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Sample records for autologe chondrozytentransplantation zur

  1. Indirect MR-arthography in the fellow up of autologous osteochondral transplantation; Indirekte MR-Arthrographie zur Verlaufskontrolle nach autologer osteochondraler Transplantation

    Energy Technology Data Exchange (ETDEWEB)

    Herber, S.; Pitton, M.B.; Kalden, P.; Thelen, M.; Kreitner, K.F. [Mainz Univ. (Germany). Klinik und Poliklinik fuer Radiologie; Runkel, M. [Mainz Univ. (Germany). Klinik und Poliklinik fuer Unfallchirurgie

    2003-02-01

    Purpose: To evaluate the spectrum of findings in indirect MR-arthrography following autologous osteochondral transplantation. Patients and Methods: 10 patients with autogenous osteochondral homografts underwent indirect MR-arthrography at three, 6 and 12 months postoperatively. The MR protocol at 1.5T comprised unenhanced imagings with PD- and T{sub 2}-weighted TSE-sequences with and without fat-suppression as well as T{sub 1}-weighted fat-suppressed SE-sequences before and after iv. contrast administration and after active joint exercise. Image analysis was done by two radiologists in conference and comprised the evaluation of signal intensity (SI) and integrity of the osseous plug and the cartilage surface, as well as the presence of joint effusion or bone marrow edema. Results: At three months, all cases demonstrated a significant bone marrow edema at the recipient and donor site that corresponded to a significant enhancement after iv. contrast administration. The interface between the transplant and the normal bone showed an increased SI at three and 6 months in T{sub 2}-weighted images as well as in indirect MR-arthrography. The marrow signal normalized in most cases after 6 to 12 months, indicating vitality and healing of the transplanted osteochondral graft. The SI of the interface decreased in the same period, demonstrating the stability of the homograft at the recipient site. The osteochondral plugs were well-seated in 9/10 cases. Indirect MR-arthrography was superior to unenhanced imaging in the assessment of the cartilage surface. Cartilage coverage was complete in every case. The transplanted hyaline cartilage as well as the original cartilage showed a significant increase of the SI in indirect MR-arthrography, that did not change in follow up studies. There were no pathological alterations of signal and thickness alterations of the transplanted cartilage in follow up investigations. Conclusion: Indirect MR-arthrography is a useful diagnostic tool

  2. Book Review: Internationale bibliographie zur germanistischen ...

    African Journals Online (AJOL)

    Book Review: Internationale bibliographie zur germanistischen lexikographie und wörterbuchforschung. mit berücksichtigung anglistischer, nordistischer, romanistischer, slavistischer und weiterer metalexikographischer forschungen.

  3. Neuer Beitrag zur Kenntnis aussereuropaeischer Trichopteren

    NARCIS (Netherlands)

    Ulmer, Georg

    1906-01-01

    In den folgenden Mitteilungen findet man alles veröffentlicht, was mir zur Zeit an neuem und wenig bekannten Materiale (mit Ausnahme amerikanischer Arten, für die mir keine Typen vorliegen) zur Verfügung steht. Herr Dr. H. W. van der Weele in Leiden war so freundlich, mir die aussereuropäischen

  4. Von der lernenden Region zur "Smart Region“

    OpenAIRE

    Poschwatta, Wolfgang

    2004-01-01

    Von der lernenden Region zur "Smart Region“ / M. Hilpert, W. Poschwatta. - In: Nabizadeh-Araghi, Nima : Auf dem Weg zur "Smart Region" : regionale Entwicklung am Beispiel der Pistazienproduktion im Iran. - Augsburg : Müllerdruck, 2004. - S. 5-9. - (Terra facta ; 2)

  5. Characterization of Zur-dependent genes and direct Zur targets in Yersinia pestis

    Directory of Open Access Journals (Sweden)

    Wang Xiaoyi

    2009-06-01

    Full Text Available Abstract Background The zinc uptake regulator Zur is a Zn2+-sensing metalloregulatory protein involved in the maintenance of bacterial zinc homeostasis. Up to now, regulation of zinc homeostasis by Zur is poorly understood in Y. pestis. Results We constructed a zur null mutant of Y. pestis biovar microtus strain 201. Microarray expression analysis disclosed a set of 154 Zur-dependent genes of Y. pestis upon exposure to zinc rich condition. Real-time reverse transcription (RT-PCR was subsequently used to validate the microarray data. Based on the 154 Zur-dependent genes, predicted regulatory Zur motifs were used to screen for potential direct Zur targets including three putative operons znuA, znuCB and ykgM-RpmJ2. The LacZ reporter fusion analysis verified that Zur greatly repressed the promoter activity of the above three operons. The subsequent electrophoretic mobility shift assay (EMSA demonstrated that a purified Zur protein was able to bind to the promoter regions of the above three operons. The DNase I footprinting was used to identify the Zur binding sites for the above three operons, verifying the Zur box sequence as predicted previously in γ-Proteobacteria. The primer extension assay was further used to determine the transcription start sites for the above three operons and to localize the -10 and -35 elements. Zur binding sites overlapped the -10 sequence of its target promoters, which was consistent with the previous observation that Zur binding would block the entry of the RNA polymerase to repress the transcription of its target genes. Conclusion Zur as a repressor directly controls the transcription of znuA, znuCB and ykgM-RpmJ2 in Y. pestis by employing a conserved mechanism of Zur-promoter DNA association as observed in γ-Proteobacteria. Zur contributes to zinc homeostasis in Y. pestis likely through transcriptional repression of the high-affinity zinc uptake system ZnuACB and two alternative ribosomal proteins YkgM and RpmJ2.

  6. Rebooting autoimmunity with autologous HSCT.

    Science.gov (United States)

    Snowden, John A

    2016-01-07

    Autologous hematopoietic stem cell transplantation (HSCT) is increasingly used for severe autoimmune and inflammatory diseases, but the mechanisms involved have yet to be elucidated. In this issue of Blood, Delemarre et al report their findings in both animal and human models which provide insights into restoration of functionality and diversity within the regulatory T-cell (Treg) compartment following HSCT.

  7. Der Weg von der Computerkompetenz zur Medienkompetenz

    Directory of Open Access Journals (Sweden)

    Barbara Zuliani

    2011-09-01

    Full Text Available Weiterführende Gedanken zur „Schlüsselkompetenz Nr.4“ des Europäischen Rates im Bereich des lebenslangen Lernens: Computerkompetenz bzw. Medienkompetenz, sowie Anwendungsbeispiele aus dem Bereich der Volksschule: Klassenblog und verschiedener Apps via iPad - 2 Praxisberichte

  8. Autologous Fat Injection for Augmented Mammoplasty

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Eul Sik; Seo, Bo Kyoung; Yi, Ann; Cho, Kyu Ran [Korea University Ansan Hospital, Ansan (Korea, Republic of)

    2008-12-15

    Autologous fat injection is one of the methods utilized for augmented mammoplasty methods. In this surgical procedure, the fat for transfer is obtained from the donor site of the patient's own body by liposuction and the fat is then injected into the breast. We report here cases of three patients who underwent autologous fat injection. Two of the patients had palpable masses that were present after surgery. The serial imaging findings and surgical method of autologous fat transfer are demonstrated

  9. Zur tariflichen Umsetzung des steuerlichen Existenzminimums

    OpenAIRE

    Baumann, Franz; Genser, Bernd

    1993-01-01

    Das Bundesverfassungsgericht erklärte in zwei Entscheidungen, 1990 den Kinderfreibetrag und 1992 den Grundfreibetrag des deutschen Einkommensteuergesetzes für zu niedrig und damit im Widerspruch zum Grundgesetz. In der vorliegenden Arbeit werden die international üblichen tarifpolitischen Instrumente zur Steuerfreistellung eines haushaltsbezogenen Existenzminimums hinsichtlich ihrer Verfassungskonformität sowie ihrer distributiven und fiskalischen Konsequenzen untersucht. Neben der reinen Tar...

  10. Zur odonatenfauna des Fintlandsmoores (Landkreis ammerland)

    OpenAIRE

    Gueffroy, Daniel; Liekweg, Tammo

    2000-01-01

    Zur Untersuchung der Libellenfauna des Fintlandsmoores im Landkreis Ammerland wurden 1999 zehn Exkursionen durchgeführt. Der Untersuchungsschwerpunkt lag im bereich der dystrophen Torfstiche im süden sowie des unabgetorften Hochmoorrestes im Zentrum des Naturschutzgebietes. Insgesamt wurden 15 Libellenarten als bodenständig nachgewiesen. Aufgrund früherer Untersuchungen aus den Jahren 1973-78 und 1986 läßt sich die zunehmende Eutrophierung anhand der Veränderung des Artenspektr...

  11. Autopoiesis: Autology, Autotranscendence and Autonomy

    DEFF Research Database (Denmark)

    , to the prospects of imagination, not least because imagination is driven by such paradoxes. The depth of the imagination is all surface, and all of its surfaces are deep structures. Schiltz’s exploration of autology grows out of one of a number (a growing number) of programmes that deal seriously with the idea......¿ning) problem of modernity. Castoriadis suggests a mutual and complementary relation between subjective and collective autonomy. Bouchet’s interpretation of this is very  radical and in certain respects quite startling. He considers how in modernity emerge spontaneous social orders (like markets or publics...

  12. Screening for autologous blood transfusions

    DEFF Research Database (Denmark)

    Mørkeberg, J; Belhage, B; Ashenden, M

    2009-01-01

    The ratio between the amount of hemoglobin in the mature erythrocyte population and the reticulocytes (RBCHb:RetHb ratio) has previously been suggested as a marker to screen for EPO-abuse. We speculated that the reinfusion of blood would lead to a marked increase in this ratio, making it a valuable...... parameter in the screening for autologous blood doping. Three bags of blood (approximately 201+/-11 g of Hb) were withdrawn from 16 males and stored at either -80 degrees C (-80 T, n=8) or +4 degrees C (+4 T, n=8) and reinfused 10 weeks or 4 weeks later, respectively. Seven subjects served as controls...... week wash-out period were identified as 'suspicious', and 18.8% (-80 T) and 4.3% (+4 T) as 'positive'. In total, 7 out of 16 (43.8%) subjects had at least one sample exceeding 182.9. Compared to the currently used indirect parameters, the RBCHb:RetHb ratio is the best indicator of autologous blood...

  13. Intraurethral Injection of Autologous Minced Skeletal Muscle

    DEFF Research Database (Denmark)

    Gräs, Søren; Klarskov, Niels; Lose, Gunnar

    2014-01-01

    PURPOSE: Intraurethral injection of in vitro expanded autologous skeletal muscle derived cells is a new regenerative therapy for stress urinary incontinence. We examined the efficacy and safety of a simpler alternative strategy using freshly harvested, minced autologous skeletal muscle tissue...... noted. CONCLUSIONS: Intraurethral injection of minced autologous muscle tissue is a simple surgical procedure that appears safe and moderately effective in women with uncomplicated stress urinary incontinence. It compares well to a more complicated regenerative strategy using in vitro expanded muscle...... with its inherent content of regenerative cells. MATERIALS AND METHODS: A total of 20 and 15 women with uncomplicated and complicated stress urinary incontinence, respectively, received intraurethral injections of minced autologous skeletal muscle tissue and were followed for 1 year. Efficacy was assessed...

  14. Hemifacial atrophy treated with autologous fat transplantation

    Directory of Open Access Journals (Sweden)

    Gandhi Vijay

    2005-01-01

    Full Text Available A 23-year-old male developed right hemifacial atrophy following marphea profunda. Facial asymmetry due to residual atrophy was treated with autologous fat harvested from buttocks with marked cosmetic improvement.

  15. Osteochondritis dissecans of the knee joint; Osteochondritis dissecans des Kniegelenks

    Energy Technology Data Exchange (ETDEWEB)

    Kutscha-Lissberg, F.; Singer, P. [Universitaetsklinik fuer Unfallchirurgie, Medizinische Universitaet Wien (Austria); Vecsei, V.; Marlovits, S. [Universitaetsklinik fuer Unfallchirurgie, Medizinische Universitaet Wien (Austria); Ludwig-Boltzmann-Institut fuer Biomechanik und Zellbiologie, Wien (Austria)

    2004-08-01

    Osteochondritis dissecans (OD) is an affection of the subchondral bone involving progressive detachment of an osteochondral fragment. This article describes the epidemiology, etiology, clinical appearance, possibilities for radiological diagnostics, and classification of OD and presents conservative and surgical therapeutic techniques. Treatment of OD depends on the size, location, stability of the fragment, and skeletal maturity. The majority of young patients can be treated conservatively. Surgical interventions include antegrade or retrograde drilling with optional refixation and osteochondral transplantation as well as autologous chondrocyte transplantation. The results of surgical intervention are quite promising; nevertheless, further prospective comparative studies are necessary to evaluate effectivity. (orig.) [German] Die Osteochondritis dissecans (OD) ist eine Erkrankung des subchondralen Knochens, die mit einer fortschreitenden Abloesung eines osteochondralen Fragments einhergeht. Dieser Beitrag beschreibt die Epidemiologie, Aetiologie, Klinik und Moeglichkeiten der radiologischen Diagnostik sowie die Klassifizierung der OD und stellt die konservativen und operativen Therapietechniken dar. Die Behandlung der OD ist von der Groesse, Lokalisation, Stabilitaet des Herdes und Skelettreife abhaengig. Die meisten juengeren Patienten koennen konservativ behandelt werden. Die chirurgischen Interventionen beinhalten die ante- oder retrograde Anbohrung mit optionaler Refixation und die osteochondrale Transplantation sowie autologe Chondrozytentransplantation. Die Ergebnisse der chirurgischen Intervention sind viel versprechend, trotzdem sind weiterfuehrende prospektiv vergleichende Studien zur Beurteilung der Effektivitaet notwendig. (orig.)

  16. Autologous fat transplantation for labia majora reconstruction.

    Science.gov (United States)

    Vogt, P M; Herold, C; Rennekampff, H O

    2011-10-01

    A case of autologous fat transplantation for labia majora augmentation after ablative surgery is presented. The patient reported pain and deformity of the left labium majus after resection for Bowen's disease. The symptoms had not been solved by classic plastic surgical reconstructions including a pudendal thigh fasciocutaneous flap. Use of autologous fat transplantation facilitated an improved aesthetic result while preserving residual sensation to the external genitalia and improving symptoms of mucosal exposure and dryness.

  17. [Autologous fat grafting and rhinoplasty].

    Science.gov (United States)

    Nguyen, P S; Baptista, C; Casanova, D; Bardot, J; Magalon, G

    2014-12-01

    Revision rhinoplasty can be very challenging especially in cases of thin skin. Autologous fat graft is utilized in numerous applications in plastic surgery; however, its use relative to the nasal region remains uncommon. Adipose tissue, by virtue of its volumetric qualities and its action on skin trophicity, can be considered to be a gold standard implant. From 2006 until 2012, we have treated patients by lipofilling in order to correct sequelae of rhinoplasty. The mean quantity of adipose tissue injected was 2.1cm(3) depending on the importance of the deformity and the area of injection: irregularity of the nasal dorsum, visible lateral osteotomies, saddle nose. Following the course of our practice, we conceived micro-cannulas that allow a much greater accuracy in the placement of the graft and enable to perform interventions under local anesthesia. These non-traumatic micro-cannulas do not cause post-operative ecchymosis and swelling which shorten the recovery time for the patient. On patients who have undergone multiple operations, lipofilling can be a simple and reliable alternative to correct imperfections that may take place after a rhinoplasty. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  18. Zur Relativität der Beschleunigung

    Science.gov (United States)

    Burghardt, R.

    Die Relativität der Beschleunigung wird im Lichte der Trederschen Tetradentheorie diskutiert. Die Erscheinungen des Newtonschen Eimerversuchs und Sagnac-Experiments werden feldtheoretisch untersucht und gezeigt, daß beide Experimente nicht im Widerspruch zur Relativität der Beschleunigung stehen. Das Prinzip der Konstanz der Lichtgeschwindigkeit behält auch im Rahmen der allgemeinen Relativitätstheorie seine Gültigkeit.Translated AbstractThe Relativity of AccelerationThe validity of the relativity of acceleration is investigated in view of Treder's theory of tetrads. Employing the methods of field theory, the effects of Newton's bucket and Sagnac's experiment are analyzed. The paper proves moreover that the results yielded by the experiments mentioned, do not contradict the basic ideas of the relativity of acceleration. The principle of constancy of light velocity is also valid in general relativity.

  19. Transabdominal sacrocolpopexy with autologous rectus fascia graft.

    Science.gov (United States)

    Abraham, Nitya; Quirouet, Adrienne; Goldman, Howard B

    2016-08-01

    Extrusion and infection are potential postoperative complications when using synthetic mesh for abdominal sacrocolpopexy. Long-term follow-up in the Colpopexy and Urinary Reduction Efforts (CARE) trial revealed an estimated 9.9 % risk of mesh extrusion. There are 26 reports of spondylodiscitis after sacrocolpopexy with synthetic mesh. These surgical risks may be decreased by using autologous fascia. To date, there have been no reports of extrusion or spondylodiscitis after using autologous fascia for sacrocolpopexy. This video demonstrates transabdominal sacrocolpopexy with an autologous rectus fascia graft. A 76-year-old woman with symptomatic stage 3 prolapse also had a history of diverticulitis and sigmoid abscess requiring sigmoid colectomy with end colostomy and incidental left ureteral transection with subsequent left nephrostomy tube placement. She presented for colostomy reversal, ureteral reimplantation, and prolapse repair. Given the need for concomitant colon and ureteral reconstruction, the risk of infection was potentially higher if synthetic mesh were used. The patient therefore underwent transabdominal sacrocolpopexy with autologous rectus fascia graft. At 4 months' follow-up the patient reported resolution of her symptoms and on examination she had no pelvic organ prolapse. Transabdominal sacrocolpopexy using autologous rectus fascia graft is a feasible option, especially in cases in which infection and synthetic mesh extrusion risks are potentially higher.

  20. Postoperative Autologous Reinfusion in Total Knee Replacement

    Directory of Open Access Journals (Sweden)

    A. Crescibene

    2015-01-01

    Full Text Available Surgeries for total knee replacement (TKR are increasing and in this context there is a need to develop new protocols for management and use of blood transfusion therapy. Autologous blood reduces the need for allogeneic blood transfusion and the aim of the present study was to verify the safety and the clinical efficacy. An observational retrospective study has been conducted on 124 patients, undergoing cemented total knee prosthesis replacement. Observed population was stratified into two groups: the first group received reinfusion of autologous blood collected in the postoperative surgery and the second group did not receive autologous blood reinfusion. Analysis of data shows that patients undergoing autologous blood reinfusion received less homologous blood bags (10.6% versus 30%; p=0.08 and reduced days of hospitalization (7.88 ± 0.7 days versus 8.96 ± 2.47 days for the control group; p=0.03. Microbiological tests were negative in all postoperatively salvaged and reinfused units. Our results emphasize the effectiveness of this procedure and have the characteristics of simplicity, low cost (€97.53 versus €103.79; p<0.01, and easy reproducibility. Use of autologous drainage system postoperatively is a procedure that allows reducing transfusion of homologous blood bags in patients undergoing TKR.

  1. Cloud Standby - Eine Methode zur Vorhaltung eines Notfallsystems in der Cloud

    OpenAIRE

    Lenk, Alexander

    2014-01-01

    Ziel dieser Arbeit ist es, eine neue Methode für die Vorhaltung eines Notfallsystems in der Cloud zu entwickeln, die aus einer Methode zur Notfallwiederherstellung und einer modellbasierten Deployment-Methode besteht. Die Methode zur Notfallwiederherstellung besteht aus einem Notfallwiederherstellungsprozess, eines Notfallwiederherstellungsprotokolls und einer Entscheidungsunterstützung zur Konfiguration des Prozesses besteht.

  2. Sites of Autologous Bone Grafts in Orthopaedic Traumatology ...

    African Journals Online (AJOL)

    Background: The use of autologous bone graft in orthopaedic traumatology is not uncommon. But little work, from West African subregion, has been devoted to sites used as sources of autologous bone grafts. Objective: The purpose of this study was to evaluate the evolution of these different sampling sites of autologous ...

  3. Autologous blood transfusion - a review | Charles | South African ...

    African Journals Online (AJOL)

    The discovery of HIV and other transfusion-transmissible infections has increased the demand for alternatives to allogeneic blood transfusion. One such alternative is autologous transfusion. This review presents an analysis of autologous transfusion. We conclude that autologous transfusion should form part of a strategy to ...

  4. Autologous fibrin adhesive in experimental tubal anastomosis.

    Science.gov (United States)

    Rajaram, S; Rusia, U; Agarwal, S; Agarwal, N

    1996-01-01

    To evaluate autologous fibrin in rabbit oviduct anastomosis versus 7-0 vikryl, a conventional suture material used in tubal anastomosis. Thrombin was added to the autologous fibrinogen at the site of anastomosis to obtain a tissue adhesive. The anastomotic time, pregnancy rate, and litter size were evaluated. Three months later, a relaparotomy was done to evaluate patency and degree of adhesions, and a tubal biopsy was taken from the site of anastomosis. Analysis of results showed a statistically significant (P < .001) shortened anastomotic time and superior histopathological union in the tissue adhesive group. Patency rate, pregnancy rate, and degree of adhesions were comparable in both groups.

  5. Cord Blood Banking Standards: Autologous Versus Altruistic

    Science.gov (United States)

    Armitage, Sue

    2016-01-01

    Cord blood (CB) is either donated to public CB banks for use by any patient worldwide for whom it is a match or stored in a private bank for potential autologous or family use. It is a unique cell product that has potential for treating life-threatening diseases. The majority of CB products used today are for hematopoietic stem cell transplantation and are accessed from public banks. CB is still evolving as a hematopoietic stem cell source, developing as a source for cellular immunotherapy products, such as natural killer, dendritic, and T-cells, and fast emerging as a non-hematopoietic stem cell source in the field of regenerative medicine. This review explores the regulations, standards, and accreditation schemes that are currently available nationally and internationally for public and private CB banking. Currently, most of private banking is under regulated as compared to public banking. Regulations and standards were initially developed to address the public arena. Early responses from the medical field regarding private CB banking was that at the present time, because of insufficient scientific data to support autologous banking and given the difficulty of making an accurate estimate of the need for autologous transplantation, private storage of CB as “biological insurance” should be discouraged (1, 2, 3). To ensure success and the true realization of the full potential of CB, whether for autologous or allogeneic use, it is essential that each and every product provided for current and future treatments meets high-quality, international standards. PMID:26779485

  6. Cord Blood Banking Standards: Autologous Versus Altruistic.

    Science.gov (United States)

    Armitage, Sue

    2015-01-01

    Cord blood (CB) is either donated to public CB banks for use by any patient worldwide for whom it is a match or stored in a private bank for potential autologous or family use. It is a unique cell product that has potential for treating life-threatening diseases. The majority of CB products used today are for hematopoietic stem cell transplantation and are accessed from public banks. CB is still evolving as a hematopoietic stem cell source, developing as a source for cellular immunotherapy products, such as natural killer, dendritic, and T-cells, and fast emerging as a non-hematopoietic stem cell source in the field of regenerative medicine. This review explores the regulations, standards, and accreditation schemes that are currently available nationally and internationally for public and private CB banking. Currently, most of private banking is under regulated as compared to public banking. Regulations and standards were initially developed to address the public arena. Early responses from the medical field regarding private CB banking was that at the present time, because of insufficient scientific data to support autologous banking and given the difficulty of making an accurate estimate of the need for autologous transplantation, private storage of CB as "biological insurance" should be discouraged (1, 2, 3). To ensure success and the true realization of the full potential of CB, whether for autologous or allogeneic use, it is essential that each and every product provided for current and future treatments meets high-quality, international standards.

  7. Predonated autologous blood transfusion in elective orthopaedic ...

    African Journals Online (AJOL)

    Background: The use of homologous blood carries significant risk of viral infections and immune-mediated reactions. Preoperative autologous blood donation is an attractive alternative to homologous transfusion and has become common in elective orthopaedic surgery. Objective: To present our experience with the use of ...

  8. Autologous Fat Grafting for Whole Breast Reconstruction

    Directory of Open Access Journals (Sweden)

    Benjamin H. L. Howes, MBBS

    2014-03-01

    Full Text Available Summary: This is the first reported case of a patient who had a single-stage large-volume breast reconstruction with autologous fat grafting, following rotation flap approach (RoFA mastectomy. The purpose of this case study was to evaluate the viability of reconstruction of the breast by autologous fat grafting alone, in the context of RoFA mastectomy. The hypothesis was that there would be minimal interval loss of autologous fat on the whole breast reconstruction side. Right RoFA mastectomy was used for resection of an invasive primary breast cancer and resulted in the right breast skin envelope. Eleven months later, the patient underwent grafting of 400 ml of autologous fat into the skin envelope and underlying pectoralis major muscle. Outcome was assessed by using a validated 3D laser scan technique for quantitative breast volume measurement. Other outcome measures included the BREAST-Q questionnaire and 2D clinical photography. At 12-month follow-up, the patient was observed to have maintenance of volume of the reconstructed breast. Her BREAST-Q scores were markedly improved compared with before fat grafting, and there was observable improvement in shape, contour, and symmetry on 2D clinical photography. The 2 new techniques, RoFA mastectomy and large-volume single-stage autologous fat grafting, were used in combination to achieve a satisfactory postmastectomy breast reconstruction. Novel tools for measurement of outcome were the 3D whole-body laser scanner and BREAST-Q questionnaire. This case demonstrates the potential for the use of fat grafting for reconstruction. Outcomes in a larger patient populations are needed to confirm these findings.

  9. Vergleich verschiedener Methoden zur Haftkraftmessung von Dentinadhäsiven

    OpenAIRE

    Schwertner, Katharina

    2010-01-01

    Zur Bestimmung der Haftkraft von Adhäsiven stehen verschiedene Methoden zur Verfügung. Ziel dieser Studie war es, vier gebräuchliche in vitro Haftkraftmessmethoden (Tensile Bond Strength Test, Shear Bond Strength Test, Microtensile Bond Strength Test, Push-out Bond Strength Test) an drei unterschiedlichen Adhäsivsystemen (Syntac, Clearfil SE Bond, Adper Scotchbond Multi Purpose) zu testen und die Ergebnisse zu vergleichen. Die Haftkräfte sind abhängig von der verwendeten Messmethode. Viele Pa...

  10. Von der Medienwirkungsbehauptung zur erziehungswissenschaftlichen Medienrezeptionsforschung. Vorschlag zur Analyse von Filmkommunikaten

    Directory of Open Access Journals (Sweden)

    Barbara Drinck

    2001-04-01

    Full Text Available Im Hinblick auf die Erforschung spezifischer Zusammenhänge von Medienkonsum und der Herausbildung von Einstellungen und Handlungen wird eine konstruktivistische Theorieorientierung plausibilisiert, indem zunächst auf den Stand der Medienwirkungsforschung eingegangen und vor dem Hintergrund einer begründeten Kritik des Wirkungsverständnisses auf die Notwendigkeit einer medienrezeptionswissenschaftlichen Forschungsalternative hingewiesen wird. Weiterhin wird der Stand der (erziehungswissenschaftlichen Medienrezeptionsforschung erörtert. Sodann werden Elemente einer konstruktivistischen Methodologie von Medienrezeptionsforschung beschrieben, die am Beispiel der Rezeption von Filmen konkretisiert werden. Dabei wird das Konzept des Kommunikates (S.J. Schmidt als Ausgangspunkt genommen, ein Vorschlag für eine Beschreibungssprache für (Film- Kommunikate entwickelt und eine Adaption des Ansatzes filmischer Narration zur Sprache gebracht.

  11. Glaube in der multireligiösen Gesellschaft: Zur Bildungskompetenz ...

    African Journals Online (AJOL)

    p1243322

    ),. Multikulturalität im vereinten Europa: Historische und juristische Aspekte, Göttingen 2003, S. 145-162, bes S 149f. A Grünschloß, Der eigene und der fremde Glaube. Studien zur interreligiösen Fremdwahrnehmung in Islam, Hinduismus, ...

  12. Autologous Fat Grafting Improves Facial Nerve Function

    Directory of Open Access Journals (Sweden)

    Marco Klinger

    2015-01-01

    Full Text Available We describe the case of a 45-year-old male patient who presented a retractile and painful scar in the nasolabial fold due to trauma which determined partial motor impairment of the mouth movements. We subsequently treated him with autologous fat grafting according to Coleman’s technique. Clinical assessments were performed at 5 and 14 days and 1, 3, and 6 months after surgical procedure and we observed a progressive release of scar retraction together with an important improvement of pain symptoms. A second procedure was performed 6 months after the previous one. We observed total restoration of mimic movements within one-year follow-up. The case described confirms autologous fat grafting regenerative effect on scar tissue enlightening a possible therapeutic effect on peripheral nerve activity, hypothesizing that its entrapment into scar tissue can determine a partial loss of function.

  13. Tumescent mastectomy technique in autologous breast reconstruction.

    Science.gov (United States)

    Vargas, Christina R; Koolen, Pieter G L; Ho, Olivia A; Ricci, Joseph A; Tobias, Adam M; Lin, Samuel J; Lee, Bernard T

    2015-10-01

    Use of the tumescent mastectomy technique has been reported to facilitate development of a hydrodissection plane, reduce blood loss, and provide adjunct analgesia. Previous studies suggest that tumescent dissection may contribute to adverse outcomes after immediate implant reconstruction; however, its effect on autologous microsurgical reconstruction has not been established. A retrospective review was conducted of all immediate microsurgical breast reconstruction procedures at a single academic center between January 2004 and December 2013. Records were queried for age, body mass index, mastectomy weight, diabetes, hypertension, smoking, preoperative radiation, reconstruction flap type, and autologous flap weight. Outcomes of interest were mastectomy skin necrosis, complete and partial flap loss, return to the operating room, breast hematoma, seroma, and infection. There were 730 immediate autologous breast reconstructions performed during the study period; 46% with the tumescent dissection technique. Groups were similar with respect to baseline patient and procedural characteristics. Univariate analysis revealed no significant difference in the incidence of mastectomy skin necrosis, complete or partial flap loss, return to the operating room, operative time, estimated blood loss, recurrence, breast hematoma, seroma, or infection in patients undergoing tumescent mastectomy. Multivariate analysis also demonstrated no significant association between the use of tumescent technique and postoperative breast mastectomy skin necrosis (P = 0.980), hematoma (P = 0.759), or seroma (P = 0.340). Use of the tumescent dissection technique during mastectomy is not significantly associated with adverse outcomes after microsurgical breast reconstruction. Despite concern for its impact on implant reconstruction, our findings suggest that this method can be used safely preceding autologous procedures. Copyright © 2015 Elsevier Inc. All rights reserved.

  14. Autologous Stem Cell Transplant for AL Amyloidosis

    OpenAIRE

    Roy, Vivek

    2012-01-01

    AL amyloidosis is caused by clonal plasma cells that produce immunoglobulin light chains which misfold and get deposited as amyloid fibrils. Therapy directed against the plasma cell clone leads to clinical benefit. Melphalan and corticosteroids have been the mainstay of treatment for a number of years and the recent availability of other effective agents (IMiDs and proteasome inhibitors) has increased treatment options. Autologous stem cell transplant (ASCT) has been used in the treatment of ...

  15. Siebenjährige Follow-up-Studie zur Anwendung des spannungsfreien Vaginalbandes (TVT zur Behandlung der Stressharninkontinenz

    Directory of Open Access Journals (Sweden)

    Nilsson CG

    2005-01-01

    Full Text Available Studienzweck: Evaluierung der langfristigen Heilungs- und Spätkomplikationsraten bei Stressharninkontinenz der Frau bei Behandlung mit dem spannungsfreien Vaginalband (TVT, das anhand einer minimal-invasiven Operation eingesetzt wird. Methodik: In drei Studienzentren durchgeführte prospektive Beobachtungs- und Kohortenstudie mit ursprünglich 90 Frauen, deren primäre Stressharninkontinenz einen operativen Eingriff erforderlich machte. Zu den Bewertungsvariablen gehörten ein Vorlagentest (Pad-Test zur Messung des Uringewichts je 24 Stunden, ein Stresstest, eine visuelle Analogskala (VAS zur Beurteilung des Beschwerdegrads sowie ein Fragebogen zur Beurteilung der subjektiven Wahrnehmung des Kontinenzstatus durch die Frauen. Ergebnisse: Als Follow-up-Zeitraum wurde ein durchschnittlicher Zeitraum von 91 Monaten (78–100 Monate festgelegt. Sowohl die objektive als auch die subjektive Heilungsrate lag bei den an der Follow-up-Studie teilnehmenden 80 Frauen bei 81,3 %. Bei 7,8 % der Frauen wurde ein asymptomatischer Prolaps der Organe des kleinen Beckens, bei 6,3 % De-novo-Drangsymptome und bei 7,5 % eine rezidivierende Harnwegsinfektion festgestellt. Weitere unerwünschte Nebenwirkungen des Verfahrens wurden nicht festgestellt. Schlussfolgerung: Die Wirkung der TVT-Operation zur Behandlung der Stressharninkontinenz der Frau bleibt über einen Zeitraum von 7 Jahren erhalten.

  16. Autologous growth factor injections in chronic tendinopathy.

    Science.gov (United States)

    Sandrey, Michelle A

    2014-01-01

    de Vos RJ, van Veldhoven PLJ, Moen MH, Weir A, Tol JL. Autologous growth factor injections in chronic tendinopathy: a systematic review. Br Med Bull. 2010;95:63-77. The authors of this systematic review evaluated the literature to critically consider the effects of growth factors delivered through autologous whole-blood and platelet-rich-plasma (PRP) injections in managing wrist-flexor and -extensor tendinopathies, plantar fasciopathy, and patellar tendinopathy. The primary question was, according to the published literature, is there sufficient evidence to support the use of growth factors delivered through autologous whole-blood and PRP injections for chronic tendinopathy? The authors performed a comprehensive, systematic literature search in October 2009 using PubMed, MEDLINE, EMBASE, CINAHL, and the Cochrane library without time limits. The following key words were used in different combinations: tendinopathy, tendinosis, tendinitis, tendons, tennis elbow, plantar fasciitis, platelet rich plasma, platelet transfusion, and autologous blood or injection. The search was limited to human studies in English. All bibliographies from the initial literature search were also viewed to identify additional relevant studies. Studies were eligible based on the following criteria: (1) Articles were suitable (inclusion criteria) if the participants had been clinically diagnosed as having chronic tendinopathy; (2) the design had to be a prospective clinical study, randomized controlled trial, nonrandomized clinical trial, or prospective case series; (3) a well-described intervention in the form of a growth factor injection with either PRP or autologous whole blood was used; and (4) the outcome was reported in terms of pain or function (or both). All titles and abstracts were assessed by 2 researchers, and all relevant articles were obtained. Two researchers independently read the full text of each article to determine if it met the inclusion criteria. If opinions differed on

  17. In vitro-Untersuchung zur Erreger-eliminierenden Wirkung von Wundspüllösungen

    OpenAIRE

    Nayel, Aria

    2010-01-01

    Die Durchführung einer gewissenhaften Lavage in Verbindung mit einem sorgfältig durchgeführten Débridement hat essenzielle Bedeutung im Wundmanagement der Akut-versorgung traumatologischer Patienten zur Verhinderung von Wundheilungsstörungen und etwaiger Infektionen. Für eine solche Lavage stehen diverse antiseptische Wundreinigungsmittel sowie sterile Spülmedien wie Kochsalz- oder Ringerlösung zur Verfügung. Zur Entschei...

  18. Repertory Grids als Methode zur Untersuchung von Schülervorstellungen im Bereich Computer und Internet

    OpenAIRE

    Pancratz, Nils

    2016-01-01

    In dieser Arbeit wird untersucht, inwieweit sich die Repertory Grid Methode zur Erhebung (bzw. zur Bestätigung bisheriger Ergebnisse von Untersuchungen) von Schülervorstellungen im Bereich Computer und Internet eignet. Dazu wird eine konkrete Repertory Grid Methode entwickelt und diese an einigen Schülerinnen und Schülern angewandt. Die Untersuchungen werden ausgewertet und mit bisherigen Ergebnissen verglichen. Abschließend wird die Eignung der Methode zur Untersuchung von Schülervorstellung...

  19. Use of autologous blood in general surgery.

    Science.gov (United States)

    D'Amato, A; Ferrazza, G; Solinas, S; Pronio, A M; Montesani, C; Ribotta, G

    2000-01-01

    Autologous blood predonation is still not as widespread as it should be in general surgery practice, even if the method is well-known and has benefits established in international literature. Authors describe the impact of an autotransfusion program, in a general surgery university department, focusing on management and cost problems. A description of the efficacy of the program during a yearlong activity period is presented. An analysis has been made about the quantity of predonated blood/plasma units, the quantity actually transfused and use of homologous blood. The problems which occurred and the cost are discussed. The most used autotransfusion method was preoperative predeposit of autologous blood. The analysis of results focused on some organizational problems that need to be avoided in order to show the methods maximum benefits. In a large number of cases (some 50%) predeposit was not made because of several managing/technical problems. In another large number of cases (38%) the quantity of units predonated did not fully supply the needs and several patients received homologous products. In another number of cases predonated blood units were not used at all (61/34%). Predeposit, preoperative hemodilution and intraoperative recovery, are methods that should all be available in a general surgery department to manage in the best way the single patients blood/plasma needs, reducing post-transfusion complication. To optimize the program and minimize waste some guidelines must be established, with the aim of a rational and correct use of the procedure. Despite the value of the method, and the favor encountered by the patients, we must not forget that the use of autologous blood is not costless.

  20. Cost effectiveness of autologous blood transfusion – A developing ...

    African Journals Online (AJOL)

    An autologous blood donation program was set up at National Orthopaedic Hospital, Igbobi Lagos in 1992 in response to the rising sero prevalence of HIV observed in our “relative replacement” donors. A retrospective batch analysis of patients who received autologous transfusion and those who received homologous ...

  1. Review of autologous blood transfusion at the Kenyatta National ...

    African Journals Online (AJOL)

    Autologous blood transfusion refers to transfusion of blood and/or blood components that are donated by the intended recipient (1). It is considered as one of the safest methods of blood transfusion (1,2). Different types of autologous blood include: preoperative blood deposit, preoperative haemodilution,intraope.

  2. Detection of accessory spleens with indium 111-labeled autologous platelets

    International Nuclear Information System (INIS)

    Davis, H.H. II; Varki, A.; Heaton, W.A.; Siegel, B.A.

    1980-01-01

    In two patients with recurrent immune thrombocytopenia, accessory splenic tissue was demonstrated by radionuclide imaging following administration of indium 111-labeled autologous platelets. In one of these patients, no accessory splenic tissue was seen on images obtained with technetium 99m sulfur colloid. This new technique provides a simple means for demonstrating accessory spleens and simultaneously evaluating the life-span of autologous platelets

  3. Disseminated Fusarium infection in autologous stem cell transplant recipient

    OpenAIRE

    Avelino-Silva, Vivian Iida; Ramos, Jessica Fernandes; Leal, Fabio Eudes; Testagrossa, Leonardo; Novis, Yana Sarkis

    2015-01-01

    Disseminated infection by Fusarium is a rare, frequently lethal condition in severely immunocompromised patients, including bone marrow transplant recipients. However, autologous bone marrow transplant recipients are not expected to be at high risk to develop fusariosis. We report a rare case of lethal disseminated Fusarium infection in an autologous bone marrow transplant recipient during pre-engraftment phase.

  4. Disseminated Fusarium infection in autologous stem cell transplant recipient

    Directory of Open Access Journals (Sweden)

    Vivian Iida Avelino-Silva

    2015-01-01

    Full Text Available Disseminated infection by Fusarium is a rare, frequently lethal condition in severely immunocompromised patients, including bone marrow transplant recipients. However, autologous bone marrow transplant recipients are not expected to be at high risk to develop fusariosis. We report a rare case of lethal disseminated Fusarium infection in an autologous bone marrow transplant recipient during pre-engraftment phase.

  5. Autologous Bone Grafts Use in Orthopaedic Practice in Abuja ...

    African Journals Online (AJOL)

    Background: There is widespread use of autologous bone grafts in orthopaedic practice in Nigeria but detailed indications, donor sites and complications following use have not been reported in different regions. Objective: This is to highlight the indications, sources and complications of autologous bone grafts use in Abuja, ...

  6. Modulares Leistungsberechnungsverfahren zur automatisierten modellbasierten Leistungsanalyse von Gasturbinen

    OpenAIRE

    Bauer, Michael

    2005-01-01

    Die Entwicklung moderner Gasturbinen wird durch ein umfangreiches Versuchsprogramm mit Prototypen begleitet. Zur Bewertung des Leistungs- und Betriebsverhaltens werden diese mit einer Vielzahl von Messwertaufnehmern bestückt, mit denen Drücke, Temperaturen, Drehzahlen usw. gemessen werden. Die Auswertung der Meßdaten erfolgt mit Hilfe von Analyseverfahren, die nicht gemessene Größen, wie z. B. Wirkungsgrade der Gasturbinenkomponenten und Turbinentemperaturen, unter Verwendung bekannter Zusamm...

  7. Oberflächenmodifizierung von Wirkstoffen zur Inhalation

    OpenAIRE

    Stank, Katharina

    2014-01-01

    In der vorliegenden Arbeit wird die Anwendung des „dry particle coating“ für die Oberflächenmodifizierung von mikronisiertem Salbutamolsulfat zur Inhalation beschrieben. Bei diesem Verfahren wird der Wirkstoff durch einen mechanischen Energieeintrag mit einem Hilfsstoff verbunden, um dadurch Veränderungen der Oberflächeneigenschaften zu erzielen. Der Einfluss der Oberflächenmodifizierung wurde sowohl für trägerfreie Pulverformulierungen als auch für suspensionsbasierte Dosieraerosole untersuc...

  8. Untersuchung zur Fixierung von Knorpelgewebe mittels laserinduzierter Koagulation

    OpenAIRE

    Hoffmann, Philipp

    2012-01-01

    Philipp Hoffmann Untersuchung zur Fixierung von Knorpelgewebe mittels laserinduzierter Koagulation Aus der Chirurgischen Tierklinik der Veterinärmedizinische Fakultät der Universität Leipzig, angefertigt im Forschungszentrum für Medizintechnik und Biotechnologie GmbH, Bad Langensalza Eingereicht im Januar 2012 97 Seiten, 59 Abbildungen, 9 Tabellen, 318 Literaturangaben 10 Seiten Anhang Schlüsselwörter: Laser, Löten, Knorpelgewebe, Zugfestigkeit, thermische Schäden Gelenkerk...

  9. Rechtliches Verfahrensmanagement in komplexen Verfahren zur Schaffung von Baurecht

    OpenAIRE

    Miller, Karin

    2017-01-01

    Kurzfassung: Die Arbeit befasst sich mit einem Thema, das an der Schnittstelle zwischen klassischer Rechtsberatung in vornehmlich projektorientierten Bebauungsplanverfahren, Bauleitplanung und Projektsteuerung angesiedelt ist und behandelt den Aspekt, dass sich der Ablauf derartiger Verfahren hin zu ständig steigender Komplexität entwickelt. Eine in das Thema einführende Analyse stellt dar, dass diese Komplexität nicht nur auf das eigentliche Planaufstellungsverfahren zurück zu führe...

  10. Complications Following Autologous Latissimus Flap Breast Reconstruction

    Directory of Open Access Journals (Sweden)

    Mufid Burgić

    2010-02-01

    Full Text Available Use of an autologous latissimus flap in breast reconstruction accounts for a supple and natural look of reconstructed breast. Most common postoperative complication, seroma, became more of a rule then an exception when it comes to postoperative evaluation of the patients who underwent this reconstructive procedure. A retrospective study analysing and evaluating different complication rates in 20 patients who underwent breast reconstruction by autologous latissimus flap, was conducted. All patients included in the study were operated at the Department of plastic surgery of Hôpital Civil in Strasbourg, France, between 1996 and 2008. The complication rates were noted as follows: seroma in 19 of our 20 patients (95%, late hypertrophic scarring in 3 patients (15%, postoperative surgical site hematoma in 3 patients (15%, and 2 patients (10% presented postoperative chronic back pain. Different options used in seroma treatment and prevention (subcutaneous-fascia anchor sutures of donor site, application of corticosteroids by injection into donor site postoperatively, passive drainage can reduce seroma formation and thus overall complication rates, leading to much faster patient’s recovery time and return to normal daily activities.

  11. Cartilage repair: Generations of autologous chondrocyte transplantation

    Energy Technology Data Exchange (ETDEWEB)

    Marlovits, Stefan [Department of Traumatology, Center for Joint and Cartilage, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna (Austria)]. E-mail: stefan.marlovits@meduniwien.ac.at; Zeller, Philip [Department of Traumatology, Center for Joint and Cartilage, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna (Austria); Singer, Philipp [Department of Traumatology, Center for Joint and Cartilage, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna (Austria); Resinger, Christoph [Department of Traumatology, Center for Joint and Cartilage, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna (Austria); Vecsei, Vilmos [Department of Traumatology, Center for Joint and Cartilage, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna (Austria)

    2006-01-15

    Articular cartilage in adults has a limited capacity for self-repair after a substantial injury. Surgical therapeutic efforts to treat cartilage defects have focused on delivering new cells capable of chondrogenesis into the lesions. Autologous chondrocyte transplantation (ACT) is an advanced cell-based orthobiologic technology used for the treatment of chondral defects of the knee that has been in clinical use since 1987 and has been performed on 12,000 patients internationally. With ACT, good to excellent clinical results are seen in isolated post-traumatic lesions of the knee joint in the younger patient, with the formation of hyaline or hyaline-like repair tissue. In the classic ACT technique, chondrocytes are isolated from small slices of cartilage harvested arthroscopically from a minor weight-bearing area of the injured knee. The extracellular matrix is removed by enzymatic digestion, and the cells are then expanded in monolayer culture. Once a sufficient number of cells has been obtained, the chondrocytes are implanted into the cartilage defect, using a periosteal patch over the defect as a method of cell containment. The major complications are periosteal hypertrophy, delamination of the transplant, arthrofibrosis and transplant failure. Further improvements in tissue engineering have contributed to the next generation of ACT techniques, where cells are combined with resorbable biomaterials, as in matrix-associated autologous chondrocyte transplantation (MACT). These biomaterials secure the cells in the defect area and enhance their proliferation and differentiation.

  12. Alexandru Popa, Untersuchungen zu den römisch-barbarischen Kontakten Östlich der römischen Provinz Dacia Antiquitatis reihe 3 Abhnadlungen zur vor-und frühgeschichte, zur klassichen und provinzial-römischen archäologie und zur geschichte des altertum

    Directory of Open Access Journals (Sweden)

    Vasile Iarmulschi

    2016-06-01

    Full Text Available Review on book - Alexandru Popa, Untersuchungen zu den römisch-barbarischen Kontakten Östlich der römischen Provinz Dacia Antiquitatis reihe 3 Abhnadlungen zur vor-und frühgeschichte, zur klassichen und provinzial-römischen archäologie und zur geschichte des altertum

  13. Strain Rate Imaging- eine neue Methode zur Quantifizierung der regionalen Myokardfunktion Prospektive, longitudinale Studie zur Erstellung von Referenzwerten im Neugeborenenalter

    OpenAIRE

    Hornung, Andreas

    2008-01-01

    Einleitung: Strain Rate Imaging (SRI) ist eine neue Methode zur Quantifizierung der regionalen myokardialen Funktion. Basierend auf der myokardialen Dopplersonographie wird die lokale Verkürzungsgeschwindigkeit berechnet. Normwerte existieren bislang nur für ältere Kinder und Erwachsene. Ziel war die Erstellung von Referenzwerten für das Neugeborenenalter und der Vergleich mit publizierten Normwerten anderer Altersgruppen. Methode: Im Rahmen einer prospektiven longitudinalen Studie mit 50...

  14. Predeposit autologous blood transfusion: Do we require to promote it?

    Directory of Open Access Journals (Sweden)

    Gurjit Singh

    2015-01-01

    Full Text Available Introduction: Safest blood a patient can receive is his own. Quest for safe blood transfusion has remained of prime concern. To meet this aspiration, various forms of autologous blood transfusions can be practiced. It is especially suitable for patients with rare blood groups and religious sects such as Jehovah′s witness autologous transfusion is extremely safe. Cross matching is not required; iso-immunization to a foreign body is excluded. Fear of transfusion transmissible disease can be ignored. Therefore, autologous blood transfusion is required to be revisited. Materials and Methods: This is a prospective study carried out at Padmashree Dr. D. Y. Patil Medical College, Hospital and Research Centre, Pune between July 2010 and May 2012. Study comprised of 100 patients divided into two groups, autologous and homologous. Benefits of autologous transfusion were studied. Results: There was no significant change in hematocrit and blood parameters after blood donation. That is mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration (P < 0.001 after blood donation. Only one complication of vasovagal syncope was observed at the time of blood donation. Conclusion: Autologous blood transfusion is safe. Easy alternative to be practiced in elective surgeries, especially in patients with rare blood group or believers of Jehovah′s witness faith. It helps to reduce the shortfall in national blood inventory. Autologous blood donation should be practiced whenever possible.

  15. Autologous Stem Cell Transplant for AL Amyloidosis

    Directory of Open Access Journals (Sweden)

    Vivek Roy

    2012-01-01

    Full Text Available AL amyloidosis is caused by clonal plasma cells that produce immunoglobulin light chains which misfold and get deposited as amyloid fibrils. Therapy directed against the plasma cell clone leads to clinical benefit. Melphalan and corticosteroids have been the mainstay of treatment for a number of years and the recent availability of other effective agents (IMiDs and proteasome inhibitors has increased treatment options. Autologous stem cell transplant (ASCT has been used in the treatment of AL amyloidosis for many years. It is associated with high rates of hematologic response and improvement in organ function. However, transplant carries considerable risks. Careful patient selection is important to minimize transplant related morbidity and mortality and ensure optimal patient outcomes. As newer more affective therapies become available the role and timing of ASCT in the overall treatment strategy of AL amyloidosis will need to be continually reassessed.

  16. Surgical management and autologous intestinal reconstruction in short bowel syndrome

    NARCIS (Netherlands)

    Hommel, Matthijs J.; van Baren, Robertine; Haveman, Jan Willem

    Short bowel syndrome (SBS) is a serious condition with considerable morbidity and mortality. When treatment with parenteral nutrition fails and life-threatening complications occur, autologous intestinal reconstruction (AIR) should be considered before intestinal transplantation (ITx). Single or

  17. Autologous bone marrow mononuclear cell delivery to dilated ...

    African Journals Online (AJOL)

    Autologous bone marrow mononuclear cell delivery to dilated cardiomyopathy patients: A clinical trial. PLN Kaparthi, G Namita, LK Chelluri, VSP Rao, PK Shah, A Vasantha, SK Ratnakar, K Ravindhranath ...

  18. Von der konventionellen laparoskopischen Prostatektomie zur DaVinci-Prostatektomie

    Directory of Open Access Journals (Sweden)

    Fehr JL

    2006-01-01

    Full Text Available Die Operationstechnik der radikalen Prostatektomie hat sich in den letzten 20 Jahren grundlegend verändert. Einerseits hat sich die Technik verfeinert und berücksichtigt die neuesten anatomischen Erkenntnisse der Prostata (Sphinkterapparat, Gefäßnervenbahnen, andererseits hat sich der minimalinvasive Zugang mittels Laparoskopie etabliert und findet nun mit der Roboterunterstützung (DaVinci-Technologie eine weitere Verbreitung. Die Autoren berichten über ihre Erfahrungen beim Wechsel von der konventionellen laparoskopischen Prostatektomie zur laparoskopischen DaVinci-Prostatektomie.

  19. Studien zur Synthese von steroidrezeptor-bindenden Phototherapeutika

    OpenAIRE

    Orth, Christian Michael

    2004-01-01

    In den letzten Jahren gewann die Photodynamische Therapie deutlich an Bedeutung bei der Behandlung von Neoplasien. Für die nächsten Jahren werden weitere Zulassungen für neue Indikationen erwartet. Diese Neuzulassungen werden einerseits durch neue Photosensibilisatoren und andererseits durch neue Ansätze beim Drug Targeting ermöglicht. Zur Zeit wird der Ansatz forciert, die Sensibilisatoren an einen tumorspezifischen Antikörper zu knüpfen. Eine weitere Möglichkeit für das Drug Targeting beste...

  20. Inflammatory effects of autologous, genetically modified autologous, allogeneic, and xenogeneic mesenchymal stem cells after intra-articular injection in horses.

    Science.gov (United States)

    Pigott, J H; Ishihara, A; Wellman, M L; Russell, D S; Bertone, A L

    2013-01-01

    To compare the clinical and inflammatory joint responses to intra-articular injection of bone marrow-derived mesenchymal stem cells (MSC) including autologous, genetically modified autologous, allogeneic, or xenogeneic cells in horses. Six five-year-old Thoroughbred mares had one fetlock joint injected with Gey's balanced salt solution as the vehicle control. Each fetlock joint of each horse was subsequently injected with 15 million MSC from the described MSC groups, and were assessed for 28 days for clinical and inflammatory parameters representing synovitis, joint swelling, and pain. There were not any significant differences between autologous and genetically modified autologous MSC for synovial fluid total nucleated cell count, total protein, interleukin (IL)-6, IL-10, fetlock circumference, oedema score, pain-free range-of-motion, and soluble gene products that were detected for at least two days. Allogeneic and xenogeneic MSC produced a greater increase in peak of inflammation at 24 hours than either autologous MSC group. Genetically engineered MSC can act as vehicles to deliver gene products to the joint; further investigation into the therapeutic potential of this cell therapy is warranted. Intra-articular MSC injection resulted in a moderate acute inflammatory joint response that was greater for allogeneic and xenogeneic MSC than autologous MSC. Clinical management of this response may minimize this effect.

  1. Spalthauttransplantation zur Defektdeckung am Ohr: eine statistische Analyse.

    Science.gov (United States)

    Thuile, Tobias; Larcher, Lorenz; Gatscher, Barbara; Schwaiger, Karl; Deluca, Jenny; Fallaha, Amgiad; Tappeiner, Lukas; Puviani, Mario; Eisendle, Klaus

    2018-02-01

    Die Rekonstruktion nach Entfernung ausgedehnter maligner Hauttumoren am Ohr ist eine Herausforderung. Verschiedene Methoden der Defektdeckung wurden beschrieben. Lappenplastiken, längere Anästhesiezeiten, geringe Compliance und Antikoagulation stellen bei den oft älteren Patienten zusätzliche Risiken dar. Eine rasch, effizient und unkompliziert durchführbare Methode der Defektdeckung ist daher wünschenswert. Ziel der Studie war die Beurteilung des ästhetischen Resultats, des Heilungsprozesses, der Komplikations- und Rezidivrate nach Transplantation ungenetzter Spalthaut (SPHT) zur Defektdeckung nach knorpelerhaltender Resektion maligner Tumoren am Ohr. 32 Patienten wurden nach Entfernung von Hauttumoren am Ohr einer Defektdeckung mittels SPHT unterzogen. Die durchschnittliche Defektgröße betrug 8,0 cm 2 . Eine vollständige Einheilung des Transplantates erfolgte in allen Fällen innerhalb von zwei Wochen. Wesentliche Komplikationen traten nicht auf. Das ästhetische Resultat wurde sowohl von den Patienten als auch von einem Dermatochirurgen und zwei plastischen Chirurgen als gut bewertet. Die SPHT am Ohr ist eine zuverlässige Methode zur Deckung ausgedehnter Hautdefekte. Selbst bei großen Tumoren des äußeren Ohres ist der Ohrknorpel nicht immer infiltriert. Eine knorpelschonende Tumorresektion mit anschließender Defektdeckung mittels SPHT ist eine ausgezeichnete und rasch durchführbare Technik mit hoher Patientenzufriedenheit. © 2018 Deutsche Dermatologische Gesellschaft (DDG). Published by John Wiley & Sons Ltd.

  2. Autologous fat grafting: use of closed syringe microcannula system for enhanced autologous structural grafting

    Directory of Open Access Journals (Sweden)

    Alexander RW

    2013-04-01

    Full Text Available Robert W Alexander,1 David Harrell2 1Department of Surgery, School of Medicine and Dentistry, University of Washington, Seattle, WA, USA; 2Harvest-Terumo Inc, Plymouth, MA, USA Objectives: Provide background for use of acquiring autologous adipose tissue as a tissue graft and source of adult progenitor cells for use in cosmetic plastic surgery. Discuss the background and mechanisms of action of closed syringe vacuum lipoaspiration, with emphasis on accessing adipose-derived mesenchymal/stromal cells and the stromal vascular fraction (SVF for use in aesthetic, structural reconstruction and regenerative applications. Explain a proven protocol for acquiring high-quality autologous fat grafts (AFG with use of disposable, microcannula systems. Design: Explain the components and advantage of use of the patented super luer-lock and microcannulas system for use with the closed-syringe system. A sequential explanation of equipment selection for minimally traumatic lipoaspiration in small volumes is presented, including use of blunt injection cannulas to reduce risk of embolism. Results: Thousands of AFG have proven safe and efficacious for lipoaspiration techniques for large and small structural fat grafting procedures. The importance and advantages of gentle harvesting of the adipose tissue complex has become very clear in the past 5 years. The closed-syringe system offers a minimally invasive, gentle system with which to mobilize subdermal fat tissues in a suspension form. Resulting total nuclear counting of undifferentiated cells of the adipose-derived -SVF suggests that the yield achieved is better than use of always-on, constant mechanical pump applied vacuum systems. Conclusion: Use of a closed-syringe lipoaspiration system featuring disposable microcannulas offers a safe and effective means of harvesting small volumes of nonmanipulated adipose tissues and its accompanying progenitor cells within the SVF. Closed syringes and microcannulas are

  3. Autologous Blood Transfusion for Postpartum Hemorrhage.

    Science.gov (United States)

    Greenawalt, Julia A; Zernell, Denise

    Postpartum hemorrhage (PPH) is a leading contributor to maternal morbidity and mortality in the United States and globally. Although the rate of PPH is generally decreasing nationally, severity of PPH appears to be increasing, potentially related to the various comorbidities associated with women of childbearing age. There is increasing evidence of risks associated with allogeneic blood transfusion, which has historically been the classic therapeutic approach for treatment to PPH. Pregnant women are particularly susceptible to the implications of sensitization to red cell antigens, a common sequela to allogenic blood transfusion. Autologous blood transfusion eliminates the potential of communicable disease transmission as well as the conceivable threat of a blood transfusion reaction. Recent technological advances allow cell salvage coupled with the use of a leukocyte filter to be used as an alternative approach for improving the outcome for women experiencing a PPH. Modest changes in standard operating procedure and continued training in use and application of cell salvaged blood may assist in minimizing negative outcomes from PPH. Salvaged blood has been demonstrated to be at least equal and often superior to banked blood. We discuss nursing implications for application of this technology for women with PPH. Continued research is warranted to evaluate the impact that application of cell salvage with filtration has on the patient experiencing a PPH.

  4. [Significance and technique of autologous chondrocyte transplantation].

    Science.gov (United States)

    Fritz, J; Gaissmaier, C; Schewe, B; Weise, K

    2005-08-01

    The bad risk for an early onset of osteoarthritis in the knee increases with the size of a cartilage defect. A collateral meniscus- or ligament-tear will enforce this hazard in addition. In order to avoid such a development, relevant full-thickness cartilage defects should be reconstructed biologically and attendant meniscus- or ligament-tears as well as varus- or valgus deformities should be treated. A number of studies, including some prospective-randomized trials, have shown that autologous chondrocyte transplantation (ACT) is the most reliable procedure for a surgical treatment of full-thickness cartilage defects larger than 4 cm (2) in adults. One disadvantage of ACT is the extensive approach to the joint and often a hypertrophy of the repair tissue. To solve these problems, some different biomaterials for a matrix-assisted ACT have been developed. The scaffold we use has a covering membrane upside and a collagen-sponge carrying the chondrocytes. By means of special surgical instruments a minimally invasive implantation is possible, reducing the side-effects of an extensive approach. Animal studies showed the regeneration of a hyaline cartilage using our described system. However, results of current clinical studies with the different scaffolds must be awaited before an universal application of matrix-assisted ACT can be recommended.

  5. Contributions to ecosystem research and environmental education III; Beitraege zur Oekosystemforschung und Umwelterziehung III

    Energy Technology Data Exchange (ETDEWEB)

    Erdmann, K.H. [ed.; Nauber, J. [ed.

    1996-09-01

    The publication comprises the following chapters: Contributions to the implementation of findings in policy and administration; Contributions to biosphere reservations; Contributions to ecosystems research; Contributions to environmental education. The first chapter discusses environmental protection from the political point of view, while the second chapter describes biosphere reservations world-wide as well as their protection, conservation and development. (SR) [Deutsch] Die vorliegende Veroeffentlichung teilt sich in folgende Kapitel auf: Beitraege zur Umsetzung von Erkenntnissen in Politik und Verwaltung, Beitraege zu Biosphaerenreservaten, Beitraege zur Oekosystemforschung sowie Beitraege zur Umwelterziehung. Im ersten Kapitel wird der Umweltschutz von der politischen Seite aus beleuchtet. Das zweite Kapitel beschreibt verschiedene Biosphaerenreservate in der ganzen Welt und deren Schutz, Pflege und Entwicklung. Das dritte Kapitel beinhaltet Beitraege zur oekologischen Forschung. Abschliessend werden im letzten Kapitel psychologische Aspekte der Umwelterziehung dargestellt. (SR)

  6. [Alfred Ritscher. Reval an der schwelle zur neuzeit. T. 2] / Paul Kaegbein

    Index Scriptorium Estoniae

    Kaegbein, Paul

    2006-01-01

    Arvustus: Ritscher, Alfred. Reval an der schwelle zur neuzeit. T. 2: Vom tode Wolters von Plettenberg bis zum Untergang des Deutschen Ordens in Livland (1535-1561). Bonn: Kulturstiftung der deutshen Vertriebenen 2001. (Historische Forschungen).

  7. Der gläserne Aschenbecher - oder - Zur Verallgemeinerung des Eulerschen Polyedersatzes

    OpenAIRE

    Gallin, P

    2009-01-01

    Eine Diskussion unter Mathematikern im Lehrerzimmer einer Kantonsschule führt zusammen mit Beiträgen der Schülerinnen und Schüler unversehens zu topologischen Einsichten, die normalerweise im Gymnasium nicht zur Sprache kommen.

  8. Quellen zur Geschichte der Volksbibliotheken in Württemberg und Hohenzollern 1806-1918

    OpenAIRE

    Hohoff, Ulrich

    1990-01-01

    Quellen zur Geschichte der Volksbibliotheken in Württemberg und Hohenzollern 1806-1918 : e. sachthemat. Inventar / bearb. von Ulrich Hohoff. - Stuttgart : Kohlhammer, 1990. - 357 S. - (Veröffentlichungen der Staatlichen Archivverwaltung Baden-Württemberg ; 40)

  9. Entwicklung einer Methodik zur Generierung einer Datenbasis für Optimierungssysteme in der maritimen Leercontainerlogistik

    OpenAIRE

    Vojdani, Nina; Rösner, René

    2013-01-01

    Dieser Beitrag stellt ein Vorgehen zur Entwicklung einer Methodik zur Generierung einer praxisnahen Datenbasis für numerische Untersuchungen im Rahmen der maritimen Leercontainerlogistik vor. Das Vorgehen wird an einem exemplarischen Anwendungsfall verdeutlicht. Die Resultate sollen Testläufe für Szenarien der Leercontainerlogistik unterstützen und somit eine Basis für die Entwicklung und Bewertung organisatorischer Verbesserungsansätze, mathematischer Optimierungsmodelle, entsprechender Lösu...

  10. Competitive Intelligence : Aufbau einer Informationsbasis und Entscheidungssystematik zur Definition strategischer Zielmärkte

    OpenAIRE

    Maffenbeier, Sina

    2010-01-01

    Im Rahmen der Globalisierung und des daraus resultierenden Wettbewerbs ist es für ein Unternehmen von zentraler Bedeutung, Wissen über die Wettbewerbssituation zu erhalten. Nicht nur zur Erschließung neuer Märkte, sondern auch zur Sicherung der Unternehmensexistenz ist eine Wettbewerbsanalyse unabdingbar. Konkurrenz- bzw. Wettbewerbsforschung wird überwiegend als „Competitive Intelligence“ bezeichnet. In diesem Sinne beschäftigt sich die vorliegende Bachelorarbeit mit einem Bereich von Compet...

  11. Repetitive transkranielle Magnetstimulation zur Tinnitusbehandlung: keine Verbesserung der Effekte durch dopaminerge Rezeptorstimulation

    OpenAIRE

    Kleinjung, T; Vielsmeier, V; Frank, E; Burger, JS; Landgrabe, M; Hajak, G; Strutz, J; Langguth, B

    2010-01-01

    Einleitung: Die repetitive transkranielle Magnetstimulation (rTMS) stellt ein Instrument zur fokalen Modulation neuronaler Aktivität dar. Niedrigfrequente rTMS über dem auditorischen Kortex wird erfolgreich zur Reduktion des Tinnitusschweregrades eingesetzt. Allerdings sind die positiven Effekte als moderat zu bezeichnen und weisen eine hohe interindividuelle Streuung auf. Untersuchungen an gesunden Probanden konnten zeigen, dass die Effekte von rTMS im Bereich des Motorcortex durch gleichze...

  12. Altautoverwertung zwischen Staat und Markt: Bedingungen und Potentiale zur Modernisierung von Lagerhaltung und Marketing gebrauchter Autoteile

    OpenAIRE

    Lucas, Rainer

    2000-01-01

    Das Arbeitspapier diskutiert vor dem Hintergrund veränderter gesetzlicher Rahmen-und Wettbewerbsbedingungen in der Autoverwertung Ziele und Maßnahmen zurStärkung kleiner und mittelständischer Verwertungsbetriebe. Der Bereich GebrauchteAutoteile wird im Rahmen eines regionalen Fallbeispiels einer tiefergehenden Analyseunterzogen. Hieraus werden Vorschläge zur regionalen Netzwerkbildung innerhalb derBranche und neue Dienstleistungsperspektiven abgeleitet. Ein wesentliches Koope-rationsfeld ist ...

  13. Cryopreservation of Autologous Blood (Red Blood Cells, Platelets and Plasma)

    Science.gov (United States)

    Ebine, Kunio

    Prevention of post-transfusion hepatitis is still a problem in cardiovascular surgery. We initiated the cryopreservation of autologous blood for the transfusion in elective cardiovascular surgery since 1981. This study includes 152 surgical cases in which autologous frozen, allogeneic frozen, and/or allogeneic non-frozen blood were used. In the 152 surgical cases, there were 69 cases in which autologous blood only (Group I) was used; 12 cases with autologous and allogeneic frozen blood (Group II); 46 cases with autologous and allgeneic frozen plus allogeneic non-frozen blood (Group III); and 25 cases with allogeneic frozen plus allogeneic non-frozen blood (Group IV). No hepatitis developed in Groups I (0%) and II (0%), but there was positive hepatitis in Groups III (4.3%) and IV (8.0%) . In 357 cases of those who underwent surgery with allogeneic non-frozen whole blood during the same period, the incidence rate of hepatitis was 13.7% (49/357). Patients awaiting elective surgery can store their own blood in the frozen state. Patients who undergo surgery with the cryoautotransfusion will not produce any infections or immunologic reactions as opposed to those who undergo surgery with the allogeneic non-frozen blood.

  14. Spanish Experience in Autologous Chondrocyte Implantation

    Science.gov (United States)

    Pérez-Cachafeiro, Santiago; Ruano-Raviña, Alberto; Couceiro-Follente, José; Benedí-Alcaine, Jose Antonio; Nebot-Sanchis, Ignacio; Casquete-Román, Ciriaco; Bello-Prats, Santiago; Couceiro-Sánchez, Gonzalo; Blanco, Francisco J.

    2010-01-01

    Introduction: The Spanish Ministry of Health commissioned the Galician Agency for Health Technology Assessment to monitor and follow-up Autologous Chondrocyte Implantation (ACI) used to treat chondral lesions of the knee in Spain. The objective of this monitoring was to assess efficacy and safety of the technique. Design: One-hundred and eleven consecutive patients with knee chondral lesions were included in a multi-center study between January 2001 and January 2005. ACI was used in these patients as a second-line treatment option (or a first-line treatment option if the cause was Osteocondritis dissecans). The Cincinnati score and the Short Form 36 (SF-36) questionnaire were used to assess the patients’ self-reported satisfaction with the outcomes of ACI. A descriptive analysis was performed and non-parametric tests were used to establish correlations and compare results among subgroups. A multivariate analysis was also performed to measure the effect of different variables on changes in the condition of the knee. Results: Eighty men (72%) and 31 women (21%) with an age range from 16 to 49 years, underwent ACI surgery. Among these subjects, the most common previous first-line treatment was debridement (64 individuals, 74.4%). The mean size of the lesion treated with ACI was 3.82 cm2, and the most frequent location of the lesion was the inner femoral condyle (53.6%). The patient satisfaction was high or very high in 36 subjects (66.7%). Overall knee joint assessment improved from 4.32 points to 6.78. All SF-36 questionnaire categories improved, notably those related to physical condition. Conclusions: The results of this study indicate that ACI is safe; however, further studies are mandated to assess the efficacy of ACI compared to alternative treatment options. PMID:20148094

  15. Effectiveness of autologous transfusion system in primary total hip and knee arthroplasty.

    LENUS (Irish Health Repository)

    Schneider, Marco M

    2014-01-01

    Autologous transfusion has become a cost-efficient and useful option in the treatment of patients with high blood loss following major orthopaedic surgery. However, the effectiveness of autologous transfusion in total joint replacement remains controversial.

  16. Recommended procedure for selection of detector systems for in vivo measurement of incorporated radioactivity; Empfehlungen zur Auswahl von Detektorsystemen zur Direktmessung der Koerperaktivitaet

    Energy Technology Data Exchange (ETDEWEB)

    Sahre, P. [Verein fuer Kernverfahrenstechnik und Analytik (VKTA) Rossendorf e.V., Dresden (Germany); Wahl, W. [GSF Forschungszentrum fuer Umwelt und Gesundheit, Neuherberg (Germany). Inst. fuer Strahlenschutz; Doerfel, H. [Forschungszentrum Karlsruhe GmbH (Germany)

    1997-12-01

    The paper presents and discusses a variety of aspects and objectives to be considered and explains the selection criteria to be applied with reference to the available detector systems. All available detector systems are shown and explained in a table. (orig./CB) [Deutsch] Zur Direktmessung der Koerperaktivitaet werden vorwiegend Halbleiter- und Szintillationsdetektoren verwendet. Bei Halbleiterdetektoren kommen der Regel HPGe-Detektoren und in Einzelfaellen auch Si(Li)-Detektoren zum Einsatz. Bei Szintillationsdetektoren werden hauptsaechlich NaI(TI)-Detektoren sowie NaI(TI)/CsI(TI)-Phoswich-Detektoren verwendet. Verschiedene Gesichtspunkte zur Auswahl von Detektorsystemen werden vorgestellt. (orig./SR)

  17. Treatment of osteochondritis dissecans of the femoral condyle with autologous bone grafts and matrix-supported autologous chondrocytes

    Science.gov (United States)

    Bruns, Juergen; Deuretzbacher, Georg; Ruether, Wolfgang; Fuerst, Martin; Niggemeyer, Oliver

    2009-01-01

    The objective of this study was to determine the clinical outcome of combined bone grafting and matrix-supported autologous chondrocyte transplantation in patients with osteochondritis dissecans of the knee. Between January 2003 and March 2005, 21 patients (mean age 29.33 years) with symptomatic osteochondritis dissecans (OCD) of the medial or lateral condyle (grade III or IV) of the knee underwent reconstruction of the joint surface by autologous bone grafts and matrix-supported autologous chondrocyte transplantation. Patients were followed up at three, six, 12 and 36 months to determine outcomes by clinical evaluation based on Lysholm score, IKDC and ICRS score. Clinical results showed a significant improvement of Lysholm-score and IKDC score. With respect to clinical assessment, 18 of 21 patients showed good or excellent results 36 months postoperatively. Our study suggests that treatment of OCD with autologous bone grafts and matrix-supported autologous chondrocytes is a possible alternative to osteochondral cylinder transfer or conventional ACT. PMID:19626325

  18. Improving diagnosis of appendicitis. Early autologous leukocyte scanning.

    Science.gov (United States)

    DeLaney, A R; Raviola, C A; Weber, P N; McDonald, P T; Navarro, D A; Jasko, I

    1989-10-01

    A prospective nonrandomized study investigating the accuracy and utility of autologous leukocyte scanning in the diagnosis of apendicitis was performed. One hundred patients in whom the clinical diagnosis of appendicitis was uncertain underwent indium 111 oxyquinoline labelling of autologous leukocytes and underwent scanning 2 hours following reinjection. Of 32 patients with proved appendicitis, three scans revealed normal results (false-negative rate, 0.09). Of 68 patients without appendicitis, three scans had positive results (false-positive rate, 0.03; sensitivity, 0.91; specificity, 0.97; predictive value of positive scan, 0.94; predictive value of negative scan, 0.96; and overall accuracy, 0.95). Scan results altered clinical decisions in 19 patients. In 13 cases, the scan produced images consistent with diagnoses other than appendicitis, expediting appropriate management. Early-imaging111 In oxyquinoline autologous leukocyte scanning is a practical and highly accurate adjunct for diagnosing appendicitis.

  19. [Treatment of relapsed Hodgkin lymphoma after autologous stem cell transplantation].

    Science.gov (United States)

    Illés, Árpád; Simon, Zsófia; Udvardy, Miklós; Magyari, Ferenc; Jóna, Ádám; Miltényi, Zsófia

    2017-08-01

    Approximately 10-30% of Hodgkin lymphoma patients relapses or experience refractory disease after first line treatment. Nowadays, autologous stem cell transplantation can successfully salvage half of these patients, median overall survival is only 2-2.5 years. Several prognostic factors determine success of autologous stem cell transplantation. Result of transplantation can be improved considering these factors and using consolidation treatment, if necessary. Patients who relapse after autologous transplantation had worse prognosis, treatment of this patient population is unmet clinical need. Several new treatment options became available in the recent years (brentuximab vedotin and immuncheckpoint inhibitors). These new treatment options offer more chance for cure in relapsed/refractory Hodgkin patients. Outcome of allogenic stem cell transplantation can be improved by using haploidentical donors. New therapeutic options will be discussed in this review. Orv Hetil. 2017; 158(34): 1338-1345.

  20. Therapeutic Potential of Autologous Stem Cell Transplantation for Cerebral Palsy

    Directory of Open Access Journals (Sweden)

    Chaitanya Purandare

    2012-01-01

    Full Text Available Background. Cerebral palsy (CP is a severe disabling disease with worldwide incidence being 2 to 3 per 1000 live births. CP was considered as a noncurable, nonreparative disorder, but stem cell therapy offers a potential treatment for CP. Objective. The present study evaluates the safety and efficacy of autologous bone-marrow-derived mononuclear cell (BMMNCs transplantation in CP patient. Material and Methods. In the present study, five infusions of autologous stem cells were injected intrathecally. Changes in neurological deficits and improvements in function were assessed using Gross Motor Function Classification System (GMFCS-E&R scale. Results. Significant motor, sensory, cognitive, and speech improvements were observed. Bowel and bladder control has been achieved. On the GMFCS-E&R level, the patient was promoted from grade III to I. Conclusion. In this study, we report that intrathecal infusion of autologous BMMNCs seems to be feasible, effective, and safe with encouraging functional outcome improvements in CP patient.

  1. Entwicklung einer Methodik zur Qualitätssicherung und Prozeßkontrolle von thermisch modifiziertem Holz mit Nah-Infrarotspektroskopie

    OpenAIRE

    Bächle, Helmut

    2010-01-01

    Thermisch modifizierte einheimische Hölzer bieten eine Alternative zur Verwendung von Holzschutzmitteln und Importhölzern. Die Arbeit untersucht die NIR-Spektroskopie als Methode zur Qualitätssicherung und Prozessüberwachung der thermischen Modifizierung. Neben der Anwendung im off-line Betrieb wurden Laborversuche zur in-line Anwendung durchgeführt. Die ergebnisse zeigen, dass die NIR-Spektroskopie zur Evaluierung der physikalisch-mechanischen Eigenschaften von fehlerfreien Proben eingesetzt...

  2. Prüfstand zur Bewertung und Optimierung von Geräten zur thermischen Unkrautbekämpfung

    Directory of Open Access Journals (Sweden)

    Detlef Stieg

    2014-08-01

    Full Text Available Der Bekämpfungserfolg beim Einsatz thermisch arbeitender Unkrautbekämpfungsgeräte kann in der Praxis starken Schwankungen unterliegen. Als Ursache wird vermutet, dass die Temperaturverteilung im Arbeitsbereich solcher Geräte nicht homogen ist, was bislang aufgrund fehlender Daten nicht nachgewiesen werden konnte. Um diese Hypothese zu belegen, wurde eine Methode zur systematischen Überprüfung von handgeschobenen Geräten für die thermische Unkrautbekämpfung entwickelt. Prüfkriterium ist die Verteilung der Temperaturverläufe im Arbeitsbereich der Geräte bei unterschiedlichen Arbeitsgeschwindigkeiten. Es konnte gezeigt werden, dass die Temperatur von der Arbeitsgeschwindigkeit abhängig ist und dass heterogene Temperaturverteilungen sehr gut nachgewiesen werden können.

  3. Autologous Mesenchymal Stem Cells in Chronic Stroke

    Directory of Open Access Journals (Sweden)

    Ashu Bhasin

    2011-12-01

    Full Text Available Background: Cell transplantation is a ‘hype and hope’ in the current scenario. It is in the early stage of development with promises to restore function in chronic diseases. Mesenchymal stem cell (MSC transplantation in stroke patients has shown significant improvement by reducing clinical and functional deficits. They are feasible and multipotent and have homing characteristics. This study evaluates the safety, feasibility and efficacy of autologous MSC transplantation in patients with chronic stroke using clinical scores and functional imaging (blood oxygen level-dependent and diffusion tensor imaging techniques. Methods: Twelve chronic stroke patients were recruited; inclusion criteria were stroke lasting 3 months to 1 year, motor strength of hand muscles of at least 2, and NIHSS of 4–15, and patients had to be conscious and able to comprehend. Fugl Meyer (FM, modified Barthel index (mBI, MRC, Ashworth tone grade scale scores and functional imaging scans were assessed at baseline, and after 8 and 24 weeks. Bone marrow was aspirated under aseptic conditions and expansion of MSC took 3 weeks with animal serum-free media (Stem Pro SFM. Six patients were administered a mean of 50–60 × 106 cells i.v. followed by 8 weeks of physiotherapy. Six patients served as controls. This was a non-randomized experimental controlled trial. Results: Clinical and radiological scanning was normal for the stem cell group patients. There was no mortality or cell-related adverse reaction. The laboratory tests on days 1, 3, 5 and 7 were also normal in the MSC group till the last follow-up. The FM and mBI showed a modest increase in the stem cell group compared to controls. There was an increased number of cluster activation of Brodmann areas BA 4 and BA 6 after stem cell infusion compared to controls, indicating neural plasticity. Conclusion: MSC therapy aiming to restore function in stroke is safe and feasible. Further randomized controlled trials are needed

  4. Ilse von zur Mühlen. Glanz und Elend. Mythos und Wirklichkeit der Herrenhäuser im Baltikum. Begleitpublikation zur gleichnahmigen Ausstellung des Ostpreußischen Landesmuseums und der Carl-Schirren-Gesellschaft e.V. / Anja Wilhelmi

    Index Scriptorium Estoniae

    Wilhelmi, Anja, 1966-

    2014-01-01

    Arvustus: Mühlen, Ilse von zur. Glanz und Elend. Mythos und Wirklichkeit der Herrenhäuser im Baltikum. Begleitpublikation zur gleichnahmigen Ausstellung des Ostpreußischen Landesmuseums und der Carl-Schirren-Gesellschaft e.V. (1. Dezember 2012 bis 14. April 2013), Lindenberg i. Allgäu 2012

  5. Resorbable screws for fixation of autologous bone grafts

    NARCIS (Netherlands)

    Raghoebar, GM; Liem, RSB; Bos, RRM; van der Wal, JE; Vissink, A

    The aim of this study was to evaluate the suitability of resorbable screws made of poly (D,L-lactide) acid (PDLLA) for fixation of autologous bone grafts related to graft regeneration and osseointegration of dental implants. In eight edentulous patients suffering from insufficient retention of their

  6. Management of Bone Gaps with Intramedullary Autologous Fibular ...

    African Journals Online (AJOL)

    ... 7 consecutive patients who presented with bone gaps that were managed with intramedullary non vascularised fibular strut graft. Method: Intramedulary Autologous fibular strut graft was used to breach the bone and the whole length augmented with cancellous graft and bridged with bone plate; external fixators or k wires.

  7. Experience with predonated autologous blood transfusion in open ...

    African Journals Online (AJOL)

    Objectives: To find out the practicability, the acceptability, the effectiveness and the safety level of pre-donated, autologous blood transfusion (ABT) in patients who underwent open prostatectomy. Study design: Prospective. Patients and methods: It was a prospective study carried out in Nigeria over a 5-year period.

  8. Review of autologous blood transfusion at the Kenyatta National ...

    African Journals Online (AJOL)

    Objective: This study was performed over a three- month period to establish the pattern of autologous blood transfusion with specific focus on age, sex, type of surgery, duration of hospital stay and religious beliefs. Design: Hospital based prospective study. Setting: The study was conducted at the Kenyatta National Hospital ...

  9. Surgical Patients\\' Knowledge and Acceptance of Autologous Blood ...

    African Journals Online (AJOL)

    Background: Homologous blood transfusion carries a well-documented array of risks especially in an HIV endemic environment like Nigeria. It is therefore imperative to consider other forms of restoring blood volume in surgical patients. Autologous blood transfusion (ABT) is one of the ways the problem of HIV transmission ...

  10. Ten years of preoperative autologous blood donation in Accra ...

    African Journals Online (AJOL)

    Background - Preoperative autologous blood donation (PABD) is utilized to circumvent the use of allogenic blood for various reasons. Objective - To describe the distribution in terms of demographic characteristic, trends in participation and result of screening test of the PABD programme of the Accra Area Blood Center from ...

  11. Autologous epidermal cell suspension: A promising treatment for chronic wounds.

    Science.gov (United States)

    Zhao, Hongliang; Chen, Yan; Zhang, Cuiping; Fu, Xiaobing

    2016-02-01

    Chronic wounds have become an increasing medical and economic problem of aging societies because they are difficult to manage. Skin grafting is an important treatment method for chronic wounds, which are refractory to conservative therapy. The technique involving epidermal cell suspensions was invented to enable the possibility of treating larger wounds with only a small piece of donor skin. Both uncultured and cultured autologous epidermal cell suspensions can be prepared and survive permanently on the wound bed. A systematic search was conducted of EMBASE, Cochrane Library, PubMed and web of science by using Boolean search terms, from the establishment of the database until May 31, 2014. The bibliographies of all retrieved articles in English were searched. The search terms were: (epithelial cell suspension OR keratinocyte suspension) and chronic and wound. From the included, 6 studies are descriptive interventions and discussed the use of autologous keratinocyte suspension to treat 61 patients' chronic wound. The various methods of preparation of epidermal cell suspension are described. The advantages and shortcomings of different carriers for epidermal cell suspensions are also summarised. Both uncultured and cultured autologous epidermal cell suspensions have been used to treat chronic wounds. Although the limitations of these studies include the small number of patient populations with chronic wounds and many important problems that remain to be solved, autologous epidermal cell suspension is a promising treatment for chronic wounds. Copyright © 2015 Tissue Viability Society. Published by Elsevier Ltd. All rights reserved.

  12. Bortezomib consolidation after autologous stem cell transplantation in multiple myeloma

    DEFF Research Database (Denmark)

    Mellqvist, Ulf-Henrik; Gimsing, Peter; Hjertner, Oyvind

    2013-01-01

    The Nordic Myeloma Study Group conducted an open randomized trial to compare bortezomib as consolidation therapy given after high-dose therapy and autologous stem cell transplantation (ASCT) with no consolidation in bortezomib-naive patients with newly diagnosed multiple myeloma. Overall, 370...

  13. Determinants of the Use of Autologous Blood in Elective General ...

    African Journals Online (AJOL)

    Objective: The study reports the 7 year experience of the authors with autologous blood transfusion in elective general surgery using the predeposit method. Material and Method: Patients aged 18 years and older, presenting for elective surgery and for whom blood donation was required were encouraged to predonate one ...

  14. Autologous hematopoietic stem cell transplantation for autoimmune diseases.

    NARCIS (Netherlands)

    Gratwohl, A.; Passweg, J.R.; Bocelli-Tyndall, C.; Fassas, A.; Laar, J.M. van; Farge, D.; Andolina, M.; Arnold, R.; Carreras, E.; Finke, J.; Kotter, I.; Kozak, T.; Lisukov, I.; Lowenberg, B.; Marmont, A.; Moore, J.; Saccardi, R.; Snowden, J.A.; Hoogen, F.H.J. van den; Wulffraat, N.M.; Zhao, X.; Tyndall, A.

    2005-01-01

    Experimental data and early phase I/II studies suggest that high-dose chemotherapy followed by autologous hematopoietic stem cell transplantation (HSCT) can arrest progression of severe autoimmune diseases. We have evaluated the toxicity and disease response in 473 patients with severe autoimmune

  15. The Influence of Barrier Membranes on Autologous Bone Grafts

    NARCIS (Netherlands)

    Gielkens, P. F. M.; Schortinghuis, J.; de Jong, J. R.; Paans, A. M. J.; Ruben, J. L.; Raghoebar, G. M.; Stegenga, B.; Bos, R. R. M.

    2008-01-01

    In implant dentistry, there is continuing debate regarding whether a barrier membrane should be applied to cover autologous bone grafts in jaw augmentation. A membrane would prevent graft remodeling with resorption and enhance graft incorporation. We hypothesized that membrane coverage does not

  16. Vaccination with apoptosis colorectal cancer cell pulsed autologous ...

    African Journals Online (AJOL)

    To investigate vaccination with apoptosis colorectal cancer (CRC) cell pulsed autologous dendritic cells (DCs) in advanced CRC, 14 patients with advanced colorectal cancer (CRC) were enrolled and treated with DCs vaccine to assess toxicity, tolerability, immune and clinical responses to the vaccine. No severe toxicity ...

  17. Do autologous blood and PRP injections effectively treat tennis elbow?

    Science.gov (United States)

    Widstrom, Luke; Slattengren, Andrew

    2016-09-01

    Both approaches reduce pain, but the improvement with platelet-rich plasma (PRP) is not clinically meaningful. Autologous blood injections (ABIs) are more effective than corticosteroid injections for reducing pain and disability in patients with tennis elbow in both the short and long term.

  18. Regeneration of Tissues and Organs Using Autologous Cells

    Energy Technology Data Exchange (ETDEWEB)

    Anthony Atala

    2010-04-28

    The Joint Commission for Health Care Organizations recently declared the shortage of transplantable organs and tissues a public health crisis. As such, there is about one death every 30 seconds due to organ failure. Complications and rejection are still significant albeit underappreciated problems. It is often overlooked that organ transplantation results in the patient being placed on an immune suppression regimen that will ultimate shorten their life span. Patients facing reconstruction often find that surgery is difficult or impossible due to the shortage of healthy autologous tissue. In many cases, autografting is a compromise between the condition and the cure that can result in substantial diminution of quality of life. The national cost of caring for persons who might benefit from engineered tissues or organs has reached $600 billion annually. Autologous tissue technologies have been developed as an alternative to transplantation or reconstructive surgery. Autologous tissues derived from the patient's own cells are capable of correcting numerous pathologies and injuries. The use of autologous cells eliminates the risks of rejection and immunological reactions, drastically reduces the time that patients must wait for lifesaving surgery, and negates the need for autologous tissue harvest, thereby eliminating the associated morbidities. In fact, the use of autologous tissues to create functional organs is one of the most important and groundbreaking steps ever taken in medicine. Although the basic premise of creating tissues in the laboratory has progressed dramatically, only a limited number of tissue developments have reached the patients to date. This is due, in part, to the several major technological challenges that require solutions. To that end, we have been in pursuit of more efficient ways to expand cells in vitro, methods to improve vascular support so that relevant volumes of engineered tissues can be grown, and constructs that can mimic the

  19. Independent and cooperative regulation of staphylopine biosynthesis and trafficking by Fur and Zur.

    Science.gov (United States)

    Fojcik, Clémentine; Arnoux, Pascal; Ouerdane, Laurent; Aigle, Marina; Alfonsi, Laura; Borezée-Durant, Elise

    2018-04-01

    Staphylococcus aureus expresses the Cnt system implicated in the active transport of trace metals by synthesizing (CntKLM) and exporting (CntE) staphylopine, a metallophore chelating metals and then taken up by an ABC-transporter (CntABCDF). This machinery is encoded in the cntKLMABCDFE operon, preceded by a non-coding region (PcntK) and containing an internal promoter region (PcntA). PcntK comprises a Fur box followed by a Zur box, a sRNA transcription start and a repeated region, while PcntA comprises a Fur box that overlaps a Zur box. We found that PcntK promoter activity is attenuated by the repeated sequence and strictly controlled by Fur or Zur binding to its respective target sequences. Interestingly, we discovered a cooperative regulation of the PcntA activity by both Fur and Zur binding to the Fur/Zur box, by identifying a tripartite complex with DNA. Repression of PcntA is less sensitive to metal concentration and therefore loosely repressed as compared to PcntK activity. Furthermore, the Cnt system is essential for the optimal import of zinc, thereby linking regulation and function of Cnt. Overall, our results highlight the need for fine and differential tuning of staphylopine biosynthesis and trafficking in order to efficiently respond to metal starvation and optimize metal recovery. © 2018 John Wiley & Sons Ltd.

  20. Use of containers with sterilizing filter in autologous serum eyedrops.

    Science.gov (United States)

    López-García, José S; García-Lozano, Isabel

    2012-11-01

    To assess the effect of the use of containers with an adapted sterilizing filter on the contamination of autologous serum eyedrops. Prospective, consecutive, comparative, and randomized study. Thirty patients with Sjögren syndrome. One hundred seventy-six autologous serum containers used in home therapy were studied; 48 of them included an adapted filter (Hyabak; Thea, Clermont-Ferrand, France), and the other 128 were conventional containers. Containers equipped with a filter were tested at 7, 14, 21, and 28 days of use, whereas conventional containers were studied after 7 days of use. In addition, testing for contamination was carried out in 14 conventional containers used during in-patient therapy every week for 4 weeks. In all cases, the preparation of the autologous serum was similar. Blood agar and chocolate agar were used as regular culture media for the microbiologic studies, whereas Sabouraud agar with chloramphenicol was the medium for fungal studies. Microbiologic contamination of containers with autologous serum eyedrops. Only one of the containers with an adapted sterilizing filter (2.1%) became contaminated with Staphylococcus epidermidis after 1 month of treatment, whereas the contamination rate among conventional containers reached 28.9% after 7 days of treatment. The most frequent germs found in the samples were coagulase-negative Staphylococcus (48.6%). With regard the containers used in the in-patient setting, 2 (14.3%) became contaminated after 2 weeks, 5 (35.7%) became contaminated after 3 weeks, and 5 (50%) became contaminated after 4 weeks, leaving 7 (50%) that did not become contaminated after 1 month of treatment. Using containers with an adapted filter significantly reduces the contamination rates in autologous serum eyedrops, thus extending the use of such container by the patients for up to 4 weeks with virtually no contamination risks. Copyright © 2012 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved.

  1. Treatment of severe osteochondral defects of the knee by combined autologous bone grafting and autologous chondrocyte implantation using fibrin gel

    NARCIS (Netherlands)

    Konst, Y.E.; Benink, R.J.; Veldstra, R.; van der Krieke, T.J.; Helder, M.N.; van Royen, B.J.

    2012-01-01

    Purpose: Severe symptomatic and unstable osteochondral defects of the knee are difficult to treat. A variety of surgical techniques have been developed. However, the optimal surgical technique is still controversial. We present a novel technique in which autologous bone grafting is combined with

  2. Radar sensor for detecting gas in fluids; Radarsensor zur Gasdetektion in Fluessigkeiten

    Energy Technology Data Exchange (ETDEWEB)

    Kunze, C. [MicAF Elektronik GmbH und Co. KG, Sensor-Systeme, Oelsnitz (Germany)

    1997-12-01

    Radar sensors are a relatively new and very promising instrument for monitoring operating conditions in plant and containers, in order to measure vibration and movement and for process automation and control. This is thanks to some very advantageous properties of the sensors based on mirowaves, compared to conventional sensors. (orig.) [Deutsch] Radar-Sensorik ist ein relativ neues und vielversprechendes Instrument zur Ueberwachung von Betriebszustaenden an Anlagen und Behaeltern, zur Schwingungs- und Bewegungsmessung sowie zur Prezessautomatisierung und -kontrolle. Dies ist vor allem einigen gegenueber konventionellen Sensoren vorteilhaften Eigenschaften der mirkowellenbasierten Sensorik zu verdanken. Die ersten Versuche an einzelnen Blasen zeigten eine breite Varianz an Zeitsignalen, die von einer Vielzahl von Einflussgroessen hervorgerufen wird. Bei Messungen an Zweiphasenstroemungen und der Auswertung der gewonnenen Doppler-Spektren zeigte sich, dass die Radar Sensorik fuer jeden Punkt der Flow Map reproduzierbare Ergebnisse liefert, und dass eine eindeutige Zuordnung der Stroemungsregimes zu den Spektren beim ersten Anlauf nicht moeglich war. (orig.)

  3. Optimum control strategy for phosphate elimination; Optimale Steuerstrategie zur Phosphatelimination

    Energy Technology Data Exchange (ETDEWEB)

    Heinrich, D.

    1997-12-31

    This study describes the most common phosphate elimination techniques. In the simplest cases, the chemicals are dosed according to previously established time curves. Nowadays, by contrast, dosage in proportion to quantity is largely preferred. Freight-proportional dosage will be more frequently employed in the future at plants for a population of more than 10000. Since investigations revealed that the results obtained using control systems are inferior to those obtained by means of automatic control systems, the latter are increasingly used. Even if PID control devices perform best, overshoot is not to be avoided with certainty because of the long dead time. According to the current state of knowledge, matched control and automatic control systems yield the best results. Further optimization can be achieved by driving them via a fuzzy-based system. (orig.) [Deutsch] In der vorliegenden Ausarbeitung wurden die gebraeuchlichsten Verfahren zur Phosphatelimination vorgestellt. In einfachsten Faellen wird die Dosierung nach festen Ganglinien vorgenommen. Sie wird heute weitgehend durch eine mengenproportionale Dosierung ersetzt. Frachtproportionale Dosierungen sind fuer Anlagen ueber 10.000 EW in Zukunft haeufiger zu finden. Nachdem die Untersuchungen gezeigt haben, dass Steuerungen nicht so gute Ergebnisse wie Reglungen ergaben, finden diese verstaerkt Anwendung. Auch wenn PID Regler das beste Ergebnis bringen, so kann mit Ihen wegen der langen Totzeiten ein Ueberschwingen nicht sicher vermieden werden. Nach heutiger Kenntnis erbringen aufeinander abgestimmte Steuer- und Reglungen die besten Ergebnisse. Werden sie ueber ein fuzzybasiertes System angesteuert, so ist eine weitere Optimierung gegeben. (orig.)

  4. Different methods of charge state assessment; Verschiedene Ansaetze zur Ladezustandsbestimmung

    Energy Technology Data Exchange (ETDEWEB)

    Rothert, M.; Willer, B.; Schmitz, C. [Institut fuer Solare Energieversorgungstechnik (ISET), Kassel (Germany); Bopp, G.; Sauer, D.U. [Fraunhofer-Institut fuer Solare Energiesysteme (ISE), Freiburg im Breisgau (Germany); Jossen, A. [Zentrum fuer Sonnenenergie- und Wasserstoff-Forschung Baden-Wuerttemberg (ZSW), Ulm (Germany)

    1999-07-01

    The state-of-charge of batteries is the central variable of state for the energy management of autonomous hybrid systems with a battery energy storage as well as for the battery management itself. Nevertheless, the determination of the state of charge is not solved precisely enough for many PV-applications. In the last years there have been new approaches and promising further developments especially with respect to the procedures based on models. These approaches are to be briefly explained and to be compared with one another in this article. In addition, a new approach regarding the determination of the state of charge will be shown by the ISET and first results regarding this approach with comparison to a balanced procedure will be presented. (orig.) [German] Der Ladezustand von Batterien ist die zentrale Zustandsgroesse sowohl fuer das Energiemanagement von autonomen Hybridsystemen mit einem Batteriespeicher als auch fuer die Batteriebetriebsfuehrung selber. Trotzdem ist die Bestimmung des Ladezustandes fuer viele PV-Anwendungen nicht ausreichend genau geloest. In neuerer Zeit hat es besonders bei den modellgestuetzten Verfahren neue Ansaetze und vielversprechende Weiterentwicklungen gegeben. Diese Ansaetze sollen in diesem Beitrag kurz erlaeutert und miteinander verglichen werden. Ausserdem wird ein neuer Ansatz zur Ladezustandsbestimmung vom ISET dargestellt und erste Ergebnisse dieses Ansatzes im Vergleich mit einem bilanzierenden Verfahren praesentiert. (orig.)

  5. 4. Lionel Gossman. "Remembering Hermynia Zur Mühlen: A Tribute"

    OpenAIRE

    Mühlen, Hermynia Zur

    2013-01-01

    ”Auch sie war unglücklich gewesen, kränklich und zart, aber welche Kraft sprach dennoch aus ihren Werken.” [She too had been unhappy, sickly, and frail, but what strength came through in her writings.]Hermynia Zur Mühlen on the poet Annette von Droste-Hülshoff in Unsere Töchter die Nazinen Hermynia Zur Mühlen was a contemporary, give or take a few years, of many well-known writers and artists from the last decades of the Austro-Hungarian Empire: Hugo von Hofmannsthal, Oskar Kokoschka, Karl Kr...

  6. Bio-artificial pleura using an autologous dermal fibroblast sheet

    Science.gov (United States)

    Kanzaki, Masato; Takagi, Ryo; Washio, Kaoru; Kokubo, Mami; Yamato, Masayuki

    2017-10-01

    Air leaks (ALs) are observed after pulmonary resections, and without proper treatment, can produce severe complications. AL prevention is a critical objective for managing patients after pulmonary resection. This study applied autologous dermal fibroblast sheets (DFS) to close ALs. For sealing ALs in a 44-year-old male human patient with multiple bullae, a 5 × 15-mm section of skin was surgically excised. From this skin specimen, primary dermal fibroblasts were isolated and cultured for 4 weeks to produce DFSs that were harvested after a 10-day culture. ALs were completely sealed using surgical placement of these autologous DFSs. DFS were found to be a durable long-term AL sealant, exhibiting requisite flexibility, elasticity, durability, biocompatibility, and usability, resulting reliable AL closure. DFS should prove to be an extremely useful tissue-engineered pleura substitute.

  7. Autologous Fat Transfer in a Patient with Lupus Erythematosus Profundus

    Directory of Open Access Journals (Sweden)

    Jimi Yoon

    2012-10-01

    Full Text Available Lupus erythematosus profundus, a form of chronic cutaneous lupus erythematosus, is a rare inflammatory disease involving in the lower dermis and subcutaneous tissues. It primarily affects the head, proximal upper arms, trunk, thighs, and presents as firm nodules, 1 to 3 cm in diameter. The overlying skin often becomes attached to the subcutaneous nodules and is drawn inward to produce deep, saucerized depressions. We present a rare case of lupus erythematosus profundus treated with autologous fat transfer.

  8. Herpes zoster after autologous hematopoietic stem cell transplantation

    Directory of Open Access Journals (Sweden)

    Kelli Borges dos Santos

    Full Text Available ABSTRACT Background: The autologous hematopoietic stem cell transplantation procedure involves immunosuppression of the patient. Thus, the patient has an elevated risk for several diseases, such as infections with the varicella-zoster virus. Prevention protocols have been proposed based on the use of acyclovir from the first day of conditioning, and maintaining this drug for 30-100 days after the procedure or for as much as one year. The objective of this work was to evaluate the incidence of herpes zoster after autologous transplantations related to the early suspension of acyclovir. Methods: A retrospective study was carried out based on the collection of data from 231 medical records of transplant patients in the Bone Marrow Transplant Unit of the teaching hospital of the Universidade Federal de Juiz de Fora in the period between 2004 and 2014. Results: Fourteen (6.1% patients had herpes zoster in the post-transplant period on average within six months of the procedure. Patients with multiple myeloma (64.3% were the most affected. There was a statistically significant difference in the age of the patients, with older individuals having a greater chance of developing the infection (p-value = 0.002. There were no significant differences for the other variables analyzed. Conclusion: The early suspension of acyclovir can be safe in patients who receive autologous hematopoietic stem cell transplants. However some groups may benefit from extended prophylaxis with acyclovir, particularly older patients and patients with multiple myeloma.

  9. Gallium-67 scanning and autologous transplantation for lymphoma

    Energy Technology Data Exchange (ETDEWEB)

    Mansberg, R.; Bautovich, G.J.; Forsyth, C.J.; Joshua, D.E.; Gibson, J. [Royal Prince Alfred Hospital, Sydney, NSW (Australia). Department of Nuclear Medicine and Haematology

    1998-06-01

    Full text: Gallium-67 ({sup 67}Ga) scanning in the malignant Iymphomas has been performed for over 20 years. Its major contributions are in staging and in detecting relapse, residual or progressive disease. Autologous bone marrow or peripheral blood stem cell transplantation is now an accepted therapy for refractory and relapsed Iymphoma. Between May 1991 and December 1995, 19 patients underwent autologous bone marrow or peripheral blood stem cell transplantation for Non-Hodgkin`s Iymphoma or Hodgkin`s disease. Five patients had high grade Non-Hodgkin`s Iymphoma with widespread disease and did not undergo {sup 67}Ga scanning. There was one transplant related death. Thirteen patients had {sup 67}Ga scanning pre- and post-transplantation. Six patients remained in clinical remission with no evidence of gallium avid active disease at a median of 11 months (range 3 to 19 months) post-transplant. Five of these patients had intermediate grade Non-Hodgkin`s Iymphoma and one had Hodgkin`s disease. The other seven patients all demonstrated evidence of active disease on {sup 67}Ga scanning and subsequent clinical relapse. In all patients shown to have {sup 67}Ga avid disease pre-transplant, {sup 67}Ga scanning post-transplant is useful in detecting relapse These results suggest that {sup 67}Ga avid disease pre-transplant, {sup 67}Ga scanning post-autologous transplantation as it is for conventional chemotherapy and radiotherapy

  10. Gallium-67 scanning and autologous transplantation for lymphoma

    International Nuclear Information System (INIS)

    Mansberg, R.; Bautovich, G.J.; Forsyth, C.J.; Joshua, D.E.; Gibson, J.

    1998-01-01

    Full text: Gallium-67 ( 67 Ga) scanning in the malignant Iymphomas has been performed for over 20 years. Its major contributions are in staging and in detecting relapse, residual or progressive disease. Autologous bone marrow or peripheral blood stem cell transplantation is now an accepted therapy for refractory and relapsed Iymphoma. Between May 1991 and December 1995, 19 patients underwent autologous bone marrow or peripheral blood stem cell transplantation for Non-Hodgkin's Iymphoma or Hodgkin's disease. Five patients had high grade Non-Hodgkin's Iymphoma with widespread disease and did not undergo 67 Ga scanning. There was one transplant related death. Thirteen patients had 67 Ga scanning pre- and post-transplantation. Six patients remained in clinical remission with no evidence of gallium avid active disease at a median of 11 months (range 3 to 19 months) post-transplant. Five of these patients had intermediate grade Non-Hodgkin's Iymphoma and one had Hodgkin's disease. The other seven patients all demonstrated evidence of active disease on 67 Ga scanning and subsequent clinical relapse. In all patients shown to have 67 Ga avid disease pre-transplant, 67 Ga scanning post-transplant is useful in detecting relapse These results suggest that 67 Ga avid disease pre-transplant, 67 Ga scanning post-autologous transplantation as it is for conventional chemotherapy and radiotherapy

  11. Tissue-engineered autologous grafts for facial bone reconstruction.

    Science.gov (United States)

    Bhumiratana, Sarindr; Bernhard, Jonathan C; Alfi, David M; Yeager, Keith; Eton, Ryan E; Bova, Jonathan; Shah, Forum; Gimble, Jeffrey M; Lopez, Mandi J; Eisig, Sidney B; Vunjak-Novakovic, Gordana

    2016-06-15

    Facial deformities require precise reconstruction of the appearance and function of the original tissue. The current standard of care-the use of bone harvested from another region in the body-has major limitations, including pain and comorbidities associated with surgery. We have engineered one of the most geometrically complex facial bones by using autologous stromal/stem cells, native bovine bone matrix, and a perfusion bioreactor for the growth and transport of living grafts, without bone morphogenetic proteins. The ramus-condyle unit, the most eminent load-bearing bone in the skull, was reconstructed using an image-guided personalized approach in skeletally mature Yucatán minipigs (human-scale preclinical model). We used clinically approved decellularized bovine trabecular bone as a scaffolding material and crafted it into an anatomically correct shape using image-guided micromilling to fit the defect. Autologous adipose-derived stromal/stem cells were seeded into the scaffold and cultured in perfusion for 3 weeks in a specialized bioreactor to form immature bone tissue. Six months after implantation, the engineered grafts maintained their anatomical structure, integrated with native tissues, and generated greater volume of new bone and greater vascular infiltration than either nonseeded anatomical scaffolds or untreated defects. This translational study demonstrates feasibility of facial bone reconstruction using autologous, anatomically shaped, living grafts formed in vitro, and presents a platform for personalized bone tissue engineering. Copyright © 2016, American Association for the Advancement of Science.

  12. Autologous Matrix-Induced Chondrogenesis in the Knee: A Review.

    Science.gov (United States)

    Lee, Yee Han Dave; Suzer, Ferzan; Thermann, Hajo

    2014-07-01

    Autologous matrix-induced chondrogenesis (AMIC) is a 1-step cartilage restoration technique that combines microfracture with the use of an exogenous scaffold. This matrix covers and mechanically stabilizes the clot. There have been an increasing number of studies performed related to the AMIC technique and an update of its use and results is warranted. Using the PubMed database, a literature search was performed using the terms "AMIC" or "Autologous Matrix Induced Chondrogenesis." A total of 19 basic science and clinical articles were identified. Ten studies that were published on the use of AMIC for knee chondral defects were identified and the results of 219 patients were analyzed. The improvements in Knee Injury and Osteoarthritis Outcome Score, International Knee Documentation Committee Subjective, Lysholm and Tegner scores at 2 years were comparable to the published results from autologous chondrocyte implantation (ACI) and matrix ACI techniques for cartilage repair. Our systematic review of the current state of the AMIC technique suggests that it is a promising 1-stage cartilage repair technique. The short-term clinical outcomes and magnetic resonance imaging results are comparable to other cell-based methods. Further studies with AMIC in randomized studies versus other repair techniques such as ACI are needed in the future.

  13. Eine selbstkonsistente Carleman Linearisierung zur Analyse von Oszillatoren

    Directory of Open Access Journals (Sweden)

    H. Weber

    2017-09-01

    Full Text Available Die Analyse nichtlinearer dynamischer Schaltungen ist bis heute eine herausfordernde Aufgabe, da nur selten analytische Lösungen angegeben werden können. Daher wurden eine Vielzahl von Methoden entwickelt, um eine qualitative oder quantitative Näherung für die Lösungen der Netzwerkgleichung zu erhalten. Oftmals wird beispielsweise eine Kleinsignalanalyse mit Hilfe einer Taylorreihe in einem Arbeitspunkt durchgeführt, die nach den Gliedern erster Ordnung abgebrochen wird. Allerdings ist diese Linearisierung nur in der Nähe des stabilen Arbeitspunktes für hyperbolische Systeme gültig. Besonders für die Analyse des dynamischen Verhaltens von Oszillatoren treten jedoch nicht-hyperbolische Systeme auf, sodass diese Methode nicht angewendet werden kann Mathis(2000. Carleman hat gezeigt, dass nichtlineare Differentialgleichungen mit polynomiellen Nichtlinearitäten in ein unendliches System von linearen Differentialgleichungen transformiert werden können Carleman(1932. Wird das unendlichdimensionale Gleichungssystem für numerische Zwecke abgebrochen, kann bei Oszillatoren der Übergang in eine stationäre Schwingung (Grenzzyklus nicht wiedergegeben werden.In diesem Beitrag wird eine selbstkonsistente Carleman Linearisierung zur Untersuchung von Oszillatoren vorgestellt, die auch dann anwendbar ist, wenn die Nichtlinearitäten keinen Polynomen entsprechen. Anstelle einer linearen Näherung um einen Arbeitspunkt, erfolgt mit Hilfe der Carleman Linearisierung eine Approximation auf einem vorgegebenen Gebiet. Da es jedoch mit der selbstkonsistenten Technik nicht möglich ist, das stationäre Verhalten von Oszillatoren zu beschreiben, wird die Berechnung einer Poincaré-Abbildung durchgeführt. Mit dieser ist eine anschließende Analyse des Oszillators möglich.

  14. Eine selbstkonsistente Carleman Linearisierung zur Analyse von Oszillatoren

    Science.gov (United States)

    Weber, Harry; Mathis, Wolfgang

    2017-09-01

    Die Analyse nichtlinearer dynamischer Schaltungen ist bis heute eine herausfordernde Aufgabe, da nur selten analytische Lösungen angegeben werden können. Daher wurden eine Vielzahl von Methoden entwickelt, um eine qualitative oder quantitative Näherung für die Lösungen der Netzwerkgleichung zu erhalten. Oftmals wird beispielsweise eine Kleinsignalanalyse mit Hilfe einer Taylorreihe in einem Arbeitspunkt durchgeführt, die nach den Gliedern erster Ordnung abgebrochen wird. Allerdings ist diese Linearisierung nur in der Nähe des stabilen Arbeitspunktes für hyperbolische Systeme gültig. Besonders für die Analyse des dynamischen Verhaltens von Oszillatoren treten jedoch nicht-hyperbolische Systeme auf, sodass diese Methode nicht angewendet werden kann Mathis (2000). Carleman hat gezeigt, dass nichtlineare Differentialgleichungen mit polynomiellen Nichtlinearitäten in ein unendliches System von linearen Differentialgleichungen transformiert werden können Carleman (1932). Wird das unendlichdimensionale Gleichungssystem für numerische Zwecke abgebrochen, kann bei Oszillatoren der Übergang in eine stationäre Schwingung (Grenzzyklus) nicht wiedergegeben werden. In diesem Beitrag wird eine selbstkonsistente Carleman Linearisierung zur Untersuchung von Oszillatoren vorgestellt, die auch dann anwendbar ist, wenn die Nichtlinearitäten keinen Polynomen entsprechen. Anstelle einer linearen Näherung um einen Arbeitspunkt, erfolgt mit Hilfe der Carleman Linearisierung eine Approximation auf einem vorgegebenen Gebiet. Da es jedoch mit der selbstkonsistenten Technik nicht möglich ist, das stationäre Verhalten von Oszillatoren zu beschreiben, wird die Berechnung einer Poincaré-Abbildung durchgeführt. Mit dieser ist eine anschließende Analyse des Oszillators möglich.

  15. Treatment of Adult Severe Traumatic Brain Injury Using Autologous Bone Marrow Mononuclear Cells

    Science.gov (United States)

    2014-12-01

    Our primary hypothesis is that bone marrow mononuclear cell (BMMNC) autologous transplantation after TBI is safe. Our secondary hypothesis is that...AD_________________ Award Number: W81XWH-11-1-0460 TITLE: TREATMENT OF ADULT SEVERE TRAUMATIC BRAIN INJURY USING AUTOLOGOUS BONE MARROW ... AUTOLOGOUS BONE MARROW MONONUCLEAR CELLS” 5a. CONTRACT NUMBER 5b. GRANT NUMBER: W81XWH-11-1-0460 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Charles S. Cox

  16. Untersuchungen zur Endoskopie juveniler Psittaziden unter besonderer Berücksichtigung der Geschlechtsorgane und ausgewählter biometrischer Befunde

    OpenAIRE

    Rühle, Dixie Michaela

    2006-01-01

    Die einschlägige Fachliteratur zur Taxonomie und Systematik der Vögel der Ordnung Psittaciformes, zur Inhalationsnarkose und zur Methodik der Geschlechtsbestimmung wurde referiert. Im Zeitraum von September 2004 bis Januar 2005 wurden insgesamt 340 Vögel aus der Ordnung Psittaciformes untersucht. Diese Vögel gehören insgesamt 37 Genera und 4 Tribi an. Bei allen Vögeln handelt es sich um Nachzuchten aus dem Loro Parque, Teneriffa, Spanien. Im genannten Zeitraum waren die Vögel zwischen sieben ...

  17. Bone graft substitutes for the treatment of traumatic fractures of the extremities [Knochenersatzmaterialien zur Behandlung von traumatischen Frakturen der Extremitäten

    Directory of Open Access Journals (Sweden)

    Hagen, Anja

    2012-06-01

    patient subgroup with high-grade open fractures (Gustilo-Anderson grade IIIB. Cost-effectiveness for BMP-2 versus standard care with autologous bone grafts as well as for other bone graft substitutes in fracture treatment has not been determined yet. Although there were some significant differences in favour of BMP-2, due to the overall poor quality of the studies the evidence can only be interpreted as suggestive for efficacy. In the case of CaP cements and bone marrow-based bone substitute materials, the evidence is only weakly suggestive for efficacy. From an overall economic perspective, the transferability of the results of the health economic evaluations to the current situation in Germany is limited.The current evidence is insufficient to evaluate entirely the use of different bone graft substitutes for fracture treatment. From a medical point of view, BMP-2 is a viable alternative for treatment of open fractures of the tibia, especially in cases where bone grafting is not possible. Autologous bone grafting is preferable comparing to the use of OP-1. Possible advantages of CaP cements and composites containing bone marrow over autogenous bone grafting should be taken into account in clinical decision making. The use of the hydroxyapatite material and allograft bone chips compared to autologous bone grafts cannot be recommended. From a health economic perspective, the use of BMP-2 in addition to standard care without bone grafting is recommended as cost-saving in patients with high-grade open fractures (Gustilo-Anderson grade IIIB. Based on the current evidence no further recommendations can be made regarding the use of bone graft substitutes for the treatment of fractures. To avoid legal implications, use of bone graft substitutes outside their approved indications should be avoided.[german] Bei der Behandlung von traumatischen Frakturen werden zusätzlich zur Standardtherapie und ggf. alternativ zu Knochentransplantaten zunehmend verschiedene

  18. [Heinz von zur Mühlen. Auf den Spuren einiger revlaer Firmen und Familien] / Paul Kaegbein

    Index Scriptorium Estoniae

    Kaegbein, Paul

    2007-01-01

    Arvustus: Heinz von zur Mühlen. Auf den Spuren einiger revlaer Firmen und Familien. In : Buch und Bildung im Baltikum. Münster : LIT, 2005, lk. 527-541. Pika tänava majade omanikest alates 17. sajandist - perekonnad Koch, Meyer, Kluge, Ströhm, Wassermann, Glehn, Eggers, Koppelson, Weiss. Nii loob autor pildi Tallinna "firmade ajaloost"

  19. Aktive Strategien zur Schutzzielverletzungerkennung durch eine kontrollierte Machtteilung in der Zugriffskontrollarchitektur

    OpenAIRE

    Abendroth, Jörg

    2004-01-01

    Zugangskontrolle und Intrusion Detection werden oft separat behandelt. Zur Erkennung von Schutzzielverletzungen wird hauptsächlich der Datenverkehr eines Netzwerkes ausgewertet - dies geschieht in einer passiven Weise. Aktive Strategien zum Erkennen von Angriffen sind nur durch neue Zugriffskontrollsysteme und kontrollierte Machtteilung möglich. In dem vorliegenden Beitrag wird eine neue Kategorisierung von Zugriffskontrollsysteme vorgestellt und insbesondere auf die kontrol...

  20. Einfalt der Vielfalt. Zur Heterogenität als neuer Norm der (Sexual-)Pädagogik

    OpenAIRE

    Dammer, Karl-Heinz

    2015-01-01

    Der Autor befasst sich mit der aktuellen Diskussion zur Schulentwicklung in Baden-Württemberg. Diese dreht sich um den Versuch, in den schulischen Lehrplan die sexuelle Vielfalt zum Thema zu machen. Dem Autor geht es dabei um die sexualpädagogischen Konsequenzen des Diversitätsdiskurses im Hinblick auf Heterogenität und ideologischen Implikationen von Normierung. (DIPF/JS)

  1. Folgebewertung im vorratsvermögen - Fallstudie zur Vorgehensweise nach IFSR und HGB

    DEFF Research Database (Denmark)

    Quick, Reiner; Warming-Rasmussen, Bent

    2013-01-01

    über die wichstigsten Normen zur Folgebeweriung nach IFSR und HGB sowie zu deren Auslegung. Es schliessen sich ein Fallbeispiel und ein Vorschlag zu dessen Lösung an, in dessen Mittelpunkt sinkende Zeitwerte, die Frage des für die Folgebewerunng relevanten Markts und das Zuschreibungsgebot stehen....

  2. Zur Internationalität der Pädagogischen Psychologie aus dem deutschsprachigen Bereich

    OpenAIRE

    Krampen, Günter; Schui, Gabriel

    2007-01-01

    Vor dem Hintergrund der Internationalisierungsdebatte der Psychologie in den deutschsprachigen Ländern wird der Internationalisierungsgrad der Pädagogischen Psychologie im Vergleich zur gesamten psychologischen Forschung aus dem deutschsprachigen Bereich unter zwei Perspektiven bibliometrisch untersucht: Zum ersten geht es inhaltlich um die Entwicklung der englischsprachigen Anteile der in PSYNDEX unter der Klassifikation "Pädagogische Psychologie" dokumentierten Literatur, zum zweiten um Ana...

  3. Zur Grundlegung eines Betriebspraktikums für Hauptschüler

    OpenAIRE

    Klebel, Hubert (Prof.)

    1984-01-01

    Zur Grundlegung eines Betriebspraktikums für Hauptschüler. - In: Das Betriebspraktikum für Hauptschüler / [Gesamtred.: Hubert Klebel ...]. - München : Studienkreis Schule-Wirtschaft Bayern, 1984. - S. 8-16. - (Schriftenreihe des BBW ; 13)

  4. Imidazol-Lipide : zur chemischen Implementierung eines Minimalen Chemotons als Modellsystem für Protozellen

    OpenAIRE

    Hellwig, Nils

    2011-01-01

    Zur chemischen Implementierung eines neuen Konzepts zu synthetischer Zellen - im Rahmen des EU-Projekts PACE - wurden zell-typische Reaktionssysteme (membranformendes-(1), metabolisches-(2) und genetisches-(3) Subsystem) umgesetzt und analysiert. Als zentrale Reaktionskomponenten dienten strukturunterschiedliche Imidazol-Lipide (Zugang über Festphasensynthese). Imidazol-Vesikel wurden über gezielte Selbstaggregationsprozesse hergestellt und mikroskopisch untersucht (Reaktionsconta...

  5. Regeneration of Cartilage in Human Knee Osteoarthritis with Autologous Adipose Tissue-Derived Stem Cells and Autologous Extracellular Matrix

    Directory of Open Access Journals (Sweden)

    Jaewoo Pak

    2016-08-01

    Full Text Available This clinical case series demonstrates that percutaneous injections of autologous adipose tissue-derived stem cells (ADSCs and homogenized extracellular matrix (ECM in the form of adipose stromal vascular fraction (SVF, along with hyaluronic acid (HA and platelet-rich plasma (PRP activated by calcium chloride, could regenerate cartilage-like tissue in human knee osteoarthritis (OA patients. Autologous lipoaspirates were obtained from adipose tissue of the abdominal origin. Afterward, the lipoaspirates were minced to homogenize the ECM. These homogenized lipoaspirates were then mixed with collagenase and incubated. The resulting mixture of ADSCs and ECM in the form of SVF was injected, along with HA and PRP activated by calcium chloride, into knees of three Korean patients with OA. The same affected knees were reinjected weekly with additional PRP activated by calcium chloride for 3 weeks. Pretreatment and post-treatment magnetic resonance imaging (MRI data, functional rating index, range of motion (ROM, and pain score data were then analyzed. All patients' MRI data showed cartilage-like tissue regeneration. Along with MRI evidence, the measured physical therapy outcomes in terms of ROM, subjective pain, and functional status were all improved. This study demonstrates that percutaneous injection of ADSCs with ECM contained in autologous adipose SVF, in conjunction with HA and PRP activated by calcium chloride, is a safe and potentially effective minimally invasive therapy for OA of human knees.

  6. Review: Holger Stoecker: Afrikawissenschaften in Berlin von 1919 bis 1945. Zur Geschichte und Topographie eines wissenschaftlichen Netzwerkes (2008 Buchbesprechung: Holger Stoecker: Afrikawissenschaften in Berlin von 1919 bis 1945. Zur Geschichte und Topographie eines wissenschaftlichen Netzwerkes

    Directory of Open Access Journals (Sweden)

    Katrin Bromber

    2009-05-01

    Full Text Available Review of the monograph: Holger Stoecker: Afrikawissenschaften in Berlin von 1919 bis 1945. Zur Geschichte und Topographie eines wissenschaftlichen Netzwerkes, Stuttgart: Franz Steiner Verlag 2008, ISBN 978-3-515-09161-9, 359 pages. Besprechung der Monographie: Holger Stoecker: Afrikawissenschaften in Berlin von 1919 bis 1945. Zur Geschichte und Topographie eines wissenschaftlichen Netzwerkes, Stuttgart: Franz Steiner Verlag 2008, ISBN 978-3-515-09161-9, 359 Seiten.

  7. SMILE - a simulation environment for the simulation of solar heating systems; Einsatz der Simulationsumgebung Smile zur Simulation solar unterstuetzter Heizsysteme

    Energy Technology Data Exchange (ETDEWEB)

    Jochum, P. [Technische Univ., Berlin (Germany). Inst. fuer Energietechnik

    1995-12-31

    Since 1990 Institut fuer Energietechnik, Technische Universitaet Berlin, has been developing a new system for the dynamic simulation of complex energy conversion processes to obtain a flexible program for the simulation of (almost) any general energy technology system configuration. Modules for efficiency analysis and optimization will be integrated to be able to use the program as a multivalent heating systems planning and design tool. (orig./HW) [Deutsch] Am Institut fuer Energietechnik der Technischen Universitaet Berlin wird seit 1990 an der Entwicklung eines neuen Systems zur dynamischen Simulation komplexer energiewandelnder Prozesse gearbeitet. Ziel der Arbeit ist die Erstellung eines moeglichst flexibel einsetzbaren Programms zur Simulation (fast) beliebiger Anlagenkonfigurationen aus der allgemeinen Energietechnik. Durch die Integration von Modulen zur Wirtschaftlichkeitsanalyse und zur Optimierung soll das entstehende Programm als Hilfsmittel fuer Planung und Auslegung von multivalenten Heizsystemen eingesetzt werden koennen. (orig./HW)

  8. Photovoltaic systems technology. Final report; Untersuchungen zur photovoltaischen Anlagentechnik. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Bosch, A.; Hoenes, H.P.; Honstetter, K.O.; Jossen, A.; Lehner, G.; Karl, H.; Saupe, G.; Zahir, A.

    1994-07-01

    wechselnden Einstrahlungsbedingungen wird untersucht. Pruefstaende fuer systematischen Lade-Entladebetrieb von Batterien werden aufgebaut. Die Belastungsprofile fuer die zu untersuchenden Batterien entsprechen einem photovoltaischen Anlagebetrieb. Die Untersuchungen geben ein Indiz dafuer, dass Bleibatterien mit Panzerplatten am besten fuer den photovoltaischen Betrieb geeignet sind. Dies gilt sowohl fuer ge- als auch fuer die verschlossene Bauweise. Messdaten legen den Schluss nahe, dass bei verschlossenen Bleibatterien die Gel- der Vliestechnologie ueberlegen ist. Das Verhalten der NiCd-Batterien war unter gleichen Bedingungen unbefriedigend. Zur verbesserten Betriebsfuehrung ist eine der wichtigsten Zustandsgroessen der Batterie ihr Ladezustand. Verschiedene Ansaetze dazu werden im Bericht aufgezeigt. Ein beachtenswerter Ansatz ist die Entwicklung eines selbstanpassenden Modells fuer die Batterie, das auf der Strombilanzierung und der Verlustbeschreibung basiert. Vorteil des Verfahrens ist, dass als Eingangsgroessen nur die Batteriespannung, der Batteriestrom und die Batterietemperatur benoetigt werden. (orig.)

  9. SPARC-System zur Behandlung der weiblichen Belastungsinkontinenz

    Directory of Open Access Journals (Sweden)

    Primus G

    2004-01-01

    nach 6 Monaten und auf 0,5; 2,38 g; 16,6 nach 12 Monaten. Die objektive komplette Heilrate entsprechend der Definition – keine Verwendung von Vorlagen, Vorlagentest 0–1 g, negativer Hustentest und keine urodynamisch nachweisbare Belastungsinkontinenz – nach 3, 6 und 12 Monaten beträgt 81,9 %, 82,8 % und 80 %. Subjektiv beurteilten sich 79,3 % nach 6 Monaten bzw. 74 % nach 12 Monaten als vollständig kontinent. Die Ergebnisse unserer prospektiven Studie untermauern die Sicherheit und Effektivität des SPARC-Verfahrens zur operativen Behandlung der weiblichen Belastungsinkontinenz.

  10. Häufig gestellte Fragen zur intrauterinen Insemination (IUI

    Directory of Open Access Journals (Sweden)

    Zech J

    2012-01-01

    Full Text Available Lediglich Fachärzte für Gynäkologie und Geburtshilfe dürfen laut österreichischem Fortpflanzungsmedizingesetz intrauterine Inseminationen durchführen. Nach Vorlage eines Notariatsakts ist es auch unverheirateten Paaren gestattet, diesen Eingriff vornehmen zu lassen. In Österreich ist die Durchführung einer heterologen Insemination (Samen von Dritten in dafür zugelassenen Krankenanstalten erlaubt. Die häufigste Indikation für eine IUI ist die idiopathische Infertilität, aber auch bei Ejakulationsproblemen und bei Pathologien an der Portio und der Cervix uteri sowie bei immunologischen Erkrankungen findet die IUI ihre Anwendung. Grundsätzlich ist bei Frauen mit einem regelmäßigen Zyklus eine hormonelle Stimulation nicht vonnöten. Zu beachten ist, dass eine hormonelle Stimulation zwar zur Steigerung der Erfolgschancen führt, jedoch häufig auch mit Komplikationen, wie beispielsweise Mehrlingsschwangerschaften oder einer Überstimulation, verbunden ist. Wichtig bei der Samenaufbereitung für eine IUI ist die Trennung der Spermien von Seminalflüssigkeit, Bakterien, Leukozyten und Zelldebris sowie die Gewinnung von 2–5 Millionen gut beweglichen Spermien. Es gibt unterschiedliche Aufbereitungsmethoden wie die Swim-up-Technik oder die Dichte-Gradienten-Zentrifugation, welche jedoch für die Aufreinigung der Spermien Zentrifugationsschritte benötigen, was eine mechanische Belastung für die Spermien darstellt. Eine neue, sehr einfache, schonende und kostengünstige Methode bietet der Zech-Selector®, welcher als einzige Methode in der Lage ist, auf einfache Weise nahezu alle Spermien mit DNAStrangbrüchen zu eliminieren. Ein weiterer wichtiger Bestandteil für eine erfolgreiche IUI ist die Wahl des Katheters. Dieser muss für die IUI zugelassen sein und darf beim Einführen keine Schleimhautverletzungen herbeiführen. Des Weiteren sollten nicht mehr als 0,5 ml Flüssigkeit in das Cavum uteri eingespritzt werden. Optimal ist es, die

  11. Entwicklung und Validierung einer elektrochemischen Methode zur Differenzierung von Weizenproben und elektrochemische Unterschungen an Möhren

    OpenAIRE

    Roose, Marco; Desche, Marco [früherer Name

    2004-01-01

    Eine Methode zur Differenzierung von Weizenproben (Triticum aestivum L.) mittels elektrochemischer Parameter wurde entwickelt und validiert. Die Methode wurde auf die wichtigsten Einfluss- und Verfahrensgrößen hin untersucht. Als eine Voraussetzung zur Validierung wurden alle Laborprozesse in normativen Dokumenten beschrieben. Dies ist zum ersten Mal überhaupt für die Methode in diesem Umfang durchgeführt worden. Mit der Methode wurden Messungen durchgeführt, um Weizenproben au...

  12. Autologous hematopoietic stem cell transplantation in classical Hodgkin's lymphoma

    Directory of Open Access Journals (Sweden)

    Afonso José Pereira Cortez

    2011-02-01

    Full Text Available BACKGROUND: Hodgkin's lymphoma has high rates of cure, but in 15% to 20% of general patients and between 35% and 40% of those in advanced stages, the disease will progress or will relapse after initial treatment. For this group, hematopoietic stem cell transplantation is considered one option of salvage therapy. OBJECTIVES: To evaluate a group of 106 patients with Hodgkin's lymphoma, who suffered relapse or who were refractory to treatment, submitted to autologous hematopoietic stem cell transplantation in a single transplant center. METHODS: A retrospective study was performed with data collected from patient charts. The analysis involved 106 classical Hodgkin's lymphoma patients who were consecutively submitted to high-dose chemotherapy followed by autologous transplants in a single institution from April 1993 to December 2006. RESULTS: The overall survival rates of this population at five and ten years were 86% and 70%, respectively. The disease-free survival was approximately 60% at five years. Four patients died of procedure-related causes but relapse of classical Hodgkin's lymphoma after transplant was the most frequent cause of death. Univariate analysis shows that sensitivity to pre-transplant treatment and hemoglobin < 10 g/dL at diagnosis had an impact on patient survival. Unlike other studies, B-type symptoms did not seem to affect overall survival. Lactic dehydrogenase and serum albumin concentrations analyzed at diagnosis did not influence patient survival either. CONCLUSION: Autologous hematopoietic stem cell transplantation is an effective treatment strategy for early and late relapse in classical Hodgkin's lymphoma for cases that were responsive to pre-transplant chemotherapy. Refractory to treatment is a sign of worse prognosis. Additionally, a hemoglobin concentration below 10 g/dL at diagnosis of Hodgkin's lymphoma has a negative impact on the survival of patients after transplant. As far as we know this relationship has not

  13. Bacillus licheniformis Contains Two More PerR-Like Proteins in Addition to PerR, Fur, and Zur Orthologues.

    Directory of Open Access Journals (Sweden)

    Jung-Hoon Kim

    Full Text Available The ferric uptake regulator (Fur family proteins include sensors of Fe (Fur, Zn (Zur, and peroxide (PerR. Among Fur family proteins, Fur and Zur are ubiquitous in most prokaryotic organisms, whereas PerR exists mainly in Gram positive bacteria as a functional homologue of OxyR. Gram positive bacteria such as Bacillus subtilis, Listeria monocytogenes and Staphylococcus aureus encode three Fur family proteins: Fur, Zur, and PerR. In this study, we identified five Fur family proteins from B. licheniformis: two novel PerR-like proteins (BL00690 and BL00950 in addition to Fur (BL05249, Zur (BL03703, and PerR (BL00075 homologues. Our data indicate that all of the five B. licheniformis Fur homologues contain a structural Zn2+ site composed of four cysteine residues like many other Fur family proteins. Furthermore, we provide evidence that the PerR-like proteins (BL00690 and BL00950 as well as PerRBL (BL00075, but not FurBL (BL05249 and ZurBL (BL03703, can sense H2O2 by histidine oxidation with different sensitivity. We also show that PerR2 (BL00690 has a PerR-like repressor activity for PerR-regulated genes in vivo. Taken together, our results suggest that B. licheniformis contains three PerR subfamily proteins which can sense H2O2 by histidine oxidation not by cysteine oxidation, in addition to Fur and Zur.

  14. Bacillus licheniformis Contains Two More PerR-Like Proteins in Addition to PerR, Fur, and Zur Orthologues

    Science.gov (United States)

    Ju, Shin-Yeong; Yang, Yoon-Mo; Ryu, Su-Hyun; Kwon, Yumi; Won, Young-Bin; Lee, Yeh-Eun; Youn, Hwan; Lee, Jin-Won

    2016-01-01

    The ferric uptake regulator (Fur) family proteins include sensors of Fe (Fur), Zn (Zur), and peroxide (PerR). Among Fur family proteins, Fur and Zur are ubiquitous in most prokaryotic organisms, whereas PerR exists mainly in Gram positive bacteria as a functional homologue of OxyR. Gram positive bacteria such as Bacillus subtilis, Listeria monocytogenes and Staphylococcus aureus encode three Fur family proteins: Fur, Zur, and PerR. In this study, we identified five Fur family proteins from B. licheniformis: two novel PerR-like proteins (BL00690 and BL00950) in addition to Fur (BL05249), Zur (BL03703), and PerR (BL00075) homologues. Our data indicate that all of the five B. licheniformis Fur homologues contain a structural Zn2+ site composed of four cysteine residues like many other Fur family proteins. Furthermore, we provide evidence that the PerR-like proteins (BL00690 and BL00950) as well as PerRBL (BL00075), but not FurBL (BL05249) and ZurBL (BL03703), can sense H2O2 by histidine oxidation with different sensitivity. We also show that PerR2 (BL00690) has a PerR-like repressor activity for PerR-regulated genes in vivo. Taken together, our results suggest that B. licheniformis contains three PerR subfamily proteins which can sense H2O2 by histidine oxidation not by cysteine oxidation, in addition to Fur and Zur. PMID:27176811

  15. Autologous fat grafting for cosmetic enhancement of the perioral region.

    Science.gov (United States)

    Glasgold, Mark; Lam, Samuel M; Glasgold, Robert

    2007-11-01

    The role of volume loss in the progression of facial aging is widely accepted as an important cause. The aging appearance of the perioral region and lower face is significantly affected by this volume loss, which contributes to the development of labiomental folds, the loss of definition of the jawline, and worsening of skin texture, among other manifestations. Autologous fat transfer can effectively replace this lost volume and contribute to any facial rejuvenation plan. Fat can replace larger volumes than off-the-shelf fillers and provides a potentially permanent solution.

  16. Facial fat necrosis following autologous fat transfer and its management

    Directory of Open Access Journals (Sweden)

    Sweta Rai

    2014-01-01

    Full Text Available Autologous fat transfer (AFT is an increasingly popular cosmetic procedure practiced by dermatologic surgeons worldwide. As this is an office based procedure performed under local or tumescent anaesthesia with fat transferred within the same individual and limited associated down time its is considered relatively safe and risk free in the cosmetic surgery arena. We describe a case of AFT related fat necrosis causing significant facial dysmorphia and psychosocial distress. We also discuss the benefits and risks of AFT highlighting common causes of fat graft failure.

  17. LOCAL CORTICOSTEROID VS. AUTOLOGOUS BLOOD FOR PLANTAR FASCIITIS

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    Syam Sunder B

    2017-01-01

    Full Text Available BACKGROUND Plantar fasciitis is the most common cause of heel pain for which professional care is sought. Initially thought of as an inflammatory process, plantar fasciitis is a disorder of degenerative changes in the fascia and maybe more accurately termed plantar fasciosis. Traditional therapeutic efforts have been directed at decreasing the presumed inflammation. These treatments include icing, Nonsteroidal Anti-inflammatory Drugs (NSAIDs, rest and activity modification, corticosteroids, botulinum toxin type A, splinting, shoe modifications and orthosis. Other treatment techniques have been directed at resolving the degeneration caused by the disease process. In general, these techniques are designed to create an acute inflammatory reaction with the goal of restarting the healing process. These techniques include autologous blood injection, Platelet-Rich Plasma (PRP injection, nitroglycerin patches, Extracorporeal Shock Wave Therapy (ESWT and surgical procedures. Recently, research has focused on regenerative therapies with high expectations of success. The use of autologous growth factors is thought to heal through collagen regeneration and the stimulation of a well-ordered angiogenesis. These growth factors are administered in the form of autologous whole blood or Platelet-Rich Plasma (PRP. Platelets can be isolated using simple cell-separating systems. The degranulation of the alpha granules in the platelets releases many different growth factors that play a role in tissue regeneration processes. Platelet-derived growth factor, transforming growth factor-P, vascular-derived endothelial growth factor, epithelial growth factor, hepatocyte growth factor and insulin-like growth factor are examples of such growth factors. Injections with autologous growth factors are becoming common in clinical practice. The present study was an attempt to compare the efficacy of autologous blood injection in plantar fasciitis by comparing it with the local

  18. Autologous Fat Transfer: An Aesthetic and Functional Refinement for Parotidectomy

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    Pierre G. Vico

    2014-01-01

    Full Text Available Parotidectomy is a surgical procedure associated to functional (Frey’s syndrome as well as aesthetic (facial asymmetry complications that can be very disturbing for the patient. Several procedures have been described to primarily avoid or secondarily reconstruct the facial defect and treat the neurological iatrogenic syndrome. Autologous fat transfer was primarily used in 10 cases to avoid such complications. It is an easy technique widely used in cosmetic and reconstructive surgery. This technique gives very satisfying long-term results on the cosmetic as well as on the physiological point of view.

  19. Autologous 111In-oxine-labeled granulocytes in Yersinia infections

    International Nuclear Information System (INIS)

    Becker, W.; Boerner, W.; Fischbach, W.

    1985-01-01

    Autologous 111 In-oxine-labeled granulocytes have proved to be valuable for the localization of inflammatory bowel diseases, especially Crohn's disease and ulcerative colitis. Other rare inflammatory bowel diseases also yield positive 111 In scans. One case of Yersinia infection of the terminal ileum (Yersinia enterocolitica) showing an accumulation of 111 In-oxine-labeled granulocytes 0.5, 4, and 24 h after the reinjection of the labeled cells is described. The 4-day fecal excretion of 111 In-oxine granulocytes showed a slight inflammatory activity of the terminal ileum. One negative scan is reported in a cotrimoxazole-treated patient with Yersinia infection. (orig.)

  20. Pulmonary heart valve replacement using stabilized acellular xenogeneic scaffolds; effects of seeding with autologous stem cells

    Directory of Open Access Journals (Sweden)

    Harpa Marius Mihai

    2015-12-01

    Full Text Available Background: We hypothesized that an ideal heart valve replacement would be acellular valve root scaffolds seeded with autologous stem cells. To test this hypothesis, we prepared porcine acellular pulmonary valves, seeded them with autologous adipose derived stem cells (ADSCs and implanted them in sheep and compared them to acellular valves.

  1. Impulse für Entwicklungsprozesse zur inklusiven Schule aus der interkulturellen Bildungspraxis: inwiefern können internationale biografische Erfahrungen von Lehrer(inne)n und Schüler(inne)n zur Integrations- und zur Enkulturationsfunktion von Schule beitragen?

    OpenAIRE

    Egbers, Julia; Hüllbrock, Nadine

    2017-01-01

    Die inklusive Schule entwickelt sich aus komplexen Veränderungsprozessen, die Vielfalt auf unterschiedlichste Weisen abbilden. Ein Aspekt ist die kulturelle Diversität. Sie steht im Fokus dieses Beitrags und wird unter folgender Fragestellung betrachtet: Inwiefern können internationale biografische Erfahrungen von Lehrer(inne)n und Schüler(inne)n zur Integrations- und zur Enkulturationsfunktion von Schule beitragen? Ziel der folgenden Darstellung ist es, auf der Basis von zwei Projektbeispiel...

  2. [Philipp Herzog: Sozialistische Völkerfreundschaft nationaler Widerstand oder harmloser Zeitvertieb? Zur politischen Funktion der Volkskunst im sowjetischen Estland] / Karsten Brüggemann

    Index Scriptorium Estoniae

    Brüggemann, Karsten, 1965-

    2014-01-01

    Arvustus: Herzog, Philipp. Sozialistische Völkerfreundschaft, nationaler Widerstand oder harmloser Zeitvertreib? : zur politischen Funktion der Volkskunst im sowjetischen Estland. Stuttgart : Ibidem-Verlag, 2012

  3. Models for settlement prediction of structures in opencast dumps; Modelle zur Setzungsprognose von Bauwerken auf Tagebaukippen

    Energy Technology Data Exchange (ETDEWEB)

    Birle, E.; Vogt, S. [Technische Univ. Muenchen (Germany). Zentrum Geotechnik

    2016-05-01

    For construction projects at opencast dumps e.g. for planning of traffic routes or individual buildings a prognosis of dump subsicence after the establishment of the structures is required. It should be noted that on dump surfaces time-dependent subsidence are observed which can change by construction activities at the dump surface. As tools for describing the dump subsidence phenomenological models that have been developed based on the evaluation of dump characteristic subsidence and allow a one-dimensional analysis of creep deformation and visco-elastoplastic material models within the finite element method for analyzing complex geometric boundary conditions. [German] Fuer Baumassnahmen auf Tagebaukippen beispielsweise zur Planung von Verkehrswegen oder einzelnen Bauwerken ist eine Prognose der Kippensetzungen nach Errichtung der Bauwerke erforderlich. Dabei ist zu beachten, dass auf Kippenoberflaechen ausgepraegte zeitabhaengige Setzungen beobachtet werden, die sich durch Baumassnahmen an der Kippenoberflaeche aendern koennen. Als Werkzeuge zur Beschreibung der Kippensetzungen stehen prinzipiell phaenomenologische Modelle, die auf Basis der Auswertung von Kippeneigensetzungen entwickelt wurden und die eine ein-dimensionale Analyse der Kriechverformungen zulassen sowie visko-elastoplastische Stoffmodelle im Rahmen der FiniteElemente- Methode zur Analyse komplexer geometrischer Randbedingungen zur Verfuegung. In Abhaengigkeit vom Randwertproblem ist zu entscheiden, welches der Modelle zur Beschreibung der Kippensetzungen heranzuziehen ist. Mit phaenomenologischen Kriechmodellen und einer ein-dimensionalen Analyse der Verformungen koennen die sich nach Ende der Verkippung einstellenden Kippensetzungen in der Regel genuegend genau beschrieben werden, sofern an der Kippenoberflaeche keine signifikanten Spannungsaenderungen infolge von Baumassnahmen eingetragen werden. Kommt es dagegen zu Spannungsaenderungen, beispielsweise durch die Errichtung eines hohen

  4. Autologous hematopoietic stem cells for refractory Crohn's disease.

    Science.gov (United States)

    DiNicola, C A; Zand, A; Hommes, D W

    2017-05-01

    Autologous hematopoietic stem cells are gaining ground as an effective and safe treatment for treating severe refractory Crohn's disease (CD). Autologous hematopoietic stem cell therapy (AHSCT) induces resetting of the immune system by de novo regeneration of T-cell repertoire and repopulation of epithelial cells by bone-marrow derived cells to help patients achieve clinical and endoscopic remission. Areas covered: Herein, the authors discuss the use of AHSCT in treating patients with CD. Improvements in disease activity have been seen in patients with severe autoimmune disease and patients with severe CD who underwent AHSCT for a concomitant malignant hematological disease. Clinical and endoscopic remission has been achieved in patients treated with AHSCT for CD. The only randomized trial published to date, the ASTIC Trial, did not support further use of AHSCT to treat CD. Yet, critics of this trial have deemed AHSCT as a promising treatment for severe refractory CD. Expert opinion: Even with the promising evidence presented for HSCT for refractory CD, protocols need to be refined through the collaboration of GI and hemato-oncology professionals. The goal is to incorporate safe AHSCT and restore tolerance by delivering an effective immune 'cease fire' as a treatment option for severe refractory CD.

  5. Subcutaneous autologous serum therapy in chronic spontaneous urticaria

    Directory of Open Access Journals (Sweden)

    Kiran Vasant Godse

    2017-01-01

    Full Text Available Background: There is a felt need for trying newer therapeutic modalities in patients with chronic spontaneous urticaria, especially in the subset of patients classified as non-responders to antihistamines. Autologous serum therapy is an upcoming modality of treatment, and we decided to study its efficacy by subcutaneous route. Aims: To evaluate the effectiveness of subcutaneous autologous serum therapy (AST in CSU. Methods: This was a single blind, placebo-controlled parallel group, randomized, controlled study. Twenty-four patients with CSU (11M: 13 F were given subcutaneous AST and seventeen patients (7 M: 10F patients were given subcutaneous injection normal saline (placebo, along with levocetirizine in an on-demand basis in both groups. Results: Urticaria activity score (UAS came down from 35.74 to 7 at the end of 9 weeks and the patients' requirement of antihistamines also reduced remarkably from 5.8 to 1.7 per week in the serum group. Sub-cutaneous saline group did not show statistically significant fall in UAS. Saline group showed UAS 32.8 at zero week to 22.1 at the end of 9 weeks. DLQI showed significant fall in serum group, from 14.26 to 4 at the end of 9 weeks. Conclusion: Subcutaneous autoserum therapy is effective in treatment of CSU.

  6. MR imaging of osteochondral grafts and autologous chondrocyte implantation

    Energy Technology Data Exchange (ETDEWEB)

    Trattnig, S. [Medical University of Vienna, MR Centre of Excellence, Department of Radiology, Vienna (Austria); University Hospital of Vienna, MR-Center, Department of Radiology, Vienna (Austria); Millington, S.A. [Medical University of Vienna, MR Centre of Excellence, Department of Radiology, Vienna (Austria); Medical University of Vienna, Department of Trauma Surgery, Center for Joints and Cartilage, Vienna (Austria); Szomolanyi, P. [Medical University of Vienna, MR Centre of Excellence, Department of Radiology, Vienna (Austria); Marlovits, S. [Medical University of Vienna, Department of Trauma Surgery, Center for Joints and Cartilage, Vienna (Austria)

    2007-01-15

    Surgical articular cartilage repair therapies for cartilage defects such as osteochondral autograft transfer, autologous chondrocyte implantation (ACI) or matrix associated autologous chondrocyte transplantation (MACT) are becoming more common. MRI has become the method of choice for non-invasive follow-up of patients after cartilage repair surgery. It should be performed with cartilage sensitive sequences, including fat-suppressed proton density-weighted T2 fast spin-echo (PD/T2-FSE) and three-dimensional gradient-echo (3D GRE) sequences, which provide good signal-to-noise and contrast-to-noise ratios. A thorough magnetic resonance (MR)-based assessment of cartilage repair tissue includes evaluations of defect filling, the surface and structure of repair tissue, the signal intensity of repair tissue and the subchondral bone status. Furthermore, in osteochondral autografts surface congruity, osseous incorporation and the donor site should be assessed. High spatial resolution is mandatory and can be achieved either by using a surface coil with a 1.5-T scanner or with a knee coil at 3 T; it is particularly important for assessing graft morphology and integration. Moreover, MR imaging facilitates assessment of complications including periosteal hypertrophy, delamination, adhesions, surface incongruence and reactive changes such as effusions and synovitis. Ongoing developments include isotropic 3D sequences, for improved morphological analysis, and in vivo biochemical imaging such as dGEMRIC, T2 mapping and diffusion-weighted imaging, which make functional analysis of cartilage possible. (orig.)

  7. Alteration of Skin Properties with Autologous Dermal Fibroblasts

    Directory of Open Access Journals (Sweden)

    Rajesh L. Thangapazham

    2014-05-01

    Full Text Available Dermal fibroblasts are mesenchymal cells found between the skin epidermis and subcutaneous tissue. They are primarily responsible for synthesizing collagen and glycosaminoglycans; components of extracellular matrix supporting the structural integrity of the skin. Dermal fibroblasts play a pivotal role in cutaneous wound healing and skin repair. Preclinical studies suggest wider applications of dermal fibroblasts ranging from skin based indications to non-skin tissue regeneration in tendon repair. One clinical application for autologous dermal fibroblasts has been approved by the Food and Drug Administration (FDA while others are in preclinical development or various stages of regulatory approval. In this context, we outline the role of fibroblasts in wound healing and discuss recent advances and the current development pipeline for cellular therapies using autologous dermal fibroblasts. The microanatomic and phenotypic differences of fibroblasts occupying particular locations within the skin are reviewed, emphasizing the therapeutic relevance of attributes exhibited by subpopulations of fibroblasts. Special focus is provided to fibroblast characteristics that define regional differences in skin, including the thick and hairless skin of the palms and soles as compared to hair-bearing skin. This regional specificity and functional identity of fibroblasts provides another platform for developing regional skin applications such as the induction of hair follicles in bald scalp or alteration of the phenotype of stump skin in amputees to better support their prosthetic devices.

  8. Autologous Bone Marrow Mononuclear Cells Intrathecal Transplantation in Chronic Stroke

    Directory of Open Access Journals (Sweden)

    Alok Sharma

    2014-01-01

    Full Text Available Cell therapy is being widely explored in the management of stroke and has demonstrated great potential. It has been shown to assist in the remodeling of the central nervous system by inducing neurorestorative effect through the process of angiogenesis, neurogenesis, and reduction of glial scar formation. In this study, the effect of intrathecal administration of autologous bone marrow mononuclear cells (BMMNCs is analyzed on the recovery process of patients with chronic stroke. 24 patients diagnosed with chronic stroke were administered cell therapy, followed by multidisciplinary neurorehabilitation. They were assessed on functional independence measure (FIM objectively, along with assessment of standing and walking balance, ambulation, and hand functions. Out of 24 patients, 12 improved in ambulation, 10 in hand functions, 6 in standing balance, and 9 in walking balance. Further factor analysis was done. Patients of the younger groups showed higher percentage of improvement in all the areas. Patients who underwent cell therapy within 2 years after the stroke showed better changes. Ischemic type of stroke had better recovery than the hemorrhagic stroke. This study demonstrates the potential of autologous BMMNCs intrathecal transplantation in improving the prognosis of functional recovery in chronic stage of stroke. Further clinical trials are recommended. This trial is registered with NCT02065778.

  9. Autologous Blood Injection and Wrist Immobilisation for Chronic Lateral Epicondylitis

    Directory of Open Access Journals (Sweden)

    Nicola Massy-Westropp

    2012-01-01

    Full Text Available Purpose. This study explored the effect of autologous blood injection (with ultrasound guidance to the elbows of patients who had radiologically assessed degeneration of the origin of extensor carpi radialis brevis and failed cortisone injection/s to the lateral epicondylitis. Methods. This prospective longitudinal series involved preinjection assessment of pain, grip strength, and function, using the patient-rated tennis elbow evaluation. Patients were injected with blood from the contralateral limb and then wore a customised wrist support for five days, after which they commenced a stretching, strengthening, and massage programme with an occupational therapist. These patients were assessed after six months and then finally between 18 months and five years after injection, using the patient-rated tennis elbow evaluation. Results. Thirty-eight of 40 patients completed the study, showing significant improvement in pain; the worst pain decreased by two to five points out of a 10-point visual analogue for pain. Self-perceived function improved by 11–25 points out of 100. Women showed significant increase in grip, but men did not. Conclusions. Autologous blood injection improved pain and function in a worker’s compensation cohort of patients with chronic lateral epicondylitis, who had not had relief with cortisone injection.

  10. Untersuchungen zur Eignung des zweidimensionalen Klinostaten und der Random Positioning Machine zur Simulation von Mikrogravitation für gravitaktische Protozoen

    OpenAIRE

    Hoppe, Sascha

    2013-01-01

    Ziel dieser Arbeit ist es herauszufinden, ob man mit einem 2D oder 3D-Klinostaten oder einer Random Positioning Machine simulierte Mikrogravitation für die gravitaktischen Protozoen Paramecium biaurelia und Euglena gracilis erreichen kann. Glasbeads wurden zur Demonstration der physikalischen Vorgänge bei Rotation eingesetzt. Im 2D-Klinostatenmodus zeigen sie die zu erwartenden Kreisbahnen gemäß der Hypothese. In den 3D-Modi wurde dagegen eine helikale Spur der Glasbeads aufgezeichnet, die si...

  11. The federations' agreement II on network utilisation; Die Verbaendevereinbarung II zur Netznutzung

    Energy Technology Data Exchange (ETDEWEB)

    Birkner, P. [Lech-Elektrizitaetswerke AG, Augsburg (Germany)

    2001-06-18

    Against the background of the experience of the regional supplier Lech Elektrizitaetswerke AG (LEW AG) and in the view of the operator of a regional distribution network the author deals with three complexes of themes: 1. Federations' Agreement II on network utilisation - Flexible instrument for competition or unsuited attempt? 2. What progress and obstacles are identifiable on route towards the implementation of the Federations' Agreement II on network utilisation? 3. What adjustment of existing sets of rules towards the requirements of competition is necessary? (orig.) [German] Vor dem Erfahrungshintergrund des Erfahrungsbereiches des Regionalversorgers Lech-Elektrizitaetswerke AG (LEW AG) und aus der Sicht des Betreibers eines regionalen Verteilungsnetzes behandelt der Verfasser die drei Themenkomplexe: 1. Verbaendevereinbarung II zur Netznutzung - Flexibles Instrument fuer den Wettbewerb oder ungeeigneter Versuch? 2. Welche Fortschritte und Hindernisse sind auf dem Wege der Umsetzung der Verbaendevereinbarung II zur Netznutzung erkennbar? 3. Welche Anpassung vorhandener Regelwerke an die Erfordernisse des Wettbewerbs sind notwendig? (orig.)

  12. Zur Entstehung, Konzeption und Wirkung des "Don Quijote" in der europäischen Literatur

    OpenAIRE

    Meyer-Minnemann, Klaus

    2007-01-01

    Wie kein zweiter Roman zieht der "Don Quijote" von Miguel de Cervantes seit nunmehr vier Jahrhunderten Generationen von Lesern immer wieder neu in seinen Bann. Abgefasst im Spanien des frühen 17. Jahrhunderts, das zu jener Zeit die Geschicke Europas wesentlich mitbestimmte, wurde der Roman bald zum Inbegriff der spanischen Literatur und Kultur. Von Madrid aus hat er auf den Rest Europas ausgestrahlt und Denker, Dichter, Künstler, Komponisten und später auch Filmemacher zur Auseinandersetzung ...

  13. Die Bildungskonzeption Wilhelm von Humboldts in der DDR. Ein Beitrag zur Rezeptions- und Wirkungsgeschichte des Neuhumanismus

    OpenAIRE

    Sroka, Wendelin

    1984-01-01

    Die Aneignung der Bildungskonzeption Humboldts in der DDR ist Gegenstand der vorliegenden Arbeit. Die Untersuchung enthält zwei Schwerpunkte: Zum einen wird das Humboldt-Bild der DDR dargestellt und interpretiert, zum anderen wird der Frage nachgegangen, inwieweit Prinzipien der Humboldtschen Bildungskonzeption in der Bildungstheorie der marxistisch-leninistischen Pädagogik zum Ausdruck kommen. Damit soll von erziehungswissenschaftlicher Seite ein Beitrag zur Tradierung des neuhumanistischen ...

  14. Der Einsatz von GVO: Empirische Ergebnisse zum Legitimierungsdruck und zur Corporate Social Responsibility im Agribusiness

    OpenAIRE

    Matthias Heyder; Ludwig Theuvsen

    2009-01-01

    Der Einsatz von Gentechnik zur Lebensmittelproduktion wird weltweit sehr unterschiedlich gehandhabt. Während in der neuen Welt großflächiger GVO-Anbau stattfindet, wird grüne Gentechnik in Europa aufgrund ihrer dort niedrigen gesellschaftlichen Legitimität in wesentlich geringerem Umfang eingesetzt. Hieraus ergeben sich für die Agribusiness-Unternehmen Probleme auf der Beschaffungsseite und aus Sicht der Corporate Social Responsibility (CSR). Durch Ergebnisse einer Online-Befragung von 170 Un...

  15. Evaluation und Erweiterung thermodynamischer Modelle zur Vorhersage von Wirkstofflöslichkeiten

    OpenAIRE

    Diedrichs, Anja

    2010-01-01

    Wirkstofflöslichkeiten in reinen Lösungsmitteln werden mit der Schroeder-van Laar-Gleichung berechnet. Zur Ermittlung der notwendigen Aktivitätskoeffizienten werden sieben verschiedene Modelle eingesetzt (ideale Mischung, Hansen-Löslichkeitsmodell, UNIFAC, Mod. UNIFAC (Do), COSMO-RS(Ol), NRTL-SAC, Pharma Mod. UNIFAC). Die Anzahl an berechenbaren Löslichkeitsdaten hängt aufgrund modellspezifischer Parameter vom Aktivitätskoeffizientenmodell ab. Der Vergleich mit verfügbaren experimentellen Dat...

  16. Neue Möglichkeiten zur Reduzierung von postoperativen Adhäsionen

    OpenAIRE

    Grund, Daniel

    2005-01-01

    Peritoneale Verwachsungen sind die häufigste Komplikation nach operativen Eingriffen. Sie haben abdominelle Beschwerden, chronische Ileuszustände und den akuten Ileus zur Folge. Diese peritonealen Reaktionen sind bei Neugeborenen und Säuglingen besonders ausgeprägt. Seit es operative abdominelle Eingriffe gibt, stellt das Vermeiden von peritonealen Adhäsionen ein zentrales Problem dar. Eine Vielzahl von Substanzen, lokal oder systemisch angewendet, wurde getestet, um Adhäsionen zu vermeiden...

  17. Erstellung einer Checkliste zur Beurteilung des Arbeitsplatzes eines Physiotherapeuten nach ergonomischen Gesichtspunkten

    OpenAIRE

    Gauermann, Christoph

    2013-01-01

    Einleitung: Arbeitsbedingte Erkrankungen des Muskel-Skelett-Systems sind unter Physiotherapeuten sehr häufig. Die präventive Forschung hat diese Berufsgruppe bisher kaum untersucht. In diesem Zusammenhang stellt die Veränderung des Arbeitsumfeldes einen vielversprechenden Ansatz für die Verhinderung arbeitsbedingter Erkrankungen dar. Das Ziel dieser Arbeit ist die Erstellung einer Checkliste zur Bewertung des Arbeitsplatzes eines Physiotherapeuten zu erstellen. Material und Methoden: ...

  18. Bildgebendes Multispektralsystem zur Serienfarbmessung an goniochromatischen Oberflächenbeschichtungen in der Automobil- und Zulieferindustrie

    OpenAIRE

    Jäger, Stefan

    2006-01-01

    In dieser Arbeit wird ein multispektrales bildgebendes System zur Farbmessung von Metall- und Perleffektlacken in einem industriellen Umfeld vorgestellt. Der Zweck des vorgeschlagenen Systems ist die Serienüberwachung und daraus folgend, die Steuerung von Lackierprozessen in der Automobil- und Zulieferindustrie, um das Qualitätsniveau anzuheben und Kosten zu reduzieren. Ausgehend von der Analyse der messtechnischen Anforderungen und den durch die Prozesstechnik gegebenen Randbedingungen wurde...

  19. Gefahrenfall und Entempirisierung. Anmerkungen zur Unterscheidung literarischer Akteure und Personen mit Bezug auf Oliver Jahraus’ Replik

    Directory of Open Access Journals (Sweden)

    David-Christopher Assmann

    2013-11-01

    Full Text Available Anmerkungen zur Unterscheidung literarischer Akteure und Personen mit Bezug auf Oliver Jahraus’ Replik. Die Replik setzt die seit der ersten Ausgabe von Textpraxis geführte Diskussion um eine Neufokussierung der systemtheoretischen Literaturwissenschaft fort. David-Christopher Assmann antwortet auf Oliver Jahraus’ Kritik an der Unterscheidung von Akteuren und Personen im Literatursystem, die jener in Ausgabe #3 in die Debatte eingeführt hat.

  20. Untersuchungen zur Spanbildung metallischer Werkstoffe anhand von in situ Röntgenbeugungsexperimenten

    OpenAIRE

    Brömmelhoff, Katrin Stefanie

    2016-01-01

    Zur Optimierung der Zerspanprozesse mit geometrisch bestimmter Schneide ist ein fundiertes Wissen über den Spanbildungsprozess notwendig, welches jedoch aufgrund der erschwerten messtechnischen Erfassbarkeit der Wirkzone stark limitiert ist. Moderne Quellen für hochenergetische Synchrotronröntgenstrahlung und neue Detektionsmöglichkeiten ermöglichen es nun, in situ Diffraktionsexperimente während des Zerspanprozesses in einem sehr kleinen Volumenelement durchzuführen. In der vorliegenden Arbe...

  1. Studien zur Zusammensetzung der Inhaltsstoffe getrockneter Heidelbeeren und Formulierungen zum Colon-Targeting von Anthocyanen

    OpenAIRE

    Oehme, Anett

    2011-01-01

    Die Ergebnisse verschiedener in vitro Untersuchungen und Tierstudien deuten darauf hin, dass Anthocyane zur Prävention und Therapie von intestinalen Erkrankungen wie akutem Durchfall oder chronisch entzündlichen Darm¬erkrankungen (CED) sowie Darmkrebs geeignete Naturstoffe sind. Mit bis zu 780 mg/100 g Frischgewicht weisen Heidelbeeren (Vaccinium myrtillus L.) besonders hohe Anthocyan¬¬gehalte auf. In getrockneter Form sind diese Beeren ein bewährtes Therapeutikum in der Volksheilkunde und we...

  2. Entwicklung und Anwendung von Methoden zur Bestimmung von Selen-Spezies in human-biologischem Material

    OpenAIRE

    Jäger, Thomas

    2014-01-01

    Selen ist essentieller Bestandteil von mehr als 25 physiologisch wichtigen menschlichen Enzymen. Der tägliche Bedarf wird überwiegend in Form von organischen Selenverbindungen wie Selenmethionin oder Selenocystein aus der Nahrung aufgenommen. Weitere Expositionswege sind die Einnahme von Nahrungsergänzungsmitteln zur Selensupplementation sowie die inhalative oder dermale Aufnahme von in erster Linie anorganischen Selenverbindungen oder elementarem Selen an Arbeitsplätzen der Selen-verarbeiten...

  3. Effiziente Verfahren zur Parallelisierung von EMV-Simulationen für den Entwurfsprozess integrierter Schaltungen

    Directory of Open Access Journals (Sweden)

    M. Barke

    2009-05-01

    Full Text Available In dieser Arbeit wird mit einer parallelisierten Version des PCG-Verfahrens (preconditioned conjugate gradient method eine effiziente Technik zur numerischen Lösung großer Gleichungssysteme vorgestellt. Der Anwendungsbereich ist dabei die Simulation numerischer Feldberechnungen (beispielsweise durch die Finite-Elemente-Methode. Es wird ferner auf eine Datenstruktur eingegangen, die für die spezielle Struktur der auftretenden Koeffizientenmatrizen geeignet ist.

  4. Effiziente Verfahren zur Parallelisierung von EMV-Simulationen für den Entwurfsprozess integrierter Schaltungen

    Science.gov (United States)

    Barke, M.; Preisner, T.; Mathis, W.

    2009-05-01

    In dieser Arbeit wird mit einer parallelisierten Version des PCG-Verfahrens (preconditioned conjugate gradient method) eine effiziente Technik zur numerischen Lösung großer Gleichungssysteme vorgestellt. Der Anwendungsbereich ist dabei die Simulation numerischer Feldberechnungen (beispielsweise durch die Finite-Elemente-Methode). Es wird ferner auf eine Datenstruktur eingegangen, die für die spezielle Struktur der auftretenden Koeffizientenmatrizen geeignet ist.

  5. Differentialindikation zur operativen Therapie der männlichen Inkontinenz nach radikaler Prostatektomie

    Directory of Open Access Journals (Sweden)

    Hübner W

    2005-01-01

    Full Text Available Inkontinenzraten nach radikaler Prostatektomie werden in 3–60 % angegeben [1–7], nach TUR/P etwa 1 % [8]. In den ersten 6 Monaten nach der Operation besteht die Möglichkeit einer Besserung der Symptomatik durch konservative Maßnahmen, nach mehr als 6 Monaten sind die Chancen, eine schwere Inkontinenz ohne operativen Eingriff zu heilen, nur noch gering. Ab diesem Zeitpunkt kann daher die Indikation zur operativen Sanierung einer Inkontinenz gestellt werden. Zur operativen Therapie der männlichen Inkontinenz stehen heute eine Reihe von Verfahren zur Verfügung, die sich in therapeutischem Potential, Komplexität, Preis, Limitierungen und Langzeiterfahrungen zwar unterscheiden, aber dennoch in vielen Fällen beim Versagen einer Methode auch alternativ eingesetzt werden können. Wir sind daher heute in der glücklichen Lage, in vielen Fällen für einzelne Patienten sogar mehrere Therapieoptionen anbieten zu können.

  6. Neuere Untersuchungen zur Prädiktion von EEG-Signalen bei Epilepsie

    Directory of Open Access Journals (Sweden)

    C. Niederhöfer

    2007-06-01

    Full Text Available Seit einigen Jahren ist die Analyse von EEG-Signalen bei Epilepsie Gegenstand zahlreicher wissenschaftlicher Arbeiten; Zielvorstellung ist dabei die Entwicklung von Verfahren zur Erkennung eines möglichen Voranfallszustandes. Im Vordergrund steht beispielsweise die Approximation einer so genannten effektiven Korrelationsdimension, die Bestimmung der maximalen Lyapunov-Exponenten, Detektionsverfahren für Muster bei Zellularen Nichtlinearen Netzwerken, die Bestimmung der mittleren Phasenkohärenz und Verfahren zur nichtlinearen Prädiktion von EEG-Signalen. Trotz umfangreicher Bemühungen kann bis heute eine Erkennung von Anfallsvorboten mit einer Sensitivität und Spezifität, die eine automatisierte Anfallsvorhersage ermöglichen würde, noch nicht durchgeführt werden. In diesem Beitrag werden neue Ergebnisse zur Prädiktion von EEG-Signalen bei Epilepsie vorgestellt. Dabei werden Signale, welche mittels intrakranieller electrocorticographischer (ECoG und stereoelectroencephalographischer (SEEG Ableitungen registriert wurden, segmentweise analysiert. Unter der Annahme, dass sich Änderungen des Systems ,,Gehirn" als Änderungen im Prädiktor, d.h. in seinen Systemparametern widerspiegeln, könnte eine nähere Betrachtung der Prädiktoreigenschaften zu einer Erkennung von Anfallsvorboten führen.

  7. Effect of hyperbaric oxygen therapy combined with autologous platelet concentrate applied in rabbit fibula fraction healing

    Directory of Open Access Journals (Sweden)

    Paulo Cesar Fagundes Neves

    2013-09-01

    Full Text Available OBJECTIVES: The purpose is to study the effects of hyperbaric oxygen therapy and autologous platelet concentrates in healing the fibula bone of rabbits after induced fractures. METHODS: A total of 128 male New Zealand albino rabbits, between 6-8 months old, were subjected to a total osteotomy of the proximal portion of the right fibula. After surgery, the animals were divided into four groups (n = 32 each: control group, in which animals were subjected to osteotomy; autologous platelet concentrate group, in which animals were subjected to osteotomy and autologous platelet concentrate applied at the fracture site; hyperbaric oxygen group, in which animals were subjected to osteotomy and 9 consecutive daily hyperbaric oxygen therapy sessions; and autologous platelet concentrate and hyperbaric oxygen group, in which animals were subjected to osteotomy, autologous platelet concentrate applied at the fracture site, and 9 consecutive daily hyperbaric oxygen therapy sessions. Each group was divided into 4 subgroups according to a pre-determined euthanasia time points: 2, 4, 6, and 8 weeks postoperative. After euthanasia at a specific time point, the fibula containing the osseous callus was prepared histologically and stained with hematoxylin and eosin or picrosirius red. RESULTS: Autologous platelet concentrates and hyperbaric oxygen therapy, applied together or separately, increased the rate of bone healing compared with the control group. CONCLUSION: Hyperbaric oxygen therapy and autologous platelet concentrate combined increased the rate of bone healing in this experimental model.

  8. Effect of hyperbaric oxygen therapy combined with autologous platelet concentrate applied in rabbit fibula fraction healing.

    Science.gov (United States)

    Neves, Paulo César Fagundes; Abib, Simone de Campos Vieira; Neves, Rogério Fagundes; Pircchio, Oronzo; Saad, Karen Ruggeri; Saad, Paulo Fernandes; Simões, Ricardo Santos; Moreira, Marcia Bento; Laurino, Cristiano Frota de Souza

    2013-09-01

    The purpose is to study the effects of hyperbaric oxygen therapy and autologous platelet concentrates in healing the fibula bone of rabbits after induced fractures. A total of 128 male New Zealand albino rabbits, between 6-8 months old, were subjected to a total osteotomy of the proximal portion of the right fibula. After surgery, the animals were divided into four groups (n = 32 each): control group, in which animals were subjected to osteotomy; autologous platelet concentrate group, in which animals were subjected to osteotomy and autologous platelet concentrate applied at the fracture site; hyperbaric oxygen group, in which animals were subjected to osteotomy and 9 consecutive daily hyperbaric oxygen therapy sessions; and autologous platelet concentrate and hyperbaric oxygen group, in which animals were subjected to osteotomy, autologous platelet concentrate applied at the fracture site, and 9 consecutive daily hyperbaric oxygen therapy sessions. Each group was divided into 4 subgroups according to a pre-determined euthanasia time points: 2, 4, 6, and 8 weeks postoperative. After euthanasia at a specific time point, the fibula containing the osseous callus was prepared histologically and stained with hematoxylin and eosin or picrosirius red. Autologous platelet concentrates and hyperbaric oxygen therapy, applied together or separately, increased the rate of bone healing compared with the control group. Hyperbaric oxygen therapy and autologous platelet concentrate combined increased the rate of bone healing in this experimental model.

  9. Autologous stem cell transplantation in the treatment of Hodgkin's disease

    Directory of Open Access Journals (Sweden)

    Tarabar Olivera

    2009-01-01

    Full Text Available Background/Aim. High-dose chemotherapy with autologous stem cell transplantacion (ASCT has shown to produce long-term disease-free survival in patients with chemotherapysensitive Hodgkin disease. The aim of the study was to evaluate efficacy of ASCT in the treatment of Hodgkin's disease. Methods. Between May 1997 and September 2008, 34 patients with Hodgkin's disease in median age of 25 (range 16-60 years, underwent ASCT. Autologous SCT were performed as consolidation therapy in one poor-risk patients with complete response (CR and in 10 patients in partial remission (PR after induction chemotherapy (32.5%, for chemosensitive relapse (CSR 1 and CSR 2 in 47% patients and in 20.5% patients with chemoresistant disease (CRD. All except one patient were in stage III/IV, extranodal site of disease had 24 patients and bulky disease had l0 patients. All the patients received a uniform preparatory regimen (BEAM. Results. An overall response was achieved in 30 of 32 evaluated patients, with 62.5% in CR and 31.25% in PR. After applying radiotherapy, two patients with PR after ASCT reached CR. Median follow-up was 15.5 months (range 3-133 months. The probability of overall survival (OS and progression-free survival (PFS at a 3-year period for all patients was 51.9 % and 48.9%, respectively. For 22 patients in CR after ASCT, a 3-year DFS was 66.5%. Estimates of 2.5-year survival were 14.3%, 61.9% and 100% for CRD, CSR and for patients with CR/PR, respectively (p < 0.01. However, when patients undergoing consolidation were analyzed separately from those in CSR, no significant difference in OS and PFS was observed according to the disease status at ASCT. In univariate analysis for OS, PFS i DFS, extranodal site of disease and disease bulk had no predictive value. Twelve patients died. The main cause of death was Hodgkin's disease. Transplant-related mortality was 3.1%. One patient with CRD developed secondary acute myeloid leukemia and died 28 months after the

  10. A prospective, randomized study of preoperative autologous donation for hip replacement surgery.

    Science.gov (United States)

    Billote, Dinna B; Glisson, Silas N; Green, David; Wixson, Richard L

    2002-08-01

    Preoperative autologous blood donation is commonly performed to meet potential perioperative transfusion needs and is a common practice prior to total hip arthroplasty. Using standardized transfusion guidelines, we prospectively analyzed the effectiveness of preoperative autologous donation as a method for decreasing allogeneic transfusion among patients undergoing unilateral primary total hip replacement who were eligible to donate autologous blood. Patients who were scheduled for primary total hip replacement surgery and who had a preoperative baseline hemoglobin level >or=120 g/L were randomized either to donate two units of blood (autologous donors) or not to donate any blood (nondonors). The donors and nondonors were compared with regard to demographic data, blood-loss volumes, hemoglobin measurements, and transfusion rates. Randomization continued until data were obtained from at least forty patients per treatment group. Of the ninety-six patients who completed the study, forty-two were autologous donors and fifty-four were nondonors. There were no significant differences between the donors and nondonors with regard to age, male:female ratio, estimated blood volume, baseline physical condition, or operative blood loss. The hemoglobin values at the time of enrollment (baseline), at the time of hospital discharge, and six weeks postoperatively were not significantly different between the two groups, although values at the time of admission (129 +/- 13 g/L versus 138 +/- 12 g/L) and in the recovery room (104 +/- 12 g/L versus 115 +/- 13 g/L) were significantly lower in the autologous donor group (p group required an allogeneic transfusion. Twenty-nine (69%) of the forty-two donors received an autologous transfusion. Thirty-four (41%) of eighty-two autologous units were wasted. At a charge of $379 per autologous unit, there was an additional cost of $758 for each patient in the donor group. Preoperative autologous donation provided no benefit for nonanemic

  11. Kardiogoniometrie: eine elektrokardiografische, nichtinvasive und belastungsfreie Methode zur Erkennung der kardialen Ischämie

    Directory of Open Access Journals (Sweden)

    Schüpbach, M.

    2009-08-01

    Full Text Available Einleitung: Eine dreidimensionale Erfassung der Erregungsabläufe am Herzen bringt zusätzlich Informationen zum EKG. Wegen prinzipieller Schwächen hat sich aber die traditionelle Vektorkardiographie nicht in der breiten klinischen Anwendung durchgesetzt. Wir haben eine neue, verbesserte vektorkardiographische Methode, die Kardiogoniometrie (KGM, entwickelt, die für die kardiologische Diagnostik eingesetzt werden kann. Methode: In der KGM werden die kardialen Potentiale in einem orthogonalen System gemessen und vektoriell summiert. Diese dreidimensionale Messung und Ortung des Herzpotentials ermöglichte die Schaffung von über 300 neuen Parametern, die u. a. die Erkennung der Myokardischaemie erlauben. Die Messung erfolgt mit vier Oberflächenelektroden in Ruhe ohne Belastung. Sie kommt in vier Formen zur Darstellung. 1. Die analogen orthogonalen Projektionen X, Y, Z zur Kontrolle der technischen Qualität der Messung, 2. Das Potential gemessen in den verschiedenen Zeitabschnitten des Herzzyklus, 3. Die Schlingen, 2D und 3D, zur Ortung des Potential im Herzen, 4. die Maximalvektoren der Vorhof- und Ventrikel-Depolarisation und der Ventrikel-Repolarisation. Resultate: Pilotstudien mit der Koronarographie als Referenzmethode zeigen im Vergleich zur Elektrokardiographie und der Ergometrie eine höhere Sensitivität und Spezifität, dies ohne Belastung des Patienten. In 696 Patienten (459 Männer hatte die KGM eine diagnostische Sensitivität von 84% und eine Spezifität von 90% verglichen mit dem Goldstandard der Koronarangiographie. Separate diagnostische Scores zur Erkennung der koronaren Herzkrankheit bei Bestehen einer Low voltage, eines Linksschenkelblocks, eines Rechtsschenkelblocks oder Vorhofflimmern wurden retrospektiv bestimmt. Diskussion: Die Kardiogoniometrie (KGM ist imstande auf technisch einfache, trotzdem physikalisch korrekte und zuverlässige Weise die Herzpotentiale dreidimensional zu erfassen. Dadurch konnte eine Verbesserung

  12. Mobilization of hematopoietic progenitor cells for autologous transportation: consensus recommendations

    Directory of Open Access Journals (Sweden)

    Fernando Barroso Duarte

    Full Text Available SUMMARY Selected patients with certain hematological malignancies and solid tumors have the potential to achieve long-term survival with autologous hematopoietic progenitor cell transplant. The collection of these cells in peripheral blood avoids multiple bone marrow aspirations, results in faster engraftment and allows treatment of patients with infection, fibrosis, or bone marrow hypocellularity. However, for the procedure to be successful, it is essential to mobilize a sufficient number of progenitor cells from the bone marrow into the blood circulation. Therefore, a group of Brazilian experts met in order to develop recommendations for mobilization strategies adapted to the reality of the Brazilian national health system, which could help minimize the risk of failure, reduce toxicity and improve the allocation of financial resources.

  13. Surgical management and autologous intestinal reconstruction in short bowel syndrome.

    Science.gov (United States)

    Hommel, Matthijs J; van Baren, Robertine; Haveman, Jan Willem

    2016-04-01

    Short bowel syndrome (SBS) is a serious condition with considerable morbidity and mortality. When treatment with parenteral nutrition fails and life-threatening complications occur, autologous intestinal reconstruction (AIR) should be considered before intestinal transplantation (ITx). Single or combined ITx should be reserved for patients with severe liver disease and as last resort in the treatment of SBS. Longitudinal intestinal lengthening and tailoring (LILT) has proven its value in AIR, but its availability depends on the expertise of the surgeons. Serial transverse enteroplasty (STEP) has similar success rates as LILT and fewer patients progress to ITx. STEP is also applicable at small bowel dilatation in ultra-short bowel syndrome. The scope may be widened when duodenal dilatation can be treated as well. Spiral intestinal lengthening and tailoring (SILT) is a promising alternative. More research is needed to confirm these findings. Therefore we suggest an international data registry for all intestinal lengthening procedures. Copyright © 2016 Elsevier Ltd. All rights reserved.

  14. Transcriptomic biomarkers of altered erythropoiesis to detect autologous blood transfusion.

    Science.gov (United States)

    Salamin, Olivier; Mignot, Jonathan; Kuuranne, Tiia; Saugy, Martial; Leuenberger, Nicolas

    2018-03-01

    Autologous blood transfusion is a powerful means of improving performance and remains one of the most challenging methods to detect. Recent investigations have identified 3 candidate reticulocytes genes whose expression was significantly influenced by blood transfusion. Using quantitative reverse transcription polymerase chain reaction as an alternative quantitative method, the present study supports that delta-aminolevulinate synthase 2 (ALAS2), carbonic anhydrase (CA1), and solute carrier family 4 member 1 (SLC4A1) genes are down-regulated post-transfusion. The expression of these genes exhibited stronger correlation with immature reticulocyte fraction than with reticulocytes percentage. Moreover, the repression of reticulocytes' gene expression was more pronounced than the diminution of immature reticulocyte fraction and reticulocyte percentage following blood transfusion. It suggests that the 3 candidate genes are reliable predictors of bone marrow's response to blood transfusion and that they represent potential biomarkers for the detection of this method prohibited in sports. Copyright © 2017 John Wiley & Sons, Ltd.

  15. Immunisation of colorectal cancer patients with autologous tumour cells

    DEFF Research Database (Denmark)

    Diederichsen, Axel Cosmus Pyndt; Stenholm, A C; Kronborg, O

    1998-01-01

    . There was an inverse relation between survival and HLA class II expression. This highlights an essential problem, in the absence of CD80 expression the expression of HLA class II may induce anergy. In future attempts to develop improved vaccines this problem should be addressed.......Patients with colorectal cancer were entered into a clinical phase I trial of immunotherapy with an autologous tumour cell/bacillus Calmette-Guerin (BCG) vaccine. We attempted to describe the possible effects and side effects of the immunisation, and further to investigate whether expression...... of immune-response-related surface molecules on the tumour cells in the vaccine correlated with survival. The first and second vaccine comprised of 107 irradiated tumour cells mixed with BCG, the third of irradiated tumour cells only. Thirty-nine patients were considered, but only 6 patients fulfilled...

  16. MR imaging of autologous chondrocyte implantation of the knee

    Energy Technology Data Exchange (ETDEWEB)

    James, S.L.J.; Connell, D.A.; Saifuddin, A.; Skinner, J.A.; Briggs, T.W.R. [RNOH Stanmore, Department of Radiology, Stanmore, Middlesex (United Kingdom)

    2006-05-15

    Autologous chondrocyte implantation (ACI) is a surgical technique that is increasingly being used in the treatment of full-thickness defects of articular cartilage in the knee. It involves the arthroscopic harvesting and in vitro culture of chondrocytes that are subsequently implanted into a previously identified chondral defect. The aim is to produce a repair tissue that closely resembles hyaline articular cartilage that gradually becomes incorporated, restoring joint congruity. Over the long term, it is hoped that this will prevent the progression of full-thickness articular cartilage defects to osteoarthritis. This article reviews the indications and operative procedure performed in ACI. Magnetic resonance imaging (MRI) sequences that provide optimal visualization of articular cartilage in the post-operative period are discussed. Normal appearances of ACI on MRI are presented along with common complications that are encountered with this technique. (orig.)

  17. Radiation-induced autologous in situ tumor vaccines

    International Nuclear Information System (INIS)

    Guha, Chandan

    2014-01-01

    Radiation therapy (RT) has been used as a definitive treatment for many solid tumors. While tumoricidal properties of RT are instrumental for standard clinical application, irradiated tumors can potentially serve as a source of tumor antigens in vivo, where dying tumor cells would release tumor antigens and danger signals and serve as autologous in situ tumor vaccines. Using murine tumor models of prostate, metastatic lung cancer and melanoma, we have demonstrated evidence of radiation-enhanced tumor-specific immune response that resulted in improved primary tumor control and reduction in systemic metastasis and cure. We will discuss the immunogenic properties of RT and determine how immunotherapeutic approaches can synergize with RT in boosting immune cells cell function. (author)

  18. Randomized phase II trial of autologous dendritic cell vaccines versus autologous tumor cell vaccines in metastatic melanoma: 5-year follow up and additional analyses.

    Science.gov (United States)

    Dillman, Robert O; Cornforth, Andrew N; Nistor, Gabriel I; McClay, Edward F; Amatruda, Thomas T; Depriest, Carol

    2018-03-06

    Despite improved survival following checkpoint inhibitors, there is still a potential role for anti-cancer therapeutic vaccines. Because of biological heterogeneity and neoantigens resulting from each patient's mutanome, autologous tumor may be the best source of tumor-associated antigens (TAA) for vaccines. Ex vivo loading of autologous dendritic cells with TAA may be associated with superior clinical outcome compared to injecting irradiated autologous tumor cells. We conducted a randomized phase II trial to compare autologous tumor cell vaccines (TCV) and autologous dendritic cell vaccines (DCV) loaded with autologous TAA. Short-term autologous tumor cell lines were established from metastatic tumor. Vaccines were admixed with 500 micrograms of GM-CSF and injected weekly for 3 weeks, then at weeks 8, 12,16, 20, and 24. The primary endpoint was overall survival. Secondary objectives were identification of adverse events, and results of delayed type hypersensitivity (DTH) reactions to intradermal tumor cell injections. Forty-two patients were randomized. All were followed from randomization until death or for five years; none were lost to follow-up. DCV was associated with longer survival: median 43.4 versus 20.5 months (95% CI, 18.6 to > 60 versus 9.3 to 32.3 months) and a 70% reduction in the risk of death (hazard ratio = 0.304, p = 0.0053, 95% CI, 0.131 to 0.702). Tumor DTH reactions were neither prognostic nor predictive. The most common treatment-related adverse events were mild to moderate local injection site reactions and flu-like symptoms; but grade 2 treatment-related adverse events were more frequent with TCV. Serum marker analyses at week-0 and week-4 showed that serum markers were similar at baseline in each arm, but differed after vaccination. This is the only human clinical trial comparing DCV and TCV as platforms for autologous TAA presentation. DCV was associated with minimal toxicity and long-term survival in patients with metastatic

  19. Human induced pluripotent stem cells on autologous feeders.

    Directory of Open Access Journals (Sweden)

    Kazutoshi Takahashi

    Full Text Available BACKGROUND: For therapeutic usage of induced Pluripotent Stem (iPS cells, to accomplish xeno-free culture is critical. Previous reports have shown that human embryonic stem (ES cells can be maintained in feeder-free condition. However, absence of feeder cells can be a hostile environment for pluripotent cells and often results in karyotype abnormalities. Instead of animal feeders, human fibroblasts can be used as feeder cells of human ES cells. However, one still has to be concerned about the existence of unidentified pathogens, such as viruses and prions in these non-autologous feeders. METHODOLOGY/PRINCIPAL FINDINGS: This report demonstrates that human induced Pluripotent Stem (iPS cells can be established and maintained on isogenic parental feeder cells. We tested four independent human skin fibroblasts for the potential to maintain self-renewal of iPS cells. All the fibroblasts tested, as well as their conditioned medium, were capable of maintaining the undifferentiated state and normal karyotypes of iPS cells. Furthermore, human iPS cells can be generated on isogenic parental fibroblasts as feeders. These iPS cells carried on proliferation over 19 passages with undifferentiated morphologies. They expressed undifferentiated pluripotent cell markers, and could differentiate into all three germ layers via embryoid body and teratoma formation. CONCLUSIONS/SIGNIFICANCE: These results suggest that autologous fibroblasts can be not only a source for iPS cells but also be feeder layers. Our results provide a possibility to solve the dilemma by using isogenic fibroblasts as feeder layers of iPS cells. This is an important step toward the establishment of clinical grade iPS cells.

  20. Use of autologous platelet - Rich plasma in the treatment of intrabony defects

    Directory of Open Access Journals (Sweden)

    Sharath K Shetty

    2009-01-01

    Treatment of intrabony defects by autologous PRP gel alone caused significant soft tissue clinical improvement as well as hard tissue defect fill as evidenced by SSD view in spiral computed tomography.

  1. Local administration of autologous platelet-rich plasma in a female patient with skin ulcer defect

    Directory of Open Access Journals (Sweden)

    S M Noskov

    2011-01-01

    Full Text Available The paper describes a clinical observation of the efficiency of local therapy with autologous platelet-rich plasma for .skin ulcer defect in a female with chronic lymphocytic leukemia

  2. Rapid deterioration of preexisting renal insufficiency after autologous mesenchymal stem cell therapy

    Directory of Open Access Journals (Sweden)

    Jun-Seop Kim

    2017-06-01

    Full Text Available Administration of autologous mesenchymal stem cells (MSCs has been shown to improve renal function and histological findings in acute kidney injury (AKI models. However, its effects in chronic kidney disease (CKD are unclear, particularly in the clinical setting. Here, we report our experience with a CKD patient who was treated by intravenous infusion of autologous MSCs derived from adipose tissue in an unknown clinic outside of Korea. The renal function of the patient had been stable for several years before MSC administration. One week after the autologous MSC infusion, the preexisting renal insufficiency was rapidly aggravated without any other evidence of AKI. Hemodialysis was started 3 months after MSC administration. Renal biopsy findings at dialysis showed severe interstitial fibrosis and inflammatory cell infiltration, with a few cells expressing CD34 and CD117, 2 surface markers of stem cells. This case highlights the potential nephrotoxicity of autologous MSC therapy in CKD patients.

  3. Comparison of immune reconstitution after allogeneic vs. autologous stem cell transplantation in 182 pediatric recipients

    Directory of Open Access Journals (Sweden)

    V. Wiegering

    2017-03-01

    Conclusion: Children undergoing a HSCT show a different pattern of immune reconstitution in the allogeneic and autologous setting. This might influence the outcome and should affect the clinical handling of infectious prophylaxis and re-vaccinations.

  4. Ultrasound Diagnosis and Treatment of Breast Lumps after Breast Augmentation with Autologous Fat Grafting

    Directory of Open Access Journals (Sweden)

    Masaaki Shida, MD, PhD

    2017-12-01

    Conclusions:. The appropriate treatment for breast lumps after breast augmentation with autologous fat grafting must be selected according to the nature of the lumps. Ultrasound is essential for diagnosing the breast lump type and determining the best treatment.

  5. Successful closure of persistent oro-cutaneous fistulas by injection of autologous adipose-derived stem cells: a case report [Erfolgreiche Behandlung persistierender oro-kutaner Fisteln durch Injektion mit autologen Fettstammzellen: ein Fallbericht

    Directory of Open Access Journals (Sweden)

    Föll, D. A.

    2013-07-01

    Full Text Available [english] Introduction: Oro-cutaneous fistulas are a possible complication of oro-facial surgery. If recurrent they are difficult to treat and greatly affect the patient's functional and aesthetic integrity besides carrying the risk of infection. Usage of concentrated adipose-derived stem cells for chronic wounds is a new and experimental treatment strategy, so far only applied in a few selected studies. We report the case of two oro-cutaneous fistulas in one patient treated with adipose-derived stem cells leading to closure of the fistulas.Case description: The patient was a 57-year old female immunosuppressed liver and kidney recipient who presented with a large mandibular and soft tissue defect after resection and irradiation of a squamous-cell carcinoma of the tongue base. After radical debridement, hardware removal and soft tissue reconstruction using a second free flap two oro-cutaneous fistulas reoccurred. Conventional surgical methods failed to consolidate the fistulas due to the severe radiation of the oral mucosa. Therefore autologous adipose-derived stem cells were extracted from lipoaspirate and applied in the surrounding fistula tissue in one session using the Cytori Celution system. The fistulas closed spontaneously during the postoperative course within 4 weeks and remain closed after 6 months.Conclusion: To our knowledge this is the first report of successful application of adipose-derived stem cells to two oro-cutaneous fistulas. Innovative stem cell therapy in combination with well established surgical methods can be a useful treatment strategy in certain cases.[german] Einleitung: Oro-kutane Fisteln sind eine mögliche Komplikation eines oro-fazialen Eingriffs. Bei Therapieresistenz können solche Fisteln zu hohem Leidensdruck führen und stellen eine ständige Infektionsgefahr dar. Die Applikation von konzentrierten Stammzellen aus Fettgewebe zur Behandlung chronischer Wunden ist eine neue und experimentelle Therapiestrategie

  6. Medienkompetenz und E-Portfolios für die Sekundarstufe I – ein Weg zur Medienbildung

    Directory of Open Access Journals (Sweden)

    Bernhard Ertl

    2015-06-01

    Full Text Available Medienkompetenz und digital literacy gehören inzwischen zu den Schlüsselkompetenzen und sind ein Teil der Medienbildung von Kindern und Jugendlichen. Medienbildung und/oder die dazu gehörigen Kompetenzen werden jedoch in Lehrplänen oft unterschiedlich verortet und definiert. Hierbei stellt sich die Frage, inwieweit kompetenzorientiertes Lernen den Ansprüchen an Medienbildung gerecht wird bzw. zur Medienbildung beitragen kann. Das Projekt EUfolio implementiert E-Portfolios in verschiedenen europäischen Klassen der Sekundarstufe I und realisiert dabei einen integrativen, fächerübergreifenden Ansatz. Dieser zielt primär auf den Erwerb von Schlüsselqualifikationen, kann jedoch implizit, etwa im Rahmen des reflektierenden Lernens mit E-Portfolios, zur Medienbildung beitragen. Der Artikel charakterisiert die Konzepte der Medienkompetenz und Medienbildung und diskutiert auf Basis des Projekts Eufolio, wie kompetenzorientiertes Lernen mit E-Portfolios einen Beitrag zur Medienbildung leisten kann.

  7. Renewable energies in Germany. Data on the development in 2016; Erneuerbare Energien in Deutschland. Daten zur Entwicklung im Jahr 2016

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2017-03-15

    The Working Group on Renewable Energy Statistics (AGEE-Stat), which regularly evaluates the use of renewable energies on behalf of the German Federal Ministry of Economics and Energy, has made an initial assessment of the development of renewable energies in Germany on the basis of available, mostly provisional data of 2016. The present background paper describes the previous findings for the areas of electricity, heat and transport, supplemented by figures on economic significance as well as on emission reductions through renewable energies. [German] Die Arbeitsgruppe Erneuerbare Energien-Statistik (AGEE-Stat), die im Auftrag des Bundesministeriums fuer Wirtschaft und Energie regelmaessig die Nutzung der erneuerbaren Energien bilanziert, hat auf der Grundlage verfuegbarer, zumeist vorlaeufiger Daten eine erste Abschaetzung zur Entwicklung der erneuerbaren Energien in Deutschland im Jahr 2016 erstellt. Das vorliegende Hintergrundpapier beschreibt die bisherigen Erkenntnisse fuer die Bereiche Strom, Waerme und Verkehr, ergaenzt um Zahlen zur wirtschaftlichen Bedeutung sowie zur Emissionsvermeidung durch erneuerbare Energien.

  8. The composite of bone marrow concentrate and PRP as an alternative to autologous bone grafting.

    Directory of Open Access Journals (Sweden)

    Mohssen Hakimi

    Full Text Available One possible alternative to the application of autologous bone grafts represents the use of autologous bone marrow concentrate (BMC. The purpose of our study was to evaluate the potency of autologous platelet-rich plasma (PRP in combination with BMC. In 32 mini-pigs a metaphyseal critical-size defect was surgically created at the proximal tibia. The animals were allocated to four treatment groups of eight animals each (1. BMC+CPG group, 2. BMC+CPG+PRP group, 3. autograft group, 4. CPG group. In the BMC+CPG group the defect was filled with autologous BMC in combination with calcium phosphate granules (CPG, whereas in the BMC+CPG+PRP group the defect was filled with the composite of autologous BMC, CPG and autologous PRP. In the autograft group the defect was filled with autologous cancellous graft, whereas in the CPG group the defect was filled with CPG solely. After 6 weeks radiological and histomorphometrical analysis showed significantly more new bone formation in the BMC+CPG+PRP group compared to the BMC+CPG group and the CPG group. There were no significant differences between the BMC+CPG+PRP group and the autograft group. In the PRP platelets were enriched significantly about 4.7-fold compared to native blood. In BMC the count of mononuclear cells increased significantly (3.5-fold compared to the bone marrow aspirate. This study demonstrates that the composite of BMC+CPG+PRP leads to a significantly higher bone regeneration of critical-size defects at the proximal tibia in mini-pigs than the use of BMC+CPG without PRP. Furthermore, within the limits of the present study the composite BMC+CPG+PRP represents a comparable alternative to autologous bone grafting.

  9. The Efficacy and Safety of Autologous Transfusion in Unilateral Total Knee Arthroplasty

    OpenAIRE

    Yoo, Moon-Jib; Park, Hee-Gon; Ryu, Jee-Won; Kim, Jeong-Sang

    2015-01-01

    Purpose Although allogeneic blood transfusion is the most common method of transfusion in total knee arthroplasty (TKA), there are reports showing significant decrease in the amount of allogeneic transfusion and incidence of side effects after combined use of autologous transfusion. The purpose of this study is to investigate the efficacy of using an autologous transfusion device in TKA. Materials and Methods Patients who underwent TKA at our institution from January 2003 to January 2014 were...

  10. Comparative analysis of autologous blood transfusion and allogeneic blood transfusion in surgical patients

    OpenAIRE

    Long, Miao-Yun; Liu, Zhong-Han; Zhu, Jian-Guang

    2014-01-01

    Objective: To investigate application effects of autologous blood transfusion and allogeneic blood transfusion in surgically treated patients receiving spine surgery, abdomen surgery and ectopic pregnancy surgery. Methods: 130 patients who would undergo selective operations were divided into autologous transfusion group and allogeneic transfusion group. Both groups received the same anesthesia, and there was no significant difference in transfusion volume or fluid infusion volume. Results: Th...

  11. The composite of bone marrow concentrate and PRP as an alternative to autologous bone grafting.

    Science.gov (United States)

    Hakimi, Mohssen; Grassmann, Jan-Peter; Betsch, Marcel; Schneppendahl, Johannes; Gehrmann, Sebastian; Hakimi, Ahmad-Reza; Kröpil, Patric; Sager, Martin; Herten, Monika; Wild, Michael; Windolf, Joachim; Jungbluth, Pascal

    2014-01-01

    One possible alternative to the application of autologous bone grafts represents the use of autologous bone marrow concentrate (BMC). The purpose of our study was to evaluate the potency of autologous platelet-rich plasma (PRP) in combination with BMC. In 32 mini-pigs a metaphyseal critical-size defect was surgically created at the proximal tibia. The animals were allocated to four treatment groups of eight animals each (1. BMC+CPG group, 2. BMC+CPG+PRP group, 3. autograft group, 4. CPG group). In the BMC+CPG group the defect was filled with autologous BMC in combination with calcium phosphate granules (CPG), whereas in the BMC+CPG+PRP group the defect was filled with the composite of autologous BMC, CPG and autologous PRP. In the autograft group the defect was filled with autologous cancellous graft, whereas in the CPG group the defect was filled with CPG solely. After 6 weeks radiological and histomorphometrical analysis showed significantly more new bone formation in the BMC+CPG+PRP group compared to the BMC+CPG group and the CPG group. There were no significant differences between the BMC+CPG+PRP group and the autograft group. In the PRP platelets were enriched significantly about 4.7-fold compared to native blood. In BMC the count of mononuclear cells increased significantly (3.5-fold) compared to the bone marrow aspirate. This study demonstrates that the composite of BMC+CPG+PRP leads to a significantly higher bone regeneration of critical-size defects at the proximal tibia in mini-pigs than the use of BMC+CPG without PRP. Furthermore, within the limits of the present study the composite BMC+CPG+PRP represents a comparable alternative to autologous bone grafting.

  12. Latissimus Dorsi and Immediate Fat Transfer (LIFT for Complete Autologous Breast Reconstruction

    Directory of Open Access Journals (Sweden)

    James M. Economides, MD

    2018-01-01

    Conclusion:. Autologous augmentation of the LD flap with lipotransfer has been used to avoid placement of an implant. We improve the technique by performing lipotransfer during index reconstruction. Furthermore, we perform lipotransfer prior to disorigination of the LD muscle to minimize trauma to the flap and increase the efficiency of fat grafting. Our experience demonstrates that this technique is a viable autologous alternative to microsurgical breast reconstruction.

  13. Persistent seropositivity for yellow fever in a previously vaccinated autologous hematopoietic stem cell transplantation recipient.

    Science.gov (United States)

    Hayakawa, Kayoko; Takasaki, Tomohiko; Tsunemine, Hiroko; Kanagawa, Shuzo; Kutsuna, Satoshi; Takeshita, Nozomi; Mawatari, Momoko; Fujiya, Yoshihiro; Yamamoto, Kei; Ohmagari, Norio; Kato, Yasuyuki

    2015-08-01

    The duration of a protective level of yellow fever antibodies after autologous hematopoietic stem cell transplantation in a previously vaccinated person is unclear. The case of a patient who had previously been vaccinated for yellow fever and who remained seropositive for 22 months after autologous peripheral blood stem cell transplantation for malignant lymphoma is described herein. Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

  14. Treatment of osteonecrosis of the femoral head using autologous cultured osteoblasts: a case report

    Directory of Open Access Journals (Sweden)

    Kim Seok-Jung

    2008-02-01

    Full Text Available Abstract Introduction Osteonecrosis of the femoral head is a progressive disease that leads to femoral head collapse and osteoarthritis. Our goal in treating osteonecrosis is to preserve, not to replace, the femoral head. Case presentation We present the case of a patient with bilateral osteonecrosis of the femoral head treated with autologous cultured osteoblast injection. Conclusion Although our experience is limited to one patient, autologous cultured osteoblast transplantation appears to be effective for treating the osteonecrosis of femoral head.

  15. Autologous bone graft versus demineralized bone matrix in internal fixation of ununited long bones

    OpenAIRE

    Rubenbauer Bianka; Löffler Thomas; Zaspel Johannes; Wittmann Alexandra; Pieske Oliver; Trentzsch Heiko; Piltz Stefan

    2009-01-01

    Abstract Background Non-unions are severe complications in orthopaedic trauma care and occur in 10% of all fractures. The golden standard for the treatment of ununited fractures includes open reduction and internal fixation (ORIF) as well as augmentation with autologous-bone-grafting. However, there is morbidity associated with the bone-graft donor site and some patients offer limited quantity or quality of autologous-bone graft material. Since allogene bone-grafts are introduced on the marke...

  16. Current developments in the tissue engineering of autologous heart valves: moving towards clinical use.

    Science.gov (United States)

    Apte, Sameer S; Paul, Arghya; Prakash, Satya; Shum-Tim, Dominique

    2011-01-01

    The use of tissue-engineering methods to create autologous heart valve constructs has the potential to overcome the fundamental drawbacks of more traditional valve prostheses. Traditional mechanical valves, while durable, increase the risk for endocarditis and thrombogenesis, and require the recipient to continue lifelong anticoagulant therapy. Homograft or xenograft heart valve prostheses are associated with immune reaction and progressive deterioration with limited durability. Most importantly, neither option is capable of growth and remodeling in vivo and both options place the patient at risk for valve-related complications and reoperation. These shortcomings have prompted the application of tissue-engineering techniques to create fully autologous heart valve replacements. Future clinically efficacious tissue-engineered autologous valves should be nonthrombogenic, biocompatible, capable of growth and remodeling in vivo, implantable with current surgical techniques, hemodynamically perfect, durable for the patient's life and most importantly, significantly improve quality of life for the patient. In order to meet these expectations, the nature of the ideal biochemical milieu for conditioning an autologous heart valve will need to be elucidated. In addition, standardized criteria by which to quantitatively evaluate a tissue-engineered heart valve, as well as noninvasive analytical techniques for use in long-term animal models, will be required. This article highlights the advances, challenges and future clinical prospects in the field of tissue engineering of autologous heart valves, focusing on progress made by studies that have investigated a fully autologous, tissue-engineered pulmonary valve replacement in vivo.

  17. Easy-to-prepare autologous platelet-rich plasma in the treatment of refractory corneal ulcers.

    Science.gov (United States)

    Wu, Tzu En; Chen, Chiung Ju; Hu, Chao-Chien; Cheng, Cheng-Kuo

    2015-01-01

    As platelets are rich in growth factors for tissue regeneration, autologous platelet-rich plasma (PRP) has been used to treat some refractory corneal defects. Although PRP is effective, the cost of its preparation is very high. This article presents three cases of refractory corneal ulcer under the prescription of autologous PRP. The autologous PRP used in these cases was easily prepared in the blood bank laboratory. In this paper, we collected three patients with refractory corneal ulcer who were unresponsive to conventional treatment. The patients presented with neurotrophic ulcer, exposure corneal ulcer, and limbal deciency with corneal ulcer after hepatitic keratitis. Although we easily prepared autologous PRP eye drops using simple laboratory centrifugation, this preparation still had a clinical effect on corneal defect. The mean intervention time was 24 ± 6.9 days. The case with exposure corneal ulcer had significant wound healing and the other two cases felt subjective symptom relief. There were some clinical improvements of refractory corneal ulcers in our three cases. We present the clinical results of three cases and report an easy procedure for the preparation of autologous PRP. Autologous PRP prepared simply in the laboratory, it may be an alternative option for treating refractory corneal ulcer.

  18. Sind Jugendliche "online" besser zu erreichen? Zur Internetnutzung bei Stresspräventionsmaßnahmen für Jugendliche

    OpenAIRE

    Fridrici, Mirko; Beyer, Anke; Lohaus, Arnold

    2008-01-01

    Jugendliche gelten als eine schwierige Zielgruppe für Gesundheitsförderungsangebote. Vor diesem Hintergrund wird der Frage nachgegangen, welche Möglichkeiten und Grenzen sich bei der Nutzung des Internets zur Verbreitung von Angeboten zur Stressprävention ergeben. Dazu werden die Ergebnisse zweier Studien berichtet. In der ersten Studie mit 1.988 Jugendlichen der siebten bis neunten Klasse wurde untersucht, in welchem Ausmaß eine Internetnutzung bei den Schülern verbreitet ist und welchen Ste...

  19. Autologous tumor vaccine lowering postsurgical recurrent rate of hepatocellular carcinoma.

    Science.gov (United States)

    Peng, Baogang; Liang, Lijian; Chen, Zubing; He, Qiang; Kuang, Ming; Zhou, Fan; Lu, Mingde; Huang, Jiefu

    2006-01-01

    A tumor vaccine consisting of formalin-fixed hepatocellular carcinoma (HCC) tissue fragments, biodegradable sustained-releasers of granulocyte-macrophage-colony stimulating factor (GM-CSF) and interleukin-2 (IL-2), and an adjuvant was developed. The aim of this study was to evaluate the effects of autologous tumor vaccine for protective immunity against HCC. C57BL/6J mice were immunized intradermally with the Hepa1-6 tumor vaccine on day 0 and 7, followed by intrahepatic challenge with live Hepa1-6 cells. On day 21, the tumor volumes were measured and the effect of tumor vaccine was evaluated. Lymphocytes from the immunized mice were cultured and the specific cytotoxicity against Hepa1-6 was accessed. Then from March 1999 to June 2003, 67 patients with HCC undergoing curative resection were randomly divided into a tumor vaccine group (n = 32) and a control group (n = 35). Patients in the tumor vaccine group received 3 vaccinations at a 2-week interval and the control group only adjuvant treatment for symptoms. A delayed-type-hypersensitivity test was performed before and after vaccination. Primary endpoint was time to first recurrence and recurrent rates were analyzed. The tumor vaccine protected 87% of syngeneic mice from Hepa1-6 cells inoculation. In an in vitro experiment, splenocytes from the vaccinated mice exhibited a 56% lytic activity against the Hepa1-6 cells at an effector/target (E/T) ratio of 5, whereas they did not exhibit such activity against other tumor cells. The cytotoxic activity was inhibited by the treatment with anti-CD3, anti-CD8, and anti-MHC-class II monoclonal antibodies but not with anti-CD4 and anti-MHC-class I antibodies. In clinical trial, thirty-two patients had completed the tumor vaccine procedure and no essential adverse effect occurred. The follow-up averaged 33.6 months (range from 15 to 54 months). The recurrent rate was significantly better in the tumor vaccine group (1 year, 12.6%; 2 years, 35.9%; 3 years, 54%) than in the

  20. Egypt`s potential for geothermal energy use and underground storage of thermal energy; Moeglichkeiten zur Nutzung geothermischer Energie und zur unterirdischen thermischen Energiespeicherung in Aegypten

    Energy Technology Data Exchange (ETDEWEB)

    Abbas, A.M.; Sanner, B.; Knoblich, K. [Giessen Univ. (Germany). Inst. fuer Angewandte Geowissenschaften

    1997-12-01

    Egypt belongs to the arid to extremely arid zone. Hot springs or wells are chiefly distributed over the areas of the Golf of Suez shoreline, along the Red Sea coast and in the Bahariya, Dakhla and Kharga oasis in the Western Desert. The Red Sea with it`s branches into the Gulf of Suez and the Gulf of Aqaba/Jordan valley is the northern end of the East African Rift, which is tectonically active and yields further south known geothermal resources (e.g. in Kenya). Thus, a relatively high het flow zone exists on the eastern border of the Gulf of Suez, on Sinai peninsula. The hot springs of Ayun Musa, Hammam Faraon and El Sokhna are located there. Hammam Faroun is the hottest spring in Egypt with water temperature of approx. 70 C. This paper compiles previous studies from Egypt to elucidate the geothermal potential of Egypt and opportunities to make use of it. (orig.) [Deutsch] Aegypten gehoert zur ariden bis extrem ariden Zone. Heisse Quellen oder erbohrte heisse Waesser finden sich hauptsaechlich entlang der Kueste des Golfs von Suez und des Roten Meeres, sowie in den Oasen Bahariya, Dakhla und Kharga in der westlichen Wueste. Das Rote Meer mit seinen Verzweigungen in den Golf von Suez und in den Golf von Akaba/Jordangraben bildet den noerdlichen Abschluss des Ostafrikanischen Grabensystems, das tektonisch sehr aktiv ist und weiter suedlich (z.B. in Kenia) bekannte geothermische Ressourcen bietet. Eine Zone mit hohem geothermischen Waermefluss befindet sich dementsprechend auch am oestlichen Rand des Golfs von Suez, der zur Halbinsel Sinai gehoehrt. Hier werden die heissen Quellen von Ayun Musa, Hammam Faraon und El Sokhna angetroffen. Hammam Faraon ist die heisseste Quelle Aegyptens mit Wassertemperaturen von etwa 70 C. Die vorliegende Arbeit versucht, einige fruehere Studien aus Aegypten zusammenzufassen und das geothermische Potential Aegyptens mit den Moeglichkeiten seiner Nutzung aufzuzeigen. (orig.)

  1. Indications for surgical resection of benign pancreatic tumors; Indikationen zur chirurgischen Therapie benigner Pankreastumoren

    Energy Technology Data Exchange (ETDEWEB)

    Isenmann, R.; Henne-Bruns, D. [Chirurgische Universitaetsklinik, Klinik fuer Allgemein-, Viszeral- und Transplantationschirurgie, Ulm (Germany)

    2008-08-15

    Benign pancreatic tumors should undergo surgical resection when they are symptomatic or - in the case of incidental discovery - bear malignant potential. This is the case for the majority of benign pancreatic tumors, especially for intraductal papillary mucinous neoplasms or mucinous cystic adenomas. In addition, resection is indicated for all tumors where preoperative diagnostic fails to provide an exact classification. Several different operative techniques are available. The treatment of choice depends on the localization of the tumor, its size and on whether there is evidence of malignant transformation. Partial duodenopancreatectomy is the oncological treatment of choice for tumors of the pancreatic head whereas for tumors of the pancreatic tail a left-sided pancreatectomy is appropriate. Middle pancreatectomy or duodenum-preserving resection of the pancreatic head is not a radical oncologic procedure. They should only be performed in cases of tumors without malignant potential. (orig.) [German] Die Indikationsstellung zur Resektion benigner Pankreastumoren ist gegeben, wenn es sich um einen symptomatischen Tumor handelt oder - bei einem Zufallsbefund - um einen Tumor mit Potenzial zur malignen Entartung. Dies besteht bei der Mehrzahl der benignen Pankreastumoren, insbesondere bei der intraduktalen papillaeren muzinoesen Neoplasie (IPMN) oder muzinoesen Zystadenomen. Operativer Abklaerung beduerfen auch Tumoren, die unter Ausschoepfung aller diagnostischer Moeglichkeiten nicht eindeutig klassifizierbar sind. An chirurgischen Therapieverfahren stehen verschiedene Techniken zur Verfuegung. Die Wahl des Verfahren haengt von der Groesse und Lokalisation des Tumors ab und von der Frage, ob eine maligne Entartung bereits stattgefunden hat. Das onkologisch korrekte Standardresektionsverfahren bei Tumoren des Pankreaskopfes ist die partielle Duodenopankreatektomie, bei Tumoren des Pankreasschwanzes die Pankreaslinksresektion. Eine segmentale Resektion des

  2. Möglichkeiten zur Verbesserung des Vitamin-D-Status der Bevölkerung

    Directory of Open Access Journals (Sweden)

    Brown J

    2011-01-01

    Full Text Available Vitamin D hat ein breites Einflussspektrum im menschlichen Körper und ein Vitamin- D-Mangel wird mit einer Vielzahl skelettaler und extraskelettaler chronischer Krankheiten in Verbindung gebracht. Aufgrund der ungünstigen geographischen Lage und der vorherrschenden Ernährungsgewohnheiten sind in Deutschland und vielen anderen europäischen Staaten die natürlichen Vitamin-D-Quellen Sonne und Nahrung zur Deckung des Vitamin-D-Bedarfs nicht ausreichend. Die 3 wesentlich zu unterscheidenden Strategien zur Linderung des Vitamin-DMangels sind: (1 staatlich geförderte Aufklärungskampagnen, (2 Vitamin-D-Supplementation und (3 Nahrungsmittelanreicherung. Da die genannten Strategien Vor- und Nachteile haben, könnte eine Kombination dieser Möglichkeiten zielführend sein. Die Basis sollte die Aufklärung der Bevölkerung bilden, indem die Mangelsituation und deren Gründe sowie Wege zur Lösung transparent gemacht werden. Die Grundversorgung der breiten Bevölkerungsmasse könnte durch mit Vitamin D angereicherte Lebensmittel sichergestellt und der Vitamin-D-Serumspiegel von Risikogruppen durch zusätzliche Supplementierung angehoben werden. Die Nahrungsmittelanreicherung geeigneter Lebensmittel wie beispielsweise Brot ist eine vielversprechende Maßnahme, um sicher und suffizient die Vitamin-D-Versorgung der deutschen Bevölkerung zu optimieren. Die Strategie der Vitamin-D-Nahrungsmittelanreicherung hat in der Vergangenheit eine Vielzahl von Ländern, wie etwa Finnland, Kanada oder die Vereinigten Staaten von Amerika, erfolgreich angewendet.

  3. Ultraschall der Finger zur Beurteilung struktureller peri- und postmenopausaler Knochenveränderungen

    Directory of Open Access Journals (Sweden)

    Boschitsch E

    2000-01-01

    Full Text Available Die phalangeale Ultrasonographie (PHUS ist eines der neueren Ultraschallverfahren, die in den vergangenen Jahren zur Einschätzung des Frakturrisikos entwickelt worden sind. Basierend auf der Transmission von Ultraschallwellen durch die proximalen Phalangen der Hand werden spezifische Parameter, wie die amplitudenabhängige Schallgeschwindigkeit (amplitude dependent speed of sound, ADSOS gemessen. In einer prospektiven offenen Studie wurde bei 338 peri- und postmenopausalen Frauen die PHUS anhand zweier für das Osteoporoserisiko bedeutender Veränderungen des Knochengewebes evaluiert und mit einer etablierten Methode zur Bestimmung der Knochendichte (bone mineral density, BMD, der dual energy X-ray absorptiometry (DEXA, in Beziehung gesetzt: Erstens wurden die vom Alter abhängigen Veränderungen und zweitens die durch eine Hormonersatztherapie (hormone replacement therapy, HRT hervorgerufenen Effekte geprüft. Zur Beurteilung altersabhängiger Knochenveränderungen wurde ADSOS mit BMD verglichen: Beide Parameter wiesen eine negative lineare Korrelation mit dem Alter der Patientinnen auf. Diese war für ADSOS besser als für BMD. Die Sensitivität von PHUS und DEXA für den Effekt einer HRT wurde durch den Vergleich einer Gruppe substituierter Frauen mit einer Kontrollgruppe geprüft: ein signifikanter Unterschied zwischen den Gruppen war für ADSOS, nicht aber für BMD festzustellen. Die schwache Korrelation zwischen ADSOS und BMD weist darauf hin, daß mit den beiden Techniken verschiedene Eigenschaften des Knochengewebes gemessen werden. BMD ist der klassische Parameter des Mineralgehalts, ADSOS hingegen scheint in erster Linie strukturelle Eigenschaften wiederzuspiegeln. Die gegebenen Daten sollten in weiterführenden Untersuchungen dahingehend überprüft werden, ob die kombinierte Anwendung von Knochendichtemessung (DEXA und Knochenultraschall (PHUS die Einschätzung des Frakturrisikos verbessern kann.

  4. Heat recovery in sewage sludge gasification systems; Waermerueckgewinnung bei Anlagen zur Klaerschlammvergasung

    Energy Technology Data Exchange (ETDEWEB)

    Lamp, P.; Reichel, A.; Ziegler, F. [Bayerisches Zentrum fuer Angewandte Energieforschung e.V. (ZAE Bayern), Abt. Energieumwandlung und -speicherung, Garching (Germany)

    1996-12-31

    Using the example of a pilot plant for sewage sludge gasification by the Noell conversion process, the potential for energy optimisation is described. The goals of development are twofold: First, the process stages must be optimized so as to minimize energy consumption and secondly, all options for energy use and energy must be utilized. (orig) [Deutsch] Anhand einer Pilotanlage zur Klaerschlammvergasung nach dem NOELL-Konversionsverfahren sollen Moeglichkeiten der energetischen Optimierung beispielhaft aufgezeigt werden. Ziel muss es sein, zum einen die Verfahrensschritte auf einen moeglichst geringen Energiebedarf hin zu optimieren und zum anderen Moeglichkeiten der Waermeverschiebung und -rueckgewinnung soweit wie moeglich auszunutzen. (orig)

  5. On the way to strategic management of public utilities; Auf dem Weg zur strategischen Unternehmensfuehrung

    Energy Technology Data Exchange (ETDEWEB)

    Mueller, C. [Inst. fuer Public Management, Berlin (Germany)

    2004-07-01

    The contribution presents the results of an investigation of strategy-defining processes and of the methods and tools of strategic management of public utilities in Germany. The data are based on a 2003 enquiry of 269 public utilities, each of which is a provider for a minimum of 30,000 inhabitants. (orig.) [German] Der Beitrag gibt einen Ueberblick ueber die Ergebnisse einer Untersuchung der strategiebildenden Prozesse und angewandten Methoden und Instrumente zur strategischen Unternehmensfuehrung in den Energieversorgungsunternehmen (EVU) Deutschlands. Grundlage hierfuer ist eine im Jahr 2003 durchgefuehrte Umfrage unter 269 EVU, wobei jedes EVU mindestens 30.000 Einwohner in seinem Versorgungsgebiet zaehlt. (orig.)

  6. Entwicklung eines selbstfahrenden Transport- und Antriebsfahrzeuges zur Unterstützung von Arbeiten in Weinbausteillagen

    OpenAIRE

    Hum, Sandor

    2013-01-01

    Der traditionelle Steillagenweinbau in Deutschland unterliegt seit längerem einer schweren Krise, da die Produktionskosten aufgrund des hohen Arbeitsaufwands in Form von Handarbeit gegenüber den Produktionskosten in Direktzuglagen ein Vielfaches übersteigen. Darüber hinaus ist es schwierig, noch motivierte und geeignete Arbeitskräfte für diese schwere und gefährliche Arbeit zu finden. Ansätze zur Mechanisierung der Steilhangbewirtschaftung gibt es bereits durch seilzuggeführte Steillagenme...

  7. Pulverisierung von hochviskosen zuckerhaltigen Flüssigkeiten zur Herstellung lagerfähiger Pulver

    OpenAIRE

    Haensel, Mirjam

    2013-01-01

    Zähe Flüssigkeiten (z.B. Malzextrakt) weisen in vielen Herstellungsverfahren ungünstige Eigenschaften aufgrund ihrer hohen Viskosität auf und erschweren den Verarbeitungsprozess. Im Rahmen dieser Arbeit werden verschiedene Sprühverfahren ohne und mit dem Einsatz von Trägerstoffen zur Erzeugung von Pulvern eingesetzt und hinsichtlich ihrer stofflichen Qualität sowie der Pulvereigenschaften verglichen. Als Ergebnis zeigte sich, dass mit dem Einsatz von Trägerstoffen stabile Pulver hergestellt w...

  8. Zur Interaktion von Genotyp und Ernährung bei Darmkrebs

    OpenAIRE

    Behrends, Thomas

    2013-01-01

    Ziel dieser Arbeit war es, sowohl die Auswirkungen einer veränderten Selenversorgung über die Nahrung als auch die Rolle des zentralen Transport- und Speicherproteins für Selen (Selenoprotein P, SepP) auf die intestinale Tumorigenese tierexperimentell zu untersuchen. Eine gestörte SepP-Expression, führte zur Ausbildung größerer Tumore. Durch eine Steigerung der Selenversorgung über die Nahrung eine signifikante Reduktion von Tumoranzahl und Gesamttumorfläche erzielt werden. Hierzu wurde den ...

  9. Sprechkompetenz im DaF-Unterricht an Slowenischen Gymnasien: von der Theorie zur Praxis

    Directory of Open Access Journals (Sweden)

    Andreja Retelj

    2016-12-01

    Full Text Available Es liegt auf der Hand, dass Fremdsprachenkenntnisse aufgrund der wachsenden Globalisierung stets an Bedeutung gewinnen. Über eine hochentwickelte Fremdsprachenkompetenz zu verfügen, ist in der heutigen Gesellschaft fast zur Selbstverständlichkeit geworden. Durch die wachsenden wirtschaftlichen Kontakte gewinnt vor allem die mündliche Kommunikation immer mehr an Bedeutung und die Erwartungen an Fremdsprachenlernende, in verschiedenen Sprachsituationen mündlich schnell und angemessen kommunizieren zu können, nehmen aus diesem Grund ständig zu.

  10. Zur Qualitätsausbildung und Qualitätserhaltung gartenbaulicher Produkte

    OpenAIRE

    Masoud, Alaa Abd El-Gaber Badawi

    2003-01-01

    Qualität wird nach ISO 8402 als Gesamtheit von Eigenschaften und Merkmalen eines Produktes, die sich auf deren Eignung zur Erfüllung festgelegter oder vorausgesetzter Erfordernisse beziehen, definiert. Produkte mit hohem Wassergehalt und großer Atmungsrate können erhebliche Qualitätsbeeinträchtigungen in der Nachernteperiode bis zum Verkauf bzw. Verzehr erleiden. Am Beispiel des Spargels, also junger und physiologisch sehr aktiver Pflanzensprosse, werden äußere und innere Qualitätsmerkmale ...

  11. Untersuchungen zur Konstruktvalidität des Verhaltens- und Erlebensinventars (VEI) an einer klinischen Stichprobe

    OpenAIRE

    Groves, Julia

    2009-01-01

    Die vorliegende Dissertation umfasst mehrere Studien zur Überprüfung der Konstruktvalidität des Verhaltens- und Erlebensinventars (VEI) an einer psychiatrischen Stichprobe. Das VEI ist die deutsche Adaption des Personality Assessment Inventory (PAI), ein komplett neu entwickelter klinischer Persönlichkeitsfragebogen (Morey, 1991). Die Adaption des PAI beinhaltete die sorgfältige Übersetzung der Items, die Überprüfung der Itemäquivalenz und einer abschließenden Überarbeitung der Items. Die end...

  12. Die muslimische Gemeinschaft Rumäniens: der Weg einer Elite zur marginalisierten Minderheit

    OpenAIRE

    Kahl, Thede

    2005-01-01

    Die Siedlungsgeschichte der Muslime auf dem Territorium des heutigen Rumäniens geht in das 13. Jahrhundert zurück. Durch verstärkte Einwanderung von Turkmenen und Tataren konnten sich im Laufe der folgenden Jahrhunderte Regionen mit muslimischer kultureller Dominanz ausbilden. Aufgrund der Kolonisierungspolitik der osmanischen Sultane im Laufe des 14.-16. Jahrhunderts erhöhte sich Zahl der Muslime ständig, da sie sowohl aus dem Norden des Schwarzen Meeres als auch aus dem Süden (Kleinasien) Z...

  13. The changes in hair growth pattern after autologous hair transplantation.

    Science.gov (United States)

    Lee, S H; Kim, D W; Jun, J B; Lee, S J; Kim, J C; Kim, N H

    1999-08-01

    Recently donor dominance has been emphasized in autologous hair transplantation while the influence of the recipient site has been considered negligible. In fact, there have been few studies that show this. This study was performed to examine the influence of the recipient site on transplanted hairs. A clinical study of 19 leprosy patients was performed. These patients had received single hair transplantation due to madarosis and were admitted to The Leprosy Mission, Jesus Hospital, Taegu, Korea, or had visited its outpatient clinic. In this study, the rate of growth, thickness of shaft, and graying rate between the transplanted eyebrow hair in the recipient site and scalp hair near the donor site were compared to observe the changes in the growth pattern of the hairs after transplantation. For most of the patients, the growth rate and graying rate of transplanted hairs were lower than those of hairs in the donor site. It seems that the recipient site may have an influence on the transplanted hairs. Further studies are needed, including clinical, histopathologic, and molecular biological methods.

  14. Quality Improvement Methodologies Increase Autologous Blood Product Administration

    Science.gov (United States)

    Hodge, Ashley B.; Preston, Thomas J.; Fitch, Jill A.; Harrison, Sheilah K.; Hersey, Diane K.; Nicol, Kathleen K.; Naguib, Aymen N.; McConnell, Patrick I.; Galantowicz, Mark

    2014-01-01

    Abstract: Whole blood from the heart–lung (bypass) machine may be processed through a cell salvaging device (i.e., cell saver [CS]) and subsequently administered to the patient during cardiac surgery. It was determined at our institution that CS volume was being discarded. A multidisciplinary team consisting of anesthesiologists, perfusionists, intensive care physicians, quality improvement (QI) professionals, and bedside nurses met to determine the challenges surrounding autologous blood delivery in its entirety. A review of cardiac surgery patients’ charts (n = 21) was conducted for analysis of CS waste. After identification of practices that were leading to CS waste, interventions were designed and implemented. Fishbone diagram, key driver diagram, Plan–Do–Study–Act (PDSA) cycles, and data collection forms were used throughout this QI process to track and guide progress regarding CS waste. Of patients under 6 kg (n = 5), 80% had wasted CS blood before interventions, whereas those patients larger than 36 kg (n = 8) had 25% wasted CS before interventions. Seventy-five percent of patients under 6 kg who had wasted CS blood received packed red blood cell transfusions in the cardiothoracic intensive care unit within 24 hours of their operation. After data collection and didactic education sessions (PDSA Cycle I), CS blood volume waste was reduced to 5% in all patients. Identification and analysis of the root cause followed by implementation of education, training, and management of change (PDSA Cycle II) resulted in successful use of 100% of all CS blood volume. PMID:24783313

  15. Micro-Autologous Fat Transplantation for Treating a Gummy Smile.

    Science.gov (United States)

    Huang, Shu-Hung; Huang, Yu-Hao; Lin, Yun-Nan; Lee, Su-Shin; Chou, Chih-Kang; Lin, Tsung-Ying; Takahashi, Hidenobu; Kuo, Yur-Ren; Lai, Chung-Sheng; Lin, Sin-Daw; Lin, Tsai-Ming

    2018-03-16

    A gummy smile is treated using a number of techniques, including botulinum toxin injection and various surgical interventions. Micro-autologous fat transplantation (MAFT) is a potentially advantageous alternative approach that has not been previously evaluated. This study sought to determine the long-term results of MAFT in patients with a gummy smile. Seven patients with gummy smiles were evaluated for MAFT treatment between October 2015 and April 2017. Centrifuged purified fat was micro-transplanted into the nasolabial groove, ergotrid, and upper lip areas using the MAFT-GUN while the patients were under total intravenous anesthesia. The mean age of the 7 patients was 31 years (range, 23-40 years). The mean operating time for MAFT was 52 minutes (range, 40-72 minutes), and the mean volume of fat delivered to the nasolabial groove, ergotrid, and upper lip was 16.1 mL. The mean decreases of gingival display in the right canine incisor, left canine incisor, right canine, and left canine teeth were 4.9, 4.6, 3.8, and 4.4 mm, respectively. The smiles of the 7 patients showed significant improvement at an average follow-up time of 12.9 months. Gummy smile treatment using MAFT is an effective, reliable, and relatively simple method, with high patient satisfaction and minimal risk of complications.

  16. Autologous Platelet-rich Plasma after Third Molar Surgery.

    Science.gov (United States)

    Gandevivala, Adil; Sangle, Amit; Shah, Dinesh; Tejnani, Avneesh; Sayyed, Aatif; Khutwad, Gaurav; Patel, Arpit Arunbhai

    2017-01-01

    The aim of this study is to compare the efficacy of autologous platelet-rich plasma (PRP) in the third molar impactions, with respect to: pain, swelling, healing, and periodontal status distal to the second molar in patients who need surgical removal of bilateral impacted mandibular third molars. Twenty-five patients of both sexes aged between 16 and 60 years who required bilateral surgical removal of their impacted third molars and met the inclusion criteria were included in the study. After surgical extraction of the third molar, primary closure was performed in the control group, whereas PRP was placed in the socket followed by primary closure in the case group. The outcome variables were pain, swelling, wound healing, and periodontal probe depth that were follow-up period of 2 months. Quantitative data are presented as mean. Statistical significance was checked by t -test. There was a difference in the pain (0.071) and facial swelling (0.184), reduction between test and control on day 3, but it was not found to be significant. Periodontal pocket depth (0.001) and wound healing (0.001) less in case group compared with the control group was found to be significant. The use of PRP lessens the severity of immediate postoperative sequelae and decreases preoperative pocket depth.

  17. Tissue engineering bone using autologous progenitor cells in the peritoneum.

    Science.gov (United States)

    Shen, Jinhui; Nair, Ashwin; Saxena, Ramesh; Zhang, Cheng Cheng; Borrelli, Joseph; Tang, Liping

    2014-01-01

    Despite intensive research efforts, there remains a need for novel methods to improve the ossification of scaffolds for bone tissue engineering. Based on a common phenomenon and known pathological conditions of peritoneal membrane ossification following peritoneal dialysis, we have explored the possibility of regenerating ossified tissue in the peritoneum. Interestingly, in addition to inflammatory cells, we discovered a large number of multipotent mesenchymal stem cells (MSCs) in the peritoneal lavage fluid from mice with peritoneal catheter implants. The osteogenic potential of these peritoneal progenitor cells was demonstrated by their ability to easily infiltrate decalcified bone implants, produce osteocalcin and form mineralized bone in 8 weeks. Additionally, when poly(l-lactic acid) scaffolds loaded with bone morphogenetic protein-2 (a known osteogenic differentiation agent) were implanted into the peritoneum, signs of osteogenesis were seen within 8 weeks of implantation. The results of this investigation support the concept that scaffolds containing BMP-2 can stimulate the formation of bone in the peritoneum via directed autologous stem and progenitor cell responses.

  18. Quality improvement methodologies increase autologous blood product administration.

    Science.gov (United States)

    Hodge, Ashley B; Preston, Thomas J; Fitch, Jill A; Harrison, Sheilah K; Hersey, Diane K; Nicol, Kathleen K; Naguib, Aymen N; McConnell, Patrick I; Galantowicz, Mark

    2014-03-01

    Whole blood from the heart-lung (bypass) machine may be processed through a cell salvaging device (i.e., cell saver [CS]) and subsequently administered to the patient during cardiac surgery. It was determined at our institution that CS volume was being discarded. A multidisciplinary team consisting of anesthesiologists, perfusionists, intensive care physicians, quality improvement (QI) professionals, and bedside nurses met to determine the challenges surrounding autologous blood delivery in its entirety. A review of cardiac surgery patients' charts (n = 21) was conducted for analysis of CS waste. After identification of practices that were leading to CS waste, interventions were designed and implemented. Fishbone diagram, key driver diagram, Plan-Do-Study-Act (PDSA) cycles, and data collection forms were used throughout this QI process to track and guide progress regarding CS waste. Of patients under 6 kg (n = 5), 80% had wasted CS blood before interventions, whereas those patients larger than 36 kg (n = 8) had 25% wasted CS before interventions. Seventy-five percent of patients under 6 kg who had wasted CS blood received packed red blood cell transfusions in the cardiothoracic intensive care unit within 24 hours of their operation. After data collection and didactic education sessions (PDSA Cycle I), CS blood volume waste was reduced to 5% in all patients. Identification and analysis of the root cause followed by implementation of education, training, and management of change (PDSA Cycle II) resulted in successful use of 100% of all CS blood volume.

  19. Rapid cell separation with minimal manipulation for autologous cell therapies

    Science.gov (United States)

    Smith, Alban J.; O'Rorke, Richard D.; Kale, Akshay; Rimsa, Roberts; Tomlinson, Matthew J.; Kirkham, Jennifer; Davies, A. Giles; Wälti, Christoph; Wood, Christopher D.

    2017-02-01

    The ability to isolate specific, viable cell populations from mixed ensembles with minimal manipulation and within intra-operative time would provide significant advantages for autologous, cell-based therapies in regenerative medicine. Current cell-enrichment technologies are either slow, lack specificity and/or require labelling. Thus a rapid, label-free separation technology that does not affect cell functionality, viability or phenotype is highly desirable. Here, we demonstrate separation of viable from non-viable human stromal cells using remote dielectrophoresis, in which an electric field is coupled into a microfluidic channel using shear-horizontal surface acoustic waves, producing an array of virtual electrodes within the channel. This allows high-throughput dielectrophoretic cell separation in high conductivity, physiological-like fluids, overcoming the limitations of conventional dielectrophoresis. We demonstrate viable/non-viable separation efficacy of >98% in pre-purified mesenchymal stromal cells, extracted from human dental pulp, with no adverse effects on cell viability, or on their subsequent osteogenic capabilities.

  20. Autologous miniature punch skin grafting in stable vitiligo

    Directory of Open Access Journals (Sweden)

    Savant S

    1992-01-01

    Full Text Available Autologous split thickness miniature punch skin grafting is one of the surgical modes of treatment of stable vitiligo. Out of 87 different sites, of stable vitiligo, occurring in 62 cases, (32 focal, 22 segmental and 8 generalised 75 sites showed total repigmentation with excellent cosmetic colour match. Out of the 62 cases, 46 cases who were treated postsurgically with PUVA therapy repigmented within 2 ½ to 3 months, 10 cases, who received no treatment postsurgically repigmented by 3 ½ to 6 months. In addition 6 cases in whom no treatment was given postsurgically had to be given PUVA therapy 3 months after surgery as there was poor repigmentation. The complications seen were graft rejection due to improper immobilization in 6 cases, graft rejection due to secondary infection in 1, contact allergic dermatitis to framycetin in 3, and reactivation of vitiligo in 2. Side effects seen were cobblestoning in 32, sinking pits in 12, variegated appearance in 4, and superficial scarring at donor site in all 62 cases.

  1. Sublabial Autologous Ear Cartilage Grafting for Increasing the Nasolabial Angle

    Directory of Open Access Journals (Sweden)

    Rajko Toncic

    2016-01-01

    Full Text Available BackgroundThe loss of nasal tip support is caused by many factors and eventually results in the collapse and eventual dropping of the nasal tip. This reduces the nasolabial (NL angle and negatively affects respiratory functions and one's appearance.MethodsThe aim of this retrospective study, which was conducted on 52 patients, was to present and popularize a simple and effective method for the reconstruction of a weakened columella by inserting an autologous ear cartilage graft using a sublabial approach.ResultsOf all the patients, three patients experienced transplant rejection. The period of follow-up observation was one to five years (mean, 27 months. The results were objectively evaluated by measuring the NL angle in standardized photos before and after the procedure at different time intervals over the follow-up period. We observed a significant increase of the NL angle (mean, 20°, and found these results to be durable over the long term. Of the 52 patients included in this study observed patients, three were dissatisfied (due to immediate infection and shifting of the strut, 28 were satisfied, and 21 were very satisfied.ConclusionsThe surgical method described here is simple and can be learned quickly. It has very good results with few complications, and is our method of choice for complex and serious cases seen in everyday rhinosurgical practice.

  2. Sleep disruption among cancer patients following autologous hematopoietic cell transplantation.

    Science.gov (United States)

    Nelson, Ashley M; Jim, Heather S L; Small, Brent J; Nishihori, Taiga; Gonzalez, Brian D; Cessna, Julie M; Hyland, Kelly A; Rumble, Meredith E; Jacobsen, Paul B

    2018-03-01

    Despite a high prevalence of sleep disruption among hematopoietic cell transplant (HCT) recipients, relatively little research has investigated its relationships with modifiable cognitive or behavioral factors or used actigraphy to characterize sleep disruption in this population. Autologous HCT recipients who were 6-18 months post transplant completed self-report measures of cancer-related distress, fear of cancer recurrence, dysfunctional sleep cognitions, and inhibitory sleep behaviors upon enrollment. Patients then wore an actigraph for 7 days and completed a self-report measure of sleep disruption on day 7 of the study. Among the 84 participants (age M = 60, 45% female), 41% reported clinically relevant sleep disruption. Examination of actigraph data confirmed that, on average, sleep was disrupted (wake after sleep onset M = 66 min) and sleep efficiency was less than recommended (sleep efficiency M = 78%). Cancer-related distress, fear of recurrence, dysfunctional sleep cognitions, and inhibitory sleep behaviors were related to self-reported sleep disruption (p valuesdisruption after transplant. Cancer-related distress, fear of recurrence, dysfunctional sleep cognitions, and maladaptive sleep behaviors are related to self-reported sleep disruption and should be considered targets for cognitive behavioral intervention in this population.

  3. Complement activated granulocytes can cause autologous tissue destruction in man

    Directory of Open Access Journals (Sweden)

    E. Löhde

    1992-01-01

    Full Text Available Activation of polymorphonuclear granulocytes (PMNs by C5a is thought to be important in the pathogenesis of multiple organ failure during sepsis and after trauma. In our experiment exposure of human PMNs to autologous zymosan activated plasma (ZAP leads to a rapid increase in chemiluminescence. Heating the ZAP at 56°C for 30 min did not alter the changes, while untreated plasma induced only baseline activity. The respiratory burst could be completely abolished by decomplementation and preincubation with rabbit antihuman C5a antibodies. Observation of human omentum using electron microscopy showed intravascular aggregation of PMNs, with capillary thrombosis and diapedesis of the cells through endothelial junctions 90 s after exposure to ZAP. PMNs caused disruption of connections between the mesothelial cells. After 4 min the mesothelium was completely destroyed, and connective tissue and fat cells exposed. Native plasma and minimum essential medium did not induce any morphological changes. These data support the concept that C5a activated PMNs can cause endothelial and mesothelial damage in man. Even though a causal relationship between anaphylatoxins and organ failure cannot be proved by these experiments C5a seems to be an important mediator in the pathogenesis of changes induced by severe sepsis and trauma in man.

  4. Long-term use and follow-up of autologous and homologous cartilage graft in rhinoplasty

    Directory of Open Access Journals (Sweden)

    Ghasemali Khorasani

    2016-05-01

    Full Text Available Background: Cartilage grafting is used in rhinoplasty and reconstructive surgeries. Autologous rib and nasal septum cartilage (auto graft is the preferred source of graft material in rhinoplasty, however, homologous cartilage (allograft has been extensively used to correct the nasal framework in nasal deformities. Autologous cartilage graft usage is restricted with complication of operation and limiting availability of tissue for extensive deformities. Alternatively, preserved costal cartilage allograft represents a readily available and easily contoured material. The current study was a formal systematic review of complications associated with autologous versus homologous cartilage grafting in rhinoplasty patients. Methods: In this cohort retrospective study, a total of 124 patients undergone primary or revision rhinoplasty using homologous or autologus grafts with postoperative follow-up ranging from 6 to 60 months were studied. The types of grafts and complications related to the grafts were evaluated. This included evaluation for warping, infection, resorption, mobility and fracture. Results: The total complications related to the cartilage grafts were 7 cases, which included 1 warped in auto graft group, three cases of graft displacement (two in allograft group and one in auto graft group and three fractures in allograft group. No infection and resorption was recorded. Complication rate (confidence interval 0.95 in autologous and homologous group were 1.25(0.4-3.88 and 2.08(0.78-5.55 in 1000 months follow up. There was no statistically significant difference between autologous and homologous group complications. Onset of complication in autologous and homologous group were 51.23(49.27-53.19 and 58.7(54.51-62.91 month respectively (P=0.81. Conclusion: The allograft cartilage has the advantage of avoiding donor-site scar. Moreover, it provides the same benefits as autologous costal cartilage with comparable complication rate. Therefore, it

  5. Uses of continuous measuring techniques for optimizing the operation of municipal sewage treatment plants; Einsatzmoeglichkeiten kontinuierlicher Messtechnik zur Betriebsoptimierung kommunaler Klaeranlagen

    Energy Technology Data Exchange (ETDEWEB)

    Wedi, D. [Vermicon AG, Muenchen/Braunschweig (Germany)

    1997-12-31

    Three different optimization measures are briefly discussed, each of which was carried out with the support of continuous measurements: efforts to enhance the sedimentation performance of a final sedimentation tank, investigations to establish the nitrification performance of an existing plant, and measurements permitting optimized parametrization of the complex control of a new sewage treatement plant. (orig./SR) [Deutsch] Es werden drei verschiedene Optimierungsmassnahmen kurz erlaeutert, die jeweils mit Unterstutzung kontinuierlicher Messungen erfolgten: Arbeiten zur Verbesserung der Sedimentationsleistung eines Nachklaerbeckens, Untersuchungen zur besseren Nachrechnung der Nitrifikationskapazitaet einer bestehenden Anlage und Messungen zur optimierten Parametrierung einer komplexeren Regelung einer neuen Klaeranlage. (orig./SR)

  6. Medien – Generationen – Wissen. Überlegungen zur medienpädagogischen Forschung – dargestellt am Beispiel der Frage nach dem Weltwissen globaler Mediengenerationen

    Directory of Open Access Journals (Sweden)

    Theo Hug

    2017-07-01

    Full Text Available Mit den aktuellen gesellschaftlichen, technologischen und politischen Entwicklungen haben sich neue Fragen und Aufgabenbereiche für die Medienpädagogik ergeben. Diese werden im vorliegenden Beitrag skizziert, wobei in Abgrenzung von traditionellen Auffassungen für ein weiteres Verständnis von Medienpädagogik argumentiert wird. Die Erweiterung des thematischen Horizonts wird anhand einer Pilotstudie zur Frage des Weltwissens von Mediengenerationen exemplarisch verdeutlicht. Abschließend werden einige Überlegungen zur Medienkompetenz, der Problematik diesbezüglicher Verkürzungen und deren Stellenwert im Lichte des „medial turn“ zur Diskussion gestellt.

  7. Enantioselektivität und Isotopendiskriminierung - Zur Analytik von 1,2-Propandiol und 4-Hydroxy-2,5-dimethyl-3(2H)-furanon (Furaneol)

    OpenAIRE

    Hartlieb, Ariane

    2009-01-01

    Aussagekräftige Methoden zur Authentizitätsbewertung sind unerlässlich, um so-wohl einen fairen Handel als auch das Vertrauen der Verbraucher in die Qualität von Lebensmitteln zu gewährleisten. Das Ziel dieser Arbeit war es, anhand der Parameter 'Enantioselektivität' und 'Iso-topendiskriminierung' Methoden zur Authentizitätskontrolle von Schaumweinen und Erdbeeren, beides Produkte mit einem vergleichsweise hohen Handelswert, zur Verfügung zu stellen. Um unmittelbare Anwendbarkeit in der Praxi...

  8. Model investigations on the possibilities of restoring the ozon layer. Final report; Modelluntersuchungen zur Moeglichkeit der Restauration der Ozonschicht. Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Roeth, E.P.; Reinhardt, H.; Zellner, R.

    2001-02-01

    Proposals from the literature how to restore the stratospheric ozone layer had been investigated in respect to the possibility of application. All investigated proposals are not applicable. (orig.) [German] Vorschlaege zur Restauration der stratosphaerischen Ozonschicht in der Literatur wurden auf ihre Anwendungsmoeglichkeiten hin untersucht. Alle Vorschlaege, die untersucht wurden, sind nicht durchfuehrbar. (orig.)

  9. [Heinz von zur Mühlen. Paul Johansen und die Sogenannten Undeutschen in Reval/Tallinn] / Paul Kaegbein

    Index Scriptorium Estoniae

    Kaegbein, Paul

    2008-01-01

    Arvustus: Heinz von zur Mühlen. Paul Johansen und die Sogenannten Undeutschen in Reval/Tallinn. - Nordosteuropa als Geschichtsregion. Helsinki : Aue-Stiftung :; Lübeck : Schmidt-Römhild, 2006. lk. 103-111. Paul Johanseni lähim töökaaslane kirjeldab teose "Deutsch und Undeutsch im mittelalterlichen und frühneuzeitlichen Reval" valmimist

  10. Von neuen Geschäftsideen zur gelebten Digitalisierung in Utility 4.0 - das Integrierte Geschäftsmodell

    Science.gov (United States)

    Doleski, Oliver D.

    Die Energiewirtschaft benötigt neue, digitale Geschäftsmodelle. Gegenwärtig folgt auf Liberalisierung und Energiewende die nächste Stufe einer weitreichenden Bereinigung des Versorgungsmarktes. Digitalisierung und Dezentralisierung sind heute in aller Munde und verlangen nach neuen Produkten und Dienstleistungen. Dabei wirken die immensen Herausforderungen einer digitalen Energiewelt wie Beschleuniger für die Transformation im Versorgungssektor und tragen damit zur breiten Etablierung von Utilities 4.0 bei. Dieser Entwicklungsprozess vollzieht sich mithilfe unterschiedlicher Methoden zur Realisierung neuer Geschäftsideen. Allerdings greifen die gängigen Konzepte zur Entwicklung von Geschäftsmodellen gerade im Hinblick auf die Berücksichtigung komplexer, unbeständiger Rahmenbedingungen und spezifischer Anforderungen der digitalen Energiewelt mitunter zu kurz. Vor diesem Hintergrund wird das auf dem ganzheitlichen St. Galler Management-Konzept beruhende Integrierte Geschäftsmodell iOcTen als geeignetes Instrumentarium zur Geschäftsmodellentwicklung vorgestellt. Neben der Modellbeschreibung unterstützt ein intuitiv verständlicher Leitfaden den Praktiker bei der Transformation vom klassischen Versorgungsunternehmen zum digitalen Energiedienstleistungsunternehmen.

  11. [Heinz von zur Mühlen. Das Bürgertum Fordert Blut. Ein Bolschewistiches Flugblatt von 1918] / Paul Kaegbein

    Index Scriptorium Estoniae

    Kaegbein, Paul

    2007-01-01

    Arvustus: Heinz von zur Mühlen. Das Bürgertum Fordert Blut. Ein Bolschewistiches Flugblatt von 1918. In : Ostseeprovinzen, baltische Staaten und das Nationale. Münster : LIT, 2005, lk. 403-414. 1918. aastast pärit lendlehest, mille käekirja järgi otsustades on kirjutanud Viktor Kingissepp

  12. Sports Activity After Reconstruction of Osteochondral Lesions of the Talus With Autologous Spongiosa Grafts and Autologous Matrix-Induced Chondrogenesis.

    Science.gov (United States)

    Wiewiorski, Martin; Werner, Lorenzo; Paul, Jochen; Anderson, Andrew E; Barg, Alexej; Valderrabano, Victor

    2016-10-01

    For the treatment of osteochondral lesions of the talus (OCLTs), autologous matrix-induced chondrogenesis (AMIC) is a safe 1-step procedure with good clinical and radiological results. However, data regarding postoperative sports activity after AMIC are limited. To identify significant factors influencing the rate of postoperative sports and recreational activities. Case series; Level of evidence, 4. The sports and recreational activities of 60 patients (mean age, 34.9 ± 11.5 years) undergoing the AMIC procedure were retrospectively analyzed at a mean of 46.9 ± 17.8 months (range, 24.5-87.0 months) postoperatively. The visual analog scale (VAS) for pain score, Tegner activity scale score, activity rating scale (ARS) score, and satisfaction with surgery outcomes were assessed. Corrective calcaneal osteotomy was performed in 38 of 60 (63.3%) patients. Ligament repair was performed in 41 of 60 (68.3%) patients. The mean VAS score improved significantly from 6.9 ± 1.6 points (range, 5-10 points) preoperatively to 2.3 ± 1.9 points (range, 0-6 points) at latest follow-up (P sports activity before the onset of symptoms became significantly lower at the time of surgery (from 95.0% to 53.3%; P sports frequency and the duration of sports activity was found postoperatively. Patients undergoing AMIC repair of an OCLT participate at a similar low postoperative sports and recreational activity level compared with the preoperative level. © 2016 The Author(s).

  13. Identifikationsverfahren zur Analyse von EEG-Signalen bei Epilepsie mit Reaktions-Diffusions Netzwerken

    Science.gov (United States)

    Gollas, F.; Tetzlaff, R.

    2007-06-01

    Partielle Differentialgleichungen des Reaktions-Diffusions-Typs beschreiben Phänomene wie Musterbildung, nichtlineare Wellenausbreitung und deterministisches Chaos und werden oft zur Untersuchung komplexer Vorgänge auf den Gebieten der Biologie, Chemie und Physik herangezogen. Zellulare Nichtlineare Netzwerke (CNN) sind eine räumliche Anordnung vergleichsweise einfacher dynamischer Systeme, die eine lokale Kopplung untereinander aufweisen. Durch eine Diskretisierung der Ortsvariablen können Reaktions-Diffusions-Gleichungen häufig auf CNN mit nichtlinearen Gewichtsfunktionen abgebildet werden. Die resultierenden Reaktions-Diffusions-CNN (RD-CNN) weisen dann in ihrer Dynamik näherungsweise gleiches Verhalten wie die zugrunde gelegten Reaktions-Diffusions-Systeme auf. Werden RD-CNN zur Identifikation neuronaler Strukturen anhand von EEG-Signalen herangezogen, so besteht die Möglichkeit festzustellen, ob das gefundene Netzwerk lokale Aktivität aufweist. Die von Chua eingeführte Theorie der lokalen Aktivität Chua (1998); Dogaru und Chua (1998) liefert eine notwendige Bedingung für das Auftreten von emergentem Verhalten in zellularen Netzwerken. Änderungen in den Parametern bestimmter RD-CNN könnten auf bevorstehende epileptische Anfälle hinweisen. In diesem Beitrag steht die Identifikation neuronaler Strukturen anhand von EEG-Signalen durch Reaktions-Diffusions-Netzwerke im Vordergrund der dargestellten Untersuchungen. In der Ergebnisdiskussion wird insbesondere auch die Frage nach einer geeigneten Netzwerkstruktur mit minimaler Komplexität behandelt.

  14. Diagnostic evaluation of rare lesions of the arteria poplitea; Zur Diagnostik seltener Erkrankungen der Arteria poplitea

    Energy Technology Data Exchange (ETDEWEB)

    Schmitt, R.; Christopoulos, G.; Froehner, S. [Inst. fuer Diagnostische und Interventionelle Radiologie (Germany); Binder, F.; Schweiger, H. [Abt. fuer Gefaesschirurgie der Herz- und Gefaessklinik GmbH Bad Neustadt an der Saale (Germany)

    2001-07-01

    Clinical and radiological findings of six patients are discussed who, due to a specific pathology of the arteria poplitea, developed claudicatio primarily in the lower leg. Applying MS-CT and MRI, the following lesions of the arteria poplitea wer found: bilateral aneurysm verum (1 case), unilateral aneurysm spurium (1 case), the arteria magna syndrome (1 case), entrapment syndrome (2 cases), and cystic adventitia degeneration. The outstanding diagnostic value of MR angiography combined with MR imaging is explained. Good knowledge of the specific pathology of the arteria poplitea is pre-requisite of efficient diagnostic evaluation, and prevention of thromboembolic complications, or ineffective therapy. (orig./CB) [German] Vorgestellt werden die klinischen und radiologischen Befunde von sechs Patienten, die auf dem Boden einer speziellen Pathologie der A. poplitea mit einer unterschenkelbetonten Claudicatio symptomatisch wurden. Mittels MS-CT und MR-Tomographie wurden an der A. poplitea die Befunde eines beidseitigen Aneurysma verum, eines unilateralen Aneurysma spurium, das Syndrom der Arteria magna, in zwei Faellen ein Entrapment-Syndrom sowie eine zystische Adventitiadegeneration nachgewiesen. Zur Diagnostik an der Poplitealarterie wird die herausragende Bedeutung der MR-Angiographie in Kombination mit der MR-Tomographie erlaeutert. Zur sicheren Diagnosefindung ist die Kenntnis der speziellen Krankheitsbilder der A. poplitea Voraussetzung, um thrombembolische Komplikationen im popliteokruralen Segment und ineffektive Therapiemassnahmen zu vermeiden. (orig.)

  15. Potenzial der mechanischen Unkrautkontrolle in Zuckerrüben zur Herbizideinsparung

    Directory of Open Access Journals (Sweden)

    Christoph Kunz

    2015-05-01

    Full Text Available Für die Unkrautbekämpfung in Zuckerrüben werden im frühen Wachstumsstadium der Pflanzen mehrmals großflächig Herbizide appliziert. In der vorliegenden Studie wurde untersucht, ob der Herbizideinsatz reduziert werden kann, indem Maßnahmen zur vorbeugenden, mechanischen und chemischen Unkrautkontrolle kombiniert werden. Die mechanische Unkrautbekämpfung zwischen den Reihen (inter-row wurde in Feldversuchen an 6 Standorten mit einer Bandapplikation zwischen den Pflanzen (intra-row kombiniert. An einem Standort wurde eine kamera- und eine GNSS-gesteuerte Reihenhacke eingesetzt. In den unbehandelten Parzellen (Kontrollversuch wurde eine Unkrautdichte bis zu 91 Pflanzen m-2 nachgewiesen. Bandapplikationen in Kombination mit dem Hacken zwischen den Reihen reduzierten den Herbizideinsatz um 50 bis 75 % im Vergleich zur ganzflächigen Herbizidapplikation. Die Effektivität der Unkrautbekämpfung betrug 72 % bei der konventionellen Herbizidanwendung, 87 % bei der Kombination von Reihenhacke und Bandapplikation, 78 % für die Reihenhacke mit Kamerasteuerung und 84 % für die Reihenhacke mit GNSS-RTK-Steuerung. Durch die Unkrautbekämpfung stieg der bereinigte Zuckerertrag (BZE – verglichen mit der unbehandelten Kontrollvariante – um 30 %. Die Kombination von mechanischer Unkrautkontrolle, Bandapplikation und präziser Reihenhacke als integrierte Unkrautbekämpfung führte zu guten Ergebnissen, d. h. der Herbizideinsatz konnte deutlich reduziert werden und die Unkrautbekämpfung wies eine hohe Wirksamkeit auf.

  16. Vertebroplasty in the treatment of back pain; Vertebroplastie zur Therapie des Rueckenschmerzes

    Energy Technology Data Exchange (ETDEWEB)

    Trumm, C.G.; Jakobs, T.F.; Zech, C.J.; Weber, C.; Reiser, M.F.; Hoffmann, R.T. [Klinikum Grosshadern der Ludwig-Maximilians-Universitaet, Institut fuer Klinische Radiologie, Muenchen (Germany)

    2006-06-15

    Beitrag sollen Indikationsstellung, periinterventionelle Bildgebung, Technik und klinische Ergebnisse der PVP dargestellt werden. Gemaess dem ''Interdisziplinaeren Konsensuspapier zur Vertebro- und Kyphoplastie'' der deutschen Fachgesellschaften sowie den Leitlinien der CIRSE aus dem Jahr 2005 werden die aktuellen Richtlinien zur Durchfuehrung der PVP erlaeutert. Die Ergebnisse einer eigenen Studie werden Komplikationsraten und klinischen Ergebnissen in der Literatur gegenuebergestellt. Die Indikation zur PVP besteht bei schmerzhaften osteoporotischen WKF und Wirbelkoerperosteolysen durch Metastasen und das multiple Myelom. Absolute Kontraindikationen sind v. a. asymptomatische WKF, eine Beschwerdelinderung unter medikamentoeser Therapie, therapierefraktaere Koagulopathien, Allergien gegen Zementbestandteile und aktive Infektionen. Zur Abschaetzung des Frakturalters, Ausschluss anderer Schmerzursachen und Beurteilung der Wirbelkoerperhinterkante sind vor der PVP eine MRT bzw. CT indiziert. Eine qualitativ hochwertige mono- oder biplanare Fluoroskopie - moeglichst in Kombination mit der CT(-Fluoroskopie) - sind bei der PVP erforderlich, um das Risiko von Zementleckagen zu minimieren. Bei 86-92% der Patienten wird durch die PVP eine deutliche Schmerzreduktion (mittlere Abnahme von 6,1 Punkten [VAS]) erreicht. Eine eigene Studie mit 58 behandelten Patienten (mittlerer Beobachtungszeitraum 323+/-99 Tage) zeigte in 77% der Faelle eine deutliche Beschwerdelinderung (-5,7 Punkte [VAS]). Die PVP ermoeglicht bei akutem und chronischem Rueckenschmerz durch osteoporotische WKF und tumorbedingte Osteolysen eine sichere und effektive minimalinvasive Therapie zur Stabilisierung und Schmerzreduktion. (orig.)

  17. Preeclampsia in autologous and oocyte donation pregnancy: is there a different pathophysiology?

    Science.gov (United States)

    Lashley, Lisa E E L O; Buurma, Aletta; Swings, Godelieve M J S; Eikmans, Michael; Anholts, Jacqueline D H; Bakker, Jaap A; Claas, Frans H J

    2015-06-01

    Oocyte donation (OD) is a specific method of artificial reproductive technology that is accompanied by a higher risk of preeclampsia during pregnancy. The pathophysiological mechanism underlying preeclampsia in OD pregnancies is thought to differ from preeclampsia in autologous pregnancies. As preeclampsia in autologous pregnancies is suggested to be associated with complement activation, we studied C4d deposition, circulating complement components and placental complement regulatory proteins in preeclamptic OD pregnancies. Women with uncomplicated and preeclamptic pregnancies after OD or spontaneous conception were selected. We stained the placentas for C4d, marker for complement activation, measured complement factors C1q, C3 and C4 in maternal sera and quantified the placental mRNA expression of complement regulatory proteins CD46, CD55 and CD59. A significantly (p preeclampsia compared with uncomplicated pregnancies, both OD and autologous. The level of complement factors in serum did not differ between the groups. Children born in the autologous preeclampsia group were significantly lower in birth weight (p preeclampsia pregnancies, there is excessive activation of complement in preeclamptic OD pregnancies. However, in contrast to autologous pregnancies this is not associated with counterbalancing upregulation of complement regulatory proteins. Furthermore, C4d deposition in OD pregnancies is not related to the severity of preeclampsia, suggesting another trigger or regulatory mechanism of placental C4d deposition in preeclamptic OD pregnancies. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  18. Autologous tenocyte therapy for experimental Achilles tendinopathy in a rabbit model.

    Science.gov (United States)

    Chen, Jimin; Yu, Qian; Wu, Bing; Lin, Zhen; Pavlos, Nathan J; Xu, Jiake; Ouyang, Hongwei; Wang, Allan; Zheng, Ming H

    2011-08-01

    Tendinopathy of the Achilles tendon is a chronic degenerative condition that frequently does not respond to treatment. In the current study, we propose that autologous tenocytes therapy (ATT) is effective in treating tendon degeneration in a collagenase-induced rabbit Achilles tendinopathy model. Chronic tendinopathy was created in the left Achilles tendon of 44 rabbits by an intratendonous injection of type I collagenase. Forty-two rabbits were randomly allocated into three groups of 14 and received control treatment; autologous tenocytes digested from tendon tissue; and autologous tenocytes digested from epitendineum tissue. For cell tracking in vivo, the remaining two animals were injected with autologous tenocytes labeled with a nano-scale super-paramagnetic iron oxide (Feridex). Rabbits were sacrificed at 4 and 8 weeks after the therapeutic injection, and tendon tissue was analyzed by histology, immunostaining, and biomechanical testing to evaluate tissue repair. Autologous tenocyte treatment improved tendon remodeling, histological outcomes, collagen content, and tensile strength of tendinopathic Achilles tendons. Injected tenocytes were integrated into tendon matrix and could be tracked up to 8 weeks in vivo. Immunohistochemistry showed that ATT improved type I collagen expression in repaired tendon but did not affect type III collagen and secreted protein, acidic and rich in cysteine expression. ATT may be a useful treatment of chronic Achilles tendinopathy.

  19. Rejuvenation of the periorbital complex with autologous fat transfer: current therapy.

    Science.gov (United States)

    Ciuci, Paul M; Obagi, Suzan

    2008-08-01

    This article examines the pathophysiology of periorbital aging and details a technique, autologous fat transfer, to restore a youthful appearance to the periorbital complex. The use of photographs taken of patients in their late teens or early 20s shows that the main changes of periorbital aging are consistent with volume loss more so than gravitational changes. This article reflects on the shortcomings of currently accepted surgical approaches to the rejuvenation of the periorbital region. After a detailed clinical evaluation of the periorbital region including overall facial aging, brow position, and eyelid laxity, patients underwent autologous fat transfer to the periorbital region. The technique consists of autologous fat harvesting using the senior author's modification of the Coleman technique to the upper brow and lower eyelid region. Detailed clinical evaluations of each subject, and comparisons to a preoperative youthful photograph of each, were made at 1 week, 6 weeks, and 6 months postoperatively. These evaluations included subjective clinical estimations of overall brow fullness, brow position, and upper and lower eyelid laxity; a judgment of the appearance of the supraorbital and infraorbital rim; appearance of a short lower eyelid length, and an assessment of the subject's convex facial profile. While maintaining a natural, "nonsurgical" appearance with this approach and this technique, the patient achieved a more youthful look after autologous fat transfer. Although there are still indications for brow lift and blepharoplasty in certain patients, neither procedure restores a youthful, rejuvenated appearance to the periorbital complex as well as autologous fat transfer.

  20. Autologous fibrin sealant (Vivostat®) in the neurosurgical practice: Part I: Intracranial surgical procedure

    Science.gov (United States)

    Graziano, Francesca; Certo, Francesco; Basile, Luigi; Maugeri, Rosario; Grasso, Giovanni; Meccio, Flavia; Ganau, Mario; Iacopino, Domenico G.

    2015-01-01

    Background: Hemorrhages, cerebrospinal fluid (CSF) fistula and infections are the most challenging postoperative complications in Neurosurgery. In this study, we report our preliminary results using a fully autologous fibrin sealant agent, the Vivostat® system, in achieving hemostasis and CSF leakage repair during cranio-cerebral procedures. Methods: From January 2012 to March 2014, 77 patients were studied prospectively and data were collected and analyzed. Autologous fibrin sealant, taken from patient's blood, was prepared with the Vivostat® system and applied on the resection bed or above the dura mater to achieve hemostasis and dural sealing. The surgical technique, time to bleeding control and associated complications were recorded. Results: A total of 79 neurosurgical procedures have been performed on 77 patients. In the majority of cases (98%) the same autologous fibrin glue provided rapid hemostasis and dural sealing. No patient developed allergic reactions or systemic complications in association with its application. There were no cases of cerebral hematoma, swelling, infection, or epileptic seizures after surgery whether in the immediate or in late period follow-up. Conclusions: In this preliminary study, the easy and direct application of autologous fibrin sealant agent helped in controlling cerebral bleeding and in providing prompt and efficient dural sealing with resolution of CSF leaks. Although the use of autologous fibrin glue seems to be safe, easy, and effective, further investigations are strongly recommended to quantify real advantages and potential limitations. PMID:25984391

  1. Qualitative assessment of blood washing with the continuous autologous transfusion system (CATS)

    Science.gov (United States)

    Walpoth, B H; Eggensperger, N; Walpoth-Aslan, B N; Neidhart, P; Lanz, M; Zehnder, R; Spaeth, P J; Kurt, G; Althaus, U

    1997-04-01

    A number of different blood-processing methods can be used at the end of cardiopulmonary bypass (CPB) to improve the quality of autologous blood. They include centrifugation, hemofiltration and cell-washing. They differ in processing time required, cost of disposables and the quality of the processed autologous blood product. The newly developed continuous auto-transfusion system (CATS: Fresenius AG, Bad Homburg) uses a continuous cell-washing method. In a prospective study, the oxygenator blood of 10 patients was processed at the end of cardiac surgery with CATS and the quality of autologous blood before and after processing was compared. The processing volumes and the time required were recorded. The concentrations and elimination rates of blood parameters and waste products such as activated coagulation and complement products were measured. At the end of CPB a mean volume of 1,010 +/- 174 ml diluted oxygenator blood was processed and concentrated to 310 +/- 88 ml in 11.0 +/- 2.2 mins. Cellular elements such as erythrocytes and leucocytes were mostly retained and their concentration showed a significant increase after processing (250% and 210% respectively; p plasma proteins. Likewise, all water soluble elements such as waste products are significantly lower in concentration after processing and, if calculated by quantity, they show a high elimination rate (> 93%). In conclusion, the continuous autologous transfusion system permits an automated, rapid and continuous processing of autologous blood yielding a standardised high quality erythrocyte concentrate.

  2. Regeneration of Tissues and Organs Using Autologous Cells

    Energy Technology Data Exchange (ETDEWEB)

    Anthony Atala, M D

    2012-10-11

    The proposed work aims to address three major challenges to the field of regenerative medicine: 1) the growth and expansion of regenerative cells outside the body in controlled in vitro environments, 2) supportive vascular supply for large tissue engineered constructs, and 3) interactive biomaterials that can orchestrate tissue development in vivo. Toward this goal, we have engaged a team of scientists with expertise in cell and molecular biology, physiology, biomaterials, controlled release, nanomaterials, tissue engineering, bioengineering, and clinical medicine to address all three challenges. This combination of resources, combined with the vast infrastructure of the WFIRM, have brought to bear on projects to discover and test new sources of autologous cells that can be used therapeutically, novel methods to improve vascular support for engineered tissues in vivo, and to develop intelligent biomaterials and bioreactor systems that interact favorably with stem and progenitor cells to drive tissue maturation. The Institute's ongoing programs are aimed at developing regenerative medicine technologies that employ a patient's own cells to help restore or replace tissue and organ function. This DOE program has provided a means to solve some of the vexing problems that are germane to many tissue engineering applications, regardless of tissue type or target disease. By providing new methods that are the underpinning of tissue engineering, this program facilitated advances that can be applied to conditions including heart disease, diabetes, renal failure, nerve damage, vascular disease, and cancer, to name a few. These types of conditions affect millions of Americans at a cost of more than $400 billion annually. Regenerative medicine holds the promise of harnessing the body's own power to heal itself. By addressing the fundamental challenges of this field in a comprehensive and focused fashion, this DOE program has opened new opportunities to treat

  3. Autologous Intravenous Mononuclear Stem Cell Therapy in Chronic Ischemic Stroke

    Directory of Open Access Journals (Sweden)

    Bhasin A

    2012-01-01

    Full Text Available Background: The regenerative potential of brain has led to emerging therapies that can cure clinico-motor deficits after neurological diseases. Bone marrow mononuclear cell therapy is a great hope to mankind as these cells are feasible, multipotent and aid in neurofunctional gains in Stroke patients. Aims: This study evaluates safety, feasibility and efficacy of autologous mononuclear (MNC stem cell transplantation in patients with chronic ischemic stroke (CIS using clinical scores and functional imaging (fMRI and DTI. Design: Non randomised controlled observational study Study: Twenty four (n=24 CIS patients were recruited with the inclusion criteria as: 3 months–2years of stroke onset, hand muscle power (MRC grade at least 2; Brunnstrom stage of recovery: II-IV; NIHSS of 4-15, comprehendible. Fugl Meyer, modified Barthel Index (mBI and functional imaging parameters were used for assessment at baseline, 8 weeks and at 24 weeks. Twelve patients were administered with mean 54.6 million cells intravenously followed by 8 weeks of physiotherapy. Twelve patients served as controls. All patients were followed up at 24 weeks. Outcomes: The laboratory and radiological outcome measures were within normal limits in MNC group. Only mBI showed statistically significant improvement at 24 weeks (p<0.05 whereas the mean FM, MRC, Ashworth tone scores in the MNC group were high as compared to control group. There was an increased number of cluster activation of Brodmann areas BA 4, BA 6 post stem cell infusion compared to controls indicating neural plasticity. Cell therapy is safe and feasible which may facilitate restoration of function in CIS.

  4. Arthroscopic autologous chondrocyte implantation in the ankle joint.

    Science.gov (United States)

    Giannini, Sandro; Buda, Roberto; Ruffilli, Alberto; Cavallo, Marco; Pagliazzi, Gherardo; Bulzamini, Maria Chiara; Desando, Giovanna; Luciani, Deianira; Vannini, Francesca

    2014-06-01

    Autologous chondrocyte implantation (ACI) is an established procedure in the ankle providing satisfactory results. The development of a completely arthroscopic ACI procedure in the ankle joint made the technique easier and reduced the morbidity. The purpose of this investigation was to report the clinical results of a series of patients who underwent arthroscopic ACI of the talus at a mean of 7 ± 1.2-year follow-up. Forty-six patients (mean age 31.4 ± 7.6) affected by osteochondral lesions of the talar dome (OLT) received arthroscopic ACI between 2001 and 2006. Patients were clinically evaluated using AOFAS score pre-operatively and at 12, 36 months and at final follow-up of 87.2 ± 14.5 months. The mean pre-operative AOFAS score was 57.2 ± 14.3. At the 12-month follow-up, the mean AOFAS score was 86.8 ± 13.4 (p = 0.0005); at 36 months after surgery, the mean score was 89.5 ± 13.4 (p = 0.0005); whereas at final follow-up of 87.2 ± 14.5 months it was 92.0 ± 11.2 (p = 0.0005). There were three failures. Histological and immunohistochemical evaluations of specimens harvested from failed implants generally showed several aspects of a fibro-cartilaginous tissue associated with some aspects of cartilage tissue remodelling as indicated by the presence of type II collagen expression. This study confirmed the ability of arthroscopic ACI to repair osteochondral lesions in the ankle joint with satisfactory clinical results after mid-term follow-up. IV, retrospective case series.

  5. Contralateral Autologous Corneal Transplantation Experience in Mexico City.

    Science.gov (United States)

    Perez-Balbuena, Ana L; Ancona-Lezama, David; Delgado-Pelayo, Sarai; Martinez, Jaime D

    2017-01-01

    The aim of this study is to expand the limited knowledge regarding autologous contralateral penetrating keratoplasty. We report the retrospective outcomes of patients who received autokeratoplasty and contralateral opaque corneas in the donor eye at a tertiary care ophthalmology hospital in Mexico City. Eleven patients received autokeratoplasty and contralateral opaque corneas in the donor eye at our center from 2010 to 2015. The mean patient age at the time of surgery was 58 years (range, 35-85 yrs), with 4 female and 7 male patients. There were no surgical or immediate postsurgical complications in the autokeratoplasty eye. However, 1 patient had expulsive hemorrhage in the sightless eye. Follow-up duration ranged from 11 to 65 months (mean, 26 mo). During follow-up, 3 of the autokeratoplasty procedures failed because of endothelial attenuation. Identified known risk factors for failure of the eye with visual potential included the presence of an Ahmed glaucoma drainage device in 7/11 patients (63%), history of glaucoma in 8/11 (72%), past heterologous penetrating keratoplasty in 2/11 (18%), Vogt-Koyanagi-Harada syndrome in 1/11 (9%), and 4-quadrant corneal vascularization in 1/11 (9%). Autokeratoplasty is a good choice in cases having high risk factors and when fresh corneal tissue is not available. This is the largest study describing outcomes of patients who underwent autokeratoplasty. This technique offers no risk of immune rejection and no need for immunosuppression treatment. This study reports a good prognosis in cases having high risk factors for failure.

  6. Cost analysis and quality of life assessment comparing patients undergoing autologous peripheral blood stem cell transplantation or autologous bone marrow transplantation for refractory or relapsed non-Hodgkin's lymphoma or Hodgkin's disease : a prospective randomised trial

    NARCIS (Netherlands)

    van Agthoven, M; Vellenga, E; Fibbe, WE; Kingma, T; Uyl-de Groot, CA

    The cost-effectiveness of autologous peripheral blood stem cell transplantation (PBSCT) compared with autologous bone marrow transplantation (ABMT) for refractory or relapsed non-Hodgkin's lymphoma (NHL) or Morbus Hodgkin (MH) was assessed. Costs were determined from the induction chemotherapy

  7. Detecting autologous blood transfusions: a comparison of three passport approaches and four blood markers

    DEFF Research Database (Denmark)

    Mørkeberg, J; Sharpe, K; Belhage, B

    2011-01-01

    Blood passport has been suggested as an indirect tool to detect various kinds of blood manipulations. Autologous blood transfusions are currently undetectable, and the objective of this study was to examine the sensitivities of different blood markers and blood passport approaches in order...... to determine the best approach to detect autologous blood transfusions. Twenty-nine subjects were transfused with either one (n=8) or three (n=21) bags of autologous blood. Hemoglobin concentration ([Hb]), percentage of reticulocytes (%ret) and hemoglobin mass (Hbmass) were measured 1 day before reinfusion...... and six times after reinfusion. The sensitivity and specificity of a novel marker, Hbmr (based on Hbmass and %ret), was evaluated together with [Hb], Hbmass and OFF-hr by different passport methods. Our novel Hbmr marker showed superior sensitivity in detecting the highest dosage of transfused blood...

  8. Gastrocnemius tendon strain in a dog treated with autologous mesenchymal stem cells and a custom orthosis.

    Science.gov (United States)

    Case, J Brad; Palmer, Ross; Valdes-Martinez, Alex; Egger, Erick L; Haussler, Kevin K

    2013-05-01

    To report clinical findings and outcome in a dog with gastrocnemius tendon strain treated with autologous mesenchymal stem cells and a custom orthosis. Clinical report. A 4-year-old spayed female Border Collie. Bone-marrow derived, autologous mesenchymal stem cells were transplanted into the tendon core lesion. A custom, progressive, dynamic orthosis was fit to the tarsus. Serial orthopedic examinations and ultrasonography as well as long-term force-plate gait analysis were utilized for follow up. Lameness subjectively resolved and peak vertical force increased from 43% to 92% of the contralateral pelvic limb. Serial ultrasonographic examinations revealed improved but incomplete restoration of normal linear fiber pattern of the gastrocnemius tendon. Findings suggest that autologous mesenchymal stem cell transplantation with custom, progressive, dynamic orthosis may be a viable, minimally invasive technique for treatment of calcaneal tendon injuries in dogs. © Copyright 2013 by The American College of Veterinary Surgeons.

  9. Autologous Fat Grafting as a Novel Approach to Parastomal Soft-tissue Volume Deficiencies

    Directory of Open Access Journals (Sweden)

    Robert C. Wu, MD

    2014-03-01

    Full Text Available Summary: The aim of this study is to describe a novel approach to revise maladaptive soft-tissue contour around an ileostomy. A patient with permanent ileostomy suffered from significant defects in soft-tissue contour due to scarring and wound contraction. He underwent autologous fat grafting to achieve sealing of his stoma appliance and improve cosmesis. Due to numerous surgeries, the stoma appliance would not seal and required daily appliance changes. The patient received autologous fat grafting to augment the contour around stoma. A complete fitting of stoma was achieved. The patient is satisfied with stoma sealing and is changing his stoma appliance every 5–7 days without skin excoriation. Autologous fat transfer is an effective approach to treat a subset of stoma patients with complex subcutaneous defects.

  10. Enrichment of autologous fat grafts with ex-vivo expanded adipose tissue-derived stem cells for graft survival

    DEFF Research Database (Denmark)

    Kølle, Stig-Frederik Trojahn; Fischer-Nielsen, Anne; Mathiasen, Anders Bruun

    2013-01-01

    Autologous fat grafting is increasingly used in reconstructive surgery. However, resorption rates ranging from 25% to 80% have been reported. Therefore, methods to increase graft viability are needed. Here, we report the results of a triple-blind, placebo-controlled trial to compare the survival ...... of fat grafts enriched with autologous adipose-derived stem cells (ASCs) versus non-enriched fat grafts....

  11. Is there evidence that barrier membranes prevent bone resorption in autologous bone grafts during the healing period? A systematic review

    NARCIS (Netherlands)

    Gielkens, Pepijn F. M.; Bos, Ruud R. M.; Raghoebar, Gerry M.; Stegenga, Boudewijn

    2007-01-01

    Introduction: Autologous bone is considered the "reference standard" for bone-grafting procedures. A barrier membrane covering an autologous bone graft (guided bone regeneration [GBR]) is expected to prevent graft resorption. Good clinical results have been reported for GBR, although potential

  12. Loss of quiescence and impaired function of CD34(+)/CD38(low) cells one year following autologous stem cell transplantation

    NARCIS (Netherlands)

    Woolthuis, Carolien M.; Brouwers-Vos, Annet Z.; Huls, Gerwin; de Wolf, Joost Th. M.; Schuringa, Jan Jacob; Vellenga, Edo

    2013-01-01

    Patients who have undergone autologous stem cell transplantation are subsequently more susceptible to chemotherapy-induced bone marrow toxicity. In the present study, bone marrow primitive progenitor cells were examined one year after autologous stem cell transplantation and compared with normal

  13. Autologous Chondrocyte Implantation "Sandwich" Technique Compared With Autologous Bone Grafting for Deep Osteochondral Lesions in the Knee.

    Science.gov (United States)

    Minas, Tom; Ogura, Takahiro; Headrick, Jeff; Bryant, Tim

    2018-02-01

    Treating symptomatic osteochondral defects is challenging, especially in young adults with deep (>8-10 mm) empty defects after osteochondritis dissecans (OCD) or collapsed condyles secondary to avascular necrosis (AVN). For this population, osteoarthritis (OA) is inevitable if articular congruence is not restored. To describe the autologous chondrocyte implantation (ACI) "sandwich" technique with autologous bone grafting (ABG) and compare it with ABG alone for restoration of the osteochondral unit. The midterm to long-term outcomes in patients after the treatment for OCD and AVN will be reported and compared. Cohort study; Level of evidence, 3. The outcomes for a consecutive cohort of 24 patients who underwent combined ABG with the ACI sandwich technique between 2001 and 2013 (ACI sandwich group) was compared with a historical control group of 17 consecutive patients who underwent ABG alone between 1995 and 2002 (ABG group) by a single surgeon for symptomatic deep (>8 mm) osteochondral lesions. Patients who were followed up with a minimum of 2 years were included in this study. The modified Cincinnati Knee Rating System, the Western Ontario and McMaster Universities Osteoarthritis Index, a visual analog scale (VAS), the Short Form-36, and a patient satisfaction survey were used to evaluate clinical outcomes. Survival analysis was performed using the Kaplan-Meier method, with no clinical improvement, graft failure, or conversion to prosthetic arthroplasty as the endpoint (failure). Kellgren-Lawrence (K-L) grading to assess OA progression was also performed. In the ABG group, 13 of 17 patients (76%) were available with a mean follow-up of 15.7 years postoperatively (range, 5-21 years). In the ACI sandwich group, all 24 patients were available with a mean follow-up of 7.8 years postoperatively (range, 2-15 years). No significant differences were observed between the groups in terms of age, sex, side of the operated knee, body mass index, lesion type, lesion size

  14. Evidence-based Public Health Nutrition : Entwicklung eines Konzepts für einen systematischen und standardisierten Ansatz zur Anwendung in Deutschland

    OpenAIRE

    Knorpp, Leonie Silvia

    2013-01-01

    Das Ziel der Arbeit war es, für den Anwendungsbereich Public Health Nutrition (PHN) ein evidenzbasiertes Konzept zu entwickeln, das eine transparente und nachvollziehbare Vorgehensweise zur systematischen und standardisierten Ableitung und Bewertung bevölkerungsbezogener Maßnahmen zur Prävention von ernährungsabhängigen Erkrankungen ermöglicht und die Vermittlung dieser Ergebnisse an politische Entscheidungsträger unterstützt. Mittels einer explorativen Literaturauswertung wurden existier...

  15. Survival and regeneration of deep-freeze preserved autologous cranial bones after cranioplasty.

    Science.gov (United States)

    Lu, Yi; Hui, Guozhen; Liu, Fengqiang; Wang, Zhengan; Tang, Yuming; Gao, Shuxing

    2012-04-01

    After decompressive craniectomy, a deep-freeze-preserved autologous cranial bone graft can be used for cranioplasty to avoid immunoreaction against an artificial patch material. Autologous cranial bone grafts not only have better physical properties, such as heat conduction, compared to artificial patch materials, but they also have the advantages of a lower medical cost and satisfactory physical flexibility. The discussion over (99)Tc(m)-MDP SPECT static cranial bone tomography in the diagnosis of survival and regeneration in deep-freeze preservation autologous cranial bones after cranioplasty is valuable. Objective. To investigate whether deep-freeze-preserved autologous cranial bone grafts could survive and regenerate after autologous reimplantation. The method of cranial bone preservation involved removing the cranial graft and sealing it in a double-layer sterile plastic bag under sterile surgical conditions. On the day of the cranioplasty operation, the cranial bone graft was disinfected by immersing it in 3% povidone-iodine for 30 minutes. At short-term (2 weeks), medium-term (3 months), and long-term (12 months) postoperative follow-up visits, (99)Tc(m)-MDP SPECT static cranial bone tomography was used to examine the reimplanted cranial bone. Results. There were no postoperative infections or seromas in all 16 cases. Two weeks following cranial bone graft reimplantation, the SPECT tomography showed some radioactivity uptake in the reimplanted cranial bone graft, which was lower than that in the cranial bone on the healthy side. At 3 months and 12 months after the operation, the radioactivity uptake in the reimplanted cranial bone graft was the same as that in the cranial bone on the healthy side. X-ray films showed blurred sutures in the reimplanted cranial bone graft at 12 months after surgery. Reimplanted deep-freeze-preserved autologous cranial bone can survive in the short term and regenerate in the medium and long terms.

  16. Phenotype and polarization of autologous T cells by biomaterial-treated dendritic cells.

    Science.gov (United States)

    Park, Jaehyung; Gerber, Michael H; Babensee, Julia E

    2015-01-01

    Given the central role of dendritic cells (DCs) in directing T-cell phenotypes, the ability of biomaterial-treated DCs to dictate autologous T-cell phenotype was investigated. In this study, we demonstrate that differentially biomaterial-treated DCs differentially directed autologous T-cell phenotype and polarization, depending on the biomaterial used to pretreat the DCs. Immature DCs (iDCs) were derived from human peripheral blood monocytes and treated with biomaterial films of alginate, agarose, chitosan, hyaluronic acid, or 75:25 poly(lactic-co-glycolic acid) (PLGA), followed by co-culture of these biomaterial-treated DCs and autologous T cells. When autologous T cells were co-cultured with DCs treated with biomaterial film/antigen (ovalbumin, OVA) combinations, different biomaterial films induced differential levels of T-cell marker (CD4, CD8, CD25, CD69) expression, as well as differential cytokine profiles [interferon (IFN)-γ, interleukin (IL)-12p70, IL-10, IL-4] in the polarization of T helper (Th) types. Dendritic cells treated with agarose films/OVA induced CD4+CD25+FoxP3+ (T regulatory cells) expression, comparable to untreated iDCs, on autologous T cells in the DC-T co-culture system. Furthermore, in this co-culture, agarose treatment induced release of IL-12p70 and IL-10 at higher levels as compared with DC treatment with other biomaterial films/OVA, suggesting Th1 and Th2 polarization, respectively. Dendritic cells treated with PLGA film/OVA treatment induced release of IFN-γ at higher levels compared with that observed for co-cultures with iDCs or DCs treated with all other biomaterial films. These results indicate that DC treatment with different biomaterial films has potential as a tool for immunomodulation by directing autologous T-cell responses. © 2014 Wiley Periodicals, Inc.

  17. Autologous Dendritic Cells Pulsed with Allogeneic Tumor Cell Lysate in Mesothelioma: From Mouse to Human.

    Science.gov (United States)

    Aerts, Joachim G J V; de Goeje, Pauline L; Cornelissen, Robin; Kaijen-Lambers, Margaretha E H; Bezemer, Koen; van der Leest, Cor H; Mahaweni, Niken M; Kunert, André; Eskens, Ferry A L M; Waasdorp, Cynthia; Braakman, Eric; van der Holt, Bronno; Vulto, Arnold G; Hendriks, Rudi W; Hegmans, Joost P J J; Hoogsteden, Henk C

    2018-02-15

    Purpose: Mesothelioma has been regarded as a nonimmunogenic tumor, which is also shown by the low response rates to treatments targeting the PD-1/PD-L1 axis. Previously, we demonstrated that autologous tumor lysate-pulsed dendritic cell (DC) immunotherapy increased T-cell response toward malignant mesothelioma. However, the use of autologous tumor material hampers implementation in large clinical trials, which might be overcome by using allogeneic tumor cell lines as tumor antigen source. The purpose of this study was to investigate whether allogeneic lysate-pulsed DC immunotherapy is effective in mice and safe in humans. Experimental Design: First, in two murine mesothelioma models, mice were treated with autologous DCs pulsed with either autologous or allogeneic tumor lysate or injected with PBS (negative control). Survival and tumor-directed T-cell responses of these mice were monitored. Results were taken forward in a first-in-human clinical trial, in which 9 patients were treated with 10, 25, or 50 million DCs per vaccination. DC vaccination consisted of autologous monocyte-derived DCs pulsed with tumor lysate from five mesothelioma cell lines. Results: In mice, allogeneic lysate-pulsed DC immunotherapy induced tumor-specific T cells and led to an increased survival, to a similar extent as DC immunotherapy with autologous tumor lysate. In the first-in-human clinical trial, no dose-limiting toxicities were established and radiographic responses were observed. Median PFS was 8.8 months [95% confidence interval (CI), 4.1-20.3] and median OS not reached (median follow-up = 22.8 months). Conclusions: DC immunotherapy with allogeneic tumor lysate is effective in mice and safe and feasible in humans. Clin Cancer Res; 24(4); 766-76. ©2017 AACR . ©2017 American Association for Cancer Research.

  18. Successful Treatment of Osgood–Schlatter Disease with Autologous-Conditioned Plasma in Two Patients

    Science.gov (United States)

    Danneberg, Dirk-Jonas

    2017-01-01

    Osgood–Schlatter Disease (OSD) is a painful, growth-related overuse condition of the tibial tuberosity, leading to inflammation of the patellar ligament at the tibial tuberosity. It primarily affects young adolescents, athletic population, and usually, resolves with age or skeletal maturity. Therapy is usually conservative, with surgery indicated in a minority of cases. For patients with treatment-resistant or refractory OSD, an alternative is the application of autologous platelet concentrate. Here, we describe two cases in which autologous-conditioned plasma therapy was used to treat OSD, and present the treatment protocol developed in our clinic. PMID:29270553

  19. Successful Treatment of Osgood-Schlatter Disease with Autologous-Conditioned Plasma in Two Patients.

    Science.gov (United States)

    Danneberg, Dirk-Jonas

    2017-09-01

    Osgood-Schlatter Disease (OSD) is a painful, growth-related overuse condition of the tibial tuberosity, leading to inflammation of the patellar ligament at the tibial tuberosity. It primarily affects young adolescents, athletic population, and usually, resolves with age or skeletal maturity. Therapy is usually conservative, with surgery indicated in a minority of cases. For patients with treatment-resistant or refractory OSD, an alternative is the application of autologous platelet concentrate. Here, we describe two cases in which autologous-conditioned plasma therapy was used to treat OSD, and present the treatment protocol developed in our clinic.

  20. Myeloid regeneration after whole body irradiation, autologous bone marrow transplantation, and treatment with an anabolic steroid

    International Nuclear Information System (INIS)

    Ambrus, C.M.; Ambrus, J.L.

    1975-01-01

    Stumptail monkeys (Macaca speciosa) received lethal whole-body radiation. Autologous bone marrow injection resulted in survival of the majority of the animals. Treatment with Deca-Durabolin, an anabolic steroid, caused more rapid recovery of colony-forming cell numbers in the bone marrow than in control animals. Both the Deca-Durabolin-treated and control groups were given autologous bone marrow transplantation. Anabolic steroid effect on transplanted bone marrow colony-forming cells may explain the increased rate of leukopoietic regeneration in anabolic steroid-treated animals as compared to controls

  1. Chondrocyte-seeded type I/III collagen membrane for autologous chondrocyte transplantation

    DEFF Research Database (Denmark)

    Niemeyer, Philipp; Lenz, Philipp; Kreuz, Peter C

    2010-01-01

    PURPOSE: We report the 2-year clinical results and identify prognostic factors in patients treated with autologous chondrocyte transplantation by use of a collagen membrane to seed the chondrocytes (ACT-CS). METHODS: This is a prospective study of 59 patients who were treated with ACT-CS and foll......PURPOSE: We report the 2-year clinical results and identify prognostic factors in patients treated with autologous chondrocyte transplantation by use of a collagen membrane to seed the chondrocytes (ACT-CS). METHODS: This is a prospective study of 59 patients who were treated with ACT...

  2. Successful autologous hematopoietic stem cell transplantation for a patient with rapidly progressive localized scleroderma.

    Science.gov (United States)

    Nair, Velu; Sharma, Ajay; Sharma, Sanjeevan; Das, Satyaranjan; Bhakuni, Darshan S; Narayanan, Krishnan; Nair, Vivek; Shankar, Subramanian

    2015-03-01

    Autologous hematopoietic stem cell transplant (HSCT) for rapidly progressive disease has not been reported in localized scleroderma. Our patient, a 16-year-old girl had an aggressive variant of localized scleroderma, mixed subtype (linear-generalized) with Parry Romberg syndrome, with no internal organ involvement, that was unresponsive to immunosuppressive therapy and was causing rapid disfigurement. She was administered autologous HSCT in June 2011 and has maintained drug-free remission with excellent functional status at almost 3.5 years of follow-up. © 2015 Asia Pacific League of Associations for Rheumatology and Wiley Publishing Asia Pty Ltd.

  3. Energy report for Bavaria, 1996/97. Data on the development of the Bavarian energy industry, with an energy balance; Energiebericht Bayern 1996/97. Daten zur Entwicklung der bayerischen Energiewirtschaft mit Energiebilanz

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-04-01

    The report presents the energy balance for Bavaria and provides information on: Bavarian energy industry; structure and development of primary and end-use energy consumption; situation and development of energy sources (electricity, gas, mineral oil, coal, district heating). (orig.) [Deutsch] Der Bericht enthaelt die Energiebilanz Bayern fuer das Berichtsjahr und bietet Informationen zur Energiewirtschaft in Bayern, zur Struktur und Entwicklung des Primaer- und Endenergieverbrauchs und zur Situation und Entwicklung bei den einzelnen Energietraegern (Elektrizitaet, Gas, Mineraloel, Kohle, Fernwaerme). (orig.)

  4. Waste heat recovery from clinker cooler for seawater desalination; Abwaermerueckgewinnung aus Klinkerkuehlern zur Meerwasserentsalzung

    Energy Technology Data Exchange (ETDEWEB)

    Muralidharan, V.M.; Swaminathan, R.; Lagu, S.R.; Kulkarni, R.V.

    2002-10-01

    The utilization of waste heat has been practised in cement works for many years. However, the clinker cooler exhaust air is being used for the first time in India to generate steam for a seawater desalination plant. The plant installed at the Gujarat cement works of Larsen and Toubro contributes to the production of 2040 m{sup 3}/day of demineralized water from seawater. Hot exhaust air from the clinker cooler at about 270 C is cooled to about 170 C in a waste heat recovery steam generator (WHRSG). The plant has now been in operation for more than three years and contributes to the production of water in an area in which water is a precious commodity. The plant was installed in an existing cement works, so a number of factors, such as operation of the clinker cooler, configuration and installation of the equipment, machinery and gas ducts, had to be taken into account during the design of the waste heat boiler. State-of-the-art techniques, such as pinch point analysis, finite element analysis and computational fluid dynamics, were used in the design. (orig.) [German] Die Abwaermenutzung in Zementwerken ist seit vielen Jahren gaengige Praxis. Zum ersten Mal wurde jetzt in Indien die Klinkerkuehlerabluft zur Dampferzeugung fuer eine Meerwasserentsalzungsanlage eingesetzt. Die im Zementwerk Gujarat von Larsen and Toubro installierte Anlage traegt zur Gewinnung von 2040 m{sup 3}/Tag destilliertem Wasser aus Meerwasser bei. Etwa 270 C heisse Abluft aus dem Klinkerkuehler wird in einem Abhitzekessel (WHRSG) auf ca. 170 C abgekuehlt. Die Anlage ist inzwischen seit mehr als drei Jahren in Betrieb und traegt zur Wassergewinnung in einem Gebiet bei, in dem Wasser ein kostbarer Rohstoff ist. Da die Anlage in einem vorhandenen Zementwerk errichtet wurde, gab es eine Reihe von Faktoren, wie z.B. Betrieb des Klinkerkuehlers, Anordnung und Montage der Ausruestungen, Maschinen und Gasleitungen, die bei der Konstruktion des Abhitzekessels zu beruecksichtigen waren. Fuer die

  5. A century of war. Anglo-American oil politics and the new world order; 2. ed.; Mit der Oelwaffe zur Weltmacht. Der Weg zur neuen Weltordnung

    Energy Technology Data Exchange (ETDEWEB)

    Engdahl, F.W.

    1993-12-31

    The book goes into the exiting history of petroleum which has become a weapon for the world domination in the hand of coldly calculation strategists. Already in World War One England was mainly interested in the oil fields. Petroleum flowed through the veins of the special relations between the United States and Great Britain since 1919. They combined big oil and big money in a factor of power which determines world history. The book shows how the two oil crises were arranged in Saltsjoebaden in Sweden at the beginning of 1973, how the present financial crisis results from that and how the new form of a ``colonialism by leasing`` developed. The author draws a plausible convincing picture of the history of our century without getting lost in ``right`` or ``left`` cliches. (orig./HS) [Deutsch] Das Buch erzaehlt die aufregende Geschichte des Erdoels, das in der Hand kalt kalkulierender Strategien zur Waffe um die Weltherrschaft wurde. Schon im Ersten Weltkrieg ging es England in erster Linie um die Erdoelfelder. Oel floss in den Adern der Sonderbeziehungen zwischen den USA und Grossbritannien seit 1919. Sie fassten das grosse Oel und das grosse Geld zu einem die Weltgeschichte bestimmenden Machtfaktor zusammen. Das Buch zeigt, wie die beiden Oelkrisen Anfang 1973 in Saltsjoebaden in Schweden verabredet wurden, wie daraus die gegenwaertige Finanzkrise erwuchs und die neue Form des ``Kolonialismus durch Leasing`` entstanden ist. Der Autor entwirft ein eineluchtendes, ueberzeugendes Bild von der Geschichte unseres Jahrhunderts, ohne in ``rechte`` oder ``linke`` Klischees zu verfallen. (orig./HS)

  6. Autologous Immune Enhancement Therapy for Cancer - Our experience since 2004

    Directory of Open Access Journals (Sweden)

    Hiroshi Terunuma

    2012-01-01

    Full Text Available Cancer, the major killer disease of the century requires a multi-pronged approach and among the latest modalities of treatments, Immunotherapy occupies a promising role. Immunotherapy for cancer was first started to be practised in the NIH and cell based immunotherapy for cancer is in practice for the past three decades. [1, 2] There are several literatures from various countries on the successful application of cell based Immunotherapies for various solid tumours and haematological malignancies. [3-8] Our team’s association with immune cells started when I was working on RNA transcriptome analysis to understand the immune system in HIV carriers which in turn required in vitro expansion of human Natural Killer (NK cells. [9] This led to the customization of protocols which has resulted in successful in vitro expansion, activation of NK cells and T cells for Immunotherapy. The purpose of Biotherapy institute of Japan (BIJ is to support research and clinical application of immune cells like NK cells, γδT cells, αβT cells, Cytotoxic T lymphocytes (CTL and Dendritic cells (DC for application as Autologous Immune Enhancement Therapy (AIET to fight against cancer. AIET using NK cells, CTLs, DCs etc have been administered for more than 5000 patients since 2004 till date by BIJ. Principle of AIET: For AIET using NK cells, the process involves separation of lymphocytes from the peripheral blood of the patient followed by selective NK cell expansion using the expansion kit (BINKIT, BIJ, JAPAN without feeder layers and then infusion of the expanded-activated NK cells. [10,11] As reports suggest that the activity of peripheral blood NK cells are lower in cancer patients compared to normal individuals [12] and as in vitro expansion of NK cells increases the cytotoxic ability 5 to 10 fold, [13] the NK cells are expanded in vivo and then infused to the patient in AIET. We are also working on combination immunotherapy using NK cells and CTLs and also NK

  7. Versuche zur Gewinnung von katalytischen Antikörpern zur Hydrolyse von Arylcarbamaten und Arylharnstoffen. (English Title: Attempts to produce catalytic antibodies for hydrolysis of arylcarbamates and arylureas)

    Science.gov (United States)

    Werner, Deljana

    2002-05-01

    Im Rahmen dieser Arbeit gelang es, katalytische Antikörper zur Hydrolyse von Benzylphenylcarbamaten sowie zahlreiche monoklonale Antikörper gegen Haptene herzustellen. Es wurden verschiedene Hapten-Protein-Konjugate unter Verwendung unterschiedlicher Kopplungsmethoden hergestellt und charakterisiert. Zur Generierung der hydrolytisch aktiven Antikörper wurden Inzuchtmäuse mit KLH-Konjugaten von 4 Übergangszustandsanaloga (ÜZA) immunisiert. Mit Hilfe der Hybridomtechnik wurden verschiedene monoklonale Antikörper gegen diese ÜZA gewonnen. Dabei wurden sowohl verschiedene Immunisierungsschemata als auch verschiedene Inzuchtmausstämme und Fusionstechniken verwendet. Insgesamt wurden 32 monoklonale Antikörper gegen die verwendeten ÜZA selektiert. Diese Antikörper wurden in groen Mengen hergestellt und gereinigt. Zum Nachweis der Antikörper-vermittelten Katalyse wurden verschiedene Methoden entwickelt und eingesetzt, darunter immunologische Nachweismethoden mit Anti-Substrat- und Anti-Produkt-Antikörpern und eine photometrische Methode mit Dimethylaminozimtaldehyd. Der Nachweis der hydrolytischen Aktivität gelang mit Hilfe eines Enzymsensors, basierend auf immobilisierter Tyrosinase. Die Antikörper N1-BC1-D11, N1-FA7-C4, N1-FA7-D12 und R3-LG2-F9 hydrolysierten die Benzylphenylcarbamate POCc18, POCc19 und Substanz 27. Der Nachweis der hydrolytischen Aktivität dieser Antikörper gelang auch mit Hilfe der HPLC. Der katalytische Antikörper N1-BC1-D11 wurde kinetisch und thermodynamisch untersucht. Es wurde eine Michaelis-Menten-Kinetik mit Km von 210 µM, vmax von 3 mM/min und kcat von 222 min-1 beobachtet. Diese Werte korrelieren mit den Werten der wenigen bekannten Diphenylcarbamat-spaltenden Abzyme. Die Beschleunigungsrate des Antikörpers N1-BC1-D11 betrug 10. Das ÜZA Hei3 hemmte die hydrolytische Aktivität. Dies beweist, dass die Hydrolyse in der Antigenbindungsstelle stattfindet. Weiter wurde zwischen der Antikörperkonzentration und der

  8. Low-dose autologous in vitro opsonized erythrocytes. Radioimmune method and autologous opsonized erythrocytes for refractory autoimmune thrombocytopenic purpura in adults

    Energy Technology Data Exchange (ETDEWEB)

    Ambriz, R.; Munoz, R.; Pizzuto, J.; Quintanar, E.; Morales, M.; Aviles, A.

    1987-01-01

    Adult patients with chronic autoimmune thrombocytopenic purpura (ATP), which proved refractory to various treatments, received a single dose of autologous in vitro opsonized erythrocytes with 100 micrograms of anti-D IgG. In 1983, 30 of these patients were treated with autologous erythrocytes that had been opsonized and labeled with 25 mCi (740 MBq) of technetium Tc 99m; this treatment was designated as the radioimmune method. Favorable responses were noted in 36% of patients so treated. In 1985, another group of 16 patients with refractory ATP received therapy with autologous opsonized erythrocytes (AOPE) and 55% of these patients showed favorable responses. Five (17%) of the patients treated using the radioimmune method attained a complete, long-term (greater than 35 months) remission of their ATP, and five (31%) of the patients treated using AOPE remained in complete remission over 270 days after cessation of therapy. Major complications were not seen. We concluded that the interaction of macrophages with low-dose AOPE is a successful therapeutic approach in ATP refractory to standard treatment.

  9. Total balance of biogenic fuels for thermal uses; Ganzheitliche Bilanzierung verschiedener biogener Festbrennstoffe zur thermischen Nutzung

    Energy Technology Data Exchange (ETDEWEB)

    Becher, S.; Kaltschmitt, M. [Stuttgart Univ. (Germany). Inst. fuer Energiewirtschaft und Rationelle Energieanwendung (IER)

    1996-12-31

    In this situation of unfavourable energy price levels, the use of biogenic fuels for power supply can be recommended only if it serves to reduce environmental pollution. Against this background and on the basis of a primary energy balance, the authors attempted a total balance of selected enfironmental effects (global heating and acidification potential) of biomass use as compared to fossil fuel combustion. (orig) [Deutsch] ie Nutzung biogener Festbrennstoffe zur Energienachfragedeckung ist bei dem gegenwaertigen unguenstigen Energiepreisniveau nur dann zu rechtfertigen, wenn es durch die Biomassenutzung zu einer Reduzierung der energiebedingten Umwelteffekte kommt. Vor disem Hintergrund werden ausgehend von der Primaerenergiebilanz ausgewaehlte Umwelteffekte (d.h. das Treibhaus- und das Versauerungspotential) einer Biomassenutzung im Vergleich zu einer Nutzung fossiler Energietraeger ganzheitlich bilanziert. Die wesentlichen Ergebnisse werden zusammengefasst und interpretiert. (orig)

  10. Zur Rekonstruktion einer Typologie jugendlichen Medienhandelns gemäß dem Leitbild der Triangulation

    Directory of Open Access Journals (Sweden)

    Klaus Peter Treumann

    2017-09-01

    Full Text Available Die im Folgenden dargestellten Ergebnisse sind im Rahmen des von der DFG geförderten Forschungsprojekts „Eine Untersuchung zum Mediennutzungsverhalten 12- bis 20-Jähriger und zur Entwicklung von Medienkompetenz im Jugendalter“ entstanden, das gemeinsam von Klaus Peter Treumann, Uwe Sander und Dorothee Meister geleitet wird. Das Forschungsprojekt untersucht das Medienhandeln Jugendlicher sowohl hinsichtlich Neuer als auch alter Medien. Zum einen fragen wir dabei nach den Ausprägungen von Medienkompetenz in verschiedenen Dimensionen und zum anderen konzentrieren wir uns auf die Entwicklung einer empirisch fundierten Typologie jugendlichen Medienhandelns. Methodologisch ist die Untersuchung an dem Leitbild der Triangulation orientiert und kombiniert qualitative und quantitative Zugänge zum Forschungsfeld in Form von Gruppendiskussionen, leitfadengestützten Einzelinterviews und einer Repräsentativerhebung.

  11. Simulator to evaluate and optimise driver assistance systems; Ein Simulator zur Bewertung und Optimierung von Fahrerassistenzsystemen

    Energy Technology Data Exchange (ETDEWEB)

    Locher, J. [Hella KG Hueck and Co., Paderborn (Germany); Kleinkes, M. [Hella KG Hueck and Co., Lippstadt (Germany)

    2003-07-01

    For a short time now, the L-LAB (Light-LABoratory), a joint research facility of Hella KG and the University of Paderborn, has had a simulator available which allows the evaluation of driver assistance systems and the optimisation of man-machine interfaces. It is an interactive night driver simulator, which can be used to visualise any type of headlamp beam pattern true to the original. A Smart is used as the input medium; image projection is via a 3-channel high-performance beamer projection system onto large screens. Alongside synthetic roads, there is also a digitalised course available, approx. 10 km long, the original of which is actually used by Hella as a proving ground. Road safety indicators can be recorded directly and indirectly. For example, obstacles which suddenly appear force drivers to apply the brakes. The stopping distance required can be interpreted as a direct indicator for safety. In addition, an eye tracking system is used to record the eye movements of the test subjects, which allows additional road safety indicators to be derived. Comparative investigations between the digitalised proving ground and reality allow validation of the simulator. This year, a comprehensive study will be made to optimise the man-machine interface in connection with infrared vision systems in vehicles. The advantages of simulation are not only in the economical implementation possibilities and control of the variables, but also the fact that properties of driver assistance systems can be evaluated which are not even technically possible yet in reality. The use of the simulator developed here is not limited to tests connected with the visibility of obstacles, however. Rather, it allows the investigation and evaluation of a large range of potential driver assistance systems. (orig.) [German] Dem L-LAB (Light-LABoratory), einer gemeinsamen Forschungseinrichtung der Universitaet Paderborn und der Firma Hella KG in Lippstadt, steht seit kurzer Zeit ein Simulator zur

  12. Vertebroplastie – eine neue Therapieform zur Schmerzbehandlung von Wirbelkörperfrakturen

    Directory of Open Access Journals (Sweden)

    Gschwendtner M

    2003-01-01

    Full Text Available Wirbelkörperfrakturen sind meist sehr schmerzhaft, schränken die Leistungsfähigkeit ein, vermindern stark die Lebensqualität und dadurch das Selbstwertgefühl. Ursachen für diese schmerzhaften Wirbelkörperfrakturen können Osteoporose, Myelome, Metastasen und Wirbelkörperhämangiome sein. Die herkömmliche Therapie ist konservativ (Analgetika, Bettruhe, Mieder, chirurgische Maßnahmen erfolgen nur bei Instabilität und Kompressionssymptomen. Die perkutane Vertebroplastie unter Röntgenkontrolle (Durchleuchtung und Computertomographie stellt eine alternative Therapieform zur raschen Schmerztherapie dar. Im folgenden wird ein Überblick über die Entwicklung der Methode, Patientenselektion, Technik, Ergebnisse und Komplikationen der perkutanen Vertebroplastie gegeben.

  13. Konsensus zur Anwendung der "Tension-free Vaginal Tape" (TVT Operation bei der weiblichen Belastungsinkontinenz

    Directory of Open Access Journals (Sweden)

    Hanzal E

    2003-01-01

    Full Text Available Unter der Patronanz der Medizinischen Gesellschaft für Inkontinenzhilfe Österreich wurde im Juni 2002 in Wien die 2. Auflage eines Österreichischen Tension-free Vaginal Tape (TVT Konsensus-Meetings abgehalten. TVT ist eine neue Operationstechnik zur Behandlung der weiblichen Stressharninkontinenz, die seit 1998 in Österreich eingesetzt wird. Im Rahmen des Treffens, an dem Experten aus den Fachbereichen Urologie und Gynäkologie teilnahmen, wurde die vorhandene Fachliteratur analysiert und ein Konsensus für die präoperative Abklärung, Durchführung und postoperative Verlaufskontrolle als Basis für eine laufende Qualitätsverbesserung des Verfahrens erarbeitet, dessen Ergebnis in dem vorliegenden Papier als Leitlinie zusammengefaßt ist.

  14. Didaktische Konzeption von Serious Games: Zur Verknüpfung von Spiel- und Lernangeboten

    Directory of Open Access Journals (Sweden)

    Michael Kerres

    2009-08-01

    Full Text Available Serious Games versuchen Spielen mit Lernen zu verbinden. Der Beitrag problematisiert die Machbarkeit einer solchen Verknüpfung in digitalen Welten und diskutiert verschiedene didaktische Konzepte einer Nutzbarmachung digitaler Spiele für das Lernen und Lehren. Ausgehend von einer Analyse des Erlernens von Spielen werden drei didaktische Konzepte von Serious Games im Hinblick auf ihre didaktischen Potenziale untersucht: (1 der unmittelbare Transfer von Wissen, das im Spiel erworben wird (keine zusätzliche Didaktisierung, (2 Didaktisierung durch Einbettung des Spiels in einer Lernsituation oder (3 Didaktisierung durch Einbettung von Lernaufgaben in ein Spiel. Ergebnisse zur Nutzung von Spielen, bei denen Lernaufgaben in eine Spielwelt eingebettet sind, zeigen, dass Personen die Aufnahme von Wissen in einem expliziten Lernmodus eher zu reduzieren versuchen, um möglichst zügig wieder in den Spielmodus zu wechseln. Diskutiert werden die Konsequenzen dieser Problematik für die didaktische Konzeption von Serious Games.

  15. Investigations of pollutant migration using lysimeters; Untersuchungen zur Schadstoff-Migration in Lysimetern

    Energy Technology Data Exchange (ETDEWEB)

    Klotz, D. (ed.)

    2002-07-01

    The third GSF Lysimeter Workshop focused on pollutant migration in underground strata, i.e. the dispersion of water constituents as referred to water transport on the basis of ideal tracer investigations. [German] Der am 29./30.04.2002 stattgefundene Workshop 'Untersuchungen zur Schadstoffmigration in Lysimetern' war der dritte GSF-Lysimeterworkshop nach denen in den Jahren 1998 und 2000 veranstalteten. Nachdem in den Jahren 1998 und 2000 der Wassertransport und die Bestimmung der Sickerwassergeschwindigkeit in Lysimetern im Mittelpunkt standen, war 2002 die Schadstoffmigration im Untergrund der Schwerpunkt der Vortraege und Poster. Unter Migration wird das Ausbreitungsverhalten von Wasserinhalts-(Fremd-, Schad-)Stoffen bezogen auf den Wassertransport, bestimmt mit idealen Tracern, verstanden. (orig.)

  16. Die „Leipziger schule”. Zur geschichte der kirchenmusik Im 20. jahrhundert

    Directory of Open Access Journals (Sweden)

    Loos Helmut

    2013-01-01

    Full Text Available Auf die Initiative Karls Straubes ging die Gründung des Instituts für Kirchenmusik am Leipziger Konservatorium im Jahre 1921 zurück. Zusammen mit seinem Meisterschüler Günther Ramin gestaltete er über lange Jahre hinweg die Kirchenmusik in St. Thomas. Nach dem Ende des Ersten Weltkriegs gewann die Bach-Bewegung für den Protestantismus eine zentrale Bedeutung, zumal sich Leipzig als richtungsweisendes Zentrum profilierte und mit der „Ära Straube-Ramin” eine „Leipziger Schule” ausbildete. Obwohl Straube und Ramin nicht selbst komponierten, war insbesondere ihr Wirken am Konservatorium der Musik zu Leipzig wesentlich für die Bildung der „Leipziger Schule” als Erneuerungsbewegung evangelischer Kirchenmusik. In dem Artikel wird das Schicksal dieser Bestrebungen und ihrer Protagonisten während der katastrophalen ökonomischen Umstände und des anwachsenden Nationalisozialismus analysiert.

  17. Autologous bone marrow transplantation following chemotherapy and irradiation in dogs with spontaneous lymphomas

    International Nuclear Information System (INIS)

    Bowles, C.A.; Bull, M.; McCormick, K.; Kadin, M.; Lucas, D.

    1980-01-01

    Thirty dogs with spontaneous lymphomas were administered two to six cycles of chemotherapy and were randomized into 3 groups to receive 800 rads of total body irradiation and autologous bone marrow transplantation. Of 10 dogs irradiated after chemotherapy-induced remission and infused with remission marrow (group 1), 8 (80%) had successful grafts and experienced remissions lasting 62 to 1024 days. Of 9 dogs irradiated during remission and infused with remission marrow mixed with autologous tumor cells (group 2), 6 (66%) had remission lasting 15 to 45 days. Eleven dogs with progressive tumor growth (relapse) following chemotherapy were irradiated and infused with remission marrow (group 3). Tumor remission lasting 39 to 350 days was observed in 5 dogs (45%) in this group, and 6 dogs died in less than 30 days. Dogs in groups 1 to 3 had median survival times of 216, 60, and 45 days, respectively. The prolonged survival times for dogs in group 1 compared to dogs in groups 2 and 3 suggest that protocols involving irradiation and autologous marrow grafting in this model would be most effective when these protocols are applied to animals having a minimum tumor burden at the time of irradiation and when the grafting is done with tumor-free autologous marrow

  18. beta-TCP Versus Autologous Bone for Repair of Alveolar Clefts in a Goat Model.

    NARCIS (Netherlands)

    Ruiter, A. de; Meijer, G.J.; Dormaar, T.; Janssen, N.; Bilt, A. van der; Slootweg, P.J.; Bruijn, J. de; Rijn, L. van; Koole, R.A.

    2011-01-01

    Objective : The aim of this study in goats was to test the hypothesis that a novel synthetic bone substitute beta tricalcium phosphate (beta-TCP) can work as well as autologous bone harvested from the iliac crest for grafting and repair of alveolar clefts. Design : Ten adult Dutch milk goats ( Capra

  19. Experimental results and clinical impact of using autologous rectus fascia sheath for vascular replacement

    NARCIS (Netherlands)

    Kobori, Laszlo; Nemeth, Tibor; Nagy, Peter; Dallos, Gabor; Sotonyi, Peter; Fehervari, Imre; Nemes, Balazs; Gorog, Denes; Patonai, Attila; Monostory, Katalin; Doros, Attila; Sarvary, Enikoe; Fazakas, Janos; Gerlei, Zsuzsanna; Benkoe, Tamas; Piros, Laszlo; Jaray, Jeno; De Jong, Koert P.

    Vascular complications are major causes of graft failure in liver transplantation. The use of different vascular grafts is common but the results are controversial. The aim of this study was to create an 'ideal' arterial interponate for vascular replacements in the clinical field. An autologous,

  20. Transplantation of autologously derived mitochondria protects the heart from ischemia-reperfusion injury

    Science.gov (United States)

    Masuzawa, Akihiro; Black, Kendra M.; Pacak, Christina A.; Ericsson, Maria; Barnett, Reanne J.; Drumm, Ciara; Seth, Pankaj; Bloch, Donald B.; Levitsky, Sidney; Cowan, Douglas B.

    2013-01-01

    Mitochondrial damage and dysfunction occur during ischemia and modulate cardiac function and cell survival significantly during reperfusion. We hypothesized that transplantation of autologously derived mitochondria immediately prior to reperfusion would ameliorate these effects. New Zealand White rabbits were used for regional ischemia (RI), which was achieved by temporarily snaring the left anterior descending artery for 30 min. Following 29 min of RI, autologously derived mitochondria (RI-mitochondria; 9.7 ± 1.7 × 106/ml) or vehicle alone (RI-vehicle) were injected directly into the RI zone, and the hearts were allowed to recover for 4 wk. Mitochondrial transplantation decreased (P mitochondria (7.9 ± 2.9%) compared with RI-vehicle (34.2 ± 3.3%, P mitochondria hearts returned to normal contraction within 10 min after reperfusion was started; however, RI-vehicle hearts showed persistent hypokinesia in the RI zone at 4 wk of recovery. Electrocardiogram and optical mapping studies showed that no arrhythmia was associated with autologously derived mitochondrial transplantation. In vivo and in vitro studies show that the transplanted mitochondria are evident in the interstitial spaces and are internalized by cardiomyocytes 2–8 h after transplantation. The transplanted mitochondria enhanced oxygen consumption, high-energy phosphate synthesis, and the induction of cytokine mediators and proteomic pathways that are important in preserving myocardial energetics, cell viability, and enhanced post-infarct cardiac function. Transplantation of autologously derived mitochondria provides a novel technique to protect the heart from ischemia-reperfusion injury. PMID:23355340

  1. [Construction of a capsular tissue-engineered ureteral stent seeded with autologous urothelial cells].

    Science.gov (United States)

    Tan, Haisong; Fu, Weijun; Li, Jianqiang; Wang, Zhongxin; Li, Gang; Ma, Xin; Dong, Jun; Gao, Jiangping; Wang, Xiaoxiong; Zhang, Xu

    2013-01-01

    To investigate the feasibility of constructing a capsular poly L-lactic acid (PLLA) ureteral stent seeded with autologous urothelial cells using tissue engineering methods. The capsular ureteral stent was constructed by subcutaneously embedding PLLA ureteral stent in the back of beagles for 3 weeks to induce the formation of connective tissue on the surfaces. After decellularization of the stent, the expanded autologous urothelial cells were seeded on the stent. The surface structure and cell adhesion of the stent were observed using HE staining, scanning electron microscope (SEM) and immunocytochemical staining. MTT assay was used to evaluate urothelial cell proliferation on the capsular PLLA ureteral stent and on circumferential small intestinal submucosa graft. HE staining and VIII factor immunohistochemistry revealed numerous capillaries in the connective tissue encapsulating the stent without obvious local inflammatory response. The results of SEM and immunocytochemical staining showed that the capsule contained rich collagenic fibers forming three-dimensional structures, and the seeded autologous urothelial cells could adhere and well aligned on the surface. MTT assay showed normal growth of the cells on the stent as compared with the cells grown on circumferential small intestinal submucosa graft. The capsular PLLA ureteral stent allows adhesion and proliferation of autologous urothelial cells and shows a potential in applications of constructing tissue-engineered ureter.

  2. Specific Factors Influence the Success of Autologous and Allogeneic Hematopoietic Stem Cell Transplantation

    Directory of Open Access Journals (Sweden)

    Thissiane L. Gonçalves

    2009-01-01

    Full Text Available Successful hematopoietic stem cell transplantation (HSCT, both autologous and allogeneic, requires a rapid and durable engraftment, with neutrophil (>500/µL and platelet (>20,000/µL reconstitution. Factors influencing engraftment after autologous or allogeneic HSCT were investigated in 65 patients: 25 autologous peripheral stem cell transplantation (PBSCT and 40 allogeneic bone marrow transplantation (BMT patients. The major factor affecting engraftment was the graft source for HSCT. Neutrophil and platelet recovery were more rapid in autologous PBSCT than in allogeneic BMT [neutrophil occurring in median on day 10.00 (09.00/11.00 and 19.00 (16.00/23.00 and platelet on day 11.00 (10.00/13.00 and 21.00 (18.00/25.00, respectively; p < 0.0001]. The type of disease also affected engraftment, where multiple myeloma (MM and lymphoma showed faster engraftment when compared with leukemia, syndrome myelodysplastic (SMD and aplastic anemia (AA and MM presented the best overall survival (OS in a period of 12 months. Other factors included the drug used in the conditioning regimen (CR, where CBV, melphalan (M-200 and FluCy showed faster engraftment and M-200 presented the best OS, in a period of 12 months and age, where 50–59 years demonstrated faster engraftment. Sex did not influence neutrophil and platelet recovery.

  3. Evidences of autologous fat grafting for the treatment of keloids and hypertrophic scars

    Directory of Open Access Journals (Sweden)

    VINÍCIUS ZOLEZI DA SILVA

    Full Text Available SUMMARY Introduction Since the 1980s, the use of autologous fat grafting has been growing in plastic surgery. Recently, this procedure has come to be used as a treatment for keloids and hypertrophic scars mainly due to the lack of satisfactory results with other techniques. So far, however, it lacks more consistent scientific evidence to recommend its use. The aim of this study was to review the current state of autologous fat grafting for the treatment of keloids and hypertrophic scars, their benefits and scientific evidences in the literature. Method A review in the Pubmed database was performed using the keywords “fat grafting and scar”, “fat grafting and keloid scar” and “fat grafting and hypertrophic scar.” Inclusion criteria were articles written in English and published in the last 10 years, resulting in 15 studies. Results These articles indicate that autologous fat grafting carried out at sites with pathological scars leads to a reduction of the fibrosis and pain, an increased range of movement in areas of scar contraction, an increase in their flexibility, resulting in a better quality of scars. Conclusion So far, evidences suggest that autologous fat grafting for the treatment of keloids and hypertrophic scars is associated with a better quality of scars, leading to esthetic and functional benefits. However, this review has limitations and these findings should be treated with reservations, since they mostly came from studies with low levels of evidence.

  4. No positive effect of autologous platelet gel after total knee arthroplasty

    NARCIS (Netherlands)

    Peerbooms, Joost C.; de Wolf, Gideon S.; Colaris, Joost W.; Bruijn, Daniël J.; Verhaar, Jan A. N.

    2009-01-01

    Activated platelets release a cocktail of growth factors, some of which are thought to stimulate repair. We investigated whether the use of autologous platelet gel (PG) in total knee arthroplasty (TKA) would improve wound healing and knee function, and reduce blood loss and the use of analgesics.

  5. Autologous stem cell transplantation in treatment of aggressive non-Hodgkin's lymphoma

    NARCIS (Netherlands)

    Kluin-Nelemans, Hanneke

    2002-01-01

    There is no doubt that autologous stem cell transplantation is useful for patients with relapsed aggressive non-Hodgkin's lymphoma if they are responsive to the chemotherapy given before the transplantation. A small subset of patients with primary refractory disease still profits from this high dose

  6. Comparison of upfront tandem autologous-allogeneic transplantation versus reduced intensity allogeneic transplantation for multiple myeloma

    NARCIS (Netherlands)

    Sahebi, F.; Iacobelli, S.; Biezen, A.V.; Volin, L.; Dreger, P.; Michallet, M.; Ljungman, P.T.; Witte, T.J. de; Henseler, A.; Schaap, N.P.M.; Lopez-Corral, L.; Poire, X.; Passweg, J.; Hamljadi, R.M.; Thomas, S.H.; Schonland, S.; Gahrton, G.; Morris, C.; Kroger, N.; Garderet, L.

    2015-01-01

    We performed a retrospective analysis of the European Group for Blood and Marrow Transplantation database comparing the outcomes of multiple myeloma patients who received tandem autologous followed by allogeneic PSCT (auto-allo) with the outcomes of patients who underwent a reduced intensity

  7. Hemolytic uremic syndrome after high dose chemotherapy with autologous stem cell support

    NARCIS (Netherlands)

    van der Lelie, H.; Baars, J. W.; Rodenhuis, S.; Van Dijk, M. A.; de Glas-Vos, C. W.; Thomas, B. L.; van Oers, R. H.; von dem Borne, A. E.

    1995-01-01

    BACKGROUND: Chemotherapy intensification may lead to new forms of toxicity such as hemolytic uremic syndrome. METHODS: Three patients are described who developed this complication 4 to 6 months after high dose chemotherapy followed by autologous stem cell support. The literature on this subject is

  8. Severe encephalopathy after high-dose chemotherapy with autologous stem cell support for brain tumours

    NARCIS (Netherlands)

    van den Berkmortel, F.; Gidding, C.; de Kanter, M.; Punt, C. J. A.

    2006-01-01

    Recurrent medulloblastoma carries a poor prognosis. Long-term survival has been obtained with high-dose chemotherapy with autologous stem cell transplantation and secondary irradiation. A 21-year-old woman with recurrent medulloblastoma after previous chemotherapy and radiotherapy is presented. The

  9. Socket grafting with the use of autologous bone: an experimental study in the dog.

    Science.gov (United States)

    Araújo, Mauricio G; Lindhe, Jan

    2011-01-01

    studies in humans and animals have shown that following tooth removal (loss), the alveolar ridge becomes markedly reduced. Attempts made to counteract such ridge diminution by installing implants in the fresh extraction sockets were not successful, while socket grafting with anorganic bovine bone mineral prevented ridge contraction. to examine whether grafting of the alveolar socket with the use of chips of autologous bone may allow ridge preservation following tooth extraction. in five beagle dogs, the distal roots of the third and fourth mandibular premolars were removed. The sockets in the right or the left jaw quadrant were grafted with either anorganic bovine bone or with chips of autologous bone harvested from the buccal bone plate. After 3 months of healing, biopsies of the experimental sites were sampled, prepared for buccal-lingual ground sections and examined with respect to size and composition. it was observed that the majority of the autologous bone chips during healing had been resorbed and that the graft apparently did not interfere with socket healing or processes that resulted in ridge resorption. autologous bone chips placed in the fresh extraction socket will (i) neither stimulate nor retard new bone formation and (ii) not prevent ridge resorption that occurs during healing following tooth extraction.

  10. Effects of Autologous Fat and ASCs on Swine Hypertrophic Burn Scars: A Multimodal Quantitative Analysis

    Directory of Open Access Journals (Sweden)

    Scott J. Rapp, MD

    2017-11-01

    Conclusion:. Early results suggest that autologous fat and/or ASCs may improve healing of hypertrophic scarring by altering the cellular and structural components during wound remodeling up to 20 weeks after injury. This may have beneficial applications in early treatment of large or cosmetically sensitive immature burn scars.

  11. Autologous graft-versus-host disease induction in advanced breast cancer: role of peripheral bloodprogenitor cells

    NARCIS (Netherlands)

    Wall, E. van der; Horn, T.; Bright, E.; Passos-Coehlo, J-L.; Bond, S.; Clarke, B.; Altomonte, V.; McIntyre, K.; Vogelsang, G.; Noga, S.J.; Davis, J.M.; Thomassen, J.; Ohly, K.V.; Lee, S.M.; Fetting, J.; Armstrong, D.K.; Davidson, N.E.; Hess, A.D.; Kennedy, M.J.

    2000-01-01

    The purpose of the present study was to investigate the impact of the use of peripheral blood progenitor cells (PBPCs) on the induction of autologous graft-versus-host disease (GVHD) in patients with advanced breast cancer. 14 women with stage IIIB and 36 women with stage IV breast cancer received

  12. Up-front autologous stem-cell transplantation in peripheral T-cell lymphoma

    DEFF Research Database (Denmark)

    d'Amore, Francesco; Relander, Thomas; Lauritzsen, Grete F

    2012-01-01

    Systemic peripheral T-cell lymphomas (PTCLs) respond poorly to conventional therapy. To evaluate the efficacy of a dose-dense approach consolidated by up-front high-dose chemotherapy (HDT) and autologous stem-cell transplantation (ASCT) in PTCL, the Nordic Lymphoma Group (NLG) conducted a large...

  13. Treatment of Recurrent Corneal Epithelial Defect by Autologous Serum Eye Drop

    Directory of Open Access Journals (Sweden)

    Hossein Mohammad Rabei

    2015-02-01

    Full Text Available Background: The aim of the present study was to evaluate the efficacy of autologous serum eye drop in treatment of recurrent corneal epithelial defect.Materials and Methods: Fourteen patients with recurrent corneal epithelial defect were studied. Autologous serum was prepared from the patients and diluted in 20% normal saline. The patients were instructed to use the autologous serum every six hours. Patients were followed for a mean period of 18 months.Results: Four males (28.6% and 10 females (71.4% entered the study. Four patients stopped the treatment after three months with complete satisfaction from treatment. Patients reported a reduction in frequency and severity of attacks 4.6±2 weeks after the start of treatment. The mean number of attacks before the procedure was 7.6±0.9 per year which was reduced to 2.2±0.9 per year after treatment (p<0.001. The main side effects in patients were eye pruritus and redness which were well tolerated by patients.Conclusion: Autologous serum application seems to be a safe and effective method to treat recurrent corneal epithelial defect.

  14. Reaming debris as a novel source of autologous bone to enhance healing of bone defects

    NARCIS (Netherlands)

    Bakker, A.D.; Kroeze, R.J.; Korstjens, C.; Kleine, R.H.; Frolke, J.P.M.; Klein-Nulend, J.

    2011-01-01

    Reaming debris is formed when bone defects are stabilized with an intramedullary nail, and contains viable osteoblast-like cells and growth factors, and might thus act as a natural osteoinductive scaffold. The advantage of using reaming debris over stem cells or autologous bone for healing bone

  15. Rituximab purging and/or maintenance in patients undergoing autologous transplantation for relapsed follicular lymphoma

    DEFF Research Database (Denmark)

    Pettengell, Ruth; Schmitz, Norbert; Gisselbrecht, Christian

    2013-01-01

    The objective of this randomized trial was to assess the efficacy and safety of rituximab as in vivo purging before transplantation and as maintenance treatment immediately after high-dose chemotherapy and autologous stem-cell transplantation (HDC-ASCT) in patients with relapsed follicular lymphoma...

  16. Repair of a large congenital frontal bone defect with autologous exchange cranioplasty.

    Science.gov (United States)

    Ropper, Alexander E; Rogers, Gary F; Ridgway, Emily B; Proctor, Mark R

    2010-11-01

    The authors report the case of a large idiopathic frontal bone defect and concomitant sagittal synostosis corrected by autologous exchange cranioplasty by using a corticocancellous bone graft and cranial vault expansion. An otherwise healthy, developmentally normal 6-year-old girl presented to our clinic with a large frontal bone defect. The osseous defect was midline and inferior to the coronal sutures, and the underlying dura was slightly tense. She had no signs or symptoms of increased intracranial pressure, and her head circumference and cephalic index were normal. Imaging demonstrated fusion of the sagittal synostosis. The defect was repaired using full-thickness autologous bone harvested from the bilateral parietal regions, which were widened using barrel-stave osteotomies to reduce pressure on the graft site in the setting of sagittal synostosis and mild cranial narrowing. The donor sites were covered with autologous particulate bone graft harvested from the endocortical surface of the grafted segments and the ectocortical surface of the intact parietal bones. The donor and recipient sites healed. Imaging revealed that the particulate bone healed with a thickness similar to the surrounding bone. This bony defect is analogous to parietal foramina and may have a similar etiopathogenesis. The technique of autologous exchange cranioplasty using corticocancellous particulate bone graft provides a simple and reliable method to repair large structural calvarial defects.

  17. Reaming debris as a novel source of autologous bone to enhance healing of bone defects

    NARCIS (Netherlands)

    Bakker, Astrid D.; Kroeze, Robert Jan; Korstjens, Clara; de Kleine, Ruben H.; Frolke, Jan Paul M.; Klein-Nulend, Jenneke

    Reaming debris is formed when bone defects are stabilized with an intramedullary nail, and contains viable osteoblast-like cells and growth factors, and might thus act as a natural osteoinductive scaffold. The advantage of using reaming debris over stem cells or autologous bone for healing bone

  18. Intrathecal application of autologous bone marrow cell preparations in parkinsonian syndromes

    DEFF Research Database (Denmark)

    Storch, Alexander; Csoti, Ilona; Eggert, Karla

    2012-01-01

    A growing number of patients is treated with intrathecal application of autologous bone marrow cells (aBMCs), but clinical data are completely lacking in movement disorders. We provide first clinical data on efficacy and safety of this highly experimental treatment approach in parkinsonian...

  19. Treatment of midshaft clavicular nonunion with plate fixation and autologous bone grafting

    DEFF Research Database (Denmark)

    Olsen, Bo Sanderhoff; Vaesel, M T; Søjbjerg, Jens Ole

    1995-01-01

    , and one had a failure. Thirteen of 16 patients were satisfied with the cosmetic outcome, assessing their cosmetic result as either good or excellent. Rigid plate fixation and restoration of clavicular length with autologous cancellous bone graft is recommended for the treatment of symptomatic clavicular...

  20. Multiple congenitally missing teeth: treatment outcome with autologous transplantation and orthodontic space closure.

    Science.gov (United States)

    Fiorentino, Giuseppe; Vecchione, Pietro

    2007-11-01

    Treatment for patients with congenitally missing teeth can be challenging. The treatment options include retaining the deciduous teeth, extracting the deciduous teeth and allowing the space to close spontaneously, implant replacement, autotransplantation, prosthetic replacement, and orthodontic space closure. Autologous transplantation and space closure with orthodontic appliances are demonstrated in this case report.

  1. Use of autologous grafts in the treatment of acquired penile curvature: An experience of 33 cases

    Directory of Open Access Journals (Sweden)

    Abdul Rouf Khawaja

    2016-01-01

    Conclusion: Tunical lengthening procedures by autologous free grafts represents a safe and reproducible technique. A good preoperative erectile function is required for tunical lengthening procedure. Temporalis fascia graft is thin, tough membrane and effective graft for PD with good cosmetic and functional results.

  2. Autologous stem cell transplantation versus alternative allogeneic donor transplants in adult acute leukemias.

    Science.gov (United States)

    Claude Gorin, Norbert

    2016-04-01

    The availability of alternative sources of stem cells including most recently T-replete haploidentical marrow or peripheral blood, and the increasing use of reduced-intensity conditioning (RIC), renders feasible an allogeneic transplant to almost all patients with acute leukemia up to 70 years of age. Autologous stem cell transplantation (ASCT) for consolidation of complete remission (CR), however, offers in some circumstances an alternative option. Although associated with a higher relapse rate, autologous transplant benefits from a lower non-relapse mortality, the absence of graft-versus-host disease (GVHD), and a better quality of life for long-term survivors. The recent use of intravenous busulfan (IVBU) with high-dose melphalan, better monitoring of minimal residual disease (MRD), and maintenance therapy post autografting bring new interest. Few retrospective studies compared the outcome following alternative donor versus autologous transplants for remission consolidation. Genoidentical and phenoidentical allogeneic stem cell transplantations are undisputed gold standards, but there are no data showing the superiority of alternative allogeneic donor over autologous transplantation, at the time of undetectable MRD, in patients with good- and intermediate-1 risk acute myelocytic leukemia (AML) in first complete remission (CR1), acute promyelocytic leukemia in second complete remission (CR2), and Philadelphia chromosome-positive (Ph(+)) acute lymphocytic leukemia (ALL). Copyright © 2016. Published by Elsevier Inc.

  3. Investigation of an autologous blood treatment strategy for temporomandibular joint hypermobility in a pig model

    Czech Academy of Sciences Publication Activity Database

    Štembírek, Jan; Matalová, Eva; Buchtová, Marcela; Machoň, V.; Míšek, Ivan

    2013-01-01

    Roč. 42, č. 3 (2013), s. 369-375 ISSN 0901-5027 Grant - others:GA MŠk(CZ) 1M0528 Institutional support: RVO:67985904 Keywords : temporomandibular joint * pig * autologous blood * hypermobility Subject RIV: FF - HEENT, Dentistry Impact factor: 1.359, year: 2013

  4. Lysis of fresh human solid tumors by autologous lymphocytes activated in vitro with lectins

    International Nuclear Information System (INIS)

    Mazumder, A.; Grimm, E.A.; Zhang, H.Z.; Rosenberg, S.A.

    1982-01-01

    Human peripheral blood lymphocytes (PBL), obtained from patients with a variety of cancers, were incubated in vitro with phytohemagglutinin, concanavalin A, and crude or lectin-free T-cell growth factors. The lectin-activated PBL of nine patients were capable of lysing fresh autologous tumor during a 4-hr 51Cr release assay. Multiple metastases from the same patient were equivalently lysed by these activated autologous PBL. No lysis of fresh PBL or lectin-induced lymphoblast cell targets was seen, although tumor, PBL, and lymphoblast cells were shown to be equally lysable using allosensitized cells. The activated cells could be expanded without loss of cytotoxicity in crude or lectin-free T-cell growth factors. The generation of cells lytic to fresh autologous tumor was dependent on the presence of adherent cells, although the lytic cell itself was not adherent. Proliferation was not involved in the induction of lytic cells since equal lysis was induced in irradiated and nonirradiated lymphocytes. Lectin was not required in the lytic assay, and the addition of alpha-methyl-D-mannoside to concanavalin A-activated lymphoid cells did not increase the lysis of fresh tumor cells. Activation by lectin for 3 days appears to be an efficient and convenient method for generating human cells lytic to fresh autologous tumor. These lytic cells may be of value for studies of the cell-mediated lysis of human tumor and possibly for tumor immunotherapy as well

  5. Harvest of autologous clavipectoral fascia for use in duraplasty: cadaveric feasibility study.

    Science.gov (United States)

    Louis, Robert G; Tubbs, R Shane; Mortazavi, Martin M; Shoja, Mohammadali M; Loukas, Marios; Cohen-Gadol, Aaron A

    2013-03-01

    Techniques and materials for repair of dural defects following neurosurgical procedures vary. Given higher complication rates with nonautologous duraplasty materials, most authors strongly recommend autologous grafts. To expand the arsenal of possible materials available to the neurosurgeon, we propose the use of autologous clavipectoral fascia as an alternative donor for duraplasty. Eight embalmed adult cadavers underwent dissection of the pectoral region. A 12-cm curvilinear skin incision was made 2 cm inferior to the nipple in males and along the inferior breast edge in females. Dissection was continued until the clavipectoral fascia was encountered, and a tissue plane was developed between this fascia and the deeper pectoralis major muscle. Sections of clavipectoral fascia were used for duraplasty in the same specimens. In all specimens, removal of clavipectoral fascia was easily performed with tissue separation between the overlying fascia and underlying muscle. Only small adhesions were found between the fascia and underlying muscle, and these were easily transected. No obvious gross neurovascular injuries were identified. Large portions of clavipectoral fascia were available, and at least a 10 × 10-cm piece (average thickness, 1.2 mm) was easily harvested for all specimens. Clavipectoral fascia shares characteristics with materials such as pericranium and fascia lata that have been used successfully in duraplasty, and most importantly, it is autologous. Theoretically, using clavipectoral fascia would reduce the risk of muscle herniation. It offers an alternative source for autologous dural grafting when other sources are unavailable or exhausted. Clinical experience with this fascia is warranted.

  6. Salmonella sepsis following posttraumatic splenectomy and implantation of autologous splenic tissue

    DEFF Research Database (Denmark)

    Schrøder, H M; Hovendal, C

    1985-01-01

    A severe complication following implantation of autologous splenic tissue occurred in a 51-year-old man. Indirect injury to abdomen resulted in a lesion of the splenic artery. Following splenectomy and reimplantation of splenic tissue into three pouches, a severe Salmonella sepsis developed within...

  7. Biodegradable p(DLLA-epsilon-CL) nerve guides versus autologous nerve grafts : Electromyographic and video analysis

    NARCIS (Netherlands)

    Meek, MF; Nicolai, JPA; Gramsbergen, A; van der Werf, J.F.A.

    The aim of this study was to evaluate the functional effects of bridging a gap in the sciatic nerve of the rat with either a biodegradable copolymer of (DL)-lactide and epsilon -caprolactone [p(DLLA-epsilon -CL)] nerve guide or an autologous nerve graft. Electromyograms (EMGs) of the gastrocnemius

  8. Poly(DL-lactide-epsilon-caprolactone) nerve guides perform better than autologous nerve grafts

    NARCIS (Netherlands)

    DenDunnen, WFA; VanderLei, B; Schakenraad, JM; Stokroos, [No Value; Blaauw, E; Pennings, AJ; Robinson, PH; Bartels, H.

    1996-01-01

    The aim of this study was to compare the speed and quality of nerve regeneration after reconstruction using a biodegradable nerve guide or an autologous nerve graft. We evaluated nerve regeneration using light microscopy, transmission electron microscopy and morphometric analysis. Nerve regeneration

  9. Physiological problems in patients undergoing autologous and allogeneic hematopoietic stem cell transplantation

    Directory of Open Access Journals (Sweden)

    Sevgisun Kapucu

    2014-01-01

    Full Text Available Objective: Stem cell transplantation is usually performed in an effort to extend the patient′s life span and to improve their quality of life. This study was conducted to determine the postoperative physiological effects experienced by patients who had undergone autologous and allogeneic stem cell transplantation. Methods: The research is a descriptive study conducted with a sample of 60 patients at Stem Cell Transplantation Units in Ankara. Percentile calculation and chi-square tests were used to evaluate the data. Results: When a comparison was made between patients who had undergone allogeneic Hematopoietic stem cell transplantation (HSCT and those who had undergone autologous HSCT, results indicated that problems occurred more often for the allogeneic HSCT patients. The problems included: Digestion (94.3%, dermatological (76.7%, cardiac and respiratory (66.7%, neurological (66.7%, eye (56.7%, infections (26.7% and Graft Versus Host Disease (5 patients. Furthermore, the problems with pain (50%, numbness and tingling (40%, and speech disorders (3 patients were observed more often in autologous BMT patients. Conclusion: Autologous and allogeneic patients experienced most of physical problems due to they receive high doses of chemotherapy. Therefore, it is recommended that an interdisciplinary support team approach should be usedtohelp reduce and manage the problems that may arise during patient care.

  10. Plan de empresa para la creación de un restaurante de comida casual argentina Punta Zur

    OpenAIRE

    Arango B., Gustavo Adolfo; Isaacs, Laura

    2011-01-01

    RESUMEN: Punta Zur, será un restaurante de comida casual dentro de la categoría de comida rápida, ubicado en Granada, Cali, que iniciará operaciones en Mayo de 2012. Los productos ofrecidos serán platos típicos del sur del continente americano, entre ellos el choripán y las brochetas (res, cerdo y pollo). También se ofrecerán acompañantes tales como papas, yucas fritas, chips de plátano y bebidas como jugos naturales, gaseosas y cervezas. En Punta Zur los clientes encontrarán un sitio en el q...

  11. Substitutes of structural and non-structural autologous bone grafts in hindfoot arthrodeses and osteotomies: a systematic review.

    Science.gov (United States)

    Müller, Marc Andreas; Frank, Alexander; Briel, Matthias; Valderrabano, Victor; Vavken, Patrick; Entezari, Vahid; Mehrkens, Arne

    2013-02-07

    Structural and non-structural substitutes of autologous bone grafts are frequently used in hindfoot arthrodeses and osteotomies. However, their efficacy is unclear.The primary goal of this systematic review was to compare autologous bone grafts with structural and non-structural substitutes regarding the odds of union in hindfoot arthrodeses and osteotomies. The Medline and EMBASE and Cochrane databases were searched for relevant randomized and non-randomized prospective studies as well as retrospective comparative chart reviews. 10 studies which comprised 928 hindfoot arthrodeses and osteotomies met the inclusion criteria for this systematic review. The quality of the retrieved studies was low due to small samples sizes and confounding variables. The pooled random effect odds for union were 12.8 (95% CI 12.7 to 12.9) for structural allografts, 5.7 (95% CI 5.5 to 6.0) for cortical autologous grafts, 7.3 (95% CI 6.0 to 8.6) for cancellous allografts and 6.0 (95% CI 5.7 to 6.4) for cancellous autologous grafts. In individual studies, the odds of union in hindfoot arthrodeses achieved with cancellous autologous grafts was similar to those achieved with demineralised bone matrix or platelet derived growth factor augmented ceramic granules. Our results suggest an equivalent incorporation of structural allografts as compared to autologous grafts in hindfoot arthrodeses and osteotomies. There is a need for prospective randomized trials to further clarify the role of substitutes of autologous bone grafts in hindfoot surgery.

  12. Autologous whole blood versus corticosteroid local injection in treatment of plantar fasciitis: A randomized, controlled multicenter clinical trial.

    Science.gov (United States)

    Karimzadeh, Afshin; Raeissadat, Seyed Ahmad; Erfani Fam, Saleh; Sedighipour, Leyla; Babaei-Ghazani, Arash

    2017-03-01

    Plantar fasciitis is the most common cause of heel pain. Local injection modalities are among treatment options in patients with resistant pain. The aim of the present study was to evaluate the effect of local autologous whole blood compared with corticosteroid local injection in treatment of plantar fasciitis. In this randomized controlled multicenter study, 36 patients with chronic plantar fasciitis were recruited. Patients were allocated randomly into three treatment groups: local autologous blood, local corticosteroid injection, and control groups receiving no injection. Patients were assessed with visual analog scale (VAS), pressure pain threshold (PPT), and plantar fasciitis pain/disability scale (PFPS) before treatment, as well as 4 and 12 weeks post therapy. Variables of pain and function improved significantly in both corticosteroid and autologous blood groups compared to control group. At 4 weeks following treatment, patients in corticosteroid group had significantly lower levels of pain than patients in autologous blood and control groups (higher PPT level, lower PFPS, and VAS). After 12 weeks of treatment, both corticosteroid and autologous blood groups had lower average levels of pain than control group. The corticosteroid group showed an early sharp and then more gradual improvement in pain scores, but autologous blood group had a steady gradual drop in pain. Autologous whole blood and corticosteroid local injection can both be considered as effective methods in the treatment of chronic plantar fasciitis. These treatments decrease pain and significantly improve function compared to no treatment.

  13. Ansätze zur Anreicherung der Lerngelegenheit Unterrichtspraktikum. Ausgewählte Modelle und deren empirische Erprobung

    OpenAIRE

    Kreis, Annelies

    2012-01-01

    Traditionelle Modelle zur Anregung des Lernens zukünftiger Lehrpersonen im Praktikum fokussieren die Funktionen von Mentorinnen und Mentoren vor allem auf die Beobachtung von Unterricht und dessen Nachbesprechung. In den letzten Jahren wurden zunehmend Ansätze eingeführt, die ein erweitertes Repertoire lernförderlicher Kooperation im Praktikum vorschlagen. Modelle wie das reflexive Praktikum, das fachspezifisch-pädagogische Coaching oder das Educative Mentoring empfehlen auf einer sozialkonst...

  14. Programmierung eines GKS-Plugins in Python zur Erzeugung von statischen JavaScript/HTML5 Inhalten

    OpenAIRE

    Knodt, David

    2013-01-01

    Die Wissenschaftler des Peter Grünberg Instituts/Jülich Centre for Neutron Science untersuchen in Experimenten und Simulationen Form und Dynamik von Materialien wie Polymeren, Zusammenlagerungen großer Moleküle und biologischer Zellen, sowie die elektronischen Eigenschaften von Festkörpern. Zur Visualisierung der Messdaten wird häufig ein Grafisches Kernsystem verwendet, welches eine einheitliche Schnittstelle und viele Ausgabemöglichkeiten bietet (z. B. PDF, Qt, PNG).Im Rahmen dieser Bachelo...

  15. Untersuchungen zur Ubiquitinylierung des PTS1-Rezeptors Pex5p im Rahmen des peroxisomalen Matrixproteinimports in der Hefe Saccharomyces cerevisiae

    OpenAIRE

    Denter, Christoph

    2010-01-01

    Die Translokation von Proteinen aus dem Zytosol in die peroxisomale Matrix erfolgt größtenteils Pex5p-abhängig. Pex5p wird im Rahmen des Rezeptorzyklusses an der peroxisomalen Membran durch die Anbindung von Ubiquitin unterschiedlich modifiziert, was zum einen die Degradation falsch gefalteter Proteine und zum anderen die Wiederverwertung des PTS1- Rezeptors zur Folge hat. Hier wurde anhand des Modellsystems der Bäckerhefe Saccharomyces cerevisiae die Ubiquitinylierung des Pex5p n...

  16. Delphy study `98: Experts forecast the future; Delphi-Studie 98: 2000 Experten aeussern sich zur Zukunft

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    1998-05-01

    In ancient Greece, a single priestess was sufficient to predict the future. In contrast, the `98 Delphy study comprises forecasts made by more than 2000 experts from the fields of research and economy. (orig./GL) [Deutsch] Waehrend vor ca. 2 500 Jahren im alten Griechenland eine einzige Priesterin ausreichte, die Zukunft vorherzusagen, sind fuer die Delphi-Studie 98 zur Zukunft Deutschlands die Prognosen von mehr als 2 000 Fachleuten aus Forschung und Wirtschaft zusammengefasst. (orig./GL)

  17. Practical guide to more efficient use of energy in industry; Praxisleitfaden zur Foerderung der rationellen Energieverwendung in der Industrie

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-09-04

    Starting with an easily digestible explanation of fundamental concepts and terms in the field of energy and its applications, as well as the related thermodynamic principles and processes, the second chapter explains the system of energy and exergy. Chapters 3 and 4 give a comprehensive picture of current technologies and processes in energy generation and engineering, from energy applications for process heat generation through to power plants and combined and heat and power systems, fuel cells and energy generation from renewable primary sources, discussing related aspects of interest such as energy and pollutant emissions, and economic aspects. Chapter 5 is devoted to counselling and guidance for corporate energy engineering analysis and enhancement. Chapters 6 and 7 round up the comprehensive information addressing the legal aspects of procedures for licensing of energy generation and conversion systems, and the various financing instruments and schemes available for projects intended to promote more efficient use of energy. (orig./CB) [Deutsch] Ausgehend von einer anschaulichen Beschreibung grundlegender Energiebegriffe und thermodynamischer Zusammenhaenge wird im zweiten Kapitel in klarer Weise das Zusammenspiel zwischen Energie und Exergie dargestellt. Aufbauend auf diesen grundlegenden Hinweisen bieten die Kapitel 3 und 4 eine weitreichende Uebersicht der gegenwaertig zur Verfuegung stehenden Prozess- und Energietechniken. Ausgehend von Techniken zur Prozesswaermebereitstellung spannt sich der Bogen ueber Kraftanlagen und Kraft-Waerme-Kopplungssysteme bis hin zu Brennstoffzellen und regenerativen Energietechniken, wobei der Schwerpunkt der Betrachtungen auf Energie- und Emissionsaspekte sowie wirtschaftliche Randbedingungen gelegt wurde. Kapitel 5 gibt Hinweise zur Vorbereitung und Durchfuehrung energietechnischer Beratungen und Analysen auf betrieblicher Ebene. Im Anschluss an den technischen Teil dieses Leitfadens folgt in Kapitel 6 eine Erlaeuterung der

  18. Furane, Furanone und Pyranone – ihr Beitrag zur Farbe und den antioxidativen Eigenschaften in der Maillard-Reaktion der Maltose

    OpenAIRE

    Kanzler, Clemens

    2017-01-01

    Die Maillard-Reaktion ist dafür bekannt, bei der thermischen Behandlung von Lebensmitteln neben deren organoleptischen Eigenschaften, wie Geschmack, Geruch, Textur und Farbe, auch die oxidative Stabilität zu beeinflussen. Letzteres ist auf die Bildung von komplexierenden und reduzierenden Verbindungen zurückzuführen, die antioxidativ und prooxidativ wirken können. Im Rahmen der vorliegenden Arbeit werden die antioxidativen Kapazitäten von dreizehn Maillard-Reaktionsintermediaten mit Furan-...

  19. Energy management. Measures on consumption-, and cost reduction, financing programs, regulations; Energiemanagement. Massnahmen zur Verbrauchs- und Kostenreduzierung, Foerderprogramme, Vorschriften

    Energy Technology Data Exchange (ETDEWEB)

    Hessel, Volker

    2008-07-01

    The book informs quickly and soundly on possibilities to reduce energy cost by load management and optimization of facilities, on fundamentals of energy management and valuable frame conditions, on relevant laws and important financing programs. (orig./GL) [German] Das Buch informiert schnell und fundiert ueber die Moeglichkeiten zur Reduzierung der Energiekosten durch Lastmanagement und Anlagenoptimierung, ueber die Grundlagen des Energiemanagements und gueltige Rahmenbedingungen, ueber relevante Gesetze und wichtige Foerderprogramme. (orig.)

  20. Untersuchungen zur Involution der Vagina and Zervix beim Rind post partum unter Berücksichtigung der Zervix als Geburtshindernis

    OpenAIRE

    Wehrend, Axel

    2002-01-01

    Ziel der vorliegenden Arbeit war es die physiologische Involution der Zervix im Vergleich zur Vagina beim Rind während der ersten zehn Tage post partum zu charakterisieren. Weiterhin sollte durch klinische Untersuchungen an Kühen nach Dystokie und einer retrospektiven Datenauswertung von Schwergeburten unterschiedlicher Tierarten der Anteil zervikal bedingter Geburtsstockungen im Interspeziesvergleich analysiert und Einflussfaktoren auf das Auftreten dieser Dystokieform sowie Störungen in der...

  1. Autologous fat transplantation: volumetric tools for estimation of volume survival. A systematic review.

    Science.gov (United States)

    Herold, Christian; Ueberreiter, Klaus; Busche, Marc N; Vogt, Peter M

    2013-04-01

    Autologous fat transplantation has gained great recognition in aesthetic and reconstructive surgery. Two main aspects are of predominant importance for progress control after autologous fat transplantation to the breast: quantitative information about the rate of fat survival in terms of effective volume persistence and qualitative information about the breast tissue to exclude potential complications of autologous fat transplantation. There are several tools available for use in evaluating the rate of volume survival. They are extensively compared in this review. The anthropometric method, thermoplastic casts, and Archimedes' principle of water displacement are not up to date anymore because of major drawbacks, first and foremost being reduced reproducibility and exactness. They have been replaced by more exact and reproducible tools such as MRI volumetry or 3D body surface scans. For qualitative and quantitative progress control, MRI volumetry offers all the necessary information: evaluation of fat survival and diagnostically valuable imaging to exclude possible complications of autologous fat transplantation. For frequent follow-up, e.g., monthly volume analysis, repeated MRI exams would not be good for the patient and are not cost effective. In these cases, 3D surface imaging is a good tool and especially helpful in a private practice setting where fast data acquisition is needed. This tool also offers the possibility of simulating the results of autologous fat transplantation. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

  2. Das Leben, der Tod und die staubige Wiedergeburt - Zur Vermittlung von Bo(otschaften zwischen Kunst und Wissenschaft

    Directory of Open Access Journals (Sweden)

    Slavko Kacunko

    2014-06-01

    Full Text Available In ihrer 'Bakterienkunst' greift Sabine Kacunko unter anderem auf die Patina und den Biofilm als Zeugnisse von Oberflächenveränderungen zurück, welche sie mithilfe von naturwissenschaftlichen und medialen Techniken auch für die Kunst erschließbar macht. Der folgende Beitrag macht sich zur Aufgabe, die interdisziplinäre und medienpädagogischen Potenziale des entsprechenden, bislang ungenügend kontextualisierten, Forschungsfeldes vorzustellen. Am deutlichsten vermittelbar war das Interesse an Bakterien bislang vor allem durch die bezügliche forschungsbasierte Kunst gewesen. Diese soll hier an einer künstlerischen Position verdeutlicht werden, welche die Breite des genannten Forschungsfeldes bewusst zum eigenen, fortlaufenden Programm gemacht hat. Die derzeit global verbreiteten akademischen Programme zur kunst-basierten Forschung (Art-based Research; Practice-based Research etc. untersuchen bekanntlich die Querverbindungen zwischen Forschung durch Kunst, Forschung über die Kunst und Forschung, welche die Kunst benützt. Definiert wird diese disziplinübergreifende kunst-basierte Forschung einerseits durch den systematischen Gebrauch von künstlerischen Prozessen als Quellen zum Verstehen und Vermittlung von Erfahrungen von Forschern, Institutionen und Publikum. Sie liefert andererseits Einsichten in die übergreifenden epistemischen Wendungen (twists, welche wiederum eine hohe Relevanz für die künftige Bildung und professionelle Praxis darstellen.

  3. An algorithm for calculating unsteady flow with free surface; Ein Verfahren zur Berechnung instationaerer Stroemungen mit freier Oberflaeche

    Energy Technology Data Exchange (ETDEWEB)

    Janetzky, B.

    2001-07-01

    A numerical model for the transient, free surface flow is implemented in a Finite-Element program for the unsteady calculation of incompressible flow with free surface. The program is used to calculate the flow in different components of a hydraulic turbine, the Pelton turbine. The movement of the fluid with free surface is described mathematically by introducing a partial differential equation for the volume fraction. This equation is simply a transport equation for f, i.e. the volume fraction is advected with the flow in time. The equations is solved numerically. (orig.) [German] Es wird ein Verfahren zur Modellierung von veraenderlichen, freien Oberflaechen vorgestellt und in einem Finite-Elemente-Programm zur numerischen Berechnung von instationaeren, inkompressiblen Stroemungen implementiert. Die veraenderliche, freie Oberflaeche wird mit einem Volume-Of-Fluid Ansatz erfasst. Zur Approximierung der freien Oberflaeche werden stueckweise konstante oder gestufte Verlaeufe im Element angesetzt. Es werden die Eigenschaften des Verfahrens an ausgewaehlten Beispielen mit freier Oberflaeche untersucht. Das erweiterte Programm wird auf instationaere Stroemungen mit freier Oberflaeche in einer hydraulischen Maschine, der Peltonturbine, angewandt. (orig.)

  4. Paediatric Virology as a new educational initiative: An interview with Nobelist Professor of Virology Harald zur Hausen.

    Science.gov (United States)

    Mammas, Ioannis N; Spandidos, Demetrios A

    2017-10-01

    Born in Gelsenkirchen-Buer in Germany on March 11th, 1936, Professor Harald zur Hausen, Emeritus Professor of Virology at the University of Freiburg and 2008 Nobel Prize Laureate in Physiology or Medicine for his discovery of human papillomavirus (HPV), which causes cervical cancer, believes that good knowledge of virological methods and diagnostic possibilities are an asset for all young paediatricians. Professor zur Hausen considers that the creation of an educational platform on Paediatric Virology is definitely very beneficial for young paediatricians, as this will greatly enhance their knowledge in the field of Virology. He very actively advocates the vaccination of boys for the eradication of HPV infection and emphasises that male HPV vaccination should be included into the current vaccination programmes. He would have certainly considered Dr George N. Papanicolaou (Kyme, Island of Euboea, Greece, 1883 - Miami, Florida, USA, 1962) as an excellent candidate for the Nobel Prize, stating that the contribution of Dr Papanicolaou did not find sufficient recognition in the past. In the context of the 3rd Workshop on Paediatric Virology, which will be held in Athens, Greece, on October 7th, 2017, Professor zur Hausen will give his plenary lecture on 'Paediatric Virology and Oncology: Virus persistence and the important first years of life'.

  5. The current status of autologous blood transfusion in Japan--the importance of pre-deposit autologous blood donation program and the needs to achieve patient blood management.

    Science.gov (United States)

    Tsuno, Nelson Hirokazu; Nagura, Yutaka; Kawabata, Michiru; Matsuhashi, Mika; Sone, Shinji; Ikeda, Toshiyuki; Okochi, Naoko; Takahashi, Koki

    2013-12-01

    Autologous blood transfusion (ABT) is currently considered the safest transfusion, since the risks of allogeneic immunological reaction and viral transmission are theoretically null. Although its use has declined in Western countries in the recent decade, it has been progressively expanded in Japan. With the widening of the concept of patient blood management (PBM), which aims to prevent the harmful adverse effects of the exposure to allogeneic blood, the importance of the ABT has once again gained interest. Here, we retrospectively analyzed the cases pre-depositing autologous blood for an elective surgery in the period of January 2000 to December 2010 in our hospital, where a pre-deposit autologous blood donation (PAD) program has been established in 2006, in an attempt to analyze the improvements achieved, and the problems remaining to achieve patient blood management. The PAD program contributed for the further improvement of ABT, and the number of participating patients increased, especially in the period 2002-2003, when the idea of PAD program implementation came out. By simple extrapolation of the ABT data to allogeneic blood, ABT was found to be superior in terms of cost-effectiveness. However, problems such as the high wastage rate, and the inappropriate transfusion triggers remain to be solved. ABT plays the central role in PBM, but to achieve the real PBM, there is need to indicate ABT appropriately, according to the individual needs, and use it adequately, without discarding. Our present data reflect the present status of the ABT performance in Japan, and will serve as the basis for the development of strategies to achieve safe and appropriate performance of ABT, and consequently, achieve PBM. Copyright © 2013 Elsevier Ltd. All rights reserved.

  6. Usefulness of concomitant autologous blood and dextranomer/hyaluronic acid copolymer injection to correct vesicoureteral reflux.

    Science.gov (United States)

    Kajbafzadeh, Abdol-Mohammad; Tourchi, Ali

    2012-09-01

    We present the long-term results of a new modification of endoscopic treatment of vesicoureteral reflux involving concomitant injection of autologous blood following the standard hydrodistention injection technique to prevent bulking agent leakage immediately after the procedure. A total of 341 children underwent endoscopic implantation of dextranomer/hyaluronic acid for vesicoureteral reflux. A subset of 171 patients underwent hydrodistention autologous blood injection, while 170 underwent classic hydrodistention injection. Frequency of symptomatic urinary tract infection after endoscopic treatment was recorded. Success was defined as absence of vesicoureteral reflux on postoperative voiding cystourethrography. A total of 523 ureters in 214 girls and 127 boys were treated. In patients undergoing hydrodistention autologous blood injection mean age was 39.48 months, mean maximal reflux grade was 3.02 and success rate was 93.6% after the first injection (98.0% in patients with grade II, 92.1% with grade III, 93.3% with grade IV and 85.7% with grade V reflux). In patients who underwent classic hydrodistention injection mean age was 36.12 months, mean maximal reflux grade 3.05 and success rate was 81.8% after the first injection (91.5% in patients with grade II, 89.4% with grade III, 74.4% with grade IV and 44.4% with grade V reflux). The success rate was significantly higher (p = 0.001) in patients undergoing hydrodistention autologous blood injection vs classic hydrodistention injection. Of the patients 1.7% in the hydrodistention autologous blood injection group and 2.9% in the classic hydrodistention injection group reported symptomatic urinary tract infection during followup. Immediate injection of autologous blood following dextranomer/hyaluronic acid injection to create a blood clot and barricade against bulking agent leakage is more effective than pure dextranomer/hyaluronic acid implantation. This novel modification stabilizes the subureteral implant mount and

  7. Autologous bone marrow mononuclear cell transplant and surgical decompression in a dog with chronic spinal cord injury.

    Science.gov (United States)

    Tamura, Katsutoshi; Harada, Yasuji; Kunimi, Maki; Takemitsu, Hiroshi; Hara, Yasushi; Nakamura, Tatsuo; Tagawa, Masahiro

    2015-02-01

    In dogs with deep analgesia caused by acute spinal cord injury from thoracolumbar disk herniation, autologous bone marrow mononuclear cell transplant may improve recovery. The purpose of the present study was to evaluate autologous bone marrow mononuclear cell transplant in a dog that had paraplegia and deep analgesia caused by chronic spinal cord injury. Autologous bone marrow mononuclear cell transplant was performed in a dog having paraplegia and analgesia for 3 years that was caused by a chronic spinal cord injury secondary to Hansen type I thoracolumbar disk herniation. Functional recovery was evaluated with electrophysiologic studies and the Texas Spinal Cord Injury Scale. Somatosensory evoked potentials were absent before transplant but were detected after transplant. Functional improvement was noted (Texas Spinal Cord Injury Scale: before transplant, 0; after transplant, 6). No adverse events were observed. Autologous bone marrow mononuclear cell transplant into the subarachnoid space may be a safe and beneficial treatment for chronic spinal cord injury in dogs.

  8. Imaging collagen remodeling and sensing transplanted autologous fibroblast metabolism in mouse dermis using multimode nonlinear optical imaging

    International Nuclear Information System (INIS)

    Zhuo Shuangmu; Chen Jianxin; Jiang Xingshan; Xie Shusen; Cao Ning; Xiong Shuyuan

    2008-01-01

    Collagen remodeling and transplanted autologous fibroblast metabolic states in mouse dermis after cellular injection are investigated using multimode nonlinear optical imaging. Our findings show that the technique can image the progress of collagen remodeling in mouse dermis. It can also image transplanted autologous fibroblasts in their collagen matrix environment in the dermis, because of metabolic activity. It was also found that the approach can provide two-photon ratiometric redox fluorometry based on autologous fibroblast fluorescence from reduced nicotinamide adenine dinucleotide coenzyme and oxidized flavoproteins for sensing the autologous fibroblast metabolic state. These results show that the multimode nonlinear optical imaging technique may have potential in a clinical setting as an in vivo diagnostic and monitoring system for cellular therapy in plastic surgery

  9. MR cartilage imaging in assessment of the regenerative power of autologous peripheral blood stem cell injection in knee osteoarthritis

    Directory of Open Access Journals (Sweden)

    Khaled A. Ahmad

    2014-09-01

    Conclusion: Limited good level of evidence showed that repeated intra-articular injections of autologous PBSC resulted in an improvement of the quality of articular cartilage repair and physical function as observed by MRI and clinical assessment.

  10. Bone regeneration using the freshly isolated autologous stromal vascular fraction of adipose tissue in combination with calcium phosphate ceramics

    NARCIS (Netherlands)

    Prins, H.J.; Schulten, E.A.J.M.; ten Bruggenkate, C.M.; Klein-Nulend, J.; Helder, M.N.

    2016-01-01

    In patients undergoing maxillary sinus floor elevation (MSFE) for dental implant placement, bone substitutes are currently evaluated as alternatives for autologous bone. However, bone substitutes have only osteoconductive properties and lack osteoinductive potential. Therefore, this phase I study

  11. Hybrid plants as a means of power supply; Hybridanlagen zur Energieversorgung

    Energy Technology Data Exchange (ETDEWEB)

    Cramer, G. [SMA System-, Mess- und Anlagentechnik - Regelsysteme GmbH, Niestetal (Germany)

    1997-02-01

    SMA Regelsysteme GmbH has developed a modular system for the power output range from 30 kW to 5 MW as a means of power supply for remote locations and settlements that are not connected to the public grid. Around the globe more than 30 of these systems have demonstrated their availability and cost effectiveness under sometimes extreme operating conditions. The Intelligent Power System is currently the only series-produced, commercially available wind/diesel/battery system worldwide capable of supplying remote regions with electrical energy. The Intelligent Power System consists of one or several diesel generators, a battery storage with power converter, a rotating phase shifter and in some cases one or several aerogenerators. In some applications there is also an integrated photovoltaic generator. The results obtained with systems of this kind around the globe show that despite the high initial investment costs power production costs are up to 50% lower than in the case of conventional diesel-fueled power plants. This is achieved by savings on diesel fuel, short overall operating times of the diesel aggregates and drastically reduced labour costs. The paper also addresses the experiences gained with battery storages and the R and D requirements for optimising the operation of battery storages. SMA is currently cooperating with ISET in developing a new systems approach for very small power supply systems which will be distinguished by its modular expandability, amongst other features. [German] Zur elektrischen Energieversorgung entlegener Ortschaften und Ansiedlungen, die nicht an ein oeffentliches Verbundnetz angeschlossen sind, hat SMA Regelsysteme GmbH ein modulares System fuer den Leistungsbereich von 30 kW bis 5 MW entwickelt. Mehr als 30 dieser Systeme haben ihre Verfuegbarkeit und Wirtschaftlichkeit unter z.T. extremen Einsatzbedingungen weltweit unter Beweis gestellt. Das Intelligent Power System (IPS) ist das z.Zt. einzige serienproduzierte und kommerziell

  12. Intra-arterial Autologous Bone Marrow Cell Transplantation in a Patient with Upper-extremity Critical Limb Ischemia

    Energy Technology Data Exchange (ETDEWEB)

    Madaric, Juraj, E-mail: jurmad@hotmail.com [National Institute of Cardiovascular Diseases (NUSCH) and Slovak Medical University, Department of Cardiology and Angiology (Slovakia); Klepanec, Andrej [National Institute of Cardiovascular Diseases, Department of Diagnostic and Interventional Radiology (Slovakia); Mistrik, Martin [Clinic of Hematology and Transfusiology, Faculty Hospital (Slovakia); Altaner, Cestmir [Slovak Academy of Science, Institute of Experimental Oncology (Slovakia); Vulev, Ivan [National Institute of Cardiovascular Diseases, Department of Diagnostic and Interventional Radiology (Slovakia)

    2013-04-15

    Induction of therapeutic angiogenesis by autologous bone marrow mononuclear cell transplantation has been identified as a potential new option in patients with advanced lower-limb ischemia. There is little evidence of the benefit of intra-arterial cell application in upper-limb critical ischemia. We describe a patient with upper-extremity critical limb ischemia with digital gangrene resulting from hypothenar hammer syndrome successfully treated by intra-arterial autologous bone marrow mononuclear cell transplantation.

  13. [Platelet-rich plasma combined with autologous cancellous bone : An alternative therapy for persistent non-union?].

    Science.gov (United States)

    Hakimi, M; Jungbluth, P; Thelen, S; Betsch, M; Linhart, W; Flohé, S; Windolf, J; Wild, M

    2011-11-01

    In addition to a stabile osteosynthesis autologous cancellous bone graft remains an essential therapy option in persistent non-union. Despite this therapy regimen persistent non-union can occasionally occur. The aim of this study was to evaluate the treatment of persistent non-union with a combination of platelet-rich plasma (PRP) and autologous cancellous bone. In this prospective study 17 patients with persistent non-union of long bones were treated by a combination of PRP and autologous iliac crest bone. Inclusion criteria were a minimum of one previously failed cancellous bone transplantation and an atrophic non-union persisting for 6-14 months (mean 9 months). The patients were examined clinically and radiologically at intervals of 3, 6 and 9 months postoperatively. After an average time of 17 months (range 15-23 months) the patients were treated by a combination of PRP and autologous cancellous bone. In all cases the non-union was successfully treated and osseous bridging was found radiologically after an average of 5 months (range 4-7 months) without any complications. The combination of PRP and autologous cancellous bone appears to be a safe and effective method for treatment of persistent non-union. The use of PRP does not result in substantial additional costs. Allergies and graft versus host reactions are not expected because of the autologous origin.

  14. Comparison of the osteogenic potentials of autologous cultured osteoblasts and mesenchymal stem cells loaded onto allogeneic cancellous bone granules.

    Science.gov (United States)

    Kim, Seok-Jung; Chung, Yang-Guk; Lee, Yun-Kyoung; Oh, Il-Whan; Kim, Yong-Sik; Moon, Young-Seok

    2012-02-01

    We compared the bone regeneration potentials of autologous cultured osteoblasts and of bone-marrow-derived autologous MSCs in combination with allogeneic cancellous bone granules in a rabbit radial defect model. Radial shaft defects over 15 mm were made in 26 New Zealand white rabbits. The animals underwent insertion of allogeneic cancellous bone granules containing autologous osteoblasts into right-side defects (the experimental group) and of allogeneic cancellous bone granules with autologous MSCs into left-side defects (the control group). To quantitatively assess bone regeneration, radiographic evaluations as well as BMD and BMC measurements were performed 3, 6, 9 and 12 weeks post-implantation and histology as well as micro-CT image analysis were performed at 6 and 12 weeks. Radiographic evaluations 3 weeks post-implantation showed that the experimental group had a higher mean bone quantity index (p bone volume and surface area than the control sides (p bone formation in the experimental group. This in vivo study demonstrates that a combination of autologous osteoblasts and small-sized, allogeneic cancellous bone granules leads to more rapid bone regeneration than autologous MSCs and small-sized, allogeneic cancellous bone granules.

  15. Stability of simultaneously placed dental implants with autologous bone grafts harvested from the iliac crest or intraoral jaw bone.

    Science.gov (United States)

    Kang, Young-Hoon; Kim, Hyun-Min; Byun, June-Ho; Kim, Uk-Kyu; Sung, Iel-Yong; Cho, Yeong-Cheol; Park, Bong-Wook

    2015-12-30

    Jaw bone and iliac bone are the most frequently used autologous bone sources for dental implant placement in patients with atrophic alveolar ridges. However, the comparative long-term stability of these two autologous bone grafts have not yet been investigated. The aim of this study was to compare the stability of simultaneously placed dental implants with autologous bone grafts harvested from either the iliac crest or the intraoral jaw bone for severely atrophic alveolar ridges. In total, 36 patients (21 men and 15 women) were selected and a retrospective medical record review was performed. We compared the residual increased bone height of the grafted bone, peri-implantitis incidence, radiological density in newly generated bones (HU values), and implant stability using resonance frequency analysis (ISQ values) between the two autologous bone graft groups. Both autologous bone graft groups (iliac bone and jaw bone) showed favorable clinical results, with similar long-term implant stability and overall implant survival rates. However, the grafted iliac bone exhibited more prompt vertical loss than the jaw bone, in particular, the largest vertical bone reduction was observed within 6 months after the bone graft. In contrast, the jaw bone graft group exhibited a slower vertical bone resorption rate and a lower incidence of peri-implantitis during long-term follow-up than the iliac bone graft group. These findings demonstrate that simultaneous dental implantation with the autologous intraoral jaw bone graft method may be reliable for the reconstruction of edentulous atrophic alveolar ridges.

  16. Histomorphometric Evaluation of Superovulation Effect on Follicular Development after Autologous Ovarian Transplantation in Mice

    Directory of Open Access Journals (Sweden)

    Amin Tamadon

    2015-01-01

    Full Text Available The effect of superovulation by pregnant mare serum gonadotropin (PMSG on autologous transplanted ovaries in the lumbar muscles of mice was histomorphometrically evaluated using the indices of number and volume of different kind of follicles and volume of corpora lutea, ovary, and stroma. Angiogenesis was observed after mouse ovarian transplantation on days 14 and 21 after ovarian grafting. After transplantation, the total number and volume of primary and secondary follicles reduced, while PMSG superovulation increased the total number and total volume of tertiary follicles and also the ovarian volume after transplantation. Transplantation increased the average size of primary, secondary, and tertiary follicles. Therefore, primary and secondary follicles can survive after autologous transplantation but their reservations diminished by increasing the time of transplantation. However, number of tertiary follicles and their response to superovulation increased over time after transplantation.

  17. Autologous bone marrow mononuclear cells transplant in patients with critical leg ischemia: preliminary clinical results.

    Science.gov (United States)

    Li, Min; Zhou, Hua; Jin, Xing; Wang, Mo; Zhang, Shiyi; Xu, Lei

    2013-10-01

    Stem cell transplant can induce vasculogenesis and improve the blood supply to an ischemic region, offering hope for chronic lower extremity ischemic diseases. Bone marrow mononuclear cells are one of the sources for stem cell transplants. We sought to observe the safety and efficacy of autologous bone marrow mononuclear cells transplant for treating critical limb ischemia. Eligible patients were randomized 1:1 to receive placebo (0.9% NaCl) or 1 × 107 piece/mL bone marrow mononuclear cell transplant. For 6 months, patients' skin ulcers, ankle-brachial index, and rest pain were examined and recorded before and after treatment. Six months after the bone marrow mononuclear cells transplant, clinical symptoms like rest pain and skin ulcers gradually abated (P transplant (P Autologous bone marrow mononuclear cells transplant for treatment of patients with chronic limb ischemia is safe, effective, and feasible.

  18. Autologous Fat Grafting in the Treatment of Painful Postsurgical Scar of the Oral Mucosa

    Directory of Open Access Journals (Sweden)

    Andrea Lisa

    2015-01-01

    Full Text Available Background. Persistent pain as a consequence of surgical treatment has been reported for several common surgical procedures and represents a clinical problem of great magnitude. Material and Methods. We describe the case of a 47-year-old female who presented a retractile scar that adhered to deep planes at the upper right of the vestibule due to surgical removal of maxillary exostosis, which determined important pain symptoms extending till the right shoulder during both chewing and rest. We subsequently treated her with autologous fat grafting according to Coleman’s technique. Results. Clinical assessments were performed at 5 and 14 days, 1, 3, and 6 months, and 1 year after surgical procedure. We observed a progressive release of scar retraction together with an important improvement of pain symptoms. Conclusion. The case described widens the possible application of autologous fat grafting on a new anatomical site as buccal vestibule and in one specific clinical setting confirming its promising biological effects.

  19. Improving oocyte quality by transfer of autologous mitochondria from fully grown oocytes

    DEFF Research Database (Denmark)

    Kristensen, Stine Gry; Pors, Susanne Elisabeth; Andersen, Claus Yding

    2017-01-01

    options using autologous mitochondria to potentially augment pregnancy potential in ART. Autologous transfer of mitochondria from the patient's own germline cells has attracted much attention as a possible new treatment to revitalize deficient oocytes. IVF births have been reported after transfer...... of oogonial precursor cell-derived mitochondria; however, the source and quality of the mitochondria are still unclear. In contrast, fully grown oocytes are loaded with mitochondria which have passed the genetic bottleneck and are likely to be of high quality. An increased supply of such oocytes could...... with high quality mitochondria can be obtained from natural or stimulated ovaries and potentially be used to improve both quality and quantity of oocytes available for fertility treatment....

  20. Autologous Platelet Concentrates as Treatment for Avascular Necrosis of Femoral Head in a Dog.

    Science.gov (United States)

    Parra, Estefanía; Vergara, Andrea; Silva, Raúl F

    2017-03-01

    Avascular necrosis of the femoral head is a developmental disturbance that generally affects young dogs of small breeds and produces ischemic necrosis of the femoral head resulting in an incongruous and malformed joint. The most common treatment is the excisional arthroplasty of the head and femoral neck. The aim of this study is to describe the treatment of avascular necrosis in a Yorkshire dog using intra-articular injections of autologous platelet concentrate. Evaluations were made at 0, 15, 30, 60, and 120 days of treatment, describing the following parameters: clinical gait analysis, perimetry, goniometry, and radiographic evaluations. The results obtained in this case suggest that the autologous platelet concentrate may be an alternative for the treatment of avascular necrosis of the femoral head in dogs. Copyright © 2017 Elsevier Inc. All rights reserved.

  1. UPDATE ON THE ROLE OF AUTOLOGOUS HEMATOPOIETIC STEM CELL TRANSPLANTATION IN MULTIPLE MYELOMA

    Directory of Open Access Journals (Sweden)

    Patrizia Tosi

    2012-11-01

    Full Text Available Autologous stem cell transplantation is considered the standard of care for multiple myeloma patients aged < 65 years with no relevant comorbidities. The addition of drugs acting both on bone marrow microenvironment and on neoplastic plasma cells has significantly increased the proportion of patients achieving a complete remission after induction therapy, and these results are mantained after high-dose melphalan, leading to a prolonged disease control. Studies are being carried out in order to evaluate whether short term consolidation or long-term maintenance therapy can result into disease eradication at the molecular level thus increasing also patients survival. The efficacy of these new drugs has raised the issue of deferring the transplant after achivng a second response upon relapse. Another controversial point is the optimal treatment strategy for high-risk patients, that do not benefit from autologous stem cell transplantation and for whom the efficacy of new drugs is still matter of debate.

  2. Regulatory challenges for autologous tissue engineered products on their way from bench to bedside in Europe.

    Science.gov (United States)

    Ram-Liebig, Gouya; Bednarz, Juergen; Stuerzebecher, Burkard; Fahlenkamp, Dirk; Barbagli, Guido; Romano, Giuseppe; Balsmeyer, Ulf; Spiegeler, Maria-Elsa; Liebig, Soeren; Knispel, Helmut

    2015-03-01

    Since the late eighties of last century the high potential of tissue engineered products (TEP)s has been shown for the treatment of various diseases and many scientific publications appeared in this field. However, only few products reached the market since. Development of TEPs is a promising but owing to its novelty a very challenging task that requires experts in this still developing field as well as ample financial resources. This paper summarises relevant regulatory challenges during quality, preclinical and clinical development of autologous TEPs in Europe. Selected strategies on how to manage major issues are presented, together with some examples from the development of an autologous TEP for urethroplasty. Considering these aspects may help other investigators with potential strategies during the development of novel TEPs. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Qualification of academic facilities for small-scale automated manufacture of autologous cell-based products.

    Science.gov (United States)

    Hourd, Paul; Chandra, Amit; Alvey, David; Ginty, Patrick; McCall, Mark; Ratcliffe, Elizabeth; Rayment, Erin; Williams, David J

    2014-01-01

    Academic centers, hospitals and small companies, as typical development settings for UK regenerative medicine assets, are significant contributors to the development of autologous cell-based therapies. Often lacking the appropriate funding, quality assurance heritage or specialist regulatory expertise, qualifying aseptic cell processing facilities for GMP compliance is a significant challenge. The qualification of a new Cell Therapy Manufacturing Facility with automated processing capability, the first of its kind in a UK academic setting, provides a unique demonstrator for the qualification of small-scale, automated facilities for GMP-compliant manufacture of autologous cell-based products in these settings. This paper shares our experiences in qualifying the Cell Therapy Manufacturing Facility, focusing on our approach to streamlining the qualification effort, the challenges, project delays and inefficiencies we encountered, and the subsequent lessons learned.

  4. Autologous human tissue-engineered heart valves: prospects for systemic application.

    Science.gov (United States)

    Mol, Anita; Rutten, Marcel C M; Driessen, Niels J B; Bouten, Carlijn V C; Zünd, Gregor; Baaijens, Frank P T; Hoerstrup, Simon P

    2006-07-04

    Tissue engineering represents a promising approach for the development of living heart valve replacements. In vivo animal studies of tissue-engineered autologous heart valves have focused on pulmonary valve replacements, leaving the challenge to tissue engineer heart valves suitable for systemic application using human cells. Tissue-engineered human heart valves were analyzed up to 4 weeks and conditioning using bioreactors was compared with static culturing. Tissue formation and mechanical properties increased with time and when using conditioning. Organization of the tissue, in terms of anisotropic properties, increased when conditioning was dynamic in nature. Exposure of the valves to physiological aortic valve flow demonstrated proper opening motion. Closure dynamics were suboptimal, most likely caused by the lower degree of anisotropy when compared with native aortic valve leaflets. This study presents autologous tissue-engineered heart valves based on human saphenous vein cells and a rapid degrading synthetic scaffold. Tissue properties and mechanical behavior might allow for use as living aortic valve replacements.

  5. Autologous stromal vascular fraction therapy for rheumatoid arthritis: rationale and clinical safety

    Directory of Open Access Journals (Sweden)

    Rodriguez Jorge

    2012-02-01

    Full Text Available Abstract Advancements in rheumatoid arthritis (RA treatment protocols and introduction of targeted biological therapies have markedly improved patient outcomes, despite this, up to 50% of patients still fail to achieve a significant clinical response. In veterinary medicine, stem cell therapy in the form of autologous stromal vascular fraction (SVF is an accepted therapeutic modality for degenerative conditions with 80% improvement and no serious treatment associated adverse events reported. Clinical translation of SVF therapy relies on confirmation of veterinary findings in targeted patient populations. Here we describe the rationale and preclinical data supporting the use of autologous SVF in treatment of RA, as well as provide 1, 3, 6, and 13 month safety outcomes in 13 RA patients treated with this approach.

  6. [Autologous stem cell transplantation for autoimmune diseases: recommendations from the SFGM-TC].

    Science.gov (United States)

    Farge, D; Terriou, L; Badoglio, M; Cras, A; Desreumaux, P; Hadj-Khelifa, S; Marjanovic, Z; Moisan, A; Dulery, R; Faucher, C; Hij, A; Martin, T; Vermersch, P; Yakoub-Agha, I

    2014-08-01

    Autologous hematopoietic stem cell transplantation is a valid alternative to immunosuppressive treatment in patients with auto-immune disease; however, the role of this approach remains subject to debate. In the attempt to harmonize clinical practices between different French transplantation centers, the French Society of Bone Marrow Transplantation and Cell Therapies (SFGM-TC) set up its fourth annual series of workshops which brought together practitioners from all of its member centers. These workshops took place in September 2013 in Lille. In this article we give an overview regarding the indications of autologous stem cell transplantation in auto-immune diseases as well as recommendations regarding post-transplant follow-up of patients. Copyright © 2014. Published by Elsevier SAS.

  7. Studien zur Messung von Rekonstruktionseffizienz und Untergrund der $\\tau$-Lepton-Identifikation im Zerfall $Z\\to \\tau \\tau$ beim ATLAS-Experiment aus Daten

    CERN Document Server

    Johnert, Sebastian

    2008-01-01

    In dieser Diplomarbeit werden zwei Methoden vorgestellt, mit denen τ -Leptonen in den zuk ̈nf- u tigen Daten des ATLAS-Experiments untersucht werden sollen. Den ersten Teil bildet die Be- stimmung von Missidentifikationsraten von Jets aus QCD-2-Jet-Ereignissen als τ -Leptonen. Der zweite Teil ist die Entwicklung einer Methode zur Bestimmung der τ -Rekonstruktions- und -Identifikationseffizienz relativ zur μ-Effizienz. In diesem Zusammenhang werden invariante Massen aus Z → ll-Ereignissen bestimmt, die Massen aus Z → τ τ -Ereignissen mit denen aus Z → ee und Z → μμ verglichen, τ -Effizienzen gemittelt uber alle Bereiche und in verschiedenen ̈ o η-Bereichen berechnet sowie eine M ̈glichkeit zur Bestimmung von τ -Effizienzen in unterschied- lichen Transversalimpulsbereichen vorgestellt. Des Weiteren wird eine verbesserte Absch ̈tzung a des QCD-Untergrunds vorgenommen und das Verhalten der τ -Effizienz unter Ber ̈cksichtigung u des Triggers untersucht.

  8. Balancing and dynamic behaviour of a biofilter plant for complete cleaning of municipal sewage; Bilanzierung und dynamisches Verhalten einer Biofilteranlage zur Vollreinigung von kommunalem Abwasser

    Energy Technology Data Exchange (ETDEWEB)

    Dickgreber, M.; Luebbecke, S. [Preussag Wassertechnik GmbH, Bremen (Germany)

    1999-07-01

    Where municipal sewage treatment plants need to be extended or enlarged, biofilter systems represent an economical alternative. They can be used both for supplementary residual elimination purposes and to treat complete sewage streams. For a biofilter system for complete cleaning of sewage employing the DENIPOR trademark -NCS process which has been in operation since 1996 (2*500 population value), studies in process dynamics are carried out and a complete balance is set up. (orig.) [German] Biofilter stellen bei notwendigen Erweiterungen und Ausbaumassnahmen von kommunalen Klaeranlagen eine wirtschaftliche Alternative dar. Sie koennen fuer weitergehende Restreinigungsaufgaben und zur Vollstrombehandlung eingesetzt werden. An einer seit 1996 in Betrieb befindlichen Biofilteranlage zur Vollreinigung von kommunalem Abwasser nach dem DENIPOR {sup trademark} -NCS Verfahren (2*500EW) werden Untersuchungen zur Prozessdynamik sowie eine vollstaendige Bilanzierung durchgefuehrt. (orig.)

  9. Exploring the use of expanded erythroid cells for autologous transfusion for anemia of prematurity.

    Science.gov (United States)

    Khodabux, Chantal M; van Hensbergen, Yvette; Slot, Manon C; Bakker-Verweij, Margreet; Giordano, Piero C; Brand, Anneke

    2013-12-01

    Autologous cord blood (CB) red blood cells (RBCs) can partly substitute transfusion needs in premature infants suffering from anemia. To explore whether expanded CB cells could provide additional autologous cells suitable for transfusion, we set up a simple one-step protocol to expand premature CB cells. CB buffy coat cells and isolated CD34-positive (CD34(pos) ) cells from premature and full-term CB and adult blood were tested with several combinations of growth factors while omitting xenogeneic proteins from the culture medium. Cell differentiation was analyzed serially during 21 days using flow cytometry, progenitor assays, and high-performance liquid chromatography. Expanded CB buffy coat cells resulted in a threefold higher number of erythroblasts than the isolated CD34(pos) cells. However, the RBCs contaminating the buffy coat remained present during the culture with uncertain quality. Premature and full-term CB CD34(pos) cells had similar fold expansion capacity and erythroid differentiation. With the use of interleukin-3, stem cell factor, and erythropoietin, the fold increases of all CD34(pos) cell sources were similar: CB 3942 ± 1554, adult peripheral mobilized blood 4702 ± 1826, and bone marrow (BM) 4143 ± 1908. The proportion of CD235a expression indicating erythroblast presence on Day 21 was slightly higher in the adult CD34(pos) cell sources: peripheral blood stem cells (96.7 ± 0.8%) and BM (98.9 ± 0.5%) compared to CB (87.7 ± 2.7%; p = 0.002). We were not able to induce further erythroid maturation in vitro. This explorative study showed that fairly pure autologous erythroid-expanded cell populations could be obtained by a simple culture method, which should be optimized. Future challenges comprise obtaining ex vivo enucleation of RBCs with the use of a minimal manipulating approach, which can add up to autologous RBCs derived from CB in the treatment of anemia of prematurity. © 2013 American Association of Blood Banks.

  10. Autologous stem-cell transplantation in Hodgkin’s lymphoma: analysis of a therapeutic option

    Directory of Open Access Journals (Sweden)

    Adriano de Moraes Arantes

    2011-06-01

    Full Text Available Objective: To report the clinical progress of patients with Hodgkin’slymphoma treated with autologous transplantation after failure orrelapse of first-line treatment with chemotherapy and/or radiationtherapy. Methods: The results of a retrospective analysis of 31patients submitted to autologous transplantation as second-linetreatment, between April 2000 and December 2008, were analyzed.Fourteen men and seventeen women, with a median age of 27 years,were submitted to autologous transplantation for relapsed (n = 21or refractory (n = 10 Hodgkin’s lymphoma. Results: Mortalityrelated to treatment in the first 100 days after transplant was 3.2%.With a mean follow-up period of 18 months (range: 1 to 88 months,the probability of global survival and progression-free survival in18 months was 84 and 80%, respectively. The probability of globalsurvival and progression-free survival at 18 months for patients withchemosensitive relapses (n = 21 was 95 and 90%, respectively,versus 60 and 45% for patients with relapses resistant to chemotherapy(n = 10 (p = 0.001 for global survival; p = 0.003 for progressionfreesurvival. In the multivariate analysis, absence of disease or pretransplant disease < 5 cm were favorable factors for global survival (p= 0.02; RR: 0.072; 95%CI: 0.01-0.85 and progression-free survival (p= 0.01; RR: 0.040; 95%CI: 0.007-0.78. Conclusion: Autologous transplantation of stem-cells is a therapeutic option for Hodgkin’s lymphoma patients after the first relapse. Promising results were observed in patients with a low tumor burden at transplant.

  11. Successful Treatment of Osgood–Schlatter Disease with Autologous-Conditioned Plasma in Two Patients

    OpenAIRE

    Danneberg, Dirk-Jonas

    2017-01-01

    Osgood–Schlatter Disease (OSD) is a painful, growth-related overuse condition of the tibial tuberosity, leading to inflammation of the patellar ligament at the tibial tuberosity. It primarily affects young adolescents, athletic population, and usually, resolves with age or skeletal maturity. Therapy is usually conservative, with surgery indicated in a minority of cases. For patients with treatment-resistant or refractory OSD, an alternative is the application of autologous platelet concentrat...

  12. Autologous Bone Marrow Transplantation in Multiple Sclerosis: Biomarker Relevance for Patient Recruitment and Follow up.

    Science.gov (United States)

    Londoño, Ana C; Mora, Carlos A

    2016-10-01

    Despite the current availability of disease modifying therapies for the treatment of multiple sclerosis, there are still patients who suffer from severe neurological dysfunction in the relapsing-remitting or early progressive forms of the disease. For these patients autologous hematopoietic stem cell transplant offers an important therapeutic solution to prevent progression to irreversible disability. In spite of multiple studies in the last two decades, patient inclusion criteria, protocols for peripheral blood stem cell mobilization and bone marrow cell conditioning and methodology of follow up for autologous hematopoietic stem cell transplant in multiple sclerosis have not been strictly unified. We reviewed five recent clinical studies that confirmed the positive outcome of transplant in spite of disclosing significant differences in methodology of enrollment including patient disease subtypes, disease duration range, disability, regimens of peripheral blood stem cell mobilization and bone marrow cell conditioning, scheduling of imaging studies after transplant, and absence of laboratory biomarkers consistently applied to these studies. Therapy with autologous hematopoietic stem cell transplant has shown best results among young individuals with severe relapsing-remitting or early progressive disease through its ability to maintain no evidence of disease activity status in a significantly higher proportion of patients after transplant in comparison to patients treated with disease modifying therapies. Important cross-sectional differences in the reviewed studies were found. A specific and careful selection of biomarkers, based on the current physiopathological mechanisms known to result in multiple sclerosis, will contribute to a better and earlier patient selection for autologous hematopoietic stem cell transplant and follow up process. An objective and measurable response could be obtained with the determination of biomarkers at the onset of treatment and

  13. Quantitative magnetic resonance imaging in autologous bone marrow transplantation for Hodgkin's disease.

    OpenAIRE

    Smith, S. R.; Williams, C. E.; Edwards, R. H.; Davies, J. M.

    1989-01-01

    Fifteen consecutive patients with refractory or relapsed Hodgkin's disease (HD) referred for autologous bone marrow transplantation (ABMT) underwent quantitative magnetic resonance (MR) studies of the lumbar vertebral bone marrow. Markedly elevated lumbar vertebral marrow T1 values suggestive of bone marrow involvement with HD were seen in four patients, two of whom had no evidence of HD on bilateral iliac crest bone marrow biopsy. Serial studies showed normalisation of T1 values in the post-...

  14. Autologous Stem Cell Transplantation in Patients with Acute Myeloid Leukemia: a Single-Centre Experience

    Directory of Open Access Journals (Sweden)

    Kakucs Enikő

    2013-04-01

    Full Text Available Introduction: Autologous haemopoietic stem cell transplantation (SCT is an important treatment modality for patients with acute myeloid leukemia with low and intermediate risk disease. It has served advantages over allogenic transplantation, because it does not need a matched donor, there is no graft versus host disease, there are less complications and a faster immune reconstitution than in the allo-setting. The disadvantage is the lack of the graft versus leukaemia effect.

  15. [Experimental study on bone defect treated by combined autologous bone marrow transplantation, cuttlebone, and sodium hyaluronate].

    Science.gov (United States)

    Yi, Hong-cheng; Tang, Liang-hua; Zhang, Xue-peng

    2011-08-01

    To study the feasibility of repairing bone defect by combined autologous bone marrow transplantation, cuttebone, and sodium hyaluronate. Forty-eight New Zealand rabbits were randomly divided into four groups. The 10-mm bone defect of the radial shaft animal model was established, with the periosteum remained. Rabbits of Group A were treated by autologous bone marrow transplantation, cuttlebone, and sodium hyaluronate. Those of Group B were treated by autologous bone marrow transplantation and cuttlebone. Rabbits of Group C were implanted with cuttlebone and sodium hyaluronate. And rabbits of Group D were taken as the blank control. There were twelve rabbits in each group. All rabbits were sacrificed, and the general histological examination, X-ray test, the pathohistological observation and scoring, the new born formation area measurement were performed at 2-week, 4-week, 8-week, and 12-week after transplantation respectively. The capacities for bone transplantation and defect repairing were compared and analyzed as well. The bone defect of Group A was completely repaired at week 12. The comprehensive indices at each time point were superior to those of the rest groups, showing statistical significance (Pbone repair in Group B and Group C were somewhat poor, with the repairing effect inferior to that of Group A. The bone repairing was better in Group B than in Group C. Most portion of the bone defect in Group D was filled with fibrous tissue and muscular tissue, with little bone repairing. The combined autologous bone marrow transplantation, cuttlebone, and sodium hyaluronate showed obviously synergistically bone forming capacities. It could be taken as a substitute material for transplantation.

  16. Differentiation within autologous fibrin scaffolds of porcine dermal cells with the mesenchymal stem cell phenotype

    International Nuclear Information System (INIS)

    Puente, Pilar de la; Ludeña, Dolores; López, Marta; Ramos, Jennifer; Iglesias, Javier

    2013-01-01

    Porcine mesenchymal stem cells (pMSCs) are an attractive source of cells for tissue engineering because their properties are similar to those of human stem cells. pMSCs can be found in different tissues but their dermal origin has not been studied in depth. Additionally, MSCs differentiation in monolayer cultures requires subcultured cells, and these cells are at risk of dedifferentiation when implanting them into living tissue. Following this, we attempted to characterize the MSCs phenotype of porcine dermal cells and to evaluate their cellular proliferation and differentiation in autologous fibrin scaffolds (AFSs). Dermal biopsies and blood samples were obtained from 12 pigs. Dermal cells were characterized by flow cytometry. Frozen autologous plasma was used to prepare AFSs. pMSC differentiation was studied in standard structures (monolayers and pellets) and in AFSs. The pMSCs expressed the CD90 and CD29 markers of the mesenchymal lineage. AFSs afforded adipogenic, osteogenic and chondrogenic differentiation. The porcine dermis can be proposed to be a good source of MSCs with adequate proliferative capacity and a suitable expression of markers. The pMSCs also showed optimal proliferation and differentiation in AFSs, such that these might serve as a promising autologous and implantable material for use in tissue engineering. -- Highlights: ► Low fibrinogen concentration provides a suitable matrix for cell migration and differentiation. ► Autologous fibrin scaffolds is a promising technique in tissue engineering. ► Dermal cells are an easily accessible mesenchymal stem cell source. ► Fibrin scaffolds afforded adipogenic, osteogenic and chondrogenic differentiation.

  17. Use of autologous pericardium for mitral leaflet reconstruction in a child with endocarditis

    Directory of Open Access Journals (Sweden)

    Olcay Murat Disli

    2013-06-01

    Full Text Available We present a case of successful repair of the mitral valve for active infective endocarditis. Mitral valve repair was performed through debridement of vegetation and abscess, resection and repair of the posterior mitral leaflet and posterior repair with autologous pericardium. Postoperative period was uneventfully, with no evidence of recurrent infection, and echocardiogram showed mitral valve competence with mild mitral regurgitation. We demonstrate that valve repair is a feasible choice in cases of active endocarditis in children.

  18. Funding analysis of bilateral autologous free-flap breast reconstructions in Australia.

    Science.gov (United States)

    Sinha, Shiba; Ruskin, Olivia; McCombe, David; Morrison, Wayne; Webb, Angela

    2015-08-01

    Bilateral breast reconstructions are being increasingly performed. Autologous free-flap reconstructions represent the gold standard for post-mastectomy breast reconstruction but are resource intensive. This study aims to investigate the difference between hospital reimbursement and true cost of bilateral autologous free-flap reconstructions. Retrospective analysis of patients who underwent bilateral autologous free-flap reconstructions at a single Australian tertiary referral centre was performed. Hospital reimbursement was determined from coding analysis. A true cost analysis was also performed. Comparisons were made considering the effect of timing, indication and complications of the procedure. Forty-six bilateral autologous free-flap procedures were performed (87 deep inferior epigastric perforators (DIEPs), four superficial inferior epigastric artery perforator flaps (SIEAs) and one muscle-sparing free transverse rectus abdominis myocutaneous flap (MS-TRAM)). The mean funding discrepancy between hospital reimbursement and actual cost was $12,137 ± $8539 (mean ± standard deviation (SD)) (n = 46). Twenty-four per cent (n = 11) of the cases had been coded inaccurately. If these cases were excluded from analysis, the mean funding discrepancy per case was $9168 ± $7453 (n = 35). Minor and major complications significantly increased the true cost and funding discrepancy (p = 0.02). Bilateral free-flap breast reconstructions performed in Australian public hospitals result in a funding discrepancy. Failure to be economically viable threatens the provision of this procedure in the public system. Plastic surgeons and hospital managers need to adopt measures in order to make these gold-standard procedures cost neutral. Copyright © 2015 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.

  19. Differentiation within autologous fibrin scaffolds of porcine dermal cells with the mesenchymal stem cell phenotype

    Energy Technology Data Exchange (ETDEWEB)

    Puente, Pilar de la, E-mail: pilardelapuentegarcia@gmail.com [Tissue Bank, San Francisco Clinic Foundation, Av./Facultad 51, 5°, 24004 León (Spain); Ludeña, Dolores [Pathology Service, University Hospital of Salamanca, P/San Vicente 58-182, 37007 Salamanca (Spain); López, Marta; Ramos, Jennifer; Iglesias, Javier [Tissue Bank, San Francisco Clinic Foundation, Av./Facultad 51, 5°, 24004 León (Spain)

    2013-02-01

    Porcine mesenchymal stem cells (pMSCs) are an attractive source of cells for tissue engineering because their properties are similar to those of human stem cells. pMSCs can be found in different tissues but their dermal origin has not been studied in depth. Additionally, MSCs differentiation in monolayer cultures requires subcultured cells, and these cells are at risk of dedifferentiation when implanting them into living tissue. Following this, we attempted to characterize the MSCs phenotype of porcine dermal cells and to evaluate their cellular proliferation and differentiation in autologous fibrin scaffolds (AFSs). Dermal biopsies and blood samples were obtained from 12 pigs. Dermal cells were characterized by flow cytometry. Frozen autologous plasma was used to prepare AFSs. pMSC differentiation was studied in standard structures (monolayers and pellets) and in AFSs. The pMSCs expressed the CD90 and CD29 markers of the mesenchymal lineage. AFSs afforded adipogenic, osteogenic and chondrogenic differentiation. The porcine dermis can be proposed to be a good source of MSCs with adequate proliferative capacity and a suitable expression of markers. The pMSCs also showed optimal proliferation and differentiation in AFSs, such that these might serve as a promising autologous and implantable material for use in tissue engineering. -- Highlights: ► Low fibrinogen concentration provides a suitable matrix for cell migration and differentiation. ► Autologous fibrin scaffolds is a promising technique in tissue engineering. ► Dermal cells are an easily accessible mesenchymal stem cell source. ► Fibrin scaffolds afforded adipogenic, osteogenic and chondrogenic differentiation.

  20. Use of autologous bone graft in anterior cervical decompression: morbidity & quality of life analysis.

    LENUS (Irish Health Repository)

    Heneghan, Helen M

    2009-01-01

    BACKGROUND: Autologous iliac crest graft has long been the gold standard graft material used in cervical fusion. However its harvest has significant associated morbidity, including protracted postoperative pain scores at the harvest site. Thus its continued practice warrants scrutiny, particularly now that alternatives are available. Our aims were to assess incidence and nature of complications associated with iliac crest harvest when performed in the setting of Anterior Cervical Decompression (ACD). Also, to perform a comparative analysis of patient satisfaction and quality of life scores after ACD surgeries, when performed with and without iliac graft harvest. METHODS: All patients who underwent consecutive ACD procedures, with and without the use of autologous iliac crest graft, over a 48 month period were included (n = 53). Patients were assessed clinically at a minimum of 12 months postoperatively and administered 2 validated quality of life questionnaires: the SF-36 and Cervical Spine Outcomes Questionnaires (Response rate 96%). Primary composite endpoints included incidence of bone graft donor site morbidity, pain scores, operative duration, and quality of life scores. RESULTS: Patients who underwent iliac graft harvest experienced significant peri-operative donor site specific morbidity, including a high incidence of pain at the iliac crest (90%), iliac wound infection (7%), a jejunal perforation, and longer operative duration (285 minutes vs. 238 minutes, p = 0.026). Longer term follow-up demonstrated protracted postoperative pain at the harvest site and significantly lower mental health scores on both quality of life instruments, for those patients who underwent autologous graft harvest CONCLUSION: ACD with iliac crest graft harvest is associated with significant iliac crest donor site morbidity and lower quality of life at greater than 12 months post operatively. This is now avoidable by using alternatives to autologous bone without compromising clinical

  1. Clinical application of tissue engineered human heart valves using autologous progenitor cells.

    Science.gov (United States)

    Cebotari, Serghei; Lichtenberg, Artur; Tudorache, Igor; Hilfiker, Andres; Mertsching, Heike; Leyh, Rainer; Breymann, Thomas; Kallenbach, Klaus; Maniuc, Liviu; Batrinac, Aurel; Repin, Oleg; Maliga, Oxana; Ciubotaru, Anatol; Haverich, Axel

    2006-07-04

    Tissue engineering (TE) of heart valves reseeded with autologous cells has been successfully performed in vitro. Here, we report our first clinical implantation of pulmonary heart valves (PV) engineered with autologous endothelial progenitor cells (EPCs) and the results of 3.5 years of follow-up. Human PV allografts were decellularized (Trypsin/EDTA) and resulting scaffolds reseeded with peripheral mononuclear cells isolated from human blood. Positive stain for von Willebrand factor, CD31, and Flk-1 was observed in monolayers of cells cultivated and differentiated on the luminal surface of the scaffolds in a dynamic bioreactor system for up to 21 days, indicating endothelial nature. PV reseeded with autologous cells were implanted into 2 pediatric patients (age 13 and 11) with congenital PV failure. Postoperatively, a mild pulmonary regurgitation was documented in both children. Based on regular echocardiographic investigations, hemodynamic parameters and cardiac morphology changed in 3.5 years as follows: increase of the PV annulus diameter (18 to 22.5 mm and 22 to 26 mm, respectively), decrease of valve regurgitation (trivial/mild and trivial, respectively), decrease (16 to 9 mm Hg) or a increase (8 to 9.5 mm Hg) of mean transvalvular gradient, remained 26 mm or decreased (32 to 28 mm) right-ventricular end-diastolic diameter. The body surface area increased (1.07 to 1.42 m2 and 1.07 to 1.46 m2, respectively). No signs of valve degeneration were observed in both patients. TE of human heart valves using autologous EPC is a feasible and safe method for pulmonary valve replacement. TE valves have the potential to remodel and grow accordingly to the somatic growth of the child.

  2. Treatment of periodontal intrabony defects using autologous periodontal ligament stem cells: a randomized clinical trial

    OpenAIRE

    Chen, Fa-Ming; Gao, Li-Na; Tian, Bei-Min; Zhang, Xi-Yu; Zhang, Yong-Jie; Dong, Guang-Ying; Lu, Hong; Chu, Qing; Xu, Jie; Yu, Yang; Wu, Rui-Xin; Yin, Yuan; Shi, Songtao; Jin, Yan

    2016-01-01

    Background Periodontitis, which progressively destroys tooth-supporting structures, is one of the most widespread infectious diseases and the leading cause of tooth loss in adults. Evidence from preclinical trials and small-scale pilot clinical studies indicates that stem cells derived from periodontal ligament tissues are a promising therapy for the regeneration of lost/damaged periodontal tissue. This study assessed the safety and feasibility of using autologous periodontal ligament stem ce...

  3. Autologous Transplantation of Amniotic Fluid-Derived Mesenchymal Stem Cells into Sheep Fetuses.

    Science.gov (United States)

    Shaw, S W Steven; Bollini, Sveva; Nader, Khalil Abi; Gastaldello, Annalisa; Mehta, Vedanta; Filppi, Elisa; Cananzi, Mara; Gaspar, H Bobby; Qasim, Waseem; De Coppi, Paolo; David, Anna L

    2016-03-01

    Long-term engraftment and phenotype correction has been difficult to achieve in humans after in utero stem cell transplantation mainly because of allogeneic rejection. Autologous cells could be obtained during gestation from the amniotic fluid with minimal risk for the fetus and the mother. Using a sheep model, we explored the possibility of using amniotic fluid mesenchymal stem cells (AFMSCs) for autologous in utero stem cell/gene therapy. We collected amniotic fluid (AF) under ultrasound-guided amniocentesis in early gestation pregnant sheep ( n = 9, 58 days of gestation, term = 145 days). AFMSCs were isolated and expanded in all sampled fetal sheep. Those cells were transduced using an HIV vector encoding enhanced green fluorescent protein (GFP) with 63.2% (range 38.3-96.2%) transduction efficiency rate. After expansion, transduced AFMSCs were injected into the peritoneal cavity of each donor fetal sheep at 76 days under ultrasound guidance. One ewe miscarried twin fetuses after amniocentesis. Intraperitoneal injection was successful in the remaining 7 fetal sheep giving a 78% survival for the full procedure. Tissues were sampled at postmortem examination 2 weeks later. PCR analysis detected GFP-positive cells in fetal tissues including liver, heart, placenta, membrane, umbilical cord, adrenal gland, and muscle. GFP protein was detected in these tissues by Western blotting and further confirmed by cytofluorimetric and immunofluorescence analyses. This is the first demonstration of autologous stem cell transplantation in the fetus using AFMSCs. Autologous cells derived from AF showed widespread organ migration and could offer an alternative way to ameliorate prenatal congenital disease.

  4. Arthroscopic osteochondral autologous transplantation for the treatment of osteochondritis dissecans of the femoral head

    Directory of Open Access Journals (Sweden)

    Uchida Soshi

    2017-01-01

    Full Text Available Osteochondritis dissecans (OCD of the femoral head is an unusual cause of hip pain. It can be associated with other intra-articular pathologies including: acetabular labral tears or bone deformities such as Legg-Calve-Perthes Disease (LCPD. In this article, we propose a modern surgical technique using an arthroscopic antegrade and retrograde osteochondral autologous transplantation (OAT procedure for assessing and treating OCD lesions of the femoral head.

  5. Deutsch als Fremdsprache im Tourismus: Empirische Untersuchungen zur Kommunikation in der Tourismusbranche

    Directory of Open Access Journals (Sweden)

    Doğu Ataş

    2017-12-01

    Full Text Available Die vorliegende Studie definiert diejenigen Sprechintentionen, die zwischen deutschsprachigen Touristen und türkischen Beschäftigten in der Tourismusbranche wie Kellner, Kundenbeziehungsmanager, Rezeptionist und Animateur vielfach vorkommen. Ein Teil der Daten für die Untersuchung wurde im Rahmen einer qualitativen Forschungsarbeit mittels teilstandardisierten Interviews und schriftlicher Befragung mit offenen Fragen erhoben, ein weiterer Teil Anhand einer quantitativen Forschungsarbeit mittels schriftlicher Befragung mit Fragebogen. Die offenen Fragen wurden den deutschsprachigen Personen gestellt, die mindestens einmal ihre Ferien als Tourist in der Türkei verbrachten. Dagegen wurden die Fragebogenstudie und die Interviews mit türkischen Mitarbeitern im Tourismusbereich durchgeführt. Die erhobenen Daten aus den Interviews und der offenen Fragen wurden mittels qualitativer Inhaltsanalyse ausgewertet. Im Vergleich dazu wurde die deskriptive Statistik zur Auswertung der Fragebogenstudie herangezogen. Die erfassten Sprechintentionen können für die Entwicklung eines tourismusbezogenen DaF-Lehrwerks (Deutsch als Fremdsprache verwendet werden, das insbesondere den Bedürfnissen der türkischen Adressatengruppe entspricht.

  6. Evaluation von Bildungsportalen – Empirische Untersuchungen zur Nutzung des Deutschen Bildungsservers

    Directory of Open Access Journals (Sweden)

    Elke Brenstein

    2017-08-01

    Full Text Available Die Produktion von Wissen wird immer schneller, und die technischen Möglichkeiten der IKT, Informationen zu vervielfältigen, zu verbreiten und zugänglich zu machen, wachsen ständig. Informationsflut und Wissensexplosion erfordern deshalb einen intelligenten und verantwortungsvollen Umgang mit komplexen und vielfältig vernetzten Wissensinhalten. Der Deutsche Bildungsserver und das Schulweb sind zwei stark frequentierte Internetportale, welche inhaltlich hochwertige und wissenschaftlich strukturierte Informationen zum Thema Bildung anbieten. Obwohl sich die Angebote einer ständig wachsenden Nutzung erfreuen, ist es für die inhaltliche und strukturelle Weiterentwicklung der Angebotspalette wichtig zu wissen, wer die Nutzer sind und wie die Informationen von unterschiedlichen Nutzergruppen angenommen und bewertet werden. Diese Fragestellungen wurden mit quantitativen und qualitativen Erhebungsmethoden untersucht. Im Folgenden werden exemplarische Ergebnisse von Online-Befragungen und Fokusgruppenuntersuchungen zu inhaltlichen, strukturellen und graphischen Aspekten der Gebrauchstauglichkeit (Usability dargestellt. Darüber hinaus werden die Teilergebnisse einer Detailauswertung von Logfiledaten eines Jahres präsentiert. Aus den Ergebnissen werden Empfehlungen zur nutzergruppenspezifischen Optimierung der Angebote abgeleitet.

  7. Einstellung und Wissen von Lehramtsstudierenden zur Evolution - ein Vergleich zwischen Deutschland und der Türkei

    Science.gov (United States)

    Graf, Dittmar; Soran, Haluk

    Es wird eine Untersuchung vorgestellt, in der Wissen und Überzeugungen von Lehramtsstudierenden aller Fächer zum Thema Evolution an zwei Universitäten in Deutschland und der Türkei erhoben worden sind. Die Befragung wurde in Dortmund und in Ankara durchgeführt. Es stellte sich heraus, dass ausgeprägte Defizite im Verständnis der Evolutionsmechanismen herrschen. Viele Studierende, insbesondere aus der Türkei, sind nicht von der Faktizität der Evolution überzeugt. Dies gilt sowohl für Studierende mit Fach Biologie als auch für Studierende mit anderen Fächern. Näher untersucht worden sind die Faktoren, die die Überzeugungen zur Evolution beeinflussen können, was ja in Anbetracht der hohen Ablehnungsrate der Evolution von besonderem Interesse ist. Das Vertrauen in die Wissenschaft spielt hierbei eine besondere Rolle: Wer der Wissenschaft vertraut, ist auch eher von der Evolution überzeugt, als diejenigen, die skeptisch gegenüber der Wissenschaft sind.

  8. [COPD und Klangtherapie: Pilotstudie zur Wirksamkeit einer Behandlung mit Körpertambura bei COPD-Patienten].

    Science.gov (United States)

    Hartwig, Bernhard; Schmidt, Stefan; Hartwig, Isabella

    2016-01-01

    Hintergrund: Erkrankungen der Atemorgane treten mit steigendem Alter öfter auf, nehmen weltweit zu und sind häufige Ursachen für Morbidität und Mortalität. In dieser Pilotstudie wurde der Frage nachgegangen, ob eine einmalige 10-minütige Behandlung mit einer Körpertambura eine signifikante und effektive Verbesserung der Lungenfunktion von Patienten mit chronisch-obstruktiver Lungenerkrankung (COPD; GOLD-Stadium A oder B) erbringen kann. Patienten und Methoden: 54 Probanden konnten je zur Hälfte in eine Behandlungsgruppe (Körpertambura) und eine aktive Kontrollgruppe (Atemtherapie) randomisiert werden. Eine Bestimmung der Lungenfunktionsmessparameter «Einsekundenkapazität» (FEV1) und «inspiratorische Vitalkapazität» (IVC) zu den Zeitpunkten T1 (Baseline), T2 (direkt nach Behandlung) und als Follow-up etwa 3 Wochen nach T1 (T3). Ergebnisse: Die Behandlungsgruppe zeigte sich der Kontrollgruppe in beiden Werten signifikant überlegen. Die Zeit-×-Gruppe-Interaktion (Varianzanalyse) ergab p = 0,001 (FEV1) bzw. p = 0,04 (IVC). Die Behandlungsgruppe zeigte bei beiden Werten eine Verbesserung von klinischer Relevanz. Schlussfolgerung: Diese Ergebnisse zeigen, dass die Klangbehandlung mittels einer Körpertambura - neben den schulmedizinischen, leitliniengerechten Therapien - eine zusätzliche, nebenwirkungsarme, aber durchaus klinisch wirksame Option für die Behandlung von COPD-Patienten darstellen kann, um deren Lebensqualität zu stabilisieren und zu verbessern. © 2016 S. Karger GmbH, Freiburg.

  9. Ganzheitliche Digitalisierungsansätze im Stadtwerk: Von der Strategie bis zur Umsetzung

    Science.gov (United States)

    Dudenhausen, Roman; Hahn, Heike

    Digitalisierung muss im Stadtwerk dazu führen, Kundenerwartungen, die heutzutage schon vielfach durch digitales Know-how und Erfahrungen geprägt sind, in einzigartiger Weise zu entsprechen - in Form digitaler Kundenkontaktpunkte, automatisierter Prozesse oder plattformbasierter Geschäftsmodelle. Eine große Rolle spielen dabei unternehmensweit nutzbare Informationen, die eine 360-Grad-Sicht auf den Kunden ermöglichen. Nur in dieser Kombination werden sich nachhaltig Wettbewerbsvorteile generieren lassen. Manch ein Kunde wird die Lust, einen Prozess zu Ende zu gehen, schon vor dem Abschluss verlieren, wenn er nicht unmittelbar und ohne die digitale Welt zu verlassen zum Ziel kommt. Eine nur "halb digitale Kundenerfahrung" wird weder zu Neugeschäft noch zur positiven emotionalen Bindung zwischen Kunden und Stadtwerk führen. Nicht zu unterschätzen sind zudem Erwartungen hinsichtlich zukünftiger Geschäftsmodelle, aus denen sich disruptive Bedrohungen für die herkömmlichen Strom- und Gasangebote ergeben werden. Erste innovative Ansätze finden sich bereits im Markt, die erahnen lassen, dass zurzeit viel diskutierte Technologien wie die Blockchain nicht mehr nur hypothetischer Natur sind. Die Auseinandersetzung mit der Digitalisierung erfolgt dabei sinnvollerweise in einem unternehmensweit abgestimmten Rahmen, der eine zielgerichtete und ganzheitliche Vorgehensweise ermöglicht.

  10. Gabriele Winker, Nina Degele: Intersektionalität. Zur Analyse sozialer Ungleichheiten. Bielefeld: transcript Verlag 2009.

    Directory of Open Access Journals (Sweden)

    Heike Kahlert

    2010-03-01

    Full Text Available Gabriele Winker und Nina Degele legen mit ihrer kompakten Monographie erstmals für den deutschsprachigen Raum eine in Theorie, Methodologie und Empirie stringente eigenständige Abhandlung zur intersektionalen Analyse von sozialen Ungleichheiten vor. Das in jeder Hinsicht übersichtlich gestaltete Buch besticht durch eine begründete Argumentation, insbesondere in Bezug auf die theoretischen Vorentscheidungen, durch eine klare Gliederung und vor allem durch die Schritt für Schritt nachvollziehbare Umsetzung des vorgeschlagenen theoretischen und methodologischen Rahmens in die empirische Sozialforschung. Es ist daher uneingeschränkt empfehlenswert, auch für den Einsatz in der Hochschullehre, und es ist von hohem Anregungspotential für die wissenschaftliche Weiterentwicklung intersektionaler Analysen von sozialen Ungleichheiten.This compact monograph by Gabriele Winker and Nina Degele, intended for a German-speaking readership, provides a theoretically, methodologically, and empirically stringent as well as independent critique of intersectional analysis of social inequalities. The book, which is in every sense clearly organized, is striking because of its well-founded argumentation (especially in terms of the authors’ theoretical decisions, its clear structure, and most notably its step-by-step and comprehensible application of the suggested theoretical and methodological framework for empirical social research. The book is thus recommended without reserve, also for use in higher education, and it has the potential to inspire further scientific development of intersectional analysis of social inequities.

  11. Autologous Bone Marrow-Derived Stem Cells in Spinal Cord Injury.

    Science.gov (United States)

    Bansal, Himanshu; Verma, Poonam; Agrawal, Anupama; Leon, Jerry; Sundell, I Birgitta; Koka, Prasad S

    Spinal cord injury is a traumatic neurological condition which makes the patient disable. Its management still remains challenging but advancements in the regenerative medicine have changed the approach of treating this serious debilitating condition of the central nervous system. Cell based therapies can restore function in spinal cord injury by replacing the lost neural tissue. These therapies also rejuvenate the existing intact neurons by facilitating remyelination and by repairing and reducing progressive tissue damage and scarring. Autologous bone marrow stem cells were collected from the patients. 5 ml of the processed sample was injected back into the patients via lumbar puncture at L1/L2 level. The bone marrow harvesting and administration was repeated every 4 weeks 3 times (12 weeks). Significant improvements were noticed following the injections into the patients with the duration of injury less than 6 months. ASIA grade improvements were observed in 6 out of 10 patients. VTC and walking, at least with the support, was restored in eight patients. Bladder control and sexual functions improved in three and five patients respectively. Eight patients exhibited decreased spasticity. We believe that autologous bone marrow stem cells contributed towards the neuroplaticity and/or paracrine effect due to which we observed the considerable improvements in the conditions of the patients. This preliminary proof of patient improvement reinforces the potential of autologous bone marrow stem cell treatment in the patients suffering from Spinal Cord Injury. Although the results are encouraging further studies are needed to substantiate the claims.

  12. Intrasphincteric autologous myoblast injections with electrical stimulation for stress urinary incontinence.

    Science.gov (United States)

    Blaganje, Mija; Lukanović, Adolf

    2012-05-01

    To assess the feasibility and safety of ultrasound-guided autologous myoblast injections into the external urethral sphincter followed by electrical stimulation (ES) as a possible 2-step treatment for stress urinary incontinence (SUI). Autologous myoblasts isolated from a biceps muscle sample were injected under transurethral ultrasound guidance into the external urethral sphincter of 38 female patients. The patients also underwent ES postoperatively to enhance cell integration. Treatment feasibility, as well as possible intraoperative and postoperative complications, was assessed 6weeks after the injections. Additionally, the effects of the myoblast injections followed by an ES cycle were compared to those of a preoperative ES cycle undergone by the same patients. No serious adverse events or complications were noted and the procedure was well tolerated. Compared with the objective and subjective measurements collected after the preoperative ES cycle, the corresponding measurements obtained 6weeks postoperatively, after the completion of a second ES cycle, indicated considerable improvement. The results to the stress test were negative for 29 (78.4%) of the patients, 5 (13.5%) considered their SUI cured, and 29 (78.4%) reported improvement. Intrasphincteric autologous myoblast injections followed by ES is minimally invasive and feasible, and safely produced promising initial results. EU Clinical Trials EudraCT No. 2009-012389-30 ClinicalTrials.gov identifier: NCT01355133. Copyright © 2012 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.

  13. A successful case of a para-aortic lymphocele treated with autologous peripheral blood injection

    Directory of Open Access Journals (Sweden)

    Keiji Nishibeppu, MD

    2017-12-01

    Full Text Available A lymphocele is one of the complications of systematic pelvic or para-aortic lymphadenectomy. Although most patients are entirely asymptomatic, our patient exhibited an obstructive ileus at the jejunum compressed by a lymphocele. We report here a case of a subsequent para-aortic lymphocele treated with autologous peripheral blood injection. A 68-year-old woman with sigmoid colon cancer (T3N2bM1a underwent laparoscopic sigmoidectomy. After 4 courses of chemotherapy (CapeOX + Bmab, para-aortic lymphadenectomy was additionally performed. One month later, an obstructive ileus occurred suddenly due to a lymphocele. A drainage catheter was placed into the lymphocele and a total of 35 mL of autologous peripheral blood was injected in 4 divided doses through the catheter. The volume of the lymphocele gradually reduced and the ileus improved after blood injection. This is the first report of a successful case of a subsequent para-aortic lymphocele treated with autologous peripheral blood injection without any complications.

  14. Medialization Thyroplasty Using Autologous Nasal Septal Cartilage for Treating Unilateral Vocal Fold Paralysis

    Science.gov (United States)

    Khalil, Yasser A.; Malki, Khalid H.; Farahat, Mohamad

    2011-01-01

    Objectives A persistent insufficiency of glottal closure is mostly a consequence of impaired unilateral vocal fold movement. Functional surgical treatment is required because of the consequential voice, breathing and swallowing impairments. The goal of the study was to determine the functional voice outcomes after medialization thyroplasty with using autologous septal cartilage from the nose. Methods External vocal fold medialization using autologous nasal septal cartilage was performed on 15 patients (6 females and 9 males; age range, 30 to 57 years). Detailed functional examinations were performed for all the patients before and after the surgery and this included perceptual voice assessment, laryngostroboscopic examination and acoustic voice analysis. Results All the patients reported improvement of voice quality post-operatively. Laryngostroboscopy revealed almost complete glottal closure after surgery in the majority of patients. Acoustic and perceptual voice assessment showed significant improvement post-operatively. Conclusion Medialization thyroplasty using an autologous nasal septal cartilage implant offers good tissue tolerability and significant improvement of the subjective and objective functional voice outcomes. PMID:21949581

  15. Comparison of Complications Following Cranioplasty Using a Sterilized Autologous Bone Flap or Polymethyl Methacrylate.

    Science.gov (United States)

    Kim, Sung Hoon; Kang, Dong Soo; Cheong, Jin Hwan; Kim, Jung Hee; Song, Kwan Young; Kong, Min Ho

    2017-04-01

    The aims of current study are to compare complications following cranioplasty (CP) using either sterilized autologous bone or polymethyl methacrylate (PMMA), and to identify the risk factors for two of the most common complications: bone flap resorption (BFR) and surgical site infection (SSI). Between January 2004 and December 2013, 127 patients underwent CP and were followed at least 12 months. Variables, including sex, age, initial diagnosis, time interval between decompressive craniectomy (DC) and CP, operation time, size of bone flap, and presence of ventriculo-peritoneal shunt, were analyzed to identify the risk factors for BFR and SSI. A total of 97 (76.4%) patients underwent CP using PMMA (Group I) and 30 (23.6%) underwent CP using autologous bone (Group II). SSI occurred in 8 (8.2%) patients in Group I, and in 2 (6.7%) in Group II; there was no statistically significant difference between the groups ( p =1.00). No statistically significant risk factors for SSI were found in either group. In Group I, there was no reported case of BFR. In Group II patients, BFR developed in 18 (60.0%) patients at the time of CP (Type 1 BFR), and at 12-month follow up (Type 2 BFR) in 4 (13.3%) patients. No statistically significant risk factors for BFR were found in Group II. CP using sterilized autologous bone result in a significant rate of BFR. PMMA, however, is a safe alloplastic material for CP, as it has low complication rate.

  16. Autologous serum and plasma skin tests in chronic spontaneous urticaria: A reappraisal

    Directory of Open Access Journals (Sweden)

    Muthu Sendhil Kumaran

    2017-01-01

    Full Text Available Aim: The objective of this study was to assess autologous serum skin test (ASST vs autologous plasma skin test (APST response in chronic spontaneous urticaria (CSU patients and study the significance of intensity of positive responses in relation to clinicoepidemiological parameters. Materials and Methods: One hundred CSU patients and 100 age and sex-matched controls were recruited. The demographic and clinical features were recorded in all patients and routine investigations were performed. ASST and APST tests were performed as per the standard guidelines. Results: The mean duration of illness was 4.85 ± 5.07 years, 90% patients were APST (+, 68% ASST (+, and 22 patients were only APST (+. Positive predictive value (PPV of ASST and APST was 90.7% and 95.7%, respectively. A significant inverse association was seen between thyroid status and serum IgE levels with APST and ASST positivity. Conclusion: APST appears to have better PPV and high intensity of positive response on autologous tests, and correlates with ANA positivity and angioedema.

  17. Preclinical derivation and imaging of autologously transplanted canine induced pluripotent stem cells.

    Science.gov (United States)

    Lee, Andrew S; Xu, Dan; Plews, Jordan R; Nguyen, Patricia K; Nag, Divya; Lyons, Jennifer K; Han, Leng; Hu, Shijun; Lan, Feng; Liu, Junwei; Huang, Mei; Narsinh, Kazim H; Long, Charles T; de Almeida, Patricia E; Levi, Benjamin; Kooreman, Nigel; Bangs, Charles; Pacharinsak, Cholawat; Ikeno, Fumiaki; Yeung, Alan C; Gambhir, Sanjiv S; Robbins, Robert C; Longaker, Michael T; Wu, Joseph C

    2011-09-16

    Derivation of patient-specific induced pluripotent stem cells (iPSCs) opens a new avenue for future applications of regenerative medicine. However, before iPSCs can be used in a clinical setting, it is critical to validate their in vivo fate following autologous transplantation. Thus far, preclinical studies have been limited to small animals and have yet to be conducted in large animals that are physiologically more similar to humans. In this study, we report the first autologous transplantation of iPSCs in a large animal model through the generation of canine iPSCs (ciPSCs) from the canine adipose stromal cells and canine fibroblasts of adult mongrel dogs. We confirmed pluripotency of ciPSCs using the following techniques: (i) immunostaining and quantitative PCR for the presence of pluripotent and germ layer-specific markers in differentiated ciPSCs; (ii) microarray analysis that demonstrates similar gene expression profiles between ciPSCs and canine embryonic stem cells; (iii) teratoma formation assays; and (iv) karyotyping for genomic stability. Fate of ciPSCs autologously transplanted to the canine heart was tracked in vivo using clinical positron emission tomography, computed tomography, and magnetic resonance imaging. To demonstrate clinical potential of ciPSCs to treat models of injury, we generated endothelial cells (ciPSC-ECs) and used these cells to treat immunodeficient murine models of myocardial infarction and hindlimb ischemia.

  18. Bone marrow concentrate for autologous transplantation in minipigs. Characterization and osteogenic potential of mesenchymal stem cells.

    Science.gov (United States)

    Herten, M; Grassmann, J P; Sager, M; Benga, L; Fischer, J C; Jäger, M; Betsch, M; Wild, M; Hakimi, M; Jungbluth, P

    2013-01-01

    Autologous bone marrow plays an increasing role in the treatment of bone, cartilage and tendon healing disorders. Cell-based therapies display promising results in the support of local regeneration, especially therapies using intra-operative one-step treatments with autologous progenitor cells. In the present study, bone marrow-derived cells were concentrated in a point-of-care device and investigated for their mesenchymal stem cell (MSC) characteristics and their osteogenic potential. Bone marrow was harvested from the iliac crest of 16 minipigs. The mononucleated cells (MNC) were concentrated by gradient density centrifugation, cultivated, characterized by flow cytometry and stimulated into osteoblasts, adipocytes, and chondrocytes. Cell differentiation was investigated by histological and immunohistological staining of relevant lineage markers. The proliferation capacity was determined via colony forming units of fibroblast and of osteogenic alkaline-phosphatase-positive-cells. The MNC could be enriched 3.5-fold in nucleated cell concentrate in comparison to bone marrow. Flow cytometry analysis revealed a positive signal for the MSC markers. Cells could be differentiated into the three lines confirming the MSC character. The cellular osteogenic potential correlated significantly with the percentage of newly formed bone in vivo in a porcine metaphyseal long-bone defect model. This study demonstrates that bone marrow concentrate from minipigs display cells with MSC character and their osteogenic differentiation potential can be used for osseous defect repair in autologous transplantations.

  19. [Long-term results of intracoronary transplantation of autologous bone marrow cells in dilated cardiomyopathy].

    Science.gov (United States)

    Bartolucci, Jorge; Verdugo, Fernando J; Carrion, Flavio; Abarzúa, Ema; Goset, Carlos; Lamich, Rubén; Sanhueza, Patricio; Pedreros, Pablo; Nazzal, Carolina; Khoury, Maroun; Figueroa, Fernando E

    2015-04-01

    Intracoronary delivery of autologous bone marrow mononuclear cells is an interesting therapeutic promise for patients with heart failure of different etiologies. To evaluate the long-term safety and efficacy of this therapy in patients with dilated cardiomyopathy of different etiologies under optimal medical treatment. Prospective, open-label, controlled clinical trial. Of 23 consecutive patients, 12 were assigned to autologous bone marrow mononuclear cell intracoronary transplantation, receiving a mean dose of 8.19 ± 4.43 x 10(6) CD34+ cells. Mortality, cardiovascular readmissions and cancer incidence rate, changes in functional capacity, quality of life questionnaires and echocardiographic measures from baseline, were assessed at long-term follow-up (37.7 ± 9.7 months) in patients receiving or not the cells. No significant differences were observed in mortality, cardiovascular readmissions or cancer incidence rate amongst groups. An improvement in functional class and quality of life questionnaires in the transplanted group was observed (p transplantation of autologous bone marrow mononuclear cells is feasible and safe in patients with dilated cardiomyopathy of diverse etiologies. This therapy was associated to persistent improvements in functional class and quality of life. There was also a non-significant long-term improvement of left ventricular function.

  20. Second-degree burns with six etiologies treated with autologous noncultured cell-spray grafting.

    Science.gov (United States)

    Esteban-Vives, Roger; Choi, Myung S; Young, Matthew T; Over, Patrick; Ziembicki, Jenny; Corcos, Alain; Gerlach, Jörg C

    2016-11-01

    Partial and deep partial-thickness burn wounds present a difficult diagnosis and prognosis that makes the planning for a conservative treatment versus mesh grafting problematic. A non-invasive treatment strategy avoiding mesh grafting is often chosen by practitioners based on their clinical and empirical evidence. However, a delayed re-epithelialization after conservative treatment may extend the patient's hospitalization period, increase the risk of infection, and lead to poor functional and aesthetic outcome. Early spray grafting, using non-cultured autologous cells, is under discussion for partial and deep partial-thickness wounds to accelerate the re-epithelialization process, reducing the healing time in the hospital, and minimizing complications. To address planning for future clinical studies on this technology, suitable indications will be interesting. We present case information on severe second-degree injuries after gas, chemical, electrical, gasoline, hot water, and tar scalding burns showing one patient per indication. The treatment results with autologous non-cultured cells, support rapid, uncomplicated re-epithelialization with aesthetically and functionally satisfying outcomes. Hospital stays averaged 7.6±1.6 days. Early autologous cell-spray grafting does not preclude or prevent simultaneous or subsequent traditional mesh autografting when indicated on defined areas of full-thickness injury. Copyright © 2016 Elsevier Ltd and ISBI. All rights reserved.

  1. Preliminary results with non-centrifuged autologous fat graft and percutaneous aponeurotomy for treating Dupuytren's disease.

    Science.gov (United States)

    Tuncel, U; Kurt, A; Gumus, M; Aydogdu, O; Güzel, N; Demir, O

    2017-10-01

    The aim of this study was to describe our experience with treating Dupuytren's disease using needle aponeurotomy and non-centrifuged autologous fat grafting. The study included 17 patients (18 hands). Patients were treated with needle aponeurotomy and non-centrifuged autologous fat graft under general anesthesia. The fat grafts were injected into the surgical area so as to stay in contact with the operated site. An extension splint was used for 1 week postoperatively and the patients received hand therapy for 3 weeks. Before the treatment, the contracture in the proximal interphalangeal and metacarpophalangeal joints was a mean of 45.06 ± 13.44 degrees and 36.56 ± 13.09 degrees, respectively. It was 1.61 ± 1.65 and -0.56 ± 3.78 degrees at 3 months, respectively. The difference between these measurements was statistically significant. The mean follow-up period was 12 months. The results were satisfactory and no complications were observed during the follow-up period. Based on the results of the study, percutaneous aponeurotomy with non-centrifuged autologous fat grafting was found to have significantly beneficial effects in the treatment of Dupuytren's disease. Copyright © 2017 SFCM. Published by Elsevier Masson SAS. All rights reserved.

  2. Establishment of autologous embryonic stem cells derived from preantral follicle culture and oocyte parthenogenesis.

    Science.gov (United States)

    Lee, Seung Tae; Choi, Mun Hwan; Lee, Eun Ju; Gong, Seung Pyo; Jang, Mi; Park, Sang Hyun; Jee, Hyang; Kim, Dae Yong; Han, Jae Yong; Lim, Jeong Mook

    2008-11-01

    To evaluate whether autologous embryonic stem cells can be established without generating clone embryos. Prospective model study. Gamete and stem cell biotechnology laboratory in Seoul National University, Seoul, Korea. F1 hybrid B6D2F1 mice. Preantral follicles were cultured, and oocytes matured in the follicles were parthenogenetically activated. Preimplantation development and stem cell characterization. More intrafollicular oocytes that were retrieved from secondary follicles matured and developed into blastocysts after parthenogenesis than those that were retrieved from primary follicles. Of those 35 blastocysts derived from 193 parthenotes, one line of colony-forming cells was established from the culturing of early secondary follicles. The established cells were positive for embryonic stem cell-specific markers and had normal diploid karyotype and telomerase activity. They differentiated into embryoid bodies in vitro and teratomas in vivo. Inducible differentiation of the established cells into neuronal lineage cells also was possible. Autologous embryonic stem cells can be established by preantral follicle culture and oocyte parthenogenesis. A combined technique of follicle culture and oocyte parthenogenesis that does not use developmentally competent oocytes has the potential to replace somatic cell nuclear transfer for autologous cell therapy.

  3. Evolution of Autologous Chondrocyte Repair and Comparison to Other Cartilage Repair Techniques

    Directory of Open Access Journals (Sweden)

    Ashvin K. Dewan

    2014-01-01

    Full Text Available Articular cartilage defects have been addressed using microfracture, abrasion chondroplasty, or osteochondral grafting, but these strategies do not generate tissue that adequately recapitulates native cartilage. During the past 25 years, promising new strategies using assorted scaffolds and cell sources to induce chondrocyte expansion have emerged. We reviewed the evolution of autologous chondrocyte implantation and compared it to other cartilage repair techniques. Methods. We searched PubMed from 1949 to 2014 for the keywords “autologous chondrocyte implantation” (ACI and “cartilage repair” in clinical trials, meta-analyses, and review articles. We analyzed these articles, their bibliographies, our experience, and cartilage regeneration textbooks. Results. Microfracture, abrasion chondroplasty, osteochondral grafting, ACI, and autologous matrix-induced chondrogenesis are distinguishable by cell source (including chondrocytes and stem cells and associated scaffolds (natural or synthetic, hydrogels or membranes. ACI seems to be as good as, if not better than, microfracture for repairing large chondral defects in a young patient’s knee as evaluated by multiple clinical indices and the quality of regenerated tissue. Conclusion. Although there is not enough evidence to determine the best repair technique, ACI is the most established cell-based treatment for full-thickness chondral defects in young patients.

  4. Alveolar ridge preservation with autologous particulated dentin-a case series.

    Science.gov (United States)

    Valdec, Silvio; Pasic, Pavla; Soltermann, Alex; Thoma, Daniel; Stadlinger, Bernd; Rücker, Martin

    2017-12-01

    Ridge preservation can be performed with autologous bone, alloplastic bone substitute material or a combination of both. Dentin is similar to bone in its chemical composition. In its use as bone substitute material, it undergoes a remodelling process and transforms to bone. The presented case report introduces a technique in which the extraction socket is augmented with autologous, particulated dentin. The fractured, non-savable mesial incisor of the upper jaw was carefully extracted in axial direction. After the extraction, the tooth was cleared from remaining periodontal tissue. The vital pulp tissue or a root canal filling, enamel and cementum were also removed. Following the particulation of the remaining dentin in a bone mill, the dentin particles were immediately filled orthotope into the alveolar socket. The soft tissue closure was performed with a free gingival graft of the palate. After an observation period of 4 months, an implant was placed in the augmented area, which osseointegrated successfully and could be restored prosthodontically in the following. The results of this method showed a functional and aesthetic success. The pre-implantological, autologous ridge preservation with dentin could be performed successfully. For the establishment of dentin as augmentation material for jaw augmentation procedures, a prospective, clinical trial is now necessary.

  5. Esterified hyaluronic acid and autologous bone in the surgical correction of the infra-bone defects.

    Science.gov (United States)

    Ballini, Andrea; Cantore, Stefania; Capodiferro, Saverio; Grassi, Felice Roberto

    2009-01-01

    We study the osteoinductive effect of the hyaluronic acid (HA) by using an esterified low-molecular HA preparation (EHA) as a coadjuvant in the grafting processes to produce bone-like tissue in the presence of employing autologous bone obtained from intra-oral sites, to treat infra-bone defects without covering membrane. We report on 9 patients with periodontal defects treated by EHA and autologous grafting (4 males and 5 females, all non smokers, with a mean age of 43.8 years for females, 40.0 years for males and 42 years for all the group, in good health) with a mean depth of 8.3 mm of the infra-bone defects, as revealed by intra-operative probes. Data were obtained at baseline before treatment and after 10 days, and subsequently at 6, 9, and 24 months after treatment. Clinical results showed a mean gain hi clinical attachment (gCAL) of 2.6mm of the treated sites, confirmed by radiographic evaluation. Such results suggest that autologous bone combined with EHA seems to have good capabilities in accelerating new bone formation in the infra-bone defects.

  6. [Treatment of focal bone defect in postoperative nonunion with autologous red bone marrow injection].

    Science.gov (United States)

    Tang, Zhao-hui; Zhu, Li-xing; Xu, Tu-bing; Wang, Kai; Zhou, Xin-min; Li, Qiang

    2009-07-01

    To observe the clinical effect of autologous red bone marrow injection in treating focal bone defect in postoperative nonunion. Thirteen patients with focal bone defect in postoperative nonunion (7 cases in tibia, 2 cases in femur, 4 cases in humerus), including 8 males and 5 females with the mean age of 32.5-years-old (ranging from 15 to 60 years). The bone defects were treated with autologous red bone marrow injection (1 time per 2 weeks, 5 times in total) and the X-rays of AP and LP were observed. Thirteen patients were followed up from 6 to 12 months with an average of 7.5 months. According to results of X-ray pictures, 13 cases obtained bone defect recovered completely, and the average time of union was 4 months. Autologous red bone marrow injection has ascendancy such as less wound and clear clinical effect, which can accelerate bone healing and promotes functional recovery of limb. It is a good method to treat focal bone defect in postoperative nonunion.

  7. Autologous conjunctiva transplantation with stem cells on edge of cornea for recurrent pterygium

    Directory of Open Access Journals (Sweden)

    Yun Wang

    2013-10-01

    Full Text Available AIM: To observe the clinical effectiveness and practicality the autologous conjunctiva transplantation with stem cells on edge of cornea for recurrent pterygium.METHODS: Of the 53 recurrent pterygium patients(57 eyes, after all pathological tissues were removed, underwent the autologous conjunctiva transplantation with stem cells on edge of cornea which were locked above conjunctival transplantation of the operated eye.RESULTS: Postopretive follow-up was 1-12 months for all 57 eyes, of which 3 eyes(5%relapsed. The corneoscleral autolysis was occurred in one eye and surgery treatment was conducted. Corneal wounds were healing and transplantations survived well for the remaining 53 patients without obvious surgical marks. Cure rate was 93%.CONCLUSION: Autologous conjunctiva transplantation with stem cells on edge of cornea for recurrent pterygium can meet the aesthetic requirements of the some patients, with the advantages of obtaining material easily, faster wound healing, lower postoperative recurrence rate, meeting the aesthetic needs of some patients and improving postoperative results. Thus, it is an ideal surgery and is worthy of applying on primary hospital.

  8. An overview on autologous fibrin glue in bone tissue engineering of maxillofacial surgery

    Directory of Open Access Journals (Sweden)

    Azizollah Khodakaram-Tafti

    2017-01-01

    Full Text Available The purpose of this review is to have an overview on the applications on the autologous fibrin glue as a bone graft substitute in maxillofacial injuries and defects. A search was conducted using the databases such as Medline or PubMed and Google Scholar for articles from 1985 to 2016. The criteria were “Autograft,” “Fibrin tissue adhesive,” “Tissue engineering,” “Maxillofacial injury,” and “Regenerative medicine.” Bone tissue engineering is a new promising approach for bone defect reconstruction. In this technique, cells are combined with three-dimensional scaffolds to provide a tissue-like structure to replace lost parts of the tissue. Fibrin as a natural scaffold, because of its biocompatibility and biodegradability, and the initial stability of the grafted stem cells is introduced as an excellent scaffold for tissue engineering. It promotes cell migration, proliferation, and matrix making through acceleration in angiogenesis. Growth factors in fibrin glue can stimulate and promote tissue repair. Autologous fibrin scaffolds are excellent candidates for tissue engineering so that they can be produced faster, cheaper, and in larger quantities. In addition, they are easy to use and the probability of viral or prion transmission may be decreased. Therefore, autologous fibrin glue appears to be promising scaffold in regenerative maxillofacial surgery.

  9. Intralymphatic Immunotherapy With Autologous Semen in a Korean Man With Post-Orgasmic Illness Syndrome.

    Science.gov (United States)

    Kim, Tae Beom; Shim, Young Sup; Lee, Sang Min; Son, Eun Suk; Shim, Jung Woo; Lee, Sang Pyo

    2018-03-14

    Post-orgasmic illness syndrome (POIS) is a very rare disease characterized by local allergic symptoms and transient flu-like illness that nearly always occur after masturbation, coitus, or spontaneous ejaculation and last for 2 to 7 days. In a previous case report, 2 patients with POIS received hyposensitization therapy composed of multiple subcutaneous injections of autologous semen that resulted in a gradual decrease of symptoms. However, this procedure requires patients to endure pain and discomfort during frequent subcutaneous injections and preceding masturbations to obtain the autologous semen used for therapy. Recent studies have suggested that intralymphatic immunotherapy is a promising new method of allergen-specific immunotherapy against allergic diseases, showing a faster onset and longer duration of therapeutic effects after only several intralymphatic injections. We report on a case of a Korean man with POIS who received intralymphatic immunotherapy that alleviated POIS-related symptoms and in whom the existence of semen-specific immunoglobulin E was confirmed using immunoglobulin E immunoblotting and enzyme-linked immunosorbent assay. Kim TB, Shim YS, Lee, SM, et al. Intralymphatic Immunotherapy With Autologous Semen in a Korean Man With Post-Orgasmic Illness Syndrome. Sex Med 2018;X:XX-XX. Copyright © 2018. Published by Elsevier Inc.

  10. Treatment of therapy-refractive ulcera cruris of various origins with autologous keratinocytes in fibrin sealant.

    Science.gov (United States)

    Johnsen, S; Ermuth, T; Tanczos, E; Bannasch, H; Horch, R E; Zschocke, I; Peschen, M; Schöpf, E; Vanscheidt, W; Augustin, M

    2005-02-01

    Evaluation of the effects of cultivated, subconfluent, autologous keratinocytes in fibrin sealant (BioSeed-S) on the healing of therapy-refractive chronic wounds. Open observational study in 60 patients with chronic leg ulcers and impaired wound healing of various origins. After whole-skin excision and cultivation of the autologous keratinocytes, the suspended cells were applied to the preconditioned wound in fibrin sealant. Wound epithelization and wound size were recorded at defined times. Fifty-two of the 60 participating patients could be evaluated. After 6 weeks, 29 ulcers (55.8%) were healed. The mean epithelization increased between the 8th and 42nd postoperative day from 23% to 62.5%. In 50.0% of the patients, global assessment of the wound showed a high degree of epithelization or healing after 42 days. In 32.6% of treated patients, improvement was observed, while no healing tendency was to be found in 17.4%. The present observational study indicates that the transplantation of autologous keratinocytes suspended in fibrin sealant could be of advantage in the treatment of refractive leg ulcers.

  11. Prenatally engineered autologous amniotic fluid stem cell-based heart valves in the fetal circulation.

    Science.gov (United States)

    Weber, Benedikt; Emmert, Maximilian Y; Behr, Luc; Schoenauer, Roman; Brokopp, Chad; Drögemüller, Cord; Modregger, Peter; Stampanoni, Marco; Vats, Divya; Rudin, Markus; Bürzle, Wilfried; Farine, Marc; Mazza, Edoardo; Frauenfelder, Thomas; Zannettino, Andrew C; Zünd, Gregor; Kretschmar, Oliver; Falk, Volkmar; Hoerstrup, Simon P

    2012-06-01

    Prenatal heart valve interventions aiming at the early and systematic correction of congenital cardiac malformations represent a promising treatment option in maternal-fetal care. However, definite fetal valve replacements require growing implants adaptive to fetal and postnatal development. The presented study investigates the fetal implantation of prenatally engineered living autologous cell-based heart valves. Autologous amniotic fluid cells (AFCs) were isolated from pregnant sheep between 122 and 128 days of gestation via transuterine sonographic sampling. Stented trileaflet heart valves were fabricated from biodegradable PGA-P4HB composite matrices (n = 9) and seeded with AFCs in vitro. Within the same intervention, tissue engineered heart valves (TEHVs) and unseeded controls were implanted orthotopically into the pulmonary position using an in-utero closed-heart hybrid approach. The transapical valve deployments were successful in all animals with acute survival of 77.8% of fetuses. TEHV in-vivo functionality was assessed using echocardiography as well as angiography. Fetuses were harvested up to 1 week after implantation representing a birth-relevant gestational age. TEHVs showed in vivo functionality with intact valvular integrity and absence of thrombus formation. The presented approach may serve as an experimental basis for future human prenatal cardiac interventions using fully biodegradable autologous cell-based living materials. Copyright © 2012 Elsevier Ltd. All rights reserved.

  12. Autologous fat injection combined with palatoplasty and pharyngoplasty for velopharyngeal insufficiency and cleft palate: preliminary experience.

    Science.gov (United States)

    Cao, Yimei; Ma, Tingting; Wu, Di; Yin, Ningbei; Zhao, Zhenmin

    2013-08-01

    The aim was to evaluate clinical application of autologous fat transplantation in the posterior pharynx to treat velopharyngeal incompetence and cleft palate. Case series with chart review. Cleft Lip and Palate Center of Plastic Surgery Hospital, an academic medical center. We studied 11 patients (age, 5-26 years) with a cleft palate and velopharyngeal insufficiency who underwent autologous fat injection. Patients were followed for 9 to 40 months. Pronunciation evaluation, visual appearance of the palatopharyngeal area, nasopharyngeal fibroscopy (NPF), palatopharyngeal lateral radiography, and magnetic resonance imaging (MRI) were undertaken before and after the operation. Speech intelligibility was markedly increased in all patients. Pronunciation was good to excellent compared with the preoperative level (P = .001). Mean velopharyngeal insufficiency rate was significantly reduced from 26.05% to 6.96% (P = .028) by NPF and from 26.42% to 7.11% (P = .017) by MRI (axial plane). Magnetic resonance imaging indicated significantly reduced mean minimum velopharyngeal distance, from 10.39 to 3.65 mm (P = .012) in the sagittal plane, and markedly increased thickness of transplanted fat in the posterior pharyngeal wall (sagittal, 5.43 mm; axial, 4.74 mm). There were few complications (sleep apnea, nasopharyngeal regurgitation). Autologous fat transplantation in the posterior pharyngeal wall was a good method for treating velopharyngeal incompetence. The safety profile was good in our sample, and we got a consistent result in the follow-up period. In addition, it also could be combined with routine surgery.

  13. IMMUNE STATE IN PATIENTS WITH HEMATOLOGICAL MALIGNANCIES AT LATE TERMS AFTER AUTOLOGOUS HEMATOPOIETIC STEM CELL TRANSPLANTATION

    Directory of Open Access Journals (Sweden)

    N. V. Minaeva

    2012-01-01

    Full Text Available Abstract. Autologous hematopoietic stem cell transplantation (auto-HSCT is one of the most effective methods for treatment of patients with various forms of hemoblastoses, both in adults and children. However, high-dose chemotherapy protocols used in this procedure are characterized by pronounced myeloand immunotoxicity. Appropriate data concerning immune state at long terms after high-dose chemotherapy and auto-HSCT are sparse and controversial, and there is no consensus on time dynamics of immune system reconstitution. The aim of this study was a comprehensive evaluation of immunity in recipients of auto-HSCT at longer terms. Clinical and immunological testing was performed in ninety-eight patients with hematological malignancies before starting a high-dose chemotherapy, and at late post-transplant period. The state of cellular immunity was assessed as expression of surface CD3+, CD4+, CD8+, CD16+, CD19+ lymphocyte antigens. Humoral immunity was evaluated by serum IgG, IgA, and IgM levels. The studies have revealed disorders of cellular and humoral immunity, as well as nonspecific immune resistance factors in recipients of autologous hematopoietic stem cells at late terms post-transplant. Immune reconstitution in patients receiving highdose consolidation treatment followed by auto-HSCT takes longer time than in patients who did not receive autologous hematopoietic stem cells. Severity of these disturbances and immune reconstitution rates depend on the type of conditioning regimen, and the source of haematopoietic stem cells used for transplantation.

  14. Effect of acetylsalicylic acid on microvascular thrombosis in autologous breast reconstruction.

    Science.gov (United States)

    Enajat, Morteza; Aziz Mohammadi, Mujzgan; Debeij, Jan; van der Hulst, Rene R W J; Mureau, Marc A M

    2014-01-01

    Although advances in microsurgery have increased success rates of autologous breast reconstruction, microvascular thrombosis still remains a major concern as a cause of flap failure. At present, no evidence-based guidelines on pharmacological prevention of microvascular thrombosis exist. This study investigates the effect of acetylsalicylic acid on the incidence of microvascular complications in patients undergoing autologous breast reconstruction. Patients undergoing deep inferior epigastric artery perforator or free transverse rectus abdominis myocutaneous flap breast reconstruction at two academic centers in the Netherlands between 2005 and 2011 were included. Patients at one center received once daily 0.6 mL of nadroparine and 40 mg acetylsalicylic acid, while patients at the other center received 0.6 mL nadroparine only. A total of 430 consecutive patients underwent 592 breast reconstructions. No statistically significant differences were found between the two groups in the incidence of flap failure (2.8 and 2.5%), microvascular thromboembolic complications (2.6 and 3.8%), venous congestion (3.4 and 2.8%), or overall complications (28.0 and 32.3%). Hematoma tended to occur more often in the group receiving acetylsalicylic acid (9.2 and 4.7%).It was found that no protective effect of acetylsalicylic acid on microvascular complications was present. Given its known risks and the somewhat increased occurrence of hematoma in the present study, we stopped to routinely administer acetylsalicylic acid after autologous breast reconstruction. Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

  15. Magnetic resonance tomography-guided interventional procedure for diagnosis of prostate cancer; MRT-gezielte interventionelle Verfahren zur Abklaerung des Prostatakarzinoms

    Energy Technology Data Exchange (ETDEWEB)

    Schernthaner, M.; Helbich, T.H.; Fueger, B.J.; Memarsadeghi, M.; Stiglbauer, A.; Linhart, H.G.; Doan, A.; Pinker, K.; Brader, P. [Medizinische Universitaet Wien, Univ.-Klinik fuer Radiodiagnostik, Division fuer Molekulare und Gender-Bildgebung, Wien (Austria); Margreiter, M. [Medizinische Universitaet Wien, Univ.-Klinik fuer Urologie, Wien (Austria)

    2011-11-15

    In recent years magnetic resonance imaging (MRI) has been increasingly established in the diagnosis of prostate cancer in addition to transrectal ultrasonography (TRUS). The use of T2-weighted imaging allows an exact delineation of the zonal anatomy of the prostate and its surrounding structures. Other MR imaging tools, such as dynamic contrast-enhanced T1-weighted imaging or diffusion-weighted imaging allow an inference of the biochemical characteristics (multiparametric MRI). Prostate cancer, which could only be diagnosed using MR imaging or lesions suspected as being prostate cancer, which are localized in the anterior aspect of the prostate and were missed with repetitive TRUS biopsy, need to undergo MR guided biopsy. Recent studies have shown a good correlation between MR imaging and histopathology of specimens collected by MR-guided biopsy. Improved lesion targeting is therefore possible with MR-guided biopsy. So far data suggest that MR-guided biopsy of the prostate is a promising alternative diagnostic tool to TRUS-guided biopsy. (orig.) [German] Neben dem transrektalen Ultraschall (TRUS) hat sich in den letzten Jahren die MRT als nichtinvasive Methode zur Bildgebung von Prostatatumoren etabliert. Mittels T2-gewichteter Sequenzen ist eine exakte anatomische Darstellung der Prostata und ihrer umliegenden Strukturen moeglich. Andere MRT-Techniken ermoeglichen Rueckschluesse auf das biologische Verhalten des Tumors: dynamische kontrastmittelverstaerkte T1-gewichtete Sequenzen zur Darstellung der Angiogenese, diffusionsgewichtete Aufnahmen zur Beurteilung der Zelldichte und die Spektroskopie zur Bestimmung von Gewebemetaboliten wie Cholin und Kreatin (multiparametrische Bildgebung). Prostatatumoren, die nur mittels MRT nachweisbar sind oder verdaechtige Tumoren, die hauptsaechlich anterior in der Prostata lokalisiert sind und in wiederholten TRUS-gezielten Biopsien verfehlt wurden, benoetigen eine MRT-gezielte Biopsie zur Diagnosesicherung. Die bisherigen

  16. Entwicklung vereinfachter flüssigchromatographischer 
Untersuchungsmethoden zur Qualitätskontrolle essentieller Antimalaria-Medikamente in Entwicklungs- und 
Schwellenländern

    OpenAIRE

    Höllein, Ludwig

    2015-01-01

    Im Rahmen dieser Arbeit wurden sehr einfache, flüssigchromatographische Methoden zur Qualitätsanalytik gebräuchlicher Antimalaria-Medikamente (Amodiaquin, Mefloquin, Proguanil sowie die Kombination Artemether/Lumefantrin) entwickelt, die nur wenige, günstig erhältliche Chemikalien (Phosphatpuffer, Methanol) sowie gewöhnliche, kommerzielle RP-18-Säulen benötigen. Sie sind insbesondere zur Anwendung in Laboratorien in Entwicklungsländern geeignet und erfordern keine komplexen HPLC-Instrumente w...

  17. A Nationwide Analysis of Cost Variation for Autologous Free Flap Breast Reconstruction.

    Science.gov (United States)

    Billig, Jessica I; Lu, Yiwen; Momoh, Adeyiza O; Chung, Kevin C

    2017-11-01

    Cost variation among hospitals has been demonstrated for surgical procedures. Uncovering these differences has helped guide measures taken to reduce health care spending. To date, the fiscal consequence of hospital variation for autologous free flap breast reconstruction is unknown. To investigate factors that influence cost variation for autologous free flap breast reconstruction. A secondary cross-sectional analysis was performed using the Healthcare Cost and Utilization Project National Inpatient Sample database from 2008 to 2010. The dates of analysis were September 2016 to February 2017. The setting was a stratified sample of all US community hospitals. Participants were female patients who were diagnosed as having breast cancer or were at high risk for breast cancer and underwent autologous free flap breast reconstruction. Variables of interest included demographic data, hospital characteristics, length of stay, complications (surgical and systemic), and inpatient cost. The study used univariate and generalized linear mixed models to examine associations between patient and hospital characteristics and cost. A total of 3302 patients were included in the study, with a median age of 50 years (interquartile range, 44-57 years). The mean cost for autologous free flap breast reconstruction was $22 677 (interquartile range, $14 907-$33 391). Flap reconstructions performed at high-volume hospitals were significantly more costly than those performed at low-volume hospitals ($24 360 vs $18 918, P cost (Exp[β], 1.06; 95% CI, 1.02-1.11; P = .003). Fewer surgical complications (16.4% [169 of 1029] vs 23.7% [278 of 1174], P cost variation among patients undergoing autologous free flap breast reconstruction. Experience, as measured by a hospital's volume, provides quality health care with fewer complications but is more costly. Longer length of stay contributed to regional cost variation and may be a target for decreasing expenditure, without

  18. Evaluation of ex vivo produced endothelial progenitor cells for autologous transplantation in primates.

    Science.gov (United States)

    Qin, Meng; Guan, Xin; Zhang, Yu; Shen, Bin; Liu, Fang; Zhang, Qingyu; Ma, Yupo; Jiang, Yongping

    2018-01-22

    Autologous transplantation of endothelial progenitor cells (EPCs) is a promising therapeutic approach in the treatment of various vascular diseases. We previously reported a two-step culture system for scalable generation of human EPCs derived from cord blood CD34 + cells ex vivo. Here, we now apply this culture system to expand and differentiate human and nonhuman primate EPCs from mobilized peripheral blood (PB) CD34 + cells for the therapeutic potential of autologous transplantation. The human and nonhuman primate EPCs from mobilized PB CD34 + cells were cultured according to our previously reported system. The generated adherent cells were then characterized by the morphology, surface markers, nitric oxide (NO)/endothelial NO synthase (eNOS) levels and Dil-acetylated low-density lipoprotein (Dil-Ac-LDL) uptake/fluorescein isothiocyanate (FITC)-lectin binding actives. Furthermore, the efficacy and safety studies were performed by autologous transplantation via hepatic portal vein injection in a nonhuman primate model with acute liver sinusoidal endothelial cell injury. The mobilized PB CD34 + cells from both human and nonhuman primate were efficiently expanded and differentiated. Over 2 × 10 8 adherent cells were generated from 20 mL mobilized primate PB (1.51 × 10 6  ± 3.39 × 10 5 CD34 + cells) by 36-day culture and more than 80% of the produced cells were identified as EPCs/endothelial cells (ECs). In the autologous transplant model, the injected EPC/ECs from nonhuman primate PB were scattered in the intercellular spaces of hepatocytes at the hepatic tissues 14 days post-transplantation, indicating successful migration and reconstitution in the liver structure as the functional EPCs/ECs. We successfully applied our previous two-step culture system for the generation of primate EPCs from mobilized PB CD34 + cells, evaluated the phenotypes ex vivo, and transplanted autologous EPCs/ECs in a nonhuman primate model. Our study indicates that

  19. Gene editing of CCR5 in autologous CD4 T cells of persons infected with HIV.

    Science.gov (United States)

    Tebas, Pablo; Stein, David; Tang, Winson W; Frank, Ian; Wang, Shelley Q; Lee, Gary; Spratt, S Kaye; Surosky, Richard T; Giedlin, Martin A; Nichol, Geoff; Holmes, Michael C; Gregory, Philip D; Ando, Dale G; Kalos, Michael; Collman, Ronald G; Binder-Scholl, Gwendolyn; Plesa, Gabriela; Hwang, Wei-Ting; Levine, Bruce L; June, Carl H

    2014-03-06

    CCR5 is the major coreceptor for human immunodeficiency virus (HIV). We investigated whether site-specific modification of the gene ("gene editing")--in this case, the infusion of autologous CD4 T cells in which the CCR5 gene was rendered permanently dysfunctional by a zinc-finger nuclease (ZFN)--is safe. We enrolled 12 patients in an open-label, nonrandomized, uncontrolled study of a single dose of ZFN-modified autologous CD4 T cells. The patients had chronic aviremic HIV infection while they were receiving highly active antiretroviral therapy. Six of them underwent an interruption in antiretroviral treatment 4 weeks after the infusion of 10 billion autologous CD4 T cells, 11 to 28% of which were genetically modified with the ZFN. The primary outcome was safety as assessed by treatment-related adverse events. Secondary outcomes included measures of immune reconstitution and HIV resistance. One serious adverse event was associated with infusion of the ZFN-modified autologous CD4 T cells and was attributed to a transfusion reaction. The median CD4 T-cell count was 1517 per cubic millimeter at week 1, a significant increase from the preinfusion count of 448 per cubic millimeter (PCCR5-modified CD4 T cells at 1 week was 250 cells per cubic millimeter. This constituted 8.8% of circulating peripheral-blood mononuclear cells and 13.9% of circulating CD4 T cells. Modified cells had an estimated mean half-life of 48 weeks. During treatment interruption and the resultant viremia, the decline in circulating CCR5-modified cells (-1.81 cells per day) was significantly less than the decline in unmodified cells (-7.25 cells per day) (P=0.02). HIV RNA became undetectable in one of four patients who could be evaluated. The blood level of HIV DNA decreased in most patients. CCR5-modified autologous CD4 T-cell infusions are safe within the limits of this study. (Funded by the National Institute of Allergy and Infectious Diseases and others; ClinicalTrials.gov number, NCT00842634.).

  20. Efficacy of autologous conjunctival flap on repairing the late-onset filtering bleb leakage

    Directory of Open Access Journals (Sweden)

    Jian-Gang Xu

    2018-04-01

    Full Text Available AIM: To evaluate the effectiveness of autologous conjunctival flap surgery for repairing the late-onset filtering bleb leakage after trabeculectomy. METHODS: This study retrospectively reviewed 106 eyes from 106 patients with late-onset filtering bleb leakage after trabeculectomy who received autologous conjunctival flap surgery at the Zhongshan Ophthalmic Centre from 2005 to 2015. The basic information was recorded, and the interval time between trabeculectomy and autologous conjunctival flap surgery as well as related risk factors, intraocular pressure (IOP, anterior chamber depth (ACD and best corrected visual acuity (BCVA were analysed. Moreover, 41 patients who completed the 1-year follow up were analysed to determine the IOP and BCVA changes and long-term success rates. RESULTS: The 50 male and 56 female subjects (average age 39.13±17.96y included 47 (44.34% and 33 (31.13% cases of primary open angle and primary angle-closure glaucoma. The mean interval between trabeculectomy and repair surgery was 60.60±56.92 (3-264mo. The mean mitomycin (MMC concentration during trabeculectomy was 0.27±0.04 (0.12-0.4 mg/mL in the fornix-based conjunctival flap group (68 patients and 0.28±0.04 (0.20-0.33 mg/mL in the limbal-based conjunctival flap group (11 patients. After bleb leakage, the patients' vision remained stable while the IOP decreased from 10.25±4.76 (3-20.86 to 9.44±4.33 (2-21 mm Hg (P<0.01. In the 41 analysed patients, the IOP was controlled at 15.68±5.11 (7-40 mm Hg in the 1st year after autologous conjunctival flap surgery and recurrence was not observed, for a long-term success rate of 100%. CONCLUSION: Autologous conjunctival flap repairing surgery is an effective technique for sealing filtering bleb leakages and controlling IOP postoperatively.

  1. Effect of Platelet-Rich Plasma (PRP) versus Autologous Whole Blood on Pain and Function Improvement in Tennis Elbow: A Randomized Clinical Trial

    OpenAIRE

    Raeissadat, Seyed Ahmad; Sedighipour, Leyla; Rayegani, Seyed Mansoor; Bahrami, Mohammad Hasan; Bayat, Masume; Rahimi, Rosa

    2014-01-01

    Background. Autologous whole blood and platelet-rich plasma (PRP) have been both suggested to treat chronic tennis elbow. The aim of the present study was to compare the effects of PRP versus autologous whole blood local injection in chronic tennis elbow. Methods. Forty patients with tennis elbow were randomly divided into 2 groups. Group 1 was treated with a single injection of 2 mL of autologous PRP and group 2 with 2 mL of autologous blood. Tennis elbow strap, stretching, and strengthening...

  2. Early resumption of physical activities leads to inferior clinical outcomes after matrix-based autologous chondrocyte implantation in the knee.

    Science.gov (United States)

    Niethammer, Thomas R; Müller, Peter E; Safi, Elem; Ficklscherer, Andreas; Roßbach, Björn P; Jansson, Volkmar; Pietschmann, Matthias F

    2014-06-01

    Matrix-based autologous chondrocyte implantation is a well-established operation procedure for full cartilage defects. When to resume physical activity after matrix-based autologous chondrocyte implantation is controversial. Our hypothesis was that early resumption of physical activity leads to a worse clinical outcome after matrix-based autologous chondrocyte implantation in the knee two years post-operatively. Physical activity is defined as any kind of impact sport. Forty-four patients with cartilage defects of the knee were treated with matrix-based autologous chondrocyte implantation (Novocart3D). All patients were assessed preoperatively and after a period of 24 months with the University of California Los Angeles (UCLA) Activity score. The return to physical activities or sports after matrix-based autologous chondrocyte implantation was documented. Patients were evaluated using the International Knee Documentation Committee Knee Examination Form and visual analogue scale for pain after 6, 12 and 24 months. Fifty-five percent showed an unchanged level of physical activity in the UCLA Activity score post-operatively. About 35% showed a lower level and 10% a higher level of physical activity. The average return to physical activities or sports after matrix-based autologous chondrocyte implantation procedure was 10.2 months. Patients with a later return of sports after 12 months showed significantly better clinical results after two years. In particular, patients who started practicing impact sport after 12 months post-operatively showed significantly better results. Resuming physical activity including impact sports without waiting at least 12 months after the operation leads to inferior outcomes up to 24 months after matrix-based autologous chondrocyte implantation. Level IV.

  3. Comparison of autologous versus allogeneic epithelial-like stem cell treatment in an in vivo equine skin wound model.

    Science.gov (United States)

    Broeckx, Sarah Y; Borena, Bizunesh M; Van Hecke, Lore; Chiers, Koen; Maes, Sofie; Guest, Deborah J; Meyer, Evelyne; Duchateau, Luc; Martens, Ann; Spaas, Jan H

    2015-10-01

    Several studies report beneficial effects of autologous and allogeneic stem cells on wound healing. However, no comparison between autologous versus allogeneic epithelial-like stem cells (EpSCs) has been made so far. For this reason, we first hypothesize that both EpSC types enhance wound healing in comparison to vehicle treatment and untreated controls. Second, on the basis of other studies, we hypothesized that there would be no difference between autologous and allogeneic EpSCs. Twelve full-thickness skin wounds were created in six horses. Each horse was subjected to (i) autologous EpSCs, (ii) allogeneic EpSCs, (iii) vehicle treatment or (iv) untreated control. Wound evaluation was performed at day 3, 7 and 14 through wound exudates and at week 1, 2 and 5 through biopsies. Wound circumference and surface were significantly smaller in autologous EpSC-treated wounds. A significantly lower amount of total granulation tissue (overall) and higher vascularization (week 1) was observed after both EpSC treatments. Significantly more major histocompatibility complex II-positive and CD20-positive cells were noticed in EpSC-treated wounds at week 2. In autologous and allogeneic groups, the number of EpSCs in center biopsies was low after 1 week (11.7% and 6.1%), decreased to 7.6% and 1.7%, respectively (week 2), and became undetectable at week 5. These results confirm the first hypothesis and partially support the second hypothesis. Besides macroscopic improvements, both autologous and allogeneic EpSCs had similar effects on granulation tissue formation, vascularization and early cellular immune response. Copyright © 2015 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

  4. Carrying out a program for the support of photoelectric compact systems; 3. ed.; Durchfuehrung eines Programmes zur Foerderung photovoltaischer Kompaktsysteme

    Energy Technology Data Exchange (ETDEWEB)

    Bendel, C.

    1996-08-01

    The work target of the project consists of solving scientific/technical problems in the field of system technique of photoelectric compact systems of small output: - Reducing the manufacturing costs - Improving the efficiency - Improving the transfer of know-how from Research Institutions to industry - Supporting small and medium sized firms - Supporting the trade by improved transfer of know-how. (HW) [Deutsch] Das Arbeitsziel des Vorhabens bestand in der Loesung wissenschaftlich-technischer Probleme im Bereich der Systemtechnik photovoltaischer Kompaktsysteme kleiner Leistung: - Reduzierung der Herstellungskosten - Wirkungsgradverbesserung - Verbesserung des Know-How-Transfers vom Forschungsinstitut zur Industrie - Foerderung von Klein- u. mittelstaendischen Unternehmen - Unterstuetzung des Handwerks durch verbesserten Know-How Transfer. (HW)

  5. Role of imaging procedures in clarification of complications of pneumonia; Rolle der bildgebenden Verfahren zur Abklaerung von Pneumoniekomplikationen

    Energy Technology Data Exchange (ETDEWEB)

    Lampichler, K. [Medizinische Universitaet Wien, Universitaetsklinik fuer Radiologie und Nuklearmedizin, Allgemeines Krankenhaus, Wien (Austria)

    2017-01-15

    Despite a considerable number of antimicrobial agents and interdisciplinary treatment options, lower respiratory tract infections are still associated with high morbidity and mortality rates. Infections of the respiratory tract can lead to severe complications, such as empyema, lung abscesses and acute respiratory distress syndrome (ARDS). Besides intrapulmonary complications pneumonia can also impair other organs due to a systemic inflammatory response. Underlying cardiovascular diseases, such as chronic heart failure, arteriosclerosis and dysrhythmia can either deteriorate due to infections or be newly manifested as a result of pneumonia. Early diagnosis and therapy of these sometimes life-threatening complications are crucial and can have a severe impact on disease outcome. The most important imaging techniques include chest X-ray and computed tomography (CT) of the chest. Although a definite diagnosis is sometimes difficult or even impossible to establish using chest X-ray or CT, there are several findings indicative of intrapulmonary or extrapulmonary complications of pneumonia. Another useful and portable tool is thoracic ultrasound directly on the patient, which can be used to further define the underlying disease or as guidance during procedures. The chest X-ray is useful in initial diagnosis and follow-up. If complications or disease progression are suspected or the clinical course differs from the X-ray interpretation, a subsequent CT of the chest should be performed. (orig.) [German] Pneumonien sind trotz antimikrobieller Therapien und multidisziplinaerer Behandlungsstrategien unveraendert mit einer hohen Morbiditaets- und Mortalitaetsrate verbunden. Eine Infektion des Respirationstrakts kann zu Komplikationen wie Lungenempyem, -abszess und akutem Lungenversagen fuehren. Zusaetzlich kommt es neben den intrapulmonalen Komplikationen haeufig auch zur Beeintraechtigung anderer Organe. Kardiovaskulaere Grunderkrankungen wie Herzinsuffizienz, Arteriosklerose

  6. School Shootings in Deutschland: Aktuelle Trends zur Prävention von schwerer, zielgerichteter Gewalt an deutschen Schulen

    OpenAIRE

    Bondü, Rebecca; Scheithauer, Herbert

    2009-01-01

    Die School Shootings in Winnenden und Ansbach im März und September 2009 haben die Notwendigkeit präventiver Ansätze zur Vermeidung weiterer Taten in Deutschland erneut verdeutlicht. Aufgrund der geringen Häuigkeit des Phänomens und der geringen Speziität der bekannten Risikofaktoren ist eine gezielte Vorhersage und Prävention jedoch schwierig. Dennoch werden zurzeit verschiedene Präventionsansätze diskutiert. Der vorliegende Artikel stellt diese vor und beleuchtet ihre Vor- und Nachteile. Da...

  7. Evaluation der Kurse 'Deutsch als Fremdsprache' im Akademischen Auslandsamt Siegen : ein Beitrag zur Qualitätssicherung und -entwicklung

    OpenAIRE

    Moll, Katharina

    2011-01-01

    Der Begriff "Qualität" im Kontext von Lehre und Unterricht ist schon seit Jahren in aller Munde. Nicht erst, aber insbesondere seit der Veröffentlichung der ersten PISA-Studie fordern Politiker umfassende Managementsysteme zur Verbesserung der Qualität von Bildungseinrichtungen in Deutschland. In diesem Zusammenhang wurden in den letzten Jahren viele Ideen entwickelt, die helfen sollen, die Qualität der Bildung zu verbessern. Vor allem für den schulischen Bereich, der besonders unter Druck ge...

  8. Untersuchungen zur Zytotoxizität von dentalen Füllungsmaterialien am Beispiel von Amalgam und Gallium-alloy

    OpenAIRE

    Ungerer, Sebastian Steffen

    2004-01-01

    Amalgam wird in der Zahnheilkunde schon seit 1819 als Füllungsmaterial eingesetzt. Die prob-lematische Biokompatibilität des Amalgams wurde jedoch nicht oder nur wenig beachtet. Galli-um und Indium wurden in den 1950er Jahren als Ersatz für Quecksilber verwendet und ein Fül-lungswerkstoff, Gallium-alloy, entwickelt, der mit dem konventionellen Amalgam vergleichbar war. Die in vitro Untersuchungen zur Biokompatibilität wurden in den 1990er Jahren in erster Linie an Fibroblasten durchgeführt. D...

  9. Zur Politik der Intertextualität in Roberto Bolaños ‚Estrella Distante‘

    OpenAIRE

    Samir Sellami

    2014-01-01

    Am Beispiel von Bolaños 1996 erschienenem Kurzroman Estrella Distante untersucht der Beitrag die spezifische Logik der Intertextualität in Bolaños Poetik. Seine implizite Logik der Intertextualität entwickelt sich dabei nicht im luftleeren Raum, sondern im Kontrastverhältnis zu der erzählten Poetik des Protagonisten Carlos Wieder. Dessen formal progressive, aber politisch reaktionär motivierte Poetik deckt die fundamentale Ambivalenz avantgardistischer Poetiken auf und führt zur gegensätzl...

  10. Zur Konzeption kritischer Stadtforschung. Ansätze jenseits einer Eigenlogik der Städte

    Directory of Open Access Journals (Sweden)

    Jan Kemper

    2013-06-01

    Full Text Available Kritische Stadtforschung dient in Gestalt der „kritischen Stadtsoziologie“ gegenwärtig als Negativfolie für jene Stadtforschungsperspektive, die mit dem Postulat einer „Eigenlogik der Städte“ (EdS antritt. Eine (Selbst-Verständigung über konzeptionelle Grundlagen und forschungsstrategische Absichten einer kritischen Stadtforschung dagegen gibt es kaum. Ausgehend von dieser Beobachtung ist es das Ziel dieses Beitrags, zu einer Konturierung kritischer Stadtforschung beizutragen. Dafür skizzieren wir zunächst Eckpunkte der EdS-Perspektive. Anschließend wird ein Blick zurück auf den Klassiker Die Lage der arbeitenden Klasse in England (1845 von Friedrich Engels geworfen. Daran verdeutlichen wir drei Schritte in der Etablierung einer kritischen Haltung in der Stadtforschung: die Berücksichtigung der historisch-sozialen Voraussetzungen für gegenwärtige Stadtentwicklungsprozesse, die Analyse der städtischen Raum- und Sozialverhältnisse als krisen- und konfliktvermittelte und deshalb als dynamische Verhältnisse sowie die Konzeptionierung dieser Verhältnisse als veränderbar. An drei wiederkehrenden Diskussionsfeldern sozialwissenschaftlicher Stadtforschung schließlich verfolgen wir, wie einerseits die EdS-Perspektive sich dieser Themen annimmt und wie andererseits eine kritische Haltung im Umgang mit diesen Themen eingenommen werden kann. Für eine kritische Stadtforschung schlagen wir vor, (a „Stadt“ nicht als dauerhaft definiert und nicht als erklärend für sozialräumliche Verhältnisse zu begreifen, (b auch Städte als Räume zu konzipieren, die sich im gegenseitigen Wechselverhältnis mit Gesellschaft konstituieren, (c mit einem Konzept von Differenz, das Unterschiede sozial erklärt, zu arbeiten, wenn Städte als einzigartig erlebte Orte analysiert werden sollen.

  11. Environmental compatibility of chemicals for sewage treatment; Umweltvertraeglichkeit von Chemikalien zur Abwasserbehandlung

    Energy Technology Data Exchange (ETDEWEB)

    Schumann, H.; Obst, K.; Friedrich, C.; Pattard, M.; Pluta, H.J.; Hahn, J.

    1997-05-30

    Due to the use of chemicals for waste water treatment the treated waste water and the effluents are polluted by the accompanying substance matrix of the chemicals. Furthermore, because of overstoichiometric dosage or additives also a not reacting part of toxic substances gets into the treated waste water and effluents. Therefore it is necessary to prevent that through waste water treatment further environmentally incompatible substances get into the waters. Within the framework of a research project promoted by the Federal Ministry of the Environment, Nature Conservation and Reactor Safety proposals were made for guide values and respectively limit values for coagulant and flocculant salts, lime products, neutralising agents, technical hydrochloric acid, technical sulphuric acid, polyacrylamides and organic sulphuric compounds. In contrast to most of the anorganic chemicals for waste water treatment, which show only a relatively low increase of heavy metal concentrations caused by the accompanying substance matrix, organic chemicals for sewage treatment are partly considered to be problematic substances because of an adverse combination of characteristics. (orig.) [Deutsch] Durch den Einsatz von Chemikalien zur Behandlung von Abwaessern gelangen - Verunreinigungen durch die Nebenstoff-Matrix der eingesetzten Behandlungschemikalien in das behandelte Abwasser und in die Gewaesser und - durch ueberstoechiometrische Dosierung oder Additive tritt der nicht reagierende Teil toxischer Substanzen ebenfalls im behandelten Abwasserablauf und im Gewaesser auf. Vor diesem Hintergrund gilt es zu verhindern, dass durch Massnahmen der Abwasserbehandlung zusaetzlich umweltunvertraegliche Stoffe in die Gewaesser eingetragen werden. Im Rahmen eines vom Bundesministerium fuer Umwelt, Naturschutz und Reaktorsicherheit gefoerderten Forschungsvorhabens wurden Vorschlaege fuer Richtwerte bzw. Anforderungen an Faellungs- und Flockungssalze, Kalkprodukte, Natronlauge, Soda, Salzsaeure

  12. Recommendations on procedures for renal function imaging; Anleitung zur Durchfuehrung der Nierenfunktionsszintigraphie

    Energy Technology Data Exchange (ETDEWEB)

    Klaeser, B.; Eberl, A.; Bubeck, B. [Klinik fuer Nuklearmedizin, Kantonsspital St. Gallen (Switzerland)

    2003-09-01

    Renal function diagnostics in nuclear medicine is nowadays performed primarily as scintigraphic study using {sup 99m}Tc-MAG3, in many cases accompanied by quantitative clearance measurement. The present recommendations are based on the technical protocols of the Clinic of Nuclear Medicine at the Kantonsspital St. Gallen and do not claim to be complete or the Gold Standard. The procedures described are not subject to discussion, but the authors attempt to give practical guidelines as orientation for users in their daily routine. Focal points are preparation and performance of the diuretic renography with furosemide to evaluate urinary outflow obstructions and scintigraphic studies under captopril for detection of renovascular hypertension. Further subjects are the quantitative clearance measurement according to the single-sample method and, of course, the calculation of the split clearance. All of these subjects are described mainly under practical aspects, looking in particular at frequent pitfalls or wrong interpretations, but also taking into account quality assurance and data analysis. Selected cases are presented to document the importance of renal function imaging in practice. (orig.) [German] Die nuklearmedizinische Nierenfunktionsdiagnostik wird heute vorrangig als Szintigraphie mit {sup 99m}Tc-MAG3 betrieben, haeufig mit begleitender quantitativer Clearancemessung. Die vorliegende Anleitung basiert auf den Untersuchungsprotokollen der Klinik fuer Nuklearmedizin des Kantonsspitals St. Gallen und erhebt weder Anspruch auf Vollstaendigkeit noch darauf, als Goldstandard zu gelten. Die beschriebenen Vorgehensweisen werden nicht diskutiert, sondern die Autoren versuchen, pragmatische Leitlinien zu vermitteln, an denen sich der Anwender in der taeglichen Routine orientieren kann. Schwerpunkte bilden die Vorbereitung und Durchfuehrung der so genannten Lasix-Szintigraphie zur Beurteilung der renalen Abflussverhaeltnisse und die Captopril-Szintigraphie bei der Frage

  13. Tradable permits. The stony path to a market economy; Umweltzertifikate. Der steinige Weg zur Marktwirtschaft

    Energy Technology Data Exchange (ETDEWEB)

    Bonus, H. [ed.

    1998-12-31

    Thirty years ago, Thomas D. Crocker and John H. Dales came up with the idea of tradable permits for making environmental protection both economically and ecologically efficient. Since then, a heated and controversial discussion has been going on. Tradable permits were considered as unrealistic, contra-competitive, incompatible with political structures, and even as an attempt to treat Mother Nature as a whore. As a result, Germany still operates by the command-and-control principle, and there are no significant examples of emission trading. The first part of this publication reviews the theoretical discussion and practical experiemce so far, while the second part discusses the requirements that must be met by tradable permit systmes in order to make them practicable. [Deutsch] Dreissig Jahre Diskussion ueber Zertifikate zeigen, dass der Weg zu einer marktwirtschaftlich ausgerichteten Umweltpolitik steinig ist. Die bahnbrechende Idee von Thomas D. Crocker und John H. Dales, mit Zertifikaten Umweltschutz sowohl oekonomisch effizient wie auch oekologisch treffsicher zu realisieren, wurde in der Vergangenheit immer wieder von Zweifeln an der Praktikabilitaet einer solchen Loesung ueberlagert. So wurde der Handel mit Zertifikaten als unrealisierbar angesehen, wettbewerbs- und strukturpolitische Einwaende wurden angefuehrt, und man empfand die Vergabe von Rechten zur Umweltnutzung sogar als Prostitution von Mutter Natur. Als Folge hat Deutschland bis heute kein signifikantes Umsetzungsbeispiel vorzuweisen; das Ordnungsrecht beherrscht nach wie vor die Szene. Der erste Teil des Bandes beleuchtet zunaechst den Stand der theoretischen Diskussion und die bisher gemachten Erfahrungen mit Zertifikaten. Der zweite Teil ist den Anforderungen gewidmet, denen Zertifikatesysteme gerecht werden muessen, um Eingang in die Praxis zu finden. (orig.)

  14. LINGUA TERTII IMPERII. VICTOR KLEMPERERS DIARISTISCHE ERÖRTERUNGEN ZUR ANTISEMITISCHEN HASSREDE

    Directory of Open Access Journals (Sweden)

    Arvi SEPP

    2010-11-01

    Full Text Available Victor Klemperers Tagebücher Ich will Zeugnis ablegen bis zum letzten: Tagebücher 1933-1945 haben nach ihrem Erscheinen Mitte der 1990er Jahre ein starkes Echo gefunden und gehören inzwischen zum Kanon der Tagebuchliteratur. Das zweibändige, während der nationalsozialistischen Diktatur entstandene Tagebuchwerk beschreibt die Lebenswelt eines deutsch-jüdischen Verfolgten im Dritten Reich. Der Antisemitismus zwang den Philologen zu einer Auseinandersetzung mit dem Relevanzverlust seiner Assimilationsbemühungen und seiner Kennzeichnung als Jude. Eine rekurrente Kompensationstaktik gegen seine erzwungene Randstellung besteht in der sprachkritischen Demontierung des Rassendenkens, um die Ungültigkeit des Antisemitismus nachzuweisen. Die Beobachtungen zur Lingua Tertii Imperii, die er in den Tagebuchblättern aufschrieb, wurden immer mehr zu seinem eigentlichen Werk. Das Tagebuch ist vor diesem Hintergrund in weiten Teilen zum Arbeitsjournal für die Sprachund Kulturanalysen Klemperers geworden. Der Interdependenz von Sprachkritik und Mentalitätsgeschichte in Klemperers Analyse der Sprache des Dritten Reiches gilt im vorliegenden Aufsatz das Hauptinteresse. Im ersten Teil wird der Frage nachgegangen, auf welche Art und Weise Victor Klemperer in seinen Notizen die Beziehung zwischen Sprache, Ideologie und Mentalität verstand. Im zweiten Teil wird unter Rückgriff auf Judith Butler und Louis Althusser in den Vordergrund gerückt, wie Victor Klemperer im Tagebuch vor Augen führt, dass das verletzende Sprechen gegen Juden im Nationalsozialismus eine subjektkonstituierende Wirkung hatte. Im letzten Teil des Aufsatzes gilt das Hauptaugenmerk der vom Tagebuchautor festgestellten diskursiven Einverleibung antisemitischer Rhetorik durch die verfolgte jüdische Bevölkerung.

  15. Poietik der Ambivalenz. Zur Sinngenerierung in Tizians ‚Vergewaltigung der Lucretia‘ (1571

    Directory of Open Access Journals (Sweden)

    Karin Gludovatz

    2010-11-01

    Full Text Available Im Beitrag wird die ästhetische Struktur von Tizians Vergewaltigung der Lucretia von 1571 analysiert, eines späten Gemäldes, das für Philipp II. entstand. Dieses zeigt – entgegen der Malereitradition – nicht den Selbstmord Lucretias, sondern den vorausgegangenen Gewaltakt durch Tarquinius. Das Geschehen wird dabei ambivalent dargestellt: Motivisch kehrt Tizian die Vehemenz des Übergriffs hervor, auf der ästhetischen Ebene und in der Inszenierung des Akts und seines Umfelds rekurriert er jedoch zugleich auf Darstellungen und Interpretationen, die der Frau einen Anteil an dem Verbrechen zuschreiben wollten. Diese Ambivalenz fordert im hohen Maß die Beteiligung der Betrachter/-innen an der Sinngenerierung heraus, vor allem deren Bilderkenntnis, zielt also auf einen imaginativen Bildspeicher, über den jede/r Betrachter/-in verfügt und auf den er/sie für die Interpretation realer Bilder zurückgreift.The article analyzes the aesthetic structure of Titian’s Rape of Lucretia, a late painting of the year 1517, that was done for Philip II. Contrary to the painting tradition, this work does not show Lucretia’s suicide, but Tarquinius’s preceding act of violence. The incident is presented ambivalently: motivicly, Titian emphasizes the vehemence of the attack, however, on the aesthetic level and regarding the illustration of the act and its surroundings, he also refers back to images and interpretations that want to blame part of the crime on the woman. This ambivalence highly challenges the observer’s contribution to the creation of meaning, above all their image perception, so it aims at an imaginary image memory that all observers have at their command and which they access for the interpretation of real images.

  16. Analyses of functional brain connectivity; Untersuchungen zur funktionellen Konnektivitaet des Gehirns

    Energy Technology Data Exchange (ETDEWEB)

    Stephan, K.E.

    2003-03-01

    This dissertation includes two independent studies that investigate two complementary aspects of functional connectivity in the Macaque and the human brain. In the first study, a computational meta-analysis of published electrophysiological data on context-independent functional brain connectivity was conducted by means of three independent methods. The second study investigated the effects of the atypical antipsychotic substance olanzapine on the functional connectivity of the cerebellum during a simple motor task (self-paced finger tapping). Six schizophrenic patients and six control subjects matched for age and sex were investigated by functional magnetic resonance imaging (fMRI) twice. This study provided the first experimental data on the effects of atypical antipsychotic agents on functional brain connectivity and demonstrated pronounced olanzapine-dependent changes of functional couplings between cerebellum, thalamus, and prefrontal cortex. (orig.) [German] In der hier vorgelegten Arbeit werden zwei komplementaere Aspekte der funktionellen Konnektivitaet - im Gehirn des Makaken und Menschen anhand zweier separater Studien untersucht. In der ersten Studie wurde mittels dreier unabhaengiger Methoden eine Metaanalyse publizierter elektrophysiologischer Daten zur kontextunabhaengigen funktionellen Konnektivitaet des Makakenkortex durchgefuehrt. Diese Studie erbrachte damit zum ersten Mal den Nachweis einer funktionellen Small World-Netzwerkstruktur des Primatenkortex. In der zweiten Studie wurde der Effekt des atypischen Neuroleptikums Olanzapin auf die funktionelle Konnektivitaet des Zerebellums im Kontext einer einfachen motorischen Aufgabe (selbstgesteuertes Fingertrapping) untersucht. Sechs schizophrene Patienten, die Neuroleptika-naiv bzw. -entwoehnt waren, sowie sechs alters- und geschlechtsentprechende Kontrollprobanden wurden im Abstand von jeweils drei Wochen mit funktioneller Magnetresonanztomografie (fMRT) untersucht. Diese Studie lieferte die ersten

  17. Ein Beitrag zur aquatischen Käferfauna von Walcheren, Niederlande (Insecta, Coleoptera) unter besonderer Berücksichtigung von Halophilen und Halobionten Arten

    NARCIS (Netherlands)

    Burmeister, E.-G.

    1982-01-01

    EINLEITUNG In den Jahren 1975, 1977 und 1978 konnten in den Sommermonaten zahlreiche Gewässer der Halbinsel Walcheren auf ihre aquatische Käferfauna hin untersucht werden. Walcheren, ehemals eine Insel in der Scheidemündung, gehört zur Provinz Zeeland und ist heute durch die Polderlandschaft

  18. Re-Imagining "Bildung Zur Humanität": How I Developed the Dialogos Approach to Practical Philosophy through Action Inquiry Research

    Science.gov (United States)

    Helskog, Guro Hansen

    2015-01-01

    This paper presents an account of how I developed the Dialogos approach to practical philosophy through action inquiry research. The process of development is understood as a contribution to the reconstruction of the notion "Bildung zur Humanität" as an ideal in education. Core perspectives, traditions and purposes involved in the action…

  19. Zwischen Wildnis und Freizeitpark: Eine Streitschrift zur Zukunft der Alpen [Between Wilderness and Leisure Park: A Pamphlet on the Future of the Alps

    Directory of Open Access Journals (Sweden)

    Dominik Siegrist

    2016-02-01

    Full Text Available Reviewed: Zwischen Wildnis und Freizeitpark: Eine Streitschrift zur Zukunft der Alpen [Between Wilderness and Leisure Park: A Pamphlet on the Future of the Alps] By Werner Bätzing. Zurich, Switzerland: Rotpunktverlag, 2015. 145 pp. € 9.90. ISBN 978-3-85869-648-9.

  20. Studies on the hazard potential of noble metal emissions from catalytic converters; Untersuchungen zur Frage des Gefaehrdungspotentials von katalysatorbuertigen Edelmetallemissionen (VPT 05)

    Energy Technology Data Exchange (ETDEWEB)

    Artelt, S. [Fraunhofer-Institut fuer Toxikologie und Aerosolforschung, Hannover (Germany)

    1997-12-31

    Plants were exposed to engine exhaust fumes from engine test stands in order to estimate the probable level of platinum deposition near roads. [Deutsch] Zur Abschaetzung der zu erwarteten Platindeposition in der Naehe von Strassen wurden Pflanzen mit Motorabgasen, die aus Motorstandexperimenten gewonnen wurden, beaufschlagt. (orig.)

  1. Zur Erfassung hygrothermischer Einflüsse auf das nichtlineare Trag- und Schädigungsverhalten von Stahlbetonflächentragwerken

    OpenAIRE

    Wörmann, Ralf

    2004-01-01

    Während ihrer Nutzungsdauer werden Stahlbetontragwerke neben den mechanischen Belastungen auch durch weitere umgebungs- und betriebsbedingte Einwirkungen beansprucht. Die Schadensdiagnostik an bestehenden Konstruktionen zeigt, dass Schädigungen häufig durch ein Zusammenwirken der dynamischen Einwirkungen aus Wind und Verkehr mit den hygrisch und thermisch induzierten Zwang- und Eigenbeanspruchungen verursacht werden. Zur numerischen Simulation derartiger Schädigungsprozesse ...

  2. Electromagnetic and ultrasonic techniques to evaluate stress states of components; Elektromagnetische und Ultraschallverfahren zur Spannungsanalyse an Bauteilen

    Energy Technology Data Exchange (ETDEWEB)

    Schneider, E.; Kern, R.; Theiner, W.A. [Fraunhofer Inst. fuer Zerstoerungsfreie Pruefverfahren, IZFP, Saarbruecken (Germany)

    1999-08-01

    The electromagnetic and ultrasonic techniques are comparably recent NDT methods for determination of stress states of components. They are simple in application, but require pre-measurement preparation: Electromagnetic techniques need calibration, and quantitative stress analysis by ultrasonic techniques needs reference values, i.e. verified materials-specific quantities to be obtained with representative specimens. Electromagnetic and ultrasonic techniques have been developed for specific tests at defined components, and the corresponding instruments and sensors have been used in practice for several years now. The paper summarizes fundamental aspects and explains the state of the art by means of several examples. (orig./CB) [Deutsch] Elektromagnetische und Ultraschallverfahren sind vergleichsweise neue zerstoerungsfreie Verfahren zur Bestimmung von Eigenspannungen in Bauteilen. Ihre Anwendung ist einfach, setzt aber Vorarbeiten voraus: Elektromagnetische Verfahren muessen kalibriert und zur quantitativen Spannungsanalyse mittels Ultraschallverfahren muessen materialspezifische Kenngroessen an repraesentativen Materialproben ermittelt werden. Elektromagnetische und Ultraschallverfahren sind fuer konkrete Anwendungen an Bauteilen entwickelt, angepasste Geraete und Sensoren seit Jahren in der Nutzung. Der Beitrag fasst die Grundlagen zusammen und stellt den Stand der Technik anhand ausgewaehlter Anwendungen dar. (orig.)

  3. Zur Entwicklung eines interaktiven Verfahrens der Sprachstandsermittlung bei mehrsprachig aufwachsenden Kindern – von der Idee zu ersten Umsetzungsschritten

    Directory of Open Access Journals (Sweden)

    Jörg Roche et al.

    2016-10-01

    Full Text Available Dieser Beitrag skizziert ein Konzept zur Sprachstandsermittlung, das aufbauend auf Ergebnissen der gebrauchsbasierten Spracherwerbsforschung die sprachlichen Fähigkeiten von Vorschulkindern in quasi-natürlichen Gesprächssituationen misst. Den Maßstab bilden dabei die altersgemäßen Deutschkompetenzen monolingualer und bilingualer Kinder im Vorschulalter. Mit dem neuen Verfahren soll eine akkurate Bewertung der kommunikativen Kompetenzen der Kinder am Übergang zur Schule erfolgen, die eine systematische Benachteiligung mehrsprachig aufwachsender Kinder aufgrund von Vergleichen mit zielsprachlichen Erwachsenennormen vermeidet. Das neuartige Erhebungsverfahren wird dabei in Form einer digitalen Spielumgebung (App entwickelt, die eine Umsetzung der Prinzipien des handlungsorientierten Sprachenlernens erlaubt. This paper presents the implementation of a new concept of first and second language assessment for pre-school children between four and six years. The concept is theoretically grounded in usage-based linguistics. It assesses language in authentic communicative settings in a game based app. The settings provide the children with incentives to engage in communication. As a result, the assessment tool does not evaluate formal aspects of language. Instead it relies on functional categories for evaluation. Norms for evaluations are derived from longitudinal corpus studies of authentic child language production. The goal of the new approach is to generate an accurate and fair assessments of the children’s communicative capacities. Pilot studies indicate that children with L2 German appear to be more advanced than shown by traditional form-based assessment methods.

  4. "Also dieses enge Korsett ist nicht mehr da." Zur Zeitsouveränität im Alter

    Directory of Open Access Journals (Sweden)

    Anne Münch

    2014-09-01

    Full Text Available Mit dem Übergang in den Ruhestand sind ältere Menschen mit der Aufgabe konfrontiert, ihren Alltag und die durch den Wegfall der Erwerbsarbeit zusätzlich zur Verfügung stehende Zeit neu zu arrangieren. Dies geschieht auf vielfältige Weise, manchmal mehr, manchmal weniger zufriedenstellend. Der vorliegende Beitrag präsentiert die empirischen Ergebnisse einer qualitativen Forschungsarbeit zur Zeitgestaltung im Alter und rekonstruiert die subjektiven Deutungen von Zeitsouveränität im Ruhestandsalltag. In Orientierung an der Methodologie der Grounded Theory konnten aus dem Interviewmaterial heraus insgesamt drei Strategien des Umgangs mit der Zeitsouveränität im Alter entwickelt werden, die mit unterschiedlichen Zeiterfahrungen und -kompetenzen in Zusammenhang stehen und auf die Bedeutung der Erwerbsbiografie über den Ruhestandseintritt hinaus verweisen. Unter Einfluss der jeweiligen Kontextbedingungen neigen die Individuen entweder dazu, nach dem Übergang in die Posterwerbsphase die Zeit zu genießen, die Zeit auszufüllen oder aber ihre Zeit zu investieren. URN: http://nbn-resolving.de/urn:nbn:de:0114-fqs1403193

  5. Autologous osteochondral mosaicplasty or TruFit plugs for cartilage repair.

    Science.gov (United States)

    Hindle, Paul; Hendry, Jane L; Keating, John F; Biant, Leela C

    2014-06-01

    Autologous osteochondral mosaicplasty and TruFit Bone graft substitute plugs are methods used to repair symptomatic articular cartilage defects in the adult knee. There have been no comparative studies of the two techniques. This retrospective study assessed functional outcome of patients using the EQ-5D, Knee Injury and Osteoarthritis Outcome Score (KOOS) and Modified Cincinnati scores at follow-up of 1-5 years. There were 66 patients in the study (35 TruFit and 31 Mosaicplasty): 44 males and 22 females with a mean age of 37.3 years (SD 12.6). The mean BMI was 26.8. Thirty-six articular cartilage lesions were due to trauma, twenty-six due to osteochondritis dissecans and three due to non-specific degenerative change or unknown. There was no difference between the two groups age (n.s.), sex (n.s.), BMI (n.s.), defect location (n.s.) or aetiology (n.s.). The median follow-up was 22 months for the TruFit cohort and 30 months for the mosaicplasty group. There was no significant difference in the requirement for re-operation (n.s). Patients undergoing autologous mosaicplasty had a higher rate of returning to sport (p = 0.006), lower EQ-5D pain scores (p = 0.048) and higher KOOS activities of daily living (p = 0.029) scores. Sub-group analysis showed no difference related to the number of cases the surgeon performed. Patients requiring re-operation had lower outcome scores regardless of their initial procedure. This study demonstrated significantly better outcomes using two validated outcome scores (KOOS, EQ-5D), and an ability to return to sport in those undergoing autologous mosaicplasty compared to those receiving TruFit plugs. IV.

  6. Autologous Transfusion of Stored Red Blood Cells Increases Pulmonary Artery Pressure

    Science.gov (United States)

    Pinciroli, Riccardo; Stowell, Christopher P.; Wang, Lin; Yu, Binglan; Fernandez, Bernadette O.; Feelisch, Martin; Mietto, Cristina; Hod, Eldad A.; Chipman, Daniel; Scherrer-Crosbie, Marielle; Bloch, Kenneth D.; Zapol, Warren M.

    2014-01-01

    Rationale: Transfusion of erythrocytes stored for prolonged periods is associated with increased mortality. Erythrocytes undergo hemolysis during storage and after transfusion. Plasma hemoglobin scavenges endogenous nitric oxide leading to systemic and pulmonary vasoconstriction. Objectives: We hypothesized that transfusion of autologous blood stored for 40 days would increase the pulmonary artery pressure in volunteers with endothelial dysfunction (impaired endothelial production of nitric oxide). We also tested whether breathing nitric oxide before and during transfusion could prevent the increase of pulmonary artery pressure. Methods: Fourteen obese adults with endothelial dysfunction were enrolled in a randomized crossover study of transfusing autologous, leukoreduced blood stored for either 3 or 40 days. Volunteers were transfused with 3-day blood, 40-day blood, and 40-day blood while breathing 80 ppm nitric oxide. Measurements and Main Results: The age of volunteers was 41 ± 4 years (mean ± SEM), and their body mass index was 33.4 ± 1.3 kg/m2. Plasma hemoglobin concentrations increased after transfusion with 40-day and 40-day plus nitric oxide blood but not after transfusing 3-day blood. Mean pulmonary artery pressure, estimated by transthoracic echocardiography, increased after transfusing 40-day blood (18 ± 2 to 23 ± 2 mm Hg; P transfusing 3-day blood (17 ± 2 to 18 ± 2 mm Hg; P = 0.5). Breathing nitric oxide decreased pulmonary artery pressure in volunteers transfused with 40-day blood (17 ± 2 to 12 ± 1 mm Hg; P Transfusion of autologous leukoreduced blood stored for 40 days was associated with increased plasma hemoglobin levels and increased pulmonary artery pressure. Breathing nitric oxide prevents the increase of pulmonary artery pressure produced by transfusing stored blood. Clinical trial registered with www.clinicaltrials.gov (NCT 01529502). PMID:25162920

  7. Autologous chondrocytes as a novel source for neo-chondrogenesis in haemophiliacs.

    Science.gov (United States)

    Stocco, Elena; Barbon, Silvia; Radossi, Paolo; Rajendran, Senthilkumar; Dalzoppo, Daniele; Bortolami, Marina; Bagno, Andrea; Grandi, Francesca; Gamba, Pier Giorgio; Parnigotto, Pier Paolo; Tagariello, Giuseppe; Grandi, Claudio

    2016-10-01

    Haemophilic arthropathy is the major cause of disability in patients with haemophilia and, despite prophylaxis with coagulation factor concentrates, some patients still develop articular complications. We evaluate the feasibility of a tissue engineering approach to improve current clinical strategies for cartilage regeneration in haemophiliacs by using autologous chondrocytes (haemophilic chondrocytes; HaeCs). Little is known about articular chondrocytes from haemophilic patients and no characterisation has as yet been performed. An investigation into whether blood exposure alters HaeCs should be interesting from the perspective of autologous implants. The typical morphology and expression of specific target genes and surface markers were therefore assessed by optical microscopy, reverse transcription plus the polymerase chain reaction (PCR), real-time PCR and flow-cytometry. We then considered chondrocyte behaviour on a bio-hybrid scaffold (based on polyvinyl alcohol/Wharton's jelly) as an in vitro model of articular cartilage prosthesis. Articular chondrocytes from non-haemophilic donors were used as controls. HaeC morphology and the resulting immunophenotype CD44(+)/CD49c(+)/CD49e(+)/CD151(+)/CD73(+)/CD49f(-)/CD26(-) resembled those of healthy donors. Moreover, HaeCs were active in the transcription of genes involved in the synthesis of the extracellular matrix proteins of the articular cartilage (ACAN, COL1A, COL2A, COL10A, COL9A, COMP, HAS1, SOX9), although the over-expression of COL1A1, COL10A1, COMP and HAS was observed. In parallel, the composite scaffold showed adequate mechanical and biological properties for cartilage tissue engineering, promoting chondrocyte proliferation. Our preliminary evidence contributes to the characterisation of HaeCs, highlighting the opportunity of using them for autologous cartilage implants in patients with haemophilia.

  8. Enteric Neural Cells From Hirschsprung Disease Patients Form Ganglia in Autologous Aneuronal ColonSummary

    Directory of Open Access Journals (Sweden)

    Benjamin N. Rollo

    2016-01-01

    Full Text Available Background & Aims: Hirschsprung disease (HSCR is caused by failure of cells derived from the neural crest (NC to colonize the distal bowel in early embryogenesis, resulting in absence of the enteric nervous system (ENS and failure of intestinal transit postnatally. Treatment is by distal bowel resection, but neural cell replacement may be an alternative. We tested whether aneuronal (aganglionic colon tissue from patients may be colonized by autologous ENS-derived cells. Methods: Cells were obtained and cryopreserved from 31 HSCR patients from the proximal resection margin of colon, and ENS cells were isolated using flow cytometry for the NC marker p75 (nine patients. Aneuronal colon tissue was obtained from the distal resection margin (23 patients. ENS cells were assessed for NC markers immunohistologically and by quantitative reverse-transcription polymerase chain reaction, and mitosis was detected by ethynyl-2′-deoxyuridine labeling. The ability of human HSCR postnatal ENS-derived cells to colonize the embryonic intestine was demonstrated by organ coculture with avian embryo gut, and the ability of human postnatal HSCR aneuronal colon muscle to support ENS formation was tested by organ coculture with embryonic mouse ENS cells. Finally, the ability of HSCR patient ENS cells to colonize autologous aneuronal colon muscle tissue was assessed. Results: ENS-derived p75-sorted cells from patients expressed multiple NC progenitor and differentiation markers and proliferated in culture under conditions simulating Wnt signaling. In organ culture, patient ENS cells migrated appropriately in aneural quail embryo gut, and mouse embryo ENS cells rapidly spread, differentiated, and extended axons in patient aneuronal colon muscle tissue. Postnatal ENS cells derived from HSCR patients colonized autologous aneuronal colon tissue in cocultures, proliferating and differentiating as neurons and glia. Conclusions: NC-lineage cells can be obtained from HSCR

  9. Autologous breast reconstruction using the immediately lipofilled extended latissimus dorsi flap.

    Science.gov (United States)

    Johns, N; Fairbairn, N; Trail, M; Ewing, A; Yong, L; Raine, C; Dixon, J M

    2018-02-01

    The latissimus dorsi flap is a popular choice for autologous breast reconstruction. To dramatically improve volume, we report our experience of using the immediately lipofilled extended latissimus dorsi (ELD) flap and show it as a valid option for autologous breast reconstruction. Patients undergoing the procedure between December 2013 and June 2016 were included. Demographic, clinical and operative factors were analysed, together with in-hospital morbidity and duration of postoperative hospital stay. A total of 71 ELD flaps with immediate lipofilling were performed. Forty-five reconstructions were immediate and the remaining 26 delayed. Median (range) volume of autologous fat injected immediately was 171 ml (40-630 ml). Contralateral reductions were performed in 25 patients with the median reduction volume 185 g (89-683 g). Median duration of admission was 6.5 (3-18) days and patients were followed up for 12 months (1-37). Three total flap failures occurred and had to be excised (4%). One haematoma occurred requiring drainage (1%). Signs of infection requiring intravenous antibiotics occurred in five patients (7%). In 5 patients wound dehiscence occurred, and only two of these required resuturing (3%). In total, 7 patients developed a seroma requiring repeated drainage (10%). Three reconstructions experienced mild mastectomy flap necrosis with no needing reoperation (4%). Our experience represents the largest series to date and shows that in carefully selected patients the technique is safe, can avoid the requirement for implants, and has the potential to streamline the reconstructive journey. Copyright © 2017 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.

  10. Repair of large segmental bone defects: BMP-2 gene activated muscle grafts vs. autologous bone grafting.

    Science.gov (United States)

    Betz, Oliver B; Betz, Volker M; Schröder, Christian; Penzkofer, Rainer; Göttlinger, Michael; Mayer-Wagner, Susanne; Augat, Peter; Jansson, Volkmar; Müller, Peter E

    2013-08-08

    Common cell based strategies for the treatment of osseous defects require the isolation and expansion of autologous cells. Since this makes such approaches time-consuming and expensive, we developed a novel expedited technology creating gene activated muscle grafts. We have previously shown that large segmental bone defects in rats can be regenerated by implantation of muscle tissue fragments activated by BMP-2 gene transfer. In the present study, we compared the bone healing capacities of such gene activated muscle grafts with bone isografts, mimicking autologous bone grafting, the clinical gold standard for treatment of bone defects in patients. Two of 14 male, syngeneic Fischer 344 rats used for this experiment served as donors for muscle and bone. Muscle tissue was harvested from both hind limbs and incubated with an adenoviral vector carrying the cDNA encoding BMP-2. Bone was harvested from the iliac crest and long bone epiphyses. Bone defects (5 mm) were created in the right femora of 12 rats and were filled with either BMP-2 activated muscle tissue or bone grafts. After eight weeks, femora were evaluated by radiographs, micro-computed tomography (μCT), and biomechanical testing. In the group receiving BMP-2 activated muscle grafts as well as in the bone-grafting group, 100% of the bone defects were healed, as documented by radiographs and μCT-imaging. Bone volume was similar in both groups and biomechanical stability of the two groups was statistically indistinguishable. This study demonstrates that treatment of large bone defects by implantation of BMP-2 gene activated muscle tissue leads to similar bone volume and stability as bone isografts, mimicking autologous bone grafting.

  11. Human autologous serum as a substitute for fetal bovine serum in human Schwann cell culture.

    Directory of Open Access Journals (Sweden)

    Parisa Goodarzi

    2014-04-01

    Full Text Available Nowadays, cell -based and tissue engineered products have opened new horizons in treatment of incurable nervous system disorders. The number of studies on the role of Schwann cells (SC in treating nervous disorders is higher than other cell types. Different protocols have been suggested for isolation and expansion of SC which most of them have used multiple growth factors, mitogens and fetal bovine sera (FBS in culture medium. Because of potential hazards of animal-derived reagents, this study was designed to evaluate the effect of replacing FBS with human autologous serum (HAS on SC's yield and culture parameters. Samples from 10 peripheral nerve biopsies were retrieved and processed under aseptic condition. The isolated cells cultured in FBS (1st group or autologous serum (2nd group. After primary culture the cells were seeded at 10000 cell/cm2 in a 12 wells cell culture plate for each group. At 100% confluency, the cell culture parameters (count, viability, purity and culture duration of 2 groups were compared using paired t-test. The average donors' age was 35.80 (SD=13.35 and except for 1 sample the others cultured successfully. In first group, the averages of cell purity, viability and culture duration were 97% (SD=1.32, 97/33% (SD=1.22 and 11.77 (SD=2.58 days respectively. This parameters were 97.33% (SD=1.00, 97.55% (SD=1.33 and 10.33 days (SD=1.65 in second group. The difference of cell count, purity and viability were not significant between 2 groups (P>0.05. The cells of second group reached to 100% confluency in shorter period of time (P=0.03. The results of this study showed that autologous serum can be a good substitute for FBS in human SC culture. This can reduce the costs and improve the safety of cell product for clinical application.

  12. Revision surgery after third generation autologous chondrocyte implantation in the knee.

    Science.gov (United States)

    Niethammer, Thomas R; Niethammer, Thomas; Valentin, Siegfried; Ficklscherer, Andreas; Gülecyüz, Mehmet F; Gülecyüz, Mehmet; Pietschmann, Matthias F; Pietschmann, Matthias; Müller, Peter E; Müller, Peter

    2015-08-01

    Third generation autologous chondrocyte implantation (ACI) is an established treatment for full thickness cartilage defects in the knee joint. However, little is known about cases when revision surgery is needed. The aim of the present study is to investigate the complication rates and the main reasons for revision surgery after third generation autologous chondrocyte implantation in the knee joint. It is of particular interest to examine in which cases revision surgery is needed and in which cases a "wait and see" strategy should be used. A total of 143 consecutive patients with 171 cartilage defects were included in this study with a minimum follow-up of two years. All defects were treated with third generation ACI (NOVACART®3D). Clinical evaluation was carried out after six months, followed by an annual evaluation using the International Knee Documentation Committee (IKDC) subjective score and the visual analogue scale (VAS) for rest and during activity. Revision surgery was documented. The revision rate was 23.4 % (n = 36). The following major reasons for revision surgery were found in our study: symptomatic bone marrow edema (8.3 %, n = 3), arthrofibrosis (22.2 %, n = 8) and partial graft cartilage deficiency (47.2 %, n = 17). The following revision surgery was performed: retrograde drilling combined with Iloprost infusion therapy for bone marrow oedema (8.4 %, n = 3), arthroscopic arthrolysis of the suprapatellar recess (22.2 %, n = 8) and microfracturing/antegrade drilling (47.3 %, n = 17). Significant improvements of clinical scores after revision surgery were observed. Revision surgery after third generation autologous chondrocyte implantation is common and is needed primarily in cases with arthrofibrosis, partial graft cartilage deficiency and symptomatic bone marrow oedema resulting in a significantly better clinical outcome.

  13. Treatment of lower extremity long bone nonunion with expandable intramedullary nailing and autologous bone grafting.

    Science.gov (United States)

    Niu, Yunfei; Bai, Yushu; Xu, Shuogui; Liu, Xinwei; Wang, Panfeng; Wu, Dajiang; Zhang, Chuncai; Li, Ming

    2011-07-01

    Nonunion of long bones in lower limbs is a common complication of orthopedic trauma that can be extremely debilitating. This retrospective study describes our experience using expandable intramedullary nails and autologous bone grafting in treating lower limb long bone nonunion with bone defects. Nineteen patients (mean age 38.9 years, range 18-61) with lower limb long bone nonunion and defects caused by femoral or tibial fracture types were as follows: A2 (3 femoral, 1 tibial), A3 (1 femoral, 2 tibial), B2 (3 femoral, 4 tibial), and B3 (1 femoral, 4 tibial). Expandable intramedullary nailing and autologous bone (iliac and/or fibular) grafting were used for the treatment. Postoperative bone healing as determined by analysis of standard anteroposterior and lateral X-ray films every 4 weeks. Complications were noted. The average number of previous surgeries was 1.9 (range 1-4). The mean duration from original injury to treatment was 17.6 months (range 9-40 months). Femoral shaft nonunion healed on average of 26.5 weeks (range 16-60 weeks) after surgery, while tibial shaft nonunion healed on average of 23.6 weeks (range 12-40 weeks) after surgery. Class I healing occurred in all but two patients who experienced chronic postoperative osteomyelitis and delayed wound healing, respectively. Two patients complained of postoperative donor site pain. The use of expandable intramedullary nails and autologous bone grafts was an effective method for repair of nonunion of lower limb fractures combining with bone defects with minimal complications.

  14. Cellulite: a new treatment approach combining subdermal Nd: YAG laser lipolysis and autologous fat transplantation.

    Science.gov (United States)

    Goldman, Alberto; Gotkin, Robert H; Sarnoff, Deborah S; Prati, Clarissa; Rossato, Flávia

    2008-01-01

    Cellulite is an alteration of the topography of the skin that occurs in body areas where fat deposition seems to be under the influence of estrogen: mainly the hips, buttocks, thighs, and abdomen. The presence of cellulite is a significant source of patient dissatisfaction. There is currently no cure or consistently effective treatment for cellulite. The authors sought to show that the subdermal application of the neodymium-doped yttrium aluminium garnet (Nd:YAG) laser combined with autologous fat transplantation is a safe and effective treatment for cellulite. From January 2003 to December 2006, 52 female patients with Curri grade III to IV cellulite were treated with subdermal 1064-nm Nd:YAG laser lipolysis combined with autologous fat transplantation. Patient assessment was collected for data analysis. After the treatment, tissue samples were obtained in some subjects in order to ascertain the histologic effects of the laser treatment. This treatment resulted in significant clinical improvement in cellulite. The adverse effects were mild and temporary, and the postoperative period was well tolerated. A majority of patients (84.6%) rated the results of treatment as either good or excellent. The treatment of severe cases of cellulite (Curri grades III and IV) by a combination of 1064-nm Nd:YAG laser lipolysis and autologous fat transplantation proved to be both safe and effective. In addition, subdermal laser lipolysis has the advantage of inducing neocollagenesis and stimulating postoperative skin tightening. This represents a new treatment option for the ubiquitous cellulite disorder. Although this treatment has shown promising results in this pilot study, further studies are necessary in order to draw final conclusions.

  15. The autologous pleural buttressing of staple lines in surgery for bullous lung disease.

    Science.gov (United States)

    Baysungur, Volkan; Tezel, Cagatay; Ergene, Gokhan; Sevilgen, Gokcen; Okur, Erdal; Uskul, Bahadir; Halezeroglu, Semih

    2010-12-01

    Prolonged air leak remains as one of the most common complications after surgery for bullous lung disease. Reinforcement of the staple line with either prosthetic material or bovine pericardial strips has been advocated to avoid this problem. We used the patients' own parietal pleural layer to cover the staple lines to prevent air leak and subsequently assessed the comparative results. A total of 22 patients underwent thoracotomy for bullous lung disease, mainly due to lobe-dominance bullae combined with emphysema, between November 2006 and November 2008. A case-control study was set from the surgical data of patients who were operated on using stapling devices without any buttressing material (group I=12) and were compared with the group of patients who were operated on using staplers buttressed with an autologous pleural layer (group II=10). Patient characteristics, chest-tube removal time and length of hospital stay were prospectively recorded in group II. The outcomes of the two groups were analysed based on postoperative complications, chest-tube removal time and postoperative length of hospital stay. There was no statistically significant difference between both the groups in preoperative characteristics including age, sex, co-morbid factors, and respiratory functions, heterogeneity of emphysema, intra-operative adhesion density and length of staple line. However, the chest tube was removed significantly earlier in patients whose bullae were resected by stapling devices buttressed with autologous-parietal pleura (p=0.04). Autologous pleural reinforcement of the staple line in surgery for bullous lung disease is a safe, effective and cost-free procedure that precipitates the early removal of the chest tube. Copyright © 2010 European Association for Cardio-Thoracic Surgery. Published by Elsevier B.V. All rights reserved.

  16. Evaluation of dermal substitute in a novel co-transplantation model with autologous epidermal sheet.

    Directory of Open Access Journals (Sweden)

    Guofeng Huang

    Full Text Available The development of more and more new dermal substitutes requires a reliable and effective animal model to evaluate their safety and efficacy. In this study we constructed a novel animal model using co-transplantation of autologous epidermal sheets with dermal substitutes to repair full-thickness skin defects. Autologous epidermal sheets were obtained by digesting the basement membrane (BM and dermal components from rat split-thickness skins in Dispase II solution (1.2 u/ml at 4 °C for 8, 10 and 12 h. H&E, immunohistochemical and live/dead staining showed that the epidermal sheet preserved an intact epidermis without any BM or dermal components, and a high percentage of viable cells (92.10 ± 4.19% and P63 positive cells (67.43 ± 4.21% under an optimized condition. Porcine acellular dermal matrixes were co-transplanted with the autologous epidermal sheets to repair full-thickness skin defects in Sprague-Dawley rats. The epidermal sheets survived and completely re-covered the wounds within 3 weeks. Histological staining showed that the newly formed stratified epidermis attached directly onto the dermal matrix. Inflammatory cell infiltration and vascularization of the dermal matrix were not significantly different from those in the subcutaneous implantation model. Collagen IV and laminin distributed continuously at the epidermis and dermal matrix junction 4 weeks after transplantation. Transmission electron microscopy further confirmed the presence of continuous lamina densa and hemidesmosome structures. This novel animal model can be used not only to observe the biocompatibility of dermal substitutes, but also to evaluate their effects on new epidermis and BM formation. Therefore, it is a simple and reliable model for evaluating the safety and efficacy of dermal substitutes.

  17. Comparison of mechanical compressive properties of commercial and autologous fibrin glues for tissue engineering applications.

    Science.gov (United States)

    Cravens, Matthew G; Behn, Anthony W; Dragoo, Jason L

    2017-11-01

    Fibrin glues are widely used in orthopedic surgery as adhesives and hemostatic agents. We evaluated the compressive properties of selected fibrin glues in order to identify which are appropriate for tissue regeneration applications subject to compression. Uniaxial unconfined compression tests were performed on fibrin gels prepared from commercial and autologous products: (1) Evicel (Ethicon), (2) Tisseel (Baxter), (3) Angel (Arthrex), and (4) ProPlaz (Biorich). Cyclic loads were applied from 0 to 30% strain for 100cycles at 0.5Hz. Following cyclic testing, specimens were subjected to ramp displacement of 1% strain per second to 80% strain. Throughout cyclic loading, Evicel and Tisseel deformed (shortened) less than Angel at all but one time point, and deformed less than ProPlaz at cycles 10 and 20. The dynamic moduli, peak stress, and strain energy were significantly greater in Tisseel than all other groups. Evicel displayed significantly greater dynamic moduli, peak stress, and strain energy than Angel and ProPlaz. Following cyclic testing, Tisseel and Evicel were significantly less deformed than Angel. No specimens exhibited gross failure during ramp loading to 80% strain. Ramp loading trends mirrored those of cyclic loading. The tested commercial glues were significantly more resistant to compression than the autologous products. The compressive properties of Tisseel were approximately twice those of Evicel. All preparations displayed moduli multiple orders of magnitude less than that of native articular cartilage. We conclude that in knee surgeries requiring fibrin glue to undergo compression of daily activity, commercial products are preferable to autologous preparations from platelet-poor plasma, though both will deform significantly. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. MRI methods for pulmonary ventilation and perfusion imaging; Methoden der MRT zur Ventilations- und Perfusionsbildgebung der Lunge

    Energy Technology Data Exchange (ETDEWEB)

    Sommer, G. [Universitaetsspital Basel, Klinik fuer Radiologie und Nuklearmedizin, Basel (Switzerland); Bauman, G. [Universitaetsspital Basel, Klinik fuer Radiologie und Nuklearmedizin - Radiologische Physik, Basel (Switzerland)

    2016-02-15

    Detektion frueher pathologischer Veraenderungen. Standardverfahren der bildgebenden Lungendiagnostik sind die Computertomographie (CT) zur morphologischen Darstellung und die Perfusions-/Ventilationsszintigraphie bzw. ''single photon emission computed tomography'' (SPECT) zur funktionellen Diagnostik. Zur Darstellung der Lungenventilation stehen die MRT mit hyperpolarisierten Gasen, die O{sub 2}-verstaerkte MRT, die MRT mit fluorierten Gasen und die Fourier-Dekompositions-MRT (FD-MRT) zur Verfuegung. Zur Perfusionsbestimmung koennen die dynamische kontrastmittelverstaerkte MRT (DCE-MRT), das ''arterial spin labeling'' (ASL) und die FD-MRT verwendet werden. Bildgebende Verfahren erlauben einen genaueren Einblick in die Pathophysiologie der Lungenfunktion auf regionaler Ebene. Vorteile der MRT sind die fehlende Strahlenbelastung, welche die schonende Akquisition dynamischer Daten ermoeglicht sowie die Vielfalt der verfuegbaren Kontraste und damit zugaenglichen Parameter der Lungenfunktion. Ausreichende klinische Daten existieren nur fuer bestimmte Anwendungen der DCE-MRT. Fuer die uebrigen Verfahren gibt es lediglich Machbarkeitsstudien und Fallserien mit unterschiedlichem Umfang. Hyperpolarisierte Gase sind technisch bedingt nur eingeschraenkt in der Klinik anwendbar. Ein klinischer Einsatz der genannten Verfahren sollte mit Ausnahme der DCE-MRT nur innerhalb von Studien erfolgen. (orig.)

  19. Chemokine receptor polymorphism and autologous neutralizing antibody response in long-term HIV-1 infection

    DEFF Research Database (Denmark)

    Schønning, Kristian; Joost, Mette; Gram, G J

    1998-01-01

    heterozygous for the CCR5 deletion were infected with virus of NSI phenotype. In contrast, all RPI individuals who were heterozygous for the CCR5 deletion were infected with virus of SI phenotype (p = .028). Thus, a beneficial effect of having a partly nonfunctional CCR5 coreceptor may depend on the viral SI......We have previously reported that slowly progressing HIV infection (SPI) was associated with the presence of contemporaneous autologous neutralizing antibodies. In contrast, a group of individuals with more rapidly progressing infection (RPI) generally lacked these antibodies. To understand...

  20. Treatment of chronic non-healing ulcers using autologous platelet rich plasma: a case series.

    Science.gov (United States)

    Suthar, Manish; Gupta, Saniya; Bukhari, Suhail; Ponemone, Venkatesh

    2017-02-27

    Non-healing ulcers are a major health problem worldwide and have great impact at personal, professional and social levels, with high cost in terms of human and material resources. Recalcitrant non-healing ulcers are inevitable and detrimental to the lower limb and are a major cause of non-traumatic lower limb amputations. Application of autologous Platelet Rich Plasma (PRP) has been a major breakthrough for the treatment of non-healing and diabetic foot ulcers, as it is an easy and cost-effective method, and provides the necessary growth factors that enhance tissue healing. PRP is a conglomeration of thrombocytes, cytokines and various growth factors which are secreted by α-granules of platelets that augment the rate of natural healing process with decrease in time. The purpose of this case series was to evaluate the safety and efficacy of autologous platelet rich plasma for the treatment of chronic non-healing ulcers on the lower extremity. Autologous PRP was prepared from whole blood utilizing a rapid, intraoperative point-of-care system that works on the principle of density gradient centrifugation. Twenty Four (24) patients with non-healing ulcers of different etiologies, who met the inclusion criteria, were treated with single dose of subcutaneous PRP injections along with topical application of PRP gel under compassionate use. The mean age of the treated patients was 62.5 ± 13.53 years and they were followed-up for a period of 24 weeks. All the patients showed signs of wound healing with reduction in wound size, and the mean time duration to ulcer healing was 8.2 weeks. Also, an average five fold increase in the platelet concentrate was observed in the final PRP product obtained using the rapid point-of-care device, and the average platelet dose administered to the patients was 70.10 × 10 8 . This case series has demonstrated the potential safety and efficacy of autologous platelet rich plasma for the treatment of chronic non-healing ulcers. NCT

  1. Characteristic complications after autologous chondrocyte implantation for cartilage defects of the knee joint

    DEFF Research Database (Denmark)

    Niemeyer, Philipp; Pestka, Jan M; Kreuz, Peter C

    2008-01-01

    BACKGROUND: Although autologous chondrocyte implantation (ACI) is a well-established therapy for the treatment of isolated cartilage defects of the knee joint, little is known about typical complications and their treatment after ACI. HYPOTHESIS: Unsatisfactory outcome after ACI is associated...... with technique-related typical complications. STUDY DESIGN: Case series; Level of evidence, 4. METHODS: A total of 309 consecutive patients with 349 ACI procedures of the knee joint were analyzed. Three different ACI techniques were used: periosteum-covered ACI in 52 cases (14.9%), Chondrogide (Geistlich...

  2. Arch reconstruction with autologous pulmonary artery patch in interrupted aortic arch.

    Science.gov (United States)

    Lee, Won-Young; Park, Jeong-Jun

    2014-04-01

    Various surgical techniques have been developed for the repair of an interrupted aortic arch. However, tension and Gothic arch formation at the anastomotic site have remained major problems for these techniques: Excessive tension causes arch stenosis and left main bronchus compression, and Gothic arch configuration is related to cardiovascular complications. To resolve these problems, we adopted a modified surgical technique of distal aortic arch augmentation using an autologous main pulmonary artery patch. The descending aorta was then anastomosed to the augmented aortic arch in an end-to-side manner. Here, we report two cases of interrupted aortic arch that were repaired using this technique.

  3. Our Experience with Autologous Bone Marrow Stem Cell Application in Dilated Cardiomyopathy

    Directory of Open Access Journals (Sweden)

    Mukund K

    2009-01-01

    Full Text Available Background - Use of autologous bone marrow stem cell is a newly evolving treatment modality for end stage cardiac failure as reported in the literature. We report our experience with two patients with dilated cardiomyopathy who underwent this treatment after failure of maximal conventional therapy. Methods - A 29 year old Male patient with history of orthopnea and PND, with a diagnosis of dilated cardiomyopathy and echocardiographic evidence of severe LV dysfunction was referred for further treatment. His echo on admission showed EF of 17% and no other abnormal findings except elevated bilirubin levels. He was in NYHA functional class IV. He received intracoronary injection of autologous bone marrow stem cells in January 2009. 254X106 cells were injected with a CD34+ of 0.20%. His clinical condition stabilized and he was discharged home. He received a second injection of 22X106 in vitro expanded stem cells with a CD34+ of 0.72% in Aug 2009. He is now in NYHA class II-III with EF 24%. A 31year old Male patient with history of increasing shortness of breath, severe over the past 3-4 days was admitted for evaluation and treatment. His echo on admission showed EF of 20% and was in NYHA functional class IV. Coronary angiogram was normal and he was stabilized on maximal anti failure measures. He received intracoronary autologous bone marrow stem cell injection of 56X106 with a CD34+ of 0.53% in August 2009. His clinical condition stabilized over the next 10 days and he was discharged home. Conclusions - In our experience of two cases of dilated cardiomyopathy, safety of intracoronary injection of autologous bone marrow stem cells both isolated and in vitro expanded has been proven in both the cases with efficacy proven in one of the cases. Long term follow-up of these two cases and inclusion of more number of similar cases where all available conventional therapies have not resulted in significant improvement for such studies are planned.

  4. A question of ethics: selling autologous stem cell therapies flaunts professional standards.

    Science.gov (United States)

    Munsie, Megan; Hyun, Insoo

    2014-11-01

    The idea that the body's own stem cells could act as a repair kit for many conditions, including cardiac repair, underpins regenerative medicine. While progress is being made, with hundreds of clinical trials underway to evaluate possible autologous cell-based therapies, some patients and physicians are not prepared to wait and are pursuing treatments without evidence that the proposed treatments are effective, or even safe. This article explores the inherent tension between patients, practitioners and the need to regulate the development and commercialization of new cellular therapies--even when the cells come from the patient. Copyright © 2014. Published by Elsevier B.V.

  5. A question of ethics: Selling autologous stem cell therapies flaunts professional standards

    Directory of Open Access Journals (Sweden)

    Megan Munsie

    2014-11-01

    Full Text Available The idea that the body's own stem cells could act as a repair kit for many conditions, including cardiac repair, underpins regenerative medicine. While progress is being made, with hundreds of clinical trials underway to evaluate possible autologous cell-based therapies, some patients and physicians are not prepared to wait and are pursuing treatments without evidence that the proposed treatments are effective, or even safe. This article explores the inherent tension between patients, practitioners and the need to regulate the development and commercialization of new cellular therapies — even when the cells come from the patient.

  6. Optimization of fibrinogen isolation for manufacturing autologous fibrin glue for use as scaffold in tissue engineering.

    Science.gov (United States)

    Froelich, K; Pueschel, R C; Birner, M; Kindermann, J; Hackenberg, S; Kleinsasser, N H; Hagen, R; Staudenmaier, R

    2010-05-01

    Numerous manufacturing techniques for autogenous fibrin glue used as scaffold material have been described. As there is no consensus regarding the influence of chemical additives on cell biology, it was the aim of this study to establish a method for manufacturing autologous fibrin glue without any additives. The serum part was separated from whole blood. After fibrinogen precipitation, centrifugation was performed to obtain the fibrinogen pellet. Various experimental series were run to examine influences of various temperatures or substituting centrifugation for sedimentation. The method as described here is effective, simple, and performed without any additives, which could potentially influence cell biology.

  7. Tumour-infiltrating lymphocytes mediate lysis of autologous squamous cell carcinomas of the head and neck

    DEFF Research Database (Denmark)

    Hald, Jeppe; Rasmussen, N; Claesson, Mogens Helweg

    1995-01-01

    Tumour-infiltrating lymphocytes (TIL) and tumours from six patients with squamous cell carcinomas of the head and neck (SCCHN) were investigated. The six tumours all expressed major histocompatibility complex (MHC) class I antigens both in vivo and as tumor cell lines grown in vitro. In addition,...... in a MHC-class-I-restricted fashion. Thus, the results of the present study document that carcinomas of the head and neck in some patients are infiltrated by cytotoxic T cell precursors potentially capable of rejecting the autologous tumour....

  8. In-vitro chondrogenic potential of synovial stem cells and chondrocytes allocated for autologous chondrocyte implantation

    DEFF Research Database (Denmark)

    Kubosch, Eva Johanna; Heidt, Emanuel; Niemeyer, Philipp

    2017-01-01

    Purpose: The use of passaged chondrocytes is the current standard for autologous chondrocyte implantation (ACI). De-differentiation due to amplification and donor site morbidity are known drawbacks highlighting the need for alternative cell sources. Methods: Via clinically validated flow cytometry...... analysis, we compared the expression of human stem cell and cartilage markers (collagen type 2 (Col2), aggrecan (ACAN), CD44) of chondrocytes (CHDR), passaged chondrocytes for ACI (CellGenix™), bone marrow derived mesenchymal stem cells (BMSC), and synovial derived stem cells (SDSC). Results: Primary...

  9. Autologous bone graft versus demineralized bone matrix in internal fixation of ununited long bones.

    Science.gov (United States)

    Pieske, Oliver; Wittmann, Alexandra; Zaspel, Johannes; Löffler, Thomas; Rubenbauer, Bianka; Trentzsch, Heiko; Piltz, Stefan

    2009-12-15

    Non-unions are severe complications in orthopaedic trauma care and occur in 10% of all fractures. The golden standard for the treatment of ununited fractures includes open reduction and internal fixation (ORIF) as well as augmentation with autologous-bone-grafting. However, there is morbidity associated with the bone-graft donor site and some patients offer limited quantity or quality of autologous-bone graft material. Since allogene bone-grafts are introduced on the market, this comparative study aims to evaluate healing characteristics of ununited bones treated with ORIF combined with either iliac-crest-autologous-bone-grafting (ICABG) or demineralized-bone-matrix (DBM). From 2000 to 2006 out of sixty-two consecutive patients with non-unions presenting at our Level I Trauma Center, twenty patients had ununited diaphyseal fractures of long bones and were treated by ORIF combined either by ICABG- (n = 10) or DBM-augmentation (n = 10). At the time of index-operation, patients of the DBM-group had a higher level of comorbidity (ASA-value: p = 0.014). Mean duration of follow-up was 56.6 months (ICABG-group) and 41.2 months (DBM-group). All patients were clinically and radiographically assessed and adverse effects related to bone grafting were documented. The results showed that two non-unions augmented with ICABG failed osseous healing (20%) whereas all non-unions grafted by DBM showed successful consolidation during the first year after the index operation (p = 0.146). No early complications were documented in both groups but two patients of the ICABG-group suffered long-term problems at the donor site (20%) (p = 0.146). Pain intensity were comparable in both groups (p = 0.326). However, patients treated with DBM were more satisfied with the surgical procedure (p = 0.031). With the use of DBM, the costs for augmentation of the non-union-site are more expensive compared to ICABG (calculated difference: 160 euro/case). Nevertheless, this study demonstrated that the

  10. The volume-expanding effects of autologous liquid stored plasma following hemorrhage

    DEFF Research Database (Denmark)

    Bentzer, Peter; Thomas, Owain D; Westborg, Johan

    2012-01-01

    Abstract Background. Plasma use has increased since studies have suggested that early treatment with blood components in trauma with severe hemorrhage may improve outcome. Plasma is also commonly used to correct coagulation disturbances in non-bleeding patients. Little is known about the effects...... of plasma transfusion on plasma volume. We report a prospective interventional study in which the plasma volume-expanding effect of autologous plasma was investigated after a controlled hemorrhage. Methods. Plasma obtained by plasmapheresis from nine healthy regular blood donors was stored at 2-6°C. Five...

  11. Use of 111In-labeled autologous leukocytes to image an abdominal abscess in a horse

    International Nuclear Information System (INIS)

    Koblik, P.D.; Lofstedt, J.; Jakowski, R.M.; Johnson, K.L.

    1985-01-01

    Indium 111-labeled autologous leukocytes were used to image an abdominal abscess in a horse with a palpable abdominal mass and history of Streptococcus equi infection. A focal area of radioactivity was identified in the location corresponding to the abscess. Imaging of this focal uptake was optimal 48 hours after injection. Similar scans obtained in 2 clinically normal horses revealed no evidence of focal radioactivity in this region. The cell labeling procedure gave acceptable labeling efficiency (87.5%) but an excessive number of damaged WBC, resulting in persistent lung radioactivity on all images. No adverse effects were noted. Radiation measured in the horse and its excreta were well within acceptable limits

  12. Use of In-labeled autologous leukocytes to image an abdominal abscess in a horse

    Energy Technology Data Exchange (ETDEWEB)

    Koblik, P.D.; Lofstedt, J.; Jakowski, R.M.; Johnson, K.L.

    1985-06-15

    Indium 111-labeled autologous leukocytes were used to image an abdominal abscess in a horse with a palpable abdominal mass and history of Streptococcus equi infection. A focal area of radioactivity was identified in the location corresponding to the abscess. Imaging of this focal uptake was optimal 48 hours after injection. Similar scans obtained in 2 clinically normal horses revealed no evidence of focal radioactivity in this region. The cell labeling procedure gave acceptable labeling efficiency (87.5%) but an excessive number of damaged WBC, resulting in persistent lung radioactivity on all images. No adverse effects were noted. Radiation measured in the horse and its excreta were well within acceptable limits.

  13. The effect of autologous platelet rich plasma in treatment lateral epicondylitis

    OpenAIRE

    İsmail Ağır; Barış Çaypınar; Osman Mert Topkar; Mustafa Karahan

    2011-01-01

    Lateral epicondylitis (tennis elbow) is the most commonly diagnosed cause of lateral elbow pain. The aim of this study was to compare the effect of single dose corticosteroid and autologous platelet rich plasma (PRP) injection in the treatment of tennis elbow.Materials and methods: The 15 elbow of 15 patients (6 male and 9 female) was included in the study, who applied to our clinic with lateral elbow pain and diagnosed as lateral epicondylitis. A single dose of 0,5 ml Bethametasone and 0,5 m...

  14. Neck Rhabdoid Tumors: Clinical Features and Consideration of Autologous Stem Cell Transplant.

    Science.gov (United States)

    Wolfe, Adam D; Capitini, Christian M; Salamat, Shahriar M; DeSantes, Kenneth; Bradley, Kristin A; Kennedy, Tabassum; Dehner, Louis P; Patel, Neha J

    2018-01-01

    Extrarenal malignant rhabdoid tumors (MRT) have a poor prognosis despite aggressive therapy. Adding high-dose chemotherapy with autologous stem cell rescue (HDC-ASCR) as consolidative therapy for MRT is controversial. We describe 2 patients, age 13 years and 19 months, with unresectable neck MRT. After chemotherapy and radiotherapy, both underwent HDC-ASCR and remain in remission over 4 years later. We reviewed all published cases of neck MRT, and found poorer outcomes and more variable age of presentation and time to progression than MRT at other sites. Neck MRT may represent a higher-risk subset of MRT, and addition of HDC-ASCR merits consideration.

  15. Human CD56+ cytotoxic lung lymphocytes kill autologous lung cells in chronic obstructive pulmonary disease.

    Directory of Open Access Journals (Sweden)

    Christine M Freeman

    Full Text Available CD56+ natural killer (NK and CD56+ T cells, from sputum or bronchoalveolar lavage of subjects with chronic obstructive pulmonary disease (COPD are more cytotoxic to highly susceptible NK targets than those from control subjects. Whether the same is true in lung parenchyma, and if NK activity actually contributes to emphysema progression are unknown. To address these questions, we performed two types of experiments on lung tissue from clinically-indicated resections (n = 60. First, we used flow cytometry on fresh single-cell suspension to measure expression of cell-surface molecules (CD56, CD16, CD8, NKG2D and NKp44 on lung lymphocytes and of the 6D4 epitope common to MICA and MICB on lung epithelial (CD326+ cells. Second, we sequentially isolated CD56+, CD8+ and CD4+ lung lymphocytes, co-cultured each with autologous lung target cells, then determined apoptosis of individual target cells using Annexin-V and 7-AAD staining. Lung NK cells (CD56+ CD3- and CD56+ T cells (CD56+ CD3+ were present in a range of frequencies that did not differ significantly between smokers without COPD and subjects with COPD. Lung NK cells had a predominantly "cytotoxic" CD56+ CD16+ phenotype; their co-expression of CD8 was common, but the percentage expressing CD8 fell as FEV1 % predicted decreased. Greater expression by autologous lung epithelial cells of the NKG2D ligands, MICA/MICB, but not expression by lung CD56+ cells of the activating receptor NKG2D, correlated inversely with FEV1 % predicted. Lung CD56+ lymphocytes, but not CD4+ or CD8+ conventional lung T cells, rapidly killed autologous lung cells without additional stimulation. Such natural cytotoxicity was increased in subjects with severe COPD and was unexplained in multiple regression analysis by age or cancer as indication for surgery. These data show that as spirometry worsens in COPD, CD56+ lung lymphocytes exhibit spontaneous cytotoxicity of autologous structural lung cells, supporting their

  16. Repair of an Autologous Saphenous Vein Graft Aneurysm Ten Years after Renal Artery Reconstruction during Live Donor Renal Transplantation.

    Science.gov (United States)

    Oelschlaeger, Markus; Sokolakis, Ioannis; Kalogirou, Charis; Frey, Lea; Riedmiller, Hubertus; Kübler, Hubert; Kellersmann, Richard; Vergho, Daniel

    2017-10-06

    Saphenous vein graft (SVG) aneurysms (SVGA) after renal transplantation represents a rare vascular complication with subsequent challenging multidisciplinary treatment. We present a case of a 30-year-old female who received a live donor kidney transplantation for end-stage renal disease that was caused due to the hemolytic uremic syndrome. Postoperatively, an insufficient graft perfusion due to an arterial kinking was noted and repaired using an autologous SVG interposition. Ten years later, a 3-cm aneurysm of the SVG at the anastomotic site with the common iliac artery was discovered. Multidisciplinary surgical exploration with excision of the aneurysm-carrying vein graft and interposition of a new autologous SVG was successfully carried out with preservation of renal allograft's function. Treatment of SVGA after rental transplantation with a new autologous SVG is challenging but feasible, requiring a multidisciplinary approach in order to guarantee successful rates and to prevent allograft loss. © 2017 S. Karger AG, Basel.

  17. Autologous HIV-1 neutralizing antibodies: emergence of neutralization-resistant escape virus and subsequent development of escape virus neutralizing antibodies

    DEFF Research Database (Denmark)

    Arendrup, M; Nielsen, C; Hansen, J E

    1992-01-01

    The capacity of consecutive human sera to neutralize sequentially obtained autologous virus isolates was studied. HIV-1 was isolated three times over a 48-164-week period from three individuals immediately after seroconversion and from two individuals in later stages of infection. Development of ...... escape virus may be part of the explanation of the apparent failure of the immune system to control HIV infection.......The capacity of consecutive human sera to neutralize sequentially obtained autologous virus isolates was studied. HIV-1 was isolated three times over a 48-164-week period from three individuals immediately after seroconversion and from two individuals in later stages of infection. Development...... of neutralizing antibodies to the primary virus isolates was detected 13-45 weeks after seroconversion. Emergence of escape virus with reduced sensitivity to neutralization by autologous sera was demonstrated. The patients subsequently developed neutralizing antibodies against the escape virus but after a delay...

  18. High dose ACNU and radiation therapy with autologous bone marrow rescue for a patient with cerebellar medulloblastoma

    International Nuclear Information System (INIS)

    Matsumoto, Masahito; Yamashita, Junkoh; Okamoto, Shin-ichiro; Handa, Hajime; Sawada, Hitoshi; Abe, Mitsuyuki

    1984-01-01

    High dose ACNU and radiation therapy with auto-logous bone marrow rescue was performed in a 3-year-old boy suffering from cerebellar medulloblastoma, whose main mass had been removed at operation when widespread subarachnoid tumor dissemination was already present. The myelosuppression, which is a major side effect of high dose chemotherapy, was successfully prevented by the autologous bone marrow grafting and the serial CT scans showed complete dissappearance of the tumor. However, the patient died on the 53rd day after the administration of ACNU of respiratory complication which was most likely due to pulmonary fibrosis. Although the autologous bone marrow rescue therapy is a technical advance to cope with myelosuppression secondary to chemotherapy, side effects of the other organs, particularly of the respiratory system, remain to be solved. The optimal treatment schedule should be established as soon as possible. (author)

  19. Infected bone inactivation combined with transplantation of autologous platelet-rich plasma and bone marrow for treatment of chronic osteomyelitis.

    Science.gov (United States)

    Wang, J-H; Zhao, K; Liu, H-L; Zhao, H-M; Yang, J; Sun, X-K

    2015-12-01

    Here we tested the therapeutic efficacy of infected bone inactivation combined with transplantation of autologous platelet-rich plasma and bone marrow in chronic osteomyelitis. 64 patients with chronic osteomyelitis were randomly divided into two groups. Patients in control group received conventional antibiotic and surgical treatments, while patients in the experimental treatment group underwent infected bone inactivation combined with transplantation of autologous platelet-rich plasma and bone marrow. The X-ray, histological, and biochemical (alkaline phosphatase) changes were assessed at 4, 8, 12 and 16 weeks after the treatment. At all tested study points, X-ray and histological scores, and alkaline phosphatase levels were significantly better in patients of the experimental treatment group. Infected bone inactivation combined with transplantation of autologous platelet-rich plasma and bone marrow achieves beneficial therapeutic results in chronic osteomyelitis.

  20. Clinical application and histological properties of autologous tissue-engineered skin equivalents using an acellular dermal matrix.

    Science.gov (United States)

    Takami, Yoshihiro; Yamaguchi, Ryo; Ono, Shimpei; Hyakusoku, Hiko

    2014-01-01

    We developed a transplantable tissue-engineered skin equivalent composed of autologous cultured keratinocytes, fibroblasts, and a decellularized allogeneic dermis (acellular allogeneic dermal matrix; ADM) obtained from cadavers. In a process taking 3 weeks, cultured autologous keratinocytes from burn patients were expanded and then grown on ADMs. The tissue-engineered autologous skin equivalents (TESEs) were then transplanted in a one-stage procedure to the debrided third-degree burn wounds of 4 patients. The mean graft survival rate was 96%. Delayed graft loss and graft fragility were not observed. Histological and immunohistological findings indicated that the transplanted TESE had similar characteristics to normal human split-thickness skin grafts. These results suggest that the TESE using ADM can be used for permanent repair of full-thickness skin defects.

  1. Similar effect of autologous and allogeneic cell therapy for ischemic heart disease : Systematic review and meta-analysis of large animal studies

    NARCIS (Netherlands)

    Jansen of Lorkeers, Sanne J.; Eding, Joep Egbert Coenraad; Vesterinen, Hanna Mikaela; van der Spoel, Tycho Ids Gijsbert; Sena, Emily Shamiso; Duckers, Henricus Johannes; Doevendans, Pieter Adrianus; Macleod, Malcolm Robert; Chamuleau, Steven Anton Jozef

    2015-01-01

    Rationale: In regenerative therapy for ischemic heart disease, use of both autologous and allogeneic stem cells has been investigated. Autologous cell can be applied without immunosuppression, but availability is restricted, and cells have been exposed to risk factors and aging. Allogeneic cell

  2. HLA class-II-restricted Mycobacterium leprae-reactive T-cell clones from leprosy patients established with a minimal requirement for autologous mononuclear cells

    NARCIS (Netherlands)

    Haanen, J. B.; Ottenhoff, T. H.; Voordouw, A.; Elferink, B. G.; Klatser, P. R.; Spits, H.; de Vries, R. R.

    1986-01-01

    This report describes an effective method for the cloning of Mycobacterium leprae-reactive T lymphocytes with Epstein-Barr-virus transformed autologous B cells as antigen-presenting cells. The two advantages of this method are that it drastically reduces the number of autologous peripheral blood

  3. Activated autologous T cells exert an anti-B-cell chronic lymphatic leukemia effect in vitro and in vivo.

    Science.gov (United States)

    Di Ianni, Mauro; Moretti, Lorenzo; Terenzi, Adelmo; Bazzucchi, Federico; Del Papa, Beatrice; Bazzucchi, Moira; Ciurnelli, Raffaella; Lucchesi, Alessandro; Sportoletti, Paolo; Rosati, Emanuela; Marconi, Pier Francesco; Falzetti, Franca; Tabilio, Antonio

    2009-01-01

    The impact of chronic lymphatic leukemia (CLL) tumor burden on the autologous immune system has already been demonstrated. This study attempted to elucidate the molecular mechanisms underlying T-cell immunologic deficiencies in CLL. Freshly isolated CD3(+) T cells from patients with a diagnosis of CLL and healthy donors were analyzed by gene expression profiling. Activated T cells from 20 patients with CLL were tested in vitro for cytotoxicity against mutated and unmutated autologous B cells and DAUDI, K562 and P815 cell lines. To investigate T-cell mediated cytotoxicity in vivo, we co-transplanted OKT3-activated T lymphocytes and autologous B-cell CLL (B-CLL) cells into NOD/SCID mice. Gene expression profiles of peripheral blood T cells from B-CLL patients showed 25 down-regulated, and 31 up-regulated, genes that were mainly involved in cell differentiation, proliferation, survival, apoptosis, cytoskeleton formation, vesicle trafficking and T-cell activation. After culture, the T-cell count remained unchanged, CD8 cells expanded more than CD4 and a cytotoxicity index >30% was present in 5/20 patients. Cytotoxicity against B autologous leukemic cells did not correlate with B-cell mutational status. Only activated T cells exerting cytotoxicity against autologous leukemic B cells prevented CLL in a human-mouse chimera. This study indicates that patients with CLL are affected by a partial immunologic defect that might be somewhat susceptible to repair. This study identifies the molecular pathways underlying T-cell deficiencies in CLL and shows that cytotoxic T-cell functions against autologous B-CLL can be rebuilt at least in part in vitro and in vivo.

  4. Comparison of Outcomes with Tissue Expander, Immediate Implant, and Autologous Breast Reconstruction in Greater Than 1000 Nipple-Sparing Mastectomies.

    Science.gov (United States)

    Frey, Jordan D; Choi, Mihye; Salibian, Ara A; Karp, Nolan S

    2017-06-01

    Nipple-sparing mastectomy permits complete preservation of the nipple-areola complex with excellent aesthetic results and with oncologic safety similar to that associated with traditional mastectomy techniques. However, outcomes have not been directly compared for tissue expander-, immediate implant-, and autologous tissue-based breast reconstruction after nipple-sparing mastectomy. All patients undergoing nipple-sparing mastectomy from 2006 to June of 2016 were identified at a single institution. Demographics and outcomes were analyzed and compared among different types of breast reconstruction. A total of 1028 nipple-sparing mastectomies were performed. Of these, 533 (51.8 percent) were tissue expander-based, 263 (25.6 percent) were autologous tissue-based, and 232 (22.6 percent) were immediate implant-based reconstructions. Tissue expander-based reconstructions had significantly more minor cellulitis (p = 0.0002) but less complete nipple necrosis (p = 0.0126) and major mastectomy flap necrosis (p < 0.0001) compared with autologous tissue-based reconstructions. Compared to immediate implant-based reconstruction, tissue expander-based reconstructions had significantly more minor cellulitis (p = 0.0006) but less complete nipple necrosis (p = 0.0005) and major (p < 0.0001) and minor (p = 0.0028) mastectomy flap necrosis (p = 0.0059). Immediate implant-based reconstructions had significantly more minor cellulitis (p = 0.0051), minor mastectomy flap necrosis (p = 0.0425), and partial nipple necrosis (p = 0.0437) compared with autologous tissue-based reconstructions. Outcomes were otherwise equivalent among the three groups. Tissue expander, immediate implant, and autologous tissue breast reconstruction techniques may all be safely offered with nipple-sparing mastectomy. However, reconstructive complications appear to be greater with immediate implant- and autologous tissue-based techniques compared with tissue expander-based reconstruction. Therapeutic, III.

  5. Effect of BCNU combined with total body irradiation or cyclophosphamide on survival of dogs after autologous marrow grafts

    International Nuclear Information System (INIS)

    Paterson, A.H.G.; English, D.

    1979-01-01

    Dogs were treated with either: (1) 750 rad total body irradiation; (2) BCNU 2 or 4 mg/kg IV 48 hours prior to 750 rad total body irradiation; or (3) BCNU 4 mg/kg IV plus cyclophosphamide 30 mg/kg IV. Results showed that of 11 dogs who received 750 rad total body irradiation and did not receive cryopreserved autologous bone marrow cells, none survived, compared to an 88% survival (31 of 35 dogs) after 750 rad total body irradiation if the dogs received stored autologous bone marrow cells. However, when the dogs were treated with BCNU 2 or 4 mg/kg prior to 750 rad total body irradiation the survival rate, despite infusion of autologous bone marrow cells, dropped to 25% (3 of 12 dogs) for BCNU 2 mg/kg, and 17% (2 of 12 dogs) for BCNU 4 mg/kg. This effect did not seem to be due to direct serum inhibition of hemopoietic cell proliferation since serum obtained at various intervals after BCNU administrations failed to inhibit CFU growth in vitro. The dogs died from hemorrhage and infection; at autopsy there was hemorrhagic pneumonitis and intestinal ulcerations with petechial hemorrhages, suggesting that the combination of BCNU and total body irradiation may have synergistic toxicity on the canine gastro-intestinal tract. When BCNU was combined with cyclophosphamide, reversal of marrow toxicity occurred in 54% (6 of 11 dogs) with stored autologous bone marrow cells compared to no survival (0 of 8 dogs) with stored autologous bone marrow cells. Thus while autologous bone marrow grafts are useful for reversal of marrow toxicity due to many therapeutic protocols, such grafts alone may not provide protection against toxicity due to the combination of high dosage BCNU and total body irradiation

  6. The Use of Autologous Fat Grafting for Treatment of Scar Tissue and Scar-Related Conditions: A Systematic Review.

    Science.gov (United States)

    Negenborn, Vera L; Groen, Jan-Willem; Smit, Jan Maerten; Niessen, Frank B; Mullender, Margriet G

    2016-01-01

    Scar tissue can cause cosmetic impairments, functional limitations, pain, and itch. It may also cause emotional, social, and behavioral problems, especially when it is located in exposed areas. To date, no gold standard exists for the treatment of scar tissue. Autologous fat grafting has been introduced as a promising treatment option for scar tissue-related symptoms. However, the scientific evidence for its effectiveness remains unclear. This systematic review aims to evaluate the available evidence regarding the effectiveness of autologous fat grafting for the treatment of scar tissue and scar-related conditions. A systematic literature review was performed using MEDLINE, Cochrane Library, EMBASE, and Web of Science. No language restrictions were imposed. Twenty-six clinical articles were included, reporting on 905 patients in total. Meta-analysis was not performed because of the heterogeneous methodology demonstrated among the articles. Main outcome measures were scar appearance and skin characteristics, restoration of volume and/or (three-dimensional) contour, itch, and pain. All publications report a beneficial effect of autologous fat grafting on scar tissue. There is statistical significant improvement of the scar appearance, skin characteristics, and pain. Itch and restoration of volume and three-dimensional contour also improved. Autologous fat grafting is used to improve a variety of symptoms related to scar tissue. This systematic review suggests that autologous fat grafting provides beneficial effects with limited side effects. However, the level of evidence and methodological quality are quite low. Future randomized controlled trials with a methodologically strong design are necessary to confirm the effects of autologous fat grafting on scar tissue and scar-related conditions.

  7. T-cell-replete haploidentical transplantation versus autologous stem cell transplantation in adult acute leukemia: a matched pair analysis

    Science.gov (United States)

    Gorin, Norbert-Claude; Labopin, Myriam; Piemontese, Simona; Arcese, William; Santarone, Stella; Huang, He; Meloni, Giovanna; Ferrara, Felicetto; Beelen, Dietrich; Sanz, Miguel; Bacigalupo, Andrea; Ciceri, Fabio; Mailhol, Audrey; Nagler, Arnon; Mohty, Mohamad

    2015-01-01

    Adult patients with acute leukemia in need of a transplant but without a genoidentical donor are usually considered upfront for transplantation with stem cells from any other allogeneic source, rather than autologous stem cell transplantation. We used data from the European Society for Blood and Marrow Transplantation and performed a matched pair analysis on 188 T-cell-replete haploidentical and 356 autologous transplants done from January 2007 to December 2012, using age, diagnosis, disease status, cytogenetics, and interval from diagnosis to transplant as matching factors. “Haploidentical expert” centers were defined as having reported more than five haploidentical transplants for acute leukemia (median value for the study period). The median follow-up was 28 months. Multivariate analyses, including type of transplant categorized into three classes (“haploidentical regular”, “haploidentical expert” and autologous), conditioning intensity (reduced intensity versus myeloablative conditioning) and the random effect taking into account associations related to matching, showed that non-relapse mortality was higher following haploidentical transplants in expert (HR: 4.7; P=0.00004) and regular (HR: 8.98; Ptransplants was lower in expert centers (HR:0.39; P=0.0003) but in regular centers was similar to that for autologous transplants. Leukemia-free survival and overall survival rates were higher following autologous transplantation than haploidentical transplants in regular centers (HR: 1.63; P=0.008 and HR: 2.31; P=0.0002 respectively) but similar to those following haploidentical transplants in expert centers. We conclude that autologous stem cell transplantation should presently be considered as a possible alternative to haploidentical transplantation in regular centers that have not developed a specific expert program. PMID:25637051

  8. Oral mucosa: an alternative epidermic cell source to develop autologous dermal-epidermal substitutes from diabetic subjects

    Science.gov (United States)

    GUZMÁN-URIBE, Daniela; ALVARADO-ESTRADA, Keila Neri; PIERDANT-PÉREZ, Mauricio; TORRES-ÁLVAREZ, Bertha; SÁNCHEZ-AGUILAR, Jesus Martin; ROSALES-IBÁÑEZ, Raúl

    2017-01-01

    Abstract Oral mucosa has been highlighted as a suitable source of epidermal cells due to its intrinsic characteristics such as its higher proliferation rate and its obtainability. Diabetic ulcers have a worldwide prevalence that is variable (1%-11%), meanwhile treatment of this has been proven ineffective. Tissue-engineered skin plays an important role in wound care focusing on strategies such autologous dermal-epidermal substitutes. Objective The aim of this study was to obtain autologous dermal-epidermal skin substitutes from oral mucosa from diabetic subjects as a first step towards a possible clinical application for cases of diabetic foot. Material and Methods Oral mucosa was obtained from diabetic and healthy subjects (n=20 per group). Epidermal cells were isolated and cultured using autologous fibrin to develop dermal-epidermal in vitro substitutes by the air-liquid technique with autologous human serum as a supplement media. Substitutes were immunocharacterized with collagen IV and cytokeratin 5-14 as specific markers. A Student´s t- test was performed to assess the differences between both groups. Results It was possible to isolate epidermal cells from the oral mucosa of diabetic and healthy subjects and develop autologous dermal-epidermal skin substitutes using autologous serum as a supplement. Differences in the expression of specific markers were observed and the cytokeratin 5-14 expression was lower in the diabetic substitutes, and the collagen IV expression was higher in the diabetic substitutes when compared with the healthy group, showing a significant difference. Conclusion Cells from oral mucosa could be an alternative and less invasive source for skin substitutes and wound healing. A difference in collagen production of diabetic cells suggests diabetic substitutes could improve diabetic wound healing. More research is needed to determine the crosstalk between components of these skin substitutes and damaged tissues. PMID:28403359

  9. Oral mucosa: an alternative epidermic cell source to develop autologous dermal-epidermal substitutes from diabetic subjects

    Directory of Open Access Journals (Sweden)

    Daniela GUZMÁN-URIBE

    Full Text Available Abstract Oral mucosa has been highlighted as a suitable source of epidermal cells due to its intrinsic characteristics such as its higher proliferation rate and its obtainability. Diabetic ulcers have a worldwide prevalence that is variable (1%-11%, meanwhile treatment of this has been proven ineffective. Tissue-engineered skin plays an important role in wound care focusing on strategies such autologous dermal-epidermal substitutes. Objective The aim of this study was to obtain autologous dermal-epidermal skin substitutes from oral mucosa from diabetic subjects as a first step towards a possible clinical application for cases of diabetic foot. Material and Methods Oral mucosa was obtained from diabetic and healthy subjects (n=20 per group. Epidermal cells were isolated and cultured using autologous fibrin to develop dermal-epidermal in vitro substitutes by the air-liquid technique with autologous human serum as a supplement media. Substitutes were immunocharacterized with collagen IV and cytokeratin 5-14 as specific markers. A Student´s t- test was performed to assess the differences between both groups. Results It was possible to isolate epidermal cells from the oral mucosa of diabetic and healthy subjects and develop autologous dermal-epidermal skin substitutes using autologous serum as a supplement. Differences in the expression of specific markers were observed and the cytokeratin 5-14 expression was lower in the diabetic substitutes, and the collagen IV expression was higher in the diabetic substitutes when compared with the healthy group, showing a significant difference. Conclusion Cells from oral mucosa could be an alternative and less invasive source for skin substitutes and wound healing. A difference in collagen production of diabetic cells suggests diabetic substitutes could improve diabetic wound healing. More research is needed to determine the crosstalk between components of these skin substitutes and damaged tissues.

  10. A multi-center, randomized, clinical study to compare the effect and safety of autologous cultured osteoblast(Ossron™ injection to treat fractures

    Directory of Open Access Journals (Sweden)

    Jeon Taek-Soo

    2009-02-01

    Full Text Available Abstract Background We performed a multicenter, open, randomized, clinical study of autologous cultured osteoblast injection for long-bone fracture, to evaluate the fracture healing acceleration effect and the safety of autologous cultured osteoblasts. Methods Sixty-four patients with long-bone fractures were randomly divided into two groups, i.e. those who received autologous cultured osteoblast injection and those who received no treatment. The sum of the difference in the callus formation scores after four and eight weeks, was used as the first efficacy variable. Results The autologous cultured osteoblast injection group showed fracture healing acceleration of statistical significance, and there were no specific patient complications when using this treatment. Conclusion Autologous cultured osteoblast injection should therefore be considered as a successful treatment option for treating long-bone fracture. Trial registration Current Controlled Trials ISRCTN10637905

  11. Clinical analysis of thoracoscopic surgery combined with intraoperative autologous blood transfusion in the treatment of traumatic hemothorax

    Directory of Open Access Journals (Sweden)

    Hu-Sai Ma

    2016-12-01

    Full Text Available From January 2013 to January 2015, 19 patients of traumatic hemothorax with hemorrhagic shock were treated in our department by thoracoscopic surgery combined with autologous blood transfusion. This study retrospectively analyzed the therapeutic effect and shared our experience. The average amount of blood transfused back was 662.41 ml ± 269.15 ml. None of the patients developed transfusion reaction and were all discharged uneventfully. Thoracoscopic surgery combined with autologous blood transfusion is effective in the rescue of patients with progressive hemothorax and hemorrhagic shock. When corresponding indications are well managed, treatment for these patients is quicker, safer, and more effective.

  12. Autologous HIV-1 neutralizing antibodies: emergence of neutralization-resistant escape virus and subsequent development of escape virus neutralizing antibodies

    DEFF Research Database (Denmark)

    Arendrup, M; Nielsen, C; Hansen, J E

    1992-01-01

    The capacity of consecutive human sera to neutralize sequentially obtained autologous virus isolates was studied. HIV-1 was isolated three times over a 48-164-week period from three individuals immediately after seroconversion and from two individuals in later stages of infection. Development...... escape virus may be part of the explanation of the apparent failure of the immune system to control HIV infection....... of neutralizing antibodies to the primary virus isolates was detected 13-45 weeks after seroconversion. Emergence of escape virus with reduced sensitivity to neutralization by autologous sera was demonstrated. The patients subsequently developed neutralizing antibodies against the escape virus but after a delay...

  13. Early Prognostic Value of Monitoring Serum Free Light Chain in Patients with Multiple Myeloma Undergoing Autologous Stem Cell Transplantation.

    Science.gov (United States)

    Özkurt, Zübeyde Nur; Sucak, Gülsan Türköz; Akı, Şahika Zeynep; Yağcı, Münci; Haznedar, Rauf

    2017-03-16

    We hypothesized the levels of free light chains obtained before and after autologous stem cell transplantation can be useful in predicting transplantation outcome. We analyzed 70 multiple myeloma patients. Abnormal free light chain ratios before stem cell transplantation were found to be associated early progression, although without any impact on overall survival. At day +30, the normalization of levels of involved free light chain related with early progression. According to these results almost one-third reduction of free light chain levels can predict favorable prognosis after autologous stem cell transplantation.

  14. [Manfred Schneider. Lübeck im 12. und 13. Jahrhundert. Archäologische Befunde zur entstehung einer mittelalterlichen Grossstadt. In : Expansion - integration? Danish-Baltic Contacts 1147-1410] / Dennis Hortmuth

    Index Scriptorium Estoniae

    Hortmuth, Dennis

    2011-01-01

    Arvustus:Manfred Schneider. Lübeck im 12. und 13. Jahrhundert. Archäologische Befunde zur entstehung einer mittelalterlichen Grossstadt. In : Expansion - integration? Danish-Baltic Contacts 1147-1410 AD. (Vordingborg, 2009).

  15. [Thomas Ditt: „Stoßtruppfakultät Breslau“. Rechtswissenschaft im „Grenzland Schlesien“ 1933-1945. (Beiträge zur Rechtsgeschichte des 20. Jahrhunderts ; 67). Mohr Siebeck. Tübingen 2011] / Marju Luts-Sootak

    Index Scriptorium Estoniae

    Luts-Sootak, Marju, 1966-

    2013-01-01

    Arvustus: Thomas Ditt. „Stoßtruppfakultät Breslau“. Rechtswissenschaft im „Grenzland Schlesien“ 1933-1945. (Beiträge zur Rechtsgeschichte des 20. Jahrhunderts, Bd. 67.) Mohr Siebeck. Tübingen 2011

  16. Cumulative energy requirement for manufacturing energy stores; Kumulierter Energieaufwand zur Herstellung von Energiespeichern

    Energy Technology Data Exchange (ETDEWEB)

    Schedl, T.

    1994-12-31

    The stores dealt with are exclusively stores for electrical supply systems. The use of stores for electrical energy should optimize the use of primary energy for electricity generation, but also the store itself. As a first step, the cumulative energy requirement for the manufacture (KEA{sub H}) of electro-chemical stores, superconducting magnetic field stores (SMES), flywheel stores and pumped stores are determined. Starting from a common balance limit, a comparison is made with regard to the ratio of discharge energy/KEA{sub H}. The actual store represents the balance limit. Only those components of all stores, which guarantee readiness for use, are considered. For the battery store this is the whole battery, including electrodes, electrolyte etc and the case. Regarding the SMES, this includes the superconductor, the vacuum tank, the cooling plant and measures to take up the mechanical forces. Regarding the flywheel store, this is the synchronous machine with the rotating mass and the vacuum tank. The pumped store is divided into building measures and the sets of machines. These consist of the turbines, snychronous machines and pumps. (orig./HW) [Deutsch] Bei den behandelten Speichern handelt es sich ausschliesslich um Speicher fuer elektrische Versorgungssysteme. Der Einsatz von Speichern fuer elektrische Energie soll den Primaerenergieeinsatz der Stromerzeugung, aber auch der Speicher selbst optimieren. Als erster Schritt wird der Kumulierte Energieaufwand zur Herstellung (KEA{sub H}) von elektrochemischen Speichern, Supraleitenden Magnetfeldspeichern (SMES), Schwungradspeichern und Pumpspeichern bestimmt. Ausgehend von einer gemeinsamen Bilanzgrenze wird hinsichtlich des Verhaeltnisses Entladeenergie/KEA{sub H} ein Quervergleich vorgenommen. Die Bilanzgrenze stellt der eigentliche Speicher dar. Es werden fuer alle Speicher nur die Komponenten betrachtet, welche die Einsatzbereitschaft gewaehrleisten. Beim Batteriespeicher ist dies die gesamte Batterie, incl

  17. [Rolf Hammel-Kiesow. Der Lübecker Katasterplan des 19. Jahrhunderts als historische Quelle. Überlegungen zur Stadtentwicklung Lübecks aus archäologischer, historischer und bauhistorischen Zicht] / Dennis Hormuth

    Index Scriptorium Estoniae

    Hormuth, Dennis

    2013-01-01

    Arvustus : Rolf Hammel-Kiesow. Der LÜbecker Katasterplan des 19. Jahrhunderts als historische Quelle. Überlegungen zur Stadtentwicklung Lübecks aus archäologischer, historischer und bauhistorischen Sicht. In: Stadtgründung und Stadterweiterung. Beiträge von Archäologie und Stadtgeschichtsforschung (=Beiträge zur Geschichte der Städte Mitteleuropas XXII). Linz, 2011. S. 75-104

  18. Regensburger Beiträge zur Bodenkunde, Landschaftsökologie und Quartärforschung; Band 12: Kolluvien und Auensedimente als Geoarchive im Umfeld der historischen Hammerwerke Leidersdorf und Wolfsbach (Vils/Opf.)

    OpenAIRE

    Beckmann, Sabine

    2007-01-01

    Die vorliegende Studie leistet einen Beitrag zur Erfassung der bergbaubedingten Folgen auf die Landschaft der Oberpfalz. Der ostbayerische Raum besitzt eine jahrhundertelange Tradition im Abbau und der Verarbeitung von Erzen. Südlich von Amberg wurden an der Vils zahlreiche Hammerwerke zur Verhüttung und Verarbeitung von Eisenerz errichtet. Dies ist verbunden mit flussbaulichen Maßnahmen und Stoffeinträgen in die Aue. Hauptziel der Arbeit ist es, die anthropogene Beeinflussung der Sedimente d...

  19. Efficacy of Autologous Microfat Graft on Facial Handicap in Systemic Sclerosis Patients.

    Science.gov (United States)

    Sautereau, Nolwenn; Daumas, Aurélie; Truillet, Romain; Jouve, Elisabeth; Magalon, Jéremy; Veran, Julie; Casanova, Dominique; Frances, Yves; Magalon, Guy; Granel, Brigitte

    2016-03-01

    Autologous adipose tissue injection is used in plastic surgery for correction of localized tissue atrophy and has also been successfully offered for treatment of localized scleroderma. We aimed to evaluate whether patients with systemic sclerosis (SSc) and facial handicap could also benefit from this therapy. We included 14 patients (mean age of 53.8 ± 9.6 years) suffering from SSc with facial handicap defined by Mouth Handicap in Systemic Sclerosis Scale (MHISS) score more than or equal to 20, a Rodnan skin score on the face more than or equal to 1, and maximal mouth opening of less than 55 mm. Autologous adipose tissue injection was performed under local anesthesia using the technique of subcutaneous microinjection. The main objective of this study was an improvement of the MHISS score 6 months after the surgical treatment. The procedure was well tolerated. We observed a mean decrease in the MHISS score of 10.7 points (±5.1; P handicap, skin sclerosis, mouth opening limitation, sicca syndrome, and facial pain. Thus, this minimally invasive approach offers a new hope for face therapy for patients with SSc.

  20. Correction of facial lipoatrophy using autologous fat transplants in HIV-infected adolescents.

    Science.gov (United States)

    Dollfus, C; Blanche, S; Trocme, N; Funck-Brentano, I; Bonnet, F; Levan, P

    2009-05-01

    Antiretroviral therapy has dramatically improved the survival of HIV-infected children. Nevertheless, side effects comparable to those found in adults have been encountered, such as facial lipoatrophy, which can have a negative impact on the self-esteem of otherwise healthy adolescents. Cosmetic surgical procedures in adolescents raise psychological issues which need to be specifically addressed and which have never been previously reported in this population. We evaluated the patient satisfaction, safety and cosmetic results of HIV-infected adolescents who experienced autologous fat transplants for the correction of facial lipoatrophy. We report the results of plastic surgery using autologous fat transplants (Coleman's Lipostructure) in six HIV-infected adolescents with facial lipoatrophy: three boys and three girls, aged 14-19 years. The quantity of reinjected fat on each side of the face varied from 5 to 12 mL within a single procedure. All the patients reported being satisfied or very satisfied with the cosmetic results and reported a positive impact on their daily life. With well-trained surgeons and carefully selected indications, corrective surgery of facial lipoatrophy in HIV-infected adolescents can provide immediate and long-lasting benefits in terms of physical appearance and psychological wellbeing, and should be considered as a component of comprehensive care.

  1. Women's Experiences With Flap Failure After Autologous Breast Reconstruction: A Qualitative Analysis.

    Science.gov (United States)

    Higgins, Kristen S; Gillis, Joshua; Williams, Jason G; LeBlanc, Martin; Bezuhly, Michael; Chorney, Jill M

    2017-05-01

    Clinical experience suggests that flap failure after autologous breast reconstruction can be a devastating experience for women. Previous research has examined women's experiences with autologous breast reconstruction with and without complications, and patients' experiences with suboptimal outcomes from other medical procedures. The authors aimed to examine the psychosocial experience of flap failure from the patient's perspective. Seven women who had experienced unilateral flap failure after deep inferior epigastric perforator flap surgery in the past 12 years completed semistructured interviews about their breast cancer treatments, their experiences with flap failure, the impact of flap failure on their lives, and the coping strategies they used. Interpretive phenomenological analysis, a type of qualitative analysis that provides an in-depth account of participant's experiences and their meanings, was used to analyze the interview data. From these data, patient-derived recommendations were developed for surgeons caring for women who have experienced flap failure. Three main themes (6 subthemes) emerged: coming to terms with flap failure (coping with emotions, body dissatisfaction); making meaning of flap failure experience (questioning, relationship with surgeon); and care providers acknowledging the emotional experience of flap failure (experience of being treated "mechanically," suggestions for improvement). In conclusion, flap failure in breast reconstruction is an emotionally difficult experience for women. Although there are similarities to other populations of patients experiencing suboptimal outcomes from medical procedures, there are also unique aspects of the flap failure experience. A better understanding of women's experiences with flap failure will assist in providing more appropriate supports.

  2. The effect of autologous platelet rich plasma in treatment lateral epicondylitis

    Directory of Open Access Journals (Sweden)

    İsmail Ağır

    2011-03-01

    Full Text Available Lateral epicondylitis (tennis elbow is the most commonly diagnosed cause of lateral elbow pain. The aim of this study was to compare the effect of single dose corticosteroid and autologous platelet rich plasma (PRP injection in the treatment of tennis elbow.Materials and methods: The 15 elbow of 15 patients (6 male and 9 female was included in the study, who applied to our clinic with lateral elbow pain and diagnosed as lateral epicondylitis. A single dose of 0,5 ml Bethametasone and 0,5 ml Prilocaine mixture was applied to first group and single dose 1 ml autologous PRP was locally applied to the second group.Results: In the early follow-ups the results of corticosteroid group were better than latter follow-ups, however in PRP group the results were worst in early follow-ups but better results were obtained in later follow-ups according to Verhaar scoring system.Conclusion: According to our results, the beneficial effects of PRP injection in lateral epicondylitis increases over time but further studies with more patients and longer follow up durations should done in order to more clarified this subject.

  3. Autologous platelet-rich plasma: a potential therapeutic tool for promoting hair growth.

    Science.gov (United States)

    Li, Zheng Jun; Choi, Hye-In; Choi, Dae-Kyoung; Sohn, Kyung-Cheol; Im, Myung; Seo, Young-Joon; Lee, Young-Ho; Lee, Jeung-Hoon; Lee, Young

    2012-07-01

    Recently, autologous platelet-rich plasma (PRP) has attracted attention in various medical fields, including plastic and orthopedic surgery and dermatology, for its ability to promote wound healing. PRP has been tested during facelift and hair transplantation to reduce swelling and pain and to increase hair density. To investigate the effects of PRP on hair growth using in vivo and in vitro models. PRP was prepared using the double-spin method and applied to dermal papilla (DP) cells. The proliferative effect of activated PRP on DP cells was measured. To understand the mechanisms of activated PRP on hair growth, we evaluated signaling pathways. In an in vivo study, mice received subcutaneous injections of activated PRP, and their results were compared with control mice. Activated PRP increased the proliferation of DP cells and stimulated extracellular signal-regulated kinase (ERK) and Akt signaling. Fibroblast growth factor 7 (FGF-7) and beta-catenin, which are potent stimuli for hair growth, were upregulated in DP cells. The injection of mice with activated PRP induced faster telogen-to-anagen transition than was seen on control mice. Although few studies tested the effects of activated PRP on hair growth, this research provides support for possible clinical application of autologous PRP and its secretory factors for promotion of hair growth. © 2012 by the American Society for Dermatologic Surgery, Inc. Published by Wiley Periodicals, Inc.

  4. FRET microscopy autologous tumor lysate processing in mature dendritic cell vaccine therapy

    Directory of Open Access Journals (Sweden)

    Ridolfi Ruggero

    2010-06-01

    Full Text Available Abstract Background Antigen processing by dendritic cells (DC exposed to specific stimuli has been well characterized in biological studies. Nonetheless, the question of whether autologous whole tumor lysates (as used in clinical trials are similarly processed by these cells has not yet been resolved. Methods In this study, we examined the transfer of peptides from whole tumor lysates to major histocompatibility complex class II molecules (MHC II in mature dendritic cells (mDC from a patient with advanced melanoma. Tumor antigenic peptides-MHC II proximity was revealed by Förster Resonance Energy Transfer (FRET measurements, which effectively extends the application of fluorescence microscopy to the molecular level ( Results We detected significant energy transfer between donor and acceptor-labelled antibodies against HLA-DR at the membrane surface of mDC. FRET data indicated that fluorescent peptide-loaded MHC II molecules start to accumulate on mDC membranes at 16 hr from the maturation stimulus, steeply increasing at 22 hr with sustained higher FRET detected up to 46 hr. Conclusions The results obtained imply that the patient mDC correctly processed the tumor specific antigens and their display on the mDC surface may be effective for several days. These observations support the rationale for immunogenic efficacy of autologous tumor lysates.

  5. Autologous Bone Marrow Mononuclear Cell Transplantation Delays Progression of Carotid Atherosclerosis in Rabbits.

    Science.gov (United States)

    Cui, Kefei; Ma, Xiao; Yu, Lie; Jiang, Chao; Fu, Chao; Fu, Xiaojie; Yu, Xiaofang; Huang, Yuanjing; Hou, Suyun; Si, Caifeng; Chen, Zhengguang; Yu, Jing; Wan, Jieru; Wang, Jian

    2016-09-01

    Bone marrow mononuclear cells (BMMNCs) can counteract oxidative stress and inhibit the inflammatory response in focal ischemic stroke models. However, the effect of BMMNC transplantation on carotid atherosclerosis needs to be determined. The carotid atherosclerotic plaque model was established in New Zealand White rabbits by balloon injury and 8 weeks of high-fat diet. Rabbits were randomized to receive an intravenous injection of autologous bromodeoxyuridine (BrdU)-labeled BMMNCs or an equal volume of phosphate-buffered saline. Plaques were evaluated for expression of proinflammatory and anti-inflammatory cytokines, anti-oxidant proteins, and markers of cell death. BMMNCs migrated into atherosclerotic plaque on the first day after cell transplantation. BMMNC-treated rabbits had smaller plaques and more collagen deposition than did the vehicle-treated controls on day 28 (p Autologous BMMNC transplantation can suppress the process of atherosclerotic plaque formation and is associated with enhanced anti-oxidative effect, reduced levels of inflammatory cytokines and cleaved caspase-3, and increased expression of insulin-like growth factor-1 and its receptor. BMMNC transplantation represents a novel approach for the treatment of carotid atherosclerosis.

  6. UPDATE ON THE ROLE OF AUTOLOGOUS HEMATOPOIETIC STEM CELL TRANSPLANTATION IN MULTIPLE MYELOMA

    Directory of Open Access Journals (Sweden)

    Patrizia Tosi

    2012-01-01

    Full Text Available

    Autologous stem cell transplantation is considered the standard of care for multiple myeloma patients aged < 65 years with no relevant comorbidities. The addition of drugs acting both on bone marrow microenvironment and on neoplastic plasma cells has significantly increased the proportion of patients achieving a complete remission after induction therapy, and these results are mantained after high-dose melphalan, leading to a prolonged disease control. Studies are being carried out in order to evaluate whether short term consolidation or long-term maintenance therapy can result into disease eradication at the molecular level thus increasing also patients survival. The efficacy of these new drugs has raised the issue of deferring the transplant after achivng a second response upon relapse. Another controversial point is the optimal treatment strategy for high-risk patients, that do not benefit from autologous stem cell transplantation and for whom the efficacy of new drugs is still matter of debate.

  7. A Simple, Reliable, and Inexpensive Intraoperative External Expansion System for Enhanced Autologous Structural Fat Grafting.

    Science.gov (United States)

    Oranges, Carlo M; Tremp, Mathias; Ling, Barbara; Wettstein, Reto; Largo, René D; Schaefer, Dirk J

    2016-09-01

    External volume expansion of the recipient site by suction has been proposed as a way of improving fat graft survival. The objective of this study was to present an innovative and simple intraoperative external expansion system to enhance small-volume autologous fat grafting (40-80 mL) and to discuss its background and its mechanism of action. In this system, expansion is performed using a complete vacuum delivery system known as the Kiwi VAC-6000M with a PalmPump (Clinical Innovations). The recipient site is rapidly expanded intraoperatively 10 times for 30 seconds each with a negative pressure of up to 550 mm Hg before autologous fat injection. During this repetitive stimulation, the tissues become grossly expanded, developing macroscopic swelling that regresses slowly over the course of hours following the cessation of the stimulus. The system sets various mechanisms in motion, including scar release, mechanical stimulation, edema, ischemia, and inflammation, which provide an environment conducive for cell proliferation and angiogenesis. In order to maintain the graft construct in its expansive state, all patients are encouraged postoperatively to use the Kiwi three times daily for one minute per session over the course of three days. The handling of this system is simple for both the patients and the surgeon. Satisfactory clinical outcomes have been achieved without significant complications.

  8. Comparison of Puddu osteotomy with or without autologous bone grafting: a prospective clinical trial

    Directory of Open Access Journals (Sweden)

    Marcus Ceregatti Passarelli

    Full Text Available ABSTRACT Objectives: To test the hypothesis that autologous iliac bone grafts do not enhance clinical results and do not decrease complication rates in patients undergoing medial opening-wedge high tibial , osteotomy. Methods: Forty patients allocated in a randomized, two-armed, double-blinded clinical trial were evaluated between 2007 and 2010. One group received bone graft, and the other group was left without filling the osteotomy defect. The primary outcome was the Knee Society Score. , Radiographic measurement of the frontal anatomical femoral-tibial angle and the progression of osteoarthritis according to the modified Ahlback classification were used as secondary outcomes., Results: There was no difference in KSS scale between the graft group (64.4 ± 21.8 and the graftless group (61.6 ± 17.3; p= 0.309. There was no difference of angle between the femur and tibia in the frontal plane between the groups (graft, = 184 ± 4.6 degrees, graftless = 183.4 ± 5.1 degrees; p= 1.0, indicating that there is no loss of correction due to the lack of the graft. There was significant aggravation of osteoarthritis in a greater number of patients in a graft group (p= 0.005 . Conclusion: Autologous iliac bone graft does not improve clinical outcomes in medium and long-term follow-up of medial opening-wedge high tibial osteotomy fixed with a first generation Puddu plate in the conditions of this study.

  9. Evaluation of immediate breast reconstruction after breast-conserving therapy using autologous free dermal fat flap

    International Nuclear Information System (INIS)

    Kadoya, Takayuki; Nishisaka, Takashi; Mukai, Shoichiro; Saeki, Yoshihiro; Sakimoto, Hideto; Eto, Takaaki; Takahashi, Makoto

    2010-01-01

    We performed immediate breast reconstruction using autologous free dermal fat flap followed by irradiation to the breast in a total of 11 patients with breast cancer in whom breast-conserving therapy was difficult. Their postoperative courses were satisfactory and they had not experienced infection and necrosis of the flap Postoperative MRI examination confirmed that the blood flow had resumed only in the periphery of the flap, but no blood flow was seen in the center part of the flap and the size of the flap had become smaller than that when it had been collected. Core needle biopsy showed that fatty tissue of the flap had dropped while its tissue structure had been kept intact, of which histological features were compatible with those of coagulation and necrosis of the tissue due to decreased flap perfusion. The cosmetic evaluation of the reconstructed breast was rated as an average score of 10.0 according to the Sawai group's criteria of the Japanese Breast Cancer Society. The postoperative flap was felt hard in many cases, but good outcomes were gained in all cases in terms of the lowest hanging point of the breast, location of the nipple, and balance between the right and left breasts. Autologous free dermal fat flap is an easier and less invasive breast reconstruction method than myocutaneous flap and implant methods. It requires no positioning changes of the patient and can be performed not only by plastic surgeons but also by general surgeons. (author)

  10. Mastoid Obliteration with Autologous Bone in Mastoidectomy Canal Wall Down Surgery: a Literature Overview.

    Science.gov (United States)

    Alves, Ricardo Dourado; Cabral Junior, Francisco; Fonseca, Anna Carolina de Oliveira; Bento, Ricardo Ferreira

    2016-01-01

    Introduction The objectives of mastoidectomy in cholesteatoma are a disease-free and dry ear, the prevention of recurrent disease, and the maintenance of hearing or the possibility to reconstruct an affected hearing mechanism. Canal wall down mastoidectomy has been traditionally used to achieve those goals with greater or lesser degrees of success. However, canal wall down is an aggressive approach, as it involves creating an open cavity and changing the anatomy and physiology of the middle ear and mastoid. A canal wall up technique eliminates the need to destroy the middle ear and mastoid, but is associated with a higher rate of residual cholesteatoma. The obliteration technics arise as an effort to avoid the disadvantages of both techniques. Objectives Evaluate the effectiveness of the mastoid obliteration with autologous bone in mastoidectomy surgery with canal wall down for chronic otitis, with or without cholesteatoma. Data Synthesis We analyzed nine studies of case series comprehending similar surgery techniques on 1017 total cases of operated ears in both adults and children, with at least 12 months follow-up. Conclusion Mastoid Obliteration with autologous bone has been utilized for many years to present date, and it seems to be safe, low-cost, with low recurrence rates - similar to traditional canal wall down procedures and with greater water resistance and quality of life improvements.

  11. Reengineering autologous bone grafts with the stem cell activator WNT3A.

    Science.gov (United States)

    Jing, Wei; Smith, Andrew A; Liu, Bo; Li, Jingtao; Hunter, Daniel J; Dhamdhere, Girija; Salmon, Benjamin; Jiang, Jie; Cheng, Du; Johnson, Chelsey A; Chen, Serafine; Lee, Katherine; Singh, Gurpreet; Helms, Jill A

    2015-04-01

    Autologous bone grafting represents the standard of care for treating bone defects but this biomaterial is unreliable in older patients. The efficacy of an autograft can be traced back to multipotent stem cells residing within the bone graft. Aging attenuates the viability and function of these stem cells, leading to inconsistent rates of bony union. We show that age-related changes in autograft efficacy are caused by a loss in endogenous Wnt signaling. Blocking this endogenous Wnt signal using Dkk1 abrogates autograft efficacy whereas providing a Wnt signal in the form of liposome-reconstituted WNT3A protein (L-WNT3A) restores bone forming potential to autografts from aged animals. The bioengineered autograft exhibits significantly better survival in the hosting site. Mesenchymal and skeletal stem cell populations in the autograft are activated by L-WNT3A and mitotic activity and osteogenic differentiation are significantly enhanced. In a spinal fusion model, aged autografts treated with L-WNT3A demonstrate superior bone forming capacity compared to the standard of care. Thus, a brief incubation in L-WNT3A reliably improves autologous bone grafting efficacy, which has the potential to significantly improve patient care in the elderly. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. Human autologous mesenchymal stem cells with extracorporeal shock wave therapy for nonunion of long bones.

    Science.gov (United States)

    Zhai, Lei; Ma, Xin-Long; Jiang, Chuan; Zhang, Bo; Liu, Shui-Tao; Xing, Geng-Yan

    2016-09-01

    Currently, the available treatments for long bone nonunion (LBN) are removing of focus of infection, bone marrow transplantation as well as Ilizarov methods etc. Due to a high percentage of failures, the treatments are complex and debated. To develop an effective method for the treatment of LBN, we explored the use of human autologous bone mesenchymal stems cells (hBMSCs) along with extracorporeal shock wave therapy (ESWT). Sixty three patients of LBN were subjected to ESWT treatment and were divided into hBMSCs transplantation group (Group A, 32 cases) and simple ESWT treatment group (Group B, 31 cases). The patients were evaluated for 12 months after treatment. In Group A, 14 patients were healed and 13 showed an improvement, with fracture healing rate 84.4%. In Group B, eight patients were healed and 13 showed an improvement, with fracture healing rate 67.7%. The healing rates of the two groups exhibited a significant difference ( P 0.05). However, the callus formation in Group A was significantly higher than that in the Group B after treatment for 6, 9, and 12 months ( P Autologous bone mesenchymal stems cell transplantation with ESWT can effectively promote the healing of long bone nonunions.

  13. Predictors of Autologous Free Fat Graft Retention in the Management of Craniofacial Contour Deformities.

    Science.gov (United States)

    Denadai, Rafael; Raposo-Amaral, Cesar Augusto; Pinho, Andre Silveira; Lameiro, Thais Miguel; Buzzo, Celso Luiz; Raposo-Amaral, Cassio Eduardo

    2017-07-01

    Autologous free fat graft outcomes are not always predictable, and variables that can potentially influence fat graft retention are still not well understood or investigated. The purposes of this study were to assess fat graft retention in the management of craniofacial contour deformities and to identify possible predictive factors of this retention. A prospective analysis was conducted using consecutive patients with unilateral craniofacial contour deformities who underwent autologous free fat grafting between 2012 and 2015. Standardized ultrasonographic craniofacial soft-tissue thickness measurements were adopted to determine the fat graft retention. Bivariate and multivariate analyses were performed to identify independent predictors of 12-month postoperative fat graft retention. One hundred forty-two patients were enrolled. There was significant (all p 0.05) from 3 to 12 months postoperatively, with a 12-month fat graft retention rate of 67.7 percent. Age, Parry-Romberg syndrome, previous craniofacial bone surgery, grafted volume, and forehead unit were independently negative (all p < 0.05) predictors of fat graft retention, whereas cheek unit was an independently positive (all p < 0.05) predictor of retention. Craniofacial fat graft retention is achievable but remains somewhat unpredictable, with age, Parry-Romberg syndrome, previous bone surgical intervention, grafted volume, and recipient sites affecting retention. Risk, III.

  14. The Effect of Autologous Platelet Lysate Eye Drops: An In Vivo Confocal Microscopy Study

    Directory of Open Access Journals (Sweden)

    Antonio M. Fea

    2016-01-01

    Full Text Available Purpose. To determine the effectiveness of autologous platelet lysate (APL eye drops in patients with primary Sjögren syndrome (SS dry eye, refractory to standard therapy, in comparison with patients treated with artificial tears. We focused on the effect of APL on cornea morphology with the in vivo confocal microscopy (IVCM. Methods. Patients were assigned to two groups: group A used autologous platelet lysate QID, and group B used preservative-free artificial tears QID, for 90 days. Ophthalmological assessments included ocular surface disease index (OSDI, best corrected visual acuity (BCVA, Schirmer test, fluorescein score, and breakup time (BUT. A subgroup of patients in group A underwent IVCM: corneal basal epithelium, subbasal nerves, Langerhans cells, anterior stroma activated keratocytes, and reflectivity were evaluated. Results. 60 eyes of 30 patients were enrolled; in group A (n=20 patients mean OSDI, fluorescein score, and BUT showed significant improvement compared with group B (n=10 patients. The IVCM showed a significant increase in basal epithelium cells density and subbasal nerve plexus density and number and a decrease in Langerhans cells density (p<0.05. Conclusion. APL was found effective in the treatment of SS dry eye. IVCM seems to be a useful tool to visualize cornea morphologic modifications.

  15. Comparison of Two Apheresis Systems of COBE and Optia for Autologous Peripheral Blood Stem Cell Collection.

    Science.gov (United States)

    Lee, Se Na; Sohn, Ji Yeon; Kong, Jung Hee; Eom, Hyeon Seok; Lee, Hyewon; Kong, Sun Young

    2017-07-01

    Peripheral blood stem cell (PBSC) transplantation following myeloablative therapy is a mainstay of treatment for various types of malignancies. This study aimed to evaluate the differences between the Optia MNC and COBE Spectra MNC systems (Terumo BCT, Japan) according to apheresis procedures and the parameters of apheresis, products, and collection. The clinical data of 74 patients who underwent autologous PBSC collection from July 2012 to July 2015 were reviewed retrospectively. The patients comprised 48 (65%) men and 26 (35%) women with a median age of 56 yr (range, 23-66 yr). Of 216 procedures, 111 (51%) and 105 (49%) were processed by using COBE and Optia MNC, respectively. PBSC collection rates, throughput, numbers of stem cells retrieved, collection efficacy, and platelet loss were compared. There were no significant differences in the median CD34+ cell counts of collected products (0.61×10⁸ vs 0.94×10⁸), CD34 collection efficiency (43.5% vs 42.1%), and loss of platelets (40.1% vs 44.7%). The Spectra Optia MNC apheresis system was comparable to the COBE Spectra system in collecting autologous CD34+ hematopoietic stem cells and retention of platelets. © The Korean Society for Laboratory Medicine.

  16. Treatment of perforated cornea with an autologous lamellar scleral graft: histologic findings.

    Science.gov (United States)

    Jovanovic, Vesna; Jankov, Mirko; Nikolic, Ljubisa

    2018-01-01

    We report a case of central corneal perforation treated with an autologous lamellar scleral graft and histologic findings obtained after a subsequent penetrating keratoplasty. A corneal perforation within a large Pseudomonas ulcer in a 55-year-old male rigid gas permeable contact lens wearer was sealed by a lamellar scleral graft from the same eye, followed by an uneventful penetrating keratoplasty 6 months later. Histology of the excised button revealed that the well-apposed graft, which maintained the irregular arrangement of the scleral collagen fibers, was embedded in the corneal stroma over the deep blood vessels and a rupture in Descemet's membrane. The clinical and histologic findings showed that autologous lamellar scleral grafts can be successfully used for the emergency treatment of corneal perforation when a corneal transplant is not available. The distinctive scleral structure revealed by histology and the inadequate graft transparency indicate that visual rehabilitation of eyes with a central corneal perforation can be achieved only by a subsequent optic penetrating keratoplasty.

  17. Autologous mini punch grafting: an experience of using motorized power punch in 10 patients.

    Science.gov (United States)

    Chandrashekar, Bs; Madura, C; Varsha, Dv

    2014-01-01

    Autologous mini punch grafting (MPG) is a safe, effective and easy technique that can be performed on any site with minimal side effects and good cosmetic results. Large areas of stable generalised vitiligo require more grafts and are time consuming. Hence multiple sessions of surgery need to be scheduled. We share our experience of using motorised power punches to increase the speed of surgery in large areas of stable vitiligo in 10 patients. Ten patients in the age group of 12-55 years were treated with miniature punch grafting using power punches in single session on various sites. The power punches of 1-1.5 mm diameter were used to score donor and recipient sites, either of same or less than 0.2-0.3 mm size punches. The harvested grafts from donor site were then secured in the recipient beds and dressed. The average number of grafts harvested per session was 125-185, the duration of surgery ranged from 45 to 90 minutes. Perigraft pigment spread was seen at 3 weeks. Complete repigmentation was observed in 3-4 months in eight patients. Cobble stoning was observed in one patient, and all donor sites healed well with superficial scarring. We conclude that autologous MPG with motorised power punches for stable vitiligo, especially on large areas including difficult sites can be performed with ease in comparatively lesser time in a single session, greatly benefiting the patients.

  18. Autologous mini punch grafting: An experience of using motorized power punch in 10 patients

    Directory of Open Access Journals (Sweden)

    B S Chandrashekar

    2014-01-01

    Full Text Available Background: Autologous mini punch grafting (MPG is a safe, effective and easy technique that can be performed on any site with minimal side effects and good cosmetic results. Large areas of stable generalised vitiligo require more grafts and are time consuming. Hence multiple sessions of surgery need to be scheduled. We share our experience of using motorised power punches to increase the speed of surgery in large areas of stable vitiligo in 10 patients. Materials and Methods: Ten patients in the age group of 12-55 years were treated with miniature punch grafting using power punches in single session on various sites. The power punches of 1-1.5 mm diameter were used to score donor and recipient sites, either of same or less than 0.2-0.3 mm size punches. The harvested grafts from donor site were then secured in the recipient beds and dressed. Results: The average number of grafts harvested per session was 125-185, the duration of surgery ranged from 45 to 90 minutes. Perigraft pigment spread was seen at 3 weeks. Complete repigmentation was observed in 3-4 months in eight patients. Cobble stoning was observed in one patient, and all donor sites healed well with superficial scarring. Conclusion: We conclude that autologous MPG with motorised power punches for stable vitiligo, especially on large areas including difficult sites can be performed with ease in comparatively lesser time in a single session, greatly benefiting the patients.

  19. Cytomegalovirus viremia, viruria and disease after autologous peripheral blood stem cell transplantation: no need for surveillance.

    Science.gov (United States)

    Bilgrami, S; Aslanzadeh, J; Feingold, J M; Bona, R D; Clive, J; Dorsky, D; Edwards, R L; Tutschka, P J

    1999-07-01

    A retrospective evaluation of 200 consecutive recipients of autologous peripheral blood stem cell transplantation (PBSCT) was conducted to ascertain the incidence, risk factors, clinical features, complications, and outcome of cytomegalovirus (CMV) infection. A total of 26 patients (13%) developed CMV viremia (n = 5), DNAemia (n = 3), viruria (n = 18) and/or disease (n = 3) at a median of 45 days following stem cell infusion. None of the patients underwent surveillance testing for CMV. A diagnosis was established by culture and polymerase chain reaction of blood, urine or other tissue samples submitted when patients exhibited clinical features suggestive of CMV infection. Cytomegalovirus seropositivity prior to transplantation was the only statistically significant risk factor predicting subsequent identification of CMV (P < 0.001). The symptoms were severe enough in 23 patients to warrant treatment with intravenous ganciclovir. Three patients developed CMV disease; two developed fatal CMV pneumonia and one developed CMV gastritis which responded to antiviral treatment. Clinical signs and symptoms as well as viremia and viruria resolved with (20 patients) and without (three patients) treatment in the remaining individuals. All instances of CMV viremia, DNAemia, viruria and disease occurred within 3 months of stem cell infusion. These results demonstrate that CMV is a common pathogen after autologous PBSCT and may result in fatality in rare instances. Surveillance programs appear to be neither useful nor cost-effective. Diagnostic evaluation should be performed only in patients exhibiting suspicious clinical features and antiviral chemotherapy should be administered for persistent and severe signs and symptoms.

  20. Use of autologous grafts in the treatment of acquired penile curvature: An experience of 33 cases.

    Science.gov (United States)

    Khawaja, Abdul Rouf; Dar, Tanveer Iqbal; Zahur, Suhael; Tariq, Sheikh; Hamid, Arf; Wani, M S; Wazir, B S; Iqbal, Arsheed

    2016-01-01

    The objective was to compare the use of autologous dermal and temporalis fascia grafts in the treatment of acquired penile curvatures. It was a prospective observational study of 33 cases, conducted in Sher-i-Kashmir Institute of Medical Sciences, Srinagar from March 2007 to September 2013. All the patients had stable Peyronies disease (PD). Dorsal, dorsolateral and vental curvatures with good preoperative erections were included. PD index with visual analog scales for curvature was used preoperatively. An informed written consent was taken from all the patients with main emphasis on erectile dysfunction. After an average follow up of 2 years, complete straightening of penis was observed in all patients with satisfactory sexual intercourse in 30 patients (90%). Three patients (10%) required frequent use of type 5 phosphodiesterase inhibitors for adequate erections. Overall 91% of patients and partners were satisfied with the procedure and cosmetically donor site was better in temporalis fascia graft site. No rejection of any graft was noted and glans hypoesthesia was noticed in 4 patients (12%). None of the patients required penile prosthesis. Total operative time for harvesting and application of the graft was more in dermal grafts (>3 hrs) than for temporalis fascia graft (2 hrs). Tunical lengthening procedures by autologous free grafts represents a safe and reproducible technique. A good preoperative erectile function is required for tunical lengthening procedure. Temporalis fascia graft is thin, tough membrane and effective graft for PD with good cosmetic and functional results.