Sample records for azines

  1. Expression of ODC Antizyme Inhibitor 2 (AZIN2 in Human Secretory Cells and Tissues.

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    Tiina Rasila

    Full Text Available Ornithine decarboxylase (ODC antizyme inhibitor 2 (AZIN2, originally called ODCp, is a regulator of polyamine synthesis that we originally identified and cloned. High expression of ODCp mRNA was found in brain and testis. We reported that AZIN2 is involved in regulation of cellular vesicle transport and / or secretion, but the ultimate physiological role(s of AZIN2 is still poorly understood. In this study we used a peptide antibody (K3 to human AZIN2 and by immunohistochemistry mapped its expression in various normal tissues. We found high expression in the nervous system, in type 2 pneumocytes in the lung, in megakaryocytes, in gastric parietal cells co-localized with H,K-ATPase beta subunit, in selected enteroendocrine cells, in acinar cells of sweat glands, in podocytes, in macula densa cells and epithelium of collecting ducts in the kidney. The high expression of AZIN2 in various cells with secretory or vesicle transport activity indicates that the polyamine metabolism regulated by AZIN2 is more significantly involved in these events than previously appreciated.

  2. Synthesis and mesomorphic properties of new azine-type liquid crystals

    Institute of Scientific and Technical Information of China (English)

    Qiang Wei; Lin Shi; Hui Cao; Huai Yang; Yan Bin Wang


    A series of symmetrical azine-type liquid crystals were synthesized. The characteristic of these liquid crystals is that they had high clearing point (~320 ℃) and broad thermal range of nematic phase (~154 ℃). It was also found that the end groups of the liquid crystals had effect on the mesomorphic properties.

  3. Tuning interaction in dinuclear ruthenium complexes : HOMO versus LUMO mediated superexchange through azole and azine bridges

    NARCIS (Netherlands)

    Browne, Wesley; Hage, R; Vos, Johannes G.


    In this review the interaction between metal centers in dinuclear complexes based on azole and azine containing bridging ligands is reviewed. The focus of the review is on the manner in which the interaction pathway can be manipulated by variations in the nature of both the direct bridging unit and

  4. Applications of and alternatives to pi-electron-deficient azine organometallics in metal catalyzed cross-coupling reactions. (United States)

    Campeau, Louis-Charles; Fagnou, Keith


    While the use of pi-deficient azine halides in palladium catalyzed cross-coupling reactions is common, the use of pi-electron deficient azine organometallics has been less intensively examined. In recent years, important advances have been made that are beginning to address this deficiency and need. The purpose of this tutorial review is to highlight and discuss the innovations that facilitate the synthesis of azine-containing biaryls with a focus on the pyridine structural motif. Given the number of important compounds which exhibit azine-heterobiaryls and the wide use of cross-coupling methods in their synthesis, this review should be of interest among synthetic organic chemists and organometallic chemists alike.

  5. Synthesis and characterizaton of some new coordination compounds of boron with mixed azines

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    Full Text Available Some new boron complexes have been synthesized by the reaction of triisopropohxyborane with the mixed azines, prepared by the condensation of salicylaldehyde and hydrazine with aldehydes/ketones in a 1:1:1 mole ratio to give a new series of (OPri2B(NO type of complexes. Their structures were confirmed on the basis of elemental analyses, ultraviolet, infrared, 1H-NMR and 11B-NMR spectral studies. The ligands and their boron complexes were also screened for their antifungal activity. Several of these complexes were found to be quite active in this respect.

  6. The electrochemical reduction of 1,4-dichloroazoethanes: Reductive elimination of chloride to form aryl azines

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    Sauro, Vittorio A.; Magri, David C.; Pitters, Jason L. [Department of Chemistry, University of Western Ontario, London, ON, N6A 5B7 (Canada); Workentin, Mark S., E-mail: mworkent@uwo.c [Department of Chemistry, University of Western Ontario, London, ON, N6A 5B7 (Canada)


    Graphical abstract: -- Abstract: A series of 1,4-dichloroazoethanes (1-X/Y, X and Y = 4-NO{sub 2}, 4-CN, 4-CH{sub 3} or 4-H) were studied in N,N-dimethylformamide using cyclic voltammetry, constant potential sweep voltammetry (CPSW) and constant potential electrolysis. The voltammograms of 1-X/Y exhibit an irreversible two-electron wave corresponding to dissociative electron transfer (DET) reduction of the carbon-chlorine bond resulting in formation of the azines 2-X/Y in quantitative yield. Additional redox waves correspond to the reversible reduction of the azines to the 2-X/Y{center_dot}{sup -} radical anion and 2-X/Y{sup 2-} dianion consecutively, with the exception of 1-NO{sub 2}/NO{sub 2} where both NO{sub 2} groups are reduced simultaneously in a two-electron reversible wave. Thermodynamic and kinetic parameters were determined from CPSW: the standard reduction potentials (E{sup o}) vary between -0.7 and -1.3 V versus SCE as a function of electron-withdrawing substituent; the heterogeneous rate constants (k{sub het}) are consistent with a slow heterogeneous electron transfer with values ranging from 10{sup -3} to 10{sup -5} cm s{sup -1}; the transfer coefficients ({alpha}) for 1-NO{sub 2}/NO{sub 2} and 1-NO{sub 2}/H are greater than 0.5, indicative of a stepwise DET mechanism for the C-Cl bond cleavage while the remaining 1-X/Y compounds have {alpha} values between 0.35 and 0.5, and the intrinsic barriers are all significantly lower than predicted for a concerted DET, thereby also suggesting a stepwise DET mechanism.

  7. Expression of ODC1, SPD, SPM and AZIN1 in the hypothalamus, ovary and uterus during rat estrous cycle. (United States)

    Fernandes, Joseph R D; Jain, Sammit; Banerjee, Arnab


    The aim of the present study was to investigate variation in the expression pattern of ornithine decarboxylase (ODC1), spermine (SPM), spermidine (SPD) and antizyme inhibitor (AZIN1) in hypothalamus, ovary and uterus during the estrous cycle of rats. Further, to understand any correlation between polyamines and GnRH I expression in hypothalamus; effect of putrescine treatment on GnRH I expression in hypothalamus and progesterone and estradiol levels in serum were investigated. The study also aims in quantifying all the immunohistochemistry images obtained based on pixel counting algorithm to yield the relative pixel count. This algorithm uses a red green blue (RGB) colour thresholding approach to quantify the intensity of the chromogen present. The result of the present study demonstrates almost similar expression pattern of polyamine and polyamine related factors, ODC1, SPD, SPM and AZIN1, with that of hypothalamic GnRH I, all of which mainly localized in the medial preoptic area (MPA) of the hypothalamus, during the proestrus, estrus and diestrus. This suggest that hypothalamic GnRH I expression is under regulation of polyamines. The study showed significant increase in hypothalamic GnRH I expression for both the doses of putrescine treatment to adult female rats. Further, it was shown that in ovary expression pattern of ODC1, SPM, SPD and AZIN1 were similar with that of steroidogenic factor, StAR during the estrous cycle, and putrescine supplementation increased significantly estradiol and progesterone levels in serum, all suggesting ovarian polyamines are involved in regulation of ovarian steroidogenesis. Localization of these factors in the theca and granulosa cells suggest involvement of polyamines in the process of folliculogenesis and luteinization; and ODC1, SPD, SPM and AZIN1 in oocyte further suggests polyamine role in maintenance of oocyte physiology. Finally, in uterus SPM and AZIN1 were localized throughout the estrous cycle, being comparatively more

  8. An Azine-Linked Covalent Organic Framework: Synthesis, Characterization and Efficient Gas Storage. (United States)

    Li, Zhongping; Zhi, Yongfeng; Feng, Xiao; Ding, Xuesong; Zou, Yongcun; Liu, Xiaoming; Mu, Ying


    A azine-linked covalent organic framework, COF-JLU2, was designed and synthesized by condensation of hydrazine hydrate and 1,3,5-triformylphloroglucinol under solvothermal conditions for the first time. The new covalent organic framework material combines permanent micropores, high crystallinity, good thermal and chemical stability, and abundant heteroatom activated sites in the skeleton. COF-JLU2 possesses a moderate BET surface area of over 410 m(2)  g(-1) with a pore volume of 0.56 cm(3)  g(-1) . Specifically, COF-JLU2 displays remarkable carbon dioxide uptake (up to 217 mg g(-1) ) and methane uptake (38 mg g(-1) ) at 273 K and 1 bar, as well as high CO2 /N2 (77) selectivity. Furthermore, we further highlight that it exhibits a higher hydrogen storage capacity (16 mg g(-1) ) than those of reported COFs at 77 K and 1 bar.

  9. Long-living structures of photochromic salicylaldehyde azine: polarity and viscosity effects from nanoseconds to hours. (United States)

    Ziółek, Marcin; Burdziński, Gotard; Douhal, Abderrazzak


    In this study, we report on the effects of solvent viscosity and polarity on the photochromic salicylaldehyde azine (SAA) molecule by examining the steady-state and UV-visible absorption results in the time scale from nanoseconds to hours, in solution and in a polymer film. For the neutral structure, the viscosity strongly affects the lifetime of the photochromic (trans-keto) tautomer by suppressing the second order quenching process, and thus increasing the photochrome lifetimes in highly viscous solvents to 500 μs in polar triacetine, and to 65 μs in non-polar squalane. Trapping SAA in a non-polar polymer film (polyethylene) results in further elongation of the photochromic lifetime (700 μs) by one order of magnitude (with respect to that in squalane), due to the retardation of the intramolecular back-isomerization. Another species, living significantly longer and absorbing more in the UV comparing to the photochrome, was identified as the syn-enol tautomer. The lifetime of this tautomer, created in a competitive mechanism to the photochrome creation, is much longer in non-polar solvents (hundreds of minutes) than in polar ones (tens of minutes), opposite to the trend observed for the photochrome. For the SAA anion, the transient living on the ns-μs time scale can be exclusively assigned to the triplet state, which is not observed for the neutral form at room temperature.

  10. Tetrathiafulvalene-based azine ligands for anion and metal cation coordination

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    Awatef Ayadi


    Full Text Available The synthesis and full characterization of two tetrathiafulvalene-appended azine ligands, namely 2-([2,2’-bi(1,3-dithiolylidene]-4-yl-6-((2,4-dinitrophenylhydrazonomethylpyridine (L1 and 5-([2,2’-bi(1,3-dithiolylidene]-4-yl-2-((2,4-dinitrophenylhydrazonomethylpyridine (L2 are described. The crystal structure of ligand L1 indicates that the ligand is completely planar with the presence of a strong intramolecular N3–H3···O1 hydrogen bonding. Titration experiments with inorganic anions showed that both ligands are suitable candidates for the sensing of fluoride anions. Ligand L2 was reacted with a Re(I cation to yield the corresponding rhenium tricarbonyl complex 3. In the crystal structure of the newly prepared electroactive rhenium complex the TTF is neutral and the rhenium cation is hexacoordinated. The electrochemical behavior of the three compounds indicates that they are promising for the construction of crystalline radical cation salts.

  11. Synthesis of Symmetrical and Asymmetrical Azines Encompassing Naphtho[2,1-b]furan by a Novel Approach

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    K. Veena


    Full Text Available The starting material 3-nitro-2-acetylnaphtho[2,1-b]furan (2 was obtained by nitration of 2-acetylnaphtho[2,1-b] furan (1, under mild condition. The compound 1 was synthesized by the reaction of 2-hydroxy-1-naphthaldehyde with chloroacetone, where in both condensation and cyclization took place in single step. The reaction of 3-nitro-2-acetylnaphtho[2,1-b]furan (2 with hydrazine hydrate produced corresponding hydrazone (3 in excellent yield, which on treatment with various aromatic aldehydes under different reaction conditions resulted in the formation of symmetrical azines (4a-e and unsymmetrical azines (5a-e. All the newly synthesized compounds have been characterized by analytical and spectral studies and were screened for antibacterial antibacterial activity against Bacillus subtilus and Alcaligenes fecalies and antifungal activity against Aspergillus nidulans, Aspergillus parasiticus and Aspergillus terrus. The Second Harmonic Generation (SHG efficiency of some of the synthesized compounds was measured by powder technique using Nd:YAG laser.

  12. Transition Metal Complexes of 1, 4(2'-Hydroxyphenyl-1-yl di-imino azine: Synthesis, Characterization and Antimicrobial Studies

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    M. Revanasiddappa


    Full Text Available The synthesis and characterization of first row transition metal complexes of the 1, 4(2'-hydroxyphenyl-1-yl di-imino azine {1,4(2'HPDA} are reported. The complexes have been characterized by elemental analysis, molar conductance, magnetic studies, IR, 1H NMR and UV-visible studies. They have the stoichiometry of the type [M{1,4(2'HPDA}2 2H2O ] and [M'L2] where M= Mn(II, Fe(III, Co(II, Ni(II and Cu(II, and M' = ZrO(II, VO(II, Zn(II, Cd(II, and Hg(II. The antibacterial and antifungal activity of the metal complexes has been investigated. Both ligand and complexes have shown good antibacterial and antifungal activity.

  13. Estrogenic effects of the new opioid antagonist naltrexone-estrone azine on pituitary luteinizing hormone secretion in ovariectomized rats. (United States)

    Armeanu, M C; van Dieten, J A; Kolb, V M; Schoemaker, J; de Koning, J


    The effect of the new opioid antagonist naltrexone-estrone azine (EH-NX) on pituitary luteinizing hormone (LH) secretion in the ovariectomized rat was studied. EH-NX is a hybrid between the steroid component estrone and the opioid antagonist naltrexone (NX). It is a potent and long-acting opioid antagonist in vitro and in vivo, but its effect upon in vivo LH secretion has not been tested before. The aims of the study were to investigate whether, unlike naltrexone, EH-NX can stimulate LH secretion without the need of additional estrogen pretreatment and whether EH-NX has peripheral estrogenic effects upon the uterine weight, when administered chronically to long-term ovariectomized rats. Female rats were injected subcutaneously with EH-NX 21 days after ovariectomy. The effects of EH-NX injections on LH secretion were compared to the effects of NX and estrone hydrazone (EH) alone, or in combination, with or without estradiol-benzoate (EB) pretreatment. Inhibition of LH secretion and uterine proliferation were observed in rats treated chronically with EH-NX in dosages of 0.250 mg/kg bw and higher. These effects were similar to those caused by EH and EB. In short-term OVX rats EH-NX appeared to act faster than EH. In contrast to NX, no stimulatory effect on LH secretion was seen with EH-NX in EB primed OVX rats. These results surprisingly demonstrate that EH-NX behaves like an estrogen and not like an opioid antagonist. The unexpected pharmacological profile of this new drug may open up doors for several medical applications.

  14. Crystal structures and spectral properties of two polyoxometalate-based inorganic-organic compounds from silver-azine building blocks with bis-bidentate and tridentate ligands (United States)

    An, Bing; Zhou, Rui-Min; Sun, Li; Bai, Yan; Dang, Dong-Bin


    Two polyoxometalate-based inorganic-organic hybrid compounds constructed from silver(I)-L species and saturated Keggin type polyoxoanion, [Ag2L21]2(SiMo12O40)·1.5DMF·0.5CH3OHṡH2O 1 and [{Ag4L22(DMF)5}(SiMo12O40)] 2 (L1 = phenyl 2-pyridyl ketone azine, L2 = 3-phenyltriazolo[1,5-a]pyridine), have been synthesized and structurally characterized by IR, UV, elemental analysis, XRPD and complete single crystal structure analyses, where the ligands L1 and L2 are bis-bidentate and tridentate azines synthesized with the same materials under different conditions. The structure of 1 exhibits a dinuclear double-helicate with [SiMo12O40]4- anions as counter ions, where all of the Ag centers coordinate to bis-bidentate chelating ligands. Compound 2 displays a two-dimensional sheet formed by the Ag-organic infinite chains and the [SiMo12O40]4- alternately arranged in a “rail-like” fashion. The luminescent properties of 1 and 2 in the solid state were investigated.

  15. A highly selective colorimetric and "turn-on" fluorimetric chemosensor for detecting CN- based on unsymmetrical azine derivatives in aqueous media (United States)

    Sun, You; Hu, Jing-Han; Qi, Jing; Li, Jian-Bin


    A novel highly selective chemosensor S1 for cyanide based on unsymmetrical azine derivative was successfully designed and synthesized, which showed both colorimetric and fluorescence turn-on responses for cyanide ions in aqueous. This structurally simple chemosensor could detect CN- anion over other anions in aqueous solution DMSO/H2O (v/v = 3:2) undergo deprotonation reaction. Results showed that the chemosensor S1 exhibited 50 fold enhancement in fluorescence at 530 nm and showed an obvious change in color from colorless to yellow that could be detected by naked eye under the UV-lamp after the addition of CN- in aqueous solution. Moreover, the detection limit on fluorescence response of the sensor to CN- is down to 6.17 × 10- 8 M by titration method. Test strips based on S1 were obtain, which could be used as a convenient and efficient CN- test kit to detect CN- in aqueous solution.

  16. 丙酮连氮催化水解反应精馏工艺的研究%Process of hydrazine hydrate production by acetone azine hydrolysis via catalytic distillation

    Institute of Scientific and Technical Information of China (English)

    李柏春; 吴晓旺; 许沉娜


    The process of hydrazine hydrate produced by acetone azine hydrolysis via catalytic distillation was simulated and experimented.By using NTRL equation and RadFrac module,as well as the data obtained from dynamics and phase equilibrium,the process of producing hydrazine hydrate by acetone azine hydrolysis via catalytic distillation was simulated.The effects of process parameter,such as reflux ratio,mole ratio of acetone azine to water and feeding location on the acetone azine hydrolysis reaction were investigated.The optimized process parameters obtained were as follows:theoretical tray number 5 for rectifying,theoretical tray number 14 for reaction,reflux ratio 2,the mole fraction of acetone azine to water 1:7,feed tray 9th tray.Under such conditions,the hydrolysis rate of acetone azine was over 99%,the mass fraction of hydrazine hydrate at bottom was 34% and the temperature and mass fraction distributions of whole column were got.It provides a reference for industry production.%对丙酮连氮催化水解反应精馏制水合肼的工艺过程进行了模拟与实验.采用NRTL方程与RadFrac模型,使用实验回归得到的动力学方程与汽液平衡参数,对丙酮连氮催化水解精馏制水合肼的工艺进行模拟计算.考察了回流比、丙酮连氮与水的摩尔比、进料位置等工艺条件对丙酮连氮水解反应的影响,并通过实验验证了模型的准确性和可靠性.在模拟和实验的基础上,确定了最优工艺条件:精馏段理论板数为5,反应精馏段理论板数为14,回流比为2,丙酮连氮与水的摩尔比为1:7,原料进料位置为第9块板.在此条件下,丙酮连氮的水解率可达99%以上,塔釜肼质量分数为34%,并且得到了全塔的温度和质量分数分布曲线,为工业生产提供了依据.

  17. 丙酮-水-丙酮连氮体系汽液平衡测定与关联%Measurement and correlation of vapor-liquid equilibria for acetone-water-azine system

    Institute of Scientific and Technical Information of China (English)

    李柏春; 王琮; 黄岩; 张文林


    Vapor-liquid equilibrium (VLE) data for acetone-azine binary system and parts of acetone water azine ternary system were determined in an Othmer equilibrium at atmospheric pressure (101.3 kPa). The experimental data was verified by Herington method and were consistent with thermodynamics. The data of acetone-azine binary system were correlated with the NRTL,Wilson and UNIQUAC models by using chemical engineering analogue software. Respectively,all the models were correlated well with the VLE data. NRTL model produced best data,with the average deviations of temperature of 0.0639 K and vapor-phase mole fraction of methanol of 0.0048. The NRTL model was used to correlate the VLE data of the ternary system,and the interaction parameters of acetone-azine,water-azine,and acetone-water were obtained. In order to verify the reliability of the VLE data of the ternary system and these interacting binary parameters,the vapor compositions were calculated and compared with the corresponding experimental VLE data of acetone-azine binary system. The agreement between the calculated and experimental data was satisfactory. The experimental and correlative data could provide a basis for distillation separation of acetone-water-azine’s VLE system.%采用汽液双循环平衡釜,在常压(101.3 kPa)下测定了丙酮-丙酮连氮二元组分物系以及部分丙酮-水-丙酮连氮三元组分物系的汽液平衡数据。实验数据经Herington面积积分法检验符合热力学一致性。通过化工过程模拟软件,分别采用 Wilson、NRTL、UNIQUAC 活度系数模型对丙酮-丙酮连氮二元组分物系实验数据进行关联得到模型参数。并对汽液平衡的计算值与实验值进行比较,3种模型都吻合良好,其中由NRTL方程关联得到的计算结果最为适合,平均温度偏差和汽相组成偏差分别为0.0639 K和0.0048。从三元汽液相平衡数据中拟合出了丙酮-丙酮连氮、丙酮-

  18. Synthesis、Crystal Structure and Optical Properties of O-vanillin Azine%联氮香兰素的合成、晶体结构和光学性质

    Institute of Scientific and Technical Information of China (English)

    田玉鹏; 赵祖志; 周虹屏


    The title compound, o-vanillin azine, has been prepared by the reaction of o-vanillin with 0. 5 equivof hydrated hydrazine in ethanol. The new compound (C16H16N2O4, Mr = 300. 31) has been determined by singlecrystal X-ray diffraction method. The crystal belongs to monoclinic, space group P21/n with a = 5. 988 3(6), b =18. 654(2), c = 0. 686 75(7) nm, β = 106. 42(1)°, V= 0. 735 84(13) nm 3, Z = 2, Dc = 1. 355 g/cm3, F(000) = 316 andμ(MoKa) = 0. 099 mm=1 (λ= 0. 071 073 nm), R = 0. 073 6 and wR = 0. 159 5 for 1 292 observed reflections (I > 2σ( I )).%利用在甲醇中香兰素和肼反应合成了目标化合物-联氮香兰素.用单晶X-射线衍射法测定了化合物晶体结构,结果表明化合物晶体为单斜晶系,P21/n空间群,a=0.598 83(6),b=1.865 4(2),c=0.686 75(7)nm,β=106.42(1)°,V=0.73584(13)nm3,Z=2,Dc=1.355 g/cm3,F(000)=316,μ(MoKa)=0.099 mm-1.可观测衍射点1 292个,最终限制因子R=0.0736,ωR=0.159 5.

  19. 铜离子催化DPKA关环反应及其潜在应用%Cu Ⅱ-induced Ring-formation Reaction of Di-pyridylketone Azine and Its Potential Application

    Institute of Scientific and Technical Information of China (English)

    吴大雨; 黄薇; 闫文波; 段春迎; 谢黎霞; 孟庆金


    铜离子催化的氧化关环有机反应,用于选择性荧光探测金属铜离子的存在.晶体结构和光谱分析表明,在DPKA的乙腈/水混合溶剂中,引入铜离子可以促使苯并三氮唑类荧光化合物(2)的形成,该反应可以使得溶液的荧光明显增强.而且在滴定溶液里,引入Cu2+螯合剂EDTA,并不能使得溶液的荧光减弱,表明荧光是由有机物2发射的.同时,其他金属离子的引入并不能产生荧光,而且干扰离子的存在也不能使得荧光光谱发生显著改变,表明DPKA可以高选择性的检测溶液中的铜离子.研究表明,荧光响应呈现2个线性关系,区间分别在0.64~3.2 mg·kg-1和3.84~7.04 mg·kg-1.%A Cu-promoted ring-closed reaction of di-pyridylketone azine (DPKA) was developed in the design of a fluorescent chemodosimeter for potential selective Cu (Ⅱ)detection. Structure and spectroscopic investigations demonstrated the formation of a 3H-pyrazolo[4,3-b]pyridine (2) and the "turn-on" response for Cu(Ⅱ) in aqueous acetonitrile media. The introduction of Cu2+ chelator, such as EDTA, into the titration solution will not weaken the fluorescence, indicating the fluorescence is emitted from the organic compound (2). Two independent linear correlations exist in the range of 0.64~3.2mg·kg-1 and 3.84~7.04mg·kg-1,respectively.

  20. The Electrochemistry of Azine, Oxazine and Thiazine Dyes (United States)


    Electroanalytical Chemistry , 152, (1983), 197-209... Electroanalytical Chemistry , to be submitted. 6. "Transient Photochemistry of Methylene Blue in DMSO", E. D. Lee, D. A. Aikens, and H. H. Richtol, Journal of... Electroanalytical Chemistry , to be submitted. 8. "Linear Sweep Voltammetry of Thionine and Methylene Blue in DMSO", J. Fitzsimmons, D. A. Aikens, and H. H. Richtol, Journal of Electroanalytical Chemistry , to be submitted. 40 01

  1. Computational analysis of azine-N-oxides as energetic materials

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    Ritchie, J.P.


    A BKW equation of state in a 1-dimensional hydrodynamic simulation of the cylinder test can be used to estimate the performance of explosives. Using this approach, the novel explosive 1,4-diamino-2,3,5,6-tetrazine-2,5-dioxide (TZX) was analyzed. Despite a high detonation velocity and a predicted CJ pressure comparable to that of RDX, TZX performs relatively poorly in the cylinder test. Theoretical and computational analysis shows this to be the result of a low heat of detonation. A conceptual strategy is proposed to remedy this problem. In order to predict the required heats of formation, new ab initio group equivalents were developed. Crystal structure calculations are also described that show hydrogen-bonding is important in determining the density of TZX and related compounds.

  2. Some azine and azole derivatives as antioxidant additives for lithium lubricating grease

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    Reda M. Fikry


    The structure of these compounds has been confirmed using elemental analysis, nuclear magnetic resonance and infrared spectroscopy. On the other hand, lithium lubricating grease was prepared and evaluated according to the National Lubricating Grease Institute (NLGI and Egyptian Standard (ES. The antioxidant compounds were tested for the prepared grease with concentration 0.1 wt.%. The efficiency of these compounds has been determined using total acid number. It was found that the prepared lithium grease resists oxidation reaction in the presence of these compounds. A correlation between the chemical structure of these compounds and their efficiency as antioxidants additives was discussed. Data showed that the efficiency of these compounds as antioxidants decreases in the order: compound I > compound II > compound III.

  3. Structural and degradative aspects of ornithine decarboxylase antizyme inhibitor 2

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    Bruno Ramos-Molina


    Full Text Available Ornithine decarboxylase (ODC is the key enzyme in the polyamine biosynthetic pathway. ODC levels are controlled by polyamines through the induction of antizymes (AZs, small proteins that inhibit ODC and target it to proteasomal degradation without ubiquitination. Antizyme inhibitors (AZIN1 and AZIN2 are proteins homologous to ODC that bind to AZs and counteract their negative effect on ODC. Whereas ODC and AZIN1 are well-characterized proteins, little is known on the structure and stability of AZIN2, the lastly discovered member of this regulatory circuit. In this work we first analyzed structural aspects of AZIN2 by combining biochemical and computational approaches. We demonstrated that AZIN2, in contrast to ODC, does not form homodimers, although the predicted tertiary structure of the AZIN2 monomer was similar to that of ODC. Furthermore, we identified conserved residues in the antizyme-binding element, whose substitution drastically affected the capacity of AZIN2 to bind AZ1. On the other hand, we also found that AZIN2 is much more labile than ODC, but it is highly stabilized by its binding to AZs. Interestingly, the administration of the proteasome inhibitor MG132 caused differential effects on the three AZ-binding proteins, having no effect on ODC, preventing the degradation of AZIN1, but unexpectedly increasing the degradation of AZIN2. Inhibitors of the lysosomal function partially prevented the effect of MG132 on AZIN2. These results suggest that the degradation of AZIN2 could be also mediated by an alternative route to that of proteasome. These findings provide new relevant information on this unique regulatory mechanism of polyamine metabolism.

  4. Ornithine decarboxylase antizyme inhibitor 2 regulates intracellular vesicle trafficking

    Energy Technology Data Exchange (ETDEWEB)

    Kanerva, Kristiina; Maekitie, Laura T. [Department of Pathology, Haartman Institute, University of Helsinki, Helsinki (Finland); Baeck, Nils [Department of Anatomy, Institute of Biomedicine, University of Helsinki, Helsinki (Finland); Andersson, Leif C., E-mail: [Department of Pathology, Haartman Institute, University of Helsinki, Helsinki (Finland); HUSLAB, Helsinki (Finland); Department of Oncology and Pathology, Karolinska Institutet, Stockholm (Sweden)


    Antizyme inhibitor 1 (AZIN1) and 2 (AZIN2) are proteins that activate ornithine decarboxylase (ODC), the key enzyme of polyamine biosynthesis. Both AZINs release ODC from its inactive complex with antizyme (AZ), leading to formation of the catalytically active ODC. The ubiquitously expressed AZIN1 is involved in cell proliferation and transformation whereas the role of the recently found AZIN2 in cellular functions is unknown. Here we report the intracellular localization of AZIN2 and present novel evidence indicating that it acts as a regulator of vesicle trafficking. We used immunostaining to demonstrate that both endogenous and FLAG-tagged AZIN2 localize to post-Golgi vesicles of the secretory pathway. Immuno-electron microscopy revealed that the vesicles associate mainly with the trans-Golgi network (TGN). RNAi-mediated knockdown of AZIN2 or depletion of cellular polyamines caused selective fragmentation of the TGN and retarded the exocytotic release of vesicular stomatitis virus glycoprotein. Exogenous addition of polyamines normalized the morphological changes and reversed the inhibition of protein secretion. Our findings demonstrate that AZIN2 regulates the transport of secretory vesicles by locally activating ODC and polyamine biosynthesis.

  5. Compound list: promethazine [Open TG-GATEs

    Lifescience Database Archive (English)

    Full Text Available promethazine PMZ 00110 ...

  6. International Conference on Numerical Grid Generation in Computational Fluid Dynamics (United States)


    Grun Convex Computer Corporation Brunnenstr. 17 701 Piano Road 8049 Bachenhausen Richardson Germany TX 75081 Chunyuan Gu J. E.Holcomb Dept. of Gas...Lab System Dynamics Inc. L-95, PO Box 808 1211 N.W. 10th Avenue Livermore, CA 94550 Gainesville FL 32601 Bernadette Palmero Azine Renzo Universite de

  7. Speech Recognition Using Neural Nets and Dynamic Time Warping (United States)


    8217 Phonetic Typewriter", Computer, 21: 11-22 (March 1988). - - 7. Lippmann, Richard P. "An Introduction to Computing with Neural Nets," IEEE ASSP Mag...azine, 4: 4-22 (April 1987). 8. Kohonen, Teuvo and others. "Phonotopic Maps-Insightful Representation of Phonological Features for Speech Recognition

  8. Synthesis of -C[double bond, length as m-dash]N- linked covalent organic frameworks via the direct condensation of acetals and amines. (United States)

    Li, Zhi-Jun; Ding, San-Yuan; Xue, Hua-Dong; Cao, Wei; Wang, Wei


    We demonstrate herein a facile approach for constructing -C[double bond, length as m-dash]N- linked COFs from acetals. Three new COFs (imine-linked LZU-20, hydrazone-linked LZU-21, and azine-linked LZU-22) were synthesized by the direct condensation of dimethyl acetals and amines. All the synthesized COFs are highly crystalline and exhibit good thermal stability.

  9. 3-hydroxypyridazine 1-oxides as carboxylate bioisosteres

    DEFF Research Database (Denmark)

    Greenwood, Jeremy R; Mewett, Ken N; Allan, Robin D;


    /2 over GluR3/4 is the highest yet observed among azine-based glutamate analogues. The non-isosteric congener 2 showed weaker binding to AMPA-Rs. In the cortical wedge, 1 evokes similar responses to AMPA, while 3 and 2 are 10- and 100-fold weaker, respectively. Dose-response curves on Xenopus laevis...

  10. Drug: D00480 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available fecting cellular function 44 Allergic agents 441 Antihistamines 4413 Phenothiazin... ANTIHISTAMINES, ANESTHETICS, ETC. D04AA Antihistamines for topical use D04AA10 P...azine D00480 Promethazine hydrochloride (JP16/USP) Respiratory Tract/Pulmonary Agents Antihistamine

  11. Chemistry of the Burning Surface (United States)


    control voltage of the Pt filament is highly terial. This chemistry cannot be extracted by slowly heat- sensitive to the thermochemistry of the thin...azines. the residues formed at teach iernperature ’)ascd ýucli as melon, arc known to be thcriaalLv -.) tile absorhanice value ot ouhgaseous prod

  12. De vluchtige olie van mentha piperita L. gedurende de ontwikkeling van de plant

    NARCIS (Netherlands)

    Lemli, Joseph Albert Julia Melchior


    After a short description of the plant, the culture and the chemical composition of peppermint oil, a method is suggested for the determtnation of the total number of glandular hairs on the leaf. A new colorimetric determination of menthone and menthol, usins 2,4 - dinitr ophenylhy & azine is elabor

  13. Copper-catalyzed oxidative alkynylation of diaryl imines with terminal alkynes: a facile synthesis of ynimines. (United States)

    Laouiti, Anouar; Rammah, Mohamed M; Rammah, Mohamed B; Marrot, Jérome; Couty, François; Evano, Gwilherm


    An efficient copper-mediated method for the oxidative alkynylation of diaryl imines with terminal alkynes is reported. This reaction provides the first catalytic and general synthesis of ynimines and allows for an easy preparation of these useful building blocks. An improved copper-catalyzed oxidative dimerization of imines to azines and the synthesis of dienes and azadienes from ynimines are also described.

  14. Potassium (1-methoxycarbonyl-2-methylprop-2-en-2-ylideneazinate

    Directory of Open Access Journals (Sweden)

    Cédric Reuter


    Full Text Available In the title compound, K+·C6H8NO4−, the K+ cations have a coordination number of seven and are surrounded by four bidentate azinate anions. The methylene groups of the anions are always directed towards the coordinated potassium cations. The N—C—C—C torsion angle is 101.2 (2°. The orthogonal non-conjugated nature of the salt confirms the supposed geometry and reactivity of this compound.

  15. Drug: D00799 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D00799 Drug Trifluoperazine hydrochloride (JAN/USP); Stelazine (TN) C21H24F3N3S. 2H...azine structure N05AB06 Trifluoperazine D00799 Trifluoperazine hydrochloride (JAN/USP) USP drug classificati...on [BR:br08302] Antipsychotics 1st Generation/Typical Trifluoperazine D00799 Trifluopera...ptors Rhodopsin family Dopamine dopamine D2-receptor [HSA:1813] [KO:K04145] Trifluoperazine [ATC:N05AB06] D00799 Trifluopera

  16. In Situ Characterization of Polymer Matrices for Bio-electrode Applications

    Institute of Scientific and Technical Information of China (English)

    B.Y.Liaw; V.Svoboda; M.J.Cooney


    1 Results Electropolymerized azines are considered an important group of mediators for NAD+/ NADH-based biocatalytic applications[1].Characterizing these electroactive polymers in situ on electrode surface is vital to understand their behavior and properties.We recently studied the polymer deposition on electrodes using imaging ellipsometry (IE) as an in situ technique[2].The observation of surface morphology development can be conducted in cyclic voltammetric cycles in a nanometer scale.We then combine...


    Institute of Scientific and Technical Information of China (English)

    Zaza Gomurashvili; Allan S. Hay


    The N-N linkage in hydrazine is thermally stable when it is tetrasubstituted and there are electron-withdrawing groups attached to the nitrogen atoms. Our research on the synthesis of polymers containing N-N linkages is reviewed. The N,N-bisimide structure has been introduced in the backbone of polymers as well as a pendant group in poly(aryl ether)s to give very thermoxidatively stable polymers with very high glass transition temperatures. Most of the polymers form tough,flexible films. N-Aminotriazoles also form stable imides by reaction with anhydrides and these N-imidotriazole groups have been introduced as pendent groups on poly(aryl ether)s. The polymers are highly fluorescent materials. Three methods for the synthesis of polymers containing the tetrasubstituted-azine structure will be discussed: (1) oxidative coupling of bisimines;(2) condensation of bishydrazones with diketones; (3) poly(aryl ether azines) from the azine formed from 4-fluorobenzophenone by reaction with salts of bisphenols.

  18. C-H bond strengths and acidities in aromatic systems: effects of nitrogen incorporation in mono-, di-, and triazines. (United States)

    Wren, Scott W; Vogelhuber, Kristen M; Garver, John M; Kato, Shuji; Sheps, Leonid; Bierbaum, Veronica M; Lineberger, W Carl


    The negative ion chemistry of five azine molecules has been investigated using the combined experimental techniques of negative ion photoelectron spectroscopy to obtain electron affinities (EA) and tandem flowing afterglow-selected ion tube (FA-SIFT) mass spectrometry to obtain deprotonation enthalpies (Δ(acid)H(298)). The measured Δ(acid)H(298) for the most acidic site of each azine species is combined with the EA of the corresponding radical in a thermochemical cycle to determine the corresponding C-H bond dissociation energy (BDE). The site-specific C-H BDE values of pyridine, 1,2-diazine, 1,3-diazine, 1,4-diazine, and 1,3,5-triazine are 110.4 ± 2.0, 111.3 ± 0.7, 113.4 ± 0.7, 107.5 ± 0.4, and 107.8 ± 0.7 kcal mol(-1), respectively. The application of complementary experimental methods, along with quantum chemical calculations, to a series of nitrogen-substituted azines sheds light on the influence of nitrogen atom substitution on the strength of C-H bonds in six-membered rings.

  19. Application of 2-Trichloromethylbenzimidazole in Analytical Chemistry: A Highly Selective Chromogenic Reagent for Thin-Layer Chromatography and Some Other Analytical Uses

    Directory of Open Access Journals (Sweden)

    Leszek Konopski


    Full Text Available 2-Trichloromethylbenzimidazole (TCMB was used as a chromogenic reagent in organic or inorganic analysis, mainly in thin-layer chromatography (TLC. In reactions of TCMB with some heteroaromatic nitrogen containing compounds, such as azines, azoles and benzazoles, a formation of high colored products occurred. For azines, the chromogenic reaction was highly regioselective, since the both adjacent α-positions versus the nitrogen atom(s must not be substituted. A TLC method of detection was developed. Thirty azines, azoles, and benzazoles were detected at the detection limit 10 ng to 1 μg. This method was also applied for detection of heteroaromatic pesticides, and the attempts to construct active and passive dosimeters for nicotine were made. In a prechromatographic reaction of aromatic o-diamines with methyl trichloroacetimidate, TCMB or its derivatives were formed in situ. Followed by TLC and visualization in pyridine vapors, this procedure was applied for detection of o-phenylenediamine derivatives. The reaction product of TCMB and pyridine (LI Complex was identified and fully characterized. Two different reaction mechanisms: with electron deficient basic heteroaromatic compounds, like pyridine, and with more acidic compounds, for example, pyrrole, were discussed. In aqueous solutions, the LI Complex may be also used as a new indicator for complexometric, adsorption and acid-base titration of inorganic compounds.

  20. Conversion of 3-Carbethoxy-4-methyl Coumarin Derivatives into Several New Annelated Coumarin Derivatives

    Institute of Scientific and Technical Information of China (English)

    BAKEER; HadeerMohammed


    The reaction of ethyl esters of 4-methyl-2-oxo-2H-l-benzo(naphtho) pyran.3-carboxylic acids (1) with aromatic aldehy-des in the presence of piperidine yielded 4-styryl-3-carboxami-dopiperidyl connmrin derivatives 4. The reaction of hydrazlne hydrate with 1 gave acetophenone hydrozone derivatives 5 and acetophenone azine derivatives 6. The reaction of 1 with prima-ry amines afforded compomlds 7—9. And the treatment of la with Grignard reagents afforded 3-aroyl-4-methyl coumarin derivatives 10.

  1. Dicty_cDB: Contig-U10443-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 9_522( AE004969 |pid:none) Neisseria gonorrhoeae FA 1090, c... 132 3e-29 U66917_40( U66917 |pid:none) Pseudomonas sp. ADP atrazine...azine cataboli... 127 7e-28 DQ448807_1( DQ448807 |pid:none) Variovorax sp. MD1 plas... AP006627_2444( AP006627 |pid:none) Bacillus clausii KSM-K16 DNA, c... 127 5e-28 U66917_48( U66917 |pid:none) Pseudomonas sp. ADP atr

  2. Fluorescent property of 3-hydroxymethyl imidazo[1,2-a]pyridine and pyrimidine derivatives

    Directory of Open Access Journals (Sweden)

    Velázquez-Olvera Stephania


    Full Text Available Abstract Background Imidazo[1,2-a]pyridines and pyrimidines are important organic fluorophores which have been investigated as biomarkers and photochemical sensors. The effect on the luminescent property by substituents in the heterocycle and phenyl rings, have been studied as well. In this investigation, series of 3-hydroxymethyl imidazo[1,2-a]pyridines and pyrimidines were synthesized and evaluated in relation to fluorescence emission, based upon the hypothesis that the hydroxymethyl group may act as an enhancer of fluorescence intensity. Results Compounds of both series emitted light in organic solvents dilutions as well as in acidic and alkaline media. Quantitative fluorescence spectroscopy determined that both fused heterocycles fluoresced more intensely than the parent unsubstituted imidazo[1,2-a]azine fluorophore. In particular, 3-hydroxymethyl imidazo[1,2-a]pyridines fluoresced more intensely than 3-hydroxymethyl imidazo[1,2-a]pyrimidines, the latter emitting blue light at longer wavelengths, whereas the former emitted purple light. Conclusion It was concluded that in most cases the hydroxymethyl moiety did act as an enhancer of the fluorescence intensity, however, a comparison made with the fluorescence emitted by 2-aryl imidazo[1,2-a]azines revealed that in some cases the hydroxymethyl substituent decreased the fluorescence intensity.

  3. Pancreatic Kininogenase Ameliorates Renal Fibrosis in Streptozotocin Induced-Diabetic Nephropathy Rat

    Directory of Open Access Journals (Sweden)

    Dan Zhu


    Full Text Available Background/Aims: We aimed to evaluate whether pancreatic kininogenase (PKase can relieve renal fibrosis and investigate its mechanisms in diabetic nephropathy (DN rats Methods: We established streptozotocin (STZ induced-DN rats. After treatment with PKase for 4 weeks, urinary weight, urinary protein content and blood glucose concentration were detected, and then renal histopathological changes were examined using Hematoxylin and Eosin (H&E and Masson's thrchrome staining. In addition, the expressions of miR-433, transforming growth factor-β1 (TGF-β1 and antizyme inhibitor 1 (Azin1 were detected by qRT-PCR and/or western blotting. Results: PKase reduced urinary weight, urinary protein contents and blood glucose concentrations. PKase treated DN rats exhibited less renal fibrosis than untreated DN rats (P P P Conclusions: PKase might not only inhibit the development of DN by reducing urinary weight, urinary protein content and blood glucose concentration in DN rats, but also relieve renal fibrosis in DN rats through inhibiting the expression of TGF-β1, and miR-433 and Azin1 might involve in this process.

  4. Rh(II)-catalyzed Reactions of Diazoesters with Organozinc Reagents (United States)

    Panish, Robert; Selvaraj, Ramajeyam; Fox, Joseph M.


    Rh(II)-catalyzed reactions of diazoesters with organozinc reagents are described. Diorganozinc reagents participate in reactions with diazo compounds by two distinct, catalyst-dependent mechanisms. With bulky diisopropylethylacetate ligands, the reaction mechanism is proposed to involve initial formation of a Rh-carbene and subsequent carbozincation to give a zinc enolate. With Rh2(OAc)4, it is proposed that initial formation of an azine precedes 1,2-addition by an organozinc reagent. This straightforward route to the hydrazone products provides a useful method for preparing chiral quaternary α-aminoesters or pyrazoles via the Paul-Knorr condensation with 1,3-diketones. Crossover and deuterium labeling experiments provide evidence for the mechanisms proposed. PMID:26241081

  5. Polycyclic aromatic hydrocarbons connected with Schiff base linkers: Experimental and theoretical photophysical characterization and electrochemical properties (United States)

    Sęk, Danuta; Siwy, Mariola; Małecki, Jan Grzegorz; Kotowicz, Sonia; Golba, Sylwia; Nowak, Elżbieta M.; Sanetra, Jerzy; Schab-Balcerzak, Ewa


    A series of polyaromatic hydrocarbons with anthracene, phenanthrene and pyrene units connected with Schiff base junctions were synthesized via condensation of p-phenylenediamine and hydrazine with selected aldehydes. The effect of both hydrocarbon structures and presence of N-N- or phenyl- linked diimines on properties of the prepared azines and azomethines was analyzed. The obtained compounds were soluble in common organic solvents and melted in the range of 226-317 °C. Their photophysical and electrochemical properties were investigated by UV-vis, photoluminescence spectroscopies and cyclic voltammetry (CV), respectively. Moreover, a density functional theory (DFT) was applied for calculation of their electronic and geometric structures as well as absorption and emission spectra. Additionally, their electron acceptor activity was preliminary tested in photovoltaic experiment.


    Directory of Open Access Journals (Sweden)



    Full Text Available Oxazine derivatives are an important class of heterocycles, which has attracted much synthetic interest due to their wide range of biological activities. Oxazine is a heterocyclic compound can be formally derived from benzene, and its reduction products, by suitable substitution of carbon (and hydrogen atoms by nitrogen and oxygen. In the last few years oxazine derivatives have proved to be valuable synthetic intermediates and also possess important biological activities like sedative, analgesic, antipyretic, anticonvulsant, antitubercular, antitumour, antimalarial and antimicrobial. In these days, development of drug resistance is a major problem and to overcome this situation, it is necessary to synthesize new classes of compounds. The aim of the article is to review the generalization of the collected data about the synthesis of oxazine derivatives and their activities. We hope that this work will be a definite interest for researchers concerned with azines in generally and oxazines in particular.

  7. Pyrrole-Pyridine and Pyrrole-Naphthyridine Hosts for Anion Recognition

    Directory of Open Access Journals (Sweden)

    M. Angeles García


    Full Text Available The association constants of the complexes formed by two hosts containing pyrrole, amide and azine (pyridine and 1,8-naphthyridine groups and six guests, all monoanions (Cl−, CH3CO2−, NO3−, H2PO4−, BF4−, PF6−, have been determined using NMR titrations. The X-ray crystal structure of the host N2,N5-bis(6-methylpyridin-2-yl-3,4-diphenyl-1H-pyrrole- 2,5-dicarboxamide (1 has been solved (P21/c monoclinic space group. B3LYP/6-31G(d,p and calculations were carried out in an attempt to rationalize the trends observed in the experimental association constants.

  8. Library of UV-Vis-NIR reflectance spectra of modern organic dyes from historic pattern-card coloured papers. (United States)

    Montagner, Cristina; Bacci, Mauro; Bracci, Susanna; Freeman, Rachel; Picollo, Marcello


    An accurate characterisation of the organic dyes used in artworks, especially those made of paper, is an important factor in designing safe conservation treatments. In the case of synthetic organic dyes used in modern works of art, for example, one frequently encountered difficulty is that some of these dyes are not still commercially available. Recognizing this problem, the authors of this paper present the results of an analysis of UV-Vis-NIR fibre optic reflectance spectra of 82 samples of dyed paper prepared with 41 dyes. The samples come from a historic book, The Dyeing of Paper in the Pulp, which was published by Interessen-Gemeinschaft (I.G.) Farbenindustrie in 1925. The dyes used in the paper pulp belong to the azo compounds, acridine, anthraquinone, azine, diphenylmethane, indigoid, methine, nitro, quinoline, thiazine, triphenylmethane, sulphur and xanthene classes.

  9. Organoamido- and aryloxo-lanthanoids, 19. synthesis and structures of cisoid and transoid bis(1,2-dimethoxyethane)bis({eta}{sup 2}-pyrazolato)lanthanoid(II) complexes

    Energy Technology Data Exchange (ETDEWEB)

    Deacon, G.B.; Delbridge, E.E. [Monash Univ., Clayton (Australia). Dept. of Chemistry; Skelton, B.W.; White, A.H. [Western Australia Univ., Nedlands, WA (Australia). Dept. of Chemistry


    The complexes [Yb(bind){sub 2}(DME){sub 2}], [Yb(MePhpz){sub 2}(DME){sub 2}], [Yb(azin){sub 2}(DME{sub 2})], and [Eu(Ph{sub 2}pz){sub 2}(DMe){sub 2}] (bindH=4.5-dihydro-2H-benz[g]indazole; MePhpzH=3-methyl-5-phenylpyrazole; azinH=7-azaindole; Ph{sub 2}pzH=3,5-dephenylpyrazole; DME=1,2-dimethoxyethane) have been prepared by redox transmetallation between ytterbium or europium metal and the corresponding thallium(I) pyrazolate in tetrahydrofuran (THF) in the presence of mercury metal, followed by work up with DME. The thallium reagents were obtained by treatment of the appropriate pyrazole with thallium(I) ethoxide. Both [Yb(Ph{sub 2}pz){sub 2}(DME){sub 2}] and [Sm(Ph{sub 2}pz){sub 2}(DME){sub 2}] have been prepared by metathesis from LnI{sub 2}(THF){sub 2} and K(Ph{sub 2}pz) in THF, whilst the former has also been obtained by redox transmetallation from [Hg(Ph{sub 2}pz){sub 2}] and ytterbium metal and by reaction of 3,5-diphenylpyrazole with Yb(C{sub 6}F{sub 5}){sub 2}, and the latter from protolysis of [Sm{l_brace}N(SiMe{sub 3}){sub 2}{r_brace}{sub 2}(THF){sub 2}] with Ph{sub 2}pzH, followed in each case by crystallisation of the crude product from DME. Europium(II) 3,5-di-tert-butylpyrazolate was synthesised by a redox transmetallation/ligand exchange reaction between europium metal chunks, diphenylmercury(II), and 3,5-ditert-butylpyrazole (tBu{sub 2}pzH) in the presence of mercury metal in THF, and [Eu(tBu{sub 2}pz){sub 2}(DME){sub 2}] was isolated on crystallisation of the crude product from DME. The X-ray crystal structures of [Ln(L){sub 2}(DME){sub 2}] (Ln=Yb, L=bind or azin; Ln=Eu or Sm, L=Ph{sub 2}pz; Ln=Eu, L=tBu{sub 2}pz), each of a different crystallographic form, reveal eight-coordinate lanthanoid complexes with two {eta}{sup 2}-pyrazolate and two chelating DME ligands, but the structures differ in the relationship (cisoid or transoid) between the pyrazolate ligands. Thus cen-Ln-cen (cen=centre of the N-N bond) angles of 106.7 [Yb(bind){sub 2}(DME){sub 2

  10. Novel analytical reagent for the application of cloud-point preconcentration and flame atomic absorption spectrometric determination of nickel in natural water samples

    Energy Technology Data Exchange (ETDEWEB)

    Suvardhan, K. [Environmental Monitoring Laboratory, Department of Chemistry, S.V. University, AP (India); Rekha, D. [Environmental Monitoring Laboratory, Department of Chemistry, S.V. University, AP (India); Kumar, K. Suresh [Environmental Monitoring Laboratory, Department of Chemistry, S.V. University, AP (India); Prasad, P. Reddy [Environmental Monitoring Laboratory, Department of Chemistry, S.V. University, AP (India); Kumar, J. Dilip [Environmental Monitoring Laboratory, Department of Chemistry, S.V. University, AP (India); Jayaraj, B. [Department of Mathematics, S.V. University, Tirupati 517502, AP (India); Chiranjeevi, P. [Environmental Monitoring Laboratory, Department of Chemistry, S.V. University, AP (India)]. E-mail:


    Cloud-point extraction was applied as a preconcentration of nickel after formation of complex with newly synthesized N-quino[8,7-b]azin-5-yl-2,3,5,6,8,9,11,12octahydrobenzo[b][1,4,7,10,13] pentaoxacyclopentadecin-15-yl-methanimine, and later determined by flame atomic absorption spectrometry (FAAS) using octyl phenoxy polyethoxy ethanol (Triton X-114) as surfactant. Nickel was complexed with N-quino[8,7-b]azin-5-yl-2,3,5,6,8,9,11,12 octahydrobenzo[b][1,4,7,10,13]pentaoxacyclopentadecin-15-yl-methanimine in an aqueous phase and was kept for 15 min in a thermo-stated bath at 40 deg. C. Separation of the two phases was accomplished by centrifugation for 15 min at 4000 rpm. The chemical variables affecting the cloud-point extraction were evaluated, optimized and successfully applied to the nickel determination in various water samples. Under the optimized conditions, the preconcentration system of 100 ml sample permitted an enhancement factor of 50-fold. The detailed study of various interferences made the method more selective. The detection limits obtained under optimal condition was 0.042 ng ml{sup -1}. The extraction efficiency was investigated at different nickel concentrations (20-80 ng ml{sup -1}) and good recoveries (99.05-99.93%) were obtained using present method. The proposed method has been applied successfully for the determination of nickel in various water samples and compared with reported method in terms of Student's t-test and variance ratio f-test which indicate the significance of present method over reported and spectrophotometric methods at 95% confidence 0011lev.

  11. CYP-specific bioactivation of four organophosphorothioate pesticides by human liver microsomes. (United States)

    Buratti, Franca M; Volpe, Maria Teresa; Meneguz, Annarita; Vittozzi, Luciano; Testai, Emanuela


    The bioactivation of azinphos-methyl (AZIN), chlorpyrifos (CPF), diazinon (DIA), and parathion (PAR), four widely used organophosphorothioate (OPT) pesticides has been investigated in human liver microsomes (HLM). In addition, the role of human cytochrome P450 (CYPs) in OPT desulfuration at pesticide levels representative of human exposure have been defined by means of correlation and immunoinhibition studies. CYP-mediated oxon formation from the four OPTs is efficiently catalyzed by HLM, although showing a high variability (>40-fold) among samples. Two distinct phases were involved in the desulfuration of AZIN, DIA, and PAR, characterized by different affinity constants (K(mapp1) = 0.13-9 microM and K(mapp2) = 5- 269 microM). Within the range of CPF concentrations tested, only the high-affinity component was evidenced (K(mapp1) = 0.27-0.94 microM). Oxon formation in phenotyped individual HLM showed a significant correlation with CYP1A2-, 3A4-, and 2B6-related activities, at different levels depending on the OPT concentration. Anti-human CYP1A2, 2B6, and 3A4 antibodies significantly inhibited oxon formation, showing the same OPT concentration dependence. Our data indicated that CYP1A2 is mainly involved in OPT desulfuration at low pesticide concentrations, while the role of CYP3A4 is more significant to the low-affinity component of OPT bioactivation. The contribution of CYP2B6 to total hepatic oxon formation was relevant in a wide range of pesticide concentrations, being a very efficient catalyst of both the high- and low-affinity phase. These results suggest CYP1A2 and 2B6 as possible metabolic biomarkers of susceptibility to OPT toxic effect at the actual human exposure levels.

  12. Selective synthesis, structural studies and antitumor evaluation of some novel unsymmetrical 1-hetaryl-4-(2-chloroquinolin-3-ylazines

    Directory of Open Access Journals (Sweden)

    Samir Bondock


    Full Text Available A series of novel unsymmetrical 1-hetaryl-4-(2-chloroquinolin-3-yl azines 4–9 was selectively synthesized in a three-step procedure starting from acetanilide (1. The molecular structures of 4–9 were established by elemental analyses, spectral data, hybrid density functional theory (DFT and time-dependent density functional theory (TD-DFT calculations. Molecular conformation analysis for compounds 4–9, performed using DFT calculations utilizing the energy functional 3-Parameter (Exchange, Lee, Yang and Parr (B3LYP and the full-electron basis set Density Gauss double-zeta with polarization functions (DGDZVP, on the synthesized azines considering the torsion angles (θ1, θ2, θ3 revealed 8 plausible conformers for each compound. Electronic and thermodynamic properties including the dipole moment and the thermodynamic energy values of the Frontier occupied and virtual molecular orbitals, HOMO and LUMO, respectively, were calculated for the most stable conformer for each compound. Furthermore, TD-DFT calculations coupled with the polarizable conductor calculation model (PCM, performed on the most stable conformers in DMF to account for the solvent effect, revealed that the optical properties including λmax and oscillator strength performed on the most stable conformers were in excellent agreement with the experimental λmax and molar extinction coefficient, which clearly validate the most stable molecular conformers identified for compounds 4–9. Comparison of the biological results to the calculated electronic and thermodynamic properties showed that the cytotoxicity is dependent on the low-lying ELUMO because compound 8 has the lowest ELUMO value and exhibited the greatest antitumor activity.

  13. Laboratory Evaluation of the Toxicity of Systemic Insecticides to Emerald Ash Borer Larvae. (United States)

    Poland, Therese M; Ciaramitaro, Tina M; McCullough, Deborah G


    Emerald ash borer (Agrilus planipennis Fairmaire) (Coleoptera: Buprestidae), an invasive phloem-feeding insect native to Asia, threatens at least 16 North American ash (Fraxinus) species and has killed hundreds of millions of ash trees in landscapes and forests. We conducted laboratory bioassays to assess the relative efficacy of systemic insecticides to control emerald ash borer larvae in winter 2009 and 2010. Second- and third-instar larvae were reared on artificial diet treated with varying doses of emamectin benzoate (TREE-äge, Arborjet, Inc., Woburn, MA), imidacloprid (Imicide, J. J Mauget Co., Arcadia, CA), dinotefuran (Safari, Valent Professional Products, Walnut Creek, CA), and azadirachtin (TreeAzin, BioForest Technologies, Inc., Sault Ste. Marie, Ontario, and Azasol, Arborjet, Inc., Woburn, MA). All of the insecticides were toxic to emerald ash borer larvae, but lethal concentrations needed to kill 50% of the larvae (LC50), standardized by larval weight, varied with insecticide and time. On the earliest date with a significant fit of the probit model, LC50 values were 0.024 ppm/g at day 29 for TREE-äge, 0.015 ppm/g at day 63 for Imicide, 0.030 ppm/g at day 46 for Safari, 0.025 ppm/g at day 24 for TreeAzin, and 0.027 ppm/g at day 27 for Azasol. The median lethal time to kill 50% (LT50) of the tested larvae also varied with insecticide product and dose, and was longer for Imicide and Safari than for TREE-äge or the azadirachtin products. Insecticide efficacy in the field will depend on adult and larval mortality as well as leaf and phloem insecticide residues.

  14. Proton transfer assisted charge transfer phenomena in photochromic Schiff bases and effect of -NEt2 groups to the anil Schiff bases. (United States)

    Jana, Sankar; Dalapati, Sasanka; Guchhait, Nikhil


    Photochromic Schiff bases 5-diethylamino-2-[(4-diethylamino-benzylidene)-hydrazonomethyl]-phenol (DDBHP) and N,N'-bis(4-N,N-diethylaminosalisalidene) hydrazine (DEASH) with both the proton and charge transfer moieties have been synthesized, and their photophysical properties such as excited state intramolecular charge transfer (ICT) and proton transfer (ESIPT) processes have been reported on the basis of steady-state and time-resolved spectral measurement in various solvents. The ground-state six-membered intramolecular hydrogen bonding network at the proton transfer site accelerates the ESIPT process for these compounds. Both the compounds show large Stokes-shifted emission bands for proton transfer and charge transfer processes. The hydrogen bonding solvents play a crucial role in these photophysical processes. Excited-state dipole moment of DDBHP and DEASH calculated by the solvatochromic method supports the polar character of the charge transfer excited state. Introduction of -NEt(2) groups to the reported salicylaldehyde azine (SAA) Schiff base results an increase in fluorescence lifetime from femtosecond to picosecond time scale for the proton transfer process.

  15. 对《中国药典》2010年版磺胺嘧啶锌软膏鉴别试验的探讨%The Discussion on Identification Test of Sulfadiazine Zinc Ointment in Chinese Pharmacopoe-ia (2010 Edition)

    Institute of Scientific and Technical Information of China (English)

    杨淑先; 李少杰


    Objective:To improve the identification test of sulfadiazine zinc ointment in Chinese Pharmacopoeia ( 2010 Edi-tion).Methods: Increasing the sample quantity in the process of potassium ferrocyanide precipitation reaction to filtrate en-richment precipitation and reducing the amount of hydrochloric acid to reduce the total volume of the final solution and to make for the precipitation and enrichment.Results: The improved method can be used to identify sulfadiazine zinc ointment with a high specificity and effectiveness.Conclusion: The improved test method can be listed in the identification test in the sulfadi-azine zinc ointment.%目的:改进《中国药典》2010年版磺胺嘧啶锌软膏鉴别试验方法。方法:增加参与亚铁氰化钾沉淀反应的样品量,可滤过富集沉淀以进行后续的鉴别试验;减少盐酸用量,从而减少最终溶液总体积,更利于沉淀析出和富集。结果:改进后的方法可以专属而有效地鉴别磺胺嘧啶锌软膏。结论:改进后的试验方法可以用于磺胺嘧啶锌软膏的鉴别试验。

  16. Synthesis and Application of an Aldazine-Based Fluorescence Chemosensor for the Sequential Detection of Cu2+ and Biological Thiols in Aqueous Solution and Living Cells

    Directory of Open Access Journals (Sweden)

    Hongmin Jia


    Full Text Available A fluorescence chemosensor, 2-hydroxy-1-naphthaldehyde azine (HNA was designed and synthesized for sequential detection of Cu2+ and biothiols. It was found that HNA can specifically bind to Cu2+ with 1:1 stoichiometry, accompanied with a dramatic fluorescence quenching and a remarkable bathochromic-shift of the absorbance peak in HEPES buffer. The generated HNA-Cu2+ ensemble displayed a “turn-on” fluorescent response specific for biothiols (Hcy, Cys and GSH based on the displacement approach, giving a remarkable recovery of fluorescence and UV-Vis spectra. The detection limits of HNA-Cu2+ to Hcy, Cys and GSH were estimated to be 1.5 μM, 1.0 μM and 0.8 μM, respectively, suggesting that HNA-Cu2+ is sensitive enough for the determination of thiols in biological systems. The biocompatibility of HNA towards A549 human lung carcinoma cell, was evaluated by an MTT assay. The capability of HNA-Cu2+ to detect biothiols in live A549 cells was then demonstrated by a microscopy fluorescence imaging assay.

  17. The synthesis and spectroscopic study of stable free radicals related to piperidine-n-oxyl, including a stable bi-radical; Syntheses et etudes spectroscopiques de radicaux libres piperidiniques et d'un biradical stable, du type nitroxyde

    Energy Technology Data Exchange (ETDEWEB)

    Briere, R. [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires, Laboratoire de chimie organique physique


    A new synthesis of di-tert-butyl nitroxide using the reaction between tert-butyl magnesium chloride and nitro-tert-butane is presented in the first section. Synthesis and investigation of stable free piperidine-N-oxyl radicals are described in the second section. All these nitroxides have been characterised by their I. R., U. V. and E. P. R. absorption spectra. The final section contains a description of the synthesis of a stable bi-radical of the nitroxide type by condensation of 2,2, 6, 6-tetramethyl-piperid-4-one-l-oxyl with hydrazine. (author) [French] La premiere partie expose une nouvelle methode de synthase du di-t-butyl nitroxyde, par action d'halogenures de t-butyl magnesium sur le nitro-t-butane (Rdt maximum 45 pour cert, purete 86 pour cent). Une etude de radicaux. libres stables pipericliniques est faite dans une seconde partie. Ces composes sont obtenus par oxydation de derives de la triacetonamine. Les caracteristiques spectroscopiques ultra-violette, infra-rouge, et paramagnetique electronique de ces radicaux sont donnees. La grande inertie chimique du groupement nitroxyde a permis la syn-these d'un biradical stable par formation d'azine d'une cetone radicalaire, ce qui fait 1'objet de la troisieme partie. (auteur)

  18. Chromophores from photolyzed ammonia reacting with acetylene: Application to Jupiter's Great Red Spot (United States)

    Carlson, R. W.; Baines, K. H.; Anderson, M. S.; Filacchione, G.; Simon, A. A.


    The high altitude of Jupiter's Great Red Spot (GRS) may enhance the upward flux of gaseous ammonia (NH3) into the high troposphere, where NH3 molecules can be photodissociated and initiate a chain of chemical reactions with downwelling acetylene molecules (C2H2). These reactions, experimentally studied earlier by (Ferris and Ishikawa [1987] Nature 326, 777-778) and (Ferris and Ishikawa [1988] J. Amer. Chem. Soc. 110, 4306-4312), produce chromophores that absorb in the visible and ultraviolet regions. In this work we photolyzed mixtures of NH3 and C2H2 using ultraviolet radiation with a wavelength of 214 nm and measured the spectral transmission of the deposited films in the visible region (400-740 nm). From these transmission data we estimated the imaginary indices of refraction. Assuming that ammonia grains at the top of the GRS clouds are coated with this material, we performed layered sphere and radiative transfer calculations to predict GRS reflection spectra. Comparison of those results with observed and previously unreported Cassini visible spectra and with true-color images of the GRS show that the unknown GRS chromophore is spectrally consistent with the coupled NH3-C2H2 photochemical products produced in our laboratory experiments. Using high-resolution mass spectrometry and infrared spectroscopy we infer that the chromophore-containing residue is composed of aliphatic azine, azo, and diazo compounds.

  19. Synthesis, spectroscopic structure identification, X-ray study and anticancer activities of new angularly fused quinobenzothiazines (United States)

    Pluta, K.; Szmielew, M.; Suwińska, K.; Latocha, M.


    Synthesis of 16 new tetracyclic angularly fused azaphenothiazines, 8-, 9- and 10-substituted quinobenzo-1,4-thiazines (benzo[a]-3-azaphenothiazines) was based on the reactions of dichlorodiquinolinyl disulfide and diquinodithiin with substituted anilines. Whereas the reactions with p-fluoroaniline and p-methylthioaniline led to only one product, the reaction with m-triflouromethylaniline led to isomeric compounds. The obtained 8-10-substituted 12H-quinobenzothiazines were further transformed into 12-substituted derivatives through alkylation of the thiazine nitrogen atom. The structure analysis was based on 1D and 2D NMR (NOESY, COSY, HSQC and HMBC) spectra which enabled to distinguish the isomers and to exclude retro-Smiles rearrangement and the azine nitrogen atom alkylation pathways. This supposition was fully confirmed by X-ray analysis showing the quinobenzothiazine system to be folded and the substituent at the thiazine nitrogen atom in an equatorial position. Some compounds exhibited anticancer activity against MCF-7, MDA-MB-231 and SNB-19 cell lines similar to a reference drug cisplatin. The structure-activity relationship of the compounds were discussed.

  20. Molecular structure, spectroscopic (FT-IR, FT-Raman, NMR, UV) studies and first-order molecular hyperpolarizabilities of 1,2-bis(3-methoxy-4-hydroxybenzylidene)hydrazine by density functional method (United States)

    Subramanian, N.; Sundaraganesan, N.; Jayabharathi, J.


    Quantum chemical calculations of energies, geometrical structure and vibrational wavenumbers of 1,2-bis(3-methoxy-4-hydroxybenzylidene)hydrazine [vanillin azine (VA)] were carried out by using density functional (DFT/B3LYP) method with 6-31G(d) as basis set. The optimized geometrical parameters obtained by DFT calculations are in good agreement with single crystal XRD data. The vibrational spectral data obtained from solid phase FT-IR and FT-Raman spectra are assigned based on the results of the theoretical calculations. The observed spectra are found to be in good agreement with calculated values. The electric dipole moment ( μ) and the first hyperpolarizability ( β) values of the investigated molecule have been computed using ab initio quantum mechanical calculations. The calculation results also show that the VA molecule might have microscopic nonlinear optical (NLO) behavior with non-zero values. A detailed interpretation of the infrared and Raman spectra of VA was also reported. The energy and oscillator strength calculated by time-dependent density functional theory (TD-DFT) results complements with the experimental findings. The calculated HOMO and LUMO energies show that charge transfer occurs within the molecule. The theoretical NMR chemical shifts complement with experimentally measured ones.

  1. Chemical constituents and antioxidant activities of waste liquid extract from Apostichopus japonicus Selenka processing

    Institute of Scientific and Technical Information of China (English)

    LI Chaofeng; LI Xiancui; LI Hong; GUO Shuju; ZHU Xiaobin


    Apostichopus japonicus Selenka is an ideal tonic food that is used traditionally in many Asian countries,and it contains many bioactive substances,such as antioxidant,antimicrobial,and anticancer materials.To convert waste liquid generated during production into a useful resource,extract from waste liquid was isolated by column chromatography and studied by the pyrogallol autoxidation and 1,10-phenanthroline-Fe2+ oxidation methods.Results show that the extract scavenged about 91% of the superoxide anion radical at a concentration of 1.4 mg/mL and 24% of the hydroxyl radical at 3.3 mg/mL.Four compounds were isolated and identified from the extract:2,4-dihydroxy-5-methyl-1,3-azine; 2,4-dihydroxy-1,3-diazine; 3-O-[β-D-quinovopranosyl-(1→2)-4-O-sodium sulfate-β-D-xylopranosyl]-holosta-9(11)-ene-3β,12t,17α-triol; and 24-ethyl-5t-cholesta-7-ene-3β-O-β-D-xylopyranoside.All of these compounds are known in A.japonicus,and were found in the waste liquid for the first time.

  2. The Dysregulation of Polyamine Metabolism in Colorectal Cancer Is Associated with Overexpression of c-Myc and C/EBPβ rather than Enterotoxigenic Bacteroides fragilis Infection

    Directory of Open Access Journals (Sweden)

    Anastasiya V. Snezhkina


    Full Text Available Colorectal cancer is one of the most common cancers in the world. It is well known that the chronic inflammation can promote the progression of colorectal cancer (CRC. Recently, a number of studies revealed a potential association between colorectal inflammation, cancer progression, and infection caused by enterotoxigenic Bacteroides fragilis (ETBF. Bacterial enterotoxin activates spermine oxidase (SMO, which produces spermidine and H2O2 as byproducts of polyamine catabolism, which, in turn, enhances inflammation and tissue injury. Using qPCR analysis, we estimated the expression of SMOX gene and ETBF colonization in CRC patients. We found no statistically significant associations between them. Then we selected genes involved in polyamine metabolism, metabolic reprogramming, and inflammation regulation and estimated their expression in CRC. We observed overexpression of SMOX, ODC1, SRM, SMS, MTAP, c-Myc, C/EBPβ (CREBP, and other genes. We found that two mediators of metabolic reprogramming, inflammation, and cell proliferation c-Myc and C/EBPβ may serve as regulators of polyamine metabolism genes (SMOX, AZIN1, MTAP, SRM, ODC1, AMD1, and AGMAT as they are overexpressed in tumors, have binding site according to ENCODE ChIP-Seq data, and demonstrate strong coexpression with their targets. Thus, increased polyamine metabolism in CRC could be driven by c-Myc and C/EBPβ rather than ETBF infection.

  3. Chromophores from photolyzed ammonia reacting with acetylene: Application to Jupiters Great Red Spot (United States)

    Carlson, Robert W.; Baines, Kevin H.; Anderson, M. S.; Filacchione, G.; Simon, A. A.


    The high altitude of Jupiter's Great Red Spot (GRS) may enhance the upward flux of gaseous ammonia (NH3 ) into the high troposphere, where NH3 molecules can be photodissociated and initiate a chain of chemical reactions with downwelling acetylene molecules (C2H2 ). These reactions, experimentally studied earlier by (Ferris and Ishikawa [1987] Nature 326, 777-778) and (Ferris and Ishikawa [1988] J. Amer. Chem. Soc. 110, 4306-4312), produce chromophores that absorb in the visible and ultraviolet regions. In this work we photolyzed mixtures of NH3 and C2H2 using ultraviolet radiation with a wavelength of 214 nm and measured the spectral transmission of the deposited films in the visible region (400-740 nm). From these transmission data we estimated the imaginary indices of refraction. Assuming that ammonia grains at the top of the GRS clouds are coated with this material, we performed layered sphere and radiative transfer calculations to predict GRS reflection spectra. Comparison of those results with observed and previously unreported Cassini visible spectra and with true-color images of the GRS show that the unknown GRS chromophore is spectrally consistent with the coupled NH3-C2H2 photochemical products produced in our laboratory experiments. Using high-resolution mass spectrometry and infrared spectroscopy we infer that the chromophore-containing residue is composed of aliphatic azine, azo, and diazo compounds.

  4. Applications of electron spin resonance to some problems of radiation chemistry; Applications de la resonance paramagnetique electronique a quelques problemes de chimie sous rayonnements

    Energy Technology Data Exchange (ETDEWEB)

    Chachaty, C. [Commissariat a l' Energie Atomique Saclay (France). Centre d' Etudes Nucleaires


    The electron spin resonance (E.S.R.) spectra of gamma irradiated polar organic glasses, at 77 K, shows a single line centered at g {approx} 2, attributed to solvated electrons. The radicals produced on scavenging this species by electron acceptors, such as aromatic hydrocarbons, nitro-compounds and azines have been studied by E.S.R. In most cases, the radicals from these solutes, the spectra of which are observed after elimination by warming of the radicals from the matrices, are produced by protonation of the anions formed by scavenging of electrons at 77 K. Thus, in the case of glassy solutions of nitro-compounds, the radicals R NO{sub 2}H are formed. They are characterized by a{sub N} = 15 G (nitrobenzene) or a{sub N} = 28 G (nitro-alkane). These radicals are also generated by U.V, photolysis at room temperature of solutions of nitro-compounds in alcohols and are shown to be the precursors of nitroxide radicals R - N - R (with N - O) observed simultaneously. Gamma irradiation of solutions of pyridine and of the three diazines, in alcohol glasses at 77 K, produces the radical formed by hydrogen addition to these compounds. The value of the coupling constant of the additional proton (7-10 G) indicates that it is bound to a nitrogen in the sp{sup 2} hydridation state. One has shown, taking pyridine as an example, that the addition to a carbon gives a much greater value of the coupling constant, of the order of 50-60 G. (author) [French] Les spectres de resonance paramagnetique electronique (R.P.E.) obtenus apres irradiation gamma, a 77 K, de verres organiques polaires tels que les alcools, comportent une bande unique centree a g {approx} 2, attribuable aux electrons solvates. On etudie par R.P.E. les radicaux provenant de leur capture par des solutes ayant une affinite electronique, en particulier les hydrocarbures aromatiques, les composes nitres et les azines. En general, les radicaux provenant de ces solutes, dont on observe les spectres apres elimination

  5. Purification, characterization and synthetic application of a thermally stable laccase from Hexagonia tenuis MTCC-1119. (United States)

    Chaurasia, Pankaj Kumar; Bharati, Shashi Lata; Yadava, Sudha; Yadav, Rama Shanker Singh


    A thermally stable laccase was purified from the culture filtrate of Hexagonia tenuis MTCC-1119. The method involved concentration of the culture filtrate by ammonium sulphate precipitation and an anion-exchange chromatography on diethylaminoethyl (DEAE) cellulose. The sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and native polyacrylamide gel electrophoresis (native-PAGE) both gave single protein bands, indicating that the enzyme preparation was pure. The molecular mass of the enzyme determined from SDS-PAGE analysis was 100 kDa. The purification fold and percentage recovery of the enzyme activity were 12.75 and 30.12%, respectively. The pH and the temperature optima were 3.5 and 45 degrees C, respectively. The enzyme was most stable at pH 4.0 when exposed for 1 h. Using 2,6-dimethoxyphenol (DMP), 2,2 [azino-bis-(3-ethylbonzthiazoline-6-sulphonic acid) diammonium salt] (ABTS) and 3,5-dimethoxy-4-hydroxybenzaldehyde azine (syringaldazine) as the substrates, the K(m), k(cat) and k(cat)/K(m) values of the laccase were 80 μM, 2.54 s(-1), 3.17 x 10(4) M(-1)s(-1), 36 μM, 2.54 s(-1), 7.05 x 10(4) M(-1)s(-1) and 87 μM, 2.54 s(-1), 2.92 x 10(4) M(-1)s(-1), respectively. The purified laccase was finally used for the selective biotransformation of aromatic methyl group to aldehyde group in presence of diammonium salt of ABTS as the mediator and products were characterized by HPLC, IR and 1H NMR. The percentage yields of these transformed products were > 91%.

  6. Recent progress in biological activities of synthesized phenothiazines. (United States)

    Pluta, Krystian; Morak-Młodawska, Beata; Jeleń, Małgorzata


    This review summarizes recent medicinal chemistry investigations in vitro and in vivo in search for new phenothiazines of promising biological activities. New phenothiazine derivatives (over 50 main structures) contain dialkylaminoalkyl, cycloaminoalkyl and aminoalkyl substituents and their acyl and sulfonyl derivatives, and other substituents with varied the monocyclic (pyrazole, thiazole, oxadiazole, thiadiazole, tetrazole) and bicyclic (quinolizine, pyrazolopyrimidine, thiazolopyridine, azabicyclononane and spiro[chromanpyrimidine] heterocycles linked directly or via the alkyl chain with the thiazine nitrogen atom or with the benzene ring. The modifications of the tricyclic ring system with the bicyclic homoaromatic ring (naphthalene) and monocyclic and bicyclic azine rings (pyridine, pyrimidine, pyrazine and quinoline) led to compounds of significant biological activities. Recently obtained phenothiazines exhibit promising antibacterial, antifungal, anticancer, antiviral, anti-inflammatory, antimalarial, antifilarial, trypanocidal, anticonvulsant, analgesic, immunosuppressive and multidrug resistance reversal properties. These activities were the results of the actions of phenothiazines on biological systems via the interaction of the pharmacophoric substituent (in some cases of strict length), via the interaction of the multicyclic ring system (π-π interaction, intercalation in DNA) and via the lipophilic character allowing the penetration through the biological membranes. The activities were examined by using various biological systems such as cell lines, bacteria, viruses, parasites, laboratory mice, rats and rabbits, and monolayer and bilayer membranes. Some mechanisms of the actions are discussed. This review shows current tendency in the phenothiazine synthesis (without synthetic routes) and reveals the phenothiazine core to be very potent pharmacophoric moiety which can be a rich source of new compounds having desirable biological activities.

  7. Purification and characterization of laccase secreted by Phellinus linteus MTCC-1175 and its role in the selective oxidation of aromatic methyl group. (United States)

    Chaurasia, P K; Yadav, A; Yadav, S S; Yadava, S


    A laccase from the culture filtrate of Phellinus linteus MTCC-1175 has been purified to homogeneity. The method involved concentration of the culture filtrate by ammonium sulphate precipitation and an anion exchange chro- matography on DEAE-cellulose. The SDS-PAGE and native-PAGE gave single protein band indicating that the enzyme preparation was pure. The molecular mass of the enzyme determined from SDS-PAGE analysis was 70 kDa. Using 2,6-dimethoxyphenol, 2,2'[azino-bis-(3-ethylbonzthiazoline-6-sulphonic acid) diammonium salt] (ABTS) and 4-hydroxy-3,5-dimethoxybenzaldehyde azine as the substrates, the Kin, kcat and kt/Km values of the laccase were found to be 160 microM, 6.85 s(-1), 4.28 x 10(4) M(-1) s(-1), 42 microM, 6.85 s(-1), 16.3 x 10(4) M(-1) s(-1) and 92 microM, 6.85 s(-1), 7.44 x 10(4) M(-1) s(-1), respectively. The pH and the temperature optima of the P. linteus MTCC-1175 laccase were 5.0 and 45 degrees C, respectively. The activation energy for thermal denaturation of the enzyme was 38.20 kJ/mole/K. The enzyme was the most stable at pH 5.0 after 1 h reaction. In the presence ofABTS as the mediator, the enzyme transformed toluene, 3-nitrotoluene and 4-chlorotoluene to benzaldehyde, 3-nitroben-zaldehyde and 4-chlorobenzaldehyde, respectively.

  8. Study on The Features of The New Weekly%《新周刊》编辑特色探析

    Institute of Scientific and Technical Information of China (English)



    《新周刊》创刊于1996年8月18日,是由广东省新闻出版局和三九集团合办的一份以时事生活综合为内容的双周刊。以"中国最新锐的时事生活周刊"为宗旨,每期以敏锐的观察、独特的视角、新锐的选题、精美的图片、客观的语言、精彩的栏目为读者奉献一道道时事生活的文化大餐,现已成为时事生活类杂志的一枝独秀。主要从《新周刊》的选题叙述其编辑特色,同时从语言、栏目、封面、图片等方面强化叙述其编辑特色,并从中获得办刊启示。%The New Weekly is supervised by the News and Press Ministry of Guangdong and Sanjiu Company, aimed at current events and life. The first issue published in August 18th in 1996,double weekly publication. The New Weekly is for the purpose of the Chinese most radical new events life mag- azine. The New Weekly has special features as: keen observations, unique perspectives, sharp topics, fine pictures, objective features from its topic, enlightenment. language, brilliant columns for its readers. The author mainly described editorial as well as from language, programs, cover, pictures and so on, and to obtain the

  9. Purification and characterization of laccase from Coriolopsis floccosa MTCC-1177 and its use in the selective oxidation of aromatic methyl group to aldehyde without mediators

    Indian Academy of Sciences (India)

    P K Chaurasia; A Yadav; R S S Yadav; S Yadava


    A laccase from the culture filtrate of white rot fungus Coriolopsis floccosa MTCC-1177 has been purified to homogeneity. The method involved concentration of the culture filtrate by ammonium sulphate precipitation and an anion exchange chromatography on diethylaminoethyl (DEAE) cellulose. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) as well as native polyacrylamide gel electrophoresis (native-PAGE) produced single protein bands indicating that the enzyme preparation was pure. Molecular mass of the enzyme determined from SDS-PAGE analysis was 64 kDa. Using 2,6-dimethoxyphenol (DMP), 2,2'-[Azino-bis-(3-ethylbonzthiazoline-6-sulphonic acid (ABTS) diammonium salt and 4-hydroxy-3,5-dimethoxy benzaldehyde azine (syringaldazine) as the substrates, the m, cat and cat/m values of the laccase were found to be 112.5 M, 5.16 s-1, 4.60 × 104M-1 s-1, 58 M, 5.16 s-1, 8.90 × 104M-1 s-1 and 100 M, 5.16 s-1, 5.16 × 104M-1 s-1, respectively. The pH and temperature optima were 5.0°C and 40°C, respectively. Activation energy for thermal denaturation of the enzyme was 36.6 kJ/mol/K. The enzyme was most stable at pH4.0 when exposed for 1 h. The purified laccase has yellow colour and does not show absorption band around 610 nm found in blue laccases. The enzyme transforms toluene, 3-nitrotoluene and 4-chlorotoluene to benzaldehyde, 3-nitrobenzaldehyde and 4-chlorobenzaldehyde, respectively, in the absence of electron transfer mediators.

  10. Effects of putrescine on Atp8a1 gene expression in mouse NTH3T3 cells%腐胺对小鼠成纤维细胞中Atp8a1基因表达的影响

    Institute of Scientific and Technical Information of China (English)

    万涛; 李朝幸; 田园园; 王李英; 秦栋栋; 李凯; 曲嘉琳; 汤华


    目的:研究腐胺对小鼠成纤维细胞中Atp8a1基因表达的影响,初步探讨其作用机制.方法:用基因芯片和Real-time PCR检测正常小鼠和Azin1(Antizyme inhibitor1)基因敲除小鼠肝脏组织中Atp8a1基因的在mRNA水平上的表达;构建Atp8a1基因启动子的虫荧光素酶报告质粒pGL3-Atp8a1-P;将重组质粒转染NTH3T3成纤维细胞中,分别在含有4 μg/ml腐胺和不含腐胺的条件下,用双荧光素酶检测系统检测虫荧光素酶活性.结果:在含有腐胺的培养条件下,转染Atp8a1基因启动子虫荧光素酶报告质粒的细胞虫荧光素酶的活性明显改变.结论:腐胺能够抑制Atp8a1基因启动子活性而降低其在NIH3T3成纤维细胞中的表达.%Objective: To investigate the effects of putrescine on the expression of Atp8a1 in NIH3T3 cells and reveal its regulatory mechanism. Methods: DNA microarray and Real-time PCR were performed to detect the Atp8a1 gene expression in normal mouse liver and Azini knockout mouse liver. Atp8al promoter luciferase reporter plasmid, pGL3-Atp8a1-P, was constructed. NTH3T3 cells were transiently transfected with pGL3-Atp8a1-P, and cultured in medium with and without putreseine respectively. Then the luciferase activity was detected. Results: The relative luciferase activity was changed in the cells which were transfected with pGL3-Atp8a1-P when cultured in medium added with putrescine. Conclusion: Putrescine could down-regulate the Atp8a1 gene expression in NIH3T3 cells by inhibiting its promoter's activity.

  11. 沦陷时期北京文坛的“大众化”与“色情文学”%The Popurlarization and Pornography in Peking Literature Circle during the Period of Being Captured

    Institute of Scientific and Technical Information of China (English)



    “大众化”在中国现代文学史上占有重要的地位,沦陷时期的北京文坛通过三份影响广泛的刊物《中国公论》《中国文艺》与《国民杂志》亦表达了对“大众化”问题的关注。1939年春创刊的《中国公论》开启了北京文坛“大众化”讨论的帷幕,紧随而来的《中国文艺》将“大众化”讨论引向深入,特别是1942年前后在《国民杂志》上掀起的“色情文学”风潮,展现出彼时彼地“大众化”与众不同、发人深省的历史面貌。沦陷时期北京文坛的“色情文学”风潮不仅是对中国现代文学史上“大众化”问题的一种拓展,并且可能也是中国现代文学自身某一类气质的表现和释放,具有独特而耐人寻味的历史意义与认识价值。%The popurlarization is very important in the Chinese modern literature ,and Peking writers in the Period of Being Captured also pay attention to the problem which is seen obviously in three influential mag-azines -Chinese Opinions ,Chinese L iterature and People’s M agazine .Chinese Opinions ,started in 1939 , began the discussion of popularization of Beijing literatures ,and the following Chinese Literature deepened this popularizaiton .Especially the Pornography Talk in People’s Magazine around 1942 exhibits the differ-ent and profound face of the popurlarization .The Pornography Talk of Peking writers in the Period of Be-ing Captured expands the popurlarization in the Chinese modern literature ,and expresses one of tempera-ment of the Chinese modern literature .Therefore ,they have a peculiar and complicated meaning .

  12. The molecular and electronic states of 1,2,4,5-tetrazine studied by VUV absorption, near-threshold electron energy-loss spectroscopy and ab initio multi-reference configuration interaction studies (United States)

    Palmer, Michael H.; McNab, Hamish; Reed, David; Pollacchi, Anne; Walker, Isobel C.; Guest, Martyn F.; Siggel, Michele R. F.


    larger than the other known azines. The decline in diamagnetic susceptibility anisotropy in tetrazine, relative to benzene and pyridine, is indicative of lower resonance energy.

  13. Diverse ceramics of lithium synthesized by the combustion method; Diversos ceramicos de litio sintetizados por el metodo de combustion

    Energy Technology Data Exchange (ETDEWEB)

    Cruz G, D. [ININ, 52750 La Marquesa, Estado de Mexico (Mexico)


    Lithium ceramics would be applied as tritium breeder materials in the future fusion nuclear reactors. The present study shows a modified combustion synthesis to produce lithium ceramics using urea (CO(NH{sub 2}){sub 2}) - oxides (TiO{sub 2}, ZrO{sub 2}, Al{sub 2}O{sub 3})- hydroxide (LiOH) mixtures, that differ from the traditional combustion synthesis which utilizes, metal nitrates and fuels (urea/hydrazide, oxalyl dihydrazide, malonic acid dihydrazide, glycine, tetra formal tris azine, etc) mixtures in stoichiometric molar ratios to produce lithium ceramics In the present work, the modified combustion synthesis was performed to produce Li{sub 4}SiO{sub 4}, Li{sub 2}SiO{sub 3}, {beta}-Li{sub 2}TiO{sub 3}, m- Li{sub 2}ZrO{sub 3}, and {gamma}-LiAIO{sub 2}. It was necessary to add LiOH excess to balance Li{sub 2}O sublimation. The advantages and disadvantages of the modified combustion synthesis to prepare {beta}-Li{sub 2}TiO{sub 3} and m-Li{sub 2}ZrO{sub 3} ceramics were also studied. During synthesis were used insoluble oxide compounds. Although thermodynamic properties have been studied extensively from first principles, only limited insight exists about the kinetic properties of decomposition of lithium ceramics. In several works, Li{sub 4}SiO{sub 4} and Li{sub 2}SiO{sub 3} had shown high tritium solubility at lower temperatures than other tritium breeding materials. Therefore, we examined the thermal stability of these lithium silicates. Finally, the effect of 12000 kGy of {gamma} rays irradiation was analyzed in the lithium ceramics produced. The XRD analyses of irradiated samples showed decomposition of Li{sub 2}SiO{sub 3} to Li{sub 2}Si{sub 2}O{sub 5} due to radiolysis processes. Li{sub 4}SiO{sub 4} was decomposed to Li{sub 2}SiO{sub 3}. {beta}Li{sub 2}TiO{sub 3} did not decompose under {gamma} irradiation but m-Li{sub 2}ZrO{sub 3} decomposed to ZrO{sub 2}. Finally, {gamma}-LiAIO{sub 2} was stable to {gamma} irradiation. In general, consolidation effects

  14. Coordination Chemistry and Structural Dynamics of a Long and Flexible Piperazine-Derived Ligand. (United States)

    Hawes, Chris S; Hamilton, Sophie E; Hicks, Jamie; Knowles, Gregory P; Chaffee, Alan L; Turner, David R; Batten, Stuart R


    A long and highly flexible internally functionalized dipyridyl ligand α,α'-p-xylylenebis(1-(4-pyridylmethylene)-piper-4-azine), L, has been employed in the synthesis of a series of coordination polymer materials with Co(II), Cd(II), and Ag(I) ions. In poly-[Cd(L)(TPA)] 1 and poly-[Co(L)(IPA)], 2, (TPA = terephthalate, IPA = isophthalate) the ligand adopts a similar linear conformation to that seen in the structure of the unbound molecule and provides a long (2.6 nm) metal-metal bridging distance. Due to the mismatch of edge lengths with that provided by the carboxylate coligands, geometric distortions from the regular dia and (4,4) network geometries for 1 and 2, respectively, are observed. In poly-[Ag2(CF3SO3)2(L)], 3, the ligand coordinates through both pyridine groups and two of the four piperazine nitrogen donors, forming a high-connectivity 2-dimensional network. The compound poly-[Ag2(L)](BF4)2·2MeCN, 4, a porous 3-dimensional cds network, undergoes a fascinating and rapid single-crystal-to-single-crystal rearrangement on exchange of the acetonitrile guests for water in ambient air, forming a nonporous hydrated network poly-[Ag2(L)](BF4)2·2H2O, 5, in which the well-ordered guest water molecules mediate the rearrangement of the tetrafluoroborate anions and the framework itself through hydrogen bonding. The dynamics of the system are examined in greater detail through the preparation of a kinetic product, the dioxane-solvated species poly-[Ag2(L)](BF4)2·2C4H8O2, 6, which undergoes a slow conversion to 5 over the course of approximately 16 h, a transition which can be monitored in real time. The reverse transformation can also be observed on immersing the hydrate 5 in dioxane. The structural features and physical properties of each of the materials can be rationalized based on the flexible and multifunctional nature of the ligand molecule, as well as the coordination behavior of the chosen metal ions.

  15. Lung Cancer Signature Biomarkers: tissue specific semantic similarity based clustering of Digital Differential Display (DDD data

    Directory of Open Access Journals (Sweden)

    Srivastava Mousami


    /SAG, AIB1 and AZIN1 are significantly down regulated. All down regulated genes in this panel were highly up regulated in most other types of cancers. These panels of proteins may represent signature biomarkers for lung cancer and will aid in lung cancer diagnosis and disease monitoring as well as in the prediction of responses to therapeutics.

  16. 2012-2025 Roadmap of I.R.Iran’s Disaster Health Management (United States)

    Ardalan, Ali; Rajaei, Mohammad Hossein; Masoumi, Gholamreza; Azin, Ali; Zonoobi, Vahid; Sarvar, Mohammad; Vaskoei Eshkevari, khorshid; Ahmadnezhad, Elham; Jafari, Gelareh


    with a list of strategies and activities to be considered in operational planning and actions. However, a dynamic process of evaluation and revision is required to ensure that Iran’s health system goals are met by 2025. Address for correspondence: Ali Ardalan, No. 78, Italia Ave, Department of Disaster and Emergency Health, National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran. Email: or Citation: Ardalan A, Rajaei MH, Masoumi G, Azin A, Zonoobi V, Sarvar M, Vaskoei Eshkevari K, Ahmadnezhad E, Jafari G. 2012-2025 Roadmap of I.R.Iran’s Disaster Health Management. PLoS Currents Disasters. 2012 Jul 16 PMID:22953239

  17. Hydrolysis mechanisms of trans platinum-based anticancer drug with heterocyclic amine ligands: A comparative DFT study%杂环反铂(Ⅱ)抗癌药物水解反应机理的DFT研究

    Institute of Scientific and Technical Information of China (English)

    李添; 周立新; 李娟


    In this work, the hydrolysis processes of the anticancer drugs trans-[PtCl2 (piperi-dine) ( Am)], Am = 2.-pico]ine( 1), 3-picoline(2) , 4-picoiine(3), trans-[PtCl; (piperidine) ( piper-azine)] (4), trans-[PtCl2 ( piperazme )2 ] (5 ) and trans-[ PtCl2 (iminoether )2 ] (6 ) have been studied using hybrid density functional theory (B3LYP) and iso-electnc focusing polarized con-tinuum model CIEF-PCM) solvation models. The hydrolysis reactions leading to the activated drug form a key step for the reaction with the target DNA. The stationary points on the poten-tial energy surfaces for the first and second hydrolysis steps, proceeding via a general SN2 pathway, were fully optimized and characterized. It is found that the processes of the reactions follow the established theory for ligand substitution in square planar complexes,that is, substi-tution reactions usually occur via Pt-Iigand exchange reactions with a trigonal-bipyramidsl transition stale structure; the geometries of the transition states (TS) agree with the previous related work and all of the reactions are endothermic; the barrier height of the second hydroly-sis is always higher than that of the first step for all the systems. In comparison with previous work on cisplatin, a faster rate of hydrolysis is determined for the reaction. We make a com-parison with previous work on the hydrolysis processes of analogous trans platinum-based anti- cancer drugs. The results provide detailed energy profiles for the mechanism of hydrolysis of 1, 2, 3. 4, 5 and 6, which may assist in understanding the reaction mechanism of the drug with the DNA target and in the design of novel Pt-based anticancer drugs with trans geometries.%用DFT-B3LYP方法和IEF-PCM溶剂化模型研究了反铂抗癌药物trans-[PtCl2(piperidine)(Am)](Am=2-picoline(1).3-picoline(2),4 picoline(3)),trans-[PtCl2(piperidine)(piperazine)](4),trans-[PtCl2(piperazine)2](5)and trans-[PtCl2(iminoether)2](6)的水解过程.水解反应是药物与DNA靶

  18. 常见铁肥品种及其使用效果综述%Various types of iron fertilizers and their efficiency: A review

    Institute of Scientific and Technical Information of China (English)

    刘自飞; 高丽丽; 王盛锋; 刘荣乐; 汪洪


    Iron (Fe) is an essential microelement for plant growth. Although Fe is the most abundant nutrient in the mineral solid phase of soils, its presence in soil solution is negligible. Fe deficiency is a major nutritional disorder in crops, especially on calcareous soils. The solution for Fe deficiency by agronomic practices or by breeding and obtaining genetically modified plants is not efficient enough, therefore, the application of Fe fertilizers still remains the efficient strategy to overcome Fe deficiency. Inorganic Fe fertilizers ( e. g. , FeS04 · 7H2O) and Fe-complex fertilizers ( e. g. , lignosulfonates and humates) are frequently-used in domestic market. EDTA-Fe chelate is low stable, and EDTA can become diketopiper-azine, being the persistent organic pollutant in the environment. Synthetic Fe chelates ( e. g. , EDDHA/Fe3+ , EDDHMA/ Fe3 + , EDDHSA/Fe3 + ) either applied to the soil or delivered to the foliage, are common used in abroad. Also, some new slow-releasing Fe fertilizers and biodegradable Fe chelates ( e. g. , IDHA/Fe3 + , EDDS/Fe3 + ) have appeared in the market. This review covers the research progress on varieties, properties and effectiveness of Fe fertilizers in the recent years.%铁是植物正常生长必需的微量营养元素之一.铁虽然在土壤中的丰度很高,但植物可以吸收利用的有效铁很少,导致植物极易缺铁,尤其是在干旱、半干旱的石灰性土壤上,缺铁现象较为严重.利用农艺和生物技术手段解决植物缺铁的效果尚不十分明显,施用铁肥仍是纠正植物缺铁常用的有效方法.国内常用铁肥品种主要有硫酸亚铁为主的无机铁肥和一些有机物与铁复合形成的铁肥(木质素磺酸铁、腐殖酸铁).乙二胺四乙酸(EDTA)铁肥稳定性相对较低,且EDTA会转化成二酮哌嗪,成为持久性有机污染物.国外推广使用乙二胺二邻羟苯基乙酸(EDDHA)、乙二胺二(2-羟基-4-甲酰-苯基)乙酸(EDDHMA)、乙二胺二(2-羟基-4

  19. 非叠氮类气体发生剂的研究进展%Progress in Research of Non-azide Gas Generating Composition

    Institute of Scientific and Technical Information of China (English)

    刘影; 冯长根; 杨利


    非叠氮类气体发生剂具有的含氮量高、燃速快、安定性良好、无毒、绿色环保等优点,使其成为气体发生剂领域的研究热点,并在汽车安全气囊、航天器、消防灭火等方面有着广泛的应用前景.本文对比叠氮类气体发生剂的性能,详细介绍了唑类、嗪类、胍类和偶氮类非叠氮气体发生剂的性质;全面系统地综述了上述各类非叠氮气体发生剂国内外合成、表征与应用的研究进展;重点分析了各类典型非叠氮气体发生剂的配方及性能参数,并探讨配方内不同组分对发生剂不同性能的影响,为进一步研发新型非叠氮类气体发生剂配方提供参考.经研究发现,同传统的叠氮类气体发生剂相比,虽然非叠氮类气体发生剂在产气量、燃速等性能方面有较大的提高,但因其存在价格昂贵等问题,目前仍然没有完全取代叠氮类气体发生剂.因此,在现有研究基础上,还需积极研发更加环保、无毒、高效、廉价和实用的非叠氮类气体发生剂.%The non-azide gas generating composition is a hot research topic due to its many features, such as high nitrogen content, fast burning rate, good stability, non-toxicity, and green environmental protection. It is widely applied in airbag, spacecraft, fire fighting and other fields. In this paper, compared with the traditional azide gas generating composition, the properties of azole, azine, guanidine and azoic gas generating compositions are analyzed in detail: the progresses in the synthesis, the characterization and the application are reviewed: the formulas and characteristic parameters are discussed. It is found that the non-azide gas generating composition has not completely replaced the traditional azide gas generating composition, despite its superior properties in many aspects, because of factors like the high price, the excessive heat of combustion, and the high temperature. Therefore it is necessary to

  20. Pollution Characteristics of Antibiotics and Antibiotic-Resistant Bacteria and Genes in Wangyanggou River, Shijiazhuang, China%石家庄汪洋沟地区抗生素、抗性细菌和抗性基因污染特征

    Institute of Scientific and Technical Information of China (English)

    徐艳; 张远; 郭昌胜; 王红梅; 王丁明; 王凯; 李晓晨; 徐建


    chlortetracycline)and six sulfonamides(sulfadiazine, sulfamer-azine, sulfadimidine, sulfachinoxaline, sulfamethoxazole and trimethoprim)ranged from 5.81×101 to 3.87×105 ng·L-1 and 1.02×101 to 5.37× 103 ng·L-1 in water, and from 4.28×101 to 1.63×105 ng·g-1 and 1.18×101 to 1.68×104 ng·g-1 in sediments, respectively. The number of tetra-cycline and sulfonamide resistant bacteria in water was 4.00×101 to 2.13×104 CFU·mL-1 and 6.67×101 to 7.34×105 CFU·mL-1, respectively, 3 to 4 orders of magnitude lower than that in sediments. The abundance of five tetracycline resistant genes(tetA, tetB, tetE, tetW and tetZ), two sulfonamide(sul1and sul2)resistant genes and two integron genes(int1, int2)detected was relatively high in all samples. The genes, te-tA and sul1, were dominant resistant genes with relative abundance greater than 1.58 ×10-2 in Wangyanggou River. Principal component analysis indicated that the abundance of antibiotic-resistant genes might be affected by different pollution sources and water quality charac-teristics. The phylogenetic analysis of tet(B)showed that water quality change resulted in diverse resistant strains. The current results indi-cate that pollution of antibiotics, antibiotic-resistant genes in Wangyanggou River is more severe than in other rivers.

  1. Renovated treating method for the high-concentrated saline sewage from preparing hydrazine hydrate by means of ketazine process%酮连氮法制肼废水处理研究

    Institute of Scientific and Technical Information of China (English)

    李柏春; 黄会; 张文林; 许沉娜


    In this paper, we would like to introduce a renovated method for treating the high-concentrated saline sewage from preparing hydrazine hydrate by means of ketazine process, that is to say, a combined method that can integrate the chemical precipitation method and the sodium hypochlorite oxidation process into one to treat the sewage from preparing hydrazine hydrate in processing ketazine. The reason is that, on the part of sewage treatment, it can help to shift the treating process from the traditional removal to the recovery. As is known, the traditional biochemical method has been limited in use because the sewage to be treated is at low concentration of Chemical Oxygen Demand (CODCr)and high-salinity. Neither the chemical precipitation method can satisfy the need, for there come two chemicals containing Mg2+ and PO34- ions to be put into the sewage to generate the magnesium ammonium phosphate ( MAP) precipitation. Therefore, we prefer to choose MAP chemical precipitation technique for the MAP decomposition residues to be heated under the alkali condition can be reused in a recycling manner as magnesium and phosphate chemicals. Besides, the MAP chemical precipitation method enjoys a lot of advantages, such as simple in technology, fast in reaction speed, free from temperature limit, and high in the heavy-metal-ions-removing efficiency. To improve the removing efficiency, we have installed a special device for producing chlorine gas and sodium hypochlorite, which can be used as an oxidant in preparing azine. Since we have sufficient sodium hypochlorite, which is safe and convenient in use, it would be possible for us to use sodium hypochlorite to remove the sewage. Therefore, we have chosen the chemical precipitation method and the sodium hypochlorite oxidation process to treat the sewage under question in our research. The experimental results demonstrate that the ammonia removal efficiency could be achieved to 96.8% , when pH is regulated to 9.0, and the compo

  2. 激越/迟滞型抑郁症首次发作患者的认知功能%A study of cognitive function in patients with first-episode agitated and retarded major depressive disorder

    Institute of Scientific and Technical Information of China (English)

    李泾; 吴天诚


    Objective: To study the characteristics of cognitive function and influence factors by the neu-ropsychological tests in patients with first-episode agitated and retarded major depressive disorder. Method: 77 first-episode depressive participants were grouped according to the scores of agition and retardation in Hamilton depression scale( HAMD). Three or more points of each item were separately recrudited agitated-group(n = 20) or retarded-group (n = 24), less than three points in both items were non-interactive group (n =33). All participants and normal controls(n =40) were tested by Wisconsin card sorting test( WCST) ,continuous performance test ( CPT) and Wechsler memory test ( WMS) , to record the indexes and correlate with dosage of an-tipsychotics,clinical symptoms. Results:(1)There were wide cognitive impairment in depression patients(P< 0.05 or P <0. 01 ) :(2)A11 indexes of WCST,CPT parameters( number of cancel,number of commission, mean reaction time) and WMS indexes (memory quotient,short-term memory,immediate memory) in agitated and retarded groups were significantly different compared with non-interactive ones( P < 0. 05 or P < 0.01): (3)There were obvious diversities between agitated and retarded groups including non-preservative errors of WCST, number of commission and mean reaction time of CPT, short-term memory of WMS(p <0.05 or P <0. 01) :(4)No significant correlation was found between most of parameters and clinical symptoms or the amounts of psycho-tropic drugs (imipramine-equivalent dose of anti-depressants,diazepam-equivalent dose of sedatives,chlorprom-azine-equivalent dose of antipsychotics). Conclusion: The cognitive dysfunction of agitated depression pa-lients were not the same as those of retarded ones entirely,which supplied a positive evidence for the heterology of major depressive disorder.%目的:探讨激越型和迟滞型抑郁症首次发作患者的认知功能特征及其影响因素. 方法:77例抑郁症首次发作患者根

  3. Fluid inclusion and sulfur stable isotope evidence for the origin of the Ahangran Pb-Ag deposit

    Directory of Open Access Journals (Sweden)

    Mohammad Maanijou


    Full Text Available Introduction The Ahangaran Pb-Ag deposit is located in the Hamedan province, west Iran, 25 km southeast of the city of Malayer . . The deposit lies in the strongly folded Sanandaj-Sirjan tectonic zone, in which the ore bodies occur as thin lenses and layers. The host rocks of the deposit are Early Cretaceous carbonates and sandstones that are unconformably underlain by Jurassic rocks. Ore minerals include galena, pyrite, chalcopyrite, pyrrhotite and supergene iron oxide minerals. Gangue minerals consist of barite, dolomite, chlorite, calcite and quartz. The mineralization occurs as open-space fillings, veins, veinlets, disseminations, and massive replacements. Alteration consists of silicification, sericitization, and dolomitization. In this study, we carried out studies of mineralogy, microthermometry of fluid inclusions and sulfur isotopes to determine the source of sulfur and the physico-chemical conditions of formation. Materials and methods Seventy samples of different host rocks, alteration, and mineralization were collected from surface outcrops and different tunnels. Twenty of the samples were prepared for mineralogical studies at Tarbiat Modarres University in Tehran and 25 for petrological studies at the University of Bu-Ali Sina. Fluid-inclusion studies were done on 5 samples of quartz and calcite at Pouya Zamin Azin Company in Tehran using a Linkam THM 600 model heating-freezing stage (with a range of -196 to 480ºC. The accuracy and precision of the homogenization measurements are about ±1°C. Salinity estimates were determined from the last melting temperatures of ice, utilizing the equations by Bodnar and Vityk (1994 and for CO2 fluids using equations by Chen (1972. Nine samples of sulfides and barite were crushed and separated by handpicking under binocular microscope and powdered with agate mortar and pestle. About one gram of each sample was sent to the Stable Isotope and ICP/MS Laboratory of Queen’s University, Canada for

  4. 贝伐单抗治疗卵巢癌疗效及不良反应的系统评价%Adverse events risk associated with bevacizumab for ovarian cancer:a systematic re-view

    Institute of Scientific and Technical Information of China (English)

    陈小芸; 张栋栋; 范维; 郭钰珍


    目的:系统评价贝伐单抗治疗卵巢癌患者的疗效及不良反应,探讨贝伐单抗在卵巢癌治疗中的应用价值。方法:全面检索Medline、EMbase、PubMed、Cochrane Li-brary、Web of Science、CNKI、CBM、VIP及万方等数据库,并查阅相关领域的杂志。检索时限截至2013年11月。由两名独立研究者根据纳入及排除标准以及Cochrane Handbook 5.0的质量评价标准筛选文献,评价纳入研究的文献质量,并提取有效数据采用Review Manager 5.2软件进行Meta分析。结果:经筛选,共纳入5篇研究,4218例患者。结果显示,联合贝伐单抗治疗组能明显地增加患者的中位无进展生存时间,但中位生存时间之间差异无统计学意义。 Meta结果显示,联合贝伐单抗治疗组的高血压[ OR=3.73,95%CI (2.04,6.82),P<0.0001]、蛋白尿[OR=2.96,95%CI(1.13,7.75),P=0.03]、出血症状[OR=4.57,95%CI(3.58,5.84),P<0.00001]及伤口愈合并发症[OR=1.59,95%CI (1.11,2.28),P=0.01]等不良反应发生率较高,差异显著;两组的肠穿孔、肠脓肿及瘘、充血性心力衰竭、中性粒细胞减少、动脉和静脉血栓形成及可逆性后部白质脑病综合征( RPLS)不良反应的发生率无统计学差异。结论:贝伐单抗联合标准化疗能增加不良反应发生的风险,受研究质量及数量的限制,上述结论需更多高质量随机对照试验进一步证实。%Objective:To assess the effectiveness and safety of bevacizumab for ovarian cancer and explore the value of its clinical use. Methods:The database such as Medline、EM-base、PubMed、Cochrane Library、Web of Science、CNKI、CBM、VIP and WanFang Data were searched for randomized controlled trial(RCTs)from inception to Nov. 2013. And relevant mag-azine were also selected. Two reviewers independently screened literature according to the inclu-sion and exclusion criteria as well as the methods recommended by the Cochrane Handbook 5 . 0,extracted efficient data and