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Sample records for autologous fibrin glue

  1. Use of autologous fibrin glue in dermatologic surgery: application of skin graft and second intention healing

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    Aparecida Machado de Moraes

    Full Text Available OBJECTIVE: To evaluate the efficiency of biological sealant, an autologous fibrin glue, in dermatological surgery. DESIGN: Randomized clinical trial. SETTING: The Dermatology Service of Hospital das Clínicas, Universidade de Campinas (UNICAMP, referral center. PATIENTS: 14 patients with malign epithelial cutaneoos tumors participated in the evaluation, each having two tumors, generally facial and symmetrical, in order to perform a comparative evaluation on the same individual. PROCEDURES: The glue was prepared beforehand with a sample of autologous blood. Surgical extirpation of the tumor was followed by grafts or second intention healing. OUTCOMES: The efficiency of the sealant was then evaluated in relation to hemostasis, adhesion, surgical time and evolution of the granulation tissue, clinically and histologically. RESULTS: Immediate hemostasis and graft adhesion, with a significant reduction of surgical time, and in the open wounds there was immediate hemostasis and a clinical increase in granulation tissue, but with no histological differences among the groups on the 7th day. CONCLUSION: It is an adjuvant resource in skin cancer surgery.

  2. A Novel Method for Iatrogenic Vesicovaginal Fistula Treatment: Autologous Platelet Rich Plasma Injection and Platelet Rich Fibrin Glue Interposition

    OpenAIRE

    2014-01-01

    Introduction: Vesicovaginal fistula remains a challenge in surgical therapy. In this study autologous platelet rich plasma and platelet rich fibrin glue were used as a minimally invasive approach for vesicovaginal fistula closure. Materials and Methods: Data including age, parity, ICIQ-UI (International Consultation on Incontinence Questionnaire-urinary incontinence), ICIQ-QOL (International Consultation on Incontinence Questionnaire-quality of life), duration of leakage, fistula d...

  3. FIBRIN GLUE DAN APLIKASINYA

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    Agi Harliani S

    2015-08-01

    Full Text Available Fibrin Tissue Adhesive (FTA, Fibrin Sealant (FS or Fibrin Glue (FG are names given to a group of product that lead to the formation of fibrin clot at the site of application. Fibrin Glue represents a new revolution for local haemostatic, which produced by based on the understanding about blood coagulation process. The mechanism of FG mimics the last stage of blood coagulation process. Haemophilia, is a congenital inherited bleeding disorder, characterized by repeated bleeding episodes. The basic pathology is deficiency of factor VIII (hemophilia A or factor IX (hemophilia B. At bleeding episodes, hemophilia patients need replacement therapy. Hemophilia patients need transfusion of cryoprecipitate, Fresh Frozen Plasma (FFP or factor concentrate as replacement therapy. Oral surgery, dental extraction, circumcision, and orthopedic operations are the most important indications for fibrin glue in hemophilia care. As haemostatic local, FG minimizes bleeding, reducing the need of transfusion or factor concentrate, reducing the complication of transfusion, hospitalization and cost.

  4. Treatment of Hypertrophic Scar in Human with Autologous Transplantation of Cultured Keratinocytes and Fibroblasts along with Fibrin Glue

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    Ehsan Taghiabadi

    2015-04-01

    Full Text Available Objective: Hypertrophic scar involves excessive amounts of collagen in dermal layer and may be painful. Nowadays, we can’t be sure about effectiveness of procedure for hypertrophic scar management. The application of stem cells with natural scaffold has been the best option for treatment of burn wounds and skin defect, in recent decades. Fibrin glue (FG was among the first of the natural biomaterials applied to enhance skin deformity in burn patients. This study aimed to identify an efficient, minimally invasive and economical transplantation procedure using novel FG from human cord blood for treatment of hypertrophic scar and regulation collagen synthesis. Materials and Methods: In this case series study, eight patients were selected with hypertrophic scar due to full-thickness burns. Human keratinocytes and fibroblasts derived from adult skin donors were isolated and cultured. They were tested for the expression of cytokeratin 14 and vimentin using immunocytochemistry. FG was prepared from pooled cord blood. Hypertrophic scars were extensively excised then grafted by simply placing the sheet of FG containing autologous fibroblast and keratinocytes. Histological analyses were performed using Hematoxylin and eosin (H&E and Masson’s Trichrome (MT staining of the biopsies after 8 weeks. Results: Cultured keratinocytes showed a high level of cytokeratin 14 expression and also fibroblasts showed a high level of vimentin. Histological analyses of skin biopsies after 8 weeks of transplantation revealed re-epithelialization with reduction of hypertrophic scars in 2 patients. Conclusion: These results suggest may be the use of FG from cord blood, which is not more efficient than previous biological transporters and increasing hypertrophic scar relapse, but could lead to decrease pain rate.

  5. [Anaphylactic shock caused by fibrin glue].

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    Orsel, I; Guillaume, A; Feiss, P

    1997-01-01

    We observed a case of anaphylactic shock in a 68-year-old woman after a nasal intramucosal injection of fibrin glue for telangiectasies therapy. The tests showed an allergy to aprotinin contained in the glue. In the previous years, glue and aprotinin had been administered to the patient several times for nasal bleeding.

  6. Evolution of fibrin glue applicators.

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    Marx, Gerard

    2003-10-01

    Fibrin glue (FG) is used worldwide as a potent surgical tool, which establishes hemostasis in wounds and also bonds tissue. The standard FG applicator is based on a dual-syringe system. This review, based mainly on the patent literature, describes the development of the quasi-standard dual syringe system as well as the rise of other FG applicator designs based on mechanical force (ratchet systems), Bernoulli gas flow, positive gas pressure, or electro-servo devices. The packaging of commercial FG components is reviewed within the context of "loading" the FG applicators and the need to minimize the number of needles required to access the packaged (vials) components. Parameters such as internal clogging, homogeneity of spray, the requirement for gas or vacuum house lines, the number of parts that must be handled, and the time required to assemble the applicator, load it, and have it ready for use are also discussed. A rating system is proposed that permits one to use such parameters to rank the various applicator designs, relative to the dual-syringe system. Hopefully, this review will stimulate the design of better FG applicators and packaging required for elective surgery, emergency treatments, and tissue engineering in the 21st century.

  7. Application of fibrin glue on endoscopic liposuction

    Institute of Scientific and Technical Information of China (English)

    CHEN Zhong-cun; YANG Jia-feng; CHEN Qian-qian

    2001-01-01

    @@ OBJECTIVE To deserve the effects of FG (Fibrin Glue)on endoscopic liposuction. Methods:From Oct. 1998 to Aug. 2000 forty cases were undergoing for the liposuction. Atthe aids of tumescent teachnique, endoscopy and FG were used, of which 20 casesoperation on their abdominal walls, 8 cases upper abdomen walls and 12 cases ownerabdominal.

  8. [Application of fibrin glue in facial nerve repair].

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    Wang, Qinying; Hua, Qingquan; Wang, Shenqing

    2007-06-01

    This animal experiment was aimed to apply fibrin in facial nerve repair and to quest for technical improvements in facial surgery. In each of 15 healthy large ear white rabbits, a unilateral 5 mm intratemporal facial nerve gap was created, the proximal and distal stumps were inserted into chitin tube, 1 ml autologous fibrin glue was applied around the anastomotic zone, and no suture was employed. At 3 months and 5 months after opertion, electrophysioligical study was performed. Compared with normal nerves, the regenerating nerves in both the chitin tube bridged group and the perineurium suture group had longer incubation period, lower amplitude, slower nerve-muscle conduction velocity at 3 months postoperatively. The differences were distinctly significant (P < 0.01). Although being decreased at 5 months after operation, the differences were still statistically significant (P < 0.05). There were no significant differences between the chitin tube bridged group and perineurium suture group at 3 months and 5 months, respectively. The study suggests that facial nerve repair using fibrin glue and chitin tube has the advantages of being easier,faster and more stable.

  9. Effect of collagen sponge and fibrin glue on bone repair

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    Thiago de Santana SANTOS

    2015-12-01

    Full Text Available ABSTRACT The ability of hemostatic agents to promote bone repair has been investigated using in vitro and in vivo models but, up to now, the results are inconclusive. Objective In this context, the aim of this study was to compare the potential of bone repair of collagen sponge with fibrin glue in a rat calvarial defect model. Material and Methods Defects of 5 mm in diameter were created in rat calvariae and treated with either collagen sponge or fibrin glue; untreated defects were used as control. At 4 and 8 weeks, histological analysis and micro-CT-based histomorphometry were carried out and data were compared by two-way ANOVA followed by Student-Newman-Keuls test when appropriated (p≤0.05. Results Three-dimensional reconstructions showed increased bone formation in defects treated with either collagen sponge or fibrin glue compared with untreated defects, which was confirmed by the histological analysis. Morphometric parameters indicated the progression of bone formation from 4 to 8 weeks. Additionally, fibrin glue displayed slightly higher bone formation rate when compared with collagen sponge. Conclusion Our results have shown the benefits of using collagen sponge and fibrin glue to promote new bone formation in rat calvarial bone defects, the latter being discreetly more advantageous.

  10. Complex reconstructive surgery following removal of extra-intracranial meningiomas, including the use of autologous fibrin glue and a pedicled muscle flap

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    Antonella Giugno, M.D.

    2014-12-01

    Conclusions: Reparation of CSF fistulas that arise after meningioma surgery can require a complex reconstructive surgery of the superficial layers; when cranioplasty is not feasible or indicated, a meticulous reconstruction of the extracranial soft tissues is possible also by using vascularized autologous distal muscular tissue, with close interdisciplinary cooperation.

  11. Histological effects of fibrin glue on nervous tissue: a safety study in rats.

    NARCIS (Netherlands)

    Vries, J. de; Menovsky, T.; Gulik, S. van; Wesseling, P.

    2002-01-01

    BACKGROUND: Little is known of the histologic effects of fibrin glue on normal nervous tissue. To verify the safety of intracranial application of fibrin glue, we investigated the histologic effects of fibrin glue on brain tissue and intracranial nerves of rats. METHODS: In Group I (n =12), bifronta

  12. RECONSTRUCTION OF THE SELLAR FLOOR FOLLOWING TRANSSPHE-NOIDAL SURGERY USING GELATIN FOAM AND FIBRIN GLUE

    Institute of Scientific and Technical Information of China (English)

    Jian Yin; Chang-bao Su; Zhi-qin Xu; Xue-wei Xia; Fei Song

    2005-01-01

    Objective To introduce a new principle of sellar reconstruction and to evaluate the effectiveness of absorbable gelatin foam and fibrin glue for sellar reconstruction. Methods A total of 176 consecutive patients who underwent surgery for pituitary adenomas, cysts, chordomas, or subdiaphragmatic craniopharyngiomas in the sella turcica between January 2001 and April 2003 at Peking Union Medical College Hospital were enrolled. Different techniques of sellar closure and indications for each specific condition were retrospectively reviewed. Results Seventy-seven (43.7%) patients developed a visible cerebrospinal fluid (CSF) leakage during surgery. Intra operative CSF leakage were repaired simply with gelatin foam and fibrin glue in 62 (35.2%) patients, and with autologous fat graft and sellar floor reconstruction in 15 (8.5%) patients. Postoperative CSF rhinorrhea occurred only in 1 case. There were no visual deterioration, allergic rhinitis, meningitis, pneumocranium, granulomas, or other complications associated with the reconstruction procedure. Conclusion The procedure of using gelatin foam and fibrin glue and principle of cranial base reconstruction is safe and effective in preventing postoperative complications following transsphenoidal surgery.

  13. Preparation of two component Fibrin Glue and its clinical evaluation in skin grafts and flaps

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    Jain P

    2003-01-01

    Full Text Available Tissue adhesive is one of the alternative to conventional suturing and has some added advantages. Fibrin glue has been used in obtaining haemostasis following trauma to spleen and liver. It has also been used in repair of dural tear and bronchial fistula. Fibrin glue is a biological tissue adhesive based on the final stage of coagulation wherein. Thrombin acting on fibrinogen converts it into fibrin. Thus, it has two components, one is fibrinogen and another is thrombin. We have prepared both components of fibrin glue. Fibrinogen was obtained from patient's own blood and thrombin from fresh frozen plasma of screened healthy donor. The glue was used in 20 cases requiring skin graft or flap. The results were compared with conventional suturing method. Use of the fibrin glue is simple, safe, cost effective, and rapid technique to fix the skin grafts and flaps with avoidance of peroperative bleeding and postoperative collection. It also has better overall results.

  14. Transplante autólogo de conjuntiva com uso de cola de fibrina em pterígios primários Conjunctival autologous transplantation using fibrin glue in primary pterygium

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    Ramon Coral-Ghanem

    2010-08-01

    complicações. Apesar de resultar em menor taxa de recidiva conjuntival, esta técnica não parece diminuir significativamente a incidência de recidiva corneana.PURPOSE: To assess the rate of recurrence and complications after primary pterygium removal with conjunctival autograft using fibrin tissue adhesive. Secondarily, to compare these results with a retrospective series that underwent the same surgery using sutures. METHODS: A hundred and six eyes of 100 patients who underwent surgery with fibrin glue were prospectively studied, while 58 eyes of 51 patients who underwent surgery with sutures were retrospectively evaluated. Data included: age, gender, eye involved, location, grade and pterygium invasion and post-operative complications. Patients were followed for at least 5 months. RESULTS: Among 106 eyes undergoing surgery with fibrin glue, 12 (11.3% had recurrence, including 4 (3.8% conjunctival and 8 (7.5% corneal. Mean age was statistically inferior in the recurrence cases compared to others (mean of 34.6 vs. 43.7; P=0.033. The time for recurrence ranged from 1.6 to 13.1 months (mean of 4.4. Among the corneal recurrences, 2 eyes had invasion beyond the limbus of up to 0.5 mm and 6 eyes from 0.6 to 1.0 mm. No eye underwent reoperation. Other complications included: transitory granuloma formation in 3 cases, partial graft detachment or slippage in 3 and dellen in 1. In the suture group, 15 eyes (25.9% had recurrence, including 8 conjunctival (13.8% and 7 (12.1% corneal. The time for recurrence ranged from 0.7 to 9.7 months (mean 4.5 months. The conjunctival recurrence rate was statistically inferior in the glue group compared to the suture (P=0.023, however no statistically significant difference was observed in relation to corneal recurrence (P=0.232. CONCLUSION: The use of fibrin glue as an alternative to sutures in pterygium surgery was associated with good results and few complications. Despite a decrease in conjunctival recurrence rates, this technique does not

  15. Fibrin glue in the treatment of anal fistula: a systematic review

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    Covarelli Piero

    2009-11-01

    Full Text Available Abstract Background New sphincter-saving approaches have been applied in the treatment of perianal fistula in order to avoid the risk of fecal incontinence. Among them, the fibrin glue technique is popular because of its simplicity and repeatability. The aim of this review is to compare the fibrin glue application to surgery alone, considering the healing and complication rates. Methods We performed a systematic review searching for published randomized and controlled clinical trials without any language restriction by using electronic databases. All these studies were assessed as to whether they compared conventional surgical treatment versus fibrin glue treatment in patients with anal fistulas, in order to establish both the efficacy and safety of each treatment. We used Review Manager 5 to conduct the review. Results The healing rate is higher in those patients who underwent the conventional surgical treatment (P = 0,68, although the treatment with fibrin glue gives no evidence of anal incontinence (P = 0,08. Furthermore two subgroup analyses were performed: fibrin glue in combination with intra-adhesive antibiotics versus fibrin glue alone and anal fistula plug versus fibrin glue. In the first subgroup there were not differences in healing (P = 0,65. Whereas in the second subgroup analysis the healing rate is statistically significant for the patients who underwent the anal fistula plug treatment instead of the fibrin glue treatment (P = 0,02. Conclusion In literature there are only two randomized controlled trials comparing the conventional surgical management versus the fibrin glue treatment in patients with anal fistulas. Although from our statistical analysis we cannot find any statistically significant result, the healing rate remains higher in patients who underwent the conventional surgical treatment (P = 0,68, and the anal incontinence rate is very low in the fibrin glue treatment group (P = 0,08. Anyway the limited collected data do

  16. Fibrin glue mixed with platelet-rich fibrin as a scaffold seeded with dental bud cells for tooth regeneration.

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    Yang, Kai-Chiang; Wang, Chun-Hao; Chang, Hao-Hueng; Chan, Wing P; Chi, Chau-Hwa; Kuo, Tzong-Fu

    2012-11-01

    Odontogenesis is a complex process with a series of epithelial-mesenchymal interactions and odontogenic molecular cascades. In tissue engineering of teeth from stem cells, platelet-rich fibrin (PRF), which is rich in growth factors and cytokines, may improve regeneration. Accordingly, PRF was added into fibrin glue to enrich the microenvironment with growth factors. Unerupted second molar tooth buds were harvested from miniature swine and cultured in vitro for 3 weeks to obtain dental bud cells (DBCs). Whole blood was collected for the preparation of PRF and fibrin glue before surgery. DBCs were suspended in fibrin glue and then enclosed with PRF, and the DBC-fibrin glue-PRF composite was autografted back into the original alveolar sockets. Radiographic and histological examinations were used to identify the regenerated tooth structure 36 weeks after implantation. Immunohistochemical staining was used to detect proteins specific to tooth regeneration. One pig developed a complete tooth with crown, root, pulp, enamel, dentin, odontoblast, cementum, blood vessels, and periodontal ligaments in indiscriminate shape. Another animal had an unerupted tooth that expressed cytokeratin 14, dentin matrix protein-1, vascular endothelial growth factor, and osteopontin. This study demonstrated, using autogenic cell transplantation in a porcine model, that DBCs seeded into fibrin glue-PRF could regenerate a complete tooth.

  17. Fibrin glue mixed with gelatin/hyaluronic acid/chondroitin-6-sulfate tri-copolymer for articular cartilage tissue engineering: the results of real-time polymerase chain reaction.

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    Chou, Cheng-Hung; Cheng, Winston T K; Kuo, Tzong-Fu; Sun, Jui-Sheng; Lin, Feng-Huei; Tsai, Jui-Che

    2007-09-01

    Autologous fibrin glue has been demonstrated as a potential scaffold with very good biocompatibility for neocartilage formation. However, fibrin glue has been reported not to provide enough mechanical strength, but with many growth factors to interfere the tissue growth. Gelatin/hyaluronic acid/chondroitin-6-sulfate (GHC6S) tri-copolymer sponge has been prepared as scaffold for cartilage tissue engineering and showed very good results, but problems of cell seeding and cell distribution troubled the researchers. In this study, GHC6S particles would be added into the fibrin glue to provide better mechanical strength, better cell distribution, and easier cell seeding, which would be expected to improve cartilage regeneration in vitro. Porcine cryo-precipitated fibrinogen and thrombin prepared from prothrombin activated by 10% CaCl(2) solution were used in two groups. One is the fibrin glue group in which porcine chondrocytes were mixed with thrombin-fibrinogen solution, which was then converted into fibrin glue. The other is GHC6S-fibrin glue in which GHC6S particles were added into the thrombin-fibrinogen solution with porcine chondrocytes. After culturing for 1-2 weeks, the chondrocytes cultured in GHC6S-fibrin glue showed a round shape with distinct lacuna structure and showed positive in S-100 protein immunohistochemical stain. The related gene expressions of tissue inhibitor of metalloproteinases-1, matrix metalloproteinase-2, MT1-MMP, aggrecan, decorin, type I, II, X collagen, interleukin-1 beta, transforming growth factor-beta 1 (TGF-beta1), and Fas-associating death domain were checked by real-time PCR. The results indicated that the chondrocytes cultured in GHC6S-fibrin glue would effectively promote extracellular matrix (ECM) secretion and inhibit ECM degradation. The evidence could support that GHC6S-fibrin glue would be a promising scaffold for articular cartilage tissue engineering.

  18. Alignment of the Fibrin Network Within an Autologous Plasma Clot.

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    Gessmann, Jan; Seybold, Dominik; Peter, Elvira; Schildhauer, Thomas Armin; Köller, Manfred

    2016-01-01

    Autologous plasma clots with longitudinally aligned fibrin fibers could serve as a scaffold for longitudinal axonal regrowth in cases of traumatic peripheral nerve injuries. Three different techniques for assembling longitudinally oriented fibrin fibers during the fibrin polymerization process were investigated as follows: fiber alignment was induced by the application of either a magnetic field or-as a novel approach-electric field or by the induction of orientated flow. Fiber alignment was characterized by scanning electron microscopy analysis followed by image processing using fast Fourier transformation (FFT). Besides FFT output images, area xmin to xmax, as well as full width at half maximum (FWHM) of the FFT graph plot peaks, was calculated to determine the relative degree of fiber alignment. In addition, fluorescently labeled human fibrinogen and mesenchymal stem cells (MSCs) were used to visualize fibrin and cell orientation in aligned and nonaligned plasma clots. Varying degrees of fiber alignment were achieved by the three different methods, with the electric field application producing the highest degree of fiber alignment. The embedded MSCs showed a longitudinal orientation in the electric field-aligned plasma clots. The key feature of this study is the ability to produce autologous plasma clots with aligned fibrin fibers using physical techniques. This orientated internal structure of an autologous biomaterial is promising for distinct therapeutic applications, such as a guiding structure for cell migration and growth dynamics.

  19. Microvascular anastomosis using fibrin glue and venous cuff in rat carotid artery.

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    Sacak, Bulent; Tosun, Ugur; Egemen, Onur; Sakiz, Damlanur; Ugurlu, Kemal

    2015-04-01

    Conventional anastomosis with interrupted sutures can be time-consuming, can cause vessel narrowing, and can lead to thrombosis at the site of repair. The amount of suture material inside the lumen can impair the endothelium of the vessel, triggering thrombosis. In microsurgery, fibrin sealants have the potential beneficial effects of reducing anastomosis time and promoting accurate haemostasis at the anastomotic site. However, there has been a general reluctance to use fibrin glue for microvascular anastomoses because the fibrin polymer is highly thrombogenic and may not provide adequate strength. To overcome these problems, a novel technique was defined for microvascular anastomosis with fibrin glue and a venous cuff. Sixty-four rats in two groups are included in the study. In the experimental group (n = 32), end-to-end arterial anastomosis was performed with two stay sutures, fibrin glue, and a venous cuff. In the control group (n = 32), conventional end-to-end arterial anastomosis was performed. Fibrin glue assisted anastomosis with a venous cuff took less time, caused less bleeding at the anastomotic site, and achieved a patency rate comparable to that provided by the conventional technique. Fibrin sealant assisted microvascular anastomosis with venous cuff is a rapid, easy, and reliable technique compared to the end-to-end arterial anastomosis.

  20. Fibrin glue as a protective tool for microanastomoses in limb reconstructive surgery

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    Langer, Stefan

    2015-12-01

    Full Text Available Aim: Fibrin glue becomes a more and more routinely used tool for stabilization of microanastomoses and nerve repair. This paper summarizes the technical properties and advantages of its use in a wide variety of microsurgical contexts, and includes an exemplary limb reconstructive case.Patients and methods: A total of 131 patients who had undergone elective and emergency microsurgery mainly of the limbs were retrospectively analyzed, as was the use of free flaps.Results: The use of fibrin glue allows for proper positioning of anastomoses and repaired nerves. No torsion of the pedicle could be seen. The flap survival rated >94%. The fibrin glue could stay in place in >99%. In the rare case of revision, the fibrin glue could easily be removed without damaging the region of the microanastomosis.Conclusion: Fibrin glue should not be used to repair insufficient, i.e., leaking anastomoses, but it does protect the site of anastomosis from tissue and fluid pressure. It prevents the pedickle from torsion and its use facilitates relocation of the microanastomoses in cases of revision surgery.

  1. Application of Fibrin Glue Sealant After Hepatectomy Does Not Seem Justified

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    Figueras, Juan; Llado, Laura; Miro, Mónica; Ramos, Emilio; Torras, Jaume; Fabregat, Juan; Serrano, Teresa

    2007-01-01

    Objective: To evaluate the efficacy, amount of hemorrhage, biliary leakage, complications, and postoperative evolution after fibrin glue sealant application in patients undergoing liver resection. Summary Background Data: Fibrin sealants have become popular as a means of improving perioperative hemostasis and reducing biliary leakage after liver surgery. However, trials regarding its use in liver surgery remain limited and of poor methodologic quality. Patients and Methods: A total of 300 patients undergoing hepatic resection were randomly assigned to fibrin glue application or control groups. Characteristics and debit of drainage and postoperative complications were evaluated. The amount of blood loss, measurements of hematologic parameters liver test, and postoperative evolution (particularly involving biliary fistula and morbidity) was also recorded. Results: Postoperatively, no differences were observed in the amount of transfusion (0.15 ± 0.66 vs. 0.17 ± 0.63 PRCU; P = 0.7234) or in the patients that required transfusion (18% vs. 12%; P = 0.2), respectively, for the fibrin glue or control group. There were no differences in overall drainage volumes (1180 ± 2528 vs. 960 ± 1253 mL) or in days of postoperative drainage (7.9 ± 5 vs. 7.1 ± 4.7). Incidence of biliary fistula was similar in the fibrin glue and control groups, (10% vs. 11%). There were no differences regarding postoperative morbidity between groups (23% vs. 23%; P = 1). Conclusions: Application of fibrin sealant in the raw surface of the liver does not seem justified. Blood loss, transfusion, incidence of biliary fistula, and outcome are comparable to patients without fibrin glue. Therefore, discontinuation of routine use of fibrin sealant would result in significant cost saving. PMID:17414601

  2. Determination of fibrin glue with antibiotics on collagen production in colon anastomosis

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    Stanojković Zoran

    2008-01-01

    Full Text Available Background/Aim. Fibrin glue is used as a matrix for local application of antibiotics. The aim of this study was to determine whether application of fibrin glue in combination with antibiotics can strengthen collagen production, prevent dehiscence of colon anastomoses due to infection, and reduce frequency of mortality and morbidity comparing to the control group and the group with fibrin glue application. Methods. The adult male Wistar rats divided into three groups were used in the experiment. The group 1 was the control one (after partial colon resection, colonic anastomoses performed were not treated, while to the group 2 and the group 3 were applied fibrin glue and fibrin glue with antibiotics (clindamycin and ceftriaxon on the site of anastomoses, respectively. Quality of colonic anastomoses were estimated by means of determination of collagen (L-hydroxyproline amount in the collon wall with anastomoses and histological analysis of this colon segment using light and electronic microscope on the days 5, 7 and 13 postoperatively. Results. The highest morbidity rate was registered in the group 1 (30%, then in the group 2 (13.3% and the lowest one in the group 3 (3.33%; p < 0,05 vs group 1. Mortality rate was significantly higher in the group 1 than in the group 3 (20% and 0%, respectively; p < 0,05. In the postoperative course, the highest concentrations of collagen in the colon wall on the site of anastomoses, which was confirmed by both light and electronic microscopy, were found in the group 3. Conclusion. The application of fibrin glue with antibiotics on colon anastomoses reduces the number of dehiscence, provides good mechanical protection and shorten the time of anastomoses healing.

  3. Histological assessment of titanium and polypropylene fiber mesh implantation with and without fibrin tissue glue

    NARCIS (Netherlands)

    Hallers, E.J.O. ten; Jansen, J.A.; Marres, H.A.M.; Rakhorst, G.; Verkerke, G.J.

    2007-01-01

    Polypropylene (PP) and titanium (Ti) meshes are well-known surgical implants that provoke a relative low foreign body reaction. Firm stabilization of the implant is important to prevent migration and subsequent failure of the operation. Fibrin tissue glues are commercially available adhesives and ar

  4. Fibrin glue used successfully in peritoneal dialysis catheter leakage in children.

    NARCIS (Netherlands)

    Rusthoven, E.; Kar, N.C.A.J. van de; Monnens, L.A.H.; Schröder, C.H.

    2004-01-01

    BACKGROUND: Acute renal failure in infants and small children is generally treated with peritoneal dialysis (PD). Dialysis has to be started immediately after catheter implantation. Early dialysate leakage can complicate the effectiveness of dialysis. Fibrin glue applied to the external part of the

  5. Application of fibrin glue to damaged bladder mucosa in a case of BK viral hemorrhagic cystitis.

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    Purves, J Todd; Graham, Michael L; Ramakumar, Sanjay

    2005-09-01

    BK virus is a common cause of severe hemorrhagic cystitis refractory to standard treatment. We describe a technique to achieve hemostasis after failed conservative therapy using fibrin glue applied suprapubically while visualizing and insufflating the bladder through a cystoscope. Long-term hemostasis was achieved using this novel procedure.

  6. Combined treatment of iatrogenic rectourethral fistula with endoscopic fibrin glue application and clipping.

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    Dolay, Kemal; Aras, Bekir; Tuğcu, Volkan; Ozbay, Bedi; Aygün, Erşan; Taşçi, Ali Ihsan

    2007-04-01

    Rectourethral fistula (RUF) formation is a rare complication of prostatic surgery and other pelvic surgical procedures. The results of operations to correct RUF are not always satisfying, with a particular risk of recurrent breakdown or stricture formation at the site of the urethral closure. We present a case of a small RUF treated videoendoscopically with fibrin-glue application combined with endoscopic clipping.

  7. Perivenous application of fibrin glue prevents the early injury of jugular vein graft to arterial circulation in rabbits

    Institute of Scientific and Technical Information of China (English)

    WAN Li; LI Dian-yuan; YANG Bing; WU Qing-yu

    2006-01-01

    Background Placement of an external support has been reported to prevent intimal hyperplasia of vein grafts. However, it is limited by potential complications. In the present study, we investigated the effect of fibrin glue on preventing vein graft failure as perivenous application. Methods Twenty-four rabbits were divided into non-supported group (n=12) and fibrin glue group (n=12). All animals underwent unilateral jugular vein into common carotid artery interposition grafting and then fibrin glue was applied as perivenous support. Samples of tissues were harvested after 4 weeks. Results The vein grafts with fibrin glue demonstrated a statistically significant decrease in proliferating cell nuclear antigen in the medial/intimal region [13.38% (11.26%-15.11%)] compared with non-supported vein grafts [31.22% (27.15%-35.98%)] (P<0.001). Light microscopy showed remarkable attenuation of endothelial cell loss and numerous microvessels in neoadventitia in the fibrin glue group compared with the non-supported group. The smooth muscle cells migrated into adventitia significantly in fibrin glue group, whereas the smooth muscle cells migrated into intima in non-supported group.Conclusion Perivenous support of vein graft with fibrin glue in vivo can attenuate the severe injury encountered in the non-supported vein grafts exposed to artery.

  8. Fibrin glue application in the management of refractory chylous ascites in children.

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    Zeidan, S; Delarue, A; Rome, A; Roquelaure, B

    2008-04-01

    The purpose of this retrospective review of the charts of 6 children who underwent surgical treatment of chylous ascites refractory to conservative measures between 1993 and 2006 was to evaluate the efficiency of fibrin glue application for control of lymph leakage. Five children had postoperative chylous ascites (neuroblastoma, 4; cystic lymphangioma, 1) and 1 had a congenital malformation. Surgical exploration revealed large areas of diffuse lymphatic leakage in all of the patients. Lymphatic fistula was not identified intraoperatively in any patient. Ingestion of lipophilic dye in a concentrated fatty meal was not helpful in locating a lymph fistula. Absorbable mesh was used in association with glue application in the last 3 patients treated. Control of ascites was achieved immediately in 2 patients and within 3 weeks in 2 patients. Repeat surgery was required in the remaining 2 patients. The mean follow-up time was 4.3 years. One patient died of tumor recurrence 12 months after surgical treatment without relapse of the ascites. Two mild late recurrences were observed at 6 and 11 months after surgery and were managed conservatively. The findings of this study show that fibrin glue application on absorbable mesh after dissection of the leakage zones is easy, safe, and effective. We recommend that surgery with glue application be repeated until control of ascites is achieved. We suggest fibrin glue application as a preventive measure against postoperative chylous ascites.

  9. Clinical and morphological evaluation of snake venom derived fibrin glue on the tendon healing in dogs

    Directory of Open Access Journals (Sweden)

    G. C. Ferraro

    2005-12-01

    Full Text Available The aim of this study was to evaluate the effect of snake venom derived fibrin glue on the healing of the deep digital flexor tendon, during three periods. The tendon of the 2nd digit of 30 thoracic limbs of dogs was partially sectioned for glue application. Biopsies were performed 7, 15, and 30 days post surgery for the clinical and morphological study of tendons. Analysis of the results showed that 73.3% of the tendons showed stump retraction and 16.6% moderate to excessive adherence, which affected sliding. There was a significant difference in the number of inflammatory cells among the three studied periods, being the highest on day 15. The morphological analysis revealed a typical tendon healing process with a lower level of inflammation in the acute phase, facilitating the cicatricial maturation phase. Snake venom derived fibrin glue promotes the healing in dog flexor tendon.

  10. [Experience in fibrin glue application for the treatment of non-formed fistulas of the gastrointestinal tract].

    Science.gov (United States)

    Chernousov, A F; Khorobrykh, T V; Ishchenko, O V

    2006-01-01

    Fibrin glue was used for conservative closure of non-formed fistulas of the gastrointestinal tract in 38 patients. The variant of fibrin glue produced with cryoprecipitation (concentration of coagulant protein not less than 60 g/l) was applied as reparation stimulator. Fibrin glue has been used for closure of 8 duodenal and 4 gastric fistulas with chime loss to 1.5 l per day. Surgeries for palliative arrest of the intestinal loop with a fistula and classical obturators were not performed. The fistulas have been closed in 36 patients, 3 patients died (only 1 of them due to fistula). Fibrin glue reduced surgical aggression and improved treatment results in critically ill patients.

  11. Comparison of viability of adipose-derived Mesenchymal stem cells on agarose and fibrin glue scaffolds

    Directory of Open Access Journals (Sweden)

    Farzaneh Tafvizi

    2015-06-01

    Full Text Available Background & aim: Utilizing tissue engineering techniques and designing similar structures of the damaged tissues require the use of tools such as scaffolds, cells, and bioactive molecules in vitro. Meanwhile, appropriate cell cultures with the ability to divide and differentiate on the natural scaffolds lacking features like immunogenicity and tumorgenesis is particularly important. Adipose tissue has attracted researchers’ attention due to its abundance of mesenchymal stem cells and its availability through a liposuction. The purpose of the present study was to investigate the reproducibility and viability of the adipose-derived stem cells on natural scaffolds of fibrin glue and agarose. Methods: In the present experimental study, the isolation and identification of the mesenchymal stem cells was performed on tissue obtained from liposuction. The tissues were extensively washed with PBS and were digested with collagenase I, then the mesenchymal stem cells were isolated. The cells were cultured in RPMI medium supplemented with antibiotic. Subsequently, the expression of cell surface markers including CD34, CD44, CD90, and CD105 were analyzed by flow cytometry to confirm the mesenchymal cells. After preparing fibrin glue and agarose scaffolds, the viability and proliferation of the adipose tissue-derived mesenchymal stem cells were examined at the period of 24, 48, and 72 hours by MTT and ELISA assays. The obtained results were analyzed by SPSS ver.19. Results: The results of adipose tissue-derived mesenchymal stem cells culture on the fibrin glue and agarose scaffolds indicated that cell viability on fibrin glue and agarose scaffold were 68.22% and 89.75% in 24 hrs, 64.04% and 66.97% in 48 hours, 222.87% and 1089.68% in 72 hours respectively. Significant proliferation and viability cells on a synthesized agarose scaffold were seen compared to the fibrin glue scaffold after 72 hrs. The viability of the cells significantly increased on the

  12. Experimental closure of gunshot wounds by fibrin glue with antibiotics in pigs

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    Đenić Nebojša

    2015-01-01

    Full Text Available Background/Aim. Gunshot wounds caused by the automatic rifle M70AB2 (AK-47 7.62 mm, after the primary surgical management, were closed with delayed primary suture during the next four to seven days. This period coincides with the fibroblastic phase of wound healing. Fibrin glue is used as a local hemostatic and as a matrix for the local dosed release of antibiotics. Antibiotics addition to fibrin glue resulted in continuous diffusion into the surrounding next 4 to 7 days. The aim of this study was to create the preconditions for gunshot wounds closing without complications by the application of fibrin glue with antibiotics 24 h after primary surgical treatment. Methods. A total of 14 pigs were wounded in the gluteofemoral region by the bullet M67, initial velocity of 720 m/s. All wounded animals were surgically treated according to the principles of the warsurgery doctrine. Seven wounds were closed with primary delayed suture four days after the primary surgical treatment (traditional approach. Fibrin glue with antibiotics was introduced in seven wounds during the primary surgical treatment and primary delayed suture was done after 24 h. The macroscopic appearance and the clinical assessment of the wound were done during the primary surgical treatment and during its revision after 24 h, as well as histopathological findings at the days 4 and 7 after wounding. Results. Gunshot wounds caused by the automatic rifle M70AB2 (AK-47 7.62 mm, and treated with fibrin glue with antibiotics after primary surgical management, were closed with primary delayed suture after 24 h. In further wound evolution there were no complications. Conclusion. Uncomplicated soft-tissue wounds caused by an automatic M70AB2 rifle may be closed primarily with delayed suture without the risk of developing complications if on revision, 24 h after primary surgery, there were no present necrotic tissues, hematoma, and any signs of infection when fibrin glue with antibiotics

  13. Nitrogenous subcutaneous emphysema caused by spray application of fibrin glue during retroperitoneal laparoscopic surgery.

    Science.gov (United States)

    Matsuse, Shinji; Maruyama, Atsushi; Hara, Yoshiki

    2011-06-01

    We report a case of a patient treated by retroperitoneoscopic partial nephrectomy who developed nitrogenous subcutaneous emphysema (SCE) as a complication. The use of a nitrogen gas-pressured fibrin tissue adhesive applied as a spray caused excessively increased pressure in the closed retroperitoneal space and resulted in widespread SCE with protracted clinical course. To the best of our knowledge, this is the first report of nitrogenous SCE associated with pneumoperitoneum. The clinical significance of nitrogenous SCE is emphasized, and the risks associated with the use of fibrin glue as a spray during laparoscopic surgery are discussed.

  14. Application of collagen-chitosan/fibrin glue asymmetric scaffolds in skin tissue engineering*

    Science.gov (United States)

    Han, Chun-mao; Zhang, Li-ping; Sun, Jin-zhang; Shi, Hai-fei; Zhou, Jie; Gao, Chang-you

    2010-01-01

    To create a scaffold that is suitable for the construction of tissue-engineered skin, a novel asymmetric porous scaffold with different pore sizes on either side was prepared by combining a collagen-chitosan porous membrane with fibrin glue. Tissue-engineered skin was fabricated using this asymmetric scaffold, fibroblasts, and a human keratinocyte line (HaCaT). Epidermal cells could be seen growing easily and achieved confluence on the fibrin glue on the upper surface of the scaffold. Scanning electron microscopy showed typical shuttle-like fibroblasts adhering to the wall of the scaffold and fluorescence microscopy showed them growing in the dermal layer of the scaffold. The constructed composite skin substitute had a histological structure similar to that of normal skin tissue after three weeks of culture. The results of our study suggest that the asymmetric scaffold is a promising biologically functional material for skin tissue engineering, with prospects for clinical applications. PMID:20593518

  15. Application of collagen-chitosan/fibrin glue asymmetric scaffolds in skin tissue engineering.

    Science.gov (United States)

    Han, Chun-mao; Zhang, Li-ping; Sun, Jin-zhang; Shi, Hai-fei; Zhou, Jie; Gao, Chang-you

    2010-07-01

    To create a scaffold that is suitable for the construction of tissue-engineered skin, a novel asymmetric porous scaffold with different pore sizes on either side was prepared by combining a collagen-chitosan porous membrane with fibrin glue. Tissue-engineered skin was fabricated using this asymmetric scaffold, fibroblasts, and a human keratinocyte line (HaCaT). Epidermal cells could be seen growing easily and achieved confluence on the fibrin glue on the upper surface of the scaffold. Scanning electron microscopy showed typical shuttle-like fibroblasts adhering to the wall of the scaffold and fluorescence microscopy showed them growing in the dermal layer of the scaffold. The constructed composite skin substitute had a histological structure similar to that of normal skin tissue after three weeks of culture. The results of our study suggest that the asymmetric scaffold is a promising biologically functional material for skin tissue engineering, with prospects for clinical applications.

  16. Biocompatibility, biodegradation, and neovascularization of human single-unit platelet-rich fibrin glue: an in vivo analysis

    Institute of Scientific and Technical Information of China (English)

    Wu Xiuwen; Ren Jianan; Yao Genhong; Zhou Bo; Wang Gefei; Gu Guosheng; Luan Jianfeng

    2014-01-01

    Background The clinical applications of fibrin glue span over several surgical modalities.The aim of this study was to evaluate the biocompatibility and biodegradation of different formulations of platelet-rich fibrin glue in vivo and examine its effects on the neovascularization of wound sites.Methods Human-derived single-unit fibrin glue was prepared.Incisions were made on the backs of rats,and these were coated with homemade glues containing different concentrations of aminomethylbenzoic acid (Groups A-F) or commercial adhesives (Group G).A sham control group was included (Group H).The wounds were examined by histological analysis and immunohistochemistry at several time points.Results Successful wound closure was achieved in all groups by day 12.Acute inflammation occurred during the first six days,but gradually disappeared.The longest sealant duration was achieved using the lowest concentration of antifibrinolytic agent in a 1:10 volume ratio with cryoprecipitate.Expression levels of the platelet endothelial cell adhesion molecule-1 were significantly higher in Groups A and C compared to the control groups (Groups G and H) on day 3 (P <0.05).Conclusions Single-unit platelet-rich fibrin glue has excellent biocompatibility and is associated with the upregulation of neovascularization.The addition of aminomethylbenzoic acid could prevent the degradation of fibrin glue.

  17. Fibrin Glue Does Not Improve Healing of Gastrointestinal Anastomoses

    DEFF Research Database (Denmark)

    Nordentoft, Tyge; Pommergaard, Hans-Christian; Rosenberg, Jacob

    2015-01-01

    BACKGROUND/AIM: Anastomotic leakage remains a frequent and serious complication in gastrointestinal surgery. In order to reduce its incidence, several clinical and experimental studies on anastomotic sealing have been performed. In a number of these studies, the sealing material has been fibrin...... on the healing effects of FG on gastrointestinal anastomoses. METHODS: PubMed, EMBASE and the Cochrane databases were searched for studies evaluating the healing process of gastrointestinal anastomoses after any kind of FG application. The search period was from 1953 to December 2013. RESULTS: Twenty...

  18. Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses

    Directory of Open Access Journals (Sweden)

    Eliseo Portilla-de Buen

    2014-04-01

    Full Text Available OBJECTIVE: Fibrin glues have not been consistently successful in preventing the dehiscence of high-risk colonic anastomoses. Fibrinogen and thrombin concentrations in glues determine their ability to function as sealants, healers, and/or adhesives. The objective of the current study was to compare the effects of different concentrations of fibrinogen and thrombin on bursting pressure, leaks, dehiscence, and morphology of high-risk ischemic colonic anastomoses using fibrin glue in rats. METHODS: Colonic anastomoses in adult female Sprague-Dawley rats (weight, 250-350 g treated with fibrin glue containing different concentrations of fibrinogen and thrombin were evaluated at post-operative day 5. The interventions were low-risk (normal or high-risk (ischemic end-to-end colonic anastomoses using polypropylene sutures and topical application of fibrinogen at high (120 mg/mL or low (40 mg/mL concentrations and thrombin at high (1000 IU/mL or low (500 IU/mL concentrations. RESULTS: Ischemia alone, anastomosis alone, or both together reduced the bursting pressure. Glues containing a low fibrinogen concentration improved this parameter in all cases. High thrombin in combination with low fibrinogen also improved adherence exclusively in low-risk anastomoses. No differences were detected with respect to macroscopic parameters, histopathology, or hydroxyproline content at 5 days post-anastomosis. CONCLUSIONS: Fibrin glue with a low fibrinogen content normalizes the bursting pressure of high-risk ischemic left-colon anastomoses in rats at day 5 after surgery.

  19. EFFICACY OF THROMBIN FIBRIN GLUE AND SCLE ROSANT IN THE MANAGEMENT OF BLEEDI NG GASTRIC VARICES

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    Sanjay Gupta

    2015-01-01

    Full Text Available Gastric varices are noted in up to 20 % of patents with portal hypertension , and are more common in those with non - cirrhotic etiology 1 . They bleed at lower portal pressures , bleed more severely and are associated with higher rates of rebleed , encephalopathy and mortality 1,2,3 . Variceal obliteration using tissue adhesives such as N - butyl cyanoacrylate leading to plugging and thrombosis of the gastric varices is currently the first line management option for obliteration of the gastric varices 3 . Although various options have been proposed , gold standard for management of gastric variceal bleeds is yet to be defined. We theorized that injection of the gastric varices using thrombin based glue followed by injection of a sclerosant shall be effective in optimum sclerotherapy and eradication of gastric varices. MATERIAL AND METHODS : All patients presenting with gastric variceal bleed were offered sclerotherapy with Thrombin fibrin based glue and sclerosant (TFG/S . During the study period 18 patients were enrolled in the TGF/S group. 21 patients underwent variceal plugging with n - butyl cyanoacrylate (NBC . There was no significant difference in age/ sex , duration of bleed or time interval between onset of bleed and endotherapy. RESULTS: Patients undergoing endotherapy with TGF/S had less episodes of bleed , and greater eradication of varices. CONCLUSION: The results with thrombin / fibrin glue and sclerotherapy are highly encouraging. Well - designed trials need to be performed KEYWORDS:Gastric varices; Thrombin Sclerotherapy

  20. Fibrin glue repair leads to enhanced axonal elongation during early peripheral nerve regeneration in an in vivo mouse model

    Institute of Scientific and Technical Information of China (English)

    Georgios Koulaxouzidis; Gernot Reim; Christian Witzel

    2015-01-01

    Microsurgical suturing is the gold standard of nerve coaptation. Although literature on the usefulness of ifbrin glue as an alternative is becoming increasingly available, it remains contradic-tory. Furthermore, no data exist on how both repair methods might inlfuence the morphological aspects (arborization; branching) of early peripheral nerve regeneration. We used the sciatic nerve transplantation model in thy-1 yellow lfuorescent protein mice (YFP;n = 10). Pieces of nerve (1cm) were grafted from YFP-negative mice (n = 10) into those expressing YFP. We per-formed microsuture coaptations on one side and used ifbrin glue for repair on the contralateral side. Seven days after grafting, the regeneration distance, the percentage of regenerating and ar-borizing axons, the number of branches per axon, the coaptation failure rate, the gap size at the repair site and the time needed for surgical repair were all investigated. Fibrin glue repair resulted in regenerating axons travelling further into the distal nerve. It also increased the percentage of arborizing axons. No coaptation failure was detected. Gap sizes were comparable in both groups. Fibrin glue signiifcantly reduced surgical repair time. The increase in regeneration distance, even after the short period of time, is in line with the results of others that showed faster axonal regen-eration after ifbrin glue repair. The increase in arborizing axons could be another explanation for better functional and electrophysiological results after ifbrin glue repair. Fibrin glue nerve coap-tation seems to be a promising alternative to microsuture repair.

  1. Platelet-Rich Fibrin: An Autologous Fibrin Matrix in Surgical Procedures: A Case Report and Review of Literature

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    Amir Hossein Nejat

    2011-01-01

    Full Text Available Introduction: The healing process after surgery is a challenging issue for surgeons. Various materials and techniques have been developed to facilitate this process and reduce its period. Fibrin adhesives are often used in cardiothoracic and vascular surgery to seal diffuse microvascular bleeding and in general and plastic surgery to seal wound borders. This Case report and literature review will introduce the various usages of platelet-rich fibrin in different surgical procedures and the method of producing the matrix. Case Report: A 24-year old man with periorbital skin avulsion treated with PRF membrane has been reported and discussed in this paper.  Conclusion: Platelet-rich fibrin is a natural autologous fibrin matrix, which can be produced with a simple blood sample and a table centrifuge. The material has been used in a wide range of surgical procedures to shorten the healing period and reduce post-surgical complications.

  2. Local Application of BMP-2 Specific Plasmids in Fibrin Glue does not Promote Implant Fixation

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    Plank Christan

    2011-07-01

    Full Text Available Abstract Background BMP-2 is known to accelerate fracture healing and might also enhance osseointegration and implant fixation. Application of recombinant BMP-2 has a time-limited effect. Therefore, a gene transfer approach with a steady production of BMP-2 appears to be attractive. The aim of this study was to examine the effect of locally applied BMP-2 plasmids on the bone-implant integration in a non-weight bearing rabbit tibia model using a comparatively new non-viral copolymer-protected gene vector (COPROG. Methods Sixty rabbits were divided into 4 groups. All of them received nailing of both tibiae. The verum group had the nails inserted with the COPROG vector and BMP-2 plasmids using fibrin glue as a carrier. Controls were a group with fibrin glue only and a blank group. After 28 and 56 days, these three groups were sacrificed and one tibia was randomly chosen for biomechanical testing, while the other tibia underwent histomorphometrical examination. In a fourth group, a reporter-gene was incorporated in the fibrin glue instead of the BMP-2 formula to prove that transfection was successful. Results Implant fixation strength was significantly lower after 28 and 56 days in the verum group. Histomorphometry supported the findings after 28 days, showing less bone-implant contact. In the fourth group, successful transfection could be confirmed by detection of the reporter-gene in 20 of 22 tibiae. But, also systemic reporter-gene expression was found in heterotopic locations, showing an undesired spreading of the locally applied gene formula. Conclusion Our results underline the transfecting capability of this vector and support the idea that BMP-2 might diminish osseointegration. Further studies are necessary to specify the exact mechanisms and the systemic effects.

  3. Successful application of subcutaneous adipose tissue with fibrin glue in conservative treatment of tracheobronchial rupture.

    Science.gov (United States)

    Tokuishi, Keita; Yamamoto, Satoshi; Anami, Kentaro; Moroga, Toshihiko; Miyawaki, Michiyo; Chujo, Masao; Yamashita, Shin-Ichi; Kawahara, Katsunobu

    2012-11-01

    An 84-year-old woman underwent aortic and mitral valve replacement. After weaning from cardiopulmonary bypass, hemorrhage was observed in the endobronchial tube. The bleeding bronchus was isolated to protect the airway using the blocker cuff of a Univent tube (Fuji Systems Corp, Tokyo, Japan). Computed tomography showed a pulmonary pseudoaneurysm in the left upper lobe. She underwent selective pulmonary angiography and embolization of the pseudoaneurysm. Bronchoscopy revealed a 5-mm bronchial rupture at the left upper lobe bronchus. The laceration was filled with adipose tissue and fibrin glue. Bronchoscopy showed a completely reepithelialized membrane, and she was discharged 38 days postoperatively.

  4. Effect of intraoperative platelet-rich plasma and fibrin glue application on skin flap survival.

    Science.gov (United States)

    Findikcioglu, Fulya; Findikcioglu, Kemal; Yavuzer, Reha; Lortlar, Nese; Atabay, Kenan

    2012-09-01

    The experiment was designed to compare the effect of intraoperative platelet-rich plasma (PRP) and fibrin glue application on skin flap survival. In this study, bilateral epigastric flaps were elevated in 24 rats. The right-side flaps were used as the control of the left-side flaps. Platelet-rich plasma, fibrin glue, and thrombin had been applied under the flap sites in groups 1, 2, and 3, respectively. Five days later, all flap pedicles were ligated. Necrotic area measurements, microangiography, and histologic and immunohistochemical evaluations were performed to compare the groups. Platelet-rich plasma reduced necrotic area percentages as compared with other groups. Histologically and microangiographically increased number of arterioles were observed in PRP groups. Thrombin when used alone increased flap necrosis. Vascular endothelial growth factor, platelet-derived growth factor, and transforming growth factor β3 primary antibody staining showed increased neovascularization and reepithelialization in all PRP-applied flaps. This study demonstrated that PRP, when applied intraoperatively under the skin flap, may enhance flap survival. Thrombin used alone was found to be unsuitable in flap surgery.

  5. Transplantation of adipose-derived stem cells with fibrin glue for treatment of acute myocardial infarction in rat

    Institute of Scientific and Technical Information of China (English)

    张雪莲

    2013-01-01

    Objective To investigate the cell survival of the combination of fibrin glue and adiposederived stem cells(ADSCs) in rats when implanted into ischemic myocardium and the improvement of heart function. Methods The rat ADSCs were isolated from the subcutaneous adipose

  6. Management of urine leak after laparoscopic cyst decortication with retrograde endoscopic fibrin glue application and ureteral stent placement.

    Science.gov (United States)

    Chen, Mang L; Tomaszewski, Jeffrey J; Matoka, Derek J; Ost, Michael C

    2011-01-01

    Urine leakage is an uncommon complication after renal cyst decortication that typically resolves with adequate drainage. With prolonged large volume urine leakage from a perinephric drain, however, consideration for open surgical repair must be taken into account. We present the successful management of persistent urine leakage after laparoscopic cyst decortication with endoscopic retrograde fibrin glue injection and ureteral stent placement.

  7. Reduction of bone resorption by the application of fibrin glue in the reconstruction of the alveolar cleft.

    Science.gov (United States)

    Segura-Castillo, José L; Aguirre-Camacho, Humberto; González-Ojeda, Alejandro; Michel-Perez, Jorge

    2005-01-01

    A major complication in 30% to 75% of cases of surgical treatment of alveolar cleft is resorption of the bone graft. A treatment alternative is the application of fibrin glue, which has the capacity to favor the integration of the graft. The main objective of the study was to evaluate if the use of the fibrin glue reduces bone resorption when it is applied locally. The authors designed a randomized clinical trial. Patients were divided into two groups: group 1, fibrin glue; and group 2, control. Pre- and postoperative graft volume, bone density, bone quality (Lekholm and Zarb, and Norton and Gamble classifications), and postoperative complications were evaluated. The follow-up for all patients was 3 months after discharge. Twenty-seven patients were surgically treated, 13 in group 1 and 14 in group 2. Group 1 had increased graft volume compared with group 2 (64.32 cm v 21.70 cm; P 0.076). Bone quality was type 1, 2 and 3 and 4 in group 1. Resorption in group 2 was 62.26%; in group 1, it was 29.72% (P > 0.081). The observed complications were infection and dehiscence of sutures (P > 0.537). The authors conclude that the fibrin glue significantly diminishes bone resorption, allowing improved graft integration and quality.

  8. Tulip bundle technique and fibrin glue injection:Unusual treatment of colonic perforation

    Institute of Scientific and Technical Information of China (English)

    Filippo Mocciaro; Gabriele Curcio; Ilaria Tarantino; Luca Barresi; Marco Spada; Sergio Li Petri; Mario Traina

    2011-01-01

    We report a case of a 63-year-old male who experienced an iatrogenic sigmoid perforation repaired combining three endoscopic techniques.The lesion was large and irregular with three discrete perforations, therefore,we decided to close it by placing one clip per perforation,and then connecting all the clips with two endoloops.Finally we chose to use a fibrin glue injection to obtain a complete sealing.Four days after the colonoscopy the patient underwent a laparoscopic right hemicolectomy due to evidence of a large polyp of the caecum with high grade dysplasia and focal carcinoma in situ .Inspection of the sigma showed complete repair of the perforation.This report underlines how a conservative approach,together with a combination of various endoscopic techniques,can resolve complicated iatrogenic perforations of the colon.

  9. A New Potential Cause in the Development of Toxic Anterior Segment Syndrome: Fibrin Glue

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    Selçuk Sızmaz

    2014-08-01

    Full Text Available Objectives: To present a potential cause for toxic anterior segment syndrome (TASS. Materials and Methods: We report 4 cases of TASS that occurred following uneventful phacoemulsification and intraocular lens implantation. Results: The 4 cases were the first consecutive 2 cases of 2 different surgery days, 5 months apart. The most prominent sign of TASS was limbus-to-limbus corneal edema. Pain and/or intraocular pressure rise were also common. All surgical and presurgical procedures were checked after the first outbreak, whereas the second outbreak required further investigation. Fibrin glue remnants from preceding pterygium surgery with conjunctival autografting were found to be the potential cause. Despite intensive corticosteroid therapy, corneal edema did not resolve in 2 patients who underwent keratoplasty. Conclusion: TASS is a sight-threatening condition which requires thorough investigation for prevention of new cases. All steps must be carefully revised. (Turk J Ophthalmol 2014; 44: 280-3

  10. The Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Their Conditioned Media Topically Delivered in Fibrin Glue on Chronic Wound Healing in Rats

    OpenAIRE

    Mehanna, Radwa A.; Iman Nabil; Noha Attia; Bary, Amany A.; Razek, Khalid A.; Ahmed, Tamer A. E.; Fatma Elsayed

    2015-01-01

    Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent a modern approach for management of chronic skin injuries. In this work, we describe BM-MSCs application versus their conditioned media (CM) when delivered topically admixed with fibrin glue to enhance the healing of chronic excisional wounds in rats. Fifty-two adult male rats were classified into four groups after induction of large-sized full-thickness skin wound: control group (CG), fibrin only group (FG), fibrin + MSCs group (...

  11. Differentiation within autologous fibrin scaffolds of porcine dermal cells with the mesenchymal stem cell phenotype

    Energy Technology Data Exchange (ETDEWEB)

    Puente, Pilar de la, E-mail: pilardelapuentegarcia@gmail.com [Tissue Bank, San Francisco Clinic Foundation, Av./Facultad 51, 5°, 24004 León (Spain); Ludeña, Dolores [Pathology Service, University Hospital of Salamanca, P/San Vicente 58-182, 37007 Salamanca (Spain); López, Marta; Ramos, Jennifer; Iglesias, Javier [Tissue Bank, San Francisco Clinic Foundation, Av./Facultad 51, 5°, 24004 León (Spain)

    2013-02-01

    Porcine mesenchymal stem cells (pMSCs) are an attractive source of cells for tissue engineering because their properties are similar to those of human stem cells. pMSCs can be found in different tissues but their dermal origin has not been studied in depth. Additionally, MSCs differentiation in monolayer cultures requires subcultured cells, and these cells are at risk of dedifferentiation when implanting them into living tissue. Following this, we attempted to characterize the MSCs phenotype of porcine dermal cells and to evaluate their cellular proliferation and differentiation in autologous fibrin scaffolds (AFSs). Dermal biopsies and blood samples were obtained from 12 pigs. Dermal cells were characterized by flow cytometry. Frozen autologous plasma was used to prepare AFSs. pMSC differentiation was studied in standard structures (monolayers and pellets) and in AFSs. The pMSCs expressed the CD90 and CD29 markers of the mesenchymal lineage. AFSs afforded adipogenic, osteogenic and chondrogenic differentiation. The porcine dermis can be proposed to be a good source of MSCs with adequate proliferative capacity and a suitable expression of markers. The pMSCs also showed optimal proliferation and differentiation in AFSs, such that these might serve as a promising autologous and implantable material for use in tissue engineering. -- Highlights: ► Low fibrinogen concentration provides a suitable matrix for cell migration and differentiation. ► Autologous fibrin scaffolds is a promising technique in tissue engineering. ► Dermal cells are an easily accessible mesenchymal stem cell source. ► Fibrin scaffolds afforded adipogenic, osteogenic and chondrogenic differentiation.

  12. "Hemosuccus pancreaticus"--primarily ultrasound-guided successful intervention using transcutaneous fibrin glue application and histoacryl injection.

    Science.gov (United States)

    Will, U; Mueller, A-K; Grote, R; Meyer, F

    2008-12-01

    There is a broad spectrum of causes for upper gastrointestinal (GI) bleeding that can be stopped by various approaches. On the basis of the report of an extraordinary case, the favorable minimally invasive approach of applying fibrin glue and histoacryl/lipiodol to the vascular basis of a bleeding pseudoaneurysm leading to "Hemosuccus pancreaticus" as a rare cause of recurrent bleeding in the upper GI tract and dangerous complications in the case of chronic pancreatitis is described. There were recurrent bleeding episodes within the upper GI tract in a 40-year-old female patient. Her medical history was significant for chronic pancreatitis and pseudocyst. Abdominal ultrasound plus duplex ultrasonography revealed a pseudoaneurysm within the tail of the pancreas as the cause of "Hemosuccus pancreaticus". Ultrasound guidance was used to repeatedly apply 2 ml of fibrin glue and 2 x 2 ml of the mixture of lipiodol and histoacryl to the basis of the pseudoaneurysm which led to complete and permanent cessation of the bleeding. Immediate and follow-up control duplex ultrasonographies (up to one year) demonstrated sufficient exclusion of the pseudoaneurysm but a preservation of the lienal artery with no disturbance of the blood perfusion in the splenic parenchyma. In conclusion, this is one of the first reports of the successful cessation of recurrent bleeding into a pseudocyst out of pseudoaneurysm ("Hemosuccus pancreaticus") by an ultrasound-guided transcutaneous fibrin glue and histoacryl/lipiodol application, which 1. is recommended as an alternative but feasible and safe therapeutic tool, 2. can provide sufficient and permanent cessation of bleeding but preserve the perfusion of the natural vessel as an initial step in the possible therapeutic algorithm, and 3. can avoid, in case of success, more invasive approaches such as angiography-guided embolization with coils or implantation of a prosthesis and even open surgical intervention, in particular, in high

  13. 生物蛋白胶在PPH手术中的应用%Applications of fibrin glue in PPH surgery

    Institute of Scientific and Technical Information of China (English)

    耿鹏; 牛跃平; 任潇毅; 杜绍先

    2011-01-01

    Objective To evaluate the curative effect of fibrin glue on postoperative bleeding after procedure for prolappsed hemorrhoids. Methods Totally 84 patients, reciveing procedure for prolap-psed hemorrhoids, underwent radical surgery from March 2006 to March 2009 were randomly divided into two groups. The control group began the routine hemostatic, local oil gauze oppression; The experimental group were applied with fibrin glue on anastomoses. On the first day after surgery, the ooze blood and ooze blood duration were observed. Results After the first day ooze blood and ooze blood duration of the experimental group were significantly lower than that of the control group (P < 0. 01). Conclusions Fibrin glue can reduce the postoperative bleeding after procedure for prolappsed hemorrhoids, and did not find adverse reactions.%目的 评价生物蛋白胶在痔上粘膜环切手术(PPH)中减少术后出血的疗效.方法 对2006年3月-2010年3月行PPH手术患者84例随机分成两组,对照组术后常规止血,局部用油纱布压迫;实验组在常规处理的基础上在吻合口处喷洒生物蛋白胶封闭.观察术后第一天渗血量及渗血持续时问.结果 应用生物蛋白胶组术后第一天渗血量及渗血持续时间均明显低于对照组(P<0.01).结论 生物蛋白胶在PPH手术中应用可减少术后出血,未发现不良反应.

  14. Management of posteriorly dislocated crystalline lens with perfluorocarbon liquid and fibrin glue-assisted scleral-fixated intraocular lens implantation.

    Science.gov (United States)

    Lee, Soo Jung; Kim, In Geun; Park, Jung Min

    2013-03-01

    We describe a technique that uses a 23-gauge transconjunctival sutureless vitrectomy with perfluorocarbon liquid (PFCL) and phacoemulsification to manage a dropped nucleus. The PFCL is injected into the vitreous space until the dislocated lens reaches the iris plane and is then removed using phacoemulsification in the anterior chamber. After intraocular lens (IOL) implantation, a 23-gauge forceps is passed through the sclerotomy to grasp the IOL haptic, which is pulled onto the ocular surface. Tunnels are made at the edge of the flap with a 26-gauge needle into which the 2 haptics are tucked for additional stability. The scleral flaps and conjunctiva are then glued using biological glue. Perfluorocarbon liquid reduces lens repulsion and blocks the transmission of the ultrasound stream to the retina. The fibrin glue-assisted sutureless IOL implantation technique could reduce complications and suture-related problems.

  15. Effect of thrombin concentration on the adhesion strength and clinical application of fibrin glue-soaked sponge.

    Science.gov (United States)

    Campos, Francia; Fujio, Shingo; Sugata, Sei; Tokimura, Hiroshi; Hanaya, Ryosuke; Bohara, Manoj; Arita, Kazunori

    2013-01-01

    Fibrin glue-soaked gelatin sponge (FGGS) has been used for tissue sealing in neurosurgical practice, but too rapid clotting of fibrin glue occasionally prevents good fixation of FGGS. Dilution of thrombin may provide adequate manipulation time between mixing fibrinogen and thrombin on gelatin sponge and application into the tissue defects. The present study characterized the effect of thrombin dilution on the adhesion strength of FGGS and retrospectively assessed the clinical usage of the dilution for filling dead space or sealing arachnoid defect in 255 cases who underwent transsphenoidal surgery for the last 66 months. FGGS was prepared using three different concentrations of thrombin: 250 (standard), 50 (1:5 dilution), and 25 (1:10 dilution) units/ml, and incubated for three different periods (5, 20, and 60 seconds). FGGSs were applied over two adjacently positioned porcine skins placed on two metallic plates. The adhesion strength was evaluated by measuring maximum tensile strength during pulling out the sliding plate at a constant rate of displacement. The maximum adhesion strength was greater for FGGS with 1:10 diluted thrombin solution than for FGGS prepared with higher concentrations (p < 0.05). Adhesion strength did not decay for 20 seconds after the mixture. Only four of 255 cases (1.6%) required second reconstruction of sella floor due to the cerebrospinal fluid leakage. FGGS prepared with diluted thrombin solution can provide adequate adhesion strength for clinical use.

  16. Management of an extrasphincteric fistula in an HIV-positive patient by using fibrin glue: a case report with tips and tricks

    Directory of Open Access Journals (Sweden)

    Sapalidis Konstantinos

    2010-02-01

    Full Text Available Abstract Background Individuals with impaired immunity are at higher risk of perianal diseases. Concerning complex anal fistulas impaired healing and complication rates are also higher. Definitive treatment of a fistula aims controlling the purulent discharge and prevents its recurrence. It depends mainly on the trajectory of the fistula and the underlying disease. We present a case of a HIV-positive patient with a complex extrasphincteric anal fistula who was treated successfully with fibrin glue application. We further, discuss tips and tricks when applying fibrin glue as plugging material in complex anal fistulas. Case presentation A sixty-one-year-old HIV-positive male referred to us for warts and extrasphincteric fistula. Because of the patients' immunological status, we opted against surgery and recommended fibrin glue plugging. The patient was discharged the same day. A follow-up examination was performed 5 days after the initial fibrin glue application showing that the fistula canal was obstructed. Three months and a year post-intervention the fistula tract remains closed. Conclusion The best treatment for a disease gives at least the same result with the other treatments with minimised risk for the life of the patient and minimal application effort. Conservative closure of fistula with fibrin plugging is simple, safe and with less morbidity than surgery. Our patient was successfully treated without endangering his life despite his precarious medical state. Not everybody believes in the effectiveness of fibrin glue application, however we consider this solution in cases of complex fistulas at least as primary procedure in special populations such as the immunosupressed.

  17. Use of fibrin glue derived from snake venom in the repair of deep corneal ulcers: experimental study in dogs (Canis familiaris, Linnaeus, 1758

    Directory of Open Access Journals (Sweden)

    R. L. Sampaio

    2007-01-01

    Full Text Available Fibrin glue has been researched as an alternative method for tissue synthesis and is known for its capability to promote hemostasis at the application site, good approximation of wound edges and fast healing. The current study consisted in the application of fibrin glue derived from snake venom as treatment for experimental corneal ulcers. Twenty-one dogs had their corneas experimentally prepared through lamellar keratectomy (of standardized diameter and depth. Animals were divided into seven groups of three animals each. Six experimental groups were periodically evaluated and collection was carried out on the 1st, 3rd, 7th, 15th, 30th and 60th post-operative days, whereas one control group was evaluated throughout the experiment. Analyses consisted in the clinical evolution and in the histopathological study of all operated on eyes. Results indicated that fibrin glue was efficient in repairing keratectomy wounds in dogs and contributed to an earlier healing phenomenon, avoiding edema formation and keeping corneal clearness. The use of fibrin glue derived from snake venom showed to be easy to apply, feasible with animal models and of low cost, avoiding the lesion progress and allowing fast and appropriate corneal healing.

  18. Haemostasis with fibrin glue injection into the pericardial space for right ventricular perforation caused by an iatrogenic procedural complication.

    Science.gov (United States)

    Arai, Hirofumi; Miyamoto, Takamichi; Hara, Nobuhiro; Obayashi, Tohru

    2016-05-17

    An 89-year-old woman with severe aortic valve stenosis and bradycardia presented with circulatory shock due to cardiac tamponade. We performed pericardiocentesis, and then diagnosed right ventricular perforation by echocardiography with microcavitation contrast medium just before inserting a drainage tube. We then inserted the drainage tube in the appropriate position and withdrew blood-filled fluid. The patient was haemodynamically stabilised, but haemorrhage from the perforation site continued for a few days. We injected fibrin glue into the pericardial space through the drainage tube and achieved haemostasis. Thus, we avoided surgery to close the perforation in this high-risk patient. There was no recurrence of haemorrhage. She subsequently had elective aortic valve replacement at another hospital. No adhesions in the pericardial space were seen during surgery.

  19. Premature rupture of membranes at 20 weeks: report of a successful outcome after transcervical application of fibrin glue.

    Science.gov (United States)

    Calado, Elsa; Ayres-de-Campos, Diogo

    2007-01-01

    A 30-year-old primigravida was admitted to hospital at 20 weeks of gestation because of premature rupture of membranes and oligohydramnios. The patient was maintained in bed rest and given intravenous ampicillin. Forty-eight hours later, after documenting the absence of infection and maintenance of the oligohydramnios, fibrin glue was applied transcervically under ultrasound control. There was subjective improvement in amniotic fluid volume after treatment, but always within the criteria of oligohydramnios. Fibrin glue application was repeated twice due to reported increase in fluid loss and diminished amniotic fluid volume on ultrasound. Amoxicillin per os was started at 23 weeks, and clavulanic acid was added at 26 weeks due to the isolation of an Escherichia coli on cervical-vaginal cultures. No signs of infection ensued until 34 weeks, when an axillary temperature of 39.5 degrees C was detected together with a non-reassuring cardiotocographic pattern, the latter leading to the performance of an urgent cesarean section. The newborn had an Apgar score of 9/10/10, umbilical artery pH of 7.32, and no external deformities. He showed no signs of lung hypoplasia and required no oxygen supplementation. Oropharyngeal and blood cultures revealed an E. coli infection and antibiotic treatment was started. No further complications occurred and he was discharged home on the 8th day of life. At 12 months, the child reveals a normal development. The mother had a mild and short-lasting wound infection and was discharged on the 8th postoperative day.

  20. Fibrin glue is a candidate scaffold for long-term therapeutic protein expression in spontaneously differentiated adipocytes in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Aoyagi, Yasuyuki [Center for Advanced Medicine, Chiba University Hospital, Chiba University, Chiba (Japan); Department of Genome Research and Clinical Application, Graduate School of Medicine, Chiba University, Chiba (Japan); Kuroda, Masayuki, E-mail: kurodam@faculty.chiba-u.jp [Center for Advanced Medicine, Chiba University Hospital, Chiba University, Chiba (Japan); Department of Genome Research and Clinical Application, Graduate School of Medicine, Chiba University, Chiba (Japan); Asada, Sakiyo [Center for Advanced Medicine, Chiba University Hospital, Chiba University, Chiba (Japan); Department of Genome Research and Clinical Application, Graduate School of Medicine, Chiba University, Chiba (Japan); Tanaka, Shigeaki; Konno, Shunichi; Tanio, Masami; Aso, Masayuki [CellGenTech, Inc., Chiba (Japan); Okamoto, Yoshitaka [Center for Advanced Medicine, Chiba University Hospital, Chiba University, Chiba (Japan); Nakayama, Toshinori [Department of Immunology, Graduate School of Medicine, Chiba University, Chiba (Japan); Saito, Yasushi [Chiba University, Chiba (Japan); Bujo, Hideaki [Department of Genome Research and Clinical Application, Graduate School of Medicine, Chiba University, Chiba (Japan)

    2012-01-01

    Adipose tissue is expected to provide a source of cells for protein replacement therapies via auto-transplantation. However, the conditioning of the environment surrounding the transplanted adipocytes for their long-term survival and protein secretion properties has not been established. We have recently developed a preparation procedure for preadipocytes, ceiling culture-derived proliferative adipocytes (ccdPAs), as a therapeutic gene vehicle suitable for stable gene product secretion. We herein report the results of our evaluation of using fibrin glue as a scaffold for the transplanted ccdPAs for the expression of a transduced gene in a three-dimensional culture system. The ccdPAs secreted the functional protein translated from an exogenously transduced gene, as well as physiological adipocyte proteins, and the long viability of ccdPAs (up to 84 days) was dependent on the fibrinogen concentrations. The ccdPAs spontaneously accumulated lipid droplets, and their expression levels of the transduced exogenous gene with its product were maintained for at least 56 days. The fibrinogen concentration modified the adipogenic differentiation of ccdPAs and their exogenous gene expression levels, and the levels of exogenously transduced gene expression at the different fibrinogen concentrations were dependent on the extent of adipogenic differentiation in the gel. These results indicate that fibrin glue helps to maintain the high adipogenic potential of cultured adipocytes after passaging in a 3D culture system, and suggests that once they are successfully implanted at the transplantation site, the cells exhibit increased expression of the transduced gene with adipogenic differentiation.

  1. Characterization of the biological effect of fish fibrin glue in experiments on rats: Immunological and coagulation studies

    Science.gov (United States)

    Laidmäe, Ivo; Salum, Tiit; Sawyer, Evelyn S.; Janmey, Paul A.; Uibo, Raivo

    2011-01-01

    Fibrin glues (FG) of human or bovine origin are widely used for haemostasis and wound healing. In addition FGs are studied in many biomedical areas like cell therapy or tissue engineering. As any mammalian plasma products FG-s pose risk of transmission of bacteria, viruses, or prions and may compromise patient homeostasis. In this study, we examined coagulation parameters and immunological status of rats treated with salmon-derived FG. We evaluated the changes in thrombin time, prothrombin activity, and presence of antibodies on 46 Wistar rats. This study shows that salmon-derived FG, injected intraperitoneally, does not cause coagulation disturbances in the peripheral blood. After a first challenge with salmon-derived FG there were low but detectable amounts of antibodies revealed by ELISA and immunoblot. After a second administration there was substantial elevation of antibodies to FG components and other copurifying plasma proteins. Antibody reactivity to human Factor Va, revealed in three animals, was not associated with FG application. Taken together, blood immunological and coagulation parameters support the suitability of salmon-derived FG in the development of fibrin sealants for medical use. PMID:19484773

  2. Effective approach for the treatment of bronchopleural fistula: application of endovascular metallic ring-shaped coil in combination with fibrin glue.

    Science.gov (United States)

    Sivrikoz, Cumhur M; Kaya, Tamer; Tulay, Cumhur M; Ak, Ilknur; Bilir, Ayten; Döner, Egemen

    2007-06-01

    The development of bronchopleural fistula is an important complication after pulmonary resections. Generally, conventional treatment methods are used in patients having bronchopleural fistulas. Recently, there has been an increase in the use of minimally invasive methods yielding better results. In our study, we applied a combination of endovascular metallic ring coil and fibrin glue. We hereby think that such an approach for a combination might be a contribution to improving the already existing minimally invasive treatment methods.

  3. Does Fibrin Glue Sealant Used During Pancreaticoduodenectomy Reduce Post-Operative Complication? Audit of 100 Consecutive Patients Undergoing Whipple's Procedure by a Single Surgeon

    Directory of Open Access Journals (Sweden)

    Shafiq Rehman

    2016-03-01

    Full Text Available Introduction This study evaluated the effect of fibrin glue applied as a sandwich film between a two layer pancreatico-jejunostomy anastomosis following pancreaticoduodenectomy. Primary end-points were post-operative pancreatic fistulae, overall complication rates and post-operative length of stay. Methods Pancreaticoduodenectomy was performed by fashioning a two layer pancreatico-jejunostomy with or without a glue sealant which when applied, formed a thin film external to the ductal anastomosis but internal to the seromuscularpancreatic parenchymal layer. Results Following audit of 100 consecutive patients undergoing pancreaticoduodenectomy, patients were separated into two groups; those with glue augmented anastomosis [Glue (G N=50] or those without [No Glue (NG, N=50]. Each group was matched with regard to age [median, G=68 years vs. NG=66 years, (P=0.19] and sex (P=0.84. There were no statistically significant differences between the two groups with respect to overall POPF [G N=7(14% vs. NG N=11(22%, (P=0.42], significant complications (Clavien Grade 3 or more, [G N=4(8% vs. NG N=2(4% (P=0.40], or post-operative length of stay [G 13 days vs. NG 14 days, (P=0.90]. In those patients with the highest fistulae risk score, there were significantly more post-operative pancreatic fistulae in the no glue cohort. There was no mortality in either group. Conclusion This study shows that application of sealant glue significantly reduces post-operative pancreatic fistulae in high-risk patients, but does not reduce overall complications or hospital stay following pancreaticoduodenectomy.

  4. The Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Their Conditioned Media Topically Delivered in Fibrin Glue on Chronic Wound Healing in Rats.

    Science.gov (United States)

    Mehanna, Radwa A; Nabil, Iman; Attia, Noha; Bary, Amany A; Razek, Khalid A; Ahmed, Tamer A E; Elsayed, Fatma

    2015-01-01

    Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent a modern approach for management of chronic skin injuries. In this work, we describe BM-MSCs application versus their conditioned media (CM) when delivered topically admixed with fibrin glue to enhance the healing of chronic excisional wounds in rats. Fifty-two adult male rats were classified into four groups after induction of large-sized full-thickness skin wound: control group (CG), fibrin only group (FG), fibrin + MSCs group (FG + SCs), and fibrin + CM group (FG + CM). Healing wounds were evaluated functionally and microscopically. Eight days after injury, number of CD68+ macrophages infiltrating granulation tissue was considerably higher in the latter two groups. Although--later--none of the groups depicted a substantially different healing rate, the quality of regenerated skin was significantly boosted by the application of either BM-MSCs or their CM both (1) structurally as demonstrated by the obviously increased mean area percent of collagen fibers in Masson's trichrome-stained skin biopsies and (2) functionally as supported by the interestingly improved epidermal barrier as well as dermal tensile strength. Thus, we conclude that topically applied BM-MSCs and their CM-via fibrin vehicle--could effectively improve the quality of healed skin in chronic excisional wounds in rats, albeit without true acceleration of wound closure.

  5. The Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Their Conditioned Media Topically Delivered in Fibrin Glue on Chronic Wound Healing in Rats

    Science.gov (United States)

    Mehanna, Radwa A.; Nabil, Iman; Attia, Noha; Bary, Amany A.; Razek, Khalid A.; Ahmed, Tamer A. E.; Elsayed, Fatma

    2015-01-01

    Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent a modern approach for management of chronic skin injuries. In this work, we describe BM-MSCs application versus their conditioned media (CM) when delivered topically admixed with fibrin glue to enhance the healing of chronic excisional wounds in rats. Fifty-two adult male rats were classified into four groups after induction of large-sized full-thickness skin wound: control group (CG), fibrin only group (FG), fibrin + MSCs group (FG + SCs), and fibrin + CM group (FG + CM). Healing wounds were evaluated functionally and microscopically. Eight days after injury, number of CD68+ macrophages infiltrating granulation tissue was considerably higher in the latter two groups. Although—later—none of the groups depicted a substantially different healing rate, the quality of regenerated skin was significantly boosted by the application of either BM-MSCs or their CM both (1) structurally as demonstrated by the obviously increased mean area percent of collagen fibers in Masson's trichrome-stained skin biopsies and (2) functionally as supported by the interestingly improved epidermal barrier as well as dermal tensile strength. Thus, we conclude that topically applied BM-MSCs and their CM—via fibrin vehicle—could effectively improve the quality of healed skin in chronic excisional wounds in rats, albeit without true acceleration of wound closure. PMID:26236740

  6. The Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Their Conditioned Media Topically Delivered in Fibrin Glue on Chronic Wound Healing in Rats

    Directory of Open Access Journals (Sweden)

    Radwa A. Mehanna

    2015-01-01

    Full Text Available Bone marrow-derived mesenchymal stem cells (BM-MSCs represent a modern approach for management of chronic skin injuries. In this work, we describe BM-MSCs application versus their conditioned media (CM when delivered topically admixed with fibrin glue to enhance the healing of chronic excisional wounds in rats. Fifty-two adult male rats were classified into four groups after induction of large-sized full-thickness skin wound: control group (CG, fibrin only group (FG, fibrin + MSCs group (FG + SCs, and fibrin + CM group (FG + CM. Healing wounds were evaluated functionally and microscopically. Eight days after injury, number of CD68+ macrophages infiltrating granulation tissue was considerably higher in the latter two groups. Although—later—none of the groups depicted a substantially different healing rate, the quality of regenerated skin was significantly boosted by the application of either BM-MSCs or their CM both (1 structurally as demonstrated by the obviously increased mean area percent of collagen fibers in Masson’s trichrome-stained skin biopsies and (2 functionally as supported by the interestingly improved epidermal barrier as well as dermal tensile strength. Thus, we conclude that topically applied BM-MSCs and their CM—via fibrin vehicle—could effectively improve the quality of healed skin in chronic excisional wounds in rats, albeit without true acceleration of wound closure.

  7. Characteristics of an autologous leukocyte and platelet-rich fibrin patch intended for the treatment of recalcitrant wounds

    DEFF Research Database (Denmark)

    Lundquist, Rasmus; Holmstrøm, Kim; Clausen, Christian

    2012-01-01

    of chronic wound fluid. By comparison with traditional platelet-rich plasma, differences in immune components were found. The relevance of these findings was assessed by showing a mitogenic and migratory effect on cultured human dermal fibroblasts. Further, we showed that fibrocytes, a cell type important......We have investigated the physical, biochemical, and cellular properties of an autologous leukocyte and platelet-rich fibrin patch. This was generated in an automated device from a sample of a patient's blood at the point of care. Using microscopy, cell counting, enzyme-linked immunosorbent assay...

  8. The mechanical and biological studies of calcium phosphate cement-fibrin glue for bone reconstruction of rabbit femoral defects

    Directory of Open Access Journals (Sweden)

    Dong J

    2013-03-01

    Full Text Available Jingjing Dong,1,* Geng Cui,2,* Long Bi,1,* Jie Li,3 Wei Lei11Institute of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, People’s Republic of China; 2Institute of Orthopedics, General Hospital of PLA, Beijing, People’s Republic of China; 3Institute of Gynecology and Obstetrics, General Hospital of PLA, Beijing, People’s Republic of China*These authors contributed equally to this workAbstract: In order to improve the mechanical and biological properties of calcium phosphate cement (CPC, nanometer-biomaterial for bone reconstruction in the rabbit femoral defect model, fibrin glue (FG, the natural product, purified from the blood was introduced at three different ratios. The CPC powder and the FG solution were mixed, respectively, at the powder/liquid (P/L ratios (g/mL of 1:1, 3:1, and 5:1 (g/mL, and pure CPC was used as a control. After being implanted into the femoral defect in rabbit, the healing process was evaluated by micro-computed tomography scan, biomechanical testing, and histological examination. By micro-computed tomography analysis, the P/L ratio of 1:1 (g/mL group indicated the largest quantity of new bone formation at 4 weeks, 8 weeks, and 12 weeks after implantation, respectively. Bone volume per trabecular volume of the 1:1 group was highest in the four groups, which was 1.45% ± 0.42%, 7.35% ± 1.45%, and 29.10% ± 1.67% at 4 weeks, 8 weeks, and 12 weeks after the operation, respectively. In the biomechanical tests, the compressive strength and the elastic modulus of the three CPC–FG groups were much higher than those of the pure CPC group at the determined time point (P < 0.05. The histological evaluation also showed the best osseointegration in the 1:1 group at 4 weeks, 8 weeks, and 12 weeks after the operation, respectively. In the 1:1 group, the bone grew into the pore of the cement in the laminar arrangement and connected with the cement tightly at the 12th week after the operation

  9. Application of fibrin glue in orthopedics%纤维蛋白胶在骨科中的应用

    Institute of Scientific and Technical Information of China (English)

    张飞; 高辉

    2012-01-01

    背景:纤维蛋白胶是一种生物蛋白制剂,由纤维蛋白原、凝血酶及其他生物活性因子组成,具有止血、趋化及促细胞有丝分裂等特性,有着良好的生物相容性.目的:对纤维蛋白胶在骨科的应用进行综述.方法:以"fibrin glue,biomaterial,application" 为英文检索词,以"纤维蛋白胶,骨科,应用" 为中文检索词,检索PubMed、BP、SD、万方及维普等数据库1995-01/2011-05相关文献,选取纤维蛋白胶在骨科中的应用有关文献30篇进行总结.结果与结论:纤维蛋白胶是一种具有多向生物活性的生物蛋白,能迅速形成立体网状结构,起到良好的止血作用.在注射部位局部形成凝胶圈,起封闭与粘合作用.具有预防脊柱手术后硬脊膜外腔粘连,抑制局部炎症扩散,减轻水肿压迫,促进术后切口愈合的作用.能充当生物基质,应用于骨或软骨组织工程.

  10. Effect of platelet-rich plasma and fibrin glue on healing of critical-size calvarial bone defects.

    Science.gov (United States)

    Findikcioglu, Kemal; Findikcioglu, Fulya; Yavuzer, Reha; Elmas, Cigdem; Atabay, Kenan

    2009-01-01

    Despite the insufficient number of experimental studies, platelet-rich plasma (PRP) including high amounts of growth factors is introduced to clinical use rapidly. The aim of this study was to compare the effects of PRP and platelet-poor plasma (PPP) on healing of critical-size bone defects.Bilateral full-thickness, critical-size bone defects were created in the parietal bones of 32 rabbits, which had been studied in 4 groups. Saline, thrombin solution, PPP, and PRP were applied to the created defects before closure. Radiologic defect area measurement results at 0, 4, and 16 weeks were compared between the groups. In addition, densities of the newly formed bones at 16th week were studied. Histologic parameters (primary and secondary bone trabecula, neovascularization, and bone marrow and connective tissue formation) were compared between 4- and 16-week groups.More rapid decrease in defect size was observed in groups 3 and 4 than in groups 1 and 2, both in the 4th and 16th weeks. Newly formed bone densities were also found to be higher in these 2 groups. New bone formation was detected to be more rapid considering histologic parameters, in groups 3 and 4 at 4th and 16th weeks.Study demonstrates that PRP and PPP might have favorable effects on bone healing. Although we cannot reveal any statistical difference between these 2 substances considering osteoinductive potential, PRP group has demonstrated superior results compared with fibrin glue group. Higher platelet concentrations may expose beneficial effects of PRP.

  11. Treatment of life-threatening wounds with a combination of allogenic platelet-rich plasma, fibrin glue and collagen matrix, and a literature review.

    Science.gov (United States)

    Asadi, Mehdi; Alamdari, Daryoush Hamidi; Rahimi, Hamid Reza; Aliakbarian, Mohsen; Jangjoo, Ali; Abdollahi, Abbas; Bahar, Mostafa Mehrabi; Azadmand, Ali; Forghani, Naser; Sadegh, Mohammad Nori; Khayamy, Mohammad Esmail; Seifalian, Alexander

    2014-08-01

    Currently there is no ideal procedure for the treatment of recalcitrant ulcers that are unresponsive to the majority of common treatments. However, several novel approaches have been proposed, including bone marrow stem cells, platelets, fibrin glue and collagen matrix. For the first approach treatment of a chronic wound, a non-invasive method is highly desirable. The present study was undertaken with the aim of evaluating the effect of a combination of platelets, fibrin glue and collagen matrix (PFC) in one treatment. A total of ten patients with aggressive, refractory, life-threatening wounds were recruited for the study and their treatment effects were evaluated. Initially, the ulcers were extensively debrided, measured and photographed at weekly intervals. The PFC combination was applied topically to the wound every two days. Following treatment, the wound was completely closed in nine patients and was markedly reduced in the other patient. The mean 100% healing time for the nine patients was 11.3±5.22 weeks. There was no evidence of local or systemic complications or any abnormal tissue formation, keloid or hypertrophic scarring. Therefore, the results of the present study indicate that in the first approach, the combination of PFC components may be used safely in order to synergize the effect of chronic wound healing.

  12. Autologous platelet gel and fibrin sealant enhance the efficacy of total knee arthroplasty : improved range of motion, decreased length of stay and a reduced incidence of arthrofibrosis

    NARCIS (Netherlands)

    Everts, Peter A. M.; Devilee, Roger J. J.; Oosterbos, Cornelis J. M.; Mahoney, Christine Brown; Schattenkerk, Maarten Eeftinck; Knape, Johannes T. A.; van Zundert, Andre

    2007-01-01

    In this study we describe the potential role of autologous platelet gel and fibrin sealant in unilateral total knee arthroplasty to improve the postoperative range of motion and to reduce the incidence of arthrofibrosis. Total knee arthroplasty is often associated with a considerable amount of post-

  13. A pilot study to evaluate the safety and clinical performance of Leucopatch, an autologous, additive-free, platelet-rich fibrin for the treatment of recalcitrant chronic wounds

    DEFF Research Database (Denmark)

    Jørgensen, Bo; Karlsmark, Tonny; Vogensen, Hanne;

    2011-01-01

    This prospective, uncontrolled pilot study evaluated the safety and clinical performance of Leucopatch an additive-free, autologous platelet-rich fibrin in the treatment of recalcitrant chronic wounds. Fifteen patients, with 16 lower extremity chronic wounds of varying etiologies were treated...

  14. Effect of topical autologous platelet-rich fibrin versus no intervention on epithelialization of donor sites and meshed split-thickness skin autografts: a randomized clinical trial

    DEFF Research Database (Denmark)

    Danielsen, P.; Jorgensen, B.; Jorgensen, L.N.;

    2008-01-01

    BACKGROUND: Autologous platelet-rich fibrin contains multiple growth factors. The aim of this randomized clinical trial was to study the effect of topical platelet-rich fibrin on epithelialization of donor sites and meshed split-thickness skin autografts. METHODS: Twenty consecutive leg ulcer...... surgically revised leg ulcer were randomized by concealed allocation to platelet-rich fibrin, and the other donor wound and autografted half were not (control). Biopsy specimens (4 mm) from donor wounds were evaluated for percentage neoepidermal coverage in tissue sections immunostained for keratin on days 5...... and 8. Epithelial barrier function, macroscopic healing, microbiology, and pain at dressing removal were assessed. Epithelialization of meshed autografts was assessed macroscopically. RESULTS: Epithelial coverage of donor wounds did not differ significantly between platelet-rich fibrin and control...

  15. Application of medical fibrin glue and stitch in pterygium excision:a Meta-analysis%医用生物蛋白胶与缝线在翼状胬肉切除中应用的Meta分析

    Institute of Scientific and Technical Information of China (English)

    郭子君; 王微

    2015-01-01

    BACKGROUND:Currently, in pterygium excision combined with limbal epithelial transplantation, there were stil some controversies about whether the medical fibrin glue could instead of stitch fixing autologous conjunctival graft. OBJECTIVE:To compare the therapeutic effect of medical fibrin glue and stitch in pterygium excision combined with limbal epithelial transplantation using Meta analysis. METHODS: The randomized controled trials about the therapeutic effect of medical fibrin glue versus stitch in pterygium excision combined with limbal epithelial transplantation were reviewed from PubMed database, CNKI ful-text database, Chinese Biomedical Literature Database, VIP database and Wanfang database from database creation to June 2015. The operative time, complications and recurrence rate of these two groups were analyzed using Meta. RESULTS AND CONCLUSION:A total of 14 randomized controled trials, and 879 patients were enroled. Meta-analysis results showed that the operative time, incidence of postoperative complications, the relapse rate after six months and one-year in the fibrin glue group were lower than those in the suture group [MD=-15.58, 95%CI (-18.96,-12.21),P < 0.000 01]; [OR=0.39, 95%CI(0.19, 0.68),P=0.02]; [OR=0.28, 95%CI(0.12, 0.65),P=0.003]; [OR=0.23, 95%CI(0.07, 0.76),P=0.02]. These results demonstrate that the application of fibrin glue in pterygium excision combined with limbal stem cel transplantation can promote graft attachment, shorten the operation time, reduce postoperative complications and reduce the postoperative recurrence of pterygium.%背景:目前在翼状胬肉切除联合角膜缘上皮移植中,对于医用生物蛋白胶能否代替缝线固定自体球结膜植片还存在一定争议。目的:采用Meta分析比较医用生物蛋白胶与缝线在翼状胬肉切除联合角膜缘上皮移植中应用的效果。方法:查阅 PubMed 数据库、CNKI 全文数据库、中国生物医学文献数据库、维普数据库和

  16. Recombinant human bone morphogenetic protein-2 suspended in fibrin glue enhances bone formation during distraction osteogenesis in rabbits

    Science.gov (United States)

    Li, Yunfeng; Li, Rui; Hu, Jing; Song, Donghui; Jiang, Xiaowen

    2016-01-01

    Introduction Bone morphogenetic protein-2 (BMP-2) has high potential for bone formation, but its in vivo effects are unpredictable due to the short life time. This study was designed to evaluate the effects of recombinant human (rh) BMP-2 suspended in fibrin on bone formation during distraction osteogenesis (DO) in rabbits. Material and methods The in vitro release kinetics of rhBMP-2 suspended in fibrin was tested using an enzyme-linked immunosorbent assay. Unilateral tibial lengthening for 10 mm was achieved in 48 rabbits. At the completion of osteodistraction, vehicle, fibrin, rhBMP-2 or rhBMP-2 suspended in fibrin (rhBMP-2 + fibrin) was injected into the center of the lengthened gap, with 12 animals in each group. Eight weeks later, the distracted callus was examined by histology, micro-CT and biomechanical testing. Radiographs of the distracted tibiae were taken at both 4 and 8 weeks after drug treatment. Results It was found that fibrin prolonged the life span of rhBMP-2 in vitro with sustained release during 17 days. The rhBMP-2 + fibrin treated animals showed the best results in bone mineral density, bone volume fraction, cortical bone thickness by micro-CT evaluation and mechanical properties by the three-point bending test when compared to the other groups (p < 0.05). In histological images, rhBMP-2 + fibrin treatment showed increased callus formation and better gap bridging compared to the other groups. Conclusions The results of this study suggest that fibrin holds promise to be a good carrier of rhBMP-2, and rhBMP-2 suspended in fibrin showed a stronger promoting effect on bone formation during DO in rabbits. PMID:27279839

  17. Management of an endo-perio lesion in an immature tooth using autologous platelet-rich fibrin: A case report

    Directory of Open Access Journals (Sweden)

    N B Nagaveni

    2015-01-01

    Full Text Available Treatment of an endo-perio lesion involving a non-vital young permanent tooth is a highly challenging task to Pediatric Dentists. There is a quest for the newer biological approach to management of these lesions as traditional methods have various disadvantages. Recently, platelet-rich fibrin (PRF, a second-generation platelet concentrate, is rich in growth factors have been used in the periodontal regeneration procedure. The purpose of this paper is to describe the efficacy of PRF in the treatment of a deep intra bony defect associated with an endo-perio lesion in an immature right mandibular first premolar of 12-year-old female patient. A freshly prepared autologous PRF membrane was placed in the bony defect following debridement. Clinical and radiographic follow-up were performed at regular intervals that revealed absence of pain, gain in clinical attachment level, reduction in probing depth, and excellent bone regeneration indicating successful outcome.

  18. Management of an endo-perio lesion in an immature tooth using autologous platelet-rich fibrin: a case report.

    Science.gov (United States)

    Nagaveni, N B; Kumari, K Nandini; Poornima, P; Reddy, V V Subba

    2015-01-01

    Treatment of an endo-perio lesion involving a non-vital young permanent tooth is a highly challenging task to Pediatric Dentists. There is a quest for the newer biological approach to management of these lesions as traditional methods have various disadvantages. Recently, platelet-rich fibrin (PRF), a second-generation platelet concentrate, is rich in growth factors have been used in the periodontal regeneration procedure. The purpose of this paper is to describe the efficacy of PRF in the treatment of a deep intra bony defect associated with an endo-perio lesion in an immature right mandibular first premolar of 12-year-old female patient. A freshly prepared autologous PRF membrane was placed in the bony defect following debridement. Clinical and radiographic follow-up were performed at regular intervals that revealed absence of pain, gain in clinical attachment level, reduction in probing depth, and excellent bone regeneration indicating successful outcome.

  19. Characteristics of an autologous leukocyte and platelet-rich fibrin patch intended for the treatment of recalcitrant wounds.

    Science.gov (United States)

    Lundquist, Rasmus; Holmstrøm, Kim; Clausen, Christian; Jørgensen, Bo; Karlsmark, Tonny

    2013-01-01

    We have investigated the physical, biochemical, and cellular properties of an autologous leukocyte and platelet-rich fibrin patch. This was generated in an automated device from a sample of a patient's blood at the point of care. Using microscopy, cell counting, enzyme-linked immunosorbent assay, antibody arrays, and cell culture assays, we show that the patch is a three-layered membrane comprising a fibrin sheet, a layer of platelets, and a layer of leukocytes. Mean recovery of platelets from the donated blood was 98% (±95%CI 0.8%). Mean levels of platelet-derived growth factor AB, human transforming growth factor beta 1, and vascular endothelial growth factor extracted from the patch were determined as 127 ng (±95% CI 20), 92 ng (±95%CI 17), and 1.35 ng (±95%CI 0.37), respectively. We showed a continued release of PDGF-AB over several days, the rate of which was increased by the addition of chronic wound fluid. By comparison with traditional platelet-rich plasma, differences in immune components were found. The relevance of these findings was assessed by showing a mitogenic and migratory effect on cultured human dermal fibroblasts. Further, we showed that fibrocytes, a cell type important for acute wound healing, could be grown from the patch. The relevance of these findings in relation to the use of the patch for treating recalcitrant wounds is discussed.

  20. 纤维蛋白胶在泌尿外科手术中的应用%Application of fibrin glue in urological surgical procedures

    Institute of Scientific and Technical Information of China (English)

    高彦俊; 许丽丽

    2014-01-01

    纤维蛋白胶(fibrin glue,FG)是一种生物蛋白制剂,兼具止血、组织粘合、封闭组织缺损和促进组织愈合的功能.其止血效果良好,且具有良好的生物相容性和可降解性,已在普外科、骨科、心血管外科、整形外科、泌尿外科等众多科室应用并取得了较好的疗效.本文对FG在泌尿外科手术中的应用进展作一综述.

  1. The adjuvant use of stromal vascular fraction and platelet-rich fibrin for autologous adipose tissue transplantation.

    Science.gov (United States)

    Liu, Bin; Tan, Xin-Ying; Liu, Yan-Pu; Xu, Xiao-Fang; Li, Long; Xu, Hai-Yan; An, Ran; Chen, Fa-Ming

    2013-01-01

    Autologous adipose transplantation is rapidly gaining popularity for the restoration of soft tissue defects and lipoatrophy as well as for aesthetic improvements (e.g., facial reconstruction and rejuvenation). However, the current technique is crude that suffers from serious demerits, particularly the long-term unpredictability of volume maintenance due to resorption of the grafted adipose tissue and limited adipogenesis. We hypothesized that the adjuvant use of patient-derived adipose stromal vascular fraction (SVF) and platelet-rich fibrin (PRF) may enhance the overall outcome of autologous fat grafting in plastic and reconstructive surgery. Autologous SVF, with a mean cell number of (4.8±3.79)×10⁷ cells/mL and a mean cell viability of 71.8%, and autologous PRF, with sustained release of multiple angiogenic growth factors, were created before surgical use. The following adipose tissue implants were injected subcutaneously into a rabbit ear's auricula according to the following study design: 2 mL adipose granules and 0.2 mL normal saline solution (AG+NS group), 2 mL adipose granules and 0.2 mL SVF (AG+SVF group), 2 mL adipose granules and 0.2 mL PRF (AG+PRF group), or 2 mL adipose granules combined with 0.1 mL SVF and 0.1 mL PRF (AG+SVF+PRF group). Histological examinations showed that the implanted adipose granules were well engrafted in the AG+SVF+PRF group, with a higher microvessel density 4 weeks postimplantation compared with the other three groups (p<0.01). Twenty-four weeks postimplantation, the resorption rates of implanted tissue in each group were 49.39%±9.47%, 27.25%±4.37%, 36.41%±8.47%, and 17.37%±6.22%, respectively, and were significantly different (p<0.01). The results demonstrated that the efficacy of adipose tissue implantation can be enhanced by using autologous PRF and SVF as therapeutic adjuvants, offering a clinically translatable strategy for soft tissue augmentation and reconstruction.

  2. 纤维蛋白胶在软组织工程中的应用%Application of fibrin glue in soft tissue engineering

    Institute of Scientific and Technical Information of China (English)

    徐颖; 宋岩峰

    2011-01-01

    Fibrin glue is mainly composed of natural extracellular matrix and widely applied to tissue engineering research as scaffold recently because of its good biocompatibility,effective bioactivity,biodegradability,fine plasticity,prepared convenience,as well as having three dimensional porous structure and without cytotoxicity.Fibrin alone or in combination with other materials has been used as a biological scaffold for stem or primary cells to regenerate adipose tissue,bone,cardiac tissue,cartilage,liver,nervous tissue,ocular tissue,skin,tendons,and ligaments.Thus,fibrin is a versatile biopolymer,which shows a great potential in tissue engineering.%纤维蛋白胶是一种天然的细胞外基质,其生物相容性、生物活性、可降解性、可塑性良好,具有三维多孔结构,无细胞毒性,制备方便;近年来,纤维蛋白胶作为支架材料广泛地应用到组织工程研究中。纤维蛋白胶自身或与其他材料结合作为生物支架可应用于干细胞、始祖细胞再生修复脂肪组织、骨、心肌组织、软骨、肝、神经组织、眼、皮肤、肌腱及韧带组织。因此,纤维蛋白胶作为多用途的生物多聚体,在组织工程应用上具巨大潜力。

  3. Anastomose colônica com adesivo de fibrina em ratos diabéticos Colonic anastomosis with fibrin glue in diabetic rats

    Directory of Open Access Journals (Sweden)

    Orlando Ribeiro Prado Filho

    2008-10-01

    Full Text Available OBJETIVO: Estudar aspectos da cicatrização na anastomose colônica com adesivo de fibrina em ratos normais e diabéticos. MÉTODO: Foram utilizados 160 ratos Wistar (Rattus novergicus albinus, machos, com peso variando de 250 a 300g, sendo que oitenta destes animais eram não diabéticos e os outros oitenta animais apresentavam diabetes mellitus induzido pela aloxana, Os animais foram submetidos a procedimento operatório padronizado que consistiu em colectomia parcial esquerda e anastomose primária por sutura manual ou com adesivo de fibrina. Foram reavaliados no quarto ou sétimo dia de pós-operatório quando se observou a presença de coleção líquida ou purulenta na tela subcutânea; integridade da sutura; presença de aderências; presença de deiscência; resistência da anastomose (pressão máxima de insuflação em mmHg, e a concentração tecidual de hidroxiprolina. RESULTADOS: Em relação à presença de deiscência de anastomose e mortalidade ocorreram piores resultados nos animais diabéticos no quarto e sétimo dias de observação em comparação ao controle. Nos grupos e subgrupos estudados os resultados com o uso do adesivo de fibrina não foram melhores que os da sutura manual. Em relação à concentração tecidual de hidroxiprolina não houve diferença significativa entre os grupos. CONCLUSÃO: O diabetes mellitus induzido pela aloxana impede a reparação normal da anastomose colônica em ratos e o uso adesivo de fibrina na anastomose colônica em ratos normais e diabéticos não trouxe benefícios na evolução pós-operatória.BACKGROUND: The aim of this experimental study was to assess healing in colonic anastomosis with fibrin glue in normal and diabetics rats. METHODS: One hundred and sixty Wistar rats were randomly assigned for two groups (normal and diabetics. The animals underwent a partial left colectomy and after an end-to-end anastomosis or a fibrin glue anastomosis. The rats were submitted to a new

  4. Utilização de adesivo de fibrina em cirurgias oftalmológicas Use of fibrin glue in ocular surgery

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    Melissa Manfroi Dal Pizzol

    2009-06-01

    Full Text Available OBJETIVO: Avaliar as diferentes técnicas cirúrgicas oftalmológicas nas quais se pode utilizar a cola biológica e possíveis complicações relacionadas ao seu uso. MÉTODOS: Estudo de série de casos retrospectivo, não-comparativo. Foram avaliados 161 olhos de pacientes com idade média de 51,8 anos em que foi utilizada cola biológica (Beriplast P® - Aventis Behring, Alemanha para fixação de tecido ou fechamento incisional. RESULTADOS: Foram avaliados 76 pacientes com pterígio, 4 com ceratoconjuntivite límbica superior, 4 com tumor limbar, 21 com conjuntivocálase, 31 com ceratopatia bolhosa, 7 com deficiência límbica, 6 com queimadura alcalina, 2 com Stevens-Johnson, 5 com catarata e 5 com glaucoma. Complicações foram relatas em 28 casos (17,4%. Descolamento do tecido fixado e granuloma foram as complicações mais frequentes. Descolamento ocorreu em 10 casos (5,6% (7 em transplante de membrana amniótica em ceratopatia bolhosa, 2 em transplante de conjuntiva autólogo após exérese de pterígio, 1 após transplante de limbo autólogo por Stevens-Johnson. Granuloma ocorreu em 9 casos (5,6% (5 em pacientes com queimadura alcalina aguda tratados com transplante de membrana amniótica, 1 em paciente com sequela de queimadura tratado com transplante de limbo autólogo e 3 em pacientes após exérese de pterígio tratados com transplante de conjuntiva autólogo. Todas as complicações ocorreram dentro da primeira semana de pós-operatório. CONCLUSÃO: Embora o presente seja retrospectivo e não-comparativo, podemos observar que a utilização do adesivo de fibrina parece ser segura e efetiva, além de apresentar baixas taxas de complicações nas cirurgias de superfície ocular e de segmento anterior.PURPOSE: To evaluate the use of fibrin glue and its complications in different ophthalmic surgeries. METHODS: A retrospective and non-comparative case series study. 161 eyes were evaluated. The mean age was 51.8 years. Fibrin glue

  5. End-to-side nerve repair using fibrin glue in rats Anastomose nervosa término-lateral usando cola de fibrina em ratos

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    Daniel Nunes e Silva

    2010-04-01

    Full Text Available PURPOSE: To evaluate axonal regeneration after end-to-side nerve repair with fibrin glue in rats. METHODS: Forty-five Wistar rats were divided into three groups: group A (n=15, were not submitted to surgery (control group; group B (n=15 were submitted to fibular transection without repair; and group C (n=15, were submitted to fibular transection with end-to-side nerve anastomosis using fibrin glue, in the lateral surface of an intact tibial nerve. The three groups were submitted to walking track (30 and 90 days and posterior morphometrical analysis (90 days. RESULTS: The functional tests demonstrated that there was no difference in the walking track during the study in group A (p>0.05. The group B had walking pattern impairment in the two tests (p>0.05. The group C had walking pattern impairment in the first test, with important recovery in the second test (pOBJETIVO: Avaliar a regeneração axonal após anastomose nervosa término-lateral (ATL usando cola de fibrina em ratos. MÉTODOS: Quarenta e cinco ratos Wistar distribuídos em três grupos: os animais do grupo A (n=15 não foram submetidos à secção nervosa (grupo controle; os animais do grupo B (n=15 foram submetidos apenas à secção do nervo fibular, sem posterior anastomose; e os animais do grupo C (n=15 foram submetidos à secção do nervo fibular e à ATL com cola de fibrina no nervo tibial. Posteriormente, os animais foram submetidos a dois testes de marcha (30 e 90 dias e à análise morfométrica (90 dias. RESULTADOS: A análise estatística dos testes de marcha demonstrou que o grupo A não apresentou alteração no padrão de caminhada durante o estudo (p>0,05. O grupo B apresentou prejuízo motor no primeiro e no segundo teste (p>0,05. O grupo C apresentou um padrão de atrofia no primeiro teste, com recuperação da marcha no segundo teste (p<0,05. Na análise morfométrica, o grupo C apresentou regeneração axonal significativamente superior ao grupo B (p<0,05. CONCLUS

  6. Application of fibrin glue with bandage contact lens in pterygium surgery%纤维蛋白胶联合绷带型角膜接触镜在翼状胬肉切除术中的应用

    Institute of Scientific and Technical Information of China (English)

    刘伟; 张映萍; 谢梅芬; 刘妍

    2014-01-01

    目的:探讨应用纤维蛋白胶联合绷带型角膜接触镜减轻翼状胬肉切除联合自体角膜缘干细胞移植术后疼痛的效果。  方法:对52例72眼行翼状胬肉切除联合自体角膜缘干细胞移植手术治疗的初发性翼状胬肉患者,随机分为试验组(纤维蛋白胶联合绷带型角膜接触镜组)28例38眼和对照组(缝线组)24例34眼。试验组及对照组给予常规20 g/L利多卡因浸润麻醉后进行翼状胬肉切除联合自体角膜缘干细胞移植术。试验组采用黏合剂将植片与植床进行粘贴,对照组采用10-0缝线缝合植片与植床。术毕,试验组角膜表面放置角膜接触镜,对照组不配戴角膜接触镜。主要术后观察患者的术后1,2,3,5,7 d疼痛评分。术后随访6mo,观察植床与植片的匹配程度及感染、复发、植片愈合不良、结膜下囊肿等并发症情况。  结果:试验组患者疼痛程度评分均较对照组显著降低(均为P=0.000)。观察期内,两组植床与植片均成功固定于巩膜表面,并全部成活,同时两组患者均未发生感染、复发、植片愈合不良、结膜下囊肿等并发症。  结论:联合运用纤维蛋白胶黏合剂粘及角膜接触镜,可明显减轻翼状胬肉术后的疼痛反应。%AIM:To explore the efficacy of fibrin glue with bandage contact lens for pain relief after pterygium surgery performed with limbal autograft transplantation. METHODS:A prospective clinical trial was carried out in 52 patients ( 72 eyes ) operated for primary nasal pterygium. All patients were randomly divided into the fibrin glue with bandage contact lens group ( experimental group, 28 cases, 38 eyes ) and suture group ( control group, 24 cases, 34 eyes) . Autologous limbal graft taken from the superotemporal limbus was used to cover the sclera after pterygium excision under local anesthesia with 20g/L lidocaine. In experimental group, the transplant was attached to the sclera with

  7. 纤维蛋白胶运用于翼状胬肉手术的临床观察%Clinical observation on fibrin glue application during pterygium surgery

    Institute of Scientific and Technical Information of China (English)

    雷琦峰; 蔡维

    2015-01-01

    目的::观察纤维蛋白胶运用于翼状胬肉切除联合自体结膜移植术的临床效果。方法:选取60例60眼原发性鼻侧翼状胬肉患者随机分为试验组(纤维蛋白胶组)和对照组(缝线组)各30例30眼。行翼状胬肉切除联合自体结膜移植术,试验组采用纤维蛋白胶粘合固定植片,对照组采用10-0尼龙线缝合固定植片。术后随访6mo,观察手术时间、术后疼痛、异物感、并发症以及复发。结果:试验组手术时间(24.5±6.5min)较对照组(35.2±5.4min)短,两组差异有统计学意义(P<0.05)。术后患者疼痛和异物感试验组较对照组减轻(P<0.05)。两组均未发生术后严重并发症,试验组结膜下出血发生率低于控制组(P<0.05),6mo时纤维蛋白胶组1例(3%)复发,缝线组3例(10%)复发。结论:纤维蛋白胶运用于翼状胬肉手术固定结膜植片能减轻患者术后不适,减少手术时间及术后并发症,是一种安全有效的方法。%AlM: To observe the clinical results of pterygium excision combined with conjunctival autograft transplantation using fibrin glue.METHODS:A total of 60 patients (60 eyes) with primary nasal pterygium were randomly divided into two groups:the fibrin glue group ( experimental group, 30 eyes) and suture group ( control group, 30 eyes ) . All patients underwent pterygium excision combined with conjunctival autograft transplantation. ln the experimental group autograft was attached to sclera with fibrin glue while in control group 10-0 polyamide was used. The patients were followed up for 6mo. The time of operation, post operation comfort, complications and recurrence were evaluated.RESULTS:The average surgical time was 24. 5 ± 6. 5min with fibrin glue group while 35. 2 ± 5. 4min with suture group, with statistically significant difference between two groups (P<0. 05). Pain and foreign body sensation was significantly less with fibrin glue group than that in the control group ( P < 0. 05 ). No

  8. Application of biomedical fibrin glue in the cleft palate surgery%医用生物蛋白胶在腭裂修复术中的应用

    Institute of Scientific and Technical Information of China (English)

    胡广伟; 廖天安

    2012-01-01

    目的:观察医用生物蛋白胶在腭裂修复术创口中的应用对伤口愈合效果的影响.方法:将已配制的医用生物蛋白胶喷涂在腭裂双侧松弛切口及裸露创面.结果:48例腭裂术后伤口愈合良好,未出现明显伤口出血、糜烂及穿孔.结论:生物蛋白胶可通过封闭创口促进伤口愈合,在腭裂整复术中应用可靠、安全.%Objective To observe the possible effect of biomedical fibrin glue (BFG) on the healing of wound after cleft palate surgery. Methods BFG was distributed on the bone surfaces and the lateral palate incisions after suturing the palate flaps. Results All cases got good healing without ulceration and perforation. Conclusion BFG is safe and reliable in the application of the cleft palate surgery by blocking operative wound and promoting healing.

  9. 生物蛋白胶在腭裂整复术中的应用%Application of Fibrin Glue in the Cleft Palate Surgery

    Institute of Scientific and Technical Information of China (English)

    郑俊发; 赵建江; 景向东

    2001-01-01

    Objective To observe the possible effect of Fibrin Glue(FG) onthe healing of wound after cleft palate surgery. Method FG was distributed on the bone surfaces and the lateral palate incisions after suturing the palate flaps. Results 28 of 30 cases got good healing, 2 caces occured ulceration in the area between hard and soft palate but no perforation. Conclusion FG can be appplied in the cleft palate surgery and it is reliable and safe.%目的 观察生物蛋白胶在腭裂整复术创口中的应用对伤口愈合效果的影响。方法 将已配制的生物蛋白胶涂在腭裂双侧松弛切口及裸露创面,达到完全封闭状态。结果 30例腭裂术后28例伤口愈合良好,无出现糜烂,2例于软硬腭交界处口腔粘膜侧有糜烂,但肌层及鼻腔侧粘膜层无裂开,未出现穿孔。结论 生物蛋白胶可通过封闭创口促进伤口愈合,在腭裂整复术中应用可靠、安全。

  10. Treatment of non-healing sternum wound after open-heart surgery with allogenic platelet-rich plasma and fibrin glue-preliminary outcomes

    Directory of Open Access Journals (Sweden)

    Mohammad Abbasi Tashnizi

    2013-01-01

    Full Text Available Introduction: Non-healing wound in the sternal region after coronary arteries bypass graft surgery is a serious complication. For healing a chronic wound, several novel approaches have been proposed recently such as using bone marrow stem cells, platelets and fibrin glue (PFG; but a non-invasive method is highly desirable in the first approach for treatment. The current study was undertaken to evaluate the effect of the combination of PFG in one treatment. Materials and Methods: We report on the treatment of six patients with life-threatening chronic sternum wounds, which caused septicemia with multi-drug resistant pathogens. The ulcers were extensively debrided initially and were measured and photographed at weekly intervals. The combination of PFG was applied topically on the wound after every 2 days. Results: The wounds were completely closed in five patients and significantly reduced in size in one. There was no evidence of local or systemic complications and any abnormal tissue formation, keloid or hypertrophic scarring. Conclusions: Our study suggests, in the first approach, PFG can be used safely in order to heal a non healing sternum wound following coronary artery bypass surgery.

  11. A pilot study to evaluate the safety and clinical performance of Leucopatch, an autologous, additive-free, platelet-rich fibrin for the treatment of recalcitrant chronic wounds

    DEFF Research Database (Denmark)

    Jørgensen, Bo; Karlsmark, Tonny; Vogensen, Hanne;

    2011-01-01

    This prospective, uncontrolled pilot study evaluated the safety and clinical performance of Leucopatch an additive-free, autologous platelet-rich fibrin in the treatment of recalcitrant chronic wounds. Fifteen patients, with 16 lower extremity chronic wounds of varying etiologies were treated...... events. Two adverse events, one of noncompliance and one infection, were observed; neither was considered to be related to treatment. The results indicate that Leucopatch is easy to prepare and apply in the clinic, is safe, and may be a clinically effective treatment of recalcitrant chronic wounds....

  12. Platelet-rich plasma and fibrin glue-coated bioactive ceramics enhance growth and differentiation of goat bone marrow-derived stem cells.

    Science.gov (United States)

    Nair, Manitha B; Varma, H K; John, Annie

    2009-07-01

    New biotechnologies such as tissue engineering require functionally active cells within supportive matrices where the physical and chemical stimulus provided by the matrix is indispensable to determine the cellular behavior. This study has investigated the influence of platelet-rich plasma (PRP) and fibrin glue (FG) on the functional activity of goat bone marrow-derived mesenchymal stem cells (gBMSCs) that differentiated into the osteogenic lineage. To achieve this goal, PRP and FG were separately coated on bioactive ceramics like hydroxyapatite (HA) and silica-coated HA (HASi), on which gBMSCs were seeded and induced to differentiate into the osteogenic lineage for 28 days. The cells were then analyzed for viability (lactate dehydrogenase assay: acridine orange and ethidium bromide staining), morphology (scanning electron microscopy), proliferation (picogreen assay), cell cycle assay (propidium iodide staining), and differentiation (alkaline phosphatase [ALP] activity and real-time PCR analysis of ALP, osteocalcin, and osteopontin gene). It has been observed that PRP and FG have appreciably favored the viability, spreading, and proliferation of osteogenic-induced gBMSCs. The osteopontin and osteocalcin expression was significantly enhanced on PRP- and FG-coated HA and HASi, but PRP had effect on neither ALP expression nor ALP activity. The results of this study have depicted that FG-coated ceramics were better than PRP-coated and bare matrices. Among all, the excellent performance was shown by FG coated HASi, which may be attributed to the communal action of the stimulus emanated by Si in HASi and the temporary extracellular matrix provided by FG over HASi. Thus, we can conclude that PRP or FG in combination with bioactive ceramics could possibly enhance the functional activity of cells to a greater extent, promoting the hybrid composite as a promising candidate for bone tissue engineering applications.

  13. 双套管冲洗引流联合蛋白胶封堵在肠外瘘的护理体会%Nursing Experience of Dual Flush Tube Drainage Combined with Fibrin Glue Plugging Enterocutaneous Fistula

    Institute of Scientific and Technical Information of China (English)

    郝玉英

    2014-01-01

    目的探讨肠外瘘患者应用双套管持续冲洗引流联合纤维蛋白胶封堵瘘管的护理特点。方法回顾性分析2010年1月至2013年1月我院收治的15例肠外瘘患者使用双套管持续冲洗引流联合纤维蛋白胶封堵瘘管的护理方法。结果通过有效的个体化阶段性和综合性护理,患者住院时间(20±4)d,保守治疗肠外瘘成功率93.3%。结论腹腔双套管持续冲洗引流联合纤维蛋白胶封堵肠外瘘成功率高,护理重点在于关注患者心理护理、双套管护理、瘘口周围皮肤护理。%Objective To investigate the application of dual flush tube drainage combined with fibrin glue in plugging en-terocutaneous fistula.Methods Retrospectivly analysis the nursing methods of dual flush tube drainage combined with fibrin glue plugging enterocutaneous fistula in 15 petients from January 2010 to January 2013 in our hospital.Results Through effective indi-vidual stages and comprehensive care, hospitalization time were (20±4)d,enterocutaneous fistula conservative treatment success rate of 93.3%.Conclusion Abdominal double cannula continuous irrigation and drainage combined with fibrin glue plugginh en-terocutaneous fistula is easily success,the nursing focus is pay attention to patients' psychological nursing、dual flush tube nursing snd the surrounding fistula skin care.

  14. Biocompatibility in vitro between fascia fibroblasts and fibrin glue%筋膜成纤维细胞与纤维蛋白凝胶的体外生物相容性

    Institute of Scientific and Technical Information of China (English)

    辛培成; 郭其勇; 李杰; 赵德伟; 杨圣

    2015-01-01

    BACKGROUND:Fibrin glue is a natural biodegradable scaffold, which can be used for tissue-engineered scaffolds, and is increasingly used as seed cel carrier for tissue engineering repair. OBJECTIVE:To investigate the biocompatibility in vitro of rabbit fascia fibroblasts and fibrin glue. METHODS:Tissue explants adherent method was used to culture fibroblasts from subcutaneous deep fascia tissue of New Zealand white rabbits. The fibroblasts could be passaged with trypsin digestion method. Suspension of passage four fibroblasts was co-cultured with fibrin glue. Morphology and proliferation of fibroblasts on the surface of fibrin glue were dynamical y observed under the inverted phase contrast microscope. At 5 days after co-culture, fibroblasts were identified by immunofluorescence staining under the laser scanning confocal microscope. The fibroblast growth and adhesion were observed under the scanning electron microscope. RESULTS AND CONCLUSION:There was no significant difference in fibroblast morphology between co-culture fibroblasts and pure culture fibroblasts with inverted phase contrast microscope. Scanning electron microscope demonstrated that fibroblasts ful y extended in fibrin glue surface, and showed a good adhesion between the“pseudopodium”and fibrin glue, and secreted matrix material. It is clear that the fibrin glue did not alter the morphologic features of fibroblasts. Laser scanning confocal microscope revealed that fibroblasts were positive for vimentin. These verified that properties of fibroblasts did not change after they were seeded in fibrin glue surface and did not be induced to differentiate. There is a very good biocompatibility between fascia fibroblasts and fibrin glue in vitro.%背景:纤维蛋白凝胶是一种天然可降解的生物支架材料,具备可用于组织工程支架的共性,被越来越多地用做种子细胞载体应用于组织工程修复。目的:观察兔筋膜成纤维细胞与纤维蛋白凝胶的体外

  15. 纤维蛋白胶抗体间接ELISA检测方法的建立及初步应用%Development and preliminary application of indirect ELISA method for fibrin glue antibody

    Institute of Scientific and Technical Information of China (English)

    蔡阳; 任真; 何学军; 吴华; 乔红群

    2012-01-01

    Objective To develop and preliminarily apply an indirect ELISA method for fibrin glue antibody. Methods An indirect ELISA method for fibrin glue antibody was developed using fibrin glue as coating antigen, of which the reaction condition was optimized, and precision and specificity were verfied. The serum antibody of rabbits immunized with fibrin glue was determined by the developed method. Results The reaction condition for the developed indirect ELJSA method was optimized as follows: microtiter plate was irradiated vertically with UV light for 20 min, then coated with fibrin glue at a concentration of 10 |xg/ml, diluted with 0. 05 mol/L bicarbonate solution (pH 9. 6) as coating buffer solution, at 4℃ overnight; the PBS containing 10% fetal bovine serum was served as blocking solution, while 0. 1% PBST as antibody diluent; the optimal dilutions of polyclonal antibody against fibrin glue and HRP-labeled goat anti-rabbit IgG were 1 : 2 500 and 1 : 4 000 respectively. The optimal temperature and time for reaction were 37℃ and 1 h, while those for substrate coloration were 37℃ and 15 min, respectively. The sulfuric acid at a concentration of 2 mol / L was served as stop solution. The method showed high precision and specificity. The antibodies in sera of rabbits 1,2,4 and 6 weeks after immunization were determined, and the result showed that the serum antibody level increased significantly 2 weeks while started to decrease 6 weeks after immunization. Conclusion An indirect ELISA method for fibrin glue antibody was successfully developed, which might be used for the determination of fibrin glue antidoy in immune sera of rabbits.%目的 建立纤维蛋白胶抗体间接ELISA检测方法,并进行初步应用.方法 采用纤维蛋白胶作为包被抗原,建立检测纤维蛋白胶抗体的间接ELISA法,优化反应条件,并对方法的精密性及特异性进行验证.采用建立的间接ELISA法检测纤维蛋白胶免疫兔血清抗体.结果 间接ELISA法

  16. Combined left hepatectomy with fenestration and using a harmonic scalpel, fibrin glue and closed suction drainage to prevent bile leakage and ascites in the management of symptomatic polycystic liver disease: a case report

    Directory of Open Access Journals (Sweden)

    Kosmidis Christopher

    2009-08-01

    Full Text Available Abstract Introduction Surgical treatment is the usual therapy for patients with polycystic liver disease and with severe symptoms, yet the results of surgery are often disappointing and the optimal surgical approach is uncertain. Case presentation We present the case of a 41-year-old Greek woman who underwent combined left hepatectomy with fenestration for symptomatic polycystic liver disease using ultrasound scalpel, fibrin glue and closed suction drain to prevent bile leakage, haemorrhage and ascites. Liver resection using the ultrasound scissors allowed quick parenchyma dissection under haemostatic conditions with safe coagulation of small vessels and bile ducts. Moreover, the ultrasound scalpel was applied to the cyst cavities exposed on the peritoneum to ablate the fluid-producing epithelial cyst lining. We also covered the cut cystic cavities exposed to the peritoneum surface of the liver with fibrin glue. Instead of allowing the opened cysts to drain into the abdominal cavity, we used two wide bore closed suction fluted drains. We did not observe excessive fluid loss through the drainage after the second postoperative day. The drain tubes were removed on the third postoperative day. Conclusion In our patient, effective treatment of ascites and prevention of bile leakage and bleeding indicate that this new approach is promising and may become a useful surgical technique for polycystic liver disease.

  17. Applications of absorbable fibrin glue and gelatin sponge in partial liver resection%可吸收材料生物蛋白胶及明胶海绵在肝部分切除的应用

    Institute of Scientific and Technical Information of China (English)

    何艳新; 刘超

    2012-01-01

    BACKGROUND: Fibrin glue is widely applied for blood oozing wounds in various surgeries. OBJECTIVE: To apply absorbable fibrin glue and gelatin sponge in partial liver resection so as to analyse the operation time and postoperative hemostasis effect. METHODS: Totally 42 patients undergoing partial liver resection were randomly divided into two groups: in the control group defects of liver tissue were folded after partial hepatectomy and hemostasis; in the experimental group, the wound was sprayed with fibrin glue to achieve hemostasis effect and the defect was filled with fibrin glue and gelatin sponge cutting into pieces to replace the conventional suture. RESULTS AND CONCLUSION: Operation time, bleeding volume, drainage volume and duration of blood oozing in the experimental group were significantly lower than those in the control group (P 0.05). Combined application of fibrin glue and gelatin sponge in partial liver resection can effectively reduce the operation time and reduce postoperative hemorrhage without increasing patient's cost and hospital stay.%背景:生物蛋白胶广泛应用于各种外科手术中对富含小血管创面渗血的处理.目的:通过在肝部分切除中应用组织可吸收性医用生物蛋白胶,分析其对手术时间及肝部分切除后止血效果的影响.方法:对42例肝部分切除患者随机等分为实验组和对照组.实验组在肝部分切除后,缺损处喷洒医用生物蛋白胶封闭,并以明胶海绵剪成多块小片段混合医用生物蛋白胶以覆盖断面来代替常规缝合断面.对照组仅缝合收拢肝组织缺损.结果与结论:实验组使用的生物蛋白胶可迅速附着于创面,实验组患者肝部分切除的手术时间、出血量、引流量及渗血持续时间均明显小于对照组(P 0.05).提示在肝部分切除中应用医用生物蛋白胶可以在不增加患者费用及住院时间的同时,有效降低手术时间、减少肝部分切除后出血.

  18. Simultaneous implant placement and bone regeneration around dental implants using tissue-engineered bone with fibrin glue, mesenchymal stem cells and platelet-rich plasma.

    Science.gov (United States)

    Ito, Kenji; Yamada, Yoichi; Naiki, Takahito; Ueda, Minoru

    2006-10-01

    This study was undertaken to evaluate the use of tissue-engineered bone as grafting material for alveolar augmentation with simultaneous implant placement. Twelve adult hybrid dogs were used in this study. One month after the extraction of teeth in the mandible region, bone defects on both sides of the mandible were induced using a trephine bar with a diameter of 10 mm. Dog mesenchymal stem cells (dMSCs) were obtained via iliac bone biopsy and cultured for 4 weeks before implantation. After installing the dental implants, the defects were simultaneously implanted with the following graft materials: (i) fibrin, (ii) dMSCs and fibrin (dMSCs/fibrin), (iii) dMSCs, platelet-rich plasma (PRP) and fibrin (dMSCs/PRP/fibrin) and (iv) control (defect only). The implants were assessed by histological and histomorphometric analysis, 2, 4 and 8 weeks after implantation. The implants exhibited varying degrees of bone-implant contact (BIC). The BIC was 17%, 19% and 29% (control), 20%, 22% and 25% (fibrin), 22%, 32% and 42% (dMSCs/fibrin) and 25%, 49% and 53% (dMSCs/PRP/fibrin) after 2, 4 and 8 weeks, respectively. This study suggests that tissue-engineered bone may be of sufficient quality for predictable enhancement of bone regeneration around dental implants when used simultaneous by with implant placement.

  19. Use of an autologous leucocyte and platelet-rich fibrin patch on hard-to-heal DFUs

    DEFF Research Database (Denmark)

    Löndahl, M; Tarnow, L.; Karlsmark, T;

    2015-01-01

    OBJECTIVE: Leucopatch is a leukocyte and platelet-rich fibrin patch that provides concentrated blood cells and signal substances to the surface of an ulcer. It is produced by centrifugation of the patient's own venous blood. The aim of this pilot multicentre cohort study was to evaluate effects...

  20. 生物蛋白胶封堵术治疗高位肛瘘的临床研究%Clinical application of occlusion treatment with biological fibrin glue for high peri-anal abscess

    Institute of Scientific and Technical Information of China (English)

    杨中权; 赵英武; 邓访雄; 林友彬; 莫少卫; 李昭仪

    2011-01-01

    目的 探讨生物蛋白胶封堵术治疗高位肛瘘的临床应用.方法 将我科2010年3月至2011年4月期间60例高位肛瘘,随机分为治疗组和对照组各30例.治疗组采用生物蛋白胶封堵术,对照组采用肛瘘切除术,比较两组术后创面愈合时间、创面换药疼痛、肛管压力测定.结果 治疗组术后疼痛及刨面愈合时间均比对照组低(P<0.05);复发率的差异无统计学意义(P>0.05);术后肛管静息压比较,差异有统计学意义(P<0.05).结论 生物蛋白胶封堵术治疗高位肛瘘有较好的效果,能缩短伤口愈合时间,减轻术后换药疼痛,保护肛管静息压.%Objective To discuss the clinical application of occlusion treatment with biological fibrin glue for high peri-anal abscess. Methods 60 cases with high peri-anal abscess in our hospital from Mar. 2010 - Apr. 2011 were analyzed. 60 patients were randomly divided into the treatment group and the control group (30 cases in each group). The patients in the treatment group received occlusion treatment with biological fibrin glue. The control group received anatomical fistulectomy. Results : The pain and healing time of incision in the treatment group were shortened as compared with those in the control group ( P 0. 05). There was significant difference in postoperative resting anal pressure between the groups ( P <0. 05). Conclusion : Occlusion treatment with biological fibrin glue for high peri-anal abscess shows good therapeutic effect in high peri-anal abscess, not only shorten the course of the wound's coucrescence, but also alleviate the pain of the change medication. It protected the resting anal pressure.

  1. Uso da cola de fibrina na prevenção de sangramento e hematoma pós-operatório em septoplastias Use of fibrin glue in the prevention of postoperative bleeding and hematomas after septoplaties

    Directory of Open Access Journals (Sweden)

    Silvio Caldas Neto

    2002-10-01

    Full Text Available Introdução: Entre as complicações da septoplastia, o sangramento e o hematoma septal pós-operatório estão entre as mais freqüentes1,2,3. Para prevenir tais problemas, a maioria dos cirurgiões ainda faz uso de tamponamentos nasais ou "splints", que tornam o período pós-operatório imediato extremamente desconfortável para o paciente1,2,4,5,6. Este trabalho se propõe a avaliar a eficácia e a segurança do uso da cola biológica nas septoplastias como profilaxia para essas complicações. Forma de estudo: Clínico prospectivo randomizado. Material e método: Os autores observaram 20 pacientes operados de septoplastia entre janeiro e maio de 2002 pelo Real Instituto de Otorrino e Fono, Recife, nos quais foi utilizado selante de fibrina para colar os folhetos septais. Resultados: Nenhum caso de hematoma septal ou epistaxe pós-operatórios foi observado entre os pacientes estudados. Nenhuma complicação intra ou pós-operatória foi relacionada ao uso da cola. Conclusões: A cola de fibrina mostrou ser uma substância eficaz na prevenção das complicações em questão, assim como bem tolerada pelos tecidos das cavidades nasais. Além disso, o recurso assegurou um pós-operatório muito mais tranqüilo e confortável para os pacientes do que o que se costuma observar em pacientes com tampões nasais ou "splints".Introduction: Among the complications of septoplaty, postoperative septal bleeding and hematoma are some of the most frequent. In order to avoid such problems, most surgeons still use nasal packing and/or splints that make the postoperative period extremely unpleasant. The purpose of this paper is to evaluate the efficacy and safety of fibrin glue in septoplasties for prophylaxis of these complications. Study design: Clinical prospective randomized. Material and method: We observed 20 patients who underwent septoplaties from January to May, 2002, at the Real Instituto de Otorrino e Fono, in whom we used fibrin glue, in order

  2. Fibrin glue application in breast surgery:a Meta-analysis%医用生物蛋白胶在乳腺癌手术中应用的Meta分析

    Institute of Scientific and Technical Information of China (English)

    付雷; 邢程程

    2015-01-01

    BACKGROUND:Fibrin glue has been widely used in breast surgery, but due to the limitation of cases, there are some differences in clinical effects. OBJECTIVE:To systemicaly review the clinical effect of fibrin glue in breast surgery. METHODS: We searched China Journal Ful-text Database (CNKI), VIP database, Wanfang Database for clinical randomized controled trials related to fibrin glue used in breast surgery published from March 2002 to March 2014. A Meta-analysis was done in included studies using Rev Man 5.2 statistical software. RESULTS AND CONCLUSION:A total of 11 studies, with 1 043 cases, were enroled for the Meta-analysis. Meta-analysis showed that the postoperative 1-day drainage amount and total drainage amount were significantly lower in the fibrin glue group than the control group [WMD=-85.62, 95%CI(-128.86,-42.39), P=0.000 1;WMD=-167.27, 95%CI (-210.05,-124.49),P   目的:系统评价医用生物蛋白胶在乳腺癌手术中应用的效果。  方法:计算机检索中国期刊网全文数据库(CNKI)、维普数据库、万方数据库2002年3月至2014年3月公开发表的关于在乳腺癌手术中医用生物蛋白胶应用的随机对照临床试验文献,对纳入的研究采用Rev Man 5.2统计学软件进行Meta分析。  结果与结论:Meta分析共纳入11项研究,合计1043例患者。结果显示,应用医用生物蛋白胶的实验组术后第1天引流量[WMD=-85.62,95%CI(-128.86,-42.39),P=0.0001]及术后总引流管引流量[WMD=-167.27,95%CI(-210.05,-124.49),P<0.00001]都显著少于对照组,实验组术后皮下积液率[OR=0.30,95%CI(0.21,0.44),P<0.00001]优于对照组,两组比较差异有显著性意义。提示在乳腺癌根治手术采用医用生物蛋白胶后可显著减少引流液,乳腺癌术后皮下积液的发生率明显降低。

  3. Estudo do reparo do ferimento de colon com o lado seroso da parede de jejuno, utilizando cianoacrilato e cola de fibrina Study of repair of rat's colon wound whith serosal of jejunal wall using cyanoacrylate and fibrin glue

    Directory of Open Access Journals (Sweden)

    Carlos Edmundo Rodrigues Fontes

    2006-04-01

    Full Text Available OBJETIVO: Comparar o efeito do cianoacrilato e da cola de fibrina como adesivo das bordas do ferimento de cólon reparado com o lado seroso da parede de jejuno. MÉTODO: Foram utilizados 45 ratos wistar adultos jovens, machos , com peso médio de 260 gramas procedentes do Biotério Central da Universidade Estadual de Maringá.Os animais foram submetidos a procedimento operatório sob sedação com thiopental para produção de ferimento pradonizado de 0,5 cm distante caudalmente a 2 cm do ceco que foi reparado no grupo I pelo lado seroso da parede de jejuno e sutura, no grupo II, pelo lado seroso da parede de jejuno e cola de fibrina, e o grupo III pelo lado seroso da parede de jejuno e cianoacrilato. No 4º dia de pós-operatório os animais foram submetidos a um enema opaco, para estudo o de estenose. No 7º dia de pós-operatório os animais foram submetidos a laparotomia sob sedação para estudo macroscópico da cavidade , e coleta dos segmentos que foram processados para análise microscópica. RESULTADOS: A cola de fibrina teve uma tendência de ser melhor reparadora, estimulando a proliferação de fibras de colágeno a partir da borda da ferida. CONCLUSÃO: O experimento demonstrou que o ferimento em cólon de rato pode ser reparado pelo lado seroso da parede de jejuno colado às bordas da ferida por cola de fibrina ou cianoacrilato.BACKGROUND: The present experiment aimed to compare the effect of the use of cyanoacrylate and fibrine glue as adesive on repared colon's wounds with the serosal side of jejuno's wall. METHODS: Forty five male, young adult Wister rats, weighting about 260 g , from the University of Maringá were used.After having been deeply anaesthetized (thiopental was used, the animals underwent a surgery, and a pattern 0,5 cm wound was made, 2 cm away from the cecum, in the caudal location. Group one the wound was repaired by using the jejunal serosal wall and suture.On group two the jejunal serosal wall and fibrine glue

  4. ASTIGMATIC OUTCOME & POST OPERATIVE RECOVERY IN PAT IENTS UNDERGOING PTERYGIUM EXCISION WITH CONJUNCTIVAL AUTOGRAFTS. COMPARISION BETWEEN SUTURE & AUTOLOGUS BLOOD FIBRIN

    Directory of Open Access Journals (Sweden)

    Smita Dileep

    2013-06-01

    Full Text Available ABSTRACT : AIMS: To review the effect of surgery type on the postop erative recovery & astigmatism in pterygium surgery. SETTINGS AND DESIGN: Randomized comparative clinical trial. MATERIALS AND METHODS: Forty six patients (46 eyes with primary pterygiu m were randomised to undergo pterygium surgery using eithe r autologous fibrin glue (23 eyes or 10-0 nylon sutures (23 eyes to attach the conjunctival autograft. The patients were followed up 1day, 1wk, 2wks & 6 w eeks. Outcome measures were keratometry changes & postope rative recovery.. The preoperative and postoperative keratometric measurements, evaluated using keratometer, were noted. RESULTS: Corneal astigmatism was significantly reduced in th e total group from 2.94(SD- 0.52 diopter to 1.91 (sd 0.57 diopter (p 0.05. The intensity of the postoperative watering, itchin g, were significantly lower in the autologous fibrin glue group than in the suture gro up (p<0.001. Postoperative redness was absent in 47.82% (11cases and mild in 52.18% (13 cases 2 weeks postoperatively in the autologous fibrin group while in sutured group 17.4% (4cases had mild redness and 82.6% (19 cases had moderate redness 2weeks postoperatively.

  5. A Novel Approach for Treatment of an Unusual Presentation of Radicular Cysts Using Autologous Periosteum and Platelet-Rich Fibrin in Combination with Demineralized Freeze-Dried Bone Allograft

    Directory of Open Access Journals (Sweden)

    Veena A. Patil

    2013-01-01

    Full Text Available Radicular cysts are the most common cystic lesions affecting the jaws. They are most commonly found at the apices of the involved teeth. This condition is usually asymptomatic but can result in a slow-growth tumefaction in the affected region. The following case report presents the successful treatment of radicular cysts using autologous periosteum and platelet-rich fibrin with demineralized freeze-dried bone allograft.

  6. Application of fibrin glue in conjunctival autograft surgery in rabbit pterygia model%纤维蛋白胶在兔眼翼状胬肉自体结膜瓣转移手术中的应用

    Institute of Scientific and Technical Information of China (English)

    曹亮; 宋愈; 吴莹; 孙志敏; 黄黎黎; 喻建锋

    2012-01-01

    Background Pterygia is a clinical common disease.A lot of surgical methods are developed to decrease the recurrence rate.Resent years,the application of fibrin glue is receiving more and more attention.Objective This study was to explore the effects of fibrin glue in decreasing inflammatory irritation and its mechanism. Methods Pterygia models were created in 12 clean rabbits by exsection of limbal tissue and topical administration of 1.25% diluted hydrochloric acid,and then the conjunctival autograft surgery was performed in the experimental rabbits.The conjunctival flap was sutured in the left eyes,and the conjunctival wound was closed using fibrin glue in the right eyes.The operation duration for each group was documented and compared.The irritation sign was examined under the slit lamp in all the rabbits 1 week and 4 weeks respectively.The expressions of vascular endothelial growth factor (VFGF) and basic fibroblast growth factor (bFGF) proteins in the conjunctiva tissue were detected by immunochemistry,and the expressions of VFGF mRNA and bFGF mRNA in the conjunctival tissue were determined by reverse transcription polymerase chain reaction (RT-PCR). Results The operative duration was (21.3±0.2) minutes in suture group and( 10.1 ±0.1 )minutes in the fibrin glue group with a significant difference between two groups( t =102.242,P<0.05 ).From 1 week through 4 weeks,the hyperemiain degree was obviously slight in fibrin glue group compared with suture group.Immunochemistry showed that VEGF and bFGF proteins were expressed mainly in the cytoplasm of conjunctival epithelium layer.The positive response intensity was weaker in the fibrin glue group than in suture group 1 week and 4 weeks after operation.RT-PCR revealed that the expression level of VEGF mRNA was significantly lower in fibrin glue group than in suture group,and the VEGF mRNA was gradually decreased with the time lapse ( Fgroup =174.443,P =0.000 ; Ftime =231.459,P =0.000 ).The similar outcomes were

  7. Examining the effects of platelet-rich plasma and platelet-rich fibrin on autologous full thickness skin graft survival in rats

    Directory of Open Access Journals (Sweden)

    Noorahmad Latifi

    2016-02-01

    Full Text Available Background: Graft survival has been considered the major problem in reconstructive surgery. Clinical studies have helped us to understand the role of PRP in increasing skin survival. Our goal in this study was to examine the treatment effects of platelet-rich plasma (PRP and platelet-rich fibrin (PRF on autologous full thickness skin graft survival in male rats. Methods: This experimental study was performed on 36 rats of Sprague-Dawley race with weighing approximately 250 to 300 gr on May 2015 in animal laboratory of Hazrat Fatima Hospital. After anesthesia, rats were divided into 3 groups. We injected platelet-rich plasma (PRP in the first group, platelet-rich fibrin (PRF in the second and saline in the third group after removing the skin. Microscopic analysis was performed with camera (Canon powershot SX200, Tokyo, Japan on days 7, 14, 21 and 28 after surgery. We used image analysis system (ImageJ, ver. 1.45 to examine necrosis and survival rate. Samples were studied with H&E staining on day 28 microscopically for histological analysis of vascular density and angiogenesis. Results: Our findings showed the area of necrosis in animals injected with PRP on days 7 and 14, was meaningfully less than control group (P= 0.0001. There was no meaningful difference between control and PRP groups (P> 0.05. The area of necrosis in animals injected with PRF did not have any significant difference with control group from beginning to 21st day (P< 0.0001. there was no meaningful difference in vascular density between control and PRP group, whereas in animals injected with PRF the vascular density was significantly less than control group (P= 0.002. Conclusion: According to our results in this study, we can conclude that using autologous PRP can enhance the process of healing soft tissue injury and be affective at increasing graft survival. This method is suggested to be conducted for patients highly at risk of graft loss and also for those who are in need of

  8. A fibrin-related line of research and theoretical possibilities for the use of fibrin glue as a temporary basal membrane in non-perforated corneal ulcers and in photorefractive keratectomy (PRK-operated corneas Linha de pesquisa em fibrina e possibilidades teóricas de seu uso como membrana basal provisória em úlceras corneanas não perfuradas e em córneas operadas de ceratectomia fotorrefrativa (PRK

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    José Américo Bonatti

    2007-10-01

    Full Text Available PURPOSE: To report a specific line of research developed at the University of São Paulo/Brazil on fibrin glue used for sealing corneal perforations and the perspectives of use on non-perforated corneal ulcers and photorefractive keratectomy-operated corneas. METHODS: To describe fibrin glue manufacture, development of a device to test the glued perforated corneal area resistance, subsequent experimental investigations of the use of the fibrin glue in corneal perforations, reporting its efficacy, mechanical resistance experiments and histological study. Finally, the medical literature basis is searched to propose studies on the use of fibrin as a temporary basal membrane on non-perforated corneal surfaces like non-infectious corneal ulcers and on post-photorefractive keratectomy corneal surfaces. RESULTS: The development of fibrin glue, the device for resistance experiments, the efficacy, resistance and histological studies on fibrin glue used for sealing corneal perforations are reported as well as the scientific literature basis for the proposed studies on the use of fibrin as a temporary basal membrane on non-perforated corneas like non-perforated corneal ulcers and photorefractive keratectomy corneal surfaces. CONCLUSION: A specific line of research was reported on fibrin glue to seal corneal perforations at the University of São Paulo/Brazil and the theoretical perspectives for the use of fibrin in non-perforated corneal ulcers and on photorefractive keratectomy-operated corneas in an attempt to reduce corneal haze.OBJETIVO: Relatar uma linha de pesquisa desenvolvida no Brasil (Universidade de São Paulo em cola de fibrina para tamponamento de perfuração corneana e a perspectiva de seu uso em úlceras corneanas não perfuradas e em córneas operadas de ceratectomia fotorrefrativa. MÉTODOS: Relatar a fabricação da cola de fibrina, desenvolvimento de instrumentação para ensaio de resistência da área colada, estudo experimental da

  9. The efficacy of the application of fibrin glue in the surgery for pterygium%翼状胬肉术中应用纤维蛋白胶的效果

    Institute of Scientific and Technical Information of China (English)

    杜君; 陶方方; 雷方

    2015-01-01

    目的 评价各级翼状胬肉切除联合自体结膜移植术中应用纤维蛋白胶的效果.方法 前瞻性研究.2013年4月至2013年10月在我院行原发性翼状胬肉切除联合自体结膜移植术的48例(48眼),术中应用纤维蛋白胶术前根据翼状胬肉的透明性及肥厚程度分为1~3级.评价各级术后反应、胬肉复发情况和视力.结果 术后反应有移植物移位1眼,移植物水肿4眼,移植物充血5眼.并发病的发生组间差异无统计学意义(P<0.05).术后6个月,1、2、3级组分别有0、3和1眼复发,组间差异无统计学意义(x2 =2.338,P=0.311).术后1年,1、2、3级组分别有0、3和5眼复发,组间差异有统计学意义(x2=6.236,P=0.044);各组术后视力均较术前提高,术后1周视力与术前相比差异有统计学意义(P<0.05).结论 翼状胬肉切除联合自体结膜移植术术中使用纤维蛋白胶安全、有效.%Objective To evaluate the effect of the application of fibrin glue in the surgery of all grades pterygium excision combined with conjunctival autograft transplantation.Methods Forty-eight eyes of 48 cases with primary pterygium in our department were prospectively studied.They were all treated with pterygium excision combined with conjunctival autograft transplantation surgery and were given the fibrin glue during the surgery.The patients were divided into three groups according to the transparency and pachytic degree of the pterygium.The postoperative complications,recurrence rate and visual acuity were evaluated after the surgery.Results There were 1 eye of autograft dislocation,4 eyes of edema and 5 eyes of hyperemia.There was no statistically significant difference in complication incidence rate among groups (P < 0.05).There were 0 eye,3 eyes and 1 eye recurred in three groups at the 6 months after the surgery respectively and the differences among groups were not statistically significant (x2 =2.338;P =0.311).There were 0 eye,3 eyes and 5 eyes

  10. Application of Fibrin Glue in Preventing Postoperative Complications of Colorectal Endoscopic Submucosal Dissection%纤维蛋白胶在预防结直肠内镜黏膜下剥离术后并发症的应用

    Institute of Scientific and Technical Information of China (English)

    魏真真; 李楠; 朱超慧; 翟俊山; 孟晓娇; 孙亚梅

    2015-01-01

    Objective To investigate the effect of fibrin glue on preventing postoperative complications of colorectal submucosal dissection operated by endoscopic.Methods The 53 patients with ESD were divided into two groups randomly. In treatment group(FG group),27 cases of ulcer trauma after ESD surgery were treated with APC and titanium clip, followed by fibrin glue(FG). In control group,26 cases of ulcer trauma were treated by conventional APC and titanium clip. One week after surgery, patients of two groups were observed by the incidence of fever, abdominal pain,bleeding,perforation of the complications.Results (1)Fever and abdominal pain in FG group were 2/3 cases,compared with 8/13 in the control group,there were statistically significant differences(P0.05),compared with the control group. (3)The hospitalization time of FG group and control group were(5.62±0.79)and(6.30±1.12)(P<0.05),respectively.Conclusion Fibrin glue(FG)not only can effectively reduce the incidence of fever and abdominal pain after endoscopic submucosal dissection,but also can shorten the time staying in hospital.%目的:探讨纤维蛋白胶在预防结直肠内镜黏膜下剥离术后并发症的作用及优势。方法53例符合ESD手术适应证的患者,随机分为两组,治疗组(FG组):27例ESD手术后的溃疡创面,APC及钛夹治疗基础上喷洒适量的FG予以封闭;对照组:26例ESD手术后的溃疡创面,常规APC及钛夹处理。在术后1周时间内,观察两组患者发热、腹痛、出血、穿孔等并发症的发生情况。结果(1)术后FG组发热/腹痛分别为2/3例,与对照组的8/13例比较,差异均具有统计学意义(P<0.05)。(2)术后FG组无ESD术后迟发性出血、穿孔的发生,但与对照组的3,1例比较,差异无统计学意义(P>0.05)。(3)FG组和对照组的住院时间分别为(5.62±0.79)d/(6.30±1.12) d(P<0.05)。结论纤维蛋白胶不但有效降低结直肠内镜

  11. Anastomose do nervo facial de coelhos com cola de fibrina: estudo da velocidade de condução nervosa Rabbit facial nerve anastomosis with fibrin glue: nerve conduction velocity evaluation

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    Francisco Aurelio Lucchesi Sandrini

    2007-04-01

    Full Text Available OBJETIVO: Este estudo tem o objetivo de avaliar através da velocidade de condução nervosa com eletrodos de superfície a utilização da cola de fibrina na anastomose nervosa. MÉTODOS: Neste experimento, foram avaliadas as diferenças entre as velocidades de condução nervosa pré e pós-operatória do nervo facial esquerdo de 12 coelhos. Foi verificada a existência de correlação entre a velocidade de condução nervosa e o número de axônios regenerados no pós-operatório. Os nervos transeccionados foram unidos com cola de fibrina. O potencial de ação motora foi obtido com o uso de eletrodos de superfície. O eletrodo de estimulação foi colocado imediatamente à frente do pavilhão auditivo (tronco do nervo facial e o eletrodo de gravação foi colocado no músculo quadrado do lábio inferior. RESULTADOS: A média normal da velocidade de condução nervosa foi de 36,53 m/seg. Ao final do período, a velocidade de condução nervosa atingiu um valor de aproximadamente 81% do valor normal. Não foi observada correlação significativa entre a velocidade de condução nervosa pós-operatória e o número de axônios regenerados (p=0,146. CONCLUSÃO: A anastomose com cola de fibrina pode ser utilizada para anastomose nervosa no modelo animal e nervo estudados.AIM: The aim of this study was to evaluate the use of fibrin glue on nerve anastomosis, and study conduction velocity obtained by surface electrodes. METHODS: In this experimental model we evaluated nerve conduction velocity differences in the preoperative and postoperative periods, for the left facial nerve of 12 rabbits. Then, we evaluated whether there were correlations between conduction velocity and the number of postoperative regenerated axons. The sectioned nerves were anastomosed with fibrin glue. The muscle action potentials were obtained from surface electrodes. The stimulation electrode was placed immediately before the ear pinna (facial nerve trunk and the recording

  12. Comparative study between fibrin glue and platelet rich plasma in dogs skin grafts Estudo comparativo entre a cola de fibrina e o plasma rico em plaquetas em enxertos cutâneos em cães

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    Larissa Correa Hermeto

    2012-11-01

    Full Text Available PURPOSE: Compare fibrin glue (Tissucol® and platelet-rich plasma in full-thickness mesh skin grafts in dogs. METHODS: Eighteen dogs were used, divided into two groups: fibrin glue (FG and platelet-rich plasma (PRP. In all the animals, a full-thickness 3x3 cm mesh skin graft was implanted. In the left limb, the biomaterial was place between the graft and the receptor bed, according to the group, while the right limb served as the control group. All the animals were evaluated clinically every 48 hours until the 14th day, using the variables of exudation, coloration, edema and cosmetic appearance. Three animals were evaluated histologically, on the third, seventh and tenth postoperative days, using the variables of fibroblasts, collagen, granulation tissue, microscopic integration-adherence and acute inflammation. RESULTS: Clinical evaluations showed that the group CF showed better scores for all variables compared to PRP group. On the histological evaluations PRP group had a higher presence of fibroblasts in the seventh and fourteenth days. CONCLUSION: The fibrin glue group was clinically superior to the platelet-rich group when used on full-thickness skin grafts in dogs.OBJETIVO: Comparar a cola de fibrina (Tissucol® e o plasma rico em plaquetas em enxertos cutâneos de espessura completa em malha em cães. MÉTODOS: Foram utilizados 18 cães, distribuídos em dois grupos, cola de fibrina (CF e plasma rico em plaquetas (PRP. Em todos os animais foi realizado um enxerto cutâneo de 3x3 cm, em malha de espessura completa. No membro esquerdo foi colocado o biomaterial entre o enxerto e o leito receptor, cada qual em seu grupo, o membro direito serviu como grupo controle. Todos os animais foram avaliados clinicamente a cada 48 horas até o décimo quarto dia, através das variáveis: exsudação, coloração, edema e aspecto cosmético; histologicamente em três animais, no terceiro, sétimo e décimo quarto dia de pós-operatório através das

  13. [The use of tissue glue and its effect on hospital cost in patients undergoing pulmonary surgery].

    Science.gov (United States)

    Gündoğdu, Ahmet Gökhan; Yazicioğlu, Alkin; Kara, Murat; Kanbak, Meral; Doğan, Riza

    2006-01-01

    Prolonged air leak following pulmonary resections is an important cause of morbidity and increased hospital costs. We compared 19 homologous/autologous tissue glue (fibrin glue) applied patients (FG group), 12 beriplast-P applied patients (beriplast group) and 27 control patients with respect to prolonged air leak, chest tube removal time and hospital costs. The mean ages for FG group (19 patients), beriplast group (12 patients) and control group (27 patients) were 48.5 +/- 14, 50.5 +/- 6.8 and 55 +/- 12.9 respectively. The groups were comparable with respect to age (p= 0.210), sex (p= 0.287) and the surgical procedure performed (p= 0.289). The incidence of prolonged air leak in FG group, beriplast group and the control group was 48%, 50% and 63%, respectively (p= 0.533). The mean chest tube removal time in FG group, beriplast group and the control group was 10.7 +/- 8.7, 9 +/- 4.1 and 8 +/- 3.1 days, respectively (p= 0.282). Mean hospital costs in FG group, beriplast group and the control group were 4633 +/- 3272 YTL, 4611 +/- 1583 YTL and 4015 +/- 911 YTL, respectively (p= 0.547). Fibrin glue had no effect on the incidence of prolonged air leak, chest tube removal time and hospital costs.

  14. 生物蛋白胶在未足月胎膜早破中的应用%Application of Fibrin Glue (FG) in Preterm Premature Rupture of Membranes

    Institute of Scientific and Technical Information of China (English)

    陈红; 杨军; 唐瑶; 王涛; 廖晓焰; 常林利; 蔡一玲; 李萍

    2009-01-01

    目的:探讨生物蛋白胶封堵宫颈治疗未足月胎膜早破延长孕周的可行性和安全性.方法:收集我院2006年6月至2007年12月妊娠28~32周胎膜早破患者26例分成两组,治疗组13例采用生物蛋白胶封堵宫颈,对照组13例采用期待疗法,比较两组的延长孕周时间及围生儿结局.结果:治疗组无一例发生宫内感染和过敏反应.治疗组总有效率为61.5%,明显高于对照组(15.4%),P<0.05;治疗组较对照组延长孕周时间更长,新生儿死亡率、围生儿并发症发生率更低,两组比较差异有统计学意义(P<0.05).结论:生物蛋白胶应用于未足月胎膜早破可有效延长孕周时间,并且使用安全,无副反应.%Objective:To probe into the feasibility and safety of extending the pregnancy period for pregnant women with preterm premature rupture of membranes(PPROM) by fibrin glue (FG). Methods:An investigation was made of 26 cases of PPROM from Jun 2006 to December 2007 in our Hospital. These 26 PPROM women were divided into two groups: study group treated by FG (13 cases), and expectant group without FG treatment (13 cases) as control. The extension of pregnant period and perinatal result were investigated. Results:There was no intraunterine infection and allergic response in study group. The total effective rate in FG group was 61.5%, higher than control group (15.4%) ( P<0.05); The FG group had a longer pregnant period,lower neonatal death and perinatal complication, when compare to control group ( P < 0.05). Conclusions: Fibrin glue can appropriately extend the pregnant period of women with PPROM.

  15. Nerve growth factor with fibrin glue in end-to-side nerve repair in rats Fator de crescimento nervoso em cola de fibrina no reparo término-lateral de nervos em ratos

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    Daniel Nunes e Silva

    2012-04-01

    Full Text Available PURPOSE: To determine the effects of end-to-side nerve repair performed only with fibrin glue containing nerve growth in rats. METHODS: Seventy two Wistar rats were divided into six equal groups: group A was not submitted to nerve section; group B was submitted to nerve fibular section only. The others groups had the nerve fibular sectioned and then repaired in the lateral surface of an intact tibial nerve, with different procedures: group C: ETS with sutures; group D: ETS with sutures and NGF; group E: ETS with FG only; group F: ETS with FG containing NGF. The motor function was accompanied and the tibial muscle mass, the number and diameter of muscular fibers and regenerated axons were measured. RESULTS: All the analyzed variables did not show any differences among the four operated groups (p>0.05, which were statistically superior to group B (p0.05. CONCLUSION: The end-to-side nerve repair presented the same recovery pattern, independent from the repair used, showing that the addition of nerve growth factor in fibrin glue was not enough for the results potentiating.OBJETIVO: Determinar os efeitos do reparo nervoso término-lateral realizado apenas com cola de fibrina contendo fator de crescimento nervoso em ratos. MÉTODOS: Setenta e dois ratos Wistar foram distribuídos em seis grupos: A - não submetido à secção nervosa; B - secção do nervo fibular (sem reparo; Os outros grupos tiveram o nervo fibular seccionado e então reparado na superfície lateral do nervo tibial intacto, com diferentes procedimentos: C - RNTL com suturas; D - RNTL com suturas e FCN; E - RNTL apenas com CF; F - RNTL com CF contendo FCN. A função motora foi acompanhada e a massa do músculo tibial, o número e o diâmetro das fibras musculares e axônios regenerados foram medidos. RESULTADOS: Não houve diferença entre as variáveis avaliadas nos quatro grupos operados (p>0,05, os quais foram superiores ao grupo B (p0,05. CONCLUSÕES: O reparo nervoso t

  16. Teknik Lem Fibrin Otologus pada Cangkok Konjungtiva Bulbi Mata Kelinci

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    Sutarya Enus

    2009-12-01

    Full Text Available Commercial Fibrin Glue (CFG as a biomaterial adhesive from fresh frozen plasma donor and bovine has been tested having various superiorities compared to suturing in bulbar conjunctiva grafting of pterygium surgery. Due to CFG has not been available in Indonesia led the author to make an Autologous Fibrin Glue (AFG directly from patient's own blood. The study aim was to investigate the effectiveness of AFG attachment. This study was an animal experimental, conducted in 24 New Zealand White rabbits divided into AFG group and suturing group of 12 rabbits each, held in Laboratory of Bio Farma, Bandung, from September to December 2006. The assessment included duration (in minute of AFG compared to suturing techniques, also at first day and one week post grafting attachments in bulbar conjunctiva grafting. The statistical analysis used Mann-Whitney and Wilcoxon rank test. In time duration of surgical treatment, AFG technique was significantly shorter than suturing technique (p=0.0001, while first day and one week post grafting AFG attachment showed significantly greater than those of suturing attachment (p=0.0001. In the meantime, first day and one week post grafting AFG attachment was steady state, whereas in suturing technique was different (p=0.0174. In conclusion, AFG technique is more effective than suturing technique in bulbar conjunctiva grafting because of shorter surgical duration, excellent and stable graft attachment.

  17. 骨形态发生蛋白复合纤维蛋白黏合剂临床应用16例%Clinical Application of Composite of Bone Morphogenetic Protein and Fibrin Glue in 16 Cases

    Institute of Scientific and Technical Information of China (English)

    陈剑飞; 姚伦龙; 林坚平; 吴多能

    1999-01-01

    目的用纤维蛋白黏合剂(fibrin glue,FG)作为骨形态发生蛋白(BMP)的缓释放载体,以增强诱导成骨效果.方法粗制bBMP 200 mg,纤维蛋白原1.0 g,凝血酶100 U,混合溶解液20 ml组成复合物(bBMP/FG);临床应用于股骨干骨折加压钢板内固定16例,并进行bBMP/FG的超微结构、X线片观察及4种病毒的安全监控.结果bBMP/FG结构致密厚实;术后1个月出现新骨,3个月形成"生物接骨板"效应;16例均未发现病毒感染.结论FG是BMP理想的缓释放载体,BMP/FG可形成"生物接骨板"效应,而且其病毒感染可以监控.

  18. 生物蛋白胶宫颈封堵术在未足月胎膜早破中的临床应用%Application of biological fibrin glue closure in pregnant women with preterm premature rupture of membrane

    Institute of Scientific and Technical Information of China (English)

    李小瑜; 傅斌; 黎月仪

    2014-01-01

    Objective To investigate the effectiveness of biological fibrin glue closure in pregnant women with preterm premature rupture of membrane. Methods Twenty-eight cases of pregnant women with preterm prema-ture rupture of membrane from March 2012 to December 2013 in our hospital were selected and divided into three groups randomly. All three groups were treated with biological fibrin glue closure via the cervical intubation with the monitoring of ultrasound (Group A, 10 cases), endoscopy (Group B, 8 cases) and amnioscopy (Group C, 10 cases). To-colytics were applied as following in all groups. And then the effectiveness and differences in delivery were compared. Results In Group A, the cure rate, effective rate and inefficiency rate were 0.0%(0 case), 20.0%(2 cases) and 80.0%(8 cases) respectively, with 2 cases of natural childbirth, 3 cases of operative vaginal delivery and 5 cases of cesarean section, and the fetal survival rate was 60.0%. In Group B, the cure rate, effective rate and inefficiency rate were 25.0%(2 cases), 50.0%(4 cases) and 25.0%(2 cases) respectively, with 1 case of natural childbirth, 1 case of opera-tive vaginal delivery and 3 cases of cesarean section, and the fetal survival rate was 83.3%. And in Group C, the cure rate, effective rate and inefficiency rate were 80.0%(8 cases), 10.0%(1 case) and 10.0%(1 case), with 8 cases of natu-ral childbirth, 1 case of operative vaginal delivery, 1 case of cesarean section, and the fetal survival rate was 100.0%. The total effective rate in group C was higher than those in group A and B (P<0.05). Meanwhile, compared with other groups, Group C had a longer pregnant period, lower neonatal death rate and less perinatal compilations (P<0.05). Conclusion Biological fibrin glue closure via the cervical intubation with the monitoring of amnioscopy is effective and easy to be manipulated, which can avoid the influence of water injection on the coagulation of biological fibrin glue. Therefore, it's worthy to be

  19. Tratamento de linfocele inguinal pós-operatória com injeção de cola de fibrina: relato de caso Treatment of postoperative inguinal lymphocele with fibrin glue injection: case report

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    Priscila Nunes Boaventura

    2007-06-01

    Full Text Available O paciente desenvolveu linforragia na região inguinal direita, depois de ponte aorto-bifemoral com enxerto de dácron®. Não respondeu ao tratamento conservador com cuidados locais e compressão. Foi realizado exame de ultra-sonografia Doppler, que evidenciou grande linfocele (6,4 x 3,36 x 6,1 cm, tratada pelo esvaziamento da loja por aspiração e injeção de cola de fibrina (1,6 mL em seu interior. O paciente se recuperou sem intercorrências e sem recidiva, após 3 meses de seguimento.The patient developed lymphatic drainage in the right groin after an aortobifemoral bypass with Dacron® graft. Conservative treatment with local wound care and compression was unsuccessful. Duplex scan showed a 6.4 x 3.36 x 6.1 cm lymphocele, which was treated by aspiration and injection of fibrin glue (1.6 mL. The patient recovered uneventfully and without recurrence after a 3-month follow-up.

  20. Fixação de enxerto cutâneo em malha de espessura total com sutura ou cola de fibrina Fixation of full-thickness mesh skin using suture or fibrin glue

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    M.S.P. Amaral

    2004-06-01

    Full Text Available Avaliou-se a influência da cola de fibrina, derivada do veneno de serpente, na fixação e integração de enxerto de pele. Foram utilizados nove cães, sem raça definida, com peso médio de 15kg. Foi induzida ferida de 4×4cm de área, na face crânio-proximal dos antebraços direito e esquerdo. Um enxerto de espessura total foi colhido da região torácica e expandido por meio de expansor de pele. No membro direito o enxerto foi estabilizado no leito receptor por meio de pontos isolados simples; no esquerdo foi fixado pela aplicação de cola de fibrina e oito pontos de sutura. O sítio doador foi fechado empregando-se retalho cutâneo bipediculado. As bandagens do leito receptor foram oclusivas e não aderentes e aplicou-se pomada de neomicina com bacitracina. A troca de bandagens ocorreu diariamente até o sétimo dia de pós-operatório e, posteriormente, a cada três dias. A área de sobrevivência do enxerto foi obtida pela subtração das áreas não viáveis e total medidas com fotomicroscópio Nikon conectado a um sistema de análise de imagem KS-300 aos três, sete, 15 e 30 dias de pós-operatório. Para a avaliação microscópica, a área do enxerto foi colhida em três animais aos sete, 15 e 30 dias de pós-operatório. Não houve diferença entre momentos de avaliação e técnicas de fixação quanto à área de enxerto viável. Os enxertos fixados com cola apresentaram estágio de reparação mais avançado em todos os momentos. Concluiu-se que a cola de fibrina derivada do veneno de serpente tem moderado poder adesivo e, pela análise microscópica, favorece a integração do enxerto cutâneo em malha de espessura total.The purpose of this study was to evaluate the efficiency of fibrin glue, derived from snake venom, on fixation and integration of skin graft. Nine crossbred dogs, with average weight of 15kg were used. Wounds measuring 4×4 cm were induced at the cranioproximal aspect of the right and left forelimb. Full

  1. Fibrin glue application in conjunction with tetracycline hydrochloride root conditioning and semilunar coronally repositioned flap in the treatment of gingival recession.

    Science.gov (United States)

    George, Joann Pauline; Prabhuji, M L V; Shaeesta, K B; Lazarus, Flemingson

    2011-01-01

    The purpose of this case report is to present the results of Fibrin Adhesive System (FAS) application, a topical biological tissue adhesive in the treatment of maxillary buccal recessions. A 40-year-old male patient presented with a pair of class I buccal recession defects on maxillary cuspids. Clinical parameters were recorded at baseline, 1 month, and 3 months. Semilunar coronally repositioned flap (Tarnow's technique), root debridement, root conditioning with tetracycline hydrochloride solution and one of the defect was treated with FAS application. Significant recession, height reduction, and attachment gain were observed with the FAS application defect site. About 50% of root coverage was noted in the FAS-treated defect and almost no root coverage in the defect site with no FAS application. Semilunar coronally repositioned flap with FAS application is an effective procedure for root coverage in anterior teeth. The addition of FAS improves the amount of root coverage, especially in relatively shallow defects.

  2. 多孔羟基磷灰石与富血小板血浆和纤维蛋白胶复合修复骨缺损★%Compound of porous hydroxyapatite, platelet-rich plasma and fibrin glue in the treatment of bone defects

    Institute of Scientific and Technical Information of China (English)

    刘东光; 韦正超; 蔡道章; 郑军; 陈环球; 赖欢乐

    2013-01-01

      BACKGROUND: Autogenous bone graft is the best way to treat bone defects, but its limited sources and donor site complications restrain its clinical application. Therefore, to develop a substitute material has been a hotspot in the orthopedics. OBJECTIVE: To investigate the feasibility and effectiveness of the compound of coral ine hydroxyapatite porous, platelet-rich plasma and fibrin glue acting as a bone substitute material for repair of bone defects. METHODS: 1.5 cm radial segments from the bilateral forearms of New Zealand white rabbits were removed to prepare bone defect models. Then, bone defect models were randomly divided into a compound group (coral ine hydroxyapatite porous, platelet-rich plasma and fibrin glue), control group treated with autogenous bone graft, and blank group without implantation. RESULTS AND CONCLUSION: (1) X-ray observation: Bone defects were repaired completely in the compound group til the end of postoperative week 12, appearing with complete plasticity. The healing process in the compound group was synchronized with that in the control group. However, there were no changes in bone defects of the blank group. (2) Histopathological examination: Bone repair basical y completed in the compound and control groups at week 12 after operation, presenting with mature lamel ar bone and Haversian canals. In the blank group, only a great amount of fibroblasts proliferated, but no bone formed. (3) Biomechanical analysis: The maximum torque and torsional stiffness of the compound group were better than those of the control group at 2 weeks postoperation (P < 0.05). However, there was no significant difference between the maximum torque and torsional stiffness of the compound and control groups. These results demonstrate that the compound of coral ine hydroxyapatite porous, platelet-rich plasma and fibrin glue acting as a bone substitute is superior to autologous bone in the early repair of bone defects, which is beneficial to bone healing

  3. TISSEEL纤维蛋白胶在翼状胬肉切除联合自体结膜角膜缘干细胞移植的应用%Application of TISSEEL fibrin glue in pterygium excision combined with autogrifts conjunctival limbal stem cell

    Institute of Scientific and Technical Information of China (English)

    牟洁

    2016-01-01

    Objective:the clinical applications TISSEEL fibrin glue (Tisseel fibrin glue)in pterygium excision combined with autogrifts conjunctival limbal stem cell.Methods:In Oct 2014 to July 2015,the nine cases (9 eyes)had nasal pterygium were collected for clinical applications in our depart-ment,all using TISSEEL fibrin glue,underwent pterygium excision combined with autogrifts conjunctival limbal stem cell.Postoperative five patients were wear soft contact lens fort 3-5 days,four cases no wear.Results:Patients were followed up 6 mo,8 cases had no recurrence,1case recurrence,re-currence rate 11.1%.Conclusion:TISSEEL fibrin glue is applied pterygium excision is safe and effective and convenient,and can shorten the operation time,less discomfort for the patient,less postoperative inflammation,rapid recovery,low recurrence rate.%目的:探讨TISSEEL纤维蛋白胶(Tisseel fibrin glue)在翼状胬肉切除联合自体结膜角膜缘干细胞移植术的应用情况。方法:收集(2014-04/2015-03)我院眼科门诊翼状胬肉患者9例共9眼,全部使用TISSEEL纤维蛋白胶,均行翼状胬肉切除联合自体结膜角膜缘干细胞移植术,其中5例术后配戴治疗型软性角膜接触镜3-5天,4例无戴。结果:观察手术时间、术后结膜植片贴附情况,球结膜充血及结膜下出血,以及患者术眼不适症状。术后随访6个月,8例无复发。1例复发。结论:TISSEEL纤维蛋白胶应用于翼状胬肉切除是安全有效又方便的,并可缩短手术时间,减轻术后眼部不适感,术后炎症反应轻,恢复快,复发率低。

  4. Clinical Observation of Applying Fibrin Glue in the Pterygium Excisions with Sutureless Amniotic Membrane Transplantation%纤维蛋白胶在翼状胬肉切除联合无缝线羊膜移植术中应用的临床观察

    Institute of Scientific and Technical Information of China (English)

    邸新; 姚涛; 赵宇丹; 何伟

    2010-01-01

    目的 观察与探讨纤维蛋白胶在翼状胬肉切除联合羊膜移植术中应用的方式方法和临床效果.方法 筛选原发性翼状胬肉患者21人(26只眼),行胬肉切除联合羊膜移植术.所有患者随机分为无缝线组及缝线组:无缝线组11人(13只眼),术中将胬肉组织切除后,采用纤维蛋白胶黏合固定羊膜植片;缝线组10人(13只眼),以10-0尼龙缝线间断缝合固定羊膜植片.术后随访6个月,记录手术时间、术后症状和体征.并应用SPSS统计软件对两组结果进行分析.结果 无缝线组手术时间(13.6±3.5)min明显短于缝线组(22.3±5.2)rain(P<0.01).术后2周内(拆线前),无缝线组术眼疼痛、异物感和流泪等局部刺激症状均显著轻于缝线组(P<0.01).两组术后均未发生植片移位并发症.无缝线组术后羊膜植片贴附紧密,均于术后1周内迅速上皮化.缝线组术后有5只眼(38.5%)发生羊膜下积液,羊膜上皮化较无缝线组略迟,于术后8~ 10d完全上皮化.无缝线组羊膜愈合良好,局部无明显瘢痕发生;缝线组均出现不同程度的缝线刺激性瘢痕.术后6个月随访期内两组均未见胬肉复发.结论 在翼状胬肉切除联合羊膜移植术中使用纤维蛋白胶可替代手术缝线,使用方便,并明显缩短手术时间,减轻术后刺激症状,并利于羊膜愈合、减轻局部瘢痕,达到更好的美容目的 .%Objective To observe and investigate the clinical efficacy of fibrin glue for attaching human amniotic membrane after primary pterygium excision. Methods Twenty-one patients (26 eyes) with primary pterygium underwent pterygium excision with amniotic membrane transplantation.Patients were randomized to two groups: fibrin glue group and suture group.In fibrin glue group, 11 patients (13 eyes) underwent pterygium excision, and the bare sclera was covered with an amniotic membrane using fibrin glue for graft adherence; while in suture group,10 patients (13 eyes), after

  5. Local application of fibrin glue carrying gatifloxacin%生物蛋白胶载体复合加替沙星缓释剂局部应用的实验研究

    Institute of Scientific and Technical Information of China (English)

    张守平; 曹斌; 修晓光; 吴滨滨

    2011-01-01

    背景:生物蛋白胶作为生物性抗生素载体,已在临床工作中得到广泛的应用,但生物蛋白胶与第四代氟喹诺酮类广谱抗菌药物加替沙星联合应用的实验研究鲜有报道.目的:拟建立动物局部感染模型,观察生物蛋白胶-加替沙星缓释剂对感染的预防作用及二者联合应用的安全性.方法:新西兰大白兔随机分成3组,均建立局部软组织感染模型,实验组局部放置生物蛋白胶+加替沙星,对照组局部注射加替沙星,空白组局部注射生理盐水.3组动物分别于术前和术后第3,7,14天耳缘静脉采血,进行白细胞计数检查和实验组全血药物浓度测定,同时,分别于术后第3,7,14天各切取切口旁小块肌肉组织,进行组织学检查以及实验组的局部药物浓度测定.结果与结论:空白组动物在术后2周内均出现切口化脓,而对照组和实验组未见切口化脓;白细胞计数结果显示在术后各时间点实验组白细胞计数均低于空白组(P < 0.01),而对照组在术后第7和14天白细胞计数显著高于实验组,低于空白组(P < 0.01或P < 0.05);实验组血浆和距切口约1.5 cm处肌肉组织加替沙星的浓度均在给药后3,7,14 d逐渐降低(P < 0.05);组织学检查结果显示空白组动物肌肉组织中充满大量的炎性细胞,对照组第3,7天时,肌肉组织中有少量炎性细胞,14 d时消失,实验组第3天时炎性细胞较少,第7,14天时,无炎性细胞浸润.结果表明,生物蛋白胶是抗感染药物释放系统的良好生物载体,与加替沙星联合应用,可以减少出血,降低感染发生概率.%BACKGROUND: Fibrin glue as a biological carrier of antibiotics, has been widely used in clinical work, but experimental study on fibrin glue in combination with the fourth generation of fluoroquinolone broad-spectrum antibiotic gatifloxacin has been rarely reported.OBJECTIVE: To evaluate the effects of fibrin tissue adhesive and gatifloxacin on

  6. 医用生物蛋白胶对LEEP治疗宫颈上皮内瘤变创口愈合的影响%Effects of Fibrin Glue on Wound Healing after the LEEP Treatment of Cervical Intraepithelial Neoplasis

    Institute of Scientific and Technical Information of China (English)

    虞如芬; 陈金春; 肖苹妹; 郝力

    2011-01-01

    Objective To study the effect of fibrin glue on the wound - healing after the loop electrosurgical exicision procedure, LEEP treatment of cervical intraepithelial neoplasia. Methods AH 128 patients with cervical intraepithelial neoplasia suit for diagnosis and surgical conditions were chosen. Then those patients were divided into two groups:the treatment group with 82 patients and the control group with 46 patients. Both groups adopted LEEP treatment. Wound dressing after surgery was different. One group was fibrin glue and sterile gauze for another. The incidence of complications (wound infection, granulation, scab period, cervical stenosis) ,the vaginal bleeding time and loss,the wound healing time,the treatment of effects and rate of relapse's after two years were observed. And statistical analysis were carried out. Results The complication rates for the two groups were 2.44% and 15.22% . The vaginal bleeding time≤2 weeks, >2 weeks but≤3 weeks, > 3 weeks but ≤ 5weeks, the rates ≤ 5 weeks were 78. 05% and 23. 91% , 19. 51% and 50. 00% , 2. 44% and 17. 39% ,0. 00% and 8. 70% respectively. The vaginal bleeding loss≥20ml,21 ~ 49ml, ≤50ml and the related rates were 78. 05% and 34.78% ,21.95% and 52.17% ,0.00% and 13.04% respectively. The average wound - healing time was 12. 1 ± 5. 3 days and 18. 4 ± 4. 8 days. The cure rate after two years was 98. 78% and 86. 96% respectively. There was significant difference between the two groups (P<0.05). Conclusion Fibrin glue can promote the wound -healing after LEEP treatment of cervical intraepithelial neoplasia,reduce the complications and improve the cure rate.%目的 研究医用生物蛋白胶对子宫颈电热圈环切术(loop electrosurgical excision procedure,LEEP)治疗宫颈上皮内瘤变,术后创口愈合的影响.方法 选择符合诊断和手术条件的宫颈上皮内瘤变患者128例,分治疗组82例和对照组46例,均用LEEP治疗,术后创口分别敷用医用生物蛋白胶和无菌纱

  7. Application of fibrin glue with bandage contact lens in recurrent pterygium surgery%纤维蛋白胶联合绷带型角膜接触镜在复发性翼状胬肉手术中应用

    Institute of Scientific and Technical Information of China (English)

    蔡卫国; 王秀春; 陈伟芳; 郑通美

    2016-01-01

    Objective To explore the clinical efficacy of fibrin glue with bandage contact lens for recurrent pteryg -ium surgery.Methods The study included 63 eyes of 60 recurrent pterygium patients who underwent pterygium excision combined with limbal stem cell conjunctival autograft transplantation from 2013 to 2015 in our hospital .Patients were ran-domly divided into experimental group (A) where bandage contact lens and fibrin glue were used and control group (B) where bandage contact lens and suture method were used .Surgery duration , ocular symptoms , healing time , recurrence rate and adverse reactions were evaluated .Results Our results showed that surgery duration , ocular symptoms , healing time, recurrence rate and adverse reaction are all better in patients who received fibrin glue and bandage contact lens . Conclusions Fibrin glue with bandage contact lens is a safe and effective treatment for recurrent pterygium .%目的探讨纤维蛋白胶联合绷带型角膜接触镜应用于复发性翼状胬肉手术的临床效果。方法对2013~2015年期间我院复发性翼状胬肉60例(63只眼)行翼状胬肉切除联合带自体角膜缘干细胞的结膜移植术,随机分为试验组( A)应用纤维蛋白胶联合绷带型角膜接触镜、对照组( B)应用缝线法联合绷带型角膜接触镜,观察手术时间、术后眼部症状、愈合、复发率、不良反应,评价其临床疗效。结果2组间比较手术时间、术后眼部症状、愈合、复发率及不良反应A组均优于B组。结论对于复发性翼状胬肉,应用纤维蛋白胶联合绷带型角膜接触镜手术时间、术后症状、愈合、复发率、不良反应更优,具有广泛的临床实用价值。

  8. 蛋白胶-罂粟碱复合体促进调Q Nd:YAG激光治疗后创面愈合的观察%Using Fibrin Glue Papaverine Complex to Promote Wounds Healing after Q-Switched Laser

    Institute of Scientific and Technical Information of China (English)

    朱玉; 李栋梁; 陈志勇; 谭强; 周业松

    2011-01-01

    目的 观察蛋白胶-罂粟碱复合体对调Q Nd:YAG激光治疗后创面愈合的促进作用.方法 太田痣患者50例,随机分为研究组和对照组,每组25例.以调Q Nd:YAG激光治疗后,分别应用蛋白胶-罂粟碱复合体或凡士林纱布覆盖创面,对比观察两组患者创面疼痛评分、止血时间及愈合时间.结果 研究组在创面疼痛评分、止血时间、创面愈合时间均优于对照组.结论 蛋白胶-罂粟碱复合体对调Q Nd:YAG激光治疗后的创面具有明显的减轻疼痛,促进愈合的作用.%Objective To observe the effects of using fibrin glue papaverine complex to promote wound healing after Q-switched laser therapy.Methods Fifty patients with nevus of Ota were randomly and equally assigned to two groupa: the study group applied fibrin glue papaverine complex and the control group applied vaseline gauze,and their wounds pain, hemostatic lime and healing time were compared.Results Covering wounds with glue papaverine complex after Q-switched laser was superior to the other therapy from aspects of pain alleviation, hemostatic time and healing time.Conclusions Covering wounds with glue papaverine complex after the treatment of Q-switched laser can alleviate the pain and accelerate the wound healing.

  9. The laparoscopy splenic injury repair: the use of fibrin glue in a heparinized porcine model O reparo de lesão esplênica por laparoscopia: o uso da cola de fibrina em porcos heparinizados

    Directory of Open Access Journals (Sweden)

    Dalmer Faria Freire

    2011-06-01

    Full Text Available PURPOSE: To investigate the effectiveness of fibrin glue (laparoscopic via into promote the hemostasis of a spleen injury on a heparinized porcine model. METHODS: Eighteen Landrace porcine were submitted to laparoscopic spleen injury and randomly distributed: GHA (heparin plus adhesive, GH (heparin without adhesive and GS (Sham - without heparin or adhesive. Ten minutes before the surgical procedures a single IV dose (200UI/kg of heparin sodium was administrated only to groups GHA and GH. In the GHA, adhesive was applied after the mechanical injury and recorded the time until the polymerization and clot formation. RESULTS: No significant differences occurred among the groups (Fisher test considering the weight and surgery time. The blood amount in the abdominal cavity on GH was significantly higher in comparison to the sham group and especially with the GHA (pOBJETIVO: Investigar a eficácia da cola de fibrina (via laparoscópica na hemostasia de uma lesão no baço de porco heparinizado. MÉTODOS: Dezoito suínos Landrace foram submetidos a lesão do baço e distribuídos aleatoriamente: GHA (heparina adesivo, GH (heparina sem adesivo e GS (Sham - sem heparina ou adesivo. Dez minutos antes dos procedimentos uma dose única (200UI/kg de heparina sódica (EV foi administrada nos grupos GHA e GH. A fibrina (GHA foi aplicada após a lesão e registrado o tempo até a polimerização e formação do coágulo. RESULTADOS: Não houve diferenças significativas entre os grupos (teste de Fisher, considerando o peso e o tempo de cirurgia. A quantidade de sangue na cavidade abdominal de GH foi significativamente maior em comparação ao GS e, especialmente, com o GHA (p<0,004. Não foram observadas diferenças significativas na temperatura corporal, frequência cardíaca, débito cardíaco, pressão arterial ou pressão da artéria pulmonar durante o experimento (20 minutos. O tempo de tromboplastina parcial ativada (TTPA foi menor no GHA em relação ao

  10. Platelet-rich plasma (PRP) and Platelet-Rich Fibrin (PRF): surgical adjuvants, preparations for in situ regenerative medicine and tools for tissue engineering.

    Science.gov (United States)

    Bielecki, Tomasz; Dohan Ehrenfest, David M

    2012-06-01

    The recent developement of platelet concentrate for surgical use is an evolution of the fibrin glue technologies used since many years. The initial concept of these autologous preparations was to concentrate platelets and their growth factors in a plasma solution, and to activate it into a fibrin gel on a surgical site, in order to improve local healing. These platelet suspensions were often called Platelet-Rich Plasma (PRP) like the platelet concentrate used in transfusion medicine, but many different technologies have in fact been developed; some of them are even no more platelet suspensions, but solid fibrin-based biomaterials called Platelet-Rich Fibrin (PRF). These various technologies were tested in many different clinical fields, particularly oral and maxillofacial surgery, Ear-Nose-Throat surgery, plastic surgery, orthopaedic surgery, sports medicine, gynecologic and cardiovascular surgery and ophthalmology. This field of research unfortunately suffers from the lack of a proper accurate terminology and the associated misunderstandings, and the literature on the topic is quite contradictory. Indeed, the effects of these preparations cannot be limited to their growth factor content: these products associate many actors of healing in synergy, such as leukocytes, fibrin matrix, and circulating progenitor cells, and are in fact as complex as blood itself. If platelet concentrates were first used as surgical adjuvants for the stimulation of healing (as fibrin glues enriched with growth factors), many applications for in situ regenerative medicine and tissue engineering were developed and offer a great potential. However, the future of this field is first dependent on his coherence and scientific clarity. The objectives of this article is to introduce the main definitions, problematics and perspectives that are described in this special issue of Current Pharmaceutical Biotechnology about platelet concentrates.

  11. Fibrin sealant in general surgery. Personal experience and literary review.

    Science.gov (United States)

    Gubitosi, Adelmo; Ruggiero, Roberto; Docimo, Giovanni; Esposito, Alessandro

    2014-01-01

    In consideration of the use of fibrin glue in a general surgery department, authors analyze their last two years series. Operations on liver and biliary ducts, bowel and proctologic surgery, thyroid and breast surgery, abdominal wall hernias, fistulas and difficult wounds are considered with a literary review on fibrin sealant.

  12. 化学去细胞异体神经周围复合BMSCs生物蛋白胶复合物促周围神经缺损修复%EFFECT OF CHEMICAL EXTRACTED ACELLULAR NERVE ALLOGRAFT SUPPLEMENTING WITH BONE MARROW MESENCHYMAL STEM CELLS EMBEDDED IN FIBRIN GLUE ON FUNCTIONAL RECOVERY OF TRANSECTED SCIATIC NERVES

    Institute of Scientific and Technical Information of China (English)

    赵喆; 许文静; 卢世璧; 王玉; 彭江; 赵斌; 赵庆; 刘炎; 任志午; 詹胜峰; 张莉

    2011-01-01

    目的 将BMSCs复合在化学去细胞异体神经(chemical extracted acellular nerve allograft,CEANA)周围,观察对CEANA修复周围神经缺损效果的影响.方法 成年雄性C57小鼠21只,体重25~30 g;成年雄性Balb/c小鼠15只,体重25~30 g.取Balb/c小鼠双侧坐骨神经,制备CEANA.取C57小鼠3只,分离培养BMSCs,取5 x 106个第3代BMSCs添加到500μL生物蛋白胶制备BMSCs生物蛋白胶复合物,共培养3、7、14、21 d后,分别取其上清与PC12细胞共培养,观察对PC12细胞的影响.取成年雄性C57小鼠18只,制备小鼠左侧坐骨神经10mm缺损模型,随机分成3组(n=6),分别采用自体神经移植复合生物蛋白胶(A组)、CEANA移植复合BMSCs生物蛋白胶复合物(B组)、CEANA移植复合生物蛋白胶(C组)修复坐骨神经缺损;实验动物右侧切开暴露坐骨神经,作为正常对照.术后行大体观察;术前及术后2、4、6、8周测量小鼠坐骨神经指数(static sciatic index,SSI);术后8周取材计算术侧小腿三头肌湿重恢复率并行小腿三头肌Masson染色观察,吻合口远端神经行甲苯胺蓝染色和透射电镜观察.结果 BMSCs在生物蛋白胶内均匀分布,外观呈球形,培养3d后可见BMSCs呈多个长突起.加入BMSCs生物蛋白胶复合物共培养3、7、14、21 d的上清,PC12细胞均分化为类神经元样细胞.术后各组动物切口愈合良好.各组SSI随时间延长逐渐增加,术后4、6、8周A组SSI均高于B、C组,差异有统计学意义(P0.05).术后8周,B组小腿三头肌湿重恢复率、有髓神经纤维总数均优于C组,但较A组差,差异有统计学意义(P0.05).结论 在CEANA周围添加BMSCs生物蛋白胶复合物可提高周围神经损伤修复效果.%Objective To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) embedded in fibrin glue around chemical extracted acellular nerve allograft (CEANA) on the peripheral nerve regeneration. Methods Twenty-one adult male C57 mice (weighing 25-30 g

  13. Autologous cartilage fragments in a composite scaffold for one stage osteochondral repair in a goat model

    Directory of Open Access Journals (Sweden)

    A Marmotti

    2013-08-01

    Full Text Available We propose a culture-free approach to osteochondral repair with minced autologous cartilage fragments loaded onto a scaffold composed of a hyaluronic acid (HA-derived membrane, platelet-rich fibrin matrix (PRFM and fibrin glue. The aim of the study was to demonstrate in vitro the outgrowth of chondrocytes from cartilage fragments onto this scaffold and, in vivo, the formation of functional repair tissue in goat osteochondral defects. Two sections were considered: 1 in vitro: minced articular cartilage from goat stifle joints was loaded onto scaffolds, cultured for 1 or 2 months, and then evaluated histologically and immunohistochemically; 2 in vivo: 2 unilateral critically-sized trochlear osteochondral defects were created in 15 adult goats; defects were treated with cartilage fragments embedded in the scaffold (Group 1, with the scaffold alone (Group 2, or untreated (Group 3. Repair processes were evaluated morphologically, histologically, immunohistochemically and biomechanically at 1, 3, 6 and 12 months. We found that in vitro, chondrocytes from cartilage fragments migrated to the scaffold and, at 2 months, matrix positive for collagen type II was observed in the constructs. In vivo, morphological and histological assessment demonstrated that cartilage fragment-loaded scaffolds led to the formation of functional hyaline-like repair tissue. Repair in Group 1 was superior to that of control groups, both histologically and mechanically. Autologous cartilage fragments loaded onto an HA/PRFM/fibrin glue scaffold provided a viable cell source and allowed for an improvement of the repair process of osteochondral defects in a goat model, representing an effective alternative for one-stage repair of osteochondral lesions.

  14. Clinical application of fibrin glue for attaching limbal-conjunctival autograft after pterygium blunt dissection%翼状胬肉钝性剥离联合纤维蛋白胶固定自体角膜缘干细胞移植术的临床应用

    Institute of Scientific and Technical Information of China (English)

    韩文杰; 崔波; 宋艳玲; 徐教伦

    2012-01-01

    目的 观察翼状胬肉钝性剥离联合纤维蛋白胶固定自体角膜缘干细胞移植术治疗翼状胬肉的临床效果,评价纤维蛋白胶固定球结膜移植片的有效性和安全性.方法 对原发性鼻侧翼状胬肉患者(20只眼)行翼状胬肉钝性剥离联合纤维蛋白胶固定自体角膜缘干细胞移植术,术中钝性剥离翼状胬肉头部,并采用纤维蛋白胶固定球结膜移植片,术后随访6个月.结果 所有患者的球结膜移植片均成功固定于巩膜表面,并全部成活;平均手术时间(20.5±3.6)min;术后患者的疼痛、异物感、流泪等刺激症状轻微;随访期间所有病例均无复发.结论 翼状胬肉钝性剥离联合纤维蛋白胶固定自体角膜缘干细胞移植术是一种安全、有效、较为理想的手术方式,手术时间短,术后患者刺激症状轻微.%OBJECTIVE To observe the effect of clinical application of fibrin glue for attaching limbal-con-junctival autograft after pterygium blunt dissection,and assess the efficiency and safety of using a fibrin glue to adhere the limbal-conjunctival autograft. METHODS Twenty patients (20 eyes) with primary nasal pterygium were subjected to limbal-conjunctival autografts surgery. The pterygium was blunt dissected and the autograft was fixed to the bare sclera using the fibrin glue. The follow-up period was 6 months. RESULTS All limbal-conjunctival autografts in this group were successfully fixed and intact during the 6 months. The time of surgery was 20.5±3.6 minutes. Sbjective symptoms of pain, foreign body sensation and watering were mild. There were no cases of recurrence of the pterygium. CONCLUSIONS The method of application of fibrin glue for attaching limbal-conjunctival autograft after pterygium blunt dissection was safe and effective. This technique could reduce the time of surgery and avoid the sensation of irritation.

  15. 早期应用富血小板血浆凝胶对自体脂肪组织移植存活率的影响%Platelet-rich plasma fibrin glue in the early stage for survival rate of autogeneic fat graft

    Institute of Scientific and Technical Information of China (English)

    黎洪棉; 柳大烈; 吴涛; 赵培冉; 梁双武

    2011-01-01

    背景:自体颗粒脂肪组织填充广泛用于修复重建领域,移植后组织大量被吸收可严重影响远期效果.目的:观察早期应用富血小板血浆对自体移植脂肪组织存活率的影响.方法:取健康成年人腹部脂肪组织颗粒进行纯化,同时抽取少量静脉血,采用离心法提取自体富血小板血浆,利用纤维蛋白胶的物理特性制备含有富血小板血浆的脂肪组织复合移植物,在裸鼠背部中线两侧各分离一个腔隙,富血小板血浆组将脂肪组织颗粒-富血小板血浆凝胶随机注射入一侧腔隙深筋膜下,对侧仅注入脂肪组织颗粒作为对照组.结果与结论:移植后1个月和3个月,与对照组比较,富血小板血浆组移植脂肪局部的血管增生均较明显(P < 0.05),脂肪质量保持率均较高(P < 0.05);移植物脂肪细胞纤维坏死率均较低(P < 0.05).提示早期应用富血小板血浆凝胶可促进移植脂肪组织局部的血管再生,增加脂肪组织的质量保持率,减少脂肪移植后的纤维坏死程度.%BACKGROUND: Autogeneic fat is the ideal filling material in aesthetic and reconstructive surgery. However, the autogeneic fat graft produces serious impact on long-term effect because much fat would be absorbed after transplantation. Accordingly,difficulties in this field are how to increase the survival rate of autogeneic fat graft.OBJECTIVE: To investigate the effect of platelet-rich plasma (PRP) on the survival rate of autogeneic free grain fat grafts.METHODS: The fat was harvested from abdominal region of healthy adults and purified. At the same time, venous blood was taken from concurrent patients and PRP was prepared by centrifugation. The grafts were made using fibrin glue combined with PRP according to the physical characteristics of fibrin glue. A subdermal pocket was created in each side of the dorsal midline,the grafts which carried fat particle and PRP fibrin glue were randomly implanted below the deep fascia of

  16. Clinical evaluation of autologous platelet-rich fibrin in the treatment of multiple adjacent gingival recession defects: a 12-month study.

    Science.gov (United States)

    Tunalι, Mustafa; Özdemir, Hakan; Arabacι, Taner; Gürbüzer, Bahadir; Pikdöken, Levent; Firatli, Erhan

    2015-01-01

    Leukocyte- and platelet-rich fibrin (L-PRF) belongs to a new generation of platelet concentrates. There are limited numbers of studies focused on the use of L-PRF in gingival recession defects. This study evaluated the safety and effectiveness of using L-PRF membranes as a substitute for free connective tissue grafts (CTGs) as a treatment method for gingival recession defects. A total of 44 Miller Class I/II gingival recessions that were bilateral, adjacent, and greater than 3 mm in size were selected. Each recession site was randomly assigned to the test group (L-PRF) or the control group (CTG). After 12 months, root coverage was 76.63% and 77.36% in the L-PRF and CTG groups, respectively. It is suggested that L-PRF membrane may be an alternative graft material for treating multiple adjacent recessions greater than 3 mm in size without a requirement for additional surgery.

  17. O papel do adesivo de fibrina e da sutura na fixação do transplante de menisco preservado por ultracongelamento em coelhos The role of fibrin glue and suture on the fixation of ultra frozen preserved meniscus transplantation in rabbits

    Directory of Open Access Journals (Sweden)

    Leandro José Reckers

    2009-10-01

    Full Text Available OBJETIVO: Avaliar a capacidade do adesivo de fibrina em promover a fixação do menisco ao longo de duas, quatro e oito semanas comparando com a técnica convencional de fixação por sutura a tecidos moles. MÉTODOS: 36 meniscos mediais direitos de coelhos preservados a 73°C negativos por 30 dias foram transplantados para os animais da mesma amostra e fixados com sutura ou cola de fibrina. Após duas, quatro ou oito semanas a aparência dos meniscos e a qualidade da fixação foram verificadas macroscopicamente e avaliadas por um sistema de escores. Os achados foram submetidos a estudo estatístico de análise de variância por postos (p OBJECTIVE: To evaluate the ability of fibrin adhesive in promoting the meniscus fixation within two, four and eight weeks compared to the conventional soft-tissue suture technique. MATERIALS AND METHODS: 36 right medial menisci of rabbits preserved at negative 73° Celsius for 30 days were transplanted to animals of the same sample and fixed with soft-tissue suture or fibrin glue. After 2, 4 or 8 weeks, the appearance of the menisci and the quality of fixation were macroscopically checked and evaluated by a scoring system. The findings were subjected to the statistical study of variance analysis (p < 0.05%. RESULTS: The deep-frozen meniscus preservation maintained the integrity of the meniscus transplant, and, macroscopically, there was no significant reduction of the length of the meniscus in all post-transplant periods (p = 0.015. The menisci fixed with fibrin showed slight changes in color and surface roughness. There were no signs of rejection or infection in both groups. Suture fixation scoring was superior (p = 0.015 in all periods (80% of total fixation as compared to the setting promoted by fibrin (20% of total fixation. CONCLUSION: The homologous transplantation of the meniscus of rabbits experienced various degrees of integration to the knee according to the fixation method; the surgical soft tissues

  18. Application of fibrin glue versus suture in pterygium excision and conjunctival autograft transplantation: A meta analysis%纤维蛋白胶和缝线在翼状胬肉切除联合自体结膜移植手术中应用的Meta分析

    Institute of Scientific and Technical Information of China (English)

    胡健; 贾亮; 王丽强; 黄一飞; 王瑛; 马天驹; 孟晓丽

    2015-01-01

    Objective To observe the fibrin glue versus suture in pterygium excision and conjunctival autograft transplantation. Methods From January 1, 2000 to December 31, 2014, database including Medline, EMBASE, Cochrane Central Register of Controlled Trials, and Google Scholar Search were searched with the keywords of "pterygium", "fibrin glue" and "suture". Data were extracted from randomized controlled trials (RCTs) that comparedfibrin glue with suture in pterygium surgery with a minimum follow up for six months. Postoperative recurrence, surgical time, postoperative complications were analyzed by Revman 5.0 software.Results Five RCTs with 336 eyes were eligible in this meta-analysis. There was no significant difference between two groups in term of recurrence rate in postoperative 3 months (2.04%vs 6.25%,P=0.36), while the recurrence rate showed significant differences between two groups in postoperative 6 months (4.19%vs 11.24%,P=0.02) and 12 months (4.08%vs 12.93%,P=0.01). Thefibrin glue group was less time-consuming in surgical time (P=0.000), while no significant differences were found between two groups in term of postoperative total complications (P=0.09). All graft-fixation-related complications occurred in thefibrin glue group were healed after observation or treatment.Conclusion Fibrin glue shows less recurrence, less surgical time consuming compared with suture in pterygium excision and conjunctival autograft transplantation.%目的 采用Meta分析比较纤维蛋白胶和缝线在翼状胬肉切除联合自体结膜移植手术中的效果.方法 应用搜索词"pterygium","fibrin glue"和"suture"在数据库Medline、EMBASE、Cocharne Central Register of Controlled Trials和Google Scholar Search进行检索,检索日期范围2000年1月1日- 2014年12月31日.两位独立作者对文献进行筛选、评估,对符合要求的随机对照试验文献提取数据.主要观察指标为术后复发率、手术时间、术后并发症.结果 最终有5

  19. 自体富血小板纤维蛋白用于延迟牙再植1例%Using platelet-rich fibrin as an autologous media for delayed teeth replantation:a cases report

    Institute of Scientific and Technical Information of China (English)

    王伟; 黄超; 刘艳丽; 赵寅华; 张旻; 陈永进

    2016-01-01

    PRF ( platelet rich fibrin) is a kind of second generation platelet concentrate with a three dimensional reticular structure which can regulate inflammation, accelerate tissue healing, and release a variety of growth factors slowly and continuously. However, no clinical study has used PRF for the treatment of avulsed tooth. In this study, a delayed and dry-reserved avulsion tooth was replanted with autologous PRF. 12-month follow-up showed that the tooth recovered well, the peridental membrane was healed, no root resorption occurred, and the height of alveolar bone was restored.%富血小板纤维蛋白( PRF)是一种立体三维网状结构的第二代血小板浓缩物,它能够调节炎症,促进组织愈合。并且能够缓慢持续释放多种生长因子。大量文献报道PRF可以促进口腔软硬组织的愈合,但尚未将其应用于临床牙再植的治疗中。本文联合自体PRF颗粒对1例撕脱2 h且干燥保存的离体牙进行延迟再植、复位固定及后续牙髓治疗。随访1年,脱位牙恢复良好,达到牙周膜性愈合,没有出现牙根吸收,且牙槽骨高度得到恢复。

  20. Fibrin Adhesive: Clinical Application in Coronary Artery Bypass Graft Surgery

    Science.gov (United States)

    Fundaró, Pino; Velardi, Antonio R.; Santoli, Carmine

    1985-01-01

    Fibrin adhesive was used 72 times in a group of 67 patients undergoing elective coronary artery bypass graft surgery. The indications were prophylactic sealing of potential sources of bleeding, topical hemostasis (control of bleeding sites dangerous or difficult to suture), and fixation of the graft in the optimal position. The method of glue application under varying circumstances is described and the results are reported. This experience suggests that in some cases the glue expedites the operation and makes it safer. We conclude that the fibrin sealing represents a valid aid in coronary artery bypass graft surgery. PMID:15227018

  1. A Review of Bioceramics and Fibrin Sealant

    Directory of Open Access Journals (Sweden)

    Le Guéhennec L.

    2004-09-01

    Full Text Available This review focuses on bone substitute composites made by mixing ceramic biomaterials with fibrin sealants. Different biomaterials such as coral, bone-derived materials, bioactive glass ceramics, and synthetic calcium phosphate have been mixed with fibrin sealant, resulting in a combination of the biological properties of the two components. This type of association has not produced identical results in all studies. In the past for some, the addition of fibrin sealant to the biomaterial failed to produce any significant, positive effect on osteointegration, whereas others found a positive impact on bone colonization. Despite the negative biological effects reported previously, bioceramic-fibrin composites have been widely used in various types of bone surgery because they are easy to manipulate. In particular, the intra-operative preparation of these composites makes it possible to add bone growth factors or autologous osteoprogenitor cells prior to bone reconstruction. The bone growth factors and autologous osteoprogenitor cells associated with the bioceramic-fibrin composites should provide surgeons with tissue engineered grafts with enhanced osteointegrative properties. This review discusses both the advantages and disadvantages, as well as the future perspectives, of using bioceramic-fibrin composites in various clinical indications.

  2. Adipose tissue engineering with human adipose-derived stem cells and fibrin glue injectable scaffold%人脂肪来源干细胞复合纤维蛋白胶构建可注射型工程化脂肪组织的实验研究

    Institute of Scientific and Technical Information of China (English)

    张云松; 高建华; 鲁峰; 朱茗

    2008-01-01

    Objective To explore the possibility of building tissue-engineered adipose tissue and find a new approach for repairing soft tissue defects. Methods Using enzymatic digestion, adipose tissue derived stem cells (ASCs) were extracted from the lipid part of human liposuction aspirate, cultured, and underwent adipogenie induction or not. The adipegenie-induced and non-adipogenie-induced ASCs were labeled with 3, 3, 3', 3'-tetramcthylindo-carbecyanine perchlorate (DiI), a fluorescent marker, in vitro to be used as seed cells. Then, they were combined with injectable fibrin glue scaffold at 1×107/ml cell density. Six athymic BALB/C mice underwent subcutaneous injection of adipogonie-induced ASCs with fibrin glue scaffold at the density of 1×107 cells/ml into the left side of the low back (induced group), subcutaneous injection of non-adipegenic-inducad ASCs into the right side of the low back (non-induced group), and subcutaneous injection of injectable fibrin glue scaffold into the middle part of the neck (blank control group) ,with 0.2 ml per injection. Twelve weeks later the mice were killed and the implants were taken out. The wet weight was measured. HE and oil red O staining and light and fluorescence microscopy were used for morphological observation. Results Adipose tissue-like new-born tissues were found in the injection sites of the induced and un-induced groups. The average wet-weight of the induced group new-born tissue was (28±15) mg, significantly heavier than that of the un-induced group [(22±16) mg, P< 0.01]. HE staining and oil red O staining confirmed that the new-born tissue of the induced group was mature adipose tissue and DiI fluorescent staining approved its exogenousness. Most part of the new-born tissues of the un-induced group was fibroid tissue with only a few mature adipose tissues. Conclusion ASCs extracted from the lipid part after liposuction can be used as seed cells, mixed, after adipose-induction, with injectable scaffold of fibrin

  3. Cirugía oral en pacientes bajo terapia anticoagulante: modelo experimental para la evaluación de la eficacia de la cola de fibrina Oral surgery in patients treated with anticoagulating therapy: experimental model for evaluation of fibrin glue efficiency

    Directory of Open Access Journals (Sweden)

    G Taormina

    2004-06-01

    during oral surgery: it can avoid the risk of an excessive emorrhage and the necessity to alter the anticoagu- lating therapeutic regimen. The present study examines the fibrin glue emostatic ability as emostatic support in patients treated with anticoagulating therapy. It expresses the experiment results, based on standards of reference of surgical trauma and considers the anticoagulating therapy intensity. Materials and methods: 20 patients with anticoagulation therapy have been selected within the age of 44 and 79. Following the appropriate techniques extractions of teeth with one, two or three roots have been practiced. After the alveoloplasty a reabsorbable sponge of fibrine is placed and the suture is done. The surgical trauma has been classified by means of a quantitative scale which can express it without inaccuracy. The category of trauma for each patient is established by the total of the values obtained from each of the single procedures. It has been possible to evaluate the coefficient of relationship between the intensity of the anticoagulating effect (measured by the lNR and the number of verified hemorrhagic complications; and also between the grade of surgical trauma and the number of complications. Results and conclusions: The authors demonstrate how the therapeutical usage of fibrin glue can be considered a valid support for the optimization of the haemostatis in patients with induced alterations of the coagulation

  4. Preliminary investigation of a polyethylene glycol hydrogel "nerve glue"

    Directory of Open Access Journals (Sweden)

    McDaniel Candice

    2009-09-01

    Full Text Available Abstract Background Polyethylene glycol (PEG hydrogel is a biocompatible semi-adherent gel like substance that can potentially augment nerve repair much like a fibrin sealant. Potential advantages of this substance include fast preparation and set up time, as well as adhesion inhibiting properties. The purpose of this study was to perform an initial evaluation of PEG hydrogel in this application. Methods The sciatic nerves of 29 rats were transected and repaired using two 10-0 nylon sutures and either PEG hydrogel or fibrin glue. After 10 weeks, contraction forces of the reinnervated muscles were evaluated and histological assessment of scar tissue performed. Results Muscle strength testing revealed the average ratio of experimental to control sides for the fibrin glue group was 0.75 and for the PEG hydrogel group was 0.72 (no significant difference. Longitudinal sections through the nerve repair site showed no significant difference in nerve diameter but did demonstrate a significant reduction in scar thickness in the PEG hydrogel group (p Conclusion Though further study is necessary to fully evaluate, PEG hydrogel results in less scar tissue formation and equivalent muscle recovery as fibrin sealant when applied as a nerve glue in a rodent sciatic nerve repair model.

  5. Aulogous fibrin sealant (Vivostat ® in the neurosurgical practice: Part II: Vertebro-spinal procedures

    Directory of Open Access Journals (Sweden)

    Francesca Graziano

    2016-01-01

    Conclusions: In this preliminary study, the application of autologous fibrin sealant with Vivostat ® resulted in rapid hemostasis and/or acted as an effective dural sealant. Although this product appears to be safe and effective, further investigations are warranted.

  6. Platelet-rich fibrin versus albumin in surgical wound repair: a randomized trial with paired design

    DEFF Research Database (Denmark)

    Danielsen, Patricia L; Ågren, Sven Per Magnus; Jørgensen, Lars Nannestad

    2010-01-01

    To study the effects of autologous platelet-rich fibrin (PRF) versus human albumin on incisional wound breaking strength and subcutaneous collagen deposition in patients undergoing laparoscopic cholecystectomy in a randomized trial....

  7. Platelet-rich fibrin versus albumin in surgical wound repair: a randomized trial with paired design

    DEFF Research Database (Denmark)

    Danielsen, Patricia L; Ågren, Sven Per Magnus; Jørgensen, Lars Nannestad

    2010-01-01

    To study the effects of autologous platelet-rich fibrin (PRF) versus human albumin on incisional wound breaking strength and subcutaneous collagen deposition in patients undergoing laparoscopic cholecystectomy in a randomized trial.......To study the effects of autologous platelet-rich fibrin (PRF) versus human albumin on incisional wound breaking strength and subcutaneous collagen deposition in patients undergoing laparoscopic cholecystectomy in a randomized trial....

  8. Bioactivity and stability of endogenous fibrogenic factors in platelet-rich fibrin

    DEFF Research Database (Denmark)

    Lundquist, R.; Dziegiel, M.H.; Agren, M.S.

    2008-01-01

    Platelet-rich fibrin (PRF) is an autologous fibrin sealant (FS) enriched with a platelet concentrate (> 1,000,000 platelets/microL) produced by the automated Vivostat system and used to enhance wound healing. The effects of PRF were compared with supernatant from thrombin-activated platelet...

  9. Role of plasma-rich fibrin in oral surgery

    Science.gov (United States)

    Kumar, K. Retna; Genmorgan, K.; Abdul Rahman, S. M.; Rajan, M. Alaguvel; Kumar, T. Arul; Prasad, V. Srinivas

    2016-01-01

    Platelet-rich fibrin (PRF) is a fibrin meshwork, in which platelet cytokines, growth factors, and cells are entrapped and discharged after a period and can serve as a resorbable film. PRF is the next generation of platelet concentrates equipped to improve arrangement without biochemical blood handling; PRF is an evolution of the fibrin adhesive, which is widely used in the oral surgery. The guidelines of this innovation depend on concentrating platelets and growth factors in a plasma medium, and initiating them in a fibrin gel, keeping in mind the end goal to enhance the healing of wounds. Maxillary bone loss requires numerous regenerative techniques: as a supplement to the procedures of tissue regeneration, a platelet concentrate called PRF was tested for the 1st time in France by Dr. Choukroun. This article enriches the benefits and role of plasma-rich fibrin in oral surgery. Platelet-concentrate fibrin is an evolution of the fibrin glue, which is widely used in the oral surgery. PMID:27829743

  10. Role of plasma-rich fibrin in oral surgery

    Directory of Open Access Journals (Sweden)

    K Retna Kumar

    2016-01-01

    Full Text Available Platelet-rich fibrin (PRF is a fibrin meshwork, in which platelet cytokines, growth factors, and cells are entrapped and discharged after a period and can serve as a resorbable film. PRF is the next generation of platelet concentrates equipped to improve arrangement without biochemical blood handling; PRF is an evolution of the fibrin adhesive, which is widely used in the oral surgery. The guidelines of this innovation depend on concentrating platelets and growth factors in a plasma medium, and initiating them in a fibrin gel, keeping in mind the end goal to enhance the healing of wounds. Maxillary bone loss requires numerous regenerative techniques: as a supplement to the procedures of tissue regeneration, a platelet concentrate called PRF was tested for the 1st time in France by Dr. Choukroun. This article enriches the benefits and role of plasma-rich fibrin in oral surgery. Platelet-concentrate fibrin is an evolution of the fibrin glue, which is widely used in the oral surgery.

  11. Application of biomedical fibrin glue in pterygium excision combined with limbal stem cell transplantation%医用生物蛋白胶在翼状胬肉切除联合角膜缘干细胞移植术中的应用

    Institute of Scientific and Technical Information of China (English)

    肖津安; 任梅

    2011-01-01

    目的:探讨医用生物蛋白胶(biomedical fibrin glue,BFG)在翼状胬肉切除联合角膜缘干细胞移植术中应用的临床意义.方法:收集2009- 06/2010-12我院门诊翼状胬肉患者59例60眼,分为两组,使用生物蛋白胶组34眼和未使用蛋白胶对照组26眼,均行翼状胬肉切除联合角膜缘干细胞移植术,观察手术时间、术后结膜植片贴附情况,球结膜充血以及患者不适症状.结果:生物蛋白胶组手术时间(13.6±3.5min)明显短于对照组(22.3±5.2min),术后2wk内(拆线前),生物蛋白胶组术眼疼痛、异物感和流泪等局部刺激症状均显著轻于对照组,两组术后均未发生植片移位并发症.生物蛋白胶组术后干细胞移植片贴附紧密,对照组有5眼(19%)植片贴附差,出现结膜后退,巩膜外露.生物蛋白胶组植片愈合良好,局部无明显结膜瘢痕形成;对照组均出现不同程度的缝线刺激性结膜瘢痕.术后6mo随访期内两组均未见翼状胬肉复发.结论:医用生物蛋白胶在翼状胬肉切除联合角膜缘干细胞移植手术中的应用可以缩短结膜伤口愈合的时间,促进角膜缘干细胞结膜植片贴附,使用中未见致敏反应的产生.

  12. Electrophysiologic assessment of regeneration in rat sciatic nerve repair using suture, fibrin glue or a combination of both techniques Avaliação eletrofisiológica da eficácia de três tipos de reparo após a secção do nervo ciático do rato

    Directory of Open Access Journals (Sweden)

    Roberto Sergio Martins

    2005-09-01

    Full Text Available We evaluated the repair of seccioned rat sciatic nerve by the comparison of electrophysiologic parameters. The repair was effected with suture (group A, fibrin glue (group B or a combination of both techniques (group C. The amplitude, latency and conduction velocity of the motor and nerve action potentials were assessed before the nerve section and at reoperation after 24 weeks. There was no difference between the groups when the nerve action potential was evaluated. Rats of group B presented better results than those of group A (pForam comparados os parâmetros obtidos na avaliação eletrofisiológica do potencial de ação do nervo e do potencial de ação motor antes e após 24 semanas do reparo no nervo ciático do rato previamente seccionado no lado direito com a utilização de sutura (grupo A, adesivo de fibrina (grupo B ou uma combinação das duas técnicas (grupo C. Não houve diferença entre os grupos na avaliação do potencial de ação do nervo. Quando consideradas a latência e a velocidade de condução mensurados na reoperação e a razão entre a velocidade de condução medida na reoperação e o mesmo parâmetro antes da secção do nervo, durante a mensuração do potencial de ação motor, os animais do grupo B apresentaram melhores resultados em relação aos do grupo A (p<0,05. Os animais do grupo C apresentaram melhores resultados em comparação com os do grupo A quando considerada a razão entre a velocidade de condução medida 24 semanas do reparo e antes da secção do nervo durante a avaliação do potencial de ação motor. Conclui-se que os animais em que o reparo dos nervos foi realizado com o adesivo de fibrina apresentaram melhores resultados em comparação com a sutura quando considerados os parâmetros obtidos na mensuração do potencial de ação motor.

  13. Reduction of the closure time of postoperative enterocutaneous fistulas with fibrin sealant

    Institute of Scientific and Technical Information of China (English)

    Jorge; Avalos-González; Eliseo; Portilla-deBuen; Caridad; Aurea; Leal-Cortés; Abel; Orozco-Mosqueda; María; del; Carmen; Estrada-Aguilar; Gabriela; Abigail; Velázquez-Ramírez; Gabriela; Ambriz-González; Clotilde; Fuentes-Orozco; Aldo; Emmerson; Guzmán-Gurrola; Alejandro; González-Ojeda

    2010-01-01

    AIM: To assess whether the use of fibrin sealantshortens the closure time of postoperative enterocutaneous fistulas (ECFs). METHODS: The prospective case-control study included 70 patients with postoperative ECFs with an output of 2 cm and without any local complication. They were divided into study (n = 23) and control groups (n = 47). Esophageal, gastric and colocutaneous fistulas were monitored under endoscopic visualization, which also allowed fibrin glue application d...

  14. Enzymatically induced mineralization of platelet-rich fibrin.

    NARCIS (Netherlands)

    Douglas, T.E.L.; Gassling, V.; Declercq, H.A.; Purcz, N.; Pamula, E.; Haugen, H.J.; Chasan, S.; Mulder, E.L.W. de; Jansen, J.A.; Leeuwenburgh, S.C.G.

    2012-01-01

    Membranes of the autologous blood-derived biomaterial platelet-rich fibrin (PRF) were functionalized by incorporation of alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, and subsequently incubated in calcium glycerophosphate (CaGP) solution to induce PRFs mineralization with

  15. Albumen Glue, New Material for Conjunctival Graft Surgery, an Animal Experiment

    Science.gov (United States)

    Kartiwa, A.; Miraprahesti, R.; Sovani, I.; Enus, S.; Boediono, A.

    2017-02-01

    Attach conjunctival graft commonly used are suture technique and fibrin glue. This study was to investigate albumen glue as an alternative to suture technique in attaching conjunctival graft in rabbits. Aim of this study was to compare the conjunctival wound healing between albumen glue and suture technique in rabbit eye as a model. There was an experimental animal study included 32 eyes (16 rabbits) in PT. Bio Farma (Persero) and Histology Laboratory, Faculty of Medicine, Padjadjaran University from March 2014 to July 2104. The study consisted of albumen glue group and suture technique group. The examination included the comparison of conjunctival graft attachment and histologic examination by microscopically was done to obtain the wound gap, then analyze by Mann-Whitney test. The results indicated that the graft attachment was significantly better-using albumen glue (grade 4) compared to suture (grade 2-3) on day-1 after surgery (p=0,000). The wound gap was smaller using albumen glue (0-0,33 μm versus 5,33-14 μm ; p=0,0005) on 10 minutes after surgery and 0 μm versus 0,33-4 μm ; p=0,0005 on day-7 after surgery. In conclusion, the graft attachment using albumen glue was better and the wound gap was smaller using albumen glue than suture technique.

  16. Glue Guns: Aiming for Safety

    Science.gov (United States)

    Roy, Ken

    2010-01-01

    While glue guns are very useful, there are safety issues. Regardless of the temperature setting, glue guns can burn skin. The teacher should demonstrate and supervise the use of glue guns and have a plan should a student get burned. There should be an initial first aid protocol in place, followed by a visit to the school nurse. An accident report…

  17. 自体富血小板纤维蛋白复合脂肪干细胞对自体脂肪组织移植存活的影响%The effect of autologous platelet rich blood fibrin composite adipose-derived stem cells on the survival of autologous fat grafts

    Institute of Scientific and Technical Information of China (English)

    黄敏红; 黎洪棉; 梁至洁; 黄海; 张同韩; 黎宁; 池刚毅

    2015-01-01

    目的 观察自体富血小板纤维蛋白(PRF)复合脂肪干细胞(ASCs)对自体脂肪组织移植存活率影响.方法 取健康成年人下腹部脂肪组织颗粒进行体外分离培养扩增提取脂肪干细胞;同时抽取其静脉血20 ml,一次离心法提取白体PRF;制成4组混合物:实验组A: 100 g/L的PRF200μl+ASCs+脂肪组织(Fat) 500 mg;实验组B:生理盐水200μl+ASCs+ Fat 500 mg;实验组C:100 g/L PRF 200μl+Fat 500 mg混合而成;实验组D:生理盐水200 μl+ Fat 500 mg混合而成.在裸鼠背部中线两侧各分离2个皮下腔隙,将以上4组复合移植物各自随机注射入裸鼠的任一腔隙深筋膜下.移植3个月后将移植物取出,检测并对比各组脂肪移植物的微血管密度、脂肪成活率及脂肪细胞纤维化坏死率等.结果 统计学分析显示:各组脂肪细胞存活率分别为A组(78.5±6.2)%,B组(66.3±5.1)%,C组(63.8±5.5)%,D组(32.4±3.9)%.吸光率为A组(5.1±0.8)%,B组(3.5±0.4)%,C组(3.2±0.6)%,D组(1.3±0.3)%.脂肪细胞密度为A组(51.7 ±6.6)/mm2,B组(39.8 ±5.2)/mm2,C组(37.5 ±5.7)/mm2,D组(20.3 ±3.1)/mm2.脂肪组织微血管密度为A组(42.7 ±3.8)/mm2,B组(31.5±2.9)/mm2,C组(29.2 ±3.3)/mm2,D组(11.4 ±2.5)/mm2.A组的脂肪成活率(细胞存活率、脂滴量及细胞密度等)及微血管密度显著高于其他三组(P<0.05),而D组的脂肪成活率及微血管密度则显著低于其他三组(P<0.05).结论 早期应用PRF复合脂肪干细胞可促进移植脂肪组织局部的血管再生,增加脂肪组织的质量保持率,减少脂肪移植后的纤维坏死程度.%Objective To observe the improvement effect of platelet-rich fibrin (PRF) combine with adipose derived stem cells (ASCs) applied in autotransplantation on the survival rate of fat tissue transplantation.Methods ASCs were extracted from the lower abdominal adipose tissue particles of healthy adults and cultured in vitro, extracted the venous blood 20 ml from the same patient

  18. Concentrated red bone marrow/platelet-rich fibrin compound with an autologous periosteum debris scaffold for repair of mandibular defects%浓缩红骨髓/富血小板纤维蛋白复合载自体骨膜碎片修复下颌骨缺损★

    Institute of Scientific and Technical Information of China (English)

    郭灵雨; 刘凯; 刘树发

    2013-01-01

    BACKGROUND: The platelet-rich fibrin scaffold structure is conducive to the growth of red bone marrow mesenchymal stem cel s and various growth factors, promoting final osteogenesis. OBJECTIVE: To investigate the feasibility of the concentrated red bone marrow/platelet-rich fibrin compound with the autologous periosteum debris scaffold for repair of rabbit mandibular defects. METHODS: Bilateral mandibular bone defect models were prepared in New Zealand white rabbits using self-control method. The left side was the experimental side, and implanted with the concentrated red bone marrow/platelet-rich fibrin compound with the autologous periosteum debris scaffolds; the right side was the control side, and implanted with periosteum fragments and nano-hydroxyapatite composite scaffolds. Al experimental animals were sacrificed after 2, 4, 8, 12 weeks to prepare tissue samples for general observation, imaging analysis, hematoxylin-eosin staining and scanning electron microscope detection. RESULTS AND CONCLUSION: The imaging examination and histological staining showed that the experimental side was superior to the control side in the aspects of bone healing, osteogenesis speed and quality. Under the scanning electron microscope, the experimental side had better compatibility with surrounding tissues and no inflammation reaction. The data of the tooth CT and evidence of new bone formation showed the bone density and new bone area of the experimental side were significantly higher than that of the control side (P < 0.05). These data indicate that concentrated red bone marrow/platelet-rich fibrin compound with the autologous periosteum debris scaffolds has an obvious osteoinductive role, which is expected to be a new-type material for clinical repair of mandibular defects.%  背景:富血小板纤维蛋白支架结构有利于红骨髓中间充质干细胞及各种生长因子的生长,促进最终成骨。目的:探讨浓缩红骨髓/富血小板纤维蛋白复合

  19. [The initial trial of the clinical use of FK-1 glue in surgical interventions].

    Science.gov (United States)

    Abzhueva, O V; Rusanov, V M; Zhidkov, I L

    2000-01-01

    The article presents results of a comparative clinical trial of the preparation "fibrinous glue" (FK-1) and "Beriplast" in operations on the lungs for hermetization of the wound and the lung as well as in reconstructive plastic gynecological operations. Intraoperative hermetization in operations on the lungs was obtained in 67% of the cases when using "FK-1" and in 64% with "Beriplast". In all the cases the lung was spread during 12 hours after operation. The visual intraoperative assessment of using "FK-1" and control laparoscopy on the 4th-5th days after operation showed its good effect in gynecological procedures. Clinical trials of the new domestic fibrinous glue "FK-1" in surgical and gynecological clinics confirmed its high medical effectiveness.

  20. Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial.

    Science.gov (United States)

    Madurantakam, Parthasarathy; Yoganarasimha, Suyog; Hasan, Fadi K

    2015-09-29

    Autologous platelet concentrates represent promising innovative tools in the field of regenerative medicine and have been extensively used in oral surgery. Unlike platelet rich plasma (PRP) that is a gel or a suspension, Leukocyte-Platelet Rich Fibrin (L-PRF) is a solid 3D fibrin membrane generated chair-side from whole blood containing no anti-coagulant. The membrane has a dense three dimensional fibrin matrix with enriched platelets and abundant growth factors. L-PRF is a popular adjunct in surgeries because of its superior handling characteristics as well as its suturability to the wound bed. The goal of the study is to demonstrate generation as well as provide detailed characterization of relevant properties of L-PRF that underlie its clinical success.

  1. 纤维蛋白凝胶复合骨形态发生蛋白和庆大霉素缓释药物对感染性骨缺损的修复%Fibrin glue/bone morphogenetic protein complex plus slow-release gentamicin for repairing infected bone defects in rabbits

    Institute of Scientific and Technical Information of China (English)

    高秋明; 刘兴炎; 董晓萍; 葛宝丰; 白孟海; 陈克明

    2005-01-01

    背景:慢性骨髓炎临床处理较为棘手,手术常需分期进行,目前尚无好的方法予以一期修复.目的:探讨将纤维蛋白凝胶(FG)作为骨形态发生蛋白(BMP)及庆大霉素的共同载体,一期修复感染性骨缺损的可行性.设计:完全随机对照实验研究.单位:解放军兰州军区兰州总医院全军创伤骨科中心.材料:实验在兰州军区兰州总医院骨科研究所完成.对象为体质量1.9~2.4kg的48只成年健康青紫兰兔,雌雄不限,购自甘肃省兰州市生物制品研究所.干预:48只青紫兰兔,制作慢性骨髓炎模型,清创后造成胫骨近侧干骺端内侧1.5 cm长半环形骨缺损,采用3种方法进行处理:A组,植入FG,BMP和庆大霉素复合物;B组,植入FG/BMP复合物,C组,作为空白对照.主要观察指标:术后观察动物一般情况,做骨培养及细菌计数,X射线拍片及组织学检查.结果:A组感染控制及骨修复均良好,感染控制率、再生骨量明显优于B组.B,C两组在感染控制率上无显著差异.C组动物骨修复差.结论:FG,BMP及庆大霉素复合物具有促进成骨及抗感染的双重作用,可用于感染性骨缺损及污染严重的开放性损伤造成的骨缺损的修复.%BACKGROUND: Chronic osteomyelitis is difficult to manage clinically, and two or more operations were commonly needed. No satisfactory method for one-stage repair has been currently available.OBJECTIVE: To examine the possibility of using fibrin glue(FG) as the common carrier for both bone morphogenetic protein(BMP) and gentamicin for one-stage repair of infected bone defects.DESIGN: A completely randomized controlled experiment.SETTING: Center of Orthopaedic Surgery, Lanzhou General Hospital of Lanzhou Area Command of of Chinese PLA.MATERIALS: The experiment was conducted using 48 healthy adult Chinchilla rabbits of either sex on normal diet with body mass of 1.9 to 2.4 kg,provided by the Institute of Biological Products, Lanzhou, Gansu Province

  2. 21 CFR 178.3120 - Animal glue.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Animal glue. 178.3120 Section 178.3120 Food and... and Production Aids § 178.3120 Animal glue. Animal glue may be safely used as a component of articles..., transporting, or holding food, subject to the provisions of this section. (a) Animal glue consists of...

  3. BEWARE OF...SUPER GLUES!!

    CERN Multimedia

    2006-01-01

    What happened? A number of accidents have occurred with the use of 'Super Glues'. Some individuals have suffered injuries - severe irritation, or skin bonded together - through getting glue on their face and in their eyes. What are the hazards associated with glues? 'Super Glues' (i.e. cyanoacrylates): Are harmful if swallowed and are chemical irritants to the eyes, respiratory system and skin. Present the risk of polymerization (hardening) leading to skin damage. Be careful ! 'Super Glues' can bond to skin and eyes in seconds. Note: Other glues, resins and hardeners are also chemicals and as such can cause serious damage to the skin, eyes, respiratory or digestive tract. (For example: some components can be toxic, harmful, corrosive, sensitizing agents, etc.). How to prevent accidents in the future? Read the Material Safety Data Sheet (MSDS) for all of the glues you work with. Check the label on the container to find out which of the materials you work with are hazardous. Wear the right Per...

  4. Treatment of post-prostatectomy rectourethral fistula with fibrin sealant (Quixil™) injection: a novel application.

    Science.gov (United States)

    Verriello, V; Altomare, M; Masiello, G; Curatolo, C; Balacco, G; Altomare, D F

    2010-12-01

    Rectourethral fistulas in adults is a rare but potentially devastating postoperative condition requiring complex and demanding surgery. Fibrin glue treatment has been used with some success in anal and rectovaginal fistulas, and in the case we present here this indication has been extended to a postoperative rectourethral fistula following radical prostatectomy. For the first time, to our knowledge, a fibrin sealant (Quixil) was injected into the fistula tract, and a rectal mucosal flap was used to close the internal opening. The fistula healed in few weeks, and the patient is symptom free after 1 year of follow-up.

  5. [Healing of tubal anastomoses--microsurgery vs. fibrin gluing: morphologic aspects].

    Science.gov (United States)

    Gauwerky, J F; Klose, R P; Forssmann, W G

    1994-01-01

    The healing of anastomoses either by microsurgical suture technique or by fibrin sealant technique has been examined in an experimental morphological study. With view to morphological criteria the healing of tubal anastomoses after one month is completed. Afterwards only little areas of regeneration could be found in the region of the anastomosis. These statements are valid for both types of anastomoses. In single cases a more progressive regeneration of the mucosa could be demonstrated. Using fibrin glue a more pronounced fibrosis could not be seen.

  6. The contrastive research on the osteogenesis effects of recombinant human bone morphogenetic protein-2, platelet-rich fibrin and autologous bone compositeding with coralline hydroxyapatite respectively%rhBMP-2、PRF和自体骨分别复合珊瑚羟基磷灰石成骨效能的比较研究

    Institute of Scientific and Technical Information of China (English)

    宋亚平; 徐世同; 杨淑娟; 张彩美; 黄丞蔚; 刘虎

    2014-01-01

    目的:通过建立动物骨缺损模型,比较重组人骨形成蛋白-2(rhBMP-2)与珊瑚羟基磷灰石(CHA)复合物、富血小板纤维蛋白(PRF)与珊瑚羟基磷灰石复合物、自体骨与珊瑚羟基磷灰石复合物以及单纯珊瑚羟基磷灰石这四种骨移植材料在骨缺损中的成骨效能。方法:在比格犬双侧胫骨干骺端制备四个相同的骨缺损区,在缺损区分别植入rhBMP-2/CHA、 PRF/CHA、自体骨/CHA及CHA (对照);3个月后处死动物,行大体标本观察;拍牙科CT,观察各植骨区骨密度情况;制作石蜡切片、 HE染色,比较各植骨区骨组织学特点及新骨形成量。结果:大体标本见四组骨缺损间隙均完全关闭。 X线示自体骨/CHA组和PRF/CHA组骨密度较致密, rhBMP-2/CHA组致密性低于前两者, CHA组未见明显骨致密影。 HE切片见四组新生骨与宿主骨连接紧密,新生骨小梁不规则,粗细不一,排列无序;复合型骨移植材料的新生骨小梁比对照组更密集、粗大,连续性更好;四组植骨区成骨量比较:自体骨/CHA组>PRF/CHA组>rhBMP-2/CHA组>CHA组。结论:复合型骨移植材料成骨效应明显优于单纯珊瑚羟基磷灰石;三种复合型材料中自体骨/CHA成骨效应最好,其次为PRF/CHA, rhBMP-2/CHA最差。%Objective: By establishing bone defects animal model, the osteogenesis effects of four groups bone transplantation materials that they are compound of reco mbinant human bone morphogenetic protein-2 (rhBMP -2), coralline hydroxyapatite (CHA), compound of platelet -rich fibrin and coralline hydroxyapatite, compound of autologous bone and coralline hydroxyapatite and pure coralline hydroxyapatite were compared by repairing bone defects. Methods: Four same bone defects were prepared on each side of tibial metaphyseal of Beagles , rhBMP-2/CHA, PRF/CHA, autologous bone/CHA and pure CHA were respectively implanted into the bone defects. The Beagles

  7. [Perioperative salvage and use of autologous blood].

    Science.gov (United States)

    Larsen, B; Dich-Nielsen, J O

    1999-01-18

    Pre-operative blood donation gives ready availability of large volumes of patient compatible blood, up to four units and five when erythropoietin is used. It is recommended that autologous pre-donated blood is leucocyte depleted immediately after the donation. During normovolaemic haemodilution it is mandatory to monitor haemodynamics during the donation. Usually 1-2 units are removed pre-operatively and returned during or after the operation. Intra and postoperative salvage and recycling is performed either with washing and haemoconcentration of the blood or with salvage and immediate retransfusion. When salvaged blood is retransfused unwashed there are high levels of free haemoglobin, degradation products of fibrin/fibrinogen, interleukin-6 and activated complement. Clinically, this has not been shown to be of importance. Taking the patient's health status into account, we suggest that a level of B-haemoglobin should be determined pre-operatively to indicate use of transfusions both with autologous and allogeneic blood.

  8. Fibrin network architectures in pure platelet-rich plasma as characterized by fiber radius and correlated with clotting time.

    Science.gov (United States)

    Perez, Amanda G M; Rodrigues, Ana A; Luzo, Angela C M; Lana, José F S D; Belangero, William D; Santana, Maria H A

    2014-08-01

    Fibrin networks are obtained through activation of platelet-rich plasma (PRP) for use in tissue regeneration. The importance of fibrin networks relies on mediation of release of growth factors, proliferation of tissue cells and rheological properties of the fibrin gels. Activation of PRP usually involves the decomposition of fibrinogen by agonists, in a wide range of concentrations. Therefore fibrin networks with a large structural diversity are formed, making comparative evaluations difficult. In order to standardize the fibrin networks, we used the statistical techniques central composite rotatable design and response-surface analysis, to correlate the radius of the fibers with the ratios between the agonists (autologous serum/calcium chloride) and agonist/PRP. From an individual and interactive analysis of the variables, architectures characterized by thick, medium and thin fibers were delineated on the response-surface. Furthermore, the architectures were correlated with coagulation time. This approach is valuable for standardizing the PRP preparation for clinical applications.

  9. PLATELET RICH FIBRIN: A PROMISING INNOVATION IN REGENERATIVE THERAPY

    Directory of Open Access Journals (Sweden)

    Arun

    2015-04-01

    Full Text Available Platelets can play a crucial role in regenerative therapy as they are reservoirs of growth factors and cytokines which are the key factors for regeneration of the bone and maturation of the soft tissue. Platelet - rich fibr in (PRF was first described by Choukroun et al. in France. It has been referred to as a second - generation platelet concentrate, which has been shown to have several advantages over traditionally prepared PRP. Platelet - rich fibrin (PRF is autologous plate let concentrates prepared from patient’s own blood. It is a natural fibrin - based biomaterial prepared from an anticoagulant - free blood harvest without any artificial biochemical modification that allows obtaining fibrin membranes enriched with platelets a nd growth factors. Evidence from the literature suggests the potential role of PRF in regeneration and tissue engineering. The slow polymerisation during centrifugation and fibrin - based structure makes PRF a better healing biomaterial than PRP and other fi brin adhesives. The purpose of this review article is to describe the novel second - generation platelet concentrate PRF, which is an improvement over the traditionally prepared PRP for use in regenerative dentistry.

  10. Platelet rich fibrin: a new paradigm in periodontal regeneration.

    Science.gov (United States)

    Kumar, R Vinaya; Shubhashini, N

    2013-09-01

    Among the great challenges facing clinical research is the development of bioactive surgical additives regulating inflammation and increasing healing. Although the use of fibrin adhesives and platelet-rich plasma (PRP) is well documented, they have their own limitations. Hence, reconstructive dental surgeons are looking for an "edge" that jump starts the healing process to maximize predictability as well as the volume of regenerated bone. Overcoming the restrictions related to the reimplantation of blood-derived products, a new family of platelet concentrate, which is neither a fibrin glue nor a classical platelet concentrate, was developed in France. This second generation platelet concentrate called platelet-rich fibrin (PRF), has been widely used to accelerate soft and hard tissue healing. Its advantages over the better known PRP include ease of preparation/application, minimal expense, and lack of biochemical modification (no bovine thrombin or anticoagulant is required). This article serves as an introduction to the PRF "concept" and its potential clinical applications with emphasis on periodontal regeneration.

  11. Treatment of osteochondral defects in the rabbit's knee joint by implantation of allogeneic mesenchymal stem cells in fibrin clots.

    Science.gov (United States)

    Berninger, Markus T; Wexel, Gabriele; Rummeny, Ernst J; Imhoff, Andreas B; Anton, Martina; Henning, Tobias D; Vogt, Stephan

    2013-05-21

    bone (11). The sandwich-technique combines bone grafting with current approaches in Tissue Engineering (5,6). This combination seems to be able to overcome the limitations seen in osteochondral grafts alone. After autologous bone grafting to the subchondral defect area, a membrane seeded with autologous chondrocytes is sutured above and facilitates to match the topology of the graft with the injured site. Of course, the previous bone reconstruction needs additional surgical time and often even an additional surgery. Moreover, to date, long-term data is missing (12). Tissue Engineering without additional bone grafting aims to restore the complex structure and properties of native articular cartilage by chondrogenic and osteogenic potential of the transplanted cells. However, again, it is usually only the cartilage tissue that is more or less regenerated. Additional osteochondral damage needs a specific further treatment. In order to achieve a regeneration of the multilayered structure of osteochondral defects, three-dimensional tissue engineered products seeded with autologous/allogeneic cells might provide a good regeneration capacity (11). Beside autologous chondrocytes, mesenchymal stem cells (MSC) seem to be an attractive alternative for the development of a full-thickness cartilage tissue. In numerous preclinical in vitro and in vivo studies, mesenchymal stem cells have displayed excellent tissue regeneration potential (13,14). The important advantage of mesenchymal stem cells especially for the treatment of osteochondral defects is that they have the capacity to differentiate in osteocytes as well as chondrocytes. Therefore, they potentially allow a multilayered regeneration of the defect. In recent years, several scaffolds with osteochondral regenerative potential have therefore been developed and evaluated with promising preliminary results (1,15-18). Furthermore, fibrin glue as a cell carrier became one of the preferred techniques in experimental cartilage

  12. Successful closure of a bronchopleural fistula by intrapleural administration of fibrin sealant: A case report with review of Literature

    Directory of Open Access Journals (Sweden)

    Pranabh Shrestha

    2014-01-01

    Full Text Available Context: There are no established guidelines for the proper treatment of patients with bronchopleural fistulas (BPFs. Apart from attempts to close the fistula, emphasis of treatment and management is placed on preventive measures, early administration of antibiotics, drainage of the empyema and aggressive nutritional and rehabilitative support. Case Report: A 53-year-old male presented with nausea, vomiting, and dry cough with eventual respiratory failure. He was found to have an empyema of the left hemithorax which was managed with thoracostomy drainage and antibiotics. However, he had persistent air leak through the chest tube due to a BPF. Bronchoscopy failed to localize the involved segment. Application of fibrin glue through the chest tube succeeded in completely sealing the leak. Conclusion: To our knowledge, this is the first case report in which fibrin glue was successfully used intrapleurally to close a BPF related to an empyema.

  13. Fibrin clot formation and lysis: basic mechanisms

    DEFF Research Database (Denmark)

    Sidelmann, JJ; Gram, J; Jespersen, J;

    2000-01-01

    consequently is an important substrate in the physiology of hemostasis. This review describes the components and processes involved in fibrin formation and fibrin degradation. Particular emphasis is put on the reactions involved in the conversion of fibrinogen to fibrin, the polymerization of fibrin molecules...... induced by coagulation factor XIII (FXIII), and the degradation of fibrinogen and fibrin mediated by plasmin and elastase. Furthermore, factors influencing fibrin structure and fibrin breakdown are addressed; in particular polymorphisms in the genes coding for fibrinogen and FXIII, but also the physical...

  14. Fabrication and physical and biological properties of fibrin gel derived from human plasma.

    Science.gov (United States)

    Zhao, Haiguang; Ma, Lie; Zhou, Jie; Mao, Zhengwei; Gao, Changyou; Shen, Jiacong

    2008-03-01

    The fast development of tissue engineering and regenerative medicine drives the old biomaterials, for example, fibrin glue, to find new applications in these areas. Aiming at developing a commercially available hydrogel for cell entrapment and delivery, in this study we optimized the fabrication and gelation conditions of fibrin gel. Fibrinogen was isolated from human plasma by a freeze-thaw circle. Gelation of the fibrinogen was accomplished by mixing with thrombin. Absorbance of the fibrinogen/thrombin mixture at 550 nm as a function of reaction time was monitored by UV-VIS spectroscopy. It was found that the clotting time is significantly influenced by the thrombin concentration and the temperature, while less influenced by the fibrinogen concentration. After freeze-drying, the fibrin gel was characterized by scanning electron microscopy (SEM), revealing fibrous microstructure. Thermal gravimetric analysis found that the degradation temperature of the crosslinked fibrin gel starts from 288 degrees C, which is about 30 degrees C higher than that of the fibrinogen. The hydrogel has an initial water-uptake ratio of approximately 50, decreased to 30-40 after incubation in water for 11 h depending on the thrombin concentration. The fibrin gels lost their weights in PBS very rapidly, while slowly in DMEM/fetal bovine serum and DMEM. In vitro cell culture found that human fibroblasts could normally proliferate in the fibrin gel with spreading morphology. In conclusion, the fibrin gel containing higher concentration of fibrinogen (20 mg ml(-1)) and thrombin (5 U ml(-1)) has suitable gelation time and handling properties, and thus is applicable as a delivery vehicle for cells such as fibroblasts.

  15. Hyaluronan and Fibrin Biomaterial as Scaffolds for Neuronal Differentiation of Adult Stem Cells Derived from Adipose Tissue and Skin

    Directory of Open Access Journals (Sweden)

    Chiara Gardin

    2011-10-01

    Full Text Available Recently, we have described a simple protocol to obtain an enriched culture of adult stem cells organized in neurospheres from two post-natal tissues: skin and adipose tissue. Due to their possible application in neuronal tissue regeneration, here we tested two kinds of scaffold well known in tissue engineering application: hyaluronan based membranes and fibrin-glue meshes. Neurospheres from skin and adipose tissue were seeded onto two scaffold types: hyaluronan based membrane and fibrin-glue meshes. Neurospheres were then induced to acquire a glial and neuronal-like phenotype. Gene expression, morphological feature and chromosomal imbalance (kariotype were analyzed and compared. Adipose and skin derived neurospheres are able to grow well and to differentiate into glial/neuron cells without any chromosomal imbalance in both scaffolds. Adult cells are able to express typical cell surface markers such as S100; GFAP; nestin; βIII tubulin; CNPase. In summary, we have demonstrated that neurospheres isolated from skin and adipose tissues are able to differentiate in glial/neuron-like cells, without any chromosomal imbalance in two scaffold types, useful for tissue engineering application: hyaluronan based membrane and fibrin-glue meshes.

  16. In-vitro-Studie zum Einfluss von Fibrin in Knorpelkonstrukten auf der Basis von PGA-Vliesstoffen

    DEFF Research Database (Denmark)

    Schmal, H; Mehlhorn, A T; Kurze, C

    2008-01-01

    and cultured using a serum-free technique. The cells were seeded on PGA-91 scaffolds with and without an additional fibrin component; the constructs were cultured for 2 weeks in vitro. Besides cell viability, DNA content, pH, aggrecan production, mRNA expression of aggrecan, and collagen types I and II were...... remained within a physiologic range. The Alcian blue staining of the constructs showed the homogeneous cell distribution and a cell-associated proteoglycan production. Aggrecan concentration in the supernatants of fibrin-containing constructs was significantly lower compared to fibrin-free grafts (-24......BACKGROUND: The matrix component in autologous chondrocyte implantation plays an important role. In this study the influence of an additional fibrin component in cartilage constructs based on polyglycolide polymers (PGA) was investigated. METHODS: Human chondrocytes of femoral heads were isolated...

  17. One-step apexification using platelet rich fibrin matrix and mineral trioxide aggregate apical barrier

    Directory of Open Access Journals (Sweden)

    Anisha Kumar

    2014-01-01

    In one-step apexification using MTA, the technical problem encountered is controlling the overfill or underfill of MTA. The use of a matrix material helps to overcome this shortcoming. Platelet rich fibrin (PRF is an immune platelet concentrate, which can be used as a matrix, it also promotes wound healing and repair. This case report presents a case of one step apexification using MTA as an apical barrier and autologous PRF as an internal matrix.

  18. Close up of the glue dispenser. The white lines on the green frames are theapplied glue.

    CERN Multimedia

    Bernd Surrow

    1999-01-01

    The gantry motion control along with the pneumatic glue dispenser system allows precise control of the amount of glue applied to theframe. The glue is a special room temperature cure silicone basedglue chosen to minimize surface stresses on the silicon sensors dueto differential temperature contraction.

  19. Autologous adipose tissue-derived stem cells treatment demonstrated favorable and sustainable therapeutic effect for Crohn's fistula.

    Science.gov (United States)

    Lee, Woo Yong; Park, Kyu Joo; Cho, Yong Beom; Yoon, Sang Nam; Song, Kee Ho; Kim, Do Sun; Jung, Sang Hun; Kim, Mihyung; Yoo, Hee-Won; Kim, Inok; Ha, Hunjoo; Yu, Chang Sik

    2013-11-01

    Fistula is a representative devastating complication in Crohn's patients due to refractory to conventional therapy and high recurrence. In our phase I clinical trial, adipose tissue-derived stem cells (ASCs) demonstrated their safety and therapeutic potential for healing fistulae associated with Crohn's disease. This study was carried out to evaluate the efficacy and safety of ASCs in patients with Crohn's fistulae. In this phase II study, forty-three patients were treated with ASCs. The amount of ASCs was proportioned to fistula size and fistula tract was filled with ASCs in combination with fibrin glue after intralesional injection of ASCs. Patients without complete closure of fistula at 8 weeks received a second injection of ASCs containing 1.5 times more cells than the first injection. Fistula healing at week 8 after final dose injection and its sustainability for 1-year were evaluated. Healing was defined as a complete closure of external opening without any sign of drainage and inflammation. A modified per-protocol analysis showed that complete fistula healing was observed in 27/33 patients (82%) by 8 weeks after ASC injection. Of 27 patients with fistula healing, 26 patients completed additional observation study for 1-year and 23 patients (88%) sustained complete closure. There were no adverse events related to ASC administration. ASC treatment for patients with Crohn's fistulae was well tolerated, with a favorable therapeutic outcome. Furthermore, complete closure was well sustained. These results strongly suggest that autologous ASC could be a novel treatment option for the Crohn's fistula with high-risk of recurrence.

  20. Simultaneous sinus lift and implantation using platelet-rich fibrin as sole grafting material.

    Science.gov (United States)

    Jeong, Seung-Mi; Lee, Chun-Ui; Son, Jeong-Seog; Oh, Ji-Hyeon; Fang, Yiqin; Choi, Byung-Ho

    2014-09-01

    Recently, several authors have shown that simultaneous sinus lift and implantation using autologous platelet-rich fibrin as the sole filling material is a reliable procedure promoting bone augmentation in the maxillary sinus. The aim of this study was to examine the effect of simultaneous sinus lift and implantation using platelet-rich fibrin as the sole grafting material on bone formation in a canine sinus model. An implant was placed after sinus membrane elevation in the maxillary sinus of six adult female mongrel dogs. The resulting space between the membrane and sinus floor was filled with autologous platelet-rich fibrin retrieved from each dog. The implants were left in place for six months. Bone tissue was seen at the lower part of the implants introduced into the sinus cavity. The height of the newly formed bone around the implants ranged from 0 mm to 4.9 mm (mean; 2.6 ± 2.0 mm) on the buccal side and from 0 mm to 4.2 mm (mean; 1.3 ± 1.8 mm) on the palatal side. The findings from this study suggest that simultaneous sinus lift and implantation using platelet-rich fibrin as sole grafting material is not a predictable and reproducible procedure, especially with respect to the bone formation around the implants in the sinus cavity.

  1. 复合PRP的可注射型组织工程骨修复兔桡骨缺损的实验研究%Injectable tissue-engineered bone made of autologous platelet-rich plasma, fibrin glue and BMSCs for repairing segmental radial defect in rabbits

    Institute of Scientific and Technical Information of China (English)

    黄爱文; 金丹; 裴国献; 曾宪利; 胡稷杰; 林海宁

    2007-01-01

    目的 以自体富血小板血浆(platelet-rich plasma, PRP)、骨髓基质干细胞(bone marrow stromal cells, BMSCs)和纤维蛋白胶构建可注射型组织工程骨并探讨其修复骨缺损的效果.方法 36只新西兰兔分为A、B、C 3组,每组12只.A、B组动物术前4 h抽取耳背中央动脉血提取PRP. A组于术前1~2个月抽取双髂骨处骨髓并培养出BMSCs,在体外与纤维蛋白胶(FG)及自体PRP构建成可注型组织工程骨,植入自体桡骨1.5 cm节段性骨缺损,为实验组.B、C两组分别植入PRP+FG、FG于同样骨缺损处为对照组.另取4只桡骨同样部位和大小骨缺损旷置作为空白组.术后观察其一般情况并于4、8、12周取材做组织学切片,术后12周取尺桡骨做生物力学测试.分别从组织学观察、生物力学方面评估比较骨缺损的修复情况.结果 组织学观察见A、B组各时间点新骨形成均优于C组.生物力学比较:12周时A组桡骨生物力学强度与正常桡骨比较无明显差异(P>0.05),但其明显优于B组(P<0.05).结论 PRP对骨缺损愈合有明显促进作用,复合PRP的可注射型骨修复材料及构建的含种子细胞的组织工程骨均可修复节段性骨缺损,但种子细胞的添加可明显促进新骨成熟度和增强其生物力学性能.

  2. Platelet rich fibrin in jaw defects

    Science.gov (United States)

    Nica, Diana; Ianes, Emilia; Pricop, Marius

    2016-03-01

    Platelet rich fibrin (PRF) is a tissue product of autologous origin abundant in growth factors, widely used in regenerative procedures. Aim of the study: Evaluation of the regenerative effect of PRF added in the bony defects (after tooth removal or after cystectomy) Material and methods: The comparative nonrandomized study included 22 patients divided into 2 groups. The first group (the test group) included 10 patients where the bony defects were treated without any harvesting material. The second group included 12 patients where the bony defects were filled with PRF. The bony defect design was not critical, with one to two walls missing. After the surgeries, a close clinically monitoring was carried out. The selected cases were investigated using both cone beam computer tomography (CBCT) and radiographic techniques after 10 weeks postoperatively. Results: Faster bone regeneration was observed in the bony defects filled with PRF comparing with the not grafted bony defects. Conclusions: PRF added in the bony defects accelerates the bone regeneration. This simplifies the surgical procedures and decreases the economic costs.

  3. Role of Fibrin Sealants in Liver Surgery

    NARCIS (Netherlands)

    de Boer, Marieke T.; Boonstra, Elizabeth A.; Lisman, Ton; Porte, Robert J.

    2012-01-01

    Background: Fibrin sealants are widely used in liver surgery. The aim of this article is to review the literature on evidence of hemostatic and biliostatic capacities of different fibrin sealants in liver surgery. Methods: In PubMed, a literature search was done with the search terms 'fibrin sealant

  4. Spiders Tune Glue Viscosity to Maximize Adhesion.

    Science.gov (United States)

    Amarpuri, Gaurav; Zhang, Ci; Diaz, Candido; Opell, Brent D; Blackledge, Todd A; Dhinojwala, Ali

    2015-11-24

    Adhesion in humid conditions is a fundamental challenge to both natural and synthetic adhesives. Yet, glue from most spider species becomes stickier as humidity increases. We find the adhesion of spider glue, from five diverse spider species, maximizes at very different humidities that matches their foraging habitats. By using high-speed imaging and spreading power law, we find that the glue viscosity varies over 5 orders of magnitude with humidity for each species, yet the viscosity at maximal adhesion for each species is nearly identical, 10(5)-10(6) cP. Many natural systems take advantage of viscosity to improve functional response, but spider glue's humidity responsiveness is a novel adaptation that makes the glue stickiest in each species' preferred habitat. This tuning is achieved by a combination of proteins and hygroscopic organic salts that determines water uptake in the glue. We therefore anticipate that manipulation of polymer-salts interaction to control viscosity can provide a simple mechanism to design humidity responsive smart adhesives.

  5. Growth factor and proteinase profile of Vivostat® platelet-rich fibrin linked to tissue repair

    DEFF Research Database (Denmark)

    Ågren, Sven Per Magnus; Rasmussen, Karina; Pakkenberg, Bente

    2014-01-01

    BACKGROUND AND OBJECTIVES: Autologous platelet-rich fibrin (PRF(®)) is prepared by the automatic Vivostat(®) system. Conflicting results with Vivostat PRF in acute wound healing prompted us to examine its cellular and biomolecular composition. Specifically, platelets, selected growth factors...... and matrix metalloproteinase (MMP)-9 were quantified using novel analytical methods. MATERIALS AND METHODS: Ten healthy non-thrombocytopenic volunteers donated blood for generation of intermediate fibrin-I and final PRF. Anticoagulated whole blood and serum procured in parallel served as baseline controls....... Leucocyte, erythrocyte and platelet counts in whole blood and fibrin-I were determined by automated haematology analyser. Platelet concentration in PRF was quantified manually by stereologic analysis of Giemsa-stained tissue sections, and the total content of five growth factors and MMP-9 by enzyme...

  6. A new heterologous fibrin sealant as a scaffold to cartilage repair-Experimental study and preliminary results.

    Science.gov (United States)

    de Barros, Caio Nunes; Miluzzi Yamada, Ana Lúcia; Junior, Rui Seabra F; Barraviera, Benedito; Hussni, Carlos Alberto; de Souza, Jaqueline Brandão; Watanabe, Marcos Jun; Rodrigues, Celso Antônio; Garcia Alves, Ana Liz

    2016-07-01

    Autologous fibrin gel is commonly used as a scaffold for filling defects in articular cartilage. This biomaterial can also be used as a sealant to control small hemorrhages and is especially helpful in situations where tissue reparation capacity is limited. In particular, fibrin can act as a scaffold for various cell types because it can accommodate cell migration, differentiation, and proliferation. Despite knowledge of the advantages of this biomaterial and mastery of the techniques required for its application, the durability of several types of sealant at the site of injury remains questionable. Due to the importance of such data for evaluating the quality and efficiency of fibrin gel formulations on its use as a scaffold, this study sought to analyze the heterologous fibrin sealant developed from the venom of Crotalus durissus terrificus using studies in ovine experimental models. The fibrin gel developed from the venom of this snake was shown to act as a safe, stable, and durable scaffold for up to seven days, without causing adverse side effects. Fibrin gel produced from the venom of the Crotalus durissus terrificus snake possesses many clinical and surgical uses. It presents the potential to be used as a biomaterial to help repair skin lesions or control bleeding, and it may also be used as a scaffold when applied together with various cell types. The intralesional use of the fibrin gel from the venom of this snake may improve surgical and clinical treatments in addition to being inexpensive and adequately consistent, durable, and stable. The new heterologous fibrin sealant is a scaffold candidate to cartilage repair in this study.

  7. Platelet rich fibrin and xenograft in treatment of intrabony defect

    Directory of Open Access Journals (Sweden)

    Saurav Panda

    2014-01-01

    Full Text Available For complete periodontal regeneration, delivery of growth factors in the local environment holds a great deal in adjunct to bone grafts. Platelet rich fibrin (PRF is considered as second generation platelet concentrate, consisting of viable platelets, releasing various growth factors such as platelet-derived growth factor, vascular endothelial growth factor, transforming growth factor, insulin-like growth factor, epidermal growth factor and basic fibroblast growth factor. Hence, this case report aims to investigate the clinical and radiological (bone fill effectiveness of autologous PRF along with the use of xenogenic bone mineral in the treatment of intra bony defects. Intrabony defect was treated with autologous PRF along with the use of xenogenic bone mineral. A decrease in probing pocket depth, gain in clinical attachment level and significant bone fill was observed at end of 6 months. The result obtained with the use of PRF may be attributed to the sustained and simultaneous release of various growth factors over a period of 7 days. In this case report, the positive clinical impact of additional application of PRF with xenogenic graft material in the treatment of periodontal intrabony defect was seen.

  8. Bioactivity and stability of endogenous fibrogenic factors in platelet-rich fibrin

    DEFF Research Database (Denmark)

    Lundquist, Rasmus; Dziegiel, Morten H; Agren, Magnus S

    2008-01-01

    Platelet-rich fibrin (PRF) is an autologous fibrin sealant (FS) enriched with a platelet concentrate (> 1,000,000 platelets/microL) produced by the automated Vivostat system and used to enhance wound healing. The effects of PRF were compared with supernatant from thrombin-activated platelet...... concentrate, recombinant human platelet-derived growth factor (rhPDGF) isoforms, and a homologous FS in cultured normal human dermal fibroblasts. Also, the release of selected endogenous growth factors from PRF and their stability against proteolytic degradation were studied. The proliferative effect of PRF...... exceeded that of FS and rhPDGF-BB, although it was lower than thrombin-activated platelet concentrate possibly due to sustained growth factor release from platelets in PRF. Anti-PDGF antibody blocked the mitogenic effect of rhPDGF-BB but not that of PRF in growth-arrested fibroblasts. PRF promoted...

  9. Randomized clinical trial of fibrin glue versus tacked fixation in laparoscopic groin hernia repair

    DEFF Research Database (Denmark)

    Tolver, Mette A; Rosenberg, Jacob; Juul, Poul;

    2013-01-01

    of moderate/severe nausea and/or vomiting, foreign-body sensation, and recurrence after 6 months. Outcome measures were assessed by visual analogue scale (VAS, 0-100 mm), verbal rating scale (no, light, moderate or severe) and numerical rating scales (NRS, 1-10). RESULTS: One hundred patients were available...

  10. Successful management of chylous ascites with total parenteral nutrition, somatostatin, and fibrin glue

    Institute of Scientific and Technical Information of China (English)

    HUANG Qi; GE Bu-jun; LIU Li-ming; TU Zhi-yuan; ZHANG Guo-fen; FAN Yue-zu

    2007-01-01

    @@ Chylous ascites, an uncommon disease usually caused by obstruction or rupture of the peritoneal or retroperitoneal lymphatic glands, is defined as the accumulation of chyle in the peritoneal cavity.

  11. Sealing of Gastrointestinal Anastomoses with a Fibrin Glue-Coated Collagen Patch: A Safety Study

    DEFF Research Database (Denmark)

    Nordentoft, Tyge; Rømer, John; Sørensen, Michael

    2007-01-01

    for anastomotic leakage, signs of present or former peritonitis, abscess, adhesions to the anastomoses, and signs of intestinal obstruction. In addition, the anastomotic diameter was measured with barium and radiography. Finally, bursting pressure was measured in each segment. After the pigs were sacrificed...

  12. DayAND#8211;Care Surgery for Pilonidal Sinus Using Sinotomy Technique and Fibrin Glue Injection

    Directory of Open Access Journals (Sweden)

    Selim Sozen

    2012-06-01

    Conclusion: An ideal surgical procedure for a pilonidal sinus should be one aiming at reducing hospital stay, minimizing tissue assault, promoting early work resumption, and preventing recurrence. The method described in this paper was found to be simple, safe, and feasible as day-care surgery. The technique of sinotomy with a good wound and surrounding skin care is an ideal approach with a high chance of cure. The patients returned to their routine within a short duration of time. [Arch Clin Exp Surg 2012; 1(3.000: 138-141

  13. The GlueX DIRC project

    Science.gov (United States)

    Stevens, J.; Barbosa, F.; Bessuille, J.; Chudakov, E.; Dzhygadlo, R.; Fanelli, C.; Frye, J.; Hardin, J.; Kelsey, J.; Patsyuk, M.; Schwartz, C.; Schwiening, J.; Shepherd, M.; Whitlatch, T.; Williams, M.

    2016-07-01

    The GlueX experiment was designed to search for and study the pattern of gluonic excitations in the meson spectrum produced through photoproduction reactions at a new tagged photon beam facility in Hall D at Jefferson Laboratory. The particle identification capabilities of the GlueX experiment will be enhanced by constructing a DIRC (Detection of Internally Reflected Cherenkov light) detector, utilizing components of the decommissioned BaBar DIRC. The DIRC will allow systematic studies of kaon final states that are essential for inferring the quark flavor content of both hybrid and conventional mesons. The design for the GlueX DIRC is presented, including the new expansion volumes that are currently under development.

  14. Hackable User Interfaces In Astronomy with Glue

    Science.gov (United States)

    Beaumont, C.; Goodman, A.; Greenfield, P.

    2015-09-01

    Astronomers typically choose between Graphical User Interfaces and custom-written computer code when exploring and analyzing data. Few tools are designed to encourage both of these workflows, despite their complementary strengths. We believe that such hybrid hackable user interfaces could enable more agile data exploration, combining the fluidity that comes from a GUI with the precision and reproducibility that comes from writing code. In this article we articulate the different strengths and weaknesses of both workflows and discuss how to enable both in a single tool. We focus on Glue (http://glue-viz.org) as a case study and examine how the goal of creating a hackable user interface has influenced the design of Glue.

  15. The GlueX DIRC Project

    CERN Document Server

    Stevens, Justin; Bessuille, Jason; Chudakov, Eugene; Dzhygadlo, Roman; Fanelli, Cristiano; Frye, John; Hardin, John; Kelsey, Jim; Patsyuk, Maria; Schwartz, Carsten; Schwiening, Jochen; Shepherd, Matthew; Whitlatch, Tim; Williams, Michael

    2016-01-01

    The GlueX experiment was designed to search for and study the pattern of gluonic excitations in the meson spectrum produced through photoproduction reactions at a new tagged photon beam facility in Hall D at Jefferson Laboratory. The particle identification capabilities of the GlueX experiment will be enhanced by constructing a DIRC (Detection of Internally Reflected Cherenkov light) detector, utilizing components of the decommissioned BaBar DIRC. The DIRC will allow systematic studies of kaon final states that are essential for inferring the quark flavor content of both hybrid and conventional mesons. The design for the GlueX DIRC is presented, including the new expansion volumes that are currently under development.

  16. Principle of Melt-glue Cloth

    Institute of Scientific and Technical Information of China (English)

    陈人豪; 曹建达; 李济群; 张利梅; 何洋

    2003-01-01

    This paper advances a new concept of textile-meltglue cloth, and introduces the readers to the basic principle of melt-glue cloth. On the basis of melt spinning, the spinneret can be replaced by a spinning device that consists of an outer spinneret (a loop)and an inner spinneret ( a round plate), and between them there is an interval circle on which the centers of the holes are evenly distributed. When the machine is running, the outer spinneret (or the inner one) is fixed, the inner spinneret (or the outer one)is spinning, and a columnar net will be obtained.Then it will be excided with the help of a cutter in transporting it. Finally the once-forming melt-glue cloth will be produced. Compared with the traditional woven fabric, melt-glue cloth has a lot of special features and a bright future of application.

  17. Laparoscopic intraperitoneal mesh fixation with fibrin sealant (Tisseel®) vs. titanium tacks: a randomised controlled experimental study in pigs

    DEFF Research Database (Denmark)

    Eriksen, J.R.; Bech, Jakob Ilsted; Linnemann, D.

    2008-01-01

    chronic) pain after LVHR. Therefore, non-invasive and patient-friendly mesh fixation methods must be considered. The present study was designed to investigate the technical applicability, safety and effect of Tisseel (R) for intraperitoneal mesh fixation. Methods Nine 40-kg Danish Landrace female pigs had...... two pieces of MotifMESH (R) and two pieces of Proceed (R) mesh fixed in the intraperitoneal position by a laparoscopic technique. The two pieces of the same mesh were fixed with fibrin glue (Tisseel) and titanium tacks, respectively. All pigs were euthanised on the 30th postoperative day and the mesh...... feasible in a pig model. There is still no evidence that fibrin-sealing alone is appropriate for intraperitoneal mesh fixation in hernia repair, but the technique might become an alternative or supplement to mechanical mesh fixation. Until then, further experimental research in animal hernia models...

  18. Laparoscopic intraperitoneal mesh fixation with fibrin sealant (Tisseel((R))) vs. titanium tacks: a randomised controlled experimental study in pigs

    DEFF Research Database (Denmark)

    Eriksen, J.R.; Bech, J.I.; Linnemann, D.

    2008-01-01

    chronic) pain after LVHR. Therefore, non-invasive and patient-friendly mesh fixation methods must be considered. The present study was designed to investigate the technical applicability, safety and effect of Tisseel((R)) for intraperitoneal mesh fixation. METHODS: Nine 40-kg Danish Landrace female pigs...... had two pieces of MotifMESH((R)) and two pieces of Proceed((R)) mesh fixed in the intraperitoneal position by a laparoscopic technique. The two pieces of the same mesh were fixed with fibrin glue (Tisseel) and titanium tacks, respectively. All pigs were euthanised on the 30th postoperative day...... with Tisseel is safe and technically feasible in a pig model. There is still no evidence that fibrin-sealing alone is appropriate for intraperitoneal mesh fixation in hernia repair, but the technique might become an alternative or supplement to mechanical mesh fixation. Until then, further experimental...

  19. Platelet-rich fibrin: Evolution of a second-generation platelet concentrate

    Directory of Open Access Journals (Sweden)

    Sunitha Raja V

    2008-01-01

    Full Text Available Platelet-rich plasma (PRP is a platelet concentrate that has been used widely to accelerate soft-tissue and hard-tissue healing. The preparation of PRP has been described by several authors. Platelet-rich fibrin (PRF was first described by Choukroun et al. in France. It has been referred to as a second-generation platelet concentrate, which has been shown to have several advantages over traditionally prepared PRP. Its chief advantages include ease of preparation and lack of biochemical handling of blood, which makes this preparation strictly autologous. This article describes the evolution of this novel platelet concentrate, referred to as PRF.

  20. Photoproduction of JPsi at GlueX

    Science.gov (United States)

    Seth, Kamal; GlueX Collaboration Collaboration

    2016-09-01

    We propose to measure photoproduction of J / ψ using the photon beam at GlueX produced by 12 GeV electrons. Existing measurements at SLAC lead to the expectation that J / ψ can be produced at GlueX with photon energy ranging from 8.4-11.8 GeV. The measurement will enable us to distinguish between J / ψ production due to two or three gluons interacting with the proton target. Data taken recently with 12 GeV electrons is being analyzed to confirm the expected production of J / ψ decaying into e+e- .

  1. Autologous Costochondral Microtia Reconstruction.

    Science.gov (United States)

    Patel, Sapna A; Bhrany, Amit D; Murakami, Craig S; Sie, Kathleen C Y

    2016-04-01

    Reconstruction with autologous costochondral cartilage is one of the mainstays of surgical management of congenital microtia. We review the literature, present our current technique for microtia reconstruction with autologous costochondral graft, and discuss the evolution of our technique over the past 20 years. We aim to minimize donor site morbidity and create the most durable and natural appearing ear possible using a stacked framework to augment the antihelical fold and antitragal-tragal complex. Assessment of outcomes is challenging due to the paucity of available objective measures with which to evaluate aesthetic outcomes. Various instruments are used to assess outcomes, but none is universally accepted as the standard. The challenges we continue to face are humbling, but ongoing work on tissue engineering, application of 3D models, and use of validated questionnaires can help us get closer to achieving a maximal aesthetic outcome.

  2. Cohesin: A Multi-purpose Chromatin Glue

    Institute of Scientific and Technical Information of China (English)

    Laura A. D(i)az-Mart(i)nez; Hongtao Yu

    2009-01-01

    Long thought to be the glue responsible for holding sister chromatids together, cohesin has been found to be stickier than previously thought. Recent discoveries point to cohesin having a role in transcription regulation by mediating long-distance intra-chromosomal interactions.

  3. Comparison of commercial fibrin sealants in facelift surgery: a prospective study

    Directory of Open Access Journals (Sweden)

    Botti G

    2013-11-01

    Full Text Available Giovanni Botti,1 Michele Pascali,2 Chiara Botti,1 Florian Bodog,3 Pietro Gentile,2 Valerio Cervelli2 1Villa Bella Clinic, Salò, 2Department of Plastic and Reconstructive Surgery, University of Rome Tor Vergata, Italy; 3University of Oradea, Oradea, Romania Background: The aim of this study was to compare the effects of two types of fibrin glue in patients undergoing facelift surgery. Methods: A prospective, controlled "right-left side" study was carried out in 20 patients. The two fibrin sealants used were Quixil® and Tissucol®. The two sealants were used at the same time, ie, one on one side of the face and the other on the contralateral side. Comparisons were made with regard to rates of hematoma and seroma, degree of induration, edema, ecchymosis, pain levels, and patient satisfaction. Results: The results were almost equivalent. The only exception was a significant (40 mL hematoma in a patient treated with Quixil. Bleeding was most likely due to a sudden rise in blood pressure during the immediate postoperative period. However, it must be emphasized that, while Tissucol actually seals the undermined area, thus virtually eliminating the dead space, Quixil acts differently, in that its effectiveness in preventing hematoma is linked mainly to its hemostatic effect. Conclusion: The two fibrin sealants used were nearly identical with regard to patient safety and quality of the result. Nevertheless, it is noted that, while Tissucol has both hemostatic and "gluing" effects, Quixil is mainly effective in securing hemostasis. Keywords: facelift surgery, rhytidectomy, fibrin sealants, hematoma

  4. Influence of platelet-derived growth factor-AB on tissue development in autologous platelet-rich plasma gels.

    Science.gov (United States)

    Wirz, Simone; Dietrich, Maren; Flanagan, Thomas C; Bokermann, Gudrun; Wagner, Wolfgang; Schmitz-Rode, Thomas; Jockenhoevel, Stefan

    2011-07-01

    Fibrin-based scaffolds are widely used in tissue engineering. We postulated that the use of platelet-rich plasma (PRP) in contrast to platelet-poor plasma and pure fibrinogen as the basic material leads to an increased release of autologous platelet-derived growth factor (PDGF)-AB, which may have a consequent positive effect on tissue development. Therefore, we evaluated the release of PDGF-AB during the production process and the course of PDGF release during cultivation of plasma gels with and w/o platelets. The influence of PDGF-AB on the proliferation rate of human umbilical cord artery smooth muscle cells (HUASMCs) was studied using XTT assay. The synthesis of extracellular matrix by HUASMCs in plasma- and fibrin gels was measured using hydroxyproline assay. The use of PRP led to an increase in autologous PDGF-AB release. Further, the platelet-containing plasma gels showed a prolonged release of growth factor during cultivation. Both PRP and platelet-poor plasma gels had a positive effect on the production of collagen. However, PDGF-AB as a supplement in medium and in pure fibrin gel had neither an effect on cell proliferation nor on the collagen synthesis rate. This observation may be due to an absence of PDGF receptors in HUASMCs as determined by flow cytometry. In conclusion, although the prolonged autologous production of PDGF-AB in PRP gels is possible, the enhanced tissue development by HUASMCs within such gels is not PDGF related.

  5. Fibrin ring granulomas in hepatitis A.

    Science.gov (United States)

    Ruel, M; Sevestre, H; Henry-Biabaud, E; Courouce, A M; Capron, J P; Erlinger, S

    1992-12-01

    Fibrin ring granuloma is characterized by a fibrinous ring surrounding a central fat vacuole. It has been found in the liver and bone marrow of patients with Q fever, and occasionally with visceral leishmaniasis, cytomegalovirus, Epstein-Barr virus, Staphylococcus epidermidis infections, Hodgkin's lymphoma, and hypersensitivity to allopurinol. We describe a case of serologically confirmed viral hepatitis A with this lesion in the liver biopsy. A false positive anti-hepatitis A virus IgM result has been excluded. This is, to our knowledge, the second reported case of type A hepatitis with hepatic fibrin ring granulomas. It confirms that hepatitis A should be included in the differential diagnosis of this lesion.

  6. 医用生物蛋白胶对注射型骨形态发生蛋白成骨活性的影响%Influence of fibrin sealant on osteoinductive ability of inject-type bone morphogenetic protein

    Institute of Scientific and Technical Information of China (English)

    王登虎; 刘建; 李丹; 胡蕴玉; 袁志

    2002-01-01

    Objective To observe influence of fibrin sealant(FS) on osteoinductive ability of inject type BMP.Method The inject type BMP power was dissolved in the main glue part or thrombin part of FS, then mixed with the main glue part or thrombin part of FS into gel, observe coagulating time, then implant composite into the thigh muscle pouch of mice to evaluate their capacity to induce new bone formation, and compared to the single BMP implant group.Result There was no difference in the coagulating time between two mixing method, the osteoinductive ability of implants BMP dissolved in the main glue part or thrombin part of FS group was higher than that of simply BMP implant group.Conclusion FS was perfect carrier to inject type BMP.

  7. The adjuvant use of stromal vascular fraction and platelet-rich fibrin for autologous adipose tissue transplantation%可注射自体脂肪颗粒复合PRF和S VF促进移植脂肪组织再生

    Institute of Scientific and Technical Information of China (English)

    谭新颖; 刘斌; 刘彦普; 李龙; 徐海燕; 安然

    2014-01-01

    目的:探讨PRF和SVF对自体脂肪颗粒移植的作用,并分析这种可注射复合生物材料的移植效果.方法:健康新西兰家兔24只,分为四组:单纯脂肪组织移植组(2 mL AG+0.2 mL NS)、脂肪颗粒复合富血小板纤维蛋白(2 mL AG+0.2 mL PRF)组、脂肪颗粒复合基质血管成分(2 mL AG+0.2 mL SVF)组和脂肪颗粒复合PRF和SVF[2 mL AG+0.2 mL (SVF+PRF)]组.术后1、3、6月取材,进行大体观察、组织病理学检测、游标卡尺和B超对移植脂肪组织的体积测量.采用SPSS16.0软件,对四组移植脂肪组织的存活率及吸收率进行方差分析并采用LSD法对不同时间点组间进行比较.结果:24周后各组植入脂肪组织的吸收率分别为 AG +NS [(49.4±9.5)%],AG+SVF [(27.2±4.4)%],AG+PRF [(36.4±8.5)%]和AG+SVF+PRF [(17.4±6.2)%],并有显著差异(P<0.01).结论:实验结果表明自体脂肪组织复合PRF和SVF能够促进移植脂肪组织的再生,为临床脂肪组织移植提供一种新的策略.%AIM:This study was to explore the role of PRF and SVF on autologous adipose granule transplant,and analyze the effect of the transplantation of the injectable compound biological material.METHODS:24 healthy New Zealand rabbits were di-vided into four groups:2 ml adipose granules and 0.2 ml normal saline solution (AG+NS group),2 ml adipose granules and 0.2 ml SVF (AG+SVF group),2 ml adipose granules and 0.2 ml PRF (AG+PRF group)or 2 ml adipose granules combined with 0.1 ml SVF and 0.1 ml PRF (AG+SVF+PRF group).1,3,6 months after the operation,each group was taken gross observa-tion,histological examination,and was measured the volume of transplanted adipose tissue by Vernier caliper and B ultrasound. All data collected were expressed as means ±standard deviation (SD).Statistical analysis was performed by One-Way ANOVA and LSD method using SPSS 16

  8. Structural hierarchy governs fibrin gel mechanics.

    Science.gov (United States)

    Piechocka, Izabela K; Bacabac, Rommel G; Potters, Max; Mackintosh, Fred C; Koenderink, Gijsje H

    2010-05-19

    Fibrin gels are responsible for the mechanical strength of blood clots, which are among the most resilient protein materials in nature. Here we investigate the physical origin of this mechanical behavior by performing rheology measurements on reconstituted fibrin gels. We find that increasing levels of shear strain induce a succession of distinct elastic responses that reflect stretching processes on different length scales. We present a theoretical model that explains these observations in terms of the unique hierarchical architecture of the fibers. The fibers are bundles of semiflexible protofibrils that are loosely connected by flexible linker chains. This architecture makes the fibers 100-fold more flexible to bending than anticipated based on their large diameter. Moreover, in contrast with other biopolymers, fibrin fibers intrinsically stiffen when stretched. The resulting hierarchy of elastic regimes explains the incredible resilience of fibrin clots against large deformations.

  9. Laryngospasm after autologous blood transfusion.

    Science.gov (United States)

    Hong, Jung; Grecu, Loreta

    2006-07-01

    Although perioperative autologous blood transfusions are associated with few side effects, transfusion reactions can occur and can be life-threatening. We report the occurrence of postoperative laryngospasm in a patient who underwent spinal anesthesia for hip surgery. The laryngospasm could not be attributed to any cause other than the autologous blood transfusion and recurred when the transfusion was restarted. Laryngospasm was successfully treated both times with positive pressure ventilation. Autologous transfusions can trigger febrile nonhemolytic transfusion reactions, which may result in airway compromise.

  10. 纤维蛋白胶的临床应用进展%Progress of clinical application on tibrin glue

    Institute of Scientific and Technical Information of China (English)

    殷香保; 王捷

    2003-01-01

    @@ 纤维蛋白胶(Fibrin Glue,简称FG)是一种生物蛋白制剂,最早应用于伤口止血.近20年来,随着手术要求的不断提高,FG也逐渐应用于促进伤口愈合、封闭组织缺损、防止组织粘连,甚至作为某些药物的缓释载体试用于临床.其使用方法不断改进,应用范围不断拓展.本文就FG的临床应用作一综述.

  11. Thrombin interaction with fibrin polymerization sites.

    Science.gov (United States)

    Hsieh, K

    1997-05-15

    Thrombin is central to hemostasis, and postclotting fibrinolysis and wound healing. During clotting, thrombin transforms plasma fibrinogen into polymerizing fibrin, which selectively adsorbs the enzyme into the clot. This protects thrombin from heparin-antithrombin inactivation, thus preserving the enzyme for postclotting events. To determine how the fibrin N-terminal polymerization sites of A alpha 17-23 (GPRVVER) and B beta 15-25 (GHRPLDKKREE) and their analogs may interact with thrombin, amidolysis vs. plasma- and fibrinogen-clotting assays were used to differentiate blockade of catalytic site vs. other thrombin domains. Amidolysis studies suggest GPRVVER inhibition of thrombin catalytic site through hydrophobic interaction, and GPRVVER inhibited clotting. Neither GPRP nor VVER nor the B beta 15-25 homologs inhibited amidolysis. Contrary to heparin, acyl-DKKREE promoted plasma-clotting, but inhibited fibrinogen-clotting. In addition, acyl-DKKREE reversed the anticoagulant effect of heparin (0.1 U/ml) in plasma. The results suggest fibrin B beta 15-25 interaction with thrombin, possibly by blocking the heparin-binding site. Together with the reported fibrin A alpha 27-50 binding to thrombin, polymerizing fibrin appears to initially bind to thrombin catalytic site and exosite-1 through A alpha 17-50, and to another thrombin site through B beta 15-25. As these fibrin sites are also involved in polymerization, competition of the polymerization process with thrombin-binding could subsequently dislodge thrombin from fibrin alpha-chain. This may re-expose the catalytic site and exosite-1, thus explaining the thrombogenicity of clot-bound thrombin. The implications of these findings in polymerization mechanism and anticoagulant design are discussed.

  12. Obtention of injectable platelets rich-fibrin (i-PRF) and its polymerization with bone graft: technical note.

    Science.gov (United States)

    Mourão, Carlos Fernando de Almeida Barros; Valiense, Helder; Melo, Elias Rodrigues; Mourão, Natália Belmock Mascarenhas Freitas; Maia, Mônica Diuana-Calasans

    2015-01-01

    The use of autologous platelet concentrates, represent a promising and innovator tools in the medicine and dentistry today. The goal is to accelerate hard and soft tissue healing. Among them, the platelet-rich plasma (PRP) is the main alternative for use in liquid form (injectable). These injectable form of platelet concentrates are often used in regenerative procedures and demonstrate good results. The aim of this study is to present an alternative to these platelet concentrates using the platelet-rich fibrin in liquid form (injectable) and its use with particulated bone graft materials in the polymerized form.

  13. Obtention of injectable platelets rich-fibrin (i-PRF and its polymerization with bone graft: technical note

    Directory of Open Access Journals (Sweden)

    Carlos Fernando de Almeida Barros Mourão

    2015-12-01

    Full Text Available The use of autologous platelet concentrates, represent a promising and innovator tools in the medicine and dentistry today. The goal is to accelerate hard and soft tissue healing. Among them, the platelet-rich plasma (PRP is the main alternative for use in liquid form (injectable. These injectable form ofplatelet concentrates are often used in regenerative procedures and demonstrate good results. The aim of this study is to present an alternative to these platelet concentrates using the platelet-rich fibrin in liquid form (injectable and its use with particulated bone graft materials in the polymerized form.

  14. 21 CFR 864.7300 - Fibrin monomer paracoagulation test.

    Science.gov (United States)

    2010-04-01

    ... to detect fibrin monomer in the diagnosis of disseminated intravascular coagulation (nonlocalized clotting within a blood vessel) or in the differential diagnosis between disseminated intravascular coagulation and primary fibrinolysis (dissolution of the fibrin in a blood clot). (b) Classification. Class...

  15. Fibrin sealant for mesh fixation in laparoscopic umbilical hernia repair

    DEFF Research Database (Denmark)

    Eriksen, J R; Bisgaard, T; Assaadzadeh, S;

    2013-01-01

    Fibrin sealant for mesh fixation has significant positive effects on early outcome after laparoscopic ventral hernia repair (LVHR) compared with titanium tacks. Whether fibrin sealant fixation also results in better long-term outcome is unknown.......Fibrin sealant for mesh fixation has significant positive effects on early outcome after laparoscopic ventral hernia repair (LVHR) compared with titanium tacks. Whether fibrin sealant fixation also results in better long-term outcome is unknown....

  16. GlueX: Meson Spectroscopy in Photoproduction

    Energy Technology Data Exchange (ETDEWEB)

    Salgado, Carlos [Norfolk State University, JLAB; Smith, Elton S. [JLAB

    2014-03-01

    The goal of the GlueX experiment \\cite{gluex} is to provide crucial data to help understand the soft gluonic fields responsible for binding quarks in hadrons. Hybrid mesons, and in particular exotic hybrid mesons, provide the ideal laboratory for testing QCD in the confinement regime since these mesons explicitly manifest the gluonic degrees of freedom. Photoproduction is expected to be effective in producing exotic hybrids but there is little data on the photoproduction of light mesons. GlueX will use the new 12-GeV electron beam at Jefferson Lab to produce a 9-GeV beam of linearly polarized photons using the technique of coherent bremsstrahlung. A solenoid-based hermetic detector is under construction, which will be used to collect data on meson production and decays. These data will also be used to study the spectrum of conventional mesons, including the poorly understood excited vector mesons. This talk will give an update on the experiment as well as describe theoretical developments \\cite{Dudek:2011bn} to help understand how these data can provide insights into the fundamental theory of strong interactions.

  17. Bioinspired Nanoparticulate Medical Glues for Minimally Invasive Tissue Repair.

    Science.gov (United States)

    Lee, Yuhan; Xu, Chenjie; Sebastin, Monisha; Lee, Albert; Holwell, Nathan; Xu, Calvin; Miranda Nieves, David; Mu, Luye; Langer, Robert S; Lin, Charles; Karp, Jeffrey M

    2015-11-18

    Delivery of tissue glues through small-bore needles or trocars is critical for sealing holes, affixing medical devices, or attaching tissues together during minimally invasive surgeries. Inspired by the granule-packaged glue delivery system of sandcastle worms, a nanoparticulate formulation of a viscous hydrophobic light-activated adhesive based on poly(glycerol sebacate)-acrylate is developed. Negatively charged alginate is used to stabilize the nanoparticulate surface to significantly reduce its viscosity and to maximize injectability through small-bore needles. The nanoparticulate glues can be concentrated to ≈30 w/v% dispersions in water that remain localized following injection. With the trigger of a positively charged polymer (e.g., protamine), the nanoparticulate glues can quickly assemble into a viscous glue that exhibits rheological, mechanical, and adhesive properties resembling the native poly(glycerol sebacate)-acrylate based glues. This platform should be useful to enable the delivery of viscous glues to augment or replace sutures and staples during minimally invasive procedures.

  18. Stent-and-glue sutureless vascular anastomosis.

    Science.gov (United States)

    Khorgami, Zhamak; Shoar, Saeed; Aminian, Ali; Nasiri, Shirzad; Mahmoodzadeh, Habibollah

    2011-07-01

    Vascular anastomosis is commonly done by hand-sewn methods which not only are slow in pace, but also need experiences in surgeons' hands. As the old techniques are replaced by the new ones all the time, it is sensed that a new sutureless approach should be welcomed in the field of vascular anastomosis. Although lots of efforts have been done, such previous recommended techniques are associated with adverse consequences and here is where the need for new methods is still sensed. In this manuscript, we bring all the benefits from other methods together and conclude a novel one for end-to-end vascular anastomosis which uses biological glue as connecting material and also an absorbable stent to keep vessel patency while using balloon catheter and tacking suture.

  19. Molecular, cellular and pharmaceutical aspects of autologous grafts for peri-implant hard and soft tissue defects.

    Science.gov (United States)

    Lu, Jiayu; Hao, Yongming; Zhao, Wei; Lv, Chengqi; Zou, Derong

    2016-12-01

    The lack of supporting hard and soft tissues always prevents the rehabilitation with dental implants. Among various hard and soft tissue augmentation procedures, autologous grafts have been considered to be the gold standard. Autologous mesenchymal stem cells (MSCs) from bone marrow, dental tissue and adipose tissue have been described as promising alternatives for bone regeneration in the field of dental implantation. Mucosal cells, gingival fibroblasts and dental progenitor cells (DPS) can enhance peri-implant soft tissue augmentation and regenerate periodontal tissues around dental implants. Obtained from patients, platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are enriched in autologous platelets, which contain a great deal of growth factors and cytokines that are conducive to the regeneration of both hand and soft tissues around dental implants. Pharmaceutical treatments for osteoporosis and diabetes should be locally applied with implant procedures to restrict the resorption of autologous bone grafts and reduction of bone volume. Although autografts hold great potentials for dental implants, new approaches should also be explored with minimally invasion donor sites methods such as tissue engineering combined with autologous three factors and bio-3D printing involving self-assembling cell aggregates.

  20. UROKINASE MUTANT WITH BETTER FIBRIN SPECIFICITY

    Institute of Scientific and Technical Information of China (English)

    MaZhong; YuRuirong; HuaZichun; ZhuDexu

    1997-01-01

    Urokinsae (UK),one of the principal activators of plasminogen, has been widely used in the clinical therapy of thrombosis. However ,it lacks fibrin specificity ,and can lead to extensive systemic activation of plasminogen when injected into veins in large doses. On the other hand,

  1. Platelet-rich fibrin: the benefits.

    Science.gov (United States)

    Kumar, Yuvika Raj; Mohanty, Sujata; Verma, Mahesh; Kaur, Raunaq Reet; Bhatia, Priyanka; Kumar, Varun Raj; Chaudhary, Zainab

    2016-01-01

    Current published data presents confusing results about the effects of platelet-rich fibrin on bone, and there is a need for studies that throw light on its effect. Our main objective therefore was to evaluate (by fractal analysis) osseous regeneration in extraction sockets with and without platelet-rich fibrin in a study with a substantial sample and a reliable technique to calibrate its effects on bone cells. We also assessed the soft tissue response. Thirty-four patients had their bilaterally impacted third molars (68 surgical sites) extracted in this split-mouth study, following which platelet-rich fibrin was placed in one of the sockets. Patients were followed up clinically and radiographically, and a pain score and fractal analysis were used to evaluate healing of soft tissue and bone, respectively. We conclude that platelet-rich fibrin improves healing of both soft and hard tissues. Although osseous healing did not differ significantly between the groups, healing of soft tissue as judged by the pain score was significantly better in the experimental group.

  2. Osteogenic potential of human umbilical cord-derived mesenchymal stromal cells cultured with umbilical cord blood-derived fibrin: a preliminary study.

    Science.gov (United States)

    Baba, Kyoko; Yamazaki, Yasuharu; Ishiguro, Masashi; Kumazawa, Kenichi; Aoyagi, Kazuya; Ikemoto, Shigehiro; Takeda, Akira; Uchinuma, Eiju

    2013-12-01

    This study examined the potential for osteogenesis via regenerative medicine using autologous tissues (umbilical cord (UC) and umbilical cord blood (UCB)) in nude mice. The study was designed to provide the three elements required for regenerative medicine (cell, scaffold, and growth factor) and autoserum for culture by means of autologous tissues. Mesenchymal stromal cells were obtained from UC (UC-MSCs). Fibrin, platelet-rich-plasma, and autoserum were obtained from UCB as scaffold, growth factor and serum for culture respectively. UC-MSCs were obtained from Wharton jelly and cultured with UCB-derived fibrin (UCB-fibrin) for 3-4 weeks to induce their differentiation into osteoblasts. They were implanted subcutaneously into the dorsum of male nude mice for 6 weeks prior to undergoing assessment. The assessments performed were haematoxylin and eosin, and alizarin red staining, immunohistochemical staining of human mitochondria, scanning electron microscopy, scanning electron microscopy with energy dispersive X-ray spectrometry and real-time reverse transcriptase-polymerase chain reaction to assess the expressions of osteoblast markers. Consequently, the differentiation of UC-MSCs into osteoblasts and the production of hydroxyapatite were verified. This study suggested the possible formation of bone tissue using biomedical materials obtained from UC and UCB.

  3. Clinical evaluation and comparison of the efficacy of coronally advanced flap alone and in combination with platelet rich fibrin membrane in the treatment of Miller Class I and II gingival recessions

    OpenAIRE

    Santosh Gupta; Ruchi Banthia; Pallavi Singh; Priyank Banthia; Sapna Raje; Neha Aggarwal

    2015-01-01

    Background: The aim of this study was to compare the clinical efficacy of coronally advanced flap (CAF) alone and in combination with autologous platelet rich fibrin membrane (PRF) in Miller′s class I and II gingival recessions. Materials and Method: Thirty isolated Miller class I or II sites in 26 subjects were randomly divided into test (15 sites- CAF+PRF) and control (15 sites- CAF alone). Parameters probing pocket depth (PPD), Recession depth (RD), Clinical attachment loss (CAL), Keratini...

  4. Platelet-rich fibrin is a Bioscaffold and reservoir of growth factors for tissue regeneration.

    Science.gov (United States)

    Kang, Young-Ho; Jeon, Soung Hoo; Park, Joo-Young; Chung, Jong-Hoon; Choung, Yun-Hoon; Choung, Han-Wool; Kim, Eun-Suk; Choung, Pill-Hoon

    2011-02-01

    The platelet-rich fibrin (PRF) is known as a rich source of autologous cytokines and growth factors and universally used for tissue regeneration in current clinical medicine. However, the microstructure of PRF has not been fully investigated nor have been studied the key molecules that differ PRF from platelet-rich plasma. We fabricated PRF under Choukroun's protocol and produced its extract (PRFe) by freezing at -80°C. The conventional histological, immunohistological staining, and scanning electron microscopy images showed the microstructure of PRF, appearing as two zones, the zone of platelets and the zone of fibrin, which resembled a mesh containing blood cells. The PRFe increased proliferation, migration, and promoted differentiation of the human alveolar bone marrow stem cells (hABMSCs) at 0.5% concentration in vitro. From the results of proteome array, matrix metalloproteinase 9 (MMP9) and Serpin E1 were detected especially in PRFe but not in concentrated platelet-rich plasma. Simultaneous elevation of MMP9, CD44, and transforming growth factor β-1 receptor was shown at 0.5% PRFe treatment to the hABMSC in immunoblot. Mineralization assay showed that MMP9 directly regulated mineralization differentiation of hABMSC. Transplantation of the fresh PRF into the mouse calvarias enhanced regeneration of the critical-sized defect. Our results strongly support the new characteristics of PRF as a bioscaffold and reservoir of growth factors for tissue regeneration.

  5. In vivo evaluation of titanium-prepared platelet-rich fibrin (T-PRF): a new platelet concentrate.

    Science.gov (United States)

    Tunalı, Mustafa; Özdemir, Hakan; Küçükodacı, Zafer; Akman, Serhan; Fıratlı, Erhan

    2013-07-01

    We have developed a new, titanium-prepared, platelet-rich fibrin (T-PRF) together with the protocol for forming it, which is based on the hypothesis that titanium tubes may be more effective at activating platelets than the glass tubes used by Chouckroun in his platelet-rich fibrin (PRF) method. The aim of this study was to find a suitable animal model in which to evaluate the method and to investigate the efficacy of T-PRF for wound healing. Blood samples from 6 rabbits were used to confirm the protocol for formation of T-PRF. We evaluated T-PRF or T-PRF-like clots morphologically using scanning electron microscopy (EM). Blood samples from 5 rabbits were used to develop an experiment in which to evaluate the effects of T-PRF on wound healing. The mucoperiosteal flaps were filled with autologous T-PRF membranes from the vestibule in the anterior mandibular regions. Samples collected from the surgical sites were stained with haematoxylin and eosin. We found a mature fibrin network in T-PRF clots that had been centrifuged for 15 min at 3500 rpm and, 15 days after placement of the membrane, we found newly-forming connective tissue and islets of bony tissue in the T-PRF membrane. These results show that T-PRF could induce the formation of new bone with new connective tissue in a rabbit model of wound healing within 30 days of treatment.

  6. High-throughput proteomic characterization of plasma rich in growth factors (PRGF-Endoret)-derived fibrin clot interactome.

    Science.gov (United States)

    Anitua, Eduardo; Prado, Roberto; Azkargorta, Mikel; Rodriguez-Suárez, Eva; Iloro, Ibon; Casado-Vela, Juan; Elortza, Felix; Orive, Gorka

    2015-11-01

    Plasma rich in growth factors (PRGF®-Endoret®) is an autologous technology that contains a set of proteins specifically addressed to wound healing and tissue regeneration. The scaffold formed by using this technology is a clot mainly composed of fibrin protein, forming a three-dimensional (3D) macroscopic network. This biomaterial is easily obtained by biotechnological means from blood and can be used in a range of situations to help wound healing and tissue regeneration. Although the main constituent of this clot is the fibrin scaffold, little is known about other proteins interacting in this clot that may act as adjuvants in the healing process. The aim of this study was to characterize the proteins enclosed by PRGF-Endoret scaffold, using a double-proteomic approach that combines 1D-SDS-PAGE approach followed by LC-MS/MS, and 2-DE followed by MALDI-TOF/TOF. The results presented here provide a description of the catalogue of key proteins in close contact with the fibrin scaffold. The obtained lists of proteins were grouped into families and networks according to gene ontology. Taken together, an enrichment of both proteins and protein families specifically involved in tissue regeneration and wound healing has been found.

  7. Preparation of hybrid scaffold from fibrin and biodegradable polymer fiber.

    Science.gov (United States)

    Hokugo, Akishige; Takamoto, Tomoaki; Tabata, Yasuhiko

    2006-01-01

    A biodegradable hybrid scaffold was prepared from fibrin and poly(glycolic acid) (PGA) fiber. Mixed fibrinogen and thrombin solution homogeneously dispersed in the presence of various amounts (0, 1.5, 3.0, and 6.0mg) of PGA fiber was freeze-dried to obtain fibrin sponges with or without PGA fiber incorporation. By scanning electron microscopy observation, the fibrin sponges had an interconnected pore structure, irrespective of the amount of PGA fiber incorporated. PGA fiber incorporation enabled the fibrin sponges to significantly enhance their compression strength. In vitro cell culture studies revealed that the number of L929 fibroblasts initially attached was significantly larger for any fibrin sponge with PGA fiber incorporation than for the fibrin sponge without PGA fiber. The shrinkage of sponges after cell seeding was suppressed by fiber incorporation. It is possible that the shrinkage suppression of sponges maintains their intraspace, resulting in the superior cell attachment of a sponge with PGA fiber incorporation. After subcutaneous implantation into the backs of mice, the residual volume of a fibrin sponge with PGA fiber incorporation was significant compared with that of a fibrin sponge without PGA fiber. Larger number of cells infiltrated deep inside the fibrin sponges with PGA fiber incorporation implanted subcutaneously. It is concluded that the fibrin sponge reinforced by fiber incorporation is a promising three-dimensional scaffold of cells for tissue engineering.

  8. Platelet rich fibrin matrix effects on skeletal muscle lesions: an experimental study.

    Science.gov (United States)

    Gigante, A; Del Torto, M; Manzotti, S; Cianforlini, M; Busilacchi, A; Davidson, P A; Greco, F; Mattioli-Belmonte, M

    2012-01-01

    Even though muscle injuries are very common, few scientific data on their effective treatment exist. Growth Factors (GFs) may have a role in accelerating muscle repair processes and a currently available strategy for their delivery into the lesion site is the use of autologous platelet-rich plasma (PRP). The present study is focused on the use of Platelet Rich Fibrin Matrix (PRFM), as a source of GFs. Bilateral muscular lesions were created on the longissimus dorsi muscle of Wistar rats. One side of the lesion was filled with a PRFM while the contralateral was left untreated (controls). Animals were sacrificed at 5, 10, 40 and 60 days from surgery. Histological, immunohistochemical and histomorphometric analyses were performed to evaluate muscle regeneration, neovascularization, fibrosis and inflammation. The presence of metaplasia zones, calcifications and heterotopic ossification were also assessed. PRFM treated muscles exhibited an improved muscular regeneration, an increase in neovascularization, and a slight reduction of fibrosis compared with controls. No differences were detected for inflammation. Metaplasia, ossification and heterotopic calcification were not detected. This preliminary morphological experimental study shows that PRFM use can improve muscle regeneration and long-term vascularization. Since autologous blood products are safe, PRFM may be a useful and handy product in clinical treatment of muscle injuries.

  9. Management of pulpal floor perforation and grade II Furcation involvement using mineral trioxide aggregate and platelet rich fibrin: A clinical report

    Directory of Open Access Journals (Sweden)

    Rhythm Bains

    2012-01-01

    Full Text Available To report the management of an iatrogenic perforation of pulpal floor in the furcation of mandibular first molar, using Mineral Trioxide Aggregate (MTA and platelet rich fibrin (PRF. Unpredictable endodontic root/pulp chamber floor perforations resulting in unacceptable high rate of clinical failure has now been a lesser threat with the advent of new technologies and biocompatible materials that utilize the applications of basic research along with tissue engineering concept in clinical practice. Present case report illustrates the use of MTA and platelet rich fibrin (PRF for the repair of the perforation defect and regeneration of the lost periodontium in furcation area. Although, histologic events and reaction of MTA with PRF is not studied so far, however, the autologous and biocompatible nature of the components used for present treatment modalities seems to be beneficial for the long term clinical results obtained in our case.

  10. Trust as Glue in Nanotechnology Governance Networks.

    Science.gov (United States)

    Am, Heidrun

    2011-04-01

    This paper reflects on the change of relations among participants in nanotechnology governance through their participation in governance processes such as stakeholder dialogues. I show that policymaking in practice-that is, the practice of coming and working together in such stakeholder dialogues-has the potential for two-fold performative effects: it can contribute to the development of trust and mutual responsibility on the part of the involved actors, and it may bring about effects on the formation of boundaries of what is sayable and thinkable in nanotechnology governance. Three vignettes about the work of the German NanoKommission indicate the development of new relations of trust, recognition and mutual responsibility among actors. It is concluded that governance in practice can assemble new collectives in which relations of trust are the glue holding the complex structure together. While such a consensus-based progress may be favourable for smooth technology development, it can be considered problematic if evaluated against the ideals of deliberative democracy, which often form the premises on which public engagement is based. Stakeholder forums were set in place with the intention of including various actors, but this is Janus-faced: if a dialogue becomes encapsulated in new governance networks, new exclusions can arise. For example, a policing of which information is released to a wider audience can occur.

  11. Perivenous application of fibrin glue reduces early injury to the human saphenous vein graft wall in an ex vivo model

    NARCIS (Netherlands)

    Stooker, W; Niessen, HWM; Wildevuur, WR; van Hinsbergh, VWM; Fritz, J; Jansen, EK; Wildevuur, CRH; Eijsman, L

    2002-01-01

    Objectives: From animal and clinical studies it is known that prevention of 'overdistention' of vein grafts by using extravascular support ameliorates the arterialization process in vein grafts with subsequent more favorable patency. The most ideal support is a biodegradable, porous, elastic graft (

  12. Glue spin and helicity in proton from lattice QCD

    CERN Document Server

    Yang, Yi-Bo; Alexandru, Andrei; Draper, Terrence; Glatzmaier, Michael J; Liu, Keh-Fei; Zhao, Yong

    2016-01-01

    We report the first lattice QCD calculation of the glue spin $S_G$ in the nucleon. The lattice calculation is carried out with valence overlap fermions on 2+1 flavor DWF gauge configurations on four lattice spacings and four volumes including an ensemble with physical values for the quark masses. The glue spin $S_G$ in the $\\overline{\\text{MS}}$ scheme is obtained with the 1-loop perturbative matching. We find the results to be fairly insensitive to lattice spacing and quark masses. Since the frame dependence in the kinematic range $0\\leq \\vec{p} \\leq 1.5$ GeV is very mild, we take the first order large momentum expansion correction and determine the glue spin at the large momentum limit to be $S_G$=0.287(55)(16) at the physical pion mass in the $\\overline{\\text{MS}}$ scheme at $\\mu^2=10$ GeV$^2$. If the matching effect between the glue spin and helicity can be neglected, the glue helicity will be the same as the above value.

  13. A Bone Glue with Sustained Adhesion under Wet Conditions.

    Science.gov (United States)

    Wistlich, Laura; Rücker, Anja; Schamel, Martha; Kübler, Alexander C; Gbureck, Uwe; Groll, Jürgen

    2017-02-01

    Bone glues often suffer from low adhesion to bone under wet conditions. This study aims to improve wet adhesiveness of a bone glue based on a photocurable poly(ethylene glycol) dimethacrylate matrix through in situ interpenetrating network formation by addition of six-armed isocyanate functional star-shaped prepolymers (NCO-sP(EO-stat-PO)). Biodegradable ceramic fillers are added to adjust the paste workability. The 3-point bending strength of the bone glues is in the range of 3.5-5.5 MPa and not significantly affected by the addition of NCO-sP(EO-stat-PO). Storage in phosphate buffered saline (PBS) decreases the bending strength of all formulations to approximately 1 MPa but the adhesion to cortical bone increases from 0.15-0.2 to 0.3-0.5 MPa after adding 20-40 wt% NCO-sP(EO-stat-PO) to the matrix. Bone glues without the NCO-sP(EO-stat-PO) additive lose their adhesiveness to bone after aging in PBS for 7 days, whereas modified glues maintain a shear strength of 0.18-0.25 MPa demonstrating the efficacy of the approach. Scanning electron microscopy and energy-dispersive X-ray spectroscopy investigations of the fracture surfaces prove a high amount of residual adhesive on the bone surface indicating that adhesion to the bone under wet conditions is stronger than cohesion.

  14. Autologous Fat Injection for Augmented Mammoplasty

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Eul Sik; Seo, Bo Kyoung; Yi, Ann; Cho, Kyu Ran [Korea University Ansan Hospital, Ansan (Korea, Republic of)

    2008-12-15

    Autologous fat injection is one of the methods utilized for augmented mammoplasty methods. In this surgical procedure, the fat for transfer is obtained from the donor site of the patient's own body by liposuction and the fat is then injected into the breast. We report here cases of three patients who underwent autologous fat injection. Two of the patients had palpable masses that were present after surgery. The serial imaging findings and surgical method of autologous fat transfer are demonstrated

  15. Information-provider scripts for GLUE2 and RAID configurations.

    CERN Document Server

    Kalimeris, Dimitrios

    2014-01-01

    This report will outline two projects that were done as part of a three months long summer internship at CERN. In the first project we dealt with Worldwide LHC Computing Grid (WLCG) and its information system. The information system currently conforms to a schema called GLUE and it is evolving towards a new version: GLUE2. The aim of the project was to develop and adapt the current information system of the WLCG, used by the Large Scale Storage Systems at CERN (CASTOR and EOS), to the new GLUE2 schema. During the second project we investigated different RAID configurations so that we can get performance boost from CERN's disk systems in the future. RAID 1 that is currently in use is not an option anymore because of limited performance and high cost. We tried to discover RAID configurations that will improve the performance and simultaneously decrease the cost.

  16. Identification of fibrin clot-bound plasma proteins

    NARCIS (Netherlands)

    S. Talens (Simone); F.W.G. Leebeek (Frank); J.A.A. Demmers (Jeroen); D.C. Rijken (Dingeman)

    2012-01-01

    textabstractSeveral proteins are known to bind to a fibrin network and to change clot properties or function. In this study we aimed to get an overview of fibrin clot-bound plasma proteins. A plasma clot was formed by adding thrombin, CaCl2 and aprotinin to citrated platelet-poor plasma and unbound

  17. [Autologous fat grafting in children].

    Science.gov (United States)

    Baptista, C; Bertrand, B; Philandrianos, C; Degardin, N; Casanova, D

    2016-10-01

    Lipofilling or fat grafting transfer is defined as a technique of filling soft tissue by autologous fat grafting. The basic principle of lipofilling is based on a harvest of adipose tissue, followed by a reinjection after treatment. Lipofilling main objective is a volume defect filling, but also improving cutaneous trophicity. Lipofilling specificities among children is mainly based on these indications. Complications of autologous fat grafting among children are the same as those in adults: we distinguish short-term complications (intraoperative and perioperative) and the medium and long-term complications. The harvesting of fat tissue is the main limiting factor of the technique, due to low percentage of body fat of children. Indications of lipofilling among children may be specific or similar to those in adults. There are two types of indications: cosmetic, in which the aim of lipofilling is correcting a defect density, acquired (iatrogenic, post-traumatic scar) or malformation (otomandibular dysplasia, craniosynostosis, Parry Romberg syndrom, Poland syndrom, pectus excavatum…). The aim of functional indications is correcting a velar insufficiency or lagophthalmos. In the paediatric sector, lipofilling has become an alternative to the conventional techniques, by its reliability, safety, reproducibility, and good results.

  18. Tests of optical glues for the PANDA electromagnetic calorimeter

    NARCIS (Netherlands)

    Dbeyssi, A.; Tomasi-Gustafsson, E.; Hennino, T.; Imre, M.; Kunne, R.; Le Galliard, C.; Marchand, D.; Maroni, A.; Ramstein, B.; Rosier, P.; Bremer, D.; Dormenev, V.; Eissner, T.; Kuske, T.; Novotny, R.; Moeini, H.; Bondarenko, O.; Kavatsyuk, M.; Loehner, H.; Messchendorp, G.; Tambave, G.

    2013-01-01

    This paper reports on the results of tests for low temperature applications of two commercial optical glues in the electromagnetic calorimeter of PANDA at FAIR. Mechanical, thermal and optical properties are presented, as well as radiation hardness to photon and proton radiation. (C) 2013 Elsevier B

  19. Radiation Hardness of Some Polymers and Epoxy Glues

    CERN Document Server

    Gladkikh, S N; Kekelidze, G D; Mishin, S V; Peshekhonov, V D

    2001-01-01

    The radiation hardness of polysulphone and polycarbonate and also epoxy glues ELK-5 and CMK-5 was studied. This organic materials are being used for production of coordinate detectors. With that end in view dependences of maximum and maximum permissible loads and Brinell hardness on the absorbed doze for polymers were studied. For glues dependences of breaking point and relative lengthening on the stretching were studied. Irradiations were carried out on the experimental reactors providing a stream of fast neutrons with an energy spectrum in a range 0.1-20 MeV. Average energy of a \\gamma-component of a beam was 1.5-2 MeV and maximal one was up to 9 MeV. The maximal absorbed doze for glues was \\sim 100 kGy and for polymers was \\sim 1000 kGy. Results of measurements have shown weak dependence of mechanical properties of the investigated polymers and glues on the absorbed doze that allows to use them for detectors production within the framework of LHC project.

  20. Tests of optical glues for the PANDA electromagnetic calorimeter

    Energy Technology Data Exchange (ETDEWEB)

    Dbeyssi, A. [Univ Paris-Sud, CNRS/IN2P3, Institut de Physique Nucléaire, UMR 8608, 91405 Orsay (France); Tomasi-Gustafsson, E., E-mail: egle.tomasi@cea.fr [Univ Paris-Sud, CNRS/IN2P3, Institut de Physique Nucléaire, UMR 8608, 91405 Orsay (France); Hennino, T.; Imre, M.; Kunne, R.; Le Galliard, C.; Marchand, D.; Maroni, A.; Ramstein, B.; Rosier, P. [Univ Paris-Sud, CNRS/IN2P3, Institut de Physique Nucléaire, UMR 8608, 91405 Orsay (France); Bremer, D.; Dormenev, V.; Eissner, T.; Kuske, T.; Novotny, R. [Physikalishes Institut II, Universität Giessen (Germany); Moeini, H.; Bondarenko, O.; Kavatsyuk, M.; Loehner, H.; Messchendorp, J.G. [Kernfysisch Versneller Institute (KVI), University of Groningen, Groningen (Netherlands); and others

    2013-09-11

    This paper reports on the results of tests for low temperature applications of two commercial optical glues in the electromagnetic calorimeter of PANDA at FAIR. Mechanical, thermal and optical properties are presented, as well as radiation hardness to photon and proton radiation.

  1. Revascularization Induced Maturogenesis of Non-Vital Immature Permanent Tooth Using Platelet-Rich-Fibrin: A Case Report.

    Science.gov (United States)

    Nagaveni, N B; Pathak, Sidhant; Poornima, P; Joshi, Jooie S

    2016-01-01

    The aim of this report is to describe a novel method of revascularization therapy done in a non-vital, immature permanent tooth using Platelet-rich fibrin (PRF),in a recently developed scaffold material to overcome limitations associated with the traditional method of revascularization using natural blood clot. PRF prepared from autologous blood was placed in the root canal and patient was followed up regularly at one, three, six, nine and 12 months for detailed clinical and radiographic evaluation. At 12 months, radiographic examination revealed root elongation, root end closure, continued thickening of the root dentinal walls, obliteration of root canal space, and normal periradicular anatomy. However, more long term prospective trials and histological studies are highly needed before to testify PRF a panacea for the regenerative endodontic therapy in children.

  2. Laterally positioned flap-revised technique along with platelet rich fibrin in the management of Miller class II gingival recession

    Directory of Open Access Journals (Sweden)

    Jagmohan Singh

    2013-01-01

    Full Text Available Gingival recession is a complex phenomenon that may present numerous therapeutic challenges to the clinician. The laterally positioned flap is commonly used to cover isolated, denuded roots that have adequate donor tissue laterally and vestibular depth. Various modifications in laterally sliding flap have been proposed in order to avoid the reported undesirable results on the donor teeth. Recently, use of growth factors has been proposed in combination with surgical techniques. This article highlights the use of laterally positioned pedicle flap-revised technique as a modification of laterally sliding flap technique along with autologous suspension of growth factors, platelet rich fibrin membrane (PRF for the management of localized Miller class-II gingival recession. After 6 months of follow-up, the clinical condition was stable with 80% root coverage and satisfactory gingival tissue healing at both donor and recipient site with no signs of inflammation. An excellent esthetical outcome was achieved and the patient was satisfied with case resolution.

  3. Release kinetics of platelet-derived and plasma-derived growth factors from autologous plasma rich in growth factors.

    Science.gov (United States)

    Anitua, Eduardo; Zalduendo, Mari Mar; Alkhraisat, Mohammad Hamdan; Orive, Gorka

    2013-10-01

    Many studies have evaluated the biological effects of platelet rich plasma reporting the final outcomes on cell and tissues. However, few studies have dealt with the kinetics of growth factor delivery by plasma rich in growth factors. Venous blood was obtained from three healthy volunteers and processed with PRGF-Endoret technology to prepare autologous plasma rich in growth factors. The gel-like fibrin scaffolds were then incubated in triplicate, in a cell culture medium to monitor the release of PDGF-AB, VEGF, HGF and IGF-I during 8 days of incubation. A leukocyte-platelet rich plasma was prepared employing the same technology and the concentrations of growth factors and interleukin-1β were determined after 24h of incubation. After each period, the medium was collected, fibrin clot was destroyed and the supernatants were stored at -80°C until analysis. The growth factor delivery is diffusion controlled with a rapid initial release by 30% of the bioactive content after 1h of incubation and a steady state release when almost 70% of the growth factor content has been delivered. Autologous fibrin matrix retained almost 30% of the amount of the growth factors after 8 days of incubation. The addition of leukocytes to the formula of platelet rich plasma did not increase the concentration of the growth factors, while it drastically increased the presence of pro-inflammatory IL-1β. Further studies employing an in vitro inflammatory model would be interesting to study the difference in growth factors and pro-inflammatory cytokines between leukocyte-free and leukocyte-rich platelet rich plasma.

  4. Predictive factors for anterior chamber fibrin formation after vitreoretinal surgery

    Directory of Open Access Journals (Sweden)

    Leonardo Provetti Cunha

    2014-04-01

    Full Text Available Purpose: The aim of this study was to investigate possible predictive factors related to anterior chamber fibrin formation after vitreoretinal surgery in a large series of patients. Methods: The data of 185 eyes of 185 patients submitted to vitreoretinal surgery was reviewed. The following variables were evaluated: the postoperatively presence of fibrin, age, diabetes mellitus, the vitrectomy system gauge (20, 23 or 25 gauge, the type of vitreous substitute, the influence of prior surgical procedures and the combination with cataract extraction. To evaluate predictive factors for anterior chamber fibrin formation, univariate analysis was performed. A multivariate stepwise logistic regression model was adjusted to investigate factors associated with fibrin formation (p<0.05. Results: Fibrinoid anterior chamber reaction was found in 12 (6.4% patients. For multivariate logistic regression analysis, balanced salt solution (BSS, the chance of fibrin occurrence was 5 times greater (odds ratio 4.83, CI 95% 1.302 - 17.892; p=0.019, while combination with phacoemulsification increased the chance of fibrin formation by 20 times (odds ratio 20, CI 95% 2.480 - 161.347; p=0.005. No significant difference was found regarding other variables. Conclusion: Anterior chamber fibrin formation is an unwanted complication after vitreoretinal surgery. Factors such as combined performance of phacoemulsification and the use of balanced salt solution as a vitreous substitute may predispose the occurrence of this complication.

  5. Tumour imaging by the detection of fibrin clots in tumour stroma using an anti-fibrin Fab fragment.

    Science.gov (United States)

    Obonai, Toshifumi; Fuchigami, Hirobumi; Furuya, Fumiaki; Kozuka, Naoyuki; Yasunaga, Masahiro; Matsumura, Yasuhiro

    2016-01-01

    The diagnosis of early and aggressive types of cancer is important for providing effective cancer therapy. Cancer-induced fibrin clots exist only within lesions. Previously, we developed a monoclonal antibody (clone 102-10) that recognizes insoluble fibrin but not fibrinogen or soluble fibrin and confirmed that fibrin clots form continuously in various cancers. Here, we describe the development of a Fab fragment probe of clone 102-10 for tumour imaging. The distribution of 102-10 Fab was investigated in genetically engineered mice bearing pancreatic ductal adenocarcinoma (PDAC), and its effect on blood coagulation was examined. Immunohistochemical and ex vivo imaging revealed that 102-10 Fab was distributed selectively in fibrin clots in PDAC tumours 3 h after injection and that it disappeared from the body after 24 h. 102-10 Fab had no influence on blood coagulation or fibrinolysis. Tumour imaging using anti-fibrin Fab may provide a safe and effective method for the diagnosis of invasive cancers by detecting fibrin clots in tumour stroma.

  6. Management of radicular cysts using platelet-rich fibrin and bioactive glass: a report of two cases.

    Science.gov (United States)

    Zhao, Jiing-Huei; Tsai, Chung-Hung; Chang, Yu-Chao

    2014-07-01

    Platelet-rich fibrin (PRF) created by Choukroun's protocol concentrates most platelets and leukocytes from a blood harvest into a single autologous fibrin biomaterial. However, no current data is available concerning the use of PRF for the treatment of periapical lesions. Two cases of radicular cysts were reported using an interdisciplinary approach, including regular endodontic therapy followed by surgical management with PRF and bioactive glass. Two cases of radicular cysts presented as an incidental radiographic finding, appearing as an apical radiolucency with well-circumscribed sclerotic borders. After regular endodontic retreatment, cystic lining/granulation tissues were enucleated and the periradicular bony defect was grafted using PRF and bioactive glass. Then, PRF was applied to serve as a membrane over the grafted defects. Recall periapical radiographs of Case 1 and cone beam computer tomography of Case 2 showed satisfactory healing of the periapical pathosis. In Case 2, the bony defect appeared completely healed at 4 months surgical reentry and the new bone was clinically very dense and mature. The results of these case reports show that the combination of PRF and bioactive glass is an effective modality of regenerative treatment for radicular cysts.

  7. Bone neoformation in defects treated with platelet-rich fibrin membrane versus collagen membrane: a histomorphometric study in rabbit femurs.

    Directory of Open Access Journals (Sweden)

    Edwin Meza

    2015-02-01

    Full Text Available The aim of the present research was to compare bone neoformation in bone defects treated with platelet-rich fibrin (PRF and collagen membrane (CM at 3 and 5 weeks. For this purpose, two bone defects with a width of 4 mm and depth of 6 mm were created in the left distal femur diaphysis of New Zealand rabbits (n=12. The subjects were randomly allocated into two groups. One of the defects was covered with a platelet-rich fibrin membrane (Centrifuged resorbable autologous blood biopolymer without biochemical modification or a collagen membrane (gold standard - Neo Mem. The second defect was left uncovered (NC. The rabbits were sacrificed after 3 and 5 weeks (3 rabbits per period. The femur was completely removed and processed histomophometrically. The bone neformation analysis was performed using a differential point-counting method. Data was statistically analyzed (ANOVA, Tukey. The histomorphometric results showed that bone neformation in the defects treated with PRF at 3 weeks was equivalent to the CM (p<0.05. After 5 weeks, bone neformation obtained with PRF was higher than the control group and lower compared with the CM (p<0.05. The conclusion of the present study is that bone neformation in defects treated with PRF showed lower histomorphometric results compared with the one obtained with the collagen membrane and higher when compared with the control defects.

  8. Screening for autologous blood transfusions

    DEFF Research Database (Denmark)

    Mørkeberg, J; Belhage, B; Ashenden, M

    2009-01-01

    The ratio between the amount of hemoglobin in the mature erythrocyte population and the reticulocytes (RBCHb:RetHb ratio) has previously been suggested as a marker to screen for EPO-abuse. We speculated that the reinfusion of blood would lead to a marked increase in this ratio, making it a valuab...... doping after reinfusion, and the parameter could be used in a testing setting, once stability validation has been performed....... parameter in the screening for autologous blood doping. Three bags of blood (approximately 201+/-11 g of Hb) were withdrawn from 16 males and stored at either -80 degrees C (-80 T, n=8) or +4 degrees C (+4 T, n=8) and reinfused 10 weeks or 4 weeks later, respectively. Seven subjects served as controls...

  9. Altered plasma fibrin clot properties in essential thrombocythemia.

    Science.gov (United States)

    Małecki, Rafał; Gacka, Małgorzata; Kuliszkiewicz-Janus, Małgorzata; Jakobsche-Policht, Urszula; Kwiatkowski, Jacek; Adamiec, Rajmund; Undas, Anetta

    2016-01-01

    Patients with increased thromboembolic risk tend to form denser fibrin clots which are relatively resistant to lysis. We sought to investigate whether essential thrombocythemia (ET) is associated with altered fibrin clot properties in plasma. Ex vivo plasma fibrin clot permeability coefficient (Ks), turbidimetry and clot lysis time (CLT) were measured in 43 consecutive patients with ET (platelet count from 245 to 991 × 10(3)/µL) and 50 control subjects matched for age, sex and comorbidities. Fibrinolysis proteins and inhibitors together with platelet activation markers were determined. Reduced Ks (-38%, p Ks inversely correlated with fibrinogen, PF4 and C-reactive protein. CLT positively correlated only with PAI-1. Patients with ET display prothrombotic plasma fibrin clot phenotype including impaired fibrinolysis, which represents a new prothrombotic mechanism in this disease.

  10. Intraurethral Injection of Autologous Minced Skeletal Muscle

    DEFF Research Database (Denmark)

    Gräs, Søren; Klarskov, Niels; Lose, Gunnar

    2014-01-01

    PURPOSE: Intraurethral injection of in vitro expanded autologous skeletal muscle derived cells is a new regenerative therapy for stress urinary incontinence. We examined the efficacy and safety of a simpler alternative strategy using freshly harvested, minced autologous skeletal muscle tissue...... with its inherent content of regenerative cells. MATERIALS AND METHODS: A total of 20 and 15 women with uncomplicated and complicated stress urinary incontinence, respectively, received intraurethral injections of minced autologous skeletal muscle tissue and were followed for 1 year. Efficacy was assessed...... noted. CONCLUSIONS: Intraurethral injection of minced autologous muscle tissue is a simple surgical procedure that appears safe and moderately effective in women with uncomplicated stress urinary incontinence. It compares well to a more complicated regenerative strategy using in vitro expanded muscle...

  11. Autologous serum therapy in chronic urticaria

    Directory of Open Access Journals (Sweden)

    Sharmila Patil

    2013-01-01

    Full Text Available Autologous serum therapy is a promising therapy for treatment resistant urticaria. This is useful in developing countries as this is economical option. Minimum instruments like centrifuge, syringe and needles are required for the procedure.

  12. Fibrin sealant use in pilonidal sinus: Systematic review

    Institute of Scientific and Technical Information of China (English)

    Cuneyt Kayaalp; Ismail Ertugrul; Kerem Tolan; Fatih Sumer

    2016-01-01

    AIM: To review the current data about the success rates of fibrin sealant use in pilonidal disease.METHODS: Fibrin sealant can be used for different purposes in pilonidal sinus treatment, such as filling in the sinus tracts, covering the open wound after excision and lay-open treatment, or obliterating the subcutaneous dead space before skin closure. We searched Pubmed, Google-Scholar, Ebsco-Host, clinicaltrials, and Cochrane databases and found nine studies eligible for analysis; these studies included a total of 217 patients(84% male, mean age 24.2 ± 7.8). RESULTS: In cases where fibrin sealant was used to obliterate the subcutaneous dead space, there was no reduction in wound complication rates(9.8% vs 14.6%, P = 0.48). In cases where sealant was used to cover the laid-open area, the wound healing time and patient comfort were reported better than in previous studies(mean 17 d, 88% satisfaction). When fibrin sealant was used to fill the sinus tracts, the recurrence rate was around 20%, despite the highly selected grouping of patients.CONCLUSION: Consequently, using fibrin sealant to decrease the risk of seroma formation was determined to be an ineffective course of action. It was not advisable to fill the sinus tracts with fibrin sealant because it was not superior to other cost-effective and minimally invasive treatments. New comparative studies can be conducted to confirm the results of sealant use in covering the laid-open area.

  13. Multivalent viral capsids with internal cargo for fibrin imaging.

    Directory of Open Access Journals (Sweden)

    Allie C Obermeyer

    Full Text Available Thrombosis is the cause of many cardiovascular syndromes and is a significant contributor to life-threatening diseases, such as myocardial infarction and stroke. Thrombus targeted imaging agents have the capability to provide molecular information about pathological clots, potentially improving detection, risk stratification, and therapy of thrombosis-related diseases. Nanocarriers are a promising platform for the development of molecular imaging agents as they can be modified to have external targeting ligands and internal functional cargo. In this work, we report the synthesis and use of chemically functionalized bacteriophage MS2 capsids as biomolecule-based nanoparticles for fibrin imaging. The capsids were modified using an oxidative coupling reaction, conjugating ∼90 copies of a fibrin targeting peptide to the exterior of each protein shell. The ability of the multivalent, targeted capsids to bind fibrin was first demonstrated by determining the impact on thrombin-mediated clot formation. The modified capsids out-performed the free peptides and were shown to inhibit clot formation at effective concentrations over ten-fold lower than the monomeric peptide alone. The installation of near-infrared fluorophores on the interior surface of the capsids enabled optical detection of binding to fibrin clots. The targeted capsids bound to fibrin, exhibiting higher signal-to-background than control, non-targeted MS2-based nanoagents. The in vitro assessment of the capsids suggests that fibrin-targeted MS2 capsids could be used as delivery agents to thrombi for diagnostic or therapeutic applications.

  14. The Forward Calorimeter of the GlueX Experiment

    Science.gov (United States)

    Bennett, Daniel; GlueX Collaboration

    2013-10-01

    The Forward Calorimeter (FCAL) of the GlueX experiment is a lead glass electromagnetic calorimeter currently being built in Hall D of Jefferson Lab. The GlueX experiment is a photoproduction experiment that will utilize coherent bremsstrahlung radiation to map out the light meson spectrum, including a search for hybrid mesons with exotic quantum numbers (JPC). The FCAL will detect photons between 1° and 10 .8° downstream from the target. The calorimeter is built out of 2800 elements, each of which consists of a lead glass block, an FEU 84-3 PMT, and a custom Cockcroft-Walton electronic base. In the Fall of 2011, a 25 element prototype detector was installed in Hall B of Jefferson Lab to measure the energy and timing resolution of the calorimeter using electrons between 100 and 250 MeV. The design and construction of FCAL and the results from the prototype test will be discussed.

  15. Glasses, Coatings, Glues and Gamma-ray Irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Barcala, J.M.; Fernandez, M. G.; Ferrando, A.; Fuentes, J.; Josa, M. I.; Molinero, A.; Oller, J. C. [Ciemat. Madrid (Spain); Arce, P.; Calvo, E.; Figueroa, C. F.; Rodrigo, T.; Vila, I.; Virto, A. L. [Universidad de Cantabria. Santander (Spain); Beigveder, J. M.; Genova, I.; Perez, G.; Ruiz, J. A. [CIDA. Madrid (Spain)

    2001-07-01

    Most of the alignment systems for LHC experiments use optomechanical elements confirming a network of points that are monitored by laser beams. LHC experiments, working at the expected nominal luminosity, will induce an extremely high irradiation. basic components such as glasses, coatings and glues may change and their performance may degrade significantly. We have tested various components and identified some of them that can stand 10 years of LHC operation. (Author) 11 refs.

  16. Activated Schwann Cell-Like Cells on Aligned Fibrin-Poly(Lactic-Co-Glycolic Acid) Structures: A Novel Construct for Application in Peripheral Nerve Regeneration.

    Science.gov (United States)

    Schuh, Christina M A P; Morton, Tatjana J; Banerjee, Asmita; Grasl, Christian; Schima, Heinrich; Schmidhammer, Robert; Redl, Heinz; Ruenzler, Dominik

    2015-01-01

    Tissue engineering approaches in nerve regeneration search for ways to support gold standard therapy (autologous nerve grafts) and to improve results by bridging nerve defects with different kinds of conduits. In this study, we describe electrospinning of aligned fibrin-poly(lactic-co-glycolic acid) (PLGA) fibers in an attempt to create a biomimicking tissue-like material seeded with Schwann cell-like cells (SCLs) in vitro for potential use as an in vivo scaffold. Rat adipose-derived stem cells (rASCs) were differentiated into SCLs and evaluated with flow cytometry concerning their differentiation and activation status [S100b, P75, myelin-associated glycoprotein (MAG), and protein 0 (P0)]. After receiving the proliferation stimulus forskolin, SCLs expressed S100b and P75; comparable to native, activated Schwann cells, while cultured without forskolin, cells switched to a promyelinating phenotype and expressed S100b, MAG, and P0. Human fibrinogen and thrombin, blended with PLGA, were electrospun and the alignment and homogeneity of the fibers were proven by scanning electron microscopy. Electrospun scaffolds were seeded with SCLs and the formation of Büngner-like structures in SCLs was evaluated with phalloidin/propidium iodide staining. Carrier fibrin gels containing rASCs acted as a self-shaping matrix to form a tubular structure. In this study, we could show that rASCs can be differentiated into activated, proliferating SCLs and that these cells react to minimal changes in stimulus, switching to a promyelinating phenotype. Aligned electrospun fibrin-PLGA fibers promoted the formation of Büngner-like structures in SCLs, which also rolled the fibrin-PLGA matrix into a tubular scaffold. These in vitro findings favor further in vivo testing.

  17. Infiltrated Embolization of Meningioma with Dilute Cyanoacrylate Glue

    Science.gov (United States)

    OHNISHI, Hiroyuki; MIYACHI, Shigeru; MURAO, Kenichi; HIRAMATSU, Ryo; TAKAHASHI, Kenkichi; OHNISHI, Hideyuki; KUROIWA, Toshihiko

    2017-01-01

    We describe the efficacy and technical aspects of infiltrated preoperative embolization of meningioma by penetration of very dilute glue. In this method, a 13% n-butyl-cyanoacrylate (NBCA)-lipiodol mixture is injected extremely slowly from the middle meningeal artery (MMA) in a similar manner to plug and push injection of ethylene vinyl alcohol copolymer mixed with tantalum and dimethyl sulfoxide (Onyx®) after the tortuous side feeders are proximally embolized. The glue is infiltrated into small tumor arteries and extends to inaccessible feeders from deep meningeal arteries. Since 2011, we have used this technique in the embolization of 32 cases preoperatively diagnosed with meningioma. Intratumoral embolization was possible in 30 cases (94%), and a greater than 50% reduction in contrast area of contrast-enhanced T1-weighted MR imaging (T1-WI) was achieved in 18 cases (56%). Two cases achieved complete devascularization, showing a remarkable shrinkage in tumor size after embolization. If excessive reflux of embolization and the resulting migration of glue into normal arteries is achieved, this method provides extremely effective devascularization on surgical extirpation. It might also be applicable to surgically untreatable meningiomas as a semi-radical treatment option. PMID:27646010

  18. Cell-seeded polyurethane-fibrin structures – A possible system for intervertebral disc regeneration

    Directory of Open Access Journals (Sweden)

    C Mauth

    2009-10-01

    Full Text Available Nowadays, intervertebral disc (IVD degeneration is one of the principal causes of low back pain involving high expense within the health care system. The long-term goal is the development of a medical treatment modality focused on a more biological regeneration of the inner nucleus pulposus (NP. Hence, interest in the endoscopic implantation of an injectable material took center stage in the recent past. We report on the development of a novel polyurethane (PU scaffold as a mechanically stable carrier system for the reimplantation of expanded autologous IVD-derived cells (disc cells to stimulate regenerative processes and restore the chondrocyte-like tissue within the NP. Primary human disc cells were seeded into newly developed PU spheroids which were subsequently encapsulated in fibrin hydrogel. The study aims to analyze adhesion properties, proliferation capacity and phenotypic characterization of these cells. Polymerase chain reaction was carried out to detect the expression of genes specifically expressed by native IVD cells. Biochemical analyses showed an increased DNA content, and a progressive enhancement of total collagen and glycosaminoglycans (GAG was observed during cell culture. The results suggest the synthesis of an appropriate extracellular matrix as well as a stable mRNA expression of chondrogenic and/or NP specific markers. In conclusion, the data presented indicate an alternative medical approach to current treatment options of degenerated IVD tissue.

  19. A Novel Technique for Conjunctivoplasty in a Rabbit Model: Platelet-Rich Fibrin Membrane Grafting

    Science.gov (United States)

    Çakmak, Hasan Basri; Dereli Can, Gamze; Ünverdi, Hatice; Toklu, Yasin; Hücemenoğlu, Sema

    2016-01-01

    Purpose. To investigate the effect of platelet-rich fibrin (PRF) membrane on wound healing. Methods. Twenty-four right eyes of 24 New Zealand rabbits equally divided into 2 groups for the study design. After the creation of 5 × 5 mm conjunctival damage, it was secured with PRF membrane, which was generated from the rabbit's whole blood samples in PRF membrane group, whereas damage was left unsutured in the control group. Three animals were sacrificed in each group on the 1st, 3rd, 7th, and 28th postoperative days. Immunohistochemical (IHC) stainings and biomicroscopic evaluation were performed and compared between groups. Results. PRF membrane generated significant expressions of vascular endothelial growth factor (VEGF), transforming growth factor-beta (TGF-β), and platelet-derived growth factor (PDGF) in the early postoperative period. However, the IHC evaluation allowed showing the excessive staining at day 28, in control group. Biomicroscopic evaluation revealed complete epithelialization in PRF membrane group, but none of the cases showed complete healing in the control group. Conclusions. This experimental study showed us the beneficial effects of the PRF membrane on conjunctival healing. Besides its chemical effects, it provides mechanical support as a scaffold for the migrating cells that are important for ocular surface regeneration. These overall results encourage us to apply autologous PRF membrane as a growth factor-enriched endogenous scaffold for ocular surface reconstruction. PMID:27747098

  20. A Novel Technique for Conjunctivoplasty in a Rabbit Model: Platelet-Rich Fibrin Membrane Grafting

    Directory of Open Access Journals (Sweden)

    Mehmet Erol Can

    2016-01-01

    Full Text Available Purpose. To investigate the effect of platelet-rich fibrin (PRF membrane on wound healing. Methods. Twenty-four right eyes of 24 New Zealand rabbits equally divided into 2 groups for the study design. After the creation of 5 × 5 mm conjunctival damage, it was secured with PRF membrane, which was generated from the rabbit’s whole blood samples in PRF membrane group, whereas damage was left unsutured in the control group. Three animals were sacrificed in each group on the 1st, 3rd, 7th, and 28th postoperative days. Immunohistochemical (IHC stainings and biomicroscopic evaluation were performed and compared between groups. Results. PRF membrane generated significant expressions of vascular endothelial growth factor (VEGF, transforming growth factor-beta (TGF-β, and platelet-derived growth factor (PDGF in the early postoperative period. However, the IHC evaluation allowed showing the excessive staining at day 28, in control group. Biomicroscopic evaluation revealed complete epithelialization in PRF membrane group, but none of the cases showed complete healing in the control group. Conclusions. This experimental study showed us the beneficial effects of the PRF membrane on conjunctival healing. Besides its chemical effects, it provides mechanical support as a scaffold for the migrating cells that are important for ocular surface regeneration. These overall results encourage us to apply autologous PRF membrane as a growth factor-enriched endogenous scaffold for ocular surface reconstruction.

  1. Fibronectin alters the rate of formation and structure of the fibrin matrix.

    Science.gov (United States)

    Ramanathan, Anand; Karuri, Nancy

    2014-01-10

    Plasma fibronectin is a vital component of the fibrin clot; however its role on clot structure is not clearly understood. The goal of this study was to examine the influence of fibronectin on the kinetics of formation, structural characteristics and composition of reconstituted fibrin clots or fibrin matrices. Fibrin matrices were formed by adding thrombin to 1, 2 or 4 mg/ml fibrinogen supplemented with 0-0.4 mg/ml fibronectin. The rate of fibrin matrix formation was then monitored by measuring light absorbance properties at different time points. Confocal microscopy of fluorescein conjugated fibrinogen was used to visualize the structural characteristics of fibrin matrices. The amount of fibronectin in fibrin matrices was determined through electrophoresis and immunoblotting of solubilized matrices. Fibronectin concentration positively correlated with the initial rate of fibrin matrix formation and with steady state light absorbance values of fibrin matrices. An increase in fibronectin concentration resulted in thinner and denser fibers in the fibrin matrices. Electrophoresis and immunoblotting showed that fibronectin was covalently and non-covalently bound to fibrin matrices and in the form of high molecular weight multimers. The formation of fibronectin multimers was attributed to cross-linking of fibronectin by trace amounts Factor XIIIa. These findings are novel because they link results from light absorbance studies to microcopy analyses and demonstrate an influence of fibronectin on fibrin matrix structural characteristics. This data is important in developing therapies that destabilize fibrin clots.

  2. A CCR2 macrophage endocytic pathway mediates extravascular fibrin clearance in vivo

    Science.gov (United States)

    Motley, Michael P.; Madsen, Daniel H.; Jürgensen, Henrik J.; Spencer, David E.; Szabo, Roman; Holmbeck, Kenn; Flick, Matthew J.; Lawrence, Daniel A.; Castellino, Francis J.; Weigert, Roberto

    2016-01-01

    Extravascular fibrin deposition accompanies many human diseases and causes chronic inflammation and organ damage, unless removed in a timely manner. Here, we used intravital microscopy to investigate how fibrin is removed from extravascular space. Fibrin placed into the dermis of mice underwent cellular endocytosis and lysosomal targeting, revealing a novel intracellular pathway for extravascular fibrin degradation. A C-C chemokine receptor type 2 (CCR2)-positive macrophage subpopulation constituted the majority of fibrin-uptaking cells. Consequently, cellular fibrin uptake was diminished by elimination of CCR2-expressing cells. The CCR2-positive macrophage subtype was different from collagen-internalizing M2-like macrophages. Cellular fibrin uptake was strictly dependent on plasminogen and plasminogen activator. Surprisingly, however, fibrin endocytosis was unimpeded by the absence of the fibrin(ogen) receptors, αMβ2 and ICAM-1, the myeloid cell integrin-binding site on fibrin or the endocytic collagen receptor, the mannose receptor. The study identifies a novel fibrin endocytic pathway engaged in extravascular fibrin clearance and shows that interstitial fibrin and collagen are cleared by different subsets of macrophages employing distinct molecular pathways. PMID:26647393

  3. Failure mechanisms of fibrin-based surgical tissue adhesives

    Science.gov (United States)

    Sierra, David Hugh

    A series of studies was performed to investigate the potential impact of heterogeneity in the matrix of multiple-component fibrin-based tissue adhesives upon their mechanical and biomechanical properties both in vivo and in vitro. Investigations into the failure mechanisms by stereological techniques demonstrated that heterogeneity could be measured quantitatively and that the variation in heterogeneity could be altered both by the means of component mixing and delivery and by the formulation of the sealant. Ex vivo tensile adhesive strength was found to be inversely proportional to the amount of heterogeneity. In contrast, in vivo tensile wound-closure strength was found to be relatively unaffected by the degree of heterogeneity, while in vivo parenchymal organ hemostasis in rabbits was found to be affected: greater heterogeneity appeared to correlate with an increase in hemostasis time and amount of sealant necessary to effect hemostasis. Tensile testing of the bulk sealant showed that mechanical parameters were proportional to fibrin concentration and that the physical characteristics of the failure supported a ductile mechanism. Strain hardening as a function of percentage of strain, and strain rate was observed for both concentrations, and syneresis was observed at low strain rates for the lower fibrin concentration. Blister testing demonstrated that burst pressure and failure energy were proportional to fibrin concentration and decreased with increasing flow rate. Higher fibrin concentration demonstrated predominately compact morphology debonds with cohesive failure loci, demonstrating shear or viscous failure in a viscoelastic rubbery adhesive. The lower fibrin concentration sealant exhibited predominately fractal morphology debonds with cohesive failure loci, supporting an elastoviscous material condition. The failure mechanism for these was hypothesized and shown to be flow-induced ductile fracture. Based on these findings, the failure mechanism was

  4. Calix[4]arene methylenebisphosphonic acids as inhibitors of fibrin polymerization.

    Science.gov (United States)

    Lugovskoy, Eduard V; Gritsenko, Pavel G; Koshel, Tatyana A; Koliesnik, Ievgen O; Cherenok, Serhey O; Kalchenko, Olga I; Kalchenko, Vitaliy I; Komisarenko, Serhey V

    2011-04-01

    Calix[4]arenes bearing two or four methylenebisphosphonic acid groups at the macrocyclic upper rim have been studied with respect to their effects on fibrin polymerization. The most potent inhibitor proved to be calix[4]arene tetrakis-methylene-bis-phosphonic acid (C-192), in which case the maximum rate of fibrin polymerization in the fibrinogen + thrombin reaction decreased by 50% at concentrations of 0.52 × 10(-6) M (IC(50)). At this concentration, the molar ratio of the compound to fibrinogen was 1.7 : 1. For the case of desAABB fibrin polymerization, the IC(50) was 1.26 × 10(-6) M at a molar ratio of C-192 to fibrin monomer of 4 : 1. Dipropoxycalix[4]arene bis-methylene-bis-phosphonic acid (C-98) inhibited fibrin desAABB polymerization with an IC(50) = 1.31 × 10(-4) M. We hypothesized that C-192 blocks fibrin formation by combining with polymerization site 'A' (Aα17-19), which ordinarily initiates protofibril formation in a 'knob-hole' manner. This suggestion was confirmed by an HPLC assay, which showed a host-guest inclusion complex of C-192 with the synthetic peptide Gly-Pro-Arg-Pro, an analogue of site 'A'. Further confirmation that the inhibitor was acting at the initial step of the reaction was obtained by electron microscopy, with no evidence of protofibril formation being evident. Calixarene C-192 also doubled both the prothrombin time and the activated partial thromboplastin time in normal human blood plasma at concentrations of 7.13 × 10(-5) M and 1.10 × 10(-5) M, respectively. These experiments demonstrate that C-192 is a specific inhibitor of fibrin polymerization and blood coagulation and can be used for the design of a new class of antithrombotic agents.

  5. Ultrastructural changes to rabbit fibrin and platelets due to aspartame.

    Science.gov (United States)

    Pretorius, E; Humphries, P

    2007-01-01

    The coagulation process, including thrombin, fibrin, as well as platelets, plays an important role in hemostasis, contributing to the general well-being of humans. Fibrin formation and platelet activation are delicate processes that are under the control of many small physiological events. Any one of these many processes may be influenced or changed by external factors, including pharmaceutical or nutritional products, e.g., the sweetener aspartame (L-aspartyl-L-phenylalanine methyl ester). It is known that phenylalanine is present at position P(9) and aspartate at position P(10) of the alpha-chain of human fibrinogen, and plays an important role in the conversion of fibrinogen to fibrin by the catalyst alpha-thrombin. The authors investigate the effect of aspartame on platelet and fibrin ultrastructure, by using the rabbit animal model and the scanning electron microscope. Animals were exposed to 34 mg/kg of aspartame 26x during a 2-month period. Aspartame-exposed fibrin networks appeared denser, with a thick matted fine fiber network covering thick major fibers. Also, the platelet aggregates appeared more granular than the globular control platelet aggregates. The authors conclude by suggesting that aspartame usage may interfere with the coagulation process and might cause delayed fibrin breakup after clot formation. They suggest this, as the fibrin networks from aspartame-exposed rabbits are more complex and dense, due to the netlike appearance of the minor, thin fibers. Aspartame usage should possibly be limited by people on anti-clotting medicine or those with prone to clot formation.

  6. Identification of fibrin clot-bound plasma proteins.

    Directory of Open Access Journals (Sweden)

    Simone Talens

    Full Text Available Several proteins are known to bind to a fibrin network and to change clot properties or function. In this study we aimed to get an overview of fibrin clot-bound plasma proteins. A plasma clot was formed by adding thrombin, CaCl(2 and aprotinin to citrated platelet-poor plasma and unbound proteins were washed away with Tris-buffered saline. Non-covalently bound proteins were extracted, separated with 2D gel electrophoresis and visualized with Sypro Ruby. Excised protein spots were analyzed with mass spectrometry. The identity of the proteins was verified by checking the mass of the protein, and, if necessary, by Western blot analysis. Next to established fibrin-binding proteins we identified several novel fibrin clot-bound plasma proteins, including α(2-macroglobulin, carboxypeptidase N, α(1-antitrypsin, haptoglobin, serum amyloid P, and the apolipoproteins A-I, E, J, and A-IV. The latter six proteins are associated with high-density lipoprotein particles. In addition we showed that high-density lipoprotein associated proteins were also present in fibrinogen preparations purified from plasma. Most plasma proteins in a fibrin clot can be classified into three groups according to either blood coagulation, protease inhibition or high-density lipoprotein metabolism. The presence of high-density lipoprotein in clots might point to a role in hemostasis.

  7. Measurement of clottability of fibrin polymers using magnetic orientation

    Science.gov (United States)

    Iwasaka, M.; Ueno, S.; Tsuda, H.

    1996-04-01

    Fibrin polymers, as a kind of diamagnetic material, are oriented parallel to the direction of magnetic fields. We investigated the polymerization of partially digested fibrinogens in an 8 T magnetic field in order to observe the clotting ability of various sizes of fibrinogen fractions using a magnetic orientation technique. We purified high-molecular weight fraction (F1) and low-molecular weight fraction from human fibrinogen (F2). Fibrin gels were formed in an 8 T magnetic field for 9 h, and transmittancies were measured to evaluate the degree of magnetic orientation. The results show that a lack at the C-terminal half of one Aα chain did not affect the magnetic orientation of fibrin. We also investigated the effect of the digestion of fibrinogen by plasmin on the magnetic orientation of fibrin. The result shows that partially digested fibrin molecules also orient in an 8 T magnetic field. However, the degree of magnetic orientation significantly decreases when fragment X and fragment Y appear.

  8. Phase-separated chitosan-fibrin microbeads for cell delivery.

    Science.gov (United States)

    Chen, Zhewei; Wang, Limin; Stegemann, Jan P

    2011-01-01

    Matrix-enhanced delivery of cells is a promising approach to improving current cell therapies. Our objective was to create cell-laden composite microbeads that combine the attractive features of the natural polymers chitosan and fibrin. Liquid polydimethylsiloxane was used to emulsify a chitosan-fibrinogen solution containing suspended human fibroblast cells, followed by initiation of thrombin-mediated polymerization of fibrin and thermal/pH-mediated gelation of chitosan. Chitosan/fibrin weight percent (wt%) ratios of 100/0, 75/25, 50/50 and 25/75 were investigated. Microbead diameters ranged from 275 ± 99 µm to 38 ± 10 µm using impeller speeds from 600 to 1400 rpm. Fibroblasts remained viable on day 1 post-fabrication in all matrices, but cell viability was markedly higher in high-fibrin microbeads by day 8 post-fabrication. Cell spreading and interaction with the extracellular matrix was also markedly increased in high-fibrin matrices. Such composite microbeads containing viable entrapped cells have potential for minimally invasive delivery of cells for a variety of tissue repair applications.

  9. Weight reduction is associated with increased plasma fibrin clot lysis.

    Science.gov (United States)

    Brzezińska-Kolarz, Beata; Kolarz, Marek; Wałach, Angelika; Undas, Anetta

    2014-11-01

    Obesity is associated with an increased risk of vascular thrombotic events. We sought to investigate how obesity and weight loss affect plasma fibrin clot properties. A total of 29 obese patients were studied before and after 3-month low-fat diet. Plasma fibrin clot parameters, including fibrin clot permeation coefficient (Ks), the lag phase of the turbidity curve, clot lysis time (t 50%), maximum rate of increase in D-dimer levels, and maximum D-dimer concentrations, were determined. Low-fat diet resulted in the reduction of body weight (P < .0001), body mass index (P < .0001), fat mass (P < .0001), total cholesterol (P < .0001), low-density lipoprotein cholesterol (P = .0005), triglycerides (P = .008), and plasminogen activator inhibitor 1 (P = .02), but not in fibrinogen or C-reactive protein. The only change in fibrin clot variables was shorter t 50% (P = .02). Baseline t 50%, but not posttreatment, correlated with waist circumference (r = .44, p = .02). This study demonstrates that weight loss in obese people can increase the efficiency of fibrin clot lysis.

  10. 21 CFR 864.7320 - Fibrinogen/fibrin degradation products assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Fibrinogen/fibrin degradation products assay. 864....7320 Fibrinogen/fibrin degradation products assay. (a) Identification. A fibrinogen/fibrin degradation products assay is a device used to detect and measure fibrinogen degradation products and...

  11. A CCR2 macrophage endocytic pathway mediates extravascular fibrin clearance in vivo

    DEFF Research Database (Denmark)

    Motley, Michael P; Madsen, Daniel H; Jürgensen, Henrik J;

    2016-01-01

    Extravascular fibrin deposition accompanies many human diseases and causes chronic inflammation and organ damage, unless removed in a timely manner. Here, we used intravital microscopy to investigate how fibrin is removed from extravascular space. Fibrin placed into the dermis of mice underwent c...

  12. First results from the GlueX experiment

    Science.gov (United States)

    Al Ghoul, H.; Anassontzis, E. G.; Barbosa, F.; Barnes, A.; Beattie, T. D.; Bennett, D. W.; Berdnikov, V. V.; Black, T.; Boeglin, W.; Brooks, W. K.; Cannon, B.; Chernyshov, O.; Chudakov, E.; Crede, V.; Dalton, M. M.; Deur, A.; Dobbs, S.; Dolgolenko, A.; Dugger, M.; Egiyan, H.; Eugenio, P.; Foda, A. M.; Frye, J.; Furletov, S.; Gan, L.; Gasparian, A.; Gerasimov, A.; Gevorgyan, N.; Goryachev, V. S.; Guegan, B.; Guo, L.; Hakobyan, H.; Hakobyan, H.; Hardin, J.; Huber, G. M.; Ireland, D.; Ito, M. M.; Jarvis, N. S.; Jones, R. T.; Kakoyan, V.; Kamel, M.; Klein, F. J.; Kourkoumeli, C.; Kuleshov, S.; Lara, M.; Larin, I.; Lawrence, D.; Leckey, J.; Levine, W. I.; Livingston, K.; Lolos, G. J.; Mack, D.; Mattione, P. T.; Matveev, V.; McCaughan, M.; McGinley, W.; McIntyre, J.; Mendez, R.; Meyer, C. A.; Miskimen, R.; Mitchell, R. E.; Mokaya, F.; Moriya, K.; Nigmatkulov, G.; Ochoa, N.; Ostrovidov, A. I.; Papandreou, Z.; Pedroni, R.; Pennington, M.; Pentchev, L.; Ponosov, A.; Pooser, E.; Pratt, B.; Qiang, Y.; Reinhold, J.; Ritchie, B. G.; Robison, L.; Romanov, D.; Salgado, C.; Schumacher, R. A.; Semenov, A. Yu.; Semenova, I. A.; Senderovich, I.; Seth, K. K.; Shepherd, M. R.; Smith, E. S.; Sober, D. I.; Somov, A.; Somov, S.; Soto, O.; Sparks, N.; Staib, M. J.; Stevens, J. R.; Subedi, A.; Tarasov, V.; Taylor, S.; Tolstukhin, I.; Tomaradze, A.; Toro, A.; Tsaris, A.; Vasileiadis, G.; Vega, I.; Voulgaris, G.; Walford, N. K.; Whitlatch, T.; Williams, M.; Wolin, E.; Xiao, T.; Zarling, J.; Zihlmann, B.

    2016-05-01

    The GlueX experiment at Jefferson Lab ran with its first commissioning beam in late 2014 and the spring of 2015. Data were collected on both plastic and liquid hydrogen targets, and much of the detector has been commissioned. All of the detector systems are now performing at or near design specifications and events are being fully reconstructed, including exclusive production of π0, η and ω mesons. Linearly-polarized photons were successfully produced through coherent bremsstrahlung and polarization transfer to the ρ has been observed.

  13. First Results from The GlueX Experiment

    Energy Technology Data Exchange (ETDEWEB)

    Meyer, Curtis [Carnegie Mellon Univ., Pittsburgh, PA (United States)

    2016-05-01

    The GlueX experiment at Jefferson Lab ran with its first commissioning beam in late 2014 and the spring of 2015. Data were collected on both plastic and liquid hydrogen targets, and much of the detector has been commissioned. All of the detector systems are now performing at or near design specifications and events are being fully reconstructed, including exclusive production of pi^0, eta and omega mesons. Linearly-polarized photons were successfully produced through coherent bremsstrahlung and polarization transfer to the ρ has been observed.

  14. First Results from The GlueX Experiment

    CERN Document Server

    Ghoul, H Al; Barbosa, F; Barnes, A; Beattie, T D; Bennett, D W; Berdnikov, V V; Black, T; Boeglin, W; Brooks, W K; Cannon, B; Chernyshov, O; Chudakov, E; Crede, V; Dalton, M M; Deur, A; Dobbs, S; Dolgolenko, A; Dugger, M; Egiyan, H; Eugenio, P; Foda, A M; Frye, J; Furletov, S; Gan, L; Gasparian, A; Gerasimov, A; Gevorgyan, N; Goryachev, V S; Guegan, B; Guo, L; Hakobyan, H; Hakobyan2, H; Hardin, J; Huber, G M; Ireland, D; Ito, M M; Jarvis, N S; Jones, R T; Kakoyan, V; Kamel, M; Klein, F J; Kourkoumeli, C; Kuleshov, S; Lara, M; Larin, I; Lawrence, D; Leckey, J; Levine, W I; Livingston, K; Lolos, G J; Mack, D; Mattione, P T; Matveev, V; McCaughan, M; McGinley, W; McIntyre, J; Mendez, R; Meyer, C A; Miskimen, R; Mitchell, R E; Mokaya, F; Moriya, K; Nigmatkulov, G; Ochoa, N; Ostrovidov, A I; Papandreou, Z; Pedroni, R; Pennington, M; Pentchev, L; Ponosov, A; Pooser, E; Pratt, B; Qiang, Y; Reinhold, J; Ritchie, B G; Robison, L; Romanov, D; Salgado, C; Schumacher, R A; Semenov, A Yu; Semenova, I A; Senderovich, I; Seth, K K; Shepherd, M R; Smith, E S; Sober, D I; Somov, A; Somov, S; Soto, O; Sparks, N; Staib, M J; Stevens, J R; Subedi, A; Tarasov, V; Taylor, S; Tolstukhin, I; Tomaradze, A; Toro, A; Tsaris, A; Vasileiadis, G; Vega, I; Voulgaris, G; Walford, N K; Whitlatch, T; Williams, M; Wolin, E; Xiao, T; Zarling, J; Zihlmann, B

    2015-01-01

    The GlueX experiment at Jefferson Lab ran with its first commissioning beam in late 2014 and the spring of 2015. Data were collected on both plastic and liquid hydrogen targets, and much of the detector has been commissioned. All of the detector systems are now performing at or near design specifications and events are being fully reconstructed, including exclusive production of $\\pi^{0}$, $\\eta$ and $\\omega$ mesons. Linearly-polarized photons were successfully produced through coherent bremsstrahlung and polarization transfer to the $\\rho$ has been observed.

  15. The GlueX Central Drift Chamber: Design and Performance

    CERN Document Server

    Van Haarlem, Y; Barbosa, F; Dey, B; Lawrence, D; Razmyslovich, V; Smith, E; Visser, G; Whitlatch, T; Wilkin, G; Zihlmann, B

    2010-01-01

    Tests and studies concerning the design and performance of the GlueX Central Drift Chamber (CDC) are presented. A full-scale prototype was built to test and steer the mechanical and electronic design. Small scale prototypes were constructed to test for sagging and to do timing and resolution studies of the detector. These studies were used to choose the gas mixture and to program a Monte Carlo simulation that can predict the detector response in an external magnetic field. Particle identification and charge division possibilities were also investigated.

  16. Egg Drop Competition Involving Only Toothpicks and Glue

    Science.gov (United States)

    Houpt, Stephen

    2004-04-01

    The Winston Science Egg Drop Competition is held annually in November, one of a large group of science competitions organized by Lehman Marks of the Winston School in Dallas, TX. The rules for the competition state that the egg package may be constructed of toothpicks and glue only, with a mass limit of 50 g, not including the egg. Once the egg is placed inside the package, it is dropped from a height of 8 m. The winning entry is the one that makes impact in the least amount of time while still protecting the egg. Timing is electronic.

  17. GLUE!: An Architecture for the Integration of External Tools in Virtual Learning Environments

    Science.gov (United States)

    Alario-Hoyos, Carlos; Bote-Lorenzo, Miguel L.; Gomez-Sanchez, Eduardo; Asensio-Perez, Juan I.; Vega-Gorgojo, Guillermo; Ruiz-Calleja, Adolfo

    2013-01-01

    The integration of external tools in Virtual Learning Environments (VLEs) aims at enriching the learning activities that educational practitioners may design and enact. This paper presents GLUE!, an architecture that enables the lightweight integration of multiple existing external tools in multiple existing VLEs. GLUE! fosters this integration by…

  18. Autologous serum improves bone formation in a primary stable silica-embedded nanohydroxyapatite bone substitute in combination with mesenchymal stem cells and rhBMP-2 in the sheep model

    Directory of Open Access Journals (Sweden)

    Boos AM

    2014-11-01

    Full Text Available Anja M Boos,1,* Annika Weigand,1,* Gloria Deschler,1 Thomas Gerber,2 Andreas Arkudas,1 Ulrich Kneser,1 Raymund E Horch,1 Justus P Beier11Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander-University of Erlangen-Nürnberg FAU, Erlangen, 2Institute of Physics, University of Rostock, Rostock, Germany *These authors contributed equally to this work Abstract: New therapeutic strategies are required for critical size bone defects, because the gold standard of transplanting autologous bone from an unharmed area of the body often leads to several severe side effects and disadvantages for the patient. For years, tissue engineering approaches have been seeking a stable, axially vascularized transplantable bone replacement suitable for transplantation into the recipient bed with pre-existing insufficient conditions. For this reason, the arteriovenous loop model was developed and various bone substitutes have been vascularized. However, it has not been possible thus far to engineer a primary stable and axially vascularized transplantable bone substitute. For that purpose, a primary stable silica-embedded nanohydroxyapatite (HA bone substitute in combination with blood, bone marrow, expanded, or directly retransplanted mesenchymal stem cells, recombinant human bone morphogenetic protein 2 (rhBMP-2, and different carrier materials (fibrin, cell culture medium, autologous serum was tested subcutaneously for 4 or 12 weeks in the sheep model. Autologous serum lead to an early matrix change during degradation of the bone substitute and formation of new bone tissue. The best results were achieved in the group combining mesenchymal stem cells expanded with 60 µg/mL rhBMP-2 in autologous serum. Better ingrowth of fibrovascular tissue could be detected in the autologous serum group compared with the control (fibrin. Osteoclastic activity indicating an active bone remodeling process was observed after 4 weeks, particularly

  19. Spray- and laser-assisted biomaterial processing for fast and efficient autologous cell-plus-matrix tissue engineering.

    Science.gov (United States)

    Klopsch, Christian; Gäbel, Ralf; Kaminski, Alexander; Mark, Peter; Wang, Weiwei; Toelk, Anita; Delyagina, Evgenya; Kleiner, Gabriela; Koch, Lothar; Chichkov, Boris; Mela, Petra; Jockenhoevel, Stefan; Ma, Nan; Steinhoff, Gustav

    2015-12-01

    At present, intensive investigation aims at the creation of optimal valvular prostheses. We introduced and tested the applicability and functionality of two advanced cell-plus-matrix seeding technologies, spray-assisted bioprocessing (SaBP) and laser-assisted bioprocessing (LaBP), for autologous tissue engineering (TE) of bioresorbable artificial grafts. For SaBP, human mesenchymal stem cells (HMSCs), umbilical cord vein endothelial cells (HUVECs) and fibrin were simultaneously spray-administered on poly(ε-caprolactone) (PCL) substrates. For LaBP, HUVECs and HMSCs were separately laser-printed in stripes, followed by fibrin sealing. Three-leaflet valves were manufactured following TE of electrospun PCL tissue equivalents. Grafts were monitored in vitro under static and dynamic conditions in bioreactors. SaBP and LaBP resulted in TE of grafts with homogeneous cell distribution and accurate cell pattern, respectively. The engineered valves demonstrated immediate sufficient performance, complete cell coating, proliferation, engraftment, HUVEC-mediated invasion, HMSC differentiation and extracellular matrix deposition. SaBP revealed higher efficiency, with at least 12-fold shorter processing time than the applied LaBP set-up. LaBP realized coating with higher cell density and minimal cell-scaffold distance. Fibrin and PCL stability remain issues for improvement. The introduced TE technologies resulted in complete valvular cell-plus-matrix coating, excellent engraftment and HMSCs differentiation. SaBP might have potential for intraoperative table-side TE considering the procedural duration and ease of implementation. LaBP might accelerate engraftment with precise patterns.

  20. Discrimination between Fibrin and Fibrinogen by a Monoclonal Antibody against a Synthetic Peptide

    Science.gov (United States)

    Scheefers-Borchel, Ursula; Muller-Berghaus, Gert; Fuhge, Peter; Eberle, Reinhard; Heimburger, Nobert

    1985-10-01

    Circulating soluble fibrin, observed in the blood of patients with ongoing intravascular coagulation, is generated from the plasma protein fibrinogen by the limited proteolytic action of thrombin. We report the production of a monoclonal antibody that discriminates between fibrin and fibrinogen in blood. The synthetic hexapeptide Gly-Pro-Arg-Val-Val-Glu, representing the amino terminus of the α chain of human fibrin, was used as immunogen. This hexapeptide is located within the Aα chain of fibrinogen but becomes the amino terminus of the fibrin α chain, after fibrinopeptide A is removed by the action of thrombin, and thus becomes accessible for antibody binding. The monoclonal antibody we have prepared can discriminate between fibrin and fibrinogen and thus can be used in assay systems to quantitate soluble fibrin or, potentially, to image fibrin-rich thrombi.

  1. Autologous Fat Grafting for Whole Breast Reconstruction

    Directory of Open Access Journals (Sweden)

    Benjamin H. L. Howes, MBBS

    2014-03-01

    Full Text Available Summary: This is the first reported case of a patient who had a single-stage large-volume breast reconstruction with autologous fat grafting, following rotation flap approach (RoFA mastectomy. The purpose of this case study was to evaluate the viability of reconstruction of the breast by autologous fat grafting alone, in the context of RoFA mastectomy. The hypothesis was that there would be minimal interval loss of autologous fat on the whole breast reconstruction side. Right RoFA mastectomy was used for resection of an invasive primary breast cancer and resulted in the right breast skin envelope. Eleven months later, the patient underwent grafting of 400 ml of autologous fat into the skin envelope and underlying pectoralis major muscle. Outcome was assessed by using a validated 3D laser scan technique for quantitative breast volume measurement. Other outcome measures included the BREAST-Q questionnaire and 2D clinical photography. At 12-month follow-up, the patient was observed to have maintenance of volume of the reconstructed breast. Her BREAST-Q scores were markedly improved compared with before fat grafting, and there was observable improvement in shape, contour, and symmetry on 2D clinical photography. The 2 new techniques, RoFA mastectomy and large-volume single-stage autologous fat grafting, were used in combination to achieve a satisfactory postmastectomy breast reconstruction. Novel tools for measurement of outcome were the 3D whole-body laser scanner and BREAST-Q questionnaire. This case demonstrates the potential for the use of fat grafting for reconstruction. Outcomes in a larger patient populations are needed to confirm these findings.

  2. Calix[4]arene methylenebisphosphonic acids as inhibitors of fibrin polymerization.

    NARCIS (Netherlands)

    Lugovskoy, E.V.; Gritsenko, P.; Koshel, T.A.; Koliesnik, I.O.; Cherenok, S.O.; Kalchenko, O.I.; Kalchenko, V.I.; Komisarenko, S.V.

    2011-01-01

    Calix[4]arenes bearing two or four methylenebisphosphonic acid groups at the macrocyclic upper rim have been studied with respect to their effects on fibrin polymerization. The most potent inhibitor proved to be calix[4]arene tetrakis-methylene-bis-phosphonic acid (C-192), in which case the maximum

  3. Concentration independent modulation of local micromechanics in a fibrin gel.

    Directory of Open Access Journals (Sweden)

    Maxwell A Kotlarchyk

    Full Text Available Methods for tuning extracellular matrix (ECM mechanics in 3D cell culture that rely on increasing the concentration of either protein or cross-linking molecules fail to control important parameters such as pore size, ligand density, and molecular diffusivity. Alternatively, ECM stiffness can be modulated independently from protein concentration by mechanically loading the ECM. We have developed a novel device for generating stiffness gradients in naturally derived ECMs, where stiffness is tuned by inducing strain, while local mechanical properties are directly determined by laser tweezers based active microrheology (AMR. Hydrogel substrates polymerized within 35 mm diameter Petri dishes are strained non-uniformly by the precise rotation of an embedded cylindrical post, and exhibit a position-dependent stiffness with little to no modulation of local mesh geometry. Here we present the device in the context of fibrin hydrogels. First AMR is used to directly measure local micromechanics in unstrained hydrogels of increasing fibrin concentration. Changes in stiffness are then mapped within our device, where fibrin concentration is held constant. Fluorescence confocal imaging and orbital particle tracking are used to quantify structural changes in fibrin on the micro and nano levels respectively. The micromechanical strain stiffening measured by microrheology is not accompanied by ECM microstructural changes under our applied loads, as measured by confocal microscopy. However, super-resolution orbital tracking reveals nanostructural straightening, lengthening, and reduced movement of fibrin fibers. Furthermore, we show that aortic smooth muscle cells cultured within our device are morphologically sensitive to the induced mechanical gradient. Our results demonstrate a powerful cell culture tool that can be used in the study of mechanical effects on cellular physiology in naturally derived 3D ECM tissues.

  4. Retreatment of endodontically failed tooth with wide-open apex using platelet rich fibrin membrane as matrix and an apical plug of Biodentine™

    Directory of Open Access Journals (Sweden)

    Ajinkya Mansing Pawar

    2015-01-01

    Full Text Available The primary reason for an endodontic failure is the persistence or regrowth of bacteria within the root canal system, and such cases require retreatment. The tooth root development and closure of its apex occurs till 3 years after the eruption. Traumatic injuries during this development period result in endodontic complications. While dealing with a tooth, with an open apex the prime objective was eliminating bacteria from the root canal system with minimum irritation to the periapical tissues and induction of apical closure to produce favorable conditions and to confine the root canal filling within the canal space. Traditionally as supported by literature multiple dressings of calcium hydroxide medicament were advocated to induce apical barrier formation followed by an evolution of the apical artificial barrier technique where the mineral trioxide aggregate (MTA was used. Recently introduced Biodentine™ is similar to MTA with its basic composition, which can be used as its substitute. The main difficulty associated while treating teeth with wide-open apices are preventing the overfilling of the restorative materials that serve as an artificial barrier. Use of a matrix overcomes this challenge. Platelet-rich fibrin (PRF a matrix of autologous fibrin, embedded with a large quantity of platelet and leukocyte cytokines during centrifugation can be successfully used as an apical membrane. The present case, reports a novel procedure of apexification of endodontically failed central incisor with open apex using PRF as apical membrane and Biodentine™.

  5. Magnesium-enhanced enzymatically mineralized platelet-rich fibrin for bone regeneration applications.

    Science.gov (United States)

    Gassling, Volker; Douglas, Timothy E L; Purcz, Nicolai; Schaubroeck, David; Balcaen, Lieve; Bliznuk, Vitaliy; Declercq, Heidi A; Vanhaecke, Frank; Dubruel, Peter

    2013-10-01

    Membranes of the autologous blood-derived biomaterial platelet-rich fibrin (PRF) were mineralized enzymatically with calcium phosphate (CaP) by the incorporation of alkaline phosphatase (ALP) followed by incubation for 3 days in solutions of either 0.1 M calcium glycerophosphate (CaGP) or a combination of CaGP and magnesium glycerophosphate (CaGP:MgGP; both 0.05 M), resulting in the formation of two different PRF-mineral composites. Fourier transform infrared spectroscopy, transmission electron microscopy and selected area electron diffraction examinations showed that the CaP formed was amorphous. Inductively coupled plasma optical emission spectroscopy analysis revealed similar amounts of Ca and P in both composite types, while a smaller amount of Mg (Ca:Mg molar ratio = 10) was detected in the composites formed in the CaGP:MgGP solution, which was supported by the results of energy-dispersive x-ray spectroscopy-based elemental mapping. Scanning electron microscopy (SEM) imaging showed that the mineral deposits in PRF incubated in the CaGP:MgGP solution were markedly smaller. The mass percentage attributable to the mineral phase was similar in both composite types. MTT and WST tests with SAOS-2 cells revealed that incubation in the CaGP:MgGP solution had no negative effect on cytocompatibility and cell proliferation compared to the CaGP solution. Cells on all samples displayed a well-spread morphology as revealed by SEM imaging. In conclusion, the incorporation of Mg reduces mineral deposit dimensions and promotes cell proliferation.

  6. Event based uncertainty assessment in urban drainage modelling, applying the GLUE methodology

    DEFF Research Database (Denmark)

    Thorndahl, Søren; Beven, K.J.; Jensen, Jacob Birk

    2008-01-01

    In the present paper an uncertainty analysis on an application of the commercial urban drainage model MOUSE is conducted. Applying the Generalized Likelihood Uncertainty Estimation (GLUE) methodology the model is conditioned on observation time series from two flow gauges as well as the occurrence...... of combined sewer overflow. The GLUE methodology is used to test different conceptual setups in order to determine if one model setup gives a better goodness of fit conditional on the observations than the other. Moreover, different methodological investigations of GLUE are conducted in order to test...

  7. Relative gain monitoring of the GlueX calorimeters

    Science.gov (United States)

    Anassontzis, E. G.; Ioannou, P.; Kourkoumelis, C.; Vasileiadis, G.; Voulgaris, G.; Kappos, E.; Beattie, T.; Krueger, S.; Lolos, G. J.; Papandreou, Z.; Semenov, A. Yu.; Frye, J.; Leckey, J.; Shepherd, M. R.; Bogart, T.; Lawrence, D.; Smith, E. S.

    2014-02-01

    The relative gain of the photodetectors for the GlueX Barrel and Forward calorimeters will be monitored using modular LED driver systems. The BCAL system consists of a global controller that feeds power, bias voltage and trigger signals to 96 local controllers situated at the ends of the 48 BCAL modules, which drive 40 LEDs associated with the 40 light guides at the end of each module. The FCAL system consists also of a global controller, a local controller for each acrylic quadrant covering the face of the FCAL, and ten 4-LED pulser boards per local controller connected in a star configuration along the edges of the acrylic panes. The respective systems are currently being installed on the detectors and their tested performance is presented herein.

  8. Elastic behavior and platelet retraction in low- and high-density fibrin gels.

    Science.gov (United States)

    Wufsus, Adam R; Rana, Kuldeepsinh; Brown, Andrea; Dorgan, John R; Liberatore, Matthew W; Neeves, Keith B

    2015-01-06

    Fibrin is a biopolymer that gives thrombi the mechanical strength to withstand the forces imparted on them by blood flow. Importantly, fibrin is highly extensible, but strain hardens at low deformation rates. The density of fibrin in clots, especially arterial clots, is higher than that in gels made at plasma concentrations of fibrinogen (3-10 mg/mL), where most rheology studies have been conducted. Our objective in this study was to measure and characterize the elastic regimes of low (3-10 mg/mL) and high (30-100 mg/mL) density fibrin gels using shear and extensional rheology. Confocal microscopy of the gels shows that fiber density increases with fibrinogen concentration. At low strains, fibrin gels act as thermal networks independent of fibrinogen concentration. Within the low-strain regime, one can predict the mesh size of fibrin gels by the elastic modulus using semiflexible polymer theory. Significantly, this provides a link between gel mechanics and interstitial fluid flow. At moderate strains, we find that low-density fibrin gels act as nonaffine mechanical networks and transition to affine mechanical networks with increasing strains within the moderate regime, whereas high-density fibrin gels only act as affine mechanical networks. At high strains, the backbone of individual fibrin fibers stretches for all fibrin gels. Platelets can retract low-density gels by >80% of their initial volumes, but retraction is attenuated in high-density fibrin gels and with decreasing platelet density. Taken together, these results show that the nature of fibrin deformation is a strong function of fibrin fiber density, which has ramifications for the growth, embolization, and lysis of thrombi.

  9. Autologous antibodies that bind neuroblastoma cells.

    Science.gov (United States)

    Sun, Yujing; Sholler, Giselle S; Shukla, Girja S; Pero, Stephanie C; Carman, Chelsea L; Zhao, Ping; Krag, David N

    2015-11-01

    Antibody therapy of neuroblastoma is promising and our goal is to derive antibodies from patients with neuroblastoma for developing new therapeutic antibodies. The feasibility of using residual bone marrow obtained for clinical indications as a source of tumor cells and a source of antibodies was assessed. From marrow samples, neuroblastoma cells were recovered, grown in cell culture and also implanted into mice to create xenografts. Mononuclear cells from the marrow were used as a source to generate phage display antibody libraries and also hybridomas. Growth of neuroblastoma patient cells was possible both in vitro and as xenografts. Antibodies from the phage libraries and from the monoclonal hybridomas bound autologous neuroblastoma cells with some selectivity. It appears feasible to recover neuroblastoma cells from residual marrow specimens and to generate human antibodies that bind autologous neuroblastoma cells. Expansion of this approach is underway to collect more specimens, optimize methods to generate antibodies, and to evaluate the bioactivity of neuroblastoma-binding antibodies.

  10. Cryptococcal meningitis post autologous stem cell transplantation.

    Science.gov (United States)

    Chaaban, S; Wheat, L J; Assi, M

    2014-06-01

    Disseminated Cryptococcus disease occurs in patients with defective T-cell immunity. Cryptococcal meningitis following autologous stem cell transplant (SCT) has been described previously in only 1 patient, 4 months post SCT and while off antifungal prophylaxis. We present a unique case of Cryptococcus meningitis pre-engraftment after autologous SCT, while the patient was receiving fluconazole prophylaxis. A 41-year-old man with non-Hodgkin's lymphoma underwent autologous SCT. Post-transplant prophylaxis consisted of fluconazole 400 mg daily, levofloxacin 500 mg daily, and acyclovir 800 mg twice daily. On day 9 post transplant, he developed fever and headache. Peripheral white blood cell count (WBC) was 700/μL. Magnetic resonance imaging of the brain showed lesions consistent with meningoencephalitis. Cerebrospinal fluid (CSF) analysis revealed a WBC of 39 with 77% lymphocytes, protein 63, glucose 38, CSF pressure 20.5 cmH2 O, and a positive cryptococcal antigen. CSF culture confirmed Cryptococcus neoformans. The patient was treated with liposomal amphotericin B 5 mg/kg intravenously daily, and flucytosine 37.5 mg/kg orally every 6 h. He was switched to fluconazole 400 mg daily after 3 weeks of amphotericin therapy, with sterilization of the CSF with negative CSFCryptococcus antigen and negative CSF culture. Review of the literature revealed 9 cases of cryptococcal disease in recipients of SCT. Median time of onset was 64 days post transplant. Only 3 meningitis cases were described; 2 of them after allogeneic SCT. Fungal prophylaxis with fluconazole post autologous SCT is recommended at least through engraftment, and for up to 100 days in high-risk patients. A high index of suspicion is needed to diagnose and treat opportunistic infections, especially in the face of immunosuppression and despite adequate prophylaxis. Infection is usually fatal without treatment, thus prompt diagnosis and therapy might be life saving.

  11. Recovery of autologous erythrocytes in transfused patients.

    Science.gov (United States)

    Wallas, C H; Tanley, P C; Gorrell, L P

    1980-01-01

    A microcapillary method utilizing phthalate esters or an ultracentrifuge method are both capable of separating autologous from homologous erythrocytes in polytransfused patients. The microcapillary technique which is readily adaptable to blood bank laboratories provides a previously unavailable method for defining blood group antigen typings in transfused patients. Such typings are of vital importance in the laboratory evaluation of transfused patients with multiple or weak blood group antibodies.

  12. The mechanics of fibrin networks and their alterations by platelets

    Science.gov (United States)

    Jawerth, Louise Marie

    Fibrin is a biopolymer that assembles into a network during blood coagulation to become the structural scaffold of a blood clot. The precise mechanics of this network are crucial for a blood clot to properly stem the flow of blood at the site of vascular injury while still remaining pliable enough to avoid dislocation. A hallmark of fibrin's mechanical response is strain-stiffening: at small strains, its response is low and linear; while at high strains, its stiffness increases non-linearly with increasing strain. The physical origins of strain-stiffening have been studied for other biopolymer systems but have remained elusive for biopolymer networks composed of stiff filaments, such as fibrin. To understand the origins of this intriguing behavior, we directly observe and quantify the motion of all of the fibers in the fibrin networks as they undergo shear in 3D using confocal microscopy. We show that the strain-stiffening response of a clot is a result of the full network deformation rather than an intrinsic strain-stiffening response of the individual fibers. We observe a distinct transition from a linear, low-strain regime, where all fibers avoid any internal stretching, to a non-linear, high-strain regime, where an increasing number of fibers become stretched. This transition is characterized by a high degree of non-affine motion. Moreover, we are able to precisely calculate the non-linear stress-strain response of the network by using the strains on each fiber measured directly with confocal microscopy and by assuming the fibers behave like linearly elastic beams. This result confirms that it is the network deformation that causes the strain-stiffening behavior of fibrin clots. These data are consistent with predictions for low-connectivity networks with soft, bending, or floppy modes. Moreover, we show that the addition of small contractile cells, platelets, increases the low-strain stiffness of the network while the high-strain stiffness is independent of

  13. Facial wrinkles correction through autologous fat microinjection.

    Directory of Open Access Journals (Sweden)

    Heriberto Cháves Sánchez

    2008-12-01

    Full Text Available Background: autologous fat microinjection is a technique which allows the correction of different dispositions that appear in the face in a very fast, effective and simple way compared to other procedures implying more pain, incisions, and elevated doses of anesthesia. Objective: to show the effectiveness of the autologous fat microinjection in the correction of facial wrinkles. Methods: a series study was carried out from May 2005 to May 2006 at the University Hospital “Dr. Gustavo Aldereguía Lima” in Cienfuegos city, Cuba. 60 patients of both sexes constituted this series study. They had facial wrinkles and this procedure was performed on them. Age, sex, patient’s race, localization and the type of wrinkle as well as the satisfaction level of the patient with the surgical procedure were analyzed. Results: Female sex was predominant, as well as white race and the ages from 45-50. A good aesthetic result was obtained. The satisfaction level of the patients was more elevated in short and medium terms. Conclusions: the level of satisfaction reached in the studied series reassure the advantages of the autologous fat microinjection technique so that, it is recommended for the elimination of facial wrinkles.

  14. 9 CFR 95.9 - Glue stock; requirements for unrestricted entry.

    Science.gov (United States)

    2010-01-01

    ... render each piece of the hardness of a sun-dried hide. (c) Glue stock taken from cattle, sheep, goats, or... bearing the seal of the proper department of said national government and signed by an official...

  15. Generalized likelihood uncertainty estimation (GLUE) using adaptive Markov chain Monte Carlo sampling

    DEFF Research Database (Denmark)

    Blasone, Roberta-Serena; Vrugt, Jasper A.; Madsen, Henrik

    2008-01-01

    estimate of the associated uncertainty. This uncertainty arises from incomplete process representation, uncertainty in initial conditions, input, output and parameter error. The generalized likelihood uncertainty estimation (GLUE) framework was one of the first attempts to represent prediction uncertainty...

  16. Using autologous platelet-rich plasma for the treatment of complex fistulas

    Directory of Open Access Journals (Sweden)

    Almudena Moreno-Serrano

    Full Text Available Objective: This study aims to demonstrate the effectiveness and safety of autologous fibrin gel rich in platelet growth factors for the treatment of complex perianal fistulas. Material and methods: Prospective epidemiological study. Patients with complex perianal fistula or perianal fistula mere alteration of continence are included. identification of both holes and the journey, curettage of it and instillation of Vivostat PRF® in the way it is done to observe excess material by OFE. The variables analyzed were: age, sex, use of prior Seton clinic prevalent type of fistula, postoperative complications, fistula closure and impaired quality of life using the SF-36 test (v2. Results: From January 2011 to May 2013 have involved 23 patients, 12 men and 11 women, with an average age of 49 years and a minimum follow-up of 12 months. Two dropped out. 17 patients had low transsphincteric fistulas, 2 and 2 high transsphincteric intersphincteric with impaired continence. The most common symptom is the discharge. Twelve patients had a loose seton (62%, of which nine cured. Of all the patients we have operated the success rate is 62%. No patient developed incontinence after treatment. Only two reported a worse quality of life after surgery. Conclusion: This study demonstrates that there is a clear benefit to the use of Vivostat PRF® as a treatment for complex perianal fistulas. It is a highly reproducible technique with acceptable results and does not produce impairment of continence.

  17. Calibration of cathode strip gains in multiwire drift chambers of the GlueX experiment

    Energy Technology Data Exchange (ETDEWEB)

    Berdnikov, V. V.; Somov, S. V.; Pentchev, L.; Somov, A.

    2016-07-01

    A technique for calibrating cathode strip gains in multiwire drift chambers of the GlueX experiment is described. The accuracy of the technique is estimated based on Monte Carlo generated data with known gain coefficients in the strip signal channels. One of the four detector sections has been calibrated using cosmic rays. Results of drift chamber calibration on the accelerator beam upon inclusion in the GlueX experimental setup are presented.

  18. Double Coaxial Microcatheter Technique for Glue Embolization of Renal Arteriovenous Malformations

    Energy Technology Data Exchange (ETDEWEB)

    Uchikawa, Yoko, E-mail: jauchikawa@gmail.com [University of Tsukuba Hospital, Department of Radiology (Japan); Mori, Kensaku, E-mail: moriken@md.tsukuba.ac.jp [University of Tsukuba, Department of Radiology, Faculty of Medicine (Japan); Shiigai, Masanari, E-mail: m-41gai@yahoo.co.jp [Tsukuba Medical Center Hospital, Department of Radiology (Japan); Konishi, Takahiro, E-mail: soratobukangaruu@gmail.com [University of Tsukuba Hospital, Department of Radiology (Japan); Hoshiai, Sodai, E-mail: hoshiai@sb4.so-net.ne.jp [Ibaraki Prefectural Central Hospital, Department of Radiology (Japan); Ishigro, Toshitaka, E-mail: suzutokei@gmail.com; Hiyama, Takashi, E-mail: med-tak@hotmail.com [University of Tsukuba Hospital, Department of Radiology (Japan); Nakai, Yasunobu, E-mail: nakaiya@tmch.or.jp [Tsukuba Medical Center Hospital, Department of Neurosurgery (Japan); Minami, Manabu, E-mail: mminami@md.tsukuba.ac.jp [University of Tsukuba, Department of Radiology, Faculty of Medicine (Japan)

    2015-10-15

    PurposeTo demonstrate the technical benefit of the double coaxial microcatheter technique for embolization of renal arteriovenous malformations (AVMs) with n-butyl cyanoacrylate and iodized oil (glue).Materials and MethodsSix consecutive patients (1 man and 5 women; mean age 61 years; range 44–77 years) with renal AVMs were included. Five patients had hematuria, and one had a risk of heart failure due to a large intrarenal arteriovenous shunt. All patients underwent transarterial embolization using glue and the double coaxial microcatheter technique with outer 2.6F and inner 1.9F microcatheters. After glue injection, the inner microcatheter was retracted, while the outer microcatheter was retained. We assessed the complications and clinical outcomes of this technique.ResultsTechnical success was achieved in all patients. In 9 sessions, 34 feeding arteries were embolized with glue using the double coaxial microcatheter technique, 1 was embolized with glue using a single microcatheter, and 2 were embolized with coils. The double coaxial microcatheter technique was useful for selecting small tortuous feeding arteries, preventing glue reflux to the proximal arteries, and approaching multiple feeding arteries without complete retraction of the microcatheters. As a minor complication, glue migrated into the venous system in four patients without any sequelae. In all patients, favorable clinical outcomes, including hematuria cessation in five patients and improvement of the large intrarenal arteriovenous shunt in one patient, were obtained without deterioration of renal function.ConclusionGlue embolization with the double coaxial microcatheter technique was useful for treating renal AVMs with multiple tortuous feeding arteries.

  19. Glue for Sealing Internal Pancreatic Fistula in a Patient with Liver Cirrhosis: A Useful Technique

    Directory of Open Access Journals (Sweden)

    Mahesh Kumar Goenka

    2012-05-01

    Full Text Available Context Pancreatic fistulae are uncommon and usually follow acute or chronic pancreatitis. While most of these are treatedconservatively, some require surgery. Recently endoscopic therapy has emerged as an effective alternative treatment modality. Case report We present a patient with internal pancreatic fistula due to alcohol related chronic pancreatitis. Endotherapy using glue resulted in resolution of the fistula. Conclusion The use of endoscopic glue injection may be a safe and effective method for the successful therapy of internal pancreatic fistula.

  20. Spider web glue: two proteins expressed from opposite strands of the same DNA sequence.

    Science.gov (United States)

    Choresh, Omer; Bayarmagnai, Battuya; Lewis, Randolph V

    2009-10-12

    The various silks that make up the web of the orb web spiders have been studied extensively. However, success in prey capture depends as much on the web glue as on the fibers. Spider silk glue, which is considered one of the strongest and most effective biological glues, is an aqueous solution secreted from the orb weaving spider's aggregate glands and coats the spiral prey capturing threads of their webs. Studies identified the major component of the glue as microscopic nodules made of a glycoprotein. This study describes two newly discovered proteins that form the glue-glycoprotein of the golden orb weaving spider Nephila clavipes . Our results demonstrate that both proteins contain unique 110 amino acid repetitive domains that are encoded by opposite strands of the same DNA sequence. Thus, the genome of the spider encodes two distinct yet functionally related genes by using both strands of an identical DNA sequence. Moreover, the closest match for the nonrepetitive region of one of the proteins is chitin binding proteins. The web glue appears to have evolved a substantial level of sophistication matching that of the spider silk fibers.

  1. Evidence from Multiple Species that Spider Silk Glue Component ASG2 is a Spidroin.

    Science.gov (United States)

    Collin, Matthew A; Clarke, Thomas H; Ayoub, Nadia A; Hayashi, Cheryl Y

    2016-02-15

    Spiders in the superfamily Araneoidea produce viscous glue from aggregate silk glands. Aggregate glue coats prey-capture threads and hampers the escape of prey from webs, thereby increasing the foraging success of spiders. cDNAs for Aggregate Spider Glue 1 (ASG1) and 2 (ASG2) have been previously described from the golden orb-weaver, Nephila clavipes, and Western black widow, Latrodectus hesperus. To further investigate aggregate glues, we assembled ASG1 and ASG2 from genomic target capture libraries constructed from three species of cob-web weavers and three species of orb-web weavers, all araneoids. We show that ASG1 is unlikely to be a glue, but rather is part of a widespread arthropod gene family, the peritrophic matrix proteins. For ASG2, we demonstrate its remarkable architectural and sequence similarities to spider silk fibroins, indicating that ASG2 is a member of the spidroin gene family. Thus, spidroins have diversified into glues in addition to task-specific, high performance fibers.

  2. Evidence from Multiple Species that Spider Silk Glue Component ASG2 is a Spidroin

    Science.gov (United States)

    Collin, Matthew A.; Clarke, Thomas H.; Ayoub, Nadia A.; Hayashi, Cheryl Y.

    2016-01-01

    Spiders in the superfamily Araneoidea produce viscous glue from aggregate silk glands. Aggregate glue coats prey-capture threads and hampers the escape of prey from webs, thereby increasing the foraging success of spiders. cDNAs for Aggregate Spider Glue 1 (ASG1) and 2 (ASG2) have been previously described from the golden orb-weaver, Nephila clavipes, and Western black widow, Latrodectus hesperus. To further investigate aggregate glues, we assembled ASG1 and ASG2 from genomic target capture libraries constructed from three species of cob-web weavers and three species of orb-web weavers, all araneoids. We show that ASG1 is unlikely to be a glue, but rather is part of a widespread arthropod gene family, the peritrophic matrix proteins. For ASG2, we demonstrate its remarkable architectural and sequence similarities to spider silk fibroins, indicating that ASG2 is a member of the spidroin gene family. Thus, spidroins have diversified into glues in addition to task-specific, high performance fibers. PMID:26875681

  3. Dynamics of spider glue adhesion: effect of surface energy and contact area

    Science.gov (United States)

    Amarpuri, Gaurav; Chen, Yizhou; Blackledge, Todd; Dhinojwala, Ali

    Spider glue is a unique biological adhesive which is humidity responsive such that the adhesion continues to increase upto 100% relative humidity (RH) for some species. This is unlike synthetic adhesives that significantly drop in adhesion with an increase in humidity. However, most of adhesion data reported in literature have used clean hydrophilic glass substrate, unlike the hydrophobic, and charged insect cuticle surface that adheres to spider glue in nature. Previously, we have reported that the spider glue viscosity changes over five orders of magnitude with humidity. Here, we vary the surface energy and surface charge of the substrate to test the change in Larnioides cornutus spider glue adhesion with humidity. We find that an increase in both surface energy and surface charge density increases the droplet spreading and there exists an optimum droplet contact area where adhesion is maximized. Moreover, spider glue droplets act as reusable adhesive for low energy hydrophobic surface at the optimum humidity. These results explain why certain prey are caught more efficiently by spiders in their habitat. The mechanism by which spider species tune its glue adhesion for local prey capture can inspire new generation smart adhesives.

  4. A Novel Role for Erythropoietin During Fibrin-Induced Wound-Healing Response

    OpenAIRE

    Haroon, Zishan A.; Amin, Khalid; Jiang, XiaoHong; Arcasoy, Murat O.

    2003-01-01

    In this study, we investigated the role of the hematopoietic cytokine erythropoietin (EPO) during wound healing, the physiological response to tissue injury. We used an in vivo wound-healing assay (fibrin Z-chambers) consisting of fibrin-filled chambers implanted subcutaneously in rats. The fibrin inside the chambers is replaced by granulation tissue consisting of new blood vessels, macrophages and fibroblasts as part of the wound-healing response. Local, exogenous recombinant EPO administrat...

  5. Fibrin gel-immobilized primary osteoblasts in calcium phosphate bone cement: in vivo evaluation with regard to application as injectable biological bone substitute.

    Science.gov (United States)

    Kneser, U; Voogd, A; Ohnolz, J; Buettner, O; Stangenberg, L; Zhang, Y H; Stark, G B; Schaefer, D J

    2005-01-01

    Osteogenic injectable bone substitutes may be useful for many applications. We developed a novel injectable bone substitute based on osteoblast-fibrin glue suspension and calcium phosphate bone cement (BC). Human osteoblasts were isolated from trabecular bone samples and cultured under standard conditions. Osteoblasts were suspended in fibrinogen solution (FS). BC was cured with thrombin solution. 8 x 4 mm injectable bone discs were prepared using silicon molds and a custom-made applicator device. Discs containing BC, BC/FS, or BC/FS/osteoblasts were implanted subcutaneously into athymic nude mice. After 3, 9 and 24 weeks, specimens were explanted and subjected to morphologic and biomechanical evaluation. In vitro fibrin gel-embedded osteoblasts displayed a differentiated phenotype as evidenced by alkaline phosphatase, collagen type 1 and von Kossa stains. A proportion of osteoblasts appeared morphologically intact over a 3-day in vitro period following application into the BC. BC/FS and BC/FS/osteoblast discs were sparsely infiltrated with vascularized connective tissue. There was no bone formation in implants from all groups. However, positive von Kossa staining only in BC/FS/osteoblast groups suggests engraftment of at least some of the transplanted cells. Biomechanical evaluation demonstrated initial stability of the composites. Young's modulus and maximal load did not differ significantly in the BC/FS and BC/FS/osteoblast groups. The practicability of osteoblast-containing injectable bone could be demonstrated. The dense microstructure and the suboptimal initial vascularization of the composites may explain the lack of bone formation. Modifications with regard to enhanced osteoblast survival are mandatory for a possible application as injectable osteogenic bone replacement system.

  6. Autologus Platelet Rich Fibrin aided Revascularization of an immature, non-vital permanent tooth with apical periodontitis: A case report.

    Science.gov (United States)

    Jadhav, Ganesh Ranganath; Shah, Dipali; Raghvendra, Srinidhi Surya

    2015-01-01

    Caries or trauma induced non-vital immature permanent tooth with blunderbuss, thin root which are very common among childrens are corrected using regenerative endodontic (revascularization) procedures. In the presented case, a 16-year-old boy reported with chief complaint of pain in maxillary left central incisor (Tooth #21). Tooth #21 showed grade III mobility, draining labial sinus, and short blunderbuss root with diffuse periapical radiolucency. Patient was explained the treatment plan and written informed consent was taken. Platelet rich fibrin (PRF) was prepared according to standard protocol. Autologous PRF was carried to the apical portion of the root canal after inducing revascularization. Access opening was double sealed with MTA and resin modified glass ionomer cement (RMGI). Baseline, 12 month and 18 month follow-up intraoral radiographs were taken. Clinically case was asymptomatic with complete resolution of intraoral sinus. Periapical healing, apical closure, root lengthening and dentinal wall thickening were uneventful. Thus PRF supplementation hastens the predictability and rate of revascularization in non-vital immature permanent teeth.

  7. Surgical Re-entry of an Intentionally Replanted Periodontally Compromised Tooth Treated with Platelet Rich Fibrin (PRF): Hopeless to Hopeful.

    Science.gov (United States)

    Ryana, Haneet Kour; Srinath, Rashmi; Prakash, Shobha

    2016-06-01

    Intentional replantation is generally contraindicated in periodontally compromised teeth however, there are reports suggesting that it can be a successful treatment alternative for periodontally involved hopeless teeth. Currently there is dearth of evidence regarding the success of this therapy, especially evidence for the effectiveness of autologous platelet rich fibrin is lacking. We present a case report of a 23-year-old male patient with periodontally hopeless left maxillary central incisor having bone loss extending beyond root apex. The tooth was gently extracted and replanted utilizing root conditioning and combined regenerative therapy (Xenograft, PRF and Type I Collagen Membrane). Surgical re-entry at nine months revealed bone formation in the apical third of the tooth. At one year, 87% radiographic bone gain was accomplished. The improvement in the clinical and radiographic parameters reinforced by the re-entry surgery findings strongly suggest that intentional replantation may be a cost-effective substitute to implants and tooth supported prosthesis in situations where conventional periodontal therapy would yield compromised outcomes.

  8. Surgical Re-entry of an Intentionally Replanted Periodontally Compromised Tooth Treated with Platelet Rich Fibrin (PRF): Hopeless to Hopeful

    Science.gov (United States)

    Srinath, Rashmi; Prakash, Shobha

    2016-01-01

    Intentional replantation is generally contraindicated in periodontally compromised teeth however, there are reports suggesting that it can be a successful treatment alternative for periodontally involved hopeless teeth. Currently there is dearth of evidence regarding the success of this therapy, especially evidence for the effectiveness of autologous platelet rich fibrin is lacking. We present a case report of a 23-year-old male patient with periodontally hopeless left maxillary central incisor having bone loss extending beyond root apex. The tooth was gently extracted and replanted utilizing root conditioning and combined regenerative therapy (Xenograft, PRF and Type I Collagen Membrane). Surgical re-entry at nine months revealed bone formation in the apical third of the tooth. At one year, 87% radiographic bone gain was accomplished. The improvement in the clinical and radiographic parameters reinforced by the re-entry surgery findings strongly suggest that intentional replantation may be a cost-effective substitute to implants and tooth supported prosthesis in situations where conventional periodontal therapy would yield compromised outcomes. PMID:27504421

  9. Platelet-rich fibrin matrix for facial plastic surgery.

    Science.gov (United States)

    Sclafani, Anthony P; Saman, Masoud

    2012-05-01

    Platelets are known primarily for their role in hemostasis, but there is increasing interest in the effect of platelets on wound healing. Platelet isolates such as platelet-rich plasma have been advocated to enhance and accelerate wound healing. This article describes the use of a novel preparation, platelet-rich fibrin matrix (PRFM), for facial plastic surgery applications such as volume augmentation, fat transfer supplementation, and as an adjunct to open surgical procedures.

  10. Fiber optic immunosensor for cross-linked fibrin concentration

    Science.gov (United States)

    Moskowitz, Samuel E.

    2000-08-01

    Working with calcium ions in the blood, platelets produce thromboplastin which transforms prothrombin into thrombin. Removing peptides, thrombin changes fibrinogen into fibrin. Cross-linked insoluble fibrin polymers are solubilized by enzyme plasmin found in blood plasma. Resulting D-dimers are elevated in patients with intravascular coagulation, deep venous thrombosis, pulmonary embolism, myocardial infarction, multiple trauma, cancer, impaired renal and liver functions, and sepsis. Consisting principally of a NIR 780 nm GaAlAs laser diode and a 800 nm avalanche photodiode (APD), the fiber-optic immunosensor can determined D-dimer concentration to levels detected by a second antibody which is labeled with NN 382 fluorescent dye. An evanescent wave traveling on an excitation optical fiber excites the antibody-antigen fluorophore complex. Concentration of cross-linked fibrin is directly proportional to the APD measured intensity of fluorescence. NIR fluorescence has advantages of low background interference, short fluorescence lifetime, and large difference between excitation and emission peaks. Competitive ELISA test for D-dimer concentration requires trained personnel performing a time consuming operation.

  11. An automated method for fibrin clot permeability assessment.

    Science.gov (United States)

    Ząbczyk, Michał; Piłat, Adam; Awsiuk, Magdalena; Undas, Anetta

    2015-01-01

    The fibrin clot permeability coefficient (Ks) is a useful measure of porosity of the fibrin network, which is determined by a number of genetic and environmental factors. Currently available methods to evaluate Ks are time-consuming, require constant supervision and provide only one parameter. We present an automated method in which drops are weighed individually, buffer is dosed by the pump and well defined clot washing is controlled by the software. The presence of a straight association between drop mass and their dripping time allows to shorten the measurement time twice. In 40 healthy individuals, Ks, the number of drops required to reach the plateau (DTP), the time to achieve the plateau (TTP) and the DTP/TTP ratio (DTR) were calculated. There was a positive association between Ks (r = 0.69, P Ks (r = -0.55, P Ks (r = 0.70, P < 0.0001 for the manual method and r = 0.76, P < 0.0001 for the automated method), fibrinogen (r = -0.58, P < 0.0001) and C-reactive protein (CRP) (r = -0.47, P < 0.01). The automated method might be a suitable tool for research and clinical use and may offer more additional parameters describing fibrin clot structure.

  12. A PEGylated Fibrin-Based Wound Dressing with Antimicrobial and Angiogenic Activity

    Science.gov (United States)

    2011-04-13

    CSM–PEGylated fibrin gel showing the uniform dispersion of SSD–CSM. (C) Rheology data shows that the PEGylated fibrin gel and SSD–CSM–PEGylated...and SSD–CSM in PEGylated fibrin gels (±SD) over a 72 h period (A) using a Franz apparatus or (B) under sink conditions. (C) Rheology data indicate...PEGylated fibrin gel Angiogenesis Adipose-derived stem cells a b s t r a c t Wounds sustained under battlefield conditions are considered to be contaminated

  13. From glue to gasoline: how competition turns perspective takers unethical.

    Science.gov (United States)

    Pierce, Jason R; Kilduff, Gavin J; Galinsky, Adam D; Sivanathan, Niro

    2013-10-01

    Perspective taking is often the glue that binds people together. However, we propose that in competitive contexts, perspective taking is akin to adding gasoline to a fire: It inflames already-aroused competitive impulses and leads people to protect themselves from the potentially insidious actions of their competitors. Overall, we suggest that perspective taking functions as a relational amplifier. In cooperative contexts, it creates the foundation for prosocial impulses, but in competitive contexts, it triggers hypercompetition, leading people to prophylactically engage in unethical behavior to prevent themselves from being exploited. The experiments reported here establish that perspective taking interacts with the relational context--cooperative or competitive--to predict unethical behavior, from using insidious negotiation tactics to materially deceiving one's partner to cheating on an anagram task. In the context of competition, perspective taking can pervert the age-old axiom "do unto others as you would have them do unto you" into "do unto others as you think they will try to do unto you."

  14. Autologous bone marrow transplantation by photodynamic therapy

    Science.gov (United States)

    Gulliya, Kirpal S.

    1992-06-01

    Simultaneous exposure of Merocyanine 540 dye containing cultured tumor cells to 514-nm laser light (93.6 J/cm2) results in virtually complete cell destruction. Under identical conditions, 40% of the normal progenitor (CFU-GM) cells survive the treatment. Laser- photoradiation treated, cultured breast cancer cells also were killed, and living tumor cells could not be detected by clonogenic assays or by anti-cytokeratin monoclonal antibody method. Thus, laser photoradiation therapy could be useful for purging of contaminating tumor cells from autologous bone marrow.

  15. Autologous chondrocyte implantation in children and adolescents

    DEFF Research Database (Denmark)

    Schmal, H; Pestka, J M; Salzmann, G

    2013-01-01

    PURPOSE: Autologous chondrocyte implantation (ACI) is a well-established treatment method for cartilage defects in knees. Age-related grouping was based on expression data of cartilage-specific markers. Specificities of ACI in the different populations were analysed. METHODS: Two hundred and sixt...... months after the operation. CONCLUSIONS: Age-related expression of cartilage-specific markers allows definition of adolescents in cartilage regenerating surgery. Chondromalacia in these patients is mainly caused by OCD or trauma. LEVEL OF EVIDENCE: Case series, Level IV....

  16. Does tranexamic acid stabilised fibrin support the osteogenic differentiation of human periosteum derived cells?

    Directory of Open Access Journals (Sweden)

    J Demol

    2011-03-01

    Full Text Available Fibrin sealants have long been used as carrier for osteogenic cells in bone regeneration. However, it has not been demonstrated whether fibrin’s role is limited to delivering cells to the bone defect or whether fibrin enhances osteogenesis. This study investigated fibrin’s influence on the behaviour of human periosteum-derived cells (hPDCs when cultured in vitro under osteogenic conditions in two-dimensional (fibrin substrate and three-dimensional (fibrin carrier environments. Tranexamic acid (TEA was used to reduce fibrin degradation after investigating its effect on hPDCs in monolayer culture on plastic.TEA did not affect proliferation nor calcium deposition of hPDCs under these conditions. Expression profiles of specific osteogenic markers were also maintained within the presence of TEA, apart from reduced alkaline phosphatase (ALP expression (day 14. Compared to plastic, proliferation was upregulated on 2D fibrin substrates with a 220% higher DNA content by day 21. Gene expression was also altered, with significantly (p<0.05 decreased Runx2 (day 7 and ALP (day 14 expression and increased collagen I expression (day 14 and 21. In contrast to plastic, mineralisation was absent on fibrin substrates. Inside fibrin carriers, hPDCs were uniformly distributed. Moderate cell growth and reduced osteogenic marker expression was observed inside fibrin carriers. After 2 weeks, increased cell death was present in the carrier’s centre. In conclusion, fibrin negatively influences osteogenic differentiation, compared to culture plastic, but enhanced proliferation (at least in 2D cultures for hPDCs cultured in osteogenic conditions. TEA maintained the integrity of fibrin-based constructs, with minor effects on the osteogenic differentiation of hPDCs.

  17. 纤维蛋白胶在右肝癌切除术中的应用%Application of fibrin glue in partial right hepatectomy for hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    郑进方; 王海东; 邢贻雷; 周开伦; 龙籍典

    2001-01-01

    目的观察纤维蛋白胶在右肝癌切除术中的止血效果。方法对21例右肝癌切除中应用纤维蛋白胶后的止血效果进行观察,并与未用纤维蛋白胶的对照组进行比较。结果使用纤维蛋白胶组术后24h平均失血160ml,对照组术后24h平均失血334ml(P<0.01)。结论纤维蛋白胶在右肝癌切除术中有良好的止血作用。

  18. 食管胃吻合口瘘窦道的封堵治疗%Application of fibrin glue in treating esophagogastric anastomotic leakage

    Institute of Scientific and Technical Information of China (English)

    何山红; 程金华; 闫明

    2004-01-01

    目的:探讨用生物蛋白胶封堵吻合口瘘窦道.方法:4例食管胃吻合口瘘均采用保守治疗,1例经颈部切口用手指钝性分离置管引流,其他3例分别在X线、B超、CT定位下穿刺引流,经内外冲洗感染灶治疗后,注射医用生物蛋白胶封堵瘘口窦道.结果:4例均在较短时间内治愈,无死亡发生,术后平均住院时间为32d,治疗效果满意.结论:穿刺引流避免了感染灶的扩大,生物蛋白胶封堵瘘口窦道明显缩短了病程,是行之有效的方法.

  19. 纤维蛋白胶的实验研究及临床应用%Fibrin Glue:the Experimental Study and Clinical Application

    Institute of Scientific and Technical Information of China (English)

    钱庆达; 汤漪凡; 李云芬

    1998-01-01

    本文讨论了纤维蛋白胶的配方、工具和使用方法,并报告了6例临床经验.纤维蛋白原浓度需>40mg/ml,凝血酶浓度>500U/ml能立即凝固,<50U/ml,凝固时间>60秒.可根据不同目的选用不同的工具和配方.抑肽酶并非必要成份.通过6例初步临应用总结使用时的方法及注意事项.

  20. Clinical application of fibrin glue in thyroid surgery%医用纤维蛋白胶在甲状腺手术中的应用

    Institute of Scientific and Technical Information of China (English)

    马茂; 范代娣

    2007-01-01

    目的 探讨医用纤维蛋白胶在甲状腺手术中的应用价值.方法 将79例甲状腺手术患者随机分成实验组(44例)和对照组(35例).实验组行甲状腺切除术,同时在手术创面喷洒医用纤维蛋白胶5 ml,对照组行甲状腺切除术,术中不用医用纤维蛋白胶,两组均放置引流管.观察两组术后总引流量、第1天引流量、拔管时间、伤口感染和拆线时间等情况.结果 实验组术后皮下总引流量、第1天引流量和拔管时间均少于对照组(P<0.05).两组间伤口感染发生率、拆线时间无明显差别(p>0.05).结论 应用医用纤维蛋白胶可有效地减少甲状腺术后创面渗液,促进创面愈合.

  1. Application of Fibrin Glue in Thoracic Surgery%纤维蛋白胶在胸外科手术中的应用

    Institute of Scientific and Technical Information of China (English)

    陈乾坤; 丁嘉安; 高文

    2004-01-01

    目的:了解纤维蛋白胶在胸外科手术中应用的效果.方法:在手术中将纤维蛋白胶喷至漏气、渗血、血管残端和支气管残端等处.结果:所有患者均未出现不良反应;术后平均漏气天数为0.14d,平均第1天引流量为273.5ml,平均第2天引流量为164.4ml,平均拔管时间为2.09d,术后胸管引流通畅未发现因蛋白胶脱落所致的胸管堵塞;所有患者均未发生术后局部感染;远期随访无支气管胸膜瘘发生.结论:纤维蛋白胶在胸外科领域有很好的应用前景.

  2. 纤维蛋白在软骨组织工程中的应用%Application of fibrin glue in cartilage tissue engineering

    Institute of Scientific and Technical Information of China (English)

    陈书军; 裴国献; 郭刚; 陈滨; 金丹; 杨志明

    2005-01-01

    纤维蛋白是纤维蛋白原经激活后形成的聚合物凝胶,作为软骨组织工程研究的载体材料,在相关的生物学和物理特性上具有其一定的优势.对纤维蛋白在软骨组织工程中应用的现状和发展方向进行综述.

  3. Study on Biologic Performance of Biological Fibrin Glue%医用生物蛋白胶生物性能的研究

    Institute of Scientific and Technical Information of China (English)

    苏怡; 候延文; 高珉之

    2001-01-01

    目的医用生物蛋白胶(简称BFG)应用到临床上具有实际意义.方法从细胞毒性、皮内刺激、皮肤致敏等生物性能对其进行安全性检测.结果无细胞毒性,对皮肤无刺激,亦无致敏反应.结论可以安全使用.

  4. Uncultured marrow mononuclear cells delivered within fibrin glue hydrogels to porous scaffolds enhance bone regeneration within critical-sized rat cranial defects.

    NARCIS (Netherlands)

    Kretlow, J.D.; Spicer, P.P.; Jansen, J.A.; Vacanti, C.A.; Kasper, F.K.; Mikos, A.G.

    2010-01-01

    For bone tissue engineering, the benefits of incorporating mesenchymal stem cells (MSCs) into porous scaffolds are well established. There is, however, little consensus on the effects of or need for MSC handling ex vivo. Culture and expansion of MSCs adds length and cost, and likely increases risk a

  5. Plerixafor for autologous CD34+ cell mobilization

    Directory of Open Access Journals (Sweden)

    Huda Salman

    2011-02-01

    Full Text Available Huda Salman, Hillard M LazarusDivision of Hematology-Oncology, Blood and Marrow Transplant Program, University Hospitals Case Medical Center, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine, Cleveland, OH, USAAbstract: High-dose chemotherapy and autologous transplantation of hematopoietic cells is a crucial treatment option for hematologic malignancy patients. Current mobilization regimes often do not provide adequate numbers of CD34+ cells. The chemokine receptor CXCR4 and ligand SDF-1 are integrally involved in homing and mobilization of hematopoietic progenitor cells. Disruption of the CXCR4/SDF-1 axis by the CXCR4 antagonist, plerixafor, has been demonstrated in Phase II and Phase III trials to improve mobilization when used in conjunction with granulocyte colony-stimulating factor (G-CSF. This approach is safe with few adverse events and produces significantly greater numbers of CD34+ cells when compared to G-CSF alone. New plerixafor initiatives include use in volunteer donors for allogeneic hematopoietic cell transplant and in other disease targets.Keywords: plerixafor, autologous hematopoietic cell transplant, CD34, lymphoma, myeloma, granulocyte colony-stimulating factor (G-CSF

  6. Anti-fibrin antibody binding in valvular vegetations and kidney lesions during experimental endocarditis.

    Science.gov (United States)

    Yokota, M; Basi, D L; Herzberg, M C; Meyer, M W

    2001-01-01

    In Streptococcus sanguinis (sanguis) induced experimental endocarditis, we sought evidence that the development of aortic valvular vegetation depends on the availability of fibrin. Endocarditis was induced in New Zealand white rabbits by catheter placement into the left ventricle and inoculation of the bacteria. Fibrin was localized in the developing vegetation with 99mTechnetium (Tc)-labeled anti-fibrin antibody one or three days later. When rabbit anti-fibrin antibody was given intravenously on day 1, the mass of aortic valvular vegetation was significantly reduced at day 3; infusion of non-specific rabbit IgG showed no effect. The 99mTc-labeled anti-fibrin antibody also labeled kidneys that showed macroscopic subcapsular hemorrhage. To learn if the deposition of fibrin in the kidneys was a consequence of endocarditis required a comparison of farm-bred and specific pathogen-free rabbits before and after the induction of endocarditis. Before induction, the kidneys of farm-bred rabbits were labeled, but specific pathogen-free rabbits were free of labeling and signs of macroscopic hemorrhage. After 3 days of endocarditis, kidneys of 10 of 14 specific pathogen-free rabbits labeled with 99mTc-labeled anti-fibrin antibody and showed hemorrhage. Kidney lesions were suggested to be a frequent sequellae of S. sanguinis infective endocarditis. For the first time, fibrin was shown to be required for the continued development of aortic valvular vegetations.

  7. Molecular weight fibrinogen variants determine angiogenesis rate in a fibrin matrix in vitro and in vivo

    NARCIS (Netherlands)

    Kaijzel, E.L.; Koolwijk, P.; Erck, M.G.M. van; Hinsbergh, V.W.M. van; Maat, M.P.M. de

    2006-01-01

    Background: During wound repair, fibrin acts both as a barrier to prevent blood loss and as a temporary matrix for the invasion and ingrowth of endothelial and tissue cells. A well-controlled angiogenesis process in the fibrinous exudate matrix is crucial for optimal wound healing. The composition a

  8. Identification of Quantitative Trait Loci for Fibrin Clot Phenotypes. The EuroCLOT Study

    DEFF Research Database (Denmark)

    Williams, Frances M K; Carter, Angela M; Kato, Bernet;

    2009-01-01

    associated with fibrin phenotypes. METHODS AND RESULTS: 447 dizygotic (DZ) and 460 monozygotic (MZ) pairs of healthy UK white female twins and 199 DZ twin pairs from Denmark were studied. D-dimer, an indicator of fibrin turnover, was measured by ELISA and measures of clot formation, morphology, and lysis...

  9. Improvement of fibrin clot structure after factor VIII injection in haemophilia A patients treated on demand.

    Science.gov (United States)

    Antovic, Aleksandra; Mikovic, Danijela; Elezovic, Ivo; Zabczyk, Michael; Hutenby, Kjell; Antovic, Jovan P

    2014-04-01

    Patients with haemophilia A have seriously impaired thrombin generation due to an inherited deficiency of factor (F)VIII, making them form unstable fibrin clots that are unable to maintain haemostasis. Data on fibrin structure in haemophilia patients remain limited. Fibrin permeability, assessed by a flow measurement technique, was investigated in plasma from 20 patients with severe haemophilia A treated on demand, before and 30 minutes after FVIII injection. The results were correlated with concentrations of fibrinogen, FVIII and thrombin-activatable fibrinolysis inhibitor (TAFI), and global haemostatic markers: endogenous thrombin potential (ETP) and overall haemostatic potential (OHP). Fibrin structure was visualised using scanning electron microscopy (SEM). The permeability coefficient Ks decreased significantly after FVIII treatment. Ks correlated significantly with FVIII levels and dosage, and with ETP, OHP and levels of TAFI. SEM images revealed irregular, porous fibrin clots composed of thick and short fibers before FVIII treatment. The clots had recovered after FVIII replacement almost to levels in control samples, revealing compact fibrin with smaller intrinsic pores. To the best of our knowledge, this is the first description of fibrin porosity and structure before and after FVIII treatment of selected haemophilia patients. It seems that thrombin generation is the main determinant of fibrin structure in haemophilic plasma.

  10. Influence of a constant magnetic field on the fibrinogen-fibrin system. [in blood coagulation process

    Science.gov (United States)

    Matskevichene, V. B.; Platonova, A. T.

    1974-01-01

    The effect of a constant magnetic field with a strength of 2500 oersteds on the fibrinogen-fibrin system was studied in the organism of healthy rabbits with exposure times of 1 and 5 hours. The results obtained indicate disruptions in the stage of conversion of fibrinogen to fibrin and an increase in the amount of fibrinogen.

  11. Modification of fibrin network ultrastructure by Fab fragments specific for different domain of fibrinogen.

    Science.gov (United States)

    Cierniewski, C S; Janiak, A; Wyroba, E

    1986-01-01

    Kinetics of inhibition of fibrin monomer polymerization produced by Fab fragments prepared from immunochemically purified monospecific antibodies to the surface epitopes of different domains of fibrinogen molecule has been correlated with electron microscopic observations of resulting specimens. Fab fragments prepared from anti FgD antisera were the most efficient inhibitors of thrombin-catalysed conversion of fibrinogen to fibrin; polymerization of fibrin monomers as detected spectrophotometrically was abolished at 2:1 molar ratio of anti FgD Fab fragments to fibra monomer. These Fab fragments acting as a steric hindrance of polymerization sites inhibited the first stage of fibrin monomer aggregation. Interaction of Fab fragments derived from antibodies specific for alpha 239-476 with corresponding segment of fibrinogen molecule resulted in a weak inhibition of fibrin monomer polymerization. However, fibrin obtained in the presence of these Fab fragments was significantly modified and showed no periodicity. This observation may suggest that anti alpha 239-476 Fab impaired the course of the second stage of fibrin monomer polymerization, i.e. lateral association of fibrin fibrils.

  12. Genetic predictors of fibrin D-dimer levels in healthy adults

    NARCIS (Netherlands)

    N.L. Smith (Nicholas); J.E. Huffman (Jennifer E.); D.P. Strachan (David); J. Huang (Jian); A. Dehghan (Abbas); S. Trompet (Stella); L.M. Lopez (Lorna M.); S.Y. Shin (So Youn); J. Baumert (Jens); V. Vitart (Veronique); J.C. Bis (Joshua); S.H. Wild (Sarah); A. Rumley (Ann); Q. Yang (Qiong Fang); A.G. Uitterlinden (André); D.J. Stott (David. J.); G. Davies (Gareth); A.M. Carter (Angela M.); B. Thorand (Barbara); O. Polašek (Ozren); B. McKnight (Barbara); H. Campbell (Harry); A.R. Rudnicka (Alicja); M.H. Chen (Min-hsin); B.M. Buckley (Brendan M.); S.E. Harris (Sarah); A. Peters (Annette); D. Pulanic (Drazen); T. Lumley (Thomas); A.J.M. de Craen (Anton J.M.); D.C. Liewald (David C.); C. Gieger (Christian); I. Ford (Ian); A.J. Gow (Alan J.); M. Luciano (Michelle); D.J. Porteous (David J.); X. Guo (Xiuqing); N. Sattar (Naveed); A. Tenesa (Albert); M. Cushman (Mary Ann); P.E. Slagboom (Eline); P.M. Visscher (Peter M.); T.D. Spector (Tim); T. Illig (Thomas); I. Rudan (Igor); E.G. Bovill (Edwin G.); A.F. Wright (Alan); W.L. McArdle (Wendy); G.H. Tofler (Geoffrey); A. Hofman (Albert); R.G.J. Westendorp (Rudi); J.M. Starr (John); P.J. Grant (Peter J.); M. Karakas (Mahir); N.D. Hastie (Nicholas D.); B.M. Psaty (Bruce); J.F. Wilson (James); G.D.O. Lowe (Gordon); C.J. O'Donnell (Christopher); J.C.M. Witteman (Jacqueline); J.W. Jukema (Jan Wouter); I.J. Deary (Ian); N. Soranzo (Nicole); W. Koenig (Wolfgang); C. Hayward (Caroline)

    2011-01-01

    textabstractBACKGROUND: Fibrin fragment D-dimer, one of several peptides produced when crosslinked fibrin is degraded by plasmin, is the most widely used clinical marker of activated blood coagulation. To identity genetic loci influencing D-dimer levels, we performed the first large-scale, genome-wi

  13. Ubiquitous distribution of salts and proteins in spider glue enhances spider silk adhesion

    Science.gov (United States)

    Amarpuri, Gaurav; Chaurasia, Vishal; Jain, Dharamdeep; Blackledge, Todd A.; Dhinojwala, Ali

    2015-03-01

    Modern orb-weaving spiders use micron-sized glue droplets on their viscid silk to retain prey in webs. A combination of low molecular weight salts and proteins makes the glue viscoelastic and humidity responsive in a way not easily achieved by synthetic adhesives. Optically, the glue droplet shows a heterogeneous structure, but the spatial arrangement of its chemical components is poorly understood. Here, we use optical and confocal Raman microscopy to show that salts and proteins are present ubiquitously throughout the droplet. The distribution of adhesive proteins in the peripheral region explains the superior prey capture performance of orb webs as it enables the entire surface area of the glue droplet to act as a site for prey capture. The presence of salts throughout the droplet explains the recent Solid-State NMR results that show salts directly facilitate protein mobility. Understanding the function of individual glue components and the role of the droplet's macro-structure can help in designing better synthetic adhesives for humid environments.

  14. Application of COMPONT Medical Adhesive Glue for Tension-Reduced Duraplasty in Decompressive Craniotomy

    Science.gov (United States)

    Zhou, Yujia; Wang, Gesheng; Liu, Jialin; Du, Yong; Wang, Lei; Wang, Xiaoyong

    2016-01-01

    Background The aim of this study was to evaluate the application of medical adhesive glue for tension-reduced duraplasty in decompressive craniotomy. Material/Methods A total of 56 cases were enrolled for this study from Jan 2013 to May 2015. All patients underwent decompressive craniotomy and the dura was repaired in all of them with tension-reduced duraplasty using the COMPONT medical adhesive to glue artificial dura together. The postoperative complications and the healing of dura mater were observed and recorded. Results No wound infection, epidural or subdural hematoma, cerebrospinal fluid leakage, or other complications associated with the procedure occurred, and there were no allergic reactions to the COMPONT medical adhesive glue. The second-phase surgery of cranioplasty was performed at 3 to 6 months after the decompressive craniotomy in 32 out of the 56 cases. During the cranioplasty we observed no adherence of the artificial dura mater patch to the skin flap, no residual COMPONT glue, or hydropic or contracture change of tissue at the surgical sites. Additionally, no defect or weakening of the adherence between the artificial dura mater patch and the self dura matter occurred. Conclusions COMPONT medical adhesive glue is a safe and reliable tool for tension-reduced duraplasty in decompressive craniotomy. PMID:27752035

  15. Tracing the biological origin of animal glues used in paintings through mitochondrial DNA analysis.

    Science.gov (United States)

    Albertini, Emidio; Raggi, Lorenzo; Vagnini, Manuela; Sassolini, Alessandro; Achilli, Alessandro; Marconi, Gianpiero; Cartechini, Laura; Veronesi, Fabio; Falcinelli, Mario; Brunetti, Brunetto Giovanni; Miliani, Costanza

    2011-03-01

    We report the development of a suitable protocol for the identification of the biological origin of binding media on tiny samples from ancient paintings, by exploitation of the high specificity and high sensitivity offered by the state-of-the art DNA analysis. In particular, our aim was to molecularly characterize mitochondrial regions of the animal species traditionally employed for obtaining glues. The model has been developed using aged painting models and then tested to analyze the organic components in samples from the polychrome terracotta Madonna of Citerna by Donatello (1415-1420), where, by GC-MS and FTIR spectroscopy, animal glues and siccative oils were identified. The results obtained are good in terms of both sensibility and specificity of the method. First of all, it was possible to confirm that Donatello used animal glue for the preparation of the painted layers of the Madonna of Citerna and, specifically, glue derived from Bos taurus. Data obtained from sequencing confirm that each sample contains animal glue, revealing that it was mostly prepared from two common European taurine lineages called T2 and T3. There is one remarkable exception represented by one sample which falls into a surviving lineage of the now extinct European aurochs.

  16. Platelet-rich fibrin application in dentistry: a literature review.

    Science.gov (United States)

    Borie, Eduardo; Oliví, Daniel García; Orsi, Iara Augusta; Garlet, Katia; Weber, Benjamín; Beltrán, Víctor; Fuentes, Ramón

    2015-01-01

    The development of bioactive surgical additives to regulate the inflammation and increase the speed of healing process is one of the great challenges in clinical research. In this sense, platelet rich fibrin (PRF) appears as a natural and satisfactory alternative with favorable results and low risks. The following review attempts to summarize the relevant literature regarding the technique of using PRF, focusing on its preparation, advantages, and disadvantages of using it in clinical applications. PRF alone or in combination with other biomaterials seems to have several advantages and indications both for medicine and dentistry, due it is a minimally invasive technique with low risks and satisfactory clinical results.

  17. Microbiology and pathology of fibrinous pericarditis in Danish slaughter pigs

    DEFF Research Database (Denmark)

    Buttenschøn, J.; Friis, N.F.; Aalbaek, B.

    1997-01-01

    The prevalence of fibrinous pericarditis detected at slaughter in Danish slaughter pigs is approximately 0.02 %. The microbiology and pathology of this disorder was studied through 46 field cases collected at slaughter from May 1994 to August 1995. Mycoplasmas (Mycoplasma hyopneumoniae, M...... from the pericardium in three cases. The pericardium was sterile in three cases and contained a low grade mixed flora in two; the latter is believed to be a result of the slaughter procedure rather than an infection. No chlamydiae were found in connection with the microbiological examination...

  18. Revitalization of traumatized immature tooth with platelet-rich fibrin

    Directory of Open Access Journals (Sweden)

    Umrana Faizuddin

    2015-01-01

    Full Text Available Endodontic treatment options for immature, nonvital teeth conventionally include surgical endodontics, apexification with calcium hydroxide, or single visit mineral trioxide aggregate plug. Regeneration is a new concept which is been introduced in the treatment of traumatized open apex tooth. Regeneration of pulp-dentin complex in an infected necrotic tooth with an open apex is possible if the canal is effectively disinfected. The purpose of this case report is to add a new vista in regenerative, endodontic therapy by using platelet-rich fibrin for revitalization of immature nonvital tooth.

  19. Computational imaging analysis of fibrin matrices with the inclusion of erythrocytes from homozygous SS blood reveals agglomerated and amorphous structures.

    Science.gov (United States)

    Averett, Rodney D; Norton, David G; Fan, Natalie K; Platt, Manu O

    2017-01-01

    Sickle cell disease is a single point mutation disease that is known to alter the coagulation system, leading to hypercoagulable plasma conditions. These hypercoagulable conditions can lead to complications in the vasculature, caused by fibrin clots that form undesirably. There is a need to understand the morphology and structure of fibrin clots from patients with sickle cell disease, as this could lead to further discovery of treatments and life-saving therapies. In this work, a computational imaging analysis method is presented to evaluate fibrin agglomeration in the presence of erythrocytes (RBCs) homozygous for the sickle cell mutation (SS). Numerical algorithms were used to determine agglomeration of fibrin fibers within a matrix with SS RBCs to test the hypothesis that fibrin matrices with the inclusion of SS RBCs possess a more agglomerated structure than native fibrin matrices with AA RBCs. The numerical results showed that fibrin structures with SS RBCs displayed an overall higher degree of agglomeration as compared to native fibrin structures. The computational algorithm was also used to evaluate fibrin fiber overlap (aggregation) and anisotropy (orientation) in normal fibrin matrices compared to fibrin matrices polymerized around SS RBCs; however, there was no statistical difference. Ultrasound measurements of stiffness revealed rigid RBCs in the case of samples derived from homozygous SS blood, and densely evolving matrices, when compared to normal fibrin with the inclusion of AA RBCs. An agglomeration model is suggested to quantify the fibrin aggregation/clustering near RBCs for both normal fibrin matrices and for the altered structures. The results of this work are important in the sense that the understanding of aggregation and morphology in fibrin clots with incorporation of RBCs from persons living with sickle cell anemia may elucidate the complexities of comorbidities and other disease complications.

  20. Application of the silicon photomultipliers for detectors in the GlueX experiment

    Energy Technology Data Exchange (ETDEWEB)

    Somov, Sergey V. [National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Kashirskoe shosse 31, Moscow, 115409, Russia; Tolstukhin, Ivan [National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Kashirskoe shosse 31, Moscow, 115409, Russia; Somov, Alexander S. [Jefferson Lab, Newport News, VA

    2015-11-01

    The GlueX detector in Hall D at Jefferson Lab is instrumented with about 5000 Silicon Photomultipliers (SiPM) manufactured by Hamamatsu Corporation [2]. These photo sensors have properties similar to conventional photomultipliers but can be operated at high magnetic fields. Silicon photomultipliers with a sensitive area of 3x3 mm2 are used to detect light from the following GlueX scintillator detectors: the tagger microscope, pair spectrometer, and start counter. Arrays of 4x4 SiPMs sensors were chosen for the instrumentation of the barrel electromagnetic calorimeter. The tagger microscope must operate at high rates (up to 2.5 MHz) and provide time measurements with a resolution better than 0.3 ns. The paper will describe some results of the characterization of SiPMs for various GlueX sub-detectors.

  1. Determination of relationships between density, amount of glue and mechanical properties of OSB

    Directory of Open Access Journals (Sweden)

    Jaroslav Hrázský

    2009-03-01

    Full Text Available This paper deals with problems of the effect of changes in density and amount of glue on mechanical properties of OSB (oriented strand boards. Tests of mechanical properties were carried out on OSB/3, 18 and 15 mm thick, produced on an OSB production line of the prominent manufacturer of board composite materials in the Czech Republic. In the first stage of the experiment, density was decreased in manufactured OSB with a nearly the same amount of glue. In the second stage, density was constant and the amount of glue changed. Bending strength, modulus of elasticity and tensile strength perpendicular to the board surface were determined in OSB samples of particular variants. Results of laboratory tests were compared with values given by the ČSN EN 300 standard for category OSB/3.

  2. Exotic Hybrid Meson Spectroscopy with the GlueX detector at Jlab

    Energy Technology Data Exchange (ETDEWEB)

    Lawrence, David W. [JLAB

    2014-03-01

    The GlueX experiment is scheduled to begin taking data in 2015. The goal is to discover evidence for the existence of exotic hybrid mesons and to map out their spectrum in the light quark sector. Recent theoretical developments using Lattice QCD predict exotic hybrid states in a mass range accessible using the newly upgraded 12GeV electron accelerator at Jefferson Lab. Hybrid mesons, and in particular exotic hybrid mesons, provide the ideal laboratory for testing QCD in the confinement regime since these mesons explicitly manifest the gluonic degrees of freedom. The experiment will use 9 GeV linearly polarized photons produced via coherent bremsstrahlung to produce the exotic hybrids. The decay products will be detected in the solenoid-based GlueX detector currently under construction at Jefferson Lab. The status of the GlueX experiment including detector parameters will be presented along with theoretical motivation for the experiment.

  3. Autologous Stem Cell Transplant for AL Amyloidosis

    Directory of Open Access Journals (Sweden)

    Vivek Roy

    2012-01-01

    Full Text Available AL amyloidosis is caused by clonal plasma cells that produce immunoglobulin light chains which misfold and get deposited as amyloid fibrils. Therapy directed against the plasma cell clone leads to clinical benefit. Melphalan and corticosteroids have been the mainstay of treatment for a number of years and the recent availability of other effective agents (IMiDs and proteasome inhibitors has increased treatment options. Autologous stem cell transplant (ASCT has been used in the treatment of AL amyloidosis for many years. It is associated with high rates of hematologic response and improvement in organ function. However, transplant carries considerable risks. Careful patient selection is important to minimize transplant related morbidity and mortality and ensure optimal patient outcomes. As newer more affective therapies become available the role and timing of ASCT in the overall treatment strategy of AL amyloidosis will need to be continually reassessed.

  4. Nuovi Metodi di visualizzazione geografica: l'approccio Focus+Glue+Context

    Directory of Open Access Journals (Sweden)

    Flavio Lupia

    2010-03-01

    Full Text Available New cartographic visualization methods:the Focus+Glue+Context approachFocus+Glue+Context is a new cartographic visualization method specifically designed to solve the fruition problems connected with the use of mobile devices and web mapping services. The objective of the F+G+C approach is to reduce users cognitive efforts when reading a map: to do so, the area of interest is ‘highlighted’ in a lower and more detailed scale through a fisheye lens effect, while the sorrouding context, useful to the user to determine the items relationships in a map, is maintained on a higher scale.

  5. Glue from the Sea:Biomedical Adhesives Inspired by Algal Polymers

    Institute of Scientific and Technical Information of China (English)

    H.Bianco-Peled; R.Bitton; P.Potin

    2007-01-01

    1 Introduction Tissue repair following surgery or trauma has been dominated by sutures,staples and wiring.Although these techniques are well established and widely used,their application often involves pain,unaesthetic results,or bleeding. These limitations emphasize the need for adhesive products to be available to surgeons.2 ResultsA challenging aspect of developing new tissue adhesive is to create a material that can glue wet surfaces.The success of synthetic glues under such an environment is very ...

  6. Effect of polylactic acid glue in preventing epidural scar adhesion after laminectomy in rabbits

    Institute of Scientific and Technical Information of China (English)

    LIU Li-min; SONG Yue-ming; DUAN Hong; DING Yong-li; LU Bing

    2006-01-01

    Objective: To determine the efficacy of polylactic acid glue in preventing epidural scar adhesion after laminectomy in rabbits.Methods: Twenty-four apanese white rabbits underwent laminectomy (including the attached ligaments)at L2 and Ls. After laminectomy at L5, polylactic acid glue was sprayed on the dura and nerve roots and this segment was taken as the experimental group. After laminectomy at L2 , nothing was used and this segment was enrolled as the self control group. Four rabbits were killed every two weeks postoperatively till the end of the experiment at 12weeks. Then the operated spine was observed grossly,histologically and ultrastructurally to check the degree of scar formation, the status of epidural scar adhesion, the absorption of the glue, and the intracellular structure of fibroblasts.Results: The glue coagulated immediately after spraying and showed excellent hemostatic effect. The glue membrane was easy to be taken away from the dura mater of the samples for 2 weeks and there were no cells in the epidural space in the experimental group. But the dura mater was covered by hematoma in the control group,which formed mild adhesion, with fibroblasts proliferating actively. In the 4th week, some glue shivers remained in the epidural space with fibroblasts increasing a little, and the dura mater was smooth in the experimental group.However, in the control group, the formed scar was fragile and conglutinated with the dura mater diffusely and fibroblasts were much more than those in the experimental group. In the 6th-12th weeks, there was a potential interspace between the scar and the dura mater, and the polylactic acid glue was absorbed completely in the experimental group. Much tough scar was found in the control group, which was very difficult to dissect from the dura mater and the surrounding tissues. From the ultrastructural observation of the fibroblasts, the nucleus became much bigger and the rough endoplasmic reticulum was much more plentiful in

  7. Soft fibrin gels promote selection and growth of tumorigenic cells

    Science.gov (United States)

    Liu, Jing; Tan, Youhua; Zhang, Huafeng; Zhang, Yi; Xu, Pingwei; Chen, Junwei; Poh, Yeh-Chuin; Tang, Ke; Wang, Ning; Huang, Bo

    2012-08-01

    The identification of stem-cell-like cancer cells through conventional methods that depend on stem cell markers is often unreliable. We developed a mechanical method for selecting tumorigenic cells by culturing single cancer cells in fibrin matrices of ~100 Pa in stiffness. When cultured within these gels, primary human cancer cells or single cancer cells from mouse or human cancer cell lines grew within a few days into individual round colonies that resembled embryonic stem cell colonies. Subcutaneous or intravenous injection of 10 or 100 fibrin-cultured cells in syngeneic or severe combined immunodeficiency mice led to the formation of solid tumours at the site of injection or at the distant lung organ much more efficiently than control cancer cells selected using conventional surface marker methods or cultured on conventional rigid dishes or on soft gels. Remarkably, as few as ten such cells were able to survive and form tumours in the lungs of wild-type non-syngeneic mice.

  8. Fibrin formation and dissolution in women receiving oral contraceptive drugs.

    Science.gov (United States)

    Ball, A P; McKee, P A

    1977-04-01

    Factors affecting fibrin formation and dissolution were compared for 15 women taking combined oral contraceptives and 15 women using nonpharmacological methods of birth control. The two groups were matched for age, body weight, time of blood collection, and day in menstrual cycle; none of the women was receiving other drugs known to affect the blood coagulation or fibrinolytic parameters measured in this study. Fibrinogen concentrations tended to be higher in the experimental group; the degree of fibrinogen degradation, number of fibrin cross-links, and levels of factor XIII and plasminogen were the same for both group. There were significant reductions in antithrombin activity, the euglobulin lysis time, and fibrinolytic inhibitor level in women using oral contraceptives. An estrogen dose effect was suggested for fibrinogen concentration and the degree of antithrombin activity. The increased fibrinolytic activity and decreased fibrinolytic inhibitor levels are consistent with in vitro observations that antithrombin also inhibits plasmin activity. Thus while oral contraceptive-induced depression of antithrombin III could possibly predispose to thrombosis by diminishing the inhibition of the serine protease clotting factors, the concomitant decreased level of plasmin inhibition might balance the system by favoring thrombolysis as well as the digestion and inactivation of certain clotting factors by plasmin.

  9. Genipin-crosslinked fibrin hydrogels as a potential adhesive to augment intervertebral disc annulus repair.

    Science.gov (United States)

    Schek, R M; Michalek, A J; Iatridis, J C

    2011-04-18

    Treatment of damaged intervertebral discs is a significant clinical problem and, despite advances in the repair and replacement of the nucleus pulposus, there are few effective strategies to restore defects in the annulus fibrosus. An annular repair material should meet three specifications: have a modulus similar to the native annulus tissue, support the growth of disc cells, and maintain adhesion to tissue under physiological strain levels. We hypothesized that a genipin crosslinked fibrin gel could meet these requirements. Our mechanical results showed that genipin crosslinked fibrin gels could be created with a modulus in the range of native annular tissue. We also demonstrated that this material is compatible with the in vitro growth of human disc cells, when genipin:fibrin ratios were 0.25:1 or less, although cell proliferation was slower and cell morphology more rounded than for fibrin alone. Finally, lap tests were performed to evaluate adhesion between fibrin gels and pieces of annular tissue. Specimens created without genipin had poor handling properties and readily delaminated, while genipin crosslinked fibrin gels remained adhered to the tissue pieces at strains exceeding physiological levels and failed at 15-30%. This study demonstrated that genipin crosslinked fibrin gels show promise as a gap-filling adhesive biomaterial with tunable material properties, yet the slow cell proliferation suggests this biomaterial may be best suited as a sealant for small annulus fibrosus defects or as an adhesive to augment large annulus repairs. Future studies will evaluate degradation rate, fatigue behaviors, and long-term biocompatibility.

  10. Fibrin/platelet plug counteracts cutaneous wound contraction: the hypothesis of "skipping stone".

    Science.gov (United States)

    Farahani, Ramin Mostofi Zadeh

    2007-01-01

    Cutaneous wound contraction and epithelialization act collaboratively to minimize the exposed wound surface. However excessive wound contraction is undesirable due to the resultant disfigurement and scarring. Fibrin clot has greater stiffness than surrounding tissue and mechanical strain further enhances its stiffness. On the contrary, skin exhibits diminished stiffness when affected by high strain rates. Therefore during early stages of wound healing, the contractile wound border is confronted by fibrin clot forming a high strain region in the interface of contractile tissue and fibrin clot--which is evidenced by computer simulation. Due to the stress relaxation property of skin, the contractile strain is partly neutralized. Meanwhile, gradually the stiffness of fibrin clot decreases which is followed by another cycle of wound contraction. This cyclic pattern of contraction resembles the movement of a stone over water or "skipping stone". The stone bounces repeatedly when thrown across the surface of water with reduction of jumping altitude with each bounce till the stone stops completely. This hypothesis is further supported by the observed initial delay in wound contraction and the chronological correlation of enhanced wound contraction with loss of superficial eschar and substitution of fibrin clot with granulation tissue. Also there is evidence that fibrin inhibits fibroblast-mediated contraction of collagen. Furthermore, modest increase in wound contraction rate in fibrinogen deficient mice and fibrin-mediated diminished wound contraction are agreement with the proposed hypothesis.

  11. Fibronectin provides a conduit for fibroblast transmigration from collagenous stroma into fibrin clot provisional matrix.

    Science.gov (United States)

    Greiling, D; Clark, R A

    1997-04-01

    After injury, the wound space is filled with a fibrin/fibronectin clot containing growth factors released by platelets and monocytes. In response to these factors, fibroblasts migrate into the fibrin clot and contribute to the formation of granulation tissue. The functional mechanisms allowing fibroblasts to leave the collagenous matrix of normal connective tissue and invade the provisional matrix of the fibrin clot have not been fully defined. To investigate these mechanisms we established a new in vitro model which simulates specific aspects of early wound healing, that is, the migration of fibroblasts from a three-dimensional collagen matrix into a fibrin clot. This transmigration could be induced by physiological concentrations of platelet releasate or platelet-derived growth factor BB (PDGF-BB) in a concentration-dependent manner. At 24 hours irradiated fibroblasts invaded the fibrin gel almost as well as non-irradiated cells, indicating that transmigration was independent of proliferation. Plasminogen and its activators appear to be necessary for invasion of the fibrin clot since protease inhibitors decreased the amount of migration. These serine proteases, however, were not necessary for exit from the collagen gel as fibroblasts migrated out of the collagen gel onto a surface coated with fibrin fibrils even in the presence of inhibitors. Removal of fibronectin (FN) from either the collagen gel or the fibrin gel markedly decreased the number of migrating cells, suggesting that FN provides a conduit for transmigration. Cell movement in the in vitro model was inhibited by RGD peptide, and by monoclonal antibodies against the subunits of the alpha5 beta1 and alpha v beta3 integrin receptor. Thus, the functional requirements for fibroblast transmigration from collagen-rich to fibrin-rich matrices, such as occurs in early wound healing, have been partially defined using an in vitro paradigm of this important biologic process.

  12. Venous gas embolism caused by fibrin sealant application to the prostate during greenlight laser photoselective vaporization.

    Science.gov (United States)

    Lee, Alexander; Vazquez, Rafael

    2015-04-15

    Venous gas embolism is a complication of fibrin sealant application and is a well-described event during various modes of prostate resection. We describe the case of a nitrogen venous gas embolism during Greenlight laser photovaporization of the prostate during the application of fibrin sealant to the operative site for hemostasis. Fibrin sealant application by a compressed gas applicator is a cause of venous air embolism, and this case highlights the need to keep venous gas embolism in mind when compressed gas applicators are used.

  13. The Platelet Integrin αIIbβ3 Differentially Interacts with Fibrin Versus Fibrinogen.

    Science.gov (United States)

    Litvinov, Rustem I; Farrell, David H; Weisel, John W; Bennett, Joel S

    2016-04-08

    Fibrinogen binding to the integrin αIIbβ3 mediates platelet aggregation and spreading on fibrinogen-coated surfaces. However,in vivoαIIbβ3 activation and fibrinogen conversion to fibrin occur simultaneously, although the relative contributions of fibrinogenversusfibrin to αIIbβ3-mediated platelet functions are unknown. Here, we compared the interaction of αIIbβ3 with fibrin and fibrinogen to explore their differential effects. A microscopic bead coated with fibrinogen or monomeric fibrin produced by treating the immobilized fibrinogen with thrombin was captured by a laser beam and repeatedly brought into contact with surface-attached purified αIIbβ3. When αIIbβ3-ligand complexes were detected, the rupture forces were measured and displayed as force histograms. Monomeric fibrin displayed a higher probability of interacting with αIIbβ3 and a greater binding strength. αIIbβ3-fibrin interactions were also less sensitive to inhibition by abciximab and eptifibatide. Both fibrinogen- and fibrin-αIIbβ3 interactions were partially inhibited by RGD peptides, suggesting the existence of common RGD-containing binding motifs. This assumption was supported using the fibrin variants αD97E or αD574E with mutated RGD motifs. Fibrin made from a fibrinogen γ'/γ' variant lacking the γC αIIbβ3-binding motif was more reactive with αIIbβ3 than the parent fibrinogen. These results demonstrate that fibrin is more reactive with αIIbβ3 than fibrinogen. Fibrin is also less sensitive to αIIbβ3 inhibitors, suggesting that fibrin and fibrinogen have distinct binding requirements. In particular, the maintenance of αIIbβ3 binding activity in the absence of the γC-dodecapeptide and the α-chain RGD sequences suggests that the αIIbβ3-binding sites in fibrin are not confined to its known γ-chain and RGD motifs.

  14. Morphological Study of Isolated Ovarian Preantral Follicles Using Fibrin Gel Plus Platelet Lysate after Subcutaneous Transplantation

    Directory of Open Access Journals (Sweden)

    Ali Reza Rajabzadeh

    2015-04-01

    Full Text Available Objective: Ovarian and follicle transplantation may preserve fertility in young cancer survivors. In this study, we have transplanted preantral follicles using fibrin gel as a carrier and fibrin gel supplemented with platelet lysate (PL as a rich source of angiogenic and growth factors. The purpose of this study was to evaluate the role of fibrin gel and PL in follicle transplantation. Materials and Methods: In this experimental study, ovaries were taken from 14-dayold Naval Medical Research Institute (NMRI mice. Preantral follicles were dissected from the ovaries and encapsulated into fibrin gel supplemented with 5, 10, 15 or 20% PL, then transplanted back into the same donor mice. Fibrin gels supplemented with PL that contained preantral follicles were placed in a subcutaneous pocket in the back of the neck of the recipient, donor mouse (the same mouse that follicles were collected. After 14 days the grafts were processed and embedded in paraffin blocks, then serially sectioned for histological evaluation. We counted the follicles and classified them according to stage (preantral or antral. Data were presented as mean ± standard error of mean (SEM and analysed by analysis of variance (ANOVA and the Kruskal-Wallistest. Results: The mean percentage of recovered follicles encapsulated and transplanted in each group were 33.30 ± 2.47 (fibrin gel, 31.96 ± 1.90 (fibrin gel+5% PL, 34.02 ± 2.44 (fibrin gel+10% PL, 48.31 ± 2.06 (fibrin gel+15% PL and 17.60 ± 2.79 (fibrin gel+20% PL. There was a significant increase in the recovery rate of grafted follicles with fibrin gel+15% PL (48.31%; p<0.001. The percentage of preantral follicles showed no significant difference in all groups (p<0.05. The percentage of antral follicles showed a significant decrease in follicles grafted with fibrin gel+20% PL when compared to the other groups (11.77%; p<0.005 but no significant difference was observed in the other groups. Conclusion: The use of PL in follicle

  15. 富血小板纤维蛋白新生诱导骨的组织学观察%Histological observation on new bone induced by platelet-rich fibrin

    Institute of Scientific and Technical Information of China (English)

    付冬梅; 肖琼; 杨琴秋; 董露; 陈红亮; 孙勇

    2016-01-01

    BACKGROUND:In previous experiments, we have confirmed that platelet rich fibrin has the ability of osteoinduction, and have conducted a preliminary study on its microstructure and biomechanics. However, little is reported on its histology research. OBJECTIVE:To compare the histological changes after implanting platelet-rich fibrin, Bio-Oss and autologous bone and to analyze the pros and cons of platelet-rich fibrin implantation for repair of bone defects. METHODS: As previously reported, animal models of critical bone defects were established respectively on the bilateral femoral condyles of 12 beagle dogs. Then, platelet-rich fibrin, Bio-Oss+colagen membrane (Bio-Oss group) and autologous bone (autologous bone group)+colagen membrane were respectively implanted. At 3, 6, 8 and 12 months, one experimental dog from each group was kiled, respectively, and histological observation was performed. Another beagle dog as blank control was enroled to establish the animal model of critical bone defects, with no implantation. RESULTS AND CONCLUSION:At 3, 6, 8 and 12 months after implantation, there were significant differences in the new bone formation speed and amount between the platelet-rich fibrin group, Bio-Oss group and autologous bone group. These three kinds of bone grafts al had osteoinductive ability to different extents. In the platelet-rich fibrin group, the osteogenic effects were better at 3 and 6 months, and the new bone was similar to natural one; in the autologuos bone group, bone necrosis was noticeable at 3 and 6 months, but the osteogenic effects became better at 8 months, and the new bone was similar to natural one at 12 months; in the Bio-oss group, the osteogenic effects were similar to those in the platelet-rich fibrin group, but the residual of Bio-oss was visible at 12 months; in the blank control group, no bone formed at 3 months, indicating the animal model of critical bone defects was made successfuly. In brief, the platelet-rich fibrin has good

  16. The combination use of platelet-rich fibrin and treated dentin matrix for tooth root regeneration by cell homing.

    Science.gov (United States)

    Ji, Baohui; Sheng, Lei; Chen, Gang; Guo, Shujuan; Xie, Li; Yang, Bo; Guo, Weihua; Tian, Weidong

    2015-01-01

    Endogenous regeneration through cell homing provides an alternative approach for tissue regeneration, except cell transplantation, especially considering clinical translation. However, tooth root regeneration through cell homing remains a provocative approach in need of intensive study. Both platelet-rich fibrin (PRF) and treated dentin matrix (TDM) are warehouses of various growth factors, which can promote cell homing. We hypothesized that endogenous stem cells are able to sense biological cues from PRF membrane and TDM, and contribute to the regeneration of tooth root, including soft and hard periodontal tissues. Therefore, the biological effects of canine PRF and TDM on periodontal ligament stem cells (PDLSCs) and bone marrow mesenchymal stem cells (BMSCs) were evaluated respectively in vitro. Beagle dogs were used as orthotopic transplantation model. It was found that PRF significantly recruited and stimulated the proliferation of PDLSCs and BMSCs in vitro. Together, PRF and TDM induced cell differentiation by upregulating the mineralization-related gene expression of bone sialoprotein (BSP) and osteopotin (OPN) after 7 days coculture. In vivo, transplantation of autologous PRF and allogeneic TDM into fresh tooth extraction socket achieved successful root regeneration 3 months postsurgery, characterized by the regeneration of cementum and periodontal ligament (PDL)-like tissues with orientated fibers, indicative of functional restoration. The results suggest that tooth root connected to the alveolar bone by cementum-PDL complex can be regenerated through the implantation of PRF and TDM in a tooth socket microenvironment, probably by homing of BMSCs and PDLSCs. Furthermore, bioactive cues and inductive microenvironment are key factors for endogenous regeneration. This approach provides a tangible pathway toward clinical translation.

  17. Effect of Platelet Rich Plasma and Platelet Rich Fibrin on sciatic nerve regeneration in a rat model.

    Science.gov (United States)

    Lichtenfels, Martina; Colomé, Lucas; Sebben, Alessandra Deise; Braga-Silva, Jefferson

    2013-07-01

    The aim of this study was to evaluate the effect of Platelet Rich Plasma (PRP) and Platelet Rich Fibrin (PRF) on peripheral nerve repair. Thirty-two Wistar rats were randomly divided into four equal treatments groups: autologous nerve grafts (ANG), silicon tube plus saline solution (SS), silicon tube plus PRP, and silicon tube plus PRF. In ANG group, 10 mm segment from sciatic nerve was excised and reimplanted between the nerve stumps. In the SS, PRP, and PRF groups, 5 mm segment from sciatic nerve was excised and bridged with a 12 mm silicone conduit to create a 10 mm nerve gap. The conduit was filled in accordance with the different treatments. Walking track analysis was performed periodically and on the 90th post-operative day histomorphometric analysis was performed. The ANG, PRF, and PRP groups presented a significant functional improvement in relation to the SS group (P = 0.001) on 90 days after surgery. Histomorphometric analysis demonstrated that the ANG group achieved a larger nerve fiber diameter in proximal stump while comparing with the SS group (P =0.037) and showed larger fiber diameter in median stump in comparison to the PRP group (P = 0.002) and PRF group (P = 0.001). Axonal diameter and myelin sheath thickness showed no statistical significant difference between the groups in the three stumps (P ≥ 0.05). This study suggests that PRP and PRF have positive effects on the functional nerve recovery; however, these groups don't achieve a significant improvement on the histomorphometric analysis.

  18. Comparative uncertainty analysis of copper loads in stormwater systems using GLUE and grey-box modeling

    DEFF Research Database (Denmark)

    Lindblom, Erik Ulfson; Madsen, Henrik; Mikkelsen, Peter Steen

    2007-01-01

    . With the proposed model and input data, the GLUE analysis show that the total sampled copper mass can be predicted within a range of +/- 50% of the median value ( 385 g), whereas the grey-box analysis showed a prediction uncertainty of less than +/- 30%. Future work will clarify the pros and cons of the two methods...

  19. Degradation mechanism of silicone glues under UV irradiation and options for designing materials with increased stability

    NARCIS (Netherlands)

    Fischer, H.R.; Semprimoschnig, C.; Mooney, C.; Rohr, T.; Eck, E.R.H. van; Verkuijlen, M.H.W.

    2013-01-01

    The degradation of silicone glues used, for example, in the assembly of solar modules for use in space, has been investigated and possible mechanisms which lead to colouration and possible embrittlement are analysed. Both effects are connected to the generation of radicals upon exposure to UV irradi

  20. Observations on dimensional changes of sized canvas based on glue temperature

    DEFF Research Database (Denmark)

    Krarup Andersen, Cecil

    2008-01-01

    The aim of this study was to explore dimensional changes caused by water on sized canvas. Samples of new linen canvas were mounted on a rig for biaxial tensioning, holding a constant stress of 100 N/m in both weave directions. The samples were then sized with respectively warm fluent glue (45 °C...

  1. Note: Effects of several thermal glues used on temperature dependent Hall measurements.

    Science.gov (United States)

    Rangel-Kuoppa, Victor-Tapio; Chen, Gang

    2010-03-01

    The effects of four thermal glues (cry-con, fixogum, RS 503-357, and silicon-high vacuum-grease from Leybold vacuum) on temperature dependent Hall measurements on n-type silicon are tested. All thermal glues yielded the same results (resistivity, mobility, and charge carrier density) between 300 and 190 K. The use of RS 503-357 drastically distorts the expected results below 190 K, probably due to a phase transition and its latent heat, which affects the sample temperature during the phase transition. All the other thermal glues give reproducible results down to 100 K. Below 100 K, the use of cry-con, fixogum, and the silicon-high vacuum-grease from Leybold vacuum yield decreasing mobility and charge carrier density and increasing resistivity, as temperature decreases, but with different magnitudes. This is explained as the thermal properties of each glue start to diverge. Fixogum seems to give the best thermal conductivity, while the silicon-high vacuum grease from Leybold vacuum performs the worst below 100K. Crycon has an intermediate behavior between these two former ones. Cooling speed plays an important role at these low temperatures.

  2. Study of glue extrusion after endoscopic N-butyl-2-cyanoacrylate injection on gastric variceal bleeding

    Institute of Scientific and Technical Information of China (English)

    Yan-Mei Wang; Liu-Fang Cheng; Nan Li; Kai Wu; Jun-Shan Zhai; Ya-Wen Wang

    2009-01-01

    AIM: To investigate glue extrusion after endoscopic N-butyl-2-cyanoacrylate injection on gastric variceal bleeding and to evaluate the long-term efficacy and safety of this therapy.METHODS: A total of 148 cirrhotic patients in our hospital with esophagogastric variceal bleeding (EGVB) were included in this study. N-butyl-2-cyanoacrylate was mixed with lipiodol in a 1:1 ratio and injected as a bolus of 1-3 mL according to variceal size. Patients underwent endoscopic follow-up the next week, fourth week, second month, fourth month, and seventh month after injection and then every 6 mo to determine the cast shape. An abdominal X-ray film and ultrasound or computed tomographic scan were also carried out in order to evaluate the time of variceal disappearance and complete extrusion of the cast. The average follow-up time was 13.1 mo.RESULTS: The instantaneous hemostatic rate was 96.2%. Early re-bleeding after injection in 9 cases (6.2%) was estimated from rejection of adhesive. Late re-bleeding occurred in 12 patients (8.1%) at 2-18 mo. The glue cast was extruded into the lumen within one month in 86.1% of patients and eliminated within one year. Light erosion was seen at the injection position and mucosa edema in the second week. The glue casts were extruded in 18 patients (12.1%) after one week and in 64 patients (42.8%) after two weeks. All kinds of glue clumping shapes and colors on endoscopic examination were observed in 127 patients (86.1%) within one month, including punctiform, globular, pillar and variform. Forty one patients (27.9%) had glue extrusion after 3 mo and 28 patients (28.9%) after six months. The extrusion time was not related to the injection volume of histoacryl. Obliteration was seen in 70.2% (104 cases) endoscopically. The main complication was re-bleeding resulting from extrusion. The prognosis of the patients depended on the severity of the underlying liver disease.CONCLUSION: Endoscopic injection of cyanoacrylate is highly effective for

  3. Potential dental pulp revascularization and odonto-/osteogenic capacity of a novel transplant combined with dental pulp stem cells and platelet-rich fibrin.

    Science.gov (United States)

    Chen, Yong-Jin; Zhao, Yin-Hua; Zhao, Ya-Juan; Liu, Nan-Xia; Lv, Xin; Li, Qiang; Chen, Fa-Ming; Zhang, Min

    2015-08-01

    Our aim is to investigate the cytobiological effects of autologous platelet-rich fibrin (PRF) on dental pulp stem cells (DPSCs) and to explore the ectopic and orthotopic possibilities of dental pulp revascularization and pulp-dentin complex regeneration along the root canal cavities of the tooth by using a novel tissue-engineered transplant composed of cell-sheet fragments of DPSCs and PRF granules. Canine DPSCs were isolated and characterized by assaying their colony-forming ability and by determining their cell surface markers and osteogenic/adipogenic differentiation potential. The biological effects of autologous PRF on DPSCs, including cell proliferation, alkaline phosphatase (ALP) activity and odonto-/osteogenic gene expression, were then investigated and quantified. A novel transplant consisting of cell-sheet fragments of DPSCs and PRF granules was adopted to regenerate pulp-dentin-like tissues in the root canal, both subcutaneously in nude mice and in the roots of canines. PRF promoted the proliferation of DPSCs in a dose- and time-dependent manner and induced the differentiation of DPSCs to odonto-/osteoblastic fates by increasing the expression of the Alp, Dspp, Dmp1 and Bsp genes. Transplantation of the DPSC/PRF construct led both to a favorable regeneration of homogeneous and compact pulp-like tissues with abundantly distributed blood capillaries and to the deposition of regenerated dentin along the intracanal walls at 8 weeks post-operation. Thus, the application of DPSC/PRF tissue constructs might serve as a potential therapy in regenerative endodontics for pulp revitalization or revascularization.

  4. Growth of hydroxyapatite on physiologically clotted fibrin capped gold nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Sastry, T P; Sundaraseelan, J; Swarnalatha, K; Sobhana, S S Liji; Makheswari, M Uma; Sekar, S; Mandal, A B [Central Leather Research Institute, Chennai-600020, Tamilnadu (India)], E-mail: sastrytp@hotmail.com, E-mail: abmandal@hotmail.com

    2008-06-18

    The growth of hydroxyapatite (HAp) on physiologically clotted fibrin (PCF)-gold nanoparticles is presented for the first time by employing a wet precipitation method. Fourier transform infrared (FTIR) spectroscopy confirmed the characteristic functionalities of PCF and HAp in the PCF-Au-HAp nanocomposite. Scanning electron microscopy (SEM) images have shown cuboidal nanostructures having a size in the range of 70-300 nm of HAp, whereas 2-50 nm sized particles were visualized in high-resolution transmission electron microscopy (TEM). Energy-dispersive x-ray (EDX) and x-ray diffraction (XRD) studies have confirmed the presence of HAp. These results show that gold nanoparticles with PCF acted as a matrix for the growth of HAp, and that PCF-Au-HAp nanocomposite is expected to have better osteoinductive properties.

  5. Fibrin powder turbidity measurement for rapid assessment of antiplasmins.

    Science.gov (United States)

    Exner, T; Rickard, K A; Kronenberg, H

    1975-11-01

    Fibrinolytic activity was determined from the rate of disappearance of turbidity in a suspension of heat-treated fibrin powder. Using this method for estimating residual fibrinolytic activity in mixtures of serum and plasmin, antiplasmin behaviors of specimens from patients with various clinical disorders were determined after long and short preincubation times. Slow-acting antiplasmins were found to be increased in a variety of conditions among these patients, while immediate acting antiplasmins were generally decreased, compared with those in specimens from a large pool of normal, healthy vounteers. Normal women taking oral contraceptives had consitently high levels of slow antiplasmins. Tests in vitro showed that the antifibrinolytic agents epsilon-aminocaproic acid, Trasylol and soybean trypsin inhibitor act only as fast antiplasmins.

  6. Platelet-rich-fibrin: A novel root coverage approach

    Directory of Open Access Journals (Sweden)

    Anilkumar K

    2009-01-01

    Full Text Available Treatment of gingival recession has become an important therapeutic issue due to increasing cosmetic demand. Multiple surgical procedures have been developed to obtain predictable esthetic root coverage. More specifically, after periodontal regenerative surgery, the aim is to achieve complete wound healing and regeneration of the periodontal unit. A recent innovation in dentistry is the preparation and use of platelet-rich plasma (PRP, a concentrated suspension of the growth factors, found in platelets. These growth factors are involved in wound healing and postulated as promoters of tissue regeneration. This paper reports the use of PRF membrane for root coverage on the labial surfaces of the mandibular anterior teeth. This was accomplished using laterally displaced flap technique with platelet rich fibrin (PRF membrane at the recipient site.

  7. Autologous fat grafting: use of closed syringe microcannula system for enhanced autologous structural grafting

    Directory of Open Access Journals (Sweden)

    Alexander RW

    2013-04-01

    Full Text Available Robert W Alexander,1 David Harrell2 1Department of Surgery, School of Medicine and Dentistry, University of Washington, Seattle, WA, USA; 2Harvest-Terumo Inc, Plymouth, MA, USA Objectives: Provide background for use of acquiring autologous adipose tissue as a tissue graft and source of adult progenitor cells for use in cosmetic plastic surgery. Discuss the background and mechanisms of action of closed syringe vacuum lipoaspiration, with emphasis on accessing adipose-derived mesenchymal/stromal cells and the stromal vascular fraction (SVF for use in aesthetic, structural reconstruction and regenerative applications. Explain a proven protocol for acquiring high-quality autologous fat grafts (AFG with use of disposable, microcannula systems. Design: Explain the components and advantage of use of the patented super luer-lock and microcannulas system for use with the closed-syringe system. A sequential explanation of equipment selection for minimally traumatic lipoaspiration in small volumes is presented, including use of blunt injection cannulas to reduce risk of embolism. Results: Thousands of AFG have proven safe and efficacious for lipoaspiration techniques for large and small structural fat grafting procedures. The importance and advantages of gentle harvesting of the adipose tissue complex has become very clear in the past 5 years. The closed-syringe system offers a minimally invasive, gentle system with which to mobilize subdermal fat tissues in a suspension form. Resulting total nuclear counting of undifferentiated cells of the adipose-derived -SVF suggests that the yield achieved is better than use of always-on, constant mechanical pump applied vacuum systems. Conclusion: Use of a closed-syringe lipoaspiration system featuring disposable microcannulas offers a safe and effective means of harvesting small volumes of nonmanipulated adipose tissues and its accompanying progenitor cells within the SVF. Closed syringes and microcannulas are

  8. A recently developed bifacial platelet-rich fibrin matrix

    Directory of Open Access Journals (Sweden)

    E Lucarelli

    2010-07-01

    Full Text Available Platelet-rich plasma (PRP is used clinically in liquid or gel form to promote tissue repair. Because of the poor mechanical properties, conventional PRP is often difficult to handle when used in clinical settings and requires secure implantation in a specific site, otherwise when released growth factors could be washed out during an operation. In this study, we analyzed the end product of a recently developed commercially available system (FIBRINET®, which is a dense pliable, platelet-rich fibrin matrix (PRFM. We characterized the mechanical properties of PRFM and tested whether PRFM releases growth factors and whether released factors induce the proliferation of mesenchymal stem cells (MSC. Mechanical properties as well as platelet distribution were evaluated in PRFM. PRFM demonstrated robust mechanical properties, with a tear elastic modulus of 937.3 + 314.6 kPa, stress at a break of 1476.0 + 526.3 kPa, and an elongation at break of 146.3 + 33.8 %. PRFM maintained its mechanical properties throughout the testing process. Microscopic observations showed that the platelets were localized on one side of the matrix. Elevated levels of PDGF-AA, PDGF-AB, EGF, VEGF, bFGF and TGF-β1 were measured in the day 1-conditioned media (CM of PRFM and growth factor levels decreased thereafter. BMP2 and BMP7 were not detectable. MSC culture media supplemented with 20% PRFM-CM stimulated MSC cell proliferation; at 24 and 48 hours the induction of the proliferation was significantly greater than the induction obtained with media supplemented with 20% foetal bovine serum. The present study shows that the production of a dense, physically robust PRFM made through high-speed centrifugation of intact platelets and fibrin in the absence of exogenous thrombin yields a potential tool for accelerating tissue repair.

  9. A recently developed bifacial platelet-rich fibrin matrix.

    Science.gov (United States)

    Lucarelli, E; Beretta, R; Dozza, B; Tazzari, P L; O'Connel, S M; Ricci, F; Pierini, M; Squarzoni, S; Pagliaro, P P; Oprita, E I; Donati, D

    2010-07-01

    Platelet-rich plasma (PRP) is used clinically in liquid or gel form to promote tissue repair. Because of the poor mechanical properties, conventional PRP is often difficult to handle when used in clinical settings and requires secure implantation in a specific site, otherwise when released growth factors could be washed out during an operation. In this study, we analyzed the end product of a recently developed commercially available system (FIBRINET), which is a dense pliable, platelet-rich fibrin matrix (PRFM). We characterized the mechanical properties of PRFM and tested whether PRFM releases growth factors and whether released factors induce the proliferation of mesenchymal stem cells (MSC). Mechanical properties as well as platelet distribution were evaluated in PRFM. PRFM demonstrated robust mechanical properties, with a tear elastic modulus of 937.3 +/- 314.6 kPa, stress at a break of 1476.0 +/- 526.3 kPa, and an elongation at break of 146.3 +/- 33.8 %. PRFM maintained its mechanical properties throughout the testing process. Microscopic observations showed that the platelets were localized on one side of the matrix. Elevated levels of PDGF-AA, PDGF-AB, EGF, VEGF, bFGF and TGF-beta1 were measured in the day 1-conditioned media (CM) of PRFM and growth factor levels decreased thereafter. BMP2 and BMP7 were not detectable. MSC culture media supplemented with 20% PRFM-CM stimulated MSC cell proliferation; at 24 and 48 hours the induction of the proliferation was significantly greater than the induction obtained with media supplemented with 20% foetal bovine serum. The present study shows that the production of a dense, physically robust PRFM made through high-speed centrifugation of intact platelets and fibrin in the absence of exogenous thrombin yields a potential tool for accelerating tissue repair.

  10. Lower reoperation rates with the use of fibrin sealant versus tacks for mesh fixation

    DEFF Research Database (Denmark)

    Helvind, Neel Maria; Andresen, Kristoffer; Rosenberg, Jacob

    2013-01-01

    BACKGROUND: Groin hernia repair may be associated with long-term complications such as chronic pain, believed to result from damage to regional nerves by tissue penetrating mesh fixation. Studies have shown that mesh fixation with fibrin sealant reduces the risk of these long-term complications......, but data on recurrence and reoperation rates after the use of fibrin sealant compared with tacks are not available. This study aimed to determine whether fibrin sealant is a safe and feasible alternative to tacks with regard to reoperation rates after laparoscopic groin hernia repair. METHODS: The current...... study compared reoperation rates after laparoscopic groin hernia repair between fibrin sealant and tacks used for mesh fixation. The study used data collected prospectively from The National Danish Hernia Database and analyzed 8,314 laparoscopic groin hernia repairs for reoperation rates. Mesh fixation...

  11. 生物蛋白胶在急性胆囊炎LC术中的应用体会%Application Experience of Organism Albumin Glue in Acute Cholecystitis in Laparoscopic Cholecystectomy

    Institute of Scientific and Technical Information of China (English)

    张小红; 贾晓巍; 黄秋林; 杨林

    2002-01-01

    目的:探讨生物蛋白胶(biomedical fibrin glue,BFG)在急性胆囊炎LC术中应用价值.方法:随机选取生物蛋白胶治疗组96例患者和87例对照组患者.通过比较术中止血时间、术后腹腔引流量及引流时间、胆瘘发生情况、术后右上腹不适情况、住院时间等6项指标来说明其有效性.结果:除胆瘘发生情况,治疗组与对照组无显著差异外,其余5项均有显著性差异.结论:在急性胆囊炎LC术中应用生物蛋白胶,使手术止血时间缩短,防止创面出血和渗出,预防组织粘连,减少了患者痛苦并缩短了住院时间,减少了住院费用.

  12. 医用生物蛋白胶在腹腔镜胆总管探查术中的应用%Application of Medical Albumin Glue in Laparoscope Choledochus Exploration

    Institute of Scientific and Technical Information of China (English)

    张小红; 黄秋林; 贾晓巍; 杨林

    2002-01-01

    目的:评价医用生物蛋白胶(biomedical fibrin glue,BFG)在腹腔镜胆总管探查术(LCDE)中的应用价值. 方法:观察102例腹腔镜下胆总管探查术中应用生物蛋白胶患者术后腹腔引流量及引流管留置时间、胆漏发生率、术后1周胆囊窝积液量、术后1月右上腹不适或腹痛发生率,并与对照组进行比较.结果:治疗组腹腔引流量、引流管留置时间、住院时间明显少于对照组(P<0.01或0.05),胆汁漏发生率、术后右上腹不适或腹痛发生率亦明显低于对照组(P<0.05).结论:医用生物蛋白胶在腹腔镜下胆总管探查术中具有减少术后创面渗出和胆漏发生、缩短住院时间、减少术后痛苦、促进病情恢复等优点.

  13. High-Pressure Fibrin Sealant Foam: An Effective Hemostatic Agent for Treating Severe Parenchymal Hemorrhage

    Science.gov (United States)

    2008-01-01

    compression or vascular control. Materials and methods. FSF with high fibrinogen concentration (20 mg/mL) and low thrombin activity (5 U/mL) was prepared and...fibrin sealant foam (FSF) into a body cavity via trocar would spread throughout the cavity, bind to damaged tissue, and stop bleeding from inaccessible...liver hemorrhage model in anticoagulated rabbits. We then compared pressurized FSF with liq- uid and a powdered form of fibrin sealant. MATERIALS AND

  14. Does Fibrin Sealant Reduce Seroma after Immediate Breast Reconstruction Utilizing a Latissimus Dorsi Myocutaneous Flap?

    Directory of Open Access Journals (Sweden)

    Han Gyu Cha

    2012-09-01

    Full Text Available Background The most common complication of latissimus dorsi myocutaneous flap in breastreconstruction is seroma formation in the back. Many clinical studies have shown that fibrinsealant reduces seroma formation. We investigated any statistically significant differences inpostoperative drainage and seroma formation when utilizing the fibrin sealant on the site ofthe latissimus dorsi myocutaneous flap harvested for immediate breast reconstruction afterskin-sparing partial mastectomy.Methods A total of 46 patients underwent immediate breast reconstruction utilizing alatissimus dorsi myocutaneous island flap. Of those, 23 patients underwent the procedurewithout fibrin sealant and the other 23 were administered the fibrin sealant. All flaps wereelevated with manual dissection by the same surgeon and were analyzed to evaluate thepotential benefits of the fibrin sealant. The correlation analysis and Mann-Whitney U testwere used for analyzing the drainage volume according to age, weight of the breast specimen,and body mass index.Results Although not statistically significant, the cumulative drainage fluid volume was higherin the control group until postoperative day 2 (530.1 mL compared to 502.3 mL, but thefibrin sealant group showed more drainage beginning on postoperative day 3. The donor sitecomparisons showed the fibrin sealant group had more drainage beginning on postoperativeday 3 and the drain was removed 1 day earlier in the control group.Conclusions The use of fibrin sealant resulted in no reduction of seroma formation. Becausethe benefits of the fibrin sealant are not clear, the use of fibrin sealant must be fully discussedwith patients before its use as a part of informed consent.

  15. [APPLICATION OF FISTULA PLUG WITH THE FIBRIN ADHESIVE IN TREATMENT OF RECTAL FISTULAS].

    Science.gov (United States)

    Aydinova, P R; Aliyev, E A

    2015-05-01

    Results of surgical treatment of 21 patients, suffering high transsphincteric and extrasphincteric rectal fistulas, were studied. In patients of Group I the fistula passage was closed, using fistula plug obturator; and in patients of Group II--by the same, but preprocessed by fibrin adhesive. The fistula aperture germeticity, prophylaxis of rude cicatrices development in operative wound zone, promotion of better fixation of bioplastic material were guaranteed, using fistula plug obturator with preprocessing, using fibrin adhesive.

  16. Interpenetrating Fibrin-Alginate Matrices for in vitro Ovarian Follicle Development

    OpenAIRE

    Shikanov, Ariella; Xu, Min; Woodruff, Teresa K.; Shea, Lonnie D.

    2009-01-01

    In this report, we investigate the fibrin-alginate inter penetrating network (FA-IPN) to provide dynamic cell-responsive mechanical properties, which we apply to the in vitro growth of ovarian follicles. The mechanical properties and polymerization rate of the gels were investigated by rheology, and the fiber structure was imaged by electron microscopy. Using a mouse model, two-layered secondary follicles were encapsulated in FA-IPNs, and growth, morphology, hormone production, fibrin degrada...

  17. A neoantigenic determinant in the D-dimer fragment of fibrin

    NARCIS (Netherlands)

    Lugovskoy, E.V.; Kolesnikova, I.N.; Gritsenko, P.G.; Zolotareva, E.N.; Gaffney, P.; Nieuwenhuizen, W.; Komisarenko, S.V

    2002-01-01

    Monoclonal antibody (mAb) III-3b binds D-dimer with Kd = 1.4 × 10-10 M without cross-reaction with fibrin(ogen). The epitope for this mAb is in Bβ134-190, presumably in Bβ155-160. The latter site is buried in the coiled coil structure of fibrin(ogen) but it is exposed as a neoantigenic determinant i

  18. An Antithrombin-Heparin Complex Increases the Anticoagulant Activity of Fibrin Clots

    Directory of Open Access Journals (Sweden)

    Lesley J. Smith

    2008-01-01

    Full Text Available Clotting blood contains fibrin-bound thrombin, which is a major source of procoagulant activity leading to clot extension and further activation of coagulation. When bound to fibrin, thrombin is protected from inhibition by antithrombin (AT + heparin but is neutralized when AT and heparin are covalently linked (ATH. Here, we report the surprising observation that, rather than yielding an inert complex, thrombin-ATH formation converts clots into anticoagulant surfaces that effectively catalyze inhibition of thrombin in the surrounding environment.

  19. Role of platelet-rich-fibrin in enhancing palatal wound healing after free graft

    OpenAIRE

    2012-01-01

    Platelet-rich fibrin has long been used as a wound healing therapy in skin wounds and recently evidence has suggested its usage in oral cavity for different treatment procedures. This article proposes an overview of use of platelet-rich fibrin in management of complicated oral wounds. Excessive hemorrhage of the donor area, necrosis of epithelium, and morbidity associated with donor site have been described as the possible complications after harvesting subepithelial connective tissue graft, ...

  20. Fibrin Gel as an Injectable Biodegradable Scaffold and Cell Carrier for Tissue Engineering

    Directory of Open Access Journals (Sweden)

    Yuting Li

    2015-01-01

    Full Text Available Due to the increasing needs for organ transplantation and a universal shortage of donated tissues, tissue engineering emerges as a useful approach to engineer functional tissues. Although different synthetic materials have been used to fabricate tissue engineering scaffolds, they have many limitations such as the biocompatibility concerns, the inability to support cell attachment, and undesirable degradation rate. Fibrin gel, a biopolymeric material, provides numerous advantages over synthetic materials in functioning as a tissue engineering scaffold and a cell carrier. Fibrin gel exhibits excellent biocompatibility, promotes cell attachment, and can degrade in a controllable manner. Additionally, fibrin gel mimics the natural blood-clotting process and self-assembles into a polymer network. The ability for fibrin to cure in situ has been exploited to develop injectable scaffolds for the repair of damaged cardiac and cartilage tissues. Additionally, fibrin gel has been utilized as a cell carrier to protect cells from the forces during the application and cell delivery processes while enhancing the cell viability and tissue regeneration. Here, we review the recent advancement in developing fibrin-based biomaterials for the development of injectable tissue engineering scaffold and cell carriers.

  1. Fibrin Networks Support Recurring Mechanical Loads by Adapting their Structure across Multiple Scales.

    Science.gov (United States)

    Kurniawan, Nicholas A; Vos, Bart E; Biebricher, Andreas; Wuite, Gijs J L; Peterman, Erwin J G; Koenderink, Gijsje H

    2016-09-06

    Tissues and cells sustain recurring mechanical loads that span a wide range of loading amplitudes and timescales as a consequence of exposure to blood flow, muscle activity, and external impact. Both tissues and cells derive their mechanical strength from fibrous protein scaffolds, which typically have a complex hierarchical structure. In this study, we focus on a prototypical hierarchical biomaterial, fibrin, which is one of the most resilient naturally occurring biopolymers and forms the structural scaffold of blood clots. We show how fibrous networks composed of fibrin utilize irreversible changes in their hierarchical structure at different scales to maintain reversible stress stiffening up to large strains. To trace the origin of this paradoxical resilience, we systematically tuned the microstructural parameters of fibrin and used a combination of optical tweezers and fluorescence microscopy to measure the interactions of single fibrin fibers for the first time, to our knowledge. We demonstrate that fibrin networks adapt to moderate strains by remodeling at the network scale through the spontaneous formation of new bonds between fibers, whereas they adapt to high strains by plastic remodeling of the fibers themselves. This multiscale adaptation mechanism endows fibrin gels with the remarkable ability to sustain recurring loads due to shear flows and wound stretching. Our findings therefore reveal a microscopic mechanism by which tissues and cells can balance elastic nonlinearity and plasticity, and thus can provide microstructural insights into cell-driven remodeling of tissues.

  2. Fibrin clot properties and haemostatic function in men and women with type 1 diabetes.

    Science.gov (United States)

    Tehrani, Sara; Jörneskog, Gun; Ågren, Anna; Lins, Per-Eric; Wallén, Håkan; Antovic, Aleksandra

    2015-02-01

    The increased risk of vascular complications in type 1 diabetes may in part be explained by changes in haemostatic function. In the present study, we investigated the fibrin clot properties in patients with type 1 diabetes in relation to sex and microvascular complications. The study included 236 patients (107 women) aged between 20-70 years and without any history of cardiovascular disease. Fibrin clot properties, assessed by determination of the permeability coefficient (Ks) and turbidimetric clotting and lysis assays, did not differ between men and women. Compared with men, women had worse glycaemic control as well as higher levels of prothrombin fragment 1+2 and peak thrombin generation in vitro, indicating increased thrombin generation both in vivo and in vitro. Subgroup analyses of patients younger than 30 years revealed less permeable fibrin clots and prolonged lysis time in females compared with age-matched men. Patients with microvascular complications had higher fibrinogen concentrations and denser and less permeable fibrin clots. Thus, we conclude that in vitro fibrin clot properties in patients with type 1 diabetes without cardiovascular disease are not different between the sexes, but associate with prevalence of microvascular complications. Tighter fibrin clot formation in younger women, as suggested by our results, may affect their future cardiovascular risk and should be investigated in a larger population.

  3. Taurolidine-Fibrin-Sealant-Matrix using spray application for local treatment of brain tumors.

    Science.gov (United States)

    Stendel, Ruediger; Scheurer, Louis; Schlatterer, Kathrin; Gminski, Richard; Möhler, Hanns

    2004-01-01

    Malignant gliomas tend to recur in the vast majority of cases. Recurrent gliomas may arise from vital tumor cells present in this zone around the resection margin. It appears promising to combine tumor resection with local chemotherapy using an antineoplastic, but non-toxic agent. Taurolidine exerts a selective antineoplastic effect by induction of programmed cell death and has anti-angiogenic activity. Fibrin sealant is completely degradable and firmly adheres to brain tissue, suggesting that it would provide a suitable matrix for taurolidine delivery--a Taurolidine-Fibrin-Sealant-Matrix (TFM)--in the local treatment of brain tumors. The potential of local delivery of taurolidine out of a fibrin sealant matrix was investigated. Taurolidine could be suspended homogeneously in both the thrombin and the procoagulant protein components of the fibrin sealant. The fibrin sealant matrix was a suitable carrier for the suspension of taurolidine at a concentration that ensured the release of therapeutically effective amounts of the drug over a period of 2 weeks in vitro. The antineoplastic action of taurolidine was not affected by embedding in the fibrin sealant matrix. The described drug delivery system may be suitable for local taurolidine treatment of brain tumors following complete or partial resection or of tumors that are non-resectable because of their location.

  4. Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization

    Directory of Open Access Journals (Sweden)

    Jarel K. Gandhi

    2015-09-01

    Full Text Available Enhanced vascularization at sensor interfaces can improve long-term function. Fibrin, a natural polymer, has shown promise as a biomaterial for sensor coating due to its ability to sustain endothelial cell growth and promote local vascularization. However, the culture of cells, particularly endothelial cells (EC, within 3D scaffolds for more than a few days is challenging due to rapid loss of EC viability. In this manuscript, a robust method for developing fibrin microbead scaffolds for long-term culture of encapsulated ECs is described. Fibrin microbeads are formed using sodium alginate as a structural template. The size, swelling and structural properties of the microbeads were varied with needle gauge and composition and concentration of the pre-gel solution. Endothelial colony-forming cells (ECFCs were suspended in the fibrin beads and cultured within a perfusion bioreactor system. The perfusion bioreactor enhanced ECFCs viability and genome stability in fibrin beads relative to static culture. Perfusion bioreactors enable 3D culture of ECs within fibrin beads for potential application as a sensor coating.

  5. A Novel Platelet Concentrate: Titanium-Prepared Platelet-Rich Fibrin

    Directory of Open Access Journals (Sweden)

    Mustafa Tunalı

    2014-01-01

    Full Text Available We developed a new product called titanium-prepared platelet-rich fibrin (T-PRF. The T-PRF method is based on the hypothesis that titanium may be more effective in activating platelets than the silica activators used with glass tubes in Chouckroun’s leukocyte- and platelet-rich fibrin (L-PRF method. In this study, we aimed to define the structural characteristics of T-PRF and compare it with L-PRF. Blood samples were collected from 10 healthy male volunteers. The blood samples were drawn using a syringe. Nine milliliters was transferred to a dry glass tube, and 9 mL was transferred to a titanium tube. Half of each clot (i.e., the blood that was clotted using T-PRF or L-PRF was processed with a scanning electron microscope (SEM. The other half of each clot was processed for fluorescence microscopy analysis and light microscopy analysis. The T-PRF samples seemed to have a highly organized network with continuous integrity compared to the other L-PRF samples. Histomorphometric analysis showed that T-PRF fibrin network covers larger area than L-PRF fibrin network; also fibrin seemed thicker in the T-PRF samples. This is the first human study to define T-PRF as an autogenous leukocyte- and platelet-rich fibrin product. The platelet activation by titanium seems to offer some high characteristics to T-PRF.

  6. A novel platelet concentrate: titanium-prepared platelet-rich fibrin.

    Science.gov (United States)

    Tunalı, Mustafa; Özdemir, Hakan; Küçükodacı, Zafer; Akman, Serhan; Yaprak, Emre; Toker, Hülya; Fıratlı, Erhan

    2014-01-01

    We developed a new product called titanium-prepared platelet-rich fibrin (T-PRF). The T-PRF method is based on the hypothesis that titanium may be more effective in activating platelets than the silica activators used with glass tubes in Chouckroun's leukocyte- and platelet-rich fibrin (L-PRF) method. In this study, we aimed to define the structural characteristics of T-PRF and compare it with L-PRF. Blood samples were collected from 10 healthy male volunteers. The blood samples were drawn using a syringe. Nine milliliters was transferred to a dry glass tube, and 9 mL was transferred to a titanium tube. Half of each clot (i.e., the blood that was clotted using T-PRF or L-PRF) was processed with a scanning electron microscope (SEM). The other half of each clot was processed for fluorescence microscopy analysis and light microscopy analysis. The T-PRF samples seemed to have a highly organized network with continuous integrity compared to the other L-PRF samples. Histomorphometric analysis showed that T-PRF fibrin network covers larger area than L-PRF fibrin network; also fibrin seemed thicker in the T-PRF samples. This is the first human study to define T-PRF as an autogenous leukocyte- and platelet-rich fibrin product. The platelet activation by titanium seems to offer some high characteristics to T-PRF.

  7. Identification of quantitative trait loci for fibrin clot phenotypes: The EuroCLOT study

    Science.gov (United States)

    Williams, Frances MK; Carter, Angela M; Kato, Bernet; Falchi, Mario; Bathum, Lise; Surdulescu, Gabriela; Kyvik, Kirsten Ohm; Palotie, Aarno; Spector, Tim D; Grant, Peter J

    2012-01-01

    Objectives Fibrin makes up the structural basis of an occlusive arterial thrombus and variability in fibrin phenotype relates to cardiovascular risk. The aims of the current study from the EU consortium EuroCLOT were to 1) determine the heritability of fibrin phenotypes and 2) identify QTLs associated with fibrin phenotypes. Methods 447 dizygotic (DZ) and 460 monozygotic (MZ) pairs of healthy UK Caucasian female twins and 199 DZ twin pairs from Denmark were studied. D-dimer, an indicator of fibrin turnover, was measured by ELISA and measures of clot formation, morphology and lysis were determined by turbidimetric assays. Heritability estimates and genome-wide linkage analysis were performed. Results Estimates of heritability for d-dimer and turbidometric variables were in the range 17 - 46%, with highest levels for maximal absorbance which provides an estimate of clot density. Genome-wide linkage analysis revealed 6 significant regions with LOD>3 on 5 chromosomes (5, 6, 9, 16 and 17). Conclusions The results indicate a significant genetic contribution to variability in fibrin phenotypes and highlight regions in the human genome which warrant further investigation in relation to ischaemic cardiovascular disorders and their therapy. PMID:19150881

  8. Autologous Serum Skin Test versus Autologous Plasma Skin Test in Patients with Chronic Spontaneous Urticaria

    Directory of Open Access Journals (Sweden)

    Aysegul Alpay

    2013-01-01

    Full Text Available Previous studies indicate that 25–45% of chronic urticaria patients have an autoimmune etiology. Autologous serum skin test (ASST and autologous plasma skin test (APST are simple tests for diagnosing chronic autoimmune urticaria (CAU. However, there are still some questions about the specificity of these tests. This study consisted of 50 patients with chronic spontaneous urticaria (CSU and 50 sex- and age-matched healthy individuals aged 18 years, and older. A total of 31 (62% patients and 5 (10% control patients had positive ASST; 21 (42% patients and 3 (6% control patients had positive APST. Statistically significant differences were noted in ASST and APST positivity between the patient and control groups (ASST P<0.001; APST P<0.001. Thirteen (26% patients and 5 (10% control patients had antithyroglobulin antibodies or antithyroid peroxidase antibody positivity. No statistically significant differences were noted in thyroid autoantibodies between the patient and control groups (anti-TG P=0.317; anti-TPO P=0.269. We consider that the ASST and APST can both be used as in vivo tests for the assessment of autoimmunity in the etiology of CSU and that thyroid autoantibodies should be checked even when thyroid function tests reveal normal results in patients with CSU.

  9. Ultrastructural characteristics of fibrin clots from canine and feline platelet concentrates activated with calcium gluconate or calcium gluconate plus batroxobin

    OpenAIRE

    Silva, Raúl F; Carmona, Jorge U; Rezende, Cleuza MF

    2013-01-01

    Background The aim of this study was to use transmission electron microscopy to describe the ultrastructural characteristics of clots obtained from canine and feline platelet concentrates (PC) that had been activated with calcium gluconate (CG) or CG plus batroxobin (CGB). Platelets from fibrin clots were classified according their morphological changes. The area of the intercellular space (μm2), the area of the fibrin fibers (μm2), and the width of the fibrin fibers (μm) were determined for ...

  10. Preoperative embolization of nasopharyngeal angiofibromas: The role of direct percutaneous injection of cyanoacrylate glue in conjunction with particulate endovascular approach

    Directory of Open Access Journals (Sweden)

    Mohamed Abdel Hakim Osman Kasem

    2016-12-01

    Conclusion: The embolization of nasopharyngeal angiofibromas before surgery using percutaneous cyanoacrylate glue with endovascular particulate material proved to efficiently devascularize these tumours with lower blood loss during surgery and no major procedural complications.

  11. Method for Management of Perianal Fistula with New Device: Progressive Curettage of the Tract and Sealing with Platelet-Rich Fibrin

    Directory of Open Access Journals (Sweden)

    Perez Lara

    2016-08-01

    Full Text Available Surgical treatment of high perianal fistulas, which affect a significant proportion of the sphincter apparatus, is difficult and associated with considerable risk of impaired anal continence. The diversity of approaches proposed for the treatment of complex perianal fistulas reflects the fact that no method has yet been shown to be fully satisfactory. We believe the successful treatment of this condition is directly proportional to the amount of fibrous tissue that can be removed. We used a kit of small curettes, of different thicknesses and sizes, incorporating spicules that enable the physician to remove fibrous tissue from the fistula tract. The small size and varying thicknesses of the curettes enable them to mold to the curves of the fistula tract and to remove tissue by de-roofing from the shallowest to the deepest layers, thus excising the entire fibrous tract. The tract is then sealed using autologous fibrin, applied through a catheter, with the help of a monitor indicating the amount of product remaining at all times. Finally, the internal orifice is closed by simple suturing.

  12. Comparative clinical evaluation of coronally advanced flap with or without platelet rich fibrin membrane in the treatment of isolated gingival recession

    Directory of Open Access Journals (Sweden)

    Murugan Thamaraiselvan

    2015-01-01

    Full Text Available Aim: The aim of this study was to determine whether the addition of an autologous platelet rich fibrin (PRF membrane to a coronally advanced flap (CAF would improve the clinical outcome in terms of root coverage, in the treatment of isolated gingival recession. Materials and Methods: Systemically healthy 20 subjects each with single Miller′s class I or II buccal recession defect were randomly assigned to control (CAF or test (CAF + PRF group. Clinical outcome was determined by measuring the following clinical parameters such as recession depth (RD, recession width (RW, probing depth (PD, clinical attachment level (CAL, width of keratinized tissue (WKT, gingival thickness (GTH, plaque index (PI, and gingival index (GI at baseline, 3 rd , and 6 th month postsurgery. Results: The root coverage was 65.00 ΁ 44.47% in the control group and 74.16 ΁ 28.98% in the test group at 6 th month, with no statistically significant difference between them. Similarly, CAL, PD, and WKT between the groups were not statistically significant. Conversely, there was statistically significant increase in GTH in the test group. Conclusion: CAF is a predictable treatment for isolated Miller′s class I and II recession defects. The addition of PRF to CAF provided no added advantage in terms of root coverage except for an increase in GTH.

  13. Comparison of Achilles tendon repair techniques in a sheep model using a cross-linked acellular porcine dermal patch and platelet-rich plasma fibrin matrix for augmentation.

    Science.gov (United States)

    Sarrafian, Tiffany L; Wang, Hali; Hackett, Eileen S; Yao, Jian Q; Shih, Mei-Shu; Ramsay, Heather L; Turner, A Simon

    2010-01-01

    The primary goal of this study was to evaluate a cross-linked acellular porcine dermal patch (APD), as well as platelet-rich plasma fibrin matrix (PRPFM), for repair of acute Achilles tendon rupture in a sheep model. The 2 surgically transected tendon ends were reapproximated in groups 1 and 2, whereas a gap was left between the tendon ends in group 3. APD was used to reinforce the repair in group 2, and autologous PRPFM was used to fill the gap, which was also reinforced with APD, in group 3. All sheep were humanely euthanized at 24 weeks after the repair, and biomechanical and histological testing were performed. Tensile strength testing showed a statistically significant difference in elongation between the operated limb and the unoperated contralateral limb in groups 1 and 3, but not in group 2. All operated tendons appeared healed with no apparent fibrosis under light and polarized microscopy. In group 1, all surgical separation sites were identifiable, and healing occurred via increasing tendon thickness. In group 2, healing occurred with new tendon fibers across the separation, without increasing tendon thickness in 2 out of 6 animals. Group 3 showed complete bridging of the gap, with no change in tendon thickness in 2 out of 6 animals. In groups 2 and 3, peripheral integration of the APD to tendon fibers was observed. These findings support the use of APD, alone or with PRPFM, to augment Achilles tendon repair in a sheep model.

  14. [The use of platelet concentrates: platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in bone reconstruction prior to dental implant surgery].

    Science.gov (United States)

    Baeyens, W; Glineur, R; Evrard, L

    2010-01-01

    The autologous platelet concentrates--Platelet-Rich Plasma (PRP) and Platelet-Rich Fibrin (PRF)--are used in various medical fields, particularly in oral and maxillofacial surgery. These concentrates contain high levels of growth factors, including the 3 isomers of PDGF (platelet-derived growth factor), 2 of the numerous transforming growth factors (TGF-beta), the insulinlike growth factor (IGF), the epithelial growth factor (EGF) and the vascular endothelial growth factor (VEGF), which are the key elements in wound healing, particularly in bone regeneration. Platelet concentrates are easy to apply in clinical practice and offer potential benefits including rapid wound healing and bone regeneration, and can therefore be considered to be new therapeutic adjuvants. In dental implant surgery they are used in bone reconstruction prior or concomitant to implant procedures, and also for dental extraction socket preservation. Their use result in enhanced bone graft density and maturation. A literature review on the use of PRP/PRF in maxillofacial and dental implant surgery is proposed.

  15. PENGARUH JENIS DAN KONSENTRASI ASAM, TEMPERATUR DAN WAKTU EKSTRAKSI TERHADAP KARAKTERISTIK FISH GLUE DARI LIMBAH IKAN TENGGIRI

    Directory of Open Access Journals (Sweden)

    Tony Handoko

    2012-05-01

    Full Text Available THE EFFECTS OF TYPES AND ACID CONCENTRATIONS, TEMPERATURES AND EXTRACTION TIME ON THE FISH GLUE CHARACTERISTIC OBTAINED FROM MACKEREL FISH BONE WASTE. As a maritime nation, Indonesia produced fresh fish products up to 4,408,419 tons in 2005. Mackerel fish is one of them. Its bone waste has an economic value as a source for fish glue production. The purpose of this research was to determine the optimum conditions in fish glue processing. Preliminary research was done to determine the acid type (CH3COOH and HCl and its concentration (4%, 5%, and 6% in soaking process. While the main research was then done to determine the best temperature (45oC, 60oC, and 75oC and time of extraction (4 hrs, 5 hrs, 6 hrs. The fish glue products were analyzed their adhesion and physical characteristics, such as density, viscosity, pH, and water content. The results showed that weak acid (CH3COOH of 5% concentration is the best solution in soaking process and extraction in 4 hrs at 45oC has given the optimum condition for producing fish glue. The glue has a nice odor but its adhesion strength remains poor.      Abstrak   Sebagai negara maritim, Indonesia menghasilkan produk perikanan yang sangat besar mencapai 4.408.419 ton pada tahun 2005 dan terus bertambah. Salah satu produk perikanan yang terbesar adalah ikan tenggiri dengan limbah tulang yang mempunyai nilai ekonomis yang tinggi sebagai bahan baku fish glue. Penelitian ini bertujuan untuk menemukan kondisi proses yang dapat menghasilkan fish glue yang baik melalui dua tahap yaitu penelitian pendahuluan dan utama. Penelitian pendahuluan bertujuan mendapatkan jenis asam (CH3COOH dan HCl dan konsentrasi asam terbaik (4%, 5%, dan 6% pada  proses perendaman tulang ikan. Penelitian utama bertujuan  menentukan temperatur ekstraksi (45oC, 60oC, dan 75oC dan waktu ekstraksi (4 jam, 5 jam, 6 jam. Analisis fish glue yang dilakukan adalah uji kerekatan dan sifat fisik berupa densitas, viskositas, pH, dan kadar air

  16. Effectiveness of autologous transfusion system in primary total hip and knee arthroplasty.

    LENUS (Irish Health Repository)

    Schneider, Marco M

    2014-01-01

    Autologous transfusion has become a cost-efficient and useful option in the treatment of patients with high blood loss following major orthopaedic surgery. However, the effectiveness of autologous transfusion in total joint replacement remains controversial.

  17. Improving diagnosis of appendicitis. Early autologous leukocyte scanning.

    Science.gov (United States)

    DeLaney, A R; Raviola, C A; Weber, P N; McDonald, P T; Navarro, D A; Jasko, I

    1989-10-01

    A prospective nonrandomized study investigating the accuracy and utility of autologous leukocyte scanning in the diagnosis of apendicitis was performed. One hundred patients in whom the clinical diagnosis of appendicitis was uncertain underwent indium 111 oxyquinoline labelling of autologous leukocytes and underwent scanning 2 hours following reinjection. Of 32 patients with proved appendicitis, three scans revealed normal results (false-negative rate, 0.09). Of 68 patients without appendicitis, three scans had positive results (false-positive rate, 0.03; sensitivity, 0.91; specificity, 0.97; predictive value of positive scan, 0.94; predictive value of negative scan, 0.96; and overall accuracy, 0.95). Scan results altered clinical decisions in 19 patients. In 13 cases, the scan produced images consistent with diagnoses other than appendicitis, expediting appropriate management. Early-imaging111 In oxyquinoline autologous leukocyte scanning is a practical and highly accurate adjunct for diagnosing appendicitis.

  18. Therapeutic Potential of Autologous Stem Cell Transplantation for Cerebral Palsy

    Directory of Open Access Journals (Sweden)

    Chaitanya Purandare

    2012-01-01

    Full Text Available Background. Cerebral palsy (CP is a severe disabling disease with worldwide incidence being 2 to 3 per 1000 live births. CP was considered as a noncurable, nonreparative disorder, but stem cell therapy offers a potential treatment for CP. Objective. The present study evaluates the safety and efficacy of autologous bone-marrow-derived mononuclear cell (BMMNCs transplantation in CP patient. Material and Methods. In the present study, five infusions of autologous stem cells were injected intrathecally. Changes in neurological deficits and improvements in function were assessed using Gross Motor Function Classification System (GMFCS-E&R scale. Results. Significant motor, sensory, cognitive, and speech improvements were observed. Bowel and bladder control has been achieved. On the GMFCS-E&R level, the patient was promoted from grade III to I. Conclusion. In this study, we report that intrathecal infusion of autologous BMMNCs seems to be feasible, effective, and safe with encouraging functional outcome improvements in CP patient.

  19. AlGaInP-Si glue bonded high performance light emitting diodes

    Institute of Scientific and Technical Information of China (English)

    Chen Yi-Xin; Shen Guang-Di; Guo Wei-Ling; Gao Zhi-Yuan

    2011-01-01

    We propose a new method of using conductive glue to agglutinate GaAs based AlGaInP light emitting diodes (LEDs)onto silicon substrate,and the absorbing GaAs layer is subsequently removed by grinding and selective wet etching.It was found that AIGainP-Si glue agglutinated LEDs have larger saturation current and luminous intensity than the conventional LEDs working at the same injected current.The luminous intensity of the new device is as much as 1007.4 mcd at a saturation current of 125 mA without being encapsulated,while the conventional LEDs only have 266.2 mcd at a saturation current of 105 mA.The luminescence intensity is also found to increase by about 3.2% after working at 50 mA for 768 h.This means that the new structured LEDs have good reliability performance.

  20. Critical Speed of The Glass Glue Machine's Creep and Influence Factors Analysis

    Science.gov (United States)

    Yang, Jianxi; Huang, Jian; Wang, Liying; Shi, Jintai

    When automatic glass glue machine works, two questions of the machine starting vibrating and stick-slip motion are existing. These problems should be solved. According to these questions, a glue machine's model for studying stick-slip is established. Based on the dynamics system describing of the model, mathematical expression is presented. The creep critical speed expression is constructed referring to existing research achievement and a new conclusion is found. The influencing factors of stiffness, dampness, mass, velocity, difference of static and kinetic coefficient of friction are analyzed through Matlab simulation. Research shows that reasonable choice of influence parameters can improve the creep phenomenon. These all supply the theory evidence for improving the machine's motion stability.

  1. Development of Silicon Photomultipliers and their Applications to GlueX Calorimetry

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Elton S. [Thomas Jefferson National Accelerator Facility (TJNAF), Newport News, VA (United States)

    2016-07-01

    The GlueX experiment is a photoproduction experiment in Hall D at Jefferson Lab that is being commissioned for use with the new 12 GeV accelerator. The purpose of the experiment is to search for Hybrid mesons, which are mesons with quark and gluon degrees of freedom. The barrel calorimeter of GlueX is instrumented with 4000 large-area (1.2 x1.2 cm2) silicon photomultipliers (SiPMs). These photon sensors have properties similar to vacuum photomultipliers, but are unaffected by high magnetic fields. In our experiment they operate in magnetic fields exceeding 1T. After extensive tests with a variety of sensors, we chose the S12045(X) custom SiPM arrays manufactured by Hamamatsu Corporation, also known as multi-pixel photon counters (MPPCs). We will give an overview of this new technology as well as the experience gained during two commissioning periods with beam.

  2. Biological Glue Application in Repair of Atrioventricular Groove Rupture: A Case Report

    Directory of Open Access Journals (Sweden)

    Ahmet Baris-Durukan

    2015-10-01

    Full Text Available Atrioventricular groove rupture is a rare, albeit mortal, complication following mitral valve surgery. Avoidance is the best strategy but it cannot fully prevent the occurrence of this complication. Several repair techniques have been described with varying success rates; however, the rarity of the complication precludes consensus about the safest technique.Here we report two cases of posterior atrioventricular groove rupture. Both cases were diagnosed immediately after the cessation of cardiopulmonary bypass. Repair was performed successfully with a technique involving the use of biological glue. The postoperative course was uneventful for both of them. Both cases are well with normally functioning mitral prostheses; one with a follow-up time of 5.5 years and the other 10 months. We believe that the glue provides additional hemostasis and support to the repaired area.

  3. Development of silicon photomultipliers and their applications to GlueX calorimetry

    Science.gov (United States)

    Smith, Elton S.

    2016-07-01

    The GlueX experiment is a photoproduction experiment in Hall D at Jefferson Lab that is being commissioned for use with the new 12 GeV accelerator. The purpose of the experiment is to search for Hybrid mesons, which are mesons with quark and gluon degrees of freedom. The barrel calorimeter of GlueX is instrumented with 4000 large-area (1.2 × 1.2 cm2) silicon photomultipliers (SiPMs). These photon sensors have properties similar to vacuum photomultipliers, but are unaffected by high magnetic fields. In our experiment they operate in magnetic fields exceeding 1T. After extensive tests with a variety of sensors, we chose the S12045(X) custom SiPM arrays manufactured by Hamamatsu Corporation, also known as multi-pixel photon counters (MPPCs). We will give an overview of this new technology as well as the experience gained during two commissioning periods with beam.

  4. Study of nano imprinting using soft lithography on Krafty glue and PVDF polymer thin films

    Energy Technology Data Exchange (ETDEWEB)

    Sankar, M. S. Ravi, E-mail: rameshg.phy@pondiuni.edu; Gangineni, Ramesh Babu, E-mail: rameshg.phy@pondiuni.edu [Department of Physics, Pondicherry University, R. V. Nagar, Kalapet, Puducherry - 605014 (India)

    2014-04-24

    The present work reveals soft lithography strategy based on self assembly and replica molding for carrying out micro and nanofabrication. It provides a convenient, effective and very low cost method for the formation and manufacturing of micro and nano structures. Al-layer of compact disc (sony CD-R) used as a stamp with patterned relief structures to generate patterns and structures with pattern size of 100nm height, 1.7 μm wide. In literature, PDMS (Polydimethylsiloxane) solution is widely used to get negative copy of the Al-layer. In this work, we have used inexpensive white glue (Polyvinylacetate + water), 15gm (□5) and PVDF (Polyvinylidene difluoride) spin coated films and successfully transferred the nano patterns of Al layer on to white glue and PVDF films.

  5. Genipin-crosslinked fibrin hydrogels as a potential adhesive to augment intervertebral disc annulus repair

    Directory of Open Access Journals (Sweden)

    RM Schek

    2011-04-01

    Full Text Available Treatment of damaged intervertebral discs is a significant clinical problem and, despite advances in the repair and replacement of the nucleus pulposus, there are few effective strategies to restore defects in the annulus fibrosus. An annular repair material should meet three specifications: have a modulus similar to the native annulus tissue, support the growth of disc cells, and maintain adhesion to tissue under physiological strain levels. We hypothesized that a genipin crosslinked fibrin gel could meet these requirements. Our mechanical results showed that genipin crosslinked fibrin gels could be created with a modulus in the range of native annular tissue. We also demonstrated that this material is compatible with the in vitro growth of human disc cells, when genipin:fibrin ratios were 0.25:1 or less, although cell proliferation was slower and cell morphology more rounded than for fibrin alone. Finally, lap tests were performed to evaluate adhesion between fibrin gels and pieces of annular tissue. Specimens created without genipin had poor handling properties and readily delaminated, while genipin crosslinked fibrin gels remained adhered to the tissue pieces at strains exceeding physiological levels and failed at 15-30%. This study demonstrated that genipin crosslinked fibrin gels show promise as a gap-filling adhesive biomaterial with tunable material properties, yet the slow cell proliferation suggests this biomaterial may be best suited as a sealant for small annulus fibrosus defects or as an adhesive to augment large annulus repairs. Future studies will evaluate degradation rate, fatigue behaviors, and long-term biocompatibility.

  6. A safe and efficient method to retrieve mesenchymal stem cells from three-dimensional fibrin gels.

    Science.gov (United States)

    Carrion, Bita; Janson, Isaac A; Kong, Yen P; Putnam, Andrew J

    2014-03-01

    Mesenchymal stem cells (MSCs) display multipotent characteristics that make them ideal for potential therapeutic applications. MSCs are typically cultured as monolayers on tissue culture plastic, but there is increasing evidence suggesting that they may lose their multipotency over time in vitro and eventually cease to retain any resemblance to in vivo resident MSCs. Three-dimensional (3D) culture systems that more closely recapitulate the physiological environment of MSCs and other cell types are increasingly explored for their capacity to support and maintain the cell phenotypes. In much of our own work, we have utilized fibrin, a natural protein-based material that serves as the provisional extracellular matrix during wound healing. Fibrin has proven to be useful in numerous tissue engineering applications and has been used clinically as a hemostatic material. Its rapid self-assembly driven by thrombin-mediated alteration of fibrinogen makes fibrin an attractive 3D substrate, in which cells can adhere, spread, proliferate, and undergo complex morphogenetic programs. However, there is a significant need for simple cost-effective methods to safely retrieve cells encapsulated within fibrin hydrogels to perform additional analyses or use the cells for therapy. Here, we present a safe and efficient protocol for the isolation of MSCs from 3D fibrin gels. The key ingredient of our successful extraction method is nattokinase, a serine protease of the subtilisin family that has a strong fibrinolytic activity. Our data show that MSCs recovered from 3D fibrin gels using nattokinase are not only viable but also retain their proliferative and multilineage potentials. Demonstrated for MSCs, this method can be readily adapted to retrieve any other cell type from 3D fibrin gel constructs for various applications, including expansion, bioassays, and in vivo implantation.

  7. Inhibition of fibrin polymerization by synthetic peptides corresponding to Aalpha195-205 and gamma69-77 sites of fibrin molecule

    NARCIS (Netherlands)

    Pozniak, T.A.; Urvant, L.P.; Gritsenko, P.; Chernishov, V.I.; Pydiura, N.A.; Lugovskoi, E.V.; Komisarenko, S.V.

    2014-01-01

    Using the idea of "proline brackets" we have found four sites in fibrin amino acid sequence, and appropriate peptides were synthesized: gamma69NPDESSKPN77, Bbeta228QPDSSVKPY236, Bbeta455RPFFPQ460 and Aalpha195LPSRDRQHLPL205. Turbidity and electron-microscopy analyses have demonstrated that synthetic

  8. Glue-sniffing as a cause of a positive radio-isotope brain scan

    Energy Technology Data Exchange (ETDEWEB)

    Lamont, C.M.; Adams, F.G.

    1982-08-01

    Convulsions are a known complication of the acute intoxicant effects of solvent abuse. A radio-isotope brain scan done 9 months following status epilepticus secondary to toluene inhalation, in a previously normal school-boy, demonstrated several wedge-shaped areas of increased uptake, in both cerebral hemispheres, consistent with infarcts. It is worth remembering that a positive brain scan in a young person, with recent onset of epilepsy, may be due to glue-sniffing.

  9. GLUE Based Uncertainty Estimation of Urban Drainage Modeling Using Weather Radar Precipitation Estimates

    DEFF Research Database (Denmark)

    Nielsen, Jesper Ellerbæk; Thorndahl, Søren Liedtke; Rasmussen, Michael R.

    2011-01-01

    the uncertainty of the weather radar rainfall input. The main findings of this work, is that the input uncertainty propagate through the urban drainage model with significant effects on the model result. The GLUE methodology is in general a usable way to explore this uncertainty although; the exact width...... of the prediction bands can be questioned, due to the subjective nature of the method. Moreover, the method also gives very useful information about the model and parameter behaviour....

  10. Autologous adventitial overlay method reinforces anastomoses in aortic surgery.

    Science.gov (United States)

    Minato, Naoki; Okada, Takayuki; Sumida, Tomohiko; Watanabe, Kenichi; Maruyama, Takahiro; Kusunose, Takashi

    2014-05-01

    In this study, we present an inexpensive and effective method for providing a secure and hemostatic anastomosis using autologous adventitia obtained from a dissected or aneurysmal wall. The resected aortic wall is separated between the adventitia and media, and a soft, 2 × 10-cm adventitial strip is overlaid to cover the anastomotic margin. A graft is sutured to the aortic stump. This autologous adventitial overlay method can inexpensively and strongly reinforce the anastomosis during aortic surgery for dissection or aneurysm and will contribute to anastomotic hemostasis and long-term stability.

  11. Breast Imaging after Breast Augmentation with Autologous Tissues

    Energy Technology Data Exchange (ETDEWEB)

    Hwang, Kyu Won; Seo, Bo Kyung; Shim, Eddeum; Song, Sung Eun; Cho, Kyu Ran [Dept. of Radiology, Korea University Anam Hospital, Seoul (Korea, Republic of); Yoon, Eul Sik [Korea University Ansan Hospital, Ansan (Korea, Republic of); Woo, Ok Hee [Dept. of Radiology, Korea University Guro Hospital, Seoul (Korea, Republic of)

    2012-06-15

    The use of autologous tissue transfer for breast augmentation is an alternative to using foreign implant materials. The benefits of this method are the removal of unwanted fat from other body parts, no risk of implant rupture, and the same feel as real breast tissue. However, sometimes there is a dilemma about whether or not to biopsy for calcifications or masses detected after the procedure is completed. The purpose of this study is to illustrate the procedures of breast augmentation with autologous tissues, the imaging features of various complications, and the role of imaging in the diagnosis and management of complications and hidden breast diseases.

  12. Preoperative autologous plateletpheresis in patients undergoing open heart surgery.

    Directory of Open Access Journals (Sweden)

    Tomar Akhlesh

    2003-01-01

    Full Text Available Blood conservation is an important aspect of care provided to the patients undergoing cardiac operations with cardiopulmonary bypass (CPB. It is even more important in patients with anticipated prolonged CPB, redo cardiac surgery, patients having negative blood group and in patients undergoing emergency cardiac surgery. In prolonged CPB the blood is subjected to more destruction of important coagulation factors, in redo surgery the separation of adhesions leads to increased bleeding and difficulty in achieving the haemostasis and in patients with negative blood group and emergency operations, the availability of sufficient blood can be a problem. Harvesting the autologous platelet rich plasma (PRP can be a useful method of blood conservation in these patients. The above four categories of patients were prospectively studied, using either autologous whole blood donation or autologous platelet rich plasma (PRP harvest in the immediate pre-bypass period. Forty two patients were included in the study and randomly divided into two equal groups of 21 each, control group (Group I in which one unit of whole blood was withdrawn, and PRP group (Group II where autologous plateletpheresis was utilised. After reversal of heparin, autologous whole blood was transfused in the control group and autologous PRP was transfused in the PRP group. The chest tube drainage and the requirement of homologous blood and blood products were recorded. Average PRP harvest was 643.33 +/- 133.51 mL in PRP group and the mean whole blood donation was 333.75 +/- 79.58 mL in the control group. Demographic, preoperative and intra operative data showed no statistically significant differences between the two groups. The PRP group patients drained 26.44% less (p<0.001 and required 38.5% less homologous blood and blood products (p<0.05, in the postoperative period. Haemoglobin levels on day zero (day of operation and day three were statistically not different between the two groups. We

  13. Using CV-GLUE procedure in analysis of wetland model predictive uncertainty.

    Science.gov (United States)

    Huang, Chun-Wei; Lin, Yu-Pin; Chiang, Li-Chi; Wang, Yung-Chieh

    2014-07-01

    This study develops a procedure that is related to Generalized Likelihood Uncertainty Estimation (GLUE), called the CV-GLUE procedure, for assessing the predictive uncertainty that is associated with different model structures with varying degrees of complexity. The proposed procedure comprises model calibration, validation, and predictive uncertainty estimation in terms of a characteristic coefficient of variation (characteristic CV). The procedure first performed two-stage Monte-Carlo simulations to ensure predictive accuracy by obtaining behavior parameter sets, and then the estimation of CV-values of the model outcomes, which represent the predictive uncertainties for a model structure of interest with its associated behavior parameter sets. Three commonly used wetland models (the first-order K-C model, the plug flow with dispersion model, and the Wetland Water Quality Model; WWQM) were compared based on data that were collected from a free water surface constructed wetland with paddy cultivation in Taipei, Taiwan. The results show that the first-order K-C model, which is simpler than the other two models, has greater predictive uncertainty. This finding shows that predictive uncertainty does not necessarily increase with the complexity of the model structure because in this case, the more simplistic representation (first-order K-C model) of reality results in a higher uncertainty in the prediction made by the model. The CV-GLUE procedure is suggested to be a useful tool not only for designing constructed wetlands but also for other aspects of environmental management.

  14. The impact of UVB radiation on the glycoprotein glue of orb-weaving spider capture thread.

    Science.gov (United States)

    Stellwagen, Sarah D; Opell, Brent D; Clouse, Mary E

    2015-09-01

    Many spider orb-webs are exposed to sunlight and the potentially damaging effects of ultraviolet B (UVB) radiation. We examined the effect of UVB on the viscoelastic glycoprotein core of glue droplets deposited on the prey capture threads of these webs, hypothesizing that webs built by species that occupy sunny habitats are less susceptible to UVB damage than are webs built by species that prefer shaded forest habitats or by nocturnal species. Threads were tested shortly after being collected in the early morning and after being exposed to UVB energy equivalent to a day of summer sun and three times this amount. Droplets kept in a dark chamber allowed us to evaluate post-production changes. Droplet volume was unaffected by treatments, indicating that UVB did not damage the hygroscopic compounds in the aqueous layer that covers droplets. UVB exposure did not affect energies of droplet extension for species from exposed and partially to mostly shaded habitats (Argiope aurantia, Leucauge venusta and Verrucosa arenata). However, UVB exposure reduced the energy of droplet extension in Micrathena gracilis from shaded forests and Neoscona crucifera, which forages at night. Only in L. venusta did the energy of droplet extension increase after the dark treatment, suggesting endogenous molecular alignment. This study adds UVB irradiation to the list of factors (humidity, temperature and strain rate) known to affect the performance of spider glycoprotein glue, factors that must be more fully understood if adhesives that mimic spider glycoprotein glue are to be produced.

  15. Bone Glue Modified Asphalt: A Step towards Energy Conservation and Environment Friendly Modified Asphalts.

    Science.gov (United States)

    Rizvi, Hashim Raza; Khattak, Mohammad Jamal; Gallo, August A

    2014-01-01

    Asphalt has been modified for the past several decades using various additives, including synthetic polymers. Polymer modification improves structural and engineering characteristics of the binder, which is a result of improvement in rheological characteristics of binder as well as its adhesion capability with the aggregate. Such enhancement inevitably enhances the performance characteristics of hot mix asphalts (HMA) such as fatigue life, resistance to rutting, and thermal cracking. Even though polymer-modified HMA is popular in North America and European countries, its use is still limited in developing countries of Southeast Asia due to high costs associated with its manufacturing, processing, and energy consumption. In this study, a new kind of asphalt modifier derived from animal wastes, such as bones, hides, and flesh commonly known as Bone Glue, is studied. This biomaterial which is a by-product of food and cattle industries is cheap, conveniently available, and produced locally in developing countries. The results of the research study showed that the bone glue can easily be mixed with asphalt without significantly altering the asphalt binder's viscosity and mixing and compaction temperatures of HMA. Additionally, improvements in complex shear modulus for a range of temperatures were also determined and it was found that complex shear modulus was improved by bone glue modification.

  16. A direct biocombinatorial strategy toward next generation, mussel-glue inspired saltwater adhesives.

    Science.gov (United States)

    Wilke, Patrick; Helfricht, Nicolas; Mark, Andreas; Papastavrou, Georg; Faivre, Damien; Börner, Hans G

    2014-09-10

    Biological materials exhibit remarkable, purpose-adapted properties that provide a source of inspiration for designing new materials to meet the requirements of future applications. For instance, marine mussels are able to attach to a broad spectrum of hard surfaces under hostile conditions. Controlling wet-adhesion of synthetic macromolecules by analogue processes promises to strongly impact materials sciences by offering advanced coatings, adhesives, and glues. The de novo design of macromolecules to mimic complex aspects of mussel adhesion still constitutes a challenge. Phage display allows material scientists to design specifically interacting molecules with tailored affinity to material surfaces. Here, we report on the integration of enzymatic processing steps into phage display biopanning to expand the biocombinatorial procedure and enable the direct selection of enzymatically activable peptide adhesion domains. Adsorption isotherms and single molecule force spectroscopy show that those de novo peptides mimic complex aspects of bioadhesion, such as enzymatic activation (by tyrosinase), the switchability from weak to strong binders, and adsorption under hostile saltwater conditions. Furthermore, peptide-poly(ethylene oxide) conjugates are synthesized to generate protective coatings, which possess anti-fouling properties and suppress irreversible interactions with blood-plasma protein cocktails. The extended phage display procedure provides a generic way to non-natural peptide adhesion domains, which not only mimic nature but also improve biological sequence sections extractable from mussel-glue proteins. The de novo peptides manage to combine several tasks in a minimal 12-mer sequence and thus pave the way to overcome major challenges of technical wet glues.

  17. Composition and Function of Spider Glues Maintained During the Evolution of Cobwebs.

    Science.gov (United States)

    Jain, Dharamdeep; Zhang, Ci; Cool, Lydia Rose; Blackledge, Todd A; Wesdemiotis, Chrys; Miyoshi, Toshikazu; Dhinojwala, Ali

    2015-10-12

    Capture silks are an interesting class of biological glues that help spiders subdue their prey. Viscid capture silk produced by the orb web spiders is a combination of hygroscopic salts that aid in water uptake and interact with adhesive glycoproteins to make them soft and sticky. The orb was a stepping stone to the evolution of new web types, but little is known about the adhesives in these webs. For instance, cobweb spiders evolved from orb-weaving ancestors and utilize glue in specialized sticky gumfoot threads rather than an elastic spiral. Early investigation suggests that gumfoot adhesives are quite different viscid glues because they lack a visible glycoprotein core, act as viscoelastic fluids rather than solids, and are largely invariant to humidity. Here, we use spectroscopic and staining methods to show that the gumfoot silk produced by Latrodectus hesperus (western black widow) is composed of hygroscopic organic salts and water insoluble glycoproteins, similar to viscid silk, in addition to a low concentration of spider coating peptides reported before. Our adhesion studies reveal that the organic salts play an important role in adhesion, similar to that seen in orb web spiders, but modulating function at much lower humidity. Our work shows more similarities in the viscid silk produced by orb web and cobweb spiders than previously anticipated and provide guidelines for developing synthetic adhesives that can work in dry to humid environments.

  18. The interplay between tissue plasminogen activator domains and fibrin structures in the regulation of fibrinolysis: kinetic and microscopic studies.

    Science.gov (United States)

    Longstaff, Colin; Thelwell, Craig; Williams, Stella C; Silva, Marta M C G; Szabó, László; Kolev, Krasimir

    2011-01-13

    Regulation of tissue-type plasminogen activator (tPA) depends on fibrin binding and fibrin structure. tPA structure/function relationships were investigated in fibrin formed by high or low thrombin concentrations to produce a fine mesh and small pores, or thick fibers and coarse structure, respectively. Kinetics studies were performed to investigate plasminogen activation and fibrinolysis in the 2 types of fibrin, using wild-type tPA (F-G-K1-K2-P, F and K2 binding), K1K1-tPA (F-G-K1-K1-P, F binding), and delF-tPA (G-K1-K2-P, K2 binding). There was a trend of enzyme potency of tPA > K1K1-tPA > delF-tPA, highlighting the importance of the finger domain in regulating activity, but the differences were less apparent in fine fibrin. Fine fibrin was a better surface for plasminogen activation but more resistant to lysis. Scanning electron and confocal microscopy using orange fluorescent fibrin with green fluorescent protein-labeled tPA variants showed that tPA was strongly associated with agglomerates in coarse but not in fine fibrin. In later lytic stages, delF-tPA-green fluorescent protein diffused more rapidly through fibrin in contrast to full-length tPA, highlighting the importance of finger domain-agglomerate interactions. Thus, the regulation of fibrinolysis depends on the starting nature of fibrin fibers and complex dynamic interaction between tPA and fibrin structures that vary over time.

  19. Treatment of gingival recession using free gingival graft with fibrin fibronectin sealing system: A novel approach.

    Science.gov (United States)

    Srinivas, B V V; Rupa, N; Halini Kumari, K V; Rajender, A; Reddy, M Narendra

    2015-08-01

    Periodontal plastic surgery is the branch of periodontology that is focused mainly on the correction or elimination of mucogingival problems associated with lack of attached gingiva, a shallow vestibule and aberrant frenum. Various mucogingival surgical procedures are used to halt the progression of the gingival recession and to correct poor esthetic appearance. Free gingival autograft is one of the most common techniques used for a gingival recession in areas of inadequate attached gingiva in the mandibular anterior region. Fibrin sealants are human plasma derivatives that mimic the final stages of blood coagulation, forming a fibrin clot. Fibrin Sealants enhances the overall outcome of surgical intervention because of their hemostatic, adhesive, and healing properties. These properties of fibrin sealants may reduce operating time, prevent complications, and enhance the overall outcome of many surgical interventions. Hence, this case report aims to investigate the clinical effectiveness of free gingival graft along with the commercially available fibrin-fibronectin sealing system (Tissucol(®)) in the treatment of Miller's class II gingival recession.

  20. Human umbilical cord stem cell encapsulation in novel macroporous and injectable fibrin for muscle tissue engineering

    Science.gov (United States)

    Liu, Jun; Xu, Hockin H.K.; Zhou, Hongzhi; Weir, Michael D.; Chen, Qianming; Trotman, Carroll Ann

    2012-01-01

    There has been little research on the seeding of human umbilical cord mesenchymal stem cells (hUCMSCs) in three-dimensional scaffolds for muscle tissue engineering. The objectives of this study were: (i) to seed hUCMSCs in a fibrin hydrogel containing fast-degradable microbeads (dMBs) to create macropores to enhance cell viability; and (ii) to investigate the encapsulated cell proliferation and myogenic differentiation for muscle tissue engineering. Mass fractions of 0–80% of dMBs were tested, and 35% of dMBs in fibrin was shown to avoid fibrin shrinkage while creating macropores and promoting cell viability. This construct was referred to as “dMB35”. Fibrin without dMBs was termed “dMB0”. Microbead degradation created macropores in fibrin and improved cell viability. The percentage of live cells in dMB35 reached 91% at 16 days, higher than the 81% in dMB0 (p muscle tissue engineering applications. PMID:22902812

  1. Excess Fibrin Deposits Decrease Fetal Weight of Pregnant Mice Infected by Plasmodium berghei

    Directory of Open Access Journals (Sweden)

    Desy Andari

    2014-05-01

    Full Text Available Low birth weight is commonly attributed to malaria in pregnancy, but the cellular and molecular mechanisms that underlie this poor birth outcome are incompletely understood. A universally described histopathological feature of placental malaria is excessive deposition of fibrin, the end-product of the coagulation cascade. This study was conducted to compare fibrin deposit in pregnant mice that infected by Plasmodium berghei (treatment group to the normal pregnant mice (control group and its association with fetal weight. This research is in vivo experimental laboratory study that used 18 pregnant Balb/c mice which divided to the control the group (8 mice and treatment group (9 mice infected by P.berghei. Placentas were staining with Haematoxylin-Eosin (HE for fibrin deposits examination whereas fetal weight was performed with Mettler analytical balance AE 50. Fetal weight of the treatment group was lower than those of the control group (t test, p=0,002. Fibrin deposits were increased in the treatment group (t test, p=0,005 and influenced weight of fetuses (Spearman r= -0,586, p= 0,014. Weights of fetuses are interfered by fibrin deposits during malaria infection.

  2. The application of plastic compression to modulate fibrin hydrogel mechanical properties.

    Science.gov (United States)

    Haugh, Matthew G; Thorpe, Stephen D; Vinardell, Tatiana; Buckley, Conor T; Kelly, Daniel J

    2012-12-01

    The inherent biocompatibility of fibrin hydrogels makes them an attractive material for use in a wide range of tissue engineering applications. Despite this, their relatively low stiffness and high compliance limits their potential for certain orthopaedic applications. Enhanced mechanical properties are desirable so as to withstand surgical handling and in vivo loading after implantation and additionally, can provide important cues to cells seeded within the hydrogel. Standard methods used to enhance the mechanical properties of biological scaffolds such as chemical or thermal crosslinking cannot be used with fibrin hydrogels as cell seeding and gel formation occurs simultaneously. The objective of this study was to investigate the use of plastic compression as a means to improve the mechanical properties of chondrocyte-seeded fibrin hydrogels and to determine the influence of such compression on cell viability within these constructs. It was found that the application of 80% strain to fibrin hydrogels for 30 min (which resulted in a permanent strain of 47.4%) produced a 2.1-fold increase in the subsequent compressive modulus. Additionally, chondrocyte viability was maintained in the plastically compressed gels with significant cellular proliferation and extracellular matrix accumulation observed over 28 days of culture. In conclusion, plastic compression can be used to modulate the density and mechanical properties of cell-seeded fibrin hydrogels and represents a useful tool for both in theatre and in vitro tissue engineering applications.

  3. [Propolis. The bee glue as presented by the Graeco-Roman literature].

    Science.gov (United States)

    Golder, Werner

    2004-01-01

    The bee glue, commonly known as propolis, has been employed for medical purposes already in teh ancient world. More than 15 Greek and Roman authors report on the preparation and application of the so-called third natural product of the bees (besides honey and wax). Aristoteles described the fundamental issues of its biology in his 'Historia Animalium' correctly. The bulk of propolis is obtained from the barks of poplars. Once carried in the hives, the glue is used to stabilize the cells and honeycombs and to protect the bees against invaders and cold weather. Propolis has been chiefly employed for the preparation of ointment and plasters. For this purpose, the viscous raw material was purified, moulded and boiled. In most preparations, the bee glue was only one of many (up to 20) pharmacologically active constituents and came to five to 20% of the mixture. Only rarely, a single drug therapy was using propolis was carried out. The application of the glue was most successful in general surgery and casualties. In that respect, the ancient physicians took advantage of the anti-edematous and anti-infectious properties of the substance. Thus, it was used to treat bumps, indurations, and slow-healing wounds. Moreover, cataplasms against swollen cervical nodes and indurations of the female breast often contained propolis. Finally, bee glue proved successful for the treatment of chronic backache and pain in the hip as well as fresh injuries of muscles and tendons. In the sector of skin diseases, lichens and condylomata were found to respond well to propolis. ALl this indications have been a matter of several records. However, the successful use of propolis in diseases of the stomach and liver has ben reported solely by Alexander of Tralles (6th century AD). Not counting the internal diseases, the spectrum of indications for propolis has not substantially changed as compared to the classical antiquity. Interestingly, radiation therapists have adopted the ancient remedy and

  4. Evaluation of cosmetic appearance of herniotomy wound scars in African children: Comparison of tissue glue and subcuticular suturing

    Directory of Open Access Journals (Sweden)

    Ademuyiwa A

    2009-01-01

    Full Text Available Aim: To evaluate the cosmetic appearance of herniotomy wound scars closed using either the tissue glue or subcuticular suturing technique. Materials and Methods: Prospective randomised control study; randomisation into tissue glue and suturing groups. Ethical clearance obtained. Cosmetic outcome were based on visual analogue scale by parents and Hollander wound evaluation scale by a Plastic Surgeon blinded to the wound closure method. Results: Fifty one wounds were evaluated, 26 in the tissue glue group and 25 in the suturing group. Parents′ evaluation using Visual Analogue scale (VAS showed that in the suturing group, 17 parents (68% gave a VAS of 8cm while six parents (24% gave a score of 7cm. Two parents (8% gave a score of 9cm. In the tissue glue group, 22 parents (84.6% scored the scar of their children as 8 or 9cm on the VAS while four parents (15.4% gave a score of 7cm. The median VAS was 8cm for both groups with a range of 7 to 9cm. The Chi- square test showed that the parents preferred tissue glue compared with subcuticular suturing (X2 = 7.90, P < 0.05. The Hollander Wound Evaluation Scale (HWES used by Plastic Surgeon showed 21 herniotomy wounds (84% had a score of 6 in the suturing group while four wounds (16% had a score of 5. In the tissue glue group, 19 wounds (73% had a score of 6, six wounds (23.1% had a score of 5 and a patient (3.8% had a score of 4. The median score is 6 for both groups. There was no statistically significant difference between both groups (X 2 = 1.481, P = 0.393. Conclusion: This study has shown that the cosmetic outcome of wound closure using the tissue glue technique and subcuticular suturing technique are similar.

  5. Bortezomib consolidation after autologous stem cell transplantation in multiple myeloma

    DEFF Research Database (Denmark)

    Mellqvist, Ulf-Henrik; Gimsing, Peter; Hjertner, Oyvind

    2013-01-01

    The Nordic Myeloma Study Group conducted an open randomized trial to compare bortezomib as consolidation therapy given after high-dose therapy and autologous stem cell transplantation (ASCT) with no consolidation in bortezomib-naive patients with newly diagnosed multiple myeloma. Overall, 370...

  6. Lack of autologous tissue transmission of eosinophilic plaques in cats.

    Science.gov (United States)

    Moriello, K A; Kunkle, G; Miller, L M; Crowley, A

    1990-07-01

    Autologous tissue transmission of spontaneously developing feline eosinophilic plaques was attempted in 5 cats. Macerated tissue from the plaque was vigorously rubbed onto 2 scarified skin sites in each cat. The inoculated areas were observed daily for 30 days. During that time, no clinical or histologic evidence of transmission was found.

  7. Experimental autologous substitute vascular graft for transplantation surgery

    NARCIS (Netherlands)

    Kobori, L; Dallos, G; Gouw, ASH; Nemeth, T; Nemes, B; Fehervari, I; Tegzess, Adam; Slooff, MJH; Perner, F; De Jong, KP

    2000-01-01

    Vascular complications in fiver transplantation are a major cause of graft failure and mortality. The aim of the study was to create autologous vascular graft without risk of rejection. Posterior rectus fascia sheath lined with peritoneum was used for iliac artery replacement in seven mongrel dogs.

  8. Immunisation of colorectal cancer patients with autologous tumour cells

    DEFF Research Database (Denmark)

    Diederichsen, Axel Cosmus Pyndt; Stenholm, A C; Kronborg, O

    1998-01-01

    Patients with colorectal cancer were entered into a clinical phase I trial of immunotherapy with an autologous tumour cell/bacillus Calmette-Guerin (BCG) vaccine. We attempted to describe the possible effects and side effects of the immunisation, and further to investigate whether expression...

  9. Do autologous blood and PRP injections effectively treat tennis elbow?

    Science.gov (United States)

    Widstrom, Luke; Slattengren, Andrew

    2016-09-01

    Both approaches reduce pain, but the improvement with platelet-rich plasma (PRP) is not clinically meaningful. Autologous blood injections (ABIs) are more effective than corticosteroid injections for reducing pain and disability in patients with tennis elbow in both the short and long term.

  10. A Role For Photodynamic Therapy In Autologous Bone Marrow Transplantation

    Science.gov (United States)

    Sieber, Fritz

    1988-02-01

    Simultaneous exposure to the amphipathic fluorescent dye merocyanine 540 (MC 540) and light of a suitable wavelength rapidly kills leukemia, lymphoma, and neuroblastoma cells but spares normal pluripotent hematopoietic stem cells. Tests in several preclinical models and early results of a phase I clinical trial suggest that MC 540-mediated photosensitization may be useful for the extracorporeal purging of autologous remission bone marrow grafts.

  11. Autologous serum for anterior tissue necrosis after porous orbital implant

    Directory of Open Access Journals (Sweden)

    Saurabh Kamal

    2014-01-01

    Full Text Available Orbital implants are now routinely used after enucleation and evisceration. However exposure of the implant can lead to infection and extrusion. Hence, early repair of larger exposure with graft material is required. We describe three cases where early postoperative mucosal dehiscence was successfully managed with autologous serum.

  12. Hematopoietic progenitor cell mobilization for autologous transplantation - a literature review

    Directory of Open Access Journals (Sweden)

    Marco Aurélio Salvino

    2016-02-01

    Full Text Available ABSTRACT The use of high-dose chemotherapy with autologous support of hematopoietic progenitor cells is an effective strategy to treat various hematologic neoplasms, such as non-Hodgkin lymphomas and multiple myeloma. Mobilized peripheral blood progenitor cells are the main source of support for autologous transplants, and collection of an adequate number of hematopoietic progenitor cells is a critical step in the autologous transplant procedure. Traditional strategies, based on the use of growth factors with or without chemotherapy, have limitations even when remobilizations are performed. Granulocyte colony-stimulating factor is the most widely used agent for progenitor cell mobilization. The association of plerixafor, a C-X-C Chemokine receptor type 4 (CXCR4 inhibitor, to granulocyte colony stimulating factor generates rapid mobilization of hematopoietic progenitor cells. A literature review was performed of randomized studies comparing different mobilization schemes in the treatment of multiple myeloma and lymphomas to analyze their limitations and effectiveness in hematopoietic progenitor cell mobilization for autologous transplant. This analysis showed that the addition of plerixafor to granulocyte colony stimulating factor is well tolerated and results in a greater proportion of patients with non-Hodgkin lymphomas or multiple myeloma reaching optimal CD34+ cell collections with a smaller number of apheresis compared the use of granulocyte colony stimulating factor alone.

  13. How effective is autologous serum therapy in chronic autoimmune urticaria

    Directory of Open Access Journals (Sweden)

    Imran Majid

    2015-01-01

    Full Text Available Background: Chronic autoimmune urticaria (CAU is one of the most challenging therapeutic problems faced by a dermatologist. Recently, weekly autologous serum injections have been shown to induce a prolonged remission in this disease. Aim: To evaluate the efficacy of repeated autologous serum injections in patients with CAU. Materials and Methods: Seventy patients of CAU were prospectively analyzed for the efficacy of nine consecutive weekly autologous serum injections with a post-intervention follow-up of 12 weeks. Total urticaria severity score (TSS was monitored at the baseline, at the end of treatment and lastly at the end of 12 weeks of follow up. Response to treatment was judged by the percentage reduction in baseline TSS at the end of treatment and again at the end of 12 weeks-follow-up. Results: Out of the 70 patients enrolled, 11 dropped out of the injection treatment after one or the first few doses only. Among the rest of 59 patients, only 7 patients (12% went into a partial or complete remission and remained so over the follow-up period of 12 weeks. Forty patients (68% did not demonstrate any significant reduction in TSS at the end of the treatment period. Rest of the 12 patients showed either a good or excellent response while on weekly injection treatment, but all of them relapsed over the follow-up period of 12 weeks. Conclusion: Autologous serum therapy does not seem to lead to any prolonged remission in patients of CAU.

  14. Osteoarthritis treatment using autologous conditioned serum after placebo

    NARCIS (Netherlands)

    Rutgers, Marijn; Creemers, Laura B; Auw Yang, Kiem Gie; Raijmakers, Natasja J H; Dhert, Wouter J A; Saris, Daniel B F

    2015-01-01

    BACKGROUND AND PURPOSE: Autologous conditioned serum (ACS) is a disease-modifying drug for treatment of knee osteoarthritis, and modest superiority over placebo was reported in an earlier randomized controlled trial (RCT). We hypothesized that when given the opportunity, placebo-treated patients fro

  15. AUTOLOGOUS VEIN SUPPORTED WITH A BIODEGRADABLE PROSTHESIS FOR ARTERIAL GRAFTING

    NARCIS (Netherlands)

    ZWEEP, HP; SATOH, S; VANDERLEI, B; HINRICHS, WLJ; DIJK, F; FEIJEN, J; WILDEVUUR, CRH

    1993-01-01

    To evaluate the potential of a supporting, compliant, biodegradable prosthesis to function as a temporary protective scaffold for autologous vein grafts in the arterial circulation, we implanted vein grafts into the carotid arteries of rabbits, either with (composite grafts) or without (control graf

  16. Autologous tissue repair of large abdominal wall defects.

    NARCIS (Netherlands)

    Vries Reilingh, T.S. de; Bodegom, M.E.; Goor, H. van; Hartman, E.H.M.; Wilt, G.J. van der; Bleichrodt, R.P.

    2007-01-01

    BACKGROUND AND METHOD: Techniques for autologous repair of abdominal wall defects that could not be closed primarily are reviewed. Medline and PubMed were searched for English or German publications using the following keywords: components separation technique (CST), Ramirez, da Silva, fascia lata,

  17. Fibrin Sealant for Prevention of Resection Surface-Related Complications After Liver Resection A Randomized Controlled Trial

    NARCIS (Netherlands)

    de Boer, Marieke T.; Klaase, Joost M.; Verhoef, Cornelis; van Dam, Ronald M.; van Gulik, Thomas M.; Molenaar, I. Quintus; Bosscha, Koop; Dejong, Cornelis H. C.; Van der Jagt, Eric J.; Porte, Robert J.

    2012-01-01

    Objective: To evaluate the efficacy of fibrin sealant in reducing resection surface-related complications in liver surgery. Background: Bile leakage, bleeding, and abscess formation are major resection surface-related complications after liver resection. It is unclear whether application of fibrin s

  18. Modulation of 3D Fibrin Matrix Stiffness by Intrinsic Fibrinogen–Thrombin Compositions and by Extrinsic Cellular Activity

    Science.gov (United States)

    Duong, Haison; Wu, Benjamin

    2009-01-01

    Fibrin is a substance formed through catalytic conversion of coagulation constituents: fibrinogen and thrombin. The kinetics of the two constituents determines the structural properties of the fibrin architecture. We have shown previously that changing the fibrinogen and thrombin concentrations in the final three-dimensional (3D) fibrin matrix influenced cell proliferation and differentiation. In this study, we further examined the effect of changing fibrinogen and thrombin concentrations in the absence or presence of fibroblasts on the structural modulus or stiffness of 3D fibrin matrices. We have prepared fibroblast-free and fibroblast-embedded 3D fibrin matrices of different fibrinogen and thrombin formulations, and tested the stiffness of these constructs using standard mechanical testing assays. Results showed that there was a corresponding increase in stiffness with increasing thrombin and fibrinogen concentrations; the increase was more notable with fibrinogen and to a lesser degree with thrombin. The effect of fibroblasts on the stiffness of the fibrin construct was also examined. We have observed a small increase in the stiffness of the fibroblast-incorporated fibrin construct as they proliferated and exhibited spreading morphology. To our knowledge, this is the first comprehensive report detailing the relationship between fibrinogen and thrombin concentrations, cell proliferation, and stiffness in 3D fibrin matrices. The data obtained may lead to optimally design suitable bioscaffolds where we can control both cell proliferation and structural integrity for a variety of tissue engineering applications. PMID:19309239

  19. Updates of Choukroun's platelet-rich fibrin in dental implant bone defect%Choukroun富血小板纤维蛋白在口腔种植骨缺损中的研究与进展

    Institute of Scientific and Technical Information of China (English)

    许丰伟; 柳忠豪

    2012-01-01

    背景:近年来,Choukroun富血小板纤维蛋白作为一种富含自体细胞因子和生长因子的新型生物材料,在口腔种植骨缺损的临床应用中受到了广泛关注.目的:就富血小板纤维蛋白的实验及临床研究进展进行综述.方法:由第一作者应用计算机检索PubMed数据库及中国期刊网全文数据库2000-01/2011-12有关富血小板纤维蛋白生物学特性、实验研究以及临床应用等方面的文章,英文检索词为"choukroun's platelet-rich fibrin,platelet-rich plasma,dental implant,bone defect,GBR",中文检索词为"富血小板纤维蛋白,富血小板血浆,口腔种植,骨缺损,引导骨再生".排除重复性研究,共保留30篇文献进行综述.结果与结论:富血小板纤维蛋白由纤维蛋白网、血小板及粒细胞等组成,是目前较为先进的一种富含成骨作用因子的血液制品.具有制备过程操作简单、不需要添加其他制剂的特点,同时还有效模拟生理状态下血凝块中纤维蛋白的形成,与人体正常血凝块中的纤维蛋白相似,并很好地避免了免疫排斥和交叉感染的发生.在实验研究及临床应用中均显示了很好的促进组织愈合的能力.加之其成本低廉、取材方便等优点,在今后的口腔种植领域将得到越来越多的关注.%BACKGROUND: Recently, Choukroun's platelet-rich fibrin (PRF) is known as a rich source of autologous cytokines and growth factors, and special attention is paid as a new biomaterial in dental implant bone defect application.OBJECTIVE: To review experiments and clinical researches of the PRF.METHODS: A computer-based retrieval was performed by the first author in the CNKI and PubMed databases to search papers published between January 2000 and December 2011 with the key words of “Choukroun's platelet-rich fibrin, Platelet-rich plasma,dental implant, bone defect, GBR” in Chinese and English. According to inclusion and exclusion criteria, 30 papers were

  20. Regeneration of Tissues and Organs Using Autologous Cells

    Energy Technology Data Exchange (ETDEWEB)

    Anthony Atala

    2010-04-28

    The Joint Commission for Health Care Organizations recently declared the shortage of transplantable organs and tissues a public health crisis. As such, there is about one death every 30 seconds due to organ failure. Complications and rejection are still significant albeit underappreciated problems. It is often overlooked that organ transplantation results in the patient being placed on an immune suppression regimen that will ultimate shorten their life span. Patients facing reconstruction often find that surgery is difficult or impossible due to the shortage of healthy autologous tissue. In many cases, autografting is a compromise between the condition and the cure that can result in substantial diminution of quality of life. The national cost of caring for persons who might benefit from engineered tissues or organs has reached $600 billion annually. Autologous tissue technologies have been developed as an alternative to transplantation or reconstructive surgery. Autologous tissues derived from the patient's own cells are capable of correcting numerous pathologies and injuries. The use of autologous cells eliminates the risks of rejection and immunological reactions, drastically reduces the time that patients must wait for lifesaving surgery, and negates the need for autologous tissue harvest, thereby eliminating the associated morbidities. In fact, the use of autologous tissues to create functional organs is one of the most important and groundbreaking steps ever taken in medicine. Although the basic premise of creating tissues in the laboratory has progressed dramatically, only a limited number of tissue developments have reached the patients to date. This is due, in part, to the several major technological challenges that require solutions. To that end, we have been in pursuit of more efficient ways to expand cells in vitro, methods to improve vascular support so that relevant volumes of engineered tissues can be grown, and constructs that can mimic the

  1. 纤维蛋白胶介导内皮祖细胞再生心肌梗死血管的可行性%Myocardial revascularization after myocardial infarction using endothelial progenitor cells combined with fibrin gel

    Institute of Scientific and Technical Information of China (English)

    阿迪拉·阿扎提; 赵龙; 周欣荣; 刘芬; 陈邦党; 马依彤

    2014-01-01

    背景:有研究显示纤维蛋白胶可促进成肌细胞移植物的保持和生存,减少梗死范围并在梗死区诱导新生血管化。目的:了解内皮祖细胞经可降解材料纤维蛋白胶移植到大鼠梗死心肌后的血管再生情况。方法:将27只SD大鼠随机均分为3组,非心肌梗死组9只、心肌梗死即刻移植组9只与心肌梗死1周移植组9只。每个大组又再分为两个亚组,即移植人脐带源内皮祖细胞-纤维蛋白胶复合物的实验组与移植纤维蛋白胶的对照组。移植后3,8周处死,通过显微镜、免疫组织化学和心脏超声观察其在梗死心肌的血管再生和心功能改善情况。结果与结论:显微镜观察到,实验组大鼠心脏和胸部之间有一些疏松的结缔组织,而其与对照组之间无明显差异。组织和免疫学观察发现,各实验组和对照组的心脏结构不易区分且相对正常,未发现血管瘤、血管畸形和肿瘤等。血管测量结果显示实验组和对照组之间,以及各实验组之间均无差异,并且实验组和对照组之间心功能检查也没有统计学意义。此次研究内皮祖细胞结果没有阳性表现,将修改并提高细胞通过纤维蛋白基质传递的方法策略,确信细胞传递系统提供的有益性和有效性将会进一步得到证实。%BACKGROUND:Studies have shown that fibrin glue can promote the survival of myoblast grafts, reduce infarct size and induce neovascularization of infarct zone. OBJECTIVE:To understand the condition of revascularization of infarcted heart muscle using endothelial progenitor cells combined with degradable fibrin glue materials. METHODS:A total of 27 Sprague-Dawley rats were randomized into three groups, 9 rats in each group:non-myocardial infarction group, immediate transplantation group and 1-week post-infarction transplantation group. Then, these three groups were sub-grouped into two groups, respectively

  2. Shoulder surgeon and autologous cellular regeneration - From bench to bed: Part one- the link between the human fibroblast, connective tissue disorders and shoulder

    Directory of Open Access Journals (Sweden)

    Du Toit Don

    2007-01-01

    Full Text Available The process of autologous cell regeneration (ACR is a facet of cell therapy and regenerative medicine. It is initiated when activated autologous platelet rich plasma alone (PRP, containing cytokines or growth factors, is injected into the dermis or other structure in order to initiate a regenerative or antiaging process. The recipients resident cells at the target zone are activated by biologically active growth factors, derived from the activated platelets in the PRP through a paracrine effect. The platelet gel that contains the fibrin and clumped platelets, releases growth factors that influences activation of macrophages and stem cells in the recipient site. Tissue regeneration is facilitated by stem cell proliferation and differentiation. The PRP that has sealant and wound healing properties, may shift the wound healing cascade to the left, thereby speeding up tissue regeneration and remodelling by the use of the recipients own plasma. Both the fibroblast and myofibroblast play key roles in the wound healing cascade. The fibroblast, of mesenchymal origin, plays a pivotal role in the formation of the extracellular matrix and deposition of collagen. A failure of organised fibroblast function results in important and disabling disease processes and conditions such as chronic ischaemic heart disease and remodelling of the heart, lung fibrosis, fibromatosis, solar aged-face, keloids, hypertrophic scar formation, nodular fasciitis, inguinal hernia, Dupuytren′s disease and scleroderma. More recent additions include frozen shoulder and the captured shoulder. In aesthetic medicine, one of the prime functions of fractional photothermolysis with lasers, intense pulsed light and radiofrequency devices, is to stimulate dermal fibroblast proliferation, by thermal energy thereby increasing collagen deposition which enhances facial rejuvenation. Platelet -rich plasma (REGENLAB PRP, REGENLAB-ACR possesses unique growth factors that stimulate, fibroblast

  3. [Results of fibrin clot application for acceleration of regeneration of the damaged mandible in experiment].

    Science.gov (United States)

    Maĭborodin, I V; Kolesnikov, I S; Shevela, A I; Sheplev, B V; Drovosekov, M N; Toder, M S

    2011-01-01

    The processes of regeneration of the damaged rat bottom jaw bone after application of enriched thrombocytes a fibrin clot were studied by morphological and radiovisiographic methods. At a natural course of regeneration the artificial aperture of bone was filled with blood and there the blood clot was formed. After 1 week the separate bone islets of a young tissue occurred in bone defect. In 2-3 weeks the aperture in a bottom jaw bone was completely closed by a young bone tissue. After operation with filling of bone bottom jaw defect by fibrin clot there was no formation of a blood clot. Already after 1 week the bone tissue defect was filled by the merged islets of again generated bone. By second week after fibrin use the further formation of bone tissue in defect and formation of a bone callosity was noted.

  4. Crosslinked fibrin gels for tissue engineering: two approaches to improve their properties.

    Science.gov (United States)

    Gamboa-Martínez, Tatiana C; Luque-Guillén, Victoria; González-García, Cristina; Gómez Ribelles, José Luis; Gallego-Ferrer, Gloria

    2015-02-01

    Fibrin is a protein that can be used as an ideal scaffolding material to promote tissue regeneration. In order to enhance its physical properties in this study a natural crosslinker, genipin (GP), was employed with the aim to obtain a hydrogel with tuneable properties for tissue engineering applications. The fibrin gel was crosslinked by two different methods using four concentrations of GP to get a stable hydrogel network. Crosslinking density, mechanical properties, swelling, and enzymatic degradation of the hydrogels were tested for each GP content and method employed. The method I: Crosslinking after gel formation promotes a high crosslinking and retains the gel shape for long term whilst the method II: Simultaneous gel formation and crosslinking improves the mechanical properties of the gel. This study confirms the use of GP at different concentrations as a suitable crosslinker of fibrin that promotes the cellular viability of L929 for 21 days of in vitro culture.

  5. Role of platelet-rich-fibrin in enhancing palatal wound healing after free graft

    Directory of Open Access Journals (Sweden)

    Vinita Jain

    2012-01-01

    Full Text Available Platelet-rich fibrin has long been used as a wound healing therapy in skin wounds and recently evidence has suggested its usage in oral cavity for different treatment procedures. This article proposes an overview of use of platelet-rich fibrin in management of complicated oral wounds. Excessive hemorrhage of the donor area, necrosis of epithelium, and morbidity associated with donor site have been described as the possible complications after harvesting subepithelial connective tissue graft, but little has been mentioned about their management. The article includes a case report of a 45-year-old male patient who showed a delayed wound healing after subepithelial connective tissue graft harvestation, which was treated with platelet-rich fibrin.

  6. EFFECTIVENESS OF AUTOLOGOUS SERUM THERAPY IN CHRONIC URTICARIA: A PROSPECTIVE OBSERVATIONAL STUDY IN TERTIARY CARE HOSPITAL

    Directory of Open Access Journals (Sweden)

    Rakesh Kumar

    2016-02-01

    Full Text Available BACKGROUND Chronic urticaria is a challenging condition, both for patient and physician in a day-to-day practice. There is constant search for newer modality of treatment, which can provide prolong remission with less side effect. Autologous serum therapy have shown promising result in initial studies. AIM To evaluate effectiveness of Autologous Serum Therapy in chronic urticaria patients. MATERIALS AND METHODS A prospective observational study. Total 220 patients enrolled for study. Autologous serum skin test performed in all patients; 113 patients were given Autologous Serum Therapy along with oral levocetirizine on SOS basis weekly and 107 patients were given only oral levocetirizine on demand basis. Response to treatment assessed by urticaria activity score, urticaria total severity score, antihistamine score, dermatological quality of life index and Likert scale on 2 weekly interval for 10 weeks. RESULTS Autologous Serum Therapy shown significant improvement in both Autologous Serum Skin Test positive and Autologous Serum Skin Test negative patients as compared to non-Autologous Serum Therapy groups. Autologous Serum Therapy is more effective in Autologous Serum Skin Test positive patients. CONCLUSION Autologous Serum Therapy is effective in chronic urticaria patients.

  7. El fibrinógeno como factor de riesgo de enfermedad aterotrombótica

    OpenAIRE

    Julio César Fernández Travieso

    2009-01-01

    La disfunción endotelial y la inflamación representan un decisivo papel desde las etapas tempranas de la aterosclerosis hasta la aparición de los síndromes coronarios agudos, en los cuales el fibrinógeno ha demostrado tener una activa participación. Además, en concentraciones elevadas se relaciona con la trombosis y ha sido demostrado que es un factor independiente de riesgo cardiovascular. Hoy día, concentraciones elevadas de fibrinógeno junto con la proteína C-reactiva, la lipoproteína (a) ...

  8. Use of platelet-rich fibrin over skin wounds: Modified secondary intention healing

    Directory of Open Access Journals (Sweden)

    Chirag B Desai

    2013-01-01

    Full Text Available The healing process after any surgical intervention has always posed a challenge for the surgeons. In spite of the advances in wound closure techniques and devices, there is a crucial need for newer methods of enhancing the healing process to achieve optimal outcomes. Fibrin adhesives and platelet concentrates have proven useful in various treatment modalities in the fields of microvascular and plastic surgery. This case report shows its unique use in the field of maxillofacial and cutaneous surgery. It shows an innovative technique of enhancement of skin wound healing by local application of platelet-rich fibrin.

  9. Introgressive hybridization and evolution of a novel protein phenotype: glue protein profiles in the nasuta–albomicans complex of Drosophila

    Indian Academy of Sciences (India)

    S. Aruna; H. A. Ranganath

    2006-04-01

    Glue proteins are tissue-specific proteins synthesized by larval salivary gland cells of Drosophila. In Drosophila nasuta nasuta and D. n. albomicans of the nasuta subgroup, the genes that encode the major glue protein fractions are X-linked. In the present study, these X-linked markers have been employed to trace the pattern of introgression of D. n. nasuta and D. n. albomicans genomes with respect to the major glue protein fractions in their interracial hybrids, called cytoraces. These cytoraces have inherited the chromosomes of both parents and have been maintained in the laboratory for over 400–550 generations. The analysis has revealed that cytoraces with D. n. albomicans X chromosome show either D. n. nasuta pattern or a completely novel pattern of glue protein fractions. Further, quantitative analysis also shows lack of correlation between the chromosomal pattern of inheritance and overall quantity of the major glue protein fractions in the cytoraces. Thus, in cytoraces the parental chromosomes are not just differentially represented but there is evidence for introgression even at the gene level.

  10. Repair of Segmental Load-Bearing Bone Defect by Autologous Mesenchymal Stem Cells and Plasma-Derived Fibrin Impregnated Ceramic Block Results in Early Recovery of Limb Function

    Directory of Open Access Journals (Sweden)

    Min Hwei Ng

    2014-01-01

    Full Text Available Calcium phosphate-based bone substitutes have not been used to repair load-bearing bone defects due to their weak mechanical property. In this study, we reevaluated the functional outcomes of combining ceramic block with osteogenic-induced mesenchymal stem cells and platelet-rich plasma (TEB to repair critical-sized segmental tibial defect. Comparisons were made with fresh marrow-impregnated ceramic block (MIC and partially demineralized allogeneic bone block (ALLO. Six New Zealand White female rabbits were used in each study group and three rabbits with no implants were used as negative controls. By Day 90, 4/6 rabbits in TEB group and 2/6 in ALLO and MIC groups resumed normal gait pattern. Union was achieved significantly faster in TEB group with a radiological score of 4.50 ± 0.78 versus ALLO (1.06 ± 0.32, MIC (1.28 ± 0.24, and negative controls (0. Histologically, TEB group scored the highest percentage of new bone (82% ± 5.1% compared to ALLO (5% ± 2.5% and MIC (26% ± 5.2%. Biomechanically, TEB-treated tibiae achieved the highest compressive strength (43.50 ± 12.72 MPa compared to those treated with ALLO (15.15 ± 3.57 MPa and MIC (23.28 ± 6.14 MPa. In conclusion, TEB can repair critical-sized segmental load-bearing bone defects and restore limb function.

  11. Glueballs amass at RHIC and LHC Colliders! - The early quarkless 1st order phase transition at $T=270$ MeV - from pure Yang-Mills glue plasma to GlueBall-Hagedorn states

    CERN Document Server

    Stoecker, Horst; Schramm, Stefan; Senzel, Florian; Greiner, Carsten; Beitel, Maxim; Gallmeister, Kai; Gorenstein, Mark; Mishustin, Igor; Vasak, David; Steinheimer, Jan; Struckmeier, Juergen; Vovchenko, Volodymyr; Satarov, Leonid; Xu, Zhe; Zhuang, Pengfei; Csernai, Laszlo P; Sinha, Bikash; Raha, Sibaji; Biró, Tamás Sándor; Panero, Marco

    2016-01-01

    The early stage of high multiplicity pp, pA and AA collider is represented by a nearly quarkless, hot, deconfined pure gluon plasma. According to pure Yang-Mills Lattice Gauge Theory, this hot pure glue matter undergoes, at a high temperature, $T_c = 270$ MeV, a first order phase transition into a confined Hagedorn-GlueBall fluid. These new scenario should be characterized by a suppression of high $p_T$ photons and dileptons, baryon suppression and enhanced strange meson production. We propose to observe this newly predicted class of events at LHC and RHIC.

  12. Structural Basis for Ubiquitin Recognition by the Human ESCRT-II EAP45 GLUE Domain

    Energy Technology Data Exchange (ETDEWEB)

    Alam,S.; Langelier, C.; Whitby, F.; Koirala, S.; Robinson, H.; Hill, C.; Sundquist, W.

    2006-01-01

    ESCRT-IESCRT-IIGLUEEAP45VPS36The ESCRT-I and ESCRT-II complexes help sort ubiquitinated proteins into vesicles that accumulate within multivesicular bodies (MVBs). Crystallographic and biochemical analyses reveal that the GLUE domain of the human ESCRT-II EAP45 (also called VPS36) subunit is a split pleckstrin-homology domain that binds ubiquitin along one edge of the {beta}-sandwich. The structure suggests how human ESCRT-II can couple recognition of ubiquitinated cargoes and endosomal phospholipids during MVB protein sorting.

  13. TRANS-ARTERIAL EMBOLIZATION WITH N-BUTYL CYANOACRYLATE GLUE FOR RENAL BLEEDING: Case Report

    Directory of Open Access Journals (Sweden)

    Benny Young

    2014-08-01

    Full Text Available Background: The objectivity in management of renal bleeding is to preserve a significant renal parenchyma tissue and prevent associated morbidities like anemic shock or renal impairment from substantial nephron demise or obstructed uropathy. Trans-arterial embolization therapy by interventional radiology offers a high success rate with potential of reserving normal renal tissue. The selection material for renal arterial embolization largely depends on vasculature anatomy and end-point of procedure. N-butyl cyanoacrylate glue in our experience is applied in lesion supplied by small size of end-artery. 

  14. Uncertainty assessment of integrated distributed hydrological models using GLUE with Markov chain Monte Carlo sampling

    DEFF Research Database (Denmark)

    Blasone, Roberta-Serena; Madsen, Henrik; Rosbjerg, Dan

    2008-01-01

    uncertainty estimation (GLUE) procedure based on Markov chain Monte Carlo sampling is applied in order to improve the performance of the methodology in estimating parameters and posterior output distributions. The description of the spatial variations of the hydrological processes is accounted for by defining...... the identifiability of the parameters and results in satisfactory multi-variable simulations and uncertainty estimates. However, the parameter uncertainty alone cannot explain the total uncertainty at all the sites, due to limitations in the distributed data included in the model calibration. The study also indicates...

  15. Uncertainty and sensitivity assessments of an agricultural-hydrological model (RZWQM2) using the GLUE method

    Science.gov (United States)

    Sun, Mei; Zhang, Xiaolin; Huo, Zailin; Feng, Shaoyuan; Huang, Guanhua; Mao, Xiaomin

    2016-03-01

    Quantitatively ascertaining and analyzing the effects of model uncertainty on model reliability is a focal point for agricultural-hydrological models due to more uncertainties of inputs and processes. In this study, the generalized likelihood uncertainty estimation (GLUE) method with Latin hypercube sampling (LHS) was used to evaluate the uncertainty of the RZWQM-DSSAT (RZWQM2) model outputs responses and the sensitivity of 25 parameters related to soil properties, nutrient transport and crop genetics. To avoid the one-sided risk of model prediction caused by using a single calibration criterion, the combined likelihood (CL) function integrated information concerning water, nitrogen, and crop production was introduced in GLUE analysis for the predictions of the following four model output responses: the total amount of water content (T-SWC) and the nitrate nitrogen (T-NIT) within the 1-m soil profile, the seed yields of waxy maize (Y-Maize) and winter wheat (Y-Wheat). In the process of evaluating RZWQM2, measurements and meteorological data were obtained from a field experiment that involved a winter wheat and waxy maize crop rotation system conducted from 2003 to 2004 in southern Beijing. The calibration and validation results indicated that RZWQM2 model can be used to simulate the crop growth and water-nitrogen migration and transformation in wheat-maize crop rotation planting system. The results of uncertainty analysis using of GLUE method showed T-NIT was sensitive to parameters relative to nitrification coefficient, maize growth characteristics on seedling period, wheat vernalization period, and wheat photoperiod. Parameters on soil saturated hydraulic conductivity, nitrogen nitrification and denitrification, and urea hydrolysis played an important role in crop yield component. The prediction errors for RZWQM2 outputs with CL function were relatively lower and uniform compared with other likelihood functions composed of individual calibration criterion. This

  16. Nail Damage (Severe Onychodystrophy) Induced by Acrylate Glue: Scanning Electron Microscopy and Energy Dispersive X-Ray Investigations

    Science.gov (United States)

    Pinteala, Tudor; Chiriac, Anca Eduard; Rosca, Irina; Larese Filon, Francesca; Pinteala, Mariana; Chiriac, Anca; Podoleanu, Cristian; Stolnicu, Simona; Coros, Marius Florin; Coroaba, Adina

    2017-01-01

    Background Scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) techniques have been used in various fields of medical research, including different pathologies of the nails; however, no studies have focused on obtaining high-resolution microscopic images and elemental analysis of disorders caused by synthetic nails and acrylic adhesives. Methods Damaged/injured fingernails caused by the use of acrylate glue and synthetic nails were investigated using SEM and EDX methods. Results SEM and EDX proved that synthetic nails, acrylic glue, and nails damaged by contact with acrylate glue have a different morphology and different composition compared to healthy human nails. Conclusions SEM and EDX analysis can give useful information about the aspects of topography (surface sample), morphology (shape and size), hardness or reflectivity, and the elemental composition of nails. PMID:28232921

  17. Study of some optical glues for the Compact Muon Solenoid at the Large Hadron Collider of CERN

    CERN Document Server

    Montecchi, Marco

    2001-01-01

    Two Avalanche Photodiodes will measure the light produced in each of the 61,200 PbWO4 crystals composing the barrel part of the electromagnetic calorimeter of the Compact Muon Solenoid (CMS) at the Large Hadron Collider of CERN. To improve the collection of the photons, these detectors will be glued to the crystal. To be used in CMS, the optical glue must fulfil several requirements. The paper describes those requirements and reports the results of the investigation of several commercial optical glues. In particular, refractive index, absorption length, radiation hardness and forecast ageing after 15 years are reported. The most promising glue for CMS was more deeply investigated, in particular its chemical composition, chemical compatibility with the other parts of the calorimeter and curing time in realistic conditions.

  18. Lifetime Improvement of Organic Light Emitting Diodes using LiF Thin Film and UV Glue Encapsulation

    Science.gov (United States)

    Huang, Jian-Ji; Su, Yan-Kuin; Chang, Ming-Hua; Hsieh, Tsung-Eong; Huang, Bohr-Ran; Wang, Shun-Hsi; Chen, Wen-Ray; Tsai, Yu-Sheng; Hsieh, Huai-En; Liu, Mark O.; Juang, Fuh-Shyang

    2008-07-01

    This work demonstrates the use of lithium fluoride (LiF) as a passivation layer and a newly developed UV glue for encapsulation on the LiF passivation layer to enhance the stability of organic light-emitting devices (OLEDs). Devices with double protective layers showed a 25-fold increase in operational lifetime compared to those without any packaging layers. LiF has a low melting point and insulating characteristics and it can be adapted as both a protective layer and pre-encapsulation film. The newly developed UV glue has a fast curing time of only 6 s and can be directly spin-coated onto the surface of the LiF passivation layer. The LiF thin film plus spin-coated UV glue is a simple packaging method that reduces the fabrication costs of OLEDs.

  19. Alternative glues for the production of ATLAS silicon strip modules for the Phase-II upgrade of the ATLAS inner detector

    Energy Technology Data Exchange (ETDEWEB)

    Poley, Luise [DESY, Zeuthen (Germany); Humboldt Univ. Berlin (Germany); Bloch, Ingo [DESY, Zeuthen (Germany); Edwards, Sam [Birmingham Univ. (United Kingdom); and others

    2016-04-15

    The Phase-II upgrade of the ATLAS detector for the High Luminosity Large Hadron Collider (HL-LHC) includes the replacement of the current Inner Detector with an all-silicon tracker consisting of pixel and strip detectors. The current Phase-II detector layout requires the construction of 20,000 strip detector modules consisting of sensor, circuit boards and readout chips, which are connected mechanically using adhesives. The adhesive between readout chips and circuit board is a silver epoxy glue as was used in the current ATLAS SemiConductor Tracker (SCT). This glue has several disadvantages, which motivated the search for an alternative. This paper presents a study concerning the use of six ultra-violet (UV) cure glues and a glue pad for use in the assembly of silicon strip detector modules for the ATLAS upgrade. Trials were carried out to determine the ease of use, the thermal conduction and shear strength, thermal cycling, radiation hardness, corrosion resistance and shear strength tests. These investigations led to the exclusion of three UV cure glues as well as the glue pad. Three UV cure glues were found to be possible better alternatives. Results from electrical tests of first prototype modules constructed using these glues are presented.

  20. A split mouth randomized controlled study to evaluate the adjunctive effect of platelet-rich fibrin to coronally advanced flap in Miller′s class-I and II recession defects

    Directory of Open Access Journals (Sweden)

    Rajan Padma

    2013-01-01

    Full Text Available Background: There are various techniques developed to treat the exposed roots, a recent innovation in dentistry is the use of second generation platelet concentrate which is an autologous platelet-rich fibrin gel (PRF with growth factors and cicatricial properties for root coverage procedures. Therefore, the present research was undertaken to study the additional benefits of PRF when used along with coronally advanced flap (CAF. Materials and Methods: Total of 15 systemically healthy subjects presenting bilateral isolated Miller′s class I and II recession were enrolled into the study. Each patient was randomly treated with a combination of CAF along with a platelet-rich fibrin (PRF membrane on the test site and CAF alone on the control site. Recession depth, clinical attachment level (CAL, and width of keratinized gingiva (WKG were compared with baseline at 1, 3, and 6 months between test and control sites. Results: Mean percentage root coverage in the test group after 1, 3, and 6 months was 34.58, 70.73, and 100, respectively. Differences between the control and test groups were statistically significant. This study also showed a statistically significant increase in WKG in the test group (2.94 ± 0.77 at baseline to 5.38 ± 1.67 at 6 months. Conclusion: CAF is a predictable treatment for isolated Miller′s class I and II recession defects. The addition of PRF membrane with CAF provides superior root coverage with additional benefits of gain in CAL and WKG at 6 months postoperatively.

  1. Optimization of acidic fibroblast growth factor (FGF-1) and its delivery through a modified degradable fibrin scaffold

    Science.gov (United States)

    Pandit, Abhay Smashikant

    The aim of this investigation was to develop a degradable fibrin wound dressing that can deliver an optimized dose of acidic fibroblast growth factor (FGF-1). This aim led to three distinct phases of study. In the first phase, a structurally modified fibrin degradable scaffold was developed and tested in a rabbit ear ulcer model. A significant increase in the angiogenic and fibroblastic response with a corresponding decrease in healing time was seen in the modified fibrin-treated ulcers as compared with untreated ulcers and ulcers treated with non-modified fibrin systems. In the second phase of the study, a biochemical factor, FGF-1, was added to this scaffold. An optimal dose of 8 mug of FGF-1 was determined to be required to initiate a desired wound-healing response in a rabbit ear ulcer model, based on an enhanced angiogenic and fibroblastic response and an increased epithelialization rate. The objective of the last phase was to investigate the efficacy of a modified scaffold as a vehicle for FGF-1. In vivo testing was conducted in a full-thickness defect model in a rabbit. Improvements were seen in the angiogenic and fibroblastic responses in the FGF-1/modified fibrin treatment group and, hence, FGF-1/modified fibrin was the preferred treatment. In conclusion, the modified fibrin/FGF-1 matrix served as a suitable vehicle for the growth factor, providing a desired healing response and a desirable release rate and, thus, was determined to be an effective scaffold.

  2. Cell manipulation in autologous chondrocyte implantation: from research to cleanroom.

    Science.gov (United States)

    Roseti, Livia; Serra, Marta; Tigani, Domenico; Brognara, Irene; Lopriore, Annamaria; Bassi, Alessandra; Fornasari, Pier Maria

    2008-04-01

    In the field of orthopaedics, autologous chondrocyte implantation is a technique currently used for the regeneration of damaged articular cartilage. There is evidence of the neo-formation of tissue displaying characteristics similar to hyaline cartilage. In vitro chondrocyte manipulation is a crucial phase of this therapeutic treatment consisting of different steps: cell isolation from a cartilage biopsy, expansion in monolayer culture and growth onto a three-dimensional biomaterial to implant in the damaged area. To minimise the risk of in vitro cell contamination, the manipulation must be performed in a controlled environment such as a cleanroom. Moreover, the choice of reagents and raw material suitable for clinical use in humans and the translation of research protocols into standardised production processes are important. In this study we describe the preliminary results obtained by the development of chondrocyte manipulation protocols (isolation and monolayer expansion) in cleanrooms for the application of autologous implantation.

  3. Importance of mesenchymal stem cells in autologous fat grafting

    DEFF Research Database (Denmark)

    Trojahn Kølle, Stig-Frederik; Oliveri, Roberto S; Glovinski, Peter Viktor

    2012-01-01

    Autologous fat grafting (lipofilling) enables repair and augmentation of soft tissues and is increasingly used both in aesthetic and reconstructive surgery. Autologous fat has several advantages, including biocompatibility, versatility, natural appearance, and low donor site morbidity. The main...... the fat graft with adipose tissue-derived mesenchymal stem cells (ASC) before transplantation. We have reviewed original studies published on fat transplantation enriched with ASC. We found four murine and three human studies that investigated the subject after a sensitive search of publications...... limitation is unpredictable graft resorption, which ranges from 25%-80%, probably as a result of ischaemia and lack of neoangiogenesis. To obviate these disadvantages, several studies have searched for new ways of increasing the viability of the transplanted tissue. One promising approach has been to enrich...

  4. Safety and efficacy of a novel, dry powder fibrin sealant for hemostasis in hepatic resection.

    NARCIS (Netherlands)

    Ruitenbeek, K.; Ayez, N.; Verhoef, C.; Wilt, J.H.W. de; Bottema, J.; Rijken, A.M.; Rij, M. van; Koopman, J; Zuckerman, L.A.; Frohna, P.; Porte, R.J.

    2014-01-01

    BACKGROUND/AIMS: Fibrocaps is a dry powder fibrin sealant containing human plasma-derived fibrinogen and thrombin. The safety, efficacy, and application methods for Fibrocaps were evaluated in an exploratory, first-in-human, noncomparative, clinical study. METHODS: Patients with minor bleeding/oozin

  5. Safety and Efficacy of a Novel, Dry Powder Fibrin Sealant for Hemostasis in Hepatic Resection

    NARCIS (Netherlands)

    Ruitenbeek, Karin; Ayez, N.; Verhoef, C.; de Wilt, J. Hans W.; Bottema, Jan; Rijken, Arjen M.; van Rij, Monique; Koopman, Jaap; Zuckerman, Linda A.; Frohna, Paul; Porte, Robert J.

    2014-01-01

    Background/Aims: Fibrocaps is a dry powder fibrin sealant containing human plasma-derived fibrinogen and thrombin. The safety, efficacy, and application methods for Fibrocaps were evaluated in an exploratory, first-in-human, non-comparative, clinical study. Methods: Patients with minor bleeding/oozi

  6. The Effect of Synovial Fluid Enzymes on the Biodegradability of Collagen and Fibrin Clots

    Directory of Open Access Journals (Sweden)

    Martha M. Murray

    2011-08-01

    Full Text Available Recently there has been a great deal of interest in the use of biomaterials to stimulate wound healing. This is largely due to their ability to centralize high concentrations of compounds known to promote wound healing at a needed location. Joints present a unique challenge to using scaffolds because of the presence of enzymes in synovial fluid which are known to degrade materials that would be stable in other parts of the body. The hypothesis of this study was that atelocollagen scaffolds would have greater resistance to enzymatic degradation than scaffolds made of gelatin, fibrin and whole blood. To test this hypothesis, collagen and fibrin-based scaffolds were placed in matrix metallopeptidase-1 (MMP-1, elastase, and plasmin solutions at physiologic concentrations, and the degradation of each scaffold was measured at varying time points. The atelocollagen scaffolds had a significantly greater resistance to degradation by MMP-1, elastase and plasmin over the fibrin based scaffolds. The results suggest that atelocollagen-based scaffolds may provide some protection against premature degradation by synovial fluid enzymes over fibrin-based matrices.

  7. Lyophilized Platelet-Rich Fibrin (PRF Promotes Craniofacial Bone Regeneration through Runx2

    Directory of Open Access Journals (Sweden)

    Qi Li

    2014-05-01

    Full Text Available Freeze-drying is an effective means to control scaffold pore size and preserve its composition. The purpose of the present study was to determine the applicability of lyophilized Platelet-rich fibrin (LPRF as a scaffold for craniofacial tissue regeneration and to compare its biological effects with commonly used fresh Platelet-rich fibrin (PRF. LPRF caused a 4.8-fold ± 0.4-fold elevation in Runt-related transcription factor 2 (Runx2 expression in alveolar bone cells, compared to a 3.6-fold ± 0.2-fold increase when using fresh PRF, and a more than 10-fold rise of alkaline phosphatase levels and mineralization markers. LPRF-induced Runx2 expression only occurred in alveolar bone and not in periodontal or dental follicle cells. LPRF also caused a 1.6-fold increase in osteoblast proliferation (p < 0.001 when compared to fresh PRF. When applied in a rat craniofacial defect model for six weeks, LPRF resulted in 97% bony coverage of the defect, compared to 84% for fresh PRF, 64% for fibrin, and 16% without scaffold. Moreover, LPRF thickened the trabecular diameter by 25% when compared to fresh PRF and fibrin, and only LPRF and fresh PRF resulted in the formation of interconnected trabeculae across the defect. Together, these studies support the application of lyophilized PRF as a biomimetic scaffold for craniofacial bone regeneration and mineralized tissue engineering.

  8. Long-Standing Motor and Sensory Recovery following Acute Fibrin Sealant Based Neonatal Sciatic Nerve Repair

    Directory of Open Access Journals (Sweden)

    Natalia Perussi Biscola

    2016-01-01

    Full Text Available Brachial plexus lesion results in loss of motor and sensory function, being more harmful in the neonate. Therefore, this study evaluated neuroprotection and regeneration after neonatal peripheral nerve coaptation with fibrin sealant. Thus, P2 neonatal Lewis rats were divided into three groups: AX: sciatic nerve axotomy (SNA without treatment; AX+FS: SNA followed by end-to-end coaptation with fibrin sealant derived from snake venom; AX+CFS: SNA followed by end-to-end coaptation with commercial fibrin sealant. Results were analyzed 4, 8, and 12 weeks after lesion. Astrogliosis, microglial reaction, and synapse preservation were evaluated by immunohistochemistry. Neuronal survival, axonal regeneration, and ultrastructural changes at ventral spinal cord were also investigated. Sensory-motor recovery was behaviorally studied. Coaptation preserved synaptic covering on lesioned motoneurons and led to neuronal survival. Reactive gliosis and microglial reaction decreased in the same groups (AX+FS, AX+CFS at 4 weeks. Regarding axonal regeneration, coaptation allowed recovery of greater number of myelinated fibers, with improved morphometric parameters. Preservation of inhibitory synaptic terminals was accompanied by significant improvement in the motor as well as in the nociceptive recovery. Overall, the present data suggest that acute repair of neonatal peripheral nerves with fibrin sealant results in neuroprotection and regeneration of motor and sensory axons.

  9. Modelling of platelet-fibrin clot formation in flow with a DPD-PDE method.

    Science.gov (United States)

    Tosenberger, A; Ataullakhanov, F; Bessonov, N; Panteleev, M; Tokarev, A; Volpert, V

    2016-02-01

    The paper is devoted to mathematical modelling of clot growth in blood flow. Great complexity of the hemostatic system dictates the need of usage of the mathematical models to understand its functioning in the normal and especially in pathological situations. In this work we investigate the interaction of blood flow, platelet aggregation and plasma coagulation. We develop a hybrid DPD-PDE model where dissipative particle dynamics (DPD) is used to model plasma flow and platelets, while the regulatory network of plasma coagulation is described by a system of partial differential equations. Modelling results confirm the potency of the scenario of clot growth where at the first stage of clot formation platelets form an aggregate due to weak inter-platelet connections and then due to their activation. This enables the formation of the fibrin net in the centre of the platelet aggregate where the flow velocity is significantly reduced. The fibrin net reinforces the clot and allows its further growth. When the clot becomes sufficiently large, it stops growing due to the narrowed vessel and the increase of flow shear rate at the surface of the clot. Its outer part is detached by the flow revealing the inner part covered by fibrin. This fibrin cap does not allow new platelets to attach at the high shear rate, and the clot stops growing. Dependence of the final clot size on wall shear rate and on other parameters is studied.

  10. Supplementation of fibrin gels with sodium chloride enhances physical properties and ensuing osteogenic response.

    Science.gov (United States)

    Davis, H E; Miller, S L; Case, E M; Leach, J K

    2011-02-01

    Modifying the relative concentrations of fibrinogen and thrombin can control the physical properties of fibrin gels, while the viability of associated cells has been linked to the gel's final network structure. It was hypothesized that increasing the gel ionic strength during fabrication through supplementation with sodium chloride (NaCl) would provide an improved approach for tailoring the physical properties of fibrin gels and maintaining the viability and osteogenic potential of entrapped cells. Fibrin gels were formed by mixing fibrinogen, thrombin and calcium chloride with varying masses of NaCl (0-4.40% w/v), and the osteogenic potential of entrapped human mesenchymal stem cells (MSC) was examined over 14 days. Physical properties including gelation time, compressive modulus and fiber diameter were dependent upon NaCl content, with gels containing 2.60% NaCl possessing compressive moduli threefold higher than gels without NaCl. Alkaline phosphatase activity was highest for MSC entrapped in gels containing 2.15-2.60% NaCl after 14 days, and all gels exhibited increased calcium incorporation over the culture period. These data confirm that varying the salt concentration of the pre-gel solution can modulate the material properties of fibrin constructs without additional fibrinogen or thrombin, thereby offering a new approach for generating improved cell transplantation vehicles for use in bone tissue regeneration.

  11. Impaired thrombin generation and fibrin clot formation in patients with dilutional coagulopathy during major surgery.

    NARCIS (Netherlands)

    Schols, S.E.; Lance, M.D.; Feijge, M.A.; Damoiseaux, J.; Marcus, M.A.; Hamulyak, K.; Cate, H. ten; Heemskerk, J.W.M.; Pampus, E.C.M. van

    2010-01-01

    Patients subjected to haemodilution during surgery are at increased risk of bleeding. We hypothesised that, in the acquired dilutional coagulopathy, insufficient haemostasis is due to either insufficient thrombin generation or insufficient fibrin clot formation. In tissue factor-activated plasmas fr

  12. Platelet-Rich Fibrin Promotes Periodontal Regeneration and Enhances Alveolar Bone Augmentation

    Directory of Open Access Journals (Sweden)

    Qi Li

    2013-01-01

    Full Text Available In the present study we have determined the suitability of platelet-rich fibrin (PRF as a complex scaffold for periodontal tissue regeneration. Replacing PRF with its major component fibrin increased mineralization in alveolar bone progenitors when compared to periodontal progenitors, suggesting that fibrin played a substantial role in PRF-induced osteogenic lineage differentiation. Moreover, there was a 3.6-fold increase in the early osteoblast transcription factor RUNX2 and a 3.1-fold reduction of the mineralization inhibitor MGP as a result of PRF application in alveolar bone progenitors, a trend not observed in periodontal progenitors. Subcutaneous implantation studies revealed that PRF readily integrated with surrounding tissues and was partially replaced with collagen fibers 2 weeks after implantation. Finally, clinical pilot studies in human patients documented an approximately 5 mm elevation of alveolar bone height in tandem with oral mucosal wound healing. Together, these studies suggest that PRF enhances osteogenic lineage differentiation of alveolar bone progenitors more than of periodontal progenitors by augmenting osteoblast differentiation, RUNX2 expression, and mineralized nodule formation via its principal component fibrin. They also document that PRF functions as a complex regenerative scaffold promoting both tissue-specific alveolar bone augmentation and surrounding periodontal soft tissue regeneration via progenitor-specific mechanisms.

  13. Platelet-rich fibrin matrix improves wound angiogenesis via inducing endothelial cell proliferation.

    Science.gov (United States)

    Roy, Sashwati; Driggs, Jason; Elgharably, Haytham; Biswas, Sabyasachi; Findley, Muna; Khanna, Savita; Gnyawali, Urmila; Bergdall, Valerie K; Sen, Chandan K

    2011-11-01

    The economic, social, and public health burden of chronic ulcers and other compromised wounds is enormous and rapidly increasing with the aging population. The growth factors derived from platelets play an important role in tissue remodeling including neovascularization. Platelet-rich plasma (PRP) has been utilized and studied for the last four decades. Platelet gel and fibrin sealant, derived from PRP mixed with thrombin and calcium chloride, have been exogenously applied to tissues to promote wound healing, bone growth, hemostasis, and tissue sealing. In this study, we first characterized recovery and viability of as well as growth factor release from platelets in a novel preparation of platelet gel and fibrin matrix, namely platelet-rich fibrin matrix (PRFM). Next, the effect of PRFM application in a delayed model of ischemic wound angiogenesis was investigated. The study, for the first time, shows the kinetics of the viability of platelet-embedded fibrin matrix. A slow and steady release of growth factors from PRFM was observed. The vascular endothelial growth factor released from PRFM was primarily responsible for endothelial mitogenic response via extracellular signal-regulated protein kinase activation pathway. Finally, this preparation of PRFM effectively induced endothelial cell proliferation and improved wound angiogenesis in chronic wounds, providing evidence of probable mechanisms of action of PRFM in healing of chronic ulcers.

  14. Platelet-rich fibrin promotes periodontal regeneration and enhances alveolar bone augmentation.

    Science.gov (United States)

    Li, Qi; Pan, Shuang; Dangaria, Smit J; Gopinathan, Gokul; Kolokythas, Antonia; Chu, Shunli; Geng, Yajun; Zhou, Yanmin; Luan, Xianghong

    2013-01-01

    In the present study we have determined the suitability of platelet-rich fibrin (PRF) as a complex scaffold for periodontal tissue regeneration. Replacing PRF with its major component fibrin increased mineralization in alveolar bone progenitors when compared to periodontal progenitors, suggesting that fibrin played a substantial role in PRF-induced osteogenic lineage differentiation. Moreover, there was a 3.6-fold increase in the early osteoblast transcription factor RUNX2 and a 3.1-fold reduction of the mineralization inhibitor MGP as a result of PRF application in alveolar bone progenitors, a trend not observed in periodontal progenitors. Subcutaneous implantation studies revealed that PRF readily integrated with surrounding tissues and was partially replaced with collagen fibers 2 weeks after implantation. Finally, clinical pilot studies in human patients documented an approximately 5 mm elevation of alveolar bone height in tandem with oral mucosal wound healing. Together, these studies suggest that PRF enhances osteogenic lineage differentiation of alveolar bone progenitors more than of periodontal progenitors by augmenting osteoblast differentiation, RUNX2 expression, and mineralized nodule formation via its principal component fibrin. They also document that PRF functions as a complex regenerative scaffold promoting both tissue-specific alveolar bone augmentation and surrounding periodontal soft tissue regeneration via progenitor-specific mechanisms.

  15. Platelet-rich fibrin membranes as scaffolds for periosteal tissue engineering.

    NARCIS (Netherlands)

    Gassling, V.; Douglas, T.E.L.; Warnke, P.H.; Acil, Y.; Wiltfang, J.; Becker, S.T.

    2010-01-01

    OBJECTIVES: Platelet-rich fibrin (PRF)-based membranes have been used for covering alveolar ridge augmentation side in several in vivo studies. Few in vitro studies on PRF and no studies using human periosteal cells for tissue engineering have been published. The aim is a comparison of PRF with the

  16. Bioreactor Conditioning for Accelerated Remodeling of Fibrin-Based Tissue Engineered Heart Valves

    Science.gov (United States)

    Schmidt, Jillian Beth

    Fibrin is a promising scaffold material for tissue engineered heart valves, as it is completely biological, allows for engineered matrix alignment, and is able to be degraded and replaced with collagen by entrapped cells. However, the initial fibrin matrix is mechanically weak, and extensive in vitro culture is required to create valves with sufficient mechanical strength and stiffness for in vivo function. Culture in bioreactor systems, which provide cyclic stretching and enhance nutrient transport, has been shown to increase collagen production by cells entrapped in a fibrin scaffold, accelerating strengthening of the tissue and reducing the required culture time. In the present work, steps were taken to improve bioreactor culture conditions with the goal of accelerating collagen production in fibrin-based tissue engineered heart valves using two approaches: (i) optimizing the cyclic stretching protocol and (ii) developing a novel bioreactor system that permits transmural and lumenal flow of culture medium for improved nutrient transport. The results indicated that incrementally increasing strain amplitude cyclic stretching with small, frequent increments in strain amplitude was optimal for collagen production in our system. In addition, proof of concept studies were performed in the novel bioreactor system and increased cellularity and collagen deposition near the lumenal surface of the tissue were observed.

  17. Canine articular cartilage regeneration using mesenchymal stem cells seeded on platelet rich fibrin

    Science.gov (United States)

    Shams Asenjan, K.; Dehdilani, N.; Parsa, H.

    2017-01-01

    Objectives Mesenchymal stem cells have the ability to differentiate into various cell types, and thus have emerged as promising alternatives to chondrocytes in cell-based cartilage repair methods. The aim of this experimental study was to investigate the effect of bone marrow derived mesenchymal stem cells combined with platelet rich fibrin on osteochondral defect repair and articular cartilage regeneration in a canine model. Methods Osteochondral defects were created on the medial femoral condyles of 12 adult male mixed breed dogs. They were either treated with stem cells seeded on platelet rich fibrin or left empty. Macroscopic and histological evaluation of the repair tissue was conducted after four, 16 and 24 weeks using the International Cartilage Repair Society macroscopic and the O’Driscoll histological grading systems. Results were reported as mean and standard deviation (sd) and compared at different time points between the two groups using the Mann-Whitney U test, with a value regeneration. It is postulated that platelet rich fibrin creates a suitable environment for proliferation and differentiation of stem cells by releasing endogenous growth factors resulting in creation of a hyaline-like reparative tissue. Cite this article: D. Kazemi, K. Shams Asenjan, N. Dehdilani, H. Parsa. Canine articular cartilage regeneration using mesenchymal stem cells seeded on platelet rich fibrin: Macroscopic and histological assessments. Bone Joint Res 2017;6:98–107. DOI: 10.1302/2046-3758.62.BJR-2016-0188.R1. PMID:28235767

  18. Effect of implanting fibrin sealant with ropivacaine on pain after laparoscopic cholecystectomy

    Institute of Scientific and Technical Information of China (English)

    Jian-Zhu Fu; Jie Li; Ze-Li Yu

    2009-01-01

    AIM: To investigate the safety and efficacy of implanting fibrin sealant with sustained-release ropivacaine in the gallbladder bed for pain after laparoscopic cholecystectomy (LC). METHODS: Sixty patients (American Society of Anesthesiologists physical status was Ⅰ or Ⅱ and underwent LC) were randomly divided into three equal groups: group A (implantation of fibrin sealant in the gallbladder bed), group B (implantation of fibrin sealant carrying ropivacaine in the gallbladder bed), and group C (normal saline in the gallbladder bed). Postoperative pain was evaluated, and pain relief was assessed by visual analog scale (VAS) scoring. RESULTS: The findings showed that 81.7% of patients had visceral pain, 50% experienced parietal, and 26.7% reported shoulder pain after LC. Visceral pain was significantly less in group B patients than in the other groups ( P < 0.05), and only one patient in this group experienced shoulder pain. The mean VAS score in group B patients was lower than that in the other groups. CONCLUSION: Visceral pain is prominent after LC and can be effectively controlled by implanting fibrin sealant combined with ropivacaine in the gallbladder bed.

  19. Fibrin matrices enhance the transplant and efficacy of cytotoxic stem cell therapy for post-surgical cancer.

    Science.gov (United States)

    Bagó, Juli R; Pegna, Guillaume J; Okolie, Onyi; Hingtgen, Shawn D

    2016-04-01

    Tumor-homing cytotoxic stem cell (SC) therapy is a promising new approach for treating the incurable brain cancer glioblastoma (GBM). However, problems of retaining cytotoxic SCs within the post-surgical GBM resection cavity are likely to significantly limit the clinical utility of this strategy. Here, we describe a new fibrin-based transplant approach capable of increasing cytotoxic SC retention and persistence within the resection cavity, yet remaining permissive to tumoritropic migration. This fibrin-based transplant can effectively treat both solid and post-surgical human GBM in mice. Using our murine model of image-guided model of GBM resection, we discovered that suspending human mesenchymal stem cells (hMSCS) in a fibrin matrix increased initial retention in the surgical resection cavity 2-fold and prolonged persistence in the cavity 3-fold compared to conventional delivery strategies. Time-lapse motion analysis revealed that cytotoxic hMSCs in the fibrin matrix remain tumoritropic, rapidly migrating from the fibrin matrix to co-localize with cultured human GBM cells. We encapsulated hMSCs releasing the cytotoxic agent TRAIL (hMSC-sTR) in fibrin, and found hMSC-sTR/fibrin therapy reduced the viability of multiple 3-D human GBM spheroids and regressed established human GBM xenografts 3-fold in 11 days. Mimicking clinical therapy of surgically resected GBM, intra-cavity seeding of therapeutic hMSC-sTR encapsulated in fibrin reduced post-surgical GBM volumes 6-fold, increased time to recurrence 4-fold, and prolonged median survival from 15 to 36 days compared to control-treated animals. Fibrin-based SC therapy could represent a clinically compatible, viable treatment to suppress recurrence of post-surgical GBM and other lethal cancer types.

  20. Fibrin-mediated integrin signaling plays a critical role in hepatic regeneration after partial hepatectomy in mice

    Science.gov (United States)

    Beier, Juliane I.; Guo, Luping; Joshi-Barve, Swati; Ritzenthaler, Jeffrey D.; Roman, Jesse; Arteel, Gavin E.

    2016-01-01

    Background The regenerative capacity of the liver is critical for proper responses to injury. Fibrin extracellular matrix (ECM) deposition is a common response to insult and contributes to inflammatory liver injury. However, the role of this matrix in hepatic regeneration has not been determined. Objective The purpose of this study was first to determine the role of fibrin ECM in hepatic regeneration followed by the role of the fibrin-binding αvβ3 integrin in mediating this effect. Material and methods C57BI/6J (WT) or PAI-1 knockout (KO) mice underwent 70% partial hepatectomy (PHx); plasma and histologic indices of regeneration were determined, as well as expression of key genes involved in hepatic regeneration. Results PHx promoted transient fibrin deposition by activating coagulation and concomitantly decreasing fibrinolysis. Inhibiting fibrin deposition, either by blocking thrombin (hirudin) in WT mice or by knocking out PAI-1, was associated with a decrease in hepatocyte proliferation after PHx. This strongly suggested a role for fibrin ECM in liver regeneration. To investigate if αvβ3 integrin mediates this action, we tested the effects of the anti-αvβ3 cyclic peptide RGDfV in animals after PHx. As was observed with inhibition of fibrin deposition, competitive inhibition of αvβ3 integrin delayed regeneration after PHx, while not affecting fibrin deposition. These effects of RGDfV correlated with impaired angiogenesis and STAT3 signaling, as well as transient endothelial dysfunction. In conclusion, these data suggest that αvβ3 integrin plays an important role in coordinating hepatocyte division during liver regeneration after PHx via crosstalk with fibrin ECM. PMID:27493116

  1. AUTOLOGOUS SERUM SKIN TESTING (ASST) IN CHRONIC IDIOPATHIC URTICARIA

    OpenAIRE

    Arun; Suresh; Raghavendra; Vijay; Ramesh,; Asha; Manali; Jitendra

    2014-01-01

    Chronic idiopathic urticaria (CIU) is a form of urticaria , in which there appears to be persistent activation of mast cells , but the mechanism of mast cell triggering is unknown. The Autologous serum skin test (ASST) is an in vivo test which assesses auto reactivity. ASST could be good screening test for Autoreactive urticaria a subset of chronic idiopathic urticaria. AIMS : To study the clinical profile of chronic idiopathic urticaria and pattern of A...

  2. [4 years experience with the Ulm autologous transfusion concept].

    Science.gov (United States)

    Mehrkens, H H; Geiger, P; Schleinzer, W; Weindler, M; Wollinsky, K H; Pohland, H

    1990-04-01

    After a 4-year period in clinical practice the autologous transfusion concept Ulm (ATU) has proved its value. The effort and expense involved are entirely justified by obvious medical advantages. The patients' active involvement in the therapeutic procedure is a remarkable aspect of positive motivation. Furthermore, the medical staff is positively motivated, too, in spite of the obvious additional load to their daily routine work.

  3. Autologous Fat Transfer in a Patient with Lupus Erythematosus Profundus

    Directory of Open Access Journals (Sweden)

    Jimi Yoon

    2012-10-01

    Full Text Available Lupus erythematosus profundus, a form of chronic cutaneous lupus erythematosus, is a rare inflammatory disease involving in the lower dermis and subcutaneous tissues. It primarily affects the head, proximal upper arms, trunk, thighs, and presents as firm nodules, 1 to 3 cm in diameter. The overlying skin often becomes attached to the subcutaneous nodules and is drawn inward to produce deep, saucerized depressions. We present a rare case of lupus erythematosus profundus treated with autologous fat transfer.

  4. Local Therapy of Gonarthrosis Using Autologous Platelet-Enriched Plasma

    OpenAIRE

    2012-01-01

    The aim of the investigation is to assess clinical effect of local (intra-articular) application of autologous platelet-enriched plasma (PEP) in treating gonarthrosis. Materials and Methods. Clinical observation of 83 women with gonarthrosis was performed within 3 months. Within 3 weeks the patients were receiving PEP intra-articular injections of 5 ml twice a week. Results. In intra-articular PEP administration in patients with gonarthrosis, the change of knee joint functional state ...

  5. The effects of autologous platelet gel on wound healing.

    Science.gov (United States)

    Henderson, Jenifer L; Cupp, Craig L; Ross, E Victor; Shick, Paul C; Keefe, Michael A; Wester, Derin C; Hannon, Timothy; McConnell, Devin

    2003-08-01

    Laser resurfacing techniques have become a popular means of achieving rejuvenation of damaged skin. Interest is great in attempting to speed re-epithelialization and healing so that patients can return to their normal activities as quickly as possible. Previous studies have demonstrated that wounds heal more quickly when they are covered and kept moist than when they are left open to the air. Until now, no study has been conducted to investigate whether the healing process of a superficial skin burn might be accelerated by the use of an autologous platelet gel as a biologic dressing. Our study of five pigs showed that autologous platelet gel can influence wound healing by stimulating an intense inflammatory process that leads to highly significant increases in the production of extracellular matrices and granulation tissue. The platelet gel accelerated vascular ingrowth, increased fibroblastic proliferation, and accelerated collagen production. However, the gel did not appear to accelerate re-epithelialization. The aggressive production of granulation tissue and the acceleration of collagen production might mean that autologous platelet gel will have a future role in the treatment of burns because the highly vascularized bed it helps create should promote the success of skin grafting in patients with deep partial-thickness and full-thickness burns.

  6. Platelet-rich fibrin-induced bone marrow mesenchymal stem cell differentiation into osteoblast-like cells and neural cells

    Institute of Scientific and Technical Information of China (English)

    Qi Li; Yajun Geng; Lei Lu; Tingting Yang; Mingrui Zhang; Yanmin Zhou

    2011-01-01

    Bone marrow mesenchymal stem cells were allowed to develop for 14 days in a platelet-rich fibrin environment. Results demonstrated that platelet-rich fibrin significantly promoted bone marrow mesenchymal stem cell proliferation. In addition, there was a dose-dependent increase in Runt-related transcription factor-2 and bone morphogenetic protein-2 mRNA expression, as well as neuron-specific enolase and glial acidic protein. Results showed that platelet-rich fibrin promoted bone marrow mesenchymal stem cell proliferation and differentiation of osteoblastlike cells and neural cells in a dose-dependent manner.

  7. Immunohistochemical evaluation of tissue factor, fibrin/fibrinogen and D-dimers in canine gliomas.

    Science.gov (United States)

    de la Fuente, Cristian; Pumarola, Martí; Blasco, Ester; Fernández, Francisco; Viu, Judit; Añor, Sònia

    2014-06-01

    In human gliomas, tissue factor (TF) is overexpressed, associated with the grade of malignancy and influences tumour biology. Intra-tumoural fibrin/fibrinogen deposition and activation of the fibrinolytic system also play a role in tumour cell proliferation and angiogenesis. The first aim of the present study was to investigate TF expression and the presence of fibrin/fibrinogen and D-dimers in canine glioma biopsies, graded according to the World Health Organization (WHO) classification of tumours of the central nervous system. The second aim was to investigate the occurrence of intravascular thrombosis (IVT) in canine gliomas, as a potential histological marker of glioma type or grade of malignancy. An immunohistochemical study using antibodies against TF, fibrin/fibrinogen and D-dimers was performed with 24 glioma samples, including 15 oligodendrogliomas, 6 astrocytomas and 3 mixed gliomas. Immunohistochemical data were statistically analysed to determine whether there was any relationship between glioma type and grade of malignancy. All gliomas were moderate to strongly positive for TF and the staining score was significantly higher (P = 0.04) in high-grade (III or IV) than in low-grade (II) gliomas. Intra-tumoural fibrin/fibrinogen deposition was detected in all tumour biopsies assessed, and D-dimers were detected in 17/24 gliomas. IVT was a frequent finding, but was not linked to a specific glioma type or malignancy grade. TF expression, fibrin/fibrinogen deposition, extravascular fibrinolytic system activation and IVT occur in canine gliomas. Canine glioma might be a suitable model for studying coagulation and fibrinolysis as potential therapeutic targets for human gliomas.

  8. Inorganic Glue Enabling High Performance of Silicon Particles as Lithium Ion Battery Anode

    KAUST Repository

    Cui, Li-Feng

    2011-01-01

    Silicon, as an alloy-type anode material, has recently attracted lots of attention because of its highest known Li+ storage capacity (4200 mAh/g). But lithium insertion into and extraction from silicon are accompanied by a huge volume change, up to 300, which induces a strong strain on silicon and causes pulverization and rapid capacity fading due to the loss of the electrical contact between part of silicon and current collector. Silicon nanostructures such as nanowires and nanotubes can overcome the pulverization problem, however these nano-engineered silicon anodes usually involve very expensive processes and have difficulty being applied in commercial lithium ion batteries. In this study, we report a novel method using amorphous silicon as inorganic glue replacing conventional polymer binder. This inorganic glue method can solve the loss of contact issue in conventional silicon particle anode and enables successful cycling of various sizes of silicon particles, both nano-particles and micron particles. With a limited capacity of 800 mAh/g, relatively large silicon micron-particles can be stably cycled over 200 cycles. The very cheap production of these silicon particle anodes makes our method promising and competitive in lithium ion battery industry. © 2011 The Electrochemical Society.

  9. Equifinality of formal (DREAM) and informal (GLUE) bayesian approaches in hydrologic modeling?

    Energy Technology Data Exchange (ETDEWEB)

    Vrugt, Jasper A [Los Alamos National Laboratory; Robinson, Bruce A [Los Alamos National Laboratory; Ter Braak, Cajo J F [NON LANL; Gupta, Hoshin V [NON LANL

    2008-01-01

    In recent years, a strong debate has emerged in the hydrologic literature regarding what constitutes an appropriate framework for uncertainty estimation. Particularly, there is strong disagreement whether an uncertainty framework should have its roots within a proper statistical (Bayesian) context, or whether such a framework should be based on a different philosophy and implement informal measures and weaker inference to summarize parameter and predictive distributions. In this paper, we compare a formal Bayesian approach using Markov Chain Monte Carlo (MCMC) with generalized likelihood uncertainty estimation (GLUE) for assessing uncertainty in conceptual watershed modeling. Our formal Bayesian approach is implemented using the recently developed differential evolution adaptive metropolis (DREAM) MCMC scheme with a likelihood function that explicitly considers model structural, input and parameter uncertainty. Our results demonstrate that DREAM and GLUE can generate very similar estimates of total streamflow uncertainty. This suggests that formal and informal Bayesian approaches have more common ground than the hydrologic literature and ongoing debate might suggest. The main advantage of formal approaches is, however, that they attempt to disentangle the effect of forcing, parameter and model structural error on total predictive uncertainty. This is key to improving hydrologic theory and to better understand and predict the flow of water through catchments.

  10. Beam asymmetry Σ for π0 and η photoproduction on the proton at GlueX

    Science.gov (United States)

    Zhang, Zhenyu; GlueX Collaboration

    2016-09-01

    Measurements of meson photoproduction at high energies began almost 50 years ago with bubble chamber experiments at SLAC, DESY, and Cambridge. These data have been successfully described through Regge theory in terms of t-channel quasi-particle exchange. High statistics measurements of pseudoscalar meson photoproduction at GlueX, using the 9 GeV linearly-polarized, tagged photon beam in Jefferson Lab's Hall D, will provide important new constraints on these Regge models. These measurements will test our understanding of the photoproduction mechanism at high energy, which is a necessary first step toward the broader meson spectroscopy program at GlueX. Through finite energy sum rules, these measurements can also impose new constraints on the extraction of nucleon resonances from low energy photoproduction data. In this talk, preliminary results for the linearly polarized photon beam asymmetry Σ for the exclusive reactions γp -> pπ0 and γp -> pη will be presented. These are the first measurements of the η beam asymmetry at these energies. Supported by Jefferson Science Associates, LLC under U.S. DOE Contract No. DE-AC05-06OR23177 and the China Scholarship Council No. 201506275156.

  11. Study of the nature of the confinement in the GlueX experiment

    Energy Technology Data Exchange (ETDEWEB)

    Somov, S. [Moscow Engineering Physics Institute; Berdnikov, Vladmir [Moscow Engineering Physics Institute; Tolstukhin, Ivan [Moscow Engineering Physics Institute; Somov, Alexander S. [Jefferson Lab, Newport News, VA

    2015-11-01

    Confinement is a fundamental property of quantum chromodynamics (QCD) associated with the unique role of the gluonic field responsible for binding quarks in hadrons. Understanding the role of gluons in the confinement of quarks is one of the most tantalizing topics in modern particle physics to be explored. The new experiment GlueX has been recently constructed at Jefferson Lab. The experiment was designed to search for hybrid mesons with exotic quantum numbers using a beam of linearly polarized photons incident on a liquid hydrogen target. The spectrum of these states and their mass splitting from normal mesons may yield information on confinement. In addition, these observations in combination with detailed chromodynamics calculations such as on the Lattice can provide important tests for our understanding of the role of gluons. The production of exotic mesons is expected to be enhanced in p interactions, where the experimental data is very limited. We present the description of the GlueX detector, beam line, and first results of the commissioning with photon beam.

  12. Species identification of Asini Corii Collas (donkey glue) by PCR amplification of cytochrome b gene.

    Science.gov (United States)

    Kumeta, Yukie; Maruyama, Takuro; Asama, Hiroshi; Yamamoto, Yutaka; Hakamatsuka, Takashi; Goda, Yukihiro

    2014-01-01

    Asini Corii Collas (ACC; donkey glue) is a crude drug used to promote hematopoiesis and arrest bleeding. Because adulteration of the drug with substances from other animals such as horses, cattle, and pigs has been found, we examined PCR methods based on the sequence of the cytochrome b gene for source species identification. Two strategies for extracting DNA from ACC were compared, and the ion-exchange resin procedure was revealed to be more suitable than the silica-based one. Using DNA extracted from ACC by the ion-exchange resin procedure, PCR methods for species-specific detection of donkey, horse, cattle, and pig substances were established. When these species-specific PCR methods were applied to ACC, amplicons were obtained only by the donkey-specific PCR. Cattle-specific PCR detected as little as 0.1% admixture of cattle glue in the ACC. These results suggest that the species-specific PCR methods established in this study would be useful for simple and easy detection of adulteration of ACC.

  13. Membranous nephropathy in autologous hematopoietic stem cell transplant: autologous graft-versus-host disease or autoimmunity induction?

    Science.gov (United States)

    Abudayyeh, Ala; Truong, Luan D.; Beck, Laurence H.; Weber, Donna M.; Rezvani, Katy; Abdelrahim, Maen

    2015-01-01

    With the increasing utility of hematopoietic stem cell transplantation (SCT) as a treatment for cancer and noncancerous disorders, more challenges and complications associated with SCT have emerged. Renal injury immediately after transplant is common and well understood, but long-term renal injury is becoming more evident. Chronic graft-versus-host disease (GVHD) is a known long-term complication of SCT, and membranous nephropathy (MN) is emerging as the most common cause of SCT-associated glomerular pathology. In this case report, we present a patient who developed features of anti-PLA2R antibody-negative MN following autologous SCT. The renal injury responded well to steroids and further response to rituximab therapy was noted, suggesting antibody-mediated autoimmune glomerular disease. We also present a review of the literature on autologous GVHD and the role of T and B cells in induction of autoimmunity by SCT. PMID:26251713

  14. Transplantation of Reprogrammed Autologous Stem Cells for Chronic Pain and Drug Abuse

    Science.gov (United States)

    2015-10-01

    AWARD NUMBER: W81XWH-11-1-0673 TITLE: Transplantation of Reprogrammed Autologous Stem Cells for Chronic Pain and Drug Abuse PRINCIPAL...CONTRACT NUMBER Transplantation of Reprogrammed Autologous Stem Cells for Chronic Pain and Drug Abuse 5b. GRANT NUMBER: W81XWH-11-1-0673 5c. PROGRAM...Tolerance, Drug abuse , Cell cultures, Spinal transplantation of autologous stem cells, Animal behavioral tests 16. SECURITY CLASSIFICATION OF: 17

  15. Regeneration of Cartilage in Human Knee Osteoarthritis with Autologous Adipose Tissue-Derived Stem Cells and Autologous Extracellular Matrix

    Directory of Open Access Journals (Sweden)

    Jaewoo Pak

    2016-08-01

    Full Text Available This clinical case series demonstrates that percutaneous injections of autologous adipose tissue-derived stem cells (ADSCs and homogenized extracellular matrix (ECM in the form of adipose stromal vascular fraction (SVF, along with hyaluronic acid (HA and platelet-rich plasma (PRP activated by calcium chloride, could regenerate cartilage-like tissue in human knee osteoarthritis (OA patients. Autologous lipoaspirates were obtained from adipose tissue of the abdominal origin. Afterward, the lipoaspirates were minced to homogenize the ECM. These homogenized lipoaspirates were then mixed with collagenase and incubated. The resulting mixture of ADSCs and ECM in the form of SVF was injected, along with HA and PRP activated by calcium chloride, into knees of three Korean patients with OA. The same affected knees were reinjected weekly with additional PRP activated by calcium chloride for 3 weeks. Pretreatment and post-treatment magnetic resonance imaging (MRI data, functional rating index, range of motion (ROM, and pain score data were then analyzed. All patients' MRI data showed cartilage-like tissue regeneration. Along with MRI evidence, the measured physical therapy outcomes in terms of ROM, subjective pain, and functional status were all improved. This study demonstrates that percutaneous injection of ADSCs with ECM contained in autologous adipose SVF, in conjunction with HA and PRP activated by calcium chloride, is a safe and potentially effective minimally invasive therapy for OA of human knees.

  16. Use of autologous bone marrow mononuclear cells and cultured bone marrow stromal cells in dogs with orthopaedic lesions.

    Science.gov (United States)

    Crovace, A; Favia, A; Lacitignola, L; Di Comite, M S; Staffieri, F; Francioso, E

    2008-09-01

    The aim of the study is to evaluate the clinical application in veterinary orthopedics of bone marrow mononuclear cells (BMMNCs) and cultured bone marrow stromal cells (cBMSCs) for the treatment of some orthopaedic lesions in the dog. The authors carried out a clinical study on 14 dogs of different breed, age and size with the following lesions: 1 bone cyst of the glenoid rime; 2 nonunion of the tibia; 3 nonunion of the femur; 2 lengthening of the radius; 1 large bone defect of the distal radius;1 nonunion with carpus valgus; 4 Legg-Calvé-Perthés disease. In 9 cases the BMMCNs were used in combination with a three dimensional resorbable osteogenic scaffold the chemical composition and size of which facilitates the ingrowth of bone. In these cases the BMMNCs were suspended in an adequate amount of fibrin glue and then distribuited uniformly on a Tricalcium-Phosphate (TCP) scaffold onto which were also added some drops of thrombin. In 1 case of nonunion of the tibia and in 3 cases of Legg-Calvè-Perthés (LCP) disease the cultured BMSCs were used instead because of the small size of the dogs and of the little amount of aspirated bone marrow. X-ray examinations were performed immediately after the surgery. Clinical, ultrasounds and X-ray examinations were performed after 20 days and then every month. Until now the treated dogs have shown very good clinical and X-ray results. One of the objectives of the study was to use the BMMNCs in clinical application in orthopaedic lesions in the dog. The advantages of using the cells immediately after the bone marrow is collected, are that the surgery can be performed the same day, the cells do not need to be expanded in vitro, they preserve their osteogenic potential to form bone and promote the proper integration of the implant with the bone and lastly, the technique is easier and the costs are lower.

  17. Stochastic capture zone analysis of an arsenic-contaminated well using the generalized likelihood uncertainty estimator (GLUE) methodology

    Science.gov (United States)

    Morse, Brad S.; Pohll, Greg; Huntington, Justin; Rodriguez Castillo, Ramiro

    2003-06-01

    In 1992, Mexican researchers discovered concentrations of arsenic in excess of World Heath Organization (WHO) standards in several municipal wells in the Zimapan Valley of Mexico. This study describes a method to delineate a capture zone for one of the most highly contaminated wells to aid in future well siting. A stochastic approach was used to model the capture zone because of the high level of uncertainty in several input parameters. Two stochastic techniques were performed and compared: "standard" Monte Carlo analysis and the generalized likelihood uncertainty estimator (GLUE) methodology. The GLUE procedure differs from standard Monte Carlo analysis in that it incorporates a goodness of fit (termed a likelihood measure) in evaluating the model. This allows for more information (in this case, head data) to be used in the uncertainty analysis, resulting in smaller prediction uncertainty. Two likelihood measures are tested in this study to determine which are in better agreement with the observed heads. While the standard Monte Carlo approach does not aid in parameter estimation, the GLUE methodology indicates best fit models when hydraulic conductivity is approximately 10-6.5 m/s, with vertically isotropic conditions and large quantities of interbasin flow entering the basin. Probabilistic isochrones (capture zone boundaries) are then presented, and as predicted, the GLUE-derived capture zones are significantly smaller in area than those from the standard Monte Carlo approach.

  18. GlueX at Jefferson Lab: a search for exotic states of matter in photon-proton collisions

    Energy Technology Data Exchange (ETDEWEB)

    Shepherd, Matt [Cornell University, Ithaca, NY

    2014-11-01

    The GlueX Experiment, which is currently under construction as a component of the 12 GeV upgrade to Jefferson Lab, will utilize photoproduction on a proton target to search for hybrid mesons in the light quark sector. Recent first-principles calculations of the hadron spectrum in Quantum Chromodynamics suggest the presence of bound states in the meson spectrum that cannot arise from a quark and an anti-quark. Such states appear to have valance gluonic content or gluonic degrees of freedom and are called hybrid mesons. An interesting subset of these, the “exotic hybrid mesons," have total angular momentum, parity, and charge conjugation quantum numbers that cannot be formed with a pair of spin-1/2 fermions. By performing an amplitude analysis of photon-proton reactions, the GlueX experiment will attempt to experimentally establish the spectrum of hybrid mesons. In this article, the present theoretical and experimental landscape is reviewed, the design of the GlueX detector presented, and the GlueX startup plans are briefly discussed.

  19. Finite Element Analysis of Magnetic Microparticle Induced Strain on a Fibrin Matrix due to the Influence of an Electromagnetic Field

    CERN Document Server

    Averett, Rodney D; Scogin, Tyler; Walker, Mitchell L R

    2016-01-01

    Blood clots occur in the human body when they are required to prevent bleeding. In pathological states such as diabetes and sickle cell disease, blood clots can also form undesirably due to hypercoagulable plasma conditions. With the continued effort in developing fibrin therapies for potential life-saving solutions, more mechanical modeling is needed to understand the properties of fibrin structures with inclusions. In this study, a fibrin matrix embedded with magnetic micro particles was subjected to a magnetic field to determine the plastic deformation of the clot. Using finite element analysis, we estimate the magnetic force from an electromagnet at a sample space located approximately 3 cm away from the coil center. This electromagnetic force along with gravity is applied on a fibrin sub model to calculate the stresses and displacements. Initial analyses show the forces are not sufficient to create fibrinolysis and hence we extended the study using parametric sweep analysis and redesign the coil paramete...

  20. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features.

    Science.gov (United States)

    Dohan, David M; Choukroun, Joseph; Diss, Antoine; Dohan, Steve L; Dohan, Anthony J J; Mouhyi, Jaafar; Gogly, Bruno

    2006-03-01

    Platelet-rich fibrin (PRF) belongs to a new generation of platelet concentrates, with simplified processing and without biochemical blood handling. In this second article, we investigate the platelet-associated features of this biomaterial. During PRF processing by centrifugation, platelets are activated and their massive degranulation implies a very significant cytokine release. Concentrated platelet-rich plasma platelet cytokines have already been quantified in many technologic configurations. To carry out a comparative study, we therefore undertook to quantify PDGF-BB, TGFbeta-1, and IGF-I within PPP (platelet-poor plasma) supernatant and PRF clot exudate serum. These initial analyses revealed that slow fibrin polymerization during PRF processing leads to the intrinsic incorporation of platelet cytokines and glycanic chains in the fibrin meshes. This result would imply that PRF, unlike the other platelet concentrates, would be able to progressively release cytokines during fibrin matrix remodeling; such a mechanism might explain the clinically observed healing properties of PRF.