modifying agents S-methyl methanethiosulfonate or glutathione inhibited the nitrite-induced root abscission response. Synchrotron radiation-based Fourier transform infrared (SR-FTIR spectromicroscopy revealed higher levels of carbonylation in the abscission zone of dropped roots, indicative of reaction products of polysaccharides with potent free radical oxidants. We hypothesize that metabolic products of nitrite and NO react with H2O2 in the apoplast leading to free-radical-mediated cleavage of structural polysaccharides and consequent rapid root abscission.

  10. Genetic analysis of greenbug populations of Schizaphis graminum (Hemiptera: Aphididae from Argentina and Chile based on enzyme variability Análisis genético de las poblaciones del pulgón verde Schizaphis graminum (Hemiptera: Aphididae, colectadas en Argentina y Chile, basado en su variabilidad enzimática

    Directory of Open Access Journals (Sweden)

    Luciana Saldúa

    2011-06-01

    Full Text Available Twenty-nine Schizaphis graminum (Rondani populations and sixty clones collected from very contrasting regions of Argentina and Chile were investigated electrophoretically. A high degree of enzymatic polymorphism was found. The enzymatic structure was described for the esterase system, finding nine different loci. Latitudinal stratification was determined and populations were associated into three groups, according to the latitude they were collected from. The 90% of loci resulted polymorphic in the first group and 100% of loci were polymorphic in the rest. Observed heterozygosity was lower than expected. No group had fixed alleles according to Fsr values; group one had a slight heterozygous excess, while the other groups showed slight positive Fsr values, because there was a homozygous excess. According to Frt, the third group showed the highest value that was in concordance with the highest gene flow value. Three populations could not be included in any group. The α-carboxyl-esterase was always present, also in clones and populations which were located in non agricultural zones, implicating that the insecticide resistance is well spread throughout the Argentinean and Chilean territory. No relationship was found between the enzymatic patterns and the biotype or with the host species where the aphids were collected from.Veintitrés poblaciones de Schizaphis graminum (Rondani y sesenta clones colectados en regiones muy contrastantes de la Argentina y Chile fueron investigadas electroforéticamente. Se encontró un alto grado de polimorfismo enzimático. La estructura enzimática fue descripta para el sistema estearasa y se encontraron nueve loci diferentes. Se determinó que existe estratificación latitudinal, las poblaciones fueron asociadas en tres grupos de acuerdo a la latitud donde fueron colectadas. El 90% de los loci resultaron polimórficos en el primer grupo y el 100% de los loci lo fueron en el resto. La heterocigosidad observada fue

  11. EFECTO DE LA INCORPORACIÓN DEL ABONO VERDE Azolla sp. EN LA REDUCCIÓN DE LOS DAÑOS CAUSADOS POR FITONEMATODOS EN CULTIVOS DE ORGANOPÓNICOS

    Directory of Open Access Journals (Sweden)

    R. Castro

    2009-01-01

    productores de agallas pertenecientes al género Meloidogyne sp., en los cuales se incorporó Azolla sp. como abono verde a razón de 2 kg.m-2. Los resultados mostraron que la incorporación del helecho Azolla sp. redujo significativamente los daños por nematodos fitoparásitos e incrementó a su vez los rendimientos de cultivos, tales como pepino, remolacha, zanahoria, tomate, acelga y lechuga. En este último cultivo, se comprobó que las agallas en las raíces se redujeron significativamente durante dos cosechas consecutivas.

  12. Separation of toxic rhodamine B from aqueous solution using an efficient low-cost material, Azolla pinnata, by adsorption method.

    Science.gov (United States)

    Kooh, Muhammad Raziq Rahimi; Lim, Linda B L; Lim, Lee Hoon; Dahri, Muhammad Khairud

    2016-02-01

    This study investigated the potential of untreated Azolla pinnata (AP) to remove toxic rhodamine B (RB) dye. The effects of adsorbent dosage, pH, ionic strength, contact time, and concentration were studied. Experiments involving the effects of pH and ionic strength indicated that hydrophobic-hydrophobic interactions might be the dominant force of attraction for the RB-AP adsorption system. The kinetics modelling of the kinetics experiment showed that pseudo-second-order best represented the adsorption process. The Weber-Morris intraparticle diffusion model showed that intraparticle diffusion is not the rate-limiting step, while the Boyd model suggested that film diffusion might be rate-limiting. The adsorption isotherm model, Langmuir, best represented the adsorption process, and the maximum adsorption capacity was predicted to be 72.2 and 199.7 mg g(-1) at 25 and 65 °C, respectively. Thermodynamics study indicates spontaneity, endothermic and physisorption-dominant adsorption process. The adsorbents were regenerated to satisfactory level with distilled water, HNO3 and NaOH. Pre-treatment of adsorbent with oxalic acid, citric acid, NaOH, HCl and phosphoric acid was investigated but the adsorption capacity was less than the untreated AP. PMID:26797814

  13. Separation of toxic rhodamine B from aqueous solution using an efficient low-cost material, Azolla pinnata, by adsorption method.

    Science.gov (United States)

    Kooh, Muhammad Raziq Rahimi; Lim, Linda B L; Lim, Lee Hoon; Dahri, Muhammad Khairud

    2016-02-01

    This study investigated the potential of untreated Azolla pinnata (AP) to remove toxic rhodamine B (RB) dye. The effects of adsorbent dosage, pH, ionic strength, contact time, and concentration were studied. Experiments involving the effects of pH and ionic strength indicated that hydrophobic-hydrophobic interactions might be the dominant force of attraction for the RB-AP adsorption system. The kinetics modelling of the kinetics experiment showed that pseudo-second-order best represented the adsorption process. The Weber-Morris intraparticle diffusion model showed that intraparticle diffusion is not the rate-limiting step, while the Boyd model suggested that film diffusion might be rate-limiting. The adsorption isotherm model, Langmuir, best represented the adsorption process, and the maximum adsorption capacity was predicted to be 72.2 and 199.7 mg g(-1) at 25 and 65 °C, respectively. Thermodynamics study indicates spontaneity, endothermic and physisorption-dominant adsorption process. The adsorbents were regenerated to satisfactory level with distilled water, HNO3 and NaOH. Pre-treatment of adsorbent with oxalic acid, citric acid, NaOH, HCl and phosphoric acid was investigated but the adsorption capacity was less than the untreated AP.

  14. Effect of azolla on CH4 and N2O emissions in Fuzhou Plain paddy fields%浮萍对福州平原稻田CH4和N2O排放的影响分析

    Institute of Scientific and Technical Information of China (English)

    马永跃; 仝川; 王维奇; 曾从盛

    2012-01-01

    浮萍是稻田中常见的漂浮在水面的水生植物,具有固氮作用,但是,浮萍对稻田温室气体排放的影响尚不明确.以位于湿润亚热带的福州平原稻田为研究对象,探讨浮萍对该区域稻田CH4和N2O排放的影响,为科学评价、准确编制我国水稻田温室气体排放清单提供基础数据.研究结果表明,观测期内,有萍小区和无萍小区CH4排放范围分别为0.19~26.50 mg·m-2·h-1和1.02~28.02 mg·m-2·h-1,平均值分别为9.28 mg·m-2·h-1和11.66 mg·m-2·h-1,有萍小区CH4排放低于无萍小区(P<0.01),有萍小区CH4排放高峰比无萍小区约提前1周,高峰期后排放迅速降低;有萍小区和无萍小区N2O排放范围分别为-50.11~201.82 μg·m-2·h-1和-28.93~54.42μg·m-2·h-1,平均值分别为40.29 μg·m-2·h-1和11.93 μg·m-2·h-1,有萍小区N2O排放高于无萍小区(P<0.05).稻田排干后,N2O排放迅速上升,2个小区N2O排放呈现出相似的规律.有萍小区和无萍小区的CH4与N2O排放的影响因子有所不同.综合考虑CH4和N2O两种温室气体,CH4仍是稻田温室效应产生的主要贡献者,浮萍可降低位于沿海区域的福州平原稻田综合温室效应的17.3%.%Azolla is a common aquatic fern that floats in paddy fields. It grows rapidly and has the ability to fix N2 into the soil. However, the effects of azolla growth on emissions of greenhouse gases are yet uncertain. This study measured CH4and N2O emissions in two paddy fields in Fuzhou Plain, one with floating azolla and the other with non-floating azolla. The purpose of the study was to draw on the benefits of scientific evaluation of emissions of greenhouse gases in China's paddy fields. The results showed that CR, emissions were in the range of 0.19-26.50 mg·m-2·h-1 and 1.02-28.02 mg·m-2·h-1 and with average values of 9.28 mg·m-2·h-1 and 11.66 mg·m-2h-1 in the azolla treatment and non-azolla treatment, respectively. CH4 emission in azolla plots was

  15. Afloramiento de Azolla sp. presente en el humedal Carabalo (Valle del Cauca) Colombia: metabolitos secundarios y evaluación preliminar de toxicidad

    OpenAIRE

    Carabalí Isajar, Mary Lilián

    2014-01-01

    Introducción: El espejo de agua del humedal Carabalo, ubicado en el Valle del Cauca, se encuentra completamente cubierto por una planta flotante perteneciente al género Azolla, lo cual ha afectado sus procesos biológicos y en consecuencia, se ha notado una disminución en la abundancia de peces, base de alimentación y economía en la comunidad de Quinamayó (Valle del Cauca). La sobrepoblación de esta planta ha llevado a la comunidad quinamayoreña a utilizarla como alimento para la cría de ani...

  16. Effect of Simulated Microgravity Environment on Canopy Photosynthesis Characteristics in Azolla%模拟微重力环境对红萍群体光合作用的影响研究

    Institute of Scientific and Technical Information of China (English)

    陈敏; 邓素芳; 杨有泉; 林营志; 雷锦桂

    2013-01-01

    红萍作为空间受控生态生命保障系统中的重要生物部件,为航天员提供O2和新鲜蔬菜,并吸收环境中CO2.本试验旨在弄清模拟微重力环境下红萍群体光合作用规律,为红萍生物部件的空间应用奠定基础.建立能模拟空间微重力效应的三维旋转式植物栽培装置,将红萍湿养在装置的受控密闭舱内,通过测定舱内O2和CO2浓度的变化来研究红萍群体光合作用的特征.试验结果显示,在模拟微重力环境下红萍净光合效率与光照强度成正相关,光照强度在7 000 lx时,单位能耗红萍放O2量和固定CO2量最大.红萍净光合效率还与密闭舱内大气CO2浓度环境成正相关,并与大气O2浓度环境成负相关.尤其在低O2浓度环境伴随高CO2浓度环境下,红萍的净光合效率较正常O2和CO2浓度环境的要高,这说明红萍光合放O2能力很强,有效促使密闭舱内O2和CO2浓度朝着有利于人生存的环境方向平衡,进而验证红萍的空间应用前景.%Azolla is an important biological component of the Controlled Ecological Life Support System (CELSS),because it can supply O2 and absorb CO2 from the environment,and can be used as fresh vegetable by the astronauts.The aim of this study is to clarify the canopy photosynthesis regular pattern of Azolla in simulated microgravity environment,and to lay the foundation of Azolla space's application.Azolla was moistly cultured in the controlled airtight cabin of 3D-rotary plant clinostat,which could stimulate space microgravity effect.The canopy photosynthesis characteristics of Azolla were studied by measuring the O2-CO2 concentrations in airtight cabin.The results showed that net photosynthesis efficiency of Azolla in stimulated microgravity environment had a positive correlation with illumination intensity.When illumination intensity was 7 000 lx,the amount of released O2and fixed CO2 by photosynthesis perunit energy consumption was the largest.Azolla net

  17. Potential of mosquito fern (Azolla caroliniana Willd.) plants as a biofilter for cadmium removal from waste water

    Energy Technology Data Exchange (ETDEWEB)

    Sajwam, K.S.; Ornes, W.H. [Savannah State College, GA (United States)]|[Univ. of South Carolina, Aiken, SC (United States)

    1995-12-31

    The aquatic vascular Mosquito Fern (Azolla Caroliania Willd.) was investigated as a potential biological filter for removal of Cd from waste water. Mosquito Fern plants were grown in and harvested weekly from 0.10 M Hoagland nutrient solutions containing 0.01, 0.04, and 1.03 {mu}g Cd mL{sup -1} or 0.50 M Hoagland nutrient solutions containing 0.02, 1.0, and 9.14,{mu}g Cd mL{sup -1}. Dry weights of plants significantly increased when exposed to all three Cd concentrations in 0. 10 M Hoagland solution through week three then decreased thereafter. However, in plants exposed to Cd treatments in 0.50 M Hoagland solution, dry weights increased through week one and decreased thereafter. Tissue Cd concentrations in plants grown in 0.10 M Hoagland solution increased during the first two weeks followed by decreases in week 3 and 4. However, tissue Cd increased through week 3 in plants grown in 0.50 M Hoagland solutions. Cadmium exposure to plants grown in 0.10 M Hoagland solution seemed to increase the tissue P concentrations in plants exposed to the lowest concentration of Cd. Tissue P in both control and treated plants in 0.50 M Hoagland solution seemed to increase over time with exception of the medium level (1 {mu}g Cd mL{sup -1}). These results suggest that Mosquito Fern would be useful for absorbing Cd from nutrient-rich water when the solution concentration was in the range of as low as 0.01 and as high as 9.14 {mu}g Cd mL{sup -1}. However, the harvest regime would have to be every one or two weeks to sustain plant vigor and realize maximum uptake of Cd from solution.

  18. Comparative assessment of Azolla pinnata and Vallisneria spiralis in Hg removal from G.B. Pant Sagar of Singrauli Industrial region, India.

    Science.gov (United States)

    Rai, Prabhat Kumar; Tripathi, B D

    2009-01-01

    The aim of the present work was to monitor the Hg pollution in water and sediments of G.B. Pant Sagar located in Singrauli Industrial Region, India and to suggest the efficient aquatic plants for its phytoremediation. The study assessed the comparative potential of a free floating water fern Azolla pinnata and submerged aquatic macrophyte Vallisneria spiralis to purify waters polluted by Hg. Six days laboratory experiments have been conducted to mark the percentage removal of Hg at initial concentration of 0.1, 0.5, 1.0 and 3.0 mg L(-1). The percentage removal of Hg was higher for A. pinnata (80-94%) than V. spiralis (70-84%). Likewise, the Hg accumulated in dry mass was much higher for A. pinnata and a high correlation (R(2) = 0.91 for A. pinnata and 0.99 for V. spiralis) was obtained between applied Hg doses and accumulated amounts in biomass. A concentration dependent decrease in chlorophyll a, protein, RNA, DNA and nutrients (NO(3-) and PO(4)(3-)) uptake was detected in A. pinnata and V. spiralis due to Hg toxicity. The decrease was more prominent in Azolla than Vallisneria. The results recommended the use of A. pinnata and V. spiralis to ameliorate the industrial effluents (thermal power, chlor-alkali and coal mine effluent) contaminated with Hg. PMID:18210204

  19. 7种红萍的生物学特性研究与ISSR分子标记%Biological Characteristics Seven Crossbred Azolla and ISSR Markers

    Institute of Scientific and Technical Information of China (English)

    钟珍梅; 黄勤楼; 黄毅斌; 黄秀声; 陈钟佃; 冯德庆; 唐龙飞

    2011-01-01

    Biological characteristics observation and ISSR markers were used to verify homology and distinction between crossbred Azolla, MMC3-3, MMC5-1,and MMC1-7, with the parents. The results indicated the three Azolla hybrids were separated with the parents on genotype. MMC3-3 is heat resistant, while MMC5-1 and MMC1-7 are cold resistant. The results of ISSR markers showed that MMC3-3, MMC5-land MMC1-7 were Azolla hybrids, and had differences with the parents. By clustering analysis, there was close genetic relationship between MMC3-3 with 3001, MMC1-7 with MMC5-1.%通过杂交育种育出3个杂交萍品系MMC3-3、MMC5-1、MMC1-7,采用生物学特性观测和ISSR标记方法对杂交红萍和亲本进行鉴定,验证3个杂交萍品系与亲本的同源性和区别,结果表明,3个杂交红萍与亲本在基因型上已经发生分离,其中MMC3-3较耐热,而MMC5-1、MMC1-7较耐寒.ISSR分子标记结果表明,MMC3-3、MMC 1-7和MMC5-1与亲本之间存在差异,且3个品系之间也存在差异,为杂交后代.经过聚类分析可知,MMC3-3与亲本3001遗传距离较近,MMC1-7与MMC5-1遗传距离较近.

  20. Physiological Effects of Lanthanum on Cyanobacterium Anabaena azollae%稀土元素镧对满江红鱼腥藻的生理影响

    Institute of Scientific and Technical Information of China (English)

    宋凌云; 胡文月; 赵继贞; 邵宏翔; 张昀

    2000-01-01

    对稀土元素镧(La)对满江红鱼腥藻(Anabaena azollae)的生理影响和满江红鱼腥藻对镧的富集作用进行了研究.结果显示低浓度镧对满江红鱼腥藻生长表现出促进作用,高浓度则表现出抑制作用.镧对满江红鱼腥藻的叶绿素a的合成、光合放氧活性也有影响,同样表现为低浓度促进,高浓度抑制.满江红鱼腥藻对镧的富集作用可能与其光合作用电子传递和能量合成有关.

  1. 受控生态生保系统内红萍供氧特性研究%Study on O2-supply Characteristics of Azolla in Controlled Ecological Life Support System

    Institute of Scientific and Technical Information of China (English)

    陈敏; 邓素芳; 杨有泉; 黄毅斌; 刘中柱

    2012-01-01

    In this study, we try to investigate the O2-supply characteristics under manned condition in order to lay a foundation for carrying out Azolla biological component systematical and general ground simulation experiment and space application. A closed test cabin of Controlled Ecological Life Support System and Azolla wet-culturing devices were built to measure the changes of atmospheric O2-CO2 concentration inside the cabin under "Azolla-fish-men" coexisting condition. The results showed that, O2 consumption amount per unit weight of fish is 0.0805-0.0831L.kg-1.h-1, the CO2 emission amount is 0.0705-0.0736L.kg-1.h-1. Which of trial volunteers is 02 19.71L.h-1 and CO2 18.90 L.h-1 under 7000-8000lx. Artificial light, human and fish respiring and Azolla photosynthetic O2-releasing tend to achieve O2-CO2 homeostasis inside the cabin. Enhancing atmospheric CO2 concentrations in the cabin obviously promoted Azolla group net photosynthesis efficiency. This shows that Azolla has strong photosynthetic O2-release ability, which equilibrates the O2-CO2 concentration inside the cabin in favor of human survival, and then verifies the prospect of Azolla in space application.%研究红萍载人供O_2特征,为红萍生物部件进行系统总体地面模拟试验及空间应用奠定基础,构建了受控生态生保系统密闭试验舱和红萍栽培装置,在"红萍-鱼-人"共存情况下,测定密闭舱内O_2,CO_2浓度的变化.试验结果显示,单位重量的鱼耗O_2量为0.0805~0.0831 L·kg~(-1)·h~(-1),排放CO_2量为0.0705~0.0736 L·kg~(-1)·h~(-1);试验志愿者耗O_2量19.71 L·h~(-1),呼吸释放CO_2量18.90 L·h~(-1).人工光照保持7000~8000 lx条件下,红萍的光合作用与人和鱼的呼吸作用相辅相成,舱内O_2,CO_2浓度趋于平衡.密闭舱内CO_2浓度升高对促进红萍群体净光合效率有明显效果,红萍光合放O_2能力很强,能有效促使密闭舱内O_2,CO_2浓度朝着有利于人生存的

  2. THE GENETIC DIVERSITY OF ANABAENA AZOLLAE BASED ON RAPD ANALYSIS%满江红鱼腥藻遗传多样性的RAPD分析

    Institute of Scientific and Technical Information of China (English)

    陈坚; 郑伟文; 宋铁英; 徐国忠; 唐龙飞

    2001-01-01

    @@共生在水生蕨类植物满江红(又称“红萍”:Azolla)叶腔中的固氮兰细菌曾被认为是一个种满江红鱼腥藻(Anabaena azollae Strasb.)。但由于现存的满江红在分类学上有2个亚属,7个种[1],对不同满江红叶腔中鱼腥藻的鉴别,多年来引起人们的重视。80年代,鱼腥藻的单克隆抗体和RFLP的研究,发现了它与宿主分类上一定程度的对应关系[2,3]。90年代以后,Van Coppenolle和Eskew分别以RAPD-PCR和DAF-PCR对从满江红共生体中提取的DNA进行了分析[4,5],但后者发现了共生体中藻的DNA对整个PCR产物的干扰作用会影响满江红属本身系统分类。近年来对满江红鱼腥藻的脂肪酸组分,nif基因RFLP和STRR序列PCR产物指纹分析,建立了各自的聚类分支图[6—8],其间的结果有所差异。为此作者对征集的16种不同种属和地域来源的满江红叶腔中分离出来的鱼腥藻进行了RAPD分析,探讨了满江红鱼腥藻的遗传多样性。

  3. In vitro antioxidant and hepatoprotective potential of Azolla microphylla phytochemically synthesized gold nanoparticles on acetaminophen - induced hepatocyte damage in Cyprinus carpio L.

    Science.gov (United States)

    Kunjiappan, Selvaraj; Bhattacharjee, Chiranjib; Chowdhury, Ranjana

    2015-06-01

    The present study aims to evaluate the hepatoprotective and antioxidant effects of gold nanoparticles (GNaP) biosynthesized through the mediation of Azolla microphylla and A. microphylla extract on acetaminophen-induced hepatocyte damage in common carp fish (Cyprinus carpio L.). The gold nanoparticles (100, 150, 200 μg/ml) and A. microphylla extract powder (100, 200, 400 μg/ml) were added to the primary hepatocytes in different conditions: treatment I (before 12 mM acetaminophen), treatment II (after 12 mM acetaminophen), and treatment III (both before and after 12 mM acetaminophen), and incubated. Among these, control group treated with 12 mM acetaminophen produced significantly elevated levels (50-80%) of lactate dehydrogenase (LDH), catalase (CAT), glutamate oxalate transaminase (GOT), glutamate pyruvate transaminase (GPT), and malondialdehyde (MDA), and significantly decreased the levels (60-75%) of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Treatment with methanol extract of A. microphylla phytochemically biosynthesized gold nanoparticles (100, 150, 200 μg/ml) and A. microphylla methanol extract powder (100, 200, 400 μg/ml) significantly improved the viability of cells in a culture medium. It also significantly reduced the levels of LDH, CAT, GOT, GPT, and MDA, and significantly increased the levels of SOD and GSH-Px. In conclusion, gold nanoparticles biosynthesized through A. microphylla demonstrated effective hepatoprotective and antioxidant effects than methanol extract of A. microphylla. PMID:25862331

  4. Controlled free radical attack in the apoplast: a hypothesis for roles of O, N and S species in regulatory and polysaccharide cleavage events during rapid abscission by Azolla.

    Science.gov (United States)

    Cohen, Michael F; Gurung, Sushma; Fukuto, Jon M; Yamasaki, Hideo

    2014-03-01

    Shedding of organs by abscission is a key terminal step in plant development and stress responses. Cell wall (CW) loosening at the abscission zone can occur through a combination chain breakage of apoplastic polysaccharides and tension release of cellulose microfibrils. Two distinctly regulated abscission cleavage events are amenable to study in small water ferns of the genus Azolla; one is a rapid abscission induced by environmental stimuli such as heat or chemicals, and the other is an ethylene-induced process occurring more slowly through the action of hydrolytic enzymes. Although free radicals are suggested to be involved in the induction of rapid root abscission, its mechanism is not fully understood. The apoplast contains peroxidases, metal-binding proteins and phenolic compounds that potentially generate free radicals from H2O2 to cleave polysaccharides in the CW and middle lamella. Effects of various thiol-reactive agents implicate the action of apoplastic peroxidases having accessible cysteine thiols in rapid abscission. The Ca(2+) dependency of rapid abscission may reflect the stabilization Ca(2+) confers to peroxidase structure and binding to pectin. To spur further investigation, we present a hypothetical model for small signaling molecules H2O2 and NO and their derivatives in regulating, via modification of putative protein thiols, free radical attack of apoplastic polysaccharides. PMID:24467903

  5. Study on purification of aquaculture water with Azolla%红萍净化水产养殖水体的研究

    Institute of Scientific and Technical Information of China (English)

    邓素芳; 陈敏; 杨有泉

    2009-01-01

    利用红萍(Azolla)对富营养化水产养殖水体进行净化研究.结果表明,红萍能显著增加水体中的DO,增氧幅度随流量在16.88%~70.46%之间变化,随着养殖水体流经的层数增多或者处理的时间延长,红萍对水体的增氧量加大,水中DO最终趋向一个常数值K;同时,红萍对水体中的NH3-N和TP都有明显的去除效果,NH3-N去除率随流量在9.86%~38.90%间波动,TP的去除率则随流量的变化在5.80%~38.43%之间波动.可见,红萍是净化水质的良好材料,能有效改善水产养殖水环境.利用红萍净化水体将为解决高密度集约化水产养殖的瓶颈提供新途径,实现水产养殖用水的封闭循环利用.

  6. EFECTO DE LA INCORPORACIÓN DEL ABONO VERDE Azolla sp. EN LA REDUCCIÓN DE LOS DAÑOS CAUSADOS POR FITONEMATODOS EN CULTIVOS DE ORGANOPÓNICOS

    OpenAIRE

    Castro, R; Maribel Rodríguez; Gloria E. Álvarez; M. Gil; R. Novo; R. I. Castro

    2009-01-01

    En los organopónicos, la calidad de los sustratos empleados constituye un aspecto de vital importancia, ya que con el tiempo estos pierden la riqueza en nutrientes y son propensos a la infestación con nematodos parasíticos, lo que ocasiona una disminución considerable en los rendimientos y la calidad de los cultivos. Esta investigación se realizó con el objetivo de determinar el efecto de la incorporación de Azolla sp. en las poblaciones de nematodos productores de agallas y los daños que ...

  7. Fern of the future?

    OpenAIRE

    Brinkhuis, H.; P. K. Bijl

    2014-01-01

    Held in the palm of a human hand, a single specimen of Azolla filiculoides looks downright inconsequential. Even with the scaly leaves of this miniature aquatic fern spread flat, it barely spans the distance between the creases that cleave the flesh. Its fibrous root tendrils dangle like a lock of matted hair, adding to its overall impression of impotence and making it even harder to believe that Azolla might help address humanity’s greatest challenges. That it could is the hope of a team of ...

  8. The simulation model of growth and cell divisions for the root apex with an apical cell in application to Azolla pinnata.

    Science.gov (United States)

    Piekarska-Stachowiak, Anna; Nakielski, Jerzy

    2013-12-01

    In contrast to seed plants, the roots of most ferns have a single apical cell which is the ultimate source of all cells in the root. The apical cell has a tetrahedral shape and divides asymmetrically. The root cap derives from the distal division face, while merophytes derived from three proximal division faces contribute to the root proper. The merophytes are produced sequentially forming three sectors along a helix around the root axis. During development, they divide and differentiate in a predictable pattern. Such growth causes cell pattern of the root apex to be remarkably regular and self-perpetuating. The nature of this regularity remains unknown. This paper shows the 2D simulation model for growth of the root apex with the apical cell in application to Azolla pinnata. The field of growth rates of the organ, prescribed by the model, is of a tensor type (symplastic growth) and cells divide taking principal growth directions into account. The simulations show how the cell pattern in a longitudinal section of the apex develops in time. The virtual root apex grows realistically and its cell pattern is similar to that observed in anatomical sections. The simulations indicate that the cell pattern regularity results from cell divisions which are oriented with respect to principal growth directions. Such divisions are essential for maintenance of peri-anticlinal arrangement of cell walls and coordinated growth of merophytes during the development. The highly specific division program that takes place in merophytes prior to differentiation seems to be regulated at the cellular level. PMID:23989670

  9. Competition between Free-Floating Plants Is Strongly Driven by Previously Experienced Phosphorus Concentrations in the Water Column.

    Science.gov (United States)

    Peeters, Edwin T H M; Neefjes, Rozemarijn E M; Zuidam, Bastiaan G van

    2016-01-01

    Nutrients can determine the outcome of the competition between different floating plant species. The response of floating plants to current phosphorus levels may be affected by previously experienced phosphorus concentrations because some species have the ability to store excess phosphorus for later use. This might have an impact on their competition. Here, we investigate the effect of previous and actual phosphorus concentrations on the growth rate of free-floating plant species (Azolla filiculoides, Lemna minor/gibba and Ricciocarpus natans)and the effect of phosphorus history on the competition between L. minor/gibba and A. filiculoides and between L. minor/gibba and R. natans. As expected, plant growth was lower when previously kept at low instead of high phosphorus concentrations. Growth of L. minor/gibba and A. filiculoides with a phosphorus rich history was comparable for low and high actual phosphorus concentrations, however, internal phosphorus concentrations were significantly lower with low actual phosphorus concentration. This indicates that both species perform luxury phosphorus uptake. Furthermore, internal P concentration in Azolla and Lemna increased within two weeks after a period of P deficit without a strong increase in growth. A. filiculoides in a mixture with L. minor/gibba grew faster than its monoculture. Morphological differences may explain why A. filiculoides outcompeted L. minor/gibba and these differences may be induced by phosphorus concentrations in the past. Growth of L. minor/gibba was only reduced by the presence of A. filiculoides with a high phosphorus history. Growth of L. minor/gibba and R. natans in mixtures was positively affected only when they had a high phosphorus history themselves and their competitor a low phosphorus history. These observations clearly indicate that phosphorus history of competing plants is important for understanding the outcome of the competition. Therefore, actual and previously experienced phosphorus

  10. Removal of

    Directory of Open Access Journals (Sweden)

    Roohan Rakhshaee

    2013-02-01

    Full Text Available Background and Objectives: Azolla Filiculoides as a non-living fern was used in a batch system to remove "Basic Blue 3", which is a cationic dye and a carcinogenic agent.Materials and Methods: We used a batch system by applying certain concentrations of dye contaminant and in the presence of a certain amount of adsorbent under optimum conditions. The main groups presenting in the Azolla cell wall were evaluated by acidification and alkalization of Azolla's media and then potentiometric titration with standard basic and acidic solutions. Results: It was observed that the removal efficiency of dye using non-living Azolla in accordance with the Langmuir isotherms was 82% for the initial dye concentration of 200 mg/lit under reaction conditions consisting of contact time 6 h, pH= 6, temperature 25 ˚C, and dose 5 g/lit. Qmax (maximum uptake capacity by the activated Azolla at three temperatures 5, 25 and 50 ˚C was 0.732, 0.934, and 1.176 mmol/g respectively. ΔG (Gibbs free energy changes was obtained for these temperatures as -0.457, -0.762, and -1.185 kJ/mol respectively.Conclusion: Removal of basic blue 3 using Azolla is an economically and effective method.

  11. Effets de l'alimentation des poissons avec Azolla sur la production d'un écosystème agro-piscicole en zones marécageuses au Rwanda

    Directory of Open Access Journals (Sweden)

    Kanangire, CK.

    2002-01-01

    Full Text Available Effects of Azolla-based Meal on the Production of an Agro-piscicultural Ecosystem in Rwanda Marshlands. Cette étude analyse les différences de caractères au niveau de la descendance des croisements interspécifiques entre Sesamum indicum L. et Ceratotheca sesamoides Endl. Les résultats obtenus montrent que l'héritabilité de la présence d'une forte pilosité sur les tiges et sur les pétioles est contrôlée par deux gènes assortis et indépendants. Les deux gènes dominants S et P confèrent aux plantes une importante pilosité sur les tiges et sur les pétioles tandis qu'un allèle dominant S-pp et ss-P confère aux plantes une pilosité respectivement abondante sur les tiges st sur les pétioles. Seuls les génotypes homozygotes sspp avec deux gènes récessifs produisent des plantes avec peu de poils sur les tiges et sur les pétioles. Les implications de ces résultats dans l'évolution de ces espèces sont discutées.

  12. The Nitrogen Absorption Effect of Azolla imbricata under Different Ratios of Nitrogen Source%满江红在不同氮浓度中对氮、磷的吸收效果

    Institute of Scientific and Technical Information of China (English)

    易厚燕; 吴爱平; 王华

    2013-01-01

    采用单一变量差减对比法,研究了满江红(Azolla imbricata)在氨氮、硝氮比例下的生长状况,同时检测满江红植物组织中氮含量的变化,以确定能促使满江红具最佳氮吸收效果的氨氮、硝氮浓度及比例.结果表明:不同氨氮与硝氮比例对满江红的生长存在差异,氮浓度过高和过低都不利于满江红的生长,而氨氮更有利于满江红的吸收:满江红对水中的氮、磷有很好的去除效果,在短时间内就能使水中氮、磷浓度迅速下降;植物组织中的氮、磷含量均随水中氮浓度的增加而升高,但水中较高的氮浓度可能会降低满江红的固氮作用.

  13. Diversity Analysis of Endophytic Bacteria within Azolla microphylla Using PCR-DGGE and Electron Microscopy%小叶满江红内生细菌多样性的PCR-DGGE及电子显微镜分析

    Institute of Scientific and Technical Information of China (English)

    郑斯平; 陈彬; 关雄; 郑伟文

    2008-01-01

    用16SrDNA-PCR-DGGE技术、电子显微镜和平板培养的方法系统地揭示了小叶满江红(Azolla microphylla)中内生细菌的遗传多样性和表型多样性.16S rDNA-PCR-DGGE的指纹图谱分析表明.在小叶满江红-蓝藻共生体内存在一个以蜡质芽胞杆菌(Bacillus cereus)为优势种群的复杂、多样的细菌区系;内生细菌在体内呈现各异的超微结构特征,体外培养生成不同大小、形态和色素的菌落.应用16S rDNA-PCR-DGGE技术能较全面地揭示满江红内生细菌的遗传多样性.

  14. Effects of Temperature and Phosphorus Concentration on Nitrogen and Phosphorus Uptake of Azolla imbricata%水温和磷浓度对满江红氮磷吸收的影响

    Institute of Scientific and Technical Information of China (English)

    易厚燕; 吴爱平; 庞燕; 储昭升

    2014-01-01

    通过模拟研究满江红(Azolla imbricate)在不同水温和不同磷浓度下的生长状况,了解洱海满江红生物量的变化规律及其对水中磷吸收的最佳温度和最佳磷浓度.结果表明,满江红在10 ~ 30℃均能正常生长,最适生长温度为20~25℃.满江红植物组织中的氮磷浓度及植物的相对生长率随着水中磷浓度升高而增加,其组织中的氮磷浓度在水温25℃时达到最高.表明满江红对水体中的磷有较好的吸收效果,但受到水温和水中磷浓度的交互影响,即随着水中磷浓度的增加和温度的升高,对水中磷的吸收量均增多但吸收率下降;满江红对水中磷吸收的最佳条件为水温25℃、磷浓度为0.075 mg/L.

  15. Study on the Physiological and Biochemical Responses of Azolla imbricata (Roxb.)Nakai to Ce~(3+) Stress%满江红对Ce~(3+)胁迫的生理生化应答反应研究

    Institute of Scientific and Technical Information of China (English)

    王学

    2010-01-01

    [目的] 探讨了满江红对Ce~(3+)胁迫的生理生化应答反应机制.[方法] 以水生蕨类植物满江红[Azolla imbricata (Roxb.) Nakai]为试验材料,研究了其叶绿素含量、光合放氧速率和抗氧化酶(SOD、CAT、POD)活性随Ce~(3+)浓度的变化情况.[结果]随着Ce~(3+)浓度的升高,SOD、CAT、POD活性均出现不同程度的应激性升高,而后降低;叶绿素含量、光合放氧速率呈现出先升后降的变化趋势.[结论] 在低浓度Ce~(3+)处理下,满江红通过增强其抗氧化能力,提高了其对Ce~(3+)胁迫的抵抗力;但随着Ce~(3+)浓度的加大,满江红的抗氧化能力逐渐减弱,表现出抑制效应.

  16. 镉诱导满江红(Azolla imbricata)花色素苷的初步鉴定、抗氧化活性及其作用机理%Preliminary identification, antioxidant activity and mechanism of anthocyanins from Azolla imbricata induced by cadmium

    Institute of Scientific and Technical Information of China (English)

    戴灵鹏; 董新姣; 马海虎; 盛丽梅

    2011-01-01

    In order to study the molecular structure of the anthocyanins which are induced by Cd in Azolla imbricata, anthocyanins were induced with 0.1mg·L-1 Cd, extracted with 0.1% HCI in methanol, purified by an Amberlite XAD-7 column and separated by Sephedex LH-20 column. The main compound was preliminarily identified as luteolinidin- 5-glucoside by its ultraviolet/visible absorbance spectrum and high performance liquid ( HPLC )chromatography. In vitro antioxidant properties of anthocyanins before and after purification were also evaluated by different analytical methods. The results showed that the total antioxidant capacity, reducing power, scavenging activity of superoxide anion radical (O2 - )and hydroxyl radical ( · OH )increased significantly with increasing concentrations of anthocyanin extracts, and the antioxidant activity of anthocyanins after purification was higher than the values of anthocyanins before purification. When the concentration of anthocyanins reached 0. 5 mg · mL-1, the values of anthocyanins after purification were increased by 3.16,1.36,2.71 and 1.86 times respectively, compared to anthocyanins before purification, indicating that luteolinidin-5-glucoside was the most important substance responsible for the antioxidant properties of A. imbricata. In addition, the antioxidant mechanism of anthocyanins is also discussed in this paper. In conclusion, the results obtained in this work indicated that the increase in the content of anthocyanins may be involved in internal detoxification mechanisms of A. imbricata against Cd toxicity.%为了研究镉诱导的满江红花色素苷的分子结构,分别用0.1 mg·L-1Cd、0.1%盐酸-甲醇溶液、XAD-7大孔树脂和葡聚糖凝胶SephadexLH-20柱对其进行诱导、提取、纯化和分离.经紫外可见光谱特性和HPLC图谱分析.初步确定此物质为木犀草定-5-葡萄糖苷.采用不同的体外实验方法评价了满江红花色素苷纯化前后的抗氧化活性.结果表明,花

  17. Form feature of the leaf of Azolla imbricata (Roxb.) Nakai observed lively by environmental scanning electronic microscope%满江红叶片形态特征的环境扫描电子显微镜活体观察

    Institute of Scientific and Technical Information of China (English)

    周静波; 俞斐; 沈显生

    2009-01-01

    为了克服水生植物含水量高,形貌容易变化的问题,本文利用环境扫描电子显微镜对水生蕨类植物满江红(Azolla imbricata)的形态结构进行了活体观察.满江红枝条顶端的叶片交互式覆瓦状排列,叶片圆形,几乎无气孔.位于茎中部和下部的叶片,2列互生,叶片对折抱茎,具有气孔.满江红叶片边缘具分泌细胞,幼叶的分泌细胞外具许多方形的盐结晶体,老叶的盐结晶体则脱落.结果发现,满江红具有向体外泌盐的功能.%In order to surmount higher consist of water and the changeable morphology of aquatic plants, this article describes the use of environmental scanning electronic microscope ( ESEM) to observe the morphological structure of aquatic fern Azolla imbricata alive. Azolla branches are covered by the tile-shaped leaves that are interactive, round almost no porosity in the top. The leaves located in the middle and lower stem, two alternate, leaf fold with a stoma. The secretory cells were found along the edges of new leaves to a large amount of square salt crystals. The salt crystals fall off when the leaves turned old. The results showed that A. imbricata has the function to secrete salts from the outer leaves.

  18. 介绍一种草鱼的好饲料—细绿萍

    Institute of Scientific and Technical Information of China (English)

    陶庭芸

    1982-01-01

    细绿萍(Azolla filiculoides)是从美国阿拉斯加州引进的一种肥,饲兼用的优质速生萍类。具抗寒,繁殖快,产量高、抗盐性强、怕热的特性。5℃开始生长.在-3-5℃的地区可以自然越冬。细绿萍繁殖率高.年繁殖系数平均0.123.最高达0.259.

  19. Effect of arsenic on reflectance spectra and chlorophyll fluorescence of aquatic plants.

    Science.gov (United States)

    Iriel, Analia; Dundas, Gavin; Fernández Cirelli, Alicia; Lagorio, Maria G

    2015-01-01

    Arsenic pollution of groundwater is a serious problem in many regions of Latin America that causes severe risks to human health. As a consequence, non-destructive monitoring methodologies, sensitive to arsenic presence in the environment and able to perform a rapid screening of large polluted areas, are highly sought-after. Both chlorophyll - a fluorescence and reflectance of aquatic plants may be potential indicators to sense toxicity in water media. In this work, the effects of arsenic on the optical and photophysical properties of leaves of different aquatic plants (Vallisneria gigantea, Azolla filiculoides and Lemna minor) were evaluated. Reflectance spectra were recorded for the plant leaves from 300 to 2400 nm. The spectral distribution of the fluorescence was also studied and corrected for light re-absorption processes. Photosynthetic parameters (Fv/Fm and ΦPSII) were additionally calculated from the variable chlorophyll fluorescence recorded with a pulse amplitude modulated fluorometer. Fluorescence and reflectance properties for V. gigantea and A. filiculoides were sensitive to arsenic presence in contrast to the behaviour of L. minor. Observed changes in fluorescence spectra could be interpreted in terms of preferential damage in photosystem II. The quantum efficiency of photosystem II for the first two species was also affected, decreasing upon arsenic treatment. As a result of this research, V. gigantea and A. filiculoides were proposed as bioindicators of arsenic occurrence in aquatic media. PMID:25150973

  20. Physiology and Ultrastructure of Azolla imbricata as Affected by Hg2+ and Cd2+ Toxicity%Hg2+和Cd2+胁迫对满江红生理和细胞超微结构的影响

    Institute of Scientific and Technical Information of China (English)

    施国新; 徐勤松; 解凯彬; 徐楠; 张小兰; 曾晓敏; 周红卫; 朱蕾

    2003-01-01

    The toxic effects of different gradient concentrations of Hg2+ and Cd2+ on chlorophyll content, chlorophyll a/b value, photosynthetic O2 evolution, respiration rate, anti-oxidase system (superoxide dismutase (SOD), catalase (CAT), peroxidase (POD)) and ultrastructure of the cells of Azolla imbricata (Roxb.) Nakai were studied. The results showed that with Hg2+ and Cd2+ increase, chlorophyll content and chlorophyll a/b value, photosynthetic O2 evolution decreased drastically; respiration rate peaked at 2 mg/L pollutant and declined afterwards. The activities of SOD, CAT and POD increased first and decreased afterwards except the activity of POD, which decreased with the increasing of Cd2+ concentration. Ultrastructural observation showed that the extent of ultrastructural damage was much more serious with higher pollutant concentration and longer time of stress. This resulted in swelling of chloroplast, disruption and disappearance of chloroplast membrane and disintegration of chloroplasts; swelling of cristae of mitochondria, deformation and vacuolization of mitochondria; condensation of chromatin in nucleus, dispersion of nucleolus and disruption of nuclear membrane. The experimental results showed: (1) Hg2+ and Cd2+ pollution not only destroyed physiological activities, but also caused irreversible damage to its ultrastructure, thus leading the cells to death; (2) With increase in the stress of Hg2+ and Cd2+, ultrastructural damage was related to the changes of plant physiology; (3) The toxic symptoms of plant showed an evident correlation between dose and effect; (4) The toxicity of Cd2+ on A. imbricata is heavier than that of Hg2+ under the same treatment time and concentration. The lethal concentration of Hg2+ to A. imbricata ranged from 3.5 to 4 mg/L, and that of Cd2+ ranged from 3 to 3.5 mg/L. The damage of cell ultrastructure on Anabaena azollae Strasburger was observed. The results indicated that tolerance of Azolla imbricata for Hg2+ and Cd2+ was higher

  1. Phytoremediation Potential of Aquatic Macrophyte, Azolla

    OpenAIRE

    Sood, Anjuli; Uniyal, Perm L.; Prasanna, Radha; Ahluwalia, Amrik S.

    2011-01-01

    Aquatic macrophytes play an important role in the structural and functional aspects of aquatic ecosystems by altering water movement regimes, providing shelter to fish and aquatic invertebrates, serving as a food source, and altering water quality by regulating oxygen balance, nutrient cycles, and accumulating heavy metals. The ability to hyperaccumulate heavy metals makes them interesting research candidates, especially for the treatment of industrial effluents and sewage waste water. The us...

  2. Effects of Companion Fern Azolla imbricata Extracts on Rice Growth%伴生蕨类植物满江红提取物对水稻生长的影响

    Institute of Scientific and Technical Information of China (English)

    周兵

    2011-01-01

    Using the method of laboratory bioassay, the interference of the extracts from Azolla imbricata on the growth of rice was primarily studied. The results showed that the extracts with different concentrations had influences of different degree on the seed germination and seedling growth of rice. Extracts at concentrations of 250~4 000 μg/mL had no significant effects on rice seed germination; ethanol and aqueous extracts at concentration of 1 000 μg/mL could enhance the seedling root length and plant height significantly by 27.32% and 9.15%, respectively; ethanol extracts at concentrations of 250~4 000μg/mL and aqueous extracts at concentration of 4 000 μg/mL showed significant inhibition on rice seedling height, the same as aqueous extracts at concentration of 1 000 ~4 000 μg/mL on rice seedling root length; ethanol extracts at concentrations of 2 000-4 000 μg/mL and aqueous extracts at concentration of 4 000 μg/mL on seedling fresh weight per plant, the relative highest inhibition rates was 31.85%, 14.28%, 23.41%, 16.49% and 10.94% respectively. Quadratic models were better for fitting the relationship between rice seedling root length, height, fresh weight per plant and ethahol extracts concentrations (root length: y=-0.215x2+1.541z+5.133, p=0.001; seedling height: y=-0.035x2-O.O62x+5.574, /><0.001; fresh weight per plant: y =-0.685x2 +1.484x +100.0, p<0.005), between seedling root length, height, fresh weight per plant and aqueous extracts concentrations (root length: y=-0.005x2-0276x+7.012, P<0.001; seedling height: y=-0.123x2+0.760x + 4.490, P<0.001; fresh weight per plant: y=-1.742x2+10.49x+89.27, p<0.005).%通过室内生物测定的方法研究满江红提取物对水稻种子萌发及幼苗生长的影响,并就提取物浓度与水稻生长相关指标间的关系进行回归分析.结果表明,与对照相比,250~4 000 μg/mL的提取物对水稻种子萌发不产生显著影响;1 000 μg/mL的醇提物和水提物分别对水稻幼苗根长

  3. Aquatic arsenic: phytoremediation using floating macrophytes.

    Science.gov (United States)

    Rahman, M Azizur; Hasegawa, H

    2011-04-01

    Phytoremediation, a plant based green technology, has received increasing attention after the discovery of hyperaccumulating plants which are able to accumulate, translocate, and concentrate high amount of certain toxic elements in their above-ground/harvestable parts. Phytoremediation includes several processes namely, phytoextraction, phytodegradation, rhizofiltration, phytostabilization and phytovolatilization. Both terrestrial and aquatic plants have been tested to remediate contaminated soils and waters, respectively. A number of aquatic plant species have been investigated for the remediation of toxic contaminants such as As, Zn, Cd, Cu, Pb, Cr, Hg, etc. Arsenic, one of the deadly toxic elements, is widely distributed in the aquatic systems as a result of mineral dissolution from volcanic or sedimentary rocks as well as from the dilution of geothermal waters. In addition, the agricultural and industrial effluent discharges are also considered for arsenic contamination in natural waters. Some aquatic plants have been reported to accumulate high level of arsenic from contaminated water. Water hyacinth (Eichhornia crassipes), duckweeds (Lemna gibba, Lemna minor, Spirodela polyrhiza), water spinach (Ipomoea aquatica), water ferns (Azolla caroliniana, Azolla filiculoides, and Azolla pinnata), water cabbage (Pistia stratiotes), hydrilla (Hydrilla verticillata) and watercress (Lepidium sativum) have been studied to investigate their arsenic uptake ability and mechanisms, and to evaluate their potential in phytoremediation technology. It has been suggested that the aquatic macrophytes would be potential for arsenic phytoremediation, and this paper reviews up to date knowledge on arsenic phytoremediation by common aquatic macrophytes. PMID:21435676

  4. Potential of some aquatic plants for removal of arsenic from wastewater by green technology

    Directory of Open Access Journals (Sweden)

    Mohammed Barznji Dana A.

    2015-03-01

    Full Text Available Phytoremediation or green technology is counted among the successful and effective biological contaminated water treatment techniques. Basically, the concept of this green, cost-effective, simple, environmentally nondisruptive method consists in using plants and microbiological processes to reduce contaminants in the ecosystem. Different species from aquatic plants (emerged, free-floating, and submerged have been studied to mitigate toxic contaminants such as arsenic, cadmium, chromium, copper, lead, mercury, zinc, etc. Arsenic is one of the most severe toxic elements; it is widely distributed in the environment, usually found in combination with chloride, oxygen, sulphur and metal ions as a result of mineral dissolution from sedimentary or volcanic rocks and the dilution of geothermal water. The effluents from both industrial and agricultural sectors are also regarded as sources to contaminate water. From the accumulation point of view, several aquatic plants have been mentioned as good arsenic accumulators and their performance is evaluated using the green technology method. These include Spirodela polyrhiza, Wolffia globosa, Lemna gibba, L. minor, Eichhornia crassipes, Azolla caroliniana, Azolla filiculoides, Azolla pinnata, Ceratophyllum demersum and Pistia stratiotes. The up-to-date information illustrated in this review paper generates knowledge about the ability of some common aquatic plants around the globe to remediate arsenic from contaminated water.

  5. Male fertility of species and interspecific hybrid among subgenus Euazolla%满江红三膘亚属种及种间杂种雄性育性初步研究

    Institute of Scientific and Technical Information of China (English)

    金桂英; 陈坚; 唐龙飞

    2003-01-01

    报道满江红三膘亚属(Euazolla)4个种--蕨状满江红(Azolla filiculoides)、小叶满江红(A.microphylla)、墨西哥满江红(A.mexicana)和卡洲满江红(A.Caroliniana)小孢子育性特点;卡洲满江红2个品种,其它3个种及种间杂种F1代小孢子育性特点.影响小孢子育性的两个主要因素是外界环境条件和遗传因素.讨论了小孢子育性研究对满江红物种划分的意义.

  6. Invasive vascular plant species of oxbow lakes in south-western Poland

    Directory of Open Access Journals (Sweden)

    Spałek Krzysztof

    2015-06-01

    Full Text Available Natural water reservoirs are very valuable floristic sites in south-western Poland. Among them, the most important for the preservation of biodiversity of flora are oxbow lakes. The long-term process of human pressure on habitats of this type caused disturbances of their biological balance. Changes in the water regime, industrial development and chemisation of agriculture, especially in the period of the last two hundred years, led to systematic disappearances of localities of many plant species connected with rare habitats and also to the appearance of numerous invasive plant species. They are: Azolla filiculoides, Echinocystis lobata, Erechtites hieraciifolia, Impatiens glandulifera, I. parviflora, Reynoutria japonica, Solidago canadensis, S. gigantea and S. graminifolia. Field works were conducted in years 2005-2012.

  7. 纳米TiO2对卡洲萍同步富集水体中Cu(Ⅱ)与Cr(Ⅵ)的影响研究%A Study on Simultaneous Bioaccumulation of Cu (Ⅱ) and Cr (Ⅵ) by Azolla caroliniana in the Presence of TiO2 Nanoparticles

    Institute of Scientific and Technical Information of China (English)

    游海萍; 汪婷; 徐国忠; 陈祖亮

    2013-01-01

    研究了投加不同质量浓度纳米二氧化钛(nTiO2)颗粒对卡洲萍(Azolla aroliniana)同步富集混合溶液中Cu(Ⅱ)和Cr(Ⅵ)的影响.结果显示,卡洲萍同步富集Cu(Ⅱ)和Cr(Ⅵ)时,对Cu(Ⅱ)和Cr(Ⅵ)的富集量分别为12.02 mg·g-1和4.17 mg·g-1,对Cu(Ⅱ)的富集效率明显高于Cr(Ⅵ).当nTiO2的投加量为300 mg·L-1时,卡洲萍对Cu(Ⅱ)和Cr(Ⅵ)的富集量分别下降50.8%和14.3%.红外分析显示卡洲萍在nTiO2存在的条件下可以诱导产生更多的蛋白质,核酸等物质,这些物质可以增强植物的抗逆性,以减轻重金属毒害;SEM和EDS图谱表明Cu(Ⅱ)和Cr(Ⅵ)吸附在nTiO2颗粒上,增大进入植物体的难度,降低了进入萍体中重金属的量;且nTiO2质量浓度较高时本身也会对卡洲萍产生毒性,胁迫植物体分泌一些高分子生物聚合物,影响植物吸收Cu(Ⅱ)和Cr(Ⅵ).最后,通过回归分析结果显示,卡洲萍富集重金属阳离子Cu(Ⅱ)和重金属阴离子Cr(Ⅵ)的响应机制相同,与重金属离子的电性无关.本文研究结果表明nTiO2进入水体,与水体中重金属离子相互作用,将Cu(Ⅱ)和Cr(Ⅵ)吸附在纳米颗粒表面,而增大其进入植物体的难度,影响其与水生植物的相互作用.

  8. Detección de compuestos presentes en una especie de Amanita micoparasitada, colectada en el corregimiento de Santa Elena (Antioquia, Colombia

    Directory of Open Access Journals (Sweden)

    Londoño Liliana

    2005-12-01

    Full Text Available Para este trabajo se seleccionó una especie de Amanita, parasitada probablemente por un Hyphomycete, que presenta un crecimiento anormal y sobre la cual no existen trabajos previos. Inicialmente se hicieron extracciones etanólicas del material fresco en frío y con soxhlet. Se realizó una marcha fotoquímica preliminar del extracto obtenido y se detectaron coumarinas, alcaloides, fenoles y glicósidos cardiotónicos. Posteriormente se estandarizó que la cromatografía de capa
    fina con el sistema hexano:acetona (7:3 permitió apreciar el mejor perfil cromatográfico. Con este sistema se inició una cromatografía de columna eluida a gradiente de la cual se obtuvieron 21 fracciones, que se agruparon en tres fracciones finales de acuerdo a su perfil cromatográfico. Para la cromatografía de capa fina de las fracciones se usó el sistema diclorometano:etanol (9,5:0,5 que presentó una buena separación. Finalmente se detectaron ocho compuestos mayoritarios caracterizados por su factor de retención y su patrón de coloración. Como aporte al conocimiento de esta especie sería recomendable un trabajo posterior para purificar estos compuestos y realizar su elucidación estructural. Actualmente, se desarrollan trabajos taxonómicos para determinar la especie de Amanita e identificar el agente parásito.

  9. 从"Azolla Event"论满江红在生态农业中的应用%Application of Azolla in Ecological Agriculture from Azolla Event

    Institute of Scientific and Technical Information of China (English)

    黄毅斌; 翁伯琦; 唐龙飞

    2010-01-01

    从分析始新世满江红在地球气候从"温室效应"向"冰室效应"转变的过程所起的作用入手,指出满江红具有在未来生态农业应用的潜力,包括作为优质农产品的生产原料、作为控制农业生态环境和重金属污染的植物、作为外太空开发的先行植物等.并提出要加强满江红在节能、环保和有机肥源、外源基因导入和满江红孢子果生产技术的研究以充分发挥满江红在未来生态农业中的作用.

  10. Effects of cadmium on nitrogen metabolism in Azolla imbricata-Anabaena azollae symbiosis%镉对满江红(Azolla imbricata)-鱼腥藻(Anabaena azollae)共生体氮代谢的影响

    Institute of Scientific and Technical Information of China (English)

    戴灵鹏; 熊治廷; 马海虎

    2009-01-01

    在实验室条件下,研究了不同浓度(0、0.01、0.05、0.1、0.5mg/L)的Cd对满江红-鱼腥藻共生体异型胞频率,固氮酶、谷氨酰氨合成酶活性以及铵态氮、游离氨基酸、可溶性蛋白、总氮含量的影响.结果表明,在整个实验期间,0.01mg/L Cd处理对上述指标均没产生显著影响,说明满江红-鱼腥藻共生体对Cd具有较强的耐性.当培养液中Cd浓度≥0.05mg/L时,随溶液中Cd浓度的增加和处理时间的推移,异型胞频率、固氮酶活性、谷氨酰氨合成酶活性、可溶性蛋白含量和总氮含量逐渐下降,而铵态氮含量在处理初期显著降低,随后迅速增加,游离氨基酸含量则逐渐增加.研究结果表明高浓度的Cd处理导致满江红-鱼腥藻共生体氮代谢的紊乱,最终造成氮素积累量的下降.

  11. Can we predict community-wide effects of herbicides from toxicity tests on macrophyte species?

    Science.gov (United States)

    Coutris, Claire; Merlina, Georges; Silvestre, Jérôme; Pinelli, Eric; Elger, Arnaud

    2011-01-17

    Macrophyte communities play an essential role in the way freshwater ecosystems function. It is thus of great concern to understand how environmental factors, especially anthropogenic ones, influence their composition and diversity. The aim of this study was to examine whether the effects of a herbicide mixture (50% atrazine, 35% isoproturon, 15% alachlor) on single macrophyte species can be used to predict its impact at a community level. In a first experiment we tested the sensitivity of six species (Azolla filiculoides, Ceratophyllum demersum, Elodea canadensis, Lemna minor, Myriophyllum spicatum and Vallisneria spiralis) grown separately and exposed to 0.6-600 μg L(-1) of the herbicide mixture. In a second experiment, conducted in microcosms, we tested the effects of herbicides on macrophyte assemblages composed of the same six species exposed to 0, 6 or 60 μg L(-1) of the herbicide mixture. Species grown separately exhibited growth inhibition at 60 and 600 μg L(-1). At 600 μg L(-1) the sensitivity differed significantly between species. V. spiralis was the most resistant species, C. demersum, M. spicatum and E. canadensis exhibited intermediate sensitivities, and A. filiculoides and L. minor were the most sensitive species. In microcosms, community biomass and Shannon evenness index were reduced after 8 weeks at 60 μg L(-1). Communities also exhibited changes in their composition: the relative and absolute abundance of C. demersum increased at 6 μg L(-1), while the relative abundance of V. spiralis increased at 60 μg L(-1). These results are in agreement with the individual responses of these species to the herbicides. It is therefore concluded that short-term effects of herbicides on simple macrophyte communities can be predicted from the sensitivity of individual species. However, further investigations are required to examine whether longer term effects can be predicted as well, especially in more complex communities. PMID:20926143

  12. Genome erosion in a nitrogen-fixing vertically transmitted endosymbiotic multicellular cyanobacterium.

    Directory of Open Access Journals (Sweden)

    Liang Ran

    Full Text Available BACKGROUND: An ancient cyanobacterial incorporation into a eukaryotic organism led to the evolution of plastids (chloroplasts and subsequently to the origin of the plant kingdom. The underlying mechanism and the identities of the partners in this monophyletic event remain elusive. METHODOLOGY/PRINCIPAL FINDINGS: To shed light on this evolutionary process, we sequenced the genome of a cyanobacterium residing extracellularly in an endosymbiosis with a plant, the water-fern Azolla filiculoides Lam. This symbiosis was selected as it has characters which make it unique among extant cyanobacterial plant symbioses: the cyanobacterium lacks autonomous growth and is vertically transmitted between plant generations. Our results reveal features of evolutionary significance. The genome is in an eroding state, evidenced by a large proportion of pseudogenes (31.2% and a high frequency of transposable elements (approximately 600 scattered throughout the genome. Pseudogenization is found in genes such as the replication initiator dnaA and DNA repair genes, considered essential to free-living cyanobacteria. For some functional categories of genes pseudogenes are more prevalent than functional genes. Loss of function is apparent even within the 'core' gene categories of bacteria, such as genes involved in glycolysis and nutrient uptake. In contrast, serving as a critical source of nitrogen for the host, genes related to metabolic processes such as cell differentiation and nitrogen-fixation are well preserved. CONCLUSIONS/SIGNIFICANCE: This is the first finding of genome degradation in a plant symbiont and phenotypically complex cyanobacterium and one of only a few extracellular endosymbionts described showing signs of reductive genome evolution. Our findings suggest an ongoing selective streamlining of this cyanobacterial genome which has resulted in an organism devoted to nitrogen fixation and devoid of autonomous growth. The cyanobacterial symbiont of Azolla

  13. Genetic Characterization of Old Grapevines collected in Oases of the Atacama Desert Caracterización Genética de Vides Antiguas colectadas en Oasis del Desierto de Atacama

    OpenAIRE

    Ingrid Poblete; Manuel Pinto; María Teresa de Andrés; Patricio Hinrichsen

    2011-01-01

    Old grapevine (Vitis vinifera L.) accessions are a source of genes that could be rescued for use per se or in modern breeding programs. The first step in this rescuing is collecting and characterizing the germplasm from a particular region. This study presents the genetic characterization of 21 grapevine accessions collected from the Atacama Desert in the far North of Chile. Characterization was based on 12 microsatellites (Simple Sequence Repeats, or SSRs) supplemented with Amplified Fragmen...

  14. Contaminación y viabilidad de huevos de Toxocara spp. en suelo y heces colectadas en parques públicos, calles y perros en Toluca, México

    OpenAIRE

    Camilo Romero-Núñez; Selene Yañez-Arteaga; Germán David Mendoza-Martínez; Lilia Patricia Bustamante-Montes; Ninfa Ramírez-Durán

    2013-01-01

    Se realizó un estudio de contaminación y viabilidad de huevos de Toxocara spp, parásito zoonótico en la ciudad de Toluca, México, para lo cual se colectaron 2374 muestras de suelos de siete parques, 350 de suelo de jardines de casas, 199 heces de perros con propietario y 199 heces obtenidas de la vía pública en las áreas cercanas a los parques. En los parques, la conta- minación con Toxocara fue de 24,7% y la viabilidad de 73,3%, siendo menor en los jardines de casa (13,9 y 40,3%, respe...

  15. Aprovechamiento de desechos orgánicos: 1. Extracción y caracterización del aceite de semillas de naranja colectadas en expendios ambulantes de jugos

    Directory of Open Access Journals (Sweden)

    María Escalante

    2012-12-01

    Full Text Available The Soxhlet extraction, using n-hexane as solvent, of the orange seed oil from waste residues obtained in street sales of fruit juices of Merida, Venezuela, was studied. Yields of 5.1% oil (w/w and 42.7% (w/w relative to the initial mass of the residue and dry seeds, respectively, were obtained. The oil was subjected to a transesterification process with methanol and the main components of the mixture of methyl esters achieved were quantified by gas chromatography coupled to mass spectrometry (GC-MS, which turned out to be: linoleate (311%, oleate (311%, palmitate (303% and magnesium stearate (7.10.5%. The mixture of methyl esters was subjected to the hydrolysis reaction in aqueous alkaline medium yielding the respective acid mixture which, after a precipitation process in HCl aqueous solution and its re-crystallization in n-hexane, generated an acid mixture with a higher content of unsaturated acids.

  16. Action of Azolla on Model of Rice-azolla-fish and Rice-azolla-duck%红萍在"稻萍鱼"、"稻萍鸭"模式中的作用

    Institute of Scientific and Technical Information of China (English)

    杨有泉; 陈敏

    2008-01-01

    在"稻萍鱼"、"稻萍鸭"等有机水稻栽培模式中,红萍平生于稻田水面,生长繁殖快,产量高,既为水稻提供充分的肥料源,又可作为鱼鸭等动物的青饲料.放养红萍可有效地提高水稻田的生产力,并明显促进稻田生态环境的持续良性循环.

  17. A sensitive and quantitative biosensing method for the determination of {gamma}-ray emitting radionuclides in surface water

    Energy Technology Data Exchange (ETDEWEB)

    Wolterbeek, H.Th.; Meer, A.J.G.M. van der [Technische Univ. Delft (Netherlands). Interfacultair Reactor Inst.

    1996-11-01

    A quantitative and sensitive biosensing method has been developed for the determination of {gamma}-ray emitting radionuclides in surface water. The method is based on the concept that at equilibrium the specific radioactivity in the biosensor is equal to the specific radioactivity in water. The method consists of the measurement of both the radionuclide and the element in the biosensor and the determination of the element level in water. This three-way analysis eliminates problems such as unpredictable biosensor behaviour, effects of water elemental composition or further abiotic parameters: what remains is the generally high enrichment (bioaccumulation factor BCF) of elements and radionuclides in the biosensor material. Measurements were performed with floating water plants (Azolla filiculoides Lamk., Spirodela polyrhiza/Lemna sp.) and the fully submerged water plant Ceratophyllum demersum L., which were sampled from ditch water. Concentrations of elements and radionuclides were determined in both water and biosensor plants, using Neutron Activation Analysis (NAA), ICP-MS, and {gamma}-ray spectrometry, respectively. For the latter, both 1 litre samples (Marinelli-geometry) and 1 cm{sup 3} samples (well-type detectors) were applied in measurements. (author).

  18. The use of water lettuce (Pistia stratiotes L.) for rhizofiltration of a highly polluted solution by cadmium and lead.

    Science.gov (United States)

    Veselý, Tomás; Tlustos, Pavel; Száková, Jirina

    2011-10-01

    The effectiveness of heavy metal uptake from contaminated nutrient solution by four aquatic macrophytes (Pistia stratiotes L., Salvinia auriculata AubL, Salvinia minima Baker, and Azolla filiculoides Lam) was estimated in this study. The influence of cadmium (3.5 mg L(-1) and 10.5 mg L(-1)) and lead (25 mg L(-1) and 125 mg L(-1)) on the stress symptoms was observed through the determination of chlorophyll content and transpiration rate over 14 days of the experiment. The results of the present study showed extreme reductions in Cd and Pb concentrations in solution during the first 4 days. The accumulation of Pb in plant tissues was the highest during the first 4 days and was more than 10 times higher in the roots (42,862 mg kg(-1)) than in the leaves (3867 mg kg(-1)). The accumulation of Cd slowly increased and was the highest at the end of the experiment. Concentrations in roots (3923 mg kg(-1)) were roughly 6 times higher than in the leaves (624 mg kg(-1)). Results showed significant decrease in the transpiration rate at Pb treatment and a significant increase at Cd treatment during 48 hours of exposition. PMID:21972509

  19. Pleistocene pollen stratigraphy from borehole 81/34, devil's hole area, central north sea

    Science.gov (United States)

    Ekman, Sten R.

    1998-09-01

    Twelve pollen assemblage zones are identified in a 229 m deep borehole (BH 81/34) from the Devil's Hole area in the central North Sea (British sector). The sediment from this borehole is Early to Late Pleistocene in age and the observation of massulae from Azolla filiculoides in sediment with reversed polarity indicates an age younger than the Olduvai geomagnetic event for the entire sequence. The Early Pleistocene sediments were at least partly deposited in the vicinity of a river outlet and can be correlated either with the Eburonian or the Menapian cold stage and with the Bavel interglacial and the Linge glacial within the Bavelian stage in the Dutch stratigraphy. The Middle Pleistocene sequence contains an interval rich in Abies, Picea and Pinus, probably deposited during the end of either Cromerian Complex interglacial IV (Noordbergum) or possibly the Holsteinian. The uppermost 80 m of the core contains high frequencies of pre-Quaternary and deteriorated palynomorphs indicating extensive glacial or glaciofluvially reworked sediment.

  20. Early and Middle Pleistocene vegetation history of the Médoc region, southwest France

    Science.gov (United States)

    O'Brien, C. E.; Jones, R. L.

    2003-09-01

    Pleistocene deposits, together with their pollen, plant macrofossil, foraminiferal, dinoflagellate and coleopteran remains, from five sites along the Atlantic coast of the Médoc Peninsula are described and discussed. Sediments making up the Négade Formation are shown to have been laid down under either estuarine or lagoonal conditions when closed Quercus-Pinus-Tsuga canadensis regional woodland existed. Comparison with plant records from The Netherlands indicates that these deposits are most likely attributable to either the Early Pleistocene Bavel Interglacial (marine oxygen isotope stage (MIS) 31), or an interglacial of the Waalian (MIS 37-49) or Tiglian (MIS 63-79). In addition, clays assigned to the Argiles du Gurp sensu stricto, were similarly deposited in either an estuary or lagoon, which subsequently was cut off from the sea. A freshwater lake with vegetation dominated by Azolla filiculoides then developed. This was succeeded by reedswamp and an organic mud (termed Lignite in the corresponding French stratigraphical records) formed. Regional Quercus-Abies woodland was replaced by one with Pinus dominant and Pterocarya a minor component. Comparison with plant records from France and other parts of Europe suggest that the clays and organic mud might be correlated with the Holsteinian (Praclaux) Interglacial (MIS 11c). Copyright

  1. Biting midges (Diptera: Ceratopogonidae) from Martín García Island, Argentina.

    Science.gov (United States)

    Ronderos, María M; Marino, Pablo I; Díaz, Florentina; Estévez, Ana L

    2011-09-01

    Nearly 230 species of biting midges have been recorded or described from Argentina; 38 of them are known from the Buenos Aires province and only one is cited from Martín García Island. This paper presents the results raised from six collecting trips which took place on the island during spring 2005, summer 2006 and autumn 2009. Diverse sampling sites including permanent and temporary aquatic environments were chosen, most of the ten sampling sites were ponds of diverse origin, some of these environments were covered with floating vegetation as Lemna gibba, Lemna minuscule, Salvinia biloba, Salvinia minima, Azolla filiculoides, Limnobium laevigatum, Pistia stratiotes, Spirodela intermedia, Wolffiella oblonga and Wolffia columbiana. Other sites were placed in urban and suburban areas. Adults were collected with sweep nets at sunrise and sunset and with light traps at intervals of four to five hours at night, depending on electricity availability on the island. Larvae and pupae were collected with different implements depending on characteristics of each surveyed aquatic habitat. In free standing water, they were captured with small sieves or hand pipettes and micropipettes, flotation techniques were utilized for sampling vegetated areas, free and rooted floating hydrophytes were extracted for removing insects among them. Thirteen species of Ceratopogonidae were collected, three of Atrichopogon Kieffer, three of Forcipomyia Meigen, two of Dasyhelea Kieffer, four of Culicoides Latreille, and one of Bezzia Kieffer, all representing new records from the island. PMID:22017124

  2. Adsorption and Uptake of Cadmium by Azolla pinnata:Kinetics of Inhibition by Cations

    Institute of Scientific and Technical Information of China (English)

    J.P.GAUR; N.NORAHO

    1995-01-01

    A.pinnata showed a remarkable ability of taking up Cd from the external medium.Of the total Cd taken up by the test plant,surface adsorption was about 90%.Cd adsorption did not occur at a constant rate,however,an equilibrium was reached in 2h.The uptake of Cd occurred at a constant rate.Test cations.Including heavy metals(Ca,Mg,K,Na,Ni,F,Cu and Zn),inhibited adsorption as well as uptake of Cd.Cd adsorption was competitively inhibited by Cu,Fe and Zn,whereas Ca,Mg,K,Na and Ni caused non-competitive inhibition,Similarly,Cd uptake was competitively(Ca,Mg,Fe)or non-competitively(Na,K,Ni,Zn) inhibited by cations.Inhibition of Cd uptake by Cu was not wholly competitive.

  3. The Equipment of Using AZOLLA for O2-Supplimentation and its Test

    Science.gov (United States)

    Liu, Xia-Shi; Chen, Min; Bian, Zu-Liang; Liu, Chung-Chu

    The water-consuming amount in a long-term astro-navigation is large. In order to reduce the burden of water supply from Earth ground, the space station needs to resolve the problems of water supply. For this reason, the recovery and regeneration of urine solution of spacemen, and its utilization possess a key importance. Many investigations on this aspect have been reported. Our research based on "biological absorption-purification-UV photocatalytic oxidation" techniques with a relevant treating equipment that for a comprehensive treatment to fresh urine of spacemen has been created. In this equipment, the urine solution was used as the nutrient solution for the biological parts in ecological life ensurant system, after absorbing the nutrient, it was decomposed, metabolized and purified in some distance, and created a favorable condition for the follow-up oxidation treatment by UV-Photocatalytic Oxidation. After these two processes, the treated urine solution reached the GB5749-85 standard of water quality. Some main indexes are as table one. Chroma<5-15 Feculent degree-NTU-1.20-3 --5 Total rigidity-according to CaCO3-mg/L-3.60-450 N-NO3--mg/L-0.60-20 Soluble total solid-mg/L-543-1000 Bacterial gross-cfu/ml-13-100 Coliform group-No./L-<3-3 Key words-Photocatalytic Oxidation , Ultraviolet

  4. Pengaruh Komposisi Azolla dan Sampah Organik Kota terhadap Kualitas Kompos yang Dihasilkan

    OpenAIRE

    Lubis, Dian Bastian

    2012-01-01

    Sampah kota merupakan salah satu jenis bahan organik yang belum dikelola baik hingga saat ini. Pengomposan sampah kota akan bermanfaat mengatasi masalah lingkungan menjadi sumber bahan organik bagi lahan pertanian dan memberikan nilai tambah ekonomi. Penelitian ini dilakukan di laboratorium Biologi Tenah Fakultas Pertanian Universitas Sumatera Utara, Medan. Penelitian ini dilakukan pada pertengahan bulan Mei sampai akhir bulan Mei 2004. Penelitian ini merupakan percobaan non faktorial, ...

  5. Studies on urine treatment by biological purification using Azolla and UV photocatalytic oxidation

    Science.gov (United States)

    Liu, Xiaofeng; Chen, Min; Bian, Zuliang; Liu, Chung-Chu

    The amount of water consumed in space station operations is very large. In order to reduce the amount of water which must be resupplied from Earth, the space station needs to resolve the problems of water supply. For this reason, the recovery, regeneration and utilization of urine of astronauts are of key importance. Many investigations on this subject have been reported. Our research is based on biological absorption and, purification using UV photocatalytic oxidation techniques to achieve comprehensive treatment for urine. In the treatment apparatus we created, the urine solution is used as part of the nutrient solution for the biological components in our bioregenerative life support system. After being absorbed, the nutrients from the urine were then decomposed, metabolized and purified which creates a favorable condition for the follow-up oxidation treatment by UV photocatalytic oxidation. After these two processes, the treated urine solution reached Chinese national standards for drinking water quality (GB5749-1985).

  6. Desarrollo de una metodología in vitro para estimar la tasa de fermentación de los forrajes en el intestino grueso del cerdo

    Directory of Open Access Journals (Sweden)

    Leterme Pascal

    2005-12-01

    Full Text Available La metodología para estimar la tasa de fermentación de los forrajes se desarrolló en dos etapas. La primera, de digestibilidad enzimática, simula la digestión ocurrida en el estómago e intestino delgado, mediante el empleo de pepsina y pancreatina; en esta se determina la digestibilidad de la materia seca. La segunda etapa de fermentación simula la fermentación que ocurre en el intestino grueso utilizando como inóculo heces de cerdo; en esta se determina la cantidad de gas producido durante la fermentación de los forrajes y la degradación de la fibra detergente neutra (FDN. Las dos etapas presentaron buena repetibilidad en el tiempo. Abstract Develop of an in vitro methodology to determine the forages fermentation in to the large intestine. The methodology was developing in two phases. The first phase (enzymatic digestibility simulates the digestion occurred in the stomach and small intestine, by means of the employment of pepsin and pancreatin; and the digestibility of the dry matter is determined. The second phase (in vitro fermentation simulates the fermentation that occurs in the large intestine utilizing pig faeces; the quantity of gas produced during the fermentation of the feedstuff is determined and determine the degradation of neutral detergent fiber (NDF. All the phases show a good repeatability throw the time. Key words: digestibility in vitro, pigs, fermentación, forages (Azolla filiculoides, Xanthosoma saggitifolium, Morus alba, Trichanthera gigantea y Salvinia molesta.

  7. Protein (Cyanobacteria): 175822 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available agen triple helix repeat-containing protein 'Nostoc azollae' 0708 MRLIEDGEDGEDGEDGEDGEDGEDGEDGEDGEDGEDGEDGEDGGEIFLMPYALCPMPYALCPMPYALCPMPYALCPMPYALCPMPYAQNQDFSHPNRESSVKLFSSVAPKP ...

  8. Protein (Cyanobacteria): 365536 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available othetical protein Aazo_2182 'Nostoc azollae' 0708 MNIHTFDNNYVTCPICQRNNRTKPMKTYIGLFTCPYCQERLVVCNSGHYVRDPFTWEEIVIASALARQSYPLARMIRNLVLLQRPFVALAMGCAVLLTTIAVTQENISYNNPLVGENEKVIKPGKDYIP ...

  9. Protein (Cyanobacteria): 466100 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available othetical protein Aazo_2366 'Nostoc azollae' 0708 MVIELMVSSIASVVTRSVIPTMIDFIIIYTSTSPITSNMSSRMTTTIIMICISVFKNTQQRHSRYHCSENNRSNWFHEDNLKSLVMISRSVGRKFNKRRLHQALNRFNQQMFQNYSILRILFNSLTD ...

  10. Protein (Cyanobacteria): 56219 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available ndent nitrogenase family protein 'Nostoc azollae' 0708 MKVFESTTKKIFFASSVSLHEAETKKADVSQLQVSYSQSAWNFLRPFRDWLDNLKVGDRQFAHRLCQLIPAQCPFERDVKLFGKTLFHIPPMCKLNPLYEEVVALRFRALCYLADECGEDISQYC ...

  11. 高产高蛋白饲草满江红(Azolla imbricata)放养技术研究初报%Cultivation Techniques of Azolla with High Yield and Protein

    Institute of Scientific and Technical Information of China (English)

    刘一; 樊廷录; 唐小明

    2000-01-01

    满江红属蕨类植物,以营养体的侧枝断离无性繁殖方式为主,粗蛋白、赖氨酸、磷、粗脂肪含量均高于紫花苜蓿.研究结果表明,群体延续时间为7 d时,繁殖系数最高,在西北地区4~9月份的适宜生长季节,采用薄膜微水放养技术,每公顷水面生产鲜草约450 t,折合干重45 t,粗蛋白11.25 t,可作为优质饲料开发利用.

  12. 镉胁迫对满江红-鱼腥藻共生体供氮能力的影响%Effects of Cd stress on nitrogen supply ability of Azolla imbricata-Anabaena azolla symbiosis

    Institute of Scientific and Technical Information of China (English)

    詹玲君; 马海虎; 南旭阳; 张业建; 陈访; 周舟; 戴灵鹏

    2015-01-01

    在实验室条件下,以土壤铵态氮、硝态氮、可溶性有机氮及可溶性总氮含量变化为指标,研究了镉胁迫下满江红-鱼腥藻共生体对稻田供氮能力的影响.当土壤中镉浓度为0.3 mg·kg-1时,满江红-鱼腥藻共生体对镉的蓄积量较少,土壤中铵态氮、硝态氮、可溶性有机氮及可溶性总氮含量与对照组无显著差异.当土壤中镉浓度≥1.0 mg·kg-1时,随土壤中镉浓度的增加和处理时间的推移,满江红-鱼腥藻共生体体内的镉含量逐渐增加,土壤中硝态氮、可溶性有机氮及可溶性总氮含量逐渐下降,而铵态氮含量先上升后下降.研究结果表明高浓度的镉胁迫导致满江红-鱼腥藻共生体对土壤的供氮能力显著下降.

  13. 绿萍在稻鸭共作系统中的消长动态及增产效应%Increasing yield and population dynamics of azolla in rice-duck-azolla integrated farming

    Institute of Scientific and Technical Information of China (English)

    甄若宏; 王强盛; 何加骏; 邵明灿; 周建涛; 郑建初; 卞新民

    2009-01-01

    [研究目的]为使适应性强,营养丰富的绿萍在稻田系统中的生态优势得到充分体现,加强绿萍的循环应用,[方法]采用田间小区试验,动态分析了稻鸭萍共作系统的生态结构,绿萍在系统中的生长动态和增产效应.[结果]通过鸭子和绿萍在稻田生态位的添加,使原来的稻田食物链结构趋向复杂化,增加了稻田生态系统的稳定性,有效促进了水稻产量性状的表达和稻米品质的改善.稻鸭萍共作处理的有效穗、千粒重、实际产量分别比对照处理高出7.77%、1.34%和20.03%;整精米率提高4.02%,垩白粒率降低30.24%.随着鸭子的不断成长,对萍的采食量也不断增加,整个共作期间每只鸭可食鲜萍量40.8 kg鸭子在稻田的取食、践踏、嬉戏等行为,利于绿萍的分萍、倒萍,促其生长,绿萍生长后期倒萍速度明显低于稻萍处理.[结论]总之,稻鸭萍共作系统是一项种养相结合,节本增效的生态工程技术.

  14. Effect of BESTKING on controling Azolla%百时清防治红萍的试验

    Institute of Scientific and Technical Information of China (English)

    齐鸣; 李少青

    2010-01-01

    通过喷施和泼浇2种施药方法以及不同浓度喷施,研究百时清药剂对红萍防治效果和水中鱼类的影响.结果表明,10 000 mg·5kg-1喷施方法效果最为理想且安全,不会对鱼类和其它水生植物造成明显的急性致死效应,可以作为红萍防治的参考浓度.

  15. Reactive nitrogen species involved in the rapid root abscission, the stress tolerance mechanism, of the tropical fern Azolla pinata

    OpenAIRE

    Gurung, Sushma; Yamasaki, Hideo

    2009-01-01

    琉球大学21世紀COEプログラム「サンゴ礁島嶼系の生物多様性の総合解析」平成20年度成果発表会(平成21年3月14日開催) 講演・特別講演会場:理系複号棟102号室,ポスター発表会場:琉球大学50周年記念館1F

  16. Differentiation of MIS 9 and MIS 11 in the continental record: vegetational, faunal, aminostratigraphic and sea-level evidence from coastal sites in Essex, UK

    Science.gov (United States)

    Roe, Helen M.; Coope, G. Russell; Devoy, Robert J. N.; Harrison, Colin J. O.; Penkman, Kirsty E. H.; Preece, Richard C.; Schreve, Danielle C.

    2009-11-01

    Multidisciplinary investigations of the vegetational, faunal and sea-level history inferred from the infills of buried channels on the coast of eastern Essex have a direct bearing on the differentiation of MIS 11 and MIS 9 in continental records. New data are presented from Cudmore Grove, an important site on Mersea Island that can be linked to the terrace sequence of the River Thames. The vegetational history has been reconstructed from a pollen sequence covering much of the interglacial represented. The temperate nature of the climate is apparent from a range of fossil groups, including plant remains, vertebrates (especially the rich herpetofauna), molluscs and beetles, which all have strong thermophilous components. The beetle data have been used to derive a Mutual Climatic Range reconstruction, suggesting that mean July temperatures were about 2 °C warmer than modern values for southeast England, whereas mean January temperatures may have been slightly colder. The sea-level history has been reconstructed from the molluscs, ostracods and especially the diatoms, which indicate that the marine transgression occurred considerably earlier in the interglacial cycle than at the neighbouring Hoxnian site at Clacton. There are a number of palynological similarities between the sequence at Cudmore Grove and Clacton, especially the presence of Abies and the occurrence of Azolla filiculoides megaspores. Moreover, both sites have yielded Palaeolithic archaeology, indeed the latter is the type site of the Clactonian (flake-and-core) industry. However, the sites can be differentiated on the basis of mammalian biostratigraphy, new aminostratigraphic data, as well as the differences in the sea-level history. The combined evidence suggests that the infill of the channel at Cudmore Grove accumulated during MIS 9, whereas the deposits at Clacton formed during MIS 11. The infill of a much later channel, yielding non-marine molluscs and vertebrates including Hippopotamus, appears

  17. CHOICE OF MACROPHYTE SUBSTRATE IN THE USE OF DIATOMS AS INDICATORS OF POND WATER QUALITY ASSESSMENT: PRELIMINARY DATA ON THE CASE OF ALALAY POND (COCHABAMBA, BOLIVIA

    Directory of Open Access Journals (Sweden)

    Eduardo A. Morales

    2012-06-01

    Full Text Available Alalay Pond is a 230-hectare, shallow aquatic ecosystem within Cochabamba, the third largest city in Bolivia. With the aim to determine the suitability of epiphytic diatoms for water quality assessment in the pond and to choose a substrate that would hold a representative bioindicator epiphytic community, the macrophytes Schoenoplectus californicus subsp. tatora (Kunth T. Koyama, Typha dominguensis Pers., Myriophyllum verticillatum L. and Azolla filiculoides Lam. were selected and differences in diatom community composition and structure were tested. Diatoms were collected during four sampling campaigns in the March-September, 2011 period, from three stations contiguous to the pelagic zone and prepared for analysis using standard, internationally used protocols. In all, 27 samples were collected from which 28 taxa characteristic of eutrophic environments were identified and 17 others could not be assigned names from the literature. Although many of the species are shared among sampling sites, the epiphytic communities developing at each station were different in structure and composition. Although, there are marked temporal variations in community features on each of the macrophytes, Shannon-Wiener and Pielou indexes, as well as canonical correspondence analysis, showed no marked differences within a single campaign and station among the 4 macrophytes. Shifts in structure and composition are denoted less commonly by species replacement and more often by changes in percent relative abundance of dominant and rare species. Very few species are restricted to a station or seem to show strong preference for a particular substrate. Taking into account growth, structural and ecological characteristics, as well as some phenological features of the macrophytes, S. californicus subsp. tatora and T. dominguensis are the most suitable substrates for water quality assessments in the pond. As demonstrated by multivariate analyses, among-site and time

  18. Growth Performance and Production of Organically Cultured Grass Carp Ctenopharyngodon idella (Val.) Under Mid-Hill Conditions of Meghalaya; North Eastern India

    OpenAIRE

    Majhi, Sullip K.; Das, Anup; Mandal(1), Binoy K.

    2006-01-01

    The aquatic fern Azolla (Azolla caroliniana) can be easily grown organically in North Eastern India due to favorable climatic conditions. This fern was fed to the grass carp, Ctenopharyngodon idella (Val.) as a step towards producing organic fish. In the study, Azolla was found to be a preferred feed by the grass carp and mean daily growth increment was recorded. The statistically paired t-test indicated that the final weight gain of grass carp in Azolla fed ponds were significantly higher (t...

  19. NCBI nr-aa BLAST: CBRC-PCAP-01-1416 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PCAP-01-1416 ref|ZP_03766346.1| hypothetical protein AazoDRAFT_3512 ['Nostoc azolla...e' 0708] gb|EEG03140.1| hypothetical protein AazoDRAFT_3512 ['Nostoc azollae' 0708] ZP_03766346.1 0.56 20% ...

  20. 水田中的有益杂草——满江红%Azolla, a good weed in paddy fields

    Institute of Scientific and Technical Information of China (English)

    裴柳

    2006-01-01

    满江红是一种水生的蕨类植物,是水稻田中的有益杂草.它的有益之处包括:叶片的空腔内有固氮作用的蓝藻与其共生,它通过自己奇特的固氮本领,将空气中的氮气变成氨供满江红使用,使满江红成了赫赫有名的"绿色肥源";抑制其他杂草生长;良好的家禽饲料.

  1. Study on the techniques of rice-azolla-fish ridge culture and its application%垄栽稻萍鱼技术研究与应用

    Institute of Scientific and Technical Information of China (English)

    刘如清; 欧细满; 谢良伍; 肖时运; 夏海鳌; 黄铁平; 文海波; 陈焱国; 黄道友

    2000-01-01

    本文着重阐明了潜育性稻田水稻起垄栽培,形成垄面种稻、水面养萍、水中养鱼的立体种养结构模式,对土壤理化性状、土壤微生物活动和水稻、红萍、鱼类生长的影响及其经济效益.

  2. 红萍在植物治污方面的应用研究进展%The progress in use of Azolla for phytoremediation

    Institute of Scientific and Technical Information of China (English)

    陈坚; 金桂英; 唐龙飞

    2002-01-01

    植物治污是利用植物清除土壤和水中的各类污染物,进行环境治理.水生蕨类植物红萍具有一定的耐污能力,可以通过植物活体的富集作用和干体的物理吸附作用,从溶液中吸附多种重金属元素及通过植物挥发作用,将吸入的硒盐转化为挥发态.在污水中养萍可以去除COD和进行生物脱氮除磷,并设计出相应的养萍盘和生物稳定塘系统进行废水处理;利用红萍干体填充的滤柱可以用于电镀污水中一些重金属元素吸附回收.本文对红萍近年来在植物治污方面的应用和研究进行了描述和展望.

  3. 红萍供氧装置及其试验研究%The Equipment of Using Azolla for O2-supplimentation and its Test

    Institute of Scientific and Technical Information of China (English)

    陈敏; 刘夏石; 刘中柱

    2000-01-01

    以红萍为供O2植物设计研制了进行植物放O2研究的装置.装置是以狗为耗O2动物,设计既考虑到满足红萍生长所需要的光照、温度、湿度、营养以及分萍的要求,又考虑到狗在密封试验舱较长时间所需要解决的食物供应、排泄物的处理以及舱内温度控制等,从而使试验能在一定时间内连续进行.采用该装置进行了红萍放O2试验.结果表明,可选红萍做为供O2植物.

  4. 满江红属系统学研究的新进展%The Progress in Systematic Studies of Azolla

    Institute of Scientific and Technical Information of China (English)

    陈坚; 徐国忠

    2001-01-01

    本文从植物系统学角度对满江红属的研究进展作了全面综述。近年来从细胞研究水平提出了将满江红属重新划分为Azolla和Tetrasporocarpia 2个亚属,经典的三膘(Azolla)和九膘(Rhizosperma)亚属作为新的Subgen.Azolla中的2个组(Section),而原有九膘亚属中的A.nilotica则成为Subgen.Tetrasporocarpia的一个种。遗传育种和同工酶的研究证据对A.mexicana,A.microphylla和A.caroliniana能否在Sect. Azolla中占据3个种的分类地位提出疑问。但要完全解决满江红属的系统学问题,分子生物学技术将是重要手段。由于满江红为蕨-藻共生体,其样本的异质性影响了现有分子标记研究结果的可靠性。

  5. 绿萍的种养及饲喂动物效果研究%Cultivation of Azolla and effect of feeding animal

    Institute of Scientific and Technical Information of China (English)

    李锋涛

    2008-01-01

    绿萍Azollahaimbricata(Rokb)Nakzi又名满江红、红萍、三角藻、红飘等,是满江红科满江红属的一种水生蕨类植物。一般萍体飘浮于水面,是一种优质的水田绿肥植物和兼用的水生饲料植物。大量的研究表明:绿萍鲜嫩多汁纤维素含量少,营养价值较高,味甜,适口性好,是猪、禽、鱼的优质饲料。20世纪70年代,经在辽宁盘锦、辽阳、丹东等地推广引种,现已成为多种畜禽的优质青绿饲料。在吉林省延吉、东申等市、县以及在延边地区也得到推广,在黑龙江省各市、县推广中,也深受畜、禽、鱼场养殖户青睐。

  6. 满江红属5种红萍耐荫性研究%Shade endurance of 5 kinds of Azolla

    Institute of Scientific and Technical Information of China (English)

    徐国忠; 郑向丽; 林觅真; 黄银妹

    2005-01-01

    5种红萍在正常光照(10000 lx)和低光照(3000 lx)下培养10天,进行生物量和多酚氧化酶活性测定,结果表明,五种红萍耐荫性的高低顺序为:卡州满江红、墨西哥满江红、蕨状满江红、小叶满江红和羽叶满江红.

  7. 红萍的光合作用和光抑制的若干探索%A Probe into the Photosynthesis and Photoinhibition of Azolla

    Institute of Scientific and Technical Information of China (English)

    林勇; 郭文杰; 鲁雪华; 刘中柱; 陆培基

    2002-01-01

    通过不同的条件处理,测定了对红萍净光合速率影响,结果表明:采用改变栽培技术变水养萍为湿养萍,以及增加二氧化碳浓度和施加光呼吸抑制剂等技术措施,可显著地提高红萍的净光合速率.

  8. Azolla and Vegetable Experimental Study on the Initial Integration in the CELSS System%CELSS系统中红萍和蔬菜初步整合试验

    Institute of Scientific and Technical Information of China (English)

    杨有泉; 陈敏; 邓素芳

    2010-01-01

    生菜等高等植物在CELSS系统中湿养栽培装置的研制.红萍和蔬菜光合作用效率对比试验结果表明:在不同生育期,生菜的光合作用效率是不一样的;定植5天后生菜植株的光合效率不如红萍,定植15天后生菜植株的光合效率与红萍相当.在现有的湿养栽培装置上试种2~3种不同蔬菜,初步整合试验过程,尚无发现与红萍之间存在有害的不兼容性.

  9. Research on the Reasonable Yield of Azolla in Turtle-raising Pond%甲鱼池放养绿萍合理产量的探讨

    Institute of Scientific and Technical Information of China (English)

    王保辛

    2005-01-01

    甲鱼池放养绿萍是净化水质的有效措施,收获的绿萍可作为草鱼饵料,是一种高效益的生态模式.研究了绿萍放养基数与日产量的关系及绿萍密度对水温的影响,探讨了绿萍的合理产量.

  10. Cytokinin-induced promotion of root meristem size in the fern Azolla supports a shoot-like origin of euphyllophyte roots

    NARCIS (Netherlands)

    de Vries, Jan; Fischer, Angela Melanie; Roettger, Mayo; Rommel, Sophie; Schluepmann, Henriette; Bräutigam, Andrea; Carlsbecker, Annelie; Gould, Sven Bernhard

    2016-01-01

    The phytohormones cytokinin and auxin orchestrate the root meristem development in angiosperms by determining embryonic bipolarity. Ferns, having the most basal euphyllophyte root, form neither bipolar embryos nor permanent embryonic primary roots but rather an adventitious root system. This raises

  11. From phytoaccumulation to post-harvest use of water fern for landfill management.

    Science.gov (United States)

    Song, Uhram; Kim, Dae Won; Waldman, Bruce; Lee, Eun Ju

    2016-11-01

    We examined the potential of Azolla japonica as a remediating plant for leachate channels and post-accumulation use as fertilizer for landfill slope. The harvested biomass of Azolla after one month grown in leachate was 254% that of the initial biomass and the predicted annual harvestable biomass of Azolla using a growth model was 32 times that of the initial biomass. Na, Fe, Mn, Mg, and P were accumulated in Azolla at very high concentrations. Such rapid increase of biomass and high accumulation rates suggest that this plant could be an excellent remediating plant. The post-harvest use of Azolla as compost was studied for the management and use of phytoaccumulating Azolla. Metal contents of Azolla compost were below permissible limits for co-composting material. Nitrogen, organic matter, P, and Mg content of the Azolla compost improved the soil condition of the landfill and enhanced ecophysiological responses of the plants. The application of Azolla compost can improve management of sanitary landfills, including the restoration of vegetation. Considering its ease of harvesting, high accumulation rates, harvestable biomass and suitability for composting, Azolla can provide a suitable solution for sustainable management of leachate channels and landfill slopes. PMID:27454092

  12. From phytoaccumulation to post-harvest use of water fern for landfill management.

    Science.gov (United States)

    Song, Uhram; Kim, Dae Won; Waldman, Bruce; Lee, Eun Ju

    2016-11-01

    We examined the potential of Azolla japonica as a remediating plant for leachate channels and post-accumulation use as fertilizer for landfill slope. The harvested biomass of Azolla after one month grown in leachate was 254% that of the initial biomass and the predicted annual harvestable biomass of Azolla using a growth model was 32 times that of the initial biomass. Na, Fe, Mn, Mg, and P were accumulated in Azolla at very high concentrations. Such rapid increase of biomass and high accumulation rates suggest that this plant could be an excellent remediating plant. The post-harvest use of Azolla as compost was studied for the management and use of phytoaccumulating Azolla. Metal contents of Azolla compost were below permissible limits for co-composting material. Nitrogen, organic matter, P, and Mg content of the Azolla compost improved the soil condition of the landfill and enhanced ecophysiological responses of the plants. The application of Azolla compost can improve management of sanitary landfills, including the restoration of vegetation. Considering its ease of harvesting, high accumulation rates, harvestable biomass and suitability for composting, Azolla can provide a suitable solution for sustainable management of leachate channels and landfill slopes.

  13. Research on the Effects of Space Induction of Sporocarps of Azolla--Ⅱ Effect of Space Conditions on the Shade Tolerance of Different Species of Azolla%满江红孢子果空间诱变效应的研究——Ⅱ空间条件对不同品系满江红耐荫性的影响

    Institute of Scientific and Technical Information of China (English)

    鲁雪华; 卞祖良; 郭文杰; 陈敏

    2002-01-01

    研究了高空条件对不同品系满江红孢子果诱变的效应,结果表明,高空处理对不同品系满江红孢子果的生长发育有明显的影响[1],在生物学观察测定的基础上引入生化测定方法,探讨不同光照条件下经高空处理与留地对照满江红3个品系体内多酚氧化酶(PPO)活性变化趋势.其结果再次表明,光照影响各满江红品系的生长速率,随着光照强度的减弱,各品系的生长速率都下降,但在低光照下,经高空处理的19 H品系其体内PPO活性明显高于其它品系,同样的也比留地对照的同一品系19 CK高13.8 %,充分说明经高空处理后的19 H品系耐荫性得到明显提高,初步论证了以高空诱变作为满江红品种改良手段是可行的.

  14. 满江红鱼腥藻与其宿主的遗传多样性和协同性的RAPD分析%The Genetic Diversity and Homology of Anabaena azollae and its Host Plant (Azolla) Based on Rapd Analysis

    Institute of Scientific and Technical Information of China (English)

    陈坚; 郑伟文; 徐国忠; 宋铁英; 唐龙飞

    2002-01-01

    从16个代表不同种属或地域来源的满江红样本中分离出共生藻并通过处理获得无藻的满江红宿主,对二者同步进行了RAPD扩增,分别得到了大量DNA多态片段.通过建立满江红鱼腥藻及其宿主的UPGMA聚类关系图,看出二者在遗传分支上存在着一定程度的协同对应关系.但在种内的不同品系间,这种协同性有所减少,发现有的品系的共生藻发生了明显的变异.

  15. The omnipresent water fern

    NARCIS (Netherlands)

    Nierop, K.G.J.; Speelman, E.N.; de Leeuw, J.W.; Reichart, G.J.

    2011-01-01

    Several studies have reported the presence of large amounts of lignin in ubiquitously occurring species of the freshwater fern Azolla. Molecular analysis using flash pyrolysis and thermally assisted hydrolysis and methylation shows, however, that neither the leaves nor the roots of Azolla contain li

  16. NCBI nr-aa BLAST: CBRC-STRI-01-1723 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-1723 ref|ZP_03763981.1| glycosyl transferase family 2 ['Nostoc azollae...' 0708] gb|EEG05547.1| glycosyl transferase family 2 ['Nostoc azollae' 0708] ZP_03763981.1 0.25 25% ...

  17. Soil Fertility Status on Organic Paddy Experiment

    Directory of Open Access Journals (Sweden)

    Mujiyo

    2015-07-01

    Full Text Available The study aims to determine fertility status of the soil after organic paddy experiments using kinds and doses of organic fertilizers. Experiment was conducted at greenhouse laboratory in Faculty of Agriculture Sebelas Maret University Surakarta. Experimental design used completely randomized design with 9 kinds of treatment was replicated 3 times. Experiments were the use of cow manure, Azolla fertilizer, Azolla inoculum and its combinations that are based on fulfilling nutrient requirements of 120 kg N ha-1. Result shows that the use of cow manure, Azolla fertilizers and Azolla inoculum had no effect on changes of soil fertility status. Soil fertility status was not significantly correlated with cow manure (0,16ns, Azolla fertilizer (0,26ns and Azolla inoculum (0,16ns. Average of final soil fertility status included fertile category, which was similar as the initial soil fertility status. Average of final soil properties of treatment but nevertheless was relatively higher than in no treatment, indicating the use of cow manure, Azolla fertilizer, Azolla inoculum and its combinations had greater impact to soil properties. Cow manure despite increased available K2O and dry grain, but it did not significantly increase the soil fertility status from fertile to very fertile. This was presumably due to the relatively short experiment period, only one planting season had not given significant effect to soil properties. Implication of this study is the use of cow manure, Azolla fertilizer, Azolla inoculum and its combinations although did not increase the soil fertility status but could maintain soil fertility status as the initial conditions before planting.

  18. 蕨类杂草用满江红进行除草活性生测方法的研究%Research of a new bioassay method using Azolla imbricate as indicator during fern control

    Institute of Scientific and Technical Information of China (English)

    陈燕芳; 丁伟; 张永强

    2007-01-01

    蕨类植物杂草是一类很难治理的杂草,防除蕨类杂草目前还没有合适的药剂,更没有蕨类植物除草剂室内生物测定的方法.本研究选用满江红作为指示植物,以探索一种蕨类植物除草剂室内生物测定的方法.结果表明,对试验药剂草甘膦和克无踪反应灵敏,其中,草甘膦对其作用的最佳观察时间为3~7 d,克无踪对其作用的最佳观察时间为1~2 d.说明满江红法具有一定的可行性,满江红是作为蕨类植物除草剂室内活性筛选和评价的一个重要的指示植物.

  19. THE CLONE OF ANABAENA AZOLLAE GLNA PROMOTER AND ACTIVITY EXPRESSION IN TRANSGENIC TOBACCO%满江红鱼腥藻glnA启动区的克隆及在烟草中的活性表达

    Institute of Scientific and Technical Information of China (English)

    刘祥林; 印莉萍; 吴燕川; 高志环; 吴晓强

    2000-01-01

    利用PCR技术获得满江红鱼腥藻glnA启动区,经克隆测序后构成谷氨酰胺合成酶基因启动子驱动的GUS基因表达载体.用基因枪转化,将表达载体导入烟草中,在其叶片和茎中检测到GUS活性,而在烟草根中未见表达.实验结果对真核生物与原核生物间基因表达调控、转录因子识别的研究以及构建实用载体具有一定价值.

  20. Research Progress on Utilization of Azolla and Its Potential Contribution to Environmental Restoration%满江红资源化利用及对环境修复作用的研究进展

    Institute of Scientific and Technical Information of China (English)

    万合锋; 龙朝波; 兰晨; 龙云川; 秦华军; 武玉祥; 袁果

    2015-01-01

    满江红Azolla是常见的“蕨-藻”共生的水生植物,具有较高的经济价值和生态价值.通常作为绿肥、饲料和污水治理植物用于农业生产与综合开发,并作为水域生态修复重要的生物种质资源,尤其是用于富营养化湖泊、池塘、沟渠等水体的氮磷去除和重金属吸附转移等方面.本文总结了满江红的生物学特征、资源化利用和生态环境修复方面的应用及成效,分析了满江红在开发利用研究中的诸多优势,并对其潜在的开发利用价值作了展望.

  1. 满江红九膘亚属种雄性育性的初步调查研究%A Preliminary Investigation on Male Fertility of Azolla Species in Subgenus Rhizosperma

    Institute of Scientific and Technical Information of China (English)

    金桂英; 陈坚; 唐龙飞

    2001-01-01

    将满江红九膘亚属3个种小孢子育性调查结果分成两大类.第一类特点是小孢子败育程度严重,全败育或近全败育;第二类特点为小孢子败育程度轻或较轻,总败育率变化幅度大.讨论了影响小孢子育性因素——遗传和外界环境条件.另外,就大型小孢子的发现,推测未减数配子可能是形成多倍体途径之一.

  2. 废电池对水质的影响及满江红的净化效果%Effect of Wasted Battery on Water Quality and Purified Efficiency of Azolla imbricata

    Institute of Scientific and Technical Information of China (English)

    袁丽水

    2000-01-01

    @@ 1 前言 废电池对水生生物的危害很大.20世纪30年代,日本某小镇由于常把用过的废电池随手扔在井水边,久而久之,电池中的有毒物质污染了井水,人们饮用了含有大量有毒物质的水,引起中毒,造成多人发疯. 废电池含有哪些有害、有毒物质,对水生生物的危害究竟如何,怎样净化废电池对水质的污染等,我们通过实验进行了一些探讨.

  3. 稻-萍-鱼体系对稻田土壤环境的影响%Effect of rice-azolla-fish system on soil environment of rice field.

    Institute of Scientific and Technical Information of China (English)

    黄毅斌; 翁伯奇; 唐建阳; 刘中柱

    2001-01-01

    稻-萍-鱼体系的基本技术是在单一以水稻为主体的生物群体中加入红萍和鱼类,通过对红萍和鱼的人工调控而影响整个稻田生态体系.在综合技术作用下,稻-萍-鱼体系中可混养多种鱼类,产量达4000~9800kg/hm2;在少用50%~60%化肥、30%~50%农药、鱼沟及鱼坑占地10%~15%情况下,水稻产量比常规种稻略增,且土壤有机质、全N、全P等上升15.6%~38.5%,水稻病虫草害发生下降40.8%~99.5%,土壤甲烷(CH4)排放量减少34.6%,显著改善了稻田生态环境.

  4. Techniques for Raising Ducks with Azolla in the Fields of Single Cropping Hybrid Rice%单季杂交稻田稻鸭萍共育技术初步总结

    Institute of Scientific and Technical Information of China (English)

    蓝月相; 孙蔚丽; 董益坤; 陈水清

    2002-01-01

    稻鸭萍共育技术是以种稻为基础,家鸭野养为特点,将水稻的生育特点、病虫害发生规律和鸭的生活习性及水生浮游生物消涨规律有机结合的种养方式,可使农民达到增产增收的目的.

  5. Cultivation of introduced forage plant - Azolla filiculoidea in Qingyang loess plateau, Gansu, China%绿色饲用植物--细绿萍在黄土高原引种栽培

    Institute of Scientific and Technical Information of China (English)

    李峻成; 高崇岳; 王凌云; 姬永莲; 赵明轩; 孟宪政

    2001-01-01

    原产于美国的细绿萍70年代在西方国家广泛应用,中国科学院引进我国后,经过多年的驯化已经适宜我国北方地区养殖,作为优质的家畜饲料引进陇东黄土高原栽培、推广,具有产量高、宜推广,经济效益、社会效益、生态效益显著的特点.

  6. 槐叶萍、浮萍、满江红对水体中镉离子的吸收研究%Study on Ability of Salvinia natans, Lemna minor and Azolla imbricata to Cumulate Cd2+

    Institute of Scientific and Technical Information of China (English)

    刘俊; 杨阿喜; 张晓燕; 金根娣

    2010-01-01

    在实验室水培条件下,研究了槐叶萍、满江红、浮萍等水生植物对Cd2+的累积吸收能力.用原子吸收分光光度法对三种植物的水培液中Cd2+的浓度进行了测定.结果表明Cd2+在2~20mg·L-1的浓度范围内,三种植物对Cd2+的累积吸收量随着浓度的增加而增加,槐叶萍累积吸收能力大于满江红,满江红优于浮萍;在40~100mg·L-1的浓度范围内,三种植物对Cd2+的累积吸收量则随着浓度的递增而大幅降低,槐叶萍对Cd2+的去除效果较其它两种植物为佳.因此,在治理轻度镉污染水体时,三种植物均是较好的备选植物,而在治理重度镉污染水体时,应首选满江红.

  7. Mitigating yield-scaled greenhouse gas emissions through combined application of soil amendments: A comparative study between temperate and subtropical rice paddy soils

    International Nuclear Information System (INIS)

    Effects of different soil amendments were investigated on methane (CH4) and nitrous oxide (N2O) emissions, global warming potential (GWP) and yield scaled GWPs in paddy soils of Republic of Korea, Japan and Bangladesh. The experimental treatments were NPK only, NPK + fly ash, NPK + silicate slag, NPK + phosphogypsum(PG), NPK + blast furnace slag (BFS), NPK + revolving furnace slag (RFS), NPK + silicate slag (50%) + RFS (50%), NPK + biochar, NPK + biochar + Azolla-cyanobacteria, NPK + silicate slag + Azolla-cyanobacteria, NPK + phosphogypsum (PG) + Azolla-cyanobacteria. The maximum decrease in cumulative seasonal CH4 emissions was recorded 29.7% and 32.6% with Azolla-cyanobacteria plus phospho-gypsum amendments in paddy soils of Japan and Bangladesh respectively, followed by 22.4% and 26.8% reduction with silicate slag plus Azolla-cyanobacteria application. Biochar amendments in paddy soils of Japan and Bangladesh decreased seasonal cumulative N2O emissions by 31.8% and 20.0% respectively, followed by 26.3% and 25.0% reduction with biochar plus Azolla-cyanobacteria amendments. Although seasonal cumulative CH4 emissions were significantly increased by 9.5–14.0% with biochar amendments, however, global warming potentials were decreased by 8.0–12.0% with cyanobacterial inoculation plus biochar amendments. The maximum decrease in GWP was calculated 22.0–30.0% with Azolla-cyanobacteria plus silicate slag amendments. The evolution of greenhouse gases per unit grain yield (yield scaled GWP) was highest in the NPK treatment, which was decreased by 43–50% from the silicate slag and phosphogypsum amendments along with Azolla-cyanobacteria inoculated rice planted soils. Conclusively, it is recommended to incorporate Azolla-cyanobacteria with inorganic and organic amendments for reducing GWP and yield scaled GWP from the rice planted paddy soils of temperate and subtropical countries. - Highlights: • Azolla-cyanobacteria with organic and inorganic amendments in paddy

  8. Mitigating yield-scaled greenhouse gas emissions through combined application of soil amendments: A comparative study between temperate and subtropical rice paddy soils

    Energy Technology Data Exchange (ETDEWEB)

    Ali, Muhammad Aslam, E-mail: litonaslam@yahoo.com [Dept. of Environmental Science, Bangladesh Agricultural University, Mymensingh 2202 (Bangladesh); Dept. of Agricultural Chemistry, Gyeongsang National University, Jinju (Korea, Republic of); Division of Environmental Horticulture, Chiba University, Matsudo, Chiba 271-8510 (Japan); Kim, P.J., E-mail: pjkim@nongae.gsnu.ac.kr [Dept. of Agricultural Chemistry, Gyeongsang National University, Jinju (Korea, Republic of); Inubushi, K. [Division of Environmental Horticulture, Chiba University, Matsudo, Chiba 271-8510 (Japan)

    2015-10-01

    Effects of different soil amendments were investigated on methane (CH{sub 4}) and nitrous oxide (N{sub 2}O) emissions, global warming potential (GWP) and yield scaled GWPs in paddy soils of Republic of Korea, Japan and Bangladesh. The experimental treatments were NPK only, NPK + fly ash, NPK + silicate slag, NPK + phosphogypsum(PG), NPK + blast furnace slag (BFS), NPK + revolving furnace slag (RFS), NPK + silicate slag (50%) + RFS (50%), NPK + biochar, NPK + biochar + Azolla-cyanobacteria, NPK + silicate slag + Azolla-cyanobacteria, NPK + phosphogypsum (PG) + Azolla-cyanobacteria. The maximum decrease in cumulative seasonal CH{sub 4} emissions was recorded 29.7% and 32.6% with Azolla-cyanobacteria plus phospho-gypsum amendments in paddy soils of Japan and Bangladesh respectively, followed by 22.4% and 26.8% reduction with silicate slag plus Azolla-cyanobacteria application. Biochar amendments in paddy soils of Japan and Bangladesh decreased seasonal cumulative N{sub 2}O emissions by 31.8% and 20.0% respectively, followed by 26.3% and 25.0% reduction with biochar plus Azolla-cyanobacteria amendments. Although seasonal cumulative CH{sub 4} emissions were significantly increased by 9.5–14.0% with biochar amendments, however, global warming potentials were decreased by 8.0–12.0% with cyanobacterial inoculation plus biochar amendments. The maximum decrease in GWP was calculated 22.0–30.0% with Azolla-cyanobacteria plus silicate slag amendments. The evolution of greenhouse gases per unit grain yield (yield scaled GWP) was highest in the NPK treatment, which was decreased by 43–50% from the silicate slag and phosphogypsum amendments along with Azolla-cyanobacteria inoculated rice planted soils. Conclusively, it is recommended to incorporate Azolla-cyanobacteria with inorganic and organic amendments for reducing GWP and yield scaled GWP from the rice planted paddy soils of temperate and subtropical countries. - Highlights: • Azolla-cyanobacteria with organic and

  9. The recovery of polyphenols from olive mill waste using two adsorbing vegetable matrices.

    Science.gov (United States)

    Ena, Alba; Pintucci, Cristina; Carlozzi, Pietro

    2012-02-20

    Olive mill wastewater (OMW) is considered one of the most pollutive waste materials in the Mediterranean basin. However, its phenolic fraction should be recovered, since it has been shown to have incredible benefits for health. In the present study, the adsorbent and desorbent capacities of Azolla and granular activated carbon (GAC) were investigated. The GAC was found to be more efficient than Azolla in both the adsorption and the desorption of phenols. The total characterization of two powder products obtained from Azolla and GAC desorption is reported, together with their antioxidant and antiradical activities. In the Azolla powder product, total polyphenols were more than twice as numerous as those found in the GAC powder product. The GAC powder contained hydroxytyrosol in concentrations that were 3.5 times higher than those of Azolla. On the other hand, both powder products showed great antiradical activities: the IC₅₀ was found to be 102 mg ml⁻¹ for the Azolla and 199 mg ml⁻¹ for the GAC powders respectively. The oxygen radical absorbance capacity was very high: 4097 μmol TE g⁻¹ Azolla powder product and 1277 μmol TE g⁻¹ of GAC powder products. PMID:21740933

  10. Mitigating yield-scaled greenhouse gas emissions through combined application of soil amendments: A comparative study between temperate and subtropical rice paddy soils.

    Science.gov (United States)

    Ali, Muhammad Aslam; Kim, P J; Inubushi, K

    2015-10-01

    Effects of different soil amendments were investigated on methane (CH4) and nitrous oxide (N2O) emissions, global warming potential (GWP) and yield scaled GWPs in paddy soils of Republic of Korea, Japan and Bangladesh. The experimental treatments were NPK only, NPK+fly ash, NPK+silicate slag, NPK+phosphogypsum(PG), NPK+blast furnace slag (BFS), NPK+revolving furnace slag (RFS), NPK+silicate slag (50%)+RFS (50%), NPK+biochar, NPK+biochar+Azolla-cyanobacteria, NPK+silicate slag+Azolla-cyanobacteria, NPK+phosphogypsum (PG)+Azolla-cyanobacteria. The maximum decrease in cumulative seasonal CH4 emissions was recorded 29.7% and 32.6% with Azolla-cyanobacteria plus phospho-gypsum amendments in paddy soils of Japan and Bangladesh respectively, followed by 22.4% and 26.8% reduction with silicate slag plus Azolla-cyanobacteria application. Biochar amendments in paddy soils of Japan and Bangladesh decreased seasonal cumulative N2O emissions by 31.8% and 20.0% respectively, followed by 26.3% and 25.0% reduction with biochar plus Azolla-cyanobacteria amendments. Although seasonal cumulative CH4 emissions were significantly increased by 9.5-14.0% with biochar amendments, however, global warming potentials were decreased by 8.0-12.0% with cyanobacterial inoculation plus biochar amendments. The maximum decrease in GWP was calculated 22.0-30.0% with Azolla-cyanobacteria plus silicate slag amendments. The evolution of greenhouse gases per unit grain yield (yield scaled GWP) was highest in the NPK treatment, which was decreased by 43-50% from the silicate slag and phosphogypsum amendments along with Azolla-cyanobacteria inoculated rice planted soils. Conclusively, it is recommended to incorporate Azolla-cyanobacteria with inorganic and organic amendments for reducing GWP and yield scaled GWP from the rice planted paddy soils of temperate and subtropical countries. PMID:26011612

  11. Changes in symbiotic and associative interrelations in a higher plant-bacterial system during space flight

    Science.gov (United States)

    Kordyum, V. A.; Man'ko, V. G.; Popova, A. F.; Shcherbak, O. H.; Mashinsky, A. L.; Nguen-Hgue-Thyok

    The miniature cenosis consisting of the water fern Azolla with its associated symbiotic nitrogen-fixing cyanobacterium Anabaena and the concomitant bacteria was investigated. Ecological closure was shown to produce sharp quantitative and qualitative changes in the number and type of concomitant bacteria. Changes in the distribution of bacterial types grown on beef-extract broth after space flight were recorded. Anabaena azollae underwent the most significant changes under spaceflight conditions. Its cell number per Azolla biomass unit increased substantially. Thus closure of cenosis resulted in a weakening of control over microbial development by Azolla. This tendency was augmented by spaceflight factors. Reduction in control exerted by macro-organisms over development of associated micro-organisms must be taken into account in constructing closed ecological systems in the state of weightlessness.

  12. Protein (Cyanobacteria): 381540 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available YP_003720505.1 1117:24359 1161:2514 1162:2837 264688:721 1164:721 551115:721 DNA-di...rected RNA polymerase subuint gamma 'Nostoc azollae' 0708 MRSAQSNQFDYVKIGLASPERIRQWGERTLPNGQVVGEVTKPETINYRTL

  13. Azolla—A Model Organism for Plant Genomic Studies

    Institute of Scientific and Technical Information of China (English)

    Yin-LongQiu; JunYu

    2003-01-01

    The aquatic ferns of the genus Azolla are nitrogen-fixing plants that have great potentials in agricultural production and environmental conservation.Azolla in many aspects is qualified to serve as a model organism for genomic studies because of its importance in agriculture,its unique position in plant evolution,its symbiotic relationship with the N2-fixing cyanobacterium,Anabaena azollae,and its moderate-sized genome.The goals of this genome project are not only to understand the biology of the Azolla genome to promote its applications in biological research and agriculture practice but also to gain critical insights about evolution of plant genomes.Together with the strategic and technical improvement as well as cost reduction of DNA sequencing,the deciphering of their genetic code is imminent.

  14. Soil Fertility Status on Organic Paddy Experiment

    OpenAIRE

    Mujiyo; Bambang Hendro Sunarminto; Eko Hanudin; Jaka Widada

    2015-01-01

    The study aims to determine fertility status of the soil after organic paddy experiments using kinds and doses of organic fertilizers. Experiment was conducted at greenhouse laboratory in Faculty of Agriculture Sebelas Maret University Surakarta. Experimental design used completely randomized design with 9 kinds of treatment was replicated 3 times. Experiments were the use of cow manure, Azolla fertilizer, Azolla inoculum and its combinations that are based on fulfilling nutrient requirements...

  15. Nuevo registro de greenidea ficicola takahashi (hemiptera: sternorrhyncha:aphididae) en guayabo psidium guajava (myrtaceae) en antioquia, colombia.

    OpenAIRE

    David Giraldo, Rubén Darío; Quiroz Gamboa, Jhon Albeiro; Yepes Rodríguez, Francisco; SmithPardo, Allan Henry

    2011-01-01

    Se encuentra por primera vez en el departamento de Antioquia (Colombia) y asociado al guayabo (Psidium guajava L.), la especie Greenidea ficicola Takahashi ( Hemiptera: Sternorrhyncha: Aphididae) colectada durante muestreos intensivos realizados durante el primer semestre de 2008 en el Valle de Aburrá y municipios aledaños. Dicha especie de áfido también fue colectada en falso laurel (Ficus benjamina). Dada la importancia que tienen los frutos de guayabo en la alimentación humana y de los ...

  16. NUEVO REGISTRO DE Greenidea ficicola Takahashi (Hemiptera: Sternorrhyncha: Aphididae) EN GUAYABO Psidium guajava (Myrtaceae) EN ANTIOQUIA, COLOMBIA NEW REGISTRATION OF Greenidea ficicola Takahashi (Hemiptera: Sternorrhyncha: Aphididae) IN GUAVA Psidium guajava (Myrtaceae) IN ANTIOQUIA, COLOMBIA

    OpenAIRE

    Rubén Darío David Giraldo; Jhon Albeiro Quiroz Gamboa; Francisco Yepes Rodríguez; Allan Henry Smith Pardo

    2009-01-01

    Se encuentra por primera vez en el departamento de Antioquia (Colombia) y asociado al guayabo (Psidium guajava L.), la especie Greenidea ficicola Takahashi ( Hemiptera: Sternorrhyncha: Aphididae) colectada durante muestreos intensivos realizados durante el primer semestre de 2008 en el Valle de Aburrá y municipios aledaños. Dicha especie de áfido también fue colectada en falso laurel (Ficus benjamina). Dada la importancia que tienen los frutos de guayabo en la alimentación humana y de los ani...

  17. Nuevos bivalvos para el Perú en Bahía Independencia

    Directory of Open Access Journals (Sweden)

    Oswaldo Cornejo

    2013-06-01

    Full Text Available Se registran por primera vez para el Perú las siguientes especies de bivalvos Nucula (Nucula pisum Sowerby I, 1833, y Lyonsia delicata Marincovich, 1973, colectadas en Bahía Independencia, Pisco, Perú, en el 2002.

  18. Episodic fresh surface waters in the Eocene Arctic Ocean

    Science.gov (United States)

    Brinkhuis, Henk; Schouten, Stefan; Collinson, Margaret E.; Sluijs, Appy; Damsté, Jaap S. Sinninghe; Dickens, Gerald R.; Huber, Matthew; Cronin, Thomas M.; Onodera, Jonaotaro; Takahashi, Kozo; Bujak, Jonathan P.; Stein, Ruediger; van der Burgh, Johan; Eldrett, James S.; Harding, Ian C.; Lotter, André F.; Sangiorgi, Francesca; Cittert, Han Van Konijnenburg-Van; de Leeuw, Jan W.; Matthiessen, Jens; Backman, Jan; Moran, Kathryn; Expedition 302 Scientists

    2006-06-01

    It has been suggested, on the basis of modern hydrology and fully coupled palaeoclimate simulations, that the warm greenhouse conditions that characterized the early Palaeogene period (55-45Myr ago) probably induced an intensified hydrological cycle with precipitation exceeding evaporation at high latitudes. Little field evidence, however, has been available to constrain oceanic conditions in the Arctic during this period. Here we analyse Palaeogene sediments obtained during the Arctic Coring Expedition, showing that large quantities of the free-floating fern Azolla grew and reproduced in the Arctic Ocean by the onset of the middle Eocene epoch (~50Myr ago). The Azolla and accompanying abundant freshwater organic and siliceous microfossils indicate an episodic freshening of Arctic surface waters during an ~800,000-year interval. The abundant remains of Azolla that characterize basal middle Eocene marine deposits of all Nordic seas probably represent transported assemblages resulting from freshwater spills from the Arctic Ocean that reached as far south as the North Sea. The termination of the Azolla phase in the Arctic coincides with a local sea surface temperature rise from ~10°C to 13°C, pointing to simultaneous increases in salt and heat supply owing to the influx of waters from adjacent oceans. We suggest that onset and termination of the Azolla phase depended on the degree of oceanic exchange between Arctic Ocean and adjacent seas.

  19. The Similarities and Differences of the Guzheng Concerto "Lin'an grudge" with Nguyen Concerto "Azolla"%浅谈古筝协奏曲《临安遗恨》与中阮协奏曲《满江红》的异同

    Institute of Scientific and Technical Information of China (English)

    刘洁

    2013-01-01

    古筝协奏曲《临安遗恨》自面世至今一直广受好评,此曲充分发挥了古筝这种大性情的乐器雄浑的气势,出色地表现出抗金英雄岳飞“壮志饥餐胡虏肉,笑谈渴饮匈奴血”的雄心壮志、快意恩仇,同样也表现出了对奸臣当道,报国无门现实的悲愤、哀痛与无可奈何.随着对音乐学习的深入,不断接触新的优秀曲目,笔者对中阮协奏曲《满江红》与古筝协奏曲《临安遗恨》的曲目构思及其表现手法的异同也多了一些逐步深化的理解.

  20. 红萍对受控密闭系统中O2-CO2浓度变化影响研究初报%Effects of Azolla on the change of O2-CO2 concentration under controlled airtight system

    Institute of Scientific and Technical Information of China (English)

    陈敏; 卞祖良; 张朝阳; 刘晖; 陈炳焕

    1999-01-01

    建立受控密闭系统,研究在动物和红萍共存情况下,系统中O2-CO2浓度的变化规律.红萍的光合作用与狗的呼吸作用相辅相成,使系统中O2-CO2浓度从急骤变化达到基本保持平衡.由于系统中O2浓度大于16%,CO2浓度小于2%,狗生活正常;而仅有动物存在情况下,狗消耗系统中O2并释放CO2,很快出现严重缺O2和CO2中毒症状.

  1. Biomarker Constraints on Arctic Surface Water Conditions During the Middle Eocene

    Science.gov (United States)

    Speelman, E. N.; Reichart, G.; Brinkhuis, H.; Sinninghe Damste, J. S.; de Leeuw, J. M.; van Kempen, M.

    2007-12-01

    Through analyses of unique microlaminated sediments of Arctic drill cores, recovered from the Lomonosov Ridge in the central Arctic Ocean during Integrated Ocean Drilling Program (IODP) Expedition 302, it has been shown that enormous quantities of the free floating freshwater fern \\textit {Azolla} grew and reproduced in situ in the Arctic Ocean during the middle Eocene (Brinkhuis et al., Nature, 2006).The presence of the freshwater fern Azolla, both within the Arctic Basin and in all Nordic seas, suggests that at least the sea surface waters were frequently dominated by fresh- to brackish water during an interval of at least 800 kyr. However, to which degree the Arctic Basin became fresh and what the consequences of these enormous Azolla blooms were for regional and global nutrient cycles is still largely unknown. Comparing samples of extant Azolla, including its nitrogen fixing symbionts, with samples from the Arctic Azolla interval revealed the presence of a group of highly specific biomarkers. These biomarkers are closely related to similar organic compounds that have been suggested to play a crucial role in the biogeochemistry of nitrogen fixing bacteria. This finding, therefore, potentially implies that this symbioses dates back to at least the middle Eocene. Furthermore, this particular symbiosis was probably crucial in triggering basin wide Azolla blooms. We now aim to measure compound specific stable hydrogen isotope values of these biomarkers which should provide insight into the degree of mixing between high salinity (isotopically heavy) deeper and low salinity surface water (isotopically light). The results of these compound specific isotope analyses will be extrapolated using calibrations from controlled growth experiments and subsequently evaluated using climate modeling experiments.

  2. Pemanfaatan Limbah Tandan Kosong Kelapa Sawit Dan Azola Menjadi Kompos Pupuk Tablet

    OpenAIRE

    Ahmad, Dahyar

    2010-01-01

    It has been conducted a research on the uses of palm-oil empty bunches (TKKS) and the azolla to become compost fertilizer on tablet. The research was done by composting each TKKS and azolla for 30 days there using EM4 (Effective micro-organism 4) activator. In order to measure the content of manure element C, N, P and K compost there were conducted an analysis during the compost taking place with the time variety of 0, 5, 10, 15, 20, 25, and 30 days. By the compost tablet analysis C/N comp...

  3. Biosolubilization of uranyl ions in uranium ores by hydrophyte plants

    International Nuclear Information System (INIS)

    This paper investigated the bioleaching of uranyl ions from uranium ores, in aqueous medium by hydrophyte plants: Lemna minor, Azolla caroliniana and Elodea canadensis under different experimental conditions. The oxidation of U(IV) to U(VI) species was done by the atomic oxygen generated in the photosynthesis process by the aquatic plants in the solution above uranium ores. Under identical experimental conditions, the capacity of bioleaching of uranium ores decreases according to the following series: Lemna minor > Elodea canadensis > Azolla caroliniana. The results of IR spectra suggest the possible use of Lemna minor and Elodea canadensis as a biological decontaminant of uranium containing wastewaters. (author)

  4. A Giant Arctic Freshwater Pond at the end of the Early Eocene; Implications for Ocean Heat Transport and Carbon Cycling

    Science.gov (United States)

    Brinkhuis, H.; Schouten, S.; Collinson, M. E.; Sluijs, A.; Sinninghe-Damste, J. S.; Dickens, G. R.; Huber, M.; Cronin, T. M.; Bujak, J. P.; Stein, R.; Eldrett, J. S.; Harding, I. C.; Sangiorgi, F.

    2005-12-01

    In the last decades remains of the free-floating, fresh water fern Azolla have been found in unusually high abundances in basal middle Eocene (~48.5 Ma) marine sediments deposited in all Nordic seas. While generally taken to signal some `freshwater input', their source and significance were not determined. Through palynological and organic geochemical analyses of unique cores obtained from unprecedented Arctic Ocean drilling (IODP 302 - ACEX) we show that the brackish surface conditions that prevailed in the Arctic Ocean through the late Paleocene and early Eocene culminated in the deposition of laminated organic rich deposits yielding huge amounts of remains of Azolla. This, plus e.g., low diversity dinoflagellate assemblages, and concomitant low BIT values, indicates in-situ Azolla growth, and that the surface of the Arctic Ocean episodically resembled a giant fresh water pond over an interval altogether lasting ~800,000 years. The Arctic Basin thus constituted the main source of the freshwater pulses found elsewhere, reaching as far south as the southern North Sea.TEX86-derived surface temperatures were 13-14°C before and after the Azolla interval and only 10°C during the event, which may be related to obstruction of pole ward ocean heat transport and/or increased carbon burial.

  5. Alternative energy from agriculture: biological conversion and recycling

    Energy Technology Data Exchange (ETDEWEB)

    Massantini, F.; Caporali, F.; Masoni, A.

    1980-01-01

    A review on producing methanol and ethanol from agricultural products (sugar beet, sweet sorghum sugar cane, etc.) and biogas from aquatic plants (Eichhornia crassipes) and algae (Azolla, Chlorella) is given. Anaerobic fermentation of liquid manure and sewage, straw, distillery residues, and other organic wastes is also covered. (Refs. 42).

  6. Effect of three polycyclic aromatic hydrocarbons on nodulation of Rhizobium tropici CIAT 899 on Phaseolus vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez-Paredes, Y.; Ferrera-Cerrato, R.; Alarcon, A.

    2009-07-01

    Polycyclic aromatic hydrocarbons (PAH) are ubiquitous organic pollutants that are considered toxic and carcinogenic compounds to living organisms. There us scarce information about the effect of PAH on symbiotic systems such as Azolla-Anabaena, arbuscular mycorrhizal fungi-plants, or legume-rhizobia. (Author)

  7. Modeling the influence of a reduced equator-to-pole sea surface temperature gradient on the distribution of water isotopes in the Early/Middle Eocene

    NARCIS (Netherlands)

    Speelman, E.N.; Sewall, J.O.; Noone, D.; Huber, M.; Heydt, A.S. von der; Sinninghe Damsté, J.S.; Reichart, G.-J.

    2010-01-01

    Proxy-based climate reconstructions suggest the existence of a strongly reduced equator-to-pole temperature gradient during the Azolla interval in the Early/Middle Eocene, compared to modern. Changes in the hydrological cycle, as a consequence of a reduced temperature gradient, are expected to be re

  8. Arabidopsis CDS blastp result: AK060074 [KOME

    Lifescience Database Archive (English)

    Full Text Available to Nitrogen fixation protein nifU (SP:Q43885) {Anabaena azollae}; contains Pfam profile: PF01106 NifU-like domain 1e-74 ... ...AK060074 006-306-B05 At5g49940.1 nitrogen fixation NifU-like family protein similar

  9. Arabidopsis CDS blastp result: AK067601 [KOME

    Lifescience Database Archive (English)

    Full Text Available to Nitrogen fixation protein nifU (SP:Q43885) {Anabaena azollae}; contains Pfam profile: PF01106 NifU-like domain 1e-74 ... ...AK067601 J013108O03 At5g49940.1 nitrogen fixation NifU-like family protein similar

  10. Arabidopsis CDS blastp result: AK071874 [KOME

    Lifescience Database Archive (English)

    Full Text Available to Nitrogen fixation protein nifU (SP:Q43885) {Anabaena azollae}; contains Pfam profile: PF01106 NifU-like domain 3e-52 ... ...AK071874 J023121E22 At5g49940.1 nitrogen fixation NifU-like family protein similar

  11. Lista de cigarritas (Hemiptera: Cicadellidae de Cusco, Perú

    Directory of Open Access Journals (Sweden)

    Juan F. Costa

    2011-05-01

    Full Text Available Se presenta una lista de cigarritas registradas para Cusco, conteniendo 111 géneros y 203 especies. Esta lista incluye especies citadas en la literatura y también de material depositado en la colección de la Universidad Nacional de San Antonio Abad del Cusco. Las cigarritas identificadas por los autores fueron colectadas de 8 provincias de Cusco: Anta, Calca, Canchis, Cusco, La Convención, Paucartambo, Quispicanchi y Urubamba.

  12. Primer Registro para el Perú de Brachycaudus schwartzi (Bórner (Hemiptera: Aphididae

    Directory of Open Access Journals (Sweden)

    Walter Díaz

    2013-04-01

    Full Text Available Se registra por primera vez para el Perú la especie Brachycaudus schwartzi (Bórner (Hemiptera: Aphididae, “pulgón pardo del duraznero”. La especie ha sido colectada en el departamento de Tacna. Material de referencia se encuentra depositado en la Colección Referencial de Insectos de la Unidad del Centro de Diagnóstico de Sanidad Vegetal del SENASA, Lima, Perú.

  13. Sagitta chilensis nueva especie de quetognato en fiordos australes chilenos (Chaetognatha, Aphragmophora, Sagittidae) Sagitta chilensis a new species of chaetognath in Chilean fjords (Chaetognatha, Aphragmophora, Sagittidae)

    OpenAIRE

    Francisco Villenas; Sergio Palma

    2006-01-01

    Se describe por primera vez una nueva especie de quetognato del orden Aphragmophora, Sagitta chilensis n. sp., colectada en fiordos australes chilenos. Se analizaron 1065 ejemplares colectados entre la boca del Guafo y el canal Pulluche en noviembre de 2002. Estos ejemplares presentaron marcadas diferencias morfológicas con otras especies afines, como estructuras puntiagudas en el collarete y aletas, forma de aletas anteriores y posteriores, posición de las vesículas seminales y presencia de ...

  14. Parasitismo sobre eurysacca melanocampta meyrick (lepidoptera: gelechiidae) en dos localidades de cusco, perú.

    OpenAIRE

    Juan F. Costa; Yábar,Erick; Gianoli, Ernesto

    2011-01-01

    El cultivo de quinua (Chenopodium quinoa) es una importante actividad económica en Cusco. La polilla Eurysacca melanocampta (Lepidoptera: Gelechiidae) es la principal plaga registrada en este cultivo y presenta varios controladores biológicos. Se registran parasitoides y porcentajes de larvas parasitadas de la polilla de la quinua provenientes de dos localidades de Cusco: Izcuchaca (3400 msnm) y Quiquijana (3100 msnm). Las larvas colectadas se criaron en laboratorio hasta la emergencia de ...

  15. PARASITISMO SOBRE Eurysacca melanocampta Meyrick (LEPIDOPTERA: GELECHIIDAE) EN DOS LOCALIDADES DE CUSCO, PERÚ PARASITISM ON Eurysacca melanocampta Meyrick (LEPIDOPTERA: GELECHIIDAE) IN TWO LOCALITIES AT CUSCO, PERÚ

    OpenAIRE

    Juan F. Costa; Erick Yábar; Ernesto Gianoli

    2009-01-01

    El cultivo de quinua (Chenopodium quinoa) es una importante actividad económica en Cusco. La polilla Eurysacca melanocampta (Lepidoptera: Gelechiidae) es la principal plaga registrada en este cultivo y presenta varios controladores biológicos. Se registran parasitoides y porcentajes de larvas parasitadas de la polilla de la quinua provenientes de dos localidades de Cusco: Izcuchaca (3400 msnm) y Quiquijana (3100 msnm). Las larvas colectadas se criaron en laboratorio hasta la emergencia de los...

  16. Infestación por esparganos en ranas del genero Pristimantis (Anura, Strabomantidae del Perú

    Directory of Open Access Journals (Sweden)

    Luis A. Gomez-Puerta

    2011-05-01

    Full Text Available En el presente estudio, reportamos el parasitismo por esparganos del cestode Spirometra sp. en los anfibios: Pristimantis nephophilus y Pristimantis rhodostichus, provenientes del departamento de San Martin, Perú. Tres estructuras filiformes localizadas a nivel subcutáneo, fueron colectadas e identificadas como esparganos. El hallazgo de este metacestode constituye el primer registro en anfibios del Perú y demuestra que las ranas P. nephophilus y P. rhodostichus son nuevos hospederos intermediarios para Spirometra sp.

  17. Determinación experimental de las condiciones de operación para el proceso de hidrólisis enzimática de almidón de yuca nativa de la región amazónica en la ciudad de Leticia

    OpenAIRE

    Villada Pinilla, Wilher Andrés

    2010-01-01

    La amazonia colombiana cuenta con una amplia oferta de variedades de yuca que representan posibles alternativas industriales para la producción de azúcares fermentables y a partir de ellos obtener productos de mayor valor agregado como por ejemplo el alcohol biocombustible. En este trabajo, fueron identificadas y colectadas 21 variedades cultivadas por la asociación Agrovarzea1 en el plano inundable del río Amazonas (Leticia), las cuales fueron caracterizadas, priorizando tres de estas varied...

  18. CYANOBACTERIA FOR MITIGATING METHANE EMISSION FROM SUBMERGED PADDY FIELDS

    Energy Technology Data Exchange (ETDEWEB)

    Upasana Mishra; Shalini Anand [Department of Environmental Studies, Inderprastha Engineering College, Sahibabad, Ghaziabad (India)

    2008-09-30

    Atmospheric methane, a potent greenhouse gas with high absorption potential for infrared radiation, is responsible for one forth of the total anticipated warming. It is forming a major part of green house gases, next after carbon dioxide. Its concentration has been increasing alarmingly on an average at the rate of one percent per year. Atmospheric methane, originating mainly from biogenic sources such as paddy fields, natural wetlands and landfills, accounts for 15-20% of the world's total anthropogenic methane emission. With intensification of rice cultivation in coming future, methane emissions from paddy fields are anticipated to increase. India's share in world's rice production is next after to China and likewise total methane emission from paddy fields also. Methane oxidation through planktophytes, particularly microalgae which are autotrophic and abundant in rice rhizospheres, hold promise in controlling methane emission from submerged paddy fields. The present study is focused on the role of nitrogen fixing, heterocystous cyanobacteria and Azolla (a water fern harboring a cyanobacterium Anabaena azollae) as biological sink for headspace concentration of methane in flooded soils. In this laboratory study, soil samples containing five potent nitrogen fixer cyanobacterial strains from paddy fields, were examined for their methane reducing potential. Soil sample without cyanobacterial strain was tested and taken as control. Anabaena sp. was found most effective in inhibiting methane concentration by 5-6 folds over the control. Moist soil cores treated with chemical nitrogen, urea, in combination with cyanobacteria mixture, Azolla microphylla or cyanobacteria mixture plus Azolla microphylla exhibited significance reduction in the headspace concentration of methane than the soil cores treated with urea alone. Contrary to other reports, this study also demonstrates that methane oxidation in soil core samples from paddy fields was stimulated by

  19. 满江红孢子果空间诱变效应的研究--Ⅰ.高空条件对不同品系满江红孢子果生长发育的影响%A Study on the Effects of Space Induction of Sporocarps of Azolla——Ⅰ.Effect of space condition on the growth and development of different Azolla strains

    Institute of Scientific and Technical Information of China (English)

    郭文杰; 鲁雪华; 林勇; 陆培基; 刘中柱

    2002-01-01

    研究了高空条件对不同品系满江红孢子果诱变的效应,结果表明:高空处理对不同品系满江红孢子果的萌发率有明显的影响,但未出现遗传变异的突变体.经生育期观察,高空处理的19号满江红品系生长速率明显提高,且表现出一定的耐荫性.可望利用高空诱变作为满江红品种改良的有效手段.

  20. Comparative analysis of marine paleogene sections and biota from West Siberia and the Arctic Region

    Science.gov (United States)

    Akhmet'ev, M. A.; Zaporozhets, N. I.; Iakovleva, A. I.; Aleksandrova, G. N.; Beniamovsky, V. N.; Oreshkina, T. V.; Gnibidenko, Z. N.; Dolya, Zh. A.

    2010-12-01

    The analysis of the main biospheric events that took place in West Siberia and the Arctic region during the Early Paleogene revealed the paleogeographic and paleobiogeographic unity of marine sedimentation basins and close biogeographic relations between their separate parts. Most biotic and abiotic events of the first half of the Paleogene in the Arctic region and West Siberia were synchronous, unidirectional, and interrelated. Shelf settings, sedimentation breaks, and microfaunal assemblages characteristic of these basins during the Paleogene are compared. The comparative analysis primarily concerned events of the Paleocene-Eocene thermal maximum (PETM) and beds with Azolla (aquatic fern). The formation of the Eocene Azolla Beds in the Arctic region and West Siberia was asynchronous, although it proceeded in line with a common scenario related to the development of a system of estuarine-type currents in a sea basin partly isolated from the World Ocean.

  1. Freshwater aquatic plant biomass production in Florida

    Energy Technology Data Exchange (ETDEWEB)

    Reddy, K.R.; Sutton, D.L.; Bowes, G.

    1983-01-01

    About 8% (1.2 million ha) of the total surface area of Florida is occupied by freshwater. Many of these water bodies are eutrophic. Nutrients present in these water bodies can be potentially used to culture aquatic plants as a possible feedstock for methane production. This paper summarizes the results of known research findings on biomass production potential of freshwater aquatic plants in Florida and identifies key research needs to improve the quality and quantity of biomass yields. Among floating aquatic plants, biomass yield potential was in the order of water-hyacinth > water lettuce > pennywort > salvinia > duckweed > azolla. Pennywort, duckweed, and azolla appear to perform well during the cooler months compared to other aquatic plants. Among emergent plants, biomass yield potential was in the order of southern wild rice > cattails > soft rush > bulrush. Cultural techniques, nutrient management, and environmental factors influencing the biomass yields were discussed. 68 references.

  2. Evaluation of butachlor for control of some submerged macrophytes along with its impact on biotic components of freshwater system

    Directory of Open Access Journals (Sweden)

    A. Chattopadhyay, S. Adhikari, S. P. Adhikary, S. Ayyappan

    2006-04-01

    Full Text Available In this investigation, the efficacy of the herbicide butachlor, (N-butoxymethyl-2 chloro-21, 61 diethyl acetanilide was tested against few common submerged macrophytes namely Hydrilla (Hydrilla verticillata (L. Royale, Najas (Najas minor All., Nechamandra (Nechamandra alternifolia (Roxb. Thwaites and Ottelia (Ottelia alismoides (L. Pers. of freshwater fish ponds. Almost complete decay of Hydrilla, Nechamandra and Ottelia was achieved at 7.5 L of active ingredient/ha/m butachlor within 15 days while the herbicide showed no negative effect on Najas. However at the same concentration of butachlor, total mortality of zooplankton and water fern Azolla (Azolla caroliniana Lamarck occurred within seven days. In case of few freshwater fish species like Rohu (Labeo rohita, Channa (Channa punctatus, Anabas (Anabas testitudineus and Heteropneustes (Heteropneustes fossilis, total mortality occurred upto 90 days after application of the same dose of butachlor but fish survived beyond 120 days of herbicide application indicating degradation of the herbicides.

  3. Photoproduction of molecular hydrogen by a plant-algal symbiotic system

    Energy Technology Data Exchange (ETDEWEB)

    Newton, J.W.

    1976-02-13

    The rapidly growing water fern Azolla, which contains a nitrogen-fixing blue-green algal symbiont, has been studied as a possible system for photoproduction of molecular hydrogen. When this plant is grown on a combined nitrogen supply, photochemically generated hydrogen can be diverted through the algal nitrogenase system, which serves as a source of molecular hydrogen generated from water. This symbiosis has several advantages as a possible biological energy conversion system. (auth)

  4. Macrophytes of Lake Victoria and succession after invasion of Water Hyacinth

    OpenAIRE

    Omondi, R.; Kusewa, Mwende

    2006-01-01

    The distribution of Lake Victoria (Kenya) macrophytes is described. Succession of macrophytes in the lake became more dramatic and dynamic after invasion of water hyacinth. The weed pushes and smothers other free-floating macrophytes like Pistia stratiotes. It then provides substrates for the emergent Vossia cuspidata, which later reduces its population by competition for light and nutrients. The noxious weed is believed to have led to extinction of Azolla nilotica in the lake.

  5. Phylogeny, diversity and toxin production related to cyanobacterial symbioses

    OpenAIRE

    Papaefthimiou, Dimitra

    2007-01-01

    Phylogeny and morphology were examined for the cyanobionts from the water fern Azolla and the cyanobacterial genus Nostoc originating from symbioses with different host plants (genera Gunnera, Cycas, Dioon, Encephalarthos, Macrozamia, and Anthoceros), the lichen genus Pannaria, and free-living Nostoc isolates from different habitats. Nostoc isolates of Pannaria formed a closely related group, but, in general, no monophyletic nature was attributed to the genus Nostoc, in contrast to the cyanob...

  6. 河南外来入侵植物新报%New materials to the alien invasive plants in Henan

    Institute of Scientific and Technical Information of China (English)

    张云霞; 刘兆云; 陈付合; 朱长山

    2010-01-01

    报道新确认的河南外来入侵植物9种:细叶满江红(Azolla filiculioides)、野老鹳草(Geranium carolinianum)、假酸浆(Nicandra physaloides)、毛酸浆(Physalis pubescens)、牛茄子(Solanum surattense)、北美车前(Plantago virginica)、霍香蓟(Ageratum conyzoides)、鬼针草(Bidens pilosa)和两耳草(Paspalum conjugatum).

  7. 千葉大学園芸学部松戸キャンパスにおけるシダ植物フロラと生育状況

    OpenAIRE

    水野,大樹; Taiki Mizuno; 竹﨑, 大悟; Hirosato Takezaki; 安田,正次; Masatsugu Yasuda; 百原,新; Arata Momohara; 沖津, 進; Susumu Okitsu

    2011-01-01

    Fern flora in the Matsudo campus has not been studied since 1933. To clarify existing species diversity of fernwhich is susceptible to urbanization and disturbance by planting, we made up a fern flora of the Matsudo Campus,Faculty of Horticulture, Chiba University. We found 34 taxa of ferns including 12 family, 17 genus, 31 species, 1subspecies, 1 variety, and 1 hybrid species. Thirty species including aquatic ferns such as Azolla imbricata,Salvinia natans, and Marsilea quadrifolia have disap...

  8. Heavy metal pollution in lentic ecosystem of sub-tropical industrial region and its phytoremediation.

    Science.gov (United States)

    Rai, Prabhat Kumar

    2010-03-01

    Metals and several physicochemical parameters, from four sampling sites in a tropical lake receiving the discharges from a thermal power plant, a coal mine and a chlor-alkali industry, were studied from 2004-2005. Pertaining to metal pollution, the site most polluted with heavy metals was Belwadah, i.e., waters and sediments had the highest concentration of all the metals examined. The reference site was characterized by the presence of low concentrations of metals in waters and in sediments. Further, several wetland plants were harvested from different sites, and simultaneously, these were assessed for their metal concentration efficiency. Following the water quality monitoring and metal concentration efficiency, two-month field phytoremediation experiments were conducted using large enclosures at the discharge point of different polluted sites of the lake. Eichhornia crassipes, Lemna minor, and Azolla pinnata were frontier metal accumulators hence selected for previously mentioned field phytoremediation experiments. During field phytoremediation experiments using aquatic macrophytes, marked percentage reduction in metals concentrations were recorded. The percentage decrease for different metals was in the range of 25-67.90% at Belwadah (with Eichhornia crassipes and Lemna minor), 25-77.14% at Dongia nala (with Eichhornia crassipes, Lemna minor, and Azolla pinnata) and 25-71.42% at Ash pond site of G.B. Pant Sagar (with Lemna minor and Azolla pinnata). Preliminary studies of polluted sites are therefore useful for improved microcosm design and for the systematic extrapolation of information from experimental ecosystems to natural ecosystems. PMID:20734618

  9. Fate of N from Green Manures and Ammonium Sulfate

    Institute of Scientific and Technical Information of China (English)

    SHISHU-LIAN; WENQI-XIAO; 等

    1991-01-01

    By means of 15N tracer technique the fate of N in ammonium sulfate,milk vetch,sesbania and azolla,and the availability of their residual N were studied in a microplot experiment.Results showed that a) at the end of the first crop of early rice,both plant recovery and loss of N from ammonium sulfate were the highest whereas those from azolla were the lowest with those from milk vetch and sesbania in between;the sequence was reversed in terms of recovery of N in soil;the net residual N from ammonium sulfate was very low,about 1/7-1/4 of that from green manures,indicating that chemical N fertilizer contributes little to the soil N reserve;b) plant recovery of the residual N was low and it did not always decrease with time;the total plant recovery (from the second to the fifth crops) of the residual N from various test fertilizers was only 8-11% of the total N originally applied;c) the plant recovery of the residual N from ammonium sulfate was the highest,followed by those from milk vetch and sesbania,and that from azolla was the lowest,no matter in which cropping season (from the second to the fifth);N availability ratio showed the same trend,indicating that chemical N fertilizer helps renovate soil organic N,maintain and increase availability of soil N.

  10. IgG antibodies to dengue in dry blood samples in filter paper.

    Directory of Open Access Journals (Sweden)

    Carmen Acosta

    2003-01-01

    Full Text Available En este trabajo se presentan los resultados de la normalización del procedimiento de detección de anticuerpos IgG contra el virus dengue en muestras de sangre colectadas sobre papel de filtro. Las muestras se obtuvieron de 216 individuos que acudieron al banco de sangre de Marianao de los cuales 25 presentaron resultados con títulos superiores a 1:40 por la técnica de Inhibición de la Hemaglutinación. En todas las muestras se determinó la presencia de anticuerpos IgG contra el virus dengue mediante un inmunoensayo enzimático (UMELISA de captura. Cuando se compararon las condiciones de elución de treinta minutos y una hora la coincidencia fue del 93 y 100 % respectivamente. En las dos condiciones evaluadas en el paso de la incubación de las muestras en la placa de reacción; una hora a 37 ºC y una hora a temperatura ambiente; la coincidencia fue del 100 %, manteniendo este paso igual para el procesamiento de muestras de sangre colectadas sobre papel de filtro como las de suero (una hora a 37 ºC. Al analizarse en su conjunto los resultados de las muestras se obtuvo una sensibilidad y especificidad del 100 % en los tiempos ensayados, para la prueba efectuada con sangre total colectada sobre papel de filtro conservada a 4 ºC y las conservadas a temperatura ambiente. Los autores recomiendan la utilización de este método para la detección de anticuerpos anti dengue.

  11. Contenido en fenoles totales y actividad antioxidante de extractos de flores de Achyrocline satureioides provenientes de diferentes zonas de Argentina

    OpenAIRE

    Ferraro, Graciela Ester; Anesini, Claudia; Ouviña, Adriana; Retta, Daiana; Filip, Rosana; Gattuso, Martha; Gattuso, Susana; Hnatyszyn, Oksana; Bandoni, Arnaldo L.

    2008-01-01

    Achyrocline satureioides es ampliamente empleada, a lo largo de Argentina, Brasil y Uruguay, en medicina popular. En este estudio, hemos comparado el contenido de fenoles totales, expresados como equivalente/g de ácido gálico (mg de GAE/g de muestra), utilizando extractos de flores de A. satureioides, colectadas en diferentes zonas de Argentina. Los extractos fueron además evaluados por su actividad antioxidante, monitoreando la velocidad de decoloración del 1,1-difenil-2-picrilhidrazil (DDPH...

  12. Evaluación fitoquímica preliminar de tres especies cubanas de Erythroxylum

    OpenAIRE

    Cazaña-Martínez, Yanet; González Lavaut, José Antonio; Prieto-González, Sylvia; Molina Torres Jorge; González-Guevara, Johannes; Urquiola Cruz, Armando

    2004-01-01

    Se realizó la evaluación fitoquímica preliminar de hojas de Erythroxylum areolatum L., E. suave Lunan y E. havanense, Jacq., colectadas en diferentes épocas en varias localidades de la provincia de Pinar del Río, Cuba. Se encontró correspondencia de la composición de metabolitos presentes (alcaloides, flavonoides, fenoles-taninos, triterpenos- esteroides) entre las especies evaluadas y los reportes bibliográficos para el género, tanto para el material vegetal crudo como para los extractos acu...

  13. Primeros registros de helmintos parásitos en Fulica ardesiaca (Aves: Rallidae) para el Perú : Pantanos de Villa – Lima

    OpenAIRE

    Gisella Guillén; Elizabeth Morales

    2013-01-01

    En el presente trabajo se registran por primera vez para el Perú, los parásitos de un ejemplar de Fulica ardesiaca «gallareta andina» colectada en los Pantanos de Villa. Se colectaron un total de 698 especimenes: los tremátodos Notocotylus pacifera y Psilostomum sp. con 529 y 149 individuos respectivamente y el céstodo Diorchis americana, con 20. El presente trabajo constituye el primer reporte para el Perú de helmintos en F. ardesiaca y Psilostomum sp. y D. americana son primeros registros p...

  14. Foraminíferos planctónicos vivos en el mar ecuatoriano: Taxonomía y distribución.

    OpenAIRE

    Luzuriaga de Cruz, María

    2014-01-01

    La sensibilidad a las variaciones del ambiente marino por parte de las especies planctónicas como los foraminíferos planctónicos, convierten a estos organismos en herramientas vivas de detección de cambios del ecosistema marino en momento dado. El presente trabajo trata principalmente sobre la taxonomía y distribución de especies de foraminíferos planctónicos identificados en el mar ecuatoriano desde 1973 a 1998. Las muestras de zooplancton fueron colectadas a bordo de cruceros oceanográficos...

  15. Actividad molusquicida del Piquerol A aislado de Piqueria trinervia (compositae) sobre ocho especies de caracoles pulmonados The molluscicide activity of Piquerol A isolated from Piqueria trinervia (Compositae) against eight species of pulmonate snails

    OpenAIRE

    Alejandro Cruz-Reys; Carolina Chavarin; Martha P. Campos Arias; Javier Taboada; Manuel Jímenez E.

    1989-01-01

    De las partes aéreas de la planta Piqueria trinervia (Compositae) colectada en diveresas áereas de México, se aisló el Piquerol A. Este compuesto se probó como agente molusquicida contra ocho especies de caracoles pulmonados: Fossaria (Fossaria) humilis, F. (Bakerilymnae) sp., Pseudosuccinea columella, Stagnicola attenuata, de México; F. (B.) cubensis y Physacubensis, de Cuba; P. Columella y Biomphalaria glabrata, de Brasil; B glabrata, de Puerto Rico; S. elodes, de Estados Unidos. Se utiliza...

  16. Residuos de plaguicidas organoclorados en leche pasteurizada comercializada en Ciudad de México Organochlorine pesticide residues in commercial pasteurised milk in Mexico City

    OpenAIRE

    Prado, G.; Diaz, G; S. VEGA Y LEON; Gonzalez, M; Perez, N.; Urban, G.; Gutierrez, R.; Ramirez, A.; Pinto, M.

    1998-01-01

    Los residuos de algunos plaguicidas organo-clorados presentes en lasmuestras de leche pasteurizada analizadas representan un riesgo para lasalud de los consumidores. Se determinó el contenido de plaguicidas organoclorados en 96 muestras de leche pasteurizada, por cromatografía gas líquido. Las muestras fueron colectadas quincenalmente y durante un año en la Ciudad de México, de cuatro marcas comerciales.En las muestras analizadas se determinó el valor promedio X en µg/gbase grasa, la razón X/...

  17. Hábitos alimentarios del Coyote en el parque nacional Pico de Orizaba

    OpenAIRE

    Jesús Martínez-Vázquez; Rosa María González-Monroy; Diana Díaz-Díaz

    2010-01-01

    Se examinó la dieta del coyote (Canis latrans) en el Parque Nacional Pico de Orizaba mediante el análisis de 136 heces fecales que fueron colectadas mensualmente por 11 meses en un transecto de 7 km. Los resultados indican que la dieta de coyote se compone principalmente de cuatro grupos tales como: mamíferos (48.96%), plantas (20.28%), aves (6.76%) e insectos (0.68%). La dieta anual del coyote estuvo conformada por 15 especies-presa. Los porcentajes más altos de la dieta están constituidos p...

  18. RENDIMIENTO DE GRANO Y SUS COMPONENTES EN MAÍCES NATIVOS DE TAMAULIPAS EVALUADOS EN AMBIENTES CONTRASTANTES

    OpenAIRE

    J. Agapito Pecina Martínez; Ma. del Carmen Mendoza Castillo; J. Alberto López Santillán; Fernando Castillo González; Moisés Mendoza Rodríguez; Joaquín Ortiz Cereceres

    2011-01-01

    Entre las poblaciones nativas de maíz (Zea mays L.) del Estado de Tamaulipas, México, algunas destacan por su alto potencial de rendimiento de grano, pero se han aprovechado en grado limitado. En el presente trabajo se evaluó el rendimiento y sus componentes en 29 poblaciones nativas de maíz de Tamaulipas (colectadas durante los años 2001 al 2004), más seis variedades mejoradas, en tres ambientes contrastantes en altitud y temperatura. El objetivo fue identificar poblaciones sobresalientes qu...

  19. CARACTERIZACIÓN NUTRICIONAL DEL GRANO DE 50 ACCESIONES DE MAÍZ CUBANO

    OpenAIRE

    Martínez, M.; Natalia Palacios; Ortiz, R.

    2009-01-01

    En este trabajo se realizó un análisis de la calidad nutricional a 50 accesiones de maíz amarillo, colectadas en fincas de agricultores de las tres regiones de Cuba (occidente, centro y oriente). Para ello, se examinaron los caracteres siguientes: contenidos (%) de ceniza, aceite, azúcares totales, nitrógeno, lisina y triptófano, en el Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT) en México. Como resultado se obtuvieron valores normales de ceniza, aceite, azúcares y lisina; si...

  20. Balances de nutrientes en pequeñas cuencas de encinar. II: quimismo de la precipitación y aportes de origen atmosférico

    OpenAIRE

    Bellot Abad, Juan Francisco; Escarré Esteve, Antonio

    1988-01-01

    Durante un período de tres años, se ha estudiado el quimismo del agua de lluvia y se han estimado los aportes de nutrientes en la deposición global a una cuenca de encinar en la serra de Prades (Tarragona, España). En ninguno de los iones analizados se ha observado diferencia significativa entre las muestras colectadas en dos altitudes distintas: 700 y 950 m. s. n. m. La precipitación es moderadamente acida (pH = 4.96) y los iones más importantes en la lluvia son SO24- y Ca2+, ...

  1. Primeros registros de helmintos parásitos en Fulica ardesiaca (Aves: Rallidae para el Perú : Pantanos de Villa – Lima

    Directory of Open Access Journals (Sweden)

    Gisella Guillén

    2013-06-01

    Full Text Available En el presente trabajo se registran por primera vez para el Perú, los parásitos de un ejemplar de Fulica ardesiaca «gallareta andina» colectada en los Pantanos de Villa. Se colectaron un total de 698 especimenes: los tremátodos Notocotylus pacifera y Psilostomum sp. con 529 y 149 individuos respectivamente y el céstodo Diorchis americana, con 20. El presente trabajo constituye el primer reporte para el Perú de helmintos en F. ardesiaca y Psilostomum sp. y D. americana son primeros registros para la helmintofauna peruana.

  2. Caracterización bioquímica y fisiológica de algunos frutos amazónicos (Capsicum sp. Y Eugenia stipitata MC VAUGH)

    OpenAIRE

    Hernández María Soledad; Melgarejo Luz Marina; Manduca Fermín Juan Francisco

    2006-01-01

    Los niveles de la actividad poligalacturonasa (PG) fueron cuantificados en diferentes estadios de madurez de frutos de cuatro especies de ají Capsicum chinense, Capsicum baccatum, Capsicum annuum y Capsicum frutescens. Las muestras fueron colectadas y almacenadas a -10º C. La determinación de la actividad poligalacturonasa fue medida por el método de azúcares reductores de Somogyi-Nelson y el contenido de proteínas por Bradford. El comportamiento de la actividad PG de la especie C. chinense e...

  3. Estudio de las macroalgas marinas del puerto de Salina Cruz, Oaxaca: redescubrimiento de Predaea subpeltata y nuevo registro de Cryptonemia angustata para el golfo...

    OpenAIRE

    Jorge Galindo Villegas; José A. Gamboa; Kurt M. Dreckmann

    1997-01-01

    Veintiocho especies de macroalgas marinas fueron identificadas viviendo en el rompeolas del puerto de Salina Cruz en el Golfo de Tehuantepec, Pacífico tropical mexicano. Se discute la identidad de Predaea subpeltata E. Y. Dawson (Gymnophoeaceae, Rhodophyta), especie que no había sido colectada desde su descripción original para el Puerto de Huatulco, Oaxaca hace 25 años. Asimismo, una colección de algas cistocárpicas asignadas a Cryptonemia angustata (Setchell Gardner) E.Y. Dawson (Halymeni...

  4. Estudio fitoquímico de la corteza de acacia farnesianautilizada tradicionalmente para el tratamiento de la malaria

    OpenAIRE

    Daza Bareño, Ada Zurima

    2014-01-01

    En Colombia se ha reportado el uso de decocciones de las hojas y la corteza de Acacia farnesiana para el tratamiento de fiebres y malaria. El presente trabajo es una contribución al estudio fitoquímico de la corteza de esta especie colectada en dos épocas del año, época seca (junio) y de lluvia (octubre). Para tal fin se recolectó el material vegetal en Armero-Guayabal, Departamento Tolima (Colombia) y se realizó una caracterización fitoquímica del extracto etanólico obtenido por percolación ...

  5. COMBARBALA-INIA, UN CULTIVAR PRECOZ DE HUALPUTRA (Medicago polymorpha L.) PARA AREAS DE SECANO MEDITERRANEO. Combarbalá-INIA, an early flowering cultivar of burr medic (Medicago polymorpha L.) for Mediterranean dryland areas.

    OpenAIRE

    Alejandro del Pozo L.; Carlos Ovalle M; Julia Avendaño R.; Teresa Aravena; M. Elena Díaz

    2001-01-01

    Combarbalá-INIA es un nuevo cultivar de hualputra (Medicago polymorpha L.), seleccionado entre un gran número de accesiones colectadas en Chile, entre La Serena (29º 55’ lat. Sur) y Temuco (38º 47’ lat. Sur). Es una leguminosa forrajera anual, precoz (71 - 83 días desde emergencia a primera flor), apta para áreas de secano de la zona mediterránea árida, semiárida y subhúmeda, es decir, entre la IV y VI Regiones de Chile. Presenta un hábito de crecimiento postrado, flores de color amarillo, y ...

  6. CARACTERIZACIÓN DE LA DIVERSIDAD Y SELECCIÓN PARTICIPATIVA DE PROSPECCIONES DE MAÍZ EN CHIAPAS, MÉXICO

    Directory of Open Access Journals (Sweden)

    M. Martínez

    2006-01-01

    Full Text Available En marzo y abril del 2003, se realizó una prospección de maíz en 20 municipios pertenecientes a cinco regiones del estado de Chiapas, México. Se lograron colectar 331 accesiones de maíz, de las cuales dos son donadas por el INIFAP y el resto son colectadas en las fincas de los campesinos; dentro de estas accesiones hay granos de color negro, blanco, amarillo, rojo y pinto. El 30 de mayo del 2003, en el Centro de Desarrollo Comunitario CEDECO ¿La Albarrada¿, ubicado en el municipio San Cristóbal de las Casas, se realizó la siembra de 85 accesiones de maíz, colectadas en localidades que se encontraban en un rango de altitud entre 1 500 y 2 260 m snm. Durante el ciclo del cultivo se realizaron diferentes evaluaciones, mostrándose una amplia variabilidad tanto entre las accesiones de diferentes colores como dentro de ellas; además, la variabilidad también se muestra entre las diferentes localidades donde fueron colectadas. El 6 de diciembre del 2003, se realizó una feria de biodiversidad a la que asistieron campesinos provenientes de cinco municipios del estado de Chiapas. Durante la feria de las 85 accesiones expuestas, los campesinos seleccionaron al menos una vez 51, lo que constituye el 60 % de toda la diversidad expuesta. Los campesinos mostraron un mayor interés por las accesiones de granos de color negro y amarillo, constituyendo el 66.6 % de la diversidad colectada. Durante la selección, los hombres y las mujeres tuvieron diferentes criterios a la hora de seleccionar las accesiones y, además, difirieron en el número de accesiones seleccionadas. En general, se mostró un gran interés por parte de los campesinos en accesiones criollas que mostraran cierta tolerancia a las condiciones específicas de sus fincas.

  7. PROMOCIÓN DEL CRECIMIENTO DE Baccharis macrantha (ASTERACEAE) CON BACTERIAS SOLUBILIZADORAS DE FOSFATOS ASOCIADAS A SU RIZOSFERA

    OpenAIRE

    Luz Marina LIZARAZO FORERO; Elsa Giovanna ÁVILA MARTÍNEZ; Francisco CORTÉS PÉREZ

    2015-01-01

    El objetivo de esta investigación fue aislar y caracterizar bacterias solubilizadoras de fosfatos (BSF) asociadas a la rizosfera de Baccharis macrantha y Viburnum triphyllum, y evaluar su capacidad para solubilizar fosfatos en condiciones in vitro. Además se determinó el efecto de la inoculaciónde las cepas de BSF más eficientes sobre el crecimiento de B. macrantha. Las muestras de suelo rizosférico de B. macrantha y V. triphyllum fueron colectadas en los meses de mayo-período de lluvia y sep...

  8. IgG antibodies to dengue in dry blood samples in filter paper.

    OpenAIRE

    Carmen Acosta; Regla Herrera; Pedro Almenarez; Susana Vázquez

    2003-01-01

    En este trabajo se presentan los resultados de la normalización del procedimiento de detección de anticuerpos IgG contra el virus dengue en muestras de sangre colectadas sobre papel de filtro. Las muestras se obtuvieron de 216 individuos que acudieron al banco de sangre de Marianao de los cuales 25 presentaron resultados con títulos superiores a 1:40 por la técnica de Inhibición de la Hemaglutinación. En todas las muestras se determinó la presencia de anticuerpos IgG contra el virus dengue me...

  9. Evaluación de la composición química (Filantósidos y Lignanos) de los extractos crudos del árbol de Chilillo (Phyllantus Acuminatus Vahl) procedente de diferentes sitios de Costa Rica

    OpenAIRE

    Daniel Esquivel Alvarado; Gerardo Rodríguez Rodríguez; Sergio Madrigal Carballo

    2013-01-01

    El propósito del presente trabajo fue en primera instancia el realizar un estudio del contenido de filantósidos y lignanos presentes en muestras de diferentes partes del árbol de chilillo (Phyllantus acuminatus Vahl), colectadas en diferentes sitios del país. Para la primera parte del estudio se utilizó cromatografía de alta eficiencia (HPLC). Los resultados de estas pruebas mostraron no solo variación en el número de picos del cromatograma, sino también variaciones en la intensidad, en funci...

  10. Efecto in vitro de aceites esenciales de tres especies de lippia sobre moniliophthora roreri (cif. y par.) evans et al., agente causante de la moniliasis del cacao (theobroma cacao l.)

    OpenAIRE

    Lozada, Betty Stefany; Herrera, Laura Viviana; Perea, Janeth Aidé; Stashenko, Elena; Escobar, Patricia

    2012-01-01

    Se evaluó el efecto antifúngico in vitro de cinco aceites esenciales (AEs) (AE1, AE2, AE3, AE4 y AE5) extraídos de Lippia origanoides, L. citriodora y L. alba sobre aislados de monilia (Moniliophthora roreri) obtenidos de frutos de cacao infectados provenientes de San Vicente de Chucurí, Santander, Colombia. Las plantas de Lippia fueron colectadas en cinco localidades colombianas. Los aislados de monilia (M1, M2, M3, M4 y M5) fueron caracterizados por su morfología, germinación y crecimiento ...

  11. Efecto in vitro de aceites esenciales de tres especies de Lippia sobre Moniliophthora roreri (Cif. y Par.) Evans et al., agente causante de la moniliasis del cacao (Theobroma cacao L.) In vitro effect of essential oils of three Lippia species on Moniliophthora roreri (Cif. and Par.) Evans et al., causative agent of moniliasis of cocoa (Theobroma cacao L.)

    OpenAIRE

    Betty Stefany Lozada; Laura Viviana Herrera; Janeth Aidé Perea; Elena Stashenko; Patricia Escobar

    2012-01-01

    Se evaluó el efecto antifúngico in vitro de cinco aceites esenciales (AEs) (AE1, AE2, AE3, AE4 y AE5) extraídos de Lippia origanoides, L. citriodora y L. alba sobre aislados de monilia (Moniliophthora roreri) obtenidos de frutos de cacao infectados provenientes de San Vicente de Chucurí, Santander, Colombia. Las plantas de Lippia fueron colectadas en cinco localidades colombianas. Los aislados de monilia (M1, M2, M3, M4 y M5) fueron caracterizados por su morfología, germinación y crecimiento ...

  12. Efecto in vitro de aceites esenciales de tres especies de Lippia sobre Moniliophthora roreri (Cif. y Par.) Evans et al., agente causante de la moniliasis del cacao (Theobroma cacao L.)

    OpenAIRE

    Betty Stefany Lozada; Laura Viviana Herrera; Janeth Aidé Perea; Elena Stashenko; Patricia Escobar

    2012-01-01

    Se evaluó el efecto antifúngico in vitro de cinco aceites esenciales (AEs) (AE1, AE2, AE3, AE4 y AE5) extraídos de Lippia origanoides, L. citriodora y L. alba sobre aislados de monilia (Moniliophthora roreri) obtenidos de frutos de cacao infectados provenientes de San Vicente de Chucurí, Santander, Colombia. Las plantas de Lippia fueron colectadas en cinco localidades colombianas....

  13. Nuevos registros de clorofitas marinas para Cuba

    OpenAIRE

    A. Moreira; Fujii, M.T.; Suárez, A. M.; Wynne, M.J.

    2013-01-01

    A partir de muestras colectadas en la bahía de Cienfuegos y de Santa Lucía (Pinar del Río), se identificaron dos nuevos registros de macroalgas marinas (clorofitas) para Cuba: Cladophora blomquistii C. Hoek y Udotea fibrosa D. S. Littler & M. M. Littler. C. blomquistii se registra por primera vez para la región del Caribe ya que se había consignado solo para lagunas costeras de la Florida, y la parte noreste del golfo de México. Se ofrecen además comentarios sobre la taxonomía, ...

  14. Caracterización de Klebsiella pneumoniae productora de la beta-lactamasa SHV-5, en una unidad de cuidados intensivos Characterization of SHV-5 beta-lactamase-producing Klebsiella pneumoniae in an intensive care unit

    OpenAIRE

    Verónica Andrade; Jesús Silva

    2004-01-01

    OBJETIVO: Caracterizar molecularmente los aislamientos de Klebsiella pneumoniae obtenidos de pacientes pediátricos y del personal de salud en la unidad de cuidados intensivos de un hospital de tercer nivel de atención en la Ciudad de México, Distrito Federal. MATERIAL Y MÉTODOS: Se analizaron 15 aislamientos de Klebsiella pneumoniae colectadas de un brote durante el mes de junio de 1996, ocho de pacientes y siete de personal del Hospital Infantil de México. Los aislamientos fueron caracteriza...

  15. Caracterización de Klebsiella pneumoniae productora de la b-lactamasa SHV-5, en una unidad de cuidados intensivos Klebsiella pneumoniae

    OpenAIRE

    Verónica Andrade; Jesús Silva

    2004-01-01

    Objetivo. Caracterizar molecularmente los aislamientos de Klebsiella pneumoniae obtenidos de pacientes pediátricos y del personal de salud en la unidad de cuidados intensivos de un hospital de tercer nivel de atención en la Ciudad de México, Distrito Federal. Material y métodos. Se analizaron 15 aislamientos de Klebsiella pneumoniae colectadas de un brote durante el mes de junio de 1996, ocho de pacientes y siete de personal del Hospital Infantil de México. Los aislamientos fueron caracteriza...

  16. RESPUESTA DE LA POLILLA DEL TOMATE, Tuta absoluta (Meyrick), A INSECTICIDAS EN ARICA Tomato moth, Tuta absoluta (Meyrick) response to insecticides in Arica, Chile

    OpenAIRE

    Erika R. Salazar; Jaime E Araya

    2001-01-01

    Se comparó la susceptibilidad larvaria de la polilla del tomate, Tuta absoluta (Meyrick), colectada en tomate (Lycopersicon esculentun Mill.) en Azapa, Arica (18º 31’ lat. Sur, 70º 11’ long. Oeste), mediante pruebas de toxicología con varias dosis de insecticidas de uso común, aplicados sobre grupos de larvas de dos niveles de desarrollo (estadíos 1-2 y 3-4). Para determinar la resistencia a los insecticidas se calcularon las DL50, DL90 y pendientes de las regresiones entre mortalidad (probit...

  17. ISOLATION OF Ricket tsia bellii FROM Amblyomma ovale AND Amblyomma incisum TICKS FROM SOUTHERN BRAZIL

    OpenAIRE

    Richard Pacheco; Simone Rosa; Leonardo Richtzenhain; Szabó, Matias P. J.; Marcelo B Labruna

    2008-01-01

    Objetivo. Aislar Rickettsias mediante cultivo celular a partir de muestras de garrapatas Amblyomma ovale y Amblyomma incisum del estado de São Paulo Materiales y métodos. A. ovale y A. incisum adultas de vida libre fueron colectadas en una área de selva tropical Atlántica en el estado de São Paulo, Brazil. Cada garrapata fue sometida a la prueba de hemolinfa, las garrapatas positivas en esta prueba fueron evaluadas con la técnica de shell vial con el propósito de aislar rickettsias en cultivo...

  18. Detección de Rickettsia spp. en Garrapatas de Myrmecophaga tridactyla de Vida Libre en la Sabana Inundable de Casanare‚ Colombia -resumen-

    OpenAIRE

    César Rojano-Bolaño; R Ávila-Avilán; L Miranda-Cortés; V Contreras-Cogollo; Jorge Miranda-Regino; Laura Hernández-Fernández; Salim Mattar-Velilla; Santiago Monsalve-Buriticá; J Carrascal-Velásquez

    2014-01-01

    Las bacterias del género Rickettsia son organismos con distribución mundial‚ causantes de algunas enfermedades zoonóticas de gran importancia en salud pública. Sin embargo‚ en Colombia son pocos los estudios enfocados a conocer su distribución y los vectores involucrados. El objetivo de este estudio fue detectar la presencia de Rickettsia spp.‚ en garrapatas colectadas en nueve osos palmeros (Myrmecophaga tridactyla) de vida libre en el municipio de Pore‚ Casanare‚ entre los años 2013 y 2014....

  19. Caracterización fisiológica, físico-química, reológica, nutraceútica, estructural y sensorial de la guanábana (Annona muricata L. cv. Elita)

    OpenAIRE

    Márquez Cardozo, Carlos Julio

    2009-01-01

    Se utilizaron frutas de guanábana (Annona muricata L. cv. Elita) obtenidas de huertos establecidos en la zona agroindustrial del Valle del Cauca, (Colombia), sector rural del municipio de Pradera, Agrícola Varahonda, Finca la Española, localizada a 1.070 msnm. 23°C de temperatura promedio, 1.225 mm de precipitación promedio anual, radiación solar media de 4,8 W•m-2•día y humedad relativa promedio de 83%. Las frutas fueron colectadas en igual grado de madurez, correspondiente a 16 semanas lueg...

  20. Micromorfología de ceras epicuticulares en hojas maduras de Sabal yapa Wright ex Becc. (Arecaceae)

    OpenAIRE

    Martha Pérez-García; José David Sepúlveda-Sánchez

    2011-01-01

    Se reporta la presencia de cera epicuticular sobre la cutícula de la epidermis de las superficies adaxial y abaxial de la lámina de la hoja de Sabal yapa Wright ex Becc., observada con el microscopio electrónico de barrido (MEB). Las muestras foliares fueron colectadas en una vegetación secundaria de la selva mediana subperennifolia en Quintana Roo y en dos huertos de Yucatán. Se describió la micromorfología de la cera (siguiendo la clasificación de Barthlott et al., 1998); se observaron dos ...

  1. Pigmentación de Fusarium verticillioides (Sacc.) como factor de virulencia en plántulas de maíz en México.

    OpenAIRE

    Solano Báez, Alma Rosa

    2010-01-01

    Existen antecedentes de que los pigmentos producidos por especies de Fusarium tienen efecto fitotóxico en plantas hospedantes de este patógeno, son inhibidores de germinación de semillas, además de actividad antibacterial. La influencia que pudieran tener en la virulencia aun no se define, por lo que en esta investigación se obtuvieron mazorcas de maíz, asintomáticas y con síntomas de pudrición de mazorca que fueron colectadas en el ciclo primavera-verano de 2008 del estado de Veracruz Méxi...

  2. EVALUACIÓN PRELIMINAR DE LAS COMUNIDADES DE MACROINVERTEBRADOS BENTÓNICOS DE LA LAGUNA DE QUISTOCOCHA, IQUITOS, PERÚ

    Directory of Open Access Journals (Sweden)

    Jemima Alván Alvarado

    2012-12-01

    Full Text Available El objetivo del presente trabajo fue realizar una evaluación preliminar de la riqueza y abundancia de las comunidades de macroinvertebrados bentónicos colectados en dos estratos (orilla y 1 metro de profundidad de la Laguna de Quistococha (Loreto, Perú. Las muestras de orilla fueron colectadas utilizándose una red de colecta de bentos (en forma de D con malla de 1 mm2, mientras que las de 1 m de profundidad fueron colectadas con una draga tipo Eckman, colectándose en total 30 muestras (15 de orilla y 15 de 1 m de profundidad. En la orilla se registró la mayor riqueza (11 especies y abundancia (109 individuos de macroinvertebrados bentónicos, en comparación con la de 1 m de profundidad, donde solo se registraron 5 especies y 51 individuos, registrándose diferencias significativas según la prueba de Mann-Whitney (P<0,05. Para todas las zonas de colecta, la especie Chironomus sp. fue la más abundante tanto en orilla (79 individuos, 72,48%, como a 1 m de profundidad (43 individuos, 84,31%.

  3. Effects of organic matter application on methane emission from paddy fields adopting organic farming system

    Directory of Open Access Journals (Sweden)

    P Nungkat

    2015-01-01

    Full Text Available A study that was aimed to determine the effect of the use of organic manure and azolla on methane emission on paddy field of organic systems was conducted on paddy fields in the Gempol Village, Sambirejo District of Sragen Regency, Indonesia. The experimental design performed for this study was a completely randomized block design consisting of three factors; the factor I was rice cultivars (Mira-1; Mentik Wangi; Merah Putih; factor II was dose of organic manure (0 t/ha and 10 t/ha and factor III was Azolla inoculums dose (0 t/ha and 2 t/ha. Gas sampling was conducted 3 times in one growing season when the rice plants reached ages of 38, 66 and 90 days after planting. The results showed that there was no correlation between the uses of organic fertilizers for rice production on methane emission. The increase of methane emission was very much influenced by the redox potential. Methane emission from Mira-1 field was higher than that from Mentik Wangi and Merah Putih fields. Emission of methane gas from Mira-1 field ranged from -509.82 to 791.34 kg CH4/ha; that from Wangi ranged from -756.77 to d 547.50 kg CH4/ha and that from Merah Putih ranged from -399.63 to 459.94 kg CH4/ha. Application of 10 t organic manure /ha and 2 t azolla/ha in Mentik Wangi reduced methane emissions with a high rice production compared to Merah Putih and Mira-1.

  4. Agricultural production - Phase 2. Indonesia. Sources and sinks of nitrogen-E phosphorus-based nutrients in cropping systems

    International Nuclear Information System (INIS)

    This document is the report of an expert mission to assist in the initiation of research on sustainable agriculture in rice-based cropping systems as related to the flow of plant nutrients, and on the use of legumes in upland cropping systems. Experimental suggestions include an investigation of the acid tolerance of different soybean strains under upland conditions, an analysis of ways to replace fertilizer nitrogen for rice crops by a green manure such as azolla, and a study of the increase in nutrient availability due to th presence of fish in a paddy field

  5. Pteridophyta collected in Northern Nigeria and Northern Cameroon

    Directory of Open Access Journals (Sweden)

    Jan kornaś

    2014-02-01

    Full Text Available 25 species of Pteridophyta were collected in Northern Nigeria (mainly the Lake Chad Basin and the Mandara Mts. and in the neighbouring parts of Cameroon. 11 of them have not been recorded previously from this area: Isoetes schweinfurthii A. Br. in Bak., Selaginella tenerrima A. Br. ex Kuhn, Ophioglossum gomenzianum Welw. ex A. Br., Marsilea coromandeliana Willd., M. distorta A. Br., M. nubica A. Br., M. subterranea Lepr. ex A. Br., Azolla africana Desv., Ceratopteris richardii Brogn., Adiantum capillus-veneris Linn., and Actiniopleris semiflabellata Pic. Ser.

  6. The organic matter of the Potosi basin (Cordillera Oriental, Bolivia) during the Upper Cretaceous-Lower tertiary: stratigraphic and palaeogeographic data

    Energy Technology Data Exchange (ETDEWEB)

    Blanc-Valleron, M.M.; Rouchy, J.M. (Centre National de la Recherche Scientifique (CNRS), 75 - Paris (France)); Schuler, M.; Rauscher, R. (Strasbourg-1 Univ., 67 (France)); Camoin, G. (Aix-Marseille-1 Univ., 13 - Marseille (France))

    1994-12-01

    Palynological and Rock-Eval pyrolysis studies of the Chaunaca and El Molino Fm (Santonian to Thanetian, Cordillera Oriental, Bolivia) indicate that some facies have economic significance as potential oil source rocks. The occurrence of Pediastrum and Azolla testify a lacustrine environment. In the Upper Molino, the environmental interpretation of an almost monospecific association of dinocysts is discussed. The presence of Apectodinium quinquelatum indicates that the age of the upper part of the Upper El Molino Fm is likely to be Upper Thanetian. (authors). 22 refs., 5 figs.

  7. Soils Newsletter. V. 9, no. 1

    International Nuclear Information System (INIS)

    This Newsletter describes the opening of the Soils and Plant Breeding Laboratory at the IAEA Seibersdorf Laboratory and describes the research taking place in the new unit. The Research Co-ordination Meetings on the improvement of yield and nitrogen fixation of the common bean in Latin America (December 1985, CENA, Piracicaba, Brazil), the management of legumes in pastures (March 1986, IAEA, Vienna) and isotopic studies of nitrogen fixation and nitrogen cycling by blue-green algae and Azolla (May 1986, International Rice Research Institute, The Philippines) are summarized, and recent publications are briefly described. Tabs

  8. MORFOLOGIA EXTERNA DA OPERÁRIA DE Lestrimelitta ehrhardti (FRIESE, 1931 (HYMENOPTERA: MELIPONINI. Morfología externa de la obrera de Lestrimelitta ehrhardti (Friese, 1931 (Hymenoptera: Meliponini. Worker External Morphology of Lestrimelitta ehrhardti (Friese, 1931 (Hymenoptera: Meliponini.

    Directory of Open Access Journals (Sweden)

    DANIELE R. PARIZOTTO

    Full Text Available Operárias de Lestrimelitta ehrhardti (Friese, 1931 foram estudadas, a partir de um grande número de exemplares coletados no município de Castro, Paraná, Brasil. Os espécimes foram descritos morfologicamente, medidos e ilustrados.Se estudió un gran número de obreras de Lestrimelitta ehrhardti (Friese, 1931 colectadas en el municipio de Castro, Paraná, Brasil. Los especímenes fueran descritos morfológicamente, medidos e ilustrados.Workers of Lestrimelitta ehrhardti (Friese, 1931 were studied based on a great number of individuals collected in the municipality of Castro, Paraná State, Brazil. The specimens were described morphologically, measured and illustrated.

  9. Presencia de proteína transgénica y su efecto sobre el contenido de taninos y aflatoxinas en maíz comercial

    OpenAIRE

    Silvia Denise Peña Betancourt

    2013-01-01

    En México en el estado de Oaxaca se detectó por primera vez la contaminación de maíz nativo con maíz transgénico. Sin embargo, pocos estudios se han enfocado a evaluar el efecto de la contaminación transgénica sobre los taninos (insecticidas naturales) y la contaminación por aflatoxinas. En este trabajo se realizó un monitoreo de la contaminación transgénica en trece genotipos de maíz (diez híbridos y tres nativos). Las muestras fueron colectadas en los estados de Hidalgo (5), Morelos (2), Es...

  10. A new species of Callistethus from Mexico (Coleoptera, Melolonthidae, Rutelinae Una nueva especie de Callistethus de México (Coleoptera, Melolonthidae, Rutelinae

    Directory of Open Access Journals (Sweden)

    Andrés Ramírez-Ponce

    2012-06-01

    Full Text Available Callistethus tlapanecus, a new species collected in pine forest located at 2160 meters of altitude in eastern mountains of the state of Guerrero, Mexico is described. The morphological characters that distinguish it from other Mexican and Central American species are discussed. The diagnostic characters are illustrated, and a key to Mexican species of Callistethus is also included.Se describe Callistethus tlapanecus, nueva especie colectada en bosques de pino situados a 2160 metros de altitud en las montañas orientales del estado de Guerrero, México. Se comentan los caracteres morfológicos que distinguen a esta especie de otras especies mexicanas y centroamericanas de Callistethus. Se ilustran los caracteres diagnósticos y se incluye una clave para separar las especies mexicanas de Callistethus.

  11. Cinco nuevas especies de Ácaros Oribátidos (Acari, Oribatida de pinares incendiados de la Sierra de Gredos (Ávila, España

    Directory of Open Access Journals (Sweden)

    Gil-Martín, J.

    1996-12-01

    Full Text Available Five new species of Oribatid mites found in soil samples collected in the burned pine forests of several localities in the Sierra de Gredos (Province of Avila, Central Spain are described: Brachychthonius amicabilis n. sp., Hermanniella issanielloides n. sp., Ctenobelba pulchellula n. sp., Xenillus arilloi n. sp., and Carabodes gregorioi n. sp.

    En este trabajo se describen cinco nuevas especies de Ácaros Oribátidos, encontradas en muestras colectadas en suelos de pinares incendiados de varias localidades de la Sierra de Gredos (Provincia de Ávila, Centro de España: Brachychthonius amicabilis n. sp., Hermanniella issanielloides n. sp., Ctenobelba pulchellula n. sp., Xenillus arilloi n. sp., y Carabodes gregorioi n. sp.

  12. Genética de poplaciones de Atta sexdens rubropilosa (Hymenoptera: Formicidae) POPULATION GENETICS OF Atta sexdens rubropilosa (HYMENOPTERA: FORMICIDAE)

    OpenAIRE

    LIRIANA BELIZÁRIO CANTAGALLI; CLAUDETE APARECIDA MANGOLIN; MARIA CLAUDIA COLLA RUVOLO TAKASUSUKI

    2013-01-01

    La variabilidad genética de las hormigas Atta sexdens rubropilosa colectadas en cinco lugares distintos de Brasil fueron evaluados por la técnica PCR-RAPD. Un total de 15 primers produjeron 148 fragmentos, de los cuales 123 fueron polimórficos, lo que corresponden al 83,11 %. La estimación de la diversidad genética por el índice de Shannon fue 0,3836 y el desviación estándar fue de ± 0,2335. Estos valores demuestran una alta diversidad genética. El valor de G ST fue 0,2372 y ΦPT = 0,184 l...

  13. Caracterización polinica estacional de miel inmadura de Apis mellifera L. en el Estado de Tabasco

    OpenAIRE

    Castellanos Potenciano, Blanca Patricia

    2010-01-01

    La presente investigación es una contribución al conocimiento de la flora de interés apícola y a los hábitos de pecoreo de Apis mellifera L. basado en análisis melisopalinológicos de miel inmadura colectada de Febrero a Mayo del 2009, en las principales subregiones apícolas del estado de Tabasco: subregión Centro (municipio Centro); subregión Chontalpa (municipios Cárdenas y Paraíso); subregión Ríos (municipios Balancán y Tenosique) y subregión Sierra (municipio Tacotalpa). Se encontró que 29...

  14. Lista de Insectos Identificados para el Museo Entomológico de la Facultad de Ciencias Agrícolas U.N. Medellín, Durante los Años 1971 a 1973

    Directory of Open Access Journals (Sweden)

    Vélez Angel Raúl

    1973-12-01

    Full Text Available La presente catalogación de insectos presenta en forma resumida la mayor parte de información acumulada sobre material entomológico colectada durante excursiones del Área Entomología durante los años 1971 a 1973. No todo el material colectado ha sido identificado. La clasificación de insectos es una labor difícil y lenta, que ha sido en este caso realizada, en su mayoría, en forma gratuita por taxónomos extranjeros, particularmente del Museo Nacional de los Estados Unidos. Huella parte del material permanece preparado para posteriores envíos a entidades de reconocido prestigio y con los cuales han existido cordiales relaciones desde hace más de 30 años.

  15. A new afrotropical species of Ormyrus Westwood, 1832 (Hymenoptera, Chalcidoidea, Ormyridae

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    Gómez, J. F.

    2007-06-01

    Full Text Available Anew species, Ormyrus fernandinus sp. n. (Hymenoptera, Chalcidoidea, Ormyridae, is described from the island of Bioko (Equatorial Guinea. Collected with Malaise trap, the biology of the new species is unknown. The differential morphological features of the new species with respect to known afrotropical Ormyrus species, and its phylogenetic relationships are discussed.Se describe una nueva especie, Ormyrus fernandinus sp. n. (Hymenoptera, Chalcidoidea, Ormyridae, de la isla de Bioko (Guinea Ecuatorial. La especie fue colectada con trampa Malaise por lo que se desconoce su biología. Se discuten los caracteres distintivos fundamentales de la nueva especie en relación a las especies de Ormyrus conocidas en la region Afrotropical, y sus relaciones filogenéticas.

  16. PRIMER REPORTE DE Cryptosporidium parvum EN TERNEROS HOLSTEIN (Bos taurus DE MANIZALES, CALDAS, COLOMBIA4

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    R. J. Ocampo

    2012-01-01

    Full Text Available RESUMEN El objetivo de este estudio fue identificar especies o genotipos del protozoario parásito Cryptosporidium presentes en heces colectadas de terneros Holstein del municipio de Manizales, Departamento de Caldas, Colombia. El ADN fue extraído a 80 muestras de materia fecal, de las cuales 11 fueron diagnosticadas positivas para Cryptosporidium spp., mediante reacción en cadena de la polimerasa (PCR. El análisis PCR-RFLP del locus 18S ADNr, identificó la presencia de Cryptosporidium parvum en todas las muestras positivas analizadas. Este hallazgo sugiere que el ganado puede ser una fuente potencial de infección por Cryptosporidium en humanos y se constituye en el primer reporte publicado de C. parvum en bovinos de Manizales, Caldas.

  17. Fossilized glycolipids reveal past oceanic N2 fixation by heterocystous cyanobacteria.

    Science.gov (United States)

    Bauersachs, Thorsten; Speelman, Eveline N; Hopmans, Ellen C; Reichart, Gert-Jan; Schouten, Stefan; Damsté, Jaap S Sinninghe

    2010-11-01

    N(2)-fixing cyanobacteria play an essential role in sustaining primary productivity in contemporary oceans and freshwater systems. However, the significance of N(2)-fixing cyanobacteria in past nitrogen cycling is difficult to establish as their preservation potential is relatively poor and specific biological markers are presently lacking. Heterocystous N(2)-fixing cyanobacteria synthesize unique long-chain glycolipids in the cell envelope covering the heterocyst cell to protect the oxygen-sensitive nitrogenase enzyme. We found that these heterocyst glycolipids are remarkably well preserved in (ancient) lacustrine and marine sediments, unambiguously indicating the (past) presence of N(2)-fixing heterocystous cyanobacteria. Analysis of Pleistocene sediments of the eastern Mediterranean Sea showed that heterocystous cyanobacteria, likely as epiphytes in symbiosis with planktonic diatoms, were particularly abundant during deposition of sapropels. Eocene Arctic Ocean sediments deposited at a time of large Azolla blooms contained glycolipids typical for heterocystous cyanobacteria presently living in symbiosis with the freshwater fern Azolla, indicating that this symbiosis already existed in that time. Our study thus suggests that heterocystous cyanobacteria played a major role in adding "new" fixed nitrogen to surface waters in past stratified oceans. PMID:20966349

  18. Design of an integrated piggery system with recycled water, biomass production and water purification by vermiculture, macrophyte ponds and constructed wetlands.

    Science.gov (United States)

    Morand, Philippe; Robin, Paul; Pourcher, Anne-Marie; Oudart, Didier; Fievet, Sebastien; Luth, Daniel; Cluzeau, Daniel; Picot, Bernadette; Landrain, Brigitte

    2011-01-01

    Since 2001 the swine experimental station of Guernévez has studied biological treatment plants for nutrient recovery and water recycling, suited to the fresh liquid manure coming out of flushing systems. An integrated system with continuous recycling was set up in 2007, associated with a piggery of 30 pregnant sows. It includes a screen, a vermifilter, and macrophyte ponds alternating with constructed wetlands. The screen and the vermifilter had a lower removal efficiency than in previous studies on finishing pigs. A settling tank was then added between the vermifilter and the first lagoon to collect the worm casts. A second vermifilter was added to recover this particulate organic matter. A storage lagoon was added to compensate for evaporative losses and complete pollution abatement, with goldfish as a bioindicator of water quality. The removal efficiency of the whole system was over 90% for COD and nitrogen, over 70% for phosphorus and potassium, and more than 4 logarithmic units for pathogens (E. coli, enterococci, C perfringens). Plant production was about 20 T DM ha(-1) y(-1). Floating macrophytes (Azolla caroliniana, Eichhornia crassipes, Hydrocotyle vulgaris) were more concentrated in nutrients than helophytes (Phragmites australis, Glyceria aquatica,…). Azolla caroliniana was successfully added to feed finishing pigs. PMID:21436573

  19. Complex agro-ecosystems for food security in a changing climate.

    Science.gov (United States)

    Khumairoh, Uma; Groot, Jeroen Cj; Lantinga, Egbert A

    2012-07-01

    Attempts to increase food crop yields by intensifying agricultural systems using high inputs of nonrenewable resources and chemicals frequently lead to de-gradation of natural resources, whereas most technological innovations are not accessible for smallholders that represent the majority of farmers world wide. Alternatively, cocultures consisting of assemblages of plant and animal species can support ecological processes of nutrient cycling and pest control, which may lead to increasing yields and declining susceptibility to extreme weather conditions with increasing complexity of the systems. Here we show that enhancing the complexity of a rice production system by adding combinations of compost, azolla, ducks, and fish resulted in strongly increased grain yields and revenues in a season with extremely adverse weather conditions on East Java, Indonesia. We found that azolla, duck, and fish increased plant nutrient content, tillering and leaf area expansion, and strongly reduced the density of six different pests. In the most complex system comprising all components the highest grain yield was obtained. The net revenues of this system from sales of rice grain, fish, and ducks, after correction for extra costs, were 114% higher than rice cultivation with only compost as fertilizer. These results provide more insight in the agro-ecological processes and demonstrate how complex agricultural systems can contribute to food security in a changing climate. If smallholders can be trained to manage these systems and are supported for initial investments by credits, their livelihoods can be improved while producing in an ecologically benign way. PMID:22957173

  20. Salinity of the Eocene Arctic Ocean from oxygen isotope analysis of fish bone carbonate

    Science.gov (United States)

    Waddell, Lindsey M.; Moore, Theodore C.

    2008-03-01

    Stable isotope analysis was performed on the structural carbonate of fish bone apatite from early and early middle Eocene samples (˜55 to ˜45 Ma) recently recovered from the Lomonosov Ridge by Integrated Ocean Drilling Program Expedition 302 (the Arctic Coring Expedition). The δ18O values of the Eocene samples ranged from -6.84‰ to -2.96‰ Vienna Peedee belemnite, with a mean value of -4.89‰, compared to 2.77‰ for a Miocene sample in the overlying section. An average salinity of 21 to 25‰ was calculated for the Eocene Arctic, compared to 35‰ for the Miocene, with lower salinities during the Paleocene Eocene thermal maximum, the Azolla event at ˜48.7 Ma, and a third previously unidentified event at ˜47.6 Ma. At the Azolla event, where the organic carbon content of the sediment reaches a maximum, a positive δ13C excursion was observed, indicating unusually high productivity in the surface waters.

  1. Effect of Land Use Pattern on Mineralization of Residual C and N from Plant Materials Decomposing Under Field Conditions

    Institute of Scientific and Technical Information of China (English)

    CHENGLILI; WENQIXIAO

    1998-01-01

    Four kinds of plant materials(astragalus,azolla,rice straw and water hyacinth) were allowed to decom-pose for 10 years in two soils with different mineralogical characteristics in fields under upland and submerged conditions.Greater amounts of C and N from azolla were retained in soils throughout the 10-year experi-mental period compared to those from the other plant materials.The residual C of all the plant materials in the two soils under upland conditions mineralized at rates corresponding to half-lives between 4.4-6.6 years,while the corresponding figures for those under submerge conditions were between 6.5-13.1 years,Minera-liztion of residual organic N followed the same pattern as residual C.Compared to residual C,however,the mineralization rates of residual organic N in most cases were significantly lower and the percentages of added N retained in soils were higher.More N from plant materials was retained in the yellow-brown soil than in the red soil,but no consistent differences in the amounts of C from plant materials and in the mineraliztion rates of both residual C and residual organic N between the wto soils could be folund.

  2. The effect of type and ratio of vermicompost on selected growth indices and nutrients content of tomato at greenhouse conditions

    Directory of Open Access Journals (Sweden)

    M.H. Rasouli-Sadaghiani

    2016-02-01

    Full Text Available This study was conducted to investigate the effect of type and ratio of vermicompost on tomato growth, with five different types of vermicompost (platanus leaves, maple leaves, pruning apple trees and grape, waste of herbal extracts and azolla residues and four ratios of vermicompost to peat and perlite (2:1 v/v as 0, 1:3, 2:3 and 3:3, at greenhouse conditions. Results showed that type of vermicompost had a significant effect (P≤ 0.05 on plant height, shoot dry weight, root dry weight, number of internodes and stem diameter. The interaction between type and ratio of vermicompost had significant effect on root and shoot dry weight, stem diameter, nitrogen (N percent, and potassium (K percent. The highest effect on shoot dry weight, stem diameter and the N percentage was observed in the ratio of 2:3 vermicompost of azolla residues. The amount of K in the ratio of 2:3 vermicompost of maple leaves increased 66.18% as compared to the control treatment. Also, different ratios of vermicompost increased percentage of phosphorus and concentration of iron and zinc, as compared to peat+ perlite treatment. Generally, different types and ratios of vermicompost compared to peat+ perlite medium had a positive effect on growth indices and mineral concentration in the tomato plant.

  3. Presencia de Spiroplasma penaei en plancton, bentos y fauna acompañante en fincas camaroneras de Colombia

    Directory of Open Access Journals (Sweden)

    José Altamiranda M.

    2011-05-01

    Full Text Available Objetivo. Determinar la presencia de Spiroplasma penaei en el plancton, bentos y fauna acompañante en 2 fincas comerciales de camarones. Materiales y métodos. Fueron colectadas 200 muestras para identificación de lesiones características de Spiroplasma, a través de la técnica de histología, mientras que para la técnica de PCR se tomaron 326 muestras de plancton, bentos y fauna acompañante. Las muestras colectadas fueron preservadas en tubos estériles con etanol al 95% para análisis de PCR y en solución Davidson para análisis histológicos. Resultados. En los muestreos realizados en las dos fincas camaroneras fue detectada la presencia de Spiroplasma en una muestra de un representante de los dípteros (mosca de agua a través de la técnica de PCR en tiempo real, el cual arrojo una Tm=84, similar a la del control positivo de Spiroplasma utilizado. Esta muestra fue secuenciada y comparada con secuencias de bacterias almacenadas en el banco de datos GenBank usando el algoritmo BLAST, encontrando 100% de homología con un fragmento de los genes ribosomales 16S de la bacteria Spiroplasma penaei. Conclusiones. La mosca de agua es portadora de Spiroplasma penaei, sin embargo no se puede afirmar que este organismo sea el transmisor de la infección, por lo que se recomienda realizar ensayos de tipo experimental con moscas de agua infectadas con Spiroplasma penaei, en camarones libres de patógenos, para evaluar si en realidad es el vector de infección.

  4. Análisis fitoquímico y farmacológico de extractos de Allophylus cominia (L. Sw

    Directory of Open Access Journals (Sweden)

    Tania Véliz Rodríguez

    2004-01-01

    Full Text Available El efecto sobre la glicemia provocado por la administración oral de los extractos acuoso y alcohólico de la planta Allophylus cominia (L.Sw (Palo de caja, 1g/kg m.c., colectada en San José de las Lajas y Nueva Paz, fue estudiado en ratas normoglicémicas, sin observarse diferencias significativas entre estos grupos. En el ensayo con sobrecarga de glucosa (PTG, el extracto acuoso de las hojas fue el que presentó un efecto biológico sobre las concentraciones séricas de glucosa, a diferencia del de los tallos y la corteza para este modelo farmacológico, mientras que el estudio fitoquímico, indicó una misma composición química general para todos los extractos analizados. Finalmente, la evaluación de la toxicidad aguda oral tras la administración de una dosis de 5 000 mg/kg. m.c. del extracto acuoso de las hojas colectadas en San José de las Lajas, reveló que, bajo las condiciones experimentales, la sustancia ensayada no provoca alteraciones que pudieran interpretarse como tóxicas, como se había observado previamente para el caso del extracto acuoso de la corteza. Estos resultados permiten afirmar que el consumo de decocciones de hojas de Allophylus cominia (L.Sw, además de constituir una ventaja desde el punto de vista medioambiental, no provoca efecto sobre la glucosa sanguínea en modelos normoglicémicos bajo las condiciones experimentales empleadas. Se debe valorar el resultado de su administración en dosis repetidas en modelos diabéticos, en concordancia con su uso etnomédico.

  5. Detección de Rickettsia spp. en Garrapatas de Myrmecophaga tridactyla de Vida Libre en la Sabana Inundable de Casanare‚ Colombia -resumen-

    Directory of Open Access Journals (Sweden)

    César Rojano-Bolaño

    2014-12-01

    Full Text Available Las bacterias del género Rickettsia son organismos con distribución mundial‚ causantes de algunas enfermedades zoonóticas de gran importancia en salud pública. Sin embargo‚ en Colombia son pocos los estudios enfocados a conocer su distribución y los vectores involucrados. El objetivo de este estudio fue detectar la presencia de Rickettsia spp.‚ en garrapatas colectadas en nueve osos palmeros (Myrmecophaga tridactyla de vida libre en el municipio de Pore‚ Casanare‚ entre los años 2013 y 2014. Los osos palmeros fueron capturados con el método de búsqueda activa‚ para posteriormente ser anestesiados utilizando un dardo con una combinación anestésica consistente en Ketamina 12 mg/kg y Xilacina 0‚5 mg/kg por vía intramuscular. Las garrapatas fueron colectadas directamente de los individuos‚ con ayuda de pinzas. En total se obtuvieron 204 ectoparásitos‚ que fueron almacenados en alcohol al 70%‚ y posteriormente fueron identificados como pertenecientes a la especie Amblyomma cajennense. Las garrapatas se organizaron en 68 pool de tres individuos y fueron analizadas por medio de la técnica de PCR en tiempo real. Se detectaron 12 pool de garrapatas positivos a este microorganismo (17‚6%. Los resultados preliminares de este estudio muestran que Rickettsia spp.‚ bacteria intracelular importante en salud pública‚ se encuentra circulante entre ectoparásitos de animales silvestres en la sabana inundable del municipio de Pore‚ Casanare. Dado que diversos estudios han reportado que los rickettsiales tienen una mayor incidencia en ambientes húmedos‚ se recomienda continuar con los estudios en mamíferos silvestres y sus garrapatas en la sabana natural inundable.

  6. NUEVO REGISTRO DE Greenidea ficicola Takahashi (Hemiptera: Sternorrhyncha: Aphididae EN GUAYABO Psidium guajava (Myrtaceae EN ANTIOQUIA, COLOMBIA NEW REGISTRATION OF Greenidea ficicola Takahashi (Hemiptera: Sternorrhyncha: Aphididae IN GUAVA Psidium guajava (Myrtaceae IN ANTIOQUIA, COLOMBIA

    Directory of Open Access Journals (Sweden)

    Rubén Darío David Giraldo

    2009-06-01

    Full Text Available Se encuentra por primera vez en el departamento de Antioquia (Colombia y asociado al guayabo (Psidium guajava L., la especie Greenidea ficicola Takahashi ( Hemiptera: Sternorrhyncha: Aphididae colectada durante muestreos intensivos realizados durante el primer semestre de 2008 en el Valle de Aburrá y municipios aledaños. Dicha especie de áfido también fue colectada en falso laurel (Ficus benjamina. Dada la importancia que tienen los frutos de guayabo en la alimentación humana y de los animales domésticos en Colombia, es necesario registrar esta especie con el fin de tomar medidas en lo referente a programas de muestreo de sus poblaciones, estudio de su ciclo de vida y determinar los posibles controladores biológicos, que conduzcan al mejor conocimiento de este insecto y faciliten la toma de medidas de contención a su dispersión en el país.It is founded for the first time, the species of aphid Greenidea ficicola, Takahashi (Hemiptera: Sternorrhyncha: Aphididae in the Antioquia Department (Colombia associated to guava plants (Psidium guajava L.. The species was collected during intensive sampling done during the first semester of 2008, in the Aburra Valley and near municipalities. The species of aphid was also collected on "false laurel" (Ficus benjamina. Due to the importance of guava as food for humans as well as wild and domestic animals, it is considered important to report this species so that contention measures can be taken specially related with more sampling, life cycle and potential biological control agents to get to know better this pest and control its spread in the country.

  7. Genetic analysis of greenbug populations of Schizaphis graminum (Hemiptera: Aphididae from Argentina and Chile based on enzyme variability

    Directory of Open Access Journals (Sweden)

    Luciana SALDÚA

    2011-01-01

    Full Text Available Veintitrés poblaciones de Schizaphis graminum (Rondani y sesenta clones colectados en regiones muy contrastantes de la Argentina y Chile fueron investigadas electroforéticamente. Se encontró un alto grado de polimorfismo enzimático. La estructura enzimática fue descripta para el sistema estearasa y se encontraron nueve loci diferentes. Se determinó que existe estratificación latitudinal, las poblaciones fueron asociadas en tres grupos de acuerdo a la latitud donde fueron colectadas. El 90% de los loci resultaron polimórficos en el primer grupo y el 100% de los loci lo fueron en el resto. La heterocigosidad observada fue menor que la esperada. Ningún alelo fue fijado, de acuerdo con el valor del Fsr. El primer grupo tuvo un ligero exceso de heterocigosis, mientras que los demás grupos mostraron valores ligeramente positivos de Fsr, debido al exceso de homocigosis. Con respecto a Frt, el tercer grupo mostró un valor alto que estuvo en concordancia con el alto valor de flujo génico. Tres poblaciones no pudieron ser incluidas en ningún grupo. Las α-carboxil- estearasas siempre estuvieron presentes incluso en clones y poblaciones colectadas en zonas no agrícolas, lo que implica que la resistencia a insecticidas está ampliamente extendida a lo largo del territorio argentino y chileno. No se encontró relación entre el patrón enzimático y el biotipo, así como tampoco con la especie hospedera de donde fueron colectados los áfidos.

  8. 新食物链生态系统中水流速度对净化效果的影响%Effect of Flow Velocity on Decontamination of Aquaculture Circulating Water in New Food Chain Ecological System

    Institute of Scientific and Technical Information of China (English)

    陈敏; 杨有泉; 邓素芳; 刘中柱

    2011-01-01

    利用不同流量的循环水流经新食物链生态系统中的栽培层架,研究水流速度对循环水净化效果,旨在揭示新食物链生态系统节水和减排的本质.试验结果表明,流经各单层红萍的水样中溶解氧(DO)含量的上升幅度为0.76~0.96mg·L,铵态氮(NH-N)含量的下降幅度为0.94-1.19mg·L,分别是流经单层莴苣的5.07~6.40倍和1.71-2.16倍,说明红萍对提高水体中DO含量和去除NH-N的效果均明显优于莴苣.当养殖循环水流量控制在50mL·s时,流经1层莴苣和3层红萍组成的单个植物栽培床后,DO含量上升了2.80mg·L,系统增O总量为504.0mg·h;NH-N含量下降了3.82mg·L,系统NH-N去除总量为687.6mg·h.可见,红萍是新食物链生态系统的关键植物,在水质净化中起重要作用.利用红萍等植物改善水产养殖水环境,为解决高密度集约化水产养殖的瓶颈问题提供新途径,真正意义上实现新食物链生态系统中养殖污水零排放.%In order to reveal the essence of water conservation and emission reduction in New Food Chain ecological shed, different current water was flew through the cultivation shelves for studying the effect of water flow velocity on purification. The results showed that using Azolla to decontaminate, the concentration of dissolved oxygen (DO) in the water increased by 0.76 ~ 0. 96 mg · L-1 per layer, the concentration of ammonium nitrogen ( NH4+ - N) decreased by 0.94 ~ 1.19mg · L-1 per layer, was 5.07 ~6.40 times and 1.71 ~2. 16 times of using lettuce respectively, which indicated that the effect of Azolla on increasing DO and removing NH4+ - N was much better than lettuce. Controlled the water flow at 50mL · s- 1, after water flew through one layer of lettuce and three layers of Azolla, the concentration of DO increased by 2.80mg · L-1, the total content of O2 increasing of the system was 504.0mg · h -1, the concentration of NH4+ -N decreased by 3.82mg · L-1, the total content of

  9. 常见水生植物对农田退水中总氮和总磷的净化能力研究%Removal Ability of Common Aquatic Plants on Total N and Total P in Farmland Drainage

    Institute of Scientific and Technical Information of China (English)

    刘作云; 王雅丽; 龚道新; 焦雨婷

    2012-01-01

    为了解常见水生植物对农田退水中总氮(TN)和总磷(TP)的净化作用,通过模拟实验研究了空心菜、紫背浮萍、水芹菜、水浮莲、三叶浮萍和满江红对农田退水中TN和TP的净化能力.结果表明:在处理20 d后,供试水生植物对农田退水中TN的净化率达85.8%~95.6%,净化率大小顺序为:空心菜>水芹菜>水浮莲≈紫背浮萍>满江红≈三叶浮萍;TP的净化率达95.3%~97.2%,净化率大小顺序为:水芹菜>空心菜>水浮莲>紫背浮萍≈三叶浮萍≈满江红.而对照组仅为55.6%(TN)和58.9%(TP).这说明6种供试水生植物对农田退水中TN和TP均有较强的净化能力.%In order to research the removal ability of common aquatic plants on TN and TP in farmland drainage, using stimulated experiment to study the removal ability of Ipomoea aquatica Forsk, Spirodela polyrrhiza, Oenanthe javanica, Eichhomia crassipes, Lemma paucicostata and Azolla imbircata on TN and TP in farmland drainage. The results showed that 20 d after treatment applied, these six aquatic plants all had great. Removal ability on TN and TP in farmland drainage. The removal rates of them on TN were 85.8% ~95.6% with a order of Ipomoea aquatica Forsk >0enanthe javanica>Eichhornia crassipes ≈ Spirodela polyrrhiza>Azolla imbircata ≈ Lemma paucicostata, and the removal rates of them on TP were 95.3%-97.2% with a order of Oenanthe javanica>Ipomoea aquatica Forsk>Eichhomia crassipes > Spirodela polyrrhiza ≈ Lemma paucicostata ≈ Azolla imbircata, while the removal rate of control was only 55.6% on TN and 58.9% on TP.

  10. Purification of Eutrophic Water of Different Degrees with Hydrophytes%水生植物对不同富营养化程度水体净化能力研究

    Institute of Scientific and Technical Information of China (English)

    胡萃; 刘强; 龙婉婉; 郭琳; 廖国平; 周松

    2011-01-01

    为不同富营养化程度水体筛选出净化能力强的植物种类,文章采用模拟人工湿地系统处理人工配制污水的方法,以7d为一个采样周期,比较了5种植物(满江红、槐叶萍、四叶萍、黑藻和慈姑)在轻度、中度和重度富营养水体中TN、TP、NH4+-N去除能力.实验结果表明:5种植物处理能力随着富营养程度提高而增大.在重度富营养条件下,处理能力满江红>黑藻>四叶萍>槐叶萍>慈姑.在重度条件下,满江红对TN、TP、NH4+-N的平均去除率分别为94.51%、77.41%和74.64%.%To select better purification plants for eutorphic water of different degrees, the simulated constructed wetland system was used to treat sewage artificially made. Removal abilities of TP, TN, NIV-N by five hydrophytes including Azolla imbircata(Roxb.) Nakai, Salvinia natansiL.) All., Marsilea quadrifolia L, Hydrilla verticillata, Sagittaria sagittifolia in mildly, moderately and seriously eutropnic water were compared in the sampling period of 7d. Results showed that removal ability by these five hydrophytes increased with the improvement of the degree of eutropnic water, with removal ability ranking as Azolla imbircataiRoxb.) Nakai, Hydrilla verticillata, Marsilea quadrifolia L, Salvinia natansCL.) All. And Sagittaria sagittifolia in seriously eutrophic water. Removal rates of TN, TP, NIV-N by Azolla imbircata (Roxb.) Nakai were 94.51%, 77.41% and 74.64% respectively.

  11. An atmosphere-ocean GCM modelling study of the climate response to changing Arctic seaways in the early Cenozoic.

    Science.gov (United States)

    Roberts, C. D.; Legrande, A. N.; Tripati, A. K.

    2008-12-01

    The report of fossil Azolla (a freshwater aquatic fern) in sediments from the Lomonosov Ridge suggests low salinity conditions occurred in the Arctic Ocean in the early Eocene. Restricted passages between the Arctic Ocean and the surrounding oceans are hypothesized to have caused this Arctic freshening. We investigate this scenario using a water-isotope enabled atmosphere-ocean general circulation model with Eocene boundary conditions including 4xCO2, 7xCH4, altered bathymetry and topography, and an estimated distribution of Eocene vegetational types. In one experiment, oceanic exchange between the Arctic Ocean and other ocean basins was restricted to two shallow (~250 m) seaways, one in the North Atlantic, the Greenland-Norwegian seaway, and the second connecting the Arctic Ocean with the Tethys Ocean, the Turgai Straits. In the restricted configuration, the Greenland-Norwegian seaway was closed and exchange through the Turgai Straits was limited to a depth of ~60 m. The simulations suggest that the severe restriction of Arctic seaways in the early Eocene may have been sufficient to freshen Arctic Ocean surface waters, conducive to Azolla blooms. When exchange with the Arctic Ocean is limited, salinities in the upper several hundred meters of the water column decrease by ~10 psu. In some regions, surface salinity is within 2-3 psu of the reported maximum modern conditions tolerated by Azolla (~5 psu). In the restricted scenario, salt is stored preferentially in the North Atlantic and Tethys oceans, resulting in enhanced meridional overturning, increased poleward heat transport in the North Atlantic western boundary current, and warming of surface and intermediate waters in the North Atlantic by several degrees. Increased sensible and latent heat fluxes from the North Atlantic Ocean, combined with a reduction in cloud albedo, also lead to an increase in surface air temperature of over much of North America, Greenland and Eurasia. Our work is consistent with

  12. Bioenergy potential of eight common aquatic weeds

    Energy Technology Data Exchange (ETDEWEB)

    Abbasi, S.A.; Nipaney, P.C.; Schaumberg, G.D. (Pondicherry (Central) Univ. (IN). Salim Ali School of Ecology)

    1990-01-01

    Eight common aquatic weeds Salvinia molesta, Hydrilla verticillata, Nymphaea stellata, Azolla pinnata, Ceratopteris sp. Scirpus sp. Cyperus sp, and Utricularia reticulata were digested anaerobically to produce methane. The carbon to nitrogen (C/N) ratio, carbon to phosphorus (C/P) ratio, and the volatile solids (VS) content of the weeds varied widely. No trend between these factors and the methane yield was discernable; the possible reasons are discussed. The energy potential of the weeds per unit area of the weed crop was worked out. Natural stands of salvinia, such as the one employed in the present investigation, would yield energy (methane) of the order of 10{sup 8} Kcal/ha/yr. (author).

  13. Early pleistocene sediments at Great Blakenham, Suffolk, England

    Science.gov (United States)

    Gibbard, P. L.; Allen, P.; Field, M. H.; Hallam, D. F.

    Detailed investigation of a fine sediment sequence, the College Farm Silty Clay Member, that overlies the Creeting Sands (Early Pleistocene) in Suffolk, is presented. The sedimentary sequence is thought to represent a freshwater pool accumulation in a small coastal embayment. Palaeobotanical investigation of the sediment indicates that it accumulated during the late temperate substage of a temperate (interglacial) event. The occurrence of Tsuga pollen, associated with abundant remains of the water fern Azolla tegeliensis indicate that the deposits are of Early Pleistocene age and are correlated with a later part of the Antian-Bramertonian Stage. Correlation with Tiglian TO substage in The Netherlands' sequence is most likely. The sediments' normal palaeomagnetic polarity reinforces the biostratigraphical correlation.

  14. Effect of different long-term fertilizing practices on nitrogen mineralization characteristic of paddy soil%长期不同施肥对水稻土有机氮素矿化特性影响的研究

    Institute of Scientific and Technical Information of China (English)

    张玉玲; 张玉龙; 虞娜; 姬景红

    2008-01-01

    通过定位试验研究了长期不同施肥措施对水稻土氮素矿化特性的影响.结果表明,长期不同施肥措施显著影响水稻土氮矿化势及矿化速率.与不施氮肥相比,单施氮肥使水稻土氮矿化势下降,矿化速率加快;氮肥与有机肥配施可极显著增加水稻土氮矿化势,降低矿化速率.在氮肥与有机肥配施的基础上,水稻插秧后接种"Azolla"固氮菌体可降低氮矿化势,提高矿化速率.

  15. Isolation of non-sulphur photosynthetic bacterial strains efficient in hydrogen production at elevated temperatures

    Energy Technology Data Exchange (ETDEWEB)

    Singh, S.P.; Srivastava, S.C. (Banaras Hindu Univ., Varanasi (IN). Centre of Advanced Study in Botany)

    1991-01-01

    Four strains of non-sulphur photosynthetic bacteria were isolated from root zone associations of aquatic plants like Azolla, Salvinia and Eichhornia, as well as the deep-water rice. Based on the gross cell morphology and pigmentation, the isolates resembled Rhodopseudomonas sp. and have been designated as BHU strains 1 to 4, respectively. When subjected to elevated temperature (from 33-45{sup o}C), substantial growth/hydrogen production could be observed only in strains 1 and 4. Strains 2 and 3 on the other hand, showed diminished growth and negligible hydrogen photoproduction. The BHU strains 1 and 4 have been selected as the most active (thermostable) hydrogen producing strains of local origin as far as the Indian tropical climate is concerned. (author).

  16. Vascular Flora of the Chouteau Wildlife Management Area Wagoner County, Oklahoma Bruce W.

    Directory of Open Access Journals (Sweden)

    Bruce W. Hoagland

    2004-12-01

    Full Text Available This article reports the results of a vascular plant inventory of the Chouteau Wildlife Management Area in eastern Oklahoma. Oklahoma Native Plant Record Journal of the Oklahoma Native Plant Society One hundred eighty-one species of vascular plants were collected from 144 genera and 63 families. The families with the greatest number of species were the Asteraceae (25, Poaceae (22, and Fabaceae (18. Fifty-seven species were annuals, four biennials, and 120 were perennials. Thirty-nine woody plant species were present. Twenty-one species exotic to North America were collected representing 11.6% of the flora. Azolla caroliniana was the only species tracked by the Oklahoma Natural Heritage Inventory found. This study reports 148 species previously not documented in Wagoner County.

  17. Inoculant production in developing countries - Problems, potentials and success

    International Nuclear Information System (INIS)

    Sustainable agriculture is a long-term goal that seeks to overcome some of problems and constraints that confront the economic viability, environmental soundness and social acceptance of agricultural production systems. In this context, bio-fertilizers assume special significance particularly because they are 'eco-friendly', but also since their alternative, chemical fertilizers are expensive. Undoubtedly, the most commonly used bio-fertilizers are soil bacteria of the genus Rhizobium, but others like Azolla, Azospirillum, various cyanobacteria also contribute significant amounts of N to e.g. rice. Other bacteria like Frankia and Acetobacter contribute N to trees of the genus Casuarina and sugarcane, respectively. Furthermore, although they are rarely used as inoculants, vesicular arbuscular mycorrhizae (VAM) and phosphobacteria help countless plants solubilise and assimilate soil phosphorus. Despite these advantages, bio-fertilizers could be more widely used in developing countries. Contingent upon greater use is improved quality of the inoculants, and all aspects of their production are discussed here. (author)

  18. Effects of nitrogen fertilizer and pesticide management on floodwater ecology in a wetland ricefield : 1. Experimental design and dynamics of the photosynthetic aquatic biomass

    OpenAIRE

    Simpson, I.C.; Roger, Pierre-Armand; Oficial, R.; I. F. Grant

    1994-01-01

    Cet article est le premier d'une série présentant les résultats d'une étude des effets de l'application d'engrais azoté minéral (urée), d'engrais azoté organique (Azolla) et de pesticides sur l'écologie de l'eau de submersion d'un rizière tropicale. La dynamique des populations de cyanobactéries, de microalgues, du zooplancton et des mollusques a été étudiée dans des parcelles expérimentales soumises à différentes méthodes de fertilisation azotée et d'application de pesticides. L'article prés...

  19. 五种水生植物对水中铀的去除作用%Uranium removal from water by five aquatic plants

    Institute of Scientific and Technical Information of China (English)

    胡南; 丁德馨; 李广悦; 王永东; 李乐; 郑济芳

    2012-01-01

    采用水培实验,研究了浮叶植物野生水葫芦(Eichhornia crassipes)、漂浮植物浮萍(Lemna minor L)、满江红(Azolla imbircata)、沉水植物菹草(Potamogeton crispus)、挺水植物空心莲子草(Alligator Alternanthera Herb)在初始铀浓度分别为0.15、1.50和15.00mg·L-1水中的生长状况及它们对水中铀的去除能力.结果表明,在21d的水培试验期内,满江红对铀表现出了最强的抗性,0.15、1.50和15.00mg·L-1的铀对满江红的生长抑制率分别只有4.56%、2.48%和6.79%,而满江红对水中铀的去除率分别达到了94%、97%和92%.进一步的试验表明,每1L水中种植7.5g满江红,可以获得最大的铀去除率,将初始铀浓度为1.25、2.50、5.00和10.00mg·L-1的水体降至国家排放标准(GB23727-2009)规定值(0.05mg·L-1)以下分别需要17、19、23和25d.研究结果为进一步开展铀污染水体植物修复的研究打下了基础.%Hydroponic solution culture experiments were conducted on the growth of Eichhornia crassipes,Lemna minor L,Azolla imbircata,Potamogeton crispus,and Alligator alternanthera Herb in water with 0.15,1.50 and 15.00 mg · L-1 concentrations of uranium.The uranium removal from the water by the aquatic plants was also examined.For the 21 days of hydroponic solution culture experiments,Azolla imbircata exhibited the strongest resistance to uranium and its growth inhibition rates induced by the water with 0.15,1.50 and 15.00 mg · L-1 concentrations of uranium were 4.56%,2.48%,6.79%,respectively.The uranium removal rates from the water by the plant amounted to 94%,97% and 92%,respectively.Further experiments revealed that the most uranium removal could be achieved when 7.5 g Azolla imbircata was grown in 1 L of water.17,19,23 and 25 days were required for the plant with the uranium concentration in water of 1.25,2.50,5.00 and 10.00 mg · L-1 to reduce to below the national emission standards of China,respectively.The results

  20. Potential of macrophytes for removing atrazine from aqueous solution Potencial de macrófitas para remoção de atrazine de solução aquosa

    Directory of Open Access Journals (Sweden)

    F.P Guimarães

    2011-01-01

    Full Text Available The potential of three macrophytes, Azolla caroliniana, Salvinia minima, and Lemna gibba was assessed in this study to select plants for use in environmental remediation contaminated with atrazine. Experiments were carried out in a greenhouse over six days in pots containing Hoagland 0.25 strength nutritive solution at the following atrazine concentrations: 0; 0.01; 0.1; 1.0; 10.0 mg L-1. Decrease in biomass accumulation was observed in the three macrophytes, as well as toxic effects evidenced by the symptomatology developed by the plants which caused their deaths. The chlorosis and necrosis allowed to observe in the plants the high sensitivity of the three species to the herbicide. Plants presented low potential for removal of atrazine in solution when exposed to low concentrations of the herbicide. However, at the 10.0 mg L-1 atrazine concentration, L. gibba and A. caroliniana showed potential to remove the herbicide from the solution (0.016 and 0.018 mg atrazine per fresh mass gram, respectively. This fact likely resulted from the processes of atrazine adsorption by the dead material. The percentage of atrazine removed from the solution by the plants decreased when the plants were exposed to high concentrations of the pollutant. Azolla caroliniana, S. minima, and L. gibba were not effective in removing the herbicide from solution. The use of these species to remedy aquatic environments was shown to be limited.Avaliou-se, neste estudo, o potencial de três macrófitas - Azolla caroliniana, Salvinia minima e Lemna gibba - com vistas à seleção de plantas para remediação de ambientes contaminados por atrazine. Foram realizados experimentos em casa de vegetação durante seis dias, em vasos contendo solução nutritiva Hoagland (0,25 de força iônica, nas seguintes concentrações de atrazine: 0; 0,01; 0,1; 1,0; e 10,0 mg L-1. A redução da biomassa acumulada pelas macrófitas foi observada, bem como os efeitos de toxidez evidenciados pela

  1. Hydrogen photo-evolution by Rhodopseudomonas palustris 6A using pre-treated olive mill wastewater and a synthetic medium containing sugars

    International Nuclear Information System (INIS)

    Highlights: • Adsorbent matrices to convert fresh olive mill wastewater (OMWF) in feedstock. • Dry-Azolla and granular active carbon for adsorbing polyphenols from OMWF. • Photofermentative processes for biohydrogen production. • Culture mixing by means of an impeller or a magnetic stir bar. • A 30% of dephenolised OMW containing medium suits the photofermentative process. - Abstract: Increasing costs of petroleum, associated with the escalating problems of global climate change, require always greater efforts in order to produce an energy carrier as bioH2. In this study, bioH2 production using photofermentative process was investigated. Two culture broths were used: (a) a synthetic medium rich in sugars (glucose and fructose) and (b) a pre-treated fresh olive-mill wastewater (OMWF) diluted with water (30%, v:v). The pre-treatment was carried out using two different vegetable matrices (dry-Azolla and granular active carbon) to decrease both the content of polyphenols and the dark colour of wastewater. Rhodopseudomonas palustris 6A isolated from soil spread with OMW was utilized for batch growth experiments, carried out indoors under continuous light (200 μE/m2/s). When synthetic medium was used, the culture mixing was performed using either (i) a magnetic stir bar, and (ii) an impeller equipped with five turbines. The latter system made it possible to increase the bioH2 photo-evolution by 1.4 times. The specific hydrogen photo-evolution rate was 13.5 mL/g(dw)/h in the broth containing diluted OMWF and 11.8 mL/g(dw)/h in the synthetic medium containing sugars (glucose and fructose)

  2. Modelado computacional usando regresión lineal y simbólica a B para determinar ópticamente el tamaño del poro de la piel

    Directory of Open Access Journals (Sweden)

    N. P. Puente Ramírez

    2015-01-01

    Full Text Available La apariencia de los poros de la piel normalmente es causada por glándulas sebáceas hiperactivas, sin embargo, en los últimos años se ha reportado que además se ve afectada por diversas enfermedades, entre ellas la diabetes [1], obesidad [2] o cáncer [3]. Generalmente los principales factores que contribuyen a la dilatación de los poros son la edad, género, acné, la exposición crónica al sol o la predispocisión genética. El desarrollo de un método no invasivo que permita conocer las condiciones de la piel, ayudara a relacionar los factores que determinan el tamaño del poro de la piel. En este trabajo se pretende caracterizar por medio de un modelo computacional y con ayuda de un esquema óptico, el tamaño del poro de la piel. El principio físico consiste en iluminar con leds (543nm el antebrazo de voluntarios, el esparcimiento de luz generado por la piel es colectada en una camara ccd. La captura de imágenes, el procesamiento y el análisis estadístico son parte de la metodología para la obtención de datos. Se propone un modelo matemático que relaciona género, edad, tono de piel y tamaño del poro (calculado estadísticamente en las imágenes colectadas ópticamente. Una base de datos se genera y se utiliza para construir matemáticamente modelos mediante regresión simbólica con optimizacion por enjambre de particulas (PSO y se realiza una comparación por medio de regresión lineal. Algunos indicadores estadísticos como el cuadrado medio de error, error de predicción suma de los cuadrados y porcentaje de variabilidad se utilizan en la comparación. Los resultados indican que el modelo propuesto con el tamaño de los poros de cada individuo puede ayudar a hacer una interpretación objetiva de indicadores de enfermedad que afecten directamente el tamaño del poro en la piel.

  3. Postlarval settlement of spiny lobster, Panulirus argus (Latreille, 1804 (Decapoda: Palinuridae, at the Caribbean coast of Costa Rica Asentamiento postlarval en la langosta espinosa, Panulirus argus (Latreille, 1804 (Decapoda: Palinuridae en la costa Caribe de Costa Rica

    Directory of Open Access Journals (Sweden)

    Oscar González

    2011-11-01

    Full Text Available Lobster fishery (Panulirus argus forms an important part of the fishing activities along the Caribbean coast of Central America. The present study provides information regarding the seasonal abundance and distribution of postlarval P. argus in Parque Nacional Cahuita, Costa Rica. During the study period (March 2004-February 2005, a total of 1907 pueruli were obtained from GuSi collectors. Postlarvae were present during all months, with a pronounced peak in January-February 2005 (CPUE of 21.82 and 22.18, pueruli/collector/month, respectively, and minor peaks in May and October 2004. The abundance of postlarval P. argus in the study area was comparable to locations which support important lobster fisheries, e.g. Mexico. A majority of the postlarvae (1027 ind. was collected during the first quarter moon, the remaining pueruli (880 ind. during new moon; these results are in general agreement with similar findings for P. argus in the Caribbean area. Based upon our results, we recommend introducing a local or regional monitoring program, studying spiny lobster migration and distribution patterns, and evaluate the introduction of artificial shelters for P. argus.Las capturas de langosta (Panulirus argus son de gran importancia en la actividad pesquera a lo largo de las costas del Caribe de Centroamérica. El presente estudio proporciona información relevante de la abundancia temporal y la distribución de postlarvas de P. argus en el Parque Nacional Cahuita, Costa Rica. Durante el periodo de estudio (Marzo 2004-Febrero 2005, un total de 1907 puérulos fueron obtenidos del colector GuSi. Las postlarvas fueron colectadas durante todos los meses, con máximos en enero-febrero 2005 (CPUE de 21.82 y 22.18, puérulos/colector/mes, respectivamente, y mínimos en mayo y octubre de 2004. La abundancia de postlarvas de P. argus en el estudio es comparada con áreas que soportan importantes pesquerías, p. ej. México. La mayoría de las postlarvas (1027 ind

  4. Detección de Anaplasma spp. en Garrapatas de Myrmecophaga tridactyla de Vida Libre en la Sabana Inundable de Casanare‚ Colombia -resumen-

    Directory of Open Access Journals (Sweden)

    César Rojano-Bolaño

    2014-12-01

    Full Text Available La anaplasmosis es una enfermedad infecciosa causada por rickettsias del género Anaplasma spp. Su relevancia esta principalmente determinada por el grado de afectación endémica en el ganado doméstico. Una amplia variedad de mamíferos silvestres también son susceptibles a la infección. Las bacterias pueden ser intracitoplasmáticas‚ dañando las estructuras de las células pudiendo causar anemia e ictericia. Algunas especies de Anaplasma spp han sido reportadas como microorganismos potencialmente zoonóticos‚ por lo tanto‚ la anaplasmosis‚ como otras enfermedades transmitidas por garrapatas‚ ha tomado una especial relevancia en la salud pública. El objetivo de este estudio fue detectar la presencia de Anaplasma spp.‚ en garrapatas colectadas en nueve osos palmeros (Myrmecophaga tridactyla de vida libre en el municipio de Pore‚ Casanare‚ entre los años 2013 y 2014‚ capturados con el método de búsqueda activa‚ y posteriormente fueron anestesiados utilizando un dardo con Ketamina (12 mg/kg y Xilacina (0‚5 mg/kg por vía intramuscular. Las garrapatas fueron colectadas directamente de los individuos‚ con ayuda de pinzas. En total se obtuvieron 204 ectoparásitos‚ que fueron almacenados en alcohol al 70%‚ y posteriormente fueron identificados como pertenecientes a la especie Amblyomma cajennense. Para este estudio se seleccionaron 55 pool de tres individuos y fueron analizadas por medio de la técnica de PCR en tiempo real. Se detectaron 2 pool de garrapatas positivos a este microorganismo (3‚6 % pertenecientes a dos osos palmeros. Los resultados preliminares de este estudio demuestran que Anaplasma spp. podría estar circulante entre garrapatas A. cajennense de animales silvestres en la sabana inundable del municipio de Pore‚ Casanare. Se hace necesario realizar nuevos muestreos para analizar un mayor número de garrapatas y determinar la especie de Anaplasma spp. presente.

  5. Effect of heating systems in litter quality in broiler facilities in winter conditions

    Directory of Open Access Journals (Sweden)

    Ricardo Brauer-Vigoderis

    2014-01-01

    Full Text Available El objetivo de este trabajo, fue evaluar la influencia de los sistemas de calefacción en galpones avícolas, las condiciones psicométricas del aire interno y en la calidad de la cama (humedad y pH, en condiciones de invierno, en el oeste del Estado de Santa Catarina. El experimento fue desarrollado en tres propiedades avícolas integradas en la industria Brasilera Perdigão, en núcleos con tres galpones similares, equipados con diferentes sistemas de calefacción (Campanas infrarrojas a gas, hornos a leña de calentamiento indirecto del aire e intercambiadores de calor por radiación con calentamiento como suplemento de las campanas infrarrojas a gas. Valores de la humedad relativa y la temperatura del aire fueron obtenidos continuamente, en tres puntos de medida de cada instalación al nivel de las aves, para determinar el índice de temperatura y humedad (ITH. Para los análisis de humedad y pH de la cama, fueron colectadas cuatro muestras en cuatro puntos diferentes en cada galpón, cada dos días, durante el periodo en que fueron utilizados los sistemas de calefacción. Los sistemas de calentamiento en campanas infrarrojas a gas y el sistema conjugado de intercambiador más campanas infrarrojas a gas, proporcionaron valores de humedad de la cama, inferiores a los observados en ambientes calentados por el sistema con horno a leña. En los aviarios calentados por los sistemas de campanas a gas y el horno a leña, los valores de ITH, se mantuvieron en una franja de confort para el bienestar de las aves, pero en el sistema conjugado con más campanas a gas, fue detectado estrés y desperdicio de energía. Para todos los sistemas de calentamiento los valores de humedad de la cama se mostraron adecuados, sin embargo, los valores del pH de las muestras de cama, colectadas en los ambientes de cría, evidenciaron un ambiente básico, tornándolo propicio para el crecimiento de bacterias amonificantes.

  6. Revisión taxonómica del género Pimelodella, Eigenmann y Eigenmann, 1888 (pisces, siluriformes: heptapteridae de la región transandina de Colombia

    Directory of Open Access Journals (Sweden)

    Leiva Castaño Mauricio

    2005-07-01

    Full Text Available Las especies de Pimelodella Eigenmann (Heptapteridae, son siluriformes fácilmente identificables por el proceso occipital delgado que se conecta a la placa nucal, aleta adiposa larga y las franjas laterales negras a lo largo del cuerpo. El género se encuentra ampliamente distribuido en la zona tropical de América del sur hasta Costa Rica, muchas de las especies son comunes, pero muy pobremente conocidas. Dentro de los ejemplares revisados de la región transandina de Colombia,
    algunos se encontraron determinados hasta el género y otros mal determinados en la especie. De las siete especies que se encuentran reportadas para esta región (P. chagresi, P. odynea, P. eutaenia, P. grisea, P. modestus, P. reyesi y P. macrocephala se encontró que P. macrocephala no está colectada en los institutos visitados. En los resultados obtenidos se encontraron evidencias morfométricas y merísticas de cuatro grupos diferentes a los grupos reportados, dos hacen parte del sistema del río Magdalena, uno al sistema Caribe–Guajiro y uno al sistema del Pacífico (Colombia.

  7. Efecto de inoculantes microbianos sobre la promoción de crecimiento de plántulas de mangle y plantas de Citrullus vulgaris San Andrés Isla, Colombia

    Directory of Open Access Journals (Sweden)

    Galindo-Castañeda T.

    2005-12-01

    Full Text Available Con el fin de probar la efectividad de dos inoculantes microbianos (producidos a partir de raicillas de mangle rojo y negro en el crecimiento y establecimiento de plántulas de mangle y de patilla, se realizaron cuatro pruebas en campo en San Andrés isla, Colombia. Se aplicaron dichos inoculantes
    en: propágulos de Avicennia germinans colectados en un área de manglar, plántulas de A. germinans y Rhizophora mangle colectadas en cercanía de árboles semilleros, plántulas de A. germinans y R. mangle sembradas y mantenidas en vivero y en semillas de patilla sembradas en un cultivo tradicional libre de fertilizantes químicos. Las variables tomadas como indicativo de crecimiento y desarrollo vegetal fueron: número de nodos, número de hojas y longitud del tallo. Se aislaron selectivamente microorganismos solubilizadores de fosfatos -MSF- y fijadores de nitrógeno -BFNescogiendo los de mayor eficiencia mediante métodos cuantitativos in vitro, para posterior fermentación
    bajo condiciones controladas. Los inoculantes obtenidos se aplicaron en el material vegetal mencionado, efectuando mediciones durante tres meses. Los resultados muestran un efecto positivo de estimulación de crecimiento medido en longitud del tallo en las plantas tratadas con los inoculantes, específicamente en patilla y propágulos de A. germinans mantenidos en vivero.

  8. Caracterización morfológica en árboles nativos de guayaba en el Valle del Cauca

    Directory of Open Access Journals (Sweden)

    Muñoz Jaime E.

    2005-12-01

    Full Text Available Se caracterizaron 53 accesiones de Psidium guajava, colectadas en 9 transectos del Valle del Cauca. En los descriptores cualitativos las accesiones se integraron en tres grupos que se diferenciaron por la forma del fruto. El 75% de los descriptores cuantitativos mostraron CV mayor al 24%. El 72.41% de la variación total de estos descriptores fue explicado en tres CP, que permitieron discriminar para variables de rendimiento del fruto, estructura del árbol y calidad del fruto. Abstract Morphological characteristics of guava in Cauca Valley, Colombia. 53 accesions were collected of Psidium guajava, in 9 transects of the Cauca Valley, these themselves were characterizates. In the descriptors qualitative the accesions were integrated in three groups that were differentiated for the form of the fruit. The 75% of the descriptors quantitative they showed greater VC to 24%. The 72.41% of the total variation of these descriptors, was explained in three PC, that permitted to discriminate for variables of performance of the fruit, structure of the tree and quality of the fruit. Keywords: Psidium guajava, accesions, descriptors quantitative, descriptors qualitative. Principal Component Analysis - PCA

  9. A new species of isopod (Isopoda: Flabellifera: Sphaeromatidae from Cuba, with an identification key for the species of Paraimene Una especie nueva de isópodo (Isopoda: Flabellifera: Sphaeromatidae de Cuba, con una clave de identificación para las especies de Paraimene

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    Manuel Ortiz

    2012-12-01

    Full Text Available A new species of isopod crustacean belonging to the genus Paraimene (Flabellifera, Sphaeromatidae is described. The new species was collected from Cojimar Bay, La Habana Province, Cuba. Paraimene danieli new species differs from all other known species of the genus by the following characters: pereonites 2-3 smooth, pereonites 5-6 carínate, pereonite 7 and pleonite 1 raised at middle point, lacinia mobilis with 3 cusps. An identification key to species of Paraimene is provided.Se describe una especie nueva de isópodo perteneciente al género Paraimene (Isopoda, Flabellifera, Sphaeromatidae. La especie nueva fue colectada de la Bahía de Cojimar, Provincia La Habana, Cuba. Paraimene danieli especie nueva difiere de las especies conocidas del género por los siguientes caracteres: lacinia mobilis con 3 cúspides; pereonites 2-3 lisos, pereonites 5-6 carinados, pereonite 7 y pleonite 1 elevados medialmente. Se presenta una clave de identificación para las especies de Paraimene.

  10. A new species of Eccopsis Zeller (Lepidoptera, Tortricidae from the coastal valleys of northern Chile, with the first continental record of E. galapagana Razowski & Landry

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    Héctor A. Vargas

    2011-06-01

    Full Text Available A new species of Eccopsis Zeller (Lepidoptera, Tortricidae from the coastal valleys of northern Chile, with the first continental record of E. galapagana Razowski & Landry. Eccopsis Zeller, 1852 is reported for the first time from Chile. Eccopsis razowskii Vargas, n. sp. is described and illustrated based on specimens reared from larvae collected on native Acacia macracantha Willd. (Fabaceae in the coastal valleys of the northern Chilean desert. Eccopsis galapagana Razowski & Landry, 2008, previously known only from the Galapagos Islands, Ecuador, is recorded for the first time from continental South America. Larvae of the latter were collected in northern Chile feeding on Prosopis alba Griseb (Fabaceae.Uma nova espécie de Eccopsis Zeller (Lepidoptera, Tortricidae dos valles litorais do norte do Chile, e o primeiro registro continental de E. galapagana Razowski & Landry. Eccopsis Zeller (Lepidoptera, Tortricidae é registrado pela primeira vez para o Chile. Eccopsis razowskii Vargas, n. sp. é descrita e ilustrada com base em espécimes criados de larvas colectadas em Acacia macracantha Willd. (Fabaceae nos vales litorais do deserto do norte do Chile. Eccopsis galapagana Razowski & Landry, 2008, conhecida previamente das Ilhas Galápados, Equador, é registrada pela primeira vez para SulAmérica continental. Suas larvas foram coletadas em Prosopis alba Griseb (Fabaceae.

  11. ESTUDIO BIOGEOQUÍMICO DEL EMBALSE SUATA, VENEZUELA, DE ACUERDO AL CONTENIDO METÁLICO DE SUS SEDIMENTOS DE FONDO, AGUAS Y PECES

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    María A. Álvarez

    2012-01-01

    Full Text Available Se realizó un estudio biogeoquímico mediante el análisis del contenido metálico en muestras de sedimentos de fondo, aguas y peces colectadas mensualmente durante un año. Los sedimentos se encontraron enriquecidos antrópicamente con COT, Zn y Cd, presentando un nivel de contaminación de moderado a fuerte, y según la fracción biodisponible, un riesgo muy alto de Zn y mediano de Ni, Cd, Cr y Co. Estas especies llegarían al embalse, principalmente, mediante la escorrentía superficial de desechos de industrias de producción animal ubicadas en los alrededores y aportes del Rio Aragua. Aunque la desorción de los metales desde los sedimentos es improbable dado el pH donde se encuentran (8-9 y la concentración de los metales en las aguas y peces fue menor que las máximas permitidas en las normativas, se encontraron indicios de bioacumulación de Hg y Ni en el hígado de los peces que sugieren mantener el monitoreo del embalse.

  12. Protozoarios parásitos de importancia en salud pública transportados por Musca domestica Linnaeus en Lima, Perú

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    Martín Cárdenas

    2013-06-01

    Full Text Available El presente trabajo investiga los protozoarios parásitos de importancia para la salud pública transportados por Musca domestica. El estudio se llevó a cabo entre los meses de junio y diciembre de 1998, en los distritos de Comas y San Juan de Lurigancho de la provincia de Lima. Se estudiaron 3014 moscas colectadas en la basura acumulada en la vía pública de los pueblos jóvenes de los distritos mencionados. Las capturas se agruparon en lotes constituidos por un promedio de 30 moscas. Se estudiaron un total de 100 lotes. Las moscas fueron disecadas y procesadas con agua destilada estéril para obtener un macerado intestinal. Para la observación de los parásitos se utilizó el método directo simple y la coloración tricrómica de Gomori ácido resistente modificado. Se encontraron los siguientes protozoarios que parasitan al hombre: Blastocystis hominis (9,0%, Giardia lamblia (3,0%, Cryptosporidium sp. (2,0%, Cyclospora cayetanensis (1,0%, Iodamoeba bütschlii (17,0%, Endolimax nana (5,0% y Chilomastix mesnili (3,0%. Se reporta por primera vez para el Perú a B. hominis y Cyclospora cayetanensis en M. domestica.

  13. Distribución geográfica de Boeckella y Neoboeckella (Calanoida: Centropagidae en el Perú

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    Iris Samanez

    2014-12-01

    Full Text Available El análisis de muestras de plancton colectadas en diferentes localidades a lo largo de los Andes peruanos, dieron como resultado el registro de siete especies de Boeckella (gracilis, gracilipes, calcaris, poopoensis, occidentalis, titicacae y palustris y dos de Neoboeckella (kinzeli y loffleri. Todas las especies citadas, exceptuando a las especies de Neoboeckella, fueron registradas en la cuenca del lago Titicaca (Puno. Además, B. palustris, B. gracilipes y B. calcaris fueron también reportadas en Moquegua, Apurímac y Pasco (Andes del sur y central. Boeckella titicacae parece estar restringida a la cuenca del lago Titicaca. Boeckella poopoensis ocurre en cuerpos de agua con elevada conductividad reportándose sólo en Las Salinas en Arequipa. Boeckella occidentalis fue la especie con mayor rango de distribución desde el sur en Puno hasta el norte en Cajamarca y se registra por primera vez para el país Neoboeckella loffleri. Las muestras están depositadas en la Colección de Plancton del Departamento de Limnología del Museo de Historia Natural de la Universidad Nacional Mayor de San Marcos, Lima-Perú.

  14. El desarrollo temprano del calamar patagónico Loligo gahi D’Orbigny,1835 en aguas peruanas (Cephalopoda: Loliginidae

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    Franz Cardoso

    2013-05-01

    Full Text Available El desarrollo temprano del calamar patagónico, Loligo gahi D’Orbigny, 1835 fue estudiado en el campo y en el laboratorio. Las puestas colectadas en la Isla San Lorenzo, Perú, fueron transferidas al laboratorio, e incubadas en un sistema cerrado de agua marina. Las cápsulas midieron de 88 a 169 mm de longitud y cada cápsula contenía entre 56 y 114 huevos fertilizados. Los huevos midieron de 1,7 a 2,1 mm de longitud y la longitud del manto de los individuos eclosionados varió de 1,9 a 2,8 mm. El desarrollo de las paralarvas se logró a los 20 días, a una temperatura promedio de 19 ºC. El patrón de desarrollo embrionario es similar al observado en otras especies de Loligo. Las paralarvas sobrevivieron 45 días con una dieta de zooplancton (copepódos, micidáceos y larvas de poliquetos.

  15. Separación e identificación de algunas toxinas del veneno de Centruroides margaritatus (Gervais,1841 (Scorpiones : Buthidae

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    Enrique Escobar

    2013-06-01

    Full Text Available Las proteínas del veneno del escorpión Centruroides margaritatus, fueron separadas mediante cromatografía de intercambio catiónico en CM-Sephadex C-25 con buffer acetato de amonio 0,05M a pH 7, a partir de 28,3 mg de veneno obtenidos de 52 ejemplares adultos capturados en la provincia de Zarumilla, Tumbes, norte del Perú. El perfil cromatográfico mostró la presencia de 9 picos de proteína y los ensayos de toxicidad han permitido identificar tres tipos de toxinas, cada una específica para crustáceos, insectos y roedores, respectivamente. Tanto en el veneno crudo como en las fracciones colectadas, no se encontró actividad de fosfolipasa ni actividad proteolítica. La PAGE-SDS del veneno crudo, muestra la presencia de una banda bastante notoria de aproximadamente 14 KDa, y otras dos muy tenues, de aproximadamente 45KDa, lo cual significa que la mayoría de proteínas de este veneno son de peso molecular igual o menor a 14KDa.

  16. Lista de esponjas marinas asociadas al arrecife Tuxpan, Veracruz, México Checklist of marine sponges from Tuxpan Reef, Veracruz, Mexico

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    Carlos González-Gándara

    2009-04-01

    Full Text Available Se presenta la lista de esponjas marinas (Porifera: Demospongiae del arrecife Tuxpan, Veracruz, México, colectadas en 2004, 2005 y 2006 mediante buceo libre y con equipo autónomo SCUBA. Los resultados muestran la presencia de 18 especies pertenecientes a 13 géneros y 13 familias, 17 de estas especies son nuevos registros para los arrecifes coralinos del norte de Veracruz y una (Aplysina cauliformis Carter, 1882 para el estado. La información puede auxiliar para definir las estrategias de manejo, monitoreo y protección de estas formaciones arrecifales que recientemente han sido propuestas como área de protección de flora y fauna.A checklist of marine sponge species (Porifera: Demospongiae from Tuxpan reef, Veracruz, Mexico, collected during 2004, 2005 and 2006 by free and SCUBA diving equipment, is presented. The results show the presence of 18 species belonging to 13 genera and 13 families. 17 speices represent new records for the northern coral reefs of Veracruz, and the 18th species (Aplysina cauliformis Carter, 1882 is a new record for the state. This information may help to define appropriate management, monitoring and protection strategies for the coral reefs of the north of Veracruz, which have been proposed as a natural preserve area recently.

  17. Caracterización bioquímica y fisiológica de algunos frutos amazónicos (Capsicum sp. Y Eugenia stipitata MC VAUGH

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    Hernández María Soledad

    2006-06-01

    Full Text Available Los niveles de la actividad poligalacturonasa (PG fueron cuantificados en diferentes estadios de madurez de frutos de cuatro especies de ají Capsicum chinense, Capsicum baccatum, Capsicum annuum y Capsicum frutescens. Las muestras fueron colectadas y almacenadas a -10º C. La determinación de la actividad poligalacturonasa fue medida por el método de azúcares reductores de Somogyi-Nelson y el contenido de proteínas por Bradford. El comportamiento de la actividad PG de la especie C. chinense es muy temprano y similar a C. frutescens, pero el rango de la actividad PG de C. chinense es inferior a la de C. frutescens (picos máximos de 1678,572 y
    3581,5080 nmoles*min-1*mg prot-1, respectivamente. C. frutescens presentó dos períodos de actividad; y al igual que C. annuum, C. baccatum es de actividad tardía. El pico climatérico del fruto de Arazá fue de 102,49 mgCO2/kg/h, mientras que el pico de etileno fue de 26,62 mgC2H4/kg/h coincidiendo con la máxima madurez del fruto.

  18. ESTIMACIÓN DE LA SUPERFICIE FOLIAR EN DOS VARIEDADES DE PAPA (Solanum tuberosum L. POR MÉTODOS NO DESTRUCTIVOS

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    Eduardo Jerez Mompie

    2014-01-01

    Full Text Available El trabajo se desarrolló en el Instituto Nacional de Ciencias Agrícolas con el objetivo de determinar una función matemática a partir de las medidas lineales de las hojas de papa para estimar la superficie foliar de la planta de manera no destructiva. Se utilizaron plantas de dos variedades de papa Call White y Santana colectadas a los 40, 60 y 70 días después de la plantación, para asegurar que existieran hojas de distintos tamaños. Una vez realizadas las medidas lineales de las hojas (largo y ancho se procedió a determinar la superficie foliar real de la hoja con el empleo de un integrador de superficie foliar AM300 y a partir de estas variables se establecieron las regresiones lineales correspondientes al área real de cada hoja con cada una de las longitudes obtenidas y el producto de ellas. Se tuvo en cuenta el resultado del coeficiente de correlación y de determinación para escoger la función lineal que con mayor exactitud estima la superficie foliar de la hoja. La ecuación obtenida a partir del producto del largo por el ancho resultó la de mayor coeficiente de determinación en ambas variedades.

  19. ESTUDIO DE LA BIODIVERSIDAD EN HUERTOS AGRÍCOLAS URBANOS DE DOS MUNICIPIOS DE CIUDAD DE LA HABANA. CARACTERIZACIÓN DE LAS ACCESIONES DEL GÉNERO VIGNA

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    R. Ortiz

    2001-01-01

    Full Text Available Se realizó un estudio de la biodiversidad agrícola en los huertos urbanos de ocho localidades en los municipios Boyeros y Arroyo Naranjo, con el objetivo de inventariar los cultivos agrícolas existentes. Entre los meses de abril a junio de 1997 se realizó la visita a 100 huertos, llevándose el registro de los datos pasaporte a través del formato que existe a nivel internacional, el cual fue ajustado a las condiciones de este trabajo. Finalmente en un género hortícola colectado fue eva- luada la biodiversidad encontrada en superficies del ISPETP durante los meses de abril a julio de 1998. La información obte- nida se analizó estadísticamente por métodos univariados y multivariados. Se determinó que en los huertos estudiados existe una biodiversidad agrícola satisfactoria, localizándose 262 accesiones entre las que predominan las viandas, frutales, hortalizas y leguminosas. Además, muchos de los cultivares se adaptan a sistemas de agricultura de bajos insumos. En los tipos de habichuelas colectadas se encontró diversidad para una gran cantidad de caracteres morfológicos y componentes del rendimiento.

  20. Epidemiología de la colección, proceso y uso de sangre y componentes sanguíneos en el Valle del Cauca, Colombia.

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    Armando Cortés B.

    2009-10-01

    Full Text Available Para evaluar la práctica transfusional y los efectos del suministro de sangre, se estudió la colección y transfusión de sangre en el Valle del Cauca, Colombia, mediante una encuesta de información con las secretarías de Salud Municipal de Cali y Departamental, y los resultados de una muestra de hospitales y clínicas del departamento. Se usaron métodos estadísticos para calcular las actividades principales. El cuestionario incluyó información del donante de sangre, número de unidades colectadas y procedimiento de colección por entidad, servicios ofrecidos, producción y uso de componentes sanguíneos durante 1997. Para calcular la tasa de incidencia de transfusiones de sangre y componentes en la población general y en edad y grupos específicos, se tomó una muestra de habitantes del departamento del Valle que recibieron transfusiones durante 1997. El estudio determinó los casos de transfusión en un grupo de individuos bien definido y en un tiempo específico. Este estudio proporciona datos recientes en las actividades de donación y transfusión que son vitales para evaluar la práctica transfusional. Es el primer informe de tasa de incidencia de transfusión sanguínea en Colombia.

  1. Primer hallazgo de Aedes albopictus Skuse en el Gran Área Metropolitana de Costa Rica

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    Rodrigo Marín Rodríguez

    2014-06-01

    Full Text Available Objetivo: Informar sobre el primer hallazgo de Aedes albopictus en el Gran Área Metropolitana de Costa Rica. Métodos: Formas inmaduras de mosquitos colectadas en la Ciudadela La Carpio, Distrito Uruca, San José, como parte de una barrida epidemiológica fueron diagnosticadas preliminarmente como Ae. albopictus. Dicho material se fijó en alcohol al 70 %, se aclaró en lactofenol y se evaluó taxonómicamente mediante claves dicotómicas especializadas. Resultados: Las formas inmaduras evaluadas procedieron de dos criaderos, un tarro y un balde. Las características de las larvas y pupas permitieron identificarlas taxonómicamente como Ae. albopictus. Este es el primer reporte sobre la presencia de dicho vector en Costa Rica. Discusión: La presencia de Ae. albopictus en la Gran Área Metropolitana ocurre como parte del proceso de expansión que está teniendo dicho vector por el territorio nacional. Su presencia debe ser motivo de estudio para determinar su eventual papel en la transmisión del dengue. Por otro lado la presencia de este vector complica la gestión de la vigilancia entomológica de dengue que ha estado dirigida fundamentalmente a la identificación y control del Aedes aegypti.

  2. Estudio preliminar de leptospirosis en roedores y canes en salitral, Piura-1999

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    Rosa Sacsaquispe C

    2003-03-01

    Full Text Available Objetivo: Determinar serológica y bacteriológicamente la presencia de Leptospira en muestras de suero y riñón de carnes y roedores, del distrito de Salitral, departamento de Piura (norte del Perú.Materiales y métodos: Estudio realizado en octubre de 1999. Se capturaron roedoresutilizando trampas tomahawk en las localidades de Salitral y Malacasi. Se utilizó la prueba de aglutinación microscópica (MAT para la detección de anticuerpos y el cultivo de tejido de riñón para el estudio bacteriológico. Asimismo, se evaluaron muestras de suero de canes de la localidad del Salitral.Resultados: 2 de 12 roedores identificados como Rattus rattus (16,6% reaccionaron con Leptospira serovar grypotyphosa a un título de 1/200 y 1/400, en tanto que una de las 3 muestras de suero de can colectadas reaccionó con Leptospira serovar canicola. De los 12 cultivos de las muestras de riñón de roedores ninguno fue positivo a Leptospira. Conclusiones: La detección de anticuerpos contra Leptospira en el distrito de Salitral sugiere ampliar los estudios de Leptospira en la zona.

  3. DISTRIBUCIÓN DE DOS INDICADORES BACTERIANOS DE CALIDAD DE AGUA EN EL GOLFO DE URABÁ, CARIBE COLOMBIANO

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    GÓMEZ VELÁSQUEZ. FELIPE ANDRÉS

    2010-05-01

    Full Text Available Entre el 13 de Junio de 2006 y el 16 de Junio de 2007, las bacterias indicadoras de calidad ambiental y sanitaria, Coliformes Totales (CT y Coliformes Fecales (CF, fueron determinadas en las aguas del Golfo de Urabá, Colombia, empleando el método del NMP, mediante la técnica de tubos múltiples. El estudio fue realizado para evaluar la distribución de estos indicadores microbianos a nivel vertical y horizontal y la relación con algunas variables fisicoquímicas de temperatura del agua, salinidad, oxígeno disuelto y pH; y para así poder definir la relevancia de estos indicadores en ecosistemas marinos. La mayoría de las muestras colectadas presentó concentraciones de los indicadores menores o iguales a la norma. Las áreas donde se determinó la mayor concentración de bacterias coniformes, correspondieron a los sitios ubicados cerca de la desembocadura de los ríos Atrato y León, donde las concentraciones salinas fueron bajas. El análisis estadístico muestra la salinidad como el principal factor ambiental responsable de la concentración y distribución de las bacterias CT y CF para las estaciones evaluadas en el Golfo de Urabá, evidenciando un efecto inhibidor sobre estos dos indicadores.

  4. DESCRIPCIÓN MACROSCÓPICA DEL DESARROLLO LARVAL DEL COPORO (PROCHILODUS MARIAE

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    N. López

    2005-01-01

    Full Text Available El presente estudio fue realizado en la estación piscícola La Terraza, unidad perteneciente al Instituto Colombiano de Desarrollo Rural (INCODER y adscrita a la Facultad de Medicina Veterinaria y de Zootecnia de la Universidad Nacional de Colombia, localizada en la ciudad de Villavicencio (Meta. Fueron utilizadas larvas de coporo (Prochilodus mariae, obtenidas por reproducción inducida, que fueron colectadas desde la eclosión y cada 6 horas hasta la reabsorción del saco vitelino. Con la ayuda de una balanza de precisión y un estereoscopio con ocular micrométrico se registraron el peso, la longitud total y la longitud y altura del saco vitelino. Los resultados mostraron que las larvas midieron 3,11±0,42 mm y pesaron 0,43±0,15 mg en el momento de la eclosión y 5,94±0,23 mm y 0,95±0,13 mg al final del estudio. El volumen del saco vitelino fue de 0,655 mm3 en la eclosión y de 0,0074 mm3 a las 74 horas posteclosión (HPE. Las larvas completaron su desarrolloa las 84 HPE, presentaron abertura completa de la boca, llenado de la vejiga gaseosa y natación horizontal.

  5. DESCRIPCIÓN MACROSCÓPICA DEL DESARROLLO LARVAL DEL COPORO (Prochilodus mariae

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    Landines M.

    2005-11-01

    Full Text Available El presente estudio fue realizado en la estación piscícola La Terraza, unidad perteneciente al Instituto Colombiano de Desarrollo Rural (INCODER y adscrita a la Facultad de Medicina Veterinaria y de Zootecnia de la Universidad Nacional de Colombia, localizada en la ciudad de Villavicencio (Meta. Fueron utilizadas larvas de coporo (Prochilodus mariae, obteni¬das por reproducción inducida, que fueron colectadas desde la eclosión y cada 6 horas hasta la reabsorción del saco vitelino. Con la ayuda de una balanza de precisión y un estereoscopio con ocular micrométrico se registraron el peso, la longitud total y la longitud y altura del saco vitelino. Los resultados mostraron que las larvas midieron 3,11±0,42 mm y pesaron 0,43±0,15 mg en el momento de la eclosión y 5,94±0,23 mm y 0,95±0,13 mg al final del es¬tudio. El volumen del saco vitelino fue de 0,655 mm3 en la eclosión y de 0,0074 mm3 a las 74 horas posteclosión (HPE. Las larvas completaron su desarrollo a las 84 HPE, presentaron abertura completa de la boca, llenado de la vejiga gaseosa y natación horizontal.

  6. Sinopsis de las hormigas dacetinas de los géneros daceton perty y acanthognathus mayr (formicidae: myrmicinae de Colombia

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    Fernández Fernando

    2007-04-01

    Full Text Available Los géneros Acanthognathus y Daceton son dos géneros de distribución neotropical que pertenecen a la tribu
    Dacetini (Formicidae: Myrmicinae. Esta tribu está compuesta por nueve géneros de los cuales cuatro son
    de distribución neotropical (Acanthognathus, Daceton, Pyramica y Strumigenys. Acanthognathus y Daceton son endémicos de esta región. Las especies reportadas para Colombia del género Acanthognathus eran dos (A.
    teledectus, A. ocellatus. Daceton es un género monotípico (D. armigerum reportado para Colombia. Se revisaron
    dos colecciones (IAvH, ICN, en las cuales se encontraban especies de estos dos géneros. Como resultado
    se obtuvo una nueva especie registrada para Colombia del género Acanthognathus (A. brevicornis y una nueva especie para la ciencia. En cuanto al género Daceton, este se encontró distribuido a varios departamentos de la Orinoquía y Amazonía colombianas (Caquetá, Meta, Guaviare, Vichada, Guanía. El uso intensivo de trampas especializadas (principalmente Winkler así como el continuo muestreo en áreas protegidas o de interés ha incrementado el número de especies de hormigas de hojarasca colectadas, entre las cuales se encuentran las de estos dos géneros.

  7. Estudio bioquímico del veneno de Tityus kaderkai(Scorpiones: Buthidae con notas sobre su distribución y hábitat en el Perú

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    Enrique Escobar

    2013-12-01

    Full Text Available Se ha estudiado bioquímicamente el veneno del escorpión Tityus kaderkai Kovařik, 2005 del departamento de Madre de Dios. El veneno soluble contiene 47.6% de proteína y por PAGESDS muestra cinco bandas proteicas. Las proteínas del veneno fueron separadas, a partir de 12.9 mg de veneno, mediante cromatografía de intercambio catiónico en CM Sephadex C-25 con buffer acetato de amonio 0.05 M pH 7, obteniéndose 7 picos proteicos (I – VII. Los ensayos de toxicidad han permitido identificar tres toxinas que afectan a Mus musculus y que se encuentran asociadas a los picos IV, V y VII; asimismo, se ha detectado toxicidad sobre Gryllus sp. en los picos IV, V, VI y VII. Entre las actividades enzimáticas ensayadas, se ha encontrado actividad proteolítica sobre caseína en el pico I y actividad de hialuronidasa en el pico IV con una actividad específica de 205.6 μg/min/mg. Tanto en el veneno soluble como en las fracciones colectadas no se encontró actividad de fosfolipasa, anticoagulante ni hemolítica. El trabajo incluye notas sobre la distribución y el hábitat de la especie.

  8. Especies de hongos formadores de Micorrizas Arbusculares: nuevas citas para la República Argentina Arbuscular mycorrhizal fungal species: new records for Argentina

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    G. Irrazabal

    2005-07-01

    Full Text Available Se investigó la presencia de hongos formadores de micorrizas arbusculares asociados a plantas colectadas en un agroecosistema y en un bosque nativo de tala, en la provincia de Buenos Aires. Las especies que constituyen un nuevo registro para la Argentina son: Scutellospora dipapillosa (Walker & Koske Walker & Sanders y Scutellospora fulgida Koske & Walker halladas en la rizósfera de Triticum aestivum L.; Scutellospora gilmorei (Trappe & Gerd. Walker & Sanders, en la rizósfera de Celtis tala Gill. Ex Planch., Scutia buxifolia Reiss. y plantas herbáceas; Acaulospora delicata Walker, Pfeiffer & Bloss. y Glomus clarum Nicolson & Schenck, halladas en ambos sitios. Estas especies son citadas, descriptas e ilustradas por primera vez para Argentina. Glomus etunicatum Becker & Gerdemann ha sido citada pero no descripta e ilustrada para nuestro país.The presence of arbuscular mycorrhizal fungi associated with plants from an agroecosystem and a native forest, in Buenos Aires province is studied. The species that correspond a new register for Argentina are: Scutellospora dipapillosa (Walker & Koske Walker & Sanders and Scutellospora fulgida Koske & Walker found in the rhizosphere of Triticum aestivum L.; Scutellospora gilmorei (Trappe & Gerd. Walker & Sanders, in the rhizosphere of Celtis tala Gill. Ex Planch., Scutia buxifolia Reiss. and herbaceous plants; Acaulospora delicata Walker, Pfeiffer & Bloss. and Glomus clarum Nicolson & Schenck, occurred in both sites. They are cited, described and illustrated for the first time from Argentina. Glomus etunicatum Becker & Gerdemann was previously cited although it was not described and illustrated from Argentina.

  9. REVISIÓN DE ESTOMATITIS ULCERATIVA EN OFIDIOS

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    S M Peñuela

    2007-01-01

    Full Text Available La estomatitis en ofidios es una condición de frecuente diagnóstico en la clínica de animales silvestres.Se relaciona principalmente con eventos estresantes como deficiencias medioambientales,nutricionales o una enfermedad subyacente, las cuales terminan favoreciendo el crecimientode gérmenes patógenos y, de esta forma, llevan a la muerte del animal. Los signos clínicos puedenpasar inadvertido s por un tiempo variable, por lo que se recomienda hacer revisiones periódicas alos ofidios en cautiverio. Debido a que es una entidad multifactorial, uno o varios agentes etiológicosse implican como causales y perpetuantes de la condición, por lo que es de gran importanciasometer las muestras colectadas a análisis específicos, para así poder identificarlos y garantizarun mayor éxito en el momento de instaurar un tratamiento. En casos avanzados el pronóstico casisiempre es malo debido a la posibilidad de una diseminación hematógena, con sus efectos secundarioscomo neumonía y osteomielitis, o por continuidad, causando gastroenteritis. Por ello, laprincipal herramienta de combate frente a la entidad es minimizar la exposición de los ofidios afactores predisponentes o estresantes, que sin lugar a dudas ponen en peligro la vida del ani;nal.

  10. Viabilidad y germinación de semillas de tres especies arbóreas nativas de la selva tropical, Chiapas, México

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    Carolina Orantes-García

    2015-08-01

    Full Text Available Se determinó la proporción de semillas viables y la pérdida de viabilidad debido al periodo de almacenamiento, así como el efecto de tratamientos pregerminativos que favorecen la germinación en semillas deCordia alliodora, Terminalia amazoniayBursera bipinnata, árboles nativos de la selva tropical, Chiapas, México. Se encontró que las semillas recién colectadas presentaron más del 90% de viabilidad, la cual fue disminuyendo hasta mostrar un 15% enB. bipinnata, 34% enC. alliodoray 18% enT. amazoniadespués de 12 meses de almacenamiento. De acuerdo con la germinación acumulada se encontraron diferencias significativas entre los tratamientos (pB. bipinnata63%,C. alliodora62% yT. amazonia54%. En conclusión, para poder obtener un mayor porcentaje de semillas germinadas en poco tiempo, es necesario dar un tratamiento previo a la siembra para las semillas de estas especies. El almacenamiento de las semillas durante un año provocó una declinación en la capacidad germinativa.

  11. Detección de anticuerpos contra Borrelia burgdorferi e identificación de garrapatas ixodidas en Piura Y Amazonas, Perú

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    Martha Glenny A

    2004-01-01

    Full Text Available Objetivos: Detectar anticuerpos IgG/IgM contra Borrelia burgdorferi en población general, procedentes de los departamentos de Piura y Amazonas e identificar especies de garrapatas probablemente incriminadas en la transmisión de la enfermedad de Lyme. Material y Métodos: Entre agosto del año 2001 y junio de 2002, se colectaron muestras de sangre de 232 pobladores procedentes de ocho localidades del Departamento de Piura y 12 del Departamento de Amazonas, para evaluar mediante ELISA Captia™ Lyme IgG/IgM (Trinity biotech la presencia de anticuerpos contra Borrelia burgdorferi. Además, se colectaron garrapatas en animales domésticos por búsqueda directa. Resultados: Se detectó seropositividad en 9,9 % de los sueros evaluados. Asimismo, de 433 garrapatas colectadas se identificaron los géneros: Ixodes (5,5%, Amblyomma (18,0%, Rhipicephalus (23,5%, Anocentor (31,1% y Boophilus (21,7%. Conclusiones: Existen personas seropositivas por Borrelia en Piura y Amazonas, coincidiendo con los hallazgos realizados en Sapillica en el año 1992, además se detectó la presencia de garrapatas del género Ixodes en Piura.

  12. MAMÍFEROS PEQUEÑOS EN LA DIETA DE LA LECHUZA TYTO ALBA (STRIGIFORMES: TYTONIDAE EN DOS LOCALIDADES DEL OCCIDENTE DE ECUADOR, CON AMPLIACIÓN DISTRIBUCIONAL DE ICHTHYOMYS HYDROBATES (RODENTIA: CRICETIDAE

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    JORGE BRITO M.

    2015-01-01

    Full Text Available En el presente estudio a partir de 107 egagrópilas de la lechuza, determinamos el consumo de 300 presas agrupadas en 21 especies, las egagrópilas fueron colectadas en dos localidades de diferente ambiente Los Santiagos y La Ciénaga, en las provincias de Chimborazo y Manabí, al occidente de Ecuador. Nuestros análisis revelaron una dieta compuesta mayoritariamente de mamíferos pequeños, sobre todo roedores, quienes constituyeron el 80%. Entre las presas más abundantes se encontró a Oligoryzomys sp. que representó el 38.7% de la dieta en las muestras de Los Santiagos y Sigmodon peruanus 33.6% en La Ciénaga, siendo el 22,5% y 41% respectivamente de la biomasa total consumida para cada sitio. La rata cangrejera Ichthyomys hydrobates es reportada por primera vez en la dieta de la lechuza y también en la localidad de Los Santiagos, ampliando el rango distribucional de este roedor para el centro-sur al occidente de Ecuador en aproximadamente 200 km.

  13. Anopheles larval abundance and diversity in three rice agro-village complexes Mwea irrigation scheme, central Kenya

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    Mwangangi Joseph M

    2010-08-01

    Full Text Available Abstract Background The diversity and abundance of Anopheles larvae has significant influence on the resulting adult mosquito population and hence the dynamics of malaria transmission. Studies were conducted to examine larval habitat dynamics and ecological factors affecting survivorship of aquatic stages of malaria vectors in three agro-ecological settings in Mwea, Kenya. Methods Three villages were selected based on rice husbandry and water management practices. Aquatic habitats in the 3 villages representing planned rice cultivation (Mbui Njeru, unplanned rice cultivation (Kiamachiri and non-irrigated (Murinduko agro-ecosystems were sampled every 2 weeks to generate stage-specific estimates of mosquito larval densities, relative abundance and diversity. Records of distance to the nearest homestead, vegetation coverage, surface debris, turbidity, habitat stability, habitat type, rice growth stage, number of rice tillers and percent Azolla cover were taken for each habitat. Results Captures of early, late instars and pupae accounted for 78.2%, 10.9% and 10.8% of the total Anopheles immatures sampled (n = 29,252, respectively. There were significant differences in larval abundance between 3 agro-ecosystems. The village with 'planned' rice cultivation had relatively lower Anopheles larval densities compared to the villages where 'unplanned' or non-irrigated. Similarly, species composition and richness was higher in the two villages with either 'unplanned' or limited rice cultivation, an indication of the importance of land use patterns on diversity of larval habitat types. Rice fields and associated canals were the most productive habitat types while water pools and puddles were important for short periods during the rainy season. Multiple logistic regression analysis showed that presence of other invertebrates, percentage Azolla cover, distance to nearest homestead, depth and water turbidity were the best predictors for Anopheles mosquito larval

  14. 水生植物的生态敏感度研究%A Study on Ecological Sensitivities of Several Plants

    Institute of Scientific and Technical Information of China (English)

    李宏文; Chien,PaulK

    2001-01-01

    By measuring ecological effects of several aquatic plants on heavy metals of Pb and Zn,it has been shown that the ecological sensitivities of plants on danger environmental factors are quite different between organism level and molecular level.According to CAT activity,an order for CAT- sensitivities of plants was found to be:Alternanthera philoxeroides>Spirodella polyrrhiza>Azolla imbricata>Hydrilla verticillata>Potamogeton malaianus.This result was contrary to the species- sensitivities based on injured levels of plants,such as:Potamogeton malaianus>Hydrilla verticillata>Azolla imbricata>Spirodella polyrrhiza>Alternanthera philoxeroides.The ecological sensitivity introduced into ecological planning has been recognized as index for delimiting ecological sensitive areas.The different idea of CAT- sensitivity and species- sensitivity may be applied to different areas with different ecological stabilities and different protecting needs.%通过人工室外玻璃水槽养殖试验,研究了几种水生植物在两个不同的生态水平上对重金属 Pb和 Zn的生态效应。结果发现,水生植物对有害环境因子的生态敏感度,在植物组织水平上与在分子水平上的表现截然相反。根据植物体内过氧化氢酶( CAT)的活性反应来排序,几种水生植物的( CAT)敏感度为:水花生 >紫背萍 >满江红 >轮叶黑藻 >马来眼子菜。这与植物受 Pb和 Zn伤害后的形态症状表现的生态敏感度(种敏感度)顺序完全相反。把不同的生态敏感度概念( CAT敏感度和种敏感度)引入生态规划,作为生态敏感区域划定的指标。对具有不同敏感等级和不同的保护水平要求的生态区域,应采用不同的生态敏感度,即 CAT敏感度或种敏感度。

  15. Reconstruction of the Eocene Arctic Ocean Using Ichthyolith Isotope Analyses

    Science.gov (United States)

    Gleason, J. D.; Thomas, D. J.; Moore, T. C.; Waddell, L. M.; Blum, J. D.; Haley, B. A.

    2007-12-01

    Nd, Sr, O and C isotopic compositions of Eocene fish debris (teeth, bones, scales), and their reduced organic coatings, have been used to reconstruct water mass composition, water column structure, surface productivity and salinities of the Arctic Ocean Basin at Lomonosov Ridge between 55 and 44 Ma. Cleaned ichthyolith samples from IODP Expedition 302 (ACEX) record epsilon Nd values that range from -5.7 to -7.8, distinct from modern Arctic Intermediate Water (-10.5) and North Atlantic Deep Water. These Nd values may record some exchange with Pacific/Tethyan water masses, but inputs from local continental sources are more likely. Sr isotopic values are consistent with a brackish-to-fresh water surface layer (87Sr/86Sr = 0.7079-0.7087) that was poorly mixed with Eocene global seawater (0.7077-0.7078). Leaching experiments show reduced organic coatings to be more radiogenic (>0.7090) than cleaned ichthyolith phosphate. Ichthyolith Sr isotopic variations likely reflect changes in localized river input as a function of shifts in the Arctic hydrologic cycle, and 87Sr/86Sr values might be used as a proxy for surface water salinity. Model mixing calculations indicate salinities of 5 to 20 per mil, lower than estimates based on O isotopes from fish bone carbonate (16 to 26 per mil). Significant salinity drops (i.e., 55 Ma PETM and 48.5 Ma Azolla event) registered in oxygen isotopes do not show large excursions in the 87Sr/86Sr data. Carbon isotopes in fish debris record a spike in organic activity at 48.5 Ma (Azolla event), and otherwise high-productivity waters between 55 and 44 Ma. The combined Sr-Nd-O-C isotopic record is consistent with highly restricted basin-wide circulation in the Eocene, indicative of a highly stratified water column with anoxic bottom waters, a "fresh" water upper layer, and enhanced continental runoff during warm intervals until the first appearance of ice rafted debris at 45 Ma.

  16. Duração do ciclo de vida de Cornops aquaticum (Bruner, 1906 (Orthoptera: Acrididae: Leptysminae e aspectos de seu comportamento alimentar na Amazônia central Life cycle of Cornops aquaticum (Bruner, 1906 (Orthoptera: Acrididae: Leptysminae and aspects of its food behavior at Central Amazonia

    Directory of Open Access Journals (Sweden)

    Maria de Fátima Vieira

    2003-12-01

    Full Text Available O gafanhoto Cornops aquaticum (Bruner, 1906 (Orthoptera: Acrididae: Leptysminae completa seu ciclo de vida em Eichhornia (Pontederiaceae. Em experimentos ao ar livre, durante o período da enchente, foi verificado que a cópula pode durar entre 40 a 120 minutos. Cada postura tem em média 13,4 ovos (+/- 3,2 e o período de incubação durou 34,1 dias (+/- 1,97. A duração média do desenvolvimento de ovo a adulto foi de 156,2 dias, sendo 34,1 do estágio de ovo, 53,4 do estágio ninfal e 68,7 do estágio adulto. Alimentou-se de 6 espécies de macrófitas aquáticas dentre as 13 oferecidas. Ninfas (N e Adultos (A sobreviveram mais dias em Pistia stratiotes (N = 43 % até 30 dias e A =1 % até 27 dias e Pontederia sp. (N = 85 % e A = 40 % até 24 dias que em Limnobium sp. (N =13% e A =10 % até 12 dias, Ludwigia sp. (N =3 % até 24 dias e A =15 % até 9 dias, Azolla sp. (N =35 % até 9 dias e A =15 % até 12 dias e Paspalum repens (N =3 % até 21 dias e A =5 % até 24 dias.Life cycle of the grasshopper Cornops aquaticum (Bruner, 1906 (Orthoptera: Acrididae: Leptysminae is dependent on Eichhornia (Pontederiaceae. Experiments were carried outdoors during receding water, the Mating last for about 40 and 120 minutes. The ootheca has an average of 13.4 (+/- 3.2 eggs. Incubation lasted for 34.1 days (+/- 1.97. Mean durations of total development from egg to adult was 156.2 days, being 34.1 in the egg stage, 53.4 in the ninphal stage, and 68.7 in the adult stage. Nymphs (N and adults (A accepted only six aquatic macrophytes out of 13 species offered during 30 days; survived more time in Pistia stratiotes (N = 43 %, 30 days and A = 1 %, 27 days and Pontederia sp. (N = 85 % and A = 40 % until 24 days than Limnobium sp. (N =13% and A =10 % until 12 dias, Ludwigia sp. (N =3 %, 24 days and A =15 %, 9 days Azolla sp. (N =35 %, 9 days and A =15 %, 12 days and Paspalum repens (N =3 %, 21 days and A =5 %, 24 days.

  17. Vigilancia Epidemiológica de Rickettsia‚ Ehrlichia y Anaplasma en Fauna Silvestre ex situ e in situ de algunas Regiones de Colombia -resumen-

    Directory of Open Access Journals (Sweden)

    Santiago Monsalve-Buriticá

    2014-12-01

    Full Text Available Las enfermedades causadas por bacterias del orden de los Rickettsiales son trasmitidas por ectoparásitos originados en vida silvestre‚ sin embargo‚ en Colombia no existe una línea base del conocimiento en este sentido. Estos microorganismos intracelulares son causantes de enfermedades transmisibles en pacientes humanos; convirtiéndolas en zoonosis de tipo emergente. Este estudio ha permitido determinar la presencia de microorganismos del orden de los Rickettsiales (Ehrlichia spp.‚ Anaplasma spp.‚ y Rickettsia spp. en ejemplares silvestres de centros de conservación ex situ (Zoológicos‚ CAV y CAVR y en algunas regiones en condiciones in situ del país. De igual manera se identifica la infección de estos microorganismos en ectoparásitos vectores relacionados. Por medio de la técnica PCRrt‚ se detectó la presencia de Rickettsia spp.‚ Anaplasma spp.‚ y Ehrlichia spp.‚ en suero obtenido de mamíferos‚ y garrapatas colectadas de reptiles y mamíferos del Centro de Atención y Valoración de Fauna silvestre de la Corporación Autónoma Regional de los Valles del Sinú y San Jorge CAV-CVS en Montería (Córdoba‚ en osos hormigueros (Myrmecophaga tridactyla en Pore (Casanare‚ y en garrapatas obtenidas en ejemplares de titi gris (Saguinus leucopus y su hábitat en la vereda La Parroquia en Mariquita (Tolima. En Montería‚ los resultados obtenidos en mamíferos demostraron la ausencia de Rickettsia spp. en 72 muestras de suero‚ sin embargo se detectó un pool de garrapatas de la especie Amblyomma ovale positivo a este microorganismo‚ siendo el primer reporte en Colombia de ectoparásitos de Puma concolor positivos a Rickettsia spp.‚ mientras que en reptiles se detectaron 18 pools de garrapatas positivos a Rickettsia spp (90 %. En las garrapatas colectadas de nueve osos palmeros (Myrmecophaga tridactyla de vida libre se detectaron 12 pool de garrapatas positivos a este microorganismo (17‚6 %‚ y en ejemplares de

  18. Composición y distribución espacio-temporal de las comunidades de arañas (Arachnida: Araneae en el sistema de cultivo maíz-soya de la altillanura plana colombiana, municipio de Puerto López, Meta

    Directory of Open Access Journals (Sweden)

    Flórez Daza Eduardo

    2004-12-01

    Full Text Available Actualmente la región de los Llanos Orientales de Colombia, la cual pertenece al sistema de importancia mundial de Sabanas Tropicales, ha sido escenario de la expansión agrícola en las últimas décadas debido al desarrollo de plantas genéticamente modificadas tolerantes al aluminio y a la política del Ministerio de Agricultura de suplir las necesidades internas de maíz y soya con producción nacional. Con los cambios que se están generando en esta zona y por la evidencia que la araneofauna puede actuar como reguladora de las poblaciones de insectos plaga, se planteó el presente trabajo cuyo objetivo principal fue determinar la composición
    y dinámica espacio-temporal de la fauna de arañas en el sistema de cultivo maíz-soya en el municipio de Puerto López, departamento del Meta. Para lo anterior se seleccionó una finca con producción comercial maíz-soya donde se efectuaron seis muestreos en diferentes épocas: soya-desarrollo vegetativo, soya-precosecha, maíz-fructificación, maíz-precosecha y soya-fructificación, entre octubre de 2001 y diciembre de 2002. Para efectuar los muestreos se diseñó una cuadrícula con un total de 70 sitios de lectura, distanciados entre si 175 m; estos
    puntos fueron georreferenciados respecto a un origen arbitrario en los lotes. La cuadrícula se centró en los cultivos de interés (maíz-soya e incorporó lecturas en los siguientes ecosistemas aledaños: bosque de sabana, morichal, sabana nativa y sabana introducida. Los métodos
    de captura empleados fueron trampas de caída (Pitfall y aspirador entomológico (Dvac. Se estimó la abundancia, diversidad (Shannon-Weaver y Simpson y riqueza en cada una de las etapas del cultivo y en cada uno de los ecosistemas. Además, esta información fue analizada
    por medio de técnicas geoestadísticas, con las que se evaluó la dinámica espacio-temporal de los gremios de la araneofauna colectada. En total se colectaron 1.653 ara

  19. Contaminantes orgánicos persistentes (COPs en leche materna de centros urbanos de la provincia de Buenos Aires

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    Lara Sofia Della Ceca

    2013-01-01

    Full Text Available Los contaminantes orgánicos persistentes (COPs se acumulan en matrices ricas en materia grasa como la leche materna, que es un buen indicador de sus niveles en poblaciones humanas debido a su fácil y no invasiva extracción. Con el objeto de evaluar la exposición a COPs en la provincia de Buenos Aires y compararla con otras  áreas, se analizaron bifenilos policlorados (PCBs, diclorodifeniltricloroetano (DDT y sus metabolitos (DDT, TDE, hexaclorociclohexanos (?, ? y ?-HCH y clordanos (CHLDs: heptaclor y su epóxido, trans y cis clordanos y nonaclors en muestras de leche materna colectadas durante 2010 y 2011 en Punta Lara, Ensenada, Florencio Varela y Capital Federal.Las  muestras fueron colectadas con sacaleches manuales, centrifugadas para separar la crema que fue liofilizada y extraída con éter de petróleo y ultrasonido. Los extractos previamente tratados con ácido para eliminación parcial de lípidos, fueron purificados por cromatografía en gel de sílice y analizados por cromatografía gaseosa.Las concentraciones de COPs en ng.g-1 lípido decrecieron en el orden DDTs (76±91 ? PCBs (67±64 > HCHs (33±36 ³ CHLDs (22±24.  Los DDTs oscilaron entre 7.7-510 ng.g-1 lípido y  los PCBs entre 5-247 ng.g-1 lípido, estos valores son comparables al rango más bajo reportado en la literatura (DDTs Noruega: 39-292 ng.g-1 lípido; PCBs Vietnam: 26-210 ng.g-1, Japón: 23-370 ng.g-1. En cambio, las concentraciones de HCHs y CHLDs, que oscilaron entre 5.8-197 ng.g-1 lípido y  1.3-124 ng.g-1 lípido respectivamente, corresponden al rango medio reportado para otras áreas (HCHs Indonesia 1.6-120 ng.g-1; CHLDs 3.4-92 ng.g-1. La composición de residuos en la leche materna es relativamente conservativa para cada clase de COPs, así se observa uniforme predominancia de productos de degradación (p.ej. DDE: 90±17% de SDDTs; epóxido de heptaclor: 50±18% y t-nonaclordano: 34±13% de SCHLDs y los isómeros y congéneres más persistentes (?-HCH

  20. Metal pollution (Pb, Zn, Ni and Cr) in air, road and soil sediment in a high traffic area; Contaminacion por metales (Pb, Zn, Ni y Cr) en aire, sedimentos viales y suelo en una zona de alto trafico vehicular

    Energy Technology Data Exchange (ETDEWEB)

    Manchado, Anali; Garcia, Neyma; Garcia, Cezar; Acosta, Lorena; Cordova, Alberto; Linares, Maria; Giraldoth, Debora [Centro de Investigaciones CEDEGAS, Facultad de Ingenieria, Universidad del Zulia, Maracaibo (Venezuela)]. E-mail: analimachado@yahoo.com; amachadop@luz.edu.ve; Velasquez, Harvi [Facultad Experimental de Ciencias, Universidad del Zulia, Maracaibo (Venezuela)

    2008-11-15

    Due to the important health effects of inhalable particles (PM10) and its associated metals, levels of four trace metals (Pb, Ni, Zn, Cr) generated by mobile sources with variable toxicity incorporated to PM10 atmospheric samples in a high density traffic area were evaluated. Samples were collected at two different sites (being considered as emission and dispersion areas) during a dry-wet season period, using a low volume air sampler equipped with quartz fiber filters; after an acid extraction, samples were analyzed by atomic absorption spectrometry. PM10 concentrations were two times higher in the emission area than in the dispersion area for both climatic regimens, but exhibited a better correlation between sites during the dry season. All the elements showed statistically significant differences between monitoring sites. On the other hand, when the total sampling period for PM10 is compared for all the tested metals, significant differences between sites and between meteorological seasons can be appreciated. The Venezuelan air quality standard for Pb was not exceeded, but a mean concentration of 1.13 {mu}g/m{sup 3} -very close to Venezuelan and USEPA standard- was obtained at the emission site. Nevertheless, Pb and Ni reported levels for both locations were above the Organizacion Mundial de la Salud (OMS) allowed limit during the whole sampling period. Further, the statistical analysis demonstrated the selection of the monitoring areas was adequate, confirming that motor vehicles are the main emission source of these atmospheric pollutants. [Spanish] Debido a la importancia del efecto sobre la salud de las particulas inhalables PM10 y los metales asociados a ellas, se evaluaron los niveles de cuatro metales traza (Pb, Ni, Zn y Cr) generados por fuentes moviles de dichas particulas, en sedimentos viales y suelo en una zona de alta densidad vehicular. Las muestras fueron colectadas en los sitios considerados como de emision y dispersion durante las epocas de

  1. Late cretaceous aquatic plant world in Patagonia, Argentina.

    Directory of Open Access Journals (Sweden)

    N Rubén Cúneo

    Full Text Available In this contribution, we describe latest Cretaceous aquatic plant communities from the La Colonia Formation, Patagonia, Argentina, based on their taxonomic components and paleoecological attributes. The La Colonia Formation is a geological unit deposited during a Maastrichtian-Danian transgressive episode of the South Atlantic Ocean. This event resulted in the deposition of a series of fine-grained sediments associated with lagoon systems occurring along irregular coastal plains in northern Patagonia. These deposits preserved a diverse biota, including aquatic and terrestrial plants and animals. The aquatic macrophytes can be broadly divided into two groups: free-floating and rooted, the latter with emergent or floating leaves. Free-floating macrophytes include ferns in Salviniaceae (Azolla and Paleoazolla and a monocot (Araceae. Floating microphytes include green algae (Botryoccocus, Pediastrum and Zygnemataceae. Among the rooted components, marsileaceous water ferns (including Regnellidium and an extinct form and the eudicot angiosperm Nelumbo (Nelumbonaceae are the dominant groups. Terrestrial plants occurring in the vegetation surrounding the lagoons include monocots (palms and Typhaceae, ferns with affinities to Dicksoniaceae, conifers, and dicots. A reconstruction of the aquatic plant paleocommuniy is provided based on the distribution of the fossils along a freshwater horizon within the La Colonia Formation. This contribution constitutes the first reconstruction of a Cretaceous aquatic habitat for southern South America.

  2. Heavy metals in water, sediments and wetland plants in an aquatic ecosystem of tropical industrial region, India.

    Science.gov (United States)

    Rai, Prabhat Kumar

    2009-11-01

    Concentrations of heavy metals (Cu, Cr, Fe, Pb, Zn, Hg, Ni, and Cd) and macronutrients (Mn) were measured in industrial effluents, water, bottom sediments, and wetland plants from a reservoir, Govind Ballabh (G.B.) Pant Sagar, in Singrauli Industrial region, India. The discharge point of a thermal power plant, a coal mine, and chlor-alkali effluent into the G.B. Pant Sagar were selected as sampling sites with one reference site in order to compare the findings. The concentrations of heavy metals in filtered water, sieved sediment samples (0.4-63 microm), and wetland plants were determined with particle-induced X-ray emission. The collected plants were Aponogeton natans, L. Engl. & Krause, Cyperus rotundus, L., Hydrilla verticillata, (L.f.) Royle, Ipomoea aquatica, Forssk., Marsilea quadrifolia, L., Potamogeton pectinatus, L., Eichhornia crassipes, (Mart.) Solms Monogr., Lemna minor, L., Spirodela polyrhiza (L.) Schleid. Linnaea, Azolla pinnata, R.Br., Vallisneria spiralis, L., and Polygonum amphibium, L. In general, metal concentration showed a significant positive correlation between industrial effluent, lake water, and lake sediment (p < 0.01). Likewise, significant positive correlation was recorded with metals concentration in plants and lake ambient, which further indicated the potential of aforesaid set of wetland macrophytes for pollution monitoring. PMID:18998227

  3. Arsenic uptake and speciation in the rootless duckweed Wolffia globosa.

    Science.gov (United States)

    Zhang, Xin; Zhao, Fang-Jie; Huang, Qing; Williams, Paul N; Sun, Guo-Xin; Zhu, Yong-Guan

    2009-01-01

    Duckweeds are a common macrophyte in paddy and aquatic environments. Here, we investigated arsenic (As) accumulation, speciation and tolerance of the rootless duckweed Wolffia globosa and its potential for As phytofiltration. When grown with 1 microm arsenate, W. globosa accumulated two to 10 times more As than four other duckweed or Azolla species tested. W. globosa was able to accumulate > 1000 mg As kg(-1) in frond dry weight (DW), and tolerate up to 400 mg As kg(-1) DW. At the low concentration range, uptake rate was similar for arsenate and arsenite, but at the high concentration range, arsenite was taken up at a faster rate. Arsenite was the predominant As species (c. 90% of the total extractable As) in both arsenate- and arsenite-exposed duckweed. W. globosa was more resistant to external arsenate than arsenite, but showed a similar degree of tolerance internally. W. globosa decreased arsenate in solution rapidly, but also effluxed arsenite. Wolffia globosa is a strong As accumulator and an interesting model plant to study As uptake and metabolism because of the lack of a root-to-frond translocation barrier. PMID:19210724

  4. Occurrence of arsenic species in algae and freshwater plants of an extreme arid region in northern Chile, the Loa River Basin.

    Science.gov (United States)

    Pell, Albert; Márquez, Anna; López-Sánchez, José Fermín; Rubio, Roser; Barbero, Mercedes; Stegen, Susana; Queirolo, Fabrizio; Díaz-Palma, Paula

    2013-01-01

    This study reports data on arsenic speciation in two green algae species (Cladophora sp. and Chara sp.) and in five aquatic plants (Azolla sp., Myriophyllum aquaticum, Phylloscirpus cf. desserticola, Potamogeton pectinatus, Ruppia filifolia and Zannichellia palustris) from the Loa River Basin in the Atacama Desert (northern Chile). Arsenic content was measured by Mass spectrometry coupled with Inductively Coupled Plasma (ICP-MS), after acidic digestion. Liquid chromatography coupled to ICP-MS was used for arsenic speciation, using both anionic and cationic chromatographic exchange systems. Inorganic arsenic compounds were the main arsenic species measured in all samples. The main arsenic species in the extracts of freshwater algae and plants were arsenite and arsenate, whereas glycerol-arsenosugar (gly-sug), dimethylarsinic acid (DMA) and methylarsonic acid (MA) were present only as minor constituents. Of the samples studied, algae species accumulated more arsenic than aquatic plants. Total arsenic content ranged from 182 to 11100 and from 20 to 248 mg As kg(-1) (d.w.) in algae and freshwater plants, respectively. In comparison with As concentration in water samples, there was hyper-accumulation (>0.1% d.w.) in Cladophora sp. PMID:22981629

  5. Heavy metal pollution in aquatic ecosystems and its phytoremediation using wetland plants: An ecosustainable approach

    Energy Technology Data Exchange (ETDEWEB)

    Rai, P.K. [Mizoram Central University, Tanhril (India). School for Earth Science & Natural Resource Management

    2008-07-01

    This review addresses the global problem of heavy metal pollution originating from increased industrialization and urbanization and its amelioration by using wetland plants both in a microcosm as well as natural/field condition. This review mentions salient features of wetland ecosystems, their vegetation component, and the pros and cons involved in heavy metal removal. Wetland plants are preferred over other bio-agents due to their low cost, frequent abundance in aquatic ecosystems, and easy handling. Constructed wetlands proved to be effective for the abatement of heavy metal pollution from acid mine drainage; landfill leachate; thermal power; and municipal, agricultural, refinery, and chlor-alkali effluent. the physicochemical properties of wetlands provide many positive attributes for remediating heavy metals. Typha, Phragmites, Eichhornia, Azolla, Lemna, and other aquatic macrophytes are some of the potent wetland plants for heavy metal removal. Biomass disposal problem and seasonal growth of aquatic macrophytes are some limitations in the transfer of phytoremediation technology from the laboratory to the field. However, the disposed biomass of macrophytes may be used for various fruitful applications. An ecosustainable model has been developed through the author's various works, which may ameliorate some of the limitations. The creation of more areas for phytoremediation may also aid in wetlands conservation. Genetic engineering and biodiversity prospecting of endangered wetland plants are important future prospects in this regard.

  6. 满江红多胺氧化酶的抽提与初步纯化

    Institute of Scientific and Technical Information of China (English)

    何生根; 欧利叶; 等

    1998-01-01

    报告了满江红(Azolla imbricata)多胺氧化酶提取条件初步纯化的研究结果,以0.1mol/L磷酸缓冲液为介质抽提该酶时的最适PH为6.5,添加0.10-0.15mol/L NaCl和2%-4%(质量体积比)PVP可改善抽提效果,用硫酸铵沉淀法、PEG-6000沉淀法和丙酮沉淀法分别对酶抽提液进行初步纯化,发现丙酮沉淀法化效果最优,用1.2倍(体积比)冷丙酮(-15℃)沉淀时可提纯该酶8.92倍,产率达74.6%。

  7. An Innovative Device to Convert Olive Mill Wastewater into a Suitable Effluent for Feeding Purple Non-Sulfur Photosynthetic Bacteria

    Directory of Open Access Journals (Sweden)

    Pietro Carlozzi

    2015-08-01

    Full Text Available A device (prototype with a working volume of 200 L was used to deplete olive mill wastewater (OMW of polyphenols. The OMW transformed into feedstock by means of the device was then used for feeding a lab-scale photobioreactor, just for testing the production of bioH2. The main novelty of this prototype consists in the combination of several adsorbent matrices and the exploitation of their synergic action. In this investigation, three matrices have been used: active carbon, Azolla and zeolite. The device was operated at an olive oil company located in the heart of the Chianti zone (Province of Florence, Italy. The efficiency of polyphenol removal obtained using the device was ≥96%. The multi-matrix effluent (MMeff generated was then used to obtain three different culture broths containing 25%, 50% and 100% of MMeff, respectively. The diluted (with water culture broths were suitable for hydrogen generation, with the highest hydrogen production rate (12.7 mL H2/Lculture/h being obtained using 50% MMeff. The hydrogen yields were: 334 mL H2/L of MMeff, when feeding the photofermenter with pure effluent (100%; 1308 mL H2/L of MMeff, with the half-diluted effluent (50%, v/v; and 432 mL H2/L of MMeff, with the highest-diluted effluent (25%, v/v.

  8. Dephenolization of stored olive-mill wastewater, using four different adsorbing matrices to attain a low-cost feedstock for hydrogen photo-production.

    Science.gov (United States)

    Padovani, Giulia; Pintucci, Cristina; Carlozzi, Pietro

    2013-06-01

    This investigation deals with the conversion of olive-mill wastewater (OMW) into several feedstocks suitable for hydrogen photo-production. The goal was reached by means of two sequential steps: (i) a pre-treatment process of stored-OMW for the removal of polyphenols, which made it possible to obtain several effluents, and (ii) a photo-fermentative process for hydrogen production by means of Rhodopseudomonas palustris sp. Four different adsorbent matrices (Azolla, granular active carbon, resin, and zeolite) were used to dephenolize stored-OMW. The four liquid fractions attained by using the above process created the same number of effluents, and these were diluted with water and then used for hydrogen photo-production. The maximum hydrogen production rate (14.31 mL/L/h) was attained with the photo-fermenter containing 25% of the effluent, which came from the pre-treatment of stored-OMW using granular active carbon. Using the carbon effluent as feedstock, the greatest light conversion efficiency of 2.29% was achieved. PMID:23612177

  9. 满江红多胺氧化酶部分特性研究

    Institute of Scientific and Technical Information of China (English)

    何生根; 黄学林; 等

    1996-01-01

    本文报告了满江红(Azolla imbricata)多胺氧化酶特性的一些研究结果,该酶的最适底物为spd(亚精胺),其次为spm(精胺)(酶活性约为以spd为底物时的405),而对put(腐胺)和cad(尸胺)两种二胺几乎不氧化;在50-70℃或PH4-10的范围内均有活性,以spd和spm作底物时的最适PH最分别为6.5和5.5;该酶经10mmol/L的EDTA处理后酶活性仍能完全保持,说明其催化功能的实现可能无需金属离子的参与。另外,金属离子Ca2+,Mg2+对该酶活性无明显影响,但10mol/L的CuSO4可抑制约20%的活性。

  10. Removal and accumulation of mercury by aquatic macrophytes from an open cast coal mine effluent.

    Science.gov (United States)

    Mishra, Virendra Kumar; Tripathi, B D; Kim, Ki-Hyun

    2009-12-30

    In this study, the mercury (Hg) removal capacities of two aquatic macrophytes, Pistia stratiotes and Azolla pinnata, were investigated against the coal mining effluent. These plants reduced mercury from the effluent via rhizofiltration and subsequent accumulation in plant. The removal rate of P. stratiotes and A. pinnata was 80% and 68%, respectively, after 21 days of exposure to the effluent containing 10 microg L(-1) of Hg. As mercury from the effluent was accumulated in the root and shoot tissues of both aquatic macrophytes, they were proven to be a root accumulator with a translocation factor of less than one during the entire study. The decreasing Hg content in effluent (from 10 to 2.0 microg L(-1)) was reflected by its accumulation in roots (0.57+/-0.02 mg g(-1) in P. stratiotes) and leaves of the experimental plants (0.42+/-0.01 mg g(-1), P. stratiotes). As a result, Hg concentrations in the coal mining effluent were tightly associated with those observed from macrophytes. Considering the high removal efficiencies of Hg by these aquatic macrophytes, these plants can be recommended for the actual treatment of Hg-containing waste waters. PMID:19665290

  11. Current approaches of the management of mercury poisoning: need of the hour.

    Science.gov (United States)

    Rafati-Rahimzadeh, Mehrdad; Rafati-Rahimzadeh, Mehravar; Kazemi, Sohrab; Moghadamnia, Ali Akbar

    2014-01-01

    Mercury poisoning cases have been reported in many parts of the world, resulting in many deaths every year. Mercury compounds are classified in different chemical types such as elemental, inorganic and organic forms. Long term exposure to mercury compounds from different sources e.g. water, food, soil and air lead to toxic effects on cardiovascular, pulmonary, urinary, gastrointestinal, neurological systems and skin. Mercury level can be measured in plasma, urine, feces and hair samples. Urinary concentration is a good indicator of poisoning of elemental and inorganic mercury, but organic mercury (e.g. methyl mercury) can be detected easily in feces. Gold nanoparticles (AuNPs) are a rapid, cheap and sensitive method for detection of thymine bound mercuric ions. Silver nanoparticles are used as a sensitive detector of low concentration Hg2+ ions in homogeneous aqueous solutions. Besides supportive therapy, British anti lewisite, dimercaprol (BAL), 2,3-dimercaptosuccinic acid (DMSA. succimer) and dimercaptopropanesulfoxid acid (DMPS) are currently used as chelating agents in mercury poisoning. Natural biologic scavengers such as algae, azolla and other aquatic plants possess the ability to uptake mercury traces from the environment. PMID:24888360

  12. Depositional environments of the Hart coal zone (Paleocene), Willow Bunch Coalfield, southern Saskatchewan, Canada from petrographic, palynological, paleobotanical, mineral and trace element studies

    Energy Technology Data Exchange (ETDEWEB)

    Potter, J.; Beaton, A.P.; McDougall, W.J.; Nambudiri, E.M.V.; Vigrass, L.W. (University of Regina, SK (Canada). Energy Research Unit)

    1991-12-01

    Coal petrology, palynology, paleobotany and mineralogy of the Hart coal indicate deposition under wet, warm-temperate to subtropical climatic conditions in low-lying backswamps with fluvial channels and locally ponded areas. The coal is dominated by mixed xylitic/attrital lithotypes and by huminite macerals with secondary inertinite macerals and minor liptinite macerals. Good correlation exists between lithotypes and maceral composition. Local and vertical variations in proportions of huminites and inertinites reflect frequent fluctuations in water levels, periodic flooding, desiccation and burning of the peat. Swamps were dominated by {ital Glyptostrobus-Taxodium} forest with {ital Betula-Myrica-Alnus} communities and, locally {ital Laevigatosporites}, which are the dominant contributors to the xylite-rich lithotypes. Attrital lithotypes with abundant {ital Pandanus}, {ital Typha} and {ital Azolla} are consistent with wetter areas of a fluvial environment, including ponds and channels. Trace elements Cr, Cu, Mo, Ni, Si, Ti, U, Se, V, W, K and Th, typically associated with syngenetic minerals kaolinite, calcite and quartz, may have a volcanic source. High concentrations of Na, Ba and Ca found in organic complexes are of secondary origin and probably originate in deep source brines rather than marine surface waters. 55 refs., 11 figs., 5 tabs.

  13. The Middle and Upper Eocene sections of the Omsk trough, West Siberian Platform: Palynological, stratigraphic, hydrologic, and climatic aspects

    Science.gov (United States)

    Zaporozhets, N. I.; Akhmetiev, M. A.

    2013-01-01

    The thorough analysis and correlation of Middle-Upper Eocene sections in the Omsk trough (southern West Siberian Platform) recovered by Borehole 9 in its axial part near the Chistoozernoe Settlement (Novosibirsk region) and Borehole 8 on the southern limb near the Russkaya Polyana Settlement (southern Omsk region) revealed hiatuses at the base and top of the Russkaya Polyana Beds, a lithostratigraphic unit defined in the Lyulinvor Formation based on its substantially fine-grained composition and poor siliceous microplankton fossil remains. The overlying Tavda Formation (Middle-Upper Eocene) is traditionally accepted to consist of two subformations. The last formation was deposited in the West Siberian inner sea isolated from the Arctic basin. Particular attention is paid to eustatic sea level fluctuation especially during the period marked by accumulation of Azolla Beds under considerable desalination of surface waters in the basin. The curve of variations in the open sea factor based on the quantitative ratio between organic-walled phytoplankton fossils and higher plant palynomorphs is correlated with the modified version of the wellknown Vail curve. It is established that the West Siberian sea level experienced a brief rise in the terminal late Eocene prior to its complete desiccation at the Eocene-Oligocene transition because of global regression in response to glaciation in Antarctica.

  14. Impact of Formulated Protein Diets on Growth of the Indian Major Carp, Labeo rohita (Hamilton

    Directory of Open Access Journals (Sweden)

    S Manivannan

    2012-01-01

    Full Text Available Fresh water aquaculture in India is mainly carp-based and accounts for a considerable proportion of total aquaculture production. Feeding constitutes a major factor in fish culture since the fish obtain the maximum nutritional requirement through the food they consume. Protein forms one of the main components of fish feed and hence formulation of feeds that contain high amount of protein using cost-effective natural ingredients is very essential to achieve efficient production from fish culture. Labeo rohita is an extensively studied fish and contributes to a considerable proportion of fish production in India. In the present investigation, four different kinds of fish feed (using groundnut cake, azolla, rice bran and tapioca powder were formulated with varying protein concentrations (30%, 35%, 40% and 45% and given to L. rohita to assess the impact of the protein diet on its growth and bioenergetic parameters. The fish were fed with the formulated feed at a rate of 4% of their body weight for a period of 60 days. It was observed that the fish fed with formulated feed containing 40% protein content showed better growth results and improvement in bioenergetic parameters, compared to the other three feeds.

  15. Late cretaceous aquatic plant world in Patagonia, Argentina.

    Science.gov (United States)

    Cúneo, N Rubén; Gandolfo, María A; Zamaloa, María C; Hermsen, Elizabeth

    2014-01-01

    In this contribution, we describe latest Cretaceous aquatic plant communities from the La Colonia Formation, Patagonia, Argentina, based on their taxonomic components and paleoecological attributes. The La Colonia Formation is a geological unit deposited during a Maastrichtian-Danian transgressive episode of the South Atlantic Ocean. This event resulted in the deposition of a series of fine-grained sediments associated with lagoon systems occurring along irregular coastal plains in northern Patagonia. These deposits preserved a diverse biota, including aquatic and terrestrial plants and animals. The aquatic macrophytes can be broadly divided into two groups: free-floating and rooted, the latter with emergent or floating leaves. Free-floating macrophytes include ferns in Salviniaceae (Azolla and Paleoazolla) and a monocot (Araceae). Floating microphytes include green algae (Botryoccocus, Pediastrum and Zygnemataceae). Among the rooted components, marsileaceous water ferns (including Regnellidium and an extinct form) and the eudicot angiosperm Nelumbo (Nelumbonaceae) are the dominant groups. Terrestrial plants occurring in the vegetation surrounding the lagoons include monocots (palms and Typhaceae), ferns with affinities to Dicksoniaceae, conifers, and dicots. A reconstruction of the aquatic plant paleocommuniy is provided based on the distribution of the fossils along a freshwater horizon within the La Colonia Formation. This contribution constitutes the first reconstruction of a Cretaceous aquatic habitat for southern South America. PMID:25148081

  16. Fluoride remediation using floating macrophytes

    Directory of Open Access Journals (Sweden)

    Naba Kumar Mondal

    2014-04-01

    Full Text Available Six aquatic macrophytes, such as Pistia stratiotes, Ceratophyllum demersum, Nymphoides indica, Lemna major, Azolla pinnata,and Eichhornia crassipes were considered for remove fluoride from aqueous solution. Five different concentrations (10, 30, 50, and 100 ppm of fluoride solution were taken in 1 L plastic container. Fixed weight (20 g of macrophytes along with 500 ml fluoride solution was taken in each plastic container for 72 hours observation. Results demonstrated all the macrophytes show highest fluoride removal during 24 h to 48 h, but after 72 h their efficiency reduced drastically. The species N. indica showed better removal efficiency than other experimental macrophytes. In general, pigment measurement data indicated higher concentration at 72 h. However, Pistia sp. showed higher concentration of pigmentation at intermediate time interval (48 h. Higher level of dry weight to fresh weight ratio was recorded for L. major and A. pinnata at all concentrations, excepting at 10 ppm. In addition, all macrophytes showed lower RGR at higher concentration. Isotherm study indicated that macrophyte C. demersum is a good fitted with Freundlich and Langmuir isotherm whereas L. major with Langmuir isotherm during 24 hours.

  17. Phytoremediation of the toxic effluent generated during recovery of precious metals from polymetallic sea nodules.

    Science.gov (United States)

    Vaseem, Huma; Banerjee, T K

    2012-01-01

    Recovery of metals from the polymetallic sea nodules at the pilot plant at National Metallurgical Laboratory, Jamshedpur, India has generated a highly toxic effluent. This effluent contains several metals like Mn, Cu, Zn, Fe, Pb, Cr, and Cd that pollute the neighboring water bodies when discharged. Hence detoxification of this effluent was practiced using two plants: Lemna minor and Azolla pinnata for 7 days. During investigation A. pinnata removed 96% of Mn, 97% of Cu, 98% of Zn, 70% of Fe, 96% of Pb, 93% of Cr, 78% of Cd, and was comparatively more effective than L. minor which removed 94% of Mn, 86% of Cu, 62% of Zn, 74% of Fe, 84% of Pb, 63% of Cr, 78% of Cd. During the 7 days of experiment chlorophyll content decreased by 51% and 59% in A. pinnata and L. minor respectively. Based on our findings we can suggest that these two plants have wide range of metal retention potentialities hence can be of routine use for purification of toxic effluents. PMID:22567724

  18. Water isotopes and the Eocene. A tectonic sensitivity study

    Science.gov (United States)

    Legrande, A. N.; Roberts, C. D.; Tripati, A.; Schmidt, G. A.

    2009-04-01

    The early Eocene (54 Million years ago) is one of the warmest periods in the last 65 Million years. Its climate is postulated to have been the result of enhanced greenhouse gas concentration, with CO2 roughly 4 times pre-industrial and methane 7 times pre-industrial concentrations. One interesting feature of this period to emerge recently is the intermittent presence of fossilized Azolla, a type of freshwater fern, in the Arctic Ocean. Synchronous (within dating error) with this appearance were major changes in the restriction of the Arctic Ocean and the other global oceans. We investigate this time period using the Goddard Institute for Space Studies ModelE-R, a fully coupled atmosphere-ocean general circulation model that incorporates water isotopes throughout the hydrologic cycle, making it an ideal model to test hypotheses of past climate change and to compare to paleoclimate proxy data. We assess the impact of tectonic variability by using minimal and maximal levels of restriction for the Arctic Ocean seaways. We find that the modulation of connectivity of these basins dramatically alters global salinity distribution, leading to large changes in ocean circulation. Greater restriction of the Arctic Basin is associated with fresh and relatively warmer conditions. The same mechanisms responsible for this redistribution of salt also change the global distribution of water isotopes, and can alias (water isotope) proxy climate signals of warmth.

  19. Abiotic and biotic factors that influence the bioavailability of gold nanoparticles to aquatic macrophytes.

    Science.gov (United States)

    Glenn, J Brad; Klaine, Stephen J

    2013-09-17

    This research identified and characterized factors that influenced nanomaterial bioavailability to three aquatic plants: Azolla caroliniana Willd, Egeria densa Planch., and Myriophyllum simulans Orch. Plants were exposed to 4-, 18-, and 30-nm gold nanoparticles. Uptake was influenced by nanoparticle size, the presence of roots on the plant, and dissolved organic carbon in the media. Statistical analysis of the data also revealed that particle uptake was influenced by a 4-way (plant species, plant roots, particle size, and dissolved organic carbon) interaction suggesting nanoparticle bioavailability was a complex result of multiple parameters. Size and species dependent absorption was observed that was dependent on the presence of roots and nanoparticle size. The presence of dissolved organic carbon was found to associate with 4- and 18-nm gold nanoparticles in suspension and form a nanoparticle/organic matter complex that resulted in (1) minimized particle aggregation and (2) a decrease of nanoparticle absorption by the aquatic plants. The same effect was not observed with the 30-nm nanoparticle treatment. These results indicate that multiple factors, both biotic and abiotic, must be taken into account when predicting bioavailability of nanomaterials to aquatic plants. PMID:23947987

  20. Assessment of potential indigenous plant species for the phytoremediation of arsenic-contaminated areas of Bangladesh.

    Science.gov (United States)

    Mahmud, Rezwanul; Inoue, Naoto; Kasajima, Shin-Ya; Shaheen, Riffat

    2008-01-01

    Soil and water contaminated with arsenic (As) pose a major environmental and human health problem in Bangladesh. Phytoremediation, a plant-based technology, may provide an economically viable solution for remediating the As-polluted sites. The use of indigenous plants with a high tolerance and accumulation capacity for As may be a very convenient approach for phytoremediation. To assess the potential of native plant species for phytoremediation, plant and soil samples were collected from four As-contaminated (groundwater) districts in Bangladesh. The main criteria used for selecting plants for phytoremediation were high bioconcentration factors (BCFs) and translocation factors (TFs) of As. From the results of a screening of 49 plant species belonging to 29 families, only one species of fern (Dryopteris filix-mas), three herbs (Blumea lacera, Mikania cordata, and Ageratum conyzoides), and two shrubs (Clerodendrum trichotomum and Ricinus communis) were found to be suitable for phytoremediation. Arsenic bioconcentration and translocation factors > 1 suggest that these plants are As-tolerant accumulators with potential use in phytoextraction. Three floating plants (Eichhornia crassipes, Spirodela polyrhiza, and Azolla pinnata) and a common wetland weed (Monochoria vaginalis) also showed high BCF and TF values; therefore, these plants may be promising candidates for cleaningup As-contaminated surface water and wetland areas. The BCF of Oryza sativa, obtained from As-contaminated districts was > 1, which highlights possible food-chain transfer issues for As-contaminated areas in Bangladesh. PMID:18709925

  1. Between two fern genomes.

    Science.gov (United States)

    Sessa, Emily B; Banks, Jo Ann; Barker, Michael S; Der, Joshua P; Duffy, Aaron M; Graham, Sean W; Hasebe, Mitsuyasu; Langdale, Jane; Li, Fay-Wei; Marchant, D Blaine; Pryer, Kathleen M; Rothfels, Carl J; Roux, Stanley J; Salmi, Mari L; Sigel, Erin M; Soltis, Douglas E; Soltis, Pamela S; Stevenson, Dennis W; Wolf, Paul G

    2014-01-01

    Ferns are the only major lineage of vascular plants not represented by a sequenced nuclear genome. This lack of genome sequence information significantly impedes our ability to understand and reconstruct genome evolution not only in ferns, but across all land plants. Azolla and Ceratopteris are ideal and complementary candidates to be the first ferns to have their nuclear genomes sequenced. They differ dramatically in genome size, life history, and habit, and thus represent the immense diversity of extant ferns. Together, this pair of genomes will facilitate myriad large-scale comparative analyses across ferns and all land plants. Here we review the unique biological characteristics of ferns and describe a number of outstanding questions in plant biology that will benefit from the addition of ferns to the set of taxa with sequenced nuclear genomes. We explain why the fern clade is pivotal for understanding genome evolution across land plants, and we provide a rationale for how knowledge of fern genomes will enable progress in research beyond the ferns themselves. PMID:25324969

  2. Concentrations of heavy metals and aquatic macrophytes of Govind Ballabh Pant Sagar an anthropogenic lake affected by coal mining effluent.

    Science.gov (United States)

    Mishra, Virendra Kumar; Upadhyay, Alka Rani; Pandey, Sudhir Kumar; Tripathi, B D

    2008-06-01

    Five heavy metals Cu, Cd, Mn, Pb and Hg were found in high concentration from three sampling sites located in Asia's largest anthropogenic lake Govind Ballabh Pant GBP Sagar. Concentrations of these heavy metals were measured in Water, bottom sediment and in different parts of the aquatic macrophytes collected from the reservoir. Plants collected from the lake were Eichhornia crassipes, Azolla pinnata, Lemna minor, Spirodela polyrrhiza, Potamogeton pectinatus, Marsilea quadrifolia, Pistia stratiotes, Ipomea aquqtica, Potamogeton crispus, Hydrilla verticillata and Aponogeton natans. These plants have shown the high concentrations of Cu, Cd, Mn, Pb and Hg in their different parts due to bioaccumulation. In general plant roots exhibited higher concentrations of heavy metals than corresponding sediments. A comparison between different morphological tissues of the sampled plants revealed the metal concentration in following order roots > leaves. Analyses of bottom sediment indicated the higher concentrations of Cd, Mn, Cu and Pb. Strong positive correlations were obtained between the metals in water and in plants as well as between metal in sediment and in plants. Indicating the potential of these plants for pollution monitoring of these metals. PMID:17674134

  3. Assessment of native plant species for phytoremediation of heavy metals growing in the vicinity of NTPC sites, Kahalgaon, India.

    Science.gov (United States)

    Kumari, Alka; Lal, Brij; Rai, Upendra Nath

    2016-01-01

    The present investigation was carried out to screen native plants growing in fly ash (FA) contaminated areas near National Thermal Power Corporation (NTPC), Kahalgaon, Bihar, India with a view to using them for the eco-restoration of the area. A total number of 30 plant species (5 aquatic and 25 terrestrial including 6 ferns) were collected and their diversity status and dominance were also studied. After screening of dominant species at highly polluted site, 8 terrestrial and 5 aquatic plants were analyzed for heavy metals (Fe, Zn, Cu, Ni, Si, Al, Pb, Cr, and Cd). Differential accumulations of various heavy metals by different species of plants were observed. Typha latifolia was found to be most efficient metal accumulator of Fe (927), Cu (58), Zn (87), Ni (57), Al (67), Cd (95), and Pb (69), and Azolla pinnata as Cr (93) hyper-accumulator among aquatic species in µg g(-1). In terrestrial species the maximum levels of Fe (998), Zn (81), Ni (93), Al (121), and Si (156) were found in Croton bonplandium. However, there was high spatial variability in total metal accumulation in different species indicated by coefficient of variation (CV%). These results suggest that various aquatic, some dominant terrestrial plants including fern species may be used in a synergistic way to remediate and restore the FA contaminated wastelands. PMID:26442874

  4. Use of cyanobacterial diazotrophic technology in rice agriculture

    Energy Technology Data Exchange (ETDEWEB)

    Tiwari, D.N.; Kumar, A.; Mishra, A.K. [Banaras Hindu Univ., Varanasi (India)

    1991-12-31

    Diazotrophic cyanobacteria are photoautotrophic organisms that require sunlight as a sole energy source for the fixation of carbon and nitrogen. Therefore, they have great potential as biofertilizers, and their use will decrease fuel demand for fertilizer production. The agronomic potential of heterocystous cyanobacteria, either free-living or in symbiotic association with water fern Azolla, has long been recognized. This has led to the development of small scale biotechnology involving the use of paddy soils with appropriate cyanobacterial strains as biofertilizers in rice culture, as has been reported from China, Egypt, Philippines, and India. Besides increasing soil fertility and sustaining rice yield, these forms are also reported to benefit rice seedlings by producing growth-promoting substances, the nature of which is said to resemble gibberellins. Whereas the incorporation of nif genes into the rice plants by using tissue culture and modern genetic tools remain one of the ambitious research goals, the use of cyanobacterial diazotrophic technology in rice agriculture offers an immediate or even long-term alternative to synthetic nitrogen fertilizers, particularly in developing countries and the world as a whole. However, one of the weaknesses in this technology is the heavy application of several toxic agrochemicals, especially herbicides, which are reported in most cases as inhibitors of cyanobacterial diazotrophic growth, and in some cases as mutagenic. Naturally, a successful biotechnology requires the selection of suitable diazotrophic strains, as biofertilizers, that could tolerate the field-dose concentrations of herbicides and secrete ammonia.

  5. Accumulation of arsenic by aquatic plants in large-scale field conditions: opportunities for phytoremediation and bioindication.

    Science.gov (United States)

    Favas, Paulo J C; Pratas, João; Prasad, M N V

    2012-09-01

    This work focuses on the potential of aquatic plants for bioindication and/or phytofiltration of arsenic from contaminated water. More than 71 species of aquatic plants were collected at 200 sampling points in running waters. The results for the 18 most representative plant species are presented here. The species Ranunculus trichophyllus, Ranunculus peltatus subsp. saniculifolius, Lemna minor, Azolla caroliniana and the leaves of Juncus effusus showed a very highly significant (P<0.001) positive correlation with the presence of arsenic in the water. These species may serve as arsenic indicators. The highest concentration of arsenic was found in Callitriche lusitanica (2346 mg/kg DW), Callitriche brutia (523 mg/kg DW), L. minor (430 mg/kg DW), A. caroliniana (397 mg/kg DW), R. trichophyllus (354 mg/kg DW), Callitriche stagnalis (354 mg/kg DW) and Fontinalis antipyretica (346 mg/kg DW). These results indicate the potential application of these species for phytofiltration of arsenic through constructed treatment wetlands or introduction of these plant species into natural water bodies. PMID:22820614

  6. Phytoremediation of heavy metals in a tropical impoundment of industrial region.

    Science.gov (United States)

    Rai, Prabhat Kumar

    2010-06-01

    Aquatic pollution poses a serious challenge to the scientific community worldwide, since lakes or reservoirs find multifarious use and most often their water is used for drinking, bathing, irrigation, and aquaculture. Nine metals and several physicochemical parameters, from four sampling sites in a tropical lake receiving the discharges from a thermal power plant, a coal mine, and a chlor-alkali industry, were studied from 2004 to 2005. Pertaining to metal pollution, the site most polluted with heavy metals was Belwadah, i.e., waters and sediments had the highest concentration of all the metals examined. The reference site was characterized by the presence of low concentrations of metals in waters and sediments. Following the water quality monitoring, 2-month field phytoremediation experiments were conducted using large enclosures at the discharge point of different polluted sites of the lake. During field phytoremediation experiments using aquatic macrophytes, marked percentage reduction in metals concentrations were recorded. The percentage decrease for different metals was in the range of 25% to 67.90% at Belwadah (with Eichhornia crassipes and Lemna minor), 25% to 77.14% at Dongia nala (with E. crassipes, L. minor and Azolla pinnata), and 25% to 71.42% at Ash pond site of G.B. Pant Sagar (with L. minor and A. pinnata). Preliminary studies of polluted sites are useful for improved microcosm design and for the systematic extrapolation of information from experimental ecosystems to natural ecosystems. PMID:19430918

  7. Deciphering the factors associated with the colonization of rice plants by cyanobacteria.

    Science.gov (United States)

    Bidyarani, Ngangom; Prasanna, Radha; Chawla, Gautam; Babu, Santosh; Singh, Rajendra

    2015-04-01

    Cyanobacteria-rice plant interactions were analyzed using a hydroponics experiment. The activity of plant defense and pathogenesis-related enzymes, scanning electron microscopy, growth, nitrogen fixation (measured as ARA), and DNA fingerprinting assays proved useful in illustrating the nature of associations of cyanobacteria with rice plants. Microscopic analyses revealed the presence of short filaments and coiled masses of filaments of cyanobacteria near the epidermis and cortex of roots and shoot tissues. Among the six cyanobacterial strains employed, Calothrix sp. (RPC1), Anabaena laxa (RPAN8), and Anabaena azollae (C16) were the best performing strains, in terms of colonization in roots and stem. These strains also enhanced nitrogen fixation and stimulated the activity of plant defense/cell wall-degrading enzymes. A significantly high correlation was also recorded between the elicited plant enzymes, growth, and ARA. DNA fingerprinting using highly iterated palindromic sequences (HIP-TG) further helped in proving the establishment of inoculated organisms in the roots/shoots of rice plants. This study illustrated that the colonization of cyanobacteria in the plant tissues is facilitated by increased elicitation of plant enzymes, leading to improved plant growth, nutrient mobilization, and enhanced plant fitness. Such strains can be promising candidates for developing "cyanobacteria colonized-nitrogen-fixing rice plants" in the future.

  8. Management of fresh water weeds (macrophytes) by vermicomposting using Eisenia fetida.

    Science.gov (United States)

    Najar, Ishtiyaq Ahmed; Khan, Anisa B

    2013-09-01

    In the present study, potential of Eisenia fetida to recycle the different types of fresh water weeds (macrophytes) used as substrate in different reactors (Azolla pinnata reactor, Trapa natans reactor, Ceratophyllum demersum reactor, free-floating macrophytes mixture reactor, and submerged macrophytes mixture reactor) during 2 months experiment is investigated. E. fetida showed significant variation in number and weight among the reactors and during the different fortnights (P weeds) into three clusters-poor vermicompost substrates, moderate vermicompost substrate, and excellent vermicompost substrate. Two principal components (PCs) have been identified by factor analysis with a cumulative variance of 90.43 %. PC1 accounts for 47.17 % of the total variance represents "reproduction factor" and PC2 explaining 43.26 % variance representing "growth factor." Thus, the nature of macrophyte affects the growth and reproduction pattern of E. fetida among the different reactors, further the addition of A. pinnata in other macrophytes reactors can improve their recycling by E. fetida.

  9. Biodiversity and importance of floating weeds of Dara Ismail, Khan District of KPK, Pakistan.

    Science.gov (United States)

    Marwat, Sarfaraz Khan; Khan, Mir Ajab; Fazal-ur-Rehman; Ahmad, Mushtaq; Zafar, Muhammad

    2011-01-01

    The present paper is based on the results of taxonomic research work conducted in Dera Ismail Khan District of KPK, Pakistan, during 2005 - 2007. The area was extensively surveyed in order to collect floating aquatic weeds. From the study area 11 floating aquatic weed species belonging to 9 genera and 9 families were collected and identified in the light of available literature. These plants include Bryophytes: 1 species, Ricciocarpus natans (L.) Corda; Pteridophytes: 2 species, Azolla pinnata R.Br. and Marselia quadrifolia L., and Spermatophytes: 8 species, Lemna aequinoctialis Welw., L. gibba L., Marselia quadrifoliata L. Nelumbo nucifera Gaerth., Nymphoides cristata (Roxb.) O. Ketze. Nymphoides indica (L.) Kuntze:, Pistia stratiotes L. Potamogeton nodosus Poiret and Spirodela polyrrhiza (L.) Schleid. Floating weeds on one hand cause serious problems and on the other hand they are used for various purposes. Data inventory consists of botanical name, family, major group, habit and habitat, flowering period, availability, distribution in D.I.Khan, Pakistan and world, beneficial and harmful effects. Key to the floating aquatic species of the area was developed for easy and correct identification and differentiation.

  10. Effect of organic fertiliser residues from rice production on nitrogen fixation of soya (Glycine max L. Merrill, Chiang Mai 60 variety

    Directory of Open Access Journals (Sweden)

    Nattida Luangmaka

    2013-09-01

    Full Text Available A field study was undertaken on the residual effect of organic fertilisers applied to the preceding rice cropping on nitrogen fixation of soya in a rice-soya cropping system. The experiment was conducted on a farmer’s lowland paddy in Mae Rim district, Chiang Mai province, Thailand. Organic fertiliser treatments assigned were: 1 control (no fertiliser, 2 animal manure of cattle (AM, 3 compost (CP, 4 azolla (AZ, 5 AM + CP, 6 AM + AZ, 7 CP + AZ and 8 AM + CP + AZ. Soya seeds were planted without rhizobial inoculation in December 2011, four months after the application of organic fertilisers. Nodule weight, total shoot nitrogen accumulation and relative ureide index at various growth stages were recorded as the indices of nitrogen fixation. Results of the study demonstrate that the residues from the application the organic fertilisers of narrow C/N ratios during the land preparation for rice cropping four months before soya cultivation promoted nitrogen fixation by native rhizobia.

  11. Soils Newsletter. V. 12, no. 2

    International Nuclear Information System (INIS)

    This Newsletter includes reports of four research co-ordination meetings: on isotopic studies on nitrogen fixation and nitrogen cycling by blue-green algae and Azolla (final meeting, September 1989, IAEA, Vienna); on isotopic studies on increasing and stabilizing plant productivity in low phosphate and semi-arid and sub-humid soils of the tropics and sub-tropics (first meeting, October 1989, IAEA, Vienna); on the evaluation and calibration of nuclear techniques compared with traditional methods in soil water studies (July 1989, IAEA, Vienna); and on the use of isotopes in studies to improve the yield and nitrogen fixation of the common bean in Latin America (April 1989, Irapuato, Mexico). A new co-ordinated research programme on the use of nuclear and related techniques in the assessment of irrigation schedules of field crops to increase the effective use of water in irrigation projects is announced, and some of the technical co-operation programmes in the Latin America Region are briefly described. 2 tabs

  12. Aquatic Food Plants and their Consumer Birds at Sandi Bird Sanctuary, Hardoi, Northern India

    Directory of Open Access Journals (Sweden)

    Kaushalendra Kumar Jha

    2013-07-01

    Full Text Available One of the Bird Sanctuaries of Uttar Pradesh, Sandi, was selected for studying some ecological aspects like, aquatic food plants, their food calendar and dependent birds of migratory as well as resident origin. The study site is considered as an ideal wetland. This is located at 27o15’ N and 79o55’ E. Thirty four food plant species were identified to be eaten by 16 birds.These plants were the species of Alloteropsis, Arundo, Azolla, Ceratophyllum, Chloris, Commelina, Cyperus, Echinochloa, Eichhornia, Eleocharis, Hydrilla, Ipomoea, Jussiaea, Lemna, Najas, Nelumbo, Nymphea, Nymphoides, Oryza, Pistia, Polygonum, Potamogeton, Scirpus, Spirodela, Trapa, Typha, Vallisneria, and Wolffia. Common consumer birds eating plant parts were Coot, Pochards, Teal, Wigeon, Gadwal, Gargany, Goose, Whistling-duck, Mallard, Pintail, Shoveler, and Swamphen. These are primarily the migratory birds except Coot, Whistling-duck and Swamphen. Spot-billed Duck, and Indian Moorhen were occasionally seen eating submerged hydrophytes and filamentous slimy green algae. On the basis of multi-strata growth of plants in the Sanctuary a wetland profile was prepared. Food calendar i.e., availability of palatable parts of plants during different months was recorded. Information collected in the study could be used for habitat management, especially the weed removal and ensuring food sustainability for the vegetarian birds.

  13. 安徽省水生植物资源的调查与分析%Investigation and Conservation of Resources of Aquatic Higher Plants in Anhui

    Institute of Scientific and Technical Information of China (English)

    陈明林; 刘玲玲; 张小平

    2004-01-01

    本文研究了安徽省水生高等植物资源的种类、生境、组成特点、经济价值及植被概况,结果表明本省有水生高等植物44科、72属、138种,其中单子叶植物占优势,分别占科、属、种的40.9%、50.0%、54.4%.各科亲缘关系较远,并且许多科仅有一属的少数种为水生的.其地理成分表明,本地水生种子植物以世界分布属为主,热带成分与温带成分几乎各占一半,基本反映安徽省具有热带和温带的双重性质.水生植被以竹叶眼子菜(Potamogeton malaianus)群落,苦草(Vallisineria spiralis)群落,紫萍(Spirodela polyrrhiza)、浮萍(Lemna minor)群落,满江红(Azolla imbricata)、槐叶苹(Salvinia natans)群落,莲(Nelumbo nucifera)群落为主.

  14. Biodiversity and importance of floating weeds of Dara Ismail, Khan District of KPK, Pakistan.

    Science.gov (United States)

    Marwat, Sarfaraz Khan; Khan, Mir Ajab; Fazal-ur-Rehman; Ahmad, Mushtaq; Zafar, Muhammad

    2011-01-01

    The present paper is based on the results of taxonomic research work conducted in Dera Ismail Khan District of KPK, Pakistan, during 2005 - 2007. The area was extensively surveyed in order to collect floating aquatic weeds. From the study area 11 floating aquatic weed species belonging to 9 genera and 9 families were collected and identified in the light of available literature. These plants include Bryophytes: 1 species, Ricciocarpus natans (L.) Corda; Pteridophytes: 2 species, Azolla pinnata R.Br. and Marselia quadrifolia L., and Spermatophytes: 8 species, Lemna aequinoctialis Welw., L. gibba L., Marselia quadrifoliata L. Nelumbo nucifera Gaerth., Nymphoides cristata (Roxb.) O. Ketze. Nymphoides indica (L.) Kuntze:, Pistia stratiotes L. Potamogeton nodosus Poiret and Spirodela polyrrhiza (L.) Schleid. Floating weeds on one hand cause serious problems and on the other hand they are used for various purposes. Data inventory consists of botanical name, family, major group, habit and habitat, flowering period, availability, distribution in D.I.Khan, Pakistan and world, beneficial and harmful effects. Key to the floating aquatic species of the area was developed for easy and correct identification and differentiation. PMID:22754062

  15. Microrefugia and species persistence in the Galápagos highlands: a 26,000-year paleoecological perspective.

    Science.gov (United States)

    Collins, Aaron F; Bush, Mark B; Sachs, Julian P

    2013-01-01

    The Galápagos Islands are known to have experienced significant drought during the Quaternary. The loss of mesophytic upland habitats has been suggested to underlie the relatively lower endemism of upland compared with lowland plant assemblages. A fossil pollen record spanning the last 26,000 years from an upland bog on Santa Cruz Island, revealed the persistent presence of highland pollen and spore types during the last glacial maximum and a millennial-scale series of droughts in the mid Holocene. The absence of lowland taxa and presence of mesic taxa led to the conclusion that the highland flora of the Galápagos persisted during both these periods. The resiliency of the highland flora of the Galápagos to long-term drought contradicts an earlier hypothesis that an extinction of highland taxa occurred during the last glacial maximum and that rapid Holocene speciation created the modern plant assemblage within the last 10,000 years. Based on the palynological data, we suggest that, even during the height of glacial and Holocene droughts, cool sea-surface temperatures and strong trade-wind activity would have promoted persistent ground level cloudiness that provided the necessary moisture inputs to maintain microrefugia for mesophytic plants. Although moist conditions were maintained, the lack of precipitation caused the loss of open water habitat during such events, and accounts for the known extinctions of species such as Azolla sp., and Elatine sp., while other moisture dependent taxa, i.e., Cyathea weatherbyana, persisted.

  16. PARTIAL PURIFICATION AND CHARACTERIZATION OF LECTIN-LIKE ACTIVITIES FROM Lupinus albus SEEDS

    Directory of Open Access Journals (Sweden)

    A. B. Falcón

    2000-01-01

    Full Text Available En un trabajo anterior los autores estudiaron la actividad hemaglutinante presente en semillas de tres espe- cies de Lupinus; a partir de esos resultados se decidió purifi- car las actividades lectínicas presentes en semillas de Lupinus albus, tomando en cuenta las mayores especificidades encon- tradas previamente en los extractos. En este trabajo se prepa- raron tres columnas apropiadas de afinidad, con el fin de puri- ficar la actividad hemaglutinante de L. albus. El pellet colecta- do por centrifugación a partir del extracto previamente satura- do con 80 % de sulfato de amonio, fue aplicado respectivamante en tres columnas de afinidad: Mucina-Sepharosa, Fetuina- Sepharosa y Galactosa-Agarosa. Aunque fue purificada, a través de las columnas de Fetuina y Mucina, una actividad lectínica equivalente al 5 % aproximado del total de proteína encontra- do en el extracto, la actividad hemaglutinante pasó a través de la columna sin aparentemente interactuar con las proteínas fijadas en cada una de las columnas probadas. Las proteínas colectadas en los picos de elusión de las corridas cromatográficas fueron parcialmente caracterizadas mediante electroforesis en poliacrilamida y filtración en gel a través de una columna de Superosa 12, con el fin de conocer acerca de su composición monomérica y oligomérica y sus pesos moleculares. Se analizó, además, la posible estructura secundaria de la proteína a partir de un espectro de dicroismo circular realizado a la muestra.

  17. Flora y vegetación de suelos crioturbados y hábitats asociados en la Cordillera Blanca, Ancash, Perú

    Directory of Open Access Journals (Sweden)

    Asunción Cano

    2011-05-01

    Full Text Available Entre los años 2006 y 2008, se llevaron a cabo estudios florístico y de vegetación de los suelos crioturbados y hábitats asociados en cuatro localidades de la Cordillera Blanca (Ancash, Perú localizadas por encima de los 4500 m. Se realizaron recolectas botánicas además de transectos de intersección-línea, en los cuales se midió el espacio (en cm ocupado por cada especie. Fueron determinadas 136 especies de plantas vasculares, agrupadas en 65 géneros y 26 familias. Las Magnoliópsida (dicotiledóneas fueron las más diversas con 97 especies, seguidas de las Liliópsidas (Monocotiledóneas con 36. La mayor diversidad está concentrada en las familias Asteraceae y Poaceae (40,63%. Los géneros con mayor riqueza de especies fueron Senecio (18 y Calamagrostis (12. Se registraron 76 especies (54,82% en suelos crioturbados y hábitats asociados; mientras que 60 especies (44,11% fueron colectadas en la vegetación adyacente. El 95,56% de las especies reportadas fueron hierbas perennes. Se caracterizaron cuatro tipos de comunidades vegetales: a comunidad de suelos crioturbados propiamente dicha, b comunidad de suelos crioturbados asociada a pajonal, c comunidad de suelos crioturbados asociada a roquedal seco y d comunidad de suelos crioturbados asociadas a roquedal húmedo. Se indícan las especies características de cada comunidad.

  18. Concentración nutrimental en hojas de aguacate 'Hass' con síntoma de moteado Nutrimental concentration in 'Hass' avocado leaves with mottled symptom

    Directory of Open Access Journals (Sweden)

    Edgardo Federico Hernández-Valdés

    2012-06-01

    Full Text Available El llamado "moteado de las hojas" en el cultivo de aguacate está ampliamente distribuido en las zonas de producción más importante de México, y se desconoce los factores involucrados en la aparición de este síntoma foliar. Por ello, se contrastó el contenido nutrimental en hojas de aguacate 'Hass' colectadas de árboles sin la presencia del moteado (tratamiento A, contra hojas que tampoco manifestaran el síntoma pero provenientes de árboles con el daño foliar (tratamiento B y hojas con la presencia del síntoma (Tratamiento C. Se encontró que, aquellas hojas con "moteados" intervenales tuvieron menor concentración de N, P, K, y Mn, respecto a las que no presentaron el síntoma, mientras que las concentraciones de Ca, Mg, Fe, Zn, Cu y B hojas con y sin moteado fueron similares.The problem called as "mottled leaves" in avocado crop is widely spread in the most important production zones from Mexico, and it is unknown which factors are involved in this foliar symptom appearance. By this reason, the nutrimental content in 'Hass' avocado foils collected from trees without mottled presence (treatment A was collated against leaves that either showed the symptom although coming from trees with foliar damage (treatment b and leaves with symptom presence (Treatment C. It was found that those leaves with interveinal "mottled" had least N, P, K, and Mn concentration with regards the ones that show no symptom, while Ca, Mg, Fe, Zn, Cu and B concentrations in leaves with and without mottling were similar.

  19. Visitantes florales nocturnos del girasol (Helianthus annuus, Asterales: Asteraceae en la Argentina

    Directory of Open Access Journals (Sweden)

    Juan P TORRETTA

    2009-01-01

    Full Text Available El girasol (Helianthus annuus es un cultivo oleaginoso, polinizado por Apis mellifera L. y otras abejas en distintas regiones del mundo. Sin embargo, sus flores también son visitadas por insectos de actividad nocturna. Durante tres campañas agrícolas, se determinó la diversidad de los visitantes nocturnos de capítulos de girasol, en cinco sitios de Argentina. También se estudió el comportamiento de forrajeo de los principales visitantes y la variación de la receptividad estigmática a lo largo del día, con el fin de establecer si estos visitantes contribuyen a la polinización. Al menos 67 especies o morfoespecies pertenecientes a cuatro órdenes de visitantes nocturnos fueron colectadas. El orden más rico y abundante fue Lepidoptera (44 especies o morfoespecies, cinco familias, seguido por Coleoptera (18 especies o morfoespecies, nueve familias, Orthoptera (tres morfoespecies, una familia y Blattaria (dos especies, una familia. Los lepidópteros forrajearon exclusivamente por néctar, mientras que los individuos de los demás órdenes consumieron polen y/o partes florales. El estigma se encontró receptivo durante las horas de luz, con una receptividad máxima al mediodía (12:00 - 14:00. Llamativamente, las flores del girasol son visitadas por mayor número de polillas que de abejas. Debido a que las polillas consumen néctar y potencialmente transportan polen entre flores, en un momento del día en que los estigmas se encuentran menos receptivos, es improbable que polinicen efectivamente el cultivo.

  20. CONDICIONES DE CULTIVO DE LA PLANTA MATRIZ Y USO DEL ÁCIDO INDOLBUTÍRICO EN LA PROPAGACIÓN DEL MANDARINO ‘SUNKI’ BAJO ESTAQUILLA

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    ANDRÉS IVÁN PRATO SARMIENTO

    2016-01-01

    Full Text Available RESUMEN Los patrones se obtienen tradicionalmente en la citricultura a partir de semillas, seleccionándose las plantas de origen apomíctica. Sin embargo, hay riesgo de no realizarse la selección correcta, debido a la baja apomixis y la alta heterocigocidad en algunas especies o cultivares, como el mandarino ‘Sunki’. La obtención de los mismos por estaquillas solucionaría el problema. El objetivo de este trabajo fue evaluar el potencial de enraizamiento de estaquillas del mandarino ‘Sunki’ (Citrus sunki Hort. ex Tan., colectadas de matrices cultivadas en el campo o en ambiente protegido, utilizándose diferentes concentraciones del ácido indolbutírico (AIB. Las estaquillas se colectaron en el final de la primavera y se mantuvieron por 90 días en cámara de nebulización intermitente. Se adoptó el diseño experimental de bloques completos al azar, con cuatro repeticiones, en arreglo factorial 2 x 4, referente a dos condiciones de cultivo (matrices cultivadas en campo y en ambiente protegido y cuatro concentraciones del AIB (0, 750, 1.500 y 3.000 mg L-1. El enraizamiento del mandarino ‘Sunki’ fue elevado, alcanzando el 96% en las dos condiciones. La brotación (54% y la supervivencia (95% de las estacas presentaron valores satisfactorios, independientemente del AIB. El empleo del AIB solamente es necesario en estacas provenientes de plantas mantenidas en el ambiente protegido, puesto que mejora su enraizamiento y el número de raíces.