WorldWideScience

Sample records for automated mass action

  1. Automated transportable mass spectrometer

    International Nuclear Information System (INIS)

    The need has been identified for a Mass Spectrometer (MS) which can be conveniently transported among several facilities for rapid verification of the isotopic composition of Special Nuclear Material. This requirement for a light weight, transportable MS for U and Pu mass analysis was met by deleting the gas chromatograph (GC) portions of a Hewlett-Packard (H-P) Model 5992 Quadrupole GCMS and substituting a vacuum lock sample entry system. A programmable power supply and vacuum gauge were added and circuitry modifications were made to enable use of the supplied software. The single rhenium filament plug-in source is loaded with either a nominal microliter of sample solution and evaporated, or with a prepared resin bead. Using a resin bead in a specially dimpled filament, copious sensitivity is obtained with 30 nanogram uranium samples. After sample insertion the analysis is completely controlled by an H-P Model 9825 calculator. Source vacuum of 2 x 10-7 torr or better is regained within 2 minutes after sample insertion, and total time for a complete analysis is about 7 minutes. Accuracy is better than 1% for isotope ratios less than 20 and better than 2% for ratios of 100. Ions are accelerated at about 1.8 volts into the mass filter which has pole pieces of hyperbolic cross section. Collection is by a Galileo Channeltron multiplier into a log preamp. During a normal run, 1.4 x 106 data point are observed and averaged. Weight of the instrument excluding the calculator is 88 Kg which allows relatively easy transportation over short distances by two persons. The instrument can be carried into a facility and be ready to analyze samples in less than 3 hours

  2. Freeman's mass action

    OpenAIRE

    Freeman, Walter J III; Kozma, Robert

    2010-01-01

    Freeman's Mass Action (FMA) refers to the collective synaptic actions that neurons in the cortex exert on each other in vast numbers by synchronizing their firing of action potentials. In the aggregate, FMA is a powerful force that creates bursts of cortical neural activity that resemble the vortices of tornadoes and hurricanes. The bursts rapidly and repeatedly retrieve memories and bind them with sensory information into percepts. In this way, FMA expresses and transmits the meaning of sens...

  3. Comments on the balance between automation and human actions

    International Nuclear Information System (INIS)

    During the past two or three decades many industrial plants have become more and more large-scale and complex, which was possible by the enhancement of automation of their operation. However, very rapid increase in the scale and complexity of the plants sometimes caused accidents; sometimes by failure of automatic system hardware or software and sometimes by the operator's error. Since the status and general trends in Japan related to this problem has already been reported in the previous meeting on the subject, the paper gives very briefly some general comments on this problem based on the practical experience with the development of a computerized control system for a large-scale test facility constructed at the Japan Atomic Energy Research Institute (JAERI) in which the Reactor Control Laboratory has participated with special attention to its man-machine interface design. The balance between the automation and human action in the operation of complex plants depends on various factors such as technological, economical, ergonomical and societal factors which are more or less mutually interrelated and may vary with time. Since hardwares of the automated system may fail and operators may make errors, the level of automation should be determined by taking all these factors into consideration so that it may be welcome by the operators as well as by the society. In this connection, more research must be made to investigate an appropriate guideline for determining the proper level of automation of complex plants having large potential risks. (author). 3 refs, 2 figs

  4. A portable, automated, inexpensive mass and balance calibration system

    International Nuclear Information System (INIS)

    Reliable mass measurements are essential for a nuclear production facility or process control laboratory. DOE Order 5630.2 requires that traceable standards be used to calibrate and monitor equipment used for nuclear material measurements. To ensure the reliability of mass measurements and to comply with DOE traceability requirements, a portable, automated mass and balance calibration system is used at the Savannah River Plant. Automation is achieved using an EPSON HX-20 notebook computer, which can be operated via RS232C interfacing to electronic balances or function with manual data entry if computer interfacing is not feasible. This economical, comprehensive, user-friendly system has three main functions in a mass measurement control program (MMCP): balance certification, calibration of mass standards, and daily measurement of traceable standards. The balance certification program tests for accuracy, precision, sensitivity, linearity, and cornerloading versus specific requirements. The mass calibration program allows rapid calibration of inexpensive mass standards traceable to certified Class S standards. This MMCP permits daily measurement of traceable standards to monitor the reliability of balances during routine use. The automated system verifies balance calibration, stores results for future use, and provides a printed control chart of the stored data. Another feature of the system permits three different weighing routines that accommodate their need for varying degrees of reliability in routine weighing operations

  5. Automation of a thermal ionisation mass spectrometer

    International Nuclear Information System (INIS)

    A thermal ionization mass spectrometer was upgraded in order to be monitored by a PC. A PC-LMP-16 National Instruments data acquisition board was used for the ion current channel and the Hall signal channel. A dedicated interface was built to allow commands from the computer to the current supply of the analyzing magnet and to the high voltage unit of the mass spectrometer. A software application was worked out to perform the adjustment of the spectrometer, magnetic scanning and mass spectra acquisition, data processing and isotope ratio determination. The apparatus is used for isotope ratio 235 U/238 U determination near the natural abundance. A peak jumping technique is applied to choose between the 235 U and 238 U signal, by switching the high voltage applied to the ion source between two preset values. This avoids the delay between the acquisition of the peaks of interest, a delay that would appear in the case of a 'pure' magnetic scanning. Corrections are applied for the mass discrimination effects and a statistical treatment of the data is achieved. (authors)

  6. Automated data processing of high-resolution mass spectra

    DEFF Research Database (Denmark)

    Hansen, Michael Adsetts Edberg; Smedsgaard, Jørn

    infusion of crude extracts into the source taking advantage of the high sensitivity, high mass resolution and accuracy and the limited fragmentation. Unfortunately, there has not been a comparable development in the data processing techniques to fully exploit gain in high resolution and accuracy of the...... massive amounts of data. We present an automated data processing method to quantitatively compare large numbers of spectra from the analysis of complex mixtures, exploiting the full quality of high-resolution mass spectra. By projecting all detected ions - within defined intervals on both the time and...... mass axis on to a fixed one-dimensional array, we obtain a vector that can be used directly as input in multivariate statistics or library search methods. We demonstrate that both cluster- and discriminant analysis as well as PCA (and related methods) can be applied directly on mass spectra from direct...

  7. Balancing automation and human action in nuclear power plants

    International Nuclear Information System (INIS)

    The 130 participants, representing 28 Member States and 3 international organizations, reviewed recent developments and discussed directions for future efforts in the area of balancing automation and human control in NPPs. During the symposium, 41 papers and 12 posters were presented covering the wide spectrum of activities in the area. In recent years very rapid progress in the development of automation technology has produced drastic reductions in the cost of equipment. Care has to be taken to establish a reasonable balance in NPP design and operation between automation and human control so as to ensure both maximum safety and availability. Balancing problem was discussed for all tasks, i.e. operation, maintenance and testing, including all levels from information processing to full automation. Validation, verification and training aspects were specifically considered. The Proceedings of the Conference is divided into 8 sessions: invited papers (3 papers); conceptions and achievements in automation (7 papers); safety implication of automation (6 papers); computerized operator support, part 1 (5 papers), part 2 (6 papers); man-machine interaction (9 papers); human factors consideration in automation (9 papers); accident management in automation (8 papers). A separate abstract was prepared for each of these papers. Refs, figs and tabs

  8. Automated Determination of a Package's Center of Mass

    Directory of Open Access Journals (Sweden)

    Ayaz Hemani

    2010-01-01

    Full Text Available In order to address the issue of increased efficiency and better planning for parcel shipments, an automated computer program was developed in Microsoft Excel that calculates center of mass and moments of mass with greater speed and reliability than currently implemented systems. This simple program requires only a variable density function and limits of integration for a given object as input within the spreadsheet system. Once the required input has been provided, a series of chain calculations, with the help of a Visual Basic for Applications (VBA script, is able to process the input, which is done through integration and a Riemann sum. Furthermore, the foundation of the program can also be used for calculating other physical quantities of interest such as the moment of inertia or surface area of an object.

  9. Automated spike preparation system for Isotope Dilution Mass Spectrometry (IDMS)

    International Nuclear Information System (INIS)

    Isotope Dilution Mass Spectrometry (IDMS) is a method frequently employed to measure dissolved, irradiated nuclear materials. A known quantity of a unique isotope of the element to be measured (referred to as the ''spike'') is added to the solution containing the analyte. The resulting solution is chemically purified then analyzed by mass spectrometry. By measuring the magnitude of the response for each isotope and the response for the ''unique spike'' then relating this to the known quantity of the ''spike'', the quantity of the nuclear material can be determined. An automated spike preparation system was developed at the Savannah River Site (SRS) to dispense spikes for use in IDMS analytical methods. Prior to this development, technicians weighed each individual spike manually to achieve the accuracy required. This procedure was time-consuming and subjected the master stock solution to evaporation. The new system employs a high precision SMI Model 300 Unipump dispenser interfaced with an electronic balance and a portable Epson HX-20 notebook computer to automate spike preparation

  10. Automating ActionScript Projects with Eclipse and Ant

    CERN Document Server

    Koning, Sidney

    2011-01-01

    Automating repetitive programming tasks is easier than many Flash/AS3 developers think. With the Ant build tool, the Eclipse IDE, and this concise guide, you can set up your own "ultimate development machine" to code, compile, debug, and deploy projects faster. You'll also get started with versioning systems, such as Subversion and Git. Create a consistent workflow for multiple machines, or even complete departments, with the help of extensive Ant code samples. If you want to work smarter and take your skills to a new level, this book will get you on the road to automation-with Ant. Set up y

  11. Hypervascular mediastinal masses: Action points for radiologists

    International Nuclear Information System (INIS)

    Highlights: •An algorithm combining clinical data and radiology features of hypervascular mediastinal masses is proposed to determine further evaluation and subsequently guide treatment. •Characteristic features and known association with syndromes and genetic mutations assists in achieving a diagnosis. •MRI and functional imaging can be very helpful in the evaluation of hypervascular mediastinal masses. •Identification of hypervascularity within mediastinal masses should alert the radiologist and clinician and an attempt should be made to preferably avoid percutaneous CT guided biopsies and attempt tissue sampling surgically with better control of post procedure hemorrhage. -- Abstract: Hypervascular mediastinal masses are a distinct group of rare diseases that include a subset of benign and malignant entities. Characteristic features and known association with syndromes and genetic mutations assist in achieving a diagnosis. Imaging allows an understanding of the vascularity of the lesion and should alert the radiologist and clinician to potential hemorrhagic complications and avoid percutaneous CT guided biopsies. In such cases, pre-procedure embolization and surgical biopsy maybe considered for better control of post procedure hemorrhage. The purpose of this article is to describe and illustrate the clinical features and radiologic spectrum of hypervascular mediastinal masses, and discuss the associated clinical and genetic syndromes. We will present an imaging algorithm to determine further evaluation and subsequently guide treatment

  12. Mass Action Dynamics of Coupled Reactions using Fluctuation Theory

    OpenAIRE

    Cannon, William R.; Baker, Scott E.

    2015-01-01

    Comprehensive and predictive simulation of coupled reaction networks has long been a goal of biology and other fields. Currently, metabolic network models that utilize enzyme mass action kinetics have predictive power but are limited in scope and application by the fact that the determination of enzyme rate constants is laborious and low throughput. We present a statistical thermodynamic formulation of the law of mass action for coupled reactions at both steady states and non-stationary state...

  13. Balance between automation and human actions in nuclear power plant operation. Results of international cooperation

    International Nuclear Information System (INIS)

    Automation has long been an established feature of power plants. In some applications, the use of automation has been the significant factor which has enabled plant technology to progress to its current state. Societal demands for increased levels of safety have led to greater use of redundancy and diversity and this, in turn, has increased levels of automation. However, possibly the greatest contributory factor in increased automation has resulted from improvements in information technology. Much recent attention has been focused on the concept of inherently safe reactors, which may simplify safety system requirements and information and control system complexity. The allocation of tasks between man and machine may be one of the most critical activity in the design of new nuclear plants and major retro-fits and it therefore warrants a design approach which is commensurate in quality with the high levels of safety and production performance sought from nuclear plants. Facing this climate, in 1989 the International Atomic Energy Agency (IAEA) formed an advisory group from member countries with extensive experience in nuclear power plant automation. The task of this group was to advise on the appropriate balance between manual and automatic actions in plant operation. (author)

  14. Balance between automation and human actions in nuclear power plant operation. Results of international cooperation

    International Nuclear Information System (INIS)

    Automation has long been an established feature of power plants. In some applications, the use of automation has been the significant factor which has enabled plant technology to progress to its current state. Societal demands for increased levels of safety have led to greater use of redundancy and diversity and this, in turn, has increased levels of automation. However, possibly the greatest contributory factor in increased automation has resulted from improvements in information technology. Much recent attention has been focused on the concept of inherently safe reactors, which may simplify safety system requirements and information and control system complexity. The allocation of tasks between man and machine may be one of the most critical activity in the design of new nuclear plants and major retro-fits and it therefore warrants a design approach which is commensurate in quality with the high levels of safety and production performance sought from nuclear plants. Facing this climate, in 1989 the International Atomic Energy Agency (IAEA) formed an advisory group from member countries with extensive experience in nuclear power plant automation. The task of this group was to advise on the appropriate balance between manual and automatic actions in plant operation

  15. Open complex-balanced mass action chemical reaction networks

    NARCIS (Netherlands)

    Rao, Shodhan; van der Schaft, Arjan; Jayawardhana, Bayu

    2014-01-01

    We consider open chemical reaction networks, i.e. ones with inflows and outflows. We assume that all the inflows to the network are constant and all outflows obey the mass action kinetics rate law. We define a complex-balanced open reaction network as one that admits a complex-balanced steady state.

  16. Automated reduction and interpretation of multidimensional mass spectra for analysis of complex peptide mixtures

    Science.gov (United States)

    Gambin, Anna; Dutkowski, Janusz; Karczmarski, Jakub; Kluge, Boguslaw; Kowalczyk, Krzysztof; Ostrowski, Jerzy; Poznanski, Jaroslaw; Tiuryn, Jerzy; Bakun, Magda; Dadlez, Michal

    2007-01-01

    Here we develop a fully automated procedure for the analysis of liquid chromatography-mass spectrometry (LC-MS) datasets collected during the analysis of complex peptide mixtures. We present the underlying algorithm and outcomes of several experiments justifying its applicability. The novelty of our approach is to exploit the multidimensional character of the datasets. It is common knowledge that highly complex peptide mixtures can be analyzed by liquid chromatography coupled with mass spectrometry, but we are not aware of any existing automated MS spectra interpretation procedure designed to take into account the multidimensional character of the data. Our work fills this gap by providing an effective algorithm for this task, allowing for automated conversion of raw data to the list of masses of peptides.

  17. Variable elimination in chemical reaction networks with mass action kinetics

    OpenAIRE

    Feliu, Elisenda; Wiuf, Carsten

    2011-01-01

    We consider chemical reaction networks taken with mass action kinetics. The steady states of such a system are solutions to a system of polynomial equations. Even for small systems the task of finding the solutions is daunting. We develop an algebraic framework and procedure for linear elimination of variables. The procedure reduces the variables in the system to a set of "core" variables by eliminating variables corresponding to a set of non-interacting species. The steady states are paramet...

  18. Miniaturized Mass-Spectrometry-Based Analysis System for Fully Automated Examination of Conditioned Cell Culture Media

    NARCIS (Netherlands)

    Weber, E.; Pinkse, M.W.H.; Bener-Aksam, E.; Vellekoop, M.J.; Verhaert, P.D.E.M.

    2012-01-01

    We present a fully automated setup for performing in-line mass spectrometry (MS) analysis of conditioned media in cell cultures, in particular focusing on the peptides therein. The goal is to assess peptides secreted by cells in different culture conditions. The developed system is compatible with M

  19. The balance between automation and human actions: The Sizewell B perspective

    International Nuclear Information System (INIS)

    Sizewell B is the first of a family of four similar 1100 MWe Pressurised Water Reactor Power Stations planned by the CEGB for sites around England. The civil and mechanical design of these power stations follows closely that of the Westinghouse/Bechtel designed Standardised Nuclear Unit Power Plant Stations (SNUPPS) units at Wolf Creek and Callaway in the United States, with changes where necessary to meet CEGB requirements or to comply with British licensing requirements. The electrical and C and I systems are, however, very different in detail from those of a typical US PWR power station. The functional specifications of the electrical and C and I systems are based upon those of the SNUPPS units but the designs of the systems have evolved under a number of influences including previous CEGB practice, unique British licensing requirements and the opportunities offered by modern systems using microprocessor technology. This paper presents a personal view of the possibilities and limitations of automation and human actions based on experience of the specification and design process. It also discusses the approach to automation which has been adopted for Sizewell B and the small family of PWR stations that is to follow it, briefly touches on the PWR teams current philosophy for accident management and the possible future development of advanced C and I systems for accident management and operator information. (author)

  20. Grand symmetry, spectral action and the Higgs mass

    Energy Technology Data Exchange (ETDEWEB)

    Devastato, Agostino [Dipartimento di Fisica, Università di Napoli Federico II,Monte S. Angelo, Via Cintia, 80126 Napoli (Italy); INFN, Sezione di Napoli,Monte S. Angelo, Via Cintia, 80126 Napoli (Italy); Lizzi, Fedele [Dipartimento di Fisica, Università di Napoli Federico II,Monte S. Angelo, Via Cintia, 80126 Napoli (Italy); INFN, Sezione di Napoli,Monte S. Angelo, Via Cintia, 80126 Napoli (Italy); Departament de Estructura i Constituents de la Matèria, Institut de Ciéncies del Cosmos, Universitat de Barcelona, Barcelona, Catalonia (Spain); Martinetti, Pierre [Dipartimento di Fisica, Università di Napoli Federico II,Monte S. Angelo, Via Cintia, 80126 Napoli (Italy); INFN, Sezione di Napoli,Monte S. Angelo, Via Cintia, 80126 Napoli (Italy)

    2014-01-10

    In the context of the spectral action and the noncommutative geometry approach to the standard model, we build a model based on a larger symmetry. With this grand symmetry it is natural to have the scalar field necessary to obtain the Higgs mass in the vicinity of 126 GeV. This larger symmetry mixes gauge and spin degrees of freedom without introducing extra fermions. Requiring the noncommutative space to be an almost commutative geometry (i.e. the product of manifold by a finite dimensional internal space) gives conditions for the breaking of this grand symmetry to the standard model.

  1. Grand symmetry, spectral action and the Higgs mass

    International Nuclear Information System (INIS)

    In the context of the spectral action and the noncommutative geometry approach to the standard model, we build a model based on a larger symmetry. With this grand symmetry it is natural to have the scalar field necessary to obtain the Higgs mass in the vicinity of 126 GeV. This larger symmetry mixes gauge and spin degrees of freedom without introducing extra fermions. Requiring the noncommutative space to be an almost commutative geometry (i.e. the product of manifold by a finite dimensional internal space) gives conditions for the breaking of this grand symmetry to the standard model

  2. Unified mass-action theory for virus neutralization and radioimmunology

    International Nuclear Information System (INIS)

    All ideas implicit in the papers since 1953 involved in applying mass-action thermodynamics to antibody- antigen reactions are unified by the use of: (a) the intermediary concept of extent of reaction; (b) the concept of intrinsic association constant; (c) a statistical analysis for probable complexes; and (d) identification of the complex or complexes that contribute to the bioassay. Several general theoretical examples are given that show the limitations of linear interpretations of equilibrium data. Two practical examples from the literature illustrate foot-and-mouth disease virus and influenza virus neutralization. (Auth.)

  3. Mass deformed world-sheet action of semi local vortices

    International Nuclear Information System (INIS)

    The mass deformed effective world-sheet theory of semi local vortices was constructed via the field theoretical method. By Euler-Lagrangian equations, the Ansatze for both the gauge field and the adjoint scalar were solved, this ensures that zero modes of vortices are minimal excitations of the system. Up to the 1/g2 order, all profiles are solved. The mass deformed effective action was obtained by integrating out the transverse plane of the vortex string. The effective theory interpolates between the local vortex and the lump. Respecting certain normalization conditions, the effective theory shows a Seiberg-like duality, which agrees with the result of the Kähler quotient construction

  4. Law of mass action for co-precipitation

    International Nuclear Information System (INIS)

    Coprecipitation is often understood as the incorporation of elements at trace concentrations into -initially pure- solid compounds. Coprecipitation has typically been used to identify radioactive isotopes. Coprecipitation can result in lowering solubility as compared to the solubility, when controlled by pure compounds. For this reason it is also important for geochemistry, waste management and de-pollution studies. The solid obtained with coprecipitation is a new homogeneous solid phase called solid solution. The 2 formula needed to calculate the aqueous solubility when controlled by the ideal ABb(1-x)Ccx solid solutions are Ks,B1-x*Ks,Cx equals [AzA]*[BzB]b(1-x)*[CzC]cx/((1-x)b(1-x)xcx) and Ks,C/Ks,B equals (1-x)b*[CzC]c/[BzB]b*xc), where Ks,B and Ks,C are the classical constant solubility products of the ABb and ACc end-members, the b and c values are calculated from the (zi) charges of the ions and from charge balance. This report is essentially written to provide a thermodynamic demonstration of the law of mass action in attempts to confirm scientific bases for solubility calculations in geosciences (as typically retention of radio-nuclides by co-precipitation), and to facilitate such calculations. Note that the law of mass action is here a set of 2 equations (not only 1) for the ideal or near ideal systems. Since they are consistent with the phase rule, no extra formula (beside mass balance) is needed to calculate the concentrations of all the species in both phases, namely: [AzA], [BzB], [CzC] and specially x

  5. Mass spectra-based framework for automated structural elucidation of metabolome data to explore phytochemical diversity

    Directory of Open Access Journals (Sweden)

    Fumio eMatsuda

    2011-08-01

    Full Text Available A novel framework for automated elucidation of metabolite structures in liquid chromatography-mass spectrometer (LC-MS metabolome data was constructed by integrating databases. High-resolution tandem mass spectra data automatically acquired from each metabolite signal were used for database searches. Three distinct databases, KNApSAcK, ReSpect, and the PRIMe standard compound database, were employed for the structural elucidation. The outputs were retrieved using the CAS metabolite identifier for identification and putative annotation. A simple metabolite ontology system was also introduced to attain putative characterization of the metabolite signals. The automated method was applied for the metabolome data sets obtained from the rosette leaves of 20 Arabidopsis accessions. Phenotypic variations in novel Arabidopsis metabolites among these accessions could be investigated using this method.

  6. Sample Tracking in an Automated Cytogenetic Biodosimetry Laboratory for Radiation Mass Casualties

    OpenAIRE

    Martin, P.R.; Berdychevski, R.E.; Subramanian, U.; Blakely, W F; Prasanna, P.G.S.

    2007-01-01

    Chromosome aberration-based dicentric assay is expected to be used after mass casualty life-threatening radiation exposures to assess radiation dose to individuals. This will require processing of a large number of samples for individual dose assessment and clinical triage to aid treatment decisions. We have established an automated, high-throughput, cytogenetic biodosimetry laboratory to process a large number of samples for conducting the dicentric assay using peripheral blood from exposed ...

  7. Variable elimination in chemical reaction networks with mass action kinetics

    CERN Document Server

    Feliu, Elisenda

    2011-01-01

    We consider chemical reaction networks taken with mass action kinetics. The steady states of such a system are solutions to a system of polynomial equations. Even for small systems the task of finding the solutions is daunting. We develop an algebraic framework and procedure for linear elimination of variables. The procedure reduces the variables in the system to a set of "core" variables by eliminating variables corresponding to a set of non-interacting species. The steady states are parameterized algebraically by the core variables, and a graphical condition is given for when a steady state with positive core variables necessarily have all variables positive. Further, we characterize graphically the sets of eliminated variables that are constrained by a conservation law and show that this conservation law takes a specific form.

  8. Automated method for egg collection and new pupae separation device for medfly mass production

    International Nuclear Information System (INIS)

    The Sterile Insect Technique (SIT) is an environmentally friendly approach for suppression or eradication of insect pests and is used in many area-wide integrated programmes against tephritid fruit fly pests, particularly the Mediterranean fruit fly, Ceratitis capitata (Wiedemann). The mass-rearing procedures, used in specialized sterile medfly males producing facilities, still require considerable physical labor. Increasing demand for sterile males of high quality and acceptable cost for extending SIT programmes require a reduction of human labor by automation of the production process or some of its parts. The aim of this work was to develop an automated system for egg collection, as well as a new device for the separation of pupae from the pupation medium (sawdust). Both devices were tested in the medfly lab at the Institute of Zoology, SAS. Automated system for egg collection: The automated egg collecting system includes continuous collection of produced eggs in gentle stream of water, which permanently circulates in collecting channels. The eggs accumulate in collecting pots, which have a bottom constructed from soft mesh. Eggs are gently washed by a soft stream of well-oxygenated water and can be easily collected for the subsequent bubbling procedure. The automated egg collecting system consists of the following components: collecting channels, holders of collecting channels, water inlet, water pump, egg collecting pot and water reservoir. The developed new egg collecting system is fully automated and egg collecting is the only needed manual labor. Operating of the new egg collecting system is simpler and can be done in a shorter time than in other systems. Currently used separators usually utilise vibration to separate the pupae from the pupation medium. It results in a dusty environment in the pupae separation area. In the developed separator, the sawdust is exhausted in the initial phase of the sawdust-pupae mixture movement on the endless belt and small

  9. Law of mass action for co-precipitation; Loi d'action de masse de la co-precipitation

    Energy Technology Data Exchange (ETDEWEB)

    Vitorge, P

    2008-07-01

    Coprecipitation is often understood as the incorporation of elements at trace concentrations into -initially pure- solid compounds. Coprecipitation has typically been used to identify radioactive isotopes. Coprecipitation can result in lowering solubility as compared to the solubility, when controlled by pure compounds. For this reason it is also important for geochemistry, waste management and de-pollution studies. The solid obtained with coprecipitation is a new homogeneous solid phase called solid solution. The 2 formula needed to calculate the aqueous solubility when controlled by the ideal AB{sub b(1-x)}C{sub cx} solid solutions are K{sub s,B}{sup 1-x}*K{sub s,C}{sup x} equals [A{sup z{sub A}}]*[B{sup z{sub B}}]{sup b(1-x)}*[C{sup z{sub C}}]{sup cx}/((1-x){sup b(1-x)}x{sup cx}) and K{sub s,C}/K{sub s,B} equals (1-x){sup b}*[C{sup z{sub C}}]{sup c}/[B{sup z{sub B}}]{sup b}*x{sup c}), where K{sub s,B} and K{sub s,C} are the classical constant solubility products of the AB{sub b} and AC{sub c} end-members, the b and c values are calculated from the (z{sub i}) charges of the ions and from charge balance. This report is essentially written to provide a thermodynamic demonstration of the law of mass action in attempts to confirm scientific bases for solubility calculations in geosciences (as typically retention of radio-nuclides by co-precipitation), and to facilitate such calculations. Note that the law of mass action is here a set of 2 equations (not only 1) for the ideal or near ideal systems. Since they are consistent with the phase rule, no extra formula (beside mass balance) is needed to calculate the concentrations of all the species in both phases, namely: [A{sup z{sub A}}], [B{sup z{sub B}}], [C{sup z{sub C}}] and specially x.

  10. Automated probe microdistillation/mass spectrometry for the analysis of high-molecular weight compounds in fossil fuels

    Energy Technology Data Exchange (ETDEWEB)

    Scheppele, S.E.; Grindstaff, Q.G.; Grigsby, R.D.; Chung, K.C.; Hwang, C.S.; Marriott, T.D.; Schronk, L.R.

    1982-08-01

    Automated probe microdistillation/mass spectroscopy is described. The application of computer hardware and the development of computer software eliminate the dilatory nature of acquiring both the temperature and associated mass spectra and reduces the acquired data for processing. The interaction of various hardware components of the system as well as the associated software is described. (DMC)

  11. Automated work-flow for processing high-resolution direct infusion electrospray ionization mass spectral fingerprints

    DEFF Research Database (Denmark)

    Hansen, Michael Adsetts Edberg; Smedsgaard, Jørn

    2007-01-01

    automated data processing pipeline to compare large numbers of fingerprint spectra from direct infusion experiments analyzed by high resolution MS. We describe some of the intriguing problems that have to be addressed. starting with the conversion and pre-processing of the raw data to the final data...... analysis. Illustrated on the direct infusion analysis (ESI-TOF-MS) of complex mixtures the method exploits the full quality of the high-resolution present in the mass spectra. Although the method is illustrated as a new library search method for high resolution MS, we demonstrate that the output of the...... preprocessing is applicable to cluster-, discriminant analysis, and related multivariate methods applied directly to mass spectra from direct infusion analysis of crude extracts. This is done to find the relationship between several terverticillate Penicillium species and identify the ions responsible for the...

  12. Mass asymmetry and tricyclic wobble motion assessment using automated launch video analysis

    Institute of Scientific and Technical Information of China (English)

    Ryan DECKER; Joseph DONINI; William GARDNER; Jobin JOHN; Walter KOENIG

    2016-01-01

    This paper describes an approach to identify epicyclic and tricyclic motion during projectile flight caused by mass asymmetries in spin-stabilized projectiles. Flight video was captured following projectile launch of several M110A2E1 155 mm artillery projectiles. These videos were then analyzed using the automated flight video analysis method to attain their initial position and orientation histories. Examination of the pitch and yaw histories clearly indicates that in addition to epicyclic motion’s nutation and precession oscillations, an even faster wobble amplitude is present during each spin revolution, even though some of the amplitudes of the oscillation are smaller than 0.02 degree. The results are compared to a sequence of shots where little appreciable mass asymmetries were present, and only nutation and precession frequencies are predominantly apparent in the motion history results. Magnitudes of the wobble motion are estimated and compared to product of inertia measurements of the asymmetric projectiles.

  13. An Automated Platform for High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Lanekoff, Ingela T.; Heath, Brandi S.; Liyu, Andrey V.; Thomas, Mathew; Carson, James P.; Laskin, Julia

    2012-10-02

    An automated platform has been developed for acquisition and visualization of mass spectrometry imaging (MSI) data using nanospray desorption electrospray ionization (nano-DESI). The new system enables robust operation of the nano-DESI imaging source over many hours. This is achieved by controlling the distance between the sample and the probe by mounting the sample holder onto an automated XYZ stage and defining the tilt of the sample plane. This approach is useful for imaging of relatively flat samples such as thin tissue sections. Custom software called MSI QuickView was developed for visualization of large data sets generated in imaging experiments. MSI QuickView enables fast visualization of the imaging data during data acquisition and detailed processing after the entire image is acquired. The performance of the system is demonstrated by imaging rat brain tissue sections. High resolution mass analysis combined with MS/MS experiments enabled identification of lipids and metabolites in the tissue section. In addition, high dynamic range and sensitivity of the technique allowed us to generate ion images of low-abundance isobaric lipids. High-spatial resolution image acquired over a small region of the tissue section revealed the spatial distribution of an abundant brain metabolite, creatine, in the white and gray matter that is consistent with the literature data obtained using magnetic resonance spectroscopy.

  14. Application of automated mass spectrometry deconvolution and identification software for pesticide analysis in surface waters.

    Science.gov (United States)

    Furtula, Vesna; Derksen, George; Colodey, Alan

    2006-01-01

    A new approach to surface water analysis has been investigated in order to enhance the detection of different organic contaminants in Nathan Creek, British Columbia. Water samples from Nathan Creek were prepared by liquid/liquid extraction using dichloromethane (DCM) as an extraction solvent and analyzed by gas chromatography mass spectrometry method in scan mode (GC-MS scan). To increase sensitivity for pesticides detection, acquired scan data were further analyzed by Automated Mass Spectrometry Deconvolution and Identification Software (AMDIS) incorporated into the Agilent Deconvolution Reporting Software (DRS), which also includes mass spectral libraries for 567 pesticides. Extracts were reanalyzed by gas chromatography mass spectrometry single ion monitoring (GC-MS-SIM) to confirm and quantitate detected pesticides. Pesticides: atrazine, dimethoate, diazinone, metalaxyl, myclobutanil, napropamide, oxadiazon, propazine and simazine were detected at three sampling sites on the mainstream of the Nathan Creek. Results of the study are further discussed in terms of detectivity and identification level for each pesticide found. The proposed approach of monitoring pesticides in surface waters enables their detection and identification at trace levels. PMID:17090491

  15. FluTyper-an algorithm for automated typing and subtyping of the influenza virus from high resolution mass spectral data

    OpenAIRE

    Schwahn Alexander B; Wong Jason WH; Downard Kevin M

    2010-01-01

    Abstract Background High resolution mass spectrometry has been employed to rapidly and accurately type and subtype influenza viruses. The detection of signature peptides with unique theoretical masses enables the unequivocal assignment of the type and subtype of a given strain. This analysis has, to date, required the manual inspection of mass spectra of whole virus and antigen digests. Results A computer algorithm, FluTyper, has been designed and implemented to achieve the automated analysis...

  16. Automated thermochemolysis reactor for detection of Bacillus anthracis endospores by gas chromatography–mass spectrometry

    International Nuclear Information System (INIS)

    Graphical abstract: -- Highlights: •An automated sample preparation system for Bacillus anthracis endospores was developed. •A thermochemolysis method was applied to produce and derivatize biomarkers for Bacillus anthracis detection. •The autoreactor controlled the precise delivery of reagents, and TCM reaction times and temperatures. •Solid phase microextraction was used to extract biomarkers, and GC–MS was used for final identification. •This autoreactor was successfully applied to the identification of Bacillus anthracis endospores. -- Abstract: An automated sample preparation system was developed and tested for the rapid detection of Bacillus anthracis endospores by gas chromatography–mass spectrometry (GC–MS) for eventual use in the field. This reactor is capable of automatically processing suspected bio-threat agents to release and derivatize unique chemical biomarkers by thermochemolysis (TCM). The system automatically controls the movement of sample vials from one position to another, crimping of septum caps onto the vials, precise delivery of reagents, and TCM reaction times and temperatures. The specific operations of introduction of sample vials, solid phase microextraction (SPME) sampling, injection into the GC–MS system, and ejection of used vials from the system were performed manually in this study, although they can be integrated into the automated system. Manual SPME sampling is performed by following visual and audible signal prompts for inserting the fiber into and retracting it from the sampling port. A rotating carousel design allows for simultaneous sample collection, reaction, biomarker extraction and analysis of sequential samples. Dipicolinic acid methyl ester (DPAME), 3-methyl-2-butenoic acid methyl ester (a fragment of anthrose) and two methylated sugars were used to compare the performance of the autoreactor with manual TCM. Statistical algorithms were used to construct reliable bacterial endospore signatures, and 24

  17. Synergy of automation and human action in fuel handling control system of 500 MWe PHWR

    International Nuclear Information System (INIS)

    On-line refueling process is carried out in PHWRs on a routine basis. Each refueling cycle involves a large number of sequential operations to be executed safely and satisfactorily and hence is time consuming. Operations on the system are performed as per the detailed prescribed procedures. This necessitates introduction of automation of these operations; however the extent of automation has to be based on consideration of professional motivation and psychological well-being of the operator. This paper describes the nature of fuel handling operations and how man and machine work together in a complementary manner to achieve the objectives. (author). 2 refs., 7 figs., 1 tab

  18. Towards Automation 2.0: A Neurocognitive Model for Environment Recognition, Decision-Making, and Action Execution

    Directory of Open Access Journals (Sweden)

    Zucker Gerhard

    2011-01-01

    Full Text Available The ongoing penetration of building automation by information technology is by far not saturated. Today's systems need not only be reliable and fault tolerant, they also have to regard energy efficiency and flexibility in the overall consumption. Meeting the quality and comfort goals in building automation while at the same time optimizing towards energy, carbon footprint and cost-efficiency requires systems that are able to handle large amounts of information and negotiate system behaviour that resolves conflicting demands—a decision-making process. In the last years, research has started to focus on bionic principles for designing new concepts in this area. The information processing principles of the human mind have turned out to be of particular interest as the mind is capable of processing huge amounts of sensory data and taking adequate decisions for (re-actions based on these analysed data. In this paper, we discuss how a bionic approach can solve the upcoming problems of energy optimal systems. A recently developed model for environment recognition and decision-making processes, which is based on research findings from different disciplines of brain research is introduced. This model is the foundation for applications in intelligent building automation that have to deal with information from home and office environments. All of these applications have in common that they consist of a combination of communicating nodes and have many, partly contradicting goals.

  19. Y-12 Plant remedial action Technology Logic Diagram: Volume 3, Technology evaluation data sheets: Part B, Characterization; robotics/automation

    International Nuclear Information System (INIS)

    The Y-12 Plant Remedial Action Technology Logic Diagram (TLD) was developed to provide a decision-support tool that relates environmental restoration (ER) problems at the Y-12 Plant to potential technologies that can remediate theses problems. The TLD identifies the research, development, demonstration, testing, and evaluation needed for sufficient development of these technologies to allow for technology transfer and application to remedial action (RA) activities. The TLD consists of three volumes. Volume 1 contains an overview of the TLD, an explanation of the program-specific responsibilities, a review of identified technologies, and the rankings of remedial technologies. Volume 2 contains the logic linkages among environmental management goals, environmental problems, and the various technologies that have the potential to solve these problems. Volume 3 contains the TLD data sheets. This report is Part B of Volume 3 and contains the Characterization and Robotics/Automation sections

  20. Y-12 Plant remedial action Technology Logic Diagram: Volume 3, Technology evaluation data sheets: Part B, Characterization; robotics/automation

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-09-01

    The Y-12 Plant Remedial Action Technology Logic Diagram (TLD) was developed to provide a decision-support tool that relates environmental restoration (ER) problems at the Y-12 Plant to potential technologies that can remediate theses problems. The TLD identifies the research, development, demonstration, testing, and evaluation needed for sufficient development of these technologies to allow for technology transfer and application to remedial action (RA) activities. The TLD consists of three volumes. Volume 1 contains an overview of the TLD, an explanation of the program-specific responsibilities, a review of identified technologies, and the rankings of remedial technologies. Volume 2 contains the logic linkages among environmental management goals, environmental problems, and the various technologies that have the potential to solve these problems. Volume 3 contains the TLD data sheets. This report is Part B of Volume 3 and contains the Characterization and Robotics/Automation sections.

  1. Pep2Path: automated mass spectrometry-guided genome mining of peptidic natural products.

    Directory of Open Access Journals (Sweden)

    Marnix H Medema

    2014-09-01

    Full Text Available Nonribosomally and ribosomally synthesized bioactive peptides constitute a source of molecules of great biomedical importance, including antibiotics such as penicillin, immunosuppressants such as cyclosporine, and cytostatics such as bleomycin. Recently, an innovative mass-spectrometry-based strategy, peptidogenomics, has been pioneered to effectively mine microbial strains for novel peptidic metabolites. Even though mass-spectrometric peptide detection can be performed quite fast, true high-throughput natural product discovery approaches have still been limited by the inability to rapidly match the identified tandem mass spectra to the gene clusters responsible for the biosynthesis of the corresponding compounds. With Pep2Path, we introduce a software package to fully automate the peptidogenomics approach through the rapid Bayesian probabilistic matching of mass spectra to their corresponding biosynthetic gene clusters. Detailed benchmarking of the method shows that the approach is powerful enough to correctly identify gene clusters even in data sets that consist of hundreds of genomes, which also makes it possible to match compounds from unsequenced organisms to closely related biosynthetic gene clusters in other genomes. Applying Pep2Path to a data set of compounds without known biosynthesis routes, we were able to identify candidate gene clusters for the biosynthesis of five important compounds. Notably, one of these clusters was detected in a genome from a different subphylum of Proteobacteria than that in which the molecule had first been identified. All in all, our approach paves the way towards high-throughput discovery of novel peptidic natural products. Pep2Path is freely available from http://pep2path.sourceforge.net/, implemented in Python, licensed under the GNU General Public License v3 and supported on MS Windows, Linux and Mac OS X.

  2. A completely automated CAD system for mass detection in a large mammographic database

    International Nuclear Information System (INIS)

    Mass localization plays a crucial role in computer-aided detection (CAD) systems for the classification of suspicious regions in mammograms. In this article we present a completely automated classification system for the detection of masses in digitized mammographic images. The tool system we discuss consists in three processing levels: (a) Image segmentation for the localization of regions of interest (ROIs). This step relies on an iterative dynamical threshold algorithm able to select iso-intensity closed contours around gray level maxima of the mammogram. (b) ROI characterization by means of textural features computed from the gray tone spatial dependence matrix (GTSDM), containing second-order spatial statistics information on the pixel gray level intensity. As the images under study were recorded in different centers and with different machine settings, eight GTSDM features were selected so as to be invariant under monotonic transformation. In this way, the images do not need to be normalized, as the adopted features depend on the texture only, rather than on the gray tone levels, too. (c) ROI classification by means of a neural network, with supervision provided by the radiologist's diagnosis. The CAD system was evaluated on a large database of 3369 mammographic images [2307 negative, 1062 pathological (or positive), containing at least one confirmed mass, as diagnosed by an expert radiologist]. To assess the performance of the system, receiver operating characteristic (ROC) and free-response ROC analysis were employed. The area under the ROC curve was found to be Az=0.783±0.008 for the ROI-based classification. When evaluating the accuracy of the CAD against the radiologist-drawn boundaries, 4.23 false positives per image are found at 80% of mass sensitivity

  3. Pep2Path: automated mass spectrometry-guided genome mining of peptidic natural products.

    Science.gov (United States)

    Medema, Marnix H; Paalvast, Yared; Nguyen, Don D; Melnik, Alexey; Dorrestein, Pieter C; Takano, Eriko; Breitling, Rainer

    2014-09-01

    Nonribosomally and ribosomally synthesized bioactive peptides constitute a source of molecules of great biomedical importance, including antibiotics such as penicillin, immunosuppressants such as cyclosporine, and cytostatics such as bleomycin. Recently, an innovative mass-spectrometry-based strategy, peptidogenomics, has been pioneered to effectively mine microbial strains for novel peptidic metabolites. Even though mass-spectrometric peptide detection can be performed quite fast, true high-throughput natural product discovery approaches have still been limited by the inability to rapidly match the identified tandem mass spectra to the gene clusters responsible for the biosynthesis of the corresponding compounds. With Pep2Path, we introduce a software package to fully automate the peptidogenomics approach through the rapid Bayesian probabilistic matching of mass spectra to their corresponding biosynthetic gene clusters. Detailed benchmarking of the method shows that the approach is powerful enough to correctly identify gene clusters even in data sets that consist of hundreds of genomes, which also makes it possible to match compounds from unsequenced organisms to closely related biosynthetic gene clusters in other genomes. Applying Pep2Path to a data set of compounds without known biosynthesis routes, we were able to identify candidate gene clusters for the biosynthesis of five important compounds. Notably, one of these clusters was detected in a genome from a different subphylum of Proteobacteria than that in which the molecule had first been identified. All in all, our approach paves the way towards high-throughput discovery of novel peptidic natural products. Pep2Path is freely available from http://pep2path.sourceforge.net/, implemented in Python, licensed under the GNU General Public License v3 and supported on MS Windows, Linux and Mac OS X. PMID:25188327

  4. Automated Glycan Sequencing from Tandem Mass Spectra of N-Linked Glycopeptides.

    Science.gov (United States)

    Yu, Chuan-Yih; Mayampurath, Anoop; Zhu, Rui; Zacharias, Lauren; Song, Ehwang; Wang, Lei; Mechref, Yehia; Tang, Haixu

    2016-06-01

    Mass spectrometry has become a routine experimental tool for proteomic biomarker analysis of human blood samples, partly due to the large availability of informatics tools. As one of the most common protein post-translational modifications (PTMs) in mammals, protein glycosylation has been observed to alter in multiple human diseases and thus may potentially be candidate markers of disease progression. While mass spectrometry instrumentation has seen advancements in capabilities, discovering glycosylation-related markers using existing software is currently not straightforward. Complete characterization of protein glycosylation requires the identification of intact glycopeptides in samples, including identification of the modification site as well as the structure of the attached glycans. In this paper, we present GlycoSeq, an open-source software tool that implements a heuristic iterated glycan sequencing algorithm coupled with prior knowledge for automated elucidation of the glycan structure within a glycopeptide from its collision-induced dissociation tandem mass spectrum. GlycoSeq employs rules of glycosidic linkage as defined by glycan synthetic pathways to eliminate improbable glycan structures and build reasonable glycan trees. We tested the tool on two sets of tandem mass spectra of N-linked glycopeptides cell lines acquired from breast cancer patients. After employing enzymatic specificity within the N-linked glycan synthetic pathway, the sequencing results of GlycoSeq were highly consistent with the manually curated glycan structures. Hence, GlycoSeq is ready to be used for the characterization of glycan structures in glycopeptides from MS/MS analysis. GlycoSeq is released as open source software at https://github.com/chpaul/GlycoSeq/ . PMID:27111718

  5. An Efficient Approach for Automated Mass Segmentation and Classification in Mammograms.

    Science.gov (United States)

    Dong, Min; Lu, Xiangyu; Ma, Yide; Guo, Yanan; Ma, Yurun; Wang, Keju

    2015-10-01

    Breast cancer is becoming a leading death of women all over the world; clinical experiments demonstrate that early detection and accurate diagnosis can increase the potential of treatment. In order to improve the breast cancer diagnosis precision, this paper presents a novel automated segmentation and classification method for mammograms. We conduct the experiment on both DDSM database and MIAS database, firstly extract the region of interests (ROIs) with chain codes and using the rough set (RS) method to enhance the ROIs, secondly segment the mass region from the location ROIs with an improved vector field convolution (VFC) snake and following extract features from the mass region and its surroundings, and then establish features database with 32 dimensions; finally, these features are used as input to several classification techniques. In our work, the random forest is used and compared with support vector machine (SVM), genetic algorithm support vector machine (GA-SVM), particle swarm optimization support vector machine (PSO-SVM), and decision tree. The effectiveness of our method is evaluated by a comprehensive and objective evaluation system; also, Matthew's correlation coefficient (MCC) indicator is used. Among the state-of-the-art classifiers, our method achieves the best performance with best accuracy of 97.73%, and the MCC value reaches 0.8668 and 0.8652 in unique DDSM database and both two databases, respectively. Experimental results prove that the proposed method outperforms the other methods; it could consider applying in CAD systems to assist the physicians for breast cancer diagnosis. PMID:25776767

  6. A rapid, automated approach to optimisation of multiple reaction monitoring conditions for quantitative bioanalytical mass spectrometry.

    Science.gov (United States)

    Higton, D M

    2001-01-01

    An improvement to the procedure for the rapid optimisation of mass spectrometry (PROMS), for the development of multiple reaction methods (MRM) for quantitative bioanalytical liquid chromatography/tandem mass spectrometry (LC/MS/MS), is presented. PROMS is an automated protocol that uses flow-injection analysis (FIA) and AppleScripts to create methods and acquire the data for optimisation. The protocol determines the optimum orifice potential, the MRM conditions for each compound, and finally creates the MRM methods needed for sample analysis. The sensitivities of the MRM methods created by PROMS approach those created manually. MRM method development using PROMS currently takes less than three minutes per compound compared to at least fifteen minutes manually. To further enhance throughput, approaches to MRM optimisation using one injection per compound, two injections per pool of five compounds and one injection per pool of five compounds have been investigated. No significant difference in the optimised instrumental parameters for MRM methods were found between the original PROMS approach and these new methods, which are up to ten times faster. The time taken for an AppleScript to determine the optimum conditions and build the MRM methods is the same with all approaches. PMID:11596136

  7. Automated combustion accelerator mass spectrometry for the analysis of biomedical samples in the low attomole range.

    Science.gov (United States)

    van Duijn, Esther; Sandman, Hugo; Grossouw, Dimitri; Mocking, Johannes A J; Coulier, Leon; Vaes, Wouter H J

    2014-08-01

    The increasing role of accelerator mass spectrometry (AMS) in biomedical research necessitates modernization of the traditional sample handling process. AMS was originally developed and used for carbon dating, therefore focusing on a very high precision but with a comparably low sample throughput. Here, we describe the combination of automated sample combustion with an elemental analyzer (EA) online coupled to an AMS via a dedicated interface. This setup allows direct radiocarbon measurements for over 70 samples daily by AMS. No sample processing is required apart from the pipetting of the sample into a tin foil cup, which is placed in the carousel of the EA. In our system, up to 200 AMS analyses are performed automatically without the need for manual interventions. We present results on the direct total (14)C count measurements in <2 μL human plasma samples. The method shows linearity over a range of 0.65-821 mBq/mL, with a lower limit of quantification of 0.65 mBq/mL (corresponding to 0.67 amol for acetaminophen). At these extremely low levels of activity, it becomes important to quantify plasma specific carbon percentages. This carbon percentage is automatically generated upon combustion of a sample on the EA. Apparent advantages of the present approach include complete omission of sample preparation (reduced hands-on time) and fully automated sample analysis. These improvements clearly stimulate the standard incorporation of microtracer research in the drug development process. In combination with the particularly low sample volumes required and extreme sensitivity, AMS strongly improves its position as a bioanalysis method. PMID:25033319

  8. Possibilities of the common research-development action in the field of automated logistical engines

    Directory of Open Access Journals (Sweden)

    Pap Lajos

    2003-12-01

    Full Text Available The paper briefly presents the R&D cooperation of the Department of Materials Handling and Logistics and Departments of Automation. The main fields of cooperation are introduced. Different kind of Linear Motor (hereafter LM drives are being developed and tested for warehouse and rolling conveyor systems. Modern control strategies using AI methods are being investigated and tested for Automated guide vehicle. Wireless communication methods are being searched and developed for mobile material handling devices. Application possibilities of voice recognition and image processing are being tested for control of material handling robots and devices. Application of process visualization programs are being developed and investigated. Multi-level industrial communication system is being developed for the laboratories of the cooperating departments.

  9. A Projection Argument for Differential Inclusions, with Applications to Persistence of Mass-Action Kinetics

    Directory of Open Access Journals (Sweden)

    Manoj Gopalkrishnan

    2013-03-01

    Full Text Available Motivated by questions in mass-action kinetics, we introduce the notion of vertexical family of differential inclusions. Defined on open hypercubes, these families are characterized by particular good behavior under projection maps. The motivating examples are certain families of reaction networks – including reversible, weakly reversible, endotactic, and strongly endotactic reaction networks – that give rise to vertexical families of mass-action differential inclusions. We prove that vertexical families are amenable to structural induction. Consequently, a trajectory of a vertexical family approaches the boundary if and only if either the trajectory approaches a vertex of the hypercube, or a trajectory in a lower-dimensional member of the family approaches the boundary. With this technology, we make progress on the global attractor conjecture, a central open problem concerning mass-action kinetics systems. Additionally, we phrase mass-action kinetics as a functor on reaction networks with variable rates.

  10. NPR determination of quark masses from the HISQ action

    CERN Document Server

    Lytle, Andrew T

    2015-01-01

    I report on a calculation of bilinear Z-factors needed for determining Z_m using non-perturbative renormalization (NPR) on n_f=2+1+1 HISQ ensembles. RI/MOM and RI/SMOM schemes are studied. These will provide an independent determination of quark masses in addition to other methods being used by the HPQCD collaboration.

  11. Mass Action Models Describing Extant Horizontal Transfer of Plasmids: Inferences and Parameter Sensitivities

    DEFF Research Database (Denmark)

    Smets, Barth F.; Lardon, Laurent

    Predicting the fate of horizontally transmissible elements in extant microbial communities might be facilitated by the availability of suitable mathematical models. Since the mid-1970s, mass action models have been introduced to describe the transfer of conjugal and mobilizable genetic elements...... these mathematical models. Finally, further permutations, as well as limitations of these mass action models in view of the structured complexity of most microbial systems are addressed....

  12. FluTyper-an algorithm for automated typing and subtyping of the influenza virus from high resolution mass spectral data

    Directory of Open Access Journals (Sweden)

    Schwahn Alexander B

    2010-05-01

    Full Text Available Abstract Background High resolution mass spectrometry has been employed to rapidly and accurately type and subtype influenza viruses. The detection of signature peptides with unique theoretical masses enables the unequivocal assignment of the type and subtype of a given strain. This analysis has, to date, required the manual inspection of mass spectra of whole virus and antigen digests. Results A computer algorithm, FluTyper, has been designed and implemented to achieve the automated analysis of MALDI mass spectra recorded for proteolytic digests of the whole influenza virus and antigens. FluTyper incorporates the use of established signature peptides and newly developed naïve Bayes classifiers for four common influenza antigens, hemagglutinin, neuraminidase, nucleoprotein, and matrix protein 1, to type and subtype the influenza virus based on their detection within proteolytic peptide mass maps. Theoretical and experimental testing of the classifiers demonstrates their applicability at protein coverage rates normally achievable in mass mapping experiments. The application of FluTyper to whole virus and antigen digests of a range of different strains of the influenza virus is demonstrated. Conclusions FluTyper algorithm facilitates the rapid and automated typing and subtyping of the influenza virus from mass spectral data. The newly developed naïve Bayes classifiers increase the confidence of influenza virus subtyping, especially where signature peptides are not detected. FluTyper is expected to popularize the use of mass spectrometry to characterize influenza viruses.

  13. A New Modular Strategy For Action Sequence Automation Using Neural Networks And Hidden Markov Models

    OpenAIRE

    Mohamed Adel Taher; Mostapha Abdeljawad

    2013-01-01

    In this paper, the authors propose a new hybrid strategy (using artificial neural networks and hidden Markov models) for skill automation. The strategy is based on the concept of using an “adaptive desired†that is introduced in the paper. The authors explain how using an adaptive desired can help a system for which an explicit model is not available or is difficult to obtain to smartly cope with environmental disturbances without requiring explicit rules specification (as with fuzzy syste...

  14. A new method for automated discontinuity trace mapping on rock mass 3D surface model

    Science.gov (United States)

    Li, Xiaojun; Chen, Jianqin; Zhu, Hehua

    2016-04-01

    This paper presents an automated discontinuity trace mapping method on a 3D surface model of rock mass. Feature points of discontinuity traces are first detected using the Normal Tensor Voting Theory, which is robust to noisy point cloud data. Discontinuity traces are then extracted from feature points in four steps: (1) trace feature point grouping, (2) trace segment growth, (3) trace segment connection, and (4) redundant trace segment removal. A sensitivity analysis is conducted to identify optimal values for the parameters used in the proposed method. The optimal triangular mesh element size is between 5 cm and 6 cm; the angle threshold in the trace segment growth step is between 70° and 90°; the angle threshold in the trace segment connection step is between 50° and 70°, and the distance threshold should be at least 15 times the mean triangular mesh element size. The method is applied to the excavation face trace mapping of a drill-and-blast tunnel. The results show that the proposed discontinuity trace mapping method is fast and effective and could be used as a supplement to traditional direct measurement of discontinuity traces.

  15. Toward automated chromatographic fingerprinting: A non-alignment approach to gas chromatography mass spectrometry data.

    Science.gov (United States)

    Vestner, Jochen; de Revel, Gilles; Krieger-Weber, Sibylle; Rauhut, Doris; du Toit, Maret; de Villiers, André

    2016-03-10

    In contrast to targeted analysis of volatile compounds, non-targeted approaches take information of known and unknown compounds into account, are inherently more comprehensive and give a more holistic representation of the sample composition. Although several non-targeted approaches have been developed, there's still a demand for automated data processing tools, especially for complex multi-way data such as chromatographic data obtained from multichannel detectors. This work was therefore aimed at developing a data processing procedure for gas chromatography mass spectrometry (GC-MS) data obtained from non-targeted analysis of volatile compounds. The developed approach uses basic matrix manipulation of segmented GC-MS chromatograms and PARAFAC multi-way modelling. The approach takes retention time shifts and peak shape deformations between samples into account and can be done with the freely available N-way toolbox for MATLAB. A demonstration of the new fingerprinting approach is presented using an artificial GC-MS data set and an experimental full-scan GC-MS data set obtained for a set of experimental wines. PMID:26893085

  16. Automated MALDI Matrix Coating System for Multiple Tissue Samples for Imaging Mass Spectrometry

    Science.gov (United States)

    Mounfield, William P.; Garrett, Timothy J.

    2012-03-01

    Uniform matrix deposition on tissue samples for matrix-assisted laser desorption/ionization (MALDI) is key for reproducible analyte ion signals. Current methods often result in nonhomogenous matrix deposition, and take time and effort to produce acceptable ion signals. Here we describe a fully-automated method for matrix deposition using an enclosed spray chamber and spray nozzle for matrix solution delivery. A commercial air-atomizing spray nozzle was modified and combined with solenoid controlled valves and a Programmable Logic Controller (PLC) to control and deliver the matrix solution. A spray chamber was employed to contain the nozzle, sample, and atomized matrix solution stream, and to prevent any interference from outside conditions as well as allow complete control of the sample environment. A gravity cup was filled with MALDI matrix solutions, including DHB in chloroform/methanol (50:50) at concentrations up to 60 mg/mL. Various samples (including rat brain tissue sections) were prepared using two deposition methods (spray chamber, inkjet). A linear ion trap equipped with an intermediate-pressure MALDI source was used for analyses. Optical microscopic examination showed a uniform coating of matrix crystals across the sample. Overall, the mass spectral images gathered from tissues coated using the spray chamber system were of better quality and more reproducible than from tissue specimens prepared by the inkjet deposition method.

  17. Greazy: Open-Source Software for Automated Phospholipid Tandem Mass Spectrometry Identification.

    Science.gov (United States)

    Kochen, Michael A; Chambers, Matthew C; Holman, Jay D; Nesvizhskii, Alexey I; Weintraub, Susan T; Belisle, John T; Islam, M Nurul; Griss, Johannes; Tabb, David L

    2016-06-01

    Lipid identification from data produced with high-throughput technologies is essential to the elucidation of the roles played by lipids in cellular function and disease. Software tools for identifying lipids from tandem mass (MS/MS) spectra have been developed, but they are often costly or lack the sophistication of their proteomics counterparts. We have developed Greazy, an open source tool for the automated identification of phospholipids from MS/MS spectra, that utilizes methods similar to those developed for proteomics. From user-supplied parameters, Greazy builds a phospholipid search space and associated theoretical MS/MS spectra. Experimental spectra are scored against search space lipids with similar precursor masses using a peak score based on the hypergeometric distribution and an intensity score utilizing the percentage of total ion intensity residing in matching peaks. The LipidLama component filters the results via mixture modeling and density estimation. We assess Greazy's performance against the NIST 2014 metabolomics library, observing high accuracy in a search of multiple lipid classes. We compare Greazy/LipidLama against the commercial lipid identification software LipidSearch and show that the two platforms differ considerably in the sets of identified spectra while showing good agreement on those spectra identified by both. Lastly, we demonstrate the utility of Greazy/LipidLama with different instruments. We searched data from replicates of alveolar type 2 epithelial cells obtained with an Orbitrap and from human serum replicates generated on a quadrupole-time-of-flight (Q-TOF). These findings substantiate the application of proteomics derived methods to the identification of lipids. The software is available from the ProteoWizard repository: http://tiny.cc/bumbershoot-vc12-bin64 . PMID:27186799

  18. Strong coupling expansion for the mass gap in SU(2) lattice gauge theory with mixed action

    International Nuclear Information System (INIS)

    We perform a strong coupling expansion up to O(β7) for the mass gap in SU(2) lattice gauge theory with mixed action. A novel feature of the strong coupling expansion is discussed. The strong coupling series appears to approach the scaling region more smoothly and Pade approximants become more stable than in the case with simple Wilson action. The region of validity of a recently proposed resummation of perturbation theory as applied to the determination of the asymptotic scaling behavior is investigated. Results of a strong coupling calculation for the Heat Kernel action, which is related to the mixed action for a special choice of parameters are also reported. (orig.)

  19. Technical note: CT-guided biopsy of lung masses using an automated guiding apparatus

    International Nuclear Information System (INIS)

    Automated guiding apparatuses for CT-guided biopsies are now available. We report our experience with an indigenous system to guide lung biopsies. This system gave results similar to those with the manual technique. Automated planning also appears to be technically easier, it requires fewer number of needle passes, consumes less time, and requires fewer number of check scans

  20. From Diagnosis to Action: An Automated Failure Advisor for Human Deep Space Missions

    Science.gov (United States)

    Colombano, Silvano; Spirkovska, Lilly; Baskaran, Vijayakumar; Morris, Paul; Mcdermott, William; Ossenfort, John; Bajwa, Anupa

    2015-01-01

    The major goal of current space system development at NASA is to enable human travel to deep space locations such as Mars and asteroids. At that distance, round trip communication with ground operators may take close to an hour, thus it becomes unfeasible to seek ground operator advice for problems that require immediate attention, either for crew safety or for activities that need to be performed at specific times for the attainment of scientific results. To achieve this goal, major reliance will need to be placed on automation systems capable of aiding the crew in detecting and diagnosing failures, assessing consequences of these failures, and providing guidance in repair activities that may be required. We report here on the most current step in the continuing development of such a system, and that is the addition of a Failure Response Advisor. In simple terms, we have a system in place the Advanced Caution and Warning System (ACAWS) to tell us what happened (failure diagnosis) and what happened because that happened (failure effects). The Failure Response Advisor will tell us what to do about it, how long until something must be done and why its important that something be done and will begin to approach the complex reasoning that is generally required for an optimal approach to automated system health management. This advice is based on the criticality and various timing elements, such as durations of activities and of component repairs, failure effects delay, and other factors. The failure advice is provided to operators (crew and mission controllers) together with the diagnostic and effects information. The operators also have the option to drill down for more information about the failure and the reasons for any suggested priorities.

  1. Automated processing of whole blood samples for the determination of immunosuppressants by liquid chromatography tandem-mass spectrometry

    OpenAIRE

    Vogeser, Michael; Spöhrer, Ute

    2006-01-01

    Background: Liquid chromatography tandem-mass spectrometry (LC-MS/MS) is an efficient technology for routine determination of immunosuppressants in whole blood; however, time-consuming manual sample preparation remains a significant limitation of this technique. Methods: Using a commercially available robotic pipetting system (Tecan Freedom EVO), we developed an automated sample-preparation protocol for quantification of tacrolimus in whole blood by LC-MS/MS. Barcode reading, sample resuspens...

  2. Automated knowledge acquisition - an aid to understanding the actions of a skilled operator

    International Nuclear Information System (INIS)

    The operator of an accelerator experiment often appears to be a highly skilled magician. The diagnostics used, such as oscilloscope traces, may be only indirectly related to the state of the accelerator. The operator adjusts the knobs so that the experiment runs well, but is not always able to describe in words exactly what is happening. The tool described in this paper, acc-plot-tool, was developed as a supplement to note-taking while attempting to develop an expert system that might function as an apprentice operator. During an experiment, a historical record is made of all operator actions and the resulting changes in the state of the equipment, including digitized records of the oscilloscope traces used to make decisions. These records are transformed into a set of graphical objects in the knowledge engineering environment (KEE). A flexible set of KEE functions has been developed, allowing both graphical and numerical manipulation of the data. The acc-plot-tool is not only useful in helping to understand the operator's actions, but also in discovering correlations between operator actions and system-parameter changes, in detecting equipment failures and in the mathematical modeling of the system. Examples will be given from ion-source operation. (orig.)

  3. Fully Automated Laser Ablation Liquid Capture Sample Analysis using NanoElectrospray Ionization Mass Spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Lorenz, Matthias [ORNL; Ovchinnikova, Olga S [ORNL; Van Berkel, Gary J [ORNL

    2014-01-01

    RATIONALE: Laser ablation provides for the possibility of sampling a large variety of surfaces with high spatial resolution. This type of sampling when employed in conjunction with liquid capture followed by nanoelectrospray ionization provides the opportunity for sensitive and prolonged interrogation of samples by mass spectrometry as well as the ability to analyze surfaces not amenable to direct liquid extraction. METHODS: A fully automated, reflection geometry, laser ablation liquid capture spot sampling system was achieved by incorporating appropriate laser fiber optics and a focusing lens into a commercially available, liquid extraction surface analysis (LESA ) ready Advion TriVersa NanoMate system. RESULTS: Under optimized conditions about 10% of laser ablated material could be captured in a droplet positioned vertically over the ablation region using the NanoMate robot controlled pipette. The sampling spot size area with this laser ablation liquid capture surface analysis (LA/LCSA) mode of operation (typically about 120 m x 160 m) was approximately 50 times smaller than that achievable by direct liquid extraction using LESA (ca. 1 mm diameter liquid extraction spot). The set-up was successfully applied for the analysis of ink on glass and paper as well as the endogenous components in Alstroemeria Yellow King flower petals. In a second mode of operation with a comparable sampling spot size, termed laser ablation/LESA , the laser system was used to drill through, penetrate, or otherwise expose material beneath a solvent resistant surface. Once drilled, LESA was effective in sampling soluble material exposed at that location on the surface. CONCLUSIONS: Incorporating the capability for different laser ablation liquid capture spot sampling modes of operation into a LESA ready Advion TriVersa NanoMate enhanced the spot sampling spatial resolution of this device and broadened the surface types amenable to analysis to include absorbent and solvent resistant

  4. Investigating the kinematics of coronal mass ejections with the automated CORIMP catalog

    Science.gov (United States)

    Byrne, Jason P.

    2015-07-01

    Studying coronal mass ejections (CMEs) in coronagraph data can be challenging due to their diffuse structure and transient nature, compounded by the variations in their dynamics, morphology and frequency of occurrence. The large amounts of data available from missions like the Solar and Heliospheric Observatory (SOHO) make manual cataloging of CMEs tedious and prone to human error, and so a robust method of detection and analysis is required and often preferred. A new coronal image processing catalog called CORIMP has been developed in an effort to achieve this, through the implementation of a dynamic background separation technique and multiscale edge detection. These algorithms together isolate and characterise CME structure in the field-of-view of the Large Angle Spectrometric Coronagraph (LASCO) onboard SOHO. CORIMP also applies a Savitzky-Golay filter, along with quadratic and linear fits, to the height-time measurements for better revealing the true CME speed and acceleration profiles across the plane-of-sky. Here we present a sample of new results from the CORIMP CME catalog, and directly compare them with the other automated catalogs of Computer Aided CME Tracking (CACTus) and Solar Eruptive Events Detection System (SEEDS), as well as the manual CME catalog at the Coordinated Data Analysis Workshop (CDAW) Data Center and a previously published study of the sample events. We further investigate a form of unsupervised machine learning by using a k-means clustering algorithm to distinguish detections of multiple CMEs that occur close together in space and time. While challenges still exist, this investigation and comparison of results demonstrate the reliability and robustness of the CORIMP catalog, proving its effectiveness at detecting and tracking CMEs throughout the LASCO dataset.

  5. Back action evading quantum measurement of motion in a negative mass reference frame

    CERN Document Server

    Møller, Christoffer B; Vasilakis, Georgios; Zeuthen, Emil; Tsaturyan, Yeghishe; Jensen, Kasper; Schliesser, Albert; Hammerer, Klemens; Polzik, Eugene S

    2016-01-01

    Quantum mechanics dictates that a measurement without perturbation is not possible. A textbook example is the observation of the position of an object, which imposes a random back action perturbation on the momentum. This randomness translates with time into position uncertainty, thus leading to the well known uncertainty on the measurement of motion. Here we demonstrate that the back action on an oscillator measured in a reference frame of another oscillator with an effective negative mass can be evaded in both position and momentum variables simultaneously. The mechanical oscillator is a millimeter-sized membrane and the reference negative mass oscillator is the collective spin of an atomic ensemble precessing in a magnetic field. Laser light transmitted through the hybrid system of these two disparate oscillators serves as the meter. We first observe the quantum measurement back action on each oscillator. We then demonstrate that back action at the single noise photon level is efficiently suppressed or enh...

  6. Overlap valence quarks on a twisted mass sea: A case study for mixed action lattice QCD

    Energy Technology Data Exchange (ETDEWEB)

    Cichy, Krzysztof, E-mail: krzysztof.cichy@desy.de [NIC, DESY, Platanenallee 6, D-15738 Zeuthen (Germany); Adam Mickiewicz University, Faculty of Physics, Umultowska 85, 61-614 Poznan (Poland); Drach, Vincent, E-mail: vincent.drach@desy.de [NIC, DESY, Platanenallee 6, D-15738 Zeuthen (Germany); García-Ramos, Elena, E-mail: elena.garcia.ramos@desy.de [NIC, DESY, Platanenallee 6, D-15738 Zeuthen (Germany); Humboldt Universität zu Berlin, Newtonstr. 15, 12489 Berlin (Germany); Herdoíza, Gregorio, E-mail: gregorio.herdoiza@uam.es [Departamento de Física Teórica and Instituto de Física Teórica, UAM/CSIC, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid (Spain); Jansen, Karl, E-mail: karl.jansen@desy.de [NIC, DESY, Platanenallee 6, D-15738 Zeuthen (Germany)

    2013-04-11

    We discuss a lattice QCD mixed action investigation employing Wilson maximally twisted mass sea and overlap valence fermions. Using four values of the lattice spacing, we demonstrate that the overlap Dirac operator assumes a point-like locality in the continuum limit. We also show that by adopting suitable matching conditions for the sea and valence theories a consistent continuum limit for the pion decay constant and light baryon masses can be obtained. Finally, we confront results for sea–valence mixed meson masses and the valence scalar correlator with corresponding expressions of chiral perturbation theory. This allows us to extract low energy constants of mixed action chiral perturbation which characterize the strength of unitarity violations in our mixed action setup.

  7. Overlap valence quarks on a twisted mass sea. A case study for mixed action lattice QCD

    Energy Technology Data Exchange (ETDEWEB)

    Cichy, Krzysztof [Deutsches Elektronen-Synchrotron (DESY), Zeuthen (Germany). John von Neumann-Inst. fuer Computing NIC; Poznan Univ. (Poland). Faculty of Physics; Drach, Vincent; Jansen, Karl [Deutsches Elektronen-Synchrotron (DESY), Zeuthen (Germany). John von Neumann-Inst. fuer Computing NIC; Garcia-Ramos, Elena [Deutsches Elektronen-Synchrotron (DESY), Zeuthen (Germany). John von Neumann-Inst. fuer Computing NIC; Humboldt-Universitaet, Berlin (Germany); Herdoiza, Gregorio [UAM/CSIC Univ. Autonoma de Madrid (Spain). Dept. de Fisica Teorica; UAM/CSIC Univ. Autonoma de Madrid (Spain). Inst. de Fisica Teorica; Collaboration: European Twisted Mass Collaboration

    2012-11-15

    We discuss a Lattice QCD mixed action investigation employing Wilson maximally twisted mass sea and overlap valence fermions. Using four values of the lattice spacing, we demonstrate that the overlap Dirac operator assumes a point-like locality in the continuum limit. We also show that by adopting suitable matching conditions for the sea and valence theories a consistent continuum limit for the pion decay constant and light baryon masses can be obtained. Finally, we confront results for sea-valence mixed meson masses and the valence scalar correlator with corresponding expressions of chiral perturbation theory. This allows us to extract low energy constants of mixed action chiral perturbation which characterize the strength of unitarity violations in our mixed action setup.

  8. Overlap valence quarks on a twisted mass sea. A case study for mixed action lattice QCD

    International Nuclear Information System (INIS)

    We discuss a Lattice QCD mixed action investigation employing Wilson maximally twisted mass sea and overlap valence fermions. Using four values of the lattice spacing, we demonstrate that the overlap Dirac operator assumes a point-like locality in the continuum limit. We also show that by adopting suitable matching conditions for the sea and valence theories a consistent continuum limit for the pion decay constant and light baryon masses can be obtained. Finally, we confront results for sea-valence mixed meson masses and the valence scalar correlator with corresponding expressions of chiral perturbation theory. This allows us to extract low energy constants of mixed action chiral perturbation which characterize the strength of unitarity violations in our mixed action setup.

  9. Numerical simulations with two flavours of twisted-mass Wilson quarks and DBW2 gauge action

    International Nuclear Information System (INIS)

    Discretisation errors in two-flavour lattice QCD with Wilson quarks and DBW2 gauge action are investigated by comparing numerical simulation data at two values of the bare gauge coupling. Both non-zero- and zero-twisted-mass values are considered. The results, including also data from simulations using the Wilson plaquette gauge action, are compared to next-to-leading order chiral perturbation theory formulas. (orig.)

  10. [Non-target screening of organic pollutants in sediments and sludges using gas chromatography-mass spectrometry and automated mass spectral deconvolution].

    Science.gov (United States)

    Wang, Gang; Ma, Huilian; Wang, Longxing; Chen, Jiping; Hou, Xiaohong

    2015-12-01

    A screening method in the combination of ultrasonic extraction, gas chromatography-mass spectrometry detection and automated mass spectrometry deconvolution technique was developed for non-target screening of non-polar and weak polar pollutants in sediments and sludges. The samples were extracted by ultrasonication for 20 min using dichloromethane for three times. The extraction solutions were cleaned-up by gel permeation chromatography and a silica gel column, and then 3 g of copper powder was used to remove the sulfur by ultrasonication for 10 min. Parallel experiments were carried out for 5 times and the RSDs were ranged from 5.8% to 14.9%. Automated mass spectral deconvolution & identification system (AMDIS) would improve the resolution of overlapping peaks, and identify the pure mass spectrum of the analytes in the cases of stronger background interference and co-extracted substances covering. Standard spectrum databases, such as NISTDRUG, NISTEPA, NISTFDA, Mass Spectral Library, etc, would qualitatively identify the organic pollutants in the samples. As a result, a total of 290 organic pollutants were identified, of which 190 and 153 pollutants were found in sediments and sludges, respectively. The identified pollutants included the Environmental Protection Agency (EPA) priority pollutants, pharmaceuticals, herbicides, antioxidants, intermediates, organic solvents and chemical raw materials. The proposed method is proved to be a promising one for non-target screening of complex matrix samples with the advantages of higher sensitivity and better repeatability. PMID:27097463

  11. Negative-parity baryon masses using an Ο(α)-improved fermion action

    International Nuclear Information System (INIS)

    We present a calculation of the mass of the lowest-lying negative-parity J = 1/2- state in quenched QCD. Results are obtained using a non-perturbatively O(a)-improved clover fermion action, and a splitting is found between the masses of the nucleon, and its parity partner. The calculation is performed on two lattice volumes and at three lattice spacings, enabling a study of both finite-volume and finite lattice-spacing uncertainties. A comparison is made with results obtained using the unimproved Wilson fermion action. (orig.)

  12. Variable elimination in chemical reaction networks with mass-action kinetics

    DEFF Research Database (Denmark)

    Feliu, Elisenda; Wiuf, C.

    2012-01-01

    We consider chemical reaction networks taken with mass-action kinetics. The steady states of such a system are solutions to a system of polynomial equations. Even for small systems the task of finding the solutions is daunting. We develop an algebraic framework and procedure for linear elimination...

  13. On the graph and systems analysis of reversible chemical reaction networks with mass action kinetics

    NARCIS (Netherlands)

    Rao, Shodhan; Jayawardhana, Bayu; Schaft, Arjan van der

    2012-01-01

    Motivated by the recent progresses on the interplay between the graph theory and systems theory, we revisit the analysis of reversible chemical reaction networks described by mass action kinetics by reformulating it using the graph knowledge of the underlying networks. Based on this formulation, we

  14. The Mass Spectrum of Light and Heavy Hadrons from Improved Lattice Actions

    OpenAIRE

    Ma, J. P.; McKellar, B. H. J.

    1997-01-01

    We use improved lattice actions for glue, light quarks and heavy quarks for which we use lattice NRQCD to compute hadron masses. Our results are in good agreement with experiment, except for charmed hadrons. It seems that charmed quar ks are not well approximated as heavy quarks nor as light quarks.

  15. E-FUSRAP: AUTOMATING THE CASE FILE FOR THE FORMERLY UTILIZED SITES REMEDIAL ACTION PROGRAM

    Energy Technology Data Exchange (ETDEWEB)

    Mackenzie, D.; Marshall, K.

    2003-02-27

    The Department of Energy's (DOE) Office of Site Closure, EM-30, houses the document library pertaining to sites that are related to the Formerly Utilized Sites Remedial Action Program (FUSRAP) and regularly addresses ongoing information demands, primarily from Freedom of Information Act (FOIA) requests, interested members of the public, the DOE, and other Federal Agencies. To address these demands more efficiently, DOE has begun to implement a new multi-phase, information management process known as e-FUSRAP. The first phase of e-FUSRAP, the development of the Considered Sites Database, summarizes and allows public access to complex information on over 600 sites considered as candidates for FUSRAP. The second phase of e-FUSRAP, the development of the Document Indexing Database, will create an internal index of more than 10,000 documents in the FUSRAP library's case file, allowing more effective management and retrieval of case file documents. Together, the phases of e-FUSRAP will allow EM-30 to become an innovative leader in enhancing public information sources.

  16. E-FUSRAP: AUTOMATING THE CASE FILE FOR THE FORMERLY UTILIZED SITES REMEDIAL ACTION PROGRAM

    International Nuclear Information System (INIS)

    The Department of Energy's (DOE) Office of Site Closure, EM-30, houses the document library pertaining to sites that are related to the Formerly Utilized Sites Remedial Action Program (FUSRAP) and regularly addresses ongoing information demands, primarily from Freedom of Information Act (FOIA) requests, interested members of the public, the DOE, and other Federal Agencies. To address these demands more efficiently, DOE has begun to implement a new multi-phase, information management process known as e-FUSRAP. The first phase of e-FUSRAP, the development of the Considered Sites Database, summarizes and allows public access to complex information on over 600 sites considered as candidates for FUSRAP. The second phase of e-FUSRAP, the development of the Document Indexing Database, will create an internal index of more than 10,000 documents in the FUSRAP library's case file, allowing more effective management and retrieval of case file documents. Together, the phases of e-FUSRAP will allow EM-30 to become an innovative leader in enhancing public information sources

  17. Optimizing TiO2-based phosphopeptide enrichment for automated multidimensional liquid chromatography coupled to tandem mass spectrometry

    OpenAIRE

    Cantin, Greg T.; Shock, Teresa R.; Park, Sung Kyu; Madhani, Hiten D.; Yates, John R.

    2007-01-01

    An automated online multidimensional liquid chromatography system coupled to ESI-based tandem mass spectrometry was used to assess the effectiveness of TiO2 in the enrichment of phosphopeptides from tryptic digests of protein mixtures. By monitoring the enrichment of phosphopeptides, an optimized set of loading, wash, and elution conditions were realized for TiO2. A comparison of TiO2 with other resins used for phosphopeptide enrichment, Fe(III)-IMAC and ZrO2, was also carried out using trypt...

  18. Micronucleus test for radiation biodosimetry in mass casualty events: Evaluation of visual and automated scoring

    International Nuclear Information System (INIS)

    In the case of a large-scale nuclear or radiological incidents a reliable estimate of dose is an essential tool for providing timely assessment of radiation exposure and for making life-saving medical decisions. Cytogenetics is considered as the 'gold standard' for biodosimetry. The dicentric analysis (DA) represents the most specific cytogenetic bioassay. The micronucleus test (MN) applied in interphase in peripheral lymphocytes is an alternative and simpler approach. A dose-effect calibration curve for the MN frequency in peripheral lymphocytes from 27 adult donors was established after in vitro irradiation at a dose range 0.15-8 Gy of 137Cs gamma rays (dose rate 6 Gy min-1). Dose prediction by visual scoring in a dose-blinded study (0.15-4.0 Gy) revealed a high level of accuracy (R = 0.89). The scoring of MN is time consuming and requires adequate skills and expertise. Automated image analysis is a feasible approach allowing to reduce the time and to increase the accuracy of the dose estimation decreasing the variability due to subjective evaluation. A good correlation (R = 0.705) between visual and automated scoring with visual correction was observed over the dose range 0-2 Gy. Almost perfect discrimination power for exposure to 1-2 Gy, and a satisfactory power for 0.6 Gy were detected. This threshold level can be considered sufficient for identification of sub lethally exposed individuals by automated CBMN assay.

  19. Micronucleus test for radiation biodosimetry in mass casualty events: Evaluation of visual and automated scoring

    Energy Technology Data Exchange (ETDEWEB)

    Bolognesi, Claudia, E-mail: claudia.bolognesi@istge.i [Environmental Carcinogenesis Unit, National Cancer Research Institute, Largo R. Benzi 10, 16132 Genoa (Italy); Balia, Cristina; Roggieri, Paola [Environmental Carcinogenesis Unit, National Cancer Research Institute, Largo R. Benzi 10, 16132 Genoa (Italy); Cardinale, Francesco [Clinical Epidemiology Unit, National Cancer Research Institute, Largo R. Benzi 10, 16132 Genoa (Italy); Department of Health Sciences, University of Genoa, Genoa (Italy); Bruzzi, Paolo [Clinical Epidemiology Unit, National Cancer Research Institute, Largo R. Benzi 10, 16132 Genoa (Italy); Sorcinelli, Francesca [Environmental Carcinogenesis Unit, National Cancer Research Institute, Largo R. Benzi 10, 16132 Genoa (Italy); Laboratory of Genetics, Histology and Molecular Biology Section, Army Medical and Veterinary, Research Center, Via Santo Stefano Rotondo 4, 00184 Roma (Italy); Lista, Florigio [Laboratory of Genetics, Histology and Molecular Biology Section, Army Medical and Veterinary, Research Center, Via Santo Stefano Rotondo 4, 00184 Roma (Italy); D' Amelio, Raffaele [Sapienza, Universita di Roma II Facolta di Medicina e Chirurgia and Ministero della Difesa, Direzione Generale Sanita Militare (Italy); Righi, Enzo [Frascati National Laboratories, National Institute of Nuclear Physics, Via Enrico Fermi 40, 00044 Frascati, Rome (Italy)

    2011-02-15

    In the case of a large-scale nuclear or radiological incidents a reliable estimate of dose is an essential tool for providing timely assessment of radiation exposure and for making life-saving medical decisions. Cytogenetics is considered as the 'gold standard' for biodosimetry. The dicentric analysis (DA) represents the most specific cytogenetic bioassay. The micronucleus test (MN) applied in interphase in peripheral lymphocytes is an alternative and simpler approach. A dose-effect calibration curve for the MN frequency in peripheral lymphocytes from 27 adult donors was established after in vitro irradiation at a dose range 0.15-8 Gy of {sup 137}Cs gamma rays (dose rate 6 Gy min{sup -1}). Dose prediction by visual scoring in a dose-blinded study (0.15-4.0 Gy) revealed a high level of accuracy (R = 0.89). The scoring of MN is time consuming and requires adequate skills and expertise. Automated image analysis is a feasible approach allowing to reduce the time and to increase the accuracy of the dose estimation decreasing the variability due to subjective evaluation. A good correlation (R = 0.705) between visual and automated scoring with visual correction was observed over the dose range 0-2 Gy. Almost perfect discrimination power for exposure to 1-2 Gy, and a satisfactory power for 0.6 Gy were detected. This threshold level can be considered sufficient for identification of sub lethally exposed individuals by automated CBMN assay.

  20. A microchip electrophoresis-mass spectrometric platform with double cell lysis nano-electrodes for automated single cell analysis.

    Science.gov (United States)

    Li, Xiangtang; Zhao, Shulin; Hu, Hankun; Liu, Yi-Ming

    2016-06-17

    Capillary electrophoresis-based single cell analysis has become an essential approach in researches at the cellular level. However, automation of single cell analysis has been a challenge due to the difficulty to control the number of cells injected and the irreproducibility associated with cell aggregation. Herein we report the development of a new microfluidic platform deploying the double nano-electrode cell lysis technique for automated analysis of single cells with mass spectrometric detection. The proposed microfluidic chip features integration of a cell-sized high voltage zone for quick single cell lysis, a microfluidic channel for electrophoretic separation, and a nanoelectrospray emitter for ionization in MS detection. Built upon this platform, a microchip electrophoresis-mass spectrometric method (MCE-MS) has been developed for automated single cell analysis. In the method, cell introduction, cell lysis, and MCE-MS separation are computer controlled and integrated as a cycle into consecutive assays. Analysis of large numbers of individual PC-12 neuronal cells (both intact and exposed to 25mM KCl) was carried out to determine intracellular levels of dopamine (DA) and glutamic acid (Glu). It was found that DA content in PC-12 cells was higher than Glu content, and both varied from cell to cell. The ratio of intracellular DA to Glu was 4.20±0.8 (n=150). Interestingly, the ratio drastically decreased to 0.38±0.20 (n=150) after the cells are exposed to 25mM KCl for 8min, suggesting the cells released DA promptly and heavily while they released Glu at a much slower pace in response to KCl-induced depolarization. These results indicate that the proposed MCE-MS analytical platform may have a great potential in researches at the cellular level. PMID:27207575

  1. Systematic forensic toxicological analysis by GC-MS in serum using automated mass spectral deconvolution and identification system.

    Science.gov (United States)

    Grapp, Marcel; Maurer, Hans H; Desel, Herbert

    2016-08-01

    Non-targeted screening of body fluids for psychoactive agents is an essential task for forensic toxicology. The challenge is the identification of xenobiotics of interest from background noise and endogenous matrix components. The aim of the present work was to evaluate the use of an Automated Mass Spectral Deconvolution and Identification System (AMDIS) for gas chromatography-mass spectrometry (GC-MS) based toxicological serum screening. One hundred fifty serum samples submitted to the authors´ laboratory for systematic forensic toxicological analysis underwent GC-MS screening after neutral and basic liquid-liquid extraction. Recorded datasets were routinely evaluated both by experienced personnel and automatically using the AMDIS software combined with the Maurer/Pfleger/Weber GC-MS library MPW_2011. The results from manual and automated data evaluation were then systematically compared. AMDIS parameters for data deconvolution and substance identification had been successfully adapted to the GC-MS screening procedure in serum. The number of false positive hits could substantially be reduced without increasing the risk of overlooking relevant compounds. With AMDIS-based data evaluation, additional drugs were identified in 25 samples (17%) that had not been detected by manual data evaluation. Importantly, among these drugs, there were frequently prescribed and toxicologically relevant antidepressants and antipsychotic drugs such as citalopram, mirtazapine, quetiapine, or venlafaxine. For most of the identified drugs, their serum concentrations were in the therapeutic or subtherapeutic range. Thus, our study indicated that automated data evaluation by AMDIS provided reliable screening results. Copyright © 2015 John Wiley & Sons, Ltd. PMID:26333204

  2. Sequential automated fusion/extraction chromatography methodology for the dissolution of uranium in environmental samples for mass spectrometric determination

    International Nuclear Information System (INIS)

    An improved methodology has been developed, based on dissolution by automated fusion followed by extraction chromatography for the detection and quantification of uranium in environmental matrices by mass spectrometry. A rapid fusion protocol (2/LiBr melts were used. The use of a M4 fusion unit also improved repeatability in sample preparation over muffle furnace fusion. Instrumental issues originating from the presence of high salt concentrations in the digestate after lithium metaborate fusion was also mitigated using an extraction chromatography (EXC) protocol aimed at removing lithium and interfering matrix constituants prior to the elution of uranium. The sequential methodology, which can be performed simultaneously on three samples, requires less than 20 min per sample for fusion and separation. It was successfully coupled to inductively coupled plasma mass spectrometry (ICP-MS) achieving detection limits below 100 pg kg-1 for 5-300 mg of sample.

  3. Estimation of heterogeneity in malaria transmission by stochastic modelling of apparent deviations from mass action kinetics

    Directory of Open Access Journals (Sweden)

    Smith Thomas A

    2008-01-01

    Full Text Available Abstract Background Quantifying heterogeneity in malaria transmission is a prerequisite for accurate predictive mathematical models, but the variance in field measurements of exposure overestimates true micro-heterogeneity because it is inflated to an uncertain extent by sampling variation. Descriptions of field data also suggest that the rate of Plasmodium falciparum infection is not proportional to the intensity of challenge by infectious vectors. This appears to violate the principle of mass action that is implied by malaria biology. Micro-heterogeneity may be the reason for this anomaly. It is proposed that the level of micro-heterogeneity can be estimated from statistical models that estimate the amount of variation in transmission most compatible with a mass-action model for the relationship of infection to exposure. Methods The relationship between the entomological inoculation rate (EIR for falciparum malaria and infection risk was reanalysed using published data for cohorts of children in Saradidi (western Kenya. Infection risk was treated as binomially distributed, and measurement-error (Poisson and negative binomial models were considered for the EIR. Models were fitted using Bayesian Markov chain Monte Carlo algorithms and model fit compared for models that assume either mass-action kinetics, facilitation, competition or saturation of the infection process with increasing EIR. Results The proportion of inocula that resulted in infection in Saradidi was inversely related to the measured intensity of challenge. Models of facilitation showed, therefore, a poor fit to the data. When sampling error in the EIR was neglected, either competition or saturation needed to be incorporated in the model in order to give a good fit. Negative binomial models for the error in exposure could achieve a comparable fit while incorporating the more parsimonious and biologically plausible mass action assumption. Models that assume negative binomial micro

  4. Modeling the Mass Action Dynamics of Metabolism with Fluctuation Theorems and Maximum Entropy

    Science.gov (United States)

    Cannon, William; Thomas, Dennis; Baxter, Douglas; Zucker, Jeremy; Goh, Garrett

    The laws of thermodynamics dictate the behavior of biotic and abiotic systems. Simulation methods based on statistical thermodynamics can provide a fundamental understanding of how biological systems function and are coupled to their environment. While mass action kinetic simulations are based on solving ordinary differential equations using rate parameters, analogous thermodynamic simulations of mass action dynamics are based on modeling states using chemical potentials. The latter have the advantage that standard free energies of formation/reaction and metabolite levels are much easier to determine than rate parameters, allowing one to model across a large range of scales. Bridging theory and experiment, statistical thermodynamics simulations allow us to both predict activities of metabolites and enzymes and use experimental measurements of metabolites and proteins as input data. Even if metabolite levels are not available experimentally, it is shown that a maximum entropy assumption is quite reasonable and in many cases results in both the most energetically efficient process and the highest material flux.

  5. Applicability of law of mass action to distribution of manganese between slag melts and liquid iron

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    According to the law of mass action and the coexistence theory of slag structure, the distribution of man ganese between MnO-FeO-SiO2 and MgO-MnO-FeO-SiO2 slag melts as well as liquid iron was analyzed. It is shown that K ′Mn and K Mno are only dependent on temperature and don' t change with basicities and compositions of slag melts. So the distribution of manganese between the above mentioned slag melts and molten iron obeys the law of mass action. But anal ysis of experimental results from other sources shows that K′Mn and K Mno really change with basicities of slag, which is probably arisen from not approaching equilibrium under low basicity slag melts.

  6. Applicability of mass action law to sulphur distribution between slag melts and liquid iron

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    According to the mass action law and the coexistence theory of slag structure, the calculating models of mass action concentration for CaO-MgO-FeO-Fe2O3-SiO2, CaO-MgO-MnO-FeO-Fe2O3-P2O5-SiO2 and CaO-MgO-MnO-FeO-Fe2O3-Al2O3-P2O5-SiO2 slag melts are formulated and sulphur distribution between the slag melts and liquid iron is treated. It is found that CaO, MnO and FeO promote desulphurization, while MgO is detrimental to desulphurization. In addition, the sulphur distribution coefficients between the slag melts and liquid iron are presented.

  7. An Automated High Performance Capillary Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometer for High-Throughput Proteomics

    International Nuclear Information System (INIS)

    We report on a fully automated 9.4 tesla Fourier transform ion resonance cyclotron (FTICR) mass spectrometer coupled to reverse-phase chromatography for high-throughput proteomic studies. Modifications made to the front-end of a commercial FTICR instrument--a dual-ESI-emitter ion source; dual-channel electrodynamic ion funnel; and collisional-cooling, selection and accumulation quadrupoles--significantly improved the sensitivity, dynamic range and mass measurement accuracy of the mass spectrometer. A high-pressure capillary liquid chromatography (LC) system was incorporated with an autosampler that enabled 24 h/day operation. A novel method for accumulating ions in the ICR cell was also developed. Unattended operation of the instrument revealed the exceptional reproducibility (1-5% deviation in elution times for peptides from a bacterial proteome), repeatability (10-20% deviation in detected abundances for peptides from the same aliquot analyzed a few weeks apart) and robustness (high-throughput operation for 5 months without downtime) of the LC/FTICR system. When combined with modulated-ion-energy gated trapping, the internal calibration of FTICR mass spectra decreased dispersion of mass measurement errors for peptide identifications in conjunction with high resolution capillary LC separations to < 5 ppm over a dynamic range for each spectrum of 10 3

  8. Anomalous reaction-transport processes: the dynamics beyond the Mass Action Law

    OpenAIRE

    Campos Moreno, Daniel; Fedotov, Sergei; Méndez López, Vicenç

    2008-01-01

    In this paper we reconsider the Mass Action Law (MAL) for the anomalous reversible reaction $A\\rightleftarrows B$ with diffusion. We provide a mesoscopic description of this reaction when the transitions between two states $A$ and $B$ are governed by anomalous (heavy-tailed) waiting-time distributions. We derive the set of mesoscopic integro-differential equations for the mean densities of reacting and diffusing particles in both states. We show that the effective reaction rate memory kernels...

  9. Calculating model of mass action concentrations for Ag-Au-Cu melts

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Based on phase diagrams and measured activities, the calculating model of mass action concentrations for heterogeneous melts Ag-Au-Cu was formulated. Calculated results agree with the improved results of recent research work.showing that the model formulated can reflect the structural characteristics of these melts. In this model, without the help of any empirical parameters, only three equilibrium constants are used, hence it is simple, lear, and favorable to the simplification of calculation.

  10. On the graph and systems analysis of reversible chemical reaction networks with mass action kinetics

    OpenAIRE

    Rao, Shodhan; Jayawardhana, Bayu; der Schaft, Arjan van

    2012-01-01

    Motivated by the recent progresses on the interplay between the graph theory and systems theory, we revisit the analysis of reversible chemical reaction networks described by mass action kinetics by reformulating it using the graph knowledge of the underlying networks. Based on this formulation, we can characterize the space of equilibrium points and provide simple dynamical analysis on the state space modulo the space of equilibrium points.

  11. On the Mathematical Structure of Balanced Chemical Reaction Networks Governed by Mass Action Kinetics

    OpenAIRE

    der Schaft, Arjan van; Rao, Shodhan; Jayawardhana, Bayu

    2011-01-01

    Motivated by recent progress on the interplay between graph theory, dynamics, and systems theory, we revisit the analysis of chemical reaction networks described by mass action kinetics. For reaction networks possessing a thermodynamic equilibrium we derive a compact formulation exhibiting at the same time the structure of the complex graph and the stoichiometry of the network, and which admits a direct thermodynamical interpretation. This formulation allows us to easily characterize the set ...

  12. Effects of mass action equilibria on fixed-bed multicomponent ion-exchange dynamics

    International Nuclear Information System (INIS)

    A generalized parallel pore and surface diffusion model and associated dynamic simulation program have been developed for multicomponent fixed-bed ion-exchange processes. Both equilibrium and nonequilibrium mass action laws are used to describe stoichiometric ion exchange. Model equations are solved numerically for frontal, pulse, or sequential loading processes. Analytical solutions obtained from a local equilibrium theory for binary systems and experimental data of two multicomponent systems served as benchmarks for the numerical solutions. The results indicate that the parallel pore and surface diffusion model should be considered for nonlinear large-particle systems. A parametric study shows that a major difference in fixed-bed dynamics between mass action and Langmuir systems lies in the propagation of diffuse waves of multivalent ions. Generally, the higher the valence or mass action equilibrium constant, the more pronounced the tailing of diffuse waves, which results in apparent adsorption hysteresis in a loading and washing cycle. The apparently irreversibly adsorbed multivalent ions can be eluted by concentrated solutions of lower valence ions, as a result of the relative selectivities of the higher valence against lower valence ions decreasing with increasing total solution phase concentration. This can lead to changes from favorable to unfavorable isotherms and self-sharpening eaves to diffuse waves, or vice versa. Other results show that elution order can be reversed for heterovalent ions in elution and displacement chromatography

  13. Automated solid-phase extraction of herbicides from water for gas chromatographic-mass spectrometric analysis

    Science.gov (United States)

    Meyer, M.T.; Mills, M.S.; Thurman, E.M.

    1993-01-01

    An automated solid-phase extraction (SPE) method was developed for the pre-concentration of chloroacetanilide and triazine herbicides, and two triazine metabolites from 100-ml water samples. Breakthrough experiments for the C18 SPE cartridge show that the two triazine metabolites are not fully retained and that increasing flow-rate decreases their retention. Standard curve r2 values of 0.998-1.000 for each compound were consistently obtained and a quantitation level of 0.05 ??g/l was achieved for each compound tested. More than 10,000 surface and ground water samples have been analyzed by this method.

  14. Towards Automation 2.0: A Neurocognitive Model for Environment Recognition, Decision-Making, and Action Execution

    OpenAIRE

    Zucker Gerhard; Dietrich Dietmar; Velik Rosemarie

    2011-01-01

    The ongoing penetration of building automation by information technology is by far not saturated. Today's systems need not only be reliable and fault tolerant, they also have to regard energy efficiency and flexibility in the overall consumption. Meeting the quality and comfort goals in building automation while at the same time optimizing towards energy, carbon footprint and cost-efficiency requires systems that are able to handle large amounts of information and negotiate system behaviour ...

  15. Automated DEM extraction in digital aerial photogrammetry: precisions and validation for mass movement monitoring

    Directory of Open Access Journals (Sweden)

    A. Pesci

    2005-06-01

    Full Text Available Automated procedures for photogrammetric image processing and Digital Elevation Models (DEM extraction yield high precision terrain models in a short time, reducing manual editing; their accuracy is strictly related to image quality and terrain features. After an analysis of the performance of the Digital Photogrammetric Workstation (DPW 770 Helava, the paper compares DEMs derived from different surveys and registered in the same reference system. In the case of stable area, the distribution of height residuals, their mean and standard deviation values, indicate that the theoretical accuracy is achievable automatically when terrain is characterized by regular morphology. Steep slopes, corrugated surfaces, vegetation and shadows can degrade results even if manual editing procedures are applied. The comparison of multi-temporal DEMs on unstable areas allows the monitoring of surface deformation and morphological changes.

  16. Pep2Path: automated mass spectrometry-guided genome mining of peptidic natural products

    NARCIS (Netherlands)

    Medema, Marnix; Paalvast, Yared; Nguyen, D.D.; Melnik, A.; Dorrestein, P.C.; Takano, Eriko; Breitling, Rainer

    2014-01-01

    Nonribosomally and ribosomally synthesized bioactive peptides constitute a source of molecules of great biomedical importance, including antibiotics such as penicillin, immunosuppressants such as cyclosporine, and cytostatics such as bleomycin. Recently, an innovative mass-spectrometry-based strateg

  17. Action-Type Variational Principles For Hyperbolic and Parabolic Heat & Mass Transfer

    Directory of Open Access Journals (Sweden)

    Stanislaw Sieniutycz

    2000-06-01

    Full Text Available For the field or Eulerian description of heat conduction, a method is discussed associated with description of thermal fields by a variational principle involving suitably constructed potentials rather than original physical variables. The considered processes are: simple hyperbolic heat transfer and coupled parabolic transfer of heat, mass and electric charge. By using various gradient or nongradient representations of original physical fields in terms of potentials, which are quantities of similar sort as those which Clebsch used in his representation of hydrodynamic velocity, suitable action-type criteria can be found, and corresponding Lagrangian and Hamiltonian formalisms can be developed. Symmetry principles can also be considered and components of energy-momentum tensor can be evaluated for a gauged lagrangian. The limiting reversible case appears as a suitable reference frame. The results suggest that thermodynamic irreversibility does not change neither kinetic potential nor action functional; it only complicates potential representations of physical fields in comparison with those describing the reversible evolution.

  18. Automated methods for quantitative and qualitative analysis of PTR-TOF mass spectra

    International Nuclear Information System (INIS)

    Statistical analysis of measured signals from counting systems is a common method to increase the accuracy and precision of peak position and peak area. The most common approach to analyze data gained from counting systems is to fit the data peak by peak using an appropriate probability density function (PDF) like a Gaussian function. Since a counting system creates histograms, the counted data do not represent data points of the anticipated PDF. Therefore, one should not fit any PDF directly to the histogram data. Here we present a solution to this problem by fitting distributions instead of densities. A simple formula allows to correct for Poisson statistics and dead-time effects. The improved peak analysis method is applied to mass spectra obtained from a recently developed proton-transfer-reaction time-offlight mass spectrometer (PTR-TOF) enhancing the mass accuracy and peak quantification. (author)

  19. An automated system for measuring the mass flowrate of powders in transport lines

    Science.gov (United States)

    Littman, Howard; Morgan, Morris B., III; Prapas, Demetrios K.; Rubel, Glen O.

    1990-08-01

    A new automated particle transport (APT) system has been developed for studying the dissemination of bulk powders into deagglomerated aerosols. It consists of a 1.12-inch ID transport line with a spout-fluidized bed feeder. The particles are transported from an aerated annulus into the transport line and collected in a closed can or bag filter. Two separate feed lines supply the air necessary to operate the transport line and aerate the particles in order that they flow smoothly into the transport line. An IBM PC AT computer clone equipped with a data translation DT 2806 multifunction input-output board and A to D and D to A modules (DTX 311 and 328) is used for both control and data acquisition. A fluid mechanical model of the flow has been developed and the APT system will be used to verify it. Experiments will be conducted to measure the choking velocity, drag coefficient, fluid and particle flowrates, and pressure distribution in the line.

  20. Automated Sample Preparation Platform for Mass Spectrometry-Based Plasma Proteomics and Biomarker Discovery

    Directory of Open Access Journals (Sweden)

    Vilém Guryča

    2014-03-01

    Full Text Available The identification of novel biomarkers from human plasma remains a critical need in order to develop and monitor drug therapies for nearly all disease areas. The discovery of novel plasma biomarkers is, however, significantly hampered by the complexity and dynamic range of proteins within plasma, as well as the inherent variability in composition from patient to patient. In addition, it is widely accepted that most soluble plasma biomarkers for diseases such as cancer will be represented by tissue leakage products, circulating in plasma at low levels. It is therefore necessary to find approaches with the prerequisite level of sensitivity in such a complex biological matrix. Strategies for fractionating the plasma proteome have been suggested, but improvements in sensitivity are often negated by the resultant process variability. Here we describe an approach using multidimensional chromatography and on-line protein derivatization, which allows for higher sensitivity, whilst minimizing the process variability. In order to evaluate this automated process fully, we demonstrate three levels of processing and compare sensitivity, throughput and reproducibility. We demonstrate that high sensitivity analysis of the human plasma proteome is possible down to the low ng/mL or even high pg/mL level with a high degree of technical reproducibility.

  1. Generalized mass action systems: Complex balancing equilibria and sign vectors of the stoichiometric and kinetic-order subspaces

    OpenAIRE

    Müller, Stefan; Regensburger, Georg

    2012-01-01

    Mass action systems capture chemical reaction networks in homogeneous and dilute solutions. We suggest a notion of generalized mass action systems that admits arbitrary nonnegative power-law rate functions and serves as a more realistic model for reaction networks in intracellular environments. In addition to the chemical complexes and the related stoichiometric subspace, we introduce corresponding kinetic complexes, which represent the nonnegative exponents in the rate functions and determin...

  2. Fully automated screening of veterinary drugs in milk by turbulent flow chromatography and tandem mass spectrometry

    NARCIS (Netherlands)

    Stolker, A.A.M.; Peters, R.J.B.; Zuiderent, R.; DiBussolo, J.M.; Martins, C.P.B.

    2010-01-01

    There is an increasing interest in screening methods for quick and sensitive analysis of various classes of veterinary drugs with limited sample pre-treatment. Turbulent flow chromatography in combination with tandem mass spectrometry has been applied for the first time as an efficient screening met

  3. Automated combustion accelerator mass spectrometry for the analysis of biomedical samples in the low attomole range

    NARCIS (Netherlands)

    Duijn, E. van; Sandman, H.; Grossouw, D.; Mocking, J.A.J.; Coulier, L.; Vaes, W.H.J.

    2014-01-01

    The increasing role of accelerator mass spectrometry (AMS) in biomedical research necessitates modernization of the traditional sample handling process. AMS was originally developed and used for carbon dating, therefore focusing on a very high precision but with a comparably low sample throughput. H

  4. Design and evaluation of a new automated method for the segmentation and characterization of masses on ultrasound images

    Science.gov (United States)

    Cui, Jing; Sahiner, Berkman; Chan, Heang-Ping; Nees, Alexis; Paramagul, Chintana; Hadjiiski, Lubomir M.; Zhou, Chuan; Shi, Jiazheng

    2008-03-01

    Segmentation of masses is the first step in most computer-aided diagnosis (CAD) systems for characterization of breast masses as malignant or benign. In this study, we designed an automated method for segmentation of masses on ultrasound (US) images. The method automatically estimated an initial contour based on a manually-identified point approximately at the mass center. A two-stage active contour (AC) method iteratively refined the initial contour and performed self-examination and correction on the segmentation result. To evaluate our method, we compared it with manual segmentation by an experienced radiologists (R1) on a data set of 226 US images containing biopsy-proven masses from 121 patients (44 malignant and 77 benign). Four performance measures were used to evaluate the segmentation accuracy; two measures were related to the overlap between the computer and radiologist segmentation, and two were related to the area difference between the two segmentation results. To compare the difference between the segmentation results by the computer and R1 to inter-observer variation, a second radiologist (R2) also manually segmented all masses. The two overlap measures between the segmentation results by the computer and R1 were 0.87+ 0.16 and 0.73+ 0.17 respectively, indicating a high agreement. However, the segmentation results between two radiologists were more consistent. To evaluate the effect of the segmentation method on classification accuracy, three feature spaces were formed by extracting texture, width-to-height, and posterior shadowing features using the computer segmentation, R1's manual segmentation, and R2's manual segmentation. A linear discriminant analysis classifier using stepwise feature selection was tested and trained by a leave-one-case-out method to characterize the masses as malignant or benign. For case-based classification, the area A z under the test receiver operating characteristic (ROC) curve was 0.90+/-0.03, 0.87+/-0.03 and 0

  5. Mass action realizations of reaction kinetic system models on various time scales

    Energy Technology Data Exchange (ETDEWEB)

    Hangos, K M; Szederkenyi, G, E-mail: hangos@scl.sztaki.hu, E-mail: szeder@scl.sztaki.hu [Process Control Research Group, Computer and Automation Reseach Institute, Kende u. 13-17, H-1111 Budapest (Hungary)

    2011-01-01

    Complex chemical reaction networks often exhibit different dynamic behaviour on different time scales. A combined approach is proposed in this work for determining physically meaningful mass action realizations of complex chemical reaction networks that describe its dynamic behaviour on different time scales. This is achieved by appropriately reducing the detailed overall mass action kinetic scheme using quasi steady state assumptions fit to the particular time scale, and then searching for an optimal realization using mixed integer linear programing. Furthermore, the relationship between the properties (reversibility, deficiency, stability) of the obtained realizations of the same system on different time scales are also investigated and related to the same properties of the detailed overall model. It is shown that the reduced models obtained by quasi steady state assumptions may show exotic nonlinear behaviour, such as oscillations, when the original detailed is globally asymptotically stable. The proposed methods are illustrated by using a simple Michaelis-Menten type reaction kinetic example. The simplified versions of the well known Brusselator model have also been investigated and presented as a case study.

  6. Facile method for automated genotyping of single nucleotide polymorphisms by mass spectrometry

    OpenAIRE

    Sauer, Sascha; Gelfand, David H.; Boussicault, Francis; Bauer, Keith; Reichert, Fred; Gut, Ivo G.

    2002-01-01

    In the future, analysis of single nucleotide polymorphisms (SNPs) should become a powerful tool for many genetic applications in areas such as association studies, pharmacogenetics and traceability in the agro-alimentary sector. A number of technologies have been developed for high-throughput genotyping of SNPs. Here we present the simplified GOOD assay for SNP genotyping by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI). The simplified GOOD assay is a si...

  7. Mass asymmetry and tricyclic wobble motion assessment using automated launch video analysis

    Directory of Open Access Journals (Sweden)

    Ryan Decker

    2016-04-01

    Examination of the pitch and yaw histories clearly indicates that in addition to epicyclic motion's nutation and precession oscillations, an even faster wobble amplitude is present during each spin revolution, even though some of the amplitudes of the oscillation are smaller than 0.02 degree. The results are compared to a sequence of shots where little appreciable mass asymmetries were present, and only nutation and precession frequencies are predominantly apparent in the motion history results. Magnitudes of the wobble motion are estimated and compared to product of inertia measurements of the asymmetric projectiles.

  8. Computer-aided detection system for masses in automated whole breast ultrasonography: development and evaluation of the effectiveness

    International Nuclear Information System (INIS)

    The aim of this study was to evaluate the performance of a proposed computer-aided detection (CAD) system in automated breast ultrasonography (ABUS). Eighty-nine two-dimensional images (20 cysts, 42 benign lesions, and 27 malignant lesions) were obtained from 47 patients who underwent ABUS (ACUSON S2000). After boundary detection and removal, we detected mass candidates by using the proposed adjusted Otsu's threshold; the threshold was adaptive to the variations of pixel intensities in an image. Then, the detected candidates were segmented. Features of the segmented objects were extracted and used for training/testing in the classification. In our study, a support vector machine classifier was adopted. Eighteen features were used to determine whether the candidates were true lesions or not. A five-fold cross validation was repeated 20 times for the performance evaluation. The sensitivity and the false positive rate per image were calculated, and the classification accuracy was evaluated for each feature. In the classification step, the sensitivity of the proposed CAD system was 82.67% (SD, 0.02%). The false positive rate was 0.26 per image. In the detection/segmentation step, the sensitivities for benign and malignant mass detection were 90.47% (38/42) and 92.59% (25/27), respectively. In the five-fold cross-validation, the standard deviation of pixel intensities for the mass candidates was the most frequently selected feature, followed by the vertical position of the centroids. In the univariate analysis, each feature had 50% or higher accuracy. The proposed CAD system can be used for lesion detection in ABUS and may be useful in improving the screening efficiency.

  9. Facile method for automated genotyping of single nucleotide polymorphisms by mass spectrometry.

    Science.gov (United States)

    Sauer, Sascha; Gelfand, David H; Boussicault, Francis; Bauer, Keith; Reichert, Fred; Gut, Ivo G

    2002-03-01

    In the future, analysis of single nucleotide polymorphisms (SNPs) should become a powerful tool for many genetic applications in areas such as association studies, pharmacogenetics and traceability in the agro-alimentary sector. A number of technologies have been developed for high-throughput genotyping of SNPs. Here we present the simplified GOOD assay for SNP genotyping by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI). The simplified GOOD assay is a single-tube, purification-free, three-step procedure consisting of PCR, primer extension and phosphodiesterase II digestion followed by mass spectrometric analysis. Due to the application of charge-tag technology, no sample purification is required prior to the otherwise very impurity-sensitive MALDI analysis. The use of methylphosphonate containing primers and ddNTPs or alpha-S-ddNTPs together with a novel DNA polymerase derived from Thermotoga maritima for primer extension allow the fluent preparation of negatively charge-tagged, allele-specific products. A key feature of this polymerase is its preference for ddNTPs and alpha-S-ddNTPs over dNTPs. The simplified GOOD assay was run with automatic liquid handling at the lowest manageable volumes, automatic data acquisition and interpretation. We applied this novel procedure to genotyping SNPs of candidate genes for hypertension and cardiovascular disease. PMID:11861927

  10. Design and performance of an instrument for electron impact tandem mass spectrometry and action spectroscopy of mass/charge selected macromolecular ions stored in RF ion trap*

    Science.gov (United States)

    Ranković, Milos Lj.; Giuliani, Alexandre; Milosavljević, Aleksandar R.

    2016-06-01

    A new apparatus was designed, coupling an electron gun with a linear quadrupole ion trap mass spectrometer, to perform m/ z (mass over charge) selected ion activation by electron impact for tandem mass spectrometry and action spectroscopy. We present in detail electron tracing simulations of a 300 eV electron beam inside the ion trap, design of the mechanical parts, electron optics and electronic circuits used in the experiment. We also report examples of electron impact activation tandem mass spectra for Ubiquitin protein, Substance P and Melittin peptides, at incident electron energies in the range from 280 eV to 300 eV.

  11. Investigating the Kinematics of Coronal Mass Ejections with the Automated CORIMP Catalog

    CERN Document Server

    Byrne, Jason P

    2015-01-01

    Studying coronal mass ejections (CMEs) in coronagraph data can be challenging due to their diffuse structure and transient nature, compounded by the variations in their dynamics, morphology, and frequency of occurrence. The large amounts of data available from missions like the Solar and Heliospheric Observatory (SOHO) make manual cataloging of CMEs tedious and prone to human error, and so a robust method of detection and analysis is required and often preferred. A new coronal image processing catalog called CORIMP has been developed in an effort to achieve this, through the implementation of a dynamic background separation technique and multiscale edge detection. These algorithms together isolate and characterise CME structure in the field-of-view of the Large Angle Spectrometric Coronagraph (LASCO) onboard SOHO. CORIMP also applies a Savitzky-Golay filter, along with quadratic and linear fits, to the height-time measurements for better revealing the true CME speed and acceleration profiles across the plane-...

  12. Integration of biotechnology, visualisation technology and robot technology for automated mass propagation af elite trees

    DEFF Research Database (Denmark)

    Find, Jens

    stages (Fig. 1). Improved methods must be developed for transfer and acclimatisation of plants from sterile in vitro conditions to non sterile (ex vitro/in vivo) conditions at the nursery. Fig. 1. Predicted labour cost by clonal propagation through somatic embryogenesis. The labour costs are very low in......Somatic embryogenesis (SE) is one of the promisisng methods for integration of biotechnology into breeding and propagation of conifers. Especially because SE has some particular advantages for the development of cost effective methods for clonal mass propagation of elite trees: It is a very...... effective and fast method for clonal propagation. The method is suitable for automatisation and robot technology. The method is, for several plant species, the preferred basis for development of additional biotechnological breeding technologies as e.g. genetic transformation. Elite clones can be stored over...

  13. Nonequilibrium thermodynamic formalism of nonlinear chemical reaction systems with Waage-Guldberg's law of mass action

    Science.gov (United States)

    Ge, Hao; Qian, Hong

    2016-06-01

    Macroscopic entropy production rate σ (tot) in the general nonlinear isothermal chemical reaction system with mass action kinetics is decomposed into a free energy dissipation rate and a house-keeping heat dissipation rate: σ (tot) =σ (fd) +σ (hk) ; σ (fd) = -d A /d t , where A is a generalized free energy function. This yields a novel nonequilibrium free energy balance equation d A /d t = -σ (tot) +σ (hk) , which is on a par with celebrated entropy balance equation d S /d t =σ (tot) +η (ex) where η (ex) is the rate of entropy exchange with the environment. For kinetic systems with complex balance, σ (fd) and σ (hk) are the macroscopic limits of stochastic free energy dissipation rate and house-keeping heat dissipation rate, which are both nonnegative, in the Delbrück-Gillespie description of the stochastic chemical kinetics. A full kinetic and thermodynamic theory of chemical reaction systems that transcends mesoscopic and macroscopic levels emerges.

  14. An atomistic vision of the Mass Action Law: Prediction of carbon/oxygen defects in silicon

    International Nuclear Information System (INIS)

    We introduce an atomistic description of the kinetic Mass Action Law to predict concentrations of defects and complexes. We demonstrate in this paper that this approach accurately predicts carbon/oxygen related defect concentrations in silicon upon annealing. The model requires binding and migration energies of the impurities and complexes, here obtained from density functional theory (DFT) calculations. Vacancy-oxygen complex kinetics are studied as a model system during both isochronal and isothermal annealing. Results are in good agreement with experimental data, confirming the success of the methodology. More importantly, it gives access to the sequence of chain reactions by which oxygen and carbon related complexes are created in silicon. Beside the case of silicon, the understanding of such intricate reactions is a key to develop point defect engineering strategies to control defects and thus semiconductors properties

  15. An atomistic vision of the Mass Action Law: Prediction of carbon/oxygen defects in silicon

    Energy Technology Data Exchange (ETDEWEB)

    Brenet, G.; Timerkaeva, D.; Caliste, D.; Pochet, P. [CEA, INAC-SP2M, Atomistic Simulation Laboratory, F-38000 Grenoble (France); Univ. Grenoble Alpes, INAC-SP2M, L-Sim, F-38000 Grenoble (France); Sgourou, E. N.; Londos, C. A. [University of Athens, Solid State Physics Section, Panepistimiopolis Zografos, Athens 157 84 (Greece)

    2015-09-28

    We introduce an atomistic description of the kinetic Mass Action Law to predict concentrations of defects and complexes. We demonstrate in this paper that this approach accurately predicts carbon/oxygen related defect concentrations in silicon upon annealing. The model requires binding and migration energies of the impurities and complexes, here obtained from density functional theory (DFT) calculations. Vacancy-oxygen complex kinetics are studied as a model system during both isochronal and isothermal annealing. Results are in good agreement with experimental data, confirming the success of the methodology. More importantly, it gives access to the sequence of chain reactions by which oxygen and carbon related complexes are created in silicon. Beside the case of silicon, the understanding of such intricate reactions is a key to develop point defect engineering strategies to control defects and thus semiconductors properties.

  16. Propagation of fluctuations in interaction networks governed by the law of mass action

    CERN Document Server

    Maslov, Sergei; Ispolatov, Iaroslav

    2007-01-01

    Using an example of physical interactions between proteins, we study how perturbations propagate in interconnected networks whose equilibrium state is governed by the law of mass action. We introduce a comprehensive matrix formalism which predicts the response of this equilibrium to small changes in total concentrations of individual molecules, and explain it using a heuristic analogy to a current flow in a network of resistors. Our main conclusion is that on average changes in free concentrations exponentially decay with the distance from the source of perturbation. We then study how this decay is influenced by such factors as the topology of a network, binding strength, and correlations between concentrations of neighboring nodes. An exact analytic expression for the decay constant is obtained for the case of uniform interactions on the Bethe lattice. Our general findings are illustrated using a real biological network of protein-protein interactions in baker's yeast with experimentally determined protein c...

  17. Monte Carlo simulation of the transmission of measles: Beyond the mass action principle

    Science.gov (United States)

    Zekri, Nouredine; Clerc, Jean Pierre

    2002-04-01

    We present a Monte Carlo simulation of the transmission of measles within a population sample during its growing and equilibrium states by introducing two different vaccination schedules of one and two doses. We study the effects of the contact rate per unit time ξ as well as the initial conditions on the persistence of the disease. We found a weak effect of the initial conditions while the disease persists when ξ lies in the range 1/L-10/L (L being the latent period). Further comparison with existing data, prediction of future epidemics and other estimations of the vaccination efficiency are provided. Finally, we compare our approach to the models using the mass action principle in the first and another epidemic region and found the incidence independent of the number of susceptibles after the epidemic peak while it strongly fluctuates in its growing region. This method can be easily applied to other human, animal, and plant diseases and includes more complicated parameters.

  18. Preclusion of switch behavior in reaction networks with mass-action kinetics

    DEFF Research Database (Denmark)

    Feliu, Elisenda; Wiuf, C.

    2012-01-01

    We study networks taken with mass-action kinetics and provide a Jacobian criterion that applies to an arbitrary network to preclude the existence of multiple positive steady states within any stoichiometric class for any choice of rate constants. We are concerned with the characterization of...... injective networks, that is, networks for which the species formation rate function is injective in the interior of the positive orthant within each stoichiometric class. We show that a network is injective if and only if the determinant of the Jacobian of a certain function does not vanish. The function...... precludes the existence of degenerate steady states. Further, we relate injectivity of a network to that of the network obtained by adding outflow, or degradation, reactions for all species....

  19. Monte Carlo simulation of the transmission of measles: Beyond the mass action principle

    International Nuclear Information System (INIS)

    We present a Monte Carlo simulation of the transmission of measles within a population sample during its growing and equilibrium states by introducing two different vaccination schedules of one and two doses. We study the effects of the contact rate per unit time ξ as well as the initial conditions on the persistence of the disease. We found a weak effect of the initial conditions while the disease persists when ξ lies in the range 1/L-10/L (L being the latent period). Further comparison with existing data, prediction of future epidemics and other estimations of the vaccination efficiency are provided. Finally, we compare our approach to the models using the mass action principle in the first and another epidemic region and found the incidence independent of the number of susceptibles after the epidemic peak while it strongly fluctuates in its growing region. This method can be easily applied to other human, animal, and plant diseases and includes more complicated parameters

  20. ["Mobilize the masses to urgently go into action and overcome the drought"].

    Science.gov (United States)

    1981-08-14

    At present, the drought is serious. Urgently taking action and doing everything possible to overcome the drought and strive for a bumper autumn harvest is an extremely urgent combat task facing the people of our province. Since mid-June, it has rained in our province several times but it has not rained very evenly. It has not rained in many places during the past 1/2 year and more. By the end of July, autumn crops on 37.35 million mu of land have been affected by the drought, accounting for more than 1/3 of the total area of the autumn crops. The drought has greatly harmed the growth of autumn crops. If we do not act now the drought will seriously affect the autumn harvest. Mobilizing the masses to urgently go into action to overcome the drought and strive for a bumper autumn harvest is a major task which has a bearing on the livelihood of both the urban and rural people of our province and on the further development of the excellent situation. Therefore, party committees at all levels must pay attention to this, concentrate the forces to grasp it firmly and well, and not lower their guard. The party members, cadres, and masses must carry forward the spirit of fighting continuously and struggling hard, make full use of all water conservancy projects and antidrought machinery and tools, try their best to find new water resources, and use water reasonably and sparingly. All walks of life and trades must do everything possible to vigorously support the struggle against the drought and do well in supplying the oil and electricity needed for agricultural machinery and help to repair machinery and tools. We all must contribute toward the effort to achieve a bumper autumn harvest. PMID:12338066

  1. Hybrid Markov-mass action law for cell activation by rare binding events

    CERN Document Server

    Holcman, C Guerrier D

    2016-01-01

    The binding of molecules, ions or proteins to specific target sites is a generic step for cell activation. However, this step relies on rare events where stochastic particles located in a large bulk are searching for small and often hidden targets and thus remains difficult to study. We present here a hybrid discrete-continuum model where the large ensemble of particles is described by mass-action laws. The rare discrete binding events are modeled by a Markov chain for the encounter of a finite number of small targets by few Brownian particles, for which the arrival time is Poissonian. This model is applied for predicting the time distribution of vesicular release at neuronal synapses that remains elusive. This release is triggered by the binding of few calcium ions that can originate either from the synaptic bulk or from the transient entry through calcium channels. We report that the distribution of release time is bimodal although triggered by a single fast action potential: while the first peak follows a ...

  2. Gas chromatographic mass analysis and further pharmacological actions of Cymbopogon proximus essential oil.

    Science.gov (United States)

    Al-Taweel, A M; Fawzy, G A; Perveen, S; El Tahir, K E H

    2013-09-01

    The present study reports Gas chromatographic mass analysis (GC-MS) as well as important biological activities of Cymbopogon proximus essential oil. The chemical composition of the essential oil of Cymbopogon proximus was investigated by GC-MS. Furthermore, the effects of Cymbopogon proximus essential oil on the cardiac parasympathetic ganglia in rats, the intra-tracheal pressure in guinea-pigs and on carrageenan-induced inflammation in the rats paw, were studied. The GC-MS study led to the identification of 22 components with Piperitone representing (73.81%), Elemol (9.32%), alpha-Eudesmol (5.21%) and alpha-Terpineol (3.01%) of the oils composition. The percentage protective effect of the oil on the vagus-induced bradycardia in rats was 90.1±3.1%, which represents a significant protection. As for the effect of Cymbopogon oil on bronchoconstrictors-induced increase in intra-tracheal pressure in guinea-pigs, the oil antagonized the actions of 5-HT and histamine by 80±3.7 and 93±8.3%, respectively. Pharmacological investigations using Cymbopogon oil revealed its inherent ability to possess a bronchodilator activity mediated via blockade of both histamine and serotonin receptors. It possessed a significant ganglionic blocking action and a limited anti-inflammatory activity that seemed to involve blockade of histamine and serotonin receptors in the rats' paws. PMID:23780497

  3. DetectTLC: Automated Reaction Mixture Screening Utilizing Quantitative Mass Spectrometry Image Feature

    Science.gov (United States)

    Kaddi, Chanchala D.; Bennett, Rachel V.; Paine, Martin R. L.; Banks, Mitchel D.; Weber, Arthur L.; Fernández, Facundo M.; Wang, May D.

    2016-01-01

    Full characterization of complex reaction mixtures is necessary to understand mechanisms, optimize yields, and elucidate secondary reaction pathways. Molecular-level information for species in such mixtures can be readily obtained by coupling mass spectrometry imaging (MSI) with thin layer chromatography (TLC) separations. User-guided investigation of imaging data for mixture components with known m/z values is generally straightforward; however, spot detection for unknowns is highly tedious, and limits the applicability of MSI in conjunction with TLC. To accelerate imaging data mining, we developed DetectTLC, an approach that automatically identifies m/z values exhibiting TLC spot-like regions in MS molecular images. Furthermore, DetectTLC can also spatially match m/z values for spots acquired during alternating high and low collision-energy scans, pairing product ions with precursors to enhance structural identification. As an example, DetectTLC is applied to the identification and structural confirmation of unknown, yet significant, products of abiotic pyrazinone and aminopyrazine nucleoside analog synthesis. PMID:26508443

  4. DetectTLC: Automated Reaction Mixture Screening Utilizing Quantitative Mass Spectrometry Image Features

    Science.gov (United States)

    Kaddi, Chanchala D.; Bennett, Rachel V.; Paine, Martin R. L.; Banks, Mitchel D.; Weber, Arthur L.; Fernández, Facundo M.; Wang, May D.

    2016-02-01

    Full characterization of complex reaction mixtures is necessary to understand mechanisms, optimize yields, and elucidate secondary reaction pathways. Molecular-level information for species in such mixtures can be readily obtained by coupling mass spectrometry imaging (MSI) with thin layer chromatography (TLC) separations. User-guided investigation of imaging data for mixture components with known m/z values is generally straightforward; however, spot detection for unknowns is highly tedious, and limits the applicability of MSI in conjunction with TLC. To accelerate imaging data mining, we developed DetectTLC, an approach that automatically identifies m/z values exhibiting TLC spot-like regions in MS molecular images. Furthermore, DetectTLC can also spatially match m/z values for spots acquired during alternating high and low collision-energy scans, pairing product ions with precursors to enhance structural identification. As an example, DetectTLC is applied to the identification and structural confirmation of unknown, yet significant, products of abiotic pyrazinone and aminopyrazine nucleoside analog synthesis.

  5. Automated screening of reversed-phase stationary phases for small-molecule separations using liquid chromatography with mass spectrometry.

    Science.gov (United States)

    Appulage, Dananjaya K; Wang, Evelyn H; Carroll, Frances; Schug, Kevin A

    2016-05-01

    There are various reversed-phase stationary phases that offer significant differences in selectivity and retention. To investigate different reversed-phase stationary phases (aqueous stable C18 , biphenyl, pentafluorophenyl propyl, and polar-embedded alkyl) in an automated fashion, commercial software and associated hardware for mobile phase and column selection were used in conjunction with liquid chromatography and a triple quadrupole mass spectrometer detector. A model analyte mixture was prepared using a combination of standards from varying classes of analytes (including drugs, drugs of abuse, amino acids, nicotine, and nicotine-like compounds). Chromatographic results revealed diverse variations in selectivity and peak shape. Differences in the elution order of analytes on the polar-embedded alkyl phase for several analytes showed distinct selectivity differences compared to the aqueous C18 phase. The electron-rich pentafluorophenyl propyl phase showed unique selectivity toward protonated amines. The biphenyl phase provided further changes in selectivity relative to C18 with a methanolic phase, but it behaved very similarly to a C18 when an acetonitrile-based mobile phase was evaluated. This study shows the value of rapid column screening as an alternative to excessive mobile phase variation to obtain suitable chromatographic settings for analyte separation. PMID:26959840

  6. ETISEQ – an algorithm for automated elution time ion sequencing of concurrently fragmented peptides for mass spectrometry-based proteomics

    Directory of Open Access Journals (Sweden)

    Downard Kevin M

    2009-08-01

    Full Text Available Abstract Background Concurrent peptide fragmentation (i.e. shotgun CID, parallel CID or MSE has emerged as an alternative to data-dependent acquisition in generating peptide fragmentation data in LC-MS/MS proteomics experiments. Concurrent peptide fragmentation data acquisition has been shown to be advantageous over data-dependent acquisition by providing greater detection dynamic range and providing more accurate quantitative information. Nevertheless, concurrent peptide fragmentation data acquisition remains to be widely adopted due to the lack of published algorithms designed specifically to process or interpret such data acquired on any mass spectrometer. Results An algorithm called Elution Time Ion Sequencing (ETISEQ, has been developed to enable automated conversion of concurrent peptide fragmentation data acquisition data to LC-MS/MS data. ETISEQ generates MS/MS-like spectra based on the correlation of precursor and product ion elution profiles. The performance of ETISEQ is demonstrated using concurrent peptide fragmentation data from tryptic digests of standard proteins and whole influenza virus. It is shown that the number of unique peptides identified from the digests is broadly comparable between ETISEQ processed concurrent peptide fragmentation data and the data-dependent acquired LC-MS/MS data. Conclusion The ETISEQ algorithm has been designed for easy integration with existing MS/MS analysis platforms. It is anticipated that it will popularize concurrent peptide fragmentation data acquisition in proteomics laboratories.

  7. Concurrent automated sequencing of the glycan and peptide portions of O-linked glycopeptide anions by ultraviolet photodissociation mass spectrometry.

    Science.gov (United States)

    Madsen, James A; Ko, Byoung Joon; Xu, Hua; Iwashkiw, Jeremy A; Robotham, Scott A; Shaw, Jared B; Feldman, Mario F; Brodbelt, Jennifer S

    2013-10-01

    O-Glycopeptides are often acidic owing to the frequent occurrence of acidic saccharides in the glycan, rendering traditional proteomic workflows that rely on positive mode tandem mass spectrometry (MS/MS) less effective. In this report, we demonstrate the utility of negative mode ultraviolet photodissociation (UVPD) MS for the characterization of acidic O-linked glycopeptide anions. This method was evaluated for a series of singly and multiply deprotonated glycopeptides from the model glycoprotein kappa casein, resulting in production of both peptide and glycan product ions that afforded 100% sequence coverage of the peptide and glycan moieties from a single MS/MS event. The most abundant and frequent peptide sequence ions were a/x-type products which, importantly, were found to retain the labile glycan modifications. The glycan-specific ions mainly arose from glycosidic bond cleavages (B, Y, C, and Z ions) in addition to some less common cross-ring cleavages. On the basis of the UVPD fragmentation patterns, an automated database searching strategy (based on the MassMatrix algorithm) was designed that is specific for the analysis of glycopeptide anions by UVPD. This algorithm was used to identify glycopeptides from mixtures of glycosylated and nonglycosylated peptides, sequence both glycan and peptide moieties simultaneously, and pinpoint the correct site(s) of glycosylation. This methodology was applied to uncover novel site-specificity of the O-linked glycosylated OmpA/MotB from the "superbug" A. baumannii to help aid in the elucidation of the functional role that protein glycosylation plays in pathogenesis. PMID:24006841

  8. Automated on-line liquid-liquid extraction system for temporal mass spectrometric analysis of dynamic samples.

    Science.gov (United States)

    Hsieh, Kai-Ta; Liu, Pei-Han; Urban, Pawel L

    2015-09-24

    Most real samples cannot directly be infused to mass spectrometers because they could contaminate delicate parts of ion source and guides, or cause ion suppression. Conventional sample preparation procedures limit temporal resolution of analysis. We have developed an automated liquid-liquid extraction system that enables unsupervised repetitive treatment of dynamic samples and instantaneous analysis by mass spectrometry (MS). It incorporates inexpensive open-source microcontroller boards (Arduino and Netduino) to guide the extraction and analysis process. Duration of every extraction cycle is 17 min. The system enables monitoring of dynamic processes over many hours. The extracts are automatically transferred to the ion source incorporating a Venturi pump. Operation of the device has been characterized (repeatability, RSD = 15%, n = 20; concentration range for ibuprofen, 0.053-2.000 mM; LOD for ibuprofen, ∼0.005 mM; including extraction and detection). To exemplify its usefulness in real-world applications, we implemented this device in chemical profiling of pharmaceutical formulation dissolution process. Temporal dissolution profiles of commercial ibuprofen and acetaminophen tablets were recorded during 10 h. The extraction-MS datasets were fitted with exponential functions to characterize the rates of release of the main and auxiliary ingredients (e.g. ibuprofen, k = 0.43 ± 0.01 h(-1)). The electronic control unit of this system interacts with the operator via touch screen, internet, voice, and short text messages sent to the mobile phone, which is helpful when launching long-term (e.g. overnight) measurements. Due to these interactive features, the platform brings the concept of the Internet-of-Things (IoT) to the chemistry laboratory environment. PMID:26423626

  9. Effective action and electromagnetic response of topological superconductors and Majorana-mass Weyl fermions

    Science.gov (United States)

    Stone, Michael; Lopes, Pedro L. e. S.

    2016-05-01

    Motivated by an apparent paradox in [X.-L. Qi, E. Witten, and S.-C. Zhang, Phys. Rev. B 87, 134519 (2013), 10.1103/PhysRevB.87.134519], we use the method of gauged Wess-Zumino-Witten functionals to construct an effective action for a Weyl fermion with a Majorana mass that arises from coupling to a charged condensate. We obtain expressions for the current induced by an external gauge field and observe that the topological part of the current is only one-third of that that might have been expected from the gauge anomaly. The anomaly is not changed by the induced mass gap, however. The topological current is supplemented by a conventional supercurrent that provides the remaining two-thirds of the anomaly once the equation of motion for the Goldstone mode is satisfied. We apply our formula for the current to resolve the apparent paradox and also to the chiral magnetic effect (CME), where it predicts a reduction of the CME current to one-third of its value for a free Weyl gas in thermal equilibrium. We attribute this reduction to a partial cancellation of the CME by a chiral vortical effect current arising from the persistent rotation of the fluid induced by the external magnetic field.

  10. Actions taken on better earthquake resistance, level improvement and automation in the power distribution equipment; Haiden setsubi no taishin, kodoka, jidoka eno torikumi

    Energy Technology Data Exchange (ETDEWEB)

    Hara, N. [Agency of Natural Resources and Energy, Tokyo (Japan)

    1995-07-01

    This paper summarizes measures on earthquake resistance, level improvement and automation in power distribution equipment. Earthquake resistance in supports, transformers, wires, incoming lines, and underground wire ways has been handled in Japan according to the `technical criteria for electric facilities`. This is, however, compelled of reviewing with the great earthquake in 1995 as a turning point. Points of discussions include the review on earthquake resistance (considering earthquakes that occur directly below urban areas), improvement in urban power distribution network (network expansion and embedding the transmission lines), and emergency actions upon occurrence of disasters. The contents of the level improvement and automation may be summarized as follows: monitoring and control of distribution line devices including switch operation, automatic collection of power distribution management information intended of improving efficiency in the business operation, concentrated load control to achieve leveling of power loads, automatic meter-reading on power meters, and establishment of automatic and general power distribution system aiming at making common the use of devices, transmission paths, and signaling systems. The needs on information transmission utilizing power distribution equipment include stable power supply, diverse user services, and assurance of energy security. 2 figs.

  11. Automation of a flow injection system for the determination of dissolved silver at picomolar concentrations in seawater with inductively coupled plasma mass spectrometry

    OpenAIRE

    Achterberg, Eric P.; Truscott, Jason B.; Barriada, Jose L.

    2003-01-01

    An automated flow injection system for the determination of dissolved silver at ultratrace concentrations in seawater, and controlled under LabVIEW™, is described. The flow injection system allows online processing of seawater samples before their analysis using a magnetic sector inductively coupled plasma mass spectrometry (MS-ICP-MS) instrument. Samples were analysed with a minimum amount of manipulation, thereby reducing the risk of contamination. In addition, the flow injection approach w...

  12. Full mass range analysis of the QED effective action for an O(2)xO(3) symmetric field

    CERN Document Server

    Ahmadiniaz, Naser; Raya, Alfredo; Schubert, Christian

    2013-01-01

    An interesting class of background field configurations in quantum electrodynamics (QED) are the O(2)xO(3) symmetric fields, originally introduced by S.L. Adler in 1972. Those backgrounds have some instanton-like properties and yield a one-loop effective action that is highly nontrivial, but amenable to numerical calculation. Here, we use the recently developed "partial-wave-cutoff method" for a full mass range numerical analysis of the effective action for the "standard" O(2)xO(3) symmetric field, modified by a radial suppression factor. At large mass, we are able to match the asymptotics of the physically renormalized effective action against the leading two mass levels of the inverse mass expansion. For small masses, with a suitable choice of the renormalization scheme we obtain stable numerical results even in the massless limit. We analyze the N - point functions in this background and show that, even in the absence of the radial suppression factor, the two-point contribution to the effective action is t...

  13. New technologies in proteomics: automated excision and digestion of fluorescently stained protein gel spots and identification of proteins by MALDI-QTOF mass spectrometry

    International Nuclear Information System (INIS)

    This presentation is an overview of the techniques and systems we use for large-scale proteomics in plants. This includes a variety of 2D gel electrophoresis methods, and automation of spot-cutting, in-gel digestion, nanoLC-MS/MS tandem mass spectrometry and SEQUEST based protein identification. Examples and applications of several new technologies will also be shown, including the use of a robotic spot cutter in conjunction with high sensitivity fluorescent protein staining and the identification of proteins by redundant peptide identification in a high-throughput fashion using a hybrid MALDI-Qtof mass spectrometer

  14. Nonequilibrium Thermodynamic Formalism of Nonlinear Chemical Reaction Systems with Waage-Guldberg's Law of Mass Action

    CERN Document Server

    Ge, Hao

    2016-01-01

    Macroscopic entropy production $\\sigma^{(tot)}$ in the general nonlinear isothermal chemical reaction system with mass action kinetics is decomposed into a free energy dissipation and a house-keeping heat: $\\sigma^{(tot)}=\\sigma^{(fd)}+\\sigma^{(hk)}$; $\\sigma^{(fd)}=-\\rd A/\\rd t$, where $A$ is a generalized free enegy function. This yields a novel nonequilibrium free energy balance equation $\\rd A/\\rd t=-\\sigma^{(tot)}+\\sigma^{(hk)}$, which is on a par with celebrated entropy balance equation $\\rd S/\\rd t=\\sigma^{(tot)}+\\eta^{(ex)}$ where $\\eta^{(ex)}$ is the rate of entropy exchange with the environment.For kinetic system with complex balance,$\\sigma^{(fd)},\\sigma^{(hk)}\\ge 0$: $\\sigma^{(fd)}$ characterizes the irreversibility of a transient relaxation kinetics; while $\\sigma^{(hk)}$ is positive even in a steady state, representing irreversibility in open,driven chemical systems with a chemostat.For kinetic system withoutcomplex balance, negative $\\sigma^{(fd)}$ is a necessary condition for multistability, w...

  15. Kinetics of rouleau formation. I. A mass action approach with geometric features

    Energy Technology Data Exchange (ETDEWEB)

    Samsel, R.W. (Brown Univ., Providence, RI); Perelson, A.S.

    1982-02-01

    In the presence of certain macromolecules, such as fibrinogen, immunoglobulin, dextran, and polylysine, erythrocytes tend to aggregate and form cylindrical clusters called rouleaux in which cells resemble coins in a stack. The aggregates may remain cylindrical or they may branch, forming tree, and networklike structures. Using the law of mass action and notions from polymer chemistry, researchers derive expressions describing the kinetics of the early phase of aggregation. These models generalize work initiated by Ponder in 1927 who used the Smoluchowski equation to predict the concentration of rouleaux of different sizes. There are two novel features to this generalization. First, researchers allow erythrocytes that collide near the end of a stack of cells to move to the end of the cylinder and elongate it. Second, researchers incorporate geometric information into our models and describe the kinetics of branched rouleau formation. From these models one can predict the concentration of rouleaux with n cells and b branches, the mean number of cells per rouleau, the mean number of branches per rouleau, and the average length of a branch. Comparisons are made with the available experimental data.

  16. Determination of amlodipine in human plasma using automated online solid-phase extraction HPLC-tandem mass spectrometry: application to a bioequivalence study of Chinese volunteers.

    Science.gov (United States)

    Shentu, Jianzhong; Fu, Lizhi; Zhou, Huili; Hu, Xing Jiang; Liu, Jian; Chen, Junchun; Wu, Guolan

    2012-11-01

    An automated method (XLC-MS/MS) that uses online solid-phase extraction coupled with HPLC-tandem mass spectrometry was reported here for the first time to quantify amlodipine in human plasma. Automated pre-purification of plasma was performed using 10 mm × 2 mm HySphere C8 EC-SE online solid-phase extraction cartridges. After being eluted from the cartridge, the analyte and the internal standard were separated by HPLC and detected by tandem mass spectrometry. Mass spectrometric detection was achieved in the multiple reaction monitoring mode using a quadrupole tandem mass spectrometer in the positive electrospray ionization mode. The XLC-MS/MS method was validated and yielded excellent specificity. The calibration curve ranged from 0.10 to 10.22 ng/mL, and both the intra- and inter-day precision and accuracy values were within 8%. This method proved to be less laborious and was faster per analysis (high-throughput) than offline sample preparation methods. This method has been successfully applied in clinical pharmacokinetic and bioequivalence analyses. PMID:22770846

  17. Automation of dimethylation after guanidination labeling chemistry and its compatibility with common buffers and surfactants for mass spectrometry-based shotgun quantitative proteome analysis

    International Nuclear Information System (INIS)

    Graphical abstract: -- Highlights: •Dimethylation after guanidination (2MEGA) uses inexpensive reagents for isotopic labeling of peptides. •2MEGA can be optimized and automated for labeling peptides with high efficiency. •2MEGA is compatible with several commonly used cell lysis and protein solubilization reagents. •The automated 2MEGA labeling method can be used to handle a variety of protein samples for relative proteome quantification. -- Abstract: Isotope labeling liquid chromatography–mass spectrometry (LC–MS) is a major analytical platform for quantitative proteome analysis. Incorporation of isotopes used to distinguish samples plays a critical role in the success of this strategy. In this work, we optimized and automated a chemical derivatization protocol (dimethylation after guanidination, 2MEGA) to increase the labeling reproducibility and reduce human intervention. We also evaluated the reagent compatibility of this protocol to handle biological samples in different types of buffers and surfactants. A commercially available liquid handler was used for reagent dispensation to minimize analyst intervention and at least twenty protein digest samples could be prepared in a single run. Different front-end sample preparation methods for protein solubilization (SDS, urea, Rapigest™, and ProteaseMAX™) and two commercially available cell lysis buffers were evaluated for compatibility with the automated protocol. It was found that better than 94% desired labeling could be obtained in all conditions studied except urea, where the rate was reduced to about 92% due to carbamylation on the peptide amines. This work illustrates the automated 2MEGA labeling process can be used to handle a wide range of protein samples containing various reagents that are often encountered in protein sample preparation for quantitative proteome analysis

  18. Automation of dimethylation after guanidination labeling chemistry and its compatibility with common buffers and surfactants for mass spectrometry-based shotgun quantitative proteome analysis

    Energy Technology Data Exchange (ETDEWEB)

    Lo, Andy; Tang, Yanan; Chen, Lu; Li, Liang, E-mail: Liang.Li@ualberta.ca

    2013-07-25

    Graphical abstract: -- Highlights: •Dimethylation after guanidination (2MEGA) uses inexpensive reagents for isotopic labeling of peptides. •2MEGA can be optimized and automated for labeling peptides with high efficiency. •2MEGA is compatible with several commonly used cell lysis and protein solubilization reagents. •The automated 2MEGA labeling method can be used to handle a variety of protein samples for relative proteome quantification. -- Abstract: Isotope labeling liquid chromatography–mass spectrometry (LC–MS) is a major analytical platform for quantitative proteome analysis. Incorporation of isotopes used to distinguish samples plays a critical role in the success of this strategy. In this work, we optimized and automated a chemical derivatization protocol (dimethylation after guanidination, 2MEGA) to increase the labeling reproducibility and reduce human intervention. We also evaluated the reagent compatibility of this protocol to handle biological samples in different types of buffers and surfactants. A commercially available liquid handler was used for reagent dispensation to minimize analyst intervention and at least twenty protein digest samples could be prepared in a single run. Different front-end sample preparation methods for protein solubilization (SDS, urea, Rapigest™, and ProteaseMAX™) and two commercially available cell lysis buffers were evaluated for compatibility with the automated protocol. It was found that better than 94% desired labeling could be obtained in all conditions studied except urea, where the rate was reduced to about 92% due to carbamylation on the peptide amines. This work illustrates the automated 2MEGA labeling process can be used to handle a wide range of protein samples containing various reagents that are often encountered in protein sample preparation for quantitative proteome analysis.

  19. Development of an automated mass spectrometry system for the quantitative analysis of liver microsomal incubation samples: a tool for rapid screening of new compounds for metabolic stability.

    Science.gov (United States)

    Korfmacher, W A; Palmer, C A; Nardo, C; Dunn-Meynell, K; Grotz, D; Cox, K; Lin, C C; Elicone, C; Liu, C; Duchoslav, E

    1999-01-01

    There is a continuing need for increased throughput in the evaluation of new drug entities in terms of their pharmacokinetic parameters. One useful parameter that can be measured in vitro using liver microsomal preparations is metabolic stability. In this report, we describe an automated system that can be used for unattended quantitative analysis of liver microsomal samples for a series of compounds. This system is based on the Sciex API 150 (single quadrupole) liquid chromatography/mass spectrometry system and utilizes 96-well plate autosampler technology as well as a custom-designed AppleScript which executes the on-line data processing and report generation. It has the capability of analyzing at least 75 compounds per week or 300 compounds per month in an automated fashion. PMID:10353225

  20. Conditions for critical effects in the mass action kinetics equations for water radiolysis

    Energy Technology Data Exchange (ETDEWEB)

    Wittman, Richard S.; Buck, Edgar C.; Mausolf, Edward J.; McNamara, Bruce K.; Smith, Frances N.; Soderquist, Chuck Z.

    2014-12-26

    We report on a subtle global feature of the mass action kinetics equations for water radiolysis that results in predictions of a critical behavior in H2O2 and associated radical concentrations. While radiolysis kinetics has been studied extensively in the past, it is only in recent years that high speed computing has allowed the rapid exploration of the solution over widely varying dose and compositional conditions. We explore the radiolytic production of H2O2 under various externally fixed conditions of molecular H2 and O2 that have been regarded as problematic in the literature – specifically, “jumps” in predicted concentrations, and inconsistencies between predictions and experiments have been reported for alpha radiolysis. We computationally map-out a critical concentration behavior for alpha radiolysis kinetics using a comprehensive set of reactions. We then show that all features of interest are accurately reproduced with 15 reactions. An analytical solution for steady-state concentrations of the 15 reactions reveals regions in [H2] and [O2] where the H2O2 concentration is not unique – both stable and unstable concentrations exist. The boundary of this region can be characterized analytically as a function of G-values and rate constants independent of dose rate. Physically, the boundary can be understood as separating a region where a steady-state H2O2 concentration exists, from one where it does not exist without a direct decomposition reaction. We show that this behavior is consistent with reported alpha radiolysis data and that no such behavior should occur for gamma radiolysis. We suggest experiments that could verify or discredit a critical concentration behavior for alpha radiolysis and could place more restrictive ranges on G-values from derived relationships between them.

  1. Conditions for critical effects in the mass action kinetics equations for water radiolysis

    Energy Technology Data Exchange (ETDEWEB)

    Wittman, Richard S.; Buck, Edgar C.; Mausolf, Edward J.; McNamara, Bruce K.; Smith, Frances N.; Soderquist, Chuck Z.

    2014-11-25

    We report on a subtle global feature of the mass action kinetics equations for water radiolysis that results in predictions of a critical behavior in H2O2 and associated radical concentrations. While radiolysis kinetics has been studied extensively in the past, it is only in recent years that high speed computing has allowed the rapid exploration of the solution over widely varying dose and compositional conditions. We explore the radiolytic production of H2O2 under various externally fixed conditions of molecular H2 and O2 that have been regarded as problematic in the literature – specifically, “jumps” in predicted concentrations, and inconsistencies between predictions and experiments have been reported for alpha radiolysis. We computationally map-out a critical concentration behavior for alpha radiolysis kinetics using a comprehensive set of reactions. We then show that all features of interest are accurately reproduced with 15 reactions. An analytical solution for steady-state concentrations of the 15 reactions reveals regions in [H2] and [O2] where the H2O2 concentration is not unique – both stable and unstable concentrations exist. The boundary of this region can be characterized analytically as a function of G-values and rate constants independent of dose rate. Physically, the boundary can be understood as separating a region where a steady-state H2O2 concentration exists, from one where it does not exist without a direct decomposition reaction. We show that this behavior is consistent with reported alpha radiolysis data and that no such behavior should occur for gamma radiolysis. We suggest experiments that could verify or discredit a critical concentration behavior for alpha radiolysis and could place more restrictive ranges on G-values from derived relationships between them.

  2. Mass-action equilibrium and non-specific interactions in protein binding networks

    Science.gov (United States)

    Maslov, Sergei

    2009-03-01

    Large-scale protein binding networks serve as a paradigm of complex properties of living cells. These networks are naturally weighted with edges characterized by binding strength and protein-nodes -- by their concentrations. However, the state-of-the-art high-throughput experimental techniques generate just a binary (yes or no) information about individual interactions. As a result, most of the previous research concentrated just on topology of these networks. In a series of recent publications [1-4] my collaborators and I went beyond purely topological studies and calculated the mass-action equilibrium of a genome-wide binding network using experimentally determined protein concentrations, localizations, and reliable binding interactions in baker's yeast. We then studied how this equilibrium responds to large perturbations [1-2] and noise [3] in concentrations of proteins. We demonstrated that the change in the equilibrium concentration of a protein exponentially decays (and sign-alternates) with its network distance away from the perturbed node. This explains why, despite a globally connected topology, individual functional modules in such networks are able to operate fairly independently. In a separate study [4] we quantified the interplay between specific and non-specific binding interactions under crowded conditions inside living cells. We show how the need to limit the waste of resources constrains the number of types and concentrations of proteins that are present at the same time and at the same place in yeast cells. [1] S Maslov, I. Ispolatov, PNAS 104:13655 (2007). [2] S. Maslov, K. Sneppen, I. Ispolatov, New J. of Phys. 9: 273 (2007). [3] K-K. Yan, D. Walker, S. Maslov, PRL accepted (2008). [4] J. Zhang, S. Maslov, and E. I. Shakhnovich, Mol Syst Biol 4, 210 (2008).

  3. Cato Guldberg and Peter Waage, the history of the Law of Mass Action, and its relevance to clinical pharmacology.

    Science.gov (United States)

    Ferner, Robin E; Aronson, Jeffrey K

    2016-01-01

    We have traced the historical link between the Law of Mass Action and clinical pharmacology. The Law evolved from the work of the French chemist Claude Louis Berthollet, was first formulated by Cato Guldberg and Peter Waage in 1864 and later clarified by the Dutch chemist Jacobus van 't Hoff in 1877. It has profoundly influenced our qualitative and quantitative understanding of a number of physiological and pharmacological phenomena. According to the Law of Mass Action, the velocity of a chemical reaction depends on the concentrations of the reactants. At equilibrium the concentrations of the chemicals involved bear a constant relation to each other, described by the equilibrium constant, K. The Law of Mass Action is relevant to various physiological and pharmacological concepts, including concentration-effect curves, dose-response curves, and ligand-receptor binding curves, all of which are important in describing the pharmacological actions of medications, the Langmuir adsorption isotherm, which describes the binding of medications to proteins, activation curves for transmembrane ion transport, enzyme inhibition and the Henderson-Hasselbalch equation, which describes the relation between pH, as a measure of acidity and the concentrations of the contributory acids and bases. Guldberg and Waage recognized the importance of dynamic equilibrium, while others failed to do so. Their ideas, over 150 years old, are embedded in and still relevant to clinical pharmacology. Here we explain the ideas and in a subsequent paper show how they are relevant to understanding adverse drug reactions. PMID:26174880

  4. Calculating models of mass action concentrations for NaBr(aq), LiNO3(aq),HNO3(aq), and KF(aq) binary solutions

    Institute of Scientific and Technical Information of China (English)

    Hanjie Guo; Weijie Zhao; Xuemin Yang

    2007-01-01

    The calculating models of mass action concentrations for electrolyte aqueous solutions NaBr-H2O, LiNO3-H2O, HNO3-H2O,and KF-H2O have been developed at 298.15 K and their molalities ranging from 0.1 mol/kg to saturation according to the ion and molecule coexistence theory as well as mass action law. The calculated mass action concentration is based on pure species as the standard state and the mole fraction as the concentration unit, and the reported activities are usually based on infinite dilution as the standard state and molality as the concentration unit. Hence, the calculated mass action concentration must be transformed to the same standard state and concentration unit. The transformation coefficients between calculated mass action concentrations and reported activities of the same component fluctuate in a very narrow range. Thus, the transformed mass action concentrations not only agree well with reported activities, but also strictly obey mass action law. The calculated results show that the new developed models can embody the intrinsic structure of investigated four electrolyte aqueous solutions. The results also indicate that mass action law has its widespread applicability to electrolyte binary aqueous solutions.

  5. Oak Ridge K-25 Site Technology Logic Diagram. Volume 3, Technology evaluation data sheets; Part B, Remedial action, robotics/automation, waste management

    Energy Technology Data Exchange (ETDEWEB)

    Fellows, R.L. [ed.

    1993-02-26

    The Oak Ridge K-25 Technology Logic Diagram (TLD), a decision support tool for the K-25 Site, was developed to provide a planning document that relates environmental restoration (ER) and waste management (WN) problems at the Oak Ridge K-25 Site. The TLD technique identifies the research necessary to develop these technologies to a state that allows for technology transfer and application to waste management, remediation, decontamination, and decommissioning activities. The TLD consists of four separate volumes-Vol. 1, Vol. 2, Vol. 3A, and Vol. 3B. Volume 1 provides introductory and overview information about the TLD. Volume 2 contains logic diagrams. Volume 3 has been divided into two separate volumes to facilitate handling and use. This volume 3 B provides the Technology Evaluation Data Sheets (TEDS) for ER/WM activities (Remedial Action Robotics and Automation, Waste Management) that are referenced by a TEDS code number in Vol. 2 of the TLD. Each of these sheets represents a single logic trace across the TLD. These sheets contain more detail than each technology in Vol. 2. The TEDS are arranged alphanumerically by the TEDS code number in the upper right corner of each data sheet. Volume 3 can be used in two ways: (1) technologies that are identified from Vol. 2 can be referenced directly in Vol. 3 by using the TEDS codes, and (2) technologies and general technology areas (alternatives) can be located in the index in the front of this volume.

  6. Coupling Immobilized Alkaline Phosphatase-based Automated Diagonal Capillary Electrophoresis to Tandem Mass Spectrometry for Phosphopeptide Analysis

    OpenAIRE

    Mou, Si; Sun, Liangliang; Wojcik, Roza; Dovichi, Norman J.

    2013-01-01

    Automated diagonal capillary electrophoresis is a two-dimensional separation method that incorporates an immobilized enzyme reactor at the distal end of the first capillary and employs identical electrophoretic separation modes in both dimensions. Components undergo a preliminary separation in the first capillary. Fractions are parked in the reactor where some components undergo transformation. The fractions are then periodically transferred to the second capillary and replaced by the next co...

  7. Mass Spectrometry-Based Monitoring of Millisecond Protein-Ligand Binding Dynamics Using an Automated Microfluidic Platform

    Energy Technology Data Exchange (ETDEWEB)

    Cong, Yongzheng; Katipamula, Shanta; Trader, Cameron D.; Orton, Daniel J.; Geng, Tao; Baker, Erin Shammel; Kelly, Ryan T.

    2016-03-24

    Characterizing protein-ligand binding dynamics is crucial for understanding protein function and developing new therapeutic agents. We have developed a novel microfluidic platform that features rapid mixing of protein and ligand solutions, variable incubation times, and on-chip electrospray ionization to perform label-free, solution-based monitoring of protein-ligand binding dynamics. This platform offers many advantages including automated processing, rapid mixing, and low sample consumption.

  8. Pseudoscalar decay constants, light-quark masses, and B_K from mixed-action lattice QCD

    CERN Document Server

    Laiho, Jack

    2011-01-01

    We present updated results for the leptonic decay constants f_pi and f_K, the light u, d, and s-quark masses, and the neutral kaon mixing parameter B_K from mixed-action lattice simulations with staggered sea quarks and domain-wall valence quarks. We use the publicly-available 2+1 flavor MILC asqtad-improved staggered gauge configurations with multiple light sea-quark masses and three lattice spacings, and compute the kaon mixing matrix element with several partially-quenched valence-quark masses. We then extrapolate to the physical light-quark masses and the continuum using partially-quenched chiral perturbation theory formulated for mixed-action lattice simulations. For B_K we match the lattice four-fermion operator to the continuum using the nonperturbative method of Rome-Southampton. Our new results benefit from two significant improvements over our published work: (1) we have added a third lattice spacing of a~0.06 fm to better control the continuum extrapolation, and (2) we have implemented a new lattic...

  9. APPLICABILITY OF THE MASS ACTION LAW IN COMBINATION WITH THE COEXISTENCE THEORY OF METALLIC MELTS INVOLVING COMPOUND TO BINARY METALLIC MELTS

    Institute of Scientific and Technical Information of China (English)

    J. Zhang

    2002-01-01

    Based on the atomicity and molecularity as well as the consistency of thermodynamicproperties and activities of metallic melts with their structures, the coexistence the-ory of metallic melts structure involving compound has been suggested. According tothis theory, the calculating models of mass action concentrations for different binarymetallic melts have been formulated. The calculated mass action concentrations agreewell with corresponding measured activities, which confirms that the suggested theorycan reflect the structural characteristics of metallic melts involving compound and thatthe mass action law is widely applicable to this kind of metallic melts.

  10. The antimicrobial action of low-molar-mass chitosan, chitosan derivates and chitooligosaccharides on bifidobacteria

    Czech Academy of Sciences Publication Activity Database

    Šimůnek, Jiří; Koppová, Ingrid; Lukáš, Filip; Tishchenko, Galina; Belzecki, G.

    2010-01-01

    Roč. 55, č. 4 (2010), s. 379-382. ISSN 0015-5632 R&D Projects: GA ČR(CZ) GA525/08/0803 Institutional research plan: CEZ:AV0Z50450515; CEZ:AV0Z40500505 Keywords : chitooligosaccharides * low-molar-mass chitosan Subject RIV: EE - Microbiology, Virology Impact factor: 0.977, year: 2010

  11. Joint Actions of the Republic of Tajikistan and the United States of America in the Field of Weapons of Mass Destruction Non-Proliferation

    International Nuclear Information System (INIS)

    The paper describes the legislative basis in the field of radiation safety and security and regulatory authority establishment in Tajikistan. Joint actions undertaken after the civil war in Tajikistan by regulatory authorities of the Governments of the United States of America and Tajikistan are presented. Actions performed and planned in order to prevent proliferation of weapons of mass destruction in Tajikistan are presented. (author)

  12. Differential fragmentation patterns of pectin oligogalacturonides observed by nanoelectrospray quadrupole ion-trap mass spectrometry using automated spectra interpretation

    DEFF Research Database (Denmark)

    Mutenda, Kudzai E; Matthiesen, Rune; Roepstorff, Peter

    2007-01-01

    Oligogalacturonides of different degrees of polymerization (DP) and methyl esterification (DE) were structurally analyzed by nanoESI quadrupole ion-trap mass spectrometry. The fragmentation patterns of the oligogalacturonides were compared using the program 'Virtual Expert Mass Spectrometrist...... with free carboxylic acid groups underwent higher water loss compared to fully methyl-esterified oligogalacturonides under the same fragmentation conditions. Cross-ring cleavage, in which fragmentation occurs across the ring system of the galacturonate residue and signified by unique mass losses, was...... importance in pectin chemistry, where mass spectrometry has become the technique of choice for structural determination. Although this study was not designed to explain the mechanisms of oligogalacturonide fragmentation, possible explanations for why non-methyl-esterified oligogalacturonides undergo more...

  13. Determination of Low Concentrations of Acetochlor in Water by Automated Solid-Phase Extraction and Gas Chromatography with Mass-Selective Detection

    Science.gov (United States)

    Lindley, C.E.; Stewart, J.T.; Sandstrom, M.W.

    1996-01-01

    A sensitive and reliable gas chromatographic/mass spectrometric (GC/MS) method for determining acetochlor in environmental water samples was developed. The method involves automated extraction of the herbicide from a filtered 1 L water sample through a C18 solid-phase extraction column, elution from the column with hexane-isopropyl alcohol (3 + 1), and concentration of the extract with nitrogen gas. The herbicide is quantitated by capillary/column GC/MS with selected-ion monitoring of 3 characteristic ions. The single-operator method detection limit for reagent water samples is 0.0015 ??g/L. Mean recoveries ranged from about 92 to 115% for 3 water matrixes fortified at 0.05 and 0.5 ??g/L. Average single-operator precision, over the course of 1 week, was better than 5%.

  14. Enantioselective determination of methylphenidate and ritalinic acid in whole blood from forensic cases using automated solid-phase extraction and liquid chromatography-tandem mass spectrometry

    DEFF Research Database (Denmark)

    Thomsen, Ragnar; B. Rasmussen, Henrik; Linnet, Kristian;

    2012-01-01

    A chiral liquid chromatography tandem mass spectrometry (LC–MS-MS) method was developed and validated for quantifying methylphenidate and its major metabolite ritalinic acid in blood from forensic cases. Blood samples were prepared in a fully automated system by protein precipitation followed by...... methylphenidate was not determined to be related to the cause of death, the femoral blood concentration of d-methylphenidate ranged from 5 to 58 ng/g, and from undetected to 48 ng/g for l-methylphenidate (median d/l-ratio 5.9). Ritalinic acid was present at concentrations 10–20 times higher with roughly equal...... amounts of the d- and l-forms. In blood from 10 living subjects that were not suspected of being intoxicated by methylphenidate, the concentration ranges and patterns were similar to those of the postmortem cases. Thus, methylphenidate does not appear to undergo significant postmortem redistribution....

  15. Single-core magnetic markers in rotating magnetic field based homogeneous bioassays and the law of mass action

    Energy Technology Data Exchange (ETDEWEB)

    Dieckhoff, Jan, E-mail: j.dieckhoff@tu-bs.de [Institut fuer Elektrische Messtechnik und Grundlagen der Elektrotechnik, TU Braunschweig, Braunschweig (Germany); Schrittwieser, Stefan; Schotter, Joerg [Molecular Diagnostics, AIT Austrian Institute of Technology, Vienna (Austria); Remmer, Hilke; Schilling, Meinhard; Ludwig, Frank [Institut fuer Elektrische Messtechnik und Grundlagen der Elektrotechnik, TU Braunschweig, Braunschweig (Germany)

    2015-04-15

    In this work, we report on the effect of the magnetic nanoparticle (MNP) concentration on the quantitative detection of proteins in solution with a rotating magnetic field (RMF) based homogeneous bioassay. Here, the phase lag between 30 nm iron oxide single-core particles and the RMF is analyzed with a fluxgate-based measurement system. As a test analyte anti-human IgG is applied which binds to the protein G functionalized MNP shell and causes a change of the phase lag. The measured phase lag changes for a fixed MNP and a varying analyte concentration are modeled with logistic functions. A change of the MNP concentration results in a nonlinear shift of the logistic function with the analyte concentration. This effect results from the law of mass action. Furthermore, the bioassay results are used to determine the association constant of the binding reaction. - Highlights: • A rotating magnetic field based homogeneous bioassay concept was presented. • Here, single-core iron oxide nanoparticles are applied as markers. • The impact of the particle concentration on the bioassay results is investigated. • The relation between particle concentration and bioassay sensitivity is nonlinear. • This finding can be reasonably explained by the law of mass action.

  16. Single-core magnetic markers in rotating magnetic field based homogeneous bioassays and the law of mass action

    International Nuclear Information System (INIS)

    In this work, we report on the effect of the magnetic nanoparticle (MNP) concentration on the quantitative detection of proteins in solution with a rotating magnetic field (RMF) based homogeneous bioassay. Here, the phase lag between 30 nm iron oxide single-core particles and the RMF is analyzed with a fluxgate-based measurement system. As a test analyte anti-human IgG is applied which binds to the protein G functionalized MNP shell and causes a change of the phase lag. The measured phase lag changes for a fixed MNP and a varying analyte concentration are modeled with logistic functions. A change of the MNP concentration results in a nonlinear shift of the logistic function with the analyte concentration. This effect results from the law of mass action. Furthermore, the bioassay results are used to determine the association constant of the binding reaction. - Highlights: • A rotating magnetic field based homogeneous bioassay concept was presented. • Here, single-core iron oxide nanoparticles are applied as markers. • The impact of the particle concentration on the bioassay results is investigated. • The relation between particle concentration and bioassay sensitivity is nonlinear. • This finding can be reasonably explained by the law of mass action

  17. Use of mass and toxicity balances in risk-based corrective action decisions at contaminated sites

    International Nuclear Information System (INIS)

    The contaminated groundwater at a sour gas plant facility was studied to identify the chemicals of environmental concern. Simple mass balance principles were used to determine the proportion of organic carbon, organic nitrogen and MicrotoxR toxicity that can be attributed to two process chemicals that have contaminated several sour gas plants in western Canada. The two process chemicals are sulfolane and diisopropanolamine (DIPA). The organic carbon balance was calculated by determining the molar contribution of sulfolane and DIPA relative to the mass of carboxylic acid-corrected dissolved organic carbon. Organic carbon balances ranged from 44 to 96 per cent. The organic nitrogen balance was calculated by determining the molar contribution of DIPA relative to the mass of ammonium ion-corrected dissolved Kjeldahl nitrogen. The nitrogen balances were highly variable between 8 to 48 per cent for samples with organic nitrogen concentrations between 10 and 32 mg/L. The MicrotoxR toxicity balance was calculated by determining the proportions of toxicity that could be accounted for by pure phase sulfolane and DIPA. The MicrotoxR toxicity balance for samples that showed significant toxicity ranged from 71 to 122 per cent

  18. Effects of mass media action on the Axelrod model with social influence

    Science.gov (United States)

    Rodríguez, Arezky H.; Moreno, Y.

    2010-07-01

    The use of dyadic interaction between agents, in combination with homophily (the principle that “likes attract”) in the Axelrod model for the study of cultural dissemination, has two important problems: the prediction of monoculture in large societies and an extremely narrow window of noise levels in which diversity with local convergence is obtained. Recently, the inclusion of social influence has proven to overcome them [A. Flache and M. W. Macy, e-print arXiv:0808.2710]. Here, we extend the Axelrod model with social influence interaction for the study of mass media effects through the inclusion of a superagent which acts over the whole system and has non-null overlap with each agent of the society. The dependence with different parameters as the initial social diversity, size effects, mass media strength, and noise is outlined. Our results might be relevant in several socioeconomic contexts and for the study of the emergence of collective behavior in complex social systems.

  19. Effects of Mass Media action on the Axelrod Model with Social Influence

    CERN Document Server

    Rodríguez, Arezky H

    2010-01-01

    The use of {\\it dyadic interaction} between agents, in combination with {\\it homophily} (the principle that ``likes attract'') in the Axelrod model for the study of cultural dissemination has two important problems: the prediction of monoculture in large societies and an extremely narrow window of noise levels in which diversity with local convergence is obtained. Recently, the inclusion of {\\it social influence} has proven to overcome them (A. Flache and M. W. Macey, arXiv:0808.2710). Here we extend the Axelrod model with social influence interaction for the study of Mass Media effects through the inclusion of a super-agent which acts over the whole system and has nonnull overlap with each agent of the society. The dependence with different parameters as the initial social diversity, size effects, Mass Media strength and noise is outlined. Our results might be relevant in several socio-economic contexts and for the study of the emergence of collective behavior in complex social systems.

  20. Coupling of fully automated chip-based electrospray ionization to high-capacity ion trap mass spectrometer for ganglioside analysis.

    Science.gov (United States)

    Almeida, Reinaldo; Mosoarca, Cristina; Chirita, Marius; Udrescu, Valentina; Dinca, Nicolae; Vukelić, Zeljka; Allen, Mark; Zamfir, Alina D

    2008-07-01

    NanoMate robot was coupled to a high-capacity ion trap (HCT) mass spectrometer to create a system merging automatic chip-based electrospray ionization (ESI) infusion, ultrafast ion detection, and multistage sequencing at superior sensitivity. The interface between the NanoMate and HCT mass spectrometer consists of an in-laboratory constructed mounting device that allows adjustment of the robot position with respect to the mass spectrometer inlet. The coupling was optimized for ganglioside (GG) high-throughput analysis in the negative ion mode and was implemented in clinical glycolipidomics for identification and structural characterization of anencephaly-associated species. By NanoMate HCT mass spectrometry (MS), data corroborating significant differences in GG expression in anencephalic versus age-matched normal brain tissue were collected. The feasibility of chip-based nanoESI HCT multistage collision-induced dissociation (CID MS(n)) for polysialylated GG fragmentation and isomer discrimination was tested on a GT1 (d18:1/18:0) anencephaly-associated structure. MS(2)-MS(4) obtained by accumulating scans at variable fragmentation amplitudes gave rise to the first fragmentation patterns from which the presence of GT1b structural isomer could be determined unequivocally without the need for supplementary investigation by any other analytical or biochemical methods. PMID:18406832

  1. metAlignID: A high-throughout sofware tool set for automated detection of trace level contaminants in comprehensive LECO two-dimensional gas chromatography time-of-flight mass spectrometry data

    NARCIS (Netherlands)

    Lommen, A.; Kamp, van der H.J.; Kools, H.J.; Lee, van der M.K.; Weg, van der G.

    2012-01-01

    A new alternative data processing tool set, metAlignID, is developed for automated pre-processing and library-based identification and concentration estimation of target compounds after analysis by comprehensive two-dimensional gas chromatography with mass spectrometric detection. The tool set has b

  2. Automated mini-column solid-phase extraction cleanup for high-throughput analysis of chemical contaminants in foods by low-pressure gas chromatography – tandem mass spectrometry

    Science.gov (United States)

    This study demonstrated the application of an automated high-throughput mini-cartridge solid-phase extraction (mini-SPE) cleanup for the rapid low-pressure gas chromatography – tandem mass spectrometry (LPGC-MS/MS) analysis of pesticides and environmental contaminants in QuEChERS extracts of foods. ...

  3. A METHOD FOR AUTOMATED ANALYSIS OF 10 ML WATER SAMPLES CONTAINING ACIDIC, BASIC, AND NEUTRAL SEMIVOLATILE COMPOUNDS LISTED IN USEPA METHOD 8270 BY SOLID PHASE EXTRACTION COUPLED IN-LINE TO LARGE VOLUME INJECTION GAS CHROMATOGRAPHY/MASS SPECTROMETRY

    Science.gov (United States)

    Data is presented showing the progress made towards the development of a new automated system combining solid phase extraction (SPE) with gas chromatography/mass spectrometry for the single run analysis of water samples containing a broad range of acid, base and neutral compounds...

  4. LipidMiner: A Software for Automated Identification and Quantification of Lipids from Multiple Liquid Chromatography-Mass Spectrometry Data Files

    Energy Technology Data Exchange (ETDEWEB)

    Meng, Da; Zhang, Qibin; Gao, Xiaoli; Wu, Si; Lin, Guang

    2014-04-30

    We have developed a tool for automated, high-throughput analysis of LC-MS/MS data files, which greatly simplifies LC-MS based lipidomics analysis. Our results showed that LipidMiner is accurate and comprehensive in identification and quantification of lipid molecular species. In addition, the workflow implemented in LipidMiner is not limited to identification and quantification of lipids. If a suitable metabolite library is implemented in the library matching module, LipidMiner could be reconfigured as a tool for general metabolomics data analysis. It is of note that LipidMiner currently is limited to singly charged ions, although it is adequate for the purpose of lipidomics since lipids are rarely multiply charged,[14] even for the polyphosphoinositides. LipidMiner also only processes file formats generated from mass spectrometers from Thermo, i.e. the .RAW format. In the future, we are planning to accommodate file formats generated by mass spectrometers from other predominant instrument vendors to make this tool more universal.

  5. ARTEMiS (Automated Robotic Terrestrial Exoplanet Microlensing Search) - A possible expert-system based cooperative effort to hunt for planets of Earth mass and below

    CERN Document Server

    Dominik, M; Allan, A; Rattenbury, N J; Tsapras, Y; Snodgrass, C; Bode, M F; Burgdorf, M J; Fraser, S N; Kerins, E; Mottram, C J; Steele, I A; Street, R A; Wheatley, P J; Wyrzykowski, L

    2008-01-01

    (abridged) The technique of gravitational microlensing is currently unique in its ability to provide a sample of terrestrial exoplanets around both Galactic disk and bulge stars, allowing to measure their abundance and determine their distribution with respect to mass and orbital separation. In order to achieve these goals in reasonable time, a well-coordinated effort involving a network of either 2m or 4 x 1m telescopes at each site is required. It could lead to the first detection of an Earth-mass planet outside the Solar system, and even planets less massive than Earth could be discovered. From April 2008, ARTEMiS (Automated Robotic Terrestrial Exoplanet Microlensing Search) is planned to provide a platform for a three-step strategy of survey, follow-up, and anomaly monitoring. As an expert system embedded in eSTAR (e-Science Telescopes for Astronomical Research), ARTEMiS will give advice on the optimal targets to be observed at any given time, and will also alert on deviations from ordinary microlensing l...

  6. Concurrent Automated Sequencing of the Glycan and Peptide Portions of O-Linked Glycopeptide Anions by Ultraviolet Photodissociation Mass Spectrometry

    OpenAIRE

    Madsen, James A.; Ko, Byoung Joon; Xu, Hua; Iwashkiw, Jeremy A; Robotham, Scott A.; Shaw, Jared B.; Feldman, Mario F.; Brodbelt, Jennifer S.

    2013-01-01

    O -glycopeptides are often acidic owing to the frequent occurrence of acidic saccharides in the glycan, rendering traditional proteomic workflows that rely on positive mode tandem mass spectrometry (MS/MS) less effective. In this report, we demonstrate the utility of negative mode ultraviolet photodissociation (UVPD) MS for the characterization of acidic O-linked glycopeptide anions. This method was evaluated for a series of singly- and multiply-deprotonated glycopeptides from the model glyco...

  7. Fully automated analysis of beta-lactams in bovine milk by online solid phase extraction-liquid chromatography-electrospray-tandem mass spectrometry.

    Science.gov (United States)

    Kantiani, Lina; Farré, Marinella; Sibum, Martin; Postigo, Cristina; López de Alda, Miren; Barceló, Damiá

    2009-06-01

    A fully automated method for the detection of beta-lactam antibiotics, including six penicillins (amoxicillin, ampicillin, cloxacillin, dicloxacillin, oxacillin, and penicillin G) and four cephalosporins (cefazolin, ceftiofur, cefoperazone, and cefalexin) in bovine milk samples has been developed. The outlined method is based on online solid-phase extraction-liquid chromatography/electrospray-tandem mass spectrometry (SPE-LC/ESI-MS-MS). Target compounds were concentrated from 500 microL of centrifuged milk samples using an online SPE procedure with C18 HD cartridges. Target analytes were eluted with a gradient mobile phase (water + 0.1% formic acid/methanol + 0.1% formic acid) at a flow rate of 0.7 mL/min. Chromatographic separation was achieved within 10 min using a C-12 reversed phase analytical column. For unequivocal identification and confirmation, two multiple reaction monitoring (MRM) transitions were acquired for each analyte in the positive electrospray ionization mode (ESI(+)). Method limits of detection (LODs) in milk were well below the maximum residue limits (MRLs) set by the European Union for all compounds. Limits of quantification in milk were between 0.09 ng/mL and 1.44 ng/mL. The developed method was validated according to EU's requirements, and accuracy results ranged from 80 to 116%. Finally, the method was applied to the analysis of twenty real samples previously screened by the inhibition of microbial growth test Eclipse 100. This new developed method offers high sensitivity and accuracy of results, minimum sample pre-treatment, and uses for the first time an automated online SPE offering a high throughput analysis. Because of all these characteristics, the proposed method is applicable and could be deemed necessary within the field of food control and safety. PMID:19402673

  8. Ab initio free energy of vacancy formation and mass-action kinetics in vis-active TiO2

    International Nuclear Information System (INIS)

    Recent reports have identified bulk defects such as oxygen vacancies as key players in visible-light photoactive TiO2. This would imply greater visible light absorption rates may be possible provided effective defect engineering can be achieved. To further this we have developed methods to simulate vacancy formation in bulk TiO2 using ab initio techniques. Initial results of these methods show an entropic reduction in the free energy of vacancy formation of 2.3 eV over a range of 266 K. The use of this result is illustrated by a 'toy' mass-action kinetics model which offers insight into vacancy concentration, rate constants, and enthalpy of reaction. (fast track communication)

  9. Home Automation

    OpenAIRE

    Ahmed, Zeeshan

    2010-01-01

    In this paper I briefly discuss the importance of home automation system. Going in to the details I briefly present a real time designed and implemented software and hardware oriented house automation research project, capable of automating house's electricity and providing a security system to detect the presence of unexpected behavior.

  10. Integration of biotechnology, robot technology and visualisation technology for development of methods for automated mass production of elite trees

    DEFF Research Database (Denmark)

    Find, Jens

    sterile in vitro conditions to non sterile (ex vitro/in vivo) conditions at the nursery. Fig. 1. Predicted labour cost by clonal propagation through somatic embryogenesis.   The labour costs are very low in the early steps of the production, whereas they increase dramatically during the later stages......Biotechnology has become an integrated part of plant breeding, and in recent years new methods have been developed for breeding and propagation of important plants in the agricultural-, ornamental- and forestry sector. One of the promising methods is somatic embryogenesis (SE), where plants are...... produced from single cells without sexual reproduction. SE has some particular advantages for the development of cost effective methods for clonal mass propagation of elite plants: It is a very effective and fast method for clonal propagation. The method is suitable for automatisation and robot technology...

  11. Mass data massage: an automated data processing system used for NHEXAS, Arizona. National Human Exposure Assessment Survey.

    Science.gov (United States)

    O'Rourke, M K; Fernandez, L M; Bittel, C N; Sherrill, J L; Blackwell, T S; Robbins, D R

    1999-01-01

    Data entry and management are critical components of all large survey projects; data quality objectives must be met and data must be quickly and readily accessible. We developed a comprehensive system for data entry and management utilizing scannable forms with bubble fields and handwriting recognition. This 'Mass Data Massage' (MDM) system had three components: (1) form creation and database definition; (2) programming of data dictionaries for documentation and preliminary logic and range checks; and (3) data entry, management and documentation using the 'Mass Data Cleaning Program' (MDCP). Scannable forms were written in Teleform, where the data field definition, variable names and ranges were defined as the form was created. Completed forms were returned from the field, subjected to final field quality control (QC) checks, and transferred to the data management section. They were batched and coded as necessary. Once a batch of data was scanned and visually verified, the operator called up the menu for the MDCP. The MDCP had 31 program modules with 500-1200 lines of code each. The operator could select and run the appropriate dictionary on each data batch 'correcting' apparent errors in responses. This process was iterative until the data batch passed all dictionary checks. Proposed 'changes' were forwarded to the data coordinator (DC) for acceptance or rejection. After all errors had been resolved, each data batch was subjected to a 10% quality assurance (QA) check. The original data batch and associated file of applied changes were archived. Time expenditure using the scanning approach varied with the number of questions and the types of responses (handwritten or bubble fields). One-page forms took 42-60% of the time needed for hand entry; forms longer than 10 pages took 35-38% of the time. Use of faster machines will further speed the process. The main advantage of the system was the reduction of systematic errors. Scanning alone reduced errors found on 995

  12. Calculation Model of Mass Action Concentration for Mg-Al, Sr-Al and Ba-Al Melts and Determination of Their Thermodynamic Parameters

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Based on the phase diagrams and the mass action law in combination with the coexistence theory of metallic melts structure, the calculation model of mass action concentration for Mg-Al, Sr-Al and Ba-Al was built, and their thermodynamic parameters were determined. The agreement between calculated and measured results shows that the model and the determined thermodynamic parameters can reflect the structural characteristics of relevant melts. However, the fact that the thermodynamic parameters from literature don′t give the value agree with the measured result may be due to unconformity of these parameters to real chemical reactions in metallic melts.

  13. Automated storage of gas chromatography-mass spectrometry data in a relational database to facilitate compound screening and identification.

    Science.gov (United States)

    Staeb, J A; Epema, O J; van Duijn, P; Steevens, J; Klap, V A; Freriks, I L

    2002-10-18

    This paper describes a database containing massspectra from gas chromatography-mass spectrometry(GC-MS) measurements as a tool for easy screening for multiple compounds. In this way additional compounds can be reported from the same run together with routine pesticide monitoring with little effort. The relevant analytical data from the GC-MS measurements are transferred automatically to a database. Search algorithms in the database, containing the US EPA and Dutch NEN GC-MS identification criteria as standard settings, are used to identify compounds in the data. Screening of samples analysed in our laboratory show the ubiquitous presence of--up until now in monitoring largely overlooked--compounds in surface waters in The Netherlands. Most frequently found compounds include TAED (complexing agent), 2-methyl quinoline (industrial solvent), atrazin and desethylatrazin (pesticide and degradation product), caffeine (human consumption), surfinol-104 (anti foaming agent), HHCB (Galaxolide) and AHTN (Tonalide; fragrances). The database can also be used to quickly search a large number of datafiles for rare contaminants. This way, some interesting compounds such as pentoxifilin (a pharmaceutical) and Irgarol 1051 (an antifouling compound) were found. PMID:12458939

  14. Multiresidue trace analysis of pharmaceuticals, their human metabolites and transformation products by fully automated on-line solid-phase extraction-liquid chromatography-tandem mass spectrometry.

    Science.gov (United States)

    García-Galán, María Jesús; Petrovic, Mira; Rodríguez-Mozaz, Sara; Barceló, Damià

    2016-09-01

    A novel, fully automated analytical methodology based on dual column liquid chromatography coupled to tandem mass spectrometry (LC-LC-MS(2)) has been developed and validated for the analysis of 12 pharmaceuticals and 20 metabolites and transformation products in different types of water (influent and effluent wastewaters and surface water). Two LC columns were used - one for pre-concentration of the sample and the second for separation and analysis - so that water samples were injected directly in the chromatographic system. Besides the many advantages of the methodology, such as minimization of the sample volume required and its manipulation, both compounds ionized in positive and negative mode could be analyzed simultaneously without compromising the sensitivity. A comparative study of different mobile phases, gradients and LC pre-concentration columns was carried out to obtain the best analytical performance. Limits of detection (MLODs) achieved were in the low ngL(-1) range for all the compounds. The method was successfully applied to study the presence of the target analytes in different wastewater and surface water samples collected near the city of Girona (Catalonia, Spain). Data on the environmental presence and fate of pharmaceutical metabolites and TPs is still scarce, highlighting the relevance of the developed methodology. PMID:27343613

  15. Attempt at in-air PIXE analysis of spot samples on a filter-tape mounted in an automated beta-ray absorption mass monitor

    International Nuclear Information System (INIS)

    We attempted in-air-PIXE analysis of SPM using spot samples on a filter-tape mounted in an automated beta-ray absorption mass monitor. Al, Si, S, Fe and Zn, etc., which are of interest for identifying the behavior and characteristics of SPM, were detected on the SPM spot samples on a glass-fiber filter-tape, but the peaks of these elements were nearly identical to those of blank glass-fiber filter-tape. As such, it was difficult to detect elements present in SPM from the X-ray spectra of the spot samples. On the other hand, in the case of a PTFE membrane filter-tape, the S peak was distinct and the Fe peak was also clear, and peaks for elements Al, Mn and Zn, etc., were also confirmed. Consequently, if a method for determining quantity is established, direct multi-elemental analysis by in-air-PIXE of high time-resolution SPM spot samples collected on a PTFE membrane filter-tape mounted in a SPM monitor will be possible. (author)

  16. Automated Analysis of Oxytocin by On-Line in-Tube Solid-Phase Microextraction Coupled with Liquid Chromatography-Tandem Mass Spectrometry

    Directory of Open Access Journals (Sweden)

    Eri Moriyama

    2015-06-01

    Full Text Available A simple and sensitive method for the analysis of oxytocin was developed using automated on-line in-tube solid-phase microextraction (SPME coupled with liquid chromatography-tandem mass spectrometry (LC–MS/MS. Oxytocin was separated within 3 min on a Zorbax Eclipse XDB-C8 column, with water/methanol (10/90, v/v as the mobile phase at a flow rate of 0.2 mL min−1. Electrospray ionization conditions in the positive ion mode were optimized for MS/MS detection by multiple reaction monitoring. The optimum in-tube SPME conditions were 20 draw/eject cycles of 40 µL sample at a flow rate of 250 µL min−1 using a Supel-Q PLOT capillary column as an extraction device. The extracted oxytocin was easily desorbed from the capillary by passage of the mobile phase, and no carryover was observed. The calibration curves for oxytocin were linear (r = 0.9981 in the range of 0−5.0 ng mL−1, and the relative standard deviations at each point were below 14.7% (n = 3. The limit of detection of this method was 4.0 pg mL−1, and its sensitivity was 58-fold higher than that of the direct injection method. This method was applied successfully to the analysis of oxytocin in saliva samples without any other interference peaks.

  17. Automation of High-Throughput Mass Spectrometry-Based Plasma N-Glycome Analysis with Linkage-Specific Sialic Acid Esterification.

    Science.gov (United States)

    Bladergroen, Marco R; Reiding, Karli R; Hipgrave Ederveen, Agnes L; Vreeker, Gerda C M; Clerc, Florent; Holst, Stephanie; Bondt, Albert; Wuhrer, Manfred; van der Burgt, Yuri E M

    2015-09-01

    Glycosylation is a post-translational modification of key importance with heterogeneous structural characteristics. Previously, we have developed a robust, high-throughput MALDI-TOF-MS method for the comprehensive profiling of human plasma N-glycans. In this approach, sialic acid residues are derivatized with linkage-specificity, namely the ethylation of α2,6-linked sialic acid residues with parallel lactone formation of α2,3-linked sialic acids. In the current study, this procedure was used as a starting point for the automation of all steps on a liquid-handling robot system. This resulted in a time-efficient and fully standardized procedure with throughput times of 2.5 h for a first set of 96 samples and approximately 1 h extra for each additional sample plate. The mass analysis of the thus-obtained glycans was highly reproducible in terms of relative quantification, with improved interday repeatability as compared to that of manual processing. PMID:26179816

  18. Fully automated determination of parabens, triclosan and methyl triclosan in wastewater by microextraction by packed sorbents and gas chromatography-mass spectrometry

    International Nuclear Information System (INIS)

    A fully automated method for the determination of triclosan (TCS), its derivative methyl triclosan (MeTCS) and six parabens (esters of 4-hydroxybenzoic acid) including branched and linear isomers of propyl (i-PrP and n-PrP) and butyl paraben (i-BuP and n-BuP) in sewage water samples is presented. The procedure includes analytes enrichment by microextraction by packed sorbent (MEPS) coupled at-line to large volume injection-gas chromatography-mass spectrometry (LVI-GC-MS). Under optimised conditions, compounds were extracted from 2 mL samples, adjusted at pH 3, using a C18 MEPS-sorbent. Adsorbed analytes were eluted directly into the Programmable Temperature Vaporizer (PTV) injector of the chromatograph with 2 x 25 μL of ethyl acetate. They were quantified using standard solutions in ultrapure water submitted to the same sample enrichment process as real sewage water samples. After signal normalisation using isotopic labelled species as internal surrogates, no differences were noticed among the extraction efficiency for sewage and ultrapure water; moreover, the proposed method reported lineal calibration curves from 0.1 to 10 ng mL-1, relative standard deviations (%RSD) between 2 and 7.1% and limits of detection (LODs) varying from 0.001 to 0.015 ng mL-1 in ultrapure water and from 0.02 to 0.59 ng mL-1 in the most complex sample (raw wastewater).

  19. Determination of talinolol in human plasma using automated on-line solid phase extraction combined with atmospheric pressure chemical ionization tandem mass spectrometry.

    Science.gov (United States)

    Bourgogne, Emmanuel; Grivet, Chantal; Hopfgartner, Gérard

    2005-06-01

    A specific LC-MS/MS assay was developed for the automated determination of talinolol in human plasma, using on-line solid phase extraction system (prospekt 2) combined with atmospheric pressure chemical ionization (APCI) tandem mass spectrometry. The method involved simple precipitation of plasma proteins with perchloric acid (contained propranolol) as the internal standard (IS) and injection of the supernatant onto a C8 End Capped (10 mmx2 mm) cartridge without any evaporation step. Using the back-flush mode, the analytes were transferred onto an analytical column (XTerra C18, 50 mmx4.6 mm) for chromatographic separation and mass spectrometry detection. One of the particularities of the assay is that the SPE cartridge is used as a column switching device and not as an SPE cartridge. Therefore, the same SPE cartridge could be used more than 28 times, significantly reducing the analysis cost. APCI ionization was selected to overcome any potential matrix suppression effects because the analyte and IS co-eluted. The mean precision and accuracy in the concentration range 2.5-200 ng/mL was found to be 103% and 7.4%, respectively. The data was assessed from QC samples during the validation phase of the assay. The lower limit of quantification was 2.5 ng/mL, using a 250 microL plasma aliquot. The LC-MS/MS method provided the requisite selectivity, sensitivity, robustness accuracy and precision to assess pharmacokinetics of the compound in several hundred human plasma samples. PMID:15866498

  20. Killing of targets by effector CD8 T cells in the mouse spleen follows the law of mass action

    Energy Technology Data Exchange (ETDEWEB)

    Ganusov, Vitaly V [Los Alamos National Laboratory

    2009-01-01

    In contrast with antibody-based vaccines, it has been difficult to measure the efficacy of T cell-based vaccines and to correlate the efficacy of CD8 T cell responses with protection again viral infections. In part, this difficulty is due to poor understanding of the in vivo efficacy of CD8 T cells produced by vaccination. Using a: recently developed experimental method of in vivo cytotoxicity we have investigated quantitative aspects of killing of peptide-pulsed targets by effector and memory CD8 T cells, specific to three epitopes of lymphocytic choriomeningitis virus (LCMV), in the mouse spleen. By analyzing data on killing of targets with varying number of epitope-specific effector and memory CD8 T cells, we find that killing of targets by effectors follows the law of mass-action, that is the death rate of peptide-pulsed targets is proportional to the frequency of CTLs in the spleen. In contrast, killing of targets by memory CD8 T cells does not follow the mass action law because the death rate of targets saturates at high frequencies of memory CD8 T cells. For both effector and memory cells, we also find little support for the killing term that includes the decrease of the death rate of targets with target cell density. Interestingly, our analysis suggests that at low CD8 T cell frequencies, memory CD8 T cells on the per capita basis are more efficient at killing peptide-pulsed targets than effectors, but at high frequencies, effectors are more efficient killers than memory T cells. Comparison of the estimated killing efficacy of effector T cells with the value that is predicted from theoretical physics and based on motility of T cells in lymphoid tissues, suggests that limiting step in the killing of peptide-pulsed targets is delivering the lethal hit and not finding the target. Our results thus form a basis for quantitative understanding of the process of killing of virus-infected cells by T cell responses in tissues and can be used to correlate the

  1. Mass action law versus local contagion dynamics. A mean-field statistical approach with application to the theory of epidemics

    Science.gov (United States)

    Ovidiu Vlad, Marcel; Schönfisch, Birgitt

    1996-08-01

    A mean-field approach for epidemic processes with high migration is suggested by analogy with non-equilibrium statistical mechanics. For large systems a limit of the thermodynamic type is introduced for which both the total size of the system and the total number of individuals tend to infinity but the population density remains constant. In the thermodynamic limit the infection rate is proportional to the product of the proportion of individuals susceptible to infection and the average probability of infection. The limit form of the average probability of infection is insensitive to the detailed behaviour of the fluctuations of the number of infectious individuals and may belong to two universality classes: (1) if the fluctuation of the number of infectives is non-intermittent it increases with the increase of the partial density of infectives and approaches exponentially the asymptotic value one for large densities; (2) for intermittent fluctuations obeying a power-law scaling the average probability of infection also displays a saturation effect for large densities of infectives but the asymptotic value one is approached according to a power law rather than exponentially. For low densities of infectives both expressions for the average probability of infection are linear functions of the proportion of infectives and the infection rate is given by the mass-action law.

  2. Interest Convergence or Divergence? A Critical Race Analysis of Asian Americans, Meritocracy, and Critical Mass in the Affirmative Action Debate

    Science.gov (United States)

    Park, Julie J.; Liu, Amy

    2014-01-01

    We use the Critical Race Theory frameworks of interest convergence and divergence to critique the anti-affirmative action movement's co-option of Asian Americans. Past discussions of affirmative action and Asian Americans mainly concentrate on how Asian Americans are affected by affirmative action, whether positively or negatively. We…

  3. Library Automation

    OpenAIRE

    Dhakne, B. N.; Giri, V. V; Waghmode, S. S.

    2010-01-01

    New technologies library provides several new materials, media and mode of storing and communicating the information. Library Automation reduces the drudgery of repeated manual efforts in library routine. By use of library automation collection, Storage, Administration, Processing, Preservation and communication etc.

  4. Fully automated determination of parabens, triclosan and methyl triclosan in wastewater by microextraction by packed sorbents and gas chromatography-mass spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez-Marino, Iria, E-mail: iria.gonzalez@usc.es [Department of Analytical Chemistry, Nutrition and Food Sciences, Institute for Food Analysis and Research-IIAA, University of Santiago de Compostela, 15782 Santiago de Compostela (Spain); Benito Quintana, Jose; Rodriguez, Isaac [Department of Analytical Chemistry, Nutrition and Food Sciences, Institute for Food Analysis and Research-IIAA, University of Santiago de Compostela, 15782 Santiago de Compostela (Spain); Schrader, Steffi; Moeder, Monika [Department of Analytical Chemistry, Helmholtz Centre for Environmental Research-UFZ, Permoserstrasse 15, D-04318 Leipzig (Germany)

    2011-01-17

    A fully automated method for the determination of triclosan (TCS), its derivative methyl triclosan (MeTCS) and six parabens (esters of 4-hydroxybenzoic acid) including branched and linear isomers of propyl (i-PrP and n-PrP) and butyl paraben (i-BuP and n-BuP) in sewage water samples is presented. The procedure includes analytes enrichment by microextraction by packed sorbent (MEPS) coupled at-line to large volume injection-gas chromatography-mass spectrometry (LVI-GC-MS). Under optimised conditions, compounds were extracted from 2 mL samples, adjusted at pH 3, using a C18 MEPS-sorbent. Adsorbed analytes were eluted directly into the Programmable Temperature Vaporizer (PTV) injector of the chromatograph with 2 x 25 {mu}L of ethyl acetate. They were quantified using standard solutions in ultrapure water submitted to the same sample enrichment process as real sewage water samples. After signal normalisation using isotopic labelled species as internal surrogates, no differences were noticed among the extraction efficiency for sewage and ultrapure water; moreover, the proposed method reported lineal calibration curves from 0.1 to 10 ng mL{sup -1}, relative standard deviations (%RSD) between 2 and 7.1% and limits of detection (LODs) varying from 0.001 to 0.015 ng mL{sup -1} in ultrapure water and from 0.02 to 0.59 ng mL{sup -1} in the most complex sample (raw wastewater).

  5. An online mass-based gas analyser for simultaneous determination of H2, CH4, CO, N2 and CO2: an automated sensor for process monitoring in industry

    International Nuclear Information System (INIS)

    An automated gas analyser has been designed, constructed and installed for online monitoring of H2, CH4, CO and CO2 in the reduction plant at Mobarakeh Steel Company, Iran. A small and low-resolution mass spectrometer is used in this instrument. The analyser accepts the sample directly from the ambient pressure. Mass spectra are converted to percentages of the species in the mixture based on a derived mathematical expression and especially developed software. The instrument is capable of simultaneously analyzing six different gas inputs. The instrument recorded a precision level of below 3%. (paper)

  6. ARTEMiS (Automated Robotic Terrestrial Exoplanet Microlensing Search): A possible expert-system based cooperative effort to hunt for planets of Earth mass and below

    Science.gov (United States)

    Dominik, M.; Horne, K.; Allan, A.; Rattenbury, N. J.; Tsapras, Y.; Snodgrass, C.; Bode, M. F.; Burgdorf, M. J.; Fraser, S. N.; Kerins, E.; Mottram, C. J.; Steele, I. A.; Street, R. A.; Wheatley, P. J.; Wyrzykowski, Ł.

    2008-03-01

    The technique of gravitational microlensing is currently unique in its ability to provide a sample of terrestrial exoplanets around both Galactic disk and bulge stars, allowing to measure their abundance and determine their distribution with respect to mass and orbital separation. Thus, valuable information for testing models of planet formation and orbital migration is gathered, constituting an important piece in the puzzle for the existence of life forms throughout the Universe. In order to achieve these goals in reasonable time, a well-coordinated effort involving a network of either 2m or 4×1m telescopes at each site is required. It could lead to the first detection of an Earth-mass planet outside the Solar system, and even planets less massive than Earth could be discovered. From April 2008, ARTEMiS (Automated Robotic Terrestrial Exoplanet Microlensing Search) is planned to provide a platform for a three-step strategy of survey, follow-up, and anomaly monitoring. As an expert system embedded in eSTAR (e-Science Telescopes for Astronomical Research), ARTEMiS will give advice for follow-up based on a priority algorithm that selects targets to be observed in order to maximize the expected number of planet detections, and will also alert on deviations from ordinary microlensing light curves by means of the SIGNALMEN anomaly detector. While the use of the VOEvent (Virtual Observatory Event) protocol allows a direct interaction with the telescopes that are part of the HTN (Heterogeneous Telescope Networks) consortium, additional interfaces provide means of communication with all existing microlensing campaigns that rely on human observers. The success of discovering a planet by microlensing critically depends on the availability of a telescope in a suitable location at the right time, which can mean within 10 min. To encourage follow-up observations, microlensing campaigns are therefore releasing photometric data in real time. On ongoing planetary anomalies, world

  7. A control system verifier using automated reasoning software

    International Nuclear Information System (INIS)

    An on-line, automated reasoning software system for verifying the actions of other software or human control systems has been developed. It was demonstrated by verifying the actions of an automated procedure generation system. The verifier uses an interactive theorem prover as its inference engine with the rules included as logical axioms. Operation of the verifier is generally transparent except when the verifier disagrees with the actions of the monitored software. Testing with an automated procedure generation system demonstrates the successful application of automated reasoning software for verification of logical actions in a diverse, redundant manner. A higher degree of confidence may be placed in the verified actions of the combined system

  8. Process automation

    International Nuclear Information System (INIS)

    Process automation technology has been pursued in the chemical processing industries and to a very limited extent in nuclear fuel reprocessing. Its effective use has been restricted in the past by the lack of diverse and reliable process instrumentation and the unavailability of sophisticated software designed for process control. The Integrated Equipment Test (IET) facility was developed by the Consolidated Fuel Reprocessing Program (CFRP) in part to demonstrate new concepts for control of advanced nuclear fuel reprocessing plants. A demonstration of fuel reprocessing equipment automation using advanced instrumentation and a modern, microprocessor-based control system is nearing completion in the facility. This facility provides for the synergistic testing of all chemical process features of a prototypical fuel reprocessing plant that can be attained with unirradiated uranium-bearing feed materials. The unique equipment and mission of the IET facility make it an ideal test bed for automation studies. This effort will provide for the demonstration of the plant automation concept and for the development of techniques for similar applications in a full-scale plant. A set of preliminary recommendations for implementing process automation has been compiled. Some of these concepts are not generally recognized or accepted. The automation work now under way in the IET facility should be useful to others in helping avoid costly mistakes because of the underutilization or misapplication of process automation. 6 figs

  9. Automation for tsetse mass rearing for use in sterile insect technique programmes. Final report of a co-ordinated research project 1995-2001

    International Nuclear Information System (INIS)

    The rearing of tsetse flies for the sterile insect technique has been a laborious procedure in the past. The purpose of this co-ordinated research project (CRP) 'Automation for tsetse mass rearing for use in sterile insect technique programmes' was to develop appropriate semiautomated procedures to simplify the rearing, reduce the cost and standardize the product. Two main objectives were accomplished. The first was to simplify the handling of adults at emergence. This was achieved by allowing the adults to emerge directly into the production cages. Selection of the appropriate environmental conditions and timing allowed the manipulation of the emergence pattern to achieve the desired ratio of four females to one male with minimal un-emerged females remaining mixed with the male pupae. Tests demonstrated that putting the sexes together at emergence, leaving the males in the production cages, and using a ratio of 4:1 (3:1 for a few species) did not adversely affect pupal production. This has resulted in a standardized system for the self stocking of production cages. The second was to reduce the labour involved in feeding the flies. Three distinct systems were developed and tested in sequence. The first tsetse production unit (TPU 1) was a fully automated system, but the fly survival and fecundity were unacceptably low. From this a simpler TPU 2 was developed and tested, where 63 large cages were held on a frame that could be moved as a single unit to the feeding location. TPU 2 was tested in various locations, and found to satisfy the basic requirements, and the adoption of Plexiglas pupal collection slopes resolved much of the problem due to light distribution. However the cage holding frame was heavy and difficult to position on the feeding frame and the movement disturbed the flies. TPU 2 was superseded by TPU 3, in which the cages remain stationary at all times, and the blood is brought to the flies. The blood feeding system is mounted on rails to make it

  10. Determination of aflatoxins in food samples by automated on-line in-tube solid-phase microextraction coupled with liquid chromatography-mass spectrometry.

    Science.gov (United States)

    Nonaka, Y; Saito, K; Hanioka, N; Narimatsu, S; Kataoka, H

    2009-05-15

    A simple and sensitive automated method for determination of aflatoxins (B1, B2, G1, and G2) in nuts, cereals, dried fruits, and spices was developed consisting of in-tube solid-phase microextraction (SPME) coupled with liquid chromatography-mass spectrometry (LC-MS). Aflatoxins were separated within 8 min by high-performance liquid chromatography using a Zorbax Eclipse XDB-C8 column with methanol/acetonitrile (60/40, v/v): 5mM ammonium formate (45:55) as the mobile phase. Electrospray ionization conditions in the positive ion mode were optimized for MS detection of aflatoxins. The pseudo-molecular ions [M+H](+) were used to detect aflatoxins in selected ion monitoring (SIM) mode. The optimum in-tube SPME conditions were 25draw/eject cycles of 40 microL of sample using a Supel-Q PLOT capillary column as an extraction device. The extracted aflatoxins were readily desorbed from the capillary by passage of the mobile phase, and no carryover was observed. Using the in-tube SPME LC-MS with SIM method, good linearity of the calibration curve (r>0.9994) was obtained in the concentration range of 0.05-2.0 ng/mL using aflatoxin M1 as an internal standard, and the detection limits (S/N=3) of aflatoxins were 2.1-2.8 pg/mL. The in-tube SPME method showed >23-fold higher sensitivity than the direct injection method (10 microL injection volume). The within-day and between-day precision (relative standard deviations) at the concentration of 1 ng/mL aflatoxin mixture were below 3.3% and 7.7% (n=5), respectively. This method was applied successfully to analysis of food samples without interference peaks. The recoveries of aflatoxins spiked into nuts and cereals were >80%, and the relative standard deviations were Aflatoxins were detected at <10 ng/g in several commercial food samples. PMID:19328492

  11. Analysis of betamethasone in rat plasma using automated solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry. Determination of plasma concentrations in rat following oral and intravenous administration.

    Science.gov (United States)

    Tamvakopoulos, C S; Neugebauer, J M; Donnelly, M; Griffin, P R

    2002-09-01

    A method is described for the determination of betamethasone in rat plasma by liquid chromatography-tandem mass spectrometry (LC-MS-MS). The analyte was recovered from plasma by solid-phase extraction and subsequently analyzed by LC-MS-MS. A Packard Multiprobe II, an automated liquid handling system, was employed for the preparation and extraction of a 96-well plate containing unknown plasma samples, standards and quality control samples in an automated fashion. Prednisolone, a structurally related steroid, was used as an internal standard. Using the described approach, a limit of quantitation of 2 ng/ml was achieved with a 50 microl aliquot of rat plasma. The described level of sensitivity allowed the determination of betamethasone concentrations and subsequent measurement of kinetic parameters of betamethasone in rat. Combination of automated plasma extraction and the sensitivity and selectivity of LC-MS-MS offers a valuable alternative to the methodologies currently used for the quantitation of steroids in biological fluids. PMID:12137997

  12. Universal thermodynamic model of calculating the mass action concentrations of components in a ternary strong electrolyte aqueous solution and its application in the NaCl-KCI-H2O system

    Institute of Scientific and Technical Information of China (English)

    Weijie Zhao; Hanjie Guo; Xuemin Yang; higang Dan

    2008-01-01

    A universal thermodynamic model of calculating the mass action concentrations of components in a ternary strong elec-trolyte aqueous solution has been developed based on the ion and molecule coexistence theory, and verified in the NaCl-KCl-H2Oternary system at 298.15 K, To compare the difference of the thermodynamic model in binary and ternary strong electrolyte aqueous solutions, the mass action concentrations of components in the NaCI-H20 binary strong electrolyte aqueous solution were also com-puted at 298.15K. A transformation coefficient was required to compare the calculated mass action concentration and reported activ-ity because they were obtained at different standard states and concentration units. The results show that the transformation coeffi-cients between calculated mass action concentrations and reported activities of the same components change in a very narrow range.The calculated mass action concentrations of components in the NaCl-H2O and NaCl-KCl-H2O systems are in good agreement with the reported activities. This indicates that the developed thermodynamic model can reflect the structural characteristics of solutions,and the mass action concentration also strictly follows the mass action law.

  13. Automated mass action model space generation and analysis methods for two-reactant combinatorially complex equilibriums: An analysis of ATP-induced ribonucleotide reductase R1 hexamerization data

    Directory of Open Access Journals (Sweden)

    Radivoyevitch Tomas

    2009-12-01

    Full Text Available Abstract Background Ribonucleotide reductase is the main control point of dNTP production. It has two subunits, R1, and R2 or p53R2. R1 has 5 possible catalytic site states (empty or filled with 1 of 4 NDPs, 5 possible s-site states (empty or filled with ATP, dATP, dTTP or dGTP, 3 possible a-site states (empty or filled with ATP or dATP, perhaps two possible h-site states (empty or filled with ATP, and all of this is folded into an R1 monomer-dimer-tetramer-hexamer equilibrium where R1 j-mers can be bound by variable numbers of R2 or p53R2 dimers. Trillions of RNR complexes are possible as a result. The problem is to determine which are needed in models to explain available data. This problem is intractable for 10 reactants, but it can be solved for 2 and is here for R1 and ATP. Results Thousands of ATP-induced R1 hexamerization models with up to three (s, a and h ATP binding sites per R1 subunit were automatically generated via hypotheses that complete dissociation constants are infinite and/or that binary dissociation constants are equal. To limit the model space size, it was assumed that s-sites are always filled in oligomers and never filled in monomers, and to interpret model terms it was assumed that a-sites fill before h-sites. The models were fitted to published dynamic light scattering data. As the lowest Akaike Information Criterion (AIC of the 3-parameter models was greater than the lowest of the 2-parameter models, only models with up to 3 parameters were fitted. Models with sums of squared errors less than twice the minimum were then partitioned into two groups: those that contained no occupied h-site terms (508 models and those that contained at least one (1580 models. Normalized AIC densities of these two groups of models differed significantly in favor of models that did not include an h-site term (Kolmogorov-Smirnov p -15; consistent with this, 28 of the top 30 models (ranked by AICs did not include an h-site term and 28/30 > 508/2088 with p -15. Finally, 99 of the 2088 models did not have any terms with ATP/R1 ratios >1.5, but of the top 30, there were 14 such models (14/30 > 99/2088 with p -16, i.e. the existence of R1 hexamers with >3 a-sites occupied by ATP is also not supported by this dataset. Conclusion The analysis presented suggests that three a-sites may not be occupied by ATP in R1 hexamers under the conditions of the data analyzed. If a-sites fill before h-sites, this implies that the dataset analyzed can be explained without the existence of an h-site. Reviewers This article was reviewed by Ossama Kashlan (nominated by Philip Hahnfeldt, Bin Hu (nominated by William Hlavacek and Rainer Sachs.

  14. Heat and Mass Exchange of a Drop of a Solution Subjected to a Combined Energy Action Under Conditions of Deepening of the Evaporation Zone

    Science.gov (United States)

    Akulich, P. V.

    2016-06-01

    A model of the heat and mass exchange in the evaporation of drops moving in the carrying gas phase of a solution subjected to the combined energy action of a convective heat flow and electromagnetic infrared and microwave radiations under conditions where the size of the drops changes at the low-temperature stage of their evaporation and the evaporation zone deepens at the high-temperature stage of the evaporation process has been formulated. Kinetic dependences and features of the movement of the evaporation boundary in the process of dehydration of the indicated drops have been determined.

  15. Automation Security

    OpenAIRE

    Mirzoev, Dr. Timur

    2014-01-01

    Web-based Automated Process Control systems are a new type of applications that use the Internet to control industrial processes with the access to the real-time data. Supervisory control and data acquisition (SCADA) networks contain computers and applications that perform key functions in providing essential services and commodities (e.g., electricity, natural gas, gasoline, water, waste treatment, transportation) to all Americans. As such, they are part of the nation s critical infrastructu...

  16. An automated, open-source pipeline for mass production of digital elevation models (DEMs) from very-high-resolution commercial stereo satellite imagery

    Science.gov (United States)

    Shean, David E.; Alexandrov, Oleg; Moratto, Zachary M.; Smith, Benjamin E.; Joughin, Ian R.; Porter, Claire; Morin, Paul

    2016-06-01

    We adapted the automated, open source NASA Ames Stereo Pipeline (ASP) to generate digital elevation models (DEMs) and orthoimages from very-high-resolution (VHR) commercial imagery of the Earth. These modifications include support for rigorous and rational polynomial coefficient (RPC) sensor models, sensor geometry correction, bundle adjustment, point cloud co-registration, and significant improvements to the ASP code base. We outline a processing workflow for ∼0.5 m ground sample distance (GSD) DigitalGlobe WorldView-1 and WorldView-2 along-track stereo image data, with an overview of ASP capabilities, an evaluation of ASP correlator options, benchmark test results, and two case studies of DEM accuracy. Output DEM products are posted at ∼2 m with direct geolocation accuracy of automated iterative closest-point (ICP) co-registration tool reduces absolute vertical and horizontal error to <0.5 m where appropriate ground-control data are available, with observed standard deviation of ∼0.1-0.5 m for overlapping, co-registered DEMs (n = 14, 17). While ASP can be used to process individual stereo pairs on a local workstation, the methods presented here were developed for large-scale batch processing in a high-performance computing environment. We are leveraging these resources to produce dense time series and regional mosaics for the Earth's polar regions.

  17. Automated digital image analysis of islet cell mass using Nikon's inverted eclipse Ti microscope and software to improve engraftment may help to advance the therapeutic efficacy and accessibility of islet transplantation across centers.

    Science.gov (United States)

    Gmyr, Valery; Bonner, Caroline; Lukowiak, Bruno; Pawlowski, Valerie; Dellaleau, Nathalie; Belaich, Sandrine; Aluka, Isanga; Moermann, Ericka; Thevenet, Julien; Ezzouaoui, Rimed; Queniat, Gurvan; Pattou, Francois; Kerr-Conte, Julie

    2015-01-01

    Reliable assessment of islet viability, mass, and purity must be met prior to transplanting an islet preparation into patients with type 1 diabetes. The standard method for quantifying human islet preparations is by direct microscopic analysis of dithizone-stained islet samples, but this technique may be susceptible to inter-/intraobserver variability, which may induce false positive/negative islet counts. Here we describe a simple, reliable, automated digital image analysis (ADIA) technique for accurately quantifying islets into total islet number, islet equivalent number (IEQ), and islet purity before islet transplantation. Islets were isolated and purified from n = 42 human pancreata according to the automated method of Ricordi et al. For each preparation, three islet samples were stained with dithizone and expressed as IEQ number. Islets were analyzed manually by microscopy or automatically quantified using Nikon's inverted Eclipse Ti microscope with built-in NIS-Elements Advanced Research (AR) software. The AIDA method significantly enhanced the number of islet preparations eligible for engraftment compared to the standard manual method (p methods showed good correlations between mean values of IEQ number (r(2) = 0.91) and total islet number (r(2) = 0.88) and thus increased to r(2) = 0.93 when islet surface area was estimated comparatively with IEQ number. The ADIA method showed very high intraobserver reproducibility compared to the standard manual method (p method versus the ADIA method (p method also detected small islets between 10 and 50 µm in size. Automated digital image analysis utilizing the Nikon Instruments software is an unbiased, simple, and reliable teaching tool to comprehensively assess the individual size of each islet cell preparation prior to transplantation. Implementation of this technology to improve engraftment may help to advance the therapeutic efficacy and accessibility of islet transplantation across centers. PMID:23683575

  18. Quantification of 31 illicit and medicinal drugs and metabolites in whole blood by fully automated solid-phase extraction and ultra-performance liquid chromatography-tandem mass spectrometry.

    Science.gov (United States)

    Bjørk, Marie Kjærgaard; Simonsen, Kirsten Wiese; Andersen, David Wederkinck; Dalsgaard, Petur Weihe; Sigurðardóttir, Stella Rögn; Linnet, Kristian; Rasmussen, Brian Schou

    2013-03-01

    An efficient method for analyzing illegal and medicinal drugs in whole blood using fully automated sample preparation and short ultra-high-performance liquid chromatography-tandem mass spectrometry (MS/MS) run time is presented. A selection of 31 drugs, including amphetamines, cocaine, opioids, and benzodiazepines, was used. In order to increase the efficiency of routine analysis, a robotic system based on automated liquid handling and capable of handling all unit operation for sample preparation was built on a Freedom Evo 200 platform with several add-ons from Tecan and third-party vendors. Solid-phase extraction was performed using Strata X-C plates. Extraction time for 96 samples was less than 3 h. Chromatography was performed using an ACQUITY UPLC system (Waters Corporation, Milford, USA). Analytes were separated on a 100 mm × 2.1 mm, 1.7 μm Acquity UPLC CSH C(18) column using a 6.5 min 0.1 % ammonia (25 %) in water/0.1 % ammonia (25 %) in methanol gradient and quantified by MS/MS (Waters Quattro Premier XE) in multiple-reaction monitoring mode. Full validation, including linearity, precision and trueness, matrix effect, ion suppression/enhancement of co-eluting analytes, recovery, and specificity, was performed. The method was employed successfully in the laboratory and used for routine analysis of forensic material. In combination with tetrahydrocannabinol analysis, the method covered 96 % of cases involving driving under the influence of drugs. The manual labor involved in preparing blood samples, solvents, etc., was reduced to a half an hour per batch. The automated sample preparation setup also minimized human exposure to hazardous materials, provided highly improved ergonomics, and eliminated manual pipetting. PMID:23292043

  19. Vaccination rules for a true-mass action SEIR epidemic model based on an observer synthesis. Preliminary results

    CERN Document Server

    De la Sen, M; Alonso-Quesada, S

    2011-01-01

    This paper presents a simple continuous-time linear vaccination-based control strategy for a SEIR (susceptible plus infected plus infectious plus removed populations) propagation disease model. The model takes into account the total population amounts as a refrain for the illness transmission since its increase makes more difficult contacts among susceptible and infected. The control objective is the asymptotically tracking of the removed-byimmunity population to the total population while achieving simultaneously the remaining population (i.e. susceptible plus infected plus infectious) to asymptotically converge to zero. A state observer is used to estimate the true various partial populations of susceptible, infected, infectious and immune which are assumed to be unknown. The model parameters are also assumed to be, in general, unknown. In this case, the parameters are replaced by available estimates to implement the vaccination action.

  20. Murine Automated Urine Sampler (MAUS) Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This proposal outlines planned development for a low-power, low-mass automated urine sample collection and preservation system for small mammals, capable of...

  1. Automated generation of lattice QCD Feynman rules

    Energy Technology Data Exchange (ETDEWEB)

    Hart, A.; Mueller, E.H. [Edinburgh Univ. (United Kingdom). SUPA School of Physics and Astronomy; von Hippel, G.M. [Deutsches Elektronen-Synchrotron (DESY), Zeuthen (Germany); Horgan, R.R. [Cambridge Univ. (United Kingdom). DAMTP, CMS

    2009-04-15

    The derivation of the Feynman rules for lattice perturbation theory from actions and operators is complicated, especially for highly improved actions such as HISQ. This task is, however, both important and particularly suitable for automation. We describe a suite of software to generate and evaluate Feynman rules for a wide range of lattice field theories with gluons and (relativistic and/or heavy) quarks. Our programs are capable of dealing with actions as complicated as (m)NRQCD and HISQ. Automated differentiation methods are used to calculate also the derivatives of Feynman diagrams. (orig.)

  2. Automated generation of lattice QCD Feynman rules

    International Nuclear Information System (INIS)

    The derivation of the Feynman rules for lattice perturbation theory from actions and operators is complicated, especially for highly improved actions such as HISQ. This task is, however, both important and particularly suitable for automation. We describe a suite of software to generate and evaluate Feynman rules for a wide range of lattice field theories with gluons and (relativistic and/or heavy) quarks. Our programs are capable of dealing with actions as complicated as (m)NRQCD and HISQ. Automated differentiation methods are used to calculate also the derivatives of Feynman diagrams. (orig.)

  3. Validation of an automated solid-phase extraction method for the analysis of 23 opioids, cocaine, and metabolites in urine with ultra-performance liquid chromatography-tandem mass spectrometry.

    Science.gov (United States)

    Ramírez Fernández, María del Mar; Van Durme, Filip; Wille, Sarah M R; di Fazio, Vincent; Kummer, Natalie; Samyn, Nele

    2014-06-01

    The aim of this work was to automate a sample preparation procedure extracting morphine, hydromorphone, oxymorphone, norcodeine, codeine, dihydrocodeine, oxycodone, 6-monoacetyl-morphine, hydrocodone, ethylmorphine, benzoylecgonine, cocaine, cocaethylene, tramadol, meperidine, pentazocine, fentanyl, norfentanyl, buprenorphine, norbuprenorphine, propoxyphene, methadone and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine from urine samples. Samples were extracted by solid-phase extraction (SPE) with cation exchange cartridges using a TECAN Freedom Evo 100 base robotic system, including a hydrolysis step previous extraction when required. Block modules were carefully selected in order to use the same consumable material as in manual procedures to reduce cost and/or manual sample transfers. Moreover, the present configuration included pressure monitoring pipetting increasing pipetting accuracy and detecting sampling errors. The compounds were then separated in a chromatographic run of 9 min using a BEH Phenyl analytical column on a ultra-performance liquid chromatography-tandem mass spectrometry system. Optimization of the SPE was performed with different wash conditions and elution solvents. Intra- and inter-day relative standard deviations (RSDs) were within ±15% and bias was within ±15% for most of the compounds. Recovery was >69% (RSD heroin, buprenorphine and methadone, offering fast and reliable results. Automation resulted in improved precision and accuracy, and a minimum operator intervention, leading to safer sample handling and less time-consuming procedures. PMID:24790061

  4. An automated delayed neutron counting system for mass determination of fissile isotopes in special nuclear materials at the Royal Military College of Canada

    International Nuclear Information System (INIS)

    A Delayed Neutron Counting System (DNCS) has been designed and installed to enhance the ability of Royal Military College of Canada (RMC) to analyze Special Nuclear Materials (SNM). By detecting and recording delayed neutron counts from SNM samples irradiated by the SLOWPOKE-2 Reactor Facility at RMC, the DNCS is able to determine the masses of multiple fissile nuclei in the samples efficiently and accurately. The system is controlled by LabVIEW software. A separate mathematical program has also been developed to determine the mass of fissile nuclei present in SNM. The DNCS and fissile mass determination program have been validated using powdered UO2 samples. (author)

  5. Quantification of 31 illicit and medicinal drugs and metabolites in whole blood by fully automated solid-phase extraction and ultra-performance liquid chromatography-tandem mass spectrometry

    DEFF Research Database (Denmark)

    Bjørk, Marie Kjærgaard; Simonsen, Kirsten Wiese; Andersen, David Wederkinck;

    2013-01-01

    An efficient method for analyzing illegal and medicinal drugs in whole blood using fully automated sample preparation and short ultra-high-performance liquid chromatography–tandem mass spectrometry (MS/MS) run time is presented. A selection of 31 drugs, including amphetamines, cocaine, opioids......, was performed. The method was employed successfully in the laboratory and used for routine analysis of forensic material. In combination with tetrahydrocannabinol analysis, the method covered 96 % of cases involving driving under the influence of drugs. The manual labor involved in preparing blood samples......-phase extraction was performed using Strata X-C plates. Extraction time for 96 samples was less than 3 h. Chromatography was performed using an ACQUITY UPLC system (Waters Corporation, Milford, USA). Analytes were separated on a 100 mm × 2.1 mm, 1.7 μm Acquity UPLC CSH C18 column using a 6.5 min 0.1 % ammonia (25...

  6. Automated Budget System

    Data.gov (United States)

    Department of Transportation — The Automated Budget System (ABS) automates management and planning of the Mike Monroney Aeronautical Center (MMAC) budget by providing enhanced capability to plan,...

  7. Choline in infant formula and adult/pediatric nutritional Formula by ultra high-performance liquid chromatography/ Tandem mass spectrometry: AOAC First Action 2012.18.

    Science.gov (United States)

    Martin, Frederic; Gimenez, Catherine; Fontannaz, Patric; Kilinc, Tamara; Campos-Giménez, Esther; Dowell, Dawn

    2013-01-01

    The method described below is for the determination of choline in infant formula and adult/pediatric nutritional formula by ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The single-laboratory validation data were submitted to the Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) Expert Review Panel (ERP) for review at the AOAC INTERNATIONAL Annual Meeting held September 30 to October 3, 2012 in Las Vegas, NV. The ERP determined that the data reviewed met the standard method performance requirements set by SPIFAN, and the method was approved as AOAC Official First Action. The analytical range was found to be between 0.16 and 3.2 microg/mL. The recovery rates were within 80-120% at 50 and 100% of native levels for all samples. Repeatability precision (RSDr) was < 3%, with intermediate reproducibility (RSDir) no higher than 4%. PMID:24645520

  8. Non-perturbative renormalization in coordinate space for N{sub f}=2 maximally twisted mass fermions with tree-level Symanzik improved gauge action

    Energy Technology Data Exchange (ETDEWEB)

    Cichy, Krzysztof, E-mail: krzysztof.cichy@desy.de [NIC, DESY, Platanenallee 6, D-15738 Zeuthen (Germany); Adam Mickiewicz University, Faculty of Physics, Umultowska 85, 61-614 Poznan (Poland); Jansen, Karl, E-mail: karl.jansen@desy.de [NIC, DESY, Platanenallee 6, D-15738 Zeuthen (Germany); Korcyl, Piotr, E-mail: piotr.korcyl@desy.de [NIC, DESY, Platanenallee 6, D-15738 Zeuthen (Germany); M. Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Krakow (Poland)

    2012-12-11

    We present results of a Lattice QCD application of a coordinate space renormalization scheme for the extraction of renormalization constants for flavour non-singlet bilinear quark operators. The method consists in the analysis of the small-distance behaviour of correlation functions in Euclidean space and has several theoretical and practical advantages, in particular: it is gauge invariant, easy to implement and has relatively low computational cost. The values of renormalization constants in the X-space scheme can be converted to the MS{sup Macron} scheme via 4-loop continuum perturbative formulae. Our results for N{sub f}=2 maximally twisted mass fermions with tree-level Symanzik improved gauge action are compared to the ones from the RI-MOM scheme and show full agreement with this method.

  9. Ab initio free energy of vacancy formation and mass-action kinetics in vis-active TiO{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Keith, J Brandon [Department of Physics, West Virginia University, PO Box 6315, 209 Hodges Hall, Morgantown, WV 26506 (United States); Wang Hao [Department of Physics, West Virginia University, PO Box 6315, 209 Hodges Hall, Morgantown, WV 26506 (United States); Fultz, Brent [California Institute of Technology, Division of Engineering and Applied Science, Mail Code 138-78, Pasadena, CA 91125 (United States); Lewis, James P [Department of Physics, West Virginia University, PO Box 6315, 209 Hodges Hall, Morgantown, WV 26506 (United States)

    2008-01-16

    Recent reports have identified bulk defects such as oxygen vacancies as key players in visible-light photoactive TiO{sub 2}. This would imply greater visible light absorption rates may be possible provided effective defect engineering can be achieved. To further this we have developed methods to simulate vacancy formation in bulk TiO{sub 2} using ab initio techniques. Initial results of these methods show an entropic reduction in the free energy of vacancy formation of 2.3 eV over a range of 266 K. The use of this result is illustrated by a 'toy' mass-action kinetics model which offers insight into vacancy concentration, rate constants, and enthalpy of reaction. (fast track communication)

  10. Non-perturbative renormalization in coordinate space for N{sub f}=2 maximally twisted mass fermions with tree-level Symanzik improved gauge action

    Energy Technology Data Exchange (ETDEWEB)

    Cichy, Krzysztof [DESY, Zeuthen (Germany). NIC; Adam Mickiewicz Univ., Poznan (Poland). Faculty of Physics; Jansen, Karl [DESY, Zeuthen (Germany). NIC; Korcyl, Piotr [DESY, Zeuthen (Germany). NIC; Jagiellonian Univ., Krakow (Poland). M. Smoluchowski Inst. of Physics

    2012-07-15

    We present results of a lattice QCD application of a coordinate space renormalization scheme for the extraction of renormalization constants for flavour non-singlet bilinear quark operators. The method consists in the analysis of the small-distance behaviour of correlation functions in Euclidean space and has several theoretical and practical advantages, in particular: it is gauge invariant, easy to implement and has relatively low computational cost. The values of renormalization constants in the X-space scheme can be converted to the MS scheme via 4-loop continuum perturbative formulae. Our results for N{sub f}=2 maximally twisted mass fermions with tree-level Symanzik improved gauge action are compared to the ones from the RI-MOM scheme and show full agreement with this method. (orig.)

  11. Non-perturbative renormalization in coordinate space for $N_f=2$ maximally twisted mass fermions with tree-level Symanzik improved gauge action

    CERN Document Server

    Cichy, Krzysztof; Korcyl, Piotr

    2012-01-01

    We present results of a lattice QCD application of a coordinate space renormalization scheme for the extraction of renormalization constants for flavour non-singlet bilinear quark operators. The method consists in the analysis of the small-distance behaviour of correlation functions in Euclidean space and has several theoretical and practical advantages, in particular: it is gauge invariant, easy to implement and has relatively low computational cost. The values of renormalization constants in the X-space scheme can be converted to the MSbar scheme via 4-loop continuum perturbative formulae. Our results for $N_f=2$ maximally twisted mass fermions with tree-level Symanzik improved gauge action are compared to the ones from the RI-MOM scheme and show full agreement with this method.

  12. Action spectroscopy of SrCl$^+$ using an integrated ion trap time-of-flight mass spectrometer

    CERN Document Server

    Puri, Prateek; Kotochigova, Svetlana; Petrov, Alexander; Hudson, Eric R

    2014-01-01

    The photodissociation cross-section of SrCl$^+$ is measured in the spectral range of 36000 -- 46000 cm$^{-1}$ using a modular time-of-flight mass spectrometer (TOF-MS). By irradiating a sample of trapped SrCl$^+$ molecular ions with a pulsed dye laser, X$^1\\Sigma^+$ state molecular ions are electronically excited to the repulsive wall of the A$^1\\Pi$ state, resulting in dissociation. Using the TOF-MS, the fragments are detected and the photodissociation cross-section is determined for a broad range of photon energies. Detailed $\\textit{ab initio}$ calculations of the molecular potentials and spectroscopic constants are also performed and are found to be in good agreement with experiment. The spectroscopic constants for SrCl$^+$ are also compared to those of another alkaline earth chalcogen, BaCl$^+$, in order to highlight structural differences between the two molecular ions. This work represents the first spectroscopy and $\\textit{ab initio}$ calculations of SrCl$^+$.

  13. Action spectroscopy of SrCl+ using an integrated ion trap time-of-flight mass spectrometer

    International Nuclear Information System (INIS)

    The photodissociation cross-section of SrCl+ is measured in the spectral range of 36 000–46 000 cm−1 using a modular time-of-flight mass spectrometer (TOF-MS). By irradiating a sample of trapped SrCl+ molecular ions with a pulsed dye laser, X1Σ+ state molecular ions are electronically excited to the repulsive wall of the A1Π state, resulting in dissociation. Using the TOF-MS, the product fragments are detected and the photodissociation cross-section is determined for a broad range of photon energies. Detailed ab initio calculations of the SrCl+ molecular potentials and spectroscopic constants are also performed and are found to be in good agreement with experiment. The spectroscopic constants for SrCl+ are also compared to those of another alkaline earth halogen, BaCl+, in order to highlight structural differences between the two molecular ions. This work represents the first spectroscopy and ab initio calculations of SrCl+

  14. Action spectroscopy of SrCl{sup +} using an integrated ion trap time-of-flight mass spectrometer

    Energy Technology Data Exchange (ETDEWEB)

    Puri, Prateek, E-mail: teek24@ucla.edu; Schowalter, Steven J.; Hudson, Eric R. [Department of Physics and Astronomy, University of California, Los Angeles, California 90095 (United States); Kotochigova, Svetlana; Petrov, Alexander [Department of Physics, Temple University, Philadelphia, Pennsylvania 19122 (United States)

    2014-07-07

    The photodissociation cross-section of SrCl{sup +} is measured in the spectral range of 36 000–46 000 cm{sup −1} using a modular time-of-flight mass spectrometer (TOF-MS). By irradiating a sample of trapped SrCl{sup +} molecular ions with a pulsed dye laser, X{sup 1}Σ{sup +} state molecular ions are electronically excited to the repulsive wall of the A{sup 1}Π state, resulting in dissociation. Using the TOF-MS, the product fragments are detected and the photodissociation cross-section is determined for a broad range of photon energies. Detailed ab initio calculations of the SrCl{sup +} molecular potentials and spectroscopic constants are also performed and are found to be in good agreement with experiment. The spectroscopic constants for SrCl{sup +} are also compared to those of another alkaline earth halogen, BaCl{sup +}, in order to highlight structural differences between the two molecular ions. This work represents the first spectroscopy and ab initio calculations of SrCl{sup +}.

  15. Increasing productivity for the analysis of trace contaminants in food by gas chromatography-mass spectrometry using automated liner exchange, backflushing and heart-cutting.

    Science.gov (United States)

    David, Frank; Tienpont, Bart; Devos, Christophe; Lerch, Oliver; Sandra, Pat

    2013-10-25

    Laboratories focusing on residue analysis in food are continuously seeking to increase sample throughput by minimizing sample preparation. Generic sample extraction methods such as QuEChERS lack selectivity and consequently extracts are not free from non-volatile material that contaminates the analytical system. Co-extracted matrix constituents interfere with target analytes, even if highly sensitive and selective GC-MS/MS is used. A number of GC approaches are described that can be used to increase laboratory productivity. These techniques include automated inlet liner exchange and column backflushing for preservation of the performance of the analytical system and heart-cutting two-dimensional GC for increasing sensitivity and selectivity. The application of these tools is illustrated by the analysis of pesticides in vegetables and fruits, PCBs in milk powder and coplanar PCBs in fish. It is demonstrated that considerable increase in productivity can be achieved by decreasing instrument down-time, while analytical performance is equal or better compared to conventional trace contaminant analysis. PMID:23891373

  16. Automated method for the direct analysis of 8-oxo-guanosine and 8-oxo-2 '-deoxyguanosine in human urine using ultraperformance liquid chromatography and tandem mass spectrometry

    DEFF Research Database (Denmark)

    Henriksen, T.; Hillestrom, P.R.; Poulsen, Henrik Enghusen; Weimann, A.

    2009-01-01

    addition there is a need for assays that can measure more than one product from DNA oxidation. We present a sensitive, precise, and accurate method for quantitative analysis of the oxidized nucleosides 8-oxoGuo and 8-oxodG in human urine. The assay is based on automated sample handling using a BIOMEK 3000......The potential use of oxidative stress-induced DNA and RNA damage products as biomarkers is an important aspect of biomedical research. There is a need for assays with high specificity and sensitivity that also can be used in molecular epidemiology studies with a large number of subjects. In...... Workstation, and UPLC-ESI(+)-MS/MS analysis. High specificity is evidenced by the use of qualifier ions for both analytes. The quantification limit in urine samples is 1 nM for both analytes. Accuracy and precision were documented, showing average recoveries of 106.2% (8-oxoGuo) and 106.9% (8-oxodG), and...

  17. Automated method for the direct analysis of 8-oxo-guanosine and 8-oxo-2'-deoxyguanosine in human urine using ultraperformance liquid chromatography and tandem mass spectrometry

    DEFF Research Database (Denmark)

    Hillestrøm, Peter R; Henriksen, Trine; Hillestrøm, Peter René; Poulsen, Henrik E; Weimann, Allan

    2009-01-01

    addition there is a need for assays that can measure more than one product from DNA oxidation. We present a sensitive, precise, and accurate method for quantitative analysis of the oxidized nucleosides 8-oxoGuo and 8-oxodG in human urine. The assay is based on automated sample handling using a BIOMEK 3000......The potential use of oxidative stress-induced DNA and RNA damage products as biomarkers is an important aspect of biomedical research. There is a need for assays with high specificity and sensitivity that also can be used in molecular epidemiology studies with a large number of subjects. In...... Workstation, and UPLC-ESI(+)-MS/MS analysis. High specificity is evidenced by the use of qualifier ions for both analytes. The quantification limit in urine samples is 1 nM for both analytes. Accuracy and precision were documented, showing average recoveries of 106.2% (8-oxoGuo) and 106.9% (8-oxodG), and...

  18. Automated N-glycan profiling of a mutant Trypanosoma rangeli sialidase expressed in Pichia pastoris, using tandem mass spectrometry and bioinformatics

    DEFF Research Database (Denmark)

    Li, Haiying; Rasmussen, Morten I; Larsen, Martin R;

    2015-01-01

    A mutant Trypanosoma rangeli sialidase, Tr7, expressed in Pichia pastoris, exhibits significant trans-sialidase activity, and has been used for analytical-scale production of sialylated human milk oligosaccharides. Mass spectrometry-based site-specific N-glycoprofiling of Tr7 showed that heteroge...

  19. On Saturn's rotation relative to a center of mass under the action of the gravitational moments of the Sun and Jupiter

    Science.gov (United States)

    Krasilnikov, P. S.; Amelin, R. N.

    2016-03-01

    Saturn's rotation relative to a center of mass is considered within an elliptic restricted three-body problem. It is assumed that Saturn is a solid under the action of gravity of the Sun and Jupiter. The motions of Saturn and Jupiter are considered elliptic with small eccentricities e S and e J , respectively; the mean motion of Jupiter n J is also small. We obtain the averaged Hamiltonian function for a small parameter of ɛ = n J and integrals of evolution equations. The main effects of the influence of Jupiter on Saturn's rotation are described: (α) the evolution of the constant parameters of regular precession for the angular momentum vector I2; (β) the occurrence of new libration zones of oscillations I2 near the plane of the celestial equator parallel to the plane of the Jupiter's orbit; (γ) the occurrence of additional unstable equilibria of vector I2 at the points of the north and south poles of the celestial sphere and, as a result, the existence of homoclinic trajectories; and (δ) the existence of periodic trajectories with arbitrarily large periods near the homoclinic trajectory. It is shown that the effects of (β), (γ), and (δ) are caused by the eccentricity e of the Jupiter's orbit and are practically independent of Jupiter's mass (within satellite approximation).

  20. Automation from pictures

    International Nuclear Information System (INIS)

    The state transition diagram (STD) model has been helpful in the design of real time software, especially with the emergence of graphical computer aided software engineering (CASE) tools. Nevertheless, the translation of the STD to real time code has in the past been primarily a manual task. At Los Alamos we have automated this process. The designer constructs the STD using a CASE tool (Cadre Teamwork) using a special notation for events and actions. A translator converts the STD into an intermediate state notation language (SNL), and this SNL is compiled directly into C code (a state program). Execution of the state program is driven by external events, allowing multiple state programs to effectively share the resources of the host processor. Since the design and the code are tightly integrated through the CASE tool, the design and code never diverge, and we avoid design obsolescence. Furthermore, the CASE tool automates the production of formal technical documents from the graphic description encapsulated by the CASE tool. (author)

  1. Automated digital magnetofluidics

    Energy Technology Data Exchange (ETDEWEB)

    Schneider, J; Garcia, A A; Marquez, M [Harrington Department of Bioengineering Arizona State University, Tempe AZ 85287-9709 (United States)], E-mail: tony.garcia@asu.edu

    2008-08-15

    Drops can be moved in complex patterns on superhydrophobic surfaces using a reconfigured computer-controlled x-y metrology stage with a high degree of accuracy, flexibility, and reconfigurability. The stage employs a DMC-4030 controller which has a RISC-based, clock multiplying processor with DSP functions, accepting encoder inputs up to 22 MHz, provides servo update rates as high as 32 kHz, and processes commands at rates as fast as 40 milliseconds. A 6.35 mm diameter cylindrical NdFeB magnet is translated by the stage causing water drops to move by the action of induced magnetization of coated iron microspheres that remain in the drop and are attracted to the rare earth magnet through digital magnetofluidics. Water drops are easily moved in complex patterns in automated digital magnetofluidics at an average speed of 2.8 cm/s over a superhydrophobic polyethylene surface created by solvent casting. With additional components, some potential uses for this automated microfluidic system include characterization of superhydrophobic surfaces, water quality analysis, and medical diagnostics.

  2. Automated and high confidence protein phosphorylation site localization using complementary collision-activated dissociation and electron transfer dissociation tandem mass spectrometry

    DEFF Research Database (Denmark)

    Hansen, Thomas A; Sylvester, Marc; Jensen, Ole N;

    2012-01-01

    Reversible protein phosphorylation plays a critical role in cell signaling and is responsible for the regulation of many biological processes in most living organisms. The low stoichiometry of protein phosphorylation requires sensitive analysis by tandem mass spectrometry. However, incomplete...... peptide fragmentation and the loss of labile phosphate groups complicate identification of the site of the phosphate motif. Here, we have implemented and evaluated a novel approach for phospho-site localization by the combined use of peptide tandem mass spectrometry data obtained using both collision......-site localization and the number of assigned phospho-sites at a fixed false-localization rate. The average calculated Cscore from a large data set (>7000 phosphopeptide MS/MS spectra) was ∼32 compared to ∼23 and ∼17 for the Ascore using collision-activated dissociation (CAD) or electron transfer dissociation (ETD...

  3. Manufacturing and automation

    OpenAIRE

    Ernesto Córdoba Nieto

    2010-01-01

    The article presents concepts and definitions from different sources concerning automation. The work approaches automation by virtue of the author’s experience in manufacturing production; why and how automation prolects are embarked upon is considered. Technological reflection regarding the progressive advances or stages of automation in the production area is stressed. Coriat and Freyssenet’s thoughts about and approaches to the problem of automation and its current state are taken and e...

  4. Process development for automated solar cell and module production. Task 4: automated array assembly

    Energy Technology Data Exchange (ETDEWEB)

    Hagerty, J.J.

    1980-06-30

    The scope of work under this contract involves specifying a process sequence which can be used in conjunction with automated equipment for the mass production of solar cell modules for terrestrial use. This process sequence is then critically analyzed from a technical and economic standpoint to determine the technological readiness of each process step for implementation. The process steps are ranked according to the degree of development effort required and according to their significance to the overall process. Under this contract the steps receiving analysis were: back contact metallization, automated cell array layup/interconnect, and module edge sealing. For automated layup/interconnect both hard automation and programmable automation (using an industrial robot) were studied. The programmable automation system was then selected for actual hardware development. Economic analysis using the SAMICS system has been performed during these studies to assure that development efforts have been directed towards the ultimate goal of price reduction. Details are given. (WHK)

  5. 使用自动解卷积技术鉴别西江水中有机污染物%Identification of Organic Pollutants in West River by Automated Mass Spectral Deconvolution

    Institute of Scientific and Technical Information of China (English)

    曾锦明; 苏宇亮

    2012-01-01

    Identification of organic pollutants in West River of China by automated mass spectral deconvolution was introduced.The mass spectral deconvolution was on the basis of optimized "model peak" approach,able to extract "pure" mass spectra of components from complex chromatograms and lock the retention time.Also it could search libraries,calculated "match factors" by comparing component spectrum with library,and automatically identified the target compounds.The results showed that West River contained priority controlled pollutants in water environment by the United State Environment Protection Agency(USEPA).Besides,pollutants which from plastics industry,pesticides,drugs,pharmaceutical and personal care products(PPCPs) should be highly concerned.%文章使用自动解卷积技术对西江水体中有机污染物进行了鉴定。解卷积技术基于"模型峰"法,能够从复杂的色谱中提取组份的"纯"质谱,并进行保留时间锁定,数据库检索和比较,最后根据质谱的匹配因子自动作出鉴定。结果显示西江水中含有多种美国环保局水环境中优先控制污染物,此外,塑料工业产生的污染物、农药、药物和个人护理品(PPCPs)应被高度关注。

  6. Automated headspace solid-phase microextraction and on-fiber derivatization for the determination of clenbuterol in meat products by gas chromatography coupled to mass spectrometry.

    Science.gov (United States)

    Jiang, Yong; Ni, Yongnian

    2015-02-01

    A method was developed for the determination of clenbuterol in meat using stable-isotope-dilution gas chromatography with mass spectrometry coupled with solid-phase microextraction and on-fiber derivatization. The samples were first homogenized with hydrochloric acid followed by protein deposition. After headspace solid-phase microextraction and on-fiber derivatization, the content of clenbuterol was measured with the aid of stable-isotope dilution. The condition of solid-phase microextraction was optimized by central composite design. The relative standard deviations, limit of detection, and recoveries for clenbuterol were 4.2-9.2%, 0.48 μg/kg, and 96-104%, respectively. The proposed method was satisfactory for analysis of real samples as compared with the Chinese standard method. PMID:25403577

  7. Sense of Control in Supervision Tasks of Automated Systems.

    OpenAIRE

    Berberian, B.; Le Blaye, P.; Maille, N.; Sarrazin, J. C.

    2012-01-01

    We live in an increasingly technological world. Automated systems certainly can make life easier, but they can also create complexity and uncertainty. Moreover, it is clear that automation does not merely supplant human activity, but also transforms the nature of human work. This review examines an original account of this transformation – a link between automation technology and the sense that our actions cause effects on the outside world (so-called ‘agency’). Accordingly, we first discuss ...

  8. COMMON PROBLEMS OF DECISION SUPPORT SYSTEMS IN INDUSTRIAL AUTOMATION

    OpenAIRE

    Бідна Лідія Андріївна

    2015-01-01

    At this stage of governance, many managers are turning to automatic and automated control systems.  Such systems provide a more efficient and systematic approach to solving complex, systemic issues. Now actively use automated systems management in IT companies, as these companies combine a lot of automated processes with important management decisions. If we consider avtamatychne management technique, it is a set of actions aimed at maintaining or improving the functioning of the faci...

  9. Determination of thermodynamic potentials and the aggregation number for micelles with the mass-action model by isothermal titration calorimetry: A case study on bile salts.

    Science.gov (United States)

    Olesen, Niels Erik; Westh, Peter; Holm, René

    2015-09-01

    The aggregation number (n), thermodynamic potentials (ΔG, ΔH, ΔS) and critical micelle concentration (CMC) for 6 natural bile salts were determined on the basis of both original and previously published isothermal titration calorimetry (ITC) data. Different procedures to estimate parameters of micelles with ITC were compared to a mass-action model (MAM) of reaction type: n⋅S⇌Mn. This analysis can provide guidelines for future ITC studies of systems behaving in accordance with this model such as micelles and proteins that undergo self-association to oligomers. Micelles with small aggregation numbers, as those of bile salts, are interesting because such small aggregates cannot be characterized as a separate macroscopic phase and the widely applied pseudo-phase model (PPM) is inaccurate. In the present work it was demonstrated that the aggregation number of micelles was constant at low concentrations enabling determination of the thermodynamic potentials by the MAM. A correlation between the aggregation number and the heat capacity was found, which implies that the dehydrated surface area of bile salts increases with the aggregation number. This is in accordance with Tanford's principles of opposing forces where neighbouring molecules in the aggregate are better able to shield from the surrounding hydrophilic environment when the aggregation number increases. PMID:25978555

  10. Manufacturing and automation

    Directory of Open Access Journals (Sweden)

    Ernesto Córdoba Nieto

    2010-04-01

    Full Text Available The article presents concepts and definitions from different sources concerning automation. The work approaches automation by virtue of the author’s experience in manufacturing production; why and how automation prolects are embarked upon is considered. Technological reflection regarding the progressive advances or stages of automation in the production area is stressed. Coriat and Freyssenet’s thoughts about and approaches to the problem of automation and its current state are taken and examined, especially that referring to the problem’s relationship with reconciling the level of automation with the flexibility and productivity demanded by competitive, worldwide manufacturing.

  11. Rapid analysis of three β-agonist residues in food of animal origin by automated on-line solid-phase extraction coupled to liquid chromatography and tandem mass spectrometry.

    Science.gov (United States)

    Mi, Jiebo; Li, Shujing; Xu, Hong; Liang, Wei; Sun, Tao

    2014-09-01

    An automated online solid-phase extraction with liquid chromatography and tandem mass spectrometry method was developed and validated for the detection of clenbuterol, salbutamol, and ractopamine in food of animal origin. The samples from the food matrix were pretreated with an online solid-phase extraction cartridge by Oasis MCX for <5 min after acid hydrolysis for 30 min. The peak focusing mode was used to elute the target compounds directly onto a C18 column. Chromatographic separation was achieved under gradient conditions using a mobile phase composed of acetonitrile/0.1% formic acid in aqueous solution. Each analyte was detected in two multiple reaction monitoring transitions via an electrospray ionization source in a positive mode. The relative standard deviations ranged from 2.6 to 10.5%, and recovery was between 76.7 and 107.2% at all quality control levels. The limits of quantification of three β-agonists were in the range of 0.024-0.29 μg/kg in pork, sausage, and milk powder, respectively. This newly developed method offers high sensitivity and minimum sample pretreatment for the high-throughput analysis of β-agonist residues. PMID:24916570

  12. Quantification of five compounds with heterogeneous physicochemical properties (morphine, 6-monoacetylmorphine, cyamemazine, meprobamate and caffeine) in 11 fluids and tissues, using automated solid-phase extraction and gas chromatography-tandem mass spectrometry.

    Science.gov (United States)

    Bévalot, Fabien; Bottinelli, Charline; Cartiser, Nathalie; Fanton, Laurent; Guitton, Jérôme

    2014-06-01

    An automated solid-phase extraction (SPE) protocol followed by gas chromatography coupled with tandem mass spectrometry was developed for quantification of caffeine, cyamemazine, meprobamate, morphine and 6-monoacetylmorphine (6-MAM) in 11 biological matrices [blood, urine, bile, vitreous humor, liver, kidney, lung and skeletal muscle, brain, adipose tissue and bone marrow (BM)]. The assay was validated for linearity, within- and between-day precision and accuracy, limits of quantification, selectivity, extraction recovery (ER), sample dilution and autosampler stability on BM. For the other matrices, partial validation was performed (limits of quantification, linearity, within-day precision, accuracy, selectivity and ER). The lower limits of quantification were 12.5 ng/mL(ng/g) for 6-MAM, morphine and cyamemazine, 100 ng/mL(ng/g) for meprobamate and 50 ng/mL(ng/g) for caffeine. Analysis of real-case samples demonstrated the performance of the assay in forensic toxicology to investigate challenging cases in which, for example, blood is not available or in which analysis in alternative matrices could be relevant. The SPE protocol was also assessed as an extraction procedure that could target other relevant analytes of interest. The extraction procedure was applied to 12 molecules of forensic interest with various physicochemical properties (alimemazine, alprazolam, amitriptyline, citalopram, cocaine, diazepam, levomepromazine, nordazepam, tramadol, venlafaxine, pentobarbital and phenobarbital). All drugs were able to be detected at therapeutic concentrations in blood and in the alternate matrices. PMID:24790060

  13. Assessment of the Molecular Expression and Structure of Gangliosides in Brain Metastasis of Lung Adenocarcinoma by an Advanced Approach Based on Fully Automated Chip-Nanoelectrospray Mass Spectrometry

    Science.gov (United States)

    Zamfir, Alina D.; Serb, Alina; Vukeli, Željka; Flangea, Corina; Schiopu, Catalin; Fabris, Dragana; Kalanj-Bognar, Svjetlana; Capitan, Florina; Sisu, Eugen

    2011-12-01

    Gangliosides (GGs), sialic acid-containing glycosphingolipids, are known to be involved in the invasive/metastatic behavior of brain tumor cells. Development of modern methods for determination of the variations in GG expression and structure during neoplastic cell transformation is a priority in the field of biomedical analysis. In this context, we report here on the first optimization and application of chip-based nanoelectrospray (NanoMate robot) mass spectrometry (MS) for the investigation of gangliosides in a secondary brain tumor. In our work a native GG mixture extracted and purified from brain metastasis of lung adenocarcinoma was screened by NanoMate robot coupled to a quadrupole time-of-flight MS. A native GG mixture from an age-matched healthy brain tissue, sampled and analyzed under identical conditions, served as a control. Comparative MS analysis demonstrated an evident dissimilarity in GG expression in the two tissue types. Brain metastasis is characterized by many species having a reduced N-acetylneuraminic acid (Neu5Ac) content, however, modified by fucosylation or O-acetylation such as Fuc-GM4, Fuc-GM3, di- O-Ac-GM1, O-Ac-GM3. In contrast, healthy brain tissue is dominated by longer structures exhibiting from mono- to hexasialylated sugar chains. Also, significant differences in ceramide composition were discovered. By tandem MS using collision-induced dissociation at low energies, brain metastasis-associated GD3 (d18:1/18:0) species as well as an uncommon Fuc-GM1 (d18:1/18:0) detected in the normal brain tissue could be structurally characterized. The novel protocol was able to provide a reliable compositional and structural characterization with high analysis pace and at a sensitivity situated in the fmol range.

  14. An automated swimming respirometer

    DEFF Research Database (Denmark)

    STEFFENSEN, JF; JOHANSEN, K; BUSHNELL, PG

    1984-01-01

    An automated respirometer is described that can be used for computerized respirometry of trout and sharks.......An automated respirometer is described that can be used for computerized respirometry of trout and sharks....

  15. Configuration Management Automation (CMA)

    Data.gov (United States)

    Department of Transportation — Configuration Management Automation (CMA) will provide an automated, integrated enterprise solution to support CM of FAA NAS and Non-NAS assets and investments. CMA...

  16. Students' Experiences with an Automated Essay Scorer

    Science.gov (United States)

    Scharber, Cassandra; Dexter, Sara; Riedel, Eric

    2008-01-01

    The purpose of this research is to analyze preservice teachers' use of and reactions to an automated essay scorer used within an online, case-based learning environment called ETIPS. Data analyzed include post-assignment surveys, a user log of students' actions within the cases, instructor-assigned scores on final essays, and interviews with four…

  17. Workflow automation architecture standard

    Energy Technology Data Exchange (ETDEWEB)

    Moshofsky, R.P.; Rohen, W.T. [Boeing Computer Services Co., Richland, WA (United States)

    1994-11-14

    This document presents an architectural standard for application of workflow automation technology. The standard includes a functional architecture, process for developing an automated workflow system for a work group, functional and collateral specifications for workflow automation, and results of a proof of concept prototype.

  18. A novel automated hydrophilic interaction liquid chromatography method using diode-array detector/electrospray ionization tandem mass spectrometry for analysis of sodium risedronate and related degradation products in pharmaceuticals.

    Science.gov (United States)

    Bertolini, Tiziana; Vicentini, Lorenza; Boschetti, Silvia; Andreatta, Paolo; Gatti, Rita

    2014-10-24

    A simple, sensitive and fast hydrophilic interaction liquid chromatography (HILIC) method using ultraviolet diode-array detector (UV-DAD)/electrospray ionization tandem mass spectrometry was developed for the automated high performance liquid chromatography (HPLC) determination of sodium risedronate (SR) and its degradation products in new pharmaceuticals. The chromatographic separations were performed on Ascentis Express HILIC 2.7μm (150mm×2.1mm, i.d.) stainless steel column (fused core). The mobile phase consisted of formate buffer solution (pH 3.4; 0.03M)/acetonitrile 42:58 and 45:55 (v/v) for granules for oral solution and effervescent tablet analysis, respectively, at a flow-rate of 0.2mL/min, setting the wavelength at 262nm. Stability characteristics of SR were evaluated by performing stress test studies. The main degradation product formed under oxidation conditions corresponding to sodium hydrogen (1-hydroxy-2-(1-oxidopyridin-3-yl)-1-phosphonoethyl)phosphonate was characterized by high performance liquid chromatography-electrospray ionization-mass tandem mass spectrometry (HPLC-ESI-MS/MS). The validation parameters such as linearity, sensitivity, accuracy, precision and selectivity were found to be highly satisfactory. Linear responses were observed in standard and in fortified placebo solutions. Intra-day precision (relative standard deviation, RSD) was ≤1.1% for peak area and ≤0.2% for retention times (tR) without significant differences between intra- and inter-day data. Recovery studies showed good results for all the examined compounds (from 98.7 to 101.0%) with RSD ranging from 0.6 to 0.7%. The limits of detection (LOD) and quantitation (LOQ) were 1 and 3ng/mL, respectively. The high stability of standard and sample solutions at room temperature means an undoubted advantage of the method allowing the simultaneous preparation of many samples and consecutive chromatographic analyses by using an autosampler. The developed stability indicating

  19. Shoe-String Automation

    Energy Technology Data Exchange (ETDEWEB)

    Duncan, M.L.

    2001-07-30

    Faced with a downsizing organization, serious budget reductions and retirement of key metrology personnel, maintaining capabilities to provide necessary services to our customers was becoming increasingly difficult. It appeared that the only solution was to automate some of our more personnel-intensive processes; however, it was crucial that the most personnel-intensive candidate process be automated, at the lowest price possible and with the lowest risk of failure. This discussion relates factors in the selection of the Standard Leak Calibration System for automation, the methods of automation used to provide the lowest-cost solution and the benefits realized as a result of the automation.

  20. Parameters Investigation of Mathematical Model of Productivity for Automated Line with Availability by DMAIC Methodology

    Directory of Open Access Journals (Sweden)

    Tan Chan Sin

    2014-01-01

    Full Text Available Automated line is widely applied in industry especially for mass production with less variety product. Productivity is one of the important criteria in automated line as well as industry which directly present the outputs and profits. Forecast of productivity in industry accurately in order to achieve the customer demand and the forecast result is calculated by using mathematical model. Mathematical model of productivity with availability for automated line has been introduced to express the productivity in terms of single level of reliability for stations and mechanisms. Since this mathematical model of productivity with availability cannot achieve close enough productivity compared to actual one due to lack of parameters consideration, the enhancement of mathematical model is required to consider and add the loss parameters that is not considered in current model. This paper presents the investigation parameters of productivity losses investigated by using DMAIC (Define, Measure, Analyze, Improve, and Control concept and PACE Prioritization Matrix (Priority, Action, Consider, and Eliminate. The investigated parameters are important for further improvement of mathematical model of productivity with availability to develop robust mathematical model of productivity in automated line.

  1. The Past, Present and Future of Supply-Chain Automation

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    In this article, we trace 20th-Century developments in the area of supply-chain automation. During the mass-production era, hard automation brought the automobile within the reach of the common man. Currently, the trend is to automate intercompany and company-to-customer relationships, also called collaboration, using the Internet. In between, several revolutions have occurred that were mainly sponsored by developments in computer and communications

  2. Automated interpretation of high-energy collision-induced dissociation spectra of singly protonated peptides by 'SeqMS', a software aid for de novo sequencing by tandem mass spectrometry.

    Science.gov (United States)

    Fernandez-de-Cossio, J; Gonzalez, J; Betancourt, L; Besada, V; Padron, G; Shimonishi, Y; Takao, T

    1998-01-01

    SeqMS, a software program designed for the automated interpretation of high-energy collision-induced dissociation (CID) mass spectra of singly protonated peptides ionized by fast atom bombardment, has been developed. The software is capable of probing the sequence of an unknown peptide, and even of certain modified peptides. The program, compiled for WINDOWS95 or NT, also permits the retrieval of raw data and the reconstruction of the spectra on a user-friendly graphical interface with the aid of several tools for processing the spectra, which include setting multiple threshold levels and automatic peak detection. SeqMS is capable of generating candidate sequences, based on the detected peaks, and of displaying the resulting assignments for each candidate in a spectrum or in tabular form. The software has the following capabilities: 1) the ions derived from backbone and side-chain fragmentations, internal and immonium ions, and side-chain loss ions can be used for calculation; 2) 18O-labeling of a peptide at the C terminus, a methodology which was developed to differentiate N-terminal from C-terminal ions, is applicable as an optional setting; 3) modified amino acids and N- or C-terminal blocking groups are taken into account for calculation according to the user's setting in a library; 4) amino acid composition and partial or complete amino acid sequence of a peptide can be used as input for calculation; 5) the assignments of signal output in a spectrum can be graphically edited, and then re-calculated based on the edited peaks. The efficacy of the program is demonstrated by testing 74 high-energy CID spectra, obtained using a four-sector instrument, of synthetic, proteolytic, and biologically active peptides, some of which contain modified groups. PMID:9842738

  3. Rapid and automated analysis of aflatoxin M1 in milk and dairy products by online solid phase extraction coupled to ultra-high-pressure-liquid-chromatography tandem mass spectrometry.

    Science.gov (United States)

    Campone, Luca; Piccinelli, Anna Lisa; Celano, Rita; Pagano, Imma; Russo, Mariateresa; Rastrelli, Luca

    2016-01-01

    This study reports a fast and automated analytical procedure for the analysis of aflatoxin M1 (AFM1) in milk and dairy products. The method is based on the simultaneous protein precipitation and AFM1 extraction, by salt-induced liquid-liquid extraction (SI-LLE), followed by an online solid-phase extraction (online SPE) coupled to ultra-high-pressure-liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis to the automatic pre-concentration, clean up and sensitive and selective determination of AFM1. The main parameters affecting the extraction efficiency and accuracy of the analytical method were studied in detail. In the optimal conditions, acetonitrile and NaCl were used as extraction/denaturant solvent and salting-out agent in SI-LLE, respectively. After centrifugation, the organic phase (acetonitrile) was diluted with water (1:9 v/v) and purified (1mL) by online C18 cartridge coupled with an UHPLC column. Finally, selected reaction monitoring (SRM) acquisition mode was applied to the detection of AFM1. Validation studies were carried out on different dairy products (whole and skimmed cow milk, yogurt, goat milk, and powder infant formula), providing method quantification limits about 25 times lower than AFM1 maximum levels permitted by EU regulation 1881/2006 in milk and dairy products for direct human consumption. Recoveries (86-102%) and repeatability (RSDeffects were observed in the different milk and dairy products studied. The proposed method improves the performance of AFM1 analysis in milk samples as AFM1 determination is performed with a degree of accuracy higher than the conventional methods. Other advantages are the reduction of sample preparation procedure, time and cost of the analysis, enabling high sample throughput that meet the current concerns of food safety and the public health protection. PMID:26589945

  4. The design and use of a simple System Suitability Test Mix for generic reverse phase high performance liquid chromatography-mass spectrometry systems and the implications for automated system monitoring using global software tracking.

    Science.gov (United States)

    Mutton, Ian; Boughtflower, Bob; Taylor, Nick; Brooke, Daniel

    2011-06-10

    The development of a seven-component test mixture designed for use with a generic gradient and a reversed-phase high performance liquid chromatography-mass spectrometry (RP-HPLC-MS) system is discussed. Unlike many test mixtures formulated in order to characterise column quality at neutral pH, the test mixture reported here was designed to permit an overall suitability assessment of the whole liquid chromatography-mass spectrometry (LCMS) system. The mixture is designed to test the chromatographic performance of the column as well as certain aspects of the performance of the individual instrumental components of the system. The System Suitability Test Mix can be used for low and high pH generic reverse phase LCMS analysis. Four phthalates are used: diethyl phthalate (DEP), diamyl phthalate (DAP), di-n-hexyl phthalate (DHP) and dioctyl phthalate (DOP). Three other probes are employed: 8-bromoguanosine (8-BG), amitryptyline (Ami), and 4-chlorocinnamic acid (4-CCA). We show that analysis of this test mixture can alert the user when any part of the system (instrument or column) contributes to loss of overall performance and may require remedial action and demonstrate that it can provide information that enables us to document data quality control. PMID:21543072

  5. Determination of the oxidative stress biomarker urinary 8-hydroxy-2'-deoxyguanosine by automated on-line in-tube solid-phase microextraction coupled with liquid chromatography-tandem mass spectrometry.

    Science.gov (United States)

    Kataoka, Hiroyuki; Mizuno, Keisuke; Oda, Eri; Saito, Akihiro

    2016-04-15

    A simple and sensitive method for the determination of 8-hydroxy-2'-deoxyguanosine (8-OHdG), a marker of oxidative DNA damage in human urine, was developed using automated on-line in-tube solid-phase microextraction (SPME) coupled with stable isotope-dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS). Creatinine was also analyzed simultaneously to normalize urine volume by the in-tube SPME LC-MS/MS method, and 8-OHdG and creatinine were separated within 3min using a Zorbax Eclipse XDB-C8 column. Electrospray MS/MS for these compounds was performed on an API 4000 triple quadruple mass spectrometer in the positive ion mode by multiple reaction monitoring. The optimum in-tube SPME conditions were 20 draw/eject cycles of 40μL of sample at a flow rate of 200μL/min using a Carboxen 1006 PLOT capillary column as an extraction device. The extracted compounds were easily desorbed from the capillary by passage of the mobile phase, and no carryover was observed. The calibration curve for 8-OHdG using its stable isotope-labeled internal standard was linear in the range of 0.05-10ng/mL, and the detection limit was 8.3pg/mL. The intra-day and inter-day precision (relative standard deviations) were below 3.1% and 9.6% (n=5), respectively. This method was applied successfully to the analysis of urine samples without any other pretreatment and interference peaks, with good recovery rates above 91% in spiked urine samples. The limits of quantification of 8-OHdG and creatinine in 0.1mL urine samples were about 0.32 and 0.69ng/mL (S/N=10), respectively. This method was utilized to assess the effects of smoking, green tea drinking and alcohol drinking on the urinary excretion of 8-OHdG. PMID:26349944

  6. Power, speed & automation with Adobe Photoshop

    CERN Document Server

    Scott, Geoff

    2012-01-01

    This is a must for the serious Photoshop user! Power, Speed & Automation explores how to customize and automate Photoshop to increase your speed and productivity.  With numerous step-by-step instructions, the authors-two of Adobe's own software developers!- walk you through the steps to best tailor Photoshop's interface to your personal workflow; write and apply Actions; and use batching and scripts to process large numbers of images quickly and automatically.  You will learn how to build your own dialogs and panels to improve your production workflows in Photoshop, the secrets of changing

  7. Automated stopcock actuator

    OpenAIRE

    Vandehey, N. T.; O'Neil, J.P.

    2015-01-01

    Introduction We have developed a low-cost stopcock valve actuator for radiochemistry automation built using a stepper motor and an Arduino, an open-source single-board microcontroller. The con-troller hardware can be programmed to run by serial communication or via two 5–24 V digital lines for simple integration into any automation control system. This valve actuator allows for automated use of a single, disposable stopcock, providing a number of advantages over stopcock manifold systems ...

  8. The Adaptive Automation Design

    OpenAIRE

    Calefato, Caterina; Montanari, Roberto; TESAURI, Francesco

    2008-01-01

    After considering the positive effects of adaptive automation implementation, this chapter focuses on two partly overlapping phenomena: on the one hand, the role of trust in automation is considered, particularly as to the effects of overtrust and mistrust in automation's reliability; on the other hand, long-term lack of exercise on specific operation may lead users to skill deterioration. As a future work, it will be interesting and challenging to explore the conjunction of adaptive automati...

  9. Service functional test automation

    OpenAIRE

    Hillah, Lom Messan; Maesano, Ariele-Paolo; Rosa, Fabio; Maesano, Libero; Lettere, Marco; Fontanelli, Riccardo

    2015-01-01

    This paper presents the automation of the functional test of services (black-box testing) and services architectures (grey-box testing) that has been developed by the MIDAS project and is accessible on the MIDAS SaaS. In particular, the paper illustrates the solutions of tough functional test automation problems such as: (i) the configuration of the automated test execution system against large and complex services architectures, (ii) the constraint-based test input generation, (iii) the spec...

  10. Automated Weather Observing System

    Data.gov (United States)

    Department of Transportation — The Automated Weather Observing System (AWOS) is a suite of sensors, which measure, collect, and disseminate weather data to help meteorologists, pilots, and flight...

  11. Laboratory Automation and Middleware.

    Science.gov (United States)

    Riben, Michael

    2015-06-01

    The practice of surgical pathology is under constant pressure to deliver the highest quality of service, reduce errors, increase throughput, and decrease turnaround time while at the same time dealing with an aging workforce, increasing financial constraints, and economic uncertainty. Although not able to implement total laboratory automation, great progress continues to be made in workstation automation in all areas of the pathology laboratory. This report highlights the benefits and challenges of pathology automation, reviews middleware and its use to facilitate automation, and reviews the progress so far in the anatomic pathology laboratory. PMID:26065792

  12. Automated cloning methods.; TOPICAL

    International Nuclear Information System (INIS)

    Argonne has developed a series of automated protocols to generate bacterial expression clones by using a robotic system designed to be used in procedures associated with molecular biology. The system provides plate storage, temperature control from 4 to 37 C at various locations, and Biomek and Multimek pipetting stations. The automated system consists of a robot that transports sources from the active station on the automation system. Protocols for the automated generation of bacterial expression clones can be grouped into three categories (Figure 1). Fragment generation protocols are initiated on day one of the expression cloning procedure and encompass those protocols involved in generating purified coding region (PCR)

  13. Automation of loading process equipment industrial companies

    Directory of Open Access Journals (Sweden)

    П.М. Павленко

    2006-01-01

    Full Text Available  The results of mathematical modeling the process of loading of the metalcutting equipment by theory methods of mass service are submitted. The results are used for construction of the appropriate program module of the automated system technological preparation of manufacture.

  14. Non-target screening of organic pollutants in sediments and sludges using gas chromatography-mass spectrometry and automated mass spectral deconvolution%气相色谱-质谱-自动质谱解卷积技术在沉积物和污泥中有机污染物非靶标筛查中的应用

    Institute of Scientific and Technical Information of China (English)

    王刚; 马慧莲; 王龙星; 陈吉平; 侯晓虹

    2015-01-01

    A screening method in the combination of ultrasonic extraction,gas chromatogra-phy-mass spectrometry detection and automated mass spectrometry deconvolution technique was developed for non-target screening of non-polar and weak polar pollutants in sediments and sludges. The samples were extracted by ultrasonication for 20 min using dichloromethane for three times. The extraction solutions were cleaned-up by gel permeation chromatography and a silica gel column,and then 3 g of copper powder was used to remove the sulfur by ultrasonica-tion for 10 min. Parallel experiments were carried out for 5 times and the RSDs were ranged from 5. 8% to 14. 9%. Automated mass spectral deconvolution &identification system( AMDIS) would improve the resolution of overlapping peaks,and identify the pure mass spectrum of the analytes in the cases of stronger background interference and co-extracted substances covering. Standard spectrum databases,such as NISTDRUG,NISTEPA,NISTFDA,Mass Spectral Library, etc,would qualitatively identify the organic pollutants in the samples. As a result,a total of 290 organic pollutants were identified,of which 190 and 153 pollutants were found in sediments and sludges,respectively. The identified pollutants included the Environmental Protection Agency (EPA)priority pollutants,pharmaceuticals,herbicides,antioxidants,intermediates,organic solvents and chemical raw materials. The proposed method is proved to be a promising one for non-target screening of complex matrix samples with the advantages of higher sensitivity and better repeatability.%以沉积物和污泥中非极性和弱极性有机污染物的非靶标筛查为目的,建立了一种超声波辅助提取-气相色谱-质谱结合自动解卷积技术的筛查方法。以二氯甲烷为溶剂超声波辅助提取样品3次,每次20 min,提取液经凝胶渗透色谱( GPC)和硅胶层析柱净化,再用3 g铜粉超声10 min除硫。前处理方法的重复性( RSD,n=5)为5.8

  15. 气相色谱-质谱-自动质谱解卷积技术在沉积物和污泥中有机污染物非靶标筛查中的应用%Non-target screening of organic pollutants in sediments and sludges using gas chromatography-mass spectrometry and automated mass spectral deconvolution

    Institute of Scientific and Technical Information of China (English)

    王刚; 马慧莲; 王龙星; 陈吉平; 侯晓虹

    2015-01-01

    A screening method in the combination of ultrasonic extraction,gas chromatogra-phy-mass spectrometry detection and automated mass spectrometry deconvolution technique was developed for non-target screening of non-polar and weak polar pollutants in sediments and sludges. The samples were extracted by ultrasonication for 20 min using dichloromethane for three times. The extraction solutions were cleaned-up by gel permeation chromatography and a silica gel column,and then 3 g of copper powder was used to remove the sulfur by ultrasonica-tion for 10 min. Parallel experiments were carried out for 5 times and the RSDs were ranged from 5. 8% to 14. 9%. Automated mass spectral deconvolution &identification system( AMDIS) would improve the resolution of overlapping peaks,and identify the pure mass spectrum of the analytes in the cases of stronger background interference and co-extracted substances covering. Standard spectrum databases,such as NISTDRUG,NISTEPA,NISTFDA,Mass Spectral Library, etc,would qualitatively identify the organic pollutants in the samples. As a result,a total of 290 organic pollutants were identified,of which 190 and 153 pollutants were found in sediments and sludges,respectively. The identified pollutants included the Environmental Protection Agency (EPA)priority pollutants,pharmaceuticals,herbicides,antioxidants,intermediates,organic solvents and chemical raw materials. The proposed method is proved to be a promising one for non-target screening of complex matrix samples with the advantages of higher sensitivity and better repeatability.%以沉积物和污泥中非极性和弱极性有机污染物的非靶标筛查为目的,建立了一种超声波辅助提取-气相色谱-质谱结合自动解卷积技术的筛查方法。以二氯甲烷为溶剂超声波辅助提取样品3次,每次20 min,提取液经凝胶渗透色谱( GPC)和硅胶层析柱净化,再用3 g铜粉超声10 min除硫。前处理方法的重复性( RSD,n=5)为5.8

  16. Zeta Spectral Action

    CERN Document Server

    Kurkov, Maxim A; Sakellariadou, Mairi; Watcharangkool, Apimook

    2014-01-01

    In this paper we propose a novel definition of the bosonic spectral action using zeta function regularization, in order to address the issues of renormalizability, ultraviolet completeness and spectral dimensions. We compare the zeta spectral action with the usual (cutoff based) spectral action and discuss its purely spectral origin, predictive power, stressing the importance of the issue of the three dimensionful fundamental constants, namely the cosmological constant, the Higgs vacuum expectation value, and the gravitational constant. We emphasize the fundamental role of the neutrino Majorana mass term for the structure of the bosonic action.

  17. Library Automation Style Guide.

    Science.gov (United States)

    Gaylord Bros., Liverpool, NY.

    This library automation style guide lists specific terms and names often used in the library automation industry. The terms and/or acronyms are listed alphabetically and each is followed by a brief definition. The guide refers to the "Chicago Manual of Style" for general rules, and a notes section is included for the convenience of individual…

  18. Automation in Warehouse Development

    NARCIS (Netherlands)

    Hamberg, R.; Verriet, J.

    2012-01-01

    The warehouses of the future will come in a variety of forms, but with a few common ingredients. Firstly, human operational handling of items in warehouses is increasingly being replaced by automated item handling. Extended warehouse automation counteracts the scarcity of human operators and support

  19. Automate functional testing

    Directory of Open Access Journals (Sweden)

    Ramesh Kalindri

    2014-06-01

    Full Text Available Currently, software engineers are increasingly turning to the option of automating functional tests, but not always have successful in this endeavor. Reasons range from low planning until over cost in the process. Some principles that can guide teams in automating these tests are described in this article.

  20. Automation in Immunohematology

    Directory of Open Access Journals (Sweden)

    Meenu Bajpai

    2012-01-01

    Full Text Available There have been rapid technological advances in blood banking in South Asian region over the past decade with an increasing emphasis on quality and safety of blood products. The conventional test tube technique has given way to newer techniques such as column agglutination technique, solid phase red cell adherence assay, and erythrocyte-magnetized technique. These new technologies are adaptable to automation and major manufacturers in this field have come up with semi and fully automated equipments for immunohematology tests in the blood bank. Automation improves the objectivity and reproducibility of tests. It reduces human errors in patient identification and transcription errors. Documentation and traceability of tests, reagents and processes and archiving of results is another major advantage of automation. Shifting from manual methods to automation is a major undertaking for any transfusion service to provide quality patient care with lesser turnaround time for their ever increasing workload. This article discusses the various issues involved in the process.

  1. Automated model building

    CERN Document Server

    Caferra, Ricardo; Peltier, Nicholas

    2004-01-01

    This is the first book on automated model building, a discipline of automated deduction that is of growing importance Although models and their construction are important per se, automated model building has appeared as a natural enrichment of automated deduction, especially in the attempt to capture the human way of reasoning The book provides an historical overview of the field of automated deduction, and presents the foundations of different existing approaches to model construction, in particular those developed by the authors Finite and infinite model building techniques are presented The main emphasis is on calculi-based methods, and relevant practical results are provided The book is of interest to researchers and graduate students in computer science, computational logic and artificial intelligence It can also be used as a textbook in advanced undergraduate courses

  2. Automation in Warehouse Development

    CERN Document Server

    Verriet, Jacques

    2012-01-01

    The warehouses of the future will come in a variety of forms, but with a few common ingredients. Firstly, human operational handling of items in warehouses is increasingly being replaced by automated item handling. Extended warehouse automation counteracts the scarcity of human operators and supports the quality of picking processes. Secondly, the development of models to simulate and analyse warehouse designs and their components facilitates the challenging task of developing warehouses that take into account each customer’s individual requirements and logistic processes. Automation in Warehouse Development addresses both types of automation from the innovative perspective of applied science. In particular, it describes the outcomes of the Falcon project, a joint endeavour by a consortium of industrial and academic partners. The results include a model-based approach to automate warehouse control design, analysis models for warehouse design, concepts for robotic item handling and computer vision, and auton...

  3. Advances in inspection automation

    Science.gov (United States)

    Weber, Walter H.; Mair, H. Douglas; Jansen, Dion; Lombardi, Luciano

    2013-01-01

    This new session at QNDE reflects the growing interest in inspection automation. Our paper describes a newly developed platform that makes the complex NDE automation possible without the need for software programmers. Inspection tasks that are tedious, error-prone or impossible for humans to perform can now be automated using a form of drag and drop visual scripting. Our work attempts to rectify the problem that NDE is not keeping pace with the rest of factory automation. Outside of NDE, robots routinely and autonomously machine parts, assemble components, weld structures and report progress to corporate databases. By contrast, components arriving in the NDT department typically require manual part handling, calibrations and analysis. The automation examples in this paper cover the development of robotic thickness gauging and the use of adaptive contour following on the NRU reactor inspection at Chalk River.

  4. Robust localisation of automated guided vehicles for computer-integrated manufacturing environments

    OpenAIRE

    Dixon, R. C.; Bright, G.; Harley, R.

    2013-01-01

    As industry moves toward an era of complete automation and mass customisation, automated guided vehicles (AGVs) are used as material handling systems. However, the current techniques that provide navigation, control, and manoeuvrability of automated guided vehicles threaten to create bottlenecks and inefficiencies in manufacturing environments that strive towards the optimisation of part production. This paper proposes a decentralised localisation technique for an automated guided vehicle ...

  5. Automated remedial assessment methodology software system

    Energy Technology Data Exchange (ETDEWEB)

    Whiting, M.; Wilkins, M.; Stiles, D.

    1994-11-01

    The Automated Remedial Analysis Methodology (ARAM) software system has been developed by the Pacific Northwest Laboratory to assist the U.S. Department of Energy (DOE) in evaluating cleanup options for over 10,000 contaminated sites across the DOE complex. The automated methodology comprises modules for decision logic diagrams, technology applicability and effectiveness rules, mass balance equations, cost and labor estimating factors and equations, and contaminant stream routing. ARAM is used to select technologies for meeting cleanup targets; determine the effectiveness of the technologies in destroying, removing, or immobilizing contaminants; decide the nature and amount of secondary waste requiring further treatment; and estimate the cost and labor involved when applying technologies.

  6. Automated remedial assessment methodology software system

    International Nuclear Information System (INIS)

    The Automated Remedial Analysis Methodology (ARAM) software system has been developed by the Pacific Northwest Laboratory to assist the U.S. Department of Energy (DOE) in evaluating cleanup options for over 10,000 contaminated sites across the DOE complex. The automated methodology comprises modules for decision logic diagrams, technology applicability and effectiveness rules, mass balance equations, cost and labor estimating factors and equations, and contaminant stream routing. ARAM is used to select technologies for meeting cleanup targets; determine the effectiveness of the technologies in destroying, removing, or immobilizing contaminants; decide the nature and amount of secondary waste requiring further treatment; and estimate the cost and labor involved when applying technologies

  7. Repeatability of Nerve conduction Measurements using Automation

    OpenAIRE

    Kong, Xuan; Lesser, Eugene A; Megerian, J. Thomas; Gozani, Shai N

    2006-01-01

    ABSTRACT Objective To quantify nerve conduction study (NCS) reproducibility utilizing an automated NCS system (NC-stat®, NeuroMetrix, Inc.). Method Healthy volunteers without neuropathic symptoms participated in the study. Their median, ulnar, peroneal, and tibial nerves were tested twice (7 days apart) by the same technician with an NC-stat® instrument. Pre-fabricated electrode arrays specific to each nerve were used. Both motor responses (compound motor action potential [CMAP] and F-waves –...

  8. Digital computers in action

    CERN Document Server

    Booth, A D

    1965-01-01

    Digital Computers in Action is an introduction to the basics of digital computers as well as their programming and various applications in fields such as mathematics, science, engineering, economics, medicine, and law. Other topics include engineering automation, process control, special purpose games-playing devices, machine translation and mechanized linguistics, and information retrieval. This book consists of 14 chapters and begins by discussing the history of computers, from the idea of performing complex arithmetical calculations to the emergence of a modern view of the structure of a ge

  9. Improvement of Test Automation

    OpenAIRE

    Räsänen, Timo

    2013-01-01

    The purpose for this study was to find out how to ensure that the automated testing of MME in the Network Verification will continue smooth and reliable while using the in-house developed test automation framework. The goal of this thesis was to reveal the reasons of the currently challenging situation and to find the key elements to be improved in the MME testing carried by the test automation. Also a reason for the study was to get solutions as to how to change the current procedures and wa...

  10. Chef infrastructure automation cookbook

    CERN Document Server

    Marschall, Matthias

    2013-01-01

    Chef Infrastructure Automation Cookbook contains practical recipes on everything you will need to automate your infrastructure using Chef. The book is packed with illustrated code examples to automate your server and cloud infrastructure.The book first shows you the simplest way to achieve a certain task. Then it explains every step in detail, so that you can build your knowledge about how things work. Eventually, the book shows you additional things to consider for each approach. That way, you can learn step-by-step and build profound knowledge on how to go about your configuration management

  11. Intentional Action and Action Slips.

    Science.gov (United States)

    Heckhausen, Heinz; Beckmann, Jurgen

    1990-01-01

    An explanation of action slips is offered that examines controlled actions in the context of an intentional behavior theory. Actions are considered guided by mentally represented intentions, subdivided into goal intentions and contingent instrumental intentions. Action slips are categorized according to problem areas in the enactment of goal…

  12. Automated Vehicles Symposium 2014

    CERN Document Server

    Beiker, Sven; Road Vehicle Automation 2

    2015-01-01

    This paper collection is the second volume of the LNMOB series on Road Vehicle Automation. The book contains a comprehensive review of current technical, socio-economic, and legal perspectives written by experts coming from public authorities, companies and universities in the U.S., Europe and Japan. It originates from the Automated Vehicle Symposium 2014, which was jointly organized by the Association for Unmanned Vehicle Systems International (AUVSI) and the Transportation Research Board (TRB) in Burlingame, CA, in July 2014. The contributions discuss the challenges arising from the integration of highly automated and self-driving vehicles into the transportation system, with a focus on human factors and different deployment scenarios. This book is an indispensable source of information for academic researchers, industrial engineers, and policy makers interested in the topic of road vehicle automation.

  13. I-94 Automation FAQs

    Data.gov (United States)

    Department of Homeland Security — In order to increase efficiency, reduce operating costs and streamline the admissions process, U.S. Customs and Border Protection has automated Form I-94 at air and...

  14. Automated Vehicles Symposium 2015

    CERN Document Server

    Beiker, Sven

    2016-01-01

    This edited book comprises papers about the impacts, benefits and challenges of connected and automated cars. It is the third volume of the LNMOB series dealing with Road Vehicle Automation. The book comprises contributions from researchers, industry practitioners and policy makers, covering perspectives from the U.S., Europe and Japan. It is based on the Automated Vehicles Symposium 2015 which was jointly organized by the Association of Unmanned Vehicle Systems International (AUVSI) and the Transportation Research Board (TRB) in Ann Arbor, Michigan, in July 2015. The topical spectrum includes, but is not limited to, public sector activities, human factors, ethical and business aspects, energy and technological perspectives, vehicle systems and transportation infrastructure. This book is an indispensable source of information for academic researchers, industrial engineers and policy makers interested in the topic of road vehicle automation.

  15. Hydrometeorological Automated Data System

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Office of Hydrologic Development of the National Weather Service operates HADS, the Hydrometeorological Automated Data System. This data set contains the last...

  16. An automated Certification Authority

    CERN Document Server

    Shamardin, L V

    2002-01-01

    This note describe an approach to building an automated Certification Authority. It is compatible with basic requirements of RFC2527. It also supports Registration Authorities and Globus Toolkit grid-cert-renew automatic certificate renewal.

  17. Disassembly automation automated systems with cognitive abilities

    CERN Document Server

    Vongbunyong, Supachai

    2015-01-01

    This book presents a number of aspects to be considered in the development of disassembly automation, including the mechanical system, vision system and intelligent planner. The implementation of cognitive robotics increases the flexibility and degree of autonomy of the disassembly system. Disassembly, as a step in the treatment of end-of-life products, can allow the recovery of embodied value left within disposed products, as well as the appropriate separation of potentially-hazardous components. In the end-of-life treatment industry, disassembly has largely been limited to manual labor, which is expensive in developed countries. Automation is one possible solution for economic feasibility. The target audience primarily comprises researchers and experts in the field, but the book may also be beneficial for graduate students.

  18. Automated security management

    CERN Document Server

    Al-Shaer, Ehab; Xie, Geoffrey

    2013-01-01

    In this contributed volume, leading international researchers explore configuration modeling and checking, vulnerability and risk assessment, configuration analysis, and diagnostics and discovery. The authors equip readers to understand automated security management systems and techniques that increase overall network assurability and usability. These constantly changing networks defend against cyber attacks by integrating hundreds of security devices such as firewalls, IPSec gateways, IDS/IPS, authentication servers, authorization/RBAC servers, and crypto systems. Automated Security Managemen

  19. Automating Supplier Selection Procedures

    OpenAIRE

    Davidrajuh, Reggie

    2001-01-01

    This dissertation describes a methodology, tools, and implementation techniques of automating supplier selection procedures of a small and medium-sized agile virtual enterprise. Firstly, a modeling approach is devised that can be used to model the supplier selection procedures of an enterprise. This modeling approach divides the supplier selection procedures broadly into three stages, the pre-selection, selection, and post-selection stages. Secondly, a methodology is presented for automating ...

  20. Taiwan Automated Telescope Network

    OpenAIRE

    Shuhrat Ehgamberdiev; Alexander Serebryanskiy; Antonio Jimenez; Li-Han Wang; Ming-Tsung Sun; Javier Fernandez Fernandez; Dean-Yi Chou

    2010-01-01

    A global network of small automated telescopes, the Taiwan Automated Telescope (TAT) network, dedicated to photometric measurements of stellar pulsations, is under construction. Two telescopes have been installed in Teide Observatory, Tenerife, Spain and Maidanak Observatory, Uzbekistan. The third telescope will be installed at Mauna Loa Observatory, Hawaii, USA. Each system uses a 9-cm Maksutov-type telescope. The effective focal length is 225 cm, corresponding to an f-ratio of 25. The field...

  1. Automated Lattice Perturbation Theory

    Energy Technology Data Exchange (ETDEWEB)

    Monahan, Christopher

    2014-11-01

    I review recent developments in automated lattice perturbation theory. Starting with an overview of lattice perturbation theory, I focus on the three automation packages currently "on the market": HiPPy/HPsrc, Pastor and PhySyCAl. I highlight some recent applications of these methods, particularly in B physics. In the final section I briefly discuss the related, but distinct, approach of numerical stochastic perturbation theory.

  2. Automated functional software testing

    OpenAIRE

    Jelnikar, Kristina

    2009-01-01

    The following work describes an approach to software test automation of functional testing. In the introductory part we are introducing what testing problems development companies are facing. The second chapter describes some testing methods, what role does testing have in software development, some approaches to software development and the meaning of testing environment. Chapter 3 is all about test automation. After a brief historical presentation, we are demonstrating through s...

  3. Instant Sikuli test automation

    CERN Document Server

    Lau, Ben

    2013-01-01

    Get to grips with a new technology, understand what it is and what it can do for you, and then get to work with the most important features and tasks. A concise guide written in an easy-to follow style using the Starter guide approach.This book is aimed at automation and testing professionals who want to use Sikuli to automate GUI. Some Python programming experience is assumed.

  4. Differential employment rates in the journalism and mass communication labor force based on gender, race and ethnicity: Exploring the impact of affirmative action

    NARCIS (Netherlands)

    L.B. Becker; E. Lauf; W. Lowrey

    1999-01-01

    This paper examines whether gender, race, and ethnicity are associated with employment in the journalism and mass communication labor market and—if discrepancies in employment exist—what explanations might he offered for them. The data show strong evidence that race and ethnicity are associated with

  5. Effects of Automation Types on Air Traffic Controller Situation Awareness and Performance

    Science.gov (United States)

    Sethumadhavan, A.

    2009-01-01

    The Joint Planning and Development Office has proposed the introduction of automated systems to help air traffic controllers handle the increasing volume of air traffic in the next two decades (JPDO, 2007). Because fully automated systems leave operators out of the decision-making loop (e.g., Billings, 1991), it is important to determine the right level and type of automation that will keep air traffic controllers in the loop. This study examined the differences in the situation awareness (SA) and collision detection performance of individuals when they worked with information acquisition, information analysis, decision and action selection and action implementation automation to control air traffic (Parasuraman, Sheridan, & Wickens, 2000). When the automation was unreliable, the time taken to detect an upcoming collision was significantly longer for all the automation types compared with the information acquisition automation. This poor performance following automation failure was mediated by SA, with lower SA yielding poor performance. Thus, the costs associated with automation failure are greater when automation is applied to higher order stages of information processing. Results have practical implications for automation design and development of SA training programs.

  6. Automated diagnosis of LC-MS/MS performance

    OpenAIRE

    Xu, Hua; Freitas, Michael A.

    2009-01-01

    Summary: We report a software scheme for automated diagnosis of liquid chromatography tandem mass spectrometry (LC-MS/MS) system performance. The proposed software scheme provides a robust framework for establishing automated diagnosis of LC-MS/MS system performance for a variety of instruments and experiments. This schematic consists of four main software components: (i) data conversion, (ii) peptide identification, (iii) LC retention time analysis and (iv) system performance evaluation. The...

  7. Automated Camera Calibration

    Science.gov (United States)

    Chen, Siqi; Cheng, Yang; Willson, Reg

    2006-01-01

    Automated Camera Calibration (ACAL) is a computer program that automates the generation of calibration data for camera models used in machine vision systems. Machine vision camera models describe the mapping between points in three-dimensional (3D) space in front of the camera and the corresponding points in two-dimensional (2D) space in the camera s image. Calibrating a camera model requires a set of calibration data containing known 3D-to-2D point correspondences for the given camera system. Generating calibration data typically involves taking images of a calibration target where the 3D locations of the target s fiducial marks are known, and then measuring the 2D locations of the fiducial marks in the images. ACAL automates the analysis of calibration target images and greatly speeds the overall calibration process.

  8. Automated telescope scheduling

    Science.gov (United States)

    Johnston, Mark D.

    1988-08-01

    With the ever increasing level of automation of astronomical telescopes the benefits and feasibility of automated planning and scheduling are becoming more apparent. Improved efficiency and increased overall telescope utilization are the most obvious goals. Automated scheduling at some level has been done for several satellite observatories, but the requirements on these systems were much less stringent than on modern ground or satellite observatories. The scheduling problem is particularly acute for Hubble Space Telescope: virtually all observations must be planned in excruciating detail weeks to months in advance. Space Telescope Science Institute has recently made significant progress on the scheduling problem by exploiting state-of-the-art artificial intelligence software technology. What is especially interesting is that this effort has already yielded software that is well suited to scheduling groundbased telescopes, including the problem of optimizing the coordinated scheduling of more than one telescope.

  9. Methodical Approaches to Creation of Dividing Automation at Industrial Enterprises with Generating Power Plants

    Directory of Open Access Journals (Sweden)

    E. V. Kalentionok

    2014-07-01

    Full Text Available The paper considers a problem pertaining to creation of dividing automation at industrial enterprises which have their own generating plants. Algorithms for action of dividing automation that permit to ensure minimum possible power non-balance while using generating plants for autonomous operation and possible parameters for its response are proposed in the paper.

  10. Myths in test automation

    OpenAIRE

    Jazmine Francis

    2015-01-01

    Myths in automation of software testing is an issue of discussion that echoes about the areas of service in validation of software industry. Probably, the first though that appears in knowledgeable reader would be Why this old topic again? What's New to discuss the matter? But, for the first time everyone agrees that undoubtedly automation testing today is not today what it used to be ten or fifteen years ago, because it has evolved in scope and magnitude. What began as a simple linear script...

  11. Automated phantom assay system

    International Nuclear Information System (INIS)

    This paper describes an automated phantom assay system developed for assaying phantoms spiked with minute quantities of radionuclides. The system includes a computer-controlled linear-translation table that positions the phantom at exact distances from a spectrometer. A multichannel analyzer (MCA) interfaces with a computer to collect gamma spectral data. Signals transmitted between the controller and MCA synchronize data collection and phantom positioning. Measured data are then stored on disk for subsequent analysis. The automated system allows continuous unattended operation and ensures reproducible results

  12. Determination and confirmation of parent and total ractopamine in bovine, swine, and turkey tissues by liquid chromatography with tandem mass spectrometry: First Action 2011.23.

    Science.gov (United States)

    Burnett, Thomas J; Rodewald, John M; Brunelle, Sharon L; Neeley, Mark; Wallace, Michael; Connolly, Paul; Coleman, Mark R

    2012-01-01

    A candidate method selected by the AOAC Expert Review Panel (ERP) for Ractopamine for determination and confirmation of parent and total ractopamine by LC/MS/MS was validated in a single laboratory for bovine, swine, and turkey tissues. The candidate method utilizes methanol extraction of the tissues, followed by an optional enzymatic hydrolysis for determination of total (parent plus conjugate) ractopamine. A mixed-mode cation exchange SPE cartridge is used to purify the initial extract before LC/MS/MS. Matrix-matched standards and a ractopamine-d6 internal standard are used for quantification of parent and total ractopamine in unknown samples. Validation data demonstrated that mean intertrial recoveries for ractopamine across all concentrations tested ranged from 79.7 to 102.2% for parent ractopamine and from 79.0 to 100.0% when a hydrolysis step was included. Intertrial repeatability precision ranged from 2.44 to 11.1% for parent ractopamine and 4.97 to 15.0% with hydrolysis. Estimated LOD values were below 0.1 ng/g and LOQ values were validated at 0.25x the maximum residue limits. The data satisfy the requirements of the AOAC Stakeholder Panel for Veterinary Drug Residue Methods for single laboratory validation studies. The method was awarded Official Methods of Analysis First Action 2011.23 by the AOAC ERP on Veterinary Drug Residues. PMID:23175955

  13. Working together on automated vehicle guidance AVG : preliminary business plan, abridged version.

    NARCIS (Netherlands)

    Awareness (ed.)

    1998-01-01

    This plan describes the questions which will have to be answered in the short term, and the action which need to be taken in a phased and structured manner to gain insight into the potential of automated vehicle guidance (AVG).

  14. Determination of narasin and monensin in bovine, swine, and chicken tissues by liquid chromatography with tandem mass spectrometry: first action 2011.24.

    Science.gov (United States)

    Burnett, Thomas J; Lombardi, Kim; Rodewald, John M; Brunelle, Sharon L; MacDougall, Johnnie; Coleman, Mark R

    2012-01-01

    The single-laboratory validation (SLV) of an LC-MS/MS method for determination and confirmation of two ionophores, narasin and monensin, in animal tissues is described. The data demonstrated linearity of matrix-matched calibration curves using a weighted (1/x) regression and selectivity of the method for narasin and monensin in the presence of lasalocid, salinomycin, maduramycin, nicarbazin, and sulfadiazine. Recoveries varied from 86.2 to 103.5% for narasin and 89.1 to 105.1% for monensin. Intertrial repeatability precision [relative standard deviation of repeatability (RSDr)] varied from 3.9 to 13.8% for narasin and 3.3 to 16.3% for monensin in fortified tissue. Precision of the method was verified in incurred tissues. The LOQ of the method was validated and ranged from 0.45 ng/g in milk, to 4.0 ng/g in chicken fat, but was 0.75 ng/g for most tissues. Two confirmatory ions for each analyte were examined across all matrixes, resulting in estimated false-negative rates of 0.00% (95% confidence interval of 0.00-0.68%) for monensin ions (540 samples) compared to the U.S. and European Union (EU) acceptance criteria. The confirmatory ions for narasin demonstrated 0.00% false-negative rates (95% confidence interval of 0.00-0.58%) when compared to either the U.S. or EU criteria in 630 samples. The method was robust when small changes in method parameters were made and stability of fortified tissues, extracts, and calibration solutions were estimated. The data satisfy the requirements of the AOAC Stakeholder Panel on Veterinary Drug Residue for SLV studies, and the method was adopted Official Methods of Analysis First Action 2011.24 by the AOAC Expert Review Panel on Veterinary Drug Residues. PMID:22970563

  15. Automated conflict resolution issues

    Science.gov (United States)

    Wike, Jeffrey S.

    1991-01-01

    A discussion is presented of how conflicts for Space Network resources should be resolved in the ATDRSS era. The following topics are presented: a description of how resource conflicts are currently resolved; a description of issues associated with automated conflict resolution; present conflict resolution strategies; and topics for further discussion.

  16. Protokoller til Home Automation

    DEFF Research Database (Denmark)

    Kjær, Kristian Ellebæk

    2008-01-01

    computer, der kan skifte mellem foruddefinerede indstillinger. Nogle gange kan computeren fjernstyres over internettet, så man kan se hjemmets status fra en computer eller måske endda fra en mobiltelefon. Mens nævnte anvendelser er klassiske indenfor home automation, er yderligere funktionalitet dukket op...

  17. Myths in test automation

    Directory of Open Access Journals (Sweden)

    Jazmine Francis

    2015-01-01

    Full Text Available Myths in automation of software testing is an issue of discussion that echoes about the areas of service in validation of software industry. Probably, the first though that appears in knowledgeable reader would be Why this old topic again? What's New to discuss the matter? But, for the first time everyone agrees that undoubtedly automation testing today is not today what it used to be ten or fifteen years ago, because it has evolved in scope and magnitude. What began as a simple linear scripts for web applications today has a complex architecture and a hybrid framework to facilitate the implementation of testing applications developed with various platforms and technologies. Undoubtedly automation has advanced, but so did the myths associated with it. The change in perspective and knowledge of people on automation has altered the terrain. This article reflects the points of views and experience of the author in what has to do with the transformation of the original myths in new versions, and how they are derived; also provides his thoughts on the new generation of myths.

  18. Automated data model evaluation

    International Nuclear Information System (INIS)

    Modeling process is essential phase within information systems development and implementation. This paper presents methods and techniques for analysis and evaluation of data model correctness. Recent methodologies and development results regarding automation of the process of model correctness analysis and relations with ontology tools has been presented. Key words: Database modeling, Data model correctness, Evaluation

  19. Automated solvent concentrator

    Science.gov (United States)

    Griffith, J. S.; Stuart, J. L.

    1976-01-01

    Designed for automated drug identification system (AUDRI), device increases concentration by 100. Sample is first filtered, removing particulate contaminants and reducing water content of sample. Sample is extracted from filtered residue by specific solvent. Concentrator provides input material to analysis subsystem.

  20. ELECTROPNEUMATIC AUTOMATION EDUCATIONAL LABORATORY

    OpenAIRE

    Dolgorukov, S. O.; National Aviation University; Roman, B. V.; National Aviation University

    2013-01-01

    The article reflects current situation in education regarding mechatronics learning difficulties. Com-plex of laboratory test benches on electropneumatic automation are considered as a tool in advancing through technical science. Course of laboratory works developed to meet the requirement of efficient and reliable way of practical skills acquisition is regarded the simplest way for students to learn the ba-sics of mechatronics.

  1. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (October - December 1993) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  2. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (July - September 1992) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  3. Automating spectral measurements

    Science.gov (United States)

    Goldstein, Fred T.

    2008-09-01

    This paper discusses the architecture of software utilized in spectroscopic measurements. As optical coatings become more sophisticated, there is mounting need to automate data acquisition (DAQ) from spectrophotometers. Such need is exacerbated when 100% inspection is required, ancillary devices are utilized, cost reduction is crucial, or security is vital. While instrument manufacturers normally provide point-and-click DAQ software, an application programming interface (API) may be missing. In such cases automation is impossible or expensive. An API is typically provided in libraries (*.dll, *.ocx) which may be embedded in user-developed applications. Users can thereby implement DAQ automation in several Windows languages. Another possibility, developed by FTG as an alternative to instrument manufacturers' software, is the ActiveX application (*.exe). ActiveX, a component of many Windows applications, provides means for programming and interoperability. This architecture permits a point-and-click program to act as automation client and server. Excel, for example, can control and be controlled by DAQ applications. Most importantly, ActiveX permits ancillary devices such as barcode readers and XY-stages to be easily and economically integrated into scanning procedures. Since an ActiveX application has its own user-interface, it can be independently tested. The ActiveX application then runs (visibly or invisibly) under DAQ software control. Automation capabilities are accessed via a built-in spectro-BASIC language with industry-standard (VBA-compatible) syntax. Supplementing ActiveX, spectro-BASIC also includes auxiliary serial port commands for interfacing programmable logic controllers (PLC). A typical application is automatic filter handling.

  4. Determination and confirmation of nicarbazin, measured as 4,4-dinitrocarbanilide (DNC), in chicken tissues by liquid chromatography with tandem mass spectrometry: First Action 2013.07.

    Science.gov (United States)

    Coleman, Mark R; Rodewald, John M; Brunelle, Sharon L; Nelson, Maria; Bailey, Lauryn; Burnett, Thomas J

    2014-01-01

    A single-laboratory validation (SLV) study was conducted on an LC/MS/MS method for the determination and confirmation of nicarbazin, expressed as 4,4-dinitrocarbanilide (DNC), in chicken tissues, including liver, kidney, muscle, skin with adhering fat, and eggs. Linearity was demonstrated with DNC standard curve solutions using a weighted (1/x) regression and confirmed with matrix-matched standards. Intertrial repeatability precision (relative standard deviation of repeatability; RSD(r) was from 2.5 to 11.3%, as determined in fortified tissues. The precision was verified with incurred tissue, and varied from 0.53 to 2.5%. Average recoveries ranged from 82% in egg to 98% in kidney. Although the average recoveries across all concentrations were within the acceptable range, the method was improved with the inclusion of an internal standard and the use of matrix-matched standards. Accuracy for the improved method in chicken liver varied from 93 to 99% across all concentrations (100-8000 ng/g) compared to recoveries below 80% at concentrations, between 100-400 ng/g in chicken liver for the original method. The limit of detection was estimated to be less than 3.0 ng/g in all tissue types, and the limit of quantitation was validated at 20 ng/g. Based on confirmatory ion ratios and peak retention times, the false-negative rate was estimated as 0.00% (95% confidence limits 0.00, 0.74%) from 484 fortified samples and 12 incurred residue samples analyzed using the U.S. and EU confirmation criteria. Small variations to the method parameters, with the exception of injection volume, did not have a significant effect on recoveries. Stability was determined for fortified tissues, extracts, and standard curve solutions. The data collected in this study satisfy the requirements of SLV studies established by the AOAC Stakeholder Panel for Veterinary Drug Residue and the method was awarded First Action Official Method status by the Expert Review Panel for Veterinary Drug Residues on

  5. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (January--March 1989) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. Also included are a number of enforcement actions that had been previously resolved but not published in this NUREG. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  6. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (January--March 1990) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. Also included are a number of enforcement actions that had been previously resolved but not published in this NUREG. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  7. The Automated Planet Finder's automation & first two years of science

    Science.gov (United States)

    Burt, Jennifer; Laughlin, Greg; Vogt, Steven S.; Holden, Bradford

    2016-01-01

    The Automated Planet Finder (APF) is the newest facility at Lick Observatory, comprised of a 2.4m telescope coupled with the high-resolution Levy echelle spectrograph. Purpose built for exoplanet detection and characterization, 80% of the telescope's observing time is dedicated to these science goals. The APF has demonstrated 1 m/s radial velocity precision on bright, RV standard stars and performs with the same speed-on-sky as Keck/HIRES when observing M-dwarfs.The telesope is fully automated for RV operations, using a dynamic scheduler that makes informed decisions on which targets to observe based on scientific interest, desired cadence, required precision levels and current observing conditions, all on a minute-to-minute basis. This ensures that time is not wasted chasing non-optimal targets on nights with poor conditions and enables rapid changes to the overall science observing strategy.The APF has contributed to the detection of four planetary systems in its first two years of scientific operations. Our most recent detection is that of a 6-planet system around the bright (V=5.5), nearby (d=6.5pc), K3V star HD 219134. The planets in this system have masses ranging from 3.5 to108 MEarth, with orbital periods from 3 to 2247 days. An independent detection of the inner 4 planets in this system by the HARPS-N team has shown that the 3d planet transits the star, making this system ideal for follow-up observations.I will discuss the design and implementation of the APF's dynamic scheduler, the telescope's planet detections to date, overall performance results of the telescope and our future observing strategy.

  8. Levels of automation and user control - evaluation of a turbine automation interface

    Energy Technology Data Exchange (ETDEWEB)

    Andersson, Jonas (Chalmers Univ. of Technology (Sweden))

    2008-10-15

    The study was performed during the annual operator training at the Studsvik nuclear power plant simulator facility in Nykoeping, Sweden. The participating operators came from the Oskarshamn 3 nuclear power plant. In the study, seven nuclear power plant turbine operators were interviewed concerning their use of the automatic turbine system. A field study approach together with a heuristic usability evaluation was made to assess how the operators are affected by use of automation in the control room setting. The purpose of the study was to examine how operator performance is affected by varying levels of automation in nuclear power plant turbine operation. The Automatic Turbine System (ATS) was evaluated to clarify how the ATS interface design supports the operators work. The results show that during manual control the operators experience loss of speed and accuracy in performing actions together with difficulty of dividing attention between performing a task and overall monitoring, as the major problems. The positive aspects of manual operations lie in increased feeling of being in control when performing actions by hand. With higher levels of automation the problems shift to issues concerning difficulty of following the automatic sequences and loosing track in procedures. As the level of automation gets higher, the need of feedback increases which means that information presentation also becomes more important. The use of the semiautomatic, step-mode is often preferred by the operators since it combines the speed and accuracy of the automation with the ability of maintaining the feeling of being in control. Further, a number of usability related concerns was found in the ATS interface. The operators especially experience the presentation of the conditions that manage the automatic sequences as difficult to perceive. (author)

  9. Levels of automation and user control - evaluation of a turbine automation interface

    International Nuclear Information System (INIS)

    The study was performed during the annual operator training at the Studsvik nuclear power plant simulator facility in Nykoeping, Sweden. The participating operators came from the Oskarshamn 3 nuclear power plant. In the study, seven nuclear power plant turbine operators were interviewed concerning their use of the automatic turbine system. A field study approach together with a heuristic usability evaluation was made to assess how the operators are affected by use of automation in the control room setting. The purpose of the study was to examine how operator performance is affected by varying levels of automation in nuclear power plant turbine operation. The Automatic Turbine System (ATS) was evaluated to clarify how the ATS interface design supports the operators work. The results show that during manual control the operators experience loss of speed and accuracy in performing actions together with difficulty of dividing attention between performing a task and overall monitoring, as the major problems. The positive aspects of manual operations lie in increased feeling of being in control when performing actions by hand. With higher levels of automation the problems shift to issues concerning difficulty of following the automatic sequences and loosing track in procedures. As the level of automation gets higher, the need of feedback increases which means that information presentation also becomes more important. The use of the semiautomatic, step-mode is often preferred by the operators since it combines the speed and accuracy of the automation with the ability of maintaining the feeling of being in control. Further, a number of usability related concerns was found in the ATS interface. The operators especially experience the presentation of the conditions that manage the automatic sequences as difficult to perceive. (au)

  10. Single-run determination of polybrominated diphenyl ethers (PBDEs) di- to deca-brominated in fish meal, fish oil and fish feed by isotope dilution: Application of automated sample purification and gas chromatography/ion trap tandem mass spectrometry (GC/ITMS)

    International Nuclear Information System (INIS)

    The present paper describes the application of automated cleanup and fractionation procedures of the Power Prep system (Fluid Management Systems) for the determination of polybrominated diphenyl ethers (PBDEs) in feeding stuffs and fish meal and oil. Gas chromatography (GC) separation followed by ion trap tandem mass spectrometry detection in EI mode (ITMS) allowed the analysis of di- to deca-BDEs in the samples matrices used in fish aquaculture. The method developed enabled the determination of 26 native PBDE congeners and 11 13C12-labelled congeners, including deca-BDE 209, in a single-run analysis, using isotope dilution. The automated cleanup, consisting of a succession of multilayer silica and basic alumina columns previously applied by Wyrzykowska et al. (2009) in combustion flue gas, was succesfully applied in our complex matrices. The method allowed an increase in productivity, i.e. lower time was required to process samples, and simultaneous purification of several samples was achieved at a time, reducing analyst dedication and human error input. Average recoveries of 43-96% were obtained. GC/ITMS can overcome the complexity originating from the sample matrix, eliminating matrix effects by tandem MS, to enable the detection of congeners penta- to nona-BDEs where interferent masses were present. The provisional detection limits, estimated in the samples, were 5-30 pg for di-, tri-, tetra-, and penta-BDEs, 20-65 pg for hexa-, hepta-, octa- and nona-BDEs, and 105 pg for deca-BDE. Reduction of deca-BDE 209 blank values is of concern to ongoing research. Good accuracy was obtained by application of the whole procedure, representing an efficient, low-cost and fast alternative for routine analyses.

  11. Automation at NRCN Dosimetry Laboratory

    International Nuclear Information System (INIS)

    Running a dosimetric service based on TLD technology such as at the Nuclear Research Centre Negev (NRCN) requires a large group of workers to carry out simple mechanical actions such as opening and closing TLD badges, placing and removal of TLD cards from the badges and operating the TLD reader. These actions can be automated to free human resources for other assignments and to improve the quality assurance. At NRCN a project was undertaken to design and build a robotic system based on a manipulator arm. The design was based on the experience achieved with an earlier prototype (1,2). The system stores the TLD badges in special designed boxes, which are transported and stored in computer defined bins. The robotic arm loads and unloads TLD cards to the badges, and loads/unloads the cards to a magazine for the TLD reader. At the Nuclear Research Center Negev (NRCN) each badge is assigned to a specific worker and bears a sticker containing the worker's personal details, also in a machine readable form (barcode). In order to establish a proper QA check, a barcode reader records the information on the badge and on the TLD card placed in this badge and checks their compatibility with the information contained in the main database. Besides the TLD cards loading/unloading station, there is a contamination check station, a cards cleaning station and a UV irradiation box used to reduce the history dependent residual dose. The system was installed at the NRCN dosimetry laboratory It was successfully tested for several hundreds of cycles and will become operational in the first quarter of 2014. As far as we know, there is no similar product available for automatic handling in a TLD laboratory

  12. Automated Preferences Elicitation

    Czech Academy of Sciences Publication Activity Database

    Kárný, Miroslav; Guy, Tatiana Valentine

    Prague : Institute of Information Theory and Automation, 2011, s. 20-25. ISBN 978-80-903834-6-3. [The 2nd International Workshop od Decision Making with Multiple Imperfect Decision Makers. Held in Conjunction with the 25th Annual Conference on Neural Information Processing Systems (NIPS 2011). Sierra Nevada (ES), 16.12.2011-16.12.2011] R&D Projects: GA MŠk 1M0572; GA ČR GA102/08/0567 Institutional research plan: CEZ:AV0Z10750506 Keywords : elicitation * decision making * Bayesian decision making * fully probabilistic design Subject RIV: BB - Applied Statistics, Operational Research http://library.utia.cas.cz/separaty/2011/AS/karny-automated preferences elicitation.pdf

  13. Automated drawing generation system

    International Nuclear Information System (INIS)

    Since automated CAD drawing generation systems still require human intervention, improvements were focussed on an interactive processing section (data input and correcting operation) which necessitates a vast amount of work. As a result, human intervention was eliminated, the original objective of a computerized system. This is the first step taken towards complete automation. The effects of development and commercialization of the system are as described below. (1) The interactive processing time required for generating drawings was improved. It was determined that introduction of the CAD system has reduced the time required for generating drawings. (2) The difference in skills between workers preparing drawings has been eliminated and the quality of drawings has been made uniform. (3) The extent of knowledge and experience demanded of workers has been reduced. (author)

  14. Terminal automation system maintenance

    Energy Technology Data Exchange (ETDEWEB)

    Coffelt, D.; Hewitt, J. [Engineered Systems Inc., Tempe, AZ (United States)

    1997-01-01

    Nothing has improved petroleum product loading in recent years more than terminal automation systems. The presence of terminal automation systems (TAS) at loading racks has increased operational efficiency and safety and enhanced their accounting and management capabilities. However, like all finite systems, they occasionally malfunction or fail. Proper servicing and maintenance can minimize this. And in the unlikely event a TAS breakdown does occur, prompt and effective troubleshooting can reduce its impact on terminal productivity. To accommodate around-the-clock loading at racks, increasingly unattended by terminal personnel, TAS maintenance, servicing and troubleshooting has become increasingly demanding. It has also become increasingly important. After 15 years of trial and error at petroleum and petrochemical storage and transfer terminals, a number of successful troubleshooting programs have been developed. These include 24-hour {open_quotes}help hotlines,{close_quotes} internal (terminal company) and external (supplier) support staff, and {open_quotes}layered{close_quotes} support. These programs are described.

  15. ATLAS Distributed Computing Automation

    CERN Document Server

    Schovancova, J; The ATLAS collaboration; Borrego, C; Campana, S; Di Girolamo, A; Elmsheuser, J; Hejbal, J; Kouba, T; Legger, F; Magradze, E; Medrano Llamas, R; Negri, G; Rinaldi, L; Sciacca, G; Serfon, C; Van Der Ster, D C

    2012-01-01

    The ATLAS Experiment benefits from computing resources distributed worldwide at more than 100 WLCG sites. The ATLAS Grid sites provide over 100k CPU job slots, over 100 PB of storage space on disk or tape. Monitoring of status of such a complex infrastructure is essential. The ATLAS Grid infrastructure is monitored 24/7 by two teams of shifters distributed world-wide, by the ATLAS Distributed Computing experts, and by site administrators. In this paper we summarize automation efforts performed within the ATLAS Distributed Computing team in order to reduce manpower costs and improve the reliability of the system. Different aspects of the automation process are described: from the ATLAS Grid site topology provided by the ATLAS Grid Information System, via automatic site testing by the HammerCloud, to automatic exclusion from production or analysis activities.

  16. Rapid automated nuclear chemistry

    International Nuclear Information System (INIS)

    Rapid Automated Nuclear Chemistry (RANC) can be thought of as the Z-separation of Neutron-rich Isotopes by Automated Methods. The range of RANC studies of fission and its products is large. In a sense, the studies can be categorized into various energy ranges from the highest where the fission process and particle emission are considered, to low energies where nuclear dynamics are being explored. This paper presents a table which gives examples of current research using RANC on fission and fission products. The remainder of this text is divided into three parts. The first contains a discussion of the chemical methods available for the fission product elements, the second describes the major techniques, and in the last section, examples of recent results are discussed as illustrations of the use of RANC

  17. Rapid automated nuclear chemistry

    Energy Technology Data Exchange (ETDEWEB)

    Meyer, R.A.

    1979-05-31

    Rapid Automated Nuclear Chemistry (RANC) can be thought of as the Z-separation of Neutron-rich Isotopes by Automated Methods. The range of RANC studies of fission and its products is large. In a sense, the studies can be categorized into various energy ranges from the highest where the fission process and particle emission are considered, to low energies where nuclear dynamics are being explored. This paper presents a table which gives examples of current research using RANC on fission and fission products. The remainder of this text is divided into three parts. The first contains a discussion of the chemical methods available for the fission product elements, the second describes the major techniques, and in the last section, examples of recent results are discussed as illustrations of the use of RANC.

  18. Automated Microbial Metabolism Laboratory

    Science.gov (United States)

    1973-01-01

    Development of the automated microbial metabolism laboratory (AMML) concept is reported. The focus of effort of AMML was on the advanced labeled release experiment. Labeled substrates, inhibitors, and temperatures were investigated to establish a comparative biochemical profile. Profiles at three time intervals on soil and pure cultures of bacteria isolated from soil were prepared to establish a complete library. The development of a strategy for the return of a soil sample from Mars is also reported.

  19. Components for automated microscopy

    Science.gov (United States)

    Determann, H.; Hartmann, H.; Schade, K. H.; Stankewitz, H. W.

    1980-12-01

    A number of devices, aiming at automated analysis of microscopic objects as regards their morphometrical parameters or their photometrical values, were developed. These comprise: (1) a device for automatic focusing tuned on maximum contrast; (2) a feedback system for automatic optimization of microscope illumination; and (3) microscope lenses with adjustable pupil distances for usage in the two previous devices. An extensive test program on histological and zytological applications proves the wide application possibilities of the autofocusing device.

  20. Automation of dissolution tests

    OpenAIRE

    Rolf Rolli

    2003-01-01

    Dissolution testing of drug formulations was introduced in the 1960s and accepted by health regulatory authorities in the 1970s. Since then, the importance of dissolution has grown rapidly as have the number of tests and demands in quality-control laboratories. Recent research works lead to the development of in-vitro dissolution tests as replacements for human and animal bioequivalence studies. For many years, a lot of time and effort has been invested in automation of dissolution tests. The...

  1. Automated uranium assays

    International Nuclear Information System (INIS)

    Precise, timely inventories of enriched uranium stocks are vital to help prevent the loss, theft, or diversion of this material for illicit use. A wet-chemistry analyzer has been developed at LLL to assist in these inventories by performing automated analyses of uranium samples from different stages in the nuclear fuel cycle. These assays offer improved accuracy, reduced costs, significant savings in manpower, and lower radiation exposure for personnel compared with present techniques

  2. Construction Automation and Robotics

    OpenAIRE

    Bock, Thomas

    2008-01-01

    Due to the high complexity of the construction process and the stagnating technological development a long-term preparation is necessary to adapt it to advanced construction methods. Architects, engineers and all other participants of the construction process have to be integrated in this adaptation process. The short- and long-term development of automation will take place step-by-step and will be oriented to the respective application and requirements. In the initial phase existing building...

  3. Shielded cells transfer automation

    International Nuclear Information System (INIS)

    Nuclear waste from shielded cells is removed, packaged, and transferred manually in many nuclear facilities. To reduce radiation exposure to operators, technological advances in remote handling and automation were employed. An industrial robot and a specially designed end effector, access port, and sealing machine were used to remotely bag waste containers out of a glove box. The system is operated from a control panel outside the work area via television cameras

  4. LINAC control automation system

    International Nuclear Information System (INIS)

    A 7 MeV Electron Beam Linear Accelerator (LINAC) being used for pulse radiolysis experiments at RC and CDD, B.A.R.C. has been automated with a PLC based control panel designed and developed by Computer Division, B.A.R.C.. The control panel after power on switches ON various units in a pre-defined sequence and intervals on a single turn of START key from OFF to ON position. The control panel also generates various ramp signals in a pre-defined sequence and rate and steady values and feeds to the LINAC bringing it to the ready for experiment condition. Similarly on a single turn of STOP key from OFF to ON position, the panel ramps down the various signals in pre-defined manners and makes OFF the various units in predefined sequence and timing providing safety to the machine. The steady values for various signals are on line settable as and when required so. This automation system relieves the operator from fatigue of time consuming manual ramping up or down of various signals and running around in four rooms for switching ON or OFF the various units enhancing efficiency and safety. This also facilitates the user scientist to do start up and shutdown operation in the absence of skilled operators and thus adds flexibility for working up to extended timing. This unit has been working satisfactorily since August 2002. For extraordinary condition automation to manual or vice versa change over has been provided. (author)

  5. Perfect Actions for Scalar Theories

    CERN Document Server

    Bietenholz, W

    1998-01-01

    We construct an optimally local perfect lattice action for free scalars of arbitrary mass, and truncate its couplings to a unit hypercube. Spectral and thermodynamic properties of this ``hypercube scalar'' are drastically improved compared to the standard action. We also discuss new variants of perfect actions, using anisotropic of triangular lattices, or applying new types of RGTs. Finally we add a $\\lambda \\phi^{4}$ term and address perfect lattice perturbation theory. We report on a lattice action for the anharmonic oscillator, which is perfect to $O(\\lambda)$.

  6. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (July--September 1990) and includes copies of letters, notices, and orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  7. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (January--March 1991) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  8. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (October--December 1992) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  9. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (October--December 1990) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  10. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (January--March 1992) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  11. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (April-June 1991) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  12. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (July--September 1989) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  13. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (October--December 1991) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  14. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (January--March 1993) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  15. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (April--June 1990) and includes copies of letters, notices, and orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  16. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (April--June 1993) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  17. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (July--September 1991) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  18. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (July--September 1993) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  19. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (October--December 1989) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  20. Enforcement actions: Significant actions resolved

    International Nuclear Information System (INIS)

    This compilation summarizes significant enforcement actions that have been resolved during one quarterly period (April--June 1992) and includes copies of letters, Notices, and Orders sent by the Nuclear Regulatory Commission to licensees with respect to these enforcement actions. It is anticipated that the information in this publication will be widely disseminated to managers and employees engaged in activities licensed by the NRC, so that actions can be taken to improve safety by avoiding future violations similar to those described in this publication

  1. ALLocator: an interactive web platform for the analysis of metabolomic LC-ESI-MS datasets, enabling semi-automated, user-revised compound annotation and mass isotopomer ratio analysis.

    Directory of Open Access Journals (Sweden)

    Nikolas Kessler

    Full Text Available Adduct formation, fragmentation events and matrix effects impose special challenges to the identification and quantitation of metabolites in LC-ESI-MS datasets. An important step in compound identification is the deconvolution of mass signals. During this processing step, peaks representing adducts, fragments, and isotopologues of the same analyte are allocated to a distinct group, in order to separate peaks from coeluting compounds. From these peak groups, neutral masses and pseudo spectra are derived and used for metabolite identification via mass decomposition and database matching. Quantitation of metabolites is hampered by matrix effects and nonlinear responses in LC-ESI-MS measurements. A common approach to correct for these effects is the addition of a U-13C-labeled internal standard and the calculation of mass isotopomer ratios for each metabolite. Here we present a new web-platform for the analysis of LC-ESI-MS experiments. ALLocator covers the workflow from raw data processing to metabolite identification and mass isotopomer ratio analysis. The integrated processing pipeline for spectra deconvolution "ALLocatorSD" generates pseudo spectra and automatically identifies peaks emerging from the U-13C-labeled internal standard. Information from the latter improves mass decomposition and annotation of neutral losses. ALLocator provides an interactive and dynamic interface to explore and enhance the results in depth. Pseudo spectra of identified metabolites can be stored in user- and method-specific reference lists that can be applied on succeeding datasets. The potential of the software is exemplified in an experiment, in which abundance fold-changes of metabolites of the l-arginine biosynthesis in C. glutamicum type strain ATCC 13032 and l-arginine producing strain ATCC 21831 are compared. Furthermore, the capability for detection and annotation of uncommon large neutral losses is shown by the identification of (γ-glutamyl dipeptides in

  2. A New Approach to an Automated Air Traffic Control

    Institute of Scientific and Technical Information of China (English)

    Patchev Dragoljub

    2014-01-01

    This paper identifies areas of improvements of the air traffic control system and proposes modification of the concept of automation by using available technologies. With the proposed modification, the current Europe wide en route network structure can be modified in order to make routes more optimal. For this new route network structure, a new concept of automation will be used to manage with the air traffic. The first identified area of improvement is implementation of automation process that will enable decentralization of the air traffic control functionality to each individual aircraft and this will be achieved through automated routing of the aircrafts and CD&R (conflict detection and resolution). The FMS (flight management system) at the aircraft will make decisions for the optimal flight route based on the sensor inputs, information on selection of the routes, next hope points and flight levels, all received by ADS-B (automatic dependant surveillance-broadcast). The second area is processing the information about the deviation from the optimal route as in flight plan due to a traffic management (vectoring, level change) and taking it into consideration when further actions are undertaken. For each action, a cost factor will be calculated from the fuel burned for that action. This factor will be used to select conflict resolution protocol. The proposed concept shall increase the capacity of the network, and enable the air traff~c more efficient and more environmentally friendly while maintaining safe separation.

  3. M2m Automation: Matlab-To-Map Reduce Automation

    Directory of Open Access Journals (Sweden)

    Archana C S

    2014-06-01

    Full Text Available Abstract- MapReduce is a very popular parallel programming model for cloud computing platforms, and has become an effective method for processing massive data by using a cluster of computers. Program language -to-MapReduce Automator is a possible solution to help traditional programmers easily deploy an application to cloud systems through translating sequential codes to MapReduce codes.M2M Automation mainly focuses on automating numerical computations by using hadoop at the back end. M2M automates Hadoop, for faster execution of Matlab commands using MapReduce code.

  4. Corrective actions

    International Nuclear Information System (INIS)

    The variety of corrective actions which have been attempted at many radioactive waste disposal sites points to less than ideal performance by present-day standards at many closed and presently-operating sites. In humid regions, most of the problems have encompassed some kind of water intrusion into the buried waste. In arid regions, the problems have centered on trench subsidence and intrusion by plant roots and animals. It is overwhelmingly apparent that any protective barrier for the buried waste, whether for water or biological intrusion, will depend on stable support from the underlying burial trenches. Trench subsidence must be halted, prevented, or circumscribed in some manner to assure this necessary long-term support. Final corrective actions will differ considerably from site to site, depending on unique geological, pedological, and meteorological environments. In the meantime, many of the shorter-term corrective actions described in this chapter can be implemented as immediate needs dictate

  5. Automated Assessment, Face to Face

    OpenAIRE

    Rizik M. H. Al-Sayyed; Amjad Hudaib; Muhannad AL-Shboul; Yousef Majdalawi; Mohammed Bataineh

    2010-01-01

    This research paper evaluates the usability of automated exams and compares them with the paper-and-pencil traditional ones. It presents the results of a detailed study conducted at The University of Jordan (UoJ) that comprised students from 15 faculties. A set of 613 students were asked about their opinions concerning automated exams; and their opinions were deeply analyzed. The results indicate that most students reported that they are satisfied with using automated exams but they have sugg...

  6. Automation System Products and Research

    OpenAIRE

    Rintala, Mikko; Sormunen, Jussi; Kuisma, Petri; Rahkala, Matti

    2014-01-01

    Automation systems are used in most buildings nowadays. In the past they were mainly used in industry to control and monitor critical systems. During the past few decades the automation systems have become more common and are used today from big industrial solutions to homes of private customers. With the growing need for ecologic and cost-efficient management systems, home and building automation systems are becoming a standard way of controlling lighting, ventilation, heating etc. Auto...

  7. Software Testing and Documenting Automation

    OpenAIRE

    Tsybin, Anton; Lyadova, Lyudmila

    2008-01-01

    This article describes some approaches to problem of testing and documenting automation in information systems with graphical user interface. Combination of data mining methods and theory of finite state machines is used for testing automation. Automated creation of software documentation is based on using metadata in documented system. Metadata is built on graph model. Described approaches improve performance and quality of testing and documenting processes.

  8. Embedded system for building automation

    OpenAIRE

    Rolih, Andrej

    2014-01-01

    Home automation is a fast developing field of computer science and electronics. Companies are offering many different products for home automation. Ranging anywhere from complete systems for building management and control, to simple smart lights that can be connected to the internet. These products offer the user greater living comfort and lower their expenses by reducing the energy usage. This thesis shows the development of a simple home automation system that focuses mainly on the enhance...

  9. World-wide distribution automation systems

    Energy Technology Data Exchange (ETDEWEB)

    Devaney, T.M.

    1994-12-31

    A worldwide power distribution automation system is outlined. Distribution automation is defined and the status of utility automation is discussed. Other topics discussed include a distribution management system, substation feeder, and customer functions, potential benefits, automation costs, planning and engineering considerations, automation trends, databases, system operation, computer modeling of system, and distribution management systems.

  10. AUTOMATED API TESTING APPROACH

    Directory of Open Access Journals (Sweden)

    SUNIL L. BANGARE

    2012-02-01

    Full Text Available Software testing is an investigation conducted to provide stakeholders with information about the quality of the product or service under test. With the help of software testing we can verify or validate the software product. Normally testing will be done after development of software but we can perform the software testing at the time of development process also. This paper will give you a brief introduction about Automated API Testing Tool. This tool of testing will reduce lots of headache after the whole development of software. It saves time as well as money. Such type of testing is helpful in the Industries & Colleges also.

  11. Automated radioimmunoassay of nicotine

    International Nuclear Information System (INIS)

    The authors have developed an automated nonequilibrium procedure for the radioimmunoassay of nicotine. The use of a unique iodinated nicotine derivative in this procedure gave a sensitivity of 10 μg/l for nicotine with a between-run precision of 7.4% and within-run precision of 6.0%. Nicotine levels of 60 to 67 μg/l were found in subjects 15 min after smoking one standard cigarette. The technique herein reported is a very rapid, and sensitive radioimmunoassay for nicotine and facilitates the determination of nicotine in smoking subjects during the actual process of smoking. (Auth.)

  12. Automated Motivic Analysis

    DEFF Research Database (Denmark)

    Lartillot, Olivier

    2016-01-01

    Motivic analysis provides very detailed understanding of musical composi- tions, but is also particularly difficult to formalize and systematize. A computational automation of the discovery of motivic patterns cannot be reduced to a mere extraction of all possible sequences of descriptions....... The systematic approach inexorably leads to a proliferation of redundant structures that needs to be addressed properly. Global filtering techniques cause a drastic elimination of interesting structures that damages the quality of the analysis. On the other hand, a selection of closed patterns allows...

  13. Mechatronic Design Automation

    DEFF Research Database (Denmark)

    Fan, Zhun

    This book proposes a novel design method that combines both genetic programming (GP) to automatically explore the open-ended design space and bond graphs (BG) to unify design representations of multi-domain Mechatronic systems. Results show that the method, formally called GPBG method, can...... successfully design analogue filters, vibration absorbers, micro-electro-mechanical systems, and vehicle suspension systems, all in an automatic or semi-automatic way. It also investigates the very important issue of co-designing plant-structures and dynamic controllers in automated design of Mechatronic...

  14. AUTOMATED PROCESS MONITORING: APPLYING PROVEN AUTOMATION TECHNIQUES TO INTERNATIONAL SAFEGUARDS NEEDS

    International Nuclear Information System (INIS)

    Identification and quantification of specific alpha- and beta-emitting radionuclides in complex liquid matrices is highly challenging, and is typically accomplished through laborious wet chemical sample preparation and separations followed by analysis using a variety of detection methodologies (e.g., liquid scintillation, gas proportional counting, alpha energy analysis, mass spectrometry). Analytical results may take days or weeks to report. Chains of custody and sample security measures may also complicate or slow the analytical process. When an industrial process-scale plant requires the monitoring of specific radionuclides as an indication of the composition of its feed stream or of plant performance, radiochemical measurements must be fast, accurate, and reliable. Scientists at Pacific Northwest National Laboratory have assembled a fully automated prototype Process Monitor instrument capable of a variety of tasks: automated sampling directly from a feed stream, sample digestion/analyte redox adjustment, chemical separations, radiochemical detection and data analysis/reporting. The system is compact, its components are fluidically inter-linked, and analytical results could be immediately transmitted to on- or off-site locations. The development of a rapid radiochemical Process Monitor for 99Tc in Hanford tank waste processing streams, capable of performing several measurements per hour, will be discussed in detail. More recently, the automated platform was modified to perform measurements of 90Sr in Hanford tank waste stimulant. The system exemplifies how automation could be integrated into reprocessing facilities to support international nuclear safeguards needs

  15. Automating the Production of Interactive Technical Documentation and EPSS.

    Science.gov (United States)

    Emmott, Lyle C.

    1998-01-01

    Discusses how the production of IETM (interactive electronic technical manuals) and EPSS (electronic performance support systems) has been automated to give subject-matter experts the ability to mass-produce electronic documents, training, and training-support materials. Describes four projects involving object-relational database architecture.…

  16. A computational framework for the automated construction of glycosylation reaction networks.

    Directory of Open Access Journals (Sweden)

    Gang Liu

    Full Text Available Glycosylation is among the most common and complex post-translational modifications identified to date. It proceeds through the catalytic action of multiple enzyme families that include the glycosyltransferases that add monosaccharides to growing glycans, and glycosidases which remove sugar residues to trim glycans. The expression level and specificity of these enzymes, in part, regulate the glycan distribution or glycome of specific cell/tissue systems. Currently, there is no systematic method to describe the enzymes and cellular reaction networks that catalyze glycosylation. To address this limitation, we present a streamlined machine-readable definition for the glycosylating enzymes and additional methodologies to construct and analyze glycosylation reaction networks. In this computational framework, the enzyme class is systematically designed to store detailed specificity data such as enzymatic functional group, linkage and substrate specificity. The new classes and their associated functions enable both single-reaction inference and automated full network reconstruction, when given a list of reactants and/or products along with the enzymes present in the system. In addition, graph theory is used to support functions that map the connectivity between two or more species in a network, and that generate subset models to identify rate-limiting steps regulating glycan biosynthesis. Finally, this framework allows the synthesis of biochemical reaction networks using mass spectrometry (MS data. The features described above are illustrated using three case studies that examine: i O-linked glycan biosynthesis during the construction of functional selectin-ligands; ii automated N-linked glycosylation pathway construction; and iii the handling and analysis of glycomics based MS data. Overall, the new computational framework enables automated glycosylation network model construction and analysis by integrating knowledge of glycan structure and enzyme

  17. Action Research

    Science.gov (United States)

    Milton-Brkich, Katie Lynn; Shumbera, Kristen; Beran, Becky

    2010-01-01

    Defined as "any systemic inquiry conducted by teachers... for the purpose of gathering information about how their particular schools operate, how they teach, and how their students learn" (Mertler, 2009), "action research" is empowering and professional research done by teachers to inform and improves their own practices. Although there are many…

  18. China's Actions

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ China's National Development and Reform Commission publicized the country's policies and actions for addressing climate change in a report released on November 26,2009.The report highlighted China's efforts in cutting greenhouse gas emissions in 2009 by: (1)Rigorously checking the blind expansion of its energy-and pollution-intensive industries.

  19. Maneuver Automation Software

    Science.gov (United States)

    Uffelman, Hal; Goodson, Troy; Pellegrin, Michael; Stavert, Lynn; Burk, Thomas; Beach, David; Signorelli, Joel; Jones, Jeremy; Hahn, Yungsun; Attiyah, Ahlam; Illsley, Jeannette

    2009-01-01

    The Maneuver Automation Software (MAS) automates the process of generating commands for maneuvers to keep the spacecraft of the Cassini-Huygens mission on a predetermined prime mission trajectory. Before MAS became available, a team of approximately 10 members had to work about two weeks to design, test, and implement each maneuver in a process that involved running many maneuver-related application programs and then serially handing off data products to other parts of the team. MAS enables a three-member team to design, test, and implement a maneuver in about one-half hour after Navigation has process-tracking data. MAS accepts more than 60 parameters and 22 files as input directly from users. MAS consists of Practical Extraction and Reporting Language (PERL) scripts that link, sequence, and execute the maneuver- related application programs: "Pushing a single button" on a graphical user interface causes MAS to run navigation programs that design a maneuver; programs that create sequences of commands to execute the maneuver on the spacecraft; and a program that generates predictions about maneuver performance and generates reports and other files that enable users to quickly review and verify the maneuver design. MAS can also generate presentation materials, initiate electronic command request forms, and archive all data products for future reference.

  20. Automated Test Case Generation

    CERN Document Server

    CERN. Geneva

    2015-01-01

    I would like to present the concept of automated test case generation. I work on it as part of my PhD and I think it would be interesting also for other people. It is also the topic of a workshop paper that I am introducing in Paris. (abstract below) Please note that the talk itself would be more general and not about the specifics of my PhD, but about the broad field of Automated Test Case Generation. I would introduce the main approaches (combinatorial testing, symbolic execution, adaptive random testing) and their advantages and problems. (oracle problem, combinatorial explosion, ...) Abstract of the paper: Over the last decade code-based test case generation techniques such as combinatorial testing or dynamic symbolic execution have seen growing research popularity. Most algorithms and tool implementations are based on finding assignments for input parameter values in order to maximise the execution branch coverage. Only few of them consider dependencies from outside the Code Under Test’s scope such...

  1. Automated Postediting of Documents

    CERN Document Server

    Knight, K; Knight, Kevin; Chander, Ishwar

    1994-01-01

    Large amounts of low- to medium-quality English texts are now being produced by machine translation (MT) systems, optical character readers (OCR), and non-native speakers of English. Most of this text must be postedited by hand before it sees the light of day. Improving text quality is tedious work, but its automation has not received much research attention. Anyone who has postedited a technical report or thesis written by a non-native speaker of English knows the potential of an automated postediting system. For the case of MT-generated text, we argue for the construction of postediting modules that are portable across MT systems, as an alternative to hardcoding improvements inside any one system. As an example, we have built a complete self-contained postediting module for the task of article selection (a, an, the) for English noun phrases. This is a notoriously difficult problem for Japanese-English MT. Our system contains over 200,000 rules derived automatically from online text resources. We report on l...

  2. Automated Functional Testing based on the Navigation of Web Applications

    CERN Document Server

    García, Boni; 10.4204/EPTCS.61.4

    2011-01-01

    Web applications are becoming more and more complex. Testing such applications is an intricate hard and time-consuming activity. Therefore, testing is often poorly performed or skipped by practitioners. Test automation can help to avoid this situation. Hence, this paper presents a novel approach to perform automated software testing for web applications based on its navigation. On the one hand, web navigation is the process of traversing a web application using a browser. On the other hand, functional requirements are actions that an application must do. Therefore, the evaluation of the correct navigation of web applications results in the assessment of the specified functional requirements. The proposed method to perform the automation is done in four levels: test case generation, test data derivation, test case execution, and test case reporting. This method is driven by three kinds of inputs: i) UML models; ii) Selenium scripts; iii) XML files. We have implemented our approach in an open-source testing fra...

  3. Current advances and strategies towards fully automated sample preparation for regulated LC-MS/MS bioanalysis.

    Science.gov (United States)

    Zheng, Naiyu; Jiang, Hao; Zeng, Jianing

    2014-09-01

    Robotic liquid handlers (RLHs) have been widely used in automated sample preparation for liquid chromatography-tandem mass spectrometry (LC-MS/MS) bioanalysis. Automated sample preparation for regulated bioanalysis offers significantly higher assay efficiency, better data quality and potential bioanalytical cost-savings. For RLHs that are used for regulated bioanalysis, there are additional requirements, including 21 CFR Part 11 compliance, software validation, system qualification, calibration verification and proper maintenance. This article reviews recent advances in automated sample preparation for regulated bioanalysis in the last 5 years. Specifically, it covers the following aspects: regulated bioanalysis requirements, recent advances in automation hardware and software development, sample extraction workflow simplification, strategies towards fully automated sample extraction, and best practices in automated sample preparation for regulated bioanalysis. PMID:25384595

  4. Free viewpoint action recognition using motion history volumes

    OpenAIRE

    Weinland, Daniel; Ronfard, Rémi; Ronfard, Remi; Boyer, Edmond

    2006-01-01

    Action recognition is an important and challenging topic in computer vision, with many important applications including video surveillance, automated cinematography and understanding of social interaction. Yet, most current work in gesture or action interpretation remains rooted in view-dependent representations. This paper introduces Motion History Volumes (MHV) as a free-viewpoint representation for human actions in the case of multiple calibrated, and background-subtracted, video cameras. ...

  5. Testing automation of projects in telecommunication domain

    OpenAIRE

    Alexey, Veselov; Vsevolod, Kotlyarov

    2010-01-01

    This paper presents an integrated approach to testing automation of telecommunication projects along with proposals to automation of conformance testing. The underlying idea is to benefit from combining formal verification and testing automation techniques in order to improve product quality.

  6. Least-action perihelion precession

    CERN Document Server

    Koskela, Mikael

    2010-01-01

    The precession of Mercury's perihelion is reinspected by the principle of least action. The anomalous advancement of the apside line that is customarily accounted by the theory of general relativity, is ascribed to the gravitational effect due to the entire Universe. When the least action is written in the Sun's frame of reference, the residual rotation is seen to stem from inertia due to all bodies in the Universe. Since mass corresponds to a bound form of energy, gravity, as any other force, can be described as an energy density difference between a system of bodies and its surrounding energy densities that are dispersed throughout the Universe. According to the principle of least action the Universe is expanding by combustion of mass to radiation in the quest of equilibrating the bound forms of energy with "zero-density surroundings" in least time. Keywords: cosmological principle; energy density; energy dispersal; evolution; gravity; the principle of least action

  7. Automating usability of ATLAS Distributed Computing resources

    Science.gov (United States)

    Tupputi, S. A.; Di Girolamo, A.; Kouba, T.; Schovancová, J.; Atlas Collaboration

    2014-06-01

    The automation of ATLAS Distributed Computing (ADC) operations is essential to reduce manpower costs and allow performance-enhancing actions, which improve the reliability of the system. In this perspective a crucial case is the automatic handling of outages of ATLAS computing sites storage resources, which are continuously exploited at the edge of their capabilities. It is challenging to adopt unambiguous decision criteria for storage resources of non-homogeneous types, sizes and roles. The recently developed Storage Area Automatic Blacklisting (SAAB) tool has provided a suitable solution, by employing an inference algorithm which processes history of storage monitoring tests outcome. SAAB accomplishes both the tasks of providing global monitoring as well as automatic operations on single sites. The implementation of the SAAB tool has been the first step in a comprehensive review of the storage areas monitoring and central management at all levels. Such review has involved the reordering and optimization of SAM tests deployment and the inclusion of SAAB results in the ATLAS Site Status Board with both dedicated metrics and views. The resulting structure allows monitoring the storage resources status with fine time-granularity and automatic actions to be taken in foreseen cases, like automatic outage handling and notifications to sites. Hence, the human actions are restricted to reporting and following up problems, where and when needed. In this work we show SAAB working principles and features. We present also the decrease of human interactions achieved within the ATLAS Computing Operation team. The automation results in a prompt reaction to failures, which leads to the optimization of resource exploitation.

  8. Automating usability of ATLAS distributed computing resources

    International Nuclear Information System (INIS)

    The automation of ATLAS Distributed Computing (ADC) operations is essential to reduce manpower costs and allow performance-enhancing actions, which improve the reliability of the system. In this perspective a crucial case is the automatic handling of outages of ATLAS computing sites storage resources, which are continuously exploited at the edge of their capabilities. It is challenging to adopt unambiguous decision criteria for storage resources of non-homogeneous types, sizes and roles. The recently developed Storage Area Automatic Blacklisting (SAAB) tool has provided a suitable solution, by employing an inference algorithm which processes history of storage monitoring tests outcome. SAAB accomplishes both the tasks of providing global monitoring as well as automatic operations on single sites. The implementation of the SAAB tool has been the first step in a comprehensive review of the storage areas monitoring and central management at all levels. Such review has involved the reordering and optimization of SAM tests deployment and the inclusion of SAAB results in the ATLAS Site Status Board with both dedicated metrics and views. The resulting structure allows monitoring the storage resources status with fine time-granularity and automatic actions to be taken in foreseen cases, like automatic outage handling and notifications to sites. Hence, the human actions are restricted to reporting and following up problems, where and when needed. In this work we show SAAB working principles and features. We present also the decrease of human interactions achieved within the ATLAS Computing Operation team. The automation results in a prompt reaction to failures, which leads to the optimization of resource exploitation.

  9. Automated Methods of Corrosion Measurements

    DEFF Research Database (Denmark)

    Andersen, Jens Enevold Thaulov

    . Mechanical control, recording, and data processing must therefore be automated to a high level of precision and reliability. These general techniques and the apparatus involved have been described extensively. The automated methods of such high-resolution microscopy coordinated with computerized...

  10. Opening up Library Automation Software

    Science.gov (United States)

    Breeding, Marshall

    2009-01-01

    Throughout the history of library automation, the author has seen a steady advancement toward more open systems. In the early days of library automation, when proprietary systems dominated, the need for standards was paramount since other means of inter-operability and data exchange weren't possible. Today's focus on Application Programming…

  11. Automation, Performance and International Competition

    DEFF Research Database (Denmark)

    Kromann, Lene; Sørensen, Anders

    productivity growth than other firms. Moreover, automation improves the efficiency of all stages of the production process by reducing setup time, run time, and inspection time and increasing uptime and quantity produced per worker. The efficiency improvement varies by type of automation....

  12. Automated separation for heterogeneous immunoassays

    OpenAIRE

    Truchaud, A.; Barclay, J; Yvert, J. P.; Capolaghi, B.

    1991-01-01

    Beside general requirements for modern automated systems, immunoassay automation involves specific requirements as a separation step for heterogeneous immunoassays. Systems are designed according to the solid phase selected: dedicated or open robots for coated tubes and wells, systems nearly similar to chemistry analysers in the case of magnetic particles, and a completely original design for those using porous and film materials.

  13. Automated Test-Form Generation

    Science.gov (United States)

    van der Linden, Wim J.; Diao, Qi

    2011-01-01

    In automated test assembly (ATA), the methodology of mixed-integer programming is used to select test items from an item bank to meet the specifications for a desired test form and optimize its measurement accuracy. The same methodology can be used to automate the formatting of the set of selected items into the actual test form. Three different…

  14. Automated Methods Of Corrosion Measurements

    DEFF Research Database (Denmark)

    Bech-Nielsen, Gregers; Andersen, Jens Enevold Thaulov; Reeve, John Ch;

    1997-01-01

    The chapter describes the following automated measurements: Corrosion Measurements by Titration, Imaging Corrosion by Scanning Probe Microscopy, Critical Pitting Temperature and Application of the Electrochemical Hydrogen Permeation Cell.......The chapter describes the following automated measurements: Corrosion Measurements by Titration, Imaging Corrosion by Scanning Probe Microscopy, Critical Pitting Temperature and Application of the Electrochemical Hydrogen Permeation Cell....

  15. Automated Standard Hazard Tool

    Science.gov (United States)

    Stebler, Shane

    2014-01-01

    The current system used to generate standard hazard reports is considered cumbersome and iterative. This study defines a structure for this system's process in a clear, algorithmic way so that standard hazard reports and basic hazard analysis may be completed using a centralized, web-based computer application. To accomplish this task, a test server is used to host a prototype of the tool during development. The prototype is configured to easily integrate into NASA's current server systems with minimal alteration. Additionally, the tool is easily updated and provides NASA with a system that may grow to accommodate future requirements and possibly, different applications. Results of this project's success are outlined in positive, subjective reviews complete by payload providers and NASA Safety and Mission Assurance personnel. Ideally, this prototype will increase interest in the concept of standard hazard automation and lead to the full-scale production of a user-ready application.

  16. Expedition automated flow fluorometer

    Science.gov (United States)

    Krikun, V. A.; Salyuk, P. A.

    2015-11-01

    This paper describes an apparatus and operation of automated flow-through dual-channel fluorometer for studying the fluorescence of dissolved organic matter, and the fluorescence of phytoplankton cells with open and closed reaction centers in sea areas with oligotrophic and eutrophic water type. The step-by step excitation by two semiconductor lasers or two light-emitting diodes is realized in the current device. The excitation wavelengths are 405nm and 532nm in the default configuration. Excitation radiation of each light source can be changed with different durations, intensities and repetition rate. Registration of the fluorescence signal carried out by two photo-multipliers with different optical filters of 580-600 nm and 680-700 nm band pass diapasons. The configuration of excitation sources and spectral diapasons of registered radiation can be changed due to decided tasks.

  17. Robust automated knowledge capture.

    Energy Technology Data Exchange (ETDEWEB)

    Stevens-Adams, Susan Marie; Abbott, Robert G.; Forsythe, James Chris; Trumbo, Michael Christopher Stefan; Haass, Michael Joseph; Hendrickson, Stacey M. Langfitt

    2011-10-01

    This report summarizes research conducted through the Sandia National Laboratories Robust Automated Knowledge Capture Laboratory Directed Research and Development project. The objective of this project was to advance scientific understanding of the influence of individual cognitive attributes on decision making. The project has developed a quantitative model known as RumRunner that has proven effective in predicting the propensity of an individual to shift strategies on the basis of task and experience related parameters. Three separate studies are described which have validated the basic RumRunner model. This work provides a basis for better understanding human decision making in high consequent national security applications, and in particular, the individual characteristics that underlie adaptive thinking.

  18. Berkeley automated supernova search

    International Nuclear Information System (INIS)

    The Berkeley automated supernova search employs a computer controlled 36-inch telescope and charge coupled device (CCD) detector to image 2500 galaxies per night. A dedicated minicomputer compares each galaxy image with stored reference data to identify supernovae in real time. The threshold for detection is m/sub v/ = 18.8. We plan to monitor roughly 500 galaxies in Virgo and closer every night, and an additional 6000 galaxies out to 70 Mpc on a three night cycle. This should yield very early detection of several supernovae per year for detailed study, and reliable premaximum detection of roughly 100 supernovae per year for statistical studies. The search should be operational in mid-1982

  19. Automated synthetic scene generation

    Science.gov (United States)

    Givens, Ryan N.

    Physics-based simulations generate synthetic imagery to help organizations anticipate system performance of proposed remote sensing systems. However, manually constructing synthetic scenes which are sophisticated enough to capture the complexity of real-world sites can take days to months depending on the size of the site and desired fidelity of the scene. This research, sponsored by the Air Force Research Laboratory's Sensors Directorate, successfully developed an automated approach to fuse high-resolution RGB imagery, lidar data, and hyperspectral imagery and then extract the necessary scene components. The method greatly reduces the time and money required to generate realistic synthetic scenes and developed new approaches to improve material identification using information from all three of the input datasets.

  20. Automated Electrostatics Environmental Chamber

    Science.gov (United States)

    Calle, Carlos; Lewis, Dean C.; Buchanan, Randy K.; Buchanan, Aubri

    2005-01-01

    The Mars Electrostatics Chamber (MEC) is an environmental chamber designed primarily to create atmospheric conditions like those at the surface of Mars to support experiments on electrostatic effects in the Martian environment. The chamber is equipped with a vacuum system, a cryogenic cooling system, an atmospheric-gas replenishing and analysis system, and a computerized control system that can be programmed by the user and that provides both automation and options for manual control. The control system can be set to maintain steady Mars-like conditions or to impose temperature and pressure variations of a Mars diurnal cycle at any given season and latitude. In addition, the MEC can be used in other areas of research because it can create steady or varying atmospheric conditions anywhere within the wide temperature, pressure, and composition ranges between the extremes of Mars-like and Earth-like conditions.

  1. [From automation to robotics].

    Science.gov (United States)

    1985-01-01

    The introduction of automation into the laboratory of biology seems to be unavoidable. But at which cost, if it is necessary to purchase a new machine for every new application? Fortunately the same image processing techniques, belonging to a theoretic framework called Mathematical Morphology, may be used in visual inspection tasks, both in car industry and in the biology lab. Since the market for industrial robotics applications is much higher than the market of biomedical applications, the price of image processing devices drops, and becomes sometimes less than the price of a complete microscope equipment. The power of the image processing methods of Mathematical Morphology will be illustrated by various examples, as automatic silver grain counting in autoradiography, determination of HLA genotype, electrophoretic gels analysis, automatic screening of cervical smears... Thus several heterogeneous applications may share the same image processing device, provided there is a separate and devoted work station for each of them. PMID:4091303

  2. Automating the multiprocessing environment

    Science.gov (United States)

    Arpasi, Dale J.

    1989-01-01

    An approach to automate the programming and operation of tree-structured networks of multiprocessor systems is discussed. A conceptual, knowledge-based operating environment is presented, and requirements for two major technology elements are identified as follows: (1) An intelligent information translator is proposed for implementating information transfer between dissimilar hardware and software, thereby enabling independent and modular development of future systems and promoting a language-independence of codes and information; (2) A resident system activity manager, which recognizes the systems capabilities and monitors the status of all systems within the environment, is proposed for integrating dissimilar systems into effective parallel processing resources to optimally meet user needs. Finally, key computational capabilities which must be provided before the environment can be realized are identified.

  3. Drilling Automation Tests At A Lunar/Mars Analog Site

    Science.gov (United States)

    Glass, B.; Cannon, H.; Hanagud, S.; Lee, P.; Paulsen, G.

    2006-01-01

    Future in-situ lunar/martian resource utilization and characterization, as well as the scientific search for life on Mars, will require access to the subsurface and hence drilling. Drilling on Earth is hard - an art form more than an engineering discipline. The limited mass, energy and manpower in planetary drilling situations makes application of terrestrial drilling techniques problematic. The Drilling Automation for Mars Exploration (DAME) project is developing drilling automation and robotics for projected use in missions to the Moon and Mars in the 2011-15 period. This has been tested recently, drilling in permafrost at a lunar/martian analog site (Haughton Crater, Devon Island, Canada).

  4. Sloan Digital Sky Survey photometric telescope automation and observing software

    International Nuclear Information System (INIS)

    The photometric telescope (PT) provides observations necessary for the photometric calibration of the Sloan Digital Sky Survey (SDSS). Because the attention of the observing staff is occupied by the operation of the 2.5 meter telescope which takes the survey data proper, the PT must reliably take data with little supervision. In this paper we describe the PT's observing program, MOP, which automates most tasks necessary for observing. MOP's automated target selection is closely modeled on the actions a human observer might take, and is built upon a user interface that can be (and has been) used for manual operation. This results in an interface that makes it easy for an observer to track the activities of the automating procedures and intervene with minimum disturbance when necessary. MOP selects targets from the same list of standard star and calibration fields presented to the user, and chooses standard star fields covering ranges of airmass, color, and time necessary to monitor atmospheric extinction and produce a photometric solution. The software determines when additional standard star fields are unnecessary, and selects survey calibration fields according to availability and priority. Other automated features of MOP, such as maintaining the focus and keeping a night log, are also built around still functional manual interfaces, allowing the observer to be as active in observing as desired; MOP's automated features may be used as tools for manual observing, ignored entirely, or allowed to run the telescope with minimal supervision when taking routine data

  5. Open Automated Demand Response Communications Specification (Version 1.0)

    Energy Technology Data Exchange (ETDEWEB)

    Piette, Mary Ann; Ghatikar, Girish; Kiliccote, Sila; Koch, Ed; Hennage, Dan; Palensky, Peter; McParland, Charles

    2009-02-28

    The development of the Open Automated Demand Response Communications Specification, also known as OpenADR or Open Auto-DR, began in 2002 following the California electricity crisis. The work has been carried out by the Demand Response Research Center (DRRC), which is managed by Lawrence Berkeley National Laboratory. This specification describes an open standards-based communications data model designed to facilitate sending and receiving demand response price and reliability signals from a utility or Independent System Operator to electric customers. OpenADR is one element of the Smart Grid information and communications technologies that are being developed to improve optimization between electric supply and demand. The intention of the open automated demand response communications data model is to provide interoperable signals to building and industrial control systems that are preprogrammed to take action based on a demand response signal, enabling a demand response event to be fully automated, with no manual intervention. The OpenADR specification is a flexible infrastructure to facilitate common information exchange between the utility or Independent System Operator and end-use participants. The concept of an open specification is intended to allow anyone to implement the signaling systems, the automation server or the automation clients.

  6. ORIGAMI Automator Primer. Automated ORIGEN Source Terms and Spent Fuel Storage Pool Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Wieselquist, William A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Thompson, Adam B. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Bowman, Stephen M. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Peterson, Joshua L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2016-04-01

    Source terms and spent nuclear fuel (SNF) storage pool decay heat load analyses for operating nuclear power plants require a large number of Oak Ridge Isotope Generation and Depletion (ORIGEN) calculations. SNF source term calculations also require a significant amount of bookkeeping to track quantities such as core and assembly operating histories, spent fuel pool (SFP) residence times, heavy metal masses, and enrichments. The ORIGEN Assembly Isotopics (ORIGAMI) module in the SCALE code system provides a simple scheme for entering these data. However, given the large scope of the analysis, extensive scripting is necessary to convert formats and process data to create thousands of ORIGAMI input files (one per assembly) and to process the results into formats readily usable by follow-on analysis tools. This primer describes a project within the SCALE Fulcrum graphical user interface (GUI) called ORIGAMI Automator that was developed to automate the scripting and bookkeeping in large-scale source term analyses. The ORIGAMI Automator enables the analyst to (1) easily create, view, and edit the reactor site and assembly information, (2) automatically create and run ORIGAMI inputs, and (3) analyze the results from ORIGAMI. ORIGAMI Automator uses the standard ORIGEN binary concentrations files produced by ORIGAMI, with concentrations available at all time points in each assembly’s life. The GUI plots results such as mass, concentration, activity, and decay heat using a powerful new ORIGEN Post-Processing Utility for SCALE (OPUS) GUI component. This document includes a description and user guide for the GUI, a step-by-step tutorial for a simplified scenario, and appendices that document the file structures used.

  7. Analysis of Vitamin D2 and Vitamin D3 in Fortified Milk Powders and Infant and Nutritional Formulas by Liquid Chromatography-Tandem Mass Spectrometry: Single-Laboratory Validation, First Action 2016.05.

    Science.gov (United States)

    Gill, Brendon D; Abernethy, Grant A; Green, Rebecca J; Indyk, Harvey E

    2016-09-01

    A method for the determination of vitamin D2 and vitamin D3 in fortified milk powders and infant and adult nutritional formulas is described. Samples are saponified at high temperature and lipid-soluble components are extracted into isooctane. A portion of the isooctane layer is transferred and washed, and an aliquot of 4-phenyl-1,2,4-triazoline-3,5-dione is added to derivatize the vitamin D to form a high-molecular-mass, easily ionizable adduct. The vitamin D adduct is then re-extracted into a small volume of acetonitrile and analyzed by RPLC. Detection is by tandem MS, using multiple reaction monitoring. Stable isotope-labeled vitamin D2 and vitamin D3 internal standards are used for quantitation to correct for losses in extraction and any variation in derivatization and ionization efficiencies. A single-laboratory validation of the method using AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) kit samples was performed and compared with parameters defined according to the vitamin D Standard Method Performance Requirements (SMPR(®)). Linearity was demonstrated over the range specified in the SMPR, with the LOD being estimated at below that required. Method spike recovery (vitamin D2, 97.0-99.2%; and vitamin D3, 96.0-101.0%) and RSDr (vitamin D3, 1.5-5.2%) were evaluated and compared favorably with limits in the vitamin D SMPR. Acceptable bias for vitamin D3 was demonstrated against both the certified value for National Institute of Standards and Technology 1849a Standard Reference material (P(α = 0.05) = 0.25) and AOAC INTERNATIONAL reference method 2002.05 (P(α = 0.05) = 0.09). The method was demonstrated to meet the requirements of the vitamin D SMPR as defined by SPIFAN, and was recently approved for Official First Action status by the AOAC Expert Review Panel on SPIFAN Nutrient Methods. PMID:27461755

  8. Evidence for social cooperation in rodents by automated maze

    OpenAIRE

    Avi Avital; Shlomit Aga-Mizrachi; Salman Zubedat

    2016-01-01

    Social cooperation is defined as a joint action for mutual benefit that depends on the individual and the counterparts’ behaviors. To gain valid evidence for social cooperation behavior we conducted a series of experiments in our suggested fully automated non-conditioned maze and depicted three major findings: (i) During 18 days of training the rats showed a progressive social learning curve as well as latent social learning; (ii) Examining the perceptual communication between the cooperating...

  9. Automating the radiographic NDT process

    International Nuclear Information System (INIS)

    Automation, the removal of the human element in inspection, has not been generally applied to film radiographic NDT. The justication for automating is not only productivity but also reliability of results. Film remains in the automated system of the future because of its extremely high image content, approximately 8 x 109 bits per 14 x 17. The equivalent to 2200 computer floppy discs. Parts handling systems and robotics applied for manufacturing and some NDT modalities, should now be applied to film radiographic NDT systems. Automatic film handling can be achieved with the daylight NDT film handling system. Automatic film processing is becoming the standard in industry and can be coupled to the daylight system. Robots offer the opportunity to automate fully the exposure step. Finally, computer aided interpretation appears on the horizon. A unit which laser scans a 14 x 17 (inch) film in 6 - 8 seconds can digitize film information for further manipulation and possible automatic interrogations (computer aided interpretation). The system called FDRS (for Film Digital Radiography System) is moving toward 50 micron (*approx* 16 lines/mm) resolution. This is believed to meet the need of the majority of image content needs. We expect the automated system to appear first in parts (modules) as certain operations are automated. The future will see it all come together in an automated film radiographic NDT system (author)

  10. Automating the radiographic ndt process

    International Nuclear Information System (INIS)

    Automation, the removal of the human element in inspection, has not been generally applied to film radiographic NDT. The justification for automating is not only productivity but also reliability of results. Film remains in the automated system of the future because of its extremely high image content, approximately 8 x 109 bits per 14 x 17. This is equivalent to 2200 computer floppy discs. Parts handling systems and robotics applied for manufacturing and some NDT modalities, should now be applied to film radiographic NDT systems. Automatic film handling can be achieved with the daylight NDT film handling system. Automatic film processing is becoming the standard in industry and can be coupled to the daylight system. Robots offer the opportunity to automate fully the exposure step. Finally, computer aided interpretation appears on the horizon. A unit which laser scans a 14 x 17 inch film in 6 - 8 seconds can digitize film information for further manipulation and possible automatic interrogations (computer aided interpretation). The system called FDRS (for Film Digital Radiography System) is moving toward 50 micron (16 lines/mm) resolution. This is believed to meet the need of the majority of image content needs. We expect the automated system to appear first in separate parts (modules) as certain operations are automated. The future will see it all come together in an automated film radiographic NDT system

  11. Fully automated on-line solid phase extraction coupled to liquid chromatography-tandem mass spectrometry for the simultaneous analysis of alkylphenol polyethoxylates and their carboxylic and phenolic metabolites in wastewater samples.

    Science.gov (United States)

    Ciofi, Lorenzo; Ancillotti, Claudia; Chiuminatto, Ugo; Fibbi, Donatella; Pasquini, Benedetta; Bruzzoniti, Maria Concetta; Rivoira, Luca; Del Bubba, Massimo

    2016-05-01

    Three different sorbents (i.e. endcapped octadecylsilane, octasilane and styrene-N-vinylpiperidinone co-polymer) were investigated in order to develop an on-line solid phase extraction-liquid chromatographic tandem mass spectrometric method (on-line SPE-LC-MS/MS) for the simultaneous analysis of alkylphenols polyethoxylate (AP(n)EOs, n = 1-8) and corresponding monocarboxylate (AP1ECs) and phenolic (APs) metabolites. The endcapped octadecylsilane was selected due to its full compatibility with a chromatographic approach, which allowed the elution of positively and negatively ionisable compounds in two distinct retention time windows, using a water-acetonitrile-tetrahydrofuran ternary gradient and a pellicular pentafluorophenyl column. On this SPE sorbent, the composition of the loading/clean-up solution was then optimized in order to achieve the best recoveries of target analytes. Under the best experimental conditions, the total analysis time per sample was 25 min and method detection limits (MDLs) were in the sub-nanograms per litre to nanograms per litre range (0.0081-1.0 ng L(-1)) for AP(n)EOs with n = 2-8, AP1ECs and APs, whereas for AP1EOs, an MDL of about 50 ng L(-1) was found. Using the mass-labelled compound spiking technique, the method performance was tested on inlet and outlet wastewater samples from three activated sludge treatment plants managing domestic and industrial sewages of the urban areas and the textile district of Prato and Bisenzio valley (Tuscany, Italy); in most cases, apparent recovery percentages approximately in the ranges of 50-110% and 80-120% were found for inlet and outlet samples, respectively. The on-line SPE-LC-MS/MS analysis of wastewater samples highlighted the presence of target analytes at concentrations ranging from few nanograms per litre to thousands nanograms per litre, depending on the compound and matrix analysed. AP2ECs were also tentatively identified in outlet samples. PMID:26897380

  12. Automated Fluid Interface System (AFIS)

    Science.gov (United States)

    1990-01-01

    Automated remote fluid servicing will be necessary for future space missions, as future satellites will be designed for on-orbit consumable replenishment. In order to develop an on-orbit remote servicing capability, a standard interface between a tanker and the receiving satellite is needed. The objective of the Automated Fluid Interface System (AFIS) program is to design, fabricate, and functionally demonstrate compliance with all design requirements for an automated fluid interface system. A description and documentation of the Fairchild AFIS design is provided.

  13. Spray automated balancing of rotors - How process parameters influence performance

    Science.gov (United States)

    Smalley, A. J.; Baldwin, R. M.; Fleming, D. P.; Yuhas, J. S.

    1989-01-01

    This paper addresses the application of spray-automated balancing of rotors, and the influence that various operating parameters will have on balancing performance. Spray-automated balancing uses the fuel-air repetitive explosion process to imbed short, discrete bursts of high velocity, high temperature powder into a rotating part at an angle selected to reduce unbalance of the part. The shortness of the burst, the delay in firing of the gun, the speed of the disk and the variability in speed all influence the accuracy and effectiveness of the automated balancing process. The paper evaluates this influence by developing an analytical framework and supplementing the analysis with empirical data obtained while firing the gun at a rotating disk. Encouraging results are obtained, and it is shown that the process should perform satisfactorily over a wide range of operating parameters. Further experimental results demonstrate the ability of the method to reduce vibration levels induced by mass unbalance in a rotating disk.

  14. Automated Quality Assurance of Online NIR Analysers

    Directory of Open Access Journals (Sweden)

    Kari Aaljoki

    2005-01-01

    Full Text Available Modern NIR analysers produce valuable data for closed-loop process control and optimisation practically in real time. Thus it is highly important to keep them in the best possible shape. Quality assurance (QA of NIR analysers is an interesting and complex issue because it is not only the instrument and sample handling that has to be monitored. At the same time, validity of prediction models has to be assured. A system for fully automated QA of NIR analysers is described. The system takes care of collecting and organising spectra from various instruments, relevant laboratory, and process management system (PMS data. Validation of spectra is based on simple diagnostics values derived from the spectra. Predictions are validated against laboratory (LIMS or other online analyser results (collected from PMS. The system features automated alarming, reporting, trending, and charting functions for major key variables for easy visual inspection. Various textual and graphical reports are sent to maintenance people through email. The software was written with Borland Delphi 7 Enterprise. Oracle and PMS ODBC interfaces were used for accessing LIMS and PMS data using appropriate SQL queries. It will be shown that it is possible to take actions even before the quality of predictions is seriously affected, thus maximising the overall uptime of the instrument.

  15. Man-machine interface versus full automation

    International Nuclear Information System (INIS)

    As accelerators grow in size and complexity of operation there is an increasing economical as well as an operational incentive for the controls and operations teams to use computers to help the man-machine interface. At first the computer network replaced the traditional controls racks filled with knobs, buttons and digital displays of voltages and potentiometer readings. The computer system provided the operator with the extension of his hands and eyes. It was quickly found that much more could be achieved. Where previously it was necessary for the human operator to decide the order of the actions to be executed by the computer as a result of a visual indication of malfunctioning of the accelerator, now the operation is becoming more and more under the direct control of the computer system. Expert knowledge is programmed into the system to help the non-specialist make decision and to safeguard the equipment. Machine physics concepts have been incorporated and critical machine parameters can be optimised easily by the physicists or operators without any detailed knowledge of the intervening medium or of the equipment being controlled. As confidence grows and reliability improves, more and more automation can be added. How far can this process of automation replace the skilled operator. Can the accelerators of tomorrow be run like the ever increasing robotic assembly plants of today. How is the role of the operator changing in this new environment

  16. An automated GCxGC-TOF-MS protocol for batch-wise extraction and alignment of mass isotopomer matrixes from differential 13C-labelling experiments: a case study for photoautotrophic-mixotrophic grown Chlamydomonas reinhardtii cells.

    Science.gov (United States)

    Kempa, Stefan; Hummel, Jan; Schwemmer, Thorsten; Pietzke, Matthias; Strehmel, Nadine; Wienkoop, Stefanie; Kopka, Joachim; Weckwerth, Wolfram

    2009-02-01

    Two dimensional gas chromatography coupled to time-of-flight mass spectrometry (GCxGC-TOF-MS) is a promising technique to overcome limits of complex metabolome analysis using one dimensional GC-TOF-MS. Especially at the stage of data export and data mining, however, convenient procedures to cope with the complexity of GCxGC-TOF-MS data are still in development. Here, we present a high sample throughput protocol exploiting first and second retention index for spectral library search and subsequent construction of a high dimensional data matrix useful for statistical analysis. The method was applied to the analysis of (13)C-labelling experiments in the unicellular green alga Chlamydomonas reinhardtii. We developed a rapid sampling and extraction procedure for Chlamydomonas reinhardtii laboratory strain (CC503), a cell wall deficient mutant. By testing all published quenching protocols we observed dramatic metabolite leakage rates for certain metabolites. To circumvent metabolite leakage, samples were directly quenched and analyzed without separation of the medium. The growth medium was adapted to this rapid sampling protocol to avoid interference with GCxGC-TOF-MS analysis. To analyse batches of samples a new software tool, MetMax, was implemented which extracts the isotopomer matrix from stable isotope labelling experiments together with the first and second retention index (RI1 and RI2). To exploit RI1 and RI2 for metabolite identification we used the Golm metabolome database (GMD [1] with RI1/RI2-reference spectra and new search algorithms. Using those techniques we analysed the dynamics of (13)CO(2) and (13)C-acetate uptake in Chlamydomonas reinhardtii cells in two different steady states namely photoautotroph and mixotroph growth conditions. PMID:19206143

  17. Automating usability of ATLAS Distributed Computing resources

    CERN Document Server

    "Tupputi, S A; The ATLAS collaboration

    2013-01-01

    The automation of ATLAS Distributed Computing (ADC) operations is essential to reduce manpower costs and allow performance-enhancing actions, which improve the reliability of the system. In this perspective a crucial case is the automatic exclusion/recovery of ATLAS computing sites storage resources, which are continuously exploited at the edge of their capabilities. It is challenging to adopt unambiguous decision criteria for storage resources who feature non-homogeneous types, sizes and roles. The recently developed Storage Area Automatic Blacklisting (SAAB) tool has provided a suitable solution, by employing an inference algorithm which processes SAM (Site Availability Test) site-by-site SRM tests outcome. SAAB accomplishes both the tasks of providing global monitoring as well as automatic operations on single sites.\

  18. National Automated Conformity Inspection Process

    Data.gov (United States)

    Department of Transportation — The National Automated Conformity Inspection Process (NACIP) Application is intended to expedite the workflow process as it pertains to the FAA Form 81 0-10 Request...

  19. Automation of antimicrobial activity screening.

    Science.gov (United States)

    Forry, Samuel P; Madonna, Megan C; López-Pérez, Daneli; Lin, Nancy J; Pasco, Madeleine D

    2016-03-01

    Manual and automated methods were compared for routine screening of compounds for antimicrobial activity. Automation generally accelerated assays and required less user intervention while producing comparable results. Automated protocols were validated for planktonic, biofilm, and agar cultures of the oral microbe Streptococcus mutans that is commonly associated with tooth decay. Toxicity assays for the known antimicrobial compound cetylpyridinium chloride (CPC) were validated against planktonic, biofilm forming, and 24 h biofilm culture conditions, and several commonly reported toxicity/antimicrobial activity measures were evaluated: the 50 % inhibitory concentration (IC50), the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC). Using automated methods, three halide salts of cetylpyridinium (CPC, CPB, CPI) were rapidly screened with no detectable effect of the counter ion on antimicrobial activity. PMID:26970766

  20. Automating the Purple Crow Lidar

    Science.gov (United States)

    Hicks, Shannon; Sica, R. J.; Argall, P. S.

    2016-06-01

    The Purple Crow LiDAR (PCL) was built to measure short and long term coupling between the lower, middle, and upper atmosphere. The initial component of my MSc. project is to automate two key elements of the PCL: the rotating liquid mercury mirror and the Zaber alignment mirror. In addition to the automation of the Zaber alignment mirror, it is also necessary to describe the mirror's movement and positioning errors. Its properties will then be added into the alignment software. Once the alignment software has been completed, we will compare the new alignment method with the previous manual procedure. This is the first among several projects that will culminate in a fully-automated lidar. Eventually, we will be able to work remotely, thereby increasing the amount of data we collect. This paper will describe the motivation for automation, the methods we propose, preliminary results for the Zaber alignment error analysis, and future work.

  1. Fully automated parallel oligonucleotide synthesizer

    Czech Academy of Sciences Publication Activity Database

    Lebl, M.; Burger, Ch.; Ellman, B.; Heiner, D.; Ibrahim, G.; Jones, A.; Nibbe, M.; Thompson, J.; Mudra, Petr; Pokorný, Vít; Poncar, Pavel; Ženíšek, Karel

    2001-01-01

    Roč. 66, č. 8 (2001), s. 1299-1314. ISSN 0010-0765 Institutional research plan: CEZ:AV0Z4055905 Keywords : automated oligonucleotide synthesizer Subject RIV: CC - Organic Chemistry Impact factor: 0.778, year: 2001

  2. Home automation with Intel Galileo

    CERN Document Server

    Dundar, Onur

    2015-01-01

    This book is for anyone who wants to learn Intel Galileo for home automation and cross-platform software development. No knowledge of programming with Intel Galileo is assumed, but knowledge of the C programming language is essential.

  3. The Automator: Intelligent control system monitoring

    International Nuclear Information System (INIS)

    A large-scale control system may contain several hundred thousand control points which must be monitored to ensure smooth operation. Knowledge of the current state of such a system is often implicit in the values of these points and operators must be cognizant of the state while making decisions. Repetitive operators requiring human intervention lead to fatigue, which can in turn lead to mistakes. The authors propose a tool called the Automator based on a middleware software server. This tool would provide a user-configurable engine for monitoring control points. Based on the status of these control points, a specified action could be taken. The action could range from setting another control point, to triggering an alarm, to running an executable. Often the data presented by a system is meaningless without context information from other channels. Such a tool could be configured to present interpreted information based on values of other channels. Additionally, this tool could translate numerous values in a non-friendly form (such as numbers, bits, or return codes) into meaningful strings of information. Multiple instances of this server could be run, allowing individuals or groups to configure their own Automators. The configuration of the tool will be file-based. In the future, these files could be generated by graphical design tools, allowing for rapid development of new configurations. In addition, the server will be able to explicitly maintain information about the state of the control system. This state information can be used in decision-making processes and shared with other applications. A conceptual framework and software design for the tool are presented

  4. The Automator: Intelligent Control System Monitoring

    International Nuclear Information System (INIS)

    A large-scale control system may contain several hundred thousand control points which must be monitored to ensure smooth operation. Knowledge of the current state of such a system is often implicit in the values of these points and operators must be cognizant of the state while making decisions. Repetitive operators requiring human intervention lead to fatigue, which can in turn lead to mistakes. The authors propose a tool called the Automator based on a middleware software server. This tool would provide a user-configurable engine for monitoring control points. Based on the status of these control points, a specified action could be taken. The action could range from setting another control point, to triggering an alarm, to running an executable. Often the data presented by a system is meaningless without context information from other channels. Such a tool could be configured to present interpreted information based on values of other channels. Additionally, this tool could translate numerous values in a non-friendly form (such as numbers, bits, or return codes) into meaningful strings of information. Multiple instances of this server could be run, allowing individuals or groups to configure their own Automators. The configuration of the tool will be file-based. In the future, these files could be generated by graphical design tools, allowing for rapid development of new configurations. In addition, the server will be able to explicitly maintain information about the state of the control system. This state information can be used in decision-making processes and shared with other applications. A conceptual framework and software design for the tool are presented

  5. Towards automated traceability maintenance.

    Science.gov (United States)

    Mäder, Patrick; Gotel, Orlena

    2012-10-01

    Traceability relations support stakeholders in understanding the dependencies between artifacts created during the development of a software system and thus enable many development-related tasks. To ensure that the anticipated benefits of these tasks can be realized, it is necessary to have an up-to-date set of traceability relations between the established artifacts. This goal requires the creation of traceability relations during the initial development process. Furthermore, the goal also requires the maintenance of traceability relations over time as the software system evolves in order to prevent their decay. In this paper, an approach is discussed that supports the (semi-) automated update of traceability relations between requirements, analysis and design models of software systems expressed in the UML. This is made possible by analyzing change events that have been captured while working within a third-party UML modeling tool. Within the captured flow of events, development activities comprised of several events are recognized. These are matched with predefined rules that direct the update of impacted traceability relations. The overall approach is supported by a prototype tool and empirical results on the effectiveness of tool-supported traceability maintenance are provided. PMID:23471308

  6. Automated document analysis system

    Science.gov (United States)

    Black, Jeffrey D.; Dietzel, Robert; Hartnett, David

    2002-08-01

    A software application has been developed to aid law enforcement and government intelligence gathering organizations in the translation and analysis of foreign language documents with potential intelligence content. The Automated Document Analysis System (ADAS) provides the capability to search (data or text mine) documents in English and the most commonly encountered foreign languages, including Arabic. Hardcopy documents are scanned by a high-speed scanner and are optical character recognized (OCR). Documents obtained in an electronic format bypass the OCR and are copied directly to a working directory. For translation and analysis, the script and the language of the documents are first determined. If the document is not in English, the document is machine translated to English. The documents are searched for keywords and key features in either the native language or translated English. The user can quickly review the document to determine if it has any intelligence content and whether detailed, verbatim human translation is required. The documents and document content are cataloged for potential future analysis. The system allows non-linguists to evaluate foreign language documents and allows for the quick analysis of a large quantity of documents. All document processing can be performed manually or automatically on a single document or a batch of documents.

  7. Automated Supernova Discovery (Abstract)

    Science.gov (United States)

    Post, R. S.

    2015-12-01

    (Abstract only) We are developing a system of robotic telescopes for automatic recognition of Supernovas as well as other transient events in collaboration with the Puckett Supernova Search Team. At the SAS2014 meeting, the discovery program, SNARE, was first described. Since then, it has been continuously improved to handle searches under a wide variety of atmospheric conditions. Currently, two telescopes are used to build a reference library while searching for PSN with a partial library. Since data is taken every night without clouds, we must deal with varying atmospheric and high background illumination from the moon. Software is configured to identify a PSN, reshoot for verification with options to change the run plan to acquire photometric or spectrographic data. The telescopes are 24-inch CDK24, with Alta U230 cameras, one in CA and one in NM. Images and run plans are sent between sites so the CA telescope can search while photometry is done in NM. Our goal is to find bright PSNs with magnitude 17.5 or less which is the limit of our planned spectroscopy. We present results from our first automated PSN discoveries and plans for PSN data acquisition.

  8. Automated Stellar Spectral Classification

    Science.gov (United States)

    Bailer-Jones, Coryn; Irwin, Mike; von Hippel, Ted

    1996-05-01

    Stellar classification has long been a useful tool for probing important astrophysical phenomena. Beyond simply categorizing stars it yields fundamental stellar parameters, acts as a probe of galactic abundance distributions and gives a first foothold on the cosmological distance ladder. The MK system in particular has survived on account of its robustness to changes in the calibrations of the physical parameters. Nonetheless, if stellar classification is to continue as a useful tool in stellar surveys, then it must adapt to keep pace with the large amounts of data which will be acquired as magnitude limits are pushed ever deeper. We are working on a project to automate the multi-parameter classification of visual stellar spectra, using artificial neural networks and other techniques. Our techniques have been developed with 10,000 spectra (B Analysis as a front-end compression of the data. Our continuing work also looks at the application of synthetic spectra to the direct classification of spectra in terms of the physical parameters of Teff, log g, and [Fe/H].

  9. Genetic circuit design automation.

    Science.gov (United States)

    Nielsen, Alec A K; Der, Bryan S; Shin, Jonghyeon; Vaidyanathan, Prashant; Paralanov, Vanya; Strychalski, Elizabeth A; Ross, David; Densmore, Douglas; Voigt, Christopher A

    2016-04-01

    Computation can be performed in living cells by DNA-encoded circuits that process sensory information and control biological functions. Their construction is time-intensive, requiring manual part assembly and balancing of regulator expression. We describe a design environment, Cello, in which a user writes Verilog code that is automatically transformed into a DNA sequence. Algorithms build a circuit diagram, assign and connect gates, and simulate performance. Reliable circuit design requires the insulation of gates from genetic context, so that they function identically when used in different circuits. We used Cello to design 60 circuits forEscherichia coli(880,000 base pairs of DNA), for which each DNA sequence was built as predicted by the software with no additional tuning. Of these, 45 circuits performed correctly in every output state (up to 10 regulators and 55 parts), and across all circuits 92% of the output states functioned as predicted. Design automation simplifies the incorporation of genetic circuits into biotechnology projects that require decision-making, control, sensing, or spatial organization. PMID:27034378

  10. Virtual Machine in Automation Projects

    OpenAIRE

    Xing, Xiaoyuan

    2010-01-01

    Virtual machine, as an engineering tool, has recently been introduced into automation projects in Tetra Pak Processing System AB. The goal of this paper is to examine how to better utilize virtual machine for the automation projects. This paper designs different project scenarios using virtual machine. It analyzes installability, performance and stability of virtual machine from the test results. Technical solutions concerning virtual machine are discussed such as the conversion with physical...

  11. 2015 Chinese Intelligent Automation Conference

    CERN Document Server

    Li, Hongbo

    2015-01-01

    Proceedings of the 2015 Chinese Intelligent Automation Conference presents selected research papers from the CIAC’15, held in Fuzhou, China. The topics include adaptive control, fuzzy control, neural network based control, knowledge based control, hybrid intelligent control, learning control, evolutionary mechanism based control, multi-sensor integration, failure diagnosis, reconfigurable control, etc. Engineers and researchers from academia, industry and the government can gain valuable insights into interdisciplinary solutions in the field of intelligent automation.

  12. Aprendizaje automático

    OpenAIRE

    Moreno, Antonio

    2006-01-01

    En este libro se introducen los conceptos básicos en una de las ramas más estudiadas actualmente dentro de la inteligencia artificial: el aprendizaje automático. Se estudian temas como el aprendizaje inductivo, el razonamiento analógico, el aprendizaje basado en explicaciones, las redes neuronales, los algoritmos genéticos, el razonamiento basado en casos o las aproximaciones teóricas al aprendizaje automático.

  13. Automation of cell line development

    OpenAIRE

    Lindgren, Kristina; Salmén, Andréa; Lundgren, Mats; Bylund, Lovisa; Ebler, Åsa; Fäldt, Eric; Sörvik, Lina; Fenge, Christel; Skoging-Nyberg, Ulrica

    2009-01-01

    An automated platform for development of high producing cell lines for biopharmaceutical production has been established in order to increase throughput and reduce development costs. The concept is based on the Cello robotic system (The Automation Partnership) and covers screening for colonies and expansion of static cultures. In this study, the glutamine synthetase expression system (Lonza Biologics) for production of therapeutic monoclonal antibodies in Chinese hamster ovary cells was used ...

  14. Automating Geographic Information Systems (GIS) through Python for the Hydrological Sciences

    Science.gov (United States)

    Madsen, K.

    2013-12-01

    Geographic Information Systems (GIS) have many applications in the hydrological sciences. However, GIS software is often expensive and difficult to automate. This paper will demonstrate how to automate GRASS GIS software using the Python programming language. Both GRASS GIS and Python are open source projects that are free for anyone to use. Automation of GIS processes is important when dealing with large-scale geographic studies, as large GIS maps are usually divided into discrete tiles. When conducting GIS transformations on such maps, the user must repeat the action for each tile, a process that is greatly expedited through automation. The paper will work through several examples of automated GIS processes and provide complete Python codes that demonstrate correct syntax for working with GRASS GIS applications. The provided examples will demonstrate automation of the following processes 1.) using raster math to calculate foliage thickness from LIDAR and DEM data; 2.) conducting raster interpolation from a set of vector points to develop a continuous hydraulic conductivity coverage; 3.) automating raster coloration to sync the coloration of a large number of raster tiles for website display, and 4.) constructing contoured vector lines from topography rasters. These examples programs will serve as the building blocks for readers, giving them the tools to automate any GIS process using Python and GRASS GIS.

  15. Automated Core Design

    International Nuclear Information System (INIS)

    Multistate searching methods are a subfield of distributed artificial intelligence that aims to provide both principles for construction of complex systems involving multiple states and mechanisms for coordination of independent agents' actions. This paper proposes a multistate searching algorithm with reinforcement learning for the automatic core design of a boiling water reactor. The characteristics of this algorithm are that the coupling structure and the coupling operation suitable for the assigned problem are assumed and an optimal solution is obtained by mutual interference in multistate transitions using multiagents. Calculations in an actual plant confirmed that the proposed algorithm increased the convergence ability of the optimization process

  16. Automated ship image acquisition

    Science.gov (United States)

    Hammond, T. R.

    2008-04-01

    The experimental Automated Ship Image Acquisition System (ASIA) collects high-resolution ship photographs at a shore-based laboratory, with minimal human intervention. The system uses Automatic Identification System (AIS) data to direct a high-resolution SLR digital camera to ship targets and to identify the ships in the resulting photographs. The photo database is then searchable using the rich data fields from AIS, which include the name, type, call sign and various vessel identification numbers. The high-resolution images from ASIA are intended to provide information that can corroborate AIS reports (e.g., extract identification from the name on the hull) or provide information that has been omitted from the AIS reports (e.g., missing or incorrect hull dimensions, cargo, etc). Once assembled into a searchable image database, the images can be used for a wide variety of marine safety and security applications. This paper documents the author's experience with the practicality of composing photographs based on AIS reports alone, describing a number of ways in which this can go wrong, from errors in the AIS reports, to fixed and mobile obstructions and multiple ships in the shot. The frequency with which various errors occurred in automatically-composed photographs collected in Halifax harbour in winter time were determined by manual examination of the images. 45% of the images examined were considered of a quality sufficient to read identification markings, numbers and text off the entire ship. One of the main technical challenges for ASIA lies in automatically differentiating good and bad photographs, so that few bad ones would be shown to human users. Initial attempts at automatic photo rating showed 75% agreement with manual assessments.

  17. AUTOMATED ANALYSIS OF BREAKERS

    Directory of Open Access Journals (Sweden)

    E. M. Farhadzade

    2014-01-01

    Full Text Available Breakers relate to Electric Power Systems’ equipment, the reliability of which influence, to a great extend, on reliability of Power Plants. In particular, the breakers determine structural reliability of switchgear circuit of Power Stations and network substations. Failure in short-circuit switching off by breaker with further failure of reservation unit or system of long-distance protection lead quite often to system emergency.The problem of breakers’ reliability improvement and the reduction of maintenance expenses is becoming ever more urgent in conditions of systematic increasing of maintenance cost and repair expenses of oil circuit and air-break circuit breakers. The main direction of this problem solution is the improvement of diagnostic control methods and organization of on-condition maintenance. But this demands to use a great amount of statistic information about nameplate data of breakers and their operating conditions, about their failures, testing and repairing, advanced developments (software of computer technologies and specific automated information system (AIS.The new AIS with AISV logo was developed at the department: “Reliability of power equipment” of AzRDSI of Energy. The main features of AISV are:· to provide the security and data base accuracy;· to carry out systematic control of breakers conformity with operating conditions;· to make the estimation of individual  reliability’s value and characteristics of its changing for given combination of characteristics variety;· to provide personnel, who is responsible for technical maintenance of breakers, not only with information but also with methodological support, including recommendations for the given problem solving  and advanced methods for its realization.

  18. Automated Production Flow Line Failure Rate Mathematical Analysis with Probability Theory

    Directory of Open Access Journals (Sweden)

    Tan Chan Sin

    2014-12-01

    Full Text Available Automated lines have been widely used in the industries especially for mass production and to customize product. Productivity of automated line is a crucial indicator to show the output and performance of the production. Failure or breakdown of station or mechanisms is commonly occurs in the automated line in real condition due to the technological and technical problem which is highly affect the productivity. The failure rates of automated line are not express or analyse in terms of mathematic form. This paper presents the mathematic analysis by using probability theory towards the failure condition in automated line. The mathematic express for failure rates can produce and forecast the output of productivity accurately

  19. Scaling test of fermion actions in the Schwinger model

    International Nuclear Information System (INIS)

    We discuss the scaling behaviour of different fermion actions in dynamical simulations of the 2-dimensional massive Schwinger model. We have chosen Wilson, hypercube, twisted mass and overlap fermion actions. As physical observables, the pion mass and the scalar condensate are computed for the above mentioned actions at a number of coupling values and fermion masses. We also discuss possibilities to simulate overlap fermions dynamically avoiding problems with low-lying eigenvalues of the overlap kernel

  20. Scaling test of fermion actions in the Schwinger model

    International Nuclear Information System (INIS)

    We discuss the scaling behaviour of different fermion actions in dynamical simulations of the 2-dimensional massive Schwinger model. We have chosen Wilson, hypercube, twisted mass and overlap fermion actions. As physical observables, the pion mass and the scalar condensate are computed for the above mentioned actions at a number of coupling values and fermion masses. We also discuss possibilities to simulate overlap fermions dynamically avoiding problems with low-lying eigenvalues of the overlap kernel. (orig.)

  1. Clinical Chemistry Laboratory Automation in the 21st Century - Amat Victoria curam (Victory loves careful preparation).

    Science.gov (United States)

    Armbruster, David A; Overcash, David R; Reyes, Jaime

    2014-08-01

    The era of automation arrived with the introduction of the AutoAnalyzer using continuous flow analysis and the Robot Chemist that automated the traditional manual analytical steps. Successive generations of stand-alone analysers increased analytical speed, offered the ability to test high volumes of patient specimens, and provided large assay menus. A dichotomy developed, with a group of analysers devoted to performing routine clinical chemistry tests and another group dedicated to performing immunoassays using a variety of methodologies. Development of integrated systems greatly improved the analytical phase of clinical laboratory testing and further automation was developed for pre-analytical procedures, such as sample identification, sorting, and centrifugation, and post-analytical procedures, such as specimen storage and archiving. All phases of testing were ultimately combined in total laboratory automation (TLA) through which all modules involved are physically linked by some kind of track system, moving samples through the process from beginning-to-end. A newer and very powerful, analytical methodology is liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). LC-MS/MS has been automated but a future automation challenge will be to incorporate LC-MS/MS into TLA configurations. Another important facet of automation is informatics, including middleware, which interfaces the analyser software to a laboratory information systems (LIS) and/or hospital information systems (HIS). This software includes control of the overall operation of a TLA configuration and combines analytical results with patient demographic information to provide additional clinically useful information. This review describes automation relevant to clinical chemistry, but it must be recognised that automation applies to other specialties in the laboratory, e.g. haematology, urinalysis, microbiology. It is a given that automation will continue to evolve in the clinical laboratory

  2. Mass Spectrometry in Polymer Chemistry

    CERN Document Server

    Barner-Kowollik, Christopher; Falkenhagen, Jana; Weidner, Steffen

    2011-01-01

    Combining an up-to-date insight into mass-spectrometric polymer analysis beyond MALDI with application details of the instrumentation, this is a balanced and thorough presentation of the most important and widely used mass-spectrometric methods.Written by the world's most proficient experts in the field, the book focuses on the latest developments, covering such technologies and applications as ionization protocols, tandem and liquid chromatography mass spectrometry, gas-phase ion-separation techniques and automated data processing. Chapters on sample preparation, polymer degradation and the u

  3. Programmable automation systems in PSA

    International Nuclear Information System (INIS)

    The Finnish safety authority (STUK) requires plant specific PSAs, and quantitative safety goals are set on different levels. The reliability analysis is more problematic when critical safety functions are realized by applying programmable automation systems. Conventional modeling techniques do not necessarily apply to the analysis of these systems, and the quantification seems to be impossible. However, it is important to analyze contribution of programmable automation systems to the plant safety and PSA is the only method with system analytical view over the safety. This report discusses the applicability of PSA methodology (fault tree analyses, failure modes and effects analyses) in the analysis of programmable automation systems. The problem of how to decompose programmable automation systems for reliability modeling purposes is discussed. In addition to the qualitative analysis and structural reliability modeling issues, the possibility to evaluate failure probabilities of programmable automation systems is considered. One solution to the quantification issue is the use of expert judgements, and the principles to apply expert judgements is discussed in the paper. A framework to apply expert judgements is outlined. Further, the impacts of subjective estimates on the interpretation of PSA results are discussed. (orig.) (13 refs.)

  4. International Conference Automation : Challenges in Automation, Robotics and Measurement Techniques

    CERN Document Server

    Zieliński, Cezary; Kaliczyńska, Małgorzata

    2016-01-01

    This book presents the set of papers accepted for presentation at the International Conference Automation, held in Warsaw, 2-4 March of 2016. It presents the research results presented by top experts in the fields of industrial automation, control, robotics and measurement techniques. Each chapter presents a thorough analysis of a specific technical problem which is usually followed by numerical analysis, simulation, and description of results of implementation of the solution of a real world problem. The presented theoretical results, practical solutions and guidelines will be valuable for both researchers working in the area of engineering sciences and for practitioners solving industrial problems. .

  5. Automated generation of lattice QCD Feynman rules

    Science.gov (United States)

    Hart, A.; von Hippel, G. M.; Horgan, R. R.; Müller, E. H.

    2009-12-01

    The derivation of the Feynman rules for lattice perturbation theory from actions and operators is complicated, especially for highly improved actions such as HISQ. This task is, however, both important and particularly suitable for automation. We describe a suite of software to generate and evaluate Feynman rules for a wide range of lattice field theories with gluons and (relativistic and/or heavy) quarks. Our programs are capable of dealing with actions as complicated as (m)NRQCD and HISQ. Automated differentiation methods are used to calculate also the derivatives of Feynman diagrams. Program summaryProgram title: HiPPY, HPsrc Catalogue identifier: AEDX_v1_0 Program summary URL:http://cpc.cs.qub.ac.uk/summaries/AEDX_v1_0.html Program obtainable from: CPC Program Library, Queen's University, Belfast, N. Ireland Licensing provisions: GPLv2 (see Additional comments below) No. of lines in distributed program, including test data, etc.: 513 426 No. of bytes in distributed program, including test data, etc.: 4 893 707 Distribution format: tar.gz Programming language: Python, Fortran95 Computer: HiPPy: Single-processor workstations. HPsrc: Single-processor workstations and MPI-enabled multi-processor systems Operating system: HiPPy: Any for which Python v2.5.x is available. HPsrc: Any for which a standards-compliant Fortran95 compiler is available Has the code been vectorised or parallelised?: Yes RAM: Problem specific, typically less than 1 GB for either code Classification: 4.4, 11.5 Nature of problem: Derivation and use of perturbative Feynman rules for complicated lattice QCD actions. Solution method: An automated expansion method implemented in Python (HiPPy) and code to use expansions to generate Feynman rules in Fortran95 (HPsrc). Restrictions: No general restrictions. Specific restrictions are discussed in the text. Additional comments: The HiPPy and HPsrc codes are released under the second version of the GNU General Public Licence (GPL v2). Therefore anyone is

  6. WARP (workflow for automated and rapid production): a framework for end-to-end automated digital print workflows

    Science.gov (United States)

    Joshi, Parag

    2006-02-01

    Publishing industry is experiencing a major paradigm shift with the advent of digital publishing technologies. A large number of components in the publishing and print production workflow are transformed in this shift. However, the process as a whole requires a great deal of human intervention for decision making and for resolving exceptions during job execution. Furthermore, a majority of the best-of-breed applications for publishing and print production are intrinsically designed and developed to be driven by humans. Thus, the human-intensive nature of the current prepress process accounts for a very significant amount of the overhead costs in fulfillment of jobs on press. It is a challenge to automate the functionality of applications built with the model of human driven exectution. Another challenge is to orchestrate various components in the publishing and print production pipeline such that they work in a seamless manner to enable the system to perform automatic detection of potential failures and take corrective actions in a proactive manner. Thus, there is a great need for a coherent and unifying workflow architecture that streamlines the process and automates it as a whole in order to create an end-to-end digital automated print production workflow that does not involve any human intervention. This paper describes an architecture and building blocks that lay the foundation for a plurality of automated print production workflows.

  7. Computer automation and artificial intelligence

    International Nuclear Information System (INIS)

    Rapid advances in computing, resulting from micro chip revolution has increased its application manifold particularly for computer automation. Yet the level of automation available, has limited its application to more complex and dynamic systems which require an intelligent computer control. In this paper a review of Artificial intelligence techniques used to augment automation is presented. The current sequential processing approach usually adopted in artificial intelligence has succeeded in emulating the symbolic processing part of intelligence, but the processing power required to get more elusive aspects of intelligence leads towards parallel processing. An overview of parallel processing with emphasis on transputer is also provided. A Fuzzy knowledge based controller for amination drug delivery in muscle relaxant anesthesia on transputer is described. 4 figs. (author)

  8. Manual versus automated blood sampling

    DEFF Research Database (Denmark)

    Teilmann, A C; Kalliokoski, Otto; Sørensen, Dorte B;

    2014-01-01

    corticosterone metabolites, and expressed more anxious behavior than did the mice of the other groups. Plasma corticosterone levels of mice subjected to tail blood sampling were also elevated, although less significantly. Mice subjected to automated blood sampling were less affected with regard to the parameters......Facial vein (cheek blood) and caudal vein (tail blood) phlebotomy are two commonly used techniques for obtaining blood samples from laboratory mice, while automated blood sampling through a permanent catheter is a relatively new technique in mice. The present study compared physiological parameters......, glucocorticoid dynamics as well as the behavior of mice sampled repeatedly for 24 h by cheek blood, tail blood or automated blood sampling from the carotid artery. Mice subjected to cheek blood sampling lost significantly more body weight, had elevated levels of plasma corticosterone, excreted more fecal...

  9. Unmet needs in automated cytogenetics

    International Nuclear Information System (INIS)

    Though some, at least, of the goals of automation systems for analysis of clinical cytogenetic material seem either at hand, like automatic metaphase finding, or at least likely to be met in the near future, like operator-assisted semi-automatic analysis of banded metaphase spreads, important areas of cytogenetic analsis, most importantly the determination of chromosomal aberration frequencies in populations of cells or in samples of cells from people exposed to environmental mutagens, await practical methods of automation. Important as are the clinical diagnostic applications, it is apparent that increasing concern over the clastogenic effects of the multitude of potentially clastogenic chemical and physical agents to which human populations are being increasingly exposed, and the resulting emergence of extensive cytogenetic testing protocols, makes the development of automation not only economically feasible but almost mandatory. The nature of the problems involved, and acutal of possible approaches to their solution, are discussed

  10. Network based automation for SMEs

    DEFF Research Database (Denmark)

    Shahabeddini Parizi, Mohammad; Radziwon, Agnieszka

    2016-01-01

    The implementation of appropriate automation concepts which increase productivity in Small and Medium Sized Enterprises (SMEs) requires a lot of effort, due to their limited resources. Therefore, it is strongly recommended for small firms to open up for the external sources of knowledge, which...... could be obtained through network interaction. Based on two extreme cases of SMEs representing low-tech industry and an in-depth analysis of their manufacturing facilities this paper presents how collaboration between firms embedded in a regional ecosystem could result in implementation of new...... other members of the same regional ecosystem. The findings highlight two main automation related areas where manufacturing SMEs could leverage on external sources on knowledge – these are assistance in defining automation problem as well as appropriate solution and provider selection. Consequently, this...

  11. Design automation for integrated circuits

    Science.gov (United States)

    Newell, S. B.; de Geus, A. J.; Rohrer, R. A.

    1983-04-01

    Consideration is given to the development status of the use of computers in automated integrated circuit design methods, which promise the minimization of both design time and design error incidence. Integrated circuit design encompasses two major tasks: error specification, in which the goal is a logic diagram that accurately represents the desired electronic function, and physical specification, in which the goal is an exact description of the physical locations of all circuit elements and their interconnections on the chip. Design automation not only saves money by reducing design and fabrication time, but also helps the community of systems and logic designers to work more innovatively. Attention is given to established design automation methodologies, programmable logic arrays, and design shortcuts.

  12. Synthesizing individualistic and collectivistic perspectives on environmental and collective action through a relational perspective

    NARCIS (Netherlands)

    van Zomeren, Martijn

    2014-01-01

    Theorizing about environmental or collective action relies on individualistic or collectivistic assumptions, respectively. Thus, a unified explanation for lowering one's thermostat (a form of environmental action) and participating in mass protest (a form of collective action) is lacking. Building o

  13. 程序升温大体积进样和解卷积气相色谱-质谱法测定蔬菜水果中32种农药残留量%Combination of programmable temperature vaporizer-large volume injection gas chromatography-mass spectrometry and automated mass spectral deconvolution and identification system for the determination of 32 pesticides in fruits and vegetables

    Institute of Scientific and Technical Information of China (English)

    曹赵云; 牟仁祥; 吴俐; 林晓燕; 朱智伟; 陈铭学

    2014-01-01

    建立了蔬菜、水果中有机磷、有机氯、拟除虫菊酯和氨基甲酸酯等32种农药的气相色谱-质谱( GC-MS)检测方法。样品经乙腈提取,石墨碳黑串联丙氨基固相萃取柱净化,采用程序升温大体积进样,GC-MS 全扫描模式采集,结合解卷积技术定性分析,内标法定量。分别对程序升温和大体积进样等条件进行了研究,并考察了方法选择性和耐用性。在最优条件下,32种农药的响应值与浓度呈良好的线性关系( r>0.995),各农药的方法检出限为2.0~5.0μg/kg,以菠菜、四季豆和黄瓜为代表基质,进行3个水平(0.010~0.50 mg/kg)的加标回收试验( n=6),回收率为65.2%~120.3%,相对标准偏差( RSD)为4.1%~22.3%。该方法快速、灵敏、可靠、耐用,能满足蔬菜、水果中多类多残留痕量分析的要求。%An analytical method was developed for the simultaneous determination of 32 pesti-cides including organophosphorus, organochlorine, pyrethroid and carbamate pesticides in fruits and vegetables using gas chromatography-mass spectrometry( GC-MS). The sample was extracted with acetonitrile,and the organic layer was cleaned up with ENVI-Carb and LC-NH 2 cartridges. A large volume of 20 μL purified solution was injected into the GC system using pro-grammable temperature vaporizer( PTV). The mass spectrometric detection was operated with full scan mode. The automated mass spectral deconvolution and identfication system( AMDIS) and an isotopic internal standard were used for the qualitative and quantitative determination of the 32 pesticides,respectively. The conditions for PTV-large volume injection were studied. Furthermore,the selectivity and durability of the method were also assessed. Under the opti-mized conditions,the experimental results showed that all the linearities were good within their test ranges,with correlation coefficients more than 0. 995,and the method detection

  14. Automated synthesis of sialylated oligosaccharides

    Directory of Open Access Journals (Sweden)

    Davide Esposito

    2012-09-01

    Full Text Available Sialic acid-containing glycans play a major role in cell-surface interactions with external partners such as cells and viruses. Straightforward access to sialosides is required in order to study their biological functions on a molecular level. Here, automated oligosaccharide synthesis was used to facilitate the preparation of this class of biomolecules. Our strategy relies on novel sialyl α-(2→3 and α-(2→6 galactosyl imidates, which, used in combination with the automated platform, provided rapid access to a small library of conjugation-ready sialosides of biological relevance.

  15. Agile Data: Automating database refactorings

    Directory of Open Access Journals (Sweden)

    Bruno Xavier

    2014-09-01

    Full Text Available This paper discusses an automated approach to database change management throughout the companies’ development workflow. By using automated tools, companies can avoid common issues related to manual database deployments. This work was motivated by analyzing usual problems within organizations, mostly originated from manual interventions that may result in systems disruptions and production incidents. In addition to practices of continuous integration and continuous delivery, the current paper describes a case study in which a suggested pipeline is implemented in order to reduce the deployment times and decrease incidents due to ineffective data controlling.

  16. Automation system for experiment control

    International Nuclear Information System (INIS)

    An automated multi-level system designed for acquisition, accumulation, sorting and processing of information obtained in the course of an experiment is discussed. Intelligent terminals are established at each nuclear installation, which are interconnected with measuring equipment of the corresponding installation. The intelligent terminals operating in the interactive real-time mode permit to use through data links computing facilities and storage of the processing centre. On the top level of the automated system the third generation M4030 computer with 256 Kbyte internal memory is employed. The intelligent terminals are created on the basis of the Ryad-1010 and M-400 computers

  17. Status of automated tensile machine

    International Nuclear Information System (INIS)

    The objective of this work is to develop the Monbusho Automated Tensile machine (MATRON) and install and operate it at the Pacific Northwest Laboratory (PNL). The machine is designed to provide rapid, automated testing of irradiated miniature tensile specimen in a vacuum at elevated temperatures. The MATRON was successfully developed and shipped to PNL for installation in a hot facility. The original installation plan was modified to simplify the current and subsequent installations, and the installation was completed. Detailed procedures governing the operation of the system were written. Testing on irradiated miniature tensile specimen should begin in the near future

  18. Design automation, languages, and simulations

    CERN Document Server

    Chen, Wai-Kai

    2003-01-01

    As the complexity of electronic systems continues to increase, the micro-electronic industry depends upon automation and simulations to adapt quickly to market changes and new technologies. Compiled from chapters contributed to CRC's best-selling VLSI Handbook, this volume covers a broad range of topics relevant to design automation, languages, and simulations. These include a collaborative framework that coordinates distributed design activities through the Internet, an overview of the Verilog hardware description language and its use in a design environment, hardware/software co-design, syst

  19. Automated Podcasting System for Universities

    Directory of Open Access Journals (Sweden)

    Ypatios Grigoriadis

    2013-03-01

    Full Text Available This paper presents the results achieved at Graz University of Technology (TU Graz in the field of automating the process of recording and publishing university lectures in a very new way. It outlines cornerstones of the development and integration of an automated recording system such as the lecture hall setup, the recording hardware and software architecture as well as the development of a text-based search for the final product by method of indexing video podcasts. Furthermore, the paper takes a look at didactical aspects, evaluations done in this context and future outlook.

  20. 2013 Chinese Intelligent Automation Conference

    CERN Document Server

    Deng, Zhidong

    2013-01-01

    Proceedings of the 2013 Chinese Intelligent Automation Conference presents selected research papers from the CIAC’13, held in Yangzhou, China. The topics include e.g. adaptive control, fuzzy control, neural network based control, knowledge based control, hybrid intelligent control, learning control, evolutionary mechanism based control, multi-sensor integration, failure diagnosis, and reconfigurable control. Engineers and researchers from academia, industry, and government can gain an inside view of new solutions combining ideas from multiple disciplines in the field of intelligent automation.   Zengqi Sun and Zhidong Deng are professors at the Department of Computer Science, Tsinghua University, China.

  1. 2013 Chinese Intelligent Automation Conference

    CERN Document Server

    Deng, Zhidong

    2013-01-01

    Proceedings of the 2013 Chinese Intelligent Automation Conference presents selected research papers from the CIAC’13, held in Yangzhou, China. The topics include e.g. adaptive control, fuzzy control, neural network based control, knowledge based control, hybrid intelligent control, learning control, evolutionary mechanism based control, multi-sensor integration, failure diagnosis, and reconfigurable control. Engineers and researchers from academia, industry, and government can gain an inside view of new solutions combining ideas from multiple disciplines in the field of intelligent automation. Zengqi Sun and Zhidong Deng are professors at the Department of Computer Science, Tsinghua University, China.

  2. Toward designing for trust in database automation

    International Nuclear Information System (INIS)

    Appropriate reliance on system automation is imperative for safe and productive work, especially in safety-critical systems. It is unsafe to rely on automation beyond its designed use; conversely, it can be both unproductive and unsafe to manually perform tasks that are better relegated to automated tools. Operator trust in automated tools mediates reliance, and trust appears to affect how operators use technology. As automated agents become more complex, the question of trust in automation is increasingly important. In order to achieve proper use of automation, we must engender an appropriate degree of trust that is sensitive to changes in operating functions and context. In this paper, we present research concerning trust in automation in the domain of automated tools for relational databases. Lee and See have provided models of trust in automation. One model developed by Lee and See identifies three key categories of information about the automation that lie along a continuum of attributional abstraction. Purpose-, process-and performance-related information serve, both individually and through inferences between them, to describe automation in such a way as to engender r properly-calibrated trust. Thus, one can look at information from different levels of attributional abstraction as a general requirements analysis for information key to appropriate trust in automation. The model of information necessary to engender appropriate trust in automation [1] is a general one. Although it describes categories of information, it does not provide insight on how to determine the specific information elements required for a given automated tool. We have applied the Abstraction Hierarchy (AH) to this problem in the domain of relational databases. The AH serves as a formal description of the automation at several levels of abstraction, ranging from a very abstract purpose-oriented description to a more concrete description of the resources involved in the automated process

  3. Automated emergency operating procedures

    International Nuclear Information System (INIS)

    This paper describes the development of a training tool for the symptom oriented emergency operating procedures used at the Laguna Verde Nuclear Power Plant. EOPs and operator training are intended to assist the operator for managing accident situations. A prototype expert system based on the EOPs has been developed for operator training. The demonstration expert system was developed using a commercial shell. The knowledge base consists of two parts. The specific operator actions to be executed for 5 selected accident sequences and the EOPs steps for the reactor pressure vessel control of the water level, pressure, and power. The knowledge is expressed in the form of IF-THEN production rules. A typical training session will display a set of conditions and will prompt the trainee to indicate the appropriate step to perform. This mode will guide the trainee through selected accident sequences. A second mode of the expert system will prompt the trainee for the current plant conditions and the expert system will respond with the EOPs which are required to be performed under these conditions. This allows the trainee to study What if situations

  4. Development of an automated technique for failure modes and effect analysis

    DEFF Research Database (Denmark)

    Blanke, M.; Borch, Ole; Bagnoli, F.;

    Advances in automation have provided integration of monitoring and control functions to enhance the operator's overview and ability to take remedy actions when faults occur. Automation in plant supervision is technically possible with integrated automation systems as platforms, but new design...... methods are needed to cope efficiently with the complexity and to ensure that the functionality of a supervisor is correct and consistent. In particular these methods are expected to significantly improve fault tolerance of the designed systems. The purpose of this work is to develop a software module...... implementing an automated technique for Failure Modes and Effects Analysis (FMEA). This technique is based on the matrix formulation of FMEA for the investigation of failure propagation through a system. As main result, this technique will provide the design engineer with decision tables for fault handling...

  5. Development of an Automated Technique for Failure Modes and Effect Analysis

    DEFF Research Database (Denmark)

    Blanke, M.; Borch, Ole; Allasia, G.;

    1999-01-01

    Advances in automation have provided integration of monitoring and control functions to enhance the operator's overview and ability to take remedy actions when faults occur. Automation in plant supervision is technically possible with integrated automation systems as platforms, but new design...... methods are needed to cope efficiently with the complexity and to ensure that the functionality of a supervisor is correct and consistent. In particular these methods are expected to significantly improve fault tolerance of the designed systems. The purpose of this work is to develop a software module...... implementing an automated technique for Failure Modes and Effects Analysis (FMEA). This technique is based on the matrix formulation of FMEA for the investigation of failure propagation through a system. As main result, this technique will provide the design engineer with decision tables for fault handling...

  6. Monitoring of physics performance of ILC Software based on Higgs Recoil Mass

    Science.gov (United States)

    Volkova, E.; Voutsinas, G.

    2016-02-01

    We discuss a part of ILC software development, that allow us to make automated testing of ILC results. The testing code consists of automated everyday Higgs recoil mass analysis and compares Higgs recoil mass with one of the previous day result. This code uses the result of generation, Mokka simulation and Marlin reconstruction of ILC events.

  7. Automated Traffic Surveillance: Evolution and Implementation

    Directory of Open Access Journals (Sweden)

    Kriti Bhandari

    2013-08-01

    Full Text Available The rampant growth in use of four-wheelers has led to an increase in road congestion at all signal points. In the light of time constraints, drivers find an easier way out by breaking signals. Presently, our defense against such practices lies at the mercy of traffic policemen stationed at various signal points in the city and monitoring of surveillance cameras. However, constant manual monitoring is not always feasible because of limited manpower. Also, while the use of CCTV cameras only aids in monitoring, fine deduction is still looked after by individual traffic authorities. This system of automated surveillance is based on wireless RF transmission and circumvents the need of expensive wired fiber optic cables. The three key elements of this system include (a infrared transmitter and sensor (b a dual state transmitting system (c a control room managing the data base. The motion of a vehicle passing the pre-established line near the signal is judged by the infrared arrangement, including the strip of sensors on the lower surface of the vehicle under consideration and the IR transmitter on the surface of the road, precisely on the above mentioned limiting line at the signal. The presence of the vehicle thus detected will be accounted for by a transmitter installed in it, thus changing its regular state of transmission and providing the unique number of the offender’s vehicle to the control room. The transmission stage works on the RF frequency. FSK modulation being a reliable and apt scheme has been used for the purpose of modulation of data. This brings us to the next stage of the project dealing with the data base matching and there after sending a plan of action to the undersigned bank of the user wherein the fine deduction process will be initiated. Thus in this paper, we focus on simplifying surveillance by introducing automated monitoring system.

  8. Automated Ply Inspection (API) for AFP Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The Automated Ply Inspection (API) system autonomously inspects layups created by high speed automated fiber placement (AFP) machines. API comprises a high accuracy...

  9. Laboratory automation and LIMS in forensics

    DEFF Research Database (Denmark)

    Stangegaard, Michael; Hansen, Anders Johannes; Morling, Niels

    2013-01-01

    Implementation of laboratory automation and LIMS in a forensic laboratory enables the laboratory, to standardize sample processing. Automated liquid handlers can increase throughput and eliminate manual repetitive pipetting operations, known to result in occupational injuries to the technical staff...

  10. Towards Automated System Synthesis Using SCIDUCTION

    OpenAIRE

    Jha, Susmit Kumar

    2011-01-01

    Automated synthesis of systems that are correct by construction has been a long-standing goal of computer science. Synthesis is a creative task and requires human intuition and skill. Its complete automation is currently beyond the capacity of programs that do automated reasoning. However, there is a pressing need for tools and techniques that can automate non-intuitive and error-prone synthesis tasks. This thesis proposes a novel synthesis approach to solve such tasks in the synthesis of pro...

  11. GUI test automation for Qt application

    OpenAIRE

    Wang, Lei

    2015-01-01

    GUI test automation is a popular and interesting subject in the testing industry. Many companies plan to start test automation projects in order to implement efficient, less expensive software testing. However, there are challenges for the testing team who lack experience performing GUI tests automation. Many GUI test automation projects have ended in failure due to mistakes made during the early stages of the project. The major work of this thesis is to find a solution to the challenges of e...

  12. Automated Integrated Analog Filter Design Issues

    OpenAIRE

    Karolis Kiela; Romualdas Navickas

    2015-01-01

    An analysis of modern automated integrated analog circuits design methods and their use in integrated filter design is done. Current modern analog circuits automated tools are based on optimization algorithms and/or new circuit generation methods. Most automated integrated filter design methods are only suited to gmC and switched current filter topologies. Here, an algorithm for an active RC integrated filter design is proposed, that can be used in automated filter designs. The algorithm is t...

  13. Automated activation-analysis system

    International Nuclear Information System (INIS)

    An automated delayed neutron counting and instrumental neutron activation analysis system has been developed at Los Alamos National Laboratory's Omega West Reactor (OWR) to analyze samples for uranium and 31 additional elements with a maximum throughput of 400 samples per day. The system and its mode of operation for a large reconnaissance survey are described

  14. Automated Analysis of Infinite Scenarios

    DEFF Research Database (Denmark)

    Buchholtz, Mikael

    The security of a network protocol crucially relies on the scenario in which the protocol is deployed. This paper describes syntactic constructs for modelling network scenarios and presents an automated analysis tool, which can guarantee that security properties hold in all of the (infinitely many...

  15. Automated visual inspection of textile

    DEFF Research Database (Denmark)

    Jensen, Rune Fisker; Carstensen, Jens Michael

    A method for automated inspection of two types of textile is presented. The goal of the inspection is to determine defects in the textile. A prototype is constructed for simulating the textile production line. At the prototype the images of the textile are acquired by a high speed line scan camera...

  16. Automation of Space Inventory Management

    Science.gov (United States)

    Fink, Patrick W.; Ngo, Phong; Wagner, Raymond; Barton, Richard; Gifford, Kevin

    2009-01-01

    This viewgraph presentation describes the utilization of automated space-based inventory management through handheld RFID readers and BioNet Middleware. The contents include: 1) Space-Based INventory Management; 2) Real-Time RFID Location and Tracking; 3) Surface Acoustic Wave (SAW) RFID; and 4) BioNet Middleware.

  17. Distribution system analysis and automation

    CERN Document Server

    Gers, Juan

    2013-01-01

    A comprehensive guide to techniques that allow engineers to simulate, analyse and optimise power distribution systems which combined with automation, underpin the emerging concept of the "smart grid". This book is supported by theoretical concepts with real-world applications and MATLAB exercises.

  18. Automating the conflict resolution process

    Science.gov (United States)

    Wike, Jeffrey S.

    1991-01-01

    The purpose is to initiate a discussion of how the conflict resolution process at the Network Control Center can be made more efficient. Described here are how resource conflicts are currently resolved as well as the impacts of automating conflict resolution in the ATDRSS era. A variety of conflict resolution strategies are presented.

  19. Automated Clustering of Similar Amendments

    CERN Document Server

    CERN. Geneva

    2016-01-01

    The Italian Senate is clogged by computer-generated amendments. This talk will describe a simple strategy to cluster them in an automated fashion, so that the appropriate Senate procedures can be used to get rid of them in one sweep.

  20. Feasibility Analysis of Crane Automation

    Institute of Scientific and Technical Information of China (English)

    DONG Ming-xiao; MEI Xue-song; JIANG Ge-dong; ZHANG Gui-qing

    2006-01-01

    This paper summarizes the modeling methods, open-loop control and closed-loop control techniques of various forms of cranes, worldwide, and discusses their feasibilities and limitations in engineering. Then the dynamic behaviors of cranes are analyzed. Finally, we propose applied modeling methods and feasible control techniques and demonstrate the feasibilities of crane automation.

  1. Automation; The New Industrial Revolution.

    Science.gov (United States)

    Arnstein, George E.

    Automation is a word that describes the workings of computers and the innovations of automatic transfer machines in the factory. As the hallmark of the new industrial revolution, computers displace workers and create a need for new skills and retraining programs. With improved communication between industry and the educational community to…

  2. Automation of Feynman diagram evaluations

    International Nuclear Information System (INIS)

    A C-program DIANA (DIagram ANAlyser) for the automation of Feynman diagram evaluations is presented. It consists of two parts: the analyzer of diagrams and the interpreter of a special text manipulating language. This language can be used to create a source code for analytical or numerical evaluations and to keep the control of the process in general

  3. Automated methods of corrosion measurement

    DEFF Research Database (Denmark)

    Andersen, Jens Enevold Thaulov; Bech-Nielsen, Gregers; Reeve, John Ch;

    1997-01-01

    to revise assumptions regarding the basis of the method, which sometimes leads to the discovery of as-yet unnoticed phenomena. The present selection of automated methods for corrosion measurements is not motivated simply by the fact that a certain measurement can be performed automatically...

  4. Teacherbot: Interventions in Automated Teaching

    Science.gov (United States)

    Bayne, Sian

    2015-01-01

    Promises of "teacher-light" tuition and of enhanced "efficiency" via the automation of teaching have been with us since the early days of digital education, sometimes embraced by academics and institutions, and sometimes resisted as a set of moves which are damaging to teacher professionalism and to the humanistic values of…

  5. Automation, Labor Productivity and Employment

    DEFF Research Database (Denmark)

    Kromann, Lene; Rose Skaksen, Jan; Sørensen, Anders

    CEBR fremlægger nu den første rapport i AIM-projektet. Rapporten viser, at der er gode muligheder for yderligere automation i en stor del af de danske fremstillingsvirksomheder. For i dag er gennemsnitligt kun omkring 30 % af virksomhedernes produktionsprocesser automatiserede. Navnlig procesområ...

  6. CCD characterization and measurements automation

    Czech Academy of Sciences Publication Activity Database

    Kotov, I.V.; Frank, J.; Kotov, A.I.; Kubánek, Petr; O´Connor, P.; Prouza, Michael; Radeka, V.; Takacs, P.

    2012-01-01

    Roč. 695, Dec (2012), 188-192. ISSN 0168-9002 R&D Projects: GA MŠk ME09052 Institutional research plan: CEZ:AV0Z10100502 Keywords : CCD * characterization * test automation Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 1.142, year: 2012

  7. Adaptation : A Partially Automated Approach

    NARCIS (Netherlands)

    Manjing, Tham; Bukhsh, F.A.; Weigand, H.

    2014-01-01

    This paper showcases the possibility of creating an adaptive auditing system. Adaptation in an audit environment need human intervention at some point. Based on a case study this paper focuses on automation of adaptation process. It is divided into solution design and validation parts. The artifact

  8. Automating the radiographic NDT process

    International Nuclear Information System (INIS)

    Automation, the removal of the human element in inspection has not been generally applied to film radiographic NDT. The justification for automation is not only productivity but also reliability of results. Film remains in the automated system of the future because of its extremely high image content, approximately 3x10 (to the power of nine) bits per 14x17. This is equivalent to 2200 computer floppy disks parts handling systems and robotics applied for manufacturing and some NDT modalities, should now be applied to film radiographic NDT systems. Automatic film handling can be achieved with the daylight NDT film handling system. Automatic film processing is becoming the standard in industry and can be coupled to the daylight system. Robots offer the opportunity to automate fully the exposure step. Finally, a computer aided interpretation appears on the horizon. A unit which laser scans a 14x27 (inch) film in 6-8 seconds can digitize film in information for further manipulation and possible automatic interrogations (computer aided interpretation). The system called FDRS (for film digital radiography system) is moving toward 50 micron (16 lines/mm) resolution. This is believed to meet the need of the majority of image content needs. (Author). 4 refs.; 21 figs

  9. Automating Shallow Seismic Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Steeples, Don W.

    2004-12-09

    This seven-year, shallow-seismic reflection research project had the aim of improving geophysical imaging of possible contaminant flow paths. Thousands of chemically contaminated sites exist in the United States, including at least 3,700 at Department of Energy (DOE) facilities. Imaging technologies such as shallow seismic reflection (SSR) and ground-penetrating radar (GPR) sometimes are capable of identifying geologic conditions that might indicate preferential contaminant-flow paths. Historically, SSR has been used very little at depths shallower than 30 m, and even more rarely at depths of 10 m or less. Conversely, GPR is rarely useful at depths greater than 10 m, especially in areas where clay or other electrically conductive materials are present near the surface. Efforts to image the cone of depression around a pumping well using seismic methods were only partially successful (for complete references of all research results, see the full Final Technical Report, DOE/ER/14826-F), but peripheral results included development of SSR methods for depths shallower than one meter, a depth range that had not been achieved before. Imaging at such shallow depths, however, requires geophone intervals of the order of 10 cm or less, which makes such surveys very expensive in terms of human time and effort. We also showed that SSR and GPR could be used in a complementary fashion to image the same volume of earth at very shallow depths. The primary research focus of the second three-year period of funding was to develop and demonstrate an automated method of conducting two-dimensional (2D) shallow-seismic surveys with the goal of saving time, effort, and money. Tests involving the second generation of the hydraulic geophone-planting device dubbed the ''Autojuggie'' showed that large numbers of geophones can be placed quickly and automatically and can acquire high-quality data, although not under rough topographic conditions. In some easy

  10. Enhanced automation of ECCS at Temelin: the merging of Eastern and Western philosophies

    International Nuclear Information System (INIS)

    After years of separate development, the Eastern and Western concept of safety is now being merged. A significant issue in this process is the philosophy of automation versus operator action for control of safety systems. The paper discusses some aspects of this issue and presents enhancements made at Temelin which merge the Eastern and Western philosophies. (author)

  11. You're a What? Automation Technician

    Science.gov (United States)

    Mullins, John

    2010-01-01

    Many people think of automation as laborsaving technology, but it sure keeps Jim Duffell busy. Defined simply, automation is a technique for making a device run or a process occur with minimal direct human intervention. But the functions and technologies involved in automated manufacturing are complex. Nearly all functions, from orders coming in…

  12. Library Automation : Change for Productivity in Service

    OpenAIRE

    M.A. Gopinath

    1995-01-01

    Library operations, in the context of the automation necessitate for an integrated approach and change in the perception of library's work. It examines the functional aspects, social aspects and system dynamics of library automation. Some strategies for library automation are suggested.

  13. Neutrino masses

    CERN Document Server

    Buccella, F

    2004-01-01

    By requiring the lower limit for the lightest right-handed neutrino mass, obtained in the baryogenesis from leptogenesis scenario, and a Dirac neutrino mass matrix similar to the up-quark mass matrix we predict small values for the $\

  14. Automation for a base station stability testing

    OpenAIRE

    Punnek, Elvis

    2016-01-01

    This Batchelor’s thesis was commissioned by Oy LM Ericsson Ab Oulu. The aim of it was to help to investigate and create a test automation solution for the stability testing of the LTE base station. The main objective was to create a test automation for a predefined test set. This test automation solution had to be created for specific environments and equipment. This work included creating the automation for the test cases and putting them to daily test automation jobs. The key factor...

  15. An Overview of Moonlight Applications Test Automation

    Directory of Open Access Journals (Sweden)

    Appasami Govindasamy

    2010-09-01

    Full Text Available Now-a-days web applications are developed by new technologies like Moonlight, Silverlight, JAVAFX, FLEX, etc. Silverlight is Microsoft's cross platform runtime and development technology for running Web-based multimedia applications in windows platform. Moonlight is an open-source implementation of the Silverlight development platform for Linux and other Unix/X11-based operating systems. It is a new technology in .Net 4.0 to develop rich interactive and attractive platform independent web applications. User Interface Test Automation is very essential for Software industries to reduce test time, cost and man power. Moonlight is new .NET technology to develop rich interactive Internet applications with the collaboration of Novel Corporation. Testing these kinds of applications are not so easy to test, especially the User interface test automation is very difficult. Software test automation has the capability to decrease the overall cost of testing and improve software quality, but most testing organizations have not been able to achieve the full potential of test automation. Many groups that implement test automation programs run into a number of common pitfalls. These problems can lead to test automation plans being completely scrapped, with the tools purchased for test automation becoming expensive. Often teams continue their automation effort, burdened with huge costs in maintaining large suites of automated test scripts. This paper will first discuss some of the key benefits of software test automation, and then examine the most common techniques used to implement software test automation of Moonlight Applications Test Automation. It will then discuss test automation and their potential. Finally, it will do test automation.

  16. Automation of Large-scale Computer Cluster Monitoring Information Analysis

    Science.gov (United States)

    Magradze, Erekle; Nadal, Jordi; Quadt, Arnulf; Kawamura, Gen; Musheghyan, Haykuhi

    2015-12-01

    High-throughput computing platforms consist of a complex infrastructure and provide a number of services apt to failures. To mitigate the impact of failures on the quality of the provided services, a constant monitoring and in time reaction is required, which is impossible without automation of the system administration processes. This paper introduces a way of automation of the process of monitoring information analysis to provide the long and short term predictions of the service response time (SRT) for a mass storage and batch systems and to identify the status of a service at a given time. The approach for the SRT predictions is based on Adaptive Neuro Fuzzy Inference System (ANFIS). An evaluation of the approaches is performed on real monitoring data from the WLCG Tier 2 center GoeGrid. Ten fold cross validation results demonstrate high efficiency of both approaches in comparison to known methods.

  17. Peripheral DSA with automated stepping

    International Nuclear Information System (INIS)

    Peripheral digital angiography can now be applied with automated stepping to evaluate the complete lower extremity, with one single injection of contrast medium. In general, good-quality images have been obtained. In some cases, a stationary run had to be carried out with a digital subtraction angiographic (DSA) technique. The authors report a further improvement of this method: it allows angiographic evaluation of the complete lower extremity with DSA technique after a single contrast medium injection of 60 mL. Altogether, 25 examinations were evaluated and compared with results of the nonsubtracted technique. With the DSA-technique, additional series could be reduced to only those where significant differences in flow between the extremities necessitated different timing, as known from conventional angiography as well. In conclusion, the use of DSA technique with automated stepping may totally replace conventional angiography of the peripheral arteries

  18. Automated nanomanipulation for nanodevice construction

    International Nuclear Information System (INIS)

    Nanowire field-effect transistors (nano-FETs) are nanodevices capable of highly sensitive, label-free sensing of molecules. However, significant variations in sensitivity across devices can result from poor control over device parameters, such as nanowire diameter and the number of electrode-bridging nanowires. This paper presents a fabrication approach that uses wafer-scale nanowire contact printing for throughput and uses automated nanomanipulation for precision control of nanowire number and diameter. The process requires only one photolithography mask. Using nanowire contact printing and post-processing (i.e. nanomanipulation inside a scanning electron microscope), we are able to produce devices all with a single-nanowire and similar diameters at a speed of ∼1 min/device with a success rate of 95% (n = 500). This technology represents a seamless integration of wafer-scale microfabrication and automated nanorobotic manipulation for producing nano-FET sensors with consistent response across devices. (paper)

  19. CCD characterization and measurements automation

    International Nuclear Information System (INIS)

    Modern mosaic cameras have grown both in size and in number of sensors. The required volume of sensor testing and characterization has grown accordingly. For camera projects as large as the LSST, test automation becomes a necessity. A CCD testing and characterization laboratory was built and is in operation for the LSST project. Characterization of LSST study contract sensors has been performed. The characterization process and its automation are discussed, and results are presented. Our system automatically acquires images, populates a database with metadata information, and runs express analysis. This approach is illustrated on 55Fe data analysis. 55Fe data are used to measure gain, charge transfer efficiency and charge diffusion. Examples of express analysis results are presented and discussed.

  20. Automating occupational protection records systems

    International Nuclear Information System (INIS)

    Occupational protection records have traditionally been generated by field and laboratory personnel, assembled into files in the safety office, and eventually stored in a warehouse or other facility. Until recently, these records have been primarily paper copies, often handwritten. Sometimes, the paper is microfilmed for storage. However, electronic records are beginning to replace these traditional methods. The purpose of this paper is to provide guidance for making the transition to automated record keeping and retrieval using modern computer equipment. This paper describes the types of records most readily converted to electronic record keeping and a methodology for implementing an automated record system. The process of conversion is based on a requirements analysis to assess program needs and a high level of user involvement during the development. The importance of indexing the hard copy records for easy retrieval is also discussed. The concept of linkage between related records and its importance relative to reporting, research, and litigation will be addressed. 2 figs

  1. GUI test automation with SWTBot

    OpenAIRE

    Mazurkiewicz, Milosz

    2010-01-01

    In this thesis the author presents theoretical background of GUI test automation as well as technologies, tools and methodologies required to fully understand the test program written in SWTBot. Practical part of the thesis was to implement a program testing File Menu options of Pegasus RCP application developed in Nokia Siemens Networks. Concluding this dissertation, in the author’s opinion test programs written using SWTBot are relatively easy to read and intuitive for people familiar w...

  2. Automated Periodontal Diseases Classification System

    OpenAIRE

    Aliaa A. A. Youssif; Abeer Saad Gawish,; Mohammed Elsaid Moussa

    2012-01-01

    This paper presents an efficient and innovative system for automated classification of periodontal diseases, The strength of our technique lies in the fact that it incorporates knowledge from the patients' clinical data, along with the features automatically extracted from the Haematoxylin and Eosin (H&E) stained microscopic images. Our system uses image processing techniques based on color deconvolution, morphological operations, and watershed transforms for epithelium & connective tissue se...

  3. Automated Scheduling Via Artificial Intelligence

    Science.gov (United States)

    Biefeld, Eric W.; Cooper, Lynne P.

    1991-01-01

    Artificial-intelligence software that automates scheduling developed in Operations Mission Planner (OMP) research project. Software used in both generation of new schedules and modification of existing schedules in view of changes in tasks and/or available resources. Approach based on iterative refinement. Although project focused upon scheduling of operations of scientific instruments and other equipment aboard spacecraft, also applicable to such terrestrial problems as scheduling production in factory.

  4. System of automated map design

    International Nuclear Information System (INIS)

    Preprint 'System of automated map design' contains information about the program shell for construction of territory map, performing level line drawing of arbitrary two-dimension field (in particular, the radionuclide concentration field). The work schedule and data structures are supplied, as well as data on system performance. The preprint can become useful for experts in radioecology and for all persons involved in territory pollution mapping or multi-purpose geochemical mapping. (author)

  5. Magnetic Resonance Connectome Automated Pipeline

    OpenAIRE

    Gray, William R.; Bogovic, John A.; Vogelstein, Joshua T; Landman, Bennett A.; Prince, Jerry L.; Vogelstein, R. Jacob

    2011-01-01

    This manuscript presents a novel, tightly integrated pipeline for estimating a connectome, which is a comprehensive description of the neural circuits in the brain. The pipeline utilizes magnetic resonance imaging (MRI) data to produce a high-level estimate of the structural connectivity in the human brain. The Magnetic Resonance Connectome Automated Pipeline (MRCAP) is efficient and its modular construction allows researchers to modify algorithms to meet their specific requirements. The pipe...

  6. Automation of painted slate inspection

    OpenAIRE

    Carew, Tim

    2002-01-01

    This thesis is concerned with the problem of how to detect visual defects on painted slates using an automated visual inspection system. The vision system that has been developed consists of two major components. The first component addresses issues such as the mechanical implementation and interfacing the inspection system with the optical and sensing equipment whereas the second component involves the development of an image processing algorithm able to identify the visual defects present o...

  7. Automated diagnostics of electronic systems

    OpenAIRE

    Drees, Arto

    2014-01-01

    This thesis was commissioned by Nokia Networks as a part of a wider ongoing quality project. The main objective for this thesis and the quality project was to improve diagnostic accuracy on a certain base station product by targeting the most misdiagnosed faults and to reduce unnecessary component replacement. To achieve the objective, an early version of an automated diagnostic tool was developed. The tool was developed using Microsoft Visual Studio and C# programming language. The tool ...

  8. Automated Analysis of Corpora Callosa

    DEFF Research Database (Denmark)

    Stegmann, Mikkel Bille; Davies, Rhodri H.

    2003-01-01

    This report describes and evaluates the steps needed to perform modern model-based interpretation of the corpus callosum in MRI. The process is discussed from the initial landmark-free contours to full-fledged statistical models based on the Active Appearance Models framework. Topics treated...... include landmark placement, background modelling and multi-resolution analysis. Preliminary quantitative and qualitative validation in a cross-sectional study show that fully automated analysis and segmentation of the corpus callosum are feasible....

  9. Small Business Innovations (Automated Information)

    Science.gov (United States)

    1992-01-01

    Bruce G. Jackson & Associates Document Director is an automated tool that combines word processing and database management technologies to offer the flexibility and convenience of text processing with the linking capability of database management. Originally developed for NASA, it provides a means to collect and manage information associated with requirements development. The software system was used by NASA in the design of the Assured Crew Return Vehicle, as well as by other government and commercial organizations including the Southwest Research Institute.

  10. Method development in automated mineralogy

    OpenAIRE

    Sandmann, Dirk

    2015-01-01

    The underlying research that resulted in this doctoral dissertation was performed at the Division of Economic Geology and Petrology of the Department of Mineralogy, TU Bergakademie Freiberg between 2011 and 2014. It was the primary aim of this thesis to develop and test novel applications for the technology of ‘Automated Mineralogy’ in the field of economic geology and geometallurgy. A “Mineral Liberation Analyser” (MLA) instrument of FEI Company was used to conduct most analytical studies. T...

  11. Efficient And Maintainable Test Automation

    OpenAIRE

    Ahmed, Abdifatah; Lindhe, Magnus

    2002-01-01

    More and more companies experience problems with maintainability and time-consuming development of automated testing tools. The MPC department at Ericsson Software Technology AB use methods and tools often developed during time pressure that results in time-consuming testing and requires more effort and resources than planned. The tools are also such nature that they are hard to expand, maintain and in some cases they have been thrown out between releases. For this reason, we could identify t...

  12. Automated Traffic Enforcement in France

    OpenAIRE

    BRIQUET, Philippe; CANEL, A

    2002-01-01

    One of the goals of traffic safety in cities and between cities is to increase compliance with Traffic Regulations. For this purpose, the French Ministry of Transportation decided to set up an automated traffic enforcement program. This program currently focuses on several types of violations : speeding, spacing between vehicles, and traffic light and railway crossing. This article presents the main goals of the program and the expected challenges and characteristics of operations that have b...

  13. Automated minimax design of networks

    DEFF Research Database (Denmark)

    Madsen, Kaj; Schjær-Jacobsen, Hans; Voldby, J

    1975-01-01

    A new gradient algorithm for the solution of nonlinear minimax problems has been developed. The algorithm is well suited for automated minimax design of networks and it is very simple to use. It compares favorably with recent minimax and leastpth algorithms. General convergence problems related...... to minimax design of networks are discussed. Finally, minimax design of equalization networks for reflectiontype microwave amplifiers is carried out by means of the proposed algorithm....

  14. Automated Planning in Repeated Adversarial Games

    CERN Document Server

    de Cote, Enrique Munoz; Sykulski, Adam M; Jennings, Nicholas R

    2012-01-01

    Game theory's prescriptive power typically relies on full rationality and/or self-play interactions. In contrast, this work sets aside these fundamental premises and focuses instead on heterogeneous autonomous interactions between two or more agents. Specifically, we introduce a new and concise representation for repeated adversarial (constant-sum) games that highlight the necessary features that enable an automated planing agent to reason about how to score above the game's Nash equilibrium, when facing heterogeneous adversaries. To this end, we present TeamUP, a model-based RL algorithm designed for learning and planning such an abstraction. In essence, it is somewhat similar to R-max with a cleverly engineered reward shaping that treats exploration as an adversarial optimization problem. In practice, it attempts to find an ally with which to tacitly collude (in more than two-player games) and then collaborates on a joint plan of actions that can consistently score a high utility in adversarial repeated gam...

  15. Hybrid Action Systems

    DEFF Research Database (Denmark)

    Rönnkö, M.; Ravn, Anders Peter; Sere, K.

    2003-01-01

    In this paper we investigate the use of action systems with differential actions in the specifcation of hybrid systems. As the main contribution we generalize the definition of a differential action, allowing the use of arbitrary relations over model variables and their time-derivatives in modell......In this paper we investigate the use of action systems with differential actions in the specifcation of hybrid systems. As the main contribution we generalize the definition of a differential action, allowing the use of arbitrary relations over model variables and their time...... to the differential action, thus, allowing stepwise development of hybrid systems Udgivelsesdato: JAN 1...

  16. Automated Functional Testing based on the Navigation of Web Applications

    Directory of Open Access Journals (Sweden)

    Boni García

    2011-08-01

    Full Text Available Web applications are becoming more and more complex. Testing such applications is an intricate hard and time-consuming activity. Therefore, testing is often poorly performed or skipped by practitioners. Test automation can help to avoid this situation. Hence, this paper presents a novel approach to perform automated software testing for web applications based on its navigation. On the one hand, web navigation is the process of traversing a web application using a browser. On the other hand, functional requirements are actions that an application must do. Therefore, the evaluation of the correct navigation of web applications results in the assessment of the specified functional requirements. The proposed method to perform the automation is done in four levels: test case generation, test data derivation, test case execution, and test case reporting. This method is driven by three kinds of inputs: i UML models; ii Selenium scripts; iii XML files. We have implemented our approach in an open-source testing framework named Automatic Testing Platform. The validation of this work has been carried out by means of a case study, in which the target is a real invoice management system developed using a model-driven approach.

  17. Statistics in action a Canadian outlook

    CERN Document Server

    Lawless, Jerald F

    2014-01-01

    Commissioned by the Statistical Society of Canada (SSC), Statistics in Action: A Canadian Outlook helps both general readers and users of statistics better appreciate the scope and importance of statistics. It presents the ways in which statistics is used while highlighting key contributions that Canadian statisticians are making to science, technology, business, government, and other areas. The book emphasizes the role and impact of computing in statistical modeling and analysis, including the issues involved with the huge amounts of data being generated by automated processes.The first two c

  18. Axino mass

    CERN Document Server

    Kim, Jihn E

    2012-01-01

    I will talk on my recent works. Axino, related to the SUSY transformation of axion, can mix with Goldstino in principle. In this short talk, I would like to explain what is the axino mass and its plausible mass range. The axino mass is known to have a hierarchical mass structure depending on accidental symmetries. With only one axino, if G_A=0 where G=K+ 2ln|W|, we obtain axino mass= gravitino mass. For G_A nonzero, the axino mass depends on the details of the Kaehler potential. I also comment on the usefulness of a new parametrization of the CKM matrix.

  19. Automated 3D ultrasound image segmentation for assistant diagnosis of breast cancer

    Science.gov (United States)

    Wang, Yuxin; Gu, Peng; Lee, Won-Mean; Roubidoux, Marilyn A.; Du, Sidan; Yuan, Jie; Wang, Xueding; Carson, Paul L.

    2016-04-01

    Segmentation of an ultrasound image into functional tissues is of great importance to clinical diagnosis of breast cancer. However, many studies are found to segment only the mass of interest and not all major tissues. Differences and inconsistencies in ultrasound interpretation call for an automated segmentation method to make results operator-independent. Furthermore, manual segmentation of entire three-dimensional (3D) ultrasound volumes is time-consuming, resource-intensive, and clinically impractical. Here, we propose an automated algorithm to segment 3D ultrasound volumes into three major tissue types: cyst/mass, fatty tissue, and fibro-glandular tissue. To test its efficacy and consistency, the proposed automated method was employed on a database of 21 cases of whole breast ultrasound. Experimental results show that our proposed method not only distinguishes fat and non-fat tissues correctly, but performs well in classifying cyst/mass. Comparison of density assessment between the automated method and manual segmentation demonstrates good consistency with an accuracy of 85.7%. Quantitative comparison of corresponding tissue volumes, which uses overlap ratio, gives an average similarity of 74.54%, consistent with values seen in MRI brain segmentations. Thus, our proposed method exhibits great potential as an automated approach to segment 3D whole breast ultrasound volumes into functionally distinct tissues that may help to correct ultrasound speed of sound aberrations and assist in density based prognosis of breast cancer.

  20. Hybrid Action Systems

    DEFF Research Database (Denmark)

    Ronkko, Mauno; Ravn, Anders P.

    1997-01-01

    a differential action, which allows differential equations as primitive actions. The extension allows us to model hybrid systems with both continuous and discrete behaviour. The main result of this paper is an extension of such a hybrid action system with parallel composition. The extension does not change...... the original meaning of the parallel composition, and therefore also the ordinary action systems can be composed in parallel with the hybrid action systems....