WorldWideScience

Sample records for ayu plecoglossus altivelis

  1. Protection against Flavobacterium psychrophilum infection (cold water disease) in Ayu fish (Plecoglossus altivelis) by oral administration of humus extract.

    Science.gov (United States)

    NAKAGAWA, Jun; IWASAKI, Tadashi; KODAMA, Hiroshi

    2009-11-01

    Humic substances are formed during the decomposition of organic matter in humus, and are found in many natural environments in which organic materials and microorganisms have been present. In the present study, oral administration of humus extract to ayu fish (Plecoglossus altivelis) induced effective protection against experimental Flavobacterium psychrophilum infection (cold water disease). Mortality of fish and development of skin lesions, such as erosion and hemorrhages on the skin, gill cover or mouth, were significantly suppressed in fish treated with 10%, 5% or 1% humus extract adsorbed on dry pellets. Although F. psychrophilum was not re-isolated from gills and erosion lesions of the skin of dead fish, bacterial gyrB DNA could be amplified in these specimens from dead fish and surviving control fish using the polymerase chain reaction. The protective effect of the extract was not the results of direct killing of bacteria or antibiotic activity of the extract since no obvious reduction in the bacterial number was observed at 5 times to 5,000 times dilution of the humus extract having pH 5.45 to 7.40. These results clearly show that treating fish with humus extract is effective in preventing cold water disease.

  2. Characterization of cadmium-related gene UraD in Ayu (Plecoglossus altivelis)%香鱼镉胁迫相关基因UraD的特征和表达分析

    Institute of Scientific and Technical Information of China (English)

    陈强; 陆新江; 陈炯; 史雨红

    2013-01-01

    2-氧-4-羟基-4-羧基-5-脲基咪唑啉脱羧酶(UraD)是嘌呤代谢反应酶,目前鱼类UraD基因仅在斑马鱼等少数物种得到克隆分析.从香鱼克隆获得UraD的cDNA序列,全长为967 bp,编码一个由174 aa组成的分子量为19.6 kDa的蛋白,等电点为5.42.系统进化树分析显示香鱼UraD位于鱼类UraD簇中,与爬行类和哺乳类的亲缘关系较远,香鱼与斑马鱼UraD的蛋白序列相似性最高达70%.香鱼UraD表达经过反转录PCR(RT-PCR)检测发现在香鱼肝、心和肠中具有较高表达.香鱼肝组织中UraD表达量经过实时荧光定量PCR(qRT-PCR)检测,发现镉胁迫下显著减少,香鱼UraD表达在盐度胁迫和鳗利斯顿氏菌感染下表达量保持不变.上述结果揭示香鱼UraD基因的基本特征,说明香鱼UraD基因表达与镉胁迫密切相关.%Ayu ( Plecoglossus altivelis ) is an osmerid-like fish living in streams and coastal waters of regions in East Asia countries, especially China, Japan, and Korea. As the life cycle of ayu spans river, brackish and seawaters, this would be a suitable fish model for investigating the responses of aquatic organisms to heavy metals like cadmium (Cd). 2-Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (UraD) , an enzyme involved in urate degradation, plays an important role in nucleotide metabolism, which is sensitive in response to heavy metals. In this study, the physiological response to heavy metals in fish was studied. The ayu UraD gene was obtained by degenerate primer and rapid amplification of cDNA ends ( RACE). The cDNA full-length of ayu UraD was 967 nucleotides contained a complete open reading frame ( ORF) encoding a 174-amino-acid peptide with a molecular weight of 19. 6 kDa and an isoe-lectric point of 5. 42. Sequence comparison and phylogenetic analysis showed that ayu UraD was most similar to zebrafish ( Derio re-rio) , and the relationships of the different UraDs coincided well with the evolutionary relationships of

  3. Biochemical genetic analysis of isozymes in Plecoglossus altivelis population in Fuxi%凫溪香鱼群体同工酶的生化遗传分析

    Institute of Scientific and Technical Information of China (English)

    黄福勇; 李明云

    2004-01-01

    30 ayu(Plecoglossus altivelis) samples were collected from the cultural net in Fuxi Town, Ninghai County, Zhejiang Province. Polyarylamide gel electrophoresis was used to detect the expression of isozymes in 8 organs or tissues: eye, liver, kidney, muscle, spleen, heart, gill and pectroral fin. ADH,CAT,POD,ACP,ALP,EST,LDH,MDH,ME,GcDH,SCD,GDH,SDH,SOD,ATP were analyzed and the biochemical genetic results showed that 15 isozymes were coded by 55 gene loci, 12 of which (ADH-1, ADH-4, CAT-2, POD-3,ALP-2, EST-3, EST-4, LDH-5, ME-4, GcDH-4, GDH, SDH-2) were found polymorphic. The population of ayu (Plecoglossus altivelis) in Fuxi town showed the higher genetic diversity when compared with other freshwater fishes, with 21.8% of the proportion of polymorphic loci and 0.0459 of the average heterozygosity per loci. And we think the expression of CAT, POD and SOD of ayu (Plecoglossus altivelis) can be used to evaluate the influence to body-protecting system of the fish when environmental factors were changed. The expression of isozymes in pectroral fin can be used as genetic marker in breeding of ayu (Plecoglossus altivelis)

  4. 香鱼抗凝血酶基因(AT)cDNA的克隆、序列分析及组织表达特征%Cloning, Sequence Analysis and mRNA Expression Pattern of Antithrombin Gene (A T) in Ayu(Plecoglossus altivelis)

    Institute of Scientific and Technical Information of China (English)

    李长红; 陈炯; 史雨红; 陆新江

    2011-01-01

    抗凝血酶(antithrombin,AT)主要抑制凝血酶(thrombin)及其它凝血因子的活性,并具有重要的抗炎活性.本实验从香鱼(Plecoglossus altivelis)肝组织cDNA文库中成功获得了香鱼AT基因的cDNA全序列(EMBL登录号二FN429980).测序结果表明,香鱼AT基因cDNA全长1 487个核苷酸,包含1个大的开放阅读框(ORF),推测编码1个由453个氨基酸组成的蛋白,N端23个氨基酸为信号肽序列.成熟AT具有与哺乳动物AT相似的特征结构,包括:羧基末端附近的丝氨酸蛋白酶活性中心、6个保守的Cys残基形成的3个二硫键结构和4个N-糖基化位点.氨基酸序列分析表明,香鱼AT与大西洋鲑(Salmo salar)AT氨基酸序列同源性最高,为81%.系统进化树分析表明,物种AT的进化关系与目前接受的物种分类关系基本一致,香鱼AT位于鱼类AT簇中,与大西洋鲑、红鳍东方豚(Takifugu rubripes)和斑马鱼(Danio rerio)AT进化相关性最高.AT mRNA在健康香鱼的肝组织中表达量最大,在脾、肾和脑中表达量较低.实时荧光定量PCR结果表明,在鳗利斯顿氏菌(Listonella anguillarum)侵染4和8h的香鱼肝组织中AT mRNA表达量显著下调(P<0.05),但随着病程发展,12~36 h,A T mRNA表达水平显著上调(P<0.05),揭示AT是一个病程相关蛋白,并参与香鱼细菌感染导致的急性期反应.%Antithrombin (AT) is the major inhibitor of thrombin and other serine proteinases comprising the coagulation cascade enzymes. Furthermore, it has anti-inflammatory properties. The nucleotide sequence of full-length cDNA clone of ayu (Plecoglossus altivelis)AT gene (EMBL accession: No. FN429980) was obtained from the constructed liver cDNA of ayu. The whole cDNA length of ayu AT gene was 1 487 bp, consisting of a 1 362 bp open reading frame (ORF). The predicted mature ayu AT consisted of 453 amino acids preceded by a signal peptide of 23 residues. The structure of mature AT in ayu was similar with those in the mammalian AT

  5. Molecular cloning, sequence analysis and expression analysis of ApoC-Ⅰgene from ayu (Plecoglossus altivelis) exposed to cadmium%香鱼 ApoC-Ⅰ的克隆、序列分析及其镉胁迫下的表达

    Institute of Scientific and Technical Information of China (English)

    陈亚萍; 丁斐斐; 李长红; 默立宾; 陈炯

    2016-01-01

    ApoC-Ⅰ( Apolipoprotein C-Ⅰ) is the smallest member of the apolipoprotein family , which plays important roles in lipid uptake, secretion and transport .Here, we obtained the full-length cDNA sequence of ayu ( Plecoglossus altivelis ) ApoC-I gene ( PaApoC-Ⅰ) through random sequencing of cDNA library .The cDNA sequence of PaApoC-Ⅰis 574 nucleotides , consisting of a 267 bp open reading frame that encodes a protein of 88 amino acids with a deduced molecular mass of 10.08 ku.The N-terminus of PaApoC-Ⅰcontains a predicted signal peptide of 20 amino acids .Multiple sequence alignment of complete amino acid sequences showed that PaApoC-Ⅰshared the highest amino acid sequence identity (67.0%) with that of rainbow trout ( Oncorhynchus mykiss) . Phylogenetic tree analysis revealed that ApoC-Ⅰfrom mammals, reptiles, amphibians and fish formed different clusters , and PaApoC-Ⅰfalled into the fish ApoC-Ⅰcluster and was most closely related to that of rainbow trout .Genome structure analysis showed that PaApoC-Ⅰcontained three exons and two introns .Quantitative real-time PCR ( qRT-PCR) analysis showed that PaApoC-ⅠmRNA had the highest expressional level in ayu liver .Upon cadmium (Cd) exposure, PaApoC-I transcripts were significantly up-regulated in ayu liver, spleen, kidney and intestine, and were significantly down-regulated in ayu gill.In summary, our present study demonstrated that a tight relationship between the PaApoC-Ⅰexpression and Cd stress , and it can be a potential biomarker for Cd exposure in aquatic or-ganisms.The results would provide the basic data for further studying the function of fish ApoC-Ⅰand its mechanism in fish response to heavy metal stress .%载脂蛋白C-Ⅰ( Apolipoprotein C , ApoC-Ⅰ)是载脂蛋白家族中分子质量最小的成员,在脂类摄入、转运和分泌中发挥重要作用。通过cDNA文库随机测序获得了香鱼( Plecoglossus altivelis) ApoC-Ⅰ( PaApo

  6. The establishment of a library screening method based on yeast two-hybrid system and its use to determine the potential interactions of liver proteins in ayu, Plecoglossus altivelis.

    Science.gov (United States)

    Shi, Y H; Chen, J; Li, C H; Yang, H Y; Lu, X J

    2011-01-01

    Knowledge of specific protein-protein interaction (PPI) is an important component in understanding biological processes and regulatory mechanisms. A library to library screening method (LLS) was established based on yeast two-hybrid (YTH) system in this research, and applied to study the PPIs in ayu liver. In total, 23 out of 55 interaction pairs were found positive through phenotypic identification, with a positive rate of 41.8%. Of the 11 unique PPIs, 9 interactions including FGB/FGG, CaM/Spna2, C9/Apo-AI-1, α₂M/Ft, RPL10/RPL5, C8α/C9, FGG/Apo-AI-1, LECT2/Tf, and Apo-AI-2/C9 were previously reported. The other two PPIs including FGG/CLR and Wap65/C3 are novel, and in vitro co-immunoprecipitation (co-IP) experiments further confirmed these interactions. FGG/CLR interaction might play a role in regulating the inflammatory response. The interaction between Wap65 and C3 hints that Wap65 might function through the complement activation pathways when microbial infection occurs.

  7. Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis.

    Science.gov (United States)

    Chen, Qiang; Lu, Xin-Jiang; Li, Ming-Yun; Chen, Jiong

    2016-03-18

    Colony-stimulating factor 1 receptor (CSF-1R) is an important regulator of monocytes/macrophages (MO/MΦ). Although several CSF-1R genes have been identified in teleosts, the precise role of CSF- 1R in ayu (Plecoglossus altivelis) remains unclear. In this study, we characterized the CSF-1R homologue from P. altivelis, and named it PaCSF-1R. Multiple sequence alignment and phylogenetic tree analysis showed that PaCSF-1R was most closely related to that of Japanese ricefish (Oryzias latipes). Tissue distribution and expression analysis showed that the PaCSF-1R transcript was mainly expressed in the head kidney-derived MO/MΦ, spleen, and head kidney, and its expression was significantly altered in various tissues upon Vibrio anguillarum infection. After PaCSF-1R neutralization for 48 h, the phagocytic activity of MO/MΦ was significantly decreased, suggesting that PaCSF-1R plays a role in regulating the phagocytic function of ayu MO/MΦ.

  8. Assessing carcinogenic risks associated with ingesting arsenic in farmed smeltfish (Ayu, Plecoglossus altirelis) in aseniasis-endemic area of Taiwan.

    Science.gov (United States)

    Lee, Jin-Jing; Jang, Cheng-Shin; Liang, Ching-Ping; Liu, Chen-Wuing

    2008-09-15

    This study spatially analyzed potential carcinogenic risks associated with ingesting arsenic (As) contents in aquacultural smeltfish (Plecoglossus altirelis) from the Lanyang Plain of northeastern Taiwan. Sequential indicator simulation (SIS) was adopted to reproduce As exposure distributions in groundwater based on their three-dimensional variability. A target cancer risk (TR) associated with ingesting As in aquacultural smeltfish was employed to evaluate the potential risk to human health. The probabilistic risk assessment determined by Monte Carlo simulation and SIS is used to propagate properly the uncertainty of parameters. Safe and hazardous aquacultural regions were mapped to elucidate the safety of groundwater use. The TRs determined from the risks at the 95th percentiles exceed one millionth, indicating that ingesting smeltfish that are farmed in the highly As-affected regions represents a potential cancer threat to human health. The 95th percentile of TRs is considered in formulating a strategy for the aquacultural use of groundwater in the preliminary stage.

  9. Morphology of the digestive tract and liver of Plecoglossus altivelis%香鱼消化道及肝脏的形态结构特征

    Institute of Scientific and Technical Information of China (English)

    卢明明; 李海燕; 竺俊全; 李明云

    2012-01-01

    The morphological characteristics of the digestive tract and liver in Plecoglossus altivelis were observed by paraffin section and photomicrography techniques. The digestive tract includes the orol-pharyngeal cavity, esophagus, stomach and intestine. The orol-pharyngeal cavity is big, long and narrow; in the front of its bottom wall there are a pair of mucous plait; on the edge of the two jaws there are broad flat pectinate jaw teeth; there are teeth on the palate bone and hyoid bone, but no teeth on the vomer. Tongue is formed by the projecting part of basihyoid covered by mucosa. Tongue mucosa epithelium is stratified squamous epithelium, and contains many goblet cells and taste buds. The esophagus, stomach and intestines all consist of mucous membrane layer, submucosa, muscularis and outer membrane. The mucosa of esophagus is stratified squamous epithelium with developed goblet cells. V-shaped stomach comprises cardia, body of stomach and pylorus. Stomach mucosa epithelium is simple columnar epithelium. There are gastric glands in the lamina propria of cardia and body of stomach. Intestine is short and consists of foregut, midgut and hindgut. The mucous epithelium of intestinal wall is simple columnar epithelium and the free surface of it contains a dense array of microvilli. Lots of goblet cells are distributed in the intestinal epithelium. Conjunctive sites of intestines and pylorus contain 350 — 400 pyloric caecums whose histological structure is the same as intestines. The liver is singular and coated by the serosa. The shape of hepatocyte is irregular and the boundaries of hepatic lobules are inconspicuous.%采用解剖及石蜡切片显微技术观察了香鱼消化道及肝脏的组织学结构.香鱼消化道由口咽腔、食道、胃及肠构成.口咽腔大且狭长,其底壁前部有一对粘膜褶,两颌边缘着生宽扁梳状齿,腭骨及舌骨具齿,犁骨无齿;舌由基舌骨突出部分覆盖粘膜构成,舌粘膜上皮为复层扁平上

  10. 饵料系列对香鱼(Plecoglossus altivelis Temminck et Schlegel)人工育苗成活率、畸形率和白化率的影响%Effect of Bait Series on the Rates of Survival, Abnormal and Albinism for Artificial Breeding of Ayu(Plecoglossus altivelis)

    Institute of Scientific and Technical Information of China (English)

    刘招坤

    2008-01-01

    通过同一饵料系列(轮虫、卤虫无节幼体、桡足类、颗粒配合饲料)中不同的轮虫投喂时间,桡足类、颗粒配合饲料的比例,比较研究香鱼人工育苗的成活率、畸形率、白化率等育苗效果,以完善香鱼全人工批量育苗工艺.试验表明,在香鱼人工育苗仔稚鱼培育早期延长轮虫的投喂时间到15日龄可提高育苗的成活率及苗种规格;增加桡足类投喂量、减少颗粒配合饲料的投喂或采用研制全价的颗粒配合饲料有助于降低苗种的白化率;苗种的畸形与卵质有直接的关系,但同时与饵料、水质条件等因素有关.

  11. Construction and Characterization of a cDNA Library in Hepatopancreas of Ayu Plecoglossus altivelis%香鱼肝胰腺cDNA文库的构建和鉴定

    Institute of Scientific and Technical Information of China (English)

    张亮; 史雨红; 陈炯; 陆新江; 李明云

    2011-01-01

    以香鱼正常肝胰腺为材料,构建cDNA文库.初始文库库容大于1.3×107 cfu/ml,扩增文库的滴度大于1.1×106cfu/ml,平均插入片段约为1.0 kb.从文库中随机挑选297个cDNA克隆测序,得到232个功能已知EST序列,功能包括代谢、蛋白质合成、细胞与机体防御、信号转导以及细胞结构与运动等5个方面.随机测序获得香鱼弹性蛋白酶(Elastase,ELA)编码序列,cDNA序列全长957个核苷酸,3'-末端具有polyA尾,编码一个由268个氨基酸组成28.7 ku大小蛋白.序列分析表明,香鱼ELA的N端15个氨基酸为信号肽,成熟肽具有丝氨酸蛋白酶家族的典型结构特征.香鱼ELA与斜带石斑鱼、大西洋鳕和金头鲷的ELA氨基酸序列同源性最高,达76%~77%.系统进化树分析表明,香鱼ELA与其他鱼类ELA紧密成簇.RT-PCR检测显示,香鱼ELA基因mRNA在香鱼肝胰腺、脾、肠组织中均大量表达.

  12. Ion microprobe Sr isotope analysis of carbonates with about 5 {mu}m spatial resolution: An example from an ayu otolith

    Energy Technology Data Exchange (ETDEWEB)

    Sano, Yuji [Center for Advanced Marine Research, Ocean Research Institute, University of Tokyo, Nakano-ku, Tokyo 164-8639 (Japan)], E-mail: ysano@ori.u-tokyo.ac.jp; Shirai, Kotaro [International Coastal Research Center, Ocean Research Institute, University of Tokyo, Otsuchi, Iwate 028-1102 (Japan); Takahata, Naoto; Amakawa, Hiroshi [Center for Advanced Marine Research, Ocean Research Institute, University of Tokyo, Nakano-ku, Tokyo 164-8639 (Japan); Otake, Tsuguo [International Coastal Research Center, Ocean Research Institute, University of Tokyo, Otsuchi, Iwate 028-1102 (Japan)

    2008-08-15

    A high lateral resolution secondary ion mass spectrometer (NanoSIMS NS50 ion microprobe) has been used to measure {sup 87}Sr/{sup 86}Sr ratios in natural CaCO{sub 3} samples. A {approx}2 nA O{sup -} primary beam was used to sputter a 5-7-{mu}m diameter crater on the sample surface and secondary positive ions were extracted for mass analysis using an accelerating voltage of 8 kV and a Mattauch-Herzog geometry. The multi-collector system was adjusted to detect {sup 43}Ca{sup +} (by a detector EM2), {sup 80}Ca{sub 2}{sup +} (EM3), {sup 86}Sr{sup +} (EM4), and {sup 87}Sr{sup +} (EM4b) ions at the same time. Then the magnetic field was scanned for the EM4 to detect {sup 85}Rb{sup +}, {sup 86}Sr{sup +} and {sup 87}Sr{sup +}, while the EM4b can measure {sup 86}Sr{sup +}, {sup 87}Sr{sup +} and {sup 88}Sr{sup +}, respectively. A mass resolution of 3600 at 10% peak height was attained with a flat peak top, while the sensitivity of Sr was about 10 cps/nA/ppm. An aragonite sample (coral skeleton standard; JCp-1) was used as a reference for Sr isotope ratio calibration. Repeated analyses of the JCp-1 show that the {sup 87}Sr/{sup 86}Sr ratio agrees well with the seawater signature within a precision of 0.3 per mille at 2{sigma}, after the series of corrections such as the Ca dimer, {sup 87}Rb, and a mass bias estimated by the {sup 88}Sr/{sup 86}Sr ratio. The method was applied to an otolith (ear-stone) from ayu (Plecoglossus altivelis altivelis) collected from the Yodo river, Japan. Although experimental errors are relatively large, up to 3 per mille at 2{sigma}, the ratios of the core region are higher than the seawater signature while more distal values agree well with seawater. The very outermost part again shows the relatively higher {sup 87}Sr/{sup 86}Sr ratios. The spatial variation of {sup 87}Sr/{sup 86}Sr ratios was consistent with amphidromous migration of the fish, namely, born in the lake and grown in the coastal sea and finally collected in a river.

  13. Importance of Ayu Pareeksha for the Management of Diseases

    Science.gov (United States)

    Agrawal, Monika; Dwibedy, BK

    2017-01-01

    The word Ayurveda comes from the Sanskrit root Ayu which means span of life. The purpose of examination of Ayu is to obtain knowledge regarding longevity, residual span of life in diseased person and rate of the mortality. The purpose of Dashavidha Pariksha is to obtain knowledge regarding Dosha Bala (Vyadhi Bala), Bala (Rogibala) and Ayu of the patient as described in Charak Samhita. Commonly Ayu and Vaya are regarded as synonyms but they are not similar terms. Hence, description of Vaya and Ayu is separately mentioned in Charaka as well as Sushruta Samhita. Description exclusively on Ayu Pareeksha described in Charaka Viman Sthana, Charaka Chikitsasthana, Charaka Indriya Sthana and Charaka Shareer Sthana. In Indriya Sthana, Ayu Pariksha indicates the residual life-span of the diseased person and where as in Jaatisutriya Adhyaya in Shareer Sthana mentioned the characteristic properties of the child who is going to survive for longer period of time. In Sushruta Samhita, Ayu is classified into three broad headings namely Dhirghayu, Madhyamayu and Alpayu and explained the characteristic properties of all the three.

  14. 潜孔の設置位置がアユの遡上特性に与える影響

    OpenAIRE

    鬼束, 幸樹; 秋山, 壽一郎; 松田, 孝一郎; 寺島, 大輔

    2011-01-01

    Nakamura pointed out that the notch should be placed on one-side compared with alternate to shorten the amount of staying time of fish in a pool. However, there is little information on the suitable orifice location for shorten the amount of staying time of fish in a pool. In this study, orifice location in a pooland-weir fishway is changed alternate or one-side. Behaviors of ayu, Plecoglossus altivelis altivelis, were obtained with the aid of two sets of digital video cameras. It was found t...

  15. Representasi Seksualitas dalam Novel Saman Karya Ayu Utami

    Directory of Open Access Journals (Sweden)

    Rina Patriana Chairiyani

    2012-09-01

    Full Text Available Saman is a literature written by Ayu Utami. Showing a representation of a female counterpart ideology that differs from what has already been made in heterosexual and patriarchal society. The community considers sex is taboo to talk openly. In this novel it is represented openly. Women are no longer in a weak position, and have choices. In this paper, research methodology used is qualitative method.

  16. Servqual Method: Analisis Kualitas Pelayanan Beauty Advisor Sari Ayu Martha Tilaar

    Directory of Open Access Journals (Sweden)

    Ami Widyastuti

    2012-12-01

    Full Text Available Penelitian ini bertujuan untuk menganalisa kualitas pelayanan beauty advisor Sari Ayu Martha Tilaar guna meningkatkan kualitas pelayanan pada konsumen. Pengambilan data menggunakan skala kualitas pelayanan yang disusun berdasarkan metode Servqual dari (Parasuraman, dkk., 1988 pada 72 orang konsumen Sari Ayu Martha Tilaar. Sampel diambil dengan menggunakan teknik insidental random sampling. Analisis data menggunakan pengukuran gap 5 dari metode Servqual. Hasil penelitian menemukan ada kesenjangan pelayanan (service gap antara persepsi dan harapan konsumen sebesar -81.09 dimana nilai minus disini berarti, kinerja kerja beauty advisor Sari Ayu Martha Tilaar berada pada kategori rendah. Kesimpulannya, beauty advisor sebagai garda lini depan dalam pemasaran belum bekerja secara optimal.

  17. MENGENAL LEBIH DEKAT KERAPU BEBEK (Cromileptes altivelis HASIL BUDIDAYA

    Directory of Open Access Journals (Sweden)

    Ida Komang Wardana

    2015-06-01

    Full Text Available Kerapu bebek/kerapu tikus/humpback grouper (Cromileptes altivelis merupakan salah satu komoditas perikanan budidaya laut yang memiliki nilai jual tinggi. Sebelum berkembangnya kerapu hibrid, Balai Besar Penelitian dan Pengembangan Budidaya Laut Gondol, sudah berhasil mengembangkan pembenihan ikan kerapu bebek meskipun sintasan yang diperoleh masih bervariasi. Pemantauan benih hasil budidaya sebagian besar terfokus pada pertumbuhan dan jika sudah mencapai ukuran konsumsi bisa dijual atau diekspor ke Hongkong. Sebagai institusi penelitian nampaknya masih perlu mengupas lebih detail komoditas yang dikembangkan. Beberapa catatan yang dapat diperhatikan, untuk lebih mengenal komoditas kerapu bebek, sebelum dijadikan sebagai ikon jenis ikan yang pernah memiliki masa kejayaan, karena saat ini popularitasnya relatif menurun karena ada larangan ekspor dan masuk dalam satwa CITES. Catatan tersebut antara lain; kerapu bebek selama fase yuwana (2-3 bulan dapat dijadikan sebagai kandidat ikan hias dengan performasi bintik bintik hitam pada permukaan tubuh yang merupakan keunikan tersendiri, karena jumlah dan polanya berbeda antara satu individu dengan yang lainnya serta akan bertambah jumlahnya seiring dengan bertambahnya umur dan bobot badan. Spot/bintik hitam kemungkinan identik dengan sidik jari manusia, yang bisa digunakan untuk membedakan antara satu individu dengan individu lainnya dalam satu populasi. Gonad kerapu bebek turunan pertama (F1 dengan umur dan kisaran bobot badan yang kurang lebih sama (600-900 g, menunjukkan 95% berada pada fase betina. Sementara pematangan dan pembentukan gonad jantan pada kerapu bebek hasil budidaya dapat dipacu dengan rangsangan hormonal dan pemberian pakan segar dengan kualitas yang baik. Dari hasil pengamatan diperoleh informasi bahwa kerapu bebek hasil budidaya dapat memijah dengan baik dan menghasilkan turunan generasi kedua (F2, akan tetapi kualitas telur dan larva yang dihasilkan masih lebih rendah bila

  18. Investigation of Marine Magnetic Vector Anomalies in the Southern Ayu Trough, Southern Philippine Sea

    Science.gov (United States)

    Kim, S.; Lee, S.

    2001-12-01

    The Ayu Trough is a divergent margin, located at the boundary of the Philippine Sea and the Caroline Plates. Previous attempts to resolve the magnetic lineations of this region using total magnetic field have not been successful because it represents a case of east-west spreading center situated near the magnetic equator. The difficulty is compounded by the fact that the Ayu Trough is an ultra-slow-spreading center, which exhibits a complex history of evolution. As an attempt to get around the inherent ambiguity of total field measurement, a shipboard three-component magnetometer was employed during our recent cruise to the Ayu Trough along with proton precession magnetometer. This study examines the vector magnetic data collected in the south part of the Ayu Trough and compares them with tectonic features identified from other geophysical measurements. First, the magnetic field due to the ship was removed from the measured field. We then subtracted the International Geomagnetic Reference Field and the diurnal variation recorded at Guam observatory from our measurement. The amplitude of the north-south component anomalies is substantially less than that of other anomalies, which suggests that the general strike of magnetic lineations in this region is north-south. The magnetic boundary and their strike were estimated by assuming that the magnetic sources are two-dimensional. On the basis of its tectonic structure and interpretation of the total field anomaly pattern, the Ayu Trough can be divided into two sections with distance from the axis: the exterior (> 100 km from the axis) which shows evidence of rifted margin and the interior (appears to be useful in determining major boundaries, such as those between the exterior and interior sections in our area. Within the interior section of the Ayu Trough, however, the discrimination of magnetic boundaries was less successful. The lack of consistency of estimated strikes between track lines suggests that the seafloor

  19. Konflik Batin Tokoh Utama Dalam Novel Saman Karya Ayu Utami: Pendekatan Psikoanalisis Sigmund Freud

    OpenAIRE

    Saputra, Joko

    2015-01-01

    Psikologi dalam sastra mengandung kejadian-kejadian yang tampak lewat perilaku tokoh-tokohnya. Melalui perilaku tokoh-tokohnya akan tampak konflik batin yang dialami oleh masing-masing tokoh dalam karya sastra. Penelitian ini bertujuan untuk mengetahui konflik batin tokoh utama berdasarkan peristiwa-peristiwa yang dialaminya dalam novel Saman Karya Ayu Utami. Untuk memperoleh hasil tersebut dipergunakan teori psikologi sastra dengan penerapan teori-teori psikoanalisis Sigmund Freud. Metode pe...

  20. TARI OLEG TAMULILINGAN GAYA PELIATAN KARYA I GUSTI AYU RAKA RASMI: KREATIVITAS GARAP DAN PEMBELAJARANNYA

    Directory of Open Access Journals (Sweden)

    Ni Komang Tri Paramityaningrum

    2015-05-01

    Full Text Available Tari Oleg Tamulilingan gaya Peliatan adalah sebuah karya seni yang diciptakan oleh I Gusti Ayu Raka Rasmi. Tujuan penelitian untuk mendeskripsikan struktur, kreativitas garap dan proses pembelajaran tari Oleg Tamulilingan gaya Peliatan. Penelitian ini menggunakan metode kualitatif dengan pendekatan interpretatif. Teknik pengumpulan data adalah observasi, wawancara, dan dokumentasi. Teknik analisis data menggunakan model analisis data interaktif dari Miles dan Huberman. Hasil penelitian adalah; (1 Ada tiga struktur tari Oleg Tamulilingan gaya Peliatan seperti papeson, pengawak,dan pekaad; (2 Kreativitas ragam gerak meliputi lima perubahan seperti angsel kado menjadi miles, nyerere menjadi luk nerudut, nyeregseg ngider menjadi nyeregseg meplincer, meipuk-meipuk menjadi mearas-aras, dan nyakup bawa. Kreativitas tata busana dapat diwujudkan dalam bentuk kamen, sabuk prada, oncer, ampok-ampok, tutup dada, gelang kana, badong lanying, gelungan dan udeng. Tata rias wajah meliputi penggunaan eyeshadow yang mencolok dan tidak menggunakan kecek/titik putih di bagian dahi/srinata; (3 Proses pembelajaran yang dilakukan adalah memberikan teknik dan menyuruh peserta didik untuk mempraktekkan tari Oleg Tamulilingan gaya Peliatan dengan pelatih I Gusti Ayu Raka Rasmi.  Oleg Tamulilingan dance of Peliatan style is an art work created by I Gusti Ayu Raka Rasmi. The aimed was to describe the structure, the learning and analyze the creativity of the dance. The aimed of the research was to describe the structure, the learning of Oleg Tamulilingan dance of Peliatan style and analyze the creativity of Oleg Tamulilingan dance of Peliatan style created I Gusti Ayu Raka Rasmi . The study used qualitative method through interpretative approach. Technique of collecting the data used observation, interview, documentation and recording. The data analysis used Miles and Huberman qualitative data analysis model. The result was; (1 Three strucutres of the dance such as papesan

  1. The reformasi of Ayu Utami; Attacking the monopoly of the great religions

    Directory of Open Access Journals (Sweden)

    Karel Steenbrink

    2015-07-01

    Full Text Available In eight novels, Ayu Utami has presented critical attacks on doctrines and practices of the major religions in Indonesia. The two books, that describe the spiritual struggle of the Catholic priest Saman (1998–2002, call for a religion that is more active in the political arena, but leaves sexual rules to the individual people. The novel Bilangan Fu (2008 condemns the monopoly of the great religions in favour of local and individual spirituality. This is developed in a series of novels of which two more have already appeared. A third cycle of three more or less autobiographic novels (2003–2013 sketch her personal quest from atheism towards a critical but positive spirituality condemning a clerical and monopolist trend in Catholicism. Utami’s criticism of the great religions is external (more players in the field should be recognised and internal (religious leaders should have more modest claims towards their faithful and leave more space for personal choice.

  2. Rhizospheric Microflora Escalating Aroma Constituents and Yield Attributes in Ocimum tenuiflorum (L. cv. CIM-Ayu

    Directory of Open Access Journals (Sweden)

    Shilpi Khare Saikia

    2014-01-01

    Full Text Available The exploration of rhizospheric microbial flora for crop yield enhancement is well established. Rhizospheric microbes influence the plant physiology by imparting several beneficial effects, namely, Nitrogen fixation, increased nutrient uptake, and secondary metabolites production on their host plants. The present study investigates the response of Bacillus megaterium ATCC No. 13525, Pseudomonas fluorescens ATCC No. 14581, and Trichoderma viride MTCC No. 167 in alone and combined treatments for their effect on growth and yield parameters in a commercially important Ocimum tenuiflorum L. cv. CIM-Ayu. The plant is therapeutically important for its essential oil constituents, namely, eugenol, β-caryophyllene, and various monoterpenes. The combination treatments, T7 (B. megaterium + P. fluorescens and T8 (B. megaterium + P. fluorescens + T. viride, showed maximum enhancement (27.27% of percentage essential oil as compared to untreated control. Nutrient uptake especially N2 content was significantly increased (43% with the treatment T8 (B. megaterium + P. fluorescens + T. viride. Amongst major essential oil constituents, eugenol content was maximally increased by 58.5% as compared to 42.9% (control indicating a cumulative role of microbial inoculants for crop yield boost-up.

  3. Chronic hydrocarbon exposure of harlequin ducks in areas affected by the Selendang Ayu oil spill at Unalaska Island, Alaska

    Science.gov (United States)

    Flint, Paul L.; Schamber, J.L.; Trust, K.A.; Miles, A.K.; Henderson, J.D.; Wilson, B.W.

    2012-01-01

    We evaluated chronic exposure of harlequin ducks (Histrionicus histrionicus) to hydrocarbons associated with the 2004 M/V Selendang Ayu oil spill at Unalaska Island, Alaska. We measured levels of hepatic 7-ethoxyresorufin-O-deethylase activity (EROD) in liver biopsy samples as an indicator of hydrocarbon exposure in three oiled bays and one reference bay in 2005, 2006, and 2008. Median EROD activity in ducks from oiled bays was significantly higher than in the reference bay in seven of nine pairwise comparisons. These results indicated that harlequin ducks were exposed to lingering hydrocarbons more than three years after the spill.

  4. THE EFFECTS OF CRUDE RECOMBINANT VIRAL PROTEIN VACCINES AGAINST GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV ON HUMPBACK GROUPER (Cromileptes altivelis

    Directory of Open Access Journals (Sweden)

    Ketut Mahardika

    2015-12-01

    Full Text Available Infection of Megalocytivirus cause serious mass mortality in marine fish in South East Asian countries. The aim of this study was to produce recombinant of GSDIV capsid protein and its protection to humpback grouper Cromileptes altivelis against grouper sleepy disease iridovirus (GSDIV. A major capsid protein (MCP was selected for use as a crude subunit vaccines. This gene target (MCP was inserted to the protein expression system vector of pET SUMO and cloned in cells bacteria Escherichia coli strain BL-21. The MCP was succeded to be induced using 1 mM of IPTG. Results of protein analysis using MALDI TOF-TOF indicated that the MCP has measurement of 49.566 kDa with PI index of 6.00, and contained 453 amino acids. BLAST homology analysis exhibited that the amino acid sequence of the MCP showed high similarity with MCP of Red Sea Bream Iridovirus (RSIV. E. coli expressing MCP protein was inactivated using 0.03% formalin overnight and washed using PBS. The inactivated E. coli as a crude subunit vaccine was then injected intramuscularly to humpback grouper juveniles. Subsequently, the juveniles were challenged tested with GSDIV. The juveniles vaccinated with the MCP recombinant bacteria showed significantly higher survival rates than control those vaccinated with PBS. Thus, the MCP fusion protein is considered as a potential vaccine against GSDIV infections in grouper.

  5. Modes of transmission of Glugea plecoglossi (Microspora) via the skin and digestive tract in an experimental infection model using rainbow trout, Oncorhynchus mykiss (Walbaum).

    Science.gov (United States)

    Lee, S-J; Yokoyama, H; Ogawa, K

    2004-08-01

    Glugea plecoglossi (Microspora) is a significant cause of economic loss in cultured ayu, Plecoglossus altivelis, in Japan, due to the unsightly appearance of infected fish harbouring xenomas in the body cavity. Modes of transmission of G. plecoglossi via the skin and digestive tract were studied in an experimental infection model using rainbow trout, Oncorhynchus mykiss. Combined with Uvitex 2B and in situ hybridization (ISH) assays, the early development of G. plecoglossi was successfully traced. Following a bath exposure of fish Uvitex 2B-labelled G. plecoglossi spores were observed to attach to microscopic injuries (trypan blue-positive sites) of fish skin, after which ISH-positive sporoplasms were found to invade the epidermis as early as 5 min post-infection (PI), migrating rapidly to the subdermis. It was also shown that G. plecoglossi entering via the skin does not spread into the internal organs but develops into subdermal xenomas. After rainbow trout were exposed to G. plecoglossi spores by oral intubation, spores germinated in the intestinal lumen, followed by penetration of sporoplasms into the gut mucosal epithelium 5 min PI. In vitro trials determining stimulation factors (fish mucus, changes in pH, digestive enzymes) for the extrusion of the polar tube were inconclusive. The present study indicates that skin wounds and the gut epithelium can be portals of entry of G. plecoglossi and that natural infection in fish seems to occur perorally rather than via the skin.

  6. Status and future perspectives of vaccines for industrialised fin-fish farming.

    Science.gov (United States)

    Brudeseth, Bjørn Erik; Wiulsrød, Rune; Fredriksen, Børge Nilsen; Lindmo, Karine; Løkling, Knut-Egil; Bordevik, Marianne; Steine, Nils; Klevan, Are; Gravningen, Kjersti

    2013-12-01

    Fin fish farming is developing from extensive to intensive high industrial scale production. Production of fish in high-density growth conditions requires effective vaccines in order to control persistent and emerging diseases. Vaccines can also have significant positive impact on the reduced usage of antibiotics. This was demonstrated when vaccines were introduced in Norway for Atlantic salmon (Salmo salar) in the late eighties and early nineties, resulting in a rapid decline of antibiotics consumption. The present review will focus on current vaccine applications for farmed industrialized fish species such as Atlantic salmon, coho salmon (Oncorhynchus kisutch), rainbow trout (Oncorhynchus mykiss), ayu (Plecoglossus altivelis), cod (Gadus morhua), sea bass (Dicentrarchus labrax), gilt-head sea bream (Sparus aurata), yellowtail (Seriola quinqueradiata), great amberjack (Seriola dumerili), barramundi (Lates calcarifer), japanese flounder (Paralichythys olivaceus), turbot (Scophthalmus maximus), red sea bream (Pagrus major), rock bream (Oplegnathus fasciatus), seven band grouper (Epinephelus septemfasciatus), striped catfish (Pangasianodon hypophthalmus), channel catfish (Ictalurus punctatus) and tilapia (Oreochromis niloticus). This paper will review the current use of licensed vaccines in fin fish farming and describe vaccine administration regimes including immersion, oral and injection vaccination. Future trends for inactivated-, live attenuated - and DNA - vaccines will also be discussed.

  7. Complete genome sequence analysis of two Pseudomonas plecoglossicida phages, potential therapeutic agents.

    Science.gov (United States)

    Kawato, Yasuhiko; Yasuike, Motoshige; Nakamura, Yoji; Shigenobu, Yuya; Fujiwara, Atushi; Sano, Motohiko; Nakai, Toshihiro

    2015-02-01

    Pseudomonas plecoglossicida is a lethal pathogen of ayu (Plecoglossus altivelis) in Japan and is responsible for substantial economic costs to ayu culture. Previously, we demonstrated the efficacy of phage therapy against P. plecoglossicida infection using two lytic phages (PPpW-3 and PPpW-4) (S. C. Park, I. Shimamura, M. Fukunaga, K. Mori, and T. Nakai, Appl Environ Microbiol 66:1416-1422, 2000, http://dx.doi.org/10.1128/AEM.66.4.1416-1422.2000; S. C. Park and T. Nakai, Dis Aquat Org 53:33-39, 2003, http://dx.doi.org/10.3354/dao053033). In the present study, the complete genome sequences of these therapeutic P. plecoglossicida phages were determined and analyzed for deleterious factors as therapeutic agents. The genome of PPpW-3 (myovirus) consisted of 43,564 bp with a GC content of 61.1% and 66 predicted open reading frames (ORFs). Approximately half of the genes were similar to the genes of the Escherichia coli phage vB_EcoM_ECO1230-10 (myovirus). The genome of PPpW-4 (podovirus) consisted of 41,386 bp with a GC content of 56.8% and 50 predicted ORFs. More than 70% of the genes were similar to the genes of Pseudomonas fluorescens phage ϕIBB-PF7A and Pseudomonas putida phage ϕ15 (podoviruses). The whole-genome analysis revealed that no known virulence genes were present in PPpW-3 and PPpW-4. An integrase gene was found in PPpW-3, but other factors used for lysogeny were not confirmed. The PCR detection of phage genes in phage-resistant variants provided no evidence of lysogenic activity in PPpW-3 and PPpW-4. We conclude that these two lytic phages qualify as therapeutic agents.

  8. Developmental and Morphological Characteristics of Embryo, Larval, Juvenile, and Young Fish, Chromileptes altivelis%驼背鲈(Chromileptes altivelis)的胚胎发育及仔、稚、幼鱼形态观察

    Institute of Scientific and Technical Information of China (English)

    张梦淇; 陈超; 李炎璐; 贾瑞锦; 于宏; 梁友; 蔡春有; 庞尊方

    2014-01-01

    Artificial breeding of Chromileptes altivelis is difficult to marine fishing, and related studies on C. altivelis are limited. The present study investigated the embryonic and morphological development of larval, juvenile and young fish of C. altivelis using microscope, anatomical lens and digital camera. The morphological features and the time of development stages were described. The results showed that the embryonic development of C. altivelis was divided into five stages:cleavage stage, blastula stage, gastrula stage, neurula stage, and organogenesis stage. The embryonic development requires 27 h 25 min under the following condition in the sea water at 25−26℃ and salinity of 30. The post embryonic development of C. altivelis was divided into larval stage, juvenile stage and young fish stage based on the features of the yolk-sac, finfold, second dorsal fin spine, pelvic fin spine, scale, and body color. The larval stage was further classified into early larvae and late larvae based on the existence of the yolk-sac or not. The embryos developed into prelarvae in 2 days under water environment with temperature between 22 and 26℃, salinity between 29 and 31, DO≥5 mg/L. The yolk-sac of larvae disappeared and the postlarvae began at day 3. The larvae started to develop to juveniles at day 31. The juveniles completed metamorphosis and became young fish at day 57. The growth and configuration of the second spine of dorsal fin and the first spine of ventral fin were the most obvious changes during early development of C. altivelis. This study provides information for the propagation, seed culture and morphological identification of grouper fish.%对驼背鲈(Chromileptes altivelis)的胚胎发育及仔、稚、幼鱼的形态特征进行了详细的观察与研究,描述了从受精卵到仔、稚、幼鱼各发育期的时间和形态特征变化。结果表明,在水温25−26℃、盐度30的海水中,受精卵历时27 h 25 min完成整个胚胎发育

  9. Histological and histochemical studies on digestive system of Cromileptes altivelis%驼背鲈消化系统组织学与组织化学研究

    Institute of Scientific and Technical Information of China (English)

    区又君; 勾效伟; 李加儿

    2011-01-01

    The digestive system of Cromileptes altivelis was studied by dissection, histology and histochemistry with HE and AB-PAS dyeing. Results indicated that many broad mucosa pleats existed on the internal surface of oesophagus. The stomach was composed of cardiac portion, blind sac and pyloric portion, among which gastric pits and gastric gland in cardiac portion were the most in number and the blind sac was the thickest. There were abundant mucosa pleats on the surface of foregud, midgut and hidgut where the density of mucus cell was the maximum. 10-13 pyloric caeca were found existing in the juncture of pyloric portion and foregut, with the similar microstructure to foregut. The type II cells which excreted neutral mucopolysaccharide were the major mucous cells in oesophagus, stomach, foregut, midgut, hindgut and pyloric caeca. The feature was characterized by the adaptive relationship between microstructure of the digestive system and carnivorous habit in this species.%采用形态解剖、组织学及组织化学方法,分别用HE和AB-PAS染色法对驼背鲈(Cromileptes altivelis)的消化系统进行了研究.结果表明:驼背鲈食道内表面分布较多宽大的粘膜褶;胃分为贲门部、盲囊部和幽门部,贲门部胃小凹和胃腺最多,盲囊部厚度最大;前、中、后肠粘膜褶丰富,后肠粘液细胞密度最大;幽门与前肠交界处有幽门盲囊10~13个,结构与前肠相似.食道,胃,前、中、后肠及幽门肓囊均以分泌中性粘多糖的Ⅱ型粘液细胞为主.驼背鲈消化系统的结构表现出与其肉食性习性相适应的特点.

  10. Modeling the potential spread of the recently identified non-native panther grouper (Chromileptes altivelis in the Atlantic using a cellular automaton approach.

    Directory of Open Access Journals (Sweden)

    Matthew W Johnston

    Full Text Available The Indo-pacific panther grouper (Chromileptes altiveli is a predatory fish species and popular imported aquarium fish in the United States which has been recently documented residing in western Atlantic waters. To date, the most successful marine invasive species in the Atlantic is the lionfish (Pterois volitans/miles, which, as for the panther grouper, is assumed to have been introduced to the wild through aquarium releases. However, unlike lionfish, the panther grouper is not yet thought to have an established breeding population in the Atlantic. Using a proven modeling technique developed to track the lionfish invasion, presented is the first known estimation of the potential spread of panther grouper in the Atlantic. The employed cellular automaton-based computer model examines the life history of the subject species including fecundity, mortality, and reproductive potential and combines this with habitat preferences and physical oceanic parameters to forecast the distribution and periodicity of spread of this potential new invasive species. Simulations were examined for origination points within one degree of capture locations of panther grouper from the United States Geological Survey Nonindigenous Aquatic Species Database to eliminate introduction location bias, and two detailed case studies were scrutinized. The model indicates three primary locations where settlement is likely given the inputs and limits of the model; Jupiter Florida/Vero Beach, the Cape Hatteras Tropical Limit/Myrtle Beach South Carolina, and Florida Keys/Ten Thousand Islands locations. Of these locations, Jupiter Florida/Vero Beach has the highest settlement rate in the model and is indicated as the area in which the panther grouper is most likely to become established. This insight is valuable if attempts are to be made to halt this potential marine invasive species.

  11. 驼背鲈脑垂体显微和超微结构的观察%Microstructural and ultrastructural study of pituitary in highfin grouper Cromileptes altivelis

    Institute of Scientific and Technical Information of China (English)

    王静香; 李加儿; 区又君; 王刚

    2011-01-01

    采用PAS、Mallory三色法和腺垂体不同细胞改良染色法3种组织学染色方法,以及透射电镜技术,对驼背鲈(Cromileptes altivelis)脑垂体的形态、组织结构和超微结构进行了研究.结果表明,驼背鲈脑垂体为圆球形实心腺体,位于间脑腹面,由神经垂体和腺垂体2部分组成.根据神经纤维中所含分泌颗粒形态和大小把神经分泌纤维分为A1、A2和B型.神经纤维中存在2种垂体细胞,即呈椭圆形的Ⅰ型垂体细胞和呈梭形的Ⅱ型垂体细胞.腺垂体由3部分组成:前外侧部(RPD)、中外侧部(PPD)和中间部(PI).腺垂体部可以鉴别出6种内分泌细胞,其中RPD有2种内分泌细胞:促肾上腺皮质激素(ACTH)分泌细胞和催乳激素(PRL)分泌细胞;PPD有3种内分泌细胞:生长激素(GH)分泌细胞、促性腺激素(GTH)分泌细胞和促甲状腺激素(TSH)分泌细胞;PI只有1种内分泌细胞,即促黑激素(MSH)分泌细胞.此外,腺垂体还有1种非分泌细胞星状细胞(SC).驼背鲈腺垂体中6种内分泌细胞的分布情况为:前外侧部分布有2种细胞,即嗜酸性的PRL分泌细胞和嗜碱性的ACTH分泌细胞;中外侧部有GH、GTH、TSH 3种分泌细胞;MSH分泌细胞分布在中间部.驼背鲈脑垂体中只有1种GTH分泌细胞,只是呈多种形态.本研究有助于进一步了解鱼类脑垂体的结构,为驼背鲈的繁育和养殖提供理论依据.%Pituitary was one of most complicated endocrine gland, the function of it was diversification. Pituitary could modulate the activities of the body organs and tissues. It was necessary to investigate structure offish pituitary in order to provide the basis for histological morphology, reproductive biology and pathology. Using improved staining, Mallory and PAS staining method, and transmission electron microscopy technique, the microstructure and the ultrastructure of the pituitary of Cromileptes altivelis was examined. Results showed that: the pituitary of the C

  12. Ultrastructure of peripheral blood cells of highfin grouper Cromileptes altivelis%驼背鲈血细胞的超微结构

    Institute of Scientific and Technical Information of China (English)

    王静香; 李加儿; 区又君; 王永翠

    2011-01-01

    利用电镜技术对驼背鲈(Cromileptes altivelis)外周血细胞的超微结构进行了研究.结果表明,电镜下可区分6种类型的细胞:红细胞、粒细胞、单核细胞、淋巴细胞、血栓细胞和浆细胞.红细胞呈圆形或椭圆形,可见线粒体、少数囊泡;根据细胞中的颗粒形态大小和细胞核的形态,可将粒细胞分为4种类型:Ⅰ型粒细胞、Ⅱ型粒细胞、Ⅲ型粒细胞和Ⅳ型粒细胞.单核细胞圆形,表面较平整,偶有伪足伸出,空泡多见;淋巴细胞的胞质少,仅在细胞核边缘处形成薄薄的一层,细胞器少;血栓细胞异染色质丰富,沿核膜呈带状分布,胞质中有较多大小不等的空泡和少量的线粒体;浆细胞中粗面内质网成层分布包绕在核周围.此外可见到嗜曙红细胞吞噬红细胞、血栓细胞成群聚集分布的现象.%Using electron microscopy, we studied the ultrastructure of peripheral blood cells of Cromileptes allivelis. Six types of cells ( erythrocyte, granulocyte, monocyte, lymphocyte, thrombocyte and plasma cell) were detected under the electron microscope. Eryth rocyte is round or oval with visible mitochondria and a few vesicles. According to the size, shape of particles and form of nucleus, gran ulocytes can be divided into 4 types: Ⅰ-type granulocyte, Ⅱ-type granulocyte, Ⅲ-type granulocyte and Ⅳ-type granulocyte. Monocyte is round with smooth surface and vacuoles, occasionally holding out pseudopodia. Lymphocyte has a few organelles and little cytoplasm which only forms a thin layer around the nucleolus. Thrombocyte is rich in heterochromatins which distribute zonally along the nuclear membrane. There are many vacuoles at various sizes and a small amount of mitochondria. Rough endoplasmic reticulum distributes hi erarchically around the nucleolus in plasma cell. In addition, erythrocytes could be seen to be phagocytized by eosinophils and throm bocytes gathered in groups.

  13. Morphology and histology of head-kidney and spleen in Cromileptes altivelis%驼背鲈头肾和脾脏的形态组织学观察

    Institute of Scientific and Technical Information of China (English)

    区又君; 刘江华; 李加儿; 吴水清; 谢木娇

    2015-01-01

    [目的]观察研究驼背鲈(Cromileptes altivelis)头肾和脾脏的组织学结构,以充实该鱼类免疫器官的比较解剖学、组织学等基础资料,为深入研究驼背鲈生物学及其养殖学提供理论依据.[方法]常规采集驼背鲈的头肾和脾脏,经石蜡切片及HE染色后,光学显微镜下观察头肾和脾脏的显微组织结构.[结果]驼背鲈头肾位于食道背面、胸腔内咽退缩肌之上,其外覆盖一层疏松结缔组织性被膜,属于淋巴样实质组织,无肾单位及明显小梁,分为红细胞聚集区和白细胞聚集区,其实质主要由淋巴组织、血管和血窦组成,其间可见黑色素巨噬细胞中心.驼背鲈脾脏位于胃后方、肠的前部背面,表面也覆盖一层较薄的结缔组织性被膜,其实质由红髓和白髓混合排列组成,小梁不明显,含有大量的淋巴细胞、血细胞及粒细胞,有椭圆体和黑色素巨噬细胞中心,脾脏中的动脉、静脉及神经束互相结伴而行.[结论]驼背鲈头肾是机体重要的造血和免疫器官,而脾脏具有很强的血液过滤及储存功能.

  14. MONOGENOIDEA OF CHINESE MARINE FISH Ⅻ.ONE NEW SPECIES OF GENUS NEOHALIOTREMA FROM ABUDEFDUF SP. AND ONE NEW RECORD OF GENUS HALIOTREMA FROM CROMILEPTES ALTIVELIS%中国海水鱼类单殖吸虫研究Ⅻ新海盘虫属一新种及海盘虫属一新记录

    Institute of Scientific and Technical Information of China (English)

    李海燕; 李桂峰

    2000-01-01

    报道了寄生于豆娘Abudefduf sp.鳃上的新海盘虫属一新种和寄生于驼背鲈Cromileptes altivelis 鳃上的海盘虫属一新记录.双曲片新海盘虫,新种 Neohaliotrema dicurvilamellatum, sp.nov.与Neohaliotrema bychowskii Zhukov,1976相似,但支持器的形态结构不同和交接管量度上有差异.驼背鲈海盘虫Haliotrema cromileptis Young,1968与原始描述相比,除交接管稍长外,其它量度与形态基本一致.

  15. REPRESENTASI SEJARAH INDONESIA DALAM NOVEL-NOVEL KARYA AYU UTAMI

    Directory of Open Access Journals (Sweden)

    Wiyatmi Wiyatmi Wiyatmi

    2013-12-01

    (ethe demonstration by students of Technology Institute of Bandung and the publication of the Student Struggle White Book in 1978. Second, the events are represented in parts integrated into the events that the characters experience. Historical events from factual events are contextualized in the novels. In the New Historicism perspective, socio-political historical events are presented to question historical truths previously recorded.

  16. Substitution of fishmeal with soybean meal in humpback Grouper, Cromileptes altivelis juvenile diets supplemented with phytase

    Directory of Open Access Journals (Sweden)

    Rachman Syah

    2006-12-01

    Full Text Available Feeding experiments were conducted to evaluate the effects of replacing fishmeal with soybean meal in diet on growth of humpback grouper. Fifteen cages of 1 x 1 x 1.2 m3 each stocked with 16 humpback grouper juveniles (61.3 ± 0.4 g/pc were set up randomly in seawater. Fish were fed to satiation twice daily for 112 days. The control diet contained 61.9% fishmeal (63.34% crude protein. Four isonitrogenous (48% crude protein and isocaloric (4.7 kcal/g feed diets supplemented with commercial phytase “Rhonozyme-P” at 0.075% were formulated to contain different levels (8%, 16%, 24%, and 32% soybean meal (43.65% crude protein as a partial replacement for fishmeal. These diets contained total phosphorus levels between 3.6—4.5 (±0.4 % and 0.7—1.5 (±0.04 % available phosphorus. Replacement of fishmeal with soybean meal (8 to 32% replacement was not significantly different (P>0.05 to the control diet on daily growth rate (DGR, food conversion ratio (FCR, protein efficiency ratio (PER, and daily food consumption (DFC. However, the dietary levels of soybean meal significantly affected (P<0.05 whole body protein and phosphorus retention (Table 1. These data suggest that addition of phytase in diets could improve protein and phosphorus availability and reduce nitrogen and phosphorus loading in the environment. Phytase can therefore play an important role in formulating eco-friendly feed for humpback grouper. Based on P loading, supplementation of phytase enable up to 24% fishmeal replacement with soybean meal.

  17. QUANTITATIVE HISTOPATHOLOGICAL ANALYSIS OF ENLARGED CELLS DERIVED FROM HUMPBACK GROUPER, Cromileptes altivelis INFECTED WITH GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV

    Directory of Open Access Journals (Sweden)

    Indah Mastuti

    2010-12-01

    Full Text Available Pathognomonic sign of grouper sleepy disease iridovirus (GSDIV was proposed to be the formation of enlarged cells and necrotic cells, in which under electron microscope, it is revealed to be the inclusion body bearing cells (IBCs and necrotic cells containing GSDIV viral particles. Spleen and kidney tissues are the major sites for formation of enlarged cells. This paper described the result of histopatological analysis of enlarged cells found in the spleen and kidney of moribund fish after GSDIV challenge. A pathogenicity test was conducted on fish stocked in two tanks for infected groups and the other two tanks for uninfected control groups (15 fish per tank. The infected groups were injected intramuscularly with 0.1 ml of the viral inoculum. The uninfected groups were injected with the same amount of EMEM-2. The GSDIV-infected humpback grouper began to die after 6 days post infection and all died after 7 dpi, excluding one fish which had survived until the end of experimental infection periods (93% to 100% mortality. All of the diseased fish showed massive formation of enlarged cells in their spleen, head kidney and trunk kidney. The largest number of enlarged cells was observed on head kidneys and subsequently followed by spleens, trunk kidney (2.0-200.3/field of view. This result suggested that the number of enlarged cells in the affected organs was not the direct factor that led to the mortality of fish.

  18. Development of a spatially distributed model of fish population density for habitat assessment of rivers

    Science.gov (United States)

    Sui, Pengzhe; Iwasaki, Akito; Ryo, Masahiro; Saavedra, Oliver; Yoshimura, Chihiro

    2013-04-01

    discharge (MID) was found to be positively correlated to 9 among 16 fish species. For duration of high and low flows (DHF and DLF), longer DHF/DLF was corresponded to lower population density for 7/6 fish species, respectively, such as Rhinogobius kurodai and Plecoglossus altivelis altivelis. Among physical habitat conditions, sinuosity index (SI, the ratio between actual river section length and straight line length) seems to be the most important parameter for fish population density in Sagami River basin, since it affects 12 out of 16 fish species, followed by mean longitudinal slope (S) and number of downstream dams (NLD). Above results demonstrated the applicability of fish distribution model to provide quantitative information on flow conditions required to maintain fish population, which enabled us to evaluate and project ecological consequences of water resource management policy, such as flood management and water withdrawal.

  19. Radiological impact of the nuclear power plant accident on freshwater fish in Fukushima: An overview of monitoring results.

    Science.gov (United States)

    Wada, Toshihiro; Tomiya, Atsushi; Enomoto, Masahiro; Sato, Toshiyuki; Morishita, Daigo; Izumi, Shigehiko; Niizeki, Kouji; Suzuki, Shunji; Morita, Takami; Kawata, Gyo

    2016-01-01

    Radionuclide ((131)I, (134)Cs, and (137)Cs) concentrations of monitored freshwater fish species collected from different habitats (rivers, lakes, and culture ponds) in Fukushima Prefecture during March 2011-December 2014 (total 16 species, n = 2692) were analyzed to present a detailed description of radionuclide contamination after the Fukushima Dai-ichi Nuclear Power Plant (FDNPP) accident, and to elucidate species-specific spatiotemporal declining trends of (137)Cs concentration for their respective habitats. Low concentrations of (131)I (≤24 Bq kg(-1)-wet) were detected from only 11 samples collected during March-June 2011, demonstrating that (131)I transferred to freshwater fish were not intense. In river and lake fishes, a more gradual decrease and higher radiocesium ((134)Cs, (137)Cs) concentrations were observed than in culture pond fishes, which strongly implied that radiocesium in freshwater fish species was mainly bioaccumulated through the food web in the wild. During 2011-2014, percentages above the Japanese regulatory limit of 100 Bq kg(-1)-wet for radiocesium in river and lake fish (14.0% and 39.6%, respectively) were higher than in monitored marine fish (9.9%), indicating longer-term contamination of freshwater fish species, especially in lakes. Higher radiocesium concentrations (maximum 18.7 kBq kg(-1)-wet in Oncorhynchus masou) were found in the northwestern areas from the FDNPP with higher deposition. However, radiocesium contamination levels were regarded as 1-2 orders of magnitude less than those after the Chernobyl accident. Lagged increase of (137)Cs concentration and longer ecological half-lives (Teco: 1.2-2.6 y in the central part of Fukushima Prefecture) were observed in carnivorous salmonids (O. masou, Salvelinus leucomaenis), whereas a rapid increase and decrease of (137)Cs concentration and shorter Teco (0.99 and 0.69 y) were found in herbivorous and planktivorous osmerids (Plecoglossus altivelis, Hypomesus nipponensis) with

  20. 草鱼BPI/LBP基因的克隆及特征研究%Cloning and characterization of BPI/LBP gene from grass carp,Ctenopharyngodon idella

    Institute of Scientific and Technical Information of China (English)

    杨春荣; 苏建国; 黄腾; 彭丽敏

    2011-01-01

    【目的】克隆草鱼杀菌通透性增加蛋白/脂多糖结合蛋白(Bactericidal permeability-increasing pro-tein/LPS-binding protein,BPI/LBP)基因的cDNA全长,分析其结构特征,研究其表达规律,为进一步探明其功能奠定基础。【方法】首先用同源克隆及快速扩增cDNA末端技术(RACE),克隆草鱼BPI/LBP基因的cDNA全长,然后用生物信息学方法分析BPI/LBP基因的结构特征,最后用半定量RT-PCR检测BPI/LBP基因在草鱼不同组织(血、脑、眼%【Objective】 The full-length cDNA sequence of bactericidal permeability-increasing protein/LPS-binding protein(CiBPI/LBP) from grass carp,Ctenopharyngodon idella was cloned to study the characterization and expression profile and to lay a foundation for the functional studies.【Methods】 The CiBPI/LBP cDNA was identified from grass carp gill by homologous cloning and rapid amplification cDNA ends(RACE).The structural characterization was analyzed by bioinformatics method.The CiBPI/LBP expression pattern was examined in different tissues(blood,brain,eye,foregut,midgut,hindgut,gas blander,gill,head kidney,trunk kidney,heart,liver,muscle,skin and spleen) by semi-quantitative RT-PCR.【Result】 The CiBPI/LBP cDNA was 1 568 bp,encoding 473 amino acid(aa) residues,including signal peptide,BPI1 domain(BPI/LBP/CETP N-terminal domain) and BPI2 domain(BPI/LBP/CETP C-terminal domain).The molecular weight of the deduced protein was 51 551 u,and the isoelectric point 8.69.The amino acid sequence of CiBPI/LBP possessed 90%,73%,73%,and 72% identities with the BPI/LBPs of Cyprinus carpio,Oncorhynchus mykiss,Ictalurus punctatus and Plecoglossus altivelis altivelis respectively.CiBPI/LBP protein firstly clustered with BPI/LBP in Cyprinidae species,Cyprinus carpio in the phylogenetic analysis.CiBPI/LBP mRNA was detected in all the 15 tested tissues by semi-quantitative real-time RT-PCR,high in gill,head kidney and trunck kidney

  1. AcEST: BP921825 [AcEST

    Lifescience Database Archive (English)

    Full Text Available Definition sp|Q6AYU1|MO4L1_RAT Mortality factor 4-like protein 1 OS=Rattus norve...nments: (bits) Value sp|Q6AYU1|MO4L1_RAT Mortality factor 4-like protein 1 OS=Rat...tus ... 112 6e-25 sp|Q5NVP9|MO4L1_PONAB Mortality factor 4-like protein 1 OS=Pongo... 108 9e-24 sp|Q6BT38|EA...lated protein EAF3... 92 1e-18 sp|P60762|MO4L1_MOUSE Mortality factor 4-like protein 1 OS=Mus m... 90 4e-18 sp|Q9UBU8|MO4L1_HUMAN Mor...cetyltransferase MYST1 OS=... 42 0.001 >sp|Q6AYU1|MO4L1_RAT Mortality factor 4-like protein 1 OS=Rattus norv

  2. THE HOLDER CONTINUITY OF A CLASS OF 3-DIMENSION ULTRAPARABOLIC EQUATIONS

    Institute of Scientific and Technical Information of China (English)

    Wang Wendong; Zhang Liqun

    2009-01-01

    We obtained the Cα continuity for weak solutions of a class of ultraparabolic equations with measurable coefficients of the form atu = x(a(x, y, t)axu) + b0(x, y, t)axu + b(x, y, t) ayu,which generalized our recent results on KFP equations.

  3. PENGARUH LINGKUNGAN PENGENDALIAN TERHADAP KINERJA AUDITOR PADA PERWAKILAN BADAN PENGAWASAN KEUANGAN DAN PEMBANGUNAN PROVINSI SULAWESI SELATAN

    OpenAIRE

    AMANDITA, TYARA GALUH

    2015-01-01

    2015 Pengaruh Lingkungan Pengendalian terhadap Kinerja Auditor pada Perwakilan Badan Pengawasan Keuangan dan Pembangunan Provinsi Sulawesi Selatan Effect of Control Environment on Auditor???s Performance at Badan Pengawasan Keuangan dan Pembangunan Representative of South Sulawesi Province TYARA GALUH AMANDITA KASTUMUNI HARTO RATNA AYU DAMAYANTI Tujuan penelitian ini adalah untuk menganalisis pengaruh lingkungan pengendalian yang terdiri dari...

  4. Anexo 1

    OpenAIRE

    2015-01-01

    Anexo de la lectura estadística Corpus de nombres femeninos en las tres parcialidades (237) Aba - ya Achino-ma/Chino-ma Ala - maya Ana - maya Anna Aro - rme Acar - ma Ay - cha Ay sa-ma/ Ayssi-ma Ayu-ma Cadco-ma Cama-sea Canca-ma Caqui-ma Cassi-ma Cayla-ma Cissa Cocha-ma Colqui-ma Concha Acussa - na Agua - ya Alu - biça Anca - ma Aqui - ma Ata - ma Auca- rmi Ayra- ma Ayssa-rme Ayu-so Cala-ma Cana-ma Capaco-ma Cara-ma Cassi-maya Cayo Coca Cochi-ta Conado Conega? Acha - ma/Ocha-ma Aha – lla Ana ...

  5. FENOMENA SEKS DALAM NOVEL INDONESIA MUTAKHIR KARYA PENGARANG PEREMPUAN: KAJIAN KRITIK SASTRA FEMINIS

    OpenAIRE

    Wiyatmi

    2012-01-01

    This research is aimed at describing and understanding (1) homo­sexuality phenomena in the current Indonesian novels, (2) the relationship between homosexuality phenomena with the existence of homosexual community in Indonesia, and(3) the relationship between homosexuality phenomena with feminist school. To achieve these aims, six novels are analyzed. They are (1) Larung by Ayu Utami, (2) Supernova by Dee (Dewi) Lestari, (3) Mahadewi Mahadewi (4) Garis Tepi Seorang Lesbian by Herlinati...

  6. Progress in live food production and use in fish and shellfish hatcheries

    OpenAIRE

    Sorgeloos, P

    1998-01-01

    Different species of microalgae, the rotifer, Brachionus spp., and the brine shrimp, Artemia spp., are essential live food in the farming of several species of molluscs (e.g. oyster, clam, scallop), crustaceans (e.g. marine shrimp, freshwater prawn, crabs) and fish (e.g. bass, bream, grouper, turbot, flounder, halibut, ayu). A brief overview is given of the latest developments in their production and use in industrial hatcheries.

  7. “What do Ayurveda Postgraduate Entrance Examinations actually assess?” – Results of a five-year period question-paper analysis based on Bloom's taxonomy

    OpenAIRE

    Deepti Singh; Piyush Kumar Tripathi; Kishor Patwardhan

    2016-01-01

    Background: The standards of Ayurveda education in India are being questioned in the recent years and many suggestions related to educational reforms are being put forth by educators and health policy experts. However, the Post Graduate Entrance Examinations (PGEEs) that are carried out to select the candidates to pursue postgraduate programs have received little attention in this context. Objectives: The objective of this study was to classify the Multiple Choice Questions (MCQs) from Ayu...

  8. Industrial Aquaculture of Famous and Expensive Marine Fishes%名贵海水鱼类工厂化养殖试验研究

    Institute of Scientific and Technical Information of China (English)

    颉晓勇; 李纯厚; 许忠能; 张汉华; 陈利雄; 朱长波; 陈素文

    2011-01-01

    工厂化养殖是未来水产养殖可持续发展的主要养殖模式.在工厂化车间养殖条件下,共放养驼背鲈60 000尾、波纹唇鱼2 600尾,进行名贵海水鱼类养殖试验.结果表明:驼背鲈养殖成活率30.8%,波纹唇鱼养殖成活率81.9%.试验证实工厂化车间养殖系统可以用于名贵海水鱼类驼背鲈和渡纹唇鱼的养殖生产.%Industrial aquaculture is the main aquaculural mode for the sustainable development of aquaculture in future. Under the industrial workshop aquacultural conditions, 60 000 Chromileptes ok we Us and 2 600 Cheiiinus undulates were stocked to conduct industrial aquaculture experiment for famous and expensive marine fishes. The resuIts showed that the survival rates of stocked Chromileptes altivelis and Cheiiinus undulates were 30.8% and 81.9%, respectively. Therefore, this study proved that industrial workshop aquacultural system can be used in aquaculture of famous and expensive marine fishes {Chromileptes altivelis and Cheiiinus undulates).

  9. Influencia de la reposición coronal del margen gingival en las interfases adhesivas generadas tras la cementación de incrustaciones de composite

    OpenAIRE

    Silva Gonçalves, Dayana Da

    2016-01-01

    Tesis Doctoral leída en la Universidad Rey Juan Carlos de Madrid en 2016. Directoras de la Tesis: Laura Ceballos García y María Victoria Fuentes Fuentes Actualmente, los pequeños defectos en los dientes posteriores se obturan con restauraciones directas. El material de elección es la resina compuesta que ha sustituido casi por completo a la amalgama dental. Este cambio permite realizar restauraciones estéticas, sumado a la adopción de enfoques de mínima intervención, lo que ayu...

  10. Aplicación de la psicología positiva para la mejora del clima escolar

    OpenAIRE

    2016-01-01

    Este trabajo de investigación pretende demostrar los beneficios de la denominada psicología positiva, concretada en los conceptos de atención plena y fortalezas personales, en la mejora del clima en el aula y en el rendimiento académico. Para ello repasa la literatura existente al respecto, estudiando los programas ya implantados en otros centros fuera y dentro de nuestro país y hace uso y analiza los resultados de la aplicación del programa MyBullying para detectar alumnos necesitados de ayu...

  11. ANDIR AS A SCARED DANCE AT TISATA VILLAGE, KERAMBITAN, TABANAN REGENCY

    Directory of Open Access Journals (Sweden)

    Ni Made Arshiniwati

    2014-08-01

    Full Text Available Tariandir in the village Tista is a form of Legong Kraton tarisejenis the Tista community called andir.Strukturkoreografinyasangatdekatdengantarilegongkeraton , tetapitari Andir only flourish in the village Tista Kerambitan.Tari inidifungsikan as guardian and bebali art that in the play always involves Rangda sungsungan society , baikditampilkansebagaibagiandariceritamaupunhanyasebagai " witness " pementasannya . Qualitative research by applying the theory of representation , ideology theory , aesthetic theory , and the theory of semiotics examines the main problems that dance andirsebagai sacred art in the village Tista Kerambitan . The results showed that as the art sakraltari Andir related to : ( 1 the use of equipment ( sacred objects in the form of engagement rangdasungsungan ( Ratu Ayu Lingsir and Ratu Ayu Anom , yangdipercayamemilikikekuatanmagis which dapatmelindungimasyarakatTista ; ( 2 any activity that is carried out is always through a process with the ceremony accompanying the various upakara ; ( 3 the culprit is the elect ( chosen girls who have not experienced puberty and is seen as a dancer who kesenengin chosen and blessed by God ; ( 4 where the play is holy places associated with yadnya ceremony at the local and pretended to be done every 210 days ( every six months in Bali or six times thirty- five days ; ( 5 the play time is the time it was considered kramat (sacred time and associated with yadnyadan ceremony ( 6 community supporters ( who believes that dance is a dance sacred Andir kesakralannya sourced from sungsungan Rangda .

  12. Representation of Gender Ideology in Indonesia Novels: A Study of The Reformation Era Novel

    Directory of Open Access Journals (Sweden)

    Yulianeta Yulianeta

    2016-05-01

    Full Text Available This research was based on a phenomenon that gender ideology practiced by a society might be reflected in the production of literary work. Thus, even though a novel is known as an imaginative work, its content and gender ideology could not be detached from social reality. The aims of this research were describing the role and gender identity, the types of gender ideology, and the gender relationship issues in the Indonesia novel written during reformation era. Gramsci’s theory of hegemony and gender perspective helped to describe the problems presented in this article. The formal object of this research was elaborating gender ideology presented in four novels written by Indonesian authors during reformation era, namely Saman by Ayu Utami, GeniJora by Abidah El Khalieqy, Nayla by Djenar Maesa Ayu, and Tanah Tabu by Anindita S. Tayf. The research method implemented was library research. This research showed the variety of ideologies that occupy literature as the site of struggle among ideologies. The results of the research are the four novels represent the patriarchal ideology, familialism ideology, ibuism ideology, and general gender ideology. The four gender ideologies create domestication of the position and the role of women. The existence and the organization of the gender ideology are supported by masculine hegemony in Indonesian culture.

  13. Modulation of the Absorption Maximum of Rhodopsin by Amino Acids in the C-terminus†

    Science.gov (United States)

    Yokoyama, Shozo; Tada, Takashi; Yamato, Takahisa

    2008-01-01

    Vision begins when light is absorbed by visual pigments. It is commonly believed that the absorption spectra of visual pigments are modulated by interactions between the retinal and amino acids within or near 4.5 Å of the retinal in the transmembrane (TM) segments. However, this dogma has not been rigorously tested. In this study, we show that the retinal-opsin interactions extend well beyond the retinal binding pocket. We found that, although it is positioned outside of TM segments, the C-terminus of the rhodopsin in the rockfish longspine thornyhead (Sebastolobus altivelis) modulates its λmax by interacting mainly with the last TM segment. Our results illustrate how amino acids in the C-terminus are likely to interact with the retinal. We anticipate our analyses to be a starting point for viewing the spectral tuning of visual pigments as interactions between the retinal and key amino acids that are distributed throughout the entire pigment. PMID:16922606

  14. Thermophysical Properties of Liquid AlTi-Based Alloys

    Science.gov (United States)

    Egry, I.; Holland-Moritz, D.; Novakovic, R.; Ricci, E.; Wunderlich, R.; Sobczak, N.

    2010-05-01

    The surface tension and density of three liquid AlTi-based alloys (AlTiV, AlTiNb, and AlTiTa) have been measured using electromagnetic levitation as a tool for containerless processing. Surface tension has been determined by the oscillating-drop method, while the density was measured using a shadowgraph technique. Both quantities were determined over a wide temperature range, including the undercooled regime. In addition, sessile-drop and pendant-drop experiments to determine the surface tension were performed in a recently built high-temperature furnace. The measured data were compared to thermodynamic calculations using phenomenological models and the Butler equation. Generally, good agreement was found.

  15. MISCELLANEOUS

    Institute of Scientific and Technical Information of China (English)

    1993-01-01

    930610 The retrospective study on the mortalityof 0-4 years old children in five monitoring vil-lages of Baoying County,Jiangsu Province.JI Ji-ayu(季家鈺).Women & Children Health Center,Baoying County,Baoying,225800.Chin RuralHealth Serv Administr 1993;13(3):44-45.According to the monitoring plan laid down bythe Department of Women and Children Affairs,Ministry of Public Health,a basal survey on theacute respiratory infection of the children in thewhole county was engaged.The condition of theretrospective study of the mortality of 0-4 yearsold children in 1990 of 5 monitoring villages(Sishui,Zhangshidang,Wangzhi,Chengjiao andXiaji)was reported and analyzed.There were2227 neonatal live births in 1990.The mortalityof the infants in the 5 monitoring villages was 93

  16. FENOMENA SEKS DALAM NOVEL INDONESIA MUTAKHIR KARYA PENGARANG PEREMPUAN: KAJIAN KRITIK SASTRA FEMINIS

    Directory of Open Access Journals (Sweden)

    Wiyatmi

    2006-10-01

    Full Text Available This research is aimed at describing and understanding (1 homo­sexuality phenomena in the current Indonesian novels, (2 the relationship between homosexuality phenomena with the existence of homosexual community in Indonesia, and(3 the relationship between homosexuality phenomena with feminist school. To achieve these aims, six novels are analyzed. They are (1 Larung by Ayu Utami, (2 Supernova by Dee (Dewi Lestari, (3 Mahadewi Mahadewi (4 Garis Tepi Seorang Lesbian by Herlinatien, (5 Tabularasa by Ratih Kumala, and (6 Dadaisme by Dewi Sartika. The result of the research shows that (1 homosexuality phenomena in the current Indonesian novels are reflected in theme and character problems, (2 these phenomena are related to the existence of homosexual community in Indonesia, whose members already demand that their existence be recognized in society, and (3 these phenomena are related to feminism, especially radical feminism that influences the authors’ creativity.

  17. Experience of Soviet Medicine in a Great Patriotic War, 1941-1945. Volume 16, Section 4.

    Science.gov (United States)

    1980-10-22

    admissible only during the unconditional readings. DOC = 80093637 PAGE Tabla 319. Duration of the hospital treatment of those amputated during Great... Tabla 323. Fraqusncy of the combination of the associated issues with the basic issues in injured people with the bullet break of shoulder (in the...Asrop Bonlia a -T-o (,) 2) rI ~ ~ a z- M H. AxN-rnH BoCHnLle 2QCicTPUVR U.H )Iea Xim., xIi -U’o2e 7,7 " 3,5 12,2 3,1 3.- 5%I. H. AYuH 6011 V .o3epa Xd- .cag

  18. NOVEL MLANTJARAN KA SASAK: FROM DILLEMATIC TEACHERS TO HUMOROUS ONES

    Directory of Open Access Journals (Sweden)

    I Nyoman Tingkat

    2015-07-01

    Full Text Available This article discusses the representation of the image of teachers in the novel entitled Mlantjaran ka Sasak written by Gde Srawana. Using the theory of literary sociology and the theory of representation as the basis of analysis, it was found that teachers were  imaged of being dilemmatic, humanistic idealistic, caring about the Balinese culture, and being humorous. Sociologically, the novel entitled Mlantjaran ka Sasak pictured the life background of the Balinese society during 1930s, indicated by the critical attitude of the intellectuals through I Made Sarati and Ida Ayu Priya, the characters who played a role as teachers, towards the caste feudalism in marriage. Such a critical attitude was expressed in such an inciting way that the ethic and aesthetic values needed to educate society were not neglected.

  19. The Forgotten Great War: Viewpoint of a Political Scientist. Book review: Russia in the Great War of 1914-1918. The images and texts / ed. by A. K. Sorokin, A. Yu. Shutov ; auth.-comp. K. M. Anderson, et al. – Moscow : Politicheskaya entsik

    Directory of Open Access Journals (Sweden)

    Pankratov Sergey Anatolyevich

    2015-04-01

    Full Text Available This publication is a review of the book Russia in the Great War of 1914-1918. The images and texts, prepared by the team of authors and edited by A.K. Sorokin and A.Yu. Shutov in the context of joint activity of the Russian Society of Political Scientists,Faculty of Political Science of Lomonosov Moscow State University, Russian State Archive of Social and Political History. The reviewer characterizes the specifics of the publication which represents an attempt to reveal the atmosphere of the century-old events, semantic plots, images of people and their actions through the photographs from the pages of periodicals, posters and cartoons, leaflets and official documents of the First World War.

  20. Kajian Aspek Ketidaksadaran dalam Karya Seni Rupa Indonesia Periode 2000-2011

    Directory of Open Access Journals (Sweden)

    Irma Damajanti

    2014-11-01

    Full Text Available Pada era seni rupa kontemporer, pembacaan karya tidak lagi hanya terbatas padahasil analisis dan interpretasi unsur-unsur formal, namun juga mempertimbangkanketerkaitannya dengan psikobiografi seniman sehingga dapat memberikan gambaranyang lebih lengkap tentang karakter seni rupa yang multifaset. Penelitian inidirancang untuk mengidentifikasi representasi aspek ketidaksadaran dalam karya senirupa Indonesia dan menginterpretasikan simbol-simbolnya. Seniman yang karyanyadipilih sebagai studi kasus dalam penelitian ini adalah I Gusti Ayu Kadek Murniasih,Entang Wiharso, dan Ugo Untoro. Pendekatan yang digunakan adalah pendekatanmultidisiplin (psikologi seni dan semiotika. Berdasarkan hasil analisis, aspekketidaksadaran yang direpresentasikan melalui simbol-simbol visual yang khas dalamkarya ketiga seniman cenderung lahir dari pengalaman traumatik yang melahirkanketakutan, kesakitan, dan agresi. Melukis bagi ketiganya adalah proses katarsis,media untuk mensublimasikan/memperhalus dorongan-dorongan naluri ketakutan,kesakitan, dan agresi ke dalam bentuk karya yang dapat diterima dan diapresiasidengan baik oleh masyarakat. A Study on Unconsciousness Aspect in the 2000-2011 Works of Indonesia VisualArt. In the era of contemporary visual art, the works of art cannot be read merelylimited to the analysis and interpretation of the formal elements but have to include theconsideration about the artist’s psychobiography so that there will be a more completepicture about the multifaceted visual arts. This research is carried out to identify therepresentation of the unconsciousness aspect in the works of visual arts and then interpretthe symbols. The selected samples are the works of I Gusti Ayu Kadek Murniasih,Entang Wiharso, and Ugo Untoro. The multidisciplinary (art psychology and semioticsapproach is applied within the qualitative method used. Based on the analysis result,the unconsciousness aspect represented in unique visual symbols in the works of

  1. PRODUKSI PROTEIN REKOMBINAN HORMON PERTUMBUHAN IKAN KERAPU

    Directory of Open Access Journals (Sweden)

    Irvan Faizal

    2012-08-01

    Full Text Available Salah satu spesies ikan yang menjadi target produksi perikanan budidaya nasional adalah ikan kerapu tikus (Cromileptes altivelis. Ikan kerapu tikus merupakan ikan laut budidaya komoditas ekspor, namun laju pertumbuhannya sangat lambat. Oleh karena itu, perlu dilakukan usaha budidaya yang mampu menaikkan laju pertumbuhan ikan kerapu tikus. Pendekatan nutrisi melalui penggunaan hormon pertumbuhan (Growth Hormone, GH pada usaha budidaya diyakini mampu meningkatkan kecepatan tumbuh ikan budidaya. Pada penelitian ini, melalui teknologi DNA rekombinan telah dilakukan isolasi dan identifikasi gen GH yang selanjutnya dilakukan produksi protein rekombinan GH (recombinant Growth Hormone, rGH dengan memanfaatkan penggunaan bakteri E. coli. Dalam pelaksanaan penelitian dilakukan konstruksi rGH yang menghasilkan bakteri E. coli BL21 (DE3 yang mampu memproduksi protein rGH. Produksi rGH dilakukan pada skala bioreaktor. Proses isolasi produk rGH-nya dalam bentuk pellet inclusion bodies yang selanjutnya dicampur dengan pelet pakan komersil hingga konsentrasi akhir protein dalam pakan mencapai 1 ng/6 mg pakan, di mana setelah dikering-anginkan, pelet pakan protein rekombinan GH dapat diaplikasikan untuk budidaya ikan kerapu tikus dan ikan budidaya lainnya.

  2. ENERGY OF NOVELS SAMAN, NAYLA, AND PETIR IN LITERARY PUBLISHING INDUSTRY

    Directory of Open Access Journals (Sweden)

    Sugiarti -

    2012-11-01

    Full Text Available The research was based on the notion that novels Saman, Nayla, and Petir haveuniqueness in the exploration of ideas, narration style, and such typical techniques that creatednew esthetics. Besides, the literary works were written by three creative, innovative andknowledgeable young women who, in expressing their ideas and thoughts, were able to totallyframe story and give energy to the novels Saman, Nayla, and Petir. In the history of Indonesiancontemporary literary works, they (Ayu Utami, Djenar Maesa Ayu, and Dewi Lestari wereknown to have produce monumental works, which in turn received good responses in Indonesianliterature publishing industry.This research is aimed at describing (a thematic aspect and energy of the novels Saman,Nayla and Petir, which encourages literature publishing industry to publish; (b readers’reception of the themes novels Saman, Nayla, and Petir in social and cultural change andpublishing industry; (c relevance of cultural industry and economic praxis of readers, bookmarket and publishing industry of literary history. The research method used was descriptivewith qualitative and hermeneutic approaches. Meanwhile, the data was deeply analyzed in theunderstanding, content, and context. The analysis was conducted in cyclic and counter balancedways to gain deep understanding of writers’ thoughts, readers’ responses to the literary works,and the role of publishing industry with available market prospect. The research was based ontheory of post structuralism, reception, intertext, and cultural industry in literature.The research results showed that (1 thematic aspects and energy of the novels Saman,Nayla, and Petir as a context described women’s concern about social and cultural structure,patriarchy culture, and conflicts between traditional and modern structures. There were vulgarand jumping expressions, contrasting dictions, fantasy and symbolic language, the use of naturalstyle, free narrative, surprising and

  3. A CRITICAL REVIEW ON AYURVEDIC CONCEPT OF AGNIMANDYA (LOSS OF APPETITE

    Directory of Open Access Journals (Sweden)

    Singh Akhilesh Kumar

    2012-04-01

    Full Text Available Human beings, in order to adjust themselves in the modest era, have been compelled to become fast and mechanical. This is the reason why they can’t give proper attention to daily and seasonal regimes, exercise and diet. This change in lifestyle is responsible for replacement of Shad-rasatmak Ahara by preserved and fast foods, bakery products, shift duties (i.e.Ratree jagran, stress. All these factors making them more and more susceptible for diseases generated by Agnimandya. According to them Mandagni is root cause of every disease. Due to this Mandagni Ahara-Pachan Kriya gets affected leading to production of Sama Ahara-Rasa which ultimately causes the Rasa Dhatwagni Mandya and Uttarottar Dhatu remains malnourished. Agni plays the key role in the process of bio-transformation. This is the reason why Ayurvedic Samhitas has given a lot of importance to Agni. The Ayu, Varna, Bala, Swasthya, Utsah, Buddhi, Kanti, Oja, Teja and Prana of human beings mainly depend on the status of Agni, because of this Agni should be kept in its Prakut state.

  4. UPAYA PENINGKATAN PENGETAHUAN TENTANG OBAT TRADISIONAL DAN PEMANFAATANNYA MELALUI PELATIHAN PADA USILA

    Directory of Open Access Journals (Sweden)

    Herti Maryani

    2012-11-01

    Full Text Available By The Older People Integrated Health Post under the supervision of health centers, the traditional medicines from medicinal plants could be taught to older people to have benefit for self medication. The use of herbal medicine is prioritized to treat diseases that often occur among older people. This study aimed to enhance the knowledge and use of herbal plants as traditional medicines for older people. Data were collected by training with modul, practice, and talkative. The training were done on June 2007 in Surabaya. The respondents were 29 older persons from Kelurahan Medokan Ayu and Kelurahan South Krembangan, Surabaya city. Pre and post test were done on the knowledge. and practice of use of herbal medicine.The post test on knowledge was done soon after the training, meanwhile the post on practice were done three months after the training. Results showed there was an improvement of knowledge after the training for herbal medicine identification (a= 0.00, advantages of herbal medicine, to treat diseases and how to make traditional medicines (a= 0.04. Moreover, after training the respondents who take advantage of traditional medicinese and variety of herbal medicine are raised. Key words: older people, herbal medicine, training

  5. New Publications and Videos

    Directory of Open Access Journals (Sweden)

    Igor V. Karyakin

    2017-01-01

    Full Text Available Karyakin I.V. Eagles of Russia and Kazakhstan: ranges and electric-grid danger zones. Novosibirsk: Sibecocentr, 2016. 36 pp. ISBN 978-5-9908314-0-7. Forsman D. Flight Identification of Raptors of Europe, North Africa and the Middle East. London: Bloomsbury Publishing, 2016: 1–544. ISBN-13: 9781472913616 Birds of Prey in the Northern Eurasia: Problems and adaptations in current environment: Proceedings of the VII International Conference on Birds of Prey and Owls of Northern Eurasia, Sochi, 19-24 September 2016 / Ed. V.P. Belik. Rostov-on-Don, 2016. 614 pp. ISBN 978-5-9275-2047-3 The Harriers of the Palearctic: Systematics, distribution and features of ecology in Northern Eurasia: Proceedings of the VII International Conference on Birds of Prey and Owls of Northern Eurasia, Sochi, 19-24 September 2016 / Eds. I.V. Fefelov, A.Yu. Sokolov. Rostov-on-Don, 2016. 180 p. ISBN 978-5-9275-2053-4

  6. Development of a digital TV receivers test suite

    Directory of Open Access Journals (Sweden)

    Pablo Flores-Guridi

    2015-01-01

    Full Text Available El despliegue de la televisión digital en Uruguay está en curso , siguiendo el estándar ISDB-Tb. Ya se completó el proceso de a signación de canales y, después de aprobado un protocolo de homologación, se ha comenzado a importar receptores o decodificadores desde principios de 2014. La certific ación es de capital importancia para acelerar la migra ción y para brindar una plataforma a los desarrolladores locales. El presente artículo resume los aspectos clave de este trabajo: el análisis de las norm as, la elaboración del Protoco lo de Homologación y la experiencia obtenida en la aplicación de las pruebas. Su objetivo es compartir estas experienc ias para contr ibuir en los procesos de adopción de la TV di gital, con lo que se espera ayu dar a que otros países o actore s puedan evitar problemas de com patibilidad o cumplimiento de las normas que aparecieron durante este traba jo, tanto en la regulación como e n los dispositivos ensayados.

  7. Brain Enhancing Ingredients from Āyurvedic Medicine: Quintessential Example of Bacopa monniera, a Narrative Review

    Directory of Open Access Journals (Sweden)

    Hemant K. Singh

    2013-02-01

    Full Text Available Āyurveda, the science (ved of life (ayu, owing its origin to Veda, the oldest recorded wisdom of human civilization written in 3500 BCE, contains extensive knowledge of various diseases and their therapeutic approaches. It essentially relied on nature and the immune system of an individual, and therapeutic interventions were introduced only to augment the immune system. Āyurveda had eight specialties, including psycho-neuroscience (a combination of psychology, clinical psychology and psychiatry and a unique promotive therapy encompassing nutrition, rejuvenation and geriatrics. The symptoms of various brain disorders, including memory disorder, were well defined. The goal of Āyurveda was to help an individual to achieve his cherished goal of leading a healthy life of 100 years. To achieve this, great emphasis was laid on nutrition, diet and a good conduct by the two great exponents of Āyurveda viz. Carak and Suśruta. By following these regimens, an individual could lead a less stressful life free from emotional disturbances. Both Carak and Suśruta had believed that these in combination with rasayana (rejuvenating plants could enable an individual to lead a healthy life of 100 years.

  8. WACANA KEKERASAN DAN RESISTENSI PEREMPUAN DALAM FILM KARYA SUTRADARA PEREMPUAN

    Directory of Open Access Journals (Sweden)

    Liestianingsih Dwi Dayanti

    2011-08-01

    Full Text Available Mereka Bilang Saya Monyet, a movie by woman director Djenar Maesa Ayu, was a film about women with a different perspective. This movie reveals a discourse on both violence and women’s resistance against it. The dimensions shown included psychological, physical, and sexual violence between parents and their child, an adult men with his daughter, and adult men with adult women. Violence appears because of the unbalanced relationship between subject and object. The effects of this violence can take the form of fear, anger, or a feeling of inferiority and unworthiness, and the victim feels encouraged to act on it. Discourse related to gender-based violence is a result of a patriarchal cultural construction. In the film, resistance was portrayed through the victim’s suffering, anger, and physical resistance. Women directors perceived these issues from a different perspective. It is not enough to understand violence against women only from the normative perspective of law and social obligation. This issue must be understood by positioning women as the subject (survivor and recognizing that violence against women is a crime against humanity.

  9. Spilled Oils: Static Mixtures or Dynamic Weathering and Bioavailability?

    Directory of Open Access Journals (Sweden)

    Mark G Carls

    Full Text Available Polynuclear aromatic hydrocarbons (PAHs from sequestered MV Selendang Ayu oil were biologically available in 2008, 3.6 y after it was spilled along Unalaska Island, Alaska. Thermodynamically driven weathering was the most probable mechanism of organism exposure to PAHs. Alkane and PAH composition in oil changed over time as smaller constituents were preferentially lost, indicative of weathering. In contrast, composition of the largest compounds (biomarkers including triterpanes, hopanes, and steranes remained unchanged. Smaller molecules (the PAHs lost from stranded oil were observed in indigenous mussels and passive samplers deployed in July 2008. Concentration and composition of PAHs were significantly different than in a non-oiled reference area and patterns observed in mussels were repeated in passive samplers deployed in three zones (intertidal, subtidal, and water. Thus, hydrocarbons lost from one compartment (sequestered whole oil were detectable in another (mussels and passive samplers implying aqueous transfer. Quantities of mobile oil constituents were small, yielding uptake concentrations that are likely inconsequential for mussels, but the sensitivity provided by bioaccumulation and passive sampler uptake ensured that dissolved hydrocarbons were detectable.

  10. Mercury and selenium content of Taiwanese seafood.

    Science.gov (United States)

    Fang, G C; Nam, D H; Basu, N

    2011-01-01

    Fish consumption is avid in Taiwan (and other Asian nations), but little is known about the mercury and selenium content in local seafood. This paper reports on total mercury, methylmercury and selenium levels from 14 commonly consumed seafood items obtained from Taichung, Taiwan. Mean total mercury concentrations varied nearly 100-fold across species. Fifty per cent of the marlins sampled and 35% of the sharks exceeded the 0.3 µg g(-1) US Environmental Protection Agency (USEPA) guideline. Methylmercury comprised a majority of the total mercury in all species. In all species studied there was a molar excess of selenium over mercury. The rank order of mean selenium-mercury molar ratios was red tilapia (166.8) > abura (87.9) > river prawn (82.4) > whiteleg shrimp (64.2) > butterfish (44.6) > milkfish (37.0) > tuna (15.6) > grouper (13.9) > ayu (13.4) > coral hind (13.0) > weever (11.8) > saury (9.0) > shark (7.8) > marlin (4.2).

  11. The English Borrowings and the Indonesian-English Codeswitching in Two Collections of Blog Short-Stories

    Directory of Open Access Journals (Sweden)

    Anna Marietta da Silva

    2013-01-01

    Full Text Available If one has ever read fiction works by prominent Indonesian authors from 1920‟s Abdul Muis, and Marah Rusli, 1930‟s Sutan Takdir Alisjahbana and Armijn Pane, 1945‟s Chairil Anwar and Achdiat K. Mihardja, 1950-1960‟s Pramoedya Ananta Toer, W. S. Rendra, and N. H. Dini, 1960-1970‟s Taufik Ismail, Goenawan Moehammad, and Sapardi Djoko Darmono, and compared their work with those of the 1990‟s Hilman Sriwijaya and Ayu Utami, and the 2000‟s Hirata, one must have found many differences among the works. More obvious distinction will be discovered if all of the works previously mentioned are contrasted with those of 2000‟s teen writers like Arunita, Mentari, Andries, and recently Dika, especially in terms of style and vocabulary. The most recent teen fictions have employed much colloquial Indonesian or Malay-Betawi dialect (Djenar, 2008, and English lexical items. This study shows that in Dika‟s blog short-stories alone, English borrowings and Indonesian-English code-switching were commonly found. English seems to be the third language after the colloquial and standard Indonesian with which the author thinks

  12. MORPHO-CHEMICAL DESCRIPTION AND ANTIMICROBIAL ACTIVITY OF DIFFERENT OCIMUM SPECIES

    Directory of Open Access Journals (Sweden)

    KAKARAPARTHI PANDU SASTRY

    2012-12-01

    Full Text Available Basil is a popular medicinal and culinary herb, and its essential oils have been used extensively for many years in food products, perfumery, dental and oral products. Basil essential oils and their principal constituents were found to exhibit antimicrobial activity against a wide range of Gram-negative and Gram-positive bacteria, yeast, and mould. The essential oils obtained from aerial parts of three different species of Ocimum comprising twenty one germplasm lines were investigated for their essential oil composition and antimicrobial activity during 2010. Essential oils from seventeen germplasm lines in Ocimum basilicum and two each in Ocimum tenuiflorum and Ocimum gratissimum were investigated for anti-microbial activity against four bacterial strains (Staphylococcus aureus, Bacillus sps., Escherichia coli and Pseudomonas aeruginosa. The morpho-chemotypes exhibited wide variability for morphological and chemical traits. Anti-bacterial activity was found to be high for Staphylococcus aureus, moderate for Escherichia coli, low for Bacillus and Pseudomonas aeruginosa was highly resistant. The essential oils of Pale Green-Broad Leaves (O. basilicum and CIM Ayu (O. gratissimum exhibited significant antibacterial activity against both S. aureus and E. coli signifying them promising for anti-bacterial activity. No relationship was observed between chemotype specificity and anti-bacterial activity, indicating that apart from major components of essential oil, minor components and other factors may be responsible for anti-microbial activities.

  13. Age determination and validation studies of marine fishes: do deep-dwellers live longer?

    Science.gov (United States)

    Cailliet, G M; Andrews, A H; Burton, E J; Watters, D L; Kline, D E; Ferry-Graham, L A

    2001-04-01

    Age determination and validation studies on deep-water marine fishes indicate they are difficult to age and often long-lived. Techniques for the determination of age in individual fish includes growth-zone analysis of vertebral centra, fin rays and spines, other skeletal structures, and otoliths (there are three sets of otoliths in most bony fish semicircular canals, each of which is made of calcium carbonate). Most have regular increments deposited as the fish (and its semicircular canals) grows. The most commonly used otolith for age determination is the largest one called the sagitta. Age validation techniques include: (1) tag-recapture, often combined with oxytetracycline injection and analysis in growth-zones of bone upon recapture; (2) analysis of growth-zones over time; and (3) radiometric approaches utilizing a known radioactive decay series as an independent chronometer in otoliths from bony fishes. We briefly summarize previous studies using these three validation approaches and present results from several of our radiometric studies on deep-water, bony fishes recently subjected to expanding fisheries. Radiometric age validation results are presented for four species of scorpaenid fishes (the bank, Sebastes rufus, and bocaccio, S. paucispinis, rockfishes, and two thornyhead species, Sebastolobus altivelis and S. alascanus). In addition, our analysis of scorpaenids indicates that longevity increases exponentially with maximum depth of occurrence. The reason that the deep-water forms of scorpaenid fishes are long-lived is uncertain. Their longevity, however, may be related to altered physiological processes relative to environmental parameters like low temperature, high pressures, low light levels, low oxygen, and poor food resources.

  14. Pemberian Fikosianin Spirulina Meningkatkan Jumlah Sel Darah, Aktivitas Fagositosis, dan Pertumbuhan Ikan Kerapu Bebek Juvenil (ADMINISTRATION OF SPIRULINA PHYCOCYANIN ENHANCES BLOOD CELLS, PHAGOCYTIC ACTIVITY AND GROWTH IN HUMPBACK GROUPER JUVENILE

    Directory of Open Access Journals (Sweden)

    Woro Hastuti Satyantini

    2014-05-01

    Full Text Available The aim of this study was to investigate effects of Spirulina phycocyanin on the total  blood cell count,phagocytic activity, and growth of humpback grouper fish, Cromileptes altivelis juvenil.  Fishes were fedwith a diet containing   0, 150, 250, 350 dan 450 mg  phycocyanin per kg diet for four weeks and eachtreatment was triplicates.  Initial body weight  of  grouper was  8.46 ± 0.22 g with a density of 10 fish per56 litre volume. The total count of  erythrocytes and leucocytes increased until the fourth week of rearingperiod. The highest of total erythrocyte and leucocytes were observed in fish treated with 150 mg phycocyaninper kg diet ( 13.17 x  105 cells/mm3 and 8.93 x 105 cells/mm3 respectively which were not significantlydifferent (P>0.05 to those treated with 250 mg phycocyanin per kg diet. The total leucocytes and phagocyticactivity of fish fed diet containing  250 mg phycocyanin  per kg diet (8.49 x 105 cells/mm3 and 59.67%respectively were significantly higher  (P <0.05 to those of control group. The highest of final weight(Wt=14.32 g and weight growth (G=5.89g and lowest of feed conversion ratio (FCR=1.13 were obtainedin fish treated with  250 mg phycocyanin per kg diet which were  significantly  higher  (P <0.05 than thosefed control diet. The data showed that  the addition of  phycocyanin 250 mg/kg diet enhances the totalleukocyte count, phagocytic activity and the growth of humpback grouper juvenil.

  15. Kunst, Kosmos, Kaste. Weibliche Körperinszenierungen, Tanz und Aspekte der Bewahrung balinesischer Kultur in Oka Rusminis Tarian Bumi [Art, Cosmos, Caste. Female Stagings of the Body, Dance and Aspects of Preserving Balinese Culture in Oka Rusmini‘s Tarian Bumi

    Directory of Open Access Journals (Sweden)

    Monika Arnez

    2008-01-01

    Full Text Available Immer mehr indonesische Autorinnen haben insbesondere seit dem Sturz Präsident Suhartos die literarische Bühne erobert. Viele ihrer Bücher wurden Bestseller, mit bis zu 100.000 verkauften Kopien. Diese zunehmende Popularität hängt vor allem mit Tendenzen der Liberalisierung, Aufhebung der Zensur und einer Redefinition der Rolle der Frau zusammen. Einige Schriftstellerinnen machten in der letzten Dekade durch die offene Thematisierung von Sexualität und Begehren der Frau auf sich aufmerksam, ein Tabuthema, das während der Neuen Ordnung nicht in dieser Weise hätte diskutiert werden können. Die meisten dieser Autorinnen, deren Literatur in den Medien oftmals mit dem umstrittenen Begriff sastrawangi (Duftliteratur bezeichnet wird, wählen als Handlungsschauplätze ihrer Erzählungen urbane Zentren Javas. Dieses Paper diskutiert den Roman Tarian Bumi (Erdentanz, 2000 von Oka Rusmini, die den Diskurs um den weiblichen Körper und Sexualität de facto noch vor der ‚Pionierin’ der sogenannten sastrawangi eröffnet hat, Ayu Utami. Das Paper möchte einen Beitrag dazu leisten, die Verflechtungen von weiblichen Körperinszenierungen, Tanz und Aspekte der Bewahrung der balinesischen Kultur aufzuzeigen. Ein besonderes Augenmerk liegt auf der Frage, wie die Körperinszenierungen genutzt werden, um die Rolle der Frau in der balinesischen Gesellschaft innerhalb des Spannungsfeldes des hinduistischen Kastensystems, der Hierarchie und vor dem Hintergrund der Problematik von Tourismus, postkolonialer Kritik und Bewahrung balinesischer Kultur im Wandel der Zeit zu charakterisieren.An increasing number of Indonesian women authors have entered the literary stage since the fall of Suharto. Many of their books have become bestsellers, selling up to 100.000 copies. Reasons for this rising popularity of literature by women authors are tendencies of liberalization, lifting of censorship and a redefinition of women’s role. Some female authors have attracted

  16. El clima de aula según becarios 18 y alumnos regulares de una universidad privada de Lima

    Directory of Open Access Journals (Sweden)

    Julio B. Anaya

    2015-03-01

    Full Text Available La inserción de beneficiarios pobres del programa social Beca 18 en universidades limeñas es un fenómeno reciente que amerita estudios relativos a su adaptación y desempeño académico. En la presente investigación se analizaron las respuestas de becarios 18 en USIL a un cuestionario de percepciones del clima de aula, y se les comparó con las de alumnos regulares para establecer si había diferencias. Ambos participaban en el Curso de Análisis Matemático que se imparte a estudiantes de ingeniería. En el contexto general de climas de aula bastante positivos, los becarios se diferenciaron ligeramente de los alumnos regulares por una percepción más positiva del comportamiento del profesor en términos del ambiente de trabajo creado, el ritmo de la clase, el interés por el estudiante y el fomento de la cooperación y trabajo grupal. Mayor diferencia se observó respecto a la evitación de un clima de competición; esto debería investigarse a mayor profundidad. De otro lado, los becarios se quejaron más de la falta de ayu da por parte de otros alumnos, lo cual se explica por la mayor solidaridad existente en provincias, cuya población es más colectivista que la de Lima.

  17. A review of the genus Sclerocollum Schmidt & Paperna, 1978 (Acanthocephala: Cavisomidae) from rabbitfishes (Siganidae) in the Indian and Pacific Oceans.

    Science.gov (United States)

    Pichelin, Sylvie; Smales, Lesley R; Cribb, Thomas Herbert

    2016-02-01

    Seven of the eleven species of Siganus Richardson (Siganidae) collected off the coasts of Australia, New Caledonia, French Polynesia and Palau were infected with species of Sclerocollum Schmidt & Paperna, 1978 (Acanthocephala: Cavisomidae). A Principal Component Analysis (PCA) and a Discriminant Analysis were performed on a morphometric dataset of specimens of Sclerocollum including borrowed type-specimens of Sc. rubrimaris Schmidt & Paperna, 1978 from the Indian Ocean and of Sc. robustum Edmonds, 1964, the only acanthocephalan species known previously from an Australian siganid. These analyses showed that the lengths of proboscis hooks were useful variables for separating specimens into groups and supported the presence of two known species (Sc. robustum and Sc. rubrimaris) and one new species (Sc. australis n. sp.) in Australian waters. We found Sc. robustum in Siganus lineatus (Valenciennes) from off Queensland and Sc. rubrimaris in S. fuscescens (Houttuyn) from off Western Australia and Queensland, S. punctatissimus Fowler & Bean from off Queensland and S. argenteus (Quoy & Gaimard), S. corallinus (Valenciennes), S. canaliculatus (Park) and S. doliatus Guérin-Méneville from off New Caledonia (all new host and locality records) which we compared with museum specimens of Sc. rubrimaris from S. rivulatus Forsskål & Niebuhr and S. argenteus [as S. rostratus (Valenciennes)] from the Red Sea. The third species, Sclerocollum australis n. sp., was found only in S. corallinus and S. doliatus from off Queensland. Sclerocollum australis n. sp. can be distinguished from its congeners by a unique combination of characters of the proboscis armature, including lengths of hooks 1-7. Specimens of Sclerocollum were also found in Zebrasoma velifer (Bloch) (Acanthuridae) from off Queensland, and Coradion altivelis McCulloch (Chaetodontidae) and Heniochus acuminatus (Linnaeus) (Chaetodontidae) from off New Caledonia. No acanthocephalans were found in siganids collected from

  18. Ground-based simulation of the Earth's upper atmosphere oxygen impact on polymer composites with nanosized fillers

    Science.gov (United States)

    Novikov, Lev; Chernik, Vladimir; Voronina, Ekaterina; Chechenin, Nikolay; Samokhina, Maria S.; Bondarenko, Gennady G.; Gaidar, Anna I.; Vorobyeva, Ekaterina A.; Petrov, Dmitrii V.; Chirskaya, Natalia P.

    under AO impact [2,4]. REFERENCES 1. K.B. Vernigorov, A.Yu. Alent'ev, A.M. Muzafarov, L.S. Novikov, V.N. Chernik. J. Surf. Ingestig.-X-Ray Synchro. 5, 263 (2011). 2. E.N. Voronina, L.S. Novikov, V.N. Chernik, et al. Inorg. Mat.: Appl. Res. 3, 95 (2012). 3. N.G. Chechenin, P.N. Chernykh, E.A. Vorobyeva, O.S. Timofeev. Appl. Surf. Science, 275, 217-221 (2013). 4. E.N. Voronina, L.S. Novikov. RSC Adv., 2013, 3 (35), 15362.

  19. Molecular characterization and functional analysis of TRAF6 in orange-spotted grouper (Epinephelus coioides).

    Science.gov (United States)

    Li, Yan-Wei; Li, Xia; Xiao, Xi-Xi; Zhao, Fei; Luo, Xiao-Chun; Dan, Xue-Ming; Li, An-Xing

    2014-05-01

    Tumor necrosis factor receptor (TNFR)-associated factor 6 (TRAF6) is a crucial signal transducer in both the TNFR superfamily and Toll-like receptor/interleukin 1R family. Although significant progress has been made in clarifying the role of TRAF6 in mammals, the function of TRAF6 in fish is still poorly understood. In this study, we cloned the orange-spotted grouper (Epinephelus coioides) TRAF6 (EcTRAF6) cDNA, with an open reading frame of 1713bp encoding 570 amino acids. Sequence analysis indicated that EcTRAF6 contains the four characteristic domains conserved in the TRAF family, including an N-terminal RING finger, two zinc fingers, a coiled-coil domain, and a C-terminal MATH domain. Homology alignment and phylogenetic analysis demonstrated that EcTRAF6 shares high sequence identity with TRAF6 of other fish species. The EcTRAF6 gene contains seven exons and six introns, which is similar to the organization in ayu, but not in the common carp, human, or mouse (six exons and five introns). EcTRAF6 transcripts were broadly expressed in all tissues tested, and increased after infection with Cryptocaryon irritans. Intracellular localization showed EcTRAF6 was distributed mainly in the cytoplasm. Over-expression of wild type (WT) EcTRAF6, truncated forms of EcTRAF6, including ΔZinc finger 2 and ΔMATH, and a mutant of C78A activated NF-κB strongly in HEK293T cells; whereas truncations, including ΔRING, ΔZinc finger 1 and Δcoiled-coiled, and a mutant of K132R induced the activity of NF-κB slightly compared to WT EcTRAF6, implying the latter has a more crucial role in downstream signal transduction. Together, these results suggested EcTRAF6 functions like that of mammals to activate NF-κB, and it might have an important role in host defense against parasitic infections.

  20. Geodynamic environments of ultra-slow spreading

    Science.gov (United States)

    Kokhan, Andrey; Dubinin, Evgeny

    2015-04-01

    part of Bransfield rift); 2. During back-arc inter-subduction spreading (Ayu trough, northern Fiji basin), 3. During diffuse back-arc spreading (area on the south-eastern border of Scotia sea), 4. During back-arc spreading under splitting of island arc (northern extremity of Mariana trough). Each of the geodynamic environments is characterized by peculiar topographic, geological and geophysical features forming under the same spreading velocities. Development of ultra-slow spreading in each of these environments results in formation of peculiar extension sedimentary basins.

  1. Heterogeneous Data Fusion Methods for Disaster Risk Assessment using Grid Infrastructure

    Science.gov (United States)

    Kussul, Nataliia; Skakun, Sergii; Shelestov, Andrii

    2014-05-01

    index (VHI) derived from NOAA satellites, and the extreme value theory techniques. Drought vulnerability is assessed by estimating the crop areas and crop yield to quantify potential impact of a drought on crop production. Finally, drought hazard and vulnerability maps are fused to derive a drought risk map. [1] N.N. Kussul, B.V. Sokolov, Y.I. Zyelyk, V.A. Zelentsov, S.V. Skakun, and A.Yu. Shelestov, "Disaster Risk Assessment Based on Heterogeneous Geospatial Information," J. of Autom. and Inf. Sci., 42(12), pp. 32-45, 2010. [2] S. Skakun, N. Kussul, A. Shelestov, and O. Kussul, "Flood Hazard and Flood Risk Assessment Using a Time Series of Satellite Images: A Case Study in Namibia," Risk Analysis, 2013, doi: 10.1111/risa.12156. [3] L. Hluchy, N. Kussul, A. Shelestov, S. Skakun, O. Kravchenko, Y. Gripich, P. Kopp, E. Lupian, "The Data Fusion Grid Infrastructure: Project Objectives and Achievements," Computing and Informatics, vol. 29, no. 2, pp. 319-334, 2010.

  2. Outcaste by Choice: Re-Genderings in a Short Story by Oka Rusmini

    Directory of Open Access Journals (Sweden)

    Harry Aveling

    2010-11-01

    Full Text Available Ida Ayu Oka Rusmini is a major contemporary Indonesian author. She has published two novels, Tarian Bumi (2000 and Kenanga (2003a, a collection of short stories, (Sagra, 2001, and a volume of poetry, Patiwangi (2003b, republished in 2007 as Warna Kita, with the omission of some 12 poems. Born in Jakarta in 1967 of Balinese parents, she was a member of the highest Balinese caste, the brahmana caste, but renounced this status, including her title, after her marriage to the East Javanese essayist and poet Arif B. Prasetyo. Oka Rusmini is a graduate of the Indonesian Studies Department, Udayana University, and lives in Den Pasar where she works as a journalist for the Bali Post. Most of Oka Rusmini’s prose works explore the constraints into which the socioreligious practices of caste place all members of society, but most especially women. Both of her novels tell of a woman’s abandonment of her brahmin caste status as the result of her marriage to a sudra. The title of the poetry book, Patiwangi, refers to the ritual practice by which this degradation is confirmed, and the poem which gives the book its title bears the footnote: ‘Patiwangi: pati = death; wangi = fragrant. Patiwangi is a ritual that is performed on a noble women in her Village Temple to remove her noble status as a consequence of having married a man of a lower caste. The ritual often has a serious psychological impact on noble women’ (107. In both novels, and many short stories and poems, their loss of status brings enormous scorn and hardship to the major woman characters. Nevertheless, as we shall see, stepping outside patriarchally-dominated caste ties may also provide an ambiguous freedom for any woman who is positioned to take advantage of the opportunities which the modern, potentially secular, nation state of Indonesia, offers her. In this paper, I am interested in the way in which the short story, ‘Cenana’ (Sagra, 270-318, uses a traditional myth to deal

  3. Research the dynamical characteristics of slow deformation waves as a rock massif response to explosions during its outworking

    Science.gov (United States)

    Hachay, Olga; Khachay, Oleg; Shipeev, Oleg

    2015-04-01

    mine to estimate the changing state of the rock burst in the massif by its outworking. As a result we have selected a typical morphology of massif response phase trajectories, which were locally, over time, in a stable state: on the phase plane the local area presented as a ball of twisted trajectories with some not far removed points from the ball, which had not exceeded energy of more than 105 joules. For some time intervals those removed points exceeded 105 joules, achieving 106 joules and even 109 joules (Hachay et al., 2010). Introduction of the additional velocity parameter of slow deformation wave propagation allowed us, with the use of phase diagrams, to identify the hierarchic structure. Further, we can use that information for the modelling and interpretation of seismic and deformation waves in hierarchic structures (Hachay et al., 2012). That method can be useful in building-up an understanding of the resonance outshooting of catastrophic dynamic events and prevent these events. References 1.Chulichkov A. (2003) Mathematical models of nonlinear dynamics. Moscow: Phismatlit. 294p. 2.Hachay O., Khachay O.Yu., Klimko V., et al. (2010) Reflection of synergetic features of rock massif state under the man-caused influence from the data of a seismological catalogue. Mining Information-Analytic Bulletin, Moscow, Mining book, 6, pp.259-271. 3.Hachay O., Khachay A.Yu. (2012) Research of stress-deforming state of hierarchic medium. Proceedings of the Third Tectonics and Physics Conference at the Institute of the Physics of the Earth 8-12 October 2012, Moscow, IFZ RAS, pp.114-117. 4.Kurlenja M., Oparin V., Vostrikov V. (1993) About forming elastic wave trains by impulse excitation of block medium. Waves of pendulum type Uμ. DAN USSR, V.133, 4, pp.475-481. 5.Naimark Yu., Landa P. (2009). Stochastic and chaotic oscillations. Moscow, Knigniy dom ,'LIBROKOM', 424 p. 7.Oparin V., Vostrikov V., Tapsiev A. et al. (2006) About one kinematic criterion of forecasting of the

  4. EDITORIAL

    Directory of Open Access Journals (Sweden)

    Gusti Ayu Made Suartika

    2014-07-01

    architect. Dinar studies the stilt houses of Loloan community, Jembrana, Bali. She underlines that the form of stilt houses has changed over time in line with various elements including socio-cultural aspects, economic factors, building age, land availability, and occupants' preferences. The sixth article is written by I Nengah Riana, Widiastuti, and Ida Bagus Gde Primayatna, academic staff from the Department of Civil Engineering, National University (Bali and the Department of Architecture, Udayana University. It examines the conversion of green open spaces, a major problem in Denpasar. The seventh paper is authored by Gusti Ayu Made Suartika. It examines the relevance of multi storey housing in fulfilling the need for affordable houses by low income urban dwellers in Indonesia. Finally, a big thank you to all our contributors, editorial board, production team and publishing staff who have worked faithfully to bring the journal RUANG- SPACE into being.

  5. EDITORIAL

    Directory of Open Access Journals (Sweden)

    Gusti Ayu Made Suartika

    2014-07-01

    . Tulisan Emmi mengkaji tentang pemanfaatan ruang-ruang komunal di Desa Pedungan (Denpasar, wujud perubahan fungsi yang ada dan faktor-faktor penyebab perubahan ini. Artikel keempat ditulis oleh I Made Wirata (arsitek profesional dan Ngakan Putu Sueca (profesor Jurusan Arsitektur, Universitas Udayana. Tulisan ini membahas tentang konsep rumah adat Suku Sasak di Dusun Segenter, Lombok Utara. Keunikan permukiman ini adalah pada penempatan ruang-ruang yang sangat tergantung dari penempatan pintu dan bale sakanem (bangunan dengan 6 tiang struktur. Artikel kelima ditulis oleh Dinar Sukma Pramesti, seorang arsitek profesional. Tulisan Dinar mendiskusikan tentang tipe rumah panggung di Kampung Loloan, Kabupaten Jembrana, Bali. Dinar menggarisbawahi jika bentuk arsitektur rumah panggung kampung ini telah berkembang, menyesuaikan kondisi sosial-budaya, ekonomi, umur bangunan, ketersediaan lahan, dan preferensi dari penghuni. Artikel keenam disusun oleh I Nengah Riana, Widiastuti, dan Ida Bagus Gde Primayatna, staf akademik dari Jurusan Teknik Sipil, Universitas Pendidikan Nasional, (Bali dan Jurusan Arsitektur, Universitas Udayana. Paper ini menstudi salah satu permasalahan serius yang semakin dihadapi Kota Denpasar, yaitu perubahan pemanfaatan ruang terbuka hijau. Perubahan ini dilihat dari wujud perubahannya serta faktor-faktor yang menyebabkan terjadinya alih fungsi ini. Artikel ketujuh mengkaji tentang relevansi dari perumahan berlantai banyak dalam pemenuhan kebutuhan perumahan untuk masyarakat berpendapatan rendah di daerah perkotaan di Indonesia, yang ditulis oleh Gusti Ayu Made Suartika, seorang akademik dari Program Studi Magister Perencanaan dan Pembangunan Spasial Desa/Kota, Universitas Udayana. Akhirnya terima kasih yang sebesar-besarnya disampaikan kepada para penulis, wakil editor, anggota dewan editor, staf editor, dan team produksi, yang telah berkontribusi dan bekerja keras sehingga publikasi Jurnal RUANG-SPACE terealisasikan.