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Sample records for australian drosophila buzzatii

  1. Bottlenecks, population differentiation and apparent selection at microsatellite loci in Australian Drosophila buzzatii

    DEFF Research Database (Denmark)

    Barker, J.S.F.; Frydenberg, Jane; González, J.;

    2009-01-01

    variation for 15 microsatellite loci in each of nine populations in eastern Australia was used to estimate the size of the bottleneck, and to determine if any of these microsatellites marked genomic regions subject to recent selection. We estimate that on its introduction to Australia, D. buzzatii went...... through a moderate bottleneck (approximately 30-40 founders). Linkage disequilibrium was common, both intrachromosomal and between loci on different chromosomes. Of the 15 loci, 2 showed evidence of selection, one exhibiting local adaptation in different populations and the other balancing selection. We...

  2.     Developmental acclimation affects clinal variation in stress resistance traits in Drosophila buzzatii

    DEFF Research Database (Denmark)

    Sarup, Pernille Merete; Loeschcke, Volker

    2010-01-01

    -down resistance after development at either constant or fluctuating temperatures in nine Drosophila buzzatii populations collected along an altitudinal gradient in Tenerife, Spain. Flies that developed at fluctuating temperatures had higher stress resistance despite experiencing a slightly lower average...

  3. A BAC-based physical map of the Drosophila buzzatii genome

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez, Josefa; Nefedov, Michael; Bosdet, Ian; Casals, Ferran; Calvete, Oriol; Delprat, Alejandra; Shin, Heesun; Chiu, Readman; Mathewson, Carrie; Wye, Natasja; Hoskins, Roger A.; Schein, JacquelineE.; de Jong, Pieter; Ruiz, Alfredo

    2005-03-18

    Large-insert genomic libraries facilitate cloning of large genomic regions, allow the construction of clone-based physical maps and provide useful resources for sequencing entire genomes. Drosophilabuzzatii is a representative species of the repleta group in the Drosophila subgenus, which is being widely used as a model in studies of genome evolution, ecological adaptation and speciation. We constructed a Bacterial Artificial Chromosome (BAC) genomic library of D. buzzatii using the shuttle vector pTARBAC2.1. The library comprises 18,353 clones with an average insert size of 152 kb and a {approx}18X expected representation of the D. buzzatii euchromatic genome. We screened the entire library with six euchromatic gene probes and estimated the actual genome representation to be {approx}23X. In addition, we fingerprinted by restriction digestion and agarose gel electrophoresis a sample of 9,555 clones, and assembled them using Finger Printed Contigs (FPC) software and manual editing into 345 contigs (mean of 26 clones per contig) and 670singletons. Finally, we anchored 181 large contigs (containing 7,788clones) to the D. buzzatii salivary gland polytene chromosomes by in situ hybridization of 427 representative clones. The BAC library and a database with all the information regarding the high coverage BAC-based physical map described in this paper are available to the research community.

  4. Differences in wing melanization and pigmentation pattern in Drosophila buzzatii (Diptera: Drosophilidae under chemical stress

    Directory of Open Access Journals (Sweden)

    Nicolás MONGIARDINO KOCH

    2012-01-01

    Full Text Available Recientemente, hemos avanzado en nuestra comprensión de las bases genéticas y los mecanismos subyacentes al proceso de melanización durante el desarrollo, lo que permite el estudio de los procesos evolutivos que gobiernan la variabilidad de pigmentación presente en la naturaleza. Sin embargo, los factores ambientales que contribuyen a dicha variabilidad han recibido poca atención, aún cuando pueden conllevar profundas consecuencias a nivel ecológico. En este trabajo, presentamos un método para analizar los patrones de melanización alar de drosofílidos, de forma cualitativa y cuantitativa. Ponemos a prueba esta metodología comparando las alas de Drosophila buzzatii Patterson & Wheeler, criadas en medio control y con el agregado de alcaloides. Los alcaloides fueron extraídos del huésped secundario de estas moscas, Echinopsis terscheckii (Parm. ex Pfeiff. Friedrich & Rowley, los cuales, se sabe, presentan efectos negativos en el desarrollo de D. buzzatii. Las moscas criadas en un medio enriquecido con alcaloides mostraron una mayor melanización alar, junto con diferencias en la distribución espacial del pigmento. Planteamos modificaciones a nivel metabólico y de expresión génica para explicar los cambios que las condiciones de estrés inducen sobre el carácter. Proponemos la utilización de este procedimiento para el estudio de la dependencia ambiental de la melanización.

  5. Differences in wing melanization and pigmentation pattern in Drosophila buzzatii (Diptera: Drosophilidae under chemical stress Diferencias en la melanización y el patrón de pigmentación alar en Drosophila buzzatii (Diptera: Drosophilidae bajo estrés químico

    Directory of Open Access Journals (Sweden)

    Nicolás Mongiardino Koch

    2012-06-01

    Full Text Available Recently, the genetic basis and developmental mechanisms underlying the process of melanization have become progressively elucidated, allowing us to study the evolutionary processes that govern the huge variability of pigmentation observed in nature. However, environmental factors contributing to such variability have received little attention, even though they might have profound ecological consequences. Here we describe a method for analyzing the wing melanization patterns of drosophilids in both qualitative and quantitative ways. We test this method on wings of Drosophila buzzatii Patterson & Wheeler individuals, reared in control and alkaloid-enriched mediums. The alkaloids were extracted from the secondary host of these flies, Echinopsis terscheckii (Parm. ex Pfeiff. Friedrich & Rowley, and their effect on wing pigmentation is analyzed, bearing in mind the adverse effects that these compounds have on the development of D. buzzatii. Alkaloid-reared flies were shown to attain a higher degree of wing melanization, accompanied with differences in the spatial distribution of the pigment. Modifications at both metabolic and gene regulatory levels are proposed to explain the changes that chemical stressful conditions are shown to induce in this character. We propose the utilization of this procedure in studies of environmental dependence of pigmentation.Recientemente, hemos avanzado en nuestra comprensión de las bases genéticas y los mecanismos subyacentes al proceso de melanización durante el desarrollo, lo que permite el estudio de los procesos evolutivos que gobiernan la variabilidad de pigmentación presente en la naturaleza. Sin embargo, los factores ambientales que contribuyen a dicha variabilidad han recibido poca atención, aún cuando pueden conllevar profundas consecuencias a nivel ecológico. En este trabajo, presentamos un método para analizar los patrones de melanización alar de drosofílidos, de forma cualitativa y cuantitativa

  6. A collection of Australian Drosophila datasets on climate adaptation and species distributions.

    Science.gov (United States)

    Hangartner, Sandra B; Hoffmann, Ary A; Smith, Ailie; Griffin, Philippa C

    2015-11-24

    The Australian Drosophila Ecology and Evolution Resource (ADEER) collates Australian datasets on drosophilid flies, which are aimed at investigating questions around climate adaptation, species distribution limits and population genetics. Australian drosophilid species are diverse in climatic tolerance, geographic distribution and behaviour. Many species are restricted to the tropics, a few are temperate specialists, and some have broad distributions across climatic regions. Whereas some species show adaptability to climate changes through genetic and plastic changes, other species have limited adaptive capacity. This knowledge has been used to identify traits and genetic polymorphisms involved in climate change adaptation and build predictive models of responses to climate change. ADEER brings together 103 datasets from 39 studies published between 1982-2013 in a single online resource. All datasets can be downloaded freely in full, along with maps and other visualisations. These historical datasets are preserved for future studies, which will be especially useful for assessing climate-related changes over time.

  7. Dinámica evolutiva de las reordenaciones cromosómicas y coincidencia de los puntos de rotura: análisis molecular de las inversiones fijadas en el cromosoma 2 de Drosophila Buzzatii

    OpenAIRE

    Calvete Torres, Oriol

    2010-01-01

    El interés por los mecanismos que generan las reordenaciones cromosómicas se ha reavivado recientemente gracias al enorme avance que supone la comparación de genomas secuenciados. En Drosophila, las inversiones cromosómicas son abundantes como polimorfismos intraespecíficos y como cambios fijados entre especies. Sin embargo, aun sabemos poco acerca de la generación de estas inversiones naturales y sus consecuencias moleculares. Se conocen diversos mecanismos moleculares capaces de generar inv...

  8. The transposon Galileo generates natural chromosomal inversions in Drosophila by ectopic recombination.

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    Alejandra Delprat

    Full Text Available BACKGROUND: Transposable elements (TEs are responsible for the generation of chromosomal inversions in several groups of organisms. However, in Drosophila and other Dipterans, where inversions are abundant both as intraspecific polymorphisms and interspecific fixed differences, the evidence for a role of TEs is scarce. Previous work revealed that the transposon Galileo was involved in the generation of two polymorphic inversions of Drosophila buzzatii. METHODOLOGY/PRINCIPAL FINDINGS: To assess the impact of TEs in Drosophila chromosomal evolution and shed light on the mechanism involved, we isolated and sequenced the two breakpoints of another widespread polymorphic inversion from D. buzzatii, 2z(3. In the non inverted chromosome, the 2z(3 distal breakpoint was located between genes CG2046 and CG10326 whereas the proximal breakpoint lies between two novel genes that we have named Dlh and Mdp. In the inverted chromosome, the analysis of the breakpoint sequences revealed relatively large insertions (2,870-bp and 4,786-bp long including two copies of the transposon Galileo (subfamily Newton, one at each breakpoint, plus several other TEs. The two Galileo copies: (i are inserted in opposite orientation; (ii present exchanged target site duplications; and (iii are both chimeric. CONCLUSIONS/SIGNIFICANCE: Our observations provide the best evidence gathered so far for the role of TEs in the generation of Drosophila inversions. In addition, they show unequivocally that ectopic recombination is the causative mechanism. The fact that the three polymorphic D. buzzatii inversions investigated so far were generated by the same transposon family is remarkable and is conceivably due to Galileo's unusual structure and current (or recent transpositional activity.

  9. The Foldback-like element Galileo belongs to the P superfamily of DNA transposons and is widespread within the Drosophila genus.

    Science.gov (United States)

    Marzo, Mar; Puig, Marta; Ruiz, Alfredo

    2008-02-26

    Galileo is the only transposable element (TE) known to have generated natural chromosomal inversions in the genus Drosophila. It was discovered in Drosophila buzzatii and classified as a Foldback-like element because of its long, internally repetitive, terminal inverted repeats (TIRs) and lack of coding capacity. Here, we characterized a seemingly complete copy of Galileo from the D. buzzatii genome. It is 5,406 bp long, possesses 1,229-bp TIRs, and encodes a 912-aa transposase similar to those of the Drosophila melanogaster 1360 (Hoppel) and P elements. We also searched the recently available genome sequences of 12 Drosophila species for elements similar to Dbuz\\Galileo by using bioinformatic tools. Galileo was found in six species (ananassae, willistoni, peudoobscura, persimilis, virilis, and mojavensis) from the two main lineages within the Drosophila genus. Our observations place Galileo within the P superfamily of cut-and-paste transposons and extend considerably its phylogenetic distribution. The interspecific distribution of Galileo indicates an ancient presence in the genus, but the phylogenetic tree built with the transposase amino acid sequences contrasts significantly with that of the species, indicating lineage sorting and/or horizontal transfer events. Our results also suggest that Foldback-like elements such as Galileo may evolve from DNA-based transposon ancestors by loss of the transposase gene and disproportionate elongation of TIRs.

  10. Divergence at neutral and non-neutral loci in Drosophila buzzatii populations and their hybrids

    DEFF Research Database (Denmark)

    Andersen, Ditte Holm; Pertoldi, C.; Loeschcke, Volker;

    2008-01-01

    The impact of intraspecific hybridisation on fitness and morphological traits depends on the history of natural selection and genetic drift, which may have led to differently coadapted gene-complexes in the parental populations. The divergence at neutral and non-neutral loci between populations can...... be evaluated by estimating FST and QST respectively, and hence give an estimate of drift and selection in the populations. Here we investigate (1) whether divergence between populations in quantitative traits (wing size and shape) can be attributed to selection or drift alone, (2) The impact of intraspecific...

  11. Parallel effects of the inversion In(3R)Payne on body size across the North American and Australian clines in Drosophila melanogaster.

    Science.gov (United States)

    Kapun, M; Schmidt, C; Durmaz, E; Schmidt, P S; Flatt, T

    2016-05-01

    Chromosomal inversions are thought to play a major role in climatic adaptation. In D. melanogaster, the cosmopolitan inversion In(3R)Payne exhibits latitudinal clines on multiple continents. As many fitness traits show similar clines, it is tempting to hypothesize that In(3R)P underlies observed clinal patterns for some of these traits. In support of this idea, previous work in Australian populations has demonstrated that In(3R)P affects body size but not development time or cold resistance. However, similar data from other clines of this inversion are largely lacking; finding parallel effects of In(3R)P across multiple clines would considerably strengthen the case for clinal selection. Here, we have analysed the phenotypic effects of In(3R)P in populations originating from the endpoints of the latitudinal cline along the North American east coast. We measured development time, egg-to-adult survival, several size-related traits (femur and tibia length, wing area and shape), chill coma recovery, oxidative stress resistance and triglyceride content in homokaryon lines carrying In(3R)P or the standard arrangement. Our central finding is that the effects of In(3R)P along the North American cline match those observed in Australia: standard arrangement lines were larger than inverted lines, but the inversion did not influence development time or cold resistance. Similarly, In(3R)P did not affect egg-to-adult survival, oxidative stress resistance and lipid content. In(3R)P thus seems to specifically affect size traits in populations from both continents. This parallelism strongly suggests an adaptive pattern, whereby the inversion has captured alleles associated with growth regulation and clinal selection acts on size across both continents. PMID:26881839

  12. Genome-wide dissection of hybrid sterility in Drosophila confirms a polygenic threshold architecture.

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    Morán, Tomás; Fontdevila, Antonio

    2014-01-01

    To date, different studies about the genetic basis of hybrid male sterility (HMS), a postzygotic reproductive barrier thoroughly investigated using Drosophila species, have demonstrated that no single major gene can produce hybrid sterility without the cooperation of several genetic factors. Early work using hybrids between Drosophila koepferae (Dk) and Drosophila buzzatii (Db) was consistent with the idea that HMS requires the cooperation of several genetic factors, supporting a polygenic threshold (PT) model. Here we present a genome-wide mapping strategy to test the PT model, analyzing serially backcrossed fertile and sterile males in which the Dk genome was introgressed into the Db background. We identified 32 Dk-specific markers significantly associated with hybrid sterility. Our results demonstrate 1) a strong correlation between the number of segregated sterility markers and males' degree of sterility, 2) the exchangeability among markers, 3) their tendency to cluster into low-recombining chromosomal regions, and 4) the requirement for a minimum number (threshold) of markers to elicit sterility. Although our findings do not contradict a role for occasional major hybrid-sterility genes, they conform more to the view that HMS primarily evolves by the cumulative action of many interacting genes of minor effect in a complex PT architecture.

  13. Life extension and the position of the hormetic zone depends on sex and genetic background in Drosophila melanogaster

    DEFF Research Database (Denmark)

    Sarup, Pernille Merete; Loeschcke, Volker

    2011-01-01

    Hormesis, the beneficial effect of a mild stress, has been proposed as a means to prolong the period of healthy ageing as it can increase the average lifespan of a cohort. However, if we want to use hormesis therapeutically it is important that the treatment is beneficial on the individual level...... and not just on average at the population level. Long lived lines have been shown not to benefit from a, in other lines, hormesis inducing heat treatment in Drosophila melanogaster, D. buzzatii and mice. Also in many experiments hormesis has been reported to occur in one sex only, usually males but...... not in females. Here we investigated the interaction between the hormetic response and genetic background, sex and duration of a mild heat stress in D. melanogaster, using three replicate lines that have been selected for increased longevity and their respective control lines. We found that genetic...

  14. Genetic architecture of autosome-mediated hybrid male sterility in Drosophila

    Energy Technology Data Exchange (ETDEWEB)

    Marin, I. [Stanford Univ., CA (United States)

    1996-04-01

    Several estimators have been developed for assesing the number of sterility factors in a chromosome based on the sizes of fertile and sterile introgressed fragments. Assuming that two factors are required for producing sterility, simulations show that one of these, twice the inverse of the relative size of the largest fertile fragment, provides good average approximations when as few as five fertile fragments are analyzed. The estimators have been used for deducing the number of factors from previous data on several pairs of species. A particular result contrasts with the authors` interpretations: instead of the high number of sterility factors suggested, only a few per autosome are estimated in both reciprocal crosses involving Drosophila buzzatii and D. koepferae. It has been possible to map these factors, between three and six per chromosome, in the autosomes 3 and 4 of these species. Out of 203 introgressions of different fragments or combinations of fragments, the outcome of at least 192 is explained by the mapped zones. These results suggest that autosome-mediated sterility in the male hybrids of these species is mediated by a few epistatic factors, similarly to X-mediated sterility in the hybrids of other Drosophila species. 48 refs., 5 tabs.

  15. Australian Extinctions

    Science.gov (United States)

    Science Teacher, 2005

    2005-01-01

    Massive extinctions of animals and the arrival of the first humans in ancient Australia--which occurred 45,000 to 55,000 years ago--may be linked. Researchers at the Carnegie Institution, University of Colorado, Australian National University, and Bates College believe that massive fires set by the first humans may have altered the ecosystem of…

  16. Dicty_cDB: Contig-U10539-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .. 38 0.67 7 ( AY134811 ) Drosophila buzzatii chromosome 2 clone 60.29.1-ch... 40 0.73 2 ( AY134809 ) Drosophila buzz...B23, W... 42 0.93 3 ( AY134784 ) Drosophila buzzatii chromosome 2 clone 60.29.1-ch... 40 1.0 2 ( AY134804 ) Drosophila buzz...atii chromosome 2 clone 60.29.1-ch... 40 1.0 2 ( AY134790 ) Drosophila buzzatii chromosome 2 ...clone 60.29.1-ch... 40 1.0 2 ( AY134794 ) Drosophila buzzatii chromosome 2 clone ...60.29.1-ch... 40 1.0 2 ( AY134785 ) Drosophila buzzatii chromosome 2 clone 60.29.1-ch... 40 1.0 2 ( AY134808 ) Drosophila buzz

  17. Australian Research Council

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    @@ Introduction The Australian Research Council(ARC) is the Australian Government's main agency for allocating research funding to academics and researchers in Australian universities.Its mission is to deliver policy and programs that advance Australian research and innovation globally and benefit the community.

  18. Drosophila myogenesis.

    Science.gov (United States)

    Bothe, Ingo; Baylies, Mary K

    2016-09-12

    The skeletal muscle system is the largest organ in motile animals, constituting between 35 and 55% of the human body mass, and up to 75% of the body mass in flying organisms like Drosophila. The flight muscles alone in flying insects comprise up to 65% of total body mass. Not only is the musculature the largest organ system, it is also exquisitely complex, with single muscles existing in different shapes and sizes. These different morphologies allow for such different functions as the high-frequency beating of a wing in a hummingbird, the dilation of the pupil in a human eye, or the maintenance of posture in a giraffe's neck. PMID:27623256

  19. Variations on a theme: diversification of cuticular hydrocarbons in a clade of cactophilic Drosophila

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    Jackson Larry L

    2011-06-01

    Full Text Available Abstract Background We characterized variation and chemical composition of epicuticular hydrocarbons (CHCs in the seven species of the Drosophila buzzatii cluster with gas chromatography/mass spectrometry. Despite the critical role of CHCs in providing resistance to desiccation and involvement in communication, such as courtship behavior, mating, and aggregation, few studies have investigated how CHC profiles evolve within and between species in a phylogenetic context. We analyzed quantitative differences in CHC profiles in populations of the D. buzzatii species cluster in order to assess the concordance of CHC differentiation with species divergence. Results Thirty-six CHC components were scored in single fly extracts with carbon chain lengths ranging from C29 to C39, including methyl-branched alkanes, n-alkenes, and alkadienes. Multivariate analysis of variance revealed that CHC amounts were significantly different among all species and canonical discriminant function (CDF analysis resolved all species into distinct, non-overlapping groups. Significant intraspecific variation was found in different populations of D. serido suggesting that this taxon is comprised of at least two species. We summarized CHC variation using CDF analysis and mapped the first five CHC canonical variates (CVs onto an independently derived period (per gene + chromosome inversion + mtDNA COI gene for each sex. We found that the COI sequences were not phylogenetically informative due to introgression between some species, so only per + inversion data were used. Positive phylogenetic signal was observed mainly for CV1 when parsimony methods and the test for serial independence (TFSI were used. These results changed when no outgroup species were included in the analysis and phylogenetic signal was then observed for female CV3 and/or CV4 and male CV4 and CV5. Finally, removal of divergent populations of D. serido significantly increased the amount of phylogenetic signal as

  20. Drosophila egg chamber elongation

    OpenAIRE

    Gates, Julie

    2012-01-01

    As tissues and organs are formed, they acquire a specific shape that plays an integral role in their ability to function properly. A relatively simple system that has been used to examine how tissues and organs are shaped is the formation of an elongated Drosophila egg. While it has been known for some time that Drosophila egg elongation requires interactions between a polarized intracellular basal actin network and a polarized extracellular network of basal lamina proteins, how these interac...

  1. Dicty_cDB: VFN828 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 4841 ) Plasmodium falciparum 3D7 chromosome 11 section 6... 38 0.72 7 ( AY134811 ) Drosophila buzz...atii chromosome 2 clone 60.29.1-ch... 40 0.78 2 ( AY134809 ) Drosophila buzzatii chromosome

  2. Dicty_cDB: Contig-U11778-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available lum infernorum V4... 173 2e-41 AY219206_1( AY219206 |pid:none) Drosophila buzzatii isolate Ber-36... 169 4e-...40 CP000539_2973( CP000539 |pid:none) Acidovorax sp. JS42, complete g... 168 5e-40 AY219221_1( AY219221 |pid:none) Drosophila buzz...atii isolate Til-3 ... 167 8e-40 AY219209_1( AY219209 |pid:none) Drosophila buzz...atii isolate Cat-8 ... 167 8e-40 AY219220_1( AY219220 |pid:none) Drosophila buzzatii is...219218_1( AY219218 |pid:none) Drosophila buzzatii isolate Ceb-13... 167 1e-39 CP000358_394( CP000358 |pid:no

  3. Australian Aboriginal Astronomy: Overview

    CERN Document Server

    Norris, Ray P

    2013-01-01

    The traditional cultures of Aboriginal Australians include a significant astronomical component, perpetuated through oral tradition, ceremony, and art. This astronomical component includes a deep understanding of the motion of objects in the sky, and this knowledge was used for practical purposes, such as constructing calendars. There is also evidence that traditional Aboriginal Australians made careful records and measurements of cyclical phenomena, paid careful attention to unexpected phenomena such as eclipses and meteorite impacts, and could determine the cardinal points to an accuracy of a few degrees.

  4. Australian Curriculum Linked Lessons

    Science.gov (United States)

    Hurrell, Derek

    2013-01-01

    In providing a continued focus on tasks and activities that help to illustrate key ideas embedded in the new Australian Curriculum, the focus in this issue is on Measurement in the Measurement and Geometry strand. The small unit of work on measurement presented in this article has activities that can be modified to meet the requirements of…

  5. The Australian National University

    Institute of Scientific and Technical Information of China (English)

    李琳

    2007-01-01

    The Australian National University was established by Federal Parliament in 1946 with a mission to bring credit to the nation and to be one of the world’s great universities.It was the country’s only full-time research university at the time,and had no undergraduate teaching responsibilities.

  6. Australian Hackers and Ethics

    OpenAIRE

    Warren, M. J.; W. Hutchinson

    2003-01-01

    The aim of the paper is to look at the way hackers act and ways in which society can protect itself. The paper will show the current views and attitudes of hackers in an Australian context. The paper will also include a case study to show how a hacking incident can develop and how technology can be used to protect against hacking.

  7. Cancer in Drosophila

    DEFF Research Database (Denmark)

    Herranz, Héctor; Eichenlaub, Teresa; Cohen, Stephen M

    2016-01-01

    Cancer genomics has greatly increased our understanding of the complexity of the genetic and epigenetic changes found in human tumors. Understanding the functional relationships among these elements calls for the use of flexible genetic models. We discuss the use of Drosophila models to study...

  8. BMAA neurotoxicity in Drosophila.

    Science.gov (United States)

    Zhou, Xianchong; Escala, Wilfredo; Papapetropoulos, Spyridon; Bradley, Walter G; Zhai, R Grace

    2009-01-01

    We report the establishment of an in vivo model using the fruit fly Drosophila melanogaster to investigate the toxic effects of L-BMAA. We found that dietary intake of BMAA reduced the lifespan as well as the neurological functions of flies. Furthermore, we have developed an HPLC method to reliably detect both free and protein-bound BMAA in fly tissue extracts.

  9. Ecologically relevant stress resistance: from microarrays and quantitative trait loci to candidate genes – A research plan and preliminary results using Drosophila as a model organism and climatic and genetic stress as model stresses

    Indian Academy of Sciences (India)

    Volker Loeschcke; Jesper G Sørensen; Torsten N Kristensen

    2004-12-01

    We aim at studying adaptation to genetic and environmental stress and its evolutionary implications at different levels of biological organization. Stress influences cellular processes, individual physiology, genetic variation at the population level, and the process of natural selection. To investigate these highly connected levels of stress effects, it is advisable – if not critical – to integrate approaches from ecology, evolution, physiology, molecular biology and genetics. To investigate the mechanisms of stress resistance, how resistance evolves, and what factors contribute to and constrain its evolution, we use the well-defined model systems of Drosophila species, representing both cosmopolitan species such as D. melanogaster with a known genome map, and more specialized and ecologically well described species such as the cactophilic D. buzzatii. Various climate-related stresses are used as model stresses including desiccation, starvation, cold and heat. Genetic stress or genetic load is modelled by studying the consequences of inbreeding, the accumulation of (slightly) deleterious mutations, hybridization or the loss of genetic variability. We present here a research plan and preliminary results combining various approaches: molecular techniques such as microarrays, quantitative trait loci (QTL) analyses, quantitative PCR, ELISA or Western blotting are combined with population studies of resistance to climatic and genetic stress in natural populations collected across climatic gradients as well as in selection lines maintained in the laboratory.

  10. Australian Hackers and Ethics

    Directory of Open Access Journals (Sweden)

    M.J. Warren

    2003-05-01

    Full Text Available The aim of the paper is to look at the way hackers act and ways in which society can protect itself. The paper will show the current views and attitudes of hackers in an Australian context. The paper will also include a case study to show how a hacking incident can develop and how technology can be used to protect against hacking.

  11. Huntington disease in indigenous Australians.

    Science.gov (United States)

    Panegyres, P K; McGrath, F

    2008-02-01

    Huntington disease (HD) in indigenous Australians is a poorly analysed and difficult problem. This study addresses the issue of HD in remote indigenous Australian populations in the north-west of Western Australia. Proband identification, clinical assessment, neurogenetic studies and pedigree analysis led to the discovery of HD in the 63-year-old male proband and his family. HD in remote indigenous Australian communities is a challenging diagnostic and management problem compounded by the complexity of distance. PMID:18290828

  12. Drosophila by the dozen

    Energy Technology Data Exchange (ETDEWEB)

    Celniker, Susan E.; Hoskins, Roger A.

    2007-07-13

    This year's conference on Drosophila research illustratedwell the current focus of Drosophila genomics on the comprehensiveidentification of functional elements in the genome sequence, includingmRNA transcripts arising from multiple alternative start sites and splicesites, a multiplicity of noncoding transcripts and small RNAs,identification of binding sites for transcription factors, sequenceconservation in related species and sequence variation within species.Resources and technologies for genetics and functional genomics aresteadily being improved, including the building of collections oftransposon insertion mutants and hairpin constructs for RNA interference(RNAi). The conference also highlighted progress in the use of genomicinformation by many laboratories to study diverse aspects of biology andmodels of human disease. Here we will review a few highlights of especialinterest to readers of Genome Biology.

  13. Olfactory learning in Drosophila

    OpenAIRE

    Nehrkorn, Johannes

    2016-01-01

    Animals are able to form associative memories and benefit from past experience. In classical conditioning an animal is trained to associate an initially neutral stimulus by pairing it with a stimulus that triggers an innate response. The neutral stimulus is commonly referred to as conditioned stimulus (CS) and the reinforcing stimulus as unconditioned stimulus (US). The underlying neuronal mechanisms and structures are an intensely investigated topic. The fruit fly Drosophila melanogaster...

  14. Selective Autophagy in Drosophila

    Directory of Open Access Journals (Sweden)

    Ioannis P. Nezis

    2012-01-01

    Full Text Available Autophagy is an evolutionarily conserved process of cellular self-eating and is a major pathway for degradation of cytoplasmic material by the lysosomal machinery. Autophagy functions as a cellular response in nutrient starvation, but it is also associated with the removal of protein aggregates and damaged organelles and therefore plays an important role in the quality control of proteins and organelles. Although it was initially believed that autophagy occurs randomly in the cell, during the last years, there is growing evidence that sequestration and degradation of cytoplasmic material by autophagy can be selective. Given the important role of autophagy and selective autophagy in several disease-related processes such as neurodegeneration, infections, and tumorigenesis, it is important to understand the molecular mechanisms of selective autophagy, especially at the organismal level. Drosophila is an excellent genetically modifiable model organism exhibiting high conservation in the autophagic machinery. However, the regulation and mechanisms of selective autophagy in Drosophila have been largely unexplored. In this paper, I will present an overview of the current knowledge about selective autophagy in Drosophila.

  15. Initial neurogenesis in Drosophila.

    Science.gov (United States)

    Hartenstein, Volker; Wodarz, Andreas

    2013-01-01

    Early neurogenesis comprises the phase of nervous system development during which neural progenitor cells are born. In early development, the embryonic ectoderm is subdivided by a conserved signaling mechanism into two main domains, the epidermal ectoderm and the neurectoderm. Subsequently, cells of the neurectoderm are internalized and form a cell layer of proliferating neural progenitors. In vertebrates, the entire neurectoderm folds into the embryo to give rise to the neural tube. In Drosophila and many other invertebrates, a subset of neurectodermal cells, called neuroblasts (NBs), delaminates and forms the neural primordium inside the embryo where they divide in an asymmetric, stem cell-like mode. The remainder of the neurectodermal cells that stay behind at the surface loose their neurogenic potential and later give rise to the ventral part of the epidermis. The genetic and molecular analysis of the mechanisms controlling specification and proliferation of NBs in the Drosophila embryo, which played a significant part in pioneering the field of modern developmental neurobiology, represents the topic of this review. PMID:24014455

  16. Sterol requirements in Drosophila melanogaster

    OpenAIRE

    Almeida de Carvalho, Maria Joao

    2009-01-01

    Sterol is an abundant component of eukaryotic cell membranes and is thought to influence membrane properties such as permeability, fluidity and microdomain formation. Drosophila is an excellent model system in which to study functional requirements for membrane sterol because, although it does not synthesize sterol, it nevertheless requires sterols to complete development. Moreover, Drosophila normally incorporates sterols into cell membranes. Thus, dietary sterol depletion can be used to ...

  17. Chinese Rebalancing and Australian Exports

    OpenAIRE

    Gerard Kelly

    2014-01-01

    The Chinese authorities plan to gradually rebalance the composition of Chinese economic growth from investment towards household consumption. This article uses the World Input-Output Database (WIOD) to give a general sense of how this rebalancing might affect Australian exports and economic activity. Dollar for dollar, Chinese investment appears to absorb more than twice as much Australian value-added output as Chinese household consumption. This largely reflects the significant role of resou...

  18. An Australian Sense of Xenophobia

    OpenAIRE

    Linda Burney

    2009-01-01

    Linda Burney of the Wiradjuri Nation and Minister for Community Services in New South Wales discusses how xenophobia has manifested itself as forms of political and institutional racism in Australian history. She asks us to think of Australia as a giant and beautiful mosaic with over 200 Aboriginal Nations and for the rest of the Australian population to welcome ways to work with all its nation's people.

  19. Optogenetics in Drosophila Neuroscience.

    Science.gov (United States)

    Riemensperger, Thomas; Kittel, Robert J; Fiala, André

    2016-01-01

    Optogenetic techniques enable one to target specific neurons with light-sensitive proteins, e.g., ion channels, ion pumps, or enzymes, and to manipulate their physiological state through illumination. Such artificial interference with selected elements of complex neuronal circuits can help to determine causal relationships between neuronal activity and the effect on the functioning of neuronal circuits controlling animal behavior. The advantages of optogenetics can best be exploited in genetically tractable animals whose nervous systems are, on the one hand, small enough in terms of cell numbers and to a certain degree stereotypically organized, such that distinct and identifiable neurons can be targeted reproducibly. On the other hand, the neuronal circuitry and the behavioral repertoire should be complex enough to enable one to address interesting questions. The fruit fly Drosophila melanogaster is a favorable model organism in this regard. However, the application of optogenetic tools to depolarize or hyperpolarize neurons through light-induced ionic currents has been difficult in adult flies. Only recently, several variants of Channelrhodopsin-2 (ChR2) have been introduced that provide sufficient light sensitivity, expression, and stability to depolarize central brain neurons efficiently in adult Drosophila. Here, we focus on the version currently providing highest photostimulation efficiency, ChR2-XXL. We exemplify the use of this optogenetic tool by applying it to a widely used aversive olfactory learning paradigm. Optogenetic activation of a population of dopamine-releasing neurons mimics the reinforcing properties of a punitive electric shock typically used as an unconditioned stimulus. In temporal coincidence with an odor stimulus this artificially induced neuronal activity causes learning of the odor signal, thereby creating a light-induced memory.

  20. ASA24-Australian Version (Under Development)

    Science.gov (United States)

    In collaboration with the National Cancer Institute (NCI), a consortium of Australian Researchers is adapting the ASA24 system to the Australian context to account for variations in food consumed, portion sizes, and nutrient composition.

  1. Australian Cosmic Ray Modulation Research

    CERN Document Server

    Duldig, M L

    2000-01-01

    Australian research into variations of the cosmic ray flux arriving at the Earth has played a pivotal role for more than 50 years. The work has been largely led by the groups from the University of Tasmania and the Australian Antarctic Division and has involved the operation of neutron monitors and muon telescopes from many sites. In this paper the achievements of the Australian researchers are reviewed and future experiments are described. Particular highlights include: the determination of cosmic ray modulation parameters; the development of modelling techniques of Ground Level Enhancements; the confirmation of the Tail-In and Loss-Cone Sidereal anisotropies; the Space Ship Earth collaboration; and the Solar Cycle latitude survey.

  2. Changing Patterns of Governance for Australian Universities

    Science.gov (United States)

    Harman, Kay; Treadgold, Elaine

    2007-01-01

    Dissatisfaction with the "corporate" model for university governance, a model advocated by both sides of the Australian parliament and adopted by Australian universities over the past two decades, prompted the Australian Vice-Chancellors' Committee (AVCC) in 2003 to suggest an alternative "trusteeship" model. The paper discusses how this model…

  3. BOOMERANG - the Australian light source

    International Nuclear Information System (INIS)

    A proposal has been prepared for the installation in Australia of a national high performance synchrotron light facility called Boomerang. The Boomerang proposal had its origin in the establishment of the Australian Synchrotron Research Program (ASRP) which was one of the seven Major National Research Facilities announced by the Federal Government in December 1995. The ASRP provides the opportunity and funding for Australian researchers to access international synchrotron facilities, specifically two consortia at the Advanced Photon Source (APS) at the Argonne National Laboratory, USA and continued interaction with the Photon Factory at the KEK Laboratory in Japan. The ASRP was the successor to the Australian National Beamline Facility project (ANBF) which began in 1991 following the ASTEC inquiry titled 'Small Country - Big Science'. The Federal Government also provided funding for a Feasibility Study to determine the value of establishing an Australian-based synchrotron radiation facility. The Feasibility Study was completed in August 1998 and endorsed by the institutional members of the ASRP and the research community in general. The study concluded that, on the data available in Australia, there was a strong case for the installation of an Australian-based facility. The study considered several options for an Australian-based facility and recommended that these options and the data supporting the general conclusions receive further investigation. A mission was arranged to a select group of overseas laboratories to explore these questions in detail. The review team included a mix of scientific and industrial experience and also represented the interests of the ASRP and an Industrial Synchrotron Consortium based in Victoria. Based on the conclusions of the overseas mission and incorporating the advice of all international specialists in the design and use of synchrotron facilities consulted during the mission, the most cost-effective option was an extended

  4. Australian uranium and the election

    International Nuclear Information System (INIS)

    The international and national complexities of the situation in Australia over the question of mining of the country's large and rich uranium deposits are explored with especial reference to the pending general election. The present position is ironical since access to low cost uranium would give a welcome boost to the nuclear industry which is enthusiastically supported by the Australian prime minister and his colleagues yet the Australian government is unable to promote mining as rapidly as it would like because of the international commitments it has made to provide a justification for its policy. (U.K.)

  5. The Australian solar scene

    Energy Technology Data Exchange (ETDEWEB)

    Cowley, Paul [IT Power Australia (Australia)

    2007-06-15

    This presentation mainly talks about the actions taken by the Australian country concerning the use of renewable energy and the reduction of the peak load in some areas. In the first part, there are found both the geographical aspects as well as the major political, e.g. Asia-Pacific Partnership on Clean development and Climate. There are also explained the issues related to peak load growth and it is shown a comparison graphic having information about the most used photovoltaic systems. Then, there are mentioned the communities that are testing one of the model photovoltaic systems in order to: reduce the peak load, use the energy in a properly way, reduce the energy cost, among others. Finally, it is succinctly explained the photovoltaic rebate program as well as the use of the off-grid systems, besides, it is given relevant information about those remote communities of Australia and the benefits of the implementation of Bushlight. [Spanish] Esta presentacion trata primordialmente de las acciones, referentes al uso de energia renovable, tomadas por Australia y creadas con el fin de reducir la maxima demanda en algunas regiones de este pais. En la primera parte, se encuentran tanto los aspectos geograficos como los principales aspectos politicos; por ejemplo, la Sociedad Asia-Pacifico para el Desarrollo no Contaminante y el Clima. Asimismo, se da una explicacion acerca de las cuestiones relacionadas al crecimiento de la maxima demanda; ademas, se muestra un cuadro comparativo, que contiene informacion relacionada con los sistemas fotovoltaicos mas utilizados. Despues, se mencionan aquellas comunidades que tienen en periodo de prueba alguno de los modelos fotovoltaicos con el fin de: reducir la maxima demanda, utilizar eficientemente la energia, reducir el costo de la misma, entre otros aspectos mas. Finalmente, se explica escuetamente el programa de reembolso centrado en el uso de sistemas fotovoltaicos, asi como el uso de sistemas asilados de la red; ademas, se

  6. Staff Bullying in Australian Schools

    Science.gov (United States)

    Riley, Dan; Duncan, Deirdre J.; Edwards, John

    2011-01-01

    Purpose: The purpose of this paper is to estimate the prevalence of staff bullying in Australian schools, to identify bullies and targets and to examine some implications for school leaders in dealing with staff bullying. Design/methodology/approach: The quantitative research design survey instrument contained 11 demographic items, 44 questions of…

  7. Austrade Commissioner Tells Australian Market

    Institute of Scientific and Technical Information of China (English)

    Sheng; Jingjing; Sun; Yongjian

    2005-01-01

      As a large country with 7.69 million sq.km, is Australia a vast market for Chinese products such as cars and some traditional arts and crafts as people expect? With such questions bear in mind, China's Foreign Trade interviewed Mrs.Liu Bing, Commissioner of The Australian Trade Commission (Austrade). Let's hear what she said.……

  8. Austrade Commissioner Tells Australian Market

    Institute of Scientific and Technical Information of China (English)

    Sheng Jingjing; Sun Yongjian

    2005-01-01

    @@ As a large country with 7.69 million sq.km, is Australia a vast market for Chinese products such as cars and some traditional arts and crafts as people expect? With such questions bear in mind, China's Foreign Trade interviewed Mrs.Liu Bing, Commissioner of The Australian Trade Commission (Austrade). Let's hear what she said.

  9. Stress Literacy in Australian Adolescents

    Science.gov (United States)

    Varlow, Megan; Wuthrich, Viviana; Murrihy, Rachael; Remond, Louise; Tuqiri, Rebekka; van Kessel, Jacobine; Wheatley, Anna; Dedousis-Wallace, Anna; Kidman, Antony

    2009-01-01

    Stress literacy is a term that refers to knowledge about stress and stress management techniques. Levels of stress literacy were examined in more than nine hundred Australian adolescents by providing a short stress-management education session and assessing stress literacy using a pre-post survey design. It was found that while adolescents had a…

  10. Iron Absorption in Drosophila melanogaster

    Directory of Open Access Journals (Sweden)

    Fanis Missirlis

    2013-05-01

    Full Text Available The way in which Drosophila melanogaster acquires iron from the diet remains poorly understood despite iron absorption being of vital significance for larval growth. To describe the process of organismal iron absorption, consideration needs to be given to cellular iron import, storage, export and how intestinal epithelial cells sense and respond to iron availability. Here we review studies on the Divalent Metal Transporter-1 homolog Malvolio (iron import, the recent discovery that Multicopper Oxidase-1 has ferroxidase activity (iron export and the role of ferritin in the process of iron acquisition (iron storage. We also describe what is known about iron regulation in insect cells. We then draw upon knowledge from mammalian iron homeostasis to identify candidate genes in flies. Questions arise from the lack of conservation in Drosophila for key mammalian players, such as ferroportin, hepcidin and all the components of the hemochromatosis-related pathway. Drosophila and other insects also lack erythropoiesis. Thus, systemic iron regulation is likely to be conveyed by different signaling pathways and tissue requirements. The significance of regulating intestinal iron uptake is inferred from reports linking Drosophila developmental, immune, heat-shock and behavioral responses to iron sequestration.

  11. Iron Absorption in Drosophila melanogaster

    Science.gov (United States)

    Mandilaras, Konstantinos; Pathmanathan, Tharse; Missirlis, Fanis

    2013-01-01

    The way in which Drosophila melanogaster acquires iron from the diet remains poorly understood despite iron absorption being of vital significance for larval growth. To describe the process of organismal iron absorption, consideration needs to be given to cellular iron import, storage, export and how intestinal epithelial cells sense and respond to iron availability. Here we review studies on the Divalent Metal Transporter-1 homolog Malvolio (iron import), the recent discovery that Multicopper Oxidase-1 has ferroxidase activity (iron export) and the role of ferritin in the process of iron acquisition (iron storage). We also describe what is known about iron regulation in insect cells. We then draw upon knowledge from mammalian iron homeostasis to identify candidate genes in flies. Questions arise from the lack of conservation in Drosophila for key mammalian players, such as ferroportin, hepcidin and all the components of the hemochromatosis-related pathway. Drosophila and other insects also lack erythropoiesis. Thus, systemic iron regulation is likely to be conveyed by different signaling pathways and tissue requirements. The significance of regulating intestinal iron uptake is inferred from reports linking Drosophila developmental, immune, heat-shock and behavioral responses to iron sequestration. PMID:23686013

  12. Drosophila neuroblasts retain the daughter centrosome

    OpenAIRE

    Januschke, Jens; Llamazares, Salud; Reina, Jose; Gonzalez, Cayetano

    2011-01-01

    During asymmetric mitosis, both in male Drosophila germline stem cells and in mouse embryo neural progenitors, the mother centrosome is retained by the self-renewed cell; hence suggesting that mother centrosome inheritance might contribute to stemness. We test this hypothesis in Drosophila neuroblasts (NBs) tracing photo converted centrioles and a daughter-centriole-specific marker generated by cloning the Drosophila homologue of human Centrobin. Here we show that upon asymmetric mitosis, the...

  13. Signal Propagation in Drosophila Central Neurons

    OpenAIRE

    Gouwens, Nathan W.; Wilson, Rachel I.

    2009-01-01

    Drosophila is an important model organism for investigating neural development, neural morphology, neurophysiology, and neural correlates of behaviors. However, almost nothing is known about how electrical signals propagate in Drosophila neurons. Here we address these issues in antennal lobe projection neurons (PNs), one of the most well-studied classes of Drosophila neurons. We use morphological and electrophysiological data to deduce the passive membrane properties of these neurons and to b...

  14. Nazi medical experiments on Australian prisoners of war: Commentary on the testimony of an Australian soldier.

    Science.gov (United States)

    Weisz, George M

    2015-12-01

    Archival research reveals that Australian prisoners of war were exposed to non-consensual medical experiments during World War II. This article discusses the first known case of an Australian soldier exposed to German medical experiments. PMID:26939510

  15. Nazi medical experiments on Australian prisoners of war: Commentary on the testimony of an Australian soldier.

    Science.gov (United States)

    Weisz, George M

    2015-12-01

    Archival research reveals that Australian prisoners of war were exposed to non-consensual medical experiments during World War II. This article discusses the first known case of an Australian soldier exposed to German medical experiments.

  16. Australian network of magnetic observatories

    Science.gov (United States)

    Barton, C. E.

    Six magnetic observatories are presently operated by the Australian Bureau of Mineral Resources, Geology and Geophysics (BMR), with assistance from various other organizations. Variometer recordings are made of three or more elements of the field at minute intervals, and absolute measurements are made weekly. There are four observatories on the continent (Canberra, Gnangara, Charters Towers, and Learmonth), one on Macquarie Island, and one at Mawson Station in eastern Antarctica (Figure 1). In addition, semiweekly absolute observations of the field (D, H, and F) are made at the other two permanent Australian Antarctic bases (Casey and Davis). A three-axis fluxgate magnetometer (EDA Electronics, Toronto , Canada) is operated independently by the Upper Atmosphere Physics group at Davis. Monthly mean values, K indices, and information about magnetic disturbances are published monthly in the BMR Geophysical Observatory Report.

  17. Research Output of Australian Universities

    OpenAIRE

    Malcolm Abbot; Hristos Doucouliagos

    2003-01-01

    Research plays an important role in underpinning a country’s economic and social life. Universities are at the centre of the research and human capital generating process. The aim of this paper is to explore the links between research output, research income, academic and non-academic labour and some of the characteristics of Australian universities. The results indicate that research income, academic staff and post-graduates are all positively associated with research output. There are notic...

  18. Meteors in Australian Aboriginal Dreamings

    Science.gov (United States)

    Hamacher, Duane W.; Norris, Ray P.

    2010-06-01

    We present a comprehensive analysis of Australian Aboriginal accounts of meteors. The data used were taken from anthropological and ethnographic literature describing oral traditions, ceremonies, and Dreamings of 97 Aboriginal groups representing all states of modern Australia. This revealed common themes in the way meteors were viewed between Aboriginal groups, focusing on supernatural events, death, omens, and war. The presence of such themes around Australia was probably due to the unpredictable nature of meteors in an otherwise well-ordered cosmos.

  19. Time Travel: Australian Tourists and Britain's Past

    OpenAIRE

    Richard White

    2012-01-01

    Across the twentieth century, Britain drew more Australian tourists for longer and more intense experiences than anywhere else, though as early as the 1970s Asia was attracting more Australians than Europe. They found much to admire and to deprecate in Britain but above all they were seduced by Britain’s past, or what they imagined it to be. This paper examines the Australian experience of history in Britain, their admiration for notions of tradition, for an unchanging village life, for fadin...

  20. Integrative Model of Drosophila Flight

    OpenAIRE

    Dickson, William B.; Andrew D Straw; Dickinson, Michael H

    2008-01-01

    This paper presents a framework for simulating the flight dynamics and control strategies of the fruit fly Drosophila melanogaster. The framework consists of five main components: an articulated rigid-body simulation, a model of the aerodynamic forces and moments, a sensory systems model, a control model, and an environment model. In the rigid-body simulation the fly is represented by a system of three rigid bodies connected by a pair of actuated ball joints. At each instant of th...

  1. Chromatin assembly using Drosophila systems.

    Science.gov (United States)

    Fyodorov, Dmitry V; Levenstein, Mark E

    2002-05-01

    To successfully study chromatin structure and activity in vitro, it is essential to have a chromatin assembly system that will prepare extended nucleosome arrays with highly defined protein content that resemble bulk chromatin isolated from living cell nuclei in terms of periodicity and nucleosome positioning. The Drosophila ATP-dependent chromatin assembly system described in this unit meets these requirements. The end product of the reaction described here has highly periodic extended arrays with physiologic spacing and positioning of the nucleosomes.

  2. Visual attention in Drosophila melanogaster

    OpenAIRE

    Sareen, Preeti

    2012-01-01

    There is such vast amount of visual information in our surroundings at any time that filtering out the important information for further processing is a basic requirement for any visual system. This is accomplished by deploying attention to focus on one source of sensory inputs to the exclusion of others (Luck and Mangun 2009). Attention has been studied extensively in humans and non human primates (NHPs). In Drosophila, visual attention was first demonstrated in 1980 (Wolf and Heisenberg 198...

  3. Insulin receptor in Drosophila melanogaster

    Energy Technology Data Exchange (ETDEWEB)

    Petruzzelli, L.; Herrera, R.; Rosen, O.

    1986-05-01

    A specific, high affinity insulin receptor is present in both adult Drosophila and in Drosophila embryos. Wheat germ lectin-enriched extracts of detergent-solubilized membranes from embryos and adults bind insulin with a K/sub d/ of 15 nM. Binding is specific for insulin; micromolar concentrations of proinsulin, IGFI, and IGFII are required to displace bound /sup 125/I-insulin. Insulin-dependent protein tyrosine kinase activity appears during embryogenesis. It is evident between 6 and 12 hours of development, peaks between 12 and 18 hours and falls in the adult. During 0-6 hours of embryogenesis, and in the adult, a specific protein band (Mr = 135,000) is crosslinked to /sup 125/I-insulin. During 6-12 and 12-18 hours of embryogenesis stages in which insulin-dependent protein tyrosine kinase is high, an additional band (Mr = 100,000) becomes crosslinked to /sup 125/I-insulin. Isolation and DNA sequence analysis of genomic clones encoding the Drosophila insulin receptor will be presented as will the characterization of insulin receptor mRNA's during development.

  4. Insulin receptor in Drosophila melanogaster

    International Nuclear Information System (INIS)

    A specific, high affinity insulin receptor is present in both adult Drosophila and in Drosophila embryos. Wheat germ lectin-enriched extracts of detergent-solubilized membranes from embryos and adults bind insulin with a K/sub d/ of 15 nM. Binding is specific for insulin; micromolar concentrations of proinsulin, IGFI, and IGFII are required to displace bound 125I-insulin. Insulin-dependent protein tyrosine kinase activity appears during embryogenesis. It is evident between 6 and 12 hours of development, peaks between 12 and 18 hours and falls in the adult. During 0-6 hours of embryogenesis, and in the adult, a specific protein band (Mr = 135,000) is crosslinked to 125I-insulin. During 6-12 and 12-18 hours of embryogenesis stages in which insulin-dependent protein tyrosine kinase is high, an additional band (Mr = 100,000) becomes crosslinked to 125I-insulin. Isolation and DNA sequence analysis of genomic clones encoding the Drosophila insulin receptor will be presented as will the characterization of insulin receptor mRNA's during development

  5. 'Peer pressure' in larval Drosophila?

    Science.gov (United States)

    Niewalda, Thomas; Jeske, Ines; Michels, Birgit; Gerber, Bertram

    2014-01-01

    Understanding social behaviour requires a study case that is simple enough to be tractable, yet complex enough to remain interesting. Do larval Drosophila meet these requirements? In a broad sense, this question can refer to effects of the mere presence of other larvae on the behaviour of a target individual. Here we focused in a more strict sense on 'peer pressure', that is on the question of whether the behaviour of a target individual larva is affected by what a surrounding group of larvae is doing. We found that innate olfactory preference of a target individual was neither affected (i) by the level of innate olfactory preference in the surrounding group nor (ii) by the expression of learned olfactory preference in the group. Likewise, learned olfactory preference of a target individual was neither affected (iii) by the level of innate olfactory preference of the surrounding group nor (iv) by the learned olfactory preference the group was expressing. We conclude that larval Drosophila thus do not take note of specifically what surrounding larvae are doing. This implies that in a strict sense, and to the extent tested, there is no social interaction between larvae. These results validate widely used en mass approaches to the behaviour of larval Drosophila. PMID:24907371

  6. Using Drosophila for Studies of Intermediate Filaments.

    Science.gov (United States)

    Bohnekamp, Jens; Cryderman, Diane E; Thiemann, Dylan A; Magin, Thomas M; Wallrath, Lori L

    2016-01-01

    Drosophila melanogaster is a useful organism for determining protein function and modeling human disease. Drosophila offers a rapid generation time and an abundance of genomic resources and genetic tools. Conservation in protein structure, signaling pathways, and developmental processes make studies performed in Drosophila relevant to other species, including humans. Drosophila models have been generated for neurodegenerative diseases, muscular dystrophy, cancer, and many other disorders. Recently, intermediate filament protein diseases have been modeled in Drosophila. These models have revealed novel mechanisms of pathology, illuminated potential new routes of therapy, and make whole organism compound screens feasible. The goal of this chapter is to outline steps to study intermediate filament function and model intermediate filament-associated diseases in Drosophila. The steps are general and can be applied to study the function of almost any protein. The protocols outlined here are for both the novice and experienced Drosophila researcher, allowing the rich developmental and cell biology that Drosophila offers to be applied to studies of intermediate filaments.

  7. Radioresistance and radiosensitivity in Drosophila melanogaster

    International Nuclear Information System (INIS)

    Studying the mechanisms controlling radioresistant in Drosophila the sensibility of four strains of Drosophila melanogaster to sex-linked recessive lethal mutations induced by 5kR Cobalt-60 gamma radiation and 0,006 M EMS or 0,25% of caffeine was determined. (M.A.C.)

  8. Australian internet histories: Past, present and future

    DEFF Research Database (Denmark)

    Brügger, Niels

    2012-01-01

    This Afterword compares the articles in this issue of Media International Australia to the ‘first wave’ of Australian internet historiography, a field of study established by Australian internet scholars around 2000. After identifying what is new in the present issue, I outline four paths that may...

  9. The sociology of the Australian agricultural environment.

    NARCIS (Netherlands)

    Vanclay, F.

    1994-01-01

    Australian agriculture is in crisis, the terms of trade for agriculture are falling, many farmers have negative incomes, and there is massive structural adjustment with government policy assisting the exit of marginal farmers out of agriculture. Australian governments are gripped with the philosophy

  10. A global history of Australian trees.

    Science.gov (United States)

    Bennett, Brett M

    2011-01-01

    Scholars studying the globalization of Australian trees have previously emphasized the rapid natural propagation of Australian trees outside of their native habitats, believing their success to be a reversal of "ecological imperialism" from the "new world" to the "old world." This article argues that the expansion of Australian trees should not be viewed as a biological phenomenon, but as the result of a long-term attempt by powerful states and state-sponsored scientists to select and breed Australian species that could grow in a variety of climates and ecological conditions. Five non-biological factors largely determined the success of these attempts to grow Australian trees: the abundance or paucity of natural forests, state power, the amount of scientific research directed to planting Australian trees, the cost of labor, and the ability to utilize hardwood timbers and bark. This paper compares the use of Australian trees in Australia, India, and South Africa to demonstrate that biology was not the determining factor in the long-term success of many Australian genera and species. PMID:20665086

  11. Four Management Agendas for Australian Universities

    Science.gov (United States)

    Sharrock, Geoff

    2012-01-01

    In a new mixed economy of higher learning, Australian universities require more strategic management to compete and collaborate sustainably. However, many scholars argue that new modes of university management are at odds with scholarly aims and values. This article examines how Australian universities frame their missions and communicate their…

  12. Recent developments in the Australian housing market

    OpenAIRE

    James Bond

    2003-01-01

    Housing plays an important role in Australia’s economic growth and in the welfare of Australians. This article examines developments in the Australian housing market over recent years. It argues that investors have played an increasingly important role in the housing market for both detached houses and apartments.

  13. Succession Planning in Australian Farming

    Directory of Open Access Journals (Sweden)

    John Hicks

    2012-11-01

    Full Text Available The theme of this paper is that succession planning in Australian farming is under-developed.It may be linked to economic and social change which suggests that farmers need to adapt togenerational change but this is being resisted or ignored. The implications of this are the slowdecline of family farming, a poor transfer of skills and knowledge to subsequent generationsof farmers in some parts of the agricultural sector and the potential for an extension of thefinancial services industry to develop a more effective raft of succession planning measuresto mitigate the effects of a traditional approach to succession in agriculture.

  14. Australian developments in marine science

    Science.gov (United States)

    Coffin, Millard F.

    2012-07-01

    Australia is an island nation with about two thirds of its jurisdiction underwater. On 25 May 2012, Australia instituted the Seas and Submerged Lands (Limits of Continental Shelf) Proclamation 2012, confirming areas of seabed where Australia has exclusive rights to explore and exploit marine resources. This proclamation follows recommendations by the Commission on the Limits of the Continental Shelf, a body established under the United Nations Convention on the Law of the Sea, confirming Australia's entitlement to extended continental shelf, i.e., that beyond 200 nautical miles from the coastline, of some 2.56 million square kilometers, excluding Australian Antarctic Territory [Symonds et al., 2009] (Figure 1a).

  15. Australian Aboriginal Astronomy and Cosmology

    Science.gov (United States)

    Clarke, Philip A.

    Australian Aboriginal ethnoastronomical traditions were recorded from a wide variety of sources in different periods. While the corpus of mythology concerning the heavens is diverse, it is unified by beliefs of a Skyworld as land with its own topography, containing plants and animals familiar to those living below. Spirits of the dead reside alongside the Creation Ancestors as celestial bodies in the Skyworld. Aboriginal hunter-gatherers used the regular movement of constellations and planets to measure time and to indicate the season, while unexpected change in the sky was seen as an omen.

  16. Comets in Australian Aboriginal Astronomy

    CERN Document Server

    Hamacher, Duane W

    2010-01-01

    We present 25 accounts of comets from 40 Australian Aboriginal communities, citing both supernatural perceptions of comets and historical accounts of bright comets. Historical and ethnographic descriptions include the Great Comets of 1843, 1861, 1901, 1910, and 1927. We describe the perceptions of comets in Aboriginal societies and show that they are typically associated with fear, death, omens, malevolent spirits, and evil magic, consistent with many cultures around the world. We also provide a list of words for comets in 16 different Aboriginal languages.

  17. Comets in Australian Aboriginal Astronomy

    Science.gov (United States)

    Hamacher, Duane W.; Norris, Ray P.

    2011-03-01

    We present 25 accounts of comets from 40 Australian Aboriginal communities, citing both supernatural perceptions of comets and historical accounts of historically bright comets. Historical and ethnographic descriptions include the Great Comets of 1843, 1861, 1901, 1910, and 1927. We describe the perceptions of comets in Aboriginal societies and show that they are typically associated with fear, death, omens, malevolent spirits, and evil magic, consistent with many cultures around the world. We also provide a list of words for comets in 16 different Aboriginal languages.

  18. Aurorae in Australian Aboriginal Traditions

    CERN Document Server

    Hamacher, Duane W

    2013-01-01

    Transient celestial phenomena feature prominently in the astronomical knowledge and traditions of Aboriginal Australians. In this paper, I collect accounts of the Aurora Australis from the literature regarding Aboriginal culture. Using previous studies of meteors, eclipses, and comets in Aboriginal traditions, I anticipate that the physical properties of aurora, such as their generally red colour as seen from southern Australia, will be associated with fire, death, blood, and evil spirits. The survey reveals this to be the case and also explores historical auroral events in Aboriginal cultures, aurorae in rock art, and briefly compares Aboriginal auroral traditions with other global indigenous groups, including the Maori of New Zealand.

  19. Aurorae in Australian Aboriginal Traditions

    Science.gov (United States)

    Hamacher, Duane W.

    2013-07-01

    Transient celestial phenomena feature prominently in the astronomical knowledge and traditions of Aboriginal Australians. In this paper, I collect accounts of the Aurora Australis from the literature regarding Aboriginal culture. Using previous studies of meteors, eclipses, and comets in Aboriginal traditions, I anticipate that the physical properties of aurora, such as their generally red colour as seen from southern Australia, will be associated with fire, death, blood, and evil spirits. The survey reveals this to be the case and also explores historical auroral events in Aboriginal cultures, aurorae in rock art, and briefly compares Aboriginal auroral traditions with other global indigenous groups, including the Maori of New Zealand.

  20. Learner discipline: An Australian perspective

    Directory of Open Access Journals (Sweden)

    D. Stewart

    2004-07-01

    Full Text Available Australian schools by and large are safe schools. Nonetheless discipline problems do exist – including bullying behaviour. For this kind of problem schools should have management policies in place. As traditional behaviour-management practices – including corporal punishment – are largely prohibited in Australian schools, contemporary practices centre on management through supportive school programmes, including appropriate curricula and school-support structures. This article supports the belief that measures such as the exclusion of misbehaving learners should be treated with caution. Measures such as this might not reflect accepted international principles and practices and should only be exercised in the most extreme circumstances. The article also supports the view that it is part of the school’s role to ensure that all learners are aware of the reality that while they have rights, they also have corresponding responsibilities. This awareness is more likely to be achieved in a supportive school culture where each learner is recognised as having unique qualities that can mature and grow in an appropriate learning environment.

  1. Taste processing in Drosophila larvae

    Directory of Open Access Journals (Sweden)

    Anthi A. Apostolopoulou

    2015-10-01

    Full Text Available The sense of taste allows animals to detect chemical substances in their environment to initiate appropriate behaviors: to find food or a mate, to avoid hostile environments and predators. Drosophila larvae are a promising model organism to study gustation. Their simple nervous system triggers stereotypic behavioral responses, and the coding of taste can be studied by genetic tools at the single cell level. This review briefly summarizes recent progress on how taste information is sensed and processed by larval cephalic and pharyngeal sense organs. The focus lies on several studies, which revealed cellular and molecular mechanisms required to process sugar, salt, and bitter substances.

  2. Complete mitochondrial genome of Drosophila albomicans.

    Science.gov (United States)

    Kang, Xiongbin; Luo, Xiao; Zhang, Zhi; Zhang, Zhen; Yang, Junqing; Bi, Guiqi

    2016-09-01

    Drosophila albomicans has been widely used as an important animal model for chromosome evolution. In this study, the mitochondrial genome sequence of this species is determined and described for the first time. The mitochondrial genome (15 849 bp) encompasses two rRNA, 22 tRNA, and 13 protein-coding genes. Genome content and structure are similar to those reported from other Drosophila mitochondrial genomes. Phylogeny analysis indicates that D. albomicans have a closer genetic relationship with Drosophil aincompta and Drosophil alittoralis. This mitochondrial genome is potentially important for studying molecular evolution and conservation genetics in Drosophila genus. PMID:26358579

  3. The Portrayal of Indigenous Health in Selected Australian Media

    OpenAIRE

    Melissa J. Stoneham; Jodie Goodman; Mike Daube

    2014-01-01

    It is acknowledged that health outcomes for Australian Indigenous peoples are lower than those of non-Indigenous Australians. Research suggests negative media in relation to Indigenous Australians perpetuates racist stereotypes among the wider population and impacts on the health of Indigenous Australians. This study examined the media portrayal of Indigenous Australian public health issues in selected media over a twelve month period and found that, overwhelmingly, the articles were negative...

  4. Compliance with Corporate Governance Principles: Australian Evidence

    Directory of Open Access Journals (Sweden)

    Maryam Safari

    2015-12-01

    Full Text Available This study investigates the association between the level of compliance of Australian listed companies with Australian corporate governance principles, in aggregate, and the level of discretionary accruals using the modified Jones model. It is hypothesised that higher levels of compliance would be associated with lower levels of discretionary accruals. Data from a random sample of 214 Australian listed companies for the years 2009 and 2010 were used to test the hypothesis. The results demonstrate a significant negative relationship indicating that companies with higher levels of compliance engage in lower levels of earnings management via discretionary accruals.

  5. A new opportunity for Australian uranium

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-07-01

    This study analyses the outlook for the world uranium industry and includes projections of uranium demand, supply and prices over the next decade and a comparison with other forecasts. The potential increases in Australian output are quantified, under both continuation of the three mine policy and an open mine policy, as well as the potential impact on the world uranium market, using the well known ORANI model of the Australian economy. It is estimated that Australian output could almost double by 2004 if the three mine policy were abolished. 53 refs., 20 tabs., 6 figs.

  6. A new opportunity for Australian uranium

    International Nuclear Information System (INIS)

    This study analyses the outlook for the world uranium industry and includes projections of uranium demand, supply and prices over the next decade and a comparison with other forecasts. The potential increases in Australian output are quantified, under both continuation of the three mine policy and an open mine policy, as well as the potential impact on the world uranium market, using the well known ORANI model of the Australian economy. It is estimated that Australian output could almost double by 2004 if the three mine policy were abolished. 53 refs., 20 tabs., 6 figs

  7. Australian national residue survey – closing the loop on pesticide residue risk management for Australian grain

    OpenAIRE

    Reichstein, I.; Healy, K; James, A.; Murray, B.

    2010-01-01

    Australia exports a major proportion of its agricultural production and is highly dependent on maintaining and developing access to, and competitiveness in, export markets. To preserve Australia’s status as a provider of high quality grain, the majority of Australian primary producers rely on pesticides to protect their crops from pests and diseases, particularly in post-harvest situations. The Australian Pesticides and Veterinary Medicines Authority (APVMA) supports Australian agriculture by...

  8. Eclipses in Australian Aboriginal Astronomy

    CERN Document Server

    Hamacher, Duane W

    2011-01-01

    We explore 50 Australian Aboriginal accounts of lunar and solar eclipses to determine how Aboriginal groups understood this phenomenon. We summarise the literature on Aboriginal references to eclipses, showing that many Aboriginal groups viewed eclipses negatively, frequently associating them with bad omens, evil magic, disease, blood and death. In many communities, Elders or medicine men were believed to have the ability to control or avert eclipses by magical means, solidifying their role as provider and protector within the community. We also show that many Aboriginal groups understood the motions of the sun-earth-moon system, the connection between the lunar phases and tides, and acknowledged that solar eclipses were caused by the moon blocking the sun.

  9. Australians' attitudes to nuclear disarmament

    International Nuclear Information System (INIS)

    The results of a series of surveys of 2900 Australians show that some 80 per cent favour nuclear disarmament. The nuclear disarmament view is broad-based, for example it cuts across differences in age, sex and education. However the view is more common among people towards the left of the political spectrum who view the world as benign rather than hostile and who consider stockpiles can be reduced by small, reciprocated and supervised reductions. Between 2.5 per cent and 5.5 per cent of respondents act to bring about nuclear disarmament. The findings support and extend results from studies outside Australia showing that attitudes favouring nuclear disarmament are distributing themselves widely

  10. A Drosophila metallophosphoesterase mediates deglycosylation of rhodopsin

    OpenAIRE

    Cao, Jinguo; Li, Yi; Xia, Wenjing; Reddig, Keith; Hu, Wen; XIE, Wei; Li, Hong-Sheng; Han, Junhai

    2011-01-01

    The glycosylation status of Rhodopsin controls its trafficking and stability, and is hence critical for photoreceptor function. Here, a Drosophila metallophosphoesterase is identified that affects Rhodopsin glycosylation by regulating the activity of an enzyme involved in glycan processing.

  11. Modeling tumor invasion and metastasis in Drosophila

    Directory of Open Access Journals (Sweden)

    Wayne O. Miles

    2011-11-01

    Full Text Available Conservation of major signaling pathways between humans and flies has made Drosophila a useful model organism for cancer research. Our understanding of the mechanisms regulating cell growth, differentiation and development has been considerably advanced by studies in Drosophila. Several recent high profile studies have examined the processes constraining the metastatic growth of tumor cells in fruit fly models. Cell invasion can be studied in the context of an in vivo setting in flies, enabling the genetic requirements of the microenvironment of tumor cells undergoing metastasis to be analyzed. This Perspective discusses the strengths and limitations of Drosophila models of cancer invasion and the unique tools that have enabled these studies. It also highlights several recent reports that together make a strong case for Drosophila as a system with the potential for both testing novel concepts in tumor progression and cell invasion, and for uncovering players in metastasis.

  12. Lipid metabolism in Drosophila: development and disease

    Institute of Scientific and Technical Information of China (English)

    Zhonghua Liu; Xun Huang

    2013-01-01

    Proteins,nucleic acids,and lipids are three major components of the cell.Despite a few basic metabolic pathways,we know very little about lipids,compared with the explosion of knowledge about proteins and nucleic acids.How many different forms of lipids are there? What are the in vivo functions of individual lipid? How does lipid metabolism contribute to normal development and human health? Many of these questions remain unanswered.For over a century,the fruit fly Drosophila melanogaster has been used as a model organism to study basic biological questions.In recent years,increasing evidences proved that Drosophila models are highly valuable for lipid metabolism and energy homeostasis researches.Some recent progresses of lipid metabolic regulation during Drosophila development and in Drosophila models of human diseases will be discussed in this review.

  13. Behavioral modification in choice process of Drosophila

    Institute of Scientific and Technical Information of China (English)

    WANG; Shunpeng; (王顺鹏); TANG; Shiming; (唐世明); LI; Yan; (李; 岩); GUO; Aike; (郭爱克)

    2003-01-01

    In visual operant conditioning of Drosophila at the flight simulator, only motor output of flies--yaw torque--is recorded, which is involved in the conditioning process. The current study used a newly-designed data analysis method to study the torque distribution of Drosophila. Modification of torque distribution represents the effects of operant conditioning on flies' behavioral mode. Earlier works[10] showed that, when facing contradictory visual cues, flies could make choices based upon the relative weightiness of different cues, and it was demonstrated that mushroom bodies might play an important role in such choice behavior. The new "torque-position map" method was used to explore the CS-US associative learning and choice behavior in Drosophila from the aspect of its behavioral mode. Finally, this work also discussed various possible neural bases involved in visual associative learning, choice processing and modification processing of the behavioral mode in the visual operant conditioning of Drosophila.

  14. Population and Australian development assistance.

    Science.gov (United States)

    Jones, R

    1992-07-01

    Australia's position on international population issues is consistent with the major international statements on population: the World Population Plan of Action (1974), the Mexico City Declaration (1984), and the Amsterdam Declaration (1989). Australia's policy emphasizes the importance of population policies as an integral part of social, economic, and cultural development aimed at improving the quality of life of the people. Factors that would promote smaller families include improving economic opportunities, old-age security, education and health (particularly for women), as well as improving the accessibility and quality of family planning services. The quality of care approach is directly complementary to the Australian International Development Assistance Bureau (AIDAB)'s Women-In-Development Policy and its Health Policy, which stresses the theme of Women And Their Children's Health (WATCH). Australia's support for population programs and activities has increased considerably over the last few years. Total assistance for the year 1990/91 was around $7 million out of a total aid program of $1216 million. In recent years AIDAB has funded family planning activities or health projects with family planning components in a number of countries in the Asia-Pacific region. In the South Pacific region AIDAB has funded a reproductive health video project taking into consideration the cultural sensitivities and customs of the peoples of the region. AIDAB has supported a UN Population Fund project in Thailand that aims to strengthen the capacity of the National Statistical Office to collect population data. The US currently accounts for around 40% of all population-related development assistance to improve the health of women and children through family planning. The other major donors are Japan, the Scandinavian countries, and the Netherlands. Funding for population has been a relatively low percentage of overall development assistance budgets in OECD countries. In the

  15. Genetic Determinants of Phosphate Response in Drosophila

    OpenAIRE

    Clemens Bergwitz; Wee, Mark J.; Sumi Sinha; Joanne Huang; Charles DeRobertis; Mensah, Lawrence B.; Jonathan Cohen; Adam Friedman; Meghana Kulkarni; Yanhui Hu; Arunachalam Vinayagam; Michael Schnall-Levin; Bonnie Berger; Perkins, Lizabeth A.; Mohr, Stephanie E.

    2012-01-01

    Phosphate is required for many important cellular processes and having too little phosphate or too much can cause disease and reduce life span in humans. However, the mechanisms underlying homeostatic control of extracellular phosphate levels and cellular effects of phosphate are poorly understood. Here, we establish Drosophila melanogaster as a model system for the study of phosphate effects. We found that Drosophila larval development depends on the availability of phosphate in the medium. ...

  16. The Drosophila cyst stem cell lineage

    OpenAIRE

    Zoller, Richard; Schulz, Cordula

    2012-01-01

    In all animals, germline cells differentiate in intimate contact with somatic cells and interactions between germline and soma are particularly important for germline development and function. In the male gonad of Drosophila melanogaster, the developing germline cells are enclosed by somatic cyst cells. The cyst cells are derived from cyst stem cells (CySCs) of somatic origin and codifferentiate with the germline cells. The fast generation cycle and the genetic tractability of Drosophila has ...

  17. RNA Binding Specificity of Drosophila Muscleblind†

    OpenAIRE

    Goers, Emily S.; Voelker, Rodger B.; Gates, Devika P.; Berglund, J. Andrew

    2008-01-01

    Members of the muscleblind family of RNA binding proteins found in Drosophila and mammals are key players in both the human disease myotonic dystrophy and the regulation of alternative splicing. Recently, the mammalian muscleblind-like protein, MBNL1, has been shown to have interesting RNA binding properties with both endogenous and disease-related RNA targets. Here we report the characterization of RNA binding properties of the Drosophila muscleblind protein Mbl. Mutagenesis of double-strand...

  18. The Digestive Tract of Drosophila melanogaster

    OpenAIRE

    Bassler, Bl; Lichten, M; Schupbach, G.; Lemaitre, Bruno; Miguel-Aliaga, Irene

    2013-01-01

    The digestive tract plays a central role in the digestion and absorption of nutrients. Far from being a passive tube, it provides the first line of defense against pathogens and maintains energy homeostasis by exchanging neuronal and endocrine signals with other organs. Historically neglected, the gut of the fruit fly Drosophila melanogaster has recently come to the forefront of Drosophila research. Areas as diverse as stem cell biology, neurobiology, metabolism, and immunity are benefitting ...

  19. Responding to Indigenous Australian Sexual Assault

    Directory of Open Access Journals (Sweden)

    Janya McCalman

    2014-01-01

    Full Text Available Indigenous Australians experience a high prevalence of sexual assault, yet a regional sexual assault service found few Indigenous Australians accessed their services. This prompted exploration of how its services might be improved. A resultant systematic search of the literature is reported in this article. Seven electronic databases and seven websites were systematically searched for peer reviewed and gray literature documenting responses to the sexual assault of Indigenous Australians. These publications were then classified by response type and study type. Twenty-three publications met the inclusion criteria. They included studies of legal justice, media, and community-based and mainstream service responses for Indigenous survivors and perpetrators. We located program descriptions, measurement, and descriptive research, but no intervention studies. There is currently insufficient evidence to confidently prescribe what works to effectively respond to Indigenous Australian sexual assault. The study revealed an urgent need for researchers, Indigenous communities, and services to work together to develop the evidence base.

  20. The Australian synchrotron; Le synchrotron australien

    Energy Technology Data Exchange (ETDEWEB)

    Farhi, R

    2005-06-15

    This document recalls the historical aspects of the Australian Synchrotron which will be implemented in 2007. It presents then the objectives of this program, the specifications of the ring and the light lines. (A.L.B.)

  1. Innovation in Australian Workplaces: An Empirical Analysis.

    Science.gov (United States)

    Rogers, Mark

    1999-01-01

    The determinants of innovation were examined using data from 698 Australian workplaces. Results suggest that better employee-management communications are associated with more change and that workplaces with higher levels of training undergo more change. (Author/JOW)

  2. China's first Australian Garden opens in Guangzhou

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ The opening for the Australian Garden was jointly held by the BHP Billiton China and the CAS South China Botanical Garden (SCBG) in Guangzhou, capital of south China's Guangdong Province on 18 January.

  3. A Drosophila Model for Screening Antiobesity Agents

    Science.gov (United States)

    Men, Tran Thanh; Thanh, Duong Ngoc Van; Yamaguchi, Masamitsu; Suzuki, Takayoshi; Hattori, Gen; Arii, Masayuki; Huy, Nguyen Tien; Kamei, Kaeko

    2016-01-01

    Although triacylglycerol, the major component for lipid storage, is essential for normal physiology, its excessive accumulation causes obesity in adipose tissue and is associated with organ dysfunction in nonadipose tissue. Here, we focused on the Drosophila model to develop therapeutics for preventing obesity. The brummer (bmm) gene in Drosophila melanogaster is known to be homologous with human adipocyte triglyceride lipase, which is related to the regulation of lipid storage. We established a Drosophila model for monitoring bmm expression by introducing the green fluorescent protein (GFP) gene as a downstream reporter of the bmm promoter. The third-instar larvae of Drosophila showed the GFP signal in all tissues observed and specifically in the salivary gland nucleus. To confirm the relationship between bmm expression and obesity, the effect of oral administration of glucose diets on bmm promoter activity was analyzed. The Drosophila flies given high-glucose diets showed higher lipid contents, indicating the obesity phenotype; this was suggested by a weaker intensity of the GFP signal as well as reduced bmm mRNA expression. These results demonstrated that the transgenic Drosophila model established in this study is useful for screening antiobesity agents. We also report the effects of oral administration of histone deacetylase inhibitors and some vegetables on the bmm promoter activity. PMID:27247940

  4. A Drosophila Model for Screening Antiobesity Agents

    Directory of Open Access Journals (Sweden)

    Tran Thanh Men

    2016-01-01

    Full Text Available Although triacylglycerol, the major component for lipid storage, is essential for normal physiology, its excessive accumulation causes obesity in adipose tissue and is associated with organ dysfunction in nonadipose tissue. Here, we focused on the Drosophila model to develop therapeutics for preventing obesity. The brummer (bmm gene in Drosophila melanogaster is known to be homologous with human adipocyte triglyceride lipase, which is related to the regulation of lipid storage. We established a Drosophila model for monitoring bmm expression by introducing the green fluorescent protein (GFP gene as a downstream reporter of the bmm promoter. The third-instar larvae of Drosophila showed the GFP signal in all tissues observed and specifically in the salivary gland nucleus. To confirm the relationship between bmm expression and obesity, the effect of oral administration of glucose diets on bmm promoter activity was analyzed. The Drosophila flies given high-glucose diets showed higher lipid contents, indicating the obesity phenotype; this was suggested by a weaker intensity of the GFP signal as well as reduced bmm mRNA expression. These results demonstrated that the transgenic Drosophila model established in this study is useful for screening antiobesity agents. We also report the effects of oral administration of histone deacetylase inhibitors and some vegetables on the bmm promoter activity.

  5. ANSTO: Australian Nuclear Science and Technology Organization

    International Nuclear Information System (INIS)

    The Australian Nuclear Science and Technology Organization conducts or is engaged in collaborative research and development in the application of nuclear science and associated technology. Through its Australian radio-isotopes unit, it markets radioisotopes, their products and other services for nuclear medicine industry and research. It also operates national nuclear facilities ( HIFAR and Moata research reactors), promote training, provide advice and disseminates information on nuclear science and technology. The booklet briefly outlines these activities. ills

  6. Australian Politics in a Digital Age

    OpenAIRE

    Chen, Peter John

    2013-01-01

    Information and communications technologies are increasingly important in the Australian political landscape. From the adoption of new forms of electoral campaigning to the use of networking technology to organise social movements, media technology has the potential to radically change the way politics is conducted and experienced in this country. The first comprehensive volume on the impact of digital media on Australian politics, this book examines the way these technologies shape political...

  7. Stress in the Indo-Australian plate

    OpenAIRE

    Cloetingh, S. A. P. L.; R. Wortel

    1986-01-01

    We modelled the state of stress in the Indo-Australian plate in order to investigate quantitatively variations observed in tectonic style. The numerical procedure incorporates the dependence of slab pull and ridge push on the age of the oceanic lithosphere. Estimates are presented for the average net resistive forces at the Himalayan collision zone, the suction force acting on the overriding Indo-Australian plate segment at the Tonga-Kermadec trench and the drag at the base of the lithosphere...

  8. Ownership of Australian Equities and Corporate Bonds

    OpenAIRE

    Susan Black; Joshua Kirkwood

    2010-01-01

    Australian financial and non-financial companies tap capital markets – particularly equity and bond markets – to source funds from households, foreign investors and domestic institutional investors. Foreign investors supply around half of these funds, with institutional investors providing most of the remainder; households’ direct holdings are comparatively modest. During the financial crisis, foreign investors’ appetite for Australian assets remained strong, underpinned by the streng...

  9. An overview of Australian Higher Education

    Institute of Scientific and Technical Information of China (English)

    冯静

    2014-01-01

    This paper makes a brief introduction to Australian higher education in the following aspects such as educational ideas, teaching methods and assessment. The author of this paper holds the opinion that it’s necessary to take an overview of Australian higher education into consideration, if you hope that your study in Australia runs smoothly. In brief, this paper makes an attempt to provide a brief idea of higher education in Australia, especially to those who want to study in Australia for reference.

  10. Spotted wing drosophila, Drosophila suzukii (Matsumura)(Diptera: drosophilidae), trapped with combinations of wines and vinegars

    Science.gov (United States)

    Field trapping experiments evaluated wine and vinegar baits for spotted wing drosophila flies, Drosophila suzukii (Matsumura), and assessed variance in biat attractiveness with wit type, vinegar type, and bait age. A mixture of apple cider vinegar and a Merlot wine attracted more flies than a mixtur...

  11. Publishing and Australian Literature: Crisis, Decline or Transformation?

    Directory of Open Access Journals (Sweden)

    Katherine Bode

    2010-09-01

    Full Text Available The globalisation and consolidation of book publishing is widely seen as having negative consequences for Australian literature. Some commentators argue that this shift is detrimental to Australian literature as a whole; others identify the growth of multinational publishing conglomerates with a specific decline in Australian literary fiction. This article explores both positions, first identifying and investigating trends in Australian novel publication and comparing these to trends in the publication of novels from other countries as well as other Australian-originated literature (specifically, poetry and auto/biography. It then considers the specific case of Australian literary fiction, before looking in detail at the output of large publishers of Australian novels. This analysis reveals a recent decline in Australian novel and poetry titles, but offers a more complex picture of this trend than dominant expressions of nostalgia and alarm about the fate of Australian literature and publishing would suggest.

  12. A highly pleiotropic amino acid polymorphism in the Drosophila insulin receptor contributes to life-history adaptation

    Science.gov (United States)

    Paaby, Annalise B.; Bergland, Alan O.; Behrman, Emily L.; Schmidt, Paul S.

    2016-01-01

    Finding the specific nucleotides that underlie adaptive variation is a major goal in evolutionary biology, but polygenic traits pose a challenge because the complex genotype–phenotype relationship can obscure the effects of individual alleles. However, natural selection working in large wild populations can shift allele frequencies and indicate functional regions of the genome. Previously, we showed that the two most common alleles of a complex amino acid insertion–deletion polymorphism in the Drosophila insulin receptor show independent, parallel clines in frequency across the North American and Australian continents. Here, we report that the cline is stable over at least a five-year period and that the polymorphism also demonstrates temporal shifts in allele frequency concurrent with seasonal change. We tested the alleles for effects on levels of insulin signaling, fecundity, development time, body size, stress tolerance, and life span. We find that the alleles are associated with predictable differences in these traits, consistent with patterns of Drosophila life-history variation across geography that likely reflect adaptation to the heterogeneous climatic environment. These results implicate insulin signaling as a major mediator of life-history adaptation in Drosophila, and suggest that life-history trade-offs can be explained by extensive pleiotropy at a single locus. PMID:25319083

  13. The Australian terrestrial carbon budget

    Directory of Open Access Journals (Sweden)

    V. Haverd

    2013-02-01

    Full Text Available This paper reports a study of the full carbon (C-CO2 budget of the Australian continent, focussing on 1990–2011 in the context of estimates over two centuries. The work is a contribution to the RECCAP (REgional Carbon Cycle Assessment and Processes project, as one of numerous regional studies. In constructing the budget, we estimate the following component carbon fluxes: net primary production (NPP; net ecosystem production (NEP; fire; land use change (LUC; riverine export; dust export; harvest (wood, crop and livestock and fossil fuel emissions (both territorial and non-territorial. Major biospheric fluxes were derived using BIOS2 (Haverd et al., 2012, a fine-spatial-resolution (0.05° offline modelling environment in which predictions of CABLE (Wang et al., 2011, a sophisticated land surface model with carbon cycle, are constrained by multiple observation types. The mean NEP reveals that climate variability and rising CO2 contributed 12 ± 24 (1σ error on mean and 68 ± 15 TgC yr−1, respectively. However these gains were partially offset by fire and LUC (along with other minor fluxes, which caused net losses of 26 ± 4 TgC yr−1 and 18 ± 7 TgC yr−1, respectively. The resultant net biome production (NBP is 36 ± 29 TgC yr−1, in which the largest contributions to uncertainty are NEP, fire and LUC. This NBP offset fossil fuel emissions (95 ± 6 TgC yr−1 by 38 ± 30%. The interannual variability (IAV in the Australian carbon budget exceeds Australia's total carbon emissions by fossil fuel combustion and is dominated by IAV in NEP. Territorial fossil fuel emissions are significantly smaller than the rapidly growing fossil fuel exports: in 2009–2010, Australia exported 2.5 times more carbon in fossil fuels than it emitted by burning fossil fuels.

  14. FlyBase: the Drosophila database. The Flybase Consortium.

    OpenAIRE

    1996-01-01

    FlyBase is a database of genetic and molecular data concerning Drosophila. FlyBase is maintained as a relational database (in Sybase). The scope of FlyBase includes: genes, alleles (and phenotypes), aberrations, pointers to sequence data, clones, stock lists, Drosophila workers and bibliographic references. FlyBase is also available on CD-ROM for Macintosh systems (Encyclopaedia of Drosophila).

  15. Marine biodiversity in the Australian region.

    Directory of Open Access Journals (Sweden)

    Alan J Butler

    Full Text Available The entire Australian marine jurisdictional area, including offshore and sub-Antarctic islands, is considered in this paper. Most records, however, come from the Exclusive Economic Zone (EEZ around the continent of Australia itself. The counts of species have been obtained from four primary databases (the Australian Faunal Directory, Codes for Australian Aquatic Biota, Online Zoological Collections of Australian Museums, and the Australian node of the Ocean Biogeographic Information System, but even these are an underestimate of described species. In addition, some partially completed databases for particular taxonomic groups, and specialized databases (for introduced and threatened species have been used. Experts also provided estimates of the number of known species not yet in the major databases. For only some groups could we obtain an (expert opinion estimate of undiscovered species. The databases provide patchy information about endemism, levels of threat, and introductions. We conclude that there are about 33,000 marine species (mainly animals in the major databases, of which 130 are introduced, 58 listed as threatened and an unknown percentage endemic. An estimated 17,000 more named species are either known from the Australian EEZ but not in the present databases, or potentially occur there. It is crudely estimated that there may be as many as 250,000 species (known and yet to be discovered in the Australian EEZ. For 17 higher taxa, there is sufficient detail for subdivision by Large Marine Domains, for comparison with other National and Regional Implementation Committees of the Census of Marine Life. Taxonomic expertise in Australia is unevenly distributed across taxa, and declining. Comments are given briefly on biodiversity management measures in Australia, including but not limited to marine protected areas.

  16. Development of dendrite polarity in Drosophila neurons

    Directory of Open Access Journals (Sweden)

    Hill Sarah E

    2012-10-01

    Full Text Available Abstract Background Drosophila neurons have dendrites that contain minus-end-out microtubules. This microtubule arrangement is different from that of cultured mammalian neurons, which have mixed polarity microtubules in dendrites. Results To determine whether Drosophila and mammalian dendrites have a common microtubule organization during development, we analyzed microtubule polarity in Drosophila dendritic arborization neuron dendrites at different stages of outgrowth from the cell body in vivo. As dendrites initially extended, they contained mixed polarity microtubules, like mammalian neurons developing in culture. Over a period of several days this mixed microtubule array gradually matured to a minus-end-out array. To determine whether features characteristic of dendrites were localized before uniform polarity was attained, we analyzed dendritic markers as dendrites developed. In all cases the markers took on their characteristic distribution while dendrites had mixed polarity. An axonal marker was also quite well excluded from dendrites throughout development, although this was perhaps more efficient in mature neurons. To confirm that dendrite character could be acquired in Drosophila while microtubules were mixed, we genetically disrupted uniform dendritic microtubule organization. Dendritic markers also localized correctly in this case. Conclusions We conclude that developing Drosophila dendrites initially have mixed microtubule polarity. Over time they mature to uniform microtubule polarity. Dendrite identity is established before the mature microtubule arrangement is attained, during the period of mixed microtubule polarity.

  17. Genomic Evidence for Adaptive Inversion Clines in Drosophila melanogaster.

    Science.gov (United States)

    Kapun, Martin; Fabian, Daniel K; Goudet, Jérôme; Flatt, Thomas

    2016-05-01

    Clines in chromosomal inversion polymorphisms-presumably driven by climatic gradients-are common but there is surprisingly little evidence for selection acting on them. Here we address this long-standing issue in Drosophila melanogaster by using diagnostic single nucleotide polymorphism (SNP) markers to estimate inversion frequencies from 28 whole-genome Pool-seq samples collected from 10 populations along the North American east coast. Inversions In(3L)P, In(3R)Mo, and In(3R)Payne showed clear latitudinal clines, and for In(2L)t, In(2R)NS, and In(3R)Payne the steepness of the clinal slopes changed between summer and fall. Consistent with an effect of seasonality on inversion frequencies, we detected small but stable seasonal fluctuations of In(2R)NS and In(3R)Payne in a temperate Pennsylvanian population over 4 years. In support of spatially varying selection, we observed that the cline in In(3R)Payne has remained stable for >40 years and that the frequencies of In(2L)t and In(3R)Payne are strongly correlated with climatic factors that vary latitudinally, independent of population structure. To test whether these patterns are adaptive, we compared the amount of genetic differentiation of inversions versus neutral SNPs and found that the clines in In(2L)t and In(3R)Payne are maintained nonneutrally and independent of admixture. We also identified numerous clinal inversion-associated SNPs, many of which exhibit parallel differentiation along the Australian cline and reside in genes known to affect fitness-related traits. Together, our results provide strong evidence that inversion clines are maintained by spatially-and perhaps also temporally-varying selection. We interpret our data in light of current hypotheses about how inversions are established and maintained. PMID:26796550

  18. Representations of the Japanese in Contemporary Australian Literature and Film

    OpenAIRE

    Erika Smith

    2008-01-01

    The objective of this article is to investigate general contemporary Australian perceptions of the Japanese. I will do this by exploring how Australian contemporary literature (2006- 2007) and Australian contemporary film (1997-2007) depicts Japanese characters. By analysing the representation of the Japanese characters in these areas I will attempt to gather a broad understanding of how Australians represent, perceive and identify the Japanese today.

  19. An expanded prescribing role for pharmacists - an Australian perspective

    OpenAIRE

    Kreshnik Hoti; Jeffery Hughes; Bruce Sunderland

    2011-01-01

    Expanded pharmacist prescribing is a new professional practice area for pharmacists. Currently, Australian pharmacists’ prescribing role is limited to over-the-counter medications. This review aims to identify Australian studies involving the area of expanded pharmacist prescribing. Australian studies exploring the issues of pharmacist prescribing were identified and considered in the context of its implementation internationally. Australian studies have mainly focused on the attitudes of com...

  20. Data Convergence - An Australian Perspective

    Science.gov (United States)

    Allen, S. S.; Howell, B.

    2012-12-01

    Coupled numerical physical, biogeochemical and sediment models are increasingly being used as integrators to help understand the cumulative or far field effects of change in the coastal environment. This reliance on modeling has forced observations to be delivered as data streams ingestible by modeling frameworks. This has made it easier to create near real-time or forecasting models than to try to recreate the past, and has lead in turn to the conversion of historical data into data streams to allow them to be ingested by the same frameworks. The model and observation frameworks under development within Australia's Commonwealth and Industrial Research Organisation (CSIRO) are now feeding into the Australian Ocean Data Network's (AODN's) MARine Virtual Laboratory (MARVL) . The sensor, or data stream, brokering solution is centred around the "message" and all data flowing through the gateway is wrapped as a message. Messages consist of a topic and a data object and their routing through the gateway to pre-processors and listeners is determined by the topic. The Sensor Message Gateway (SMG) method is allowing data from different sensors measuring the same thing but with different temporal resolutions, units or spatial coverage to be ingested or visualized seamlessly. At the same time the model output as a virtual sensor is being explored, this again being enabled by the SMG. It is only for two way communications with sensor that rigorous adherence to standards is needed, by accepting existing data in less than ideal formats, but exposing them though the SMG we can move a step closer to the Internet Of Things by creating an Internet of Industries where each vested interest can continue with business as usual, contribute to data convergence and adopt more open standards when investment seems appropriate to that sector or business.Architecture Overview

  1. Apoptosis in Drosophila: which role for mitochondria?

    Science.gov (United States)

    Clavier, Amandine; Rincheval-Arnold, Aurore; Colin, Jessie; Mignotte, Bernard; Guénal, Isabelle

    2016-03-01

    It is now well established that the mitochondrion is a central regulator of mammalian cell apoptosis. However, the importance of this organelle in non-mammalian apoptosis has long been regarded as minor, mainly because of the absence of a crucial role for cytochrome c in caspase activation. Recent results indicate that the control of caspase activation and cell death in Drosophila occurs at the mitochondrial level. Numerous proteins, including RHG proteins and proteins of the Bcl-2 family that are key regulators of Drosophila apoptosis, constitutively or transiently localize in mitochondria. These proteins participate in the cell death process at different levels such as degradation of Diap1, a Drosophila IAP, production of mitochondrial reactive oxygen species or stimulation of the mitochondrial fission machinery. Here, we review these mitochondrial events that might have their counterpart in human. PMID:26679112

  2. Live cell imaging in Drosophila melanogaster.

    Science.gov (United States)

    Parton, Richard M; Vallés, Ana Maria; Dobbie, Ian M; Davis, Ilan

    2010-04-01

    Although many of the techniques of live cell imaging in Drosophila melanogaster are also used by the greater community of cell biologists working on other model systems, studying living fly tissues presents unique difficulties with regard to keeping the cells alive, introducing fluorescent probes, and imaging through thick, hazy cytoplasm. This article outlines the major tissue types amenable to study by time-lapse cinematography and different methods for keeping the cells alive. It describes various imaging and associated techniques best suited to following changes in the distribution of fluorescently labeled molecules in real time in these tissues. Imaging, in general, is a rapidly developing discipline, and recent advances in imaging technology are able to greatly extend what can be achieved with live cell imaging of Drosophila tissues. As far as possible, this article includes the latest technical developments and discusses likely future developments in imaging methods that could have an impact on research using Drosophila. PMID:20360379

  3. Topics from Australian Conferences on Teaching Statistics

    CERN Document Server

    Phillips, Brian; Martin, Michael

    2014-01-01

    The first OZCOTS conference in 1998 was inspired by papers contributed by Australians to the 5th International Conference on Teaching Statistics. In 2008, as part of the program of one of the first National Senior Teaching Fellowships, the 6th OZCOTS was held in conjunction with the Australian Statistical Conference, with Fellowship keynotes and contributed papers, optional refereeing and proceedings. This venture was so successful that the 7th and 8th OZCOTS were similarly run, conjoined with Australian Statistical Conferences in 2010 and 2012. Authors of papers from these OZCOTS conferences were invited to develop chapters for refereeing and inclusion in this volume. There are sections on keynote topics, undergraduate curriculum and learning, professional development, postgraduate learning, and papers from OZCOTS 2012. Because OZCOTS aim to unite statisticians and statistics educators, the approaches this volume takes are immediately relevant to all who have a vested interest in good teaching practices. Glo...

  4. Time Travel: Australian Tourists and Britain's Past

    Directory of Open Access Journals (Sweden)

    Richard White

    2012-06-01

    Full Text Available Across the twentieth century, Britain drew more Australian tourists for longer and more intense experiences than anywhere else, though as early as the 1970s Asia was attracting more Australians than Europe. They found much to admire and to deprecate in Britain but above all they were seduced by Britain’s past, or what they imagined it to be. This paper examines the Australian experience of history in Britain, their admiration for notions of tradition, for an unchanging village life, for fading imperial glory, for sheer antiquity. Some looked for their own ancestors and family but most were satisfied to have their school lessons and imaginative reading validated by being there. The response they had to British history was an intensely emotional one: this article argues that it was a result not of imperial sentiment but of a desire for a deep and meaningful past.

  5. Aboriginal Agency and Marginalisation in Australian Society

    Directory of Open Access Journals (Sweden)

    Terry Moore

    2014-09-01

    Full Text Available It is often argued that while state rhetoric may be inclusionary, policies and practices may be exclusionary. This can imply that the power to include rests only with the state. In some ways, the implication is valid in respect of Aboriginal Australians. For instance, the Australian state has gained control of Aboriginal inclusion via a singular, bounded category and Aboriginal ideal type. However, the implication is also limited in their respect. Aborigines are abject but also agents in their relationship with the wider society. Their politics contributes to the construction of the very category and type that governs them, and presses individuals to resist state inclusionary efforts. Aboriginal political elites police the performance of an Aboriginality dominated by notions of difference and resistance. The combined processes of governance act to deny Aborigines the potential of being both Aboriginal and Australian, being different and belonging. They maintain Aborigines’ marginality.

  6. Drama in the Australian National Curriculum: Decisions, Tensions and Uncertainties

    Science.gov (United States)

    Stinson, Madonna; Saunders, John Nicholas

    2016-01-01

    In September 2015, the Australian Federal Government endorsed the final version of the Australian Curriculum arts framework a document resulting from nearly seven years of consultation and development. "The Australian Curriculum: The Arts Version 8.0" comprises five subjects: dance, drama, media arts, music and visual arts. This article…

  7. Modelling Choice: Factors Influencing Modes of Delivery in Australian Universities

    Science.gov (United States)

    Smith, Andrew; Ling, Peter; Hill, Doug

    2008-01-01

    This paper reports the findings of a study of Multiple Modes of Delivery in Australian universities that was commissioned by Australian Universities Teaching Committee over the period 2001-2004. The project examined and described the various means of educational delivery deployed by Australian universities. It identified the pedagogical,…

  8. Australian clinical guidelines for radiological event

    International Nuclear Information System (INIS)

    The Australian Health Protection Committee oversees national health protection priorities in: communicable disease outbreaks; chemical, biological or radiological incidents; mass casualty incidents; and deployment of Australian health teams overseas. The Australian Clinical Guidelines for a Radiological Event to complement existing national guidelines on chemical agents, anthrax, and smallpox. Other prompts included the need to revise the ARPANSA Guidance Manual, Medical Management of Individuals Involved in Radiation Accidents, 2000, and the requirement for specific therapeutic information regarding the indications and use of radiological decorporation agents held as part of the National Medical Stockpile. Matters identified by clinicians requiring specific guidance included: basic understanding of radiation; an approach to dose assessment; specific thresholds for initiating decorporation and other therapy; the role of gastric lavage, as contemporary practice considers this ineffective for other toxicological indications. rationale for, and detailed description of pulmonary lavage; advice on prenatal exposure to radiation; protocols for biodosimetry and other laboratory analysis. The objective was to produce a plain language guidance document for Australian clinicians on the diagnosis and management of radiation injury. It was to be based on evaluation of existing Australian documents, a literature review and consultation of appropriate specialists. Content areas included human health effects of radiation, scenario-based risk assessment and risk management, pre-hospital and hospital systems of care, management of specific injury types, radionuclide pathophysiology and decorporation protocols, biodosimetry options, individual psychological support 'and public health information, and Australian responsibilities under the Radiation Emergency Medical Preparedness and Assistance Network, of the World Health Organization. The range of resources utilised in preparing the

  9. Astronomical Symbolism in Australian Aboriginal Rock Art

    CERN Document Server

    Norris, Ray P

    2010-01-01

    Traditional Aboriginal Australian cultures include a significant astronomical component, perpetuated through oral tradition and ceremony. This knowledge has practical navigational and calendrical functions, and sometimes extends to a deep understanding of the motion of objects in the sky. Here we explore whether this astronomical tradition is reflected in the rock art of Aboriginal Australians. We find several plausible examples of depictions of astronomical figures and symbols, and also evidence that astronomical observations were used to set out stone arrangements. However, we recognise that the case is not yet strong enough to make an unequivocal statement, and describe our plans for further research.

  10. Western Australian food security project

    Directory of Open Access Journals (Sweden)

    Maycock Bruce

    2007-08-01

    Full Text Available Abstract Background The aim of the Western Australian (WA Food Security Project was to conduct a preliminary investigation into issues relating to food security in one region within the Perth metropolitan area in Western Australia. The first phase of the project involved a food audit in one lower income area that was typical of the region, to identify the range, variety and availability of foods in the region. Methods A comprehensive food audit survey was provided to all food outlet owners/operators in one lower socio-economic region within the City of Mandurah (n = 132 outlets. The purpose of the survey was to investigate the range, variety and availability of foods in the Mandurah region as well as examining specific in-store characteristics such as the types of clientele and in-store promotions offered. Surveys were competed for 99 outlets (response rate = 75%. Results The range of foods available were predominantly pre-prepared with more than half of the outlets pre-preparing the majority of their food. Sandwiches and rolls were the most popular items sold in the outlets surveyed (n = 51 outlets followed by pastries such as pies, sausage rolls and pasties (n = 33 outlets. Outlets considered their healthiest food options were sandwiches or rolls (n = 51 outlets, salads (n- = 50 outlets, fruit and vegetables (n = 40 outlets, seafood (n = 27 outlets, meats such as chicken (n = 26 outlets and hot foods such as curries, soups or quiches (n = 23 outlets. The majority of outlets surveyed considered pre-prepared food including sandwiches, rolls and salads, as healthy food options regardless of the content of the filling or dressings used. Few outlets (n = 28% offered a choice of bread type other than white or wholemeal. High fat pastries and dressings were popular client choices (n = 77% as were carbonated drinks (n = 88% and flavoured milks (n = 46%. Conclusion These findings clearly indicate the need for further investigation of the impact of

  11. Drosophila bitter taste(s

    Directory of Open Access Journals (Sweden)

    Alice eFrench

    2015-11-01

    Full Text Available Most animals possess taste receptors neurons detecting potentially noxious compounds. In humans, the ligands which activate these neurons define a sensory space called bitter. By extension, this term has been used in animals and insects to define molecules which induce aversive responses. In this review, based on our observations carried out in Drosophila, we examine how bitter compounds are detected and if the activation of bitter-sensitive neurons respond only to molecules bitter to humans. Like most animals, flies detect bitter chemicals through a specific population of taste neurons, distinct from those responding to sugars or to other modalities. Activating bitter-sensitive taste neurons induce aversive reactions and inhibits feeding. Bitter molecules also contribute to the suppression of sugar-neuron responses and can lead to a complete inhibition of the responses to sugar at the periphery. Since some bitter molecules activate bitter-sensitive neurons and some inhibit sugar detection, bitter molecules are represented by two sensory spaces which are only partially congruent. In addition to molecules which impact feeding, we recently discovered that the activation of bitter-sensitive neurons also induces grooming. Bitter-sensitive neurons of the wings and of the legs can sense chemicals from the gram negative bacteria, Escherichia coli, thus adding another biological function to these receptors. Bitter-sensitive neurons of the proboscis also respond to inhibitory pheromones such as 7-tricosene. Activating these neurons by bitter molecules in the context of sexual encounter inhibits courting and sexual reproduction, while activating these neurons with 7-tricosene in a feeding context will inhibit feeding. The picture that emerges from these observations is that the taste system is composed of detectors which monitor different categories of ligands, which facilitate or inhibit behaviors depending on the context (feeding, sexual reproduction

  12. Automated measurement of Drosophila wings

    Directory of Open Access Journals (Sweden)

    Mezey Jason

    2003-12-01

    Full Text Available Abstract Background Many studies in evolutionary biology and genetics are limited by the rate at which phenotypic information can be acquired. The wings of Drosophila species are a favorable target for automated analysis because of the many interesting questions in evolution and development that can be addressed with them, and because of their simple structure. Results We have developed an automated image analysis system (WINGMACHINE that measures the positions of all the veins and the edges of the wing blade of Drosophilid flies. A video image is obtained with the aid of a simple suction device that immobilizes the wing of a live fly. Low-level processing is used to find the major intersections of the veins. High-level processing then optimizes the fit of an a priori B-spline model of wing shape. WINGMACHINE allows the measurement of 1 wing per minute, including handling, imaging, analysis, and data editing. The repeatabilities of 12 vein intersections averaged 86% in a sample of flies of the same species and sex. Comparison of 2400 wings of 25 Drosophilid species shows that wing shape is quite conservative within the group, but that almost all taxa are diagnosably different from one another. Wing shape retains some phylogenetic structure, although some species have shapes very different from closely related species. The WINGMACHINE system facilitates artificial selection experiments on complex aspects of wing shape. We selected on an index which is a function of 14 separate measurements of each wing. After 14 generations, we achieved a 15 S.D. difference between up and down-selected treatments. Conclusion WINGMACHINE enables rapid, highly repeatable measurements of wings in the family Drosophilidae. Our approach to image analysis may be applicable to a variety of biological objects that can be represented as a framework of connected lines.

  13. The Asian currency crisis and the Australian health industry.

    Science.gov (United States)

    Barraclough, S

    1998-01-01

    This article identifies linkages between the Australian health industry and the global economy. It discusses some of the consequences of the Asian currency crisis of 1997-98 for the Australian economy and health industry, with special emphasis upon exports. Devaluation of the Australian dollar will increase the cost of most pharmaceutical and medical imports, but may offer competitive advantages to some Australian exporters. The nascent engagement with Asia of many health industry enterprises is likely to be stifled. It is therefore important for Australian governments, as well as the Australian health industry, to provide intelligence and encouragement to those enterprises that wish to continue their engagement with Asia or resume it when economic equilibrium returns. Markets throughout the world must also be further developed. The crisis may therefore provide the stimulus for re-thinking and re-stating Australian health export policy. PMID:10537568

  14. The Asian currency crisis and the Australian health industry.

    Science.gov (United States)

    Barraclough, S

    1998-01-01

    This article identifies linkages between the Australian health industry and the global economy. It discusses some of the consequences of the Asian currency crisis of 1997-98 for the Australian economy and health industry, with special emphasis upon exports. Devaluation of the Australian dollar will increase the cost of most pharmaceutical and medical imports, but may offer competitive advantages to some Australian exporters. The nascent engagement with Asia of many health industry enterprises is likely to be stifled. It is therefore important for Australian governments, as well as the Australian health industry, to provide intelligence and encouragement to those enterprises that wish to continue their engagement with Asia or resume it when economic equilibrium returns. Markets throughout the world must also be further developed. The crisis may therefore provide the stimulus for re-thinking and re-stating Australian health export policy.

  15. Australian Study Cites Low English Standards

    Science.gov (United States)

    Cohen, David

    2007-01-01

    This article reports the findings of a study showing that one-third of all foreign students who studied at Australian universities speak English so poorly that they should never have been granted visas to study in the country in the first place. The study, by Robert Birrell, director of the Centre for Population and Urban Research at Australia's…

  16. Does Academic Work Make Australian Academics Happy?

    Science.gov (United States)

    Duncan, Roderick; Tilbrook, Kerry; Krivokapic-Skoko, Branka

    2015-01-01

    Happiness research is a rapidly-growing area in social psychology and has emphasised the link between happiness and workplace productivity and creativity for knowledge workers. Recent articles in this journal have raised concerns about the level of happiness and engagement of Australian academics with their work, however there is little research…

  17. Demands of Training: Australian Tourism and Hospitality.

    Science.gov (United States)

    Freeland, Brett

    Qualitative research was conducted as part of a four-industry project studying operation of training markets, one of which was Australian tourism and hospitality (T&H). Semi-structured interviews were conducted with 23 individuals representing stakeholder groups. Interviews were conducted across Queensland, Victoria, and South Australia and…

  18. Exporting Australian Educational Services to China

    Science.gov (United States)

    Kendall, Timothy

    2004-01-01

    This article examines the deregulation of the overseas student sector that took place in Australia during the mid-1980s. It focuses specifically upon the short-term English- language courses that were sold to students from the People's Republic of China. The article suggests that the Hawke government's policy of encouraging Australian language…

  19. Anglo-Australian Observatory August 2009 newsletter

    CERN Document Server

    Dobbie, Paul

    2009-01-01

    The August 2009 edition of the AAO newsletter contains articles on observations of the lunar impact of the Kaguya satellite, mapping the ISM towards Omega Centauri, early results from the Anglo-Australian Rocky Planet search, details of a new AAOmega observing mode, the new telescope control system and a number of regular features.

  20. Making Space for Multilingualism in Australian Schooling

    Science.gov (United States)

    Turner, Marianne; Cross, Russell

    2016-01-01

    In this article we introduce the special issue: Language(s) across the curriculum in Australian schools. The special issue includes a focus on English as an additional language in mainstream classes, Indigenous education, heritage languages and foreign languages, and we give background to these different--though frequently overlapping--contexts.…

  1. Financial Management and Young Australian Workers

    Science.gov (United States)

    Dowling, Nicki; Hoiles, Lauren; Corney, Tim; Clark, David

    2008-01-01

    In two studies of young Australian workers, participants generally displayed positive attitudes towards financial management practices; however, a substantial proportion failed to display positive financial management practices, experienced financial problems and dissatisfaction, and reported low rates of seeking financial assistance, particularly…

  2. Developments in Australian Agricultural and Related Education

    Science.gov (United States)

    McSweeney, Peter; Rayner, John

    2011-01-01

    While the calm waters metaphor might explain the changes navigated by Australian agricultural education through most of its history, the last 20 or so years have been very turbulent. Now, the new millennium sees agricultural education in both Australia and the Western world facing a different and less certain future. This paper analyses some of…

  3. Australian Education Journals: Quantitative and Qualitative Indicators

    Science.gov (United States)

    Haddow, Gaby; Genoni, Paul

    2009-01-01

    This paper reports on a study that applied citation-based measurements to Australian education journals. Citations data were drawn from two sources, Web of Science and Scopus, and these data were used to calculate each journal's impact factor, "h"-index, and diffusion factor. The rankings resulting from these analyses were compared with draft…

  4. Exposures to patients in Australian radiological practice

    Energy Technology Data Exchange (ETDEWEB)

    Paix, D. (South Australian Inst. of Tech., Adelaide)

    1983-11-01

    The findings of a 1980 Australian Radiation Laboratory study of genetic and bone-marrow doses to the population from medical, dental and chiropractic uses of ionising radiation are discussed. Attention is drawn to the large variability in patient exposure: maximum values were from five to eleven times greater than the means.

  5. Indigenous Australian Artworks in Intercultural Contact Zones

    OpenAIRE

    Eleanore Wildurger

    2009-01-01

    This article comments on Indigenous Australian art from an intercultural perspective. The painting Bush Tomato Dreaming (1998), by the Anmatyerre artist Lucy Ngwarai Kunoth serves as model case for my arg ument that art expresses existential social knowledge. In consequence, I wil l argue that social theory and art theory together provide tools for intercultural und erstanding and competence.

  6. Dipole vortices in the Great Australian Bight

    DEFF Research Database (Denmark)

    Cresswell, George R.; Lund-Hansen, Lars C.; Nielsen, Morten Holtegaard

    2015-01-01

    Shipboard measurements from late 2006 made by the Danish Galathea 3 Expedition and satellite sea surface temperature images revealed a chain of cool and warm mushroom' dipole vortices that mixed warm, salty, oxygen-poor waters on and near the continental shelf of the Great Australian Bight (GAB...

  7. Marketing in the Australian Higher Education Sector

    Science.gov (United States)

    Favaloro, Chrissa

    2015-01-01

    This article examines domestic marketing in the Australian higher education sector, specifically, the marketing investment patterns of universities and their levels of student growth as a return on marketing investment. Marketing expenditure by universities has risen 23 per cent in the five years to 2013, with several institutions allocating in…

  8. Indigenous Australian art in intercultural contact zones

    Directory of Open Access Journals (Sweden)

    Eleonore Wildburger

    2009-06-01

    Full Text Available This article comments on Indigenous Australian art from an intercultural perspective. The painting Bush Tomato Dreaming (1998, by the Anmatyerre artist Lucy Ngwarai Kunoth serves as model case for my argument that art expresses existential social knowledge. In consequence, I will argue that social theory and art theory together provide tools for intercultural understanding and competence.

  9. Learning Choices, Older Australians and Active Ageing

    Science.gov (United States)

    Boulton-Lewis, Gillian M.; Buys, Laurie

    2015-01-01

    This paper reports on the findings of qualitative, semistructured interviews conducted with 40 older Australian participants who either did or did not engage in organized learning. Phenomenology was used to guide the interviews and analysis to explore the lived learning experiences and perspectives of these older people. Their experiences of…

  10. Sustainability in the Australian Curriculum: Geography

    Science.gov (United States)

    Maude, Alaric

    2014-01-01

    "Sustainability" is one of the seven major concepts in the geography curriculum. It is also one of the three cross-curriculum priorities in the Australian curriculum, together with Asia and Australia's engagement with Asia, and Aboriginal and Torres Strait Islander histories and cultures. This paper describes how the concept is explained…

  11. Commercial Activities and Copyright in Australian Universities

    Science.gov (United States)

    Shelly, Marita

    2008-01-01

    With government funding for most Australian universities below 60% and falling a major strategic emphasis for universities has been on securing other sources of operating revenue, including commercial opportunities and partnerships. The implication of increasing commercial activities such as non-award and tailored professional programmes, contract…

  12. Stress in the Indo-Australian plate

    NARCIS (Netherlands)

    Cloetingh, S.A.P.L.; Wortel, R.

    1986-01-01

    We modelled the state of stress in the Indo-Australian plate in order to investigate quantitatively variations observed in tectonic style. The numerical procedure incorporates the dependence of slab pull and ridge push on the age of the oceanic lithosphere. Estimates are presented for the average ne

  13. Is achievement in Australian chemistry gender based?

    Science.gov (United States)

    Beard, John; Fogliani, Charles; Owens, Chris; Wilson, Audrey

    1993-12-01

    This paper compares the performances of female and male secondary students in the 1991 and 1992 Australian National Chemistry Quizzes. Male students consistently achieved a higher mean score in all Year groups (7 to 12), even though the numbers of female and male entrants were approximately equal. Implications for class tests and assessment tasks are addressed.

  14. Professional Standards for Australian Special Education Teachers

    Science.gov (United States)

    Dempsey, Ian; Dally, Kerry

    2014-01-01

    Although professional standards for Australian teachers were developed several years ago, this country is yet to develop such standards for special education teachers. The lack of standards for the special education profession is associated with the absence of a consistent process of accreditation in Australia and a lack of clarity in the pathways…

  15. Education for Sustainability and the Australian Curriculum

    Science.gov (United States)

    Kennelly, Julie; Taylor, Neil; Serow, Pep

    2011-01-01

    A national curriculum is presently being developed in Australia with implementation due during 2014. Associated standards for the accreditation of teachers and for teacher education providers have been prepared with the standards describing skills and attributes that teachers are expected to attain. The developing Australian Curriculum, along with…

  16. Box Plots in the Australian Curriculum

    Science.gov (United States)

    Watson, Jane M.

    2012-01-01

    This article compares the definition of "box plot" as used in the "Australian Curriculum: Mathematics" with other definitions used in the education community; describes the difficulties students experience when dealing with box plots; and discusses the elaboration that is necessary to enable teachers to develop the knowledge necessary to use them…

  17. Is There Cultural Safety in Australian Universities?

    Science.gov (United States)

    Rochecouste, Judith; Oliver, Rhonda; Bennell, Debra

    2014-01-01

    This paper examines the cultural safety offered to Australian Aboriginal and Torres Strait Islander students within their university environments. In the context of this paper, cultural safety includes cultural competency, as recently subscribed by Universities Australia, and "extends beyond (to) cultural awareness and cultural…

  18. Cognitive and Social Play of Australian Preschoolers.

    Science.gov (United States)

    Wyver, Shirley R.; Spence, Susan H.

    1995-01-01

    Observed behaviors of 37 female and 23 male Australian preschoolers. Found that only 20% engaged in thematic pretend play (linked to perspective taking, language development, impulse control, divergent problem solving) whereas 24% used cooperative social play (linked to divergent problem solving). Results suggest need for assistance in the…

  19. Scholarly Communication Costs in Australian Higher Education

    Science.gov (United States)

    Houghton, John W

    2008-01-01

    This paper reports on the development and application of a model used to estimate the costs of scholarly communication (i.e. scholarly publishing and related activities) in Australian higher education. A systems perspective was used to frame a review of the literature on the costs involved in the entire scholarly communication value chain and…

  20. Brain drain threat to Australian science

    Science.gov (United States)

    Banks, Michael

    2011-11-01

    Around half of all academics in Australia intend to retire, move to an overseas university or leave Australian higher education within the next 10 years, according to a survey of more than 5500 researchers based at 20 universities in the country.

  1. Characterization of novel microsatellites from Drosophila transversa.

    Science.gov (United States)

    Räisänen, L; Roininen, E; Liimatainen, J O

    2009-03-01

    We investigated a partial genomic library of Drosophila transversa for microsatellites and developed 12 markers for genetic analyses. This is the first time that microsatellite primers from the quinaria species group have been described. Four loci were cross-amplified in D. phalerata. Nine out of the 12 microsatellite markers developed are likely to be on the X chromosome. PMID:21564716

  2. Drosophila lifespan enhancement by exogenous bacteria

    OpenAIRE

    Brummel, Ted; Ching, Alisa; Seroude, Laurent; Simon, Anne F.; Benzer, Seymour

    2004-01-01

    We researched the lifespan of Drosophila under axenic conditions compared with customary procedure. The experiments revealed that the presence of bacteria during the first week of adult life can enhance lifespan, despite unchanged food intake. Later in life, the presence of bacteria can reduce lifespan. Certain long-lived mutants react in different ways, indicating an interplay between bacteria and longevity-enhancing genes.

  3. Polarity and intracellular compartmentalization of Drosophila neurons

    Directory of Open Access Journals (Sweden)

    Henner Astra L

    2007-04-01

    Full Text Available Abstract Background Proper neuronal function depends on forming three primary subcellular compartments: axons, dendrites, and soma. Each compartment has a specialized function (the axon to send information, dendrites to receive information, and the soma is where most cellular components are produced. In mammalian neurons, each primary compartment has distinctive molecular and morphological features, as well as smaller domains, such as the axon initial segment, that have more specialized functions. How neuronal subcellular compartments are established and maintained is not well understood. Genetic studies in Drosophila have provided insight into other areas of neurobiology, but it is not known whether flies are a good system in which to study neuronal polarity as a comprehensive analysis of Drosophila neuronal subcellular organization has not been performed. Results Here we use new and previously characterized markers to examine Drosophila neuronal compartments. We find that: axons and dendrites can accumulate different microtubule-binding proteins; protein synthesis machinery is concentrated in the cell body; pre- and post-synaptic sites localize to distinct regions of the neuron; and specializations similar to the initial segment are present. In addition, we track EB1-GFP dynamics and determine microtubules in axons and dendrites have opposite polarity. Conclusion We conclude that Drosophila will be a powerful system to study the establishment and maintenance of neuronal compartments.

  4. Radiation effects on the drosophila melanogaster genoma

    International Nuclear Information System (INIS)

    When DNA of living beings has been damaged, the cells show different responses depending on their physiological state. Repair mechanisms can be classified into two groups: constitutive which are always present in the cells and inductible, which must be stimulated to show themselves. It is suggested that a repair mechanism exists in the drosophila ovules which act upon the damage present in mature spermatozoids. Our aim is to verify whether or not a radiation dosis applied to the female drosophila will modify the frequency of individuals which have lost the paternal sex chromosomes. YW/YW virgin females and XEZ males and fbb-/bS Y y+ y were mated for two days in order to collect radiation treated spermatozoids. The results were consistent as to the parameters being evaluated and lead one to suppose that the radiation applied to the female drosophila produced some changes in the ovule metabolism which reduced the frequency of individuals with lost chromosomes. It is believed that ionizing radiation interferes with the repair mechanisms that are existent and constitutive, retarding and hindering the restoration of chromosome fragments and this brings about death of the zygote or death of the eggs which lessens the frequencies of individuals carriers of chromosomic aberrations. Ionizing radiations applied to the female drosophila modifies the frequency of loss of patternal chromosomes and comes about when the radiation dose to the female is 700 rad. (Author)

  5. Biological effects of radon in Drosophila

    International Nuclear Information System (INIS)

    The main objective of this investigation, is to study the biological effects of the Radon-222 at low dose in 'Drosophila melanogaster'. It is necessary to mention that these effects will analyze from the genetic point of view for: 1) To evaluate in which form the Radon-222 to low dose it influences in some genetic components of the adaptation in Drosophila, such as: fecundity, viability egg-adult and sex proportion. 2) To evaluate which is the genetic effect that induces the Radon to low dose by means of the SMART technique in Drosophila melanogaster, and this way to try of to identify which is the possible mechanism that causes the genetic damage to somatic level. The carried out investigation was divided in three stages: 1. Tests to the vacuum resistance. 2. Test of somatic mutation, and 3. Determination of the presence of radon daughters on the adult of Drosophila. It is necessary to point out that all the experiments were made by triplicate and in each one of them was placed detectors in preset places. Those obtained results are presented inside the 4 charts included in the present work. (Author)

  6. Organization of descending neurons in Drosophila melanogaster.

    Science.gov (United States)

    Hsu, Cynthia T; Bhandawat, Vikas

    2016-01-01

    Neural processing in the brain controls behavior through descending neurons (DNs) - neurons which carry signals from the brain to the spinal cord (or thoracic ganglia in insects). Because DNs arise from multiple circuits in the brain, the numerical simplicity and availability of genetic tools make Drosophila a tractable model for understanding descending motor control. As a first step towards a comprehensive study of descending motor control, here we estimate the number and distribution of DNs in the Drosophila brain. We labeled DNs by backfilling them with dextran dye applied to the neck connective and estimated that there are ~1100 DNs distributed in 6 clusters in Drosophila. To assess the distribution of DNs by neurotransmitters, we labeled DNs in flies in which neurons expressing the major neurotransmitters were also labeled. We found DNs belonging to every neurotransmitter class we tested: acetylcholine, GABA, glutamate, serotonin, dopamine and octopamine. Both the major excitatory neurotransmitter (acetylcholine) and the major inhibitory neurotransmitter (GABA) are employed equally; this stands in contrast to vertebrate DNs which are predominantly excitatory. By comparing the distribution of DNs in Drosophila to those reported previously in other insects, we conclude that the organization of DNs in insects is highly conserved. PMID:26837716

  7. Optogenetic pacing in Drosophila melanogaster (Conference Presentation)

    Science.gov (United States)

    Alex, Aneesh; Li, Airong; Men, Jing; Jerwick, Jason; Tanzi, Rudolph E.; Zhou, Chao

    2016-03-01

    A non-invasive, contact-less cardiac pacing technology can be a powerful tool in basic cardiac research and in clinics. Currently, electrical pacing is the gold standard for cardiac pacing. Although highly effective in controlling the cardiac function, the invasive nature, non-specificity to cardiac tissues and possible tissue damage limits its capabilities. Optical pacing of heart is a promising alternative, which is non-invasive and more specific, has high spatial and temporal precision, and avoids shortcomings in electrical stimulation. Optical coherence tomography has been proved to be an effective technique in non-invasive imaging in vivo with ultrahigh resolution and imaging speed. In the last several years, non-invasive specific optical pacing in animal hearts has been reported in quail, zebrafish, and rabbit models. However, Drosophila Melanogaster, which is a significant model with orthologs of 75% of human disease genes, has rarely been studied concerning their optical pacing in heart. Here, we combined optogenetic control of Drosophila heartbeat with optical coherence microscopy (OCM) technique for the first time. The light-gated cation channel, channelrhodopsin-2 (ChR2) was specifically expressed by transgene as a pacemaker in drosophila heart. By stimulating the pacemaker with 472 nm pulsed laser light at different frequencies, we achieved non-invasive and more specific optical control of the Drosophila heart rhythm, which demonstrates the wide potential of optical pacing for studying cardiac dynamics and development. Imaging capability of our customized OCM system was also involved to observe the pacing effect visually. No tissue damage was found after long exposure to laser pulses, which proved the safety of optogenetic control of Drosophila heart.

  8. Organization and evolution of Drosophila terminin: similarities and differences between Drosophila and human telomeres

    Directory of Open Access Journals (Sweden)

    Grazia Daniela Raffa

    2013-05-01

    Full Text Available Drosophila lacks telomerase and fly telomeres are elongated by occasional transposition of three specialized retroelements. Drosophila telomeres do not terminate with GC-rich repeats and are assembled independently of the sequence of chromosome ends. Recent work has shown that Drosophila telomeres are capped by the terminin complex, which includes the fast-evolving proteins HOAP, HipHop, Moi and Ver. These proteins are not conserves outside Drosophilidae and localize and function exclusively at telomeres, protecting them from fusion events. Other proteins required to prevent end-to-end fusion in flies include HP1, Eff/UbcD1, ATM, the components of the Mre11-Rad50-Nbs (MRN complex, and the Woc transcription factor. These proteins do not share the terminin properties; they are evolutionarily conserved non-fast-evolving proteins that do not accumulate only telomeres and do not serve telomere-specific functions. We propose that following telomerase loss, Drosophila rapidly evolved terminin to bind chromosome ends in a sequence-independent manner. This hypothesis suggests that terminin is the functional analog of the shelterin complex that protects human telomeres. The non-terminin proteins are instead likely to correspond to ancestral telomere-associated proteins that did not evolve as rapidly as terminin because of the functional constraints imposed by their involvement in diverse cellular processes. Thus, it appears that the main difference between Drosophila and human telomeres is in the protective complexes that specifically associate with the DNA termini. We believe that Drosophila telomeres offer excellent opportunities for investigations on human telomere biology. The identification of additional Drosophila genes encoding non-terminin proteins involved in telomere protection might lead to the discovery of novel components of human telomeres.

  9. USGS analysis of the Australian UNCLOS submission

    Science.gov (United States)

    Hutchinson, Deborah R.; Rowland, Robert W.

    2006-01-01

    In November 2004, the Government of Australia made a submission to the Commission on the Limits of the Continental Shelf (CLCS) for 10 extended continental shelf (ECS) regions, utilizing Article-76 of the United Nations Convention on the Law of the Sea (UNCLOS). With information provided in the Australian Executive Summary, the USGS examined the 10 regions of the submission from geological, morphological, and resource perspectives. By their own request, the Australians asked that CLCS take no action on the Australian-Antarctic Territory. The major limitation in this analysis is that no bathymetric soundings or detailed hydrographic profiles were provided in the Australian Executive Summary that might show why the Foot of the Slope (FOS) was chosen or where the 2,500-m contour is located. This represents a major limitation because more than half of the 4,205 boundary points utilize the bathymetric formula line and more than one-third of them utilize the bathymetric constraint line. CLCS decisions on the components of this submission may set a precedent for how ECSs are treated in future submissions. Some of the key decisions will cover (a) how a 'natural prolongation' of a continental margin is determined, particularly if a bathymetric saddle that appears to determine the prolongation is in deep water and is well outside of the 200-nm limit (Exmouth Plateau), (b) defining to what extent that plateaus, rises, caps, banks and spurs that are formed of oceanic crust and from oceanic processes can be considered to be 'natural prolongations' (Kerguelen Plateau), (c) to what degree UNCLOS recognizes reefs and uninhabited micro-islands (specifically, rocks and/or sand shoals) as islands that can have an EEZ (Middleton and Elizabeth Reefs north of Lord Howe Island), and (d) how the Foot of the Slope (FOS) is chosen (Great Australian Bight). The submission contains situations that are relevant to potential future U.S. submissions and are potentially analogous to certain

  10. Mutants dissecting development and behaviour in drosophila

    International Nuclear Information System (INIS)

    We have traced in this paper the progress in Drosophila genetics research from the 1960s, at the IARI, spearheaded by the visionary insight of M. S. Swaminathan. The work started with the study of indirect effect of radiation and the synergistic interaction of physical and chemical mutagens on chromosomal and genetic changes. This paved the way for the study of single gene mutants in dissecting developmental and behavioural processes. New genes discovered by us have been shown to encode conserved cell signalling molecules controlling developmental and behavioural pathways. With the complete sequencing of the Drosophila genome, in the year 2000, mounting evidence for the homology between Drosophila and human genes controlling genetic disorders became available. This has led to the fly becoming an indispensable tool for studying human diseases as well as a model to test for drugs and pharmaceuticals against human diseases and complex behavioural processes. For example wingless in Drosophila belongs to the conserved Wnt gene family and aberrant WNT signalling is linked to a range of human diseases, most notably cancer. Inhibition as well as activation of WNT signalling form the basis of an effective therapy for some cancers as well as several other clinical conditions. Recent experiments have shown that WNTs might also normally participate in self-renewal, proliferation or differentiation of stem cells and altering WNT signalling might be beneficial to the use of stem cells for therapeutic means. Likewise, the stambhA mutant of Drosophila which was discovered for its temperature-dependent paralytic behaviour is the fly homologue of Phospholipase Cβ. Phospholipase C mediated G protein signalling plays a central role in vital processes controlling epilepsy, vision, taste, and olfaction in animals. Proteins of the G-signalling pathway are of intense research interest since many human diseases involve defects in G-protein signalling pathways. In fact, approximately 50

  11. Nature Study, Aborigines and the Australian Kindergarten: Lessons from Martha Simpson's "Australian Programme Based on the Life and Customs of the Australian Black"

    Science.gov (United States)

    Jones, Jennifer

    2014-01-01

    This article examines an experimental kindergarten programme "Work in the Kindergarten: An Australian Programme based on the Life and Customs of the Australian Black" developed by Martha Simpson in early twentieth-century Australia. Here Simpson adapted international Revisionist Froebelian approaches to cultural epoch theory and nature…

  12. Decolonising Australian Psychology: Discourses, Strategies, and Practice

    Directory of Open Access Journals (Sweden)

    Pat Dudgeon

    2015-08-01

    Full Text Available Colonisation in Australia has had a devastating and lasting impact on the wellbeing of Aboriginal and Torres Strait Islander peoples in Australia (herein referred to as Indigenous Australians. This paper discusses the role of psychology in Australia and the negative impact that certain disciplinary theories and practices have had on Indigenous Australians. The impact has been further exacerbated by the failure of mainstream policy makers and mental health practitioners to recognise the key, distinctive cultural and social determinants that contribute to Aboriginal health and wellbeing. There is a growing response by Aboriginal psychologists, critical social theorists, and their allies to decolonise psychological theory and practice to redress this situation. This paper outlines key decolonising strategies that have been effective in interrupting those aspects of psychology that are inimical to Aboriginal wellbeing.

  13. The Portrayal of Indigenous Health in Selected Australian Media

    Directory of Open Access Journals (Sweden)

    Melissa J. Stoneham

    2014-04-01

    Full Text Available It is acknowledged that health outcomes for Australian Indigenous peoples are lower than those of non-Indigenous Australians. Research suggests negative media in relation to Indigenous Australians perpetuates racist stereotypes among the wider population and impacts on the health of Indigenous Australians. This study examined the media portrayal of Indigenous Australian public health issues in selected media over a twelve month period and found that, overwhelmingly, the articles were negative in their portrayal of Indigenous health. A total of 74 percent of the coverage of Australian Indigenous related articles were negative, 15 percent were positive, and 11 percent were neutral. The most common negative subject descriptors related to alcohol, child abuse, petrol sniffing, violence, suicide, deaths in custody, and crime.

  14. Inventories in the Australian business cycle

    OpenAIRE

    Chindamo, Phillip

    2010-01-01

    This Economics Research Note examines inventories in the business cycle for Australia covering the period since the mid 1980s. The Australian Bureau of Statistics (ABS) defines inventories as all materials etc., work in progress and finished goods owned by a business, whether held at locations of the business or elsewhere. These items are usually held by businesses in anticipation of a product’s sale. Inventory investment is counted as an additional contribution to gross domestic product (...

  15. "Bridging the Gap" through Australian Cultural Astronomy

    OpenAIRE

    Hamacher, Duane W.; Norris, Ray P.

    2011-01-01

    For more than 50,000 years, Indigenous Australians have incorporated celestial events into their oral traditions and used the motions of celestial bodies for navigation, time-keeping, food economics, and social structure. In this paper, we explore the ways in which Aboriginal people made careful observations of the sky, measurements of celestial bodies, and incorporated astronomical events into complex oral traditions by searching for written records of time-keeping using celestial bodies, th...

  16. Assessing the Research Performance of Australian Universities

    OpenAIRE

    Valadkhani, Abbas; Worthington, Andrew

    2006-01-01

    This paper identifies new classifications of Australian universities based on their total and per-academic staff research outputs using the data for the period 1998-2002. We define research performance in terms of audited numbers of PhD completions, publications and grants (in accordance with rules established by the Department of Education, Science and Training). Our analysis indicates that (a) the highest achievers consists of the seven Group of Eight (Go8) universities; (b) the top-three...

  17. Representative Democracy in Australian Local Government

    OpenAIRE

    Colin Hearfield; Brian Dollery

    2009-01-01

    In an assessment of representative democracy in Australian local government, this paper considers long-run changes in forms of political representation, methods of vote counting, franchise arrangements, numbers of local government bodies and elected representatives, as well as the thorny question of constitutional recognition. This discussion is set against the background of ongoing tensions between the drive for economic efficiency and the maintenance of political legitimacy, along with more...

  18. The Australian Credit Default Swap Markets

    OpenAIRE

    Daniel Fabbro

    2011-01-01

    The Australian credit default swap (CDS) market has been increasingly used by financial institutions to trade and manage credit risk. As a result, there has been greater use of the market as a source of credit risk pricing information. Similarities between CDS and bonds allow pricing in the two markets to be compared. However, the CDS market has a greater tendency at times to be affected by poor liquidity, which complicates the interpretation of CDS pricing, particularly when there are large ...

  19. New Measures of Australian Corporate Credit Spreads

    OpenAIRE

    Ivailo Arsov; Matthew Brooks; Mitch Kosev

    2013-01-01

    Australian corporations access bond markets both domestically and offshore. Despite this, there is a lack of publicly available data on bond market conditions faced by non-financial corporations (NFCs). This gap in the data is particularly apparent at longer maturities where the low level of bond issuance, especially in the domestic market, makes it difficult to gauge the long-term credit spreads faced by resident issuers. To address this lack of data, the article presents a method for estima...

  20. Ownership Structure and Corporate Performance: Australian Evidence

    OpenAIRE

    Julian Fishman; Gerard Gannon; Russell Vinning

    2007-01-01

    This paper seeks to analyse the relationship between ownership structure and corporate performance for fifty firms listed on the Australian Stock Exchange during 2002-2003. The study initially tests a two equation model similar to that in the existing literature, but is distinguished from prior literature by subsequently reclassifying leverage. By categorising leverage as an endogenous variable, an examination of the relationship between ownership and performance is undertaken through ordinar...

  1. Australian Eager to Enter China's LNG Market

    Institute of Scientific and Technical Information of China (English)

    Li Han

    2002-01-01

    @@ Australian Trade Minister Mark Vaile made a trip to China, Japan and Republic of Korea in mid-April in an attempt to locate potential users for LNG from the northwestern continental shelf of the country.Australia has made tenacious efforts for the annual 3-million-ton LNG supply project in China's Guangdong Province. In addition, Australia also hopes to have more users in Japan and Republic of Korea.

  2. Plasticity in the Drosophila larval visual System

    Directory of Open Access Journals (Sweden)

    Abud J Farca-Luna

    2013-07-01

    Full Text Available The remarkable ability of the nervous system to modify its structure and function is mostly experience and activity modulated. The molecular basis of neuronal plasticity has been studied in higher behavioral processes, such as learning and memory formation. However, neuronal plasticity is not restricted to higher brain functions, but may provide a basic feature of adaptation of all neural circuits. The fruit fly Drosophila melanogaster provides a powerful genetic model to gain insight into the molecular basis of nervous system development and function. The nervous system of the larvae is again a magnitude simpler than its adult counter part, allowing the genetic assessment of a number of individual genetically identifiable neurons. We review here recent progress on the genetic basis of neuronal plasticity in developing and functioning neural circuits focusing on the simple visual system of the Drosophila larva.

  3. Remembering components of food in Drosophila

    Directory of Open Access Journals (Sweden)

    Gaurav eDas

    2016-02-01

    Full Text Available Remembering features of past feeding experience can refine foraging and food choice. Insects can learn to associate sensory cues with components of food, such as sugars, amino acids, water, salt, alcohol, toxins and pathogens. In the fruit fly Drosophila some food components activate unique subsets of dopaminergic neurons that innervate distinct functional zones on the mushroom bodies. This architecture suggests that the overall dopaminergic neuron population could provide a potential cellular substrate through which the fly might learn to value a variety of food components. In addition, such an arrangement predicts that individual component memories reside in unique locations. Dopaminergic neurons are also critical for food memory consolidation and deprivation-state dependent motivational control of the expression of food-relevant memories. Here we review our current knowledge of how nutrient-specific memories are formed, consolidated and specifically retrieved in insects, with a particular emphasis on Drosophila.

  4. [The comeback of mitochondria in Drosophila apoptosis].

    Science.gov (United States)

    Clavier, Amandine; Rincheval-Arnold, Aurore; Mignotte, Bernard; Guénal, Isabelle

    2016-05-01

    The role of the mitochondrion in mammalian cell apoptosis has been established since the mid-1990s. However, the importance of this organelle in non-mammalian apoptosis has long been regarded as minor, notably because of the absence of a crucial role for cytochrome c in caspase activation. Recent results indicate that the control of caspase activation and apoptosis in Drosophila cell death occurs at the mitochondrial level. Numerous proteins that appear key for Drosophila apoptosis regulation constitutively or transiently bind to mitochondria. They participate in the cell death process at different levels such as degradation of an IAP caspase inhibitor, production of mitochondrial reactive oxygen species or stimulation of the mitochondrial fission machinery. The aim of this review is to take stock of these events that might have their counterpart in humans. PMID:27225920

  5. Motor Control of Drosophila Courtship Song

    Directory of Open Access Journals (Sweden)

    Troy R. Shirangi

    2013-11-01

    Full Text Available Many animals utilize acoustic signals—or songs—to attract mates. During courtship, Drosophila melanogaster males vibrate a wing to produce trains of pulses and extended tone, called pulse and sine song, respectively. Courtship songs in the genus Drosophila are exceedingly diverse, and different song features appear to have evolved independently of each other. How the nervous system allows such diversity to evolve is not understood. Here, we identify a wing muscle in D. melanogaster (hg1 that is uniquely male-enlarged. The hg1 motoneuron and the sexually dimorphic development of the hg1 muscle are required specifically for the sine component of the male song. In contrast, the motoneuron innervating a sexually monomorphic wing muscle, ps1, is required specifically for a feature of pulse song. Thus, individual wing motor pathways can control separate aspects of courtship song and may provide a “modular” anatomical substrate for the evolution of diverse songs.

  6. Exquisite Light Sensitivity of Drosophila melanogaster Cryptochrome

    Science.gov (United States)

    Vinayak, Pooja; Coupar, Jamie; Hughes, S. Emile; Fozdar, Preeya; Kilby, Jack; Garren, Emma; Yoshii, Taishi; Hirsh, Jay

    2013-01-01

    Drosophila melanogaster shows exquisite light sensitivity for modulation of circadian functions in vivo, yet the activities of the Drosophila circadian photopigment cryptochrome (CRY) have only been observed at high light levels. We studied intensity/duration parameters for light pulse induced circadian phase shifts under dim light conditions in vivo. Flies show far greater light sensitivity than previously appreciated, and show a surprising sensitivity increase with pulse duration, implying a process of photic integration active up to at least 6 hours. The CRY target timeless (TIM) shows dim light dependent degradation in circadian pacemaker neurons that parallels phase shift amplitude, indicating that integration occurs at this step, with the strongest effect in a single identified pacemaker neuron. Our findings indicate that CRY compensates for limited light sensitivity in vivo by photon integration over extraordinarily long times, and point to select circadian pacemaker neurons as having important roles. PMID:23874218

  7. Exquisite light sensitivity of Drosophila melanogaster cryptochrome.

    Directory of Open Access Journals (Sweden)

    Pooja Vinayak

    Full Text Available Drosophila melanogaster shows exquisite light sensitivity for modulation of circadian functions in vivo, yet the activities of the Drosophila circadian photopigment cryptochrome (CRY have only been observed at high light levels. We studied intensity/duration parameters for light pulse induced circadian phase shifts under dim light conditions in vivo. Flies show far greater light sensitivity than previously appreciated, and show a surprising sensitivity increase with pulse duration, implying a process of photic integration active up to at least 6 hours. The CRY target timeless (TIM shows dim light dependent degradation in circadian pacemaker neurons that parallels phase shift amplitude, indicating that integration occurs at this step, with the strongest effect in a single identified pacemaker neuron. Our findings indicate that CRY compensates for limited light sensitivity in vivo by photon integration over extraordinarily long times, and point to select circadian pacemaker neurons as having important roles.

  8. Imaging Calcium in Drosophila at Egg Activation.

    Science.gov (United States)

    Derrick, Christopher J; York-Andersen, Anna H; Weil, Timothy T

    2016-01-01

    Egg activation is a universal process that includes a series of events to allow the fertilized egg to complete meiosis and initiate embryonic development. One aspect of egg activation, conserved across all organisms examined, is a change in the intracellular concentration of calcium (Ca(2+)) often termed a 'Ca(2+) wave'. While the speed and number of oscillations of the Ca(2+) wave varies between species, the change in intracellular Ca(2+) is key in bringing about essential events for embryonic development. These changes include resumption of the cell cycle, mRNA regulation, cortical granule exocytosis, and rearrangement of the cytoskeleton. In the mature Drosophila egg, activation occurs in the female oviduct prior to fertilization, initiating a series of Ca(2+)-dependent events. Here we present a protocol for imaging the Ca(2+) wave in Drosophila. This approach provides a manipulable model system to interrogate the mechanism of the Ca(2+) wave and the downstream changes associated with it. PMID:27584955

  9. The Australian Centre for Minesite Rehabilitation Research

    International Nuclear Information System (INIS)

    The Australian Centre for Minesite Rehabilitation Research (ACMRR) is a joint venture between the Australian mining industry through the Australian Mineral Industries Research Association Ltd. (AMIRA) and three of the organizations working most actively in this area in Australia: CSIRO Minesite Rehabilitation Research Program; University of Queensland Centre for Mined Land Rehabilitation; and Curtin University Mulga Research Centre. The ACMRR was established in July 1993 to provide a national framework to conduct Strategic Research into minesite rehabilitation. It is an industry led and funded initiative. The Goals of the Centre include: to conduct strategic research into minesite rehabilitation to provide sustainable environmental solutions which are acceptable to industry, government and the community; to be recognized as a center of excellence undertaking commissioned research on minesite rehabilitation in an independent and thorough manner; to provide scientific and technological foundations to facilitate industry and government in setting acceptable standards; to act as networking and communications focus; and to enhance education and training in minesite rehabilitation. Strategic Research Programs in: Water Systems--downstream surface and groundwater quality; Land--the long-term behavior and stability of constructed landforms; Ecosystems--the long-term sustainability of constructed landforms; Waste--the long-term treatment and disposal of waste products; will allow the ACMRR to achieve these goals through specific research projects in these areas, developed with industry sponsors. This paper will discuss their progress to date, research projects underway, and plans for the future

  10. Deep Roots for Aboriginal Australian Y Chromosomes.

    Science.gov (United States)

    Bergström, Anders; Nagle, Nano; Chen, Yuan; McCarthy, Shane; Pollard, Martin O; Ayub, Qasim; Wilcox, Stephen; Wilcox, Leah; van Oorschot, Roland A H; McAllister, Peter; Williams, Lesley; Xue, Yali; Mitchell, R John; Tyler-Smith, Chris

    2016-03-21

    Australia was one of the earliest regions outside Africa to be colonized by fully modern humans, with archaeological evidence for human presence by 47,000 years ago (47 kya) widely accepted [1, 2]. However, the extent of subsequent human entry before the European colonial age is less clear. The dingo reached Australia about 4 kya, indirectly implying human contact, which some have linked to changes in language and stone tool technology to suggest substantial cultural changes at the same time [3]. Genetic data of two kinds have been proposed to support gene flow from the Indian subcontinent to Australia at this time, as well: first, signs of South Asian admixture in Aboriginal Australian genomes have been reported on the basis of genome-wide SNP data [4]; and second, a Y chromosome lineage designated haplogroup C(∗), present in both India and Australia, was estimated to have a most recent common ancestor around 5 kya and to have entered Australia from India [5]. Here, we sequence 13 Aboriginal Australian Y chromosomes to re-investigate their divergence times from Y chromosomes in other continents, including a comparison of Aboriginal Australian and South Asian haplogroup C chromosomes. We find divergence times dating back to ∼50 kya, thus excluding the Y chromosome as providing evidence for recent gene flow from India into Australia. PMID:26923783

  11. Calcium Intake in Elderly Australian Women Is Inadequate

    OpenAIRE

    Colin W. Binns; Xingqiong Meng; Kerr, Deborah A; Kun Zhu; Amanda Devine; Vicky Solah; Richard L. Prince

    2010-01-01

    The role of calcium in the prevention of bone loss in later life has been well established but little data exist on the adequacy of calcium intakes in elderly Australian women. The aim of this study was to compare the dietary intake including calcium of elderly Australian women with the Australian dietary recommendation, and to investigate the prevalence of calcium supplement use in this population. Community-dwelling women aged 70–80 years were randomly recruited using the Electoral Roll for...

  12. How Australian and Indonesian Universities Treat Plagiarism: A Comparative Study

    OpenAIRE

    Bambang Yudi Cahyono

    2016-01-01

    This article is a part of a larger study comparing various aspects of policies on plagiarism in two university contexts. It compares policies on plagiarism in universities in Australia and Indonesia. The results of this comparative study showed that Australian and Indonesian universities treat plagiarism differently. Australian universities treat plagiarism explicitly in their university policies. In Australian universities, plagiarism is defined clearly and forms of plagiarism are explained ...

  13. Recombineering Homologous Recombination Constructs in Drosophila

    OpenAIRE

    Carreira-Rosario, Arnaldo; Scoggin, Shane; Shalaby, Nevine A.; Williams, Nathan David; Hiesinger, P. Robin; Buszczak, Michael

    2013-01-01

    The continued development of techniques for fast, large-scale manipulation of endogenous gene loci will broaden the use of Drosophila melanogaster as a genetic model organism for human-disease related research. Recent years have seen technical advancements like homologous recombination and recombineering. However, generating unequivocal null mutations or tagging endogenous proteins remains a substantial effort for most genes. Here, we describe and demonstrate techniques for using recombineeri...

  14. Three-dimensional imaging of Drosophila melanogaster.

    Directory of Open Access Journals (Sweden)

    Leeanne McGurk

    Full Text Available BACKGROUND: The major hindrance to imaging the intact adult Drosophila is that the dark exoskeleton makes it impossible to image through the cuticle. We have overcome this obstacle and describe a method whereby the internal organs of adult Drosophila can be imaged in 3D by bleaching and clearing the adult and then imaging using a technique called optical projection tomography (OPT. The data is displayed as 2D optical sections and also in 3D to provide detail on the shape and structure of the adult anatomy. METHODOLOGY: We have used OPT to visualize in 2D and 3D the detailed internal anatomy of the intact adult Drosophila. In addition this clearing method used for OPT was tested for imaging with confocal microscopy. Using OPT we have visualized the size and shape of neurodegenerative vacuoles from within the head capsule of flies that suffer from age-related neurodegeneration due to a lack of ADAR mediated RNA-editing. In addition we have visualized tau-lacZ expression in 2D and 3D. This shows that the wholemount adult can be stained without any manipulation and that this stain penetrates well as we have mapped the localization pattern with respect to the internal anatomy. CONCLUSION: We show for the first time that the intact adult Drosophila can be imaged in 3D using OPT, also we show that this method of clearing is also suitable for confocal microscopy to image the brain from within the intact head. The major advantage of this is that organs can be represented in 3D in their natural surroundings. Furthermore optical sections are generated in each of the three planes and are not prone to the technical limitations that are associated with manual sectioning. OPT can be used to dissect mutant phenotypes and to globally map gene expression in both 2D and 3D.

  15. Structure and Development of Glia in Drosophila

    OpenAIRE

    Hartenstein, Volker

    2011-01-01

    Insect glia represents a conspicuous and diverse population of cells and plays a role in controlling neuronal progenitor proliferation, axonal growth, neuronal differentiation and maintenance, and neuronal function. Genetic studies in Drosophila have elucidated many aspects of glial structure, function and development. Just as in vertebrates, it appears as if different classes of glial cells are specialized for different functions. Based on topology and cell shape, glial cells of the central ...

  16. The development of the Drosophila larval brain.

    Science.gov (United States)

    Hartenstein, Volker; Spindler, Shana; Pereanu, Wayne; Fung, Siaumin

    2008-01-01

    In this chapter we will start out by describing in more detail the progenitors of the nervous system, the neuroblasts and ganglion mother cells. Subsequently we will survey the generic cell types that make up the developing Drosophila brain, namely neurons, glial cells and tracheal cells. Finally, we will attempt a synopsis of the neuronal connectivity of the larval brain that can be deduced from the analysis of neural lineages and their relationship to neuropile compartments. PMID:18683635

  17. A Drosophila melanogaster model of classic galactosemia

    OpenAIRE

    Kushner, Rebekah F.; Ryan, Emily L.; Sefton, Jennifer M. I.; Rebecca D Sanders; Lucioni, Patricia Jumbo; Kenneth H Moberg; Fridovich-Keil, Judith L.

    2010-01-01

    Classic galactosemia is a potentially lethal disorder that results from profound impairment of galactose-1-phosphate uridylyltransferase (GALT). Despite decades of research, the underlying pathophysiology of classic galactosemia remains unclear, in part owing to the lack of an appropriate animal model. Here, we report the establishment of a Drosophila melanogaster model of classic galactosemia; this is the first whole-animal genetic model to mimic aspects of the patient phenotype. Analogous t...

  18. Quantification of Food Intake in Drosophila

    OpenAIRE

    Richard Wong; Matthew D W Piper; Bregje Wertheim; Linda Partridge

    2009-01-01

    Measurement of food intake in the fruit fly Drosophila melanogaster is often necessary for studies of behaviour, nutrition and drug administration. There is no reliable and agreed method for measuring food intake of flies in undisturbed, steady state, and normal culture conditions. We report such a method, based on measurement of feeding frequency by proboscis-extension, validated by short-term measurements of food dye intake. We used the method to demonstrate that (a) female flies feed more ...

  19. Tools for neuroanatomy and neurogenetics in Drosophila

    OpenAIRE

    Pfeiffer, Barret D.; Jenett, Arnim; Hammonds, Ann S.; Ngo, Teri-T B.; Misra, Sima; Murphy, Christine; Scully, Audra; Carlson, Joseph W.; Wan, Kenneth H.; Laverty, Todd R.; Mungall, Chris; Svirskas, Rob; Kadonaga, James T.; Doe, Chris Q.; Eisen, Michael B

    2008-01-01

    We demonstrate the feasibility of generating thousands of transgenic Drosophila melanogaster lines in which the expression of an exogenous gene is reproducibly directed to distinct small subsets of cells in the adult brain. We expect the expression patterns produced by the collection of 5,000 lines that we are currently generating to encompass all neurons in the brain in a variety of intersecting patterns. Overlapping 3-kb DNA fragments from the flanking noncoding and intronic regions of gene...

  20. Functional neuroanatomy of Drosophila olfactory memory formation

    OpenAIRE

    Guven-Ozkan, Tugba; Davis, Ronald L.

    2014-01-01

    New approaches, techniques and tools invented over the last decade and a half have revolutionized the functional dissection of neural circuitry underlying Drosophila learning. The new methodologies have been used aggressively by researchers attempting to answer three critical questions about olfactory memories formed with appetitive and aversive reinforcers: (1) Which neurons within the olfactory nervous system mediate the acquisition of memory? (2) What is the complete neural circuitry exten...

  1. Detection of Cell Death in Drosophila Tissues

    Science.gov (United States)

    Vasudevan, Deepika; Ryoo, Hyung Don

    2016-01-01

    Drosophila has served as a particularly attractive model to study cell death due to the vast array of tools for genetic manipulation under defined spatial and temporal conditions in vivo as well as in cultured cells. These genetic methods have been well supplemented by enzymatic assays and a panel of antibodies recognizing cell death markers. This chapter discusses reporters, mutants and assays used by various laboratories to study cell death in the context of development and in response to external insults. PMID:27108437

  2. Visualizing the spindle checkpoint in Drosophila spermatocytes

    OpenAIRE

    Rebollo, Elena; González, Cayetano

    2000-01-01

    The spindle assembly checkpoint detects defects in spindle structure or in the alignment of the chromosomes on the metaphase plate and delays the onset of anaphase until defects are corrected. Thus far, the evidence regarding the presence of a spindle checkpoint during meiosis in male Drosophila has been indirect and contradictory. On the one hand, chromosomes without pairing partners do not prevent meiosis progression. On the other hand, some conserved components of the spindle checkpoint ma...

  3. A Taste of the Drosophila Gustatory Receptors

    OpenAIRE

    Montell, Craig

    2009-01-01

    Insects such as the fruit fly, Drosophila melanogaster, rely on contact chemosensation to detect nutrient-rich foods, to avoid consuming toxic chemicals, and to select mates and hospitable zones to deposit eggs. Flies sense tastants and non-volatile pheromones through gustatory bristles and pegs distributed on multiple body parts including the proboscis, wing margins, legs and ovipositor. The sensilla house gustatory receptor neurons, which express members of the family of 68 gustatory recept...

  4. Visualizing the spindle checkpoint in Drosophila spermatocytes

    Science.gov (United States)

    Rebollo, Elena; González, Cayetano

    2000-01-01

    The spindle assembly checkpoint detects defects in spindle structure or in the alignment of the chromosomes on the metaphase plate and delays the onset of anaphase until defects are corrected. Thus far, the evidence regarding the presence of a spindle checkpoint during meiosis in male Drosophila has been indirect and contradictory. On the one hand, chromosomes without pairing partners do not prevent meiosis progression. On the other hand, some conserved components of the spindle checkpoint machinery are expressed in these cells and behave as their homologue proteins do in systems with an active spindle checkpoint. To establish whether the spindle checkpoint is active in Drosophila spermatocytes we have followed meiosis progression by time-lapse microscopy under conditions where the checkpoint is likely to be activated. We have found that the presence of a relatively high number of misaligned chromosomes or a severe disruption of the meiotic spindle results in a significant delay in the time of entry into anaphase. These observations provide the first direct evidence substantiating the activity of a meiotic spindle checkpoint in male Drosophila. PMID:11256627

  5. Global patterns of sequence evolution in Drosophila

    Directory of Open Access Journals (Sweden)

    Marín Ignacio

    2007-11-01

    Full Text Available Abstract Background Sequencing of the genomes of several Drosophila allows for the first precise analyses of how global sequence patterns change among multiple, closely related animal species. A basic question is whether there are characteristic features that differentiate chromosomes within a species or between different species. Results We explored the euchromatin of the chromosomes of seven Drosophila species to establish their global patterns of DNA sequence diversity. Between species, differences in the types and amounts of simple sequence repeats were found. Within each species, the autosomes have almost identical oligonucleotide profiles. However, X chromosomes and autosomes have, in all species, a qualitatively different composition. The X chromosomes are less complex than the autosomes, containing both a higher amount of simple DNA sequences and, in several cases, chromosome-specific repetitive sequences. Moreover, we show that the right arm of the X chromosome of Drosophila pseudoobscura, which evolved from an autosome 10 – 18 millions of years ago, has a composition which is identical to that of the original, left arm of the X chromosome. Conclusion The consistent differences among species, differences among X chromosomes and autosomes and the convergent evolution of X and neo-X chromosomes demonstrate that strong forces are acting on drosophilid genomes to generate peculiar chromosomal landscapes. We discuss the relationships of the patterns observed with differential recombination and mutation rates and with the process of dosage compensation.

  6. ‘Peer pressure’ in larval Drosophila?

    Directory of Open Access Journals (Sweden)

    Thomas Niewalda

    2014-06-01

    Full Text Available Understanding social behaviour requires a study case that is simple enough to be tractable, yet complex enough to remain interesting. Do larval Drosophila meet these requirements? In a broad sense, this question can refer to effects of the mere presence of other larvae on the behaviour of a target individual. Here we focused in a more strict sense on ‘peer pressure’, that is on the question of whether the behaviour of a target individual larva is affected by what a surrounding group of larvae is doing. We found that innate olfactory preference of a target individual was neither affected (i by the level of innate olfactory preference in the surrounding group nor (ii by the expression of learned olfactory preference in the group. Likewise, learned olfactory preference of a target individual was neither affected (iii by the level of innate olfactory preference of the surrounding group nor (iv by the learned olfactory preference the group was expressing. We conclude that larval Drosophila thus do not take note of specifically what surrounding larvae are doing. This implies that in a strict sense, and to the extent tested, there is no social interaction between larvae. These results validate widely used en mass approaches to the behaviour of larval Drosophila.

  7. Pervasive natural selection in the Drosophila genome?

    Directory of Open Access Journals (Sweden)

    Guy Sella

    2009-06-01

    Full Text Available Over the past four decades, the predominant view of molecular evolution saw little connection between natural selection and genome evolution, assuming that the functionally constrained fraction of the genome is relatively small and that adaptation is sufficiently infrequent to play little role in shaping patterns of variation within and even between species. Recent evidence from Drosophila, reviewed here, suggests that this view may be invalid. Analyses of genetic variation within and between species reveal that much of the Drosophila genome is under purifying selection, and thus of functional importance, and that a large fraction of coding and noncoding differences between species are adaptive. The findings further indicate that, in Drosophila, adaptations may be both common and strong enough that the fate of neutral mutations depends on their chance linkage to adaptive mutations as much as on the vagaries of genetic drift. The emerging evidence has implications for a wide variety of fields, from conservation genetics to bioinformatics, and presents challenges to modelers and experimentalists alike.

  8. dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis

    Science.gov (United States)

    Aerts, Stein; Casares, Fernando; Janody, Florence

    2016-01-01

    Proper organ patterning depends on a tight coordination between cell proliferation and differentiation. The patterning of Drosophila retina occurs both very fast and with high precision. This process is driven by the dynamic changes in signaling activity of the conserved Hedgehog (Hh) pathway, which coordinates cell fate determination, cell cycle and tissue morphogenesis. Here we show that during Drosophila retinogenesis, the retinal determination gene dachshund (dac) is not only a target of the Hh signaling pathway, but is also a modulator of its activity. Using developmental genetics techniques, we demonstrate that dac enhances Hh signaling by promoting the accumulation of the Gli transcription factor Cubitus interruptus (Ci) parallel to or downstream of fused. In the absence of dac, all Hh-mediated events associated to the morphogenetic furrow are delayed. One of the consequences is that, posterior to the furrow, dac- cells cannot activate a Roadkill-Cullin3 negative feedback loop that attenuates Hh signaling and which is necessary for retinal cells to continue normal differentiation. Therefore, dac is part of an essential positive feedback loop in the Hh pathway, guaranteeing the speed and the accuracy of Drosophila retinogenesis. PMID:27442438

  9. Sporting Chance: Indigenous Participation in Australian Sport History

    Directory of Open Access Journals (Sweden)

    Sean Gorman

    2010-08-01

    Full Text Available For many non-Indigenous Australians the only time they have any engagement with Indigenous peoples, history or issues is through watching sport on television or being at a football match at the MCG. This general myopia and indifference by settler Australians with Indigenous Australians manifests itself in many ways but perhaps most obscenely in the simple fact that Indigenous Australians die nearly 20 years younger than the rest of Australias citizens. Many non-Indigenous Australians do not know this. Sport in many ways has offered Indigenous Australians a platform from which to begin the slow, hard process for social justice and equity to be actualised. This paper will discuss the participation of Indigenous Australians in sport and show how sport has enabled Indigenous Australians to create a space so that they can speak out against the injustices they have experienced and to further improve on relations going into the future. The central contention is that through sport all Australians can begin a process of engaging with Indigenous history as a means to improve race relations between the two groups.

  10. Persistence of a Wolbachia infection frequency cline in Drosophila melanogaster and the possible role of reproductive dormancy.

    Science.gov (United States)

    Kriesner, Peter; Conner, William R; Weeks, Andrew R; Turelli, Michael; Hoffmann, Ary A

    2016-05-01

    Field populations of arthropods are often polymorphic for Wolbachia but the factors maintaining intermediate Wolbachia frequencies are generally not understood. In Drosophila melanogaster, Wolbachia frequencies are highly variable across the globe. We document the persistence of a Wolbachia infection frequency cline in D. melanogaster populations from eastern Australia across at least 20 years, with frequencies generally high in the tropics but lower in cool temperate regions. The results are interpreted using a model of frequency dynamics incorporating cytoplasmic incompatibility (CI), imperfect maternal transmission and Wolbachia effects on fitness. Clinal variation is less pronounced in eastern North America which may reflect annual recolonization at higher latitudes. Limited samples from Africa from latitudes matching our tropical and subtropical samples from Australia and North America show comparably high infection frequencies, but some equatorial samples show lower frequencies. Adult dormancy across cold periods may contribute to the Australian Wolbachia cline. Infected flies exposed to cold conditions for an extended period had reduced fecundity and viability, an effect not evident in unexposed controls. These fitness costs may contribute to the relatively low Wolbachia frequencies in Australian temperate areas; whereas different processes, including CI induced by young males, may contribute to higher frequencies in tropical locations. PMID:27076356

  11. Ionizing radiation causes the stress response in Drosophila melanogaster

    International Nuclear Information System (INIS)

    Potentiality of the stress-reaction arising in Drosophila melanogaster under gamma-irradiation of the source with 137Cs (irradiation dose is 10 Gy , radiation dose rate amounts 180 c Gy/min) is studied. It is shown that radiation induces the stress-reaction in Drosophila resulting in alterations in energetic metabolism (biogenic amines metabolic system) and in reproductive function

  12. The developmental transcriptome of Drosophila melanogaster

    Energy Technology Data Exchange (ETDEWEB)

    University of Connecticut; Graveley, Brenton R.; Brooks, Angela N.; Carlson, Joseph W.; Duff, Michael O.; Landolin, Jane M.; Yang, Li; Artieri, Carlo G.; van Baren, Marijke J.; Boley, Nathan; Booth, Benjamin W.; Brown, James B.; Cherbas, Lucy; Davis, Carrie A.; Dobin, Alex; Li, Renhua; Lin, Wei; Malone, John H.; Mattiuzzo, Nicolas R.; Miller, David; Sturgill, David; Tuch, Brian B.; Zaleski, Chris; Zhang, Dayu; Blanchette, Marco; Dudoit, Sandrine; Eads, Brian; Green, Richard E.; Hammonds, Ann; Jiang, Lichun; Kapranov, Phil; Langton, Laura; Perrimon, Norbert; Sandler, Jeremy E.; Wan, Kenneth H.; Willingham, Aarron; Zhang, Yu; Zou, Yi; Andrews, Justen; Bicke, Peter J.; Brenner, Steven E.; Brent, Michael R.; Cherbas, Peter; Gingeras, Thomas R.; Hoskins, Roger A.; Kaufman, Thomas C.; Oliver, Brian; Celniker, Susan E.

    2010-12-02

    Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites. Full discovery and annotation are pre-requisites for understanding how the regulation of transcription, splicing and RNA editing directs the development of this complex organism. Here we used RNA-Seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct developmental stages. We identified 111,195 new elements, including thousands of genes, coding and non-coding transcripts, exons, splicing and editing events, and inferred protein isoforms that previously eluded discovery using established experimental, prediction and conservation-based approaches. These data substantially expand the number of known transcribed elements in the Drosophila genome and provide a high-resolution view of transcriptome dynamics throughout development. Drosophila melanogaster is an important non-mammalian model system that has had a critical role in basic biological discoveries, such as identifying chromosomes as the carriers of genetic information and uncovering the role of genes in development. Because it shares a substantial genic content with humans, Drosophila is increasingly used as a translational model for human development, homeostasis and disease. High-quality maps are needed for all functional genomic elements. Previous studies demonstrated that a rich collection of genes is deployed during the life cycle of the fly. Although expression profiling using microarrays has revealed the expression of, 13,000 annotated genes, it is difficult to map splice junctions and individual base modifications generated by RNA editing using such approaches. Single-base resolution is essential to define precisely the elements that comprise the Drosophila transcriptome. Estimates of the number of transcript isoforms are less accurate than estimates of the number of genes

  13. Intestinal stem cells in the adult Drosophila midgut

    International Nuclear Information System (INIS)

    Drosophila has long been an excellent model organism for studying stem cell biology. Notably, studies of Drosophila's germline stem cells have been instrumental in developing the stem cell niche concept. The recent discovery of somatic stem cells in adult Drosophila, particularly the intestinal stem cells (ISCs) of the midgut, has established Drosophila as an exciting model to study stem cell-mediated adult tissue homeostasis and regeneration. Here, we review the major signaling pathways that regulate the self-renewal, proliferation and differentiation of Drosophila ISCs, discussing how this regulation maintains midgut homeostasis and mediates regeneration of the intestinal epithelium after injury. -- Highlights: ► The homeostasis and regeneration of adult fly midguts are mediated by ISCs. ► Damaged enterocytes induce the proliferation of intestinal stem cells (ISC). ► EGFR and Jak/Stat signalings mediate compensatory ISC proliferation. ► Notch signaling regulates ISC self-renewal and differentiation.

  14. Rhodopsin 7–The unusual Rhodopsin in Drosophila

    Science.gov (United States)

    2016-01-01

    Rhodopsins are the major photopigments in the fruit fly Drosophila melanogaster. Drosophila express six well-characterized Rhodopsins (Rh1–Rh6) with distinct absorption maxima and expression pattern. In 2000, when the Drosophila genome was published, a novel Rhodopsin gene was discovered: Rhodopsin 7 (Rh7). Rh7 is highly conserved among the Drosophila genus and is also found in other arthropods. Phylogenetic trees based on protein sequences suggest that the seven Drosophila Rhodopsins cluster in three different groups. While Rh1, Rh2 and Rh6 form a “vertebrate-melanopsin-type”–cluster, and Rh3, Rh4 and Rh5 form an “insect-type”-Rhodopsin cluster, Rh7 seem to form its own cluster. Although Rh7 has nearly all important features of a functional Rhodopsin, it differs from other Rhodopsins in its genomic and structural properties, suggesting it might have an overall different role than other known Rhodopsins. PMID:27651995

  15. Isolation of protease-free alcohol dehydrogenase (ADH) from Drosophila simulans and several homozygous and heterozygous Drosophila melanogaster variants

    NARCIS (Netherlands)

    Smilda, T; Lamme, DA; Collu, G; Jekel, PA; Reinders, P; Beintema, JJ

    1998-01-01

    The enzyme alcohol dehydrogenase (ADH) from several naturally occurring ADH variants of Drosophila melanogaster and Drosophila simulans Lc,as isolated. Affinity chromatography with the ligand Cibacron Blue and elution with NAD(+) showed similar behavior for D. melanogaster ADH-FF, ADH-71k, and D. si

  16. Molecular cloning, functional expression, and gene silencing of two Drosophila receptors for the Drosophila neuropeptide pyrokinin-2

    DEFF Research Database (Denmark)

    Rosenkilde, Carina; Cazzamali, Giuseppe; Williamson, Michael;

    2003-01-01

    embryos and first instar larvae. In addition to the two Drosophila receptors, we also identified two probable pyrokinin receptors in the genomic database from the malaria mosquito Anopheles gambiae. The two Drosophila pyrokinin receptors are, to our knowledge, the first invertebrate pyrokinin receptors...

  17. Biological effects of radon in Drosophila; Efectos biologicos del radon en Drosophila

    Energy Technology Data Exchange (ETDEWEB)

    Pimentel P, A.E.; Tavera D, L.; Cruces M, M.P.; Arceo M, C.; Rosa D, M.E. de la

    1992-04-15

    The main objective of this investigation, is to study the biological effects of the Radon-222 at low dose in 'Drosophila melanogaster'. It is necessary to mention that these effects will analyze from the genetic point of view for: 1) To evaluate in which form the Radon-222 to low dose it influences in some genetic components of the adaptation in Drosophila, such as: fecundity, viability egg-adult and sex proportion. 2) To evaluate which is the genetic effect that induces the Radon to low dose by means of the SMART technique in Drosophila melanogaster, and this way to try of to identify which is the possible mechanism that causes the genetic damage to somatic level. The carried out investigation was divided in three stages: 1. Tests to the vacuum resistance. 2. Test of somatic mutation, and 3. Determination of the presence of radon daughters on the adult of Drosophila. It is necessary to point out that all the experiments were made by triplicate and in each one of them was placed detectors in preset places. Those obtained results are presented inside the 4 charts included in the present work. (Author)

  18. The Arts and Australian Education: Realising Potential. Australian Education Review No. 58

    Science.gov (United States)

    Ewing, Robyn

    2011-01-01

    Australian Education Review (AER) 58 surveys the international and national research on the role and effect of arts-rich programming in schools and in the broader community, and examines the policies and practices that inhibit or support these initiatives. It puts the case that embedding the Arts in learning would be a powerful catalyst for…

  19. Australian Apprentice & Trainee Statistics: Electrical and Electronics Trades, 1995 to 1999. Australian Vocational Education & Training.

    Science.gov (United States)

    National Centre for Vocational Education Research, Leabrook (Australia).

    Statistics regarding Australians participating in apprenticeships and traineeships in the electrical and electronics trades in 1995-1999 were reviewed to provide an indication of where skill shortages may be occurring or will likely occur in relation to the following occupations: electrical engineering associate professional; electronics…

  20. The Australian-Ness of Curriculum Jigsaws: Where Does Environmental Education Fit?

    Science.gov (United States)

    Gough, Annette

    2011-01-01

    This paper reviews Australian Government actions related to environmental education, particularly in the past decade, and examines the actions forthcoming from two national action plans (Environment Australia, 2000 and DEWHA, 2009), the implementation strategy for the Decade of ESD (DEWHA, 2006) and developments related to the Australian…

  1. AcEST: BP920910 [AcEST

    Lifescience Database Archive (English)

    Full Text Available tr|Q9XZR7|Q9XZR7_DROBU Protease, reverse transcriptase, ribonuclease H, integrase (Fragment) OS=Drosophila buzz...quen... 30 6.3 >tr|Q9XZR7|Q9XZR7_DROBU Protease, reverse transcriptase, ribonuclease H, integrase (Fragment) OS=Drosophila buzz...n 2 (Clone Db314) OS=Drosophila buzzatii GN=pol PE=4 SV=1 Length = 754 Score = 30.0 bits (66), Expect(2) = 1

  2. Philosophy and Ethics in Western Australian Secondary Schools

    Science.gov (United States)

    Millett, Stephan; Tapper, Alan

    2014-01-01

    The introduction of Philosophy and Ethics to the Western Australian Certificate of Education courses in 2008 brought philosophy into the Western Australian secondary school curriculum for the first time. How philosophy came to be included is part of a larger story about the commitment and perseverance of a relatively small number of Australian…

  3. What Do We Know about the Chancellors of Australian Universities?

    Science.gov (United States)

    O'Meara, Bernard; Petzall, Stanley

    2008-01-01

    This research attempts to explore the key social characteristics and demographics of Australian chancellors to determine who they are and where they come from. The chancellor of an Australian university wields an enormous amount of power, from overseeing the appointment of the Vice-Chancellor (VC) to fulfilling various statutory requirements.…

  4. Redefining & Leading the Academic Discipline in Australian Universities

    Science.gov (United States)

    Harkin, Damien G.; Healy, Annah H.

    2013-01-01

    Disciplines have emerged as an alternative administrative structure to departments or schools in Australian universities. We presently investigate the pattern of discipline use and by way of case study examine a role for distributed leadership in discipline management. Over forty per cent of Australian universities currently employ disciplines,…

  5. Cultural Patterns of Metacognitive Guidance in Australian Homes

    Science.gov (United States)

    Sanagavarapu, Prathyusha

    2008-01-01

    This article provides insight into the cultural patterns of metacognitive guidance that occurs among children and mothers in selected Australian homes. Fourteen Anglo Australian and eight immigrant Indian (Telugu) mothers' interactions with their 4-year-old male and female children on a puzzle-solving task were videotaped. Mother-child dyads'…

  6. Commonwealth Infrastructure Funding for Australian Universities: 2004 to 2011

    Science.gov (United States)

    Koshy, Paul; Phillimore, John

    2013-01-01

    This paper provides an overview of recent trends in the provision of general infrastructure funding by the Commonwealth for Australian universities (Table A providers) over the period 2004 to 2011. It specifically examines general infrastructure development and excludes funding for research infrastructure through the Australian Research Council or…

  7. Living Smart Homes: A Pilot Australian Sustainability Education Programme

    Science.gov (United States)

    Miller, Evonne; Buys, Laurie; Bell, Lorraine

    2009-01-01

    This article documents the rationale and experience of a pilot Australian sustainability education programme, "Living Smart Homes" (LSH) based on a community-based social marketing model. Inspired by the Australian "Land for Wildlife" scheme, LSH is designed to engage homeowners with sustainable practices through face-to-face workshops, an…

  8. The Teaching of First Year Economics in Australian Universities*

    OpenAIRE

    Nilss Olekalns

    2002-01-01

    This paper surveys current pedagogical practice in the teaching of introductory macroeconomics and microeconomics in Australian universities. Survey results are presented detailing lecturers’ approaches to their teaching over 2001 and other aspects of their teaching environment. A comparison of the content and methodology of the main textbooks used in Australian introductory economic courses is also presented.

  9. An Australian Story: School Sustainability Education in the Lucky Country

    Science.gov (United States)

    Salter, Zarin; Venville, Grady; Longnecker, Nancy

    2011-01-01

    This paper documents a case study involving a Perth primary school accompanied on its sustainability journey by Millennium Kids Inc, a local not-for-profit community organisation. Tension between the school's sustainability focus, its prestige as an elite private school and a "lucky country" mentality frames the Australian-ness of this…

  10. Educational and Institutional Flexibility of Australian Educational Software

    Science.gov (United States)

    Shurville, Simon; O'Grady, Thomas; Mayall, Peter

    2008-01-01

    Purpose: This paper aims to provide context for papers in this special issue on Australasian e-learning. The paper aims to examine the background to Australian flexible and transnational education and to evaluate the educational and intuitional flexibility of three typical products of the Australian educational software industry.…

  11. Teacher Transculturalism and Cultural Difference: Addressing Racism in Australian Schools

    Science.gov (United States)

    Casinader, Niranjan R.; Walsh, Lucas

    2015-01-01

    The increasing cultural diversity of students in Australia's schools is one of the salient changes in education over the last 30 years. In 2011, nearly half of all Australians had one or more parents born overseas, with migration from China, the Indian subcontinent and Africa increasing during the early 2000s (Australian Bureau of Statistics,…

  12. The Sleep Patterns and Well-Being of Australian Adolescents

    Science.gov (United States)

    Short, Michelle A.; Gradisar, Michael; Lack, Leon C.; Wright, Helen R.; Dohnt, Hayley

    2013-01-01

    Aim: Adolescent sleep patterns vary between countries, and these differences influence adolescent functioning and well-being. The present study provides data on the sleep and well-being of Australian adolescents. Methods: 385 adolescents aged 13-18 years were recruited from 8 South Australian schools spanning the socio-economic spectrum.…

  13. Civic Engagement and the Arts and Humanities: An Australian Perspective

    Science.gov (United States)

    Jones, Adrian

    2015-01-01

    An Australian scholar in the Arts and Humanities responds to recent US models emphasizing civic-engaged learning as a way to renew the humanities in undergraduate education. Policy contexts and curriculum initiatives of kindred trends in recent Australian undergraduate education in the humanities are contrasted in this essay. The Australian…

  14. The "Paradox of Interdisciplinarity" in Australian Research Governance

    Science.gov (United States)

    Woelert, Peter; Millar, Victoria

    2013-01-01

    This paper identifies what can be called the "paradox of interdisciplinarity" (Weingart 2000) in Australian higher education research governance and explores some of its constitutive dimensions. In the Australian context, the paradox of interdisciplinarity primarily concerns the proliferation of a programmatic discourse of…

  15. Training at the Australian School of Nuclear Technology

    International Nuclear Information System (INIS)

    The Australian School of Nuclear Technology was founded in 1964 as a joint enterprise of the Australian Atomic Energy Commission and the University of New South Wales to support nuclear developments primarily in Australia. However, ASNT has developed into an important centre for nuclear science and technology training within the South East Asian Region with participants also attending from countries outside this Region. (author)

  16. Training at the Australian School of Nuclear Technology

    International Nuclear Information System (INIS)

    The Australian School of Nuclear Technology (ASNT) was founded in 1964 as a joint enterprise of the Australian Atomic Energy Commission and the University of New South Wales to support nuclear developments primarily in Australia. However, ASNT has developed into an important centre for nuclear science and technology training within the South East Asian Region with participants also attending from countries outside this Region

  17. Australian Higher Education Reforms--Unification or Diversification?

    Science.gov (United States)

    Coombe, Leanne

    2015-01-01

    The higher education policy of the previous Australian government aimed to achieve an internationally competitive higher education sector while expanding access opportunities to all Australians. This policy agenda closely reflects global trends that focus on achieving both quality and equity objectives. In this paper, the formulation and…

  18. A Reconceptualisation of "Knowing Asia" in Australian Education

    Science.gov (United States)

    Salter, Peta

    2015-01-01

    Since 1969, over 60 Australian government and non-government policies, documents, committees, working parties and organisations have explored the need to "know Asia". In schools, this engagement is conceptualised as "Asia literacy" and disseminated in the emerging Australian Curriculum through the cross-curriculum priority…

  19. Teaching Australian Football in Physical Education: Constraints Theory in Practice

    Science.gov (United States)

    Pill, Shane

    2013-01-01

    This article outlines a constraints-led process of exploring, modifying, experimenting, adapting, and developing game appreciation known as Game Sense (Australian Sports Commission, 1997; den Duyn, 1996, 1997) for the teaching of Australian football. The game acts as teacher in this constraints-led process. Rather than a linear system that…

  20. Dementia risk factors for Australian baby boomers

    Directory of Open Access Journals (Sweden)

    Peter K. Panegyres

    2010-07-01

    Full Text Available Baby boomers are individuals born in the years 1946 to 1965. The objective of this paper was to define the risk factors for dementia and Alzheimer’s disease (AD and their relevance to Australian baby boomers, with the aim of providing evidence-based guidelines for dementia prevention. A series of PubMed searches (1994-2010 were conducted with relevant key words. Data was included from the Australian Bureau of Statistics (ABS in relation to baby boomers in Australia. Article titles and abstracts were assessed by two reviewers for inclusion. Searches through ABS revealed no specific study on baby boomers at a national level; information was only available for Western Australia, South Australia and Queensland. A number of genetic and non-genetic risk factors for dementia were identified most of which remain controversial and require further study. We did not identify significant differences in the prevalence and incidence of dementia in those under 65 years in Queensland, South Australia and Western Australia. There were no correlations of risk factors and dementia between the Australian states. Modification of risk factors has not been proven to reduce the incidence and prevalence of dementia and AD in baby boomers. Nevertheless, on available evidence, we recommend: i active management of cardiovascular risk factors such as hypertension; ii the encouragement of a healthy lifestyle (eg, weight reduction, exercise as offering the best pathways to reduce the emerging dementia risk for baby boomers. The implications are that activities promoting a healthy heart might lead to a healthy brain and help to prevent dementia.

  1. The Australian Natural Disaster Resilience Index

    Science.gov (United States)

    Thoms, Martin

    2016-04-01

    The Australian Natural Disaster Resilience Index Martin Thoms, Melissa Parsons, Phil Morley Bushfire and Natural Hazards Cooperative Research Centre, Geography and Planning, University of New England, Armidale NSW 2351, Australia. Natural hazard management policy directions in Australia - and indeed internationally - are increasingly being aligned to ideas of resilience. Resilience to natural hazards is the ability of individuals and communities to cope with disturbance and adversity and to maintain adaptive behaviour. Operationalizing the measurement and assessment of disaster resilience is often undertaken using a composite index, but this exercise is yet to be undertaken in Australia. The Australian Natural Disaster Resilience Index is a top-down, national scale assessment of the resilience of communities to natural hazards. Resilience is assessed based on two sets of capacities: coping and adaptive capacities. Coping capacity relates to the factors influencing the ability of a community to prepare for, absorb and recover from a natural hazard event. Adaptive capacity relates to the arrangements and processes that enable adjustment through learning, adaptation and transformation. Indicators are derived under themes of social character, economic capital, infrastructure and planning, emergency services, community capital, information and engagement and governance/leadership/policy, using existing data sets (e.g. census data) or evaluation of policy and procedure (e.g. disaster management planning). A composite index of disaster resilience is then computed for each spatial division, giving national scale coverage. The results of the Australian Natural Disaster Resilience Index will be reported in a State of Disaster Resilience report, due in 2018. The index is co-designed with emergency service agencies, and will support policy development, planning, community engagement and emergency management.

  2. Patient Experience of Australian General Practices.

    Science.gov (United States)

    Narayanan, Ajit; Greco, Michael

    2016-03-01

    The number of data-based research articles focusing on patient sociodemographic profiling and experience with healthcare practices is still relatively small. One of the reasons for this relative lack of research is that categorizing patients into different demographic groups can lead to significant reductions in sample numbers for homogeneous subgroups. The aim of this article is to identify problems and issues when dealing with big data that contains information at two levels: patient experience of their general practice, and scores received by practices. The Practice Accreditation and Improvement Survey (PAIS) consisting of 27 five-point Likert items and 11 sociodemographic questions is a Royal Australian College of General Practitioners (RACGP)-endorsed instrument for seeking patient views as part of the accreditation of Australian general practices. The data were collected during the 3-year period May 2011-July 2014, during which time PAIS was completed for 3734 individual general practices throughout Australia involving 312,334 anonymous patients. This represents over 60% of practices in Australia, and ∼75% of practices that undergo voluntary accreditation. The sampling method for each general practice was convenience sampling. The results of our analysis show how sociodemographic profiles of Australian patients can affect their ratings of practices and also how the location of the practice (State/Territory, remote access area) can affect patient experience. These preliminary findings can act as an initial set of results against which future studies in patient experience trends can be developed and measured in Australia. Also, the methods used in this article provide a methodological framework for future patient experience researchers to use when dealing with data that contain information at two levels, such as the patient and practice. Finally, the outcomes demonstrate that different subgroups can experience healthcare provision differently, especially

  3. First record of spotted wing drosophila Drosophila suzukii (Diptera: Drosophilidae in Montenegro

    Directory of Open Access Journals (Sweden)

    Snježana Hrnčić

    2015-01-01

    Full Text Available The spotted wing drosophila Drosophila suzukii Matsumura (Diptera: Drosophilidae is an invasive pest originating from Southeast Asia. It was detected for the first time in Europe in 2008 (Spain and Italy and subsequently in other European countries. It is a highly polyphagous pest that infests healthy, ripening fruit and presents a serious threat to fruit production, particularly of soft skinned fruit. In the first half of October 2013, a new fruit fly species was unexpectedly detected in Tephri traps baited with the three-component female-biased attractant BioLure that is regularly used for monitoring the Mediterranean fruit fly Ceratitis capitata Wiedem. (Diptera: Tephritidae in Montenegro. Brief visual inspection identified the new species as the spotted wing drosophila D. suzukii. The pest was first recorded in several localities on the Montenegrin seacoast around Boka Kotor Bay. After the finding, all Drosophila specimens were collected from traps for further laboratory observation. A quick follow-up monitoring of other Tephri traps was carried out within the next few days on the rest of the seacoast (localities from Tivat to Ulcinj. Additionally, Tephri traps were set up around Lake Skadar and in the city of Podgorica, as well as on fresh fruit markets in Podgorica. The results of this preliminary study showed that D. suzukii was present in all surveyed locations and adults were captured until late December. Both sexes were found in traps with BioLure. Our data show that D. suzukii is present in southern parts of Montenegro and there is a serious threat of its further spreading, particularly towards northern parts of the country where the main raspberry and blueberry production is placed. The results also show that Tephri traps baited with BioLure can be used for detection and monitoring of spotted wing drosophila.

  4. Taxonomy Icon Data: Australian echidna [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available Australian echidna Tachyglossus aculeatus Chordata/Vertebrata/Mammalia/Prototheria Tachygloss...us_aculeatus_L.png Tachyglossus_aculeatus_NL.png Tachyglossus_aculeatus_S.png Tachyglossus_aculeat...us_NS.png http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Tachyglossus+aculeatus&t=L http://biosciencedbc.j...p/taxonomy_icon/icon.cgi?i=Tachyglossus+aculeatus&t=NL http://biosciencedbc.jp/ta...xonomy_icon/icon.cgi?i=Tachyglossus+aculeatus&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Tachyglossus+aculeatus&t=NS ...

  5. Markets, Distance Education, and Australian Higher Education

    Directory of Open Access Journals (Sweden)

    Ted Nunan

    2005-03-01

    Full Text Available The paper argues that the Australian university system is unstable. There will be significant change as government implements its reform agenda and even more radical change if it moves to new deregulation. The role of distance education in university education needs to be analyzed against this ‘market’ agenda of government in terms of characteristics of markets and market behavior. After a scan of the current role, the paper looks at two scenarios (regulated and deregulated for distance education in university teaching and learning in Australia.

  6. The State of the Australian Middle Class

    Directory of Open Access Journals (Sweden)

    Clive Hamilton

    2008-09-01

    Full Text Available There is a widespread view that the middle class in Australia is doing it tough, that they arefinding it increasingly difficult to maintain a decent standard of living and are suffering frommortgage stress. Indeed, some media reports have announced the end of the middle classdream.This paper tests a number of these popular views against the statistical data. It asks whetherthe typical Australian family can be said to be struggling? Are mortgages creating severeproblems for middle-class families? Is the middle class shrinking? Are families copingfinancially only because wives are going out to work?

  7. Nuclear geophysiology: Isotopes in Australian environmental analysis

    International Nuclear Information System (INIS)

    Lovelock introduced the term 'geophysiology' to describe the holistic study of the Earth systems. By analogy with medicine, and the corresponding field of nuclear medicine, 'nuclear geophysiology' describes the application of nuclear techniques to Earth system science. Injections of radioisotopes into the Earth's systems occur naturally and continuously, while artificial radionuclides have been injected at times as a result of human activities. Here, we provide some examples of Australian investigations into the physiology of the Earth derived from the study of these isotopes. (author)

  8. Building the Clinical Bridge: An Australian Success

    Directory of Open Access Journals (Sweden)

    Marianne Wallis

    2012-01-01

    Full Text Available Nursing effectiveness science includes primary, secondary, and translational, clinically focused research activities which aim to improve patient or client outcomes. It is imperative, for the successful conduct of a program of nursing effectiveness science, that a clinical bridge is established between academic and healthcare service facilities. An Australian example of the development of a robust clinical bridge through the use of jointly funded positions at the professorial level is outlined. In addition, an analysis of the practical application of Lewin’s model of change management and the contribution of both servant and transformational leadership styles to the bridge building process is provided.

  9. Building the clinical bridge: an Australian success.

    Science.gov (United States)

    Wallis, Marianne; Chaboyer, Wendy

    2012-01-01

    Nursing effectiveness science includes primary, secondary, and translational, clinically focused research activities which aim to improve patient or client outcomes. It is imperative, for the successful conduct of a program of nursing effectiveness science, that a clinical bridge is established between academic and healthcare service facilities. An Australian example of the development of a robust clinical bridge through the use of jointly funded positions at the professorial level is outlined. In addition, an analysis of the practical application of Lewin's model of change management and the contribution of both servant and transformational leadership styles to the bridge building process is provided.

  10. Stress in the Indo-Australian plate

    Science.gov (United States)

    Cloetingh, Sierd; Wortel, Rinus

    1986-12-01

    We modelled the state of stress in the Indo-Australian plate in order to investigate quantitatively variations observed in tectonic style. The numerical procedure incorporates the dependence of slab pull and ridge push on the age of the oceanic lithosphere. Estimates are presented for the average net resistive forces at the Himalayan collision zone, the suction force acting on the overriding Indo-Australian plate segment at the Tonga-Kermadec trench and the drag at the base of the lithosphere. Our modelling shows a concentration of compressive stresses of the order of 3-5 kbar in the Ninetyeast Ridge area; the effects of the compressive resistance associated with Himalayan collision and subduction of young lithosphere off the northern part of the Sunda arc are focused in this region. The stress field as calculated gives a consistent explanation for the observed concentration of seismic activity (Stein and Okal, 1978) and significant deformation in the oceanic crust (Weissel et al., 1980; McAdoo and Sandwell, 1985) in the area. The calculated stress field in the area adjacent to the Southeast and Central India ridges is characterized by tension parallel to the spreading axis. This explains the concentration of near-ridge normal faulting seismicity (with T-axes subparallel to the spreading ridge) in the Indian Ocean as recently observed by Bergman et al. (1984) and Wiens and Stein (1984). The regional stress field along the strike of the Sunda arc varies from compression seaward of and parallel to the Sumatra trench segment, to tension perpendicular to the Java-Flores segment. This explains the selective occurrence of well developed grabens seaward off the Java-Flores segment of the trench, observed by Hilde (1983). Our modelling shows that the observed rotation of the stress field (Denham et al., 1979) in the Australian continent is mainly the consequence of its geographic position relative to the surrounding trench segments and the variations of the forces acting

  11. A new era in Australian migration policy.

    Science.gov (United States)

    Birrell, R

    1984-01-01

    The discussion traces the evolution of Australian migration policy since 1975, arguing that the primary factor shaping policy has been interparty competition for influence within Australia's ethnic communities. Since late 1975 when the Liberal/National Country Party (LibNCP) Conservative Government returned to power, Australian immigration policy has moved in different directions from the previous post World War II experience. The demographic implications have been profound. In 1975 the LibNCP government returned to office committed to restoring an active migration program. By 1980-81 it had largely succeeded in this numerical goal. Australia's migration growth rate at .82% of the total population exceeded almost all other Western society. What was new, in comparison to previous policy, was the migrant selection system and source countries. By the time the government lost office in March 1983, family reunion had become the major migration program souce and Asia was rapidly becoming the dominant place of migrant origin. This emphasis on family reunion was not intended by government immigration planners but was a product of domestic political change and resultant new influences over migration policy. As to the increasing Asian component, it has mainly been an unintended consequence of the expansion in the family reunion program. Although the liberalization of family reunion eligibility has largely been designed to appease the major Southern European ethnic communities, few applications have been forthcoming from these countries. Asian applicants have been numerous. Labor government policy since March 1983 has shown remarkable continuity with that of the LibNCP both in its selection system and in the size of the migrant intake. The motivation for the commitment to immigration derived first from longstanding traditions within the Australian business community that Australia's economic growth and dynamism depended on rapid population growth. More specifically there

  12. Ion channels to inactivate neurons in Drosophila

    Directory of Open Access Journals (Sweden)

    James J L Hodge

    2009-08-01

    Full Text Available Ion channels are the determinants of excitability; therefore, manipulation of their levels and properties provides an opportunity for the investigator to modulate neuronal and circuit function. There are a number of ways to suppress electrical activity in Drosophila neurons, for instance, over-expression of potassium channels (i.e. Shaker Kv1, Shaw Kv3, Kir2.1 and DORK that are open at resting membrane potential. This will result in increased potassium efflux and membrane hyperpolarisation setting resting membrane potential below the threshold required to fire action potentials. Alternatively over-expression of other channels, pumps or co-transporters that result in a hyperpolarised membrane potential will also prevent firing. Lastly, neurons can be inactivated by, disrupting or reducing the level of functional voltage-gated sodium (Nav1 paralytic or calcium (Cav2 cacophony channels that mediate the depolarisation phase of action potentials. Similarly, strategies involving the opposite channel manipulation should allow net depolarisation and hyperexcitation in a given neuron. These changes in ion channel expression can be brought about by the versatile transgenic (i.e. Gal4/UAS based systems available in Drosophila allowing fine temporal and spatial control of (channel transgene expression. These systems are making it possible to electrically inactivate (or hyperexcite any neuron or neural circuit in the fly brain, and much like an exquisite lesion experiment, potentially elucidate whatever interesting behaviour or phenotype each network mediates. These techniques are now being used in Drosophila to reprogram electrical activity of well-defined circuits and bring about robust and easily quantifiable changes in behaviour, allowing different models and hypotheses to be rapidly tested.

  13. Role of spectraplakin in Drosophila photoreceptor morphogenesis.

    Directory of Open Access Journals (Sweden)

    Uyen Ngoc Mui

    Full Text Available BACKGROUND: Crumbs (Crb, a cell polarity gene, has been shown to provide a positional cue for the apical membrane domain and adherens junction during Drosophila photoreceptor morphogenesis. It has recently been found that stable microtubules in developing Drosophila photoreceptors were linked to Crb localization. Coordinated interactions between microtubule and actin cytoskeletons are involved in many polarized cellular processes. Since Spectraplakin is able to bind both microtubule and actin cytoskeletons, the role of Spectraplakin was analyzed in the regulations of apical Crb domain in developing Drosophila photoreceptors. METHODOLOGY/PRINCIPAL FINDINGS: The localization pattern of Spectraplakin in developing pupal photoreceptors showed a unique intracellular distribution. Spectraplakin localized at rhabdomere terminal web which is at the basal side of the apical Crb or rhabdomere, and in between the adherens junctions. The spectraplakin mutant photoreceptors showed dramatic mislocalizations of Crb, adherens junctions, and the stable microtubules. This role of Spectraplakin in Crb and adherens junction regulation was further supported by spectraplakin's gain-of-function phenotype. Spectraplakin overexpression in photoreceptors caused a cell polarity defect including dramatic mislocalization of Crb, adherens junctions and the stable microtubules in the developing photoreceptors. Furthermore, a strong genetic interaction between spectraplakin and crb was found using a genetic modifier test. CONCLUSIONS/SIGNIFICANCE: In summary, we found a unique localization of Spectraplakin in photoreceptors, and identified the role of spectraplakin in the regulation of the apical Crb domain and adherens junctions through genetic mutational analysis. Our data suggest that Spectraplakin, an actin-microtubule cross-linker, is essential in the apical and adherens junction controls during the photoreceptors morphogenesis.

  14. The Relationship between Self-Esteem and Parenting Style: A Cross-Cultural Comparison of Australian and Vietnamese Australian Adolescents.

    Science.gov (United States)

    Herz, Lara; Gullone, Eleonora

    1999-01-01

    Studied the relationship between self-esteem and parenting style with 118 Vietnamese Australian and 120 Anglo-Australian adolescents. As expected, parenting characterized by high levels of overprotection and low levels of acceptance related negatively with self-esteem for both samples of adolescents. (SLD)

  15. Acetylation regulates Jun protein turnover in Drosophila.

    Science.gov (United States)

    Zhang, Daoyong; Suganuma, Tamaki; Workman, Jerry L

    2013-11-01

    C-Jun is a major transcription factor belonging to the activating protein 1 (AP-1) family. Phosphorylation has been shown to be critical for c-Jun activation and stability. Here, we report that Jra, the Drosophila Jun protein, is acetylated in vivo. We demonstrate that the acetylation of Jra leads to its rapid degradation in response to osmotic stress. Intriguingly, we also found that Jra phosphorylation antagonized its acetylation, indicating the opposite roles of acetylation and phosphorylation in Jra degradation process under osmotic stress. Our results provide new insights into how c-Jun proteins are precisely regulated by the interplay of different posttranslational modifications.

  16. The Drosophila melanogaster circadian pacemaker circuit

    Indian Academy of Sciences (India)

    Vasu Sheeba

    2008-12-01

    As an experimental model system, the fruit fly Drosophila melanogaster has been seminal in shaping our understanding of the circadian clockwork. The wealth of genetic tools at our disposal over the past four decades has enabled discovery of the genetic and molecular bases of circadian rhythmicity. More recently, detailed investigation leading to the anatomical, neurochemical and electrophysiological characterization of the various neuronal subgroups that comprise the circadian machinery has revealed pathways through which these neurons come together to act as a neuronal circuit. Thus the D. melanogaster circadian pacemaker circuit presents a relatively simple and attractive model for the study of neuronal circuits and their functions.

  17. Hypergravity-induced altered behavior in Drosophila

    Science.gov (United States)

    Hosamani, Ravikumar; Wan, Judy; Marcu, Oana; Bhattacharya, Sharmila

    2012-07-01

    Microgravity and mechanical stress are important factors of the spaceflight environment, and affect astronaut health and behavior. Structural, functional, and behavioral mechanisms of all cells and organisms are adapted to Earth's gravitational force, 1G, while altered gravity can pose challenges to their adaptability to this new environment. On ground, hypergravity paradigms have been used to predict and complement studies on microgravity. Even small changes that take place at a molecular and genetic level during altered gravity may result in changes in phenotypic behavior. Drosophila provides a robust and simple, yet very reliable model system to understand the complexity of hypergravity-induced altered behavior, due to availability of a plethora of genetic tools. Locomotor behavior is a sensitive parameter that reflects the array of molecular adaptive mechanisms recruited during exposure to altered gravity. Thus, understanding the genetic basis of this behavior in a hypergravity environment could potentially extend our understanding of mechanisms of adaptation in microgravity. In our laboratory we are trying to dissect out the cellular and molecular mechanisms underlying hypergravity-induced oxidative stress, and its potential consequences on behavioral alterations by using Drosophila as a model system. In the present study, we employed pan-neuronal and mushroom body specific knock-down adult flies by using Gal4/UAS system to express inverted repeat transgenes (RNAi) to monitor and quantify the hypergravity-induced behavior in Drosophila. We established that acute hypergravity (3G for 60 min) causes a significant and robust decrease in the locomotor behavior in adult Drosophila, and that this change is dependent on genes related to Parkinson's disease, such as DJ-1α , DJ-1β , and parkin. In addition, we also showed that anatomically the control of this behavior is significantly processed in the mushroom body region of the fly brain. This work links a molecular

  18. Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine

    DEFF Research Database (Denmark)

    Knecht, Wolfgang; Mikkelsen, N.E.; Clausen, A.R.;

    2009-01-01

    Drosophila melanogaster multisubstrate deoxyribonucleoside kinase (Dm-dNK) can additionally sensitize human cancer cell lines towards the anti-cancer drug gemcitabine. We show that this property is based on the Dm-dNK ability to efficiently phosphorylate gemcitabine. The 2.2 angstrom resolution s...... structure of DmdNK in complex with gemcitabine shows that the residues Tyr70 and Arg105 play a crucial role in the firm positioning of gemcitabine by extra interactions made by the fluoride atoms. This explains why gemcitabine is a good substrate for Dm-dNK(....

  19. Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine

    Energy Technology Data Exchange (ETDEWEB)

    Knecht, Wolfgang [BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby (Denmark); Mikkelsen, Nils Egil [Department of Molecular Biology, Swedish University of Agricultural Sciences, Biomedical Centre, SE-751 24 Uppsala (Sweden); Clausen, Anders Ranegaard [Cell and Organism Biology, Lund University, Soelvegatan 35, SE-22362 Lund (Sweden); Willer, Mette [ZGene A/S, Agern Alle 7, DK-2970 Horsholm (Denmark); Eklund, Hans [Department of Molecular Biology, Swedish University of Agricultural Sciences, Biomedical Centre, SE-751 24 Uppsala (Sweden); Gojkovic, Zoran [ZGene A/S, Agern Alle 7, DK-2970 Horsholm (Denmark); Piskur, Jure, E-mail: Jure.Piskur@cob.lu.se [BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby (Denmark); Cell and Organism Biology, Lund University, Soelvegatan 35, SE-22362 Lund (Sweden)

    2009-05-01

    Drosophila melanogaster multisubstrate deoxyribonucleoside kinase (Dm-dNK) can additionally sensitize human cancer cell lines towards the anti-cancer drug gemcitabine. We show that this property is based on the Dm-dNK ability to efficiently phosphorylate gemcitabine. The 2.2 A resolution structure of Dm-dNK in complex with gemcitabine shows that the residues Tyr70 and Arg105 play a crucial role in the firm positioning of gemcitabine by extra interactions made by the fluoride atoms. This explains why gemcitabine is a good substrate for Dm-dNK.

  20. ‘Peer pressure’ in larval Drosophila?

    OpenAIRE

    Thomas Niewalda; Ines Jeske; Birgit Michels; Bertram Gerber

    2014-01-01

    ABSTRACT Understanding social behaviour requires a study case that is simple enough to be tractable, yet complex enough to remain interesting. Do larval Drosophila meet these requirements? In a broad sense, this question can refer to effects of the mere presence of other larvae on the behaviour of a target individual. Here we focused in a more strict sense on ‘peer pressure’, that is on the question of whether the behaviour of a target individual larva is affected by what a surrounding group ...

  1. A connectionist model of the Drosophila blastoderm

    Energy Technology Data Exchange (ETDEWEB)

    Reinitz, J. (Columbia Univ., New York, NY (USA). Dept. of Biological Sciences); Mjolsness, E. (Yale Univ., New Haven, CT (USA). Dept. of Computer Science); Sharp, D.H. (Los Alamos National Lab., NM (USA). Theoretical Div.)

    1990-11-01

    The authors present a phenomenological modeling framework for development, and apply it to the network of segmentation genes operating in the blastoderm of Drosophila. Their purpose is to provide a systematic method for discovering and expressing correlations in experimental data on gene expression and other developmental processes. The modeling framework is based on a connectionist or neural net dynamics for biochemical regulators, coupled to grammatical rules which describe certain features of the birth, growth, and death of cells, synapses and other biological entities. They present preliminary numerical results regarding regulatory interactions between the genes Kruppel and knirps that demonstrate the potential utility of the model. 14 refs., 5 figs.

  2. Characterization of maltase clusters in the genus Drosophila.

    Science.gov (United States)

    Gabriško, Marek; Janeček, Stefan

    2011-01-01

    To reveal evolutionary history of maltase gene family in the genus Drosophila, we undertook a bioinformatics study of maltase genes from available genomes of 12 Drosophila species. Molecular evolution of a closely related glycoside hydrolase, the α-amylase, in Drosophila has been extensively studied for a long time. The α-amylases were even used as a model of evolution of multigene families. On the other hand, maltase, i.e., the α-glucosidase, got only scarce attention. In this study, we, therefore, investigated spatial organization of the maltase genes in Drosophila genomes, compared the amino acid sequences of the encoded enzymes and analyzed the intron/exon composition of orthologous genes. We found that the Drosophila maltases are more numerous than previously thought (ten instead of three genes) and are localized in two clusters on two chromosomes (2L and 2R). To elucidate the approximate time line of evolution of the clusters, we estimated the order and dated duplication of all the 10 genes. Both clusters are the result of ancient series of subsequent duplication events, which took place from 352 to 61 million years ago, i.e., well before speciation to extant Drosophila species. Also observed was a remarkable intron/exon composition diversity of particular maltase genes of these clusters, probably a result of independent intron loss after duplication of intron-rich gene ancestor, which emerged well before speciation in a common ancestor of all extant Drosophila species.

  3. The potential for an Australian uranium industry

    International Nuclear Information System (INIS)

    The production of uranium and its part upgrading to enriched uranium for export could be equivalent to 20-25 per cent of Australia's future export income from coal. Australia could be supplying 15,000 tonnes U/yr. and enrichment services of 2.5 million SWU/yr. by 2000. The principles of nuclear energy, nuclear power reactors and the nuclear fuel cycle are described and the relationship between nuclear power and the requirements for uranium and the other steps in the fuel cycle is discussed. Estimates are given of the future world supply-demand balance for each step in the fuel cycle. A survey is made of world uranium resources and fuel cycle upgrading facilities. The costs of production and pricing are assessed in relation to the potential for an Australian industry. Comments are made on the possibility that Australia could provide the repository for both low-level radioactive waste from small countries and the bulk of the world's high level waste. The impact of a uranium industry on the Australian economy is discussed

  4. Injury profile of amateur Australian rules footballers.

    Science.gov (United States)

    Shawdon, A; Brukner, P

    1994-01-01

    Australian Rules Football is played by numerous young Australians throughout winter each year. There have been a number of studies on professional and semi-professional footballers, establishing the nature and frequency of injuries within this football code. Medical cover of an amateur football club over the 1993 season allowed detailed recording of injuries over this period. The data collected revealed a markedly different injury profile to that seen previously. The injury rate in this study was 96 per 1000 player hours. The most common injury was concussion (15%), with hand fractures next most frequent (13.5%). The lower limb was the most common site of injury, with head and neck second and upper limb third. Injuries with an overuse component were seen less commonly in the amateur group while traumatic injuries were more frequent. The time allocated by amateur footballers to their sport is less than professional players, quite aside from the difference in skill level attained. Overuse injuries may be correspondingly much less frequent on a time basis alone. The increased incidence of traumatic injuries is postulated to be a manifestation of both less well developed skills and possibly less available and effective preventative measures such as ankle strapping and tape supplies. Considering the large number of young people playing amateur football and the significant time and cost of what are often relatively minor injuries, more work is required to establish what injuries are most common, and importantly, what measures can be taken to decrease their incidence. PMID:8665278

  5. Laterality enhances cognition in Australian parrots.

    Science.gov (United States)

    Magat, Maria; Brown, Culum

    2009-12-01

    Cerebral lateralization refers to the division of information processing in either hemisphere of the brain and is a ubiquitous trait among vertebrates and invertebrates. Given its widespread occurrence, it is likely that cerebral lateralization confers a fitness advantage. It has been hypothesized that this advantage takes the form of enhanced cognitive function, potentially via a dual processing mechanism whereby each hemisphere can be used to process specific types of information without contralateral interference. Here, we examined the influence of lateralization on problem solving by Australian parrots. The first task, a pebble-seed discrimination test, was designed for small parrot species that feed predominately on small seeds, which do not require any significant manipulation with the foot prior to ingestion. The second task, a string-pull problem, was designed for larger bodied species that regularly use their feet to manipulate food objects. In both cases, strongly lateralized individuals (those showing significant foot and eye biases) outperformed less strongly lateralized individuals, and this relationship was substantially stronger in the more demanding task. These results suggest that cerebral lateralization is a ubiquitous trait among Australian parrots and conveys a significant foraging advantage. Our results provide strong support for the enhanced cognitive function hypothesis.

  6. Patterns of multimorbidity in working Australians

    Directory of Open Access Journals (Sweden)

    Ng Shu-Kay

    2011-06-01

    Full Text Available Abstract Background Multimorbidity is becoming more prevalent. Previously-used methods of assessing multimorbidity relied on counting the number of health conditions, often in relation to an index condition (comorbidity, or grouping conditions based on body or organ systems. Recent refinements in statistical approaches have resulted in improved methods to capture patterns of multimorbidity, allowing for the identification of nonrandomly occurring clusters of multimorbid health conditions. This paper aims to identify nonrandom clusters of multimorbidity. Methods The Australian Work Outcomes Research Cost-benefit (WORC study cross-sectional screening dataset (approximately 78,000 working Australians was used to explore patterns of multimorbidity. Exploratory factor analysis was used to identify nonrandomly occurring clusters of multimorbid health conditions. Results Six clinically-meaningful groups of multimorbid health conditions were identified. These were: factor 1: arthritis, osteoporosis, other chronic pain, bladder problems, and irritable bowel; factor 2: asthma, chronic obstructive pulmonary disease, and allergies; factor 3: back/neck pain, migraine, other chronic pain, and arthritis; factor 4: high blood pressure, high cholesterol, obesity, diabetes, and fatigue; factor 5: cardiovascular disease, diabetes, fatigue, high blood pressure, high cholesterol, and arthritis; and factor 6: irritable bowel, ulcer, heartburn, and other chronic pain. These clusters do not fall neatly into organ or body systems, and some conditions appear in more than one cluster. Conclusions Considerably more research is needed with large population-based datasets and a comprehensive set of reliable health diagnoses to better understand the complex nature and composition of multimorbid health conditions.

  7. The "lessons" of the Australian "heroin shortage"

    Directory of Open Access Journals (Sweden)

    Gilmour Stuart

    2006-05-01

    Full Text Available Abstract Heroin use causes considerable harm to individual users including dependence, fatal and nonfatal overdose, mental health problems, and blood borne virus transmission. It also adversely affects the community through drug dealing, property crime and reduced public amenity. During the mid to late 1990s in Australia the prevalence of heroin use increased as reflected in steeply rising overdose deaths. In January 2001, there were reports of an unpredicted and unprecedented reduction in heroin supply with an abrupt onset in all Australian jurisdictions. The shortage was most marked in New South Wales, the State with the largest heroin market, which saw increases in price, dramatic decreases in purity at the street level, and reductions in the ease with which injecting drug users reported being able to obtain the drug. The abrupt onset of the shortage and a subsequent dramatic reduction in overdose deaths prompted national debate about the causes of the shortage and later international debate about the policy significance of what has come to be called the "Australian heroin shortage". In this paper we summarise insights from four years' research into the causes, consequences and policy implications of the "heroin shortage".

  8. Is there an Australian Pastoral Poetry?

    Directory of Open Access Journals (Sweden)

    Andrew Taylor

    2015-11-01

    Full Text Available Pastoral was common as a European literary genre from the Renaissance until the eighteenth century. It existed in other artistic forms as well, especially in the visual arts, and after its demise as a distinct genre elements of it persisted into the twentieth century, for example in music. With the colonial spread of European culture the pastoral influence also extended into other countries, with a mixed fate. Recently, the term Pastoral has come back into prominence in literature in English, not only in Great Britain but also, notably in the USA and Australia, with the growth of writing motivated by ecological involvement with the natural world, especially landscape. This has led to re-definitions of the term Pastoral in the last few decades. A number of Australian poets are looked at to see whether, and how, their writing about landscape might relate to, or incorporate elements of the Pastoral. The Australian poet John Kinsella, in particular, has been a widely published spokesperson for a new definition of Pastoral. His published works trace his move from a politically activist anti-colonialist redefinition of Pastoral towards a quieter, more harmonious, and essentially ethical engagement with the natural world.

  9. Genetic effects of plutonium in Drosophila. Final technical report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-07-01

    This three year project, initiated in 1987, involved the genetic effects of alpha radiations on Drosophila. This document represents the final technical report. Plutonium residue was used as the alpha source of radon gas. Spontaneous mutation frequency in the Drosophila stock was very low. In the experiments using alpha radiation from radon gas, radiation doses as low as 20R induced significant numbers of mutations, with higher numbers of mutations at higher doses. If X-ray induced mutation frequencies reported in the literature are used for comparison, it can be concluded that alpha radiation from radon gas induces at least 2 to 3 time more mutations in Drosophila.

  10. Deconstructing host-pathogen interactions in Drosophila

    Directory of Open Access Journals (Sweden)

    Ethan Bier

    2012-01-01

    Full Text Available Many of the cellular mechanisms underlying host responses to pathogens have been well conserved during evolution. As a result, Drosophila can be used to deconstruct many of the key events in host-pathogen interactions by using a wealth of well-developed molecular and genetic tools. In this review, we aim to emphasize the great leverage provided by the suite of genomic and classical genetic approaches available in flies for decoding details of host-pathogen interactions; these findings can then be applied to studies in higher organisms. We first briefly summarize the general strategies by which Drosophila resists and responds to pathogens. We then focus on how recently developed genome-wide RNA interference (RNAi screens conducted in cells and flies, combined with classical genetic methods, have provided molecular insight into host-pathogen interactions, covering examples of bacteria, fungi and viruses. Finally, we discuss novel strategies for how flies can be used as a tool to examine how specific isolated virulence factors act on an intact host.

  11. The smell of love in Drosophila

    Directory of Open Access Journals (Sweden)

    Anna B. eZiegler

    2013-04-01

    Full Text Available Odors are key sensory signals for social communication and food search in animals including insects. Drosophila melanogaster, is a powerful neurogenetic model commonly used to reveal molecular and cellular mechanisms involved in odorant detection. Males use olfaction together with other sensory modalities to find their mates. Here, we review known olfactory signals, their related olfactory receptors, and the corresponding neuronal architecture impacting courtship. OR67d receptor detects 11-cis-Vaccenyl Acetate (cVA, a male specific pheromone transferred to the female during copulation. Transferred cVA is able to reduce female attractiveness for other males after mating, and is also suspected to decrease male-male courtship. cVA can also serve as an aggregation signal, maybe through another OR. OR47b was shown to be activated by fly odors, and to enhance courtship depending on taste pheromones. IR84a detects phenylacetic acid (PAA and phenylacetaldehyde. These two odors are not pheromones produced by flies, but are present in various fly food sources. PAA enhances male courtship, acting as a food aphrodisiac. Drosophila males have thus developed complementary olfactory strategies to help them to select their mates.

  12. Behavioural reproductive isolation and speciation in Drosophila

    Indian Academy of Sciences (India)

    Punita Nanda; Bashisth Narayan Singh

    2012-06-01

    The origin of premating reproductive isolation continues to help elucidate the process of speciation and is the central event in the evolution of biological species. Therefore, during the process of species formation the diverging populations must acquire some means of reproductive isolation so that the genes from one gene pool are prevented from dispersing freely into a foreign gene pool. In the genus Drosophila, the phenomenon of behavioural reproductive isolation, which is an important type of premating (prezygotic) reproductive isolating mechanisms, has been extensively studied and interesting data have been documented. In many cases incomplete sexual isolation has been observed and the pattern and degree of isolation within and between the species have often been used to elucidate the phylogenetic relationships. The present review documents an overview of speciation mediated through behavioural incompatibility in different species groups of Drosophila with particular reference to the models proposed on the basis of one-sided ethological isolation to predict the direction of evolution. This study is crucial for understanding the mechanism of speciation through behavioural incompatibility and also for an understanding of speciation genetics in future prospects.

  13. Drosophila Porin/VDAC affects mitochondrial morphology.

    Directory of Open Access Journals (Sweden)

    Jeehye Park

    Full Text Available Voltage-dependent anion channel (VDAC has been suggested to be a mediator of mitochondrial-dependent cell death induced by Ca(2+ overload, oxidative stress and Bax-Bid activation. To confirm this hypothesis in vivo, we generated and characterized Drosophila VDAC (porin mutants and found that Porin is not required for mitochondrial apoptosis, which is consistent with the previous mouse studies. We also reported a novel physiological role of Porin. Loss of porin resulted in locomotive defects and male sterility. Intriguingly, porin mutants exhibited elongated mitochondria in indirect flight muscle, whereas Porin overexpression produced fragmented mitochondria. Through genetic analysis with the components of mitochondrial fission and fusion, we found that the elongated mitochondria phenotype in porin mutants were suppressed by increased mitochondrial fission, but enhanced by increased mitochondrial fusion. Furthermore, increased mitochondrial fission by Drp1 expression suppressed the flight defects in the porin mutants. Collectively, our study showed that loss of Drosophila Porin results in mitochondrial morphological defects and suggested that the defective mitochondrial function by Porin deficiency affects the mitochondrial remodeling process.

  14. Logical modelling of Drosophila signalling pathways.

    Science.gov (United States)

    Mbodj, Abibatou; Junion, Guillaume; Brun, Christine; Furlong, Eileen E M; Thieffry, Denis

    2013-09-01

    A limited number of signalling pathways are involved in the specification of cell fate during the development of all animals. Several of these pathways were originally identified in Drosophila. To clarify their roles, and possible cross-talk, we have built a logical model for the nine key signalling pathways recurrently used in metazoan development. In each case, we considered the associated ligands, receptors, signal transducers, modulators, and transcription factors reported in the literature. Implemented using the logical modelling software GINsim, the resulting models qualitatively recapitulate the main characteristics of each pathway, in wild type as well as in various mutant situations (e.g. loss-of-function or gain-of-function). These models constitute pluggable modules that can be used to assemble comprehensive models of complex developmental processes. Moreover, these models of Drosophila pathways could serve as scaffolds for more complicated models of orthologous mammalian pathways. Comprehensive model annotations and GINsim files are provided for each of the nine considered pathways.

  15. Selective anticancer agents suppress aging in Drosophila.

    Science.gov (United States)

    Danilov, Anton; Shaposhnikov, Mikhail; Plyusnina, Ekaterina; Kogan, Valeria; Fedichev, Peter; Moskalev, Alexey

    2013-09-01

    Mutations of the PI3K, TOR, iNOS, and NF-κB genes increase lifespan of model organisms and reduce the risk of some aging-associated diseases. We studied the effects of inhibitors of PI3K (wortmannin), TOR (rapamycin), iNOS (1400W), NF-κB (pyrrolidin dithiocarbamate and QNZ), and the combined effects of inhibitors: PI3K (wortmannin) and TOR (rapamycin), NF-κB (pyrrolidin dithiocarbamates) and PI3K (wortmannin), NF-κB (pyrrolidine dithiocarbamates) and TOR (rapamycin) on Drosophila melanogaster lifespan and quality of life (locomotor activity and fertility). Our data demonstrate that pharmacological inhibition of PI3K, TOR, NF-κB, and iNOS increases lifespan of Drosophila without decreasing quality of life. The greatest lifespan expanding effect was achieved by a combination of rapamycin (5 μM) and wortmannin (5 μM) (by 23.4%). The bioinformatic analysis (KEGG, REACTOME.PATH, DOLite, and GO.BP) showed the greatest aging-suppressor activity of rapamycin, consistent with experimental data. PMID:24096697

  16. Quantification of food intake in Drosophila.

    Directory of Open Access Journals (Sweden)

    Richard Wong

    Full Text Available Measurement of food intake in the fruit fly Drosophila melanogaster is often necessary for studies of behaviour, nutrition and drug administration. There is no reliable and agreed method for measuring food intake of flies in undisturbed, steady state, and normal culture conditions. We report such a method, based on measurement of feeding frequency by proboscis-extension, validated by short-term measurements of food dye intake. We used the method to demonstrate that (a female flies feed more frequently than males, (b flies feed more often when housed in larger groups and (c fly feeding varies at different times of the day. We also show that alterations in food intake are not induced by dietary restriction or by a null mutation of the fly insulin receptor substrate chico. In contrast, mutation of takeout increases food intake by increasing feeding frequency while mutation of ovo(D increases food intake by increasing the volume of food consumed per proboscis-extension. This approach provides a practical and reliable method for quantification of food intake in Drosophila under normal, undisturbed culture conditions.

  17. Fascin regulates nuclear actin during Drosophila oogenesis.

    Science.gov (United States)

    Kelpsch, Daniel J; Groen, Christopher M; Fagan, Tiffany N; Sudhir, Sweta; Tootle, Tina L

    2016-10-01

    Drosophila oogenesis provides a developmental system with which to study nuclear actin. During Stages 5-9, nuclear actin levels are high in the oocyte and exhibit variation within the nurse cells. Cofilin and Profilin, which regulate the nuclear import and export of actin, also localize to the nuclei. Expression of GFP-tagged Actin results in nuclear actin rod formation. These findings indicate that nuclear actin must be tightly regulated during oogenesis. One factor mediating this regulation is Fascin. Overexpression of Fascin enhances nuclear GFP-Actin rod formation, and Fascin colocalizes with the rods. Loss of Fascin reduces, whereas overexpression of Fascin increases, the frequency of nurse cells with high levels of nuclear actin, but neither alters the overall nuclear level of actin within the ovary. These data suggest that Fascin regulates the ability of specific cells to accumulate nuclear actin. Evidence indicates that Fascin positively regulates nuclear actin through Cofilin. Loss of Fascin results in decreased nuclear Cofilin. In addition, Fascin and Cofilin genetically interact, as double heterozygotes exhibit a reduction in the number of nurse cells with high nuclear actin levels. These findings are likely applicable beyond Drosophila follicle development, as the localization and functions of Fascin and the mechanisms regulating nuclear actin are widely conserved.

  18. Lamin C and chromatin organization in Drosophila

    Indian Academy of Sciences (India)

    B. V. Gurudatta; L. S. Shashidhara; Veena K. Parnaik

    2010-04-01

    Drosophila lamin C (LamC) is a developmentally regulated component of the nuclear lamina. The lamC gene is situated in the fifth intron of the essential gene tout velu (ttv). We carried out genetic analysis of lamC during development. Phenotypic analyses of RNAi-mediated downregulation of lamC expression as well as targeted misexpression of lamin C suggest a role for lamC in cell survival. Of particular interest in the context of laminopathies is the caspase-dependent apoptosis induced by the overexpression of lamin C. Interestingly, misexpression of lamin C in the central nervous system, where it is not normally expressed, did not affect organization of the nuclear lamina. lamC mutant alleles suppressed position effect variegation normally displayed at near-centromeric and telomeric regions. Further, both downregulation and misexpression of lamin C affected the distribution of heterochromatin protein 1. Our results suggest that Drosophila lamC has a tissue-specific role during development and is required for chromatin organization.

  19. Egg-laying rhythm in Drosophila melanogaster

    Indian Academy of Sciences (India)

    T. Manjunatha; Shantala Hari Dass; Vijay Kumar Sharma

    2008-12-01

    Extensive research has been carried out to understand how circadian clocks regulate various physiological processes in organisms. The discovery of clock genes and the molecular clockwork has helped researchers to understand the possible role of these genes in regulating various metabolic processes. In Drosophila melanogaster, many studies have shown that the basic architecture of circadian clocks is multi-oscillatory. In nature, different neuronal subgroups in the brain of D. melanogaster have been demonstrated to control different circadian behavioural rhythms or different aspects of the same circadian rhythm. Among the circadian phenomena that have been studied so far in Drosophila, the egg-laying rhythm is unique, and relatively less explored. Unlike most other circadian rhythms, the egg-laying rhythm is rhythmic under constant light conditions, and the endogenous or free-running period of the rhythm is greater than those of most other rhythms. Although the clock genes and neurons required for the persistence of adult emergence and activity/rest rhythms have been studied extensively, those underlying the circadian egg-laying rhythm still remain largely unknown. In this review, we discuss our current understanding of the circadian egg-laying rhythm in D. melanogaster, and the possible molecular and physiological mechanisms that control the rhythmic output of the egg-laying process.

  20. Drosophila roadblock and Chlamydomonas Lc7

    Science.gov (United States)

    Bowman, Aaron B.; Patel-King, Ramila S.; Benashski, Sharon E.; McCaffery, J. Michael; Goldstein, Lawrence S.B.; King, Stephen M.

    1999-01-01

    Eukaryotic organisms utilize microtubule-dependent motors of the kinesin and dynein superfamilies to generate intracellular movement. To identify new genes involved in the regulation of axonal transport in Drosophila melanogaster, we undertook a screen based upon the sluggish larval phenotype of known motor mutants. One of the mutants identified in this screen, roadblock (robl), exhibits diverse defects in intracellular transport including axonal transport and mitosis. These defects include intra-axonal accumulations of cargoes, severe axonal degeneration, and aberrant chromosome segregation. The gene identified by robl encodes a 97–amino acid polypeptide that is 57% identical (70% similar) to the 105–amino acid Chlamydomonas outer arm dynein–associated protein LC7, also reported here. Both robl and LC7 have homology to several other genes from fruit fly, nematode, and mammals, but not Saccharomyces cerevisiae. Furthermore, we demonstrate that members of this family of proteins are associated with both flagellar outer arm dynein and Drosophila and rat brain cytoplasmic dynein. We propose that roadblock/LC7 family members may modulate specific dynein functions. PMID:10402468

  1. The complexity of Drosophila innate immunity

    Directory of Open Access Journals (Sweden)

    A Reumer

    2010-01-01

    Full Text Available Metazoans rely on efficient mechanisms to oppose infections caused by pathogens. The immediate and first-line defense mechanism(s in metazoans, referred to as the innate immune system, is initiated upon recognition of microbial intruders by germline encoded receptors and is executed by a set of rapid effector mechanisms. Adaptive immunity is restricted to vertebrate species and it is controlled and assisted by the innate immune system.Interestingly, most of the basic signaling cascades that regulate the primeval innate defense mechanism(s have been well conserved during evolution, for instance between humans and the fruit fly, Drosophila melanogaster. Being devoid of adaptive signaling and effector systems, Drosophila has become an established model system for studying pristine innate immune cascades and reactions. In general, an immune response is evoked when microorganisms pass the fruit fly’s physical barriers (e.g., cuticle, epithelial lining of gut and trachea, and it is mainly executed in the hemolymph, the equivalent of the mammalian blood. Innate immunity in the fruit fly consists of a phenoloxidase (PO response, a cellular response (hemocytes, an antiviral response, and the NF-κB dependent production of antimicrobial peptides referred to as the humoral response. The JAK/STAT and Jun kinase signaling cascades are also implicated in the defence against pathogens.

  2. Understanding Consumer Preferences for Australian Sparkling Wine vs. French Champagne

    Directory of Open Access Journals (Sweden)

    Julie Culbert

    2016-07-01

    Full Text Available Sparkling wine represents a small but significant proportion of the Australian wine industry’s total production. Yet, Australia remains a significant importer of French Champagne. This study investigated consumer preferences for Australian sparkling wine vs. French Champagne and any compositional and/or sensorial bases for these preferences. A range of French and Australian sparkling wines were analyzed by MIR spectroscopy to determine if sparkling wines could be differentiated according to country of origin. A subset of wines, comprising two French Champagnes, a French sparkling wine and three Australian sparkling wines, were selected for (i descriptive analysis to characterize their sensory profiles and (ii acceptance tests to determine consumer liking (n = 95 Australian wine consumers. Significant differences were observed between liking scores; on average, the $70 French Champagne was liked least and the $12 Australian sparkling wine liked most, but segmentation (based on individual liking scores identified clusters comprising consumers with distinct wine preferences. Interestingly, when consumers were shown wine bottle labels, they considered French wines to be more expensive than Australian wines, demonstrating a clear country of origin influence.

  3. Slovenia as a locale in contemporary Australian verse

    Directory of Open Access Journals (Sweden)

    Igor Maver

    1997-12-01

    Full Text Available Despite the fact that the writer Patrick White had worked on his novels for a short while also at Lake Bled in Slovenia at Hotel "Toplice", just like Agatha Christie did at Lake Bohinj, Slovenia has only recently come to feature in mainstream Australian literature, more precisely in contemporary Australian poetry. It should be stressed that Slovenia is thus no longer present only in Slovene migrant poetry written in Australia as has so far been the case: it entered the major contemporary Australian anthologies. This testifies to the fact that Slovenia no longer belongs to the uncharted part of Central Europe on the geographical and consequently also on the Australian literary map. Rather than that Slovenia increasingly makes part of an average Australian 'Grand Tour' travel itinerary in Europe; it has thus become present in the Australian cultural consciousness. In this light two recent Australian poems with Slovenia as a literary locale are discussed, Andrew Taylor's "Morning in Ljubljana" I and Susan Hampton's poem "Yugoslav Story".

  4. Occupational exposure to radon in Australian Tourist Caves an Australian-wide study of radon levels

    Energy Technology Data Exchange (ETDEWEB)

    Solomon, S.B.; Langroo, R.; Peggie, J.R. [Australian Radiation Laboratory. Yallambie, VIC (Australia); Lyons, R.G. [University of Auckland, Auckland, (New Zealand). Department of Physics; James, J.M. [University of Sydney, Sydney, NSW (Australia). Department of Chemisty

    1996-02-01

    The study described in this report sets out to determine which Australian show caves have long- term radon levels in excess of the proposed action level of 1000 Bq m{sup -3}. The collaborative study between the Australian Radiation Laboratory (ARL), the University of Sydney and the University of Auckland, was carried out with the support of a Research Grant from Worksafe Australia. The aims of this study were to measure radon levels for each season over a period of one year, at representative sites in all developed show caves around Australia, to determine yearly average radon levels for each cave tour, based on these site measurements, to estimate the radiation doses to the tour guides employed in these caves, and to identify caves with radon concentrations in excess of the action level. (authors) 7 refs., 10 tabs., 2 figs.

  5. Occupational exposure to radon in Australian Tourist Caves an Australian-wide study of radon levels

    International Nuclear Information System (INIS)

    The study described in this report sets out to determine which Australian show caves have long- term radon levels in excess of the proposed action level of 1000 Bq m-3. The collaborative study between the Australian Radiation Laboratory (ARL), the University of Sydney and the University of Auckland, was carried out with the support of a Research Grant from Worksafe Australia. The aims of this study were to measure radon levels for each season over a period of one year, at representative sites in all developed show caves around Australia, to determine yearly average radon levels for each cave tour, based on these site measurements, to estimate the radiation doses to the tour guides employed in these caves, and to identify caves with radon concentrations in excess of the action level. (authors)

  6. Technologies, Democracy and Digital Citizenship: Examining Australian Policy Intersections and the Implications for School Leadership

    OpenAIRE

    Kathryn Moyle

    2014-01-01

    There are intersections that can occur between the respective peak Australian school education policy agendas. These policies include the use of technologies in classrooms to improve teaching and learning as promoted through the Melbourne Declaration on Educational Goals for Young Australians and the Australian Curriculum; and the implementation of professional standards as outlined in the Australian Professional Standard for Principals and the Australian Professional Standards for Teachers. ...

  7. Beverage intake and obesity in Australian children

    Directory of Open Access Journals (Sweden)

    Clifton Peter M

    2011-12-01

    Full Text Available Abstract Background There have been increases in the obesity and overweight rates in Australian children over the past 25 years and it has been suggested that sugar sweetened beverages (SSB have played a role in this increase. Objective The objectives of this study were to: (1 examine SSB intakes in the 2007 Australian Children's Nutrition and Physical Activity Survey (2 relate SSB intake to rates of overweight and obesity, socio-economic status (SES, TV viewing time, and activity levels and (3 compare 2007 SSB intakes with data from the 1995 National Nutrition Survey. Design A computer assisted 24 h dietary recall in 4,400 children aged 2-16 years was performed. Results In the 2007 survey 47% of all children reported drinking SSBs with 25% consuming sugar sweetened soft drinks on the day of the survey. The mean consumption of soft drink was 436 g/d/consumer. Activity levels were unrelated to SSB consumption. Television viewing was positively related to soft drink consumption with a difference of 55 g/day from bottom to top tertile of time spent TV viewing (p = 0.015 in children aged 9-16 years. 55% of SSB consumption occurred at home and 10% occurred at school. Lower SES status was associated with a greater prevalence of SSB consumption- 30% for the lowest SES quartile vs 19% in the highest quartile. The proportion of overweight who consumed SSBs (which excludes 100% fruit was not different from the non-overweight children although the proportion of SSB consumers in the 6% of children who were obese was significant compared with the non-overweight children (59% vs 47%, p Conclusions This cross-sectional data set provides evidence that SSB consumption for Australian children is still high despite the decrease since 1995 in some age groups. It provides little support to conclude that overweight in children is currently being driven by excessive SSB consumption although it may be factor in some obese children. Conclusions are limited by the cross

  8. An Empirical Analysis of Australian Gold Mining Firms

    OpenAIRE

    Baur, Dirk G.

    2012-01-01

    This paper studies the exposure of Australian gold-mining firms to changes in the gold price, the stock market and the Australian dollar - US dollar exchange rate. The empirical analysis uses daily, weekly and monthly data of all gold-mining firms in the S&P/ASX All Ordinaries Gold Index for the period from January 1980 to December 2010 and finds that the average gold beta is 0.67 for gold denominated in US dollar and 0.38 for gold denominated in Australian dollars. The study also finds subst...

  9. The dominant mutation Suppressor of black indicates that de novo pyrimindine biosynthesis is involved in the Drosophila tan pigmentation pathway

    DEFF Research Database (Denmark)

    Piskur, Jure; Kolbak, D.; Søndergaard, Leif;

    1993-01-01

    Pyrimidines, beta-alanine, cuticle, drosophila, pyrimidine analogs, molecular genetics, rudimentary......Pyrimidines, beta-alanine, cuticle, drosophila, pyrimidine analogs, molecular genetics, rudimentary...

  10. Functional Gustatory Role of Chemoreceptors in Drosophila Wings.

    Science.gov (United States)

    Raad, Hussein; Ferveur, Jean-François; Ledger, Neil; Capovilla, Maria; Robichon, Alain

    2016-05-17

    Neuroanatomical evidence argues for the presence of taste sensilla in Drosophila wings; however, the taste physiology of insect wings remains hypothetical, and a comprehensive link to mechanical functions, such as flight, wing flapping, and grooming, is lacking. Our data show that the sensilla of the Drosophila anterior wing margin respond to both sweet and bitter molecules through an increase in cytosolic Ca(2+) levels. Conversely, genetically modified flies presenting a wing-specific reduction in chemosensory cells show severe defects in both wing taste signaling and the exploratory guidance associated with chemodetection. In Drosophila, the chemodetection machinery includes mechanical grooming, which facilitates the contact between tastants and wing chemoreceptors, and the vibrations of flapping wings that nebulize volatile molecules as carboxylic acids. Together, these data demonstrate that the Drosophila wing chemosensory sensilla are a functional taste organ and that they may have a role in the exploration of ecological niches. PMID:27160896

  11. Functional Gustatory Role of Chemoreceptors in Drosophila Wings.

    Science.gov (United States)

    Raad, Hussein; Ferveur, Jean-François; Ledger, Neil; Capovilla, Maria; Robichon, Alain

    2016-05-17

    Neuroanatomical evidence argues for the presence of taste sensilla in Drosophila wings; however, the taste physiology of insect wings remains hypothetical, and a comprehensive link to mechanical functions, such as flight, wing flapping, and grooming, is lacking. Our data show that the sensilla of the Drosophila anterior wing margin respond to both sweet and bitter molecules through an increase in cytosolic Ca(2+) levels. Conversely, genetically modified flies presenting a wing-specific reduction in chemosensory cells show severe defects in both wing taste signaling and the exploratory guidance associated with chemodetection. In Drosophila, the chemodetection machinery includes mechanical grooming, which facilitates the contact between tastants and wing chemoreceptors, and the vibrations of flapping wings that nebulize volatile molecules as carboxylic acids. Together, these data demonstrate that the Drosophila wing chemosensory sensilla are a functional taste organ and that they may have a role in the exploration of ecological niches.

  12. Correction: Serendipitous discovery of Wolbachia genomes in multiple Drosophila species

    OpenAIRE

    Salzberg, Steven L.; Julie C Dunning Hotopp; Delcher, Arthur L; Pop, Mihai; Smith, Douglas R; Eisen, Michael B; Nelson, William C.

    2005-01-01

    A correction to Serendipitous discovery of Wolbachia genomes in multiple Drosophila species by SL Salzberg, JC Dunning Hotopp, AL Delcher, M Pop, DR Smith, MB Eisen and WC Nelson. Genome Biology 2005, 6:R23

  13. Is premating isolation in Drosophila overestimated due to uncontrolled factors?

    Indian Academy of Sciences (India)

    Pelayo Casares; Rafael Piñeiro; Maria C. Carracedo

    2005-12-01

    Sexual isolation in Drosophila is typically measured by multiple-choice mating tests. While many environmental variables during such tests are controlled by the researcher, there are some factors that are usually uncontrolled. We demonstrate, using Drosophila melanogaster and D. pseudoobscura flies, that the temperature of rearing, preadult density, and level of consanguinity, can all produce differences in mating propensity between genetically equivalent flies. These differences in mating propensity, in turn, can give rise to statistically significant results in multiple-choice mating tests, leading to positive isolation values and the artifactual inference of sexual isolation between populations. This fact agrees with a nonrandom excess of significant positive tests found in a review of the literature of Drosophila intraspecific mating choice. An overestimate of true cases of sexual isolation in Drosophila in the literature can, therefore, not be ruled out.

  14. Molecular evolution of a Drosophila homolog of human BRCA2.

    Science.gov (United States)

    Bennett, Sarah M; Noor, Mohamed A F

    2009-11-01

    The human cancer susceptibility gene, BRCA2, functions in double-strand break repair by homologous recombination, and it appears to function via interaction of a repetitive region ("BRC repeats") with RAD-51. A putatively simpler homolog, dmbrca2, was identified in Drosophila melanogaster recently and also affects mitotic and meiotic double-strand break repair. In this study, we examined patterns of repeat variation both within Drosophila pseudoobscura and among available Drosophila genome sequences. We identified extensive variation within and among closely related Drosophila species in BRC repeat number, to the extent that variation within this genus recapitulates the extent of variation found across the entire animal kingdom. We describe patterns of evolution across species by documenting recent repeat expansions (sometimes in tandem arrays) and homogenizations within available genome sequences. Overall, we have documented patterns and modes of evolution in a new model system of a gene which is important to human health.

  15. Western Australian school students' understanding of biotechnology

    Science.gov (United States)

    Dawson, Vaille; Schibeci, Renato

    2003-01-01

    Are science educators providing secondary school students with the background to understand the science behind recent controversies such as the recently introduced compulsory labelling of genetically modified foods? Research from the UK suggests that many secondary school students do not understand the processes or implications of modern biotechnology. The situation in Australia is unclear. In this study, 1116 15-year-old students from eleven Western Australian schools were surveyed to determine their understanding of, and attitude towards, recent advances in modern biotechnology. The results indicate that approximately one third of students have little or no understanding of biotechnology. Many students over-estimate the use of biotechnology in our society by confusing current uses with possible future applications. The results provide a rationale for the inclusion of biotechnology, a cutting edge science, in the school science curriculum

  16. The Australian RSI debate: stereotyping and medicine.

    Science.gov (United States)

    Quintner, J L

    1995-07-01

    The vehement scientific debate which took place in Australia in the 1980s over the epidemic of the chronic cervicobrachial pain syndrome known as repetition strain injury (RSI) was remarkable for the accompanying social commentary offered by many of the medical participants. This commentary was to have a profound effect on relationships between individual doctors and their patients with RSI. It reflected and reinforced the prevailing stereotypes within Australian society, not only of working women, but also of recipients of workers' compensation payments. On the other hand, some of the medical responses to the epidemic were severely criticized by social scientists who analysed the epidemic. In the process of such criticism, a number of stereotypes of doctors were also reinforced. PMID:7626774

  17. Sustainability and Competitiveness in Australian Cities

    Directory of Open Access Journals (Sweden)

    Richard Hu

    2015-02-01

    Full Text Available This study injects sustainability into competitiveness to inform policy making and planning for contemporary urban development. This is built upon the recent advancement in the scholarship on urban competitiveness that demonstrates a clear deviation from an economic-centric approach to incorporate multiple dimensions of a city’s progress. This study has an explicit concern for environmental sustainability and its relationship with urban competitiveness and their conceptual and methodological articulations. Empirically, this study measures the sustainability and competitiveness in Australian cities and reveals that Australia’s urban progress is clearly associated with an environmental cost. The findings are useful to inform policy making and planning for building sustainable and competitive cities. Apart from the conventional solutions that focus on urban form change and transport infrastructure improvement, this study suggests a need to explore the opportunities deriving from the emerging smart city planning and practice.

  18. "Bridging the Gap" through Australian Cultural Astronomy

    CERN Document Server

    Hamacher, Duane W

    2011-01-01

    For more than 50,000 years, Indigenous Australians have incorporated celestial events into their oral traditions and used the motions of celestial bodies for navigation, time-keeping, food economics, and social structure. In this paper, we explore the ways in which Aboriginal people made careful observations of the sky, measurements of celestial bodies, and incorporated astronomical events into complex oral traditions by searching for written records of time-keeping using celestial bodies, the use of rising and setting stars as indicators of special events, recorded observations of variable stars, the solar cycle, and lunar phases (including ocean tides and eclipses) in oral tradition, as well as astronomical measurements of the equinox, solstice, and cardinal points.

  19. Australian Coral as a Biomaterial: Characteristics

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    In order to produce effective implants, the materials used must be biocompatible. Hydroxyapatite (HAp) is a bioactive material similar to the mineral component of teeth and bone which is often used for orbital implants and bone graft applications. HAp can be manufactured from corals via hydrothermal conversion. Coral is particularly useful as a starting material for hydroxyapatite production because of its porous nature. When a porous structure is used tissue ingrowth can occur readily and hence an excellent mechanical bond can be achieved. A large pore size and a high degree of pore interconnections are desirable implant properties. In the present paper a comparison of the properties of four different species of Australian coral has been made to determine the most favourable species to use as a starting material for hydrothermal conversion.

  20. Bazooka mediates secondary axon morphology in Drosophila brain lineages

    OpenAIRE

    Hartenstein Volker; Spindler Shana R

    2011-01-01

    Abstract In the Drosophila brain, neural lineages project bundled axon tracts into a central neuropile. Each lineage exhibits a stereotypical branching pattern and trajectory, which distinguish it from other lineages. In this study, we used a multilineage approach to explore the neural function of the Par-complex member Par3/Bazooka in vivo. Drosophila bazooka is expressed in post-mitotic neurons of the larval brain and localizes within neurons in a lineage-dependent manner. The fact that mul...

  1. A development-based compartmentalization of the Drosophila central brain

    OpenAIRE

    Pereanu, Wayne; Kumar, Abilasha; Jennett, Arnim; Reichert, Heinrich; Hartenstein, Volker

    2010-01-01

    The neuropile of the Drosophila brain is subdivided into anatomically discrete compartments. Compartments are rich in terminal neurite branching and synapses; they are the neuropile domains in which signal processing takes place. Compartment boundaries are defined by more or less dense layers of glial cells, as well as long neurite fascicles. These fascicles are formed during the larval period when the approximately 100 neuronal lineages that constitute the Drosophila central brain differenti...

  2. Functional gustatory role of chemoreceptors in drosophila wings

    OpenAIRE

    Raad, Hussein; Ferveur, Jean-François; Ledger, Neil; Capovilla, Maria

    2016-01-01

    Neuroanatomical evidence argues for the presence of taste sensilla in Drosophila wings; however, the taste physiology of insect wings remains hypothetical, and a comprehensive link to mechanical functions, such as flight, wing flapping, and grooming, is lacking. Our data show that the sensilla of the Drosophila anterior wing margin respond to both sweet and bitter molecules through an increase in cytosolic Ca2+ levels. Conversely, genetically modified flies presenting a wing-specific reductio...

  3. Circadian Organization of Behavior and Physiology in Drosophila

    OpenAIRE

    Allada, Ravi; Chung, Brian Y.

    2010-01-01

    Circadian clocks organize behavior and physiology to adapt to daily environmental cycles. Genetic approaches in the fruit fly, Drosophila melanogaster, have revealed widely conserved molecular gears of these 24-h timers. Yet much less is known about how these cell-autonomous clocks confer temporal information to modulate cellular functions. Here we discuss our current knowledge of circadian clock function in Drosophila, providing an overview of the molecular underpinnings of circadian clocks....

  4. FlyBase: a Drosophila database. Flybase Consortium.

    OpenAIRE

    1998-01-01

    FlyBase (http://flybase.bio.indiana.edu/) is a comprehensive database of genetic and molecular data concerning Drosophila . FlyBase is maintained as a relational database (in Sybase) and is made available as html documents and flat files. The scope of FlyBase includes: genes, alleles (with phenotypes), aberrations, transposons, pointers to sequence data, gene products, maps, clones, stock lists, Drosophila workers and bibliographic references.

  5. Dietary glucose regulates yeast consumption in adult Drosophila males

    OpenAIRE

    Sebastien eLebreton; Peter eWitzgall; Marie eOlsson; Becher, Paul G.

    2014-01-01

    The adjustment of feeding behavior in response to hunger and satiety contributes to homeostatic regulation in animals. The fruit fly Drosophila melanogaster feeds on yeasts growing on overripe fruit, providing nutrients required for adult survival, reproduction and larval growth. Here, we present data on how the nutritional value of food affects subsequent yeast consumption in Drosophila adult males. After a period of starvation, flies showed intensive yeast consumption. In comparison, flies ...

  6. Candidate Glutamatergic Neurons in the Visual System of Drosophila

    OpenAIRE

    Shamprasad Varija Raghu; Alexander Borst

    2011-01-01

    The visual system of Drosophila contains approximately 60,000 neurons that are organized in parallel, retinotopically arranged columns. A large number of these neurons have been characterized in great anatomical detail. However, studies providing direct evidence for synaptic signaling and the neurotransmitter used by individual neurons are relatively sparse. Here we present a first layout of neurons in the Drosophila visual system that likely release glutamate as their major neurotransmitter....

  7. Evolution of Drosophila ribosomal protein gene core promoters

    OpenAIRE

    Ma, Xiaotu; Zhang, Kangyu; Li, Xiaoman

    2008-01-01

    The coordinated expression of ribosomal protein genes (RPGs) has been well documented in many species. Previous analyses of RPG promoters focus only on Fungi and mammals. Recognizing this gap and using a comparative genomics approach, we utilize a motif-finding algorithm that incorporates cross-species conservation to identify several significant motifs in Drosophila RPG promoters. As a result, significant differences of the enriched motifs in RPG promoter are found among Drosophila, Fungi, a...

  8. Following the Motion of Polycomb Bodies in Living Drosophila Embryos.

    Science.gov (United States)

    Cheutin, Thierry; Cavalli, Giacomo

    2016-01-01

    During the last two decades, observation of cell nuclei in live microscopy evidences motion of nuclear compartments. Drosophila embryos constitute a good model to study nuclear dynamic during cell differentiation because they can easily be observed in live microscopy. Inside the cell nucleus, Polycomb group proteins accumulate in foci named Pc bodies. Here, we describe a method to visualize and analyze the motion of these nuclear compartments inside cell nuclei of Drosophila embryos. PMID:27659993

  9. Genomic and karyotypic variation in Drosophila parasitoids (Hymenoptera, Cynipoidea, Figitidae

    Directory of Open Access Journals (Sweden)

    Vladimir Gokhman

    2011-08-01

    Full Text Available Drosophila melanogaster Meigen, 1830 has served as a model insect for over a century. Sequencing of the 11 additional Drosophila Fallen, 1823 species marks substantial progress in comparative genomics of this genus. By comparison, practically nothing is known about the genome size or genome sequences of parasitic wasps of Drosophila. Here, we present the first comparative analysis of genome size and karyotype structures of Drosophila parasitoids of the Leptopilina Förster, 1869 and Ganaspis Förster, 1869 species. The gametic genome size of Ganaspis xanthopoda (Ashmead, 1896 is larger than those of the three Leptopilina species studied. The genome sizes of all parasitic wasps studied here are also larger than those known for all Drosophila species. Surprisingly, genome sizes of these Drosophila parasitoids exceed the average value known for all previously studied Hymenoptera. The haploid chromosome number of both Leptopilina heterotoma (Thomson, 1862 and L. victoriae Nordlander, 1980 is ten. A chromosomal fusion appears to have produced a distinct karyotype for L. boulardi (Barbotin, Carton et Keiner-Pillault, 1979 (n = 9, whose genome size is smaller than that of wasps of the L. heterotoma clade. Like L. boulardi, the haploid chromosome number for G. xanthopoda is also nine. Our studies reveal a positive, but non linear, correlation between the genome size and total chromosome length in Drosophila parasitoids. These Drosophila parasitoids differ widely in their host range, and utilize different infection strategies to overcome host defense. Their comparative genomics, in relation to their exceptionally well-characterized hosts, will prove to be valuable for understanding the molecular basis of the host-parasite arms race and how such mechanisms shape the genetic structures of insect communities.

  10. Commercialization of Australian advanced infrared technology

    Science.gov (United States)

    Redpath, John; Brown, Allen; Woods, William F.

    1995-09-01

    For several decades, the main thrust in infrared technology developments in Australia has been in two main sensor technologies: uncooled silicon chip printed bolometric sensors pioneered by DSTO's Kevin Liddiard, and precision engineered high quality Cadmium Mercury Telluride developed at DSTO under the guidance of Dr. Richard Hartley. In late 1993 a low cost infrared imaging device was developed at DSTO as a sensor for guided missiles. The combination of these three innovations made up a unique package that enabled Australian industry to break through the barriers of commercializing infrared technology. The privately owned company, R.J. Optronics Pty Ltd undertook the process of re-engineering a selection of these DSTO developments to be applicable to a wide range of infrared products. The first project was a novel infrared imager based on a Palmer scan (translated circle) mechanism. This device applies a spinning wedge and a single detector, it uses a video processor to convert the image into a standard rectangular format. Originally developed as an imaging seeker for a stand-off weapon, it is producing such high quality images at such a low cost that it is now also being adapted for a wide variety of other military and commercial applications. A technique for electronically stabilizing it has been developed which uses the inertial signals from co-mounted sensors to compensate for platform motions. This enables it to meet the requirements of aircraft, marine vessels and masthead sight applications without the use of gimbals. After tests on a three-axis motion table, several system configurations have now been successfully operated on a number of lightweight platforms, including a Cessna 172 and the Australian made Seabird Seeker aircraft.

  11. Australian Meningococcal Surveillance Programme annual report, 2013.

    Science.gov (United States)

    Lahra, Monica M; Enriquez, Rodney P

    2014-12-31

    In 2013, there were 143 laboratory-confirmed cases of invasive meningococcal disease (IMD) analysed by the Australian National Neisseria Network (NNN). This was the lowest number of laboratory confirmed IMD cases referred to the NNN since the inception of the Australian Meningococcal Surveillance Programme in 1994. Probable and laboratory confirmed IMD is notifiable in Australia. There were 149 IMD cases notified to the National Notifiable Diseases Surveillance System in 2013. Meningococcal serogrouping was determined for 139/143 laboratory confirmed IMD cases; 74.8% (104 cases) were serogroup B infections; 5.8% (8 cases) were serogroup C infections; 8.6% (12 cases) were serogroup W135; and 10.8% (15 cases) were serogroup Y. Primary and secondary disease peaks were observed, respectively, in those aged 4 years or less, and in adolescents (15-19 years). Serogroup B cases predominated in all jurisdictions and age groups, except for those aged 65 years or over where serogroup Y predominated. The overall proportion and number of IMD caused by serogroup B decreased from previous years. The number of cases of IMD caused by serogroup C was low, and has been proportionally stable over recent years. The number of IMD cases caused by W135 and Y serogroups was similar to previous years but the proportion has increased with the overall reduction in numbers of IMD cases. Molecular typing was performed on 92 of the 93 IMD isolates, and 23 of the 50 cases confirmed by nucleic acid amplification testing. In 2013, the most common porA genotype circulating in Australia was P1.7-2,4. All IMD isolates tested were susceptible to ceftriaxone; ciprofloxacin and rifampicin. Decreased susceptibility to penicillin was observed in 78.5% of isolates.

  12. The Performance of Western Australian Ports

    Institute of Scientific and Technical Information of China (English)

    Malcolm Tull; Fred Affleck

    2008-01-01

    <正>The aim of this paper is to undertake an analysis of the performance of Western Australia’s port authorities.The context for this research is the report released in February 2006 by Access Economics (A scorecard of the design of economic regulation of infrastructure) for the Australian Council for Infrastructure Development.This report was critical of the regime for economic regulation of Western Australia’s ports,and by implication of the potential quality and efficiency of service delivery to their principal stakeholders.However,a reading of the Access Economics report and supporting data suggests that its analysis takes no account of the regulatory frameworks for port authorities in Western Austral ia(WA) contained in the Port Authorities Act 1999(WA) and elsewhere,or of the actual economic and physical performance of WA port authorities.In the light of this apparently flawed analysis of the effectiveness of port regulation in WA,it is timely to review the performance of ports under the current governance structures,and to place the Access Economics report in a broader empirical performance-based context. While there is no regime for direct regulation of access to WA’s port infrastructure,it is argued that provisions in WA’s legislation governing the management of ports provide much of the focus,transparency and accountability required of an adequate regulatory framework.The current dominant Australian model of public ownership,with ports acting as strategic managers subject to statutory and governmental oversight,offers a viable alternative to complete privatisation and specialised regulatory controls.Efficient ports arguably can emerge from a variety of institutional frameworks-there is no single ownership or administrative structure that fits all circumstances.

  13. Nuclear techniques in Australian animal production

    International Nuclear Information System (INIS)

    In tropical and sub-tropical regions, the production of domestic animals is frequently depressed by the climatic and ecological conditions. These negative effects can be overcome to a great extent by improved methods of animal and land management. In animal research, nuclear techniques are playing an important role in the study of different aspects of nutrition, metabolism, reproduction and health of domestic animals. In response to the need expressed by Member States for more information on these techniques, the Joint FAO/IAEA Division of Atomic Energy in Food and Agriculture and the IAEA's Division of Technical Assistance organized a study tour to Australia, a country which has developed considerable expertise in agricultural and animal research. The purpose of the study tour was to enable veterinary and animal scientists and administrators from developing countries in Asia and the Far East to observe at first hand the ways in which animal production, particularly meat, milk and wool, can be increased in tropical and sub-tropical areas. Fourteen senior scientists and research directors from seven Asian countries (Bangladesh, India, Republic of Korea, Malaysia, Philippines, Sri Lanka and Thailand) participated. The counterpart organizations in Australia were the Australian Development Assistance Agency (ADAA) and the Commonwealth Scientific and Industrial Research Organization (CSIRO). The chief programmer and co-ordinator of the study tour was John E. Vercoe, officer-in-charge of CSIRO's Tropical Cattle Research Centre in Rockhampton, and a former IAEA staff member. The tour was financed by the United Nations Development Programme. The participants visited research facilities of universities, national and state laboratories and commercial cattle producers. The tour started at Sydney and proceeded north along the east coast of Australia to Townsville. On the way, major stops were made in Armidale, Grafton, Wollongbar, Brisbane and Rockhampton. In Rockhampton, a

  14. Achieving professional status: Australian podiatrists' perceptions

    Directory of Open Access Journals (Sweden)

    Vernon Wesley

    2009-02-01

    Full Text Available Abstract Background This paper explores the notion of professional status from the perspective of a sample of Australian podiatrists; how it is experienced, what factors are felt to affect it, and how these are considered to influence professional standing within an evolving healthcare system. Underpinning sociological theory is deployed in order to inform and contextualise the study. Methods Data were drawn from a series of in-depth semi-structured interviews (n = 21 and focus groups (n = 9 with podiatrists from across four of Australia's eastern states (Queensland, New South Wales, Victoria and Australian Capital Territory, resulting in a total of 76 participants. Semi-structured interview schedules sought to explore podiatrist perspectives on a range of features related to professional status within podiatry in Australia. Results Central to the retention and enhancement of status was felt to be the development of specialist roles and the maintenance of control over key task domains. Key distinctions in private and public sector environments, and in rural and urban settings, were noted and found to reflect differing contexts for status development. Marketing was considered important to image enhancement, as was the cache attached to the status of the universities providing graduate education. Conclusion Perceived determinants of professional status broadly matched those identified in the wider sociological literature, most notably credentialism, client status, content and context of work (such as specialisation and an ideological basis for persuading audiences to acknowledge professional status. In an environment of demographic and workforce change, and the resultant policy demands for healthcare service re-design, enhanced opportunities for specialisation appear evident. Under the current model of professionalism, both role flexibility and uniqueness may prove important.

  15. The bacterial communities of Drosophila suzukii collected from undamaged cherries

    Directory of Open Access Journals (Sweden)

    James Angus Chandler

    2014-07-01

    Full Text Available Drosophila suzukii is an introduced pest insect that feeds on undamaged, attached fruit. This diet is distinct from the fallen, discomposing fruits utilized by most other species of Drosophila. Since the bacterial microbiota of Drosophila, and of many other animals, is affected by diet, we hypothesized that the bacteria associated with D. suzukii are distinct from that of other Drosophila. Using 16S rDNA PCR and Illumina sequencing, we characterized the bacterial communities of larval and adult D. suzukii collected from undamaged, attached cherries in California, USA. We find that the bacterial communities associated with these samples of D. suzukii contain a high frequency of Tatumella. Gluconobacter and Acetobacter, two taxa with known associations with Drosophila, were also found, although at lower frequency than Tatumella in four of the five samples examined. Sampling D. suzukii from different locations and/or while feeding on different fruits is needed to determine the generality of the results determined by these samples. Nevertheless this is, to our knowledge, the first study characterizing the bacterial communities of this ecologically unique and economically important species of Drosophila.

  16. Management Communication for the New Millennium: An Australian Perspective.

    Science.gov (United States)

    More, Elizabeth A.; Irwin, Harry T.

    2000-01-01

    Discusses management communications in the general area of identity, and the Australian situation involving management communication scholarship, management communication education, and the future of the field in Australia. (NH)

  17. The Australian cigarette brand as product, person, and symbol

    OpenAIRE

    Carter, S.

    2003-01-01

    Objective: To examine, for dominant Australian cigarette brands, brand identity (overriding brand vision), brand positioning (brand identity elements communicated to the consumer), brand image (consumers' brand perceptions) and brand equity (financial value).

  18. Heart failure among Indigenous Australians: a systematic review

    OpenAIRE

    Woods John A; Katzenellenbogen Judith M; Davidson Patricia M; Thompson Sandra C

    2012-01-01

    Abstract Background Cardiovascular diseases contribute substantially to the poor health and reduced life expectancy of Indigenous Australians. Heart failure is a common, disabling, progressive and costly complication of these disorders. The epidemiology of heart failure and the adequacy of relevant health service provision in Indigenous Australians are not well delineated. Methods A systematic search of the electronic databases PubMed, Embase, Web of Science, Cinahl Plus, Informit and Google ...

  19. Australian Power: Can renewable technologies change the dominant industry view?

    OpenAIRE

    Lynette Molyneaux; Craig Froome; Liam Wagner; John Foster

    2012-01-01

    With carbon dioxide the major contributor to anthropogenic climate change, being required to reduce the carbon emissions from burning coal for electricity presents a systemic shock to Australian power. The Australian government is committed to the development of its coal seam gas resources for export to lucrative world markets and to transition domestic power generation to greater resilience by moving away from a reliance on coal to lower-emissions intensive gas. Using a commercially availabl...

  20. Structural impediments to TQM in Australian health care.

    Science.gov (United States)

    Degeling, P; Carnegie, M

    1995-01-01

    The culture of quality called for by total quality management (TQM) has much to recommend it. Australian experience, however, suggests that it is not something that can easily be added to the profession-based structures and cultures prevailing in most Australian hospitals. Implementing TQM is not just a matter of advocating it. The institutional transformation implied by TQM requires additional action on multiple fronts, both internal and external to the hospital.

  1. Nucleonic gauges in the Australian mining and exploration industries

    International Nuclear Information System (INIS)

    On-line and in-situ nucleonic analysis systems have found widespread application in the Australian metalliferous mineral and coal industries. The rapid and reliable response of these systems has led to improved exploration and better control of mining and mineral processing. This paper reviews both types of nucleonic control system (on-line and in-situ) available in Australian exploration and mining market. (author)

  2. Going Places: Praxis and Pedagogy in Australian Cultural Studies

    OpenAIRE

    Rebecca Rey; Golnar Nabizadeh

    2011-01-01

    This article considers the pedagogical value of praxis in maintaining the relevance of cultural studies in the Australian academic environment. Following its highly politicised beginnings at the Birmingham Centre for Contemporary Cultural Studies, we consider whether traditional classroom practices are commensurate with the contemporary expectations of students and staff. As a working model of the current Australian university climate, we consider the discipline group of English and Cultural ...

  3. Raja Junankar, Economics of Immigration: Immigration and the Australian Economy

    OpenAIRE

    DOUGLAS, Kacey

    2016-01-01

    Abstract. The Economics of Immigration: Immigration and the Australian Economy is a compilation of academic articles written by P.N. Junankar and coauthors on the topic of immigration in Australia. From the effects of immigration on Australia’s economy to the Australian labor market environment immigrants encounter, this book addresses important questions regarding immigration that are relevant to any economy.Keywords. Australia, International migration, International economics.JEL. F00, F22,...

  4. How Big is the Speculative Component in Australian Share Prices?

    OpenAIRE

    Angela Black; Patricia Fraser; Nicolaas Groenewold

    2001-01-01

    Using 20 years of Australian quarterly data, this paper decomposes Australian share prices into their fundamental and speculative components. To do this we derive the fundamental share-price-output ratio and, hence, the fundamental share price from a resticted vector-autoregressive model relating the aggregate real share-price index to real output. Our estimates use different assumptions regarding shareholders required real rate of return. Our results imply that a significant speculative comp...

  5. Aboriginal Placenames : Naming and re-naming the Australian landscape

    OpenAIRE

    Hercus, Luise; Koch, Harold

    2009-01-01

    Aboriginal approaches to the naming of places across Australia differ radically from the official introduced Anglo-Australian system. However, many of these earlier names have been incorporated into contemporary nomenclature, with considerable reinterpretations of their function and form. Recently, state jurisdictions have encouraged the adoption of a greater number of Indigenous names, sometimes alongside the accepted Anglo-Australian terms, around Sydney Harbour, for example. In some cases,...

  6. The World's First and Newest Organic Magazines are Australian

    OpenAIRE

    Paull, John

    2009-01-01

    Australia was an early adopter and advocate for organic farming. The world's first farming organisation to adopt "organic" into its title was the Australian Organic Farming and Gardening Society (1944-1955). The Society published the Organic Farming DIgest, starting in April 1946. The Organic Farming Digest was the first "organic" agriculture journal in the world to be published by an association. The Australian Organic Farming and Gardening Society and the Organic Farming Digest both predat...

  7. "A deep fragrance of academia": the Australian Tobacco Research Foundation

    OpenAIRE

    Chapman, S; Carter, S.; Peters, M

    2003-01-01

    Objectives: (1) To review the history of the tobacco industry supported Australian Tobacco Research Foundation (ATRF)(1970–1994) for evidence of the industry's use of the Foundation to further its objectives that "more research was needed" on smoking and health and to promulgate the view that nicotine was not addictive. (2) To review efforts by public health advocates to discredit the ATRF as a public relations tool used by the Australian industry.

  8. Global Production Sharing in the Australian Automotive Trade

    OpenAIRE

    Kishor Sharma

    2012-01-01

    This paper contributes to the literature on global production sharing by investigating the experience of the Australian automotive industry, which has experienced significant structural change following trade liberalisation. Our analysis indicates that the globalisation of the world economy, together with developments in transport and communication, has significantly increased the importance of the global production network in the Australian automotive industry, leading to a substantial rise ...

  9. Changes in Dairy Food and Nutrient Intakes in Australian Adolescents

    OpenAIRE

    Therese A. O'Sullivan; Trevor A. Mori; Beilin, Lawrence J; Oddy, Wendy H; Carole E. Parker; Vivian, Wendy J.

    2012-01-01

    Dairy nutrients, such as calcium, are particularly important in adolescence, a critical time for growth and development. There are limited Australian data following individuals through adolescence, evaluating changes in dairy nutrient and dairy product consumption. We used a validated food frequency questionnaire to investigate consumption in adolescents participating in both the 14 and 17 year follow-ups of the Western Australian Pregnancy Cohort (Raine) Study. Most adolescents did not reach...

  10. Screening the dykes of Oz: lesbian representation on Australian television.

    Science.gov (United States)

    Beirne, Rebecca

    2009-01-01

    This article provides a survey of lesbian representation in Australian-made television programming. Beginning with the 1970s, when Australia led the Anglophone world in terms of queer representation on television, the article discusses major instances of queer women appearing on Australian television including in such shows as Prisoner, Home & Away, Neighbours, All Saints, The Secret Life of Us, Last Man Standing, and Kick. PMID:19197661

  11. Comparing Australian and US Corporate Default Risk using Quantile Regression

    OpenAIRE

    Allen, David E.; Akhmad R. Kramadibrata; Powell, R. J.; Abhay Kumar Singh

    2011-01-01

    The severe bank stresses of the Global Financial Crisis (GFC) have underlined the importance of understanding and measuring extreme credit risk. The Australian economy is widely considered to have fared much better than the US and most other major world economies. This paper applies quantile regression and Monte Carlo simulation to the Merton structural credit model to investigate the impact of extreme asset value fluctuations on default probabilities of Australian companies in comparison to ...

  12. Simulation of gene pyramiding in Drosophila melanogaster

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Gene pyramiding has been successfully practiced in plant breeding for developing new breeds or lines in which favorable genes from several different lines were integrated.But it has not been used in animal breeding,and some theoretical investigation and simulation analysis with respect to its strategies,feasibility and efficiency are needed before it can be implemented in animals.In this study,we used four different pure fines of Drosophila melanogaster,each of which is homozygous at a specific mutant gene with a visible effect on phenotype,to simulate the gene pyramiding process and analyze the duration and population size required in different pyramiding strategies.We finally got the ideal individuals,which are homozygous at the four target genes simultaneously.This study demonstrates that gene pyramiding is feasible in animal breeding and the interaction between genes may affect the final results.

  13. Innate immunity in Drosophila: Pathogens and pathways

    Institute of Scientific and Technical Information of China (English)

    Shubha Govind

    2008-01-01

    Following in the footsteps of traditional developmental genetics, research over the last 15 years has shown that innate immunity against bacteria and fungi is governed largely by two NF-κB signal transduction pathways, Toll and IMD. Antiviral immunity appears to stem from RNA interference, whereas resistance against parasitoids is conferred by Toll signaling. The identification of these post-transcriptional regulatory mechanisms and the annotation of most Drosophila immunity genes have derived from functional genomic studies using "model" pathogens, intact animals and cell lines. The D. melanogaster host has thus provided the core information that can be used to study responses to natural microbial and metazoan pathogens as they become identified, as well as to test ideas of selection and evolutionary change. These analyses are of general importance to understanding mechanisms of other insect host-pathogen interactions and determinants of variation in host resistance.

  14. A Protein Interaction Map of Drosophila melanogaster

    Science.gov (United States)

    Giot, L.; Bader, J. S.; Brouwer, C.; Chaudhuri, A.; Kuang, B.; Li, Y.; Hao, Y. L.; Ooi, C. E.; Godwin, B.; Vitols, E.; Vijayadamodar, G.; Pochart, P.; Machineni, H.; Welsh, M.; Kong, Y.; Zerhusen, B.; Malcolm, R.; Varrone, Z.; Collis, A.; Minto, M.; Burgess, S.; McDaniel, L.; Stimpson, E.; Spriggs, F.; Williams, J.; Neurath, K.; Ioime, N.; Agee, M.; Voss, E.; Furtak, K.; Renzulli, R.; Aanensen, N.; Carrolla, S.; Bickelhaupt, E.; Lazovatsky, Y.; DaSilva, A.; Zhong, J.; Stanyon, C. A.; Finley, R. L.; White, K. P.; Braverman, M.; Jarvie, T.; Gold, S.; Leach, M.; Knight, J.; Shimkets, R. A.; McKenna, M. P.; Chant, J.; Rothberg, J. M.

    2003-12-01

    Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans.

  15. Optogenetic Control of Gene Expression in Drosophila.

    Directory of Open Access Journals (Sweden)

    Yick-Bun Chan

    Full Text Available To study the molecular mechanism of complex biological systems, it is important to be able to artificially manipulate gene expression in desired target sites with high precision. Based on the light dependent binding of cryptochrome 2 and a cryptochrome interacting bHLH protein, we developed a split lexA transcriptional activation system for use in Drosophila that allows regulation of gene expression in vivo using blue light or two-photon excitation. We show that this system offers high spatiotemporal resolution by inducing gene expression in tissues at various developmental stages. In combination with two-photon excitation, gene expression can be manipulated at precise sites in embryos, potentially offering an important tool with which to examine developmental processes.

  16. Quantitative neuroanatomy for connectomics in Drosophila.

    Science.gov (United States)

    Schneider-Mizell, Casey M; Gerhard, Stephan; Longair, Mark; Kazimiers, Tom; Li, Feng; Zwart, Maarten F; Champion, Andrew; Midgley, Frank M; Fetter, Richard D; Saalfeld, Stephan; Cardona, Albert

    2016-01-01

    Neuronal circuit mapping using electron microscopy demands laborious proofreading or reconciliation of multiple independent reconstructions. Here, we describe new methods to apply quantitative arbor and network context to iteratively proofread and reconstruct circuits and create anatomically enriched wiring diagrams. We measured the morphological underpinnings of connectivity in new and existing reconstructions of Drosophila sensorimotor (larva) and visual (adult) systems. Synaptic inputs were preferentially located on numerous small, microtubule-free 'twigs' which branch off a single microtubule-containing 'backbone'. Omission of individual twigs accounted for 96% of errors. However, the synapses of highly connected neurons were distributed across multiple twigs. Thus, the robustness of a strong connection to detailed twig anatomy was associated with robustness to reconstruction error. By comparing iterative reconstruction to the consensus of multiple reconstructions, we show that our method overcomes the need for redundant effort through the discovery and application of relationships between cellular neuroanatomy and synaptic connectivity. PMID:26990779

  17. Gustatory processing and taste memory in Drosophila.

    Science.gov (United States)

    Masek, Pavel; Keene, Alex C

    2016-06-01

    Taste allows animals to discriminate the value and potential toxicity of food prior to ingestion. Many tastants elicit an innate attractive or avoidance response that is modifiable with nutritional state and prior experience. A powerful genetic tool kit, well-characterized gustatory system, and standardized behavioral assays make the fruit fly, Drosophila melanogaster, an excellent system for investigating taste processing and memory. Recent studies have used this system to identify the neural basis for acquired taste preference. These studies have revealed a role for dopamine-mediated plasticity of the mushroom bodies that modulate the threshold of response to appetitive tastants. The identification of neural circuitry regulating taste memory provides a system to study the genetic and physiological processes that govern plasticity within a defined memory circuit. PMID:27328844

  18. Antioxidants, metabolic rate and aging in Drosophila

    Science.gov (United States)

    Miquel, J.; Fleming, J.; Economos, A. C.

    1982-01-01

    The metabolic rate-of-living theory of aging was investigated by determining the effect of several life-prolonging antioxidants on the metabolic rate and life span of Drosophila. The respiration rate of groups of continuously agitated flies was determined in a Gilson respirometer. Vitamin E, 2,4-dinitrophenol, nordihydroguaiaretic acid, and thiazolidine carboxylic acid were employed as antioxidants. Results show that all of these antioxidants reduced the oxygen consumption rate and increased the mean life span, and a significant negative linear correlation was found between the mean life span and the metabolic rate. It is concluded that these findings indicate that some antioxidants may inhibit respiration rate in addition to their protective effect against free radical-induced cellular damage.

  19. Structure and Development of Glia in Drosophila

    Science.gov (United States)

    Hartenstein, Volker

    2014-01-01

    Insect glia represents a conspicuous and diverse population of cells and plays a role in controlling neuronal progenitor proliferation, axonal growth, neuronal differentiation and maintenance, and neuronal function. Genetic studies in Drosophila have elucidated many aspects of glial structure, function and development. Just as in vertebrates, it appears as if different classes of glial cells are specialized for different functions. Based on topology and cell shape, glial cells of the central nervous system fall into three classes (Fig. 1A–C): (i) surface glia that extend sheath-like processes to wrap around the entire brain; (ii) cortex glia (also called cell body-associated glia) that encapsulate neuronal somata and neuroblasts which form the outer layer (cortex) of the central nervous system; (iii) neuropile glia that are located at the interface between the cortex and the neuropile, the central domain of the nervous system formed by the highly branched neuronal processes and their synaptic contacts. Surface glia is further subdivided into an outer, perineurial layer, and an inner, subperineurial layer. Likewise, neuropile glia comprises a class of cells that remain at the surface of the neuropile (ensheathing glia), and a second class that forms profuse lamellar processes around nerve fibers within the neuropile (astrocyte-like or reticular glia). Glia also surrounds the peripheral nerves and sensory organs; here, one also recognizes perineurial and subperineurial glia, and a third type called “wrapping glia” that most likely corresponds to the ensheathing glia of the central nervous system. Much more experimental work is needed to determine how fundamental these differences between classes of glial cells are, or how and when during development they are specified. To aid in this work the following review will briefly summarize our knowledge of the classes of glial cells encountered in the Drosophila nervous system, and then survey their development from

  20. Isolation and characterization of acetylcholinesterase from Drosophila.

    Science.gov (United States)

    Gnagey, A L; Forte, M; Rosenberry, T L

    1987-09-25

    The purification and characterization of acetylcholinesterase from heads of the fruit fly Drosophila are described. Sequential extraction procedures indicated that approximately 40% of the activity was soluble and 60% membrane-bound and that virtually none (less than 4%) corresponded to collagen-tailed forms. The membrane-bound enzyme was extracted with Triton X-100 and purified over 4000-fold by affinity chromatography on acridinium resin. Hydrodynamic analysis by both sucrose gradient centrifugation and chromatography on Sepharose CL-4B revealed an Mr of 165,000 similar to that observed for dimeric (G2) forms of the enzyme in mammalian tissues. In contrast, the purified enzyme gave predominant bands of about 100 kDa prior to disulfied reduction and 55 kDa after reduction on polyacrylamide gel electrophoresis in sodium dodecyl sulfate, values that are significantly lower than those reported for purified G2 enzymes from other species. However, the presence of a faint band at 70 kDa which could be labeled by [3H]diisopropyl fluorophosphate prior to denaturation suggested that the 55-kDa band as well as a 16-kDa species arose from proteolysis. This was confirmed by reductive radiomethylation and amine analysis of the 70-, 55-, and 16-kDa bands. All three contained ethanolamine and glucosamine residues that are characteristic of a C-terminal glycolipid anchor in other G2 acetylcholinesterases. The catalytic properties of the enzyme were examined by titration with a fluorogenic reagent which revealed a turnover number for acetylthiocholine that was 6-fold lower than eel and 3-fold lower than human erythrocyte acetylcholinesterase. Furthermore, the Drosophila enzyme hydrolyzed butyrylthiocholine much more efficiently than these eel or human enzymes, an indication that the fly head enzyme has a substrate specificity intermediate between mammalian acetylcholinesterases and butyrylcholinesterases.

  1. The Evolution of Olfactory Gene Families in Drosophila and the Genomic Basis of chemical-Ecological Adaptation in Drosophila suzukii

    Science.gov (United States)

    Ramasamy, Sukanya; Ometto, Lino; Crava, Cristina M.; Revadi, Santosh; Kaur, Rupinder; Horner, David S.; Pisani, Davide; Dekker, Teun; Anfora, Gianfranco; Rota-Stabelli, Omar

    2016-01-01

    How the evolution of olfactory genes correlates with adaption to new ecological niches is still a debated topic. We explored this issue in Drosophila suzukii, an emerging model that reproduces on fresh fruit rather than in fermenting substrates like most other Drosophila. We first annotated the repertoire of odorant receptors (ORs), odorant binding proteins (OBPs), and antennal ionotropic receptors (aIRs) in the genomes of two strains of D. suzukii and of its close relative Drosophila biarmipes. We then analyzed these genes on the phylogeny of 14 Drosophila species: whereas ORs and OBPs are characterized by higher turnover rates in some lineages including D. suzukii, aIRs are conserved throughout the genus. Drosophila suzukii is further characterized by a non-random distribution of OR turnover on the gene phylogeny, consistent with a change in selective pressures. In D. suzukii, we found duplications and signs of positive selection in ORs with affinity for short-chain esters, and loss of function of ORs with affinity for volatiles produced during fermentation. These receptors—Or85a and Or22a—are characterized by divergent alleles in the European and American genomes, and we hypothesize that they may have been replaced by some of the duplicated ORs in corresponding neurons, a hypothesis reciprocally confirmed by electrophysiological recordings. Our study quantifies the evolution of olfactory genes in Drosophila and reveals an array of genomic events that can be associated with the ecological adaptations of D. suzukii. PMID:27435796

  2. Chloride channels in the plasma membrane of a foetal Drosophila cell line, S2

    DEFF Research Database (Denmark)

    Asmild, Margit; Willumsen, Niels J.

    2000-01-01

    S2 cells, Cl- Channels, Expression system, Drosophila, Inward rectifier, Outward rectifier, Patch clamp......S2 cells, Cl- Channels, Expression system, Drosophila, Inward rectifier, Outward rectifier, Patch clamp...

  3. Drosophila ARSs contain the yeast ARS consensus sequence and a replication enhancer.

    OpenAIRE

    Mills, J S; Kingsman, A J; Kingsman, S M

    1986-01-01

    A number of restriction fragments that function as autonomously replicating sequences (ARSs) in yeast have been isolated from Drosophila melanogaster DNA. The behaviour in yeast of plasmids containing Drosophila ARS elements was studied and compared to that exhibited by the archetypal yeast ARS-1 plasmid. ARS functions were localised by subcloning and BAL-31 deletion analysis. These studies demonstrated the structural and functional complexity of Drosophila ARSs. Each Drosophila ARS element h...

  4. FlyBase: a Drosophila database. The FlyBase consortium.

    OpenAIRE

    Gelbart, W. M.; Crosby, M.; Matthews, B; Rindone, W P; Chillemi, J; Russo Twombly, S; Emmert, D.; Ashburner, M; Drysdale, R A; Whitfield, E; Millburn, G H; Grey, A; Kaufman, T; Matthews, K.; Gilbert, D

    1997-01-01

    FlyBase is a database of genetic and molecular data concerning Drosophila. FlyBase is maintained as a relational database (in Sybase) and is made available as html documents and flat files. The scope of FlyBase includes: genes, alleles (and phenotypes), aberrations, transposons, pointers to sequence data, clones, stock lists, Drosophila workers and bibliographic references. The Encyclopedia of Drosophila is a joint effort between FlyBase and the Berkeley Drosophila Genome Project which integr...

  5. An expressed sequence tag (EST library for Drosophila serrata, a model system for sexual selection and climatic adaptation studies

    Directory of Open Access Journals (Sweden)

    McGraw Elizabeth A

    2009-01-01

    Full Text Available Abstract Background The native Australian fly Drosophila serrata belongs to the highly speciose montium subgroup of the melanogaster species group. It has recently emerged as an excellent model system with which to address a number of important questions, including the evolution of traits under sexual selection and traits involved in climatic adaptation along latitudinal gradients. Understanding the molecular genetic basis of such traits has been limited by a lack of genomic resources for this species. Here, we present the first expressed sequence tag (EST collection for D. serrata that will enable the identification of genes underlying sexually-selected phenotypes and physiological responses to environmental change and may help resolve controversial phylogenetic relationships within the montium subgroup. Results A normalized cDNA library was constructed from whole fly bodies at several developmental stages, including larvae and adults. Assembly of 11,616 clones sequenced from the 3' end allowed us to identify 6,607 unique contigs, of which at least 90% encoded peptides. Partial transcripts were discovered from a variety of genes of evolutionary interest by BLASTing contigs against the 12 Drosophila genomes currently sequenced. By incorporating into the cDNA library multiple individuals from populations spanning a large portion of the geographical range of D. serrata, we were able to identify 11,057 putative single nucleotide polymorphisms (SNPs, with 278 different contigs having at least one "double hit" SNP that is highly likely to be a real polymorphism. At least 394 EST-associated microsatellite markers, representing 355 different contigs, were also found, providing an additional set of genetic markers. The assembled EST library is available online at http://www.chenowethlab.org/serrata/index.cgi. Conclusion We have provided the first gene collection and largest set of polymorphic genetic markers, to date, for the fly D. serrata. The EST

  6. The dopaminergic system in the aging brain of Drosophila

    Directory of Open Access Journals (Sweden)

    Katherine E White

    2010-12-01

    Full Text Available Drosophila models of Parkinson’s disease are characterised by two principal phenotypes: the specific loss of dopaminergic neurons in the aging brain and defects in motor behavior. However, an age-related analysis of these baseline parameters in wildtype Drosophila is lacking. Here we analysed the dopaminergic system and motor behavior in aging Drosophila. Dopaminergic neurons in the adult brain can be grouped into bilateral symmetric clusters, each comprising a stereotypical number of cells. Analysis of TH>mCD8::GFP and cell type-specific MARCM clones revealed that dopaminergic neurons show cluster-specific, stereotypical projection patterns with terminal arborization in target regions that represent distinct functional areas of the adult brain. Target areas include the mushroom bodies, involved in memory formation and motivation, and the central complex, involved in the control of motor behavior, indicating that similar to the mammalian brain, dopaminergic neurons in the fly brain are involved in the regulation of specific behaviors. Behavioral analysis revealed that Drosophila show an age-related decline in startle-induced locomotion and negative geotaxis. Motion tracking however, revealed that walking activity and exploration behavior, but not centrophobism increase at late stages of life. Analysis of TH>Dcr2, mCD8::GFP revealed a specific effect of Dcr2 expression on walking activity but not on exploratory or centrophobic behavior, indicating that the siRNA pathway may modulate distinct dopaminergic behaviors in Drosophila. Moreover, dopaminergic neurons were maintained between early- and late life, as quantified by TH>mCD8::GFP and anti-TH labelling, indicating that adult onset, age-related degeneration of dopaminergic neurons does not occur in the aging brain of Drosophila. Taken together, our data establish baseline parameters in Drosophila for the study of Parkinson’s disease as well as other disorders affecting dopaminergic neurons

  7. Variable phenotype of Marfan syndrome in two large Australian pedigrees, one of Australian aboriginal origin

    Energy Technology Data Exchange (ETDEWEB)

    Wong, K.K.; Summers, K.M.; West, M.J. [Univ. of Queensland (Australia)] [and others

    1994-09-01

    Marfan syndrome may affect the cardiovascular, ocular and skeletal systems. The gene for this autosomal dominant disease maps to chromosome 15 and codes for the extracellular matrix protein fibrillin. Phenotypic expression is very variable both within and between families, possibly due to the influence of other, unlinked, genetic factors interacting with the fibrillin gene. We report two Australian families which demonstrate the extent of inter- and intra-family phenotypic variability. Eye, cardiac and skeletal assessments were made independently. In the first family, 8 of 12 siblings and 11 of 19 of their children had ectopia lentis with or without other ocular findings. There were few cardiac signs. One child had mitral valve prolapse. He and three other children had mild dilatation of the aorta. Skeletal abnormalities were also found (3 adults and 7 children). Chest wall asymmetry was the most common skeletal finding. This family has less cardiac and skeletal involvement than is usual in Marfan syndrome, although the disease maps to chromosome 15 in the region of the fibrillin gene (LOD=4.8 at {theta}=0 with respect to CYP19). The second family is partly of Australian aboriginal origin. The disease has been traced through 5 generations. To date we have examined 37 of 84 living members. Twenty-three in 3 generations are affected. Five adults and 4 children have moderate to severe aortic dilatation and there has been at least one death due to aortic dissection. However, two adolescents with subluxed lenses and marked skeletal abnormalities have normal aortic diameters, two children have aortic dilatation without other signs and two children have only subluxed lenses. This family shows the range of phenotypic variation which can arise from mutation in the fibrillin gene, which may be influenced by the admixture of Australian aboriginal genes. These two families provide an invaluable resource for studying genetic interactions in this disease.

  8. The Australian Integrated Marine Observing System

    Science.gov (United States)

    Proctor, R.; Meyers, G.; Roughan, M.; Operators, I.

    2008-12-01

    The Integrated Marine Observing System (IMOS) is a 92M project established with 50M from the National Collaborative Research Infrastructure Strategy (NCRIS) and co-investments from 10 operators including Universities and government agencies (see below). It is a nationally distributed set of equipment established and maintained at sea, oceanographic data and information services that collectively will contribute to meeting the needs of marine research in both open oceans and over the continental shelf around Australia. In particular, if sustained in the long term, it will permit identification and management of climate change in the marine environment, an area of research that is as yet almost a blank page, studies relevant to conservation of marine biodiversity and research on the role of the oceans in the climate system. While as an NCRIS project IMOS is intended to support research, the data streams are also useful for many societal, environmental and economic applications, such as management of offshore industries, safety at sea, management of marine ecosystems and fisheries and tourism. The infrastructure also contributes to Australia's commitments to international programs of ocean observing and international conventions, such as the 1982 Law of the Sea Convention that established the Australian Exclusive Economic Zone, the United Nations Framework Convention on Climate Change, the Global Ocean Observing System and the intergovernmental coordinating activity Global Earth Observation System of Systems. IMOS is made up of nine national facilities that collect data, using different components of infrastructure and instruments, and two facilities that manage and provide access to data and enhanced data products, one for in situ data and a second for remotely sensed satellite data. The observing facilities include three for the open (bluewater) ocean (Argo Australia, Enhanced Ships of Opportunity and Southern Ocean Time Series), three facilities for coastal

  9. Modelling seasonality in Australian building approvals

    Directory of Open Access Journals (Sweden)

    Harry M Karamujic

    2012-02-01

    Full Text Available The paper examines the impact of seasonal influences on Australian housing approvals, represented by the State of Victoria[1] building approvals for new houses (BANHs. The prime objective of BANHs is to provide timely estimates of future residential building work. Due to the relevance of the residential property sector to the property sector as whole, BANHs are viewed by economic analysts and commentators as a leading indicator of property sector investment and as such the general level of economic activity and employment. The generic objective of the study is to enhance the practice of modelling housing variables. In particular, the study seeks to cast some additional light on modelling the seasonal behaviour of BANHs by: (i establishing the presence, or otherwise, of seasonality in Victorian BANHs; (ii if present, ascertaining is it deterministic or stochastic; (iii determining out of sample forecasting capabilities of the considered modelling specifications; and (iv speculating on possible interpretation of the results. To do so the study utilises a structural time series model of Harwey (1989. The modelling results confirm that the modelling specification allowing for stochastic trend and deterministic seasonality performs best in terms of diagnostic tests and goodness of fit measures. This is corroborated with the analysis of out of sample forecasting capabilities of the considered modelling specifications, which showed that the models with deterministic seasonal specification exhibit superior forecasting capabilities. The paper also demonstrates that if time series are characterized by either stochastic trend or seasonality, the conventional modelling approach[2] is bound to be mis-specified i.e. would not be able to identify statistically significant seasonality in time series.According to the selected modeling specification, factors corresponding to June, April, December and November are found to be significant at five per cent level

  10. Injection Efficiency Monitor for the Australian Synchrotron

    Directory of Open Access Journals (Sweden)

    Rassool R. P.

    2012-10-01

    Full Text Available The Australian Synchrotron AS is moving towards a continuous injection mode called top-up. During top-up the linac and booster synchrotron injection system will be in continuous operation rather than usedevery eight hours the way they are used at present. In order to monitor the performance of the injection system areal-time injection efficiency monitoring system has been developed. The system consists of several Fast CurrentTransformers [1] and matching digitisers [2] and is designed to count every beam pulse and measure the transmission efficiency through the whole accelerator complex. After calibrating the system using a properly matchedFaraday Cup at the electron gun, a transmission efficiency is then calculated at each stage of transferring the beamfrom 90 keV out of the gun to 3 GeV in the storage ring. The system is used to optimise the injection process inorder to maximise the injection efficiency and as an early warning system when equipment starts to fail and theinjection efficiency decreases.

  11. Effective teaching strategies in Australian multicultural classroom

    Institute of Scientific and Technical Information of China (English)

    高芳卉

    2011-01-01

    @@ 1 Introduction@@ Australia's population is increasingly culturally diverse.The diverse composition of the Australian population is reflected in the ACT.The 1991 census showed 65,739 people counted in the ACT were bern overseas,representing 23.5% of the population.Almost 10% of the respondents to the census came from non-English - speaking countries.(1) The results from the 2001 census showed that of the 4,645,000 people in Victoria,almost one quarter (23.4%) were born overseas,represented 208 countries and spoke 151 languages.English was spoken at home by 75.3% of Victorians.(2) These numbers are reflected in our schools because students come from many cultural,educational and language backgrounds.The increasingly muhieultural populations in our schools present many challenges for schools and teachers,with regards to inclusive teaching strategies,language differences,muhieuhural curricular practices,racism issues and numerous other factors.

  12. Cancer incidence in Australian Vietnam veterans

    Energy Technology Data Exchange (ETDEWEB)

    Wilson, E.; Horsley, K. [Australian Government Department of Veterans' Affairs (Australia); Hoek, R. van der [Australian Institute of Health and Welfare (Australia)

    2004-09-15

    Australian Defence Force (ADF) personnel participated in the Vietnam Conflict from 1962 to 1973, involving nearly 60,000 personnel, of whom over 500 died during service and 3131 were severely physically wounded. Service in the Vietnam conflict presented distinct health challenges. Besides the hazards of combat conditions for extended periods, herbicides and other toxic chemicals were used extensively. The United States military sprayed more than 76,000,000L of herbicide over Vietnam in their Air Force Ranch Hand and Operation Trail Dust programs. The most heavily used herbicide was Agent Orange, contaminated with 2,3,7,8-tetrachlorodibenzo-pdioxin. Since the Vietnam conflict, ex-Service organisations (ESOs) have maintained that Vietnam service adversely affected the health of veterans. Initial studies showed no excess risk attributable to their service. However, more recent studies have shown that Vietnam veterans have excess incidence and mortality rates from several conditions such as cancers and heart disease. This paper describes the first cancer incidence study for all ADF Vietnam veterans.

  13. Bt resistance in Australian insect pest species.

    Science.gov (United States)

    Downes, Sharon; Walsh, Tom; Tay, Wee Tek

    2016-06-01

    Bt cotton was initially deployed in Australia in the mid-1990s to control the polyphagous pest Helicoverpa armigera (Hübner) which was intractably resistant to synthetic chemistries. A conservative strategy was enforced and resistance to first generation single toxin technology was managed. A decade later, shortly after the release of dual toxin cotton, high baseline frequencies of alleles conferring resistance to one of its components prompted a reassessment of the thinking behind the potential risks to this technology. Several reviews detail the characteristics of this resistance and the nuances of deploying first and second generation Bt cotton in Australia. Here we explore recent advances and future possibilities to estimate Bt resistance in Australian pest species and define what we see as the critical data for enabling effective pre-emptive strategies. We also foreshadow the imminent deployment of three toxin (Cry1Ac, Cry2Ab, Vip3A) Bollgard 3 cotton, and examine aspects of resistance to its novel component, Vip3A, that we believe may impact on its stewardship. PMID:27436735

  14. Australians Abroad: Narrative Paths and Divagations

    Directory of Open Access Journals (Sweden)

    Juliana de Nooy

    2013-01-01

    Full Text Available Although commonly characterized as an immigrant nation, Australia has been shaped just as importantly by the overseas journeys of its people, and the liminal experiences thus provided have not only been self-defining and defining of the other, but at times nation-defining. This special issue proposes a multidisciplinary analysis of Australian travellers and expatriates past and present: the reasons for and destinations of their travel, its impact on their identity, the roles they play, their writings and reflections, their linguistic and intercultural competence. Clusters of travellers to particular destinations give rise to narrative patterns which solidify into templates, the narrative equivalent of the beaten track. The essays that follow highlight both discursive grooves and off-piste accounts that challenge the patterns. In both cases, the emphasis in the essays is on the travellers’ active engagement in the experience and on their negotiation of existing discourses. For even those who follow the trail invest it with personal meanings.

  15. Nutrition and general practice: an Australian perspective.

    Science.gov (United States)

    Helman, A

    1997-06-01

    Australia has a government-subsidized, private medical system in which general practitioners (GPs) form the core component of primary care. There are approximately 20,000 active GPs and 80% of the population consults a GP each year. A new vocational register of GPs has been set up that requires training in general practice, followed by formal continuing education. I briefly review sources of information about Australian GPs' practices and knowledge of and attitudes toward nutrition. About 15-17% of GPs say they have a special interest in nutrition (20% of female GPs and 13% of male GPs). The main conditions for which advice is given are heart disease, hyperlipidemia, obesity, and diabetes. The extent of nutrition counseling by GPs is considerably less than might be expected from the strength of their statements about the importance of nutrition and long-term health. Obstacles to nutrition counseling are lack of time, lack of confidence, and inadequate nutrition knowledge, the last documented by objective testing. GPs express interest in learning more about nutrition (which may be partly driven by consumer pressure) but there is still little coherent teaching on the subject, specifically tailored for GPs. When asked their preferences for nutrition education, GPs tend to prefer educational material (such as diet charts) to give to patients. PMID:9174498

  16. Malignant otitis externa: An Australian case series.

    LENUS (Irish Health Repository)

    2012-02-01

    OBJECTIVES: To establish a clinicopathological profile of malignant otitis externa (MOE) in an Australian tertiary referral institution. STUDY DESIGN: Retrospective cohort outcomes study. METHODS: 24 patients were identified with MOE between January 1998 and July 2007. Patients were classified into Radiological Grades I-IV. Laboratory investigations Including C-reactive protein (CRP), white cell count (WCC), glycosylated haemoglobin (HBA1c) and average glucose level over admission were recorded. RESULTS: Radiological Grade was significantly associated with duration of therapy (rank correlation 0.57, p = 0.004). CRP was a useful indicator confirming disease resolution. Diabetics with MOE had elevated average blood sugar levels during their Hospital admission (p < 0.001) and had poor overall glycaemic control represented by Elevated HBA1c scores (p < 0.001). CONCLUSIONS: Malignant otitis externa is a rare disease, which is best managed in a multidisciplinary team setting. This practical grading system can be used to predict the duration of therapy at time of diagnosis, which enables the efficient utilisation of Hospital resources. Poorly controlled diabetics are more susceptible to developing. MOE than diabetics with satisfactory glycaemic control and may represent a subgroup of more brittle diabetics. CRP combined with appropriate clinical and radiological investigations is useful in assessing disease resolution.

  17. Adolescent energy drink consumption: An Australian perspective.

    Science.gov (United States)

    Costa, Beth M; Hayley, Alexa; Miller, Peter

    2016-10-01

    Caffeinated Energy Drinks (EDs) are not recommended for consumption by children, yet there is a lack of age-specific recommendations and restrictions on the marketing and sale of EDs. EDs are increasingly popular among adolescents despite growing evidence of their negative health effects. In the current study we examined ED consumption patterns among 399 Australian adolescents aged 12-18 years. Participants completed a self-report survey of consumption patterns, physiological symptoms, and awareness of current ED consumption guidelines. Results indicated that ED consumption was common among the sample; 56% reported lifetime ED consumption, with initial consumption at mean age 10 (SD = 2.97). Twenty-eight percent of the sample consumed EDs at least monthly, 36% had exceeded the recommended two standard EDs/day, and 56% of consumers had experienced negative physiological health effects following ED consumption. The maximum number of EDs/day considered appropriate for children, adolescents, and adults varied, indicating a lack of awareness of current consumption recommendations. These findings add to the growing body of international evidence of adolescent ED consumption, and the detrimental impact of EDs to adolescent health. Enforced regulation and restriction of EDs for children's and adolescents' consumption is urgently needed in addition to greater visibility of ED consumption recommendations. PMID:27389033

  18. The colorful language of Australian flowers.

    Science.gov (United States)

    Burd, Martin; Stayton, C Tristan; Shrestha, Mani; Dyer, Adrian G

    2014-01-01

    The enormous increase in phylogenetic information in recent years has allowed many old questions to be reexamined from a macroevolutionary perspective. We have recently considered evolutionary convergence in floral colors within pollination syndromes, using bird-pollinated species in Australia. We combined quantitative measurements of floral reflectance spectra, models of avian color vision, and a phylogenetic tree of 234 Australian species to show that bird-pollinated flowers as a group do not have colors that are significantly different from the colors of insect-pollinated flowers. However, about half the bird-pollinated flowers have convergently evolved a narrow range of colors with dominant long-wavelength reflection far more often than would be expected by chance. These convergent colors would be seen as distinctly different from other floral colors in our sample when viewed by honeyeaters (family Meliphagidae), birds with a phylogenetically ancestral type of color vision and the dominant avian pollinators in Australia. Our analysis shows how qualitative ideas in natural history, like the concept of pollination syndromes, can be given more precise definition and rigorous statistical testing that takes into account phylogenetic information. PMID:25346795

  19. Drosophila wing modularity revisited through a quantitative genetic approach.

    Science.gov (United States)

    Muñoz-Muñoz, Francesc; Carreira, Valeria Paula; Martínez-Abadías, Neus; Ortiz, Victoria; González-José, Rolando; Soto, Ignacio M

    2016-07-01

    To predict the response of complex morphological structures to selection it is necessary to know how the covariation among its different parts is organized. Two key features of covariation are modularity and integration. The Drosophila wing is currently considered a fully integrated structure. Here, we study the patterns of integration of the Drosophila wing and test the hypothesis of the wing being divided into two modules along the proximo-distal axis, as suggested by developmental, biomechanical, and evolutionary evidence. To achieve these goals we perform a multilevel analysis of covariation combining the techniques of geometric morphometrics and quantitative genetics. Our results indicate that the Drosophila wing is indeed organized into two main modules, the wing base and the wing blade. The patterns of integration and modularity were highly concordant at the phenotypic, genetic, environmental, and developmental levels. Besides, we found that modularity at the developmental level was considerably higher than modularity at other levels, suggesting that in the Drosophila wing direct developmental interactions are major contributors to total phenotypic shape variation. We propose that the precise time at which covariance-generating developmental processes occur and/or the magnitude of variation that they produce favor proximo-distal, rather than anterior-posterior, modularity in the Drosophila wing. PMID:27272402

  20. big bang gene modulates gut immune tolerance in Drosophila.

    Science.gov (United States)

    Bonnay, François; Cohen-Berros, Eva; Hoffmann, Martine; Kim, Sabrina Y; Boulianne, Gabrielle L; Hoffmann, Jules A; Matt, Nicolas; Reichhart, Jean-Marc

    2013-02-19

    Chronic inflammation of the intestine is detrimental to mammals. Similarly, constant activation of the immune response in the gut by the endogenous flora is suspected to be harmful to Drosophila. Therefore, the innate immune response in the gut of Drosophila melanogaster is tightly balanced to simultaneously prevent infections by pathogenic microorganisms and tolerate the endogenous flora. Here we describe the role of the big bang (bbg) gene, encoding multiple membrane-associated PDZ (PSD-95, Discs-large, ZO-1) domain-containing protein isoforms, in the modulation of the gut immune response. We show that in the adult Drosophila midgut, BBG is present at the level of the septate junctions, on the apical side of the enterocytes. In the absence of BBG, these junctions become loose, enabling the intestinal flora to trigger a constitutive activation of the anterior midgut immune response. This chronic epithelial inflammation leads to a reduced lifespan of bbg mutant flies. Clearing the commensal flora by antibiotics prevents the abnormal activation of the gut immune response and restores a normal lifespan. We now provide genetic evidence that Drosophila septate junctions are part of the gut immune barrier, a function that is evolutionarily conserved in mammals. Collectively, our data suggest that septate junctions are required to maintain the subtle balance between immune tolerance and immune response in the Drosophila gut, which represents a powerful model to study inflammatory bowel diseases. PMID:23378635

  1. big bang gene modulates gut immune tolerance in Drosophila

    Science.gov (United States)

    Bonnay, François; Cohen-Berros, Eva; Hoffmann, Martine; Kim, Sabrina Y.; Boulianne, Gabrielle L.; Hoffmann, Jules A.; Matt, Nicolas; Reichhart, Jean-Marc

    2013-01-01

    Chronic inflammation of the intestine is detrimental to mammals. Similarly, constant activation of the immune response in the gut by the endogenous flora is suspected to be harmful to Drosophila. Therefore, the innate immune response in the gut of Drosophila melanogaster is tightly balanced to simultaneously prevent infections by pathogenic microorganisms and tolerate the endogenous flora. Here we describe the role of the big bang (bbg) gene, encoding multiple membrane-associated PDZ (PSD-95, Discs-large, ZO-1) domain-containing protein isoforms, in the modulation of the gut immune response. We show that in the adult Drosophila midgut, BBG is present at the level of the septate junctions, on the apical side of the enterocytes. In the absence of BBG, these junctions become loose, enabling the intestinal flora to trigger a constitutive activation of the anterior midgut immune response. This chronic epithelial inflammation leads to a reduced lifespan of bbg mutant flies. Clearing the commensal flora by antibiotics prevents the abnormal activation of the gut immune response and restores a normal lifespan. We now provide genetic evidence that Drosophila septate junctions are part of the gut immune barrier, a function that is evolutionarily conserved in mammals. Collectively, our data suggest that septate junctions are required to maintain the subtle balance between immune tolerance and immune response in the Drosophila gut, which represents a powerful model to study inflammatory bowel diseases. PMID:23378635

  2. Intestinal stem cells in the adult Drosophila midgut

    Energy Technology Data Exchange (ETDEWEB)

    Jiang, Huaqi, E-mail: Huaqi.Jiang@UTSouthwestern.edu [Department of Developmental Biology, UT Southwestern Medical Center, 6000 Harry Hines Blvd., Dallas, TX, 75235 (United States); Edgar, Bruce A., E-mail: b.edgar@dkfz.de [ZMBH-DKFZ Alliance, Im Neuenheimer Feld 282, D-69120 Heidelberg (Germany); Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Seattle, WA 98109 (United States)

    2011-11-15

    Drosophila has long been an excellent model organism for studying stem cell biology. Notably, studies of Drosophila's germline stem cells have been instrumental in developing the stem cell niche concept. The recent discovery of somatic stem cells in adult Drosophila, particularly the intestinal stem cells (ISCs) of the midgut, has established Drosophila as an exciting model to study stem cell-mediated adult tissue homeostasis and regeneration. Here, we review the major signaling pathways that regulate the self-renewal, proliferation and differentiation of Drosophila ISCs, discussing how this regulation maintains midgut homeostasis and mediates regeneration of the intestinal epithelium after injury. -- Highlights: Black-Right-Pointing-Pointer The homeostasis and regeneration of adult fly midguts are mediated by ISCs. Black-Right-Pointing-Pointer Damaged enterocytes induce the proliferation of intestinal stem cells (ISC). Black-Right-Pointing-Pointer EGFR and Jak/Stat signalings mediate compensatory ISC proliferation. Black-Right-Pointing-Pointer Notch signaling regulates ISC self-renewal and differentiation.

  3. Transmembrane channel-like (tmc) gene regulates Drosophila larval locomotion.

    Science.gov (United States)

    Guo, Yanmeng; Wang, Yuping; Zhang, Wei; Meltzer, Shan; Zanini, Damiano; Yu, Yue; Li, Jiefu; Cheng, Tong; Guo, Zhenhao; Wang, Qingxiu; Jacobs, Julie S; Sharma, Yashoda; Eberl, Daniel F; Göpfert, Martin C; Jan, Lily Yeh; Jan, Yuh Nung; Wang, Zuoren

    2016-06-28

    Drosophila larval locomotion, which entails rhythmic body contractions, is controlled by sensory feedback from proprioceptors. The molecular mechanisms mediating this feedback are little understood. By using genetic knock-in and immunostaining, we found that the Drosophila melanogaster transmembrane channel-like (tmc) gene is expressed in the larval class I and class II dendritic arborization (da) neurons and bipolar dendrite (bd) neurons, both of which are known to provide sensory feedback for larval locomotion. Larvae with knockdown or loss of tmc function displayed reduced crawling speeds, increased head cast frequencies, and enhanced backward locomotion. Expressing Drosophila TMC or mammalian TMC1 and/or TMC2 in the tmc-positive neurons rescued these mutant phenotypes. Bending of the larval body activated the tmc-positive neurons, and in tmc mutants this bending response was impaired. This implicates TMC's roles in Drosophila proprioception and the sensory control of larval locomotion. It also provides evidence for a functional conservation between Drosophila and mammalian TMCs. PMID:27298354

  4. Research progress on Drosophila visual cognition in China

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Visual cognition,as one of the fundamental aspects of cognitive neuroscience,is generally associated with high-order brain functions in animals and human.Drosophila,as a model organism,shares certain features of visual cognition in common with mammals at the genetic,molecular,cellular,and even higher behavioral levels.From learning and memory to decision making,Drosophila covers a broad spectrum of higher cognitive behaviors beyond what we had expected.Armed with powerful tools of genetic manipulation in Drosophila,an increasing number of studies have been conducted in order to elucidate the neural circuit mechanisms underlying these cognitive behaviors from a genes-brain-behavior perspective.The goal of this review is to integrate the most important studies on visual cognition in Drosophila carried out in mainland China during the last decade into a body of knowledge encompassing both the basic neural operations and circuitry of higher brain function in Drosophila.Here,we consider a series of the higher cognitive behaviors beyond learning and memory,such as visual pattern recognition,feature and context generalization,different feature memory traces,salience-based decision,attention-like behavior,and cross-modal leaning and memory.We discuss the possible general gain-gating mechanism implementing by dopamine-mushroom body circuit in fly’s visual cognition.We hope that our brief review on this aspect will inspire further study on visual cognition in flies,or even beyond.

  5. Biological radiation effects of Radon in Drosophila

    International Nuclear Information System (INIS)

    In order to contribute to the knowledge on the effects of radon and its decay products, the aim of this investigation is to study the biological effects of radon using Drosophila melanogaster throught the somatic mutation and recombination test (SMART) and the analysis of some adaptative factors exposing larvaes to controlled radon atmosphers, considering that this insect could be used as biological monitor. Using the somatic mutation test a mutagenic effect was observed proportional to radon concentration, into an interval of 1 ± 0.3 to 111 ± 7.4 KBq/m3 equivalent to doses under 0.0106 Gy. The correlation analysis gives a linear (r=0.80) relationship with a positive slope of 0.2217. The same happens when gamma rays are used in the interval of 1 to 20 Gy, given a linear dose-dependent effect (r=0.878) is obtained; nevetheless the slop is smaller (m=0.003) than for radon. Analysing the results of adaptative factors of the nine exposed generations, it was found that probably radon exposition induced dominant lethals during gametogenesis or/and a selection of the more component gamets of the treated individuals in larval state. It was reflected in the significant decrease on fecundity of the generation exposed. Nevertheless the laying eggs had an increase in egg-to-adult viability and the develop velocity was higher than in control for 3 KBq/m3, this suggest that radon concentrations used were able to induce repair mechanisms. These data agree with the Hormesis hypothesis that says: low doses have positive effects on health. It was not possible to obtain a dose-effect relationship except with the develop velocity where it was found a dose-effect inverse proportion. In conclusion, Drosophila melanogaster could be a good system to obtain in vivo damaged induction concentration dependent of radon and its decay products, as well as to study the effects in an exposed population by the analysis of adaptative factors. (Author)

  6. Operation of the Australian Store.Synchrotron for macromolecular crystallography

    Energy Technology Data Exchange (ETDEWEB)

    Meyer, Grischa R. [Monash University, Clayton, Victoria 3800 (Australia); Aragão, David; Mudie, Nathan J.; Caradoc-Davies, Tom T. [Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria 3168 (Australia); McGowan, Sheena; Bertling, Philip J.; Groenewegen, David; Quenette, Stevan M. [Monash University, Clayton, Victoria 3800 (Australia); Bond, Charles S. [The University of Western Australia, 35 Stirling Highway, Crawley 6009, Western Australia (Australia); Buckle, Ashley M. [Monash University, Clayton, Victoria 3800 (Australia); Androulakis, Steve, E-mail: steve.androulakis@monash.edu [Monash Bioinformatics Platform, Monash University, Clayton, Victoria 3800 (Australia)

    2014-10-01

    The Store.Synchrotron service, a fully functional, cloud computing-based solution to raw X-ray data archiving and dissemination at the Australian Synchrotron, is described. The Store.Synchrotron service, a fully functional, cloud computing-based solution to raw X-ray data archiving and dissemination at the Australian Synchrotron, is described. The service automatically receives and archives raw diffraction data, related metadata and preliminary results of automated data-processing workflows. Data are able to be shared with collaborators and opened to the public. In the nine months since its deployment in August 2013, the service has handled over 22.4 TB of raw data (∼1.7 million diffraction images). Several real examples from the Australian crystallographic community are described that illustrate the advantages of the approach, which include real-time online data access and fully redundant, secure storage. Discoveries in biological sciences increasingly require multidisciplinary approaches. With this in mind, Store.Synchrotron has been developed as a component within a greater service that can combine data from other instruments at the Australian Synchrotron, as well as instruments at the Australian neutron source ANSTO. It is therefore envisaged that this will serve as a model implementation of raw data archiving and dissemination within the structural biology research community.

  7. An expanded prescribing role for pharmacists – an Australian perspective

    Directory of Open Access Journals (Sweden)

    Kreshnik Hoti

    2011-04-01

    Full Text Available Expanded pharmacist prescribing is a new professionalpractice area for pharmacists. Currently, Australianpharmacists’ prescribing role is limited to over-the-countermedications. This review aims to identify Australian studiesinvolving the area of expanded pharmacist prescribing.Australian studies exploring the issues of pharmacistprescribing were identified and considered in the context ofits implementation internationally. Australian studies havemainly focused on the attitudes of community and hospitalpharmacists towards such an expansion. Studies evaluatingthe views of Australian consumers and pharmacy clients werealso considered. The available Australian literature indicatedsupport from pharmacists and pharmacy clients for anexpanded pharmacist prescribing role, with preference fordoctors retaining a primary role in diagnosis. Australianpharmacists and pharmacy client’s views were also inagreement in terms of other key issues surrounding expandedpharmacist prescribing. These included the nature of anexpanded prescribing model, the need for additional trainingfor pharmacists and the potential for pharmacy clients gainingimproved medication access, which could be achieved withinan expanded role that pharmacists could provide. Currentevidence from studies conducted in Australia providesvaluable insight to relevant policymakers on the issue ofpharmacist prescribing in order to move the agenda ofpharmacist prescribing forwards.

  8. Genomics of clinal variation in Drosophila: disentangling the interactions of selection and demography.

    Science.gov (United States)

    Flatt, Thomas

    2016-03-01

    Clines in phenotypes and genotype frequencies across environmental gradients are commonly taken as evidence for spatially varying selection. Classical examples include the latitudinal clines in various species of Drosophila, which often occur in parallel fashion on multiple continents. Today, genomewide analysis of such clinal systems provides a fantastic opportunity for unravelling the genetics of adaptation, yet major challenges remain. A well-known but often neglected problem is that demographic processes can also generate clinality, independent of or coincident with selection. A closely related issue is how to identify true genic targets of clinal selection. In this issue of Molecular Ecology, three studies illustrate these challenges and how they might be met. Bergland et al. report evidence suggesting that the well-known parallel latitudinal clines in North American and Australian D. melanogaster are confounded by admixture from Africa and Europe, highlighting the importance of distinguishing demographic from adaptive clines. In a companion study, Machado et al. provide the first genomic comparison of latitudinal differentiation in D. melanogaster and its sister species D. simulans. While D. simulans is less clinal than D. melanogaster, a significant fraction of clinal genes is shared between both species, suggesting the existence of convergent adaptation to clinaly varying selection pressures. Finally, by drawing on several independent sources of evidence, Božičević et al. identify a functional network of eight clinal genes that are likely involved in cold adaptation. Together, these studies remind us that clinality does not necessarily imply selection and that separating adaptive signal from demographic noise requires great effort and care. PMID:26919307

  9. Mechanics, Problems and Contributions of Tertiary Strategic Alliance: The Case of 22 Australian Universities.

    Science.gov (United States)

    Saffu, Kojo; Mamman, Aminu

    1999-01-01

    A study of international strategic alliances involving 22 Australian universities indicates that a majority of universities have frameworks for internationalization initiatives, with top institutional management instrumental in initiating joint ventures with overseas institutions despite limited resources. Australian universities believe they…

  10. Comparative population genomics of latitudinal variation in Drosophila simulans and Drosophila melanogaster.

    Science.gov (United States)

    Machado, Heather E; Bergland, Alan O; O'Brien, Katherine R; Behrman, Emily L; Schmidt, Paul S; Petrov, Dmitri A

    2016-02-01

    Examples of clinal variation in phenotypes and genotypes across latitudinal transects have served as important models for understanding how spatially varying selection and demographic forces shape variation within species. Here, we examine the selective and demographic contributions to latitudinal variation through the largest comparative genomic study to date of Drosophila simulans and Drosophila melanogaster, with genomic sequence data from 382 individual fruit flies, collected across a spatial transect of 19 degrees latitude and at multiple time points over 2 years. Consistent with phenotypic studies, we find less clinal variation in D. simulans than D. melanogaster, particularly for the autosomes. Moreover, we find that clinally varying loci in D. simulans are less stable over multiple years than comparable clines in D. melanogaster. D. simulans shows a significantly weaker pattern of isolation by distance than D. melanogaster and we find evidence for a stronger contribution of migration to D. simulans population genetic structure. While population bottlenecks and migration can plausibly explain the differences in stability of clinal variation between the two species, we also observe a significant enrichment of shared clinal genes, suggesting that the selective forces associated with climate are acting on the same genes and phenotypes in D. simulans and D. melanogaster. PMID:26523848

  11. Quantifying host potentials: indexing postharvest fresh fruits for spotted wing Drosophila, Drosophila suzukii.

    Directory of Open Access Journals (Sweden)

    David E Bellamy

    Full Text Available Novel methodology is presented for indexing the relative potential of hosts to function as resources. A Host Potential Index (HPI was developed as a practical framework to express relative host potential based on combining results from one or more independent studies, such as those examining host selection, utilization, and physiological development of the organism resourcing the host. Several aspects of the HPI are addressed including: 1 model derivation; 2 influence of experimental design on establishing host rankings for a study type (no choice, two-choice, and multiple-choice; and, 3 variable selection and weighting associated with combining multiple studies. To demonstrate application of the HPI, results from the interactions of spotted wing drosophila (SWD, Drosophila suzukii Matsumura (Diptera: Drosophilidae, with seven "reported" hosts (blackberries, blueberries, sweet cherries, table grapes, peaches, raspberries, and strawberries in a postharvest scenario were analyzed. Four aspects of SWD-host interaction were examined: attraction to host volatiles; population-level oviposition performance; individual-level oviposition performance; and key developmental factors. Application of HPI methodology indicated that raspberries ( (meanHPIvaried  = 301.9±8.39; rank 1 of 7 have the greatest potential to serve as a postharvest host for SWD relative to the other fruit hosts, with grapes ( (meanHPIvaried  = 232.4±3.21; rank 7 of 7 having the least potential.

  12. Patterns of mutation and selection at synonymous sites in Drosophila

    DEFF Research Database (Denmark)

    Singh, Nadia D; Bauer DuMont, Vanessa L; Hubisz, Melissa J;

    2007-01-01

    That natural selection affects molecular evolution at synonymous sites in protein-coding sequences is well established and is thought to predominantly reflect selection for translational efficiency/accuracy mediated through codon bias. However, a recently developed maximum likelihood framework...... be subject to a variety of selective pressures beyond weak selection for increased frequencies of the codons currently defined as "preferred" in D. melanogaster. To further explore patterns of synonymous site evolution in Drosophila in a lineage-specific manner, we expanded the application of the maximum......, when applied to 18 coding sequences in 3 species of Drosophila, confirmed an earlier report that the Notch gene in Drosophila melanogaster was evolving under selection in favor of those codons defined as unpreferred in this species. This finding opened the possibility that synonymous sites may...

  13. On the origin of new genes in Drosophila

    DEFF Research Database (Denmark)

    Zhou, Qi; Zhang, Guojie; Zhang, Yue;

    2008-01-01

    Several mechanisms have been proposed to account for the origination of new genes. Despite extensive case studies, the general principles governing this fundamental process are still unclear at the whole-genome level. Here, we unveil genome-wide patterns for the mutational mechanisms leading to new...... genes and their subsequent lineage-specific evolution at different time nodes in the Drosophila melanogaster species subgroup. We find that (1) tandem gene duplication has generated approximately 80% of the nascent duplicates that are limited to single species (D. melanogaster or Drosophila yakuba); (2...... and reveal that 44.4% of them show copy number polymorphisms within a population. In conclusion, we provide a panoramic picture for the origin of new genes in Drosophila species....

  14. Intestinal stem cell response to injury: lessons from Drosophila.

    Science.gov (United States)

    Jiang, Huaqi; Tian, Aiguo; Jiang, Jin

    2016-09-01

    Many adult tissues and organs are maintained by resident stem cells that are activated in response to injury but the mechanisms that regulate stem cell activity during regeneration are still poorly understood. An emerging system to study such problem is the Drosophila adult midgut. Recent studies have identified both intrinsic factors and extrinsic niche signals that control the proliferation, self-renewal, and lineage differentiation of Drosophila adult intestinal stem cells (ISCs). These findings set up the stage to interrogate how niche signals are regulated and how they are integrated with cell-intrinsic factors to control ISC activity during normal homeostasis and regeneration. Here we review the current understanding of the mechanisms that control ISC self-renewal, proliferation, and lineage differentiation in Drosophila adult midgut with a focus on the niche signaling network that governs ISC activity in response to injury. PMID:27137186

  15. Insights on TRP Channels from In Vivo Studies in Drosophila

    Science.gov (United States)

    Minke, Baruch; Parnas, Moshe

    2007-01-01

    Transient receptor potential (TRP) channels mediate responses in a large variety of signaling mechanisms. Most studies on mammalian TRP channels rely on heterologous expression, but their relevance to in vivo tissues is not entirely clear. In contrast, Drosophila TRP and TRP-like (TRPL) channels allow direct analyses of in vivo function. In Drosophila photoreceptors, activation of TRP and TRPL is mediated via the phosphoinositide cascade, with both Ca2+ and diacylglycerol (DAG) essential for generating the light response. In tissue culture cells, TRPL channels are constitutively active, and lipid second messengers greatly facilitate this activity. Inhibition of phospholipase C (PLC) completely blocks lipid activation of TRPL, suggesting that lipid activation is mediated via PLC. In vivo studies in mutant Drosophila also reveal an acute requirement for lipid-producing enzyme, which may regulate PLC activity. Thus, PLC and its downstream second messengers, Ca2+ and DAG, constitute critical mediators of TRP/TRPL gating in vivo. PMID:16460287

  16. Identification of common excitatory motoneurons in Drosophila melanogaster larvae.

    Science.gov (United States)

    Takizawa, Eiji; Komatsu, Akira; Tsujimura, Hidenobu

    2007-05-01

    In insects, four types of motoneurons have long been known, including fast motoneurons, slow motoneurons, common inhibitory motoneurons, and DUM neurons. They innervate the same muscle and control its contraction together. Recent studies in Drosophila have suggested the existence of another type of motoneuron, the common excitatory motoneuron. Here, we found that shakB-GAL4 produced by labels this type of motoneuron in Drosophila larvae. We found that Drosophila larvae have two common excitatory motoneurons in each abdominal segment, RP2 for dorsal muscles and MNSNb/d-Is for ventral muscles. They innervate most of the internal longitudinal or oblique muscles on the dorsal or ventral body wall with type-Is terminals and use glutamate as a transmitter. Electrophysiological recording indicated that stimulation of the RP2 axon evoked excitatory junctional potential in a dorsal muscle. PMID:17867850

  17. Chemical genetics and drug screening in Drosophila cancer models

    Institute of Scientific and Technical Information of China (English)

    Mara Gladstone; Tin Tin Su

    2011-01-01

    Drug candidates often fail in preclinical and clinical testing because of reasons of efficacy and/or safety.It would be time- and cost-efficient to have screening models that reduce the rate of such false positive candidates that appear promising at first but fail later.In this regard,it would be particularly useful to have a rapid and inexpensive whole animal model that can pre-select hits from high-throughput screens but before testing in costly rodent assays.Drosophila melanogaster has emerged as a potential whole animal model for drug screening.Of particular interest have been drugs that must act in the context of multi-cellularity such as those for neurological disorders and cancer.A recent review provides a comprehensive summary of drug screening in Drosophila,but with an emphasis on neurodegenerative disorders.Here,we review Drosophila screens in the literature aimed at cancer therapeutics.

  18. RNA editing in Drosophila melanogaster: new targets and functionalconsequences

    Energy Technology Data Exchange (ETDEWEB)

    Stapleton, Mark; Carlson, Joseph W.; Celniker, Susan E.

    2006-09-05

    Adenosine deaminases that act on RNA (ADARs) catalyze the site-specific conversion of adenosine to inosine in primary mRNA transcripts. These re-coding events affect coding potential, splice-sites, and stability of mature mRNAs. ADAR is an essential gene and studies in mouse, C. elegans, and Drosophila suggest its primary function is to modify adult behavior by altering signaling components in the nervous system. By comparing the sequence of isogenic cDNAs to genomic DNA, we have identified and experimentally verified 27 new targets of Drosophila ADAR. Our analyses lead us to identify new classes of genes whose transcripts are targets of ADAR including components of the actin cytoskeleton, and genes involved in ion homeostasis and signal transduction. Our results indicate that editing in Drosophila increases the diversity of the proteome, and does so in a manner that has direct functional consequences on protein function.

  19. Caffeine Taste Signaling in Drosophila Larvae.

    Science.gov (United States)

    Apostolopoulou, Anthi A; Köhn, Saskia; Stehle, Bernhard; Lutz, Michael; Wüst, Alexander; Mazija, Lorena; Rist, Anna; Galizia, C Giovanni; Lüdke, Alja; Thum, Andreas S

    2016-01-01

    The Drosophila larva has a simple peripheral nervous system with a comparably small number of sensory neurons located externally at the head or internally along the pharynx to assess its chemical environment. It is assumed that larval taste coding occurs mainly via external organs (the dorsal, terminal, and ventral organ). However, the contribution of the internal pharyngeal sensory organs has not been explored. Here we find that larvae require a single pharyngeal gustatory receptor neuron pair called D1, which is located in the dorsal pharyngeal sensilla, in order to avoid caffeine and to associate an odor with caffeine punishment. In contrast, caffeine-driven reduction in feeding in non-choice situations does not require D1. Hence, this work provides data on taste coding via different receptor neurons, depending on the behavioral context. Furthermore, we show that the larval pharyngeal system is involved in bitter tasting. Using ectopic expressions, we show that the caffeine receptor in neuron D1 requires the function of at least four receptor genes: the putative co-receptors Gr33a, Gr66a, the putative caffeine-specific receptor Gr93a, and yet unknown additional molecular component(s). This suggests that larval taste perception is more complex than previously assumed already at the sensory level. Taste information from different sensory organs located outside at the head or inside along the pharynx of the larva is assembled to trigger taste guided behaviors. PMID:27555807

  20. Structure of full-length Drosophila cryptochrome

    Energy Technology Data Exchange (ETDEWEB)

    Zoltowski, Brian D.; Vaidya, Anand T.; Top, Deniz; Widom, Joanne; Young, Michael W.; Crane, Brian R. (Cornell); (Rockefeller)

    2011-12-15

    The cryptochrome/photolyase (CRY/PL) family of photoreceptors mediates adaptive responses to ultraviolet and blue light exposure in all kingdoms of life. Whereas PLs function predominantly in DNA repair of cyclobutane pyrimidine dimers (CPDs) and 6-4 photolesions caused by ultraviolet radiation, CRYs transduce signals important for growth, development, magnetosensitivity and circadian clocks. Despite these diverse functions, PLs/CRYs preserve a common structural fold, a dependence on flavin adenine dinucleotide (FAD) and an internal photoactivation mechanism. However, members of the CRY/PL family differ in the substrates recognized (protein or DNA), photochemical reactions catalysed and involvement of an antenna cofactor. It is largely unknown how the animal CRYs that regulate circadian rhythms act on their substrates. CRYs contain a variable carboxy-terminal tail that appends the conserved PL homology domain (PHD) and is important for function. Here, we report a 2.3-{angstrom} resolution crystal structure of Drosophila CRY with an intact C terminus. The C-terminal helix docks in the analogous groove that binds DNA substrates in PLs. Conserved Trp536 juts into the CRY catalytic centre to mimic PL recognition of DNA photolesions. The FAD anionic semiquinone found in the crystals assumes a conformation to facilitate restructuring of the tail helix. These results help reconcile the diverse functions of the CRY/PL family by demonstrating how conserved protein architecture and photochemistry can be elaborated into a range of light-driven functions.

  1. Tools for neuroanatomy and neurogenetics in Drosophila

    Energy Technology Data Exchange (ETDEWEB)

    Pfeiffer, Barret D.; Jenett, Arnim; Hammonds, Ann S.; Ngo, Teri-T B.; Misra, Sima; Murphy, Christine; Scully, Audra; Carlson, Joseph W.; Wan, Kenneth H.; Laverty, Todd R.; Mungall, Chris; Svirskas, Rob; Kadonaga, James T.; Doe, Chris Q.; Eisen, Michael B.; Celniker, Susan E.; Rubin, Gerald M.

    2008-08-11

    We demonstrate the feasibility of generating thousands of transgenic Drosophila melanogaster lines in which the expression of an exogenous gene is reproducibly directed to distinct small subsets of cells in the adult brain. We expect the expression patterns produced by the collection of 5,000 lines that we are currently generating to encompass all neurons in the brain in a variety of intersecting patterns. Overlapping 3-kb DNA fragments from the flanking noncoding and intronic regions of genes thought to have patterned expression in the adult brain were inserted into a defined genomic location by site-specific recombination. These fragments were then assayed for their ability to function as transcriptional enhancers in conjunction with a synthetic core promoter designed to work with a wide variety of enhancer types. An analysis of 44 fragments from four genes found that >80% drive expression patterns in the brain; the observed patterns were, on average, comprised of <100 cells. Our results suggest that the D. melanogaster genome contains >50,000 enhancers and that multiple enhancers drive distinct subsets of expression of a gene in each tissue and developmental stage. We expect that these lines will be valuable tools for neuroanatomy as well as for the elucidation of neuronal circuits and information flow in the fly brain.

  2. Thermal stress depletes energy reserves in Drosophila.

    Science.gov (United States)

    Klepsatel, Peter; Gáliková, Martina; Xu, Yanjun; Kühnlein, Ronald P

    2016-01-01

    Understanding how environmental temperature affects metabolic and physiological functions is of crucial importance to assess the impacts of climate change on organisms. Here, we used different laboratory strains and a wild-caught population of the fruit fly Drosophila melanogaster to examine the effect of temperature on the body energy reserves of an ectothermic organism. We found that permanent ambient temperature elevation or transient thermal stress causes significant depletion of body fat stores. Surprisingly, transient thermal stress induces a lasting "memory effect" on body fat storage, which also reduces survivorship of the flies upon food deprivation later after stress exposure. Functional analyses revealed that an intact heat-shock response is essential to protect flies from temperature-dependent body fat decline. Moreover, we found that the temperature-dependent body fat reduction is caused at least in part by apoptosis of fat body cells, which might irreversibly compromise the fat storage capacity of the flies. Altogether, our results provide evidence that thermal stress has a significant negative impact on organismal energy reserves, which in turn might affect individual fitness. PMID:27641694

  3. Host plant adaptation in Drosophila mettleri populations.

    Science.gov (United States)

    Castrezana, Sergio; Bono, Jeremy M

    2012-01-01

    The process of local adaptation creates diversity among allopatric populations, and may eventually lead to speciation. Plant-feeding insect populations that specialize on different host species provide an excellent opportunity to evaluate the causes of ecological specialization and the subsequent consequences for diversity. In this study, we used geographically separated Drosophila mettleri populations that specialize on different host cacti to examine oviposition preference for and larval performance on an array of natural and non-natural hosts (eight total). We found evidence of local adaptation in performance on saguaro cactus (Carnegiea gigantea) for populations that are typically associated with this host, and to chemically divergent prickly pear species (Opuntia spp.) in a genetically isolated population on Santa Catalina Island. Moreover, each population exhibited reduced performance on the alternative host. This finding is consistent with trade-offs associated with adaptation to these chemically divergent hosts, although we also discuss alternative explanations for this pattern. For oviposition preference, Santa Catalina Island flies were more likely to oviposit on some prickly pear species, but all populations readily laid eggs on saguaro. Experiments with non-natural hosts suggest that factors such as ecological opportunity may play a more important role than host plant chemistry in explaining the lack of natural associations with some hosts. PMID:22493678

  4. Host plant adaptation in Drosophila mettleri populations.

    Directory of Open Access Journals (Sweden)

    Sergio Castrezana

    Full Text Available The process of local adaptation creates diversity among allopatric populations, and may eventually lead to speciation. Plant-feeding insect populations that specialize on different host species provide an excellent opportunity to evaluate the causes of ecological specialization and the subsequent consequences for diversity. In this study, we used geographically separated Drosophila mettleri populations that specialize on different host cacti to examine oviposition preference for and larval performance on an array of natural and non-natural hosts (eight total. We found evidence of local adaptation in performance on saguaro cactus (Carnegiea gigantea for populations that are typically associated with this host, and to chemically divergent prickly pear species (Opuntia spp. in a genetically isolated population on Santa Catalina Island. Moreover, each population exhibited reduced performance on the alternative host. This finding is consistent with trade-offs associated with adaptation to these chemically divergent hosts, although we also discuss alternative explanations for this pattern. For oviposition preference, Santa Catalina Island flies were more likely to oviposit on some prickly pear species, but all populations readily laid eggs on saguaro. Experiments with non-natural hosts suggest that factors such as ecological opportunity may play a more important role than host plant chemistry in explaining the lack of natural associations with some hosts.

  5. Transgenerational memory effect of ageing in Drosophila.

    Science.gov (United States)

    Burns, James G; Mery, Frederic

    2010-04-01

    Children born to older parents tend to have lower intelligence and are at higher risk for disorders such as schizophrenia and autism. Such observations of ageing damage being passed on from parents to offspring are not often considered within the evolutionary theory of ageing. Here, we show the 25% memory impairment in Drosophila melanogaster offspring solely dependent on the age of the parents and also passed on to the F2 generation. Furthermore, this parental age effect was not attributed to a generalized reduction in condition of the offspring but was specific to short-term memory. We also provide evidence implicating oxidative stress as a causal factor by showing that lines selected for resistance to oxidative stress did not display a memory impairment in offspring of old parents. The identification of the parental age-related memory impairment in a model system should stimulate integration between mechanistic studies of age-related mortality risk and functional studies of parental age effects on the fitness of future generations. PMID:20149023

  6. Effects of Spaceflight on Drosophila Neural Development

    Science.gov (United States)

    Keshishian, Haig S.

    1997-01-01

    The major goal from the animal side, however, has been achieved, namely to develop Drosophila lines where we can assay individual neuromuscular endings directly without dissection. This was achieved by means of using the GAL4-UAS system, where we have succeeded in establishing stocks of flies where the key neuromuscular connections can be assayed directly in undissected larvae by means of the expression of endogenously fluorescent reporters in the specific motor endings. The green fluorescent protein (GFP) as a reporter allows scoring of neural anatomy en-masse in whole mount using fluorescent microscopy without the need for either dissection or specific labeling. Two stocks have been developed. The first, which we developed first, uses the S65T mutant form, which has a dramatically brighter expression than the native protein. This animal will use GAL4 drivers with expression under the control of the elav gene, and which will ensure expression in all neurons of the embryo and larva. The second transgenic animal we have developed is of a novel kind, and makes use of dicistronic design, so that two copies of the protein will be expressed per insert. We have also developed a tricistronic form, but this has not yet been transformed into flies, and we do not imagine that this third line will be ready in time for the flight.

  7. Mechanosensory interactions drive collective behaviour in Drosophila.

    Science.gov (United States)

    Ramdya, Pavan; Lichocki, Pawel; Cruchet, Steeve; Frisch, Lukas; Tse, Winnie; Floreano, Dario; Benton, Richard

    2015-03-12

    Collective behaviour enhances environmental sensing and decision-making in groups of animals. Experimental and theoretical investigations of schooling fish, flocking birds and human crowds have demonstrated that simple interactions between individuals can explain emergent group dynamics. These findings indicate the existence of neural circuits that support distributed behaviours, but the molecular and cellular identities of relevant sensory pathways are unknown. Here we show that Drosophila melanogaster exhibits collective responses to an aversive odour: individual flies weakly avoid the stimulus, but groups show enhanced escape reactions. Using high-resolution behavioural tracking, computational simulations, genetic perturbations, neural silencing and optogenetic activation we demonstrate that this collective odour avoidance arises from cascades of appendage touch interactions between pairs of flies. Inter-fly touch sensing and collective behaviour require the activity of distal leg mechanosensory sensilla neurons and the mechanosensory channel NOMPC. Remarkably, through these inter-fly encounters, wild-type flies can elicit avoidance behaviour in mutant animals that cannot sense the odour--a basic form of communication. Our data highlight the unexpected importance of social context in the sensory responses of a solitary species and open the door to a neural-circuit-level understanding of collective behaviour in animal groups. PMID:25533959

  8. Functional neuroanatomy of Drosophila olfactory memory formation.

    Science.gov (United States)

    Guven-Ozkan, Tugba; Davis, Ronald L

    2014-10-01

    New approaches, techniques and tools invented over the last decade and a half have revolutionized the functional dissection of neural circuitry underlying Drosophila learning. The new methodologies have been used aggressively by researchers attempting to answer three critical questions about olfactory memories formed with appetitive and aversive reinforcers: (1) Which neurons within the olfactory nervous system mediate the acquisition of memory? (2) What is the complete neural circuitry extending from the site(s) of acquisition to the site(s) controlling memory expression? (3) How is information processed across this circuit to consolidate early-forming, disruptable memories to stable, late memories? Much progress has been made and a few strong conclusions have emerged: (1) Acquisition occurs at multiple sites within the olfactory nervous system but is mediated predominantly by the γ mushroom body neurons. (2) The expression of long-term memory is completely dependent on the synaptic output of α/β mushroom body neurons. (3) Consolidation occurs, in part, through circuit interactions between mushroom body and dorsal paired medial neurons. Despite this progress, a complete and unified model that details the pathway from acquisition to memory expression remains elusive. PMID:25225297

  9. A Model of Drosophila Larva Chemotaxis.

    Directory of Open Access Journals (Sweden)

    Alex Davies

    2015-11-01

    Full Text Available Detailed observations of larval Drosophila chemotaxis have characterised the relationship between the odour gradient and the runs, head casts and turns made by the animal. We use a computational model to test whether hypothesised sensorimotor control mechanisms are sufficient to account for larval behaviour. The model combines three mechanisms based on simple transformations of the recent history of odour intensity at the head location. The first is an increased probability of terminating runs in response to gradually decreasing concentration, the second an increased probability of terminating head casts in response to rapidly increasing concentration, and the third a biasing of run directions up concentration gradients through modulation of small head casts. We show that this model can be tuned to produce behavioural statistics comparable to those reported for the larva, and that this tuning results in similar chemotaxis performance to the larva. We demonstrate that each mechanism can enable odour approach but the combination of mechanisms is most effective, and investigate how these low-level control mechanisms relate to behavioural measures such as the preference indices used to investigate larval learning behaviour in group assays.

  10. Tracking individual nanodiamonds in Drosophila melanogaster embryos

    CERN Document Server

    Simpson, David A; Kowarsky, Mark; Zeeshan, Nida F; Barson, Michael S J; Hall, Liam; Yan, Yan; Kaufmann, Stefan; Johnson, Brett C; Ohshima, Takeshi; Caruso, Frank; Scholten, Robert; Saint, Robert B; Murray, Michael J; Hollenberg, Lloyd C L

    2013-01-01

    Tracking the dynamics of fluorescent nanoparticles during embryonic development allows insights into the physical state of the embryo and, potentially, molecular processes governing developmental mechanisms. In this work, we investigate the motion of individual fluorescent nanodiamonds micro-injected into Drosophila melanogaster embryos prior to cellularisation. Fluorescence correlation spectroscopy and wide-field imaging techniques are applied to individual fluorescent nanodiamonds in blastoderm cells during stage 5 of development to a depth of ~40 \\mu m. The majority of nanodiamonds in the blastoderm cells during cellularisation exhibit free diffusion with an average diffusion coefficient of (6 $\\pm$ 3) x 10$^{-3}$ \\mu m$^2$/s, (mean $\\pm$ SD). Driven motion in the blastoderm cells was also observed with an average velocity of 0.13 $\\pm$ 0.10 \\mu m/s (mean $\\pm$ SD) \\mu m/s and an average applied force of 0.07 $\\pm$ 0.05 pN (mean $\\pm$ SD). Nanodiamonds in the periplasm between the nuclei and yolk were also...

  11. Thermal stress depletes energy reserves in Drosophila

    Science.gov (United States)

    Klepsatel, Peter; Gáliková, Martina; Xu, Yanjun; Kühnlein, Ronald P.

    2016-01-01

    Understanding how environmental temperature affects metabolic and physiological functions is of crucial importance to assess the impacts of climate change on organisms. Here, we used different laboratory strains and a wild-caught population of the fruit fly Drosophila melanogaster to examine the effect of temperature on the body energy reserves of an ectothermic organism. We found that permanent ambient temperature elevation or transient thermal stress causes significant depletion of body fat stores. Surprisingly, transient thermal stress induces a lasting “memory effect” on body fat storage, which also reduces survivorship of the flies upon food deprivation later after stress exposure. Functional analyses revealed that an intact heat-shock response is essential to protect flies from temperature-dependent body fat decline. Moreover, we found that the temperature-dependent body fat reduction is caused at least in part by apoptosis of fat body cells, which might irreversibly compromise the fat storage capacity of the flies. Altogether, our results provide evidence that thermal stress has a significant negative impact on organismal energy reserves, which in turn might affect individual fitness. PMID:27641694

  12. How food controls aggression in Drosophila.

    Directory of Open Access Journals (Sweden)

    Rod S Lim

    Full Text Available How animals use sensory information to weigh the risks vs. benefits of behavioral decisions remains poorly understood. Inter-male aggression is triggered when animals perceive both the presence of an appetitive resource, such as food or females, and of competing conspecific males. How such signals are detected and integrated to control the decision to fight is not clear. For instance, it is unclear whether food increases aggression directly, or as a secondary consequence of increased social interactions caused by attraction to food. Here we use the vinegar fly, Drosophila melanogaster, to investigate the manner by which food influences aggression. We show that food promotes aggression in flies, and that it does so independently of any effect on frequency of contact between males, increase in locomotor activity or general enhancement of social interactions. Importantly, the level of aggression depends on the absolute amount of food, rather than on its surface area or concentration. When food resources exceed a certain level, aggression is diminished, suggestive of reduced competition. Finally, we show that detection of sugar via Gr5a+ gustatory receptor neurons (GRNs is necessary for food-promoted aggression. These data demonstrate that food exerts a specific effect to promote aggression in male flies, and that this effect is mediated, at least in part, by sweet-sensing GRNs.

  13. Ferritin Assembly in Enterocytes of Drosophila melanogaster

    Directory of Open Access Journals (Sweden)

    Abraham Rosas-Arellano

    2016-02-01

    Full Text Available Ferritins are protein nanocages that accumulate inside their cavity thousands of oxidized iron atoms bound to oxygen and phosphates. Both characteristic types of eukaryotic ferritin subunits are present in secreted ferritins from insects, but here dimers between Ferritin 1 Heavy Chain Homolog (Fer1HCH and Ferritin 2 Light Chain Homolog (Fer2LCH are further stabilized by disulfide-bridge in the 24-subunit complex. We addressed ferritin assembly and iron loading in vivo using novel transgenic strains of Drosophila melanogaster. We concentrated on the intestine, where the ferritin induction process can be controlled experimentally by dietary iron manipulation. We showed that the expression pattern of Fer2LCH-Gal4 lines recapitulated iron-dependent endogenous expression of the ferritin subunits and used these lines to drive expression from UAS-mCherry-Fer2LCH transgenes. We found that the Gal4-mediated induction of mCherry-Fer2LCH subunits was too slow to effectively introduce them into newly formed ferritin complexes. Endogenous Fer2LCH and Fer1HCH assembled and stored excess dietary iron, instead. In contrast, when flies were genetically manipulated to co-express Fer2LCH and mCherry-Fer2LCH simultaneously, both subunits were incorporated with Fer1HCH in iron-loaded ferritin complexes. Our study provides fresh evidence that, in insects, ferritin assembly and iron loading in vivo are tightly regulated.

  14. Reinforcement of gametic isolation in Drosophila.

    Directory of Open Access Journals (Sweden)

    Daniel R Matute

    2010-03-01

    Full Text Available Reinforcement, a process by which natural selection increases reproductive isolation between populations, has been suggested to be an important force in the formation of new species. However, all existing cases of reinforcement involve an increase in mate discrimination between species. Here, I report the first case of reinforcement of postmating prezygotic isolation (i.e., barriers that act after mating but before fertilization in animals. On the slopes of the African island of São Tomé, Drosophila yakuba and its endemic sister species D. santomea hybridize within a well-demarcated hybrid zone. I find that D. yakuba females from within this zone, but not from outside it, show an increase in gametic isolation from males of D. santomea, an apparent result of natural selection acting to reduce maladaptive hybridization between species. To determine whether such a barrier could evolve under laboratory conditions, I exposed D. yakuba lines derived from allopatric populations to experimental sympatry with D. santomea, and found that both behavioral and gametic isolation become stronger after only four generations. Reinforcement thus appears to be the best explanation for the heightened gametic isolation seen in sympatry. This appears to be the first example in animals in which natural selection has promoted the evolution of stronger interspecific genetic barriers that act after mating but before fertilization. This suggests that many other genetic barriers between species have been increased by natural selection but have been overlooked because they are difficult to study.

  15. Collective synchronization of divisions in Drosophila development

    Science.gov (United States)

    Vergassola, Massimo

    Mitoses in the early development of most metazoans are rapid and synchronized across the entire embryo. While diffusion is too slow, in vitro experiments have shown that waves of the cell-cycle regulator Cdk1 can transfer information rapidly across hundreds of microns. However, the signaling dynamics and the physical properties of chemical waves during embryonic development remain unclear. We develop FRET biosensors for the activity of Cdk1 and the checkpoint kinase Chk1 in Drosophila embryos and exploit them to measure waves in vivo. We demonstrate that Cdk1 chemical waves control mitotic waves and that their speed is regulated by the activity of Cdk1 during the S-phase (and not mitosis). We quantify the progressive slowdown of the waves with developmental cycles and identify its underlying control mechanism by the DNA replication checkpoint through the Chk1/Wee1 pathway. The global dynamics of the mitotic signaling network illustrates a novel control principle: the S-phase activity of Cdk1 regulates the speed of the mitotic wave, while the Cdk1 positive feedback ensures an invariantly rapid onset of mitosis. Mathematical modeling captures the speed of the waves and predicts a fundamental distinction between the S-phase Cdk1 trigger waves and the mitotic phase waves, which is illustrated by embryonic ablation experiments. In collaboration with Victoria Deneke1, Anna Melbinger2, and Stefano Di Talia1 1 Department of Cell Biology, Duke University Medical Center 2 Department of Physics, University of California San Diego.

  16. Insulin signaling mediates sexual attractiveness in Drosophila.

    Directory of Open Access Journals (Sweden)

    Tsung-Han Kuo

    Full Text Available Sexually attractive characteristics are often thought to reflect an individual's condition or reproductive potential, but the underlying molecular mechanisms through which they do so are generally unknown. Insulin/insulin-like growth factor signaling (IIS is known to modulate aging, reproduction, and stress resistance in several species and to contribute to variability of these traits in natural populations. Here we show that IIS determines sexual attractiveness in Drosophila through transcriptional regulation of genes involved in the production of cuticular hydrocarbons (CHC, many of which function as pheromones. Using traditional gas chromatography/mass spectrometry (GC/MS together with newly introduced laser desorption/ionization orthogonal time-of-flight mass spectrometry (LDI-MS we establish that CHC profiles are significantly affected by genetic manipulations that target IIS. Manipulations that reduce IIS also reduce attractiveness, while females with increased IIS are significantly more attractive than wild-type animals. IIS effects on attractiveness are mediated by changes in CHC profiles. Insulin signaling influences CHC through pathways that are likely independent of dFOXO and that may involve the nutrient-sensing Target of Rapamycin (TOR pathway. These results suggest that the activity of conserved molecular regulators of longevity and reproductive output may manifest in different species as external characteristics that are perceived as honest indicators of fitness potential.

  17. Drosophila as a genetically tractable model for social insect behaviour

    Directory of Open Access Journals (Sweden)

    Alison L Camiletti

    2016-04-01

    Full Text Available The relatively simple communication, breeding and egg-making systems that govern reproduction in female Drosophila retain homology to eusocial species in which these same systems are modified to the social condition. Despite having no parental care, division of labour or subfertile caste, Drosophila may nonetheless offer a living test of certain sociobiological hypotheses framed around gene function. In this review, we make this case, and do so around the recent discovery that the non-social fly, Drosophila melanogaster, can respond to the ovary-suppressing queen pheromone of the honey bee Apis meliffera. Here, we first explain the sociobiological imperative to reconcile kin theory with molecular biology, and qualify a potential role for Drosophila. Then, we offer three applications for the fly-pheromone assay. First, the availability and accessibility of massive mutant libraries makes immediately feasible any number of open or targeted gene screens against the ovary-inhibiting response. The sheer tractability of Drosophila may therefore help to accelerate the search for genes in pheromone-responsive pathways that regulate female reproduction, including potentially any that are preserved with modification to regulate worker sterility in response to queen pheromones in eusocial taxa. Secondly, Drosophila’s powerful Gal4/UAS expression system can complement the pheromone assay by driving target gene expression into living tissue, which could be well applied to the functional testing of genes presumed to drive ovary activation or de-activation in the honey bee or other eusocial taxa. Finally, coupling Gal4 with UAS-RNAi lines can facilitate loss-of-function experiments against perception and response to the ovary inhibiting pheromone, and do so for large numbers of candidates in systematic fashion. Drosophila's utility as an adjunct to the field of insect sociobiology is not ideal, but retains surprising potential.

  18. Water Management at Australian Uranium Mines

    International Nuclear Information System (INIS)

    The Australian uranium operations are located in widely different climates, ranging from monsoonal conditions at the Ranger uranium mine in the Alligator Rivers region of the Northern Territory to semi-arid conditions in inland South Australia, where the Olympic Dam and Beverley operations are located. This paper describes the range of water management strategies that are in place or planned to deal with the varying issues facing Australia's three operations. For Olympic Dam, the strategies are focused on water conservation and reuse, and evaporation of the acidic liquor arising from the leach process. Net water usage at an in situ operation such as Beverley is considerably less than comparable underground or open pit mines as acidic leach liquor is recycled in the wellfields, and there is no water loss with tailings. However, bleeds are required to maintain control of wellfield balance and to limit the buildup of impurities. The disposal of the bleed stream into the mining aquifer via wells must be balanced carefully to ensure that it does not impact on mining or wellfield management. Water management at Ranger has evolved considerably during its almost 25-year operating life. This is particularly the case over the last 8 years with the development of pit 3 and the deposition of tailings into the mined out pit 1. These changes, which have significantly increased the catchment area from which runoff water must be collected and managed, and the recent higher-than-average rainfall combined with the further expansion of pit 3 now have the potential to move the site water balances into significant surplus. To address this risk, ERA has successfully investigated processes at the pilot plant scale that would complement the capacity of existing passive pond water treatment systems by allowing the treatment and release of pond and/or process waters. These processes, which are part of a number of strategies currently being considered for implementation at the Ranger site

  19. A requirement for Australian research: access to 'big science' facilities, a report by the Australian National Committee for crystallography

    International Nuclear Information System (INIS)

    Two types of 'Big Science' research facility - synchrotron radiation sources and intense neutron beams - are now recognised as essential resources for a wide range of research activities in chemistry, physics and biology. The cost of such facilities and the lack of a sufficiently large user base will probably preclude their construction in Australia in the foreseeable future. The needs of Australian crystallographers for access to such facilities are assessed. In relation to synchrotron radiation sources, the Committee considered only the question of access to such facilities overseas. In relation to neutron beam sources, the Committee's inquiries included not only the question of access to powerful facilities overseas but also the special problems which confront Australian crystallographers as a result of the obsolescence of the HIFAR reactor. The arguments about, and options for, funding Australian use of facilities overseas are presented. The Committee concluded there is a strong case for the purchase of a beam-line at an overseas synchrotron radiation facility and a strong, though less urgent, case for substantial Australian involvement in an overseas neutron beam facility. The Committee recommended that the Australian HIFAR reactor be refurbished in its present shell, retaining the present flux and power levels, and that in the upgrading of the neutron scattering instrumentation at HIFAR special consideration be given to including items which are sufficiently specialised to attract the international neutron scattering community

  20. How Australian and Indonesian Universities Treat Plagiarism: A Comparative Study

    Directory of Open Access Journals (Sweden)

    Bambang Yudi Cahyono

    2016-02-01

    Full Text Available This article is a part of a larger study comparing various aspects of policies on plagiarism in two university contexts. It compares policies on plagiarism in universities in Australia and Indonesia. The results of this comparative study showed that Australian and Indonesian universities treat plagiarism differently. Australian universities treat plagiarism explicitly in their university policies. In Australian universities, plagiarism is defined clearly and forms of plagiarism are explained thoroughly, policies on plagiarism are informed to all university academic members, and there are mechanisms to manage cases related to plagiarism. In contrast, not all Indonesian universities treat plagiarism directly. Some universities depend on religious morality and academic ethics in dealing with plagiarism. Accordingly, this article recommends the explicit treatment of plagiarism in Indonesian universities.

  1. Dawes Review 5: Australian Aboriginal Astronomy and Navigation

    CERN Document Server

    Norris, Ray P

    2016-01-01

    The traditional cultures of Aboriginal Australians include a significant astronomical component, perpetuated through oral tradition, ceremony, and art. This astronomical knowledge includes a deep understanding of the motion of objects in the sky, which was used for practical purposes such as constructing calendars and for navigation. There is also evidence that traditional Aboriginal Australians made careful records and measurements of cyclical phenomena, recorded unexpected phenomena such as eclipses and meteorite impacts, and could determine the cardinal points to an accuracy of a few degrees. Putative explanations of celestial phenomena appear throughout the oral record, suggesting traditional Aborig- inal Australians sought to understand the natural world around them, in the same way as modern scientists, but within their own cultural context. There is also a growing body of evidence for sophisticated navigational skills, including the use of astronomically based songlines. Songlines are effectively oral ...

  2. Paediatric Australian bat lyssavirus encephalomyelitis - sequential MRI appearances from symptom onset to death

    Energy Technology Data Exchange (ETDEWEB)

    Shetty, Umesh; Phillips, Mark; Walsh, Mark [Mater Hospital and Lady Cilento Children' s Hospital Medical Imaging Department, Brisbane, QLD (Australia); Francis, Joshua R. [Royal Darwin Hospital, Department of Paediatrics, Darwin (Australia)

    2015-10-15

    Human infection with Australian bat lyssavirus is extremely rare. Here we present the craniospinal findings in a fatal case of Australian bat lyssavirus infection in an 8-year-old child. MRI plays a very important role, not only in the diagnostic work-up of Australian bat lyssavirus infection but also in the prognostic assessment. (orig.)

  3. Enhancing Educational Performance for Remote Aboriginal Australians: What Is the Impact of Attendance on Performance?

    Science.gov (United States)

    Jorgensen, Robyn

    2012-01-01

    The educational performance of Aboriginal Australians lags behind non-Indigenous Australians with the gap increasing the longer students remain at school. The Australian government has released its Closing the Gap policy with the specific intent to redress gaps in health, education and housing, as these are seen as key indicators to life success.…

  4. Potential Effectiveness of Specific Anti-Smoking Mass Media Advertisements among Australian Indigenous Smokers

    Science.gov (United States)

    Stewart, Harold S.; Bowden, Jacqueline A.; Bayly, Megan C.; Sharplin, Greg R.; Durkin, Sarah J.; Miller, Caroline L.; Givans, Sharon E.; Warne, Charles D.; Wakefield, Melanie A.

    2011-01-01

    Aboriginal and Torres Strait Islander Australians (Indigenous Australians) have more than twice the smoking prevalence of non-Indigenous Australians. Anti-smoking campaigns have demonstrated success in the general population but little is known about their impact among Indigenous people. A total of 143 Indigenous and a comparison group of 156…

  5. Positioning the School in the Landscape: Exploring Black History with a Regional Australian Primary School

    Science.gov (United States)

    Zeegers, Margaret

    2011-01-01

    This paper deals with a project establishing an Indigenous Australian artists-in-residence program at a regional Australian primary school to foreground its Black History. Primary school students worked with Indigenous Australian story tellers, artists, dancers and musicians to explore ways in which they could examine print and non-print texts for…

  6. The Australian Geography Competition: An Overview of Participation and Results 2004-2013

    Science.gov (United States)

    Childs, Iraphne R. W.; Berg, Kathryn

    2015-01-01

    The Australian Geography Competition (AGC) was established in 1995 by the Royal Geographical Society of Queensland (RGSQ) and the Australian Geography Teachers' Association to promote the study of geography in Australian secondary schools and to reward student excellence in geographical studies. Initially focusing on students at the lower…

  7. Strategic Capacity Building for Australian Educational Research: Creating Spaces for Action

    Science.gov (United States)

    Goodyear, Peter

    2013-01-01

    This paper provides some background information about the Strategic Capacity Building for Australian Educational Research initiative: a joint program of work sponsored by the Australian Association for Research in Education and the Australian Council of Deans of Education. In addition, it offers some broader analysis of the contexts within which…

  8. Australian Information Education in the 21st Century--The Synergy among Research, Teaching and Practice

    Science.gov (United States)

    Nastasie, Daniela L.

    2012-01-01

    In 2011 a group of Australian Library and Information Science academics led by Prof. Helen Partridge conducted an investigation into the Australian Library and Information Science education in the 21st century. The project was funded by the Australian Learning and Teaching Council (ALTC) and the final report, titled "Re-conceptualising and…

  9. The making of a fusion branch in the Drosophila trachea.

    Science.gov (United States)

    Gervais, Louis; Lebreton, Gaelle; Casanova, Jordi

    2012-02-15

    Connection of epithelial tubes to generate a common network is a key step in the formation of tubular organs such as the tracheal respiratory and the vascular systems. However, it is not clear how these connecting tubes arise. Here we address this issue by studying the dorsal fusion branches in the Drosophila trachea, taking into account the morphology and contribution of each cell type on the basis of their individual labeling. Our results explain how a fusion branch forms and also illustrate the different nature of the two seamless tubes in the Drosophila trachea, generated by fusion and terminal cells respectively. PMID:22178247

  10. Getting started : an overview on raising and handling Drosophila.

    Science.gov (United States)

    Stocker, Hugo; Gallant, Peter

    2008-01-01

    Drosophila melanogaster has long been a prime model organism for developmental biologists. During their work, they have established a large collection of techniques and reagents. This in turn has made fruit flies an attractive system for many other biomedical researchers who have otherwise no background in fly biology. This review intends to help Drosophila neophytes in setting up a fly lab. It briefly introduces the biological properties of fruit flies, describes the minimal equipment required for working with flies, and offers some basic advice for maintaining fly lines and setting up and analyzing experiments. PMID:18641939

  11. Modeling dietary influences on offspring metabolic programming in Drosophila melanogaster.

    Science.gov (United States)

    Brookheart, Rita T; Duncan, Jennifer G

    2016-09-01

    The influence of nutrition on offspring metabolism has become a hot topic in recent years owing to the growing prevalence of maternal and childhood obesity. Studies in mammals have identified several factors correlating with parental and early offspring dietary influences on progeny health; however, the molecular mechanisms that underlie these factors remain undiscovered. Mammalian metabolic tissues and pathways are heavily conserved in Drosophila melanogaster, making the fly an invaluable genetic model organism for studying metabolism. In this review, we discuss the metabolic similarities between mammals and Drosophila and present evidence supporting its use as an emerging model of metabolic programming. PMID:27450801

  12. Genetic regulation of programmed cell death in Drosophila

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Programmed cell death plays an important role in maintaining homeostasis during animal development, and has been conserved in animals as different as nematodes and humans. Recent studies of Drosophila have provided valuable information toward our understanding of genetic regulation of death. Different signals trigger the novel death regulators rpr, hid, and grim, that utilize the evolutionarily conserved iap and ark genes to modulate caspase function. Subsequent removal of dying cells also appears to be accomplished by conserved mechanisms. The similarity between Drosophila and human in cell death signaling pathways illustrate the promise of fruit flies as a model system to elucidate the mechanisms underlying regulation of programmed cell death.

  13. Inference of chromosomal inversion dynamics from Pool-Seq data in natural and laboratory populations of Drosophila melanogaster.

    Science.gov (United States)

    Kapun, Martin; van Schalkwyk, Hester; McAllister, Bryant; Flatt, Thomas; Schlötterer, Christian

    2014-04-01

    Sequencing of pools of individuals (Pool-Seq) represents a reliable and cost-effective approach for estimating genome-wide SNP and transposable element insertion frequencies. However, Pool-Seq does not provide direct information on haplotypes so that, for example, obtaining inversion frequencies has not been possible until now. Here, we have developed a new set of diagnostic marker SNPs for seven cosmopolitan inversions in Drosophila melanogaster that can be used to infer inversion frequencies from Pool-Seq data. We applied our novel marker set to Pool-Seq data from an experimental evolution study and from North American and Australian latitudinal clines. In the experimental evolution data, we find evidence that positive selection has driven the frequencies of In(3R)C and In(3R)Mo to increase over time. In the clinal data, we confirm the existence of frequency clines for In(2L)t, In(3L)P and In(3R)Payne in both North America and Australia and detect a previously unknown latitudinal cline for In(3R)Mo in North America. The inversion markers developed here provide a versatile and robust tool for characterizing inversion frequencies and their dynamics in Pool-Seq data from diverse D. melanogaster populations. PMID:24372777

  14. 10th Australian conference on nuclear techniques of analysis. Proceedings

    International Nuclear Information System (INIS)

    These proceedings contains abstracts and extended abstracts of 80 lectures and posters presented at the 10th Australian conference on nuclear techniques of analysis hosted by the Australian National University in Canberra, Australia from 24-26 of November 1997. The conference was divided into sessions on the following topics : ion beam analysis and its applications; surface science; novel nuclear techniques of analysis, characterization of thin films, electronic and optoelectronic material formed by ion implantation, nanometre science and technology, plasma science and technology. A special session was dedicated to new nuclear techniques of analysis, future trends and developments. Separate abstracts were prepared for the individual presentation included in this volume

  15. 10th Australian conference on nuclear techniques of analysis. Proceedings

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-06-01

    These proceedings contains abstracts and extended abstracts of 80 lectures and posters presented at the 10th Australian conference on nuclear techniques of analysis hosted by the Australian National University in Canberra, Australia from 24-26 of November 1997. The conference was divided into sessions on the following topics : ion beam analysis and its applications; surface science; novel nuclear techniques of analysis, characterization of thin films, electronic and optoelectronic material formed by ion implantation, nanometre science and technology, plasma science and technology. A special session was dedicated to new nuclear techniques of analysis, future trends and developments. Separate abstracts were prepared for the individual presentation included in this volume.

  16. Forward contracts in electricity markets: The Australian experience

    International Nuclear Information System (INIS)

    Forward contracts play a vital role in all electricity markets, and yet the details of the market for forward contracts are often opaque. In this paper we review the existing literature on forward contracts and explore the contracting process as it operates in Australia. The paper is based on interviews with participants in Australia's National Electricity Market (NEM). The interviews were designed to understand the contracting process and the practice of risk management in the Australian energy-only pool market. This survey reveals some significant gaps between the assumptions made in the academic literature and actual practice in the Australian market place

  17. An empirical investigation of Australian Stock Exchange data

    Science.gov (United States)

    Bertram, William K.

    2004-10-01

    We present an empirical study of high frequency Australian equity data examining the behaviour of distribution tails and the existence of long memory. A method is presented allowing us to deal with Australian Stock Exchange data by splitting it into two separate data series representing an intraday and overnight component. Power-law exponents for the empirical density functions are estimated and compared with results from other studies. Using the autocorrelation and variance plots we find there to be a strong indication of long-memory type behaviour in the absolute return, volume and transaction frequency.

  18. The Australian black coal industry: productivity commission inquiry

    International Nuclear Information System (INIS)

    February 1999 saw the release of the final report of the Productivity Commission's inquiry into the Australian black coal industry. The report is a comprehensive examination of the state of play of the Australian coal industry, drawing from the Commission's own research, submissions from a range of stakeholders and the results of a benchmarking study which compared Australia's performance with world's best practice. Essentially, the report finds that recent reforms have improved productivity and some parts of the industry now perform very well. However, the report finds that further changes to federal industry awards and state legislation and regulation could significantly improve the performance of the industry overall

  19. Cerebral lateralization determines hand preferences in Australian parrots.

    Science.gov (United States)

    Brown, Culum; Magat, Maria

    2011-08-23

    Individual preference for the use of one limb over the other to explore the environment or manipulate objects is common trait among vertebrates. Here, we explore the hypothesis that limb preference is determined by the engagement of a particular cerebral hemisphere to analyse certain stimuli. We recorded the eye and foot preferences of 322 individuals from 16 species of Australian parrots while investigating potential food items. Across all species, eye preferences explained 99 per cent of the variation in foot use in Australian parrots. The vast majority of species showed significant relationships between eye and foot preferences at the population level.

  20. Design and Implementation of the Australian National Data Service

    Directory of Open Access Journals (Sweden)

    Andrew Treloar

    2009-06-01

    Full Text Available Normal 0 This paper will describe the genesis and realisation of the Australian National Data Service (ANDS. It will commence by outlining the context within which ANDS was conceived, both in the international research and Australian research support domains. It will then describe the process that brought about the ANDS vision and the principles that informed the realisation of that vision. The paper will then outline each of the four ANDS programs (Developing Frameworks, Providing Utilities, Seeding the Commons, and Building Capabilities while also discussing particular items of note about the approach ANDS is taking. The paper concludes by briefly examining related work in the UK and US.

  1. Porridge and peas: C. Stanton Hicks and Australian army rations.

    Science.gov (United States)

    Collingham, Lizzie

    2009-09-01

    In 1942 Australian troops came back from fighting the Japanese in New Guinea exhausted and malnourished. The army rations of bully beef and biscuits were insufficiently rich in vitamins to sustain men in combat in tropical conditions. The nutritionist C. Stanton Hicks was one of a vast army of scientists who worked behind the scenes to maximize the war effort. He made it his mission to improve the army diet. He set up the Australian Army Catering Corps, invented combat ration packs and tried to introduce vitamin-rich foods into the soldiers' diet. Two of his more idiosyncratic innovations were wheat porridge and Tasmanian blue peas.

  2. Australian food life style segments and elaboration likelihood differences

    DEFF Research Database (Denmark)

    Brunsø, Karen; Reid, Mike

    As the global food marketing environment becomes more competitive, the international and comparative perspective of consumers' attitudes and behaviours becomes more important for both practitioners and academics. This research employs the Food-Related Life Style (FRL) instrument in Australia...... in order to 1) determine Australian Life Style Segments and compare these with their European counterparts, and to 2) explore differences in elaboration likelihood among the Australian segments, e.g. consumers' interest and motivation to perceive product related communication. The results provide new...... insights into cross-cultural similarities and differences, into elaboration likelihood differences among consumer segments, and show how the involvement construct may be used as basis for communication development....

  3. A composite study of onset of the Australian summer monsoon

    Science.gov (United States)

    Hendon, Harry H.; Liebmann, Brant

    1990-01-01

    The circulation changes that accompany an onset (defined as the first occurrence of wet 850-mb westerly winds at Darwin, Australia) of the Australian summer monsoon are documented by a composite study for the years 1957-1987. Composites of atmospheric fields at stations in and about the Australian tropics are constructed relative to the onset data at Darwin. It is shown that the composite onset is dominated by a slow eastward migration of a deep-baroclinic convective circulation displaced south of the equator. This propagating anomaly exhibited many features of the so-called 40-50 day oscillation, including an upper level anticyclone that accompanies the convective anomaly.

  4. Course diversity within South Australian secondary schools as a factor of successful transition and retention within Australian universities

    Directory of Open Access Journals (Sweden)

    Scott Wright

    2010-07-01

    Full Text Available There has long been a disparity in the provision of curriculum within Australian secondary schools. This study aims to evaluate whether diversity within schools alters students’ university experiences. While much of the existing literature focuses on each aspect individually, this paper attempts to clarify a link between these factors by focussing on the transition process. A theoretical analysis of key concepts surrounding a web of inter-related issues, including student satisfaction, interest and motivation frames the quantitative data collection. The methodology employed consists of analysing a balanced sample of South Australian secondary schools, from an array of different locations, SES groupings and sizes, and an acknowledgement of previous studies into the first year experience within Australian Universities. The findings suggest that there is a disparity between learning areas in school curricula and an inherent link has been established with issues such as student attrition and dissatisfaction in universities.

  5. Modeling peripheral olfactory coding in Drosophila larvae.

    Directory of Open Access Journals (Sweden)

    Derek J Hoare

    Full Text Available The Drosophila larva possesses just 21 unique and identifiable pairs of olfactory sensory neurons (OSNs, enabling investigation of the contribution of individual OSN classes to the peripheral olfactory code. We combined electrophysiological and computational modeling to explore the nature of the peripheral olfactory code in situ. We recorded firing responses of 19/21 OSNs to a panel of 19 odors. This was achieved by creating larvae expressing just one functioning class of odorant receptor, and hence OSN. Odor response profiles of each OSN class were highly specific and unique. However many OSN-odor pairs yielded variable responses, some of which were statistically indistinguishable from background activity. We used these electrophysiological data, incorporating both responses and spontaneous firing activity, to develop a bayesian decoding model of olfactory processing. The model was able to accurately predict odor identity from raw OSN responses; prediction accuracy ranged from 12%-77% (mean for all odors 45.2% but was always significantly above chance (5.6%. However, there was no correlation between prediction accuracy for a given odor and the strength of responses of wild-type larvae to the same odor in a behavioral assay. We also used the model to predict the ability of the code to discriminate between pairs of odors. Some of these predictions were supported in a behavioral discrimination (masking assay but others were not. We conclude that our model of the peripheral code represents basic features of odor detection and discrimination, yielding insights into the information available to higher processing structures in the brain.

  6. Australian Institute of Sport and the Australian Paralympic Committee position statement: urinary tract infection in spinal cord injured athletes.

    Science.gov (United States)

    Compton, Stacey; Trease, Larissa; Cunningham, Corey; Hughes, David

    2015-10-01

    Patients with spinal cord injuries are at increased risk of developing symptomatic urinary tract infections. Current evidence-based knowledge regarding prevention and treatment of urinary tract infection in the spinal cord injured population is limited. There are currently no urinary tract infection prevention and management guidelines specifically targeted towards elite spinal cord injured athletes. This position statement represents a set of recommendations intended to provide clinical guidelines for sport and exercise medicine physicians and other healthcare providers for the prevention and treatment of urinary tract infection in spinal cord injured athletes. It has been endorsed by the Australian Institute of Sport (AIS) and the Australian Paralympic Committee (APC).

  7. Lower age at menarche affects survival in older Australian women: results from the Australian Longitudinal Study of Ageing

    OpenAIRE

    Giles Lynne C; Glonek Gary FV; Moore Vivienne M; Davies Michael J; Luszcz Mary A

    2010-01-01

    Abstract Background While menarche indicates the beginning of a woman's reproductive life, relatively little is known about the association between age at menarche and subsequent morbidity and mortality. We aimed to examine the effect of lower age at menarche on all-cause mortality in older Australian women over 15 years of follow-up. Methods Data were drawn from the Australian Longitudinal Study of Ageing (n = 1,031 women aged 65-103 years). We estimated the hazard ratio (HR) associated with...

  8. Genetic Analysis of the Hairy Locus in DROSOPHILA MELANOGASTER

    OpenAIRE

    Ingham, P W; Pinchin, S M; Howard, K.R.; Ish-Horowicz, D.

    1985-01-01

    Mutations of the hairy locus in Drosophila may affect both adult chaeta differentiation and embryonic segmentation. In an effort to understand this phenotypic complexity, we have analyzed 30 mutant alleles of the locus. We find that the alleles fall into four groups according to their complementation properties, suggesting a structurally complex locus in which two distinct functions share a common coding region.

  9. Interaction between the Drosophila heterochromatin proteins SUUR and HP1

    NARCIS (Netherlands)

    A.V. Pindyurin (Alexey); L.V. Boldyreva (Lidiya); V.V. Shloma (Victor); T.D. Kolesnikova (Tatiana); G.V. Pokholkova (Galina); E.N. Andreyeva (Evgeniya); E. Kozhevnikova (Elena); I.G. Ivanoschuk (Igor); E.A. Zarutskaya (Ekaterina); S.A. Demakov (Sergey); A.A. Gorchakov (Andrey); E.S. Belyaeva (Elena); I.F. Zhimulev (Igor)

    2008-01-01

    textabstractSUUR (Suppressor of Under-Replication) protein is responsible for late replication and, as a consequence, for DNA underreplication of intercalary and pericentric heterochromatin in Drosophila melanogaster polytene chromosomes. However, the mechanism by which SUUR slows down the replicati

  10. Plexins function in epithelial repair in both Drosophila and zebrafish

    Science.gov (United States)

    Yoo, Sa Kan; Pascoe, Heath G.; Pereira, Telmo; Kondo, Shu; Jacinto, Antonio; Zhang, Xuewu; Hariharan, Iswar K.

    2016-01-01

    In most multicellular organisms, homeostasis is contingent upon maintaining epithelial integrity. When unanticipated insults breach epithelial barriers, dormant programmes of tissue repair are immediately activated. However, many of the mechanisms that repair damaged epithelia remain poorly characterized. Here we describe a role for Plexin A (PlexA), a protein with particularly well-characterized roles in axonal pathfinding, in the healing of damaged epithelia in Drosophila. Semaphorins, which are PlexA ligands, also regulate tissue repair. We show that Drosophila PlexA has GAP activity for the Rap1 GTPase, which is known to regulate the stability of adherens junctions. Our observations suggest that the inhibition of Rap1 activity by PlexA in damaged Drosophila epithelia allows epithelial remodelling, thus facilitating wound repair. We also demonstrate a role for Plexin A1, a zebrafish orthologue of Drosophila PlexA, in epithelial repair in zebrafish tail fins. Thus, plexins function in epithelial wound healing in diverse taxa. PMID:27452696

  11. Bazooka mediates secondary axon morphology in Drosophila brain lineages

    Directory of Open Access Journals (Sweden)

    Hartenstein Volker

    2011-04-01

    Full Text Available Abstract In the Drosophila brain, neural lineages project bundled axon tracts into a central neuropile. Each lineage exhibits a stereotypical branching pattern and trajectory, which distinguish it from other lineages. In this study, we used a multilineage approach to explore the neural function of the Par-complex member Par3/Bazooka in vivo. Drosophila bazooka is expressed in post-mitotic neurons of the larval brain and localizes within neurons in a lineage-dependent manner. The fact that multiple GAL4 drivers have been mapped to several lineages of the Drosophila brain enables investigation of the role of Bazooka from larval to adult stages Bazooka loss-of-function (LOF clones had abnormal morphologies, including aberrant pathway choice of ventral projection neurons in the BAla1 lineage, ectopic branching in the DALv2 and BAmv1 lineages, and excess BLD5 lineage axon projections in the optic medulla. Exogenous expression of Bazooka protein in BAla1 neurons rescued defective guidance, supporting an intrinsic requirement for Bazooka in the post-mitotic neuron. Elimination of the Par-complex member Par6 recapitulated Bazooka phenotypes in some but not all lineages, suggesting that the Par complex functions in a lineage-dependent manner, and that Bazooka may act independently in some lineages. Importantly, this study highlights the potential of using a multilineage approach when studying gene function during neural development in Drosophila.

  12. Bazooka mediates secondary axon morphology in Drosophila brain lineages.

    Science.gov (United States)

    Spindler, Shana R; Hartenstein, Volker

    2011-01-01

    In the Drosophila brain, neural lineages project bundled axon tracts into a central neuropile. Each lineage exhibits a stereotypical branching pattern and trajectory, which distinguish it from other lineages. In this study, we used a multilineage approach to explore the neural function of the Par-complex member Par3/Bazooka in vivo. Drosophila bazooka is expressed in post-mitotic neurons of the larval brain and localizes within neurons in a lineage-dependent manner. The fact that multiple GAL4 drivers have been mapped to several lineages of the Drosophila brain enables investigation of the role of Bazooka from larval to adult stages Bazooka loss-of-function (LOF) clones had abnormal morphologies, including aberrant pathway choice of ventral projection neurons in the BAla1 lineage, ectopic branching in the DALv2 and BAmv1 lineages, and excess BLD5 lineage axon projections in the optic medulla. Exogenous expression of Bazooka protein in BAla1 neurons rescued defective guidance, supporting an intrinsic requirement for Bazooka in the post-mitotic neuron. Elimination of the Par-complex member Par6 recapitulated Bazooka phenotypes in some but not all lineages, suggesting that the Par complex functions in a lineage-dependent manner, and that Bazooka may act independently in some lineages. Importantly, this study highlights the potential of using a multilineage approach when studying gene function during neural development in Drosophila. PMID:21524279

  13. Fly foie gras: modeling fatty liver in Drosophila.

    Science.gov (United States)

    Arquier, Nathalie; Léopold, Pierre

    2007-02-01

    Lipids provide an essential source of metabolites and energy in normal development as well as during periods of food deprivation. A recent study in Drosophila (Gutierrez et al., 2007) reveals a novel role in regulating lipid metabolism for specialized cells called oenocytes that present striking functional similarities to mammalian hepatocytes.

  14. Dynamics of genetic rescue in inbred Drosophila melanogaster populations

    NARCIS (Netherlands)

    Bijlsma, R.; Westerhof, M. D. D.; Roekx, L. P.; Pen, I.

    2010-01-01

    Genetic rescue has been proposed as a management strategy to improve the fitness of genetically eroded populations by alleviating inbreeding depression. We studied the dynamics of genetic rescue in inbred populations of Drosophila. Using balancer chromosomes, we show that the force of heterosis that

  15. Body saccades of Drosophila consist of stereotyped banked turns

    NARCIS (Netherlands)

    Muijres, F.T.; Elzinga, M.J.; Iwasaki, N.A.; Dickinson, M.H.

    2015-01-01

    The flight pattern of many fly species consists of straight flight segments interspersed with rapid turns called body saccades, a strategy that is thought to minimize motion blur. We analyzed the body saccades of fruit flies (Drosophila hydei), using high-speed 3D videography to track body and wing

  16. Detecting novel low-abundant transcripts in Drosophila

    DEFF Research Database (Denmark)

    Lee, Sanggyu; Bao, Jingyue; Zhou, Guolin;

    2005-01-01

    Increasing evidence suggests that low-abundant transcripts may play fundamental roles in biological processes. In an attempt to estimate the prevalence of low-abundant transcripts in eukaryotic genomes, we performed a transcriptome analysis in Drosophila using the SAGE technique. We collected 244...

  17. Neurogenetics of female reproductive behaviors in Drosophila melanogaster

    NARCIS (Netherlands)

    Laturney, Meghan; Billeter, Jean-Christophe; Friedmann, T; Dunlap, JC; Goodwin, SF

    2014-01-01

    We follow an adult Drosophila melanogaster female through the major reproductive decisions she makes during her lifetime, including habitat selection, precopulatory mate choice, postcopulatory physiological changes, polyandry, and egg-laying site selection. In the process, we review the molecular an

  18. Analysis of resistance and tolerance to virus infection in Drosophila

    NARCIS (Netherlands)

    Merkling, S.H.; Rij, R.P. van

    2015-01-01

    Host defense to virus infection involves both resistance mechanisms that reduce viral burden and tolerance mechanisms that limit detrimental effects of infection. The fruit fly, Drosophila melanogaster, has emerged as a model for identifying and characterizing the genetic basis of resistance and tol

  19. Heat shock protection against cold stress of Drosophila melanogaster.

    OpenAIRE

    Burton, V; Mitchell, H K; Young, P.; Petersen, N S

    1988-01-01

    Heat shock protein synthesis can be induced during recovery from cold treatment of Drosophila melanogaster larvae. Survival of larvae after a cold treatment is dramatically improved by a mild heat shock just before the cold shock. The conditions which induce tolerance to cold are similar to those which confer tolerance to heat.

  20. Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster

    Directory of Open Access Journals (Sweden)

    Nieves Baenas

    2016-02-01

    Full Text Available We used Drosophila melanogaster as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (Raphanus sativus cv. Rambo, a Brassicaceae species rich in glucosinolates and other phytochemicals. Flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/L for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphene and raphasatin, respectively. We demonstrate that Drosophila melanogaster takes up and metabolizes isothiocyanates from radish sprouts through the detection of the metabolite sulforaphane-cysteine in fly homogenates. Moreover, we report a decrease in the glucose content of flies, an upregulation of spargel expression, the Drosophila homolog of the mammalian PPARγ-coactivator 1 α, as well as the inhibition of α-amylase and α-glucosidase in vitro. Overall, we show that the consumption of radish sprouts affects energy metabolism in Drosophila melanogaster which is reflected by lower glucose levels and an increased expression of spargel, a central player in mitochondrial biogenesis. These processes are often affected in chronic diseases associated with aging, including type II diabetes mellitus.

  1. Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster.

    Science.gov (United States)

    Baenas, Nieves; Piegholdt, Stefanie; Schloesser, Anke; Moreno, Diego A; García-Viguera, Cristina; Rimbach, Gerald; Wagner, Anika E

    2016-02-18

    We used Drosophila melanogaster as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (Raphanus sativus cv. Rambo), a Brassicaceae species rich in glucosinolates and other phytochemicals. Flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/L) for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphene and raphasatin, respectively. We demonstrate that Drosophila melanogaster takes up and metabolizes isothiocyanates from radish sprouts through the detection of the metabolite sulforaphane-cysteine in fly homogenates. Moreover, we report a decrease in the glucose content of flies, an upregulation of spargel expression, the Drosophila homolog of the mammalian PPARγ-coactivator 1 α, as well as the inhibition of α-amylase and α-glucosidase in vitro. Overall, we show that the consumption of radish sprouts affects energy metabolism in Drosophila melanogaster which is reflected by lower glucose levels and an increased expression of spargel, a central player in mitochondrial biogenesis. These processes are often affected in chronic diseases associated with aging, including type II diabetes mellitus.

  2. EGFR signaling regulates the proliferation of Drosophila adult midgut progenitors

    OpenAIRE

    Jiang, Huaqi; Edgar, Bruce A.

    2009-01-01

    In holometabolous insects, the adult appendages and internal organs form anew from larval progenitor cells during metamorphosis. As described here, the adult Drosophila midgut, including intestinal stem cells (ISCs), develops from adult midgut progenitor cells (AMPs) that proliferate during larval development in two phases. Dividing AMPs first disperse, but later proliferate within distinct islands, forming large cell clusters that eventually fuse during metamorphosis ...

  3. The olfactory circuit of the fruit fly Drosophila melanogaster

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    The olfactory circuit of the fruit fly Drosophila melanogaster has emerged in recent years as an excellent paradigm for studying the principles and mechanisms of information processing in neuronal circuits. We discuss here the organizational principles of the olfactory circuit that make it an attractive model for experimental manipulations, the lessons that have been learned, and future challenges.

  4. Muscarinic ACh Receptors Contribute to Aversive Olfactory Learning in Drosophila

    Science.gov (United States)

    Silva, Bryon; Molina-Fernández, Claudia; Ugalde, María Beatriz; Tognarelli, Eduardo I.; Angel, Cristian; Campusano, Jorge M.

    2015-01-01

    The most studied form of associative learning in Drosophila consists in pairing an odorant, the conditioned stimulus (CS), with an unconditioned stimulus (US). The timely arrival of the CS and US information to a specific Drosophila brain association region, the mushroom bodies (MB), can induce new olfactory memories. Thus, the MB is considered a coincidence detector. It has been shown that olfactory information is conveyed to the MB through cholinergic inputs that activate acetylcholine (ACh) receptors, while the US is encoded by biogenic amine (BA) systems. In recent years, we have advanced our understanding on the specific neural BA pathways and receptors involved in olfactory learning and memory. However, little information exists on the contribution of cholinergic receptors to this process. Here we evaluate for the first time the proposition that, as in mammals, muscarinic ACh receptors (mAChRs) contribute to memory formation in Drosophila. Our results show that pharmacological and genetic blockade of mAChRs in MB disrupts olfactory aversive memory in larvae. This effect is not explained by an alteration in the ability of animals to respond to odorants or to execute motor programs. These results show that mAChRs in MB contribute to generating olfactory memories in Drosophila. PMID:26380118

  5. Actin puts the squeeze on Drosophila glue secretion.

    Science.gov (United States)

    Merrifield, Christien J

    2016-02-01

    An actin filament coat promotes cargo expulsion from large exocytosing vesicles, but the mechanisms of coat formation and force generation have been poorly characterized. Elegant imaging studies of the Drosophila melanogaster salivary gland now reveal how actin and myosin are recruited, and show that myosin II forms a contractile 'cage' that facilitates exocytosis.

  6. Drosophila phosphopantothenoylcysteine synthetase is required for tissue morphogenesis during oogenesis

    NARCIS (Netherlands)

    Bosveld, Floris; Rana, Anil; Lemstra - Wierenga, Willemina; Kampinga, Harm; Sibon, Ody

    2008-01-01

    Background: Coenzyme A (CoA) is an essential metabolite, synthesized from vitamin B5 by the subsequent action of five enzymes: PANK, PPCS, PPCDC, PPAT and DPCK. Mutations in Drosophila dPPCS disrupt female fecundity and in this study we analyzed the female sterile phenotype of dPPCS mutants in detai

  7. Characterization and manipulation of fruit susceptibility to Drosophila suzukii

    Science.gov (United States)

    Drosophila suzukii (Matsumura) is an economic pest of small fruits and cherries that attacks intact ripening fruits. Host susceptibility is influenced by characteristics such as flesh firmness, penetration force of the skin, total soluble solids (TSS, also known as °Brix) and pH. Improved knowledge ...

  8. Fluctuation-Driven Neural Dynamics Reproduce Drosophila Locomotor Patterns.

    Directory of Open Access Journals (Sweden)

    Andrea Maesani

    2015-11-01

    Full Text Available The neural mechanisms determining the timing of even simple actions, such as when to walk or rest, are largely mysterious. One intriguing, but untested, hypothesis posits a role for ongoing activity fluctuations in neurons of central action selection circuits that drive animal behavior from moment to moment. To examine how fluctuating activity can contribute to action timing, we paired high-resolution measurements of freely walking Drosophila melanogaster with data-driven neural network modeling and dynamical systems analysis. We generated fluctuation-driven network models whose outputs-locomotor bouts-matched those measured from sensory-deprived Drosophila. From these models, we identified those that could also reproduce a second, unrelated dataset: the complex time-course of odor-evoked walking for genetically diverse Drosophila strains. Dynamical models that best reproduced both Drosophila basal and odor-evoked locomotor patterns exhibited specific characteristics. First, ongoing fluctuations were required. In a stochastic resonance-like manner, these fluctuations allowed neural activity to escape stable equilibria and to exceed a threshold for locomotion. Second, odor-induced shifts of equilibria in these models caused a depression in locomotor frequency following olfactory stimulation. Our models predict that activity fluctuations in action selection circuits cause behavioral output to more closely match sensory drive and may therefore enhance navigation in complex sensory environments. Together these data reveal how simple neural dynamics, when coupled with activity fluctuations, can give rise to complex patterns of animal behavior.

  9. Integrating computational biology and forward genetics in Drosophila.

    Directory of Open Access Journals (Sweden)

    Stein Aerts

    2009-01-01

    Full Text Available Genetic screens are powerful methods for the discovery of gene-phenotype associations. However, a systems biology approach to genetics must leverage the massive amount of "omics" data to enhance the power and speed of functional gene discovery in vivo. Thus far, few computational methods for gene function prediction have been rigorously tested for their performance on a genome-wide scale in vivo. In this work, we demonstrate that integrating genome-wide computational gene prioritization with large-scale genetic screening is a powerful tool for functional gene discovery. To discover genes involved in neural development in Drosophila, we extend our strategy for the prioritization of human candidate disease genes to functional prioritization in Drosophila. We then integrate this prioritization strategy with a large-scale genetic screen for interactors of the proneural transcription factor Atonal using genomic deficiencies and mutant and RNAi collections. Using the prioritized genes validated in our genetic screen, we describe a novel genetic interaction network for Atonal. Lastly, we prioritize the whole Drosophila genome and identify candidate gene associations for ten receptor-signaling pathways. This novel database of prioritized pathway candidates, as well as a web application for functional prioritization in Drosophila, called Endeavour-HighFly, and the Atonal network, are publicly available resources. A systems genetics approach that combines the power of computational predictions with in vivo genetic screens strongly enhances the process of gene function and gene-gene association discovery.

  10. The metabolic profile of long-lived Drosophila melanogaster

    DEFF Research Database (Denmark)

    Sarup, Pernille Merete; Pedersen, Simon Metz; Nielsen, Niels Christian;

    2012-01-01

    We investigated the age-related changes in the metabolic profile of male Drosophila melanogaster and compared the metabolic profile of flies selected for increased longevity to that of control flies of equal age. We found clear differences in metabolite composition between selection regimes and a...

  11. Tandem Duplications and the Limits of Natural Selection in Drosophila yakuba and Drosophila simulans.

    Directory of Open Access Journals (Sweden)

    Rebekah L Rogers

    Full Text Available Tandem duplications are an essential source of genetic novelty, and their variation in natural populations is expected to influence adaptive walks. Here, we describe evolutionary impacts of recently-derived, segregating tandem duplications in Drosophila yakuba and Drosophila simulans. We observe an excess of duplicated genes involved in defense against pathogens, insecticide resistance, chorion development, cuticular peptides, and lipases or endopeptidases associated with the accessory glands across both species. The observed agreement is greater than expectations on chance alone, suggesting large amounts of convergence across functional categories. We document evidence of widespread selection on the D. simulans X, suggesting adaptation through duplication is common on the X. Despite the evidence for positive selection, duplicates display an excess of low frequency variants consistent with largely detrimental impacts, limiting the variation that can effectively facilitate adaptation. Standing variation for tandem duplications spans less than 25% of the genome in D. yakuba and D. simulans, indicating that evolution will be strictly limited by mutation, even in organisms with large population sizes. Effective whole gene duplication rates are low at 1.17 × 10-9 per gene per generation in D. yakuba and 6.03 × 10-10 per gene per generation in D. simulans, suggesting long wait times for new mutations on the order of thousands of years for the establishment of sweeps. Hence, in cases where adaptation depends on individual tandem duplications, evolution will be severely limited by mutation. We observe low levels of parallel recruitment of the same duplicated gene in different species, suggesting that the span of standing variation will define evolutionary outcomes in spite of convergence across gene ontologies consistent with rapidly evolving phenotypes.

  12. The Directive Communication of Australian Primary School Principals

    Science.gov (United States)

    De Nobile, John

    2015-01-01

    Directive communication is a key leadership practise in schools. However, very little direct attention has been given to this important feature of the school communication system. The purpose of the research reported here was to produce a richer description of directive communication in the context of Australian primary schools, and in so doing,…

  13. Human Rights and History Education: An Australian Study

    Science.gov (United States)

    Burridge Nina; Buchanan, John; Chodkiewicz, Andrew

    2014-01-01

    The place of education for and about human rights within the school curriculum remains contested and this paper reports on the first national cross-sectoral investigation of its place in Australian curricula and more specifically in national and state History curriculum documents. Opportunities for the inclusion of human rights based studies were…

  14. GLBTIQ Teachers in Australian Education Policy: Protections, Suspicions, and Restrictions

    Science.gov (United States)

    Jones, Tiffany; Gray, Emily; Harris, Anne

    2014-01-01

    Recognition of human rights on the basis of sexual orientation, gender identity and intersex status by the United Nations has led to the development of new policies concerning homophobia and transphobia in educational contexts. This paper examines new Australian education policies impacting gay, lesbian, bisexual, transgender, intersex and queer…

  15. Is Mixed Methods Research Used in Australian Career Development Research?

    Science.gov (United States)

    Cameron, Roslyn

    2010-01-01

    Mixed methods research has become a substantive and growing methodological force that is growing in popularity within the human and social sciences. This article reports the findings of a study that has systematically reviewed articles from the "Australian Journal of Career Development" from 2004 to 2009. The aim of the study was to provide a…

  16. Sixth Australian conference on nuclear techniques of analysis: proceedings

    International Nuclear Information System (INIS)

    These proceedings contain the abstracts of 77 lectures. The topics focus on instrumentation, nuclear techniques and their applications for material science, surfaces, archaeometry, art, geological, environmental and biomedical studies. An outline of the Australian facilities available for research purposes is also provided. Separate abstracts were prepared for the individual papers in this volume

  17. Aligning IT and Business Strategy: An Australian University Case Study

    Science.gov (United States)

    Dent, Alan

    2015-01-01

    Alignment with business objectives is considered to be an essential outcome of information technology (IT) strategic planning. This case study examines the process of creating an IT strategy for an Australian university using an industry standard methodology. The degree of alignment is determined by comparing the strategic priorities supported by…

  18. Resistance to L2 Pragmatics in the Australian ESL Context

    Science.gov (United States)

    Davis, John McE.

    2007-01-01

    The study examines how learner biases toward a particular national type of English affect interlanguage pragmatics. Specifically, this study assesses the degree to which Korean ESL (English as a second language) students' preferences for North American English influence their willingness to use Australian-English routines while studying in…

  19. Controlling Non-Point Source Pollution in Australian Agricultural Systems

    Institute of Scientific and Technical Information of China (English)

    C. GOURLEY; A. RIDLEY

    2005-01-01

    The Australian farming sector is continuing to intensify, particularly within 300 km of the east and southern coastlines.In the future there will be fewer and larger farms, which will use more fertilizer, support more stock, grow more monoculture crops, and utilise more marginal soils. This is likely to increase the major environmental impacts of soil degradation, salt,nutrient and sediment contamination of waterways, and greenhouse gas emissions. Australian national water policy continues to focus on land, stream and groundwater salinity issues, although there is now a greater recognition of the importance of nitrogen and phosphorus losses from agriculture. The general philosophy of policy for dealing with nonpoint source pollution has been towards a voluntary rather than regulatory approach, with state and national governments supporting a range of programs to encourage sustainable agricultural practices. A catchment (watershed) based approach,through the use of integrated catchment management plans, is the primary way that non-point source pollution is addressed at the farm and local level. At an industry level, cotton, grains, meat, sugarcane and dairy amongst others, as well as the Australian fertilizer industry, have responded to non-point source issues by investing in research and development, and developing codes of practice aimed at abating these environmental impacts. Understanding the economic, social, political and cultural contexts of farming as well as the environmental impacts of agriculture are very important in determining the appropriateness of policy responses for Australian farming systems.

  20. Essential books and journals in clinical neuropsychology: an Australian perspective.

    Science.gov (United States)

    Sullivan, Karen A; Ryan, Joseph J

    2004-04-01

    To assist in the identification of key professional resources for neuropsychologists, 214 Australian clinical neuropsychologists were asked to identify "essential" reference materials. Fifty members of the Australian College of Clinical Neuropsychologists returned useable surveys. Forty-three respondents provided information about which clinical neuropsychology books and journals they considered essential and why. Results showed 15 books, including 3 Australian books, and 31 journals were included in the essential reading list of at least 10% of this sample. Compared to similar previous surveys (predominantly conducted overseas and mostly over 10 years ago), the results of this survey suggest that, Australian neuropsychologists have similar views about the top ranking books and journals in clinical neuropsychology as their overseas counterparts. An exception to this general trend may be the status of reference books containing norms, which appear to be growing in perceived importance. Importantly, the results of this survey may be used to help practitioners identify key professional resources in the area of clinical neuropsychology. PMID:15202548

  1. Food irradiation: an inquiry by the Australian Consumers' Association

    International Nuclear Information System (INIS)

    The Australian Consumers' Association's Inquiry into Food Irradiation was undertaken at the request of the Commonwealth Minister of Health, Dr N Blewett. The terms of reference of the Inquiry covered the implications of food irradiation in terms of consumer health, the environment, and the cost to the consumer

  2. Career Decision-Making: What Matters to Indigenous Australians?

    Science.gov (United States)

    Helme, Sue

    2010-01-01

    This article brings together and discusses three research projects that examined the vocational education and career-decision making of Indigenous Australians. These studies focused on the experiences of Indigenous people themselves, in order to provide an Indigenous perspective on vocational and career development. Four main barriers that limit…

  3. A Ten Year Citation Analysis of Major Australian Research Institutions

    Science.gov (United States)

    Batterham, Robin J.

    2011-01-01

    The introduction of the Excellence in Research for Australia scheme has heightened debate amongst research institutions over the use of metrics such as citations, especially given the ready availability of citation data. An analysis is presented of the citation performance of nine Australian universities and the Commonwealth Scientific, Industrial…

  4. Economies of Scale and Scope in Australian Higher Education

    Science.gov (United States)

    Worthington, A. C.; Higgs, H.

    2011-01-01

    This paper estimates economies of scale and scope for 36 Australian universities using a multiple-input, multiple-output cost function over the period 1998-2006. The three inputs included in the analysis are full-time equivalent academic and non-academic staff and physical capital. The five outputs are undergraduate, postgraduate and PhD…

  5. Ideologies of Religion and Diversity in Australian Public Schools

    Science.gov (United States)

    Byrne, Catherine

    2012-01-01

    In many multicultural democracies, education has a Christian history. However, teaching religion has ideological variation. Progressives teach about many religions, while conservatives favor (often exclusive) instruction into one tradition. Australian secular education controversially prioritizes faith-forming instruction (mostly Christian). In…

  6. Australian Radiation Protection and Nuclear Safety Regulations 1999

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-11-01

    This document contains statutory rules made under the Australian Radiation and Nuclear Safety Act 1998 defining how specified standards to be observed, practices and procedures to be followed and measures to be taken by controlled persons in relation to activities relating to controlled facilities, as well as in relation to dealings with controlled apparatus or controlled material

  7. How Law Manifests Itself in Australian Aboriginal Art

    NARCIS (Netherlands)

    A.T.M. Schreiner

    2013-01-01

    The article How Law Manifests Itself in Australian Aboriginal Art will discuss two events at the Aboriginal Art Museum Utrecht from the perspective of a meeting between two artistic and legal cultures. The first event, on the art and law of the Spinifex people, will prove to be of a private law natu

  8. Australian Students' Perceptions of Racial Attitudes in the United States.

    Science.gov (United States)

    Evans, Anna M.; Evans, Virden; Evans, Adeline L.

    1998-01-01

    This survey of the perceptions of Australian high school students toward racism in America indicates that a majority knew little about cultural diversity; had various cultural backgrounds; were influenced more by television than other forms of media; and believed African Americans do not have equal access to education, equal opportunity to…

  9. [The Australian nurses in France during the Great War].

    Science.gov (United States)

    Smith, Neil

    2014-06-01

    Australia was dragged into the First World War due to its status as a dominion of the British Empire. High numbers of nurses, both civilian and military, served during this conflict, notably in France, reflecting the surge of support and sympathy felt by Australians for the country.

  10. Educating Refugee-Background Students in Australian Schools and Universities

    Science.gov (United States)

    Naidoo, Loshini

    2015-01-01

    The Australian federal government recently set a challenging national aim: By 2020, 20% of higher education enrolment at the undergraduate level will include students from low socioeconomic backgrounds. Although refugee-background students are often members of the targeted sub-population, their educational journeys frequently require special forms…

  11. An Aboriginal Australian Genome Reveals Separate Human Dispersals into Asia

    DEFF Research Database (Denmark)

    Rasmussen, Morten; Guo, Xiaosen; Wang, Yong;

    2011-01-01

    We present an Aboriginal Australian genomic sequence obtained from a 100-year-old lock of hair donated by an Aboriginal man from southern Western Australia in the early 20th century. We detect no evidence of European admixture and estimate contamination levels to be below 0.5%. We show that Abori...

  12. Developing Sustainable Language Learning Pathways: An Australian Initiative

    Science.gov (United States)

    Chesterton, Paul; Steigler-Peters, Susi; Moran, Wendy; Piccioli, Maria Teresa

    2004-01-01

    This paper reports some key findings from an external evaluation of an innovative programme for foreign and heritage languages in the Australian state of New South Wales (NSW). The programme, entitled the Languages Continuity Initiative (LCI), was funded by the NSW Department of Education and Training and involved over 200 schools in its initial…

  13. Australian Mining and Petroleum Law Association yearbook 1998

    Energy Technology Data Exchange (ETDEWEB)

    Rowan, B. [ed.

    1998-12-31

    Papers are presented under the following headings: state interest - Gawler Craton; constitutional law; confidential information; long term gas contracts - past, present and future; resource project financing; contracting for electricity; tax on transactions; joint venture issues; recent developments; climate change and the Kyoto Protocol - emissions trading and Australian resource industries; native title and cultural heritage and corporate governance. One paper has been abstracted separately.

  14. Social Exclusion of Australian Childless Women in Their Reproductive Years

    Directory of Open Access Journals (Sweden)

    Beth Turnbull

    2016-02-01

    Full Text Available Research suggests Australian childless women are at risk of pronatalism-driven social exclusion. This exploratory, mixed methods, cross-sectional study described and explored the social exclusion of Australian childless women aged 25 to 44 years, and asked: what are the nature and extent of social exclusion of childless women; and do the nature and extent of exclusion vary for different types of childless women? A total of 776 childless female Australian residents aged 25 to 44 years completed a self-administered questionnaire. Quantitative data were collected on childlessness types, indicators of exclusion and perceived stigmatisation and exclusion due to being childless. Data were analysed using descriptive statistics, One Way ANOVAs and Kruskal Wallis Analysis of Ranks. Qualitative data on childless women’s experiences were inductively thematically analysed. Findings suggest societal-level pronatalism drives exclusion of Australian childless women. While exclusion occurs in all domains of life, childless women experience more exclusion, and perceive more exclusion due to being childless, in the social and civic domains than the service and economic domains. Circumstantially and involuntarily childless women, followed by voluntarily childless women, perceive more exclusion due to being childless than undecided and future childed women. Experiences are influenced by the nature of women’s ‘deviance’ from pronatalism.

  15. Dimensions and Domains of Organisational Effectiveness in Australian Higher Education.

    Science.gov (United States)

    Lysons, Art

    1990-01-01

    Research in Australian higher education testing one theory of dimensions of organizational effectiveness is reviewed, and two theories are analyzed to develop a more comprehensive set of dimensions. The existing taxonomy is further defined, and the implications relating to recent structural adjustments in the higher education system are discussed.…

  16. Australian Curriculum Reform II: Health and Physical Education

    Science.gov (United States)

    Lynch, Timothy

    2014-01-01

    It is implied by governing organizations that Australia is presently experiencing its first national curriculum reform, when as the title suggests it is the second. However, until now Australian states and territories have been responsible for the education curriculum delivered within schools. The present national curriculum reform promises one…

  17. Rethinking Leadership in the Academy: An Australian Case

    Science.gov (United States)

    Bianchini, Stefano; Maxwell, Tudor; Dovey, Ken

    2014-01-01

    As with higher education institutions in other Organisation for Economic Cooperation and Development countries, Australian universities are facing significant challenges. One particular challenge is that of the declining quality of the teaching and learning experience within the academy. This paper describes an attempt to sustain the quality of a…

  18. Experiences of Australian School Staff in Addressing Student Cannabis Use

    Science.gov (United States)

    Gates, Peter J.; Norberg, Melissa M.; Dillon, Paul; Manocha, Ramesh

    2013-01-01

    Cannabis is the most frequently used illicit drug by Australian secondary school students yet there is scant research investigating school staff responses to student cannabis use. As such, this study surveyed 1,692 school staff who attended "Generation Next" seminars throughout Australia. The self-complete survey identified that the…

  19. Benefits of e-Learning Benchmarks: Australian Case Studies

    Science.gov (United States)

    Choy, Sarojni

    2007-01-01

    In 2004 the Australian Flexible Learning Framework developed a suite of quantitative and qualitative indicators on the uptake, use and impact of e-learning in the Vocational Education and Training (VET) sector. These indicators were used to design items for a survey to gather quantitative data for benchmarking. A series of four surveys gathered…

  20. Australian Studies in Europe and the Omnipresent Elephant

    DEFF Research Database (Denmark)

    Jensen, Lars

    2009-01-01

    Australian Studies has to undergo a transformation from its overtly literary focus to a more interdisciplinary approach, if it is to have a viable future. Rather than seeing this as a moment of unproductive stalemate, the article argues for the advantages in developing such a new focus. Also the ...

  1. Holiday and School-Term Sleep Patterns of Australian Adolescents

    Science.gov (United States)

    Warner, Suzanne; Murray, Greg; Meyer, Denny

    2008-01-01

    The holiday and school-term sleep patterns of 310 Australian senior school students were surveyed in a longitudinal study, along with self-reported sleep quality, mood, daytime functioning, grades and circadian preference. Evidence was found that with the impact of school schedule, students accrued a significant sleep debt, obtaining insufficient…

  2. Pathways for Indigenous Education in the Australian Curriculum Framework

    Science.gov (United States)

    Nakata, Martin

    2011-01-01

    This article reflects on pathways for Indigenous education in the developing agenda of the Australian Curriculum, the cross-curriculum priorities, the general capability area of intercultural understanding, and the positioning of Indigenous learners within the diversity of learners with English as an additional language or dialect (EALD).

  3. Australian Aboriginal Deaf People and Aboriginal Sign Language

    Science.gov (United States)

    Power, Des

    2013-01-01

    Many Australian Aboriginal people use a sign language ("hand talk") that mirrors their local spoken language and is used both in culturally appropriate settings when speech is taboo or counterindicated and for community communication. The characteristics of these languages are described, and early European settlers' reports of deaf Aboriginal…

  4. The Work-Related Attitudes of Australian Accounting Academics

    Science.gov (United States)

    Pop-Vasileva, Aleksandra; Baird, Kevin; Blair, Bill

    2014-01-01

    This study examines the work-related attitudes of Australian accounting academics. A survey of 350 academics provides an insight into the specific organisational and institutional factors associated with the dissatisfaction, stress levels, and propensity to remain of academics. Of particular concern is the lower level of satisfaction and…

  5. Australian Primary In-Service Teachers' Conceptions of Geography

    Science.gov (United States)

    Preston, Lou

    2015-01-01

    This paper reports on the second part of a two pronged qualitative investigation that examines the ways in which Australian primary teachers conceptualise geography and geography teaching. In the first part of the project, 47 pre-service primary teachers were surveyed. In this paper, I draw on interviews with six in-service primary teachers to…

  6. Examining Education Qualifications for Australian Vocational Education Practitioners

    Science.gov (United States)

    Williams, Kim Marianne

    2010-01-01

    This paper addresses the practice of professional development within the vocational education and training (VET) arena. The study objective was to explore appropriate education qualifications for Australian VET practitioners in the tourism and hospitality sector required for effective teaching practice. The study was conducted utilising…

  7. Non-Technical Skill Gaps in Australian Business Graduates

    Science.gov (United States)

    Jackson, Denise; Chapman, Elaine

    2012-01-01

    Purpose: The need for "job-ready" graduates has catalysed the development of non-technical skills in higher education institutions worldwide. Continued criticism of business school outcomes has provoked this examination of non-technical skill deficiencies in Australian business graduates. The purpose of this paper is to compare findings with…

  8. The Rise and Fall of the Australian DBA

    Science.gov (United States)

    Kortt, Michael A.; Pervan, Simon J; Hogan, Owen

    2016-01-01

    Purpose: The purpose of this paper is to identify and discuss the drivers behind the rise and fall of the Australian Doctor of Business Administration (DBA) and to assess its future. Design/methodology/approach: Data covering the period 1993-2013 was sourced from the Commonwealth Department of Education and Training to provide a comprehensive…

  9. Lead poisoning in Australian fruit bats (Pteropus poliocephalus)

    Energy Technology Data Exchange (ETDEWEB)

    Zook, B.C.; Sauer, R.M.; Garner, F.M.

    1970-09-01

    Lead poisoning was diagnosed in 3 Australian fruit bats. Diagnoses were indicated by the finding of large acid-fast intranuclear inclusion bodies in renal and hepatic cells, and toxic amounts of lead in tissues. The source of lead was believed to be peeling leaded paint from the walls of the bats' cage.

  10. Glacial to Holocene swings of the Australian-Indonesian monsoon

    NARCIS (Netherlands)

    Mohtadi, M.; Oppo, D.W.; Steinke, S.; Stuut, J.B.W.; De Pol-Holz, R.; Hebbeln, D.; Lückge, A.; Stuut, J.B.W.; De-Pol-Holz, R.

    2011-01-01

    The Australian-Indonesian monsoon is an important component of the climate system in the tropical Indo-Pacific region(1). However, its past variability, relation with northern and southern high-latitude climate and connection to the other Asian monsoon systems are poorly understood. Here we present

  11. How Law Manifests Itself in Australian Aboriginal Art

    NARCIS (Netherlands)

    A.T.M. Schreiner (Agnes)

    2013-01-01

    markdownabstract__Abstract__ The article How Law Manifests Itself in Australian Aboriginal Art will discuss two events at the Aboriginal Art Museum Utrecht from the perspective of a meeting between two artistic and legal cultures. The first event, on the art and law of the Spinifex people, will pro

  12. Asian Students: Their Experiences and Perceptions of Australian Universities.

    Science.gov (United States)

    Wilkinson, John W.; And Others

    1996-01-01

    A survey of 111 Asian students attending three South Australian universities found varied reasons for studying in Australia, four distinct student segments, but no dominant selection criteria. About one-quarter experienced communication problems, homesickness, or loneliness. Study-related difficulties varied, related to segment. A large minority…

  13. Implementing Cooperative Learning in Australian Primary Schools: Generalist Teachers' Perspectives

    Science.gov (United States)

    Hennessey, Angela; Dionigi, Rylee A.

    2013-01-01

    To implement cooperative learning successfully in practice, teachers require knowledge of cooperative learning, its features and terms, and how it functions in classrooms. This qualitative study examined 12 Australian generalist primary teachers', understandings of cooperative learning and perceived factors affecting its implementation. Using…

  14. Models of Reference Services in Australian Academic Libraries

    Science.gov (United States)

    Burke, Liz

    2008-01-01

    This article reports on a project which was undertaken in 2006 to investigate the current modes and methods for delivering reference services in Australian academic libraries. The project included a literature review to assist in providing a definition of reference services as well as a snapshot of statistics showing staff and patron numbers from…

  15. Species of Pleistodontes from the Australian continent (Hymenoptera, Agaonidae)

    NARCIS (Netherlands)

    Wiebes, J.T.

    1990-01-01

    Review of the Australian species of Pleistodontes Saunders (Hymenoptera Chalcidoidea, Agaonidae: fig insects), with description of new species Pleistodontes cuneatus (from Ficus leucotricha Miq.) and P. proximus (from F. platypoda A. Cunn. ex Miq.), both collected in E. Kimberley, W. Australia, and

  16. Motivational Postures and Compliance with Environmental Law in Australian Agriculture

    Science.gov (United States)

    Bartel, Robyn; Barclay, Elaine

    2011-01-01

    Motivational posture theory is applied and extended to the context of Australian agriculture and environmental regulation. Regulatory failure in this area has been observed but little was known of the compliance attitudes and behaviours of farmers prior to this study. Agriculture covers over 60% of Australia's land surface so this information is…

  17. Student Engagement and Departure Intention: An Australian University Perspective

    Science.gov (United States)

    Jackling, Beverley; Natoli, Riccardo

    2011-01-01

    This study addresses the departure intentions of an Australian university business student cohort that is characterised by high levels of diversity in pre-entry attributes. The study investigates the level of student engagement using the academic and social integration components of the Student Engagement Questionnaire (SEQ) based on Tinto's model…

  18. Australian Vice-Chancellors: Incumbency Patterns in an Emerging System.

    Science.gov (United States)

    Sloper, David

    1987-01-01

    This paper presents baseline data about the incumbency of Australian vice-chancellors who held appointments from 1963 to 1983. Findings are that the average length of the incumbency has declined from 13.6 years to 8.0 years and that the subsequent period will witness even higher rates of turnover. (TE)

  19. Australian Educational Institutions International Markets: A Correspondence Analysis

    Science.gov (United States)

    Mazzarol, Timothy W.; Soutar, Geoffrey N.

    2008-01-01

    Purpose: The global market for international students have become highly competitive and many institutions, particularly higher education institutions, rely heavily on fee income from overseas students. This study aims to examine the countries from which Australian education institutions draw such students and used this information to better…

  20. Entrepreneurship and Educational Leadership Development: Canadian and Australian Perspectives

    Science.gov (United States)

    Webber, Charles F.; Scott, Shelleyann

    2008-01-01

    This article reports the entrepreneurial activities of two university faculties, one Canadian and the other Australian, that were designed to meet the educational needs of students and to garner the resources necessary for program delivery. A conceptual framework for educational entrepreneurship, containing six dimensions, is proposed. The…