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Sample records for autologous platelet-rich fibrin

  1. Effect of topical autologous platelet-rich fibrin versus no intervention on epithelialization of donor sites and meshed split-thickness skin autografts: a randomized clinical trial

    DEFF Research Database (Denmark)

    Danielsen, P.; Jorgensen, B.; Jorgensen, L.N.;

    2008-01-01

    BACKGROUND: Autologous platelet-rich fibrin contains multiple growth factors. The aim of this randomized clinical trial was to study the effect of topical platelet-rich fibrin on epithelialization of donor sites and meshed split-thickness skin autografts. METHODS: Twenty consecutive leg ulcer...... was not significantly influenced by platelet-rich fibrin treatment Udgivelsesdato: 2008/11...

  2. Examining the effects of platelet-rich plasma and platelet-rich fibrin on autologous full thickness skin graft survival in rats

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    Noorahmad Latifi

    2016-02-01

    Full Text Available Background: Graft survival has been considered the major problem in reconstructive surgery. Clinical studies have helped us to understand the role of PRP in increasing skin survival. Our goal in this study was to examine the treatment effects of platelet-rich plasma (PRP and platelet-rich fibrin (PRF on autologous full thickness skin graft survival in male rats. Methods: This experimental study was performed on 36 rats of Sprague-Dawley race with weighing approximately 250 to 300 gr on May 2015 in animal laboratory of Hazrat Fatima Hospital. After anesthesia, rats were divided into 3 groups. We injected platelet-rich plasma (PRP in the first group, platelet-rich fibrin (PRF in the second and saline in the third group after removing the skin. Microscopic analysis was performed with camera (Canon powershot SX200, Tokyo, Japan on days 7, 14, 21 and 28 after surgery. We used image analysis system (ImageJ, ver. 1.45 to examine necrosis and survival rate. Samples were studied with H&E staining on day 28 microscopically for histological analysis of vascular density and angiogenesis. Results: Our findings showed the area of necrosis in animals injected with PRP on days 7 and 14, was meaningfully less than control group (P= 0.0001. There was no meaningful difference between control and PRP groups (P> 0.05. The area of necrosis in animals injected with PRF did not have any significant difference with control group from beginning to 21st day (P< 0.0001. there was no meaningful difference in vascular density between control and PRP group, whereas in animals injected with PRF the vascular density was significantly less than control group (P= 0.002. Conclusion: According to our results in this study, we can conclude that using autologous PRP can enhance the process of healing soft tissue injury and be affective at increasing graft survival. This method is suggested to be conducted for patients highly at risk of graft loss and also for those who are in need of

  3. Characteristics of an autologous leukocyte and platelet-rich fibrin patch intended for the treatment of recalcitrant wounds

    DEFF Research Database (Denmark)

    Lundquist, Rasmus; Holmstrøm, Kim; Clausen, Christian;

    2012-01-01

    We have investigated the physical, biochemical, and cellular properties of an autologous leukocyte and platelet-rich fibrin patch. This was generated in an automated device from a sample of a patient's blood at the point of care. Using microscopy, cell counting, enzyme-linked immunosorbent assay...... of chronic wound fluid. By comparison with traditional platelet-rich plasma, differences in immune components were found. The relevance of these findings was assessed by showing a mitogenic and migratory effect on cultured human dermal fibroblasts. Further, we showed that fibrocytes, a cell type important...... for acute wound healing, could be grown from the patch. The relevance of these findings in relation to the use of the patch for treating recalcitrant wounds is discussed....

  4. Characteristics of an autologous leukocyte and platelet-rich fibrin patch intended for the treatment of recalcitrant wounds.

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    Lundquist, Rasmus; Holmstrøm, Kim; Clausen, Christian; Jørgensen, Bo; Karlsmark, Tonny

    2013-01-01

    We have investigated the physical, biochemical, and cellular properties of an autologous leukocyte and platelet-rich fibrin patch. This was generated in an automated device from a sample of a patient's blood at the point of care. Using microscopy, cell counting, enzyme-linked immunosorbent assay, antibody arrays, and cell culture assays, we show that the patch is a three-layered membrane comprising a fibrin sheet, a layer of platelets, and a layer of leukocytes. Mean recovery of platelets from the donated blood was 98% (±95%CI 0.8%). Mean levels of platelet-derived growth factor AB, human transforming growth factor beta 1, and vascular endothelial growth factor extracted from the patch were determined as 127 ng (±95% CI 20), 92 ng (±95%CI 17), and 1.35 ng (±95%CI 0.37), respectively. We showed a continued release of PDGF-AB over several days, the rate of which was increased by the addition of chronic wound fluid. By comparison with traditional platelet-rich plasma, differences in immune components were found. The relevance of these findings was assessed by showing a mitogenic and migratory effect on cultured human dermal fibroblasts. Further, we showed that fibrocytes, a cell type important for acute wound healing, could be grown from the patch. The relevance of these findings in relation to the use of the patch for treating recalcitrant wounds is discussed.

  5. Use of an autologous leucocyte and platelet-rich fibrin patch on hard-to-heal DFUs

    DEFF Research Database (Denmark)

    Löndahl, M; Tarnow, L.; Karlsmark, T;

    2015-01-01

    OBJECTIVE: Leucopatch is a leukocyte and platelet-rich fibrin patch that provides concentrated blood cells and signal substances to the surface of an ulcer. It is produced by centrifugation of the patient's own venous blood. The aim of this pilot multicentre cohort study was to evaluate effects o...

  6. A pilot study to evaluate the safety and clinical performance of Leucopatch, an autologous, additive-free, platelet-rich fibrin for the treatment of recalcitrant chronic wounds

    DEFF Research Database (Denmark)

    Jørgensen, Bo; Karlsmark, Tonny; Vogensen, Hanne;

    2011-01-01

    This prospective, uncontrolled pilot study evaluated the safety and clinical performance of Leucopatch an additive-free, autologous platelet-rich fibrin in the treatment of recalcitrant chronic wounds. Fifteen patients, with 16 lower extremity chronic wounds of varying etiologies were treated...... events. Two adverse events, one of noncompliance and one infection, were observed; neither was considered to be related to treatment. The results indicate that Leucopatch is easy to prepare and apply in the clinic, is safe, and may be a clinically effective treatment of recalcitrant chronic wounds....

  7. Platelet rich fibrin in jaw defects

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    Nica, Diana; Ianes, Emilia; Pricop, Marius

    2016-03-01

    Platelet rich fibrin (PRF) is a tissue product of autologous origin abundant in growth factors, widely used in regenerative procedures. Aim of the study: Evaluation of the regenerative effect of PRF added in the bony defects (after tooth removal or after cystectomy) Material and methods: The comparative nonrandomized study included 22 patients divided into 2 groups. The first group (the test group) included 10 patients where the bony defects were treated without any harvesting material. The second group included 12 patients where the bony defects were filled with PRF. The bony defect design was not critical, with one to two walls missing. After the surgeries, a close clinically monitoring was carried out. The selected cases were investigated using both cone beam computer tomography (CBCT) and radiographic techniques after 10 weeks postoperatively. Results: Faster bone regeneration was observed in the bony defects filled with PRF comparing with the not grafted bony defects. Conclusions: PRF added in the bony defects accelerates the bone regeneration. This simplifies the surgical procedures and decreases the economic costs.

  8. Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial.

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    Madurantakam, Parthasarathy; Yoganarasimha, Suyog; Hasan, Fadi K

    2015-09-29

    Autologous platelet concentrates represent promising innovative tools in the field of regenerative medicine and have been extensively used in oral surgery. Unlike platelet rich plasma (PRP) that is a gel or a suspension, Leukocyte-Platelet Rich Fibrin (L-PRF) is a solid 3D fibrin membrane generated chair-side from whole blood containing no anti-coagulant. The membrane has a dense three dimensional fibrin matrix with enriched platelets and abundant growth factors. L-PRF is a popular adjunct in surgeries because of its superior handling characteristics as well as its suturability to the wound bed. The goal of the study is to demonstrate generation as well as provide detailed characterization of relevant properties of L-PRF that underlie its clinical success.

  9. Platelet-Rich Fibrin Promotes an Accelerated Healing of Achilles Tendon When Compared to Platelet-Rich Plasma in Rat

    OpenAIRE

    Dietrich, Franciele; L. Duré, Gustavo; P. Klein, Caroline; F. Bampi, Vinícius; V. Padoin, Alexandre; D. Silva, Vinícius; Braga-Silva, Jefferson

    2015-01-01

    BACKGROUND Autologous platelet concentrate has been used to improve the function and regeneration of injured tissues. Tendinopathies are common in clinical practice, although long-term treatment is required. On the basis of lead time, we compared the effect of using platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in repairing rat Achilles tendon. METHODS The effectiveness of using PRP and PRF was evaluated after 14 and 28 postoperative days by histological analysis. The quantificati...

  10. Platelet-rich fibrin versus albumin in surgical wound repair: a randomized trial with paired design

    DEFF Research Database (Denmark)

    Danielsen, Patricia L; Ågren, Sven Per Magnus; Jørgensen, Lars Nannestad

    2010-01-01

    To study the effects of autologous platelet-rich fibrin (PRF) versus human albumin on incisional wound breaking strength and subcutaneous collagen deposition in patients undergoing laparoscopic cholecystectomy in a randomized trial.......To study the effects of autologous platelet-rich fibrin (PRF) versus human albumin on incisional wound breaking strength and subcutaneous collagen deposition in patients undergoing laparoscopic cholecystectomy in a randomized trial....

  11. A Novel Approach for Treatment of an Unusual Presentation of Radicular Cysts Using Autologous Periosteum and Platelet-Rich Fibrin in Combination with Demineralized Freeze-Dried Bone Allograft

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    Veena A. Patil

    2013-01-01

    Full Text Available Radicular cysts are the most common cystic lesions affecting the jaws. They are most commonly found at the apices of the involved teeth. This condition is usually asymptomatic but can result in a slow-growth tumefaction in the affected region. The following case report presents the successful treatment of radicular cysts using autologous periosteum and platelet-rich fibrin with demineralized freeze-dried bone allograft.

  12. A pilot study to evaluate the safety and clinical performance of Leucopatch, an autologous, additive-free, platelet-rich fibrin for the treatment of recalcitrant chronic wounds

    DEFF Research Database (Denmark)

    Jørgensen, Bo; Karlsmark, Tonny; Vogensen, Hanne;

    2011-01-01

    weekly with Leucopatch, prepared at the point of care from a donation of the patients' blood, for 6 weeks, or until healing was complete. The wounds had been present for 2 to 108 months (median 24 months) and ranged in size from 0.4 to 15.7 cm(2) (median 2.3 cm(2)) and had not responded to previous......This prospective, uncontrolled pilot study evaluated the safety and clinical performance of Leucopatch an additive-free, autologous platelet-rich fibrin in the treatment of recalcitrant chronic wounds. Fifteen patients, with 16 lower extremity chronic wounds of varying etiologies were treated...... treatments. Of the 13 wounds (12 patients) included in the per-protocol efficacy analysis, 4 healed completely (31%). Mean wound area decreased significantly by 65% (95% confidence interval = 45.6% to 83.8%) resulting in a median wound size of 0.9 cm(2) (range = 0-9.6cm(2)). There were no serious adverse...

  13. PLATELET RICH FIBRIN: A PROMISING INNOVATION IN REGENERATIVE THERAPY

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    Arun

    2015-04-01

    Full Text Available Platelets can play a crucial role in regenerative therapy as they are reservoirs of growth factors and cytokines which are the key factors for regeneration of the bone and maturation of the soft tissue. Platelet - rich fibr in (PRF was first described by Choukroun et al. in France. It has been referred to as a second - generation platelet concentrate, which has been shown to have several advantages over traditionally prepared PRP. Platelet - rich fibrin (PRF is autologous plate let concentrates prepared from patient’s own blood. It is a natural fibrin - based biomaterial prepared from an anticoagulant - free blood harvest without any artificial biochemical modification that allows obtaining fibrin membranes enriched with platelets a nd growth factors. Evidence from the literature suggests the potential role of PRF in regeneration and tissue engineering. The slow polymerisation during centrifugation and fibrin - based structure makes PRF a better healing biomaterial than PRP and other fi brin adhesives. The purpose of this review article is to describe the novel second - generation platelet concentrate PRF, which is an improvement over the traditionally prepared PRP for use in regenerative dentistry.

  14. Platelet-rich fibrin matrix for facial plastic surgery.

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    Sclafani, Anthony P; Saman, Masoud

    2012-05-01

    Platelets are known primarily for their role in hemostasis, but there is increasing interest in the effect of platelets on wound healing. Platelet isolates such as platelet-rich plasma have been advocated to enhance and accelerate wound healing. This article describes the use of a novel preparation, platelet-rich fibrin matrix (PRFM), for facial plastic surgery applications such as volume augmentation, fat transfer supplementation, and as an adjunct to open surgical procedures.

  15. Platelet-rich fibrin: Evolution of a second-generation platelet concentrate

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    Sunitha Raja V

    2008-01-01

    Full Text Available Platelet-rich plasma (PRP is a platelet concentrate that has been used widely to accelerate soft-tissue and hard-tissue healing. The preparation of PRP has been described by several authors. Platelet-rich fibrin (PRF was first described by Choukroun et al. in France. It has been referred to as a second-generation platelet concentrate, which has been shown to have several advantages over traditionally prepared PRP. Its chief advantages include ease of preparation and lack of biochemical handling of blood, which makes this preparation strictly autologous. This article describes the evolution of this novel platelet concentrate, referred to as PRF.

  16. One-step apexification using platelet rich fibrin matrix and mineral trioxide aggregate apical barrier

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    Anisha Kumar

    2014-01-01

    In one-step apexification using MTA, the technical problem encountered is controlling the overfill or underfill of MTA. The use of a matrix material helps to overcome this shortcoming. Platelet rich fibrin (PRF is an immune platelet concentrate, which can be used as a matrix, it also promotes wound healing and repair. This case report presents a case of one step apexification using MTA as an apical barrier and autologous PRF as an internal matrix.

  17. Platelet-rich-fibrin: A novel root coverage approach

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    Anilkumar K

    2009-01-01

    Full Text Available Treatment of gingival recession has become an important therapeutic issue due to increasing cosmetic demand. Multiple surgical procedures have been developed to obtain predictable esthetic root coverage. More specifically, after periodontal regenerative surgery, the aim is to achieve complete wound healing and regeneration of the periodontal unit. A recent innovation in dentistry is the preparation and use of platelet-rich plasma (PRP, a concentrated suspension of the growth factors, found in platelets. These growth factors are involved in wound healing and postulated as promoters of tissue regeneration. This paper reports the use of PRF membrane for root coverage on the labial surfaces of the mandibular anterior teeth. This was accomplished using laterally displaced flap technique with platelet rich fibrin (PRF membrane at the recipient site.

  18. Revascularization of Immature Necrotic Teeth: Platelet rich Fibrin an Edge over Platelet rich Plasma

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    Neelam Mittal

    2012-03-01

    Full Text Available Introduction: Revascularization is one such entity that has found its clinical application in the field of endodontics for the manage-ment of immature permanent necrotic teeth. The protocols for revascularization of such teeth focus especially on delivery of stem cells and scaffolds in a nonsurgical manner rather than concentrated growth micro molecules.The hypothesis: This article proposes the role of platelet concentrates such as platelet rich fibrin (PRF and platelet rich plasma (PRP in accelerating the regenerative process in such teeth. PRF unlike PRP is associated with slow, continuous and substantial re-lease of morphogens. It is hypothesized further if PRF instead of PRP when placed through immature apices in an orthograde manner can open newer gates for fast and controlled growth in young, ne-crotic, non-infected teeth.Evaluation of the hypothesis: Enhancement of the healing kinetics can be evaluated by change in size of periapical radiolucency, thickness of the dentinal walls, root elongation and apical closure compared between preoperative and postoperative standardized two dimensional/three dimensional radiographs taken on regular follow ups.

  19. Platelet rich fibrin: a new paradigm in periodontal regeneration.

    Science.gov (United States)

    Kumar, R Vinaya; Shubhashini, N

    2013-09-01

    Among the great challenges facing clinical research is the development of bioactive surgical additives regulating inflammation and increasing healing. Although the use of fibrin adhesives and platelet-rich plasma (PRP) is well documented, they have their own limitations. Hence, reconstructive dental surgeons are looking for an "edge" that jump starts the healing process to maximize predictability as well as the volume of regenerated bone. Overcoming the restrictions related to the reimplantation of blood-derived products, a new family of platelet concentrate, which is neither a fibrin glue nor a classical platelet concentrate, was developed in France. This second generation platelet concentrate called platelet-rich fibrin (PRF), has been widely used to accelerate soft and hard tissue healing. Its advantages over the better known PRP include ease of preparation/application, minimal expense, and lack of biochemical modification (no bovine thrombin or anticoagulant is required). This article serves as an introduction to the PRF "concept" and its potential clinical applications with emphasis on periodontal regeneration.

  20. Revitalization of traumatized immature tooth with platelet-rich fibrin

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    Umrana Faizuddin

    2015-01-01

    Full Text Available Endodontic treatment options for immature, nonvital teeth conventionally include surgical endodontics, apexification with calcium hydroxide, or single visit mineral trioxide aggregate plug. Regeneration is a new concept which is been introduced in the treatment of traumatized open apex tooth. Regeneration of pulp-dentin complex in an infected necrotic tooth with an open apex is possible if the canal is effectively disinfected. The purpose of this case report is to add a new vista in regenerative, endodontic therapy by using platelet-rich fibrin for revitalization of immature nonvital tooth.

  1. Platelet-rich fibrin is a Bioscaffold and reservoir of growth factors for tissue regeneration.

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    Kang, Young-Ho; Jeon, Soung Hoo; Park, Joo-Young; Chung, Jong-Hoon; Choung, Yun-Hoon; Choung, Han-Wool; Kim, Eun-Suk; Choung, Pill-Hoon

    2011-02-01

    The platelet-rich fibrin (PRF) is known as a rich source of autologous cytokines and growth factors and universally used for tissue regeneration in current clinical medicine. However, the microstructure of PRF has not been fully investigated nor have been studied the key molecules that differ PRF from platelet-rich plasma. We fabricated PRF under Choukroun's protocol and produced its extract (PRFe) by freezing at -80°C. The conventional histological, immunohistological staining, and scanning electron microscopy images showed the microstructure of PRF, appearing as two zones, the zone of platelets and the zone of fibrin, which resembled a mesh containing blood cells. The PRFe increased proliferation, migration, and promoted differentiation of the human alveolar bone marrow stem cells (hABMSCs) at 0.5% concentration in vitro. From the results of proteome array, matrix metalloproteinase 9 (MMP9) and Serpin E1 were detected especially in PRFe but not in concentrated platelet-rich plasma. Simultaneous elevation of MMP9, CD44, and transforming growth factor β-1 receptor was shown at 0.5% PRFe treatment to the hABMSC in immunoblot. Mineralization assay showed that MMP9 directly regulated mineralization differentiation of hABMSC. Transplantation of the fresh PRF into the mouse calvarias enhanced regeneration of the critical-sized defect. Our results strongly support the new characteristics of PRF as a bioscaffold and reservoir of growth factors for tissue regeneration.

  2. Effect of Platelet Rich Plasma and Platelet Rich Fibrin on sciatic nerve regeneration in a rat model.

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    Lichtenfels, Martina; Colomé, Lucas; Sebben, Alessandra Deise; Braga-Silva, Jefferson

    2013-07-01

    The aim of this study was to evaluate the effect of Platelet Rich Plasma (PRP) and Platelet Rich Fibrin (PRF) on peripheral nerve repair. Thirty-two Wistar rats were randomly divided into four equal treatments groups: autologous nerve grafts (ANG), silicon tube plus saline solution (SS), silicon tube plus PRP, and silicon tube plus PRF. In ANG group, 10 mm segment from sciatic nerve was excised and reimplanted between the nerve stumps. In the SS, PRP, and PRF groups, 5 mm segment from sciatic nerve was excised and bridged with a 12 mm silicone conduit to create a 10 mm nerve gap. The conduit was filled in accordance with the different treatments. Walking track analysis was performed periodically and on the 90th post-operative day histomorphometric analysis was performed. The ANG, PRF, and PRP groups presented a significant functional improvement in relation to the SS group (P = 0.001) on 90 days after surgery. Histomorphometric analysis demonstrated that the ANG group achieved a larger nerve fiber diameter in proximal stump while comparing with the SS group (P =0.037) and showed larger fiber diameter in median stump in comparison to the PRP group (P = 0.002) and PRF group (P = 0.001). Axonal diameter and myelin sheath thickness showed no statistical significant difference between the groups in the three stumps (P ≥ 0.05). This study suggests that PRP and PRF have positive effects on the functional nerve recovery; however, these groups don't achieve a significant improvement on the histomorphometric analysis.

  3. A recently developed bifacial platelet-rich fibrin matrix

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    E Lucarelli

    2010-07-01

    Full Text Available Platelet-rich plasma (PRP is used clinically in liquid or gel form to promote tissue repair. Because of the poor mechanical properties, conventional PRP is often difficult to handle when used in clinical settings and requires secure implantation in a specific site, otherwise when released growth factors could be washed out during an operation. In this study, we analyzed the end product of a recently developed commercially available system (FIBRINET®, which is a dense pliable, platelet-rich fibrin matrix (PRFM. We characterized the mechanical properties of PRFM and tested whether PRFM releases growth factors and whether released factors induce the proliferation of mesenchymal stem cells (MSC. Mechanical properties as well as platelet distribution were evaluated in PRFM. PRFM demonstrated robust mechanical properties, with a tear elastic modulus of 937.3 + 314.6 kPa, stress at a break of 1476.0 + 526.3 kPa, and an elongation at break of 146.3 + 33.8 %. PRFM maintained its mechanical properties throughout the testing process. Microscopic observations showed that the platelets were localized on one side of the matrix. Elevated levels of PDGF-AA, PDGF-AB, EGF, VEGF, bFGF and TGF-β1 were measured in the day 1-conditioned media (CM of PRFM and growth factor levels decreased thereafter. BMP2 and BMP7 were not detectable. MSC culture media supplemented with 20% PRFM-CM stimulated MSC cell proliferation; at 24 and 48 hours the induction of the proliferation was significantly greater than the induction obtained with media supplemented with 20% foetal bovine serum. The present study shows that the production of a dense, physically robust PRFM made through high-speed centrifugation of intact platelets and fibrin in the absence of exogenous thrombin yields a potential tool for accelerating tissue repair.

  4. A recently developed bifacial platelet-rich fibrin matrix.

    Science.gov (United States)

    Lucarelli, E; Beretta, R; Dozza, B; Tazzari, P L; O'Connel, S M; Ricci, F; Pierini, M; Squarzoni, S; Pagliaro, P P; Oprita, E I; Donati, D

    2010-07-01

    Platelet-rich plasma (PRP) is used clinically in liquid or gel form to promote tissue repair. Because of the poor mechanical properties, conventional PRP is often difficult to handle when used in clinical settings and requires secure implantation in a specific site, otherwise when released growth factors could be washed out during an operation. In this study, we analyzed the end product of a recently developed commercially available system (FIBRINET), which is a dense pliable, platelet-rich fibrin matrix (PRFM). We characterized the mechanical properties of PRFM and tested whether PRFM releases growth factors and whether released factors induce the proliferation of mesenchymal stem cells (MSC). Mechanical properties as well as platelet distribution were evaluated in PRFM. PRFM demonstrated robust mechanical properties, with a tear elastic modulus of 937.3 +/- 314.6 kPa, stress at a break of 1476.0 +/- 526.3 kPa, and an elongation at break of 146.3 +/- 33.8 %. PRFM maintained its mechanical properties throughout the testing process. Microscopic observations showed that the platelets were localized on one side of the matrix. Elevated levels of PDGF-AA, PDGF-AB, EGF, VEGF, bFGF and TGF-beta1 were measured in the day 1-conditioned media (CM) of PRFM and growth factor levels decreased thereafter. BMP2 and BMP7 were not detectable. MSC culture media supplemented with 20% PRFM-CM stimulated MSC cell proliferation; at 24 and 48 hours the induction of the proliferation was significantly greater than the induction obtained with media supplemented with 20% foetal bovine serum. The present study shows that the production of a dense, physically robust PRFM made through high-speed centrifugation of intact platelets and fibrin in the absence of exogenous thrombin yields a potential tool for accelerating tissue repair.

  5. Obtention of injectable platelets rich-fibrin (i-PRF) and its polymerization with bone graft: technical note.

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    Mourão, Carlos Fernando de Almeida Barros; Valiense, Helder; Melo, Elias Rodrigues; Mourão, Natália Belmock Mascarenhas Freitas; Maia, Mônica Diuana-Calasans

    2015-01-01

    The use of autologous platelet concentrates, represent a promising and innovator tools in the medicine and dentistry today. The goal is to accelerate hard and soft tissue healing. Among them, the platelet-rich plasma (PRP) is the main alternative for use in liquid form (injectable). These injectable form of platelet concentrates are often used in regenerative procedures and demonstrate good results. The aim of this study is to present an alternative to these platelet concentrates using the platelet-rich fibrin in liquid form (injectable) and its use with particulated bone graft materials in the polymerized form.

  6. Obtention of injectable platelets rich-fibrin (i-PRF and its polymerization with bone graft: technical note

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    Carlos Fernando de Almeida Barros Mourão

    2015-12-01

    Full Text Available The use of autologous platelet concentrates, represent a promising and innovator tools in the medicine and dentistry today. The goal is to accelerate hard and soft tissue healing. Among them, the platelet-rich plasma (PRP is the main alternative for use in liquid form (injectable. These injectable form ofplatelet concentrates are often used in regenerative procedures and demonstrate good results. The aim of this study is to present an alternative to these platelet concentrates using the platelet-rich fibrin in liquid form (injectable and its use with particulated bone graft materials in the polymerized form.

  7. Role of Platelet rich fibrin in wound healing: A critical review

    Science.gov (United States)

    Naik, Balaram; Karunakar, P; Jayadev, M; Marshal, V Rahul

    2013-01-01

    Aim: The aim is to review and discuss the strategies available for use of platelet rich fibrin as healing aid in dentistry. Background: Platelet rich fibrin (PRF) is a fibrin matrix in which platelet cytokines, growth factors, and cells are trapped and may be released after a certain time and that can serve as a resorbable membrane. Choukroun and his associates were amongst the pioneers for using PRF protocol in oral and maxillofacial surgery to improve bone healing in implant dentistry. Autologous PRF is considered to be a healing biomaterial, and presently, studies have shown its application in various disciplines of dentistry. Materials and Methods: By using specific keywords, electronic search of scientific papers was carried out on the entire PubMed database with custom range of 5 years. The electronic search yielded 302 papers; based on inclusion and exclusion criteria which were specifically predetermined, 72 papers were identified as suitable to the inclusion criteria and the remaining 230 papers were excluded. After adding three more selected papers through hand search, full text of all the articles retrieved and review was done. By pooling the extracted data from selected papers, the reviewed data was synthesized. Conclusion: Recently by showing good promising results with use of the PRF, it has proved to have a good prospect for its use as healing aid in various aspects of the dentistry. PMID:23956527

  8. Bioactivity and stability of endogenous fibrogenic factors in platelet-rich fibrin

    DEFF Research Database (Denmark)

    Lundquist, R.; Dziegiel, M.H.; Agren, M.S.

    2008-01-01

    Platelet-rich fibrin (PRF) is an autologous fibrin sealant (FS) enriched with a platelet concentrate (> 1,000,000 platelets/microL) produced by the automated Vivostat system and used to enhance wound healing. The effects of PRF were compared with supernatant from thrombin-activated platelet...... concentrate, recombinant human platelet-derived growth factor (rhPDGF) isoforms, and a homologous FS in cultured normal human dermal fibroblasts. Also, the release of selected endogenous growth factors from PRF and their stability against proteolytic degradation were studied. The proliferative effect of PRF...... exceeded that of FS and rhPDGF-BB, although it was lower than thrombin-activated platelet concentrate possibly due to sustained growth factor release from platelets in PRF. Anti-PDGF antibody blocked the mitogenic effect of rhPDGF-BB but not that of PRF in growth-arrested fibroblasts. PRF promoted...

  9. Platelet-rich plasma (PRP) and Platelet-Rich Fibrin (PRF): surgical adjuvants, preparations for in situ regenerative medicine and tools for tissue engineering.

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    Bielecki, Tomasz; Dohan Ehrenfest, David M

    2012-06-01

    The recent developement of platelet concentrate for surgical use is an evolution of the fibrin glue technologies used since many years. The initial concept of these autologous preparations was to concentrate platelets and their growth factors in a plasma solution, and to activate it into a fibrin gel on a surgical site, in order to improve local healing. These platelet suspensions were often called Platelet-Rich Plasma (PRP) like the platelet concentrate used in transfusion medicine, but many different technologies have in fact been developed; some of them are even no more platelet suspensions, but solid fibrin-based biomaterials called Platelet-Rich Fibrin (PRF). These various technologies were tested in many different clinical fields, particularly oral and maxillofacial surgery, Ear-Nose-Throat surgery, plastic surgery, orthopaedic surgery, sports medicine, gynecologic and cardiovascular surgery and ophthalmology. This field of research unfortunately suffers from the lack of a proper accurate terminology and the associated misunderstandings, and the literature on the topic is quite contradictory. Indeed, the effects of these preparations cannot be limited to their growth factor content: these products associate many actors of healing in synergy, such as leukocytes, fibrin matrix, and circulating progenitor cells, and are in fact as complex as blood itself. If platelet concentrates were first used as surgical adjuvants for the stimulation of healing (as fibrin glues enriched with growth factors), many applications for in situ regenerative medicine and tissue engineering were developed and offer a great potential. However, the future of this field is first dependent on his coherence and scientific clarity. The objectives of this article is to introduce the main definitions, problematics and perspectives that are described in this special issue of Current Pharmaceutical Biotechnology about platelet concentrates.

  10. Platelet rich fibrin matrix effects on skeletal muscle lesions: an experimental study.

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    Gigante, A; Del Torto, M; Manzotti, S; Cianforlini, M; Busilacchi, A; Davidson, P A; Greco, F; Mattioli-Belmonte, M

    2012-01-01

    Even though muscle injuries are very common, few scientific data on their effective treatment exist. Growth Factors (GFs) may have a role in accelerating muscle repair processes and a currently available strategy for their delivery into the lesion site is the use of autologous platelet-rich plasma (PRP). The present study is focused on the use of Platelet Rich Fibrin Matrix (PRFM), as a source of GFs. Bilateral muscular lesions were created on the longissimus dorsi muscle of Wistar rats. One side of the lesion was filled with a PRFM while the contralateral was left untreated (controls). Animals were sacrificed at 5, 10, 40 and 60 days from surgery. Histological, immunohistochemical and histomorphometric analyses were performed to evaluate muscle regeneration, neovascularization, fibrosis and inflammation. The presence of metaplasia zones, calcifications and heterotopic ossification were also assessed. PRFM treated muscles exhibited an improved muscular regeneration, an increase in neovascularization, and a slight reduction of fibrosis compared with controls. No differences were detected for inflammation. Metaplasia, ossification and heterotopic calcification were not detected. This preliminary morphological experimental study shows that PRFM use can improve muscle regeneration and long-term vascularization. Since autologous blood products are safe, PRFM may be a useful and handy product in clinical treatment of muscle injuries.

  11. Fibrin network architectures in pure platelet-rich plasma as characterized by fiber radius and correlated with clotting time.

    Science.gov (United States)

    Perez, Amanda G M; Rodrigues, Ana A; Luzo, Angela C M; Lana, José F S D; Belangero, William D; Santana, Maria H A

    2014-08-01

    Fibrin networks are obtained through activation of platelet-rich plasma (PRP) for use in tissue regeneration. The importance of fibrin networks relies on mediation of release of growth factors, proliferation of tissue cells and rheological properties of the fibrin gels. Activation of PRP usually involves the decomposition of fibrinogen by agonists, in a wide range of concentrations. Therefore fibrin networks with a large structural diversity are formed, making comparative evaluations difficult. In order to standardize the fibrin networks, we used the statistical techniques central composite rotatable design and response-surface analysis, to correlate the radius of the fibers with the ratios between the agonists (autologous serum/calcium chloride) and agonist/PRP. From an individual and interactive analysis of the variables, architectures characterized by thick, medium and thin fibers were delineated on the response-surface. Furthermore, the architectures were correlated with coagulation time. This approach is valuable for standardizing the PRP preparation for clinical applications.

  12. A recently developed bifacial platelet-rich fibrin matrix

    OpenAIRE

    Lucarelli, E

    2010-01-01

    Platelet-rich plasma (PRP) is used clinically in liquid or gel form to promote tissue repair. Because of the poor mechanical properties, conventional PRP is often difficult to handle when used in clinical settings and requires secure implantation in a specific site, otherwise when released growth factors could be washed out during an operation. In this study, we analyzed the end product of a recently developed commercially available system (FIBRINET®), which is a dense pliable, platelet-rich ...

  13. [The use of platelet concentrates: platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in bone reconstruction prior to dental implant surgery].

    Science.gov (United States)

    Baeyens, W; Glineur, R; Evrard, L

    2010-01-01

    The autologous platelet concentrates--Platelet-Rich Plasma (PRP) and Platelet-Rich Fibrin (PRF)--are used in various medical fields, particularly in oral and maxillofacial surgery. These concentrates contain high levels of growth factors, including the 3 isomers of PDGF (platelet-derived growth factor), 2 of the numerous transforming growth factors (TGF-beta), the insulinlike growth factor (IGF), the epithelial growth factor (EGF) and the vascular endothelial growth factor (VEGF), which are the key elements in wound healing, particularly in bone regeneration. Platelet concentrates are easy to apply in clinical practice and offer potential benefits including rapid wound healing and bone regeneration, and can therefore be considered to be new therapeutic adjuvants. In dental implant surgery they are used in bone reconstruction prior or concomitant to implant procedures, and also for dental extraction socket preservation. Their use result in enhanced bone graft density and maturation. A literature review on the use of PRP/PRF in maxillofacial and dental implant surgery is proposed.

  14. Growth factor and proteinase profile of Vivostat® platelet-rich fibrin linked to tissue repair

    DEFF Research Database (Denmark)

    Ågren, Sven Per Magnus; Rasmussen, Karina; Pakkenberg, Bente;

    2014-01-01

    BACKGROUND AND OBJECTIVES: Autologous platelet-rich fibrin (PRF(®)) is prepared by the automatic Vivostat(®) system. Conflicting results with Vivostat PRF in acute wound healing prompted us to examine its cellular and biomolecular composition. Specifically, platelets, selected growth factors...... and matrix metalloproteinase (MMP)-9 were quantified using novel analytical methods. MATERIALS AND METHODS: Ten healthy non-thrombocytopenic volunteers donated blood for generation of intermediate fibrin-I and final PRF. Anticoagulated whole blood and serum procured in parallel served as baseline controls....... Leucocyte, erythrocyte and platelet counts in whole blood and fibrin-I were determined by automated haematology analyser. Platelet concentration in PRF was quantified manually by stereologic analysis of Giemsa-stained tissue sections, and the total content of five growth factors and MMP-9 by enzyme...

  15. Autologous conditioned serum and platelet-rich plasma in equine orthopedic therapeutics

    OpenAIRE

    Cynthia Prado Vendruscolo; Ana Liz Garcia Alves; Patrícia Monaco Brossi; Raquel Yvonne Arantes Baccarin

    2014-01-01

    Musculoskeletal injuries that occur in horses during sports activities are often disabling and require a long period of treatment and rehabilitation, most resulting in scar tissue, predisposing to recurrence. In search of more effective therapies and tissue regeneration, studies have been carried out with blood derivatives - platelet rich plasma and autologous conditioned serum. In spite of both being bloodderived therapies, platelet rich plasma and autologous conditioned serum are distinct p...

  16. Application of platelet-rich plasma and platelet-rich fibrin in fat grafting: basic science and literature review.

    Science.gov (United States)

    Liao, Han-Tsung; Marra, Kacey G; Rubin, J Peter

    2014-08-01

    Due to the natural properties of fat, fat grafting remains a popular procedure for soft tissue volume augmentation and reconstruction. However, clinical outcome varies and is technique dependent. Platelet-rich plasma (PRP) contains α-granules, from which multiple growth factors such as platelet-derived growth factor, transforming growth factor-β, vascular endothelial growth factor, and epidermal growth factor can be released after activation. In recent years, the scope of PRP therapies has extended from bone regeneration, wound healing, and healing of musculoskeletal injuries, to enhancement of fat graft survival. In this review, we focus on the definition of PRP, the different PRP preparation and activation methods, and growth factor concentrations. In addition, we discuss possible mechanisms for the role of PRP in fat grafting by reviewing in vitro studies with adipose-derived stem cells, preadipocytes, and adipocytes, and preclinical and clinical research. We also review platelet-rich fibrin, a so-called second generation PRP, and its slow-releasing biology and effects on fat grafts compared to PRP in both animal and clinical research. Finally, we provide a general foundation on which to critically evaluate earlier studies, discuss the limitations of previous research, and direct plans for future experiments to improve the optimal effects of PRP in fat grafting.

  17. Treatment of experimental furcation perforations with mineral trioxide aggregate, platelet rich plasma or platelet rich fibrin in dogs' teeth.

    Science.gov (United States)

    Tawfik, Hosam E; Abu-Seida, Ashraf M; Hashem, Ahmed A; El-Khawlani, Mohammed M

    2016-06-01

    This work evaluates the effect of mineral trioxide aggregate (MTA), platelet rich plasma (PRP) or platelet rich fibrin (PRF) on healing of non-contaminated and contaminated furcation perforations. A total of 192 teeth of 12 dogs was divided into three equal groups according to evaluation period. Each group was further subdivided into MTA, PRP, PRF, negative and positive control subgroups. Each experimental subgroup was further subdivided according to perforation status into non-contaminated and contaminated subdivisions. Root canal therapy was carried out and furcation perforation was made in all teeth except in negative control subgroup. The furcation perforation was repaired immediately in subdivision (1) and after 4 weeks in subdivision (2). The change in vertical bone loss was measured by radiography. Inflammatory cell count, cemental deposition, new bone formation, bone resorption and epithelial proliferation were assessed. Both PRP and PRF demonstrated statistically significant reduction in vertical bone loss and inflammatory cell count than MTA. No significant difference was found between MTA, PRP and PRF in cemental deposition, new bone formation, bone resorption and epithelial proliferation. The non-contaminated teeth demonstrated better treatment outcomes than the contaminated teeth. In conclusion, PRP and PRF are successful treatment options for repairing of furcation perforation in both non-contaminated and contaminated teeth in dogs with superior outcomes in non contaminated teeth.

  18. Autologous conditioned serum and platelet-rich plasma in equine orthopedic therapeutics

    Directory of Open Access Journals (Sweden)

    Cynthia Prado Vendruscolo

    2014-10-01

    Full Text Available Musculoskeletal injuries that occur in horses during sports activities are often disabling and require a long period of treatment and rehabilitation, most resulting in scar tissue, predisposing to recurrence. In search of more effective therapies and tissue regeneration, studies have been carried out with blood derivatives - platelet rich plasma and autologous conditioned serum. In spite of both being bloodderived therapies, platelet rich plasma and autologous conditioned serum are distinct products, with equally distinct indications for their use. Platelet rich plasma shows promising results in ligament and tendon injuries in clinical and experimental trials. This occurs also in osteoarticular lesions with both hemoderivates, autologous conditioned serum and platelet rich plasma. This review aims to present clinical and experimental studies (in vivo and in vitro in the equine species, as an aid for an appropriate therapeutic choice, when hemoderivates are considered for treatment of musculoskeletal lesions.

  19. Platelet-rich fibrin membranes as scaffolds for periosteal tissue engineering.

    NARCIS (Netherlands)

    Gassling, V.; Douglas, T.E.L.; Warnke, P.H.; Acil, Y.; Wiltfang, J.; Becker, S.T.

    2010-01-01

    OBJECTIVES: Platelet-rich fibrin (PRF)-based membranes have been used for covering alveolar ridge augmentation side in several in vivo studies. Few in vitro studies on PRF and no studies using human periosteal cells for tissue engineering have been published. The aim is a comparison of PRF with the

  20. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF).

    Science.gov (United States)

    Dohan Ehrenfest, David M; Rasmusson, Lars; Albrektsson, Tomas

    2009-03-01

    The topical use of platelet concentrates is recent and its efficiency remains controversial. Several techniques for platelet concentrates are available; however, their applications have been confusing because each method leads to a different product with different biology and potential uses. Here, we present classification of the different platelet concentrates into four categories, depending on their leucocyte and fibrin content: pure platelet-rich plasma (P-PRP), such as cell separator PRP, Vivostat PRF or Anitua's PRGF; leucocyte- and platelet-rich plasma (L-PRP), such as Curasan, Regen, Plateltex, SmartPReP, PCCS, Magellan or GPS PRP; pure plaletet-rich fibrin (P-PRF), such as Fibrinet; and leucocyte- and platelet-rich fibrin (L-PRF), such as Choukroun's PRF. This classification should help to elucidate successes and failures that have occurred so far, as well as providing an objective approach for the further development of these techniques.

  1. In vivo evaluation of titanium-prepared platelet-rich fibrin (T-PRF): a new platelet concentrate.

    Science.gov (United States)

    Tunalı, Mustafa; Özdemir, Hakan; Küçükodacı, Zafer; Akman, Serhan; Fıratlı, Erhan

    2013-07-01

    We have developed a new, titanium-prepared, platelet-rich fibrin (T-PRF) together with the protocol for forming it, which is based on the hypothesis that titanium tubes may be more effective at activating platelets than the glass tubes used by Chouckroun in his platelet-rich fibrin (PRF) method. The aim of this study was to find a suitable animal model in which to evaluate the method and to investigate the efficacy of T-PRF for wound healing. Blood samples from 6 rabbits were used to confirm the protocol for formation of T-PRF. We evaluated T-PRF or T-PRF-like clots morphologically using scanning electron microscopy (EM). Blood samples from 5 rabbits were used to develop an experiment in which to evaluate the effects of T-PRF on wound healing. The mucoperiosteal flaps were filled with autologous T-PRF membranes from the vestibule in the anterior mandibular regions. Samples collected from the surgical sites were stained with haematoxylin and eosin. We found a mature fibrin network in T-PRF clots that had been centrifuged for 15 min at 3500 rpm and, 15 days after placement of the membrane, we found newly-forming connective tissue and islets of bony tissue in the T-PRF membrane. These results show that T-PRF could induce the formation of new bone with new connective tissue in a rabbit model of wound healing within 30 days of treatment.

  2. A Novel Technique for Conjunctivoplasty in a Rabbit Model: Platelet-Rich Fibrin Membrane Grafting

    Directory of Open Access Journals (Sweden)

    Mehmet Erol Can

    2016-01-01

    Full Text Available Purpose. To investigate the effect of platelet-rich fibrin (PRF membrane on wound healing. Methods. Twenty-four right eyes of 24 New Zealand rabbits equally divided into 2 groups for the study design. After the creation of 5 × 5 mm conjunctival damage, it was secured with PRF membrane, which was generated from the rabbit’s whole blood samples in PRF membrane group, whereas damage was left unsutured in the control group. Three animals were sacrificed in each group on the 1st, 3rd, 7th, and 28th postoperative days. Immunohistochemical (IHC stainings and biomicroscopic evaluation were performed and compared between groups. Results. PRF membrane generated significant expressions of vascular endothelial growth factor (VEGF, transforming growth factor-beta (TGF-β, and platelet-derived growth factor (PDGF in the early postoperative period. However, the IHC evaluation allowed showing the excessive staining at day 28, in control group. Biomicroscopic evaluation revealed complete epithelialization in PRF membrane group, but none of the cases showed complete healing in the control group. Conclusions. This experimental study showed us the beneficial effects of the PRF membrane on conjunctival healing. Besides its chemical effects, it provides mechanical support as a scaffold for the migrating cells that are important for ocular surface regeneration. These overall results encourage us to apply autologous PRF membrane as a growth factor-enriched endogenous scaffold for ocular surface reconstruction.

  3. A Novel Technique for Conjunctivoplasty in a Rabbit Model: Platelet-Rich Fibrin Membrane Grafting

    Science.gov (United States)

    Çakmak, Hasan Basri; Dereli Can, Gamze; Ünverdi, Hatice; Toklu, Yasin; Hücemenoğlu, Sema

    2016-01-01

    Purpose. To investigate the effect of platelet-rich fibrin (PRF) membrane on wound healing. Methods. Twenty-four right eyes of 24 New Zealand rabbits equally divided into 2 groups for the study design. After the creation of 5 × 5 mm conjunctival damage, it was secured with PRF membrane, which was generated from the rabbit's whole blood samples in PRF membrane group, whereas damage was left unsutured in the control group. Three animals were sacrificed in each group on the 1st, 3rd, 7th, and 28th postoperative days. Immunohistochemical (IHC) stainings and biomicroscopic evaluation were performed and compared between groups. Results. PRF membrane generated significant expressions of vascular endothelial growth factor (VEGF), transforming growth factor-beta (TGF-β), and platelet-derived growth factor (PDGF) in the early postoperative period. However, the IHC evaluation allowed showing the excessive staining at day 28, in control group. Biomicroscopic evaluation revealed complete epithelialization in PRF membrane group, but none of the cases showed complete healing in the control group. Conclusions. This experimental study showed us the beneficial effects of the PRF membrane on conjunctival healing. Besides its chemical effects, it provides mechanical support as a scaffold for the migrating cells that are important for ocular surface regeneration. These overall results encourage us to apply autologous PRF membrane as a growth factor-enriched endogenous scaffold for ocular surface reconstruction. PMID:27747098

  4. Effect of platelet-rich plasma and platelet-rich fibrin on peri-implant bone defects in dogs.

    Science.gov (United States)

    Jeong, Kyung-In; Kim, Su-Gwan; Oh, Ji-Su; Lee, Sung-Yong; Cho, Young-Seung; Yang, Sung-Soo; Park, Seung-Cheol; You, Jae-Seek; Lim, Sung-Chul; Jeong, Mi-Ae; Kim, Jae-Sung; Lee, Sook-Young

    2013-03-01

    The purpose of this study was to evaluate the effect of tooth ash and platelet-rich plasma (PRP), and platelet-rich fibrin (PRF) grafts into bone defects around implants on bone formation. Six adult dogs were used as experimental subjects. Graft materials were used to create a particulate material. Forty-eight tapered-type implants, 3.7 mm in diameter, 10 mm in length, and with surface treated with hydroxyapatite (HA) coating, were used as implant fixtures. Using a trephine bur, four bone defects were formed and implants were placed in the femurs of the adult dogs. Bone grafts were not performed in the control group. Tooth ash was grafted into the defects in group 1. In group 2, a mixture of tooth ash and PRP (1:1 ratio by volume) was grafted into the defects. In group 3, a mixture of tooth ash and PRF (ratio of 1:1) was grafted in the defect area. Animals were sacrificed after 4 or 8 weeks. Based on histopathological examination, the amount and rate of new bone formation were evaluated. Histomorphometric examination revealed that the rate of new bone formation in group 3 of the 4-week group was significantly higher than that in the control group. In addition, in the 8-week group, a significant increase in new bone formation was confirmed in group 3. In this study, a bone graft method using a mixture of tooth ash and PRF was found to increase new bone formation compared to the method using PRP. In addition, it was confirmed that this effect was more prominent in the initial stage of the bone graft.

  5. Platelet-rich fibrin-induced bone marrow mesenchymal stem cell differentiation into osteoblast-like cells and neural cells

    Institute of Scientific and Technical Information of China (English)

    Qi Li; Yajun Geng; Lei Lu; Tingting Yang; Mingrui Zhang; Yanmin Zhou

    2011-01-01

    Bone marrow mesenchymal stem cells were allowed to develop for 14 days in a platelet-rich fibrin environment. Results demonstrated that platelet-rich fibrin significantly promoted bone marrow mesenchymal stem cell proliferation. In addition, there was a dose-dependent increase in Runt-related transcription factor-2 and bone morphogenetic protein-2 mRNA expression, as well as neuron-specific enolase and glial acidic protein. Results showed that platelet-rich fibrin promoted bone marrow mesenchymal stem cell proliferation and differentiation of osteoblastlike cells and neural cells in a dose-dependent manner.

  6. Platelet Rich Fibrin Matrix Improves Wound Angiogenesis via Inducing Endothelial Cell Proliferation

    OpenAIRE

    Roy, Sashwati; Driggs, Jason; Elgharably, Haytham; Biswas, Sabyasachi; Findley, Muna; Khanna, Savita; Gnyawali, Urmila; Bergdall, Valerie K.; Sen, Chandan K.

    2011-01-01

    The economic, social and public health burden of chronic ulcers and other compromised wounds are enormous and rapidly increasing with the aging population. The growth factors derived from platelets play an important role in tissue remodeling including neovascularization. Platelet-rich plasma (PRP) has been utilized and studied for the last four decades. Platelet gel and fibrin sealant, derived from PRP mixed with thrombin and calcium chloride, have been exogenously applied to tissues to promo...

  7. Magnesium-enhanced enzymatically mineralized platelet-rich fibrin for bone regeneration applications.

    Science.gov (United States)

    Gassling, Volker; Douglas, Timothy E L; Purcz, Nicolai; Schaubroeck, David; Balcaen, Lieve; Bliznuk, Vitaliy; Declercq, Heidi A; Vanhaecke, Frank; Dubruel, Peter

    2013-10-01

    Membranes of the autologous blood-derived biomaterial platelet-rich fibrin (PRF) were mineralized enzymatically with calcium phosphate (CaP) by the incorporation of alkaline phosphatase (ALP) followed by incubation for 3 days in solutions of either 0.1 M calcium glycerophosphate (CaGP) or a combination of CaGP and magnesium glycerophosphate (CaGP:MgGP; both 0.05 M), resulting in the formation of two different PRF-mineral composites. Fourier transform infrared spectroscopy, transmission electron microscopy and selected area electron diffraction examinations showed that the CaP formed was amorphous. Inductively coupled plasma optical emission spectroscopy analysis revealed similar amounts of Ca and P in both composite types, while a smaller amount of Mg (Ca:Mg molar ratio = 10) was detected in the composites formed in the CaGP:MgGP solution, which was supported by the results of energy-dispersive x-ray spectroscopy-based elemental mapping. Scanning electron microscopy (SEM) imaging showed that the mineral deposits in PRF incubated in the CaGP:MgGP solution were markedly smaller. The mass percentage attributable to the mineral phase was similar in both composite types. MTT and WST tests with SAOS-2 cells revealed that incubation in the CaGP:MgGP solution had no negative effect on cytocompatibility and cell proliferation compared to the CaGP solution. Cells on all samples displayed a well-spread morphology as revealed by SEM imaging. In conclusion, the incorporation of Mg reduces mineral deposit dimensions and promotes cell proliferation.

  8. Management of pulpal floor perforation and grade II Furcation involvement using mineral trioxide aggregate and platelet rich fibrin: A clinical report

    Directory of Open Access Journals (Sweden)

    Rhythm Bains

    2012-01-01

    Full Text Available To report the management of an iatrogenic perforation of pulpal floor in the furcation of mandibular first molar, using Mineral Trioxide Aggregate (MTA and platelet rich fibrin (PRF. Unpredictable endodontic root/pulp chamber floor perforations resulting in unacceptable high rate of clinical failure has now been a lesser threat with the advent of new technologies and biocompatible materials that utilize the applications of basic research along with tissue engineering concept in clinical practice. Present case report illustrates the use of MTA and platelet rich fibrin (PRF for the repair of the perforation defect and regeneration of the lost periodontium in furcation area. Although, histologic events and reaction of MTA with PRF is not studied so far, however, the autologous and biocompatible nature of the components used for present treatment modalities seems to be beneficial for the long term clinical results obtained in our case.

  9. Laterally positioned flap-revised technique along with platelet rich fibrin in the management of Miller class II gingival recession

    Directory of Open Access Journals (Sweden)

    Jagmohan Singh

    2013-01-01

    Full Text Available Gingival recession is a complex phenomenon that may present numerous therapeutic challenges to the clinician. The laterally positioned flap is commonly used to cover isolated, denuded roots that have adequate donor tissue laterally and vestibular depth. Various modifications in laterally sliding flap have been proposed in order to avoid the reported undesirable results on the donor teeth. Recently, use of growth factors has been proposed in combination with surgical techniques. This article highlights the use of laterally positioned pedicle flap-revised technique as a modification of laterally sliding flap technique along with autologous suspension of growth factors, platelet rich fibrin membrane (PRF for the management of localized Miller class-II gingival recession. After 6 months of follow-up, the clinical condition was stable with 80% root coverage and satisfactory gingival tissue healing at both donor and recipient site with no signs of inflammation. An excellent esthetical outcome was achieved and the patient was satisfied with case resolution.

  10. Influence of platelet-derived growth factor-AB on tissue development in autologous platelet-rich plasma gels.

    Science.gov (United States)

    Wirz, Simone; Dietrich, Maren; Flanagan, Thomas C; Bokermann, Gudrun; Wagner, Wolfgang; Schmitz-Rode, Thomas; Jockenhoevel, Stefan

    2011-07-01

    Fibrin-based scaffolds are widely used in tissue engineering. We postulated that the use of platelet-rich plasma (PRP) in contrast to platelet-poor plasma and pure fibrinogen as the basic material leads to an increased release of autologous platelet-derived growth factor (PDGF)-AB, which may have a consequent positive effect on tissue development. Therefore, we evaluated the release of PDGF-AB during the production process and the course of PDGF release during cultivation of plasma gels with and w/o platelets. The influence of PDGF-AB on the proliferation rate of human umbilical cord artery smooth muscle cells (HUASMCs) was studied using XTT assay. The synthesis of extracellular matrix by HUASMCs in plasma- and fibrin gels was measured using hydroxyproline assay. The use of PRP led to an increase in autologous PDGF-AB release. Further, the platelet-containing plasma gels showed a prolonged release of growth factor during cultivation. Both PRP and platelet-poor plasma gels had a positive effect on the production of collagen. However, PDGF-AB as a supplement in medium and in pure fibrin gel had neither an effect on cell proliferation nor on the collagen synthesis rate. This observation may be due to an absence of PDGF receptors in HUASMCs as determined by flow cytometry. In conclusion, although the prolonged autologous production of PDGF-AB in PRP gels is possible, the enhanced tissue development by HUASMCs within such gels is not PDGF related.

  11. Effect of intraoperative platelet-rich plasma and fibrin glue application on skin flap survival.

    Science.gov (United States)

    Findikcioglu, Fulya; Findikcioglu, Kemal; Yavuzer, Reha; Lortlar, Nese; Atabay, Kenan

    2012-09-01

    The experiment was designed to compare the effect of intraoperative platelet-rich plasma (PRP) and fibrin glue application on skin flap survival. In this study, bilateral epigastric flaps were elevated in 24 rats. The right-side flaps were used as the control of the left-side flaps. Platelet-rich plasma, fibrin glue, and thrombin had been applied under the flap sites in groups 1, 2, and 3, respectively. Five days later, all flap pedicles were ligated. Necrotic area measurements, microangiography, and histologic and immunohistochemical evaluations were performed to compare the groups. Platelet-rich plasma reduced necrotic area percentages as compared with other groups. Histologically and microangiographically increased number of arterioles were observed in PRP groups. Thrombin when used alone increased flap necrosis. Vascular endothelial growth factor, platelet-derived growth factor, and transforming growth factor β3 primary antibody staining showed increased neovascularization and reepithelialization in all PRP-applied flaps. This study demonstrated that PRP, when applied intraoperatively under the skin flap, may enhance flap survival. Thrombin used alone was found to be unsuitable in flap surgery.

  12. Use of photoactivated disinfection and platelet-rich fibrin in regenerative Endodontics

    Directory of Open Access Journals (Sweden)

    Dexton Antony Johns

    2014-01-01

    Full Text Available Aim: Photoactivated disinfection has been used as an adjunct to conventional endodontic treatment. Its use in regenerative endodontics is not reported in literature. The aim of this case report was to describe a new proposal for pulp revascularization with disinfection of pulp canal space using a unique combination of a photosensitizer solution and low-power laser light. Materials and Methods: A 9-year-old boy came with the chief complaint of discolored upper central incisors (#8, #9. A diagnosis of pulp necrosis was made on the basis of clinical and radiographic findings. The canal was irrigated with 5.25% sodium hypochlorite solution and dried with paper points. Photodynamic therapy was used to disinfect the root canal and platelet-rich fibrin was used to revitalize the pulp. Three millimeters of gray mineral trioxide aggregate was placed directly over the platelet-rich plasma clot. Three days later, the tooth was double-sealed with permanent filling materials. Results: Clinical examination revealed no sensitivity to percussion or palpation tests. Radiograph revealed continued thickening of the dentinal walls, root lengthening, regression of the peri-apical lesion and apical closure. Both the roots showed complete apical closure at the 10-month follow-up. However, the teeth were not responsive to electric pulp test. Conclusion: This report of pulp revascularization shows that disinfection with photodynamic therapy combined with platelet-rich fibrin leads to satisfactory root development in necrotic immature teeth.

  13. Lyophilized platelet-rich fibrin (PRF) promotes craniofacial bone regeneration through Runx2.

    Science.gov (United States)

    Li, Qi; Reed, David A; Min, Liu; Gopinathan, Gokul; Li, Steve; Dangaria, Smit J; Li, Leo; Geng, Yajun; Galang, Maria-Therese; Gajendrareddy, Praveen; Zhou, Yanmin; Luan, Xianghong; Diekwisch, Thomas G H

    2014-05-14

    Freeze-drying is an effective means to control scaffold pore size and preserve its composition. The purpose of the present study was to determine the applicability of lyophilized Platelet-rich fibrin (LPRF) as a scaffold for craniofacial tissue regeneration and to compare its biological effects with commonly used fresh Platelet-rich fibrin (PRF). LPRF caused a 4.8-fold±0.4-fold elevation in Runt-related transcription factor 2 (Runx2) expression in alveolar bone cells, compared to a 3.6-fold±0.2-fold increase when using fresh PRF, and a more than 10-fold rise of alkaline phosphatase levels and mineralization markers. LPRF-induced Runx2 expression only occurred in alveolar bone and not in periodontal or dental follicle cells. LPRF also caused a 1.6-fold increase in osteoblast proliferation (pPRF. When applied in a rat craniofacial defect model for six weeks, LPRF resulted in 97% bony coverage of the defect, compared to 84% for fresh PRF, 64% for fibrin, and 16% without scaffold. Moreover, LPRF thickened the trabecular diameter by 25% when compared to fresh PRF and fibrin, and only LPRF and fresh PRF resulted in the formation of interconnected trabeculae across the defect. Together, these studies support the application of lyophilized PRF as a biomimetic scaffold for craniofacial bone regeneration and mineralized tissue engineering.

  14. Platelet-rich fibrin matrix improves wound angiogenesis via inducing endothelial cell proliferation.

    Science.gov (United States)

    Roy, Sashwati; Driggs, Jason; Elgharably, Haytham; Biswas, Sabyasachi; Findley, Muna; Khanna, Savita; Gnyawali, Urmila; Bergdall, Valerie K; Sen, Chandan K

    2011-11-01

    The economic, social, and public health burden of chronic ulcers and other compromised wounds is enormous and rapidly increasing with the aging population. The growth factors derived from platelets play an important role in tissue remodeling including neovascularization. Platelet-rich plasma (PRP) has been utilized and studied for the last four decades. Platelet gel and fibrin sealant, derived from PRP mixed with thrombin and calcium chloride, have been exogenously applied to tissues to promote wound healing, bone growth, hemostasis, and tissue sealing. In this study, we first characterized recovery and viability of as well as growth factor release from platelets in a novel preparation of platelet gel and fibrin matrix, namely platelet-rich fibrin matrix (PRFM). Next, the effect of PRFM application in a delayed model of ischemic wound angiogenesis was investigated. The study, for the first time, shows the kinetics of the viability of platelet-embedded fibrin matrix. A slow and steady release of growth factors from PRFM was observed. The vascular endothelial growth factor released from PRFM was primarily responsible for endothelial mitogenic response via extracellular signal-regulated protein kinase activation pathway. Finally, this preparation of PRFM effectively induced endothelial cell proliferation and improved wound angiogenesis in chronic wounds, providing evidence of probable mechanisms of action of PRFM in healing of chronic ulcers.

  15. Lyophilized Platelet-Rich Fibrin (PRF Promotes Craniofacial Bone Regeneration through Runx2

    Directory of Open Access Journals (Sweden)

    Qi Li

    2014-05-01

    Full Text Available Freeze-drying is an effective means to control scaffold pore size and preserve its composition. The purpose of the present study was to determine the applicability of lyophilized Platelet-rich fibrin (LPRF as a scaffold for craniofacial tissue regeneration and to compare its biological effects with commonly used fresh Platelet-rich fibrin (PRF. LPRF caused a 4.8-fold ± 0.4-fold elevation in Runt-related transcription factor 2 (Runx2 expression in alveolar bone cells, compared to a 3.6-fold ± 0.2-fold increase when using fresh PRF, and a more than 10-fold rise of alkaline phosphatase levels and mineralization markers. LPRF-induced Runx2 expression only occurred in alveolar bone and not in periodontal or dental follicle cells. LPRF also caused a 1.6-fold increase in osteoblast proliferation (p < 0.001 when compared to fresh PRF. When applied in a rat craniofacial defect model for six weeks, LPRF resulted in 97% bony coverage of the defect, compared to 84% for fresh PRF, 64% for fibrin, and 16% without scaffold. Moreover, LPRF thickened the trabecular diameter by 25% when compared to fresh PRF and fibrin, and only LPRF and fresh PRF resulted in the formation of interconnected trabeculae across the defect. Together, these studies support the application of lyophilized PRF as a biomimetic scaffold for craniofacial bone regeneration and mineralized tissue engineering.

  16. Autologous Platelet Gel: An In Vitro Analysis of Platelet-Rich Plasma Using Multiple Cycles

    OpenAIRE

    Christensen, Kevin; Vang, See; Brady, Chad; Isler, Jack; Allen, Keith; Anderson, John; Holt, David

    2006-01-01

    Autologous platelet gel (APG) has become an expanding field for perfusionists. By mixing platelet-rich plasma (PRP) with thrombin and calcium, platelet gel is prepared and used in many surgical settings. There are many devices used to produce PRP. This study evaluates the Medtronic Magellan Autologous Platelet Separator. The purpose of this study was to show that processing two cycles of the same syringe could reduce the amount of blood required to produce a specific volume of PRP. Three 60-m...

  17. A STUDY ON EFFICACY OF AUTOLOGOUS PLATELET RICH PLASMA IN MYRINGOPLASTY

    Directory of Open Access Journals (Sweden)

    Sankaranarayanan Gopalakrishnan

    2013-06-01

    Full Text Available Objective: To study the efficacy and advantage in using autologus platelet rich plasma during myringoplasty in closure of tympanic membrane perforation preventing the graft displacement, promoting quicker healing & improving overall outcome.Methods : Platelet rich plasma(PRP is an autologus platelet rich concentrate prepared from patients own blood with growth factors up to 8 times that of normal serum and its efficacy when used during myringoplasty is studied.50 patients with chronic otitis media inactive mucosal disease were randomly chosen and 25 of them was the study group and other 25 were the control group. Both group patients underwent myringoplasty and PRP was used in the study group and the results were evaluated.Results: In our study among 25 cases that underwent myringoplasty with use of platelet rich fibrin, 24 had complete tympanic membrane closure and only one failure has been noticed. In controls 5 out of 25 cases had failure. The graft take up rate in our study is comparable with the reference studies. Use of PRP accelerates graft uptake.Conclusion: Platelet rich plasma is a cheap and cost effective platelet concentrate with enriched growth factors. It accelerates the tympanic membrane closure following myringoplasty.

  18. Concentrated red bone marrow/platelet-rich fibrin compound with an autologous periosteum debris scaffold for repair of mandibular defects%浓缩红骨髓/富血小板纤维蛋白复合载自体骨膜碎片修复下颌骨缺损★

    Institute of Scientific and Technical Information of China (English)

    郭灵雨; 刘凯; 刘树发

    2013-01-01

    BACKGROUND: The platelet-rich fibrin scaffold structure is conducive to the growth of red bone marrow mesenchymal stem cel s and various growth factors, promoting final osteogenesis. OBJECTIVE: To investigate the feasibility of the concentrated red bone marrow/platelet-rich fibrin compound with the autologous periosteum debris scaffold for repair of rabbit mandibular defects. METHODS: Bilateral mandibular bone defect models were prepared in New Zealand white rabbits using self-control method. The left side was the experimental side, and implanted with the concentrated red bone marrow/platelet-rich fibrin compound with the autologous periosteum debris scaffolds; the right side was the control side, and implanted with periosteum fragments and nano-hydroxyapatite composite scaffolds. Al experimental animals were sacrificed after 2, 4, 8, 12 weeks to prepare tissue samples for general observation, imaging analysis, hematoxylin-eosin staining and scanning electron microscope detection. RESULTS AND CONCLUSION: The imaging examination and histological staining showed that the experimental side was superior to the control side in the aspects of bone healing, osteogenesis speed and quality. Under the scanning electron microscope, the experimental side had better compatibility with surrounding tissues and no inflammation reaction. The data of the tooth CT and evidence of new bone formation showed the bone density and new bone area of the experimental side were significantly higher than that of the control side (P < 0.05). These data indicate that concentrated red bone marrow/platelet-rich fibrin compound with the autologous periosteum debris scaffolds has an obvious osteoinductive role, which is expected to be a new-type material for clinical repair of mandibular defects.%  背景:富血小板纤维蛋白支架结构有利于红骨髓中间充质干细胞及各种生长因子的生长,促进最终成骨。目的:探讨浓缩红骨髓/富血小板纤维蛋白复合

  19. Effect of autologous platelet-rich plasma application on cutaneous wound healing in dogs

    OpenAIRE

    Jee, Cho-Hee; Eom, Na-Young; Jang, Hyo-Mi; Jung, Hae-Won; Choi, Eul-Soo; Won, Jin-Hee; Hong, Il-Hwa; Kang, Byeong-Teck; Jeong, Dong Wook; Jung, Dong-In

    2016-01-01

    This study was conducted to identify the effectiveness of platelet-rich plasma (PRP) and efficacy of intralesional injection as a method of application to acute cutaneous wounds in dogs. Healthy adult beagles (n = 3) were used in this study. Autologous PRP was separated from anticoagulant treated whole blood in three dogs. Cutaneous wounds were created and then treated by intralesional injection of PRP in the experimental group, while they were treated with saline in the control group on days...

  20. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features.

    Science.gov (United States)

    Dohan, David M; Choukroun, Joseph; Diss, Antoine; Dohan, Steve L; Dohan, Anthony J J; Mouhyi, Jaafar; Gogly, Bruno

    2006-03-01

    Platelet-rich fibrin (PRF) belongs to a new generation of platelet concentrates, with simplified processing and without biochemical blood handling. In this second article, we investigate the platelet-associated features of this biomaterial. During PRF processing by centrifugation, platelets are activated and their massive degranulation implies a very significant cytokine release. Concentrated platelet-rich plasma platelet cytokines have already been quantified in many technologic configurations. To carry out a comparative study, we therefore undertook to quantify PDGF-BB, TGFbeta-1, and IGF-I within PPP (platelet-poor plasma) supernatant and PRF clot exudate serum. These initial analyses revealed that slow fibrin polymerization during PRF processing leads to the intrinsic incorporation of platelet cytokines and glycanic chains in the fibrin meshes. This result would imply that PRF, unlike the other platelet concentrates, would be able to progressively release cytokines during fibrin matrix remodeling; such a mechanism might explain the clinically observed healing properties of PRF.

  1. Treatment of a Refractory Skin Ulcer Using Punch Graft and Autologous Platelet-Rich Plasma

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    Mauro Carducci

    2016-01-01

    Full Text Available Background. Chronic ulceration of the lower legs is a relatively common condition amongst adults: one that causes pain and social distress and results in considerable healthcare and personal costs. The technique of punch grafting offers an alternative approach to the treatment of ulcers of the lower limbs. Objective. Combining platelet-rich plasma and skin graft enhances the efficacy of treating chronic diabetic wounds by enhancing healing rate and decreasing recurrence rate. Platelet-rich plasma could, by stimulating dermal regeneration, increase the take rate after skin grafting or speed up reepithelialization. Methods and Materials. The ulcer was prepared by removing fibrin with a curette and the edges of the ulcer were freshened. The platelet-rich plasma has been infiltrated on the bottom and edges of the ulcer. The punch grafts were placed in 5 mm holes arranged. The ulcer was medicated with hydrogel and a pressure dressing was removed after 8 days. Results. After a few days the patient did not report more pain. Granulation tissue appeared quickly between implants. Most of the grafts were viable in 2-3 weeks. The grafts gradually came together to close the ulcer and were completed in four months.

  2. Posıtıve effect of platelet rich fibrin on osseointegration

    Science.gov (United States)

    Bayram, Burak; Kantarcı, Alpdoğan; Gülsever, Serap; Alaaddinoğlu, Emine-Elif

    2016-01-01

    Background Leukocyte-platelet rich fibrin (L-PRF) is a second generation platelet concentrate clinically used to accelerate tissue healing and bone regeneration. Achieving reduced implant osseointegration time could provide immediate or early loading of implants. The aim of this study was to evaluate the L-PRF-induced osseointegration and bone-implant contact (BIC) in an experimental animal model. Material and Methods Twelve 4-month-old New Zealand white rabbits were used. Following general anesthesia, 3-5 mL of blood was obtained from the central artery in rabbit ear and L-PRF was prepared. Two implant cavities (5 mm long and 3 mm in diameter) were created in each tibia with a total of four cavities in each animal. Two of these cavities were selected and covered with PRF (test group). The remaining L-PRF was used to soak the implants placed into the L-PRF covered sockets. Other cavities were left as controls. In total, 48 implants were placed. Animals were sacrificed after two, three, or four weeks. Histological samples were obtained and peri-implant tissues were histomorphometrically evaluated for bone-to-implant contact and new bone formation. Results Histomorphometric analyses of the defects revealed that the L-PRF was detectable up to the second week. Application of L-PRF increased the rate and amount of new bone formation in the experimental group compared to the control group. Bone-to-implant contact was enhanced when the surface was pre-wetted with L-PRF (pPRF application may increases amount and rate of new bone formation during the early healing period and provides a faster osseointegration around implants. Key words:Dental implants, platelet rich fibrin, osseointegration, bone regeneration, matrix for growth factors. PMID:27475686

  3. Platelet-rich fibrin-mediated revitalization of immature necrotic tooth

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    Navin Mishra

    2013-01-01

    Full Text Available Contemporary studies have shown that the regeneration of tissues and root elongation is possible in necrotic immature permanent teeth. The purpose of this case report is to add a new vista in regenerative endodontic therapy by using platelet rich fibrin for revitalization of immature non vital tooth. An 11year old boy with the history of trauma was diagnosed with the pulpal necrosis and symptomatic apical periodontitis in tooth #21. Intra oral periapical radiograph showed open apex and associated immature supernumerary tooth with respect to tooth #21. Access preparation and minimal instrumentation was done to remove necrotic debris under copious irrigation with 2.5% sodium hypochlorite. Triple antibiotic paste was packed in the canal for four weeks. During second visit, 5 mL of whole blood was drawn from the medial cubital vein of the patient and blood was then subjected to centrifugation at 2400 rpm for 12 minutes for the preparation of Platelet rich fibrin (PRF utilizing Choukroun′s method. Triple antibiotic paste was removed and canal was dried. PRF clot was pushed to the apical region of tooth #21 using hand pluggers. Three milimetres of Mineral trioxide (MTA was placed in cervical part of the root canal and permanent restoration was done three days later. Clinical examination at 6 and 12 months revealed no sensitivity to percussion and palpation in tooth #21and it responded positively to both electric pulp and cold tests. Radiographic examination showed resolution of periapical rarefaction, further root development and apical closure of the tooth #21 and its associated supernumerary tooth. On the basis of successful outcome of the present case it can be stated that PRF clot may serve as a scaffold for regeneration of necrotic immature teeth.

  4. Posıtıve effect of platelet rich fibrin on osseointegration

    Science.gov (United States)

    Bayram, Burak; Kantarcı, Alpdoğan; Gülsever, Serap; Alaaddinoğlu, Emine-Elif

    2016-01-01

    Background Leukocyte-platelet rich fibrin (L-PRF) is a second generation platelet concentrate clinically used to accelerate tissue healing and bone regeneration. Achieving reduced implant osseointegration time could provide immediate or early loading of implants. The aim of this study was to evaluate the L-PRF-induced osseointegration and bone-implant contact (BIC) in an experimental animal model. Material and Methods Twelve 4-month-old New Zealand white rabbits were used. Following general anesthesia, 3-5 mL of blood was obtained from the central artery in rabbit ear and L-PRF was prepared. Two implant cavities (5 mm long and 3 mm in diameter) were created in each tibia with a total of four cavities in each animal. Two of these cavities were selected and covered with PRF (test group). The remaining L-PRF was used to soak the implants placed into the L-PRF covered sockets. Other cavities were left as controls. In total, 48 implants were placed. Animals were sacrificed after two, three, or four weeks. Histological samples were obtained and peri-implant tissues were histomorphometrically evaluated for bone-to-implant contact and new bone formation. Results Histomorphometric analyses of the defects revealed that the L-PRF was detectable up to the second week. Application of L-PRF increased the rate and amount of new bone formation in the experimental group compared to the control group. Bone-to-implant contact was enhanced when the surface was pre-wetted with L-PRF (p<0.01). Conclusions The results of this study demonstrated that L-PRF application may increases amount and rate of new bone formation during the early healing period and provides a faster osseointegration around implants. Key words:Dental implants, platelet rich fibrin, osseointegration, bone regeneration, matrix for growth factors. PMID:27475686

  5. In search of a consensus terminology in the field of platelet concentrates for surgical use: platelet-rich plasma (PRP), platelet-rich fibrin (PRF), fibrin gel polymerization and leukocytes.

    Science.gov (United States)

    Dohan Ehrenfest, David M; Bielecki, Tomasz; Mishra, Allan; Borzini, Piero; Inchingolo, Francesco; Sammartino, Gilberto; Rasmusson, Lars; Evert, Peter A

    2012-06-01

    In the field of platelet concentrates for surgical use, most products are termed Platelet-Rich Plasma (PRP). Unfortunately, this term is very general and incomplete, leading to many confusions in the scientific database. In this article, a panel of experts discusses this issue and proposes an accurate and simple terminology system for platelet concentrates for surgical use. Four main categories of products can be easily defined, depending on their leukocyte content and fibrin architecture: Pure Platelet-Rich Plasma (P-PRP), such as cell separator PRP, Vivostat PRF or Anitua's PRGF; Leukocyteand Platelet-Rich Plasma (L-PRP), such as Curasan, Regen, Plateltex, SmartPReP, PCCS, Magellan, Angel or GPS PRP; Pure Plaletet-Rich Fibrin (P-PRF), such as Fibrinet; and Leukocyte- and Platelet-Rich Fibrin (L-PRF), such as Choukroun's PRF. P-PRP and L-PRP refer to the unactivated liquid form of these products, their activated versions being respectively named P-PRP gels and L-PRP gels. The purpose of this search for a terminology consensus is to plead for a more serious characterization of these products. Researchers have to be aware of the complex nature of these living biomaterials, in order to avoid misunderstandings and erroneous conclusions. Understanding the biomaterials or believing in the magic of growth factors ? From this choice depends the future of the field.

  6. Surgical Re-entry of an Intentionally Replanted Periodontally Compromised Tooth Treated with Platelet Rich Fibrin (PRF): Hopeless to Hopeful.

    Science.gov (United States)

    Ryana, Haneet Kour; Srinath, Rashmi; Prakash, Shobha

    2016-06-01

    Intentional replantation is generally contraindicated in periodontally compromised teeth however, there are reports suggesting that it can be a successful treatment alternative for periodontally involved hopeless teeth. Currently there is dearth of evidence regarding the success of this therapy, especially evidence for the effectiveness of autologous platelet rich fibrin is lacking. We present a case report of a 23-year-old male patient with periodontally hopeless left maxillary central incisor having bone loss extending beyond root apex. The tooth was gently extracted and replanted utilizing root conditioning and combined regenerative therapy (Xenograft, PRF and Type I Collagen Membrane). Surgical re-entry at nine months revealed bone formation in the apical third of the tooth. At one year, 87% radiographic bone gain was accomplished. The improvement in the clinical and radiographic parameters reinforced by the re-entry surgery findings strongly suggest that intentional replantation may be a cost-effective substitute to implants and tooth supported prosthesis in situations where conventional periodontal therapy would yield compromised outcomes. PMID:27504421

  7. Surgical Re-entry of an Intentionally Replanted Periodontally Compromised Tooth Treated with Platelet Rich Fibrin (PRF): Hopeless to Hopeful.

    Science.gov (United States)

    Ryana, Haneet Kour; Srinath, Rashmi; Prakash, Shobha

    2016-06-01

    Intentional replantation is generally contraindicated in periodontally compromised teeth however, there are reports suggesting that it can be a successful treatment alternative for periodontally involved hopeless teeth. Currently there is dearth of evidence regarding the success of this therapy, especially evidence for the effectiveness of autologous platelet rich fibrin is lacking. We present a case report of a 23-year-old male patient with periodontally hopeless left maxillary central incisor having bone loss extending beyond root apex. The tooth was gently extracted and replanted utilizing root conditioning and combined regenerative therapy (Xenograft, PRF and Type I Collagen Membrane). Surgical re-entry at nine months revealed bone formation in the apical third of the tooth. At one year, 87% radiographic bone gain was accomplished. The improvement in the clinical and radiographic parameters reinforced by the re-entry surgery findings strongly suggest that intentional replantation may be a cost-effective substitute to implants and tooth supported prosthesis in situations where conventional periodontal therapy would yield compromised outcomes.

  8. Surgical Re-entry of an Intentionally Replanted Periodontally Compromised Tooth Treated with Platelet Rich Fibrin (PRF): Hopeless to Hopeful

    Science.gov (United States)

    Srinath, Rashmi; Prakash, Shobha

    2016-01-01

    Intentional replantation is generally contraindicated in periodontally compromised teeth however, there are reports suggesting that it can be a successful treatment alternative for periodontally involved hopeless teeth. Currently there is dearth of evidence regarding the success of this therapy, especially evidence for the effectiveness of autologous platelet rich fibrin is lacking. We present a case report of a 23-year-old male patient with periodontally hopeless left maxillary central incisor having bone loss extending beyond root apex. The tooth was gently extracted and replanted utilizing root conditioning and combined regenerative therapy (Xenograft, PRF and Type I Collagen Membrane). Surgical re-entry at nine months revealed bone formation in the apical third of the tooth. At one year, 87% radiographic bone gain was accomplished. The improvement in the clinical and radiographic parameters reinforced by the re-entry surgery findings strongly suggest that intentional replantation may be a cost-effective substitute to implants and tooth supported prosthesis in situations where conventional periodontal therapy would yield compromised outcomes. PMID:27504421

  9. Microbicidal properties of Leukocyte- and Platelet-Rich Plasma/Fibrin (L-PRP/L-PRF): new perspectives.

    Science.gov (United States)

    Cieslik-Bielecka, A; Dohan Ehrenfest, D M; Lubkowska, A; Bielecki, T

    2012-01-01

    Platelets, as main actors of the first stage of the healing process, play an important role in tissue repair. Their granules contain many active substances, particularly over 30 growth factors with significant effects on the resident cells at the site of injury, such as mesenchymal stem cells, chondrocytes, fibroblasts, osteoblasts. This potential may be increased by the concentration of the platelets, using platelet-rich plasma/fibrin products. In the four families of platelet concentrates, 2 families contain also significant concentrations of leukocytes: L-PRP (Leukocyte- and Platelet-Rich Plasma) and L-PRF (Leukocyte- and Platelet-Rich Fibrin). Inductive properties of platelet concentrates were widely described. However, they present also antimicrobial effects. The antibacterial effects of L-PRP were highlighted in only a few in vitro studies. Strong activity comparable to gentamicin and oxacillin for L-PRP against methicillin susceptible Staphylococcus aureus (MSSA) was already demonstrated. L-PRP also inhibited the growth of methicillin resistant Staphylococcus aureus (MRSA) and Escherichia coli. Some authors also reported clinical observations about the reduction of infections and the induction of healing processes after the use of platelet concentrates in cardiac, orthopaedic, oral and maxillofacial surgery. However, very little is yet known about the antibacterial effects of these concentrates. In this manuscript, the current data about the antimicrobial agents and cells present in the platelet-rich plasma/fibrin are highlighted and discussed, in order to introduce this new key chapter of the platelet concentrate technology history.

  10. Effects of platelet-poor plasma, platelet-rich plasma, and platelet-rich fibrin on healing of extraction sockets with buccal dehiscence in dogs.

    Science.gov (United States)

    Hatakeyama, Ichiro; Marukawa, Eriko; Takahashi, Yukinobu; Omura, Ken

    2014-02-01

    Alveolar bone resorption generally occurs during healing after tooth extraction. This study aimed to evaluate the effects of platelet-poor plasma (PPP), platelet-rich plasma (PRP), and platelet-rich fibrin (PRF) on healing in a ridge-augmentation model of the canine socket with dehiscence of the buccal wall. The third mandibular premolars of 12 beagle dogs were extracted and a 3 mm buccal dehiscence from the alveolar crest to the buccal wall of the extraction socket was created. These sockets were then divided into four groups on the basis of the material used to fill the sockets: PPP, PRP, PRF, and control (no graft material) groups. Results were evaluated at 4 and 8 weeks after surgery. The ultrastructural morphology and constructs of each blood product were studied by a scanning electron microscope (SEM) or calculating concentrations of platelets, fibrinogen, platelet-derived growth factor, and transforming growth factor-β. A total of five microcomputed tomography images of specimens were selected for measurement, and the area occupied by the newly formed bone as well as the horizontal bone width were measured. Moreover, decalcified tissue specimens from each defect were analyzed histologically. The median area of new bone at 4 and 8 weeks and median horizontal bone width at 8 weeks were the highest in the PPP group. However, bone maturation in the PRF and the PRP groups was more progressed than that in the PPP and control groups. By SEM findings, the PRF group showed a more highly condensed fibrin fiber network that was regularly arranged when compared with the PPP and PRP groups. The growth factors released from platelets in PRP indicated higher concentrations than that in PRF. Under more severe conditions for bone formation, as in this experiment, the growth factors released from platelets had a negative effect on bone formation. This study showed that PPP is an effective material for the preservation of sockets with buccal dehiscence.

  11. Bone neoformation in defects treated with fibrin platelet rich membrane versus collagen membrane: a histomorphometric study in rabbit’s femurs.

    Directory of Open Access Journals (Sweden)

    Edwin Jonathan Meza

    2015-02-01

    Full Text Available The aim of the present research was to compare the bone neoformation in bone defects treated with platelet rich fibrin (PRF and collagen membrane (CM, after 3 and 5 weeks for which two bone defects were created of 4 mm width and 6 mm depth in the left femur distal diaphysis of New Zeland rabbits (n = 12. The subjects were randomly allocated in 2 groups. One of the defects was covered with a platelet rich fibrin membrane (Centrifuged resorbable Autologous blood biopolymer without biochemical modification or collagen membrane (gold standard – Neo Mem. The second defect was left uncovered (NC. The rabbits were sacrified after 3 and 5 weeks (3 rabbits per period. The femur was completely removed and they were processed histomophometrically. The bone neoformation analysis was performed through a differential points counting. The data was statistically analyzed (ANOVA, Tukey. The histomorphometric results showed that bone neoformation of the defects treated with PRF after 3 weeks was equivalent to the neoformation of the CM (p

  12. Classification of platelet concentrates (Platelet-Rich Plasma-PRP, Platelet-Rich Fibrin-PRF) for topical and infiltrative use in orthopedic and sports medicine: current consensus, clinical implications and perspectives.

    Science.gov (United States)

    Dohan Ehrenfest, David M; Andia, Isabel; Zumstein, Matthias A; Zhang, Chang-Qing; Pinto, Nelson R; Bielecki, Tomasz

    2014-01-01

    Platelet concentrates for topical and infiltrative use - commonly termed Platetet-Rich Plasma (PRP) or Platelet-Rich Fibrin (PRF) - are used or tested as surgical adjuvants or regenerative medicine preparations in most medical fields, particularly in sports medicine and orthopaedic surgery. Even if these products offer interesting therapeutic perspectives, their clinical relevance is largely debated, as the literature on the topic is often confused and contradictory. The long history of these products was always associated with confusions, mostly related to the lack of consensual terminology, characterization and classification of the many products that were tested in the last 40 years. The current consensus is based on a simple classification system dividing the many products in 4 main families, based on their fibrin architecture and cell content: Pure Platelet-Rich Plasma (P-PRP), such as the PRGF-Endoret technique; Leukocyte- and Platelet-Rich Plasma (LPRP), such as Biomet GPS system; Pure Platelet-Rich Fibrin (P-PRF), such as Fibrinet; Leukocyte- and Platelet-Rich Fibrin (L-PRF), such as Intra-Spin L-PRF. The 4 main families of products present different biological signatures and mechanisms, and obvious differences for clinical applications. This classification serves as a basis for further investigations of the effects of these products. Perspectives of evolutions of this classification and terminology are also discussed, particularly concerning the impact of the cell content, preservation and activation on these products in sports medicine and orthopaedics.

  13. Classification of platelet concentrates (Platelet-Rich Plasma-PRP, Platelet-Rich Fibrin-PRF) for topical and infiltrative use in orthopedic and sports medicine: current consensus, clinical implications and perspectives

    Science.gov (United States)

    Dohan Ehrenfest, David M.; Andia, Isabel; Zumstein, Matthias A.; Zhang, Chang-Qing; Pinto, Nelson R.; Bielecki, Tomasz

    2014-01-01

    Summary Platelet concentrates for topical and infiltrative use – commonly termed Platetet-Rich Plasma (PRP) or Platelet-Rich Fibrin (PRF) – are used or tested as surgical adjuvants or regenerative medicine preparations in most medical fields, particularly in sports medicine and orthopaedic surgery. Even if these products offer interesting therapeutic perspectives, their clinical relevance is largely debated, as the literature on the topic is often confused and contradictory. The long history of these products was always associated with confusions, mostly related to the lack of consensual terminology, characterization and classification of the many products that were tested in the last 40 years. The current consensus is based on a simple classification system dividing the many products in 4 main families, based on their fibrin architecture and cell content: Pure Platelet-Rich Plasma (P-PRP), such as the PRGF-Endoret technique; Leukocyte- and Platelet-Rich Plasma (LPRP), such as Biomet GPS system; Pure Platelet-Rich Fibrin (P-PRF), such as Fibrinet; Leukocyte- and Platelet-Rich Fibrin (L-PRF), such as Intra-Spin L-PRF. The 4 main families of products present different biological signatures and mechanisms, and obvious differences for clinical applications. This classification serves as a basis for further investigations of the effects of these products. Perspectives of evolutions of this classification and terminology are also discussed, particularly concerning the impact of the cell content, preservation and activation on these products in sports medicine and orthopaedics. PMID:24932440

  14. Sinus Augmentation by Platelet-Rich Fibrin Alone: A Report of Two Cases with Histological Examinations

    Directory of Open Access Journals (Sweden)

    Naofumi Aoki

    2016-01-01

    Full Text Available In sinus floor augmentation of an atrophic posterior maxilla, platelet-rich fibrin (PRF has been used as a graft material. We herein report two cases with histological evaluations of PRF after the surgery. The first case was a 28-year-old female with an atrophic right posterior maxilla who was treated with sinus floor augmentation and simultaneous implant placement using PRF as the sole graft material in our hospital. Twenty-four months after surgery, the implant was unfortunately removed because of occlusal overloading by parafunctional habits. During implant replacement, a tissue sample was obtained from the site of augmentation with PRF and was evaluated histologically. The second case was a 58-year-old man with severe alveolar atrophy of the right maxilla who underwent lateral sinus augmentation using only PRF in a two-stage procedure in our hospital. Samples were obtained at the second-stage surgery and histological examinations were performed. As a result, new bone formation was confirmed histologically in both cases. Our findings show that the use of PRF as a graft material during sinus floor augmentation induces natural bone regeneration.

  15. The Effect of Platelet-rich Fibrin Matrix on Rotator Cuff Healing in a Rat Model.

    Science.gov (United States)

    Hasan, S; Weinberg, M; Khatib, O; Jazrawi, L; Strauss, E J

    2016-01-01

    The purpose of the current study was to determine if the application of platelet-rich fibrin matrix could improve regeneration of the tendon-bone insertion site in a rat rotator cuff repair model. 25 Lewis syngeneic rats underwent bilateral tenotomy and repair of the supraspinatus tendon. 10 separate rats were used for PRFM harvest. All left (control) shoulders underwent transosseous rotator cuff repair, while all right (treatment) shoulders were repaired similarly with PRFM augmentation. 9 rats were sacrificed at 2-weeks and ten at 4-weeks for biomechanical testing. 3 separate rats were sacrificed at 2-weeks and 4-weeks each for histologic analysis of the insertion site. At 2 weeks, the experimental group repairs were significantly stronger in ultimate load to failure (P=0.01), stress (P=0.03), and stiffness (P=0.03). Differences in biomechanical testing were not found between the groups at 4 weeks. Histological analysis revealed less collagen organization and cartilage formation at the insertion site in the experimental group. Semiquantitative histologic analysis confirmed our qualitative assessment of the specimens. PRFM does not recapitulate the native enthesis, but rather induces an exuberant and disordered healing response that is characterized by fibrovascular scar tissue. PMID:26509369

  16. Clinical and Histologic Evaluation of Platelet-Rich Fibrin Accelerated Epithelization of Gingival Wound

    Science.gov (United States)

    Bansal, Mansi; Kumar, Ashish; Puri, Komal; Khatri, Manish; Gupta, Geeti; Vij, Hitesh

    2016-01-01

    The foremost indication for gingival depigmentation is patient demand for improved aesthetics. In most cases after the removal of pigmented layer, the area is covered with periodontal packs. These dressings have no curative properties. They only minimise the likelihood of surface trauma during mastication. However, platelet-rich fibrin (PRF) accelerates wound healing by effective neovascularisation and promoting fast cicatricial tissue remodelling. In the present split mouth study, PRF membrane was applied in the first quadrant and non-eugenol dressing (Coe-Pack) in the second quadrant after depigmentation. Clinical evaluation of epithelization with toluidine blue revealed that PRF treated sites stained substantially less indicating better wound healing as compared to Coe-Pack sites, which appeared more erythematous after 5 days. The histologic evaluation also revealed greater inflammatory cell infiltrate on Coe-Pack sites as compared to PRF. Thus, PRF membrane as a periodontal dressing is a successful approach to protect the raw wound area of the depigmented site to reduce healing time and patient discomfort.

  17. A comparative evaluation of the blood clot, platelet-rich plasma, and platelet-rich fibrin in regeneration of necrotic immature permanent teeth: A clinical study

    Directory of Open Access Journals (Sweden)

    Isha Narang

    2015-01-01

    Full Text Available Introduction: This study was designed as a clinical trial to evaluate and compare the regenerative potential of platelet-rich fibrin (PRF, platelet-rich plasma (PRP, and blood clot in immature necrotic permanent teeth with or without associated apical periodontitis. Methods: Access preparation was done under rubber dam isolation. Copious irrigation was done with 2.5% NaOCl and triple antibiotic paste was placed as an intracanal medicament. After 4 weeks, the cases were divided into four groups with five patients in each group. The study design had three test arms and one control arm. Group I in which mineral trioxide aggregate apexification was carried out and it was kept as control group to evaluate the regenerative potential of blood clot and platelet concentrates, Group II in which blood clot was used as scaffold in the canal, Group III in PRF was used as scaffold, and Group IV in which PRP carried on collagen was used as a scaffold. Results: The clinical and radiographic evaluation after 6 and 18 months was done by two independent observers who were blinded from the groups. The scoring was done as: None score was denoted by, Fair by 1, Good by 2, and Excellent by 3. The data were then analyzed statistically by Fisher′s exact test using Statistics and Data 11.1(PRP Using harvest Smart PReP2 which showed statistically significant values in Group III as compared to other Groups. Conclusion: PRF has huge potential to accelerate the growth characteristics in immature necrotic permanent teeth as compared to PRP and blood clot.

  18. Biocompatibility, biodegradation, and neovascularization of human single-unit platelet-rich fibrin glue: an in vivo analysis

    Institute of Scientific and Technical Information of China (English)

    Wu Xiuwen; Ren Jianan; Yao Genhong; Zhou Bo; Wang Gefei; Gu Guosheng; Luan Jianfeng

    2014-01-01

    Background The clinical applications of fibrin glue span over several surgical modalities.The aim of this study was to evaluate the biocompatibility and biodegradation of different formulations of platelet-rich fibrin glue in vivo and examine its effects on the neovascularization of wound sites.Methods Human-derived single-unit fibrin glue was prepared.Incisions were made on the backs of rats,and these were coated with homemade glues containing different concentrations of aminomethylbenzoic acid (Groups A-F) or commercial adhesives (Group G).A sham control group was included (Group H).The wounds were examined by histological analysis and immunohistochemistry at several time points.Results Successful wound closure was achieved in all groups by day 12.Acute inflammation occurred during the first six days,but gradually disappeared.The longest sealant duration was achieved using the lowest concentration of antifibrinolytic agent in a 1:10 volume ratio with cryoprecipitate.Expression levels of the platelet endothelial cell adhesion molecule-1 were significantly higher in Groups A and C compared to the control groups (Groups G and H) on day 3 (P <0.05).Conclusions Single-unit platelet-rich fibrin glue has excellent biocompatibility and is associated with the upregulation of neovascularization.The addition of aminomethylbenzoic acid could prevent the degradation of fibrin glue.

  19. Prevention of Localized Osteitis in Mandibular Third-Molar Sites Using Platelet-Rich Fibrin

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    Donald R. Hoaglin

    2013-01-01

    Full Text Available Purpose. To review our experience utilizing platelet rich fibrin (PRF, which is reported to aid in wound healing of extraction sites, for the prevention of localized osteitis following lower third-molar removal. Materials and Methods. PRF was placed in the mandibular third-molar extraction sites, 200 sites total, on 100 consecutive patients treated in our practice, by the authors. The patients were managed with standard surgical techniques, intraoperative IV antibiotic/steroid coverage, and routine postoperative narcotic analgesics/short-term steroid coverage. All patients were reevaluated for localized osteitis within 7–10 days of the surgery. A comparison group consisted of 100 consecutive patients who underwent bilateral removal of indicated mandibular wisdom teeth and did not receive PRF placement within the lower third molar surgical sites. Results. The incidence of localized osteitis (LO following removal of 200 lower third molars with simultaneous PRF placement within the extraction site was 1% (2 sites out of 200. The group of patients whose mandibular 3rd molar sockets were not treated with PRF demonstrated a 9.5% (19 sites out of 200 incidence of localized osteitis. The latter group also required 6.5 hours of additional clinical time to manage LO than the study group who received PRF. Conclusions. This retrospective review demonstrated that preventative treatment of localized osteitis can be accomplished using a low cost, autogenous, soluble, biologic material, PRF, that PRF enhanced third-molar socket healing/clot retention and greatly decreased the clinical time required for postoperative management of LO.

  20. Prevention of localized osteitis in mandibular third-molar sites using platelet-rich fibrin.

    Science.gov (United States)

    Hoaglin, Donald R; Lines, Gary K

    2013-01-01

    Purpose. To review our experience utilizing platelet rich fibrin (PRF), which is reported to aid in wound healing of extraction sites, for the prevention of localized osteitis following lower third-molar removal. Materials and Methods. PRF was placed in the mandibular third-molar extraction sites, 200 sites total, on 100 consecutive patients treated in our practice, by the authors. The patients were managed with standard surgical techniques, intraoperative IV antibiotic/steroid coverage, and routine postoperative narcotic analgesics/short-term steroid coverage. All patients were reevaluated for localized osteitis within 7-10 days of the surgery. A comparison group consisted of 100 consecutive patients who underwent bilateral removal of indicated mandibular wisdom teeth and did not receive PRF placement within the lower third molar surgical sites. Results. The incidence of localized osteitis (LO) following removal of 200 lower third molars with simultaneous PRF placement within the extraction site was 1% (2 sites out of 200). The group of patients whose mandibular 3rd molar sockets were not treated with PRF demonstrated a 9.5% (19 sites out of 200) incidence of localized osteitis. The latter group also required 6.5 hours of additional clinical time to manage LO than the study group who received PRF. Conclusions. This retrospective review demonstrated that preventative treatment of localized osteitis can be accomplished using a low cost, autogenous, soluble, biologic material, PRF, that PRF enhanced third-molar socket healing/clot retention and greatly decreased the clinical time required for postoperative management of LO. PMID:23653648

  1. Platelet Rich Fibrin in the revitalization of tooth with necrotic pulp and open apex

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    Vasundara Yayathi Shivashankar

    2012-01-01

    Full Text Available Regeneration of pulp-dentin complex in an infected necrotic tooth with an open apex is possible if the canal is effectively disinfected. The purpose of this case report is to add a regenerative endodontic case to the existing literature about using Platelet Rich Fibrin (PRF. A nine year old boy who accidently broke his immature maxillary central incisor tooth, developed pulpal necrosis with apical periodontitis. After the access cavity preparation, the canal was effectively irrigated with 20 ml of 5.25% sodium hypochlorite solution and 10ml of 0.2% chlorhexidine solution and dried with paper points. Triple antibiotic paste was placed inside the canal and left for 21 days. 12 ml of whole blood was drawn from the patient′s right antecubital vein and centrifuged for 10 minutes to obtain the Choukroun′s PRF. After the removal of the triple antibiotic paste, the PRF was placed into the canal till the level of cementoenamel junction and 3mm of grey MTA was placed directly over the PRF clot. The setting of MTA was confirmed 3 days later and the tooth was double sealed with GIC and Composite restoration.After 1 year the clinical examination revealed negative responses to percussion and palpation tests. The tooth responded positively to cold and electric pulp tests. Radiographic examination revealed continued thickening of the dentinal walls, root lengthening, regression of the periapical lesion and apical closure. On the basis of the results obtained in our case report we conclude that revitalization of necrotic infected immature tooth is possible under conditions of total canal disinfection and PRF is an ideal biomaterial for pulp-dentin complex regeneration.

  2. The effect of autologous platelet rich plasma in treatment lateral epicondylitis

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    İsmail Ağır

    2011-03-01

    Full Text Available Lateral epicondylitis (tennis elbow is the most commonly diagnosed cause of lateral elbow pain. The aim of this study was to compare the effect of single dose corticosteroid and autologous platelet rich plasma (PRP injection in the treatment of tennis elbow.Materials and methods: The 15 elbow of 15 patients (6 male and 9 female was included in the study, who applied to our clinic with lateral elbow pain and diagnosed as lateral epicondylitis. A single dose of 0,5 ml Bethametasone and 0,5 ml Prilocaine mixture was applied to first group and single dose 1 ml autologous PRP was locally applied to the second group.Results: In the early follow-ups the results of corticosteroid group were better than latter follow-ups, however in PRP group the results were worst in early follow-ups but better results were obtained in later follow-ups according to Verhaar scoring system.Conclusion: According to our results, the beneficial effects of PRP injection in lateral epicondylitis increases over time but further studies with more patients and longer follow up durations should done in order to more clarified this subject.

  3. An innovative approach in the management of palatogingival groove using Biodentine™ and platelet-rich fibrin membrane.

    Science.gov (United States)

    Johns, Dexton Antony; Shivashankar, Vasundhara Yayathi; Shobha, K; Johns, Manu

    2014-01-01

    Palatogingival groove is an anatomical malformation that often causes severe periodontal defects. Treatments of such an anomaly present a clinical challenge to the operator. Careful endodontic and periodontal procedures may restore the form and function. In the present case; root canal therapy, apicectomy, and sealing of the groove with Biodentine™ were done. Bone graft was placed followed by platelet-rich fibrin (PRF) membrane. This treatment modality resulted in gain in attachment, reduction in pocket depth, and deposition of bone in the osseous defect. A 24 month follow-up is included.

  4. Platelet-rich plasma-induced bone marrow mesenchymal stem cells versus autologous nerve grafting for sciatic nerve repair

    Institute of Scientific and Technical Information of China (English)

    Changsuo Xia; Yajuan Li; Wen Cao; Zhaohua Yu

    2010-01-01

    Autologous nerve grafting is the gold standard of peripheral nerve repair.We previously showed that autologous platelet-rich plasma(PRP)contains high concentrations of growth factors and can induce in vitro cultured bone marrow mesenchymal stem cells(BMSCs)to differentiate into Schwann cells.Here we used PRP-induced BMSCs combined with chemically extracted acellular nerves to repair sciatic nerve defects and compared the effect with autologous nerve grafting.The BMSCs and chemically extracted acellular nerve promoted target muscle wet weight restoration,motor nerve conduction velocity,and axonal and myelin sheath regeneration,with similar effectiveness to autologous nerve grafting.This finding suggests that PRP induced BMSCs can be used to repair peripheral nerve defects.

  5. Current knowledge and perspectives for the use of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in oral and maxillofacial surgery part 1: Periodontal and dentoalveolar surgery.

    Science.gov (United States)

    Del Corso, Marco; Vervelle, Alain; Simonpieri, Alain; Jimbo, Ryo; Inchingolo, Francesco; Sammartino, Gilberto; Dohan Ehrenfest, David M

    2012-06-01

    Platelet concentrates for surgical use are innovative tools of regenerative medicine, and were widely tested in oral and maxillofacial surgery. Unfortunately, the literature on the topic is contradictory and the published data are difficult to sort and interpret. In periodontology and dentoalveolar surgery, the literature is particularly dense about the use of the various forms of Platelet-Rich Plasma (PRP) - Pure Platelet-Rich Plasma (P-PRP) or Leukocyte- and Platelet-Rich Plasma (L-PRP) - but still limited about Platelet-Rich Fibrin (PRF) subfamilies. In this first article, we describe and discuss the current published knowledge about the use of PRP and PRF during tooth avulsion or extraction, mucogingival surgery, Guided Tissue Regeneration (GTR) or bone filling of periodontal intrabony defects, and regeneration of alveolar ridges using Guided Bone Regeneration (GBR), in a comprehensive way and in order to avoid the traps of a confusing literature and to highlight the underlying universal mechanisms of these products. Finally, we particularly insist on the perspectives in this field, through the description and illustration of the systematic use of L-PRF (Leukocyte- and Platelet- Rich Fibrin) clots and membranes during tooth avulsion, cyst exeresis or the treatment of gingival recessions by root coverage. The use of L-PRF also allowed to define new therapeutic principles: NTR (Natural Tissue Regeneration) for the treatment of periodontal intrabony lesions and Natural Bone Regeneration (NBR) for the reconstruction of the alveolar ridges. In periodontology, this field of research will soon find his golden age by the development of user-friendly platelet concentrate procedures, and the definition of new efficient concepts and clinical protocols.

  6. Leukocyte inclusion within a platelet rich plasma-derived fibrin scaffold stimulates a more pro-inflammatory environment and alters fibrin properties.

    Science.gov (United States)

    Anitua, Eduardo; Zalduendo, Mar; Troya, María; Padilla, Sabino; Orive, Gorka

    2015-01-01

    One of the main differences among platelet-rich plasma (PRP) products is the inclusion of leukocytes that may affect the biological efficacy of these autologous preparations. The purpose of this study was to evaluate whether the addition of leukocytes modified the morphological, biomechanical and biological properties of PRP under normal and inflammatory conditions. The release of pro-inflammatory cytokines from plasma rich in growth factors (PRGF) and leukocyte-platelet rich plasma (L-PRP) scaffolds was determined by enzyme-linked immunosorbent assay (ELISA) and was significantly increased under an inflammatory condition when leukocytes were included in the PRP. Fibroblasts and osteoblasts treated with L-PRP, under an inflammatory situation, underwent a greater activation of NFĸB pathway, proliferated significantly less and secreted a higher concentration of pro-inflammatory cytokines. These cellular events were assessed through Western blot and fluorimetric and ELISA methods, respectively. Therefore, the inclusion of leukocytes induced significantly higher pro-inflammatory conditions.

  7. Platelet-rich fibrin, "a faster healing aid" in the treatment of combined lesions: A report of two cases

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    Parupalli Karunakar

    2014-01-01

    Full Text Available Anatomically the pulp and periodontium are connected through apical foramen, and the lateral, accessory, and furcal canals. Diseases of one tissue may affect the other. In the present case report with two cases, a primary periodontal lesion with secondary endodontic involvement is described. In both cases, root canal treatment was done followed by periodontal therapy with the use of platelet-rich fibrin (PRF as the regenerative material of choice. PRF has been a breakthrough in the stimulation and acceleration of tissue healing. It is used to achieve faster healing of the intrabony defects. Absence of an intraradicular lesion, pain, and swelling, along with tooth stability and adequate radiographic bone fill at 9 months of follow-up indicated a successful outcome.

  8. Guided bone regeneration (GBR) using cortical bone pins in combination with leukocyte- and platelet-rich fibrin (L-PRF).

    Science.gov (United States)

    Toffler, Michael

    2014-03-01

    Two of the fundamental requisites for guided bone regeneration (GBR) are space maintenance and primary soft-tissue closure. Allogeneic cortical bone pins measuring 2 mm in diameter in customized lengths can protect surrounding graft materials, support bioresorbable membrane barriers, and resist wound compression from the overlying soft tissues. In addition, a second-generation platelet concentrate, leukocyte- and platelet-rich fibrin (L-PRF), may be incorporated into the augmentation procedure to provide multiple growth factors, accelerate wound healing, and aid in the maintenance of primary closure over the grafted materials. Highlighting two case reports, this article features a GBR technique that uses bone pins in combination with L-PRF membranes to provide both horizontal and vertical ridge augmentation at severely compromised implant sites.

  9. Platelet-rich fibrin combined with synthetic nanocrystalline hydroxy apatite granules in the management of radicular cyst.

    Science.gov (United States)

    Pradeep, K; Kudva, Adarsh; Narayanamoorthy, Vidya; Cariappa, K M; Saraswathi, M Vidya

    2016-01-01

    Radicular cysts are inflammatory jaw cysts confined to the apices of teeth with infected and necrotic pulp. They arise from the epithelial residues in the periodontal ligament as a result of inflammation, following the death of pulp. The treatment of such lesions vary with regard to their sizes; the small cystic lesions heal after an endodontic therapy, but larger lesions, may require additional treatment. Apical surgery for radicular cysts generally involves apical root resection and sealing with endodontic material. This case report, describes the treatment of a cyst related to the maxillary central and lateral incisors using platelet rich fibrin along with synthetic nanocrystalline hydroxyapatite granules for the regeneration of lost tissues. A follow-up evaluation at 6 months and 1-year revealed a significant radiographic bone fill with satisfactory healing at the surgical site. PMID:27538563

  10. Comparison of Achilles tendon repair techniques in a sheep model using a cross-linked acellular porcine dermal patch and platelet-rich plasma fibrin matrix for augmentation.

    Science.gov (United States)

    Sarrafian, Tiffany L; Wang, Hali; Hackett, Eileen S; Yao, Jian Q; Shih, Mei-Shu; Ramsay, Heather L; Turner, A Simon

    2010-01-01

    The primary goal of this study was to evaluate a cross-linked acellular porcine dermal patch (APD), as well as platelet-rich plasma fibrin matrix (PRPFM), for repair of acute Achilles tendon rupture in a sheep model. The 2 surgically transected tendon ends were reapproximated in groups 1 and 2, whereas a gap was left between the tendon ends in group 3. APD was used to reinforce the repair in group 2, and autologous PRPFM was used to fill the gap, which was also reinforced with APD, in group 3. All sheep were humanely euthanized at 24 weeks after the repair, and biomechanical and histological testing were performed. Tensile strength testing showed a statistically significant difference in elongation between the operated limb and the unoperated contralateral limb in groups 1 and 3, but not in group 2. All operated tendons appeared healed with no apparent fibrosis under light and polarized microscopy. In group 1, all surgical separation sites were identifiable, and healing occurred via increasing tendon thickness. In group 2, healing occurred with new tendon fibers across the separation, without increasing tendon thickness in 2 out of 6 animals. Group 3 showed complete bridging of the gap, with no change in tendon thickness in 2 out of 6 animals. In groups 2 and 3, peripheral integration of the APD to tendon fibers was observed. These findings support the use of APD, alone or with PRPFM, to augment Achilles tendon repair in a sheep model.

  11. Platelet-Rich Fibrin-PRF in Dental Implant%富血小板纤维蛋白-PRF在口腔种植中的应用

    Institute of Scientific and Technical Information of China (English)

    王少华; 董卫华

    2016-01-01

    Tissue regeneration need growth factors, osteogenic cells, scaffolds and good blood supply. Wherein platelet rich fibrin (platelet-rich fibrin, PRF) platelet rich and various cytokines, with soft and hard tissue regeneration capacity of good promotion. Oral Implantology in patients with clinical situations many alveolar bone defect or lack of, PRF can promote alveolar bone defects, increased alveolar bone, soft tissue implants promote healing, inflammation around the implant treatment.%组织再生需要生长因子﹑成骨相关细胞﹑支架材料和良好的血供。其中富血小板纤维蛋白(platelet-rich fibrin,PRF)富含血小板及各种细胞因子,具有良好的促进软﹑硬组织再生的能力。临床口腔种植患者中有很多牙槽骨缺损或不足的情况﹐PRF可促进牙槽骨缺损修复﹐增高牙槽骨﹐促进种植体周围软组织愈合﹐治疗种植体周围炎。

  12. 富血小板纤维蛋白新生诱导骨的组织学观察%Histological observation on new bone induced by platelet-rich fibrin

    Institute of Scientific and Technical Information of China (English)

    付冬梅; 肖琼; 杨琴秋; 董露; 陈红亮; 孙勇

    2016-01-01

    BACKGROUND:In previous experiments, we have confirmed that platelet rich fibrin has the ability of osteoinduction, and have conducted a preliminary study on its microstructure and biomechanics. However, little is reported on its histology research. OBJECTIVE:To compare the histological changes after implanting platelet-rich fibrin, Bio-Oss and autologous bone and to analyze the pros and cons of platelet-rich fibrin implantation for repair of bone defects. METHODS: As previously reported, animal models of critical bone defects were established respectively on the bilateral femoral condyles of 12 beagle dogs. Then, platelet-rich fibrin, Bio-Oss+colagen membrane (Bio-Oss group) and autologous bone (autologous bone group)+colagen membrane were respectively implanted. At 3, 6, 8 and 12 months, one experimental dog from each group was kiled, respectively, and histological observation was performed. Another beagle dog as blank control was enroled to establish the animal model of critical bone defects, with no implantation. RESULTS AND CONCLUSION:At 3, 6, 8 and 12 months after implantation, there were significant differences in the new bone formation speed and amount between the platelet-rich fibrin group, Bio-Oss group and autologous bone group. These three kinds of bone grafts al had osteoinductive ability to different extents. In the platelet-rich fibrin group, the osteogenic effects were better at 3 and 6 months, and the new bone was similar to natural one; in the autologuos bone group, bone necrosis was noticeable at 3 and 6 months, but the osteogenic effects became better at 8 months, and the new bone was similar to natural one at 12 months; in the Bio-oss group, the osteogenic effects were similar to those in the platelet-rich fibrin group, but the residual of Bio-oss was visible at 12 months; in the blank control group, no bone formed at 3 months, indicating the animal model of critical bone defects was made successfuly. In brief, the platelet-rich fibrin has good

  13. Leukocyte inclusion within a platelet rich plasma-derived fibrin scaffold stimulates a more pro-inflammatory environment and alters fibrin properties.

    Directory of Open Access Journals (Sweden)

    Eduardo Anitua

    Full Text Available One of the main differences among platelet-rich plasma (PRP products is the inclusion of leukocytes that may affect the biological efficacy of these autologous preparations. The purpose of this study was to evaluate whether the addition of leukocytes modified the morphological, biomechanical and biological properties of PRP under normal and inflammatory conditions. The release of pro-inflammatory cytokines from plasma rich in growth factors (PRGF and leukocyte-platelet rich plasma (L-PRP scaffolds was determined by enzyme-linked immunosorbent assay (ELISA and was significantly increased under an inflammatory condition when leukocytes were included in the PRP. Fibroblasts and osteoblasts treated with L-PRP, under an inflammatory situation, underwent a greater activation of NFĸB pathway, proliferated significantly less and secreted a higher concentration of pro-inflammatory cytokines. These cellular events were assessed through Western blot and fluorimetric and ELISA methods, respectively. Therefore, the inclusion of leukocytes induced significantly higher pro-inflammatory conditions.

  14. Combination of platelet rich fibrin, hydroxyapatite and PRF membrane in the management of large inflammatory periapical lesion.

    Science.gov (United States)

    Shivashankar, Vasundara Yayathi; Johns, Dexton Antony; Vidyanath, S; Sam, George

    2013-05-01

    Periapical inflammatory lesion is the local response of bone around the apex of tooth that develops after the necrosis of the pulp tissue or extensive periodontal disease. The final outcome of the nature of wound healing after endodontic surgery can be repair or regeneration depending on the nature of the wound; the availability of progenitor cells; signaling molecules; and micro-environmental cues such as adhesion molecules, extracellular matrix, and associated non-collagenous protein molecules. The purpose of this case report is to add knowledge to the existing literature about the combined use of graft material [platelet rich fibrin (PRF) and hydroxyapatite (HA)] and barrier membrane in the treatment of large periapical lesion. A periapical endodontic surgery was performed on a 45 year old male patient with a swelling in the upper front teeth region and a large bony defect radiologically. The surgical defect was filled with a combination of PRF and HA bone graft crystals. The defect was covered by PRF membrane and sutured. Clinical examination revealed uneventful wound healing. Radiologically the HA crystals have been completely replaced by new bone at the end of 2 years. On the basis of the results obtained in our case report, we hypothesize that the use of PRF in conjunction with HA crystals might have accelerated the resorption of the graft crystals and would have induced the rapid rate of bone formation.

  15. Platelet Rich Fibrin in double lateral sliding bridge flap procedure for gingival recession coverage: An original study

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    Vijayalakshmi Rajaram

    2015-01-01

    Full Text Available Background: Gingival recession is a common occurrence in periodontal disease leading to an unaesthetic appearance of the gingiva. The effect of platelet-rich fibrin (PRF, when used along with double lateral sliding bridge flap (DLSBF, remains unknown. The aim of this study is to evaluate the effect of PRF in conjunction with DLSBF for multiple gingival recessions. Materials and Methods: Twenty systemically healthy individuals exhibiting Grade II gingival recession on their mandibular central incisors were recruited in this study. These patients were randomly assigned into two groups: DLSBF and PRF + DLSBF. The clinical parameters that were evaluated in this study were gingiva recession height, gingiva recession width, width of keratinized gingiva, clinical attachment level, and probing depth. PRF was procured from the patient's blood at the time of the surgery and used for the procedure. The follow-up was performed at 12 and 24 weeks postsurgery. Results: Statistically significant difference was observed between the clinical parameters at baseline and 12 and 24 weeks within the groups. There was no statistically significant difference, between the groups. Mean root coverage (RC was 80% ±29.1% in the DLSBF group and 78.8% ±37.6% in the DLSBF + PRF group with no statistically significant difference. Conclusion: From the results obtained in this study, the addition of PRF to DLSBF gives no additional benefits to the clinical parameters measured in RC.

  16. Platelet-rich fibrin as an adjunct to palatal wound healing after harvesting a free gingival graft: A case series

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    Mihir Raghavendra Kulkarni

    2014-01-01

    Full Text Available Background: Owing to its stimulatory effect on angiogenesis and epithelialization, platelet-rich fibrin (PRF is an excellent material for enhancing wound healing. The use of PRF dressings may be a simple and effective method of reducing the morbidity associated with donor sites of autogenous free gingival grafts (FGGs. The purpose of this case series is to document the beneficial role of PRF in the healing of FGG donor sites. Materials and Methods: A total of 18 patients treated with FGGs could be classified into two groups. PRF was prepared, compressed and used to dress the palatal wound followed by a periodontal pack in one group (10 patients and only a periodontal pack was used in the other group (8 patients. Post-operative healing was assessed clinically at 7, 14 and 21 days and the morbidity was assessed qualitatively by an interview. Results: Sites where PRF was used showed complete wound closure by 14 days and these patients reported lesser post-operative morbidity than patients in whom PRF was not used. Conclusions: PRF as a dressing is an effective method of enhancing the healing of the palatal donor site and consequently reducing the post-operative morbidity.

  17. Effect of centrifugation time on growth factor and MMP release of an experimental platelet-rich fibrin-type product.

    Science.gov (United States)

    Eren, Gülnihal; Gürkan, Ali; Atmaca, Harika; Dönmez, Ayhan; Atilla, Gül

    2016-07-01

    Platelet-rich fibrin (PRF) has a controlled release of growth factors due to the fibrin matrix structure. Different centrifugation protocols were suggested for PRF preparation. Since the derivation method of PRF can alter its contents, in the present study it is aimed to investigate the cell contents and transforming growth factor beta-1 (TGF-β1), platelet-derived growth factor (PDGF-AB), vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-1 and-8 release from experimental PRF-type membranes obtained with different centrifugation times at 400 gravity. Three blood samples were collected from 20 healthy non-smoker volunteers. One tube was used for whole blood analyses. The other two tubes were centrifuged at 400 g for 10 minutes (group A) or 12 minutes (group B). Each experimental PRF-type membrane was placed in Dulbecco's Modified Eagle's Medium (DMEM)and at 1, 24 and 72 hours, TGF-β1, PDGF-AB, VEGF, MMP-1 and -8 release amounts were analysed by enzyme-linked immunosorbent assay (ELISA). The blood cell count of membranes was determined by subtracting plasma supernatant and red blood cell (RBC) mixture from the whole blood cell counts. At 72 hours, the VEGF level of group B was statistically higher than that of group A (p = 0.040). The centrifugation time was not found to influence the release of other growth factors, enzymes and cell counts. Within the limits of the present study, it might be suggested that centrifugation time at a constant gravity has a significant effect on the VEGF levels released from experimental PRF-type membrane. It can be concluded that due to the importance of VEGF in the tissue healing process, membranes obtained at 12-minute centrifugation time may show a superior potential in wound healing. PMID:26830681

  18. Autologous Platelet-Rich Plasma for the Treatment of Pattern Hair Loss.

    Science.gov (United States)

    Singh, Babu; Goldberg, Lynne J

    2016-08-01

    Platelet-rich plasma (PRP) is a solution derived from whole blood that is enriched in the platelet fraction. Platelets serve as a reservoir of growth factors and cytokines. When platelets are activated in vivo, signaling molecules are released into the immediate microenvironment and activate receptors for various pathways. Historically, PRP has been applied to wound beds to promote healing of complex wounds. Over the last decade, it has served as a valuable therapeutic tool in various specialties such as maxillofacial surgery, plastic surgery, orthopedics and sports medicine. Only recently has PRP been utilized for dermatologic purposes, more specifically, for the treatment of male and female pattern hair loss. In this review, we discuss molecular and cellular pathways upregulated by PRP important in hair folliculogenesis, and examine clinical evidence from all previously published studies involving the use of PRP for pattern hair loss. PMID:27234711

  19. Updates of Choukroun's platelet-rich fibrin in dental implant bone defect%Choukroun富血小板纤维蛋白在口腔种植骨缺损中的研究与进展

    Institute of Scientific and Technical Information of China (English)

    许丰伟; 柳忠豪

    2012-01-01

    背景:近年来,Choukroun富血小板纤维蛋白作为一种富含自体细胞因子和生长因子的新型生物材料,在口腔种植骨缺损的临床应用中受到了广泛关注.目的:就富血小板纤维蛋白的实验及临床研究进展进行综述.方法:由第一作者应用计算机检索PubMed数据库及中国期刊网全文数据库2000-01/2011-12有关富血小板纤维蛋白生物学特性、实验研究以及临床应用等方面的文章,英文检索词为"choukroun's platelet-rich fibrin,platelet-rich plasma,dental implant,bone defect,GBR",中文检索词为"富血小板纤维蛋白,富血小板血浆,口腔种植,骨缺损,引导骨再生".排除重复性研究,共保留30篇文献进行综述.结果与结论:富血小板纤维蛋白由纤维蛋白网、血小板及粒细胞等组成,是目前较为先进的一种富含成骨作用因子的血液制品.具有制备过程操作简单、不需要添加其他制剂的特点,同时还有效模拟生理状态下血凝块中纤维蛋白的形成,与人体正常血凝块中的纤维蛋白相似,并很好地避免了免疫排斥和交叉感染的发生.在实验研究及临床应用中均显示了很好的促进组织愈合的能力.加之其成本低廉、取材方便等优点,在今后的口腔种植领域将得到越来越多的关注.%BACKGROUND: Recently, Choukroun's platelet-rich fibrin (PRF) is known as a rich source of autologous cytokines and growth factors, and special attention is paid as a new biomaterial in dental implant bone defect application.OBJECTIVE: To review experiments and clinical researches of the PRF.METHODS: A computer-based retrieval was performed by the first author in the CNKI and PubMed databases to search papers published between January 2000 and December 2011 with the key words of “Choukroun's platelet-rich fibrin, Platelet-rich plasma,dental implant, bone defect, GBR” in Chinese and English. According to inclusion and exclusion criteria, 30 papers were

  20. Platelet Rich Fibrin (P.R.F. in Reconstructive Surgery of Atrophied Maxillary Bones: Clinical and Histological Evaluations

    Directory of Open Access Journals (Sweden)

    Marco Tatullo, Massimo Marrelli, Michele Cassetta, Andrea Pacifici, Luigi Vito Stefanelli, Salvatore Scacco, Gianna Dipalma, Luciano Pacifici, Francesco Inchingolo

    2012-01-01

    Full Text Available Introduction. Maxillary bone losses often require additional regenerative procedures: as a supplement to the procedures of tissue regeneration, a platelet concentrate called PRF (Platelet Rich Fibrin was tested for the first time in France by Dr. Choukroun.Aim of the present study is to investigate, clinically and histologically, the potential use of PRF, associated with deproteinized bovine bone (Bio-Oss, as grafting materials in pre-implantology sinus grafting of severe maxillary atrophy, in comparison with a control group, in which only deproteinized bovine bone (Bio-Oss was used as reconstructive material.Materials and Methods. 60 patients were recruited using the cluster-sampling method; inclusion criteria were maxillary atrophy with residual ridge < 5mm. The major atrophies in selected patients involved sinus-lift, with a second-look reopening for the implant insertion phase. The used grafting materials were: a Bio-Oss and b amorphous and membranous PRF together with Bio-Oss. We performed all operations by means of piezosurgery in order to reduce trauma and to optimize the design of the operculum on the cortical bone. The reopening of the surgical area was scheduled at 3 different times.Results. 72 sinus lifts were performed with subsequent implants insertions.We want to underline how the histological results proved that the samples collected after 106 days (Early protocol with the adding of PRF were constituted by lamellar bone tissue with an interposed stroma that appeared relaxed and richly vascularized.Conclusions. The use of PRF and piezosurgery reduced the healing time, compared to the 150 days described in literature, favoring optimal bone regeneration. At 106 days, it is already possible to achieve good primary stability of endosseous implants, though lacking of functional loading.

  1. Effect of platelet-rich plasma and fibrin glue on healing of critical-size calvarial bone defects.

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    Findikcioglu, Kemal; Findikcioglu, Fulya; Yavuzer, Reha; Elmas, Cigdem; Atabay, Kenan

    2009-01-01

    Despite the insufficient number of experimental studies, platelet-rich plasma (PRP) including high amounts of growth factors is introduced to clinical use rapidly. The aim of this study was to compare the effects of PRP and platelet-poor plasma (PPP) on healing of critical-size bone defects.Bilateral full-thickness, critical-size bone defects were created in the parietal bones of 32 rabbits, which had been studied in 4 groups. Saline, thrombin solution, PPP, and PRP were applied to the created defects before closure. Radiologic defect area measurement results at 0, 4, and 16 weeks were compared between the groups. In addition, densities of the newly formed bones at 16th week were studied. Histologic parameters (primary and secondary bone trabecula, neovascularization, and bone marrow and connective tissue formation) were compared between 4- and 16-week groups.More rapid decrease in defect size was observed in groups 3 and 4 than in groups 1 and 2, both in the 4th and 16th weeks. Newly formed bone densities were also found to be higher in these 2 groups. New bone formation was detected to be more rapid considering histologic parameters, in groups 3 and 4 at 4th and 16th weeks.Study demonstrates that PRP and PPP might have favorable effects on bone healing. Although we cannot reveal any statistical difference between these 2 substances considering osteoinductive potential, PRP group has demonstrated superior results compared with fibrin glue group. Higher platelet concentrations may expose beneficial effects of PRP.

  2. Effects of Plasma Rich in Growth Factors and Platelet-Rich Fibrin on Proliferation and Viability of Human Gingival Fibroblasts

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    Surena Vahabi

    2016-01-01

    Full Text Available Objectives: Platelet preparations are commonly used to enhance bone and soft tissue regeneration. Considering the existing controversies on the efficacy of platelet products for tissue regeneration, more in vitro studies are required. The aim of the present study was to compare the in vitro effects of plasma rich in growth factors (PRGF and platelet-rich fibrin (PRF on proliferation and viability of human gingival fibroblasts (HGFs.Materials and Methods: Anitua's PRGF and Choukran's PRF were prepared according to the standard protocols. After culture periods of 24, 48 and 72 hours, proliferation of HGFs was evaluated by the methyl thiazol tetrazolium assay. Statistical analysis was performed using one-way ANOVA followed by Tukey-Kramer’s multiple comparisons and P-values<0.05 were considered statistically significant.Results: PRGF treatment induced statistically significant (P<0.001 proliferation of HGF cells compared to the negative control (100% viability at 24, 48 and 72 hours in values of 123%±2.25%, 102%±2.8% and 101%±3.92%, respectively. The PRF membrane treatment of HGF cells had a statistically significant effect on cell proliferation (21%±1.73%, P<0.001 at 24 hours compared to the negative control. However, at 48 and 72 hours after treatment, PRF had a negative effect on HGF cell proliferation and caused 38% and 60% decrease in viability and proliferation compared to the negative control, respectively. The HGF cell proliferation was significantly higher in PRGF than in PRF group (P< 0.001.Conclusion: This study demonstrated that PRGF had a strong stimulatory effect on HGF cell viability and proliferation compared to PRF.

  3. Simultaneous implant placement and bone regeneration around dental implants using tissue-engineered bone with fibrin glue, mesenchymal stem cells and platelet-rich plasma.

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    Ito, Kenji; Yamada, Yoichi; Naiki, Takahito; Ueda, Minoru

    2006-10-01

    This study was undertaken to evaluate the use of tissue-engineered bone as grafting material for alveolar augmentation with simultaneous implant placement. Twelve adult hybrid dogs were used in this study. One month after the extraction of teeth in the mandible region, bone defects on both sides of the mandible were induced using a trephine bar with a diameter of 10 mm. Dog mesenchymal stem cells (dMSCs) were obtained via iliac bone biopsy and cultured for 4 weeks before implantation. After installing the dental implants, the defects were simultaneously implanted with the following graft materials: (i) fibrin, (ii) dMSCs and fibrin (dMSCs/fibrin), (iii) dMSCs, platelet-rich plasma (PRP) and fibrin (dMSCs/PRP/fibrin) and (iv) control (defect only). The implants were assessed by histological and histomorphometric analysis, 2, 4 and 8 weeks after implantation. The implants exhibited varying degrees of bone-implant contact (BIC). The BIC was 17%, 19% and 29% (control), 20%, 22% and 25% (fibrin), 22%, 32% and 42% (dMSCs/fibrin) and 25%, 49% and 53% (dMSCs/PRP/fibrin) after 2, 4 and 8 weeks, respectively. This study suggests that tissue-engineered bone may be of sufficient quality for predictable enhancement of bone regeneration around dental implants when used simultaneous by with implant placement.

  4. Efficacy of autologous platelet-rich plasma in the treatment of chronic nonhealing leg ulcers

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    Shwetha Suryanarayan

    2014-09-01

    Full Text Available Aim: The aim was to evaluate the efficacy of platelet-rich plasma (PRP in the treatment of chronic nonhealing ulcers. Methods: A nonrandomized, uncontrolled study was performed on 24 patients with 33 nonhealing ulcers of various etiologies. All patients were treated with PRP at weekly intervals for a maximum of 6 treatments. At the end of the 6-week period, reduction in size of the ulcers (area and volume was assessed. Results: The mean age of the patients was 42.5 years (standard deviation [SD] 12.48. Of 33 ulcers, there were 19 venous ulcers, 7 traumatic ulcers, 2 ulcers secondary to pyoderma gangrenosum, 2 diabetic ulcers, 2 trophic ulcers, and 1 vasculitic ulcer. The mean duration of healing of the ulcers was 5.6 weeks (SD 3.23. The mean percentage of reduction in area and volume of the ulcers was 91.7% (SD 18.4% and 95% (SD 14%, respectively. About 100% resolution in the area was seen in 25 (76% of the ulcers and 100% reduction in volume was seen in 24 (73% of the ulcers at the end of the 6th treatment. Conclusion: Conventional therapies do not provide satisfactory healing for chronic nonhealing ulcers as they are not able to provide the necessary growth factors (GFs (platelet-derived GF, epidermal GF, vascular endothelial GF, etc. which are essential for the healing process. PRP is a safe, affordable, biocompatible, and simple office-based procedure for the treatment of nonhealing ulcers.

  5. Use of second-generation platelet concentrate (platelet-rich fibrin and hydroxyapatite in the management of large periapical inflammatory lesion: A computed tomography scan analysis

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    Hemalatha Hiremath

    2014-01-01

    Full Text Available Periapical surgery is required when periradicular pathosis associated with endodontically treated teeth cannot be resolved by nonsurgical root canal therapy (retreatment, or when retreatment was unsuccessful, not feasible or contraindicated. Endodontic failures can occur when irritants remain within the confines of the root canal, or when an extraradicular infection cannot be eradicated by orthograde root canal treatment. Foreign-body responses toward filling materials, toward cholesterol crystals or radicular cysts, might prevent complete periapical healing. We present here a case report wherein, combination of platelet-rich fibrin (PRF and the hydroxyapatite graft was used to achieve faster healing of the large periapical lesion. Healing was observed within 8 months, which were confirmed by computed tomography, following improved bone density. PRF has many advantages over platelet-rich plasma. It provides a physiologic architecture that is very favorable to the healing process, which is obtained due to the slow polymerization process.

  6. Use of second-generation platelet concentrate (platelet-rich fibrin) and hydroxyapatite in the management of large periapical inflammatory lesion: a computed tomography scan analysis.

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    Hiremath, Hemalatha; Motiwala, Tejas; Jain, Pradeep; Kulkarni, Sadanand

    2014-01-01

    Periapical surgery is required when periradicular pathosis associated with endodontically treated teeth cannot be resolved by nonsurgical root canal therapy (retreatment), or when retreatment was unsuccessful, not feasible or contraindicated. Endodontic failures can occur when irritants remain within the confines of the root canal, or when an extraradicular infection cannot be eradicated by orthograde root canal treatment. Foreign-body responses toward filling materials, toward cholesterol crystals or radicular cysts, might prevent complete periapical healing. We present here a case report wherein, combination of platelet-rich fibrin (PRF) and the hydroxyapatite graft was used to achieve faster healing of the large periapical lesion. Healing was observed within 8 months, which were confirmed by computed tomography, following improved bone density. PRF has many advantages over platelet-rich plasma. It provides a physiologic architecture that is very favorable to the healing process, which is obtained due to the slow polymerization process.

  7. Current knowledge and perspectives for the use of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in oral and maxillofacial surgery part 2: Bone graft, implant and reconstructive surgery.

    Science.gov (United States)

    Simonpieri, Alain; Del Corso, Marco; Vervelle, Alain; Jimbo, Ryo; Inchingolo, Francesco; Sammartino, Gilberto; Dohan Ehrenfest, David M

    2012-06-01

    Platelet concentrates for surgical use are innovative tools of regenerative medicine, and were widely tested in oral and maxillofacial surgery. Unfortunately, the literature on the topic is contradictory and the published data are difficult to sort and interpret. In bone graft, implant and reconstructive surgery, the literature is particularly dense about the use of the various forms of Platelet-Rich Plasma (PRP) - Pure Platelet-Rich Plasma (P-PRP) or Leukocyte- and Platelet-Rich Plasma (L-PRP) - but still limited about Platelet-Rich Fibrin (PRF) subfamilies. In this second article, we describe and discuss the current published knowledge about the use of PRP and PRF during implant placement (particularly as surface treatment for the stimulation of osseointegration), the treatment of peri-implant bone defects (after peri-implantitis, during implantation in an insufficient bone volume or during immediate post-extraction or post-avulsion implantation), the sinuslift procedures and various complex implant-supported treatments. Other potential applications of the platelet concentrates are also highlighted in maxillofacial reconstructive surgery, for the treatment of patients using bisphosphonates, anticoagulants or with post-tumoral irradiated maxilla. Finally, we particularly insist on the perspectives in this field, through the description and illustration of the use of L-PRF (Leukocyte- and Platelet-Rich Fibrin) clots and membranes during the regeneration of peri-implant bone defects, during the sinus-lift procedure and during complex implant-supported rehabilitations. The use of L-PRF allowed to define a new therapeutic concept called the Natural Bone Regeneration (NBR) for the reconstruction of the alveolar ridges at the gingival and bone levels. As it is illustrated in this article, the NBR principles allow to push away some technical limits of global implant-supported rehabilitations, particularly when combined with other powerful biotechnological tools

  8. Effects of Autologous Platelets Rich Plasma on Full-thickness Cutaneous Wounds Healing in Goats

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    A.H. AL-Bayati

    2013-12-01

    Full Text Available This investigation was designed to evaluate the role of Platelet-Rich Plasma (PRP on healing of experimentally wounded skin in ten adult bucks, aged 2-3 years and weighing 25-30 kg. The animals divided randomly and equally into (control and treatment groups. Four of 3×3 cm of full-thickness square cutaneous wounds was induced on both sides of the lateral thoracic region of each animal under the effect of local anesthetic proceeding by xylazine hydrochloride as a sedative. A pair of left wounds was treated by injection with 5 mL of autonomous PRP (treatment group, 2 mm lateral to the wound edges and in the wound center. While, the right wound were injected by 5 mL of sterile saline by the same procedure (control group. Each group was divided into five subgroups (four wounds of each, for morph metrical and histopathological evaluations of wound healing process represented by percent of wound contraction, epithelialization and total healing at 3, 7, 14, 21 and 28 days post-wounding. The morphometrical appearance of the wounds which treated with PRP, showed that the contraction, re-epithelialization and healing percent were statically significant (p<0.05 in comparison with control wounds during four weeks study. Based on histopathological results, there was re-epithelialization of epidermis, with highly cellular granulation tissue, well differentiated keratinocytes of epidermis with scar formation in the dermis of the sectioned skin. We conclude that local injection of PRP leads to accelerate and improvement of wound healing in comparison to control wounds.

  9. Clinical application of platelet-rich fibrin by the application of the Double J technique during implant placement in alveolar bone defect areas: case reports.

    Science.gov (United States)

    Kim, Jin-Son; Jeong, Moon-Hwan; Jo, Ji-Ho; Kim, Su-Gwan; Oh, Ji-Su

    2013-06-01

    Platelet-rich fibrin (PRF) belongs to a new generation of platelet concentrates, with simplified processing and without biochemical blood handling. PRF releases growth factors and matrix glycoproteins. In this study, the Double J technique was used. The Double J technique, which uses centrifuged venous blood that is sampled using 2 different types of DB vacutainers, is a procedure that covers the PRF matrix obtained from 1 of the DB vacutainers on transplanted osseous coagulum, which is obtained using the plasma layer and buffering layer from the second DB vacutainer. Two cases were reported because clinically valid results were obtained. Additional studies are definitely warranted.

  10. A split mouth randomized controlled study to evaluate the adjunctive effect of platelet-rich fibrin to coronally advanced flap in Miller′s class-I and II recession defects

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    Rajan Padma

    2013-01-01

    Full Text Available Background: There are various techniques developed to treat the exposed roots, a recent innovation in dentistry is the use of second generation platelet concentrate which is an autologous platelet-rich fibrin gel (PRF with growth factors and cicatricial properties for root coverage procedures. Therefore, the present research was undertaken to study the additional benefits of PRF when used along with coronally advanced flap (CAF. Materials and Methods: Total of 15 systemically healthy subjects presenting bilateral isolated Miller′s class I and II recession were enrolled into the study. Each patient was randomly treated with a combination of CAF along with a platelet-rich fibrin (PRF membrane on the test site and CAF alone on the control site. Recession depth, clinical attachment level (CAL, and width of keratinized gingiva (WKG were compared with baseline at 1, 3, and 6 months between test and control sites. Results: Mean percentage root coverage in the test group after 1, 3, and 6 months was 34.58, 70.73, and 100, respectively. Differences between the control and test groups were statistically significant. This study also showed a statistically significant increase in WKG in the test group (2.94 ± 0.77 at baseline to 5.38 ± 1.67 at 6 months. Conclusion: CAF is a predictable treatment for isolated Miller′s class I and II recession defects. The addition of PRF membrane with CAF provides superior root coverage with additional benefits of gain in CAL and WKG at 6 months postoperatively.

  11. 复合PRP的可注射型组织工程骨修复兔桡骨缺损的实验研究%Injectable tissue-engineered bone made of autologous platelet-rich plasma, fibrin glue and BMSCs for repairing segmental radial defect in rabbits

    Institute of Scientific and Technical Information of China (English)

    黄爱文; 金丹; 裴国献; 曾宪利; 胡稷杰; 林海宁

    2007-01-01

    目的 以自体富血小板血浆(platelet-rich plasma, PRP)、骨髓基质干细胞(bone marrow stromal cells, BMSCs)和纤维蛋白胶构建可注射型组织工程骨并探讨其修复骨缺损的效果.方法 36只新西兰兔分为A、B、C 3组,每组12只.A、B组动物术前4 h抽取耳背中央动脉血提取PRP. A组于术前1~2个月抽取双髂骨处骨髓并培养出BMSCs,在体外与纤维蛋白胶(FG)及自体PRP构建成可注型组织工程骨,植入自体桡骨1.5 cm节段性骨缺损,为实验组.B、C两组分别植入PRP+FG、FG于同样骨缺损处为对照组.另取4只桡骨同样部位和大小骨缺损旷置作为空白组.术后观察其一般情况并于4、8、12周取材做组织学切片,术后12周取尺桡骨做生物力学测试.分别从组织学观察、生物力学方面评估比较骨缺损的修复情况.结果 组织学观察见A、B组各时间点新骨形成均优于C组.生物力学比较:12周时A组桡骨生物力学强度与正常桡骨比较无明显差异(P>0.05),但其明显优于B组(P<0.05).结论 PRP对骨缺损愈合有明显促进作用,复合PRP的可注射型骨修复材料及构建的含种子细胞的组织工程骨均可修复节段性骨缺损,但种子细胞的添加可明显促进新骨成熟度和增强其生物力学性能.

  12. Symptomatic knee osteoarthritis treatment using autologous adipose derived stem cells and platelet-rich plasma: a clinical study

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    Phuc Van Pham

    2014-01-01

    Full Text Available Osteoarthritis is one of the most common diseases, and it affects 12% of the population around the world. Although the disease is chronic, it significantly reduces the patient's quality of life. At present, stem cell therapy is considered to be an efficient approach for treating this condition. Mesenchymal stem cells (MSCs show the most potential for stem cell therapy of osteoarthritis. In fact, MSCs can differentiate into certain mesodermal tissues such as cartilage and bone. Therefore, in the present study, we applied adipose tissue-derived MSCs to osteoarthritis treatment. This study aimed to evaluate the clinical efficiency of autologous adipose tissue-derived MSC transplantation in patients with confirmed osteoarthritis at grade II and III. Adipose tissue was isolated from the belly, and used for extraction of the stromal vascular fraction (SVF. The SVF was mixed with activated platelet- rich plasma before injection. The clinical efficiencies were evaluated by the pain score (VAS, Lysholm score, and MRI findings. We performed the procedure in 21 cases from 2012 to 2013. All 21 patients showed improved joint function after 8.5 months. The pain score decreased from 7.6+/-0.5 before injection to 3.5+/-0.7 at 3 months and 1.5+/-0.5 at 6 months after injection. The Lysholm score increased from 61+/-11 before injection to 82+/-8.1 after injection. Significant improvements were noted in MRI findings, with increased thickness of the cartilage layer. Moreover, there were no side-effects or complications related to microorganism infection, graft rejection, or tumorigenesis. These results provide a new opportunity for osteoarthritis treatment. Level of evidence: IV. [Biomed Res Ther 2014; 1(1.000: 02-08

  13. Platelet-rich plasma gel in combination with Schwann cells for repair of sciatic nerve injury

    Institute of Scientific and Technical Information of China (English)

    Fagang Ye; Haiyan Li; Guangxi Qiao; Feng Chen; Hao Tao; Aiyu Ji; Yanling Hu

    2012-01-01

    Bone marrow mesenchymal stem cells were isolated from New Zealand white rabbits,culture-expanded and differentiated into Schwann cell-like cells.Autologous platelet-rich plasma and Schwann cell-like cells were mixed in suspension at a density of 1 × 106 cells/mL,prior to introduction into a poly (lactic-co-glycolic acid) conduit.Fabricated tissue-engineered nerves were implanted into rabbits to bridge 10 mm sciatic nerve defects (platelet-rich plasma group).Controls were established using fibrin as the seeding matrix for Schwann cell-like cells at identical density to construct tissue-engineered nerves (fibrin group).Twelve weeks after implantation,toluidine blue staining and scanning electron microscopy were used to demonstrate an increase in the number of regenerating nerve fibers and thickness of the myelin sheath in the platelet-rich plasma group compared with the fibrin group.Fluoro-gold retrograde labeling revealed that the number of Fluoro-gold-positive neurons in the dorsal root ganglion and the spinal cord anterior horn was greater in the platelet-rich plasma group than in the fibrin group.Electrophysiological examination confirmed that compound muscle action potential and nerve conduction velocity were superior in the platelet-rich plasma group compared with the fibrin group.These results indicate that autologous platelet-rich plasma gel can effectively serve as a seeding matrix for Schwann cell-like cells to construct tissue-engineered nerves to promote peripheral nerve regeneration.

  14. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part III: leucocyte activation: a new feature for platelet concentrates?

    Science.gov (United States)

    Dohan, David M; Choukroun, Joseph; Diss, Antoine; Dohan, Steve L; Dohan, Anthony J J; Mouhyi, Jaafar; Gogly, Bruno

    2006-03-01

    Platelet-rich fibrin (PRF) belongs to a new generation of platelet concentrates, with simplified processing and without biochemical blood handling. In this third article, we investigate the immune features of this biomaterial. During PRF processing, leucocytes could also secrete cytokines in reaction to the hemostatic and inflammatory phenomena artificially induced in the centrifuged tube. We therefore undertook to quantify 5 significant cell mediators within platelet poor plasma supernatant and PRF clot exudate serum: 3 proinflammatory cytokines (IL-1beta, IL-6, and TNF-alpha), an antiinflammatory cytokine (IL-4), and a key growth promoter of angiogenesis (VEGF). Our data are correlated with that obtained in plasma (nonactivated blood) and in sera (activated blood). These initial analyses revealed that PRF could be an immune regulation node with inflammation retrocontrol abilities. This concept could explain the reduction of postoperative infections when PRF is used as surgical additive.

  15. Amalgamation of allogenic bone graft, platelet-rich fibrin gel, and PRF membrane in auto-transplantation of an impacted central incisor.

    Science.gov (United States)

    Chaudhary, Zainab; Kumar, Yuvika Raj; Mohanty, Sujata; Khetrapal, Ambica

    2015-01-01

    "Social six" teeth refers to the maxillary incisors and canines that play a vital role in the appearance of an individual and absence of any one of them has a significant psycho-social impact. Hence, early treatment and rehabilitation of the same are extremely important. A multitude of treatment options ranging from orthodontic extrusion, extraction followed by implant placement, fixed partial denture, and auto-transplantation have been advocated. This case report discusses the unique amalgamation of platelet-rich fibrin (PRF), demineralized freeze-dried bone graft with use of PRF membrane during auto-transplantation of an impacted central incisor. The authors have focused on maximum usage of autogenous materials in the most economic and least invasive manner. Furthermore, this amalgamation has been used to provide rehabilitation in the least span of time.

  16. Amalgamation of allogenic bone graft, platelet-rich fibrin gel, and PRF membrane in auto-transplantation of an impacted central incisor

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    Zainab Chaudhary

    2015-01-01

    Full Text Available "Social six" teeth refers to the maxillary incisors and canines that play a vital role in the appearance of an individual and absence of any one of them has a significant psycho-social impact. Hence, early treatment and rehabilitation of the same are extremely important. A multitude of treatment options ranging from orthodontic extrusion, extraction followed by implant placement, fixed partial denture, and auto-transplantation have been advocated. This case report discusses the unique amalgamation of platelet-rich fibrin (PRF, demineralized freeze-dried bone graft with use of PRF membrane during auto-transplantation of an impacted central incisor. The authors have focused on maximum usage of autogenous materials in the most economic and least invasive manner. Furthermore, this amalgamation has been used to provide rehabilitation in the least span of time.

  17. Surgical re-entry evaluation of regenerative efficacy of bioactive Gengigel™ and platelet-rich fibrin in the treatment of grade II furcation: A novel approach

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    Gurkirat Kaur Sandhu

    2015-01-01

    Full Text Available The furcation area creates situations in which routine periodontal procedures are somewhat limited, and surgical procedures are generally required. The introduction of bioactive agents, such as platelet concentrates, enamel matrix derivatives, bone morphogenic proteins, and matrix macromolecules such as hyaluronic acid has expanded the scope for better outcomes in furcation treatment. Hyaluronic acid is a naturally occurring nonsulfated high molecular weight glycosaminoglycan that forms a critical component of the extracellular matrix and contributes significantly to tissue hydrodynamics, cell migration, and proliferation. Platelet-rich fibrin (PRF is an immune and platelet concentrate containing all the constituents of a blood sample, which are favorable for healing and immunity. The purpose of the present case report was to assess through surgical re-entry, the regenerative capacity of Gengigel™ in conjunction with PRF in a patient with grade II furcation defect. It was observed that the combined approach resulted in significant furcation defect fill on re-evaluation at 6 months.

  18. Surgical re-entry evaluation of regenerative efficacy of bioactive Gengigel® and platelet-rich fibrin in the treatment of grade II furcation: A novel approach

    Science.gov (United States)

    Sandhu, Gurkirat Kaur; Khinda, Paramjit Kaur; Gill, Amarjit Singh; Kalra, Harveen Singh

    2015-01-01

    The furcation area creates situations in which routine periodontal procedures are somewhat limited, and surgical procedures are generally required. The introduction of bioactive agents, such as platelet concentrates, enamel matrix derivatives, bone morphogenic proteins, and matrix macromolecules such as hyaluronic acid has expanded the scope for better outcomes in furcation treatment. Hyaluronic acid is a naturally occurring nonsulfated high molecular weight glycosaminoglycan that forms a critical component of the extracellular matrix and contributes significantly to tissue hydrodynamics, cell migration, and proliferation. Platelet-rich fibrin (PRF) is an immune and platelet concentrate containing all the constituents of a blood sample, which are favorable for healing and immunity. The purpose of the present case report was to assess through surgical re-entry, the regenerative capacity of Gengigel® in conjunction with PRF in a patient with grade II furcation defect. It was observed that the combined approach resulted in significant furcation defect fill on re-evaluation at 6 months. PMID:26681869

  19. Surgical re-entry evaluation of regenerative efficacy of bioactive Gengigel(®) and platelet-rich fibrin in the treatment of grade II furcation: A novel approach.

    Science.gov (United States)

    Sandhu, Gurkirat Kaur; Khinda, Paramjit Kaur; Gill, Amarjit Singh; Kalra, Harveen Singh

    2015-01-01

    The furcation area creates situations in which routine periodontal procedures are somewhat limited, and surgical procedures are generally required. The introduction of bioactive agents, such as platelet concentrates, enamel matrix derivatives, bone morphogenic proteins, and matrix macromolecules such as hyaluronic acid has expanded the scope for better outcomes in furcation treatment. Hyaluronic acid is a naturally occurring nonsulfated high molecular weight glycosaminoglycan that forms a critical component of the extracellular matrix and contributes significantly to tissue hydrodynamics, cell migration, and proliferation. Platelet-rich fibrin (PRF) is an immune and platelet concentrate containing all the constituents of a blood sample, which are favorable for healing and immunity. The purpose of the present case report was to assess through surgical re-entry, the regenerative capacity of Gengigel(®) in conjunction with PRF in a patient with grade II furcation defect. It was observed that the combined approach resulted in significant furcation defect fill on re-evaluation at 6 months. PMID:26681869

  20. The adjuvant use of stromal vascular fraction and platelet-rich fibrin for autologous adipose tissue transplantation%可注射自体脂肪颗粒复合PRF和S VF促进移植脂肪组织再生

    Institute of Scientific and Technical Information of China (English)

    谭新颖; 刘斌; 刘彦普; 李龙; 徐海燕; 安然

    2014-01-01

    目的:探讨PRF和SVF对自体脂肪颗粒移植的作用,并分析这种可注射复合生物材料的移植效果.方法:健康新西兰家兔24只,分为四组:单纯脂肪组织移植组(2 mL AG+0.2 mL NS)、脂肪颗粒复合富血小板纤维蛋白(2 mL AG+0.2 mL PRF)组、脂肪颗粒复合基质血管成分(2 mL AG+0.2 mL SVF)组和脂肪颗粒复合PRF和SVF[2 mL AG+0.2 mL (SVF+PRF)]组.术后1、3、6月取材,进行大体观察、组织病理学检测、游标卡尺和B超对移植脂肪组织的体积测量.采用SPSS16.0软件,对四组移植脂肪组织的存活率及吸收率进行方差分析并采用LSD法对不同时间点组间进行比较.结果:24周后各组植入脂肪组织的吸收率分别为 AG +NS [(49.4±9.5)%],AG+SVF [(27.2±4.4)%],AG+PRF [(36.4±8.5)%]和AG+SVF+PRF [(17.4±6.2)%],并有显著差异(P<0.01).结论:实验结果表明自体脂肪组织复合PRF和SVF能够促进移植脂肪组织的再生,为临床脂肪组织移植提供一种新的策略.%AIM:This study was to explore the role of PRF and SVF on autologous adipose granule transplant,and analyze the effect of the transplantation of the injectable compound biological material.METHODS:24 healthy New Zealand rabbits were di-vided into four groups:2 ml adipose granules and 0.2 ml normal saline solution (AG+NS group),2 ml adipose granules and 0.2 ml SVF (AG+SVF group),2 ml adipose granules and 0.2 ml PRF (AG+PRF group)or 2 ml adipose granules combined with 0.1 ml SVF and 0.1 ml PRF (AG+SVF+PRF group).1,3,6 months after the operation,each group was taken gross observa-tion,histological examination,and was measured the volume of transplanted adipose tissue by Vernier caliper and B ultrasound. All data collected were expressed as means ±standard deviation (SD).Statistical analysis was performed by One-Way ANOVA and LSD method using SPSS 16

  1. Endodontic management of nonvital permanent teeth having immature roots with one step apexification, using mineral trioxide aggregate apical plug and autogenous platelet-rich fibrin membrane as an internal matrix: Case series.

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    Sharma, Vivek; Sharma, Sarang; Dudeja, Pooja; Grover, Shibani

    2016-01-01

    A tooth with blunderbuss canal and open apex can be an endodontic challenge because of difficulty in obtaining an apical seal, and existing thin radicular walls which are susceptible to fracture. To overcome the limitations of traditional long-term calcium hydroxide apexification procedures, nonsurgical one step apexification using an array of materials such as mineral trioxide aggregate (MTA) has been suggested. However, adequate compaction of MTA in teeth with wide open apices can be an arduous task, and an internal matrix is required for controlled placement of MTA against which obturating material can be condensed. Platelet-rich fibrin (PRF), a second generation platelet concentrate containing several growth factors that promotes hard and soft-tissue healing, has been used as an internal matrix to create an apical plug of MTA and hence prevent extrusion of filling materials. This case series presents the endodontic management of immature permanent teeth with open apices using internal matrix of autologous PRF membrane and one step apical barrier placement of MTA. PMID:27041904

  2. Platelet-rich fibrin for repair of oral soft tissue defects%富血小板纤维蛋白诱导口腔缺损软组织的修复与再生

    Institute of Scientific and Technical Information of China (English)

    王拓; 杨琴秋; 董露; 肖琼; 陈红亮; 孙勇; 钟科

    2016-01-01

    BACKGROUND:Insufficient oral soft tissues in the implant zone may have a negative effect on the wound healing and the aesthetic restoration in the late stage. Platelet-rich fibrin can promote the wound healing of soft tissue defects. But there is stil a lack of in-depth studies on the promotion of oral soft tissue defects in animal experiments. OBJECTIVE: To compare the repairing effects of platelet-rich fibrin and colagen membrane on soft tissue defects of the hard palate in New Zealand rabbits. METHODS:Fifty-four New Zealand rabbits were randomly divided into three groups (n=14 per group): platelet-rich fibrin group, colagen membrane group and blank control group. A 5 mm-diameter circular ful-thickness soft tissue defect was made in the front of the hard palate, 2 mm distant to the rear maxilary incisors and mucosal edge of the bilateral hard palates. Autologous platelet-rich fibrin membrane or colagen membrane were implanted into the defect in the platelet-rich fibrin group and colagen membrane group, respectively. No treatment was given in the blank control group. General observation of the wound and wound healing analysis were performed at days 3, 7, 14, 21, 28, 56 post operation. Hematoxylin-eosin staining, CD31 immunohistochemical staining and Masson staining were used to observe inflammatory reaction, angiogenesis and colagen formation in the surgical site. RESULTS AND CONCLUSION:The wound healing rate was fastest in the platelet-rich fibrin group, and no obvious scar formed. At 3 days post operation, there was no difference in the wound healing rates among the three groups; at 7 days, the wound healing rate in the platelet-rich fibrin group was significantly higher than that in the colagen membrane group and blank control group (P   目的:对比观察富血小板纤维蛋白和胶原生物膜修复新西兰兔硬腭软组织缺损的效果。  方法:将54只新西兰兔随机分为3组,分别为富血小板纤维蛋白组、胶原膜

  3. The heat-compression technique for the conversion of platelet-rich fibrin preparation to a barrier membrane with a reduced rate of biodegradation.

    Science.gov (United States)

    Kawase, Tomoyuki; Kamiya, Mana; Kobayashi, Mito; Tanaka, Takaaki; Okuda, Kazuhiro; Wolff, Larry F; Yoshie, Hiromasa

    2015-05-01

    Platelet-rich fibrin (PRF) was developed as an advanced form of platelet-rich plasma to eliminate xenofactors, such as bovine thrombin, and it is mainly used as a source of growth factor for tissue regeneration. Furthermore, although a minor application, PRF in a compressed membrane-like form has also been used as a substitute for commercially available barrier membranes in guided-tissue regeneration (GTR) treatment. However, the PRF membrane is resorbed within 2 weeks or less at implantation sites; therefore, it can barely maintain sufficient space for bone regeneration. In this study, we developed and optimized a heat-compression technique and tested the feasibility of the resulting PRF membrane. Freshly prepared human PRF was first compressed with dry gauze and subsequently with a hot iron. Biodegradability was microscopically examined in vitro by treatment with plasmin at 37°C or in vivo by subcutaneous implantation in nude mice. Compared with the control gauze-compressed PRF, the heat-compressed PRF appeared plasmin-resistant and remained stable for longer than 10 days in vitro. Additionally, in animal implantation studies, the heat-compressed PRF was observed at least for 3 weeks postimplantation in vivo whereas the control PRF was completely resorbed within 2 weeks. Therefore, these findings suggest that the heat-compression technique reduces the rate of biodegradation of the PRF membrane without sacrificing its biocompatibility and that the heat-compressed PRF membrane easily could be prepared at chair-side and applied as a barrier membrane in the GTR treatment.

  4. Effect of Platelet-Rich Plasma (PRP versus Autologous Whole Blood on Pain and Function Improvement in Tennis Elbow: A Randomized Clinical Trial

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    Seyed Ahmad Raeissadat

    2014-01-01

    Full Text Available Background. Autologous whole blood and platelet-rich plasma (PRP have been both suggested to treat chronic tennis elbow. The aim of the present study was to compare the effects of PRP versus autologous whole blood local injection in chronic tennis elbow. Methods. Forty patients with tennis elbow were randomly divided into 2 groups. Group 1 was treated with a single injection of 2 mL of autologous PRP and group 2 with 2 mL of autologous blood. Tennis elbow strap, stretching, and strengthening exercises were administered for both groups during a 2-month followup. Pain and functional improvements were assessed using visual analog scale (VAS, modified Mayo Clinic performance index for the elbow, and pressure pain threshold (PPT at 0, 4, and 8 weeks. Results. All pain and functional variables including VAS, PPT, and Mayo scores improved significantly in both groups 4 weeks after injection. No statistically significant difference was noted between groups regarding pain scores in 4-week follow-up examination (P>0.05. At 8-week reevaluations, VAS and Mayo scores improved only in PRP group (P<0.05. Conclusion. PRP and autologous whole blood injections are both effective to treat chronic lateral epicondylitis. PRP might be slightly superior in 8-week followup. However, further studies are suggested to get definite conclusion.

  5. Effect of Platelet-Rich Plasma (PRP) versus Autologous Whole Blood on Pain and Function Improvement in Tennis Elbow: A Randomized Clinical Trial

    Science.gov (United States)

    Raeissadat, Seyed Ahmad; Sedighipour, Leyla; Rayegani, Seyed Mansoor; Bahrami, Mohammad Hasan; Bayat, Masume; Rahimi, Rosa

    2014-01-01

    Background. Autologous whole blood and platelet-rich plasma (PRP) have been both suggested to treat chronic tennis elbow. The aim of the present study was to compare the effects of PRP versus autologous whole blood local injection in chronic tennis elbow. Methods. Forty patients with tennis elbow were randomly divided into 2 groups. Group 1 was treated with a single injection of 2 mL of autologous PRP and group 2 with 2 mL of autologous blood. Tennis elbow strap, stretching, and strengthening exercises were administered for both groups during a 2-month followup. Pain and functional improvements were assessed using visual analog scale (VAS), modified Mayo Clinic performance index for the elbow, and pressure pain threshold (PPT) at 0, 4, and 8 weeks. Results. All pain and functional variables including VAS, PPT, and Mayo scores improved significantly in both groups 4 weeks after injection. No statistically significant difference was noted between groups regarding pain scores in 4-week follow-up examination (P > 0.05). At 8-week reevaluations, VAS and Mayo scores improved only in PRP group (P < 0.05). Conclusion. PRP and autologous whole blood injections are both effective to treat chronic lateral epicondylitis. PRP might be slightly superior in 8-week followup. However, further studies are suggested to get definite conclusion. PMID:24579044

  6. 富血小板血纤蛋白及其应用进展%Platelet-rich fibrin and its applications progression

    Institute of Scientific and Technical Information of China (English)

    王萍; 向琳

    2011-01-01

    Platelet-rich fibrin (PRF) is a new generation of platelet concentrate developed by Choukroun in 2000. Its processing is simple and without any additives, reducing the risk of infectious diseases' transmission. PRF fibrin network presents biochemical architecture of connected trimolecular or equilateral junctions. Thus, the flexible and elastic fibrin network is able to support cytokines enmeshment and cellular migration. It is rich in platelets and cytokines that can promote tissue healing and regeneration. It can also regulate inflammatory reactions. We present a review on PRF's biological characteristics, regulation mechanism and clinical applications.%富血小板血纤蛋白(PRF)是由法国科学家Choukroun于2000年提出的新一代血小板浓缩物.PRF制备简便,无任何人工或生物制剂,降低了感染性疾病传播的风险;其血纤蛋白的立体网状三分子结构柔韧而有弹性,可将细胞因子等包埋嵌合于其孔隙中缓慢释放,为迁徙细胞提供支架;富含的诸多与组织愈合和骨修复相关的细胞因子,可促进组织的愈合;其免疫细胞释放的炎症因子,具有调节炎症反应和抗感染的作用.本文就PRF的生物学特性、PRF促进组织修复的机制、PRF的临床应用等研究进展作一综述.

  7. CONTAMINATED PROBLEMATIC SKIN WOUNDS IN DIABETIC PATIENTS TREATED WITH AUTOLOGOUS PLATELET-RICH PLASMA (PRP: A case series study

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    Tsvetan Sokolov

    2016-03-01

    Full Text Available OBJECTIVE: To study the effect of platelet-rich plasma (PRP on contaminated problematic skin ulcers in patients with diabetes. MATERIAL AND METHODS: A total of 6 patients had been treated within the period from 2012 to 2014; they had various types of problematic wounds and diabetes type 2. Patients’ distribution by sex was as follows: 1 man and 5 women; mean age- 68 years. Ulcer types: acute (2 patients, hard-to-heal (2 patients and chronic (2 patients ulcers. The mean size of the skin and soft tissue defect was 9,5 cm2. Pathogenic microflora was isolated in 4 patients - S. aureus in three and Е. Coli in one. Based on a scheme developed by us, all cases were treated by administering platelet-rich plasma, derived by PRGF Endoret system. Follow-up period was within 4 – 6 months (4,5 on average. We used platelet rich plasma derived by PRGF Endoret system, applied on the wound bed on a weekly basis. RESULTS: Application of PRP allowed successful closure of all wounds. There were no complications associated with treatment of PRP. Epithelialization of the wound took 15 weeks on average for all patients. One patient presented with hyperkeratosis. Initial score of followed wounds, based on the scales are as follows: Total wound score – 10 p. Total anatomic score – 8 p. Total score – 15 p. at the initial stage. At the end of the treatment period scores were as follows - 0 p., which means excellent results CONCLUSION: We believe that the application of PRP may become optimal therapy in the treatment of contaminated problematic wounds in diabetic patients. PRP not only stimulates wound healing, but also has antimicrobial properties, which may contribute to the prevention of infections.

  8. The Chondrogenic Induction Potential for Bone Marrow-Derived Stem Cells between Autologous Platelet-Rich Plasma and Common Chondrogenic Induction Agents: A Preliminary Comparative Study

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    Shan-zheng Wang

    2015-01-01

    Full Text Available The interests in platelet-rich plasma (PRP and their application in stem cell therapy have contributed to a better understanding of the basic biology of the prochondrogenesis effect on bone marrow-derived stem cells (BMSCs. We aimed at comparing the effect of autologous PRP with common chondrogenic induction agents (CCIAs on the chondrogenic differentiation of BMSCs. Rabbit BMSCs were isolated and characterized by flow cytometry and differentiated towards adipocytes and osteoblasts. The chondrogenic response of BMSCs to autologous PRP and CCIAs which included transforming growth factor-β1 (TGF-β1, dexamethasone (DEX, and vitamin C (Vc was examined by cell pellet culture. The isolated BMSCs after two passages highly expressed CD29 and CD44 but minimally expressed CD45. The osteogenic and adipogenic differentiation potentials of the isolated BMSCs were also confirmed. Compared with common CCIAs, autologous PRP significantly upregulated the chondrogenic related gene expression, including Col-2, AGC, and Sox-9. Osteogenic related gene expression, including Col-1 and OCN, was not of statistical significance between these two groups. Thus, our data shows that, compared with common chondrogenic induction agents, autologous PRP can be more effective in promoting the chondrogenesis of BMSCs.

  9. Plasma components and platelet activation are essential for the antimicrobial properties of autologous platelet-rich plasma: an in vitro study.

    Directory of Open Access Journals (Sweden)

    Lorenzo Drago

    Full Text Available Autologous platelet concentrates are successfully adopted in a variety of medical fields to stimulate bone and soft tissue regeneration. The rationale for their use consists in the delivery of a wide range of platelet-derived bioactive molecules that promotes wound healing. In addition, antimicrobial properties of platelet concentrates have been pointed out. In this study, the effect of the platelet concentration, of the activation step and of the presence of plasmatic components on the antimicrobial activity of pure platelet-rich plasma was investigated against gram positive bacteria isolated from oral cavity. The antibacterial activity, evaluated as the minimum inhibitory concentration, was determined through the microdilution two-fold serial method. Results seem to suggest that the antimicrobial activity of platelet-rich plasma against Enterococcus faecalis, Streptococcus agalactiae, Streptococcus oralis and Staphylococcus aureus is sustained by a co-operation between plasma components and platelet-derived factors and that the activation of coagulation is a fundamental step. The findings of this study may have practical implications in the modality of application of platelet concentrates.

  10. A comparative study of the regenerative effect of sinus bone grafting with platelet-rich fibrin-mixed Bio-Oss® and commercial fibrin-mixed Bio-Oss®: an experimental study.

    Science.gov (United States)

    Xuan, Feng; Lee, Chun-Ui; Son, Jeong-Seog; Jeong, Seung-Mi; Choi, Byung-Ho

    2014-06-01

    Anorganic bovine bone (Bio-Oss®) particles are one of the most popular grafting materials. The particles are often mixed with platelet-rich fibrin (PRF) or a commercial fibrin (Tisseel®) to form a mouldable graft material. The objective of this study was to compare the potentials of PRF-mixed Bio-Oss® and Tisseel®-mixed Bio-Oss® to enhance bone regeneration in a canine sinus model. Six mongrel dogs were used in this study. After elevating the sinus membrane in both maxillary sinus cavities, an implant was placed into the sinus cavity. In one of the sinus cavities, the PRF/Bio-Oss® composite was grafted, and the Tisseel®/Bio-Oss® composite was grafted in the other sinus cavity. After a 6 month healing period, bone formation in the graft sites and bone-implant contact were evaluated. The mean osseointegration rate was 43.5 ± 12.4% and new bone formation rate 41.8 ± 5.9% in the PRF/Bio-Oss® composite sites. In the Tisseel®/Bio-Oss® composite sites they were 30.7 ± 7.9% and 31.3 ± 6.4%. There were statistically significant differences between the groups. The findings from this study suggest that when platelet-rich fibrin is used as an adjunct to Bio-Oss® particles for bone augmentation in the maxillary sinus, bone formation in the graft sites is significantly greater than when Tisseel® is used. PMID:23911120

  11. Autologous bone grafting with platelet-rich plasma for alveolar cleft repair in patient with cleft and palate.

    Science.gov (United States)

    Giudice, Giuseppe; Cutrignelli, Daniela Anna; Leuzzi, Sara; Robusto, Fabio; Sportelli, Pasquale; Nacchiero, Eleonora

    2016-01-01

    L’innesto osseo è essenziale nella terapia chirurgica della labiopalatoschisi (LPS), ma i tempi di osteogenesi ed integrazione ossea nella sede ricevente possono procrastinare i successivi interventi di ortodonzia. Nel nostro studio è stata valutata la capacità della PRP (platelet-rich plasma) associata al trapianto osseo nel diminuire il tempo d’attesa e la durata del trattamento ortodontico in una coorte di 8 bambini, confrontandola con 8 controlli sottoposti a semplice innesto osseo. I risultati hanno mostrato come i soggetti sottoposti a trapianto osseo + PRP abbiano avuto la possibilità di essere sottoposti ad un trattamento ortodontico più precoce (155 giorni vs 298) e più breve (295 giorni vs 356) rispetto al gruppo di controllo. Inoltre nel gruppo di controllo si sono verificate con maggiore frequenza complicanze post-chirurgiche (quali fistole oro-nasali, disturbi periodontali, malattie dentali, perdita di spessore, massa o trabecolatura ossea) rispetto al gruppo sottoposto a PRP. Perciò l’associazione della PRP (platelet-rich plasma) al trapianto osseo è una metodica che sembra associarsi ad una diminuzione statisticamente significativa dei tempi di integrazione ossea nei pazienti affetti da LPS, con conseguente anticipazione della ortodonzia e miglioramento degli outcome chirurgici.

  12. Do the fibrin architecture and leukocyte content influence the growth factor release of platelet concentrates? An evidence-based answer comparing a pure platelet-rich plasma (P-PRP) gel and a leukocyte- and platelet-rich fibrin (L-PRF).

    Science.gov (United States)

    Dohan Ehrenfest, David M; Bielecki, Tomasz; Jimbo, Ryo; Barbé, Giovanni; Del Corso, Marco; Inchingolo, Francesco; Sammartino, Gilberto

    2012-06-01

    Platelet concentrates for surgical use are tools of regenerative medicine designed for the local release of platelet growth factors into a surgical or wounded site, in order to stimulate tissue healing or regeneration. Leukocyte content and fibrin architecture are 2 key characteristics of all platelet concentrates and allow to classify these technologies in 4 families, but very little is known about the impact of these 2 parameters on the intrinsic biology of these products. In this demonstration, we highlight some outstanding differences in the growth factor and matrix protein release between 2 families of platelet concentrate: Pure Platelet-Rich Plasma (P-PRP, here the Anitua's PRGF - Preparation Rich in Growth Factors - technique) and Leukocyte- and Platelet-Rich Fibrin (L-PRF, here the Choukroun's method). These 2 families are the extreme opposites in terms of fibrin architecture and leukocyte content. The slow release of 3 key growth factors (Transforming Growth Factor β1 (TGFβ1), Platelet-Derived Growth Factor AB (PDGF-AB) and Vascular Endothelial Growth Factor (VEGF)) and matrix proteins (fibronectin, vitronectin and thrombospondin-1) from the L-PRF and P-PRP gel membranes in culture medium is described and discussed. During 7 days, the L-PRF membranes slowly release significantly larger amounts of all these molecules than the P-PRP gel membranes, and the 2 products display different release patterns. In both platelet concentrates, vitronectin is the sole molecule to be released almost completely after only 4 hours, suggesting that this molecule is not trapped in the fibrin matrix and not produced by the leukocytes. Moreover the P-PRP gel membranes completely dissolve in the culture medium after less than 5 days only, while the L-PRF membranes are still intact after 7 days. This simple demonstration shows that the polymerization and final architecture of the fibrin matrix considerably influence the strength and the growth factor trapping/release potential

  13. Influence of Leukocyte- and Platelet-Rich Fibrin (L-PRF) in the Healing of Simple Postextraction Sockets: A Split-Mouth Study.

    Science.gov (United States)

    Marenzi, Gaetano; Riccitiello, Francesco; Tia, Mariano; di Lauro, Alessandro; Sammartino, Gilberto

    2015-01-01

    The aim of this study was to evaluate the effects of leukocyte- and platelet-rich fibrin (L-PRF) on the pain and soft tissue healing after tooth extractions. Twenty-six patients (9 males and 17 females) were treated with multiple extractions (2 to 8), with a total of 108 extractions. This was an exploratory single blinded randomized clinical trial with a split-mouth design. The pain after the surgery was assessed in each patient by the VAS scale (1 to 10) at intervals of 24-48-72-96 hours. The soft tissue healing was clinically evaluated at 3, 7, 14, and 21 days after surgery by the same examiner surgeon, using the modified Healing Index (4 to 12). The mean value of postextraction pain was 3.2 ± 0.3 in the experimental sides and 4.1 ± 0.1 in the control sides. After 7 days from the extractions, the values of modified Healing Index in the experimental and control groups were, respectively, 4.8 ± 0.6 and 5.1 ± 0.9. The use of L-PRF in postextraction sockets filling can be proposed as a useful procedure in order to manage the postoperative pain and to promote the soft tissue healing process, reducing the early adverse effects of the inflammation.

  14. A comparative evaluation of extraction socket preservation with demineralized freeze-dried bone allograft alone and along with platelet-rich fibrin: A clinical and radiographic study

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    Dhaval J Thakkar

    2016-01-01

    Full Text Available Aims: To investigate clinically and radiographically, the bone fill in extraction sockets using demineralized freeze-dried bone allograft alone and along with platelet-rich fibrin (PRF. Materials and Methods: A randomized controlled clinical trial was carried out on 36 nonrestorable single-rooted teeth sites. Sites were randomized into demineralized freeze-dried bone allograft (DFDBA combined with PRF - test and DFDBA - control groups using a coin toss method. After the placement of graft material, collagen membrane was used to cover it. The clinical parameters recorded were ridge width and ridge height. All the parameters were recorded at baseline and at 90 and 180 days. Statistical Analysis Used: Independent t-test and paired t-test. Results: In both groups, there is significant reduction in loss of ridge width and ridge height from baseline to 90 days (P < 0.001, baseline to 180 days (P < 0.001, and 90-180 days (P < 0.001. However, when both the groups were compared the test group favored in the reduction of ridge width while there was no statistical difference in reduction of ridge height among at different intervals. Conclusions: Although DFDBA is considered as an ideal graft material, PRF can be used as an adjunctive with DFDBA for socket preservation.

  15. 富血小板纤维蛋白的研究进展%Research progress on platelet-rich fibrin

    Institute of Scientific and Technical Information of China (English)

    杨世茂

    2012-01-01

    Platelet-rich fibrin (PRF) is a source of autogenic neotype biomaterial, which is rich in cytokine and growth factors, and is called new generation of platelet concentrates. The molecular constitution of PRF is similar to nature blood clot, and to provide location with migration, proliferation, and differentiation for rhagiocrine cell. Recently, many researchers use PRF as graft on the sinus floor elevation in dental implantation and win well results. This article reviewed PRF and its application on sinus floor elevation in dental implantation.%富血小板纤维蛋白(PRF)是一种富含细胞因子和生长因子的自体来源的新型生物材料,被誉为新一代血小板浓缩物.其分子结构类似天然血凝块,为组织细胞提供迁移、增殖和分化的场所.近来,许多学者将PRF作为移植材料应用于口腔种植前上颌窦底提升术后的骨移植中,并获得了良好的效果.本文就PRF 及其在口腔种植上颌窦底提升术中的应用进展作一综述.

  16. Ginsenoside Rg1 and platelet-rich fibrin enhance human breast adipose-derived stem cell function for soft tissue regeneration.

    Science.gov (United States)

    Xu, Fang-Tian; Liang, Zhi-Jie; Li, Hong-Mian; Peng, Qi-Liu; Huang, Min-Hong; Li, De Quan; Liang, Yi-Dan; Chi, Gang-Yi; Li, De Hui; Yu, Bing-Chao; Huang, Ji-Rong

    2016-06-01

    Adipose-derived stem cells (ASCs) can be used to repair soft tissue defects, wounds, burns, and scars and to regenerate various damaged tissues. The cell differentiation capacity of ASCs is crucial for engineered adipose tissue regeneration in reconstructive and plastic surgery. We previously reported that ginsenoside Rg1 (G-Rg1 or Rg1) promotes proliferation and differentiation of ASCs in vitro and in vivio. Here we show that both G-Rg1 and platelet-rich fibrin (PRF) improve the proliferation, differentiation, and soft tissue regeneration capacity of human breast adipose-derived stem cells (HBASCs) on collagen type I sponge scaffolds in vitro and in vivo. Three months after transplantation, tissue wet weight, adipocyte number, intracellular lipid, microvessel density, and gene and protein expression of VEGF, HIF-1α, and PPARγ were higher in both G-Rg1- and PRF-treated HBASCs than in control grafts. More extensive new adipose tissue formation was evident after treatment with G-Rg1 or PRF. In summary, G-Rg1 and/or PRF co-administration improves the function of HBASCs for soft tissue regeneration engineering.

  17. The effect of a platelet-rich fibrin conduit on neurosensory recovery following inferior alveolar nerve lateralization: a preliminary clinical study.

    Science.gov (United States)

    Khojasteh, A; Hosseinpour, S; Nazeman, P; Dehghan, M M

    2016-10-01

    This retrospective study aimed to assess the recovery of neurosensory dysfunction following modified inferior alveolar nerve (IAN) lateralization surgery compared to the conventional approach. Data from two groups of patients who underwent IAN lateralization in 2014 were included in this study. In one group, platelet-rich fibrin was placed over the IAN and this was protected with a collagen membrane conduit; the other group underwent the conventional IAN lateralization procedure. Implants were placed immediately. Neurosensory dysfunction was evaluated at 3, 6, and 12 months post-surgery. Demographic, neurosensory disturbance (NSD), subjective two-point discrimination test (TPD), and static light touch test (SLT) data were obtained. Twenty-three IAN lateralization procedures with the placement of 51 implants were performed in 14 patients. At the 6-month follow-up, the number of patients experiencing normal sensation was greater in the modified surgery group, but the 12-month follow-up results were the same in the two groups. More precise sensation was observed with the TPD in the modified group at 6 months, and the modified group demonstrated better SLT scores at 6 months. Although the two groups had comparable results at the 12-month follow-up, it was observed that the modified technique accelerated neural healing within 6 months and reduced the length of the discomfort period.

  18. Efficacy of platelet rich fibrin in the treatment of human intrabony defects with or without bone graft: A randomized controlled trial

    Science.gov (United States)

    Chandradas, Nikhil D.; Ravindra, Shivamurthy; Rangaraju, Vivekananda M.; Jain, Sheetal; Dasappa, Shivaprasad

    2016-01-01

    Aim: To evaluate the efficacy of platelet rich fibrin (PRF) with or without bone graft [demineralized bone matrix (DBM) graft] in the treatment of intrabony defects based on clinical and radiographic parameters. Materials and Methods: Thirty six intrabony defects in 36 patients were randomly divided into three different groups and were treated with group A (PRF with DBM) or group B (PRF alone) or group C [open flap debridement (OFD)]. Clinical parameters such as plaque index (PI), gingival index (GI), probing depth (PD), relative attachment level (RAL), and gingival recession (GR) were assessed at baseline and 9 months postoperatively; radiographic parameters such as linear bone growth (LBG) and percentage in bone fill (%BF) were calculated by using the image analysis software. Comparisons of groups were analyzed using Kruskal–Wallis analysis of variance test. Pair-wise comparison of groups was done by Mann-Whitney U test. Results: Mean PD reduction and RAL gain were greater in group A (4.25 ± 1.48, 3.92 ± 0.90) and group B (3.82 ± 0.75, 3.27 ± 0.65) than control (3.00 ± 1.21, 2.25 ± 0.62). Furthermore, statistically significant improvement in LBG and %BF was found in group A (3.47 ± 0.53, 61.53 ± 4.54) compared to group B (2.55 ± 0.61, 49.60 ± 14.08) and group C (1.21 ± 0.80, 24.69 ± 15.59). Conclusions: The study demonstrated that PRF improves clinical and radiological parameters compared to OFD alone in intrabony defects. Addition of DBM enhances the effects of PRF in RAL gain and radiographic defect fill. PMID:27652249

  19. Platelet-rich plasma and fibrin glue-coated bioactive ceramics enhance growth and differentiation of goat bone marrow-derived stem cells.

    Science.gov (United States)

    Nair, Manitha B; Varma, H K; John, Annie

    2009-07-01

    New biotechnologies such as tissue engineering require functionally active cells within supportive matrices where the physical and chemical stimulus provided by the matrix is indispensable to determine the cellular behavior. This study has investigated the influence of platelet-rich plasma (PRP) and fibrin glue (FG) on the functional activity of goat bone marrow-derived mesenchymal stem cells (gBMSCs) that differentiated into the osteogenic lineage. To achieve this goal, PRP and FG were separately coated on bioactive ceramics like hydroxyapatite (HA) and silica-coated HA (HASi), on which gBMSCs were seeded and induced to differentiate into the osteogenic lineage for 28 days. The cells were then analyzed for viability (lactate dehydrogenase assay: acridine orange and ethidium bromide staining), morphology (scanning electron microscopy), proliferation (picogreen assay), cell cycle assay (propidium iodide staining), and differentiation (alkaline phosphatase [ALP] activity and real-time PCR analysis of ALP, osteocalcin, and osteopontin gene). It has been observed that PRP and FG have appreciably favored the viability, spreading, and proliferation of osteogenic-induced gBMSCs. The osteopontin and osteocalcin expression was significantly enhanced on PRP- and FG-coated HA and HASi, but PRP had effect on neither ALP expression nor ALP activity. The results of this study have depicted that FG-coated ceramics were better than PRP-coated and bare matrices. Among all, the excellent performance was shown by FG coated HASi, which may be attributed to the communal action of the stimulus emanated by Si in HASi and the temporary extracellular matrix provided by FG over HASi. Thus, we can conclude that PRP or FG in combination with bioactive ceramics could possibly enhance the functional activity of cells to a greater extent, promoting the hybrid composite as a promising candidate for bone tissue engineering applications.

  20. Leukocyte- and platelet-rich fibrin (L-PRF) for long-term delivery of growth factor in rotator cuff repair: review, preliminary results and future directions.

    Science.gov (United States)

    Zumstein, Matthias A; Berger, Simon; Schober, Martin; Boileau, Pascal; Nyffeler, Richard W; Horn, Michael; Dahinden, Clemens A

    2012-06-01

    Surgical repair of the rotator cuff repair is one of the most common procedures in orthopedic surgery. Despite it being the focus of much research, the physiological tendon-bone insertion is not recreated following repair and there is an anatomic non-healing rate of up to 94%. During the healing phase, several growth factors are upregulated that induce cellular proliferation and matrix deposition. Subsequently, this provisional matrix is replaced by the definitive matrix. Leukocyte- and platelet-rich fibrin (L-PRF) contain growth factors and has a stable dense fibrin matrix. Therefore, use of LPRF in rotator cuff repair is theoretically attractive. The aim of the present study was to determine 1) the optimal protocol to achieve the highest leukocyte content; 2) whether L-PRF releases growth factors in a sustained manner over 28 days; 3) whether standard/gelatinous or dry/compressed matrix preparation methods result in higher growth factor concentrations. 1) The standard L-PRF centrifugation protocol with 400 x g showed the highest concentration of platelets and leukocytes. 2) The L-PRF clots cultured in medium showed a continuous slow release with an increase in the absolute release of growth factors TGF-β1, VEGF and MPO in the first 7 days, and for IGF1, PDGF-AB and platelet activity (PF4=CXCL4) in the first 8 hours, followed by a decrease to close to zero at 28 days. Significantly higher levels of growth factor were expressed relative to the control values of normal blood at each culture time point. 3) Except for MPO and the TGFβ-1, there was always a tendency towards higher release of growth factors (i.e., CXCL4, IGF-1, PDGF-AB, and VEGF) in the standard/gelatinous- compared to the dry/compressed group. L-PRF in its optimal standard/gelatinous-type matrix can store and deliver locally specific healing growth factors for up to 28 days and may be a useful adjunct in rotator cuff repair.

  1. Alignment of the Fibrin Network Within an Autologous Plasma Clot.

    Science.gov (United States)

    Gessmann, Jan; Seybold, Dominik; Peter, Elvira; Schildhauer, Thomas Armin; Köller, Manfred

    2016-01-01

    Autologous plasma clots with longitudinally aligned fibrin fibers could serve as a scaffold for longitudinal axonal regrowth in cases of traumatic peripheral nerve injuries. Three different techniques for assembling longitudinally oriented fibrin fibers during the fibrin polymerization process were investigated as follows: fiber alignment was induced by the application of either a magnetic field or-as a novel approach-electric field or by the induction of orientated flow. Fiber alignment was characterized by scanning electron microscopy analysis followed by image processing using fast Fourier transformation (FFT). Besides FFT output images, area xmin to xmax, as well as full width at half maximum (FWHM) of the FFT graph plot peaks, was calculated to determine the relative degree of fiber alignment. In addition, fluorescently labeled human fibrinogen and mesenchymal stem cells (MSCs) were used to visualize fibrin and cell orientation in aligned and nonaligned plasma clots. Varying degrees of fiber alignment were achieved by the three different methods, with the electric field application producing the highest degree of fiber alignment. The embedded MSCs showed a longitudinal orientation in the electric field-aligned plasma clots. The key feature of this study is the ability to produce autologous plasma clots with aligned fibrin fibers using physical techniques. This orientated internal structure of an autologous biomaterial is promising for distinct therapeutic applications, such as a guiding structure for cell migration and growth dynamics.

  2. Efficacy of autologous platelet-rich plasma combined with fractional ablative carbon dioxide resurfacing laser in treatment of facial atrophic acne scars: A split-face randomized clinical trial

    Directory of Open Access Journals (Sweden)

    Gita Faghihi

    2016-01-01

    Full Text Available Background: Autologous platelet-rich plasma has recently attracted significant attention throughout the medical field for its wound-healing ability. Aims: This study was conducted to investigate the potential of platelet-rich plasma combined with fractional laser therapy in the treatment of acne scarring. Methods: Sixteen patients (12 women and 4 men who underwent split-face therapy were analyzed in this study. They received ablative fractional carbon dioxide laser combined with intradermal platelet-rich plasma treatment on one half of their face and ablative fractional carbon dioxide laser with intradermal normal saline on the other half. The injections were administered immediately after laser therapy. The treatment sessions were repeated after an interval of one month. The clinical response was assessed based on patient satisfaction and the objective evaluation of serial photographs by two blinded dermatologists at baseline, 1 month after the first treatment session and 4 months after the second. The adverse effects including erythema and edema were scored by participants on days 0, 2, 4, 6, 8, 15 and 30 after each session. Results: Overall clinical improvement of acne scars was higher on the platelet-rich plasma-fractional carbon dioxide laser treated side but the difference was not statistically significant either 1 month after the first treatment session (P = 0.15 or 4 months after the second (P = 0.23. In addition, adverse effects (erythema and edema on the platelet-rich plasma-fractional carbon dioxide laser-treated side were more severe and of longer duration. Limitations: Small sample size, absence of all skin phototypes within the study group and lack of objective methods for the evaluation of response to treatment and adverse effects were the limitations. Conclusion: This study demonstrated that adding platelet-rich plasma to fractional carbon dioxide laser treatment did not produce any statistically significant synergistic effects

  3. Management of localized advance loss of periodontal support associated Grade II furcation and intrabony defect in chronic periodontitis patient through amalgamation of platelet-rich fibrin and hydroxyapatite bioactive glass composite granules.

    Science.gov (United States)

    Salaria, Sanjeev Kumar; Ghuman, Simrat Kaur; Kumar, Saurabh; Sharma, Garima

    2016-01-01

    Periodontal disease is infectious, complex, multifactorial, chronic inflammatory disease of supporting periodontal tissues that not only alters the bone morphology but also leads to the reduction in bone height. Different types of bony deformities such as horizontal, vertical, craters, and furcation result from periodontal disease, but vertical and Grade II furcation defects are more amenable to regenerative periodontal therapy. The present case report describes the current concept of periodontal diagnosis and the clinical radiographical efficiency of platelet-rich fibrin and hydroxyapatite bioactive glass composite granules graft combination in the management of localized advance osseous defects with respect to tooth number 36 in chronic periodontitis patient at 1 year postoperatively. PMID:27630511

  4. Management of localized advance loss of periodontal support associated Grade II furcation and intrabony defect in chronic periodontitis patient through amalgamation of platelet-rich fibrin and hydroxyapatite bioactive glass composite granules

    Science.gov (United States)

    Salaria, Sanjeev Kumar; Ghuman, Simrat Kaur; Kumar, Saurabh; Sharma, Garima

    2016-01-01

    Periodontal disease is infectious, complex, multifactorial, chronic inflammatory disease of supporting periodontal tissues that not only alters the bone morphology but also leads to the reduction in bone height. Different types of bony deformities such as horizontal, vertical, craters, and furcation result from periodontal disease, but vertical and Grade II furcation defects are more amenable to regenerative periodontal therapy. The present case report describes the current concept of periodontal diagnosis and the clinical radiographical efficiency of platelet-rich fibrin and hydroxyapatite bioactive glass composite granules graft combination in the management of localized advance osseous defects with respect to tooth number 36 in chronic periodontitis patient at 1 year postoperatively. PMID:27630511

  5. Pulp revascularization using platelet rich plasma autologous or in conjunction with a collagen matrix as a therapeutic possibility for teeth with an open apex necrotic pulp and / or periapical pathology.

    OpenAIRE

    Camargo Guevara, Paula Alejandra; Sossa Rojas, Henry

    2014-01-01

    Objective: Describe the use of the Autologous Platelet-Rich Plasma or in combination with a collagen matrix as a potential revascularization therapy in teeth with open apices, necrotic pulp and/or periapical lesion. Methods: A thematic literature searchwas conducted from 2007 to 2013 using electronic means,  databases and journals with high impact on endodontic. Results: Regenerative endodontic is a biological based procedure  designed to replace structures that have been damaged, diseased or...

  6. The application of platelet-rich plasma(PRP) and platelet-rich fibrin(PRF) in periodontal tissue regeneration%富血小板血浆与富血小板纤维蛋白在牙周组织再生中的应用

    Institute of Scientific and Technical Information of China (English)

    钱文慧; 徐艳; 孙颖

    2011-01-01

    富血小板血浆(platelet-rich plasma,PRP)与富血小板纤维蛋白(platelet-rich fibrin,PRF)因其促进组织再生的能力,在临床口腔创伤及缺损的修复中逐渐被重视及应用,并取得了令人较为满意的效果,鉴于PRP与PRF的制作较简便,且不易出现疾病传染及免疫排斥的不良反应,两者在口腔临床中的应用越来越广泛.牙周炎是目前造成牙周骨缺损最为常见的疾病之一,国内外的学者们为了探寻促进牙周组织再生的方法,将PRP与PRF用于牙周治疗中,本文即将近年来有关PRP与PRF在牙周组织再生中的临床应用作一综述.

  7. Autologous platelet rich plasma repair facial nerve injury%自体富血小板血浆修复面神经损伤★

    Institute of Scientific and Technical Information of China (English)

    张兴安; 吴蜀江; 卢海彬; 石修全; 王洪玲; 曹云亮; 李元秀

    2013-01-01

      背景:周围性面神经损伤治疗包括手术、理疗及药物等方法,但有些情况下治疗效果并不十分理想。目的:研究自体富血小板血浆在面神经损伤修复中的作用。方法:将健康大耳白兔10只双侧面神经上颊支横断后置入硅胶神经再生导管,一侧注入富血小板血浆为实验侧,另一侧注入生理盐水为对照侧。术后8周进行面神经大体观察、神经电生理检测、组织学观察、图像分析、评价面神经再生恢复情况。结果与结论:实验侧口轮匝肌动作电位潜伏期明显低于对照侧,复合神经肌肉动作电位振幅(M 波)明显高于对照侧(P <0.01)。实验侧再生神经更显成熟,再生轴突较多,髓鞘分化较好,髓鞘厚度较均匀,再生轴突的直径接近正常,神经轴突较密集,排列较规则,神经纤维外膜较较对照组增厚,胶原纤维、弹力纤维层较对照组增多;对照侧再生轴突数目较少,分布不均匀,轴突发育较差,并见大量纤维结缔组织,空泡变性较实验侧为多。实验侧再生神经在有髓轴突直径、面积、髓鞘厚度及轴突计数等方面均明显优于对照侧,两组差异有显著性(P <0.01)。提示富血小板血浆在面神经损伤修复再生中具有促进作用。%BACKGROUND: Therapeutic methods for of peripheral facial nerve injury include surgery, physical therapy and drug treatment, but the treatment effect is not ideal in some certain cases. OBJECTIVE: To study the effect of autologous platelet rich plasma on repair of facial nerve injury. METHODS: The bilateral destroyed buccal nerve branches of the 10 white rabbits were put in silica gel nerve regeneration chamber, one side injected with platelet rich plasma as experimental group, the other side injected with normal saline as control group. The general observation, neuroelectrophysiology detection, histological observation, image analysis and

  8. Long palatal connective tissue rolled pedicle graft with demineralized freeze-dried bone allograft plus platelet-rich fibrin combination: A novel technique for ridge augmentation - Three case reports

    Directory of Open Access Journals (Sweden)

    Pathakota Krishnajaneya Reddy

    2015-01-01

    Full Text Available Replacement of missing maxillary anterior tooth with localized residual alveolar ridge defect is challenging, considering the high esthetic demand. Various soft and hard tissue procedures were proposed to correct alveolar ridge deformities. Novel techniques have evolved in treating these ridge defects to improve function and esthetics. In the present case reports, a novel technique using long palatal connective tissue rolled pedicle graft with demineralized freeze-dried bone allografts (DFDBAs plus Platelet-rich fibrin (PRF combination was proposed to correct the Class III localized anterior maxillary anterior alveolar ridge defect. The present technique resulted in predictable ridge augmentation, which can be attributed to the soft and hard tissue augmentation with a connective tissue pedicle and DFDBA plus PRF combination. This technique suggests a variation in roll technique with DFDBA plus PRF and appears to promise in gaining predictable volume in the residual ridge defect and can be considered for the treatment of moderate to severe maxillary anterior ridge defects.

  9. Effect of autologous platelet-rich plasma on the wound healing of cosmetic surgery%自体富血小板血浆促进美容外科伤口愈合的临床观察

    Institute of Scientific and Technical Information of China (English)

    程飚; 刘宏伟; 唐建兵; 肖丽玲; 张宏斌; 单桂秋

    2011-01-01

    目的 观察与评价自体富血小板血浆在美容整形外科中的应用效果.方法 选取2006年2月~2010年12月共6名在广州军区广州总医院激光整形美容中心和暨南大学第一附属医院整形烧伤外科接受美容整形手术治疗的患者,其中面部除皱2例,双颞部凹陷自体脂肪充填2例,自体脂肪隆胸1例,乳房提升术1例.术前抽取患者静脉血液20~60 ml.通过离心技术收集富血小板成分的血浆,在一侧术区外用,对侧不做其他特殊处理.结果 随访时间1~2年,使用自体富血小板血浆的一侧,创面愈合质量有显著提升,瘢痕减少.结论 自体富血小板血浆能显著提高美容整形手术的愈合能力及自体脂肪移植的成活率,且该方法 操作简单,可行性强,值得临床推广.%Objective To observe and evaluate the efficacy of autologous platelet-rich plasma on wound healing after cosmetic operation. Methods Six female patients undergoing plastic surgery were recruited from Guangzhou General Hospital of Guangzhou Military Region and the First Affiliated Hospital of Jinan University,from Feb,2006 to Dec,2010. Two cases underwent face lifting,2 autologous fat filling of double-temporal depression, 1 breast lifting with autologous fat, 1 reduction mammaplasty. Twenty to sixty milliliters of venous blood was withdrawn from each patient prior to surgery and platelet rich plasma was prepared. The wounds on one side of the body were treated with autologous platelet-rich plasma on top of standard wound care, and those on the other side were treated with standard care to serve as controls. The quality of wound healing was assessed. Results After 1 to 2 years of follow-up, the healing of the wounds treated by autologous platelet-rich plasma was significantly better with reduced scars,compared to the control wounds. The survival of the injected fat was better as compared to the control. Conclusion Autologous platelet-rich plasma can significantly

  10. Platelet-rich plasma (PRP for acute muscle injury: a systematic review.

    Directory of Open Access Journals (Sweden)

    Mohamad Shariff A Hamid

    Full Text Available INTRODUCTION: Acute muscle injury is one of the commonest injuries that often result in loss of training and competition time. The best management for muscle injury has not been identified. Sports medicine practitioners used several approaches in attempt to accelerate time to recovery from muscle injury. More recently growing interest focussed on autologous blood product injection. METHODS: A literature search was conducted systematically using OvidMEDLINE, PubMed, EMBASE, SPORTDiscus and CINAHL databases to retrieve articles published until December 2012. Controlled trials and controlled laboratory studies comparing different strategies to promote early recovery of muscle injury were included. The methodological quality of studies was assessed. RESULTS: There are limited studies on the effects of PRP therapy for muscle injury. Three in vivo laboratory studies and one pilot human study were reviewed. The laboratory studies reported histological evidence on significant acceleration of muscle healing in animals treated with autologous conditioned serum (ACS, platelet-rich plasma (PRP and platelet rich fibrin matrix (PRFM. A pilot human study found athletes treated with repeated ACS injection recovers significantly faster than retrospective controls. CONCLUSION: Several in vivo laboratory studies suggest beneficial effects of ACS, PRP and PRFM in accelerating muscle recovery. Evidence to suggest similar effects on humans is however limited, as valuable information from robust human controlled trials is still not available at this moment. Hence, more studies of satisfactory methodological quality with platelet-rich plasma interventions on muscle injury are justified.

  11. Study on the effect of platelet rich fibrin on the growth, production of acid and adhesion of Streptococcus%富血小板纤维蛋白对变形链球菌影响的研究

    Institute of Scientific and Technical Information of China (English)

    齐鹏鹏; 王景云; 孟粼; 于士洋; 李雨珊; 王红红

    2016-01-01

    目的 研究富血小板纤维蛋白(platelet-rich ibrin,PRF)对变形链球菌生长、产酸和粘附的影响.方法 选用变形链球菌标准菌株UA 159,制备PRF膜片及PRF浸出液(Platelet-rich fibrin extract,PRFe),将变形链球菌标准菌株UA159菌悬液接种于含有不同数量PRF膜片的BHI液体培养基中,培养48小时后,观察PRF对变形链球茵生长、产酸影响;用含有不同浓度PRF浸出液的BHI培养液培养变形链球菌48小时后,观察PRF对变形链球菌粘附性的影响.结果 PRF对UA159生长、产酸和粘附均有抑制作用,且随着PRF膜片数量的增加或PRF浸出液浓度的增高抑制作用逐渐增强.结论 PRF能抑制变形链球茵生长、产酸和粘附,并且随着PRF膜片量数的增加或PRF浸出液浓度的增高,抑制作用增强.

  12. Transplantation of autologous keratinocyte suspension in fibrin matrix to chronic venous leg ulcers: improved long-term healing after removal of the fibrin carrier.

    NARCIS (Netherlands)

    Hartmann, A.; Quist, J.; Hamm, H.; Brocker, E.B.; Friedl, P.H.A.

    2008-01-01

    BACKGROUND: The transplantation of keratinocytes suspended in fibrin carrier represents a candidate regimen for chronic ulcer treatment in an outpatient setting. We evaluated the integration and survival of autologous individualized keratinocytes applied within fibrin matrix onto chronic venous leg

  13. Comparative study between fibrin glue and platelet rich plasma in dogs skin grafts Estudo comparativo entre a cola de fibrina e o plasma rico em plaquetas em enxertos cutâneos em cães

    Directory of Open Access Journals (Sweden)

    Larissa Correa Hermeto

    2012-11-01

    Full Text Available PURPOSE: Compare fibrin glue (Tissucol® and platelet-rich plasma in full-thickness mesh skin grafts in dogs. METHODS: Eighteen dogs were used, divided into two groups: fibrin glue (FG and platelet-rich plasma (PRP. In all the animals, a full-thickness 3x3 cm mesh skin graft was implanted. In the left limb, the biomaterial was place between the graft and the receptor bed, according to the group, while the right limb served as the control group. All the animals were evaluated clinically every 48 hours until the 14th day, using the variables of exudation, coloration, edema and cosmetic appearance. Three animals were evaluated histologically, on the third, seventh and tenth postoperative days, using the variables of fibroblasts, collagen, granulation tissue, microscopic integration-adherence and acute inflammation. RESULTS: Clinical evaluations showed that the group CF showed better scores for all variables compared to PRP group. On the histological evaluations PRP group had a higher presence of fibroblasts in the seventh and fourteenth days. CONCLUSION: The fibrin glue group was clinically superior to the platelet-rich group when used on full-thickness skin grafts in dogs.OBJETIVO: Comparar a cola de fibrina (Tissucol® e o plasma rico em plaquetas em enxertos cutâneos de espessura completa em malha em cães. MÉTODOS: Foram utilizados 18 cães, distribuídos em dois grupos, cola de fibrina (CF e plasma rico em plaquetas (PRP. Em todos os animais foi realizado um enxerto cutâneo de 3x3 cm, em malha de espessura completa. No membro esquerdo foi colocado o biomaterial entre o enxerto e o leito receptor, cada qual em seu grupo, o membro direito serviu como grupo controle. Todos os animais foram avaliados clinicamente a cada 48 horas até o décimo quarto dia, através das variáveis: exsudação, coloração, edema e aspecto cosmético; histologicamente em três animais, no terceiro, sétimo e décimo quarto dia de pós-operatório através das

  14. Investigation of production and influential factors of platelet-rich fibrin%富血小板纤维蛋白体外释放VEGF影响因素的探讨

    Institute of Scientific and Technical Information of China (English)

    李龙; 赵建辉; 刘斌; 令狐大科; 刘彦普; 梁丽华; 杨勇; 杨涛

    2012-01-01

    Objective To investigate the influence factors of platelet -rich fibrin (PRF) releasing vascular endothelialgrowth factor (VEGF) with two different kinds of collection tubes (dry glass tubes and plastic tubes) and with differentstorage temperature (4 degree and 37degree). Methods PRF was produced with two different kinds of collection tubes(dry glass tubes and plastic tubes) from healthy rabbit's medium-sized artery of the ears, and saved in different storagetemperature (4 degree and 37degree ) to test the quantity of released VEGF,and it was tested at seven experimentaltimes: 20 min, 4 h, 24 h (day 1),72h (day 3),120 h (day 5) and 168 h (day 7),0-168h(7days) with ELISA kit. ResultsThe total quantity of VEGF with dry glass tubes was higher than that with plastic tubes, in the temperature group itshowed that the total quantity of VEGF saved in 4 degree was higher than in the 37 degree. Conclusion Different kindsof collection tubes and different storage temperatures may affect the characteristics of PRF.The production of PRFshould use dry glass tubes and PRF should be saved at 4 degree before it was used.%目的:探讨不同材质的离心管和储存温度对制备的富血小板纤维蛋白(platelet-rich fibrin,PRF)释放血管内皮生长因子(vascular endothelial growth factor,VEGF)的影响.方法:分别用玻璃离心管和塑料离心管从健康家兔耳中动脉采血制备PRF,制备成PRF膜片后,加入无菌DMEM培养液各分为两组置于37℃和4℃条件下,并分别从 20min,4h,24h,72h,120h,168h,0~168h七个时间点收集PRF样本,用ELISA试剂盒检测各时间点VEGF的含量情况.结果:4℃条件下保存的PRF膜片释放VEGF含量高于37℃,玻璃离心管制备的PRF释放的VEGF含量高于塑料离心管.结论:不同的材质离心管和不同的储藏温度对PRF的特性存在一定影响,PRF的制备宜用玻璃离心管,在使用前宜储藏于4℃条件下.

  15. Comparative study on platelet-rich fibrin and platelet-rich plasma in repair of rabbit parietal bone defect%富血小板纤维蛋白与富血小板血浆修复兔颅顶骨缺损的比较研究

    Institute of Scientific and Technical Information of China (English)

    何通文; 韩耀辉; 牟兰; 徐庚池; 师爱萍; 葛振林

    2014-01-01

    目的 比较富血小板纤维蛋白(platelet-rich fibrin,PRF)与富血小板血浆(platelet-rich plasma,PRP)的成骨效果,探讨应用PRF修复骨缺损的方法. 方法 选择16只新西兰大白兔,在每只大白兔的颅顶骨上各制备4个直径为7 mm的骨缺损区,分为A、B、C、D区,然后根据填人材料的不同进行分组:A区填入PRF(PRF组)、B区填入PRF可吸收骨材料Bio-Oss骨粉(B0)(PRF+ BO组)、C区填入PRP+ BO(PRP+ BO组),D区填入PRP(PRP组),每组4只,术后2,4,8,12周分别行大体观察、X线片及组织学观察缺损区变化;并进行缺损区骨密度和新生骨小梁面积分析(均以灰度值表示). 结果 2周各缺损区已出现骨修复;4,8,12周各组缺损区骨修复效果均依次向好,其中B区最好,其次C区,A区好于D区.术后2周,A、B、C、D区骨小梁面积分别为10.95 ±0.58,15.45 ±0.79,10.22 ±0.43,6.58±0.64,任意两组比较差异均有统计学意义(F=22.869,P<0.01);术后4,8周,各组骨小梁面积均增加;12周时,A、B、C、D区骨小梁面积依次为35.09±0.58,59.44 ±0.60,50.75±1.56,30.94±1.19,任意两组比较差异均有统计学意义(F=1 002.904,P<0.01),结果显示,骨修复效果B区最好,其次C区,A区好于D区. 结论 PRF的成骨效果优于PRP,并且PRF复合BO修复骨缺损效果更好.%Objective To compare the osteogenesis effect of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) and investigate the methods of repairing bone defect with PRF.Methods Four defects measuring 7 mm in diameter were made in the parietal bone of 16 New Zealand white rabbits.The defects named A,B,C,and D and were filled with PRF,PRF-mixed Bio-Oss (BO),PRP-mixed BO,and PRP separately.Every four rabbits were sacrificed at postoperative 2,4,8,and 12 weeks and defects were examined grossly,radiographically,and histologically.Besides,bone mineral density and bone trabecular area were determined and expressed as gray-scale values.Results Newly regenerated bone appeared

  16. 微针导入自体富血小板血浆对面部年轻化的疗效研究%Effect of delivery autologous platelet rich plasma into skin with microneedle for facial rejuvenation

    Institute of Scientific and Technical Information of China (English)

    余晶; 冯幼平; 吴毅平

    2015-01-01

    Objective To observe the clinical effect of delivering autologous platelet rich plasma into skin with microneedles for facial rejuvenation. Methods 18 cases volunteers were treated for facial rejuvenation by delivery autologous platelet rich plasma into skin through micropores which the microneedle made.The 2ml autologous platelet rich plasma was extracted from 16ml whole blood by centrifuge.One session every 30 days,all 18 volunteers were treated 3 times.We assess its effect by questionaires for subjective evaluation,and objective evalation with digital skin analyzers to record the raw pictures and data before and after treatment. Results All 18 volunteers were obsevered significantimprovement of facial skin scores,miosture,elasticity,complexion,olein(P<0.05). Conclusion It is efficient to improve faciacal skin texture by delivering autologous Platelet Rich Plasma into skin with microneedle,it is an alternative for facial rejuvenation.%目的:探讨经微针皮肤导入自体富血小板血浆对面部年轻化的疗效与安全性.方法:对18例健康志愿者,抽取16ml静脉血,通过2次离心后得到约2.0ml自体富血小板血浆;采用电动纳米微针在面部逐点振动点刺,同时将自体富血小板血浆滴注到皮肤,通过微针制造的针孔导入皮肤组织内发挥疗效.每30d重复一次,3个月为一疗程,每次治疗前后采用MC760数字皮肤测试仪测试面部皮肤,比较皮肤水分、弹性、色斑、油脂等变化,通过皮肤状态问卷评估志愿者对治疗的满意度.结果:18例志愿者面部皮肤水分、弹性、色斑、油脂等指标和主观评分在治疗前后,自身对照有明显改善(P<0.05),无并发症发生.结论:使用微针导入自体富血小板血浆对面部年轻化治疗的疗效,为面部年轻化治疗提供了新的方法.

  17. Treatment of life-threatening wounds with a combination of allogenic platelet-rich plasma, fibrin glue and collagen matrix, and a literature review.

    Science.gov (United States)

    Asadi, Mehdi; Alamdari, Daryoush Hamidi; Rahimi, Hamid Reza; Aliakbarian, Mohsen; Jangjoo, Ali; Abdollahi, Abbas; Bahar, Mostafa Mehrabi; Azadmand, Ali; Forghani, Naser; Sadegh, Mohammad Nori; Khayamy, Mohammad Esmail; Seifalian, Alexander

    2014-08-01

    Currently there is no ideal procedure for the treatment of recalcitrant ulcers that are unresponsive to the majority of common treatments. However, several novel approaches have been proposed, including bone marrow stem cells, platelets, fibrin glue and collagen matrix. For the first approach treatment of a chronic wound, a non-invasive method is highly desirable. The present study was undertaken with the aim of evaluating the effect of a combination of platelets, fibrin glue and collagen matrix (PFC) in one treatment. A total of ten patients with aggressive, refractory, life-threatening wounds were recruited for the study and their treatment effects were evaluated. Initially, the ulcers were extensively debrided, measured and photographed at weekly intervals. The PFC combination was applied topically to the wound every two days. Following treatment, the wound was completely closed in nine patients and was markedly reduced in the other patient. The mean 100% healing time for the nine patients was 11.3±5.22 weeks. There was no evidence of local or systemic complications or any abnormal tissue formation, keloid or hypertrophic scarring. Therefore, the results of the present study indicate that in the first approach, the combination of PFC components may be used safely in order to synergize the effect of chronic wound healing.

  18. Treatment of non-healing sternum wound after open-heart surgery with allogenic platelet-rich plasma and fibrin glue-preliminary outcomes

    Directory of Open Access Journals (Sweden)

    Mohammad Abbasi Tashnizi

    2013-01-01

    Full Text Available Introduction: Non-healing wound in the sternal region after coronary arteries bypass graft surgery is a serious complication. For healing a chronic wound, several novel approaches have been proposed recently such as using bone marrow stem cells, platelets and fibrin glue (PFG; but a non-invasive method is highly desirable in the first approach for treatment. The current study was undertaken to evaluate the effect of the combination of PFG in one treatment. Materials and Methods: We report on the treatment of six patients with life-threatening chronic sternum wounds, which caused septicemia with multi-drug resistant pathogens. The ulcers were extensively debrided initially and were measured and photographed at weekly intervals. The combination of PFG was applied topically on the wound after every 2 days. Results: The wounds were completely closed in five patients and significantly reduced in size in one. There was no evidence of local or systemic complications and any abnormal tissue formation, keloid or hypertrophic scarring. Conclusions: Our study suggests, in the first approach, PFG can be used safely in order to heal a non healing sternum wound following coronary artery bypass surgery.

  19. Posterolateral arthrodesis in lumbar spine surgery using autologous platelet-rich plasma and cancellous bone substitute: an osteoinductive and osteoconductive effect.

    Science.gov (United States)

    Tarantino, Roberto; Donnarumma, Pasquale; Mancarella, Cristina; Rullo, Marika; Ferrazza, Giancarlo; Barrella, Gianna; Martini, Sergio; Delfini, Roberto

    2014-08-01

    Study Design Prospective cohort study. Objectives To analyze the effectiveness and practicality of using cancellous bone substitute with platelet-rich plasma (PRP) in posterolateral arthrodesis. Methods Twenty consecutive patients underwent posterolateral arthrodesis with implantation of cancellous bone substitute soaked with PRP obtained directly in the operating theater on the right hemifield and cancellous bone substitute soaked with saline solution on the right. Results Computed tomography scans at 6 and 12 months after surgery were performed in all patients. Bone density was investigated by comparative analysis of region of interest. The data were analyzed with repeated-measures variance analyses with value of density after 6 months and value of density after 12 months, using age, levels of arthrodesis, and platelet count as covariates. The data demonstrated increased bone density using PRP and heterologous cancellous block resulting in an enhanced fusion rate during the first 6 months after surgery. Conclusions PRP used with cancellous bone substitute increases the rate of fusion and bone density joining osteoinductive and osteoconductive effect.

  20. Sports medicine and platelet-rich plasma: nonsurgical therapy.

    Science.gov (United States)

    Grambart, Sean T

    2015-01-01

    A Cochrane Review was performed to assess the effects of platelet-rich therapies for treating musculoskeletal soft tissue injuries. Selection criteria were randomized and quasirandomized controlled trials (RCTs) that compared platelet-rich therapy with either placebo, autologous whole blood, dry needling, or no platelet-rich therapy for people with acute or chronic musculoskeletal soft tissue injuries. Primary outcomes were functional status, pain, and adverse effects. The investigators found 19 studies that compared platelet-rich therapy with placebo, autologous whole blood, dry needling, or no platelet-rich therapy. Disorders included rotator cuff tears (arthroscopic repair; 6 trials); shoulder impingement syndrome surgery (1 trial); elbow epicondylitis (3 trials); anterior cruciate ligament (ACL) reconstruction (4 trials), ACL reconstruction (donor graft site application; 2 trials), patellar tendinopathy (1 trial), Achilles tendinopathy (1 trial), and acute Achilles rupture surgical repair (1 trial). They further subdivided the studies based on type of treatment, including tendinopathies in which platelet-rich therapy injections were the main treatment (5 trials), and surgical augmentation procedures in which platelet-rich therapy was applied during surgery (14 trials). The conclusion was that there is currently insufficient evidence to support the use of platelet-rich therapy for treating musculoskeletal soft tissue injuries. Researchers contemplating RCTs should consider the coverage of currently ongoing trials when assessing the need for future RCTs on specific conditions. There is a need for standardization of PRP preparation methods. At this time, the use of PRP in foot and ankle surgery as an orthobiologic does not have an absolute indication. Many of the studies are lower evidence-based from surgical techniques. Several in vitro studies have shown that growth factors promote the regeneration of bone, cartilage, and tendons. More clinical studies are

  1. PRF治疗牙龈退缩的对照研究%Controlled clinical study of treatment of gingival recession using Platelet-Rich-Fibrin (PRF) membranes with connective tissue graft

    Institute of Scientific and Technical Information of China (English)

    裴延平; 林松杉; 章禾

    2016-01-01

    Objective:To provide the Clinical evaluation of Choukroun's platelet-Rich-Fibrin (PRF) Membranes in treatment of gingival recessions contrasting connective tissue graft. Method:Choose 13 patients of Gingival Recessions in-tend to surgical treatment, measure periodontal pocket depth, width of attached gingival and loss of attachment level in pre-operative and postoperative 3 months. TO contrast the Clinical effect of Choukroun's PRF Membranes in Gingival Reces-sions. Result:It has no statistically significant change in Periodontal pocket depth by surgery for both groups (P>0.05);The width keratinized gingival and vertical gingival recession depth are increasing after operation, have statistically significant changes compared with pre-operation (P0.05);It was fewer for the PRF group in comparison with the control group for vertical gingival recession depth(P<0.05).Conclusion:For root coverage, it can be achieved a better clinical effects by Platelet-Rich-Fibrin (PRF) membranes ,especially for Miller ClassⅠorⅡ.%目的:通过对比观察采用Choukroun's富血小板纤维蛋白膜(PRF膜)和采用结缔组织瓣治疗牙龈退缩的临床效果。方法:临床上选取有牙龈退缩且有意愿进行手术治疗的患者13例,分为PRF膜组和对照组,分别实施手术治疗,在术前、术后3个月测量牙周袋深度(PD)、角化龈宽度(KTW)以及牙龈退缩量,并对结果进行对照分析。结果:两组数据中牙周袋深度术前术后比较均无显著性差异(P≤0.05);角化龈宽度和牙龈退缩量术前术后比较均有显著性差异(P≥0.05)。角化龈宽度术后比较两组之间无显著性差异(P≥0.05);牙龈退缩量对照组小于PRF组(P≤0.05)。结论:PRF膜(富血小板纤维蛋白)治疗牙龈退缩的根面覆盖量虽不及结缔组织瓣+冠向复位组,但对于MillerⅠ°、Ⅱ°同样可以达到很好的根面覆盖效果;且术后角化龈宽度明显增加

  2. The influence of centrifugal speed on biological characteristics of platelet-rich fibrin%离心速度对富血小板纤维蛋白生物学特性的影响

    Institute of Scientific and Technical Information of China (English)

    程亚楠; 徐普; 毛秋华; 廖军; 唐盛玉

    2016-01-01

    目的 探讨离心速度的不同对制备的富血小板纤维蛋白(platelet-rich fibrin,PRF)结构、白细胞与血小板回收率及生长因子释放量的影响.方法 分别采用3种离心速度(2500rpm;3000rpm;4000rpm)制备PRF,使用组织学和扫描电镜观察PRF凝胶结构;血细胞分析仪检测白细胞、血小板浓度;酶联免疫吸附实验(enzyme linkedimmunosorbent assay,ELISA)检测TGF-β1、PDGF-AB的浓度.结果 3种PRF凝胶的主体结构为纤维蛋白聚集形成的立体网状结构,血小板与白细胞主要集中在PRF凝胶的白膜层.2500rpm与3000rpm制备的PRF,血小板与白细胞回收率及TGF-β1、PDGF-AB的释放量高于4000rpm (P<0.05),2500rpm与3000rpm间的差异无统计学意义(P>0.05).结论 离心速度的不同会在一定程度上影响PRF的生物学特性,2500rpm与3000rpm是制备PRF较为理性的转速.

  3. 富血小板血纤蛋白的生物组成和功能%Biological composition and function of platelet-rich fibrin

    Institute of Scientific and Technical Information of China (English)

    罗雯静; 赵静辉; 马珊珊; 孙千月; 郭天奇; 周延民

    2014-01-01

    富血小板血纤蛋白(PRF)制备简便,不需要加抗凝剂,无伦理道德争议及血液交叉感染。其立体三维结构使得PRF较富血小板血浆更坚实稳定。PRF为血管化的天然诱导物,其结构为免疫反应提供了天然支持,会影响上皮细胞和成纤维细胞的新陈代谢。其内的白细胞通过释放高质量分数的促炎因子白细胞介素-1β、4、6以及肿瘤坏死因子α和血管内皮生长因子,在抗感染、促组织愈合过程中起着重要的作用。PRF具有良好的骨诱导性,在即刻种植中将其用于辅助上颌窦提升,上颌窦骨密度和骨增量良好。PRF有利于骨髓间质干细胞的早期增殖和向成骨细胞的早期分化,明显促进人成骨细胞成骨相关有机基质分泌,促进成骨细胞早期成骨,抑制破骨细胞活化。本文就PRF的生物组成和功能,即诱导成骨、促软组织修复、促血管化和抗感染等研究进展作一综述。%Platelet-rich fibrin(PRF) has many advantages, such as easy preparation with no anticoagulant, no ethical controversy, and no blood cross-infection. Moreover, its three-dimensional structure makes PRF more solid and stable than platelet-rich plasma. PRF serves as a natural inducer of angiogenesis, and its structure provides natural support for immune responses. PRF can influence the metabolism of epithelial cells and fibroblasts. Leukocytes within PRF have important functions in resistance to infection and promotion of the tissue healing process by releasing high mass fractions of pro-inflammatory cytokines, including interleukin-1β, 4, 6, tumor necrosis factorα, and vascular endothelial growth factor. PRF exhibits good properties of osteoinduction, which is used to assist maxillary sinus lift

  4. Platelet-Rich Blood Derivatives for Stem Cell-Based Tissue Engineering and Regeneration

    NARCIS (Netherlands)

    Masoudi, E.A.; Ribas, J.; Kaushik, G.; Leijten, J.C.H.; Khademhosseini, A.

    2016-01-01

    Platelet-rich blood derivatives have been widely used in different fields of medicine and stem cell-based tissue engineering. They represent natural cocktails of autologous growth factors, which could provide an alternative for recombinant protein-based approaches. Platelet-rich blood derivatives, s

  5. 多孔羟基磷灰石与富血小板血浆和纤维蛋白胶复合修复骨缺损★%Compound of porous hydroxyapatite, platelet-rich plasma and fibrin glue in the treatment of bone defects

    Institute of Scientific and Technical Information of China (English)

    刘东光; 韦正超; 蔡道章; 郑军; 陈环球; 赖欢乐

    2013-01-01

      BACKGROUND: Autogenous bone graft is the best way to treat bone defects, but its limited sources and donor site complications restrain its clinical application. Therefore, to develop a substitute material has been a hotspot in the orthopedics. OBJECTIVE: To investigate the feasibility and effectiveness of the compound of coral ine hydroxyapatite porous, platelet-rich plasma and fibrin glue acting as a bone substitute material for repair of bone defects. METHODS: 1.5 cm radial segments from the bilateral forearms of New Zealand white rabbits were removed to prepare bone defect models. Then, bone defect models were randomly divided into a compound group (coral ine hydroxyapatite porous, platelet-rich plasma and fibrin glue), control group treated with autogenous bone graft, and blank group without implantation. RESULTS AND CONCLUSION: (1) X-ray observation: Bone defects were repaired completely in the compound group til the end of postoperative week 12, appearing with complete plasticity. The healing process in the compound group was synchronized with that in the control group. However, there were no changes in bone defects of the blank group. (2) Histopathological examination: Bone repair basical y completed in the compound and control groups at week 12 after operation, presenting with mature lamel ar bone and Haversian canals. In the blank group, only a great amount of fibroblasts proliferated, but no bone formed. (3) Biomechanical analysis: The maximum torque and torsional stiffness of the compound group were better than those of the control group at 2 weeks postoperation (P < 0.05). However, there was no significant difference between the maximum torque and torsional stiffness of the compound and control groups. These results demonstrate that the compound of coral ine hydroxyapatite porous, platelet-rich plasma and fibrin glue acting as a bone substitute is superior to autologous bone in the early repair of bone defects, which is beneficial to bone healing

  6. Effects of crypreservation on platelet-rich fibrin%冷冻保存对富血小板纤维蛋白组织形态与生物活性因子的影响

    Institute of Scientific and Technical Information of China (English)

    李丹; 张剑明; 罗晓丁; 韩哲

    2012-01-01

    目的 观察超低温冷冻保存方法对富血小板纤维蛋白(PRF)组织形态与生物活性因子的影响.方法 取健康志愿者的肘静脉血提取PRF标本,液氮保存1周,分别进行光镜、透射电镜观察和免疫组化分析,并与新鲜PRF对比.结果 光镜下冷冻前后的PRF无明显差异,免疫组化检测冷冻前后血小板源性生长因子(PDGF)、转化生长因子β1 (TGF-β1)、白细胞介素-1(IL-1)、IL-4、肿瘤坏死因子(TNF)均有阳性反应表达,其中PDGF、TGF-β1、IL-1、TNF表达差异无统计学意义(P>0.05),冻后PRF中IL-4表达高于新鲜PRF,差异有统计学意义[平均光密度值(0.207±0.006)比(0.125±0.015),P<0.05].透射电镜显示,冻后PRF中大部分血小板外形不规则或有膜损伤.结论 冷冻保存对PRF生物活性因子和纤维蛋白结构无明显影响,仍可保持其促进组织愈合和抗炎反应的作用.%Objective To investigate the effects of cryopreservation on the morphology and bioactive factors of platelet-rich fibrin(PRF).Methods PRF was carried out at - 196 ℃ for one week and investigated by light microscope.Transmission electron microscope (TEM) and the bioactive factors in PRF were analyzed before and after the cryopreservation.Results PRF had no significant changes in light microscope before and after cryopreservation.The immuno-histochemical results showed that Platelet derived growth factor (PDGF),transforming growth factor beta 1 ( TGF beta 1 ),interleukin 1 ( IL-1,IL-4) and tumor necrosis factor (TNF) were expressed both in fresh and frozen PRF.The express of PDGF,TGF-β1,IL-1,TNF in frozen PRF was not significantly increased compared with fresh PRF( P > 0.05 ),while the express of IL-4 in frozen PRF increased significantly( P < 0.05 ).Conclusion The fibrin net-structure and bioactive factors of frozen PRF are not significantly destroyed by cryopreservation treatment.

  7. Effects of platelet-rich fibrin on canine dental pulp cells in vitro%富血小板纤维蛋白对犬牙髓细胞的体外作用

    Institute of Scientific and Technical Information of China (English)

    杨盼盼; 战园; 李盛林; 刘鹤

    2013-01-01

    目的:检测比格犬静脉血制取的富血小板纤维蛋白(platelet-rich fibrin,PRF)对自体牙髓细胞(canine dental pulp cells,cDPCs)增殖和趋化的作用,探讨PRF作为自体来源生物材料在临床活髓治疗中应用的可行性.方法:用酶消化法分离培养cDPCs;用Choukroun一步离心法制取PRF,将其浸泡于纯净的最小必需培养基α(minimum essential medium alpha medium,α-MEM)中,于第7天取浸出液,即为PRF浸出液.细胞增殖作用采用细胞计数试剂盒-8(cell counting kit-8,CCK-8)检测,对照组为含2%(体积分数)胎牛血清(fetal bovine serum,FBS)的α-MEM培养基,实验组为含2% FBS的PRF浸出液,并按PRF浸出液浓度(体积分数)分为20%、40%、60%、80%、100%共5组,分别记为PRF1、PRF2、PRF3、PRF4、PRF5.趋化实验采用 Transwell模型,实验组PRF浸出液浓度选择对增殖促进作用最显著的浓度,阴性对照组为不含FBS的α-MEM培养基,阳性对照组为含30%(体积分数)FBS的α-MEM培养基,各组上室均接种1×105个细胞.结果:PRF2组的光密度值(1.45±0.06)显著高于对照组(1.21±0.11),差异具有统计学意义(P<0.001),PRF1组、PRF3组、PRF4组、PRF5组光密度值分别为1.20±0.02、1.28±0.04、1.19±0.02、1.22±0.02,与对照组差异无统计学意义(P值分别为0.902、0.084、0.726、0.779),即40%浓度的PRF浸出液对自体cDPCs的增殖具有显著的促进作用,在该浓度下,PRF组细胞迁移数目为55.89±18.42,与阴性对照组(6.52±1.97)比较差异具有统计学意义(P<0.001),而与阳性对照组(59.25±29.17)差异无统计学意义(P=0.970).结论:PRF与cDPCs有良好的生物相容性,40%浓度的PRF浸出液可促进cDPCs的增殖、趋化作用,提示PRF可作为活髓治疗中牙髓修复的盖髓材料使用.

  8. 自体PRP/PRF脂肪颗粒移植面部填充的回顾性临床分析%Retrospective analysis of facial filling with autologous platelet-rich plasma or platelet-rich fibrin

    Institute of Scientific and Technical Information of China (English)

    高伟成; 陈小平; 林金德; 王昕; 尹康; 胡晓庆

    2015-01-01

    目的 评价自体脂肪颗粒联合富血小板血浆或富血小板纤维蛋白进行面部填充的临床疗效.方法 141例面部局部凹陷的患者,63例采用富血小板血浆脂肪颗粒移植填充,78例采用富血小板纤维蛋白脂肪颗粒移植填充.术后随访3个月至3年,对术后是否行二次填充和满意度进行评价.结果 141例患者均未出现感染、脂肪液化、血肿、纤维囊性化、色素沉着、感觉迟钝、局部凹凸不平等并发症.2例富血小板血浆脂肪颗粒移植患者注射1周后受区局部出现小片红斑,1周后好转;2例富血小板血浆脂肪颗粒移植患者和l例富血小板纤维蛋白脂肪颗粒移植患者术后受区局部出现小团块,经局部按摩或局部细针穿刺抽取后肿块消失.富血小板血浆脂肪颗粒移植患者二次填充率为26.9%,富血小板纤维蛋白脂肪颗粒移植患者二次填充率为l0.2%.结论 自体脂肪颗粒联合富血小板血浆或富血小板纤维蛋白移植是治疗面部凹陷的有效方法.与富血小板血浆相比,富血小板纤维蛋白在临床应用更方便,并发症更少,中、远期效果较好,值得推广.

  9. Clinical Applications of Platelet-Rich Plasma in Patellar Tendinopathy

    OpenAIRE

    D. U. Jeong; C.-R. Lee; Lee, J.H.; Pak, J.; L.-W. Kang; B. C. Jeong; Lee, S. H.

    2014-01-01

    Platelet-rich plasma (PRP), a blood derivative with high concentrations of platelets, has been found to have high levels of autologous growth factors (GFs), such as transforming growth factor-β (TGF-β), platelet-derived growth factor (PDGF), fibroblastic growth factor (FGF), vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF). These GFs and other biological active proteins of PRP can promote tissue healing through the regulation of fibrosis and angiogenesis. Moreover,...

  10. Use of Platelet Rich Plasma Gel on Wound Healing: A Systematic Review and Meta-Analysis

    OpenAIRE

    Marissa J Carter; Fylling, Carelyn P.; Parnell, Laura K.S.

    2011-01-01

    Objective: Autologous platelet rich plasma is an advanced wound therapy used in hard-to-heal acute and chronic wounds. To better understand the use and clinical outcomes of the therapy, a systematic review of the published literature in cutaneous wounds was performed. Methods: Electronic and hand searches for randomized controlled trials and comparative group studies using platelet rich plasma therapy in cutaneous wounds and published over the last 10 years was conducted. Eligible studies com...

  11. Differentiation within autologous fibrin scaffolds of porcine dermal cells with the mesenchymal stem cell phenotype

    Energy Technology Data Exchange (ETDEWEB)

    Puente, Pilar de la, E-mail: pilardelapuentegarcia@gmail.com [Tissue Bank, San Francisco Clinic Foundation, Av./Facultad 51, 5°, 24004 León (Spain); Ludeña, Dolores [Pathology Service, University Hospital of Salamanca, P/San Vicente 58-182, 37007 Salamanca (Spain); López, Marta; Ramos, Jennifer; Iglesias, Javier [Tissue Bank, San Francisco Clinic Foundation, Av./Facultad 51, 5°, 24004 León (Spain)

    2013-02-01

    Porcine mesenchymal stem cells (pMSCs) are an attractive source of cells for tissue engineering because their properties are similar to those of human stem cells. pMSCs can be found in different tissues but their dermal origin has not been studied in depth. Additionally, MSCs differentiation in monolayer cultures requires subcultured cells, and these cells are at risk of dedifferentiation when implanting them into living tissue. Following this, we attempted to characterize the MSCs phenotype of porcine dermal cells and to evaluate their cellular proliferation and differentiation in autologous fibrin scaffolds (AFSs). Dermal biopsies and blood samples were obtained from 12 pigs. Dermal cells were characterized by flow cytometry. Frozen autologous plasma was used to prepare AFSs. pMSC differentiation was studied in standard structures (monolayers and pellets) and in AFSs. The pMSCs expressed the CD90 and CD29 markers of the mesenchymal lineage. AFSs afforded adipogenic, osteogenic and chondrogenic differentiation. The porcine dermis can be proposed to be a good source of MSCs with adequate proliferative capacity and a suitable expression of markers. The pMSCs also showed optimal proliferation and differentiation in AFSs, such that these might serve as a promising autologous and implantable material for use in tissue engineering. -- Highlights: ► Low fibrinogen concentration provides a suitable matrix for cell migration and differentiation. ► Autologous fibrin scaffolds is a promising technique in tissue engineering. ► Dermal cells are an easily accessible mesenchymal stem cell source. ► Fibrin scaffolds afforded adipogenic, osteogenic and chondrogenic differentiation.

  12. 富血小板纤维蛋白与富血小板血浆体外释放生长因子的比较及其对脂肪干细胞增殖分化的影响%The comparison of platelet-rich fibrin and platelet-rich plasma in releasing of growth factors and their effects on the proliferation and differentiation of adipose tissue-derived stem cells in vitro

    Institute of Scientific and Technical Information of China (English)

    杨世茂; 王明国; 李静; 刘金盼; 林夏莲; 徐炜

    2012-01-01

    目的 比较富血小板纤维蛋白(PRF)与富血小板血浆(PRP)体外释放生长因子的质量浓度及其对脂肪干细胞(ADSCs)增殖和成骨分化的影响.方法 抽取兔耳中央动脉血,一次离心法制备PRF,二次离心法制备PRP,分别将其置于5 mL新鲜的α-MEM培养液中,分别于37℃下静置1、7、14、21、28 d,收集PRF与PRP析出液,检测析出液中转化生长因子-β1(TGF-β1)及血小板源性生长因子-AB(PDGF-AB)的质量浓度.将收集的PRF与PRP析出液配置成条件培养液培养ADSCs,观察其对ADSCs增殖及碱性磷酸酶(ALP)活性的影响.结果 1)生长因子释放情况:不同时间点的PRF析出液中,14d时TGF-β1的质量浓度达到最高,7d时PDGF-AB的质量浓度最高;不同时间点的PRP析出液中,1d时TGF-β1与PDGF-AB的质量浓度即达最高,以后逐渐下降.2)对ADSCs增殖及ALP活性的影响:PRF析出液中,14 d时对其影响最大;PRP析出液中,1d时对其影响最大.结论 与PRP相比,PRF能够缓慢持久地释放生长因子,更有力地刺激ADSCs的增殖和分化.%Objective To compare the releasing of growth factors between platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) as well as their effects on the proliferation and differentiation of adipose tissue-derived stem cells (ADSCs) in vitro. Methods Blood was taken from central artery of rabbits, acquiring PRF was acquired through one time centrifuge and PRP through two times centrifuge. Five milliliters of fresh α-MEM was added to PRF and PRP and incubated at 37 ℃. The time points to collect exudates was in day 1, 7, 14, 21, 28 and the mass concentrations of transforming growth factor-β1 (TGF-β1) and platelet derived growth faetor-AB (PDGF-AB) were quantified in PRF and PRP. Then the exudates of PRF and PRP were used to culture ADSCs and evaluate the effects of PRF and PRP on proliferation and differentiation of ADSCs. Results 1) Growth factor release: In the PRF exudates at different time points

  13. Platelet-Rich Plasma Combined With Skin Substitute for Chronic Wound Healing: A Case Report

    OpenAIRE

    Knox, Rebecca L.; Hunt, Allen R.; Collins, John C.; DeSmet, Marie; Barnes, Sara

    2006-01-01

    Contemporary management of chronic wounds focuses on improving natural healing and individualization of treatment. Incorporating multiple therapies has become increasingly common. Of interest are autologous growth factors, which are especially important in chronic wound healing and may contribute to tissue formation and epithelialization. Autologous platelet concentrate or platelet-rich plasma (PRP) is a concentration of at least five autologous growth factors and has been shown to accelerate...

  14. A novel approach to human cranial tissue regeneration and frontal sinus obliteration with an autogenous platelet-rich/fibrin-rich composite matrix: 10 patients with a 6-10 year follow-up.

    Science.gov (United States)

    Mendonça-Caridad, Jose; Lopez, Pedro Juiz; Fayos, Francisco Vidal; Miery, Gustavo

    2013-06-01

    Advanced frontal sinus disease non-responsive to conservative therapy has been treated with fat obliteration for decades. More recently, a wide variety of autogenous, allogenic or synthetic materials have also been used. In this study we present a treatment based on totally autogenous procedures and materials that was successfully implemented in 10 patients and followed up for a period of 6-10 years, to evaluate the feasibility of a new approach for the treatment of frontal sinus disease and other related cranial osseous derangements, based on regenerative medicine as an alternative to fat or other obliterating or grafting materials. Platelet-rich and -poor plasma (PRP, PPP) are set to clot with cortical shavings from the skull surface. After surgically stimulating the sinus to encourage cell chemotaxis, migration and homing, the bioactive scaffold is placed and covered with a PPP membrane and a periosteal flap. Ten patients with pathologies ranging from devastating infection to invasive tumours or trauma were treated with this regenerative procedure in a single-stage surgery. All patients had an uneventful recovery with bone formation and no complications or recurrences over the years. The application of modern principles in tissue regeneration and wound healing has resulted in a favourable outcome, with no complications or sequelae, in a series of 10 patients with advanced frontal sinus disease over a long period of time.

  15. 富血小板纤维蛋白体外释放TGF-β和PDGF-AB影响因素的探讨%Investigation of effect factors of TGF-β and PDGF-AB levels in platelet-rich fibrin releasing in vitro

    Institute of Scientific and Technical Information of China (English)

    李艳秋; 周延民; 孙晓琳; 张天首; 朱婷

    2012-01-01

    目的 探讨不同方法对制备的富血小板纤维蛋白(platelet-rich fibrin,PRF)释放生长因子的影响.方法 对志愿者进行静脉采血,分别以不同离心速度(2500r/min、3000r/min、3500r/min)、离心时间(10min、15min、20min)和除水方法(快速除水和缓慢除水)制备PRF,收集PRF所释放的生长因子,通过ELISA法比较不同方法所获得的PRF释放的TGF-β和PDGF-AB的浓度差异.结果 以3000r/min的离心速度制备的PRF释放TGF-β和PDGF-AB含量显著高于其他两组;10min组和15min组制备的PRF释放TGF-β含量高于20min组,而PDGF-AB含量10min组显著高于其他两组;两种除水方法制备的PRF释放TGF-β和PDGF-AB含量无统计学差异(P>0.05).结论 不同离心速度、离心时间对PRF的特性存在一定的影响,而快速除水和缓慢除水对PRF特性的影响无显著性差异.%Objective To investigate the influence factors of platelet-rich fibrin releasing growth factors with different kinds. Methods PRF were produced by different centrifugal speed (2500r/min、3000r/min、3500r/min) , centrifugal time (10min、15min、20min) and dehydration methods (fast dehydration methods and slow dehydration methods) from volunteers.Collect the growth factors in PRF ,and Compare the TGF-β and PDGF-AB concentration by ELISA. Results TGF-pand PDGF-AB levels in the PRF prepared by 3000r/min were higher ; TGF-filevel in the PRF prepared by 10min and 15min were higher, and PDGF-AB level in the PRF prepared by 10min were higher;but the two dehydration methods showed no statistics significance (R>0.05). Conclusion Different centrifugal speed and centrifugal time may affect the characteristics of PRF, and dehydration methods may not affect the characteristics of PRF.

  16. The basic science of platelet-rich plasma (PRP): what clinicians need to know.

    Science.gov (United States)

    Arnoczky, Steven P; Sheibani-Rad, Shahin; Shebani-Rad, Shahin

    2013-12-01

    Platelet-rich plasma (PRP) has been advocated for the biological augmentation of tissue healing and regeneration through the local introduction of increased levels (above baseline) of platelets and their associated bioactive molecules. In theory, the increased levels of autologous growth factors and secretory proteins provided by the concentrated platelets may enhance the wound healing process, especially in degenerative tissues or biologically compromised individuals. Although PRP has been increasingly utilized in the treatment of a variety of sports-related injuries, improvements in healing and clinical outcomes have not been universally reported. One reason for this may be the fact that all PRP preparations are not the same. Variations in the volume of whole blood taken, the platelet recovery efficacy, the final volume of plasma in which the platelets are suspended, and the presence or absence of white blood cells, and the addition of exogenous thrombin to activate the platelets or calcium chloride to induce fibrin formation, can all affect the character and potential efficacy of the final PRP product. This article will review the basic principles involved in creating PRP and examine the potential basic scientific significance of the individual blood components contained in the various forms of PRP currently used in sports medicine.

  17. Clinical Applications of Platelet-Rich Plasma in Patellar Tendinopathy

    Directory of Open Access Journals (Sweden)

    D. U. Jeong

    2014-01-01

    Full Text Available Platelet-rich plasma (PRP, a blood derivative with high concentrations of platelets, has been found to have high levels of autologous growth factors (GFs, such as transforming growth factor-β (TGF-β, platelet-derived growth factor (PDGF, fibroblastic growth factor (FGF, vascular endothelial growth factor (VEGF, and epidermal growth factor (EGF. These GFs and other biological active proteins of PRP can promote tissue healing through the regulation of fibrosis and angiogenesis. Moreover, PRP is considered to be safe due to its autologous nature and long-term usage without any reported major complications. Therefore, PRP therapy could be an option in treating overused tendon damage such as chronic tendinopathy. Here, we present a systematic review highlighting the clinical effectiveness of PRP injection therapy in patellar tendinopathy, which is a major cause of athletes to retire from their respective careers.

  18. Clinical applications of platelet-rich plasma in patellar tendinopathy.

    Science.gov (United States)

    Jeong, D U; Lee, C-R; Lee, J H; Pak, J; Kang, L-W; Jeong, B C; Lee, S H

    2014-01-01

    Platelet-rich plasma (PRP), a blood derivative with high concentrations of platelets, has been found to have high levels of autologous growth factors (GFs), such as transforming growth factor-β (TGF-β), platelet-derived growth factor (PDGF), fibroblastic growth factor (FGF), vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF). These GFs and other biological active proteins of PRP can promote tissue healing through the regulation of fibrosis and angiogenesis. Moreover, PRP is considered to be safe due to its autologous nature and long-term usage without any reported major complications. Therefore, PRP therapy could be an option in treating overused tendon damage such as chronic tendinopathy. Here, we present a systematic review highlighting the clinical effectiveness of PRP injection therapy in patellar tendinopathy, which is a major cause of athletes to retire from their respective careers. PMID:25136568

  19. Autologous fibrin glue as an encapsulating scaffold for delivery of retinal progenitor cells

    Directory of Open Access Journals (Sweden)

    Tamer Anwar Esmail Ahmed

    2015-02-01

    Full Text Available The retina is a highly sophisticated piece of the neural machinery that begins the translation of incoming light signals into meaningful visual information. Several degenerative diseases of the retina are characterized by photoreceptor loss and eventually lead to irreversible blindness. Regenerative medicine, using tissue engineering-based constructs to deliver progenitor cells or photoreceptors along with supporting carrier matrix is a promising approach for restoration of structure and function. Fresh fibrin glue (FG produced by the CryoSeal®FS system in combination with mouse retinal progenitor cells (RPCs were evaluated in this study. In vitro expanded RPCs isolated from postnatal mouse retina were encapsulated into FG and cultured in the presence of the protease inhibitor, tranexamic acid. Encapsulation of RPCs into FG did not show adverse effects on cell proliferation or cell survival. RPCs exhibited fibroblast-like morphology concomitantly with attachment to the encapsulating FG surface. They expressed α7 and β3 integrin subunits that could mediate attachment to fibrin matrix via an RGD independent mechanism. The three dimensional environment and the attachment surface provided by FG was associated with a rapid downregulation of the progenitor marker SOX2 and enhanced the expression of the differentiation markers CRX and recoverin. However, the in vitro culture conditions did not promote full differentiation into mature photoreceptors. Nevertheless, we have shown that autologous fibrin, when fabricated into a scaffold for RPCs for delivery to the retina, provides the cells with external cues that could potentially improve the differentiation events. Hence, transient encapsulation of RPCs into FG could be a valid and potential treatment strategy to promote retinal regeneration following degenerative diseases. However, further optimization is necessary to maximize the outcomes in terms of mature photoreceptors.

  20. Efficacy of platelet-rich plasma in treatment of androgenic alopecia

    OpenAIRE

    Parul Singhal; Sachin Agarwal; Paramjeet Singh Dhot; Sayal, Satish K.

    2015-01-01

    Background: Platelet-rich plasma (PRP) has shown remarkable beneficial effects without any major adverse reactions in the treatment of androgenic alopecia. The growth factors in activated autologous PRP induce the proliferation of dermal papilla cells. Objectives: The objective was to investigate the clinical efficacy of PRP in treatment of androgenic alopecia. Materials and Methods: Ten patients were given autologous PRP injections on the affected area of alopecia over a period of 3 months a...

  1. Platelet-rich plasma gel in combination with Schwann cells for repair of sciatic nerve injury☆

    OpenAIRE

    Ye, Fagang; Li, Haiyan; Qiao, Guangxi; Chen, Feng; Tao, Hao; Ji, Aiyu; Hu, Yanling

    2012-01-01

    Bone marrow mesenchymal stem cells were isolated from New Zealand white rabbits, culture-expanded and differentiated into Schwann cell-like cells. Autologous platelet-rich plasma and Schwann cell-like cells were mixed in suspension at a density of 1 × 106 cells/mL, prior to introduction into a poly (lactic-co-glycolic acid) conduit. Fabricated tissue-engineered nerves were implanted into rabbits to bridge 10 mm sciatic nerve defects (platelet-rich plasma group). Controls were established usin...

  2. The study of orthotopic transplantation by inactivated infection bone and autologous platelet-rich plasma combined with bone marrow on chronic osteomyelitis%感染骨灭活原位移植自体富血小板血浆复合骨髓治疗慢性骨髓炎

    Institute of Scientific and Technical Information of China (English)

    贝朝涌; 唐际存; 王锐英; 辛林伟; 魏凌云; 伍志海; 李强

    2012-01-01

    目的:评价感染骨灭活原位移植自体富血小板血浆复合骨髓治疗慢性骨髓炎的疗效.方法:40只新西兰大白兔,随机分成两组,制成慢性骨髓炎模型.以感染灶最严重处为中心,线锯截取出死骨及感染骨2.0 cm煮沸灭活15 min,原位移植并闭合伤口.实验组术后局部注射制备好的自体富血小板血浆复合骨髓悬液,隔周1次,连续注射3周.对照组注射生理盐水.分别于术后第4、8、12、16周处死5只动物,行X线观察、组织学观察.结果:实验组成骨修复X线评分、组织学评分均高于对照组,两组之间差异有显著性(P<0.05).结论:感染骨灭活原位移植可作为骨支架,自体富血小板血浆复合骨髓,具有抗感染及促进成骨的双重作用,治疗慢性骨髓炎效果良好.%Objective To evaluate the treatment effect of orthotopic transplantation by inactivated infection bone and autologous platelet-rich plasma combined with bone marrow on chronic osteomyelitis. Methods Forty New Zealand white rabbits were randomly divided into two groups. A model of chronic osteomyelitis was performed. At the center of the most serious foci, the sequestrum and infected bone (2.0 cm) were removed, which boiled for 15 minutes. Then the orthotopic transplantation was performed, the wound was closed. The treatment group was injected with autologous platelet-rich plasma combined with bone marrow suspension in local foci after the operation, once every other week, continuous 3 weeks. The control group was injected with normal saline. Five animals were sacrificed respectively after 4 , 8, 12, 16 weeks. The treatment effect was evaluated by radiographs and histological analysis. Results Compare with control group, osteoblast repaired X-ray scores, histological scores of bone repaired in the treatment group were significance increased. The result has statistical significance (P < 0.05).Conclusions Orthotopic transplantation of inactivated infection bone can be

  3. The effect of platelet-rich fibrin gel precipitate liquid on mineralization of human dental pulp cells in vitro%富血小板纤维蛋白凝胶析出液对人牙髓细胞体外矿化的影响

    Institute of Scientific and Technical Information of China (English)

    何璇; 韦维; 陈文霞

    2015-01-01

    目的:探索富血小板纤维蛋白(platelet-rich fibrin,PRF)凝胶析出液对人牙髓细胞(human dental pulp cells, hDPCs)体外矿化的影响。方法组织块法培养 hDPCs。采用 Choukroun 一步离心法制备 PRF 凝胶。将新鲜制备的PRF 凝胶浸泡于 DMEM 培养基中,于第7 d 取析出液。用 PRF 凝胶析出液孵育 hDPCs 3 d 后更换矿化诱导液。采用茜素红染色和 RT-PCR 检测人牙髓细胞矿化的潜能。结果矿化诱导21 d 后,茜素红染色观察到实验组有少量钙结节生成,而对照组无钙结节生成;RT-PCR 结果显示,实验组 hDPCs 碱性磷酸酶(ALP)的表达为对照组的1.5倍,差异具有统计学意义(P <0.05)。结论 PRF 凝胶析出液可促进人牙髓细胞矿化。%Objective This study was designed to investigate the effect of platelet-rich fibrin gel (PRF gel)precipitate liquid on the mineralization of human dental pulp cells (hDPCs)in vitro. Methods The hD-PCs were separated and cultured by using tissue block culture method.PRF gel was prepared by Choukroun's protocols.The newly prepared PRF gel was dipped in DMEM culture media,the precipitate liquid of PRF gel was collected on day 7.hDPCs were treated with mineralization induction solution 3 days after being incubated with the precipitate liquid of PRF gel.The capacity of mineralization was measured by using alizarin red stai-ning and RT-PCR. Results Twenty-one days after mineralization induction,a small amount of mineral-ized nodules on alizarin red staining were observed in experimental group while no mineralized nodule was ob-served in control group;RT-PCR revealed that the expression of alkaline phosphatase (ALP)in experimental group was 1.5 times higher than that in control group,comparison yielded statistical difference (P <0.05). Conclusion The precipitate liquid of PRF gel can accelerate the mineralization of hDPCs.

  4. Histological study on the impact of immediate implantation of Platelet Rich Fibrin on rabbit alveolarridge defect%PRF 植入兔拔牙创内的实验研究

    Institute of Scientific and Technical Information of China (English)

    王芳; 孙乐刚; 张惠芹

    2014-01-01

    Objective To explore the role of PRF to promote bone regeneration of the sockets and the role to remain alveolar bone . Methods ①Experimental animal model:24 rabbits were randomly divided into 4 groups(group A, group B, group C, group D), with one mandibular central incisors extracted .PRF was prepared by the autologous blood and implanted into the sockets .A, B,C group were respectively implanted with PRF/nano-HA, PRF,nano-HA composite graft, and D group was as control.Animals were killed respectively at 4, 12 weeks and samples were obtained for histological observation .②.Observation: Histological slides were made with decalcified , dehydrated and paraffin -embedded specimen ,and angiogenesis and osteosis were observed under microscope . Results In the 4th week after surgery , quantity and quality of new bone tissues group A >group B >group C >group D.At 12 weeks the group A was better than other groups .There is no significant difference between the group B and group C .But both of them were better than qroup D .Conclusions ①PRF can accelerate healing of the bone tissue .②PRF/nano-HA play a role on preserving the alveolar ridge after teeth extraction and may be used as a favourable material on alveolarridge defect .%目的:探讨PRF对拔牙窝的促进骨再生作用及牙槽嵴保存的影响。方法①实验动物模型:将24只新西兰大白兔随机分为4组( A、B、C、D组),每组6只,拔除一侧下颌切牙。抽取大白兔自体外周血液,离心后制成PRF。A组拔牙窝内植入PRF/nano-HA混合物;B组拔牙窝内植入PRF;C组拔牙窝内植入nano-HA;D组拔牙窝作为空白对照。分别于术后4周、12周处死动物,取材。②观察:将标本脱钙脱水石蜡包埋行组织学切片,显微镜下观察骨愈合情况。结果术后4周,拔牙窝内新生骨组织数量质量A组>B组>C组>D组(空白组)。术后12周,A组成骨质量最好,B、C组骨改建情况类似,均优

  5. A New Treatment of Musculoskeletal System Lesions: Platelet Rich Plasma

    Directory of Open Access Journals (Sweden)

    Bayram Kelle

    2012-08-01

    Full Text Available Platelet rich plasma (PRP is a treatment modality that is very popular in recent years. It is obtained by centrifuging autologous blood. Although it has been used for tendon lesions, it could also used for many other lesions. Vascularization of tendons is relatively insufficient and therefore the treatment is difficult. They tend to be chronic. Although there are other treatments methods, the results are not always satisfactory. There are no known side effects of PRP. However, the effectiveness is highly controversial. Some studies indicated that PRP is very effective, but some say that PRP is ineffective. In this review we try to analyze the effectiveness and usability of PRP. [Archives Medical Review Journal 2012; 21(4.000: 236-245

  6. 早期应用富血小板血浆凝胶对自体脂肪组织移植存活率的影响%Platelet-rich plasma fibrin glue in the early stage for survival rate of autogeneic fat graft

    Institute of Scientific and Technical Information of China (English)

    黎洪棉; 柳大烈; 吴涛; 赵培冉; 梁双武

    2011-01-01

    背景:自体颗粒脂肪组织填充广泛用于修复重建领域,移植后组织大量被吸收可严重影响远期效果.目的:观察早期应用富血小板血浆对自体移植脂肪组织存活率的影响.方法:取健康成年人腹部脂肪组织颗粒进行纯化,同时抽取少量静脉血,采用离心法提取自体富血小板血浆,利用纤维蛋白胶的物理特性制备含有富血小板血浆的脂肪组织复合移植物,在裸鼠背部中线两侧各分离一个腔隙,富血小板血浆组将脂肪组织颗粒-富血小板血浆凝胶随机注射入一侧腔隙深筋膜下,对侧仅注入脂肪组织颗粒作为对照组.结果与结论:移植后1个月和3个月,与对照组比较,富血小板血浆组移植脂肪局部的血管增生均较明显(P < 0.05),脂肪质量保持率均较高(P < 0.05);移植物脂肪细胞纤维坏死率均较低(P < 0.05).提示早期应用富血小板血浆凝胶可促进移植脂肪组织局部的血管再生,增加脂肪组织的质量保持率,减少脂肪移植后的纤维坏死程度.%BACKGROUND: Autogeneic fat is the ideal filling material in aesthetic and reconstructive surgery. However, the autogeneic fat graft produces serious impact on long-term effect because much fat would be absorbed after transplantation. Accordingly,difficulties in this field are how to increase the survival rate of autogeneic fat graft.OBJECTIVE: To investigate the effect of platelet-rich plasma (PRP) on the survival rate of autogeneic free grain fat grafts.METHODS: The fat was harvested from abdominal region of healthy adults and purified. At the same time, venous blood was taken from concurrent patients and PRP was prepared by centrifugation. The grafts were made using fibrin glue combined with PRP according to the physical characteristics of fibrin glue. A subdermal pocket was created in each side of the dorsal midline,the grafts which carried fat particle and PRP fibrin glue were randomly implanted below the deep fascia of

  7. Platelet Rich Plasma for Treating Chronic Tendinopathy

    OpenAIRE

    Kaux, Jean-François

    2016-01-01

    Tendinopathy is a major problem in medicine and sports traumatology. It is due, inter alia, to mechanical overload. It remains a challenge for the medical world to the extent that its frequent resistance to conventional treatments never promises the patient a favourable response following therapeutic management. The development of platelet-rich plasma (PRP) is a new hope when therapeutic treatments such as NSAIDs, corticosteroid injections, eccentric rehabilitation, shock waves, etc. have sho...

  8. Platelet rich plasma versus laser therapy in lateral epicondylitis of elbow

    OpenAIRE

    Gyaneshwar Tonk; Anish Kumar; Amit Gupta

    2014-01-01

    Background: Platelet rich plasma (PRP) extract has shown to be a general stimulation for repair and currently used widely in various sports injury. A prospective observational study was done to assess the efficacy of autologous PRP injection in lateral epicondylitis of elbow, and compare the result with low level laser therapy. Materials and Methods: The trial was conducted at a tertiary care center for a period of 2 years. Eighty-one patients with chronic lateral epicondylitis were divid...

  9. Effects of Choukroun′s Platelet-Rich Fibrin on the Osteogenic Differentiation of Dog Dental Pulp Cells in vitro%富血小板纤维蛋白对牙髓细胞增殖与分化的影响

    Institute of Scientific and Technical Information of China (English)

    于佳; 郝永明; 陆家瑜; 赵伟; 曹春花; 邹德荣

    2014-01-01

    目的:评估牙髓细胞在富血小板纤维蛋白(platelet-rich fibrin, PRF)存在下的体外增殖及成骨分化的能力,为PRF作为支架材料、牙髓细胞作为种子细胞构建组织工程骨,进行前期研究。方法:3月龄比格犬拔除乳磨牙获得乳牙牙髓细胞;恒牙牙髓细胞从16月龄成年比格犬磨牙获得。静脉取血离心10 min获得PRF。实验分4组:对照组(普通培养基不加入PRF);实验组A(普通培养基加入PRF);实验组B(成骨诱导培养基不加入PRF);实验组C(成骨诱导培养基加入PRF)。分别于1、4、7和11d测定细胞数量、MTT值、半定量碱性磷酸酶值、成骨相关基因Q-PCR值,并于21d测定钙结节吸光度值。结果:PRF是一种可以促进牙髓细胞增殖的,无毒性作用的纤维网状支架结构;细胞水平上PRF促进了两种细胞的成骨分化:钙结节以及碱性磷酸酶半定量数值都明显上调(P<0.05);基因水平上,4个时间点成骨相关基因的表达量都显著增加(P<0.05),且乳牙牙髓细胞的表现均优于恒牙牙髓细胞。结论:可使用PRF和牙髓细胞复合构建组织工程骨。%Objective:To evaluate the effects of choukroun's PRF (platelet-rich fibrin) on proliferation and differentiation of dental pulp cells in vitro and making a pre-feasibility assess hunting for the optimum scaffold. Methods:Pulp tissues of deciduous and permanent molars were harvested from 3-month and 16-month old beagle dogs respectively. Dental pulp cells were isolated and cloned. Jugular vein blood were collected from beagle dogs and centrifugation at 3 000 r/min for 10 minutes to obtain PRF. The experiments were divided into four groups:control group (ordinary medium without PRF), test group A ( ordinary medium with PRF), test group B (osteoinduction medium without PRF), test group C (added PRF in osteoinduction medium ). Cell counts and cytotoxicity tests, semi

  10. Experiencia clínica en el empleo de factores de crecimiento autólogos obtenidos de plasma rico en plaquetas Clinical experience related to the use of autologous platelet rich plasma

    Directory of Open Access Journals (Sweden)

    J. Montón Echeverría

    2007-09-01

    Full Text Available El presente artículo pretende exponer el estado actual del conocimiento en relación con el empleo del plasma rico en plaquetas autólogo en diversas indicaciones encuadradas en el marco de la Cirugía Plástica, Estética y Reparadora así como la experiencia clínica acumulada en nuestro Servicio en relación con el uso de este tratamiento durante el periodo comprendido entre el 1 de Enero de 2005 y el 30 de Junio de 2007 (30 meses. Las evidencias clínicas acumuladas hasta el momento indican que el empleo de esta terapia en la promoción de los fenómenos reparativos asociados a distintos procedimientos quirúrgicos plásticos, estéticos y reparadores induce los siguientes efectos: incremento de los procesos de reparación tisular de tejidos blandos y óseos, disminución de las tasas de infección postoperatoria, del dolor y de las pérdidas hemáticas. Basándonos en dicha información exponemos nuestra experiencia sobre un total de 151 casos que comprenden casos de úlceras vasculares, pies diabéticos, radiopatías, pérdidas de sustancia postraumáticas, casos complejos y reconstrucción mamaria. La evidencia clínica, no cuantificada, nos muestra una evolución claramente satisfactoria, comprobando desde una perspectiva clínica una clara mejoría de los fenómenos reparativos.This article intends to expose the state of the art related to the clinical use of autologous rich platelet plasma in different procedures which belong to the field of Plastic and Reconstructive Surgery. It is also referred our clinical experience related to this practice during a 30 month period (January 2005 - June 2007. Clinical evidence shows that this therapy has been able to promote both soft and bone tissue regeneration with a decrease in postoperative infection rates, pain and bleeding. Based upon this knowledge, our experience is related to 151 cases of vascular ulcers, diabetic feet, radiopathy, posttraumatic loss of substance, complex situations and

  11. Platelet-rich plasma in regenerative medicine

    Directory of Open Access Journals (Sweden)

    Guhta Ra Hara and Thaha Basu

    2014-01-01

    Full Text Available Platelet-rich plasma (PRP contains at least seven growth factors including epidermal, plateletderived, transforming, vascular endothelial, fibroblast, insulin-like and keratinocyte growth factor. The therapeutic effect of PRP occurs because of the high concentration of these growth factors compared with those found in normal plasma. In recent years, PRP is widely used across many clinical fields, especially in regenerative medicine. This review aimed at presenting an overview of the applications of PRP in regenerative medicine. The mechanisms of PRP effects on healing are also stated in this review. [Biomed Res Ther 2014; 1(1.000: 25-31

  12. The use of fibrin matrix-mixed gel-type autologous chondrocyte implantation in the treatment for osteochondral lesions of the talus

    OpenAIRE

    Lee, Kyung Tai; Kim, Jin Su; Young, Ki Won; Lee, Young Koo; Park, Young Uk; Kim, Yong Hoon; Cho, Hun ki

    2012-01-01

    Purpose This study assessed the clinical results and second-look arthroscopy after fibrin matrix-mixed gel-type autologous chondrocyte implantation to treat osteochondral lesions of the talus. Methods Chondrocytes were harvested from the cuboid surface of the calcaneus in 38 patients and cultured, and gel-type autologous chondrocyte implantation was performed with or without medial malleolar osteotomy. Preoperative American orthopedic foot and ankle society ankle-hind foot scores, visual anal...

  13. Platelet-Rich Plasma in a Patient with Cerebral Palsy

    OpenAIRE

    Alcaraz, Jesús; Oliver, Antonio; Sánchez, Juana María

    2015-01-01

    Patient: Male, 6 Final Diagnosis: Cerebral palsy secundary perinatal hypoxia Symptoms: Cognitive impairment • epilectic seizure Medication: Platelet rich plasma Clinical Procedure: Cognitive improvement with neuroestimulator and neuroregenerator power of platelet rich plasma injection Specialty: Hematology Objective: Unusual clinical course Background: The use of platelet-rich plasma is a now a common medical technique known as regenerative medicine, through power cell activation and differen...

  14. Treatment results of combined platelet-rich plasma and fat injection in patients with velopharyngeal insufficiency

    Directory of Open Access Journals (Sweden)

    Hamid Reza Fathi

    2013-10-01

    Full Text Available Background: Velopharyngeal insufficiency causes hypernasal vocal quality and can also result in audible nasal air emission and difficulty in producing pressure consonants. The resulting speech is often socially unacceptable and can be difficult to understand. Platelet-rich plasma is an autologous derivative of whole blood. Today, the importance of clinical use of Platelet-rich plasma in the plastic surgery is considered. This study was designed to evaluate the effectiveness of combined Platelet-rich plasma and fat injection in the treatment of velopharyngeal insufficiency. Methods: In this prospective clinicaltrial study, of 15 patients including 9 males and 6 females and aged 15-20 years with mild/ moderate velopharyngeal insufficiency who were injected with 5 mL of combined Platelet-rich plasma (1 mL and fat (4 mL in the front volume in the posterior pharyngeal wall in the pre-vertebral fascia under sedation. Speech samples were recorded by nasoendoscopy before the injection, and at 6 weeks and 6 months after the injection. Assessment of pathologic speech was done by speech therapist. Results: Velar displacement showed significantly increased at 6 weeks after the injection (P=0.049. Velopharyngeal gap disappeared in 60% of patients at 6 weeks after the injection (P=0.019. Lateral pharyngeal wall movement showed significantly increased in 73.3% of the patients at 6 months after the injection (P=0.04. After the treatment, aerodynamic assessment showed significantly decreased in nasal air escape during phonation and repeat the words (P<0.05. Assessment speech therapist showed significantly improve quality of phonation in these patients (P<0.05. Conclusion: It seems that, combined Platelet-rich plasma and fat injection lead to improve voice resonance and reduce nasal air escape in all treated cases. It can be a promising alternative to major procedures, such as velopharyngoplasties, for the treatment of mild/ moderate velopharyngeal insufficiency.

  15. Release kinetics of platelet-derived and plasma-derived growth factors from autologous plasma rich in growth factors.

    Science.gov (United States)

    Anitua, Eduardo; Zalduendo, Mari Mar; Alkhraisat, Mohammad Hamdan; Orive, Gorka

    2013-10-01

    Many studies have evaluated the biological effects of platelet rich plasma reporting the final outcomes on cell and tissues. However, few studies have dealt with the kinetics of growth factor delivery by plasma rich in growth factors. Venous blood was obtained from three healthy volunteers and processed with PRGF-Endoret technology to prepare autologous plasma rich in growth factors. The gel-like fibrin scaffolds were then incubated in triplicate, in a cell culture medium to monitor the release of PDGF-AB, VEGF, HGF and IGF-I during 8 days of incubation. A leukocyte-platelet rich plasma was prepared employing the same technology and the concentrations of growth factors and interleukin-1β were determined after 24h of incubation. After each period, the medium was collected, fibrin clot was destroyed and the supernatants were stored at -80°C until analysis. The growth factor delivery is diffusion controlled with a rapid initial release by 30% of the bioactive content after 1h of incubation and a steady state release when almost 70% of the growth factor content has been delivered. Autologous fibrin matrix retained almost 30% of the amount of the growth factors after 8 days of incubation. The addition of leukocytes to the formula of platelet rich plasma did not increase the concentration of the growth factors, while it drastically increased the presence of pro-inflammatory IL-1β. Further studies employing an in vitro inflammatory model would be interesting to study the difference in growth factors and pro-inflammatory cytokines between leukocyte-free and leukocyte-rich platelet rich plasma.

  16. Autologous platelet gel and fibrin sealant enhance the efficacy of total knee arthroplasty : improved range of motion, decreased length of stay and a reduced incidence of arthrofibrosis

    NARCIS (Netherlands)

    Everts, Peter A. M.; Devilee, Roger J. J.; Oosterbos, Cornelis J. M.; Mahoney, Christine Brown; Schattenkerk, Maarten Eeftinck; Knape, Johannes T. A.; van Zundert, Andre

    2007-01-01

    In this study we describe the potential role of autologous platelet gel and fibrin sealant in unilateral total knee arthroplasty to improve the postoperative range of motion and to reduce the incidence of arthrofibrosis. Total knee arthroplasty is often associated with a considerable amount of post-

  17. Effect of platelet-rich fibrin compounded with osteoinduction active material on extraction site preservation%骨诱导活性材料复合富血小板纤维蛋白在拔牙位点保存中的作用

    Institute of Scientific and Technical Information of China (English)

    李一鸣; 尼加提·吐尔逊; 周晶; 李淑慧; 吴佩玲

    2015-01-01

    目的 探讨骨诱导活性材料(osteoinduction active material,OAM)复合富血小板纤维蛋白(platelet-rich fibrin,PRF)进行拔牙位点保存的修复效果.方法 12个月龄雄性beagle犬6只,微创拔除其上、下颌双侧第二侧切牙,将4个位点分为4组,分别为PRF+OAM组、PRF组、OAM组和对照组,分别填入PRF+OAM、PRF、OAM和不做处理.6只犬随机分别于术后4、12周各处死3只,取牙槽骨标本行形态学测量和组织学观察,采用锥形束CT(cone beam CT,CBCT)测量骨密度评价拔牙窝的愈合情况.结果 4、12周时PRF+OAM组唇舌侧牙槽嵴宽度((-0.08±0.02)、(-0.09±0.02)mm)和高度((-0.53±0.07)、(-1.15±0.07)mm)均明显低于PRF组(牙槽嵴宽度(-0.15±0.03)、(-0.63±0.04)mm)和高度((-0.74±0.06)、(-1.51±0.05)mm)、OAM组(牙槽嵴宽度(-0.22±0.03)、(-0.08±0.05)mm和高度((-0.95±0.07)、(-1.51±0.06)mm)及对照组(牙槽嵴宽度(-0.35±0.08)、(-1.17±0.21)mm和高度((-1.27±0.12)、(-1.70±0.05)mm)(P均<0.05);析因分析结果显示,PRF组、OAM组存在交互作用,OAM与PRF混合使用效果优于分别单独使用;愈合期PRF+OAM组拔牙创CT差值(35.33±2.08)均高于PRF组(33.33±3.51)、OAM组(-80.67±2.08)和对照组(-153.33±2.88)(P<0.05);术后4、12周PRF+OAM组拔牙窝新生骨排列规则度及成骨情况明显优于PRF组、OAM组和对照组(P<0.05).结论 PRF+ OAM应用于拔牙位点保存中可减缓牙槽嵴吸收,促进组织修复,其效果明显优于单独使用PRF和OAM.

  18. Expression and significance of OPG and RANKL protein in repairing alveolar bone defect with bone marrow stem cells(BMSCs) compounded with platelet-rich fibrin(PRF)%BMSCs复合PRF修复牙槽骨缺损中OPG和RANKL的表达及意义

    Institute of Scientific and Technical Information of China (English)

    张文丽; 陈诚; 李淑慧; 李一鸣; 周晶; 吴佩玲

    2015-01-01

    目的:探讨骨髓间充质干细胞(BMSCs)复合富血小板纤维蛋白(PRF)修复牙槽骨缺损中骨保护素(OPG)和核因子B受体活化因子配体(RANKL)的表达及意义。方法:取健康雄性2月龄新西兰兔36只,随机分为A、B、C、D 4组,均在全麻下微创拔除下颌左侧中切牙。 A组植入BMSCs与PRF复合物,B组植入PRF,C组植入BMSCs,D组为空白对照组。按术后4,8,12周3个时间点(每个时间点9只)处死动物并立即于骨缺损部位取材,免疫组织化学方法检测OPG和RANKL的表达。结果:4,8,12周时A组、B组、C组OPG的表达高于D组(P<0.05);4,8,12周时A组、B组、C组RANKL的表达高于D组(P<0.05);析因分析显示自体BMSCs复合PRF修复牙槽骨缺损中OPG,RANKL的表达高于单独使用BMSCs或PRF(P<0.05)。结论:自体BMSCs复合PRF修复牙槽骨缺损会增加OPG,RANKL的表达,有助于牙槽骨改建。%Objective:To observe expression and significance of osteoprotegerin(OPG) and receptor activator of NF kappa B ligand(RANKL) protein in alveolar bone repair with bone marrow stem cells(BMSCs) compounded with platelet-rich fibrin(PRF) in rabbits. Method:Thirty-six male 2 months rabbits were divided into four groups(A,B,C,D) randomly. A group:BMSCs/PRF composite;B group:PRF;C group:BMSCs;D group:blank control.They were minimally invasive re-moval mandible incisor under general anesthesia on the left side.The rabbits were sacarificed at 3 time point(4,8,12 week) with nine sacarificed per time postoperatively and then the jaws were taken out immediately. Immumohistochemistry was performed at each of 3 time points respectively to detect the expression of OPG and RANKL. Result:OPG protein expres-sion in group A,B,C was significantly higher than blank control at 4,8,12 week (P <0.05) and expression of RANKL in group A,B,C was significantly higher than blank control at 4,8,12 week (P<0.05);the

  19. Platelet-rich plasma for osteoarthritis treatment

    Directory of Open Access Journals (Sweden)

    Eduardo Knop

    2016-04-01

    Full Text Available ABSTRACT We conducted a comprehensive and systematic search of the literature on the use of platelet-rich plasma (PRP in the treatment of osteoarthritis, using the Medline, Lilacs, Cochrane and SciELO databases, from May 2012 to October 2013. A total of 23 studies were selected, with nine being controlled trials and, of these, seven randomized, which included 725 patients. In this series, the group receiving PRP showed improvement in pain and joint function compared to placebo and hyaluronic acid. The response lasted up to two years and was better in milder cases. However it was found that there is no standardization in the PRP production method, neither in the number, timing, and volume of applications. Furthermore, the populations studied were not clearly described in many studies. Thus, these results should be analyzed with caution, and further studies with more standardized methods would be necessary for a more consistent conclusion about the PRP role in osteoarthritis.

  20. Formulation and Storage of Platelet-Rich Plasma Homemade Product

    Science.gov (United States)

    Giraudo, Laurent; Veran, Julie; Magalon, Jeremy; Coudreuse, Jean-Marie; Magalon, Guy; Dubois, Christophe; Serratrice, Nicolas; Dignat-George, Françoise; Sabatier, Florence

    2012-01-01

    Abstract The platelet-rich plasma (PRP) is an autologous biotherapy based on platelet-healing properties. Here, we developed a simple and reproducible PRP purification protocol based on two successive centrifugations. We evaluated different centrifugation speeds and time-storage durations on the platelet quantity and quality. Sterility and stability of our PRP homemade product were also performed. We prepared PRP from 54 healthy volunteers. We tested activation state, reactivity, and stability of platelets by flow cytometry using basal and adenosine diphosphate (ADP)-induced P-selectin expression markers; growth factor release after platelet activation by an enzyme-linked immunosorbent assay (ELISA); platelet aggregation capacity by aggregrometry assays; clot formation and retraction by thromboelastography; and platelet morphology by ultrastructural analysis. About 130 and 250 g successive speed centrifugations further concentrated platelets while preserving their bioactivity during 6 h (after that, platelet functions were significantly altered). In these conditions, we obtained a highly concentrated pure PRP product (with a low leukocyte count) suitable to study platelet properties. To avoid the loss of efficacy, we recommend injecting PRP under 3 h after preparation. PMID:23516671

  1. Clinical efficacy of autologous fat grafting with platelet-rich plasma for facial rejuvenation%自体脂肪颗粒与富血小板血浆联合应用于面部年轻化的临床效果

    Institute of Scientific and Technical Information of China (English)

    齐向东; 周婕

    2016-01-01

    目的 探讨自体脂肪颗粒与富血小板血浆(PRP)联合应用于面部年轻化的临床效果.方法 随机选择30例自觉面部出现松垂或局部凹陷导致衰老容貌,要求进行自体脂肪填充美容就医者.填充术前及填充术后3个月随访时进行标准照相及三维扫描,美容就医者通过视觉模拟量表(VAS)对填充效果进行评分.医师通过填充术前和填充术后的影像资料,对美容就医者临床效果进行评价,并对美学结果及满意度分析,统计医师与美容就医者的评价差异.结果 医师满意度VAS评分为(7.9±1.0)分,美容就医者满意度VAS评分平为(8.0±1.2)分,两者差异无统计学意义(P>0.05).美容就医者评分小于7分的主要原因为面部凹陷改善未达到美容就医者要求,以及脂肪颗粒吸收较多导致填充效果不明显.有4例美容就医者填充术后出现明显的淤血及肿胀,均在7d内消失,没有美容就医者反映有明显不适.结论 PRP技术有效提高了移植脂肪存活率,以及医师和美容就医者的满意度.%Objective This single-center prospective,case-controlled study was carried out to investigate clinical effects on patients with aging face who underwent facial autologous fat grafting with platelet-rich plasma (PRP).Methods Thirty patients with facial sagging or partial depression and requiring autologous fat grafting were randomly selected for the study.Photograph and 3D scanning were taken before and 3-month after the operation.Patient and physician satisfaction was rated using Visual Analogue Scale (VAS) ranging from 1 (most unsatisfactory) to 10 (most satisfactory)3 months after the operation.Statistical difference between the patient and physician satisfactory scores was analyzed.Results The average physician VAS score was 7.9 ± 1.0 while the average patient VAS score was 8.0±1.2.The scores between two groups had no significant difference (P> 0.05).Four patients developed bruising and swelling

  2. Plasma rico en plaquetas Platelet -rich plasma

    Directory of Open Access Journals (Sweden)

    J. González Lagunas

    2006-04-01

    Full Text Available El Plasma Rico en Plaquetas es una suspensión concentrada de la sangre centrifugada que contiene elevadas concentraciones de trombocitos. Durante los últimos años, este producto ha aparecido de forma repetida en publicaciones científicas y en medios de comunicación generales como un producto que por sus características induce la curación y regeneración de los tejidos. La premisa de su uso es que las elevadas concentraciones de plaquetas en el PRP, liberan cantidades significativas de factores de crecimiento. En este artículo se van a recoger las evidencias científicas que se han presentado en la literatura médica con respecto al PRP y a la curación ósea, así como las diferentes aplicaciones clínicas que se han sugerido.Platelet-rich plasma is a by-product of centrifuged whole blood that contains high levels of thrombocytes. In the last decade, scientific and media interest has been generated by this product that apparently has the capacity of inducing and promoting tissue healing and regeneration. The premise of its use is that the large number of platelets in PRP release significant amounts of growth factors. In this paper, a critical review of the medical literature regarding PRP and bone healing will be presented. Also, the suggested clinical applications of the product will be addressed.

  3. A General Aspect of Platelet Rich Plasma

    Directory of Open Access Journals (Sweden)

    Onur ORAL

    2015-08-01

    Full Text Available The aim of this scientific paper is to introduce Platelet Rich Plasma (PRP cure method by people who never heard about it. People can hurt their selves, thus they can have damaged tissue; for instance broken bone, a scar or a wounded area. Furthe rmore damaged tissue can be a cartilage tissue, which takes very long time to heal. Platelets, those exist in the veins as thrombus, come up to repair those damaged tissues. However, platelets would be insufficient to cure damaged area in a short time. At this point PRP cure method give a hand to the healing process. By centrifuging people’s own blood via special kits, platelets can be separated from blood cells as plasma. That plasma’s platelet density is 3 - 5 times greater than that blood’s platelet densit y. Afterwards PRP method is implemented by injection of plasma to the damaged area or tissue. After implementation of 2 - 4 sessions per week, damaged tissue can be regenerated. It is fast healing method because densified platelet plasma is used; and it is s afe because that plasma is obtained from people’s own blood. PRP can be implemented on many areas; for instance on dentistry, sports medicine, different kind of surgeries such as plastic, vascular or orthopedic and so on. When soccer players brake their le gs, their sports life come to the end, but what if their broken legs was healed better and faster than general healing process? To sum up, PRP is very safe and the future of healing process.

  4. INTRALESIONAL PLATELET RICH PLASMA vs INTRALESIONAL TRIAMCINOLONE IN THE TREATMENT OF ALOPECIA AREATA: A COMPARATIVE STUDY

    Directory of Open Access Journals (Sweden)

    Shumez H, Prasad PVS, Kaviarasan PK, Deepika R

    2015-01-01

    Full Text Available Background: Alopecia areata (AA is a chronic non-scarring alopecia that involves the scalp and/or body, and is characterized by patchy areas of hair loss without any signs of clinical inflammation. Various therapies have been proposed for their treatment.But none have been shown to alter the course of the disease. Platelet Rich Plasma (PRP is a volume of autologous plasma that has a high platelet concentration. Growth factors released from platelets may act on stem cells in the bulge area of the follicles, stimulating the development of new follicles and promoting neovascularization. Aim: To evaluate and compare the efficacy of intralesional injection of autologous platelet rich plasma with intralesional injection of triamcinolone acetonide (10mg/ml in the treatment of alopecia areata. Methodology: 74 patients with alopecia areata were allocated into 2 groups and treated with triamcinolone and PRP injections. Treatment outcome was measured by taking into account extent and density of regrowth of hair and was expressed as a percentage of overall growth. Results: Forty eight patients were treated with triamcinolone injections and 26 patients were treated with PRP injections. Patients treated with PRP had an earlier response at the end of 6weeks than patients treated with triamcinolone. However, this difference was statistically insignificant. The overall improvement at the end of 9 weeks was 100% for all patients in both groups. Conclusion: PRP is a safe, simple, biocompatible and effective procedure for the treatment of alopecia areata with efficacy comparable with triamcinolone.

  5. Efficacy of platelet-rich plasma in treatment of androgenic alopecia

    Directory of Open Access Journals (Sweden)

    Parul Singhal

    2015-01-01

    Full Text Available Background: Platelet-rich plasma (PRP has shown remarkable beneficial effects without any major adverse reactions in the treatment of androgenic alopecia. The growth factors in activated autologous PRP induce the proliferation of dermal papilla cells. Objectives: The objective was to investigate the clinical efficacy of PRP in treatment of androgenic alopecia. Materials and Methods: Ten patients were given autologous PRP injections on the affected area of alopecia over a period of 3 months at interval of 2-3 weeks and results were assessed. Results: Three months after the treatment, the patients presented clinical improvement in the hair counts, hair thickness, hair root strength, and overall alopecia. Conclusion: PRP appears to be a cheap, effective, and promising therapy for androgenic alopecia with no major adverse effects.

  6. Evaluation of the Effects of Platelet-Rich Plasma (PRP) Therapy Involved in the Healing of Sports-Related Soft Tissue Injuries

    OpenAIRE

    Middleton, Kellie K.; Barro, Victor; Muller, Bart; Terada, Satosha; Fu, Freddie H.

    2012-01-01

    Musculoskeletal injuries are the most common cause of severe long-term pain and physical disability, and affect hundreds of millions of people around the world. One of the most popular methods used to biologically enhance healing in the fields of orthopaedic surgery and sports medicine includes the use of autologous blood products, namely, platelet rich plasma (PRP). PRP is an autologous concentration of human platelets to supra-physiologic levels. At baseline levels, platelets function as a ...

  7. Use of autologous human mesenchymal stromal cell/fibrin clot constructs in upper limb non-unions: long-term assessment.

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    Stefano Giannotti

    Full Text Available BACKGROUND: Tissue engineering appears to be an attractive alternative to the traditional approach in the treatment of fracture non-unions. Mesenchymal stromal cells (MSCs are considered an appealing cell source for clinical intervention. However, ex vivo cell expansion and differentiation towards the osteogenic lineage, together with the design of a suitable scaffold have yet to be optimized. Major concerns exist about the safety of MSC-based therapies, including possible abnormal overgrowth and potential cancer evolution. AIMS: We examined the long-term efficacy and safety of ex vivo expanded bone marrow MSCs, embedded in autologous fibrin clots, for the healing of atrophic pseudarthrosis of the upper limb. Our research work relied on three main issues: use of an entirely autologous context (cells, serum for ex vivo cell culture, scaffold components, reduced ex vivo cell expansion, and short-term MSC osteoinduction before implantation. METHODS AND FINDINGS: Bone marrow MSCs isolated from 8 patients were expanded ex vivo until passage 1 and short-term osteo-differentiated in autologous-based culture conditions. Tissue-engineered constructs designed to embed MSCs in autologous fibrin clots were locally implanted with bone grafts, calibrating their number on the extension of bone damage. Radiographic healing was evaluated with short- and long-term follow-ups (range averages: 6.7 and 76.0 months, respectively. All patients recovered limb function, with no evidence of tissue overgrowth or tumor formation. CONCLUSIONS: Our study indicates that highly autologous treatment can be effective and safe in the long-term healing of bone non-unions. This tissue engineering approach resulted in successful clinical and functional outcomes for all patients.

  8. Treatment of Hypertrophic Scar in Human with Autologous Transplantation of Cultured Keratinocytes and Fibroblasts along with Fibrin Glue

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    Ehsan Taghiabadi

    2015-04-01

    Full Text Available Objective: Hypertrophic scar involves excessive amounts of collagen in dermal layer and may be painful. Nowadays, we can’t be sure about effectiveness of procedure for hypertrophic scar management. The application of stem cells with natural scaffold has been the best option for treatment of burn wounds and skin defect, in recent decades. Fibrin glue (FG was among the first of the natural biomaterials applied to enhance skin deformity in burn patients. This study aimed to identify an efficient, minimally invasive and economical transplantation procedure using novel FG from human cord blood for treatment of hypertrophic scar and regulation collagen synthesis. Materials and Methods: In this case series study, eight patients were selected with hypertrophic scar due to full-thickness burns. Human keratinocytes and fibroblasts derived from adult skin donors were isolated and cultured. They were tested for the expression of cytokeratin 14 and vimentin using immunocytochemistry. FG was prepared from pooled cord blood. Hypertrophic scars were extensively excised then grafted by simply placing the sheet of FG containing autologous fibroblast and keratinocytes. Histological analyses were performed using Hematoxylin and eosin (H&E and Masson’s Trichrome (MT staining of the biopsies after 8 weeks. Results: Cultured keratinocytes showed a high level of cytokeratin 14 expression and also fibroblasts showed a high level of vimentin. Histological analyses of skin biopsies after 8 weeks of transplantation revealed re-epithelialization with reduction of hypertrophic scars in 2 patients. Conclusion: These results suggest may be the use of FG from cord blood, which is not more efficient than previous biological transporters and increasing hypertrophic scar relapse, but could lead to decrease pain rate.

  9. Platelet-Rich Plasma for Frozen Shoulder: A Case Report

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    Hamidreza Aslani

    2016-01-01

    Full Text Available Frozen shoulder is a glenohumeral joint disorder that perturbs movement because of adhesion and the existence of fibrosis in the shoulder capsule. Platelet-rich plasma can produce collagen and growth factors, which increases stem cells and consequently enhances the healing. To date, there is no evidence regarding the effectiveness of platelet-rich plasma in frozen shoulder. A 45-year-old man with shoulder adhesive capsulitis volunteered for this treatment. He underwent two consecutive platelet-rich plasma injections at the seventh and eighth month after initiation of symptoms. We measured pain, function, and ROM by the visual analogue scale (VAS, and scores from the Disabilities of the Arm, Shoulder and Hand (DASH questionnaire and goniometer; respectively. After the first injection, the patient reported 60% improvement regarding diurnal shoulder pain, and no night pains. Also, two-fold improvement for ROM and more than 70% improvement for function were reported. This study suggests the use of platelet-rich plasma in frozen shoulder to be tested in randomized trials.

  10. Platelet-rich plasma regenerative medicine sports medicine, orthopedic, and recovery of musculoskeletal injuries

    CERN Document Server

    Santana, Maria; Belangero, William; Luzo, Angela

    2014-01-01

    Platelet-Rich Plasma (PRP) has gained tremendous popularity in recent years as a treatment option for specialties including Orthopedics, Dentistry, Sports Medicine, Otorhinolaryngology, Neurosurgery, Ophthalmology, Urology, Vascular, Cardiothoracic and Maxillofacial Surgery, and Veterinarian Medicine. Nowadays, PRP and Stem Cell Science have added an exciting dimension to tissue repair. This book begins by giving the reader a broad overview of current progress as well as a discussion of the technical aspects of preparation and therapeutic use of autologous PRP. It is followed by a review of platelet structure, function and major growth factors in PRP (PDGF and TGFβ).The third chapter outlines the basic principles of biochemical cellular metabolism that increases the efficacy of PRP. Analogous to the preparation of soil for a garden, restoring cellular health should be the first consideration in Regenerative Medicine. Standardization of PRP preparation to clinical use still remains a challenging prospect. In ...

  11. Treatment of a non-healing diabetic foot ulcer with platelet-rich plasma.

    Science.gov (United States)

    Suresh, Deepak H; Suryanarayan, Shwetha; Sarvajnamurthy, Sacchidanand; Puvvadi, Srikanth

    2014-01-01

    Lower extremity ulcers and amputations are an increasing problem among individuals with diabetes. Among diabetes mellitus-related complications, foot ulceration is the most common, affecting approximately 15% of diabetic patients during their lifetime. The pathogenesis of diabetic ulcer is peripheral sensory neuropathy, calluses, oedema and peripheral vascular disease. Diabetic ulcer is managed by adequate control of infections and blood sugar levels, surgical debridement with various dressings and off loading of the foot from pressure. In spite of these standard measures, some recalcitrant non-healing ulcers need additional growth factors for healing. Autologous platelet-rich plasma is easy and cost-effective method in treating diabetic ulcers as it provides necessary growth factors which enhance healing.

  12. Applications of platelet-rich plasma in dermatology: A critical appraisal of the literature.

    Science.gov (United States)

    Lynch, M D; Bashir, S

    2016-01-01

    Platelet-rich plasma (PRP) is an autologous blood-derived product enriched in platelets, growth factors, chemokines and cytokines. Initial applications were predominantly in musculoskeletal and maxillofacial fields, however in recent years, it has been used for a range of dermatological indications including wound healing, fat grafting, alopecia, scar revision and dermal volume augmentation. Here, we critically appraise the literature relating to the usage of PRP within Dermatology. We have evaluated in vitro data, preclinical animal studies and human trials. We conclude that, whilst the literature may be consistent with a modest benefit for specific indications, there is not sufficient evidence supporting the efficacy of PRP to justify a role in routine dermatological practice at the present time. However, since PRP is generally well tolerated with few reported complications, further study may be justified in the context of organized trials.

  13. Role of platelet-rich plasma in ischemic heart disease: An update on the latest evidence

    Institute of Scientific and Technical Information of China (English)

    Eleftherios; Spartalis; Periklis; Tomos; Demetrios; Moris; Antonios; Athanasiou; Charalampos; Markakis; Michael; D; Spartalis; Theodore; Troupis; Dimitrios; Dimitroulis; Despina; Perrea

    2015-01-01

    Myocardial infarction is the most common cause of congestive heart failure. Novel strategies such as directly reprogramming cardiac fibroblasts into cardiomyocytes are an exciting area of investigation for repair of injured myocardial tissue. The ultimate goal is to rebuild functional myocardium by transplanting exogenous stem cells or by activating native stem cells to induce endogenous repair. Cell-based myocardial restoration, however, has not penetrated broad clinical practice yet. Plateletrich plasma, an autologous fractionation of whole blood containing high concentrations of growth factors, has been shown to safely and effectively enhance healing and angiogenesis primarily by reparative cell signaling. In this review, we collected all recent advances in novel therapies as well as experimental evidence demonstrating the role of platelet-rich plasma in ischemic heart disease, focusing on aspects that might be important for future successful clinical application.

  14. No effect of platelet-rich plasma with frozen or processed bone allograft around noncemented implants

    DEFF Research Database (Denmark)

    Jensen, T B; Rahbek, O; Overgaard, S;

    2005-01-01

    We compared processed morselized bone allograft with fresh-frozen bone graft around noncemented titanium implants. Also, the influence of platelet-rich plasma (PRP) in combination with bone allograft was evaluated. Analysis was based on implant fixation and histomorphometry. PRP was prepared...... by isolating the buffy coat from autologous blood samples. Bone allograft was used fresh-frozen or processed by defatting, freeze drying, and irradiation. Cylindrical hydroxyapatite-coated titanium implants were inserted bilaterally in the femoral condyles of eight dogs. Each implant was surrounded by a 2.5-mm...... concentric gap, which was filled randomly according to the four treatment groups--group 1: fresh-frozen bone allograft; group 2: processed bone allograft; group 3: fresh-frozen bone allograft + PRP; group 4: processed bone allograft + PRP. Histological and mechanical evaluation demonstrated no influence...

  15. Treatment of a non-healing diabetic foot ulcer with platelet-rich plasma

    Directory of Open Access Journals (Sweden)

    Deepak H Suresh

    2014-01-01

    Full Text Available Lower extremity ulcers and amputations are an increasing problem among individuals with diabetes. Among diabetes mellitus-related complications, foot ulceration is the most common, affecting approximately 15% of diabetic patients during their lifetime. The pathogenesis of diabetic ulcer is peripheral sensory neuropathy, calluses, oedema and peripheral vascular disease. Diabetic ulcer is managed by adequate control of infections and blood sugar levels, surgical debridement with various dressings and off loading of the foot from pressure. In spite of these standard measures, some recalcitrant non-healing ulcers need additional growth factors for healing. Autologous platelet-rich plasma is easy and cost-effective method in treating diabetic ulcers as it provides necessary growth factors which enhance healing.

  16. Platelet-rich plasma (PRP) applied during total knee arthroplasty☆

    Science.gov (United States)

    Guerreiro, João Paulo Fernandes; Danieli, Marcus Vinicius; Queiroz, Alexandre Oliveira; Deffune, Elenice; Ferreira, Rosana Rossi

    2015-01-01

    Objective To evaluate the efficacy of platelet-rich plasma regarding healing, pain and hemostasis after total knee arthroplasty, by means of a blinded randomized controlled and blinded clinical study. Methods Forty patients who were going to undergo implantation of a total knee prosthesis were selected and randomized. In 20 of these patients, platelet-rich plasma was applied before the joint capsule was closed. The hemoglobin (mg/dL) and hematocrit (%) levels were assayed before the operation and 24 and 48 h afterwards. The Womac questionnaire and a verbal pain scale were applied and knee range of motion measurements were made up to the second postoperative month. The statistical analysis compared the results with the aim of determining whether there were any differences between the groups at each of the evaluation times. Results The hemoglobin (mg/dL) and hematocrit (%) measurements made before the operation and 24 and 48 h afterwards did not show any significant differences between the groups (p > 0.05). The Womac questionnaire and the range of motion measured before the operation and up to the first two months also did not show any statistical differences between the groups (p > 0.05). The pain evaluation using the verbal scale showed that there was an advantage for the group that received platelet-rich plasma, 24 h, 48 h, one week, three weeks and two months after the operation (p < 0.05). Conclusions In the manner in which the platelet-rich plasma was used, it was not shown to be effective for reducing bleeding or improving knee function after arthroplasty, in comparison with the controls. There was an advantage on the postoperative verbal pain scale. PMID:26229915

  17. Platelet-rich plasma (PRP applied during total knee arthroplasty

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    João Paulo Fernandes Guerreiro

    2015-04-01

    Full Text Available OBJECTIVE: To evaluate the efficacy of platelet-rich plasma regarding healing, pain and hemostasis after total knee arthroplasty, by means of a blinded randomized controlled and blinded clinical study.METHODS: Forty patients who were going to undergo implantation of a total knee prosthesis were selected and randomized. In 20 of these patients, platelet-rich plasma was applied before the joint capsule was closed. The hemoglobin (mg/dL and hematocrit (% levels were assayed before the operation and 24 and 48 h afterwards. The Womac questionnaire and a verbal pain scale were applied and knee range of motion measurements were made up to the second postoperative month. The statistical analysis compared the results with the aim of determining whether there were any differences between the groups at each of the evaluation times.RESULTS: The hemoglobin (mg/dL and hematocrit (% measurements made before the operation and 24 and 48 h afterwards did not show any significant differences between the groups (p > 0.05. The Womac questionnaire and the range of motion measured before the operation and up to the first two months also did not show any statistical differences between the groups (p > 0.05. The pain evaluation using the verbal scale showed that there was an advantage for the group that received platelet-rich plasma, 24 h, 48 h, one week, three weeks and two months after the operation (p < 0.05.CONCLUSIONS: In the manner in which the platelet-rich plasma was used, it was not shown to be effective for reducing bleeding or improving knee function after arthroplasty, in comparison with the controls. There was an advantage on the postoperative verbal pain scale.

  18. Concentration of platelets and growth factors in platelet-rich plasma from Goettingen minipigs

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    Jungbluth, Pascal

    2014-11-01

    Full Text Available In minipigs little is known about the concentration of growth factors in plasma, despite their major role in several patho-physiological processes such as healing of fractures. This prompted us to study the concentration of platelets and selected growth factors in plasma and platelet-rich plasma (PRP preparation of sixteen Goettingen minipigs. Platelet concentrations increased significantly in PRP in comparison to native blood plasma. Generally, significant increase in the concentration of all growth factors tested was observed in the PRP in comparison to the corresponding plasma or serum. Five of the plasma samples examined contained detectable levels of bone morphogenic protein 2 (BMP-2 whereas eleven of the plasma or serum samples contained minimal amounts of vascular endothelial growth factor (VEGF and platelet-derived growth factor (PDGF-bb respectively. On the other hand variable concentrations of bone morphogenic protein 7 (BMP-7 and transforming growth factor β1 (TGF-β1 were measured in all plasma samples. In contrast, all PRP samples contained significantly increased amounts of growth factors. The level of BMP-2, BMP-7, TGF-β1, VEGF and PDGF-bb increased by 17.6, 1.5, 7.1, 7.2 and 103.3 fold, in comparison to the corresponding non-enriched preparations. Moreover significant positive correlations were found between platelet count and the concentrations of BMP-2 (r=0.62, p<0.001, TGF-β1 (r=0.85, p<0.001, VEGF (r=0.46, p<0.01 and PDGF-bb (r=0.9, p<0.001. Our results demonstrate that selected growth factors are present in the platelet-rich plasma of minipigs which might thus serve as a source of autologous growth factors.

  19. Platelet-rich plasma as treatment for persistent ocular epithelial defects.

    Science.gov (United States)

    Ronci, Corrado; Ferraro, Angelo Salvatore; Lanti, Alessandro; Missiroli, Filippo; Sinopoli, Silvia; Del Proposto, Gianpaolo; Cipriani, Chiara; De Felici, Cecilia; Ricci, Federico; Ciotti, Marco; Cudillo, Laura; Arcese, William; Adorno, Gaspare

    2015-06-01

    Platelet- rich plasma (PRP) exhibits regenerative proprieties in wound healing but the biochemical mechanisms are unclear. In this study, autologous PRP with a mean value of 338 × 10(3) platelets/µL was used to treat corneal lesions of different aetiology, while homologous PRP with 1 × 10(6) platelets/µL was used to treat cornel lesions induced by a graft versus host disease. The impact of platelet count on the levels of PDGF AA and BB, VEGF, and EGF in the two PRPs was evaluated after a cycle of freezing/thawing. Treated corneal lesions healed or improved. The levels of PDGF AA and BB, VEGF, and EGF in the autologous PRP raised from 296 ± 61; 201.8 ± 24; 53 ± 14 and 8.9 ± 2 to 1017 ± 253; 924.7 ± 222; 101 ± 46.5 and 174 ± 15.5 pg/mL, while in the homologous PRP were 3.4, 4.5, 3.2 and 2 folds higher, respectively. High level of platelet counts seems not required to treat corneal lesions.

  20. Platelet-rich plasma as treatment for persistent ocular epithelial defects.

    Science.gov (United States)

    Ronci, Corrado; Ferraro, Angelo Salvatore; Lanti, Alessandro; Missiroli, Filippo; Sinopoli, Silvia; Del Proposto, Gianpaolo; Cipriani, Chiara; De Felici, Cecilia; Ricci, Federico; Ciotti, Marco; Cudillo, Laura; Arcese, William; Adorno, Gaspare

    2015-06-01

    Platelet- rich plasma (PRP) exhibits regenerative proprieties in wound healing but the biochemical mechanisms are unclear. In this study, autologous PRP with a mean value of 338 × 10(3) platelets/µL was used to treat corneal lesions of different aetiology, while homologous PRP with 1 × 10(6) platelets/µL was used to treat cornel lesions induced by a graft versus host disease. The impact of platelet count on the levels of PDGF AA and BB, VEGF, and EGF in the two PRPs was evaluated after a cycle of freezing/thawing. Treated corneal lesions healed or improved. The levels of PDGF AA and BB, VEGF, and EGF in the autologous PRP raised from 296 ± 61; 201.8 ± 24; 53 ± 14 and 8.9 ± 2 to 1017 ± 253; 924.7 ± 222; 101 ± 46.5 and 174 ± 15.5 pg/mL, while in the homologous PRP were 3.4, 4.5, 3.2 and 2 folds higher, respectively. High level of platelet counts seems not required to treat corneal lesions. PMID:25728718

  1. A standardized research protocol for platelet- rich plasma (PRP preparation in rats

    Directory of Open Access Journals (Sweden)

    Michel Reis Messora

    2011-07-01

    Full Text Available Introduction: The urgent need for studies using standardized protocols to evaluate the real biological effects of PRP has been emphasized by several authors. Objective: The purpose of this study was to standardize a methodology for autologous Platelet-Rich Plasma (PRP preparation in rats. Material and methods: Twenty-four, 5 to 6-month-old, male rats, weighing 450 to 500 g were used. After general anesthesia, 3.15 ml of blood was collected from each animal, via cannulation of the jugular vein. A standardized technique of double centrifugation was used to prepare PRP. PRP samples and peripheral blood platelets were then manually counted using a Neubauer chamber. Student’s t-test was used to compare the differences between the number of platelets in peripheral blood and PRP samples (p < 0.05. In addition, PRP and peripheral blood smears were stained to see platelets’ morphology. Results: All surgical procedures were well tolerated by the animals and they were healthy during the entire experimental period. PRP samples showed higher significantly platelet concentrations than peripheral blood samples (2,677,583 and 683,680 respectively. Conclusion: Within the limits of this study, it can be concluded that the method used produced autologous PRP with appropriated platelet quantity and quality, in rats.

  2. 富血小板纤维蛋白的研究进展%Recent advance in research of platelet-rich plasma

    Institute of Scientific and Technical Information of China (English)

    戴勇中; 叶平

    2011-01-01

    @@ 口腔种植的骨增量技术扩大了种植适应证,但手术复杂、术后反应重,且骨移植材料和自体组织的整合仍然存在不少问题.由于血小板富有多种生长因子,血小板浓缩物被用于改善术后反应、促进骨和软组织愈合.研究较多的血小板浓缩物是富血小板血浆(platelet-rich plasma,PRP).近年来富血小板纤维蛋白(platelet-rich fibrin,PRF)的研究和应用成为热点.

  3. Alternative method to treat oroantral communication and fistula with autogenous bone graft and platelet rich firbin

    Science.gov (United States)

    Niedzielska, Iwona; Borgiel-Marek, Halina; Różanowski, Bartosz

    2016-01-01

    Background Removing a tooth from the jaw results in the occurrence of oroantral communication in beneficial anatomic conditions or in the case of a iatrogenic effect. Popularized treatments of the oroantral communication have numerous faults. Large bone defect eliminates the chance to introduce an implant. Purpose of this work was assessment of the usefulness of autogenous bone graft and PRF in normal bone regeneration in the site of oroantral communication. Material and Methods Bone regeneration in the site of oroantral communication was assessed in 20 patients. Bone defects were supplemented autogenous bone graft from mental protuberance in 14 cases and from oblique line in 6 cases. The graft was covered with a PRF membrane. Results In the study group in all cases closure of the oroantral communication was observed. The average width of the alveolar was 13 mm and the average height was 12.5 mm. In 3 patients an average increase of alveolar height of 1.5 mm was observed. Conclusions This method may be the best option to prepare alveolar for new implant and prosthetic solutions. Key words:Oroantral communication, oroantral fistula, autogenous bone graft, bone regeneration, platelet rich fibrin. PMID:27475687

  4. Clinical application and Fuzzy evaluation of autologous platelet-rich plasma mixed with fat graft in facial tissue depression%自体富血小板血浆-脂肪颗粒填充面部凹陷的临床应用及Fuzzy评价

    Institute of Scientific and Technical Information of China (English)

    王昕; 陈小平; 林金德; 石春龙; 郑翔宇; 时梦竹

    2014-01-01

    目的 评价自体富血小板血浆-脂肪颗粒填充面部凹陷的临床疗效.方法 采用自体富血小板血浆加自体脂肪颗粒,对35例面部凹陷患者进行填充修复.3~6个月后,用Fuzzy评判法(模糊评判法)对面部凹陷部丰满度、注射次数、手术满意度进行评价.结果 术后患者面部获得了一个相对对称且自然的外观,填充修复效果稳定,未见明显脂肪吸收.医患双方均对手术效果满意.综合评判结果表明有效这一隶属度最高.结论 自体富血小板血浆应用于面部脂肪移植,有助于脂肪存活和减少脂肪吸收,填充矫治面部软组织缺损畸形效果确切可靠.%Objective To evaluate the clinical effect of the facial soft tissue defect treated with platelet-rich plasma (PRP) mixed with purified fat.Methods 35 patients with facial soft-tissue defects were treated by PRP mixed with centrifuged fat tissue.Using Fuzzy judgment method the clinical effectiveness of therapeutic outcomes were evaluated on fullness,injection of frequency and satisfaction during 3 to 6 months of follow-up.Results The therapeutic outcomes showed that the reconstructed face became a relative symmetrical and natural appearance with not evidently fat resorption and stable after operation.Both patients and surgeons were satisfied with the results.The score of effectiveness was highest in Fuzzy judgment.Conclusions The efficacy of PRP is significant in enhancing fat graft,reducing fat necrosis and resorption.The fat tissue mixed with PRP is a reliable reconstruction option for the facial soft tissue defect.

  5. Osteogenic potential of human umbilical cord-derived mesenchymal stromal cells cultured with umbilical cord blood-derived fibrin: a preliminary study.

    Science.gov (United States)

    Baba, Kyoko; Yamazaki, Yasuharu; Ishiguro, Masashi; Kumazawa, Kenichi; Aoyagi, Kazuya; Ikemoto, Shigehiro; Takeda, Akira; Uchinuma, Eiju

    2013-12-01

    This study examined the potential for osteogenesis via regenerative medicine using autologous tissues (umbilical cord (UC) and umbilical cord blood (UCB)) in nude mice. The study was designed to provide the three elements required for regenerative medicine (cell, scaffold, and growth factor) and autoserum for culture by means of autologous tissues. Mesenchymal stromal cells were obtained from UC (UC-MSCs). Fibrin, platelet-rich-plasma, and autoserum were obtained from UCB as scaffold, growth factor and serum for culture respectively. UC-MSCs were obtained from Wharton jelly and cultured with UCB-derived fibrin (UCB-fibrin) for 3-4 weeks to induce their differentiation into osteoblasts. They were implanted subcutaneously into the dorsum of male nude mice for 6 weeks prior to undergoing assessment. The assessments performed were haematoxylin and eosin, and alizarin red staining, immunohistochemical staining of human mitochondria, scanning electron microscopy, scanning electron microscopy with energy dispersive X-ray spectrometry and real-time reverse transcriptase-polymerase chain reaction to assess the expressions of osteoblast markers. Consequently, the differentiation of UC-MSCs into osteoblasts and the production of hydroxyapatite were verified. This study suggested the possible formation of bone tissue using biomedical materials obtained from UC and UCB.

  6. Platelet rich plasma. A new treatment tool for the rheumatologist?

    Science.gov (United States)

    De La Mata, José

    2013-01-01

    Platelet Rich Plasma (PRP) is a novel therapeutic tool that has revolutionized the world of sports medicine and trauma due to therapeutic success shown in the media. Subject to ongoing debate, the PRP is outlined along a spectrum of musculoskeletal therapies with many qualities that make it ideal for use in the rheumatology: effectiveness, safety, easy handling and low cost. Is PRP a product of marketing? Or, conversely, is an interesting tool to consider in the armamentarium of the rheumatologist. In the following review we will analyze in detail its principles, preparation, and management regimes. We will reflect on potential adverse effects and, finally, there will be a critical analysis of the scientific evidence that supports its potential use in the rheumatology clinic. PMID:22902984

  7. Platelet Rich Plasma: a short overview of certain bioactive components

    Directory of Open Access Journals (Sweden)

    Pavlovic Voja

    2016-08-01

    Full Text Available Platelet rich plasma (PRP represents a relatively new approach in regenerative medicine. It is obtained from patient’s own blood and contains different growth factors and other biomolecules necessary for wound healing. Since there are various protocols for PRP preparing, it usually results with PRP generation with different amounts of bioactive substances, which finally may modulate the intensity of wound healing. The reference data about potential effect of some PRP compounds on wound healing, in different tissues, are still controversial. This review summarizes recently known facts about physiological role of certain PRP components and guidance for further research. Also, this review discusses different procedure for PRP generation and potential effect of leukocytes on wound healing.

  8. Timing of Platelet Rich Plasma Injections During Antithrombotic Therapy.

    Science.gov (United States)

    Ramsook, Ryan Ravi; Danesh, Houman

    2016-01-01

    The use of platelet rich plasma (PRP) spans across many fields owing to its role in healing and as a natural alternative to surgery. PRP continues to grow however much of the literature is anecdotal or case report based and there is a lack of controlled trials to evaluate standards for PRP. The International Cellular Medical Society (ICMS) has developed guidelines to help with the safe advancement of PRP; however there remains a gap in literature concerning the timing of PRP injections in patients who are on antithrombotic therapy. The importance of an intact platelet surface membrane allows for the appropriate release of the healing bioproteins and growth factors granting PRP therapy its efficacy. This along with the proliferation of differentiated cells, enhancement of collagen synthesis, early angiogenesis and revascularization help promote the benefits of regeneration. The intrinsic and extrinsic pathways of the coagulation cascade are valuable in that disruption of this mechanism or prematurely activated platelets may result in limited efficacy. Anticoagulants and antiplatelet drugs are commonly used in patients who are candidates for PRP. As antithrombotic agents affect platelet stability, they will have an effect on PRP efficacy and must be discontinued at an appropriate time frame prior to injection therapy. Understanding the pharmacokinetics and platelet effects can help guide discussion on the proper timing of discontinuation and resumption of a particular antithrombotic agent. With future research, the establishment of clinical practice guidelines concerning PRP and antithrombotic therapy can help structure safe and efficacious means in which to promote healing and regeneration in a growing patient population. Platelet rich plasma, antithrombotic therapy, coagulation, platelet activation, regenerative medicine, growth factors. PMID:27676677

  9. Platelet rich plasma for treatment of nonhealing diabetic foot ulcers: a case report.

    Science.gov (United States)

    Mehrannia, Masoud; Vaezi, Mitra; Yousefshahi, Fardin; Rouhipour, Nahid

    2014-02-01

    Diabetic foot ulcers are one of the most important causes of lower limb amputations worldwide. The conventional treatments of diabetic foot ulcers are costly and often require patients to be hospitalized for long periods of time, thus representing a huge burden on any health care system. The use of autologous platelet-rich plasma (PRP), which is rich in multiple growth factors, may bear some similarities to the natural wound healing process. Nonetheless, few studies on human subjects have so far addressed the efficacy of PRP as a novel and minimally invasive treatment. Today, there is only 1 approved and available system to separate PRP from a patient's own blood in order to be used in diabetic ulcers. This system incorporates bovine thrombin for activation of PRP gel and may be applied by many healthcare providers without the need for extensive special training. In this report, a patient with extensive diabetic foot ulcers, non-responsive to other treatment modalities, was successfully treated by PRP.

  10. The influence of environmental variables on platelet concentration in horse platelet-rich plasma.

    Science.gov (United States)

    Rinnovati, Riccardo; Romagnoli, Noemi; Gentilini, Fabio; Lambertini, Carlotta; Spadari, Alessandro

    2016-01-01

    Platelet-rich plasma (PRP) commonly refers to blood products which contain a higher platelet (PLT) concentration as compared to normal plasma. Autologous PRP has been shown to be safe and effective in promoting the natural processes of soft tissue healing or reconstruction in humans and horses. Variability in PLT concentration has been observed in practice between PRP preparations from different patients or from the same individual under different conditions. A change in PLT concentration could modify PRP efficacy in routine applications. The aim of this study was to test the influence of environmental, individual and agonistic variables on the PLT concentration of PRP in horses. Six healthy Standardbred mares were exposed to six different variables with a one-week washout period between variables, and PRP was subsequently obtained from each horse. The variables were time of withdrawal during the day (morning/evening), hydration status (overhydration/dehydration) treatment with anti-inflammatory drugs and training periods on a treadmill. The platelet concentration was significantly higher in horses treated with a non-steroidal anti-inflammatory drug (P = 0.03). The leukocyte concentration increased 2-9 fold with respect to whole blood in the PRP which was obtained after exposure to all the variable considered. Environmental variation in platelet concentration should be taken into consideration during PRP preparation. PMID:27377748

  11. Platelet and growth factor concentrations in activated platelet-rich plasma: a comparison of seven commercial separation systems.

    Science.gov (United States)

    Kushida, Satoshi; Kakudo, Natsuko; Morimoto, Naoki; Hara, Tomoya; Ogawa, Takeshi; Mitsui, Toshihito; Kusumoto, Kenji

    2014-06-01

    Platelet-rich plasma (PRP) is blood plasma that has been enriched with platelets. It holds promise for clinical use in areas such as wound healing and regenerative medicine, including bone regeneration. This study characterized the composition of PRP produced by seven commercially available separation systems (JP200, GLO PRP, Magellan Autologous Platelet Separator System, KYOCERA Medical PRP Kit, SELPHYL, MyCells, and Dr. Shin's System THROMBO KIT) to evaluate the platelet, white blood cell, red blood cell, and growth factor concentrations, as well as platelet-derived growth factor-AB (PDGF-AB), transforming growth factor beta-1 (TGF-β1), and vascular endothelial growth factor (VEGF) concentrations. PRP prepared using the Magellan Autologous Platelet Separator System and the KYOCERA Medical PRP Kit contained the highest platelet concentrations. The mean PDGF-AB concentration of activated PRP was the highest from JP200, followed by the KYOCERA Medical PRP Kit, Magellan Autologous Platelet Separator System, MyCells, and GLO PRP. TGF-β1 and VEGF concentrations varied greatly among individual samples, and there was almost no significant difference among the different systems, unlike for PDGF. The SELPHYL system produced PRP with low concentrations of both platelets and growth factors. Commercial PRP separation systems vary widely, and familiarity with their individual advantages is important to extend their clinical application to a wide variety of conditions. PMID:24748436

  12. Effect of Topical Platelet-Rich Plasma on Burn Healing After Partial-Thickness Burn Injury.

    Science.gov (United States)

    Ozcelik, Umit; Ekici, Yahya; Bircan, Huseyin Yuce; Aydogan, Cem; Turkoglu, Suna; Ozen, Ozlem; Moray, Gokhan; Haberal, Mehmet

    2016-06-05

    BACKGROUND To investigate the effects of platelet-rich plasma on tissue maturation and burn healing in an experimental partial-thickness burn injury model. MATERIAL AND METHODS Thirty Wistar albino rats were divided into 3 groups of 10 rats each. Group 1 (platelet-rich plasma group) was exposed to burn injury and topical platelet-rich plasma was applied. Group 2 (control group) was exposed to burn injury only. Group 3 (blood donor group) was used as blood donors for platelet-rich plasma. The rats were killed on the seventh day after burn injury. Tissue hydroxyproline levels were measured and histopathologic changes were examined. RESULTS Hydroxyproline levels were significantly higher in the platelet-rich plasma group than in the control group (P=.03). Histopathologically, there was significantly less inflammatory cell infiltration (P=.005) and there were no statistically significant differences between groups in fibroblast development, collagen production, vessel proliferations, or epithelization. CONCLUSIONS Platelet-rich plasma seems to partially improve burn healing in this experimental burn injury model. As an initial conclusion, it appears that platelet-rich plasma can be used in humans, although further studies should be performed with this type of treatment.

  13. Effectiveness of Agents Like Platelet-rich Plasma, Oxidized Regenerated Cellulose and Microfibrillar Collagen in Hard-tissue Healing: Validation of t heir Comparative Effectiveness

    Directory of Open Access Journals (Sweden)

    Khalid Al Fouzan

    2015-07-01

    Full Text Available Autologous platelet-rich plasma (PRP can be a valid agent that is effective in inducing and accelerating bone healing for the treatment of periodontal defects and also to accelerate alveolar bone regeneration. Early healing appeared to be impaired by the presence of microfibrillar collagen (MFC and impeded by the presence of oxidized regenerated cellulose (ORC. In contrast, alkylene oxide copolymer (AOC did not inhibit bone healing and suggest that AOC may be a better bone hemo - static material for procedures where bony fusion is critical and immediate hemostasis required

  14. Bioactivity and stability of endogenous fibrogenic factors in platelet-rich fibrin

    DEFF Research Database (Denmark)

    Lundquist, Rasmus; Dziegiel, Morten H; Agren, Magnus S

    2008-01-01

    concentrate, recombinant human platelet-derived growth factor (rhPDGF) isoforms, and a homologous FS in cultured normal human dermal fibroblasts. Also, the release of selected endogenous growth factors from PRF and their stability against proteolytic degradation were studied. The proliferative effect of PRF...

  15. Management of Radicular Cyst Using Platelet-Rich Fibrin & Iliac Bone Graft - A Case Report.

    Science.gov (United States)

    Vidhale, Gaurav; Jain, Deepali; Jain, Sourabh; Godhane, Alkesh Vijayrao; Pawar, Ganesh R

    2015-06-01

    Radicular cyst is one of the most commonly occurring cyst in the oral cavity it is usually preceded by trauma or an infectious condition which is followed by enlargement. In recent times there are several treatment procedures that are being applied in order to improve the postoperative condition and to accelerate the process of healing and regeneration in the affected site. A 22-year-old patient reported to our OPD with the chief complaint of swelling on the left side of the face since 2-3 months, on investigating it was diagnosed as Radicular cyst which was initially treated by endodontic treatment of the involved tooth followed by enucleation of the cyst further an apicoectomy was done. Finally a PRF and iliac crest graft was placed for aesthetic rehabilitation with 21. PMID:26266233

  16. Use of photoactivated disinfection and platelet-rich fibrin in regenerative Endodontics

    OpenAIRE

    Dexton Antony Johns; Vasundara Yayathi Shivashankar; Shoba Krishnamma; Manu Johns

    2014-01-01

    Aim: Photoactivated disinfection has been used as an adjunct to conventional endodontic treatment. Its use in regenerative endodontics is not reported in literature. The aim of this case report was to describe a new proposal for pulp revascularization with disinfection of pulp canal space using a unique combination of a photosensitizer solution and low-power laser light. Materials and Methods: A 9-year-old boy came with the chief complaint of discolored upper central incisors (#8, #9). A ...

  17. Does Platelet-Rich Plasma Increase Tendon Metabolism?

    Science.gov (United States)

    de Vos, Robert-Jan

    2016-01-01

    Acute and overuse tendon disorders are frequently observed in the middle-aged active population. Tendon overuse injuries are currently designated as "tendinopathy". Histopathological studies have shown that chronic tendinopathy is frequently characterised by degenerative changes, such as decreased organisation of collagen, altered cell distribution and neovascularisation. In the recent years, scientific research and technology in the field of regenerative medicine has provided a new perspectives on managing chronic tendinopathy. An initiation of tissue healing can be attempted by local delivery of growth factors. Nowadays, platelet-rich plasma (PRP) is a commonly applied approach to achieve this. Platelet degranulation leads to a release of various growth factors and cytokines. There is a classification system to define the different forms of PRP. In the past decade, a number of studies have been published on the effects of PRP in different basic science studies. These studies suggest that PRP modulates some aspects of tendon metabolic activity. This is one of the reasons why PRP is increasingly used by many clinicians as treatment option for tendinopathy in daily clinical practice. There is, however, evidence from the literature that it does not lead to improved outcome on imaging findings and on patient-reported outcomes. This questions the role of PRP injections as regular treatment for tendinopathy. Moreover, it results in a broader discussion on the required effects that need to occur for tendon healing and symptom relieve. PMID:27535268

  18. Platelet rich plasma to facilitate wound healing following tooth extraction.

    Science.gov (United States)

    Rutkowski, James L; Johnson, David A; Radio, Nicholas M; Fennell, James W

    2010-01-01

    Following tooth removal bone formation normally takes 16 weeks and may result in less than adequate volume for the necessary reconstruction. Platelet rich plasma (PRP) has been promoted as an effective method for improving bone formation. Its use is often expensive, time consuming, or not clinically convenient for the patient and/or clinician. This study examines a simple method for obtaining a "Buffy Coat"-PRP (BC-PRP) and its effect on bone healing following the removal of bilateral mandibular 3rd molars. Subtraction digital radiography and CT scan analysis were used to track changes in radiographic density at PRP treated sites in comparison to ipsilateral non-PRP treated sites. PRP treated sites demonstrated early and significant increased radiographic density over baseline measurements following tooth removal. The greatest benefit of PRP is during the initial 2-week postoperative healing time period (P .05). For the entire time period (0-25 weeks) PRP treatment was significant (P .05) between the effect of PRP treatment and time. It required 6 weeks for control extraction sites to reach comparable bone density that PRP treated sites achieved at week 1. Postoperative pain, bleeding, and numbness were not significantly affected by BC-PRP application. Results suggest that this simple technique may be of value to clinicians performing oral surgery by facilitating bone regeneration following tooth extraction. PMID:20218866

  19. Treatment of Tendon Injuries of the Lower Limb with Growth Factors Associated with Autologous Fibrin Scaffold or Collagenous Scaffold.

    Science.gov (United States)

    Giannotti, Stefano; Dell'Osso, Giacomo; Bottai, Vanna; Ghilardi, Marco; Bugelli, Giulia; Lazzerini, Ilaria; Guido, Giulio

    2015-05-01

    Tendon injuries are an increasing problem in orthopedics as we are faced with a growing demand in sports and recreation and an aging population. Tendons have poor spontaneous regenerative capacity, and often, complete recovery after injury is not achieved. Once injured, tendons do not completely re-acquire the biological and biomechanical properties of normal tendons due to the formation of adhesions and scarring, and often these abnormalities in the arrangement and structure are risk factors for re-injury. These problems associated with the healing of tendon injuries are a challenge for clinicians and surgeons. This study examined 9 cases of subcutaneous injuries including quadriceps tendon (2 cases), patellar tendon (1 case), and Achilles tendon (6 cases), incomplete and complete, treated consecutively. The surgical technique has provided, as appropriate, the termino-terminal tenorraphy, techniques of plastics of rotation flap, reinsertion with suture anchors, and in one case tendon augmentation with cadaver tissue. In cases where we needed mechanical support to the suture, we used preloaded growth factors on porcine collagen scaffold; in cases where we needed only one biological support, we used fibrin scaffold. PMID:26055027

  20. Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel

    OpenAIRE

    Giraldo Carlos E; López Catalina; Álvarez María E; Samudio Ismael J; Prades Marta; Carmona Jorge U

    2013-01-01

    Abstract Background There is no information on the effects of the breed, gender and age on the cellular content and growth factor (GF) release from equine pure-platelet rich plasma (P-PRP) and pure-platelet rich gel (P-PRG). The objectives of this study were: 1) to compare the cellular composition of P-PRP with whole blood and platelet poor plasma (PPP); 2) to compare the concentration of transforming GF beta 1 (TGF-β1) and platelet derived GF isoform BB (PDGF-BB) between P-PRP treated with n...

  1. Treatment of Burn by Transplanting Fibrinous Membrane with Autologous Epidermal Cells on Raw Surface%自体表皮细胞-纤维蛋白膜创面移植治疗大鼠烧伤

    Institute of Scientific and Technical Information of China (English)

    陈子英; 王晓晔; 崔华雷

    2013-01-01

    目的:观察自体表皮细胞-纤维蛋白膜移植到大鼠烧伤创面治疗皮肤缺损的效果。方法:健康Wistar大鼠20只,随机分成烧伤皮肤缺损造模组和自体表皮细胞-纤维蛋白膜移植治疗组,治疗后计算表皮细胞在纤维蛋白膜上最佳接种密度,观察移植后的各组创面愈合情况、创面伤口的收缩比例等。结果:在纤维蛋白膜上接种表皮细胞的最佳密度为5×104/㎝2,烧伤皮肤缺损造模组创面完全愈合时间平均22.3 d,自体表皮细胞-纤维蛋白膜移植治疗组为18.1 d,造模组创面收缩率为(70±5)%,移植组为(20±5)%(均P<0.05)。结论:自体表皮细胞-纤维蛋白膜可用于覆盖大面积烧伤造成的皮肤缺损,预防创面伤口瘢痕化的形成,减轻创面收缩率,加速皮肤缺损创面的愈合速度。%Objective To observe the treatment effect of skin defect by transplanting fibrinous membrane with autologous epidermal cells to burn wounds in rats, and to look for new ways to treat the burn caused by large area skin defect. Methods Twenty healthy Wistar rats were randomly divided into burn skin defect mod-ule group and fibrinous membrane with autologous epidermal cells transplanting group. The optimum epidermal cells inoculation density on fibrinous membrane was calculated. The wound healing and the wound contraction ratio after fibrinous membrane with autologous epidermal cells transplantation were observed. Results The best density on fibrinous membranes with epidermal cells was 2.5x104/cm2, burn skin defect module group rats wound healed completely spening an average of about 22.3 days, and the fibrinous membrane with autologous epidermal cells transplantation treatment group rats wound healed completely time was about 18.1 days on aver-age; the wound shrinkage rate of burn skin defect module group rats was(70 ± 5)% , and transplantation group rats was(20±5)%. Conclusion The fibrinous membrane

  2. Rehabilitation in Patients with Anterior Cruciate Ligament Reconstruction Using Auxiliary Platelet-Rich Plasma Therapy

    Directory of Open Access Journals (Sweden)

    Albu Daniel – Emil

    2016-06-01

    Full Text Available Background: The main target after successful AnteriorCruciate Ligament (ACL reconstruction is early rehabilitation. New options such as PRP (platelet rich plasma may improve clinical outcomes.

  3. Platelet-rich plasma with sodium hyaluronate in repair of rabbit knee osteoarthritis

    Institute of Scientific and Technical Information of China (English)

    Ji Heng-dong; Huo Xiao-yan; Zhang Hou-qing; Wang Yu-shan; Shi Xuan; Huo Lei

    2015-01-01

    BACKGROUND: Studies have shown that sodium hyaluronate inhibits cartilage damage in osteoarthritis and accelerates regeneration of cartilage cels, to stabilize and repair the articular cartilage. OBJECTIVE:To investigate the therapeutic effect of sodium-rich plasma combined with platelet-rich plasma (PRP) on rabbit knee osteoarthritis. METHODS:Forty New Zealand white rabbits were randomly divided into five groups, control group, combined group, sodium hyaluronate group, PRP group and model group, and then an osteoarthritis model of the right knee was made in each rabbit. After modeling, sodium hyaluronate+PRP, sodium hyaluronate, autologous PRP and normal saline were givenviathe knee joint cavity in the latter four groups, respectively, once a week for 5 weeks. The control group received no treatment, as normal controls. At 1 week after treatment, ELISA assay was used to detect serum interleukin-1, interleukin-6, tumor necrosis factor-α levels, and changes of the articular cartilage were observed under a light microscope. RESULTS AND CONCLUSION:Compared with the control group, the levels of interleukin-1, interleukin-6 and tumor necrosis factor-α were al increased in the other four groups (P < 0.01). Compared with the model group, the levels of interleukin-1, interleukin-6 and tumor necrosis factor-α were lowered significantly in the combined, sodium hyaluronate and PRP groups (P< 0.01 orP< 0.05), and the most significant decline was in the combined group. Articular cartilage damage was severest in the model group and mildest in the combined group. Experimental findings indicate that intra-articular injection of sodium hyaluronate+PRP can reduce inflammation and protect the articular cartilage in knee osteoarthritis, which is better than a single drug injection.

  4. Concentration of platelets and growth factors in platelet-rich plasma from Goettingen minipigs.

    Science.gov (United States)

    Jungbluth, Pascal; Grassmann, Jan-Peter; Thelen, Simon; Wild, Michael; Sager, Martin; Windolf, Joachim; Hakimi, Mohssen

    2014-01-01

    In minipigs little is known about the concentration of growth factors in plasma, despite their major role in several patho-physiological processes such as healing of fractures. This prompted us to study the concentration of platelets and selected growth factors in plasma and platelet-rich plasma (PRP) preparation of sixteen Goettingen minipigs. Platelet concentrations increased significantly in PRP in comparison to native blood plasma. Generally, significant increase in the concentration of all growth factors tested was observed in the PRP in comparison to the corresponding plasma or serum. Five of the plasma samples examined contained detectable levels of bone morphogenic protein 2 (BMP-2) whereas eleven of the plasma or serum samples contained minimal amounts of vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF-bb) respectively. On the other hand variable concentrations of bone morphogenic protein 7 (BMP-7) and transforming growth factor β1 (TGF-β1) were measured in all plasma samples. In contrast, all PRP samples contained significantly increased amounts of growth factors. The level of BMP-2, BMP-7, TGF-β1, VEGF and PDGF-bb increased by 17.6, 1.5, 7.1, 7.2 and 103.3 fold, in comparison to the corresponding non-enriched preparations. Moreover significant positive correlations were found between platelet count and the concentrations of BMP-2 (r=0.62, pVEGF (r=0.46, pplatelet-rich plasma of minipigs which might thus serve as a source of autologous growth factors. PMID:26504722

  5. T2 mapping and dGEMRIC after autologous chondrocyte implantation with a fibrin-based scaffold in the knee: Preliminary results

    Energy Technology Data Exchange (ETDEWEB)

    Domayer, S.E. [Department of Orthopedics, Medical University of Vienna, Waehringer Guertel 18-20, A 1090 Vienna (Austria); MR Centre of Excellence, Department of Radiodiagnostics, Medical University of Vienna, Lazarettgasse 14, A-1090 Vienna (Austria)], E-mail: stephan.domayer@meduniwien.ac.at; Welsch, G.H. [MR Centre of Excellence, Department of Radiodiagnostics, Medical University of Vienna, Lazarettgasse 14, A-1090 Vienna (Austria); Nehrer, S. [Centre of Regenerative Medicine, Danube University of Krems, Dr.-Karl-Dorrek-Strasse, 30 A-3500 Krems (Austria)], E-mail: stefan.nehrer@donau-uni.ac.at; Chiari, C.; Dorotka, R. [Department of Orthopedics, Medical University of Vienna, Waehringer Guertel 18-20, A 1090 Vienna (Austria); Szomolanyi, P. [MR Centre of Excellence, Department of Radiodiagnostics, Medical University of Vienna, Lazarettgasse 14, A-1090 Vienna (Austria); Institute of Measurement Science, Slovak Academy of Sciences, Dubravska cesta 9, 841 04 Bratislava (Slovakia); Mamisch, T.C. [Department of Orthopedics, Inselspital, University of Bern, 3010 Bern (Switzerland); Yayon, A. [ProChon Biotech Ltd., Weizmann Science Park, Nes Ziona (Israel); Trattnig, S. [MR Centre of Excellence, Department of Radiodiagnostics, Medical University of Vienna, Lazarettgasse 14, A-1090 Vienna (Austria)], E-mail: siegfried.trattnig@meduniwien.ac.at

    2010-03-15

    Objective: To assess repair tissue (RT) after the implantation of BioCart{sup TM}II, an autologous chondrocyte implantation (ACI) technique with a fibrin-hyaluronan polymer as scaffold. T2 mapping and delayed Gadolinium Enhanced Magnetic Resonance Imaging of Cartilage (dGEMRIC) were used to gain first data on the biochemical properties of BioCart{sup TM}II RT in vivo. Methods: T2 mapping and dGEMRIC were performed at 3 T in five patients (six knee joints) who had undergone ACI 15-27 months before. T2 maps were obtained using a pixel wise, mono-exponential non-negative least squares fit analysis. For quantitative T1 mapping a dual flip angle 3D GRE sequence was used and T1 maps were calculated pre- and post-contrast using IDL software. Subsequent region of interest analysis was carried out in comparison with morphologic MRI. Results: A spatial variation of T2 values in both hyaline, normal cartilage (NC) and RT was found. Mean RT T2 values and mean NC T2 values did not differ significantly. Relative T2 values were calculated from global RT and NC T2 and showed a small range (0.84-1.07). The relative delta relaxation rates (r{delta}R1) obtained from the T1 maps had a wider range (0.77-4.91). Conclusion: T2 mapping and dGEMRIC provided complementary information on the biochemical properties of the repair tissue. BioCart{sup TM}II apparently can provide RT similar to hyaline articular cartilage and may become a less-invasive alternative to ACI with a periosteal flap.

  6. A prospective randomized controlled study on topical autologous platelet-rich plasma gel for the treatment of diabetic non-healing dermal ulcers%自体富血小板凝胶与标准疗法治疗糖尿病难治性皮肤溃疡的随机对照研究

    Institute of Scientific and Technical Information of China (English)

    王艳; 王椿; 何利平; 杨阎峙; 吕丽芳; 刘关键; 冉兴无

    2009-01-01

    目的 评价自体富血小板凝胶(APG)治疗糖尿病难治性皮肤溃疡的有效性和安全性.方法 2007年4月至2008年2月在我科住院治疗的糖尿病皮肤溃疡患者46例,随机分入APG治疗组(23例)和标准治疗组(23例).两组患者均使用胰岛素控制血糖,严格控制血压、血脂,同时给予抗血小板、改善微循环、抗感染等一般治疗和局部溃疡创面标准处理.APG治疗组患者根据创面体积/面积用自制APG并外敷速愈乐敷料;标准治疗组直接外敷速愈乐敷料.疗程直至创面愈合、行皮瓣移植手术或12周满.结果 除标准治疗组失访1例,其余患者均完成试验.APG治疗组溃疡愈合率为22/23(95.7%),总有效率为100%;标准治疗组溃疡愈合率为13/23(56.5%),总有效率为73.9%,两组比较差异有统计学意义(P值分别为0.002,0.009); Kaplan-Meier溃疡时间愈合曲线和窦道闭合曲线分析提示P<0.05,显示APG治疗明显优于标准治疗;治疗过程中未发现APG治疗相关副作用发生.结论 APG治疗糖尿病难治性皮肤溃疡优于标准治疗法,且治疗伴窦道的溃疡更有优势;APG治疗糖尿病难治性皮肤溃疡是安全、有效、可行的.%Objective To compare the safety and effectiveness between treatments with autologous platelet gel (APG) versus standard care for treating refractory diabetic dermal ulcers.Methods The 46 patients with proved nonhealing diabetic dermal ulcers were enrolled. Eligible for the study were patients with grade II/III ulcers according to Wagner, lasting for at least 2 weeks and with no signs of infection at recruitment.Patients were given their informed consent document and randomly assigned to two groups: standard care (ST, n=23) or standard care plus topic application of APG (APG, n=23) for twelve weeks.The treatment of blood glucose, blood pressure and lipids was optimized and the empiric antibiotic treatment was further adjusted according to the results of culture and

  7. Platelet rich plasma injection grafts for musculoskeletal injuries: a review

    OpenAIRE

    Sampson, Steven; Gerhardt, Michael; Mandelbaum, Bert

    2008-01-01

    In Europe and the United States, there is an increasing prevalence of the use of autologous blood products to facilitate healing in a variety of applications. Recently, we have learned more about specific growth factors, which play a crucial role in the healing process. With that knowledge there is abundant enthusiasm in the application of concentrated platelets, which release a supra-maximal quantity of these growth factors to stimulate recovery in non-healing injuries. For 20 years, the app...

  8. Chronic Plantar Fasciitis: Effect of Platelet-Rich Plasma, Corticosteroid, and Placebo.

    Science.gov (United States)

    Mahindra, Pankaj; Yamin, Mohammad; Selhi, Harpal S; Singla, Sonia; Soni, Ashwani

    2016-01-01

    Plantar fasciitis is a common cause of heel pain. It is a disabling disease in its chronic form. It is a degenerative tissue condition of the plantar fascia rather than an inflammation. Various treatment options are available, including nonsteroidal anti-inflammatory drugs, corticosteroid injections, orthosis, and physiotherapy. This study compared the effects of local platelet-rich plasma, corticosteroid, and placebo injections in the treatment of chronic plantar fasciitis. In this double-blind study, patients were divided randomly into 3 groups. Local injections of platelet-rich plasma, corticosteroid, or normal saline were given. Patients were assessed with the visual analog scale for pain and with the American Orthopaedic Foot and Ankle Society (AOFAS) Ankle and Hindfoot score before injection, at 3 weeks, and at 3-month follow-up. Mean visual analog scale score in the platelet-rich plasma and corticosteroid groups decreased from 7.44 and 7.72 preinjection to 2.52 and 3.64 at final follow-up, respectively. Mean AOFAS score in the platelet-rich plasma and corticosteroid groups improved from 51.56 and 55.72 preinjection to 88.24 and 81.32 at final follow-up, respectively. There was a significant improvement in visual analog scale score and AOFAS score in the platelet-rich plasma and corticosteroid groups at 3 weeks and at 3-month follow-up. There was no significant improvement in visual analog scale score or AOFAS score in the placebo group at any stage of the study. The authors concluded that local injection of platelet-rich plasma or corticosteroid is an effective treatment option for chronic plantar fasciitis. Platelet-rich plasma injection is as effective as or more effective than corticosteroid injection in treating chronic plantar fasciitis. PMID:26913766

  9. Single injection of platelet-rich plasma as a novel treatment of carpal tunnel syndrome

    Directory of Open Access Journals (Sweden)

    Michael Alexander Malahias

    2015-01-01

    Full Text Available Both in vitro and in vivo experiments have confirmed that platelet-rich plasma has therapeutic effects on many neuropathies, but its effects on carpal tunnel syndrome remain poorly understood. We aimed to investigate whether single injection of platelet-rich plasma can improve the clinical symptoms of carpal tunnel syndrome. Fourteen patients presenting with median nerve injury who had suffered from mild carpal tunnel syndrome for over 3 months were included in this study. Under ultrasound guidance, 1-2 mL of platelet-rich plasma was injected into the region around the median nerve at the proximal edge of the carpal tunnel. At 1 month after single injection of platelet-rich plasma, Visual Analogue Scale results showed that pain almost disappeared in eight patients and it was obviously alleviated in three patients. Simultaneously, the disabilities of the arm, shoulder and hand questionnaire showed that upper limb function was obviously improved. In addition, no ultrasonographic manifestation of the carpal tunnel syndrome was found in five patients during ultrasonographic measurement of the width of the median nerve. During 3-month follow-up, the pain was not greatly alleviated in three patients. These findings show very encouraging mid-term outcomes regarding use of platelet-rich plasma for the treatment of carpal tunnel syndrome.

  10. Effectiveness and efficiency of platelet rich plasma in the treatment of diabetic ulcers.

    Science.gov (United States)

    Cobos, Raquel; Aizpuru, Felipe; Parraza, Naiara; Anitua, Eduardo; Orive, Gorka

    2015-01-01

    "There is a growing body of evidence suggesting that wound healing in chronic diabetic foot ulcers is growth factor dependent, and that the therapeutic delivery of these growth factors to wounds topically, has the potential ability to accelerate wound healing in conjunction with conventional wound care". There is, however, confusion about the utility of platelet rich plasma because the studies that have evaluated them use a wide range of products (different platelet and leukocyte concentrations, different techniques and frequencies of application, very heterogeneous simple, and different endpoints) making almost impossible to compare data and draw conclusions. In this study, we have analyzed the different platelet rich plasma products from a new perspective: cost-efficiency. According to our data, we observe that platelet rich plasma is a cost-effective option that allows faster healing of ulcers, and that should be taken into account in patients with long evolution ulcers. PMID:25934972

  11. Effect of platelet-rich plasma in the treatment of periodontal intrabony defects in humans

    Institute of Scientific and Technical Information of China (English)

    OUYANG Xiang-ying; QIAO Jing

    2006-01-01

    Background Platelet-rich plasma (PRP) is a kind of natural source of autologous growth factors, and has been used successfully in medical community. However, the effect of PRP in periodontal regeneration is not clear yet.This study was designed to evaluate the effectiveness of PRP as an adjunct to bovine porous bone mineral (BPBM) graft in the treatment of human intrabony defects.Methods Seventeen intrabony defects in 10 periodontitis patients were randomly treated either with PRP and BPBM (test group, n=9) or with BPBM alone (control group, n=8). Clinical parameters were evaluated including changes in probing depth, relative attachment level (measured by Florida Probe and a stent), and bone probing level between baseline and 1 year postoperatively. Standardized periapical radiographs of each defect were taken at baseline, 2 weeks, and 1 year postoperatively, and analyzed by digital subtraction radiography (DSR).Results Both treatment modalities resulted in significant attachment gain, reduction of probing depth, and bone probing level at 1-year post-surgery compared to baseline. The test group exhibited statistically significant improvement compared to the control sites in probing depth reduction: (4.78 ± 0.95) mm versus (3.48±0.41) mm (P<0.01); clinical attachment gain: (4.52± 1.14) mm versus (2.85 ±0.80) mm (P<0.01);bone probing reduction:(4.56±1.04) mm versus (2.88±0.79) mm (P<0.01); and defect bone fill: (73.41±14.78)% versus (47.32±11.47)% (P<0.01). DSR analysis of baseline and 1 year postoperatively also showed greater radiographic gains in alveolar bone mass in the test group than in the control group: gray increase (580 ±50) grays versus (220 ± 32)grays (P=0.0001);area with increased gray were (5.21±1.25) mm2 versus (3.02±1.22) mm2 (P=0.0001).Conclusions The treatment with a combination of PRP and BPBM led to a significantly favorable clinical improvement in periodontal intrabony defects compared to using BPBM alone. Further studies are

  12. Mesotherapy and platelet-rich plasma for the treatment of hair loss

    Directory of Open Access Journals (Sweden)

    Gonca Gökdemir

    2014-06-01

    Full Text Available Hair loss often significant impact on quality of life, including loss of self-confidence and self-esteem. However, treatment of hair loss is frustrating for both patients and doctors. Mesotherapy and platelet-rich plasma have recently become advertised method for the treatment of different types of alopecia. The efficacy of these methods in hair loss is controversial in view of lack of documented evidence. It was reviewed the data about the efficacy, safety and treatment protocols of mesotherapy and platelet-rich plasma in patients with hair loss.

  13. 富血小板血浆在骨科领域的应用与前景%Application and Prospects of Platelet-rich Plasma in Orthopedics

    Institute of Scientific and Technical Information of China (English)

    许生领

    2011-01-01

    富血小板血浆(PRP)是自体血小板的浓缩体,含有丰富的血小板,其所含的多种生长因子,其比例与体内正常比例相似,有助于促进骨、软骨的缺损修复,引导软组织再生及预防感染等.现就相关文献回顾探讨富血小板血浆的分离和制备、成分和作用,以及在骨科领域中的应用、作用机制和展望等,为PRP在骨科领域中的应用提供依据.%Platelet-rich plasma ( PRP ) is the autologous platelet concentrates, rich in platelets, which contains a variety of growth factors, contributes to bone, cartilage defects repair, guides soft tissue regeneration and prevent infection, etc..Here is to review the literature on the separation and preparation of platelet-rich plasma,composition and role,and applications, functional mechanisms and prospects in the field of orthopedic, providing basis for PRP application in orthopedics.

  14. A novel hypothesis: the application of platelet-rich plasma can promote the clinical healing of white-white meniscal tears.

    Science.gov (United States)

    Wei, Li-Cheng; Gao, Shu-Guang; Xu, Mai; Jiang, Wei; Tian, Jian; Lei, Guang-Hua

    2012-08-01

    The white-white tears (meniscus lesion completely in the avascular zone) are without blood supply and theoretically cannot heal. Basal research has demonstrated that menisci are unquestionably important in load bearing, load redistribution, shock absorption, joint lubrication and the stabilization of the knee joint. It has been proven that partial or all-meniscusectomy results in an accelerated degeneration of cartilage and an increased rate of early osteoarthritis. Knee surgeons must face the difficult decision of removing or, if possible, retaining the meniscus; if it is possible to retain the meniscus, surgeons must address the difficulties of meniscal healing. Some preliminary approaches have progressed to improve meniscal healing. However, the problem of promoting meniscal healing in the avascular area has not yet been resolved. The demanding nature of the approach as well as its low utility and efficacy has impeded the progress of these enhancement techniques. Platelet-rich plasma (PRP) is a platelet concentration derived from autologous blood. In recent years, PRP has been used widely in preclinical and clinical applications for bone regeneration and wound healing. Therefore, we hypothesize that the application of platelet-rich plasma for white-white meniscal tears will be a simple and novel technique of high utility in knee surgery.

  15. Effects of Platelet-Rich Plasma (PRP) on a Model of Renal Ischemia-Reperfusion in Rats.

    Science.gov (United States)

    Martín-Solé, Oriol; Rodó, Joan; García-Aparicio, Lluís; Blanch, Josep; Cusí, Victoria; Albert, Asteria

    2016-01-01

    Renal ischemia-reperfusion injury is a major cause of acute renal failure, causing renal cell death, a permanent decrease of renal blood flow, organ dysfunction and chronic kidney disease. Platelet-rich plasma (PRP) is an autologous product rich in growth factors, and therefore able to promote tissue regeneration and angiogenesis. This product has proven its efficacy in multiple studies, but has not yet been tested on kidney tissue. The aim of this work is to evaluate whether the application of PRP to rat kidneys undergoing ischemia-reperfusion reduces mid-term kidney damage. A total of 30 monorrenal Sprague-Dawley male rats underwent renal ischemia-reperfusion for 45 minutes. During ischemia, PRP (PRP Group, n = 15) or saline solution (SALINE Group, n = 15) was administered by subcapsular renal injection. Control kidneys were the contralateral organs removed immediately before the start of ischemia in the remaining kidneys. Survival, body weight, renal blood flow on Doppler ultrasound, kidney weight, kidney volume, blood biochemistry and histopathology were determined for all subjects and kidneys, as applicable. Correlations between these variables were searched for. The PRP Group showed significantly worse kidney blood flow (p = 0.045) and more histopathological damage (pkidney volume, kidney weight, renal blood flow, histology, and serum levels of creatinine and urea. Our study provides the first evidence that treatment with PRP results in the deterioration of the kidney's response to ischemia-reperfusion injury. PMID:27551718

  16. Do autologous blood and PRP injections effectively treat tennis elbow?

    Science.gov (United States)

    Widstrom, Luke; Slattengren, Andrew

    2016-09-01

    Both approaches reduce pain, but the improvement with platelet-rich plasma (PRP) is not clinically meaningful. Autologous blood injections (ABIs) are more effective than corticosteroid injections for reducing pain and disability in patients with tennis elbow in both the short and long term.

  17. Clinical evaluation of the effect of platelet rich plasma on the coronally advanced flap root coverage procedure

    Directory of Open Access Journals (Sweden)

    Sharashchandra Madhavarao Biradar

    2015-01-01

    Full Text Available Background: Coronally advanced flap (CAF has been shown to effectively treat gingival recession. Platelet rich plasma (PRP, containing autologous growth factors, has been shown to promote soft tissue healing. The aim of this clinical study was to determine whether the addition of an autologous PRP to a CAF when compared to a CAF alone would improve the clinical outcome for treatment of multiple gingival recessions. Materials and Methods: Thirty patients with Miller′s class I and class II buccal recession defect were randomly assigned to control (CAF alone or test (CAF with PRP groups. Recession depth (RD, recession width (RW, probing depth (PD, clinical attachment level (CAL, width of keratinized tissue, wound healing index, percentage of root coverage was assessed. Patients were followed at 2, 4, 12 and 16 weeks post-surgery. Statistical analysis for intra and inter group comparisons was done using Wilcoxon sign rank and Wilcoxon rank sum tests respectively. P < 0.05 denoted statistical significance. Results: The differences between the test and control groups were not significant with respect to all the clinical variables. The RD at 16 weeks was significantly reduced from 2.88 ± 0.69 to 0.76 ± 0.24 mm in control group (P < 0.05 and from 2.95 ± 0.43 to 0.76 ± 0.35 mm in the test group (P < 0.05. The mean percentage of root coverage was 73.1 ± 7.3 in the control group and 75.0 ± 8.3 in test group (P < 0.05. The CAL gain was 3.17 ± 0.84 mm within the control group and 3.17 ± 0.79 mm within test group. Conclusion: Within the limitations of this study, PRP with CAF can provide an early healing of soft tissues, but does not provide clinically measurable improvement in the final therapeutics outcome in CAF.

  18. Platelet-Rich Plasma Increases the Levels of Catabolic Molecules and Cellular Dedifferentiation in the Meniscus of a Rabbit Model

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    Hye-Rim Lee

    2016-01-01

    Full Text Available Despite the susceptibility to frequent intrinsic and extrinsic injuries, especially in the inner zone, the meniscus does not heal spontaneously owing to its poor vascularity. In this study, the effect of platelet-rich plasma (PRP, containing various growth factors, on meniscal mechanisms was examined under normal and post-traumatic inflammatory conditions. Isolated primary meniscal cells of New Zealand white (NZW rabbits were incubated for 3, 10, 14 and 21 days with PRP(−, 10% PRP (PRP(+, IL(+ or IL(+PRP(+. The meniscal cells were collected and examined using reverse-transcription polymerase chain reaction (RT-PCR. Culture media were examined by immunoblot analyses for matrix metalloproteinases (MMP catabolic molecules. PRP containing growth factors improved the cellular viability of meniscal cells in a concentration-dependent manner at Days 1, 4 and 7. However, based on RT-PCR, meniscal cells demonstrated dedifferentiation, along with an increase in type I collagen in the PRP(+ and in IL(+PRP(+. In PRP(+, the aggrecan expression levels were lower than in the PRP(− until Day 21. The protein levels of MMP-1 and MMP-3 were higher in each PRP group, i.e., PRP(+ and IL(+PRP(+, at each culture time. A reproducible 2-mm circular defect on the meniscus of NZW rabbit was used to implant fibrin glue (control or PRP in vivo. After eight weeks, the lesions in the control and PRP groups were occupied with fibrous tissue, but not with meniscal cells. This study shows that PRP treatment of the meniscus results in an increase of catabolic molecules, especially those related to IL-1α-induced inflammation, and that PRP treatment for an in vivo meniscus injury accelerates fibrosis, instead of meniscal cartilage.

  19. Modification of Pulsed Electric Field Conditions Results in Distinct Activation Profiles of Platelet-Rich Plasma

    Science.gov (United States)

    Frelinger, Andrew L.; Gerrits, Anja J.; Garner, Allen L.; Torres, Andrew S.; Caiafa, Antonio; Morton, Christine A.; Berny-Lang, Michelle A.; Carmichael, Sabrina L.; Neculaes, V. Bogdan; Michelson, Alan D.

    2016-01-01

    Background Activated autologous platelet-rich plasma (PRP) used in therapeutic wound healing applications is poorly characterized and standardized. Using pulsed electric fields (PEF) to activate platelets may reduce variability and eliminate complications associated with the use of bovine thrombin. We previously reported that exposing PRP to sub-microsecond duration, high electric field (SMHEF) pulses generates a greater number of platelet-derived microparticles, increased expression of prothrombotic platelet surfaces, and differential release of growth factors compared to thrombin. Moreover, the platelet releasate produced by SMHEF pulses induced greater cell proliferation than plasma. Aims To determine whether sub-microsecond duration, low electric field (SMLEF) bipolar pulses results in differential activation of PRP compared to SMHEF, with respect to profiles of activation markers, growth factor release, and cell proliferation capacity. Methods PRP activation by SMLEF bipolar pulses was compared to SMHEF pulses and bovine thrombin. PRP was prepared using the Harvest SmartPreP2 System from acid citrate dextrose anticoagulated healthy donor blood. PEF activation by either SMHEF or SMLEF pulses was performed using a standard electroporation cuvette preloaded with CaCl2 and a prototype instrument designed to take into account the electrical properties of PRP. Flow cytometry was used to assess platelet surface P-selectin expression, and annexin V binding. Platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), endothelial growth factor (EGF) and platelet factor 4 (PF4), and were measured by ELISA. The ability of supernatants to stimulate proliferation of human epithelial cells in culture was also evaluated. Controls included vehicle-treated, unactivated PRP and PRP with 10 mM CaCl2 activated with 1 U/mL bovine thrombin. Results PRP activated with SMLEF bipolar pulses or thrombin had similar light scatter profiles, consistent with the

  20. Platelet-rich plasma in bone repair of irradiated tibiae of Wistar rats

    Energy Technology Data Exchange (ETDEWEB)

    Gumieiro, Emne Hammoud; Abrahao, Marcio; Jahn, Ricardo Schmitutz, E-mail: gumieiro@uol.com.b [Universidade Federal de Sao Paulo (UNIFESP-EPM), SP (Brazil). Dept. of Otorhinolaringology and Head and Neck Surgery; Segretto, Helena [Universidade Federal de Sao Paulo (UNIFESP-EPM), SP (Brazil). Dept. of Oncology; Alves, Maria Tereza de Seixas [Universidade Federal de Sao Paulo (UNIFESP-EPM), SP (Brazil). Dept. of Patology; Nannmark, Ulf [The Sahlgrenska Academy of Goeteborg Univ. (Sweden). Inst. for Clinical Sciences. Dept. of Anatomy and Cell Biology; Granstroem, Goesta [Goeteborg Univ. (Sweden). Dept. of Otolaryngology, Head and Neck Surgery; Dib, Luciano Lauria [Universidade Paulista (UNIP), Sao Paulo, SP (Brazil). Faculty of Dentistry. Dept. of Stomatology

    2010-05-15

    Purpose: to evaluate the influence of PRP addition on bone repair of circular defects created in irradiated tibiae of rats by histometric analysis. Methods: sixty male Wistar rats had the right tibiae irradiated with 30 Gy. After 30 days monocortical defects were created and platelet-rich plasma as applied in 30 rats. In the control group defects were created but not filled. The animals were sacrificed after 4, 7, 14, 21, 56 and 84 days and the tibiae removed for histological processing. Results: there was a tendency in the PRP group to increased bone neoformation from 14-days to 84-days; in the control group increased bone neoformation was not seen after 21 days or later. Conclusion: the addition of platelet-rich plasma had a beneficial effect in the initial cellular regeneration period and enhanced bone formation in later periods when compared to control. (author)

  1. Mechanisms of Ascorbyl Radical Formation in Human Platelet-Rich Plasma

    OpenAIRE

    Kou-Gi Shyu; Chao-Chien Chang; Yu-Chieh Yeh; Joen-Rong Sheu; Duen-Suey Chou

    2014-01-01

    Recently, many clinical reports have suggested that the ascorbyl free radical (Asc∙) can be treated as a noninvasive, reliable, real-time marker of oxidative stress, but its generation mechanisms in human blood have rarely been discussed. In this study, we used upstream substances, enzyme inhibitors, and free radical scavengers to delineate the mechanisms of Asc∙ formation in human platelet-rich plasma (PRP). Our results show that the doublet signal was detected in PRP samples by using electr...

  2. Platelet-Rich Plasma in Androgenic Alopecia: Myth or an Effective Tool

    OpenAIRE

    Khatu, Swapna S; More, Yuvraj E; Gokhale, Neeta R; Chavhan, Dipali C; Nitin Bendsure

    2014-01-01

    Platelet-rich plasma (PRP) has become a newer method for the treatment of various types of alopecia. In this prospective study, safety, efficacy and feasibility of PRP injections in treating androgenic alopecia were assessed. Eleven patients suffering from hair loss due to androgenic alopecia and not responding to 6 months treatment with minoxidil and finasteride were included in this study. The hair pull test was performed before every treatment session. A total volume of 2-3 cc PRP was inje...

  3. Assessment of Vascular Endothelial Growth Factor in Fresh versus Frozen Platelet Rich Plasma

    OpenAIRE

    Nada Hosny; Fikry Goubran; Basma BadrEldin Hasan; Noha Kamel

    2015-01-01

    Platelet rich plasma (PRP) is hemoconcentration with platelets concentration above baseline values and high concentration of many growth factors. The aim of this study was to assess freezing effect on vascular endothelial growth factor (VEGF) release from PRP using two different activation methods to simplify its use in different clinical applications. PRP was prepared using two-centrifugation steps method from 12 qualified blood donors. VEGF concentrations were measured in fresh PRP and afte...

  4. Concentration of platelets and growth factors in platelet-rich plasma from Goettingen minipigs

    OpenAIRE

    Jungbluth, P; Grassmann, JP; Thelen, S; Wild, M.; Sager, M; Windolf, J.; M Hakimi

    2014-01-01

    In minipigs little is known about the concentration of growth factors in plasma, despite their major role in several patho-physiological processes such as healing of fractures. This prompted us to study the concentration of platelets and selected growth factors in plasma and platelet-rich plasma (PRP) preparation of sixteen Goettingen minipigs. Platelet concentrations increased significantly in PRP in comparison to native blood plasma. Generally, significant increase in the concentration of a...

  5. Repair of Segmental Load-Bearing Bone Defect by Autologous Mesenchymal Stem Cells and Plasma-Derived Fibrin Impregnated Ceramic Block Results in Early Recovery of Limb Function

    Directory of Open Access Journals (Sweden)

    Min Hwei Ng

    2014-01-01

    Full Text Available Calcium phosphate-based bone substitutes have not been used to repair load-bearing bone defects due to their weak mechanical property. In this study, we reevaluated the functional outcomes of combining ceramic block with osteogenic-induced mesenchymal stem cells and platelet-rich plasma (TEB to repair critical-sized segmental tibial defect. Comparisons were made with fresh marrow-impregnated ceramic block (MIC and partially demineralized allogeneic bone block (ALLO. Six New Zealand White female rabbits were used in each study group and three rabbits with no implants were used as negative controls. By Day 90, 4/6 rabbits in TEB group and 2/6 in ALLO and MIC groups resumed normal gait pattern. Union was achieved significantly faster in TEB group with a radiological score of 4.50 ± 0.78 versus ALLO (1.06 ± 0.32, MIC (1.28 ± 0.24, and negative controls (0. Histologically, TEB group scored the highest percentage of new bone (82% ± 5.1% compared to ALLO (5% ± 2.5% and MIC (26% ± 5.2%. Biomechanically, TEB-treated tibiae achieved the highest compressive strength (43.50 ± 12.72 MPa compared to those treated with ALLO (15.15 ± 3.57 MPa and MIC (23.28 ± 6.14 MPa. In conclusion, TEB can repair critical-sized segmental load-bearing bone defects and restore limb function.

  6. Effects of Calcium Sulfate Combined with Platelet-rich Plasma on Restoration of Long Bone Defect in Rabbits

    Institute of Scientific and Technical Information of China (English)

    Hua Chen; Xin-Ran Ji; Qun Zhang; Xue-Zhong Tian; Bo-Xun Zhang; Pei-Fu Tang

    2016-01-01

    Background:The treatment for long bone defects has been a hot topic in the field of regenerative medicine.This study aimed to evaluate the therapeutic effects of calcium sulfate (CS) combined with platelet-rich plasma (PRP) on long bone defect restoration.Methods:A radial bone defect model was constructed through an osteotomy using New Zealand rabbits.The rabbits were randomly divided into four groups (n =10 in each group):a CS combined with PRP (CS-PRP) group,a CS group,a PRP group,and a positive (recombinant human bone morphogenetic protein-2) control group.PRP was prepared from autologous blood using a two-step centrifugation process.CS-PRP was obtained by mixing hemihydrate CS with PRP.Radiographs and histologic micrographs were generated.The percentage of bone regenerated bone area in each rabbit was calculated at 10 weeks.One-way analysis of variance was performed in this study.Results:The radiographs and histologic micrographs showed bone restoration in the CS-PRP and positive control groups,while nonunion was observed in the CS and PRP groups.The percentages of bone regenerated bone area in the CS-PRP (84.60 ± 2.87%) and positive control (52.21 ± 4.53%) groups were significantly greater than those in the CS group (12.34 ± 2.17%) and PRP group (16.52 ± 4.22%) (P < 0.001).In addition,the bone strength of CS-PRP group (43.l 0 ± 4.10%) was significantly greater than that of the CS group (20.10 ± 3.70%) or PRP group (25.10 ± 2.10%) (P < 0.001).Conclusion:CS-PRP functions as an effective treatment for long bone defects through stimulating bone regeneration and enhancing new bone strength.

  7. Study of platelet-rich plasma injections in the treatment of androgenetic alopecia through an one-year period

    Directory of Open Access Journals (Sweden)

    Maria-Angeliki Gkini

    2014-01-01

    Full Text Available Background: Platelet-rich plasma (PRP is defined as an autologous concentration of plasma with a greater count of platelets than that of whole blood. Its action depends on the released growth factors from platelets. It has been investigated and used in numerous fields of medicine. Recently, PRP has received growing attention as a potential therapeutic tool for hair loss. Aims: To evaluate the efficacy and safety of PRP injections in the scalp of patients with androgenetic alopecia. Settings and Design: Prospective cohort study. Materials and Methods: 20 patients, 18 males and 2 females, with androgenetic alopecia were enrolled in the study. PRP was prepared using a single spin method (Regenlab SA. Upon activation, it was injected in the androgen-related areas of scalp. Three treatment sessions were performed with an interval of 21 days and a booster session at 6 months following the onset of therapy. Statistical Analysis: Statistical analysis of the data was performed using the Statistical Package for the Social Sciences (SPSS, version 19.0 (IBM, NY, USA. Results: Hair loss reduced and at 3 months it reached normal levels. Hair density reached a peak at 3 months (170.70 ± 37.81, P < 0.001. At 6 months and at 1 year, it was significantly increased, 156.25 ± 37.75 (P < 0.001 and 153.70 ± 39.92 (P < 0.001 respectively, comparing to baseline. Patients were satisfied with a mean result rating of 7.1 on a scale of 1-10. No remarkable adverse effects were noted. Conclusions: Our data suggest that PRP injections may have a positive therapeutic effect on male and female pattern hair loss without remarkable major side effects. Further studies are needed to confirm its efficacy.

  8. Efficacy of platelet-rich plasma applied to post-extraction retained lower third molar alveoli. A systematic review

    OpenAIRE

    Barona Dorado, Cristina; González Regueiro, Iria; Martín Ares, María; Arias Irimia, Óscar; Martínez González, José María

    2013-01-01

    Dental retentions have a high prevalence among the general population and their removal can involve multiple complications. The use of platelet rich plasma has been proposed in an attempt to avoid these complications, as it contains high growth factors and stimulates diverse biological functions that facilitate the healing of soft and hard tissues. Objectives: To evaluate the available scientific evidence related to the application of platelet-rich plasma in the post-extraction alveoli of a r...

  9. Comparison between the effects of platelet-rich plasma and bone marrow concentrate on defect consolidation in the rabbit tibia

    Directory of Open Access Journals (Sweden)

    Marco Antonio Batista

    2011-01-01

    Full Text Available OBJECTIVE: To perform a comparative analysis of the effects of platelet-rich plasma and centrifuged bone marrow aspirate on the induction of bone healing in rabbits. METHOD: Twenty adult, male New Zealand rabbits were randomly separated into two equal groups, and surgery was performed to create a bone defect (a cortical orifice 3.3 mm in diameter in the proximal metaphysis of each rabbit's right tibia. In the first group, platelet-rich plasma was implanted in combination with β-tricalcium phosphate (platelet-rich plasma group, and in the second group, centrifuged bone marrow in combination with β-tricalcium phosphate (centrifuged bone marrow group was implanted. After a period of four weeks, the animals were euthanized, and the tibias were evaluated using digital radiography, computed tomography, and histomorphometry. RESULTS: Seven samples from each group were evaluated. The radiographic evaluation confirmed the absence of fractures in the postoperative limb and identified whether bone consolidation had occurred. The tomographic evaluation revealed a greater amount of consolidation and the formation of a greater cortical bone thickness in the platelet-rich plasma group. The histomorphometry revealed a greater bone density in the platelet-rich plasma group compared with the centrifuged bone marrow group. CONCLUSION: After four weeks, the platelet-rich plasma promoted a greater amount of bone consolidation than the bone marrow aspirate concentrate.

  10. Is Platelet-rich plasma superior to whole blood in the management of chronic tennis elbow: one year randomized clinical trial

    Science.gov (United States)

    2014-01-01

    Background Lateral humeral epicondylitis, or ‘tennis elbow’, is a common condition with a variety of treatment options. Platelet-rich plasma (PRP) and Autologous Whole Blood (AWB) represent new therapeutic options for chronic tendinopathies including tennis elbow. The aim of the present study was to compare the long term effects of PRP versus autologous whole blood local injection in patients with chronic tennis elbow. Methods Seventy six patients with chronic lateral humeral epicondylitis with duration of symptoms more than 3 months were included in this study and randomized into 2 groups. Group 1 was treated with a single injection of 2 mL of autologous leukocyte rich PRP (4.8 times of plasma) and group 2 with 2 mL of AWB. Tennis elbow strap, stretching and strengthening exercises were administered for both groups. Pain and functional improvements were assessed using visual analogue scale (VAS), Mayo score (modified Mayo Clinic performance index for the elbow) and pressure pain threshold (PPT) at 0, 4, 8 weeks and 6 and 12 months. Results All pain variables including VAS, PPT and Mayo scores improved significantly in both groups at each follow up intervals compared to baseline. No statistically significant difference was noted between groups regarding pain, functional scores and treatment success rates in all follow up examinations (P >0/05). Conclusion PRP and autologous whole blood injections are both effective methods to treat chronic lateral epicondylitis and their efficacy persisted during long term follow up. PRP was not superior to AWB in long term follow up. PMID:24635909

  11. Preliminary separation of the growth factors in platelet-rich plasma: effects on the proliferation of human marrow-derived mesenchymal stem cells

    Institute of Scientific and Technical Information of China (English)

    HUANG Qian; WANG Yun-dan; WU Tao; JIANG Shan; HU Yan-ling; PEI Guo-xian

    2009-01-01

    Background Platelet-rich plasma (PRP) as a storage vehicle of growth factors has been successfully used in clinical applications, but in most cases the platelets were autologous. However, the large volume of blood withdrawn has detrimental effects on patients with anemia or poor general health. To overcome these limitations, this study was designed to separate the growth factors in homologous platelet-rich plasma. Methods The gel chromatography with Superdex-75 column was applied to separate PRP supernatants into 4 major fractions. Then the four fractions were vacuumed freeze-dried and re-dissolved in phosphate buffered saline. Proteins concentrations in PRP and in four fractions were detected by bicinchoninic acid protein assay; platelet derived growth factor-AB (PDGF-AB) and transforming growth factor 131 (TGF-β1) levels were determined by sandwich enzyme-linked immunosorbent assays. The effects of fractions on the proliferation of human marrow-derived mesenchymal stem cells (MSCs) were determined by 3-(4, 5- dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Results PRP supernatants were separated into four major fractions by gel chromatography. The proteins recovery was 96.72%. Of the four fractions, fraction B contained the highest TGF-β1 and PDGF-AB levels, and the highest proteins concentrations. Cell proliferation curves of MSC demonstrated that fraction B and C induced a remarkable increase of MTT values compared to the untreated culture (P 0.05). Fraction A and D showed no significant difference to the negative control group (P >0.05). Conclusions The growth factors in PRP supernatants could be preliminarily separated into four fractions by gel chromatography, and the freeze-drying fractions retained the biological activity of growth factors. The growth factors were mostly presented in fraction B and C, and they promoted cell proliferation effectively.

  12. Study of Platelet Rich Plasma Injections in Patients of Tendinopathy in South Gujarat Population

    Directory of Open Access Journals (Sweden)

    Manish Patel

    2016-06-01

    Full Text Available Introduction: Tendinopathy is a major medical problem associated with sports and physical activity in active people over 25 years of age. We study about the effect of PRP in the patients of chronic Tendinopathy. Methods: The patients of chronic tendinopathy come to OPD at government medical college, surat who failed medical treatment for last 3 months. After getting informed consent platelet rich plasma is prepared from patients own blood. After giving platelet rich plasma injection, patient is advised to take rest for 3 weeks with analgesics. Physiotherapy is started after 3 weeks of injection as this is causing pain for first 3 weeks. Patients are advised to join their duty after 3 weeks of injection. All the patients were followed up in OPD at 3 weeks, 6 weeks, 3 months and 6 months. At every follow up, range of motion, visual analogue scale and functional activity score recorded. Results: The follow up shows that most of the patients do not get relief within 3 weeks after injection. Follow up shows that 16 patients out of 50 got relief within 6 weeks after injection. Result shows that 46 patients out of 50 get relief within 6 months after injection. That means 94% of patients are having relief within 6 months of injection. Conclusion: The findings of this study shows that platelet rich plasma injection under ultrasound guidance at the tendon is effective mode of treatment for patients and takes time but result in gradual decrease in symptoms. [Natl J Med Res 2016; 6(2.000: 191-195

  13. Platelet-rich plasma as a novel treatment for lichen planopillaris.

    Science.gov (United States)

    Bolanča, Željana; Goren, Andy; Getaldić-Švarc, Biserka; Vučić, Majda; Šitum, Mirna

    2016-07-01

    Treatment of lichen planopillaris (LPP) remains a significant challenge due to the irreversible damage inflicted on hair follicles combined with the low efficacy of existing treatments. We hypothesized that growth factors released by the use of platelet-rich plasma (PRP) may arrest the development of LPP. To test our hypothesis, we treated an LPP patient that has failed previous treatments with a new PRP regimen. Following PRP treatment and six months follow-up, the patient experienced complete regression of itching and hair shedding. To the best of our knowledge, this is the first report of successful treatment of LPP with a PRP regimen. PMID:26988129

  14. Platelet activation: ultrastructure and morphometry in platelet-rich plasma of horses

    OpenAIRE

    Bruna M. Zandim; Maria V. de Souza; Pablo C. Magalhães; Laércio dos A. Benjamin; Leandro Maia; Aécio C. de Oliveira; José de O. Pinto; José I. Ribeiro Júnior

    2012-01-01

    This study was conducted to investigate the activation ability of the platelet-rich plasma (PRP) by pharmacological agents, as well as to verify the need or not of this activation for therapeutic use. The PRP was obtained from four healthy crossbred geldings aged 13 to 16 years (15±1years), and was processed for observation and quantification of the platelet morphology by using the transmission electron microscopy. All PRP samples were activated with 10% calcium chloride (CaCl2) solution, pur...

  15. Treatment of glabella skin necrosis following injection of hyaluronic acid filler using platelet-rich plasma.

    Science.gov (United States)

    Kang, Boo Kyoung; Kang, In Jung; Jeong, Ki Heon; Shin, Min Kyung

    2016-01-01

    Hyaluronic acid (HA) fillers have been widely used for soft-tissue augmentation. However, there can be various complications following HA filler injection. Skin necrosis is rare but one of the most disastrous side effects that, if not treated promptly and effectively, can result in permanent and potentially disfiguring scarring. Thus, early proper management is important. Herein we report a patient who experienced tissue necrosis of the glabellar area after receiving filler injections that was successfully treated using platelet-rich plasma and provide full follow-up clinical photographs. PMID:26052808

  16. Experimental study of the effect of platelet-rich plasma on osteogenesis in rabbit

    Institute of Scientific and Technical Information of China (English)

    张长青; 袁霆; 曾炳芳

    2004-01-01

    @@ Platelet-rich plasma (PRP) is produced from a patient's own blood by centrifugation, and PRP contains several kinds of growth factors in high concentration such as platelet derived growth factor (PDGF), transforming growth factor-β (TGF-β), vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF), epidermal growth factor (EGF), and so on.1 These growth factors have proved to offer an improved quality and speed of healing for both hard and soft tissue.2 In this study, PRP compounded with porous bioceramic was used to repair a bone defect in rabbit radius. The radiographic and histological qualitative and quantitative observations were performed to evaluate osteogenesis.

  17. Osteobiology: newest bone organ topics and the platelet-rich plasma treatment.

    Directory of Open Access Journals (Sweden)

    Ananias García Cardona

    2007-11-01

    Full Text Available The bone is a dynamic tissue taht provides mechanical support, physical protection, storage site for minerals, and enables genesis movement. The bone biology (osteobiology is regulated by the balance betqeen osteoblastic formation and osteoclatic resorption. the skeletal bone homeostasis is influenced by components of the bone marrow organ, neuroendocrine system and hemato-inmmune system. The purpose of this review is to describe the biodynamic of the bone organ, and actual terapeutics with platelet-rich plasma in guide bone regeneration, a co-surgical method employed to increase the quantity and quality of the bone.

  18. The Security of Platelet-rich Plasma in Sports-relatedInjuries

    Institute of Scientific and Technical Information of China (English)

    Leticia Rivero Gonzalez[1; Gabriel Lupon Escobar[2; LluisTil Perez[2,3; Sara Estevez Sarmiento[4

    2014-01-01

    Platelet-rich plasma (PRP) therapy in orthopedics and sports medicine has seen an expansion in use since 2003. Theobjective of this study is to not only review evidence of clinical effectiveness but also to evaluate its security and detect any disparitybetween its use and its popularity. A prospective, descriptive study, which included 104 athletes were performed. Minor adverse effectswere recorded and these were self-limited. Although there is a lack of more detailed studies which compare other factors, PRP therapyhas been shown to have positive results in relation to certain lesions.

  19. Combination of platelet rich fibrin, hydroxyapatite and PRF membrane in the management of large inflammatory periapical lesion

    Directory of Open Access Journals (Sweden)

    Vasundara Yayathi Shivashankar

    2013-01-01

    On the basis of the results obtained in our case report, we hypothesize that the use of PRF in conjunction with HA crystals might have accelerated the resorption of the graft crystals and would have induced the rapid rate of bone formation.

  20. Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel

    Directory of Open Access Journals (Sweden)

    Giraldo Carlos E

    2013-02-01

    Full Text Available Abstract Background There is no information on the effects of the breed, gender and age on the cellular content and growth factor (GF release from equine pure-platelet rich plasma (P-PRP and pure-platelet rich gel (P-PRG. The objectives of this study were: 1 to compare the cellular composition of P-PRP with whole blood and platelet poor plasma (PPP; 2 to compare the concentration of transforming GF beta 1 (TGF-β1 and platelet derived GF isoform BB (PDGF-BB between P-PRP treated with non-ionic detergent (P-PRP+NID, P-PRG (activated with calcium gluconate -CG-, PPP+NID, PPP gel (PPG, and plasma and; 3 to evaluate and to correlate the effect of the breed, gender and age on the cellular and GF concentration for each blood component. Forty adult horses, 20 Argentinean Creole Horses (ACH and, 20 Colombian Creole Horses (CCH were included. Data were analyzed by parametric (i.e.: t-test, one way ANOVA and non parametric (Kruskal-Wallis test, Wilcoxon test tests. Correlation analysis was also performed by using the Spearman and Pearson tests. A p ≤ 0.05 was set as significant for all tests. All the blood components were compared for platelet (PLT, leukocyte (WBC, TGF-β1 and PDGF-BB concentrations. The effect of the breed, gender and age on these variables was analyzed. A P ≤ 0.05 was accepted as significant for all the tests. Results PLT counts were 1.8 and 0.6 times higher in P-PRP than in whole blood and PPP, respectively; WBC counts were 0.5 and 0.1 times lower in P-PRP, in comparison with whole blood and PPP, respectively. TGF-β1 and PDGF-BB concentrations were 2.3 and 262 times higher, respectively, in P-PRG than in plasma, and 0.59 and 0.48 times higher, respectively, in P-PRG than in PPG. P-PRG derived from CCH females or young horses presented significantly (P Conclusions Our results indicated that P-PRP obtained by a manual method was affected by intrinsic factors such as the breed, gender and age. Equine practitioners should be

  1. Adipose-derived stem cells and platelet-rich plasma: the keys to functional periodontal tissue engineering.

    Science.gov (United States)

    Tobita, Morikuni; Mizuno, Hiroshi

    2013-09-01

    Numerous different types of periodontal tissue regeneration therapies have been developed clinically with variable outcomes and serious limitations. A key goal of periodontal therapy is to regenerate the destroyed periodontal tissues including alveolar bone, cementum and periodontal ligament. The critical factors in attaining successful periodontal tissue regeneration are the correct recruitment of cells to the site and the production of a suitable extra cellular matrix consistent with the periodontal tissues. Adipose tissue, from which mesenchymal stem cells can be harvested easily and safely, is an especially attractive stem cell source, because adipose-derived stem cells have a strong potential for cell differentiation and growth factor secretion. Meanwhile, the usefulness of platelet-rich plasma in the field of dental surgery has attracted attention. Therapeutic effects of platelet-rich plasma are believed to occur through the provision of concentrated levels of platelet-derived growth factors. Further, recent reports suggested the effect of platelet-rich plasma on mesenchymal stem cell proliferation, differentiation and survival rate. Therefore, the admixture of mesenchymal stem cells and platelet-rich plasma may indicate the great potential for tissue regenerations including periodontal tissue regeneration. In this review, the potential of adipose-derived stem cells and platelet-rich plasma is introduced. Of particular interest, the usefulness in periodontal tissue regeneration and future perspective is discussed.

  2. Biodegradable electrospun nanofibers coated with platelet-rich plasma for cell adhesion and proliferation

    Energy Technology Data Exchange (ETDEWEB)

    Diaz-Gomez, Luis [Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Santiago de Compostela, 15872 Santiago de Compostela (Spain); Instituto de Ortopedia y Banco de Tejidos Musculoesqueléticos, Universidad de Santiago de Compostela, 15872 Santiago de Compostela (Spain); Alvarez-Lorenzo, Carmen, E-mail: carmen.alvarez.lorenzo@usc.es [Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Santiago de Compostela, 15872 Santiago de Compostela (Spain); Concheiro, Angel [Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Santiago de Compostela, 15872 Santiago de Compostela (Spain); Silva, Maite [Instituto de Ortopedia y Banco de Tejidos Musculoesqueléticos, Universidad de Santiago de Compostela, 15872 Santiago de Compostela (Spain); Dominguez, Fernando [Fundación Publica Galega de Medicina Xenómica, Santiago de Compostela (Spain); Sheikh, Faheem A.; Cantu, Travis; Desai, Raj; Garcia, Vanessa L. [Department of Chemistry, University of Texas Pan American, Edinburg, TX 78541 (United States); Macossay, Javier, E-mail: jmacossay@utpa.edu [Department of Chemistry, University of Texas Pan American, Edinburg, TX 78541 (United States)

    2014-07-01

    Biodegradable electrospun poly(ε-caprolactone) (PCL) scaffolds were coated with platelet-rich plasma (PRP) to improve cell adhesion and proliferation. PRP was obtained from human buffy coat, and tested on human adipose-derived mesenchymal stem cells (MSCs) to confirm cell proliferation and cytocompatibility. Then, PRP was adsorbed on the PCL scaffolds via lyophilization, which resulted in a uniform sponge-like coating of 2.85 (S.D. 0.14) mg/mg. The scaffolds were evaluated regarding mechanical properties (Young's modulus, tensile stress and tensile strain), sustained release of total protein and growth factors (PDGF-BB, TGF-β1 and VEGF), and hemocompatibility. MSC seeded on the PRP–PCL nanofibers showed an increased adhesion and proliferation compared to pristine PCL fibers. Moreover, the adsorbed PRP enabled angiogenesis features observed as neovascularization in a chicken chorioallantoic membrane (CAM) model. Overall, these results suggest that PRP–PCL scaffolds hold promise for tissue regeneration applications. - Highlights: • Platelet-rich plasma (PRP) can be adsorbed on electrospun fibers via lyophilization. • PRP coating enhanced mesenchymal stem cell adhesion and proliferation on scaffolds. • PRP-coated scaffolds showed sustained release of growth factors. • Adsorbed PRP provided angiogenic features. • PRP-poly(ε-caprolactone) scaffolds hold promise for tissue regeneration applications.

  3. Platelet-rich Preparation may serve as a Powerful Tool for Therapeutic Dental Pulp Regeneration

    Directory of Open Access Journals (Sweden)

    Hai-Hua Sun

    2011-09-01

    Full Text Available Introduction: Regeneration of dental pulp tissues presents one of the most challenging issues in regenerative dentistry due to their extremely poor intrinsic ability for self-healing and re-growth.The hypothesis: We hypothes-ize that patient-derived platelet-rich preparation can be used in clinical endodontic regenerative procedure, serving as a powerful tool for therapeutic dental pulp regeneration.Evaluation of the hypothesis: The cell transplantation does not always obtain the good result because of the low survival rate of transplanted cells. In addition, the use of ex vivo manipulated cell products faces many translational hurdles in treating non-vital disease. Recently, the body cells are focused as a potential source for therapeutics. Some researchers have demonstrated that endogenous stem cells may be recruited to a desired anatomic site pharma-cologically. This is spurring interest in developing new generation of biomaterials that incorporate and release selected powerful extracellular influences in a near-physiological fashion, and subsequently capture endogenous stem cells and influence their fates for regene-ration. The use of patient-derived products such as platelet-rich preparations that contain a multitude of endogenous growth factors and proteins is a clinically translatable biotechnology for this proposes. These simple and cost efficient procedures may have a potential impact in reducing the economic costs for standard medical treatments in regenerative endodontics.

  4. Effect of Platelet-Rich Plasma on CCl4-Induced Chronic Liver Injury in Male Rats.

    Science.gov (United States)

    Hesami, Zahra; Jamshidzadeh, Akram; Ayatollahi, Maryam; Geramizadeh, Bita; Farshad, Omid; Vahdati, Akbar

    2014-01-01

    Platelet-rich plasma (PRP) has been of great concern to the scientists and doctors who are involved in wound healing and regenerative medicine which focuses on repairing and replacing damaged cells and tissues. Growth factors of platelet-rich plasma are cost-effective, available, and is more stable than recombinant human growth factors. Given these valuable properties, we decided to assess the effect of PRP on CCl4-induced hepatotoxicity on rats. The rats received CCl4 (1 mL/kg, i.p. 1 : 1 in olive oil) twice per week for 8 weeks. Five weeks after CCl4 injection, the rats also received PRP (0.5 mL/kg, s.c.) two days a week for three weeks. Twenty-four hours after last CCl4 injection, the animals bled and their livers dissected for biochemical and histopathological studies. Blood analysis was performed to evaluate enzyme activity. The results showed that PRP itself was not toxic for liver and could protect the liver from CCl4-induced histological damages and attenuated oxidative stress by increase in glutathione content and decrease in lipid peroxidative marker of liver tissue. The results of the present study lend support to our beliefs in hepatoprotective effects of PRP. PMID:24707405

  5. Biodegradable electrospun nanofibers coated with platelet-rich plasma for cell adhesion and proliferation

    International Nuclear Information System (INIS)

    Biodegradable electrospun poly(ε-caprolactone) (PCL) scaffolds were coated with platelet-rich plasma (PRP) to improve cell adhesion and proliferation. PRP was obtained from human buffy coat, and tested on human adipose-derived mesenchymal stem cells (MSCs) to confirm cell proliferation and cytocompatibility. Then, PRP was adsorbed on the PCL scaffolds via lyophilization, which resulted in a uniform sponge-like coating of 2.85 (S.D. 0.14) mg/mg. The scaffolds were evaluated regarding mechanical properties (Young's modulus, tensile stress and tensile strain), sustained release of total protein and growth factors (PDGF-BB, TGF-β1 and VEGF), and hemocompatibility. MSC seeded on the PRP–PCL nanofibers showed an increased adhesion and proliferation compared to pristine PCL fibers. Moreover, the adsorbed PRP enabled angiogenesis features observed as neovascularization in a chicken chorioallantoic membrane (CAM) model. Overall, these results suggest that PRP–PCL scaffolds hold promise for tissue regeneration applications. - Highlights: • Platelet-rich plasma (PRP) can be adsorbed on electrospun fibers via lyophilization. • PRP coating enhanced mesenchymal stem cell adhesion and proliferation on scaffolds. • PRP-coated scaffolds showed sustained release of growth factors. • Adsorbed PRP provided angiogenic features. • PRP-poly(ε-caprolactone) scaffolds hold promise for tissue regeneration applications

  6. Heparin-conjugated poly(lactic-co-glycolic acid) nanospheres enhance large-wound healing by delivering growth factors in platelet-rich plasma.

    Science.gov (United States)

    La, Wan-Geun; Yang, Hee Seok

    2015-04-01

    Platelet-rich plasma (PRP) contains many growth factors that are involved in tissue regeneration processes. For successful tissue regeneration, protein growth factors require a delivery vehicle for long-term and sustained release to a defect site in order to maintain their bioactivity. Previously, we showed that heparin-conjugated poly(lactic-co-glycolic acid) nanospheres (HCPNs) can provide long-term delivery of growth factors with affinity for heparin. In this study, we hypothesize that treatment of a skin wound with a mixture of PRP and HCPNs would provide long-term delivery of several growth factors contained in PRP to promote the skin wound healing process with preservation of bioactivity. The release of platelet-derived growth factor-BB (PDGF-BB), contained in PRP, from HCPN with fibrin gel (FG) showed a prolonged release period versus a PRP mixture with FG alone (FG-PRP). Also, growth factors released from PRP with HCPN and FG showed sustained human dermal fibroblast growth for 12 days. Full-thickness skin wound treatment in mice with FG-HCPN-PRP resulted in much faster wound closure as well as dermal and epidermal regeneration at day 9 compared with treatment with FG-HCPN or FG-PRP. The enhanced wound healing using FG-HCPN-PRP may be due to the prolonged release not only of PDGF-BB but also of other growth factors in the PRP. The delivered growth factors accelerated angiogenesis at the wound site.

  7. Shoulder surgeon and autologous cellular regeneration - From bench to bed: Part one- the link between the human fibroblast, connective tissue disorders and shoulder

    Directory of Open Access Journals (Sweden)

    Du Toit Don

    2007-01-01

    Full Text Available The process of autologous cell regeneration (ACR is a facet of cell therapy and regenerative medicine. It is initiated when activated autologous platelet rich plasma alone (PRP, containing cytokines or growth factors, is injected into the dermis or other structure in order to initiate a regenerative or antiaging process. The recipients resident cells at the target zone are activated by biologically active growth factors, derived from the activated platelets in the PRP through a paracrine effect. The platelet gel that contains the fibrin and clumped platelets, releases growth factors that influences activation of macrophages and stem cells in the recipient site. Tissue regeneration is facilitated by stem cell proliferation and differentiation. The PRP that has sealant and wound healing properties, may shift the wound healing cascade to the left, thereby speeding up tissue regeneration and remodelling by the use of the recipients own plasma. Both the fibroblast and myofibroblast play key roles in the wound healing cascade. The fibroblast, of mesenchymal origin, plays a pivotal role in the formation of the extracellular matrix and deposition of collagen. A failure of organised fibroblast function results in important and disabling disease processes and conditions such as chronic ischaemic heart disease and remodelling of the heart, lung fibrosis, fibromatosis, solar aged-face, keloids, hypertrophic scar formation, nodular fasciitis, inguinal hernia, Dupuytren′s disease and scleroderma. More recent additions include frozen shoulder and the captured shoulder. In aesthetic medicine, one of the prime functions of fractional photothermolysis with lasers, intense pulsed light and radiofrequency devices, is to stimulate dermal fibroblast proliferation, by thermal energy thereby increasing collagen deposition which enhances facial rejuvenation. Platelet -rich plasma (REGENLAB PRP, REGENLAB-ACR possesses unique growth factors that stimulate, fibroblast

  8. Platelet-Rich-Plasma injection seems to be effective in treatment of plantar fasciitis: a case series.

    Science.gov (United States)

    van Egmond, Jeroen C; Breugem, Stefan J M; Driessen, Marcel; Bruijn, Daniel J

    2015-06-01

    Plantar fasciitis is the most common cause of heel pain. Diverse non-operative treatment options are available. The purpose of this study was to determine if a single platelet-rich-plasma injection at the origin of the plantar fascia in patients with plantar fasciitis gives a functional improvement. Patients with plantar fasciitis and failed conservative treatment were included in this retrospective study. Included patients were sent four questionnaires after platelet-rich-plasma injection. Primary outcome is functional improvement, determined by foot function index in which lower scores correlates with a better foot function. A total of 61 feet in 58 patients were included. The median foot function index before treatment was 69.4 and after treatment 31.8, which is a significant decrease. In 80.3% of the patients the foot function index decreased. Therefore platelet-rich-plasma injection seems to be effective in treatment of patients with plantar fasciitis when conservative treatment failed. PMID:26280973

  9. Comparison between the effects of platelet-rich plasma and bone marrow concentrate on defect consolidation in the rabbit tibia

    OpenAIRE

    Batista, Marco Antonio; Leivas, Tomaz Puga; Rodrigues, Consuelo Junqueira; Arenas, Géssica Cantadori Funes; Belitardo, Donizeti Rodrigues; Guarniero, Roberto

    2011-01-01

    OBJECTIVE: To perform a comparative analysis of the effects of platelet-rich plasma and centrifuged bone marrow aspirate on the induction of bone healing in rabbits. METHOD: Twenty adult, male New Zealand rabbits were randomly separated into two equal groups, and surgery was performed to create a bone defect (a cortical orifice 3.3 mm in diameter) in the proximal metaphysis of each rabbit's right tibia. In the first group, platelet-rich plasma was implanted in combination with β-tricalcium ph...

  10. Safety and efficient ex vivo expansion of stem cells using platelet-rich plasma technology.

    Science.gov (United States)

    Anitua, Eduardo; Prado, Roberto; Orive, Gorka

    2013-09-01

    The goal of this Review is to provide an overview of the cell culture media supplements used in the ex vivo expansion of stem cells intended for cell therapy. Currently, the gold standard is the culture supplemented with fetal bovine serum, however, their use in cell therapy raises many concerns. The alternatives to its use are presented, ranging from the use of human serum to platelet-rich plasma (PRP), to serum-free media or extracellular matrix components. Finally, various growth factors present in PRP are described, which make it a safe and effective stem cell expansion supplement. These growth factors could be responsible for their efficiency, as they increase both stem cell proliferation and survival. The different PRP formulations are also discussed, as well as the need for protocol standardization.

  11. Ultrasound-Guided Injection Therapy of Achilles Tendinopathy With Platelet-Rich Plasma or Saline

    DEFF Research Database (Denmark)

    Krogh, Thøger P; Ellingsen, Torkell; Jensen, Pia;

    2016-01-01

    BACKGROUND: Achilles tendinopathy (AT) is a common and difficult to treat musculoskeletal disorder. PURPOSE: To examine whether 1 injection of platelet-rich plasma (PRP) would improve outcomes more effectively than placebo (saline) after 3 months in patients with AT. STUDY DESIGN: Randomized...... controlled trial; Level of evidence, 1. METHODS: A total of 24 patients with chronic AT (median disease duration, 33 months) were randomized (1:1) to receive either a blinded injection of PRP (n = 12) or saline (n = 12). The primary endpoint was improvement in Victorian Institute of Sports Assessment....... RESULTS: After 3 months, all 24 patients were reassessed (no dropouts). No difference between the PRP and the saline group could be observed with regard to the primary outcome (VISA-A score: mean difference [MD], -1.3; 95% CI, -17.8 to 15.2; P = .868). Secondary outcomes were pain at rest (MD, 1.6; 95% CI...

  12. European definitions, current use, and EMA stance of platelet-rich plasma in sports medicine.

    Science.gov (United States)

    Fiorentino, Stefano; Roffi, Alice; Filardo, Giuseppe; Marcacci, Maurilio; Kon, Elizaveta

    2015-02-01

    Platelet-rich plasma has been the focus of much attention over the last few years as an appealing biological approach to favor the healing of tissues otherwise doomed by a low healing potential. In Europe, the regulatory framework concerning the blood system is currently disciplined by Directive 2002/98/EC of the European Parliament and Council of January 27, 2003, which sets out quality and safety rules for collecting, controlling, processing, preserving, and distributing human blood and its components, acknowledged in the various States of the Union with internal regulations. This lack of homogeneity in the European legal landscape will probably lead the Community legislature to intervene in the near future, to even out the "rules of engagement" of this peculiar class of biomaterials.

  13. Classical scrapie prions in ovine blood are associated with B lymphocytes and platelet-rich plasma

    Directory of Open Access Journals (Sweden)

    Dassanayake Rohana P

    2011-11-01

    Full Text Available Abstract Background Classical scrapie is a naturally occurring transmissible spongiform encephalopathy of sheep and goats characterized by cellular accumulation of abnormal isoforms of prion protein (PrPSc in the central nervous system and the follicles of peripheral lymphoid tissues. Previous studies have shown that the whole blood and buffy coat blood fraction of scrapie infected sheep harbor prion infectivity. Although PrPSc has been detected in peripheral blood mononuclear cells (PBMCs, plasma, and more recently within a subpopulation of B lymphocytes, the infectivity status of these cells and plasma in sheep remains unknown. Therefore, the objective of this study was to determine whether circulating PBMCs, B lymphocytes and platelets from classical scrapie infected sheep harbor prion infectivity using a sheep bioassay. Results Serial rectal mucosal biopsy and immunohistochemistry were used to detect preclinical infection in lambs transfused with whole blood or blood cell fractions from preclinical or clinical scrapie infected sheep. PrPSc immunolabeling was detected in antemortem rectal and postmortem lymphoid tissues from recipient lambs receiving PBMCs (15/15, CD72+ B lymphocytes (3/3, CD21+ B lymphocytes (3/3 or platelet-rich plasma (2/3 fractions. As expected, whole blood (11/13 and buffy coat (5/5 recipients showed positive PrPSc labeling in lymphoid follicles. However, at 549 days post-transfusion, PrPSc was not detected in rectal or other lymphoid tissues in three sheep receiving platelet-poor plasma fraction. Conclusions Prion infectivity was detected in circulating PBMCs, CD72+ pan B lymphocytes, the CD21+ subpopulation of B lymphocytes and platelet-rich plasma of classical scrapie infected sheep using a sheep bioassay. Combining platelets with B lymphocytes might enhance PrPSc detection levels in blood samples.

  14. Effects of platelet-rich plasma, adipose-derived stem cells, and stromal vascular fraction on the survival of human transplanted adipose tissue.

    Science.gov (United States)

    Kim, Deok-Yeol; Ji, Yi-Hwa; Kim, Deok-Woo; Dhong, Eun-Sang; Yoon, Eul-Sik

    2014-11-01

    Traditional adipose tissue transplantation has unpredictable viability and poor absorption rates. Recent studies have reported that treatment with platelet-rich plasma (PRP), adipose-derived stem cells (ASCs), and stromal vascular fraction (SVF) are related to increased survival of grafted adipose tissue. This study was the first simultaneous comparison of graft survival in combination with PRP, ASCs, and SVF. Adipose tissues were mixed with each other, injected subcutaneously into the back of nude mice, and evaluated at 4, 8, and 12 weeks. Human adipocytes were grossly maintained in the ASCs and SVF mixtures. Survival of the adipose tissues with PRP was observed at 4 weeks and with SVF at 8 and 12 weeks. At 12 weeks, volume reduction in the ASCs and SVF mixtures were 36.9% and 32.1%, respectively, which were significantly different from that of the control group without adjuvant treatment, 51.0%. Neovascular structures were rarely observed in any of the groups. Our results suggest that the technique of adding ASCs or SVF to transplanted adipose tissue might be more effective than the conventional grafting method. An autologous adipose tissue graft in combination with ASCs or SVF may potentially contribute to stabilization of engraftment.

  15. Effect of platelet-rich plasma on the healing of mandibular defects treated with fresh frozen bone allograft: a radiographic study in dogs.

    Science.gov (United States)

    Messora, Michel R; Nagata, Maria J H; Fucini, Stephen E; Pola, Natália M; Campos, Natália; de Oliveira, Guillermo C V; Bosco, Alvaro F; Garcia, Valdir G; Furlaneto, Flávia A C

    2014-10-01

    The purpose of this study was to radiographically analyze the effect of autologous platelet rich plasma (PRP) on the healing of fresh frozen bone allograft (FFBA) placed in surgically created resection defects in mandibles of dogs. Bilateral resection defects measuring 1.5 cm × 1 cm were surgically created on the inferior border of the mandible in 10 adult male dogs. The defects were randomly divided into three groups: C, FFBA, and FFBA/PRP. In Group C, the defect was filled by blood clot only. In Group FFBA, the defect was filled with particulate fresh frozen bone allograft. In Group FFBA/PRP, it was filled with particulate fresh frozen bone allograft combined with PRP. At 90 days postoperative, standardized radiographs of the mandibles were obtained and results were quantitatively evaluated. Analysis of digitized radiographs indicated that non-PRP grafts were significantly less dense than the PRP grafts. Group FFBA/PRP also presented a statistically greater mineralized tissue area than Groups C and FFBA. Within the limits of this study, it can be concluded that PRP enhanced the healing of FFBA in resection defects in mandibles of dogs.

  16. An exercise-based physical therapy program for patients with patellar tendinopathy after platelet-rich plasma injection

    NARCIS (Netherlands)

    van Ark, Mathijs; van den Akker-Scheek, Inge; Meijer, L.T.B.; Zwerver, Hans

    2013-01-01

    Objectives: To describe a post platelet-rich plasma (PRP) injection, exercise-based physical therapy program, investigate feasibility and report the first results of patellar tendinopathy patients treated with PRP injection combined with the physical therapy program. Study Design: Case-series. Setti

  17. Symptomatic knee osteoarthritis treatment using autologous adipose derived stem cells and platelet-rich plasma: a clinical study

    OpenAIRE

    Phuc Van Pham; Khanh Hong-Thien Bui; Triet Dinh Duong; Nhan Thanh Nguyen; Thanh Duc Nguyen; Vien Tuong Le; Viet Thanh Mai; Nhan Lu-Chinh Phan; Dung Minh Le and Ngoc Kim Ngoc

    2014-01-01

    Osteoarthritis is one of the most common diseases, and it affects 12% of the population around the world. Although the disease is chronic, it significantly reduces the patient's quality of life. At present, stem cell therapy is considered to be an efficient approach for treating this condition. Mesenchymal stem cells (MSCs) show the most potential for stem cell therapy of osteoarthritis. In fact, MSCs can differentiate into certain mesodermal tissues such as cartilage and bone. Therefore, in ...

  18. Transplantation of Autologous Bone Marrow Mesenchymal Stem Cells with Platelet-Rich Plasma Accelerate Distraction Osteogenesis in A Canine Model

    OpenAIRE

    2015-01-01

    Objective: Distraction osteogenesis (DO) is a surgical procedure used to generate large volumes of new bone for limb lengthening. Materials and Methods: In this animal experimental study, a 30% lengthening of the left tibia (mean distraction distance: 60.8 mm) was performed in ten adult male dogs by callus distraction after osteotomy and application of an Ilizarov fixator. Distraction was started on postoperative day seven with a distraction rate of 0.5 mm twice per day and car...

  19. Autologous platelet-rich plasma compared with whole blood for the treatment of chronic plantar fasciitis; a comparative clinical trial

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    Babak Vahdatpour

    2016-01-01

    Conclusions: Significant improvement in pain and function, as well as decrease in plantar fascia thickness, was observed by intralesional injection of the PRP and WB in patients with chronic PF. The study results indicate similar effectiveness between PRP and WB for the treatment of chronic PF in short-term.

  20. The effects of amlodipine and platelet rich plasma on bone healing in rats

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    Atalay Y

    2015-04-01

    Full Text Available Yusuf Atalay,1 Mehmet Fatih Bozkurt,2 Yucel Gonul,3 Omer Cakmak,4 Kamil Serkan Agacayak,5 Ibrahim Köse,6 Omer Hazman,7 Hikmet Keles,2 Ozan Turamanlar,3 Mehmet Eroglu8 1Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Afyon Kocatepe University, Afyonkarahisar, Turkey; 2Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey; 3Department of Anatomy, Faculty of Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey; 4Department of Periodontology, Faculty of Dentistry, Afyon Kocatepe University, Afyonkarahisar, Turkey; 5Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey; 6Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Adiyaman University, Adiyaman, Turkey; 7Department of Biochemistry, Faculty of Sciences, Afyon Kocatepe University, Afyonkarahisar, Turkey; 8Department of Orthopedics and Traumatology, Faculty of Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey Aim: The aim of this study was to evaluate the effects of calcium channel blocker (CCB amlodipine (AML, platelet rich plasma (PRP, and a mixture of both materials on bone healing.Materials and methods: Fifty-six male Wistar rats were randomly divided into four groups: group A, tibia defect model with no treatment; group B, tibia defect model treated with AML, 0.04 mg daily by oral gavage; group C, tibia defect model treated with local PRP; group D, tibia defect model treated with local PRP and AML, 0.04 mg daily by oral gavage.Results: At day 21, bone healing was significantly better in groups C and D compared to group A (P<0.05, but comparisons showed no statistically significant difference in group B (P>0.05. At day 30, groups B and C showed no statistically significant difference (P>0.05 compared to group A, but bone healing in group D was significantly better than in group A (P<0.05. Statistically, AML did not affect alkaline

  1. An inside-out vein graft iflled with platelet-rich plasma for repair of a short sciatic nerve defect in rats

    Institute of Scientific and Technical Information of China (English)

    Ji Yeong Kim; Woo Joo Jeon; Dong Hwee Kim; Im Joo Rhyu; Young Hwan Kim; Inchan Youn; Jong Woong Park

    2014-01-01

    Platelet-rich plasma containing various growth factors can promote nerve regeneration. An in-side-out vein graft can substitute nerve autograft to repair short nerve defects. It is hypothesized that an inside-out vein graft iflled with platelet-rich plasma shows better effects in the repair of short sciatic nerve defects. In this study, an inside-out vein autograft iflled with platelet-rich plasma was used to bridge a 10 mm-long sciatic nerve defect in rats. The sciatic nerve function of rats with an inside-out vein autograft iflled with platelet-rich plasma was better improved than that of rats with a simple inside-out vein autograft. At 6 and 8 weeks, the sciatic nerve function of rats with an inside-out vein autograft filled with platelet-rich plasma was better than that of rats undergoing nerve autografting. Compared with the sciatic nerve repaired with a simple inside-out vein autograft, the number of myelinated axons was higher, axon diameter and myelin sheath were greater in the sciatic nerve repaired with an inside-out vein autograft iflled with platelet-rich plasma and they were similar to those in the sciatic nerve repaired with nerve autograft. These findings suggest that an inside-out vein graft filled with platelet-rich plasma can substitute nerve autograft to repair short sciatic nerve defects.

  2. Mechanisms of ascorbyl radical formation in human platelet-rich plasma.

    Science.gov (United States)

    Shyu, Kou-Gi; Chang, Chao-Chien; Yeh, Yu-Chieh; Sheu, Joen-Rong; Chou, Duen-Suey

    2014-01-01

    Recently, many clinical reports have suggested that the ascorbyl free radical (Asc(∙)) can be treated as a noninvasive, reliable, real-time marker of oxidative stress, but its generation mechanisms in human blood have rarely been discussed. In this study, we used upstream substances, enzyme inhibitors, and free radical scavengers to delineate the mechanisms of Asc(∙) formation in human platelet-rich plasma (PRP). Our results show that the doublet signal was detected in PRP samples by using electron spin resonance, and the hyperfine splitting of the doublet signal was a(H) = 1.88 gauss and g-factor = 2.00627, which was determined to be the Asc(∙). We observed that the inhibitors of NADPH oxidase (NOX), cyclooxygenase (COX), lipoxygenase (LOX), cytochrome P450 (CYP450), mitochondria complex III, and nitric oxide synthase (NOS), but not xanthine oxidase, diminished the intensity of the Asc(∙) signal dose dependently. All enzyme inhibitors showed no obvious antioxidant activity during a Fenton reaction assay. In summary, the obtained data suggest that Asc(∙) formation is associated with NOX, COX, LOX, CYP450, eNOS, and mitochondria in human PRP. PMID:24696859

  3. Mechanisms of Ascorbyl Radical Formation in Human Platelet-Rich Plasma

    Directory of Open Access Journals (Sweden)

    Kou-Gi Shyu

    2014-01-01

    Full Text Available Recently, many clinical reports have suggested that the ascorbyl free radical (Asc∙ can be treated as a noninvasive, reliable, real-time marker of oxidative stress, but its generation mechanisms in human blood have rarely been discussed. In this study, we used upstream substances, enzyme inhibitors, and free radical scavengers to delineate the mechanisms of Asc∙ formation in human platelet-rich plasma (PRP. Our results show that the doublet signal was detected in PRP samples by using electron spin resonance, and the hyperfine splitting of the doublet signal was aH=1.88 gauss and g-factor = 2.00627, which was determined to be the Asc∙. We observed that the inhibitors of NADPH oxidase (NOX, cyclooxygenase (COX, lipoxygenase (LOX, cytochrome P450 (CYP450, mitochondria complex III, and nitric oxide synthase (NOS, but not xanthine oxidase, diminished the intensity of the Asc∙ signal dose dependently. All enzyme inhibitors showed no obvious antioxidant activity during a Fenton reaction assay. In summary, the obtained data suggest that Asc∙ formation is associated with NOX, COX, LOX, CYP450, eNOS, and mitochondria in human PRP.

  4. Use of platelet-rich plasma in deep second- and third-degree burns.

    Science.gov (United States)

    Venter, Neil Grant; Marques, Ruy Garcia; Santos, Jeanine Salles Dos; Monte-Alto-Costa, Andréa

    2016-06-01

    Unfortunately burns are a common occurrence, leading to scarring or death. Platelet-rich plasma (PRP) contains many growth factors that can accelerate wound healing. We analyzed the use of PRP in deep second-degree (dSD), deep second-degree associated with diabetes mellitus (dSDD), and third-degree (TD) burns in rats. Sixty syngeneic rats divided into three groups (dSD, dSDD, and TD) were burned, half receiving topical PRP and half being used as control; 10 additional rats per group were used for PRP preparation. On day 21, the animals were sacrificed and skin biopsies were collected. dSD and dSDD wounds treated with PRP showed faster wound closure, reduction in CD31-, CD68-, CD163-, MPO-, and in TGF-β-positive cells, and an increase in MMP2-positive cells. The neo-epidermis was thinner in the control of both the dSD and dSDD groups and granulation tissue was less reduced in the control of both the dSDD and TD groups. These results indicate that PRP can accelerate the healing process in dSD and dSDD, but not in TD burns. PMID:26822695

  5. Platelet Concentration in Platelet-Rich Plasma Affects Tenocyte Behavior In Vitro

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    Ilaria Giusti

    2014-01-01

    Full Text Available Since tendon injuries and tendinopathy are a growing problem, sometimes requiring surgery, new strategies that improve conservative therapies are needed. Platelet-rich plasma (PRP seems to be a good candidate by virtue of its high content of growth factors, most of which are involved in tendon healing. This study aimed to evaluate if different concentrations of platelets in PRP have different effects on the biological features of normal human tenocytes that are usually required during tendon healing. The different platelet concentrations tested (up to 5 × 106 plt/µL stimulated differently tenocytes behavior; intermediate concentrations (0.5 × 106, 1 × 106 plt/µL strongly induced all tested processes (proliferation, migration, collagen, and MMPs production if compared to untreated cells; on the contrary, the highest concentration had inhibitory effects on proliferation and strongly reduced migration abilities and overall collagen production but, at the same time, induced increasing MMP production, which could be counterproductive because excessive proteolysis could impair tendon mechanical stability. Thus, these in vitro data strongly suggest the need for a compromise between extremely high and low platelet concentrations to obtain an optimal global effect when inducing in vivo tendon healing.

  6. Effects of platelet-rich plasma on lateral epicondylitis of the elbow: prospective randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Evandro Pereira Palacio

    2016-02-01

    Full Text Available ABSTRACT OBJECTIVE: To evaluate the effects of platelet-rich plasma (PRP infiltration in patients with lateral epicondylitis of the elbow, through analysis of the Disabilities of the Arm, Shoulder and Hand (DASH and Patient-Rated Tennis Elbow Evaluation (PRTEE questionnaires. METHODS: Sixty patients with lateral epicondylitis of the elbow were prospectively randomized and evaluated after receiving infiltration of three milliliters of PRP, or 0.5% neocaine, or dexamethasone. For the scoring process, the patients were asked to fill out the DASH and PRTEE questionnaires on three occasions: on the day of infiltration and 90 and 180 days afterwards. RESULTS: Around 81.7% of the patients who underwent the treatment presented some improvement of the symptoms. The statistical tests showed that there was evidence that the cure rate was unrelated to the substance applied (p = 0.62. There was also intersection between the confidence intervals of each group, thus demonstrating that the proportions of patients whose symptoms improved were similar in all the groups. CONCLUSION: At a significance level of 5%, there was no evidence that one treatment was more effective than another, when assessed using the DASH and PRTEE questionnaires.

  7. Assessment of Vascular Endothelial Growth Factor in Fresh versus Frozen Platelet Rich Plasma.

    Science.gov (United States)

    Hosny, Nada; Goubran, Fikry; BadrEldin Hasan, Basma; Kamel, Noha

    2015-01-01

    Platelet rich plasma (PRP) is hemoconcentration with platelets concentration above baseline values and high concentration of many growth factors. The aim of this study was to assess freezing effect on vascular endothelial growth factor (VEGF) release from PRP using two different activation methods to simplify its use in different clinical applications. PRP was prepared using two-centrifugation steps method from 12 qualified blood donors. VEGF concentrations were measured in fresh PRP and after freezing/thawing for one and three weeks with two methods of activation using (i) calcium gluconate and (ii) calcium gluconate and thrombin. Platelets count was significantly increased compared to baseline whole blood values in all fresh and frozen PRP samples (p value was VEGF concentrations after activating fresh and frozen-thawed PRP samples for one and three weeks by calcium alone or calcium with thrombin, and also no significant difference was found when freezing period was extended from one to three weeks. Our results showed that platelets count does not correlate with variable levels of VEGF. PRP could be prepared once and preserved frozen for at least three weeks for the next treatment sessions and activation with thrombin addition to calcium will not augment the growth factor release. PMID:26301115

  8. Assessment of Vascular Endothelial Growth Factor in Fresh versus Frozen Platelet Rich Plasma

    Directory of Open Access Journals (Sweden)

    Nada Hosny

    2015-01-01

    Full Text Available Platelet rich plasma (PRP is hemoconcentration with platelets concentration above baseline values and high concentration of many growth factors. The aim of this study was to assess freezing effect on vascular endothelial growth factor (VEGF release from PRP using two different activation methods to simplify its use in different clinical applications. PRP was prepared using two-centrifugation steps method from 12 qualified blood donors. VEGF concentrations were measured in fresh PRP and after freezing/thawing for one and three weeks with two methods of activation using (i calcium gluconate and (ii calcium gluconate and thrombin. Platelets count was significantly increased compared to baseline whole blood values in all fresh and frozen PRP samples (p value was <0.05. No significant difference was found between VEGF concentrations after activating fresh and frozen-thawed PRP samples for one and three weeks by calcium alone or calcium with thrombin, and also no significant difference was found when freezing period was extended from one to three weeks. Our results showed that platelets count does not correlate with variable levels of VEGF. PRP could be prepared once and preserved frozen for at least three weeks for the next treatment sessions and activation with thrombin addition to calcium will not augment the growth factor release.

  9. Platelet-rich plasma in androgenic alopecia: Myth or an effective tool

    Directory of Open Access Journals (Sweden)

    Swapna S Khatu

    2014-01-01

    Full Text Available Platelet-rich plasma (PRP has become a newer method for the treatment of various types of alopecia. In this prospective study, safety, efficacy and feasibility of PRP injections in treating androgenic alopecia were assessed. Eleven patients suffering from hair loss due to androgenic alopecia and not responding to 6 months treatment with minoxidil and finasteride were included in this study. The hair pull test was performed before every treatment session. A total volume of 2-3 cc PRP was injected in the scalp by using an insulin syringe. The treatment was repeated every two weeks, for a total of four times. The outcome was assessed after 3 months by clinical examination, macroscopic photos, hair pull test and patient′s overall satisfaction. Results: A significant reduction in hair loss was observed between first and fourth injection. Hair count increased from average number of 71 hair follicular units to 93 hair follicular units. Therefore, average mean gain is 22.09 follicular units per cm 2. After the fourth session, the pull test was negative in 9 patients. Conclusion: PRP injection is a simple, cost effective and feasible treatment option for androgenic alopecia, with high overall patient satisfaction.

  10. Bilateral quadriceps rupture: results with and without platelet-rich plasma.

    Science.gov (United States)

    Lanzetti, Riccardo Maria; Vadalà, Antonio; Morelli, Federico; Iorio, Raffaele; Ciompi, Alessandro; Vetrano, Mario; Argento, Giuseppe; Vulpiani, Maria Chiara; Di Sanzo, Vincenzo; Ferretti, Andrea

    2013-11-01

    This article presents a 46-year-old man with bilateral atraumatic quadriceps rupture that occurred while he was descending stairs. The patient underwent surgery the day after the accident. In the left knee, quadriceps reinsertion was performed using a conventional technique. In the right knee, platelet-rich plasma (PRP), both in its liquid and semisolid patterns, was added intraoperatively. Ultrasonography and magnetic resonance imaging evaluations were performed 1, 6, and 24 months postoperatively. At 6 and 24 months postoperatively, clinical and functional evaluations also were performed. Clinical examination showed no differences between the knees, and functional scores were the same for both knees. Ultrasonographic evaluation showed bilateral persistent tendon thickening and gross echotexture abnormalities, with no side-to-side differences. Magnetic resonance imaging showed signals of vascularized granulation tissue in both knees, which was more evident in the right (PRP) knee at 1 month postoperatively, along with a better signal of scar tissue in the right knee at 6 and 24 months postoperatively. The use of PRP yielded no better clinical or functional results than the lack of its use. However, a more intense and significant reparative healing process occurred where the PRP was used, thus suggesting a more rapid completion of the healing process, although this effect seems to remain only a radiographic finding with no clinical correlation.

  11. Platelet rich plasma associated with heterologous fresh and thawed chondrocytes on osteochondral lesions of rabbits

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    R.R. Filgueiras

    2014-02-01

    Full Text Available Chondrocytes obtained from stifle joint of New Zealand White rabbits were cultivated. Half of cells were maintained in culture for later implantation and the others frozen during six months to evaluate viability. A circular osteochondral defect was created in the right stifle of other twenty seven rabbits. The control group (CG received no treatment. The thawed (TH and fresh (FH heterologous groups received, respectively, an implant of cultivated thawed or fresh heterologous chondrocytes associated with platelet rich plasma (PRP. The CG group showed greatest pain and lameness compared to the other groups seven days after the implantation. Microscopically, at 45 and 90 days, the TH and FH groups showed filling with cartilaginous tissue containing chondrocytes surrounded by a dense matrix of glycosaminoglycans. In the CG group, healing occurred with vascularized fibrous connective tissue without integration to the subchondral bone. Cryopreserved heterologous chondrocytes were viable for implantation and healing of osteochondral lesions; the association with PRP allows the fixation of cells in the lesion and offers growth factors which accelerates repair with tissue similar to articular hyaline cartilage.

  12. Role of platelet-rich plasma in chronic alopecia areata: Our centre experience

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    Sukhbir Singh

    2015-01-01

    Full Text Available Aim: The aim of the study was to evaluate the efficacy of platelet-rich plasma (PRP in the treatment of chronic alopecia areata (AA. Material and Methods: This is a prospective study that was conducted at Kamal Hospital, Kaushambi in which 20 patients who attended the outpatient department were enrolled for the study. All the patients had h/o patches and taken various line of treatments for a duration of 2 years. All the patients were biopsy-proven positive for AA disease. There was no randomisation done since all of them were healthy young adults. The patients′age ranged from 25 to 35 years, and none of them had any co-morbidities. Results: Of 20 patients, only one patient had a relapse. None of the patients had any side effects, and all of them tolerated the procedure well. Conclusion: We wish to conclude that PRP has a definite role in treating AA infections. However, still more long-term follow-up, studies are required for further validation of results and labelling it as a 100% cure for people suffering from AA with recurrences which are so common.

  13. Diabetic foot ulcer treatment by activated platelet rich plasma: a clinical study

    Directory of Open Access Journals (Sweden)

    Tung Dang-Xuan Tran

    2014-02-01

    Full Text Available Diabetic foot ulcer is a major complication of diabetes mellitus. It occurred in about 15% of all diabetic patients. To date, the outcome of management of diabetic foot ulcer is poor and low sufficient. Some new therapies were suggested to manage and treat this disease. In almost therapies, management of diabetic foot ulcer relates to debridement of the wound, revascularization, off-loading of the ulcer, antibacterial actions, stimulating granulation, epidermization and angiogenesis. This study aimed to evaluate the effects of activated platelet rich plasma (aPRP on diabetic foot ulcer healing on volunteer patients. There were 6 patients enrolled in this study. All patients have non-healing foot ulcers. aPRP was isolated from peripheral blood and activated with calcium chloride. Patients were injected with aPRP two times with 14-day interval. All patients were monitored during 12 weeks. The results showed that 100% (6/6 ulcers completely closed after about 7 weeks. This result initially suggests that aPRP injection is efficient method to treat the non-healing foot ulcers. Level of evidence: IV [Biomed Res Ther 2014; 1(2.000: 37-42

  14. Effects of platelet-rich plasma on lateral epicondylitis of the elbow: prospective randomized controlled trial☆

    Science.gov (United States)

    Palacio, Evandro Pereira; Schiavetti, Rafael Ramos; Kanematsu, Maiara; Ikeda, Tiago Moreno; Mizobuchi, Roberto Ryuiti; Galbiatti, José Antônio

    2016-01-01

    Objective To evaluate the effects of platelet-rich plasma (PRP) infiltration in patients with lateral epicondylitis of the elbow, through analysis of the Disabilities of the Arm, Shoulder and Hand (DASH) and Patient-Rated Tennis Elbow Evaluation (PRTEE) questionnaires. Methods Sixty patients with lateral epicondylitis of the elbow were prospectively randomized and evaluated after receiving infiltration of three milliliters of PRP, or 0.5% neocaine, or dexamethasone. For the scoring process, the patients were asked to fill out the DASH and PRTEE questionnaires on three occasions: on the day of infiltration and 90 and 180 days afterwards. Results Around 81.7% of the patients who underwent the treatment presented some improvement of the symptoms. The statistical tests showed that there was evidence that the cure rate was unrelated to the substance applied (p = 0.62). There was also intersection between the confidence intervals of each group, thus demonstrating that the proportions of patients whose symptoms improved were similar in all the groups. Conclusion At a significance level of 5%, there was no evidence that one treatment was more effective than another, when assessed using the DASH and PRTEE questionnaires. PMID:26962506

  15. Histological, Biomechanical and Radiological Evaluation of Bone Repair with Human Platelet Rich Plasma in Rabbit Model

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    Zahra Shafiei-Sarvestani

    2015-02-01

    Full Text Available Background: This study was carried out to evaluation the effect of human platelet rich plasma (hPRP on the bone repair process in rabbit model which could be used in many procedures of orthopedic or maxillofacial bone and implant reconstructive surgery. Materials and Methods: This study is a prospective experimental study on animal model. A critical size defect (10 mm was created in the radial diaphysis of 24 rabbit and then supplied with human PRP (treatment group or the defect left empty (control group. Radiographs of each forelimb was taken postoperatively on 1st day and at the 2nd, 4th, 6th and 8th weeks post injury to evaluate bone formation, union and remodeling of the defect. The operated radii were removed on 56th postoperative day and were evaluated for biomechanical properties and histopathological criteria. Results: The results indicate that human PRP (as a xenogenic PRP in treatment group significantly promote bone regeneration in critical size defects compared with control group (p<0.05. Conclusion: This study showed that hPRP has a high regenerative capacity in critical size bone defects in rabbit model after 8 weeks.

  16. Leukocyte-Reduced Platelet-Rich Plasma Treatment of Basal Thumb Arthritis: A Pilot Study

    Science.gov (United States)

    Lang, Siegmund; Dendl, Lena-Marie; Nerlich, Michael; Angele, Peter; Gehmert, Sebastian

    2016-01-01

    A positive effect of intra-articular platelet-rich plasma (PRP) injection has been discussed for osteoarthritic joint conditions in the last years. The purpose of this study was to evaluate PRP injection into the trapeziometacarpal (TMC) joint. We report about ten patients with TMC joint osteoarthritis (OA) that were treated with 2 intra-articular PRP injections 4 weeks apart. PRP was produced using the Double Syringe System (Arthrex Inc., Naples, Florida, USA). A total volume of 1.47 ± 0.25 mL PRP was injected at the first injection and 1.5 ± 0.41 mL at the second injection, depending on the volume capacity of the joint. Patients were evaluated using VAS, strength measures, and the Mayo Wrist score and DASH score after 3 and 6 months. VAS significantly decreased from 6.2 ± 1.6 to 5.4 ± 2.2 at six-month follow-up (P TMC OA is a reasonable therapeutic option in early stages TMC OA and can be performed with little to no morbidity. PMID:27478842

  17. Plasma rico em plaquetas e fatores de crescimento: técnica de preparo e utilização em cirurgia plástica Platelet-rich plasma and growth factors: processing technique and application in plastic surgery

    Directory of Open Access Journals (Sweden)

    Fabiel Spani Vendramin

    2006-02-01

    Full Text Available OBJETIVO: Estabelecer um método barato e eficiente de preparação do plasma rico em plaquetas e fatores de crescimento para utilização em cirurgia plástica. MÉTODO: Foram realizados 20 testes através de centrifugação de sangue, variando-se a força e o tempo de centrifugação, para determinarmos o melhor método que proporcione uma maior concentração plaquetária e mais 10 testes para comprovar a reprodutibilidade do método. RESULTADOS: A utilização de uma força de centrifugação de 300 g por 10 minutos na 1ª. centrifugação e de 640 g por 10 minutos na 2ª. centrifugação obtiveram as maiores concentrações plaquetárias, superiores a 4,5 vezes a concentração na amostra, e os testes foram reprodutíveis. CONCLUSÕES: Uma alta concentração plaquetária pode ser obtida por este protocolo de obtenção de plasma rico em plaquetas e fatores de crescimento e a formação do gel é possível através da utilização de trombina autóloga, também obtida pelo protocolo descrito, facilitando sua utilização em cirurgia plástica, onde vem mostrando bons resultados na cicatrização de feridas e na integração de enxertos ósseos e cutâneos.BACKGROUND: Platelet-rich plasma is an autologous concentration of human platelets in a small volume of plasma. The vast majority of publications reports a significant healing enhancement following its use. This study aim to establish a low-cost method to prepare a platelet-rich plasma and growth factors to be used in plastic surgery. METHODS: Blood was submitted to two centrifugations to obtain platelet-rich plasma. Twenty tests were performed changing the intensity and time of centrifugation, aim to establish the method that achieves the optimal platelet enrichment; and ten tests were performed to confirm the reproducibility of this method. RESULTS: The optimal platelets enrichment, over 4.5 times baseline, values was obtained using 300 g for 10 minutes on the first centrifugation and

  18. Management of knee osteoarthritis by combined stromal vascular fraction cell therapy, platelet-rich plasma, and musculoskeletal exercises: a case series

    Directory of Open Access Journals (Sweden)

    Gibbs N

    2015-11-01

    Full Text Available Nathan Gibbs,1 Rod Diamond,2 Eric O Sekyere,3 Wayne D Thomas4 1South Sydney Sports Medicine, Kensington, 2Diamond Health Care, Kensington, 3Endeavour College of Natural Health, Sydney, 4Cell-Innovations Pty Ltd, Liverpool, NSW, Australia Introduction: Knee osteoarthritis is associated with persistent joint pain, stiffness, joint deformities, ligament damage, and surrounding muscle atrophy. The complexity of the disease makes treatment difficult. There are no therapeutic drugs available to halt the disease progression, leaving patients dependent on pain medication, anti-inflammatory drugs, or invasive joint replacement surgery. Case presentations: Four patients with a history of unresolved symptomatic knee osteoarthritis were investigated for the therapeutic outcome of combining an exercise rehabilitation program with intra-articular injections of autologous StroMed (ie, stromal vascular fraction cells concentrated by ultrasonic cavitation from lipoaspirate and platelet-rich plasma (PRP. The Knee Injury and Osteoarthritis Outcome Score questionnaire (KOOS was administered along with physical function tests over a 12-month period. The first patient achieved a maximum therapeutic outcome of 100 in all five KOOS subscales (left knee, and 100 for four subscales (right knee. The second patient scored 100 in all five KOOS subscales (left knee, and greater than 84 in all subscales (right knee. Treatment of the third patient resulted in improved outcomes in both knees of >93 for four KOOS subscales, and 60 for the Function in Sport and Recreation subscale. The fourth patient improved to 100 in all five KOOS subscales. In all patients, the physical function “Get-up and Go” test and “Stair Climbing Test” returned to normal (a value of zero. Conclusion: This case series indicates that improved outcomes may be obtained when autologous stromal vascular fraction (StroMed cell therapy is combined with traditional exercise practices and PRP for

  19. An inside-out vein graft filled with platelet-rich plasma for repair of a short sciatic nerve defect in rats

    OpenAIRE

    Kim, Ji Yeong; Jeon, Woo Joo; Kim, Dong Hwee; Rhyu, Im Joo; Kim, Young Hwan; Youn, Inchan; Park, Jong Woong

    2014-01-01

    Platelet-rich plasma containing various growth factors can promote nerve regeneration. An inside-out vein graft can substitute nerve autograft to repair short nerve defects. It is hypothesized that an inside-out vein graft filled with platelet-rich plasma shows better effects in the repair of short sciatic nerve defects. In this study, an inside-out vein autograft filled with platelet-rich plasma was used to bridge a 10 mm-long sciatic nerve defect in rats. The sciatic nerve function of rats ...

  20. The use of platelet rich plasma in the treatment of immature tooth with periapical lesion: a case report

    OpenAIRE

    Güven Polat, Günseli; Yıldırım, Ceren; AKGÜN, Özlem Martı; ALTUN, Ceyhan; Dinçer, Didem; Özkan, Cansel Köse

    2014-01-01

    This study describes the treatment of an immature permanent tooth with periapical lesion which was treated with regenerative approach using platelet rich plasma (PRP). The root canal of immature human permanent tooth with periapical lesion was gently debrided of necrotic tissue and disinfected with 2.5% NaOCl, and then medicated with triple antibiotic paste comprised of ciprofloxacin, metronidazole, and tetracycline. When the tooth was asymptomatic, PRP and mineral trioxide aggregate (MTA) we...

  1. Activated platelet-rich plasma improves adipose-derived stem cell transplantation efficiency in injured articular cartilage

    OpenAIRE

    Pham, Phuc Van; Bui, Khanh Hong-Thien; Ngo, Dat Quoc; Vu, Ngoc Bich; Truong, Nhung Hai; Phan, Nhan Lu-Chinh; Le, Dung Minh; Duong, Triet Dinh; Nguyen, Thanh Duc; Le, Vien Tuong; Phan, Ngoc Kim

    2013-01-01

    Introduction Adipose-derived stem cells (ADSCs) have been isolated, expanded, and applied in the treatment of many diseases. ADSCs have also been used to treat injured articular cartilage. However, there is controversy regarding the treatment efficiency. We considered that ADSC transplantation with activated platelet-rich plasma (PRP) may improve injured articular cartilage compared with that of ADSC transplantation alone. In this study, we determined the role of PRP in ADSC transplantation t...

  2. Cytokine, chemokine, and growth factor profile of platelet-rich plasma.

    Science.gov (United States)

    Mussano, F; Genova, T; Munaron, L; Petrillo, S; Erovigni, F; Carossa, S

    2016-07-01

    During wound healing, biologically active molecules are released from platelets. The rationale of using platelet-rich plasma (PRP) relies on the concentration of bioactive molecules and subsequent delivery to healing sites. These bioactive molecules have been seldom simultaneously quantified within the same PRP preparation. In the present study, the flexible Bio-Plex system was employed to assess the concentration of a large range of cytokines, chemokines, and growth factors in 16 healthy volunteers so as to determine whether significant baseline differences may be found. Besides IL-1b, IL-1ra, IL-4, IL-6, IL-8, IL-12, IL-13, IL-17, INF-γ, TNF-α, MCP-1, MIP-1a, RANTES, bFGF, PDGF, and VEGF that were already quantified elsewhere, the authors reported also on the presence of IL-2, IL-5, IL-7, IL-9, IL-10, IL-15 G-CSF, GM-CSF, Eotaxin, CXCL10 chemokine (IP-10), and MIP 1b. Among the most interesting results, it is convenient to mention the high concentrations of the HIV-suppressive and inflammatory cytokine RANTES and a statistically significant difference between males and females in the content of PDGF-BB. These data are consistent with previous reports pointing out that gender, diet, and test system affect the results of platelet function in healthy subjects, but seem contradictory when compared to other quantification assays in serum and plasma. The inconsistencies affecting the experimental results found in literature, along with the variability found in the content of bioactive molecules, urge further research, hopefully in form of randomized controlled clinical trials, in order to find definitive evidence of the efficacy of PRP treatment in various pathologic and regenerative conditions. PMID:26950533

  3. Platelet activation: ultrastructure and morphometry in platelet-rich plasma of horses

    Directory of Open Access Journals (Sweden)

    Bruna M. Zandim

    2012-01-01

    Full Text Available This study was conducted to investigate the activation ability of the platelet-rich plasma (PRP by pharmacological agents, as well as to verify the need or not of this activation for therapeutic use. The PRP was obtained from four healthy crossbred geldings aged 13 to 16 years (15±1years, and was processed for observation and quantification of the platelet morphology by using the transmission electron microscopy. All PRP samples were activated with 10% calcium chloride (CaCl2 solution, pure bovine thrombin or associated with CaCl2. The control (pure PRP was not pharmacologically activated. In the pure PRP samples, 49% of the platelets were classified as state of activation uncertain, 41% as resting, 9% as fully activated and 1% as irreversibly damaged. Treatment with 10% CaCl2 provided a distribution of 54% platelets in state of activation uncertain, 24% as fully activated, 20% as resting, and 2% as irreversibly damaged. The platelet morphology of the bovine thrombin treated samples did not fit into classification adopted, as showing irregular shape with emission of large filamentous pseudopods, appearance of ruptured and whole granules in the remaining cytoplasm and extracellular environment. There was effect of the treatment on the platelet morphology (P=0.03. The 10% CaCl2 is an adequate platelet-activating agent. However, in cases the use of PRP under its liquid form is necessary, the use of pure PRP is recommended, since besides presenting an adequate percentage of fully activated platelets it also has significant amount of the resting type, which can be activated by substances found in the injured tissue.

  4. COMPARATIVE STUDY OF EFFICACY OF PLATELET RICH PLASMA VERSUS MINOXIDIL (5%-10% IN THE TREATMENT OF ANDROGENETIC ALOPECIA IN MALES

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    Pawan Kumar

    2016-01-01

    Full Text Available AIM To compare the efficacy of platelet rich plasma versus minoxidil (5%-10% in the treatment of androgenetic alopecia in males. MATERIAL AND METHODS Two hundred and twenty male patients of age group 20-50 year, clinically diagnosed as androgenetic alopecia of grade II to VII (Norwood-Hamilton Classification were included in the study and randomly divided in two groups. First group was treated by platelet rich plasma and second group was treated by Minoxidil. Response was assessed till 6 months followed by every 15 days visit, on the basis of investigator assessment and photographic assessment. STATISTICAL METHODS Fisher test and Chi-square test. RESULTS Good response was seen in 76% cases with the platelet rich plasma and 48% with minoxidil. CONCLUSION Platelet rich plasma can be an effective form of treatment in androgenetic alopecia than minoxidil.

  5. Effects of administration of adipose-derived stromal vascular fraction and platelet-rich plasma to dogs with osteoarthritis of the hip joints.

    Science.gov (United States)

    Upchurch, David A; Renberg, Walter C; Roush, James K; Milliken, George A; Weiss, Mark L

    2016-09-01

    OBJECTIVE To evaluate effects of simultaneous intra-articular and IV injection of autologous adipose-derived stromal vascular fraction (SVF) and platelet-rich plasma (PRP) to dogs with osteoarthritis of the hip joints. ANIMALS 22 client-owned dogs (12 placebo-treated [control] dogs and 10 treated dogs). PROCEDURES Dogs with osteoarthritis of the hip joints that caused signs of lameness or discomfort were characterized on the basis of results of orthopedic examination, goniometry, lameness score, the Canine Brief Pain Inventory (CBPI), a visual analogue scale, and results obtained by use of a pressure-sensing walkway at week 0 (baseline). Dogs received a simultaneous intraarticular and IV injection of SVF and PRP or a placebo. Dogs were examined again 4, 8, 12, and 24 weeks after injection. RESULTS CBPI scores were significantly lower for the treatment group at week 24, compared with scores for the control group. Mean visual analogue scale score for the treatment group was significantly higher at week 0 than at weeks 4, 8, or 24. Dogs with baseline peak vertical force (PVF) in the lowest 25th percentile were compared, and the treatment group had a significantly higher PVF than did the control group. After the SVF-PRP injection, fewer dogs in the treated group than in the control group had lameness confirmed during examination. CONCLUSIONS AND CLINICAL RELEVANCE For dogs with osteoarthritis of the hip joints treated with SVF and PRP, improvements in CBPI and PVF were evident at some time points, compared with results for the control group. PMID:27580105

  6. 纤维蛋白胶复合自体骨髓与人工骨促进脊柱融合的现状与展望%Fibrin sealant combined with autologous bone marrow and artificial bone to promote spine fusion

    Institute of Scientific and Technical Information of China (English)

    蔡志刚; 芮钢

    2011-01-01

    背景:如何利用纤维蛋白胶的黏合性在术中复合自体骨髓与人工骨来提高脊柱融合的成功率,得进一步研究.目的:综述纤维蛋白胶的研究背景、成分、作用原理及理化特性,维蛋白胶及自体骨髓复合人工骨在脊柱融合中应用的现状.方法:由第一作者检索1994/2010 CNKI系列数据库及PubMed数据库有关纤维蛋白胶的研究背景、成分、作用原理及理化特性,维蛋白胶在构建组织工程骨及修复骨缺损中的应用,体骨髓复合人工骨移植在修复骨缺损中的应用,柱融合中植骨方法应用等方面的文献.结果与结论:自体骨髓复合人工骨移植修复骨缺损在临床取得了较好的疗效,此在脊柱融合中利用自体骨髓复合人工骨来提高脊柱融合率应该是可行的方案,以往自体骨髓混合人工骨的过程相对简单,由于自体骨髓流动性大,射后容易流失,显降低了自体骨髓的成骨作用.因此设想在临床手术中,用纤维蛋白胶的黏合特性将自体骨髓与人工骨黏合在一起,入脊柱关节突、横突部位,分发挥骨髓的最大成骨作用,将是临床一个提高脊柱融合率的简易、快速、有效的方法,待进一步的深入研究.%BACKGROUND: How to use the adhesion of fibrin glue to combine with autologous bone marrow and artificial bone to increase the success rate of spinal fusion in surgery, it is worthy of further study.OBJECTIVE: To review the research background, composition, function theory, physical and chemical properties of fibrin glue,the present of fibrin glue, autologous bone marrow combined with artificial bone in spinal fusion.METHODS: China Knowledge Resources Library-CNKI Series Database (1994 to 2010) and PubMed database were retrieved by the first author for literatures concerning the research background, composition, function theory and physical and chemical properties of fibrin glue, the application of fibrin glue in bone tissue engineering

  7. Aulogous fibrin sealant (Vivostat ® in the neurosurgical practice: Part II: Vertebro-spinal procedures

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    Francesca Graziano

    2016-01-01

    Conclusions: In this preliminary study, the application of autologous fibrin sealant with Vivostat ® resulted in rapid hemostasis and/or acted as an effective dural sealant. Although this product appears to be safe and effective, further investigations are warranted.

  8. Experimental study on autologous of rabbit adipose - derived stem cells transplantation%兔脂肪干细胞增强脂肪移植效果的实验研究

    Institute of Scientific and Technical Information of China (English)

    杨涛; 杨勇; 王艳; 胡晓光

    2014-01-01

    Objective:To establish an animal model for the injectable transPlantation fat tissue transPlantation and to investigate the morPhological changes of rejection after ear transPlantation in rabbit. Methods:All 24 healthy New Zealand white rabbits were divided into four grouPs randomly. The autologous adiPose granule(AG)were imPlanted in the ears of the rabbits as the exPeriment grouP A(n = 6). The autologous adiPose granule(AG)combined with Platelet - rich fibrin(PRF)were imPlanted in ears as the exPeriment grouP B(n = 6). The autologous adiPose gran-ule(AG)combined with autologous adiPose - derived stem cells(ADSCs)were imPlanted in ears as the exPeriment grouP C(n = 6). The autologous adiPose granule(AG)combined with Platelet - rich fibrin(PRF)and autologous adiPose - derived stem cells(ADSCs)were imPlanted in the ears were the control grouP D(n = 6). At month 1,3 and 6 after transPlantation,the survival rates of transPlanted ears,eE staining,rabbit ears light transmission exPeri-ments were Performed. Results:At month 1,3 and 6 after transPlantation,the survival rates of transPlanted ears,eE staining,rabbit ears light transmission exPeriments,the differences of the grouP D and grouP A,B,C were statistical significant(P ﹤ 0. 05). Conclusion:The adiPose granule(AG)combined with Platelet - rich fibrin(PRF)and adi-Pose - derived stem cells(ADSCs)can imProve the survival rate of transPlanted fat tissue and Provide exPerimental basis for clinical fat transPlantation.%目的:建立兔耳脂肪移植模型,观察兔脂肪干细胞( adiPose - derived stem cells,ADSCs)复合脂肪颗粒(adiPose granule,AG)和富血小板纤维蛋白(Platelet - rich fibrin,PRF)移植后的形态学变化,为临床脂肪干细胞移植提供实验依据。方法:以健康新西兰家兔为实验动物,共取24只,随机分成4组(n =6):A 组移植物为 AG;B 组 AG + PRF;C 组 AG + ADSCs;D 组 AG + PRF + ADSCs。在术后1、3、6个月,用 B

  9. Method to obtain platelet-rich plasma from rabbits (Oryctolagus cuniculus

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    Josiane M. Pazzini

    2016-01-01

    Full Text Available Abstract: Platelet-rich plasma (PRP is a product easy and inxpesnsive, and stands out to for its growth factors in tissue repair. To obtain PRP, centrifugation of whole blood is made with specific time and gravitational forces. Thus, the present work aimed to study a method of double centrifugation to obtain PRP in order to evaluate the effective increase of platelet concentration in the final product, the preparation of PRP gel, and to optimize preparation time of the final sample. Fifteen female White New Zealand rabbits underwent blood sampling for the preparation of PRP. Samples were separated in two sterile tubes containing sodium citrate. Tubes were submitted to the double centrifugation protocol, with lid closed and 1600 revolutions per minute (rpm for 10 minutes, resulting in the separation of red blood cells, plasma with platelets and leucocytes. After were opened and plasma was pipetted and transferred into another sterile tube. Plasma was centrifuged again at 2000rpm for 10 minutes; as a result it was split into two parts: on the top, consisting of platelet-poor plasma (PPP and at the bottom of the platelet button. Part of the PPP was discarded so that only 1ml remained in the tube along with the platelet button. This material was gently agitated to promote platelets resuspension and activated when added 0.3ml of calcium gluconate, resulting in PRP gel. Double centrifugation protocol was able to make platelet concentration 3 times higher in relation to the initial blood sample. The volume of calcium gluconate used for platelet activation was 0.3ml, and was sufficient to coagulate the sample. Coagulation time ranged from 8 to 20 minutes, with an average of 17.6 minutes. Therefore, time of blood centrifugation until to obtain PRP gel took only 40 minutes. It was concluded that PRP was successfully obtained by double centrifugation protocol, which is able to increase the platelet concentration in the sample compared with whole blood

  10. Inflammatory response in chronic degenerative endometritis mares treated with platelet-rich plasma.

    Science.gov (United States)

    Reghini, Maria Fernanda S; Ramires Neto, Carlos; Segabinazzi, Lorenzo G; Castro Chaves, Maria Manoela B; Dell'Aqua, Camila de Paula F; Bussiere, Maria Clara C; Dell'Aqua, José Antonio; Papa, Frederico O; Alvarenga, Marco Antonio

    2016-07-15

    Degenerative changes of the endometrium are directly related to age and fertility in mares. Chronic degenerative endometritis (CDE) is correlated with uterine fluid retention and reduced ability to clear uterine inflammation. Recent research in the areas of equine surgery and sports medicine has shown that platelet-rich plasma (PRP) treatment acts as an immunomodulator of the inflammatory response. Therefore, the aim of this study was to determine if the uterine infusion of PRP could modulate the local inflammatory response and modify the intrauterine NO concentrations after artificial insemination (AI) in both normal mares and those with CDE. Thirteen mares with endometrium classified as grade III on the histology (mares with CDE) and eight mares with endometrial histological classification I or II-a normal mares were selected to investigate the effect of PRP therapy. The mares were inseminated with fresh semen in two consecutive cycles in a crossover study design. Thereby, each mare served as its own control and the treatment was performed with intrauterine PRP infusion four hours after AI. The percentage of neutrophils in uterine cytology (CIT, %), uterine fluid accumulation observed on ultrasonography (FLU, mm) and nitric oxide concentration of uterine fluid (NO, μM) were analyzed before and 24 hours after AI. The results reported that mares with CDE (CIT, 68.3 ± 3.27, FLU, 10.7 ± 1.61) have a higher (P  0.05) between categories of mares. In treated cycles with PRP, the intrauterine inflammatory response decrease (P < 0.05) in CDE mares (CDE: CIT, 31.4 ± 6.48, FLU, 5.5 ± 1.28; normal mares: CIT, 13.5 ± 4.31, FLU, 0) when compared with nontreated cycle (CDE: CIT, 68.3 ± 3.27, FLU, 10.7 ± 1.61; NM: CIT, 24.4 ± 3.56, FLU, 0), but did not modify NO concentrations in uterine fluid. Thus, we can conclude that PRP was effective in modulating the exacerbated uterine inflammatory response to semen in mares with CDE but did not reduce NO

  11. Regeneration of mandibular ameloblastoma defect with the help of autologous dental pulp stem cells and buccal pad of fat stromal vascular fraction.

    Science.gov (United States)

    Manimaran, K; Sharma, Rohini; Sankaranarayanan, S; Perumal, S Mahendra

    2016-01-01

    Ameloblastoma is benign odontogenic tumor, which is locally aggressive in behavior. Till date, the treatment of choice is resection and reconstruction using a variety of modalities. Inadequate resection may lead to many complications such as bone deformity and dysfunction. This report is about a 14-year-old male with ameloblastoma treated with autologous dental pulp stem cells (DPSCs) and stromal vascular fraction (SVF) and evidence of bone regeneration. Marsupialization was performed; tooth was extracted and sent for DPSC cultivation. On the day of surgery, SVF was processed from buccal pad of fat, and platelet-rich fibrin (PRF) was prepared from patient's peripheral blood. During the procedure, labial plate resection and curating of tumor lining were done. After which, a mesh packed with SyboGraft T-plug, prepared SVF, DPSCs, and PRF were placed over lingual cortex and pressure dressing was done. After the 1(st) month of surgery the postoperative course was uneventful, the wound shrinkage led to exposure of mesh in the intraoral region. Removal of exposed mesh was done. The correction surgery with removal of part of mesh and primary closure was achieved with SyboGraft plug, SVF and PRF. Enhanced bone formation was seen in post-operative OPG and CT Scan after 10(th) month. In this article, we propose an innovative approach to manage these cases by using a combination of autologous DPSC and buccal pad of fat SVF to regenerate a mandibular defect left by the resection of an ameloblastoma with 1.5 year follow-up. We were able to demonstrate bone regeneration using this technique with no recurrence of tumor. PMID:27563616

  12. 富血小板血浆临床应用及其生物材料性能%Clinical application and biomaterial properties of platelet-rich plasma

    Institute of Scientific and Technical Information of China (English)

    李明; 张长青

    2011-01-01

    BACKGROUND:The preparation methods, equipment and application means of platelet-rich plasma (PRP) have been considerably developed, but PRP is still a relatively new biotechnology for the domestic workers.OBJECTIVE:To illustrate and discuss the concepts and existed problems of PRP, to clearly explicit the preparation and application technology of PRP.METHODS:A search across the databases of Pubmed and VIP (from September 1995 to September 2010) was performed, with key words of "platelet rich plasma, preparation" in English and in Chinese.RESULTS AND CONCLUSION:According to the number of white blood cells contained in PRP, PRP can be divided into pure PRP and leukocyte-rich PRP; PRP can also be divided into non-activated PRP and activated PRP depending on the application form. Activated PRP mainly includes PRP gel and releasates. Platelet-rich fibrin, which is regarded as second-generation platelet concentrate, and PRP both contain high concentrations of platelets, but they are fundamentally different in the preparation method and gel formation method. Because of its safety and easy preparation, PRP will be more and more applied in clinical medicine. However, clinical application should be cautious, because many aspects of PRP are still lack of related research.%背景:富血小板血浆的制备方法、设备及应用手段等方面有了长足发展,但是相对于国内临床工作者而言富血小板血浆技术仍是一项相对较新的生物技术.目的:对富血小板血浆分类等相关概念和存在问题加以说明和论述,使其制备和应用技术等更加清晰和明确.方法:以"platelet rich plasma,preparation,富血小板血浆,制备"为检索词,检索PubMed数据库及维普数据库1995-09/ 2010-09相关文章.结果与结论:根据所含白细胞的多少可以将富血小板血浆分为贫白细胞的富血小板血浆和富白细胞的富血小板血浆;根据应用形式又可以分为未激活富血小板血

  13. Angiogenic factor-enriched platelet-rich plasma enhances in vivo bone formation around alloplastic graft material

    OpenAIRE

    Kim, Eun-Seok; Kim, Jae-Jin; Park, Eun-Jin

    2010-01-01

    PURPOSE Although most researchers agree that platelet-rich plasma (PRP) is a good source of autogenous growth factors, its effect on bone regeneration is still controversial. The purpose of this study was to evaluate whether increasing angiogenic factors in the human PRP to enhance new bone formation through rapid angiogenesis. MATERIAL AND METHODS In vitro, the human platelets were activated with application of shear stress, 20 µg/ml collagen, 2 mM CaCl2 and 10U thrombin/1 × 109 platelets. L...

  14. Platelet-rich plasma may prevent titanium-mesh exposure in alveolar ridge augmentation with anorganic bovine bone

    OpenAIRE

    Torres García-Denche, Jesús; Tamimi, Faleh; Alkhraisat, Mohammad Hamdan; Manchon, Angel; Linares, Rafael; Prados Frutos, J. C.; Hernández, Gonzalo; López Cabarcos, Enrique

    2010-01-01

    Objective: Bone augmentation with the titanium-mesh (Ti-mesh) technique is susceptible to a large rate of complications such as morbidity of bone graft donor site, and mesh exposure to the oral cavity. The purpose of this study was to evaluate the effectiveness of anorganic bovine bone (ABB) in alveolar bone augmentation with the Ti-mesh technique. In addition, we investigated the effect of platelet-rich plasma (PRP) in preventing mesh exposure by using it to cover the Ti-mesh. Patients a...

  15. 富含血小板血浆凝胶的超微结构%Microstructure of Platelet-rich Plasma Gel

    Institute of Scientific and Technical Information of China (English)

    马健; 李放; 任大江; 叶超群

    2011-01-01

    目的 观察富含血小板血浆(PRP)凝胶的超微结构.方法 采用二次离心法制备PRP,分别计数全血和PRP血小板,用酶联免疫吸附法测定全血和PRP凝胶中转移生长因子(TGF)-β1及血小板源性生长因子(PDGF)-AB的浓度;同时,对PRP凝胶行大体观察、HE染色、透射及扫描电镜观察.结果 PRP中的血小板浓度达到全血的4.58倍;PRP凝胶中含有高浓度的TGF-β1、PDGF-AB;扫描电镜及透射电镜观察均显示PRP凝胶主要由大量纤维蛋白网和血小板构成.结论 PRP可能是构建可注射组织工程髓核的理想支架材料.%Objective To observe the microstructure and ultrastructure of platelet-rich plasma gel. Methods PRP gel samples were obtained by two-step centrifugation. The platelets were counted before and after centrifugation. TGF-β1, PDGF-AB were measured in the PRP gel and the whole blood using the enzyme-linked immunosorbent assay. PRP gel samples were observed with macroscopic observation, HE staining, transmission (TEM) and scanning electron microscopy (SEM). Results The platelet concentration of PRP was 458% of whole blood. TGF-β1, PDGF-AB were found in high concentrations in PRP gel samples. Both SEM and TEM showed that PRP gel mainly contained fibrillar material with striated band similar to fibrin filaments, and platelet. Conclusion PRP gel may be an ideal injectable scaffold material for constructing tissue engineering nucleus pulposus.

  16. O uso do plasma rico em plaquetas associado ao aspirado de medular óssea na osteotomia tibial tipo puddu The use of platelet rich plasma enriched with bone marrow aspirate in puddu tibial osteotomy

    Directory of Open Access Journals (Sweden)

    Caio Oliveira D'Elia

    2009-01-01

    Full Text Available OBJETIVO: O presente estudo procurou avaliar a aplicação do plasma rico em plaquetas associado ao aspirado de medular óssea como substituto ósseo ao enxerto autólogo do ilíaco nas osteotomias tibiais proximais de cunha de adição medial (OTCAM. MÉTODOS: Foram estudados 25 pacientes submetidos a OTCAM divididos de forma randomizada em dois grupos, grupo ilíaco, 14 pacientes submetidos a OTCAM nos quais se utilizou o enxerto autólogo do ilíaco para preencher o sitio da osteotomia, grupo PRP, 11 pacientes submetidos a OTCAM nos quais se utilizou um composto formado por plasma rico em plaquetas associado ao aspirado de medular ósseo para preencher o sitio da osteotomia. Foram avaliados o sangramento (variação dos níveis de hemoglobina e hematócrito e a dor (escala visual analógica -EVA, comparando os grupos em relação a essas variáveis. RESULTADOS: Não foram observadas diferenças entre os grupos no que se refere à variação dos níveis de hemoglobina (p = 0,820 e hematócrito (p = 0,323. Os grupos não foram diferentes em relação à intensidade da dor segundo a EVA (p = 0,538. CONCLUSÃO: O uso do PRP associado ao aspirado de medular óssea nas OTCAM não demonstrou vantagem sobre a utilização do enxerto autólogo do ilíaco no que se refere a dor e sangramento.OBJECTIVE: The present study was performed in order to evaluate the use of platelet rich plasma associated to bone marrow aspirate, substituting autologous iliac bone graft in medial opening wedge osteotomy (OWHTO. METHODS: Twenty-five patients were submitted to tibial opening wedge osteotomy, being divided into two groups. Iliac group: 14 patients submitted to OWHTO, using autologous iliac bone graft to fill the gap. PRP group: 11 patients using platelet rich plasma associated to bone marrow aspirate to fill the gap. We evaluated bleeding (hemoglobin and hematocrit levels and pain (visual analogic scale-VAS, then we compared the groups regarding these variables

  17. Platelet Rich Plasma and Hyaluronic Acid Blend for the Treatment of Osteoarthritis: Rheological and Biological Evaluation.

    Directory of Open Access Journals (Sweden)

    Fabrizio Russo

    Full Text Available Osteoarthritis (OA is the most common musculoskeletal disease. Current treatments for OA are mainly symptomatic and inadequate since none results in restoration of fully functional cartilage. Hyaluronic Acid (HA intra-articular injections are widely accepted for the treatment of pain associated to OA. The goal of HA viscosupplementation is to reduce pain and improve viscoelasticity of synovial fluid. Platelet-rich plasma (PRP has been also employed to treat OA to possibly induce cartilage regeneration. The combination of HA and PRP could supply many advantages for tissue repair. Indeed, it conjugates HA viscosupplementation with PRP regenerative properties. The aim of this study was to evaluate the rheological and biological properties of different HA compositions in combination with PRP in order to identify (i the viscoelastic features of the HA-PRP blends, (ii their biological effect on osteoarthritic chondrocytes and (iii HA formulations suitable for use in combination with PRP.HA/PRP blends have been obtained mixing human PRP and three different HA at different concentrations: 1 Sinovial, 0.8% (SN; 2 Sinovial Forte 1.6% (SF; 3 Sinovial HL 3.2% (HL; 4 Hyalubrix 1.5% (HX. Combinations of phosphate buffered saline (PBS and the four HA types were used as control. Rheological measurements were performed on an Anton PaarMCR-302 rheometer. Amplitude sweep, frequency sweep and rotational measurements were performed and viscoelastic properties were evaluated. The rheological data were validated performing the tests in presence of Bovine Serum Albumin (BSA up to ultra-physiological concentration (7%. Primary osteoarthritic chondrocytes were cultured in vitro with the HA and PRP blends in the culture medium for one week. Cell viability, proliferation and glycosaminoglycan (GAG content were assessed.PRP addition to HA leads to a decrease of viscoelastic shear moduli and increase of the crossover point, due to a pure dilution effect. For viscosupplements

  18. A Preliminary Evaluation of Lyophilized Gelatin Sponges, Enhanced with Platelet-Rich Plasma, Hydroxyapatite and Chitin Whiskers for Bone Regeneration

    Directory of Open Access Journals (Sweden)

    Andrew J. Spence

    2013-04-01

    Full Text Available The purpose of this study was to perform a number of preliminary in vitro evaluations on an array of modified gelatin gel sponge scaffolds for use in a bone graft application. The gelatin gels were modified through the addition of a number of components which each possess unique properties conducive to the creation and regeneration of bone: a preparation rich in growth factors (PRGF, a bioactive, lyophilized form of platelet-rich plasma, hydroxyapatite, and chitin whiskers. Platelet-rich plasma therapy is an emerging practice that has proven effective in a number of clinical applications, including enhancing bone repair through improved deposition of new bony matrix and angiogenesis. As such, the inclusion of PRGF in our gelatin scaffolds was intended to significantly enhance scaffold bioactivity, while the addition of hydroxyapatite and chitin whiskers were anticipated to increase scaffold strength. Additionally, the gelatin sponges, which readily dissolve in aqueous solutions, were subjected to 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC cross-linking, either during or post-gelation, to control their rate of degradation. Scaffolds were evaluated in vitro with respect to compressive strength, mass loss/degradation, protein release, and cellular interaction, with results demonstrating the potential of the gelatin gel sponge scaffold for use in the regeneration of bone.

  19. Platelet-rich plasma, plasma rich in growth factors and simvastatin in the regeneration and repair of alveolar bone.

    Science.gov (United States)

    Rivera, César; Monsalve, Francisco; Salas, Juan; Morán, Andrea; Suazo, Iván

    2013-12-01

    Platelet preparations promote bone regeneration by inducing cell migration, proliferation and differentiation in the area of the injury, which are essential processes for regeneration. In addition, several studies have indicated that simvastatin (SIMV), widely used for the treatment of hypercholesterolemia, stimulates osteogenesis. The objective of this study was to evaluate the effects of treatment with either platelet-rich plasma (PRP) or plasma rich in growth factors (PRGF) in combination with SIMV in the regeneration and repair of alveolar bone. The jaws of Sprague Dawley rats (n=18) were subjected to rotary instrument-induced bone damage (BD). Animals were divided into six groups: BD/H2O (n=3), distilled water without the drug and alveolar bone damage; BD/H2O/PRP (n=3), BD and PRP; BD/H2O/PRGF (n=3), BD and PRGF; BD/SIMV (n=3), BD and water with SIMV; BD/SIMV/PRP (n=3), BD, PRP and SIMV; and BD/SIMV/PRGF (n=3), BD, PRGF and SIMV. Conventional histological analysis (hematoxylin and eosin staining) revealed that the BD/SIMV group showed indicators for mature bone tissue, while the BD/SIMV/PRP and BD/SIMV/PRGF groups showed the coexistence of indicators for mature and immature bone tissue, with no statistical differences between the platelet preparations. Simvastatin did not improve the effect of platelet-rich plasma and plasma rich in growth factors. It was not possible to determine which platelet preparation produced superior effects.

  20. Single dose of intra-muscular platelet rich plasma reverses the increase in plasma iron levels in exercise-induced muscle damage:A pilot study

    Institute of Scientific and Technical Information of China (English)

    Zekine Punduk; Onur Oral; Nadir Ozkayin; Khalid Rahman; Rana Varol

    2016-01-01

    Background: Platelet rich plasma (PRP) therapy is widely used in enhancing the recovery of skeletal muscle from injury. However, the impact of intramuscular delivery of PRP on hematologic and biochemical responses has not been fully elucidated in exercise-induced muscle damage. The purpose of this investigation the effects of intramuscular delivery of PRP on hematologic and biochemical responses and recovery strategy muscle damage induced by high intensity muscle exercise (exercise-induced muscle damage, EIMD). Methods: Moderately active male volunteers participated in this study and were assigned to a control group (control, n=6) and PRP administration group (PRP, n=6). The subjects performed exercise with a load of 80%one repetition maximum (1RM) maximal voluntary contraction of the elbow flexors until point of exhaustion of the non-dominant arm was reached. The arms were treated with saline or autologous PRP post-24 h EIMD. Venous blood samples were obtained in the morning to establish a baseline value and 1–4 days post-exercise and were analyzed for serum ferritin, iron, iron binding capacity (IBC), creatinine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), and alanine aminotransferase (ALT). Results: The baseline levels of plasma iron, ferritin, IBC, CK, LDH, AST, and ALT were similar in both the control and PRP groups. However, 24-h following exercise a significant increase in these parameters was observed in both groups between 1 and 4 days during the recovery period. Interestingly, PRP administration decreased plasma iron levels compared to the control on the second day post-exercise. Plasma IBC increased in PRP group from Days 2 to 4 post-exercise compared to the control group whilst PRP administration had no effect on plasma ferritin, CK, AST, ALT, or LDH. Conclusion: Acute exhaustive exercise increased muscle damage markers, including plasma iron, IBC, and ferritin levels, indicating muscle damage induced by exercise. PRP

  1. Regenerative surgery of the complications with Morton's neuroma surgery: use of platelet rich plasma and hyaluronic acid.

    Science.gov (United States)

    De Angelis, Barbara; Lucarini, Lucilla; Orlandi, Fabrizio; Agovino, Annarita; Migner, Alessia; Cervelli, Valerio; Izzo, Valentina; Curcio, Cristiano

    2013-08-01

    Morton's neuroma is an entrapment neuropathy of the plantar digital nerve. We treated five patients with wound dehiscence and tendon exposure, after Morton's neuroma surgery excision using a dorsal approach. In this article we describe our technique. From July 2010 to August 2011, at the Department of Plastic and Reconstructive Surgery, University of Rome 'Tor Vergata', five patients (four females and one male), with ages ranging between 35 and 52 years, were treated with a combination of PRP (platelet rich plasma) and HA (hyaluronic acid). Thirty days following surgery, all patients showed a complete healing of the wound. The use of this technique for the treatment of postoperative wound dehiscence and tendon exposure has proven as satisfactory. PMID:22694086

  2. Analysis of efficiency of the double- centrifugation protocol to prepare platelet rich plasma (PRP – an experimental study in rabbits

    Directory of Open Access Journals (Sweden)

    Michel Reis MESSORA

    2009-09-01

    Full Text Available Introduction and objective:The purpose of this study was to evaluatethe concentrations of platelets obtained from platelet rich plasma (PRP prepared according to the double-centrifugation protocol. Material and methods: Eight adult male rabbits (White New Zealand weighing 2.8 to 4 kg were used.After general anesthesia, 10 ml of blood were drawn from each animal via cardiac puncture. Each blood sample was centrifuged according to the protocol of Sonnleitner et al.(2000.The peripheral blood (total from each animal and the PRP samples platelets were counted manually. Data were submitted to statistical analysis.The normality of the data was confirmed and the Student’s t test was applied (p < 0.05.Results: PRP samples presented an average platele count significantly higher than that of peripheral blood.Conclusion Within the limits of this study, it was concluded that the double centrifugation protocol was adequate to prepare PRP .

  3. US definitions, current use, and FDA stance on use of platelet-rich plasma in sports medicine.

    Science.gov (United States)

    Beitzel, Knut; Allen, Donald; Apostolakos, John; Russell, Ryan P; McCarthy, Mary Beth; Gallo, Gregory J; Cote, Mark P; Mazzocca, Augustus D

    2015-02-01

    With increased utilization of platelet-rich plasma (PRP), it is important for clinicians to understand the United States, the Food and Drug Administration (FDA) regulatory role and stance on PRP. Blood products such as PRP fall under the prevue of FDA's Center for Biologics Evaluation and Research (CBER). CBER is responsible for regulating human cells, tissues, and cellular and tissue-based products. The regulatory process for these products is described in the FDA's 21 CFR 1271 of the Code of Regulations. Under these regulations, certain products including blood products such as PRP are exempt and therefore do not follow the FDA's traditional regulatory pathway that includes animal studies and clinical trials. The 510(k) application is the pathway used to bring PRP preparation systems to the market. The 510(k) application allows devices that are "substantially equivalent" to a currently marketed device to come to the market. There are numerous PRP preparation systems on the market today with FDA clearance; however, nearly all of these systems have 510(k) clearance for producing platelet-rich preparations intended to be used to mix with bone graft materials to enhance bone graft handling properties in orthopedic practices. The use of PRP outside this setting, for example, an office injection, would be considered "off label." Clinicians are free to use a product off-label as long as certain responsibilities are met. Per CBER, when the intent is the practice of medicine, clinicians "have the responsibility to be well informed about the product, to base its use on firm scientific rationale and on sound medical evidence, and to maintain records of the product's use and effects." Finally, despite PRP being exempted, the language in 21 CFR 1271 has caused some recent concern over activated PRP; however to date, the FDA has not attempted to regulate activated PRP. Clinicians using activated PRP should be mindful of these concerns and continued to stay informed. PMID

  4. Intra-Articular Injections of Platelet-Rich Plasma versus Hyaluronic Acid in the Treatment of Osteoarthritic Knee Pain: A Randomized Clinical Trial in the Context of the Spanish National Health Care System

    Science.gov (United States)

    Montañez-Heredia, Elvira; Irízar, Sofia; Huertas, Pedro J.; Otero, Esperanza; del Valle, Marta; Prat, Isidro; Díaz-Gallardo, Macarena S.; Perán, Macarena; Marchal, Juan A.; Hernandez-Lamas, María del Carmen

    2016-01-01

    Intra-articular injection of platelet-rich plasma (PRP) has been established as a suitable treatment for knee osteoarthritis. Here, we present a double-blind randomized controlled clinical trial, conducted in a public Hospital of the Spanish National Health Care System, to evaluate the efficacy of injecting autologous PRP versus hyaluronic acid (HA) in knee osteoarthritis. PRP was manufactured in Malaga’s Regional Blood Center (Spain). Patients that met the eligibility criteria were randomized into a PRP group or a HA group. Pain and functional improvements were assessed pre- and post-treatment (three and six months follow-up) using the Visual Analogue Scale (VAS); the Knee and Osteoarthritis Outcome System (KOOS) scale and the European Quality of Life scale (EUROQOL). Both groups presented pain reduction at six months. The VAS scores for the PRP group improved by at least 50% from their initial value, particularly at three months following the final infiltration, with results resembling those of the HA group at six months. PRP was more effective in patients with lower osteoarthritis grades. Both treatments improved pain in knee osteoarthritis patients without statistically significant differences between them. However, PRP injection was proved to improve pain three months after the final infiltration and to be more effective in lower osteoarthritis grades. PMID:27384560

  5. Morphogen and proinflammatory cytokine release kinetics from PRGF-Endoret fibrin scaffolds: evaluation of the effect of leukocyte inclusion.

    Science.gov (United States)

    Anitua, E; Zalduendo, M M; Prado, R; Alkhraisat, M H; Orive, G

    2015-03-01

    The potential influence of leukocyte incorporation in the kinetic release of growth factors from platelet-rich plasma (PRP) may explain the conflicting efficiency of leukocyte platelet-rich plasma (L-PRP) scaffolds in tissue regeneration. To assess this hypothesis, leukocyte-free (PRGF-Endoret) and L-PRP fibrin scaffolds were prepared, and both morphogen and proinflammatory cytokine release kinetics were analyzed. Clots were incubated with culture medium to monitor protein release over 8 days. Furthermore, the different fibrin scaffolds were morphologically characterized. Results show that leukocyte-free fibrin matrices were homogenous while leukocyte-containing ones were heterogeneous, loose and cellular. Leukocyte incorporation produced a significant increase in the contents of proinflammatory cytokines interleukin (IL)-1β and IL-16 but not in the platelet-derived growth factors release (<1.5-fold). Surprisingly, the availability of vascular endothelial growth factor suffered an important decrease after 3 days of incubation in the case of L-PRP matrices. While the release of proinflammatory cytokines was almost absent or very low from PRGF-Endoret, the inclusion of leukocytes induced a major increase in these cytokines, which was characterized by the presence of a latent period. The PRGF-Endoret matrices were stable during the 8 days of incubation. The inclusion of leukocytes alters the growth factors release profile and also increased the dose of proinflammatory cytokines.

  6. Effect of platelet-rich plasma on the healing of intrabony defects treated with an anorganic bovine bone mineral and expanded polytetrafluoroethylene membranes.

    NARCIS (Netherlands)

    Dori, F.; Huszar, T.; Nikolidakis, D.; Arweiler, N.B.; Gera, I.; Sculean, A.

    2007-01-01

    BACKGROUND: Regenerative periodontal therapy with a combination of platelet-rich plasma (PRP) + an anorganic bovine bone mineral (ABBM) + guided tissue regeneration (GTR) has been shown to result in significantly higher probing depth reductions and clinical attachment level gains compared to treatme

  7. Effect of platelet-rich plasma on the healing of intrabony defects treated with an anorganic bovine bone mineral: a pilot study.

    NARCIS (Netherlands)

    Dori, F.; Kovacs, V.; Arweiler, N.B.; Huszar, T.; Gera, I.; Nikolidakis, D.; Sculean, A.

    2009-01-01

    BACKGROUND: Periodontal therapy using the combination of platelet-rich plasma (PRP) and different grafting materials has been suggested as a modality to enhance the outcome of regenerative surgery. In most clinical studies, a barrier membrane was used to cover the defects, and thus, the effects of P

  8. The Use of Platelet Rich Plasma, Bone Morphogenetic Protein-2 and Different Scaffolds in Oral and Maxillofacial Surgery - Literature Review in Comparison with Own Clinical Experience

    Directory of Open Access Journals (Sweden)

    Karl-Heinz Schuckert

    2011-01-01

    Full Text Available Objectives: The purpose of this article was to review and critically assess the use of platelet rich plasma, recombinant human bone morphogenetic protein-2 and different scaffolds (i.e. tricalciumphosphate, polycaprolactone, demineralized bone matrix and anorganic bovine bone mineral in oral and maxillofacial surgery comparing the relevant literature and own clinical experience.Material and Methods: A literature review was conducted using MEDLINE, MEDPILOT and COCHRANE DATABASE OF SYSTEMATIC REVIEWS. It concentrated on manuscripts and overviews published in the last five years (2006-2010. The key terms employed were platelet rich plasma, bone morphogenetic proteins and their combinations with the above mentioned scaffolds. The results of clinical studies and animal trials were especially emphasized. The statements from the literature were compared with authors’ own clinical data.Results: New publications and overviews demonstrate the advantages of platelet rich plasma in bone regeneration. The results from the literature review were discussed and compared with the publications detailing authors’ own experiences.Conclusions: A favourable outcome concerning newly grown bone was achieved combining platelet rich plasma in addition to optimal matrices with or without recombinant human bone morphogenetic protein-2, depending on the clinical case. As a consequence, the paradigm shift from transplantation of autogenous bone to bone tissue engineering appears promising.

  9. Evaluation of bone regenerative capacity in rats claverial bone defect using platelet rich fibrin with and without beta tri calcium phosphate bone graft material

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    Walid Ahmed Abdullah

    2016-07-01

    Conclusion: The addition of β-TCP to PRF significantly improved bone regeneration in the first 2 weeks after surgery. Although the differences between results with and without the addition of β-TCP to PRF were statistically insignificant from weeks 3 to 6, it was nevertheless apparent that the group receiving the combination showed better results. We suggest a synergistic mechanism for this effect.

  10. Surgical re-entry evaluation of regenerative efficacy of bioactive Gengigel™ and platelet-rich fibrin in the treatment of grade II furcation: A novel approach

    OpenAIRE

    Gurkirat Kaur Sandhu; Paramjit Kaur Khinda; Amarjit Singh Gill; Harveen Singh Kalra

    2015-01-01

    The furcation area creates situations in which routine periodontal procedures are somewhat limited, and surgical procedures are generally required. The introduction of bioactive agents, such as platelet concentrates, enamel matrix derivatives, bone morphogenic proteins, and matrix macromolecules such as hyaluronic acid has expanded the scope for better outcomes in furcation treatment. Hyaluronic acid is a naturally occurring nonsulfated high molecular weight glycosaminoglycan that forms a cri...

  11. Clinical and histopathological studies using fibrin-rich plasma in the treatment of bisphosphonate-related osteonecrosis of the jaw.

    Science.gov (United States)

    Dincă, Octavian; Zurac, Sabina; Stăniceanu, Florica; Bucur, Mihai Bogdan; Bodnar, Dana Cristina; Vlădan, Cristian; Bucur, Alexandru

    2014-01-01

    The authors report their experience using platelet-rich fibrin (PRF) therapy for the treatment of ten patients presenting bisphosphonate-related osteonecrosis of the jaw (BRONJ). The aim of our study was to evaluate the effect of this therapy on recurrent BRONJ and to describe the clinical and histopathological/immunohistochemical staining features of PRF treatment. As such, we describe the method we used and report the results observed in the areas treated as well as side effects. The reported results recommend the safety and efficacy of PRF in treatment of BRONJ.

  12. FIBRIN GLUE DAN APLIKASINYA

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    Agi Harliani S

    2015-08-01

    Full Text Available Fibrin Tissue Adhesive (FTA, Fibrin Sealant (FS or Fibrin Glue (FG are names given to a group of product that lead to the formation of fibrin clot at the site of application. Fibrin Glue represents a new revolution for local haemostatic, which produced by based on the understanding about blood coagulation process. The mechanism of FG mimics the last stage of blood coagulation process. Haemophilia, is a congenital inherited bleeding disorder, characterized by repeated bleeding episodes. The basic pathology is deficiency of factor VIII (hemophilia A or factor IX (hemophilia B. At bleeding episodes, hemophilia patients need replacement therapy. Hemophilia patients need transfusion of cryoprecipitate, Fresh Frozen Plasma (FFP or factor concentrate as replacement therapy. Oral surgery, dental extraction, circumcision, and orthopedic operations are the most important indications for fibrin glue in hemophilia care. As haemostatic local, FG minimizes bleeding, reducing the need of transfusion or factor concentrate, reducing the complication of transfusion, hospitalization and cost.

  13. 自体富含血小板血浆凝胶治疗Ⅳ期褥疮探讨%Efficacy of platelet-rich plasma gel in the treatment of stage Ⅳ pressure ulcer

    Institute of Scientific and Technical Information of China (English)

    邹国友; 郑闽前; 贾伟涛; 徐小卒; 殷俊; 王栋梁

    2014-01-01

    Objective To observe the effects of platelet-rich plasma (PRP) gel in the treatment of [Ⅳ stage pressure ulcers.Methods A total of 12 patients with stage Ⅳ pressure ulcers were treated with PRP gel from December 2012 to December 2013 in our department.The PRP gel was formed by autologous PRP mixed with thrombin and calcium chloride.The PRP gel was applied to the wound after dressing.Time intervals between dressing were 4 days.Overall pressure ulcer improvement was assessed every week until complete wound healing.Results All stage Ⅳ pressure ulcer wound infection was controlled and fresh granulation tissue was found after 2 times of PRP gel covering.The time of ulcer wound healing were 6-10 weeks (mean 8 weeks).The times of changing the PRP gel were 16.No patients experienced adverse reactions during treatment.Conclusions PRP gel can effectively control the pressure ulcer infection and promote ulcer wound healing.%目的 观察自体富含血小板血浆(platelet-rich plasma,PRP)凝胶在Ⅳ期褥疮创面中的作用. 方法 2012年12月至2013年12月我科共计12例Ⅳ期褥疮患者,取患者自身外周静脉血20 ml,2次离心制作自体PRP,将其与凝血酶-钙剂按比例混合形成凝胶,放置于创面.每4d更换PRP凝胶,观察创面的愈合情况. 结果 所有Ⅳ期褥疮创面在2次换药感染得到控制并且肉芽组织新鲜.创面完全愈合平均8周,更换PRP敷料平均16次.治疗过程中无患者出现不良反应. 结论 PRP能够有效的控制褥疮创面的感染和促进创面的愈合.

  14. Effects of platelet-rich plasma gel on skin healing in surgical wound in horses Efeitos do gel de plasma rico em plaquetas na cicatrização de feridas cirúrgicas na pele de cavalos

    Directory of Open Access Journals (Sweden)

    Rafael DeRossi

    2009-08-01

    Full Text Available PURPOSE: To establish a low-cost method to prepare platelet-rich plasma (PRP and evaluates the potential of platelet derived factors to enhance wound healing in the surgical wounds in equine. METHODS: To obtain a PRP gel, calcium gluconate and autologous thrombin were added to platelet-rich plasma. For the tests six saddle horses were used and two surgical incisions were made in each animal. Wounds were treated with PRP gel or untreated. Sequential wound biopsies collected at Treatment 1: at days 5 and 30 and Treatment 2: at days 15 and 45 post wounding permitted comparison of differentiation markers and wound repair. RESULTS: The optimal platelets enrichment over 4.0 time's baseline values was obtained using 300 g for 10 min on the first centrifugation and 640 g for 10 min on the second centrifugation. CONCLUSION: Wounds treated with PRP gel exhibit more rapid epithelial differentiation and enhanced organization of dermal collagen compared to controls in equineOBJETIVO: Estabelecer um método econômico na preparação de plasma rico em plaquetas (PRP e avaliar se os fatores derivados destas plaquetas aceleram a cicatrização de feridas cirúrgicas em cavalos. MÉTODOS: Gluconato de cálcio e trombina autógena foram adicionados ao PRP para a obtenção do gel de PRP. Foram usados seis cavalos de sela, cada um dos quais sofreu duas incisões cirúrgicas. Uma destas incisões foi tratada com gel de PRP e a outra suturada de maneira tradicional (controle. A biópsia das feridas foi coletada de maneira seqüencial; Tratamento 1. nos dias 5 e 30 e Tratamento 2. nos dias 15 e 45 do período pós-operatório permitindo uma comparação na diferenciação epitelial e no reparo das feridas. RESULTADOS: O enriquecimento das plaquetas obtido através de uma primeira centrifugação usando 300 g por 10 minutos e uma segunda 640 g por 10 minutos acelerou quatro vezes a reparação tecidual em relação ao controle. CONCLUSÃO: As feridas tratadas com gel

  15. Therapeutic efficacy of connective tissue autotransplants with periosteum and platelet rich plasma in the management of gingival recession

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    Jovičić Bojan

    2013-01-01

    Full Text Available Background/Aim. Gingival recession progression in clinical practaice has influenced the development of various surgical procedures and techniques for solving esthetic imperfections and subjective difficulties coused by gingival recession. The aim of this study was to verify efficacy of surgical procedures and to compare both of surgical procedures through the keratinized tissue width. Methods. The study included 20 teeth with gingival recesion, Müller class I and II. Ten teeth with gingival recession were treated with connective tissue autotransplants with periosteum in combination with coronary guided surgical flap (CTG group. On the contralateral side 10 teeth with gingival recession were treated with the same surgical procedures but in combination with platelet-rich plasma (CTGPRP group. We measured the keratinized tissue width. For statistical significance we used the Student's t-test. Results. The study reveled a statistical significance in reducing vertical deepress of recession by both used treatments. Root deepness in CTG and CTG-PRP group was 90% and 93.5%, respectively. With both surgical techniques we achieved larger zone of keratinized gingiva but with a wide zone of keratinized tissue in CTG - the PRP group. Conclusion. The concept regeneration technique with PRP and with the stimulating influence of platele activated growth factors results in the regeneration of deep periodontal tissue as an important prerequisite for the successful treatment of gingival recession.

  16. Tendon Stem Cells and Platelet-Rich Plasma%Tendon Stem Cells and Piatelet-Rich Plasma

    Institute of Scientific and Technical Information of China (English)

    James H-C.Wang

    2011-01-01

    Acute and chronic tendon injuries(i.e.tendinopathy)are common,yet current treatments cannot restore normal structure and function to injured tendons.This problem mainly stems from our incomplete understanding of tendon cell properties and responses to biomechanical and biochemical environments surrounding the cells.However,significant progress has been made in recent years on two fronts.The first is the new discovery that tendons contain a population of multi-potent tendon stem cells(TSCs).The second is the wide application of platelet-rich plasma(PRP)in orthopaedics and sports medicine to treat injured tendons.This mini-review aims to discuss the studies on TSCs,with a focus on their differential properties with respect to tenocytes and their differential mechano-responses when subjected to small and large mechanical loading conditions.In light of the critical importance of the safety and efficacy of PRP treatment in clinic,basic scientific research related to PRP effects on TSCs,particularly on their differentiation,is also presented.Finally,the urgent need for rigorous basic scientific research to justify PRP applications in clinic settings is proposed.

  17. Effect of Platelet Rich Plasma Combined with Chitosan Biodegradable Film on Full-Thickness Wound Healing in Rat Model

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    Rahim Mohammadi

    2016-01-01

    Full Text Available Objective:To assess the effects of platelet rich plasma (PRP with chitosan biodegradable film on full thickness wound healing in rat. Methods:This was an experimental study being performed in 2015 during a 4-month period. Twenty-four male white Wistar rats were divided into four groups of 12 rats each, randomly: Control group (SHAM with creation of wounds and no treatment, PRP group with creation of wounds and application of one milliliter PRP, Chitosan group (CHIT with dressing the wound with chitosan and CHIT/PRP group with application of one mL PRPand dressing the wound with chitosan. The wounds were created by cutting healthy skin.Wound size was measured on 6, 9, 12, 15, 18 and 21 post surgery and was compared between groups. Results:Reduction in wound area, hydroxyproline contents and biomechanical parametersindicated there was significant difference (p=0.001 between group CHIT/PRP and other groups. Biomechanical testing was performed on day 9 post surgery in incisional model. Quantitative histological studies and mean rank of the qualitative studies demonstrated that there was significant difference (p<0.001 between group CHIT/PRP and other groups. Conclusion:PRP with chitosan have beneficial effects on wounds repair and could be suggested for treating various types of wounds in animals and human being.

  18. A novel collagen/platelet-rich plasma (COL/PRP) scaffold: preparation and growth factor release analysis.

    Science.gov (United States)

    Zhang, Xiujie; Wang, Jingwei; Ren, Mingguang; Li, Lifeng; Wang, Qingwen; Hou, Xiaohua

    2016-06-01

    Platelet-rich plasma (PRP) has been widely used in clinical practice for more than 20 years because it causes the release of many growth factors. However, the burst release pattern and short release period of PRP have become obstacles to its application. An optimal controllable release system is an urgent need for researchers. This study investigated whether collagen/PRP (COL/PRP) scaffolds can serve as a vehicle for the controllable release of growth factors. We fabricated a novel scaffold that integrates PRP activated by thrombin or collagen into type I collagen. The mechanical properties, cytotoxicity, and transforming growth factor β1 (TGF-β1), platelet derived growth factor (PDGF), fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF) content were evaluated. Our results demonstrate that the COL/PRP scaffolds were not cytotoxic to L-929 fibroblasts. The PDGF and FGF content in the thrombin group was at a higher level and lasted for a long period of time. Collagen and thrombin played the same role in the release of TGF-β1 and VEGF. These data suggest that the novel COL/PRP scaffolds provide a carrier for the controllable release of growth factors and may be used in tissue- regenerative therapies.

  19. Platelet-Rich Plasma in the Treatment of Subcutaneous Venous Access Device Scars: A Head-to-Head Patient Survey

    Directory of Open Access Journals (Sweden)

    C. Eichler

    2015-01-01

    Full Text Available Introduction. Platelet-rich plasma (PRP is a product widely used in sports medicine, tissue repair, and general surgery. A recent meta-analysis showed this product to be beneficial when introduced into a wound area, be it intra-articular (i.e., joint-injections or direct introduction onto the wound surface. Methods. Between the years of 2012 and 2014 a questionnaire evaluating surgical outcome after port (venous access device removal was answered by 100 patients in the control group and 20 patients in a PRP group, leading to a total of 120 patients in this single center, retrospective, subjective outcome evaluation. Results. No statistical difference was shown in postsurgical complication rates, postsurgical pain, decreased mobility, and overall quality of life. A significant difference was shown in overall patient satisfaction and the desire to further improve port area scarring. Results differed significantly in favor of the PRP group. Interestingly, approximately 40.2% of patients are dissatisfied with the surgical outcome after port removal in the control group. This result, though surprising, may be improved to 10% dissatisfaction when a PRP product is used. Conclusion. PRP products such as Arthrex ACP are safe to use and present an additional option in improving surgical outcome.

  20. Platelet-rich plasma: evidence for the treatment of patellar and Achilles tendinopathy--a systematic review.

    Science.gov (United States)

    Di Matteo, B; Filardo, G; Kon, E; Marcacci, M

    2015-04-01

    Platelet-rich plasma (PRP) has been introduced in the clinical practice to treat a growing number of different musculoskeletal pathologies. It is currently applied in the treatment of Achilles and patellar tendinopathies, which are common sport-related injuries very challenging to manage. Aim of the present paper was to review systematically the available clinical evidence concerning the application of PRP in the treatment of patellar and Achilles tendinopathy. A systematic review of the literature was performed according to the following inclusion criteria for relevant articles: (1) clinical reports of any level of evidence, (2) written in the English language, (3) with no time limitation and (4) on the use of PRP to treat conservatively Achilles and patellar tendinopathy. Twenty-two studies were included and analyzed. Two studies on patellar tendinopathy were randomized controlled trials (RCTs), whereas just one RCT was published on Achilles tendon. All the papers concerning patellar tendon reported positive outcome for PRP, which proved to be superior to other traditional approaches such as shock-wave therapy and dry needling. In the case of Achilles tendon, despite the encouraging findings reported by case series, the only RCT available showed no significant clinical difference between PRP and saline solution. The main finding of this study was the paucity of high-level literature regarding the application of PRP in the management of patellar and Achilles tendinopathy. However, the clinical data currently available, although not univocal, suggest considering PRP as a therapeutic option for recalcitrant patellar and Achilles tendinopathies. PMID:25323041

  1. Binding of thrombin-activated platelets to a fibrin scaffold through α(IIbβ₃ evokes phosphatidylserine exposure on their cell surface.

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    Tomasz Brzoska

    Full Text Available Recently, by employing intra-vital confocal microscopy, we demonstrated that platelets expose phosphatidylserine (PS and fibrin accumulate only in the center of the thrombus but not in its periphery. To address the question how exposure of platelet anionic phospholipids is regulated within the thrombus, an in-vitro experiment using diluted platelet-rich plasma was employed, in which the fibrin network was formed in the presence of platelets, and PS exposure on the platelet surface was analyzed using Confocal Laser Scanning Microscopy. Almost all platelets exposed PS after treatment with tissue factor, thrombin or ionomycin. Argatroban abrogated fibrin network formation in all samples, however, platelet PS exposure was inhibited only in tissue factor- and thrombin-treated samples but not in ionomycin-treated samples. FK633, an α(IIbβ₃ antagonist, and cytochalasin B impaired platelet binding to the fibrin scaffold and significantly reduced PS exposure evoked by thrombin. Gly-Pro-Arg-Pro amide abrogated not only fibrin network formation, but also PS exposure on platelets without suppressing platelet binding to fibrin/fibrinogen. These results suggest that outside-in signals in platelets generated by their binding to the rigid fibrin network are essential for PS exposure after thrombin treatment.

  2. Calidad del plasma rico en plaquetas: estudio de la activación plaquetaria Platelet-rich plasma quality: a study on platelet activation

    Directory of Open Access Journals (Sweden)

    C. Sáez-Torres Barroso

    2007-08-01

    Full Text Available Objetivo. El plasma rico en plaquetas (PRP es utilizado de forma cada vez más frecuente en técnicas quirúrgicas de regeneración tisular. No obstante, el procesamiento de la sangre hasta obtener PRP puede desencadenar la activación prematura de las plaquetas y la pérdida de los factores bioactivos. En este trabajo estudiamos la calidad de los concentrados de plaquetas obtenidos siguiendo la técnica de doble centrifugación en tubo. Método. Se someten 50 ml de sangre a una primera centrifugación a 200g 10 minutos, se recoge el sobrenadante y se centrifuga a 700g 15 minutos. Posteriormente, tras eliminar las 2/3 partes del plasma, se resuspenden las plaquetas y se analiza el grado de enriquecimiento, el estado de activación y la reserva funcional de las plaquetas. Resultados. El enriquecimiento en plaquetas del PRP fue de 364±177% (n=45 respecto de los niveles presentes en sangre total. Mediante el estudio de la expresión de CD62 por citometría de flujo se determinó el porcentaje de plaquetas activadas en las muestras de 8 donantes. Mientras que en la sangre no procesada se detectó un 2,7% de plaquetas activadas, tras la preparación del PRP éste era sólo de 3,6%, aumentando hasta el 16% en el concentrado almacenado toda la noche a 22º C. Tras la estimulación con trombina el porcentaje de plaquetas activadas fue de 96,2%. Conclusión. Este protocolo de preparación de PRP no produce una activación significativa de las plaquetas. La respuesta a la estimulación con trombina de los concentrados indica un buen estado de reserva plaquetaria.Objective. Platelet Rich Plasma (PRP is an autologous preparation currently used in oral and maxillofacial reconstructive surgery. Blood collection and preparation of platelet concentrates may lead to platelet activation and the premature loss of their granular load. In this study, we have analyzed the quality of the PRP obtained from a small volume of whole blood through a double centrifugation

  3. Transplante autólogo de conjuntiva com uso de cola de fibrina em pterígios primários Conjunctival autologous transplantation using fibrin glue in primary pterygium

    Directory of Open Access Journals (Sweden)

    Ramon Coral-Ghanem

    2010-08-01

    complicações. Apesar de resultar em menor taxa de recidiva conjuntival, esta técnica não parece diminuir significativamente a incidência de recidiva corneana.PURPOSE: To assess the rate of recurrence and complications after primary pterygium removal with conjunctival autograft using fibrin tissue adhesive. Secondarily, to compare these results with a retrospective series that underwent the same surgery using sutures. METHODS: A hundred and six eyes of 100 patients who underwent surgery with fibrin glue were prospectively studied, while 58 eyes of 51 patients who underwent surgery with sutures were retrospectively evaluated. Data included: age, gender, eye involved, location, grade and pterygium invasion and post-operative complications. Patients were followed for at least 5 months. RESULTS: Among 106 eyes undergoing surgery with fibrin glue, 12 (11.3% had recurrence, including 4 (3.8% conjunctival and 8 (7.5% corneal. Mean age was statistically inferior in the recurrence cases compared to others (mean of 34.6 vs. 43.7; P=0.033. The time for recurrence ranged from 1.6 to 13.1 months (mean of 4.4. Among the corneal recurrences, 2 eyes had invasion beyond the limbus of up to 0.5 mm and 6 eyes from 0.6 to 1.0 mm. No eye underwent reoperation. Other complications included: transitory granuloma formation in 3 cases, partial graft detachment or slippage in 3 and dellen in 1. In the suture group, 15 eyes (25.9% had recurrence, including 8 conjunctival (13.8% and 7 (12.1% corneal. The time for recurrence ranged from 0.7 to 9.7 months (mean 4.5 months. The conjunctival recurrence rate was statistically inferior in the glue group compared to the suture (P=0.023, however no statistically significant difference was observed in relation to corneal recurrence (P=0.232. CONCLUSION: The use of fibrin glue as an alternative to sutures in pterygium surgery was associated with good results and few complications. Despite a decrease in conjunctival recurrence rates, this technique does not

  4. Platelet-Rich Plasma and Adipose-Derived Mesenchymal Stem Cells for Regenerative Medicine-Associated Treatments in Bottlenose Dolphins (Tursiops truncatus)

    OpenAIRE

    Richard J Griffeth; Daniel García-Párraga; Maravillas Mellado-López; Jose Luis Crespo-Picazo; Mario Soriano-Navarro; Alicia Martinez-Romero; Victoria Moreno-Manzano

    2014-01-01

    Dolphins exhibit an extraordinary capacity to heal deep soft tissue injuries. Nevertheless, accelerated wound healing in wild or captive dolphins would minimize infection and other side effects associated with open wounds in marine animals. Here, we propose the use of a biological-based therapy for wound healing in dolphins by the application of platelet-rich plasma (PRP). Blood samples were collected from 9 different dolphins and a specific and simple protocol which concentrates platelets gr...

  5. Platelet-rich plasma in orthopedic therapy: a comparative systematic review of clinical and experimental data in equine and human musculoskeletal lesions

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    Brossi, Patrícia M.; Moreira, Juliana J; Machado, Thaís SL; Baccarin, Raquel YA

    2015-01-01

    Background This systematic review aimed to present and critically appraise the available information on the efficacy of platelet rich plasma (PRP) in equine and human orthopedic therapeutics and to verify the influence of study design and methodology on the assumption of PRP’s efficacy. We searched Medline, PubMed, Embase, Bireme and Google Scholar without restrictions until July 2013. Randomized trials, human cohort clinical studies or case series with a control group on the use of PRP in te...

  6. Platelet-Rich Plasma and Skeletal Muscle Healing: A Molecular Analysis of the Early Phases of the Regeneration Process in an Experimental Animal Model

    OpenAIRE

    Ivan Dimauro; Loredana Grasso; Simona Fittipaldi; Cristina Fantini; Neri Mercatelli; Silvia Racca; Stefano Geuna; Alessia Di Gianfrancesco; Daniela Caporossi; Fabio Pigozzi; Paolo Borrione

    2014-01-01

    Platelet-rich plasma (PRP) has received increasing interest in applied medicine, being widely used in clinical practice with the aim of stimulating tissue healing. Despite the reported clinical success, there is still a lack of knowledge when considering the biological mechanisms at the base of the activity of PRP during the process of muscle healing. The aim of the present study was to verify whether the local delivery of PRP modulates specific molecular events involved in the early stages o...

  7. Bone Tissue Engineering with Adipose-Derived Stem Cells in Bioactive Composites of Laser-Sintered Porous Polycaprolactone Scaffolds and Platelet-Rich Plasma

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    Han-Tsung Liao; Jyh-Ping Chen; Ming-Yih Lee

    2013-01-01

    Three-dimensional porous polycaprolactone (PCL) scaffolds with consistent inter-pore channels, 83% porosity and 300–400 μm pore size were fabricated via selective laser sintering. The PCL scaffold was combined with platelet-rich plasma (PRP) to form a bioactive composite and studied for potential application in bone tissue engineering using porcine adipose-derived stem cells (PASCs). The PCL/PRP/PASCs construct showed enhanced cell seeding efficiency and synergistically increased the differen...

  8. Efficacy of Platelet-Rich-Plasma (PRP) and Highly Purified Bovine Xenograft (Laddec®) Combination in Bone Regeneration after Cyst Enucleation: Radiological and Histological Evaluation

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    Pappalardo, Sabrina; Guarnieri, Renzo

    2013-01-01

    ABSTRACT Objectives The purpose of the present study was to evaluate the efficacy of adding platelet-rich plasma (PRP) to a new highly purified bovine allograft (Laddec®) in the bone regeneration of cystic bony defects augmented following cystectomy. Material and Methods Study sample included 20 patients undergoing cystectomy in which the bone defect was filled with PRP and Laddec®. All patients were examined with periapical radiographs before operation and at follow-up. After 3 months, at re...

  9. The Use of Platelet Rich Plasma, Bone Morphogenetic Protein-2 and Different Scaffolds in Oral and Maxillofacial Surgery - Literature Review in Comparison with Own Clinical Experience

    OpenAIRE

    Karl-Heinz Schuckert; Stefan Jopp; Magdalena Osadnik

    2011-01-01

    ABSTRACT Objectives The purpose of this article was to review and critically assess the use of platelet rich plasma, recombinant human bone morphogenetic protein-2 and different scaffolds (i.e. tricalciumphosphate, polycaprolactone, demineralized bone matrix and anorganic bovine bone mineral) in oral and maxillofacial surgery comparing the relevant literature and own clinical experience. Material and Methods A literature review was conducted using MEDLINE, MEDPILOT and COCHRANE DATABASE OF SY...

  10. Evaluation of immediately loaded dental implants bioactivated with platelet-rich plasma placed in the mandibular posterior region: A clinico-radiographic study

    OpenAIRE

    Anand, Ullas; Mehta, D. S.

    2012-01-01

    Background and Objectives: The purpose of the present study was to clinically and radiographically assess the soft and hard tissue changes around the immediately loaded single tooth implants bioactivated with platelet-rich plasma (PRP), placed in the mandibular posterior region. Materials and Methods: A total of 11 patients having single tooth edentulous space in the mandibular posterior region were selected. An endosseous implant was placed after clinical and radiographic examination in each...

  11. Use of Biomaterials Associated or not to the Platelet-Rich Plasma in Cranial Bone Defects. Microscopical Study in Dogs

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    Edevaldo Tadeu CAMARINI

    2006-08-01

    Full Text Available Objective: This study was to evaluate a possible accelerating activity of the bone repair process through the use platelet-rich plasma (PRP, associated to biomaterials in sockets produced in cranial skull of dogs. Method: Six animals of indefinite race has been selected, females, approach weight of 8 kg, being carried through six sockets of 8 mm with trefine drill that had been divided in six groups: each group it filled with the following materials: Group I: blood (control group; Group II: blood and PRP; Group III: Biogran® (bioactive glass and blood; Group IV: Biogran®, blood and PRP; Group V: Bio-Oss® (natural hidroxiapatite of bovine origin and blood; Group VI: Bio-Oss®, blood and PRP. Thirty and sixty days after procedure the animals had sacrificed themselves and the parts had been fixed in formol 10%, demineralized in EDTA solution, processed and follow to Hematoxilin and Eosin (HE technique. Results: It did not have significant difference between the control groups (Groups I and II. To the 30 days, it had development of the hard tissue repair, however, if it did not observe significant differences between the two biomaterials (Biogran and Bio-Oss. In 60 days, was observed a bone is in mature state, however, if it microscopically did not evidence significant differences between the use or not of the PRP influencing in the tissue repairing. Conclusion: Carried through the microscopical analysis, concluded that the materials had presented osteoconduction property and absence of inflammatory reaction. The presence or not of PRP did not intervene with the evolution of the repair and is necessity the continuity of new scientific inquiries involving the PRP and its applications.

  12. Synergistic anabolic actions of hyaluronic acid and platelet-rich plasma on cartilage regeneration in osteoarthritis therapy.

    Science.gov (United States)

    Chen, Wei-Hong; Lo, Wen-Cheng; Hsu, Wei-Che; Wei, Hong-Jian; Liu, Hen-Yu; Lee, Chian-Her; Tina Chen, Szu-Yu; Shieh, Ying-Hua; Williams, David F; Deng, Win-Ping

    2014-12-01

    Osteoarthritis (OA) is a common disease associated with tissue inflammation, physical disability and imbalanced homeostasis in cartilage. For advanced treatments, biological approaches are currently focused on tissue regeneration and anti-inflammation. This study was undertaken to evaluate the therapeutic efficacies of hyaluronic acid (HA) and platelet-rich plasma (PRP) (HA+PRP) on OA. Articular chondrocytes were obtained from five OA patients. The optimal HA and PRP concentrations were evaluated by MTT assay. The expressions of chondrogenic and inflammatory genes were analyzed by RT-PCR. Signaling pathway was examined by immunoblotting and the expressions of OA pathology-related chemokines and cytokines was demonstrated by real-time PCR-based SuperArray. The therapeutic efficacies of HA+PRP were then demonstrated in 3D arthritic neo-cartilage and ACLT-OA model. Here we showed that HA+PRP could greatly retrieve pro-inflammatory cytokines-reduced articular chondrocytes proliferation and chondrogenic phenotypes, the mechanism of which involve the sequential activation of specific receptors CD44 and TGF-βRII, downstream mediators Smad2/3 and Erk1/2, and the chondrogenic transcription factor SOX9. The real-time PCR-based SuperArray results also indicated that OA pathology-related chemokines and cytokines could be efficiently suppressed by HA+PRP. Moreover, the cartilaginous ECM could be retrieved from inflammation-induced degradation by HA+PRP in both 2D monolayer and 3D neo-cartilage model. Finally, the intra-articular injection of HA+PRP could strongly rescue the meniscus tear and cartilage breakdown and then decrease OA-related immune cells. The combination of HA+PRP can synergistically promote cartilage regeneration and inhibit OA inflammation. This study might offer an advanced and alternative OA treatment based on detailed regenerative mechanisms.

  13. Evaluation of Platelet-Rich Plasma in Combination with Deproteinized Bovine Bone Mineral in the Rabbit Cranium; A Pilot Study

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    MA. Shafiee Ardestani

    2005-12-01

    Full Text Available Statement of Problem: Reconstruction methods are an essential prerequisite for functional rehabilitation of the stomatognathic system. Platelet-rich plasma (PRP offers a new and potentially useful adjunct to bone substitute materials (e.g. Xenografts inoral and maxillofacial bone and implant reconstructive surgery.Purpose: An animal study was carried out to investigate the influence of PRP on the regeneration of non-critical sized bony defects, treated with Deproteinized Bovine Bone Mineral (DBBM.Materials and Methods: Eight New Zealand white rabbits were included in this randomized, pilot study. Three equal cranial bone defects (3×6 mm were created and immediately grafted with DBBM and PRP+DBBM; one of them was left unfilled to serve as a control. The defects were evaluated using histologic and histomorphometricanalysis at 2, 4, 8 and 12 weeks.Results: The histomorphometric findings showed a significant increase in bone area and trabecular maturity in experimental defects as compared to the control at 4, 8 and 12 week intervals. A significant increase in bone formation was seen with the additionof PRP to DBBM at 2, 4 and 8 week intervals. At 12 weeks, the level of bone formation was similar between the two groups. There was also a significant increase in the rate of biodegradation of the DBBM particles with the addition of PRP at 2, 4, 8 and 12 weeks.No foreign body reaction and severe inflammation was seen in any of specimens.Conclusion: Within the limitations of this pilot study, it was concluded that the addition of PRP to Xenogenic bone substitute material in non-critical-sized defects of the rabbit cranium showed a histomorphometric increase in bone formation (until the 8th week ofhealing and a greater amount of biomaterial degradation throughout the study period.

  14. Effectiveness and relevant factors of platelet-rich plasma treatment in managing plantar fasciitis:A systematic review

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    Seet Khing Chiew

    2016-01-01

    Full Text Available Background: Plantar fasciitis (PF is a common foot complaint, affects both active sportsmen and physically inactive middle age group. It is believed that PF results from degenerative changes rather than inflammation. Platelet-rich plasma (PRP therapy has been introduced as an alternative therapy for PF. This study is aimed to systematically review to the effectiveness and relevant factors of PRP treatment in managing PF. Materials and Methods: A search was conducted in electronic databases, including PubMed, Scopus, and Google Scholar using different keywords. Publications in English-language from 2010 to 2015 were included. Two reviewers extracted data from selected articles after the quality assessment was done. Results: A total of 1126 articles were retrieved, but only 12 articles met inclusion and exclusion criteria. With a total of 455 patients, a number of potentially influencing factors on the effectiveness of PRP for PF was identified. In all these studies, PRP had been injected directly into the plantar fascia, with or without ultrasound guidance. Steps from preparation to injection were found equally crucial. Amount of collected blood, types of blood anti-coagulant, methods in preparing PRP, speed, and numbers of time the blood samples were centrifuged, activating agent added to the PRP and techniques of injection, were varied between different studies. Regardless of these variations, superiority of PRP treatment compared to steroid was reported in all studies. Conclusion: In conclusion, PRP therapy might provide an effective alternative to conservative management of PF with no obvious side effect or complication. The onset of action after PRP injection also greatly depended on the degree of degeneration.

  15. Platelet-rich plasma effects on grainy fat transplantation%富血小板血浆对颗粒脂肪移植的影响*☆

    Institute of Scientific and Technical Information of China (English)

    李锋; 李昆; 李杰; 田卫东

    2013-01-01

    BACKGROUND:The higher long-term absorption rate greatly influence the widely application of fat transplantation. Platelet-rich plasma contains a high concentration of growth factors, which benefits to the tissue healing and regeneration. OBJECTIVE:To observe the effects of platelet-rich plasma on grainy fat transplantation and to investigate the mechanisms preliminarily. METHODS:Ten 6-week-old nude mice were prepared. The right or left dorsal subcutaneous tissues were randomly selected as the platelet-rich plasma group (0.5 mL fat granule+0.1 mL platelet-rich plasma), and the contralateral side was regarded as the control group (0.5 mL fat granule+0.1 mL phosphate-buffered saline). At 10 and 90 days after implantation, five nude mice were selected from each group, and then the mice were sacrificed to obtain the grafts in each group for general appearance observation, volume determination and histological detection. Furthermore, we isolated adipose-derived stem cells from human subcutaneous fat tissue during the in vitro experiment. Cel counting kit-8 and real-time PCR were used to evaluate the influence of platelet-rich plasma on adipose-derived stem cel proliferation and adipogenic differentiation in vitro, respectively. RESULTS AND CONCLUSION:Comparison of the grafts obtained at 10 and 90 days after implantation, the residual volume in the platelet-rich plasma group was significantly larger than that in the control group (P<0.05), Moreover, more normal adipocytes and capil ary formation were observed in the platelet-rich plasma group (P<0.05). For in vitro experiment, platelet-rich plasma could significantly improve adipose-derived stem cel proliferation, and the expressions of adipogenic-related genes were up-regulated in platelet-rich plasma-induced adipose-derived stem cells. Al results demonstrate that platelet-rich plasma can improve the survival of fat grafts,which might be closely related to that platelet-rich plasma can promote the proliferation and

  16. Analysis of experimental tendinitis in rats treated with laser and platelet-rich plasma therapies by Raman spectroscopy and histometry.

    Science.gov (United States)

    de Carvalho, Paula Kariluce; Silveira, Landulfo; Barbosa, Danillo; Munin, Egberto; Salgado, Miguel Angel Castillo; Villaverde, Antonio Balbin

    2016-01-01

    The objective of this controlled experimental study was to analyze the changes in the Achilles tendons of rats with experimentally induced tendinitis after treatment with platelet-rich plasma (PRP) and/or laser therapies by histometry to quantify fibroblasts and by Raman spectroscopy to determine the biochemical concentration of collagen types I and III. Fifty-four male Wistar rats were divided into six treatment groups: control (G1); PRP only (G2); irradiation with 660 nm laser (G3); irradiation with 830 nm laser (G4); PRP plus 660 nm laser irradiation (G5); and PRP plus 830 nm laser irradiation (G6). Injuries (partial tenotomy) were inflicted in the middle third of the Achilles tendon, with PRP added prior to suture in the appropriate experimental groups. A diode laser (model Laser Flash® III, DMC Equipamentos Ltda, São Carlos, SP, Brazil) that can be operated in two wavelengths 660 and 830 nm was used for irradiation treatments. The irradiation protocol was energy density of 70 J/cm², 20 s irradiation time, and 0.028 cm² spot area, per point in three points in the injured. The histometry was made in micrographical images of the H&E stained sections and evaluated by ImageJ (version 1.46r)®. Raman spectra were collected using a dispersive spectrometer at 830 nm excitation, 200 mW power, and 10 s integration time (P-1 Raman system, Lambda Solutions, Inc. MA, USA). The relative amount of type I collagen was significantly greater in the PRP plus 830 nm laser irradiation group (468 ± 188) than in the control (147 ± 137), 630 nm laser only (191 ± 117), and 830 nm laser only (196 ± 106) groups (p < 0.01), while the quantity of type III collagen was significantly greater in the PRP-only group compared to both irradiated groups without PRP (p < 0.05). Treatment with PRP combined with irradiation at 830 nm resulted in a larger number of fibroblasts and increased concentration of type I collagen, thus accelerating the healing of the injured

  17. Immunohistological Evaluation of Revascularized Immature Permanent Necrotic Teeth Treated by Platelet-Rich Plasma: An Animal Investigation

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    Saeed Moradi

    2016-09-01

    Full Text Available Objective: Pulp regeneration within the root canal of necrotic teeth is considered an ideal treatment to allow for continued root development and recover teeth vitality. This study aims to evaluate the inductive effect of platelet-rich plasma (PRP on expression of angiogenesis factors and pulpal revascularization of immature necrotic teeth. Materials and Methods: In this experimental animal study, we randomly divided 28 immature premolars from two mixed breed dogs into four groups, two experimental, negative and a positive control. Premolars in negative control group were left intact to develop normally. In the positive control and experimental groups, we removed the pulps and induced pulp necrosis, after which the chambers were sealed. Then, we applied the revascularization protocol in the experimental teeth located in the right quadrant. Two months later, the same protocol was applied to the left quadrant. The root canals were disinfected by irrigation with sodium hypochlorite (NaOCl solution and application a triple antibiotic past. Following the induction of a blood clot (BC inside the canal space, the coronal portion of the canals was assigned to either of two experimental groups: group 1 [BC+PRP+ mineral trioxide aggregate (MTA], group 2 (BC+MTA. Access cavities were sealed with a Glass Ionomer. The jaws that held the teeth were processed for histologic analysis of newly formed tissue and immunohistochemical evaluation according to vascular endothelial growth factor (VEGF and factor VIII expressions in the canals. Results: Histological analysis demonstrated no significant difference in the formation of new vital tissue inside the root canals between groups1 (42.8% and 2 (43.5%, P>0.05. Based on immunohistochemical evaluation, micro-vessel density (MVD of the granulation tissues in both groups were similar and were higher compared with the normal pulp. We observed strongly positive expressions of VEGF and factor VIII in the stromal and

  18. Platelet-rich concentrate in serum free medium enhances osteogenic differentiation of bone marrow-derived human mesenchymal stromal cells

    Science.gov (United States)

    Ramasamy, Thamil Selvee; Karunanithi, Puvanan; Naveen, Sangeetha Vasudevaraj; Murali, Malliga Raman; Abbas, Azlina A.; Kamarul, Tunku

    2016-01-01

    Previous studies have shown that platelet concentrates used in conjunction with appropriate growth media enhance osteogenic differentiation of human mesenchymal stromal cells (hMSCs). However, their potential in inducing osteogenesis of hMSCs when cultured in serum free medium has not been explored. Furthermore, the resulting osteogenic molecular signatures of the hMSCs have not been compared to standard osteogenic medium. We studied the effect of infrequent supplementation (8-day interval) of 15% non-activated platelet-rich concentrate (PRC) in serum free medium on hMSCs proliferation and differentiation throughout a course of 24 days, and compared the effect with those cultured in a standard osteogenic medium (OM). Cell proliferation was analyzed by alamar blue assay. Gene expression of osteogenic markers (Runx2, Collagen1, Alkaline Phosphatase, Bone morphogenetic protein 2, Osteopontin, Osteocalcin, Osteonectin) were analyzed using Q-PCR. Immunocytochemical staining for osteocalcin, osteopontin and transcription factor Runx2 were done at 8, 16 and 24 days. Biochemical assays for the expression of ALP and osteocalcin were also performed at these time-points. Osteogenic differentiation was further confirmed qualitatively by Alizarin Red S staining that was quantified using cetylpyridinium chloride. Results showed that PRC supplemented in serum free medium enhanced hMSC proliferation, which peaked at day 16. The temporal pattern of gene expression of hMSCs under the influence of PRC was comparable to that of the osteogenic media, but at a greater extent at specific time points. Immunocytochemical staining revealed stronger staining for Runx2 in the PRC-treated group compared to OM, while the staining for Osteocalcin and Osteopontin were comparable in both groups. ALP activity and Osteocalcin/DNA level were higher in the PRC group. Cells in the PRC group had similar level of bone mineralization as those cultured in OM, as reflected by the intensity of Alizarin red

  19. Plasma rico en plaquetas: Una revisión bibliográfica Platelet Rich Plasma: A review of literature

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    T Beca

    2007-04-01

    Full Text Available Se presenta una revisión bibliográfica de la evolución en la literatura acerca del plasma rico en plaquetas (PRP. Tras una introducción se expone la composición del PRP, aludiendo fundamentalmente a los factores de crecimiento presentes en él para continuar con la secuencia de obtención y empleo quirúrgico del producto. Posteriormente se puede observar una revisión del proceso de regeneración a nivel histológico desde el momento de colocación hasta la sexta. En el apartado de discusión, se pueden observar los distintos estudios a favor y en contra de la utilización del PRP encontrados en la literatura, y a continuación se apuntan los posibles riesgos recientemente atribuibles al empleo del PRP para terminar con una breve revisión de estudios comparativos de diversos métodos de obtención y activación del PRP. Por último, se establecen una serie de conclusiones y se expone la bibliografía consultada.The aim of this work was to make a review about the evolution of the platelet rich plasma (PRP in literature. After an introduction, the main components of the PRP are showed, giving more relevance to the growing factors to continue with the obtaining procedure and its aplication in the surgical aspect. Later, a review about the regeneration process by means of histological findings is made from the very first moment to the sixth week. In the discussion, it can be observed that there is a great polemic between the found studies about the usefulness of the PRP in osseous regeneration, and then some potencial riks about the utilization of the PRP are put forward to finish with a brief review of comparative studies between different obtaining and activation methods. Finally, some conclusions are established and the bibliography searched is presented.

  20. Tendon Derived Stem Cells Promote Platelet-Rich Plasma Healing in Collagenase-Induced Rat Achilles Tendinopathy

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    Lei Chen

    2014-12-01

    Full Text Available Background/Aims: Tendon injuries are common, difficult to cure and usually healed with fibrosis and scar tissue. The aim of this study was to evaluate tendon derived stem cells (TDSCs and platelet rich plasma (PRP in the treatment of collagenase induced Achilles tendinopathy in rat. Methods: Four and 8 weeks (n=18 after TDSCs, PRP, PRP with TDSC or PBS (control injection into collagenase or saline (sham injected rat Achilles tendon, tendon tissue was harvested and tendon quality was evaluated by histology and biomechanical testing. TDSCs were cultured and treated by 10% PRP, and the FAK/ERK1/2 signaling pathway and tenocyte-related genes were detected by western blot analysis. Results: Compared to the control, PRP treatment resulted in better healing of injured tendons with improved histological outcomes and biomechanical functions. The addition of TDSCs to PRP treatment significantly enhanced the effects of PRP treatment alone. TDSC injection alone had little effect on tendon healing. PRP and PRP with TDSC treatments of collagenase induced tendon injuries also increased the mRNA and protein expression of tenocyte-related genes (type I collagen, SCX, Tenascin C and activated the focal adhesion kinase (FAK and extracellular-regulated kinase (ERK 1/2 signaling pathways. Treatment of TDSCs in vitro with 10% PRP significantly increased the phosphorylation levels of FAK and ERK1/2 and the protein levels of tenocyte-related genes (Col I, SCX and Tenascin C. Inhibition of the FAK and ERK1/2 signaling pathways abolished the effect of PRP. Conclusion: This study concludes that PRP combined with TDSCs is potentially effective for the treatment of tendinopathy. The PRP induced, FAK and ERK1/2 dependent activation of tenocyte related genes in TDSCs in vitro suggests that the beneficial healing effect of the PRP with TDSC combination might occur by means of an improved TDSC differentiation toward the tenocyte lineage. Thus, a PRP with TDSC combination

  1. Use of platelet rich plasma to treat plantar fasciitis: design of a multi centre randomized controlled trial

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    Peerbooms Joost C

    2010-04-01

    Full Text Available Abstract Background If conservative treatment for chronic plantar fasciitis fails, often a corticosteroid injection is given. Corticosteroid injection gives temporarily pain reduction, but no healing. Blood platelets initiate the natural healing rate. GPS® gives an eightfold concentrate platelets of patients own blood. Injection of these platelets in the attachment of the fascia to the os calcis might induce a healing rate. Methods and design A randomized controlled multi centre trial will be performed. The study population consists of 120 patients of 18 years and older. Patients with chronic plantar fasciitis will be allocated randomly to have a steroid injection or an autologous platelet concentrate injections. Data will be collected before the procedure, 4,8,12,26 weeks and 1 year after the procedure. The main outcome measures of this study are pain and function measured with questionnaires. Conclusion Recent literature show positive effects for the treatment of tendinosis with autologous platelet injections. The forthcoming trial will compare treatment for chronic plantar fasciitis with a steroid injection versus an autologous platelet injection. Our results will be published as soon as they become available. Trial Registration Trial registration number: http://www.clinicaltrials.gov NCT00758641.

  2. Platelet-rich plasma for treatment of ischiogluteal bursitis%富血小板血浆治疗坐骨结节滑囊炎

    Institute of Scientific and Technical Information of China (English)

    沈梓维; 林子洪; 郑秋坚; 王良泽; 叶圣龙; 李盛; 钱思浓

    2014-01-01

    BACKGROUND:Ischiogluteal bursitis has been recognized for a long time, but its treatment stil limits to local blocking injection and surgery methods that were developed 40 years ago. OBJECTIVE:To observe the efficacy of platelet-rich plasma on ischiogluteal bursitis. METHODS:Data of 15 patients with ischiogluteal bursitis were colected. Al the patients with ischiogluteal bursitis were treated with bilateral platelet-rich plasma (n=10) or local blocking injection (n=5). Patients’ outcomes were assessed by visual analogue scale, the Treatment Satisfaction Questionnaire for Medication (TSQM) Version II and recurrence rate. The folow-up time was from 6 to 14 months. RESULTS AND CONCLUSION: There was no statistical difference in visual analogue scale score between the platelet-rich plasma group and local blocking group (F=0.219,P=0.643), but the score of visual analogue scale in the platelet-rich plasma group was higher during short-term folow-up (within 1 week after treatment), but lower in the long-term folow-up. In the aspects of overal satisfaction score, clinical effectiveness and side effects, the platelet-rich plasma group was inferior to the local blocking group at short-term folow-up, especialy at 1 week after treatment; however, these scores became better in the platelet-rich plasma group than the local blocking group during the long-term folow-up period. In addition, no statistical difference in the convenience score was found between the two groups. At the last folow-up, the recurrence rate in the platelet-rich plasma group was lower than that in the local blocking group. Both the platelet-rich plasma and local blocking injection can significantly reduce the pain of patients with ischiogluteal bursitis. Local blocking injection has better short-term effectiveness. Platelet-rich plasma injection works moderately, but its effectiveness can last for longer time, and the recurrence rate is lower.%背景:坐骨结节滑囊炎被认识长久,

  3. VEGF concentration from plasma-activated platelets rich correlates with microvascular density and grading in canine mast cell tumour spontaneous model

    OpenAIRE

    Patruno, R; Arpaia, N; Gadaleta, CD; Passantino, L.; N. Zizzo; Misino, A; Lucarelli, NM; Catino, A; Valerio, P; Ribatti, D; Ranieri, G.

    2008-01-01

    Abstract Canine cutaneous mast cell tumour (CMCT) is a common cutaneous tumour in dog, with a higher incidence than in human. CMCT is classified in three subgroups, well and intermediately differentiated (G1 and G2), corresponding to a benign disease, and poorly differentiated (G3), corresponding to a malignant disease, which metastasize to lymph nodes, liver, spleen and bone marrow. In this study, we have evaluated serum (S), platelet-poor plasma (P-PP), plasma-activated platelet rich (P-APR...

  4. Successful treatment of athletic pubalgia in a lacrosse player with ultrasound-guided needle tenotomy and platelet-rich plasma injection: a case report.

    Science.gov (United States)

    Scholten, Paul M; Massimi, Stephen; Dahmen, Nick; Diamond, Joanne; Wyss, James

    2015-01-01

    Athletic pubalgia is a syndrome of persistent groin pain due to chronic repetitive trauma or stress involving the pelvic joints and many musculotendinous structures that cross the anterior pelvis. As a result, the differential diagnosis can be complex, but insertional tendinopathies are the most common. This case report describes a novel approach to the treatment of distal rectus abdominis tendinopathies with ultrasound-guided needle tenotomy and platelet-rich plasma (PRP) injection. After injection, the patient returned to pain-free play at his previous level of intensity. This suggests that PRP may be a useful treatment for this diagnosis. PMID:25134854

  5. A novel technique for guided bone regeneration using platelet-rich plasma and osteogenic progenitor cells: Literature-based rationale and case report.

    Science.gov (United States)

    Kwon, TaeHyun; Grieco, Peter C; Levin, Liran; Intini, Giuseppe

    2016-03-01

    Achieving predictable guided bone regeneration in critical size defects for future endosseous dental implant therapy poses a great challenge to clinicians. A novel technique utilizing autogenous osteogenic progenitor cells, calcium sulfate activated platelet-rich plasma in addition to particulate allograft was successfully used to augment a severely deficient maxillary anterior edentulous ridge. After 6 months of healing, satisfactory radiographic and clinical bone gain was noted with significant increase in alveolar ridge width. Endosseous implants were placed and restored successfully. The techniques with underlying clinical and biologic rationales are presented and discussed in this report.

  6. Efficacy of Platelet-Rich-Plasma (PRP) and Highly Purified Bovine Xenograft (Laddec®) Combination in Bone Regeneration after Cyst Enucleation: Radiological and Histological Evaluation

    OpenAIRE

    Sabrina Pappalardo; Renzo Guarnieri

    2013-01-01

    Objectives: The purpose of the present study was to evaluate the efficacy of adding platelet-rich plasma (PRP) to a new highly purified bovine allograft (Laddec®) in the bone regeneration of cystic bony defects augmented following cystectomy.Material and Methods: Study sample included 20 patients undergoing cystectomy in which the bone defect was filled with PRP and Laddec®. All patients were examined with periapical radiographs before operation and at follow-up. After 3 months, at re-entry s...

  7. A novel platelet-rich arterial thrombosis model in rabbits. Simple, reproducible, and dynamic real-time measurement by using double-opposing inverted-sutures model.

    Science.gov (United States)

    Shieh, S J; Chiu, H Y; Shi, G Y; Wu, C M; Wang, J C; Chen, C H; Wu, H L

    2001-09-01

    Though numerous animal thrombosis models have been introduced, an easy, reliable, and reproducible arterial thrombosis model remains a continuing challenge prior to a thrombolytic study. In an effort to evaluate the efficiency of various recombinant thrombolytic agents with specific affinity to activated platelets in vivo, we developed a novel double-opposing inverted-sutures model to create a platelet-rich thrombus in the femoral artery of rabbits. The arteriotomy was done semicircumferentially, and variously sized microsurgical sutures were introduced intraluminally in a double-opposing inverted manner. The animals were divided into three groups according to the double-opposing inverted-sutures used: Group 1 with 10-0 nylon (n=6), Group 2 with 9-0 nylon (n=6), and Group 3 with 8-0 nylon (n=22). The superficial epigastric branch was cannulated with a thin polyethylene (PE) tube for intraarterial administration of the studied thrombolytic agent. The blood flow was continuously measured with a real-time ultrasonic flow meter. Within 2 h of installation of the sutures, there was no thrombus formation in either Group 1 or 2. In Group 3, the thrombosis rate was 91% (20 of 22) under a steady baseline flow (with an average of 12.23+/-2.40 ml/min). It was highly statistically significant with a P-value of .0000743 using Fisher's Exact Test. The averaged time to thrombosis was 21.8+/-9.8 min. The ultrasonic flow meter to record the dynamic real-time measurement of blood flow was a guideline for thrombus formation or dissolution, which was correlated with the morphological findings of stenotic status of the vessel detected by the Doppler sonography. The components of the thrombus were proven to be platelet-rich predominant by histological examination via hematoxylin and eosin (H&E) stain and transmission electron microscopy (TEM). To confirm that the double-opposing inverted-sutures model would be useful for a study of thrombolytic agents, we evaluated the effects of

  8. Effects of platelet-rich plasma on healing of alveolar socket: Split-mouth histological and histometric evaluation in Cebus apella monkeys

    Directory of Open Access Journals (Sweden)

    Pessoa Roberto

    2009-01-01

    Full Text Available Context: The prediction of implant treatment is directly influenced by the quality of the remaining bone after tooth extraction. Aims : The purpose of this experimental study was to, histologically and histometrically, evaluate the bone repair process in the central areas of extraction sockets filled with platelet-rich plasma. Materials and Methods: Four young adult male Cebus apella monkeys were used. The extraction of both right and left inferior second premolars was accomplished. After extraction, in one of the extraction sockets, coagulum was maintained while in the other it was removed; the alveolus was dried with gauze compress and filled up with platelet concentrate. For PRP production, Sonnleitner′s protocol was followed. The specimens for histological and histometric assessment were obtained in 30, 90, 120 and 180 days intervals. Results: In 30 days new bone formation was intense in both experimental and control sockets and no significant differences were observed between the two groups. After 90 days of the extraction, while the control group showed signs of decrease in osteogenesis, in the experimental unit, the process of bone formation and fibroblast-like cell proliferation remained intense. After 120 days, the PRP treated socket was occupied by large trabeculae of bone. After 180 days, the control unit was occupied mostly with bone marrow. The experimental unit remained occupied with large amounts of bone tissue. Conclusions: It was possible to conclude that bone repair was enhanced by the use of platelet- rich plasma in alveolar sockets.

  9. Treatment of endodontically induced periapical lesions using hydroxyapatite, platelet-rich plasma, and a combination of both: An in vivo study

    Directory of Open Access Journals (Sweden)

    C Vaishnavi

    2011-01-01

    Full Text Available Aim and Objectives : To evaluate bone regeneration in endodontically induced periapical lesions using Hydroxyapatite, Platelet-Rich Plasma (PRP, and a combination of Hydroxyapatite and Platelet-Rich Plasma for a period of one year. Materials and Methods : Twenty systemically healthy patients of both genders between the ages 20 and 40 years were included. To qualify, the patient had to have a tooth where non-surgical root canal therapy had failed, periapical radiolucency was present, and periapical root end surgery was required. The bony defect had to be confined to the apical area, with the bone covering the entire root surface coronally, with an intact lingual cortical plate. Patients were randomly divided into four groups, with five patients each, as follows: Group I - Replacement with Hydroxyapatite, Group II - Replacement with PRP, Group III - Replacement with PRP and Hydroxyapatite, and Group IV - Control group with no substitutes. The patients were evaluated both clinically and radiographically. Results : The radiographic evaluation revealed that Group I patients showed complete bone regeneration with evidence of a trabecular pattern, at the end of one year, Group II patients showed complete bone regeneration at the end of nine months, Group III patients showed complete bone regeneration at the end of six months, and Group IV patients showed bone regeneration, which was not satisfactory even after one year. Conclusions : The PRP and Hydroxyapatite combination facilitated better and faster bone regeneration when compared to PRP alone.

  10. Keloid Management: A Retrospective Case Review on a New Approach Using Surgical Excision, Platelet-Rich Plasma, and In-office Superficial Photon X-ray Radiation Therapy

    Science.gov (United States)

    Jones, Michael E.; Hardy, Cherrell; Ridgway, Julie

    2016-01-01

    ABSTRACT OBJECTIVE: The objective of this retrospective study was to evaluate the efficacy of the authors’ combination therapy protocol for keloid treatment. DESIGN: Retrospective. SETTING: Plastic surgery office-based outpatient setting in New York City. PATIENTS: Forty patients with 44 keloid scars requiring surgical excision. INTERVENTIONS: Keloid scars were treated using surgical excision, platelet-rich plasma, and postoperative in-office superficial photon X-ray radiation therapy. Intralesional triamcinolone injections were administered once to 4 patients with poor results on scar scale assessment. Patient follow-up visits ranged from 3 to 11 months to assess for evidence of recurrence and adverse effects. MAIN OUTCOME MEASURE(S): For the purpose of this study, recurrence was defined as any sign of extraordinary erythema, induration, and hypertrophy beyond the site of excision. MAIN RESULTS: In the 16 keloids treated with 2 fractions, there was no evidence of recurrence. One of 25 keloids treated with 3 fractions demonstrated evidence of recurrence. One of 3 keloids treated with a single fraction displayed signs of recurrence. Postirradiation hyperpigmentation was noted in all patients. CONCLUSIONS: Surgical excision combined with platelet-rich plasma and postoperative in-office superficial radiation therapy achieved a 95.5% nonrecurrence rate at 1- to 3-month follow-up. This protocol appears to be a safe and viable option in the management of keloids and merits further randomized controlled study of its comparative efficacy. PMID:27300360

  11. Mice expressing a mutant form of fibrinogen that cannot support fibrin formation exhibit compromised antimicrobial host defense.

    Science.gov (United States)

    Prasad, Joni M; Gorkun, Oleg V; Raghu, Harini; Thornton, Sherry; Mullins, Eric S; Palumbo, Joseph S; Ko, Ya-Ping; Höök, Magnus; David, Tovo; Coughlin, Shaun R; Degen, Jay L; Flick, Matthew J

    2015-10-22

    Fibrin(ogen) is central to hemostasis and thrombosis and also contributes to multiple physiologic and pathologic processes beyond coagulation. However, the precise contribution of soluble fibrinogen vs insoluble fibrin matrices to vascular integrity, tissue repair, inflammation, and disease has been undefined and unapproachable. To establish the means to distinguish fibrinogen- and fibrin-dependent processes in vivo, Fib(AEK) mice were generated that carry normal levels of circulating fibrinogen but lack the capacity for fibrin polymer formation due to a germ-line mutation in the Aα chain thrombin cleavage site. Homozygous Fib(AEK) mice developed to term and exhibited postnatal survival superior to that of fibrinogen-deficient mice. Unlike fibrinogen-deficient mice, platelet-rich plasma from Fib(AEK) mice supported normal platelet aggregation in vitro, highlighting that fibrinogen(AEK) retains the functional capacity to support interactions with platelets. Thrombin failed to release fibrinopeptide-A from fibrinogen(AEK) and failed to induce polymer formation with Fib(AEK) plasma or purified fibrinogen(AEK) in 37°C mixtures regardless of incubation time. Fib(AEK) mice displayed both an absence of fibrin polymer formation following liver injury, as assessed by electron microscopy, and a failure to generate stable occlusive thrombi following FeCl3 injury of carotid arteries. Fib(AEK) mice exhibited a profound impediment in Staphylococcus aureus clearance following intraperitoneal infection similar to fibrinogen-deficient mice, yet Fib(AEK) mice displayed a significant infection dose-dependent survival advantage over fibrinogen-deficient mice following peritonitis challenge. Collectively, these findings establish for the first time that fibrin polymer is the molecular form critical for antimicrobial mechanisms while simultaneously highlighting biologically meaningful contributions and functions of the soluble molecule. PMID:26228483

  12. Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series

    Directory of Open Access Journals (Sweden)

    Pak Jaewoo

    2011-07-01

    Full Text Available Abstract Introduction This is a series of clinical case reports demonstrating that a combination of percutaneously injected autologous adipose-tissue-derived stem cells, hyaluronic acid, platelet rich plasma and calcium chloride may be able to regenerate bones in human osteonecrosis, and with addition of a very low dose of dexamethasone, cartilage in human knee osteoarthritis. Case reports Stem cells were obtained from adipose tissue of abdominal origin by digesting lipoaspirate tissue with collagenase. These stem cells, along with hyaluronic acid, platelet rich plasma and calcium chloride, were injected into the right hip of a 29-year-old Korean woman and a 47-year-old Korean man. They both had a history of right hip osteonecrosis of the femoral head. For cartilage regeneration, a 70-year-old Korean woman and a 79-year-old Korean woman, both with a long history of knee pain due to osteoarthritis, were injected with stem cells along with hyaluronic acid, platelet rich plasma, calcium chloride and a nanogram dose of dexamethasone. Pre-treatment and post-treatment MRI scans, physical therapy, and pain score data were then analyzed. Conclusions The MRI data for all the patients in this series showed significant positive changes. Probable bone formation was clear in the patients with osteonecrosis, and cartilage regeneration in the patients with osteoarthritis. Along with MRI evidence, the measured physical therapy outcomes, subjective pain, and functional status all improved. Autologous mesenchymal stem cell injection, in conjunction with hyaluronic acid, platelet rich plasma and calcium chloride, is a promising minimally invasive therapy for osteonecrosis of femoral head and, with low-dose dexamethasone, for osteoarthritis of human knees.

  13. Autologous bone-marrow mesenchymal cell induced chondrogenesis (MCIC).

    Science.gov (United States)

    Huh, Sung Woo; Shetty, Asode Ananthram; Ahmed, Saif; Lee, Dong Hwan; Kim, Seok Jung

    2016-01-01

    Degenerative and traumatic articular cartilage defects are common, difficult to treat, and progressive lesions that cause significant morbidity in the general population. There have been multiple approaches to treat such lesions, including arthroscopic debridement, microfracture, multiple drilling, osteochondral transplantation and autologous chondrocyte implantation (ACI) that are currently being used in clinical practice. Autologous bone-marrow mesenchymal cell induced chondrogenesis (MCIC) is a single-staged arthroscopic procedure. This method combines a modified microfracture technique with the application of a bone marrow aspirate concentrate (BMAC), hyaluronic acid and fibrin gel to treat articular cartilage defects. We reviewed the current literatures and surgical techniques for mesenchymal cell induced chondrogenesis. PMID:27489409

  14. Treatment of a hip capsular injury in a professional soccer player with platelet-rich plasma and bone marrow aspirate concentrate therapy.

    Science.gov (United States)

    Campbell, Kevin J; Boykin, Robert E; Wijdicks, Coen A; Erik Giphart, J; LaPrade, Robert F; Philippon, Marc J

    2013-07-01

    This report presents a 27-year-old male professional soccer player who developed heterotopic ossification of his hip capsule and gluteus minimus tendon after an arthroscopic hip procedure. After removal of the heterotopic bone, the patient had a symptomatic deficiency of his hip capsule and gluteus minimus tendon. A series of orthobiologic treatments with platelet-rich plasma and bone marrow aspirate concentrate improved the patient's pain and strength as well as the morphologic appearance of the hip capsule and gluteus minimus tendon on magnetic resonance imaging. A series of motion analyses demonstrated significant improvement in his stance-leg ground reaction force and hip abduction, as well as linear foot velocity at ball strike and maximum hip flexion following ball strike in his kicking leg. Level of evidence IV. PMID:23052123

  15. Plasma rico em plaquetas de coelhos: introdução a um modelo animal experimental Platelet-rich plasma in rabbits: introduction of one experimental animal model

    Directory of Open Access Journals (Sweden)

    Marco Antonio de Oliveira-Filho

    2008-12-01

    Full Text Available RACIONAL: Muitas dúvidas ainda permanecem no que se refere às ações dos fatores de crescimento e do plasma rico em plaquetas sobre o mecanismo de reparação tissular. Há necessidade de serem esclarecidos pontos controversos ainda existentes. OBJETIVO: Obter o plasma rico em plaquetas em coelhos através de um método simplificado e ao mesmo tempo adequado, introduzindo um modelo experimental que possa ser utilizado em estudos posteriores. MÉTODOS: Foram utilizados 25 coelhas da raça Nova Zelândia e sem doenças prévias. Quinze mL de sangue de cada animal foi coletado, sendo 10 mL submetidos à dupla centrifugação. Para comprovar a efetividade do método proposto realizou-se contagem mecânica do sangue, bem como do produto final. RESULTADO: Obteve-se uma concentração média de plaquetas no plasma rico em plaquetas 687% maior que a contagem inicial observada no sangue venoso periférico. Para as variáveis: contagem inicial de plaquetas, contagem de plaquetas no plasma rico em plaquetas e enriquecimento, foram obtidos os limites de 95% de confiança para suas médias, sendo que, no que se refere ao percentual de enriquecimento, existe 95% de chance de que o intervalo de (530-844 contenha a média real de enriquecimento de plaquetas. CONCLUSÃO: O método simplificado utilizado permite a obtenção de plasma rico em plaquetas adequado permitindo seu uso em estudos dos fatores de crescimento nos mecanismos de reparação tecidual.BACKGROUND: Multiple uncertainties still exist about the action of the growth factors and the platelet-rich plasma on the mechanism of repair. AIM: To obtain the platelet-rich plasma in rabbits through a simplified and suitable method, creating an experimental model. METHODS: Twenty-five female New Zealand rabbits without previous diseases were used. Fifteen mL of blood of each rabbit was collected and 10 mL of the collected blood were twice centrifugated. To check the effectiveness of the proposed method

  16. Efficacy of Platelet-Rich-Plasma (PRP and Highly Purified Bovine Xenograft (Laddec® Combination in Bone Regeneration after Cyst Enucleation: Radiological and Histological Evaluation

    Directory of Open Access Journals (Sweden)

    Sabrina Pappalardo

    2013-10-01

    Full Text Available Objectives: The purpose of the present study was to evaluate the efficacy of adding platelet-rich plasma (PRP to a new highly purified bovine allograft (Laddec® in the bone regeneration of cystic bony defects augmented following cystectomy.Material and Methods: Study sample included 20 patients undergoing cystectomy in which the bone defect was filled with PRP and Laddec®. All patients were examined with periapical radiographs before operation and at follow-up. After 3 months, at re-entry surgery for implant placement, bone core was taken for histological and histomorphometric analysis.Results: The postoperative successive radiographs showed a good regeneration of bone in the height of bony defects with application of PRP to bone graft. By the first postoperative month, about 48% of the defect was filled, which gradually increased in each month and showed about 90% of defect-fill by 6 months. Histological and histomorphometric analysis, showed a significant presence of bone tissue and vessels, with newly formed bone in contact with anorganic bone particles. The mean volume of vital bone was 68 ± 1.6% and the mean percentage of vital bone was 48 ± 2.4%. The mean percentage of inorganic particles in tissues was 20 ± 1.2% of the total volume. All the samples analyzed did not evidence the presence of inflammatory cells.Conclusions: The results of this study showed how the use of Laddec® in association with platelet-rich plasma allows bone regeneration and has a potential for routine clinical use for regeneration of cystic bony defects.

  17. The composite of bone marrow concentrate and PRP as an alternative to autologous bone grafting.

    Directory of Open Access Journals (Sweden)

    Mohssen Hakimi

    Full Text Available One possible alternative to the application of autologous bone grafts represents the use of autologous bone marrow concentrate (BMC. The purpose of our study was to evaluate the potency of autologous platelet-rich plasma (PRP in combination with BMC. In 32 mini-pigs a metaphyseal critical-size defect was surgically created at the proximal tibia. The animals were allocated to four treatment groups of eight animals each (1. BMC+CPG group, 2. BMC+CPG+PRP group, 3. autograft group, 4. CPG group. In the BMC+CPG group the defect was filled with autologous BMC in combination with calcium phosphate granules (CPG, whereas in the BMC+CPG+PRP group the defect was filled with the composite of autologous BMC, CPG and autologous PRP. In the autograft group the defect was filled with autologous cancellous graft, whereas in the CPG group the defect was filled with CPG solely. After 6 weeks radiological and histomorphometrical analysis showed significantly more new bone formation in the BMC+CPG+PRP group compared to the BMC+CPG group and the CPG group. There were no significant differences between the BMC+CPG+PRP group and the autograft group. In the PRP platelets were enriched significantly about 4.7-fold compared to native blood. In BMC the count of mononuclear cells increased significantly (3.5-fold compared to the bone marrow aspirate. This study demonstrates that the composite of BMC+CPG+PRP leads to a significantly higher bone regeneration of critical-size defects at the proximal tibia in mini-pigs than the use of BMC+CPG without PRP. Furthermore, within the limits of the present study the composite BMC+CPG+PRP represents a comparable alternative to autologous bone grafting.

  18. O gel de plasma rico em plaquetas propicia a regeneração da cartilagem articular do joelho de ovelhas Platelet-rich plasma gel promotes regeneration of articular cartilage in knees of sheeps

    Directory of Open Access Journals (Sweden)

    Márcio de Oliveira Carneiro

    2013-04-01

    Full Text Available OBJETIVO: Avaliar a regeneração da cartilagem articular em defeitos osteocondrais do joelho induzidos pelo plasma rico em plaquetas (PRP autógeno. MÉTODOS: Defeitos osteocondrais produzidos no sulco troclear de ambos os joelhos de dez ovelhas foram preenchidos com PRP autógeno à direita e deixados vazios à esquerda. Avaliação macroscópica e histológica foram efetuadas 12 semanas mais tarde. Os resultados foram avaliados por um escore geral de ambas as avaliações macroscópica e histológica comparativamente entre os lados por meio do teste pareado de Wilcoxon. RESULTADOS: o aspecto macroscópico não foi uniforme entre os animais, nem diferiu entre os joelhos direitos e esquerdos (p=0,03125; em nenhum caso o tecido regenerado se nivelou com a cartilagem normal circundante. Ao exame histológico, cartilagem aparentemente normal não foi detectada em nenhum joelho, mas uma cartilagem pouco diferenciada estava presente em sete joelhos direitos e em três joelhos esquerdos. Tecido fibrocartilaginoso estava presente nos joelhos restantes, com diferença significante no escore geral entre os joelhos direitos e esquerdos (p=0,0313. CONCLUSÃO: o PRP como usado neste estudo tem propriedades reparativas da cartilagem articular no joelho de ovelhas, principalmente por estimular a formação de tecido fibrocartilaginoso. Trabalho Experimental.OBJECTIVE: to assess the regeneration of osteochondral defects in the joint cartilage of the knee induced by autologous platelet-rich plasma (pRp. METHODS: osteochondral defects produced in the trochlear groove of both knees of ten sheep; defects of the right knees were filled with autologous pRp and the left knees were left unfilled. macroscopic and microscopic evaluation was carried out 12 week later. the results were evaluated by the total score of both macroscopic and microscopic evaluations comparing the two sides through the wilcoxon paired test. RESULTS: macroscopic appearance was not uniform among

  19. Fibrin clot formation and lysis: basic mechanisms

    DEFF Research Database (Denmark)

    Sidelmann, JJ; Gram, J; Jespersen, J;

    2000-01-01

    The hemostatic balance, introduced more than 40 years ago, addresses the components and reactions involved in fibrin turnover. Fibrin is placed in the core of this delicate balance. Defects in the mechanisms responsible for fibrin turnover might lead to thrombosis or bleeding, and fibrin conseque...

  20. Cytokine profile of autologous platelet-derived eye drops in patients with ocular chronic graft-versus-host disease.

    Science.gov (United States)

    Valentini, C G; Nuzzolo, E R; Orlando, N; Metafuni, E; Bianchi, M; Chiusolo, P; Zini, G; Teofili, L

    2016-02-01

    Ocular chronic GVHD is efficaciously treated with autologous platelet-derived eye drops. We investigated the cytokine content of eye drops produced using a non-gelified lysate obtained from autologous platelet-rich plasma in six patients with ocular GVHD. In both the responding (n = 4) and the resistant (n = 2) patients, the eye drops were significantly enriched with various growth factors, in amounts proportional with the platelet counts. In contrast, chemokine ligand and interleukin levels were similar to those of plasma. The non-responding patients showed the highest levels of chemokine (C-X-C motif) ligand (CXCL)10. These findings provide possible explanations for beneficial or detrimental effects of eye drops. PMID:26383050

  1. Clinical effectiveness in severe knee osteoarthritis after intra-articular platelet-rich plasma therapy in association with hyaluronic acid injection: three case reports

    Science.gov (United States)

    Chen, Szu-Hsuan; Kuan, Ta-Shen; Kao, Mu-Jung; Wu, Wei-Ting; Chou, Li-Wei

    2016-01-01

    Osteoarthritis (OA) is a degenerative disorder resulting from loss of joint cartilage and underlying bone and causes pain and loss of function. The treatment of knee OA is still a challenge because of the poor self-regeneration capacity of cartilage. The nonsurgical interventions include control of the aggravating factor (such as weight control and the use of walking aids), symptomatic treatment (such as acetaminophen or nonsteroidal anti-inflammatory drugs), prolotherapy, and viscosupplementation. However, the combination of platelet-rich plasma (PRP) and hyaluronic acid (HA) has not been widely used because of lack of clinical evidence and several limitations in patients with severe knee OA. Three patients who suffered from knee pain and poor walking endurance were diagnosed with advanced knee OA. They underwent PRP treatment in association with intra-articular HA injection and showed pain relief and functional improvement. The follow-up standard weight-bearing X-ray images of knees also confirmed the improvement and indicated the possibility of regeneration of the articular cartilage. These cases provide clinical and radiographic evidence for a new therapy for advanced knee OA. This treatment strategy of PRP in association with HA injection can offer a chance to treat severe knee OA, rather than immediate surgery, or a chance for those who cannot undergo surgery. It can also postpone the need of arthroplasty and can significantly improve the daily activity function. PMID:27660427

  2. Engineering new bone via a minimally invasive route using human bone marrow-derived stromal cell aggregates, microceramic particles, and human platelet-rich plasma gel.

    Science.gov (United States)

    Chatterjea, Anindita; Yuan, Huipin; Fennema, Eelco; Burer, Ruben; Chatterjea, Supriyo; Garritsen, Henk; Renard, Auke; van Blitterswijk, Clemens A; de Boer, Jan

    2013-02-01

    There is a rise in the popularity of arthroscopic procedures in orthopedics. However, the majority of cell-based bone tissue-engineered constructs (TECs) rely on solid preformed scaffolding materials, which require large incisions and extensive dissections for placement at the defect site. Thus, they are not suitable for minimally invasive techniques. The aim of this study was to develop a clinically relevant, easily moldable, bone TEC, amenable to minimally invasive techniques, using human mesenchymal stromal cells (hMSCs) and calcium phosphate microparticles in combination with an in situ forming platelet-rich plasma gel obtained from human platelets. Most conventional TECs rely on seeding and culturing single-cell suspensions of hMSCs on scaffolds. However, for generating TECs amenable to the minimally invasive approach, it was essential to aggregate the hMSCs in vitro before seeding them on the scaffolds as unaggregated MSCs did not generate any bone. Twenty four hours of in vitro aggregation was determined to be optimal for maintaining cell viability in vitro and bone formation in vivo. Moreover, no statistically significant difference was observed in the amount of bone formed when the TECs were implanted via an open approach or a minimally invasive route. TECs generated using MSCs from three different human donors generated new bone through the minimally invasive route in a reproducible manner, suggesting that these TECs could be a viable alternative to preformed scaffolds employed through an open surgery for treating bone defects.

  3. PLATELET-RICH PLASMA (PRP AND ITS APPLICATION IN THE TREATMENT OF CHRONIC AND HARD-TO-HEAL SKIN WOUNDS. A Review.

    Directory of Open Access Journals (Sweden)

    Tsvetan Sokolov

    2015-12-01

    Full Text Available In the last few years various methods are being applied in the use of platelet-rich plasma (PRP during treatment in different orthopedic disease. They allow improvement of local biological condition and regeneration of different types of tissues. PRP is a modern treatment strategy with worldwide recognition. There is a high concentration of platelet growth factors in small amounts of plasma. PRP and its various forms have become one of the best methods to support the healing process of various tissues. PRP is used in regenerative medicine, because it provides two of three components (growth factors and scaffolds necessary for complete tissue regeneration. The particular reason for the appearance of lesions is important in order to select an appropriate treatment method and technical application. PRP may be used for treatment of various chronic and hard-to-heal cutaneous wounds, especially when standard conventional therapy is not good enough and surgical treatment is not possible. It reduces the duration, cost of treatment and the hospital stay. There is reduction of wound pain after starting the treatment, reduced risk of blood-borne disease transmission, wound healing is restored, and local immunity is activated.

  4. Polyethylene Oxide Films Polymerized by Radio Frequency Plasma-Enhanced Chemical Vapour Phase Deposition and Its Adsorption Behaviour of Platelet-Rich Plasma

    Institute of Scientific and Technical Information of China (English)

    HU Wen-Juan; XIE Fen-Yan; CHEN Qiang; WENG Jing

    2008-01-01

    We present polyethylene oxide (PEO) functional films polymerized by rf plasma-enhanced vapour chemical deposition (rf-PECVD) on p-Si (100) surface with precursor ethylene glycol dimethyl ether (EGDME) and diluted Ar in pulsed plasma mode. The influences of discharge parameters on the film properties and compounds are investigated. The film structure is analysed by Fourier transform infrared (FTIR) spectroscopy. The water contact angle measurement and atomic force microscope (AFM) are employed to examine the surface polarity and to detect surface morphology, respectively. It is concluded that the smaller duty cycle in pulsed plasma mode contributes to the rich C-O-C (EO) group on the surfaces. As an application, the adsorption behaviour of platelet-rich plasma on plasma polymerization films performed in-vitro is explored. The shapes of attached cells are studied in detail by an optic invert microscope, which clarifies that high-density C-O-C groups on surfaces are responsible for non-fouling adsorption behaviour of the PEO films.

  5. Bone Tissue Engineering with Adipose-Derived Stem Cells in Bioactive Composites of Laser-Sintered Porous Polycaprolactone Scaffolds and Platelet-Rich Plasma

    Directory of Open Access Journals (Sweden)

    Han-Tsung Liao

    2013-10-01

    Full Text Available Three-dimensional porous polycaprolactone (PCL scaffolds with consistent inter-pore channels, 83% porosity and 300–400 μm pore size were fabricated via selective laser sintering. The PCL scaffold was combined with platelet-rich plasma (PRP to form a bioactive composite and studied for potential application in bone tissue engineering using porcine adipose-derived stem cells (PASCs. The PCL/PRP/PASCs construct showed enhanced cell seeding efficiency and synergistically increased the differentiation capability of PASCs in osteogenic medium toward the osteoblast lineage, judging from elevated alkaline phosphatase activity and up-regulated osteogenic genes expression. For in vivo study, a 3 cm × 3 cm mandible defect was created in pigs and reconstructed by implanting acellular PCL scaffolds or PCL/PRP/PASCs constructs. Both groups showed new bone formation, however, the new bone volume was 5.1 times higher for PCL/PRP/PASCs 6 months post-operation. The bone density was less and loose in the acellular PCL group and the Young’s modulus was only 29% of normal bone. In contrast, continued and compact bone formation was found in PCL/PRP/PASCs and the Young’s modulus was 81% that of normal bone. Masson’s trichrome stain, immunohistochemical analysis of osteocalcin and collagen type I also confirmed new bone formation.

  6. Electrospun silk fibroin/poly (L-lactide-ε-caplacton) graft with platelet-rich growth factor for inducing smooth muscle cell growth and infiltration.

    Science.gov (United States)

    Yin, Anlin; Bowlin, Gary L; Luo, Rifang; Zhang, Xingdong; Wang, Yunbing; Mo, Xiumei

    2016-12-01

    The construction of a smooth muscle layer for blood vessel through electrospinning method plays a key role in vascular tissue engineering. However, smooth muscle cells (SMCs) penetration into the electrospun graft to form a smooth muscle layer is limited due to the dense packing of fibers and lack of inducing factors. In this paper, silk fibroin/poly (L-lactide-ε-caplacton) (SF/PLLA-CL) vascular graft loaded with platelet-rich growth factor (PRGF) was fabricated by electrospinning. The in vitro results showed that SMCs cultured in the graft grew fast, and the incorporation of PRGF could induce deeper SMCs infiltrating compared to the SF/PLLA-CL graft alone. Mechanical properties measurement showed that PRGF-incorporated graft had proper tensile stress, suture retention strength, burst pressure and compliance which could match the demand of native blood vessel. The success in the fabrication of PRGF-incorporated SF/PLLA-CL graft to induce fast SMCs growth and their strong penetration into graft has important application for tissue-engineered blood vessels. PMID:27482466

  7. Multi-Composite Bioactive Osteogenic Sponges Featuring Mesenchymal Stem Cells, Platelet-Rich Plasma, Nanoporous Silicon Enclosures, and Peptide Amphiphiles for Rapid Bone Regeneration

    Directory of Open Access Journals (Sweden)

    Dongmei Fan

    2011-06-01

    Full Text Available A novel bioactive sponge was created with a composite of type I collagen sponges or porous poly(e-caprolactone (PCL scaffolds, platelet-rich plasma (PRP, BMP2-loaded nanoporous silicon enclosure (NSE microparticles, mineralizing peptide amphiphiles (PA, and mesenchymal stem cells (MSC. Primary MSC from cortical bone (CB  tissue proved to form more and larger colony units, as well as produce more mineral matrix under osteogenic differentiation, than MSC from bone marrow (BM. Coating pre-treatments were optimized for maximum cell adhesion and mineralization, while a PRP-based gel carrier was created to efficiently deliver and retain MSC and  microparticles within a porous scaffold while simultaneously promoting cell recruitment, proliferation, and angiogenesis. Components and composite sponges were evaluated for osteogenic differentiation in vitro. Osteogenic sponges were loaded with MSC, PRP, PA, and NSE and implanted subcutaneously in rats to evaluate the formation of bone tissue and angiogenesis in vivo. It was found that the combination of a collagen sponge with CB MSC, PRP, PA, and the BMP2-releasing NSE formed the most bone and was most vascularized by four weeks compared to analogous composites featuring BM MSC or PCL or lacking PRP, PA, and NSE. This study indicates that CB MSC should be considered as an alternative to marrow as a source of stem cells, while the PRP-PA cell and microparticle delivery system may be utilized for diverse tissue engineering applications.

  8. Effectiveness of platelet-rich plasma as an adjunctive material to bone graft: a systematic review and meta-analysis of randomized controlled clinical trials.

    Science.gov (United States)

    Pocaterra, A; Caruso, S; Bernardi, S; Scagnoli, L; Continenza, M A; Gatto, R

    2016-08-01

    The use of platelet-rich plasma (PRP) has become a strategic therapy in tissue regeneration medicine. PRP represents a good source of growth factors. Due to this property, it has been considered a reliable adjunctive material in bone augmentation procedures, such as the sinus lift technique. The aim of this review was to assess the scientific evidence on the effectiveness of PRP as an adjunctive material in the sinus floor elevation technique. The following databases were searched for relevant published studies: Medline, Cochrane Central Register of Controlled Trials, Cochrane Database of Systematic Reviews, CINAHL, Science Direct, ISI Web of Knowledge, and SCOPUS. Only randomized controlled clinical trials comparing a group receiving PRP as an adjunctive material to a control group without PRP, involving adult human subjects (age >18 years) with no systemic disease, were included. Of the studies identified, only one reported a significant difference in bone augmentation in favour of the adjunctive use of PRP, while four studies did not find any significant difference. None of the studies included reported a significant difference in the implant survival rate. Further randomized clinical trials are needed to clarify the effectiveness of adjunctive PRP. PMID:26987695

  9. Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro.

    Science.gov (United States)

    do Amaral, Ronaldo José Farias Corrêa; da Silva, Nemias Pereira; Haddad, Natália Ferreira; Lopes, Luana Siqueira; Ferreira, Fábio Dias; Filho, Ricardo Bastos; Cappelletti, Paola Alejandra; de Mello, Wallace; Cordeiro-Spinetti, Eric; Balduino, Alex

    2016-01-01

    There are promising results in the use of platelet-rich plasma (PRP) for musculoskeletal tissue repair. However, the variability in the methodology for its obtaining may cause different and opposing findings in the literature. Particularly, the choice of the anticoagulant is the first definition to be made. In this work, blood was collected with sodium citrate (SC), ethylenediaminetetraacetic acid (EDTA), or anticoagulant citrate dextrose (ACD) solution A, as anticoagulants, prior to PRP obtaining. Hematological analysis and growth factors release quantification were performed, and the effects on mesenchymal stromal cell (MSC) culture, such as cytotoxicity and cell proliferation (evaluated by MTT method) and gene expression, were evaluated. The use of EDTA resulted in higher platelet yield in whole blood; however, it induced an increase in the mean platelet volume (MPV) following the blood centrifugation steps for PRP obtaining. The use of SC and ACD resulted in higher induction of MSC proliferation. On the other hand, PRP obtained in SC presented the higher platelet recovery after the blood first centrifugation step and a minimal change in MSC gene expression. Therefore, we suggest the use of SC as the anticoagulant for PRP obtaining. PMID:27340410

  10. Investigation of modified platelet-rich plasma (mPRP) in promoting the proliferation and differentiation of dental pulp stem cells from deciduous teeth.

    Science.gov (United States)

    Wen, J; Li, H T; Li, S H; Li, X; Duan, J M

    2016-01-01

    Stem cells from human exfoliated deciduous teeth (SHEDs) have great potential to treat various dental-related diseases in regenerative medicine. They are usually maintained with 10% fetal bovine serum (FBS) in vitro. Modified platelet-rich plasma (mPRP) would be a safe alternative to 10% FBS during SHEDs culture. Therefore, our study aimed to compare the proliferation and differentiation of SHEDs cultured in mPRP and FBS medium to explore an optimal concentration of mPRP for SHEDs maintenance. Platelets were harvested by automatic blood cell analyzer and activated by repeated liquid nitrogen freezing and thawing. The platelet-related cytokines were examined and analyzed by ELISA. SHEDs were extracted and cultured with different concentrations of mPRP or 10% FBS medium. Alkaline phosphatase (ALP) activity was measured. Mineralization factors, RUNX2 and OCN, were measured by real-time PCR. SHEDs were characterized with mesenchymal stem cells (MSCs) markers including vimentin, CD44, and CD105. mPRP at different concentrations (2, 5, 10, and 20%) enhanced the growth of SHEDs. Moreover, mPRP significantly stimulated ALP activity and promoted expression of RUNX2 and OCN compared with 10% FBS. mPRP could efficiently facilitate proliferation and differentiation of SHEDs, and 2% mPRP would be an optimal substitute for 10% FBS during SHEDs expansion and differentiation in clinical scale manufacturing. PMID:27599200

  11. Platelet-rich plasma, especially when combined with a TGF-β inhibitor promotes proliferation, viability and myogenic differentiation of myoblasts in vitro.

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    Robi Kelc

    Full Text Available Regeneration of skeletal muscle after injury is limited by scar formation, slow healing time and a high recurrence rate. A therapy based on platelet-rich plasma (PRP has become a promising lead for tendon and ligament injuries in recent years, however concerns have been raised that PRP-derived TGF-β could contribute to fibrotic remodelling in skeletal muscle after injury. Due to the lack of scientific grounds for a PRP -based muscle regeneration therapy, we have designed a study using human myogenic progenitors and evaluated the potential of PRP alone and in combination with decorin (a TGF-β inhibitor, to alter myoblast proliferation, metabolic activity, cytokine profile and expression of myogenic regulatory factors (MRFs. Advanced imaging multicolor single-cell analysis enabled us to create a valuable picture on the ratio of quiescent, activated and terminally committed myoblasts in treated versus control cell populations. Finally high-resolution confocal microscopy validated the potential of PRP and decorin to stimulate the formation of polynucleated myotubules. PRP was shown to down-regulate fibrotic cytokines, increase cell viability and proliferation, enhance the expression of MRFs, and contribute to a significant myogenic shift during differentiation. When combined with decorin further synergistc effects were identified. These results suggest that PRP could not only prevent fibrosis but could also stimulate muscle commitment, especially when combined with a TGF-β inhibitor.

  12. Plasma rico em plaquetas no reparo artroscópico das roturas completas do manguito rotador Platelet-rich plasma in arthroscopic repairs of complete tears of the rotator cuff

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    Eduardo Angeli Malavolta

    2012-01-01

    Full Text Available OBJETIVO: Avaliar os resultados funcionais e o índice de rerrotura do reparo do manguito rotador por via artroscópica associado ao uso do PRP. MÉTODOS: Série de casos prospectiva, avaliando os resultados do reparo artroscópico do manguito rotador em fileira simples associada ao uso do PRP. Foram incluídas apenas roturas isoladas do supraespinal, com retração inferior a 3cm. O PRP utilizado foi obtido pelo método de aférese, e aplicado em sua forma ativada, com a adição de trombina autóloga, na consistência líquida. A avaliação pós-operatória foi realizada de maneira padronizada, aos 12 meses de seguimento. Foram utilizadas as escalas de Constant-Murley, UCLA e EVA, além da análise da incidência de rerroturas através da ressonância magnética. RESULTADOS: Foram avaliados 14 pacientes (14 ombros. A escala de Constant-Murley evoluiu em média de 45,64 ± 12,29 no pré-operatório para 80,78 ± 13,22 no pós-operatório (p OBJECTIVE: To evaluate shoulder functional results and the re tear rate of arthroscopic repair of the rotator cuff augmented with platelet-rich plasma (PRP. METHODS: Prospective case series with single-row arthroscopic repair of the rotator cuff augmented with PRP. Only cases of isolated supraspinatus tears with retraction of less than 3 cm were included in this series. The PRP used was obtained by apheresis. It was applied on liquid consistency in its activated form, with the addition of autologous thrombin. Patients were evaluated after 12 months of the surgical procedure. The Constant-Murley, UCLA and VAS scales were used, and the retear rate was assessed using magnetic resonance imaging (MRI. RESULTS: Fourteen patients were evaluated (14 shoulders. The mean Constant-Murley score was 45.64 ± 12.29 before the operation and evolved to 80.78 ± 13.22 after the operation (p < 0.001. The UCLA score increased from 13.78 ± 5.66 to 31.43 ± 3.9 (p < 0.001. The patients' pain level decreased from a median of 7

  13. Quality assessment of platelet concentrates prepared by platelet rich plasma-platelet concentrate, buffy coat poor-platelet concentrate (BC-PC and apheresis-PC methods

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    Singh Ravindra

    2009-01-01

    Full Text Available Background: Platelet rich plasma-platelet concentrate (PRP-PC, buffy coat poor-platelet concentrate (BC-PC, and apheresis-PC were prepared and their quality parameters were assessed. Study Design: In this study, the following platelet products were prepared: from random donor platelets (i platelet rich plasma - platelet concentrate (PRP-PC, and (ii buffy coat poor- platelet concentrate (BC-PC and (iii single donor platelets (apheresis-PC by different methods. Their quality was assessed using the following parameters: swirling, volume of the platelet concentrate, platelet count, WBC count and pH. Results: A total of 146 platelet concentrates (64 of PRP-PC, 62 of BC-PC and 20 of apheresis-PC were enrolled in this study. The mean volume of PRP-PC, BC-PC and apheresis-PC was 62.30±22.68 ml, 68.81±22.95 ml and 214.05±9.91 ml and ranged from 22-135 ml, 32-133 ml and 200-251 ml respectively. The mean platelet count of PRP-PC, BC-PC and apheresis-PC was 7.6±2.97 x 1010/unit, 7.3±2.98 x 1010/unit and 4.13±1.32 x 1011/unit and ranged from 3.2-16.2 x 1010/unit, 0.6-16.4 x 1010/unit and 1.22-8.9 x 1011/unit respectively. The mean WBC count in PRP-PC (n = 10, BC-PC (n = 10 and apheresis-PC (n = 6 units was 4.05±0.48 x 107/unit, 2.08±0.39 x 107/unit and 4.8±0.8 x 106/unit and ranged from 3.4 -4.77 x 107/unit, 1.6-2.7 x 107/unit and 3.2 - 5.2 x 106/unit respectively. A total of 26 units were analyzed for pH changes. Out of these units, 10 each were PRP-PC and BC-PC and 6 units were apheresis-PC. Their mean pH was 6.7±0.26 (mean±SD and ranged from 6.5 - 7.0 and no difference was observed among all three types of platelet concentrate. Conclusion: PRP-PC and BC-PC units were comparable in terms of swirling, platelet count per unit and pH. As expected, we found WBC contamination to be less in BC-PC than PRP-PC units. Variation in volume was more in BC-PC than PRP-PC units and this suggests that further standardization is required for preparation of BC

  14. Platelet-rich plasma and adipose-derived mesenchymal stem cells for regenerative medicine-associated treatments in bottlenose dolphins (Tursiops truncatus.

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    Richard J Griffeth

    Full Text Available Dolphins exhibit an extraordinary capacity to heal deep soft tissue injuries. Nevertheless, accelerated wound healing in wild or captive dolphins would minimize infection and other side effects associated with open wounds in marine animals. Here, we propose the use of a biological-based therapy for wound healing in dolphins by the application of platelet-rich plasma (PRP. Blood samples were collected from 9 different dolphins and a specific and simple protocol which concentrates platelets greater than two times that of whole blood was developed. As opposed to a commonly employed human protocol for PRP preparation, a single centrifugation for 3 minutes at 900 rpm resulted in the best condition for the concentration of dolphin platelets. By FACS analysis, dolphin platelets showed reactivity to platelet cell-surface marker CD41. Analysis by electron microscopy revealed that dolphin platelets were larger in size than human platelets. These findings may explain the need to reduce the duration and speed of centrifugation of whole blood from dolphins to obtain a 2-fold increase and maintain proper morphology of the platelets. For the first time, levels of several growth factors from activated dolphin platelets were quantified. Compared to humans, concentrations of PDGF-BB were not different, while TGFβ and VEGF-A were significantly lower in dolphins. Additionally, adipose tissue was obtained from cadaveric dolphins found along the Spanish Mediterranean coast, and adipose-derived mesenchymal stem cells (ASCs were successfully isolated, amplified, and characterized. When dolphin ASCs were treated with 2.5 or 5% dolphin PRP they exhibited significant increased proliferation and improved phagocytotic activity, indicating that in culture, PRP may improve the regenerative capacity of ASCs. Taken together, we show an effective and well-defined protocol for efficient PRP isolation. This protocol alone or in combination with ASCs, may constitute the basis of a

  15. A Pilot Study Evaluating the Effectiveness of Platelet-Rich Plasma Therapy for Treating Degenerative Tendinopathies: A Randomized Control Trial with Synchronous Observational Cohort.

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    Marni Wesner

    Full Text Available This pilot study aimed to inform future research evaluating the effectiveness of Platelet Rich Plasma (PRP injection for tendinopathy.Randomized control trial (RCT and synchronous observational cohort studies. For the RCT, consecutive consenting patients treated at an academic sports medicine clinic were randomly assigned to either a PRP or placebo control group.The Glen Sather Sport Medicine Clinic, Edmonton, Canada.The RCT included 9 participants with rotator cuff tendinopathy. The cohort study included 178 participants with a variety of tendinopathies.Patients receiving PRP were injected with 4 ml of platelets into the supraspinatus and/or infraspinatus, while patients in the placebo group were injected with 4 ml of saline. All participants undertook a 3-month standardized, home-based, daily exercise program.Participants in the RCT were re-evaluated 3, and 6 months post-injection. Change scores before and after injection on pain, disability and MRI-documented pathology outcomes were compared. In the cohort study, pain and disability were measured at 1, 2 and 3 months post-injection.For the RCT, 7 participants received PRP and 2 received placebo injections. Patients receiving PRP reported clinically important improvements in pain (>1.5/10 on VAS, disability (>15 point DASH change, and tendon pathology while those receiving placebo injections did not. In the observational cohort, statistically and clinically significant improvements in pain and disability were observed.This pilot study provides information for planning future studies of PRP effectiveness. Preliminary results indicate intratendinous, ultrasound-guided PRP injection may lead to improvements in pain, function, and MRI-documented tendon pathology.Controlled-Trials.com ISRCTN68341698.

  16. Platelet-rich plasma and adipose-derived mesenchymal stem cells for regenerative medicine-associated treatments in bottlenose dolphins (Tursiops truncatus).

    Science.gov (United States)

    Griffeth, Richard J; García-Párraga, Daniel; Mellado-López, Maravillas; Crespo-Picazo, Jose Luis; Soriano-Navarro, Mario; Martinez-Romero, Alicia; Moreno-Manzano, Victoria

    2014-01-01

    Dolphins exhibit an extraordinary capacity to heal deep soft tissue injuries. Nevertheless, accelerated wound healing in wild or captive dolphins would minimize infection and other side effects associated with open wounds in marine animals. Here, we propose the use of a biological-based therapy for wound healing in dolphins by the application of platelet-rich plasma (PRP). Blood samples were collected from 9 different dolphins and a specific and simple protocol which concentrates platelets greater than two times that of whole blood was developed. As opposed to a commonly employed human protocol for PRP preparation, a single centrifugation for 3 minutes at 900 rpm resulted in the best condition for the concentration of dolphin platelets. By FACS analysis, dolphin platelets showed reactivity to platelet cell-surface marker CD41. Analysis by electron microscopy revealed that dolphin platelets were larger in size than human platelets. These findings may explain the need to reduce the duration and speed of centrifugation of whole blood from dolphins to obtain a 2-fold increase and maintain proper morphology of the platelets. For the first time, levels of several growth factors from activated dolphin platelets were quantified. Compared to humans, concentrations of PDGF-BB were not different, while TGFβ and VEGF-A were significantly lower in dolphins. Additionally, adipose tissue was obtained from cadaveric dolphins found along the Spanish Mediterranean coast, and adipose-derived mesenchymal stem cells (ASCs) were successfully isolated, amplified, and characterized. When dolphin ASCs were treated with 2.5 or 5% dolphin PRP they exhibited significant increased proliferation and improved phagocytotic activity, indicating that in culture, PRP may improve the regenerative capacity of ASCs. Taken together, we show an effective and well-defined protocol for efficient PRP isolation. This protocol alone or in combination with ASCs, may constitute the basis of a biological

  17. Platelet-rich plasma and skeletal muscle healing: a molecular analysis of the early phases of the regeneration process in an experimental animal model.

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    Ivan Dimauro

    Full Text Available Platelet-rich plasma (PRP has received increasing interest in applied medicine, being widely used in clinical practice with the aim of stimulating tissue healing. Despite the reported clinical success, there is still a lack of knowledge when considering the biological mechanisms at the base of the activity of PRP during the process of muscle healing. The aim of the present study was to verify whether the local delivery of PRP modulates specific molecular events involved in the early stages of the muscle regeneration process. The right flexor sublimis muscle of anesthetized Wistar rats was mechanically injured and either treated with PRP or received no treatment. At day 2 and 5 after surgery, the animals were sacrificed and the muscle samples evaluated at molecular levels. PRP treatment increased significantly the mRNA level of the pro-inflammatory cytokines IL-1β, and TGF-β1. This phenomenon induced an increased expression at mRNA and/or protein levels of several myogenic regulatory factors such as MyoD1, Myf5 and Pax7, as well as the muscular isoform of insulin-like growth factor1 (IGF-1Eb. No effect was detected with respect to VEGF-A expression. In addition, PRP application modulated the expression of miR-133a together with its known target serum response factor (SRF; increased the phosphorylation of αB-cristallin, with a significant improvement in several apoptotic parameters (NF-κB-p65 and caspase 3, indexes of augmented cell survival. The results of the present study indicates that the effect of PRP in skeletal muscle injury repair is due both to the modulation of the molecular mediators of the inflammatory and myogenic pathways, and to the control of secondary pathways such as those regulated by myomiRNAs and heat shock proteins, which contribute to proper and effective tissue regeneration.

  18. Nanosecond pulse electric field activation of platelet-rich plasma reduces myocardial infarct size and improves left ventricular mechanical function in the rabbit heart.

    Science.gov (United States)

    Hargrave, Barbara; Li, Francis

    2012-12-01

    In the current study, we used the novel, nonchemical method of nanosecond pulsed electric fields (nsPEF) to investigate the efficiency of a protocol involving the in vivo treatment of the ischemic and reperfused heart and heart cells in culture with platelet-rich plasma (PRP). Associated with the restoration of blood flow to the ischemic tissue is a phenomenon referred to as "ischemic reperfusion injury." Clinically a type of reperfusion injury occurs during coronary bypass surgery once blood perfusion to the heart is restarted. Although the restoration of oxygen to ischemic myocardial cells is critical for tissue survival, reperfusion causes myocardial oxidative stress, attributable in part to the increased production of reactive oxygen species (ROS). Enhanced ROS production is associated with mitochondrial damage. Adult female New Zealand white rabbits were anesthetized and a left thoracotomy performed to expose the heart. The distal segment of the left anterior descending coronary artery was occluded for 15 minutes and then released so reperfusion of the tissue could occur. PRP (.21 mg/heart) or saline was injected into the ischemic area of the myocardium. Mechanical function of the left ventricle was analyzed using a Millar catheter attached to a Micro-Med Analysis System. H9c2 cells in culture were treated with 1 mL of nsPEF activated PRP (1.05 mg/flask) for 24 hours before analysis for ROS production or mitochondrial depolarization damage). The left ventricle contracted and relaxed faster and infarct size was reduced in hearts treated with PRP compared with saline. ROS production and mitochondrial depolarization were reduced in H9c2 cells treated with PRP and stimulated with hydrogen peroxide. These results provide evidence that nsPEFs can successfully be used to prepare PRP and that the PRP is functional in heart protection possibly by reducing ROS generation and stabilizing the mitochondria of the ischemic/reperfused heart.

  19. CLOSURE OF SMALL CENTRAL PERFORATIONS OF TYMPANIC MEMBRANE WITH GELFILM PATCH AND APPLICATION OF PLATELET RICH PLASMA VERSUS CHEMICAL CAUTERIZATION: A COMPARATIVE STUDY

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    Jeena Kunnathully

    2016-03-01

    Full Text Available BACKGROUND Small central perforation of tympanic membrane is a common finding in patients approaching Otolaryngologists. Even though tympanoplasty can provide a disease free ear and restore hearing loss, most of these cases are either left alone or not surgically treated due to lack of patient compliance. OBJECTIVES In the present study conducted in the Department of Otolaryngology and Head and Neck Surgery, Academy of Medical Sciences, Pariyaram, Kerala, India, we have compared the results of two office procedures for closure of small central perforations. STUDY DESIGN Randomised control trial. METHODS Out of 35 cases with small central perforations of tympanic membrane, 18 were subjected to chemical cauterization with Gelfoam patching and 17 underwent freshening with application of Gelfilm patch and Platelet Rich Plasma (PRP. RESULTS Results were measured in terms of healing of perforation and hearing gain at the end of 3 months. Complete closure was achieved in 54% for chemical cauterization and 53% for Gelfilm patching. The mean hearing gain was 2.17 dB and 2.29 dB respectively. CONCLUSION Smaller size, traumatic aetiology and antero-inferior locations of tympanic membrane perforation showed better results, whereas larger perforations, comorbidities like diabetes mellitus and posteroinferior locations showed poorer results. The complications noted were vertigo, more in chemical cauterization group and otomycosis, more in the freshening and Gelfilm patching group. We thus conclude that all cases of dry small central perforations should be given a trial of either of these cost effective nonsurgical methods before resorting to surgery, depending upon patient compliance.

  20. The role of TGF-β1/Smad2/3 pathway in platelet-rich plasma in retarding intervertebral disc degeneration.

    Science.gov (United States)

    Yang, Huilin; Yuan, Chenxi; Wu, Chunshen; Qian, Jiale; Shi, Qing; Li, Xuefeng; Zhu, Xuesong; Zou, Jun

    2016-08-01

    Recent studies have suggested that platelet-rich plasma (PRP) injections are an effective way to retard intervertebral disc degeneration, but the mechanism of action is unclear. Activated platelets release some growth factors, such as transforming growth factor-β1 (TGF-β1), which positively modulate the extracellular matrix of nucleus pulposus cells. The purpose of this study was to explore the mechanism underlying the PRP-mediated inhibition of intervertebral disc degeneration. In an in vitro study, we found that the proliferation of nucleus pulposus cells was greatly enhanced with 2.5% PRP treatment. The TGF-β1 concentration was much higher after PRP treatment. PRP administration effectively increased the collagen II, aggrecan and sox-9 mRNA levels and decreased collagen X levels. However, Western blotting demonstrated that specifically inhibiting TGF-β1 signalling could significantly prevent nucleus pulpous cellular expression of Smad2/3 and matrix protein. In a rabbit study, magnetic resonance imaging revealed significant recovery signal intensity in the intervertebral discs of the PRP injection group compared with the very low signal intensity in the control groups. Histologically, the PRP plus inhibitor injection group had significantly lower expression levels of Smad2/3 and collagen II than the PRP group. These results demonstrated that a high TGF-β1 content in the platelets retarded disc degeneration in vitro and in vivo. Inhibiting the TGF-β1/Smad2/3 pathway could prevent this recovery by inactivating Smad2/3 and down-regulating the extracellular matrix. Therefore, the TGF-β1/Smad2/3 pathway might play a critical role in the ability of PRP to retard intervertebral disc degeneration.

  1. HMGB1 binds to activated platelets via the receptor for advanced glycation end products and is present in platelet rich human coronary artery thrombi.

    Science.gov (United States)

    Ahrens, Ingo; Chen, Yung-Chih; Topcic, Danijal; Bode, Michael; Haenel, David; Hagemeyer, Christoph E; Seeba, Hannah; Duerschmied, Daniel; Bassler, Nicole; Jandeleit-Dahm, Karin A; Sweet, Matthew J; Agrotis, Alex; Bobik, Alex; Peter, Karlheinz

    2015-11-01

    High mobility group box 1 (HMGB1) acts as both a nuclear protein that regulates gene expression, as well as a pro-inflammatory alarmin that is released from necrotic or activated cells. Recently, HMGB1-expression in human atherosclerotic plaques was identified. Therapeutic blockade of HMGB1 reduced the development of diet-induced atherosclerosis in ApoE knockout mice. Thus, we hypothesised an interaction between HMGB1 and activated platelets. Binding of recombinant HMGB1 to platelets was assessed by flow cytometry. HMGB1 bound to thrombin-activated human platelets (MFI 2.49 vs 25.01, p=0.0079). Blood from wild-type, TLR4 and RAGE knockout mice was used to determine potential HMGB1 receptors on platelets. HMGB1 bound to platelets from wild type C57Bl6 (MFI 2.64 vs 20.3, p 0.05). RAGE expression on human platelets was detected by RT-PCR with mRNA extracted from highly purified platelets and confirmed by Western blot and immunofluorescence microscopy. Platelet activation increased RAGE surface expression (MFI 4.85 vs 6.74, p< 0.05). Expression of HMGB1 in human coronary artery thrombi was demonstrated by immunohistochemistry and revealed high expression levels. Platelets bind HMGB1 upon thrombin-induced activation. Platelet specific expression of RAGE could be detected at the mRNA and protein level and is involved in the binding of HMGB1. Furthermore, platelet activation up-regulates platelet surface expression of RAGE. HMGB1 is highly expressed in platelet-rich human coronary artery thrombi pointing towards a central role for HMGB1 in atherothrombosis, thereby suggesting the possibility of platelet targeted anti-inflammatory therapies for atherothrombosis.

  2. Role of Fibrin Sealants in Liver Surgery

    NARCIS (Netherlands)

    de Boer, Marieke T.; Boonstra, Elizabeth A.; Lisman, Ton; Porte, Robert J.

    2012-01-01

    Background: Fibrin sealants are widely used in liver surgery. The aim of this article is to review the literature on evidence of hemostatic and biliostatic capacities of different fibrin sealants in liver surgery. Methods: In PubMed, a literature search was done with the search terms 'fibrin sealant

  3. Study on restoration of bone defects around dental implants by platelet-rich fibrin%富血小板纤维蛋白修复牙种植体周围骨缺损的研究

    Institute of Scientific and Technical Information of China (English)

    杨全全; 何家才; 杨瑞; 陈镇; 方冬冬

    2012-01-01

    36 dental implants were separately implanted in each side of the mandibule of 6 dogs. At the same time, a 3. 5 mm × 3. 5 mm × 3. 5 mm size bone defect was created in the mesial aspect of each implant. 3 kinds of substitutes were grafted in the bone defects ; β-TCP/PRF compounds, β-TCP and blank control ( do not make any deal). Every 2 dogs were sacrificed at 4, 8 and 12 weeks after the implantation, and gross observation, X-rays and histopathology were examined. The results showed 36 dental implants did not fall off, and no severe complications occurred. So β-TCP/PRF is a better bone repair material with the promotion of new bone formation and the eleva -tion of healing quality.%采用牙种植术将36颗牙种植体植于6只杂种犬双侧下颌缺牙区,同时在种植体近中造3.5 mm×3.5 mm×3.5 mm的骨缺损,然后填入β-TCP/ PRF混合物、β-TCP,另设空白对照组(不做任何处理)于各骨缺损区,分别于种植术后4、8、12周各处死2只犬,行大体、X线及组织学观察各个时期各组骨缺损区的成骨情况.植入的36颗种植体无脱落,无明显不良并发症发生.β-TCP/ PRF混合物是一种良好的骨修复材料,具有促进新骨形成的作用,有利于骨组织的愈合.

  4. 同种异体富血小板血浆可增强糖尿病大鼠合成创面胶原%Allogeneic platelet-rich plasma promotes wound collagen synthesis in diabetic rats

    Institute of Scientific and Technical Information of China (English)

    刘宸; 章宏伟; 徐宁

    2014-01-01

    背景:研究显示,自体富血小板血浆有助于促进糖尿病创面的愈合,但同种异体来源的富血小板血浆对糖尿病创面的作用尚不明确。目的:以链脲佐菌素诱导的糖尿病大鼠创面模型为观察对象,观察同种异体来源的富血小板血浆对糖尿病创面胶原合成的作用。方法:30只SD大鼠,采用链脲佐菌素诱导形成糖尿病模型鼠,随机分成富血小板血浆组和糖尿病组,每组15只。于大鼠背部,形成面积为1 cm2的全层皮肤缺损创面,分别涂以0.1 mL同种异体富血小板血浆或生理盐水,3M贴膜封闭,胶布固定。分别于造模后3,7,14 d时每组各取5只动物观察创面愈合率,并切取创面组织行Masson染色观察胶原分布形态、检测创面组织中羟脯氨酸含量及Ⅰ型、Ⅲ型胶原的mRNA相对表达量。结果与结论:造模后第3,7,14天时富血小板血浆组的创面愈合率均高于糖尿病组(P<0.05)。Masson 染色结果显示富血小板血浆组的创面胶原形成较快、且纤维更粗壮、排列更致密。羟脯氨酸检测结果显示,富血小板血浆组的创面中羟脯氨酸含量明显高于糖尿病组(P <0.01)。RT-PCR检测结果显示术后第3、7、14天时,富血小板血浆组创面内Ⅰ型和Ⅲ型胶原的mRNA表达量均高于糖尿病组(P<0.05)。同时Ⅰ/Ⅲ型胶原比值也较糖尿病组明显增高(P<0.05)。结果表明同种异体来源的富血小板血浆可以有效促进糖尿病创面的愈合,这可能与其增强创面胶原合成的作用有关。%BACKGROUND:Platelet-rich plasma has benefit in diabetic wound healing;however, the effect of al ogeneic platelet-rich plasma is stil unclear. OBJECTIVE:To observe the effect of al ogeneic platelet-rich plasma on col agen synthesis during diabetic wound healing. METHODS:Streptozocin-induced diabetic rats were randomly divided into al ogeneic platelet-rich plasma group and saline control

  5. [Autologous blood transfusion].

    Science.gov (United States)

    Rosencher, N; Conseiller, C

    2001-06-30

    Autologous blood transfusion techniques are the principal means of reducing allogeneic blood exposure. Those techniques were developed in order to prevent the risk of contamination by viruses, mainly HVB, HCV and HIV. However that risk has become so small that all studies show an exorbitant cost/efficiency ratio. Autologous blood transfusion would therefore be of no interest in terms of public health but a recent experimental study suggested a possible transmission of the BSE agent through blood. Until the matter is settled, the precaution principle means we should prefer alternative techniques to allogeneic blood whenever possible, hence a renewed interest in autologous transfusion. PMID:11503506

  6. Space maintenance in autogenous fresh demineralized tooth blocks with platelet-rich plasma for maxillary sinus bone formation: a prospective study.

    Science.gov (United States)

    Kim, Eun-Suk; Kang, Ji-Yeon; Kim, Jae-Jin; Kim, Kyoung-Won; Lee, Eun-Young

    2016-01-01

    This prospective study evaluated the effectiveness of autogenous fresh demineralized tooth block (Auto-FDT block) with platelet-rich plasma (PRP) for maxillary sinus augmentation with simultaneous implant installation. Auto-FDT block with PRP was used in Group 1 (n = 15) and combined graft (allograft and xenograft) powder with PRP was used in Group 2 (n = 15). For up to 2 years after the final prosthesis was installed, clinical and radiographic examinations were performed to evaluate the amount of graft materials, residual alveolar height (RAH), sinus height (SH) after grafting, augmented graft height (AGH) and resorption height (RH). In ten cases, biopsies were harvested for histological and histomorphometric analyses. A total of 59 implants were placed in a severe atrophic posterior maxilla with less than 5 mm of RAH and sinus augmentation. None of the patients developed sinusitis or other complications, such as implant loss. The graft material extracted included one molar (or 2 premolars) in Group 1 and 1.8 cc in Group 2. The radiologic examination revealed the following average between-group difference SH (Group 1, 14.12 ± 1.63 mm vs Group 2, 16.51 ± 1.29 mm) and AGH (Group 1, 11.62 ± 2.22 mm vs Group 2, 13.65 ± 1.35 mm). However, sufficient SH and AGH were observed for the implants in the Auto-FDT block group. Two years after final prosthesis was installed, no between-group difference in the RH was observed (Group 1, 1.23 ± 0.73 mm vs Group 2, 1.77 ± 0.54 mm, P = 0.021). The histomorphometric analysis revealed no between-group difference in the new bone volume (Group 1, 23.13 ± 1.42 % vs Group 2, 24.18 ± 2.19 %, P = 0.548). The results showed that Auto-FDT block with PRP can be used in grafted sinuses for implants with only one extracted molar (or two premolars). Auto-FDT block with PRP promotes new bone formation that is comparable with combined grafts. Auto-FDT block with PRP is as an alternative to bone grafting

  7. The synergistic effect of TiO2 nanoporous modification and platelet-rich plasma treatment on titanium-implant stability in ovariectomized rats

    Directory of Open Access Journals (Sweden)

    Jiang N

    2016-09-01

    Full Text Available Nan Jiang,1,2 Pinggong Du,2 Weidong Qu,2 Lin Li,2 Zhonghao Liu,2 Songsong Zhu1 1State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, 2Yantai City Stomatological Hospital, Yantai, People’s Republic of China Abstract: For several decades, titanium and its alloys have been commonly utilized for endosseous implantable materials, because of their good mechanical properties, chemical resistance, and biocompatibility. But associated low bone mass, wear and loss characteristics, and high coefficients of friction have limited their long-term stable performance, especially in certain abnormal bone-metabolism conditions, such as postmenopausal osteoporosis. In this study, we investigated the effects of platelet-rich plasma (PRP treatment and TiO2 nanoporous modification on the stability of titanium implants in osteoporotic bone. After surface morphology, topographical structure, and chemical changes of implant surface had been detected by scanning electron microscopy (SEM, atomic force microscopy, contact-angle measurement, and X-ray diffraction, we firstly assessed in vivo the effect of PRP treatment on osseointegration of TiO2-modified implants in ovariectomized rats by microcomputed tomography examinations, histology, biomechanical testing, and SEM observation. Meanwhile, the potential molecular mechanism involved in peri-implant osseous enhancement was also determined by quantitative real-time polymerase chain reaction. The results showed that this TiO2-modified surface was able to lead to improve bone implant contact, while PRP treatment was able to increase the implant surrounding bone mass. The synergistic effect of both was able to enhance the terminal force of implants drastically in biomechanical testing. Compared with surface modification, PRP treatment promoted earlier osteogenesis with increased expression of the RUNX2 and COL1 genes and

  8. In vitro study of the role of thrombin in platelet rich plasma (PRP) preparation: utility for gel formation and impact in growth factors release

    OpenAIRE

    Huber, Stephany Cares; Cunha Júnior, José Luiz Rosenberis; Montalvão, Silmara; da Silva, Letícia Queiroz; Paffaro, Aline Urban; da Silva, Francesca Aparecida Ramos; Rodrigues, Bruno Lima; Lana, José Fabio Santos Duarte; Annichino-Bizzacchi, Joyce Maria

    2016-01-01

    Introduction: The use of PRP has been studied for different fields, with promising results in regenerative medicine. Until now, there is no study in the literature evaluating thrombin levels in serum, used as autologous thrombin preparation. Therefore, in the present study we evaluated the role played by different thrombin concentrations in PRP and the impact in the release of growth factors. Also, different activators for PRP gel formation were evaluated. Methods: Thrombin levels were measur...

  9. 复合富含血小板血浆联合牛骨形态发生蛋白组织工程骨修复兔桡骨缺损%Effects of platelet-rich plasma in combination with bBMP on bone healing of radius defects in rabbits

    Institute of Scientific and Technical Information of China (English)

    李波; 陈清汉

    2010-01-01

    目的 研究自体富含血小板血浆(PRP)和牛骨形态发生蛋白(bBMP)对兔桡骨缺损区骨组织代谢的影响和促进骨缺损修复的能力.方法 成年新西兰白兔36只,随机分为A、B、C三组,每组12只.A组取右侧桡骨中段2.0 cm节段性骨缺损处植入复合型组织工程骨(PRP+FG+bBMP);B、C两组右侧桡骨缺损处分别植入复合材料(PRP+FG)和复合材料(bBMP+FG).分别于术后4、8、12周各取4只兔子进行计算机图像分析、X线片和组织学切片检查.结果 A组动物实验侧新生骨在骨缺损区所占的面积百分比在4、8、12周时明显高于B、C两组(P<0.05);B组动物新生骨在骨缺损区所占的面积百分比在术后8周、12周均多于C组(P<0.05).结论 PRP与bBMP单独或复合应用均能促进桡骨骨缺损处新骨的生长,但三者联合应用促进骨缺损处新骨的形成效果更明显.%Objective An animal study was carried out to investigate the influence of platelet-rich plasma(PRP) and bovine bone morphogenetic proteins( bBMP)on the bone regeneration of radius defects in rabbits. Methods Thirty-six New Zealand white rabbits were divided randomly into 3 groups: A,B and C. All animals underwent general anesthesia and a 20 mm defects were made in the right radius bone. Implantation of bBMP and PRP with fibrin glue was done in group A,PRP with fibrin glue in group B,bBMP with fibrin glue in group C respectively. The animals were killed after 4,8 and 12 weeks. The specimens were observed by computer graphic analysis, X-ray and histological examination. The results were compared between the three groups. Results At 4,8 and 12 weeks after surgery, area ratio of newly-formed bone on the surface area of radius defects in group A was significantly higher than that group B and C. Area ratio of newly-formed bone on the radius in group B was significantly higher than that on the radius in group C at 8 to 12 weeks after surgery. Conclusions This study shows that the

  10. Autologous blood donation

    OpenAIRE

    Goodnough, Lawrence T

    2004-01-01

    Although preoperative autologous blood donation is employed in elective surgery, this is declining because of the increasingly safe allogeneic blood supply. However, it continues to be used because of the public's perception of allogeneic blood risks and increasing blood shortages. Patients may donate a unit of blood (450 ± 45 ml) as often as twice weekly, up to 72 hours before surgery. Preoperative autologous blood is most beneficial in procedures that cause significant blood loss. It has be...

  11. Teknik Lem Fibrin Otologus pada Cangkok Konjungtiva Bulbi Mata Kelinci

    Directory of Open Access Journals (Sweden)

    Sutarya Enus

    2009-12-01

    Full Text Available Commercial Fibrin Glue (CFG as a biomaterial adhesive from fresh frozen plasma donor and bovine has been tested having various superiorities compared to suturing in bulbar conjunctiva grafting of pterygium surgery. Due to CFG has not been available in Indonesia led the author to make an Autologous Fibrin Glue (AFG directly from patient's own blood. The study aim was to investigate the effectiveness of AFG attachment. This study was an animal experimental, conducted in 24 New Zealand White rabbits divided into AFG group and suturing group of 12 rabbits each, held in Laboratory of Bio Farma, Bandung, from September to December 2006. The assessment included duration (in minute of AFG compared to suturing techniques, also at first day and one week post grafting attachments in bulbar conjunctiva grafting. The statistical analysis used Mann-Whitney and Wilcoxon rank test. In time duration of surgical treatment, AFG technique was significantly shorter than suturing technique (p=0.0001, while first day and one week post grafting AFG attachment showed significantly greater than those of suturing attachment (p=0.0001. In the meantime, first day and one week post grafting AFG attachment was steady state, whereas in suturing technique was different (p=0.0174. In conclusion, AFG technique is more effective than suturing technique in bulbar conjunctiva grafting because of shorter surgical duration, excellent and stable graft attachment.

  12. Effect of Intraoperative Platelet-Rich-Plasma Treatment on Post Operative Donor Site Knee Pain in Patellar Tendon Autograft ACL Reconstruction

    Science.gov (United States)

    Walters, Brian L.; Hobart, Sarah; Porter, David; Hogan, Daniel E.; McHugh, Malachy P.; Bedford, Benjamin B.; Nicholas, Stephen J.; Klein, Devon; Harousseau, Kendall

    2016-01-01

    Objectives: Donor site morbidity in the form of anterior knee pain is a frequent complication after bone-patellar tendon-bone (BPTB) autograft ACL reconstruction. The purpose of this Level I study was to examine the effect of the intraoperative administration of platelet-rich plasma on post operative knee pain and patellar defect healing. Methods: Fifty-nine patients (29±12 y/o) undergoing BPTB ACL reconstruction and eligible to enter the study, were randomized to the treatment (PRP; n=31) or non treatment (sham n=28) arms of the study just prior to surgery. In either case, 10 cc of venous blood was drawn prior to the induction of anesthesia and either discarded (sham) or processed (PRP) for preparation of a PRP gel to be later mixed with donor site bone chips and inserted into the patellar defect. At 12 weeks and 6 months after surgery, patients completed IKDC forms and VAS pain scores for ADLs and kneeling (0-10 scale). Healing indices at the donor site were assessed by MRI at 6 months and included the following measurements taken from axial sequences: AP tendon dimensions at the level of the superior tibial cortex, roof of the intercondylar notch and width at the largest patella graft deficit. Mixed model ANOVA was used to assess the effect of PRP on patient symptoms and MRI indices of donor site healing. The primary dependent variable was VAS kneeling pain. It was estimated that with 25 patients per group there would be 80% power to detect a 1.5-point difference in kneeling pain between treatments at Ppain was deemed to represent a clinically relevant difference. Results: VAS Kneeling Pain at 12 weeks tended to be lower in the PRP versus placebo group (4.5±3.6 vs. 6.2±2.4, P=0.051) but no difference was apparent at 6 months (3.7±3.2 vs. 4.4±2.9, P=0.41). Kneeling pain decreased from 12 weeks to 6 months (PPain with ADLs was not different between treatment groups at 12 weeks (PRP 2.0±2.3 vs. Placebo 2.8±1.8, P=0.16) or 6 months (1.5±1.9 vs. 1.7±2.1, P=0

  13. Plasma rico em plaquetas para reparação de falhas ósseas em cães Platelet-rich plasma for canine bone restoration

    Directory of Open Access Journals (Sweden)

    Anna Laeticia Trindade Barbosa

    2008-08-01

    Full Text Available As plaquetas chegam rapidamente ao local da ferida e liberam múltiplos fatores de crescimento (FC e citocinas que contribuem para a reparação óssea e aumentam a vascularização local. O Plasma Rico em Plaquetas (PRP concentra as plaquetas e os FC liberados por elas, aceleram a formação óssea e melhora a qualidade do trabeculado. Este trabalho apresenta um protocolo para confecção de PRP e demonstra alguns aspectos da sua utilização na reparação óssea de cães. O protocolo foi desenvolvido a partir de sangue coletado por punção jugular em três cães adultos, pesando em média 20kg. Para avaliação da aplicação clínica e dos aspectos da reparação óssea, foram criadas duas falhas mediais no terço proximal de cada tíbia. Assim, a falha 1 não foi preenchida, constituindo o controle, a falha 2 foi preenchida com 3mg de enxerto ósseo autógeno da crista da tíbia, a falha 3 com gel de plaquetas (PRP e falha a número 4 com a associação PRP e 3mg de enxerto ósseo autógeno. O protocolo laboratorial proposto mostrou-se de fácil execução e de baixo custo e possibilitou a concentração adequada de plaquetas no PRP final, cujo número foi dependente da contagem inicial no sangue total de cada animal. A comparação da radiopacidade na região da falha, em todos os tratamentos, e ao longo do tempo demonstrou que o PRP associado ao enxerto determinou maior precocidade e uniformidade de radiopacidade, quando comparado à falha preenchida pelo PRP e ao enxerto usados isoladamente, e sendo que ambos determinam melhores resultados de preenchimento que a falha mantida sem tratamento.The platelets arrive quickly at the injury site and release several growth factors (GF and citokines that contribute to bone repair and increasing local vascularization. The Platelet-rich Plasma (PRP concentrates the platelets and their growth factors, increasing the rate of bone formation and better quality of trabecular bone. This research presents

  14. ASSESSMENT STUDY ON A SET OF PLATELET-RICH PLASMA PREPARATION%富血小板血浆制备套装的评估研究

    Institute of Scientific and Technical Information of China (English)

    李明; 张长青; 袁霆; 陈圣宝; 吕汝举

    2011-01-01

    目的 通过分析富血小板血浆(platelet-rich plasma,PRP)中血小板、白细胞和生长因子的浓度,计算回收率和富集系数,并做相关性分析,探讨PRP制备套装的实用性和稳定性.方法 取30例符合纳入标准的自愿者自愿捐赠的外周血各40 mL,应用山东威高集团医用高分子制品股份有限公司的PRP制备套装制备PRP各4 mL.全自动血液分析仪计数全血和PRP中血小板和白细胞浓度,并计算血小板或白细胞同收率及富集系数;并分别测定男、女自愿者血小板及白细胞浓度.ELISA法定量分析激活后全血及PRP中PDGF、TGF-β、VEGF的浓度.结果 全血和PRP中血小板浓度分别为(131.40 ±29.44)×109/L和(819.47 ±136.32)×109/L,比较差异有统计学意义(t=-27.020,P=0.000);PRP中血小板回收率为60.85%±8.97%,富集系数为6.40 ±1.06.全血和PRP中自细胞浓度分别为(5.57 ±1.91)×1012/L和(32.20±10.42)× 1012/L,比较差异有统计学意义(t=-13.780,P=0.000);PRP中自细胞回收率为58.30%±19.24%,富集系数为6.10±1.93.PRP中血小板浓度和白细胞浓度分别与全血中血小板浓度(r=0.652,P=0.000)和白细胞浓度(r=0.460,P=0.011)成正相关.男性组和女性组PRP中血小板浓度和白细胞浓度比较差异均无统计学意义(P>0.05).PRP中PDGF、TGF-β、VEGF浓度分别为(698.15 ±64.48)、(681.36±65.90)、(1 071.55 ±106.04)ng/mL,是全血的(5.67 ±1.18)、(6.99 ±0.61)、(5.74 ±0.83)倍.PRP中PDGF浓度(r=0.832,P=0.020)、TGF-β浓度(r=0.835,P=0.019)、VEGF浓度(r=0.824,P=0.023)均与PRP中血小板浓度成正相关.结论 PRP制备套装可以稳定地制备出富含高浓度血小板、白细胞和牛长因子的PRP.%Objective To calculate the recovery rate and enrichment factor and to analyse the correlation by measuring the concentrations of platelets, leukocyte, and growth factors in platelet-rich plasma (PRP) so as to evaluate the feasibility and stability of a set of PRP preparation. Methods

  15. EFFECT OF PLATELET-RICH PLASMA ON TENDON HEALING%富血小板血浆对肌腱愈合影响的实验研究

    Institute of Scientific and Technical Information of China (English)

    耿震; 王宸; 周海洋

    2011-01-01

    Objective Platelet-rich plasma (PRP) secretes many growth factors, including transforming growth factor β1 (TGF-β1), platelet derived growth factor, vascular endothlial growth factor, insulin-like growth factor 1, and so on,which can promote cell proliferation, chemotaxis, and collagen synthesis in wound healing. To investigate the effects of PRP on the tendon healing, and to explore the mechanism of action so as to provide the experimental basis for the tissue engineered tendons. Methods Forty healthy New Zealand white rabbits, weighing 2.5-3.0 kg and male or female, were randomly divided into the experimental group (n=20) and the control group (n=20). PRP was prepared from arterial blood of rabbit's ears through twice centrifugation method of Landesberg. The platelet concentrations of whole blood and PRP were determined. The right achilles tendons of the rabbits were transected to make rupture models. In experimental group, the tendon was sutured after PRP (0.5 mL) was immediately applied at repair site. In control group, the tendon was sutured directly after transection.At 1, 2, 4, and 6 weeks after operation, the tendons of 5 rabbits in each group were harvested for morphological, histological,and immunohistochemical observations; the fibroblast counting, the content of collagen fibers, and the expression of TGF-β1 were detected. Results The concentration of platelet of PRP was 4.03 times of whole blood. All the animals survived till the end of the experiment, and the incision healed well. No death, infection, and other complications occurred. With time, the tendons almost healed in 2 groups, and the fibrous tissue at anastomosis site was more remarkable in control group than in experimental group. The histological observation showed significant differences in fibroblast counting at 1, 2, and 4 weeks after operation between 2 groups (P < 0.05), while no significant difference at 6 weeks (P > 0.05). The contents of collagen fibers in the parenchyma at

  16. Plasma rico en plaquetas: fundamentos biológicos y aplicaciones en cirugía maxilofacial y estética facial Platelet-rich plasma: biology and applications in maxillofacial surgery and facial aesthetics

    Directory of Open Access Journals (Sweden)

    Jordi Rodríguez Flores

    2012-03-01

    Full Text Available Esta revisión presenta el uso del preparado de plasma rico en plaquetas (PRP como una herramienta potenciadora de ciertas características histológicas de los tejidos conformados por células con receptores para los factores de crecimiento plaquetarios. Se ha realizado una revisión sobre el plasma rico en plaquetas de la literatura publicada en PubMed. Se describen los fundamentos biológicos del PRP a nivel celular, la técnica de obtención, y sus usos clínicos en la actualidad.This review shows platelet-rich-plasma (PRP as an enhancer of the histological characteristics of the tissues with platelet-derived growth factor receptors. A review has been made of the literature on the platelet-rich plasma published in PubMed. The article describes the cellular biology of PRP, the technique for obtaining it, and its clinical applications.

  17. 富血小板血浆在口腔医学领域内的应用现状(综述)%Application of platelet rich plasma in Stomatology

    Institute of Scientific and Technical Information of China (English)

    黄伟; 程旭; 刘红红

    2015-01-01

    富血小板血浆因其特殊的作用被广泛运用于医学领域,在口腔医学领域主要被应用在骨组织重建,种植体周围骨缺损修复,牙周组织再生,口腔颌面部软组织创伤修复,组织工程学等方面,该文简要叙述了富血小板血浆在制取以及口腔医学领域的相关研究现状,并讨论了目前临床上存在的一些问题.%Due to its unique function, platelet rich plasma has been used widely in medical field. In terms of stomatology, it is mainly used for bone tissue reconstruction, dental implantation defect repair, Periodontal tissue re-generation, oromaxillo-facial region soft tissue wound healing, tissue engineering and etc. This paper outlines current research on the preparation of platelet rich plasma and related medical research, and discusses the existing problems in clinical practice.

  18. Association of mesenchymal stem cells with platelet rich plasma on the repair of critical calvarial defects in mice Associação de células-tronco mesenquimais com plasma rico em plaquetas na reparação de defeitos críticos em calvária de camundongos

    Directory of Open Access Journals (Sweden)

    Betânia Souza Monteiro

    2012-03-01

    Full Text Available PURPOSE: To evaluate the effects of mesenchymal stem cells (MSC from eight mice C57BL/6 gfp+ bone marrows expanded in cultures associated with platelets rich plasma (PRP deriving from another eight mice, in the repair of critical defects in calvarial bone produced in twenty-four adult isogenic mice C57BL/6. METHODS: The animals were submitted to a cranial defect of 6.0mm in diameter and divided into two equal experimental groups. Control group did not receive treatment and the treated group received a MSC pellet containing 1.0 x 10(7 cells/mL associated with 50.0µL of plasma gel containing 1.0 x 10(9 autologous platelets within the defect. RESULTS: In the treated group was observed process of angiogenesis and bone repair better than control group. CONCLUSION: Mesenchymal stem cells derived from bone marrow of C57BL/6 gfp+ mice associated with PRP gel applied in bone critical defects produced in calvarial contributes positively to the process of bone repair.OBJETIVO: Avaliar os efeitos da associação das células-tronco mesenquimais (MSC oriundas da medula óssea de oito camundongos jovens C57BL/6 gfp+ e expandidas em culturas, com Plasma Rico em Plaquetas (PRP provenientes de outros oito camundongos, na reparação de defeitos críticos confeccionados em calvária de 24 camundongos adultos C57BL/6. MÉTODOS: Os animais foram submetidos a um defeito craniano de 6,0mm de diâmetro e separados em dois grupos experimentais iguais. O grupo controle não recebeu tratamento e no grupo tratado foi administrado, no interior do defeito, pellet de MSC contendo 1,0 x 10(7 células/mL associado com 50,0µL de plasma em gel autólogo contendo 1,0 x 10(9 plaquetas. RESULTADOS: No grupo tratado verificou-se processo de angiogênese e reparação óssea superior ao grupo controle. CONCLUSÃO: A associação das células-tronco mesenquimais (MSC derivadas da medula óssea de camundongos C57BL/6 gfp+ com gel de PRP aplicadas em defeitos ósseos cr

  19. Actualización bibliográfica sobre el uso de preparaciones ricas en plaquetas en la cicatrización de heridas Updated review of the use of platelet rich preparations in wound healing

    Directory of Open Access Journals (Sweden)

    D.A. Mateo de Acosta Andino

    2010-09-01

    Full Text Available Las plaquetas poseen más de 20 factores de crecimiento en sus gránulos. Su secreción promueve la reparación de heridas. La cicatrización es un intrincado proceso en el cual intervienen no solo los factores plaquetarios proangiogénicos sino también factores antiangiogénicos. La preservación por congelación de las plaquetas empleada para su preservación, desestabiliza y activa la membrana plaquetaria alterando sus propiedades. En el campo del tratamiento de heridas recalcitrantes en pacientes debilitados o con enfermedades crónicas, el uso de preparaciones plaquetarias en forma de plasma fresco congelado, las preparaciones plaquetarias sonicadas, las preparaciones congeladas y deshidratadas y el uso de fragmentos de dermis alogénica, confiere efectos beneficiosos, logrando mejores tiempos de cierre en las heridas y menor alteración de la morfología de la cicatriz final. En el presente artículo, describiremos los estudios que han demostrado estos efectos, así como las encrucijadas actuales y los estudios que debemos esperar en un futuro no lejano.Platelets store more than 20 growth factors in their granules. Their secretion promotes wound healing and repair. The intricate wound healing process involves not only the angiogenic platelet factors but also de anti-angiogenic ones. The process of platelet preservation, such as freezing, disrupt the platelet membrane structure and their angiogenic properties causing platelet activation. In the field of wound healing and the management of recalcitrant wounds in debilitated and chronically ill patients, there have been several studies. These studies have focused on the use of the different platelet preparations such as fresh frozen plasma, frozen-dried platelet rich plasma, sonicated platelet rich plasma and the use of alogenic acelular dermis. Platelet preparations posses positive effects on wound healing with little alterations of the final scar tissue. In this article we will describe

  20. Advances in the research of promoting healing of chronic wound with platelet-rich plasma%富血小板血浆促进慢性创面愈合研究进展

    Institute of Scientific and Technical Information of China (English)

    刘宸; 章宏伟

    2014-01-01

    Normal wound healing is a well-orchestrated process of inflammatory response,cell proliferation,and tissue remodeling.However,this orderly and precise process is impaired in chronic wounds.A series of complicated pathogenic factors,including enhanced inflammatory response,poor cell proliferation,restrained angiogenesis,restricted collagen deposition,and infection,contribute to the failure of healing of chronic wound.The application of platelet-rich plasma (PRP) has been explored as a treatment for chronic wounds as it can balance wound microenvironment for promoting wound healing.PRP can modulate the inflammatory mediators,growth factors,and cytokines,etc.to correct abnormal biological events and disorderly molecular environment of cell migration and proliferation,and thus promote wound healing appropriately.Yet,the mechanism of PRP in promoting healing of chronic wound is still not elaborated,and the clinical application of PRP needs to be standardized as soon as possible.

  1. Review of the Application of Platelet-rich Plasma in Osteogenesis%富血小板血浆促进成骨作用的研究进展

    Institute of Scientific and Technical Information of China (English)

    袁道英; 杨佑成

    2007-01-01

    富血小板血浆(platelet-rich plasma,PRP)是自体全血经过梯度离心、分离得到的血小板浓缩物,血小板含量丰富。当血小板激活时,能释放多种生长因子.如血小板源性生长因子(platelet-derived growth factor,PDGF),转化生长因子-β(transforming growth factor-β,TGF-β),血管内皮生长因子(vascular endothelid growth factor,VEGF).

  2. Comparative evaluation of clinical efficacy of β-tri calcium phosphate (Septodont-RTR TM alone and in combination with platelet rich plasma for treatment of intrabony defects in chronic periodontitis

    Directory of Open Access Journals (Sweden)

    Jyostna Pinipe

    2014-01-01

    Full Text Available Aim: To assess the clinical outcome by comparing β-tri calcium phosphate (Septodont RTR TM along with platelet rich plasma (PRP and β-tri calcium phosphate (β-TCP alone in intrabony defects, by clinical evaluation in a 6-month analysis. Methodology: Ten patients participated in the study. Using a split-mouth design, interproximal bony defects were surgically treated with either platelet rich plasma (PRP combined with β-tri calcium phosphate (β-TCP or β-TCP alone. Plaque Index (PI, Gingival Index, Probing Pocket Depth (PPD, Clinical Attachment Level (CAL were recorded 6 months after surgery. Results: At 6 months after therapy, the PRP combined with β-TCP group showed mean PPD reduction of 2.50, CAL mean gain of 2.60 + 1.43. The β-TCP group showed mean PPD reduction of 2.80 mm, mean CAL gain of 2.60 mm. On intra-group comparison, there was greater PPD reduction and CAL gain at 6 months in both the groups. In intergroup comparison of PRP/β-TCP and β-TCP alone, there was no statistical significant difference observed. (P = 0.55, and 0.87 for PPD and CAL gain. Conclusion: Both therapies resulted in significant PPD reduction, CAL gain. The present study shows that treatment of intrabony periodontal defects with combination of PRP and β-TCP does not have additional improvements when compared with β-TCP alone within 6 months follow-up.

  3. RECONSTRUCTION OF THE SELLAR FLOOR FOLLOWING TRANSSPHE-NOIDAL SURGERY USING GELATIN FOAM AND FIBRIN GLUE

    Institute of Scientific and Technical Information of China (English)

    Jian Yin; Chang-bao Su; Zhi-qin Xu; Xue-wei Xia; Fei Song

    2005-01-01

    Objective To introduce a new principle of sellar reconstruction and to evaluate the effectiveness of absorbable gelatin foam and fibrin glue for sellar reconstruction. Methods A total of 176 consecutive patients who underwent surgery for pituitary adenomas, cysts, chordomas, or subdiaphragmatic craniopharyngiomas in the sella turcica between January 2001 and April 2003 at Peking Union Medical College Hospital were enrolled. Different techniques of sellar closure and indications for each specific condition were retrospectively reviewed. Results Seventy-seven (43.7%) patients developed a visible cerebrospinal fluid (CSF) leakage during surgery. Intra operative CSF leakage were repaired simply with gelatin foam and fibrin glue in 62 (35.2%) patients, and with autologous fat graft and sellar floor reconstruction in 15 (8.5%) patients. Postoperative CSF rhinorrhea occurred only in 1 case. There were no visual deterioration, allergic rhinitis, meningitis, pneumocranium, granulomas, or other complications associated with the reconstruction procedure. Conclusion The procedure of using gelatin foam and fibrin glue and principle of cranial base reconstruction is safe and effective in preventing postoperative complications following transsphenoidal surgery.

  4. Plasma rico em plaquetas combinado a hidroxiapatita na formação do calo ósseo em fraturas induzidas experimentalmente no rádio de cães Platelet-rich plasma combined with hydroxyapatite on bone callus formation in experimental radii fractures in dogs

    Directory of Open Access Journals (Sweden)

    Silmara Bonomi da Silva

    2007-08-01

    Full Text Available Avaliou-se, no presente estudo, os efeitos do plasma rico em plaquetas (PRP autógeno combinado a hidroxiapatita não-absorvível na formação do calo ósseo em fraturas diafisárias induzidas experimentalmente em rádio de cães e fixadas com placas e parafusos ósseos. Foram utilizados oito cães adultos sem raça definida, machos ou fêmeas, com pesos compreendidos entre 5 e 15kg. Induziu-se na diáfise de ambos os rádios defeito ósseo correspondente a 25% do diâmetro do mesmo. No rádio direito (grupo HA/PRP, implantou-se plasma rico em plaquetas combinado a hidroxiapatita. No rádio esquerdo (grupo HA, implantou-se somente a hidroxiapatita. Para a obtenção do PRP, empregou-se protocolo simplificado utilizando centrífuga laboratorial comum. Comparou-se entre os grupos a evolução clínica dos animais, o tempo de formação e a densidade do calo ósseo por exames radiográficos simples e pela técnica de densitometria óptica aos 15, 30, 45 e 60 dias de pós-operatório. Tanto na avaliação radiográfica como densitométrica não se observou diferença estatisticamente significante na evolução do calo ósseo entre os grupos (P>0,05. No presente estudo, o PRP autógeno combinado à hidroxiapatita não-absorvível não acelerou o processo de formação do calo ósseo, comparado à hidroxiapatita somente.The present study was aimed at evaluating the effects of autologous platelet-rich plasma (PRP in combination with unabsorbable hydroxyapatite on bone callus formation in an experimental canine diaphyseal radius fracture stabilized with bone plate. Eight mature, male and female mongrel dogs were selected weighed 5 to 15kg. Bone defects were created, bilateral, in both radii diaphysis, corresponding to 25% of bone diameter. On the right radius (group HA/PRP, the defect was filled with PRP and hydroxyapatite. On the left radius (group HA, was placed hydroxyapatite alone. PRP was obtain by a simplified technique using a laboratory

  5. 富血小板血浆凝胶对家兔骨髓间充质干细胞增殖与成骨活性的影响%Effects of platelet-rich plasma gel on the proliferation and osteogenic activity of rabbit bone marrow mesenchymal stem cells

    Institute of Scientific and Technical Information of China (English)

    张林朴; 代晓华; 王冠华; 李燕妮; 连小丽; 姚晖

    2012-01-01

    BACKGROUND: Platelet-rich plasma (PRP) contains several kinds of growth factors related to bone repair, and can form fibrin meshwork scaffold after agglomerated by activation, which is beneficial to cells adhesion and promotes boneregeneration.OBJECTIVE: To evaluate the proliferation and osteogenic activity of rabbit bone marrow mesenchymal stem cells(rBMSCs) in platelet-rich plasma gel in vitro.METHODS: rBMSCs were isolated from bone marrow of limb long bone of 5-day neonatal rabbits by adhesion culture.PRP was obtained from adult rabbit peripheral venous blood. Experiment samples were divided into three groups: PRPplus rBMSCs (PRP/rBMSCs) group, PRP plus α-MEM medium (PRP/α-MEM) group and rBMSCs group.RESULTS AND CONCLUSION: The difference of lactate dehydrogenase activity among three groups was significant(P < 0.05), and the cell proliferation in PRP/rBMSCs group was obviously better than that in the other two groups. ThePRP/rBMSCs gel was complete, uniform and semitransparent. It had many cells which were anomalistic polygon withlong synapse distributing in gel. Tetracycline labeled flavo-green fluorescence brightened dots and nodes werediscovered in PRP/rBMSCs gel after 2 and 3 weeks of culture using fluorescence microscope. Calcify nodes were formedin the PRP/rBMSCs gel. The number and size of fluorescence brightened dots and nodes founded in the third week afterculture were a little more and bigger than those found in the second week. There were no fluorescence brightened dotsand nodes in PRP/α-MEM gel. It indicates that rBMSCs combined with PRP gel can promote the proliferation andosteogenic activity of rBMSCs.%背景:富血小板血浆含有多种骨组织修复有关的生长刺激因子,并且在凝聚后可以形成纤维蛋白网络支架利于细胞的黏附而促进骨组织再生.目的:体外评价家兔骨髓间充质干细胞在富血小板血浆凝胶中的增殖与成骨活性.方法:分离培养5 d龄新生大耳白兔骨髓间充质干细

  6. A three-dimensional cell-loading system using autologous plasma loaded into a porous {beta}-tricalcium-phosphate block promotes bone formation at extraskeletal sites in rats

    Energy Technology Data Exchange (ETDEWEB)

    Tajima, Nobutaka [Oral and Maxillofacial Surgery, Graduate school, Tokyo Medical and Dental University (Japan); Orthopaedic and Spinal Surgery, Graduate school, Tokyo Medical and Dental University (Japan); Sotome, Shinichi [Orthopaedic and Spinal Surgery, Graduate school, Tokyo Medical and Dental University (Japan); Marukawa, Eriko [Oral and Maxillofacial Surgery, Graduate school, Tokyo Medical and Dental University (Japan); Orthopaedic and Spinal Surgery, Graduate school, Tokyo Medical and Dental University (Japan); Omura, Ken [Oral and Maxillofacial Surgery, Graduate school, Tokyo Medical and Dental University (Japan); Center of Excellence Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical and Dental University (Japan); Shinomiya, Kenichi [Orthopaedic and Spinal Surgery, Graduate school, Tokyo Medical and Dental University (Japan) and Center of Excellence Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical and Dental University (Japan) and Advanced Bone and Joint Science (Japan)]. E-mail: shinomiya.orth@tmd.ac.jp

    2007-05-16

    The effects of platelet-rich plasma (PRP) and platelet-poor plasma (PPP) on bone marrow stromal cells (MSCs) with respect to proliferation, osteogenic differentiation, and bone formation capability were investigated. MSCs derived from rats were cultured in medium containing mixtures of PRP and PPP. Fibrinogen was eliminated prior to the experiment. The DNA content and alkaline phosphatase (ALP) activity were measured. PRP stimulated cell proliferation and inhibited osteoblastic differentiation. To examine the effects of fibrin in plasma, MSCs were cultured in PRP or PPP fibrin gels formed both on a cell culture insert installed in a culture well and on the bottom surface of the same culture well. The ALP activities of the MSCs in both of the gels were higher than those on the surface of the culture wells. The MSCs cultured on the PPP gel showed the highest ALP activity. The effects of PRP and PPP used as scaffolds for bone formation were also investigated. MSCs were suspended in PRP or PPP, introduced into porous {beta}-tricalcium phosphate blocks, and then implanted into subcutaneous sites. Subsequently, bone formation was quantified. Further in vivo studies found that implants prepared using PPP had a greater osteoinductive capability than implants prepared with PRP.

  7. Promotive effect of platelet-rich plasma on proliferation of corneal endothelial cells in cats%富血小板血浆促猫角膜内皮细胞增殖的研究

    Institute of Scientific and Technical Information of China (English)

    王新法; 徐锦堂

    2011-01-01

    目的 观察富血小板血浆对体外培养猫角膜内皮细胞增殖的影响.方法 采用揭膜法与消化法相结合获取猫角膜原代内皮细胞,培养基采用DMEM培养基(含体积分数10%胎牛血清).采用二次离心法提取富血小板血浆.在完全培养基中加入体积分数分别为5%、10%、20%的富血小板血浆培养角膜内皮细胞,以未加入富血小板血浆的完全培养基为对照.CCK-8试剂盒检测细胞增殖情况,扫描电镜观察细胞形态变化.结果 富血小板血浆体外培养角膜内皮细胞时间越长,其促增殖作用越明显,与对照组比较差异均有显著统计学意义意义(均为P <0.01),且随着富血小板血浆含量的增加,角膜内皮细胞增殖越明显,呈剂量依赖性.扫描电镜检测表明,与对照组相比,PRP作用组角膜内皮细胞表面可见丰富的微绒毛,且随着富血小板血浆含量的增加,微绒毛越丰富.结论 富血小板血浆能明显促进体外培养猫角膜内皮细胞增殖.%Objective To observe effect of platelet-rich plasma (PRP) on proliferation of corneal endothelial cells (CEC) in cats in vitro. Methods The cat CEC were obtained by separating and treating with enzyme digestion,then cultured in DMEM supplemented with 10% volume fractions fetal bovine serum (FBS). PRP was extracted by the two-step centrifugation method. The CEC of the second generation were cultured in the complete media containing different concentrations of PRP (5% , 10% and 20% volume fractions),while those cultured in the media without PRP were served as controls. Proliferation of CEC was detected by CCK-8 kit method. The morphological changes of CEC were examined by scanning electron microscope (SEM). Results Effect of proliferation of the CEC cultured with PRP was obvious with time prolonged, and had statistical difference compared with that of control group (P < 0. 01). The proliferation of CEC was more obvious with the increase of platelet-rich

  8. 富血小板血浆对光老化皮肤中胶原蛋白的影响%The effect of platelet rich plasma on collagen in the photoaging skin

    Institute of Scientific and Technical Information of China (English)

    童怡兰; 王松山; 李爱林; 谢亮; 熊菡萏; 李倩倩; 郑梁

    2014-01-01

    Objective Through the establishment of photoaging skin model,research the change in the photoaging skin model,and the Anti-aging effect of platelet rich plasma (PRP) in the photoaging skin.Methods Twelve healthy Big ear rabbits were randomly divided into two groups:experimental group and control group.After molding,take the back of the neck skin do conventional HE staining,observe cells from the light-microscopy; Then,the control group use the normal saline for hypodermic on the back of the neck skin,while the experimental group use platelet rich plasma (PRP).Take the back of the neck skin to do conventional HE staining before and after injection,observe cells from the light-microscopy; Immunohistochemistry analysis the collagen.Results Under light-microscopy,control group did not change significantly.But in experimental group,collagen fibers packed tightly and the total area of elastic fibers increase d.Mature collagen fibers increased significantly with the nascent collagen fibers reduced; After injection of saline and platelet rich plasma (PRP),The expressions of collagen Ⅰ and collagen Ⅲ did not change obviously (P > 0.05),but in the experimental group,the expressions obviously changed (P <0.05).Conclusion As the indicator of skin aging,the expressions of collagen Ⅰ and collagen Ⅲ changed significant after PRP injection.%目的 通过建立光老化皮肤模型,观察富血小板血浆(PRP)对光老化皮肤中的抗衰老作用.方法 SPF级大耳白兔12只,随机分为实验组和对照组.采用UVA灯照射颈部及背部裸露皮肤24 h的方法制备光老化模型,实验组用PRP注射到真皮浅层,对照组则为生理盐水注射.分别于注射前后取颈背部皮肤行常规苏木素-伊红(HE)染色,光镜下观察细胞;免疫组织化学法分析Ⅰ型胶原及Ⅲ型胶原.结果 对照组HE染色未见明显改变,实验组较前胶原纤维排列紧密,弹力纤维总面积增加,成熟胶原纤维明显增多,新生的胶原纤

  9. Thrombin interaction with fibrin polymerization sites.

    Science.gov (United States)

    Hsieh, K

    1997-05-15

    Thrombin is central to hemostasis, and postclotting fibrinolysis and wound healing. During clotting, thrombin transforms plasma fibrinogen into polymerizing fibrin, which selectively adsorbs the enzyme into the clot. This protects thrombin from heparin-antithrombin inactivation, thus preserving the enzyme for postclotting events. To determine how the fibrin N-terminal polymerization sites of A alpha 17-23 (GPRVVER) and B beta 15-25 (GHRPLDKKREE) and their analogs may interact with thrombin, amidolysis vs. plasma- and fibrinogen-clotting assays were used to differentiate blockade of catalytic site vs. other thrombin domains. Amidolysis studies suggest GPRVVER inhibition of thrombin catalytic site through hydrophobic interaction, and GPRVVER inhibited clotting. Neither GPRP nor VVER nor the B beta 15-25 homologs inhibited amidolysis. Contrary to heparin, acyl-DKKREE promoted plasma-clotting, but inhibited fibrinogen-clotting. In addition, acyl-DKKREE reversed the anticoagulant effect of heparin (0.1 U/ml) in plasma. The results suggest fibrin B beta 15-25 interaction with thrombin, possibly by blocking the heparin-binding site. Together with the reported fibrin A alpha 27-50 binding to thrombin, polymerizing fibrin appears to initially bind to thrombin catalytic site and exosite-1 through A alpha 17-50, and to another thrombin site through B beta 15-25. As these fibrin sites are also involved in polymerization, competition of the polymerization process with thrombin-binding could subsequently dislodge thrombin from fibrin alpha-chain. This may re-expose the catalytic site and exosite-1, thus explaining the thrombogenicity of clot-bound thrombin. The implications of these findings in polymerization mechanism and anticoagulant design are discussed.

  10. Platelet-rich plasma scaffolds induce dental pulp-like tissue regeneration in vivo%富血小板血浆支架诱导牙髓再生的体内研究

    Institute of Scientific and Technical Information of China (English)

    韦维; 黄杨; 李康婧; 陈文霞

    2014-01-01

    Objective To evaluate the in vivo dental pulp regeneration capacity of various concentration of the platelet-rich plasma (PRP). Methods The tooth roots of minipigs were prepared by chemical methods. PRP was extracted by two-step centrifugation. The roots were divided into four groups according to the kinds of scaffold injected into the root canals (5 roots each group): (1)control group, whole blood; (2)100% PRP; (3)50% PRP; (4)blank group: root fragments with empty canal space. The roots were implanted subcutaneously in nude mice. The animals were sacrificed after 5 weeks. The samples were taken for histological examination . Results The newly formed pulp-like tissues were observed in the roots which filled 50% PRP scaffolds in canals . The group of 100% PRP scaffolds were full of inflammatory cells. Conclusions It is feasible for the platelet-rich plasma with proper concentration as scaffolds to induce dental pulp regeneration in vivo.%目的:探讨不同浓度的富血小板血浆(PRP)支架在体内诱导牙髓组织再生的能力。方法将小型猪乳牙牙根段进行化学预备,采用二次离心法制备PRP,根据注入根管中的成分不同将研究分为4组:(1)阴性对照组,即全血组;(2)100% PRP组;(3)50% PRP组;(4)空白组,即空的牙根段;每组5个样本,分别植入裸鼠背部皮下,于术后5周处死动物,取出样本进行组织学观察。结果植入5周后,100% PRP组根管内充满了炎性细胞,50% PRP组根管内有少量牙髓样的组织生成。结论合适浓度的PRP作为生物支架在体内再生牙髓样的组织是可行的。

  11. 富血小板血浆用于犬牙直接盖髓的实验研究%Platelet rich plasma as direct pulp capping agent:An experimental study in dogs

    Institute of Scientific and Technical Information of China (English)

    石珍; 屈铁军; 程薇; 张亚庆; 范晓敏

    2012-01-01

    目的:观察富血小板血浆(platelet- rich plasrna,PRP)直接盖髓后修复性牙本质的形成.方法:成年雄性健康杂种犬2只,分别取静脉血制备PRP后,选取每只犬的所有切牙,尖牙和前磨牙共48个牙作为对象,于颈部制作穿髓模型后,按拆分口设计原则随机分为6组(MTA、Dycal、MTA+ PRP、Dycal+ PRP、PRP、空白),分别用相应盖髓剂进行直接盖髓治疗,3个月后应用牙科CT观察各组穿髓处硬组织和牙本质桥形成情况.结果:实验3个月后,MTA,MTA+ PRP,Dycal+ PRP组修复性牙本质形成的效果均优于单纯氢氧化钙组(Dycal).PRP组、空白组无完整的牙本质桥形成.结论:PRP+Dycal能促进犬富血小板血浆牙髓组织修复,是一种很有希望的复合型生物盖髓剂.%AIM: To observe the formation of reparative dentin after direct pulp capping with platelet rich plasma (PRP). METHODS: Venous blood was taken from two adult male mongrel dogs to prepare PRP. A total of 48 teeth, including all incisors, canines and premolars, were drilled to expose pulps at the neck. A split-mouth design and intra animal side randomization were applied to divide the teeth int 6 groups. The pulp was directly capped with capping agents (MTA, Dycal, MTA+PRP, Dycal + PRP, PRP and blank) respectively. Three months after capping, the hard tissue and dentin bridge formation were evaluated at the exposed pulp by Dental CT. RESULTS: Reparative dentin formation in MTA, MTA + PRP and Dycal + PRP groups were significantly enhanced as compared with calcium hydroxide only group {Dycal). No complete dentin bridge was formed in PRP group and blank group. CONCLUSION : PRP plus Dycal can promote the regeneration of dog pulp tissues, and shows promising results as a biological pulp capping agent.

  12. Platelet-rich plasma in bone repair of irradiated tibiae of Wistar rats Plasma rico em plaquetas na reparação óssea em tíbias irradiadas de ratos Wistar

    Directory of Open Access Journals (Sweden)

    Emne Hammoud Gumieiro

    2010-06-01

    Full Text Available PURPOSE: To evaluate the influence of PRP addition on bone repair of circular defects created in irradiated tibiae of rats by histometric analysis. METHODS: Sixty male Wistar rats had the right tibiae irradiated with 30 Gy. After 30 days monocortical defects were created and platelet-rich plasma was applied in 30 rats. In the control group defects were created but not filled. The animals were desanguinated after 4, 7, 14, 21, 56 and 84 days and the tibiae removed for histological processing. RESULTS: There was a tendency in the PRP group to increased bone neoformation from 14-days to 84-days; in the control group increased bone neoformation was not seen after 21 days or later. CONCLUSION: The addition of platelet-rich plasma had a beneficial effect in the initial cellular regeneration period and enhanced bone formation in later periods when compared to control.OBJETIVO: Avaliar histometricamente a influência do PRP na reparação óssea de defeitos circulares criados em tíbia irradiada de ratos Wistar. MÉTODOS: Sessenta ratos machos tiveram a tíbia direita irradiada com 30Gy. Após 30 dias, defeitos ósseos monocorticais foram criados e PRP foi adicionado em 30 ratos. No grupo controle os defeitos foram criados, mas não preenchidos. Os animais foram sacrificados em 4, 7, 14, 21, 56 e 84 dias e a tíbia removida para processamento histológico. RESULTADOS: Houve uma tendência do grupo PRP mostrar uma neoformação óssea significativamente maior nos períodos de 14 a 84 dias; no grupo controle o aumento da reparação óssea não se manteve após 21 dias. CONCLUSÃO: A adição de PRP mostrou-se benéfica no período inicial de reparação celular e em períodos seqüentes foi estímulo à quantidade neoformação óssea, quando comparado ao controle.

  13. Evolution of fibrin glue applicators.

    Science.gov (United States)

    Marx, Gerard

    2003-10-01

    Fibrin glue (FG) is used worldwide as a potent surgical tool, which establishes hemostasis in wounds and also bonds tissue. The standard FG applicator is based on a dual-syringe system. This review, based mainly on the patent literature, describes the development of the quasi-standard dual syringe system as well as the rise of other FG applicator designs based on mechanical force (ratchet systems), Bernoulli gas flow, positive gas pressure, or electro-servo devices. The packaging of commercial FG components is reviewed within the context of "loading" the FG applicators and the need to minimize the number of needles required to access the packaged (vials) components. Parameters such as internal clogging, homogeneity of spray, the requirement for gas or vacuum house lines, the number of parts that must be handled, and the time required to assemble the applicator, load it, and have it ready for use are also discussed. A rating system is proposed that permits one to use such parameters to rank the various applicator designs, relative to the dual-syringe system. Hopefully, this review will stimulate the design of better FG applicators and packaging required for elective surgery, emergency treatments, and tissue engineering in the 21st century.

  14. 21 CFR 864.7300 - Fibrin monomer paracoagulation test.

    Science.gov (United States)

    2010-04-01

    ... to detect fibrin monomer in the diagnosis of disseminated intravascular coagulation (nonlocalized clotting within a blood vessel) or in the differential diagnosis between disseminated intravascular coagulation and primary fibrinolysis (dissolution of the fibrin in a blood clot). (b) Classification. Class...

  15. Fibrin sealant for mesh fixation in laparoscopic umbilical hernia repair

    DEFF Research Database (Denmark)

    Eriksen, J R; Bisgaard, T; Assaadzadeh, S;

    2013-01-01

    Fibrin sealant for mesh fixation has significant positive effects on early outcome after laparoscopic ventral hernia repair (LVHR) compared with titanium tacks. Whether fibrin sealant fixation also results in better long-term outcome is unknown.......Fibrin sealant for mesh fixation has significant positive effects on early outcome after laparoscopic ventral hernia repair (LVHR) compared with titanium tacks. Whether fibrin sealant fixation also results in better long-term outcome is unknown....

  16. Laryngospasm after autologous blood transfusion.

    Science.gov (United States)

    Hong, Jung; Grecu, Loreta

    2006-07-01

    Although perioperative autologous blood transfusions are associated with few side effects, transfusion reactions can occur and can be life-threatening. We report the occurrence of postoperative laryngospasm in a patient who underwent spinal anesthesia for hip surgery. The laryngospasm could not be attributed to any cause other than the autologous blood transfusion and recurred when the transfusion was restarted. Laryngospasm was successfully treated both times with positive pressure ventilation. Autologous transfusions can trigger febrile nonhemolytic transfusion reactions, which may result in airway compromise.

  17. Autologous bone marrow stem cell intralesional transplantation repairing bisphosphonate related osteonecrosis of the jaw

    Directory of Open Access Journals (Sweden)

    Cella Luigi

    2011-08-01

    Full Text Available Abstract Purpose Bisphosphonate - related osteonecrosis of the JAW (BRONJ is a well known side effect of bisphosphonate therapies in oncologic and non oncologic patients. Since to date no definitive consensus has been reached on the treatment of BRONJ, novel strategies for the prevention, risk reduction and treatment need to be developed. We report a 75 year old woman with stage 3 BRONJ secondary to alendronate and pamidronate treatment of osteoporosis. The patient was unresponsive to recommended treatment of the disease, and her BRONJ was worsening. Since bone marrow stem cells are know as being multipotent and exhibit the potential for differentiation into different cells/tissue lineages, including cartilage, bone and other tissue, we performed autologous bone marrow stem cell transplantation into the BRONJ lesion of the patient. Methods Under local anesthesia a volume of 75 ml of bone marrow were harvested from the posterior superior iliac crest by aspiration into heparinized siringes. The cell suspension was concentrated, using Ficoll - Hypaque® centrifugation procedures, in a final volume of 6 ml. Before the injection of stem cells into the osteonecrosis, the patient underwent surgical toilet, local anesthesia was done and spongostan was applied as a carrier of stem cells suspension in the bone cavity, then 4 ml of stem cells suspension and 1 ml of patient's activated platelet-rich plasma were injected in the lesion of BRONJ. Results A week later the residual spongostan was removed and two weeks later resolution of symptoms was obtained. Then the lesion improved with progressive superficialization of the mucosal layer and CT scan, performed 15 months later, shows improvement also of bone via concentric ossification: so complete healing of BRONJ (stage 0 was obtained in our patient, and 30 months later the patient is well and without signs of BRONJ. Conclusion To our knowledge this is the first case of BRONJ successfully treated with

  18. 自体富含血小板血浆痛点注射治疗慢性跟腱炎15例分析%Platelet-rich plasma trigger point injection treatment for chronic achilles insertion tendonitis

    Institute of Scientific and Technical Information of China (English)

    邹国友; 贾伟涛; 郑闽前; 徐小卒; 曹正春; 殷俊; 吴亚

    2013-01-01

    Retrospective analysis was performed for the clinical data of 15 chronic insertion achilles tendinitis patients undergoing platelet-rich plasma (PRP) trigger point injection.The scores of Validated Victorian Institute of Sports Assessment-Achilles (VAS-A) and foot function index (FFI) improved greatly versus pre-treatment (all P < 0.05).Tendon insertion structure inflammation decreased significantly on magnetic resonance imaging.At the last follow-up,all patients recovered normal gait and daily activity.The trigger point injection of PRP is efficacious for chronic insertion achilles tendinopathy.%回顾分析15例慢性跟腱炎采用自体富含血小板血浆(PRP)痛点注射治疗患者的临床资料.每例患者3~5个痛点共注射2 ml PRP[血小板浓度为(1643±180)×109/L],治疗后维多利亚医学院跟腱评分和足功能指数(FFI)与治疗前相比差异有统计学意义(均P<0.05);MRI显示跟腱炎周围的软组织炎症明显改善;患者恢复了正常步态和日常活动能力.

  19. Regenerative Endodontic Therapy in a Single Visit Using Platelet-rich Plasma and Biodentine in Necrotic and Asymptomatic Immature Molar Teeth: A Report of 3 Cases.

    Science.gov (United States)

    Topçuoğlu, Gamze; Topçuoğlu, Hüseyin Sinan

    2016-09-01

    This report describes 3 successful single-visit regenerative endodontic therapy cases using platelet-rich plasma (PRP) and Biodentine (Septodont, Saint Maurdes Fossés, France) for 3 immature mandibular molar teeth, all with necrotic pulp, in 2 children aged 8 years and 1 aged 9 years. Three teeth were separately diagnosed as having a necrotic pulp. After preparation of the access cavity under rubber dam isolation, the necrotic pulp was removed, and each canal was irrigated with 2.5% sodium hypochlorite, sterile saline, and 17% EDTA solutions. Freshly prepared PRP was injected into each canal up to the cementoenamel junction, and Biodentine was placed directly over the PRP clot. Each access cavity was then restored with composite resin. Follow-up clinical examinations revealed negative responses to cold and electric pulp tests. None of the treated teeth were sensitive to percussion or palpation. Radiographic examination showed continued thickening of root canal walls and apical closure of the root apex of each tooth. Single-visit regenerative endodontic therapy can be considered in necrotic and asymptomatic immature permanent teeth. PRP and Biodentine may serve as scaffold and barrier materials in regenerative endodontic procedures. PMID:27427186

  20. Platelet-Rich Plasma-Loaded Poly(d,l-lactide)-Poly(ethylene glycol)-Poly(d,l-lactide) Hydrogel Dressing Promotes Full-Thickness Skin Wound Healing in a Rodent Model

    Science.gov (United States)

    Qiu, Manle; Chen, Daoyun; Shen, Chaoyong; Shen, Ji; Zhao, Huakun; He, Yaohua

    2016-01-01

    Traditional therapeutic methods for skin wounds have many disadvantages, and new wound dressings that can facilitate the healing process are thus urgently needed. Platelet-rich plasma (PRP) contains multiple growth factors (GFs) and shows a significant capacity to heal soft tissue wounds. However, these GFs have a short half-life and deactivate rapidly; we therefore need a sustained delivery system to overcome this shortcoming. In this study, poly(d,l-lactide)-poly(ethylene glycol)-poly(d,l-lactide) (PDLLA-PEG-PDLLA: PLEL) hydrogel was successfully created as delivery vehicle for PRP GFs and was evaluated systematically. PLEL hydrogel was injectable at room temperature and exhibited a smart thermosensitive in situ gel-formation behavior at body temperature. In vitro cell culture showed PRP-loaded PLEL hydrogel (PRP/PLEL) had little cytotoxicity, and promoted EaHy926 proliferation, migration and tube formation; the factor release assay additionally indicated that PLEL realized the controlled release of PRP GFs for as long as 14 days. When employed to treat rodents’ full-thickness skin defects, PRP/PLEL showed a significantly better ability to raise the number of both newly formed and mature blood vessels compared to the control, PLEL and PRP groups. Furthermore, the PRP/PLEL-treated group displayed faster wound closure, better reepithelialization and collagen formation. Taken together, PRP/PLEL provides a promising strategy for promoting angiogenesis and skin wound healing, which extends the potential of this dressing for clinical application. PMID:27347938

  1. Regenerative Endodontic Therapy in a Single Visit Using Platelet-rich Plasma and Biodentine in Necrotic and Asymptomatic Immature Molar Teeth: A Report of 3 Cases.

    Science.gov (United States)

    Topçuoğlu, Gamze; Topçuoğlu, Hüseyin Sinan

    2016-09-01

    This report describes 3 successful single-visit regenerative endodontic therapy cases using platelet-rich plasma (PRP) and Biodentine (Septodont, Saint Maurdes Fossés, France) for 3 immature mandibular molar teeth, all with necrotic pulp, in 2 children aged 8 years and 1 aged 9 years. Three teeth were separately diagnosed as having a necrotic pulp. After preparation of the access cavity under rubber dam isolation, the necrotic pulp was removed, and each canal was irrigated with 2.5% sodium hypochlorite, sterile saline, and 17% EDTA solutions. Freshly prepared PRP was injected into each canal up to the cementoenamel junction, and Biodentine was placed directly over the PRP clot. Each access cavity was then restored with composite resin. Follow-up clinical examinations revealed negative responses to cold and electric pulp tests. None of the treated teeth were sensitive to percussion or palpation. Radiographic examination showed continued thickening of root canal walls and apical closure of the root apex of each tooth. Single-visit regenerative endodontic therapy can be considered in necrotic and asymptomatic immature permanent teeth. PRP and Biodentine may serve as scaffold and barrier materials in regenerative endodontic procedures.

  2. Autologous blood storage in obstetrics.

    Science.gov (United States)

    Herbert, W N; Owen, H G; Collins, M L

    1988-08-01

    Autologous transfusion, storage of one's own blood for subsequent infusion if needed, is safe and effective in a variety of scheduled operative procedures. Obstetric involvement in such programs is very limited, however. Thirty pregnant women with placenta previa or other potential complications underwent 55 phlebotomies in an autologous transfusion program. Phlebotomies were performed at an average gestational age of 32.4 weeks (range 13-40). Changes in mean diastolic blood pressure and pulse were minimal. Electronic fetal monitoring tracings were normal during the 34 procedures in which it was used. The frequency of mild donor reactions (4%) was consistent with that in nonpregnant donors. After entry into this program, 15 patients received a total of 29 U of packed red blood cells (23 autologous; six homologous). Homologous transfusion was avoided in 86.7% of patients receiving blood. Selected pregnant women can participate safely in autologous blood collection programs, minimizing the need, and therefore the risks, of homologous transfusion. PMID:3292974

  3. Encapsulation of cardiomyocytes in a fibrin hydrogel for cardiac tissue engineering.

    Science.gov (United States)

    Yuan Ye, Kathy; Sullivan, Kelly Elizabeth; Black, Lauren Deems

    2011-01-01

    Culturing cells in a three dimensional hydrogel environment is an important technique for developing constructs for tissue engineering as well as studying cellular responses under various culture conditions in vitro. The three dimensional environment more closely mimics what the cells observe in vivo due to the application of mechanical and chemical stimuli in all dimensions (1). Three-dimensional hydrogels can either be made from synthetic polymers such as PEG-DA (2) and PLGA (3) or a number of naturally occurring proteins such as collagen (4), hyaluronic acid (5) or fibrin (6,7). Hydrogels created from fibrin, a naturally occurring blood clotting protein, can polymerize to form a mesh that is part of the body's natural wound healing processes (8). Fibrin is cell-degradable and potentially autologous (9), making it an ideal temporary scaffold for tissue engineering. Here we describe in detail the isolation of neonatal cardiomyocytes from three day old rat pups and the preparation of the cells for encapsulation in fibrin hydrogel constructs for tissue engineering. Neonatal myocytes are a common cell source used for in vitro studies in cardiac tissue formation and engineering (4). Fibrin gel is created by mixing fibrinogen with the enzyme thrombin. Thrombin cleaves fibrinopeptides FpA and FpB from fibrinogen, revealing binding sites that interact with other monomers (10). These interactions cause the monomers to self-assemble into fibers that form the hydrogel mesh. Because the timing of this enzymatic reaction can be adjusted by altering the ratio of thrombin to fibrinogen, or the ratio of calcium to thrombin, one can injection mold constructs with a number of different geometries (11,12). Further we can generate alignment of the resulting tissue by how we constrain the gel during culture (13). After culturing the engineered cardiac tissue constructs for two weeks under static conditions, the cardiac cells have begun to remodel the construct and can generate a

  4. UROKINASE MUTANT WITH BETTER FIBRIN SPECIFICITY

    Institute of Scientific and Technical Information of China (English)

    MaZhong; YuRuirong; HuaZichun; ZhuDexu

    1997-01-01

    Urokinsae (UK),one of the principal activators of plasminogen, has been widely used in the clinical therapy of thrombosis. However ,it lacks fibrin specificity ,and can lead to extensive systemic activation of plasminogen when injected into veins in large doses. On the other hand,

  5. Application of fibrin glue on endoscopic liposuction

    Institute of Scientific and Technical Information of China (English)

    CHEN Zhong-cun; YANG Jia-feng; CHEN Qian-qian

    2001-01-01

    @@ OBJECTIVE To deserve the effects of FG (Fibrin Glue)on endoscopic liposuction. Methods:From Oct. 1998 to Aug. 2000 forty cases were undergoing for the liposuction. Atthe aids of tumescent teachnique, endoscopy and FG were used, of which 20 casesoperation on their abdominal walls, 8 cases upper abdomen walls and 12 cases ownerabdominal.

  6. Platelets and fibrin strands during clot retraction.

    Science.gov (United States)

    Morgenstern, E; Korell, U; Richter, J

    1984-03-15

    The ultrastructure of platelet fibrin contacts (PFC) and the course of the strands was investigated in serial sections of retracted clots with the help of specimen tilting. We found after retraction in a test tube as well as under isometric conditions in the resonance thrombograph, after HARTERT, an uniform type of PFC. The side to side contact between platelet surface and fibrin strands displayed a 15 nm wide space which was bridged of 10 - 30 nm by filamentary structure. In each case the direction of the fibrin strands changed on contact with the platelet surface (bend). These bends recurred if the adhering strands ran over a longer distance on the platelet surface. The bends can be explained by non-directional movement of the platelets or of their pseudopodia. Microfilaments (actomyosin) which run straight in pseudopodia and often also twisted in the platelet body support this assumption. The described mechanism - contact of the thrombin activated platelets with fibrin strands and simultaneous nondirectional movement of the platelets which bind further sections of the adhering strands to their surface - would provide a more satisfactory explanation for the retraction of the clot to 1/10 of its original volume. PMID:6539004

  7. 离心力、时间对富血小板血浆中生长因子浓度的影响%The Influence of Centrifugal Force and Time on Growth Factor Concentration in Platelet Rich Plasma

    Institute of Scientific and Technical Information of China (English)

    林颢; 孙杰聪; 李广盛; 郑锦畅; 魏波

    2014-01-01

    Objective To detect the concentration of TGF-β in platelet rich plasma (PRP) that was prepared under dif erent centrifugal force and time, and make a related analysis. Aiming to investigate the most appropriate centrifugal force and time for preparing PRP. Methods Collect 200ml peripheral blood from volunteers, anticoagulated it with EDTA at stored at 4℃ for later use. Ful-automatic blood analyzer was used for the platelet counting, isolated the PRP by quadratic centrifugation (with dif erent centrifugal force and time),then counted the platelet again with full-automatic blood analyzer. The concentration of TGF-β in PRP was detected by ELISA detection. Results The concentration of platelet in peripheral blood was 146.36í109/L, and in PRP was (757.27±55.07)í109L.The concentration of TGF-β in PRP was (737.68±62.7)ng/ml.We found that the concentration of TGF-β in PRP that isolated under 900g,10min combined with 700g,10min was the highest (881.5í10)9/L,824.2 ng/ml).Conclusion The quality of PRP the preparation is closely related to the centrifugal force and time. The appropriate centrifugation and time is more likely to a get high concentration of PRP that rich in growth factors.%目的探索应用不同的离心速度来制备的富血小板血浆(platelet rich plasma,PRP)中TGF生长因子的含量,并做相关分析,尝试阐明制备PRP最合适的离心次数及速度。方法采集自愿者外周血200ml,应用EDTA抗凝,4º保存以备用。应用全自动血液分析仪进行血小板计数,采用二次离心法(不同的离心速度、时间)分离出PRP,再次用全自动血液分析仪进行血小板计数,然后用ELISA法检测所得的PRP中的TGF-β的浓度。结果外周血中的血小板浓度为146.36×109L,PRP的血小板浓度为(757.27±55.07)×109L。 PRP中的TGF-β浓度为(737.68±62.7)ng/ml。其中以900g,10min,700g,10min,这个离心速度与时间组合所得的PRP血小板

  8. Technique modification for platelet - rich plasma prepare in dogsModificação de técnica de preparo do plasma rico em plaquetas em cães

    Directory of Open Access Journals (Sweden)

    Patricia Mendes Pereira

    2012-04-01

    Full Text Available Platelet-rich plasma (PRP has many growing factors that improve tissue regeneration. The goal of this paper is to describe an innovative, quick and simple technique for preparing autogenous PRP from a small amount of blood, which allowing use in dogs. After harvesting 8ml of total blood in five dogs, PRP was obtained using an aseptic technique, which rendered a platelet count two to six times more concentrated than the previous serum platelet count, with final absolute values between 800.000 and 1.600.000/mm3. The technique can be developed quickly, without complications using simple equipments, reaching an ideal platelet concentration and keeping plasma sterility.O plasma rico em plaquetas (PRP possui diversos fatores de crescimento que auxiliam na regeneração tecidual. O presente trabalho tem como objetivo propor uma técnica para o preparo do PRP autólogo, por meio de um protocolo simples e rápido, obtido a partir de um volume pequeno de sangue total, que facilita a utilização em cães. Após a colheita de 8ml de sangue total, realizada em cinco cães, obteve-se o PRP, de maneira asséptica, com uma concentração de plaquetas de duas a seis vezes maior que a contagem sérica inicial, com valores absolutos finais de 800.000 a 1.600.000/mm³. A técnica pode ser realizada sem complicações, de maneira rápida, com poucos equipamentos, atingindo-se a concentração ideal das plaquetas e mantendo-se a esterilidade do plasma.

  9. High-throughput proteomic characterization of plasma rich in growth factors (PRGF-Endoret)-derived fibrin clot interactome.

    Science.gov (United States)

    Anitua, Eduardo; Prado, Roberto; Azkargorta, Mikel; Rodriguez-Suárez, Eva; Iloro, Ibon; Casado-Vela, Juan; Elortza, Felix; Orive, Gorka

    2015-11-01

    Plasma rich in growth factors (PRGF®-Endoret®) is an autologous technology that contains a set of proteins specifically addressed to wound healing and tissue regeneration. The scaffold formed by using this technology is a clot mainly composed of fibrin protein, forming a three-dimensional (3D) macroscopic network. This biomaterial is easily obtained by biotechnological means from blood and can be used in a range of situations to help wound healing and tissue regeneration. Although the main constituent of this clot is the fibrin scaffold, little is known about other proteins interacting in this clot that may act as adjuvants in the healing process. The aim of this study was to characterize the proteins enclosed by PRGF-Endoret scaffold, using a double-proteomic approach that combines 1D-SDS-PAGE approach followed by LC-MS/MS, and 2-DE followed by MALDI-TOF/TOF. The results presented here provide a description of the catalogue of key proteins in close contact with the fibrin scaffold. The obtained lists of proteins were grouped into families and networks according to gene ontology. Taken together, an enrichment of both proteins and protein families specifically involved in tissue regeneration and wound healing has been found.

  10. 不同浓度富血小板血浆与前交叉韧带重建后的隧道扩大%Effects of different concentrations of platelet-rich plasma on tunnel enlargement after anterior cruciate ligament reconstruction

    Institute of Scientific and Technical Information of China (English)

    李洪秋; 阿良; 赵忠海; 东黎阳

    2013-01-01

    BACKGROUND:Platelet-rich plasma contains growth factors that can promote tendon-bone healing, thereby reducing occurrence of bone tunnel enlargement after anterior cruciate ligament reconstruction;different concentrations of platelet-rich plasma have certain influence on bone tunnel enlargement, but it is not clear which concentration is optimal. OBJECTIVE:To study the effect of platelet-rich plasma on tunnel enlargement after anterior cruciate ligament reconstruction. METHODS:Bilateral anterior cruciate ligament reconstruction was conducted in 20 New Zealand white rabbits using Martinek et al’s method. Tendon graft was pretreated with 10 mg platelet-rich plasma gel on one side as an observation group. Graft on the contralateral limb was pretreated with 5 mg platelet-rich plasma gel as control group. At 12 weeks after surgery, radiological observation and measure of the width of bone tunnel were conducted. Tunnel enlargement rate was calculated. RESULTS AND CONCLUSION:At 8 and 12 weeks after anterior cruciate ligament reconstruction, the width of tunnel enlargement was less in the 10 mg-platelet-rich plasma group than that in the 5 mg-platelet-rich plasma group (P  目的:观察富血小板血浆对前交叉韧带重建后隧道扩大的影响。  方法:将20只新西兰大白兔,参照Martinek等的方法进行兔的双侧前交叉韧带重建。在一侧肢体的肌腱移植物给予10 mg富血小板血浆凝胶预处理作为观察组,对侧肢体移植物给予5 mg富血小板血浆凝胶预处理为对照组。处理后12周进行影像学观察以及测量骨隧道的宽度,计算隧道扩大率。  结果与结论:在前交叉韧带重建后的8周、12周的时间点上,10 mg富血小板血浆组隧道扩大的宽度小于5 mg富血小板血浆组(P <0.05);10 mg富血小板血浆组的腱-骨结合部位的骨密度值大于5 mg富血小板血浆组(P <0.05)。提示富血小板血浆能够促进兔前交叉韧带重建后

  11. Fibrin(ogen)olytic character of FⅡa isolated from Agkistrodon acutus venom

    Institute of Scientific and Technical Information of China (English)

    Yi-wen WANG; Xiu-xia LIANG; Jia-shu CHEN; Qi CHEN; Peng-xin QIU; Xi LIN; Guang-mei YAN

    2005-01-01

    Aim: To investigate the fibrin(ogen)olytic character of FⅡa isolated from Agkistrodon acutus venom in vitro and in vivo. Methods: 125I-labeled human plasma clot lysis was measured in vitro and rabbit carotid artery thrombosis was as an in vivo model. Results: In vitro, urokinase (UK) at 25, 35, 40, 45, 60 kU/L and FⅡa at 0.08, 0.23, 0.4, 0.5, and 0.7 g/L resulted an equivalent clot lysis (20%, 40%,50%, 60%, and 80%). UK at 25-60 kU/L induced 27.3%±3.6%, 35.2%±2.3%,39.3%±2.4%, 44.2%±4.6%, and 51.1%± 1.2% fibrinogen degradation. But FⅡa at 0.08-0.7 g/L induced 95.4%±0.3%, >95.6%, >95.6%, >95.6%, >95.6% fibrinogen degradation respectively. In vivo, UK 40 kU/kg and FⅡa 1.0 mg/kg reduced the weight of residual thrombus to 9.0±2.5 mg and 7.8±3.5 mg compared with negative control group (30.0±5.4 mg). But the fibrinogen degradation rate after UK 40 kU/kg and FⅡa 1.0 mg/kg treatment was 24.4%±6.2% and 4.1%±7.8%, respectively (P<0.05, n=6). The order of the lysis speed after UK 125 kU/L treatment was platelet poor plasma (PPP) clots>the whole blood clots>platelet rich plasma (PRP) clots.The sequence for FⅡa 0.4 g/L was PRP>PPP>whole blood clots. Conclusion: At the same percentage of clot lysis, FⅡa degraded more fibrinogen than UK did in vitro but less fibrinogen than UK did in vivo. The order of the lysis speed was PPP>whole blood clots>PRP clots for UK and PRP>PPP>whole blood clots for FⅡa.

  12. Fibrin-mediated lentivirus gene transfer: implications for lentivirus microarrays

    OpenAIRE

    Raut, Shruti; Lei, Pedro; Padmashali, Roshan; Andreadis, Stelios T.

    2010-01-01

    We employed fibrin hydrogel as bioactive matrix for lentivirus mediated gene transfer. Fibrin-mediated gene transfer was highly efficient and exhibited strong dependence on fibrinogen concentration. Efficient gene transfer was achieved with fibrinogen concentration between 3.75 – 7.5 mg/mL. Lower fibrinogen concentrations resulted in diffusion of virus out of the gel while higher concentrations led to ineffective fibrin degradation by target cells. Addition of fibrinolytic inhibitors decrease...

  13. Fibrin glue for Gundersen flap surgery

    Directory of Open Access Journals (Sweden)

    Chung HW

    2013-03-01

    Full Text Available Hsi-Wei Chung,1 Jodhbir S Mehta1–31Singapore National Eye Centre, Singapore; 2Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore; 3Department of Clinical Sciences, Duke-NUS Graduate Medical School, SingaporePurpose: To evaluate the feasibility of fibrin glue in Gundersen flap surgery.Design: Prospective case series.Study subjects: Seven eyes of seven subjects who had undergone Gundersen flap surgery from 2009 to 2011 at the Singapore National Eye Centre, Singapore.Methods: Review of case records for outcomes after Gundersen flap surgery.Main outcome measures: Surgical success was defined as achieving a stable ocular surface. Complications to be noted included flap retraction or exposure of underlying corneal surface.Results: Surgical success was achieved in all eyes with significant reduction in ocular surface inflammation. No retractions were noted and recovery was uncomplicated.Conclusion: Fibrin glue application is a viable alternative to sutures in Gundersen flap surgery. It reduces surgical downtime, gives faster ocular surface rehabilitation, and offers similar outcomes to conventional conjunctival flap surgery.Keywords: Gundersen, conjunctival flap, fibrin glue

  14. Scaffold architecture and fibrin gels promote meniscal cell proliferation

    International Nuclear Information System (INIS)

    Stability of the knee relies on the meniscus, a complex connective tissue with poor healing ability. Current meniscal tissue engineering is inadequate, as the signals for increasing meniscal cell proliferation have not been established. In this study, collagen scaffold structure, isotropic or aligned, and fibrin gel addition were tested. Metabolic activity was promoted by fibrin addition. Cellular proliferation, however, was significantly increased by both aligned architectures and fibrin addition. None of the constructs impaired collagen type I production or triggered adverse inflammatory responses. It was demonstrated that both fibrin gel addition and optimized scaffold architecture effectively promote meniscal cell proliferation

  15. Scaffold architecture and fibrin gels promote meniscal cell proliferation

    Energy Technology Data Exchange (ETDEWEB)

    Pawelec, K. M., E-mail: pawelec.km@gmail.com, E-mail: jw626@cam.ac.uk; Best, S. M.; Cameron, R. E. [Cambridge Centre for Medical Materials, Materials Science and Metallurgy Department, University of Cambridge, Cambridge CB3 0FS (United Kingdom); Wardale, R. J., E-mail: pawelec.km@gmail.com, E-mail: jw626@cam.ac.uk [Division of Trauma and Orthopaedic Surgery, Department of Surgery, University of Cambridge, Cambridge CB2 2QQ (United Kingdom)

    2015-01-01

    Stability of the knee relies on the meniscus, a complex connective tissue with poor healing ability. Current meniscal tissue engineering is inadequate, as the signals for increasing meniscal cell proliferation have not been established. In this study, collagen scaffold structure, isotropic or aligned, and fibrin gel addition were tested. Metabolic activity was promoted by fibrin addition. Cellular proliferation, however, was significantly increased by both aligned architectures and fibrin addition. None of the constructs impaired collagen type I production or triggered adverse inflammatory responses. It was demonstrated that both fibrin gel addition and optimized scaffold architecture effectively promote meniscal cell proliferation.

  16. Efficacy and safety of the use of platelet-rich plasma for sternal wound infections: a meta-analysis%富血小板血浆预防胸骨感染效果的Meta分析

    Institute of Scientific and Technical Information of China (English)

    孙斌; 冯子阳; 徐芳芳; 臧艳; 花美仙; 钱宝华

    2015-01-01

    目的 评估富血小板血浆(platelet-rich plasma,PRP)预防胸骨感染(sternal wound infection,SWI)的安全性与有效性.方法 计算机检索PubMed,EMbase,The Cochrane Library以及中国生物医学文献数据库(CBM)中胸心外科手术患者局部应用PRP预防感染的相关研究,并辅以文献追溯法查找相关文献.检索时限均从建库至2014年8月.由2名评价者按纳入与排除标准独立选择文献、提取资料并评价质量后,采用Stata12.0软件进行Meta分析.结果 最终纳入5个研究,共5 613名患者.Meta分析结果显示:与对照组相比,PRP组发生SWI和胸骨浅表切口感染(superficial sternal wound infection,SSWI)的风险分别下降了74%(RR =0.26;95%CI,0.08-0.88;P<0.05)和76%(RR=0.24;95% CI,0.10-0.61;P<0.05),差别有统计学意义;而PRP组胸骨深部切口感染(deep sternal wound infection,DSWI),二次手术以及死亡的风险与对照组的差别无统计学意义.结论 局部应用自体PRP可以降低胸心外科手术患者SWI和SSWI的风险,其确切机制以及其他临床应用有待进一步研究.

  17. The effect of platelet-rich plasma on cavernous nerve regeneration in a rat model%富血小板血浆在大鼠海绵体神经再生中的作用

    Institute of Scientific and Technical Information of China (English)

    Xie-Gang Ding; Shi-Wen Li; Xin-Min Zheng; Li-Quan Hu; Wan-Li Hu; Yi Luo

    2009-01-01

    The aim of this study was to investigate the effect of platelet-rich plasma (PRP) on cavernous nerve (CN) re-generation and functional status in a nerve-crush rat model. Twenty-four Sprague-Dawley male rats were randomly divided into three equal groups: eight had a sham operation, eight underwent bilateral nerve crushing with no fur-ther intervention and eight underwent bilateral nerve crushing with an immediate application of PRP on the site of injury. Erectile function was assessed by CN electrostimulation at 3 months and nerve regeneration was assessed by toluidine blue staining of CN and nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase staining of penile tissue. Three months after surgery, in the group that underwent bilateral nerve crushing with no further inter-vention, the functional evaluation showed a lower mean maximal intracavernous pressure (ICP) and maximal ICP per mean arterial pressure (MAP) with CN stimulation than those in the sham group. In the group with an immedi-ate application of PRP, the mean maximal ICP and maximal ICP/MAP were significantly higher than those in the injured control group. Histologically, the group with the application of PRP had more myelinated axons of CNs and more NADPH-diaphorase-positive nerve fibres than the injured control group but fewer than the sham group. These results show that the application of PRP to the site of CN-crush injury facilitates nerve regeneration and recovery of erectile function. Our research indicates that clinical application of PRP has potential repairing effect on CN and peripheral nerves.%本文探讨富血小板血浆在大鼠海绵体神经压榨损伤模型中对该神经再生和勃起功能的修复效果.24只成年雄性sD大鼠随机均分成3组:假手术组、海绵体神经压榨损伤组、损伤后富血小板血浆立即应用组.3个月后通过电刺激海绵体神经来观察其勃起功能、海绵体神经的甲苯胺蓝染色和阴茎组织的NADPH染色来

  18. 富血小板血浆对大鼠阴茎海绵体神经再生的影响%Effect of platelet rich plasma on the regeneration of cavernous nerve:experiment with rats

    Institute of Scientific and Technical Information of China (English)

    丁协刚; 李世文; 郑新民; 胡礼泉; 胡万里; 罗仪

    2008-01-01

    Objective To investigate the effect of platelet rich plasma(PRP)on the regeneration of injured cavernous nerve(CN).Methods Blood was collected from the hearts of 6 SD rata to prepare PRP.24 male adult rats were randomly divided into 3 equal groups:pure suture group undergoing bilateral CN transaction and pure suture immediately,PRP group undergoing bilateral CN transaction+suture+PRP 200 μl to the site of suture,and sham operation group.3 months later intracavernous pressure(ICP)was measured by CN electrostimulation and then samples of CN were obrained to undergo pathological examination.Results 3 months later after surgery,the ICP of the pure suture group was(46±8)cm H2O,significantly lower than that of the sham group[(109±13)cm H2O,P<0.01],and that of the PRP group was(94±13)cm H2O,significantly higher than that of the pure sutured group(P<0.01),however,still significantly lower than that of the sham operation group(P<0.05).The number of axons of CN in the PRP group was(121±16),significantly higher than that of the pure sutured group(70±14,P<0.01);however,still significantly lower than that of the sham operation group(181±21,P<0.01).Conclusion PRP can promote the regeneration of injured CN and the recovery of erectile function.%目的 探讨富血小板血浆(PRP)是否促进大鼠海绵体神经再生.方法 24只SD雄性大鼠分成假手术组、双侧海绵体神经切断后立即显微缝合组(单纯缝合组)及缝合后立即局部应用组(PfuP应用组),术后3个月通过电刺激观察海绵体内压的变化来反映其勃起功能,随后取海绵体神经干进行甲苯胺蓝染色,观察轴突的变化来反映神经再生情况.结果 术后3个月,海绵体神经单纯缝合组最大海绵体内压为(46±8)cmH2O,明显低于假手术组(109±13)cmH2O(P<0.01);而PRP应用组为(94±13)em H2O,明显高于单纯缝合组(P<0.01).甲苯胺蓝染色PRP应用组轴突个数为(121±16),明显高于单纯缝合组(70±14)(P<0

  19. Estudo sobre a associação de cerâmica a plasma rico em plaquetas na coluna vertebral de ratos Study about fusion using ceramic with platelet-rich plasma in the spine of rats

    Directory of Open Access Journals (Sweden)

    Rita Delgado

    2009-01-01

    Full Text Available OBJETIVO: Analisar o efeito osteoindutor dos fatores de crescimento contidos no plasma rico em plaquetas (PRP quando associados a cerâmicas no processo de fusão na coluna vertebral de ratos. MATERIAL E MÉTODO: Laminectomia lombar foi realizada em 16 ratos Lewis isogênicos para implantar cerâmica. O PRP foi preparado no ato operatório a partir do sangue de outras duas cobaias.os grupos estudo e controle foram estabelecidos por randomização, recebendo o grupo estudo cerâmica associada a PRP e o controle apenas cerâmica. Os animais foram sacrificados após 10 semanas e as colunas enviadas para análise histopatológica. RESULTADOS: Intensa atividade osteoblástica e osteoclástica e completa reabsorção da cerâmica foram achados no grupo estudo. No grupo controle havia ilhotas de osso em meio a tecido fibroso e cerâmica não reabsorvida. Discussão: Os fatores de crescimento liberados pelas plaquetas agem ligando-se à superfície de osteoblastos e fibroblastos, estimulando a síntese de colágeno para formação de matriz óssea. macrófagos ativados continuam secretando os fatores de crescimento e estimulando a osteogênese. CONCLUSÃO: O uso de PRP associado a cerâmica mostrou maior atividade osteoblástica e osteoclástica e completa reabsorção da cerâmica em relação à enxertia da mesma isoladamente na coluna vertebral de ratos.OBJECTIVE: To assess the osteoinductive action of platelet-rich plasma when associated to ceramics in the spine of rats. MATERIAL AND METHOD: Laminectomy was performed in 16 isogenic Lewis rats for posterior ceramic grafting. PRP was prepared intraoperatively using blood collected from two other rats. Study and control groups were set by randomization, with the study group receiving ceramics associated to PRP, and the control group receiving only ceramics. The animals were sacrificed for histopathological analysis after 10 weeks. RESULTS: Strong osteoblastic and osteoclastic activity and full re

  20. Efeito do Plasma Rico em Plaquetas na apoptose pós-traumática de condrócitos Effect of Platelet-Rich Plasma on impact-induced chondrocyte apoptosis

    Directory of Open Access Journals (Sweden)

    Márcia Uchôa de Rezende

    2011-04-01

    Full Text Available OBJETIVO: Avaliar se a injeção intra-articular de Plasma rico em plaquetas (PRP pode reduzir a apoptose pós-traumática de condrócitos. MÉTODOS: Foi desenvolvido um estudo experimental duplo-cego com quatro joelhos de coelhos adultos. Após a anestesia, os animais foram submetidos à contusão padronizada dos joelhos. Depois foi injetado 1ml de PRP humano nos dois joelhos esquerdos e 1ml de solução fisiológica (SF nos dois joelhos direitos. Os dois coelhos foram mantidos no mesmo ambiente sob controle de temperatura, de atividades diárias e de alimentação. A eutanásia dos animais ocorreu dez dias após a intervenção e foram realizadas biópsias da cartilagem de cada joelho. As peças foram preparadas para análise em microscopia eletrônica (ME. RESULTADOS: Quatro preparados para ME foram obtidos, cada um correspondendo a um joelho. Os joelhos-PRP apresentaram as taxas de apoptose de 47,62% (50/105 e de 48,36% (59/122, respectivamente. Nos joelhos-SF as taxas de apoptose foram, respectivamente, 56,67% (17/30 e 70,40% (88/125. A diferença do índice de apoptose nos joelhos-PRP (48,02% e nos joelhos-SF (67,74% foi significante (pOBJECTIVE: To evaluate if the injection of intra-articular platelet-rich plasma (PRP can reduce impact-induced chondrocyte apoptosis. METHODS: A double-blind experimental study was developed in four knees of two adult rabbits. Each knee was injured after anesthesia. Subsequently, 1ml PRP was injected in the right knees and 1ml of normal saline (NS in the left knees. The animals were euthanized ten days after the intervention. All cartilage was removed from the 4 knees and prepared for analysis in electron microscopy (EM. RESULTS: Four EM samples were obtained. The PRP-injected knees showed apoptosis rates of 47,62% (50/105 and 48,36% (59/122, respectively. NS-injected knees showed 56.67% (17/30 and 70.40% (88/125 of apoptosis. PRP-injected knees had statistically significant less apoptosis (48.02% than NS

  1. Autologous Fat Injection for Augmented Mammoplasty

    International Nuclear Information System (INIS)

    Autologous fat injection is one of the methods utilized for augmented mammoplasty methods. In this surgical procedure, the fat for transfer is obtained from the donor site of the patient's own body by liposuction and the fat is then injected into the breast. We report here cases of three patients who underwent autologous fat injection. Two of the patients had palpable masses that were present after surgery. The serial imaging findings and surgical method of autologous fat transfer are demonstrated