Sample records for automatic colorimetric calibration

  1. Colorimetric calibration of coupled infrared simulation system (United States)

    Zhang, Ying; Fei, Jindong; Gao, Yang; Du, Jian


    In order to test 2-color infrared sensors, a coupled infrared simulation system can generate radiometric outputs with wavelengths that range from less than 3 microns to more than 12 microns. There are two channels in the coupled simulation system, optically combined by a diachronic beam combiner. Each channel has an infrared blackbody, a filter, a diaphragm, and diaphragm-motors. The system is projected to the sensor under testing by a collimator. This makes it difficult to calibrate the system with only one-band thermal imager. Errors will be caused in the radiance levels measured by the narrow band thermal imager. This paper describes colorimetric temperature measurement techniques that have been developed to perform radiometric calibrations of these infrared simulation systems above. The calibration system consists of two infrared thermal imagers; one is operated at the wavelength range of MW-IR, and the other at the range of LW-IR.

  2. Automatic calibration method for plenoptic camera (United States)

    Luan, Yinsen; He, Xing; Xu, Bing; Yang, Ping; Tang, Guomao


    An automatic calibration method is proposed for a microlens-based plenoptic camera. First, all microlens images on the white image are searched and recognized automatically based on digital morphology. Then, the center points of microlens images are rearranged according to their relative position relationships. Consequently, the microlens images are located, i.e., the plenoptic camera is calibrated without the prior knowledge of camera parameters. Furthermore, this method is appropriate for all types of microlens-based plenoptic cameras, even the multifocus plenoptic camera, the plenoptic camera with arbitrarily arranged microlenses, or the plenoptic camera with different sizes of microlenses. Finally, we verify our method by the raw data of Lytro. The experiments show that our method has higher intelligence than the methods published before.

  3. Automatic Calibration Of Manual Machine Tools (United States)

    Gurney, Rex D.


    Modified scheme uses data from multiple positions and eliminates tedious positioning. Modification of computer program adapts calibration system for convenient use with manually-controlled machine tools. Developed for use on computer-controlled tools. Option added to calibration program allows data on random tool-axis positions to be entered manually into computer for reduction. Instead of setting axis to predetermined positions, operator merely sets it at variety of arbitrary positions.

  4. Towards automatic calibration of 2-dimensional flood propagation models

    Directory of Open Access Journals (Sweden)

    P. Fabio


    Full Text Available Hydraulic models for flood propagation description are an essential tool in many fields, e.g. civil engineering, flood hazard and risk assessments, evaluation of flood control measures, etc. Nowadays there are many models of different complexity regarding the mathematical foundation and spatial dimensions available, and most of them are comparatively easy to operate due to sophisticated tools for model setup and control. However, the calibration of these models is still underdeveloped in contrast to other models like e.g. hydrological models or models used in ecosystem analysis. This has basically two reasons: first, the lack of relevant data against the models can be calibrated, because flood events are very rarely monitored due to the disturbances inflicted by them and the lack of appropriate measuring equipment in place. Secondly, especially the two-dimensional models are computationally very demanding and therefore the use of available sophisticated automatic calibration procedures is restricted in many cases. This study takes a well documented flood event in August 2002 at the Mulde River in Germany as an example and investigates the most appropriate calibration strategy for a full 2-D hyperbolic finite element model. The model independent optimiser PEST, that gives the possibility of automatic calibrations, is used. The application of the parallel version of the optimiser to the model and calibration data showed that a it is possible to use automatic calibration in combination of 2-D hydraulic model, and b equifinality of model parameterisation can also be caused by a too large number of degrees of freedom in the calibration data in contrast to a too simple model setup. In order to improve model calibration and reduce equifinality a method was developed to identify calibration data with likely errors that obstruct model calibration.

  5. CCD camera automatic calibration technology and ellipse recognition algorithm

    Institute of Scientific and Technical Information of China (English)

    Changku Sun; Xiaodong Zhang; Yunxia Qu


    A novel two-dimensional (2D) pattern used in camera calibration is presented. With one feature circle located at the center, an array of circles is photo-etched on this pattern. An ellipse recognition algorithm is proposed to implement the acquisition of interest calibration points without human intervention. According to the circle arrangement of the pattern, the relation between three-dimensional (3D) and 2D coordinates of these points can be established automatically and accurately. These calibration points are computed for intrinsic parameters calibration of charge-coupled device (CCD) camera with Tsai method. A series of experiments have shown that the algorithm is robust and reliable with the calibration error less than 0.4 pixel. This new calibration pattern and ellipse recognition algorithm can be widely used in computer vision.

  6. Automatic magnetometer calibration with small space coverage (United States)

    Wahdan, Ahmed

    The use of a standalone Global Navigation Satellite System (GNSS) has proved to be insufficient when navigating indoors or in urban canyons due to multipath or obstruction. Recent technological advances in low cost micro-electro-mechanical system (MEMS) -- based sensors (like accelerometers, gyroscopes and magnetometers) enabled the development of sensor-based navigation systems. Although MEMS sensors are low-cost, lightweight, small size, and have low-power consumption, they have complex error characteristics. Accurate computation of the heading angle (azimuth) is one of the most important aspects of any navigation system. It can be computed either by gyroscopes or magnetometers. Gyroscopes are inertial sensors that can provide the angular rate from which the heading can be calculated, however, their outputs drift with time. Moreover, the accumulated errors due to mathematical integration, performed to obtain the heading angle, lead to large heading errors. On the other hand, magnetometers do not suffer from drift and the calculation of heading does not suffer from error accumulation. They can provide an absolute heading from the magnetic north by sensing the earth's magnetic field. However, magnetometer readings are usually affected by magnetic fields, other than the earth magnetic field, and by other error sources; therefore magnetometer calibration is required to use magnetometer as a reliable source of heading in navigation applications. In this thesis, a framework for fast magnetometer calibration is proposed. This framework requires little space coverage with no user involvement in the calibration process, and does not need specific movements to be performed. The proposed techniques are capable of performing both 2-dimensional (2D) and 3-dimensional (3D) calibration for magnetometers. They are developed to consider different scenarios suitable for different applications, and can benefit from natural device movements. Some applications involve tethering the

  7. The automatic calibration of Korean VLBI Network data

    CERN Document Server

    Hodgson, Jeffrey A; Zhao, Guang-Yao; Algaba, Juan-Carlos; Yun, Youngjoo; Jung, Taehyun; Byun, Do-Young


    The calibration of Very Long Baseline Interferometry (VLBI) data has long been a time consuming process. The Korean VLBI Network (KVN) is a simple array consisting of three identical antennas. Because four frequencies are observed simultaneously, phase solutions can be transferred from lower frequencies to higher frequencies in order to improve phase coherence and hence sensitivity at higher frequencies. Due to the homogeneous nature of the array, the KVN is also well suited for automatic calibration. In this paper we describe the automatic calibration of single-polarisation KVN data using the KVN Pipeline and comparing the results against VLBI data that has been manually reduced. We find that the pipelined data using phase transfer produces better results than a manually reduced dataset not using the phase transfer. Additionally we compared the pipeline results with a manually reduced phase-transferred dataset and found the results to be identical.

  8. The Automatic Calibration of Korean VLBI Network Data (United States)

    Hodgson, Jeffrey A.; Lee, Sang-Sung; Zhao, Guang-Yao; Algaba, Juan-Carlos; Yun, Youngjoo; Jung, Taehyun; Byun, Do-Young


    The calibration of Very Long Baseline Interferometry (VLBI) data has long been a time consuming process. The Korean VLBI Network (KVN) is a simple array consisting of three identical antennas. Because four frequencies are observed simultaneously, phase solutions can be transferred from lower frequencies to higher frequencies in order to improve phase coherence and hence sensitivity at higher frequencies. Due to the homogeneous nature of the array, the KVN is also well suited for automatic calibration. In this paper we describe the automatic calibration of single-polarisation KVN data using the KVN Pipeline and comparing the results against VLBI data that has been manually reduced. We find that the pipelined data using phase transfer produces better results than a manually reduced dataset not using the phase transfer. Additionally we compared the pipeline results with a manually reduced phase-transferred dataset and found the results to be identical.

  9. Automatic Chessboard Detection for Intrinsic and Extrinsic Camera Parameter Calibration

    Directory of Open Access Journals (Sweden)

    Jose María Armingol


    Full Text Available There are increasing applications that require precise calibration of cameras to perform accurate measurements on objects located within images, and an automatic algorithm would reduce this time consuming calibration procedure. The method proposed in this article uses a pattern similar to that of a chess board, which is found automatically in each image, when no information regarding the number of rows or columns is supplied to aid its detection. This is carried out by means of a combined analysis of two Hough transforms, image corners and invariant properties of the perspective transformation. Comparative analysis with more commonly used algorithms demonstrate the viability of the algorithm proposed, as a valuable tool for camera calibration.

  10. Colorimetric Analysis Using Scene-Adaptive Color Conversion Matrix of Calibrated CIS

    Directory of Open Access Journals (Sweden)

    Sung-Hak Lee


    Full Text Available The RGB signals of different CISs (color image sensors do not register the same values for the same viewing scene owing to their different spectral sensitivity and white balance mechanisms. Thus, CISs must be characterized based on CIE standard observation for colorimetric purposes. One general method for characterizing CISs is least square polynomial modeling to derive the colorimetric transfer matrix between RGB outputs and CIE XYZ tristimulus inputs. However, the transfer matrix that is obtained under the standard CIE illumination is unable to estimate various conditions of a CIS that is operated under various illuminations with varying chromaticity and luminance. Therefore, repeated experiments are necessary to obtain accurate colorimetric analysis results. This paper presents a scene-adaptive colorimetric analysis method using images captured by a general consumer camera under various environments.

  11. Desktop calibration of automatic transmission for passenger vehicle

    Institute of Scientific and Technical Information of China (English)

    FANG Chi; SHI Jian-peng; WANG Jun


    Desktop calibration of automatic transmission (AT) is a method which can reduce cost, enhance efficiency and shorten the development periods of a vehicle effectively. We primary introduced the principle and approach of desktop calibration of AT based on the condition of coupling characteristics between engine and torque converter and obtained right point exactly. It is shown to agree with experimental measurements reasonably well. It was used in different applications abroad based on AT technology and achieved a good performance of the vehicle compared with traditional AT technology which primary focuses on the drivability, performance and fuel consumption.

  12. Automatic Camera Calibration Using Active Displays of a Virtual Pattern. (United States)

    Tan, Lei; Wang, Yaonan; Yu, Hongshan; Zhu, Jiang


    Camera calibration plays a critical role in 3D computer vision tasks. The most commonly used calibration method utilizes a planar checkerboard and can be done nearly fully automatically. However, it requires the user to move either the camera or the checkerboard during the capture step. This manual operation is time consuming and makes the calibration results unstable. In order to solve the above problems caused by manual operation, this paper presents a full-automatic camera calibration method using a virtual pattern instead of a physical one. The virtual pattern is actively transformed and displayed on a screen so that the control points of the pattern can be uniformly observed in the camera view. The proposed method estimates the camera parameters from point correspondences between 2D image points and the virtual pattern. The camera and the screen are fixed during the whole process; therefore, the proposed method does not require any manual operations. Performance of the proposed method is evaluated through experiments on both synthetic and real data. Experimental results show that the proposed method can achieve stable results and its accuracy is comparable to the standard method by Zhang.

  13. Calibration of the Hydrological Simulation Program Fortran (HSPF) model using automatic calibration and geographical information systems (United States)

    Al-Abed, N. A.; Whiteley, H. R.


    Calibrating a comprehensive, multi-parameter conceptual hydrological model, such as the Hydrological Simulation Program Fortran model, is a major challenge. This paper describes calibration procedures for water-quantity parameters of the HSPF version 10·11 using the automatic-calibration parameter estimator model coupled with a geographical information system (GIS) approach for spatially averaged properties. The study area was the Grand River watershed, located in southern Ontario, Canada, between 79° 30 and 80° 57W longitude and 42° 51 and 44° 31N latitude. The drainage area is 6965 km2. Calibration efforts were directed to those model parameters that produced large changes in model response during sensitivity tests run prior to undertaking calibration. A GIS was used extensively in this study. It was first used in the watershed segmentation process. During calibration, the GIS data were used to establish realistic starting values for the surface and subsurface zone parameters LZSN, UZSN, COVER, and INFILT and physically reasonable ratios of these parameters among watersheds were preserved during calibration with the ratios based on the known properties of the subwatersheds determined using GIS. This calibration procedure produced very satisfactory results; the percentage difference between the simulated and the measured yearly discharge ranged between 4 to 16%, which is classified as good to very good calibration. The average simulated daily discharge for the watershed outlet at Brantford for the years 1981-85 was 67 m3 s-1 and the average measured discharge at Brantford was 70 m3 s-1. The coupling of a GIS with automatice calibration produced a realistic and accurate calibration for the HSPF model with much less effort and subjectivity than would be required for unassisted calibration.

  14. Automatic calibration and neural networks for robot guidance (United States)

    Sethuramasamyraja, Balaji; Ghaffari, Masoud; Hall, Ernest L.


    An autonomous robot must be able to sense its environment and react appropriately in a variable environment. The University of Cincinnati Robot team is actively involved in building a small, unmanned, autonomously guided vehicle for the International Ground Robotics Contest organized by Association for Unmanned Vehicle Systems International (AUVSI) each year. The unmanned vehicle is supposed to follow an obstacle course bounded by two white/yellow lines, which are four inches thick and 10 feet apart. The navigation system for one of the University of Cincinnati"s designs, Bearcat, uses 2 CCD cameras and an image-tracking device for the front end processing of the image captured by the cameras. The three dimensional world co-ordinates were reduced to two dimensional image coordinates as a result of the transformations taking place from the ground plane to the image plane. A novel automatic calibration system was designed to transform the image co-ordinates back to world co-ordinates for navigation purposes. The purpose of this paper is to simplify this tedious calibration using an artificial neural network. Image processing is used to automatically detect calibration points. Then a back projection neural algorithm is used to learn the relationships between the image coordinates and three-dimensional coordinates. This transformation is the main focus of this study. Using these algorithms, the robot built with this design is able to track and follow the lines successfully.

  15. VSHEC—A program for the automatic spectrum calibration (United States)

    Zlokazov, V. B.; Utyonkov, V. K.; Tsyganov, Yu. S.


    Calibration is the transformation of the output channels of a measuring device into the physical values (energies, times, angles, etc.). If dealt with manually, it is a labor- and time-consuming procedure even if only a few detectors are used. However, the situation changes appreciably if a calibration of multi-detector systems is required, where the number of registering devices extends to hundreds (Tsyganov et al. (2004) [1]). The calibration is aggravated by the fact that needed pivotal channel numbers should be determined from peak-like distributions. But peak distribution is an informal pattern so that a procedure of pattern recognition should be employed to discard the operator interference. The automatic calibration is the determination of the calibration curve parameters on the basis of reference quantity list and the data which partially are characterized by these quantities (energies, angles, etc). The program allows the physicist to perform the calibration of the spectrometric detectors for both the cases: that of one tract and that of many. Program summaryProgram title: VSHEC Catalogue identifier: AENN_v1_0 Program summary URL: Program obtainable from: CPC Program Library, Queen's University, Belfast, N. Ireland Licensing provisions: Standard CPC licence, No. of lines in distributed program, including test data, etc.: 6403 No. of bytes in distributed program, including test data, etc.: 325847 Distribution format: tar.gz Programming language: DELPHI-5 and higher. Computer: Any IBM PC compatible. Operating system: Windows XX. Classification: 2.3, 4.9. Nature of problem: Automatic conversion of detector channels into their energy equivalents. Solution method: Automatic decomposition of a spectrum into geometric figures such as peaks and an envelope of peaks from below, estimation of peak centers and search for the maximum peak center subsequence which matches the

  16. [Automatization of colorimetric serum zinc determination using the Bayer RA 1000 autoanalyzer]. (United States)

    Shum-Cheong-Sing, J; Arnaud, J; Favier, A


    A colorimetric method for the determination of zinc in serum was adapted for use with the Bayer RA 1000 autoanalyzer. Zinc is dissociated from proteins by guanidinium hydrochloride and complexed with 2-(5-bromo-2-pyridylazo)-5-(N-propyl-N- sulfopropylamino-)phenol (5-Br-PAPS). Precision (within-run CVs from 1.4 to 4.2%; between-run CVs from 3.5 to 8.1%), accuracy (93 to 103%) and detection limit (1.15 mumol/l) were sufficient for routine determinations. Moreover, results obtained with the proposed Bayer RA 1000 method were in good agreement with those obtained by flame atomic absorption spectrometry (FAAS) which could be considered the reference method. For 119 sera, the correlation coefficient r was found to be 0.958 and the regression line was as follows: serum zinc (RA 1000) = 0.96 serum zinc (FAAS) + 0.8 mumol/l. Moreover, 98% of the differences between colorimetry and FAAS were within the range Md +/- 1.96 SDd, where Md is the mean of the differences and SDd is the standard deviation of the differences.

  17. Improving the automatic wavelength calibration of EMIR spectroscopic data (United States)

    Cardiel, N.; Pascual, S.; Picazo, P.; Gallego, J.; Garzón, F.; Castro-Rodríguez, N.; González-Fernández, C.; Hammersley, P.; Insausti, M.; Manjavacas, E.; Miluzio, M.


    EMIR, the near-infrared camera-spectrograph operating in the near-infrared (NIR) wavelengths 0.9-2.5μm, is being commissioned at the Nasmyth focus of the Gran Telescopio CANARIAS. One of the most outstanding capabilities of EMIR will be its multi-object spectroscopic mode which, with the help of a robotic reconfigurable slit system, will allow to take around 53 spectra simultaneously. A data reduction pipeline, PyEmir, based on Python, is being developed in order to facilitate the automatic reduction of EMIR data taken in both imaging and spectroscopy mode. Focusing on the reduction of spectroscopic data, some critical manipulations include the geometric distortion correction and the wavelength calibration. Although usually these reductions steps are carried out separately, it is important to realise that these kind of manipulations involve data rebinning and interpolation, which in addition unavoidably lead to the increase of error correlation and to resolution degradation. In order to minimise these effects, it is possible to incorporate those data manipulations as a single geometric transformation. This approach is being used in the development of PyEmir. For this purpose, the geometric transformations available in the Python package Scikit-image are being used. This work was funded by the Spanish Programa Nacional de Astronomía y Astrofísica under grant AYA2013-46724-P.

  18. Development of an automatic calibration device for high-accuracy low temperature thermometers

    Institute of Scientific and Technical Information of China (English)


    Based on the analysis and investigation of calibration systems for high-accuracy low temperature thermometers,a new facility for automatic calibration of high-accuracy low temperature thermometers was developed.Continuous calibration for multiple points can be made automatically with this device.According to the thermophysical characteristics of the constant-temperature block in this device,segmented Fuzzy-PID (proportional-integral-differential) algorithm was applied.The experimental results showed that the temperature fluctuation was smaller than ±0.005 K in 30 min.Therefore,this new device can fully meet the calibration requirement of high-precision low temperature thermometers.

  19. Calibration Method of Multi Parameter Water Quality Analyzer by Colorimetric Method%比色法多参数水质分析仪的校准方法

    Institute of Scientific and Technical Information of China (English)



    随着水质分析技术的不断发展,比色法多参数水质分析仪被广泛应用于环境污染中的水质分析、工业生产中的废水水质分析。通过对比色法多参数水质分析仪的校准,可以很好地控制在使用比色法多参数水质分析仪进行实验分析过程中带来的分析误差。结合平时的工作积累,就比色法多参数水质分析仪计量特性校准方法进行讨论。%With the development of water quality analysis,water quality analyzer based on colorimetric method is widely used in environmental pollution of water quality analysis and industrial wastewater analysis.The calibration of water quality analyzer based on colorimetric method made analytical error can be controlled in the process of the experimental analysis.Discuss the calibration method of water quality analyzer based on colorimetric method that connecting with work experience.

  20. Calibration of three rainfall simulators with automatic measurement methods (United States)

    Roldan, Margarita


    CALIBRATION OF THREE RAINFALL SIMULATORS WITH AUTOMATIC MEASUREMENT METHODS M. Roldán (1), I. Martín (2), F. Martín (2), S. de Alba(3), M. Alcázar(3), F.I. Cermeño(3) 1 Grupo de Investigación Ecología y Gestión Forestal Sostenible. ECOGESFOR-Universidad Politécnica de Madrid. E.U.I.T. Forestal. Avda. Ramiro de Maeztu s/n. Ciudad Universitaria. 28040 Madrid. 2 E.U.I.T. Forestal. Avda. Ramiro de Maeztu s/n. Ciudad Universitaria. 28040 Madrid. 3 Facultad de Ciencias Geológicas. Universidad Complutense de Madrid. Ciudad Universitaria s/n. 28040 Madrid The rainfall erosivity is the potential ability of rain to cause erosion. It is function of the physical characteristics of rainfall (Hudson, 1971). Most expressions describing erosivity are related to kinetic energy or momentum and so with drop mass or size and fall velocity. Therefore, research on factors determining erosivity leds to the necessity to study the relation between fall height and fall velocity for different drop sizes, generated in a rainfall simulator (Epema G.F.and Riezebos H.Th, 1983) Rainfall simulators are one of the most used tools for erosion studies and are used to determine fall velocity and drop size. Rainfall simulators allow repeated and multiple measurements The main reason for use of rainfall simulation as a research tool is to reproduce in a controlled way the behaviour expected in the natural environment. But in many occasions when simulated rain is used in order to compare it with natural rain, there is a lack of correspondence between natural and simulated rain and this can introduce some doubt about validity of data because the characteristics of natural rain are not adequately represented in rainfall simulation research (Dunkerley D., 2008). Many times the rainfall simulations have high rain rates and they do not resemble natural rain events and these measures are not comparables. And besides the intensity is related to the kinetic energy which

  1. Automatic online spectral calibration of Fourier-domain OCT for robotic surgery (United States)

    Liu, Xuan; Balicki, Marcin; Taylor, Russell H.; Kang, Jin U.


    We present a new automatic spectral calibration (ASC) method for spectral Domain optical coherence tomography (SD-OCT). Our ASC method calibrates the spectral mapping of the spectrometer in SD-OCT, and does not require external calibrating light source or a commercial spectral analyzer. The ASC method simultaneously calibrates the physical pixel spacing of the A-scan in static and dynamic environments. Experimental results show that the proposed ASC method can provide satisfactory calibration for SD-OCT to achieve high axial resolution and high ranging accuracy, without increasing hardware complexity.

  2. 3D Surface Reconstruction and Automatic Camera Calibration (United States)

    Jalobeanu, Andre


    Illustrations in this view-graph presentation are presented on a Bayesian approach to 3D surface reconstruction and camera calibration.Existing methods, surface analysis and modeling,preliminary surface reconstruction results, and potential applications are addressed.

  3. Automatic Calibration and Reconstruction for Active Vision Systems

    CERN Document Server

    Zhang, Beiwei


    In this book, the design of two new planar patterns for camera calibration of intrinsic parameters is addressed and a line-based method for distortion correction is suggested. The dynamic calibration of structured light systems, which consist of a camera and a projector is also treated. Also, the 3D Euclidean reconstruction by using the image-to-world transformation is investigated. Lastly, linear calibration algorithms for the catadioptric camera are considered, and the homographic matrix and fundamental matrix are extensively studied. In these methods, analytic solutions are provided for the computational efficiency and redundancy in the data can be easily incorporated to improve reliability of the estimations. This volume will therefore prove valuable and practical tool for researchers and practioners working in image processing and computer vision and related subjects.

  4. Calibration of an Automatic System Using a Laser Signature

    Directory of Open Access Journals (Sweden)

    Edward F. Plinski


    Full Text Available The specific phenomenon, which appears in tuned CO2 lasers, called a laser signature, is used as a standard for calibration of the servomechanism. The proposed servomechanism can be used for continuous investigations of the laser signatures of different laser media.

  5. Spectral phase-based automatic calibration scheme for swept source-based optical coherence tomography systems (United States)

    Ratheesh, K. M.; Seah, L. K.; Murukeshan, V. M.


    The automatic calibration in Fourier-domain optical coherence tomography (FD-OCT) systems allows for high resolution imaging with precise depth ranging functionality in many complex imaging scenarios, such as microsurgery. However, the accuracy and speed of the existing automatic schemes are limited due to the functional approximations and iterative operations used in their procedures. In this paper, we present a new real-time automatic calibration scheme for swept source-based optical coherence tomography (SS-OCT) systems. The proposed automatic calibration can be performed during scanning operation and does not require an auxiliary interferometer for calibration signal generation and an additional channel for its acquisition. The proposed method makes use of the spectral component corresponding to the sample surface reflection as the calibration signal. The spectral phase function representing the non-linear sweeping characteristic of the frequency-swept laser source is determined from the calibration signal. The phase linearization with improved accuracy is achieved by normalization and rescaling of the obtained phase function. The fractional-time indices corresponding to the equidistantly spaced phase intervals are estimated directly from the resampling function and are used to resample the OCT signals. The proposed approach allows for precise calibration irrespective of the path length variation induced by the non-planar topography of the sample or galvo scanning. The conceived idea was illustrated using an in-house-developed SS-OCT system by considering the specular reflection from a mirror and other test samples. It was shown that the proposed method provides high-performance calibration in terms of axial resolution and sensitivity without increasing computational and hardware complexity.

  6. [Full-field and automatic methodology of spectral calibration for PGP imaging spectrometer]. (United States)

    Sun, Ci; Bayanheshig; Cui, Ji-cheng; Pan, Ming-zhong; Li, Xiao-tian; Tang, Yu-guo


    In order to analyze spectral data quantitatively which is obtained by prism-grating-prism imaging spectrometer, spectral calibration is required in order to determine spectral characteristics of PGP imaging spectrometer, such as the center wavelength of every spectral channel, spectral resolution and spectral bending. A spectral calibration system of full field based on collimated monochromatic light method is designed. Spherical mirror is used to provide collimated light, and a freely sliding and rotating folding mirror is adopted to change the angle of incident light in order to realize full field and automatic calibration of imaging spectrometer. Experiments of spectral calibration have been done for PGP imaging spectrometer to obtain parameters of spectral performance, and accuracy analysis combined with the structural features of the entire spectral calibration system have been done. Analysis results indicate that spectral calibration accuracy of the calibration system reaches 0.1 nm, and the bandwidth accuracy reaches 1.3%. The calibration system has merits of small size, better commonality, high precision and so on, and because of adopting the control of automation, the additional errors which are caused by human are avoided. The calibration system can be used for spectral calibration of other imaging spectrometers whose structures are similar to PGP.

  7. Object Occlusion Detection Using Automatic Camera Calibration for a Wide-Area Video Surveillance System. (United States)

    Jung, Jaehoon; Yoon, Inhye; Paik, Joonki


    This paper presents an object occlusion detection algorithm using object depth information that is estimated by automatic camera calibration. The object occlusion problem is a major factor to degrade the performance of object tracking and recognition. To detect an object occlusion, the proposed algorithm consists of three steps: (i) automatic camera calibration using both moving objects and a background structure; (ii) object depth estimation; and (iii) detection of occluded regions. The proposed algorithm estimates the depth of the object without extra sensors but with a generic red, green and blue (RGB) camera. As a result, the proposed algorithm can be applied to improve the performance of object tracking and object recognition algorithms for video surveillance systems.

  8. Automatic calibration of damping layers in finite element time domain simulations


    Vandekerckhove, Steven; Wells, Garth N.; De Gersem, Herbert; Abeele, Koen Van Den


    Matched layers are commonly used in numerical simulations of wave propagation to model (semi-)infinite domains. Attenuation functions describe the damping in layers, and provide a matching of the wave impedance at the interface between the domain of interest and the absorbing region. Selecting parameters in the attenuation functions is non-trivial. In this work, an optimisation procedure for automatically calibrating matched layers is presented. The procedure is based on solving optimisation ...

  9. A new automatic system for angular measurement and calibration in radiometric instruments. (United States)

    Marquez, Jose Manuel Andujar; Bohórquez, Miguel Ángel Martínez; Garcia, Jonathan Medina; Nieto, Francisco Jose Aguilar


    This paper puts forward the design, construction and testing of a new automatic system for angular-response measurement and calibration in radiometric instruments. Its main characteristics include precision, speed, resolution, noise immunity, easy programming and operation. The developed system calculates the cosine error of the radiometer under test by means of a virtual instrument, from the measures it takes and through a mathematical procedure, thus allowing correcting the radiometer with the aim of preventing cosine error in its measurements.

  10. A New Automatic System for Angular Measurement and Calibration in Radiometric Instruments

    Directory of Open Access Journals (Sweden)

    Jose Manuel Andujar Marquez


    Full Text Available This paper puts forward the design, construction and testing of a new automatic system for angular-response measurement and calibration in radiometric instruments. Its main characteristics include precision, speed, resolution, noise immunity, easy programming and operation. The developed system calculates the cosine error of the radiometer under test by means of a virtual instrument, from the measures it takes and through a mathematical procedure, thus allowing correcting the radiometer with the aim of preventing cosine error in its measurements.


    Directory of Open Access Journals (Sweden)

    M. Hassanein


    Full Text Available In the last few years, multi-cameras and LIDAR systems draw the attention of the mapping community. They have been deployed on different mobile mapping platforms. The different uses of these platforms, especially the UAVs, offered new applications and developments which require fast and accurate results. The successful calibration of such systems is a key factor to achieve accurate results and for the successful processing of the system measurements especially with the different types of measurements provided by the LIDAR and the cameras. The system calibration aims to estimate the geometric relationships between the different system components. A number of applications require the systems be ready for operation in a short time especially for disasters monitoring applications. Also, many of the present system calibration techniques are constrained with the need of special arrangements in labs for the calibration procedures. In this paper, a new technique for calibration of integrated LIDAR and multi-cameras systems is presented. The new proposed technique offers a calibration solution that overcomes the need for special labs for standard calibration procedures. In the proposed technique, 3D reconstruction of automatically detected and matched image points is used to generate a sparse images-driven point cloud then, a registration between the LIDAR generated 3D point cloud and the images-driven 3D point takes place to estimate the geometric relationships between the cameras and the LIDAR.. In the presented technique a simple 3D artificial target is used to simplify the lab requirements for the calibration procedure. The used target is composed of three intersected plates. The choice of such target geometry was to ensure enough conditions for the convergence of registration between the constructed 3D point clouds from the two systems. The achieved results of the proposed approach prove its ability to provide an adequate and fully automated

  12. a New Automatic System Calibration of Multi-Cameras and LIDAR Sensors (United States)

    Hassanein, M.; Moussa, A.; El-Sheimy, N.


    In the last few years, multi-cameras and LIDAR systems draw the attention of the mapping community. They have been deployed on different mobile mapping platforms. The different uses of these platforms, especially the UAVs, offered new applications and developments which require fast and accurate results. The successful calibration of such systems is a key factor to achieve accurate results and for the successful processing of the system measurements especially with the different types of measurements provided by the LIDAR and the cameras. The system calibration aims to estimate the geometric relationships between the different system components. A number of applications require the systems be ready for operation in a short time especially for disasters monitoring applications. Also, many of the present system calibration techniques are constrained with the need of special arrangements in labs for the calibration procedures. In this paper, a new technique for calibration of integrated LIDAR and multi-cameras systems is presented. The new proposed technique offers a calibration solution that overcomes the need for special labs for standard calibration procedures. In the proposed technique, 3D reconstruction of automatically detected and matched image points is used to generate a sparse images-driven point cloud then, a registration between the LIDAR generated 3D point cloud and the images-driven 3D point takes place to estimate the geometric relationships between the cameras and the LIDAR.. In the presented technique a simple 3D artificial target is used to simplify the lab requirements for the calibration procedure. The used target is composed of three intersected plates. The choice of such target geometry was to ensure enough conditions for the convergence of registration between the constructed 3D point clouds from the two systems. The achieved results of the proposed approach prove its ability to provide an adequate and fully automated calibration without

  13. Automatic Calibration of Frequency Compensation System in Computer-Controlled Patch-Clamp Amplifier

    Directory of Open Access Journals (Sweden)

    Jun Xion


    Full Text Available Computer-controlled patch-clamp amplifier is a digitally controlled analog device used to record the cellular ion channel currents in electrophysiology research. The inherent bandwidth and performance of the headstage is limited by the stray capacitance and distributed capacitance across the feedback resistors. In order to effectively improve the performance of the headstage, the paper advanced a simplified automatic calibration method of frequency compensation system in resistor-feedback patch-clamp amplifier. The dynamic model of headstage was approximate as a two poles and one zero system in the transfer function by experience and test results, so the dynamic characteristics of the headstage were obtained employing least squares parameter estimation algorithm. Further more, the compensation parameter of high frequency booster can be estimated by the time constant of main pole of headstage. And automatic adjustment of the parameters in transient response correction stage was performed as a least squares fitting problem. The software routine running on the host computer conducted all operations of frequency compensation. Experimental results demonstrate that the simplified automatic calibration method can substantially extend the bandwidth and minimize step response error of headstage.

  14. Multi-objective Automatic Calibration of a Semi-distributed Watershed Model Using Pareto Ordering Optimization and Genetic Algorithm (United States)

    This study explored the application of a multi-objective evolutionary algorithm (MOEA) and Pareto ordering in the multiple-objective automatic calibration of the Soil and Water Assessment Tool (SWAT). SWAT was calibrated in the Calapooia watershed, Oregon, USA, with two different pairs of objective ...

  15. Colorimetric Determination of the Iron(III)-Thiocyanate Reaction Equilibrium Constant with Calibration and Equilibrium Solutions Prepared in a Cuvette by Sequential Additions of One Reagent to the Other (United States)

    Nyasulu, Frazier; Barlag, Rebecca


    The well-known colorimetric determination of the equilibrium constant of the iron(III-thiocyanate complex is simplified by preparing solutions in a cuvette. For the calibration plot, 0.10 mL increments of 0.00100 M KSCN are added to 4.00 mL of 0.200 M Fe(NO[subscript 3])[subscript 3], and for the equilibrium solutions, 0.50 mL increments of…

  16. Modeling Earthen Dike Stability: Sensitivity Analysis and Automatic Calibration of Diffusivities Based on Live Sensor Data

    CERN Document Server

    Melnikova, N B; Sloot, P M A


    The paper describes concept and implementation details of integrating a finite element module for dike stability analysis Virtual Dike into an early warning system for flood protection. The module operates in real-time mode and includes fluid and structural sub-models for simulation of porous flow through the dike and for dike stability analysis. Real-time measurements obtained from pore pressure sensors are fed into the simulation module, to be compared with simulated pore pressure dynamics. Implementation of the module has been performed for a real-world test case - an earthen levee protecting a sea-port in Groningen, the Netherlands. Sensitivity analysis and calibration of diffusivities have been performed for tidal fluctuations. An algorithm for automatic diffusivities calibration for a heterogeneous dike is proposed and studied. Analytical solutions describing tidal propagation in one-dimensional saturated aquifer are employed in the algorithm to generate initial estimates of diffusivities.

  17. Poster Abstract: Automatic Calibration of Device Attitude in Inertial Measurement Unit Based Traffic Probe Vehicles

    KAUST Repository

    Mousa, Mustafa


    Probe vehicles consist in mobile traffic sensor networks that evolve with the flow of vehicles, transmitting velocity and position measurements along their path, generated using GPSs. To address the urban positioning issues of GPSs, we propose to replace them with inertial measurement units onboard vehicles, to estimate vehicle location and attitude using inertial data only. While promising, this technology requires one to carefully calibrate the orientation of the device inside the vehicle to be able to process the acceleration and rate gyro data. In this article, we propose a scheme that can perform this calibration automatically by leveraging the kinematic constraints of ground vehicles, and that can be implemented on low-end computational platforms. Preliminary testing shows that the proposed scheme enables one to accurately estimate the actual accelerations and rotation rates in the vehicle coordinates. © 2016 IEEE.

  18. A digitally calibrated CMOS RMS power detector for RF automatic gain control

    Institute of Scientific and Technical Information of China (English)

    Yan Taotao; Wang Hui; Li Jinbo; Zhou Jianjun


    This paper presents the design and implementation of a digitally calibrated CMOS wideband radio frequency (RF) root-mean-square (RMS) power detector for high accuracy RF automatic gain control (AGC).The proposed RMS power detector demonstrates accurate power detection in the presence of process,supply voltage,and temperature (PVT) variations by employing a digital calibration scheme.It also consumes low power and occupies a small chip area.The measurement results show that the scheme improves the accuracy of the detector to better than 0.3 dB over the PVT variations and wide operating frequency range from 0.2 to 0.8 GHz.Implemented in a 0.18 μm CMOS process and occupying a small die area of 263 × 214 μm2,the proposed digitally calibrated CMOS RMS power detector only consumes 1.6 mA in power detection mode and 2.1 mA in digital calibration mode from a 1.8 V supply voltage.

  19. Automatic calibration of a global flow routing model in the Amazon basin using virtual SWOT data (United States)

    Rogel, P. Y.; Mouffe, M.; Getirana, A.; Ricci, S. M.; Lion, C.; Mognard, N. M.; Biancamaria, S.; Boone, A.


    The Surface Water and Ocean Topography (SWOT) wide swath altimetry mission will provide a global coverage of surface water elevation, which will be used to help correct water height and discharge prediction from hydrological models. Here, the aim is to investigate the use of virtually generated SWOT data to improve water height and discharge simulation using calibration of model parameters (like river width, river depth and roughness coefficient). In this work, we use the HyMAP model to estimate water height and discharge on the Amazon catchment area. Before reaching the river network, surface and subsurface runoff are delayed by a set of linear and independent reservoirs. The flow routing is performed by the kinematic wave equation.. Since the SWOT mission has not yet been launched, virtual SWOT data are generated with a set of true parameters for HyMAP as well as measurement errors from a SWOT data simulator (i.e. a twin experiment approach is implemented). These virtual observations are used to calibrate key parameters of HyMAP through the minimization of a cost function defining the difference between the simulated and observed water heights over a one-year simulation period. The automatic calibration procedure is achieved using the MOCOM-UA multicriteria global optimization algorithm as well as the local optimization algorithm BC-DFO that is considered as a computational cost saving alternative. First, to reduce the computational cost of the calibration procedure, each spatially distributed parameter (Manning coefficient, river width and river depth) is corrupted through the multiplication of a spatially uniform factor that is the only factor optimized. In this case, it is shown that, when the measurement errors are small, the true water heights and discharges are easily retrieved. Because of equifinality, the true parameters are not always identified. A spatial correction of the model parameters is then investigated and the domain is divided into 4 regions

  20. Design of an expert system to automatically calibrate impedance control for powered knee prostheses. (United States)

    Wang, Ding; Liu, Ming; Zhang, Fan; Huang, He


    Many currently available powered knee prostheses (PKP) use finite state impedance control to operate a prosthetic knee joint. The desired impedance values were usually manually calibrated with trial-and-error in order to enable near-normal walking pattern. However, such a manual approach is inaccurate, time consuming, and impractical. This paper aimed to design an expert system that can tune the control impedance for powered knee prostheses automatically and quickly. The expert system was designed based on fuzzy logic inference (FLI) to match the desired knee motion and gait timing while walking. The developed system was validated on an able-bodied subject wearing a powered prosthesis. Preliminary experimental results demonstrated that the developed expert system can converge the user's knee profile and gait timing to the desired values within 2 minutes. Additionally, after the auto-tuning procedure, the user produced more symmetrical gait. These preliminary results indicate the promise of the designed expert system for quick and accuracy impedance calibration, which can significantly improve the practical value of powered lower limb prosthesis. Continuous engineering efforts are still needed to determine the calibration objectives and validate the expert system.

  1. Using automatic calibration method for optimizing the performance of Pedotransfer functions of saturated hydraulic conductivity

    Directory of Open Access Journals (Sweden)

    Ahmed M. Abdelbaki


    Full Text Available Pedotransfer functions (PTFs are an easy way to predict saturated hydraulic conductivity (Ksat without measurements. This study aims to auto calibrate 22 PTFs. The PTFs were divided into three groups according to its input requirements and the shuffled complex evolution algorithm was used in calibration. The results showed great modification in the performance of the functions compared to the original published functions. For group 1 PTFs, the geometric mean error ratio (GMER and the geometric standard deviation of error ratio (GSDER values were modified from range (1.27–6.09, (5.2–7.01 to (0.91–1.15, (4.88–5.85 respectively. For group 2 PTFs, the GMER and the GSDER values were modified from (0.3–1.55, (5.9–12.38 to (1.00–1.03, (5.5–5.9 respectively. For group 3 PTFs, the GMER and the GSDER values were modified from (0.11–2.06, (5.55–16.42 to (0.82–1.01, (5.1–6.17 respectively. The result showed that the automatic calibration is an efficient and accurate method to enhance the performance of the PTFs.

  2. Temporal Analysis and Automatic Calibration of the Velodyne HDL-32E LiDAR System (United States)

    Chan, T. O.; Lichti, D. D.; Belton, D.


    At the end of the first quarter of 2012, more than 600 Velodyne LiDAR systems had been sold worldwide for various robotic and high-accuracy survey applications. The ultra-compact Velodyne HDL-32E LiDAR has become a predominant sensor for many applications that require lower sensor size/weight and cost. For high accuracy applications, cost-effective calibration methods with minimal manual intervention are always desired by users. However, the calibrations are complicated by the Velodyne LiDAR's narrow vertical field of view and the very highly time-variant nature of its measurements. In the paper, the temporal stability of the HDL-32E is first analysed as the motivation for developing a new, automated calibration method. This is followed by a detailed description of the calibration method that is driven by a novel segmentation method for extracting vertical cylindrical features from the Velodyne point clouds. The proposed segmentation method utilizes the Velodyne point cloud's slice-like nature and first decomposes the point clouds into 2D layers. Then the layers are treated as 2D images and are processed with the Generalized Hough Transform which extracts the points distributed in circular patterns from the point cloud layers. Subsequently, the vertical cylindrical features can be readily extracted from the whole point clouds based on the previously extracted points. The points are passed to the calibration that estimates the cylinder parameters and the LiDAR's additional parameters simultaneously by constraining the segmented points to fit to the cylindrical geometric model in such a way the weighted sum of the adjustment residuals are minimized. The proposed calibration is highly automatic and this allows end users to obtain the time-variant additional parameters instantly and frequently whenever there are vertical cylindrical features presenting in scenes. The methods were verified with two different real datasets, and the results suggest that up to 78

  3. Multiobjective Optimal Algorithm for Automatic Calibration of Daily Streamflow Forecasting Model

    Directory of Open Access Journals (Sweden)

    Yi Liu


    Full Text Available Single-objection function cannot describe the characteristics of the complicated hydrologic system. Consequently, it stands to reason that multiobjective functions are needed for calibration of hydrologic model. The multiobjective algorithms based on the theory of nondominate are employed to solve this multiobjective optimal problem. In this paper, a novel multiobjective optimization method based on differential evolution with adaptive Cauchy mutation and Chaos searching (MODE-CMCS is proposed to optimize the daily streamflow forecasting model. Besides, to enhance the diversity performance of Pareto solutions, a more precise crowd distance assigner is presented in this paper. Furthermore, the traditional generalized spread metric (SP is sensitive with the size of Pareto set. A novel diversity performance metric, which is independent of Pareto set size, is put forward in this research. The efficacy of the new algorithm MODE-CMCS is compared with the nondominated sorting genetic algorithm II (NSGA-II on a daily streamflow forecasting model based on support vector machine (SVM. The results verify that the performance of MODE-CMCS is superior to the NSGA-II for automatic calibration of hydrologic model.

  4. Automatic Calibration Method of Voxel Size for Cone-beam 3D-CT Scanning System

    CERN Document Server

    Yang, Min; Liu, Yipeng; Men, Fanyong; Li, Xingdong; Liu, Wenli; Wei, Dongbo


    For cone-beam three-dimensional computed tomography (3D-CT) scanning system, voxel size is an important indicator to guarantee the accuracy of data analysis and feature measurement based on 3D-CT images. Meanwhile, the voxel size changes with the movement of the rotary table along X-ray direction. In order to realize the automatic calibration of the voxel size, a new easily-implemented method is proposed. According to this method, several projections of a spherical phantom are captured at different imaging positions and the corresponding voxel size values are calculated by non-linear least square fitting. Through these interpolation values, a linear equation is obtained, which reflects the relationship between the rotary table displacement distance from its nominal zero position and the voxel size. Finally, the linear equation is imported into the calibration module of the 3D-CT scanning system, and when the rotary table is moving along X-ray direction, the accurate value of the voxel size is dynamically expo...

  5. An investigation of automatic exposure control calibration for chest imaging with a computed radiography system. (United States)

    Moore, C S; Wood, T J; Avery, G; Balcam, S; Needler, L; Beavis, A W; Saunderson, J R


    The purpose of this study was to examine the use of three physical image quality metrics in the calibration of an automatic exposure control (AEC) device for chest radiography with a computed radiography (CR) imaging system. The metrics assessed were signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR) and mean effective noise equivalent quanta (eNEQm), all measured using a uniform chest phantom. Subsequent calibration curves were derived to ensure each metric was held constant across the tube voltage range. Each curve was assessed for its clinical appropriateness by generating computer simulated chest images with correct detector air kermas for each tube voltage, and grading these against reference images which were reconstructed at detector air kermas correct for the constant detector dose indicator (DDI) curve currently programmed into the AEC device. All simulated chest images contained clinically realistic projected anatomy and anatomical noise and were scored by experienced image evaluators. Constant DDI and CNR curves do not appear to provide optimized performance across the diagnostic energy range. Conversely, constant eNEQm and SNR do appear to provide optimized performance, with the latter being the preferred calibration metric given as it is easier to measure in practice. Medical physicists may use the SNR image quality metric described here when setting up and optimizing AEC devices for chest radiography CR systems with a degree of confidence that resulting clinical image quality will be adequate for the required clinical task. However, this must be done with close cooperation of expert image evaluators, to ensure appropriate levels of detector air kerma.

  6. Comparison of function approximation, heuristic, and derivative-based methods for automatic calibration of computationally expensive groundwater bioremediation models (United States)

    Mugunthan, Pradeep; Shoemaker, Christine A.; Regis, Rommel G.


    The performance of function approximation (FA) methods is compared to heuristic and derivative-based nonlinear optimization methods for automatic calibration of biokinetic parameters of a groundwater bioremediation model of chlorinated ethenes on a hypothetical and a real field case. For the hypothetical case, on the basis of 10 trials on two different objective functions, the FA methods had the lowest mean and smaller deviation of the objective function among all algorithms for a combined Nash-Sutcliffe objective and among all but the derivative-based algorithm for a total squared error objective. The best algorithms in the hypothetical case were applied to calibrate eight parameters to data obtained from a site in California. In three trials the FA methods outperformed heuristic and derivative-based methods for both objective functions. This study indicates that function approximation methods could be a more efficient alternative to heuristic and derivative-based methods for automatic calibration of computationally expensive bioremediation models.

  7. Calibration Method for Formaldehyde Gas Detector Based on Chemical Colorimetric Principle%化学比色原理的甲醛气体检测仪校准方法

    Institute of Scientific and Technical Information of China (English)

    顾玲玲; 舒晓莲; 杨伟浩; 田玉平; 于志强


    A method was put forward for verification of formaldehyde gas detector based on chemical colorimetric principle. JJG–2007 was not suitable for verification of formaldehyde gas detector based on chemical colorimetric principle as the unstability on absorption efficiency of bubble absorption tube and formaldehyde gas solubility being affected by the standard gas partial pressure and temperature. A method was established to calibrate drift, sensitivity, linear error, repeatability and stability of the value with formaldehyde reference material. The method is accurate and reliable to realize measurement calibration and quantity traceability.%提出化学比色原理甲醛气体检测仪的一种校准方法。因为气泡吸收管的吸收效率不恒定及甲醛气体的溶解度受标准气体分压及温度影响,JJG 1022–2007不适用于检定化学比色原理甲醛气体检测仪。建立了以水中甲醛标准物质校准仪器零点漂移、灵敏度、线性误差、示值重复性及稳定性的方法。方法准确可靠,可实现计量校准和量值溯源。

  8. 容积法体积管自动检定装置%Volumetric method volume tube automatic calibration device

    Institute of Scientific and Technical Information of China (English)



    Using standard metal gauge, four-way commutator, PC, PLC as the main hardware, Kingview software development platform, The building Volumetric method volume tube automatic calibration device. According to the verification regulation requirements, To control the flow valve, Device automatically read standard gauge of metal volume value, The automatic temperature and pressure values collected, Automatically calculate the volume tube basic volume, standard error, repeatability, accuracy and repeatability. Realize the visual operation interface, save the test data, print calibration certificate, the notice of verification results, etc.%利用标准金属量器、四通换向器、计算机、PLC 为主要硬件,组态王软件为开发平台,构建容积法体积管自动检定装置。根据检定规程要求,控制流量阀,装置自动读取标准金属量器的容积值、自动采集温度、压力值,自动计算出体积管基本体积、标准误差、重复性、准确度、复现性。实现可视的操作界面、保存检定数据、打印检定证书、检定结果通知书等功能。

  9. Automatic In Situ Calibration of a Spinning Beam LiDAR System in Static and Kinematic Modes

    Directory of Open Access Journals (Sweden)

    Ting On Chan


    Full Text Available The Velodyne LiDAR series is one of the most popular spinning beam LiDAR systems currently available on the market. In this paper, the temporal stability of the range measurements of the Velodyne HDL-32E LiDAR system is first investigated as motivation for the development of a new automatic calibration method that allows quick and frequent recovery of the inherent time-varying errors. The basic principle of the method is that the LiDAR’s internal systematic error parameters are estimated by constraining point clouds of some known and automatically detected cylindrical features such as lamp poles to fit to the 3D cylinder models. This is analogous to the plumb-line calibration method in which the lens distortion parameters are estimated by constraining the image points of straight lines to fit to the 2D line model. The calibration can be performed at every measurement epoch in both static and kinematic modes. Four real datasets were used to verify the method, two of which were captured in static mode and the other two in kinematic mode. The overall results indicate that up to approximately 72% and 41% accuracy improvement were realized as a result of the calibration for the static and kinematic datasets, respectively.

  10. Automatic calibration of a parsimonious ecohydrological model in a sparse basin using the spatio-temporal variation of the NDVI (United States)

    Ruiz-Pérez, Guiomar; Manfreda, Salvatore; Caylor, Kelly; Francés, Félix


    Drylands are extensive, covering 30% of the Earth's land surface and 50% of Africa. In these water-controlled areas, vegetation plays a key role in the water cycle. Ecohydrological models provide a tool to investigate the relationships between vegetation and water resources. However, studies in Africa often face the problem that many ecohydrological models have quite extensive parametrical requirements, while available data are scarce. Therefore, there is a need for searching new sources of information such as satellite data. The advantages of the use of satellite data in dry regions has been deeply demonstrated and studied. But, the use of this kind of data forces to introduce the concept of spatio-temporal information. In this context, we have to deal with the fact that there is a lack in terms of statistics and methodologies to incorporate the spatio-temporal data during the calibration and validation processes. This research wants to be a contribution in that sense. The used ecohydrological model was calibrated in the Upper Ewaso river basin in Kenya only using NDVI (Normalized Difference Vegetation Index) data from MODIS. An automatic calibration methodology based on Singular Value Decomposition techniques was proposed in order to calibrate the model taking into account the temporal variation and, also, the spatial pattern of the observed NDVI and the simulated LAI. The obtained results have demonstrated: (1) the satellite data is an extraordinary useful tool of information and it can be used to implement ecohydrological models in dry regions; (2) the proposed model calibrated only using satellite data is able to reproduce the vegetation dynamics (in time and in space) and, also, the observed discharge at the outlet point; and (3) the proposed automatic calibration methodology works satisfactorily and it includes spatio-temporal data, in other words, it takes into account the temporal variation and the spatial pattern of the analyzed data.

  11. Automatic Calibration Tool for Hydrologic Simulation Program-FORTRAN Using a Shuffled Complex Evolution Algorithm

    Directory of Open Access Journals (Sweden)

    Chounghyun Seong


    Full Text Available Hydrologic Simulation Program-Fortran (HSPF model calibration is typically done manually due to the lack of an automated calibration tool as well as the difficulty of balancing objective functions to be considered. This paper discusses the development and demonstration of an automated calibration tool for HSPF (HSPF-SCE. HSPF-SCE was developed using the open source software “R”. The tool employs the Shuffled Complex Evolution optimization algorithm (SCE-UA to produce a pool of qualified calibration parameter sets from which the modeler chooses a single set of calibrated parameters. Six calibration criteria specified in the Expert System for the Calibration of HSPF (HSPEXP decision support tool were combined to develop a single, composite objective function for HSPF-SCE. The HSPF-SCE tool was demonstrated, and automated and manually calibrated model performance were compared using three Virginia watersheds, where HSPF models had been previously prepared for bacteria total daily maximum load (TMDL development. The example applications demonstrate that HSPF-SCE can be an effective tool for calibrating HSPF.

  12. Towards SWOT data assimilation for hydrology : automatic calibration of global flow routing model parameters in the Amazon basin (United States)

    Mouffe, M.; Getirana, A.; Ricci, S. M.; Lion, C.; Biancamaria, S.; Boone, A.; Mognard, N. M.; Rogel, P.


    The Surface Water and Ocean Topography (SWOT) mission is a swath mapping radar interferometer that will provide global measurements of water surface elevation (WSE). The revisit time depends upon latitude and varies from two (low latitudes) to ten (high latitudes) per 22-day orbit repeat period. The high resolution and the global coverage of the SWOT data open the way for new hydrology studies. Here, the aim is to investigate the use of virtually generated SWOT data to improve discharge simulation using data assimilation techniques. In the framework of the SWOT virtual mission (VM), this study presents the first results of the automatic calibration of a global flow routing (GFR) scheme using SWOT VM measurements for the Amazon basin. The Hydrological Modeling and Analysis Platform (HyMAP) is used along with the MOCOM-UA multi-criteria global optimization algorithm. HyMAP has a 0.25-degree spatial resolution and runs at the daily time step to simulate discharge, water levels and floodplains. The surface runoff and baseflow drainage derived from the Interactions Sol-Biosphère-Atmosphère (ISBA) model are used as inputs for HyMAP. Previous works showed that the use of ENVISAT data enables the reduction of the uncertainty on some of the hydrological model parameters, such as river width and depth, Manning roughness coefficient and groundwater time delay. In the framework of the SWOT preparation work, the automatic calibration procedure was applied using SWOT VM measurements. For this Observing System Experiment (OSE), the synthetical data were obtained applying an instrument simulator (representing realistic SWOT errors) for one hydrological year to HYMAP simulated WSE using a "true" set of parameters. Only pixels representing rivers larger than 100 meters within the Amazon basin are considered to produce SWOT VM measurements. The automatic calibration procedure leads to the estimation of optimal parametersminimizing objective functions that formulate the difference

  13. Automatic exposure control calibration and optimisation for abdomen, pelvis and lumbar spine imaging with an Agfa computed radiography system (United States)

    Moore, C. S.; Wood, T. J.; Avery, G.; Balcam, S.; Needler, L.; Joshi, H.; Saunderson, J. R.; Beavis, A. W.


    The use of three physical image quality metrics, signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR) and mean effective noise equivalent quanta (eNEQm) have recently been examined by our group for their appropriateness in the calibration of an automatic exposure control (AEC) device for chest radiography with an Agfa computed radiography (CR) imaging system. This study uses the same methodology but investigates AEC calibration for abdomen, pelvis and spine CR imaging. AEC calibration curves were derived using a simple uniform phantom (equivalent to 20 cm water) to ensure each metric was held constant across the tube voltage range. Each curve was assessed for its clinical appropriateness by generating computer simulated abdomen, pelvis and spine images (created from real patient CT datasets) with appropriate detector air kermas for each tube voltage, and grading these against reference images which were reconstructed at detector air kermas correct for the constant detector dose indicator (DDI) curve currently programmed into the AEC device. All simulated images contained clinically realistic projected anatomy and were scored by experienced image evaluators. Constant DDI and CNR curves did not provide optimized performance but constant eNEQm and SNR did, with the latter being the preferred calibration metric given that it is easier to measure in practice. This result was consistent with the previous investigation for chest imaging with AEC devices. Medical physicists may therefore use a simple and easily accessible uniform water equivalent phantom to measure the SNR image quality metric described here when calibrating AEC devices for abdomen, pelvis and spine imaging with Agfa CR systems, in the confidence that clinical image quality will be sufficient for the required clinical task. However, to ensure appropriate levels of detector air kerma the advice of expert image evaluators must be sought.

  14. Development of Web Tools for the automatic Upload of Calibration Data into the CMS Condition Data (United States)

    di Guida, Salvatore; Innocente, Vincenzo; Pierro, Antonio


    This article explains the recent evolution of Condition Database Application Service. The Condition Database Application Service is part of the condition database system of the CMS experiment, and it is used for handling and monitoring the CMS detector condition data, and the corresponding computing resources like Oracle Databases, storage service and network devices. We deployed a service, the offline Dropbox service, that will be used by Alignment and Calibration Group in order to upload from the offline network (GPN) the calibration constants produced by running offline analysis.

  15. Automatic calibration of urban drainage model using a novel multi-objective genetic algorithm. (United States)

    di Pierro, F; Djordjević, S; Kapelan, Z; Khu, S T; Savić, D; Walters, G A


    In order to successfully calibrate an urban drainage model, multiple calibration criteria should be considered. This raises the issue of adopting a method for comparing different solutions (parameter sets) according to a set of objectives. Amongst the global optimization techniques that have blossomed in recent years, Multi Objective Genetic Algorithms (MOGA) have proved effective in numerous engineering applications, including sewer network modelling. Most of the techniques rely on the condition of Pareto efficiency to compare different solutions. However, as the number of criteria increases, the ratio of Pareto optimal to feasible solutions increases as well. The pitfalls are twofold: the efficiency of the genetic algorithm search worsens and decision makers are presented with an overwhelming number of equally optimal solutions. This paper proposes a new MOGA, the Preference Ordering Genetic Algorithm, which alleviates the drawbacks of conventional Pareto-based methods. The efficacy of the algorithm is demonstrated on the calibration of a physically-based, distributed sewer network model and the results are compared with those obtained by NSGA-II, a widely used MOGA.

  16. Colorimetric protein assay techniques. (United States)

    Sapan, C V; Lundblad, R L; Price, N C


    There has been an increase in the number of colorimetric assay techniques for the determination of protein concentration over the past 20 years. This has resulted in a perceived increase in sensitivity and accuracy with the advent of new techniques. The present review considers these advances with emphasis on the potential use of such technologies in the assay of biopharmaceuticals. The techniques reviewed include Coomassie Blue G-250 dye binding (the Bradford assay), the Lowry assay, the bicinchoninic acid assay and the biuret assay. It is shown that each assay has advantages and disadvantages relative to sensitivity, ease of performance, acceptance in the literature, accuracy and reproducibility/coefficient of variation/laboratory-to-laboratory variation. A comparison of the use of several assays with the same sample population is presented. It is suggested that the most critical issue in the use of a chromogenic protein assay for the characterization of a biopharmaceutical is the selection of a standard for the calibration of the assay; it is crucial that the standard be representative of the sample. If it is not possible to match the standard with the sample from the perspective of protein composition, then it is preferable to use an assay that is not sensitive to the composition of the protein such as a micro-Kjeldahl technique, quantitative amino acid analysis or the biuret assay. In a complex mixture it might be inappropriate to focus on a general method of protein determination and much more informative to use specific methods relating to the protein(s) of particular interest, using either specific assays or antibody-based methods. The key point is that whatever method is adopted as the 'gold standard' for a given protein, this method needs to be used routinely for calibration.

  17. A versatile and reproducible automatic injection system for liquid standard introduction: application to in-situ calibration

    Directory of Open Access Journals (Sweden)

    G. Isaacman


    Full Text Available The quantitation of trace organic compounds in ambient organic aerosol is difficult due to the chemical complexity of these mixtures, but is needed to provide insight into their sources and formation processes. Compound-level characterization of organic aerosols is typically performed through sample collection followed by gas or liquid chromatography. With these methods, introduction of liquid standards has long been used as an effective means of quantifying trace compounds, but automating this technique for use with in-situ instrumentation has not previously been achieved. Here we develop an automatic injection system (AutoInject for the introduction of liquids into a custom collection and analysis cell for improved quantitation in chromatographic measurements. The system consists of chilled reservoirs containing liquid standards from which a sample loop is loaded and then injected into the cell. The AutoInject is shown to have reproducibility over 106 injections with a relative standard deviation of 1.5 %, and have negligible injection-to-injection carryover. A 6-port selector allows injection of different liquid standards separately or simultaneously. Additionally, automatic injection of multiple sample loops is shown to generate a linear multi-point calibration curve. Tests conducted in this work focus on use with the Thermal desorption Aerosol Gas chromatograph (TAG, but the flexibility of the system allows it to be used for a variety of applications.

  18. Automatic high-bandwidth calibration and reconstruction of arbitrarily sampled parallel MRI.

    Directory of Open Access Journals (Sweden)

    Jan Aelterman

    Full Text Available Today, many MRI reconstruction techniques exist for undersampled MRI data. Regularization-based techniques inspired by compressed sensing allow for the reconstruction of undersampled data that would lead to an ill-posed reconstruction problem. Parallel imaging enables the reconstruction of MRI images from undersampled multi-coil data that leads to a well-posed reconstruction problem. Autocalibrating pMRI techniques encompass pMRI techniques where no explicit knowledge of the coil sensivities is required. A first purpose of this paper is to derive a novel autocalibration approach for pMRI that allows for the estimation and use of smooth, but high-bandwidth coil profiles instead of a compactly supported kernel. These high-bandwidth models adhere more accurately to the physics of an antenna system. The second purpose of this paper is to demonstrate the feasibility of a parameter-free reconstruction algorithm that combines autocalibrating pMRI and compressed sensing. Therefore, we present several techniques for automatic parameter estimation in MRI reconstruction. Experiments show that a higher reconstruction accuracy can be had using high-bandwidth coil models and that the automatic parameter choices yield an acceptable result.

  19. Application of DFSS in Automatic Transmission Calibration%DFSS设计方法在自动变速器标定中的应用

    Institute of Scientific and Technical Information of China (English)

    闫勋成; 何宁; 冀海燕; 田华


    This paper, based on DFSS design method theory and the basic procedure of automatic transmission shift quality calibration, takes the 2-4 speed upshift quality as an example for a vehicle model equipped with automatic transmission when the acceleration pedal is released, to expound the application of DFSS in automatic transmission calibration, and this design method is applied in the main parameters of automatic transmission calibration for optimization design. The results show that, with this design method, the automatic transmission shift quality robustness is improved.%基于DFSS设计方法理论和自动变速器换挡质量标定的基本流程,以某匹配6挡自动变速器的车型松油门时2挡升4挡换挡质量问题为例,阐述了DFSS设计方法在自动变速器标定开发过程中的应用,并应用该设计方法对自动变速器标定中的主要参数进行优化设计.结果表明,应用该设计方法后自动变速器换挡质量的稳健性得到提高.

  20. Multiplexed Colorimetric Solid-Phase Extraction (United States)

    Gazda, Daniel B.; Fritz, James S.; Porter, Marc D.


    Multiplexed colorimetric solid-phase extraction (MC-SPE) is an extension of colorimetric solid-phase extraction (C-SPE) an analytical platform that combines colorimetric reagents, solid phase extraction, and diffuse reflectance spectroscopy to quantify trace analytes in water. In CSPE, analytes are extracted and complexed on the surface of an extraction membrane impregnated with a colorimetric reagent. The analytes are then quantified directly on the membrane surface using a handheld diffuse reflectance spectrophotometer. Importantly, the use of solid-phase extraction membranes as the matrix for impregnation of the colorimetric reagents creates a concentration factor that enables the detection of low concentrations of analytes in small sample volumes. In extending C-SPE to a multiplexed format, a filter holder that incorporates discrete analysis channels and a jig that facilitates the concurrent operation of multiple sample syringes have been designed, enabling the simultaneous determination of multiple analytes. Separate, single analyte membranes, placed in a readout cartridge create unique, analyte-specific addresses at the exit of each channel. Following sample exposure, the diffuse reflectance spectrum of each address is collected serially and the Kubelka-Munk function is used to quantify each water quality parameter via calibration curves. In a demonstration, MC-SPE was used to measure the pH of a sample and quantitate Ag(I) and Ni(II).

  1. Automatic calibration of an erosion and sediment yield distributed conceptual model: application to the Goodwin Creek experimental river basin (USA) (United States)

    Bussi, G.; Francés, F.


    In the last decades, distributed hydrological models have achieved a fundamental importance in Hydrology, mainly for their capacity to describe the spatial variability of the basin processes. TETIS is a distributed conceptual model created to simulate rainfall-runoff processes. In the same way, a distributed approach to erosion and sediment yield modelling can lead to improvements for the solution of several sedimentological and geomorphological problems, such as sediment redistribution, localization of heavy erosion and soil loss zones, estimation of soil erosion and sediment yield and assessment of land use change effects on the sediment cycle. Following these considerations, the TETIS model has been coupled with a sediment cycle module with the purpose of representing erosion and sediment transport at basin scale. TETIS-SED is the result of integrating the erosion submodel of CASC2D-SED into the hydrological model TETIS. In the TETIS-SED model, the erosion/sedimentation rates are calculated as a function of the hydraulic properties of the flow, the physical properties of the soil and the surface characteristics. The modified Kilinc-Richardson equation is used to determine the upland sediment transport by grain size (silt, clay, and sand) from one cell into the next one. Sediment by size fraction is routed in the channels and the Engelund and Hansen equation is used to compute the transport capacity in one dimension. This formulation in both cases depends on hydraulic parameters (hydraulic radius, flow velocity and friction slope) and particle characteristics (specific gravity and particle diameter). Due to the uncertainty affecting the sediment parameters, the calibration stage may be a key issue in erosion and sediment yield modelling. In the TETIS model, automatic calibration is carried out by adjusting up to 9 hydrological correction factors with an automatic calibration algorithm, the Shuffled Complex Evolution (SCE-UA). In this work, 3 sedimentological

  2. A versatile photogrammetric camera automatic calibration suite for multispectral fusion and optical helmet tracking (United States)

    de Villiers, Jason; Jermy, Robert; Nicolls, Fred


    This paper presents a system to determine the photogrammetric parameters of a camera. The lens distortion, focal length and camera six degree of freedom (DOF) position are calculated. The system caters for cameras of different sensitivity spectra and fields of view without any mechanical modifications. The distortion characterization, a variant of Brown's classic plumb line method, allows many radial and tangential distortion coefficients and finds the optimal principal point. Typical values are 5 radial and 3 tangential coefficients. These parameters are determined stably and demonstrably produce superior results to low order models despite popular and prevalent misconceptions to the contrary. The system produces coefficients to model both the distorted to undistorted pixel coordinate transformation (e.g. for target designation) and the inverse transformation (e.g. for image stitching and fusion) allowing deterministic rates far exceeding real time. The focal length is determined to minimise the error in absolute photogrammetric positional measurement for both multi camera systems or monocular (e.g. helmet tracker) systems. The system determines the 6 DOF position of the camera in a chosen coordinate system. It can also determine the 6 DOF offset of the camera relative to its mechanical mount. This allows faulty cameras to be replaced without requiring a recalibration of the entire system (such as an aircraft cockpit). Results from two simple applications of the calibration results are presented: stitching and fusion of the images from a dual-band visual/ LWIR camera array, and a simple laboratory optical helmet tracker.

  3. Automatic Calibration of a Distributed Rainfall-Runoff Model, Using the Degree-Day Formulation for Snow Melting, Within DMIP2 Project (United States)

    Frances, F.; Orozco, I.


    This work presents the assessment of the TETIS distributed hydrological model in mountain basins of the American and Carson rivers in Sierra Nevada (USA) at hourly time discretization, as part of the DMIP2 Project. In TETIS each cell of the spatial grid conceptualizes the water cycle using six tanks connected among them. The relationship between tanks depends on the case, although at the end in most situations, simple linear reservoirs and flow thresholds schemes are used with exceptional results (Vélez et al., 1999; Francés et al., 2002). In particular, within the snow tank, snow melting is based in this work on the simple degree-day method with spatial constant parameters. The TETIS model includes an automatic calibration module, based on the SCE-UA algorithm (Duan et al., 1992; Duan et al., 1994) and the model effective parameters are organized following a split structure, as presented by Francés and Benito (1995) and Francés et al. (2007). In this way, the calibration involves in TETIS up to 9 correction factors (CFs), which correct globally the different parameter maps instead of each parameter cell value, thus reducing drastically the number of variables to be calibrated. This strategy allows for a fast and agile modification in different hydrological processes preserving the spatial structure of each parameter map. With the snowmelt submodel, automatic model calibration was carried out in three steps, separating the calibration of rainfall-runoff and snowmelt parameters. In the first step, the automatic calibration of the CFs during the period 05/20/1990 to 07/31/1990 in the American River (without snow influence), gave a Nash-Sutcliffe Efficiency (NSE) index of 0.92. The calibration of the three degree-day parameters was done using all the SNOTEL stations in the American and Carson rivers. Finally, using previous calibrations as initial values, the complete calibration done in the Carson River for the period 10/01/1992 to 07/31/1993 gave a NSE index of

  4. [A new colorimetric CO2 indicator Colibri]. (United States)

    Nishiyama, T; Hanaoka, K


    A new colorimetric carbon dioxide (CO2) indicator Colibri is a disposable, compact and light weighted device. Colibri does not need to be calibrated and is easily usable in an emergency. It indicates blue with CO2 below 4 mmHg and becomes yellow with CO2 above 40 mmHg. In comparison with EASY CAP, Colibri functions for a longer period and it has a humidifier and a bacterial filter. Colibri is useful for emergency situations and anesthetic care.

  5. Colorimetric Determination of Hydroxylurea

    Institute of Scientific and Technical Information of China (English)

    ZHANGYu; ZHUZhao-wu


    Hydroxyurea is able to form colored complex with amminoprusside (TPF) which has strong absorption at 500 nm suitable for the colorimetric determination of hydroxyurea. Present paper studies the effects of temperature, quantity of buffer solution, quantity of TPF used and so on, on the complex absorption.

  6. FlowCal: A User-Friendly, Open Source Software Tool for Automatically Converting Flow Cytometry Data from Arbitrary to Calibrated Units. (United States)

    Castillo-Hair, Sebastian M; Sexton, John T; Landry, Brian P; Olson, Evan J; Igoshin, Oleg A; Tabor, Jeffrey J


    Flow cytometry is widely used to measure gene expression and other molecular biological processes with single cell resolution via fluorescent probes. Flow cytometers output data in arbitrary units (a.u.) that vary with the probe, instrument, and settings. Arbitrary units can be converted to the calibrated unit molecules of equivalent fluorophore (MEF) using commercially available calibration particles. However, there is no convenient, nonproprietary tool available to perform this calibration. Consequently, most researchers report data in a.u., limiting interpretation. Here, we report a software tool named FlowCal to overcome current limitations. FlowCal can be run using an intuitive Microsoft Excel interface, or customizable Python scripts. The software accepts Flow Cytometry Standard (FCS) files as inputs and is compatible with different calibration particles, fluorescent probes, and cell types. Additionally, FlowCal automatically gates data, calculates common statistics, and produces publication quality plots. We validate FlowCal by calibrating a.u. measurements of E. coli expressing superfolder GFP (sfGFP) collected at 10 different detector sensitivity (gain) settings to a single MEF value. Additionally, we reduce day-to-day variability in replicate E. coli sfGFP expression measurements due to instrument drift by 33%, and calibrate S. cerevisiae Venus expression data to MEF units. Finally, we demonstrate a simple method for using FlowCal to calibrate fluorescence units across different cytometers. FlowCal should ease the quantitative analysis of flow cytometry data within and across laboratories and facilitate the adoption of standard fluorescence units in synthetic biology and beyond.

  7. 浅谈全自动凯氏定氮仪的的校准及维护%The Calibration Technique and Maintaining for Automatic Kjeldahl Analyzer

    Institute of Scientific and Technical Information of China (English)

    施江焕; 王维峰; 余善成


    旨在统一全自动凯氏定氮仪的校准方法,本文从仪器的工作原理及结构入手,对该类仪器提出了检测限、测量线性、回收率、精密度等性能评价参数,规范了校准方法,分析了仪器常见的故障及排除方法,校准及维护方法科学合理且可操作性强,有力保证了测量数据的准确性、溯源性,对计量检定机构开展校准工作及今后规程的制定提供了参考。%For unifying the calibration method of automatic Kjeldahl analyzer,combining with the working principle and structure of the instrument,this paper proposes performance evaluation parameters including the detection limit,measuring linearity,recovery,precision and standardized calibration method,and analysis common faults and troubleshooting.The calibration and maintenance method is scientifically feasible which could provide some guidance on the measurement of calibration and formulation of verification regulation for Metrological verification institutions to effectively ensure the accuracy and traceability of the measurement data.

  8. Automatic anatomy partitioning of the torso region on CT images by using multiple organ localizations with a group-wise calibration technique (United States)

    Zhou, Xiangrong; Morita, Syoichi; Zhou, Xinxin; Chen, Huayue; Hara, Takeshi; Yokoyama, Ryujiro; Kanematsu, Masayuki; Hoshi, Hiroaki; Fujita, Hiroshi


    This paper describes an automatic approach for anatomy partitioning on three-dimensional (3D) computedtomography (CT) images that divide the human torso into several volume-of-interesting (VOI) images based on anatomical definition. The proposed approach combines several individual detections of organ-location with a groupwise organ-location calibration and correction to achieve an automatic and robust multiple-organ localization task. The essence of the proposed method is to jointly detect the 3D minimum bounding box for each type of organ shown on CT images based on intra-organ-image-textures and inter-organ-spatial-relationship in the anatomy. Machine-learning-based template matching and generalized Hough transform-based point-distribution estimation are used in the detection and calibration processes. We apply this approach to the automatic partitioning of a torso region on CT images, which are divided into 35 VOIs presenting major organ regions and tissues required by routine diagnosis in clinical medicine. A database containing 4,300 patient cases of high-resolution 3D torso CT images is used for training and performance evaluations. We confirmed that the proposed method was successful in target organ localization on more than 95% of CT cases. Only two organs (gallbladder and pancreas) showed a lower success rate: 71 and 78% respectively. In addition, we applied this approach to another database that included 287 patient cases of whole-body CT images scanned for positron emission tomography (PET) studies and used for additional performance evaluation. The experimental results showed that no significant difference between the anatomy partitioning results from those two databases except regarding the spleen. All experimental results showed that the proposed approach was efficient and useful in accomplishing localization tasks for major organs and tissues on CT images scanned using different protocols.

  9. Monitoring of the infrastructure and services used to handle and automatically produce Alignment and Calibration conditions at CMS

    CERN Document Server

    Sipos, Roland


    The Compact Muon Solenoid (CMS) experiment makes a vast use of alignment and calibration measurements in several crucial workflows in the event selection at the High Level Trigger (HLT), in the processing of the recorded collisions and in the production of simulated events.A suite of services addresses the key requirements for the handling of the alignment and calibration conditions such as recording the status of the experiment and of the ongoing data taking, accepting conditions data updates provided by the detector experts, aggregating and navigating the calibration scenarios, and distributing conditions for consumption by the collaborators. Since a large fraction of such services is critical for the data taking and event filtering in the HLT, a comprehensive monitoring and alarm generating system had to be developed. Such monitoring system has been developed based on the open source industry standard for monitoring and alerting services (Nagios) to monitor the database back-end, the hosting nodes and k...

  10. Research of automatic control system for uitralow frequency vibration calibration%超低频振动校准自动控制系统的研究

    Institute of Scientific and Technical Information of China (English)

    王志鹏; 何闻; 于梅; 沈润杰


    针对超低频振动校准过程中测试时间长且效率较低的问题,设计了一种基于个人计算机及虚拟仪器技术开发的超低频振动校准自动控制系统.该系统由超低频标准振动台、功率放大器、激光测振仪、位移传感器、信号发生器、频比计数器、数据采集卡、计算机与测控软件等组成.通过线性逼近与逐步移频相结合的算法,精确控制振动台在超低频时迅速到达设定振级.试验结果证明该系统满足超低频振动校准要求,提高了超低频振动传感器的测试精度和自动化程度.%Aiming at the demerits of time-consuming and low efficiency during the process of ultralow frequency vibration calibration, an automatic ultralow frequency vibration calibration system based on personal computer and virtual instrument was presented.The system was consisted of ultralow frequency standard vibrator, power amplifier, laser vibrometer, displacement transducer, signal generator, universal counter, data acquisition card, computer, control software, etc..The strategy which via combining the method of linear approximation and stepby-step frequency shift could make the vibrator reach the pre-set vibration level quickly and precisely.The experiment results show that the system is suitable for the calibrating ultralow frequency vibration transducers very well and improves the automation of ultralow frequency calibrating vibration.

  11. 电子数显量表的全自动检定仪设计%Design of automatic calibrator for electronic digital scale meter

    Institute of Scientific and Technical Information of China (English)

    吴佳雄; 吴佳伟; 李自立


    A computer automatic calibrator system for capacitive gate-controlled electronic digital scale meter is proposed. Delphi PC control software is usd, and RS232 is used for data transmission. By setting the appropriate parameters and controlling the stepper motor instead of manually rotating fixtures,all the real-time value of checkpoints and grating digital table is got. It has been achieved fully automatic detection and data processing for the electronic scale meter. Due to eliminating the human factors in calibration process,it can be not only improve the test speed but also the test accuracy. Therefore the system has high reliability,accuracy,test speed and is easy to operate.%给出了一种采用计算机控制的全自动容栅电子数显量表检定系统.系统设计了Delphi上位机控制软件,采用RS232接口进行数据传输.通过设置相应参数,控制步进电机代替人工转动卡具,读取数显量表的各个被检点和光栅表的实时数值,实现计算机对容栅电子数显量表的自动检测和数据处理.系统操作方便、可靠性好、准确性高,测量速度快,消除了检定过程中的人为因素,既提高了检定速度,又提高了检定精度.

  12. Colorimetric Scanner Characterisation

    Directory of Open Access Journals (Sweden)

    Jon Y. Hardeberg


    Full Text Available In this paper, methods for the colorimetric characterisation of colour scanners are proposed and evaluated. These methods apply equally to other colour image input devices such as digital cameras. The goal of our characterisation is to establish the relationship between the device-dependent colour space of the scanner and the device-independent CIELAB colour space. The scanner characterisation is based on polynomial regression techniques. Several regression schemes have been tested. The retained method consists in applying a non-linear correction to the scanner RGB values followed by a 3rd order 3D polynomial regression function directly to CIELAB space. This method gives very good results in terms of residual colour differences. This is partly due to the fact that the RMS error that is minimised in the regression corresponds to ΔE*ab which is well correlated to visual colour differences.

  13. 指针式压力表自动检定系统的设计与实现%Design and implementation of fully automatic calibration system for pressure gauges with pointers

    Institute of Scientific and Technical Information of China (English)

    熊伟丽; 周寰; 徐保国


    A fully automatic calibration system for pressure gauges with pointers is presented, including a calibration management system of pressure gauges and an automatic reading system of pointer readings. The operation is very convenient with the use of visual interface in this system. The operator only needs to input several necessary coefficients such as range, accuracy and so on, the pointer reading of pressure gauges will be read automatically. The automatic reading method will be used to calibrate for almost all kinds of pointer gauges. The accuracy is decided by resolution and processing time. This automatic calibration system improves the present state of pressure calibration, and enhances the automation level of calibration to some extent.%设计并开发了一种指针式压力表自动检定系统.该系统包括压力表检定/校准管理系统和仪表示值自动判读系统两部分.系统的可视化界面使得操作非常简单,操作人员只需输入量程、精度等必要参数,压力表的指针读数即可自动读出.给出的自动判读方法可用于各种指针式仪表精度的自动检测、判读,其精度取决于图像的分辨率和限定的处理时间.本系统可较大地改善压力表人工检定工作的落后状况,在一定程度上提高了检定工作的自动化水平和检定结果的稳定性.

  14. The Implement of Oscilloscope Calibrator Automatically Verification System%示波器校准仪自动计量检定系统的实现

    Institute of Scientific and Technical Information of China (English)

    张文娟; 马春雷; 吕燕


    Oscilloscope as one of the commonly used measuring and testing equipment, has been widely used in the production and research activities. However, the data accuracy, reliability and convenience of operation shall be the most features of Oscilloscope calibrator, as the standardization equipment. In order to ensure the stable performance, and accuracy of the measurement, route performance evaluation is quite necessary. This thesis defined the testing system of oscilloscope calibrator based on GPIB bus architecture from the perspective of computer technology, software technology and measuring technology of automatic testing system.%示波器是最常用的测量与测试工具,在科研和生产中被大量使用.而示波器校准仪作为其检定标准装置,其量值的准确、可靠以及检定的方便、快捷显得尤为重要.为确保示波器校准仪的性能稳定及测量的准确性,需要定期对示波器校准仪进行检定.本论文从自动化测试系统中的计算机技术、软件技术和测量技术人手,确定了基于GPIB总线体系架构的示波器校准仪自动计量检定系统.

  15. Calibration Method of Nickel Water Quality On-line Automatic Monitoring Instrument%镍水质在线自动监测仪校准方法

    Institute of Scientific and Technical Information of China (English)



    通过分析镍水质在线自动监测仪工作原理及结构,探讨其计量特性和校准方法。提出了校准项目和技术指标,示值误差:当镍的质量浓度c≤0.05 mg/L时为±0.01 mg/L,当0.05 mg/L<c≤0.2 mg/L时为±10%,当c>0.2 mg/L时为±5%;重复性不大于5%;稳定性不大于5%。对示值误差测量结果不确定度进行了评定,相对扩展不确定度为1.6%(k=2)。用所建校准方法对不同厂家的监测仪进行校准试验,示值误差、重复性、稳定性满足校准项目技术指标要求。该校准方法可以用于评价镍水质在线自动监测仪的性能。%The metrological characteristics and calibration method of the nickel water quality on-line automatic monitoring instrument were discussed on the basis of analysis of its working principle and structure. The calibration items and technical specifications were put forward. The indicating value error were±0.01 mg/L,±10% and±5% at the corresponding concentration of standard solutionc≤0.05 mg/L,0.05 mg/L0.2 mg/L, respectively. The repeatability was not more than 5% while the stability was not more than 5%. The measurement uncertainty of indicating value error was evaluated,and the relative expanded uncertainty was 1.6% (k=2). The established calibration method was applied to test monitoring instruments from different manufacturers. The results indicated that value error, repeatability and stability meet the requirement of technical specifications. This method can be used for the evaluation of the performance of nickel water quality on-line automatic monitoring instrument.

  16. Design and implementation of automatic color information collection system (United States)

    Ci, Wenjie; Xie, Kai; Li, Tong


    In liquid crystal display (LCD) colorimetric characterization, it needs to convert RGB the device-dependent color space to CIEXYZ or CIELab the device-independent color space. Namely establishing the relationship between RGB and CIE using the data of device color and the corresponding data of CIE. Thus a color automatic message acquisition software is designed. We use openGL to fulfill the full screen display function, write c++ program and call the Eyeone equipment library functions to accomplish the equipment calibration, set the sample types, and realize functions such as sampling and preservation. The software can drive monitors or projectors display the set of sample colors automatically and collect the corresponding CIE values. The sample color of RGB values and the acquisition of CIE values can be stored in a text document, which is convenient for future extraction and analysis. Taking the cubic polynomial as an example, each channel is sampled of 17 sets using this system. And 100 sets of test data are also sampled. Using the least square method we can get the model. The average of color differences are around 2.4874, which is much lower than the CIE2000 commonly required level of 6.00.The successful implementation of the system saves the time of sample color data acquisition, and improves the efficiency of LCD colorimetric characterization.

  17. 某高炮武器系统自动校射系统设计%Design of Automatic Firing Calibration System of a Certain Antiaircraft Artillery Weapon System

    Institute of Scientific and Technical Information of China (English)

    马志强; 程远增; 孙世宇; 王春平


    我国现役高炮火控系统的校射大多采用开环控制,没有自动校射功能,作战效能较低.针对某型高炮设计了一种自动校射系统,该系统采用火控解算参数与数字图像处理技术相结合的方法,火控计算机计算出虚拟弹目偏差,图像处理技术获取置信度较高的弹目偏差,对高炮射击诸元进行实时校正,克服传统校射的不足,从而实现对高炮武器系统的全过程高精度自动校射,实现高炮武器系统的快速射击,提高射击命中率,充分发挥高炮武器系统的作战效能.%China's active antiaircraft artillery fire control system mostly uses open-loop control for fireng calibration, no automatic fire calibration function, only has low operation efficiency. This paper presents a design method of automatic fire calibration system to a certain antiaircraft artillery. Combining fire control system computing parameters with digital image processing technology, automatic fire calibration system uses fire control computer computing the virtual miss distance, uses image processing technology obtaining miss distance with higher reliability in order to correct real-timely all kinds of firing data of antiaircraft artillery and overcome the deficiencies of traditional fire calibration method. For the antiaircraft artillery weapon system, this system could achieve automatic fire calibration with the whole process and high precision, ensure rapid firing and improve hit rate, give full play to the combat effectiveness.

  18. Individual Colorimetric Observer Model.

    Directory of Open Access Journals (Sweden)

    Yuta Asano

    Full Text Available This study proposes a vision model for individual colorimetric observers. The proposed model can be beneficial in many color-critical applications such as color grading and soft proofing to assess ranges of color matches instead of a single average match. We extended the CIE 2006 physiological observer by adding eight additional physiological parameters to model individual color-normal observers. These eight parameters control lens pigment density, macular pigment density, optical densities of L-, M-, and S-cone photopigments, and λmax shifts of L-, M-, and S-cone photopigments. By identifying the variability of each physiological parameter, the model can simulate color matching functions among color-normal populations using Monte Carlo simulation. The variabilities of the eight parameters were identified through two steps. In the first step, extensive reviews of past studies were performed for each of the eight physiological parameters. In the second step, the obtained variabilities were scaled to fit a color matching dataset. The model was validated using three different datasets: traditional color matching, applied color matching, and Rayleigh matches.

  19. 自动生化分析系统校准品的检测性能验证%Performance verification of calibrator for automatic biochemical analysis systems

    Institute of Scientific and Technical Information of China (English)

    张丽; 管晓媛; 段兵; 黄一玲; 田蕾; 李一石


    目的:以乳酸脱氢酶(LDH)为例,验证罗氏新批号自动生化分析系统校准品(Cfas)的检测性能。方法在罗氏Cobas C501全自动生化分析仪上,使用20份新鲜患者血清样本,于Cfas批号更换前后分别检测LDH。采用Bland‐Altman散点图方法,用Medcalc12.7.0统计学软件分析检测结果。结果散点图中,有19个点位于一致性界限(LoA)范围内,超过所有点的95%;LDH两次测定结果差值均数仅为-0.2 U/L ,与差值均数为0的线非常接近;LDH两次测量结果最大差值为8U/L,最大比对偏差为2.48%,小于比对偏差可接受标准(2.87%),这种偏差幅度在临床上可以接受。结论在更换Cfas批号前后LDH的两次检测结果具有一致性,新批号Cfas性能验证通过。%Objective To verify the performance of calibrator with new batch number for automatic biochemi‐cal analysis systems (Cfas), using lactate dehydrogenase (LDH) as an example .Methods LDH of twenty serum samples were detected by Roche Cobas C501 automatic biochemical analyzer before and after replacement of calibrator with new batch number .The detection results were analyzed by Bland‐Altman analysis method and Medcalc 12 .7 .0 software .Results According to the Bland‐Altman scatter diagram, a total of 19 dots were within limits of agreement (LoA), accounting for more than 95% of all dots .The mean value of difference between the results of twice detection was only -0 .2 U/L, which was very closed to zero, and the maximum difference was 8 U/L .The largest comparabil‐ity deviation (2 .48% ) was less than the comparability standard deviation(2 .87% ), which was clinically acceptable . Conclusion The detectable results of LDH before and after replacement of calibrator with new batch number could be in accordance, indicating the performance of the calibrator with new batch numbe might be acceptable .

  20. Automatic Titrator Calibration Standard Solution of Silver Nitrate%自动电位滴定仪标定硝酸银标准溶液

    Institute of Scientific and Technical Information of China (English)



      Using the configuration DM141-SC-type silver electrode DL58 automatic titrator calibration standard solution of silver nitrate, accuracy and precision compared with manual titration with a large degree of increase, and simple operation, equipment maintenance, more accurate titration results .Titration process signal acquisition is 0.1mV, minimum feed rate can be titrated to achieve 0.001ml, is the recommended method.%  采用配置DM141-SC型银电极的DL58型自动电位滴定仪标定硝酸银标准溶液,准确度及精密度均较手动滴定有很大程度的提高,且操作简单,仪器维护方便,滴定结果更准确,滴定过程采集信号为0.1mV,滴定最小进给量可达到0.001mL,是目前值得推荐的方法.

  1. VP宽频带倾斜仪现场自动校准装置设计%Design of an On-site Automatic Calibration Device for the Vertical Pendulum Broadband Tiltmeter

    Institute of Scientific and Technical Information of China (English)

    马武刚; 张肖


    为了对VP宽频带倾斜仪进行现场校准,设计了一款基于单片机 AT89 C51的新型现场自动校准装置。经验证该装置在+8V激励电压情况下与高精度激光干涉仪结合使用,其校准相对误差为0.36%,满足小于1%的校准相对误差要求。%The vertical pendulum (VP)broadband tiltmeter is a type of earthquake precursor observation equipment that records the inclined earth tide.The scale value represents the relation-ship between the measured physical quantity and the output voltage.Its accuracy plays a key role in data reliability.The calibration process provides the scale value for the instrument.Most tradi-tional calibration methods,e.g.,plat calibrations in the laboratory and manual calibrations on site, cannot satisfy the accuracy levels required in scientific applications,or might affect the continuity of observations.In this study,we investigate an on-site automatic calibration device,in which small-scale shifts are introduced by a piezoelectric ceramic under the control of a single chip microcomputer AT89C51.The device then calculates the scale values from serial corresponding values of the outputs of the VP broadband tiltmeter and a laser interferometer.This device could realize the automatic operation of calibration,and its relative calibration accuracy is 0.36% when the excitation voltage is adj usted to +8 V,and meets the requirement of a relative error of less than 1%.

  2. A Colorimetric Interdental Probe as a Standard Method to Evaluate Interdental Efficiency of Interdental Brush. (United States)

    Bourgeois, D; Carrouel, F; Llodra, J C; Bravo, M; Viennot, S


    The aim of this study is to evaluate the concordance between the empirical choice of interdental brushes of different diameters compared to the gold standard, the IAP CURAPROX(©) calibrating colorimetric probe. It is carried out with the aim of facilitating the consensus development of best practices. All the subjects' interproximal spaces were evaluated using the reference technique (colorimetric probe), then after a time lapse of 1.2 ± 0.2 hours, using the empirical clinical technique (brushes) by the same examiner. Each examiner explored 3 subjects. The order the patients were examined with the colorimetric interdental probe (CIP) was random. 446 sites were selected in the study out of 468 potential sites. The correspondence of scores between interdental bushes vs. colorimetric probe is 43.0% [95%-CI: 38.5-47.6]. In 33.41% of the 446 sites, the brush is inferior to the probe; in 23.54% of cases, the brush is superior to the probe. Among the discrepancies there is thus a tendency for the subjects to use brushes with smaller diameter than that recommended by the colorimetric probe. This review has found very high-quality evidence that colorimetric probes plus interdental brushing is more beneficial than interdental brushing alone for increase the concordance between the empirical choice of interdental brushes of different diameters compared to the gold standard. Uncertainties remain and further research is required to provide detailed data on user satisfaction.

  3. Checker pattern improvement and fully-automatic identification for camera calibration%摄像机标定的棋盘格模板的改进和自动识别

    Institute of Scientific and Technical Information of China (English)

    张浩鹏; 王宗义; 吴攀超; 林欣堂


    为了克服在摄像机标定过程中需要使用者给出标定模板的附加信息,或全自动标定点识别算法在遮挡、不均匀照明、大视角和摄像机镜头畸变情况下不能检测出标定点的缺点,提出一种改进的基于基准点标记的棋盘格模板以及相应的全自动识别算法.新的摄像机标定模板以基准点标记代替传统棋盘格的黑白方块,从而使全自动识别算法识别出标记的位置.利用模板中标记按照标记ID从小到大的顺序排列的先验知识,估计丢失的标定点位置.为了提高丢失标定点在图像中初始位置的估计,算法估计径向畸变参数,从而克服了畸变对识别的影响.为了提高标定点的定位精度,利用高精度的鞍点检测器,从而标定点的定位精度小于0.05像素.为了检测鞍点的有效性,算法提出2种滤波准则,最终得到有效的标定点.识别算法是有效的且不需要任何参数.实验结果表明,对于同样的摄像机和背景,使用改进的棋盘格模板及其识别算法获得的标定点进行摄像机标定的投影误差比ARTag减少70%.%In order to overcome the shortcomings that in camera calibration the user needs to give additional information of calibration pattern or fully-automatic identification algorithm of calibration points can not detect calibration points under the conditions of significant occlusions, uneven illumination, observation with extremely viewing angles and lens distortion, an improved checker pattern based on fiducial markers is designed, and the corresponding fully-automatic identification algorithm of the calibration points is proposed. The new camera calibration pattern replaces the black and white squares in traditional checker pattern with the fiducial markers, so the fully-automatic identification algorithm can locate the positions of the markers. Using the priori knowledge that the markers are arranged sequentially in the calibration pattern according to

  4. Study on a New Type of Automatic Loading Device for Strain Gauge Balance Calibration%新型应变天平校准系统自动加载装置研究

    Institute of Scientific and Technical Information of China (English)


    Considering the characteristics of the wind tunnel balance calibration system,the automatic weight loading is the most important part.The clamping modules are the first time to be used as the lifting device in the weight mechanism design for the strain gauge balance calibration system,with them the structure of weight loading mechanism is greatly simplified.%风洞应变天平是风洞测力实验中最关键测量装置。为实现风洞应变天平校准系统的砝码自动加载,通过分析对比现有砝码自动加载方式的优缺点,结合应变天平校准系统特点,设计并研制采用膜片式夹紧气缸作为砝码升降机构执行元件的砝码加载装置。加载装置具有结构简单、体积小巧、性能稳定等优点。

  5. Colorimetric detection of hazardous gases using a remotely operated capturing and processing system. (United States)

    Montes-Robles, Roberto; Moragues, María Esperanza; Vivancos, José-Luis; Ibáñez, Javier; Fraile, Rubén; Martínez-Máñez, Ramón; García-Breijo, Eduardo


    This paper presents an electronic system for the automatic detection of hazardous gases. The proposed system implements colorimetric sensing algorithms, thus providing a low-cost solution to the problem of gas sensing. It is remotely operated and it performs the tasks of image capturing and processing, hence obtaining colour measurements in RGB (Red-Green-Blue) space that are subsequently sent to a remote operator via the internet. A prototype of the system has been built to test its performance. Specifically, experiments have been carried out aimed at the detection of CO, CO2, NO, NO2, SO2 and formaldehyde at diverse concentrations by using a chromogenic array composed by 13 active and 2 inert compounds. Statistical analyses of the results reveal a good performance of the electronic system and the feasibility of remote hazardous gas detection using colorimetric sensor arrays.

  6. A colorimetric assay for cytokinin oxidase. (United States)

    Libreros-Minotta, C A; Tipton, P A


    A simple and rapid colorimetric assay for cytokinin oxidase is described. The assay is based on the formation of a Schiff base between the enzymatic reaction product 3-methyl-2-butenal and p-aminophenol. The assay is effective in the submicromolar concentration range and can be used in crude plant extracts as well as in more highly purified preparations.

  7. Visualizing Capsaicinoids: Colorimetric Analysis of Chili Peppers (United States)

    Thompson, Robert Q.; Chu, Christopher; Gent, Robin; Gould, Alexandra P.; Rios, Laura; Vertigan, Theresa M.


    A colorimetric method for total capsaicinoids in chili pepper ("Capsicum") fruit is described. The placental material of the pepper, containing 90% of the capsaicinoids, was physically separated from the colored materials in the pericarp and extracted twice with methanol, capturing 85% of the remaining capsaicinoids. The extract, evaporated and…

  8. Novel colorimetric sensor for oral malodour

    Energy Technology Data Exchange (ETDEWEB)

    Alagirisamy, Nethaji; Hardas, Sarita S. [Hindustan Unilever Research Center, 64 Main Road, Whitefield, Bangalore 560066 (India); Jayaraman, Sujatha, E-mail: [Hindustan Unilever Research Center, 64 Main Road, Whitefield, Bangalore 560066 (India)


    Volatile sulphur compounds are the primary constituents of oral malodour. Quantitative tools for the detection of oral malodour are beneficial to evaluate the intensity of malodour, analyse its causes and monitor the effectiveness of customized treatments. We have developed an objective, cost effective, do-it-yourself colorimetric sensor for oral malodour quantification. The sensor consisted of a sensing solution, a gas sampling unit for collecting a known volume of mouth air and a photometric detector. The sensing solution was iodine and the depletion of iodine on reaction with hydrogen sulphide was detected colorimetrically using starch. The detection limit of the sensor is 0.05 {mu}g L{sup -1} of hydrogen sulphide, which is fit-for-purpose for oral malodour detection in healthy subjects as well as halitosis patients. Volatile sulphur compounds in mouth air were quantified in healthy human volunteers using this portable sensor and the detected levels were in the range of 0.2-0.4 {mu}g L{sup -1}. There was a good correlation between the VSC levels detected by the colorimetric sensor and halimeter (R{sup 2} = 0.934). The developed sensor can be easily fabricated in the laboratory, and it shows high potential to be used as a clinical evaluation tool for oral malodour assessments.

  9. Colorimetric evaluation of irradiated red beet roots

    Energy Technology Data Exchange (ETDEWEB)

    Nunes, Thaise C.F.; Rogovschi, Vladimir D.; Fabbri, Adriana D.T.; Sagretti, Juliana M.A.; Hirashima, Fabiana K.; Sabato, Susy F., E-mail: [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)


    The red beetroot contain antioxidant and anticancer activity and have been consumed all over the world. In order to increase the consumption of beetroot the food industry has created a practical alternative, a beetroot shaped like a small ball, minimally processed with the convenience in meal preparation. Food irradiation is in consonance with the proposal to increase the consumption of beetroot whilst maintaining quality and product safety. The aim of this study was to analyze changes in colorimetric properties in beetroot after the irradiation process. Samples of minimally processed beetroot were purchased at a local supermarket. The samples were exposed to gamma rays with doses of 1.0kG{sub y}, 2.0kG{sub y}, 3.0kG{sub y} and 4.0 kG{sub y} and were stored at 5 deg C. Colorimetric characteristics were analyzed such as L{sup *}, a{sup *}, b{sup *}, C{sup *}, h{sup *}, δE and WI. The results of the colorimetric evaluation showed no significant difference among the samples. The authors concluded that the treatment with low doses of gamma radiation keeps the quality of beetroot. (author)

  10. Colorimetric characterization models based on colorimetric characteristics evaluation for active matrix organic light emitting diode panels. (United States)

    Gong, Rui; Xu, Haisong; Tong, Qingfen


    The colorimetric characterization of active matrix organic light emitting diode (AMOLED) panels suffers from their poor channel independence. Based on the colorimetric characteristics evaluation of channel independence and chromaticity constancy, an accurate colorimetric characterization method, namely, the polynomial compensation model (PC model) considering channel interactions was proposed for AMOLED panels. In this model, polynomial expressions are employed to calculate the relationship between the prediction errors of XYZ tristimulus values and the digital inputs to compensate the XYZ prediction errors of the conventional piecewise linear interpolation assuming the variable chromaticity coordinates (PLVC) model. The experimental results indicated that the proposed PC model outperformed other typical characterization models for the two tested AMOLED smart-phone displays and for the professional liquid crystal display monitor as well.

  11. Colorimetric monitoring of formaldehyde in indoor environment using built-in camera on mobile phone. (United States)

    Sekine, Yoshika; Katori, Risa; Tsuda, Yuko; Kitahara, Takio


    A simple monitoring system of indoor air pollution is proposed by integrating a novel colorimetric detector of formaldehyde (HCHO) and a function of a built-in camera on mobile phone. The colorimetric detector employs a solid phase colorimetric reagent made from 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole, ZnO, KIO4 and agar, and changes colour from white to purple by exposure to HCHO gas. The degree of colour changes expressed in Red, Green and Blue model model responded to the HCHO concentration levels both in air and from building materials. Limit of quantitation of the detector with 24 h-exposure resulted in 0.011 mg/m(3) of air concentration which meets a requirement of methodology to detect indoor air quality guideline level of HCHO set by World Health Organization. The detector is also applicable to classify HCHO-emitting materials at least into Type 1, whose emission flux is greater than 120 μg/m(2)/h, and others. Then, variation of the acquired photo images was investigated by using various mobile phones and changing conditions of photography. As a result, the calibration of the measured colour intensity with a colour standard reduced the variation of the results and gave a significant output when the auto-focused images were taken under the condition of common indoor environment.

  12. Automatic sequences

    CERN Document Server

    Haeseler, Friedrich


    Automatic sequences are sequences which are produced by a finite automaton. Although they are not random they may look as being random. They are complicated, in the sense of not being not ultimately periodic, they may look rather complicated, in the sense that it may not be easy to name the rule by which the sequence is generated, however there exists a rule which generates the sequence. The concept automatic sequences has special applications in algebra, number theory, finite automata and formal languages, combinatorics on words. The text deals with different aspects of automatic sequences, in particular:· a general introduction to automatic sequences· the basic (combinatorial) properties of automatic sequences· the algebraic approach to automatic sequences· geometric objects related to automatic sequences.

  13. Suggested isosbestic wavelength calibration in clinical analyses. (United States)

    Hoxter, G


    I recommend the use of isosbestic points for conveniently checking the wavelength scale of spectrophotometers in the ultraviolet and visible regions. Colorimetric pH indicators, hemoglobin derivatives, and other radiation-absorbing substances that are convertible into stable isomers of different absorption spectra provide a means for calibrating many different wavelengths by comparing the absorptivities of these isomers in equimolar solutions. The method requires no special precautions and results are independent of substance concentration and temperature between 4 and 45 degrees C. Isosbestic calibration may be important for (e.g.) coenzyme-dependent dehydrogenase activity determinations and in quality assurance programs.


    Directory of Open Access Journals (Sweden)

    L. Barazzetti


    Full Text Available In photogrammetry a camera is considered calibrated if its interior orientation parameters are known. These encompass the principal distance, the principal point position and some Additional Parameters used to model possible systematic errors. The current state of the art for automated camera calibration relies on the use of coded targets to accurately determine the image correspondences. This paper presents a new methodology for the efficient and rigorous photogrammetric calibration of digital cameras which does not require any longer the use of targets. A set of images depicting a scene with a good texture are sufficient for the extraction of natural corresponding image points. These are automatically matched with feature-based approaches and robust estimation techniques. The successive photogrammetric bundle adjustment retrieves the unknown camera parameters and their theoretical accuracies. Examples, considerations and comparisons with real data and different case studies are illustrated to show the potentialities of the proposed methodology.

  15. Calibration uncertainty

    DEFF Research Database (Denmark)

    Heydorn, Kaj; Anglov, Thomas


    Methods recommended by the International Standardization Organisation and Eurachem are not satisfactory for the correct estimation of calibration uncertainty. A novel approach is introduced and tested on actual calibration data for the determination of Pb by ICP-AES. The improved calibration unce...

  16. Computer Vision Assisted Virtual Reality Calibration (United States)

    Kim, W.


    A computer vision assisted semi-automatic virtual reality (VR) calibration technology has been developed that can accurately match a virtual environment of graphically simulated three-dimensional (3-D) models to the video images of the real task environment.

  17. QXT-full Automatic Saccharify Instrument

    Institute of Scientific and Technical Information of China (English)


    QXT is a full automatic saccharify instrument of eight holes . The instrument use process control technology of micro-computer. It can realize automatic of saccharify full process correctly. Due to adapt control mode of high precision expert PID and digit automatic calibration technology of fill micro computer, not only ensure precision of linear raising temperature region (1 ℃ /min) and constant temperature region (temperature error ±0.2 ℃), but also overcome the disturbance

  18. Achromatic-chromatic colorimetric sensors for on-off type detection of analytes. (United States)

    Heo, Jun Hyuk; Cho, Hui Hun; Lee, Jin Woong; Lee, Jung Heon


    We report the development of achromatic colorimetric sensors; sensors changing their colors from achromatic black to other chromatic colors. An achromatic colorimetric sensor was prepared by mixing a general colorimetric indicator, whose color changes between chromatic colors, and a complementary colored dye with no reaction to the targeted analyte. As the color of an achromatic colorimetric sensor changes from black to a chromatic color, the color change could be much easily recognized than general colorimetric sensors with naked eyes. More importantly, the achromatic colorimetric sensors enable on-off type recognition of the presence of analytes, which have not been achieved from most colorimetric sensors. In addition, the color changes from some achromatic colorimetric sensors (achromatic Eriochrome Black T and achromatic Benedict's solution) could be recognized with naked eyes at much lower concentration ranges than normal chromatic colorimetric sensors. These results provide new opportunities in the use of colorimetric sensors for diverse applications, such as harsh industrial, environmental, and biological detection.

  19. Colorimetric characterization of imaging device by total color difference minimization

    Institute of Scientific and Technical Information of China (English)

    MOU Tong-sheng; SHEN Hui-liang


    Colorimetric characterization is to transform the device-dependent responses to device-independent colorimetric values, and is usually conducted in CIEXYZ space. However, the optimal solution in CIEXYZ space does not mean the minimization of perceptual error. A novel method for colorimetric characterization of imaging device based on the minimization of total color difference is proposed. The method builds the transform between RGB space and CIELAB space directly using the downhill simplex algorithm. Experimental results showed that the proposed method performs better than traditional least-square (LS) and total-least-square (TLS) methods, especially for colors with low luminance values.

  20. Accurate colorimetric feedback for RGB LED clusters (United States)

    Man, Kwong; Ashdown, Ian


    We present an empirical model of LED emission spectra that is applicable to both InGaN and AlInGaP high-flux LEDs, and which accurately predicts their relative spectral power distributions over a wide range of LED junction temperatures. We further demonstrate with laboratory measurements that changes in LED spectral power distribution with temperature can be accurately predicted with first- or second-order equations. This provides the basis for a real-time colorimetric feedback system for RGB LED clusters that can maintain the chromaticity of white light at constant intensity to within +/-0.003 Δuv over a range of 45 degrees Celsius, and to within 0.01 Δuv when dimmed over an intensity range of 10:1.

  1. Design and Implementation of A Circuit Board Calibration System

    Directory of Open Access Journals (Sweden)

    Bai Hang


    Full Text Available With the development of science and technology, the traditional artificial detection methods cannot meet the requirements of modern equipment testing and calibration. Combined with the actual demand, a kind of circuit boards calibration system are put forward. It can to realize automatic testing and calibration of the circuit boards. Many functions of the calibration system such as automatic testing, self-test and monitoring are summarized. The hardware is introduced which including the industrial computer system, calibration adapter and so on. Then, development platform, the thought of program design and the structure of the software are introduced in detail. The function of automatic calibration to specific circuit boards are realized. Because the system has good commonality and easy to extend to upgrade, the development ideas and experiences can be applied to similar circuit boards automatic testing system.

  2. 3D laser methods for calibrating and localising robotic vehicles


    Mark Sheehan


    This thesis is about the construction and automatic target-less calibration of a 3D laser sensor; this is then used to localise an autonomous vehicle without using other sensors. Two novel contributions to our knowledge of robotics are presented here. The first is an automatic calibration routine, which is capable of learning its calibration parameters using only data from a 3D laser scanner. Targets with known dimensions are not required, as has previously been the case. The second main ...

  3. A highly sensitive colorimetric and ratiometric sensor for fluoride ion

    Institute of Scientific and Technical Information of China (English)

    Zhao Wu Xu; Jin Tang; He Tian


    A new benzoimidazole-naphthalimide derivative 4 was synthesized and its photophysical properties were studied.This compound showed highly selectively and sensitive colorimetric and ratiometric sensing ability for fluoride anion.

  4. Simple Colorimetric Sensor for Trinitrotoluene Testing (United States)

    Samanman, S.; Masoh, N.; Salah, Y.; Srisawat, S.; Wattanayon, R.; Wangsirikul, P.; Phumivanichakit, K.


    A simple operating colorimetric sensor for trinitrotoluene (TNT) determination using a commercial scanner as a captured image was designed. The sensor is based on the chemical reaction between TNT and sodium hydroxide reagent to produce the color change within 96 well plates, which observed finally, recorded using a commercial scanner. The intensity of the color change increased with increase in TNT concentration and could easily quantify the concentration of TNT by digital image analysis using the Image J free software. Under optimum conditions, the sensor provided a linear dynamic range between 0.20 and 1.00 mg mL-1(r = 0.9921) with a limit of detection of 0.10± 0.01 mg mL-1. The relative standard deviation for eight experiments for the sensitivity was 3.8%. When applied for the analysis of TNT in two soil extract samples, the concentrations were found to be non-detectable to 0.26±0.04 mg mL-1. The obtained recovery values (93-95%) were acceptable for soil samples tested.

  5. A colorimetric sensor array of porous pigments. (United States)

    Lim, Sung H; Kemling, Jonathan W; Feng, Liang; Suslick, Kenneth S


    The development of a low-cost, simple colorimetric sensor array capable of the detection and identification of toxic gases is reported. This technology uses a disposable printed array of porous pigments in which metalloporphyrins and chemically-responsive dyes are immobilized in a porous matrix of organically modified siloxanes (ormosils) and printed on a porous membrane. The printing of the ormosil into the membrane is highly uniform and does not lessen the porosity of the membrane, as shown by scanning electron microscopy. When exposed to an analyte, these pigments undergo reactions that result in well-defined color changes due to strong chemical interactions: ligation to metal ions, Lewis or Brønsted acid-base interactions, hydrogen bonding, etc. Striking visual identification of 3 toxic gases has been shown at the IDLH (immediately dangerous to life and health) concentration, at the PEL (permissible exposure level), and at a level well below the PEL. Identification and quantification of analytes were achieved using the color change profiles, which were readily distinguishable in a hierarchical clustering analysis (HCA) dendrogram, with no misclassifications in 50 trials.

  6. Photoacoustic and Colorimetric Visualization of Latent Fingerprints. (United States)

    Song, Kai; Huang, Peng; Yi, Chenglin; Ning, Bo; Hu, Song; Nie, Liming; Chen, Xiaoyuan; Nie, Zhihong


    There is a high demand on a simple, rapid, accurate, user-friendly, cost-effective, and nondestructive universal method for latent fingerprint (LFP) detection. Herein, we describe a combination imaging strategy for LFP visualization with high resolution using poly(styrene-alt-maleic anhydride)-b-polystyrene (PSMA-b-PS) functionalized gold nanoparticles (GNPs). This general approach integrates the merits of both colorimetric imaging and photoacoustic imaging. In comparison with the previous methods, our strategy is single-step and does not require the signal amplification by silver staining. The PSMA-b-PS functionalized GNPs have good stability, tunable color, and high affinity for universal secretions (proteins/polypeptides/amino acids), which makes our approach general and flexible for visualizing LFPs on different substrates (presumably with different colors) and from different people. Moreover, the unique optical property of GNPs enables the photoacoustic imaging of GNPs-deposited LFPs with high resolution. This allows observation of level 3 hyperfine features of LFPs such as the pores and ridge contours by photoacoustic imaging. This technique can potentially be used to identify chemicals within LFP residues. We believe that this dual-modality imaging of LFPs will find widespread use in forensic investigations and medical diagnostics.

  7. Colorimetric Sensor Array for White Wine Tasting. (United States)

    Chung, Soo; Park, Tu San; Park, Soo Hyun; Kim, Joon Yong; Park, Seongmin; Son, Daesik; Bae, Young Min; Cho, Seong In


    A colorimetric sensor array was developed to characterize and quantify the taste of white wines. A charge-coupled device (CCD) camera captured images of the sensor array from 23 different white wine samples, and the change in the R, G, B color components from the control were analyzed by principal component analysis. Additionally, high performance liquid chromatography (HPLC) was used to analyze the chemical components of each wine sample responsible for its taste. A two-dimensional score plot was created with 23 data points. It revealed clusters created from the same type of grape, and trends of sweetness, sourness, and astringency were mapped. An artificial neural network model was developed to predict the degree of sweetness, sourness, and astringency of the white wines. The coefficients of determination (R2) for the HPLC results and the sweetness, sourness, and astringency were 0.96, 0.95, and 0.83, respectively. This research could provide a simple and low-cost but sensitive taste prediction system, and, by helping consumer selection, will be able to have a positive effect on the wine industry.

  8. Colorimetric Sensor Array for White Wine Tasting

    Directory of Open Access Journals (Sweden)

    Soo Chung


    Full Text Available A colorimetric sensor array was developed to characterize and quantify the taste of white wines. A charge-coupled device (CCD camera captured images of the sensor array from 23 different white wine samples, and the change in the R, G, B color components from the control were analyzed by principal component analysis. Additionally, high performance liquid chromatography (HPLC was used to analyze the chemical components of each wine sample responsible for its taste. A two-dimensional score plot was created with 23 data points. It revealed clusters created from the same type of grape, and trends of sweetness, sourness, and astringency were mapped. An artificial neural network model was developed to predict the degree of sweetness, sourness, and astringency of the white wines. The coefficients of determination (R2 for the HPLC results and the sweetness, sourness, and astringency were 0.96, 0.95, and 0.83, respectively. This research could provide a simple and low-cost but sensitive taste prediction system, and, by helping consumer selection, will be able to have a positive effect on the wine industry.

  9. Basic design principles of colorimetric vision systems (United States)

    Mumzhiu, Alex M.


    Color measurement is an important part of overall production quality control in textile, coating, plastics, food, paper and other industries. The color measurement instruments such as colorimeters and spectrophotometers, used for production quality control have many limitations. In many applications they cannot be used for a variety of reasons and have to be replaced with human operators. Machine vision has great potential for color measurement. The components for color machine vision systems, such as broadcast quality 3-CCD cameras, fast and inexpensive PCI frame grabbers, and sophisticated image processing software packages are available. However the machine vision industry has only started to approach the color domain. The few color machine vision systems on the market, produced by the largest machine vision manufacturers have very limited capabilities. A lack of understanding that a vision based color measurement system could fail if it ignores the basic principles of colorimetry is the main reason for the slow progress of color vision systems. the purpose of this paper is to clarify how color measurement principles have to be applied to vision systems and how the electro-optical design features of colorimeters have to be modified in order to implement them for vision systems. The subject of this presentation far exceeds the limitations of a journal paper so only the most important aspects will be discussed. An overview of the major areas of applications for colorimetric vision system will be discussed. Finally, the reasons why some customers are happy with their vision systems and some are not will be analyzed.

  10. Direct Quantification of Carotenoids in Low Fat Baby Foods Via Laser Photoacoustics and Colorimetric Index * (United States)

    Dóka, O.; Ajtony, Zs.; Bicanic, D.; Valinger, D.; Végvári, Gy.


    Carotenoids are important antioxidants found in various foods including those for nutrition of infants. In this investigation, the total carotenoid content (TCC) of nine different commercially available baby foods was quantified using colorimetric index * obtained via reflectance colorimetry (RC) and by laser photoacoustic spectroscopy (LPAS) at 473 nm. The latter requires a minimum of sample preparation and only a one time calibration step which enables practically direct quantification of TCC. Results were verified versus UV-Vis spectrophotometry (SP) as the reference technique. It was shown that RC and LPAS (at 473 nm) provide satisfactory results for *, = 0.9925 and = 0.9972, respectively. Other color indices do not show a correlation with TCC. When determining the TCC in baby foods containing tomatoes, it is necessary to select a different analytical wavelength to compensate for the effect of lycopene's presence in the test samples.

  11. Automatic Reading

    Institute of Scientific and Technical Information of China (English)



    <正>Reading is the key to school success and,like any skill,it takes practice.A child learns to walk by practising until he no longer has to think about how to put one foot in front of the other.The great athlete practises until he can play quickly,accurately and without thinking.Ed- ucators call it automaticity.

  12. Input calibration for negative originals (United States)

    Tuijn, Chris


    One of the major challenges in the prepress environment consists of controlling the electronic color reproduction process such that a perfect match of any original can be realized. Whether this goal can be reached depends on many factors such as the dynamic range of the input device (scanner, camera), the color gamut of the output device (dye sublimation printer, ink-jet printer, offset), the color management software etc. The characterization of the color behavior of the peripheral devices is therefore very important. Photographs and positive transparents reflect the original scene pretty well; for negative originals, however, there is no obvious link to either the original scene or a particular print of the negative under consideration. In this paper, we establish a method to scan negatives and to convert the scanned data to a calibrated RGB space, which is known colorimetrically. This method is based on the reconstruction of the original exposure conditions (i.e., original scene) which generated the negative. Since the characteristics of negative film are quite diverse, a special calibration is required for each combination of scanner and film type.

  13. Emergency First Responders' Experience with Colorimetric Detection Methods

    Energy Technology Data Exchange (ETDEWEB)

    Sandra L. Fox; Keith A. Daum; Carla J. Miller; Marnie M. Cortez


    Nationwide, first responders from state and federal support teams respond to hazardous materials incidents, industrial chemical spills, and potential weapons of mass destruction (WMD) attacks. Although first responders have sophisticated chemical, biological, radiological, and explosive detectors available for assessment of the incident scene, simple colorimetric detectors have a role in response actions. The large number of colorimetric chemical detection methods available on the market can make the selection of the proper methods difficult. Although each detector has unique aspects to provide qualitative or quantitative data about the unknown chemicals present, not all detectors provide consistent, accurate, and reliable results. Included here, in a consumer-report-style format, we provide “boots on the ground” information directly from first responders about how well colorimetric chemical detection methods meet their needs in the field and how they procure these methods.

  14. Development and calibration automatic equipment's measuring in real time of the environmental radioactivity by gamma spectrometry; Desarrollo y calibracion de equipos automaticos de medida en tiempo real de la radiactividad ambiental mediante espectrometria gamma

    Energy Technology Data Exchange (ETDEWEB)

    Casanovas, R.; Morant, J. J.; Salvado, M.


    In this paper, we describe the general aspects of the implementation of gamma spectrometry in water, as well as the development of two measuring devices based on this technique: aerosol monitor (RARM-F) and a monitor direct measurement (RARMD2) , both patent applications. Furthermore, they described in detail the aspects of calibration of equipment, which has been made by combining experimental measurements with Monte Carlo simulations. (Author)

  15. A colorimetric tetrathiafulvalene-calix 4 pyrrole anion sensor

    DEFF Research Database (Denmark)

    Nielsen, K. A.


    The interaction and colorimetric sensing properties of a tetrathiafulvalene substituted calix[4]pyrrole sensor with anions were investigated using H-1 NMR and absorption spectroscopic techniques. Visual color changes were observed upon addition of different anions (Cl-, Br-, CN-, and AcO-) to a s......The interaction and colorimetric sensing properties of a tetrathiafulvalene substituted calix[4]pyrrole sensor with anions were investigated using H-1 NMR and absorption spectroscopic techniques. Visual color changes were observed upon addition of different anions (Cl-, Br-, CN-, and Ac......O-) to a solution of the sensor. (C) 2012 Elsevier Ltd. All rights reserved....

  16. A quick colorimetric method for total lipid quantification in microalgae. (United States)

    Byreddy, Avinesh R; Gupta, Adarsha; Barrow, Colin J; Puri, Munish


    Discovering microalgae with high lipid productivity are among the key milestones for achieving sustainable biodiesel production. Current methods of lipid quantification are time intensive and costly. A rapid colorimetric method based on sulfo-phospho-vanillin (SPV) reaction was developed for the quantification of microbial lipids to facilitate screening for lipid producing microalgae. This method was successfully tested on marine thraustochytrid strains and vegetable oils. The colorimetric method results correlated well with gravimetric method estimates. The new method was less time consuming than gravimetric analysis and is quantitative for lipid determination, even in the presence of carbohydrates, proteins and glycerol.

  17. Colorimetric detection of Cucumber green mottle mosaic virus using unmodified gold nanoparticles as colorimetric probes. (United States)

    Wang, Lin; Liu, Zhanmin; Xia, Xueying; Yang, Cuiyun; Huang, Junyi; Wan, Sibao


    Cucumber green mottle mosaic virus (CGMMV)causes a severe mosaic symptom of watermelon and cucumber, and can be transmitted via infected cucumber seeds, leaves and soil. It remains a challenge to detect this virus to prevent its introduction and infection and spread in fields. For this purpose, a simple and sensitive label-free colorimetric detection method for CGMMV has been developed with unmodified gold nanoparticles (AuNPs) as colorimetric probes. The method is based on the finding that the presence of RT-PCR target products of CGMMV and species-specific probes results in color change of AuNPs from red to blue after NaCl induction. Normally, species-specific probes attach to the surface of AuNPs and thereby increasing their resistance to NaCl-induced aggregation. The concentration of sodium, probes in the reaction system and evaluation of specificity and sensitivity of a novel assay, visual detection of Cucumber green mottle mosaic virus using unmodified AuNPs has been carried out with simple preparation of samples in our study. Through this assay, as low as 30pg/μL of CGMMV RNA was thus detected visually, by the naked eye, without the need for any sophisticated, expensive instrumentation and biochemical reagents. The specificity was 100% and exhibited good reproducibility in our assays. The results note that this assay is highly species-specific, simple, low-cost, and visual for easy detection of CGMMV in plant tissues. Therefore, visual assay is a potentially useful tool for middle or small-scales corporations and entry-exit inspection and quarantine bureau to detect CGMMV in cucumber seeds or plant tissues.

  18. Selectively colorimetric detection of cysteine with triangular silver nanoprisms

    Institute of Scientific and Technical Information of China (English)

    Tong Wu; Yuan Fang Li; Cheng Zhi Huang


    Triangular silver nanoprisms were prepared and applied to make colorimetric detection of cysteine based on our findings that cysteine could lead to the blue shift of the dipole plasmon resonance absorption,but other 19 kinds of natural amino acids could not.Cysteine with a concentration 160 nmol/L can result in a color change that can be discerned with naked eyes.

  19. Capillarity-based preparation system for optical colorimetric sensor arrays. (United States)

    Luo, Xiao-Gang; Yi, Xin; Bu, Xiang-Nan; Hou, Chang-Jun; Huo, Dan-Qun; Yang, Mei; Fa, Huan-Bao; Lei, Jin-Can


    In recent years, optical colorimetric sensor arrays have demonstrated beneficial features, including rapid response, high selectivity, and high specificity; as a result, it has been extensively applied in food inspection and chemical studies, among other fields. There are instruments in the current market available for the preparation of an optical colorimetric sensor array, but it lacks the corresponding research of the preparation mechanism. Therefore, in connection with the main features of this kind of sensor array such as consistency, based on the preparation method of contact spotting, combined with a capillary fluid model, Washburn equation, Laplace equation, etc., this paper develops a diffusion model of an optical colorimetric sensor array during its preparation and sets up an optical colorimetric sensor array preparation system based on this diffusion model. Finally, this paper compares and evaluates the sensor arrays prepared by the system and prepared manually in three aspects such as the quality of array point, response of array, and response result, and the results show that the performance index of the sensor array prepared by a system under this diffusion model is better than that of the sensor array of manual spotting, which meets the needs of the experiment.

  20. Photonic Crystal Structures with Tunable Structure Color as Colorimetric Sensors

    Directory of Open Access Journals (Sweden)

    Ke-Qin Zhang


    Full Text Available Colorimetric sensing, which transduces environmental changes into visible color changes, provides a simple yet powerful detection mechanism that is well-suited to the development of low-cost and low-power sensors. A new approach in colorimetric sensing exploits the structural color of photonic crystals (PCs to create environmentally-influenced color-changeable materials. PCs are composed of periodic dielectrics or metallo-dielectric nanostructures that affect the propagation of electromagnetic waves (EM by defining the allowed and forbidden photonic bands. Simultaneously, an amazing variety of naturally occurring biological systems exhibit iridescent color due to the presence of PC structures throughout multi-dimensional space. In particular, some kinds of the structural colors in living organisms can be reversibly changed in reaction to external stimuli. Based on the lessons learned from natural photonic structures, some specific examples of PCs-based colorimetric sensors are presented in detail to demonstrate their unprecedented potential in practical applications, such as the detections of temperature, pH, ionic species, solvents, vapor, humidity, pressure and biomolecules. The combination of the nanofabrication technique, useful design methodologies inspired by biological systems and colorimetric sensing will lead to substantial developments in low-cost, miniaturized and widely deployable optical sensors.

  1. Capillarity-based preparation system for optical colorimetric sensor arrays (United States)

    Luo, Xiao-gang; Yi, Xin; Bu, Xiang-nan; Hou, Chang-jun; Huo, Dan-qun; Yang, Mei; Fa, Huan-bao; Lei, Jin-can


    In recent years, optical colorimetric sensor arrays have demonstrated beneficial features, including rapid response, high selectivity, and high specificity; as a result, it has been extensively applied in food inspection and chemical studies, among other fields. There are instruments in the current market available for the preparation of an optical colorimetric sensor array, but it lacks the corresponding research of the preparation mechanism. Therefore, in connection with the main features of this kind of sensor array such as consistency, based on the preparation method of contact spotting, combined with a capillary fluid model, Washburn equation, Laplace equation, etc., this paper develops a diffusion model of an optical colorimetric sensor array during its preparation and sets up an optical colorimetric sensor array preparation system based on this diffusion model. Finally, this paper compares and evaluates the sensor arrays prepared by the system and prepared manually in three aspects such as the quality of array point, response of array, and response result, and the results show that the performance index of the sensor array prepared by a system under this diffusion model is better than that of the sensor array of manual spotting, which meets the needs of the experiment.

  2. Haussdorff and hellinger for colorimetric sensor array classification

    DEFF Research Database (Denmark)

    Alstrøm, Tommy Sonne; Jensen, Bjørn Sand; Schmidt, Mikkel Nørgaard;


    Development of sensors and systems for detection of chemical compounds is an important challenge with applications in areas such as anti-terrorism, demining, and environmental monitoring. A newly developed colorimetric sensor array is able to detect explosives and volatile organic compounds...

  3. Evaluation of a Colorimetric Personal Dosimeter for Nitrogen Oxide. (United States)

    Diamond, Philip

    A personal colorimetric dosimeter for nitrogen dioxide was developed. Tests were performed to determine the response of these strips to various concentrations of NO2. The dosimeter strips were satisfactory for approximate determinations of total exposure (concentration + time) of nitrogen dioxide. The total exposure was calculated in terms of time…

  4. Testing of Colorimetric Tubes for Nitrogen Dioxide and Monomethylhydrazine. (United States)

    Diamond, Philip

    Colorimetric tubes for nitrogen dioxide (NO2) and monomethylhydrazine (MMH) were tested for accuracy and results indicate that at the levels checked the tubes' average deviation was plus or minus 20 percent. Tube NO2 concentrations all read lower than the analyzed concentrations. MMH tubes read much higher than the analyzed concentration of 0.28…

  5. A modified colorimetric method for phytic acid analysis in soybean (United States)

    A quantitative, reproducible, and efficient phytic acid assay procedure is needed to screen breeding populations and support genetic studies in soybeans. The objective of this study was to modify the colorimetric Wade reagent method and compare the accuracy and applicability of this new method in de...

  6. Multi-colorimetric sensor array for detection of illegal materials

    DEFF Research Database (Denmark)

    Kostesha, Natalie; Boisen, Anja; Jakobsen, Mogens Havsteen;


    and signal processing enhancements to sensing methods, recognition ability, data acquisition time and data processing algorithms are necessary. In this research we work towards the development of a rapid, easy in use, highly sensitive, specific (minimal false positives) sensor based on a colorimetric sensing...

  7. Color Responses of a Tripodal Colorimetric Sensor toward Anions

    Institute of Scientific and Technical Information of China (English)

    WEI Lan-Hua; HE Yong-Bing; WU Jin-Long; QIN Hai-Juan; XU Kuo-Xi; MENG Ling-Zhi


    A thiourea-based tripodal colorimetric anion sensor was synthesized. Its binding abilities with AcO- and halide anions in DMSO were studied by UV-Vis spectra. The sensor showed different color responses to these anions. The association constants and different stoichiometries were deduced by nonlinear least-square curve fitting or linear fitting.

  8. Colorimetric and fluorescent detection of biological thiols in aqueous solution

    Institute of Scientific and Technical Information of China (English)

    Yin-Hui Li; Jin-Feng Yang; Chang-Hui Liu; Ji-Shan Li; Rong-Hua Yang


    A new colorimetric and fluorescent probe,2-(2,4-dinitrostyryl)-1,3,3-trimethyl-3H-indolium iodide (DTI),for selective and sensitive detection of biological thiols is reported.In aqueous solution at physiological pH 7.4,biological thiols react with DTI via Michael addition to give the brownish red adduct concomitant with fluorescence emission decrease.

  9. High-resolution colorimetric imaging of paintings (United States)

    Martinez, Kirk; Cupitt, John; Saunders, David R.


    With the aim of providing a digital electronic replacement for conventional photography of paintings, a scanner has been constructed based on a 3000 X 2300 pel resolution camera which is moved precisely over a 1 meter square area. Successive patches are assembled to form a mosaic which covers the whole area at c. 20 pels/mm resolution, which is sufficient to resolve the surface textures, particularly craquelure. To provide high color accuracy, a set of seven broad-band interference filters are used to cover the visible spectrum. A calibration procedure based upon a least-mean-squares fit to the color of patches from a Macbeth Colorchecker chart yields an average color accuracy of better than 3 units in the CMC uniform color space. This work was mainly carried out as part of the VASARI project funded by the European Commission's ESPRIT program, involving companies and galleries from around Europe. The system is being used to record images for conservation research, for archival purposes and to assist in computer-aided learning in the field of art history. The paper will describe the overall system design, including the selection of the various hardware components and the design of controlling software. The theoretical basis for the color calibration methodology is described as well as the software for its practical implementation. The mosaic assembly procedure and some of the associated image processing routines developed are described. Preliminary results from the research will be presented.

  10. The KLOE Online Calibration System

    Institute of Scientific and Technical Information of China (English)



    Based on all the features of the KLOE online software,the online calibration system performs current calibration quality checking in real time and starts automatically new calibration procedures when needed.Acalibration manager process controls the system,implementing the interface to the online system,receiving information from the run control and translating its state transitions to a separate state machine.It acts as a " calibration run controller"and performs failure recovery when requested by a set of process checkers.The core of the system is a multi-threaded OO histogram server that receives histogramming commands by remote processes and operates on local ROOT histograms.A client library and C,fortran and C++ application interface libraries allow the user to connect and define his own histogram or read histograms owned by others using an bool-like interface.Several calibration processes running in parallel in a destributed,multiplatform environment can fill the same histograms,allowing fast external information check.A monitor thread allow remote browsing for visual inspection,Pre-filtered data are read in nonprivileged spy mode from the data acquisition system via the Kloe Integrated Dataflow,privileged spy mode from the data acquisiton system via the Kole Integrated Dataflow.The main characteristics of the system are presented.

  11. Automated intraoperative calibration for prostate cancer brachytherapy

    Energy Technology Data Exchange (ETDEWEB)

    Kuiran Chen, Thomas; Heffter, Tamas; Lasso, Andras; Pinter, Csaba; Abolmaesumi, Purang; Burdette, E. Clif; Fichtinger, Gabor [Queen' s University, Kingston, Ontario K7L 3N6 (Canada); University of British Columbia, Vancouver, British Columbia V6T 1Z4 (Canada); Acoustic MedSystems, Inc., Champaign, Illinois 61820-3979 (United States); Queen' s University, Kingston, Ontario K7L 3N6 (Canada) and Johns Hopkins University, Baltimore, Maryland 21218-2682 (United States)


    Purpose: Prostate cancer brachytherapy relies on an accurate spatial registration between the implant needles and the TRUS image, called ''calibration''. The authors propose a new device and a fast, automatic method to calibrate the brachytherapy system in the operating room, with instant error feedback. Methods: A device was CAD-designed and precision-engineered, which mechanically couples a calibration phantom with an exact replica of the standard brachytherapy template. From real-time TRUS images acquired from the calibration device and processed by the calibration system, the coordinate transformation between the brachytherapy template and the TRUS images was computed automatically. The system instantly generated a report of the target reconstruction accuracy based on the current calibration outcome. Results: Four types of validation tests were conducted. First, 50 independent, real-time calibration trials yielded an average of 0.57 {+-} 0.13 mm line reconstruction error (LRE) relative to ground truth. Second, the averaged LRE was 0.37 {+-} 0.25 mm relative to ground truth in tests with six different commercial TRUS scanners operating at similar imaging settings. Furthermore, testing with five different commercial stepper systems yielded an average of 0.29 {+-} 0.16 mm LRE relative to ground truth. Finally, the system achieved an average of 0.56 {+-} 0.27 mm target registration error (TRE) relative to ground truth in needle insertion tests through the template in a water tank. Conclusions: The proposed automatic, intraoperative calibration system for prostate cancer brachytherapy has achieved high accuracy, precision, and robustness.

  12. Surface plasmon resonance based selective and sensitive colorimetric determination of azithromycin using unmodified silver nanoparticles in pharmaceuticals and human plasma (United States)

    Chavada, Vijay D.; Bhatt, Nejal M.; Sanyal, Mallika; Shrivastav, Pranav S.


    In this article we report a novel method for colorimetric sensing and selective determination of a non-chromophoric drug-azithromycin, which lacks native absorbance in the UV-Visible region using unmodified silver nanoparticles (AgNPs). The citrate-capped AgNps dispersed in water afforded a bright yellow colour owing to the electrostatic repulsion between the particles due to the presence of negatively charged surface and showed surface plasmon resonance (SPR) band at 394 nm. Addition of positively charged azithromycin at a concentration as low as 0.2 μM induced rapid aggregation of AgNPs by neutralizing the negative charge on the particle surface. This phenomenon resulted in the colour change from bright yellow to purple which could be easily observed by the naked eye. This provided a simple platform for rapid determination of azithromycin based on colorimetric measurements. The factors affecting the colorimetric response like pH, volume of AgNPs suspension and incubation time were suitably optimized. The validated method was found to work efficiently in the established concentration range of 0.2-100.0 μM using two different calibration models. The selectivity of the method was also evaluated by analysis of nanoparticles-aggregation response upon addition of several anions, cations and some commonly prescribed antibiotics. The method was successfully applied for the analysis of azithromycin in pharmaceuticals and spiked human plasma samples with good accuracy and precision. The simplicity, efficiency and cost-effectiveness of the method hold tremendous potential for the analysis of such non-chromophoric pharmaceuticals.

  13. Calibration and study of the measurement capabilities of real-time gamma spectrometry equipment developed for the renewal of the network of stations automatic monitoring environmental radiation of the Generalitat of Catalunya; Calibracion y estudio de las capacidades de medida de los equipos de espectrometria gamma en tiempo real desarrollados para la renovacion de la Red de Estaciones Automaticas de vigilancia radiologia ambiental de la Generalitat de Catalunya

    Energy Technology Data Exchange (ETDEWEB)

    Casanovas, R.; Prieto, E.; Salvado, M.


    The renewal of the automatic environmental radiation surveillance network of the Generalitat of Catalunya has been carried out through the development and implementation of gamma-spectrometry-based equipment. the monitors use scintillation crystals, either Na(TI) or LaBr{sub 3}(Ce) and currently, there are 3 types of equipment water radioactivity monitors, aerosols on a particulate filter monitors and direct measurement monitors. In this paper, we expose the basic features of its operation, the details of their calibration and the minimum detectable activity concentration for some isotopes. (Author)

  14. Calibration and study of the measurement capabilities of real-time gamma spectrometry equipment, developed for the renewal of the network of automatic stations for environmental radiological surveillance of the Generalitat de Catalunya; Calibracion y estudio de las capacidades de medida de loes equipos de espectrometria gamma en tiempo real desarrollados para la renovacion de la Red de Estaciones Automaticas de vigencia radiologica ambiental de la generalitat de Catalunya

    Energy Technology Data Exchange (ETDEWEB)

    Casanovas, R.; Prieto, E.; Salvado, M.


    The renewal of the automatic environmental radiation surveillance network of the Gereralitat of Catalunya has been carried out through the development and implementation of gamma-spectrometry-based equipment. The monitors use scintillation cristals, either NaI(Tl) or LaBr{sub 3}(Ce) and currently, there are 3 types of equipment: water radioactivity monitors, aerosols on a particulate filter monitors and direct measurement monitors. In this paper, we expose the basic features of its operation, the details of their calibration and the minimum detectable activity concentrations for some isotopes.

  15. Colorimetric determination of melamine in milk using unmodified silver nanoparticles (United States)

    Kumar, Naveen; Kumar, Harish; Mann, Bimlesh; Seth, Raman


    Melamine is nitrogen rich chemical compound used as an adulterant in dairy products by unscrupulous people to increase the apparent protein content. This incident prompted the researchers to develop simple methods for easy detection of melamine in food samples. In the present paper, we report a simple and sensitive colorimetric method for detection of melamine in milk based on silver nanoparticles. This method relies upon the principle that melamine causes the aggregation of silver nanoparticles, resulting in abrupt color change from yellow to red under optimized conditions. The concentration of melamine in adulterated sample can be quantitated by monitoring the absorption spectra of silver nanoparticles using ultraviolet-visible (UV-Vis) spectrometer. The present colorimetric method which utilizes silver nanoparticles of 35 nm can reliably detect melamine down to a concentration of 0.04 mg l- 1.

  16. 65 nm CMOS Sensors Applied to Mathematically Exact Colorimetric Reconstruction

    CERN Document Server

    Mayr, C; Krause, A; Schlüßler, J -U; Schüffny, R


    Extracting colorimetric image information from the spectral characteristics of image sensors is a key issue in accurate image acquisition. Technically feasible filter/sensor combinations usually do not replicate colorimetric responses with sufficient accuracy to be directly applicable to color representation. A variety of transformations have been proposed in the literature to compensate for this. However, most of those rely on heuristics and/or introduce a reconstruction dependent on the composition of the incoming illumination. In this work, we present a spectral reconstruction method that is independent of illumination and is derived in a mathematically strict way. It provides a deterministic method to arrive at a least mean squared error approximation of a target spectral characteristic from arbitrary sensor response curves. Further, we present a new CMOS sensor design in a standard digital 65nm CMOS technology. Novel circuit techniques are used to achieve performance comparable with much larger-sized spe...

  17. Colorimetric measurements as control elements in wood conservation status

    Directory of Open Access Journals (Sweden)

    Ovidia Soto-Martín


    Full Text Available This paper is a methodological proposal for the study of altarpieces on wooden supports. The process was implemented to study the altarpiece of San Antonio de Padua in Garachico, Tenerife. For this, we conducted a review of key aspects appropriate to the discipline of wood identification carried out by macroscopic examination and for the characterization of the status of deterioration by colorimetric analysis. For the evaluation of the wood conservation status, the samples were subjected for the first time to colorimetric measurement. As a result we have created an online database to provide information for conservation professionals permitting them to design a proposal for preventive conservation and intervention individually for each object.

  18. Autodepuração de cursos d´água: um programa de modelagem Streeter Phelps com calibração automática e correção de anaerobiose Self-depuration in watercourses: a program for Streeter Phelps modeling with automatic calibration and correction for anaerobic conditions

    Directory of Open Access Journals (Sweden)

    Iury Steiner de Oliveira Bezerra


    Full Text Available Foi desenvolvido um programa usando Object Pascal e Matlab, para o modelo de Streeter Phelps de autodepuração. Foram incorporadas condições de contorno, sugeridas por Chapra, que propiciam: a simulação do perfil de OD e DBO5 considerando várias entradas de efluentes ao longo do curso d'água e a correção para condições de anaerobiose, condições estas que contribuem com o surgimento de concentrações negativas de OD e conduzem a previsões errôneas. Foi, também, incorporada uma metodologia de calibração automática, baseada na minimização dos erros quadráticos entre as concentrações calculadas e observadas, para obtenção e escolha dos valores dos coeficientes de desoxigenação e reaeração. Para o teste do programa, foram feitas várias simulações: uma para testar o método de calibração automática e outras duas para testar as condições de contorno. Em todos os casos, os resultados obtidos produziram bons desempenhos. O aplicativo executável servirá como ferramenta auxiliar no meio acadêmico, como recurso didático, em aulas de saneamento ambiental, e como ferramenta de gestão da qualidade das águas.Using Object Pascal and Matlab, a program was developed for Streeter Phelps modeling of self-purification. Boundary conditions suggested by Chapra were incorporated that permit the simulation of DO and BOD5 profiles considering multiple point sources along the flow path and a correction for conditions of anaerobiosis, which lead to negative DO and erroneous previsions in Streeter Phelps modeling. A method of automatic calibration, based on minimizing square deviations between simulated and observed concentrations, for obtaining the coefficients of deoxygenation and reaeration, was incorporated also. Various simulations for testing the program were performed: one for automatic calibration and two for boundary conditions. In all cases a good performance was obtained. The program may serve equally as a teaching

  19. Colorimetric detection of mercury species based on functionalized gold nanoparticles. (United States)

    Chen, Ling; Li, Jinhua; Chen, Lingxin


    The speciation analysis of heavy metal pollutants is very important because different species induce different toxicological effects. Nanomaterial-assisted optical sensors have achieved rapid developments, displaying wide applications to heavy metal ions but few to metal speciation analysis. In this work, a novel colorimetric nanosensor strategy for mercury speciation was proposed for the first time, based on the analyte-induced aggregation of gold nanoparticles (Au NPs) with the assistance of a thiol-containing ligand of diethyldithiocarbamate (DDTC). Upon the addition of mercury species, because Hg-DDTC was more stable than Cu-DDTC, a place-displacement between Hg species and Cu(2+) would occur, and thereby the functionalized Au NPs would aggregate, resulting in a color change. Moreover, by virtue of the masking effect of ethylenediaminetetraacetic acid (EDTA), the nanosensor could readily discriminate organic mercury and inorganic mercury (Hg(2+)), and it is thus anticipated to shed some light on the colorimetric sensing of organic mercury. So, a direct, simple colorimetric assay for selective determination of Hg species was obtained, presenting high detectability, such as up to 10 nM for Hg(2+) and 15 nM for methylmercury. Meanwhile, the strategy offered excellent selectivity toward mercury species against other metal ions. The simple, rapid, and sensitive label-free colorimetric sensor for the determination of Hg species provided an attractive alternative to conventional methods, which usually involve sophisticated instruments, complicated processes, and long periods of time. More importantly, by using mercury as a model, an excellent nanomaterial-based optical sensing platform can be developed for speciation analysis of trace heavy metals, which can lead to nanomaterials stability change through smart functionalization and reasonable interactions.

  20. Colorimetric plasmon resonance microfluidics on nanohole array sensors

    Directory of Open Access Journals (Sweden)

    Austin Hsiao


    Full Text Available We present the label-free colorimetric visualization in microfluidics using plasmon resonance on a large-area and over a wide field-of-view (>100 mm2 nanohole array device called nanoLycurgus Cup Array (nanoLCA. We demonstrate the spectral detection and colorimetric sensing of static solutions of different concentrations of glycerol–water confined in parallel microfluidic channels integrated with nanoLCA. Taking advantage of the large sensor area and the colorimetric sensing capability of nanoLCA, we visualize in real-time the modulation of two immiscible solutions (water and oil, generated with integrated flow-focus microfluidics, in a label-free manner. Finite Element Method (FEM based simulation tool (COMSOL was used to verify the droplet formation in the microfluidics. Finite Difference Time Domain (FDTD electromagnetic simulation was used to identify the resonance modes of the plasmonic sensor. Finally, we demonstrate the real-time monitoring of streptavidin–biotin biomolecular interaction with the plasmonic biosensor.

  1. 罗氏生化多项定标液(CFAS)在 ADVIA1800自动生化仪上的应用%Roche biochemical multiple calibration solution (CFAS)used in the ADVIA 1800 automatic biochemical discussion on

    Institute of Scientific and Technical Information of China (English)



    目的:规范实验室生化项目的参考范围,统一临床检验数值,为临床医师提供更准确、更一致的检验结果,方便患者治疗效果的评价。方法同步于罗氏Cobas C501自动生化仪和ADVIA1800自动生化仪上进行多项目的评价。结果1.定标与校正验证合格;2.正常质控品和异常质控品在可控范围内;3.临床常规标本比对评价没有显著性差异。结论罗氏生化多项定标液(CFAS)在ADVIA1800自动生化仪上的应用效果满意,能为医师提供准确、统一的检验结果,方便医师治疗病人及疗效观察。%Objective to standardize laboratory biochemical reference scope of the project ,unified clinical inspection number , provide clinicians with more accurate and more consistent test results ,convenient patient treatment effect evaluation .Methods the synchronous in roche Cobas C501 automatic biochemistry analyzer and multiple objective evaluation ADVIA 1800 automatic bio‐chemistry analyzer .Results 1 ,the calibration and correction verification qualified ,2 ,normal quality products and quality control within the scope of the controllable ,3 ,there is no significant difference between clinical specimens of normal ratio evaluation .Con‐clusion roche biochemical multiple scaling liquid(CFAS)in the application effect of satisfaction on ADVIA 1800 automatic biochem‐istry analyzer ,for physicians to provide accurate ,unified inspection result ,convenient for doctors treat patients and curative effect observation .

  2. A new colorimetric breath indicator (Colibri). A comparison of the performance of two carbon dioxide indicators. (United States)

    Gedeon, A; Krill, P; Mebius, C


    Colorimetric carbon dioxide indicators are designed for the verification of satisfactory tracheal tube placement. However, widespread use of the device in this and other applications is hampered by limitations in its performance characteristics. A new colorimetric breath indicator (Colibri) has been developed that overcomes major shortcomings of current technology. A simple method is described that can be used to evaluate the properties of colorimetric carbon dioxide indicators in a quantitative way.

  3. Traceable Pyrgeometer Calibrations

    Energy Technology Data Exchange (ETDEWEB)

    Dooraghi, Mike; Kutchenreiter, Mark; Reda, Ibrahim; Habte, Aron; Sengupta, Manajit; Andreas, Afshin; Newman, Martina


    This poster presents the development, implementation, and operation of the Broadband Outdoor Radiometer Calibrations (BORCAL) Longwave (LW) system at the Southern Great Plains Radiometric Calibration Facility for the calibration of pyrgeometers that provide traceability to the World Infrared Standard Group.

  4. Calibration of sound calibrators: an overview (United States)

    Milhomem, T. A. B.; Soares, Z. M. D.


    This paper presents an overview of calibration of sound calibrators. Initially, traditional calibration methods are presented. Following, the international standard IEC 60942 is discussed emphasizing parameters, target measurement uncertainty and criteria for conformance to the requirements of the standard. Last, Regional Metrology Organizations comparisons are summarized.

  5. Bio-fabrication of gold nanoparticles using aqueous extract of red tomato and its use as a colorimetric sensor (United States)

    Barman, Gadadhar; Maiti, Swarnali; Laha, Jayasree Konar


    In this work, we report a green method for the synthesis of gold nanoparticles (GNP) using the aqueous extract of red tomato ( Lycopersicon esculentum). We believe that citric acid and ascorbic acid present in tomato juice are responsible for the reduction of gold ions. This biosynthesized GNP in the presence of sodium dodecyl sulfate has been used as a colorimetric sensor to detect and estimate the pesticide, methyl parathion. The GNP in the presence of methyl parathion shows a new peak at 400 nm due to the formation of 4-nitrophenolate ion by catalytic hydrolysis of methyl parathion in alkaline medium. A calibration curve between the absorption coefficients of the 400-nm peak versus the concentration of the pesticide allows the quantitative estimation of the 4-nitrophenolate ion, thereby enabling indirect estimation of methyl parathion present in the system.


    Directory of Open Access Journals (Sweden)

    A. M. G. Tommaselli


    Full Text Available The aim of this paper is to present results achieved with a 3D terrestrial calibration field, designed for calibrating digital cameras and omnidirectional sensors. This terrestrial calibration field is composed of 139 ARUCO coded targets. Some experiments were performed using a Nikon D3100 digital camera with 8mm Samyang Bower fisheye lens. The camera was calibrated in this terrestrial test field using a conventional bundle adjustment with the Collinearity and mathematical models specially designed for fisheye lenses. The CMC software (Calibration with Multiple Cameras, developed in-house, was used for the calibration trials. This software was modified to use fisheye models to which the Conrady-Brown distortion equations were added. The target identification and image measurements of its four corners were performed automatically with a public software. Several experiments were performed with 16 images and the results were presented and compared. Besides the calibration of fish-eye cameras, the field was designed for calibration of a catadrioptic system and brief informations on the calibration of this unit will be provided in the paper.

  7. A novel colorimetric method for field arsenic speciation analysis

    Institute of Scientific and Technical Information of China (English)

    Shan Hu; Jinsuo Lu; Chuanyong Jing


    Accurate on-site determination of arsenic (As) concentration as well as its speciation presents a great environmental challenge especially to developing countries.To meet the need of routine field monitoring,we developed a rapid colorimetric method with a wide dynamic detection range and high precision.The novel application of KMnO4 and CH4N2S as effective As(Ⅲ) oxidant and As(Ⅴ) reductant,respectively,in the formation of molybdenum blue complexes enabled the differentiation of As(Ⅲ) and As(Ⅴ).The detection limit of the method was 8 μg/L with a linear range (R2 =0.998) of four orders of magnitude in total As concentrations.The As speciation in groundwater samples determined with the colorimetric method in the field were consistent with the results using the high performance liquid chromatography atomic fluorescence spectrometry,as evidenced by a linear correlation in paired analysis with a slope of 0.9990-0.9997 (p < 0,0001,n =28).The recovery of 96%-116% for total As,85%-122% for As(Ⅲ),and 88%-127% for As(Ⅴ) were achieved for groundwater samples with a total As concentration range 100-800 μg/L,The colorimetric result showed that 3.61 g/L As(Ⅲ) existed as the only As species in a real industrial wastewater,which was in good agreement with the HPLC-AFS result of 3.56 g/L As(Ⅲ),No interference with the color development was observed in the presence of sulfate,phosphate,silicate,humic acid,and heavy metals from complex water matrix.This accurate,sensitive,and easy-to-use method is especially suitable for field As determination.

  8. Silicomolybdenum Blue Colorimetric Determination of Available Si in Calcareous Soils

    Institute of Scientific and Technical Information of China (English)



    This paper deals with the determination of available Si content in calcareous soils with Mo blue colorimetric analysis method.The experimental results showed that two acid reagents of sodium acetate buffering solution (pH 4) and 0.025 mol/L citric acid generally had a strong ability of extracting soil available Si,and theri soil filtrates were colorless,On the contrary,two alkaline extractants of 20 mg/mL sodium carbonate and 0.5 mol/L sodium bicarbonate only got a relevantly lower soil avaiable Si and theri extracts appeared deep color of organic and other pigments,which could be decolorized by adding certain amounts of P-free activated charcoal (about 0.1g/g soil),Int the procedure of Mo blue colorimetry,adding proper amount of oxalic acid and K(SbO)C4H4O6 could eliminate the interference of P to Si and Si to P,respectively.The concentration of acids was not the major factor suppressing the interferences between P and Si in the colorimetric analysis.In the medium of 0.2-0.8mole H×+/L sulfuric acid,the intensity of the silicomolybdate blue color did not vary with acidity,no matter whether(NH4)2 Fe(SO4)2 or ascorbic acid was used as reductive reagent.About 10 minutes was needed to from a fully blue complex at 20-25°C,After 30 minutes,the blue color would slowly change into light one,and hence,the colorimetric process should be finished within a short time.

  9. Passive Leak Detection Using Commercial Hydrogen Colorimetric Indicator

    Energy Technology Data Exchange (ETDEWEB)

    Hartmann, Kevin [National Renewable Energy Lab. (NREL), Golden, CO (United States); Buttner, William [National Renewable Energy Lab. (NREL), Golden, CO (United States); Burgess, Robert [National Renewable Energy Lab. (NREL), Golden, CO (United States); Rivkin, Carl [National Renewable Energy Lab. (NREL), Golden, CO (United States)


    Element One, Inc. (, a small business with in Boulder, CO, has been developing hydrogen detection technology based upon a highly selective colorimetric indicator. In its native state, the indicator pigment is a pale gray color, but becomes black upon exposure to hydrogen. The colorimetric change can be readily observed by the naked eye without the need for supplemental electronics or other hardware. Recently, the colorimetric indicator was integrated into a pliable, self-adhesive tape that can readily wrap around pneumatic fittings to serve as a hydrogen leak detector. A prototype version of the Element One indicator tape was tested within an NREL hydrogen system and successfully identified the unexpected presence of a small leak; a summary document for this case study is presented in Appendix 1. The tape was subsequently configured into 10-foot rolls as a product prototype that has just recently been commercialized and marketed under the tradename DetecTape(R). Figure 1 shows the commercial version of DetecTape along with an indicator sample in its native state and one that had been exposed to hydrogen. DetecTape is a self-adhesive silicone-based tape impregnated with a proprietary hydrogen-sensitive indicator based on transition metal oxides. A length of the tape can be cut from the roll and stretched by a factor of two or three times around a fitting. Due to the self-adhesive property of the tape, this provides a tight seal around the fitting. The seal is not hermetic, and is not intended to prevent the release of a leaking gas. However, a portion of the hydrogen leaking from a wrapped fitting will pass through the tape and react with the active indicator impregnated within the tape, thereby inducing blackening.

  10. Increased Automation in Stereo Camera Calibration Techniques

    Directory of Open Access Journals (Sweden)

    Brandi House


    Full Text Available Robotic vision has become a very popular field in recent years due to the numerous promising applications it may enhance. However, errors within the cameras and in their perception of their environment can cause applications in robotics to fail. To help correct these internal and external imperfections, stereo camera calibrations are performed. There are currently many accurate methods of camera calibration available; however, most or all of them are time consuming and labor intensive. This research seeks to automate the most labor intensive aspects of a popular calibration technique developed by Jean-Yves Bouguet. His process requires manual selection of the extreme corners of a checkerboard pattern. The modified process uses embedded LEDs in the checkerboard pattern to act as active fiducials. Images are captured of the checkerboard with the LEDs on and off in rapid succession. The difference of the two images automatically highlights the location of the four extreme corners, and these corner locations take the place of the manual selections. With this modification to the calibration routine, upwards of eighty mouse clicks are eliminated per stereo calibration. Preliminary test results indicate that accuracy is not substantially affected by the modified procedure. Improved automation to camera calibration procedures may finally penetrate the barriers to the use of calibration in practice.

  11. Colorimetric elastase sensor with peptide conjugated cellulose nanocrystals is interfaced to dialysis membranes (United States)

    Clinical detection of human neutrophil elastase (HNE) as point of care biomarker or in situ colorimetric adjuvant to chronic wound dressings presents potential advantages in the management of chronic wounds. A colorimetric approach to the detection of HNE using cotton cellulose nanocrystals (CCN) i...

  12. Cost Effective Paper-Based Colorimetric Microfluidic Devices and Mobile Phone Camera Readers for the Classroom (United States)

    Koesdjojo, Myra T.; Pengpumkiat, Sumate; Wu, Yuanyuan; Boonloed, Anukul; Huynh, Daniel; Remcho, Thomas P.; Remcho, Vincent T.


    We have developed a simple and direct method to fabricate paper-based microfluidic devices that can be used for a wide range of colorimetric assay applications. With these devices, assays can be performed within minutes to allow for quantitative colorimetric analysis by use of a widely accessible iPhone camera and an RGB color reader application…

  13. Model Calibration in Watershed Hydrology (United States)

    Yilmaz, Koray K.; Vrugt, Jasper A.; Gupta, Hoshin V.; Sorooshian, Soroosh


    Hydrologic models use relatively simple mathematical equations to conceptualize and aggregate the complex, spatially distributed, and highly interrelated water, energy, and vegetation processes in a watershed. A consequence of process aggregation is that the model parameters often do not represent directly measurable entities and must, therefore, be estimated using measurements of the system inputs and outputs. During this process, known as model calibration, the parameters are adjusted so that the behavior of the model approximates, as closely and consistently as possible, the observed response of the hydrologic system over some historical period of time. This Chapter reviews the current state-of-the-art of model calibration in watershed hydrology with special emphasis on our own contributions in the last few decades. We discuss the historical background that has led to current perspectives, and review different approaches for manual and automatic single- and multi-objective parameter estimation. In particular, we highlight the recent developments in the calibration of distributed hydrologic models using parameter dimensionality reduction sampling, parameter regularization and parallel computing.

  14. Unassisted 3D camera calibration (United States)

    Atanassov, Kalin; Ramachandra, Vikas; Nash, James; Goma, Sergio R.


    With the rapid growth of 3D technology, 3D image capture has become a critical part of the 3D feature set on mobile phones. 3D image quality is affected by the scene geometry as well as on-the-device processing. An automatic 3D system usually assumes known camera poses accomplished by factory calibration using a special chart. In real life settings, pose parameters estimated by factory calibration can be negatively impacted by movements of the lens barrel due to shaking, focusing, or camera drop. If any of these factors displaces the optical axes of either or both cameras, vertical disparity might exceed the maximum tolerable margin and the 3D user may experience eye strain or headaches. To make 3D capture more practical, one needs to consider unassisted (on arbitrary scenes) calibration. In this paper, we propose an algorithm that relies on detection and matching of keypoints between left and right images. Frames containing erroneous matches, along with frames with insufficiently rich keypoint constellations, are detected and discarded. Roll, pitch yaw , and scale differences between left and right frames are then estimated. The algorithm performance is evaluated in terms of the remaining vertical disparity as compared to the maximum tolerable vertical disparity.

  15. Object Detection and Tracking-Based Camera Calibration for Normalized Human Height Estimation

    Directory of Open Access Journals (Sweden)

    Jaehoon Jung


    Full Text Available This paper presents a normalized human height estimation algorithm using an uncalibrated camera. To estimate the normalized human height, the proposed algorithm detects a moving object and performs tracking-based automatic camera calibration. The proposed method consists of three steps: (i moving human detection and tracking, (ii automatic camera calibration, and (iii human height estimation and error correction. The proposed method automatically calibrates camera by detecting moving humans and estimates the human height using error correction. The proposed method can be applied to object-based video surveillance systems and digital forensic.

  16. Colorimetric Integrated PCR Protocol for Rapid Detection of Vibrio parahaemolyticus

    Directory of Open Access Journals (Sweden)

    Kewen Cheng


    Full Text Available Rapid detection of pathogens is of great significance for food safety and disease diagnosis. A new colorimetric method for rapid and easy detection of Vibrio parahaemolyticus (V. parahaemolyticus or Vp has been developed in this research. A specific sequence was designed and integrated with the forward primer for molecular detection of Vp. This specific sequence was tested and treated as the horseradish peroxidase (HRP-mimicking DNAzyme and could be amplified during the polymerase chain reaction (PCR process. The products of PCR including the sequence of HRP-mimicking DNAzyme could produce the distinguished color in the presence of catalysis substrates. The optical signal of the catalysis reaction, which is in a linear relationship with the initial template of Vp, could be determined with the naked eye or measured with Ultraviolet-visible (UV-vis for qualitative and quantitative detections, respectively. Based on the optical signal intensity, rapid and easy detection of Vp was successfully achieved with satisfied sensitivity and specificity. Furthermore, the detection of tdh, trh, tlh and toxR virulence genes of two Vp species (Vp 33847 and Vp 17802 were all performed successfully with this developed colorimetric integrated PCR protocol, which demonstrated potential applicability for the rapid detection of other bacteria.

  17. Multidimensional colorimetric sensor array for discrimination of proteins. (United States)

    Mao, Jinpeng; Lu, Yuexiang; Chang, Ning; Yang, Jiaoe; Zhang, Sichun; Liu, Yueying


    An extensible multidimensional colorimetric sensor array for the detection of protein is developed based on DNA functionalized gold nanoparticles (DNA-AuNPs) as receptors. In the presence of different proteins, the aggregation behavior of DNA-AuNPs was regulated by the high concentrations of salt and caused different color change; while DNA-AuNPs grew induced by the reduction of HAuCl4 and NH2OH as a reductant on the surface of nanoparticles exhibited different morphologies and color appearance for different proteins. The transducers based on AuNPs modified by specific and nonspecific DNA enables naked-eye discrimination of the target analytes. This extensible sensing platform with only two receptors could simultaneously discriminate ten native proteins and their thermally denatured conformations using hierarchical cluster analysis (HCA) at the concentration of 50nM with 100% accuracy. This opens up the possibility of the sensor array to investigate the different conformational changes of biomacromolecules, and it gives a new direction of developing multidimensional transduction principles based on plasmonic nanoparticle conjugates. Furthermore, the sensing system could discriminate proteins at the concentration of 500nM in the presence of 50% human urine, which indicated this sensor array has great potential ability in analyzing real biological fluids. In addition, the multidimensional colorimetric sensor array is suitable for analysis of target analytes in the resource-restricted regions because of rapid, simple, low cost, and in-field detection with the naked eye.

  18. Colorimetric Integrated PCR Protocol for Rapid Detection of Vibrio parahaemolyticus (United States)

    Cheng, Kewen; Pan, Daodong; Teng, Jun; Yao, Li; Ye, Yingwang; Xue, Feng; Xia, Fan; Chen, Wei


    Rapid detection of pathogens is of great significance for food safety and disease diagnosis. A new colorimetric method for rapid and easy detection of Vibrio parahaemolyticus (V. parahaemolyticus or Vp) has been developed in this research. A specific sequence was designed and integrated with the forward primer for molecular detection of Vp. This specific sequence was tested and treated as the horseradish peroxidase (HRP)-mimicking DNAzyme and could be amplified during the polymerase chain reaction (PCR) process. The products of PCR including the sequence of HRP-mimicking DNAzyme could produce the distinguished color in the presence of catalysis substrates. The optical signal of the catalysis reaction, which is in a linear relationship with the initial template of Vp, could be determined with the naked eye or measured with Ultraviolet-visible (UV-vis) for qualitative and quantitative detections, respectively. Based on the optical signal intensity, rapid and easy detection of Vp was successfully achieved with satisfied sensitivity and specificity. Furthermore, the detection of tdh, trh, tlh and toxR virulence genes of two Vp species (Vp 33847 and Vp 17802) were all performed successfully with this developed colorimetric integrated PCR protocol, which demonstrated potential applicability for the rapid detection of other bacteria. PMID:27690041

  19. Optical fiber waveguide sensor for the colorimetric detection of ammonia (United States)

    Schmitt, Katrin; Rist, Jonas; Peter, Carolin; Wöllenstein, Jürgen


    We present the development and characterization of a fiber-optic colorimetric gas sensor combined with the electronic circuitry for measurement control and RFID communication. The gas sensor detects ammonia using a 300 μm polyolefin fiber coated with a gas-sensitive polymer film. The spectral and time-dependent sensitivity of various polymer films was tested in transmission measurements. Light from a standard LED at λ = 590 nm was coupled into the polyolefin fiber through the front face. A prototype of the gas sensor with the direct coupling method was tested under realistic measurement conditions, i.e. battery-driven and in a completely autonomous mode. The sensor system showed good sensitivity to the ammonia concentrations and response times in the order of minutes. The achievable power consumption was below 100μW.The films contained the pH-sensitive dyes bromocresol purple or bromophenol blue embedded in either ethyl cellulose or polyvinyl butyral, and optionally tributyl phosphate as plasticizer. The bromophenol blue based films showed a strong reaction to ammonia, with saturation concentrations around 1000 ppm and response times of about 15 seconds to 100ppm. The colorimetric reaction was simulated using a simple kinetic model which was in good agreement with the experimental results.

  20. Charge Transfer Based Colorimetric Detection of Silver Ion

    Energy Technology Data Exchange (ETDEWEB)

    Han, Seung Choul; Kim, Kwang Seob; Choi, Soon Kyu; Oh, Jinho; Lee, Jae Wook [Dong-A Univ., Busan (Korea, Republic of)


    We have demonstrated the colorimetric chemosensor for detection of Ag{sup +} via formation of nanoparticles which is based on the intramolecular CT interaction between the electron-rich (2,6-dialkoxynaphthalene; Np) moiety and the electron-deficient (methyl viologen; MV{sup 2+}) moiety of a single sensor molecule. Under irradiation of light, Ag{sup +} was reduced to very small silver nanoparticle by CT interaction in the presence of OEGs as flexible recognition moiety of Ag{sup +} and stabilizer for Ag nanoparticles, thus Ag nanoparticles resulted to reddish brown in the color change of sensor solution, gradually. Therefore, the charge-transfer interaction between an electron-deficient and an electron-rich units existing at a sensor molecule can be regarded as a new and efficient method to construct various colorimetric chemosensors. Donor.acceptor interactions or charge transfer (CT) interactions are an important class of non-covalent interactions and have been widely exploited in self-assembling systems. Beyond molecular chemistry, supramolecular chemistry aims at constituting highly complex, functional chemical systems from components held together by intermolecular forces. Chemosensors are the molecules of abiotic origin that bind selectively and reversibly with the analyte with concomitant change in one or more properties of the system. The recognition and signaling of ionic and neutral species of varying complexity is one of the most intensively studied areas of contemporary supramolecular chemistry.

  1. Interfacial polymerization for colorimetric labeling of protein expression in cells.

    Directory of Open Access Journals (Sweden)

    Jacob L Lilly

    Full Text Available Determining the location of rare proteins in cells typically requires the use of on-sample amplification. Antibody based recognition and enzymatic amplification is used to produce large amounts of visible label at the site of protein expression, but these techniques suffer from the presence of nonspecific reactivity in the biological sample and from poor spatial control over the label. Polymerization based amplification is a recently developed alternative means of creating an on-sample amplification for fluorescence applications, while not suffering from endogenous labels or loss of signal localization. This manuscript builds upon polymerization based amplification by developing a stable, archivable, and colorimetric mode of amplification termed Polymer Dye Labeling. The basic concept involves an interfacial polymer grown at the site of protein expression and subsequent staining of this polymer with an appropriate dye. The dyes Evans Blue and eosin were initially investigated for colorimetric response in a microarray setting, where both specifically stained polymer films on glass. The process was translated to the staining of protein expression in human dermal fibroblast cells, and Polymer Dye Labeling was specific to regions consistent with desired protein expression. The labeling is stable for over 200 days in ambient conditions and is also compatible with modern mounting medium.

  2. Interfacial polymerization for colorimetric labeling of protein expression in cells. (United States)

    Lilly, Jacob L; Sheldon, Phillip R; Hoversten, Liv J; Romero, Gabriela; Balasubramaniam, Vivek; Berron, Brad J


    Determining the location of rare proteins in cells typically requires the use of on-sample amplification. Antibody based recognition and enzymatic amplification is used to produce large amounts of visible label at the site of protein expression, but these techniques suffer from the presence of nonspecific reactivity in the biological sample and from poor spatial control over the label. Polymerization based amplification is a recently developed alternative means of creating an on-sample amplification for fluorescence applications, while not suffering from endogenous labels or loss of signal localization. This manuscript builds upon polymerization based amplification by developing a stable, archivable, and colorimetric mode of amplification termed Polymer Dye Labeling. The basic concept involves an interfacial polymer grown at the site of protein expression and subsequent staining of this polymer with an appropriate dye. The dyes Evans Blue and eosin were initially investigated for colorimetric response in a microarray setting, where both specifically stained polymer films on glass. The process was translated to the staining of protein expression in human dermal fibroblast cells, and Polymer Dye Labeling was specific to regions consistent with desired protein expression. The labeling is stable for over 200 days in ambient conditions and is also compatible with modern mounting medium.

  3. Strategy for automatic and complete three-dimensional optical digitization



    This Letter proposes a new strategy of a three-dimensional (3D) scanning pipeline to achieve complete 3D digitization of complex objects in a real scene. This strategy consists of a one-dimensional array of optical 3D sensors combined with an automatically controlled turntable. An efficient calibration method for the sensor array is presented to guarantee the accuracy of the 3D measurement. Furthermore, an automatic registration technique is also proposed for aligning multiple range images ta...

  4. Trinocular Calibration Method Based on Binocular Calibration

    Directory of Open Access Journals (Sweden)

    CAO Dan-Dan


    Full Text Available In order to solve the self-occlusion problem in plane-based multi-camera calibration system and expand the measurement range, a tri-camera vision system based on binocular calibration is proposed. The three cameras are grouped into two pairs, while the public camera is taken as the reference to build the global coordinate. By calibration of the measured absolute distance and the true absolute distance, global calibration is realized. The MRE (mean relative error of the global calibration of the two camera pairs in the experiments can be as low as 0.277% and 0.328% respectively. Experiment results show that this method is feasible, simple and effective, and has high precision.

  5. Colorimetric determination of o-phenylenediamine in water samples based on the formation of silver nanoparticles as a colorimetric probe. (United States)

    Li, Nan; Gu, Yu; Gao, Mengmeng; Wang, Zilu; Xiao, Deli; Li, Yun; Lin, Rui; He, Hua


    A simple, rapid and cost-effective method for visual colorimetric detection of o-phenylenediamine (OPD) based on the formation of silver nanoparticles (AgNPs) has been developed in this paper. Silver ions can be reduced to AgNPs by OPD in a few minutes, causing changes in absorption spectra and color of the reaction system. Therefore, colorimetric detection of OPD could be realized by a UV-vis spectrophotometer or even the naked eye. Results showed that the absorption intensity of AgNPs at 416 nm exhibited a good linear correlation (R2=0.998) with OPD concentration in the range from 10(-6) to 8×10(-5) mol L(-1) and the detection limit (3σ/S) was calculated to be 1.61×10(-7) mol L(-1). Furthermore, as low as 4×10(-6) mol L(-1)OPD can be visualized by the naked eye without the requirement of any complicated or expensive instruments. This proposed method has been successfully applied to determine OPD in water samples, and may provide an innovative platform in the development of sensors for guiding environmental monitoring in the future.


    Numerical models are a useful tool in evaluating and designing NAPL remediation systems. Traditional constitutive finite difference and finite element models are complex and expensive to apply. For this reason, this paper presents the application of a simplified stochastic-Lagran...

  7. Automatic Fiscal Stabilizers

    Directory of Open Access Journals (Sweden)

    Narcis Eduard Mitu


    Full Text Available Policies or institutions (built into an economic system that automatically tend to dampen economic cycle fluctuations in income, employment, etc., without direct government intervention. For example, in boom times, progressive income tax automatically reduces money supply as incomes and spendings rise. Similarly, in recessionary times, payment of unemployment benefits injects more money in the system and stimulates demand. Also called automatic stabilizers or built-in stabilizers.

  8. Automatic differentiation bibliography

    Energy Technology Data Exchange (ETDEWEB)

    Corliss, G.F. (comp.)


    This is a bibliography of work related to automatic differentiation. Automatic differentiation is a technique for the fast, accurate propagation of derivative values using the chain rule. It is neither symbolic nor numeric. Automatic differentiation is a fundamental tool for scientific computation, with applications in optimization, nonlinear equations, nonlinear least squares approximation, stiff ordinary differential equation, partial differential equations, continuation methods, and sensitivity analysis. This report is an updated version of the bibliography which originally appeared in Automatic Differentiation of Algorithms: Theory, Implementation, and Application.

  9. Colorimetric Sensor Arrays System Based on FPGA for Image Recognition

    Institute of Scientific and Technical Information of China (English)

    Rui Chen; Jian-Hua Xu; Ya-Dong Jiang


    A FPGA-based image recognition system is designed for colorimetric sensor array in order to recognize a wide range of volatile organic compounds. The gas molecule is detected by the responsive sensor array and the responsive image is obtained. The image is decomposed to RGB color components using CMOS image sensor. An embedded image recognition archi- tecture based on Xilinx Spartan-3 FPGA is designed to implement the algorithms of image recognition. The algorithm of color coherence vector is discussed in detail[X1] compared with the algorithm of color histograms, and experimental results demonstrate that both of the two algorithms could be analyzed effectively to represent different volatile organic compounds according to their different responsive images in this system.

  10. Hybrid nanosensor for colorimetric and ultrasensitive detection of nuclease contaminations (United States)

    Cecere, Paola; Valentini, Paola; Pompa, Pier Paolo


    Nucleases are ubiquitous enzymes that degrade DNA or RNA, thus they can prejudice the good outcome of molecular biology experiments involving nucleic acids. We propose a colorimetric test for the naked-eye detection of nuclease contaminations. The system uses an hybrid nanosensor, based on gold nanoparticles functionalized with DNA probes. Our assay is rapid, instrument-free, simple and low-cost. Moreover, it reaches sensitivity equal or better than those of commercial kits, and presents a lot of advantageous aspects. Therefore, it is very competitive, with a real market potential. This test will be relevant in routine process monitoring in scientific laboratories, and in quality control in clinical laboratories and industrial processes, allowing the simultaneous detection of nucleases with different substrate specificities and large-scale screening.

  11. Colorimetric studies of some newly synthesized bisazo reactive dyes

    Directory of Open Access Journals (Sweden)

    Divyesh R. Patel


    Full Text Available A series of cold brand bisazo reactive dyes (4a–h were obtained by the coupling of tetrazotised 4,4′-methylene-bis(2-methyl-5-nitro aniline (2 with various cyanurated coupling components (3a–h in good yield. Their dyeing performances as reactive dyes have been assessed on silk, wool and cotton fabrics. These dyes were characterized by UV–Vis, FTIR, 1H NMR spectroscopic techniques elemental analysis. The percentage dye bath exhaustion and fixation on different fibers were found to be very good. The dyed fabric showed moderate to very good light fastness and good to excellent washing and rubbing fastness properties. Spectral properties and colorimetric data (L∗, a∗, b∗, C∗, H∗, K/S have also been studied in detail.

  12. The calibration of PIXIE (United States)

    Fixsen, D. J.; Chuss, D. T.; Kogut, Alan; Mirel, Paul; Wollack, E. J.


    The FIRAS instrument demonstrated the use of an external calibrator to compare the sky to an instrumented blackbody. The PIXIE calibrator is improved from -35 dB to -65 dB. Another significant improvement is the ability to insert the calibrator into either input of the FTS. This allows detection and correction of additional errors, reduces the effective calibration noise by a factor of 2, eliminates an entire class of systematics and allows continuous observations. This paper presents the design and use of the PIXIE calibrator.

  13. Mediation and Automatization. (United States)

    Hutchins, Edwin

    This paper discusses the relationship between the mediation of task performance by some structure that is not inherent in the task domain itself and the phenomenon of automatization, in which skilled performance becomes effortless or phenomenologically "automatic" after extensive practice. The use of a common simple explicit mediating…

  14. Digital automatic gain control (United States)

    Uzdy, Z.


    Performance analysis, used to evaluated fitness of several circuits to digital automatic gain control (AGC), indicates that digital integrator employing coherent amplitude detector (CAD) is best device suited for application. Circuit reduces gain error to half that of conventional analog AGC while making it possible to automatically modify response of receiver to match incoming signal conditions.

  15. Automatic Differentiation Package

    Energy Technology Data Exchange (ETDEWEB)


    Sacado is an automatic differentiation package for C++ codes using operator overloading and C++ templating. Sacado provide forward, reverse, and Taylor polynomial automatic differentiation classes and utilities for incorporating these classes into C++ codes. Users can compute derivatives of computations arising in engineering and scientific applications, including nonlinear equation solving, time integration, sensitivity analysis, stability analysis, optimization and uncertainity quantification.

  16. Development and validation of a colorimetric sensor array for fish spoilage monitoring

    DEFF Research Database (Denmark)

    Morsy, Mohamed K.; Zor, Kinga; Kostesha, Natalie;


    Given the need for non-destructive methods and sensors for food spoilage monitoring, we have evaluated sixteen chemo-sensitive compounds incorporated in an array for colorimetric detection of typical spoilage compounds (trimethylamine, dimethylamine, cadaverine, putrescine) and characterized...

  17. Direct quantification of carotenoids in low fat babyfoods via laser photoacoustics and colorimetric index a

    NARCIS (Netherlands)

    Doka, O.; Ajtony, Z.; Bicanic, D.D.; Valinger, D.; Vegvari, G.


    Carotenoids are important antioxidants found in various foods including those for nutrition of infants. In this investigation, the total carotenoid content (TCC) of nine different commercially available baby foods was quantified using colorimetric index a * obtained via reflectance colorimetry (RC)

  18. Operator-Friendly Technique and Quality Control Considerations for Indigo Colorimetric Measurement of Ozone Residual (United States)

    Drinking water ozone disinfection systems measure ozone residual concentration, C, for regulatory compliance reporting of concentration-times-time (CT), and the resultant log-inactivation of virus, Giardia and Cryptosporidium. The indigotrisulfonate (ITS) colorimetric procedure i...

  19. Distributed Radio Interferometric Calibration

    CERN Document Server

    Yatawatta, Sarod


    Increasing data volumes delivered by a new generation of radio interferometers require computationally efficient and robust calibration algorithms. In this paper, we propose distributed calibration as a way of improving both computational cost as well as robustness in calibration. We exploit the data parallelism across frequency that is inherent in radio astronomical observations that are recorded as multiple channels at different frequencies. Moreover, we also exploit the smoothness of the variation of calibration parameters across frequency. Data parallelism enables us to distribute the computing load across a network of compute agents. Smoothness in frequency enables us reformulate calibration as a consensus optimization problem. With this formulation, we enable flow of information between compute agents calibrating data at different frequencies, without actually passing the data, and thereby improving robustness. We present simulation results to show the feasibility as well as the advantages of distribute...

  20. The Science of Calibration (United States)

    Kent, S. M.


    This paper presents a broad overview of the many issues involved in calibrating astronomical data, covering the full electromagnetic spectrum from radio waves to gamma rays, and considering both ground-based and space-based missions. These issues include the science drivers for absolute and relative calibration, the physics behind calibration and the mechanisms used to transfer it from the laboratory to an astronomical source, the need for networks of calibrated astronomical standards, and some of the challenges faced by large surveys and missions.

  1. Towards automatic classification of all WISE sources

    CERN Document Server

    Kurcz, Agnieszka; Solarz, Aleksandra; Krupa, Magdalena; Pollo, Agnieszka; Małek, Katarzyna


    The WISE satellite has detected hundreds of millions sources over the entire sky. Classifying them reliably is however a challenging task due to degeneracies in WISE multicolour space and low levels of detection in its two longest-wavelength bandpasses. Here we aim at obtaining comprehensive and reliable star, galaxy and quasar catalogues based on automatic source classification in full-sky WISE data. This means that the final classification will employ only parameters available from WISE itself, in particular those reliably measured for a majority of sources. For the automatic classification we applied the support vector machines (SVM) algorithm, which requires a training sample with relevant classes already identified, and we chose to use the SDSS spectroscopic dataset for that purpose. By calibrating the classifier on the test data drawn from SDSS, we first established that a polynomial kernel is preferred over a radial one for this particular dataset. Next, using three classification parameters (W1 magnit...

  2. Quercetin as colorimetric reagent for determination of zirconium (United States)

    Grimaldi, F.S.; White, C.E.


    Methods described in the literature for the determination of zirconium are generally designed for relatively large amounts of this element. A good procedure using colorimetric reagent for the determination of trace amounts is desirable. Quercetin has been found to yield a sensitive color reaction with zirconium suitable for the determination of from 0.1 to 50?? of zirconium dioxide. The procedure developed involves the separation of zirconium from interfering elements by precipitation with p-dimethylaminoazophenylarsonic acid prior to its estimation with quercetin. The quercetin reaction is carried out in 0.5N hydrochloric acid solution. Under the operating conditions it is indicated that quercetin forms a 2 to 1 complex with zirconium; however, a 2 to 1 and a 1 to 1 complex can coexist under special conditions. Approximate values for the equilibrium constants of the complexes are K1 = 0.33 ?? 10-5 and K2 = 1.3 ?? 10-9. Seven Bureau of Standards samples of glass sands and refractories were analyzed with excellent results. The method described should find considerable application in the analysis of minerals and other materials for macro as well as micro amounts of zirconium.

  3. Colorimetric biosensing of targeted gene sequence using dual nanoparticle platforms

    Directory of Open Access Journals (Sweden)

    Thavanathan J


    Full Text Available Jeevan Thavanathan,1 Nay Ming Huang,1 Kwai Lin Thong2 1Low Dimension Material Research Center, Department of Physics, 2Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia Abstract: We have developed a colorimetric biosensor using a dual platform of gold nanoparticles and graphene oxide sheets for the detection of Salmonella enterica. The presence of the invA gene in S. enterica causes a change in color of the biosensor from its original pinkish-red to a light purplish solution. This occurs through the aggregation of the primary gold nanoparticles–conjugated DNA probe onto the surface of the secondary graphene oxide–conjugated DNA probe through DNA hybridization with the targeted DNA sequence. Spectrophotometry analysis showed a shift in wavelength from 525 nm to 600 nm with 1 µM of DNA target. Specificity testing revealed that the biosensor was able to detect various serovars of the S. enterica while no color change was observed with the other bacterial species. Sensitivity testing revealed the limit of detection was at 1 nM of DNA target. This proves the effectiveness of the biosensor in the detection of S. enterica through DNA hybridization. Keywords: biosensor, DNA hybridization, DNA probe, gold nanoparticles, graphene oxide, Salmonella enterica

  4. Word Automaticity of Tree Automatic Scattered Linear Orderings Is Decidable

    CERN Document Server

    Huschenbett, Martin


    A tree automatic structure is a structure whose domain can be encoded by a regular tree language such that each relation is recognisable by a finite automaton processing tuples of trees synchronously. Words can be regarded as specific simple trees and a structure is word automatic if it is encodable using only these trees. The question naturally arises whether a given tree automatic structure is already word automatic. We prove that this problem is decidable for tree automatic scattered linear orderings. Moreover, we show that in case of a positive answer a word automatic presentation is computable from the tree automatic presentation.

  5. OLI Radiometric Calibration (United States)

    Markham, Brian; Morfitt, Ron; Kvaran, Geir; Biggar, Stuart; Leisso, Nathan; Czapla-Myers, Jeff


    Goals: (1) Present an overview of the pre-launch radiance, reflectance & uniformity calibration of the Operational Land Imager (OLI) (1a) Transfer to orbit/heliostat (1b) Linearity (2) Discuss on-orbit plans for radiance, reflectance and uniformity calibration of the OLI

  6. Lidar to lidar calibration

    DEFF Research Database (Denmark)

    Fernandez Garcia, Sergio; Villanueva, Héctor

    This report presents the result of the lidar to lidar calibration performed for ground-based lidar. Calibration is here understood as the establishment of a relation between the reference lidar wind speed measurements with measurement uncertainties provided by measurement standard and correspondi...

  7. Automatic classification of blank substrate defects (United States)

    Boettiger, Tom; Buck, Peter; Paninjath, Sankaranarayanan; Pereira, Mark; Ronald, Rob; Rost, Dan; Samir, Bhamidipati


    Mask preparation stages are crucial in mask manufacturing, since this mask is to later act as a template for considerable number of dies on wafer. Defects on the initial blank substrate, and subsequent cleaned and coated substrates, can have a profound impact on the usability of the finished mask. This emphasizes the need for early and accurate identification of blank substrate defects and the risk they pose to the patterned reticle. While Automatic Defect Classification (ADC) is a well-developed technology for inspection and analysis of defects on patterned wafers and masks in the semiconductors industry, ADC for mask blanks is still in the early stages of adoption and development. Calibre ADC is a powerful analysis tool for fast, accurate, consistent and automatic classification of defects on mask blanks. Accurate, automated classification of mask blanks leads to better usability of blanks by enabling defect avoidance technologies during mask writing. Detailed information on blank defects can help to select appropriate job-decks to be written on the mask by defect avoidance tools [1][4][5]. Smart algorithms separate critical defects from the potentially large number of non-critical defects or false defects detected at various stages during mask blank preparation. Mechanisms used by Calibre ADC to identify and characterize defects include defect location and size, signal polarity (dark, bright) in both transmitted and reflected review images, distinguishing defect signals from background noise in defect images. The Calibre ADC engine then uses a decision tree to translate this information into a defect classification code. Using this automated process improves classification accuracy, repeatability and speed, while avoiding the subjectivity of human judgment compared to the alternative of manual defect classification by trained personnel [2]. This paper focuses on the results from the evaluation of Automatic Defect Classification (ADC) product at MP Mask

  8. Sandia WIPP calibration traceability

    Energy Technology Data Exchange (ETDEWEB)

    Schuhen, M.D. [Sandia National Labs., Albuquerque, NM (United States); Dean, T.A. [RE/SPEC, Inc., Albuquerque, NM (United States)


    This report summarizes the work performed to establish calibration traceability for the instrumentation used by Sandia National Laboratories at the Waste Isolation Pilot Plant (WIPP) during testing from 1980-1985. Identifying the calibration traceability is an important part of establishing a pedigree for the data and is part of the qualification of existing data. In general, the requirement states that the calibration of Measuring and Test equipment must have a valid relationship to nationally recognized standards or the basis for the calibration must be documented. Sandia recognized that just establishing calibration traceability would not necessarily mean that all QA requirements were met during the certification of test instrumentation. To address this concern, the assessment was expanded to include various activities.

  9. WFPC2 Polarization Calibration (United States)

    Biretta, J.; McMaster, M.


    We derive a detailed calibration for WFPC2 polarization data which is accurate to about 1.5%. We begin by computing polarizer flats, and show how they are applied to data. A physical model for the polarization effects of the WFPC2 optics is then created using Mueller matricies. This model includes corrections for the instrumental polarization (diattenuation and phase retardance) of the pick-off mirror, as well as the high cross-polarization transmission of the polarizer filter. We compare this model against the on-orbit observations of polarization calibrators, and show it predicts relative counts in the different polarizer/aperture settings to 1.5% RMS accuracy. We then show how this model can be used to calibrate GO data, and present two WWW tools which allow observers to easily calibrate their data. Detailed examples are given illustrationg the calibration and display of WFPC2 polarization data. In closing we describe future plans and possible improvements.

  10. A reliable and reproducible method for the lipase assay in an AOT/isooctane reversed micellar system: modification of the copper-soap colorimetric method. (United States)

    Kwon, Chang Woo; Park, Kyung-Min; Choi, Seung Jun; Chang, Pahn-Shick


    The copper-soap method, which is based on the absorbance of a fatty acid-copper complex at 715 nm, is a widely used colorimetric assay to determine the lipase activity in reversed micellar system. However, the absorbance of the bis(2-ethylhexyl) sodium sulfosuccinate (AOT)-copper complex prevents the use of an AOT/isooctane reversed micellar system. An extraction step was added to the original procedure to remove AOT and eliminate interference from the AOT-copper complex. Among the solvents tested, acetonitrile was determined to be the most suitable because it allows for the generation of a reproducible calibration curve with oleic acid that is independent of the AOT concentrations. Based on the validation data, the modified method, which does not experience interference from the AOT-copper complex, could be a useful method with enhanced accuracy and reproducibility for the lipase assay.

  11. Comparison of colorimetric assays with quantitative amino acid analysis for protein quantification of Generalized Modules for Membrane Antigens (GMMA). (United States)

    Rossi, Omar; Maggiore, Luana; Necchi, Francesca; Koeberling, Oliver; MacLennan, Calman A; Saul, Allan; Gerke, Christiane


    Genetically induced outer membrane particles from Gram-negative bacteria, called Generalized Modules for Membrane Antigens (GMMA), are being investigated as vaccines. Rapid methods are required for estimating the protein content for in-process assays during production. Since GMMA are complex biological structures containing lipid and polysaccharide as well as protein, protein determinations are not necessarily straightforward. We compared protein quantification by Bradford, Lowry, and Non-Interfering assays using bovine serum albumin (BSA) as standard with quantitative amino acid (AA) analysis, the most accurate currently available method for protein quantification. The Lowry assay has the lowest inter- and intra-assay variation and gives the best linearity between protein amount and absorbance. In all three assays, the color yield (optical density per mass of protein) of GMMA was markedly different from that of BSA with a ratio of approximately 4 for the Bradford assay, and highly variable between different GMMA; and approximately 0.7 for the Lowry and Non-Interfering assays, highlighting the need for calibrating the standard used in the colorimetric assay against GMMA quantified by AA analysis. In terms of a combination of ease, reproducibility, and proportionality of protein measurement, and comparability between samples, the Lowry assay was superior to Bradford and Non-Interfering assays for GMMA quantification.

  12. Comparison of rapid colorimetric method with conventional method in the isolation of mycobacterium tuberculosis

    Directory of Open Access Journals (Sweden)

    Oberoi A


    Full Text Available The aim of the study was to evaluate two methods (colorimetric and conventional for isolation of Mycobacterium tuberculosis. A total of 500 clinical specimens were processed by modified Petroff′s method and then inoculated into MB/BacT-240 system bottles and on LJ medium slopes. The specimens included 242 sputum, 95 gastric aspirates, 47 pleural fluids, 45 CSF, 32 urine, 18 pus, 11 bronchoalveolar lavage, 3 tissue, 2 stool, 2 lymphnode specimens, 2 synovial fluid and 1 bronchial wash specimens. The isolation rate was 16.4% by the colorimetric method and 2.2% by the conventional method. The mean detection time was 16 days and 26 days respectively. Among 36 direct smear positive samples, 63.9%(23/36 and 30%(11/36 were positive by colorimetric and conventional methods respectively. Out of 464 direct smear negative samples 12.9%(60/464 and 0.6%(3/464 were positive by colorimetric and conventional methods respectively. Therefore, colorimetric method enables rapid detection leading to early diagnosis and drug susceptibility testing.

  13. Rapid colorimetric sensing of tetracycline antibiotics with in situ growth of gold nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Shen, Li, E-mail: [Logistics School, Beijing Wuzi University, Beijing 101149 (China); Chen, Jing; Li, Na [Logistics School, Beijing Wuzi University, Beijing 101149 (China); He, Pingli [State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100094 (China); Li, Zhen [State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193 (China)


    Highlights: • Tetracyclines directly reduce aurate into gold nanoparticles. • Gold nanoparticles showed characteristic plamson absorbance at 526 nm. • Quantitative detection of tetracyclines with the colorimetric assay. • Tetracyclines spiked urine samples can be detected with the assay. - Abstract: A colorimetric assay utilizing the formation of gold nanoparticles was developed to detect tetracycline antibiotics in fluidic samples. Tetracycline antibiotics showed the capability of directly reducing aurate salts into atomic gold which form gold nanoparticles spontaneously under proper conditions. The resulted gold nanoparticles showed characteristic plasmon absorbance at 526 nm, which can be visualized by naked eyes or with a spectrophotometer. UV–vis absorbance of the resulted gold nanoparticles is correlated directly with the concentrations of tetracycline antibiotics in the solution, allowing for quantitative colorimetric detection of tetracycline antibiotics. Reaction conditions, such as pH, temperature, reaction time, and ionic strength were optimized. Sensitivity of the colorimetric assay can be enhanced by the addition of gold nanoparticle seeds, a LOD as low as 20 ng mL{sup −1} can be achieved with the help of seed particles. The colorimetric assay showed minimum interference from ethanol, methanol, urea, glucose, and other antibiotics such as sulfonamides, amino glycosides etc. Validity of the method was also evaluated on urine samples spiked with tetracycline antibiotics. The method provides a broad spectrum detection method for rapid and sensitive detection of reductive substances such as tetracycline antibiotics in liquid and biological samples.

  14. Segment Based Camera Calibration

    Institute of Scientific and Technical Information of China (English)

    马颂德; 魏国庆; 等


    The basic idea of calibrating a camera system in previous approaches is to determine camera parmeters by using a set of known 3D points as calibration reference.In this paper,we present a method of camera calibration in whih camera parameters are determined by a set of 3D lines.A set of constraints is derived on camea parameters in terms of perspective line mapping.Form these constraints,the same perspective transformation matrix as that for point mapping can be computed linearly.The minimum number of calibration lines is 6.This result generalizes that of Liu,Huang and Faugeras[12] for camera location determination in which at least 8 line correspondences are required for linear computation of camera location.Since line segments in an image can be located easily and more accurately than points,the use of lines as calibration reference tends to ease the computation in inage preprocessing and to improve calibration accuracy.Experimental results on the calibration along with stereo reconstruction are reported.

  15. Lidar to lidar calibration

    DEFF Research Database (Denmark)

    Georgieva Yankova, Ginka; Courtney, Michael

    This report presents the result of the lidar to lidar calibration performed for ground-based lidar. Calibration is here understood as the establishment of a relation between the reference lidar wind speed measurements with measurement uncertainties provided by measurement standard and corresponding...... lidar wind speed indications with associated measurement uncertainties. The lidar calibration concerns the 10 minute mean wind speed measurements. The comparison of the lidar measurements of the wind direction with that from the reference lidar measurements are given for information only....

  16. Site Calibration report

    DEFF Research Database (Denmark)

    Gómez Arranz, Paula; Vesth, Allan

    This report describes the site calibration carried out at Østerild, during a given period. The site calibration was performed with two Windcube WLS7 (v1) lidars at ten measurements heights. The lidar is not a sensor approved by the current version of the IEC 61400-12-1 [1] and therefore the site...... calibration with lidars does not comply with the standard. However, the measurements are carried out following the guidelines of IEC 61400-12-1 where possible, but with some deviations presented in the following chapters....

  17. Spectroscopic Analysis of Organophosphorus Pesticides Using Colorimetric Reactions

    Institute of Scientific and Technical Information of China (English)

    Amit Kumar Sharma


    The UV-Vis and infrared (IR)absorption spectra of organophosphorus (OP)pesticides have been studied.A correlation in spectra was developed to optimize the OP pesticides in the environments.The spec-troscopic (UV-Vis and IR)spectrum of OP pesticides like methyl parathion,malathion and parathion has been interpreted in detail.A complete calculation of the normal frequencies and absolute intensities of UV-Vis and IR absorption bands are interpreted with the help of corresponding experimental data.In the colorimetric reac-tions,the bands appear at 2 077,1 637,1 455,1 015,655 cm-1 for malathion;2 081,1 639,1 316,1 015, 794,683 cm-1 for parathion;2 078,1 632,1 032,794 cm-1 for methyl parathion were used for quantitative or qualitative analysis.All these IR spectra were acquired by averaging 100 scans at a resolution of 4 cm-1 .It is determined experimentally in the region 200~450 nm for UV-Vis absorption bands and in the region 400~4 000 cm-1 for IR absorption bands.It is concluded that the mainly optically active groups (P—OH, C—O, P—O, C—O—C,P—O—C, P—S, —OH)present in pesticides which are responsible in change in sig-nificant data for quantitative and qualitative analysis.The various optical properties like wavelength,band en-ergy,wave number,and frequency,also are calculated.

  18. Calibration Fixture For Anemometer Probes (United States)

    Lewis, Charles R.; Nagel, Robert T.


    Fixture facilitates calibration of three-dimensional sideflow thermal anemometer probes. With fixture, probe oriented at number of angles throughout its design range. Readings calibrated as function of orientation in airflow. Calibration repeatable and verifiable.

  19. Automatic Program Development

    DEFF Research Database (Denmark)

    by members of the IFIP Working Group 2.1 of which Bob was an active member. All papers are related to some of the research interests of Bob and, in particular, to the transformational development of programs and their algorithmic derivation from formal specifications. Automatic Program Development offers......Automatic Program Development is a tribute to Robert Paige (1947-1999), our accomplished and respected colleague, and moreover our good friend, whose untimely passing was a loss to our academic and research community. We have collected the revised, updated versions of the papers published in his...... honor in the Higher-Order and Symbolic Computation Journal in the years 2003 and 2005. Among them there are two papers by Bob: (i) a retrospective view of his research lines, and (ii) a proposal for future studies in the area of the automatic program derivation. The book also includes some papers...

  20. Simple flow injection colorimetric system for determination of paraquat in natural water. (United States)

    Chuntib, Prakit; Jakmunee, Jaroon


    A simple and low cost flow injection colorimetric system has been developed for determination of paraquat in natural water. The developed method is based on the reduction of paraquat by using sodium dithionite as a reducing agent in an alkaline medium to produce a blue free radical ion that can be detected by a simple light emitting diode-light dependent resistor (LED-LDR) colorimeter. The standard or sample solution was injected via a set of 3-way solenoid valves into a water carrier stream and flowed to merge with reagent to generate a colored product which is proportional to the concentration of paraquat ion in the solution. Under the optimum condition of the system, i.e., mixing coil length 30 cm, flow rate 2.0 mL min(-1), sample volume 100 μL, concentrations of dithionite 0.1% (w/v) and sodium hydroxide 0.06 mol L(-1), a linear calibration graph in the range of 0.2-10.0 mg L(-1) with a correlation coefficient of 0.9996, and a limit of detection of 0.15 mg L(-1) were achieved. Relative standard deviation for 9 replicate injections of 1 mg L(-1) paraquat is 3.7%. A sample throughput of 40 injections h(-1) was achieved. The limit of detection can be improved by off-line preconcentration of paraquat employing a column packed with Dowex 50WX8-100 (H) cation exchange resin and eluted with 10% (w/v) ammonium chloride in ammonium buffer solution pH 10. The eluting solution was then injected into the FI system for paraquat determination. The proposed system did not suffer from interferences of some possible ions in natural water and other herbicides. Recoveries obtained by spiking 0.5 and 5.0 mg L(-1) paraquat standard into water samples were in the range of 104-110% and 101-105%, respectively. The developed system can be conveniently applied for screening of paraquat contaminated in natural water.

  1. SPOTS Calibration Example

    Directory of Open Access Journals (Sweden)

    Patterson E.


    Full Text Available The results are presented using the procedure outlined by the Standardisation Project for Optical Techniques of Strain measurement to calibrate a digital image correlation system. The process involves comparing the experimental data obtained with the optical measurement system to the theoretical values for a specially designed specimen. The standard states the criteria which must be met in order to achieve successful calibration, in addition to quantifying the measurement uncertainty in the system. The system was evaluated at three different displacement load levels, generating strain ranges from 289 µstrain to 2110 µstrain. At the 289 µstrain range, the calibration uncertainty was found to be 14.1 µstrain, and at the 2110 µstrain range it was found to be 28.9 µstrain. This calibration procedure was performed without painting a speckle pattern on the surface of the metal. Instead, the specimen surface was prepared using different grades of grit paper to produce the desired texture.

  2. Air Data Calibration Facility (United States)

    Federal Laboratory Consortium — This facility is for low altitude subsonic altimeter system calibrations of air vehicles. Mission is a direct support of the AFFTC mission. Postflight data merge is...

  3. Traceable Pyrgeometer Calibrations

    Energy Technology Data Exchange (ETDEWEB)

    Dooraghi, Mike; Kutchenreiter, Mark; Reda, Ibrahim; Habte, Aron; Sengupta, Manajit; Andreas, Afshin; Newman, Martina; Webb, Craig


    This presentation provides a high-level overview of the progress on the Broadband Outdoor Radiometer Calibrations for all shortwave and longwave radiometers that are deployed by the Atmospheric Radiation Measurement program.

  4. SRHA calibration curve (United States)

    U.S. Environmental Protection Agency — an UV calibration curve for SRHA quantitation This dataset is associated with the following publication: Chang, X., and D. Bouchard. Surfactant-Wrapped Multiwalled...

  5. Calibrating nacelle lidars

    DEFF Research Database (Denmark)

    Courtney, Michael

    Nacelle mounted, forward looking wind lidars are beginning to be used to provide reference wind speed measurements for the power performance testing of wind turbines. In such applications, a formal calibration procedure with a corresponding uncertainty assessment will be necessary. This report...... presents four concepts for performing such a nacelle lidar calibration. Of the four methods, two are found to be immediately relevant and are pursued in some detail. The first of these is a line of sight calibration method in which both lines of sight (for a two beam lidar) are individually calibrated...... a representative distribution of radial wind speeds. An alternative method is to place the nacelle lidar on the ground and incline the beams upwards to bisect a mast equipped with reference instrumentation at a known height and range. This method will be easier and faster to implement and execute but the beam...

  6. Automatic text summarization

    CERN Document Server

    Torres Moreno, Juan Manuel


    This new textbook examines the motivations and the different algorithms for automatic document summarization (ADS). We performed a recent state of the art. The book shows the main problems of ADS, difficulties and the solutions provided by the community. It presents recent advances in ADS, as well as current applications and trends. The approaches are statistical, linguistic and symbolic. Several exemples are included in order to clarify the theoretical concepts.  The books currently available in the area of Automatic Document Summarization are not recent. Powerful algorithms have been develop

  7. Automatic Camera Control

    DEFF Research Database (Denmark)

    Burelli, Paolo; Preuss, Mike


    Automatically generating computer animations is a challenging and complex problem with applications in games and film production. In this paper, we investigate howto translate a shot list for a virtual scene into a series of virtual camera configurations — i.e automatically controlling the virtual...... camera. We approach this problem by modelling it as a dynamic multi-objective optimisation problem and show how this metaphor allows a much richer expressiveness than a classical single objective approach. Finally, we showcase the application of a multi-objective evolutionary algorithm to generate a shot...

  8. Colorimetric characterization of digital cameras with unrestricted capture settings applicable for different illumination circumstances (United States)

    Fang, Jingyu; Xu, Haisong; Wang, Zhehong; Wu, Xiaomin


    With colorimetric characterization, digital cameras can be used as image-based tristimulus colorimeters for color communication. In order to overcome the restriction of fixed capture settings adopted in the conventional colorimetric characterization procedures, a novel method was proposed considering capture settings. The method calculating colorimetric value of the measured image contains five main steps, including conversion from RGB values to equivalent ones of training settings through factors based on imaging system model so as to build the bridge between different settings, scaling factors involved in preparation steps for transformation mapping to avoid errors resulted from nonlinearity of polynomial mapping for different ranges of illumination levels. The experiment results indicate that the prediction error of the proposed method, which was measured by CIELAB color difference formula, reaches less than 2 CIELAB units under different illumination levels and different correlated color temperatures. This prediction accuracy for different capture settings remains the same level as the conventional method for particular lighting condition.

  9. Label-free colorimetric estimation of proteins using nanoparticles of silver

    Institute of Scientific and Technical Information of China (English)

    Siddhartha Shrivastava; Debabrata Dash


    Metallic nanoparticles have received considerable attention in bioassays and diagnostics due to their unique surface plasmon resonance (SPR) properties. Gold nanoparticles have been employed for the development of SPR-based colorimetric bioassays. In the present report we have described a sensitive colorimetric approach for estimation of proteins, within a detection limit of 10~80 μg/mL, using unmodified silver nanoparticles. Besides the common advantages of colorimetric assay such as simplicity, high sensitivity, and low cost, our method has a label-free design and provides an important and attractive alternative to classical sensing probes and systems. The present work will contribute to the development of nanotechnology-based diagnostic tools.

  10. 4-mercaptophenylboronic acid functionalized gold nanoparticles for colorimetric sialic acid detection. (United States)

    Sankoh, Supannee; Thammakhet, Chongdee; Numnuam, Apon; Limbut, Warakorn; Kanatharana, Proespichaya; Thavarungkul, Panote


    A simple and selective colorimetric sensor for sialic acid detection, based on the aggregation of 4-mercaptophenylboronic acid functionalized gold nanoparticles (4-MPBA-AuNPs) was developed. The color of the solution changed from wine-red to blue after binding with sialic acid. The colorimetric sensor provided good analytical performances with a linear dynamic range of 80µM to 2.00mM and a 68±2µM limit of detection without any effect from possible interferences and sample matrix. In addition, the quantitative results were obtained within only 10min. This developed sensor was used to detect sialic acid in blood serum samples and the results were in good agreement with those from the current periodate-resorcinol method (P>0.05) thus indicating that this developed colorimetric sensor can be used as an alternative method for sialic acid detection with a shorter analysis time and a high accuracy.

  11. Performance of automatic scanning microscope for nuclear emulsion experiments

    Energy Technology Data Exchange (ETDEWEB)

    Güler, A. Murat, E-mail: [Middle East Technical University, 06800 Ankara (Turkey); Altınok, Özgür [Middle East Technical University, 06800 Ankara (Turkey); Tufts University, Medford, MA 02155 (United States)


    The impressive improvements in scanning technology and methods let nuclear emulsion to be used as a target in recent large experiments. We report the performance of an automatic scanning microscope for nuclear emulsion experiments. After successful calibration and alignment of the system, we have reached 99% tracking efficiency for the minimum ionizing tracks that penetrating through the emulsions films. The automatic scanning system is successfully used for the scanning of emulsion films in the OPERA experiment and plan to use for the next generation of nuclear emulsion experiments.

  12. Calibrating nacelle lidars


    Courtney, Michael


    Nacelle mounted, forward looking wind lidars are beginning to be used to provide reference wind speed measurements for the power performance testing of wind turbines. In such applications, a formal calibration procedure with a corresponding uncertainty assessment will be necessary. This report presents four concepts for performing such a nacelle lidar calibration. Of the four methods, two are found to be immediately relevant and are pursued in some detail.The first of these is a line of sight...

  13. TWSTFT Link Calibration Report (United States)


    box calibrator with unknown but constant total delay during a calibration tour Total Delay: The total electrical delay from the antenna phase the UTCp including all the devices/cables that the satellite and clock signals pass through. It numerically equals the sum of all the sub-delays...PTB. To average out the dimnal effects and measurement noise , 5-7 days of continuous measurements is required. 3 Setups at the Lab(k) The setup

  14. Calibrating nacelle lidars

    Energy Technology Data Exchange (ETDEWEB)

    Courtney, M.


    Nacelle mounted, forward looking wind lidars are beginning to be used to provide reference wind speed measurements for the power performance testing of wind turbines. In such applications, a formal calibration procedure with a corresponding uncertainty assessment will be necessary. This report presents four concepts for performing such a nacelle lidar calibration. Of the four methods, two are found to be immediately relevant and are pursued in some detail. The first of these is a line of sight calibration method in which both lines of sight (for a two beam lidar) are individually calibrated by accurately aligning the beam to pass close to a reference wind speed sensor. A testing procedure is presented, reporting requirements outlined and the uncertainty of the method analysed. It is seen that the main limitation of the line of sight calibration method is the time required to obtain a representative distribution of radial wind speeds. An alternative method is to place the nacelle lidar on the ground and incline the beams upwards to bisect a mast equipped with reference instrumentation at a known height and range. This method will be easier and faster to implement and execute but the beam inclination introduces extra uncertainties. A procedure for conducting such a calibration is presented and initial indications of the uncertainties given. A discussion of the merits and weaknesses of the two methods is given together with some proposals for the next important steps to be taken in this work. (Author)

  15. Energy calibration via correlation

    CERN Document Server

    Maier, Daniel


    The main task of an energy calibration is to find a relation between pulse-height values and the corresponding energies. Doing this for each pulse-height channel individually requires an elaborated input spectrum with an excellent counting statistics and a sophisticated data analysis. This work presents an easy to handle energy calibration process which can operate reliably on calibration measurements with low counting statistics. The method uses a parameter based model for the energy calibration and concludes on the optimal parameters of the model by finding the best correlation between the measured pulse-height spectrum and multiple synthetic pulse-height spectra which are constructed with different sets of calibration parameters. A CdTe-based semiconductor detector and the line emissions of an 241 Am source were used to test the performance of the correlation method in terms of systematic calibration errors for different counting statistics. Up to energies of 60 keV systematic errors were measured to be le...

  16. Lysine-promoted colorimetric response of gold nanoparticles: a simple assay for ultrasensitive mercury(II) detection. (United States)

    Sener, Gulsu; Uzun, Lokman; Denizli, Adil


    Although numerous methods have been reported for the analysis of toxic mercury (Hg(2+)) ions in drinking water, development of simple, rapid, inexpensive, and sensitive sensors still remains a great challenge. Here, we report a simple yet very sensitive colorimetric assay for rapid detection of Hg(2+) in water. The colorimetric assay is based on the aggregation of as-prepared citrate-capped gold nanoparticles (AuNPs) in the presence of Hg(2+) ions and the positively charged amino acid, lysine. The detection limit of this inexpensive colorimetric assay is 2.9 nM, which is below the limit value (10 nM) defined by the U.S. Environmental Protection Agency in drinkable water. Also, the colorimetric response of citrate-capped AuNPs in the presence of lysine is very selective to the Hg(2+). In addition, the colorimetric assay is very fast, and all analyses can be completed within a few minutes.

  17. Enzyme-free colorimetric detection systems based on the DNA strand displacement competition reaction

    DEFF Research Database (Denmark)

    Zhang, Zhao; Birkedal, Victoria; Gothelf, Kurt Vesterager


    The strand displacement competition assay is based on the dynamic equilibrium of the competitive hybridization of two oligonucleotides (A and B) to a third oligonucleotide (S). In the presence of an analyte that binds to a specific affinity-moiety conjugated to strand B, the equilibrium shifts, w...... G-quadruplex DNAzyme for colorimetric readout of the detection of streptavidin by the naked eye. Finally, we integrate the whole G-quadruplex DNAzyme system in a single DNA strand and show that it is applicable to colorimetric detection....

  18. Development of an Ultrasensitive ELISA-Bienzyme Colorimetric Substrate Recycle Assay for Measurement of Tau

    Institute of Scientific and Technical Information of China (English)

    DUAN Qiu-hong; WANG Xiao-chuan; WANG Xi-ming; ZHOU Xin-wen; HE Shan-shu; WANG Jian-zhi


    On the basis of conventional enzyme-linked immunosorbent assay (ELISA) and bienzyme substrate recycle, ELISA-bienzyme colorimetric substrate recycle was developed in the present study. The sensitivity of this method increased 15 times than that of ELISA for the measurement of Tau and increased 55 times for p-Tau. The linear detective rang of this method expanded 3 times higher than that of conventional ELISA for Tau and had the same as ELISA for p-Tau. So, ELISA-bienzyme colorimetric substrate recycle could be used to detect abnormally phosphorylated tau in cerebrospinal fluid.

  19. Relationship between colorimetric (instrumental) evaluation and consumer-defined beef colour acceptability. (United States)

    Holman, Benjamin W B; Mao, Yanwei; Coombs, Cassius E O; van de Ven, Remy J; Hopkins, David L


    The relationship between instrumental colorimetric values (L*, a*, b*, the ratio of reflectance at 630nm and 580nm) and consumer perception of acceptable beef colour was evaluated using a web-based survey and standardised photographs of beef m. longissimus lumborum with known colorimetrics. Only L* and b* were found to relate to average consumer opinions of beef colour acceptability. Respondent nationality was also identified as a source of variation in beef colour acceptability score. Although this is a preliminary study with the findings necessitating additional investigation, these results suggest L* and b* as candidates for developing instrumental thresholds for consumer beef colour expectations.

  20. Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles

    Directory of Open Access Journals (Sweden)

    Yunxia Luan


    Full Text Available A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA was developed using a cationic polymer and gold nanoparticles (AuNPs. The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by the cationic polymer. By spectroscopic quantitative analysis, the colorimetric assay could detect OTA down to 0.009 ng/mL with high selectivity in the presence of other interfering toxins. This study offers a new alternative in visual detection methods that is rapid and sensitive for OTA detection.

  1. Simple, field portable colorimetric detection device for organic peroxides and hydrogen peroxide (United States)

    Pagoria, Philip F.; Mitchell, Alexander R.; Whipple, Richard E.; Carman, M. Leslie; Reynolds, John G.; Nunes, Peter; Shields, Sharon J.


    A simple and effective system for the colorimetric determination of organic peroxides and hydrogen peroxide. A peroxide pen utilizing a swipe material attached to a polyethylene tube contains two crushable vials. The two crushable vials contain a colorimetric reagent separated into dry ingredients and liquid ingredients. After swiping a suspected substance or surface the vials are broken, the reagent is mixed thoroughly and the reagent is allowed to wick into the swipe material. The presence of organic peroxides or hydrogen peroxide is confirmed by a deep blue color.

  2. Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles. (United States)

    Luan, Yunxia; Chen, Jiayi; Li, Cheng; Xie, Gang; Fu, Hailong; Ma, Zhihong; Lu, Anxiang


    A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by the cationic polymer. By spectroscopic quantitative analysis, the colorimetric assay could detect OTA down to 0.009 ng/mL with high selectivity in the presence of other interfering toxins. This study offers a new alternative in visual detection methods that is rapid and sensitive for OTA detection.

  3. Automatic Complexity Analysis

    DEFF Research Database (Denmark)

    Rosendahl, Mads


    One way to analyse programs is to to derive expressions for their computational behaviour. A time bound function (or worst-case complexity) gives an upper bound for the computation time as a function of the size of input. We describe a system to derive such time bounds automatically using abstract...

  4. Exploring Automatization Processes. (United States)

    DeKeyser, Robert M.


    Presents the rationale for and the results of a pilot study attempting to document in detail how automatization takes place as the result of different kinds of intensive practice. Results show that reaction times and error rates gradually decline with practice, and the practice effect is skill-specific. (36 references) (CK)

  5. RGB Color Calibration for Quantitative Image Analysis: The “3D Thin-Plate Spline” Warping Approach

    Directory of Open Access Journals (Sweden)

    Corrado Costa


    Full Text Available In the last years the need to numerically define color by its coordinates in n-dimensional space has increased strongly. Colorimetric calibration is fundamental in food processing and other biological disciplines to quantitatively compare samples’ color during workflow with many devices. Several software programmes are available to perform standardized colorimetric procedures, but they are often too imprecise for scientific purposes. In this study, we applied the Thin-Plate Spline interpolation algorithm to calibrate colours in sRGB space (the corresponding Matlab code is reported in the Appendix. This was compared with other two approaches. The first is based on a commercial calibration system (ProfileMaker and the second on a Partial Least Square analysis. Moreover, to explore device variability and resolution two different cameras were adopted and for each sensor, three consecutive pictures were acquired under four different light conditions. According to our results, the Thin-Plate Spline approach reported a very high efficiency of calibration allowing the possibility to create a revolution in the in-field applicative context of colour quantification not only in food sciences, but also in other biological disciplines. These results are of great importance for scientific color evaluation when lighting conditions are not controlled. Moreover, it allows the use of low cost instruments while still returning scientifically sound quantitative data.

  6. Detection of biological thiols based on a colorimetric method

    Institute of Scientific and Technical Information of China (English)

    Yuan-yuan XU; Yang-yang SUN; Yu-juan ZHANG; Chen-he LU; Jin-feng MIAO‡


    Biological thiols (biothiols), an important kind of functional biomolecules, such as cysteine (Cys) and glutathione (GSH), play vital roles in maintaining the stability of the intracellular environment. In past decades, studies have demonstrated that metabolic disorder of biothiols is related to many serious disease processes and wil lead to extreme damage in human and numerous animals. We carried out a series of experiments to detect biothiols in bi-osamples, including bovine plasma and cel lysates of seven different cel lines based on a simple colorimetric method. In a typical test, the color of the test solution could gradualy change from blue to colorless after the addition of biothiols. Based on the color change displayed, experimental results reveal that the percentage of biothiols in the embryonic fibroblast cell line is significantly higher than those in the other six cell lines, which provides the basis for the following biothiols-related study.%中文概要题目:生物巯化物的可视化检测目的:通过简单可靠的可视化检测方法评估牛血清及各细胞系中生物巯化物的含量。创新点:基于银纳米颗粒形成的比色变化过程对牛血清及细胞中生物巯化物进行了检测。方法:将6组不同的细胞系培养后进行裂解,其裂解产物分别与3,3',5,5'-四甲基联苯胺(TMB)和硝酸银(AgNO3)的混合液室温孵育后,用紫外可见分光光度计测量细胞中生物巯化物的含量。结论:通过不同细胞系中生物巯化物含量的比对,证实胚胎成纤维细胞中生物巯化物的含量明显高于其他细胞。

  7. Automaticity and Reading: Perspectives from the Instance Theory of Automatization. (United States)

    Logan, Gordon D.


    Reviews recent literature on automaticity, defining the criteria that distinguish automatic processing from non-automatic processing, and describing modern theories of the underlying mechanisms. Focuses on evidence from studies of reading and draws implications from theory and data for practical issues in teaching reading. Suggests that…

  8. Novel real-time alignment and calibration of the LHCb detector in Run II

    CERN Document Server

    Xu, Zhirui


    An automatic real-time alignment and calibration strategy of the LHCb detector was developed for the Run II. Thanks to the online calibration, tighter event selection criteria can be used in the trigger. Furthermore, the online calibration facilitates the use of hadronic particle identification using the Ring Imaging Cherenkov (RICH) detectors at the trigger level. The motivation for a real-time alignment and calibration of the LHCb detector is discussed from both the operational and physics performance points of view. Specific challenges of this novel configuration are discussed, as well as the working procedures of the framework and its performance.

  9. Gemini Planet Imager Calibrations, Pipeline Updates, and Campaign Data Processing (United States)

    Perrin, Marshall D.; Follette, Katherine B.; Millar-Blanchaer, Max; Wang, Jason; Wolff, Schulyer; Hung, Li-Wei; Arriaga, Pauline; Savransky, Dmitry; Bailey, Vanessa P.; Bruzzone, Sebastian; Chilcote, Jeffrey K.; De Rosa, Robert J.; Draper, Zachary; Fitzgerald, Michael P.; Greenbaum, Alexandra; Ingraham, Patrick; Konopacky, Quinn M.; Macintosh, Bruce; Marchis, Franck; Marois, Christian; Maire, Jerome; Nielsen, Eric L.; Rajan, Abhijith; Rameau, Julien; Rantakyro, Fredrik; Ruffio, Jean-Baptise; Tran, Debby; Ward-Duong, Kimberly; Zalesky, Joe; GPIES Team


    In support of GPI imaging and spectroscopy of exoplanets, polarimetry of disks, and the ongoing Exoplanet Survey we continue to refine calibrations, improve data reduction methods, and develop other enhancements to the data pipeline. We summarize here the latest updates to the open-source GPI Data Reduction Pipeline, including recent improvements spectroscopic and photometric calibrations and polarimetric data processing. For the GPI Exoplanet Survey we have incorporated the GPI Data Pipeline into a larger campaign data system that provides automatic data processing including rapid PSF subtraction and contrast measurements in real time during observations and fully automated PSF subtractions using several state-of-the-art algorithms shortly after each observation completes.

  10. Calibration Under Uncertainty.

    Energy Technology Data Exchange (ETDEWEB)

    Swiler, Laura Painton; Trucano, Timothy Guy


    This report is a white paper summarizing the literature and different approaches to the problem of calibrating computer model parameters in the face of model uncertainty. Model calibration is often formulated as finding the parameters that minimize the squared difference between the model-computed data (the predicted data) and the actual experimental data. This approach does not allow for explicit treatment of uncertainty or error in the model itself: the model is considered the %22true%22 deterministic representation of reality. While this approach does have utility, it is far from an accurate mathematical treatment of the true model calibration problem in which both the computed data and experimental data have error bars. This year, we examined methods to perform calibration accounting for the error in both the computer model and the data, as well as improving our understanding of its meaning for model predictability. We call this approach Calibration under Uncertainty (CUU). This talk presents our current thinking on CUU. We outline some current approaches in the literature, and discuss the Bayesian approach to CUU in detail.

  11. HAWC Timing Calibration

    CERN Document Server

    Huentemeyer, Petra; Dingus, Brenda


    The High-Altitude Water Cherenkov (HAWC) Experiment is a second-generation highsensitivity gamma-ray and cosmic-ray detector that builds on the experience and technology of the Milagro observatory. Like Milagro, HAWC utilizes the water Cherenkov technique to measure extensive air showers. Instead of a pond filled with water (as in Milagro) an array of closely packed water tanks is used. The event direction will be reconstructed using the times when the PMTs in each tank are triggered. Therefore, the timing calibration will be crucial for reaching an angular resolution as low as 0.25 degrees.We propose to use a laser calibration system, patterned after the calibration system in Milagro. Like Milagro, the HAWC optical calibration system will use ~1 ns laser light pulses. Unlike Milagro, the PMTs are optically isolated and require their own optical fiber calibration. For HAWC the laser light pulses will be directed through a series of optical fan-outs and fibers to illuminate the PMTs in approximately one half o...

  12. All-optical automatic pollen identification: Towards an operational system (United States)

    Crouzy, Benoît; Stella, Michelle; Konzelmann, Thomas; Calpini, Bertrand; Clot, Bernard


    We present results from the development and validation campaign of an optical pollen monitoring method based on time-resolved scattering and fluorescence. Focus is first set on supervised learning algorithms for pollen-taxa identification and on the determination of aerosol properties (particle size and shape). The identification capability provides a basis for a pre-operational automatic pollen season monitoring performed in parallel to manual reference measurements (Hirst-type volumetric samplers). Airborne concentrations obtained from the automatic system are compatible with those from the manual method regarding total pollen and the automatic device provides real-time data reliably (one week interruption over five months). In addition, although the calibration dataset still needs to be completed, we are able to follow the grass pollen season. The high sampling from the automatic device allows to go beyond the commonly-presented daily values and we obtain statistically significant hourly concentrations. Finally, we discuss remaining challenges for obtaining an operational automatic monitoring system and how the generic validation environment developed for the present campaign could be used for further tests of automatic pollen monitoring devices.

  13. Influence of rainfall observation network on model calibration and application

    Directory of Open Access Journals (Sweden)

    A. Bárdossy


    Full Text Available The objective in this study is to investigate the influence of the spatial resolution of the rainfall input on the model calibration and application. The analysis is carried out by varying the distribution of the raingauge network. A meso-scale catchment located in southwest Germany has been selected for this study. First, the semi-distributed HBV model is calibrated with the precipitation interpolated from the available observed rainfall of the different raingauge networks. An automatic calibration method based on the combinatorial optimization algorithm simulated annealing is applied. The performance of the hydrological model is analyzed as a function of the raingauge density. Secondly, the calibrated model is validated using interpolated precipitation from the same raingauge density used for the calibration as well as interpolated precipitation based on networks of reduced and increased raingauge density. Lastly, the effect of missing rainfall data is investigated by using a multiple linear regression approach for filling in the missing measurements. The model, calibrated with the complete set of observed data, is then run in the validation period using the above described precipitation field. The simulated hydrographs obtained in the above described three sets of experiments are analyzed through the comparisons of the computed Nash-Sutcliffe coefficient and several goodness-of-fit indexes. The results show that the model using different raingauge networks might need re-calibration of the model parameters, specifically model calibrated on relatively sparse precipitation information might perform well on dense precipitation information while model calibrated on dense precipitation information fails on sparse precipitation information. Also, the model calibrated with the complete set of observed precipitation and run with incomplete observed data associated with the data estimated using multiple linear regressions, at the locations treated as

  14. Calibration Systems Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Myers, Tanya L.; Broocks, Bryan T.; Phillips, Mark C.


    The Calibration Systems project at Pacific Northwest National Laboratory (PNNL) is aimed towards developing and demonstrating compact Quantum Cascade (QC) laser-based calibration systems for infrared imaging systems. These on-board systems will improve the calibration technology for passive sensors, which enable stand-off detection for the proliferation or use of weapons of mass destruction, by replacing on-board blackbodies with QC laser-based systems. This alternative technology can minimize the impact on instrument size and weight while improving the quality of instruments for a variety of missions. The potential of replacing flight blackbodies is made feasible by the high output, stability, and repeatability of the QC laser spectral radiance.

  15. Ibis ground calibration

    Energy Technology Data Exchange (ETDEWEB)

    Bird, A.J.; Barlow, E.J.; Tikkanen, T. [Southampton Univ., School of Physics and Astronomy (United Kingdom); Bazzano, A.; Del Santo, M.; Ubertini, P. [Istituto di Astrofisica Spaziale e Fisica Cosmica - IASF/CNR, Roma (Italy); Blondel, C.; Laurent, P.; Lebrun, F. [CEA Saclay - Sap, 91 - Gif sur Yvette (France); Di Cocco, G.; Malaguti, E. [Istituto di Astrofisica Spaziale e Fisica-Bologna - IASF/CNR (Italy); Gabriele, M.; La Rosa, G.; Segreto, A. [Istituto di Astrofisica Spaziale e Fisica- IASF/CNR, Palermo (Italy); Quadrini, E. [Istituto di Astrofisica Spaziale e Fisica-Cosmica, EASF/CNR, Milano (Italy); Volkmer, R. [Institut fur Astronomie und Astrophysik, Tubingen (Germany)


    We present an overview of results obtained from IBIS ground calibrations. The spectral and spatial characteristics of the detector planes and surrounding passive materials have been determined through a series of calibration campaigns. Measurements of pixel gain, energy resolution, detection uniformity, efficiency and imaging capability are presented. The key results obtained from the ground calibration have been: - optimization of the instrument tunable parameters, - determination of energy linearity for all detection modes, - determination of energy resolution as a function of energy through the range 20 keV - 3 MeV, - demonstration of imaging capability in each mode, - measurement of intrinsic detector non-uniformity and understanding of the effects of passive materials surrounding the detector plane, and - discovery (and closure) of various leakage paths through the passive shielding system.

  16. Colorimetric Detection of Creatinine Based on Plasmonic Nanoparticles via Synergistic Coordination Chemistry. (United States)

    Du, Jianjun; Zhu, Bowen; Leow, Wan Ru; Chen, Shi; Sum, Tze Chien; Peng, Xiaojun; Chen, Xiaodong


    A simple and portable colorimetric assay for creatinine detection is fabricated based on the synergistic coordination of creatinine and uric acid with Hg(2+) on the surface of gold nanoparticles, which exhibits good selectivity and sensitivity. Point-of-care clinical creatinine monitoring can be supported for monitoring renal function and diagnosing corresponding renal diseases at home.

  17. Zinc Biosorption by Seaweed Illustrated by the Zincon Colorimetric Method and the Langmuir Isotherm (United States)

    Areco, Maria Mar; dos Santos Afonso, Maria; Valdman, Erika


    An experiment is conducted to promote biotechnology knowledge that is an emerging technology on cleaning treatment, showing the potential of seaweed to remove heavy-metal ions from solution. The rapid and accurate determination of zinc in aqueous solution by the zincon colorimetric method gives an interesting and simple experiment for any…

  18. A Novel Colorimetric Immunoassay Utilizing the Peroxidase Mimicking Activity of Magnetic Nanoparticles

    Directory of Open Access Journals (Sweden)

    Hyun Gyu Park


    Full Text Available A simple colorimetric immunoassay system, based on the peroxidase mimicking activity of Fe3O4 magnetic nanoparticles (MNPs, has been developed to detect clinically important antigenic molecules. MNPs with ca. 10 nm in diameter were synthesized and conjugated with specific antibodies against target molecules, such as rotaviruses and breast cancer cells. Conjugation of the MNPs with antibodies (MNP-Abs enabled specific recognition of the corresponding target antigenic molecules through the generation of color signals arising from the colorimetric reaction between the selected peroxidase substrate, 3,3',5,5'-tetramethylbenzidine (TMB and H2O2. Based on the MNP-promoted colorimetric reaction, the target molecules were detected and quantified by measuring absorbance intensities corresponding to the oxidized form of TMB. Owing to the higher stabilities and economic feasibilities of MNPs as compared to horseradish peroxidase (HRP, the new colorimetric system employing MNP-Abs has the potential of serving as a potent immunoassay that should substitute for conventional HRP-based immunoassays. The strategy employed to develop the new methodology has the potential of being extended to the construction of simple diagnostic systems for a variety of biomolecules related to human cancers and infectious diseases, particularly in the realm of point-of-care applications.

  19. Microtiter plate based colorimetric assay for characterization of dehalogenation activity of GAC/Fe0 composite

    DEFF Research Database (Denmark)

    Hwang, Yuhoon; Salatas, Apostolos; Mines, Paul D.


    of nZVI and its composite with granular activated carbon (GAC). The assay focused on analysis of reaction products rather than its mother compounds, which gives more accurate quantification of reductive activity. The colorimetric assays were developed to quantify three reaction products, ammonia...

  20. A highly sensitive gold nanoparticle-based colorimetric probe for pyrophosphate using a competition assay approach. (United States)

    Kim, Sudeok; Eom, Min Sik; Kim, Seung Kyung; Seo, Seong Hyeok; Han, Min Su


    In this study, a mixture of [Zn(2)(1,3-bis[bis(2-pyridylmethyl)aminomethyl]benzene)](4+) ([Zn(2)(BBPAB)](4+)) and 11-mercaptoundecylphosphoric acid functionalized gold nanoparticles (Phos-AuNPs) is shown to be a highly sensitive colorimetric probe that can easily detect pyrophosphate (PPi) at less than 200 nM with the naked eye.

  1. Colorimetric assay for the detection of typical biomarkers for periodontitis using a magnetic nanoparticle biosensor

    NARCIS (Netherlands)

    Wignarajah, S.; Suaifan, G.A.R.Y.; Bizzarro, S.; Bikker, F.J.; Kaman, W.E.; Zourob, M.


    Periodontitis is a chronic disease which affects at least 10% of the population. If untreated, periodontitis can lead to teeth loss. Unfortunately, current diagnostic tests are limited in their sensitivity and specificity. In this study, a novel multiplex hand-held colorimetric diagnostic biosensor,

  2. High-efficiency phenotyping for vitamin A in banana using artificial neural networks and colorimetric data

    Directory of Open Access Journals (Sweden)

    César Fernandes Aquino


    Full Text Available ABSTRACT Banana is one of the most consumed fruits in Brazil and an important source of minerals, vitamins and carbohydrates for human diet. The characterization of banana superior genotypes allows identifying those with nutritional quality for cultivation and to integrate genetic improvement programs. However, identification and quantification of the provitamin carotenoids are hampered by the instruments and reagents cost for chemical analyzes, and it may become unworkable if the number of samples to be analyzed is high. Thus, the objective was to verify the potential of indirect phenotyping of the vitamin A content in banana through artificial neural networks (ANNs using colorimetric data. Fifteen banana cultivars with four replications were evaluated, totaling 60 samples. For each sample, colorimetric data were obtained and the vitamin A content was estimated in the ripe banana pulp. For the prediction of the vitamin A content by colorimetric data, multilayer perceptron ANNs were used. Ten network architectures were tested with a single hidden layer. The network selected by the best fit (least mean square error had four neurons in the hidden layer, enabling high efficiency in prediction of vitamin A (r2 = 0.98. The colorimetric parameters a* and Hue angle were the most important in this study. High-scale indirect phenotyping of vitamin A by ANNs on banana pulp is possible and feasible.

  3. Colorimetric Detection of Escherichia coli Based on the Enzyme-Induced Metallization of Gold Nanorods. (United States)

    Chen, Juhong; Jackson, Angelyca A; Rotello, Vincent M; Nugen, Sam R


    A novel enzyme-induced metallization colorimetric assay is developed to monitor and measure beta-galactosidase (β-gal) activity, and is further employed for colorimetric bacteriophage (phage)-enabled detection of Escherichia coli (E. coli). This assay relies on enzymatic reaction-induced silver deposition on the surface of gold nanorods (AuNRs). In the presence of β-gal, the substrate p-aminophenyl β-d-galactopyranoside is hydrolyzed to produce p-aminophenol (PAP). Reduction of silver ions by PAP generates a silver shell on the surface of AuNRs, resulting in the blue shift of the longitudinal localized surface plasmon resonance peak and multicolor changes of the detection solution from light green to orange-red. Under optimized conditions, the detection limit for β-gal is 128 pM, which is lower than the conventional colorimetric assay. Additionally, the assay has a broader dynamic range for β-gal detection. The specificity of this assay for the detection of β-gal is demonstrated against several protein competitors. Additionally, this technique is successfully applied to detect E. coli bacteria cells in combination with bacteriophage infection. Due to the simplicity and short incubation time of this enzyme-induced metallization colorimetric method, the assay is well suited for the detection of bacteria in low-resource settings.

  4. Potassium sorbate residue levels and persistence in citrus fruit as detected by a simple colorimetric method (United States)

    Postharvest applications of potassium sorbate (PS) to fresh citrus fruit control fungal decay pathogens, such as Penicillium digitatum, cause of green mold. Although PS effectiveness has been examined repeatedly, little is known about PS residues. A colorimetric method that employed extraction of th...

  5. An organic indicator functionalized graphene oxide nanocomposite-based colorimetric assay for the detection of sarcosine. (United States)

    Xue, Zhonghua; Yin, Bo; Wang, Hui; Li, Mengqian; Rao, Honghong; Liu, Xiuhui; Zhou, Xinbin; Lu, Xiaoquan


    Rapid detection of sarcosine is a key requirement for both diagnosis and treatment of disease. We report here a simple yet sensitive colorimetric nanocomposite platform for rapid detection of sarcosine in alkaline media. The approach exploited the benefits of a rapid color-producing reaction between an organic indicator, 1,2-naphthoquinone-4-sulphonic acid sodium salt (NQS), and the analyte of sarcosine species as well as the good catalytic ability of graphene oxide (GO) to the formation of highly colored products due to its good water dispersibility, extremely large surface area and facile surface modification. As a result, a NQS functionalized GO nanocomposite through π-π stacking has been demonstrated to be useful as a highly efficient catalyst system for the selective and sensitive colorimetric determination of sarcosine by providing a nanocomposite-amplified colorimetric response. Meanwhile, the strategy offered excellent selectivity toward sarcosine species against other amino acids as well as a satisfying detection limit of 0.73 μM. More importantly, by using an electrochemical method, a credible sensing mechanism of GO nanocomposite-based colorimetric platform for a special analyte determination can be easily verified and elucidated, which also provides an attractive alternative to conventional characterization strategies.

  6. Development of the colorimetric sensor array for detection of explosives and volatile organic compounds in air

    DEFF Research Database (Denmark)

    Kostesha, Natalie; Alstrøm, Tommy Sonne; Johnsen, C;


    In the framework of the research project 'Xsense' at the Technical University of Denmark (DTU) we are developing a simple colorimetric sensor array which can be useful in detection of explosives like DNT and TNT, and identification of volatile organic compounds in the presence of water vapor in air...

  7. Multi-colorimetric sensor array for detection of explosives in gas and liquid phase

    DEFF Research Database (Denmark)

    Kostesha, Natalie; Alstrøm, Tommy Sonne; Johnsen, C.;


    In the framework of the research project "Xsense" at the Technical University of Denmark (DTU) we are developing a simple colorimetric sensor array which can be useful in detection of explosives like DNT, TATP, HMX, RDX and identification of reagents needed for making homemade explosives. The tec......In the framework of the research project "Xsense" at the Technical University of Denmark (DTU) we are developing a simple colorimetric sensor array which can be useful in detection of explosives like DNT, TATP, HMX, RDX and identification of reagents needed for making homemade explosives....... The technology is based on an array of chemoselective compounds immobilized on a solid support. Upon exposure to the analyte in suspicion the colorimetric array changes color. Each chosen compound reacts chemo-selectively with analytes of interest. A change in a color signature indicates the presence of unknown...... explosives and volatile organic compounds (VOCs). We are working towards the selection of compounds that undergo color changes in the presence of explosives and VOCs, as well as the development of an immobilization method for the molecules. Digital imaging of the colorimetric array before and after exposure...

  8. Rapid colorimetric sensing of tetracycline antibiotics with in situ growth of gold nanoparticles. (United States)

    Shen, Li; Chen, Jing; Li, Na; He, Pingli; Li, Zhen


    A colorimetric assay utilizing the formation of gold nanoparticles was developed to detect tetracycline antibiotics in fluidic samples. Tetracycline antibiotics showed the capability of directly reducing aurate salts into atomic gold which form gold nanoparticles spontaneously under proper conditions. The resulted gold nanoparticles showed characteristic plasmon absorbance at 526 nm, which can be visualized by naked eyes or with a spectrophotometer. UV-vis absorbance of the resulted gold nanoparticles is correlated directly with the concentrations of tetracycline antibiotics in the solution, allowing for quantitative colorimetric detection of tetracycline antibiotics. Reaction conditions, such as pH, temperature, reaction time, and ionic strength were optimized. Sensitivity of the colorimetric assay can be enhanced by the addition of gold nanoparticle seeds, a LOD as low as 20 ng mL(-1) can be achieved with the help of seed particles. The colorimetric assay showed minimum interference from ethanol, methanol, urea, glucose, and other antibiotics such as sulfonamides, amino glycosides etc. Validity of the method was also evaluated on urine samples spiked with tetracycline antibiotics. The method provides a broad spectrum detection method for rapid and sensitive detection of reductive substances such as tetracycline antibiotics in liquid and biological samples.

  9. Assessment of colorimetric amplification methods in a paper-based immunoassay for diagnosis of malaria. (United States)

    Lathwal, Shefali; Sikes, Hadley D


    Colorimetric detection methods that produce results readable by eye are important for diagnostic tests in resource-limited settings. In this work, we have compared three main types of colorimetric methods - enzymatic reactions, silver deposition catalyzed by gold nanoparticles, and polymerization-based amplification - in a paper-based immunoassay for detection of Plasmodium falciparum histidine-rich protein 2, a biomarker of malarial infection. We kept the binding events in the immunoassay constant in order to isolate the effect of the detection method on the outcome of the test. We have highlighted that the optimal readout time in a test can vary significantly - ranging from immediately after addition of a visualization agent to 25 minutes after addition of a visualization agent - depending on the colorimetric method being used, and accurate time keeping is essential to prevent false positives in methods where substantial color develops over time in negative tests. We have also shown that the choice of a colorimetric method impacts the calculated limit-of-detection, the ease of visual perception of the readout, and the total cost of the assay, and therefore directly impacts the feasibility and the ease-of-use of a test in field settings.

  10. Simple colorimetric assay for dehalogenation reactivity of nanoscale zero-valent iron using 4-chlorophenol

    DEFF Research Database (Denmark)

    Hwang, Yuhoon; Mines, Paul D.; Jakobsen, Mogens Havsteen;


    Despite the wide application of nanoscale zero valent iron (nZVI) for the treatment of a plethora of pollutants through reductive reactions, reactivity evaluation of nZVI towards dehalogenation has not been standardized. In this light, it was desired to develop a simple colorimetric assay, for ve...

  11. Single Chain Fragment Variable Recombinant Antibody Functionalized Gold Nanoparticles for a Highly Sensitive Colorimetric Immunoassay (United States)

    Liu, Yang; Liu, Yi; Raymond, Raymond L.; Zeng, Xiangqun


    In this report, the peptide linker connecting scFv VH and VL domains were genetically modified to contain different amino acids (i.e. cysteine (scFv-cys) or histidines ( scFv-his)) to enable the scFv to adsorb or self-assemble onto the gold nanoparticles (NPs). The scFv-cys stabilized gold NPs were used to develop a highly sensitive colorimetric immunosensor. The scFv-cys stabilized gold NPs were characterized by UV-vis spectra, transmission electron microscope (TEM) and FT-IR. After adding the antigen rabbit IgG, the solution of scFv-cys stabilized gold NPs shows obvious visible color change from deep red to light purple due to the aggregation of the gold nanoparticles. Based on the colorimetric aggregation of scFv-cys stabilized gold NPs, the immunosensor exhibits high sensitivity with detection limit of 1.7 nM and good specificity. The good properties of the colorimetric aggregation immunosensor would be attributed to the small size of scFv and the covalent link between the scFv and gold NPs that improve the better orientation and enhance the probe density. With the advantages of speed, simplicity and specificity, the colorimetric immunoassay based on the functionalized scFv stabilized gold NPs represents a promising approach for protein analysis and clinical diagnostics. PMID:19327975

  12. Colorimetric Detection of Aniline Based on Di(hydroxymethyl)-di-(2-pyrrolyl)methane-TCNQ System

    Institute of Scientific and Technical Information of China (English)


    The blue color system of supramolecular assembly formed by di(hydroxymethyl)di-(2-pyrrolyl)methane and tetracyanoquinodimethane (TCNQ) may be used for effective and selective detection of aniline through the visual color change in CH3CN/H2O mixed solvent. The excellent properties of the system make the supramolecular assembly to be a highly selective colorimetric probe for aniline.

  13. A colorimetric and spectrophotometric method for in vitro susceptibility testing of Aspergillus species against caspofungin.

    NARCIS (Netherlands)

    Dorsthorst, D.T.A. te; Zwaaftink, R.B.; Rijs, A.J.M.M.; Meletiadis, J.; Verweij, P.E.


    The in vitro susceptibility of 45 Aspergillus fumigatus, Aspergillus flavus and Aspergillus terreus isolates against caspofungin (CAS) was assessed by the CLSI reference method with spectrophotometric reading and by a colorimetric method that employed the dye MTT. Perfect agreement was found between

  14. Feature extraction using distribution representation for colorimetric sensor arrays used as explosives detectors

    DEFF Research Database (Denmark)

    Alstrøm, Tommy Sonne; Raich, Raviv; Kostesha, Natalie;


    is required. We present a new approach of extracting features from a colorimetric sensor array based on a color distribution representation. For each sensor in the array, we construct a K-nearest neighbor classifier based on the Hellinger distances between color distribution of a test compound and the color...

  15. Data representation and feature selection for colorimetric sensor arrays used as explosives detectors

    DEFF Research Database (Denmark)

    Alstrøm, Tommy Sonne; Larsen, Jan; Kostesha, Natalie;


    Within the framework of the strategic research project Xsense at the Technical University of Denmark, we are developing a colorimetric sensor array which can be useful for detection of explosives like DNT, TNT, HMX, RDX and TATP and identification of volatile organic compounds in the presence...

  16. Iterative Magnetometer Calibration (United States)

    Sedlak, Joseph


    This paper presents an iterative method for three-axis magnetometer (TAM) calibration that makes use of three existing utilities recently incorporated into the attitude ground support system used at NASA's Goddard Space Flight Center. The method combines attitude-independent and attitude-dependent calibration algorithms with a new spinning spacecraft Kalman filter to solve for biases, scale factors, nonorthogonal corrections to the alignment, and the orthogonal sensor alignment. The method is particularly well-suited to spin-stabilized spacecraft, but may also be useful for three-axis stabilized missions given sufficient data to provide observability.

  17. Automaticity or active control

    DEFF Research Database (Denmark)

    Tudoran, Ana Alina; Olsen, Svein Ottar

    aspects of the construct, such as routine, inertia, automaticity, or very little conscious deliberation. The data consist of 2962 consumers participating in a large European survey. The results show that habit strength significantly moderates the association between satisfaction and action loyalty, and......This study addresses the quasi-moderating role of habit strength in explaining action loyalty. A model of loyalty behaviour is proposed that extends the traditional satisfaction–intention–action loyalty network. Habit strength is conceptualised as a cognitive construct to refer to the psychological......, respectively, between intended loyalty and action loyalty. At high levels of habit strength, consumers are more likely to free up cognitive resources and incline the balance from controlled to routine and automatic-like responses....

  18. Automatic Ultrasound Scanning

    DEFF Research Database (Denmark)

    Moshavegh, Ramin

    Medical ultrasound has been a widely used imaging modality in healthcare platforms for examination, diagnostic purposes, and for real-time guidance during surgery. However, despite the recent advances, medical ultrasound remains the most operator-dependent imaging modality, as it heavily relies...... on the user adjustments on the scanner interface to optimize the scan settings. This explains the huge interest in the subject of this PhD project entitled “AUTOMATIC ULTRASOUND SCANNING”. The key goals of the project have been to develop automated techniques to minimize the unnecessary settings...... on the scanners, and to improve the computer-aided diagnosis (CAD) in ultrasound by introducing new quantitative measures. Thus, four major issues concerning automation of the medical ultrasound are addressed in this PhD project. They touch upon gain adjustments in ultrasound, automatic synthetic aperture image...

  19. Automatic trend estimation

    CERN Document Server

    Vamos¸, C˘alin


    Our book introduces a method to evaluate the accuracy of trend estimation algorithms under conditions similar to those encountered in real time series processing. This method is based on Monte Carlo experiments with artificial time series numerically generated by an original algorithm. The second part of the book contains several automatic algorithms for trend estimation and time series partitioning. The source codes of the computer programs implementing these original automatic algorithms are given in the appendix and will be freely available on the web. The book contains clear statement of the conditions and the approximations under which the algorithms work, as well as the proper interpretation of their results. We illustrate the functioning of the analyzed algorithms by processing time series from astrophysics, finance, biophysics, and paleoclimatology. The numerical experiment method extensively used in our book is already in common use in computational and statistical physics.

  20. Automatic food decisions

    DEFF Research Database (Denmark)

    Mueller Loose, Simone

    Consumers' food decisions are to a large extent shaped by automatic processes, which are either internally directed through learned habits and routines or externally influenced by context factors and visual information triggers. Innovative research methods such as eye tracking, choice experiments...... and food diaries allow us to better understand the impact of unconscious processes on consumers' food choices. Simone Mueller Loose will provide an overview of recent research insights into the effects of habit and context on consumers' food choices....

  1. Drift-insensitive distributed calibration of probe microscope scanner in nanometer range: Virtual mode (United States)

    Lapshin, Rostislav V.


    A method of distributed calibration of a probe microscope scanner is suggested. The main idea consists in a search for a net of local calibration coefficients (LCCs) in the process of automatic measurement of a standard surface, whereby each point of the movement space of the scanner can be characterized by a unique set of scale factors. Feature-oriented scanning (FOS) methodology is used as a basis for implementation of the distributed calibration permitting to exclude in situ the negative influence of thermal drift, creep and hysteresis on the obtained results. Possessing the calibration database enables correcting in one procedure all the spatial systematic distortions caused by nonlinearity, nonorthogonality and spurious crosstalk couplings of the microscope scanner piezomanipulators. To provide high precision of spatial measurements in nanometer range, the calibration is carried out using natural standards - constants of crystal lattice. One of the useful modes of the developed calibration method is a virtual mode. In the virtual mode, instead of measurement of a real surface of the standard, the calibration program makes a surface image "measurement" of the standard, which was obtained earlier using conventional raster scanning. The application of the virtual mode permits simulation of the calibration process and detail analysis of raster distortions occurring in both conventional and counter surface scanning. Moreover, the mode allows to estimate the thermal drift and the creep velocities acting while surface scanning. Virtual calibration makes possible automatic characterization of a surface by the method of scanning probe microscopy (SPM).

  2. Automatization of lexicographic work

    Directory of Open Access Journals (Sweden)

    Iztok Kosem


    Full Text Available A new approach to lexicographic work, in which the lexicographer is seen more as a validator of the choices made by computer, was recently envisaged by Rundell and Kilgarriff (2011. In this paper, we describe an experiment using such an approach during the creation of Slovene Lexical Database (Gantar, Krek, 2011. The corpus data, i.e. grammatical relations, collocations, examples, and grammatical labels, were automatically extracted from 1,18-billion-word Gigafida corpus of Slovene. The evaluation of the extracted data consisted of making a comparison between the time spent writing a manual entry and a (semi-automatic entry, and identifying potential improvements in the extraction algorithm and in the presentation of data. An important finding was that the automatic approach was far more effective than the manual approach, without any significant loss of information. Based on our experience, we would propose a slightly revised version of the approach envisaged by Rundell and Kilgarriff in which the validation of data is left to lower-level linguists or crowd-sourcing, whereas high-level tasks such as meaning description remain the domain of lexicographers. Such an approach indeed reduces the scope of lexicographer’s work, however it also results in the ability of bringing the content to the users more quickly.

  3. Nanoparticle-catalyzed reductive bleaching for fabricating turn-off and enzyme-free amplified colorimetric bioassays. (United States)

    Li, Wei; Qiang, Weibing; Li, Jie; Li, Hui; Dong, Yifan; Zhao, Yaju; Xu, Danke


    Nanoparticle-catalyzed reductive bleaching reactions of colored substrates are emerging as a class of novel indicator reactions for fabricating enzyme-free amplified colorimetric biosensing (turn-off mode), which are exactly opposite to the commonly used oxidative coloring processes of colorless substrates in traditional enzyme-catalyzed amplified colorimetric bioassays (turn-on mode). In this work, a simple theoretical analysis shows that the sensitivity of this colorimetric bioassay can be improved by increasing the amplification factor (kcatΔt), or enhancing the binding affinity between analyte and receptor (Kd), or selecting the colored substrates with high extinction coefficients (ε). Based on this novel strategy, we have developed a turn-off and cost-effective amplified colorimetric thrombin aptasensor. This aptasensor made full use of sandwich binding of two affinity aptamers for increased specificity, magnetic particles for easy separation and enrichment, and gold nanoparticle (AuNP)-catalyzed reductive bleaching reaction to generate the amplified colorimetric signal. With 4-nitrophenol (4-NP) as the non-dye colored substrate, colorimetric bioassay of thrombin was achieved by the endpoint method with a detection limit of 91pM. In particular, when using methylene blue (MB) as the substrate, for the first time, a more convenient and efficient kinetic-based colorimetric thrombin bioassay was achieved without the steps of acidification termination and magnetic removal of particles, with a low detection limit of 10pM, which was superior to the majority of the existing colorimetric thrombin aptasensors. The proposed colorimetric protocol is expected to hold great promise in field analysis and point-of-care applications.

  4. Smart Calibration of Excavators

    DEFF Research Database (Denmark)

    Bro, Marie; Døring, Kasper; Ellekilde, Lars-Peter


    Excavators dig holes. But where is the bucket? The purpose of this report is to treat four different problems concerning calibrations of position indicators for excavators in operation at concrete construction sites. All four problems are related to the question of how to determine the precise ge...

  5. Calibrating Communication Competencies (United States)

    Surges Tatum, Donna


    The Many-faceted Rasch measurement model is used in the creation of a diagnostic instrument by which communication competencies can be calibrated, the severity of observers/raters can be determined, the ability of speakers measured, and comparisons made between various groups.

  6. Entropic calibration revisited

    Energy Technology Data Exchange (ETDEWEB)

    Brody, Dorje C. [Blackett Laboratory, Imperial College, London SW7 2BZ (United Kingdom)]. E-mail:; Buckley, Ian R.C. [Centre for Quantitative Finance, Imperial College, London SW7 2AZ (United Kingdom); Constantinou, Irene C. [Blackett Laboratory, Imperial College, London SW7 2BZ (United Kingdom); Meister, Bernhard K. [Blackett Laboratory, Imperial College, London SW7 2BZ (United Kingdom)


    The entropic calibration of the risk-neutral density function is effective in recovering the strike dependence of options, but encounters difficulties in determining the relevant greeks. By use of put-call reversal we apply the entropic method to the time reversed economy, which allows us to obtain the spot price dependence of options and the relevant greeks.

  7. Calibration with Absolute Shrinkage

    DEFF Research Database (Denmark)

    Øjelund, Henrik; Madsen, Henrik; Thyregod, Poul


    is suggested to cope with the singular design matrix most often seen in chemometric calibration. Furthermore, the proposed algorithm may be generalized to all convex norms like Sigma/beta (j)/(gamma) where gamma greater than or equal to 1, i.e. a method that continuously varies from ridge regression...

  8. LOFAR facet calibration

    CERN Document Server

    van Weeren, R J; Hardcastle, M J; Shimwell, T W; Rafferty, D A; Sabater, J; Heald, G; Sridhar, S S; Dijkema, T J; Brunetti, G; Brüggen, M; Andrade-Santos, F; Ogrean, G A; Röttgering, H J A; Dawson, W A; Forman, W R; de Gasperin, F; Jones, C; Miley, G K; Rudnick, L; Sarazin, C L; Bonafede, A; Best, P N; Bîrzan, L; Cassano, R; Chyży, K T; Croston, J H; Ensslin, T; Ferrari, C; Hoeft, M; Horellou, C; Jarvis, M J; Kraft, R P; Mevius, M; Intema, H T; Murray, S S; Orrú, E; Pizzo, R; Simionescu, A; Stroe, A; van der Tol, S; White, G J


    LOFAR, the Low-Frequency Array, is a powerful new radio telescope operating between 10 and 240 MHz. LOFAR allows detailed sensitive high-resolution studies of the low-frequency radio sky. At the same time LOFAR also provides excellent short baseline coverage to map diffuse extended emission. However, producing high-quality deep images is challenging due to the presence of direction dependent calibration errors, caused by imperfect knowledge of the station beam shapes and the ionosphere. Furthermore, the large data volume and presence of station clock errors present additional difficulties. In this paper we present a new calibration scheme, which we name facet calibration, to obtain deep high-resolution LOFAR High Band Antenna images using the Dutch part of the array. This scheme solves and corrects the direction dependent errors in a number of facets that cover the observed field of view. Facet calibration provides close to thermal noise limited images for a typical 8 hr observing run at $\\sim$ 5arcsec resolu...

  9. Measurement System & Calibration report

    DEFF Research Database (Denmark)

    Kock, Carsten Weber; Vesth, Allan

    This Measurement System & Calibration report is describing DTU’s measurement system installed at a specific wind turbine. A major part of the sensors has been installed by others (see [1]) the rest of the sensors have been installed by DTU. The results of the measurements, described in this report...

  10. NVLAP calibration laboratory program

    Energy Technology Data Exchange (ETDEWEB)

    Cigler, J.L.


    This paper presents an overview of the progress up to April 1993 in the development of the Calibration Laboratories Accreditation Program within the framework of the National Voluntary Laboratory Accreditation Program (NVLAP) at the National Institute of Standards and Technology (NIST).

  11. Liquid Metering Centrifuge Sticks (LMCS): A Centrifugal Approach to Metering Known Sample Volumes for Colorimetric Solid Phase Extraction (C-SPE) (United States)

    Gazda, Daniel B.; Schultz, John R.; Clarke, Mark S.


    Phase separation is one of the most significant obstacles encountered during the development of analytical methods for water quality monitoring in spacecraft environments. Removing air bubbles from water samples prior to analysis is a routine task on earth; however, in the absence of gravity, this routine task becomes extremely difficult. This paper details the development and initial ground testing of liquid metering centrifuge sticks (LMCS), devices designed to collect and meter a known volume of bubble-free water in microgravity. The LMCS uses centrifugal force to eliminate entrapped air and reproducibly meter liquid sample volumes for analysis with Colorimetric Solid Phase Extraction (C-SPE). C-SPE is a sorption-spectrophotometric platform that is being developed as a potential spacecraft water quality monitoring system. C-SPE utilizes solid phase extraction membranes impregnated with analyte-specific colorimetric reagents to concentrate and complex target analytes in spacecraft water samples. The mass of analyte extracted from the water sample is determined using diffuse reflectance (DR) data collected from the membrane surface and an analyte-specific calibration curve. The analyte concentration can then be calculated from the mass of extracted analyte and the volume of the sample analyzed. Previous flight experiments conducted in microgravity conditions aboard the NASA KC-135 aircraft demonstrated that the inability to collect and meter a known volume of water using a syringe was a limiting factor in the accuracy of C-SPE measurements. Herein, results obtained from ground based C-SPE experiments using ionic silver as a test analyte and either the LMCS or syringes for sample metering are compared to evaluate the performance of the LMCS. These results indicate very good agreement between the two sample metering methods and clearly illustrate the potential of utilizing centrifugal forces to achieve phase separation and metering of water samples in microgravity.

  12. A Colorimetric Method for the Determination of the Exhaustion Level of Granular Activated Carbons Used in Rum Production

    Directory of Open Access Journals (Sweden)

    Harold Crespo Sariol


    Full Text Available Spectrophotometric measurement applied on saturated granular activated carbon (GAC is not yet explored. A colorimetric method in the visible range has been developed in order to determine the exhaustion level of GAC used in rum production. Aqueous ammonia solution has been used as an indicative agent to determine the extraction rate of taste compounds within the rum production process and the exhaustion degree of the GAC. The colorimetric results showed excellent correlation with the iodine number and the contact pH. The proposed colorimetric method opens possibilities for rum producers to improve the management and economical use of the activated carbon at the industrial scale.

  13. Mercury CEM Calibration

    Energy Technology Data Exchange (ETDEWEB)

    John F. Schabron; Joseph F. Rovani; Susan S. Sorini


    The Clean Air Mercury Rule (CAMR) which was published in the Federal Register on May 18, 2005, requires that calibration of mercury continuous emissions monitors (CEMs) be performed with NIST-traceable standards. Western Research Institute (WRI) is working closely with the Electric Power Research Institute (EPRI), the National Institute of Standards and Technology (NIST), and the Environmental Protection Agency (EPA) to facilitate the development of the experimental criteria for a NIST traceability protocol for dynamic elemental mercury vapor generators. The traceability protocol will be written by EPA. Traceability will be based on the actual analysis of the output of each calibration unit at several concentration levels ranging from about 2-40 ug/m{sup 3}, and this analysis will be directly traceable to analyses by NIST using isotope dilution inductively coupled plasma/mass spectrometry (ID ICP/MS) through a chain of analyses linking the calibration unit in the power plant to the NIST ID ICP/MS. Prior to this project, NIST did not provide a recommended mercury vapor pressure equation or list mercury vapor pressure in its vapor pressure database. The NIST Physical and Chemical Properties Division in Boulder, Colorado was subcontracted under this project to study the issue in detail and to recommend a mercury vapor pressure equation that the vendors of mercury vapor pressure calibration units can use to calculate the elemental mercury vapor concentration in an equilibrium chamber at a particular temperature. As part of this study, a preliminary evaluation of calibration units from five vendors was made. The work was performed by NIST in Gaithersburg, MD and Joe Rovani from WRI who traveled to NIST as a Visiting Scientist.

  14. Field calibration of cup anemometers

    DEFF Research Database (Denmark)

    Schmidt Paulsen, Uwe; Mortensen, Niels Gylling; Hansen, Jens Carsten


    A field calibration method and results are described along with the experience gained with the method. The cup anemometers to be calibrated are mounted in a row on a 10-m high rig and calibrated in the free wind against a reference cup anemometer. The method has been reported [1] to improve...... the statistical bias on the data relative to calibrations carried out in a wind tunnel. The methodology is sufficiently accurate for calibration of cup anemometers used for wind resource assessments and provides a simple, reliable and cost-effective solution to cup anemometer calibration, especially suited...

  15. Quantitative Analysis of Carbon Steel with Multi-Line Internal Standard Calibration Method Using Laser-Induced Breakdown Spectroscopy. (United States)

    Pan, Congyuan; Du, Xuewei; An, Ning; Zeng, Qiang; Wang, Shengbo; Wang, Qiuping


    A multi-line internal standard calibration method is proposed for the quantitative analysis of carbon steel using laser-induced breakdown spectroscopy (LIBS). A procedure based on the method was adopted to select the best calibration curves and the corresponding emission lines pairs automatically. Laser-induced breakdown spectroscopy experiments with carbon steel samples were performed, and C, Cr, and Mn were analyzed via the proposed method. Calibration curves of these elements were constructed via a traditional single line internal standard calibration method and a multi-line internal standard calibration method. The calibration curves obtained were evaluated with the determination coefficient, the root mean square error of cross-validation, and the average relative error of cross-validation. All of the parameters were improved significantly with the proposed method. The results show that accurate and stable calibration curves can be obtained efficiently via the multi-line internal standard calibration method.

  16. Mercury Calibration System

    Energy Technology Data Exchange (ETDEWEB)

    John Schabron; Eric Kalberer; Joseph Rovani; Mark Sanderson; Ryan Boysen; William Schuster


    U.S. Environmental Protection Agency (EPA) Performance Specification 12 in the Clean Air Mercury Rule (CAMR) states that a mercury CEM must be calibrated with National Institute for Standards and Technology (NIST)-traceable standards. In early 2009, a NIST traceable standard for elemental mercury CEM calibration still does not exist. Despite the vacature of CAMR by a Federal appeals court in early 2008, a NIST traceable standard is still needed for whatever regulation is implemented in the future. Thermo Fisher is a major vendor providing complete integrated mercury continuous emissions monitoring (CEM) systems to the industry. WRI is participating with EPA, EPRI, NIST, and Thermo Fisher towards the development of the criteria that will be used in the traceability protocols to be issued by EPA. An initial draft of an elemental mercury calibration traceability protocol was distributed for comment to the participating research groups and vendors on a limited basis in early May 2007. In August 2007, EPA issued an interim traceability protocol for elemental mercury calibrators. Various working drafts of the new interim traceability protocols were distributed in late 2008 and early 2009 to participants in the Mercury Standards Working Committee project. The protocols include sections on qualification and certification. The qualification section describes in general terms tests that must be conducted by the calibrator vendors to demonstrate that their calibration equipment meets the minimum requirements to be established by EPA for use in CAMR monitoring. Variables to be examined include linearity, ambient temperature, back pressure, ambient pressure, line voltage, and effects of shipping. None of the procedures were described in detail in the draft interim documents; however they describe what EPA would like to eventually develop. WRI is providing the data and results to EPA for use in developing revised experimental procedures and realistic acceptance criteria based on

  17. Colorimetric grading scale can promote the standardization of experiential and sensory evaluation in quality control of traditional Chinese medicines.

    Directory of Open Access Journals (Sweden)

    Jia-bo Wang

    Full Text Available Experiential and sensory evaluation is an ancient method that remains important in the current quality control system of Traditional Chinese Medicines (TCMs. The process is rapid and convenient when evaluating the quality of crude materials in TCM markets. However, sensory evaluation has been met with skepticism because it is mainly based on experience and lacks a scientific basis. In this study, rhubarb was selected to demonstrate how color-based sensory evaluation could differentiate the quality of herbal medicines objectively. The colors of the rhubarb samples, expressed as RGB values, were obtained from different parts and forms of the plant, including the plant's surface, fracture surface color, and a powdered form with or without treatment with a color-developing reagent. We first divided the rhubarb samples into three grades based on the total content of five hydroxyanthraquinone derivatives, the major pharmacological components in rhubarb. Then, a three-layer back-propagation artificial neural network (BP-ANN, calibrated with selected training samples, was used to correlate the quality of the rhubarb with its color. The color of the rhubarb powder after coloration attained the highest accuracy (92.3% in predicting the quality grade of the test samples with the established artificial neural networks. Finally, a standardized colorimetric grading scale was created based on the spatial distribution of the rhubarb samples in a two-dimensional chromaticity diagram according to the colors of the powdered rhubarb after color enhancement. By comparing the color between the scale and the tested samples, similar to performing a pH test with indicator paper, subjects without sensory evaluation experience could quickly determine the quality grade of rhubarb. This work illustrates the technical feasibility of the color-based grading of rhubarb quality and offers references for quantifying and standardizing the sensory evaluation of TCMs, foods and

  18. Landsat 8 on-orbit characterization and calibration system (United States)

    Micijevic, Esad; Morfitt, Ron; Choate, Michael J.


    The Landsat Data Continuity Mission (LDCM) is planning to launch the Landsat 8 satellite in December 2012, which continues an uninterrupted record of consistently calibrated globally acquired multispectral images of the Earth started in 1972. The satellite will carry two imaging sensors: the Operational Land Imager (OLI) and the Thermal Infrared Sensor (TIRS). The OLI will provide visible, near-infrared and short-wave infrared data in nine spectral bands while the TIRS will acquire thermal infrared data in two bands. Both sensors have a pushbroom design and consequently, each has a large number of detectors to be characterized. Image and calibration data downlinked from the satellite will be processed by the U.S. Geological Survey (USGS) Earth Resources Observation and Science (EROS) Center using the Landsat 8 Image Assessment System (IAS), a component of the Ground System. In addition to extracting statistics from all Earth images acquired, the IAS will process and trend results from analysis of special calibration acquisitions, such as solar diffuser, lunar, shutter, night, lamp and blackbody data, and preselected calibration sites. The trended data will be systematically processed and analyzed, and calibration and characterization parameters will be updated using both automatic and customized manual tools. This paper describes the analysis tools and the system developed to monitor and characterize on-orbit performance and calibrate the Landsat 8 sensors and image data products.

  19. Automatic Configuration in NTP

    Institute of Scientific and Technical Information of China (English)

    Jiang Zongli(蒋宗礼); Xu Binbin


    NTP is nowadays the most widely used distributed network time protocol, which aims at synchronizing the clocks of computers in a network and keeping the accuracy and validation of the time information which is transmitted in the network. Without automatic configuration mechanism, the stability and flexibility of the synchronization network built upon NTP protocol are not satisfying. P2P's resource discovery mechanism is used to look for time sources in a synchronization network, and according to the network environment and node's quality, the synchronization network is constructed dynamically.

  20. Self Calibrating Flow Estimation in Waste Water Pumping Stations

    DEFF Research Database (Denmark)

    Kallesøe, Carsten Skovmose; Knudsen, Torben


    Knowledge about where waste water is flowing in waste water networks is essential to optimize the operation of the network pumping stations. However, installation of flow sensors is expensive and requires regular maintenance. This paper proposes an alternative approach where the pumps and the waste...... water pit are used for estimating both the inflow and the pump flow of the pumping station. Due to the nature of waste water, the waste water pumps are heavily affected by wear and tear. To compensate for the wear of the pumps, the pump parameters, used for the flow estimation, are automatically...... calibrated. This calibration is done based on data batches stored at each pump cycle, hence makes the approach a self calibrating system. The approach is tested on a pumping station operating in a real waste water network....

  1. The New Approach to Camera Calibration - GCPs or TLS Data? (United States)

    Markiewicz, J.; Podlasiak, P.; Kowalczyk, M.; Zawieska, D.


    Camera calibration is one of the basic photogrammetric tasks responsible for the quality of processed products. The majority of calibration is performed with a specially designed test field or during the self-calibration process. The research presented in this paper aims to answer the question of whether it is necessary to use control points designed in the standard way for determination of camera interior orientation parameters. Data from close-range laser scanning can be used as an alternative. The experiments shown in this work demonstrate the potential of laser measurements, since the number of points that may be involved in the calculation is much larger than that of commonly used ground control points. The problem which still exists is the correct and automatic identification of object details in the image, taken with a tested camera, as well as in the data set registered with the laser scanner.

  2. The Calibration Reference Data System (United States)

    Greenfield, P.; Miller, T.


    We describe a software architecture and implementation for using rules to determine which calibration files are appropriate for calibrating a given observation. This new system, the Calibration Reference Data System (CRDS), replaces what had been previously used for the Hubble Space Telescope (HST) calibration pipelines, the Calibration Database System (CDBS). CRDS will be used for the James Webb Space Telescope (JWST) calibration pipelines, and is currently being used for HST calibration pipelines. CRDS can be easily generalized for use in similar applications that need a rules-based system for selecting the appropriate item for a given dataset; we give some examples of such generalizations that will likely be used for JWST. The core functionality of the Calibration Reference Data System is available under an Open Source license. CRDS is briefly contrasted with a sampling of other similar systems used at other observatories.

  3. Ultrafast colorimetric determination of predominant protein structure evolution with gold nanoplasmonic particles (United States)

    Kim, Hye Young; Choi, Inhee


    The intracellular and extracellular accumulation of disordered proteins and aggregated proteins occurs in many protein conformational diseases, such as aging-related neurodegeneration and alcoholic liver diseases. However, the conventional methods to study protein structural changes are limited for the rapid detection and monitoring of protein aggregation because of long incubation times (i.e., usually several days), complicated sample pretreatment steps, and expensive instrumentation. Here, we describe an ultrafast colorimetric method for the real-time monitoring of protein structure evolution and the determination of predominant structures via nanoparticle-assisted protein aggregation. During the aggregation process, nanoparticles act as nucleation cores, which form networks depending on the structures of the protein aggregates, and accelerate the kinetics of the protein aggregation. Simultaneously, these nanoparticles exhibit colorimetric responses according to their embedded shapes (e.g., fibrillar and amorphous) on the protein aggregates. We observed distinct spectral shifts and concomitant colorimetric responses of concentration- and type-dependent protein aggregation with the naked eye within a few minutes (pH levels, high temperature, and chemicals. These findings suggest that the proposed method is an easy way to study the molecular biophysics of protein aggregation and to rapidly screen anti-aggregation drugs for protein conformational diseases.The intracellular and extracellular accumulation of disordered proteins and aggregated proteins occurs in many protein conformational diseases, such as aging-related neurodegeneration and alcoholic liver diseases. However, the conventional methods to study protein structural changes are limited for the rapid detection and monitoring of protein aggregation because of long incubation times (i.e., usually several days), complicated sample pretreatment steps, and expensive instrumentation. Here, we describe an

  4. Lidar calibration experiments

    DEFF Research Database (Denmark)

    Ejsing Jørgensen, Hans; Mikkelsen, T.; Streicher, J.


    A series of atmospheric aerosol diffusion experiments combined with lidar detection was conducted to evaluate and calibrate an existing retrieval algorithm for aerosol backscatter lidar systems. The calibration experiments made use of two (almost) identical mini-lidar systems for aerosol cloud...... detection to test the reproducibility and uncertainty of lidars. Lidar data were obtained from both single-ended and double-ended Lidar configurations. A backstop was introduced in one of the experiments and a new method was developed where information obtained from the backstop can be used in the inversion...... algorithm. Independent in-situ aerosol plume concentrations were obtained from a simultaneous tracer gas experiment with SF6, and comparisons with the two lidars were made. The study shows that the reproducibility of the lidars is within 15%, including measurements from both sides of a plume...

  5. Optical tweezers absolute calibration

    CERN Document Server

    Dutra, R S; Neto, P A Maia; Nussenzveig, H M


    Optical tweezers are highly versatile laser traps for neutral microparticles, with fundamental applications in physics and in single molecule cell biology. Force measurements are performed by converting the stiffness response to displacement of trapped transparent microspheres, employed as force transducers. Usually, calibration is indirect, by comparison with fluid drag forces. This can lead to discrepancies by sizable factors. Progress achieved in a program aiming at absolute calibration, conducted over the past fifteen years, is briefly reviewed. Here we overcome its last major obstacle, a theoretical overestimation of the peak stiffness, within the most employed range for applications, and we perform experimental validation. The discrepancy is traced to the effect of primary aberrations of the optical system, which are now included in the theory. All required experimental parameters are readily accessible. Astigmatism, the dominant effect, is measured by analyzing reflected images of the focused laser spo...

  6. Astrid-2 SSC ASUMagnetic Calibration

    DEFF Research Database (Denmark)

    Primdahl, Fritz


    Report of the inter calibration between the starcamera and the fluxgate magnetometer onboard the ASTRID-2 satellite. This calibration was performed in the night between the 15. and 16. May 1997 at the Lovö magnetic observatory.......Report of the inter calibration between the starcamera and the fluxgate magnetometer onboard the ASTRID-2 satellite. This calibration was performed in the night between the 15. and 16. May 1997 at the Lovö magnetic observatory....

  7. Calibration Facilities for NIF

    Energy Technology Data Exchange (ETDEWEB)

    Perry, T.S.


    The calibration facilities will be dynamic and will change to meet the needs of experiments. Small sources, such as the Manson Source should be available to everyone at any time. Carrying out experiments at Omega is providing ample opportunity for practice in pre-shot preparation. Hopefully, the needs that are demonstrated in these experiments will assure the development of (or keep in service) facilities at each of the laboratories that will be essential for in-house preparation for experiments at NIF.

  8. Mesoscale hybrid calibration artifact (United States)

    Tran, Hy D.; Claudet, Andre A.; Oliver, Andrew D.


    A mesoscale calibration artifact, also called a hybrid artifact, suitable for hybrid dimensional measurement and the method for make the artifact. The hybrid artifact has structural characteristics that make it suitable for dimensional measurement in both vision-based systems and touch-probe-based systems. The hybrid artifact employs the intersection of bulk-micromachined planes to fabricate edges that are sharp to the nanometer level and intersecting planes with crystal-lattice-defined angles.

  9. Calibration of Underwater Sound Transducers

    Directory of Open Access Journals (Sweden)

    H.R.S. Sastry


    Full Text Available The techniques of calibration of underwater sound transducers for farfield, near-field and closed environment conditions are reviewed in this paper .The design of acoustic calibration tank is mentioned. The facilities available at Naval Physical & Oceanographic Laboratory, Cochin for calibration of transducers are also listed.

  10. Internet-based calibration of a multifunction calibrator

    Energy Technology Data Exchange (ETDEWEB)



    A new way of providing calibration services is evolving which employs the Internet to expand present capabilities and make the calibration process more interactive. Sandia National Laboratories and the National Institute of Standards and Technology are collaborating to set up and demonstrate a remote calibration of multifunction calibrators using this Internet-based technique that is becoming known as e-calibration. This paper describes the measurement philosophy and the Internet resources that can provide real-time audio/video/data exchange, consultation and training, as well as web-accessible test procedures, software and calibration reports. The communication system utilizes commercial hardware and software that should be easy to integrate into most calibration laboratories.

  11. Comparison of automatic control systems (United States)

    Oppelt, W


    This report deals with a reciprocal comparison of an automatic pressure control, an automatic rpm control, an automatic temperature control, and an automatic directional control. It shows the difference between the "faultproof" regulator and the actual regulator which is subject to faults, and develops this difference as far as possible in a parallel manner with regard to the control systems under consideration. Such as analysis affords, particularly in its extension to the faults of the actual regulator, a deep insight into the mechanism of the regulator process.

  12. Quantitative colorimetric-imaging analysis of nickel in iron meteorites. (United States)

    Zamora, L Lahuerta; López, P Alemán; Fos, G M Antón; Algarra, R Martín; Romero, A M Mellado; Calatayud, J Martínez


    A quantitative analytical imaging approach for determining the nickel content of metallic meteorites is proposed. The approach uses a digital image of a series of standard solutions of the nickel-dimethylglyoxime coloured chelate and a meteorite sample solution subjected to the same treatment as the nickel standards for quantitation. The image is processed with suitable software to assign a colour-dependent numerical value (analytical signal) to each standard. Such a value is directly proportional to the analyte concentration, which facilitates construction of a calibration graph where the value for the unknown sample can be interpolated to calculate the nickel content of the meteorite. The results thus obtained were validated by comparison with the official, ISO-endorsed spectrophotometric method for nickel. The proposed method is fairly simple and inexpensive; in fact, it uses a commercially available digital camera as measuring instrument and the images it provides are processed with highly user-friendly public domain software (specifically, ImageJ, developed by the National Institutes of Health and freely available for download on the Internet). In a scenario dominated by increasingly sophisticated and expensive equipment, the proposed method provides a cost-effective alternative based on simple, robust hardware that is affordable and can be readily accessed worldwide. This can be especially advantageous for countries were available resources for analytical equipment investments are scant. The proposed method is essentially an adaptation of classical chemical analysis to current, straightforward, robust, cost-effective instrumentation.

  13. Characteristics of a new automatic hail recorder (United States)

    Löffler-Mang, Martin; Schön, Dominik; Landry, Markus


    An automatic hail sensor was developed, based on signal production with microphones, a quick signal analysis and recording possibility. For this hail recorder (HARE) small piezo-electric microphones inside a Makrolon body are used to detect hailstones. The prototype has an octagonal shape, two microphones on the top and bottom plates situated in the middle of the device, and an electronic board. A hailstone striking the surface produces waves on the sensor body and a voltage in the piezo-electric microphones. Each hail event is stored in the internal memory including the time and date. The memory can be read out via a USB port at any time after one or more hail events. HARE was tested and calibrated with the help of a newly constructed pneumatic hail gun. The voltage signal at the top plate microphone of HARE increases linearly proportional to hailstone momentum, whereas at the bottom plate it increases linearly proportional to hailstone kinetic energy. For large hailstones the accuracy of HARE is in the order of 10%. Calibration of HARE is still in progress and it has not been tested in real hailfalls as yet. An online device as well as an autonomous one is available for a large number of possible applications. Lately there has been interest to use HARE at solar power plants in Southern Europe to prevent the expensive modules from becoming damaged. Perhaps HARE could also participate in new and existing hail observing networks.

  14. Colorimetric chemosensor for multi-signaling detection of metal ions using pyrrole based Schiff bases. (United States)

    Udhayakumari, Duraisamy; Velmathi, Sivan


    Pyrrole based Schiff bases act as a highly sensitive probe for metal ions in aqueous medium. Both receptors R1 and R2 are sensitive towards Fe(3+), Cu(2+), Hg(2+) and Cr(3+) among the other metal ions. The sensing ability of the receptors are investigated via colorimetric, optical and emission spectroscopic studies. The binding stoichiometries of R1 and R2 with metal ions have been determined as 2:1 by using Job's plot. The colorimetric receptors exhibited high sensitivity with a low detection limit of μM levels. In the presence of metal ions both receptors shows fluorescence quenching. This might be due to the photo induced electron transfer mechanism. The quenching constant was further determined using Stern-Volmer plot.

  15. Enzyme-free colorimetric detection systems based on the DNA strand displacement competition reaction (United States)

    Zhang, Z.; Birkedal, V.; Gothelf, K. V.


    The strand displacement competition assay is based on the dynamic equilibrium of the competitive hybridization of two oligonucleotides (A and B) to a third oligonucleotide (S). In the presence of an analyte that binds to a specific affinity-moiety conjugated to strand B, the equilibrium shifts, which can be detected by a shift in the fluorescence resonance energy transfer signal between dyes attached to the DNA strands. In the present study we have integrated an ATP aptamer in the strand B and demonstrated the optical detection of ATP. Furthermore we explore a new readout method using a split G-quadruplex DNAzyme for colorimetric readout of the detection of streptavidin by the naked eye. Finally, we integrate the whole G-quadruplex DNAzyme system in a single DNA strand and show that it is applicable to colorimetric detection.

  16. Colorimetric and fluorometric detection of neomycin based on conjugated polydiacetylene supramolecules. (United States)

    Zhou, Guodong; Wang, Fang; Wang, Huilin; Kambam, Srinivasulu; Chen, Xiaoqiang


    Utilizing the colorimetric and fluorogenic changes, a system based on polydiacetylenes (PDAs) is developed for the detection of neomycin. The PDA supramolecules polymerized from the mixed liposome composed of N-(3-hydroxyphenyl)pentacosa-10,12-diynamide (PCDA-AP) and pentacosa-10,12-diynoic acid (PCDA) at an optimized ratio of 1:9 display a unique colorimetric change (blue to red) and fluorescent enhancement in the presence of neomycin. The detection limit for neomycin is estimated to be 2.55 × 10(-7) M by the fluorogenic method. The optical changes induced by neomycin can be attributed to the disruption of the hydrogen bonding between phenol and carboxylic acid from PCDA-AP and PCDA.

  17. A Rapid Colorimetric Method Reveals Fraudulent Substitutions in Sea Urchin Roe Marketed in Sardinia (Italy). (United States)

    Meloni, Domenico; Spina, Antonio; Satta, Gianluca; Chessa, Vittorio


    In recent years, besides the consumption of fresh sea urchin specimens, the demand of minimally-processed roe has grown considerably. This product has made frequent consumption in restaurants possible and frauds are becoming widespread with the partial replacement of sea urchin roe with surrogates that are similar in colour. One of the main factors that determines the quality of the roe is its colour and small differences in colour scale cannot be easily discerned by the consumers. In this study we have applied a rapid colorimetric method for reveal the fraudulent partial substitution of semi-solid sea urchin roe with liquid egg yolk. Objective assessment of whiteness (L*), redness (a*), yellowness (b*), hue (h*), and chroma (C*) was carried out with a digital spectrophotometer using the CIE L*a*b* colour measurement system. The colorimetric method highlighted statistically significant differences among sea urchin roe and liquid egg yolk that could be easily discerned quantitatively.

  18. Comparative analysis of colorimetric staining in skin using open-source software (United States)

    Billings, Paul C; Sanzari, Jenine K.; Kennedy, Ann R.; Cengel, Keith A.; Seykora, John T.


    Colorimetric staining techniques such as immunohistochemistry (IHC), immunofluorescence (IF) and histochemistry (HC) provide useful information regarding the localization and relative amount of a molecule/substance in skin. We have developed a novel, straightforward method to assess colorimetric staining by combining features from two open-source software programs. As a proof of principle, we demonstrate the utility of this approach by analyzing changes in skin melanin deposition during the radiation-induced tanning response of Yucatan mini-pigs. This method includes a visualization step to validate the accuracy of color selection before quantitation to ensure accuracy. The data show that this method is robust and will provide a means to obtain accurate comparative analyses of staining in IHC/IF/HC samples. PMID:25393687

  19. Functionalized magnetic microparticle-based colorimetric platform for influenza A virus detection (United States)

    Chen, Chaohui; Zou, Zhong; Chen, Lu; Ji, Xinghu; He, Zhike


    A colorimetric platform for influenza A virus detection was developed by using the high efficiency of enzymatic catalysis and the reduction of gold ions with hydrogen peroxide. Aptamer-functionalized magnetic microparticles were synthesized to capture the influenza A virus. This was followed by the binding of ConA-GOx-AuNPs to the H3N2 virus through the ConA-glycan interaction. The sandwich complex was subsequently dispersed in glucose solution to trigger an enzymatic reaction to produce hydrogen peroxide, which controlled the growth of gold nanoparticles and produced colored solutions. The determination of H3N2 concentration was realized by comparing the two differently colored gold nanoparticles. This method could detect the target virus as low as 11.16 μg ml-1. Furthermore, it opens new opportunities for sensitive and colorimetric detection of viruses and proteins.

  20. Colorimetric evaluation of iPhone apps for colour vision tests based on the Ishihara test. (United States)

    Dain, Stephen J; AlMerdef, Ali


    Given the versatility of smart phone displays, it was inevitable that applications (apps) providing colour vision testing would appear as an option. In this study, the colorimetric characteristics of five available iPhone apps for colour vision testing are assessed as a prequel to possible clinical evaluation. The colours of the displays produced by the apps are assessed with reference to the colours of a printed Ishihara test. The visual task is assessed on the basis of the colour differences and the alignment to the dichromatic confusion lines. The apps vary in quality and while some are colorimetrically acceptable, there are also some problems with their construction in making them a clinically useful app rather than curiosity driven self-testing. There is no reason why, in principle, a suitable test cannot be designed for smart phones.

  1. A Highly Selective Colorimetric Sensor for Cysteine in Water Solution and Bovine Serum Albumin

    Directory of Open Access Journals (Sweden)

    Xuefang Shang


    Full Text Available A simple colorimetric sensor, 2-bromonaphthalene-1,4-dione, has been developed for the Cysteine detection. The sensor showed its best performance in a mixture of ethanol and HEPES (5 : 5, v/v solution at pH of 7.0. The results of UV-vis and fluorescence indicated that 2-bromonaphthalene-1,4-dione was selective and sensitive for Cysteine detection without the interference of other amino acids (Cysteine, Alanine, Arginine, Aspartinie, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Proline, Serine, Threonine, Phenylalanine, Valine, Tryptophan, and Hydroxyproline. 2-Bromonaphthalene-1,4-dione also showed binding ability for Cysteine in bovine serum albumin and could be used as a potential colorimetric sensor among eighteen kinds of natural amino acids. Importantly, the recognition of CySH could be observed by naked eye.

  2. Chemically responsive nanoporous pigments: colorimetric sensor arrays and the identification of aliphatic amines. (United States)

    Bang, Jin Ho; Lim, Sung H; Park, Erwin; Suslick, Kenneth S


    A general method has been developed for the preparation of microspheres of nanoporous pigments, their formulation into chemically responsive pigment inks, and the printing of these inks as colorimetric sensor arrays. Using an ultrasonic-spray aerosol-gel synthesis from chemically responsive dyes and common silica precursors, 16 different nanoporous pigment microspheres have been prepared and characterized. New colorimetric sensor arrays have been created by printing inks of these chemically responsive pigments as primary sensor elements; these arrays have been successfully tested for the detection, identification, and quantitation of toxic aliphatic amines. Among 11 structurally similar amines, complete identification of each analyte without confusion was achieved using hierarchical cluster analysis (HCA). Furthermore, visual identification of ammonia gas was easily made at the IDLH (immediately dangerous to life or health), PEL (permissible exposure limits), and 0.1 PEL concentrations with high reproducibility.

  3. Colorimetric method for the estimation of total limonoid aglycones and glucoside contents in citrus juices. (United States)

    Breksa, Andrew P; Ibarra, Phil


    A method for estimating the total limonoid aglycone and glucoside concentrations in Citrus samples in terms of limonin and limonin glucoside equivalents is presented. The method consists of extraction followed by colorimetric quantification. The colorimetric quantification was based on the formation of red to orange colored derivatives resulting from the treatment of limonin, limonin glucoside, or a fruit extract with 4-(dimethylamino)benzaldehyde (DMAB) in the presence of perchloric and acetic acids. Absorbance maxima for the limonin and limonin glucoside derivatives were found to be 470 and 503 nm, respectively. The influence of DMAB concentration, reaction time, and solvent composition on color development and sensitivity were investigated and optimal assay conditions established. With a microplate format under these conditions, the limits of detection and quantification were determined to be 0.25 and 0.50 microg/mL for limonin and 0.50 and 1.0 microg/mL for limonin glucoside.

  4. Colorimetric detection of trace copper ions based on catalytic leaching of silver-coated gold nanoparticles. (United States)

    Lou, Tingting; Chen, Lingxin; Chen, Zhaopeng; Wang, Yunqing; Chen, Ling; Li, Jinhua


    A colorimetric, label-free, and nonaggregation-based silver coated gold nanoparticles (Ag/Au NPs) probe has been developed for detection of trace Cu(2+) in aqueous solution, based on the fact that Cu(2+) can accelerate the leaching rate of Ag/Au NPs by thiosulfate (S(2)O(3)(2-)). The leaching of Ag/Au NPs would lead to dramatic decrease in the surface plasmon resonance (SPR) absorption as the size of Ag/Au NPs decreased. This colorimetric strategy based on size-dependence of nanoparticles during their leaching process provided a highly sensitive (1.0 nM) and selective detection toward Cu(2+), with a wide linear detection range (5-800 nM) over nearly 3 orders of magnitude. The cost-effective probe allows rapid and sensitive detection of trace Cu(2+) ions in water samples, indicating its potential applicability for the determination of copper in real samples.

  5. An organic indicator functionalized graphene oxide nanocomposite-based colorimetric assay for the detection of sarcosine (United States)

    Xue, Zhonghua; Yin, Bo; Wang, Hui; Li, Mengqian; Rao, Honghong; Liu, Xiuhui; Zhou, Xinbin; Lu, Xiaoquan


    Rapid detection of sarcosine is a key requirement for both diagnosis and treatment of disease. We report here a simple yet sensitive colorimetric nanocomposite platform for rapid detection of sarcosine in alkaline media. The approach exploited the benefits of a rapid color-producing reaction between an organic indicator, 1,2-naphthoquinone-4-sulphonic acid sodium salt (NQS), and the analyte of sarcosine species as well as the good catalytic ability of graphene oxide (GO) to the formation of highly colored products due to its good water dispersibility, extremely large surface area and facile surface modification. As a result, a NQS functionalized GO nanocomposite through π-π stacking has been demonstrated to be useful as a highly efficient catalyst system for the selective and sensitive colorimetric determination of sarcosine by providing a nanocomposite-amplified colorimetric response. Meanwhile, the strategy offered excellent selectivity toward sarcosine species against other amino acids as well as a satisfying detection limit of 0.73 μM. More importantly, by using an electrochemical method, a credible sensing mechanism of GO nanocomposite-based colorimetric platform for a special analyte determination can be easily verified and elucidated, which also provides an attractive alternative to conventional characterization strategies.Rapid detection of sarcosine is a key requirement for both diagnosis and treatment of disease. We report here a simple yet sensitive colorimetric nanocomposite platform for rapid detection of sarcosine in alkaline media. The approach exploited the benefits of a rapid color-producing reaction between an organic indicator, 1,2-naphthoquinone-4-sulphonic acid sodium salt (NQS), and the analyte of sarcosine species as well as the good catalytic ability of graphene oxide (GO) to the formation of highly colored products due to its good water dispersibility, extremely large surface area and facile surface modification. As a result, a NQS

  6. Comparative analysis of colorimetric staining in skin using open-source software. (United States)

    Billings, Paul C; Sanzari, Jenine K; Kennedy, Ann R; Cengel, Keith A; Seykora, John T


    Colorimetric staining techniques such as immunohistochemistry (IHC), immunofluorescence (IF) and histochemistry (HC) provide useful information regarding the localization and relative amount of a molecule/substance in skin. We have developed a novel, straightforward method to assess colorimetric staining by combining features from two open-source software programs. As a proof of principle, we demonstrate the utility of this approach by analysing changes in skin melanin deposition during the radiation-induced tanning response of Yucatan mini-pigs. This method includes a visualization step to validate the accuracy of colour selection before quantitation to ensure accuracy. The data show that this method is robust and will provide a means to obtain accurate comparative analyses of staining in IHC/IF/HC samples.

  7. Design, Certification, and Deployment of the Colorimetric Water Quality Monitoring Kit (CWQMK) (United States)

    Gazda, Daniel B.; Nolan, Daniel J.; Rutz, Jeff A.; Schultz, John R.; Siperko, Lorraine M.; Porter, Marc D.; Lipert, Robert J.; Flint, Stephanie M.; McCoy, J. Torin


    In August 2009, an experimental water quality monitoring kit based on Colorimetric Solid Phase Extraction (CSPE) technology was delivered to the International Space Station (ISS) aboard STS-128/17A. The kit, called the Colorimetric Water Quality Monitoring Kit (CWQMK), was flown and deployed as a Station Development Test Objective (SDTO) experiment on the ISS. The goal of the SDTO experiment is to evaluate the acceptability of CSPE technology for routine water quality monitoring on the ISS. This paper provides an overview of the SDTO experiment, as well as a detailed description of the CWQMK hardware and a summary of the testing and analysis conducted to certify the CWQMK for use on the ISS. The initial results obtained from the SDTO experiment are also reported and discussed in detail

  8. Discrimination of honeys using colorimetric sensor arrays, sensory analysis and gas chromatography techniques. (United States)

    Tahir, Haroon Elrasheid; Xiaobo, Zou; Xiaowei, Huang; Jiyong, Shi; Mariod, Abdalbasit Adam


    Aroma profiles of six honey varieties of different botanical origins were investigated using colorimetric sensor array, gas chromatography-mass spectrometry (GC-MS) and descriptive sensory analysis. Fifty-eight aroma compounds were identified, including 2 norisoprenoids, 5 hydrocarbons, 4 terpenes, 6 phenols, 7 ketones, 9 acids, 12 aldehydes and 13 alcohols. Twenty abundant or active compounds were chosen as key compounds to characterize honey aroma. Discrimination of the honeys was subsequently implemented using multivariate analysis, including hierarchical clustering analysis (HCA) and principal component analysis (PCA). Honeys of the same botanical origin were grouped together in the PCA score plot and HCA dendrogram. SPME-GC/MS and colorimetric sensor array were able to discriminate the honeys effectively with the advantages of being rapid, simple and low-cost. Moreover, partial least squares regression (PLSR) was applied to indicate the relationship between sensory descriptors and aroma compounds.

  9. Mercury CEM Calibration

    Energy Technology Data Exchange (ETDEWEB)

    John Schabron; Joseph Rovani; Mark Sanderson


    Mercury continuous emissions monitoring systems (CEMS) are being implemented in over 800 coal-fired power plant stacks. The power industry desires to conduct at least a full year of monitoring before the formal monitoring and reporting requirement begins on January 1, 2009. It is important for the industry to have available reliable, turnkey equipment from CEM vendors. Western Research Institute (WRI) is working closely with the Electric Power Research Institute (EPRI), the National Institute of Standards and Technology (NIST), and the Environmental Protection Agency (EPA) to facilitate the development of the experimental criteria for a NIST traceability protocol for dynamic elemental mercury vapor generators. The generators are used to calibrate mercury CEMs at power plant sites. The Clean Air Mercury Rule (CAMR) which was published in the Federal Register on May 18, 2005 requires that calibration be performed with NIST-traceable standards (Federal Register 2007). Traceability procedures will be defined by EPA. An initial draft traceability protocol was issued by EPA in May 2007 for comment. In August 2007, EPA issued an interim traceability protocol for elemental mercury generators (EPA 2007). The protocol is based on the actual analysis of the output of each calibration unit at several concentration levels ranging initially from about 2-40 {micro}g/m{sup 3} elemental mercury, and in the future down to 0.2 {micro}g/m{sup 3}, and this analysis will be directly traceable to analyses by NIST. The document is divided into two separate sections. The first deals with the qualification of generators by the vendors for use in mercury CEM calibration. The second describes the procedure that the vendors must use to certify the generator models that meet the qualification specifications. The NIST traceable certification is performance based, traceable to analysis using isotope dilution inductively coupled plasma/mass spectrometry performed by NIST in Gaithersburg, MD. The

  10. Colorimetric assessment of BCR-ABL1 transcripts in clinical samples via gold nanoprobes. (United States)

    Vinhas, Raquel; Correia, Cláudia; Ribeiro, Patricia; Lourenço, Alexandra; Botelho de Sousa, Aida; Fernandes, Alexandra R; Baptista, Pedro V


    Gold nanoparticles functionalized with thiolated oligonucleotides (Au-nanoprobes) have been used in a range of applications for the detection of bioanalytes of interest, from ions to proteins and DNA targets. These detection strategies are based on the unique optical properties of gold nanoparticles, in particular, the intense color that is subject to modulation by modification of the medium dieletric. Au-nanoprobes have been applied for the detection and characterization of specific DNA sequences of interest, namely pathogens and disease biomarkers. Nevertheless, despite its relevance, only a few reports exist on the detection of RNA targets. Among these strategies, the colorimetric detection of DNA has been proven to work for several different targets in controlled samples but demonstration in real clinical bioanalysis has been elusive. Here, we used a colorimetric method based on Au-nanoprobes for the direct detection of the e14a2 BCR-ABL fusion transcript in myeloid leukemia patient samples without the need for retro-transcription. Au-nanoprobes directly assessed total RNA from 38 clinical samples, and results were validated against reverse transcription-nested polymerase chain reaction (RT-nested PCR) and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The colorimetric Au-nanoprobe assay is a simple yet reliable strategy to scrutinize myeloid leukemia patients at diagnosis and evaluate progression, with obvious advantages in terms of time and cost, particularly in low- to medium-income countries where molecular screening is not routinely feasible. Graphical abstract Gold nanoprobe for colorimetric detection of BCR-ABL1 fusion transcripts originating from the Philadelphia chromosome.

  11. Selective Colorimetric Fluoride Sensor: A Heteroditopic Re-ceptor Combining Pyrrolic Amide with Urea

    Institute of Scientific and Technical Information of China (English)

    YIN Zhenming; LIU Shangyuan


    A heteroditopic anion receptor, beating one pyrrolic amide site and a urea site, has been synthesized. UV-Vis spectrum studies in CH3CN solution revealed that the receptor had higher anion binding ability than the homoditopic one. A naked-eye detectable color change, from colorless to yellow, of the receptor solution took place when fluoride was added, which indicates that the receptor has potential application to selective colorimetric fluo-ride sensing.

  12. Ultrasensitive colorimetric detection of Cu2+ ion based on catalytic oxidation of L-cysteine. (United States)

    Yin, Kun; Li, Bowei; Wang, Xiaochun; Zhang, Weiwei; Chen, Lingxin


    As an essential element, copper ion (Cu(2+)) plays important roles in human beings for its participation in diverse metabolic processes as a cofactor and/or a structural component of enzymes. However, excessive uptake of Cu(2+) ion gives rise to the risk of certain diseases. So, it is important to develop simple ways to monitor and detect Cu(2+) ion. In this study, a simple, facile colorimetric sensor for the ultrasensitive determination of Cu(2+) ion was developed based on the following principle: L-cysteine and 1-chloro-2,4-dinitrobenzene (CDNB) could be conjugated to form the yellow product 2,4-dinitrophenylcysteine (DNPC), which was measurable at 355nm; however, upon addition of Cu(2+) ion, the absorbance of DNPC would be decreased owing to the Cu(2+) ion catalytic oxidation of L-cysteine to L-cystine in the presence of O2. Thus, the colorimetric detection of Cu(2+) ion could be achieved. The optimal pH, buffer, temperature and incubation time for the colorimetric sensor were obtained of pH 6.8 in 0.1M HEPES solution, 90 °C and 50 min, respectively. A good linearity within the range of 0.8-10 nM (r = 0.996) was attained, with a high detectability up to 0.5nM. Analyses of Cu(2+) ion in drinking water, lake water, seawater and biological samples were carried out and the method performances were found to agree well with that obtained by ICP-MS. The developed simple colorimetric sensor proved applicable for Cu(2+) ion determination in real samples with high sensitivity and selectivity.

  13. Upconversion ratiometric fluorescence and colorimetric dual-readout assay for uric acid. (United States)

    Fang, Aijin; Wu, Qiongqiong; Lu, Qiujun; Chen, Hongyu; Li, Haitao; Liu, Meiling; Zhang, Youyu; Yao, Shouzhuo


    A new upconversion colorimetric and ratiometric fluorescence detection method for uric acid (UA) has been designed. Yb(3+), Er(3+) and Tm(3+) co-doped NaYF4 nanoparticles (UCNPs) was synthesized. The co-doped NaYF4 nanoparticles, emit upconversion fluorescence with four typical emission peaks centered at 490nm, 557nm, 670nm and 705nm under the 980nm near-infrared (NIR) irradiation. The ZnFe2O4 magnetic nanoparticles (MNPs) possessing excellent peroxidase-like activity was prepared and used to catalyze oxidation the coupling of N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt (TOPS) and 4-amino-antipyrine (4-AAP) in the presence of H2O2 to form purple products (compound 1) which has a characteristic absorption peak located at 550nm. The upconversion fluorescence at 557nm was quenched by the compound 1 while the upconversion emission at 705nm was essentially unchanged, the fluorescence ratio ((I557/I705)0/(I557/I705)) is positively proportional to UA concentration in existence of uricase. More importantly, colorimetric signal can be easily observed and applied to directly distinguish the concentration of UA by the naked eye. Under the optimized conditions, the linear range of colorimetric and ratiometric fluorescence sensing towards UA was 0.01-1mM, the detection limits were as low as 5.79μM and 2.86μM (S/N=3), respectively. The proposed method has been successfully applied to the analysis of UA in human serum. These results indicate that the colorimetric and ratiometric fluorescence dual-readout assay method has great potential for applications in physiological and pathological diagnosis.

  14. Magnetic bead-based reverse colorimetric immunoassay strategy for sensing biomolecules. (United States)

    Gao, Zhuangqiang; Xu, Mingdi; Hou, Li; Chen, Guonan; Tang, Dianping


    A novel reverse colorimetric immunoassay (RCIA) strategy was for the first time designed and utilized for sensitive detection of low-abundance protein (prostate-specific antigen, PSA, used in this case) in biological fluids by coupling highly catalytic efficient catalase with magnetic bead-based peroxidase mimics. To construct such a RCIA system, two nanostructures including magnetic beads and gold nanoparticles were first synthesized and functionalized with anti-PSA capture antibody and catalase/anti-PSA detection antibody, respectively. Thereafter, a specific sandwich-type immunoassay format was employed for determination of PSA by using functional gold nanoparticles as enzymatic bioreactors and anti-PSA-conjugated magnetic beads as a colorimetric developer. The carried catalase, followed by the sandwiched immunocomplex, partially consumed the added hydrogen peroxide in the detection solution, which slowed down the catalytic efficiency of magnetic bead-based peroxidase mimics toward TMB/H2O2, thereby weakening the visible color and decreasing the colorimetric density. Different from conventional colorimetric immunoassay, the RCIA method determined the residual hydrogen peroxide in the substrate after consumption. Under the optimal conditions, the developed RCIA exhibited a wide dynamic range of 0.05-20 ng mL(-1) toward PSA with a detection limit of 0.03 ng mL(-1) at the 3Sblank level. Intra- and interassay coefficients of variation were below 6.1% and 9.3%, respectively. Additionally, the methodology was further validated for the analysis of 12 PSA clinical serum specimens, giving results in good accordance with those obtained by the commercially available enzyme-linked immunosorbent assay (ELISA) method.

  15. Electrochemical and colorimetric assessment on the influence of target metals on wine color



    Presentado en Book of abstracts of the11th International Conference on Electroanalysis ESEAC, 2006; P2-081. Three year old samples of Vitis vinifera origin-controlled red wine samples were spiked with adequate amounts of metals and subsequent colorimetric parameters evolution and complexing capacity behaviour were checked. The used approach consisted in the study of the complexing capacity of natural occurring ligands on wine with respect to Zn and Cu by means of stripping voltammetry in ...

  16. Visual and colorimetric methods for rapid determination of total tannins in vegetable raw materials

    Directory of Open Access Journals (Sweden)

    S. P. Kalinkina


    Full Text Available The article is dedicated to the development of rapid colorimetric method for determining the amount of tannins in aqueous extracts of vegetable raw materials. The sorption-based colorimetric test is determining sorption tannins polyurethane foam, impregnated of FeCl3, receiving on its surface painted in black and green color of the reaction products and the determination of their in sorbent matrix. Selectivity is achieved by determining the tannins specific interaction of polyphenols with iron ions (III. The conditions of sorption-colorimetric method: the concentration of ferric chloride (III, impregnated in the polyurethane foam; sorbent mass in the analytical cartridge; degree of loading his agent; the contact time of the phases. color scales have been developed for the visual determination of the amount of tannins in terms of gallic acid. Spend a digitized image obtained scales using computer program “Sorbfil TLC”, excluding a subjective assessment of the intensity of the color scale of the test. The results obtained determine the amount of tannins in aqueous extracts of vegetable raw rapid method using tablets and analytical cartridges. The results of the test determination of tannins with visual and densitometric analytical signal registration are compared to known methods. Spend a metrological evaluation of the results of determining the amount of tannins sorption rapid colorimetric methods. Time visual and densitometric rapid determination of tannins, taking into account the sample preparation is 25–30 minutes, the relative error does not exceed 28 %. The developed test methods for quantifying the content of tannins allow to exclude the use of sophisticated analytical equipment, carry out the analysis in non-laboratory conditions do not require highly skilled personnel.

  17. Metal complex with terpyrindine derivative ligand as highly selective colorimetric sensor for iron(Ⅲ)

    Institute of Scientific and Technical Information of China (English)

    Peng Wang; Taka-aki Okamura; Hong-Ping Zhou; Wei-Yin Sun; Yu-Peng Tian


    A new metal complex [MnL2](NO3)2.CH3CN (1) was synthesized by reaction of 4'-4-(1,2,4-triazol-1-yl)-phenyl-2,2':6',2"-terpyridine (L) with manganese nitrate.The structure of the complex was determined by X-ray crystallography.The results of UV-vis studies showed that the complex exhibits colorimetric sensing ability for Fe3+,which can be observed by naked eye.

  18. Automatic Fixture Planning

    Institute of Scientific and Technical Information of China (English)


    Fixture planning is a crucial problem in the field of fixture design. In this paper, the research scope and research methods of the computer-aided fixture planning are presented. Based on positioning principles of typical workparts, an ANN algorithm, namely Hopfield algorithm, is adopted for the automatic fixture planning. Also, this paper leads a deep research into the selection of positioning and clamping surfaces (or points) on workparts using positioning-clamping-surface-selecting rules and matrix evaluation of deterministic workpart positioning. In the end of this paper, the methods to select positioning and clamping elements from database and the layout algorithm to assemble the selected fixture elements into a tangible fixture are developed.

  19. A Universal Fast Colorimetric Method for DNA Signal Detection with DNA Strand Displacement and Gold Nanoparticles

    Directory of Open Access Journals (Sweden)

    Xin Li


    Full Text Available DNA or gene signal detection is of great significance in many fields including medical examination, intracellular molecular monitoring, and gene disease signal diagnosis, but detection of DNA or gene signals in a low concentration with instant visual results remains a challenge. In this work, a universal fast and visual colorimetric detection method for DNA signals is proposed. Specifically, a DNA signal amplification “circuit” based on DNA strand displacement is firstly designed to amplify the target DNA signals, and then thiol modified hairpin DNA strands and gold nanoparticles are used to make signal detection results visualized in a colorimetric manner. If the target DNA signal exists, the gold nanoparticles aggregate and settle down with color changing from dark red to grey quickly; otherwise, the gold nanoparticles’ colloids remain stable in dark red. The proposed method provides a novel way to detect quickly DNA or gene signals in low concentrations with instant visual results. When applied in real-life, it may provide a universal colorimetric method for gene disease signal diagnosis.

  20. Colorimetric determination of hypochlorite with unmodified gold nanoparticles through the oxidation of a stabilizer thiol compound. (United States)

    Zhang, Jia; Wang, Xiaolei; Yang, Xiurong


    In this article, we report a colorimetric approach for the determination of hypochlorite (OCl(-)) with gold nanoparticles (Au NPs). The test proceeds as two individual steps and selectivity is developed based on the strong oxidizing ability of hypochlorite. In concentrated phosphate buffer (PB), the red solution of citrate-capped Au NPs could be stabilized by the chemisorption of 11-mercaptoundecanoic acid (MUA), without which the colloidal suspension turned blue because of salt-induced particles aggregation. However, by its oxidizing power, OCl(-) converted the alkanethiol to a sulfonate derivative, which could not protect Au NPs from aggregation, thereby a blue solution was observed after the subsequent addition of Au suspension. With this method and under the optimal conditions (28 nm Au NP, 50 mM PB, pH 7.0, and 10 min for the colorimetric response), 1.5 μM of OCl(-) can be easily visualized by the naked eye. This sensitive and selective colorimetric assay opens up a fresh insight of facile, rapid, and reliable detection of OCl(-), and may find its future application in the monitoring of OCl(-)/HOCl in waters sanitized by chlorine or hypochlorite compounds.

  1. Multispectral image compression methods for improvement of both colorimetric and spectral accuracy (United States)

    Liang, Wei; Zeng, Ping; Xiao, Zhaolin; Xie, Kun


    We propose that both colorimetric and spectral distortion in compressed multispectral images can be reduced by a composite model, named OLCP(W)-X (OptimalLeaders_Color clustering-PCA-W weighted-X coding). In the model, first the spectral-colorimetric clustering is designed for sparse equivalent representation by generating spatial basis. Principal component analysis (PCA) is subsequently used in the manipulation of spatial basis for spectral redundancy removal. Then error compensation mechanism is presented to produce predicted difference image, and finally combined with visual characteristic matrix W, and the created image is compressed by traditional multispectral image coding schemes. We introduce four model-based algorithms to explain their validity. The first two algorithms are OLCPWKWS (OLC-PCA-W-KLT-WT-SPIHT) and OLCPKWS, in which Karhunen-Loeve transform, wavelet transform, and set partitioning in hierarchical trees coding are applied for the created image compression. And the latter two methods are OLCPW-JPEG2000-MCT and OLCP-JPEG2000-MCT. Experimental results show that, compared with the corresponding traditional coding, the proposed OLCPW-X schemes can significantly improve the colorimetric accuracy of rebuilding images under various illumination conditions and generally achieve satisfactory peak signal-to-noise ratio under the same compression ratio. And OLCP-X methods could always ensure superior spectrum reconstruction. Furthermore, our model has excellent performance on user interaction.

  2. Microfluidic chip-based silver nanoparticles aptasensor for colorimetric detection of thrombin. (United States)

    Zhao, Yaju; Liu, Xiaohui; Li, Jie; Qiang, Weibing; Sun, Liang; Li, Hui; Xu, Danke


    In this paper, a colorimetric silver nanoparticles aptasensor (aptamer-AgNPs) was developed for simple and straightforward detection of protein in microfluidic chip. Surface-functionalized microfluidic channels were employed as the capture platform. Then the mixture of target protein and aptamer-AgNPs were injected into the microfluidic channels for colorimetric detection. To demonstrate the performance of this detection platform, thrombin was chosen as a model target protein. Introduction of thrombin could form a sandwich-type complex involving immobilized AgNPs. The amount of aptamer-AgNPs on the complex augmented along with the increase of the thrombin concentration causing different color change that can be analyzed both by naked eyes and a flatbed scanner. This method is featured with low sample consumption, simple processes of microfluidic platform and straightforward colorimetric detection with aptamer-AgNPs. Thrombin at concentrations as low as 20pM can be detected using this aptasensor without signal amplification. This work demonstrated that it had good selectivity over other proteins and it could be a useful strategy to detect other targets with two affinity binding sites for ligands as well.

  3. Colorimetric characteristics of different anatomical sections of muirapiranga (Brosimum sp. wood

    Directory of Open Access Journals (Sweden)

    Claudene Menezes Atayde


    Full Text Available This work aimed to evaluate and compare colorimetric characteristics of three anatomical sections (transverse, tangential and radial of ‘muirapiranga’ bloodwood (Brosimum sp., by using the International Commission on Illumination system, CIELAB 1976 (Comissão Internacional de Iluminantes. By using 10 samples obtained from furniture traders in the municipality of Cruzeiro do Sul (AC, the following colorimetric parameters were determined: lightness (L *, red-green axis (a*, blue-yellow axis (b*, chrome (C and hue angle (h*. An average 34 readings were taken per specimen in each of the planes or sections. The following colorimetric parameters were derived for Brosimum sp. species: L*=42.13, a*=18.87, b*=15.05, C=24.23 and h*=37.86. There were variations in color, indicating significant differences among the three sections in question. As to reflectance, the transverse section (6-28% was found to reflect less light than the tangential (6-43% and radial sections (7-49%. Depending on which anatomical plane is chosen, different shades of red can be obtained, allowing forming similarly shaded wood batches.

  4. Standing-wave resonances in plasmonic nanoumbrella cavities for color generation and colorimetric refractive index sensor (United States)

    Fan, Jiaorong; Li, Zhongyuan; Chen, Zhuojie; Wu, Wengang


    We theoretically investigate the hybridization of the elemental surface plasmons in umbrella-shape plasmonic nanostructures and experimentally demonstrate the implementation of plasmonic multicolor metasurfaces as well as their application in colorimetric sensing. The three-dimension metallic umbrella arrays consist of a periodic canopy-capped-nanopillars with metal-coated sidewall and a backplane metal-film to form vertical nanocavity of canopy and film. Plasmonic coupling and energy confinement in nanocavity induce a noticeably resonance narrowing of multispectral reflection. The metasurfaced nanostructures appeared in vibrant and tunable colors with broad gamut derived from color blending mechanism due to multiple, narrow-band resonances. Vivid colors varied from red, yellow, green, blue to violet are easily achieved. It is also shown that such plasmonic metasurfaces can work as the feasible and real-time colorimetric refractive index sensor by measuring the distinct color variation to glucose concentration changes. Our sensor scheme shows its spectral sensitivity in the periodic umbrella array with respect to the refractive index change to be 242.5 nm/RIU with a figure of merit of 7.3. Furthermore, a refractive index resolution of colorimetric sensing up to 0.025 RIU has been accomplished.

  5. Colorimetric sensing of anions in water using ratiometric indicator-displacement assay. (United States)

    Feng, Liang; Li, Hui; Li, Xiao; Chen, Liang; Shen, Zheng; Guan, Yafeng


    The analysis of anions in water presents a difficult challenge due to their low charge-to-radius ratio, and the ability to discriminate among similar anions often remains problematic. The use of a 3×6 ratiometric indicator-displacement assay (RIDA) array for the colorimetric detection and identification of ten anions in water is reported. The sensor array consists of different combinations of colorimetric indicators and metal cations. The colorimetric indicators chelate with metal cations, forming the color changes. Upon the addition of anions, anions compete with the indicator ligands according to solubility product constants (K(sp)). The indicator-metal chelate compound changes color back dramatically when the competition of anions wins. The color changes of the RIDA array were used as a digital representation of the array response and analyzed with standard statistical methods, including principal component analysis and hierarchical clustering analysis. No confusion or errors in classification by hierarchical clustering analysis were observed in 44 trials. The limit of detection was calculated approximately, and most limits of detections of anions are well below μM level using our RIDA array. The pH effect, temperature influence, interfering anions were also investigated, and the RIDA array shows the feasibility of real sample testing.

  6. Colorimetric and sensory characteristics of fermented cured sausage with Brazilian ostrich meat addition

    Directory of Open Access Journals (Sweden)

    Carlos Pasqualin Cavalheiro


    Full Text Available The aim of this study was to determine the colorimetric and sensory characteristics of a fermented cured sausage containing ostrich meat (Struthio camelus and pork meat. Four treatments were performed: one with no ostrich meat (TC and the others containing 19.08 (T1, 38.34 (T2, and 57.60% (T3 of ostrich meat and pork meat. Colorimetric analyses were measuring L*, a*, b*, C*, and hº. Sensory analysis was conducted assessing color, aroma, flavor, and texture at the end of the sausages' processing. The sausages containing ostrich meat were statistically different from the control in the instrumental colorimetric analysis. In the sensory analysis, no significant differences were observed between the treatments for aroma, flavor, and texture. However, significant differences were found in the color of the sausages due to the high myoglobin content present in the ostrich meat, which resulted in a very dark color in the treatment with the highest percentage of this type of meat.

  7. Variation of phlorotannins among three populations of Fucus vesiculosus as revealed by HPLC and colorimetric quantification. (United States)

    Koivikko, R; Eränen, J K; Loponen, J; Jormalainen, V


    In ecological studies, phlorotannins have conventionally been quantified as a group with similar functionality. Since this group consists of oligo- and polymers, the quantification of their pooled contents alone may not sufficiently describe the variation of these metabolites. Genetic variation, plastic responses to environment, and the ecological functions of separate phlorotannin oligo- and polymers may differ. Two analyses, i.e., the colorimetric Folin-Ciocalteu assay and a normal-phase high-performance liquid chromatographic (HPLC) method were used to study genetic and environmental variation in phlorotannins of the brown alga Fucus vesiculosus (L.). The colorimetric method provides the total phlorotannin content, the latter a profile of 14 separate traces from the phenolic extract that represent an individual or groups of phlorotannins. We reared the algae that originated from three separate populations in a common garden for 3 months under ambient and enriched-nutrient availability and found that they differed in both their total phlorotannin content and in phlorotannin profiles. Some individual traces of the profiles separated the populations more clearly than the colorimetric assay. Although nutrient enrichment decreased total phlorotannin content, it did not show a significant influence on the phlorotannin profile. This implies that plastic responses of compounds other than phlorotannins may interfere with the determination of total phlorotannins. However, the phlorotannin profile and the total content showed genetic variation among local populations of F. vesiculosus; therefore, phlorotannins may respond to natural selection and evolve both quantitatively and qualitatively.

  8. Comparison of macro-gravimetric and micro-colorimetric lipid determination methods. (United States)

    Inouye, Laura S; Lotufo, Guiherme R


    In order to validate a method for lipid analysis of small tissue samples, the standard macro-gravimetric method of Bligh-Dyer (1959) [E.G. Bligh, W.J. Dyer, Can. J. Biochem. Physiol. 37 (1959) 911] and a modification of the micro-colorimetric assay developed by Van Handel (1985) [E. Van Handel, J. Am. Mosq. Control Assoc. 1 (1985) 302] were compared. No significant differences were observed for wet tissues of two species of fish. However, limited analysis of wet tissue of the amphipod, Leptocheirusplumulosus, indicated that the Bligh-Dyer gravimetric method generated higher lipid values, most likely due to the inclusion of non-lipid materials. Additionally, significant differences between the methods were observed with dry tissues, with the micro-colorimetric method consistently reporting calculated lipid values greater than as reported by the gravimetric method. This was most likely due to poor extraction of dry tissue in the standard Bligh-Dyer method, as no significant differences were found when analyzing a single composite extract. The data presented supports the conclusion that the micro-colorimetric method described in this paper is accurate, rapid, and minimizes time and solvent use.

  9. Vertically stacked plasmonic nanoparticles in a circular arrangement: a key to colorimetric refractive index sensing. (United States)

    Seo, Sujin; Gartia, Manas Ranjan; Liu, Gang Logan


    True colorimetric sensing produces a linear spectral response of a single peak within the visible light range with various surrounding media refractive indices. We demonstrate how the circular arrangement of hemispheric silver nanoparticles achieves colorimetric properties without modifying the associated full-width-half-maximum values in a broad range of surrounding media refractive indices. We also show that the vertical out-of-plane arrangement of each circular array in nanoholes enhances the signal-to-noise ratio. High electric field confinement at the interface of the nanoparticles and the supporting substrate reveals the effect of the dielectric constant of the substrate and the morphology of the 3D nanoparticle arrays on achieving a single resonance peak in the visible range with a change in the surrounding refractive index. This study opens up the pathway to top-down fabricated nanostructure platform based plasmonic colorimetric sensing with a single resonance peak in the visible range. The studied rich set of tunable geometrical nanostructures enables broadening of the working optical range of the device.

  10. Evaluation of an end-tidal portable ETCO2 colorimetric breath indicator (COLIBRI). (United States)

    Rabitsch, Werner; Nikolic, Ajsa; Schellongowski, Peter; Kofler, Julia; Kraft, Peter; Krenn, Claus G; Staudinger, Thomas; Locker, Gottfried J; Knöbl, Paul; Hofbauer, Roland; Frass, Michael


    Evaluation of tube position is important after in-hospital and prehospital emergency intubation. Colorimetric breath indicators are devices for immediate control of tube positioning by showing a color change according to end-tidal CO2 (ETCO2) concentrations. We hypothesized that colorimetric breath indicators can yield reliable results for confirmation of tube position. The aim of this study was to evaluate the effectiveness and safety of a new colorimetric breath indicator (Colibri, ICOR AB, Bromma, Sweden) in 147 patients during general anesthesia, in critically ill patients, during transport to in-hospital interventions, and in a study design after insertion of a second tube into the esophagus in long-term ventilated patients. The Colibri was attached between the respective airway and ventilatory tubing. Seventy-three patients were investigated during general anesthesia, 39 patients were observed during long-term ventilation with an average duration of 33 hours, in 15 patients during transport to an intervention for up to 4 hours, and in 20 long-term ventilated patients at the medical intensive-care unit after insertion of a second tube intentionally into the esophagus with the help of a laryngoscope. The Colibri worked well in all groups investigated and showed no false results in the group with tubes inserted into the trachea and esophagus. Data suggest that the Colibri might serve as a valuable tool for evaluating and controlling tube position. This device is independent of power supply or electronic equipment, portable, small, and immediately ready for use.


    Directory of Open Access Journals (Sweden)

    Sezar Gülbaz


    Full Text Available The land development and increase in urbanization in a watershed affect water quantityand water quality. On one hand, urbanization provokes the adjustment of geomorphicstructure of the streams, ultimately raises peak flow rate which causes flood; on theother hand, it diminishes water quality which results in an increase in Total SuspendedSolid (TSS. Consequently, sediment accumulation in downstream of urban areas isobserved which is not preferred for longer life of dams. In order to overcome thesediment accumulation problem in dams, the amount of TSS in streams and inwatersheds should be taken under control. Low Impact Development (LID is a BestManagement Practice (BMP which may be used for this purpose. It is a land planningand engineering design method which is applied in managing storm water runoff inorder to reduce flooding as well as simultaneously improve water quality. LID includestechniques to predict suspended solid loads in surface runoff generated over imperviousurban surfaces. In this study, the impact of LID-BMPs on surface runoff and TSS isinvestigated by employing a calibrated hydrodynamic model for Sazlidere Watershedwhich is located in Istanbul, Turkey. For this purpose, a calibrated hydrodynamicmodel was developed by using Environmental Protection Agency Storm WaterManagement Model (EPA SWMM. For model calibration and validation, we set up arain gauge and a flow meter into the field and obtain rainfall and flow rate data. Andthen, we select several LID types such as retention basins, vegetative swales andpermeable pavement and we obtain their influence on peak flow rate and pollutantbuildup and washoff for TSS. Consequently, we observe the possible effects ofLID on surface runoff and TSS in Sazlidere Watershed.

  12. Spatial calibration of digital scintigraphic images

    Energy Technology Data Exchange (ETDEWEB)

    Esser, P.D.; Seldin, D.W.; Nichols, A.B.; Alderson, P.O.


    Accurate scintigraphic determination of organ dimensions is often difficult because of the wide variety of camera-collimator combinations and display formats used for imaging. An algorithm (SPACE-CAL) was developed for digital spatial calibration of up to six large- or small-field gamma cameras using various collimators and magnification factors. The algorithm employs a master calibration file that can be used at multiple terminals. An interactive subroutine permits the operator to measure distances and areas directly from the images. Evaluation of three operators' measurements of a pine-tree phantom acquired at various magnifications showed that distances could be measured with a high degree of accuracy. Calculation of ejection fraction from end-diastolic and end-systolic areas determined by SPACE-CAL showed a high correlation with automatic count determinations (r=0.96) and angiographic calculations (r=0.93) of ejection fraction in 12 patients. SPACE-CAL provides an easily implemented, reproducible, and rapid method for accurate analysis of organ dimensions in digital images.

  13. ALTEA: The instrument calibration

    Energy Technology Data Exchange (ETDEWEB)

    Zaconte, V. [INFN and University of Rome Tor Vergata, Department of Physics, Via della Ricerca Scientifica 1, 00133 Rome (Italy)], E-mail:; Belli, F.; Bidoli, V.; Casolino, M.; Di Fino, L.; Narici, L.; Picozza, P.; Rinaldi, A. [INFN and University of Rome Tor Vergata, Department of Physics, Via della Ricerca Scientifica 1, 00133 Rome (Italy); Sannita, W.G. [DISM, University of Genova, Genova (Italy); Department of Psychiatry, SUNY, Stoony Brook, NY (United States); Finetti, N.; Nurzia, G.; Rantucci, E.; Scrimaglio, R.; Segreto, E. [Department of Physics, University and INFN, L' Aquila (Italy); Schardt, D. [GSI/Biophysik, Darmstadt (Germany)


    The ALTEA program is an international and multi-disciplinary project aimed at studying particle radiation in space environment and its effects on astronauts' brain functions, as the anomalous perception of light flashes first reported during Apollo missions. The ALTEA space facility includes a 6-silicon telescopes particle detector, and is onboard the International Space Station (ISS) since July 2006. In this paper, the detector calibration at the heavy-ion synchrotron SIS18 at GSI Darmstadt will be presented and compared to the Geant 3 Monte Carlo simulation. Finally, the results of a neural network analysis that was used for ion discrimination on fragmentation data will also be presented.

  14. Dynamic Torque Calibration Unit (United States)

    Agronin, Michael L.; Marchetto, Carl A.


    Proposed dynamic torque calibration unit (DTCU) measures torque in rotary actuator components such as motors, bearings, gear trains, and flex couplings. Unique because designed specifically for testing components under low rates. Measures torque in device under test during controlled steady rotation or oscillation. Rotor oriented vertically, supported by upper angular-contact bearing and lower radial-contact bearing that floats axially to prevent thermal expansion from loading bearings. High-load capacity air bearing available to replace ball bearings when higher load capacity or reduction in rate noise required.

  15. Radiometer Calibration and Characterization (RCC) User's Manual: Windows Version 4.0

    Energy Technology Data Exchange (ETDEWEB)

    Andreas, Afshin M. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Wilcox, Stephen M. [National Renewable Energy Lab. (NREL), Golden, CO (United States)


    The Radiometer Calibration and Characterization (RCC) software is a data acquisition and data archival system for performing Broadband Outdoor Radiometer Calibrations (BORCAL). RCC provides a unique method of calibrating broadband atmospheric longwave and solar shortwave radiometers using techniques that reduce measurement uncertainty and better characterize a radiometer's response profile. The RCC software automatically monitors and controls many of the components that contribute to uncertainty in an instrument's responsivity. This is a user's manual and guide to the RCC software.

  16. A Simple Accelerometer Calibrator (United States)

    Salam, R. A.; Islamy, M. R. F.; Munir, M. M.; Latief, H.; Irsyam, M.; Khairurrijal


    High possibility of earthquake could lead to the high number of victims caused by it. It also can cause other hazards such as tsunami, landslide, etc. In that case it requires a system that can examine the earthquake occurrence. Some possible system to detect earthquake is by creating a vibration sensor system using accelerometer. However, the output of the system is usually put in the form of acceleration data. Therefore, a calibrator system for accelerometer to sense the vibration is needed. In this study, a simple accelerometer calibrator has been developed using 12 V DC motor, optocoupler, Liquid Crystal Display (LCD) and AVR 328 microcontroller as controller system. The system uses the Pulse Wave Modulation (PWM) form microcontroller to control the motor rotational speed as response to vibration frequency. The frequency of vibration was read by optocoupler and then those data was used as feedback to the system. The results show that the systems could control the rotational speed and the vibration frequencies in accordance with the defined PWM.

  17. Adaptive digital calibration techniques for narrow band low-IF receivers with on-chip PLL

    Institute of Scientific and Technical Information of China (English)

    Li Juan; Zhang Huajiang; Zhao Feng; Hong Zhiliang


    Digital calibration and control techniques for narrow band integrated low-IF receivers with on-chip frequency synthesizer are presented. The calibration and control system, which is adopted to ensure an achievable signal-to-noise ratio and bit error rate, consists of a digitally controlled, high resolution dB-linear automatic gain control (AGC), an inphase (I) and quadrature (Q) gain and phase mismatch calibration, and an automatic frequency calibration (AFC) of a wideband voltage-controlled oscillator in a PLL based frequency synthesizer. The calibration system has a low design complexity with little power and small die area. Simulation results show that the calibration system can enlarge the dynamic range to 72 dB and minimize the phase and amplitude imbalance between I and Q to 0.08° and 0.024 dB, respectively, which means the image rejection ratio is better than 60 dB. In addition, the calibration time of the AFC is 1.12μs only with a reference clock of 100 MHz.

  18. Heliostat calibration using attached cameras and artificial targets (United States)

    Burisch, Michael; Sanchez, Marcelino; Olarra, Aitor; Villasante, Cristobal


    The efficiency of the solar field greatly depends on the ability of the heliostats to precisely reflect solar radiation onto a central receiver. To control the heliostats with such a precision requires the accurate knowledge of the motion of each of them. The motion of each heliostat can be described by a set of parameters, most notably the position and axis configuration. These parameters have to be determined individually for each heliostat during a calibration process. With the ongoing development of small sized heliostats, the ability to automatically perform such a calibration becomes more and more crucial as possibly hundreds of thousands of heliostats are involved. Furthermore, efficiency becomes an important factor as small sized heliostats potentially have to be recalibrated far more often, due to the limited stability of the components. In the following we present an automatic calibration procedure using cameras attached to each heliostat which are observing different targets spread throughout the solar field. Based on a number of observations of these targets under different heliostat orientations, the parameters describing the heliostat motion can be estimated with high precision.

  19. Internal Water Vapor Photoacoustic Calibration (United States)

    Pilgrim, Jeffrey S.


    Water vapor absorption is ubiquitous in the infrared wavelength range where photoacoustic trace gas detectors operate. This technique allows for discontinuous wavelength tuning by temperature-jumping a laser diode from one range to another within a time span suitable for photoacoustic calibration. The use of an internal calibration eliminates the need for external calibrated reference gases. Commercial applications include an improvement of photoacoustic spectrometers in all fields of use.

  20. Automatic aircraft recognition (United States)

    Hmam, Hatem; Kim, Jijoong


    Automatic aircraft recognition is very complex because of clutter, shadows, clouds, self-occlusion and degraded imaging conditions. This paper presents an aircraft recognition system, which assumes from the start that the image is possibly degraded, and implements a number of strategies to overcome edge fragmentation and distortion. The current vision system employs a bottom up approach, where recognition begins by locating image primitives (e.g., lines and corners), which are then combined in an incremental fashion into larger sets of line groupings using knowledge about aircraft, as viewed from a generic viewpoint. Knowledge about aircraft is represented in the form of whole/part shape description and the connectedness property, and is embedded in production rules, which primarily aim at finding instances of the aircraft parts in the image and checking the connectedness property between the parts. Once a match is found, a confidence score is assigned and as evidence in support of an aircraft interpretation is accumulated, the score is increased proportionally. Finally a selection of the resulting image interpretations with the highest scores, is subjected to competition tests, and only non-ambiguous interpretations are allowed to survive. Experimental results demonstrating the effectiveness of the current recognition system are given.

  1. Automatic Kurdish Dialects Identification

    Directory of Open Access Journals (Sweden)

    Hossein Hassani


    Full Text Available Automatic dialect identification is a necessary Lan guage Technology for processing multi- dialect languages in which the dialects are linguis tically far from each other. Particularly, this becomes crucial where the dialects are mutually uni ntelligible. Therefore, to perform computational activities on these languages, the sy stem needs to identify the dialect that is the subject of the process. Kurdish language encompasse s various dialects. It is written using several different scripts. The language lacks of a standard orthography. This situation makes the Kurdish dialectal identification more interesti ng and required, both form the research and from the application perspectives. In this research , we have applied a classification method, based on supervised machine learning, to identify t he dialects of the Kurdish texts. The research has focused on two widely spoken and most dominant Kurdish dialects, namely, Kurmanji and Sorani. The approach could be applied to the other Kurdish dialects as well. The method is also applicable to the languages which are similar to Ku rdish in their dialectal diversity and differences.

  2. Field calibration of cup anemometers

    DEFF Research Database (Denmark)

    Kristensen, L.; Jensen, G.; Hansen, A.


    An outdoor calibration facility for cup anemometers, where the signals from 10 anemometers of which at least one is a reference can be can be recorded simultaneously, has been established. The results are discussed with special emphasis on the statisticalsignificance of the calibration expressions....... It is concluded that the method has the advantage that many anemometers can be calibrated accurately with a minimum of work and cost. The obvious disadvantage is that the calibration of a set of anemometersmay take more than one month in order to have wind speeds covering a sufficiently large magnitude range...

  3. Radiological Calibration and Standards Facility (United States)

    Federal Laboratory Consortium — PNNL maintains a state-of-the-art Radiological Calibration and Standards Laboratory on the Hanford Site at Richland, Washington. Laboratory staff provide expertise...

  4. SURF Model Calibration Strategy

    Energy Technology Data Exchange (ETDEWEB)

    Menikoff, Ralph [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)


    SURF and SURFplus are high explosive reactive burn models for shock initiation and propagation of detonation waves. They are engineering models motivated by the ignition & growth concept of high spots and for SURFplus a second slow reaction for the energy release from carbon clustering. A key feature of the SURF model is that there is a partial decoupling between model parameters and detonation properties. This enables reduced sets of independent parameters to be calibrated sequentially for the initiation and propagation regimes. Here we focus on a methodology for tting the initiation parameters to Pop plot data based on 1-D simulations to compute a numerical Pop plot. In addition, the strategy for tting the remaining parameters for the propagation regime and failure diameter is discussed.

  5. RX130 Robot Calibration (United States)

    Fugal, Mario


    In order to create precision magnets for an experiment at Oak Ridge National Laboratory, a new reverse engineering method has been proposed that uses the magnetic scalar potential to solve for the currents necessary to produce the desired field. To make the magnet it is proposed to use a copper coated G10 form, upon which a drill, mounted on a robotic arm, will carve wires. The accuracy required in the manufacturing of the wires exceeds nominal robot capabilities. However, due to the rigidity as well as the precision servo motor and harmonic gear drivers, there are robots capable of meeting this requirement with proper calibration. Improving the accuracy of an RX130 to be within 35 microns (the accuracy necessary of the wires) is the goal of this project. Using feedback from a displacement sensor, or camera and inverse kinematics it is possible to achieve this accuracy.

  6. Electronic amplifiers for automatic compensators

    CERN Document Server

    Polonnikov, D Ye


    Electronic Amplifiers for Automatic Compensators presents the design and operation of electronic amplifiers for use in automatic control and measuring systems. This book is composed of eight chapters that consider the problems of constructing input and output circuits of amplifiers, suppression of interference and ensuring high sensitivity.This work begins with a survey of the operating principles of electronic amplifiers in automatic compensator systems. The succeeding chapters deal with circuit selection and the calculation and determination of the principal characteristics of amplifiers, as

  7. The Automatic Telescope Network (ATN)

    CERN Document Server

    Mattox, J R


    Because of the scheduled GLAST mission by NASA, there is strong scientific justification for preparation for very extensive blazar monitoring in the optical bands to exploit the opportunity to learn about blazars through the correlation of variability of the gamma-ray flux with flux at lower frequencies. Current optical facilities do not provide the required capability.Developments in technology have enabled astronomers to readily deploy automatic telescopes. The effort to create an Automatic Telescope Network (ATN) for blazar monitoring in the GLAST era is described. Other scientific applications of the networks of automatic telescopes are discussed. The potential of the ATN for science education is also discussed.

  8. Calibration of Nanopositioning Stages

    Directory of Open Access Journals (Sweden)

    Ning Tan


    Full Text Available Accuracy is one of the most important criteria for the performance evaluation of micro- and nanorobots or systems. Nanopositioning stages are used to achieve the high positioning resolution and accuracy for a wide and growing scope of applications. However, their positioning accuracy and repeatability are not well known and difficult to guarantee, which induces many drawbacks for many applications. For example, in the mechanical characterisation of biological samples, it is difficult to perform several cycles in a repeatable way so as not to induce negative influences on the study. It also prevents one from controlling accurately a tool with respect to a sample without adding additional sensors for closed loop control. This paper aims at quantifying the positioning repeatability and accuracy based on the ISO 9283:1998 standard, and analyzing factors influencing positioning accuracy onto a case study of 1-DoF (Degree-of-Freedom nanopositioning stage. The influence of thermal drift is notably quantified. Performances improvement of the nanopositioning stage are then investigated through robot calibration (i.e., open-loop approach. Two models (static and adaptive models are proposed to compensate for both geometric errors and thermal drift. Validation experiments are conducted over a long period (several days showing that the accuracy of the stage is improved from typical micrometer range to 400 nm using the static model and even down to 100 nm using the adaptive model. In addition, we extend the 1-DoF calibration to multi-DoF with a case study of a 2-DoF nanopositioning robot. Results demonstrate that the model efficiently improved the 2D accuracy from 1400 nm to 200 nm.

  9. Automatic Data Normalization and Parameterization for Optical Motion Tracking

    Directory of Open Access Journals (Sweden)

    Leif Kobbelt


    Full Text Available Methods for optical motion capture often require time-consuming manual processing before the data can be used for subsequent tasks such as retargeting or character animation. These processing steps restrict the applicability of motion capturing especially for dynamic VR-environments with real time requirements. To solve these problems, we present two additional, fast and automatic processing stages based on our motion capture pipeline presented in [ HSK05 ]. A normalization step aligns the recorded coordinate systems with the skeleton structure to yield a common and intuitive data basis across different recording sessions. A second step computes a parameterization based on automatically extracted main movement axes to generate a compact motion description. Our method does not restrict the placement of marker bodies nor the recording setup, and only requires a short calibration phase.

  10. Research on artificial neural network for LCD colorimetric characterization%人工神经网络法LCD色度特性化研究

    Institute of Scientific and Technical Information of China (English)

    张继艳; 熊飞兵


    Now there are many research methods for LCD Colorimetric Characterization at home and abroad,we use artificial neural networks to describe the color characteristics of LCD monitors quantitatively. Compared to S-shape model, we analyze the advantages and disadvantages of the two methods on the basis of experimental measurements. The neural network training method has been mainly studied to see how the transfer function, hidden layers and the number of the hidden units influence on the results. And finally we get the artificial neural network color calibration model.%目前国内外LCD的特性化研究方法已经很多.本文采用人工神经网络法定量描述了液晶显示器的颜色特性.对比S-shape模型,在实验测量的基础上,比较了两种方法的优缺点.神经网络主要研究了训练方法、传递函数、隐层数和隐层单元数对结果的影响,最后得到人工神经网络法的颜色校正模型.

  11. Cost-effective and sensitive colorimetric immunosensing using an iron oxide-to-Prussian blue nanoparticle conversion strategy. (United States)

    Fu, Guanglei; Sanjay, Sharma T; Li, XiuJun


    The development of new sensitive, cost-effective and user-friendly colorimetric bioassays is in increasing demand to meet the requirement of modern clinical diagnostics and field detection. Herein, a novel iron oxide-to-Prussian blue (PB) nanoparticle (NP) conversion strategy was developed and applied to sensitive colorimetric immunosensing of cancer biomarkers. In a typical sandwich-type immunosensing system, the captured spherical antibody-conjugated iron oxide NPs were transformed into cubic PB NPs, which exhibited a highly visible blue color with high molar extinction coefficients. Hence, a new colorimetric immunosensing strategy was developed as a result of this low cost and simple transformation process. Without the aid of any complex nanoparticle stabilizing ligands and signal amplification processes, prostate-specific antigen as a model analyte can be detected at a concentration as low as 1.0 ng mL(-1) by the naked eye with good reliability for detection of real human serum samples. This is the first attempt to develop and apply the iron oxide-to-PB NP colorimetric conversion strategy for immunosensing, and shows great promise for the development of new sensitive, cost-effective and user-friendly colorimetric bioassays in various bioanalytical applications, especially in low-resource settings.

  12. Gold-nanorod-based colorimetric and fluorescent approach for sensitive and specific assay of disease-related gene and mutation. (United States)

    Wang, Wenhong; Zhao, Yina; Jin, Yan


    Sensitive and specific detection of disease-related gene and single nucleotide polymorphism (SNP) is of great importance in cancer diagnosis. Here, a colorimetric and fluorescent approach is described for detection of the p53 gene and SNP in homogeneous solution by using gold nanorods (GNRs) as both colorimetric probe and fluorescence quencher. Hairpin oligonucleotide was utilized as DNA probe to ensure highly sequence-specific detection of target DNA. In the presence of target DNA, the formation of DNA duplex greatly changed the electrostatic interaction between GNR and DNAs, leading to an obvious change in fluorescence and colorimetric response. The detection limit of fluorescent and colorimetric assay is 0.26 pM and 0.3 nM, respectively. Both fluorescence and colorimetric strategies were able to effectively discriminate complementary DNA from single-base mismatched DNA, which is meaningful for cancer diagnosis. More important, target DNA can be detected as low as 10 nM by the naked eye. Furthermore, transmission electron microscopy and fluorescence anisotropy measurements demonstrated that the color change as well as fluorescence quenching is ascribed to the DNA hybridization-induced aggregation of GNRs. Therefore, the assay provided a fast, sensitive, cost-effective, and specific sensing platform for detecting disease-related gene and SNP.

  13. Clothes Dryer Automatic Termination Evaluation

    Energy Technology Data Exchange (ETDEWEB)

    TeGrotenhuis, Ward E.


    Volume 2: Improved Sensor and Control Designs Many residential clothes dryers on the market today provide automatic cycles that are intended to stop when the clothes are dry, as determined by the final remaining moisture content (RMC). However, testing of automatic termination cycles has shown that many dryers are susceptible to over-drying of loads, leading to excess energy consumption. In particular, tests performed using the DOE Test Procedure in Appendix D2 of 10 CFR 430 subpart B have shown that as much as 62% of the energy used in a cycle may be from over-drying. Volume 1 of this report shows an average of 20% excess energy from over-drying when running automatic cycles with various load compositions and dryer settings. Consequently, improving automatic termination sensors and algorithms has the potential for substantial energy savings in the U.S.

  14. Automatic Coarse Graining of Polymers


    Faller, Roland


    Several recently proposed semi--automatic and fully--automatic coarse--graining schemes for polymer simulations are discussed. All these techniques derive effective potentials for multi--atom units or super--atoms from atomistic simulations. These include techniques relying on single chain simulations in vacuum and self--consistent optimizations from the melt like the simplex method and the inverted Boltzmann method. The focus is on matching the polymer structure on different scales. Several ...

  15. Automatic Sarcasm Detection: A Survey


    Joshi, Aditya; Bhattacharyya, Pushpak; Carman, Mark James


    Automatic sarcasm detection is the task of predicting sarcasm in text. This is a crucial step to sentiment analysis, considering prevalence and challenges of sarcasm in sentiment-bearing text. Beginning with an approach that used speech-based features, sarcasm detection has witnessed great interest from the sentiment analysis community. This paper is the first known compilation of past work in automatic sarcasm detection. We observe three milestones in the research so far: semi-supervised pat...

  16. Prospects for de-automatization. (United States)

    Kihlstrom, John F


    Research by Raz and his associates has repeatedly found that suggestions for hypnotic agnosia, administered to highly hypnotizable subjects, reduce or even eliminate Stroop interference. The present paper sought unsuccessfully to extend these findings to negative priming in the Stroop task. Nevertheless, the reduction of Stroop interference has broad theoretical implications, both for our understanding of automaticity and for the prospect of de-automatizing cognition in meditation and other altered states of consciousness.

  17. The automatization of journalistic narrative

    Directory of Open Access Journals (Sweden)

    Naara Normande


    Full Text Available This paper proposes an initial discussion about the production of automatized journalistic narratives. Despite being a topic discussed in specialized sites and international conferences in communication area, the concepts are still deficient in academic research. For this article, we studied the concepts of narrative, databases and algorithms, indicating a theoretical trend that explains this automatized journalistic narratives. As characterization, we use the cases of Los Angeles Times, Narrative Science and Automated Insights.

  18. Process automatization in system administration


    Petauer, Janja


    The aim of the thesis is to present automatization of user management in company Studio Moderna. The company has grown exponentially in recent years, that is why we needed to find faster, easier and cheaper way of man- aging user accounts. We automatized processes of creating, changing and removing user accounts within Active Directory. We prepared user interface inside of existing application, used Java Script for drop down menus, wrote script in scripting programming langu...

  19. Research on colorimetric measurement for cotton high volume instrument%大容量棉花纤维测试仪的色泽测量研究

    Institute of Scientific and Technical Information of China (English)

    叶炜; 叶关荣; 肖中高; 毛滋春


    简要回顾了大容量棉花纤维测试仪及棉花色泽测量的发展历史.在长期跟踪国外色泽测量技术的基础上,研制了采用45/0照明与观察几何条件、瞄准式亮度传感器并结合光谱分析系统,以及符合CIE规范的标准色板光谱反射比测试系统.基于该系统所标定的色板对色泽仪进行校准,并采用全数字色度检测技术结合尼克松-亨特色度空间,研制完成基于单色板标定的棉花色泽测量仪器并完成对棉花的反射比和黄度测试.该棉花色泽仪在稳定性、台差、校准板测试以及对美国农业部标准棉样的测试中,其测试精度接近进口色泽仪测量水平.%Cotton high volume instrument ( HVI) and cotton colorimeter have been briefly reviewed firstly. Based on continuous tracking of the measurement technique of international cotton colorimeter, a spectral reflectance measure-ment system with standard white tile is developed, which adopts spot-luminance sensor, optical system of 4S/0 standard illumination and viewing condition combined with spectrophotometer, and accords with CIE regulation. Fur-thermore , a cotton colorimeter based on single standard color tile calibration is developed using all digital colorimet-ric measurement technique and the computation method of Nickerson-Hunter color space, and the cotton colorimeter is calibrated based on the standard tile calibrated using the system. Cotton reflectance and yellowness measurements were performed using the developed instrument, and evaluation results show that the measurement accuracy of the cotton colorimeter is close to that of the colorimeter of foreign HVI in stability, discrepancy between instruments, measurement of calibrated tile and measurement of standard cotton sample from USDA.

  20. Tectonic calibrations in molecular dating

    Institute of Scientific and Technical Information of China (English)



    Molecular dating techniques require the use of calibrations, which are usually fossil or geological vicariance-based.Fossil calibrations have been criticised because they result only in minimum age estimates. Based on a historical biogeographic perspective, Ⅰ suggest that vicariance-based calibrations are more dangerous. Almost all analytical methods in historical biogeography are strongly biased towards inferring vicariance, hence vicariance identified through such methods is unreliable. Other studies, especially of groups found on Gondwanan fragments, have simply assumed vicariance. Although it was previously believed that vicariance was the predominant mode of speciation, mounting evidence now indicates that speciation by dispersal is common, dominating vicariance in several groups. Moreover, the possibility of speciation having occurred before the said geological event cannot be precluded. Thus, geological calibrations can under- or overestimate times, whereas fossil calibrations always result in minimum estimates. Another major drawback of vicariant calibrations is the problem of circular reasoning when the resulting estimates are used to infer ages of biogeographic events. Ⅰ argue that fossil-based dating is a superior alternative to vicariance, primarily because the strongest assumption in the latter, that speciation was caused by the said geological process, is more often than not the most tenuous. When authors prefer to use a combination of fossil and vicariant calibrations, one suggestion is to report results both with and without inclusion of the geological constraints. Relying solely on vicariant calibrations should be strictly avoided.

  1. Towards automatic classification of all WISE sources (United States)

    Kurcz, A.; Bilicki, M.; Solarz, A.; Krupa, M.; Pollo, A.; Małek, K.


    Context. The Wide-field Infrared Survey Explorer (WISE) has detected hundreds of millions of sources over the entire sky. Classifying them reliably is, however, a challenging task owing to degeneracies in WISE multicolour space and low levels of detection in its two longest-wavelength bandpasses. Simple colour cuts are often not sufficient; for satisfactory levels of completeness and purity, more sophisticated classification methods are needed. Aims: Here we aim to obtain comprehensive and reliable star, galaxy, and quasar catalogues based on automatic source classification in full-sky WISE data. This means that the final classification will employ only parameters available from WISE itself, in particular those which are reliably measured for the majority of sources. Methods: For the automatic classification we applied a supervised machine learning algorithm, support vector machines (SVM). It requires a training sample with relevant classes already identified, and we chose to use the SDSS spectroscopic dataset (DR10) for that purpose. We tested the performance of two kernels used by the classifier, and determined the minimum number of sources in the training set required to achieve stable classification, as well as the minimum dimension of the parameter space. We also tested SVM classification accuracy as a function of extinction and apparent magnitude. Thus, the calibrated classifier was finally applied to all-sky WISE data, flux-limited to 16 mag (Vega) in the 3.4 μm channel. Results: By calibrating on the test data drawn from SDSS, we first established that a polynomial kernel is preferred over a radial one for this particular dataset. Next, using three classification parameters (W1 magnitude, W1-W2 colour, and a differential aperture magnitude) we obtained very good classification efficiency in all the tests. At the bright end, the completeness for stars and galaxies reaches ~95%, deteriorating to ~80% at W1 = 16 mag, while for quasars it stays at a level of

  2. Evaluation of tetrazolium-based semiautomatic colorimetric assay for measurement of human antitumor cytotoxicity

    Energy Technology Data Exchange (ETDEWEB)

    Heo, D.S.; Park, J.G.; Hata, K.; Day, R.; Herberman, R.B.; Whiteside, T.L. (Univ. of Pittsburgh School of Medicine, PA (USA))


    A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-based colorimetric assay was developed and compared with 51Cr release from different adherent tumor cell targets (human squamous cell carcinoma lines of the head and neck established in our laboratory, melanoma, and colorectal carcinoma) using 5-7-day human lymphokine-activated killer cells and monocyte-depleted peripheral blood lymphocytes as effectors. With adherent tumor cell targets, MTT colorimetry was more sensitive than the 51Cr release assay in measuring the antitumor activity of effectors: median, 4385 (range, 988-8144) versus median, 1061 (range, 582-7294) lytic units (the number of effector cells required to lyse 20% of 5 x 10(3) targets)/10(7) effectors (P less than 0.01). Background effects (without effector cells) were comparable in 4-h assays (9% versus 10%) between MTT colorimetry and 51Cr release. In 24-h assays, MTT colorimetry showed higher antitumor activity (70-100% versus 40-60% lysis at 1:1 effector:target cell ratio) but lower background effects (6% versus 38%) than 51Cr release assay. Thus, MTT colorimetry was more sensitive, did not use radiolabeled targets, required fewer effector cells, and was easier, less expensive, and better adaptable to serial monitoring of effector cell function in cancer patients. This colorimetric assay is especially well suited to adherent tumor cell targets. The use of adherent tumor cell monolayers, as opposed to trypsinized single cell suspensions, provides an opportunity to measure interactions of effector cells with enzymatically unaltered solid tumor targets. Because of the greater sensitivity of the colorimetric assay, the transformation of MTT data into lytic units, as commonly used for 51Cr release assays, required an adjustment to avoid the extrapolation based on the exponential fit equation.

  3. Cobalt source calibration

    Energy Technology Data Exchange (ETDEWEB)

    Rizvi, H.M.


    The data obtained from these tests determine the dose rate of the two cobalt sources in SRTC. Building 774-A houses one of these sources while the other resides in room C-067 of Building 773-A. The data from this experiment shows the following: (1) The dose rate of the No.2 cobalt source in Building 774-A measured 1.073 x 10{sup 5} rad/h (June 17, 1999). The dose rate of the Shepherd Model 109 Gamma cobalt source in Building 773-A measured 9.27 x 10{sup 5} rad/h (June 25, 1999). These rates come from placing the graduated cylinder containing the dosimeter solution in the center of the irradiation chamber. (2) Two calibration tests in the 774-A source placed the graduated cylinder with the dosimeter solution approximately 1.5 inches off center in the axial direction. This movement of the sample reduced the measured dose rate 0.92% from 1.083 x 10{sup 5} rad/h to 1.073 x 10{sup 5} rad/h. and (3) A similar test in the cobalt source in 773-A placed the graduated cylinder approximately 2.0 inches off center in the axial direction. This change in position reduced the measured dose rate by 10.34% from 1.036 x 10{sup 6} to 9.27 x 10{sup 5}. This testing used chemical dosimetry to measure the dose rate of a radioactive source. In this method, one determines the dose by the chemical change that takes place in the dosimeter. For this calibration experiment, the author used a Fricke (ferrous ammonium sulfate) dosimeter. This solution works well for dose rates to 10{sup 7} rad/h. During irradiation of the Fricke dosimeter solution the Fe{sup 2+} ions ionize to Fe{sup 3+}. When this occurs, the solution acquires a slightly darker tint (not visible to the human eye). To determine the magnitude of the change in Fe ions, one places the solution in an UV-VIS Spectrophotometer. The UV-VIS Spectrophotometer measures the absorbency of the solution. Dividing the absorbency by the total time (in minutes) of exposure yields the dose rate.

  4. The Advanced LIGO Photon Calibrators

    CERN Document Server

    Karki, S; Kandhasamy, S; Abbott, B P; Abbott, T D; Anders, E H; Berliner, J; Betzwieser, J; Daveloza, H P; Cahillane, C; Canete, L; Conley, C; Gleason, J R; Goetz, E; Kissel, J S; Izumi, K; Mendell, G; Quetschke, V; Rodruck, M; Sachdev, S; Sadecki, T; Schwinberg, P B; Sottile, A; Wade, M; Weinstein, A J; West, M; Savage, R L


    The two interferometers of the Laser Interferometry Gravitaional-wave Observatory (LIGO) recently detected gravitational waves from the mergers of binary black hole systems. Accurate calibration of the output of these detectors was crucial for the observation of these events, and the extraction of parameters of the sources. The principal tools used to calibrate the responses of the second-generation (Advanced) LIGO detectors to gravitational waves are systems based on radiation pressure and referred to as Photon Calibrators. These systems, which were completely redesigned for Advanced LIGO, include several significant upgrades that enable them to meet the calibration requirements of second-generation gravitational wave detectors in the new era of gravitational-wave astronomy. We report on the design, implementation, and operation of these Advanced LIGO Photon Calibrators that are currently providing fiducial displacements on the order of $10^{-18}$ m/$\\sqrt{\\textrm{Hz}}$ with accuracy and precision of better ...

  5. The Advanced LIGO photon calibrators (United States)

    Karki, S.; Tuyenbayev, D.; Kandhasamy, S.; Abbott, B. P.; Abbott, T. D.; Anders, E. H.; Berliner, J.; Betzwieser, J.; Cahillane, C.; Canete, L.; Conley, C.; Daveloza, H. P.; De Lillo, N.; Gleason, J. R.; Goetz, E.; Izumi, K.; Kissel, J. S.; Mendell, G.; Quetschke, V.; Rodruck, M.; Sachdev, S.; Sadecki, T.; Schwinberg, P. B.; Sottile, A.; Wade, M.; Weinstein, A. J.; West, M.; Savage, R. L.


    The two interferometers of the Laser Interferometry Gravitational-wave Observatory (LIGO) recently detected gravitational waves from the mergers of binary black hole systems. Accurate calibration of the output of these detectors was crucial for the observation of these events and the extraction of parameters of the sources. The principal tools used to calibrate the responses of the second-generation (Advanced) LIGO detectors to gravitational waves are systems based on radiation pressure and referred to as photon calibrators. These systems, which were completely redesigned for Advanced LIGO, include several significant upgrades that enable them to meet the calibration requirements of second-generation gravitational wave detectors in the new era of gravitational-wave astronomy. We report on the design, implementation, and operation of these Advanced LIGO photon calibrators that are currently providing fiducial displacements on the order of 1 0-18m /√{Hz } with accuracy and precision of better than 1%.


    Smith, V.L.; Carstensen, H.K.


    An improved time calibrated sweep circuit is presented, which extends the range of usefulness of conventional oscilloscopes as utilized for time calibrated display applications in accordance with U. S. Patent No. 2,832,002. Principal novelty resides in the provision of a pair of separate signal paths, each of which is phase and amplitude adjustable, to connect a high-frequency calibration oscillator to the output of a sawtooth generator also connected to the respective horizontal deflection plates of an oscilloscope cathode ray tube. The amplitude and phase of the calibration oscillator signals in the two signal paths are adjusted to balance out feedthrough currents capacitively coupled at high frequencies of the calibration oscillator from each horizontal deflection plate to the vertical plates of the cathode ray tube.

  7. Automated calibration of multistatic arrays

    Energy Technology Data Exchange (ETDEWEB)

    Henderer, Bruce


    A method is disclosed for calibrating a multistatic array having a plurality of transmitter and receiver pairs spaced from one another along a predetermined path and relative to a plurality of bin locations, and further being spaced at a fixed distance from a stationary calibration implement. A clock reference pulse may be generated, and each of the transmitters and receivers of each said transmitter/receiver pair turned on at a monotonically increasing time delay interval relative to the clock reference pulse. Ones of the transmitters and receivers may be used such that a previously calibrated transmitter or receiver of a given one of the transmitter/receiver pairs is paired with a subsequently un-calibrated one of the transmitters or receivers of an immediately subsequently positioned transmitter/receiver pair, to calibrate the transmitter or receiver of the immediately subsequent transmitter/receiver pair.

  8. Antenna Calibration and Measurement Equipment (United States)

    Rochblatt, David J.; Cortes, Manuel Vazquez


    A document describes the Antenna Calibration & Measurement Equipment (ACME) system that will provide the Deep Space Network (DSN) with instrumentation enabling a trained RF engineer at each complex to perform antenna calibration measurements and to generate antenna calibration data. This data includes continuous-scan auto-bore-based data acquisition with all-sky data gathering in support of 4th order pointing model generation requirements. Other data includes antenna subreflector focus, system noise temperature and tipping curves, antenna efficiency, reports system linearity, and instrument calibration. The ACME system design is based on the on-the-fly (OTF) mapping technique and architecture. ACME has contributed to the improved RF performance of the DSN by approximately a factor of two. It improved the pointing performances of the DSN antennas and productivity of its personnel and calibration engineers.

  9. Synthesis and evaluation of a new colorimetric and ratiometric fluorescence probe for copper ions

    Energy Technology Data Exchange (ETDEWEB)

    Chawla, Har Mohindra, E-mail:; Munjal, Priyanka; Goel, Preeti


    Synthesis and spectroscopic evaluation of compounds 3a, 3b and 4 reveal that cone conformer of 25,27-bis(o-aminothiophenyl propyloxy) -tetra-p-tert-butylcalix[4]arene 3a can function as a highly selective ratiometric and colorimetric fluorescence probe for copper ions. - Highlights: • We have synthesized a new calixarene based receptor 3 for Cu{sup 2+}. • 3 Showed ratiometric changes with Cu{sup 2+} in emission spectrum. • Reference compound 4 showed quenching with Cu{sup 2+} in emission spectrum. • Importance of calix[4]arene platform in ion recognition.

  10. DDTC-Na-based colorimetric chemosensor for the sensing of cyanide in water

    Institute of Scientific and Technical Information of China (English)


    Sodium diethyldithiocarbamate (DDTC-Na) was demonstrated to be a new colorimetric cyanide chemosensor by utilizing an indirect trick. First, some copper ions were added to the colorless aque- ous solution of DDTC-Na. Then, the resultant brown solution was studied upon the addition of different anions, including Cl-, I-, IO3-, SO42-, NO-2, Br-, H2PO4-, F-, SCN-, HSO-4, ClO-4 and CN-. It was observed by naked eyes that the brown solution changed to colorless immediately after the addition of the trace cyanide, but there were no changes towards other anions, making DDTC-Na a good selective cyanide chemosensor in pure water.

  11. Colorimetric and microscopic study of the thermal behavior of new ceramic pigments. (United States)

    Saviuc-Paval, Ana Mihaela; Victor Sandu, Andrei; Marcel Popa, Ionel; Anca Sandu, Irina Crina; Petru Bertea, Andrei; Sandu, Ion


    The article studies thermal resistance variation by analyzing the colorimetric parameters correlated with the optical microscopy data of two groups of ceramic pigments obtained by co-precipitation in aqueous medium of phosphate anion and of a mixture of chromium phosphate with various chromophore cations. This research enabled us to reveal the thermal thresholds/domains within which significant color changes occur, thus allowing the choice of pigments compatible with the thermal varnishing-glazing processes involved in the manufacture of tesserae for mosaic and stained glass and of colored materials for floor tiles, wall tiles and painted porcelain.

  12. Novel N,N'-Diacylhydrazine-Based Colorimetric Receptors for Selective Sensing of Fluoride and Acetate Anions

    Institute of Scientific and Technical Information of China (English)

    SHI, Da-Qing; WANG, Hai-Ying; LI, Xiao-Yue; YANG, Fang; SHI, Jing-Wen; WANG, Xiang-Shan


    Three novel and simple N,N'-diacylhydrazine-based colorimetric receptors have been prepared. The binding properties of the receptors to anions such as F-, Cl-, Br-, AcO-, HSO-4 and H2PO-4 in acetonitrile solution were examined by UV-Vis spectroscopy methods, which show high sensitivity and selectivity to F- and AcO- over other anions. The results indicated that a 1:1 stoichiometry complex was formed between the receptors and the anions, while 1H NMR titrations confirmed hydrogen binding interaction between the receptors and the anions.

  13. 2-Pyrrole Carboxylic Acid Nitro-Phenylamide: New Colorimetric Sensor for Anion

    Institute of Scientific and Technical Information of China (English)

    YIN Zhen-Ming; YANG Wen-Zhi; HE Jia-Qi; ZHU Xiao-Qing; CHENG Jin-Pei


    @@ Due to the role played by anions in the field of biology and environmental chemistry, the development of selec tive and sensitive chemosensor for anion sensing is a topic of current attention. Colorimetric anion sensor, which does not require the use of a potentiostate or spectrometer to detect redox or optical perturbation, can give immediate qualitative anion sensing information by visual detection and therefore has advantages over other molecular sensors.According the anion binding ability of some pyrrolic amides reported by Schmuck and Gale, we linked the color reporter group of nitroanile to pyrrole moiety and synthesized two 2-pyrrole carboxylic acid nitro-phenylamides (1 and 2).

  14. Polycaprolactone-Polydiacetylene Electrospun Fibers for Colorimetric Detection of Fake Gasoline

    Directory of Open Access Journals (Sweden)

    Shamshad Ali


    Full Text Available PCDA (Pentacosadiynoic Acid monomers were successfully embedded in PCL (Poly ?-Caprolactone polymer matrix by electrospinning process for the first time. The resultant EFM (Electrospun Fibers Mat was photo-polymerized under 254 nm UV light that enables colorimetric detection of fake gasoline. Results revealed that the fake gasoline develops a red color mat within 5 sec. FE-SEM images showed that the fake gasoline treatment dissolved the PCL EFM that give access to interact with PDA polymer. The proposed litmus-type sensor based on PCL-PDA EFM is highly sensitive to fake gasoline and can be fabricated easily

  15. Colorimetric and fluorescence detection of G-quadruplex nucleic acids with a coumarin-benzothiazole probe. (United States)

    Yan, Jin-wu; Tian, Yi-guang; Tan, Jia-heng; Huang, Zhi-shu


    A colorimetric and red-emitting fluorescent dual probe for G-quadruplexes was devised with a conjugated coumarin-benzothiazole scaffold. Its significant and distinct changes in both color and fluorescence enable the label-free and visual detection of G-quadruplex structures. In addition, this probe gives a distinct strong emission response to the nucleoli in fixed cells imaging, which might be attributed to the interaction between the probe and rDNA G-quadruplex based on the chromatin immunoprecipitation assay. All these results suggest its promising application prospects in the G-quadruplex research field.

  16. A gold nanoparticles-based colorimetric test to detect single nucleotide polymorphisms for improvement of personalized therapy of psoriasis (United States)

    Marsella, Alessandra; Valentini, Paola; Tarantino, Paolo; Congedo, Maurizio; Pompa, Pier Paolo


    We report a simple, rapid and low-cost test, based on gold nanoparticles, for the naked-eye colorimetric detection of a signature of single nucleotide polymorphisms (SNPs) relevant for the personalized medicine of psoriasis patients. We validated the colorimetric assay on real-world DNA samples from a cohort of 30 psoriasis patients and we compared the results, in double-blind, with those obtained with two state-of-the-art instrumental techniques, namely reverse dot blotting and direct sequencing, finding 100% agreement. We demonstrated high accuracy, sensitivity and specificity of the colorimetric test that can be easily adapted for the genotypization of different SNPs, important for the pharmacogenomics of various diseases, and in other fields, such as food traceability and population structure analysis.

  17. Colorimetric sensor array allows fast detection and simultaneous identification of sepsis-causing bacteria in spiked blood culture. (United States)

    Lim, Sung H; Mix, Samantha; Xu, Zeyu; Taba, Brian; Budvytiene, Indre; Berliner, Anders N; Queralto, Nuria; Churi, Yair S; Huang, Richard S; Eiden, Michael; Martino, Raymond A; Rhodes, Paul; Banaei, Niaz


    Sepsis is a medical emergency demanding early diagnosis and tailored antimicrobial therapy. Every hour of delay in initiating effective therapy measurably increases patient mortality. Blood culture is currently the reference standard for detecting bloodstream infection, a multistep process which may take one to several days. Here, we report a novel paradigm for earlier detection and the simultaneous identification of pathogens in spiked blood cultures by means of a metabolomic "fingerprint" of the volatile mixture outgassed by the organisms. The colorimetric sensor array provided significantly faster detection of positive blood cultures than a conventional blood culture system (12.1 h versus 14.9 h, P detection. The colorimetric sensor array also allowed for discrimination between unrelated strains of methicillin-resistant Staphylococcus aureus, indicating that the metabolomic fingerprint has the potential to track nosocomial transmissions. Altogether, the colorimetric sensor array is a promising tool that offers a new paradigm for diagnosing bloodstream infections.

  18. A colorimetric method for highly sensitive and accurate detection of iodide by finding the critical color in a color change process using silver triangular nanoplates

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Xiu-Hua; Ling, Jian, E-mail:; Peng, Jun; Cao, Qiu-E., E-mail:; Ding, Zhong-Tao; Bian, Long-Chun


    Graphical abstract: -- Highlights: •Demonstrated a new colorimetric strategy for iodide detection by silver nanoplates. •The colorimetric strategy is to find the critical color in a color change process. •The colorimetric strategy is more accurate and sensitive than common colorimetry. •Discovered a new morphological transformation phenomenon of silver nanoplates. -- Abstract: In this contribution, we demonstrated a novel colorimetric method for highly sensitive and accurate detection of iodide using citrate-stabilized silver triangular nanoplates (silver TNPs). Very lower concentration of iodide can induce an appreciable color change of silver TNPs solution from blue to yellow by fusing of silver TNPs to nanoparticles, as confirmed by UV–vis absorption spectroscopy and transmission electron microscopy (TEM). The principle of this colorimetric assay is not an ordinary colorimetry, but a new colorimetric strategy by finding the critical color in a color change process. With this strategy, 0.1 μM of iodide can be recognized within 30 min by naked-eyes observation, and lower concentration of iodide down to 8.8 nM can be detected using a spectrophotometer. Furthermore, this high sensitive colorimetric assay has good accuracy, stability and reproducibility comparing with other ordinary colorimetry. We believe this new colorimetric method will open up a fresh insight of simple, rapid and reliable detection of iodide and can find its future application in the biochemical analysis or clinical diagnosis.

  19. A calibrated Franklin chimes (United States)

    Gonta, Igor; Williams, Earle


    Benjamin Franklin devised a simple yet intriguing device to measure electrification in the atmosphere during conditions of foul weather. He constructed a system of bells, one of which was attached to a conductor that was suspended vertically above his house. The device is illustrated in a well-known painting of Franklin (Cohen, 1985). The elevated conductor acquired a potential due to the electric field in the atmosphere and caused a brass ball to oscillate between two bells. The purpose of this study is to extend Franklin's idea by constructing a set of 'chimes' which will operate both in fair and in foul weather conditions. In addition, a mathematical relationship will be established between the frequency of oscillation of a metallic sphere in a simplified geometry and the potential on one plate due to the electrification of the atmosphere. Thus it will be possible to calibrate the 'Franklin Chimes' and to obtain a nearly instantaneous measurement of the potential of the elevated conductor in both fair and foul weather conditions.

  20. A Visual Servoing-Based Method for ProCam Systems Calibration

    Directory of Open Access Journals (Sweden)

    Jeremie Mosnier


    Full Text Available Projector-camera systems are currently used in a wide field of applications, such as 3D reconstruction and augmented reality, and can provide accurate measurements, depending on the configuration and calibration. Frequently, the calibration task is divided into two steps: camera calibration followed by projector calibration. The latter still poses certain problems that are not easy to solve, such as the difficulty in obtaining a set of 2D–3D points to compute the projection matrix between the projector and the world. Existing methods are either not sufficiently accurate or not flexible. We propose an easy and automatic method to calibrate such systems that consists in projecting a calibration pattern and superimposing it automatically on a known printed pattern. The projected pattern is provided by a virtual camera observing a virtual pattern in an OpenGL model. The projector displays what the virtual camera visualizes. Thus, the projected pattern can be controlled and superimposed on the printed one with the aid of visual servoing. Our experimental results compare favorably with those of other methods considering both usability and accuracy.

  1. Self-calibration algorithm in an asynchronous P300-based brain-computer interface (United States)

    Schettini, F.; Aloise, F.; Aricò, P.; Salinari, S.; Mattia, D.; Cincotti, F.


    Objective. Reliability is a desirable characteristic of brain-computer interface (BCI) systems when they are intended to be used under non-experimental operating conditions. In addition, their overall usability is influenced by the complex and frequent procedures that are required for configuration and calibration. Earlier studies examined the issue of asynchronous control in P300-based BCIs, introducing dynamic stopping and automatic control suspension features. This report proposes and evaluates an algorithm for the automatic recalibration of the classifier's parameters using unsupervised data. Approach. Ten healthy subjects participated in five P300-based BCI sessions throughout a single day. First, we examined whether continuous adaptation of control parameters improved the accuracy of the asynchronous system over time. Then, we assessed the performance of the self-calibration algorithm with respect to the no-recalibration and supervised calibration conditions with regard to system accuracy and communication efficiency. Main results. Offline tests demonstrated that continuous adaptation of the control parameters significantly increased the communication efficiency of asynchronous P300-based BCIs. The self-calibration algorithm correctly assigned labels to unsupervised data with 95% accuracy, effecting communication efficiency that was comparable with that of supervised repeated calibration. Significance. Although additional online tests that involve end-users under non-experimental conditions are needed, these preliminary results are encouraging, from which we conclude that the self-calibration algorithm is a promising solution to improve P300-based BCI usability and reliability.

  2. A visual servoing-based method for ProCam systems calibration. (United States)

    Berry, Francois; Aider, Omar Ait; Mosnier, Jeremie


    Projector-camera systems are currently used in a wide field of applications, such as 3D reconstruction and augmented reality, and can provide accurate measurements, depending on the configuration and calibration. Frequently, the calibration task is divided into two steps: camera calibration followed by projector calibration. The latter still poses certain problems that are not easy to solve, such as the difficulty in obtaining a set of 2D-3D points to compute the projection matrix between the projector and the world. Existing methods are either not sufficiently accurate or not flexible. We propose an easy and automatic method to calibrate such systems that consists in projecting a calibration pattern and superimposing it automatically on a known printed pattern. The projected pattern is provided by a virtual camera observing a virtual pattern in an OpenGL model. The projector displays what the virtual camera visualizes. Thus, the projected pattern can be controlled and superimposed on the printed one with the aid of visual servoing. Our experimental results compare favorably with those of other methods considering both usability and accuracy.


    Energy Technology Data Exchange (ETDEWEB)

    Jon P. Christophersen; John L. Morrison; William H. Morrison


    Electrochemical impedance spectroscopy has been shown to be a valuable tool for diagnostics and prognostics of energy storage devices such as batteries and ultra-capacitors. Although measurements have been typically confined to laboratory environments, rapid impedance spectrum measurement techniques have been developed for on-line, embedded applications as well. The prototype hardware for the rapid technique has been validated using lithium-ion batteries, but issues with calibration had also been identified. A new, universal automatic calibration technique was developed to address the identified issues while also enabling a more simplified approach. A single, broad-frequency range is used to calibrate the system and then scaled to the actual range and conditions used when measuring a device under test. The range used for calibration must be broad relative to the expected measurement conditions for the scaling to be successful. Validation studies were performed by comparing the universal calibration approach with data acquired from targeted calibration ranges based on the expected range of performance for the device under test. First, a mid-level shunt range was used for calibration and used to measure devices with lower and higher impedance. Next, a high excitation current level was used for calibration, followed by measurements using lower currents. Finally, calibration was performed over a wide frequency range and used to measure test articles with a lower set of frequencies. In all cases, the universal calibration approach compared very well with results acquired following a targeted calibration. Additionally, the shunts used for the automated calibration technique were successfully characterized such that the rapid impedance measurements compare very well with laboratory-scale measurements. These data indicate that the universal approach can be successfully used for onboard rapid impedance spectra measurements for a broad set of test devices and range of

  4. Automatic crystal lookup table generation for PET detector calibration by fusion of information from multiple approaches%基于多种探测算法结果分级融合的PET探测器晶体查找表建立算法

    Institute of Scientific and Technical Information of China (English)

    李丽平; 周鑫; 李强


    高分辨率是现代正电子发射型断层显像仪(Positron Emission Tomography,PET)设备最重要的技术指标之一,高分辨率PET探测器通常由海量闪烁晶体组成,这使得探测器校准时,晶体查找表建立的工作量大幅增加,从而对相关自动化算法的鲁棒性提出了更高的要求.目前,晶体阵列的矫正主要依赖固定放射源对探测器晶体阵列成像得到的光子二维位置直方图.高分辨率PET探测器的晶体阵列中晶体个数增多,尺寸变小,导致二维位置直方图信噪比下降,且非线性形变更加复杂,使已有的晶体查找表建立算法都无法得到理想的自动化效率.在测试了多种前沿的晶体查找表建立算法后发现,某些算法的结果之间有很强的互补性,可以通过将多种算法结果相融合的方法得到优于单一探测算法的结果.因此提出了一种基于多种探测算法结果分级融合的晶体查找表建立算法,在实现过程中选取了三种互补性较强的晶体探测方法,分级融合其结果,大幅度降低了二维位置直方图上晶体分割的出错概率,获得了鲁棒的结果.%Background: High resolution is of key importance for positron emission tomography (PET) devices. Modern PET scanners use a large number of crystals in detector blocks to improve their resolution. The extra crystals lead to a significant increase in workload of crystal identification in PET detector calibration. Hence the robustness of crystal identification algorithms becomes a challenge. Crystal identification is typically based on flood histograms obtained by irradiating the detector blocks with an annihilation photonflood source. More crystals of smaller size degrade the quality of flood histogram and generate more complex spatial distortions. Existing crystal identification methods are not robust enough to handle these cases.Purpose: Our study aims to improve the robustness and success rate of crystal identification on PET

  5. Mercury Continuous Emmission Monitor Calibration

    Energy Technology Data Exchange (ETDEWEB)

    John Schabron; Eric Kalberer; Ryan Boysen; William Schuster; Joseph Rovani


    Mercury continuous emissions monitoring systems (CEMs) are being implemented in over 800 coal-fired power plant stacks throughput the U.S. Western Research Institute (WRI) is working closely with the Electric Power Research Institute (EPRI), the National Institute of Standards and Technology (NIST), and the Environmental Protection Agency (EPA) to facilitate the development of the experimental criteria for a NIST traceability protocol for dynamic elemental mercury vapor calibrators/generators. These devices are used to calibrate mercury CEMs at power plant sites. The Clean Air Mercury Rule (CAMR) which was published in the Federal Register on May 18, 2005 and vacated by a Federal appeals court in early 2008 required that calibration be performed with NIST-traceable standards. Despite the vacature, mercury emissions regulations in the future will require NIST traceable calibration standards, and EPA does not want to interrupt the effort towards developing NIST traceability protocols. The traceability procedures will be defined by EPA. An initial draft traceability protocol was issued by EPA in May 2007 for comment. In August 2007, EPA issued a conceptual interim traceability protocol for elemental mercury calibrators. The protocol is based on the actual analysis of the output of each calibration unit at several concentration levels ranging initially from about 2-40 {micro}g/m{sup 3} elemental mercury, and in the future down to 0.2 {micro}g/m{sup 3}, and this analysis will be directly traceable to analyses by NIST. The EPA traceability protocol document is divided into two separate sections. The first deals with the qualification of calibrator models by the vendors for use in mercury CEM calibration. The second describes the procedure that the vendors must use to certify the calibrators that meet the qualification specifications. The NIST traceable certification is performance based, traceable to analysis using isotope dilution inductively coupled plasma

  6. Spatial 3-D nonlinear calibration technique for PSD (United States)

    Guo, Lifeng; Zhang, Guoxiong; Zheng, Qi; Gong, Qiang; Liu, Wenyao


    A 3-D nonlinear calibration technique for Position sensitive detector (PSD) in long distance laser collimating measurement is proposed. An automatic calibration system was developed to measure the nonlinearity of a 2-D PSD in 3-D space. It is mainly composed of a high accurate 2-D motorized translational stage, a high precision distance measuring device, and a computer-based data acquisition and control system. With the aid of the calibration system, the nonlinear characteristic of 2-D PSD is checked in a long collimating distance up to 78 meters. The calibration experiment was carried out for a series of distance, e.g. every 15 meters. The results showed that the nonlinearity of 2-D PSD is different evidently when the PSD element is at different distance from the laser head. One calculating method is defined to evaluate the nonlinear errors. The spatial 3-D mapping relationship between the actual displacements of the incident light and the coordinates of 2-D PSD outputs is established using a multilayer feedforward neural network.

  7. Spring gravimeter calibration experiment with an inertial acceleration platform

    CERN Document Server

    Zhu, Ping; Noel, Jean-Phillippe


    This experiment tested an automatically calibrate the relatively gravimeter with an absolute inertial force. The whole calibration system was controlled by microprocessor and low frequency oscillations were generated by a step motor. It could produce different period low frequency sinusoid inertial force. A LVDT (Linear Variable Differential Transformer) sensor was introduced to determine the frequency transfer function and the stability of platform vertical motion. The error of vertical displacement induced by the mechanical part of platform is less than 0.01 mm. Two LCR gravimeters (G336, G906) were settled on the platform. G336 was in the centre of platform and G906 was in the left side. A calibration program has been integrated in the micro processor which could send calibrate signal once a week. During the more than 7 moths experiment, the scale factor of G336 is 8128.647 nm/s2/v with 1.1% uncertainties and G906 is 9421.017 nm/s2/v with 2.7% fluctuations.

  8. Peristaltic pump-based low range pressure sensor calibration system. (United States)

    Vinayakumar, K B; Naveen Kumar, G; Nayak, M M; Dinesh, N S; Rajanna, K


    Peristaltic pumps were normally used to pump liquids in several chemical and biological applications. In the present study, a peristaltic pump was used to pressurize the chamber (positive as well negative pressures) using atmospheric air. In the present paper, we discuss the development and performance study of an automatic pressurization system to calibrate low range (millibar) pressure sensors. The system includes a peristaltic pump, calibrated pressure sensor (master sensor), pressure chamber, and the control electronics. An in-house developed peristaltic pump was used to pressurize the chamber. A closed loop control system has been developed to detect and adjust the pressure leaks in the chamber. The complete system has been integrated into a portable product. The system performance has been studied for a step response and steady state errors. The system is portable, free from oil contaminants, and consumes less power compared to existing pressure calibration systems. The veracity of the system was verified by calibrating an unknown diaphragm based pressure sensor and the results obtained were satisfactory.

  9. Mexican national pyronometer network calibration (United States)

    VAldes, M.; Villarreal, L.; Estevez, H.; Riveros, D.


    In order to take advantage of the solar radiation as an alternate energy source it is necessary to evaluate the spatial and temporal availability. The Mexican National Meterological Service (SMN) has a network with 136 meteorological stations, each coupled with a pyronometer for measuring the global solar radiation. Some of these stations had not been calibrated in several years. The Mexican Department of Energy (SENER) in order to count on a reliable evaluation of the solar resource funded this project to calibrate the SMN pyrometer network and validate the data. The calibration of the 136 pyronometers by the intercomparison method recommended by the World Meterological Organization (WMO) requires lengthy observations and specific environmental conditions such as clear skies and a stable atmosphere, circumstances that determine the site and season of the calibration. The Solar Radiation Section of the Instituto de Geofísica of the Universidad Nacional Autónoma de México is a Regional Center of the WMO and is certified to carry out the calibration procedures and emit certificates. We are responsible for the recalibration of the pyronometer network of the SMN. A continuous emission solar simulator with exposed areas with 30cm diameters was acquired to reduce the calibration time and not depend on atmospheric conditions. We present the results of the calibration of 10 thermopile pyronometers and one photovoltaic cell by the intercomparison method with more than 10000 observations each and those obtained with the solar simulator.

  10. Jet energy calibration in ATLAS

    CERN Document Server

    Schouten, Doug

    A correct energy calibration for jets is essential to the success of the ATLAS experi- ment. In this thesis I study a method for deriving an in situ jet energy calibration for the ATLAS detector. In particular, I show the applicability of the missing transverse energy projection fraction method. This method is shown to set the correct mean energy for jets. Pileup effects due to the high luminosities at ATLAS are also stud- ied. I study the correlations in lateral distributions of pileup energy, as well as the luminosity dependence of the in situ calibration metho

  11. Calibrating System for Vacuum Gauges

    Institute of Scientific and Technical Information of China (English)

    MengJun; YangXiaotian; HaoBinggan; HouShengjun; HuZhenjun


    In order to measure the vacuum degree, a lot of vacuum gauges will be used in CSR vacuum system. We bought several types of vacuum gauges. We know that different typos of vacuum gauges or even one type of vacuum gauges have different measure results in same condition, so they must be calibrated. But it seems impossible for us to send so many gauges to the calibrating station outside because of the high price. So the best choice is to build a second class calibrating station for vacuum gauges by ourselves (Fig.l).

  12. A selective colorimetric and ratiometric fluorescent chemosensor for detection of Al{sup 3+} ion

    Energy Technology Data Exchange (ETDEWEB)

    Hung, Pei-Ju; Chir, Jiun-Ly; Ting, Wei; Wu, An-Tai, E-mail:


    A simple Schiff-base fluorescent receptor 1 was synthesized in an one step procedure. Receptor 1 displayed a selective colorimetric change (from light yellow to light green) and high ratiometric signals upon binding to Al{sup 3+} in EtOH solution. The association constant for 1–Al{sup 3+} in EtOH was determined as 2.08×10{sup 6} M{sup −2} and the detection limit for Al{sup 3+} was determined as 5.21×10{sup −7} M. The detection limit was sufficiently low to detect submicromolar concentration of the Al{sup 3+}. - Highlights: • A new Al{sup 3+} receptor can be easily prepared. • The receptor exhibits an “off-on-type” mode with high selectivity in the presence of Al{sup 3+}. • Receptor 1 could serve as selective and efficient colorimetric sensor for the detection of Al{sup 3+} by naked eye method.

  13. CUPRAC colorimetric and electroanalytical methods determining antioxidant activity based on prevention of oxidative DNA damage. (United States)

    Uzunboy, Seda; Çekiç, Sema Demirci; Eksin, Ece; Erdem, Arzum; Apak, Reşat


    An unbalanced excess of oxygen/nitrogen species (ROS/RNS) can give oxidative hazard to DNA and other biomacromolecules under oxidative stress conditions. While the 'comet' assay for measuring DNA damage is neither specific nor practical, monitoring oxidative changes on individual DNA bases and other oxidation products needs highly specialized equipment and operators. Thus, we developed a modified CUPRAC (cupric ion reducing antioxidant capacity) colorimetric method to determine the average total damage on DNA produced by Fenton oxidation, taking advantage of the fact that the degradation products of DNA but not the original macromolecule is CUPRAC-responsive. The DNA-protective effects of water-soluble antioxidants were used to devise a novel antioxidant activity assay, considered to be physiologically more realistic than those using artificial probes. Our method, based on the measurement of DNA oxidative products with CUPRAC colorimetry proved to be 2 orders-of-magnitude more sensitive than the widely used TBARS (thiobarbituric acid-reactive substances) colorimetric assay used as reference. Additionally, the DNA damage was electrochemically investigated using pencil graphite electrodes (PGEs) as DNA sensor platform in combination with differential pulse voltammetry (DPV). The interaction of the radical species with DNA in the absence/presence of antioxidants was detected according to the changes in guanine oxidation signal.

  14. A High Affinity Red Fluorescence and Colorimetric Probe for Amyloid β Aggregates (United States)

    Rajasekhar, K.; Narayanaswamy, Nagarjun; Murugan, N. Arul; Kuang, Guanglin; Ågren, Hans; Govindaraju, T.


    A major challenge in the Alzheimer’s disease (AD) is its timely diagnosis. Amyloid β (Aβ) aggregates have been proposed as the most viable biomarker for the diagnosis of AD. Here, we demonstrate hemicyanine-based benzothiazole-coumarin (TC) as a potential probe for the detection of highly toxic Aβ42 aggregates through switch-on, enhanced (~30 fold) red fluorescence (Emax = 654 nm) and characteristic colorimetric (light red to purple) optical outputs. Interestingly, TC exhibits selectivity towards Aβ42 fibrils compared to other abnormal protein aggregates. TC probe show nanomolar binding affinity (Ka = 1.72 × 107 M‑1) towards Aβ42 aggregates and also displace ThT bound to Aβ42 fibrils due to its high binding affinity. The Aβ42 fibril-specific red-shift in the absorption spectra of TC responsible for the observed colorimetric optical output has been attributed to micro-environment change around the probe from hydrophilic-like to hydrophobic-like nature. The binding site, binding energy and changes in optical properties observed for TC upon interaction with Aβ42 fibrils have been further validated by molecular docking and time dependent density functional theory studies.

  15. Simple colorimetric detection of doxycycline and oxytetracycline using unmodified gold nanoparticles (United States)

    Li, Jie; Fan, Shumin; Li, Zhigang; Xie, Yuanzhe; Wang, Rui; Ge, Baoyu; Wu, Jing; Wang, Ruiyong


    The interaction between tetracycline antibiotics and gold nanoparticles was studied. With citrate-coated gold nanoparticles as colorimetric probe, a simple and rapid detection method for doxycycline and oxytetracycline has been developed. This method relies on the distance-dependent optical properties of gold nanoparticles. In weakly acidic buffer medium, doxycycline and oxytetracycline could rapidly induce the aggregation of gold nanoparticles, resulting in red-to-blue (or purple) colour change. The experimental parameters were optimized with regard to pH, the concentration of the gold nanoparticles and the reaction time. Under optimal experimental conditions, the linear range of the colorimetric sensor for doxycycline/oxytetracycline was 0.06-0.66 and 0.59-8.85 μg mL-1, respectively. The corresponding limit of detection for doxycycline and oxytetracycline was 0.0086 and 0.0838 μg mL-1, respectively. This assay was sensitive, selective, simple and readily used to detect tetracycline antibiotics in food products.

  16. Colorimetric determination of nitrate plus nitrite in water by enzymatic reduction, automated discrete analyzer methods (United States)

    Patton, Charles J.; Kryskalla, Jennifer R.


    This report documents work at the U.S. Geological Survey (USGS) National Water Quality Laboratory (NWQL) to validate enzymatic reduction, colorimetric determinative methods for nitrate + nitrite in filtered water by automated discrete analysis. In these standard- and low-level methods (USGS I-2547-11 and I-2548-11), nitrate is reduced to nitrite with nontoxic, soluble nitrate reductase rather than toxic, granular, copperized cadmium used in the longstanding USGS automated continuous-flow analyzer methods I-2545-90 (NWQL laboratory code 1975) and I-2546-91 (NWQL laboratory code 1979). Colorimetric reagents used to determine resulting nitrite in aforementioned enzymatic- and cadmium-reduction methods are identical. The enzyme used in these discrete analyzer methods, designated AtNaR2 by its manufacturer, is produced by recombinant expression of the nitrate reductase gene from wall cress (Arabidopsis thaliana) in the yeast Pichia pastoris. Unlike other commercially available nitrate reductases we evaluated, AtNaR2 maintains high activity at 37°C and is not inhibited by high-phenolic-content humic acids at reaction temperatures in the range of 20°C to 37°C. These previously unrecognized AtNaR2 characteristics are essential for successful performance of discrete analyzer nitrate + nitrite assays (henceforth, DA-AtNaR2) described here.

  17. A colorimetric detection method of pesticide acetamiprid by fine-tuning aptamer length. (United States)

    Tian, Yu; Wang, Yuan; Sheng, Zhi; Li, Tingting; Li, Xu


    This work investigates the effect of shortening aptamer sequences on the colorimetric detection of acetamiprid using aptamer-wrapped gold nanoparticles (AuNPs). Truncated 37-mer and 25-mer aptamers were generated by deleting excess flanking nucleotides from parental 49-mer acetamiprid-target aptamer. In comparing the responses of the three sequences, truncated aptamers did not improve the ability to discriminate against other tested pesticides. However, comparison between 49-mer and other shorter aptamers showed that shortening aptamer sequences through removing excess flanking nucleotides outsides of binding region improved colorimetric sensitivity for acetamiprid by 3.3 fold. Due to excess bases, the target-bound aptamer might still adhere to AuNPs, resulting in incomplete dissociation of aptamer from AuNPs and therefore the suppression of aggregation responses. This work provides further insight to the effects of aptamer structure on detection of the target, as well as a method by fine-tuning aptamer length for rapid detection of pesticide residues in environments or food.

  18. Centrifugal loop-mediated isothermal amplification microdevice for rapid, multiplex and colorimetric foodborne pathogen detection. (United States)

    Oh, Seung Jun; Park, Byung Hyun; Jung, Jae Hwan; Choi, Goro; Lee, Doh C; Kim, Do Hyun; Seo, Tae Seok


    We present a centrifugal microfluidic device which enables multiplex foodborne pathogen identification by loop-mediated isothermal amplification (LAMP) and colorimetric detection using Eriochrome Black T (EBT). Five identical structures were designed in the centrifugal microfluidic system to perform the genetic analysis of 25 pathogen samples in a high-throughput manner. The sequential loading and aliquoting of the LAMP cocktail, the primer mixtures, and the DNA sample solutions were accomplished by the optimized zigzag-shaped microchannels and RPM control. We targeted three kinds of pathogenic bacteria (Escherichia coli O157:H7, Salmonella typhimurium and Vibrio parahaemolyticus) and detected the amplicons of the LAMP reaction by the EBT-mediated colorimetric method. For the limit-of-detection (LOD) test, we carried out the LAMP reaction on a chip with serially diluted DNA templates of E. coli O157:H7, and could observe the color change with 380 copies. The used primer sets in the LAMP reaction were specific only to the genomic DNA of E. coli O157:H7, enabling the on-chip selective, sensitive, and high-throughput pathogen identification with the naked eyes. The entire process was completed in 60min. Since the proposed microsystem does not require any bulky and expensive instrumentation for end-point detection, our microdevice would be adequate for point-of-care (POC) testing with high simplicity and high speed, providing an advanced genetic analysis microsystem for foodborne pathogen detection.

  19. Plasmonic Metasurfaces Based on Nanopin-Cavity Resonator for Quantitative Colorimetric Ricin Sensing. (United States)

    Fan, Jiao-Rong; Zhu, Jia; Wu, Wen-Gang; Huang, Yun


    In view of the toxic potential of a bioweapon threat, rapid visual recognition and sensing of ricin has been of considerable interest while remaining a challenging task up to date. In this study, a gold nanopin-based colorimetric sensor is developed realizing a multicolor variation for ricin qualitative recognition and analysis. It is revealed that such plasmonic metasurfaces based on nanopin-cavity resonator exhibit reflective color appearance, due to the excitation of standing-wave resonances of narrow bandwidth in visible region. This clear color variation is a consequence of the reflective color mixing defined by different resonant wavelengths. In addition, the colored metasurfaces appear sharp color difference in a narrow refractive index range, which makes them especially well-suited for sensing applications. Therefore, this antibody-functionalized nanopin-cavity biosensor features high sensitivity and fast response, allowing for visual quantitative ricin detection within the range of 10-120 ng mL(-1) (0.15 × 10(-9) -1.8 × 10(-9) m), a limit of detection of 10 ng mL(-1) , and the typical measurement time of less than 10 min. The on-chip integration of such nanopin metasurfaces to portable colorimetric microfluidic device may be envisaged for the quantitative studies of a variety of biochemical molecules.

  20. A New Coumarin-Based Colorimetric and Fluorometric Sensor for Cu{sup 2+}

    Energy Technology Data Exchange (ETDEWEB)

    An, Kyounglyong; Jun, Kun [Korea Research Institute of Chemical Technology, Daejeon (Korea, Republic of); Park, Koon Ha [Chungnam National Univ., Daejeon (Korea, Republic of)


    We have developed a new colorimetric and fluorescent 'turn-off' sensor for Cu{sup 2+} based on coumarin Shiff base of hydroxycinnamaldehyde. It displays a 50 nm red-shift of maximum absorption band with color change from colorless to greenish-yellow upon addition of Cu{sup 2+} in 10 mM tris-HCl buffer solution (acetonitrile/water = 9:1, pH = 7.01). And also remarkable fluorescence quenching was observed upon the addition of Cu{sup 2+}. The 1:2 stoichiometry of sensor complex (sensor A + Cu{sup 2+}) was confirmed by Job's plot based on absorption titration. Chemosensors, small chemical compounds that sense the presence of analytes or energy, typically consist of two components: a receptor moiety that interacts with the target analytes and a read-out system that signals binding. And one of the most utilized research tool for the study of chemosensors employs a colorimetric and fluorometric spectroscopic techniques. So far successful reports on metal ion sensors have been documented including our recent result. Many different kinds of optical or fluorescent sensors have several advantages (such as high sensitivity and selectivity, non-destructive analysis, low cost and real-time monitoring), which allow naked-eye detection of color and fluorescent emission change upon metal ion binding without the use of any expensive spectroscopic equipment.

  1. Colorimetric determination of sildenafil citrate (Viagra) through ion-associate complex formation. (United States)

    Amin, Alaa S; Moustafa, Moustafa E; El-Dosoky, Reham


    A simple, quick, accurate, and sensitive colorimetric method is described for the determination of sildenafil citrate (SLD). The method is based on the reaction of SLD with Congo Red, Sudan II, and Gentian Violet in buffered aqueous solutions at pH 2.5, 6.5, and 11.0, respectively, to give highly colored soluble ion-associate complex species; the colored products are quantitated colorimetrically at 523, 554, and 569 nm, respectively. The various experimental conditions were optimized. The stoichiometric ratio was found to be 1:1 for all ion associates; the calculated logarithmic stability constants were 8.51, 7.79, and 5.58, respectively. Beer's law was obeyed over the concentration range of 0.2-7.0 microg/mL, whereas the Ringbom optimum concentration range was 0.4-6.5 microg/mL. Values for molar absorptivity, Sandell sensitivity, and detection and quantification limits were also calculated. The proposed method was successfully applied to the determination of SLD in Viagra tablets and in serum samples by using the technique of standard additions with mean accuracy values of 100.06 +/- 1.14, 99.87 +/- 0.70, and 99.86 +/- 0.97% for Viagra tablets and 99.88 +/- 0.60, 99.90 +/- 0.90, and 100.24 +/- 0.80% for serum samples, respectively.

  2. Beetroot-pigment-derived colorimetric sensor for detection of calcium dipicolinate in bacterial spores.

    Directory of Open Access Journals (Sweden)

    Letícia Christina Pires Gonçalves

    Full Text Available In this proof-of-concept study, we describe the use of the main red beet pigment betanin for the quantification of calcium dipicolinate in bacterial spores, including Bacillus anthracis. In the presence of europium(III ions, betanin is converted to a water-soluble, non-luminescent orange 1∶1 complex with a stability constant of 1.4 × 10(5 L mol(-1. The addition of calcium dipicolinate, largely found in bacterial spores, changes the color of the aqueous solution of [Eu(Bn(+] from orange to magenta. The limit of detection (LOD of calcium dipicolinate is around 2.0 × 10(-6 mol L(-1 and the LOD determined for both spores, B. cereus and B. anthracis, is (1.1 ± 0.3× 10(6 spores mL(-1. This simple, green, fast and low cost colorimetric assay was selective for calcium dipicolinate when compared to several analogous compounds. The importance of this work relies on the potential use of betalains, raw natural pigments, as colorimetric sensors for biological applications.

  3. An effective colorimetric and ratiometric fluorescent probe for bisulfite in aqueous solution

    Energy Technology Data Exchange (ETDEWEB)

    Dai, Xi [Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100 (China); Zhang, Tao [Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100 (China); Du, Zhi-Fang; Cao, Xiang-Jian; Chen, Ming-Yu [Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100 (China); Taishan College, Shandong University, Jinan 250100 (China); Hu, Sheng-Wen [Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100 (China); Miao, Jun-Ying, E-mail: [Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100 (China); Zhao, Bao-Xiang, E-mail: [Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100 (China)


    We have developed the first two-photon colorimetric and ratiometric fluorescent probe, BICO, for the detection of bisulfite (HSO{sub 3}{sup −}) in aqueous solution. The probe contains coumarin and benzimidazole moieties and can detect HSO{sub 3}{sup −} based on the Michael addition reaction with a limit of detection 5.3 × 10{sup −8} M in phosphate-buffered saline solution. The probe was used to detect bisulfite in tap water, sugar and dry white wine. Moreover, test strips were made and used easily. We successfully applied the probe to image living cells, using one-photon fluorescence imaging. BICO overcomes the limitations in sensitivity of previously reported probes and the solvation effect of bisulfite, which demonstrates its excellent value in practical application. - Highlights: • A colorimetric and ratiometric fluorescent probe was developed. • The probe could detect bisulfite in PBS buffer solution and real samples. • Bisulfite test paper was made to naked-eye detect bisulfite. • This probe successfully used to living cell imaging in ratiometric manner.

  4. Colorimetric determination of Timolol concentration based on localized surface plasmon resonance of silver nanoparticles (United States)

    Amirjani, Amirmostafa; Bagheri, Mozhgan; Heydari, Mojgan; Hesaraki, Saeed


    In this work, a rapid and simple colorimetric method based on the surface plasmon resonance of silver nanoparticles (AgNPs) was developed for the detection of the drug Timolol. The method used is based on the interaction of Timolol with the surface of the as-synthesized AgNPs, which promotes aggregation of the nanoparticles. This aggregation exploits the surface plasmon resonance through the electric dipole-dipole interaction and coupling among the agglomerated particles, hence bringing forth distinctive changes in the spectra as well as the color of colloidal silver. UV-vis spectrophotometery was used to monitor the changes of the localized surface plasmon resonance of AgNPs at wavelengths of 400 and 550 nm. The developed colorimetric sensor has a wide dynamic range of 1.0 × 10-7 M-1.0 × 10-3 M for detection of Timolol with a low detection limit of 1.2 × 10-6 M. The proposed method was successfully applied for the determination of Timolol concentration in ophthalmic eye-drop solution with a response time lower than 40 s.

  5. A plasmonic colorimetric strategy for biosensing through enzyme guided growth of silver nanoparticles on gold nanostars. (United States)

    Guo, Yuehua; Wu, Jie; Li, Jie; Ju, Huangxian


    A plasmonic colorimetric strategy was designed for sensitive detection of biomolecules through enzyme guided silver nanoparticles (AgNPs) growth on gold nanostars (AuNS). The growth of AgNPs on AuNS led to a substantial blue shift of the localized surface plasmon resonance (LSPR) peak and the color change of AuNS from blue to dark blue, purple and ultimately orange. Both the LSPR blueshift wavelength and the color of detection solution containing AuNS, Ag(+) and ascorbic acid 2-phosphate (AAP) depend on the amount of enzyme that catalyzed the dephosphorylation of AAP to reduce Ag(+) on AuNS surface. Thus this strategy could be used for LSPR and naked-eye detections of both the enzyme such as alkaline phosphatase (ALP) and other biomolecules involved in biorecognition events using ALP as a tag. The LSPR detection method for ALP showed a linear range from 1.0 pM to 25 nM with a detection limit of 0.5 pM. Using DNA as a mode target molecule, this technique showed a detection range from 10 fM to 50 pM DNA with a detection limit of 2.6 fM through the convenient combination with hybridization chain reaction amplification. The proposed plasmonic colorimetric strategy could be extended as a general analytical platform for design of immunosensors and aptasensors with ALP as a label.

  6. Gold nanoparticle aggregation-based colorimetric assay for β-casein detection in bovine milk samples. (United States)

    Li, Y S; Zhou, Y; Meng, X Y; Zhang, Y Y; Song, F; Lu, S Y; Ren, H L; Hu, P; Liu, Z S; Zhang, J H


    Traditional Kjeldahl method, used for quality evaluation of bovine milk, has intrinsic defects of time-consuming sample preparation and two analyses to determine the difference between non-protein nitrogen content and total protein nitrogen content. Herein, based upon antibody functionalized gold nanoparticles (AuNPs), we described a colorimetric method for β-casein (β-CN) detection in bovine milk samples. The linear dynamic range and the LOD were 0.08-250 μg mL(-1), and 0.03 μg mL(-1) respectively. In addition, the real content of β-CN in bovine milk was measured by using the developed assay. The results are closely correlated with those from Kjeldahl method. The advantages of β-CN triggered AuNP aggregation-based colorimetric assay are simple signal generation, the high sensitivity and specificity as well as no need of complicated sample preparation, which make it for on-site detection of β-CN in bovine milk samples.

  7. Synthesis of a Phenylhydrazone-based Colorimetric Anion Sensor with Complementary IMP/INH Logic Functions

    Institute of Scientific and Technical Information of China (English)

    韦科; 熊碧; 周娜娜; 蔡苹; 胡锴; 程功臻


    A dinitrophenyl hydrazone colorimetric anion sensor (receptor 1) was synthesized and its recognition properties towards various anions were investigated by naked eye observation and spectroscopic methods, namely UV-vis and 1H NMR titrations in DMSO. The addition of AcO?, F? and H2PO4? to receptor 1 resulted in marked red shift of the charge-transfer absorbance band (Δλ=91 nm, 407 nm to 498 nm) concomitant with a 'naked-eye' detectable colour change from yellow to pink. However, both the colour and spectral changes were reversible by the addition of cations (MII) of 3d5-10 as well as CdII, HgII, MgII and CaII. Subsequently, complementary IMP/INH logic functions based on colour and spectral switching (ON/OFF) were affirmed. The sensor can, thus be utilized as a colorimetric molecular switch modulated by F?/MII.

  8. Selective and sensitive colorimetric detection of stringent alarmone ppGpp with Fenton-like reagent. (United States)

    Zheng, Lin Ling; Huang, Cheng Zhi


    Stringent alarmone, namely, guanosine 3'-diphosphate-5'-diphosphate (ppGpp), is a global regulator that plays a critical role in the survival, growth, metabolism, and many other vital processes of microorganisms. Because of its structural similarity to normal nucleotides, it is also a challenge for the selective and sensitive detection of ppGpp nowadays. Herein, we developed a colorimetric method for the selective detection of ppGpp by inhibiting the redox reaction between Fenton-like reagent (composed of Fe(3+) and H2O2) with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS). Owing to the strong coordination affinity between ppGpp and Fe(3+), the chromogenic reaction between ABTS and Fenton-like reagent, occurred in aqueous medium at 37 °C and resulted in a bluish-green solution, which was inhibited with the addition of ppGpp. This phenomenon forms the basis for the colorimetric detection of ppGpp, with a detection limit of 0.19 μM and good selectivity for ppGpp over other nucleotides and anions. Furthermore, the results could be visualized by the naked eye, and the sensitivity of the naked-eye observation could even be further improved with the aid of the introduction of a background color.

  9. An Incremental Target-Adapted Strategy for Active Geometric Calibration of Projector-Camera Systems

    Directory of Open Access Journals (Sweden)

    Hsiang-Jen Chien


    Full Text Available The calibration of a projector-camera system is an essential step toward accurate 3-D measurement and environment-aware data projection applications, such as augmented reality. In this paper we present a two-stage easy-to-deploy strategy for robust calibration of both intrinsic and extrinsic parameters of a projector. Two key components of the system are the automatic generation of projected light patterns and the incremental calibration process. Based on the incremental strategy, the calibration process first establishes a set of initial parameters, and then it upgrades these parameters incrementally using the projection and captured images of dynamically-generated calibration patterns. The scene-driven light patterns allow the system to adapt itself to the pose of the calibration target, such that the difficulty in feature detection is greatly lowered. The strategy forms a closed-loop system that performs self-correction as more and more observations become available. Compared to the conventional method, which requires a time-consuming process for the acquisition of dense pixel correspondences, the proposed method deploys a homography-based coordinate computation, allowing the calibration time to be dramatically reduced. The experimental results indicate that an improvement of 70% in reprojection errors is achievable and 95% of the calibration time can be saved.


    Directory of Open Access Journals (Sweden)

    M. Pérez


    Full Text Available For calibrating the camera, an accurate determination of the interior orientation parameters is needed. For more accurate results, the calibration images should be taken under conditions that are similar to the field samples. The aim of this work is the establishment of an efficient and accurate digital camera calibration method to be used in particular working conditions, as it can be found with our UAV (Unmanned Aerial Vehicle photogrammetric projects. The UAV used in this work was md4-200 modelled by Microdrones. The microdrone is also equipped with a standard digital non- metric camera, the Pentax Optio A40 camera. To find out the interior orientation parameters of the digital camera, two calibration methods were done. A lab calibration based on a flat pattern and a field calibration were fulfilled. To carry out the calibration, Photomodeler Scanner software was used in both cases. The lab calibration process was completely automatic using a calibration grid. The focal length was fixed at widest angle and the network included a total of twelve images with± 90º roll angles. In order to develop the field calibration, a flight plan was programmed including a total of twelve images. In the same way as in the lab calibration, the focal length was fixed at widest angle. The field test used in the study was a flat surface located on the University of Almería campus and a set of 67 target points were placed. The calibration field area was 25 × 25 m approximately and the altitude flight over ground was 50 m. After the software processing, the camera calibration parameter values were obtained. The paper presents the process, the results and the accuracy of these calibration methods. The field calibration method reduced the final total error obtained in the previous lab calibration. Furthermore the overall RMSs obtained from both methods are similar. Therefore we will apply the field calibration results to all our photogrammetric projects in which

  11. Calibration of "Babyline" RP instruments

    CERN Multimedia


      If you have old RP instrumentation of the “Babyline” type, as shown in the photo, please contact the Radiation Protection Group (Joffrey Germa, 73171) to have the instrument checked and calibrated. Thank you. Radiation Protection Group

  12. Field calibration of cup anemometers

    Energy Technology Data Exchange (ETDEWEB)

    Kristensen, L.; Jensen, G.; Hansen, A.; Kirkegaard, P.


    An outdoor calibration facility for cup anemometers, where the signals from 10 anemometers of which at least one is a reference can be recorded simultaneously, has been established. The results are discussed with special emphasis on the statistical significance of the calibration expressions. It is concluded that the method has the advantage that many anemometers can be calibrated accurately with a minimum of work and cost. The obvious disadvantage is that the calibration of a set of anemometers may take more than one month in order to have wind speeds covering a sufficiently large magnitude range in a wind direction sector where we can be sure that the instruments are exposed to identical, simultaneous wind flows. Another main conclusion is that statistical uncertainty must be carefully evaluated since the individual 10 minute wind-speed averages are not statistically independent. (au)

  13. Infrasound Sensor Calibration and Response (United States)


    functions with faster rise times. SUMMARY We have documented past work on the determination of the calibration constant of the LANL infrasound sensor...Monitoring Technologies 735 Report Documentation Page Form ApprovedOMB No. 0704-0188 Public reporting burden for the collection of information is estimated...National Laboratory ( LANL ) has operated an infrasound sensor calibration chamber that operates over a frequency range of 0.02 to 4 Hz. This chamber has

  14. Bayesian Calibration of Microsimulation Models. (United States)

    Rutter, Carolyn M; Miglioretti, Diana L; Savarino, James E


    Microsimulation models that describe disease processes synthesize information from multiple sources and can be used to estimate the effects of screening and treatment on cancer incidence and mortality at a population level. These models are characterized by simulation of individual event histories for an idealized population of interest. Microsimulation models are complex and invariably include parameters that are not well informed by existing data. Therefore, a key component of model development is the choice of parameter values. Microsimulation model parameter values are selected to reproduce expected or known results though the process of model calibration. Calibration may be done by perturbing model parameters one at a time or by using a search algorithm. As an alternative, we propose a Bayesian method to calibrate microsimulation models that uses Markov chain Monte Carlo. We show that this approach converges to the target distribution and use a simulation study to demonstrate its finite-sample performance. Although computationally intensive, this approach has several advantages over previously proposed methods, including the use of statistical criteria to select parameter values, simultaneous calibration of multiple parameters to multiple data sources, incorporation of information via prior distributions, description of parameter identifiability, and the ability to obtain interval estimates of model parameters. We develop a microsimulation model for colorectal cancer and use our proposed method to calibrate model parameters. The microsimulation model provides a good fit to the calibration data. We find evidence that some parameters are identified primarily through prior distributions. Our results underscore the need to incorporate multiple sources of variability (i.e., due to calibration data, unknown parameters, and estimated parameters and predicted values) when calibrating and applying microsimulation models.

  15. Pressures Detector Calibration and Measurement

    CERN Document Server



    This is report of my first and second projects (of 3) in NA61. I did data taking and analysis in order to do calibration of pressure detectors and verified it. I analyzed the data by ROOT software using the C ++ programming language. The first part of my project was determination of calibration factor of pressure sensors. Based on that result, I examined the relation between pressure drop, gas flow rate of in paper filter and its diameter.

  16. Beam Imaging and Luminosity Calibration

    CERN Document Server

    Klute, Markus; Salfeld-Nebgen, Jakob


    We discuss a method to reconstruct two-dimensional proton bunch densities using vertex distributions accumulated during LHC beam-beam scans. The $x$-$y$ correlations in the beam shapes are studied and an alternative luminosity calibration technique is introduced. We demonstrate the method on simulated beam-beam scans and estimate the uncertainty on the luminosity calibration associated to the beam-shape reconstruction to be below 1\\%.

  17. Annual review in automatic programming

    CERN Document Server

    Goodman, Richard


    Annual Review in Automatic Programming focuses on the techniques of automatic programming used with digital computers. Topics covered range from the design of machine-independent programming languages to the use of recursive procedures in ALGOL 60. A multi-pass translation scheme for ALGOL 60 is described, along with some commercial source languages. The structure and use of the syntax-directed compiler is also considered.Comprised of 12 chapters, this volume begins with a discussion on the basic ideas involved in the description of a computing process as a program for a computer, expressed in

  18. Algorithms for skiascopy measurement automatization (United States)

    Fomins, Sergejs; Trukša, Renārs; KrūmiĆa, Gunta


    Automatic dynamic infrared retinoscope was developed, which allows to run procedure at a much higher rate. Our system uses a USB image sensor with up to 180 Hz refresh rate equipped with a long focus objective and 850 nm infrared light emitting diode as light source. Two servo motors driven by microprocessor control the rotation of semitransparent mirror and motion of retinoscope chassis. Image of eye pupil reflex is captured via software and analyzed along the horizontal plane. Algorithm for automatic accommodative state analysis is developed based on the intensity changes of the fundus reflex.

  19. Automatic Construction of Finite Algebras

    Institute of Scientific and Technical Information of China (English)



    This paper deals with model generation for equational theories,i.e.,automatically generating (finite)models of a given set of (logical) equations.Our method of finite model generation and a tool for automatic construction of finite algebras is described.Some examples are given to show the applications of our program.We argue that,the combination of model generators and theorem provers enables us to get a better understanding of logical theories.A brief comparison betwween our tool and other similar tools is also presented.

  20. Automatic mapping of monitoring data

    DEFF Research Database (Denmark)

    Lophaven, Søren; Nielsen, Hans Bruun; Søndergaard, Jacob


    This paper presents an approach, based on universal kriging, for automatic mapping of monitoring data. The performance of the mapping approach is tested on two data-sets containing daily mean gamma dose rates in Germany reported by means of the national automatic monitoring network (IMIS......). In the second dataset an accidental release of radioactivity in the environment was simulated in the South-Western corner of the monitored area. The approach has a tendency to smooth the actual data values, and therefore it underestimates extreme values, as seen in the second dataset. However, it is capable...

  1. A simple readout electronics for automatic power controlled self-mixing laser diode systems. (United States)

    Cattini, Stefano; Rovati, Luigi


    The paper describes a simple electronic circuit to drive a laser diode for self-mixing interferometry. The network integrates a stable commercial automatic power controller and a current mirror based readout of the interferometric signal. The first prototype version of the circuit has been realized and characterized. The system allows easily performing precise interferometric measurements with no thermostatic circuitry to stabilize the laser diode temperature and an automatic control gain network to compensate emitted optical power fluctuations. To achieve this result, in the paper a specific calibration procedure to be performed is described.

  2. Self-Calibrating Surface Measuring Machine (United States)

    Greenleaf, Allen H.


    A new kind of surface-measuring machine has been developed under government contract at Itek Optical Systems, a Division of Itek Corporation, to assist in the fabrication of large, highly aspheric optical elements. The machine uses four steerable distance-measuring interferometers at the corners of a tetrahedron to measure the positions of a retroreflective target placed at various locations against the surface being measured. Using four interferometers gives redundant information so that, from a set of measurement data, the dimensions of the machine as well as the coordinates of the measurement points can be determined. The machine is, therefore, self-calibrating and does not require a structure made to high accuracy. A wood-structured prototype of this machine was made whose key components are a simple form of air bearing steering mirror, a wide-angle cat's eye retroreflector used as the movable target, and tracking sensors and servos to provide automatic tracking of the cat's eye by the four laser beams. The data are taken and analyzed by computer. The output is given in terms of error relative to an equation of the desired surface. In tests of this machine, measurements of a 0.7 m diameter mirror blank have been made with an accuracy on the order of 0.2µm rms.

  3. A BTT-Based Colorimetric Dual Sensor for Hg(II and Selected Anions with Molecular Logic Operations

    Directory of Open Access Journals (Sweden)

    Veikko Uahengo


    Full Text Available A benzotrithiophenes (BTT based colorimetric sensor (2 was synthesized via a simple one-step reaction process, and its recognition properties towards several biologically important anions and cations were investigated by naked eye observation and spectroscopic methods of UV-vis and 1H NMR analysis in DMSO. The molar addition of anions such as F−, AcO−, and H2PO4- induced a marked red shift of the charge transfer band (Δλ=94 nm, from 440 nm to 534 nm concomitant with a clearly visible “naked eye” detectable colorimetric activities (from orange to pink. However, the molar addition of H2PO4- did not induce significant spectral changes, compared to F− and AcO−. Furthermore, the molar addition of Hg2+ to 2 resulted in hypochromic shift at 440 nm and the eventual disappearance of a low energy band at 534 nm, concomitant with the colorimetric activities (from orange to yellow. Moreover, both spectral and color changes induced by the addition of anions (F− and AcO− were all reversible by the molar addition of metal cations of 3d5–10. The reversibility properties triggered the logic operation studies, and, subsequently, the complementary IMP/INH logic functions based on color and spectral switching (ON/OFF were confirmed. Conclusively, 2 can thus be utilized as a colorimetric molecular switch modulated by AcO−/MII.

  4. A New Colorimetric Assay of Tabletop Sweeteners Using a Modified Biuret Reagent: An Analytical Chemistry Experiment for the Undergraduate Curriculum (United States)

    Fenk, Christopher J.; Kaufman, Nathan; Gerbig, Donald G., Jr.


    A new, fast and effective colorimetric analysis of the artificial sweetener aspartame is presented for application in undergraduate laboratory courses. This new method incorporates the use of a modified biuret reagent for selective detection and analysis of aspartame in aqueous solutions. The modified reagent is less caustic than the traditional…

  5. Evaluation of micro-colorimetric lipid determination method with samples prepared using sonication and accelerated solvent extraction methods (United States)

    Two common laboratory extraction techniques were evaluated for routine use with the micro-colorimetric lipid determination method developed by Van Handel (1985) [E. Van Handel, J. Am. Mosq. Control Assoc. 1(1985) 302] and recently validated for small samples by Inouye and Lotufo ...

  6. Highly sensitive colorimetric detection of glucose and uric acid in biological fluids using chitosan-modified paper microfluidic devices. (United States)

    Gabriel, Ellen F M; Garcia, Paulo T; Cardoso, Thiago M G; Lopes, Flavio M; Martins, Felipe T; Coltro, Wendell K T


    This paper describes the modification of microfluidic paper-based analytical devices (μPADs) with chitosan to improve the analytical performance of colorimetric measurements associated with enzymatic bioassays. Chitosan is a natural biopolymer extensively used to modify biosensing surfaces due to its capability of providing a suitable microenvironment for the direct electron transfer between an enzyme and a reactive surface. This hypothesis was investigated using glucose and uric acid (UA) colorimetric assays as model systems. The best colorimetric sensitivity for glucose and UA was achieved using a chromogenic solution composed of 4-aminoantipyrine and sodium 3,5-dichloro-2-hydroxy-benzenesulfonate (4-AAP/DHBS), which provided a linear response for a concentration range between 0.1 and 1.0 mM. Glucose and UA were successfully determined in artificial serum samples with accuracies between 87 and 114%. The limits of detection (LODs) found for glucose and UA assays were 23 and 37 μM, respectively. The enhanced analytical performance of chitosan-modified μPADs allowed the colorimetric detection of glucose in tear samples from four nondiabetic patients. The achieved concentration levels ranged from 130 to 380 μM. The modified μPADs offered analytical reliability and accuracy as well as no statistical difference from the values achieved through a reference method. Based on the presented results, the proposed μPAD can be a powerful alternative tool for non-invasive glucose analysis.

  7. Evaluation of direct colorimetric MTT assay for rapid detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis

    NARCIS (Netherlands)

    G.B. Hundie (Gadissa Bedada); Woldemeskel, D. (Dawit); Gessesse, A. (Amare)


    textabstractWith the spread of multidrug-resistant tuberculosis (MDR-TB) strains there is an increasing need for new accurate and cost-effective methods for a rapid diagnostic and drug susceptibility testing (DST), particularly in low-income countries where tuberculosis is hyperendemic. A colorimetr

  8. Automatic quantification of iris color

    DEFF Research Database (Denmark)

    Christoffersen, S.; Harder, Stine; Andersen, J. D.;


    An automatic algorithm to quantify the eye colour and structural information from standard hi-resolution photos of the human iris has been developed. Initially, the major structures in the eye region are identified including the pupil, iris, sclera, and eyelashes. Based on this segmentation, the ...

  9. Trevi Park: Automatic Parking System


    ECT Team, Purdue


    TreviPark is an underground, multi-story stacking system that holds cars efficiently, thus reducing the cost of each parking space, as a fully automatic parking system intended to maximize space utilization in parking structures. TreviPark costs less than the price of a conventional urban garage and takes up half the volume and 80% of the depth.

  10. Automatic agar tray inoculation device (United States)

    Wilkins, J. R.; Mills, S. M.


    Automatic agar tray inoculation device is simple in design and foolproof in operation. It employs either conventional inoculating loop or cotton swab for uniform inoculation of agar media, and it allows technician to carry on with other activities while tray is being inoculated.

  11. Automatic Error Analysis Using Intervals (United States)

    Rothwell, E. J.; Cloud, M. J.


    A technique for automatic error analysis using interval mathematics is introduced. A comparison to standard error propagation methods shows that in cases involving complicated formulas, the interval approach gives comparable error estimates with much less effort. Several examples are considered, and numerical errors are computed using the INTLAB…

  12. Automatic Validation of Protocol Narration

    DEFF Research Database (Denmark)

    Bodei, Chiara; Buchholtz, Mikael; Degano, Pierpablo;


    We perform a systematic expansion of protocol narrations into terms of a process algebra in order to make precise some of the detailed checks that need to be made in a protocol. We then apply static analysis technology to develop an automatic validation procedure for protocols. Finally, we...

  13. Automatic Identification of Metaphoric Utterances (United States)

    Dunn, Jonathan Edwin


    This dissertation analyzes the problem of metaphor identification in linguistic and computational semantics, considering both manual and automatic approaches. It describes a manual approach to metaphor identification, the Metaphoricity Measurement Procedure (MMP), and compares this approach with other manual approaches. The dissertation then…

  14. Automatic milking : a better understanding

    NARCIS (Netherlands)

    Meijering, A.; Hogeveen, H.; Koning, de C.J.A.M.


    In 2000 the book Robotic Milking, reflecting the proceedings of an International Symposium which was held in The Netherlands came out. At that time, commercial introduction of automatic milking systems was no longer obstructed by technological inadequacies. Particularly in a few west-European countr

  15. Discovering the enzyme mimetic activity of metal-organic framework (MOF) for label-free and colorimetric sensing of biomolecules. (United States)

    Wang, Ying; Zhu, Yingjing; Binyam, Atsebeha; Liu, Misha; Wu, Yinan; Li, Fengting


    A label-free sensing strategy based on the enzyme-mimicking activity of MOF was demonstrated for colorimetric detection of biomolecules. Firstly obvious blue color was observed due to the high efficiency of peroxidase-like catalytic activity of Fe-MIL-88A (an ion-based MOF material) toward 3,3',5,5'-tetramethylbenzidine (TMB). Then in the presence of target biomolecule and corresponding aptamer, the mimetic activity of Fe-MIL-88A can be strongly inhibited and used directly to realize the colorimetric detection. On the basis of the interesting findings, we designed a straightforward, label-free and sensitive colorimetric method for biomolecule detection by using the enzyme mimetic property of MOF coupling with molecular recognition element. Compared with the existed publications, our work breaks the routine way by setting up an inorganic-organic MOF-aptamer hybrid platform for colorimetric determination of biomolecules, expanding the targets scope from H2O2 or glucose to biomolecules. As a proof of concept, thrombin and thrombin aptamer was used as a model analyte. The limit of detection of 10nM can be achieved with naked eyes and ultrahigh selectivity of thrombin toward numerous interfering substances with 10-fold concentration was demonstrated significantly. Of note, the method was further applied for the detection of thrombin in human serum samples, showing the results in agreement with those values obtained in an immobilization buffer by the colorimetric method. This inorganic-organic MOF-aptamer sensing strategy may in principle be universally applicable for the detection of a range of environmental or biomedical molecules of interests.

  16. Dual-Modal Colorimetric/Fluorescence Molecular Probe for Ratiometric Sensing of pH and Its Application. (United States)

    Wu, Luling; Li, Xiaolin; Huang, Chusen; Jia, Nengqin


    As traditional pH meters cannot work well for minute regions (such as subcellular organelles) and in harsh media, molecular pH-sensitive devices for monitoring pH changes in diverse local heterogeneous environments are urgently needed. Here, we report a new dual-modal colorimetric/fluorescence merocyanine-based molecular probe (CPH) for ratiometric sensing of pH. Compared with previously reported pH probes, CPH bearing the benzyl group at the nitrogen position of the indolium group and the phenol, which is used as the acceptor for proton, could respond to pH changes immediately through both the ratiometric fluorescence signal readout and naked-eye colorimetric observation. The sensing process was highly stable and reversible. Most importantly, the suitable pKa value (6.44) allows CPH to presumably accumulate in lysosomes and become a lysosome-target fluorescent probe. By using CPH, the intralysosomal pH fluctuation stimulated by antimalaria drug chloroquine was successfully tracked in live cells through the ratiometric fluorescence images. Additionally, CPH could be immobilized on test papers, which exhibited a rapid and reversible colorimetric response to acid/base vapor through the naked-eye colorimetric analysis. This proof-of-concept study presents the potential application of CPH as a molecular tool for monitoring intralysosomal pH fluctuation in live cells, as well as paves the way for developing the economic, reusable, and fast-response optical pH meters for colorimetric sensing acid/base vapor with direct naked-eye observation.

  17. A self-calibrating robot based upon a virtual machine model of parallel kinematics

    DEFF Research Database (Denmark)

    Pedersen, David Bue; Eiríksson, Eyþór Rúnar; Hansen, Hans Nørgaard


    a virtual machine of the kinematics system, built on principles from geometrical metrology. Relevant mathematically non-trivial deviations to the ideal machine are identified and decomposed into elemental deviations. From these deviations, a routine is added to a physical machine tool, which allows...... it to recognise its own geometry by probing the vertical offset from tool point to the machine table, at positions in the horizontal plane. After automatic calibration the positioning error of the machine tool was reduced from an initial error after its assembly of ±170 µm to a calibrated error of ±3 µm...

  18. Colorimetric evaluation of three adhesives used in the consolidation of contemporary matte paint after artificial ageing

    Directory of Open Access Journals (Sweden)

    Rosario Llamas


    Full Text Available Of all the problems associated to contemporary painting, the most complex is probably that which concerns powdering matte surfaces, both for their technical characteristics and optical properties and for the aesthetic significance associated to these types of finishes. These pictorial surfaces are technically complex due to the high risk of irreversible alterations associated to the appearance of the treated surface and the potential for streaking, tide lines, changes in colour, darkening, added gloss and changes in texture during the treatment process. For this study, a colorimetric analysis was performed to evaluate the behaviour of three adhesives commonly used in the consolidation of these types of painted surfaces, to determine the effect of ageing on the adhesives and quantify their stability as a function of adhesive type, concentration, and application method. Of all the adhesives, funori did not result in significant changes when aged using this method.

  19. A plasmonic colorimetric strategy for visual miRNA detection based on hybridization chain reaction (United States)

    Miao, Jie; Wang, Jingsheng; Guo, Jinyang; Gao, Huiguang; Han, Kun; Jiang, Chengmin; Miao, Peng


    In this work, a novel colorimetric strategy for miRNA analysis is proposed based on hybridization chain reaction (HCR)-mediated localized surface plasmon resonance (LSPR) variation of silver nanoparticles (AgNPs). miRNA in the sample to be tested is able to release HCR initiator from a solid interface to AgNPs colloid system by toehold exchange-mediated strand displacement, which then triggers the consumption of fuel strands with single-stranded tails for HCR. The final produced long nicked double-stranded DNA loses the ability to protect AgNPs from salt-induced aggregation. The stability variation of the colloid system can then be monitored by recording corresponding UV-vis spectrum and initial miRNA level is thus determined. This sensing system involves only four DNA strands which is quite simple. The practical utility is confirmed to be excellent by employing different biological samples.

  20. Anion Sensing Properties of New Colorimetric Chemosensors Based on Thiourea and Urea Moieties

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Dong Wan; Kim, Jung Hwan; Hwang, Jae Young; Park, Jong Keun; Kim, Jae Sang [Gyeongsang National University, Jinju (Korea, Republic of)


    A new colorimetric sensors containing thiourea (1-(4-nitrophenyl)-3-quinolin-6-ylthiourea: 1) and urea(1-(4- nitrophenyl)-3-quinolin-6-ylurea: 2) moieties for fluoride were designed and synthesized. These simple receptors were characterized their stoichiometry, and investigates the mechanism of their selectivity as anion receptors. The addition of tetrabutylammonium fluoride salts to the solution of receptors caused a dramatically and clearly observable color changes from colorless to yellow. To examine their application as anion receptors by UV-vis and {sup 1}H NMR spectroscopy results revealed their higher selectivity for fluoride ion than other anions. The receptors and fluoride ion formed a 1:1 stoichiometry complex through strong hydrogen bonding interactions in the first step, followed by a process of deprotonation in presence of an excess of F{sup -} in DMSO solvent

  1. In vitro preliminary cytotoxicity testing of vegetal extracts, using colorimetric methods

    Directory of Open Access Journals (Sweden)

    Claudia Patricia Cordero Camacho


    Full Text Available To advance in the study of the Colombian vegetal biodiversity, considered as a potential source of pharmacologically active products, the establishment of biological activity evaluation systems is necessary, which allow the detection of active products against pathologies with high social and economical impact, such as cancer. This work describes the implementation of a preliminary in vitro methodology for the determination of potential anticancer activity in vegetal extracts, by cytotoxicity testing upon human tumor cell lines, measuring the cellular mass indirectly with the colorimetric assays of MTT (methyl tetrazolium tiazole reduction and SRB (sulforhodamine Bstaining. HT-29, MCF-7, SiHa and HEp-2 cell lines cultures were adapted, MTT concentration, cellular density and treatment period parameters for the cytotoxicity assay were selected. Cell lines sensitivity to the chemotherapeutic agent Doxorubicin HCl was determined. Colombian vegetal species extracts cytotoxicity was tested and usefulness of the assay as a tool to bioguide the search of active products was evidenced.

  2. Citric acid-coated gold nanoparticles for visual colorimetric recognition of pesticide dimethoate (United States)

    Dar, Aqib Iqbal; Walia, Shanka; Acharya, Amitabha


    A colorimetric chemo-sensor based on citric acid-coated gold NPs (C-GNP) showed a linear increase in fluorescence intensity with increasing concentration of pesticide dimethoate (DM). The limit of detection was found to be between 8.25± 0.3 and 20 ± 9.5 ppm. The increase in fluorescence intensity was suggested to have originated from the soft-soft interaction between C-GNPs and DM via sulfur group which is absent in pesticide dicofol (DF). Similar studies with citric acid-coated silver NPs (C-SNPs) did not result any change in the fluorescence intensity. The microscopic studies suggested aggregation of C-GNPs in the presence of DM but not in case of DF.

  3. Aloe vera is non-toxic to cells: A microculture tetrazolium technique colorimetric assay study

    Directory of Open Access Journals (Sweden)

    Devi Gopakumar


    Full Text Available Introduction: Aloe vera (Av, a succulent of Liliaceae family is now a widely used medicinal plant. Its′ application covers a wide spectrum of human diseases, including oral mucosa, gastric mucosa and skin. Aloe vera preparations in the form of gel, mouth washes and cream are applied topically for many oral diseases. The applications include oral lichen planus, candidiasis, oral submucous fibrosis, geographic tongue, etc. Aims and Objectives: To evaluate the cytotoxicity of Av on human fibroblasts. Materials and Methods: Aloe vera preparation (70% was applied on the fibroblast cell lineage and the cell viability was evaluated by microculture tetrazolium technique (MTT colorimetric assay. Results: The cell viability at different concentrations was measured. The cells have maintained their viability at different concentrations used in the study. Conclusion: Our study shows the cell viability at different sample concentrations of Av. This could open up wide clinical applications of Av for reactive, inflammatory and potentially malignant oral and other mucocutaneous diseases.

  4. Optimisation of Arsenic (III by Colorimetric Incorporated with Image Processing Technique

    Directory of Open Access Journals (Sweden)

    Jin Hoong Leong


    Full Text Available Inorganic arsenic contamination has caused a remarkable impact on the contamination of soil and groundwater in many counties. Consequently, determination of inorganic arsenic on site is very crucial especially arsenic (III which is more toxic than arsenic (V. Thus, a more rapid, simple and ecofriendly approach was developed in this study to determine arsenic (III by incorporation of image processing technique into colorimetric method. The effects of various factors were evaluated by a 24 full factorial design with a blocking factor. The mass ratio of sulfamic acid to zinc powder was the most significant factor affected red, green and blue (RGB color values and followed by reaction period. The optimum conditions for the detection were found to be using 1 g of sulfamic acid and 0.5 g of zinc powder at 5 minutes. This work also demonstrates that the developed method is able to detect arsenic (III rapidly and easily.

  5. Colorimetric characterization of liquid crystal display using an improved two-stage model

    Institute of Scientific and Technical Information of China (English)

    Yong Wang; Haisong Xu


    @@ An improved two-stage model of colorimetric characterization for liquid crystal display (LCD) was proposed. The model included an S-shape nonlinear function with four coefficients for each channel to fit the Tone reproduction curve (TRC), and a linear transfer matrix with black-level correction. To compare with the simple model (SM), gain-offset-gain (GOG), S-curve and three-one-dimensional look-up tables (3-1D LUTs) models, an identical LCD was characterized and the color differences were calculated and summarized using the set of 7 × 7 × 7 digital-to-analog converter (DAC) triplets as test data. The experimental results showed that the model was outperformed in comparison with the GOG and SM ones, and near to that of the S-curve model and 3-1D LUTs method.

  6. Sensing behavior and logic operation of a colorimetric fluorescence sensor for Hg2 +/Cu2 + ions (United States)

    He, Tian; Lin, Cuiling; Gu, Zhengye; Xu, Luonan; Yang, Anle; Liu, Yuanyuan; Fang, Huajun; Qiu, Huayu; Zhang, Jing; Yin, Shouchun


    A BODIPY-based 1 as a colorimetric fluorescence sensor was synthesized, and its metal sensing property was investigated. 1 displayed high selectivity and sensitivity towards Hg2 + and Cu2 + ions among 15 different metal cations. The addition of Hg2 + and Cu2 + ions into 1 in CH3CN resulted in a significant bathochromic shift of the UV absorption spectra from 533 nm to 560 nm and 593 nm, respectively, changing the corresponding colors from pink to purple and blue. When excited at 530 nm, the fluorescence intensity of 1 was quenched over 75% upon addition of Hg2 + ions, while 1 with Cu2 + ions exhibited significant fluorescence enhancement with a 23 nm red-shift. Based on these results, three logic gates (OR, IMPLICATION, and INHIBIT) were obtained by controlling the chemical inputs.

  7. Colorimetric Sensors for Anion Recognition Based on the Proton Transfer Signaling Mechanism

    Institute of Scientific and Technical Information of China (English)

    HUANG Xiaohuan; HE Yongbing; CHEN Zhihong; HU Chenguang


    Phenolic hydroxyl based sensors N,N-bi(salicylidene)-1,2-phenylenediamine (1),2-[(4-nitrophenylimino)methyl] phenol (2) and 2-[p-tdylimino methyl] phenol (3) bearing Schiff-base groups can act as selective colorimetric sensors for anions,which exhibit distinct color changes in the presence of fluoride,but show no response to other halogen anions.They also give response to acetate,which is clearly visible to the naked eyes.The selectivity can be rationalized on the proton transfer signaling mechanism.The sensor 1 containing plural phenolic groups undergoes stepwise deprotonation of the two O-H fragments when it interacts with excess fluoride,which is confirmed by UV-Vis,1H NMR and 19F NMR spectroscopic methods.

  8. Experimental study of boundary layers formation by thin film colorimetric interferometry

    Institute of Scientific and Technical Information of China (English)

    MartinHartl; IvanKrupka; MiroslavLiska


    Thin film colorimetric interferometry was applied to the preliminary study of boundarylayers formation for a several liquids of known molecular structure that have been previously stud-ied by the force balance method. This technique intended for the study of very thin lubrication filmsdown to a few nanometers in a point contact between a steel ball and a transparent disk combinespowerful film thickness mapping capabilities with high accuracy. Central and minimum film thick-ness as well as film shape in the dependence on rolling speed was studied for hexadecane, oc-tamethylcyclotetrasiloxane (OMCTS) and n-tetradecane. Results are compared with data obtainedfrom surface force apparatus measurements. OMCTS and n-tetradecane were found to formboundary films that result in a considerable enhancement in film thickness at slow speeds.

  9. Detecting Optic Atrophy in Multiple Sclerosis Patients Using New Colorimetric Analysis Software: From Idea to Application. (United States)

    Bambo, Maria Pilar; Garcia-Martin, Elena; Perez-Olivan, Susana; Larrosa-Povés, José Manuel; Polo-Llorens, Vicente; Gonzalez-De la Rosa, Manuel


    Neuro-ophthalmologists typically observe a temporal pallor of the optic disc in patients with multiple sclerosis. Here, we describe the emergence of an idea to quantify these optic disc color changes in multiple sclerosis patients. We recruited 12 multiple sclerosis patients with previous optic neuritis attack and obtained photographs of their optic discs. The Laguna ONhE, a new colorimetric software using hemoglobin as the reference pigment in the papilla, was used for the analysis. The papilla of these multiple sclerosis patients showed greater pallor, especially in the temporal sector. The software detected the pallor and assigned hemoglobin percentages below normal reference values. Measurements of optic disc hemoglobin levels obtained with the Laguna ONhE software program had good ability to detect optic atrophy and, consequently, axonal loss in multiple sclerosis patients. This new technology is easy to implement in routine clinical practice.

  10. Colorimetric As (V) detection based on S-layer functionalized gold nanoparticles. (United States)

    Lakatos, Mathias; Matys, Sabine; Raff, Johannes; Pompe, Wolfgang


    Herein, we present simple and rapid colorimetric and UV/VIS spectroscopic methods for detecting anionic arsenic (V) complexes in aqueous media. The methods exploit the aggregation of S-layer-functionalized spherical gold nanoparticles of sizes between 20 and 50 nm in the presence of arsenic species. The gold nanoparticles were functionalized with oligomers of the S-layer protein of Lysinibacillus sphaericus JG-A12. The aggregation of the nanoparticles results in a color change from burgundy-red for widely dispersed nanoparticles to blue for aggregated nanoparticles. A detailed signal analysis was achieved by measuring the shift of the particle plasmon resonance signal with UV/VIS spectroscopy. To further improve signal sensitivity, the influence of larger nanoparticles was tested. In the case of 50 nm gold nanoparticles, a concentration of the anionic arsenic (V) complex lower than 24 ppb was detectable.

  11. Colorimetric detection of biological hydrogen sulfide using fluorosurfactant functionalized gold nanorods. (United States)

    Zhang, Xuan; Zhou, Wenjuan; Yuan, Zhiqin; Lu, Chao


    As a well-known environmental pollutant but also an important gaseous transmitter, the specific detection of hydrogen sulfide (H2S) is significant in biological systems. In this study, fluorosurfactant functionalized gold nanorods (FSN-AuNRs) have been proposed to act as selective colorimetric nanoprobes for H2S. With the combination of strong gold-S interactions and small FSN bilayer interstices, FSN-AuNRs demonstrate favorable selectivity and sensitivity toward H2S over other anions and small biological molecules. The practical application of the present method in biological H2S detection was validated with human and mouse serum samples. Moreover, the proposed nanoprobe can also be used for evaluating the activity of H2S synthetase.

  12. Gold Nanoparticle-based Colorimetric Sensor for pH Sensing

    Institute of Scientific and Technical Information of China (English)

    ZHU Lin; XUE Dong-hua; WANG Zhen-xin


    We present a newly designed colorimetric sensor sensitive to pH value based on a gold nanocomposite composed of gold nanoparticles and pH-sensitive polymer{dodecylthioether end functionalized poly[2-(diethylamino)ethyl methacrylate],poly(DEAEMA)-DDT}.We have shown that this design can produce stable GNP precipitate under weakly basic condition(pH=7.5) and this precipitate can be dispersed in acidic solution(pH=4.0),due to the 2-(diethylamino) ethyl methacrylate protonated by H+.The precipitation-dispersion process is reversible and accompanied by distinct color change that are clearly visible to the naked eye.This development may have application in the controlled fabrication of reversible pH-sensitive nanostructures and/or devices.

  13. Hierarchically structured photonic crystals for integrated chemical separation and colorimetric detection. (United States)

    Fu, Qianqian; Zhu, Biting; Ge, Jianping


    A SiO2 colloidal photonic crystal film with a hierarchical porous structure is fabricated to demonstrate an integrated separation and colorimetric detection of chemical species for the first time. This new photonic crystal based thin layer chromatography process requires no dyeing, developing and UV irradiation compared to the traditional TLC. The assembling of mesoporous SiO2 particles via a supersaturation-induced-precipitation process forms uniform and hierarchical photonic crystals with micron-scale cracks and mesopores, which accelerate the diffusion of developers and intensify the adsorption/desorption between the analytes and silica for efficient separation. Meanwhile, the chemical substances infiltrated to the voids of photonic crystals cause an increase of the refractive index and a large contrast of structural colors towards the unloaded part, so that the sample spots can be directly recognized with the naked eye before and after separation.

  14. Acylthiourea derivatives as colorimetric sensors for anions: Synthesis, characterization and spectral behaviors. (United States)

    Liu, Shuangshuang; Kang, Jing; Cao, Xiufang; Yue, Xiali


    Several acylthioureas have been synthesized to develop colorimetric sensors for detection of biologically important anions. UV-vis titration experiments indicated that the absorbance values have a good linear relationship with concentration of anions when the anions were added in AR-1, AR-4 and AR-6 sensor molecules. The detection limit to AcO(-) and F(-) is 5×10(-6) mol/L when the concentration of receptors are 2×10(-5) mol/L. Especially, compounds AR-1 and AR-4, decorated with strong electron-withdrawing NO2 substituent, showed augmented anion sensing properties, being capable of naked-eye detecting of F(-) and AcO(-) when the water content is lower than 15%. The recognition details of anion sensing were also assessed using (1)H NMR technique and confirmed that the basic anions induced deprotonation of N-H.

  15. Colorimetric monitoring of solid-phase aldehydes using 2,4-dinitrophenylhydrazine. (United States)

    Shannon, Simon K; Barany, George


    A simple and rapid method to achieve colorimetric monitoring of resin-bound aldehydes, based on ambient temperature reaction with 2,4-dinitrophenylhydrazine (DNPH) in the presence of dilute acid, has been developed as an adjunct to solid-phase organic synthesis and combinatorial chemistry. By this test, the presence of aldehydes is indicated by a red to dark-orange appearance, within a minute. Alternatively, resins that are free of aldehydes or in which aldehyde functions have reacted completely retain their original color. The DNPH test was demonstrated for poly(ethylene glycol)-polystyrene (PEG-PS), aminomethyl polystyrene (AMP), cross-linked ethoxylate acrylate resin (CLEAR), and acryloylated O,O'-bis(2-aminopropyl)poly(ethylene glycol) (PEGA) supports and gave results visible to the naked eye at levels as low as 18 micromol of aldehyde per gram of resin.

  16. A coumarin-indole based colorimetric and 'turn on' fluorescent probe for cyanide (United States)

    Xu, Yu; Dai, Xi; Zhao, Bao-Xiang


    A novel coumarin-indole based chemodosimeter with a simple structure was designed and prepared via a condensation reaction in high yield. The probe exhibited very high selectivity towards cyanide on both fluorescence and UV-vis spectra, which allowed it to quantitatively detect and imaging cyanide ions in organic-aqueous solution by either fluorescence enhancement or colorimetric changes. Confirmed by 1H NMR and HRMS spectra, the detection mechanism was proved to be related with the Michael addition reaction induced by cyanide ions, which blocked the intramolecular charge transfer (ICT) of the probe. Moreover, the probe was able to be utilized efficiently in a wide pH range (7.5-10) with negligible interference from other anions and a low detection limit of 0.51 μM. Application in 5 kinds of natural water source and accurate detection of cyanide in tap water solvent system also indicated the high practical significance of the probe.

  17. Colorimetric Solid Phase Extraction (CSPE): Using Color to Monitor Spacecraft Water Quality (United States)

    Gazda, Daniel B.; Nolan, Daniel J.; Rutz, Jeffrey A.; Schultz, John R.; Siperko, Lorraine M.; Porter, Marc D.; Lipert, Robert J.; Flint, Stephanie M.; McCoy, J. Torin


    In August 2009, an experimental water quality monitoring kit based on Colorimetric Solid Phase Extraction (CSPE) technology was delivered to the International Space Station (ISS). The kit, called the Colorimetric Water Quality Monitoring Kit (CWQMK), was launched as a Station Development Test Objective (SDTO) experiment to evaluate the suitability of CSPE technology for routine use monitoring water quality on the ISS. CSPE is a sorption-spectrophotometric technique that combines colorimetric reagents, solid-phase extraction, and diffuse reflectance spectroscopy to quantify trace analytes in water samples. In CSPE, a known volume of sample is metered through a membrane disk that has been impregnated with an analyte-specific colorimetric reagent and any additives required to optimize the formation of the analyte-reagent complex. As the sample flows through the membrane disk, the target analyte is selectively extracted, concentrated, and complexed. Formation of the analyte-reagent complex causes a detectable change in the color of the membrane disk that is proportional to the amount of analyte present in the sample. The analyte is then quantified by measuring the color of the membrane disk surface using a hand-held diffuse reflectance spectrophotometer (DRS). The CWQMK provides the capability to measure the ionic silver (Ag +) and molecular iodine (I2) in water samples on-orbit. These analytes were selected for the evaluation of CSPE technology because they are the biocides used in the potable water storage and distribution systems on the ISS. Biocides are added to the potable water systems on spacecraft to inhibit microbial growth. On the United States (US) segment of the ISS molecular iodine serves as the biocide, while the Russian space agency utilizes silver as a biocide in their systems. In both cases, the biocides must be maintained at a level sufficient to control bacterial growth, but low enough to avoid any negative effects on crew health. For example, the

  18. Colorimetric detection of glucose based on gold nanoparticles coupled with silver nanoparticles (United States)

    Gao, Yan; Wu, Yiting; Di, Junwei


    We have coupled gold nanoparticles (AuNPs) with silver nanoparticles (AgNPs) to assemble a plasmonic sensing platform for colorimetric detection of glucose. In this system, small AuNPs ( 4 nm) can act as glucose oxidase (GOD) mimic enzyme to catalytically oxidize glucose in the presence of oxygen, producing hydrogen peroxide, which dissolves AgNPs to lead the color changes. Glucose can be detected not only by naked eyes (from yellow to red) but also by spectrophotometer in the concentration range of 5-70 μM, with detection limit of 3 μM. More importantly, we found that L-cysteine added in the system can markedly improve the selectivity for the detection of glucose. The proposed method was used to application for the detection of glucose in human serum with satisfactory results. This system is simple and low cost without using any enzymes and organic chromogenic agents.

  19. Ceramics and reflected light new pearlescent and iridescent techniques and colorimetric characterization

    Directory of Open Access Journals (Sweden)

    V. Echarri Iribarren


    Full Text Available Recent years have witnessed the development of innovative applications for ceramic materials as building envelopes. However, little research has been conducted on the relationship between these materials and reflected light, visual perception and integration in the landscape. The aim of the present research was to study the production and application of porcelain stoneware panels in an auditorium. The idea behind using a ceramic coating with an iridescent, pearlescent finish and a metallic sheen was to create a vibrant, constantly changing surface that reflected the natural light and the environment. We designed a metal deposition and glazing process based on three successive firings, and analysed the glaze properties and microcracking factors that reinforced the iridescent lustre. We also performed a colorimetric analysis to assess the goniochromatic or iridescent colours, measuring the spectral radiance of the light.

  20. Determination of pork spoilage by colorimetric gas sensor array based on natural pigments. (United States)

    Huang, Xiao-wei; Zou, Xiao-bo; Shi, Ji-yong; Guo, Yanin; Zhao, Jie-wen; Zhang, Jianchun; Hao, Limin


    A new colorimetric gas-sensor array based on four natural pigments, that were extracted from spinach (Spinacia oleracea), red radish (Raphanus sativus L.), winter jasmine (Jasminum nudiflorum), and black rice (Oryza sativa L. indica), was developed for pork freshness evaluation. A colour change profile for each sample was obtained by differentiating the images of the sensor array before and after exposure to the odour of sample. The total viable count (TVC) per gram of pork was obtained by classical microbiological plating methods, and the biogenic amines were measured by HPLC. Biogenic amine index (BAI) for the determination of meat freshness was developed from the sum of putrescine and cadaverine. The colour change profiles were analysed using principal component analysis and correlated with conventional methods (BAI, TVC). A partial least squares (PLS) prediction model was obtained with r=0.854 and 0.933 for BAI and TVC, respectively.

  1. New Azobenzene Dye Colorimetric and Ratiometric Chemosensors for Mercury(Ⅱ)Ion

    Institute of Scientific and Technical Information of China (English)

    GUO Min; XUE wei; GUAN Mingyun; SUN Jianhua; YIN Gui


    A new series of azobenzene dyes,which possessed colorimetric and ratiometric recognition to Hg2+ based on the mechanism of internal charge transfer(ICT),was developed and characterized.The molecules involving azo and imino functional groups can coordinate with Hg2+ to result in a large blue shift from 453 to 363 nm corresponding to a notable color change from orange to pale yellow in aqueous methanol solution(H2O/CH3OH = 1/4,V/V),which can be applied to naked eye detection of Hg2+.The sensitivity,selectivity and binding mode of the sensors to Hg2+ were investigated by UV-Vis spectroscopy.

  2. "Naked-eye" colorimetric and "turn-on" fluorometric chemosensors for reversible Hg2+ detection. (United States)

    Wanichacheva, Nantanit; Praikaew, Panida; Suwanich, Thanapat; Sukrat, Kanjarat


    Two new Hg(2+)-colorimetric and fluorescent sensors based on 2-[3-(2-aminoethylsulfanyl) propylsulfanyl]ethanamine covalently bound to one and two units of rhodamine-6G moieties, 1 and 2, were synthesised, and their sensing behaviors toward metal ions were investigated by UV/Vis and fluorescence spectroscopy. Upon the addition of Hg(2+), the sensors exhibited highly sensitive "turn-on" fluorescence enhancement as well as a color change from colorless to pink, which was readily noticeable for naked eye detection. Especially, 1 exhibited the reversible behavior and revealed a very high selectivity in the presence of competitive ions, particularly Cu(2+), Ag(+), Pb(2+), Ca(2+), Cd(2+), Co(2+), Fe(2+), Mn(2+), Na(+), Ni(2+), K(+), Ba(2+), Li(+) and Zn(2+), with a low detection limit of 1.7 ppb toward Hg(2+).

  3. SEM-contour-based OPC model calibration through the process window (United States)

    Vasek, Jim; Menedeva, Ovadya; Levitzky, Dan; Lindman, Ofer; Nemadi, Youval; Bailey, George E.; Sturtevant, John L.


    As design rules shrink, there is an unavoidable increase in the complexity of OPC/RET schemes required to enable design printability. These complex OPC/RET schemes have been facilitating unprecedented yield at k I factors previously deemed "unmanufacturable", but they increase the mask complexity and production cost, and can introduce yield-detracting errors. The most common errors are found in OPC design itself, and in the resulting patterning robustness across the process window. Two factors in the OPC design process that contribute to these errors are a) that 2D structures used in the design are not sufficiently well-represented in the OPC model calibration test pattern suite, and b) that the OPC model calibration is done only at the nominal process settings and not across the entire focus-exposure window. This work compares two alternative methods for calibrating OPC models. The first method uses a traditional industry flow for making CD measurements on standard calibration target structures. The second method uses 2D contour profiles extracted automatically by the CD-SEM over varying focus and exposure conditions. OPC models were developed for aggressive quadrupole illumination conditions (k I=0.35) used in 65nm- and 45nm-node logic gate patterning. Model accuracy improvement using 2D contours for calibration through the process window is demonstrated. Additionally this work addresses the issues of automating the contour extraction and calibration process, reducing the data collection burden with improved calibration cycle time.

  4. Comparison of thiaminase activity in fish using the radiometric and 4-nitrothiophenol colorimetric methods (United States)

    Honeyfield, D.C.; Hanes, J.W.; Brown, L.; Kraft, C.E.; Begley, T.P.


    Thiaminase induced thiamine deficiency occurs in fish, humans, livestock and wild animals. A non-radioactive thiaminase assay was described in 2007, but a direct comparison with the radioactive 14C-thiamine method which has been in use for more than 30years has not been reported. The objective was to measure thiaminase activity in forage fish (alewife Alosa pseudoharengus, rainbow smelt Osmerus mordax, and slimy sculpin Cottus cognatus) consumed by predators that manifest thiamine deficiency using both methods. Modifications were made to the colorimetric assay to improve repeatability. Modification included a change in assay pH, enhanced sample clean-up, constant assay temperature (37??C), increase in the concentration of 4-nitrothiophenol (4NTP) and use of a spectrophotometer fitted with a 0.2cm cell. A strong relationship between the two assays was found for 51 alewife (R2=0.85), 36 smelt (R2=0.87) and 20 sculpin (R2=0.82). Thiaminase activity in the colorimetric assay was about 1000 times higher than activity measured by the radioactive method. Application of the assay to fish species from which no thiaminase activity has previously been reported resulted in no 4NTP thiaminase activity being found in bloater Coregonus hoyi, lake trout Salvelinus namaycusch, steelhead trout Oncorhynchus mykiss or Chinook salmon Oncorhynchus tshawytscha. In species previously reported to contain thiaminase, 4NTP thiaminase activity was measured in bacteria Paenibacillus thiaminolyticus, gizzard shad Dorosoma cepedianum, bracken fern Pteridium aquilinum, quagga mussel Dreissena bugensis and zebra mussels D. polymorpha. ?? 2010.


    Directory of Open Access Journals (Sweden)

    Shaza Wageialla Shantier


    Full Text Available This study was designed to develop two colorimetric methods for the determination of Cefquinome Sulphate (CS in bulk and dosage forms using two different concentrations of molybdenum solution. The developed methods were based on the oxidation of CS with 2% ammonium molybdate, in the presence of sulphuric acid, producing a green colored product with λmax at 409 nm (Method I and the oxidation of CS with 10% ammonium molybdate in acidic media to produce a blue colored product with λmax at 673 nm (Method II. The factors affecting the color development and stability were optimized and incorporated in the procedure. Beer’s law was obeyed over the concentration range 16-80 µg mL-1 (Method I and 40-80 µg mL-1 (Method II with a correlation coefficient not less than 0.999. The limit of detection and limit of quantification were 5.7 and 18.9 µg mL-1 for Method I, 4.25 µg mL-1 and 14.2 µg mL-1 for Method II respectively. The average recovery for the dosage form (suspension 2.5% was 100.30% ±0.59; n = 3, which reflected no interference by the suspension excipients. The results obtained by the developed methods for the suspension dosage form were statistically compared with those of a developed HPLC method and evaluated at 95% confidence limits. The developed methods were proved to be accurate and simple. The methods involved in the study covered, Colorimetric spectrophotometry, High Performance Liquid Chromatography. The molar ratio method is recommended to be conducted in order to determine the reaction stoichiometry.

  6. Colorimetric chemosensor of symmetrical benzoylthiourea derivatives as for detection of Cu2+ in aqueous solution (United States)

    Hamedan, N. A.; Hasan, S.; Zaki, H. M.; Alias, N. Z.


    A novel receptor, designed with a combination of oxygen (O), nitrogen (N) and sulfur (S) -binding sites for metal ions was synthesized. Ortho (A), meta (B) and para (C) bearing benzoyl thiourea were designed and synthesized with triamine group to apply as colorimetric chemosensors for detection of Cu2+. The structure was confirmed by characterized the compound using Elemental analysis, Fourier Infrared (FTIR) and proton Nuclear Magnetic Resonance (1H NMR) spectroscopy. Functional groups of C=O, N-H, C=N and C=S were found at 1677 cm-1, 3240 cm-1, 1591 cm-1, 1024 cm-1 respectively while 1H NMR shows peaks of alkane (CH2), benzene (Ar-H), CONH, CSNH at 3.68 – 4.14, 7.16 – 7.86, 8.74, and 9.2 respectively. Elemental analysis for A, B and C C20H21N5O2S2Br2 found was compatible with the expected theoretical calculation. For an application, all of these three sensors showed excellent colorimetric specific selectivity and high sensitivity for Cu2+ in acetonitrile/water binary solutions, so only A was selected for further studies towards sensitivity. When Cu2+ was added to the solution of A, a dramatic color change from yellow to green, while other cations Fe2+, Zn2+, Ni2+, Co2+, Cr3+ and Mn2+ did not interfere with the recognition process for Cu2+. The detection limit of the sensor C toward Cu2+ was 1.15 x 10-5 M, which is less sensitive that sensor A and B with a detection limit of 6.2 x 10-6 M and 1.5 x 10-6 M respectively. This indicated that the sensor A and B might be useful as an efficient chemical sensor.

  7. Antibody modified gold nanoparticles for fast and selective, colorimetric T7 bacteriophage detection. (United States)

    Lesniewski, Adam; Los, Marcin; Jonsson-Niedziółka, Martin; Krajewska, Anna; Szot, Katarzyna; Los, Joanna M; Niedziolka-Jonsson, Joanna


    Herein, we report a colorimetric immunosensor for T7 bacteriophage based on gold nanoparticles modified with covalently bonded anti-T7 antibodies. The new immunosensor allows for a fast, simple, and selective detection of T7 virus. T7 virions form immunological complexes with the antibody modified gold nanoparticles which causes them to aggregate. The aggregation can be observed with the naked eye as a color change from red to purple, as well as with a UV-vis spectrophotometer. The aggregate formation was confirmed with SEM imaging. Sensor selectivity against the M13 bacteriophage was demonstrated. The limit of detection (LOD) is 1.08 × 10(10) PFU/mL (18 pM) T7. The new method was compared with a traditional plaque test. In contrast to biological tests the colorimetric method allows for detection of all T7 phages, not only those biologically active. This includes phage ghosts and fragments of virions. T7 virus has been chosen as a model organism for adenoviruses. The described method has several advantages over the traditional ones. It is much faster than a standard plaque test. It is more robust since no bacteria-virus interactions are utilized in the detection process. Since antibodies are available for a large variety of pathogenic viruses, the described concept is very flexible and can be adapted to detect many different viruses, not only bacteriophages. Contrary to the classical immunoassays, it is a one-step detection method, and no additional amplification, e.g., enzymatic, is needed to read the result.

  8. Irregular-shaped platinum nanoparticles as peroxidase mimics for highly efficient colorimetric immunoassay. (United States)

    Gao, Zhuangqiang; Xu, Mingdi; Hou, Li; Chen, Guonan; Tang, Dianping


    Enzyme-linked immunosorbent assay (ELISA) methods based on natural enzyme-labeled probes have been applied in the immunoassays, but most have some inevitable limitations (e.g. harsh preparation, purification and storage) and are unsuitable for routine use. Herein we synthesized a new class of irregular-shaped platinum nanoparticles (ISPtNP) with a mean length of 7.0 nm and a narrowing width from 2.0 to 5.0 nm along the longitudinal axes, which were utilized as peroxidase-like mimics for the development of colorimetric immunoassays. Compared with bioactive horseradish peroxidase (HRP), the synthesized ISPtNP exhibited a low Km value (~0.12 mM) and a high Kcat value (~2.27×10(4)s(-1)) for 3,3',5,5'-tetramethylbenzidine (TMB) with strong thermal stability and pH tolerance. The catalytic mechanism of the ISPtNP toward TMB/H2O2 was for the first time discussed and deliberated in this work. Based on a sandwich-type assay format, two types of colorimetric immunoassay protocols were designed and developed for the detection of rabbit IgG (RIgG, as a model) by using the synthesized ISPtNP and conventional HRP as the labeling of detection antibodies, respectively. Similar detection limits (LODs) of 2.5 ng mL(-1) vs. 1.0 ng mL(-1) were obtained toward RIgG with the ISPtNP labeling compared to HRP format. Intra- and inter-assay coefficients of variation were less than 13%. Importantly, the ISPtNP-based assay system could be suitable for use in a mass production of miniaturized lab-on-a-chip devices and open new opportunities for protein diagnostics and biosecurity.

  9. Determination of colloidal and dissolved silver in water samples using colorimetric solid-phase extraction. (United States)

    Hill, April A; Lipert, Robert J; Porter, Marc D


    The increase in bacterial resistance to antibiotics has led to resurgence in the use of silver as a biocidal agent in applications ranging from washing machine additives to the drinking water treatment system on the International Space Station (ISS). However, growing concerns about the possible toxicity of colloidal silver to bacteria, aquatic organisms and humans have led to recently issued regulations by the US EPA and FDA regarding the usage of silver. As part of an ongoing project, we have developed a rapid, simple method for determining total silver, both ionic (silver(I)) and colloidal, in 0.1-1mg/L aqueous samples, which spans the ISS potable water target of 0.3-0.5mg/L (total silver) and meets the US EPA limit of 0.1mg/L in drinking water. The method is based on colorimetric solid-phase extraction (C-SPE) and involves the extraction of silver(I) from water samples by passage through a solid-phase membrane impregnated with the colorimetric reagent DMABR (5-[4-(dimethylamino)benzylidene]rhodanine). Silver(I) exhaustively reacts with impregnated DMABR to form a colored compound, which is quantified using a handheld diffuse reflectance spectrophotometer. Total silver is determined by first passing the sample through a cartridge containing Oxone, which exhaustively oxidizes colloidal silver to dissolved silver(I). The method, which takes less than 2 min to complete and requires only approximately 1 mL of sample, has been validated through a series of tests, including a comparison with the ICP-MS analysis of a water sample from ISS that contained both silver(I) and colloidal silver. Potential earth-bound applications are also briefly discussed.



    Bekirova, Lala Rustam; Azerbaijan State Oil Academy, Baku, Azerbaijan


    The possibility of development of two-level RGB colorimetric systems of remote sensing is analyzed. The principle of validation in multi-level RGB colorimetric systems taking into account the effect of metamerizm is formulated Проанализирована возможность построения двухуровневых RGB колориметрических систем дистанционного зондирования. Сформулирован принцип валидации в многоуровневых RGB колориметрических системах с учетом эффекта метамерии Проаналізовано можливість побудови дворівневи...

  11. An integrated approach to monitoring the calibration stability of operational dual-polarization radars (United States)

    Vaccarono, Mattia; Bechini, Renzo; Chandrasekar, Chandra V.; Cremonini, Roberto; Cassardo, Claudio


    monitor the stability of the radar calibration with an accuracy of about 2 dB. This is considered adequate to automatically detect any unexpected change in the radar system requiring further data analysis or on-site measurements.

  12. Reliability-Based Code Calibration

    DEFF Research Database (Denmark)

    Faber, M.H.; Sørensen, John Dalsgaard


    The present paper addresses fundamental concepts of reliability based code calibration. First basic principles of structural reliability theory are introduced and it is shown how the results of FORM based reliability analysis may be related to partial safety factors and characteristic values....... Thereafter the code calibration problem is presented in its principal decision theoretical form and it is discussed how acceptable levels of failure probability (or target reliabilities) may be established. Furthermore suggested values for acceptable annual failure probabilities are given for ultimate...... and serviceability limit states. Finally the paper describes the Joint Committee on Structural Safety (JCSS) recommended procedure - CodeCal - for the practical implementation of reliability based code calibration of LRFD based design codes....

  13. Reduced Ambiguity Calibration for LOFAR

    CERN Document Server

    Yatawatta, Sarod


    Interferometric calibration always yields non unique solutions. It is therefore essential to remove these ambiguities before the solutions could be used in any further modeling of the sky, the instrument or propagation effects such as the ionosphere. We present a method for LOFAR calibration which does not yield a unitary ambiguity, especially under ionospheric distortions. We also present exact ambiguities we get in our solutions, in closed form. Casting this as an optimization problem, we also present conditions for this approach to work. The proposed method enables us to use the solutions obtained via calibration for further modeling of instrumental and propagation effects. We provide extensive simulation results on the performance of our method. Moreover, we also give cases where due to degeneracy, this method fails to perform as expected and in such cases, we suggest exploiting diversity in time, space and frequency.

  14. Astrid-2 EMMA Magnetic Calibration

    DEFF Research Database (Denmark)

    Merayo, José M.G.; Brauer, Peter; Risbo, Torben


    The Swedish micro-satellite Astrid-2 contains a tri-axial fluxgate magnetometer with the sensor co-located with a Technical University of Denmark (DTU) star camera for absolute attitude, and extended about 0.9 m on a hinged boom. The magnetometer is part of the RIT EMMA electric and magnetic fields...... Survey of Sweden near Stockholm on the night of May 15.-16., 1997. The magnetic calibration and the intercalibration between the star camera and the magnetic sensor was performed by measuring the Earth's magnetic field and simultaneously observing the star sky with the camera. The rotation matrix between...... fit calibration parameters. Owing to time shortage, we did not evaluate the temperature coefficients of the flight sensor calibration parameters. However, this was done for an identical flight spare magnetometer sensor at the magnetic coil facility belonging to the Technical University of Braunschweig...

  15. On chromatic and geometrical calibration

    DEFF Research Database (Denmark)

    Folm-Hansen, Jørgen


    of non-uniformity of the illumination of the image plane. Only the image deforming aberrations and the non-uniformity of illumination are included in the calibration models. The topics of the pinhole camera model and the extension to the Direct Linear Transform (DLT) are described. It is shown how......The main subject of the present thesis is different methods for the geometrical and chromatic calibration of cameras in various environments. For the monochromatic issues of the calibration we present the acquisition of monochrome images, the classic monochrome aberrations and the various sources...... the DLT can be extended with non-linear models of the common lens aberrations/errors some of them caused by manufacturing defects like decentering and thin prism distortion. The relation between a warping and the non-linear defects are shown. The issue of making a good resampling of an image by using...

  16. Flexible calibration procedure for fringe projection profilometry


    Vargas, Javier; Quiroga Mellado, Juan Antonio; Terrón López, María José


    A novel calibration method for whole field three-dimensional shape measurement by means of fringe projection is presented. Standard calibration techniques, polynomial-and model-based, have practical limitations such as the difficulty of measuring large fields of view, the need to use precise z stages, and bad calibration results due to inaccurate calibration points. The proposed calibration procedure is a mixture of the two main standard techniques, sharing their benefits and avoiding their m...

  17. An Automatic Proof of Euler's Formula

    Directory of Open Access Journals (Sweden)

    Jun Zhang


    Full Text Available In this information age, everything is digitalized. The encoding of functions and the automatic proof of functions are important. This paper will discuss the automatic calculation for Taylor expansion coefficients, as an example, it can be applied to prove Euler's formula automatically.

  18. Self-Compassion and Automatic Thoughts (United States)

    Akin, Ahmet


    The aim of this research is to examine the relationships between self-compassion and automatic thoughts. Participants were 299 university students. In this study, the Self-compassion Scale and the Automatic Thoughts Questionnaire were used. The relationships between self-compassion and automatic thoughts were examined using correlation analysis…

  19. Automatic Control System for Neutron Laboratory Safety

    Institute of Scientific and Technical Information of China (English)

    ZHAO; Xiao; ZHANG; Guo-guang; FENG; Shu-qiang; SU; Dan; YANG; Guo-zhao; ZHANG; Shuai


    In order to cooperate with the experiment of neutron generator,and realize the automatic control in the experiment,a set of automatic control system for the safety of the neutron laboratory is designed.The system block diagram is shown as Fig.1.Automatic control device is for processing switch signal,so PLC is selected as the core component

  20. Automatic Rectification of Building FAÇADES (United States)

    Tsironis, V.; Tranou, A.; Vythoulkas, A.; Psalta, A.; Petsa, E.; Karras, G.


    Focusing mainly on the case of (near-)planar building façades, a methodology for their automatic projective rectification is described and evaluated. It relies on a suitably configured, calibrated stereo pair of an object expected to contain a minimum of vertical and/or horizontal lines for the purposes of levelling. The SURF operator has been used for extracting and matching interest points. The coplanar points have been separated with two alternative methods. First, the fundamental matrix of the stereo pair, computed using robust estimation, allows estimating the relative orientation of the calibrated pair; initial parameter values, if needed, may be estimated via the essential matrix. Intersection of valid points creates a 3D point set in model space, to which a plane is robustly fitted. Second, all initial point matches are directly used for robustly estimating the inter-image homography of the pair, thus directly selecting all image matches referring to coplanar points; initial values for the relative orientation parameters, if needed, may be estimated from a decomposition of the inter-image homography. Finally, all intersected coplanar model points yield the object-to-image homography to allow image rectification. The in-plane rotation required to finalize the transformation is found by assuming that rectified images contain sufficient straight linear segments to form a dominant pair of orthogonal directions which correspond to horizontality/verticality in 3D space. In our implementation, image edges from Canny detector are used in linear Hough Transform (HT) resulting in a 2D array (ρ, θ) with values equal to the sum of pixels belonging to the particular line. Quantization parameter values aim at absorbing possible slight deviations from collinearity due to thinning or uncorrected lens distortions. By first imposing a threshold expressing the minimum acceptable number of edge-characterized pixels, the resulting HT is accumulated along the ρ-dimension to

  1. Hydrological model calibration for enhancing global flood forecast skill (United States)

    Hirpa, Feyera A.; Beck, Hylke E.; Salamon, Peter; Thielen-del Pozo, Jutta


    Early warning systems play a key role in flood risk reduction, and their effectiveness is directly linked to streamflow forecast skill. The skill of a streamflow forecast is affected by several factors; among them are (i) model errors due to incomplete representation of physical processes and inaccurate parameterization, (ii) uncertainty in the model initial conditions, and (iii) errors in the meteorological forcing. In macro scale (continental or global) modeling, it is a common practice to use a priori parameter estimates over large river basins or wider regions, resulting in suboptimal streamflow estimations. The aim of this work is to improve flood forecast skill of the Global Flood Awareness System (GloFAS;, a grid-based forecasting system that produces flood forecast unto 30 days lead, through calibration of the distributed hydrological model parameters. We use a combination of in-situ and satellite-based streamflow data for automatic calibration using a multi-objective genetic algorithm. We will present the calibrated global parameter maps and report the forecast skill improvements achieved. Furthermore, we discuss current challenges and future opportunities with regard to global-scale early flood warning systems.

  2. JIS B 9921 (light scattering automatic counter). JIS B 9921 (hikari sanranshiki jido ryushi keisuki) ni tsuite

    Energy Technology Data Exchange (ETDEWEB)

    Fujii, S. (Japan Air Cleaning Association, Tokyo (Japan))


    JIS B 9921 (Light scattering automatic counter) enacted in 1976 was revised in November 1989. With the improvement of air cleaning technologies such as clean room, the measurement of airborne particles has become more important. The most convenient and precise measuring instrument is a light scattering particle counter. However there was an error in accuracy between each counter and there were some problems about reliability of its measured values. This was because its calibration method had not been established. Under the circumstances, the sub-micron particle measuring and removing committee was formed in Japan Air Cleaning Association in order to study general rules of calibration, and the results of the study were standardized as Japan Air Cleaning Association Standard, JACA NO. 18 (General rules of calibration for light scattering automatic particle counter). The purpose of the revision this time is to improve the reliability by adopting the above standard. 12 figs., 1 tab.

  3. Tank calibration; Arqueacao de tanques

    Energy Technology Data Exchange (ETDEWEB)

    Chan, Ana [PETROBRAS S.A., Rio de Janeiro, RJ (Brazil)


    This work relates the analysis of the norms ISO (International Organization for Standardization) for calibration of vertical cylindrical tanks used in fiscal measurement, established on Joint Regulation no 1 of June 19, 2000 between the ANP (National Agency of Petroleum) and the INMETRO (National Institute of Metrology, Normalization and Industrial Quality). In this work a comparison between norms ISO and norms published by the API (American Petroleum Institute) and the IP (Institute of Petroleum) up to 2001 was made. It was concluded that norms ISO are wider than norms API, IP, and INMETRO methods in the calibration of vertical cylindrical tanks. (author)

  4. Instrument Calibration and Certification Procedure

    Energy Technology Data Exchange (ETDEWEB)

    Davis, R. Wesley [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)


    The Amptec 640SL-2 is a 4-wire Kelvin failsafe resistance meter, designed to reliably use very low-test currents for its resistance measurements. The 640SL-1 is a 2-wire version, designed to support customers using the Reynolds Industries type 311 connector. For both versions, a passive (analog) dual function DC Milliameter/Voltmeter allows the user to verify the actual 640SL output current level and the open circuit voltage on the test leads. This procedure includes tests of essential performance parameters. Any malfunction noticed during calibration, whether specifically tested for or not, shall be corrected before calibration continues or is completed.

  5. Performance standard for dose Calibrator

    CERN Document Server

    Darmawati, S


    Dose calibrator is an instrument used in hospitals to determine the activity of radionuclide for nuclear medicine purposes. International Electrotechnical Commission (IEC) has published IEC 1303:1994 standard that can be used as guidance to test the performance of the instrument. This paper briefly describes content of the document,as well as explains the assessment that had been carried out to test the instrument accuracy in Indonesia through intercomparison measurement.Its is suggested that hospitals acquire a medical physicist to perform the test for its dose calibrator. The need for performance standard in the form of Indonesia Standard is also touched.

  6. Automatic Schema Evolution in Root

    Institute of Scientific and Technical Information of China (English)

    ReneBrun; FonsRademakers


    ROOT version 3(spring 2001) supports automatic class schema evolution.In addition this version also produces files that are self-describing.This is achieved by storing in each file a record with the description of all the persistent classes in the file.Being self-describing guarantees that a file can always be read later,its structure browsed and objects inspected.also when the library with the compiled code of these classes is missing The schema evolution mechanism supports the frequent case when multiple data sets generated with many different class versions must be analyzed in the same session.ROOT supports the automatic generation of C++ code describing the data objects in a file.

  7. Automatic spikes detection in seismogram

    Institute of Scientific and Technical Information of China (English)

    王海军; 靳平; 刘贵忠


    @@ Data processing for seismic network is very complex and fussy, because a lot of data is recorded in seismic network every day, which make it impossible to process these data all by manual work. Therefore, seismic data should be processed automatically to produce a initial results about events detection and location. Afterwards, these results are reviewed and modified by analyst. In automatic processing data quality checking is important. There are three main problem data thatexist in real seismic records, which include: spike, repeated data and dropouts. Spike is defined as isolated large amplitude point; the other two problem datahave the same features that amplitude of sample points are uniform in a interval. In data quality checking, the first step is to detect and statistic problem data in a data segment, if percent of problem data exceed a threshold, then the whole data segment is masked and not be processed in the later process.

  8. Physics of Automatic Target Recognition

    CERN Document Server

    Sadjadi, Firooz


    Physics of Automatic Target Recognition addresses the fundamental physical bases of sensing, and information extraction in the state-of-the art automatic target recognition field. It explores both passive and active multispectral sensing, polarimetric diversity, complex signature exploitation, sensor and processing adaptation, transformation of electromagnetic and acoustic waves in their interactions with targets, background clutter, transmission media, and sensing elements. The general inverse scattering, and advanced signal processing techniques and scientific evaluation methodologies being used in this multi disciplinary field will be part of this exposition. The issues of modeling of target signatures in various spectral modalities, LADAR, IR, SAR, high resolution radar, acoustic, seismic, visible, hyperspectral, in diverse geometric aspects will be addressed. The methods for signal processing and classification will cover concepts such as sensor adaptive and artificial neural networks, time reversal filt...

  9. Automatic design of magazine covers (United States)

    Jahanian, Ali; Liu, Jerry; Tretter, Daniel R.; Lin, Qian; Damera-Venkata, Niranjan; O'Brien-Strain, Eamonn; Lee, Seungyon; Fan, Jian; Allebach, Jan P.


    In this paper, we propose a system for automatic design of magazine covers that quantifies a number of concepts from art and aesthetics. Our solution to automatic design of this type of media has been shaped by input from professional designers, magazine art directors and editorial boards, and journalists. Consequently, a number of principles in design and rules in designing magazine covers are delineated. Several techniques are derived and employed in order to quantify and implement these principles and rules in the format of a software framework. At this stage, our framework divides the task of design into three main modules: layout of magazine cover elements, choice of color for masthead and cover lines, and typography of cover lines. Feedback from professional designers on our designs suggests that our results are congruent with their intuition.

  10. The Automatic Measurement of Targets

    DEFF Research Database (Denmark)

    Höhle, Joachim


    The automatic measurement of targets is demonstrated by means of a theoretical example and by an interactive measuring program for real imagery from a réseau camera. The used strategy is a combination of two methods: the maximum correlation coefficient and the correlation in the subpixel range. F...... interactive software is also part of a computer-assisted learning program on digital photogrammetry....

  11. Automatically-Programed Machine Tools (United States)

    Purves, L.; Clerman, N.


    Software produces cutter location files for numerically-controlled machine tools. APT, acronym for Automatically Programed Tools, is among most widely used software systems for computerized machine tools. APT developed for explicit purpose of providing effective software system for programing NC machine tools. APT system includes specification of APT programing language and language processor, which executes APT statements and generates NC machine-tool motions specified by APT statements.

  12. Annual review in automatic programming

    CERN Document Server

    Halpern, Mark I; Bolliet, Louis


    Computer Science and Technology and their Application is an eight-chapter book that first presents a tutorial on database organization. Subsequent chapters describe the general concepts of Simula 67 programming language; incremental compilation and conversational interpretation; dynamic syntax; the ALGOL 68. Other chapters discuss the general purpose conversational system for graphical programming and automatic theorem proving based on resolution. A survey of extensible programming language is also shown.

  13. How CBO Estimates Automatic Stabilizers (United States)


    of wages and salaries and proprietors’ incomes as recorded in the NIPAs to changes in the GDP gap , CBO uses separate regressions based on equation (1...Outlays Without Automatic Stabilizers GDP Gapa Unemployment Gap (Percent)b Revenues Outlays 3 Table 1. (Continued) Deficit or Surplus With and...gross domestic product; * = between -0.05 percent and 0.05 percent. a. The GDP gap equals the difference between actual or projected GDP and CBO’s

  14. Automatic translation among spoken languages (United States)

    Walter, Sharon M.; Costigan, Kelly


    The Machine Aided Voice Translation (MAVT) system was developed in response to the shortage of experienced military field interrogators with both foreign language proficiency and interrogation skills. Combining speech recognition, machine translation, and speech generation technologies, the MAVT accepts an interrogator's spoken English question and translates it into spoken Spanish. The spoken Spanish response of the potential informant can then be translated into spoken English. Potential military and civilian applications for automatic spoken language translation technology are discussed in this paper.

  15. The Automatic Galaxy Collision Software

    CERN Document Server

    Smith, Beverly J; Pfeiffer, Phillip; Perkins, Sam; Barkanic, Jason; Fritts, Steve; Southerland, Derek; Manchikalapudi, Dinikar; Baker, Matt; Luckey, John; Franklin, Coral; Moffett, Amanda; Struck, Curtis


    The key to understanding the physical processes that occur during galaxy interactions is dynamical modeling, and especially the detailed matching of numerical models to specific systems. To make modeling interacting galaxies more efficient, we have constructed the `Automatic Galaxy Collision' (AGC) code, which requires less human intervention in finding good matches to data. We present some preliminary results from this code for the well-studied system Arp 284 (NGC 7714/5), and address questions of uniqueness of solutions.

  16. Automatic computation of transfer functions (United States)

    Atcitty, Stanley; Watson, Luke Dale


    Technologies pertaining to the automatic computation of transfer functions for a physical system are described herein. The physical system is one of an electrical system, a mechanical system, an electromechanical system, an electrochemical system, or an electromagnetic system. A netlist in the form of a matrix comprises data that is indicative of elements in the physical system, values for the elements in the physical system, and structure of the physical system. Transfer functions for the physical system are computed based upon the netlist.

  17. Experimental Investigations of a Precision Sensor for an Automatic Weapons Stabilizer System. (United States)

    Korobiichuk, Igor


    This paper presents the results of experimental investigations of a precision sensor for an automatic weapons stabilizer system. It also describes the experimental equipment used and the structure of the developed sensor. A weapons stabilizer is designed for automatic guidance of an armament unit in the horizontal and vertical planes when firing at ground and air targets that are quickly maneuvering, and at lower speeds when firing anti-tank missiles, as well as the bypass of construction elements by the armament unit, and the automatic tracking of moving targets when interacting with a fire control system. The results of experimental investigations have shown that the error of the precision sensor developed on the basis of a piezoelectric element is 6 × 10(-10) m/s² under quasi-static conditions, and ~10(-5) m/s² for mobile use. This paper defines metrological and calibration properties of the developed sensor.

  18. Comparison of inductively coupled plasma mass spectrometry and colorimetric determination of total and extractable phosphorus in soils

    Energy Technology Data Exchange (ETDEWEB)

    Ivanov, Krasimir, E-mail: [Department of Chemistry, University of Agriculture, Plovdiv (Bulgaria); Zaprjanova, Penka [Tobacco and Tobacco Products Institute, Plovdiv (Bulgaria); Petkova, Milena [Department of Chemistry, University of Agriculture, Plovdiv (Bulgaria); Stefanova, Violeta; Kmetov, Veselin; Georgieva, Deyana [Department of Analytical Chemistry, Plovdiv University ' Paisii Hilendarski,' Plovdiv (Bulgaria); Angelova, Violina [Department of Chemistry, University of Agriculture, Plovdiv (Bulgaria)


    The most widely used method for determination of total phosphorus in soils is perchloric acid digestion, followed by a colorimetric assay to measure the concentration of P in solution. The first part of this study compares an alternative digestion method, using aqua regia (ISO 11466 and EPA Method 3052), with perchloric acid digestion procedure, and also compares inductively coupled plasma mass spectroscopy (ICP-MS) with colorimetry for the measurement of P on the basis of five internationally certified standard soils and 20 real-life soils with widely different extractability of phosphorus. The phosphorus concentration was determined by means of the reduced phosphomolybdenum blue and ICP-MS. The relationship between methods has been examined statistically. Good agreement of the results from colorimetry and ICP-MS was established for all certified soils. The microwave-assisted digestion with aqua regia was comparable, both in precision and accuracy, with the hot plate aqua regia method. The phosphorus concentration found with the HF + HClO{sub 4} digestion method was in good agreement with the certified mean values, while the superiority in extracting phosphorus, when compared to other methods, was obvious. Soil testing for plant-available phosphorus in Bulgaria and many European countries is most commonly conducted using acid Ca-lactate extraction (Egner-Riehm test) and alkaline sodium bicarbonate extraction (BDS ISO 11263:2002), based on Olsen test, followed by a colorimetric assay to measure the concentration of P in solution. The second part of this study reports the differences between Egner-Riehm test and BDS ISO 11263:2002 measured colorimetrically and by ICP-MS. Fifty soils were selected from South Bulgaria to represent a wide range of soil properties. It was established that ICP-MS consistently yielded significantly higher P concentrations than the colorimetric method in both extraction tests, and the relative differences were greatest in soils with lower

  19. Discrimination of Chinese green tea according to varieties and grade levels using artificial nose and tongue based on colorimetric sensor arrays. (United States)

    Huo, Danqun; Wu, Yu; Yang, Mei; Fa, Huanbao; Luo, Xiaogang; Hou, Changjun


    Colorimetric artificial tongue and nose were used to discriminate nine Chinese green teas from different geographical origins and grade levels. Printing nanoporous porphyrin, dimeric metalloporphyrins, metallosalophen complexes and chemically responsive dyes on a hydrophobic membrane, the developed sensor array of artificial tongue and nose showed a unique pattern of colorimetric change upon exposure to green tea liquids or gases. All green tea samples, both in liquid and gas analysis, gave distinct patterns according to geographical origin and grade level, thus resulting in their facile identification. The good reproducibility of colorimetric artificial tongue and nose was proved. Data analysis was performed by chemometric techniques: hierarchical cluster analysis (HCA), and principal component analysis (PCA). Chinese green tea from the same geographical origin could cluster together in PCA score plot. No errors in classification by HCA were observed in 90 trials. The colorimetric artificial tongue and nose can be used to discriminate Chinese green tea according to geographical origin and grade level.

  20. Long Baseline Stereovision for Automatic Detection and Ranging of Moving Objects in the Night Sky

    Directory of Open Access Journals (Sweden)

    Vlad Turcu


    Full Text Available As the number of objects in Earth’s atmosphere and in low Earth orbit is continuously increasing; accurate surveillance of these objects has become important. This paper presents a generic, low cost sky surveillance system based on stereovision. Two cameras are placed 37 km apart and synchronized by a GPS-controlled external signal. The intrinsic camera parameters are calibrated before setup in the observation position, the translation vectors are determined from the GPS coordinates and the rotation matrices are continuously estimated using an original automatic calibration methodology based on following known stars. The moving objects in the sky are recognized as line segments in the long exposure images, using an automatic detection and classification algorithm based on image processing. The stereo correspondence is based on the epipolar geometry and is performed automatically using the image detection results. The resulting experimental system is able to automatically detect moving objects such as planes, meteors and Low Earth Orbit satellites, and measure their 3D position in an Earth-bound coordinate system.