A simple and sensitive method for rapid semiquantitative and quantitative determinations of urinary protein was devised, in which protein absorbed and fixed on filter paper containing sulfosalicylic acid was reacted with Coomassie Brilliant Blue G-250 (CBB). For the semiquantitative method, filter paper (NO. 50, Toyo) was immersed in a 20% sulfosalicylic acid solution and dried. Dried filter paper was immersed in aurine, dried again immersed TONEIN (Othuka Assay Laboratories) and compared wit...
Meor Mohd Affandi MMR; Tripathy M; Shah SAA; Majeed ABA
MMR Meor Mohd Affandi,1,2 Minaketan Tripathy,1,3 Syed Adnan Ali Shah,3,4 ABA Majeed1,3 1Laboratory of Fundamental Pharmaceutics, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Bandar Puncak Alam, Selangor, Malaysia; 2DDH Core, Universiti Teknologi MARA (UiTM), Shah Alam, Selangor Darul Ehsan, Malaysia; 3Pharmaceutical and Life Sciences Core, Universiti Teknologi MARA (UiTM), Shah Alam, Selangor Darul Ehsan, Malaysia; 4Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), ...
Debnath, Tushar; Maity, Partha; Dana, Jayanta; Ghosh, Hirendra N
Wide-band-gap ZnS nanocrystals (NCs) were synthesized, and after sensitizing the NCs with series of triphenyl methane (TPM) dyes, ultrafast charge-transfer dynamics was demonstrated. HRTEM images of ZnS NCs show the formation of aggregate crystals with a flower-like structure. Exciton absorption and lumimescence, due to quantum confinement of the ZnS NCs, appear at approximately 310 and 340 nm, respectively. Interestingly, all the TPM dyes (pyrogallol red, bromopyrogallol red, and aurin tricarboxylic acid) form charge-transfer complexes with the ZnS NCs, with the appearance of a red-shifted band. Electron injection from the photoexcited TPM dyes into the conduction band of the ZnS NCs is shown to be a thermodynamically viable process, as confirmed by steady-state and time-resolved emission studies. To unravel charge-transfer (both electron injection and charge recombination) dynamics and the effect of molecular coupling, femtosecond transient absorption studies were carried out in TPM-sensitized ZnS NCs. The electron-injection dynamics is pulse-width-limited in all the ZnS/TPM dye systems, however, the back electron transfer differs, depending on the molecular coupling of the sensitizers (TPM dyes). The detailed mechanisms for the above-mentioned processes are discussed. PMID:26548569
The triphenylmethane derivative aurintricarboxylic acid (ATA), but not aurin, selectively prevented the binding of OKT4A/Leu-3a monoclonal antibody (mAb) and, to a lesser extent, OKT4 mAb to the CD4 cell receptor for human immunodeficiency virus type 1 (HIV-1). The effect was seen within 1 min at an ATA concentration of 10 μM in various T4+ cells (MT-4, U-937, peripheral blood lymphocytes, and monocytes). It was dose-dependent and reversible. ATA prevented the attachment of radiolabeled HIV-1 particles to MT-4 cells, which could be expected as the result of its specific binding to the HIV/CD4 receptor. Other HIV inhibitors such as suramin, fuchsin acid, azidothymidine, dextran sulfate, heparin, and pentosan polysulfate did not affect OKT4A/Leu-3a mAb binding to the CD4 receptor, although the sulfated polysaccharides suppressed HIV-1 adsorption to the cells at concentrations required for complete protection against HIV-1 cytopathogenicity. Thus, ATA is a selective marker molecule for the CD4 receptor. ATA also interfered with the staining of membrane-associated HIV-1 glycoprotein gp120 by a mAb against it. These unusual properties of a small molecule of nonimmunological origin may have important implications for the study of CD4/HIV/AIDS pathogenesis and possibly treatment
Meor Mohd Affandi MMR
Full Text Available MMR Meor Mohd Affandi,1,2 Minaketan Tripathy,1,3 Syed Adnan Ali Shah,3,4 ABA Majeed1,3 1Laboratory of Fundamental Pharmaceutics, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM, Bandar Puncak Alam, Selangor, Malaysia; 2DDH Core, Universiti Teknologi MARA (UiTM, Shah Alam, Selangor Darul Ehsan, Malaysia; 3Pharmaceutical and Life Sciences Core, Universiti Teknologi MARA (UiTM, Shah Alam, Selangor Darul Ehsan, Malaysia; 4Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM, Bandar Puncak Alam, Selangor, Malaysia Abstract: We examined the solubility of simvastatin in water in 0.01 mol·dm-3, 0.02 mol·dm-3, 0.04 mol·dm-3, 0.09 mol·dm-3, 0.18 mol·dm-3, 0.36 mol·dm-3, and 0.73 mol·dm-3 arginine (ARG solutions. The investigated drug is termed the solute, whereas ARG the cosolute. Phase solubility studies illustrated a higher extent of solubility enhancement for simvastatin. The aforementioned system was subjected to conductometric and volumetric measurements at temperatures (T of 298.15 K, 303.15 K, 308.15 K, and 313.15 K to illustrate the thermodynamics involved and related solute–solvent interactions. The conductance values were used to evaluate the limiting molar conductance and association constants. Thermodynamic parameters (ΔG0, ΔH0, ΔS0, and Es for the association process of the solute in the aqueous solutions of ARG were calculated. Limiting partial molar volumes and expansibilities were evaluated from the density values. These values are discussed in terms of the solute–solvent and solute–cosolute interactions. Further, these systems were analyzed using ultraviolet–visible analysis, Fourier-transform infrared spectroscopy, and 13C, 1H, and two-dimensional nuclear overhauser effect spectroscopy nuclear magnetic resonance to complement thermophysical explanation. Keywords: simvastatin–arginine complex, solubility, volumetric, conductometric
Wyborn, L. A.; Woodcock, R.
environments and workflows. The eResearch Infrastructure Stack is designed to support 12 individual domain-specific capabilities. Four are relevant to the Earth and Space Sciences: (1) AuScope (a national Earth Science Infrastructure Program), (2) the Integrated Marine Observing System (IMOS), (3) the Terrestrial Ecosystems Research Network (TERN) and (4) the Australian Urban Research Infrastructure Network (AURIN). The two main research integration infrastructures, ANDS and NeCTAR, are seen as pivotal to the success of the Australian eResearch Infrastructure. Without them, there was a risk that that the investments in new computers and data storage would provide physical infrastructure, but few would come to use it as the skills barriers to entry were too high. ANDS focused on transforming Australia's research data environment. Its flagship is Research Data Australia, an Internet-based discovery service designed to provide rich connections between data, projects, researchers and institutions, and promote visibility of Australian research data collections in search engines. NeCTAR focused on building eResearch infrastructure in four areas: virtual laboratories, tools, a federated research cloud and a hosting service. Combined, ANDS and NeCTAR are ensuring that people ARE coming and ARE using the physical infrastructures that were built.