Sample records for aurin

  1. Ascertainment of aurin ear drops antimicrobial and healing effect in dog otitis

    Directory of Open Access Journals (Sweden)

    Borisov Ivan


    Full Text Available Microbiological and clinical examinations have been made, related to the effect of Aurin ear drops - the solution "Primavet - Sofia" on dogs with acute and chronic conditions of external and media otitis. The pharmaceutical compatibility of the active substances and solution stability were examined. The inhibitory effects in vitro of Acidum boricum, Acidwn salicylicum Ethanolum and Aurin - solution on laboratory referents and clinically isolated strains of microorganisms were studied. The dosage and manner of application were specified and was considered the effect on different clinical forms of otitis were specified. In the course of the one-year examination period, it was ascertained that Aurin-solution possesses the necessary stability. The active Aurin-solution substances (Acidum boricum Acidum salicylicum, Novocainum secure optimal composition of supporting substances, and high inhibitory activity in vitro on microbial strains in the examined agents of dog otitis. A dosage of 10 to 20 drops and touching the earconch with 5-10 drops twice a day, in the course of 5-7 days, has local antiseptic, anti-inflammatory, antimycotic and pain soothing effects. The healing effect of Aurin is due to selective antibacterial and antimycotic effects of the active substances used. The effectiveness against otitis is due to synergism of the effects of active and supporting substances. The obtained clinical results present grounds for recommending Aurin - ear drops as an effective method of healing dog otitis.

  2. Aurin tricarboxylic acid self-protects by inhibiting aberrant complement activation at the C3 convertase and C9 binding stages. (United States)

    Lee, Moonhee; Guo, Jian-Ping; McGeer, Edith G; McGeer, Patrick L


    Aberrant complement activation is known to exacerbate the pathology in a spectrum of degenerative diseases of aging. We previously reported that aurin tricarboxylic acid (ATA) is an orally effective agent which prevents formation of the membrane attack complex of complement. It inhibits C9 attachment to tissue bound C5b678 and thus prevents bystander lysis of host cells. In this study, we investigated the effects of ATA on the alternative complement pathway. We found that ATA prevented cleavage of the tissue bound properdin-C3b-Factor B complex into the active C3 convertase enzyme properdin-C3b-Factor Bb. This inhibition was reversed by adding Factor D to the serum. Using enzyme-linked immunosorbent type assays, we established that ATA binds directly to Factor D and C9 but not to properdin or other complement proteins. We conclude that ATA, by inhibiting at two stages of the alternative pathway, might be a particularly effective therapeutic agent in conditions such as macular degeneration, paroxysmal nocturnal hemoglobinemia, and rheumatoid arthritis, in which activation of the alternative complement pathway initiates self damage.



    Iso-Oja, Mikko; Katajisto, Jarno


    Opinnäytetyön tarkoituksena oli tehdä monipuolinen työ, jossa tulisi esille laajasti eri osa-alueita. Osaan meillä löytyi valmiina hyvä tuntemus mutta joistain osa-alueista emme tienneet paljoa. Tarkoituksena oli myös, että saisimme tehtyä työn josta olisi oikeasti jotain hyötyä. Halusimme työstä tarkoituksella monialaisen, jotta uuden oppiminen kuuluisi myös työhön. Opinnäytetyön tarkoituksena on tehdä toiminnallisesti omavarainen mahdollisimman automaattinen sääasema, joka toimii aurin...

  4. Inhibition of aberrant complement activation by a dimer of acetylsalicylic acid. (United States)

    Lee, Moonhee; Wathier, Matthew; Love, Jennifer A; McGeer, Edith; McGeer, Patrick L


    We here report synthesis for the first time of the acetyl salicylic acid dimer 5,5'-methylenebis(2-acetoxybenzoic acid) (DAS). DAS inhibits aberrant complement activation by selectively blocking factor D of the alternative complement pathway and C9 of the membrane attack complex. We have previously identified aurin tricarboxylic and its oligomers as promising agents in this regard. DAS is much more potent, inhibiting erythrocyte hemolysis by complement-activated serum with an IC50 in the 100-170 nanomolar range. There are numerous conditions where self-damage from the complement system has been implicated in the pathology, including such chronic degenerative diseases of aging as Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, and age-related macular degeneration. Consequently, there is a high priority for the discovery and development of agents that can successfully treat such conditions. DAS holds considerable promise for being such an agent.

  5. Specific interaction of aurintricarboxylic acid with the human immunodeficiency virus/CD4 cell receptor

    Energy Technology Data Exchange (ETDEWEB)

    Schols, D.; Baba, M.; Pauwels, R.; Desmyter, J.; De Clercq, E. (Katholieke Universiteit Leuven (Belgium))


    The triphenylmethane derivative aurintricarboxylic acid (ATA), but not aurin, selectively prevented the binding of OKT4A/Leu-3a monoclonal antibody (mAb) and, to a lesser extent, OKT4 mAb to the CD4 cell receptor for human immunodeficiency virus type 1 (HIV-1). The effect was seen within 1 min at an ATA concentration of 10 {mu}M in various T4{sup +} cells (MT-4, U-937, peripheral blood lymphocytes, and monocytes). It was dose-dependent and reversible. ATA prevented the attachment of radiolabeled HIV-1 particles to MT-4 cells, which could be expected as the result of its specific binding to the HIV/CD4 receptor. Other HIV inhibitors such as suramin, fuchsin acid, azidothymidine, dextran sulfate, heparin, and pentosan polysulfate did not affect OKT4A/Leu-3a mAb binding to the CD4 receptor, although the sulfated polysaccharides suppressed HIV-1 adsorption to the cells at concentrations required for complete protection against HIV-1 cytopathogenicity. Thus, ATA is a selective marker molecule for the CD4 receptor. ATA also interfered with the staining of membrane-associated HIV-1 glycoprotein gp120 by a mAb against it. These unusual properties of a small molecule of nonimmunological origin may have important implications for the study of CD4/HIV/AIDS pathogenesis and possibly treatment.

  6. Ultrafast excited state dynamics of S2 and S1 states of triphenylmethane dyes. (United States)

    Singhal, Pallavi; Ghosh, Hirendra N


    Excited state dynamics of S2 and S1 states for a series of TPM dyes, pyrogallol red (PGR), bromopyrogallol red (Br-PGR) and aurin tricarboxylic acid (ATC), have been monitored by using ultrafast transient absorption and fluorescence up-conversion techniques. Optical absorption studies indicate that all the TPM dyes exist as keto-enol tautomers depending upon the pH of the solution. Interestingly, all the TPM dyes give S2 emission (major emitting state) in addition to weak S1 emission. S2 emission lifetimes as fast as ∼150-300 fs and S1 emission lifetimes of 2-5 ns were observed depending upon the molecular structure of the dyes. Femtosecond transient absorption studies suggest the presence of an ultrafast non-radiative decay channel from the S2 state in addition to S2 luminescence. The vibrational relaxation time from hot S1 state is found to be 2-6 ps. The heavy atom effect has been observed in ultrafast relaxation dynamics of Br-PGR.

  7. Surface-secreted von Willebrand factor mediates aggregation of ADP-activated platelets at moderate shear stress: facilitated by GPIb but controlled by GPIIb-IIIa. (United States)

    Frojmovic, M M; Kasirer-Friede, A; Goldsmith, H L; Brown, E A


    We previously showed that ADP activation of washed human platelets in plasma-free suspensions supports aggregation at moderate shear stress (0.4-1.6 Nm-2) in Poiseuille flow. Although most activated platelets expressed maximal fibrinogen-occupied GPIIb-IIIa receptors, aggregation appeared to be independent of bound fibrinogen, but blocked by the hexapeptide GRGDSP. Here, we tested the hypothesis that von Willebrand factor (vWF) secreted and expressed on activated platelets mediates aggregation at moderate shear rates from 300 to 1000 s-1 corresponding to shear stresses from 0.3 to 1.1 Nm-2. Relatively unactivated platelets (Flow cytometric measurements with monoclonal antibody (mAb) 2.2.9 reporting on surface-bound vWF, and with mAb S12 reporting on alpha-granule secreted P-selectin, showed that 65% and 80%, respectively, of all platelets were maximally activated with respect to maximal secretion and surface expression of these proteins. "Resting" washed platelets exhibited both surface-bound vWF and significant P-selectin secretion. We showed that mAbs 6D1 and NMC4, respectively blocking the adhesive domains on the GPIb receptor recognizing vWF, and on the vWF molecule recognizing the GPIb receptor, partially inhibited ADP-induced aggregation under shear in Couette flow, the degree of inhibition increasing with increasing shear stress. In contrast, mAb 10E5, blocking the vWF binding domain on GPIIb-IIIa, essentially blocked all aggregation at the shear rates tested. We conclude that vWF, expressed on ADP-activated platelets, is at least the predominant cross-bridging molecule mediating aggregation at moderate shear stress. There is an absolute requirement for free activated GPIIb-IIIa receptors, postulated to interact with platelet-secreted, surface bound vWF. The GPIb-vWF cross-bridging reaction plays a facilitative role becoming increasingly important with increasing shear stress. Since aurin tricarboxylic acid, which blocks the GPIb binding domain on vWF, was

  8. The role of chronic prostatitis in the pathogenesis and progression of benign prostatic hyperplasia%慢性前列腺炎在前列腺增生症发病与进展中的作用探讨

    Institute of Scientific and Technical Information of China (English)

    张益明; 范武林; 林国太; 林永平; 陈如


    目的 探讨慢性前列腺炎在前列腺增生症发病与进展中的可能作用.方法 回顾性分析本院2011年5月至2014年12月期间因前列腺增生就诊患者356例,根据术后病理结果分为前列腺增生并慢性前列腺炎组及单纯性前列腺增生组,统计分析两组临床特征,包括:年龄(Age)、前列腺体积(PV)、PSA、IPSS评分、是否合并急性尿潴留(AUR).结果 前列腺增生并慢性前列腺炎121/356例(34.0%),发生急性尿潴留48/121例(39.7%);单纯前列腺增生235/356例(66.0%),发生尿潴留60/235例(25.5%).两组对比年龄差别无统计学意义(P>0.05);但前列腺增生合并慢性前列腺炎组较单纯前列腺增生组PV、PSA、IPSS评分、尿潴留发生率均高,差别具有统计学意义(P<0.05).结论 前列腺增生合并慢性前列腺炎通常具有更大的体积、更高的PSA及IPSS评分、更易发生尿潴留.因此,前列腺慢性炎症在前列腺增生的发病、进展中可能起作用.%Objectives To investigate the role of chronic prostatic inflammation in the pathogenesis and progression of benign prostatic hyperplasia (BPH).Methods We enrolled 356 patients with BPH from May 2011 to December 2014 in our hospital were analyzed retrospectively.According to the pathological results,all patients were divided into the BPH with prostatitis group and BPH group.Age,prostate volume (PV),PSA,IPSS,and acute urinary retention (AUR) of the two groups were analyzed.Results In the BPH with prostatitis group,121/356 (34.0%) patients were be confirmed as BPH with prostatitis,the other 48/121 (39.7%) patients were be confirmed as acute urinary retention (AUR).In the BPH group,235/356 (66.0%) patients were be comfirmed as simple BPH.and there were 60 (25.5%) patients with AUR.There was no significant difference in patient's age between the two groups (P > 0.05),but PV,PSA,IPSS and incidence of AUR in the BPH with prostatitis group were higher than BPH group (P

  9. Building Cyberinfrastructures for Earth and Space Sciences so that they will come: lessons learnt from Australia (United States)

    Wyborn, L. A.; Woodcock, R.


    environments and workflows. The eResearch Infrastructure Stack is designed to support 12 individual domain-specific capabilities. Four are relevant to the Earth and Space Sciences: (1) AuScope (a national Earth Science Infrastructure Program), (2) the Integrated Marine Observing System (IMOS), (3) the Terrestrial Ecosystems Research Network (TERN) and (4) the Australian Urban Research Infrastructure Network (AURIN). The two main research integration infrastructures, ANDS and NeCTAR, are seen as pivotal to the success of the Australian eResearch Infrastructure. Without them, there was a risk that that the investments in new computers and data storage would provide physical infrastructure, but few would come to use it as the skills barriers to entry were too high. ANDS focused on transforming Australia's research data environment. Its flagship is Research Data Australia, an Internet-based discovery service designed to provide rich connections between data, projects, researchers and institutions, and promote visibility of Australian research data collections in search engines. NeCTAR focused on building eResearch infrastructure in four areas: virtual laboratories, tools, a federated research cloud and a hosting service. Combined, ANDS and NeCTAR are ensuring that people ARE coming and ARE using the physical infrastructures that were built.