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Sample records for aureus gatcab guarantee

  1. The essential mycobacterial amidotransferase GatCAB is a modulator of specific translational fidelity.

    Science.gov (United States)

    Su, Hong-Wei; Zhu, Jun-Hao; Li, Hao; Cai, Rong-Jun; Ealand, Christopher; Wang, Xun; Chen, Yu-Xiang; Kayani, Masood Ur Rehman; Zhu, Ting F; Moradigaravand, Danesh; Huang, Hairong; Kana, Bavesh D; Javid, Babak

    2016-01-01

    Although regulation of translation fidelity is an essential process(1-7), diverse organisms and organelles have differing requirements of translational accuracy(8-15), and errors in gene translation serve an adaptive function under certain conditions(16-20). Therefore, optimal levels of fidelity may vary according to context. Most bacteria utilize a two-step pathway for the specific synthesis of aminoacylated glutamine and/or asparagine tRNAs, involving the glutamine amidotransferase GatCAB(21-25), but it had not been appreciated that GatCAB may play a role in modulating mistranslation rates. Here, by using a forward genetic screen, we show that the mycobacterial GatCAB enzyme complex mediates the translational fidelity of glutamine and asparagine codons. We identify mutations in gatA that cause partial loss of function in the holoenzyme, with a consequent increase in rates of mistranslation. By monitoring single-cell transcription dynamics, we demonstrate that reduced gatCAB expression leads to increased mistranslation rates, which result in enhanced rifampicin-specific phenotypic resistance. Consistent with this, strains with mutations in gatA from clinical isolates of Mycobacterium tuberculosis show increased mistranslation, with associated antibiotic tolerance, suggesting a role for mistranslation as an adaptive strategy in tuberculosis. Together, our findings demonstrate a potential role for the indirect tRNA aminoacylation pathway in regulating translational fidelity and adaptive mistranslation. PMID:27564922

  2. The Guarantees of Freedom

    OpenAIRE

    Andrei Shleifer; Rafael La Porta; Florencio Lopez de Silanes; Cristian Pop-Eleches

    2002-01-01

    Hayek (1960) distinguishes the institutions of English freedom, which guarantee the independence of judges from political interference in the administration of justice, from those of American freedom, which allow judges to restrain law-making powers of the sovereign through constitutional review. We create a data base of constitutional rules in 71 countries that reflect these institutions of English and American freedom, and ask whether these rules predict economic and political freedom in a ...

  3. Refund Guarantees: On-demand bond or true guarantee?

    OpenAIRE

    Athukorale, Anjalie Narmadha

    2013-01-01

    In the modern commercial world guarantees have become an important instrument. Guarantees are not limited to those given by a bank in the commercial world. Guarantees may also be issued by a parent company on behalf of a subsidiary, or by an individual(s) in personal transactions. The question then is what is a contract of guarantee? A guarantee is an assurance or security issued by one party (the guarantor ) at the request of the party with the liability (the principal ) to the party the l...

  4. CONSIDERATIONS CONCERNING GUARANTEE SCHEMES

    Directory of Open Access Journals (Sweden)

    EMILIA CLIPICI

    2013-05-01

    Full Text Available When a large withdrawal from banks occurs, customers withdraw their deposits, so banks are likely to go bankrupt because of liquidity problems. There are several mechanisms that allow the banking system to avoid the phenomenon of massive withdrawals from banks. The most effective one is the deposit insurance. The deposit insurance is seen primarily as a means of protecting depositors of credit institutions, and secondly as a means of ensuring the stability of the banking system. This article described deposit guarantee scheme in Romania and other country.

  5. Do job security guarantees work?

    OpenAIRE

    Bryson, Alex; Cappellari, Lorenzo; Lucifora, Claudio

    2004-01-01

    We investigate the effect of employer job security guarantees on employee perceptions of job security. Using linked employer-employee data from the 1998 British Workplace Employee Relations Survey, we find job security guarantees reduce employee perceptions of job insecurity. This finding is robust to endogenous selection of job security guarantees by employers engaging in organisational change and workforce reductions. Furthermore, there is no evidence that increased job security through job...

  6. LIFECYCLE SAFETY GUARANTEE OF STRUCTURES

    Institute of Scientific and Technical Information of China (English)

    Guo Wanlin; Zhang Bin; Feng Qian; Li Chun

    2005-01-01

    The principles for lifecycle safety guarantee of engineering structures are proposed, and theconception is developed for developing the safety guarantee system by integrating the monitoringsystem, analysis system and maintenance system together on the basis of multi-sensor, distribu-tion-measurement, data fusion and digital-signal-processor (DSP) technologies as well asthree-dimensional (3D) fatigue fracture unified theory. As all the systems should work in situs and inreal-time, micromation and integration are important. Damage detectability is introduced to clarify therelationship of life prediction and healthy monitoring or faulty diagnosis. The research work to realizethe lifecycle safety guarantee system is summarized and perspectives for future efforts are outlined.

  7. Staphylococcus aureus and Pregnancy

    Science.gov (United States)

    Staphylococcus aureus and Pregnancy In every pregnancy, a woman starts out with a 3-5% chance of ... risk. This sheet talks about whether exposure to staphylococcus aureus may increase the risk for birth defects ...

  8. Infrastructure Franchising and Government Guarantees Infrastructure Franchising and Government Guarantees

    Directory of Open Access Journals (Sweden)

    Alexander Galetovic

    1998-03-01

    Full Text Available Government guarantees for private infrastructure projects reduce the incentives of firms to perform efficiently, weaken the incentives to screen projects for white elephants, and shift government obligations to future periods. Thus the use of guarantees needs to be limited, and they need to be carefully designed. Franchising schemes should in principle assign risks to the parties best able to manage and control them. The mechanisms by which contracts are awarded should be simple, so that possibilities for evaluator subjectivity are reduced, the award process remains as transparent as possible, and the likelihood of having to renegotiate is minimized. Infrastructure franchises have usually been awarded on a fixed-term basis. Such contracts expose franchise holders to considerable demand risk, which investors are often unwilling to assume without government guarantees. These contracts are also inflexible, since it is difficult to determine a fair level of compensation to the franchise holder if the contract is terminated early or modified. Under an alternative mechanism, the franchise is awarded to the firm that asks for the least present value of user fee revenue for a given tariff structure, and the franchise ends when the present value of user fee revenues is equal to the franchise holder's bid. Such contracts reduce the demand risk borne by the franchise holder (and the concomitant demand for government guarantees. They also make fair compensation of franchise holders in the event of early termination straightforward, since the level of fair compensation is equal to the revenue remaining to be collected. Government guarantees for private infrastructure projects reduce the incentives of firms to perform efficiently, weaken the incentives to screen projects for white elephants, and shift government obligations to future periods. Thus the use of guarantees needs to be limited, and they need to be carefully designed. Franchising schemes should in

  9. Staphylococcus aureus toxins.

    Science.gov (United States)

    Otto, Michael

    2014-02-01

    Staphylococcus aureus is a dangerous pathogen that causes a variety of severe diseases. The virulence of S. aureus is defined by a large repertoire of virulence factors, among which secreted toxins play a preeminent role. Many S. aureus toxins damage biological membranes, leading to cell death. In particular, S. aureus produces potent hemolysins and leukotoxins. Among the latter, some were recently identified to lyse neutrophils after ingestion, representing an especially powerful weapon against bacterial elimination by innate host defense. Furthermore, S. aureus secretes many factors that inhibit the complement cascade or prevent recognition by host defenses. Several further toxins add to this multi-faceted program of S. aureus to evade elimination in the host. This review will give an overview over S. aureus toxins focusing on recent advances in our understanding of how leukotoxins work in receptor-mediated or receptor-independent fashions.

  10. Waiting time guarantee - an update

    DEFF Research Database (Denmark)

    Socha, Karolina; Bech, Mickael

    2008-01-01

    The rule of extended free choice of hospital, in force since 2002, provides patients with an option to choose a private hospital if the public system is unable to provide a treatment within the guaranteed waiting time of one month. In June 2008 the Government declared in their yearly budget agree...... agreement with the Danish Regions a suspension of the extended free choice until 1 July 2009. This was a consequence of a force majeure situation that arose after a two-month strike of medical personnel. Udgivelsesdato: October 2008...

  11. SLA guarantees for cloud services

    OpenAIRE

    Serrano, Damián; Bouchenak, Sara; Kouki, Yousri; Alvares De Oliveira Jr., Frederico; Ledoux, Thomas; Lejeune, Jonathan; Sopena, Julien; Arantes, Luciana; Sens, Pierre

    2016-01-01

    Quality-of-service and SLA guarantees are among the major challenges of cloud-based services. In this paper we first present a new cloud model called SLAaaS — SLA aware Service. SLAaaS considers QoS levels and SLA as first class citizens of cloud-based services. This model is orthogonal to other SaaS, PaaS, and IaaS cloud models, and may apply to any of them. More specifically we make three contributions: (i) we provide a novel domain specific language that allows to describe QoS-oriented SLA...

  12. Price Guarantees, Consumer Search, and Hassle Costs

    OpenAIRE

    Baake, Pio; Schwalbe, Ulrich

    2013-01-01

    The paper deals with the competitive effects of price guarantees in a spatial duopoly where consumers can search for lower prices but have to incur hassle costs if they want to claim a price guarantee. It is shown that symmetric equilibria with and without price guarantees exist but price guarantees will have no effect on prices if search costs are low, hassle costs are high and the number of uninformed consumers is small. However, when both firms use price guarantees, there also exist payoff...

  13. 7 CFR 1720.14 - Nature of guarantee; acceleration of guaranteed bonds.

    Science.gov (United States)

    2010-01-01

    ... the terms and conditions of the bond documents; (3) The guarantee fee required by 7 CFR 1720.10 of... requirements listed in 7 CFR 1720.12; or (5) The guaranteed lender failed to comply with any material covenant... 7 Agriculture 11 2010-01-01 2010-01-01 false Nature of guarantee; acceleration of guaranteed...

  14. Government guarantees and public debt in Croatia

    Directory of Open Access Journals (Sweden)

    Anto Bajo

    2011-09-01

    Full Text Available Government fi nancial and performance guarantees have been issued in Croatia since 1996, to support funding and ensure favourable borrowing conditions in the fi nancial market for companies in majority state ownership. However, government guarantees have rarely been part of defi ned strategies and goals of public debt and risk management. Despite their steady growth, the structure of active guarantees and their infl uence on Croatian public debt are still unknown. This paper analyses the amount and structure of state guarantees, their maturities and the authority and accountability for their management, and it compares the structure of guarantees in terms of economic sectors. The main objective of the paper is to determine the infl uence of government guarantees on the public debt growth.

  15. Does Intelligence Always Guarantee Performance?

    Directory of Open Access Journals (Sweden)

    Uzma Hanif Gondal

    2013-04-01

    Full Text Available The current research is intended to explore the individuals’ cognitive intelligence and its impact on their performance. The study examines the intellectual capabilities of employees in reference to the problems that arises due to the interaction with varying job related tasks. Raven’s progressive matrices were used to measure the IQ level of employees. This IQ test was intended to measure the non-verbal intelligence, spatial intelligence, mathematical and logical intelligence of employees. It was hypothesized that individuals’ intelligence does not always guarantee performance. The final data was collected on 300 employees from different organizations of Telecom sector in the city of Lahore, Pakistan. Outcome of the analysis revealed interesting findings that Intelligence Quotient is found to be insignificantly related with individuals’ performance. It shows that IQ alone is not a strong predictor of employees’ performance. Intelligence may ensure a reach to entry level position in an organization but for a sustained position other competencies are of utmost importance. This study may provoke new lines of research in the field of positive psychology specifically.

  16. 29 CFR 4022.3 - Guaranteed benefits.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Guaranteed benefits. 4022.3 Section 4022.3 Labor Regulations Relating to Labor (Continued) PENSION BENEFIT GUARANTY CORPORATION COVERAGE AND BENEFITS BENEFITS PAYABLE IN TERMINATED SINGLE-EMPLOYER PLANS General Provisions; Guaranteed Benefits § 4022.3...

  17. Marketing service guarantees for health care.

    Science.gov (United States)

    Levy, J S

    1999-01-01

    The author introduces the concept of service guarantees for application in health care and differentiates between explicit, implicit, and conditional vs. unconditional types of guarantees. An example of an unconditional guarantee of satisfaction is provided by the hospitality industry. Firms conveying an implicit guarantee are those with outstanding reputations for products such as luxury automobiles, or ultimate customer service, like Nordstrom. Federal Express and Domino's Pizza offer explicit guarantees of on-time delivery. Taking this concept into efforts to improve health care delivery involves a number of caveats. Customers invited to use exceptional service cards may use these to record either satisfaction or dissatisfaction. The cards need to provide enough specific information about issues so that "immediate action could be taken to improve processes." Front-line employees should be empowered to respond to complaints in a meaningful way to resolve the problem before the client leaves the premises.

  18. Marketing service guarantees for health care.

    Science.gov (United States)

    Levy, J S

    1999-01-01

    The author introduces the concept of service guarantees for application in health care and differentiates between explicit, implicit, and conditional vs. unconditional types of guarantees. An example of an unconditional guarantee of satisfaction is provided by the hospitality industry. Firms conveying an implicit guarantee are those with outstanding reputations for products such as luxury automobiles, or ultimate customer service, like Nordstrom. Federal Express and Domino's Pizza offer explicit guarantees of on-time delivery. Taking this concept into efforts to improve health care delivery involves a number of caveats. Customers invited to use exceptional service cards may use these to record either satisfaction or dissatisfaction. The cards need to provide enough specific information about issues so that "immediate action could be taken to improve processes." Front-line employees should be empowered to respond to complaints in a meaningful way to resolve the problem before the client leaves the premises. PMID:10711165

  19. Guarantees in Banking Operations with Bank Customers

    Directory of Open Access Journals (Sweden)

    Vasile Dedu

    2009-07-01

    Full Text Available In a crediting relationship, one of the parties involved – namely the creditor – is exposed to certain risks which impose taking measures to guarantee a debt. If, at the due date, the debtor cannot pay his/her debts and meet the assumed liabilities, the creditor can execute the legally constituted guarantees, on a contract basis, thus covering the debt.There are also situations when the state, being directly interested in promoting trade and cooperation with other economies or simply out of the wish to ensure a competitive and safer business environment, gets involved in insurance and guarantee operations. In such situations, the state does not interve

  20. Understanding consumer response to service guarantees

    International Nuclear Information System (INIS)

    Whether the goal is customer loyalty to a competitive energy service provider or customer satisfaction with monopoly distribution services, service guarantees must be seen as one way a company can communicate with its customers about the relationship it has (or wants) with them. In short, service guarantees must be evaluated as part of a company's overall brand management strategy. To do this, a conceptual framework within which to develop and link models of service delivery and brand management is needed. This means seeing both as examples of inference management. In this article the authors first interpret findings from their research on utility service guarantees in light of the model of service delivery proposed by Smith, Bolton, and Wagner. The authors then situate this model within their own model of utility brand management. The resulting approach can be used by a utility to evaluate whether and how to adopt service guarantees as part of its overall branding strategy

  1. Staphylococcus aureus Transcriptome Architecture

    DEFF Research Database (Denmark)

    Mäder, Ulrike; Nicolas, Pierre; Depke, Maren;

    2016-01-01

    Staphylococcus aureus is a major pathogen that colonizes about 20% of the human population. Intriguingly, this Gram-positive bacterium can survive and thrive under a wide range of different conditions, both inside and outside the human body. Here, we investigated the transcriptional adaptation of S....... aureus HG001, a derivative of strain NCTC 8325, across experimental conditions ranging from optimal growth in vitro to intracellular growth in host cells. These data establish an extensive repertoire of transcription units and non-coding RNAs, a classification of 1412 promoters according...... to their dependence on the RNA polymerase sigma factors SigA or SigB, and allow identification of new potential targets for several known transcription factors. In particular, this study revealed a relatively low abundance of antisense RNAs in S. aureus, where they overlap only 6% of the coding genes, and only 19...

  2. Security Guarantees and Nuclear Non-Proliferation

    International Nuclear Information System (INIS)

    The purpose of this paper is to discuss the value of 'security guarantees', that is, positive security assurances that include a formal or informal defense commitment, in preventing nuclear proliferation. It demonstrates that such guarantees have proven to be a very effective instrument in preventing States from going nuclear. It would thus seem logical to reinforce or extend them. However, this path is fraught with obstacles and dilemmas

  3. Guaranteed cost control for networked control systems

    Institute of Scientific and Technical Information of China (English)

    Linbo XIE; Huajing FANG; Ying ZHENG

    2004-01-01

    The guaranteed cost control problem for networked control systems (NCSs) is addressed under communication constraints and varying sampling rate. First of all, a simple information-scheduling scheme is presented to describe the scheduling approach of system signals in NCSs. Then, based on such a scheme and given sampling method, the design procedure in dynamic output feedback manner is also derived which renders the closed loop system to be asymptotically stable and guarantees an upper bound of the LQ performance cost function.

  4. CREDIT SYSTEM AND CREDIT GUARANTEE PROGRAMS

    OpenAIRE

    Turgay GECER

    2012-01-01

    Credit system is an integrated architecture consisted of financial information, credit rating, credit risk management, receivables and credit insurance systems, credit derivative markets and credit guarantee programs. The main purpose of the credit system is to provide the functioning of all credit channels and to make it easy to access of credit sources demanded by all of real and legal persons in any economic system. Credit guarantee program, the one of prominent elements of the credit syst...

  5. Insights into tRNA-Dependent Amidotransferase Evolution and Catalysis from the Structure of the Aquifex aeolicus Enzyme

    OpenAIRE

    WU, Jing; Bu, Weishu; Sheppard, Kelly; Kitabatake, Makoto; Kwon, Suk-Tae; Söll, Dieter; Smith, Janet L.

    2009-01-01

    Many bacteria form Gln-tRNAGln and Asn-tRNAAsn by conversion of the misacylated Glu-tRNAGln and Asp-tRNAAsn species catalyzed by the GatCAB amidotransferase in the presence of ATP and an amide donor (glutamine or asparagine). Here we report the crystal structures of GatCAB from the hyperthermophilic bacterium Aquifex aeolicus complexed with glutamine, asparagine, aspartate, ADP, or ATP. In contrast to the Staphylococcus aureus GatCAB, the A. aeolicus enzyme formed acyl-enzyme intermediates wi...

  6. MODELLING THE GUARANTEE LIABILITY UNDER UNIT-LINKED CONTRACTS

    OpenAIRE

    Cristina CIUMAȘ; Diana-Maria CHIȘ

    2015-01-01

    Unit-linked contracts are wrapped with some death, maturity and accumulation guarantees such as the guaranteed minimum maturity benefit, the guaranteed minimum death benefit, the guaranteed minimum accumulation benefit, the guaranteed minimum income benefit, and/or the guaranteed minimum surrender benefit. According to Romanian legislation which regulates the unit-linked life insurance market, unit-linked life insurance contracts pass most of the investment risk to the policyholder and involv...

  7. 76 FR 1 - Continuous Construction-Permanent Loan Guarantees Under the Section 538 Guaranteed Rural Rental...

    Science.gov (United States)

    2011-01-03

    ... CFR Part 3565 RIN 0575-AC80 Continuous Construction-Permanent Loan Guarantees Under the Section 538... Rural Housing Service (RHS) (an agency within the Rural Development mission area) is amending its... Housing Program. A single, continuous guarantee during the construction phase for construction...

  8. 7 CFR 762.160 - Assignment of guarantee.

    Science.gov (United States)

    2010-01-01

    ... of the loan. The holder must assign the guaranteed portion back to the original lender if requested... AGRICULTURE SPECIAL PROGRAMS GUARANTEED FARM LOANS § 762.160 Assignment of guarantee. (a) The following general requirements apply to assigning guaranteed loans: (1) Subject to Agency concurrence, the...

  9. Large-Scale Similarity Joins With Guarantees

    DEFF Research Database (Denmark)

    Pagh, Rasmus

    2015-01-01

    The ability to handle noisy or imprecise data is becoming increasingly important in computing. In the database community the notion of similarity join has been studied extensively, yet existing solutions have offered weak performance guarantees. Either they are based on deterministic filtering te...

  10. 46 CFR 298.36 - Guarantee Fee.

    Science.gov (United States)

    2010-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VESSEL FINANCING ASSISTANCE OBLIGATION GUARANTEES Documentation... generally shall vary inversely with the ratio of Equity to Long-Term Debt (Variable Rate) of the Person who... computation of Equity and Long-Term Debt considered necessary to reflect more accurately the...

  11. Learning Good Edit Similarities with Generalization Guarantees

    OpenAIRE

    Bellet, Aurélien; Habrard, Amaury; Sebban, Marc

    2011-01-01

    Similarity and distance functions are essential to many learning algorithms, thus training them has attracted a lot of interest. When it comes to dealing with structured data (e.g., strings or trees), edit similarities are widely used, and there exists a few methods for learning them. However, these methods offer no theoretical guarantee as to the generalization performance and discriminative power of the resulting similarities. Recently, a theory of learning with good similarity functions wa...

  12. China and the Multilateral Investment Guarantee Agency

    OpenAIRE

    Yushu Feng

    1997-01-01

    The Multilateral Investment Guarantee Agency (MIGA), established in 1988, is part of the World Bank Group. Its mandate is to promote foreign investment in its member countries by providing political risk insurance and technical assistance to promote investment. In developing this case study of China's evolving relationship with MIGA, the author asked: Why did China decide to seek membership in MIGA? What was the outcome of the relationship between China and MIGA? How has that relationship aff...

  13. Enigma: Decentralized Computation Platform with Guaranteed Privacy

    OpenAIRE

    Zyskind, Guy; Nathan, Oz; Pentland, Alex

    2015-01-01

    A peer-to-peer network, enabling different parties to jointly store and run computations on data while keeping the data completely private. Enigma's computational model is based on a highly optimized version of secure multi-party computation, guaranteed by a verifiable secret-sharing scheme. For storage, we use a modified distributed hashtable for holding secret-shared data. An external blockchain is utilized as the controller of the network, manages access control, identities and serves as a...

  14. 75 FR 63419 - Surety Bond Guarantee Program; Timber Sales

    Science.gov (United States)

    2010-10-15

    ...The Small Business Administration (SBA) proposes to amend its Surety Bond Guarantee Program rules to guarantee performance bonds for timber sale contracts awarded by the Federal Government or other public or private...

  15. Linezolid resistant Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Pavani Gandham

    2014-08-01

    Full Text Available Linezolid is the only antibiotic available as an oral formulation for resistant staphylococcal infections. It is effective in skin and soft tissue infections, nosocomial pneumonias including VAP, infective endocarditis and MRSA meningitis. It is also effective in the eradication of both nasal and throat colonization of MRSA. Its high bioavailability and post antibiotic effect, ease of switching to oral therapy during its use and the fact that it can be used in patients of all ages, also in patients with liver disease and poor kidney function and its increased effectiveness over glycopeptides makes this drug a precious drug in the treatment of resistant staphylococcal infections. Linezolid resistance in staphylococcus is defined as a linezolid MIC of and #8805;8 mg/L. Reported Linezolid resistance in India and elsewhere is 2-20%. There is clonal dissemination of Linezolid Resistant Staphylococcus aureus (LRSA within or across health care settings which demands continuous surveillance to determine the emergent risk of resistance strains and to establish guidelines for appropriate use. Clinical laboratories should confirm any LRSA preferably by a second method, prior to using linezolid for serious infections. Effective surveillance, more judicious use of this antibiotic, avoiding linezolid usage for empiric therapy in hospital acquired staphylococcus infections, optimization of the pharmacological parameters of the antibiotics in specific clinical situation, decreasing bacterial load by timely surgical debridement or drainage of collections, use of combination therapies would prevent the emergence of resistance to linezolid in staphylococcus aureus. [Int J Res Med Sci 2014; 2(4.000: 1253-1256

  16. 7 CFR 762.145 - Restructuring guaranteed loans.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 7 2010-01-01 2010-01-01 false Restructuring guaranteed loans. 762.145 Section 762..., DEPARTMENT OF AGRICULTURE SPECIAL PROGRAMS GUARANTEED FARM LOANS § 762.145 Restructuring guaranteed loans. (a... approval of the Agency for all restructuring actions; and, (ii) Provide the items in paragraph (b) and...

  17. 7 CFR 1717.1207 - RUS obligations under loan guarantees.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 11 2010-01-01 2010-01-01 false RUS obligations under loan guarantees. 1717.1207... ELECTRIC LOANS Settlement of Debt § 1717.1207 RUS obligations under loan guarantees. Nothing in this subpart affects the obligations of RUS under loan guarantee commitments it has made to the...

  18. 29 CFR 4022.62 - Estimated guaranteed benefit.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Estimated guaranteed benefit. 4022.62 Section 4022.62 Labor... PAYABLE IN TERMINATED SINGLE-EMPLOYER PLANS Benefit Reductions in Terminating Plans § 4022.62 Estimated guaranteed benefit. (a) General. The estimated guaranteed benefit payable with respect to each...

  19. 7 CFR 4279.149 - Personal and corporate guarantee.

    Science.gov (United States)

    2010-01-01

    ...-COOPERATIVE SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE GUARANTEED LOANMAKING Business and Industry Loans § 4279.149 Personal and corporate guarantee. (a) Unconditional personal and corporate... 7 Agriculture 15 2010-01-01 2010-01-01 false Personal and corporate guarantee. 4279.149...

  20. 10 CFR 609.10 - Loan Guarantee Agreement.

    Science.gov (United States)

    2010-01-01

    ... accepted accounting principles and practices. The non-guaranteed portion (if any) of any Guaranteed...; (7) The loan guarantee does not finance, either directly or indirectly, tax-exempt debt obligations... made during the regular business hours of the Borrower, Eligible Lender or other Holder, or other...

  1. 13 CFR 120.348 - Amount of guarantee.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Amount of guarantee. 120.348 Section 120.348 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Special Purpose Loans International Trade Loans § 120.348 Amount of guarantee. SBA can guarantee up to...

  2. Staphylococcus aureus triggered reactive arthritis.

    OpenAIRE

    Siam, A R; M. Hammoudeh

    1995-01-01

    OBJECTIVES--To report two patients who developed reactive arthritis in association with Staphylococcus aureus infection. METHODS--A review of the case notes of two patients. RESULTS--Two adult female patients have developed sterile arthritis in association with Staph aureus infection. The first patient has had two episodes of arthritis; the first followed olecranon bursitis, the second followed infection of a central venous catheter used for dialysis. The second patient developed sterile arth...

  3. Guaranteeing CERN’s excellence: consolidate experience

    CERN Multimedia

    Staff Association

    2014-01-01

    For its missions CERN requires staff with solid experience in all its domains of activity The Organization has several missions: fundamental research, technical developments and innovation, training the several hundreds of associates, fellows and students, while at the same time taking care of more than 10000 users. In order to guarantee excellence in all of these areas, CERN has to put in place an efficient personnel policy. Such a policy must allow the Organization to recruit collaborators with the highest competence from all Member States, and to keep and motivate them during their entire professional career. But, more importantly, the Organization has to be able to count on a stable workforce. It needs staff with experience gained over a long period in the fields of accelerators, detectors, and operating procedures, if it is to fulfil successfully its important mission of training and knowledge transfer, which is very much appreciated by our Member states, since it highlights a visible return on invest...

  4. Guaranteed clustering and biclustering via semidefinite programming

    CERN Document Server

    Ames, Brendan P W

    2012-01-01

    Identifying clusters of similar objects in data plays a significant role in a wide range of applications. As a model problem for clustering, we consider the densest k-disjoint-clique problem, whose goal is to identify the collection of k disjoint cliques of a given weighted complete graph maximizing the sum of the densities of the complete subgraphs induced by these cliques. In this paper, we establish conditions ensuring exact recovery of the densest k cliques of a given graph from the optimal solution of a particular semidefinite program. In particular, the semidefinite relaxation is exact for input graphs corresponding to data consisting of k large, distinct clusters and a smaller number of outliers. This approach also yields a semidefinite relaxation for the biclustering problem with similar recovery guarantees. Given a set of objects and a set of features exhibited by these objects, biclustering seeks to simultaneously group the objects and features according to their expression levels. This problem may ...

  5. Framework for analysis of guaranteed QOS systems

    Science.gov (United States)

    Chaudhry, Shailender; Choudhary, Alok

    1997-01-01

    Multimedia data is isochronous in nature and entails managing and delivering high volumes of data. Multiprocessors with their large processing power, vast memory, and fast interconnects, are an ideal candidate for the implementation of multimedia applications. Initially, multiprocessors were designed to execute scientific programs and thus their architecture was optimized to provide low message latency and efficiently support regular communication patterns. Hence, they have a regular network topology and most use wormhole routing. The design offers the benefits of a simple router, small buffer size, and network latency that is almost independent of path length. Among the various multimedia applications, video on demand (VOD) server is well-suited for implementation using parallel multiprocessors. Logical models for VOD servers are presently mapped onto multiprocessors. Our paper provides a framework for calculating bounds on utilization of system resources with which QoS parameters for each isochronous stream can be guaranteed. Effects of the architecture of multiprocessors, and efficiency of various local models and mapping on particular architectures can be investigated within our framework. Our framework is based on rigorous proofs and provides tight bounds. The results obtained may be used as the basis for admission control tests. To illustrate the versatility of our framework, we provide bounds on utilization for various logical models applied to mesh connected architectures for a video on demand server. Our results show that worm hole routing can lead to packets waiting for transmission of other packets that apparently share no common resources. This situation is analogous to head-of-the-line blocking. We find that the provision of multiple VCs per link and multiple flit buffers improves utilization (even under guaranteed QoS parameters). This analogous to parallel iterative matching.

  6. Staphylococcus aureus Bacteraemia

    Directory of Open Access Journals (Sweden)

    James Price

    2010-01-01

    Full Text Available Staphylococcus aureus bacteraemia (SAB is commonly complicated by metastatic infection or relapse after treatment. Objectives. The study aim was to determine the role of bacterial, host, and management factors in development of complicated SAB. Methods. A prospectively-conducted observational study gathered data on predisposition, management and outcome of 100 consecutive SAB cases. Antibiotic susceptibilities and genetic lineage of bacterial isolates were determined. Further clinical and microbiological data were gathered on two retrospective series from 1999–2000 (n=57 and 2004 (n=116. Results. In the prospective cases, 27% met our definition of complicated disease. Expressed as RR and 95% CI, complicated disease was associated with diabetes (1.58, 1.00–2.48, injecting-drug use (5.48, 0.88–33.49, community-onset of symptoms (1.4, 1.02–1.92, and symptom duration ≥48 hours prior to starting effective antibiotic therapy (2.10, 1.22–3.61. Uncomplicated disease was associated with the presence of a central line (0.69, 0.55–0.88 and prompt removal of a primary focus (0.71, 0.57–0.90. Neither methicillin resistance nor genetic lineage was associated with complicated disease, but methicillin resistance was associated with higher mortality. Conclusions. This study demonstrates that clinical rather than microbial factors are the major determinants of SAB outcome and underscores the importance of early treatment.

  7. Valuation and Efficient Allocation of GSM Export Credit Guarantees

    OpenAIRE

    Diersen, Matthew A.; Sherrick, Bruce J.

    2005-01-01

    Estimates of country-level loan default distributions are developed and used in a loan guarantee model to value the contingent liability of USDA's General Sales Manager (GSM) export credit guarantee portfolio. The results quantify the relationship between increasing guarantee coverage and the resulting actuarial liability to the government. Optimal coverage levels and optimal country-level allocations are determined for given policy objectives and coverage totals. Findings reveal that the gov...

  8. Switching Fuzzy Guaranteed Cost Control for Nonlinear Networked Control Systems

    Directory of Open Access Journals (Sweden)

    Linqin Cai

    2014-01-01

    Full Text Available This paper deals with the problem of guaranteed cost control for a class of nonlinear networked control systems (NCSs with time-varying delay. A guaranteed cost controller design method is proposed to achieve the desired control performance based on the switched T-S fuzzy model. The switching mechanism is introduced to handle the uncertainties of NCSs. Based on Lyapunov functional approach, some sufficient conditions for the existence of state feedback robust guaranteed cost controller are presented. Simulation results show that the proposed method is effective to guarantee system’s global asymptotic stability and quality of service (QoS.

  9. Challenges of guarantee-time bias.

    Science.gov (United States)

    Giobbie-Hurder, Anita; Gelber, Richard D; Regan, Meredith M

    2013-08-10

    The potential for guarantee-time bias (GTB), also known as immortal time bias, exists whenever an analysis that is timed from enrollment or random assignment, such as disease-free or overall survival, is compared across groups defined by a classifying event occurring sometime during follow-up. The types of events associated with GTB are varied and may include the occurrence of objective disease response, onset of toxicity, or seroconversion. However, comparative analyses using these types of events as predictors are different from analyses using baseline characteristics that are specified completely before the occurrence of any outcome event. Recognizing the potential for GTB is not always straightforward, and it can be challenging to know when GTB is influencing the results of an analysis. This article defines GTB, provides examples of GTB from several published articles, and discusses three analytic techniques that can be used to remove the bias: conditional landmark analysis, extended Cox model, and inverse probability weighting. The strengths and limitations of each technique are presented. As an example, we explore the effect of bisphosphonate use on disease-free survival (DFS) using data from the BIG (Breast International Group) 1-98 randomized clinical trial. An analysis using a naive approach showed substantial benefit for patients who received bisphosphonate therapy. In contrast, analyses using the three methods known to remove GTB showed no statistical evidence of a reduction in risk of a DFS event with bisphosphonate therapy.

  10. Mild Staphylococcus aureus skin infection improves the course of subsequent endogenous S. aureus bacteremia in mice

    NARCIS (Netherlands)

    S. van den Berg (Sanne); C.P. de Vogel (Corné); A.F. van Belkum (Alex); I.A.J.M. Bakker-Woudenberg (Irma)

    2015-01-01

    textabstractStaphylococcus aureus carriers with S. aureus bacteremia may have a reduced mortality risk compared to non-carriers. A role for the immune system is suggested. Here, we study in mice the effect of mild S. aureus skin infection prior to endogenous or exogenous S. aureus bacteremia, and ev

  11. Host-adaptive evolution of Staphylococcus aureus

    OpenAIRE

    Lowder, Bethan Victoria

    2011-01-01

    Staphylococcus aureus is a notorious human pathogen associated with severe nosocomial and community-acquired infections. In addition, S. aureus is a major cause of animal diseases including skeletal infections of poultry and bovine and ovine mastitis, which are a large economic burden on the broiler chicken and dairy farming industries. The population structure of S. aureus associated with humans has been well studied. However, despite the prevalence of S. aureus infections in ...

  12. [Protein toxins of Staphylococcus aureus].

    Science.gov (United States)

    Shamsutdinov, A F; Tiurin, Iu A

    2014-01-01

    Main scientific-research studies regarding protein bacterial toxins of the most widespread bacteria that belong to Staphylococcus spp. genus and in particular the most pathogenic species for humans--Staphylococcus aureus, are analyzed. Structural and biological properties of protein toxins that have received the name of staphylococcus pyrogenic toxins (PTSAg) are presented. Data regarding genetic regulation of secretion and synthesis of these toxins and 3 main regulatory genetic systems (agr--accessory gene regulator, xpr--extracellular protein regulator, sar--staphylococcal accessory regulator) that coordinate synthesis of the most important protein toxins and enzymes for virulence of S. aureus, are presented.

  13. Prevention of Healthcare Associated Staphylococcus aureus Infections

    NARCIS (Netherlands)

    L.G.M. Bode (Lonneke)

    2014-01-01

    markdownabstract__Abstract__ S. aureus colonizes the skin and mucosae of a proportion of the human population. Carriers of S. aureus are at increased risk of developing infections with this pathogen. The aim of this thesis was to add to the prevention of healthcare associated S. aureus infections.

  14. 7 CFR 1980.488 - Guaranteed industrial development bond issues.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 14 2010-01-01 2009-01-01 true Guaranteed industrial development bond issues. 1980... Program § 1980.488 Guaranteed industrial development bond issues. (a) Loans to public bodies will be... its successor agency under Public Law 103-354 of the taxability of the proposed bond issue....

  15. 77 FR 40785 - Single Family Housing Guaranteed Loan Program

    Science.gov (United States)

    2012-07-11

    ... affect only Agency determination of program benefits for guarantees of loans made to individuals. Changes... loans on their books for 30 years. Most small community banks originate the loans and immediately sell...-AC90 Single Family Housing Guaranteed Loan Program AGENCIES: Rural Housing Service, Rural...

  16. 48 CFR 52.247-60 - Guaranteed Shipping Characteristics.

    Science.gov (United States)

    2010-10-01

    ... Characteristics. 52.247-60 Section 52.247-60 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION....247-60 Guaranteed Shipping Characteristics. As prescribed in 47.305-16(b)(1), insert the following clause: Guaranteed Shipping Characteristics (DEC 1989) (a) The offeror is requested to...

  17. Stress Responses in Staphylococcus aureus

    DEFF Research Database (Denmark)

    Frees, Dorte; Ingmer, Hanne

    2016-01-01

    Staphylococcus aures are prominent members of the normal flora of humans and animals, but are also a major cause of mild and severe infections. To persist and disseminate in the human host, and to survive in environmental settings, such as hospitals, S. aureus have developed a plethora of cellular...

  18. Staphylococcus aureus and hand eczema severity

    DEFF Research Database (Denmark)

    Haslund, P; Bangsgaard, N; Jarløv, J O;

    2009-01-01

    BACKGROUND: The role of bacterial infections in hand eczema (HE) remains to be assessed. OBJECTIVES: To determine the prevalence of Staphylococcus aureus in patients with HE compared with controls, and to relate presence of S. aureus, subtypes and toxin production to severity of HE. METHODS......: Bacterial swabs were taken at three different visits from the hand and nose in 50 patients with HE and 50 controls. Staphylococcus aureus was subtyped by spa typing and assigned to clonal complexes (CCs), and isolates were tested for exotoxin-producing S. aureus strains. The Hand Eczema Severity Index...... was used for severity assessment. RESULTS: Staphylococcus aureus was found on the hands in 24 patients with HE and four controls (P aureus was found to be related to increased severity of the eczema (P aureus types on the hands...

  19. Empirical research on the SMEs' credit-guarantee in China

    Institute of Scientific and Technical Information of China (English)

    贾生华; 陈宏辉

    2002-01-01

    After the authors' thorough study of the experiments of other countries and regions, it is posed that credit-guarantee is the key for solving the problem of the difficulties of SMEs' financing. Based on two surveys and interviews with 57 SMEs and some commercial banks' staff, this study on the practice and implement action of SMEs' credit-guarantee revealed some problems existing in the practical process. A series of policy suggestions are given for improving the SMEs' credit-guarantee in China.

  20. Empirical research on the SMEs' credit-guarantee in China

    Institute of Scientific and Technical Information of China (English)

    贾生华; 陈宏辉

    2002-01-01

    After the authors' thorough study of the experiments of other countries and regions, it is posed that credit-guarantee is the key for solving the problem of the difficulties of SMEs' financing.Based on two surveys and interviews with 57 SMEs and some commercial banks' staff, this study on the practice and implement action of SMEs' credit-guarantee revealed some problems existing in the practical process. A series of policy suggestions are given for improving the SMEs' credit-guarantee in China.

  1. Current rules of the deposit guarantee system in Poland

    Directory of Open Access Journals (Sweden)

    Patrycja Zawadzka

    2011-06-01

    Full Text Available The article considers the matter of the deposit guarantee system in Poland, which is coordinated by the Bank Guarantee Fund (BFG. Presented in the first part are the organization of the BFG and its tasks, such as guaranteeing, aiding, controlling and analyzing the banking sector. Discussed in the publication are changes in Polish law, especially in the reaction to the global crisis on the financial market in the late 2000s. Finally, the future outlook for EU regulation is presented, along with a number of conclusions on the potential legislative changes in the Act on the BFG. The main goal of the article is to characterize details of the Polish implementation of Directives 94/19/EC and 2009/14/EC, as well as the actual form of the deposit guarantee system, and to answer the question of whether the Act on the BFG corresponds to the EU directives.

  2. Reconfiguring the Logical Topology With Performance Guarantees in WDM Networks

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    To improve the network performance after traffic demand changes, reconfiguring the logical topology is necessary. We present an ILP algorithm to find out the least lightpath changes needed with guaranteed network performance.

  3. [Waiting lists guarantee in health care. Some theoretical reflections].

    Science.gov (United States)

    Piene, H; Hauge, H K; Nyen, P A

    1997-01-30

    In 1990 a "waiting list guarantee" was introduced in the Norwegian health care system to secure treatment within six months for patients belonging to priority group II. (Priority group II are patients in need of treatment to avoid health hazards or serious long-term effects.) This guarantee has been difficult to honour and has caused considerable political unrest in the recent years. In an attempt to reform the guarantee, an analysis of our hospitals' capacity problems has been carried out, based on the general theory of queues. One result was that in order to fulfill the guarantee it is necessary to drastically reduce the queues and increase the capacity to deliver health services. This article presents the reasoning behind the analysis, in order to demonstrate the necessary foundation for a health policy that aims to reduce the time Norwegian patients have to wait for treatment in hospital. PMID:9064860

  4. EXTERNAL EVALUATION OF LEARNING AS A GUARANTEE OF QUALITY EDUCATION

    Directory of Open Access Journals (Sweden)

    José Mª Arribas Estebaranz

    2014-12-01

    Almost all countries and all reports use contemplated, in varying degrees, the always-complementary external evaluation by the teachers-as an essential guarantee of educational quality at different levels of detail curriculum evaluation.

  5. 13 CFR 120.524 - When is SBA released from liability on its guarantee?

    Science.gov (United States)

    2010-01-01

    ... liability on its guarantee? 120.524 Section 120.524 Business Credit and Assistance SMALL BUSINESS... Purchase of A Guaranteed Portion § 120.524 When is SBA released from liability on its guarantee? (a) SBA is... from its guarantee only if the Lender fails to request that SBA purchase the guarantee within 180...

  6. DEPOSIT GUARANTEE SCHEMES JOIN FINANCIAL SAFETY-NET

    OpenAIRE

    Eugen Dijmarescu

    2011-01-01

    Deposit Guarantee Schemes (DGS) become more visible under the current conditions of the world financial markets. If those have played in the past a rather discreet role for social stability through the function of pay box following a bank failure, the recent crisis put them in a new light, once the guarantee ceiling for eligible deposits grew in order to make for the accumulation of wealth worldwide and the increased risk incurred by the leverage of banking operations. Consequently, the resou...

  7. LEGAL GUARANTEES OF FARMERS’ TRADE UNIONS’ FUNCTIONING IN POLAND

    OpenAIRE

    Jakub Gołaś

    2016-01-01

    The main purpose of the article is to present legal basis and guarantees of the farmers’ trade unions’ functioning in Poland. The presented research was based on a thorough examination of national legal acts, which constitute both legal background and foundation of farmer’s unions’ activities and internal structure. The author tries to introduce both the individual legal and system guarantees which are dedicated to farmers’ organization and which exact acts shall...

  8. Immunomodulation and Disease Tolerance to Staphylococcus aureus

    OpenAIRE

    Zhigang Li; Peres, Adam G.; Andreea C. Damian; Joaquín Madrenas

    2015-01-01

    The Gram-positive bacterium Staphylococcus aureus is one of the most frequent pathogens that causes severe morbidity and mortality throughout the world. S. aureus can infect skin and soft tissues or become invasive leading to diseases such as pneumonia, endocarditis, sepsis or toxic shock syndrome. In contrast, S. aureus is also a common commensal microbe and is often part of the human nasal microbiome without causing any apparent disease. In this review, we explore the immunomodulation and d...

  9. Postoperative Staphylococcus aureus infections in Medicare beneficiaries.

    Directory of Open Access Journals (Sweden)

    Moaven Razavi

    Full Text Available Staphylococcus aureus (S. aureus infections are important because of their increasing frequency, resistance to antibiotics, and high associated rates of disabilities and deaths. We examined the incidence and correlates of S. aureus infections following 219,958 major surgical procedures in a 5% random sample of fee-for-service Medicare beneficiaries from 2004-2007. Of these surgical patients, 0.3% had S. aureus infections during the hospitalizations when index surgical procedures were performed; and 1.7% and 2.3%, respectively, were hospitalized with infections within 60 days or 180 days following admissions for index surgeries. S. aureus infections occurred within 180 days in 1.9% of patients following coronary artery bypass graft surgery, 2.3% following hip surgery, and 5.9% following gastric or esophageal surgery. Of patients first hospitalized with any major infection reported during the first 180 days after index surgery, 15% of infections were due to S. aureus, 18% to other documented organisms, and no specific organism was reported on claim forms in 67%. Patient-level predictors of S. aureus infections included transfer from skilled nursing facilities or chronic hospitals and comorbid conditions (e.g., diabetes, congestive heart failure, chronic obstructive pulmonary disease, and chronic renal disease. In a logarithmic regression, elective index admissions with S. aureus infection stayed 130% longer than comparable patients without that infection. Within 180 days of the index surgery, 23.9% of patients with S. aureus infection and 10.6% of patients without this infection had died. In a multivariate logistic regression of death within 180 days of admission for the index surgery with adjustment for demographics, co-morbidities, and other risks, S. aureus was associated with a 42% excess risk of death. Due to incomplete documentation of organisms in Medicare claims, these statistics may underestimate the magnitude of S. aureus infection

  10. Immunomodulation and Disease Tolerance to Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Zhigang Li

    2015-11-01

    Full Text Available The Gram-positive bacterium Staphylococcus aureus is one of the most frequent pathogens that causes severe morbidity and mortality throughout the world. S. aureus can infect skin and soft tissues or become invasive leading to diseases such as pneumonia, endocarditis, sepsis or toxic shock syndrome. In contrast, S. aureus is also a common commensal microbe and is often part of the human nasal microbiome without causing any apparent disease. In this review, we explore the immunomodulation and disease tolerance mechanisms that promote commensalism to S. aureus.

  11. Mastite com lesões sistêmicas por Staphylococus aureus subesp. aureus em coelhos Mastitis with systemic lesions due to Staphylococus aureus subesp. aureus in rabbits

    OpenAIRE

    Sandra Davi Traverso; Leonardo da Cunha; Joaquim César Teixeira Fernandes; Alexandre Paulino Loretti; Adriana Rhoden; Elsio Wunder Jr.; David Driemeier

    2003-01-01

    Em uma criação composta por 1800 coelhos, 33% das matrizes apresentaram mastite e lesões cutâneas crostosas e purulentas. Estes animais apresentavam-se entre 10 a- 12 meses de idade e em segunda parição. Quinze coelhos afetados foram sacrificados e necropsiados. Na necropsia, além das lesões cutâneas haviam microabscessos em diversos órgãos. Das amostras coletadas isolou-se Staphylococcus aureus subesp. aureus. S. aureus subesp. aureus também foi isolado de "swab" nasal coletado do tratador e...

  12. Methicillin-Resistant Staphylococcus aureus (MRSA) Treatment

    Science.gov (United States)

    ... Laboratory of Bacteriology Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) Antibacterial Resistance Leadership Group (ARLG) NIAID Antimicrobial Resistance Funding Information ...

  13. Methicillin-Resistant Staphylococcus aureus (MRSA) Diagnosis

    Science.gov (United States)

    ... Laboratory of Bacteriology Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) Antibacterial Resistance Leadership Group (ARLG) NIAID Antimicrobial Resistance Funding Information ...

  14. Mild Staphylococcus aureus Skin Infection Improves the Course of Subsequent Endogenous S. aureus Bacteremia in Mice.

    Directory of Open Access Journals (Sweden)

    Sanne van den Berg

    Full Text Available Staphylococcus aureus carriers with S. aureus bacteremia may have a reduced mortality risk compared to non-carriers. A role for the immune system is suggested. Here, we study in mice the effect of mild S. aureus skin infection prior to endogenous or exogenous S. aureus bacteremia, and evaluate protection in relation to anti-staphylococcal antibody levels. Skin infections once or twice by a clinical S. aureus isolate (isolate P or S. aureus strain 8325-4 were induced in mice free of S. aureus and anti-staphylococcal antibodies. Five weeks later, immunoglobulin G (IgG levels in blood against 25 S. aureus antigens were determined, and LD50 or LD100 bacteremia caused by S. aureus isolate P was induced. S. aureus skin infections led to elevated levels of anti-staphylococcal IgG in blood. One skin infection improved the course of subsequent severe endogenous bacteremia only. A second skin infection further improved animal survival rate, which was associated with increased pre-bacteremia IgG levels against Efb, IsaA, LukD, LukE, Nuc, PrsA and WTA. In conclusion, S. aureus isolate P skin infection in mice reduces the severity of subsequent endogenous S. aureus bacteremia only. Although cellular immune effects cannot be rules out, anti-staphylococcal IgG against specified antigens may contribute to this effect.

  15. Characterisation of Staphylococcus aureus bacteraemia at Tygerberg hospital

    NARCIS (Netherlands)

    Orth, H.; Dreyer, Z.S.; Makgotlho, E.; Oosthuysen, W.; Sinha, B.; Wasserman, E.

    2013-01-01

    To elucidate the local epidemiology of Staphylococcus aureus bacteraemia, we characterised blood culture isolates using molecular methods and prospectively collected clinical data to determine the occurrence of community-acquired, methicillinresistant S. aureus (MRSA). Consecutive S. aureus blood cu

  16. Evaluation of Two New Chromogenic Media, CHROMagar MRSA and S. aureus ID, for Identifying Staphylococcus aureus and Screening Methicillin-Resistant S. aureus

    OpenAIRE

    Hedin, Göran; Fang, Hong

    2005-01-01

    Thirty-nine methicillin-resistant Staphylococcus aureus (MRSA) isolates with diverse genetic backgrounds and two reference strains were correctly identified as S. aureus on CHROMagar MRSA and S. aureus ID media. Growth inhibition on CHROMagar MRSA was noted. A combination of cefoxitin disk and S. aureus ID was found suitable for rapid MRSA screening.

  17. LEGAL GUARANTEES OF FARMERS’ TRADE UNIONS’ FUNCTIONING IN POLAND

    Directory of Open Access Journals (Sweden)

    Jakub Gołaś

    2016-03-01

    Full Text Available The main purpose of the article is to present legal basis and guarantees of the farmers’ trade unions’ functioning in Poland. The presented research was based on a thorough examination of national legal acts, which constitute both legal background and foundation of farmer’s unions’ activities and internal structure. The author tries to introduce both the individual legal and system guarantees which are dedicated to farmers’ organization and which exact acts shall be considered as the direct legal basis of the guarantees. Having regard to the fact that even the Polish Constitution introduces transparent and direct legal frameworks for functioning of farmers’ trade unions, the author intends to prove that individual farmers’ unions’ operations should be considered as a fundamental and basic feature of the national agricultural system. The presented work should be also considered as thorough examination of legal provisions presented in the Act on individual farmers’ trade unions of 7th April 1989 (Ustawa…, 2015b, which is direct legal basis for functioning guarantees of the agricultural unions. The author shows how the Act is governing such fundamental issues as creation, registration, liquidation or legal operational guarantees of each individual farmers’ trade unions operating under the Act.

  18. Immunogenicity of toxins during Staphylococcus aureus infection

    NARCIS (Netherlands)

    N.J. Verkaik (Nelianne); O. Dauwalder (Olivier); K. Antri (Kenza); I. Boubekri (Ilhem); C.P. de Vogel (Corné); C. Badiou (Cédric); M. Bes (Michèle); F. Vandenesch (François); M. Tazir (Mohammed); H. Hooijkaas (Herbert); H.A. Verbrugh (Henri); A.F. van Belkum (Alex); J. Etienne (Jerome); G. Lina (Gérard); N. Ramdani-Bouguessa (Nadjia); W.J.B. van Wamel (Willem)

    2010-01-01

    textabstractAB - BACKGROUND: Toxins are important Staphylococcus aureus virulence factors, but little is known about their immunogenicity during infection. Here, additional insight is generated. METHODS: Serum samples from 206 S. aureus-infected patients and 201 hospital-admitted control subjects we

  19. The cell surface proteome of Staphylococcus aureus

    NARCIS (Netherlands)

    Dreisbach, Annette; van Dijl, Jan Maarten; Buist, Girbe

    2011-01-01

    The Gram-positive bacterium Staphylococcus aureus is a wide spread opportunistic pathogen that can cause a range of life-threatening diseases. To obtain a better understanding of the global mechanisms for pathogenesis and to identify novel targets for therapeutic interventions, the S. aureus proteom

  20. Population structure of Staphylococcus aureus in China

    NARCIS (Netherlands)

    Yan, Xiaomei

    2015-01-01

    The present PhD research was aimed at analysing the population structure of Staphylococcus aureus in China. Between 2000 and 2005 we found that patients from a single Chinese hospital showed increasing trends in antimicrobial resistance. Among methicillin-resistant S. aureus (MRSA), resistance again

  1. Economic model for QoS guarantee on the Internet

    Science.gov (United States)

    Zhang, Chi; Wei, Jiaolong

    2001-09-01

    This paper describes a QoS guarantee architecture suited for best-effort environments, based on ideas from microeconomics and non-cooperative game theory. First, an analytic model is developed for the study of the resource allocation in the Internet. Then we show that with a simple pricing mechanism (from network implementation and users' points-of-view), we were able to provide QoS guarantee at per flow level without resource allocation or complicated scheduling mechanisms or maintaining per flow state in the core network. Unlike the previous work on this area, we extend the basic model to support inelastic applications which require minimum bandwidth guarantees for a given time period by introducing derivative market.

  2. Unpower aerocraft augmented state feedback tracking guaranteed cost control

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Aimed at designing the unpower aerocraft attitude control system in a simple and practical way,the guaranteed cost control is adopted.To eliminate the steady-error,a novel tracking control approach-augmented state feedback tracking guaranteed cost control is proposed.Firstly,the unpower aerocraft is modeled as a linear system with norm bounded parameter uncertain,then the linear matrix inequality based state feedback gnaranteed cost control law is combined with the augmented state feedback tracking control from a new point of view.The sufficient condition of the existence of the augmented state feedback tracking guaranteed cost control is derived and converted to the feasible problem of the linear matrix inequality.Finally,the proposed approach is applied to a specified unpower aerocraft.The six dimensions of freedom simulation results show that the proposed approach is effective and feasible.

  3. Guarantees of Augmented Trace Norm Models in Tensor Recovery

    OpenAIRE

    Shi, Ziqiang; Han, Jiqing; Zheng, Tieran; Deng, Shiwen; Li, Ji

    2012-01-01

    This paper studies the recovery guarantees of the models of minimizing $\\|\\mathcal{X}\\|_*+\\frac{1}{2\\alpha}\\|\\mathcal{X}\\|_F^2$ where $\\mathcal{X}$ is a tensor and $\\|\\mathcal{X}\\|_*$ and $\\|\\mathcal{X}\\|_F$ are the trace and Frobenius norm of respectively. We show that they can efficiently recover low-rank tensors. In particular, they enjoy exact guarantees similar to those known for minimizing $\\|\\mathcal{X}\\|_*$ under the conditions on the sensing operator such as its null-space property, ...

  4. AN ADAPTIVE AQM TO PROVIDE DELAY GUARANTEES FOR DIFFERENTIATED SERVICES

    Institute of Scientific and Technical Information of China (English)

    Yang Zongkai; Liu Wei; He Jianhua; Chou Chuntung

    2005-01-01

    RIO(RED with IN and OUT) is the primary queue management mechanism proposed for assured forwarding in the DiffServ (Differentiated Service) framework. Although RIO can generally provide bandwidth guarantees, its queuing delay is sensitive to the traffic load. This paper presents a qualitative explanation for its origin. As a solution, an Adaptive RIO for Delay (ARIO-D) is proposed to provide guaranteed delay for multimedia traffic. Simulation results show that by trading loss for delay, ARIO-D can effectively improve the robustness of RIO under different and dynamic traffic, and provide stable and differentiated performance of queuing delay without any degradation in performance of throughput.

  5. Robust levitation control for maglev systems with guaranteed bounded airgap.

    Science.gov (United States)

    Xu, Jinquan; Chen, Ye-Hwa; Guo, Hong

    2015-11-01

    The robust control design problem for the levitation control of a nonlinear uncertain maglev system is considered. The uncertainty is (possibly) fast time-varying. The system has magnitude limitation on the airgap between the suspended chassis and the guideway in order to prevent undesirable contact. Furthermore, the (global) matching condition is not satisfied. After a three-step state transformation, a robust control scheme for the maglev vehicle is proposed, which is able to guarantee the uniform boundedness and uniform ultimate boundedness of the system, regardless of the uncertainty. The magnitude limitation of the airgap is guaranteed, regardless of the uncertainty. PMID:26524957

  6. 13 CFR 115.30 - Submission of Surety's guarantee application.

    Science.gov (United States)

    2010-01-01

    ... days, or in the case of a bonding line, within 15 business days (see § 115.33(d)(2)) after Execution of... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Submission of Surety's guarantee application. 115.30 Section 115.30 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SURETY...

  7. 7 CFR 1493.260 - Facility payment guarantee.

    Science.gov (United States)

    2010-01-01

    ...; and (2) Cost means actual amount paid by the exporter for the services in an arms-length transaction; or in the absence of an arms-length transaction, the fair market value of the services at the time... facility payment guarantee may result in an increase to the exposure fee. Requests made by the exporter...

  8. 12 CFR 370.3 - Debt Guarantee Program.

    Science.gov (United States)

    2010-01-01

    ... convertible debt to be issued, (C) The mandatory conversion date, (D) The conversion rate (i.e., the total... exchange rate in effect on the date that the debt is funded. (c) Calculation and reporting responsibility... guarantee expires on the earliest of the mandatory conversion date for mandatory convertible debt,...

  9. Identifying effective guaranteed connections in a multimodal public transport network

    NARCIS (Netherlands)

    Sparing, D.; Goverde, R.M.P.

    2012-01-01

    Minimizing transfer waiting time is important in making public transport networks more attractive. A guaranteed transfer, with the departing vehicle waiting on moderately delayed arriving vehicles at a transfer node, is an effective way to reduce waiting times at transfers between low frequency publ

  10. Identifying effective guaranteed connections in a multimodal network

    NARCIS (Netherlands)

    Sparing, D.; Goverde, R.M.P.

    2012-01-01

    Minimizing transfer waiting time is important in making public transport networks more attractive. A guaranteed transfer, with the departing vehicle waiting on moderately delayed arriving vehicles at a transfer node, is an effective way to reduce waiting times at transfers between low frequency publ

  11. Independence:Guarantee to Emerson’s Individualism

    Institute of Scientific and Technical Information of China (English)

    Jiang; Jingxin

    2015-01-01

    Emerson established a systematic philosophy of individualism at his early stage.The basic three elements of individualism described in Emerson’s works are:freedom,selfidentity and independence to others.Independence,serves as the guarantee to the realization of individualism.

  12. Independence:Guarantee to Emerson’s Individualism

    Institute of Scientific and Technical Information of China (English)

    江晶鑫

    2015-01-01

    Emerson established a systematic philosophy of individualism at his early stage.The basic three elements of individualism described in Emerson's works are:freedom,self-identity and independence to others.Independence,serves as the guarantee to the realization of individualism.

  13. 12 CFR 370.12 - Payment on the guarantee.

    Science.gov (United States)

    2010-01-01

    ... transaction deposit accounts in such entities will be paid in accordance with 12 U.S.C. 1821(f) and 12 CFR... satisfaction. Payment under paragraph (b)(2) of this section shall constitute, to the extent of payments made, satisfaction of all FDIC obligations under the debt guarantee program with respect to that debtholder...

  14. 40 CFR 280.106 - Local government guarantee.

    Science.gov (United States)

    2010-07-01

    ... exceeds [select one: the local government bond rating test requirements of 40 CFR part 280.104, the local government financial test requirements of 40 part CFR 280.105, the local government fund under 40 CFR part... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Local government guarantee....

  15. Genomic Analysis of Companion Rabbit Staphylococcus aureus.

    Science.gov (United States)

    Holmes, Mark A; Harrison, Ewan M; Fisher, Elizabeth A; Graham, Elizabeth M; Parkhill, Julian; Foster, Geoffrey; Paterson, Gavin K

    2016-01-01

    In addition to being an important human pathogen, Staphylococcus aureus is able to cause a variety of infections in numerous other host species. While the S. aureus strains causing infection in several of these hosts have been well characterised, this is not the case for companion rabbits (Oryctolagus cuniculus), where little data are available on S. aureus strains from this host. To address this deficiency we have performed antimicrobial susceptibility testing and genome sequencing on a collection of S. aureus isolates from companion rabbits. The findings show a diverse S. aureus population is able to cause infection in this host, and while antimicrobial resistance was uncommon, the isolates possess a range of known and putative virulence factors consistent with a diverse clinical presentation in companion rabbits including severe abscesses. We additionally show that companion rabbit isolates carry polymorphisms within dltB as described as underlying host-adaption of S. aureus to farmed rabbits. The availability of S. aureus genome sequences from companion rabbits provides an important aid to understanding the pathogenesis of disease in this host and in the clinical management and surveillance of these infections. PMID:26963381

  16. 21 CFR 866.3700 - Staphylococcus aureus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Staphylococcus aureus serological reagents. 866... Staphylococcus aureus serological reagents. (a) Identification. Staphylococcus aureus serological reagents are... epidemiological information on these diseases. Certain strains of Staphylococcus aureus produce an...

  17. Exfoliative Toxins of Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Michal Bukowski

    2010-05-01

    Full Text Available Staphylococcus aureus is an important pathogen of humans and livestock. It causes a diverse array of diseases, ranging from relatively harmless localized skin infections to life-threatening systemic conditions. Among multiple virulence factors, staphylococci secrete several exotoxins directly associated with particular disease symptoms. These include toxic shock syndrome toxin 1 (TSST-1, enterotoxins, and exfoliative toxins (ETs. The latter are particularly interesting as the sole agents responsible for staphylococcal scalded skin syndrome (SSSS, a disease predominantly affecting infants and characterized by the loss of superficial skin layers, dehydration, and secondary infections. The molecular basis of the clinical symptoms of SSSS is well understood. ETs are serine proteases with high substrate specificity, which selectively recognize and hydrolyze desmosomal proteins in the skin. The fascinating road leading to the discovery of ETs as the agents responsible for SSSS and the characterization of the molecular mechanism of their action, including recent advances in the field, are reviewed in this article.

  18. [Staphylococcus aureus and antibiotic resistance].

    Science.gov (United States)

    Sancak, Banu

    2011-07-01

    After the report of first case of methicillin-resistant Staphylococcus aureus (MRSA) in 1961, MRSA become a major problem worldwide. Over the last decade MRSA strains have emerged as serious pathogens in nosocomial and community settings. Glycopeptides (vancomycin and teicoplanin) are still the current mainstay of therapy for infections caused by MRSA. In the last decade dramatic changes have occurred in the epidemiology of MRSA infections. The isolates with reduced susceptibility and in vitro resistance to vancomycin have emerged. Recently, therapeutic alternatives such as quinupristin/dalfopristin, linezolid, tigecycline and daptomycin have been introduced into clinical practice for treating MRSA infections. Nevertheless, these drugs are only approved for certain indication and resistance has already been reported. In this review, the new information on novel drugs for treating MRSA infections and the resistance mechanisms of these drugs were discussed. PMID:21935792

  19. The Heme Sensor System of Staphylococcus aureus

    OpenAIRE

    Stauff, Devin L; Skaar, Eric P.

    2009-01-01

    The important human pathogen Staphylococcus aureus is able to satisfy its nutrient iron requirement by acquiring heme from host hemoglobin in the context of infection. However, heme acquisition exposes S. aureus to heme toxicity. In order to detect the presence of toxic levels of exogenous heme, S. aureus is able to sense heme through the heme sensing system (HssRS) two-component system. Upon sensing heme, HssRS directly regulates the expression of the heme-regulated ABC transporter HrtAB, wh...

  20. Insights into tRNA-Dependent Amidotransferase Evolution and Catalysis from the Structure of the Aquifex aeolicus Enzyme

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Jing; Bu, Weishu; Sheppard, Kelly; Kitabatake, Makoto; Kwon, Suk-Tae; Söll, Dieter; Smith, Janet L.; (Yale); (Michigan); (Kyoto)

    2010-08-17

    Many bacteria form Gln-tRNA{sup Gln} and Asn-tRNA{sup ASN} by conversion of the misacylated Glu-tRNA{sup Gln} and Asp-tRNA{sup ASN} species catalyzed by the GatCAB amidotransferase in the presence of ATP and an amide donor (glutamine or asparagine). Here, we report the crystal structures of GatCAB from the hyperthermophilic bacterium Aquifex aeolicus, complexed with glutamine, asparagine, aspartate, ADP, or ATP. In contrast to the Staphylococcus aureus GatCAB, the A. aeolicus enzyme formed acyl-enzyme intermediates with either glutamine or asparagine, in line with the equally facile use by the amidotransferase of these amino acids as amide donors in the transamidation reaction. A water-filled ammonia channel is open throughout the length of the A. aeolicus GatCAB from the GatA active site to the synthetase catalytic pocket in the B-subunit. A non-catalytic Zn{sup 2+} site in the A. aeolicus GatB stabilizes subunit contacts and the ammonia channel. Judged from sequence conservation in the known GatCAB sequences, the Zn{sup 2+} binding motif was likely present in the primordial GatB/E, but became lost in certain lineages (e.g., S. aureus GatB). Two divalent metal binding sites, one permanent and the other transient, are present in the catalytic pocket of the A. aeolicus GatB. The two sites enable GatCAB to first phosphorylate the misacylated tRNA substrate and then amidate the activated intermediate to form the cognate products, Gln-tRNA{sup Gln} or Asn-tRNA{sup ASN}.

  1. Laboratory Maintenance of Methicillin-Resistant Staphylococcus aureus (MRSA)

    OpenAIRE

    Nicholas P Vitko; Richardson, Anthony R.

    2013-01-01

    Staphylococcus aureus is an important bacterial pathogen in the hospital and community settings, especially Staphylococcus aureus clones that exhibit methicillin-resistance (MRSA). Many strains of S. aureus are utilized in the laboratory, underscoring the genetic differences inherent in clinical isolates. S. aureus grows quickly at 37°C with aeration in rich media (e.g. BHI) and exhibits a preference for glycolytic carbon sources. Furthermore, S. aureus has a gold pigmentation, exhibits β-hem...

  2. Mastite com lesões sistêmicas por Staphylococus aureus subesp. aureus em coelhos

    OpenAIRE

    Traverso Sandra Davi; Cunha Leonardo da; Fernandes Joaquim César Teixeira; Loretti Alexandre Paulino; Rhoden Adriana; Wunder Jr. Elsio; Driemeier David

    2003-01-01

    Em uma criação composta por 1800 coelhos, 33% das matrizes apresentaram mastite e lesões cutâneas crostosas e purulentas. Estes animais apresentavam-se entre 10 a- 12 meses de idade e em segunda parição. Quinze coelhos afetados foram sacrificados e necropsiados. Na necropsia, além das lesões cutâneas haviam microabscessos em diversos órgãos. Das amostras coletadas isolou-se Staphylococcus aureus subesp. aureus. S. aureus subesp. aureus também foi isolado de "swab" nasal coletado do tratador e...

  3. Staphylococcus aureus spa type t437

    DEFF Research Database (Denmark)

    Glasner, C; Pluister, G; Westh, H;

    2015-01-01

    Methicillin-resistant Staphylococcus aureus (MRSA) belonging to the multilocus sequence type clonal complex 59 (MLST CC59) is the predominant community-associated MRSA clone in Asia. This clone, which is primarily linked with the spa type t437, has so far only been reported in low numbers among...... large epidemiological studies in Europe. Nevertheless, the overall numbers identified in some Northern European reference laboratories have increased during the past decade. To determine whether the S. aureus t437 clone is present in other European countries, and to assess its genetic diversity across...... Europe, we analysed 147 S. aureus t437 isolates from 11 European countries collected over a period of 11 years using multiple locus variable number tandem repeat fingerprinting/analysis (MLVF/MLVA) and MLST. Additionally 16 S. aureus t437 isolates from healthy carriers and patients from China were...

  4. PARAMETER KINETIKA INAKTIVASI TERMAL DAN ISOLASI Staphylococcus aureus PADA MINUMAN DARI GEL CINCAU HIJAU DAN ROSELA [Thermal Inactivation Kinetics Parameter and Isolation of Staphylococcus aureus on Drink from Green Grass Jelly and Roselle

    Directory of Open Access Journals (Sweden)

    Eko Hari Purnomo

    2015-07-01

    Full Text Available Information about heat resistance (D and z values of target bacteria is needed for the thermal process design on drink from Green Grass Jelly (Premna oblongifolia Merr. and Roselle (Hibiscus sabdariffa L., so it can guarantee quality and safety of the product. The objectives of this research were to isolate Staphylococcus aureus from commercial green grass jelly and to determine the D and z values of Staphylococcus aureus (from commercial product and standard clinical isolate ATCC 25923 on heating menstruum of green grass jelly and roselle. Isolation of S. aureus was done by inoculation in selective medium, D and z values of Staphylococcus aureus were assessed by heating at constant temperatures of 57, 53, 49, and 45°C during the time interval 2.5, 5, 10, and 15 minutes. The results showed that one isolate (Isolate A gave positive response of Staphylococcus aureus in isolation tests and had similar percentage with the reference culture of 41.8% using the API Staph Kit. Heat resistance of Staphylococcus aureus (represented as D value isolated from green grass jelly at constant heating temperature of D45, D49, D53 and D57 were 32.3, 17.9, 4.6, and 1.5 minutes. On the other hand, D value of isolates ATCC 25923 (standard clinical isolate at constant heating temperature of D45, D49, D53 and D57 were 18.5, 6.8, 2.9, and 1.4 minutes. The z value of isolates A and ATCC 25923 were 8.8°C and 10.8°C. Smaller z value of isolate A showed that pasteurization process can be accelerated and optimized with increasing the temperature slightly but has the same lethality effect.

  5. The Staphylococcus aureus “superbug”

    OpenAIRE

    FOSTER, TIMOTHY JAMES

    2004-01-01

    PUBLISHED There has been some debate about the disease-invoking potential of Staphylococcus aureus strains and whether invasive disease is associated with particularly virulent genotypes, or "superbugs." A study in this issue of the JCI describes the genotyping of a large collection of nonclinical, commensal S. aureus strains from healthy individuals in a Dutch population. Extensive study of their genetic relatedness by amplified restriction fragment typing and comparison with strains that...

  6. Transmissible mupirocin resistance in Staphylococcus aureus.

    OpenAIRE

    Rahman, M.; Noble, W. C.; Cookson, B

    1989-01-01

    The spread of two strains of Staphylococcus aureus with high level resistance to mupirocin is described. The resistance proved to be easily transferred to other S. aureus strains by filter mating experiments and on the skin of mice. No plasmid band corresponding to the resistance could be demonstrated by agarose gel electrophoresis or by caesium chloride gradient centrifugation but cleavage of 'chromosomal' DNA from resistant recipients showed bright bands of DNA absent from sensitive controls.

  7. SHALE: An Efficient Algorithm for Allocation of Guaranteed Display Advertising

    CERN Document Server

    Bharadwaj, Vijay; Ma, Wenjing; Nagarajan, Chandrashekhar; Tomlin, John; Vassilvitskii, Sergei; Vee, Erik; Yang, Jian

    2012-01-01

    Motivated by the problem of optimizing allocation in guaranteed display advertising, we develop an efficient, lightweight method of generating a compact {\\em allocation plan} that can be used to guide ad server decisions. The plan itself uses just O(1) state per guaranteed contract, is robust to noise, and allows us to serve (provably) nearly optimally. The optimization method we develop is scalable, with a small in-memory footprint, and working in linear time per iteration. It is also "stop-anytime", meaning that time-critical applications can stop early and still get a good serving solution. Thus, it is particularly useful for optimizing the large problems arising in the context of display advertising. We demonstrate the effectiveness of our algorithm using actual Yahoo! data.

  8. Mobile intelligent agent entity model towards QoS guarantee

    Institute of Scientific and Technical Information of China (English)

    LI Jie; WANG Ru-chuan; BIAN Zhen-gai

    2006-01-01

    Implementing a flexible configuration of the QoS parameter in a distributed computing network has become a problem due to the weak scalability of current approaches.In an effort to solve this problem,an inner basic model of an intelligent agent (IA) is presented.The IA functionality was extended by introducing a primarily mobile agent.A QoS guarantee scheme was subsequently designed and implemented based on the model as well.By utilizing the proposed scheme,the IA can sense,predict and configure the data flow traffic.Since the communicating ability was considered and provided,the competition among different devices could be eliminated effectively and the global traffic can be optimized.The results of the simulations have shown that the proposed model can provide a QoS guarantee.

  9. Automated Assume-Guarantee Reasoning by Abstraction Refinement

    Science.gov (United States)

    Pasareanu, Corina S.; Giannakopoulous, Dimitra; Glannakopoulou, Dimitra

    2008-01-01

    Current automated approaches for compositional model checking in the assume-guarantee style are based on learning of assumptions as deterministic automata. We propose an alternative approach based on abstraction refinement. Our new method computes the assumptions for the assume-guarantee rules as conservative and not necessarily deterministic abstractions of some of the components, and refines those abstractions using counter-examples obtained from model checking them together with the other components. Our approach also exploits the alphabets of the interfaces between components and performs iterative refinement of those alphabets as well as of the abstractions. We show experimentally that our preliminary implementation of the proposed alternative achieves similar or better performance than a previous learning-based implementation.

  10. To help allies, send security guarantees, not nuclear bombs

    OpenAIRE

    Fuhrmann, Matthew; Sechser , Todd S.

    2014-01-01

    Despite the end of the Cold War more than two decades ago, the U.S. still deploys nuclear weapons in several countries. But does deploying these weapons act as a deterrent to conflict, making these countries safer? In new research which analyses the effects of such nuclear deployments, Matthew Fuhrmann and Todd S. Sechser find that their presence does little, when compared with alliance guarantees. They write that having an alliance with a nuclear armed ally is actually more effective in prev...

  11. Traffic Scheduling in WDM Passive Optical Network with Delay Guarantee

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    WDM passive optical network becomes more favorable as the required bandwidth increases, but currently few media access control algorithms adapted to WDM access network. This paper presented a new scheduling algorithm for bandwidth sharing in WDM passive optical networks, which provides per-flow delay guarantee and supports variable-length packets scheduling. Through theoretical analysis and simulation, the end-to-end delay bound and throughput fairness of the algorithm was demonstrated.

  12. Research on optimal guaranteed cost control of flexible spacecraft

    Institute of Scientific and Technical Information of China (English)

    Wang Qingchao; Cai Peng

    2008-01-01

    This article is concerned with the modeling and control problems of the flexible spacecraft.First,the state observer is designed to estimate the vibration mode on the basis of free vibration models.Then,an optimal guaranteed cost controller is proposed to stabilize system attitude and damp the vibration of the flexible beam at the same time.Numerical simulation examples show the feasibility and validity of the proposed method.

  13. Money-Back Guarantees: Helping the Low-Quality Retailer

    OpenAIRE

    Bruce McWilliams

    2012-01-01

    Existing literature, based on signaling theory, suggests that money-back guarantees (MBGs) will be utilized by high-quality firms, where high quality is defined as a low likelihood of product return. However, in today's world, MBGs are ubiquitous among major retailers, even when the likelihood of product return varies greatly between them. To understand this phenomenon, we explore a competitive environment between high- and low-quality retailers where consumers are fully informed and risk neu...

  14. GEARSHIFT: Guaranteeing availability requirements in SLAs using hybrid fault tolerance

    OpenAIRE

    Gonzalez, Andres Javier; Helvik, Bjarne Emil; Tiwari, Prakriti; Denis, Becker; Wittner, Otto Jonassen

    2015-01-01

    The dependability of ICT systems is vital for today's society. However, operational systems are not fault free. Providers and customers have to define clear availability requirements and penalties on the delivered services by using SLAs. Fulfilling the stipulated availability may be expensive. The lack of mechanisms that allow a fine control of the SLA risk may lead to over-dimension the provided resources. Therefore, a relevant question for ICT service providers is: How to guarantee the SLA ...

  15. STF: PROVIDE RATE GUARANTEES IN INPUT-QUEUED SWITCH

    Institute of Scientific and Technical Information of China (English)

    Wang Wenjie; Wei Gang

    2002-01-01

    A QoS-aware input-queued scheduling algorithm, called Smallest Timestamp First (STF), is proposed, which is improved upon iSLIP and can allocate bandwidth among inputs sharing a common output based on their reservation by assigning suitable finishing timestamps to contending cells. STF can also provide isolation between flows that share a common output link.Misbehaving flows will be restricted to guarantee the behaving flows' bandwidth. Simulatious prove the feasibility of our algorithm.

  16. Information Extraction on the Web with Credibility Guarantee

    OpenAIRE

    Nguyen, Thanh Tam

    2015-01-01

    The Web became the central medium for valuable sources of information extraction applications. However, such user-generated resources are often plagued by inaccuracies and misinformation due to the inherent openness and uncertainty of the Web. In this work we study the problem of extracting structured information out of Web data with a credibility guarantee. The ultimate goal is that not only the structured information should be extracted as much as possible but also its credibility is high. ...

  17. Providing Credit Guarantee Scheme in Agriculture of Ukraine

    OpenAIRE

    Olena Oliynyk

    2013-01-01

    Small and medium-size businesses, despite their significant contribution to agricultural production in Ukraine, do have limited access to financing. Existing public support mechanisms (interest rate subsidies, leasing programs) do not solve the problem of these categories of enterprises access to credit resources. Introduction of credit guarantee scheme is a more effective mechanism to support small and medium-size businesses. As international experience shows, it is a market mechanism - the ...

  18. Guaranteed Minimum Price Contracts for Some, an Insurance for Others?

    OpenAIRE

    Chambolle, Claire; Poret, Sylvaine,

    2008-01-01

    This paper analyzes the impact of guaranteed minimum price contracts between sub-groups of farmers and a fair trade manufacturer on the spot market price. We focus on the fair trade concept in the coffee supply chain as an example. We analyze a three level vertical chain gathering perfectly competitive farmers upstream who offer their raw product to manufacturers who then sell finished products to a downstream retailer. Without fair trade, all the raw product is sold on the spot market. When ...

  19. Assume-Guarantee Synthesis for Digital Contract Signing

    CERN Document Server

    Chatterjee, Krishnendu

    2010-01-01

    We study the automatic synthesis of fair non-repudiation protocols, a class of fair exchange protocols, used for digital contract signing. First, we show how to specify the objectives of the participating agents and the trusted third party (TTP) as path formulas in LTL and prove that the satisfaction of these objectives imply fairness and abuse-freeness; properties required of fair exchange protocols. We then show that weak (co-operative) co-synthesis and classical (strictly competitive) co-synthesis fail, whereas assume-guarantee synthesis (AGS) succeeds. We demonstrate the success of assume-guarantee synthesis as follows: (a) any solution of assume-guarantee synthesis is attack-free; no subset of participants can violate the objectives of the other participants; (b) the Asokan-Shoup-Waidner (ASW) certified mail protocol that has known vulnerabilities is not a solution of AGS; (c) The Garay-Jakobsson-MacKenzie (GJM) protocol, while fair and abuse-free, is not attack-free by our definition and is hence not a ...

  20. Carotenoid Formation by Staphylococcus aureus

    Science.gov (United States)

    Hammond, Ray K.; White, David C.

    1970-01-01

    The carotenoid pigments of Staphylococcus aureus U-71 were identified as phytoene; ζ-carotene; δ-carotene; phytofluenol; a phytofluenol-like carotenoid, rubixanthin; and three rubixanthin-like carotenoids after extraction, saponification, chromatographic separation, and determination of their absorption spectra. There was no evidence of carotenoid esters or glycoside ethers in the extract before saponification. During the aerobic growth cycle the total carotenoids increased from 45 to 1,000 nmoles per g (dry weight), with the greatest increases in the polar, hydroxylated carotenoids. During the anaerobic growth cycle, the total carotenoids increased from 20 nmoles per g (dry weight) to 80 nmoles per g (dry weight), and only traces of the polar carotenoids were formed. Light had no effect on carotenoid synthesis. About 0.14% of the mevalonate-2-14C added to the culture was incorporated into the carotenoids during each bacterial doubling. The total carotenoids did not lose radioactivity when grown in the absence of 14C for 2.5 bacterial doublings. The total carotenoids did not lose radioactivity when grown in the absence of 14C for 2.5 bacterial doublings. The incorporation and turnover of 14C indicated the carotenes were sequentially desaturated and hydroxylated to form the polar carotenoids. PMID:5423369

  1. Complete Genome Sequence of the Quality Control Strain Staphylococcus aureus subsp. aureus ATCC 25923.

    Science.gov (United States)

    Treangen, Todd J; Maybank, Rosslyn A; Enke, Sana; Friss, Mary Beth; Diviak, Lynn F; Karaolis, David K R; Koren, Sergey; Ondov, Brian; Phillippy, Adam M; Bergman, Nicholas H; Rosovitz, M J

    2014-01-01

    Staphylococcus aureus subsp. aureus ATCC 25923 is commonly used as a control strain for susceptibility testing to antibiotics and as a quality control strain for commercial products. We present the completed genome sequence for the strain, consisting of the chromosome and a 27.5-kb plasmid. PMID:25377701

  2. Complete Genome Sequence of the Quality Control Strain Staphylococcus aureus subsp. aureus ATCC 25923

    OpenAIRE

    Todd J. Treangen; Maybank, Rosslyn A.; Enke, Sana; Friss, Mary Beth; Diviak, Lynn F.; Karaolis, David K. R.; Koren, Sergey; Ondov, Brian; Phillippy, Adam M.; Bergman, Nicholas H.; Rosovitz, M. J.

    2014-01-01

    Staphylococcus aureus subsp. aureus ATCC 25923 is commonly used as a control strain for susceptibility testing to antibiotics and as a quality control strain for commercial products. We present the completed genome sequence for the strain, consisting of the chromosome and a 27.5-kb plasmid.

  3. Potassium Uptake Modulates Staphylococcus aureus Metabolism.

    Science.gov (United States)

    Gries, Casey M; Sadykov, Marat R; Bulock, Logan L; Chaudhari, Sujata S; Thomas, Vinai C; Bose, Jeffrey L; Bayles, Kenneth W

    2016-01-01

    As a leading cause of community-associated and nosocomial infections, Staphylococcus aureus requires sophisticated mechanisms that function to maintain cellular homeostasis in response to its exposure to changing environmental conditions. The adaptation to stress and maintenance of homeostasis depend largely on membrane activity, including supporting electrochemical gradients and synthesis of ATP. This is largely achieved through potassium (K(+)) transport, which plays an essential role in maintaining chemiosmotic homeostasis, affects antimicrobial resistance, and contributes to fitness in vivo. Here, we report that S. aureus Ktr-mediated K(+) uptake is necessary for maintaining cytoplasmic pH and the establishment of a proton motive force. Metabolite analyses revealed that K(+) deficiency affects both metabolic and energy states of S. aureus by impairing oxidative phosphorylation and directing carbon flux toward substrate-level phosphorylation. Taken together, these results underline the importance of K(+) uptake in maintaining essential components of S. aureus metabolism. IMPORTANCE Previous studies describing mechanisms for K(+) uptake in S. aureus revealed that the Ktr-mediated K(+) transport system was required for normal growth under alkaline conditions but not under neutral or acidic conditions. This work focuses on the effect of K(+) uptake on S. aureus metabolism, including intracellular pH and carbon flux, and is the first to utilize a pH-dependent green fluorescent protein (GFP) to measure S. aureus cytoplasmic pH. These studies highlight the role of K(+) uptake in supporting proton efflux under alkaline conditions and uncover a critical role for K(+) uptake in establishing efficient carbon utilization. PMID:27340697

  4. 76 FR 46832 - Notice of Proposed Information Collection: Loan Guarantee Recovery Fund; Comment Request

    Science.gov (United States)

    2011-08-03

    ... URBAN DEVELOPMENT Notice of Proposed Information Collection: Loan Guarantee Recovery Fund; Comment.... This Notice also lists the following information: Title of Proposal: Loan Guarantee Recovery Fund. OMB...: Notice. SUMMARY: The proposed information collection requirement described below will be submitted to...

  5. 76 FR 71058 - Notice of Submission of Proposed Information Collection to OMB Loan Guarantee Recovery Fund...

    Science.gov (United States)

    2011-11-16

    ... URBAN DEVELOPMENT Notice of Submission of Proposed Information Collection to OMB Loan Guarantee Recovery... responses. This Notice Also Lists the Following Information Title of Proposal: Loan Guarantee Recovery Fund... Information Officer, HUD. ACTION: Notice. SUMMARY: The proposed information collection requirement...

  6. The Impact of a State Bond Guarantee on State Credit Markets and Individual Municipalities

    OpenAIRE

    Hsueh, L. Paul; Kidwell, David S.

    1988-01-01

    Examines the overall effect of a state bond guarantee on state credit markets and individual municipalities. Analyzes the Texas School Bond Guarantee program and it's impact on the borrowing cost of school districts and other municipalities within the state.

  7. GUARANTEED COST CONTROL OF CONTINUOUS-TIME UNCERTAIN SINGULAR SYSTEM WITH BOTH STATE AND INPUT DELAYS

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    The guaranteed cost control problem for a continuous-time uncertain singular system with state and control delays, and a given quadratic cost function is studied in this paper. Sufficient conditions for the existence of the guaranteed cost controller are derived based on the linear inequality (LMI) approach. A parameterized characterization of the guaranteed cost laws is given in terms of the feasible solutions to a certain LMI, and the cost function of guaranteed cost controller exists an upper bound.

  8. Valuing Guaranteed Minimum Death Benefit Options in Variable Annuities Under a Benchmark Approach

    OpenAIRE

    Marquardt, T.; Eckhard Platen; S. Jaschke

    2008-01-01

    Variable annuities (VAs) represent a marked change from earlier life products in the guarantees that they offer and it is no longer possible to manage the risks of these liabilities using traditional actuarial methods. Thinking about guarantees as options suggests applying risk neutral pricing in order to value the embedded guarantees, such as guaranteed minimum death benefits (GMDBs). However, due to the long maturities of contracts, stochastic volatility and many other reasons, VA markets a...

  9. Efficient Vessel Tracking  with Accuracy Guarantees

    DEFF Research Database (Denmark)

    Redoutey, Martin; Scotti, Eric; Jensen, Christian Søndergaard;

    2008-01-01

    Safety and security are top concerns in maritime navigation, particularly as maritime traffic continues to grow and as crew sizes are reduced. The Automatic Identification System (AIS) plays a key role in regard to these concerns. This system, whose objective is in part to identify and locate...... vessels, transmits location-related information from vessels to ground stations that are part of a so-called Vessel Traffic Service (VTS), thus enabling these to track the movements of the vessels. This paper presents techniques that improve the existing AIS by offering better and guaranteed tracking...

  10. Organic Law Of Judicial Guarantees And Constitutional Control

    Directory of Open Access Journals (Sweden)

    Ernesto López Freire

    2013-01-01

    Full Text Available This paper demonstrates the various unconstitutional and fallacies of the Organic Law of Judicial guarantees and Constitutional Control. For that, there will be a comprehensive collation between the mentioned law and the Constitution of the Republic of Ecuador and force. Through this analysis shows a lack of knowledge of Ecuadorian law or legal science by their authors. This study elucidated, inter alia, the inconsistencies in matters relating to the interpretation of constitutional provisions, full compensation, material and integral; challenge administrative acts, judicial unit.

  11. On guaranteed cost control of linear systems with input saturation

    Institute of Scientific and Technical Information of China (English)

    FU Min-yue

    2007-01-01

    This work studies the problem of control design for linear systems with input saturation. It is well known that integral quadratic constraints (IQC) can be used to describe input saturation and that the use of IQC in analysis can lead to less conservative performance bound and larger domain of attraction. In this work, it is shown that a class of commonly used IQCs may not help in control synthesis. That is, the use of these IQCs does not enlarge the guaranteed domain of performance for synthesis.

  12. The Minimum Price Guarantee Policy before and after Real Plan

    Directory of Open Access Journals (Sweden)

    Jackelline Favro

    2014-12-01

    Full Text Available This study aims to analyze the performance of the Minimum Price Guarantee Policy - MPGPin Brazil for the main agricultural products, during the 1980s and 1990s, and especially the post-Real Plan period. The research has a bibliographic and descriptive nature. The main results showed that the MPGP remains an important tool for ensuring income to farmers and the supply of selected crops in the analyzed period, but there was a reduction of public action for the new MPGP and new private instruments has revealed efficiency

  13. 7 CFR 457.128 - Guaranteed production plan of fresh market tomato crop insurance provisions.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Guaranteed production plan of fresh market tomato crop... § 457.128 Guaranteed production plan of fresh market tomato crop insurance provisions. The Guaranteed Production Plan of Fresh Market Tomato Crop Insurance FCIC Policies Department of Agriculture Federal...

  14. 7 CFR 1786.202 - Prepayment and refinancing of RUS guaranteed FFB loans.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 12 2010-01-01 2010-01-01 false Prepayment and refinancing of RUS guaranteed FFB...) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE (CONTINUED) PREPAYMENT OF RUS GUARANTEED AND INSURED LOANS TO ELECTRIC AND TELEPHONE BORROWERS Refinancing and Prepayment of RUS Guaranteed FFB...

  15. 7 CFR 1786.35 - Loss, theft, destruction, mutilation, or defacement of RUS guarantee.

    Science.gov (United States)

    2010-01-01

    ... of RUS guarantee. 1786.35 Section 1786.35 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE (CONTINUED) PREPAYMENT OF RUS GUARANTEED AND INSURED LOANS TO ELECTRIC AND TELEPHONE BORROWERS Prepayment of RUS Guaranteed Federal Financing...

  16. Genomics of Natural Populations of Staphylococcus aureus.

    Science.gov (United States)

    Fitzgerald, J Ross; Holden, Matthew T G

    2016-09-01

    Staphylococcus aureus is a major human pathogen and an important cause of livestock infections. The first S. aureus genomes to be published, 15 years ago, provided the first view of genome structure and gene content. Since then, thousands of genomes from a wide array of strains from different sources have been sequenced. Comparison of these sequences has resulted in broad insights into population structure, bacterial evolution, clone emergence and expansion, and the molecular basis of niche adaptation. Furthermore, this information is now being applied clinically in outbreak investigations to inform infection control measures and to determine appropriate treatment regimens. In this review, we summarize some of the broad insights into S. aureus biology gained from the analysis of genomes and discuss future directions and opportunities in this dynamic field of research. PMID:27482738

  17. Genomics of Natural Populations of Staphylococcus aureus.

    Science.gov (United States)

    Fitzgerald, J Ross; Holden, Matthew T G

    2016-09-01

    Staphylococcus aureus is a major human pathogen and an important cause of livestock infections. The first S. aureus genomes to be published, 15 years ago, provided the first view of genome structure and gene content. Since then, thousands of genomes from a wide array of strains from different sources have been sequenced. Comparison of these sequences has resulted in broad insights into population structure, bacterial evolution, clone emergence and expansion, and the molecular basis of niche adaptation. Furthermore, this information is now being applied clinically in outbreak investigations to inform infection control measures and to determine appropriate treatment regimens. In this review, we summarize some of the broad insights into S. aureus biology gained from the analysis of genomes and discuss future directions and opportunities in this dynamic field of research.

  18. Antimicrobial (Drug) Resistance: Methicillin-Resistant Staphylococcus aureus (MRSA)

    Science.gov (United States)

    ... Marketing Share this: Main Content Area Methicillin-Resistant Staphylococcus aureus (MRSA) During the past four decades, methicillin-resistant Staphylococcus aureus , or MRSA, has evolved from a controllable ...

  19. Evolution of methicillin-resistant Staphylococcus aureus towards increasing resistance

    DEFF Research Database (Denmark)

    Strommenger, Birgit; Bartels, Mette Damkjær; Kurt, Kevin;

    2014-01-01

    To elucidate the evolutionary history of Staphylococcus aureus clonal complex (CC) 8, which encompasses several globally distributed epidemic lineages, including hospital-associated methicillin-resistant S. aureus (MRSA) and the highly prevalent community-associated MRSA clone USA300....

  20. Propionibacterium acnes biofilm - A sanctuary for Staphylococcus aureus?

    Science.gov (United States)

    Tyner, Harmony; Patel, Robin

    2016-08-01

    The purpose of this study was to measure the effect of combined culture of Propionibacterium acnes and Staphylococcus aureus on biofilm formation under different oxygen concentrations. We measured planktonic growth and biofilm formation of P. acnes and S. aureus alone and together under aerobic and anaerobic conditions. Both P. acnes and S. aureus grew under anaerobic conditions. When grown under anaerobic conditions, P. acnes with or without S. aureus formed a denser biomass biofilm than did S. aureus alone. Viable S. aureus was recovered from a16-day old combined P. acnes and S. aureus biofilm, but not a monomicrobial S. aureus biofilm.

  1. Prevalence of infective endocarditis in patients with Staphylococcus aureus bacteraemia

    DEFF Research Database (Denmark)

    Rasmussen, Rasmus V; Høst, Ulla; Arpi, Magnus;

    2011-01-01

    Aims Staphylococcus aureus infective endocarditis (IE) is a critical medical condition associated with a high morbidity and mortality. In the present study, we prospectively evaluated the importance of screening with echocardiography in an unselected S. aureus bacteraemia (SAB) population. Methods...

  2. Comparative Efficacy of Ceftaroline with Linezolid against Staphylococcus Aureus and Methicillin Resistant Staphylococcus Aureus

    International Nuclear Information System (INIS)

    Objective:To compare the in vitro antimicrobial efficacy of ceftaroline with linezolid against Staphylococcus aureus and methicillin resistant Staphylococcus aureus. Study Design: Quasi-experimental study. Place and Duration of Study: Microbiology Department, Army Medical College, Rawalpindi, from January to December 2013. Methodology: Clinical samples from respiratory tract, blood, pus and various catheter tips routinely received in the Department of Microbiology, Army Medical College, Rawalpindi were innoculated on blood and MacConkey agar. Staphylococcus aureus was identified by colony morphology, Gram reaction, catalase test and coagulase test. Methicillin resistant Staphylococcus aureus detection was done by modified Kirby Bauer disc diffusion method using cefoxitin disc (30g) and the isolates were considered methicillin resistant if the zone of inhibition around cefoxitin disc was /sup 2/ 21 mm. Bacterial suspensions of 56 Staphylococcus aureus isolates and 50 MRSA isolates were prepared, which were standardized equal to 0.5 McFarland's turbidity standard and inoculated on Mueller-Hinton agar plates followed by application of ceftaroline and linezolid disc (Oxoid, UK), according to manufacturer's instructions. The plates were then incubated at 37 Degree C aerobically for 18 - 24 hours. Diameters of inhibition zone were measured and interpretated as per Clinical and Laboratory Standards Institute (CLSI) guidelines. Results: Out of 106 isolates all of the 56 Staphylococcus aureus (100%) were sensitive to ceftaroline and linezolid. However, out of 50 methicillin resistant Staphylococcus aureus, 48 (96%) were sensitive to ceftaroline whereas, 49 (98%) were sensitive to linezolid. Conclusion: Ceftaroline is equally effective as linezolid against Staphylococcus aureus and methicillin resistant Staphylococcus aureus. (author)

  3. Mastite com lesões sistêmicas por Staphylococus aureus subesp. aureus em coelhos Mastitis with systemic lesions due to Staphylococus aureus subesp. aureus in rabbits

    Directory of Open Access Journals (Sweden)

    Sandra Davi Traverso

    2003-04-01

    Full Text Available Em uma criação composta por 1800 coelhos, 33% das matrizes apresentaram mastite e lesões cutâneas crostosas e purulentas. Estes animais apresentavam-se entre 10 a- 12 meses de idade e em segunda parição. Quinze coelhos afetados foram sacrificados e necropsiados. Na necropsia, além das lesões cutâneas haviam microabscessos em diversos órgãos. Das amostras coletadas isolou-se Staphylococcus aureus subesp. aureus. S. aureus subesp. aureus também foi isolado de "swab" nasal coletado do tratador encarregado de fazer o diagnóstico de gestação nas coelhas. Histologicamente, havia formação de múltiplos abscessos, presença de bactérias gram positivas em vasos sangüíneos e linfáticos, além de êmbolos bacterianos nos tecidos. Nas mamas, observou-se tecido glandular normal associado a abscessos multifocais delimitados.At a commercial rabbitry which was composed of 1800 New Zealand White rabbits, 30% of the does had presented mastitis and purulent cutaneal lesions. The age of the animals ranged from 10 to 12 months and were at the second parturition. At necropsy, microabscesses were observed in several organs. Bacteriological samples collected from affected animals resulted Staphylococcus aureus subesp. aureus.. Additionally, the same agent has been isolated from a nasal swab collected from the person responsible for the pregnancy diagnosis. Histologically, there were multiple abscesses, gram positive bacteria within blood and lymphatic vessels, and bacterial emboli scattered in the tissues. In the mammas, normal glandular tissue associated with multifocal abscesses were observed.

  4. Meticillin-resistant Staphylococcus aureus (MRSA)

    DEFF Research Database (Denmark)

    Stefani, Stefania; Chung, Doo Ryeon; Lindsay, Jodi A;

    2012-01-01

    This article reviews recent findings on the global epidemiology of healthcare-acquired/associated (HA), community-acquired/associated (CA) and livestock-associated (LA) meticillin-resistant Staphylococcus aureus (MRSA) and aims to reach a consensus regarding the harmonisation of typing methods...... health. Continuous efforts to understand the changing epidemiology of S. aureus infection in humans and animals are therefore necessary, not only for appropriate antimicrobial treatment and effective infection control but also to monitor the evolution of the species. The group made several consensus...

  5. Guaranteed performance in reaching mode of sliding mode controlled systems

    Indian Academy of Sciences (India)

    G K Singh; K E Holé

    2004-02-01

    Conventionally, the parameters of a sliding mode controller (SMC) are selected so as to reduce the time spent in the reaching mode. Although, an upper bound on the time to reach (reaching time) the sliding surface is easily derived, performance guarantee in the state/error space needs more consideration. This paper addresses the design of constant plus proportional rate reaching law-based SMC for second-order nonlinear systems. It is shown that this controller imposes a bounding second-order error-dynamics, and thus guarantees robust performance during the reaching phase. The choice of the controller parameters based on the time to reach a desirable level of output tracking error (OTE), rather than on the reaching time is proposed. Using the Lyapunov theory, it is shown that parameter selections, based on the reaching time criterion, may need substantially larger time to achieve the OTE. Simulation results are presented for a nonlinear spring-massdamper system. It is seen that parameter selections based on the proposed OTE criterion, result in substantially quicker tracking, while using similar levels of control effort.

  6. Effective bandwidth guaranteed routing schemes for MPLS traffic engineering

    Science.gov (United States)

    Wang, Bin; Jain, Nidhi

    2001-07-01

    In this work, we present online algorithms for dynamic routing bandwidth guaranteed label switched paths (LSPs) where LSP set-up requests (in terms of a pair of ingress and egress routers as well as its bandwidth requirement) arrive one by one and there is no a priori knowledge regarding future LSP set-up requests. In addition, we consider rerouting of LSPs in this work. Rerouting of LSPs has not been well studied in previous work on LSP routing. The need of LSP rerouting arises in a number of ways: occurrence of faults (link and/or node failures), re-optimization of existing LSPs' routes to accommodate traffic fluctuation, requests with higher priorities, and so on. We formulate the bandwidth guaranteed LSP routing with rerouting capability as a multi-commodity flow problem. The solution to this problem is used as the benchmark for comparing other computationally less costly algorithms studied in this paper. Furthermore, to more efficiently utilize the network resources, we propose online routing algorithms which route bandwidth demands over multiple paths at the ingress router to satisfy the customer requests while providing better service survivability. Traffic splitting and distribution over the multiple paths are carefully handled using table-based hashing schemes while the order of packets within a flow is preserved. Preliminary simulations are conducted to show the performance of different design choices and the effectiveness of the rerouting and multi-path routing algorithms in terms of LSP set-up request rejection probability and bandwidth blocking probability.

  7. DEPOSIT GUARANTEE SCHEMES JOIN FINANCIAL SAFETY-NET

    Directory of Open Access Journals (Sweden)

    Eugen Dijmarescu

    2011-03-01

    Full Text Available Deposit Guarantee Schemes (DGS become more visible under the current conditions of the world financial markets. If those have played in the past a rather discreet role for social stability through the function of pay box following a bank failure, the recent crisis put them in a new light, once the guarantee ceiling for eligible deposits grew in order to make for the accumulation of wealth worldwide and the increased risk incurred by the leverage of banking operations. Consequently, the resources accumulated by the DGS from the member banks and their proper management make possible their involvement in pre-emptive actions aimed to avoid bankruptcies via special administration and purchase of assets and assumption of liabilities. These new attributes give DGS a role in the mechanism of financial safety-net, along the supervisors of the market. Hence, an increased preoccupation for applied corporate governance has developed and Core Principles for Effective Deposit Insurance Systems have been adopted by the Basel Committee on Banking Supervision together with International Association of Deposit Insurers. Concurrently, the EU Commission has put forward a process for reviewing the Directives 94/39 and 09/14 EC, in order to make the European DGS more prepared to deal with above mentioned issues and bestow increased confidence upon depositors.

  8. 9 CFR 113.115 - Staphylococcus Aureus Bacterin-Toxoid.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Staphylococcus Aureus Bacterin-Toxoid... REQUIREMENTS Inactivated Bacterial Products § 113.115 Staphylococcus Aureus Bacterin-Toxoid. Staphylococcus Aureus Bacterin-Toxoid shall be prepared from toxoided broth cultures of selected toxogenic strains...

  9. A pig model of acute Staphylococcus aureus induced pyemia

    DEFF Research Database (Denmark)

    Nielsen, O. L.; Iburg, T.; Aalbæk, B.;

    2009-01-01

    Background: Sepsis caused by Staphylococcus aureus constitutes an important cause of morbidity and mortality in humans, and the incidence of this disease-entity is increasing. In this paper we describe the initial microbial dynamics and lesions in pigs experimentally infected with S. aureus....... aureus isolated from man and an extension of the timeframe aiming at inducing sepsis, severe sepsis and septic shock....

  10. Multidrug-Resistant Staphylococcus aureus in US Meat and Poultry

    OpenAIRE

    Waters, Andrew E.; Contente-Cuomo, Tania; Buchhagen, Jordan; Liu, Cindy M.; Watson, Lindsey; Pearce, Kimberly; Foster, Jeffrey T.; Bowers, Jolene; Driebe, Elizabeth M; Engelthaler, David M.; Keim, Paul S; Lance B Price

    2011-01-01

    We characterized the prevalence, antibiotic susceptibility profiles, and genotypes of Staphylococcus aureus among US meat and poultry samples (n = 136). S. aureus contaminated 47% of samples, and multidrug resistance was common among isolates (52%). S. aureus genotypes and resistance profiles differed significantly among sample types, suggesting food animal–specific contamination.

  11. Robust guaranteed cost observer design for linear uncertain jump systems with state delays

    Institute of Scientific and Technical Information of China (English)

    FU Yan-ming; ZHANG Bo; DUAN Guang-ren

    2008-01-01

    This paper deals with the robust guaranteed cost observer with guaranteed cost performance for a class of linear uncertain jump systems with state delay. The transition of the jumping parameters in systems is governed by a finite-state Markov process. Based on the stability theory in stochastic differential equations, a sufficient condition on the existence of the proposed robust guaranteed cost observer is derived. Robust guaran-teed cost observers are designed in terms of a set of linear coupled matrix inequalities. A convex optimization problem with LMI constraints is formulated to design the suboptimal guaranteed cost observers.

  12. Staphylococcus aureus Entrance into the Dairy Chain: Tracking S. aureus from Dairy Cow to Cheese

    Science.gov (United States)

    Kümmel, Judith; Stessl, Beatrix; Gonano, Monika; Walcher, Georg; Bereuter, Othmar; Fricker, Martina; Grunert, Tom; Wagner, Martin; Ehling-Schulz, Monika

    2016-01-01

    Staphylococcus aureus is one of the most important contagious mastitis pathogens in dairy cattle. Due to its zoonotic potential, control of S. aureus is not only of great economic importance in the dairy industry but also a significant public health concern. The aim of this study was to decipher the potential of bovine udder associated S. aureus as reservoir for S. aureus contamination in dairy production and processing. From 18 farms, delivering their milk to an alpine dairy plant for the production of smeared semi-hard and hard cheese. one thousand hundred seventy six one thousand hundred seventy six quarter milk (QM) samples of all cows in lactation (n = 294) and representative samples form bulk tank milk (BTM) of all farms were surveyed for coagulase positive (CPS) and coagulase negative Staphylococci (CNS). Furthermore, samples from different steps of the cheese manufacturing process were tested for CPS and CNS. As revealed by chemometric-assisted FTIR spectroscopy and molecular subtyping (spa typing and multi locus sequence typing), dairy cattle represent indeed an important, yet underreported, entrance point of S. aureus into the dairy chain. Our data clearly show that certain S. aureus subtypes are present in primary production as well as in the cheese processing at the dairy plant. However, although a considerable diversity of S. aureus subtypes was observed in QM and BTM at the farms, only certain S. aureus subtypes were able to enter and persist in the cheese manufacturing at the dairy plant and could be isolated from cheese until day 14 of ripening. Farm strains belonging to the FTIR cluster B1 and B3, which show genetic characteristics (t2953, ST8, enterotoxin profile: sea/sed/sej) of the recently described S. aureus genotype B, most successfully contaminated the cheese production at the dairy plant. Thus, our study fosters the hypothesis that genotype B S. aureus represent a specific challenge in control of S. aureus in the dairy chain that requires

  13. Common R-plasmids in Staphylococcus aureus and Staphylococcus epidermidis during a nosocomial Staphylococcus aureus outbreak.

    OpenAIRE

    Cohen, M. L.; Wong, E. S.; Falkow, S

    1982-01-01

    During a 7-month period in 1978 to 1979, 31 patients and personnel at a Kentucky hospital were colonized or infected with a Staphylococcus aureus strain resistant to clindamycin, erythromycin, gentamicin, methicillin, penicillin, and tetracycline. S. epidermidis with similar antibiotic resistance patterns had been isolated in this hospital in the year before the S. aureus outbreak. A 32-megadalton R-plasmid, pUW3626, mediating resistance to penicillin and gentamicin, was present in these isol...

  14. Staphylococcus aureus resistente a la meticilina (SARM)

    Centers for Disease Control (CDC) Podcasts

    2007-10-22

    Datos importantes sobre las infecciones por SARM en Estados Unidos, en las escuelas y los entornos médicos. (Title: Methicillin-resistant Staphylococcus aureus (MRSA)Created: 10/2007).  Created: 10/22/2007 by National Center for Preparedness, Detection, and Control of Infectious Diseases.   Date Released: 11/9/2007.

  15. Methicillin-resistant Staphylococcus aureus transmission

    DEFF Research Database (Denmark)

    Andersen, Leif Percival; Nielsen, Xiaohui

    2015-01-01

    INTRODUCTION: Even though methicillin-resistant Staphylococcus aureus (MRSA) is a common cause of nosocomial infections, it may often be difficult to evaluate the exact route of transmission. METHODS: In this study, we describe four cases of nosocomial transmission of MRSA in a hospital with a low...

  16. Staphylococcus aureus vaccines: Deviating from the carol.

    Science.gov (United States)

    Missiakas, Dominique; Schneewind, Olaf

    2016-08-22

    Staphylococcus aureus, a commensal of the human nasopharynx and skin, also causes invasive disease, most frequently skin and soft tissue infections. Invasive disease caused by drug-resistant strains, designated MRSA (methicillin-resistant S. aureus), is associated with failure of antibiotic therapy and elevated mortality. Here we review polysaccharide-conjugate and subunit vaccines that were designed to prevent S. aureus infection in patients at risk of bacteremia or surgical wound infection but failed to reach their clinical endpoints. We also discuss vaccines with ongoing trials for combinations of polysaccharide-conjugates and subunits. S. aureus colonization and invasive disease are not associated with the development of protective immune responses, which is attributable to a large spectrum of immune evasion factors. Two evasive strategies, assembly of protective fibrin shields via coagulases and protein A-mediated B cell superantigen activity, are discussed as possible vaccine targets. Although correlates for protective immunity are not yet known, opsonophagocytic killing of staphylococci by phagocytic cells offers opportunities to establish such criteria. PMID:27526714

  17. Carriage of Staphylococcus aureus in the elderly

    NARCIS (Netherlands)

    R. M. Parnaby; G. O'Dwyer; H. A. Monsey; M. S. Shafi

    1996-01-01

    textabstractThe point prevalence and incidence of Staphylococcus aureus (methicillin-sensitive and -resistant) carriage by inpatients on acute elderly care wards was estimated. The relationship to body site and to previous admissions to hospital or other institutions was determined. Fifty-five patie

  18. Increasing resistance of Staphylococcus aureus to ciprofloxacin.

    OpenAIRE

    Daum, T E; Schaberg, D R; Terpenning, M S; Sottile, W S; Kauffman, C A

    1990-01-01

    We demonstrated the marked emergence of resistance to ciprofloxacin among Staphylococcus arueus strains isolated at the Ann Arbor Veterans Administration Medical Center. All S. aureus isolates tested from 1984 to 1985 were susceptible, whereas 55.1% of methicillin-resistant and 2.5% of methicillin-susceptible strains from 1989 had high-level resistance to ciprofloxacin.

  19. Stateless and Delivery Guaranteed Geometric Routing on Virtual Coordinate System

    CERN Document Server

    Liu, Ke

    2008-01-01

    Stateless geographic routing provides relatively good performance at a fixed overhead, which is typically much lower than conventional routing protocols such as AODV. However, the performance of geographic routing is impacted by physical voids, and localization errors. Accordingly, virtual coordinate systems (VCS) were proposed as an alternative approach that is resilient to localization errors and that naturally routes around physical voids. However, VCS also faces virtual anomalies, causing their performance to trail geographic routing. In existing VCS routing protocols, there is a lack of an effective stateless and delivery guaranteed complementary routing algorithm that can be used to traverse voids. Most proposed solutions use variants of flooding or blind searching when a void is encountered. In this paper, we propose a spanning-path virtual coordinate system which can be used as a complete routing algorithm or as the complementary algorithm to greedy forwarding that is invoked when voids are encountere...

  20. Efficient Dynamic Capacity Management Approach for Guarantee SLAs in Clouds

    Directory of Open Access Journals (Sweden)

    Mahfoudh Alasaly

    2016-08-01

    Full Text Available Recently, Cloud computing has created new dimensions for computing. The process of providing service – enabled connection is considered to be the most important function of clouds. This paper which we have carefully thought about, takes into consideration dynamic capacity planning for cloud systems. The objective is to dynamically accommodate computing capacity in order to meet Service Level Agreements (SLAs and, in addition, to minimize the total costs incurred in the cloud services. In this paper, we propose an improvement of a capacity planning scheme to make sure that perfect performance targets of SLAs are always met. The scheme employs threshold-based techniques to guarantee that the SLAs are met and the run-up costs are created to operate on cloud environments and thus has to deal with these environments specific obstacles.

  1. AUTOMATION OF PLC PROGRAMMING WHEN IMPLEMENTING ALGORITHMS OF GUARANTEEING CONTROL

    Directory of Open Access Journals (Sweden)

    M. V. Levinskyi

    2015-05-01

    Full Text Available During developing programs for programmable logic controllers (PLCs the concept of model-oriented design is increasingly used. In particular, usage of Simulink PLC Coder is giving the opportunity to get SCL program codefrom Simulink model which contains certain dynamic elements. Then, for example, this SCL code can be transformed to functional blocks of the Simatic S7-300 (VIPA 300 PLC. This significantly reduces the timerequired to develop code in the language of SCL and reduces requirements for specialists’ qualification when developing control systems. In this article we provide an example of PLC programming automation whenimplementing algorithms of guaranteeing control (AGC. For certain types of technological processes it is typical to contain monotonically increasing function of the effectiveness with fixed one-way restriction in regulations. Forexample, in the grinders, presses, extruders the load current of the drive is stabilized using the change of feed. Energy efficiency of these plants will increase with increasing of the set point (SP to the controller of the drive loadcurrent stabilization loop. However, an increase in SP increases the probability of triggering appropriate protection, for example, as a result of random changes in the properties of raw materials. Therefore, to avoid this accident, thepower of driving motors is often unreasonably overrated. And in this case they are used with currents equal to the half of rated.Systems of guaranteeing control (SGC are used to solve the contradiction between the need to improvethe efficiency and increasing probability of an accident.

  2. Relationship between Vancomycin-Resistant Staphylococcus aureus, Vancomycin-Intermediate S. aureus, High Vancomycin MIC, and Outcome in Serious S. aureus Infections

    OpenAIRE

    Holmes, Natasha E.; Johnson, Paul D. R.; Howden, Benjamin P.

    2012-01-01

    Vancomycin has been used successfully for over 50 years for the treatment of Staphylococcus aureus infections, particularly those involving methicillin-resistant S. aureus. It has proven remarkably reliable, but its efficacy is now being questioned with the emergence of strains of S. aureus that display heteroresistance, intermediate resistance, and, occasionally, complete vancomycin resistance. More recently, an association has been established between poor outcome and infections with strain...

  3. Staphylococcus aureus Shifts toward Commensalism in Response to Corynebacterium Species.

    Science.gov (United States)

    Ramsey, Matthew M; Freire, Marcelo O; Gabrilska, Rebecca A; Rumbaugh, Kendra P; Lemon, Katherine P

    2016-01-01

    Staphylococcus aureus-human interactions result in a continuum of outcomes from commensalism to pathogenesis. S. aureus is a clinically important pathogen that asymptomatically colonizes ~25% of humans as a member of the nostril and skin microbiota, where it resides with other bacteria including commensal Corynebacterium species. Commensal Corynebacterium spp. are also positively correlated with S. aureus in chronic polymicrobial diabetic foot infections, distinct from acute monomicrobial S. aureus infections. Recent work by our lab and others indicates that microbe-microbe interactions between S. aureus and human skin/nasal commensals, including Corynebacterium species, affect S. aureus behavior and fitness. Thus, we hypothesized that S. aureus interactions with Corynebacterium spp. diminish S. aureus virulence. We tested this by assaying for changes in S. aureus gene expression during in vitro mono- versus coculture with Corynebacterium striatum, a common skin and nasal commensal. We observed a broad shift in S. aureus gene transcription during in vitro growth with C. striatum, including increased transcription of genes known to exhibit increased expression during human nasal colonization and decreased transcription of virulence genes. S. aureus uses several regulatory pathways to transition between commensal and pathogenic states. One of these, the quorum signal accessory gene regulator (agr) system, was strongly inhibited in response to Corynebacterium spp. Phenotypically, S. aureus exposed to C. striatum exhibited increased adhesion to epithelial cells, reflecting a commensal state, and decreased hemolysin activity, reflecting an attenuation of virulence. Consistent with this, S. aureus displayed diminished fitness in experimental in vivo coinfection with C. striatum when compared to monoinfection. These data support a model in which S. aureus shifts from virulence toward a commensal state when exposed to commensal Corynebacterium species.

  4. Staphylococcus aureus Shifts toward Commensalism in Response to Corynebacterium Species.

    Science.gov (United States)

    Ramsey, Matthew M; Freire, Marcelo O; Gabrilska, Rebecca A; Rumbaugh, Kendra P; Lemon, Katherine P

    2016-01-01

    Staphylococcus aureus-human interactions result in a continuum of outcomes from commensalism to pathogenesis. S. aureus is a clinically important pathogen that asymptomatically colonizes ~25% of humans as a member of the nostril and skin microbiota, where it resides with other bacteria including commensal Corynebacterium species. Commensal Corynebacterium spp. are also positively correlated with S. aureus in chronic polymicrobial diabetic foot infections, distinct from acute monomicrobial S. aureus infections. Recent work by our lab and others indicates that microbe-microbe interactions between S. aureus and human skin/nasal commensals, including Corynebacterium species, affect S. aureus behavior and fitness. Thus, we hypothesized that S. aureus interactions with Corynebacterium spp. diminish S. aureus virulence. We tested this by assaying for changes in S. aureus gene expression during in vitro mono- versus coculture with Corynebacterium striatum, a common skin and nasal commensal. We observed a broad shift in S. aureus gene transcription during in vitro growth with C. striatum, including increased transcription of genes known to exhibit increased expression during human nasal colonization and decreased transcription of virulence genes. S. aureus uses several regulatory pathways to transition between commensal and pathogenic states. One of these, the quorum signal accessory gene regulator (agr) system, was strongly inhibited in response to Corynebacterium spp. Phenotypically, S. aureus exposed to C. striatum exhibited increased adhesion to epithelial cells, reflecting a commensal state, and decreased hemolysin activity, reflecting an attenuation of virulence. Consistent with this, S. aureus displayed diminished fitness in experimental in vivo coinfection with C. striatum when compared to monoinfection. These data support a model in which S. aureus shifts from virulence toward a commensal state when exposed to commensal Corynebacterium species. PMID:27582729

  5. Strategic design of cost savings guarantee in energy performance contracting under uncertainty

    International Nuclear Information System (INIS)

    Highlights: • A methodology is proposed to assist Energy Service Companies to maintain competitiveness in winning bids. • Uncertainties within the energy cost savings are modeled stochastically using the Monte-Carlo simulation. • A strategic energy savings guarantee design curve is derived, where all points return as appropriate guarantees. • A campus case is presented to demonstrate the applicability for finding appropriate guaranteed savings value. - Abstract: Among the key barriers to profit in Energy Performance Contracting (EPC) are uncertainties about attaining the realized energy cost savings and potential disputes over the guaranteed cost savings. In this paper, a methodology has been proposed to assist the Energy Service Company (ESCO): (1) to evaluate the risk threshold if the guarantee has already been made, and (2) to determine the guarantee design, if the guarantee has not been made yet, that not only promises the ESCO’s profitability from EPC but also maintains its competitiveness to win the bid. Uncertainties within the energy cost savings are modeled stochastically using Monte-Carlo simulation, taking both the energy price fluctuation and the facility performance variability into account. Based on that, a strategic energy savings guarantee design curve is derived, that all the points on it would return as appropriate guarantees. Finally, a campus case is presented to demonstrate the applicability for finding the appropriate guaranteed savings value. This method is also worth popularizing in similar performance-based projects

  6. Optimal guaranteed cost control for fuzzy descriptor systems with time-varying delay

    Institute of Scientific and Technical Information of China (English)

    Tian Weihua; Zhang Huaguang

    2008-01-01

    Based on the delay-independent rule, the problem of optimal guaranteed cost control for a class of Takagi-Sugeno (T-S) fuzzy descriptor systems with time-varying delay is studied. A linear quadratic cost function is considered as the performance index of the closed-loop system. Sufficient conditions for the existence of guaranteed cost controllers via state feedback are given in terms of linear matrix inequalities (LMIs), and the design of an optimal guaranteed cost controller can be reduced to a convex optimization problem. It is shown that the designed controller not only guarantees the asymptotic stability of the closed-loop fuzzy descriptor delay system, but also provides an optimized upper bound of the guaranteed cost. At last, a numerical example is given to illustrate the effectiveness of the proposed method and the perfect performance of the optimal guaranteed cost controller.

  7. Staphylococcus aureus shifts towards commensalism in response to Corynebacterium species

    Directory of Open Access Journals (Sweden)

    Matthew M Ramsey

    2016-08-01

    Full Text Available Staphylococcus aureus–human interactions result in a continuum of outcomes from commensalism to pathogenesis. S. aureus is a clinically important pathogen that asymptomatically colonizes ~25% of humans as a member of the nostril and skin microbiota, where it resides with other bacteria including commensal Corynebacterium species. Commensal Corynebacterium spp. are also positively correlated with S. aureus in chronic polymicrobial diabetic foot infections, distinct from acute monomicrobial S. aureus infections. Recent work by our lab and others indicates that microbe-microbe interactions between S. aureus and human skin/nasal commensals, including Corynebacterium species, affect S. aureus behavior and fitness. Thus, we hypothesized that S. aureus interactions with Corynebacterium spp. diminish S. aureus virulence. We tested this by assaying for changes in S. aureus gene expression during in vitro mono- versus coculture with Corynebacterium striatum, a common skin and nasal commensal. We observed a broad shift in S. aureus gene transcription during in vitro growth with C. striatum, including increased transcription of genes known to exhibit increased expression during human nasal colonization and decreased transcription of virulence genes. S. aureus uses several regulatory pathways to transition between commensal and pathogenic states. One of these, the quorum signal accessory gene regulator (agr system, was strongly inhibited in response to Corynebacterium spp. Phenotypically, S. aureus exposed to C. striatum exhibited increased adhesion to epithelial cells, reflecting a commensal state, and decreased hemolysin activity, reflecting an attenuation of virulence. Consistent with this, S. aureus displayed diminished fitness in experimental in vivo coinfection with C. striatum when compared to monoinfection. These data support a model in which S. aureus shifts from virulence towards a commensal state when exposed to commensal Corynebacterium species.

  8. Guaranteed cost control of uncertain system with input time-delay

    Institute of Scientific and Technical Information of China (English)

    胡乃平; 唐功友

    2003-01-01

    For the uncertain continuous-time systems with input time-delay that widely exist in the production processes, we can get the existent conditions for the guaranteed cost control of these systems by using the Lyapunov stability theory, linear matrix inequalities theory and quadratic cost criterion. We can achieve the guaranteed cost control of this system by solving a matrix inequality. A state feed back guaranteed cost control law can be constructed by solving certain parameter-dependent Riccati matrix equation.

  9. Methicillin-resistant Staphylococcus aureus: the superbug.

    Science.gov (United States)

    Ippolito, Giuseppe; Leone, Sebastiano; Lauria, Francesco N; Nicastri, Emanuele; Wenzel, Richard P

    2010-10-01

    Over the last decade, methicillin-resistant Staphylococcus aureus (MRSA) strains have emerged as serious pathogens in the nosocomial and community setting. Hospitalization costs associated with MRSA infections are substantially greater than those associated with methicillin-sensitive S. aureus (MSSA) infections, and MRSA has wider economic effects that involve indirect costs to the patient and to society. In addition, there is some evidence suggesting that MRSA infections increase morbidity and the risk of mortality. Glycopeptides are the backbone antibiotics for the treatment of MRSA infections. However, several recent reports have highlighted the limitations of vancomycin, and its role in the management of serious infections is now being reconsidered. Several new antimicrobials demonstrate in vitro activity against MRSA and other Gram-positive bacteria. Data from large surveys indicate that linezolid, daptomycin, and tigecycline are almost universally active against MRSA. This review will briefly discuss the epidemiology, costs, outcome, and therapeutic options for the management of MRSA infections. PMID:20851011

  10. Curcumin Reverse Methicillin Resistance in Staphylococcus aureus

    OpenAIRE

    Su-Hyun Mun; Sung-Bae Kim; Ryong Kong; Jang-Gi Choi; Youn-Chul Kim; Dong-Won Shin; Ok-Hwa Kang; Dong-Yeul Kwon

    2014-01-01

    Curcumin, a natural polyphenolic flavonoid extracted from the rhizome of Curcuma longa L., was shown to possess superior potency to resensitize methicillin-resistant Staphylococcus aureus (MRSA) to antibiotics. Previous studies have shown the synergistic activity of curcumin with β-lactam and quinolone antibiotics. Further, to understand the anti-MRSA mechanism of curcumin, we investigated the potentiated effect of curcumin by its interaction in diverse conditions. The mechanism of anti-MRSA ...

  11. Mixed H2/H∞ Optimal Guaranteed Cost Control of Uncertain Linear Systems

    Institute of Scientific and Technical Information of China (English)

    GuodingChen; MayingYang; LiYu

    2004-01-01

    The mixed H2/H∞ guaranteed cost control problem via state feedback control laws is considered in this paper for linear systems with norm-bounded parameter uncertainty. Based on the linear matrix inequality (LMI) approach, sufficient conditions are derived for the existence of guaranteed cost controllers whihc guarantee not only a prespecified H∞ disturbance attenuation level on one controlled output for all admissible parameter uncertainties, but also the worst-case H2 performance index on the other controlled output to be no more than a specified bound. Furthermore, a convex optimization problem is formulated to design an optimal H2/H∞ guaranteed cost controller.

  12. A Scheduling Discipline for Latency and Bandwidth Guarantees in Asynchronous Network-on-Chip

    DEFF Research Database (Denmark)

    Bjerregaard, Tobias; Sparsø, Jens

    2005-01-01

    Guaranteed services (GS) are important in that they provide predictability in the complex dynamics of shared communication structures. This paper discusses the implementation of GS in asynchronous Network-on-Chip. We present a novel scheduling discipline called Asynchronous Latency Guarantee (ALG......) scheduling, which provides latency and bandwidth guarantees in accessing a shared media, e.g. a physical link shared between a number of virtual channels. ALG overcomes the drawbacks of existing scheduling disciplines, in particular the coupling between latency and bandwidth guarantees. A 0.12 &956;m CMOS...

  13. COCOA PRODUCTION - AGRICULTURAL CREDIT GUARANTEE SCHEME FUND NEXUS IN NIGERIA: A COINTEGRATION APPROACH

    Directory of Open Access Journals (Sweden)

    O. Oyakhilomen

    2012-09-01

    Full Text Available This study was carried out to examine the relationship between cocoa production in Nigeria and agricultural credit guarantee scheme fund using time series data on cocoa production in Nigeria, value of loans guaranteed and number of loans guaranteed spanning over the period of 1981 to 2011. The Johansen cointegration test was employed in this study and the result indicated that there was no cointegrating relationship between cocoa production in Nigeria and Agricultural credit guarantee scheme fund over the period under study. This could be attributed to the guaranteeing of few number as well as limited value of credit to the farmers by agricultural credit guarantee scheme fund and the high incidence of loan diversion by the cocoa farmers who had access to the loans guaranteed by agricultural credit guarantee scheme fund. It is recommended that the number as well as the value of credit guaranteed to cocoa farmers should be significantly increased so as to enable the farmers expand their production and thereby, reposition the cocoa to assume a critical role as a major non-oil foreign exchange earner in the Agricultural transformation plan of Nigeria.

  14. On routing algorithms with end-to-end delay guarantees

    Energy Technology Data Exchange (ETDEWEB)

    Rao, N.S.V.; Batsell, S.G.

    1998-11-01

    The authors consider the transmission of a message of size r from a source to a destination with guarantees on the end-to-end delay over a computer network with n nodes and m links. There are three sources of delays: (a) propagation delays along the links, (b) delays due to bandwidth availability on the links, and (c) queuing delays at the intermediate nodes. First, the authors consider that delays on various links and nodes are given as functions of the message size. If the delay in (b) is a non-increasing function of the bandwidth, they propose O(m{sup 2} + mn log n) time algorithm to compute a path with the minimum end-to-end delay for any given message size r. They then consider that the queuing delay in (c) is a random variable correlated with the message size according to an unknown distribution. At each node, the measurements of queuing delays and message sizes are available. They propose two algorithms to compute paths whose delays are close to optimal delays with a high probability, irrespective of the distribution of the delays, and based entirely on the measurements of sufficient size.

  15. Ensuring QOS Guarantees in a Hybrid OCS/OBS Network

    CERN Document Server

    S, Sunish Kumar O

    2012-01-01

    The bursting aggregation assembly in edge nodes is one of the key technologies in OBS (Optical Burst Switching) network, which has a direct impact on flow characteristics and packet loss rate. An optical burst assembly technique supporting QoS is presented through this paper, which can automatically adjust the threshold along with the increasing and decreasing volume of business, reduce the operational burst, and generate corresponding BDP (Burst Data Packet) and BCP (Burst Control Packet). In addition to the burst aggregation technique a packet recovery technique by restoration method is also described. The data packet loss due to the physical optical link failure is not currently included in the QoS descriptions. This link failure is also a severe problem which reduces the data throughput of the transmitter node. A mechanism for data recovery from this link failure is vital for guaranteeing the QoS demanded by each user. So this paper will also discusses a specific protocol for reducing the packet loss by u...

  16. Nuclear power: status, outlook, guarantees of sustainable development

    International Nuclear Information System (INIS)

    Full text: The principal advantages of nuclear power - almost unlimited fuel resources, its high energy capacity, ecological compatibility with a possibility of high wastes concentration - determine the large-scale nuclear power development. The signs of large-scale power - a large rate (dozens of percent) in electricity production, diverse areas (electricity, heat supply, technologies, transport) and media of application (land, ocean, space), extension of number of user countries, diversified power systems (centralized, autonomous), obligatory reproduction and reuse of produced fuel - create various requirements to nuclear power installations of the future. Economic efficiency and competitiveness, safety (of reactors and fuel cycle with waste), proper characteristics of nuclear fuel reproduction, guarantees of nuclear arm's non-proliferation and, particularly, public acceptance are the conditions of such nuclear power development. The up-to-date situation is the following: the 441 nuclear power-generation units with total installed power of 377.36 GW el. (in 31 countries) supply by 1/6 part of the world electric-power consumptions. The 32 units are in stage of the construction. To the present mid-century the level of the nuclear power production, as supposed, must be increased 4-5 times at the following scenario of a regional distribution of nuclear electric powers, GW: USA, Europe and developed countries of Eastern Asia - 1000, FSU-countries - 100 and developing countries - 400

  17. Guaranteed Blind Sparse Spikes Deconvolution via Lifting and Convex Optimization

    Science.gov (United States)

    Chi, Yuejie

    2016-06-01

    Neural recordings, returns from radars and sonars, images in astronomy and single-molecule microscopy can be modeled as a linear superposition of a small number of scaled and delayed copies of a band-limited or diffraction-limited point spread function, which is either determined by the nature or designed by the users; in other words, we observe the convolution between a point spread function and a sparse spike signal with unknown amplitudes and delays. While it is of great interest to accurately resolve the spike signal from as few samples as possible, however, when the point spread function is not known a priori, this problem is terribly ill-posed. This paper proposes a convex optimization framework to simultaneously estimate the point spread function as well as the spike signal, by mildly constraining the point spread function to lie in a known low-dimensional subspace. By applying the lifting trick, we obtain an underdetermined linear system of an ensemble of signals with joint spectral sparsity, to which atomic norm minimization is applied. Under mild randomness assumptions of the low-dimensional subspace as well as a separation condition of the spike signal, we prove the proposed algorithm, dubbed as AtomicLift, is guaranteed to recover the spike signal up to a scaling factor as soon as the number of samples is large enough. The extension of AtomicLift to handle noisy measurements is also discussed. Numerical examples are provided to validate the effectiveness of the proposed approaches.

  18. Functional modules of sigma factor regulons guarantee adaptability and evolvability

    Science.gov (United States)

    Binder, Sebastian C.; Eckweiler, Denitsa; Schulz, Sebastian; Bielecka, Agata; Nicolai, Tanja; Franke, Raimo; Häussler, Susanne; Meyer-Hermann, Michael

    2016-02-01

    The focus of modern molecular biology turns from assigning functions to individual genes towards understanding the expression and regulation of complex sets of molecules. Here, we provide evidence that alternative sigma factor regulons in the pathogen Pseudomonas aeruginosa largely represent insulated functional modules which provide a critical level of biological organization involved in general adaptation and survival processes. Analysis of the operational state of the sigma factor network revealed that transcription factors functionally couple the sigma factor regulons and significantly modulate the transcription levels in the face of challenging environments. The threshold quality of newly evolved transcription factors was reached faster and more robustly in in silico testing when the structural organization of sigma factor networks was taken into account. These results indicate that the modular structures of alternative sigma factor regulons provide P. aeruginosa with a robust framework to function adequately in its environment and at the same time facilitate evolutionary change. Our data support the view that widespread modularity guarantees robustness of biological networks and is a key driver of evolvability.

  19. Relieving the Wireless Infrastructure: When Opportunistic Networks Meet Guaranteed Delays

    CERN Document Server

    Whitbeck, John; Leguay, Jérémie; Conan, Vania; de Amorim, Marcelo Dias

    2010-01-01

    Major wireless operators are nowadays facing network capacity issues in striving to meet the growing demands of mobile users. However, 3G-enabled devices increasingly benefit from ad hoc radio connectivity (e.g., Wi-Fi). In this context of hybrid connectivity, we propose Push-and-track, a content dissemination framework that harnesses ad hoc communication opportunities to minimize the load on the wireless infrastructure while guaranteeing tight delivery delays. It achieves this through a control loop that collects user-sent acknowledgements to determine if new copies need to be reinjected into the network through the 3G interface. Push-and-Track includes multiple strategies to determine how many copies of the content should be injected and to whom. The short delay-tolerance of common content, such as news or road traffic updates, make them suitable for such a system. Based on a realistic large-scale vehicular dataset from the city of Bologna composed of more than 10,000 vehicles, we demonstrate that Push-and-...

  20. Toxin-Antitoxin Systems of Staphylococcus aureus.

    Science.gov (United States)

    Schuster, Christopher F; Bertram, Ralph

    2016-05-05

    Toxin-antitoxin (TA) systems are small genetic elements found in the majority of prokaryotes. They encode toxin proteins that interfere with vital cellular functions and are counteracted by antitoxins. Dependent on the chemical nature of the antitoxins (protein or RNA) and how they control the activity of the toxin, TA systems are currently divided into six different types. Genes comprising the TA types I, II and III have been identified in Staphylococcus aureus. MazF, the toxin of the mazEF locus is a sequence-specific RNase that cleaves a number of transcripts, including those encoding pathogenicity factors. Two yefM-yoeB paralogs represent two independent, but auto-regulated TA systems that give rise to ribosome-dependent RNases. In addition, omega/epsilon/zeta constitutes a tripartite TA system that supposedly plays a role in the stabilization of resistance factors. The SprA1/SprA1AS and SprF1/SprG1 systems are post-transcriptionally regulated by RNA antitoxins and encode small membrane damaging proteins. TA systems controlled by interaction between toxin protein and antitoxin RNA have been identified in S. aureus in silico, but not yet experimentally proven. A closer inspection of possible links between TA systems and S. aureus pathophysiology will reveal, if these genetic loci may represent druggable targets. The modification of a staphylococcal TA toxin to a cyclopeptide antibiotic highlights the potential of TA systems as rather untapped sources of drug discovery.

  1. Aspartate inhibits Staphylococcus aureus biofilm formation.

    Science.gov (United States)

    Yang, Hang; Wang, Mengyue; Yu, Junping; Wei, Hongping

    2015-04-01

    Biofilm formation renders Staphylococcus aureus highly resistant to conventional antibiotics and host defenses. Four D-amino acids (D-Leu, D-Met, D-Trp and D-Tyr) have been reported to be able to inhibit biofilm formation and disassemble established S. aureus biofilms. We report here for the first time that both D- and L-isoforms of aspartate (Asp) inhibited S. aureus biofilm formation on tissue culture plates. Similar biofilm inhibition effects were also observed against other staphylococcal strains, including S. saprophyticus, S. equorum, S. chromogenes and S. haemolyticus. It was found that Asp at high concentrations (>10 mM) inhibited the growth of planktonic N315 cells, but at subinhibitory concentrations decreased the cellular metabolic activity without influencing cell growth. The decreased cellular metabolic activity might be the reason for the production of less protein and DNA in the matrix of the biofilms formed in the presence of Asp. However, varied inhibition efficacies of Asp were observed for biofilms formed by clinical staphylococcal isolates. There might be mechanisms other than decreasing the metabolic activity, e.g. the biofilm phenotypes, affecting biofilm formation in the presence of Asp.

  2. Aspartate inhibits Staphylococcus aureus biofilm formation.

    Science.gov (United States)

    Yang, Hang; Wang, Mengyue; Yu, Junping; Wei, Hongping

    2015-04-01

    Biofilm formation renders Staphylococcus aureus highly resistant to conventional antibiotics and host defenses. Four D-amino acids (D-Leu, D-Met, D-Trp and D-Tyr) have been reported to be able to inhibit biofilm formation and disassemble established S. aureus biofilms. We report here for the first time that both D- and L-isoforms of aspartate (Asp) inhibited S. aureus biofilm formation on tissue culture plates. Similar biofilm inhibition effects were also observed against other staphylococcal strains, including S. saprophyticus, S. equorum, S. chromogenes and S. haemolyticus. It was found that Asp at high concentrations (>10 mM) inhibited the growth of planktonic N315 cells, but at subinhibitory concentrations decreased the cellular metabolic activity without influencing cell growth. The decreased cellular metabolic activity might be the reason for the production of less protein and DNA in the matrix of the biofilms formed in the presence of Asp. However, varied inhibition efficacies of Asp were observed for biofilms formed by clinical staphylococcal isolates. There might be mechanisms other than decreasing the metabolic activity, e.g. the biofilm phenotypes, affecting biofilm formation in the presence of Asp. PMID:25687923

  3. Whole-Genome Sequence of Staphylococcus aureus Strain LCT-SA112

    OpenAIRE

    Wang, Junfeng; Liu, Yanhong; Wan, Daiwei; Fang, Xiangqun; Li, Tianzhi; Guo, Yinghua; Chang, De; Su, Longxiang; Wang, Yajuan; Zhao, Jiao; Liu, Changting

    2012-01-01

    Staphylococcus aureus is a facultative anaerobic Gram-positive coccal bacterium. S. aureus is the most common species of Staphylococcus to cause staphylococcal infections, which are very common in clinical medicine. Here we report the genome sequence of S. aureus strain LCT-SA112, which was isolated from S. aureus subsp. aureus CGMCC 1.230.

  4. SAMMD: Staphylococcus aureus Microarray Meta-Database

    Directory of Open Access Journals (Sweden)

    Elasri Mohamed O

    2007-10-01

    Full Text Available Abstract Background Staphylococcus aureus is an important human pathogen, causing a wide variety of diseases ranging from superficial skin infections to severe life threatening infections. S. aureus is one of the leading causes of nosocomial infections. Its ability to resist multiple antibiotics poses a growing public health problem. In order to understand the mechanism of pathogenesis of S. aureus, several global expression profiles have been developed. These transcriptional profiles included regulatory mutants of S. aureus and growth of wild type under different growth conditions. The abundance of these profiles has generated a large amount of data without a uniform annotation system to comprehensively examine them. We report the development of the Staphylococcus aureus Microarray meta-database (SAMMD which includes data from all the published transcriptional profiles. SAMMD is a web-accessible database that helps users to perform a variety of analysis against and within the existing transcriptional profiles. Description SAMMD is a relational database that uses MySQL as the back end and PHP/JavaScript/DHTML as the front end. The database is normalized and consists of five tables, which holds information about gene annotations, regulated gene lists, experimental details, references, and other details. SAMMD data is collected from the peer-reviewed published articles. Data extraction and conversion was done using perl scripts while data entry was done through phpMyAdmin tool. The database is accessible via a web interface that contains several features such as a simple search by ORF ID, gene name, gene product name, advanced search using gene lists, comparing among datasets, browsing, downloading, statistics, and help. The database is licensed under General Public License (GPL. Conclusion SAMMD is hosted and available at http://www.bioinformatics.org/sammd/. Currently there are over 9500 entries for regulated genes, from 67 microarray

  5. Pricing of the Cross-Currency Interest Rate Guarantee Embedded in Financial Contracts in a LIBOR Market Model

    OpenAIRE

    Tsung-Yu Hsieh; Chi-Hsun Chou; Son-Nan Chen

    2015-01-01

    We derive the pricing formula for the financial contracts, such as guaranteed investment contracts (GICs), life insurance contracts, pension plans, and others, with the guaranteed minimum rate of return set relative to a LIBOR interest rate. Further, we analyze the guaranteed contracts in which the asset that provides the underlying return for the contract and the guaranteed interest rate are denominated in different currencies, which is a common practice. The guaranteed contracts with the ab...

  6. Antibody responses in patients with invasive Staphylococcus aureus infections

    OpenAIRE

    Jacobsson, G; Colque-Navarro, P.; Gustafsson, E.; Andersson, R.; Möllby, R

    2010-01-01

    Abstract Correlation between antibody response and clinical outcome in Staphylococcus aureus bacteremia has yielded conflicting results. Immunization schedules have failed in clinical trials. Is the humoral response toward S. aureus of protective nature? A prospective study was performed in patients with invasive S. aureus (ISA) infections during the period 2003?2005. The antibody levels were determined at the beginning and at the end of treatment and one month later (n?=?96, n?=?7...

  7. Mapping the Distribution of Invasive Staphylococcus aureus across Europe

    OpenAIRE

    Grundmann, Hajo; Aanensen, David M.; van den Wijngaard, Cees C.; Brian G Spratt; Harmsen, Dag; Friedrich, Alexander W.; ,

    2010-01-01

    Editors' Summary Background The bacterium Staphylococcus aureus lives on the skin and in the nose of about a third of healthy people. Although S. aureus usually coexists peacefully with its human carriers, it is also an important disease-causing organism or pathogen. If it enters the body through a cut or during a surgical procedure, S. aureus can cause minor infections such as pimples and boils or more serious, life-threatening infections such as blood poisoning and pneumonia. Minor S. aureu...

  8. Staphylococcus aureus atsparumas antibiotikams ir fagotipų paplitimas

    OpenAIRE

    Kareivienė, Violeta; Pavilonis, Alvydas; Sinkutė, Gintarė; Liegiūtė, Sigutė; Gailienė, Greta

    2006-01-01

    Objective. The aim of this study was to identify the phage groups of Staphylococcus aureus strains, their prevalence, and resistance of different phage groups to antibiotics. Materials and methods. A total of 294 Staphylococcus aureus strains in Kaunas hospitals were obtained; they were phage typed and their resistance to antibiotics was determined. We used the method of routine dilution to test 17 antibiotics against the isolates. Susceptibility of Staphylococcus aureus to studied antibio...

  9. Threat of drug resistant Staphylococcus aureus to health in Nepal

    OpenAIRE

    Ansari, Shamshul; Nepal, Hari Prasad; Gautam, Rajendra; Rayamajhi, Nabin; Shrestha, Sony; Upadhyay, Goma; Acharya, Anju; Chapagain, Moti Lal

    2014-01-01

    Background Staphylococcus aureus is the most commonly isolated organism from the different clinical samples in hospital. The emergence and dissemination of methicillin resistant Staphylococcus aureus (MRSA) and growing resistance to non-beta-lactam antibiotics is making treatment of infections due to this organism increasingly difficult. Methods This study was conducted to determine the frequency of Staphylococcus aureus isolated from different clinical samples, rates of MRSA and full antibio...

  10. Daya Hambat Ekstrak Aloe Vera terhadap pertumbuhan Staphylococcus Aureus

    OpenAIRE

    Rahmat, drg.Sp,Pros

    2011-01-01

    Dari hasil penelitian , maka dapat disimpulkan bahwa ekstrak Aloe Vera dapat menghambat pertumbuhan bakteri Stafhylococcus aureus, dan kadar hambat minimal ekstrak Aloe Vera adalah pada konsentrasi 25%.

  11. μ distortions or running: A guaranteed discovery from CMB spectrometry

    Science.gov (United States)

    Cabass, Giovanni; Melchiorri, Alessandro; Pajer, Enrico

    2016-04-01

    We discuss the implications of a PIXIE-like experiment, which would measure μ -type spectral distortions of the cosmic microwave background (CMB) at a level of σμ=(1 /n )×10-8 , with n ≥1 representing an improved sensitivity (e.g. n =10 corresponds to PRISM). Using Planck data and considering the six-parameter Λ CDM model, we compute the posterior for μ8≡μ ×108 and find μ8=1.5 7-0.13+0.11 (68% C.L.). This becomes μ8=1.2 8-0.52+0.30 (68% C.L.) when the running αs of the spectral index is included. We point out that a sensitivity of about 3 ×PIXIE implies a guaranteed discovery: μ distortion is detected or αs≥0 is excluded (both at 95% C.L. or higher). This threshold sensitivity sets a clear benchmark for CMB spectrometry. For a combined analysis of PIXIE and current Planck data, we discuss the improvement on measurements of the tilt ns and the running αs and the dependence on the choice of the pivot. A fiducial running of αs=-0.01 (close to the Planck best fit) leads to a detection of negative running at 2 σ for 5 ×PIXIE . A fiducial running of αs=-0.02 , still compatible with Planck, requires 3 ×PIXIE to rule out αs=0 (at 95% C.L.). We propose a convenient and compact visualization of the improving constraints on the tilt, running and tensor-to-scalar ratio.

  12. 7 CFR 1717.655 - Exclusion of certain investments, loans, and guarantees.

    Science.gov (United States)

    2010-01-01

    ... waste disposal systems; (C) Telecommunication and other electronic communication systems; and (D... AND GUARANTEED ELECTRIC LOANS Investments, Loans, and Guarantees by Electric Borrowers § 1717.655..., and CoBank, ACB; (3) Patronage capital allocated from an electric power supply cooperative of...

  13. 7 CFR 1940.588 - Business and Industry Guaranteed and Direct Loans.

    Science.gov (United States)

    2010-01-01

    ... Allocation of Loan and Grant Program Funds § 1940.588 Business and Industry Guaranteed and Direct Loans. (a... 7 Agriculture 13 2010-01-01 2009-01-01 true Business and Industry Guaranteed and Direct Loans... HOUSING SERVICE, RURAL BUSINESS-COOPERATIVE SERVICE, RURAL UTILITIES SERVICE, AND FARM SERVICE...

  14. 20 CFR 229.10 - What the social security overall minimum guarantee is.

    Science.gov (United States)

    2010-04-01

    ... payable before the employee attains retirement age. An overall minimum may also be payable before age 62... guarantee is. 229.10 Section 229.10 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD RETIREMENT ACT SOCIAL SECURITY OVERALL MINIMUM GUARANTEE Social Security Overall Minimum...

  15. 78 FR 64175 - Hashemite Kingdom of Jordan Loan Guarantees Issued Under the Further Continuing Appropriations...

    Science.gov (United States)

    2013-10-28

    .... Person means any legal person, including any individual, corporation, partnership, joint venture... applicable to loan guarantees to be issued for the benefit of the Hashemite Kingdom of Jordan pursuant to the... conditions applicable to loan guarantees issued for the benefit of the Borrower, pursuant to Title III of...

  16. VISA/VRSA (Vancomycin-Intermediate/Resistant Staphylococcus aureus) in Healthcare Settings

    Science.gov (United States)

    ... to vancomycin and other antimicrobial agents. What is Staphylococcus aureus? Staphylococcus aureus is a bacterium commonly found on the ... control personnel. Investigation and Control of Vancomycin-Resistant Staphylococcus aureus (VRSA) [PDF - 300 KB] - This document is ...

  17. Robust guaranteed cost filtering for uncertain time-delay systems with Markovian jumping parameters

    Institute of Scientific and Technical Information of China (English)

    Fu Yanming; Zhang Ying; Duan Guangren; Chai Qingxuan

    2005-01-01

    The robust guaranteed cost filtering problem for a class of linear uncertain stochastic systems with time delays is investigated. The system under study involves time delays, jumping parameters and Brownian motions. The transition of the jumping parameters in systems is governed by a finite-state Markov process. The objective is to design linear memoryless filters such that for all uncertainties, the resulting augmented system is robust stochastically stable independent of delays and satisfies the proposed guaranteed cost performance. Based on stability theory in stochastic differential equations, a sufficient condition on the existence of robust guaranteed cost filters is derived. Robust guaranteed cost filters are designed in terms of linear matrix inequalities. A convex optimization problem with LMI constraints is formulated to design the suboptimal guaranteed cost filters.

  18. Staphylococcus aureus: resistance pattern and risk factors

    Directory of Open Access Journals (Sweden)

    Mohammad Naghavi-Behzad

    2015-03-01

    Full Text Available Introduction: Methicillin resistant Staphylococcus aureus (MRSA has emerged as a nosocomial pathogen of major worldwide importance and is an increasingly frequent cause of community-acquired infections. In this study, different risk factors and MRSA resistance pattern were investigated. Methods: In a 24 months period, all of the patients who were confined to bed in the surgery ward were included in the study. Then they were assessed to find out as if they had MRSA infection when hospitalized and once when they were discharged. Almost 48 h after admission, when patients were discharged, social and medical histories were acquired. Acquired samples were examined. Results: During the present study of 475 patients, 108 patients (22.8% had S. aureus. About frequency of antibiotic resistance among collected S. aureus colonies, erythromycin resistance, was the most frequent antibiotic resistance, also resistance to vancomycin was 0.4% that was the least. Only hospitalization duration had statistically significant correlation with antibiotic resistance, also resistance to erythromycin had statistically significant relation with history of surgery and alcohol consumption. Of all 34 MRSA species, 22 (64.7% samples were resistant to erythromycin, 17 (50.0% resistant to cefoxitin, 5 (14.7% resistant to mupirocin, 1 (2.9% resistant to vancomycin and 1 (2.9% resistant to linezolid. Conclusion: The results of the current study show that among hospitalized patients, there is resistance against methicillin. Since based on results of the study there is resistance against oxacillin and erythromycin in most cases, administering appropriate antibiotics have an important role in minimizing the resistance burden among bacterial species.

  19. Resistencia antimicrobiana de cepas de Staphylococcus aureus, Costa Rica Antimicrobial resistance of Staphylococcus aureus, Costa Rica

    Directory of Open Access Journals (Sweden)

    Víctor Hugo Alvarado

    2011-12-01

    Full Text Available Objetivo: Determinar y comparar los perfiles de resistencia de cepas de S. aureus aisladas de quesos, producidos en la Zona Sur de Costa Rica y de un centro hospitalario de la misma región. Materiales y Métodos: Se analizaron 35 muestras de queso fresco, adquiridas durante los meses de setiembre y octubre del 2010 en la zona de San Vito de Coto Brus. A cada muestra se le realizaron recuentos de coliformes totales, coliformes fecales y Staphylococcus aureus. Adicionalmente se analizó presencia/ausencia de Listeria monocytogenes en 25 gramos del producto. A las cepas identificadas como S. aureus se les realizó la prueba de sensibilidad a los antibióticos mediante el sistema automatizado Vitek y la interpretación de los datos se realizó siguiendo las pautas del Clinical and Laboratory Standards Institute antimicrobial susceptibility testing 2011. Adicional a esto se recolectaron datos acerca de la sensibilidad de las cepas de S. aureus aisladas e identificadas en el Hospital de San Vito de Coto Brus en el mismo período. Resultados: El promedio obtenido para el recuento de coliformes totales fue de 9,7 X 10(6 UFC/g, para coliformes fecales de 6,7 X 10(5 y para S. aureus de 2,8 X 10(5 UFC/g, obteniéndose un 83 % de muestras positivas por esta bacteria. En cuanto a la resistencia antimicrobiana, se obtuvieron porcentajes de resistencia mayores en las cepas de origen clínico. Se encontró también que 23 de las cepas (96% provenientes de muestras clínicas, presentaban resistencia a más de un antibiótico, mientras que siete de las obtenidas a partir de queso (27% presentaban esta característica. Con respecto a los betalactamicos (ampicilina, oxacilina y penicilina se observó la existencia de una diferencia estadísticamente significativa (pObjective: determined and compared the resistance patters of S. aureus strains isolated from cheese produced in the southern zone of Costa Rica and from clinical samples isolated at the hospital center

  20. Methicillin-resistant Staphylococcus aureus laryngitis.

    Science.gov (United States)

    Liakos, Tracey; Kaye, Keith; Rubin, Adam D

    2010-09-01

    Infections due to methicillin-resistant Staphylococcus aureus (MRSA) have become more prevalent, in part because of the emergence and spread of community-acquired MRSA. This trend is particularly concerning because of the significant rates of morbidity and mortality associated with MRSA infections, and because MRSA strains are often resistant to many classes of antibiotics. Reports of infections of the head and neck, including wound infections, cellulitis, sinusitis, otitis media, and otitis externa, are well documented. However, to our knowledge, there have been no reports of bacterial laryngitis due to MRSA. We report the first published case of bacterial laryngitis caused by MRSA.

  1. Staphylococcus aureus bacteriuria as a prognosticator for outcome of Staphylococcus aureus bacteremia: a case-control study

    Directory of Open Access Journals (Sweden)

    Weinstein Robert A

    2010-07-01

    Full Text Available Abstract Background When Staphylococcus aureus is isolated in urine, it is thought to usually represent hematogenous spread. Because such spread might have special clinical significance, we evaluated predictors and outcomes of S. aureus bacteriuria among patients with S. aureus bacteremia. Methods A case-control study was performed at John H. Stroger Jr. Hospital of Cook County among adult inpatients during January 2002-December 2006. Cases and controls had positive and negative urine cultures, respectively, for S. aureus, within 72 hours of positive blood culture for S. aureus. Controls were sampled randomly in a 1:4 ratio. Univariate and multivariable logistic regression analyses were done. Results Overall, 59% of patients were African-American, 12% died, 56% of infections had community-onset infections, and 58% were infected with methicillin-susceptible S. aureus (MSSA. Among 61 cases and 247 controls, predictors of S. aureus bacteriuria on multivariate analysis were urological surgery (OR = 3.4, p = 0.06 and genitourinary infection (OR = 9.2, p = 0.002. Among patients who died, there were significantly more patients with bacteriuria than among patients who survived (39% vs. 17%; p = 0.002. In multiple Cox regression analysis, death risks in bacteremic patients were bacteriuria (hazard ratio 2.9, CI 1.4-5.9, p = 0.004, bladder catheter use (2.0, 1.0-4.0, p = 0.06, and Charlson score (1.1, 1.1-1.3, p = 0.02. Neither length of stay nor methicillin-resistant Staphylococcus aureus (MRSA infection was a predictor of S. aureus bacteriuria or death. Conclusions Among patients with S. aureus bacteremia, those with S. aureus bacteriuria had 3-fold higher mortality than those without bacteriuria, even after adjustment for comorbidities. Bacteriuria may identify patients with more severe bacteremia, who are at risk of worse outcomes.

  2. Intracellular Staphylococcus aureus : live-in and let die

    NARCIS (Netherlands)

    Fraunholz, Martin; Sinha, Bhanu

    2012-01-01

    Staphylococcus aureus uses a plethora of virulence factors to accommodate a diversity of niches in its human host. Aside from the classical manifestations of S. aureus-induced diseases, the pathogen also invades and survives within mammalian host cells. The survival strategies of the pathogen are as

  3. Duplex Identification of Staphylococcus aureus by Aptamer and Gold Nanoparticles.

    Science.gov (United States)

    Chang, Tianjun; Wang, Libo; Zhao, Kexu; Ge, Yu; He, Meng; Li, Gang

    2016-06-01

    Staphylococcus aureus is the top common pathogen causing infections and food poisoning. Identification of S. aureus is crucial for the disease diagnosis and regulation of food hygiene. Herein, we report an aptamer-AuNPs based method for duplex identification of S. aureus. Using AuNPs as an indicator, SA23, an aptamer against S. aureus, can well identify its target from Escherichia coli, Listeria monocytogenes and Pseudomonas aeruginosa. Furthermore, we find citrate-coated AuNPs can strongly bind to S. aureus, but not bind to Salmonella enterica and Proteus mirabilis, which leads to different color changes in salt solution. This colorimetric response is capable of distinguishing S. aureus from S. enteritidis and P. mirabilis. Thus, using the aptasensor and AuNPs together, S. aureus can be accurately identified from the common pathogens. This duplex identification system is a promising platform for simple visual identification of S. aureus. Additionally, in the aptasensing process, bacteria are incubated with aptamers and then be removed before the aptamers adding to AuNPs, which may avoid the interactions between bacteria and AuNPs. This strategy can be potentially applied in principle to detect other cells by AuNPs-based aptasensors. PMID:27427591

  4. Staphylococcus aureus and the ecology of the nasal microbiome

    DEFF Research Database (Denmark)

    Liu, Cindy M; Price, Lance B; Hungate, Bruce A;

    2015-01-01

    The human microbiome can play a key role in host susceptibility to pathogens, including in the nasal cavity, a site favored by Staphylococcus aureus. However, what determines our resident nasal microbiota-the host or the environment-and can interactions among nasal bacteria determine S. aureus...

  5. Duplex Identification of Staphylococcus aureus by Aptamer and Gold Nanoparticles.

    Science.gov (United States)

    Chang, Tianjun; Wang, Libo; Zhao, Kexu; Ge, Yu; He, Meng; Li, Gang

    2016-06-01

    Staphylococcus aureus is the top common pathogen causing infections and food poisoning. Identification of S. aureus is crucial for the disease diagnosis and regulation of food hygiene. Herein, we report an aptamer-AuNPs based method for duplex identification of S. aureus. Using AuNPs as an indicator, SA23, an aptamer against S. aureus, can well identify its target from Escherichia coli, Listeria monocytogenes and Pseudomonas aeruginosa. Furthermore, we find citrate-coated AuNPs can strongly bind to S. aureus, but not bind to Salmonella enterica and Proteus mirabilis, which leads to different color changes in salt solution. This colorimetric response is capable of distinguishing S. aureus from S. enteritidis and P. mirabilis. Thus, using the aptasensor and AuNPs together, S. aureus can be accurately identified from the common pathogens. This duplex identification system is a promising platform for simple visual identification of S. aureus. Additionally, in the aptasensing process, bacteria are incubated with aptamers and then be removed before the aptamers adding to AuNPs, which may avoid the interactions between bacteria and AuNPs. This strategy can be potentially applied in principle to detect other cells by AuNPs-based aptasensors.

  6. Effect of Mupirocin on Nasal Carriage of Staphylococcus Aureus

    NARCIS (Netherlands)

    M. Bulanda; M. Gruszka; B. Heczko

    1989-01-01

    textabstractMupirocin eliminates nasal carriage of staphylococcal aureus among medical and surgical personnel for periode varying from several weeks upto one year. In persons recolonized after therapy densites of S. aureus population in nares were much lower than in the same persons before therapy.

  7. Susceptibility of methicillin-resistant Staphylococcus aureus to lysostaphin.

    OpenAIRE

    Huber, M M; Huber, T. W.

    1989-01-01

    One hundred and eleven isolates of methicillin-resistant Staphylococcus aureus recovered from patients at the Olin E. Teague Veterans Center from March 1983 to April 1987 were as susceptible to lysis by lysostaphin as methicillin-susceptible S. aureus controls were.

  8. Mechanism of resistance to some cephalosporins in Staphylococcus aureus.

    OpenAIRE

    Kono, M; Sasatsu, M; O'Hara, K; Shiomi, Y.; HAYASAKA, T.

    1983-01-01

    The mechanism of resistance to some cephalosporins in Staphylococcus aureus strains was investigated with high-pressure liquid chromatography and nuclear magnetic resonance spectrometry. Drug inactivation by penicillinase was found to be the main mechanism of resistance to cefazolin, cephaloridine, and cephalothin in S. aureus.

  9. Activity of and Resistance to Moxifloxacin in Staphylococcus aureus

    OpenAIRE

    Ince, Dilek; Zhang, Xiamei; Hooper, David C.

    2003-01-01

    Moxifloxacin has enhanced potency against Staphylococcus aureus, lower propensity to select for resistant mutants, and higher bactericidal activity against highly resistant strains than ciprofloxacin. Despite similar activity against purified S. aureus topoisomerase IV and DNA gyrase, it selects for topoisomerase IV mutants, making topoisomerase IV the preferred target in vivo.

  10. Changing Trends in Resistance Pattern of Methicillin Resistant Staphylococcus aureus

    OpenAIRE

    Kali, Arunava; Stephen, Selvaraj; Umadevi, Sivaraman; Kumar, Shailesh; Joseph, Noyal Mariya; Srirangaraj, Sreenivasan

    2013-01-01

    Background: Methicillin resistance in Staphylococcus aureus is associated with multidrug resistance, an aggressive course, increased mortality and morbidity in both community and health care facilities. Monitoring of newly emerging and prevalent Methicillin Resistant Staphylococcus aureus (MRSA) strains for their resistance patterns to conventional as well as novel drugs, are essential for infection control.

  11. Pneumonia and new methicillin-resistant Staphylococcus aureus clone.

    NARCIS (Netherlands)

    Garnier, Fabien; Tristan, Anne; François, Bruno; Etienne, Jerome; Delage-Corre, Manuella; Martin, Christian; Liassine, Nadia; Wannet, Wim; Denis, François; Ploy, Marie-Cécile

    2006-01-01

    Necrotizing pneumonia caused by Staphylococcus aureus strains carrying the Panton-Valentin leukocidin gene is a newly described disease entity. We report a new fatal case of necrotizing pneumonia. An S. aureus strain with an agr1 allele and of a new sequence type 377 was recovered, representing a ne

  12. The Present Situation of Chattel Guarantee System and Its Improvements - Taking the Angl of American Uniform Commercial Code (UCC)

    Institute of Scientific and Technical Information of China (English)

    仇晓光; 刘聪

    2011-01-01

    Although there are appropriate stipulations for the chattel guarantees in the law of China,this guarantee system could not adapt to the request of economy's development because of the following reasons:the scope of chattel guarantees is narrow according to relevant stipulations; the transaction cost of the chattel is high; mortgage registration of chattel is in a mess; lack of efficiently executive guarantee system,etc.To counter the problems existing in the present Chinese chattel guarantee system,in this article,the author proposed some corresponding suggestions and countermeasures,hoping to perfect the chattel guarantee system in China.

  13. Pricing of the Cross-Currency Interest Rate Guarantee Embedded in Financial Contracts in a LIBOR Market Model

    Directory of Open Access Journals (Sweden)

    Tsung-Yu Hsieh

    2015-12-01

    Full Text Available We derive the pricing formula for the financial contracts, such as guaranteed investment contracts (GICs, life insurance contracts, pension plans, and others, with the guaranteed minimum rate of return set relative to a LIBOR interest rate. Further, we analyze the guaranteed contracts in which the asset that provides the underlying return for the contract and the guaranteed interest rate are denominated in different currencies, which is a common practice. The guaranteed contracts with the above characteristics are called “cross-currency interest rate guaranteed contracts” (CIRGCs. Valuation of such contracts has not been investigated in the previous literature.

  14. A porcine model of haematogenous brain infectionwith staphylococcus aureus

    DEFF Research Database (Denmark)

    Astrup, Lærke Boye; Agerholm, Jørgen Steen; Nielsen, Ole Lerberg;

    2012-01-01

    A PORCINE MODEL OF HAEMATOGENOUS BRAIN INFECTION WITH STAPHYLOCOCCUS AUREUS Astrup Lærke1, Agerholm Jørgen1, Nielsen Ole1, Jensen Henrik1, Leifsson Páll1, Iburg Tine2. 1: Faculty of Health and Medical Sciences, University of Copenhagen, Denmark boye@life.ku.dk 2: National Veterinary Institute......, Uppsala, Sweden Introduction Staphylococcus aureus (S.aureus) is a common cause of sepsis and brain abscesses in man and a frequent cause of porcine pyaemia. Here we present a porcine model of haematogenous S. aureus-induced brain infection. Materials and Methods Four pigs had two intravenous catheters...... inserted surgically, one in a. carotis communis and one in v. jugularis externa. All pigs received 106 CFU/kg body weight S. aureus through the arterial catheter. Bacteria were either suspended in isotonic saline infused at constant flow for 60 minutes (two pigs) or given as a bolus injection of autologoue...

  15. Characterization of methicillin-resistant Staphylococcus aureus Sequence Type 398

    DEFF Research Database (Denmark)

    Christiansen, Mette Theilgaard

    Staphylococcus aureus is an opportunistic pathogen that colonizes the nares and skin surfaces of several animal species, including man. S. aureus can cause a wide variety of infections ranging from superficial soft tissue and skin infections to severe and deadly systemic infections. Traditionally S....... aureus and methicillin-resistant Staphylococcus aureus (MRSA) have been associated with hospitals, but during the past decades MRSA has emerged in the community and now a new branch of MRSA has been found in association with livestock (LA-MRSA). A specific lineage (multilocus sequence type 398 (ST398...... for LA-MRSA ST398 survival on porcine skin and nasal epithelium ex vivo were identified. These genes could represent targets for de-colonization, which could help prevent further spread and adaption of LA-MRSA ST398. Manuscript III describes the construction of the S. aureus VirulenceFinder database...

  16. Antimicrobial drug resistance ofStaphylococcus aureus in dairy products

    Institute of Scientific and Technical Information of China (English)

    Sasidharan S; Prema B; Yoga Latha L

    2011-01-01

    Objective:To evaluate the prevalence of multidrug resistantStaphylococcus aureus(S. aureus) in dairy products.Methods:Isolation and identification ofS. aureus were performed in3 dairy-based food products. The isolates were tested for their susceptibility to5 different common antimicrobial drugs.Results:Of50 samples examined,5 (10%) were contaminated with S. aureus. Subsequently, the5 isolates were subjected to antimicrobial resistance pattern using five antibiotic discs (methicillin, vancomycin, kanamycin, chloramphenicol and tetracycline). Sample 29 showed resistance to methicillin and vancomycin. Sample18 showed intermediate response to tetracycline. The other samples were susceptible to all the antibiotics tested.Conclusions:The results provide preliminary data on sources of food contamination which may act as vehicles for the transmission of antimicrobial-resistantStaphylococcus.Therefore, it enables us to develop preventive strategies to avoid the emergence of new strains of resistantS. aureus.

  17. The Impact of Guarantees on the Performance of Pension Saving Schemes: Insights from the Literature

    Directory of Open Access Journals (Sweden)

    Alexander Bohnert

    2015-11-01

    Full Text Available Guarantees are often seen as the key characteristics of pension saving products, but securing them can become costly and is of central relevance especially in the course of the current low interest rate environment. In this article, we deal with the question of how costly the typical types of guarantees are, in the sense that they reduce a pension saving scheme’s financial performance over time. In this context, we aim to provide a presentation of insights from selected literature studying the impact of point-to-point guarantees and cliquet-style interest rate guarantees on the performance of pension contracts. The comparative analysis emphasizes that, in most cases, guarantee costs are not negligible with regard to a contract’s financial performance, especially compared to benchmarks, and that customers knowingly opt for such guarantees (or not is, thus, indispensable. To further investigate the willingness-to-pay for guarantees in life insurance is an area for future research, in particular for innovative contract design.

  18. Where does a Staphylococcus aureus vaccine stand?

    Science.gov (United States)

    Fowler, V G; Proctor, R A

    2014-05-01

    In this review, we examine the current status of Staphylococcus aureus vaccine development and the prospects for future vaccines. Examination of the clinical trials to date show that murine models have not predicted success in humans for active or passive immunization. A key factor in the failure to develop a vaccine to prevent S. aureus infections comes from our relatively limited knowledge of human protective immunity. More recent reports on the elements of the human immune response to staphylococci are analysed. In addition, there is some controversy concerning the role of antibodies for protecting humans, and these data are reviewed. From a review of the current state of understanding of staphylococcal immunity, a working model is proposed. Some new work has provided some initial candidate biomarker(s) to predict outcomes of invasive infections and to predict the efficacy of antibiotic therapy in humans. We conclude by looking to the future through the perspective of lessons gleaned from the clinical vaccine trials. PMID:24476315

  19. Efektivitas Ekstrak Daun Jambu Biji Buah Putih Terhadap Pertumbuhan Staphylococcus aureus Dari Abses Dan Staphylococcus aureus (ATCC® 29213™)

    OpenAIRE

    Sinurat, Jojor

    2016-01-01

    Daun jambu biji mengandung senyawa aktif seperti tanin, triterpenoid, flavonoid, saponin yang mempunyai efek antibakteri. Mekanisme tanin sebagai antibakteri dengan mengkerutkan dinding sel dan membran sel, inaktivasi enzim, inaktivasi fungsi materi genetik bakteri. Flavonoid merusak sel bakteri, denaturasi protein, inaktivasi enzim dan menyebabkan lisis. Triterpenoid dan saponin menghambat pertumbuhan Staphylococcus aureus dengan cara merusak struktur membran sel. Staphylococcus aureus adala...

  20. Non-fragile hybrid guaranteed cost control for a class of uncertain switched linear systems

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    This paper focuses on the problem of non-fragile hybrid guaranteed cost control for a class of uncertain switched linear systems. The controller gain to be designed is assumed to have additive gain variations. Based on multiple-Lyapunov function technique, the design of non-fragile hybrid guaranteed cost controllers is derived to make the corresponding closed-loop system asymptotically stable for all admissible uncertainties. Furthermore, a convex optimization approach with LMIs constraints is introduced to select the optimal non-fragile guaranteed cost controllers. Finally, a simulation example illustrates the effectiveness of the proposed approach.

  1. Delay-independent robust guaranteed-cost control for uncertain linear neutral systems

    Institute of Scientific and Technical Information of China (English)

    Li Hongfei; Zhou Jun

    2007-01-01

    This article concerns the delay-independent guaranteed-cost control problem via memoryless state feedback for a class of neutral-type systems with structural uncertainty and a given quadratic cost function. New delay-independent conditions for the existence of the guaranteed-cost controller are presented in the term of LMIs. An algorithm involving optimization is proposed to design a controller achieving an optimal guaranteed-cost, such that, the system can be stabilized for all admissible uncertainties. A numerical example is provided to illustrate the feasibility of the proposed method.

  2. Do not pay for a Danish interest guarantee. The law of the triple blow

    DEFF Research Database (Denmark)

    Guillén, Montserrat; Konicz, Agnieszka Karolina; Perch Nielsen, Jens;

    2013-01-01

    that the price of an interest guarantee is high in pension products that are currently commercialised in the market. We have found that the customer loses up to about 0.75% yearly in the rate of return when an interest guarantee is purchased, compared to the return of an equivalent saving strategy with the same...... risk at the level 95%. This can explain why such arrangements are not widely popular. Our approach can be used to inform clients, who are not experts in modern financial models, the impact of paying for an interest guarantee....

  3. ROBUST STABILITY WITH GUARANTEEING COST FOR DISCRETE TIME-DELAY SYSTEMS WITH NONLINEAR PERTURBATION

    Institute of Scientific and Technical Information of China (English)

    JIA Xinchun; ZHENG Nanning; LIU Yuehu

    2005-01-01

    The problems of robust stability and robust stability with a guaranteeing cost for discrete time-delay systems with nonlinear perturbation are discussed. A sufficient criterion for robust stability is established in an LMI framework and a linear convex optimization problem with LMI constraints for computing maximal perturbation bound is proposed. Meanwhile, a sufficient criterion for robust stability with a guaranteeing cost for such systems is obtained, and an optimal procedure for decreasing the value of guaranteeing cost is put forward. Two examples are used to illustrate the efficiency of the results.

  4. Petrifilm rapid S. aureus Count Plate method for rapid enumeration of Staphylococcus aureus in selected foods: collaborative study.

    Science.gov (United States)

    Silbernagel, K M; Lindberg, K G

    2001-01-01

    A rehydratable dry-film plating method for Staphylococcus aureus in foods, the 3M Petrifilm Rapid S. aureus Count Plate method, was compared with AOAC Official Method 975.55 (Staphylococcus aureus in Foods). Nine foods-instant nonfat dried milk, dry seasoned vegetable coating, frozen hash browns, frozen cooked chicken patty, frozen ground raw pork, shredded cheddar cheese, fresh green beans, pasta filled with beef and cheese, and egg custard-were analyzed for S. aureus by 13 collaborating laboratories. For each food tested, the collaborators received 8 blind test samples consisting of a control sample and 3 levels of inoculated test sample, each in duplicate. The mean log counts for the methods were comparable for pasta filled with beef and cheese; frozen hash browns; cooked chicken patty; egg custard; frozen ground raw pork; and instant nonfat dried milk. The repeatability and reproducibility variances of the Petrifilm Rapid S. aureus Count Plate method were similar to those of the standard method.

  5. The molecular changing mechanism of Ampicillin-Sulbactam resistant Staphylococcus aureus towards Methicillin resistant Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Mieke Hemiawati Satari

    2005-12-01

    Full Text Available The aim of this study was to determine the molecular changing of S.aureus, which is resistant to Ampicillin-Sulbactam and then become resistant to Methicillin as a result of improper dosage. The study was conducted by isolating Ampicillin-Sulbactam resistant and Methicillin Resistant S.aureus (MRSA, afterwards an amplification process was performed by PCR (Polymerase Chain Reaction. to isolate the betalactamase enzyme regulator and PBP 2a genes. The result of this research showed that there were a deletion of few amino acids from the regulator gene, and a suspicion that the DNA sequence had been substituted from PBP 2 gene into PBP 2a (gen mec. This process had formed MRSA.

  6. Supply guarantee initiatives for nuclear fuel materials and services and their compatibility with the market

    International Nuclear Information System (INIS)

    This paper first discusses where and why those ideas for nuclear fuel supply assurance and guarantee mechanisms came out, and attempts to draw a comparison among the proposed schemes and thereby examine possible steps forward. (author)

  7. 7 CFR 1738.30 - Rural broadband access loans and loan guarantees.

    Science.gov (United States)

    2010-01-01

    ... includes commercial banks, trust companies, mortgage banking firms, insurance companies, and any other... be, will neither be paid first nor given any preference or priority over the Guaranteed Loan...

  8. Investigating the effects of issuance of bank debt guarantee on profitability of banking industry

    Directory of Open Access Journals (Sweden)

    Reza Pirayesh

    2014-02-01

    Full Text Available This paper presents an empirical investigation to study the relationship between bank debt guarantee and profitability, increase in banks resources and customer retention. The proposed study has been implemented in one of Iranian banks located in city of Zanjan, Iran over the period of 10 years. The study has used Pearson correlation test to examine three hypotheses of the survey. The results indicate that there were positive and meaningful relationship between bank debt guarantee and profitability (r = 0.863, P-value = 0.001, a positive relationship between bank debt guarantee and bank resources (r = 0.708 with P-value = 0.015 and there was a positive and meaningful relationship between bank debt guarantee and customer retention (r = 0.252 with P-value = 0.001.

  9. 78 FR 76593 - Maximum Loan Amount for Business and Industry Guaranteed Loans in Fiscal Year 2014

    Science.gov (United States)

    2013-12-18

    ... financing assistance to as many projects as possible. In order for an existing B&I guaranteed loan borrower... current for the past 12 months without such status being achieved through debt forgiveness; and (3) not...

  10. 78 FR 691 - Maximum Loan Amount for Business and Industry Guaranteed Loans in Fiscal Year 2013

    Science.gov (United States)

    2013-01-04

    ... financing assistance to as many projects as possible. In order for an existing B&I guaranteed loan borrower... current for the past 12 months without such status being achieved through debt forgiveness; and (3) not...

  11. A Best Practice for Developing Availability Guarantee Language in Photovoltaic (PV) O&M Agreements.

    Energy Technology Data Exchange (ETDEWEB)

    Klise, Geoffrey Taylor [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Balfour, John [High Performance PV, Phoenix, AZ (United States)

    2015-11-01

    This document outlines the foundation for developing language that can be utilized in an Equipment Availability Guarantee, typically included in an O&M services agreement between a PV system or plant owner and an O&M services provider, or operator. Many of the current PV O&M service agreement Availability Guarantees are based on contracts used for traditional power generation, which create challenges for owners and operators due to the variable nature of grid-tied photovoltaic generating technologies. This report documents language used in early PV availability guarantees and presents best practices and equations that can be used to more openly communicate how the reliability of the PV system and plant equipment can be expressed in an availability guarantee. This work will improve the bankability of PV systems by providing greater transparency into the equipment reliability state to all parties involved in an O&M services contract.

  12. Reliable fuzzy control with domain guaranteed cost for fuzzy systems with actuator failures

    Institute of Scientific and Technical Information of China (English)

    JIA Xinchun; ZHENG Nanning

    2004-01-01

    The reliable fuzzy control with guaranteed cost for T-S fuzzy systems with actuator failure is proposed in this paper. The cost function is a quadratic function with failure input. When the initial state of such systems is known, a design method of the reliable fuzzy controller with reliable guaranteed cost is presented, and the formula of the guaranteed cost is established. When the initial state of such systems is unknown but belongs to a known bounded closed domain, a notion of the reliable domain guaranteed cost (RDGC) for such systems is proposed. For two classes of initial state domain, polygon domain and ellipsoid domain, some design methods for reliable fuzzy controllers with the RDGC are provided. The efficiency of our design methods is finally verified by numerical design and simulation on the Rossler chaotic system.

  13. Is biomass always a renewable fuel as guaranteed?

    International Nuclear Information System (INIS)

    alternative electric energy must be energy which is not produced from oil shale. TheLong-Term Development Plan for the Estonian Fuel and Energy Sector includes such misleading terms as peat, wood and renewable energy resources, biofuels and peat. However, there are also quite correct phrases such as peat, wood and other renewable natural resources, as wood and other biofuels are renewable natural resources. As a rule, wood and wood waste, energy cultures, agricultural, woodpulp and paper industry waste, as well as solid animal breeding and household waste, and also peat are classified as biomass in many countries. Biomass seems to be a more convenient term instead of renewable biofuel. In most statements it is with no doubt classified as renewable resource, while biofuels are not because fossil fuels are also biofuels (or fuels of biological origin) and they are renewable only to a very limited extent. Fossil fuels originated in sapropel and peat, oil and natural gas from dispersed organic matter, too. So, their recovery depends on the speed of accumulation of organic carbon in recent sediments. The recovery should not be a question of what somebody believes in or not. It must not be a 'religious' question but a purely scientific and methodological issue. There is only one condition when a fuel (or biomass as one type of biofuel) is renewable: biomass or biofuel may be considered renewable if they are replenished at a rate which is comparable to the rate at which they are consumed in a country or part of it (on an island, for example). The overused amount is a non-renewable biomass or biofuel. So, the replenishment of biomass or biofuel is not guaranteed. Biomass and other biofuels, including fossil ones, are products of the activity of the sun. There are also other, non-fuel types of energy such as water and wind energy, as well as solar and geothermal energy. They are also considered renewable energy resources as is biomass. All these types of energy (except, may be, the

  14. Indole and 7-benzyloxyindole attenuate the virulence of Staphylococcus aureus.

    Science.gov (United States)

    Lee, Jin-Hyung; Cho, Hyun Seob; Kim, Younghoon; Kim, Jung-Ae; Banskota, Suhrid; Cho, Moo Hwan; Lee, Jintae

    2013-05-01

    Human pathogens can readily develop drug resistance due to the long-term use of antibiotics that mostly inhibit bacterial growth. Unlike antibiotics, antivirulence compounds diminish bacterial virulence without affecting cell viability and thus, may not lead to drug resistance. Staphylococcus aureus is a major agent of nosocomial infections and produces diverse virulence factors, such as the yellow carotenoid staphyloxanthin, which promotes resistance to reactive oxygen species (ROS) and the host immune system. To identify novel antivirulence compounds, bacterial signal indole present in animal gut and diverse indole derivatives were investigated with respect to reducing staphyloxanthin production and the hemolytic activity of S. aureus. Treatment with indole or its derivative 7-benzyloxyindole (7BOI) caused S. aureus to become colorless and inhibited its hemolytic ability without affecting bacterial growth. As a result, S. aureus was more easily killed by hydrogen peroxide (H₂O₂) and by human whole blood in the presence of indole or 7BOI. In addition, 7BOI attenuated S. aureus virulence in an in vivo model of nematode Caenorhabditis elegans, which is readily infected and killed by S. aureus. Transcriptional analyses showed that both indole and 7BOI repressed the expressions of several virulence genes such as α-hemolysin gene hla, enterotoxin seb, and the protease genes splA and sspA and modulated the expressions of the important regulatory genes agrA and sarA. These findings show that indole derivatives are potential candidates for use in antivirulence strategies against persistent S. aureus infection. PMID:23318836

  15. The changing epidemiology of Staphylococcus aureus bloodstream infection

    DEFF Research Database (Denmark)

    Galbraith, J.C.; Valiquette, G.; Kennedy, K.J.;

    2013-01-01

    Clin Microbiol Infect ABSTRACT: Although the epidemiology of Staphylococcus aureus bloodstream infection (BSI) has been changing, international comparisons are lacking. We sought to determine the incidence of S. aureus BSI and assess trends over time and by region. Population-based surveillance...... episodes of S. aureus BSI were identified. The overall annual incidence rate for S. aureus BSI was 26.1 per 100 000 population, and those for methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) were 24.2 and 1.9 per 100 000, respectively. Although the overall incidence...... of community-onset MSSA BSI (15.0 per 100 000) was relatively similar across regions, the incidence rates of hospital-onset MSSA (9.2 per 100 000), community-onset MRSA (1.0 per 100 000) and hospital-onset MRSA (0.8 per 100 000) BSI varied substantially. Whereas the overall incidence of S. aureus BSI did...

  16. Comparison of the guaranteed analysis with the measured nutrient composition of commercial pet foods

    OpenAIRE

    Hill, Richard C.; Choate, Christina J; Scott, Karen C.; Molenberghs, Geert

    2009-01-01

    Objective-To compare guaranteed and measured concentrations of nutrients in commercial pet foods. Design-Cross-sectional study. Sample Population-Annual inspection reports of pet food analyses from 5 states. Procedures-Guaranteed and measured concentrations of crude protein (CP), crude fat (CF), crude fiber (CFb), moisture, and ash in pet foods were compared. The concentration difference for each nutrient was compared among types of food, target species, target life stages, manufacturers, and...

  17. Guaranteed cost control for uncertain stochastic fuzzy systems with time delay

    Institute of Scientific and Technical Information of China (English)

    ZHANG Huaguang; WANG Yingchun

    2007-01-01

    This paper studies a delay-dependent guaranteed cost control problem for a class of uncertain nonlinear stochastic systems with time delay represented by the Takagi-Sugeno (T-S) fuzzy model with uncertain parameters. The descriptor system method and Gu's inequality are employed to obtain delay-dependent sufficient conditions such that the closed-loop system is asymptotically stable with a certain guaranteed cost control performance. The effectiveness of the proposed method is shown by a simulation example.

  18. The Implementation of Service Guarantees in China Service and Manufacturing Industries

    OpenAIRE

    Tang, Lan

    2011-01-01

    With the development of economy in the world, people would like to spend more money to enjoy their life. In this way, service industry becomes more popular than manufacturing industry. In addition, although the main business of a company is production, it will provide additional services, such as after-sales services, to increase its products’ selling volume. At the same time, a good service quality should be improved and guaranteed by applying service guarantee. However, China is a developin...

  19. Delay-dependent guaranteed cost control for uncertain systems with both state and input delays

    Institute of Scientific and Technical Information of China (English)

    Xuanfang YANG; Wuhua CHEN; Huajing FANG

    2004-01-01

    This paper considers the guaranteed cost control problem for a class of uncertain linear systems with both state and input delays.By representing the time-delay system in the descriptor system form and using a recent result on bounding of cross products of vectors,we obtain new delay-dependent sufficient conditions for the existence of the guaranteed cost controller in terms of linear matrix inequalities.Two examples are presented which show the effectiveness of our approach.

  20. A Simple Method for Guaranteeing ECG Quality in Real-Time Wavelet Lossy Coding

    OpenAIRE

    Alesanco Álvaro; García José

    2007-01-01

    Guaranteeing ECG signal quality in wavelet lossy compression methods is essential for clinical acceptability of reconstructed signals. In this paper, we present a simple and efficient method for guaranteeing reconstruction quality measured using the new distortion index wavelet weighted PRD (WWPRD), which reflects in a more accurate way the real clinical distortion of the compressed signal. The method is based on the wavelet transform and its subsequent coding using the set partitioning in h...

  1. 7 CFR Appendix I to Subpart E of... - Instructions for Loan Guarantees for Drought and Disaster Relief

    Science.gov (United States)

    2010-01-01

    ... guaranteed is governed by 7 CFR Part 1980 Subpart E Appendix I and the Loan Note Guarantee (Drought and... incontestable except for fraud or misrepresentation of which the Lender has actual knowledge at the time it... instruments (including personal and/or corporate guarantees) will remain with the Lender and in all...

  2. Identification of the ClpX Regulon in Staphylococcus aureus

    DEFF Research Database (Denmark)

    Jelsbak, Lotte; Thomsen, Line Elnif; Ingmer, Hanne;

    Staphyloccous aureus is a major human pathogen capable of causing a wide spectrum of infections ranging from superficial wound infections to life-threatening endocarditis and toxic shock syndrome. Essential for S. aureus virulence is a large number of cell-surface-associated proteins and secreted...... we show here that almost 400 genes (15%) are influenced by the clpX deletion. Furthermore, ClpX not only regulates many virulence factors, but rather serves as a global regulator of central functions for S. aureus lifestyle and pathogenicity....

  3. The Effect of Essential Oils on Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Seda Ozdikmenli

    2014-05-01

    Full Text Available Diseases caused by Staphylococcus aureus are widespread through the world in spite of developing technology. S. aureus is an important pathogen causing food intoxications besides hospital infections by its antibiotic resistant strains. Nowadays, there has been worldwide increasing concern on usage of natural products to control microorganisms. One of these natural products is essential oils. They are produced from plants especially from spices and composed of many components and volatiles. This review summarizes informative literature on essential oils and their mode of antimicrobial action. In addition, current knowledge on in vitro researches on antibacterial activity of essential oils and food applications to control S. aureus has been discussed.

  4. Staphylococcus aureus 'Down Under': contemporary epidemiology of S. aureus in Australia, New Zealand, and the South West Pacific.

    Science.gov (United States)

    Williamson, D A; Coombs, G W; Nimmo, G R

    2014-07-01

    The clinical and molecular epidemiology of Staphylococcus aureus disease has changed considerably over the past two decades, particularly with the emergence and spread of community-associated methicillin-resistant S. aureus (CA-MRSA) clones. Indeed, some of the first global descriptions of CA-MRSA were from remote indigenous communities in Western Australia, and from Pacific Peoples in New Zealand. The epidemiology of S. aureus infections in the South West Pacific has several unique features, largely because of the relative geographical isolation and unique indigenous communities residing in this region. In particular, a number of distinct CA-MRSA clones circulate in Australia and New Zealand, such as sequence type (ST) 93 methicillin-resistant S. aureus (MRSA) (Queensland clone) and clonal complex 75 S. aureus (Staphylococcus argenteus) in Australia, and ST30 MRSA (Southwest Pacific clone) in New Zealand. In addition, there is a disproportionate burden of S. aureus disease in indigenous paediatric populations, particularly in remote Aboriginal communities in Australia, and in Pacific Peoples and Maori in New Zealand. In this review, we provide a contemporary overview of the clinical and molecular epidemiology of S. aureus disease in the South West Pacific region, with a particular focus on features distinct to this region.

  5. Staphylococcus aureus intestinal colonization is associated with increased frequency of S. aureus on skin of hospitalized patients

    Directory of Open Access Journals (Sweden)

    Donskey Curtis J

    2007-09-01

    Full Text Available Abstract Background Intestinal colonization by Staphylococcus aureus among hospitalized patients has been associated with increased risk of staphylococcal infection and could potentially contribute to transmission. We hypothesized that S. aureus intestinal colonization is associated with increased frequency of S. aureus on patients' skin and nearby environmental surfaces. Methods Selected inpatients were cultured weekly for S. aureus from stool, nares, skin (groin and axilla, and environmental surfaces (bed rail and bedside table. Investigator's hands were cultured after contacting the patients' skin and the environmental surfaces. Results Of 71 subjects, 32 (45.1% had negative nares and stool cultures, 23 (32.4% had positive nares and stool cultures, 13 (18.3% were nares carriers only, and 3 (4.2% were stool carriers only. Of the 39 patients with S. aureus carriage, 30 (76.9% had methicillin-resistant isolates. In comparison to nares colonization only, nares and intestinal colonization was associated with increased frequency of positive skin cultures (41% versus 77%; p = 0.001 and trends toward increased environmental contamination (45% versus 62%; p = 0.188 and acquisition on investigator's hands (36% versus 60%; p = 0.057. Patients with negative nares and stool cultures had low frequency of S. aureus on skin and the environment (4.8% and 11.3%, respectively. Conclusion We found that hospitalized patients with S. aureus nares and/or stool carriage frequently had S. aureus on their skin and on nearby environmental surfaces. S. aureus intestinal colonization was associated with increased frequency of positive skin cultures, which could potentially facilitate staphylococcal infections and nosocomial transmission.

  6. PRICING AND ASSESSING UNIT-LINKED INSURANCE CONTRACTS WITH INVESTMENT GUARANTEES

    Directory of Open Access Journals (Sweden)

    Ciumas Cristina

    2014-07-01

    Full Text Available One of the most interesting life insurance products to have emerged in recent years in the Romanian insurance market has been the unit-linked contract. Unit-linked insurance products are life insurance policies with investment component. A unit-linked life insurance has two important components: protection and investment. The protection component refers to the insured sum in case of the occurrence of insured risks and the investment component refers to the policyholders’ account that represents the present value of the units from the chosen investment funds. Due to the financial instability caused by the Global Crisis and the amplification of market competitiveness, insurers from international markets have started to incorporate guarantees in unit-linked products. So a unit- linked life insurance policy with an asset value guarantee is an insurance policy whose benefit payable on death or at maturity consists of the greater of some guaranteed amount and the value of the units from the investment funds. One of the most challenging issues concerns the pricing of minimum death benefit and maturity benefit guarantees and the establishing of proper reserves for these guarantees. Insurers granting guarantees of this type must estimate the cost and include the cost in the premium. An important component of the activity carried out by the insurance companies is the investment of the premiums paid by policyholders in various types of assets, in order to obtain higher yields than those guaranteed by the insurance contracts, while providing the necessary liquidity for the payment of insurance claims in case of occurrence of the assumed risks. So the guaranteed benefits can be broadly matched or immunized with various types of financial assets, especially with fixed-interest instruments. According to Romanian legislation which regulates the unit-linked life insurance market, unit-linked life insurance contracts pass most of the investment risk to the

  7. Simple method for correct enumeration of Staphylococcus aureus

    DEFF Research Database (Denmark)

    Haaber, J.; Cohn, M. T.; Petersen, A.;

    2016-01-01

    culture. When grown in such liquid cultures, the human pathogen Staphylococcus aureus is characterized by its aggregation of single cells into clusters of variable size. Here, we show that aggregation during growth in the laboratory standard medium tryptic soy broth (TSB) is common among clinical...... and laboratory S. aureus isolates and that aggregation may introduce significant bias when applying standard enumeration methods on S. aureus growing in laboratory batch cultures. We provide a simple and efficient sonication procedure, which can be applied prior to optical density measurements to give...... an accurate estimate of cellular numbers in liquid cultures of S. aureus regardless of the aggregation level of the given strain. We further show that the sonication procedure is applicable for accurate determination of cell numbers using agar plate counting of aggregating strains....

  8. Improving Diagnosis and Treatment of Staphylococcus aureus Infections : Experimental Studies

    NARCIS (Netherlands)

    S. van den Berg (Sanne)

    2015-01-01

    markdownabstract__Abstract__ Staphylococcus aureus is an opportunistic pathogen that causes a variety of infections, ranging from mild skin infections like furuncles and impetigo, to severe, lifethreatening infections including endocarditis, osteomyelitis and pneumonia. Invasive infections are freq

  9. Rapid, Culture-Free Detection of Staphylococcus aureus Bacteremia

    Science.gov (United States)

    Burghardt, Elliot L.; Flenker, Katie S.; Clark, Karen C.; Miguel, Jeff; Ince, Dilek; Winokur, Patricia; Ford, Bradley; McNamara, James O.

    2016-01-01

    S. aureus bacteremia (SAB) is a common condition with high rates of morbidity and mortality. Current methods used to diagnose SAB take at least a day, and often longer. Patients with suspected bacteremia must therefore be empirically treated, often unnecessarily, while assay results are pending. In this proof-of-concept study, we describe an inexpensive assay that detects SAB via the detection of micrococcal nuclease (an enzyme secreted by S. aureus) in patient plasma samples in less than three hours. In total, 17 patient plasma samples from culture-confirmed S. aureus bacteremic individuals were tested. 16 of these yielded greater nuclease assay signals than samples from uninfected controls or individuals with non-S. aureus bacteremia. These results suggest that a nuclease-detecting assay may enable the rapid and inexpensive diagnosis of SAB, which is expected to substantially reduce the mortality and morbidity that result from this condition. PMID:27305148

  10. Staphylococcus aureus nasal carriage in hemodialysis centers of Fez, Morocco.

    Directory of Open Access Journals (Sweden)

    Idrissa Diawara

    2014-06-01

    Full Text Available Staphylococcus aureus (S. aureus nasal carriage may be responsible for some serious infections in hemodialyzed patients. The main target of this study was to estimate the prevalence of S. aureus nasal carriage in hemodialysis outpatients and medical staff in hemodialysis centers specifically in Fez region. The second target is to identify the risks of colonization, resistance pattern of isolates and their virulence toxin genes.Nasal swab specimens were obtained from 143 hemodialyzed outpatients and 32 medical staff from January to June 2012. Each participant completed a short questionnaire. Nasal carriage of S. aureus was demographically related (age, gender, hemodialysis duration, comorbidity (diabetes, malignancy and exposure to health care (dialysis staff, hospitalization. PCR (Polymerase Chain Reaction were used on all the isolates in the research of twelve staphylococcal enterotoxins genes. Also the PCR was used to investigate on the three factors epidermal cell differentiation inhibitors; three exfoliatin toxins; two leukotoxins; the toxic shock syndrome toxin-1 and the hemolysin beta genes.Nasal screening revealed 38.16%, 50% and 18.75% S. aureus carries in chronic, acute hemodialysis patients and medical staff, respectively. Only young participants were likely to be S. aureus carries (p = 0.002. But there were no gender differences between the isolate carriers and non-carriers or some comorbidity factors such as viral hepatitis B and C, HIV (Human Immunodeficiency Virus infections, diabetes, chronic smoking, recent hospitalization or antibiotic therapy. Out of all isolates, only one (1.61% was methicillin-resistant and Twenty-one (33.87% had at least two virulence toxin genes.Knowledge and monitoring of antibiotic resistance profile and virulence of S. aureus carriage are essential in the treatment of infections generated by this pathogen, as well as in the control of clonal dissemination and prevent the spread of S. aureus resistance.

  11. Comparative Pharmacodynamics of Gentamicin against Staphylococcus aureus and Pseudomonas aeruginosa†

    OpenAIRE

    Tam, Vincent H.; Kabbara, Samer; Vo, Giao; Schilling, Amy N.; Coyle, Elizabeth A.

    2006-01-01

    Aminoglycosides are often used to treat severe infections with gram-positive organisms. Previous studies have shown concentration-dependent killing by aminoglycosides of gram-negative bacteria, but limited data are available for gram-positive bacteria. We compared the in vitro pharmacodynamics of gentamicin against Staphylococcus aureus and Pseudomonas aeruginosa. Five S. aureus strains were examined (ATCC 29213 and four clinical isolates). Time-kill studies (TKS) in duplicate (baseline inocu...

  12. AKTIVITAS ANTIBAKTERI KITOSAN TERHADAP BAKTERI S.aureus

    OpenAIRE

    Mardiyah Kurniasih; Dwi Kartika

    2009-01-01

    Chitosan is the N-deacetylated derivative of chitin. Chitosan is biodegradable, biocompatible and non-toxic. Chitosan is polycationic in acidic media and give antibacterial activity. In this paper, antibacterial activity of chitosan have been studied. Chitosan had been isolated from white shrimp. Antibacterial activity of chitosan solutions was examined against S. aureus The result showed that antimicrobial effect on S. aureus was strengthened as the choitosan concentrate decreased.

  13. Long-term mortality after Staphylococcus aureus spondylodiscitis

    DEFF Research Database (Denmark)

    Aagaard, Theis; Roed, Casper; Larsen, Anders R;

    2014-01-01

    Patients diagnosed with Staphylococcus aureus spondylodiscitis have increased long-term mortality compared with the background population mainly due to infectious, endocrine, cardiovascular, gastrointestinal and alcohol and drug abuse-related diseases.......Patients diagnosed with Staphylococcus aureus spondylodiscitis have increased long-term mortality compared with the background population mainly due to infectious, endocrine, cardiovascular, gastrointestinal and alcohol and drug abuse-related diseases....

  14. Role of Monocytes in Experimental Staphylococcus aureus Endocarditis

    OpenAIRE

    Veltrop, Marcel H. A. M.; Bancsi, Maurice J. L. M. F.; Bertina, Rogier M.; Thompson, Jan

    2000-01-01

    In the pathogenesis of bacterial endocarditis (BE), the clotting system plays a cardinal role in the formation and maintenance of the endocardial vegetations. The extrinsic pathway is involved in the activation of the coagulation pathway with tissue factor (TF) as the key protein. Staphylococcus aureus is a frequently isolated bacterium from patients with BE. We therefore investigated whether S. aureus can induce TF activity (TFA) on fibrin-adherent monocytes, used as an in vitro model of BE....

  15. Vancomycin Resistance Pattern of Staphylococcus Aureus among Clinical Samples

    OpenAIRE

    S Saadat; K Solhjoo; A. Kazemi; Erfanian, S. (MSc); Ashrafian, F. (MSc)

    2014-01-01

    Background and Objective: Vancomycin is used for treatment of methicillin-resistant S. Aureus (MRSA) infections; therefore, resistance to this antibiotic is increasing. We aimed to determine the antibiotic resistance pattern and frequency of vancomycin resistant S. Areas (VRSA) strains isolated from clinical samples. Material and Methods: In this cross-sectional study, 100 S. Aureus isolates collected from hospitals in Shiraz during six months, 2012, were identified by biochemical, microbiolo...

  16. Staphylococcus aureus in the community: colonization versus infection.

    Directory of Open Access Journals (Sweden)

    Maureen Miller

    Full Text Available BACKGROUND: Antibiotic-resistant Staphylococcus aureus infections have increased dramatically in the community, yet S. aureus nasal colonization has remained stable. The objectives of this study were to determine if S. aureus colonization is a useful proxy measure to study disease transmission and infection in community settings, and to identify potential community reservoirs. METHODOLOGY/PRINCIPAL FINDINGS: Randomly selected households in Northern Manhattan, completed a structured social network questionnaire and provided nasal swabs that were typed by pulsed field gel electrophoresis to identify S. aureus colonizing strains. The main outcome measures were: 1 colonization with S. aureus; and 2 recent serious skin infection. Risk factor analyses were conducted at both the individual and the household levels; logistic regression models identified independent risks for household colonization and infection. RESULTS: 321 surveyed households contained 914 members. The S. aureus prevalence was 25% and MRSA was 0.4%. More than 40% of households were colonized. Recent antibiotic use was the only significant correlate for household colonization (p = .002. Seventy-eight (24% households reported serious skin infection. In contrast with colonization, five of the six risk factors that increased the risk of skin infection in the household at the univariate level remained independently significant in multivariable analysis: international travel, sports participation, surgery, antibiotic use and towel sharing. S. aureus colonization was not significantly associated with serious skin infection in any analysis. Among multiperson households with more than one person colonized, 50% carried the same strain. CONCLUSIONS/SIGNIFICANCE: The lack of association between S. aureus nasal colonization and serious skin infection underscores the need to explore alternative venues or body sites that may be crucial to transmission. Moreover, the magnitude of colonization and

  17. Staphylococcus aureus Peptidoglycan Tertiary Structure from Carbon-13 Spin Diffusion

    OpenAIRE

    Sharif, Shasad; Singh, Manmilan; Kim, Sung Joon; Schaefer,Jacob

    2009-01-01

    The cell-wall peptidoglycan of Staphylococcus aureus is a heterogeneous, highly cross-linked polymer of unknown tertiary structure. We have partially characterized this structure by measuring spin diffusion from 13C labels in pentaglycyl cross-linking segments to natural-abundance 13C in the surrounding intact cell walls. The measurements were performed using a version of centerband-only detection of exchange (CODEX). The cell walls were isolated from S. aureus grown in media containing [1-13...

  18. Resistance to Antimicrobials Mediated by Efflux Pumps in Staphylococcus aureus

    OpenAIRE

    Isabel Couto; Leonard Amaral; José Melo-Cristino; Miguel Viveiros; Cláudia Palma; Elisabete Junqueira; Costa, Sofia S.

    2013-01-01

    Resistance mediated by efflux has been recognized in Staphylococcus aureus in the last few decades, although its clinical relevance has only been recognized recently. The existence of only a few studies on the individual and overall contribution of efflux to resistance phenotypes associated with the need of well-established methods to assess efflux activity in clinical isolates contributes greatly to the lack of solid knowledge of this mechanism in S. aureus. This study aims to provide inform...

  19. Methicillin resistance & inducible clindamycin resistance in Staphylococcus aureus

    OpenAIRE

    Soumyadeep Ghosh; Mandira Banerjee

    2016-01-01

    Background & objectives: Methicillin resistant Staphylococcus aureus (MRSA) isolates with inducible clindamycin resistance (iCR) are resistant to erythromycin and sensitive to clindamycin on routine testing and inducible clindamycin resistance can only be identified by D-test. This study was aimed to detect methicillin resistance and iCR among S. aureus isolates, effectiveness of some commonly used antibiotics and correlation between methicillin resistance and iCR. Methods: The present cro...

  20. Detection and characterization of mupirocin resistance in Staphylococcus aureus.

    OpenAIRE

    Janssen, D A; Zarins, L T; Schaberg, D R; Bradley, S. F.; Terpenning, M S; Kauffman, C A

    1993-01-01

    Fourteen mupirocin-resistant Staphylococcus aureus strains were isolated over 18 months; 12 exhibited low-level resistance, while two showed high-level resistance. Highly mupirocin-resistant strains contained a large plasmid which transferred mupirocin resistance to other S. aureus strains and to Staphylococcus epidermidis. This plasmid and pAM899-1, a self-transferable gentamicin resistance plasmid, have molecular and biologic similarities.

  1. Methicillin-Resistant Staphylococcus aureus Ocular Infection in Taiwan

    OpenAIRE

    Kang, Yu-Chuan; Hsiao, Ching-Hsi; Yeh, Lung-Kun; Ma, David H. K.; Chen, Phil Y. F.; Lin, Hsin-Chiung; Tan, Hsin-Yuan; Chen, Hung-Chi; Chen, Shin-Yi; Huang, Yhu-Chering

    2015-01-01

    Abstract Methicillin-resistant Staphylococcus aureus (MRSA) infection is an important public health issue. This observational study aimed to characterize clinical features, antibiotic susceptibility, and genotypes of ocular infections caused by MRSA based on the clinical and molecular definitions of community-associated (CA) and healthcare-associated (HA) strains. Fifty-nine patients with culture-proven S aureus ocular infection were enrolled from January 1, 2010 to December 31, 2011 at Chang...

  2. Detoxification of toxins by bacillithiol in Staphylococcus aureus.

    Science.gov (United States)

    Newton, Gerald L; Fahey, Robert C; Rawat, Mamta

    2012-04-01

    Bacillithiol (BSH), an α-anomeric glycoside of l-cysteinyl-d-glucosaminyl-l-malate, is a major low-molecular-mass thiol found in bacteria such as Bacillus sp., Staphylococcus aureus and Deinococcus radiodurans. Like other low-molecular-mass thiols such as glutathione and mycothiol, BSH is likely to be involved in protection against environmental toxins including thiol-reactive antibiotics. We report here a BSH-dependent detoxification mechanism in S. aureus. When S. aureus Newman strain was treated with monobromobimane and monochlorobimane, the cellular BSH was converted to the fluorescent S-conjugate BS-bimane. A bacillithiol conjugate amidase activity acted upon the BS-bimane to produce Cys-bimane, which was then acetylated by an N-acetyltransferase to generate N-acetyl-Cys-bimane, a mercapturic acid. An S. aureus mutant lacking BSH did not produce mercapturic acid when treated with monobromobimane and monochlorobimane, confirming the involvement of bacillithiol. Furthermore, treatment of S. aureus Newman with rifamycin, the parent compound of the first-line anti-tuberculosis drug, rifampicin, indicated that this thiol-reactive antibiotic is also detoxified in a BSH-dependent manner, since mercapturic acids of rifamycin were observed in the culture medium. These data indicate that toxins and thiol-reactive antibiotics are detoxified to less potent mercapturic acids in a BSH-dependent manner and then exported out of the cell in S. aureus.

  3. Exploring the transcriptome of Staphylococcus aureus in its natural niche.

    Science.gov (United States)

    Chaves-Moreno, Diego; Wos-Oxley, Melissa L; Jáuregui, Ruy; Medina, Eva; Oxley, Andrew Pa; Pieper, Dietmar H

    2016-01-01

    Staphylococcus aureus is an important human pathogen and commensal, where the human nose is the predominant reservoir. To better understand its behavior in this environmental niche, RNA was extracted from the anterior nares of three documented S. aureus carriers and the metatranscriptome analyzed by RNAseq. In addition, the in vivo transcriptomes were compared to previously published transcriptomes of two in vitro grown S. aureus strains. None of the in vitro conditions, even growth in medium resembling the anterior nares environment, mimicked in vivo conditions. Survival in the nose was strongly controlled by the limitation of iron and evident by the expression of iron acquisition systems. S. aureus populations in different individuals clearly experience different environmental stresses, which they attempt to overcome by the expression of compatible solute biosynthetic pathways, changes in their cell wall composition and synthesis of general stress proteins. Moreover, the expression of adhesins was also important for colonization of the anterior nares. However, different S. aureus strains also showed different in vivo behavior. The assessment of general in vivo expression patterns and commonalities between different S. aureus strains will in the future result in new knowledge based strategies for controlling colonization. PMID:27641137

  4. Resistance to Antimicrobials Mediated by Efflux Pumps in Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Isabel Couto

    2013-03-01

    Full Text Available Resistance mediated by efflux has been recognized in Staphylococcus aureus in the last few decades, although its clinical relevance has only been recognized recently. The existence of only a few studies on the individual and overall contribution of efflux to resistance phenotypes associated with the need of well-established methods to assess efflux activity in clinical isolates contributes greatly to the lack of solid knowledge of this mechanism in S. aureus. This study aims to provide information on approaches useful to the assessment and characterization of efflux activity, as well as contributing to our understanding of the role of efflux to phenotypes of antibiotic resistance and biocide tolerance in S. aureus clinical isolates. The results described show that efflux is an important contributor to fluoroquinolone resistance in S. aureus and suggest it as a major mechanism in the early stages of resistance development. We also show that efflux plays an important role on the reduced susceptibility to biocides in S. aureus, strengthening the importance of this long neglected resistance mechanism to the persistence and proliferation of antibiotic/biocide-resistant S. aureus in the hospital environment.

  5. Determining of antibiotic resistance profile inStaphylococcus aureus isolates

    Institute of Scientific and Technical Information of China (English)

    Hossein Motamedi; Hadis Mirzabeigi; Tahere Shirali

    2010-01-01

    Objective:To determine the pattern of antibiotic resistance amongStaphylococcus aureus (S. aureus) isolates from clinical specimens and to identify community-acquired methicillin-resistantStaphylococcus aureus(CA-MRSA)in specimens that have been collected from patients referring to one of the hospitals of Ahvaz.Methods:S. aureus isolates from a hospital in Ahvaz were screened for resistance to various antibiotics including methicillin. The susceptibility of the isolates was determined by Kirby-Bauer disc diffusion method. TheMRSA was also treated with ethidium bromide to find the origin of resistance.Results: Among the bacterial isolates, all of 11S. aureus were resistant to methicillin and cefixime,2 were resistant to ciprofloxacine,6 were resistant to tetracycline and the reminder were sensitive or intermediate to other antibiotics. The treated isolates were reminded resistant to methicillin and this suggested that the plasmid was not the origin of resistance in these isolates.Conclusions: These results showed that infection due toMRSA is widespread in Ahvaz and with respect to the spread of vancomycin resistance among MRSA and appearance of overwhelming infections. It is necessary to identify continuously the profile of antibiotic resistance amongS. aureus isolates in other regions and finding appropriate antibiotic for infection control and eradication.

  6. Modelling the guaranteed QoS for wireless sensor networks: a network calculus approach

    Directory of Open Access Journals (Sweden)

    Yu Jianping

    2011-01-01

    Full Text Available Abstract Wireless sensor networks (WSNs became one of the high technology domains during the last 10 years. Real-time applications for them make it necessary to provide the guaranteed quality of service (QoS. The main contributions of this article are a system skeleton and a guaranteed QoS model that are suitable for the WSNs. To do it, we develop a sensor node model based on virtual buffer sharing and present a two-layer scheduling model using the network calculus. With the system skeleton, we develop a guaranteed QoS model, such as the upper bounds on buffer queue length/delay/effective bandwidth, and single-hop/multi-hops delay/jitter/effective bandwidth. Numerical results show the system skeleton and the guaranteed QoS model are scalable for different types of flows, including the self-similar traffic flows, and the parameters of flow regulators and service curves of sensor nodes affect them. Our proposal leads to buffer dimensioning, guaranteed QoS support and control in the WSNs.

  7. Prevalence of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus in Retail Ready-to-Eat Foods in China.

    Science.gov (United States)

    Yang, Xiaojuan; Zhang, Jumei; Yu, Shubo; Wu, Qingping; Guo, Weipeng; Huang, Jiahui; Cai, Shuzhen

    2016-01-01

    Staphylococcus aureus, particularly methicillin-resistant S.aureus (MRSA), is a life-threatening pathogen in humans, and its presence in food is a public health concern. MRSA has been identified in foods in China, but little information is available regarding MRSA in ready-to-eat (RTE) foods. We aimed to investigate the prevalence of S. aureus and MRSA in Chinese retail RTE foods. All isolated S. aureus were tested for antimicrobial susceptibility, and MRSA isolates were further characterized by multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing. Of the 550 RTE foods collected from 2011 to 2014, 69 (12.5%) were positive for S. aureus. Contamination levels were mostly in the range of 0.3-10 most probable number (MPN)/g, with five samples exceeding 10 MPN/g. Of the 69 S. aureus isolates, seven were identified as MRSA by cefoxitin disc diffusion test. Six isolates were mecA-positive, while no mecC-positive isolates were identified. In total, 75.8% (47/62) of the methicillin-susceptible S. aureus isolates and all of the MRSA isolates were resistant to three or more antibiotics. Amongst the MRSA isolates, four were identified as community-acquired strains (ST59-MRSA-IVa (n = 2), ST338-MRSA-V, ST1-MRSA-V), while one was a livestock-associated strain (ST9, harboring an unreported SCCmec type 2C2). One novel sequence type was identified (ST3239), the SCCmec gene of which could not be typed. Overall, our findings showed that Chinese retail RTE foods are likely vehicles for transmission of multidrug-resistant S. aureus and MRSA lineages. This is a serious public health risk and highlights the need to implement good hygiene practices.

  8. Execution of Fiduciary Guarantee Under Law No. 42 of 1999 on Fiduciary Guarantee (A Socio-Juridical Analysis to Anticipate Its Effectiveness

    Directory of Open Access Journals (Sweden)

    Arie Sukanti

    2013-09-01

    Full Text Available Fiduciary Guarantee (Fiduciary Law which approved by the House of Representatives of the Republic of Indonesia (DPR RI on September 9, 1999 has accommodate the public needs to help business activities and to provide legal certainty to the interested parties. With the increase in the development activities and the needs for funding, a majority of funds are needed to meet the lending and borrowing activities that require protection for the lender and the borrower through a guarantee institution that can provide legal certainty and protection to the lender or the borrower. Viewed from the current lending practices, there is a difficulty on the part of the Fiduciary Guarantee to conduct the fiduciary execution if the Fiduciary Grantor defaults since in fact the goods being a fiduciary object are still in the possession of the Fiduciary Grantor or Debtor, then in line with the provisions of article 1977 of the Indonesian Civil Code, known as the principle of bezit geldt als volkomen titel.

  9. Cooperative Cloud Service Aware Mobile Internet Coverage Connectivity Guarantee Protocol Based on Sensor Opportunistic Coverage Mechanism

    Directory of Open Access Journals (Sweden)

    Qin Qin

    2015-01-01

    Full Text Available In order to improve the Internet coverage ratio and provide connectivity guarantee, based on sensor opportunistic coverage mechanism and cooperative cloud service, we proposed the coverage connectivity guarantee protocol for mobile Internet. In this scheme, based on the opportunistic covering rules, the network coverage algorithm of high reliability and real-time security was achieved by using the opportunity of sensor nodes and the Internet mobile node. Then, the cloud service business support platform is created based on the Internet application service management capabilities and wireless sensor network communication service capabilities, which is the architecture of the cloud support layer. The cooperative cloud service aware model was proposed. Finally, we proposed the mobile Internet coverage connectivity guarantee protocol. The results of experiments demonstrate that the proposed algorithm has excellent performance, in terms of the security of the Internet and the stability, as well as coverage connectivity ability.

  10. Guaranteed cost control with constructing switching law of uncertain discrete-time switched systems

    Institute of Scientific and Technical Information of China (English)

    Zhang Ying; Duan Guangren

    2007-01-01

    A guaranteed cost control problem for a class of linear discrete-time switched systems with normbounded uncertainties is considered in this article. The purpose is to construct a switching rule and design a state feedback control law, such that, the closed-loop system is asymptotically stable and the closed-loop cost function value is not more than a specified upper bound for all admissible uncertainties under the constructed switching rule.A sufficient condition for the existence of guaranteed cost controllers and switching rules is derived based on the Lyapunov theory together with the linear matrix inequality (LMI) approach. Furthermore, a convex optimization problem with LMI constraints is formulated to select the suboptimal guaranteed cost controller. A numerical example demonstrates the validity of the proposed design approach.

  11. Guaranteed cost control with pole constraints for uncertain discrete-time switched systems

    Institute of Scientific and Technical Information of China (English)

    Ying ZHANG; Guangren DUAN; Liyan CHEN

    2009-01-01

    For a class of discrete-time switched systems with norm-bounded uncertainties and a quadratic cost index, the problem of designing a guaranteed cost state feedback controller with pole constraints is considered. A sufficient condition on the existence of robust guaranteed controllers is derived by a quadratic Lyapunov function approach together with linear matrix inequality (LMI)technique. Based on a constructed switching law, the closed-loop system is quadratic D-stable and the closedloop cost function value is not more than a specified upper bound. Furthermore, the design of suboptimal guaranteed cost controllers is turned into a convex optimization problem with linear matrix inequalities constraints. A numerical example demonstrates the effect of the proposed design approach.

  12. The structure and economic significance of government guarantees in Croatia and the European Union

    Directory of Open Access Journals (Sweden)

    Marko Primorac

    2016-03-01

    Full Text Available In the aftermath of the financial crisis, when countries are facing difficulties in raising the amounts of revenue needed to cover the expenditure side of the budget, fiscal risks can pose a significant threat to the sustainability of public finance. This became particularly evident in the case of public enterprises and their liabilities, which often increased public debt because of difficulties in meeting their financial obligations. The aim of this paper is to evaluate fiscal risks from government guarantees in Croatia and the European Union in general. Moreover, the paper aims to analyse the dynamics of the value and structure of government guarantees in Croatia in the period from 2009 to first half of 2015. Particular emphasis is placed on the impact of government guarantees on direct public debt in the context of methodological changes in the registration of public debt.

  13. Non-fragile guaranteed cost control for uncertain neutral large-scale interconnected systems

    Institute of Scientific and Technical Information of China (English)

    Dan Zhao; Qingling Zhang; Heli Hu; Chunyuan Zhao

    2010-01-01

    This paper focuses on the problem of non-fragile decentralized guaranteed cost control for uncertain neutral large-scale interconnected systems with time-varying delays in state,control input and interconnections.A novel scheme,viewing the interconnections with time-varying delays as effective information but not disturbances,is developed.Based on Lyapunov stability theory,using various techniques of decomposing and magnifying matrices,a design method of the non-fragile decentralized guaranteed cost controller for unperturbed neutral large-scale interconnected systems is proposed and the guaranteed cost is presented.The further results are derived for the uncertain case from the criterion of unperturbed neutral large-scale interconnected systems.Finally,an illustrative example shows that the results are significantly better than the existing results in the literatures.

  14. Performance Enhancement and Bandwidth Guarantee in IEEE 802.11 Wireless LANs

    Institute of Scientific and Technical Information of China (English)

    Yong Peng; Shi-Duan Cheng

    2004-01-01

    This paper first revisits the previously proposed NSAD (New Self-Adapt DCF) mechanism. Some modifications are presented to further enhance the performance of NSAD in the error-prone environment. Then a new MAC mechanism is proposed that can realize bandwidth guarantee by assigning different self-adapt parameters to users at different priority levels. The bandwidth guarantee property of this new mechanism is analyzed and the high priority users are found to have bandwidth guaranteed even in heavy contention condition, which is proved true not only by theoretical analysis but also by simulation results. At the same time the new scheme keeps the self-adapt character of NSAD, sothe overall system utilization is kept very high in heavy contention condition compared with the previously studied DCF-based QoS mechanisms.

  15. European beef consumers' interest in a beef eating-quality guarantee

    DEFF Research Database (Denmark)

    Verbeke, Wim; Van Wezemael, Lyn; de Barcellos, Marcia Dutra;

    2010-01-01

    Consumer demand in relation to food is increasingly shifting towards products that are safe, nutritious, and of good eating quality. Beef consumers are demanding for experience quality that matches their quality expectations formed prior to consumption, particularly with respect to beef tenderness...... from this study indicate good opportunities for the development of a beef eating-quality guarantee system in Europe. As an increase in consumers' satisfaction with beef products could lead to higher consumption rates and industry profitability, the introduction of an eating-quality guarantee system can....... The development of a beef quality grading and guarantee system, backed up by objective knowledge that is obtained through muscle profiling research, can allow the beef industry to meet these consumer demands. A qualitative consumer study has been carried out with beef consumers in France, Spain, United Kingdom...

  16. Legal Guarantees and Inconsistency of State Recognition to the Right of Religion/Belief in Indonesia

    OpenAIRE

    Kadarudin

    2015-01-01

    The right to freedom of religion/belief has been guaranteed by law in Indonesia, but there are still going on some violations of the right to freedom of religion/belief in the various regions. The purpose of this study was to determine the form of the legal guarantee of the right to freedom of religion/belief in Indonesia, shape inconsistencies state recognition of the right to religion/belief in Indonesia, and efforts to improve the rights of religion/belief in Indonesia. This paper uses a n...

  17. Time-optimal guaranteed search of mobile object in the plane

    Directory of Open Access Journals (Sweden)

    Avetisyan V.V.

    2015-03-01

    Full Text Available A problem of time-optimal guaranteed search of moving object with controllable speed in plane is observed, where in the initial moment only the circular of the sought object is known. The sought object is a object with controllable speed. The discovery, i.e. the identification of the precise coordinates of the object, is implemented through the moving information basis of the cone, the apex of which is connected to the current coordinates of the sough object. It is required to locate the object as quickly as possible. A control algorithm is proposed, in which r a guaranteed search of the sought object in minimal time is completed.

  18. Administrative Circular No. 13 (Rev. 4) - Guarantees for representatives of the personnel

    CERN Multimedia

    Department Head Office - HR Department

    2016-01-01

    Administrative Circular No. 13 (Rev. 4) entitled "Guarantees for representatives of the personnel", approved by the Director-General following discussion in the Standing Concertation Committee meeting on 22 March 2016, will be available on 1st September 2016 via the following link: https://cds.cern.ch/record/2208527.   This revised circular cancels and replaces Administrative Circular No. 13 (Rev. 3) also entitled "Guarantees for representatives of the personnel" of January 2014. This document contains a single change to reflect the terminology under the new career structure: the term "career path" is replaced by "grade". This circular will enter into force on 1st September 2016.

  19. Guaranteed Cost H∞ Controller Synthesis for Switched Systems Defined on Semi-algebraic Sets

    DEFF Research Database (Denmark)

    Ahmadi, Mohamadreza; Mojallali, Hamed; Wisniewski, Rafael

    2014-01-01

    A methodology to design guaranteed cost H∞ controllers for a class of switched systems with polynomial vector fields is proposed. To this end, we use sum of squares programming techniques. In addition, instead of the customary Carathéodory solutions, the analysis is performed in the framework...... feasibility tests leading to a stabilizing switching controller. Finally, we propose a scheme to synthesize stabilizing switching controllers with a guaranteed cost H∞ disturbance attenuation performance. The applicability of the proposed methods is elucidated thorough simulation analysis....

  20. Pathogenic Staphylococcus aureus Isolates from Postoperative Wounds of Hospitalized Patients

    Directory of Open Access Journals (Sweden)

    Smritikana Biswas

    2010-07-01

    Full Text Available Staphylococcus sp., gram positive pyogenic bacteria located on skin, nose etc, secretes toxin that causes toxic shock syndrome, abscess, food poisoning and other infectious diseases. This study was carried out to identify and characterize the type of Staphylococcus sp. bacteria especially Staphylococcus aureus in the pus from postoperative wounds of hospitalized patients. From pus samples collected from twenty-four patients from Kharagpur Hospital, Paschim Medinipur, West Bengal, twenty-eight bacterial isolates were obtained. Among them twenty-five (89.2% were appeared with golden yellow colonies which is usually formed by Staphylococcus aureus. Twenty-three (82.14% of the bacterial isolates were Gram positive. Among them twenty isolates (86.9% were further confirmed to be Staphylococcus aureus by their ability to produce Catalase enzyme (positive in Catalase test and Coagulase enzyme (positive in Coagulase Test. Eighteen (90.00% of these Staphylococcus aureus were found to liquefy gelatin (Gelatin hydrolysis test, were able to hydrolyze urea (Urea hydrolysis test and were also l positive in Mannitol Fermentation Test. But there was no growth found of these isolates on MacConkey Agar, while sixteen isolates (80.00% of Staphylococcus aureus were resistant to penicillin (50µg/ml. Moreover eighteen (90.00% Staphylococcus aureus isolates were able to elaborate Hemolysin (Hemolysis test on Blood Agar media. Hence the bacterial isolates obtained from pus of postoperative wounds were predominantly pathogenic Staphylococcus aureus. So it can be concluded that careful treatment and postoperative measures to be taken to avoid serious health problem that may often be life threatening.

  1. Staphylococcus aureus small colony variants in diabetic foot infections

    Directory of Open Access Journals (Sweden)

    Estrella Cervantes-García

    2015-03-01

    Full Text Available Background: Staphylococcus aureus (S. aureus is one of the major pathogens causing chronic infections. The ability of S. aureus to acquire resistance to a diverse range of antimicrobial compounds results in limited treatment options, particularly in methicillin-resistant S. aureus (MRSA. A mechanism by which S. aureus develops reduced susceptibility to antimicrobials is through the formation of small colony variants (SCVs. Infections by SCVs of S. aureus are an upcoming problem due to difficulties in laboratory diagnosis and resistance to antimicrobial therapy. Methods: A prospective study was performed on 120 patients diagnosed with both type 2 diabetes mellitus and infected diabetic foot ulcers. The study was carried out from July 2012 to December 2013 in Hospital General de Mexico. The samples were cultured in blood agar, mannitol salt agar, and MacConkey agar media, and incubated at 37°C in aerobic conditions. Results: We describe the first known cases of diabetic foot infections caused by MRSA-SCVs in patients diagnosed with type 2 diabetes mellitus and infected diabetic foot ulcers. In all of our cases, the patients had not received any form of gentamicin therapy. Conclusions: The antibiotic therapy commonly used in diabetic patients with infected diabetic foot ulcers fails in the case of MRSA-SCVs because the intracellular location protects S. aureus-SCVs from the host's defenses and also helps them resist antibiotics. The cases studied in this article add to the spectrum of persistent and relapsing infections attributed to MRSA-SCVs and emphasizes that these variants may also play a relevant role in diabetic foot infections.

  2. Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) at ambient freshwater beaches

    Science.gov (United States)

    Fogarty, Lisa R.; Haack, Sheridan K.; Johnson, Heather E.; Brennan, Angela K.; Isaacs, Natasha M.; Spencer, Chelsea

    2015-01-01

    Methicillin-resistant Staphylococcus aureus (MRSA) are a threat to human health worldwide, and although detected at marine beaches, they have been largely unstudied at freshwater beaches. Genes indicating S. aureus (SA; femA) and methicillin resistance (mecA) were detected at 11 and 12 of 13 US Great Lakes beaches and in 18% or 27% of 287 recreational water samples, respectively. Eight beaches had mecA + femA (potential MRSA) detections. During an intensive study, higher bather numbers, staphylococci concentrations, and femA detections were found in samples collected after noon than before noon. Local population density, beach cloud cover, and beach wave height were significantly correlated with SA or MRSA detection frequency. The Panton-Valentine leukocidin gene, associated with community-acquired MRSA, was detected in 12 out of 27 potential MRSA samples. The femA gene was detected less frequently at beaches that met US enterococci criteria or EU enterococci ‘excellent’ recreational water quality, but was not related to Escherichia coli-defined criteria. Escherichia coli is often the only indicator used to determine water quality at US beaches, given the economic and healthcare burden that can be associated with infections caused by SA and MRSA, monitoring of recreational waters for non-fecal bacteria such as staphylococci and/or SA may be warranted.

  3. A cohort study of the Copenhagen CF Centre eradication strategy against Staphylococcus aureus in patients with CF

    DEFF Research Database (Denmark)

    Dalbøge, Christina Schjellerup; Pressler, Tacjana; Høiby, Niels;

    2013-01-01

    Staphylococcus aureus is an important pathogen in CF. Centre prevalence of intermittent colonization and chronic S. aureus infections and the effectiveness of an anti-S. aureus eradication strategy was assessed....

  4. Guaranteed Cost Control for Uncertain Nonlinear Time-Delay Neutral Systems Based on T-S Fuzzy Model

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The problem of guaranteed cost fuzzy controller is studied for a class of nonlinear time-delay neutral systems with norm-bounded uncertainty based on T-S model. The sufficient conditions are first derived for the existence of guaranteed cost fuzzy controllers. These sufficient conditions are equivalent to a kind of linear matrix inequalities. Furthermore, a convex optimization problem with LMI constraints is formulated to design the optimal guaranteed cost controller.

  5. Alternative Labeling Programs and Purchasing Behavior toward Organic Foods: The Case of the Participatory Guarantee Systems in Brazil

    OpenAIRE

    Giovanna Sacchi; Vincenzina Caputo; Nayga, Rodolfo M.

    2015-01-01

    Regulatory standards and certification models are essential tools guaranteeing the authenticity of organic products. In particular, third-party certification is useful to consumers since it provides guarantees regarding production processes and food quality. In an attempt to cope with the costs and bureaucratic procedures related to the adoption of such certification, groups of small producers have begun to rely upon alternative quality assurance systems such as Participatory Guarantee System...

  6. Guaranteed capacity of hydro power plants in Germany and Austria; Gesicherte Leistung der Wasserkraft in Deutschland und Oesterreich

    Energy Technology Data Exchange (ETDEWEB)

    Pfleger, Markus [VERBUND Hydro Power GmbH, Wien (Austria); Ernst, Hans-Peter; Engels, Klaus [E.ON Kraftwerke GmbH, Sparte Wasserkraft, Landshut (Germany); Metzka, Rudolf [Technische Hochschule Deggendorf (Germany)

    2015-11-01

    Balancing fluctuations caused by renewable energy play an important role in the current transition of energy supply systems. Hydro power plants provide both: guaranteed capacity as well as renewability. In Germany and Austria definitions of how to quantify the guaranteed capacity of hydro power plants are well established to calculate the guaranteed capacity for an individual power plant. The definitions are challenged against real examples.

  7. Prevalence of Staphylococcus aureus in Shrimps in Tehran during 2013

    Directory of Open Access Journals (Sweden)

    Mohammad Mehdi Soltan Dallal

    2015-12-01

    Full Text Available Background During fishing and transport, preservation and quality of fish products are importantas well as storage to prevent the growth of pathogenic and toxin producing bacteria.Staphylococcus aureus is one of the most common causes of sea food-borne diseases worldwidedue to contamination of food by preformed enterotoxins. The aim of this study was to compare theprevalence and contamination of S. aureus in marine and farmed shrimps in Tehran fishery center.Methods: A total of 300 samples, including 150 marine, 150 farmed shrimps were selected duringSeptember 2013 to December 2013. Isolation and identification of S. aureus from isolated sampleswere carried out according to conventional methods, and antibiotic susceptibility test wasperformed by modified Kirby-Bauer disc diffusion methodResults: The results of this study showed that 30% of marine and 20% off armed shrimps werecontaminated with S. aureus. The highest resistance was observed with penicillin and ampicillin,whereas 100% were sensitive to vancomycin, clindamycin, ciprofloxacin, and rifampin.Conclusions: Due to relatively high contamination of shrimp by S. aureus more attention shouldbe given during processing and manufacturing.

  8. A systematic review of animal models for Staphylococcus aureus osteomyelitis

    Directory of Open Access Journals (Sweden)

    W Reizner

    2014-03-01

    Full Text Available Staphylococcus aureus (S. aureus osteomyelitis is a significant complication for orthopaedic patients undergoing surgery, particularly with fracture fixation and arthroplasty. Given the difficulty in studying S. aureus infections in human subjects, animal models serve an integral role in exploring the pathogenesis of osteomyelitis, and aid in determining the efficacy of prophylactic and therapeutic treatments. Animal models should mimic the clinical scenarios seen in patients as closely as possible to permit the experimental results to be translated to the corresponding clinical care. To help understand existing animal models of S. aureus, we conducted a systematic search of PubMed and Ovid MEDLINE to identify in vivo animal experiments that have investigated the management of S. aureus osteomyelitis in the context of fractures and metallic implants. In this review, experimental studies are categorised by animal species and are further classified by the setting of the infection. Study methods are summarised and the relevant advantages and disadvantages of each species and model are discussed. While no ideal animal model exists, the understanding of a model’s strengths and limitations should assist clinicians and researchers to appropriately select an animal model to translate the conclusions to the clinical setting.

  9. Methicillin-resistant Staphylococcus aureus in central Iowa wildlife.

    Science.gov (United States)

    Wardyn, Shylo E; Kauffman, Lin K; Smith, Tara C

    2012-10-01

    Livestock and pets have been identified as carriers of Staphylococcus aureus; however, the role of wild animals as a reservoir of S. aureus strains has not yet been examined. We conducted a pilot study to determine the prevalence of methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in 37 species of wild animals rehabilitated at a university clinic. Nasal, wing, wound, and cloacal swabs were collected. Of 114 animals, seven (6.1%) were MSSA-positive and three (2.6%) were MRSA-positive. The MRSA isolates were obtained from two eastern cottontail rabbits (Sylvilagus floridanus) and a Lesser Yellowlegs (Tringa flavipes), a migratory shorebird. Antibiotic resistance testing of the MRSA isolates revealed that two were additionally resistant to tetracycline and erythromycin, and the third isolate was also resistant to erythromycin, clindamycin, and levofloxacin. All three isolates were positive for the Panton-Valentine leukocidin (PVL) gene. Sequence typing of the staphylococcal protein A (spa) region revealed one MRSA isolate to be t002, whereas the other two MRSA isolates were found to be t008. Our results suggest that S. aureus, including MRSA, is being carried by wild animals, although at a low prevalence with the limited number of animals tested. Additional studies are needed to determine how this may impact human health. PMID:23060511

  10. Crystal Violet and XTT Assays on Staphylococcus aureus Biofilm Quantification.

    Science.gov (United States)

    Xu, Zhenbo; Liang, Yanrui; Lin, Shiqi; Chen, Dingqiang; Li, Bing; Li, Lin; Deng, Yang

    2016-10-01

    Staphylococcus aureus (S. Aureus) is a common food-borne pathogenic microorganism. Biofilm formation remains the major obstruction for bacterial elimination. The study aims at providing a basis for determining S. aureus biofilm formation. 257 clinical samples of S. aureus isolates were identified by routine analysis and multiplex PCR detection and found to contain 227 MRSA, 16 MSSA, 11 MRCNS, and 3 MSCNS strains. Two assays for quantification of S. aureus biofilm formation, the crystal violet (CV) assay and the XTT (tetrazolium salt reduction) assay, were optimized, evaluated, and further compared. In CV assay, most isolates formed weak biofilm 74.3 %), while the rest formed moderate biofilm (23.3 %) or strong biofilm (2.3 %). However, most isolates in XTT assay showed weak metabolic activity (77.0 %), while the rest showed moderate metabolic activity (17.9 %) or high metabolic activity (5.1 %). In this study, we found a distinct strain-to-strain dissimilarity in terms of both biomass formation and metabolic activity, and it was concluded from this study that two assays were mutual complementation rather than being comparison. PMID:27324342

  11. 13 CFR 107.820 - Financings in the form of guarantees.

    Science.gov (United States)

    2010-01-01

    ... guarantees. 107.820 Section 107.820 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES Financing of Small Businesses by Licensees Structuring Licensee's Financing... guaranty if: (1) You would become subject to State regulation as an insurance, guaranty or surety...

  12. 13 CFR 108.820 - Financings in the form of guarantees.

    Science.gov (United States)

    2010-01-01

    ... guarantees. 108.820 Section 108.820 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NEW MARKETS VENTURE CAPITAL (âNMVCâ) PROGRAM Financing of Small Businesses by NMVC Companies Structuring Nmvc Company.... You may not issue a guaranty if: (1) You would become subject to State regulation as an...

  13. Implementation of Guaranteed Services in the MANGO Clockless Network-on-Chip

    DEFF Research Database (Denmark)

    Bjerregaard, Tobias; Sparsø, Jens

    2006-01-01

    the effects of scaling microchip technologies. Equally important, a NoC facilitates a truly modular and scalable design flow. The MANGO (message-passing asynchronous network-on-chip providing guaranteed services over open core protocol (OCP) interfaces) NoC is presented, and how its key characteristics...

  14. 75 FR 17303 - Section 108 Community Development Loan Guarantee Program: Participation of States as Borrowers...

    Science.gov (United States)

    2010-04-06

    ... 800-877-8339. SUPPLEMENTARY INFORMATION: I. Background On July 22, 2009, at 74 FR 36384, HUD published..., interim rule implemented the expansion of section 108 loan guarantee authority provided by the 2009... obtain financing for eligible community development projects. Specifically, the final rule authorizes...

  15. Valuation of guaranteed annuity options using a stochastic volatility model for equity prices

    NARCIS (Netherlands)

    A. van Haastrecht; R. Plat; A. Pelsser

    2009-01-01

    Guaranteed Annuity Options are options providing the right to convert a policyholder’s accumulated funds to a life annuity at a fixed rate when the policy matures. These options were a common feature in UK retirement savings contracts issued in the 1970’s and 1980’s when interest rates were high, bu

  16. Fair Valuation of Guaranteed Contracts : The Interaction Between Assets and Liabilities

    NARCIS (Netherlands)

    Charlier, E.; Kleynen, R.H.M.A.

    2005-01-01

    In this paper we study the investment behavior of an insurance company consisting of policy and equity holders and issuing fair valued guaranteed contracts.Building up on the work of Briys and De Varenne (1997) and De Munnik and Schotman (1994) an intertemporal stochastic asset model and an interest

  17. AutoSyn:A new approach to automated synthesis of composite web services with correctness guarantee

    Institute of Scientific and Technical Information of China (English)

    HUAI JinPeng; DENG Ting; LI XianXian; DU ZongXia; GUO HuiPeng

    2009-01-01

    How to compose existing web services automatically and to guarantee the correctness of the design (e.g.freeness of deadlock and unspecified reception,and temporal constraints) is an important and challenging problem in web services.Most existing approaches require a detailed specification of the desired behaviors of a composite service beforehand and then perform certain formal verification to guarantee the correctness of the design,which makes the composition process both complex and time-consuming.In this paper,we propose a novel approach,referred to as AutoSyn to compose web services,where the correctness is guaranteed in the synthesis process.For a given set of services,a composite service is automatically constructed based on L* algorithm,which guarantees that the composite service is the most general way of coordinating services so that the correctness is ensured.We show the soundness and completeness of our solution and give a set of optimization techniques for reducing the time consumption.We have implemented a prototype system of AutoSyn and evaluated the effectiveness and efficiency of AutoSyn through an experimental study.

  18. 75 FR 13329 - Implications of Financial Accounting System (FAS) 166 on SBA Guaranteed Loan Programs

    Science.gov (United States)

    2010-03-19

    ... ADMINISTRATION Implications of Financial Accounting System (FAS) 166 on SBA Guaranteed Loan Programs AGENCY... Administration (SBA) is soliciting information and views from the public on: (1) The effect that the accounting changes mandated by the Financial Accounting Standards Board (FASB) in Financial Accounting Standard...

  19. 77 FR 54877 - Guaranteed Rural Rental Housing Low Loan-to-Cost Ratio

    Science.gov (United States)

    2012-09-06

    ... Rural Housing Service Guaranteed Rural Rental Housing Low Loan-to-Cost Ratio AGENCY: Rural Housing Service, USDA. ACTION: Notice. SUMMARY: The Rural Housing Service announces the low loan-to-cost ratio... U.S.C. 1490p-2) and operates under 7 CFR part 3565. The low loan-to- cost ratio is defined at...

  20. One in a Hundred – the third year of the development guarantee

    OpenAIRE

    Angelin, Anna

    2000-01-01

    Summary of report No 23. The Development Guarantee was introduced in Sweden January 1, 1998. It gives municipalities the opportunity to assume responsibility for young people between the ages of 20 to 24 for which the employment office cannot find employment within 90 days. 2000.

  1. Economic valuation of a toll road concession with traffic guarantees and the abandonment option

    Directory of Open Access Journals (Sweden)

    Frances Fischberg Blank

    2016-03-01

    Full Text Available Abstract Governments worldwide have been encouraging private participation in transportation infrastructure. To increase the feasibility of a project, public-private partnership (PPP may include guarantees or other support to reduce the risks for private investors. It is necessary to value these opportunities under a real options framework and thereby analyze the project's economic feasibility and risk allocation. However, within this structure, sponsors have an implicit option to abandon the project that should be simultaneously valued. Thus, this article proposes a hypothetical toll road concession in Brazil with a minimum traffic guarantee, a maximum traffic ceiling, and an implicit abandonment option. Different combinations of the minimum and maximum levels are presented, resulting in very high or even negative value added to the net present value (NPV. The abandonment option impacts the level of guarantee to be given. Governments should calibrate an optimal level of guarantees to avoid unnecessarily high costs, protect the returns of the sponsor, and lower the probability of abandonment.

  2. Enabling customer satisfaction and stock reduction through service differentiation with response time guarantees

    NARCIS (Netherlands)

    A.F. Gabor (Adriana); L.A. van Vianen (Lars); G. Yang (Guangyuan); S. Axsäter (Sven)

    2016-01-01

    textabstractIn response to customer specific service time guarantee requirements, service providers can offer differentiated services. However, conventional customer differentiation models based on fill rate constraints do not take full advantage of the stock reduction that can be achieved by differ

  3. Financial Audit: Guaranteed Student Loan Program's Internal Controls and Structure Need Improvement. Report to the Congress.

    Science.gov (United States)

    Wurtz, Donald R.; And Others

    An evaluative study was done of the Department of Education's system of internal accounting controls over the Federal Family Education Loan Program, known as the guaranteed student loan program. The study evaluated internal control systems, the structure of the program with respect to the role of guaranty agencies, and the Department's ability to…

  4. Balancing Tuition Predictability and Affordability: The Pitfalls of Guaranteed Tuition Plans

    Science.gov (United States)

    Delaney, Jennifer A.; Kearney, Tyler D.; Hemenway, Bradley

    2016-01-01

    As tuition levels rise, predictability is an increasingly important consideration of college financing. In this article, the authors explore contemporary policy tools intended to enhance tuition predictability. They specifically consider guaranteed tuition plans. The authors begin their discussion by considering the prevalence of guaranteed…

  5. 25 CFR 103.5 - What size loan will BIA guarantee or insure?

    Science.gov (United States)

    2010-04-01

    ... Indian, or more for an acceptable Indian business entity, Tribe, or tribal enterprise involving two or... 25 Indians 1 2010-04-01 2010-04-01 false What size loan will BIA guarantee or insure? 103.5 Section 103.5 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR FINANCIAL ACTIVITIES...

  6. LAND RECLAMATION IS BASIC GUARANTEE OF REGIONAL SUSTAINABLE DEVELOPMENT IN COAL MINING AREAS IN CHINA

    Institute of Scientific and Technical Information of China (English)

    张和生; 刘邦涛; 周丽霞; 崔春香

    1998-01-01

    Necessity of land reclamation is discussed, setting out viewpoint of sustainabledevelopment and land connotation and its attribute and combining destroyed forms andcharacteristics of land in coal mining areas. It is pointed out that land reclamation of coal miningareas is basic guarantee of solving contradiction between coal mining areas and countryside,raising life of resident and ensuring regional sustainable development.

  7. An Initial Econometric Consideration of Supply and Demand in the Guaranteed Student Loan Program.

    Science.gov (United States)

    Bayus, Barry; Kendis, Kurt

    1982-01-01

    In this econometric model of the Guaranteed Student Loan Program (GSLP), supply is related to banks' liquidity and yield curves, all lenders' economic costs and returns, and Student Loan Marketing Association activity. GSLP demand is based on loan costs, family debt position, and net student need for financial aid. (RW)

  8. Guaranteed Tax Base Formulas in School Finance: Can Equalization Be Achieved? Reply to Phelps and Addonizio.

    Science.gov (United States)

    Reilly, Gilbert J.

    1982-01-01

    Among the author's replies to the preceding article are that property tax relief programs do not solve interdistrict inequities under guaranteed tax base formulas, district income better measures fiscal capacity than does property wealth, and intrastate cost differentials do not remedy problems of low-income districts in high-income areas. (RW)

  9. GTB: An Aid in Understanding Connecticut's Guaranteed Tax Base Grant/School Finance Equalization Program.

    Science.gov (United States)

    Connecticut State Dept. of Education, Hartford.

    This booklet discusses the Horton v. Meskill case in which the Hartford, Connecticut, Superior Court ruled that the state's school financing system did not comply with state constitutional provisions concerning equal protection and education. It then outlines the legislature's reaction to the ruling--the guaranteed tax base (GTB)--and discusses…

  10. Wealth Neutrality Revisited: Guaranteed Tax Base Formulas and Local Choice in Public Education.

    Science.gov (United States)

    Reilly, Gilbert J.

    1982-01-01

    Proposes a model for evaluating state equalization aid formulas, accounting for municipal overburden and tradeoffs between expenditures and taxes. Tests the model using the Michigan state aid formula. Results relate elasticities to spending and tax levels and suggest that relatively unrestricted guaranteed tax base formulas can work. (PGD)

  11. Guaranteed Tax Base Formulas in School Finance: Why Equalization Doesn't Work.

    Science.gov (United States)

    Reilly, Gilbert J.

    1982-01-01

    Two shortcomings in the implementation of guaranteed tax base or equalization formulas--limitations on state equalization aid and inaccurate portrayal of fiscal capacity--can maintain or increase financial disparities among school districts. Data on Michigan districts illustrate how inaccurate fiscal measurement expanded existing disparities…

  12. An LMI Method to Robust Iterative Learning Fault-tolerant Guaranteed Cost Control for Batch Processes

    Institute of Scientific and Technical Information of China (English)

    WANG Limin; CHEN Xi; GAO Furong

    2013-01-01

    Based on an equivalent two-dimensional Fornasini-Marchsini model for a batch process in industry,a closed-loop robust iterative learning fault-tolerant guaranteed cost control scheme is proposed for batch processes with actuator failures.This paper introduces relevant concepts of the fault-tolerant guaranteed cost control and formulates the robust iterative learning reliable guaranteed cost controller (ILRGCC).A significant advantage is that the proposed ILRGCC design method can be used for on-line optimization against batch-to-batch process uncertainties to realize robust tracking of set-point trajectory in time and batch-to-batch sequences.For the convenience of implementation,only measured output errors of current and previous cycles are used to design a synthetic controller for iterative learning control,consisting of dynamic output feedback plus feed-forward control.The proposed controller can not only guarantee the closed-loop convergency along time and cycle sequences but also satisfy the H∞ performance level and a cost function with upper bounds for all admissible uncertainties and any actuator failures.Sufficient conditions for the controller solution are derived in terms of linear matrix inequalities (LMIs),and design procedures,which formulate a convex optimization problem with LMI constraints,are presented.An example of injection molding is given to illustrate the effectiveness and advantages of the ILRGCC design approach.

  13. Deterministic Price Setting Rules to Guarantee Profitability of Unbundling in the Airline Industry

    NARCIS (Netherlands)

    Van Diepen, G.; Curran, R.

    2011-01-01

    Unbundling the traditional airfare is one of the airline industry’s practices to generate ancillary revenue in its struggle for profitability. However, unbundling might just as well negatively affect profit. In this paper deterministic price setting rules are established to guarantee profitability o

  14. 75 FR 20257 - Amendment of the Temporary Liquidity Guarantee Program To Extend the Transaction Account...

    Science.gov (United States)

    2010-04-19

    ... qualify for the FDIC's guarantee and to continue to exercise risk- management principles applicable to an... deposit insurance limit) in qualifying noninterest-bearing transaction accounts. \\2\\ 73 FR 64179 (Oct. 29... assessment, ranging from 15 to 25 basis points based on an institution's deposit insurance assessment...

  15. 48 CFR 52.247-8 - Estimated Weights or Quantities Not Guaranteed.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Estimated Weights or... Provisions and Clauses 52.247-8 Estimated Weights or Quantities Not Guaranteed. As prescribed in 47.207-3(e... transportation-related services when weights or quantities are estimates: Estimated Weights or Quantities...

  16. A Unified Pricing of Variable Annuity Guarantees under the Optimal Stochastic Control Framework

    Directory of Open Access Journals (Sweden)

    Pavel V. Shevchenko

    2016-07-01

    Full Text Available In this paper, we review pricing of the variable annuity living and death guarantees offered to retail investors in many countries. Investors purchase these products to take advantage of market growth and protect savings. We present pricing of these products via an optimal stochastic control framework and review the existing numerical methods. We also discuss pricing under the complete/incomplete financial market models, stochastic mortality and optimal/sub-optimal policyholder behavior, and in the presence of taxes. For numerical valuation of these contracts in the case of simple risky asset process, we develop a direct integration method based on the Gauss-Hermite quadratures with a one-dimensional cubic spline for calculation of the expected contract value, and a bi-cubic spline interpolation for applying the jump conditions across the contract cashflow event times. This method is easier to implement and faster when compared to the partial differential equation methods if the transition density (or its moments of the risky asset underlying the contract is known in closed form between the event times. We present accurate numerical results for pricing of a Guaranteed Minimum Accumulation Benefit (GMAB guarantee available on the market that can serve as a numerical benchmark for practitioners and researchers developing pricing of variable annuity guarantees to assess the accuracy of their numerical implementation.

  17. 78 FR 66841 - Israel Loan Guarantees Issued Under the Emergency Wartime Supplemental Appropriations Act of 2003...

    Science.gov (United States)

    2013-11-07

    ..., including any individual, corporation, partnership, joint venture, association, joint stock company, trust... and conditions applicable to loan guarantees issued for the benefit of the Government of Israel on... issued for the benefit of the Government of Israel on behalf of the State of Israel...

  18. 13 CFR 120.210 - What percentage of a loan may SBA guarantee?

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What percentage of a loan may SBA guarantee? 120.210 Section 120.210 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS... percent, except as otherwise authorized by law....

  19. 7 CFR 1980.443 - Collateral, personal and corporate guarantees and other requirements.

    Science.gov (United States)

    2010-01-01

    ... flow to service its debts and meets key industry standards such as those of Robert Morris Associates... 7 Agriculture 14 2010-01-01 2009-01-01 true Collateral, personal and corporate guarantees and... (Continued) RURAL HOUSING SERVICE, RURAL BUSINESS-COOPERATIVE SERVICE, RURAL UTILITIES SERVICE, AND...

  20. 20 CFR 404.242 - Use of old-start primary insurance amount as guaranteed alternative.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Use of old-start primary insurance amount as guaranteed alternative. 404.242 Section 404.242 Employees' Benefits SOCIAL SECURITY ADMINISTRATION FEDERAL OLD-AGE, SURVIVORS AND DISABILITY INSURANCE (1950- ) Computing Primary Insurance Amounts...

  1. 78 FR 75215 - Qualified Mortgage Definition for HUD Insured and Guaranteed Single Family Mortgages

    Science.gov (United States)

    2013-12-11

    ... ``qualified mortgage'', HUD reviewed its mortgage insurance and loan guarantee programs and determined that all of the single family residential mortgage and loan products offered under HUD programs should be... borrower can repay the loan. For certain of its mortgage products, HUD establishes qualified...

  2. 76 FR 30904 - Rural Utilities Service Telecommunications Loan and Loan Guarantee Program

    Science.gov (United States)

    2011-05-27

    ... Rural Utilities Service Rural Utilities Service Telecommunications Loan and Loan Guarantee Program... telecommunications systems, equipment and services. The List of Materials historically has been a very useful tool in... to advancing state-of-the-art telecommunications technologies, consistent with our commitment to...

  3. Modeling on Guaranteed QoS for Wireless Sensor Networks: A Network Calculus Method

    CERN Document Server

    Zhang, Lianming; Deng, Xiaoheng

    2011-01-01

    Wireless sensor networks (WSNs) became one of the high-technology domains during the last years. Real-time applications for them make it necessary to provide the guaranteed Quality of Service (QoS). The main contributions of the paper are a service framework and a guaranteed QoS model that are suitable for the WSNs with some characteristics of the distribution, multi-hop, etc. To do it, we develop a sensor node model based on virtual buffer sharing and present a two-layer scheduling model by arrival curves and service curves in the network calculus. With this service framework, we develop a guaranteed QoS model, such as the upper bounds on buffer queue length, buffer queue delay, end-to-end delay/jitter and end-to-end effective bandwidth. Numerical results show that the service framework and guaranteed QoS model are highly scalable for different types of input flows, including the self-similar input flows, and they are only affected by the parameters of service curves and flow regulators. Our proposal leads t...

  4. 13 CFR 108.1600 - SBA authority to issue and guarantee Trust Certificates.

    Science.gov (United States)

    2010-01-01

    ... ADMINISTRATION NEW MARKETS VENTURE CAPITAL (âNMVCâ) PROGRAM SBA Financial Assistance for NMVC Companies (Leverage) Funding Leverage by Use of Sba Guaranteed Trust Certificates (âtcsâ) § 108.1600 SBA authority to issue and... of any TC. (b) SBA authority to arrange public or private fundings of Leverage. SBA in its...

  5. 13 CFR 107.1600 - SBA authority to issue and guarantee Trust Certificates.

    Science.gov (United States)

    2010-01-01

    ... ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES SBA Financial Assistance for Licensees (Leverage) Funding Leverage by Use of Sba-Guaranteed Trust Certificates (âtcsâ) § 107.1600 SBA authority to issue and... to arrange public or private fundings of Leverage. SBA in its discretion may arrange for public...

  6. Nonuniform covering method as applied to multicriteria optimization problems with guaranteed accuracy

    Science.gov (United States)

    Evtushenko, Yu. G.; Posypkin, M. A.

    2013-02-01

    The nonuniform covering method is applied to multicriteria optimization problems. The ɛ-Pareto set is defined, and its properties are examined. An algorithm for constructing an ɛ-Pareto set with guaranteed accuracy ɛ is described. The efficiency of implementing this approach is discussed, and numerical results are presented.

  7. INSURANCE GUARANTEE

    OpenAIRE

    Stanescu Catalin-Florinel; Simion Mircea-Laurentiu

    2013-01-01

    Providing security is designed to reduce uncertainty in the credit granting, offers a service or goods entrusted to a third persoane.Acest type of financial risk insurance is different from celelelte by the person who pays the insurance premium is not entitled direct insurance.

  8. Occurrence of Multidrug Resistant Staphylococcus aureus in horses in Malaysia

    Directory of Open Access Journals (Sweden)

    Z. Zunita

    2008-12-01

    Full Text Available A total of 22 Staphylococcus aureus were isolated from 50 samples from 8 stable horses. They are positive in the catalase and coagulase tests. Upon testing the cultures with SLIDEX test kit all formed agglutination within a few seconds, confirming they are of S. aureus. When cultured onto MSA, all isolates formed yellow colonies. However, none of the isolates produced blue colonies on ORSAB indicating that there were no MRSA among the S. aureus. There were 13 isolates which were multiresistant. Eleven are resistant to eight out of ten antibiotics tested. All these isolates were found to originate from stable G. One isolate is resistant to 5 antibiotics while another one isolate is resistant to 3 antibiotics. The rest of the isolates are not multiresistant to the antibiotics tested. [Veterinary World 2008; 1(6.000: 165-167

  9. Contribution of coagulases towards Staphylococcus aureus disease and protective immunity.

    Directory of Open Access Journals (Sweden)

    Alice G Cheng

    Full Text Available The bacterial pathogen Staphylococcus aureus seeds abscesses in host tissues to replicate at the center of these lesions, protected from host immune cells via a pseudocapsule. Using histochemical staining, we identified prothrombin and fibrin within abscesses and pseudocapsules. S. aureus secretes two clotting factors, coagulase (Coa and von Willebrand factor binding protein (vWbp. We report here that Coa and vWbp together are required for the formation of abscesses. Coa and vWbp promote the non-proteolytic activation of prothrombin and cleavage of fibrinogen, reactions that are inhibited with specific antibody against each of these molecules. Coa and vWbp specific antibodies confer protection against abscess formation and S. aureus lethal bacteremia, suggesting that coagulases function as protective antigens for a staphylococcal vaccine.

  10. Genetically enhanced cows resist intramammary Staphylococcus aureus infection.

    Science.gov (United States)

    Wall, Robert J; Powell, Anne M; Paape, Max J; Kerr, David E; Bannerman, Douglas D; Pursel, Vernon G; Wells, Kevin D; Talbot, Neil; Hawk, Harold W

    2005-04-01

    Mastitis, the most consequential disease in dairy cattle, costs the US dairy industry billions of dollars annually. To test the feasibility of protecting animals through genetic engineering, transgenic cows secreting lysostaphin at concentrations ranging from 0.9 to 14 micrograms/ml [corrected] in their milk were produced. In vitro assays demonstrated the milk's ability to kill Staphylococcus aureus. Intramammary infusions of S. aureus were administered to three transgenic and ten nontransgenic cows. Increases in milk somatic cells, elevated body temperatures and induced acute phase proteins, each indicative of infection, were observed in all of the nontransgenic cows but in none of the transgenic animals. Protection against S. aureus mastitis appears to be achievable with as little as 3 micrograms/ml [corrected] of lysostaphin in milk. Our results indicate that genetic engineering can provide a viable tool for enhancing resistance to disease and improve the well-being of livestock.

  11. Staphylococcus aureus biofilms: recent developments in biofilm dispersal.

    Science.gov (United States)

    Lister, Jessica L; Horswill, Alexander R

    2014-01-01

    Staphylococcus aureus is a major cause of nosocomial and community-acquired infections and represents a significant burden on the healthcare system. S. aureus attachment to medical implants and host tissue, and the establishment of a mature biofilm, play an important role in the persistence of chronic infections. The formation of a biofilm, and encasement of cells in a polymer-based matrix, decreases the susceptibility to antimicrobials and immune defenses, making these infections difficult to eradicate. During infection, dispersal of cells from the biofilm can result in spread to secondary sites and worsening of the infection. In this review, we discuss the current understanding of the pathways behind biofilm dispersal in S. aureus, with a focus on enzymatic and newly described broad-spectrum dispersal mechanisms. Additionally, we explore potential applications of dispersal in the treatment of biofilm-mediated infections.

  12. Synergistic antibacterial activity of Curcumin with antibiotics against Staphylococcus aureus.

    Science.gov (United States)

    Teow, Sin-Yeang; Ali, Syed Atif

    2015-11-01

    This study evaluated the synergistic antibacterial activity of Curcumin with 8 different antibiotic groups. Two reference, one clinical and ten environmental strains of Staphylococcus aureus (S. aureus) were tested. Disc diffusion assay with 25 μg/mL Curcumin demonstrated synergism in combination with a majority of tested antibiotics against S. aureus. However, checkerboard micro dilution assay only showed synergism, fractional inhibitory concentration index (FICI) indifferent interactions but no antagonism was observed. In time-kill curve, appreciable reduction of bacterial cells was also observed in combination therapy (Curcumin + antibiotics) compared to monotherapy (Curcumin or antibiotic(s) alone). The antibiotics with higher synergistic interaction with Curcumin are arranged in a decreasing order: Amikacin > Gentamicin > Ciprofloxacin.

  13. Quality control of direct molecular diagnostics for methicillin-resistant Staphylococcus aureus.

    NARCIS (Netherlands)

    A.F. van Belkum (Alex); H.G.M. Niesters (Bert); W.G. MacKay (William); W.B. van Leeuwen (Willem)

    2007-01-01

    textabstractTen samples containing various amounts of methicillin-resistant Staphylococcus aureus (MRSA), methicillin-susceptible S. aureus, methicillin-resistant Staphylococcus epidermidis (MRSE), and combinations thereof were distributed to 51 laboratories for molecular diagnostics testing. Sample

  14. Quality control of direct molecular diagnostics for methicillin-resistant Staphylococcus aureus

    NARCIS (Netherlands)

    van Belkum, Alex; Niesters, Hubert G M; MacKay, William G; van Leeuwen, Willem B

    2007-01-01

    Ten samples containing various amounts of methicillin-resistant Staphylococcus aureus (MRSA), methicillin-susceptible S. aureus, methicillin-resistant Staphylococcus epidermidis (MRSE), and combinations thereof were distributed to 51 laboratories for molecular diagnostics testing. Samples containing

  15. The Significance of Nasal Carriage of Staphylococcus Aureus and the Incidence of Postoperative Wound Infection

    NARCIS (Netherlands)

    R.P. Wenzel (Richard); T. M. Perl

    1995-01-01

    textabstractStaphylococcus aureus infections are associated with considerable morbidity and, in certain situations, mortality. The association between the nasal carriage of S. aureus and subsequent infection has been comprehensively established in a variety of clinical settings, in particular, patie

  16. Draft Genome Sequence of Methicillin-Sensitive Staphylococcus aureus ATCC 29213

    OpenAIRE

    Soni, Isha; Chakrapani, Harinath; Chopra, Sidharth

    2015-01-01

    Staphylococcus aureus subsp. aureus ATCC 29213 is one of the most commonly used strains in drug discovery research and for quality control. We report the completed draft genome sequence for the strain.

  17. Staphylococcus aureus resistance to topical antimicrobials in atopic dermatitis*

    Science.gov (United States)

    Bessa, Giancarlo Rezende; Quinto, Vanessa Petry; Machado, Daiane Corrêa; Lipnharski, Caroline; Weber, Magda Blessmann; Bonamigo, Renan Rangel; D'Azevedo, Pedro Alves

    2016-01-01

    Background Topical antimicrobial drugs are indicated for limited superficial pyodermitis treatment, although they are largely used as self-prescribed medication for a variety of inflammatory dermatoses, including atopic dermatitis. Monitoring bacterial susceptibility to these drugs is difficult, given the paucity of laboratory standardization. Objective To evaluate the prevalence of Staphylococcus aureus topical antimicrobial drug resistance in atopic dermatitis patients. Methods We conducted a cross-sectional study of children and adults diagnosed with atopic dermatitis and S. aureus colonization. We used miscellaneous literature reported breakpoints to define S. aureus resistance to mupirocin, fusidic acid, gentamicin, neomycin and bacitracin. Results A total of 91 patients were included and 100 S. aureus isolates were analyzed. All strains were methicillin-susceptible S. aureus. We found a low prevalence of mupirocin and fusidic acid resistance (1.1% and 5.9%, respectively), but high levels of neomycin and bacitracin resistance (42.6% and 100%, respectively). Fusidic acid resistance was associated with more severe atopic dermatitis, demonstrated by higher EASI scores (median 17.8 vs 5.7, p=.009). Our results also corroborate the literature on the absence of cross-resistance between the aminoglycosides neomycin and gentamicin. Conclusions Our data, in a southern Brazilian sample of AD patients, revealed a low prevalence of mupirocin and fusidic acid resistance of S. aureus atopic eczema colonizer strains. However, for neomycin and bacitracin, which are commonly used topical antimicrobial drugs in Brazil, high levels of resistance were identified. Further restrictions on the use of these antimicrobials seem necessary to keep resistance as low as possible.

  18. Highly sensitive detection of Staphylococcus aureus directly from patient blood.

    Directory of Open Access Journals (Sweden)

    Padmapriya P Banada

    Full Text Available BACKGROUND: Rapid detection of bloodstream infections (BSIs can be lifesaving. We investigated the sample processing and assay parameters necessary for highly-sensitive detection of bloodstream bacteria, using Staphylococcus aureus as a model pathogen and an automated fluidic sample processing-polymerase chain reaction (PCR platform as a model diagnostic system. METHODOLOGY/PRINCIPAL FINDINGS: We compared a short 128 bp amplicon hemi-nested PCR and a relatively shorter 79 bp amplicon nested PCR targeting the S. aureus nuc and sodA genes, respectively. The sodA nested assay showed an enhanced limit of detection (LOD of 5 genomic copies per reaction or 10 colony forming units (CFU per ml blood over 50 copies per reaction or 50 CFU/ml for the nuc assay. To establish optimal extraction protocols, we investigated the relative abundance of the bacteria in different components of the blood (white blood cells (WBCs, plasma or whole blood, using the above assays. The blood samples were obtained from the patients who were culture positive for S. aureus. Whole blood resulted in maximum PCR positives with sodA assay (90% positive as opposed to cell-associated bacteria (in WBCs (71% samples positive or free bacterial DNA in plasma (62.5% samples positive. Both the assays were further tested for direct detection of S. aureus in patient whole blood samples that were contemporaneous culture positive. S. aureus was detected in 40/45 of culture-positive patients (sensitivity 89%, 95% CI 0.75-0.96 and 0/59 negative controls with the sodA assay (specificity 100%, 95% CI 0.92-1. CONCLUSIONS: We have demonstrated a highly sensitive two-hour assay for detection of sepsis causing bacteria like S. aureus directly in 1 ml of whole blood, without the need for blood culture.

  19. Minimum inhibitory concentration of ciprofloxacin in combination with hexahydroquinoline derivatives against Staphylococcus aureus

    OpenAIRE

    F Amin Harati; Amini, M; AR Shahverdi; Pourmand MR; Yousefi, M

    2012-01-01

    Background: Staphylococcus aureus is the most common pathogen responsible for skin and soft tissue infections worldwide. Methicillin-resistant S. aureus is a major cause of both nosocomial and community acquired infections. The emergence of antimicrobial-resistant S. aureus is of global concern. Fluoroquinolone antimicrobials including ciprofloxacin, levofloxacin, and moxifloxacin are used to treat skin and soft tissue infections due to S. aureus. Emergence of ciprofloxacin resistance has inc...

  20. Investigation of the bactericidal effects of vancomycin and quinupristin/dalfopristin on Staphylococcus aureus isolates

    OpenAIRE

    HOŞGÖR-LİMONCU, Mine; ERMERTCAN, Şafak; COŞAR, Güner

    2004-01-01

    The present study aimed to determine the correlation between the bactericidal activity of vancomycin and quinupristin/dalfopristin (Q/D) on Staphylococcus aureus isolates and their minimal inhibition concentrations. The in-vitro susceptibilities of the 99 S. aureus isolates to vancomycin and Q/D were investigated by agar dilution. Thirty methicillin-resistant S. aureus (MRSA) and 30 methicillin-susceptible S. aureus (MSSA) vancomycin and Q/D susceptible isolates were involved in time-kill stu...

  1. Daptomycin-nonsusceptible, vancomycin-intermediate, methicillin-resistant Staphylococcus aureus endocarditis

    OpenAIRE

    Ryan Yu; Dale, Suzanne E; Deborah Yamamura; Vida Stankus; Christine Lee

    2012-01-01

    Due to the emergence of Staphylococcus aureus with reduced vancomycin susceptibility, newer antibiotics, including daptomycin, have been used to treat methicillin-resistant S aureus infections. Daptomycin is a cyclic lipopeptide that is approved to treat S aureus bacteremia and right-sided endocarditis, and reports of S aureus with reduced susceptibility to daptomycin are infrequent. To our knowledge, the present report describes the first Canadian case of daptomycin-nonsusceptible, vancomyci...

  2. In Vivo Activity of Ceftobiprole in Murine Skin Infections Due to Staphylococcus aureus and Pseudomonas aeruginosa▿

    OpenAIRE

    Fernandez, Jeffrey; Hilliard, Jamese J.; Abbanat, Darren; Zhang, Wenyan; Melton, John L.; Santoro, Colleen M.; Flamm, Robert K.; Bush, Karen

    2009-01-01

    Ceftobiprole, a broad-spectrum cephalosporin with activity against methicillin-resistant Staphylococcus aureus (MRSA) (P. Hebeisen et al., Antimicrob. Agents Chemother. 45:825-836, 2001), was evaluated in a subcutaneous skin infection model with Staphylococcus aureus Smith OC 4172 (methicillin-susceptible S. aureus [MSSA]), S. aureus OC 8525 (MRSA), Pseudomonas aeruginosa OC 4351 (having an inducible AmpC β-lactamase), and P. aeruginosa OC 4354 (overproducing AmpC β-lactamase). In the MSSA an...

  3. Genotyping of Staphylococcus aureus strains isolated from healthy persistent carriers.

    Science.gov (United States)

    Grzegorczyk, Agnieszka; Malm, Anna

    2014-07-01

    The paper presents results on the relatedness of Staphylococcus aureus strains colonizing the upper respiratory tract isolated from healthy persistent carriers. Genotyping was carried out using two methods--multiple-locus variable-number tandem-repeat fingerprinting (MLVF) and pulsed-field gel electrophoresis (PFGE). By comparison of the results obtained by both methods, good correlations between MLVF and PFGE genotyping of strains isolated from the asymptomatic carriers were observed. Further studies are needed to evaluate methods useful for genotyping of S. aureus strains circulating in the community. PMID:24488811

  4. Brain infection following experimental Staphylococcus aureus sepsis in pigs

    DEFF Research Database (Denmark)

    Astrup, Lærke Boye; Iburg, Tine Moesgaard; Nielsen, Ole Lerberg;

    2010-01-01

    Introduction: Sepsis is a major problem in humans and both the incidence and mortality is increasing. Multiple microabcesses can be found in the brain of septic patients. Staphylococcus aureus is one of the most common causes of sepsis and brain abscesses. S. aureus is also a frequent cause...... pigs were kept as controls. The pigs were euthanized in groups of four at either 6, 12, 24 or 48 h post infection. The brain was collected from all the animals and examined histologically. Results: All the inoculated pigs developed sepsis and 7 out of 12 animals had microabscesses in the prosencephalon...

  5. Mechanism of bacteriophage conversion of lipase activity in Staphylococcus aureus.

    OpenAIRE

    Lee, C Y; Iandolo, J J

    1985-01-01

    Staphylococcus aureus PS54 harbors two temperate bacteriophages and manifests no lipase activity on egg yolk agar. Curing of one of the resident prophages (L54a) restores lipase activity. To study the mechanism of bacteriophage conversion, the prophage was cured, and the gene encoding lipase activity was cloned into pBR322 in Escherichia coli on a 2.9-kilobase DNA fragment of the chromosome. The fragment was subcloned into a shuttle vector and subsequently transformed into S. aureus and Bacil...

  6. Threat of multidrug resistant Staphylococcus aureus in Western Nepal

    DEFF Research Database (Denmark)

    Bhatta, Dharm R.; Cavaco, Lina; Nath, Gopal;

    2015-01-01

    ObjectiveTo determine the prevalence of methicillin resistant Staphylococcus aureus (MRSA) and antimicrobial susceptibility patterns of the isolates from Manipal Teaching Hospital, Pokhara, Nepal. MethodsThis study was conducted over a period of 11 months (September 2012–August 2013) at the Manipal...... using disc diffusion test by cefoxitin (30 μg) and oxacillin (1 μg) disc, further confirmation was done by detection of mecA gene using PCR. ResultsOut of 400 Staphylococcus aureus strains, 139 (34.75%) were found to be MRSA. Among the MRSA isolates, 74 (53.2%) were from inpatient departments, 58 (41...

  7. Response of Staphylococcus Aureus to a Spaceflight Analogue

    Science.gov (United States)

    Castro, S. L.; Ott, C. M.

    2010-01-01

    The decreased gravity of the spaceflight environment creates quiescent, low fluid shear conditions. This environment can impart considerable effects on the physiology of microorganisms as well as their interactions with potential hosts. Using the rotating wall vessel (RWV), as a spaceflight analogue, the consequence of low fluid shear culture on microbial pathogenesis has provided a better understanding of the risks to the astronaut crew from infectious microorganisms. While the outcome of low fluid shear culture has been investigated for several bacterial pathogens, little has been done to understand how this environmental factor affects Staphylococcus aureus. S. aureus is an opportunistic human pathogen which presents a high level of infection risk to the crew, as it has been isolated from both the space shuttle and International Space Station. Given that approximately forty percent of the population are carriers of the bacteria, eradication of this organism from in flight environments is impractical. These reasons have lead to us to assess the response of S. aureus to a reduced fluid shear environment. Culture in the RWV demonstrated that S. aureus grown under the low-shear condition had lower cell concentrations after 10 hours when compared to the control culture. Furthermore, the low-shear cultured bacteria displayed a reduction in carotenoid production, pigments responsible for their yellow/gold coloration. When exposed to various environmental stressors, post low-shear culture, a decrease in the ability to survive oxidative assault was observed compared to control cultures. The low fluid shear environment also resulted in a decrease in hemolysin secretion, a staphylococcal toxin responsible for red blood cell lysis. When challenged by the immune components present in human whole blood, low-shear cultured S. aureus demonstrated significantly reduced survival rates as compared to the control culture. Assays to determine the duration of these alterations

  8. CHARACTERISATION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ISOLATES FROM SHINGLES PATIENTS

    Directory of Open Access Journals (Sweden)

    Jasmine R. et al.

    2011-11-01

    Full Text Available Even after treating Shingles patients with antiviral drugs, they are found to suffer from secondary bacterial infections. With this background as a guide, we undertook an investigation to isolate the bacterial pathogens from the pus of Shingles patients. Among the isolates obtained during the one year study period, Staphylococcus aureus sp. was found to be multi drug resistant and hence it was chosen for the study. The antibiogram pattern of the methicillin resistant S. aureus was obtained, since this could serve as a tool for suggesting useful drugs.

  9. Staphylococcus aureus sternal osteomyelitis: a rare cause of chest pain

    Directory of Open Access Journals (Sweden)

    Kaur M

    2015-10-01

    Full Text Available Chest pain is a common presenting symptom with a broad differential. Life-threatening cardiac and pulmonary etiologies of chest pain should be evaluated first. However, it is critical to perform a thorough assessment for other sources of chest pain in order to limit morbidity and mortality from less common causes. We present a rare case of a previously healthy 45 year old man who presented with focal, substernal, reproducible chest pain and Staphylococcus aureus bacteremia who was later found to have primary Staphylococcus aureus sternal osteomyelitis.

  10. Methicillin-Resistant Staphylococcus aureus Colonization in Schoolteachers in Ontario

    Directory of Open Access Journals (Sweden)

    Beth A Hanselman

    2008-01-01

    Full Text Available A prospective study of methicillin-resistant Staphylococcus aureus (MRSA colonization was performed involving teachers at a science teachers’ conference in Toronto, Ontario. Nasal swabs and questionnaire data were collected from consenting individuals. MRSA colonization was identified in seven of 220 (3.2% participants. No colonized individuals reported recent contact with the health care system, antimicrobial therapy, residence with health care workers or previous MRSA infections. Methicillin-susceptible S aureus colonization was identified in 72 of 220 (33% individuals. The prevalence of MRSA colonization was higher than expected for a purportedly low-risk population.

  11. The population structure of Staphylococcus Aureus among general practice patients from The Netherlands.

    NARCIS (Netherlands)

    Donker, G.A.; Deurenberg, R.H.; Driessen, C.; Sebastian, S.; Nijs, S.; Stobberingh, E.E.

    2009-01-01

    To investigate the prevalence, the antibiotic resistance pattern and the population structure of Staphylococcus aureus, S. aureus isolates from the anterior nostrils of patients of general practitioners (GPs) were analysed. Insight into the S. aureus population structure is essential, as nasal carri

  12. Nosocomial Infections and Drug Susceptibility Patterns in Methicillin Sensitive and Methicillin Resistant Staphylococcus aureus

    OpenAIRE

    Sharma, Nitish Kumar; Garg, Raina; Baliga, Shrikala; Bhat K., Gopalkrishna

    2013-01-01

    Aim: Staphylococcus aureus is one of the leading causes of nosocomial infections and is known for its ability to develop resistance to antibiotics. The drug susceptibility pattern of Methicillin Sensitive S. aureus (MSSA) and Methicillin Resistant S. aureus (MRSA) may vary.

  13. Staphylococcus aureus Strains That are Hypersusceptible to Resistance Gene Transfer from Enterococci▿

    OpenAIRE

    Sung, Julia M.-L.; Lindsay, Jodi A

    2007-01-01

    We identified naturally occurring Staphylococcus aureus mutants of the restriction modification pathway SauI, including bovine lineage ST151. In a model of vancomycin resistance transfer from Enterococcus faecalis, ST151 isolates are 500 times more susceptible than human S. aureus isolates. The eradication of “hyperrecipient” strains may reduce the evolution of vancomycin-resistant S. aureus.

  14. Genome Sequences of Four Staphylococcus aureus Strains Isolated from Bovine Mastitis

    OpenAIRE

    Kant, Ravi; Taponen, Suvi; Koort, Joanna; Paulin, Lars; Åvall-Jääskeläinen, Silja; Palva, Airi

    2015-01-01

    Staphylococcus aureus is a major causative agent of mastitis in dairy cows. The pathogenicity of S. aureus may vary; it is able to cause severe clinical mastitis, but most often it is associated with chronic subclinical mastitis. Here, we present the genome assemblies of four S. aureus strains from bovine mastitis.

  15. Genome Sequences of Four Staphylococcus aureus Strains Isolated from Bovine Mastitis.

    Science.gov (United States)

    Kant, Ravi; Taponen, Suvi; Koort, Joanna; Paulin, Lars; Åvall-Jääskeläinen, Silja; Palva, Airi

    2015-01-01

    Staphylococcus aureus is a major causative agent of mastitis in dairy cows. The pathogenicity of S. aureus may vary; it is able to cause severe clinical mastitis, but most often it is associated with chronic subclinical mastitis. Here, we present the genome assemblies of four S. aureus strains from bovine mastitis. PMID:25908141

  16. 29 CFR 778.412 - Relationship between amount guaranteed and range of hours employee may be expected to work.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Relationship between amount guaranteed and range of hours... Compensation Which Includes Overtime Pay § 778.412 Relationship between amount guaranteed and range of hours... workweeks. In both Walling v. A. H. Belo Co., 316 U.S. 624 and Walling v. Halliburton Oil Well Cementing...

  17. 78 FR 56227 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-09-12

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this Transaction....

  18. 78 FR 78955 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-12-27

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... application for final commitment for a long-term loan or financial guarantee in excess of $100 million (as... specified below will be presented to the Ex-Im Bank Board of Directors prior to final action on...

  19. 78 FR 69850 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-11-21

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this Transaction....

  20. 78 FR 42776 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-07-17

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this...

  1. 78 FR 23763 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-04-22

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this...

  2. 77 FR 47382 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2012-08-08

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this Transaction....

  3. 78 FR 76614 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-12-18

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this Transaction....

  4. 78 FR 16852 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-03-19

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this...

  5. 20 CFR 404.233 - Adjustment of your guaranteed alternative when you become entitled after age 62.

    Science.gov (United States)

    2010-04-01

    ... when you become entitled after age 62. 404.233 Section 404.233 Employees' Benefits SOCIAL SECURITY... Guaranteed Alternative for People Reaching Age 62 After 1978 But Before 1984 § 404.233 Adjustment of your guaranteed alternative when you become entitled after age 62. (a) If you do not become entitled to...

  6. 75 FR 3197 - Notice of a Public Meeting on Administration of the Business and Industry Guaranteed Loan Program

    Science.gov (United States)

    2010-01-20

    ... Rural Business-Cooperative Service Notice of a Public Meeting on Administration of the Business and Industry Guaranteed Loan Program AGENCY: Rural Business-Cooperative Service, USDA. ACTION: Notice of public... Development Mission area, will hold a public meeting entitled ``Business and Industry Guaranteed Loan...

  7. Staphylococcus aureus α toxin potentiates opportunistic bacterial lung infections.

    Science.gov (United States)

    Cohen, Taylor S; Hilliard, Jamese J; Jones-Nelson, Omari; Keller, Ashley E; O'Day, Terrence; Tkaczyk, Christine; DiGiandomenico, Antonio; Hamilton, Melissa; Pelletier, Mark; Wang, Qun; Diep, Binh An; Le, Vien T M; Cheng, Lily; Suzich, JoAnn; Stover, C Kendall; Sellman, Bret R

    2016-03-01

    Broad-spectrum antibiotic use may adversely affect a patient's beneficial microbiome and fuel cross-species spread of drug resistance. Although alternative pathogen-specific approaches are rationally justified, a major concern for this precision medicine strategy is that co-colonizing or co-infecting opportunistic bacteria may still cause serious disease. In a mixed-pathogen lung infection model, we find that the Staphylococcus aureus virulence factor α toxin potentiates Gram-negative bacterial proliferation, systemic spread, and lethality by preventing acidification of bacteria-containing macrophage phagosomes, thereby reducing effective killing of both S. aureus and Gram-negative bacteria. Prophylaxis or early treatment with a single α toxin neutralizing monoclonal antibody prevented proliferation of co-infecting Gram-negative pathogens and lethality while also promoting S. aureus clearance. These studies suggest that some pathogen-specific, antibody-based approaches may also work to reduce infection risk in patients colonized or co-infected with S. aureus and disparate drug-resistant Gram-negative bacterial opportunists.

  8. Genetic Diversity of Staphylococcus aureus in Buruli Ulcer

    NARCIS (Netherlands)

    Amissah, Nana Ama; Glasner, Corinna; Ablordey, Anthony; Tetteh, Caitlin S.; Kotey, Nana Konama; Prah, Isaac; van der Werf, Tjip; Rossen, John W.; van Dijl, Jan Maarten; Stienstra, Ymkje

    2015-01-01

    Background Buruli ulcer (BU) is a necrotizing skin disease caused by Mycobacterium ulcerans. Previous studies have shown that wounds of BU patients are colonized with M. ulcerans and several other microorganisms, including Staphylococcus aureus, which may interfere with wound healing. The present st

  9. Strain Discrimination of Staphylococcus aureus Using Superantigen Profiles.

    Science.gov (United States)

    Tsen, Hau-Yang; Li, Sheng-Chih; Chiang, Yu-Cheng; Tsai, Shuo-Wen

    2016-01-01

    Staphylococcus aureus is one of the major bacterial species that may cause clinical infection and food-poisoning cases. Strains of this species may produce a series of superantigens (SAgs). Due to the importance of staphylococcal infections, reliable methods for the discrimination of strains of this species are important. Such data may allow us to trace the infection origins and be used for epidemiological study. For strain discrimination, genotyping methods, such as pulsed-field gel electrophoresis (PFGE), random amplified polymorphic DNA (RAPD), and multi-locus sequence typing (MLST), etc., could be used. Recently, toxin gene profiles, which can be used for the elucidation of the genetic and pathogenic relatedness between strains, also have been used to improve the strain discrimination. For S. aureus, as more SAg genes were discovered, the SAg profiles become more useful for the strain discrimination of S. aureus. In this chapter, a method for the discrimination of S. aureus strains using superantigen profiles will be described in detail.

  10. USA300 Methicillin-resistant Staphylococcus aureus in Cuba

    Directory of Open Access Journals (Sweden)

    Hopman Joost

    2012-01-01

    Full Text Available Abstract Background Methicillin-resistant Staphylococcus aureus is an increasing problem in the Caribbean. We investigated the molecular epidemiology of MRSA isolates on Cuba. Findings The predominant clone was of the spa type t149, followed by community-associated MRSA USA300. Conclusions We report the first molecular typing results of MRSA isolates from Cuba.

  11. Vancomycin-resistant Staphylococcus aureus: no apocalypse now.

    Science.gov (United States)

    Goldstein, F W; Kitzis, M D

    2003-08-01

    The number of reports concerning vancomycin-resistant Staphylococcus aureus is much higher than the number of true resistant strains or unexpected clinical failures. Many confounding factors, including inadequate serum levels, severely ill patients, foreign devices or undrained abscesses, are more likely to be responsible for the clinical failures than resistance to vancomycin. PMID:14616695

  12. Methicillin-Resistant Staphylococcus aureus Colonization among Medical Residents

    Directory of Open Access Journals (Sweden)

    Pascale Trépanier

    2013-01-01

    Full Text Available BACKGROUND: Medical residents may be at risk of becoming colonized by methicillin-resistant Staphylococcus aureus (MRSA during their training. The occupational risk of this specific population is unknown. Furthermore, there are no data regarding MRSA colonization among health care professionals in Quebec.

  13. Human Staphylococcus aureus lineages among Zoological Park residents in Greece

    Directory of Open Access Journals (Sweden)

    E. Drougka

    2015-10-01

    Full Text Available Staphylococcus aureus is a part of the microbiota flora in many animal species. The clonal spread of S. aureus among animals and personnel in a Zoological Park was investigated. Samples were collected from colonized and infected sites among 32 mammals, 11 birds and eight humans. The genes mecA, mecC, lukF/lukS-PV (encoding Panton-Valentine leukocidin, PVL and tst (toxic shock syndrome toxin-1 were investigated by PCR. Clones were defined by Multilocus Sequence Typing (MLST, spa type and Pulsed-Field Gel Electrophoresis (PFGE. Seven S. aureus isolates were recovered from four animals and one from an employee. All were mecA, mecC and tst–negative, whereas, one carried the PVL genes and was isolated from an infected Squirrel monkey. Clonal analysis revealed the occurrence of seven STs, eight PFGE and five spa types including ones of human origin. Even though a variety of genotypes were identified among S. aureus strains colonizing zoo park residents, our results indicate that colonization with human lineages has indeed occurred.

  14. Killing of Staphylococcus aureus by C-8-Methoxy Fluoroquinolones

    OpenAIRE

    Zhao, Xilin; Wang, Jian-Ying; Xu, Chen; Dong, Yuzhi; Zhou, Jianfeng; Domagala, John; Drlica, Karl

    1998-01-01

    C-8-methoxy fluoroquinolones were more lethal than C-8-bromine, C-8-ethoxy, and C-8-H derivatives for Staphylococcus aureus, especially when topoisomerase IV was resistant. The methoxy group also increased lethality against wild-type cells when protein synthesis was inhibited. These properties encourage refinement of C-8-methoxy fluoroquinolones to kill staphylococci.

  15. Staphylococcus aureus with reduced susceptibility to vancomycin in healthcare settings.

    Science.gov (United States)

    Spagnolo, A M; Orlando, P; Panatto, D; Amicizia, D; Perdelli, F; Cristina, M L

    2014-12-01

    Glycopeptide resistance in Staphylococcus aureus is a source of great concern because, especially in hospitals, this class of antibiotics, particularly vancomycin, is one of the main resources for combating infections caused by methicillin-resistant Staphylococcus aureus strains (MRSA). Reduced susceptibility to vancomycin (VISA) was first described in 1996 in Japan; since then, a phenotype with heterogeneous resistance to vancomycin (h-VISA) has emerged. H-VISA isolates are characterised by the presence of a resistant subpopulation, typically at a rate of 1 in 10(5) organisms, which constitutes the intermediate stage betweenfully vancomycin-susceptible S. aureus (VSSA) and VISA isolates. As VISA phenotypes are almost uniformly cross-resistant to teicoplanin, they are also called Glycopeptides-intermediate Staphylococcus aureus strains (GISA) and, in the case of heterogeneous resistance to glycopeptides, h-GISA. The overall prevalence of h-VISA is low, accounting for approximately 1.3% of all MRSA isolates tested. Mortality due to h-GISA infections is very high (about 70%), especially among patients hospitalised in high-risk departments, such as intensive care units (ICU). Given the great clinical relevance of strains that are heteroresistant to glycopeptides and the possible negative impact on treatment choices, it is important to draw up and implement infection control practices, including surveillance, the appropriate use of isolation precautions, staff training, hand hygiene, environmental cleansing and good antibiotic stewardship.

  16. Review on Panton Valentine leukocidin toxin carriage among Staphylococcus aureus.

    Science.gov (United States)

    Shrestha, B

    2013-09-01

    Panton Valentine leukocidin is a toxin making pores in the polymorphonuclear cells which is a virulence factor of some strains of Staphylococcus aureus. Initially it was produced by methicillin susceptible Staphylococcus aureus only. Later with the acquisition of mecA gene has lead it to be PVL positive methicillin resistant Staphylococcus aureus. Since MRSA are resistant to many antibiotics and further they produce a toxin the infections by PVL positive MRSA has become a challenge. PVL positive MRSA a virulent strain of drug resistant superbug MRSA that has spread around the world, has claimed many lives in UK, Europe, USA and Australia. Some strains of superbug attack the healthy young people and kill within 24 hrs. PVL positive Staphylococcus aureus has been reported to be associated with skin and soft tissue infections however they also cause invasive infections and necrotizing pneumonia. These microorganisms known to be community associated have spread to hospitals. Hospital acquired infection by such microorganisms lead to an increase in mortality hence should be controlled before they become prevalent in hospitals. PMID:24908537

  17. Staphylococcus aureus causing tropical pyomyositis, Amazon Basin, Peru.

    NARCIS (Netherlands)

    Garcia, C.; Hallin, M.; Deplano, A.; Denis, O.; Sihuincha, M.; Groot, R. de; Gotuzzo, E.; Jacobs, J.

    2013-01-01

    We studied 12 Staphylococcus aureus isolates causing tropical pyomyositis in the Amazon Basin of Peru. All isolates were methicillin-susceptible; 11 carried Panton-Valentine leukocidin-encoding genes, and 5 belonged to multilocus sequence type 25 and possessed an extensive set of enterotoxins. Our f

  18. Epidemic Increase in Methicillin-resistant Staphylococcus aureus in Copenhagen

    DEFF Research Database (Denmark)

    Westh, Henrik; Boye, Kit; Bartels, Mette Damkjær;

    2006-01-01

    INTRODUCTION: We have found an epidemic increase in methicillin-resistant Staphylococcus aureus (MRSA) in Copenhagen. The increase has a complex background and involves hospitals, nursing homes and persons nursed in their own home. MATERIAL AND METHODS: We found 33 MRSA patients in 2003 and 121...

  19. Pyrazole Based Inhibitors against Enzymes of Staphylococcus aureus

    DEFF Research Database (Denmark)

    Jagadeesan, G.; Vijayakuma, Vinodhkumar; Palayam, Malathy;

    2015-01-01

    agents. The current study focuses on molecular docking and dynamics studies of pyrazole derivatives against Nucleosidase and DNA gyrase B of Staphylococcus aureus. Molecular docking and dynamics studies reveal that some of these derivatives show better binding abilities than some of the current drugs...

  20. Increased risk of arterial thromboembolic events after Staphylococcus aureus bacteremia

    DEFF Research Database (Denmark)

    Mejer, N; Gotland, N; Uhre, M L;

    2015-01-01

    OBJECTIVES: An association between infection and arterial thromboembolic events (ATE) has been suggested. Here we examined the risk of myocardial infarction (MI), stroke and other ATE after Staphylococcus aureus bacteremia (SAB). METHODS: Danish register-based nation-wide observational cohort study...

  1. An Interdisciplinary Experiment: Azo-Dye Metabolism by "Staphylococcus Aureus"

    Science.gov (United States)

    Brocklesby, Kayleigh; Smith, Robert; Sharp, Duncan

    2012-01-01

    An interdisciplinary and engaging practical is detailed which offers great versatility in the study of a qualitative and quantitative metabolism of azo-dyes by "Staphylococcus aureus". This practical has broad scope for adaptation in the number and depth of variables to allow a focused practical experiment or small research project. Azo-dyes are…

  2. Diabetes and risk of community-acquired Staphylococcus aureus bacteremia

    DEFF Research Database (Denmark)

    Smit, Jesper; Søgaard, Mette; Schønheyder, Henrik Carl;

    2016-01-01

    OBJECTIVE: Patients with diabetes may experience higher risk of Staphylococcus aureus bacteremia (SAB) than patients without diabetes due to decreased immunity or coexisting morbidities. We investigated the risk of community-acquired (CA) SAB in persons with and without diabetes. DESIGN: Using...

  3. Staphylococcus aureus with reduced susceptibility to vancomycin in healthcare settings.

    Science.gov (United States)

    Spagnolo, A M; Orlando, P; Panatto, D; Amicizia, D; Perdelli, F; Cristina, M L

    2014-12-01

    Glycopeptide resistance in Staphylococcus aureus is a source of great concern because, especially in hospitals, this class of antibiotics, particularly vancomycin, is one of the main resources for combating infections caused by methicillin-resistant Staphylococcus aureus strains (MRSA). Reduced susceptibility to vancomycin (VISA) was first described in 1996 in Japan; since then, a phenotype with heterogeneous resistance to vancomycin (h-VISA) has emerged. H-VISA isolates are characterised by the presence of a resistant subpopulation, typically at a rate of 1 in 10(5) organisms, which constitutes the intermediate stage betweenfully vancomycin-susceptible S. aureus (VSSA) and VISA isolates. As VISA phenotypes are almost uniformly cross-resistant to teicoplanin, they are also called Glycopeptides-intermediate Staphylococcus aureus strains (GISA) and, in the case of heterogeneous resistance to glycopeptides, h-GISA. The overall prevalence of h-VISA is low, accounting for approximately 1.3% of all MRSA isolates tested. Mortality due to h-GISA infections is very high (about 70%), especially among patients hospitalised in high-risk departments, such as intensive care units (ICU). Given the great clinical relevance of strains that are heteroresistant to glycopeptides and the possible negative impact on treatment choices, it is important to draw up and implement infection control practices, including surveillance, the appropriate use of isolation precautions, staff training, hand hygiene, environmental cleansing and good antibiotic stewardship. PMID:26137787

  4. Staphylococcus aureus redirects central metabolism to increase iron availability.

    Directory of Open Access Journals (Sweden)

    David B Friedman

    2006-08-01

    Full Text Available Staphylococcus aureus pathogenesis is significantly influenced by the iron status of the host. However, the regulatory impact of host iron sources on S. aureus gene expression remains unknown. In this study, we combine multivariable difference gel electrophoresis and mass spectrometry with multivariate statistical analyses to systematically cluster cellular protein response across distinct iron-exposure conditions. Quadruplicate samples were simultaneously analyzed for alterations in protein abundance and/or post-translational modification state in response to environmental (iron chelation, hemin treatment or genetic (Deltafur alterations in bacterial iron exposure. We identified 120 proteins representing several coordinated biochemical pathways that are affected by changes in iron-exposure status. Highlighted in these experiments is the identification of the heme-regulated transport system (HrtAB, a novel transport system which plays a critical role in staphylococcal heme metabolism. Further, we show that regulated overproduction of acidic end-products brought on by iron starvation decreases local pH resulting in the release of iron from the host iron-sequestering protein transferrin. These findings reveal novel strategies used by S. aureus to acquire scarce nutrients in the hostile host environment and begin to define the iron and heme-dependent regulons of S. aureus.

  5. Natural Population Dynamics and Carriage of Staphylococcus aureus

    NARCIS (Netherlands)

    D.C. Melles (Damian)

    2008-01-01

    textabstractStaphylococcus aureus is a major human pathogen capable of causing a wide range of infections, from relatively mild skin infections such as folliculitis and furunculosis to life-threatening conditions, including sepsis, deep abscesses, pneumonia, osteomyelitis, and infective endocarditis

  6. THE STUDY OF RESISTENCE OF STAPHYLOCOCCUS AUREUS STRAINS TO ANTIMICROBIALS

    Directory of Open Access Journals (Sweden)

    Nazarchuk GG

    2012-12-01

    Full Text Available In the research work the results of the study of resistance forming to antibiotics, antiseptics and decametoxine composition with modified polysaccharides in S.aureus strains are presented. The development of resistance to penicillins, cephalosporins, glycopeptides, macrolides is shown. Slow forming of resistance to decasan and decametoxine composition with carboxymethylamylum, oxyethylcellulose was determined.

  7. Low efficacy of tobramycin in experimental Staphylococcus aureus endocarditis

    DEFF Research Database (Denmark)

    Lerche, C. J.; Christophersen, L. J.; Trøstrup, H.;

    2015-01-01

    The empiric treatment of infective endocarditis (IE) varies widely and, in some places, a regimen of penicillin in combination with an aminoglycoside is administered. The increasing incidence of Staphylococcus aureus IE, poor tissue penetration by aminoglycosides and low frequency of penicillin...

  8. New insights into molecular typing methods for Staphylococcus aureus

    NARCIS (Netherlands)

    Ikawaty, R.

    2009-01-01

    Staphylococcus aureus (SA) remains a significant problem causing infections in both hospital and community settings. Methicillin-resistant SA (MRSA) continues to evolve and pose a great challenge through outbreaks and pandemic spread. Humans are no longer the only and the most important reservoir of

  9. Development Concept of Guaranteed Verification Electric Power System Simulation Tools and Its Realization

    Directory of Open Access Journals (Sweden)

    Gusev Alexander

    2015-01-01

    Full Text Available The analysis of existing problem reliability and verification of widespread electric power systems (EPS simulation tools is presented in this article. Everything simulation tools are based on the using of numerical methods for ordinary differential equations. Described the concept of guaranteed verification EPS simulation tools and the structure of its realization are based using the Simulator having properties of continuous , without decomposition three-phase EPS simulation in real time and on an unlimited range with guaranteed accuracy. The information from the Simulator can be verified by using data only quasi-steady-state regime received from the SCADA and such Simulator can be applied as the standard model for verification any EPS simulation tools.

  10. Optimal Guaranteed Service Time and Service Level Decision with Time and Service Level Sensitive Demand

    Directory of Open Access Journals (Sweden)

    Sangjun Park

    2014-01-01

    Full Text Available We consider a two-stage supply chain with one supplier and one retailer. The retailer sells a product to customer and the supplier provides a product in a make-to-order mode. In this case, the supplier’s decisions on service time and service level and the retailer’s decision on retail price have effects on customer demand. We develop optimization models to determine the optimal retail price, the optimal guaranteed service time, the optimal service level, and the optimal capacity to maximize the expected profit of the whole supply chain. The results of numerical experiments show that it is more profitable to determine the optimal price, the optimal guaranteed service time, and the optimal service level simultaneously and the proposed model is more profitable in service level sensitive market.

  11. Suboptimal reliable guaranteed cost control for continuous-time systems with multi-criterion constraints

    Institute of Scientific and Technical Information of China (English)

    Deng-feng ZHANG; Hong-ye SU; Jian CHU; Zhi-quan WANG

    2008-01-01

    The suboptimal reliable guaranteed cost control (RGCC) with multi-criterion constraints is investigated for a class of uncertain continuous-time systems with sensor faults.A fault model in sensors,which considers outage or partial degradation of sensors,is adopted.The influence of the disturbance on the quadratic stability of the closed-loop systems is analyzed.The reliable state-feedback controller is developed by a linear matrix inequalities (LMIs) approach,to minimize the upper bound of a quadratic cost function under the conditions that all the closed-loop poles be placed in a specified disk,and that the prescribed level of H∞ disturbance attenuation and the upper bound constraints of control inputs' magnitudes be guaranteed.Thus,with the above multi-criterion constraints,the resulting closed-loop system can provide satisfactory stability,transient property,a disturbance rejection level and mininaized quadratic cost performance despite possible sensor faults.

  12. Robust reliable guaranteed cost control for nonlinear singular stochastic systems with time delay

    Institute of Scientific and Technical Information of China (English)

    Zhang Aiqing; Fang Huajing

    2008-01-01

    To study the design problem of robust reliable guaranteed cost controller for nonlinear singular stochastic systems,the Takagi-Sugeno(T-S)fuzzy model is used to represent a nonlinear singular stochastic system with norm-bounded parameter uncertainties and time delay.Based on the linear matrix inequality(LMI)techniques and stability theory of stochastic differential equations,a stochastic Lyapunov function method is adopted to design a state feedback fuzzy controller.The resulting closed-loop fuzzy system is robustly reliable stochastically stable,and the corresponding quadratic cost function is guarauteed to be no more than a certain upper bound for all admissible uncertainties,as well as different actuator fault cases.A sufficient condition of existence and design method of robust reliable guaranteed cost controller is presented.Finally,a numerical simulation is given to illustrate the effectiveness of the proposed method.

  13. Guaranteed Cost Fault-Tolerant Control for Networked Control Systems with Sensor Faults

    Directory of Open Access Journals (Sweden)

    Qixin Zhu

    2015-01-01

    Full Text Available For the large scale and complicated structure of networked control systems, time-varying sensor faults could inevitably occur when the system works in a poor environment. Guaranteed cost fault-tolerant controller for the new networked control systems with time-varying sensor faults is designed in this paper. Based on time delay of the network transmission environment, the networked control systems with sensor faults are modeled as a discrete-time system with uncertain parameters. And the model of networked control systems is related to the boundary values of the sensor faults. Moreover, using Lyapunov stability theory and linear matrix inequalities (LMI approach, the guaranteed cost fault-tolerant controller is verified to render such networked control systems asymptotically stable. Finally, simulations are included to demonstrate the theoretical results.

  14. Improving the Quality of Service Guarantee in an Asynchronous Transfer Mode (ATM Network

    Directory of Open Access Journals (Sweden)

    Francis J. Ogwu

    2007-01-01

    Full Text Available This study presents a technique for improving the quality of service (QoS guarantee in an ATM network. In the proposed model, it was assumed that high priority traffic have been allocated a switch resource to guarantee a given QoS and low priority cells are allowed to enter the buffer, to improve the exploitation of reserved resources. The proposed technique was backed up with an exact analytical model for evaluating the cell loss probability of high and low priority cells. The performance of the proposed model was evaluated using C++ programming language. The results of the simulation shows that the loss probability of both high and low cells reduces as the buffer capacity increase and that the performance of high priority cell is better than that of low priority cell.

  15. GRS - Guarantee of results for grid-connected solar photovoltaic systems; GRS - Garantierte Resultate von Solarstromanlagen

    Energy Technology Data Exchange (ETDEWEB)

    Meier, Ch.; Frei, R.

    2001-07-01

    This final report for the Swiss Federal Office of Energy describes the development of a performance guarantee for the power delivered by grid-connected photovoltaic (PV) power plant. This is important for investors, who need to be able to calculate the price of the energy produced by the PV plant. The report examines the results of various case studies and experience gained with GRS contracts developed in different countries including Switzerland, the Netherlands, France and Germany. The problems encountered, which provided feedback for the further development of the contract presented in the report, are discussed. The model contract, which proposes a framework which provides many possible variations and adaptations, is presented in detail. Various problem areas such as guaranteed energy yield, meteorological references, correction methods, failure detection, dispute settlement and many other points are covered.

  16. Administrative Circular No. 13 (Rev. 3) - Guarantees for representatives of the personnel

    CERN Multimedia

    2014-01-01

    Administrative Circular No. 13 (Rev. 3) entitled “Guarantees for representatives of the personnel”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 5 December 2013 and entering into force on 1 January 2014, is available on the intranet site of the Human Resources Department (see here).   This circular is applicable to all members of the personnel. It cancels and replaces Administrative Circular No. 13 (Rev. 2) entitled “Guarantees for members of the personnel holding representative office” of November 1992. The circular was revised in order to adapt the time given to the representatives of the personnel to perform their elective mandate and to ensure more transparency in their activities, by indicating, inter alia, the percentage of time worked in the framework of their mandate, as well as the training, activities and ensuing results. Department Head Office HR Department

  17. Expression and inducibility in Staphylococcus aureus of the mecA gene, which encodes a methicillin-resistant S. aureus-specific penicillin-binding protein.

    OpenAIRE

    Ubukata, K; Nonoguchi, R; Matsuhashi, M; Konno, M

    1989-01-01

    A beta-lactam-sensitive strain of Staphylococcus aureus could be converted to methicillin resistance by the introduction of a plasmid carrying the 4.3-kilobase HindIII chromosomal DNA fragment which encoded the mecA gene from a methicillin-resistant S. aureus. Transformant cells produced methicillin-resistant S. aureus-specific penicillin-binding protein constitutively, and additional insertion of an inducible penicillinase plasmid caused production of the pencillin-binding protein to become ...

  18. Risk factors of nasal carriage of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus among health care staff in a teaching hospital in central Saudi Arabia

    OpenAIRE

    Al-Humaidan, Ohoud S.; El-kersh, Talat A; Al-Akeel, Raid A.

    2015-01-01

    Objectives: To investigate possible risk factors of Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA) nasal carriage associated with various health troubles among healthcare workers (HCWs) at King Khalid University Hospital (KKUH). Method: This prospective study was conducted between May 2012 and January 2013 in KKUH, Riyadh, Saudi Arabia. A total of 200 nasal swabs were collected from HCWs. Identification was carried out based on morphology, Gram stain, catalase an...

  19. Complex network perspective on structure and function of Staphylococcus aureus metabolic network

    Indian Academy of Sciences (India)

    L Ying; D W Ding

    2013-02-01

    With remarkable advances in reconstruction of genome-scale metabolic networks, uncovering complex network structure and function from these networks is becoming one of the most important topics in system biology. This work aims at studying the structure and function of Staphylococcus aureus (S. aureus) metabolic network by complex network methods. We first generated a metabolite graph from the recently reconstructed high-quality S. aureus metabolic network model. Then, based on `bow tie' structure character, we explain and discuss the global structure of S. aureus metabolic network. The functional significance, global structural properties, modularity and centrality analysis of giant strong component in S. aureus metabolic networks are studied.

  20. Investigations to guarantee the adminissible power densities in the core of pressurized power reactors

    International Nuclear Information System (INIS)

    Employing the computer code Garlic for simulation of the three-dimensional core behaviour and the control and limitation systems, analyses have been made in order to assess the effectiveness of the limiting actions to guarantee the admissible power densities in the core. Examples from normal and disturbed operating conditions have shown that the limitation systems are effective to observe the admissible operating region following the guidelines of the TUeV-Leitstelle Kerntechnik in all cases analyzed. (orig.)

  1. Mutual Loan-Guarantee Societies in Monopolistic Credit Markets with Adverse Selection

    OpenAIRE

    Giovanni Busetta; Alberto Zazzaro

    2009-01-01

    In many countries, Mutual Loan-Guarantee Societies (MLGSs) are assuming ever-increasing importance for small business lending. In this paper we provide a theory to rationalise the raison d'^etre of MLGSs. The basic intuition is that the foundation for MLGSs lies in the inefficiencies created by adverse selection, when borrowers do not have enough collateralisable wealth to satisfy collateral requirements and induce self-selecting contracts. In this setting, we view MLGSs as a wealth-pooling m...

  2. Justifying a revised voting rights act: The Guarantee Clause and the problem of minority rule

    OpenAIRE

    Chin, GJ

    2014-01-01

    In Shelby County v. Holder, the Supreme Court invalidated Section 4 of the Voting Rights Act of 1965, which required certain jurisdictions with histories of discrimination to "preclear" changes to their voting practices under Section 5 before those changes could become effective. This Article proposes that Congress ground its responsive voting rights legislation in the Constitution's Guarantee Clause, in addition to the Fourteenth and Fifteenth Amendments. The Court has made clear that the Gu...

  3. Horizon Adaptation for Nonlinear Model Predictive Controllers with guaranteed Degree of Suboptimality

    CERN Document Server

    Pannek, Jürgen

    2011-01-01

    We propose adaptation strategies to modify the standard constrained model predictive controller scheme in order to guarantee a certain lower bound on the degree of suboptimality. Within this analysis, the length of the optimization horizon is the parameter we wish to adapt. We develop and prove several shortening and prolongation strategies which also allow for an effective implementation. Moreover, extensions of stability results and suboptimality estimates to model predictive controllers with varying optimization horizon are shown.

  4. Implicit state guarantees exacerbate problem: Separated banking system alone not a solution

    OpenAIRE

    Klaus, Benjamin; Schäfer, Dorothea

    2013-01-01

    Many banks are now too big, complex, and closely interconnected to be liquidated. When they run into difficulties, they threaten the entire financial system of their economic area. Five years of financial crisis have not alleviated but exacerbated this problem. The cost of stabilizing banks is enormous, posing serious challenges to the states affected. In addition, such state guarantees create dangerously false incentives: they encourage managers and investors to engage into high risk-taking,...

  5. Do government loan guarantee instruments make sense? : evaluating effectiveness of the tranched cover fund

    OpenAIRE

    Viggiano, Claudio

    2016-01-01

    Small and Medium Enterprises (SMEs) have a fundamental role in advanced economies in generating employment and in fostering innovation, but they often struggle with lack of financing resources due to the presence of information asymmetries. Loan Guarantee Instruments are the most adopted schemes to mitigate market imperfections, allowing banks to shift some risk on the guarantor, thus leading to a less restrictive lending behavior. The overall aim of this thesis is to examine whether the T...

  6. A New Performance Guarantee for Orthogonal Matching Pursuit Using Mutual Coherence

    OpenAIRE

    Emadi, Mohammad; Miandji, Ehsan; Unger, Jonas; Afshari, Ehsan

    2016-01-01

    In this paper we present a new coherence-based performance guarantee for the Orthogonal Matching Pursuit (OMP) algorithm. An upper bound for the probability of correctly identifying the support of a sparse signal with additive white Gaussian noise is derived. Compared to previous work, the new bound takes into account the signal parameters such as dynamic range, noise variance, and sparsity. Numerical simulations show significant improvements over previous work.

  7. Mahanaxar: quality of service guarantees in high-bandwidth, real-time streaming data storage

    Energy Technology Data Exchange (ETDEWEB)

    Bigelow, David [Los Alamos National Laboratory; Bent, John [Los Alamos National Laboratory; Chen, Hsing-Bung [Los Alamos National Laboratory; Brandt, Scott [UCSC

    2010-04-05

    Large radio telescopes, cyber-security systems monitoring real-time network traffic, and others have specialized data storage needs: guaranteed capture of an ultra-high-bandwidth data stream, retention of the data long enough to determine what is 'interesting,' retention of interesting data indefinitely, and concurrent read/write access to determine what data is interesting, without interrupting the ongoing capture of incoming data. Mahanaxar addresses this problem. Mahanaxar guarantees streaming real-time data capture at (nearly) the full rate of the raw device, allows concurrent read and write access to the device on a best-effort basis without interrupting the data capture, and retains data as long as possible given the available storage. It has built in mechanisms for reliability and indexing, can scale to meet arbitrary bandwidth requirements, and handles both small and large data elements equally well. Results from our prototype implementation shows that Mahanaxar provides both better guarantees and better performance than traditional file systems.

  8. Guarantee Funds of Credits in Romania - Necessity, Features and Networking - II

    Directory of Open Access Journals (Sweden)

    Georgeta Dragomir

    2011-12-01

    Full Text Available In the current context, marked by financial crisis, economic recession, lack of liquidity andlow capitalization, the access tofinancing is more difficult, mainly due to the lack, insufficiency orlimited availability of guarantees. Guarantee funds represent an innovative solution, proven by theEuropean economies and recently launched in Romania. Taking over the international experience inorder to facilitate the SMEs’ access to financial resources has become a necessity in Romania, whichwas reflected by the creation of three specialized financial institutions in granting guarantees domain.These are bodies with private or publiccapital, which relate, based on agreements with commercialbanks. After a period of exposure to major risks from the lack of regulatory activity, the guaranteefunds, along with other non-banking financial institutions are absolutely governed by rules oftheNRB, according to the banking law and special laws. The analysis in this paper is based on the studyof several official documents, reports of specialized bodies, statistics and examples of good practice,summarized and interpreted in through comparison and developments. The aim is to identify anumber of issues that may lead to sensitize the responsible factors-public authorities, creditinstitutions, and specialists-to come up with new practical solutions that would stimulate thefinancing processof small entrepreneurs.

  9. Fuzzy Logic-Based Guaranteed Lifetime Protocol for Real-Time Wireless Sensor Networks.

    Science.gov (United States)

    Shah, Babar; Iqbal, Farkhund; Abbas, Ali; Kim, Ki-Il

    2015-08-18

    Few techniques for guaranteeing a network lifetime have been proposed despite its great impact on network management. Moreover, since the existing schemes are mostly dependent on the combination of disparate parameters, they do not provide additional services, such as real-time communications and balanced energy consumption among sensor nodes; thus, the adaptability problems remain unresolved among nodes in wireless sensor networks (WSNs). To solve these problems, we propose a novel fuzzy logic model to provide real-time communication in a guaranteed WSN lifetime. The proposed fuzzy logic controller accepts the input descriptors energy, time and velocity to determine each node's role for the next duration and the next hop relay node for real-time packets. Through the simulation results, we verified that both the guaranteed network's lifetime and real-time delivery are efficiently ensured by the new fuzzy logic model. In more detail, the above-mentioned two performance metrics are improved up to 8%, as compared to our previous work, and 14% compared to existing schemes, respectively.

  10. Entanglement guarantees emergence of cooperation in quantum prisoner's dilemma games on networks.

    Science.gov (United States)

    Li, Angsheng; Yong, Xi

    2014-09-05

    It was known that cooperation of evolutionary prisoner's dilemma games fails to emerge in homogenous networks such as random graphs. Here we proposed a quantum prisoner's dilemma game. The game consists of two players, in which each player has three choices of strategy: cooperator (C), defector (D) and super cooperator (denoted by Q). We found that quantum entanglement guarantees emergence of a new cooperation, the super cooperation of the quantum prisoner's dilemma games, and that entanglement is the mechanism of guaranteed emergence of cooperation of evolutionary prisoner's dilemma games on networks. We showed that for a game with temptation b, there exists a threshold arccos √b/b for a measurement of entanglement, beyond which, (super) cooperation of evolutionary quantum prisoner's dilemma games is guaranteed to quickly emerge, giving rise to stochastic convergence of the cooperations, that if the entanglement degree γ is less than the threshold arccos √b/b, then the equilibrium frequency of cooperations of the games is positively correlated to the entanglement degree γ, and that if γ is less than arccos √b/b and b is beyond some boundary, then the equilibrium frequency of cooperations of the games on random graphs decreases as the average degree of the graphs increases.

  11. FP/FIFO scheduling: coexistence of deterministic and probabilistic QoS guarantees

    Directory of Open Access Journals (Sweden)

    Pascale Minet

    2007-01-01

    Full Text Available In this paper, we focus on applications having quantitative QoS (Quality of Service requirements on their end-to-end response time (or jitter. We propose a solution allowing the coexistence of two types of quantitative QoS garantees, deterministic and probabilistic, while providing a high resource utilization. Our solution combines the advantages of the deterministic approach and the probabilistic one. The deterministic approach is based on a worst case analysis. The probabilistic approach uses a mathematical model to obtain the probability that the response time exceeds a given value. We assume that flows are scheduled according to non-preemptive FP/FIFO. The packet with the highest fixed priority is scheduled first. If two packets share the same priority, the packet arrived first is scheduled first. We make no particular assumption concerning the flow priority and the nature of the QoS guarantee requested by the flow. An admission control derived from these results is then proposed, allowing each flow to receive a quantitative QoS guarantee adapted to its QoS requirements. An example illustrates the merits of the coexistence of deterministic and probabilistic QoS guarantees.

  12. Guaranteeing QoS of media-based applications in virtualized environment

    Science.gov (United States)

    Zhou, Like; Wu, Song; Shi, Xuanhua; Jin, Hai; Zhou, Jiangfu

    2013-12-01

    With the rapid development of web technology and smart phone, multimedia contents spread all over the Internet. The prevalence of virtualization technology enables multimedia service providers to run media servers in virtualized servers or rented virtual machines (VMs) in a cloud environment. Although server consolidation using virtualization can substantially increase the efficient use of server resources, it introduces resources competition among VMs running different applications. Recently, hypervisors do not make any Quality of Service (QoS) guarantee for media-based applications if they are consolidated with other network-intensive applications, which leads to significant performance degradation. For example, Xen only offers a static method to allocate network bandwidth. In this paper, we find that the performance of media-based applications running in VMs degrades seriously when they are consolidated with other VMs running network-intensive applications and argues that dynamic network bandwidth allocation is essential to guarantee the QoS of media-based applications. Then, we present a dynamic network bandwidth allocation system in virtualized environment, which allocates network bandwidth dynamically and effectively, and does not interrupt running services in VMs. The experiments show that our system can not only guarantee the QoS of media-based applications well but also maximize the system's the overall performance while ensuring the QoS of media-based applications.

  13. Heme Recognition By a Staphylococcus Aureus IsdE

    Energy Technology Data Exchange (ETDEWEB)

    Grigg, J.C.; Vermeiren, C.L.; Heinrichs, D.E.; Murphy, M.E.P.

    2009-06-03

    Staphylococcus aureus is a Gram-positive bacterial pathogen and a leading cause of hospital acquired infections. Because the free iron concentration in the human body is too low to support growth, S. aureus must acquire iron from host sources. Heme iron is the most prevalent iron reservoir in the human body and a predominant source of iron for S. aureus. The iron-regulated surface determinant (Isd) system removes heme from host heme proteins and transfers it to IsdE, the cognate substrate-binding lipoprotein of an ATP-binding cassette transporter, for import and subsequent degradation. Herein, we report the crystal structure of the soluble portion of the IsdE lipoprotein in complex with heme. The structure reveals a bi-lobed topology formed by an N- and C-terminal domain bridged by a single {alpha}-helix. The structure places IsdE as a member of the helical backbone metal receptor superfamily. A six-coordinate heme molecule is bound in the groove established at the domain interface, and the heme iron is coordinated in a novel fashion for heme transporters by Met{sup 78} and His{sup 229}. Both heme propionate groups are secured by H-bonds to IsdE main chain and side chain groups. Of these residues, His{sup 299} is essential for IsdE-mediated heme uptake by S. aureus when growth on heme as a sole iron source is measured. Multiple sequence alignments of homologues from several other Gram-positive bacteria, including the human pathogens pyogenes, Bacillus anthracis, and Listeria monocytogenes, suggest that these other systems function equivalently to S. aureus IsdE with respect to heme binding and transport.

  14. Methicillin resistant S. aureus in human and bovine mastitis.

    Science.gov (United States)

    Holmes, Mark A; Zadoks, Ruth N

    2011-12-01

    Staphylococcus aureus is a ubiquitous organism that causes a variety of diseases including mastitis in cattle and humans. High-level resistance of S. aureus to β-lactams conferred by a mecA gene encoding a modified penicillin binding protein (PBP2a) was first observed in the early 1960's. These methicillin resistant S. aureus (MRSA) have been responsible for both hospital acquired infections (HA-MRSA) and, more recently, community acquired MRSA (CA-MRSA). A small number of human MRSA mastitis cases and outbreaks in maternity or neonatal units have been reported which are generally the result of CA-MRSA. The establishment of the sequence type 398 (ST398) in farm animals, primarily pigs, in the early 2000's has provided a reservoir of infection for humans and dairy cattle, particularly in continental Europe, described as livestock-associated MRSA (LA-MRSA). Prior to the emergence of ST398 there were sporadic reports of MRSA in bovine milk and cases of mastitis, often caused by strains from human associated lineages. Subsequently, there have been several reports describing bovine udder infections caused by ST-398 MRSA. Recently, another group of LA-MRSA strains was discovered in humans and dairy cattle in Europe. This group carries a divergent mecA gene and includes a number of S. aureus lineages (CC130, ST425, and CC1943) that were hitherto thought to be bovine-specific but are now also found as carriage or clinical isolates in humans. The emergence of MRSA in dairy cattle may be associated with contact with other host species, as in the case of ST398, or with the exchange of genetic material between S. aureus and coagulase negative Staphylococcus species, which are the most common species associated with bovine intramammary infections and commonly carry antimicrobial resistance determinants.

  15. Characterization of a mouse-adapted Staphylococcus aureus strain.

    Science.gov (United States)

    Holtfreter, Silva; Radcliff, Fiona J; Grumann, Dorothee; Read, Hannah; Johnson, Sarah; Monecke, Stefan; Ritchie, Stephen; Clow, Fiona; Goerke, Christiane; Bröker, Barbara M; Fraser, John D; Wiles, Siouxsie

    2013-01-01

    More effective antibiotics and a protective vaccine are desperately needed to combat the 'superbug' Staphylococcus aureus. While in vivo pathogenicity studies routinely involve infection of mice with human S. aureus isolates, recent genetic studies have demonstrated that S. aureus lineages are largely host-specific. The use of such animal-adapted S. aureus strains may therefore be a promising approach for developing more clinically relevant animal infection models. We have isolated a mouse-adapted S. aureus strain (JSNZ) which caused a severe outbreak of preputial gland abscesses among male C57BL/6J mice. We aimed to extensively characterize this strain on a genomic level and determine its virulence potential in murine colonization and infection models. JSNZ belongs to the MLST type ST88, rare among human isolates, and lacks an hlb-converting phage encoding human-specific immune evasion factors. Naive mice were found to be more susceptible to nasal and gastrointestinal colonization with JSNZ than with the human-derived Newman strain. Furthermore, naïve mice required antibiotic pre-treatment to become colonized with Newman. In contrast, JSNZ was able to colonize mice in the absence of antibiotic treatment suggesting that this strain can compete with the natural flora for space and nutrients. In a renal abscess model, JSNZ caused more severe disease than Newman with greater weight loss and bacterial burden. In contrast to most other clinical isolates, JSNZ can also be readily genetically modified by phage transduction and electroporation. In conclusion, the mouse-adapted strain JSNZ may represent a valuable tool for studying aspects of mucosal colonization and for screening novel vaccines and therapies directed at preventing colonization. PMID:24023720

  16. Characterization of a mouse-adapted Staphylococcus aureus strain.

    Directory of Open Access Journals (Sweden)

    Silva Holtfreter

    Full Text Available More effective antibiotics and a protective vaccine are desperately needed to combat the 'superbug' Staphylococcus aureus. While in vivo pathogenicity studies routinely involve infection of mice with human S. aureus isolates, recent genetic studies have demonstrated that S. aureus lineages are largely host-specific. The use of such animal-adapted S. aureus strains may therefore be a promising approach for developing more clinically relevant animal infection models. We have isolated a mouse-adapted S. aureus strain (JSNZ which caused a severe outbreak of preputial gland abscesses among male C57BL/6J mice. We aimed to extensively characterize this strain on a genomic level and determine its virulence potential in murine colonization and infection models. JSNZ belongs to the MLST type ST88, rare among human isolates, and lacks an hlb-converting phage encoding human-specific immune evasion factors. Naive mice were found to be more susceptible to nasal and gastrointestinal colonization with JSNZ than with the human-derived Newman strain. Furthermore, naïve mice required antibiotic pre-treatment to become colonized with Newman. In contrast, JSNZ was able to colonize mice in the absence of antibiotic treatment suggesting that this strain can compete with the natural flora for space and nutrients. In a renal abscess model, JSNZ caused more severe disease than Newman with greater weight loss and bacterial burden. In contrast to most other clinical isolates, JSNZ can also be readily genetically modified by phage transduction and electroporation. In conclusion, the mouse-adapted strain JSNZ may represent a valuable tool for studying aspects of mucosal colonization and for screening novel vaccines and therapies directed at preventing colonization.

  17. Population structure of Staphylococcus aureus from remote African Babongo Pygmies.

    Directory of Open Access Journals (Sweden)

    Frieder Schaumburg

    Full Text Available BACKGROUND: Pandemic community-acquired methicillin-resistant Staphylococcus aureus isolates (CA-MRSA predominantly encode the Panton-Valentine leukocidin (PVL, which can be associated with severe infections. Reports from non-indigenous Sub-Saharan African populations revealed a high prevalence of PVL-positive isolates. The objective of our study was to investigate the S. aureus carriage among a remote indigenous African population and to determine the molecular characteristics of the isolates, particularly those that were PVL-positive. METHODOLOGY/PRINCIPAL FINDINGS: Nasal S. aureus carriage and risk factors of colonization were systematically assessed in remote Gabonese Babongo Pygmies. Susceptibility to antibiotics, possession of toxin-encoding genes (i.e., PVL, enterotoxins, and exfoliative toxins, S. aureus protein A (spa types and multi-locus sequence types (MLST were determined for each isolate. The carriage rate was 33%. No MRSA was detected, 61.8% of the isolates were susceptible to penicillin. Genes encoding PVL (55.9%, enterotoxin B (20.6%, exfoliative toxin D (11.7% and the epidermal cell differentiation inhibitor B (11.7% were highly prevalent. Thirteen spa types were detected and were associated with 10 STs predominated by ST15, ST30, ST72, ST80, and ST88. CONCLUSIONS: The high prevalence of PVL-positive isolates among Babongo Pygmies demands our attention as PVL can be associated with necrotinzing infection and may increase the risk of severe infections in remote Pygmy populations. Many S. aureus isolates from Babongo Pygmies and pandemic CA-MRSA-clones have a common genetic background. Surveillance is needed to control the development of resistance to antibiotic drugs and to assess the impact of the high prevalence of PVL in indigenous populations.

  18. Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection.

    Directory of Open Access Journals (Sweden)

    Aisling F Brown

    Full Text Available Mechanisms of protective immunity to Staphylococcus aureus infection in humans remain elusive. While the importance of cellular immunity has been shown in mice, T cell responses in humans have not been characterised. Using a murine model of recurrent S. aureus peritonitis, we demonstrated that prior exposure to S. aureus enhanced IFNγ responses upon subsequent infection, while adoptive transfer of S. aureus antigen-specific Th1 cells was protective in naïve mice. Translating these findings, we found that S. aureus antigen-specific Th1 cells were also significantly expanded during human S. aureus bloodstream infection (BSI. These Th1 cells were CD45RO+, indicative of a memory phenotype. Thus, exposure to S. aureus induces memory Th1 cells in mice and humans, identifying Th1 cells as potential S. aureus vaccine targets. Consequently, we developed a model vaccine comprising staphylococcal clumping factor A, which we demonstrate to be an effective human T cell antigen, combined with the Th1-driving adjuvant CpG. This novel Th1-inducing vaccine conferred significant protection during S. aureus infection in mice. This study notably advances our understanding of S. aureus cellular immunity, and demonstrates for the first time that a correlate of S. aureus protective immunity identified in mice may be relevant in humans.

  19. Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection.

    Science.gov (United States)

    Brown, Aisling F; Murphy, Alison G; Lalor, Stephen J; Leech, John M; O'Keeffe, Kate M; Mac Aogáin, Micheál; O'Halloran, Dara P; Lacey, Keenan A; Tavakol, Mehri; Hearnden, Claire H; Fitzgerald-Hughes, Deirdre; Humphreys, Hilary; Fennell, Jérôme P; van Wamel, Willem J; Foster, Timothy J; Geoghegan, Joan A; Lavelle, Ed C; Rogers, Thomas R; McLoughlin, Rachel M

    2015-01-01

    Mechanisms of protective immunity to Staphylococcus aureus infection in humans remain elusive. While the importance of cellular immunity has been shown in mice, T cell responses in humans have not been characterised. Using a murine model of recurrent S. aureus peritonitis, we demonstrated that prior exposure to S. aureus enhanced IFNγ responses upon subsequent infection, while adoptive transfer of S. aureus antigen-specific Th1 cells was protective in naïve mice. Translating these findings, we found that S. aureus antigen-specific Th1 cells were also significantly expanded during human S. aureus bloodstream infection (BSI). These Th1 cells were CD45RO+, indicative of a memory phenotype. Thus, exposure to S. aureus induces memory Th1 cells in mice and humans, identifying Th1 cells as potential S. aureus vaccine targets. Consequently, we developed a model vaccine comprising staphylococcal clumping factor A, which we demonstrate to be an effective human T cell antigen, combined with the Th1-driving adjuvant CpG. This novel Th1-inducing vaccine conferred significant protection during S. aureus infection in mice. This study notably advances our understanding of S. aureus cellular immunity, and demonstrates for the first time that a correlate of S. aureus protective immunity identified in mice may be relevant in humans. PMID:26539822

  20. Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection.

    LENUS (Irish Health Repository)

    Brown, Aisling F

    2015-01-01

    Mechanisms of protective immunity to Staphylococcus aureus infection in humans remain elusive. While the importance of cellular immunity has been shown in mice, T cell responses in humans have not been characterised. Using a murine model of recurrent S. aureus peritonitis, we demonstrated that prior exposure to S. aureus enhanced IFNγ responses upon subsequent infection, while adoptive transfer of S. aureus antigen-specific Th1 cells was protective in naïve mice. Translating these findings, we found that S. aureus antigen-specific Th1 cells were also significantly expanded during human S. aureus bloodstream infection (BSI). These Th1 cells were CD45RO+, indicative of a memory phenotype. Thus, exposure to S. aureus induces memory Th1 cells in mice and humans, identifying Th1 cells as potential S. aureus vaccine targets. Consequently, we developed a model vaccine comprising staphylococcal clumping factor A, which we demonstrate to be an effective human T cell antigen, combined with the Th1-driving adjuvant CpG. This novel Th1-inducing vaccine conferred significant protection during S. aureus infection in mice. This study notably advances our understanding of S. aureus cellular immunity, and demonstrates for the first time that a correlate of S. aureus protective immunity identified in mice may be relevant in humans.

  1. Inhibiting platelets aggregation could aggravate the acute infection caused by Staphylococcus aureus.

    Science.gov (United States)

    Zhang, Xin; Liu, Yu; Gao, Yaping; Dong, Jie; Mu, Chunhua; Lu, Qiang; Shao, Ningsheng; Yang, Guang

    2011-01-01

    Several fibrinogen binding proteins (Fibs) play important roles in the pathogenesis of Staphylococcus aureus (S. aureus). Most Fibs can promote the aggregation of platelets during infection, but the extracellular fibrinogen-binding protein (Efb) is an exception. It is reported that Efb can specifically bind fibrinogen and inhibit the aggregation of platelet with its N terminal. However, the biological significance of platelet aggregation inhibition in the infection caused by S. aureus is unclear until now. Here, we demonstrated that the persistence and aggregation of platelets were important for killing S. aureus in whole blood. It was found that the N terminal of Efb (EfbN) and platelets inhibitors could increase the survival of S. aureus in whole blood. The study in vivo also showed that EfbN and platelets inhibitors could reduce the killing of S. aureus and increase the lethality rate of S. aureus in the acute infection mouse model.

  2. Antibiotic-mediated selection of quorum-sensing-negative Staphylococcus aureus

    DEFF Research Database (Denmark)

    Paulander, Wilhelm Erik Axel; Varming, Anders Nissen; Bæk, Kristoffer Torbjørn;

    2012-01-01

    -acquired S. aureus infections and suggest that the adaptability of S. aureus to antibiotics involves the agr locus. IMPORTANCE: Staphylococcus aureus is the most frequently isolated pathogen in intensive care units and a common cause of nosocomial infections, resulting in a high degree of morbidity......Staphylococcus aureus is a human commensal that at times turns into a serious bacterial pathogen causing life-threatening infections. For the delicate control of virulence, S. aureus employs the agr quorum-sensing system that, via the intracellular effector molecule RNAIII, regulates virulence gene...... increases the agr-mediated fitness cost by inducing the expression of RNAIII. Thus, the extensive use of antibiotics in hospitals may explain why agr-negative variants are frequently isolated from hospital-acquired S. aureus infections but rarely found among community-acquired S. aureus strains. Importantly...

  3. Detection of Staphylococcus aureus in Milk Using Real-time Fluorescence Loop-mediated Isothermal Amplification

    Directory of Open Access Journals (Sweden)

    Ying Yu

    2015-07-01

    Full Text Available Staphylococcus aureus is a kind of worldwide food-borne pathogen. Recently, S. aureus has gained considerable attention because of the increasing alimentary toxicosis incidence. In this study, a Real-Time fluorescence Loop-Mediated isothermal Amplification (RT-LAMP was developed to detect S. aureus rapidly. The heat-stable nuclease (nuc gene of S. aureus, the target sequence, was selected to design four special primers. A rapid detection method for S. aureus was initially established under optimum reaction conditions. The assay, performed for 40 min at 61°C, did not show cross reactivity with other bacterial species. The specificity and sensitivity of RT-LAMP for detecting S. aureus were 100% and 8.0 CFU/mL, respectively. Results indicated that RT-LAMP was a potential field-usable molecular tool for detecting S. aureus This method can be an alternative to conventional LAMP in clinical applications and operational programs.

  4. Shedding of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus from adult and pediatric bathers in marine waters

    Directory of Open Access Journals (Sweden)

    Sinigalliano Christopher D

    2011-01-01

    Full Text Available Abstract Background Staphylococcus aureus including methicillin resistant S. aureus, MRSA, are human colonizing bacteria that commonly cause opportunistic infections primarily involving the skin in otherwise healthy individuals. These infections have been linked to close contact and sharing of common facilities such as locker rooms, schools and prisons Waterborne exposure and transmission routes have not been traditionally associated with S. aureus infections. Coastal marine waters and beaches used for recreation are potential locations for the combination of high numbers of people with close contact and therefore could contribute to the exposure to and infection by these organisms. The primary aim of this study was to evaluate the amount and characteristics of the shedding of methicillin sensitive S. aureus, MSSA and MRSA by human bathers in marine waters. Results Nasal cultures were collected from bathers, and water samples were collected from two sets of pools designed to isolate and quantify MSSA and MRSA shed by adults and toddlers during exposure to marine water. A combination of selective growth media and biochemical and polymerase chain reaction analysis was used to identify and perform limited characterization of the S. aureus isolated from the water and the participants. Twelve of 15 MRSA isolates collected from the water had identical genetic characteristics as the organisms isolated from the participants exposed to that water while the remaining 3 MRSA were without matching nasal isolates from participants. The amount of S. aureus shed per person corresponded to 105 to 106 CFU per person per 15-minute bathing period, with 15 to 20% of this quantity testing positive for MRSA. Conclusions This is the first report of a comparison of human colonizing organisms with bacteria from human exposed marine water attempting to confirm that participants shed their own colonizing MSSA and MRSA into their bathing milieu. These findings clearly

  5. Echocardiographic findings predict in-hospital and 1-year mortality in left-sided native valve Staphylococcus aureus endocarditis

    DEFF Research Database (Denmark)

    Lauridsen, Trine K.; Park, Lawrence; Tong, Steven Y C;

    2015-01-01

    BACKGROUND: Staphylococcus aureus left-sided native valve infective endocarditis (LNVIE) has higher complication and mortality rates compared with endocarditis from other pathogens. Whether echocardiographic variables can predict prognosis in S aureus LNVIE is unknown. METHODS AND RESULTS......: Consecutive patients with LNVIE, enrolled between January 2000 and September 2006, in the International Collaboration on Endocarditis were identified. Subjects without S aureus IE were matched to those with S aureus IE by the propensity of having S aureus. Survival differences were determined using log...

  6. Community-onset Staphylococcus aureus Surveillance Programme annual report, 2012.

    Science.gov (United States)

    Coombs, Geoffrey W; Daly, Denise A; Pearson, Julie C; Nimmo, Graeme R; Collignon, Peter J; McLaws, Mary-Louise; Robinson, James O; Turnidge, John D

    2014-03-01

    In 2012, the Australian Group on Antimicrobial Resistance (AGAR) conducted a community-onset period-prevalence survey of clinical Staphylococcus aureus isolated from hospital outpatients and general practice patients including nursing homes, long term care facilities and hospice patients. Day surgery and dialysis patients were excluded. Twenty-nine medical microbiology laboratories from all state and mainland territories participated. Isolates were tested by Vitek2® (AST-P612 card). Results were compared with previous AGAR community surveys. Nationally, the proportion of S. aureus that were methicillin-resistant S. aureus (MRSA) increased significantly from 11.5% in 2000 to 17.9% in 2012 (P<0.0001). Resistance to the non-ß-lactam antimicrobials varied between regions. No resistance was detected to vancomycin, teicoplanin or linezolid. Resistance in methicillin susceptible S. aureus was rare apart from erythromycin (12.8%) and was absent for vancomycin, teicoplanin, linezolid and daptomycin. The proportion of S. aureus characterised as health care-associated MRSA (HA-MRSA) was 5.1%. Three HA-MRSA clones were characterised, with 72.9% and 26.4% of HA-MRSA classified as ST22-IV [2B] (EMRSA-15) and ST239-III [3A] (Aus-2/3 EMRSA) respectively. Multi-clonal community-associated MRSA (CA-MRSA) accounted for 12.5% of all S. aureus. Regional variation in resistance in MRSA was primarily due to the differential distribution of the 2 major HA-MRSA clones; ST239-III [3A] (Aus-2/3 EMRSA), which is resistant to multiple non-ß-lactam antimicrobials, and ST22-IV [2B] (EMRSA-15), which is resistant to ciprofloxacin and typically erythromycin. Although the majority of CA-MRSA were non-multi-resistant, a significant expansion of Panton-Valentine leukocidin (PVL) positive CA-MRSA clones has occurred nationally. The mean age of patients (31.7 years, 95% CI 28.9-34.5) with a PVL positive CA-MRSA infection was significantly lower (P<0.0001), than the mean age of patients with a PVL

  7. Population Genomics of Reduced Vancomycin Susceptibility in Staphylococcus aureus.

    Science.gov (United States)

    Rishishwar, Lavanya; Kraft, Colleen S; Jordan, I King

    2016-01-01

    The increased prevalence of vancomycin-intermediate Staphylococcus aureus (VISA) is an emerging health care threat. Genome-based comparative methods hold great promise to uncover the genetic basis of the VISA phenotype, which remains obscure. S. aureus isolates were collected from a single individual that presented with recurrent staphylococcal bacteremia at three time points, and the isolates showed successively reduced levels of vancomycin susceptibility. A population genomic approach was taken to compare patient S. aureus isolates with decreasing vancomycin susceptibility across the three time points. To do this, patient isolates were sequenced to high coverage (~500×), and sequence reads were used to model site-specific allelic variation within and between isolate populations. Population genetic methods were then applied to evaluate the overall levels of variation across the three time points and to identify individual variants that show anomalous levels of allelic change between populations. A successive reduction in the overall levels of population genomic variation was observed across the three time points, consistent with a population bottleneck resulting from antibiotic treatment. Despite this overall reduction in variation, a number of individual mutations were swept to high frequency in the VISA population. These mutations were implicated as potentially involved in the VISA phenotype and interrogated with respect to their functional roles. This approach allowed us to identify a number of mutations previously implicated in VISA along with allelic changes within a novel class of genes, encoding LPXTG motif-containing cell-wall-anchoring proteins, which shed light on a novel mechanistic aspect of vancomycin resistance. IMPORTANCE The emergence and spread of antibiotic resistance among bacterial pathogens are two of the gravest threats to public health facing the world today. We report the development and application of a novel population genomic

  8. 再担保体系内银保风险共担研究--基于系统动力学%The Study of Bank-guarantee Risk Sharing in Re-guarantee System---Based on System Dynamics

    Institute of Scientific and Technical Information of China (English)

    梅强; 许红珍

    2014-01-01

    In recent years, the development of credit re-guarantee system for Medium-Sized Enterprises (SME) has made great contribution for SME finance in China. As loan risk diversification institution , guarantee and re-guarantee institutions are always at weak positions when they are cooperating with banks. The re-guarantee institutions in China are trying to commit to improve the cooperation mechanism of bank-guarantee, strengthening communication and achieving bank-guarantee risk sharing. The re-guarantee system of risk sharing in some foreign countries is relatively mature and worthy to learn. The re-guarantee inst-itutions, guarantee institutions and cooperative banks have been studied, and the dynamic simulated model of credit re-guarantee system for SME has been constructed. Re-guarantee data from Jiangsu Province have been calculated in the simulated model. The important influences of bank-guarantee risk sharing to the benefits from credit re-guarantee system model have been researched. The results show that banks assuming a certain risk in the process of cooperating with the guarantee institutions can resist the negative effects in development of re-guarantee system with the increasing of compensatory rate. The certain extent of cooperation ensures the sustainable development of re-guarantee system in order to ensure banks to get more long-term interests.%近年来,我国中小企业信用再担保体系的发展为中小企业融资做出了巨大贡献,但作为贷款风险分散机构,担保机构、再担保机构在与银行合作过程中一直处于弱势地位。我国再担保机构一直致力于完善银保合作机制,加强银保沟通,实现银保风险共担。国外一些再担保体系在银保风险共担方面相对比较成熟,值得我国再担保体系学习和借鉴。本文以再担保机构、担保机构和合作银行为研究对象,运用系统动力学,建立中小企业信用再担保体系动态仿真模型。将江

  9. Antimicrobial potential of Pakistani medicinal plants against multi-drug resistance Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Rahat Ejaz

    2014-09-01

    Full Text Available Objective: To determine resistance patterns of Staphylococcus aureus (S. aureus isolated from different areas of Pakistan and to identify antimicrobial agents against multi-drug resistant S. aureus strains. Methods: A total of 67 samples (sewerage, nasal and milk were collected from different farm areas of Pakistan to identify local strains of S. aureus. Sixteen out of 67 samples were positive for S. aureus. Only 6 out of 16 S. aureus strains showed resistance to antibiotics. Then the antibacterial effect of 29 medicinal plants was evaluated on these S. aureus isolates and a standard S. aureus strain ATCC 25923. The solvents used for the extraction of plants were acetone, dimethyl sulfoxide and methanol. The in vitro antibacterial activity was performed using agar disc diffusion method. Moreover, minimum inhibitory concentration of effective medicinal plant extracts was identified through micro-dilution method to find out their 50% inhibitory concentration. Results: Plant extracts of 5 medicinal plants (Psidium guajava, Nigella sativa, Piper nigrum, Valeriana jatamansi, and Cucurbita pepo exhibited antibacterial activity against locally isolated multidrug resistant strains of S. aureus. The minimum inhibitory concentration of these extracts was ranged from 0.328 to 5.000 mg/mL. Conclusions: Plant extracts of Psidium guajava, Piper nigrum seed, Valeriana jatamansi, Cucurbita pepo and Nigella sativa showed significant in vitro antibacterial activity and thus, such findings may serve as valuable contribution in the treatment of infection and may contribute to the development of potential antimicrobial agents against multi drug resistant strains of S. aureus

  10. Antimicrobial potential of Pakistani medicinal plants against multi-drug resistance Staphylococcus aureus

    Institute of Scientific and Technical Information of China (English)

    Rahat Ejaz; Usman A Ashfaq; Sobia Idrees

    2014-01-01

    Objective: To determine resistance patterns of Staphylococcus aureus (S. aureus) isolated from different areas of Pakistan and to identify antimicrobial agents against multi-drug resistant S.aureus strains. Methods: A total of 67 samples (sewerage, nasal and milk) were collected from different farm areas of Pakistan to identify local strains of S. aureus. Sixteen out of 67 samples were positive for S.aureus. Only 6 out of 16 S. aureus strains showed resistance to antibiotics. Then the antibacterial effect of 29 medicinal plants was evaluated on these S. aureus isolates and a standard S. aureus strain ATCC 25923. The solvents used for the extraction of plants were acetone, dimethyl sulfoxide and methanol. The in vitro antibacterial activity was performed using agar disc diffusion method. Moreover, minimum inhibitory concentration of effective medicinal plant extracts was identified through micro-dilution method to find out their 50% inhibitory concentration.Results:Plant extracts of 5 medicinal plants (Psidium guajava, Nigella sativa, Piper nigrum, Valeriana jatamansi, and Cucurbita pepo) exhibited antibacterial activity against locally isolated multidrug resistant strains of S. aureus. The minimum inhibitory concentration of these extracts was ranged from 0.328 to 5.000 mg/mL. Conclusions: Plant extracts of Psidium guajava, Piper nigrum seed, Valeriana jatamansi, Cucurbita pepo and Nigella sativa showed significant in vitro antibacterial activity and thus, such findings may serve as valuable contribution in the treatment of infection and may contribute to the development of potential antimicrobial agents against multi drug resistant strains of S. aureus.

  11. Prevalence and resistance of commensal Staphylococcus aureus, including meticillin-resistant S aureus, in nine European countries: a cross-sectional study.

    NARCIS (Netherlands)

    Heijer, C.D.J. den; Bijnen, E.M.E. van; Paget, W.J.; Pringle, M.; Goossen, H.; Bruggeman, C.A.; Schellevis, F.G.; Stobberingh, E.E.

    2013-01-01

    Background: Information about the prevalence of Staphylococcus aureus resistance to antimicrobial drugs has mainly been obtained from invasive strains, although the commensal microbiota is thought to be an important reservoir of resistance. We aimed to compare the prevalence of nasal S aureus carria

  12. Prevalence and resistance of commensal Staphylococcus aureus, including meticillin-resistant S aureus, in nine European countries: a cross-sectional study

    NARCIS (Netherlands)

    Heijer, C.D. den; Bijnen, E.M. van; Paget, W.J.; Pringle, M.; Goossens, H.; Bruggeman, C.A.; Schellevis, F.G.; Stobberingh, E.E.

    2013-01-01

    BACKGROUND: Information about the prevalence of Staphylococcus aureus resistance to antimicrobial drugs has mainly been obtained from invasive strains, although the commensal microbiota is thought to be an important reservoir of resistance. We aimed to compare the prevalence of nasal S aureus carria

  13. Mastitis Bovina: Resistencia a antibióticos de cepas de Staphylococcus aureus asiladas de leche (Bovine Mastitis: Antimicrobial resistance of Staphylococcus aureus strains isolated from milk

    Directory of Open Access Journals (Sweden)

    Pellegrino, MS

    2011-07-01

    Full Text Available ResumenLa mastitis bovina es considerada la enfermedad infecciosa del ganado lechero de mayor impacto económico mundial, siendo Staphylococcus aureus el principal agente patógeno en muchos países.SummaryBovine mastitis is a frequent cause of economic loss in worldwide dairy herds, being Staphylococcus aureus the main etiological agent in many countries.

  14. Mastitis Bovina: Resistencia a antibióticos de cepas de Staphylococcus aureus asiladas de leche (Bovine Mastitis: Antimicrobial resistance of Staphylococcus aureus strains isolated from milk)

    OpenAIRE

    Pellegrino, MS; Frola, ID; Odierno, LM; Bogni, CI

    2011-01-01

    ResumenLa mastitis bovina es considerada la enfermedad infecciosa del ganado lechero de mayor impacto económico mundial, siendo Staphylococcus aureus el principal agente patógeno en muchos países.SummaryBovine mastitis is a frequent cause of economic loss in worldwide dairy herds, being Staphylococcus aureus the main etiological agent in many countries.

  15. An Aromatic Hydroxyamide Attenuates Multiresistant Staphylococcus aureus Toxin Expression.

    Science.gov (United States)

    Vomacka, Jan; Korotkov, Vadim S; Bauer, Bianca; Weinandy, Franziska; Kunzmann, Martin H; Krysiak, Joanna; Baron, Oliver; Böttcher, Thomas; Lorenz-Baath, Katrin; Sieber, Stephan A

    2016-01-26

    Methicillin-resistant Staphylococcus aureus (MRSA) causes severe infections with only few effective antibiotic therapies currently available. To approach this challenge, chemical entities with a novel and resistance-free mode of action are desperately needed. Here, we introduce a new hydroxyamide compound that effectively reduces the expression of devastating toxins in various S. aureus and MRSA strains. The molecular mechanism was investigated by transcriptome analysis as well as by affinity-based protein profiling. Down-regulation of several pathogenesis associated genes suggested the inhibition of a central virulence-related pathway. Mass spectrometry-based chemical proteomics revealed putative molecular targets. Systemic treatment with the hydroxyamide showed significant reduction of abscess sizes in a MRSA mouse skin infection model. The absence of resistance development in vitro further underlines the finding that targeting virulence could lead to prolonged therapeutic options in comparison to antibiotics that directly address bacterial survival.

  16. Cavity Forming Pneumonia Due to Staphylococcus aureus Following Dengue Fever.

    Science.gov (United States)

    Miyata, Nobuyuki; Yoshimura, Yukihiro; Tachikawa, Natsuo; Amano, Yuichiro; Sakamoto, Yohei; Kosuge, Youko

    2015-11-01

    While visiting Malaysia, a 22-year-old previously healthy Japanese man developed myalgia, headache, and fever, leading to a diagnosis of classical dengue fever. After improvement and returning to Japan after a five day hospitalization, he developed productive cough several days after defervescing from dengue. Computed tomography (CT) thorax scan showed multiple lung cavities. A sputum smear revealed leukocytes with phagocytized gram-positive cocci in clusters, and grew an isolate Staphylococcus aureus sensitive to semi-synthetic penicillin; he was treated successfully with ceftriaxone and cephalexin. This second reported case of pneumonia due to S. aureus occurring after dengue fever, was associated both with nosocomial exposure and might have been associated with dengue-associated immunosuppression. Clinicians should pay systematic attention to bacterial pneumonia following dengue fever to establish whether such a connection is causally associated. PMID:26304914

  17. An Aromatic Hydroxyamide Attenuates Multiresistant Staphylococcus aureus Toxin Expression.

    Science.gov (United States)

    Vomacka, Jan; Korotkov, Vadim S; Bauer, Bianca; Weinandy, Franziska; Kunzmann, Martin H; Krysiak, Joanna; Baron, Oliver; Böttcher, Thomas; Lorenz-Baath, Katrin; Sieber, Stephan A

    2016-01-26

    Methicillin-resistant Staphylococcus aureus (MRSA) causes severe infections with only few effective antibiotic therapies currently available. To approach this challenge, chemical entities with a novel and resistance-free mode of action are desperately needed. Here, we introduce a new hydroxyamide compound that effectively reduces the expression of devastating toxins in various S. aureus and MRSA strains. The molecular mechanism was investigated by transcriptome analysis as well as by affinity-based protein profiling. Down-regulation of several pathogenesis associated genes suggested the inhibition of a central virulence-related pathway. Mass spectrometry-based chemical proteomics revealed putative molecular targets. Systemic treatment with the hydroxyamide showed significant reduction of abscess sizes in a MRSA mouse skin infection model. The absence of resistance development in vitro further underlines the finding that targeting virulence could lead to prolonged therapeutic options in comparison to antibiotics that directly address bacterial survival. PMID:26748534

  18. A Simple Method for Guaranteeing ECG Quality in Real-Time Wavelet Lossy Coding

    Directory of Open Access Journals (Sweden)

    José García

    2007-01-01

    Full Text Available Guaranteeing ECG signal quality in wavelet lossy compression methods is essential for clinical acceptability of reconstructed signals. In this paper, we present a simple and efficient method for guaranteeing reconstruction quality measured using the new distortion index wavelet weighted PRD (WWPRD, which reflects in a more accurate way the real clinical distortion of the compressed signal. The method is based on the wavelet transform and its subsequent coding using the set partitioning in hierarchical trees (SPIHT algorithm. By thresholding the WWPRD in the wavelet transform domain, a very precise reconstruction error can be achieved thus enabling to obtain clinically useful reconstructed signals. Because of its computational efficiency, the method is suitable to work in a real-time operation, thus being very useful for real-time telecardiology systems. The method is extensively tested using two different ECG databases. Results led to an excellent conclusion: the method controls the quality in a very accurate way not only in mean value but also with a low-standard deviation. The effects of ECG baseline wandering as well as noise in compression are also discussed. Baseline wandering provokes negative effects when using WWPRD index to guarantee quality because this index is normalized by the signal energy. Therefore, it is better to remove it before compression. On the other hand, noise causes an increase in signal energy provoking an artificial increase of the coded signal bit rate. Clinical validation by cardiologists showed that a WWPRD value of 10% preserves the signal quality and thus they recommend this value to be used in the compression system.

  19. A Simple Method for Guaranteeing ECG Quality in Real-Time Wavelet Lossy Coding

    Directory of Open Access Journals (Sweden)

    Alesanco Álvaro

    2007-01-01

    Full Text Available Guaranteeing ECG signal quality in wavelet lossy compression methods is essential for clinical acceptability of reconstructed signals. In this paper, we present a simple and efficient method for guaranteeing reconstruction quality measured using the new distortion index wavelet weighted PRD (WWPRD, which reflects in a more accurate way the real clinical distortion of the compressed signal. The method is based on the wavelet transform and its subsequent coding using the set partitioning in hierarchical trees (SPIHT algorithm. By thresholding the WWPRD in the wavelet transform domain, a very precise reconstruction error can be achieved thus enabling to obtain clinically useful reconstructed signals. Because of its computational efficiency, the method is suitable to work in a real-time operation, thus being very useful for real-time telecardiology systems. The method is extensively tested using two different ECG databases. Results led to an excellent conclusion: the method controls the quality in a very accurate way not only in mean value but also with a low-standard deviation. The effects of ECG baseline wandering as well as noise in compression are also discussed. Baseline wandering provokes negative effects when using WWPRD index to guarantee quality because this index is normalized by the signal energy. Therefore, it is better to remove it before compression. On the other hand, noise causes an increase in signal energy provoking an artificial increase of the coded signal bit rate. Clinical validation by cardiologists showed that a WWPRD value of 10 preserves the signal quality and thus they recommend this value to be used in the compression system.

  20. A Simple Method for Guaranteeing ECG Quality in Real-Time Wavelet Lossy Coding

    Science.gov (United States)

    Alesanco, Álvaro; García, José

    2007-12-01

    Guaranteeing ECG signal quality in wavelet lossy compression methods is essential for clinical acceptability of reconstructed signals. In this paper, we present a simple and efficient method for guaranteeing reconstruction quality measured using the new distortion index wavelet weighted PRD (WWPRD), which reflects in a more accurate way the real clinical distortion of the compressed signal. The method is based on the wavelet transform and its subsequent coding using the set partitioning in hierarchical trees (SPIHT) algorithm. By thresholding the WWPRD in the wavelet transform domain, a very precise reconstruction error can be achieved thus enabling to obtain clinically useful reconstructed signals. Because of its computational efficiency, the method is suitable to work in a real-time operation, thus being very useful for real-time telecardiology systems. The method is extensively tested using two different ECG databases. Results led to an excellent conclusion: the method controls the quality in a very accurate way not only in mean value but also with a low-standard deviation. The effects of ECG baseline wandering as well as noise in compression are also discussed. Baseline wandering provokes negative effects when using WWPRD index to guarantee quality because this index is normalized by the signal energy. Therefore, it is better to remove it before compression. On the other hand, noise causes an increase in signal energy provoking an artificial increase of the coded signal bit rate. Clinical validation by cardiologists showed that a WWPRD value of 10[InlineEquation not available: see fulltext.] preserves the signal quality and thus they recommend this value to be used in the compression system.

  1. Alternative Labeling Programs and Purchasing Behavior toward Organic Foods: The Case of the Participatory Guarantee Systems in Brazil

    Directory of Open Access Journals (Sweden)

    Giovanna Sacchi

    2015-06-01

    Full Text Available Regulatory standards and certification models are essential tools guaranteeing the authenticity of organic products. In particular, third-party certification is useful to consumers since it provides guarantees regarding production processes and food quality. In an attempt to cope with the costs and bureaucratic procedures related to the adoption of such certification, groups of small producers have begun to rely upon alternative quality assurance systems such as Participatory Guarantee Systems (PGS. This study contextualizes and analyzes the PGS scheme and describes the Brazilian Rede Ecovida de Agroecologia network. We then investigate the effect of various factors on Brazilian consumers’ purchasing behavior for organic products guaranteed by PGS. The results show that employed and older consumers who live in rural and suburban areas are more likely to buy organic PGS products.

  2. Energy efficient k-anycast routing in multi-sink wireless networks with guaranteed delivery

    OpenAIRE

    Mitton, Nathalie; Simplot-Ryl, David; Voge, Marie-Emilie; Zhang, Lei

    2012-01-01

    International audience In k-anycasting, a sensor wants to report event information to any k sinks in the network. This is important to gain in reliability and efficiency in wireless sensor and actor networks. In this paper, we describe KanGuRou, the first position-based energy efficient k-anycast routing which guarantees the packet delivery to k sinks as long as the connected component that contains s also contains sufficient number of sinks. A node s running KanGuRou first computes a tree...

  3. Guaranteed Delivery in k-Anycast Routing in Multi-SinkWireless Networks

    OpenAIRE

    Nathalie Mitton; David Simplot-Ryl; Jun Zheng

    2013-01-01

    In k-anycasting, a sensor wants to report event information to any k sinks in the network. In this paper, we describe KanGuRou, the first position-based energy efficient k-anycast routing which guarantees the packet delivery to k sinks as long as the connected component that contains s also contains at least k sinks. A node s running KanGuRou first computes a tree including k sinks with weight as low as possible. If this tree has m ≥ 1 edges originated at node s, s duplicates the message m ti...

  4. Guaranteed Delivery in k-Anycast Routing in Multi-Sink Wireless Networks

    OpenAIRE

    Mitton, Nathalie; Simplot-Ryl, David; Zheng, Jun

    2013-01-01

    International audience In k-anycasting, a sensor wants to report event information to any k sinks in the network. In this paper, we describe KanGuRou, the rst position-based energy e cient k-anycast routing which guarantees the packet delivery to k sinks as long as the connected component that contains s also contains at least k sinks. A node s running KanGuRou rst computes a tree including k sinks with weight as low as possible. If this tree has m 1 edges originated at node s, s duplicate...

  5. Optimal guaranteed cost filtering for Markovian jump discrete-time systems

    Directory of Open Access Journals (Sweden)

    Mahmoud Magdi S.

    2004-01-01

    Full Text Available This paper develops a result on the design of robust steady-state estimator for a class of uncertain discrete-time systems with Markovian jump parameters. This result extends the steady-state Kalman filter to the case of norm-bounded time-varying uncertainties in the state and measurement equations as well as jumping parameters. We derive a linear state estimator such that the estimation-error covariance is guaranteed to lie within a certain bound for all admissible uncertainties. The solution is given in terms of a family of linear matrix inequalities (LMIs. A numerical example is included to illustrate the theory.

  6. Are Low-Price Compromises Collusion Guarantees? An Experimental Test of Price Matching Policies

    OpenAIRE

    Enrique Fatás; Juan A. Mañez

    2004-01-01

    In a symmetric differentiated experimental duopoly we test the ability of Price Matching Guarantees (PMG) to rise prices above the competitive levels. PMG is introduced both as a market institution (the effective selling price is always the lowest posted price) and as a strategic choice so subjects have to decide whether or not to offer it. Our results show that PMG leads to a clear collusive outcome as markets quickly and fully converge to the collusive prediction if PM is imposed as a marke...

  7. Massive M2M Access with Reliability Guarantees in LTE Systems

    DEFF Research Database (Denmark)

    Madueño, Germán Corrales; Kiilerich Pratas, Nuno; Stefanovic, Cedomir;

    2015-01-01

    Machine-to-Machine (M2M) communications are one of the major drivers of the cellular network evolution towards 5G systems. One of the key challenges is on how to provide reliability guarantees to each accessing device in a situation in which there is a massive number of almost-simultaneous arrivals...... approach, based on a standard operation of the cellular access. The access procedure is divided into two phases, an estimation phase and a serving phase. In the estimation phase the number of arrivals is estimated and this information is used to tune the amount of resources allocated in the serving phase...

  8. INTERNATIONAL JURISDICTION IN INTELLECTUAL PROPERTY MATTERS: IS THE RIGHT OF ACCESS TO JUSTICE GUARANTEED?

    Directory of Open Access Journals (Sweden)

    Laura Bastos Carvalho

    2015-12-01

    Full Text Available This paper aims to provide an overview of international jurisdiction rules when it comes to intellectual property. Moreover, it provides an analysis of whether access to justice is guaranteed in international conflicts related to intellectual property. Firstly, the concept of international jurisdiction and its related principles are defined. Secondly, an explanation of the intellectual property rights is done in order to introduce the analysis of how international instruments regulate these conflicts. International and national case law are also analyzed. Lastly, it is concluded that legal uncertainty in this field can be itself an obstacle to access to justice.

  9. Major risks and financial guarantees provided by the State in France

    International Nuclear Information System (INIS)

    France's system for indemnifying damage from natural catastrophe is exemplary, whether for floods, storms, or subsidence. However, France is not equipped with the financial capacity to deal with the damage resulting from an exceptional disaster, such as an earthquake on the Mediterranean coast, or a nuclear meltdown. Major catastrophes could be a significant risk to the financial stability of the State today, because the State is in fact the ultimate insurer of its citizens and its institutions. It would be wise to built up reserves in order to enhance the financial resources of the State and to provide a uniform guarantee covering major risks, whatever the cause of the damage may be. (author)

  10. Schistosoma spindale infection in a captive jackal (Canis aureus)

    OpenAIRE

    Vimalraj, P. G.; Latchumikanthan, A.

    2013-01-01

    This report is based on the findings from a captive jackal (Canis aureus) housed in Amirthi Zoological Park, Javadu Hills, Vellore. The animal was reported to be dull, depressed and also had diarrhea. Fecal samples were collected in 10 % formalin and subjected to direct and sedimentation method of faecal examination and was examined for endoparasitic infection. Surprisingly, fecal examination revealed two spindle shaped eggs having terminal spine with a size of 250μ by 60μ. The eggs were iden...

  11. Acral lick dermatitis in a jackal (Canis aureus).

    Science.gov (United States)

    Yeruham, I; Nyska, A

    1998-06-01

    Acral lick dermatitis was diagnosed in a 6-mo-old female jackal (Canis aureus) that was born and housed in a zoological garden in Hafez-Haim, Israel. Other dermatologic diseases were ruled out. Although the lesions were presumed to be psychogenic in origin, they resolved with topical therapy using an ointment containing benzocaine, neomycin sulfate, and hydrocortisone acetate. No recurrence has been observed. PMID:9732044

  12. Applying Convergent Immunity to Innovative Vaccines Targeting Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Michael R Yeaman

    2014-09-01

    Full Text Available Recent perspectives forecast a new paradigm for future 3rd generation vaccines based on commonalities found in diverse pathogens or convergent immune defenses to such pathogens. For Staphylococcus aureus, recurring infections and a limited success of vaccines containing S. aureus antigens imply that native antigens induce immune responses insufficient for optimal efficacy. These perspectives exemplify the need to apply novel vaccine strategies to high priority pathogens. One such approach can be termed convergent immunity, where antigens from non-target organisms that contain epitope homologues found in the target organism are applied in vaccines. This approach aims to evoke atypical immune defenses via synergistic processes that 1 afford protective efficacy; 2 target an epitope from one organism that contributes to protective immunity against another; 3 cross-protect against multiple pathogens occupying a common anatomic or immunologic niche; and/or 4 overcome immune subversion or avoidance strategies of target pathogens. Thus, convergent immunity has a potential to promote protective efficacy not usually elicited by native antigens from a target pathogen. Variations of this concept have been mainstays in the history of viral and bacterial vaccine development. A more far-reaching example is the pre–clinical evidence that specific fungal antigens can induce cross-kingdom protection against bacterial pathogens. This trans-kingdom protection has been demonstrated in preclinical studies of the recombinant Candida albicans agglutinin-like sequence 3 protein (rAls3 where it was shown that a vaccine containing rAls3 provides homologous protection against C. albicans, heterologous protection against several other Candida species, and convergent protection against several strains of S. aureus. Convergent immunity reflects an intriguing new approach to designing and developing vaccine antigens and is considered here in the context of vaccines to target

  13. Fatal pneumoni med Panton-Valentine-leukocidinproducerende Staphylococcus aureus

    DEFF Research Database (Denmark)

    Rabøl, Peter Hedelund; Dessau, Ram Benny; Warnecke, Mads;

    2010-01-01

    We describe a case of fatal pneumonia in a previously healthy 14-year-old boy. The patient was severely affected at the time of admission with high fever, tachypnea, tachycardia and peripheral cyanosis. The condition worsened despite treatment with antibiotics as well as respiratory and pressure ...... support. Acidosis and critical leucopenia supervened and the patient died just short of 24 hours after admission. Subsequent bacterial cultivation showed Panton-Valentine Leucocidin-producing Staphylococcus aureus....

  14. Colostrum Hexasaccharide, a Novel Staphylococcus aureus Quorum-Sensing Inhibitor

    OpenAIRE

    Srivastava, A; Singh, B.N.; Deepak, D.; Rawat, A. K. S.; Singh, B. R.

    2015-01-01

    The discovery of quorum-sensing (QS) systems regulating antibiotic resistance and virulence factors (VFs) has afforded a novel opportunity to prevent bacterial pathogenicity. Dietary molecules have been demonstrated to attenuate QS circuits of bacteria. But, to our knowledge, no study exploring the potential of colostrum hexasaccharide (CHS) in regulating QS systems has been published. In this study, we analyzed CHS for inhibiting QS signaling in Staphylococcus aureus. We isolated and charact...

  15. Quinupristin/dalfopristin in Staphylococcus aureus endophthalmitis: a case report

    OpenAIRE

    Hernandez-Da Mota Sergio E

    2011-01-01

    Abstract Introduction The intravitreal injection of antibiotics remains the mainstay of therapy for postoperative endophthalmitis. Bacterial resistance, however, is still a pitfall in achieving an adequate response to treatment. Quinupristin/dalfopristin might be a feasible therapeutic option in these cases. Case presentation A 55-year-old Hispanic man had endophthalmitis secondary to Staphylococcus aureus in his right eye and was treated with intravitreal 0.4 mg/0.1 ml quinupristin/dalfopris...

  16. Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridization.

    OpenAIRE

    Dickgiesser, N; Kreiswirth, B N

    1986-01-01

    A method is described for identification of the genes conferring aminoglycoside resistance in Staphylococcus aureus by dot-blot and Southern blot techniques. As radioactive probes, fragments of plasmids pAT48, pUBH2, and pH13, carrying the genes for an aminocyclitol-3'-phosphotransferase, an aminocyclitol-4'-adenylyltransferase, and an aminocyclitol-2''-phosphotransferase-aminocyclitol-6'-acetyltransferase, respectively, were used.

  17. Personal Hygiene and Methicillin-resistant Staphylococcus aureus Infection

    OpenAIRE

    Turabelidze, George; Lin, Mei; Wolkoff, Barbara; Dodson, Douglas; Gladbach, Stephen; Zhu, Bao-Ping

    2006-01-01

    Methicillin-resistant Staphylococcus aureus (MRSA) infections outside the healthcare setting are an increasing concern. We conducted a case-control study to investigate an MRSA outbreak during 2002–2003 in a Missouri prison and focused on hygiene factors. Information on sociodemographic characteristics, medical history, and hygiene practices of study participants was collected by interview and medical record review. Logistic regression was used to evaluate MRSA infection in relation to hygien...

  18. Global distribution and diversity of ovine-associated Staphylococcus aureus.

    Science.gov (United States)

    Smith, Edward M; Needs, Polly F; Manley, Grace; Green, Laura E

    2014-03-01

    Staphylococcus aureus is an important pathogen of many species, including sheep, and impacts on both human and animal health, animal welfare, and farm productivity. Here we present the widest global diversity study of ovine-associated S. aureus to date. We analysed 97 S. aureus isolates from sheep and sheep products from the UK, Turkey, France, Norway, Australia, Canada and the USA using multilocus sequence typing (MLST) and spa typing. These were compared with 196 sheep isolates from Europe (n=153), Africa (n=28), South America (n=14) and Australia (n=1); 172 bovine, 68 caprine and 433 human S. aureus profiles. Overall there were 59 STs and 87 spa types in the 293 ovine isolates; in the 97 new ovine isolates there were 22 STs and 37 spa types, including three novel MLST alleles, four novel STs and eight novel spa types. Three main CCs (CC133, CC522 and CC700) were detected in sheep and these contained 61% of all isolates. Four spa types (t002, t1534, t2678 and t3576) contained 31% of all isolates and were associated with CC5, CC522, CC133 and CC522 respectively. spa types were consistent with MLST CCs, only one spa type (t1403) was present in multiple CCs. The three main ovine CCs have different but overlapping patterns of geographical dissemination that appear to match the location and timing of sheep domestication and selection for meat and wool production. CC133, CC522 and CC700 remained ovine-associated following the inclusion of additional host species. Ovine isolates clustered separately from human and bovine isolates and those from sheep cheeses, but closely with caprine isolates. As with cattle isolates, patterns of clonal diversification of sheep isolates differ from humans, indicative of their relatively recent host-jump.

  19. Bactericidal antibiotic-phytochemical combinations against methicillin resistant Staphylococcus aureus

    OpenAIRE

    Bhone Myint Kyaw; Shuchi arora; Chu Sing Lim

    2012-01-01

    Methicillin resistant Staphylococcus aureus (MRSA) infection is a global concern nowadays. Due to its multi-drug resistant nature, treatment with conventional antibiotics does not assure desired clinical outcomes. Therefore, there is a need to find new compounds and/or alternative methods to get arsenal against the pathogen. Combination therapies using conventional antibiotics and phytochemicals fulfill both requirements. In this study, the efficacy of different phytochemicals in combination ...

  20. Nanoadhesion of Staphylococcus aureus onto Titanium Implant Surfaces

    OpenAIRE

    Aguayo, S.; Donos, N.; Spratt, D.; Bozec, L.

    2015-01-01

    Adhesion of bacteria to dental implant surfaces is the critical initial step in the process of biofilm colonization; however, the specific nanoadhesive interactions occurring during the first contact between bacterial cells and biomaterial substrates remain poorly understood. In this report, we utilize single-cell force spectroscopy to characterize the dynamics of the initial interaction between living Staphylococcus aureus cells and machined titanium surfaces at the nanoscale. Values for max...

  1. Coated vesicle isolation by immunoadsorption on Staphylococcus aureus cells

    OpenAIRE

    1982-01-01

    Porcine brain coated vesicles were isolated from crude fractions of tissue homogenates by affinity separation using anticlathrin-coated STaphylococcus aureus (Staph A) cells as a solid-phase immunoadsorbent. The specificity of the immunoadsorption was monitored by SDS PAGE analysis and by competitive ELISA assays. SDS PAGE of the material immunoadsorbed from a fraction of porcine bran smooth microsomes showed a selective enrichment in a 180,000 mol wt protein. In an ELISA assay, this protein ...

  2. Analysis of Cell Wall Teichoic Acids in Staphylococcus aureus.

    Science.gov (United States)

    Covas, Gonçalo; Vaz, Filipa; Henriques, Gabriela; Pinho, Mariana G; Filipe, Sérgio R

    2016-01-01

    Most bacterial cells are surrounded by a surface composed mainly of peptidoglycan (PGN), a glycopolymer responsible for ensuring the bacterial shape and a telltale molecule that betrays the presence of bacteria to the host immune system. In Staphylococcus aureus, as in most gram-positive bacteria, peptidoglycan is concealed by covalently linked molecules of wall teichoic acids (WTA)-phosphate rich molecules made of glycerol and ribitol phosphates which may be tailored by different amino acids and sugars.In order to analyze and compare the composition of WTA produced by different S. aureus strains, we describe methods to: (1) quantify the total amount of WTA present at the bacterial cell surface, through the determination of the inorganic phosphate present in phosphodiester linkages of WTA; (2) identify which sugar constituents are present in the assembled WTA molecules, by detecting the monosaccharides, released by acid hydrolysis, through an high-performance anion exchange chromatography analysis coupled with pulsed amperometric detection (HPAEC-PAD) and (3) compare the polymerization degree of WTA found at the cell surface of different S. aureus strains, through their different migration in a polyacrylamide gel electrophoresis (PAGE). PMID:27311674

  3. Nanoscale Plasma Coating Inhibits Formation of Staphylococcus aureus Biofilm.

    Science.gov (United States)

    Xu, Yuanxi; Jones, John E; Yu, Haiqing; Yu, Qingsong; Christensen, Gordon D; Chen, Meng; Sun, Hongmin

    2015-12-01

    Staphylococcus aureus commonly infects medical implants or devices, with devastating consequences for the patient. The infection begins with bacterial attachment to the device, followed by bacterial multiplication over the surface of the device, generating an adherent sheet of bacteria known as a biofilm. Biofilms resist antimicrobial therapy and promote persistent infection, making management difficult to futile. Infections might be prevented by engineering the surface of the device to discourage bacterial attachment and multiplication; however, progress in this area has been limited. We have developed a novel nanoscale plasma coating technology to inhibit the formation of Staphylococcus aureus biofilms. We used monomeric trimethylsilane (TMS) and oxygen to coat the surfaces of silicone rubber, a material often used in the fabrication of implantable medical devices. By quantitative and qualitative analysis, the TMS/O2 coating significantly decreased the in vitro formation of S. aureus biofilms; it also significantly decreased in vivo biofilm formation in a mouse model of foreign-body infection. Further analysis demonstrated TMS/O2 coating significantly changed the protein adsorption, which could lead to reduced bacterial adhesion and biofilm formation. These results suggest that TMS/O2 coating can be used to effectively prevent medical implant-related infections.

  4. Biochemical characters and antibiotic susceptibility of Staphylococcus aureus isolates

    Institute of Scientific and Technical Information of China (English)

    Subhankari Prasad Chakraborty; Santanu Kar Mahapatra; Somenath Roy

    2011-01-01

    Objective: To observe the biochemical characters and antibiotic susceptibility of isolated Staphylococcus aureus (S. auerus) strains against some conventional and traditional antibiotics.Methods:Bacterial culture was done in Mueller-Hinton broth at 37 ℃. Characters of these strains were determined by traditional biochemical tests such as hydrolysis test of gelatin, urea, galactose, starch and protein, and fermentation of lactose and sucrose. Antibiotic susceptibility were carried out by minimum inhibitory concentration test, minium bactericidal concentration test, disc agar diffusion test and brain heart infusion oxacillin screening agar. Results: From this study, it was observed that 100% S. aureus isolates showed positive results in gelatin, urea and galactose hydrolysis test, 50% isolates were positive in starch hydrolysis test, 35% in protein hydrolysis test, 100% isolates in lactose fermenting test, but no isolate was positive in sucrose fermenting test. Antibiotic susceptibility testing suggested that 20% of isolates were resistant to kanamycin and 46.67% were resistant to oxacillin. Conclusions: These findings show that all these isolates have gelatin, urea, galactose hydrolysis and lactose fermenting activity. 20% of these isolates were resistant to kanamycin and 46.67% were resistant to oxacillin. Thirty post operative pathogenic isolated S. aureus strains were used in this study.

  5. Synthetic peptide inhibitors of DNA replication in Staphylococcus aureus

    DEFF Research Database (Denmark)

    Løbner-Olesen, Anders; Kjelstrup, Susanne

    of clinically important pathogens and is essential for bacterial proliferation. The bacterial replication apparatus fulfill the requirements for a good drug target. The replisome of S. aureus consists of 5 different subunits (2, PolC2, 4, δ and δ`) who’s organization depends on multiple protein......-protein interactions. Centrally in the replisome is the -clamp where to multiple proteins binds through a conserved motif. We have identified the protein-protein interactions in the replisome of S. aureus by use of a bacterial two-hybrid system. A reverse bacterial two-hybrid system (R-BTH) based on Pyr......N (), DnaB and DnaX (). Three peptides identified as inhibitors of DnaN have been purified. Two of these peptides inhibited growth as well as DNA replication in S. aureus. The minimal inhibitory concentration (MIC) of the peptides was approximately 50 g/ml. Overexpression of DnaN reduced the inhibitory...

  6. Novel antibiotics for the treatment of Staphylococcus aureus.

    Science.gov (United States)

    Ohlsen, Knut

    2009-11-01

    Staphylococcus aureus is a leading cause of nosocomial and community-acquired infection associated with significant morbidity and mortality. Antibiotic treatment of infections owing to S. aureus have become increasingly challenging as the pathogen has acquired a broad spectrum of antibiotic resistance mechanisms. In particular, emergence and spread of methicillin-resistant S. aureus (MRSA) progressed to a global health threat. The glycopeptides antibiotics vancomycin and teicoplanin have remained as the drugs of last resort for more than 20 years. Fortunately, in addition to the glycopeptides, several novel antibiotics including linezolid, daptomycin, tigecycline, quinupristin/dalfopristin and ceftobiprole acting against MRSA have been recently introduced into clinical practice broadening therapeutic options. Although the arsenal of antistaphylococcal drugs has filled up in recent years, the rate of MRSA infection continues to be high in most countries. This demands an ongoing search for new antibacterials and lead compounds as well as development of alternative therapies and faster diagnostics to ensure effective anti-staphylococcal therapy in the future. PMID:22112259

  7. Staphylococcus aureus Regulatory RNAs as Potential Biomarkers for Bloodstream Infections.

    Science.gov (United States)

    Bordeau, Valérie; Cady, Anne; Revest, Matthieu; Rostan, Octavie; Sassi, Mohamed; Tattevin, Pierre; Donnio, Pierre-Yves; Felden, Brice

    2016-09-01

    Staphylococcus aureus is a commensal bacterium and pathogen. Identifying biomarkers for the transition from colonization to disease caused by this organism would be useful. Several S. aureus small RNAs (sRNAs) regulate virulence. We investigated presence and expression of 8 sRNAs in 83 S. aureus strains from 42 patients with sepsis or septic shock and 41 asymptomatic colonized carriers. Small pathogenicity island sRNAs sprB and sprC were clade specific. Six sRNAs had variable expression not correlated with clinical status. Expression of RNAIII was lower in strains from septic shock patients than in strains from colonized patients. When RNAIII was associated with expression of sprD, colonizing strains could be discriminated from strains in patients with bloodstream infections, including patients with sepsis and septic shock. Isolates associated with colonization might have sRNAs with target expression different from those of disease isolates. Monitoring expression of RNAIII and sprD could help determine severity of bloodstream infections. PMID:27224202

  8. Modulation of Drug Resistance in Staphylococcus aureus with Coumarin Derivatives

    Directory of Open Access Journals (Sweden)

    Rodrigo Santos Aquino de Araújo

    2016-01-01

    Full Text Available Semisynthetic and commercial coumarins were investigated for their antibacterial and adjuvant properties with antibiotic agents against norfloxacin, erythromycin, and tetracycline resistant Staphylococcus aureus as based on efflux mechanisms. The coumarins and certain commercial antibiotics had their Minimum Inhibitory Concentrations determined by broth microdilution assay against resistant S. aureus strains which overexpress efflux pump proteins. For evaluation of the modulatory activity, the antibiotics MICs were determined in the presence of the coumarin derivatives at subinhibitory concentration. Although the coumarins did not display relevant antibacterial activity (MIC ≥ 128 µg/mL, they did modulate the antibiotics activities. Various coumarins, especially the alkylated derivatives in combination with antibiotics at subinhibitory concentrations, modulated antibiotic activity, reducing the MIC for tetracycline and norfloxacin by 2 to 8 times. Polar Surface Area (PSA studies were performed and the fact that the presence of apolar groups is an important factor for the modulatory activity of coumarins was corroborated. Docking on the Penicillin-Binding Protein from MRSA identified that 18 is a potential ligand presenting low Ebinding. The results indicate that coumarin derivatives modulated antibiotic resistance and may be used as potential antibiotic adjuvants, acting by bacterial efflux pump inhibition in S. aureus.

  9. Predictors of Staphylococcus aureus Colonization and Results after Decolonization

    Directory of Open Access Journals (Sweden)

    Tennison L. Malcolm

    2016-01-01

    Full Text Available Protocols for the screening and decolonization of Staphylococcus aureus prior to total joint arthroplasty (TJA have become widely adopted. The goals of this study were to determine: (1 whether implementation of a screening protocol followed by decolonization with mupirocin/vancomycin and chlorhexidine reduces the risk of revision compared with no screening protocol (i.e., chlorhexidine alone and (2 whether clinical criteria could reliably predict colonization with MSSA and/or MRSA. Electronic medical records of primary patients undergoing TJA that were screened (n=3,927 and were not screened (n=1,751 for Staphylococcus aureus at least 4 days prior to surgery, respectively, were retrospectively reviewed. All patients received chlorhexidine body wipes preoperatively. Patients carrying MSSA and MRSA were treated preoperatively with mupirocin and vancomycin, respectively, along with the standard preoperative antibiotics and chlorhexidine body wipes. Screened patients were 50% less likely to require revision due to prosthetic joint infection compared to those not screened (p=0.04. Multivariate regression models were poorly accurate in predicting colonization with MSSA (AUC = 0.58 and MRSA (AUC = 0.62. These results support the routine screening and decolonization of S. aureus prior to TJA.

  10. Pricing of the Cross-Currency Interest Rate Guarantee Embedded in Financial Contracts in a LIBOR Market Model

    Directory of Open Access Journals (Sweden)

    Tsung-Yu Hsieh

    2015-12-01

    Full Text Available We derive the pricing formulae for the financial contracts, such as guaranteed investment contracts (GICs, life insurance contracts, pension plans, and others, with the guaranteed minimum rate of return set relative to a LIBOR interest rate. Further, we analyze the guaranteed contracts in which the asset that provides the underlying return for the contract and the guaranteed interest rate are denominated in different currencies, which is a common practice. The guaranteed contracts with the above characteristics are called “cross-currency interest rate guaranteed contracts” (CIRGCs. Valuation of such contracts has not been investigated in the previous literature. To value CIRGCs, a cross-currency LIBOR market model is introduced. The LIBOR market model for a single-currency economy is extended to a cross-currency economy which incorporates the traded-asset prices and exchange rate processes into the model setting. The cross-currency LIBOR market model (CLMM is suitable and applicable to pricing a variety of CIRGCs. The pricing formulas derived under the CLMM are more tractable and feasible for practice than those derived under the instantaneous short rate model or the HJM model. Four different types of CIRGCs are priced in this article. Calibration procedures are also discussed for practical implementation. In addition, Monte-Carlo simulation is provided to evaluate the accuracy of the theoretical prices.

  11. The growth of Staphylococcus aureus and Escherichia coli in low-direct current electric fields

    Institute of Scientific and Technical Information of China (English)

    Dunya Zituni; Heidi Schu tt-Gerowitt; Marion Kopp; Martin Kro nke; Klaus Addicks; Christian Hoffmann; Martin Hellmich; Franz Faber; Wilhelm Niedermeier

    2014-01-01

    Electrical potentials up to 800 mV can be observed between different metallic dental restorations. These potentials produce fields in the mouth that may interfere with microbial communities. The present study focuses on the impact of different electric field strengths (EFS) on the growth of Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922) in vitro. Cultures of S. aureus and E. coli in fluid and gel medium were exposed to different EFS. Effects were determined by calculation of viable counts and measurement of inhibition zones. In gel medium, anodic inhibition zones for S. aureus were larger than those for E. coli at all field strength levels. In fluid medium, the maximum decrease in the viable count of S. aureus cells was at 10 V?m21. Field-treated S. aureus cells presented ruptured cell walls and disintegrated cytoplasm. Conclusively, S. aureus is more sensitive to increasing electric field strength than E. coli.

  12. Dispersal of Bap-mediated Staphylococcus aureus biofilm by proteinase K.

    Science.gov (United States)

    Kumar Shukla, Sudhir; Rao, Toleti Subba

    2013-02-01

    The dominant role of biofilm-associated protein (Bap) in Staphylococcus aureus biofilm development prompted us to investigate Bap as a potential target for proteinase-mediated biofilm dispersion. Biofilm assay in microtitre plates showed that proteinase K hampered the early adhesion of cells as well as biofilm development. Proteinase K treatment of 24- and 48-h-old biofilms showed enhanced dispersion of bap-positive S. aureus biofilm; however, proteinase K did not affect the bap-negative S. aureus biofilm. When antibiotics were used in combination with proteinase K, significant enhancement in antibiotic action was noticed against bap-positive S. aureus biofilm. This study establishes that antibiotics in combination with proteinase K can be used for controlling S. aureus biofilms in whose development Bap surface protein has a major role. We propose that Bap protein could be a potential target for therapeutic control of S. aureus infections (for example, bovine mastitis).

  13. Use of mupirocin-chlorhexidine treatment to prevent Staphylococcus aureus surgical-site infections.

    Science.gov (United States)

    Bertrand, X; Slekovec, C; Talon, D

    2010-05-01

    Evaluation of: Bode LGM, Kluytmans JAJW, Wertheim HFL et al.: Preventing surgical-site infections in nasal carriers of Staphylococcus aureus. N. Engl. J. Med. 362, 9-17 (2010). Staphylococcus aureus is the main pathogen responsible for surgical-site infections and nasal carriage is a major risk factor for subsequent infection with this bacteria. Mupirocin is considered to be the topical antibacterial agent of choice for eradication of nasal S. aureus. The paper by Bode et al. provides strong evidence that the combination of a rapid identification of a S. aureus nasal carrier, mupirocin nasal ointment and chlorhexidine gluconate soap, significantly reduces the rate of S. aureus surgical-site infection by nearly 60%. In conclusion, mupirocin nasal ointment use in S. aureus carriers before surgery has numerous advantages with few side effects. PMID:20441543

  14. One-year mortality in coagulase-negative Staphylococcus and Staphylococcus aureus infective endocarditis

    DEFF Research Database (Denmark)

    Rasmussen, Rasmus V; Snygg-Martin, Ulrika; Olaison, Lars;

    2009-01-01

    The aim of this study was to investigate in-hospital mortality and 12-month mortality in patients with coagulase-negative Staphylococcus (CoNS) compared to Staphylococcus aureus (S. aureus) infective endocarditis (IE). We used a prospective cohort study of 66 consecutive CoNS and 170 S. aureus IE...... patients, collected at 2 tertiary university hospitals in Copenhagen (Denmark) and at 1 tertiary university hospital in Gothenburg (Sweden). Median (range) C-reactive protein at admission was higher in patients with S. aureus IE (150 mg/l (1-521) vs 94 mg/l (6-303); p...% of patients with S. aureus IE (p =0.05). In conclusion, CoNS IE was associated with a long diagnostic delay and high in-hospital mortality, whereas post-discharge prognosis was better in this group of patients compared to patients with IE due to S. aureus....

  15. Be alert to the alterations in the biological characteristics in heterogeneous vancomycin-intermediate Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    X Zhou

    2012-01-01

    Full Text Available The development of reduced vancomycin susceptibility in Staphylococcus aureus in many cases appears to be associated with characteristic changes. These changes may have pitfall of identifying S. aureus by automated testing methods like Vitek 32. In this study, we retested 24 heterogeneous vancomycin-intermediate Staphylococcus haemolyticus (h-VISH collected in 2008-2010 at the Department of Clinical Microbiology by conventional biochemical tests and polymerase chain reaction (PCR. The heterogeneous vancomycin-intermediate S. aureus (hVISA reversion test and electron microscopic examination were also used. Six isolates of 24 h-VISH possessed nuc, coa, and 16S rRNA genes, and could be reversed into S. aureus. It suggested that biochemical and morphological changes in hVISA and vancomycin-intermediate S. aureus (VISA should be considered, and the detection of S. aureus, especially reduced vancomycin susceptibility isolates, requires more attention and different techniques.

  16. Staphylococcus aureus, thermostable nuclease and staphylococcal enterotoxins in raw ewes' milk Manchego cheese.

    Science.gov (United States)

    Nuñez, M; Bautista, L; Medina, M; Gaya, P

    1988-07-01

    Growth and survival of two enterotoxigenic strains of Staphylococcus aureus were studied during manufacture and ripening of eight batches of raw ewes' milk Manchego cheese. Only 2-3 generations of Staph. aureus occurred in the vat and during pressing. The death rate of Staph. aureus (mean decrease in log cfu/g/week of ripening) from day 1 to day 60 was 0.421 in cheese made with 1% Streptococcus lactis starter and 0.404 in cheese made without starter. Thermostable nuclease was produced in the vat by growing Staph. aureus cells; it was inactivated by rennet during the first 24 h and synthesized again by surviving cells of Staph. aureus from day 1 to day 60. Staphylococcal enterotoxins A, B, C and D were not detected in any batches of cheese, even though Staph. aureus counts exceeded 10(7) cfu/g. PMID:3209513

  17. The role of staphylothrombin-mediated fibrin deposition in catheter-related Staphylococcus aureus infections.

    Science.gov (United States)

    Vanassche, Thomas; Peetermans, Marijke; Van Aelst, Lucas N L; Peetermans, Willy E; Verhaegen, Jan; Missiakas, Dominique M; Schneewind, Olaf; Hoylaerts, Marc F; Verhamme, Peter

    2013-07-01

    Staphylococcus aureus (S. aureus) is a frequent cause of catheter-related infections. S. aureus secretes the coagulases staphylocoagulase and von Willebrand factor-binding protein, both of which form a staphylothrombin complex upon binding to prothrombin. Although fibrinogen and fibrin facilitate the adhesion of S. aureus to catheters, the contribution of staphylothrombin-mediated fibrin has not been examined. In this study, we use a S. aureus mutant lacking both coagulases (Δcoa/vwb) and dabigatran, a pharmacological inhibitor of both staphylothrombin and thrombin, to address this question. Genetic absence or chemical inhibition of pathogen-driven coagulation reduced both fibrin deposition and the retention of S. aureus on catheters in vitro. In a mouse model of jugular vein catheter infection, dabigatran reduced bacterial load on jugular vein catheters, as well as metastatic kidney infection. Importantly, inhibition of staphylothrombin improved the efficacy of vancomycin treatment both in vitro and in the mouse model. PMID:23532100

  18. Role of GapC in the pathogenesis of Staphylococcus aureus.

    Science.gov (United States)

    Kerro-Dego, Oudessa; Prysliak, Tracy; Perez-Casal, Jose; Potter, Andrew A

    2012-05-01

    Staphylococcus aureus is recognized worldwide as a major pathogen causing clinical or subclinical intramammary infections in lactating cows, sheep and goats. S. aureus produces a wide arsenal of cell surface and extracellular proteins involved in virulence. Among these are two conserved proteins with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity named glyceraldehyde-3-phosphate dehydrogenase-B (GapB) and -C (GapC). In this study, we used the S. aureus wild type strain RN6390 and its isogenic gapC mutant H330 in in vitro and in vivo studies and determined that the S. aureus GapC protein plays a role on adherence to and internalization into bovine mammary epithelial (MAC-T) cells. In addition, we found that S. aureus H330 did not caused mastitis after an experimental infection of ovine mammary glands. Together, these results show that GapC is important in the pathogenesis of S. aureus mastitis. PMID:22176759

  19. Pattern differentiation in co-culture biofilms formed by Staphylococcus aureus and Pseudomonas aeruginosa

    DEFF Research Database (Denmark)

    Yang, Liang; Liu, Yang; Markussen, Trine;

    2011-01-01

    important for understanding of biofilm physiology and the treatment of biofilm-related infectious diseases. Here, we have investigated interactions of two of the major bacterial species of cystic fibrosis lung microbial communities -Pseudomonas aeruginosa and Staphylococcus aureus- when grown in co......-culture biofilms. By growing co-culture biofilms of S. aureus with P. aeruginosa mutants in a flow-chamber system and observing them using confocal laser scanning microscopy, we show that wild-type P. aeruginosa PAO1 facilitates S. aureus microcolony formation. In contrast, P. aeruginosa mucA and rpoN mutants do...... not facilitate S. aureus microcolony formation and tend to outcompete S. aureus in co-culture biofilms. Further investigations reveal that extracellular DNA (eDNA) plays an important role in S. aureus microcolony formation and that P. aeruginosa type IV pili are required for this process, probably through...

  20. Molecular Characterization of Staphylococcus aureus Isolates Transmitted between Patients with Buruli Ulcer.

    Directory of Open Access Journals (Sweden)

    Nana Ama Amissah

    Full Text Available Buruli ulcer (BU is a skin infection caused by Mycobacterium ulcerans. The wounds of most BU patients are colonized with different microorganisms, including Staphylococcus aureus.This study investigated possible patient-to-patient transmission events of S. aureus during wound care in a health care center. S. aureus isolates from different BU patients with overlapping visits to the clinic were whole-genome sequenced and analyzed by a gene-by-gene approach using SeqSphere(+ software. In addition, sequence data were screened for the presence of genes that conferred antibiotic resistance.SeqSphere(+ analysis of whole-genome sequence data confirmed transmission of methicillin resistant S. aureus (MRSA and methicillin susceptible S. aureus among patients that took place during wound care. Interestingly, our sequence data show that the investigated MRSA isolates carry a novel allele of the fexB gene conferring chloramphenicol resistance, which had thus far not been observed in S. aureus.

  1. Antimicrobial susceptibility of Staphylococcus aureus and characterization of methicillin-resistant Staphylococcus aureus isolated from bovine mastitis in Korea.

    Science.gov (United States)

    Nam, Hyang-Mi; Lee, Ae-Li; Jung, Suk-Chan; Kim, Mal-Nam; Jang, Geum-Chan; Wee, Sung-Hwan; Lim, Suk-Kyung

    2011-02-01

    A total of 402 Staphylococcus aureus isolates from bovine mastitis milk collected during 2003-2009 in Korea were tested for susceptibility to 20 antimicrobial agents. All S. aureus isolates were susceptible to 11 of 20 antimicrobials tested; no resistance was observed against pirlimycin, telithromycin, novobiocin, penicillin/novobiocin, quinupristin/dalfopristin, clindamycin, rifampin, ciprofloxacin, trimethprim/sulfamethoxazol, vancomycin, and linezolid. Over 66% of the S. aureus isolates were resistant to penicillin. Resistance was also seen for gentamicin (11.9%), erythromycin (7.7%), methicillin (oxacillin and cefoxitin, 6.2%), and tetracycline (4.2%). No noticeable change was observed in penicillin, gentamicin, and erythromycin resistance over the 7-year period. Tetracycline resistance appeared to decrease consistently, whereas methicillin resistance was observed from 2005. About 2.7% (11/402) were resistant to three or more antimicrobials. Genotyping of the 17 methicillin-resistant S. aureus (MRSA) isolated from each cow revealed two staphylococcal cassette chromosome mec (SCCmec) types (IV and IVa), three spa types (t286, t324, and untypable), and two sequence types (ST1 and ST72). Eleven of 17 (64.7%) MRSA strains belonged to SCCmec IVa-t324-ST72. The rest of strains belonged to SCCmec IVa-t286-ST1 (n = 3) and SCCmec IV-untypable-ST72 (n = 3). None of the MRSA carried the Panton-Valentine leukocidin gene. These characteristics are the same as those found in community-acquired (CA) MRSA strains prevalent in humans in Korea. Three pulsed-field gel electrophoresis types (A-C) were observed among the 17 MRSA strains examined, and 14 strains belonged to the same pulsed-field gel electrophoresis pattern regardless of their geographical origin and year of isolation. The results of this study provide evidence of CA-MRSA infection in dairy cattle for the first time in Korea. PMID:21034263

  2. ROMANIAN DEPOSIT GUARANTEE SCHEME AND THE REQUIREMENTS OF THE ACQUIS COMMUNAUTAIRE

    Directory of Open Access Journals (Sweden)

    Cristian Bichi

    2002-12-01

    Full Text Available This paper compares the Romanian and EU deposit insurance legislation in order to identify the changes, which are needed in order to obtain a full compatibility of the national regulations in the field of deposit protection with the corresponding European rules. The first part outlines the main features of the EU Directive 94/19/EEC on deposit guarantee schemes in order to find out to what extent Romania is bound to incorporate the relevant acquis communautaire into its national legislation. It results that the directive contains explicit harmonization provisions as regards the minimum level of protection (EUR 20,000, depositor co-insurance, types of depositors, instruments and currencies to be covered, nature of coverage, status of branches from EU and non-EU countries, the compensation period and information to depositors. The directive does not provide for the harmonization of the financial and administrative structure of the deposit guarantee schemes, Romania being free to choose from a wider range of options. The second part examines the legal environment of deposit protection in Romania. The third part identifies the measures to be taken by the Romanian authorities in order to fill in the remaining regulatory gaps in the area of deposit insurance. Finally, the fourth part contains the conclusions.

  3. An integrated DBP for streams with (m,k)-firm real-time guarantee

    Institute of Scientific and Technical Information of China (English)

    王智; 陈积明; 孙优贤

    2004-01-01

    (m,k)-firm real-time or weakly hard real-time(WHRT)guarantee is becoming attractive as it closes the gap between hard and soft(or probabilistic)real-time guarantee,and enables finer granularity of real-time QoS through adjusting m and k.For multiple streams with(m,k)-firm constraint sharing a single server,an on-line priority assignment policy based on the most recent k-length history of each stream called distance based priority(DBP)has been proposed to assign priority.In case of priority equality among these head-of-queue instances,Earliest Deadline First(EDF)is used.Under the context of WHRT schedule theory,DBP is the most popular,gets much attention and has many applications due to its straightforward priority assignment policy and easy implementation.However,DBP combined with EDF cannot always provide good performance,mainly because the initial DBP does not underline the rich information on deadline met/missed distribution,specially streams in various failure states which will travel different distances to restore success states.Considering how to effectively restore the success state of each individual stream from a failure state,an integrated DBP utilizing deadline met/missed distribution is proposed in this paper.Simulation results validated the performance improvement of this proposal.

  4. An integrated DBP for streams with (m, k)-firm real-time guarantee

    Institute of Scientific and Technical Information of China (English)

    王智; 陈积明; 孙优贤

    2004-01-01

    (m, k)-firm real-time or weakly hard real-time (WHRT) guarantee is becoming attractive as it closes the gap between hard and soft (or probabilistic) real-time guarantee, and enables finer granularity of real-time QoS through adjusting m and k. For multiple streams with (m, k)-firm constraint sharing a single server, an on-line priority assignment policy based on the most recent k-length history of each stream called distance based priority (DBP) has been proposed to assign priority.In case of priority equality among these head-of-queue instances, Earliest Deadline First (EDF) is used. Under the context of WHRT schedule theory, DBP is the most popular, gets much attention and has many applications due to its straightforward priority assignment policy and easy implementation. However, DBP combined with EDF cannot always provide good performance, mainly because the initial DBP does not underline the rich information on deadline met/missed distribution,specially streams in various failure states which will travel different distances to restore success states. Considering how to effectively restore the success state of each individual stream from a failure state, an integrated DBP utilizing deadline met/missed distribution is proposed in this paper. Simulation results validated the performance improvement of this pro-posal.

  5. Identification of Staphylococcus aureus Colony-Spreading Stimulatory Factors from Mammalian Serum

    OpenAIRE

    Yosuke Omae; Kazuhisa Sekimizu; Chikara Kaito

    2014-01-01

    Staphylococcus aureus forms giant colonies on soft-agar surfaces, which is called colony-spreading. In the present study, we searched for host factors that influence S. aureus colony-spreading activity. The addition of calf serum, porcine serum, or silkworm hemolymph to soft-agar medium stimulated S. aureus colony-spreading activity. Gel filtration column chromatography of calf serum produced a high molecular weight fraction and a low molecular weight fraction, both of which exhibited colony-...

  6. Subinhibitory concentrations of perilla oil affect the expression of secreted virulence factor genes in Staphylococcus aureus.

    Directory of Open Access Journals (Sweden)

    Jiazhang Qiu

    Full Text Available BACKGROUND: The pathogenicity of staphylococcus aureus is dependent largely upon its ability to secrete a number of virulence factors, therefore, anti-virulence strategy to combat S. aureus-mediated infections is now gaining great interest. It is widely recognized that some plant essential oils could affect the production of staphylococcal exotoxins when used at subinhibitory concentrations. Perilla [Perilla frutescens (L. Britton], a natural medicine found in eastern Asia, is primarily used as both a medicinal and culinary herb. Its essential oil (perilla oil has been previously demonstrated to be active against S. aureus. However, there are no data on the influence of perilla oil on the production of S. aureus exotoxins. METHODOLOGY/PRINCIPAL FINDINGS: A broth microdilution method was used to determine the minimum inhibitory concentrations (MICs of perilla oil against S. aureus strains. Hemolysis, tumour necrosis factor (TNF release, Western blot, and real-time RT-PCR assays were performed to evaluate the effects of subinhibitory concentrations of perilla oil on exotoxins production in S. aureus. The data presented here show that perilla oil dose-dependently decreased the production of α-toxin, enterotoxins A and B (the major staphylococcal enterotoxins, and toxic shock syndrome toxin 1 (TSST-1 in both methicillin-sensitive S. aureus (MSSA and methicillin-resistant S. aureus (MRSA. CONCLUSIONS/SIGNIFICANCE: The production of α-toxin, SEA, SEB, and TSST-1 in S. aureus was decreased by perilla oil. These data suggest that perilla oil may be useful for the treatment of S. aureus infections when used in combination with β-lactam antibiotics, which can increase exotoxins production by S. aureus at subinhibitory concentrations. Furthermore, perilla oil could be rationally applied in food systems as a novel food preservative both to inhibit the growth of S. aureus and to repress the production of exotoxins, particularly staphylococcal enterotoxins.

  7. Risk factors for Staphylococcus aureus nasal colonization in Danish middle-aged and elderly twins

    DEFF Research Database (Denmark)

    Andersen, P S; Larsen, Lisbeth Aagaard; Fowler, V G;

    2013-01-01

    Staphylococcus aureus is a human commensal bacterium found in the nasal cavity and other body sites. Identifying risk factors for S. aureus nasal carriage is of interest, as nasal carriage is a risk factor for subsequent invasive infection. We recently investigated the influence of host genetics ......, male gender, psoriasis, and atopic diseases. Also, present living on a farm is clearly associated with S. aureus colonization, while smoking had a borderline statistically significant protective effect....

  8. Infertility as a Consequence of Spermagglutinating Staphylococcus aureus Colonization in Genital Tract of Female Mice

    OpenAIRE

    Siftjit Kaur; Vijay Prabha

    2012-01-01

    Various studies have shown Staphylococcus aureus to be one of the most prevalent organism in male and female genital tract but most practitioners dismiss it as mere contamination which is assumed to be of no significance. However, it is now suggested that the presence of this organism should not be ignored, as incubation of spermatozoa with S. aureus results in reduced sperm motility. Although S. aureus has been reported to cause immobilization of spermatozoa, however, its role in infertility...

  9. High Level Expression and Purification of Atl, the Major Autolytic Protein of Staphylococcus aureus

    OpenAIRE

    Singh, Vineet K.

    2014-01-01

    Staphylococcus aureus is a major human and animal pathogen. Autolysins regulate the growth, turnover, cell lysis, biofilm formation, and the pathogenicity of S. aureus. Atl is the major autolysin in S. aureus. The biochemical and structural studies of staphylococcal Atl have been limited due to difficulty in cloning, high level overexpression, and purification of this protein. This study describes successful cloning, high level over-expression, and purification of two forms of fully functiona...

  10. High Genetic Diversity among Community-Associated Staphylococcus aureus in Europe: Results from a Multicenter Study

    OpenAIRE

    Rolo, Joana; Miragaia, Maria; Turlej-Rogacka, Agata; Empel, Joanna; Bouchami, Ons; Faria, Nuno A.; Tavares, Ana; Hryniewicz, Waleria; Fluit, Ad C.; de Lencastre, Hermínia

    2012-01-01

    Background Several studies have addressed the epidemiology of community-associated Staphylococcus aureus (CA-SA) in Europe; nonetheless, a comprehensive perspective remains unclear. In this study, we aimed to describe the population structure of CA-SA and to shed light on the origin of methicillin-resistant S. aureus (MRSA) in this continent. Methods and Findings A total of 568 colonization and infection isolates, comprising both MRSA and methicillin-susceptible S. aureus (MSSA), were recover...

  11. Differential Expression and Roles of Staphylococcus aureus Virulence Determinants during Colonization and Disease

    OpenAIRE

    Jenkins, Amy; Diep, Binh An; Mai, Thuy T.; Vo, Nhung H.; Warrener, Paul; Suzich, Joann; Stover, C. Kendall; Sellman, Bret R.

    2015-01-01

    ABSTRACT Staphylococcus aureus is a Gram-positive, commensal bacterium known to asymptomatically colonize the human skin, nares, and gastrointestinal tract. Colonized individuals are at increased risk for developing S. aureus infections, which range from mild skin and soft tissue infections to more severe diseases, such as endocarditis, bacteremia, sepsis, and osteomyelitis. Different virulence factors are required for S. aureus to infect different body sites. In this study, virulence gene ex...

  12. Mechanism of hetero-erythromycin resistant Staphylococcus aureus and a comparison of detection methods

    Institute of Scientific and Technical Information of China (English)

    陈东科

    2014-01-01

    Objective To explore the phenotypes and genotypes of Staphylococcus aureus(S.aureus)hetero-resistant to erythromycin and clindamycin and compare their detection methods so as to report results accurately to guide clinical rational use of antibiotics.Methods D test was used to detect the phenotypes of S.aureus hetero-resistant to erythromycin.And then the results of two methods(automated instrument and disk diffusion)were analyzed.All strains were continuously passaged for 50 generations to

  13. Evaluation of high-dose daptomycin for therapy of experimental Staphylococcus aureus foreign body infection

    OpenAIRE

    Lew Daniel P; Bento Manuela; Schaad Heinz J; Vaudaux Pierre

    2006-01-01

    Abstract Background Daptomycin is a novel cyclic lipopeptide whose bactericidal activity is not affected by current antibiotic resistance mechanisms displayed by S. aureus clinical isolates. This study reports the therapeutic activity of high-dose daptomycin compared to standard regimens of oxacillin and vancomycin in a difficult-to-treat, rat tissue cage model of experimental therapy of chronic S. aureus foreign body infection. Methods The methicillin-susceptible S. aureus (MSSA) strain I20 ...

  14. Application of molecular techniques in the study of Staphylococcus aureus clonal evolution - A Review

    Directory of Open Access Journals (Sweden)

    Adriana Marcos Vivoni

    2005-11-01

    Full Text Available Staphylococcus aureus is an important agent of healthcare-associated and community-acquired infections. A major characteristic of this microorganism is the ability to develop resistance to antimicrobial agents. Several molecular techniques have been applied for the characterization of S. aureus in epidemiological studies. In the present review, we discuss the application of molecular techniques for typing S. aureus strains and describe the nomenclature and evolution of epidemic clones of this important pathogen.

  15. Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis

    OpenAIRE

    Le Maréchal Caroline; Jardin Julien; Jan Gwenaël; Even Sergine; Pulido Coralie; Guibert Jean-Michel; Hernandez David; François Patrice; Schrenzel Jacques; Demon Dieter; Meyer Evelyne; Berkova Nadia; Thiéry Richard; Vautor Eric; Le Loir Yves

    2011-01-01

    Abstract Staphylococcus aureus is a major cause of mastitis in ruminants. In ewe mastitis, symptoms range from subclinical to gangrenous mastitis. S. aureus factors or host-factors contributing to the different outcomes are not completely elucidated. In this study, experimental mastitis was induced on primiparous ewes using two S. aureus strains, isolated from gangrenous (strain O11) or subclinical (strain O46) mastitis. Strains induced drastically distinct clinical symptoms when tested in ew...

  16. A rare case of acute epiglottitis due to Staphylococcus aureus in an adult

    Directory of Open Access Journals (Sweden)

    Clare Harris

    2012-01-01

    Full Text Available Epiglottitis has been mainly associated with childhood infection with Haemophilis influenzae type B but cases of adult epiglottitis are increasing. We report here a case of adult epiglottitis and present evidence that it was caused by S. aureus. A 48-year old patient with clinical symptoms of epiglottitis grew Staphylococcus aureus in pure culture from an epiglottal swab. Staphylococcus aureus should be considered as a potential pathogen in adult epiglottitis.

  17. Methicillin-Resistant Staphylococcus aureus Associated with Animals and Its Relevance to Human Health

    OpenAIRE

    AnnalisaPantosti

    2012-01-01

    Staphylococcus aureus is a typical human pathogen. Some animal S. aureus lineages have derived from human strains following profound genetic adaptation determining a change in host specificity. Due to the close relationship of animals with the environmental microbioma and resistoma, animal staphylococcal strains also represent a source of resistance determinants. Methicillin-resistant S. aureus (MRSA) emerged fifty years ago as a nosocomial pathogen but in the last decade it has also become...

  18. A case-control study of mastitis: nasal carriage of Staphylococcus aureus

    OpenAIRE

    Garland Suzanne M; Amir Lisa H; Lumley Judith

    2006-01-01

    Abstract Background Mastitis is a common problem for breastfeeding women. Researchers have called for an investigation into the possible role of maternal nasal carriage of S. aureus in the causation of mastitis in breastfeeding women. Methods The aim of the study was to investigate the role of maternal S. aureus nasal carriage in mastitis. Other factors such as infant nasal S. aureus carriage, nipple damage, maternal fatigue and oversupply of milk were also investigated. A case-control design...

  19. Novel Chromosomally Encoded Multidrug Efflux Transporter MdeA in Staphylococcus aureus

    OpenAIRE

    Huang, Jianzhong; O'Toole, Paul W.; Shen, Wei; Amrine-Madsen, Heather; Jiang, Xinhe; Lobo, Neethan; Palmer, Leslie M.; Voelker, LeRoy; Fan, Frank; Gwynn, Michael N.; McDevitt, Damien

    2004-01-01

    Antibiotic efflux is an important mechanism of resistance in pathogenic bacteria. Here we describe the identification and characterization of a novel chromosomally encoded multidrug resistance efflux protein in Staphylococcus aureus, MdeA (multidrug efflux A). MdeA was identified from screening an S. aureus open reading frame expression library for resistance to antibiotic compounds. When overexpressed, MdeA confers resistance on S. aureus to a range of quaternary ammonium compounds and antib...

  20. Development and Evaluation of a Chromogenic Agar Medium for Methicillin-Resistant Staphylococcus aureus

    OpenAIRE

    Perry, John D.; Davies, Amie; Butterworth, Lynne A.; Hopley, Andrew L. J.; Nicholson, Audrey; Gould, F. Kate

    2004-01-01

    We describe here the development and evaluation of MRSA ID, a new chromogenic agar medium for the specific isolation and identification of methicillin-resistant Staphylococcus aureus (MRSA). We used S. aureus ID (bioMérieux, La Balme Les Grottes, France) and supplemented it with various antimicrobials, including cefoxitin, ciprofloxacin, oxacillin, and methicillin. Cefoxitin proved to be superior to the other antimicrobials for the selection of MRSA from other strains of S. aureus. MRSA ID (c...