WorldWideScience

Sample records for aureus gatcab guarantee

  1. The essential mycobacterial amidotransferase GatCAB is a modulator of specific translational fidelity.

    Science.gov (United States)

    Su, Hong-Wei; Zhu, Jun-Hao; Li, Hao; Cai, Rong-Jun; Ealand, Christopher; Wang, Xun; Chen, Yu-Xiang; Kayani, Masood Ur Rehman; Zhu, Ting F; Moradigaravand, Danesh; Huang, Hairong; Kana, Bavesh D; Javid, Babak

    2016-01-01

    Although regulation of translation fidelity is an essential process(1-7), diverse organisms and organelles have differing requirements of translational accuracy(8-15), and errors in gene translation serve an adaptive function under certain conditions(16-20). Therefore, optimal levels of fidelity may vary according to context. Most bacteria utilize a two-step pathway for the specific synthesis of aminoacylated glutamine and/or asparagine tRNAs, involving the glutamine amidotransferase GatCAB(21-25), but it had not been appreciated that GatCAB may play a role in modulating mistranslation rates. Here, by using a forward genetic screen, we show that the mycobacterial GatCAB enzyme complex mediates the translational fidelity of glutamine and asparagine codons. We identify mutations in gatA that cause partial loss of function in the holoenzyme, with a consequent increase in rates of mistranslation. By monitoring single-cell transcription dynamics, we demonstrate that reduced gatCAB expression leads to increased mistranslation rates, which result in enhanced rifampicin-specific phenotypic resistance. Consistent with this, strains with mutations in gatA from clinical isolates of Mycobacterium tuberculosis show increased mistranslation, with associated antibiotic tolerance, suggesting a role for mistranslation as an adaptive strategy in tuberculosis. Together, our findings demonstrate a potential role for the indirect tRNA aminoacylation pathway in regulating translational fidelity and adaptive mistranslation. PMID:27564922

  2. BANK GUARANTEES

    Directory of Open Access Journals (Sweden)

    VASILE NEMES

    2012-05-01

    Full Text Available The present study propose the analyse of the irrevocable commitment of a bank entity towards a determined person, through which guarantees a certain legal conduct of its client, and, in case of breach, assumes the payment obligation of a determined amount of money. This kind of legal technique it is called bank guarantee and in the usual business language it is called “Letter of Bank Guarantee”. The determined reason to choose this scientific initiative it is the frequency of this kind of financial - banking commitments with various practical issues which are occurred by the use of those. From the legal point of view, the bank guarantees are not under an own legal regulation and are based on the common law, used in the guarantees domain. Through the new aspects of the actual Civil Code it are the legal regulation of the letter of bank guarantee and of the comfort letter, which shall constitute the main regulation in the negotiation and conclusion of a letter of bank guarantee or a bank comfort letter. For the legal reports with foreign elements, the parties can also use the Uniform Rules regarding the Guarantees at Request (URGR Publish no.758/2010.

  3. BANK GUARANTEES

    OpenAIRE

    VASILE NEMES

    2012-01-01

    The present study propose the analyse of the irrevocable commitment of a bank entity towards a determined person, through which guarantees a certain legal conduct of its client, and, in case of breach, assumes the payment obligation of a determined amount of money. This kind of legal technique it is called bank guarantee and in the usual business language it is called “Letter of Bank Guarantee”. The determined reason to choose this scientific initiative it is the frequency of this kind of fin...

  4. The Guarantees of Freedom

    OpenAIRE

    Andrei Shleifer; Rafael La Porta; Florencio Lopez de Silanes; Cristian Pop-Eleches

    2002-01-01

    Hayek (1960) distinguishes the institutions of English freedom, which guarantee the independence of judges from political interference in the administration of justice, from those of American freedom, which allow judges to restrain law-making powers of the sovereign through constitutional review. We create a data base of constitutional rules in 71 countries that reflect these institutions of English and American freedom, and ask whether these rules predict economic and political freedom in a ...

  5. Refund Guarantees: On-demand bond or true guarantee?

    OpenAIRE

    Athukorale, Anjalie Narmadha

    2013-01-01

    In the modern commercial world guarantees have become an important instrument. Guarantees are not limited to those given by a bank in the commercial world. Guarantees may also be issued by a parent company on behalf of a subsidiary, or by an individual(s) in personal transactions. The question then is what is a contract of guarantee? A guarantee is an assurance or security issued by one party (the guarantor ) at the request of the party with the liability (the principal ) to the party the l...

  6. CONSIDERATIONS CONCERNING GUARANTEE SCHEMES

    Directory of Open Access Journals (Sweden)

    EMILIA CLIPICI

    2013-05-01

    Full Text Available When a large withdrawal from banks occurs, customers withdraw their deposits, so banks are likely to go bankrupt because of liquidity problems. There are several mechanisms that allow the banking system to avoid the phenomenon of massive withdrawals from banks. The most effective one is the deposit insurance. The deposit insurance is seen primarily as a means of protecting depositors of credit institutions, and secondly as a means of ensuring the stability of the banking system. This article described deposit guarantee scheme in Romania and other country.

  7. Do job security guarantees work?

    OpenAIRE

    Bryson, Alex; Cappellari, Lorenzo; Lucifora, Claudio

    2004-01-01

    We investigate the effect of employer job security guarantees on employee perceptions of job security. Using linked employer-employee data from the 1998 British Workplace Employee Relations Survey, we find job security guarantees reduce employee perceptions of job insecurity. This finding is robust to endogenous selection of job security guarantees by employers engaging in organisational change and workforce reductions. Furthermore, there is no evidence that increased job security through job...

  8. Guaranteed energy bills for homes

    International Nuclear Information System (INIS)

    Recent developments from the company that established the UK's National Home Energy Rating schemes are reported. The extension into social housing has proved highly popular and an energy cost guarantee which guarantees the main heating and hot water bill of a home will not exceed a pre-specified level, with a cash refund if it does, has been developed for the home occupier market. (UK)

  9. Staphylococcus aureus and Pregnancy

    Science.gov (United States)

    Staphylococcus aureus and Pregnancy In every pregnancy, a woman starts out with a 3-5% chance of ... risk. This sheet talks about whether exposure to staphylococcus aureus may increase the risk for birth defects ...

  10. Staphylococcus aureus toxins

    OpenAIRE

    Otto, Michael

    2013-01-01

    Staphylococcus aureus is a dangerous pathogen that causes a variety of severe diseases. The virulence of S. aureus is defined by a large repertoire of virulence factors, among which secreted toxins play a preeminent role. Many S. aureus toxins damage biological membranes, leading to cell death. In particular, S. aureus produces potent hemolysins and leukotoxins. Among the latter, some were recently identified to lyse neutrophils after ingestion, representing an especially powerful weapon agai...

  11. Infrastructure Franchising and Government Guarantees Infrastructure Franchising and Government Guarantees

    Directory of Open Access Journals (Sweden)

    Alexander Galetovic

    1998-03-01

    Full Text Available Government guarantees for private infrastructure projects reduce the incentives of firms to perform efficiently, weaken the incentives to screen projects for white elephants, and shift government obligations to future periods. Thus the use of guarantees needs to be limited, and they need to be carefully designed. Franchising schemes should in principle assign risks to the parties best able to manage and control them. The mechanisms by which contracts are awarded should be simple, so that possibilities for evaluator subjectivity are reduced, the award process remains as transparent as possible, and the likelihood of having to renegotiate is minimized. Infrastructure franchises have usually been awarded on a fixed-term basis. Such contracts expose franchise holders to considerable demand risk, which investors are often unwilling to assume without government guarantees. These contracts are also inflexible, since it is difficult to determine a fair level of compensation to the franchise holder if the contract is terminated early or modified. Under an alternative mechanism, the franchise is awarded to the firm that asks for the least present value of user fee revenue for a given tariff structure, and the franchise ends when the present value of user fee revenues is equal to the franchise holder's bid. Such contracts reduce the demand risk borne by the franchise holder (and the concomitant demand for government guarantees. They also make fair compensation of franchise holders in the event of early termination straightforward, since the level of fair compensation is equal to the revenue remaining to be collected. Government guarantees for private infrastructure projects reduce the incentives of firms to perform efficiently, weaken the incentives to screen projects for white elephants, and shift government obligations to future periods. Thus the use of guarantees needs to be limited, and they need to be carefully designed. Franchising schemes should in

  12. Equity-Linked Pension Schemes with Guarantees

    OpenAIRE

    J. Aase Nielsen; Klaus Sandmann; Erik Schlogl

    2010-01-01

    This paper analyses the relationship between the level of a return guarantee in an equity-linked pension scheme and the proportion of an investor's contribution needed to finance this guarantee. Three types of schemes are considered: investment guarantee, contribution guarantee and participation surplus. The evaluation of each scheme involves pricing an Asian option, for which relatively tight upper and lower bounds can be calculated in a numerically efficient manner. We find a negative (and ...

  13. Financial guarantee Nuclear Energy Law. Final report

    International Nuclear Information System (INIS)

    In the altered nuclear energy law in the Netherlands it is stated that licensees need to give financial guarantees to cover the cost of closing and dismantling a facility for which the license was granted. Three types of guarantees are distinguished. In this report the the suitability of the different guarantees are described within the framework of the Dutch nuclear energy law. Recommendations are made about the conditions for such guarantees with respect to the design, size, quality and information dissemination

  14. SLA guarantees for cloud services

    OpenAIRE

    Serrano, Damián; Bouchenak, Sara; Kouki, Yousri; Alvares De Oliveira Jr., Frederico; Ledoux, Thomas; Lejeune, Jonathan; Sopena, Julien; Arantes, Luciana; Sens, Pierre

    2016-01-01

    Quality-of-service and SLA guarantees are among the major challenges of cloud-based services. In this paper we first present a new cloud model called SLAaaS — SLA aware Service. SLAaaS considers QoS levels and SLA as first class citizens of cloud-based services. This model is orthogonal to other SaaS, PaaS, and IaaS cloud models, and may apply to any of them. More specifically we make three contributions: (i) we provide a novel domain specific language that allows to describe QoS-oriented SLA...

  15. 78 FR 46528 - Surety Bond Guarantee Program

    Science.gov (United States)

    2013-08-01

    ... definition to the maximum amounts of any Contract or Order when SBA guarantees the bond in connection with a...; ] SMALL BUSINESS ADMINISTRATION 13 CFR Part 115 RIN 3245-AG56 Surety Bond Guarantee Program AGENCY: U.S... regulations governing the Surety Bond Guarantee Program to certain provisions of the National...

  16. Price Guarantees, Consumer Search, and Hassle Costs

    OpenAIRE

    Baake, Pio; Schwalbe, Ulrich

    2013-01-01

    The paper deals with the competitive effects of price guarantees in a spatial duopoly where consumers can search for lower prices but have to incur hassle costs if they want to claim a price guarantee. It is shown that symmetric equilibria with and without price guarantees exist but price guarantees will have no effect on prices if search costs are low, hassle costs are high and the number of uninformed consumers is small. However, when both firms use price guarantees, there also exist payoff...

  17. 7 CFR 1720.14 - Nature of guarantee; acceleration of guaranteed bonds.

    Science.gov (United States)

    2010-01-01

    ... the terms and conditions of the bond documents; (3) The guarantee fee required by 7 CFR 1720.10 of... requirements listed in 7 CFR 1720.12; or (5) The guaranteed lender failed to comply with any material covenant... 7 Agriculture 11 2010-01-01 2010-01-01 false Nature of guarantee; acceleration of guaranteed...

  18. Nuclear accidents - Liabilities and guarantees

    International Nuclear Information System (INIS)

    The 1992 Symposium on Nuclear Accidents - Liabilities and guarantees, organized by the OECD NUCLEAR Energy Agency in collaboration with the international Atomic Energy Agency, discussed the nuclear third party liability regime established by the Paris and Vienna Conventions, its advantages and shortcomings, and assessed the teachings of the Chernobyl accident in the context of that regime. The topics included the geographical scope of the Conventions, the definition of nuclear damage, in particular environmental damage, insurance cover and capacity, supplementary compensation by means of a collective contribution from the nuclear industry or governments, and finally, the international liability of States in case of a nuclear accident. This proceeding contains 26 papers which have been selected

  19. Does Intelligence Always Guarantee Performance?

    Directory of Open Access Journals (Sweden)

    Uzma Hanif Gondal

    2013-04-01

    Full Text Available The current research is intended to explore the individuals’ cognitive intelligence and its impact on their performance. The study examines the intellectual capabilities of employees in reference to the problems that arises due to the interaction with varying job related tasks. Raven’s progressive matrices were used to measure the IQ level of employees. This IQ test was intended to measure the non-verbal intelligence, spatial intelligence, mathematical and logical intelligence of employees. It was hypothesized that individuals’ intelligence does not always guarantee performance. The final data was collected on 300 employees from different organizations of Telecom sector in the city of Lahore, Pakistan. Outcome of the analysis revealed interesting findings that Intelligence Quotient is found to be insignificantly related with individuals’ performance. It shows that IQ alone is not a strong predictor of employees’ performance. Intelligence may ensure a reach to entry level position in an organization but for a sustained position other competencies are of utmost importance. This study may provoke new lines of research in the field of positive psychology specifically.

  20. Staphylococcus aureus Transcriptome Architecture

    DEFF Research Database (Denmark)

    Mäder, Ulrike; Nicolas, Pierre; Depke, Maren;

    2016-01-01

    Staphylococcus aureus is a major pathogen that colonizes about 20% of the human population. Intriguingly, this Gram-positive bacterium can survive and thrive under a wide range of different conditions, both inside and outside the human body. Here, we investigated the transcriptional adaptation of S...... to their dependence on the RNA polymerase sigma factors SigA or SigB, and allow identification of new potential targets for several known transcription factors. In particular, this study revealed a relatively low abundance of antisense RNAs in S. aureus, where they overlap only 6% of the coding genes, and only 19...... antisense RNAs not co-transcribed with other genes were found. Promoter analysis and comparison with Bacillus subtilis links the small number of antisense RNAs to a less profound impact of alternative sigma factors in S. aureus. Furthermore, we revealed that Rho-dependent transcription termination...

  1. Staphylococcus aureus CC398

    DEFF Research Database (Denmark)

    Price, Lance B.; Stegger, Marc; Hasman, Henrik;

    2012-01-01

    Since its discovery in the early 2000s, methicillin-resistant Staphylococcus aureus (MRSA) clonal complex 398 (CC398) has become a rapidly emerging cause of human infections, most often associated with livestock exposure. We applied whole-genome sequence typing to characterize a diverse collection...... of CC398 isolates (n = 89), including MRSA and methicillin-susceptible S. aureus (MSSA) from animals and humans spanning 19 countries and four continents. We identified 4,238 single nucleotide polymorphisms (SNPs) among the 89 core genomes. Minimal homoplasy (consistency index = 0.9591) was detected...

  2. What Guarantees Should Society Offer Its Youth?

    Science.gov (United States)

    Brooks, Chris

    2014-01-01

    Criticising the concept of a "youth guarantee" as it was understood by the OECD in the 1990s and recently again by the European Community, the author argues that just more schooling and training will not help, let alone guarantee a future for disadvantaged, unemployed young people. Rather any policy that wants to help youth to become…

  3. 29 CFR 4022.3 - Guaranteed benefits.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Guaranteed benefits. 4022.3 Section 4022.3 Labor Regulations Relating to Labor (Continued) PENSION BENEFIT GUARANTY CORPORATION COVERAGE AND BENEFITS BENEFITS PAYABLE IN TERMINATED SINGLE-EMPLOYER PLANS General Provisions; Guaranteed Benefits § 4022.3...

  4. 77 FR 38015 - Community Programs Guaranteed Loans

    Science.gov (United States)

    2012-06-26

    ... INFORMATION CONTACT: Kendra Doedderlein, Community Programs Senior Loan Specialist, Rural Housing Service, U.S... (RHS) proposes to amend the regulations utilized to service the Community Facilities Guaranteed Loan... that are eligible for a Community Facilities Guaranteed Loan. The intended effect of this action is...

  5. Marketing service guarantees for health care.

    Science.gov (United States)

    Levy, J S

    1999-01-01

    The author introduces the concept of service guarantees for application in health care and differentiates between explicit, implicit, and conditional vs. unconditional types of guarantees. An example of an unconditional guarantee of satisfaction is provided by the hospitality industry. Firms conveying an implicit guarantee are those with outstanding reputations for products such as luxury automobiles, or ultimate customer service, like Nordstrom. Federal Express and Domino's Pizza offer explicit guarantees of on-time delivery. Taking this concept into efforts to improve health care delivery involves a number of caveats. Customers invited to use exceptional service cards may use these to record either satisfaction or dissatisfaction. The cards need to provide enough specific information about issues so that "immediate action could be taken to improve processes." Front-line employees should be empowered to respond to complaints in a meaningful way to resolve the problem before the client leaves the premises. PMID:10711165

  6. 78 FR 36018 - Bond Guarantee Program; Notice of Guarantee Availability (NOGA) Inviting Qualified Issuer...

    Science.gov (United States)

    2013-06-14

    ... interim rule that governs the CDFI Bond Guarantee Program was published on February 5, 2013 (78 FR 8296... credit subsidy calculation in order to determine the feasibility and risk of the proposed Bond Issue, as... Community Development Financial Institutions Fund Bond Guarantee Program; Notice of Guarantee...

  7. Guarantees in Banking Operations with Bank Customers

    Directory of Open Access Journals (Sweden)

    Vasile Dedu

    2009-07-01

    Full Text Available In a crediting relationship, one of the parties involved – namely the creditor – is exposed to certain risks which impose taking measures to guarantee a debt. If, at the due date, the debtor cannot pay his/her debts and meet the assumed liabilities, the creditor can execute the legally constituted guarantees, on a contract basis, thus covering the debt.There are also situations when the state, being directly interested in promoting trade and cooperation with other economies or simply out of the wish to ensure a competitive and safer business environment, gets involved in insurance and guarantee operations. In such situations, the state does not interve

  8. Green optical networks with connection availability guarantee.

    Science.gov (United States)

    Zhang, Xiaoning; Tan, Shufang

    2015-06-29

    In this paper, we study green optical networks with connection availability guarantee. After introducing a power consumption model for green optical network design, we propose a design method called Availability-Guaranteed Energy-Efficient Design (AGEED). In AGEED, we employ dedicated path protection as backup capacity in order to improve connection availability. Based on iterative routing by modifying link weights, we can make idle network resources be switched into sleep mode, and obtain the energy saving effect. Our simulation results show that AGEED is more advantageous to achieve energy-efficient purpose and guarantee the satisfied connection availability levels in comparison with other methods. PMID:26191755

  9. Financing of large energy projects: The guarantee scheme

    International Nuclear Information System (INIS)

    Financing through export credit: Evolution of the concept (buyer's credit, soft loans, concession, BOT, guarantee scheme). Guarantee scheme in Turkey: Thermic power plant; Hydroelectric power plant; The Energy Fund (characteristics, source of financing of the Funds, guarantees). Conclusion of the presented guarantee scheme: Necessary involvement of all the parts for guarantees and financings. (author)

  10. Understanding consumer response to service guarantees

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, E.L.; Cole, J.M.

    1999-10-01

    Whether the goal is customer loyalty to a competitive energy service provider or customer satisfaction with monopoly distribution services, service guarantees must be seen as one way a company can communicate with its customers about the relationship it has (or wants) with them. In short, service guarantees must be evaluated as part of a company's overall brand management strategy. To do this, a conceptual framework within which to develop and link models of service delivery and brand management is needed. This means seeing both as examples of inference management. In this article the authors first interpret findings from their research on utility service guarantees in light of the model of service delivery proposed by Smith, Bolton, and Wagner. The authors then situate this model within their own model of utility brand management. The resulting approach can be used by a utility to evaluate whether and how to adopt service guarantees as part of its overall branding strategy.

  11. Understanding consumer response to service guarantees

    International Nuclear Information System (INIS)

    Whether the goal is customer loyalty to a competitive energy service provider or customer satisfaction with monopoly distribution services, service guarantees must be seen as one way a company can communicate with its customers about the relationship it has (or wants) with them. In short, service guarantees must be evaluated as part of a company's overall brand management strategy. To do this, a conceptual framework within which to develop and link models of service delivery and brand management is needed. This means seeing both as examples of inference management. In this article the authors first interpret findings from their research on utility service guarantees in light of the model of service delivery proposed by Smith, Bolton, and Wagner. The authors then situate this model within their own model of utility brand management. The resulting approach can be used by a utility to evaluate whether and how to adopt service guarantees as part of its overall branding strategy

  12. Security Guarantees and Nuclear Non-Proliferation

    International Nuclear Information System (INIS)

    The purpose of this paper is to discuss the value of 'security guarantees', that is, positive security assurances that include a formal or informal defense commitment, in preventing nuclear proliferation. It demonstrates that such guarantees have proven to be a very effective instrument in preventing States from going nuclear. It would thus seem logical to reinforce or extend them. However, this path is fraught with obstacles and dilemmas

  13. CREDIT SYSTEM AND CREDIT GUARANTEE PROGRAMS

    OpenAIRE

    Turgay GECER

    2012-01-01

    Credit system is an integrated architecture consisted of financial information, credit rating, credit risk management, receivables and credit insurance systems, credit derivative markets and credit guarantee programs. The main purpose of the credit system is to provide the functioning of all credit channels and to make it easy to access of credit sources demanded by all of real and legal persons in any economic system. Credit guarantee program, the one of prominent elements of the credit syst...

  14. MODELLING THE GUARANTEE LIABILITY UNDER UNIT-LINKED CONTRACTS

    OpenAIRE

    Cristina CIUMAȘ; Diana-Maria CHIȘ

    2015-01-01

    Unit-linked contracts are wrapped with some death, maturity and accumulation guarantees such as the guaranteed minimum maturity benefit, the guaranteed minimum death benefit, the guaranteed minimum accumulation benefit, the guaranteed minimum income benefit, and/or the guaranteed minimum surrender benefit. According to Romanian legislation which regulates the unit-linked life insurance market, unit-linked life insurance contracts pass most of the investment risk to the policyholder and involv...

  15. Probabilistic Recovery Guarantees for Sparsely Corrupted Signals

    CERN Document Server

    Pope, Graeme; Studer, Christoph

    2012-01-01

    We consider the recovery of sparse signals subject to sparse interference, as introduced in Studer et al., IEEE Trans. IT, 2012. We present novel probabilistic recovery guarantees for this framework, covering varying degrees of knowledge of the signal and interference support, which are relevant for a large number of practical applications. Our results assume that the sparsifying dictionaries are solely characterized by coherence parameters and we require randomness only in the signal and/or interference. The obtained recovery guarantees show that one can recover sparsely corrupted signals with overwhelming probability, even if the sparsity of both the signal and interference scale (near) linearly with the number of measurements.

  16. Large-Scale Similarity Joins With Guarantees

    DEFF Research Database (Denmark)

    Pagh, Rasmus

    2015-01-01

    The ability to handle noisy or imprecise data is becoming increasingly important in computing. In the database community the notion of similarity join has been studied extensively, yet existing solutions have offered weak performance guarantees. Either they are based on deterministic filtering te...

  17. 46 CFR 298.36 - Guarantee Fee.

    Science.gov (United States)

    2010-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VESSEL FINANCING ASSISTANCE OBLIGATION GUARANTEES Documentation... generally shall vary inversely with the ratio of Equity to Long-Term Debt (Variable Rate) of the Person who... computation of Equity and Long-Term Debt considered necessary to reflect more accurately the...

  18. Insights into tRNA-Dependent Amidotransferase Evolution and Catalysis from the Structure of the Aquifex aeolicus Enzyme

    OpenAIRE

    WU, Jing; Bu, Weishu; Sheppard, Kelly; Kitabatake, Makoto; Kwon, Suk-Tae; Söll, Dieter; Smith, Janet L.

    2009-01-01

    Many bacteria form Gln-tRNAGln and Asn-tRNAAsn by conversion of the misacylated Glu-tRNAGln and Asp-tRNAAsn species catalyzed by the GatCAB amidotransferase in the presence of ATP and an amide donor (glutamine or asparagine). Here we report the crystal structures of GatCAB from the hyperthermophilic bacterium Aquifex aeolicus complexed with glutamine, asparagine, aspartate, ADP, or ATP. In contrast to the Staphylococcus aureus GatCAB, the A. aeolicus enzyme formed acyl-enzyme intermediates wi...

  19. Linezolid resistant Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Pavani Gandham

    2014-08-01

    Full Text Available Linezolid is the only antibiotic available as an oral formulation for resistant staphylococcal infections. It is effective in skin and soft tissue infections, nosocomial pneumonias including VAP, infective endocarditis and MRSA meningitis. It is also effective in the eradication of both nasal and throat colonization of MRSA. Its high bioavailability and post antibiotic effect, ease of switching to oral therapy during its use and the fact that it can be used in patients of all ages, also in patients with liver disease and poor kidney function and its increased effectiveness over glycopeptides makes this drug a precious drug in the treatment of resistant staphylococcal infections. Linezolid resistance in staphylococcus is defined as a linezolid MIC of and #8805;8 mg/L. Reported Linezolid resistance in India and elsewhere is 2-20%. There is clonal dissemination of Linezolid Resistant Staphylococcus aureus (LRSA within or across health care settings which demands continuous surveillance to determine the emergent risk of resistance strains and to establish guidelines for appropriate use. Clinical laboratories should confirm any LRSA preferably by a second method, prior to using linezolid for serious infections. Effective surveillance, more judicious use of this antibiotic, avoiding linezolid usage for empiric therapy in hospital acquired staphylococcus infections, optimization of the pharmacological parameters of the antibiotics in specific clinical situation, decreasing bacterial load by timely surgical debridement or drainage of collections, use of combination therapies would prevent the emergence of resistance to linezolid in staphylococcus aureus. [Int J Res Med Sci 2014; 2(4.000: 1253-1256

  20. 78 FR 30854 - Notice of Funding Availability (NOFA) for Loan Guarantees Under Section 538 Guaranteed Rural...

    Science.gov (United States)

    2013-05-23

    ... in conjunction with the loan guarantee, including Home Investment Partnerships Program (HOME) grant... Protection Act (NEPA) and completed Form RD 3565-1 for the selected project. The Agency will prioritize...

  1. 76 FR 30641 - Notice of Funding Availability (NOFA) for Loan Guarantees Under Section 538 Guaranteed Rural...

    Science.gov (United States)

    2011-05-26

    ... the loan guarantee, including Home Investment Partnership Program (HOME) grant funds, tax exempt bonds... (NEPA) and completed Form RD 3565-1 for the selected project. The Agency will prioritize the...

  2. GAMMA Radiation Effect On Staphylococcus aureus (ATCC 19095) in Cheese MINAS FRESCALIRRADIATED

    International Nuclear Information System (INIS)

    Milk is an excellent medium of culture for development of Staphylococcus aureus. Gamma radiation can be an alternative method to guarantee the safety of the contaminated cheeses. The objective of this research was determine the effects of the gamma radiation on the resistance of S.aureus (ATCC 19095) in cheese Minas Frescalirradiated. The cheeses elaborated in the Laboratory of Food Irradiation of CENA/USP, were contaminated during their production with 10 6 CFU/mL of culture of S.aureus (ATCC 19095). The cheeses were irradiated with 0; 1; 2; 3 and 4 kGy, maintained under refrigeration condition (50C) and analyzed at 1, 7 and 14 days of storage. The evaluation microbiology was made through the S.aureus survival analysis using Baird Parker selective medium and confirmative test of coagulase, catalase and fermentation aerobics of the manitol. The capacity of enterotoxins production by irradiated S.aureus was detected by the method of Passive Reverse Agglutination Latex. Results showed that 3 kGy is enough to destroy the S.aureus and 2 kGy to inhibited its toxins production

  3. Gamma radiation effect on staphylococcus aureus (atcc 19095) in cheese minas frescal irradiated

    International Nuclear Information System (INIS)

    Milk is an excellent medium of culture for development of staphylococcus aureus. Gamma Radiation can be an alternative method to guarantee the safety of the comtamined cheeses. The objective of this research was determine the effects of the gamma radiation on the resistance of S.aureus (atcc 19095) in cheese minas frescal irradiated. The cheeses elaborated in the Laboratory of food irradiation of cena/usp, were contaminated during their production with 10 6 cfu/ml of culture of s.aureus (atcc 19095). The cheeses were irradiated with 0; 1; 2; 3 and 4 kgy, maintained under refrigeration condition (± 50c) and analyzed at 1, 7 and 14 days of storage. The evaluation microbiology was made through the s.aureus survival analysis using baird parker selective medium and confirmative test of coagulase, catalase and fermentation aerobics of the manitol. The capacity of enterotoxins production by irradiated s.aureus was detected by the method of passive reverse agglutination latex. results showed that 3 kgy is enough to destroy the s.aureus and 2 kgy to inhibited its toxins production

  4. Enigma: Decentralized Computation Platform with Guaranteed Privacy

    OpenAIRE

    Zyskind, Guy; Nathan, Oz; Pentland, Alex

    2015-01-01

    A peer-to-peer network, enabling different parties to jointly store and run computations on data while keeping the data completely private. Enigma's computational model is based on a highly optimized version of secure multi-party computation, guaranteed by a verifiable secret-sharing scheme. For storage, we use a modified distributed hashtable for holding secret-shared data. An external blockchain is utilized as the controller of the network, manages access control, identities and serves as a...

  5. Learning Good Edit Similarities with Generalization Guarantees

    OpenAIRE

    Bellet, Aurélien; Habrard, Amaury; Sebban, Marc

    2011-01-01

    Similarity and distance functions are essential to many learning algorithms, thus training them has attracted a lot of interest. When it comes to dealing with structured data (e.g., strings or trees), edit similarities are widely used, and there exists a few methods for learning them. However, these methods offer no theoretical guarantee as to the generalization performance and discriminative power of the resulting similarities. Recently, a theory of learning with good similarity functions wa...

  6. Improved Practical Matrix Sketching with Guarantees

    OpenAIRE

    Desai, Amey; Ghashami, Mina; Phillips, Jeff M.

    2015-01-01

    Matrices have become essential data representations for many large-scale problems in data analytics, and hence matrix sketching is a critical task. Although much research has focused on improving the error/size tradeoff under various sketching paradigms, the many forms of error bounds make these approaches hard to compare in theory and in practice. This paper attempts to categorize and compare most known methods under row-wise streaming updates with provable guarantees, and then to tweak some...

  7. China and the Multilateral Investment Guarantee Agency

    OpenAIRE

    Yushu Feng

    1997-01-01

    The Multilateral Investment Guarantee Agency (MIGA), established in 1988, is part of the World Bank Group. Its mandate is to promote foreign investment in its member countries by providing political risk insurance and technical assistance to promote investment. In developing this case study of China's evolving relationship with MIGA, the author asked: Why did China decide to seek membership in MIGA? What was the outcome of the relationship between China and MIGA? How has that relationship aff...

  8. Staphylococcus aureus triggered reactive arthritis.

    OpenAIRE

    Siam, A R; M. Hammoudeh

    1995-01-01

    OBJECTIVES--To report two patients who developed reactive arthritis in association with Staphylococcus aureus infection. METHODS--A review of the case notes of two patients. RESULTS--Two adult female patients have developed sterile arthritis in association with Staph aureus infection. The first patient has had two episodes of arthritis; the first followed olecranon bursitis, the second followed infection of a central venous catheter used for dialysis. The second patient developed sterile arth...

  9. Staphylococcus aureus and sore nipples.

    OpenAIRE

    Livingstone, V. H.; Willis, C. E.; Berkowitz, J

    1996-01-01

    OBJECTIVE: To correlate clinical symptoms and signs of sore nipples with the presence of Staphylococcus aureus and to determine the probability of mothers having S aureus-infected nipples when these local symptoms and signs are found. DESIGN: Two cohorts of consecutive patients were enrolled regardless of presenting complaint. A questionnaire was administered to determine the presence and severity of sore nipples. Objective findings on breast examination were documented. A nipple swab was tak...

  10. 75 FR 63419 - Surety Bond Guarantee Program; Timber Sales

    Science.gov (United States)

    2010-10-15

    ...The Small Business Administration (SBA) proposes to amend its Surety Bond Guarantee Program rules to guarantee performance bonds for timber sale contracts awarded by the Federal Government or other public or private...

  11. 76 FR 9962 - Surety Bond Guarantee Program; Timber Sales

    Science.gov (United States)

    2011-02-23

    ...The Small Business Administration (SBA) is issuing this final rule to amend its Surety Bond Guarantee Program rules to guarantee bid and performance bonds for timber sale contracts awarded by the Federal Government or other public and private...

  12. 29 CFR 541.604 - Minimum guarantee plus extras.

    Science.gov (United States)

    2010-07-01

    ... SALES EMPLOYEES Salary Requirements § 541.604 Minimum guarantee plus extras. (a) An employer may provide... includes a guarantee of at least the minimum weekly required amount paid on a salary basis regardless of... 29 Labor 3 2010-07-01 2010-07-01 false Minimum guarantee plus extras. 541.604 Section...

  13. 7 CFR 4279.149 - Personal and corporate guarantee.

    Science.gov (United States)

    2010-01-01

    ...-COOPERATIVE SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE GUARANTEED LOANMAKING Business and Industry Loans § 4279.149 Personal and corporate guarantee. (a) Unconditional personal and corporate... guarantees may also be required of parent, subsidiaries, or affiliated companies (owning less than a...

  14. 10 CFR 609.10 - Loan Guarantee Agreement.

    Science.gov (United States)

    2010-01-01

    ... accepted accounting principles and practices. The non-guaranteed portion (if any) of any Guaranteed...; (7) The loan guarantee does not finance, either directly or indirectly, tax-exempt debt obligations... made during the regular business hours of the Borrower, Eligible Lender or other Holder, or other...

  15. 77 FR 58991 - State-Level Guarantee Fee Pricing

    Science.gov (United States)

    2012-09-25

    ... AGENCY State-Level Guarantee Fee Pricing AGENCY: Federal Housing Finance Agency. ACTION: Notice; input... Mac of developing appropriate risk-based guarantee fee pricing by state. FHFA's proposal described... subjects. After reviewing the public input and determining a final state-level guarantee fee pricing...

  16. 7 CFR 762.145 - Restructuring guaranteed loans.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 7 2010-01-01 2010-01-01 false Restructuring guaranteed loans. 762.145 Section 762..., DEPARTMENT OF AGRICULTURE SPECIAL PROGRAMS GUARANTEED FARM LOANS § 762.145 Restructuring guaranteed loans. (a... approval of the Agency for all restructuring actions; and, (ii) Provide the items in paragraph (b) and...

  17. 29 CFR 4022.62 - Estimated guaranteed benefit.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Estimated guaranteed benefit. 4022.62 Section 4022.62 Labor... PAYABLE IN TERMINATED SINGLE-EMPLOYER PLANS Benefit Reductions in Terminating Plans § 4022.62 Estimated guaranteed benefit. (a) General. The estimated guaranteed benefit payable with respect to each...

  18. 7 CFR 1717.1207 - RUS obligations under loan guarantees.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 11 2010-01-01 2010-01-01 false RUS obligations under loan guarantees. 1717.1207... ELECTRIC LOANS Settlement of Debt § 1717.1207 RUS obligations under loan guarantees. Nothing in this subpart affects the obligations of RUS under loan guarantee commitments it has made to the...

  19. Collagen binding to Staphylococcus aureus

    International Nuclear Information System (INIS)

    Staphylococcus aureus can bind soluble collagen in a specific, saturable manner. We have previously shown that some variability exists in the degree of collagen binding between different strains of heat-killed, formaldehyde-fixed S. aureus which are commercially available as immunologic reagents. The present study demonstrates that live S. aureus of the Cowan 1 strain binds amounts of collagen per organism equivalent to those demonstrated previously in heat-killed, formaldehyde-fixed bacteria but has an affinity over 100 times greater, with Kd values of 9.7 X 10(-11) M and 4.3 X 10(-8) M for live and heat-killed organisms, respectively. Studies were also carried out with S. aureus killed by ionizing radiation, since this method of killing the organism seemed less likely to alter the binding moieties on the surface than did heat killing. Bacteria killed by exposure to gamma radiation bound collagen in a manner essentially indistinguishable from that of live organisms. Binding of collagen to irradiated cells of the Cowan 1 strain was rapid, with equilibrium reached by 30 min at 22 degrees C, and was fully reversible. The binding was not inhibited by fibronectin, fibrinogen, C1q, or immunoglobulin G, suggesting a binding site for collagen distinct from those for these proteins. Collagen binding was virtually eliminated in trypsin-treated organisms, indicating that the binding site has a protein component. Of four strains examined, Cowan 1 and S. aureus ATCC 25923 showed saturable, specific binding, while strains Woods and S4 showed a complete lack of binding. These results suggest that some strains of S. aureus contain high-affinity binding sites for collagen. While the number of binding sites per bacterium varied sixfold in the two collagen-binding strains, the apparent affinity was similar

  20. 75 FR 4707 - Continuous Construction-Permanent Loan Guarantees Under the Section 538 Guaranteed Rural Rental...

    Science.gov (United States)

    2010-01-29

    ...; ] DEPARTMENT OF AGRICULTURE Rural Housing Service 7 CFR Part 3565 RIN-0575-AC80 Continuous Construction... guarantee on construction advances and the permanent financing phase of a project. In addition to the... Executive Order 12988, Civil Justice Reform. If this rule is adopted: (1) Unless otherwise...

  1. Host-adaptive evolution of Staphylococcus aureus

    OpenAIRE

    Lowder, Bethan Victoria

    2011-01-01

    Staphylococcus aureus is a notorious human pathogen associated with severe nosocomial and community-acquired infections. In addition, S. aureus is a major cause of animal diseases including skeletal infections of poultry and bovine and ovine mastitis, which are a large economic burden on the broiler chicken and dairy farming industries. The population structure of S. aureus associated with humans has been well studied. However, despite the prevalence of S. aureus infections in ...

  2. Genome Sequence of the Clinical Isolate Staphylococcus aureus subsp. aureus Strain UAMS-1

    OpenAIRE

    Sassi, Mohamed; Sharma, Deepak; Brinsmade, Shaun ,; Felden, Brice; Augagneur, Yoann

    2015-01-01

    We report here the draft genome sequence of Staphylococcus aureus subsp. aureus strain UAMS-1. UAMS-1 is a virulent oxacillin-susceptible clinical isolate. Its genome is composed of 2,763,963 bp and will be useful for further gene expression analysis using RNA sequencing (RNA-seq) technology. S taphylococcus aureus is an opportunistic human bacterial pathogen responsible for nosocomial and community-associated infections. S. aureus subsp. aureus strain UAMS-1 was originally isolated from the ...

  3. Guaranteed clustering and biclustering via semidefinite programming

    CERN Document Server

    Ames, Brendan P W

    2012-01-01

    Identifying clusters of similar objects in data plays a significant role in a wide range of applications. As a model problem for clustering, we consider the densest k-disjoint-clique problem, whose goal is to identify the collection of k disjoint cliques of a given weighted complete graph maximizing the sum of the densities of the complete subgraphs induced by these cliques. In this paper, we establish conditions ensuring exact recovery of the densest k cliques of a given graph from the optimal solution of a particular semidefinite program. In particular, the semidefinite relaxation is exact for input graphs corresponding to data consisting of k large, distinct clusters and a smaller number of outliers. This approach also yields a semidefinite relaxation for the biclustering problem with similar recovery guarantees. Given a set of objects and a set of features exhibited by these objects, biclustering seeks to simultaneously group the objects and features according to their expression levels. This problem may ...

  4. Guaranteeing CERN’s excellence: consolidate experience

    CERN Multimedia

    Staff Association

    2014-01-01

    For its missions CERN requires staff with solid experience in all its domains of activity The Organization has several missions: fundamental research, technical developments and innovation, training the several hundreds of associates, fellows and students, while at the same time taking care of more than 10000 users. In order to guarantee excellence in all of these areas, CERN has to put in place an efficient personnel policy. Such a policy must allow the Organization to recruit collaborators with the highest competence from all Member States, and to keep and motivate them during their entire professional career. But, more importantly, the Organization has to be able to count on a stable workforce. It needs staff with experience gained over a long period in the fields of accelerators, detectors, and operating procedures, if it is to fulfil successfully its important mission of training and knowledge transfer, which is very much appreciated by our Member states, since it highlights a visible return on invest...

  5. Essence and classification of legal guarantees of creditors’ rights

    Directory of Open Access Journals (Sweden)

    Aleksandra M. Sil’chenko

    2014-01-01

    Full Text Available Objective to conduct a comprehensive study of the concept of legal guarantees of creditorsrsquo rights and to identify some features of guarantees on the basis of the presented classification. Methods general scientific deduction comparative and system analysis formal logical method and special comparative legal and structuralfunctional methods. Results basing on the evaluation of different scientistsrsquo opinions the definition of the notion laquolegal guarantees of creditorsrsquo rightsraquo is given. Four classifications of legal guarantees of creditorsrsquo rights are proposed. Scientific novelty author39s definition of the notion laquolegal guarantees of creditorsrsquo rightsraquo is given. The essence of legal guarantees in general and legal rights of creditors in particular is defined. Classifications of legal guarantees of creditorsrsquo rights are given by the content and methods of implementation of creditorsrsquo rights guarantees by forms of providing guarantees to creditors general and special guarantees by the form of termination of legal personrsquos activity. The classifications are described in detail through examples. Practical value the results of this research can be used in scientificresearch activity. Theoretical conclusions formulated in the work can be used in the process of improving of the existing legislation and practice of its application.

  6. Staphylococcus aureus and hand eczema severity

    DEFF Research Database (Denmark)

    Haslund, P; Bangsgaard, N; Jarløv, J O;

    2009-01-01

    BACKGROUND: The role of bacterial infections in hand eczema (HE) remains to be assessed. OBJECTIVES: To determine the prevalence of Staphylococcus aureus in patients with HE compared with controls, and to relate presence of S. aureus, subtypes and toxin production to severity of HE. METHODS......: Bacterial swabs were taken at three different visits from the hand and nose in 50 patients with HE and 50 controls. Staphylococcus aureus was subtyped by spa typing and assigned to clonal complexes (CCs), and isolates were tested for exotoxin-producing S. aureus strains. The Hand Eczema Severity Index...... was used for severity assessment. RESULTS: Staphylococcus aureus was found on the hands in 24 patients with HE and four controls (P aureus was found to be related to increased severity of the eczema (P aureus types on the hands...

  7. Guaranteed investment contracts: distributed and undistributed excess return

    OpenAIRE

    Kristian R. Miltersen; Persson, Svein-Arne

    2000-01-01

    Annual minimum rate of return guarantees are analyzed together with rules for distribution of positive excess return, i.e. investment returns in excess of the guaranteed minimum return. Together with the level of the annual minimum rate of return guarantee both the customer's and the insurer's fractions of the positive excess return are determined so that the market value of the insurer's capital inflow (determined by the fraction of the positive excess return) equals the market value of the ...

  8. Guaranteed capacity of hydro power plants in Germany and Austria

    International Nuclear Information System (INIS)

    Balancing fluctuations caused by renewable energy play an important role in the current transition of energy supply systems. Hydro power plants provide both: guaranteed capacity as well as renewability. In Germany and Austria definitions of how to quantify the guaranteed capacity of hydro power plants are well established to calculate the guaranteed capacity for an individual power plant. The definitions are challenged against real examples.

  9. Switching Fuzzy Guaranteed Cost Control for Nonlinear Networked Control Systems

    OpenAIRE

    Linqin Cai; Zhuo Yang; Jimin Yu; Zhenhua Zhang

    2014-01-01

    This paper deals with the problem of guaranteed cost control for a class of nonlinear networked control systems (NCSs) with time-varying delay. A guaranteed cost controller design method is proposed to achieve the desired control performance based on the switched T-S fuzzy model. The switching mechanism is introduced to handle the uncertainties of NCSs. Based on Lyapunov functional approach, some sufficient conditions for the existence of state feedback robust guaranteed cost controller are p...

  10. Valuation and Efficient Allocation of GSM Export Credit Guarantees

    OpenAIRE

    Diersen, Matthew A.; Sherrick, Bruce J.

    2005-01-01

    Estimates of country-level loan default distributions are developed and used in a loan guarantee model to value the contingent liability of USDA's General Sales Manager (GSM) export credit guarantee portfolio. The results quantify the relationship between increasing guarantee coverage and the resulting actuarial liability to the government. Optimal coverage levels and optimal country-level allocations are determined for given policy objectives and coverage totals. Findings reveal that the gov...

  11. Genome sequence of type strain of Staphylococcus aureus subsp. aureus

    OpenAIRE

    Kim, Bong-Soo; Yi, Hana; Chun, Jongsik; Cha, Chang-Jun

    2014-01-01

    Background Staphylococcus aureus is a pathogen that causes food poisoning and community-associated infection with antibiotic resistance. This species is an indigenous intestinal microbe found in infants and not found in adult intestine. The relatively small genome size and rapid evolution of antibiotic resistance genes in the species have been drawing an increasing attention in public health. To extend our understanding of the species and use the genome data for comparative genomic studies, w...

  12. 7 CFR 1493.430 - Application for a payment guarantee.

    Science.gov (United States)

    2010-01-01

    ... exporter and the importer. If the commodity was sold on the basis of CFR or CIF, the actual (if known at... PROGRAMS CCC Supplier Credit Guarantee Program Operations § 1493.430 Application for a payment guarantee..., the actual (if known at the time of application) or estimated value of marine and war risk...

  13. 75 FR 25833 - Information Collection; Guaranteed Loan Making and Servicing

    Science.gov (United States)

    2010-05-10

    ... contacting Trent Rogers at the above address. FOR FURTHER INFORMATION CONTACT: Trent Rogers, Senior Loan... Farm Service Agency Information Collection; Guaranteed Loan Making and Servicing AGENCY: Farm Service... the Guaranteed Farm Loan Program. DATES: We will consider comments that we receive by July 9,...

  14. Increasing Social Inclusion through Social Guarantees : A Policy Note

    OpenAIRE

    World Bank

    2007-01-01

    This policy note seeks to contribute to the consideration of social guarantees as tools for the design and/or monitoring of social policy and service delivery. It proposes a range of options to improve the delivery of and access to social services that can further social inclusion and democratic governance. The application of a social guarantee framework to social policy could significantl...

  15. Guaranteed Cost LQG Control of Uncertain Linear Quantum Stochastic Systems

    OpenAIRE

    A. J. SHAIJU; Petersen, I. R.; James, M R

    2008-01-01

    In this paper, we formulate and solve a guaranteed cost control problem for a class of uncertain linear stochastic quantum systems. For these quantum systems, a connection with an associated classical (non-quantum) system is first established. Using this connection, the desired guaranteed cost results are established. The theory presented is illustrated using an example from quantum optics.

  16. System Guarantee for Social Equity in Today’s China

    Directory of Open Access Journals (Sweden)

    Luyan MA

    2012-11-01

    Full Text Available

    Social equity is balanced and equal distribution of rights and interests. A sound system is the fundamental guarantee for social equity. Unfortunately, imperfection of the current system in China obstructed social equity in today’s China such as income distribution system, dual urbanrural system, market economic system, citizen rights and interests, protection system and social security system. To eliminate these barriers, strengthen system building and guarantee social equity, equity and justice should be taken as the primary goal of value for system building; a guarantee mechanism for social equity system should be built focusing on fairness in rights, opportunities, rules and distribution; and a system to guarantee social equity should be built and perfected with multi-tiered systems including macro basic system, medium operation system and microcosmic concrete system.

    Key words: Social equity; System; Guarantee

  17. Evasion of Neutrophil Killing by Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Will A. McGuinness

    2016-03-01

    Full Text Available Staphylococcus aureus causes many types of infections, ranging from self-resolving skin infections to severe or fatal pneumonia. Human innate immune cells, called polymorphonuclear leukocytes (PMNs or neutrophils, are essential for defense against S. aureus infections. Neutrophils are the most prominent cell type of the innate immune system and are capable of producing non-specific antimicrobial molecules that are effective at eliminating bacteria. Although significant progress has been made over the past few decades, our knowledge of S. aureus-host innate immune system interactions is incomplete. Most notably, S. aureus has the capacity to produce numerous molecules that are directed to protect the bacterium from neutrophils. Here we review in brief the role played by neutrophils in defense against S. aureus infection, and correspondingly, highlight selected S. aureus molecules that target key neutrophil functions.

  18. Population structure of Staphylococcus aureus in China

    OpenAIRE

    Yan, Xiaomei

    2015-01-01

    The present PhD research was aimed at analysing the population structure of Staphylococcus aureus in China. Between 2000 and 2005 we found that patients from a single Chinese hospital showed increasing trends in antimicrobial resistance. Among methicillin-resistant S. aureus (MRSA), resistance against rifampicin doubled to 68%. Staphylococcal food poisoning (SFP) is frequent in China. Two predominant S. aureus lineages, ST6 and ST943, were identified causing outbreaks of SFP in Southern China...

  19. Immunomodulation and Disease Tolerance to Staphylococcus aureus

    OpenAIRE

    Zhigang Li; Peres, Adam G.; Andreea C. Damian; Joaquín Madrenas

    2015-01-01

    The Gram-positive bacterium Staphylococcus aureus is one of the most frequent pathogens that causes severe morbidity and mortality throughout the world. S. aureus can infect skin and soft tissues or become invasive leading to diseases such as pneumonia, endocarditis, sepsis or toxic shock syndrome. In contrast, S. aureus is also a common commensal microbe and is often part of the human nasal microbiome without causing any apparent disease. In this review, we explore the immunomodulation and d...

  20. Mastite com lesões sistêmicas por Staphylococus aureus subesp. aureus em coelhos Mastitis with systemic lesions due to Staphylococus aureus subesp. aureus in rabbits

    OpenAIRE

    Sandra Davi Traverso; Leonardo da Cunha; Joaquim César Teixeira Fernandes; Alexandre Paulino Loretti; Adriana Rhoden; Elsio Wunder Jr.; David Driemeier

    2003-01-01

    Em uma criação composta por 1800 coelhos, 33% das matrizes apresentaram mastite e lesões cutâneas crostosas e purulentas. Estes animais apresentavam-se entre 10 a- 12 meses de idade e em segunda parição. Quinze coelhos afetados foram sacrificados e necropsiados. Na necropsia, além das lesões cutâneas haviam microabscessos em diversos órgãos. Das amostras coletadas isolou-se Staphylococcus aureus subesp. aureus. S. aureus subesp. aureus também foi isolado de "swab" nasal coletado do tratador e...

  1. Immunomodulation and Disease Tolerance to Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Zhigang Li

    2015-11-01

    Full Text Available The Gram-positive bacterium Staphylococcus aureus is one of the most frequent pathogens that causes severe morbidity and mortality throughout the world. S. aureus can infect skin and soft tissues or become invasive leading to diseases such as pneumonia, endocarditis, sepsis or toxic shock syndrome. In contrast, S. aureus is also a common commensal microbe and is often part of the human nasal microbiome without causing any apparent disease. In this review, we explore the immunomodulation and disease tolerance mechanisms that promote commensalism to S. aureus.

  2. Methicillin-Resistant Staphylococcus aureus (MRSA) Treatment

    Science.gov (United States)

    ... Laboratory of Bacteriology Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) Antibacterial Resistance Leadership Group (ARLG) NIAID Antimicrobial Resistance Funding Information ...

  3. Methicillin-Resistant Staphylococcus aureus (MRSA) Diagnosis

    Science.gov (United States)

    ... Laboratory of Bacteriology Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) Antibacterial Resistance Leadership Group (ARLG) NIAID Antimicrobial Resistance Funding Information ...

  4. Staphylococcus aureus small colony variants in diabetic foot infections

    OpenAIRE

    Cervante-García, Estrella; García-Gonzalez, Rafael; Reyes-Torres, Angélica; Resendiz-Albor, Aldo Arturo; Salazar-Schettino, Paz María

    2015-01-01

    Background: Staphylococcus aureus (S. aureus) is one of the major pathogens causing chronic infections. The ability of S. aureus to acquire resistance to a diverse range of antimicrobial compounds results in limited treatment options, particularly in methicillin-resistant S. aureus (MRSA). A mechanism by which S. aureus develops reduced susceptibility to antimicrobials is through the formation of small colony variants (SCVs). Infections by SCVs of S. aureus are an upcoming problem due to diff...

  5. Evaluation of Two New Chromogenic Media, CHROMagar MRSA and S. aureus ID, for Identifying Staphylococcus aureus and Screening Methicillin-Resistant S. aureus

    OpenAIRE

    Hedin, Göran; Fang, Hong

    2005-01-01

    Thirty-nine methicillin-resistant Staphylococcus aureus (MRSA) isolates with diverse genetic backgrounds and two reference strains were correctly identified as S. aureus on CHROMagar MRSA and S. aureus ID media. Growth inhibition on CHROMagar MRSA was noted. A combination of cefoxitin disk and S. aureus ID was found suitable for rapid MRSA screening.

  6. Price-Matching Guarantees and Entry Deterrence under Incomplete Information

    OpenAIRE

    Liping Zhang

    2012-01-01

    This paper studies price-matching guarantees in a market where entrant does not have perfect information about incumbent¡¯s cost. The low-cost incumbent can adopt price-matching guarantees as a signal to distinguish itself from the high-cost type and thus effectively deter entry. On the other hand, the high-cost incumbent can successfully fool the potential entrant under certain conditions. Compared with the equilibriums in situations where the option of offering a price guarantee is not avai...

  7. Empirical research on the SMEs' credit-guarantee in China

    Institute of Scientific and Technical Information of China (English)

    贾生华; 陈宏辉

    2002-01-01

    After the authors' thorough study of the experiments of other countries and regions, it is posed that credit-guarantee is the key for solving the problem of the difficulties of SMEs' financing. Based on two surveys and interviews with 57 SMEs and some commercial banks' staff, this study on the practice and implement action of SMEs' credit-guarantee revealed some problems existing in the practical process. A series of policy suggestions are given for improving the SMEs' credit-guarantee in China.

  8. Empirical research on the SMEs' credit-guarantee in China

    Institute of Scientific and Technical Information of China (English)

    贾生华; 陈宏辉

    2002-01-01

    After the authors' thorough study of the experiments of other countries and regions, it is posed that credit-guarantee is the key for solving the problem of the difficulties of SMEs' financing.Based on two surveys and interviews with 57 SMEs and some commercial banks' staff, this study on the practice and implement action of SMEs' credit-guarantee revealed some problems existing in the practical process. A series of policy suggestions are given for improving the SMEs' credit-guarantee in China.

  9. The cell surface proteome of Staphylococcus aureus

    NARCIS (Netherlands)

    Dreisbach, Annette; van Dijl, Jan Maarten; Buist, Girbe

    2011-01-01

    The Gram-positive bacterium Staphylococcus aureus is a wide spread opportunistic pathogen that can cause a range of life-threatening diseases. To obtain a better understanding of the global mechanisms for pathogenesis and to identify novel targets for therapeutic interventions, the S. aureus proteom

  10. Population structure of Staphylococcus aureus in China

    NARCIS (Netherlands)

    Yan, Xiaomei

    2015-01-01

    The present PhD research was aimed at analysing the population structure of Staphylococcus aureus in China. Between 2000 and 2005 we found that patients from a single Chinese hospital showed increasing trends in antimicrobial resistance. Among methicillin-resistant S. aureus (MRSA), resistance again

  11. [Waiting lists guarantee in health care. Some theoretical reflections].

    Science.gov (United States)

    Piene, H; Hauge, H K; Nyen, P A

    1997-01-30

    In 1990 a "waiting list guarantee" was introduced in the Norwegian health care system to secure treatment within six months for patients belonging to priority group II. (Priority group II are patients in need of treatment to avoid health hazards or serious long-term effects.) This guarantee has been difficult to honour and has caused considerable political unrest in the recent years. In an attempt to reform the guarantee, an analysis of our hospitals' capacity problems has been carried out, based on the general theory of queues. One result was that in order to fulfill the guarantee it is necessary to drastically reduce the queues and increase the capacity to deliver health services. This article presents the reasoning behind the analysis, in order to demonstrate the necessary foundation for a health policy that aims to reduce the time Norwegian patients have to wait for treatment in hospital. PMID:9064860

  12. Optimal consumption, investment and life insurance with surrender option guarantee

    DEFF Research Database (Denmark)

    Kronborg, Morten Tolver; Steffensen, Mogens

    2015-01-01

    We consider an investor, with an uncertain lifetime, endowed with deterministic laboor income, who has the possibility to continuously invest in a Black–Scholes market and to buy life insurance or annuities. We solve the optimal consumption, investment and life insurance problem when the investor...... is restricted to fulfil an American capital guarantee. By allowing the guarantee to depend, in a very general way, on the past we include, among other possibilities, the interesting case of a minimum rate of return guarantee, commonly offered by pension companies. The optimal strategies turn out to...... be on option-based portfolio insurance form, but since the capital guarantee is valid at every intermediate point in time, re-calibration is needed whenever the constraint is active....

  13. 75 FR 52429 - Guaranteed Single Family Housing Loans

    Science.gov (United States)

    2010-08-26

    ...The Rural Housing Service (RHS) is amending its regulations to add new servicing options to the Single Family Housing Guaranteed Loan Program (SFHGLP) that lenders may utilize while still maintaining the SFHGLP loan guarantee. The Agency will allow lenders to extend loans for a term of up to 40 years from the date of modification. The Agency also will allow lenders to advance funds on behalf......

  14. Credit Guarantees and Subsidies when Lender has a Market Power

    OpenAIRE

    Janda, Karel

    2011-01-01

    Provision of credit guarantees or subsidies may remove an adverse selection leading to credit rationing. This paper concentrates on comparison of government budget costs of credit guarantees and subsidies in a monopolistic credit market. Different opportunity costs among entrepreneurs, which reflect different mixes of general and human specific capital, generate different outcomes in the model. As long as the participation costs of low-risk entrepreneurs are sufficiently close to the particip...

  15. DEPOSIT GUARANTEE SCHEMES JOIN FINANCIAL SAFETY-NET

    OpenAIRE

    Eugen Dijmarescu

    2011-01-01

    Deposit Guarantee Schemes (DGS) become more visible under the current conditions of the world financial markets. If those have played in the past a rather discreet role for social stability through the function of pay box following a bank failure, the recent crisis put them in a new light, once the guarantee ceiling for eligible deposits grew in order to make for the accumulation of wealth worldwide and the increased risk incurred by the leverage of banking operations. Consequently, the resou...

  16. LEGAL GUARANTEES OF FARMERS’ TRADE UNIONS’ FUNCTIONING IN POLAND

    OpenAIRE

    Jakub Gołaś

    2016-01-01

    The main purpose of the article is to present legal basis and guarantees of the farmers’ trade unions’ functioning in Poland. The presented research was based on a thorough examination of national legal acts, which constitute both legal background and foundation of farmer’s unions’ activities and internal structure. The author tries to introduce both the individual legal and system guarantees which are dedicated to farmers’ organization and which exact acts shall...

  17. Customer intentions to invoke service guarantees: Do excellence in service recovery, type of guarantee, and cultural orientation matter?.

    OpenAIRE

    Van Vaerenbergh, Yves; De Keyser, Arne; Larivière, Bart

    2014-01-01

    Purpose – Many service providers feel confident about their service quality and thus offer service guarantees to their customers. Yet service failures are inevitable. As guarantees can only be invoked when customers report service failures, firms are given the opportunity to redress the original failure potentially influencing customer outcomes. The purpose of this paper is to provide the first empirical investigation of whether excellence in service recovery affects customers’ intentions to ...

  18. Genomic Analysis of Companion Rabbit Staphylococcus aureus

    Science.gov (United States)

    Holmes, Mark A.; Harrison, Ewan M.; Fisher, Elizabeth A.; Graham, Elizabeth M.; Parkhill, Julian; Foster, Geoffrey; Paterson, Gavin K.

    2016-01-01

    In addition to being an important human pathogen, Staphylococcus aureus is able to cause a variety of infections in numerous other host species. While the S. aureus strains causing infection in several of these hosts have been well characterised, this is not the case for companion rabbits (Oryctolagus cuniculus), where little data are available on S. aureus strains from this host. To address this deficiency we have performed antimicrobial susceptibility testing and genome sequencing on a collection of S. aureus isolates from companion rabbits. The findings show a diverse S. aureus population is able to cause infection in this host, and while antimicrobial resistance was uncommon, the isolates possess a range of known and putative virulence factors consistent with a diverse clinical presentation in companion rabbits including severe abscesses. We additionally show that companion rabbit isolates carry polymorphisms within dltB as described as underlying host-adaption of S. aureus to farmed rabbits. The availability of S. aureus genome sequences from companion rabbits provides an important aid to understanding the pathogenesis of disease in this host and in the clinical management and surveillance of these infections. PMID:26963381

  19. [Staphylococcus aureus and antibiotic resistance].

    Science.gov (United States)

    Sancak, Banu

    2011-07-01

    After the report of first case of methicillin-resistant Staphylococcus aureus (MRSA) in 1961, MRSA become a major problem worldwide. Over the last decade MRSA strains have emerged as serious pathogens in nosocomial and community settings. Glycopeptides (vancomycin and teicoplanin) are still the current mainstay of therapy for infections caused by MRSA. In the last decade dramatic changes have occurred in the epidemiology of MRSA infections. The isolates with reduced susceptibility and in vitro resistance to vancomycin have emerged. Recently, therapeutic alternatives such as quinupristin/dalfopristin, linezolid, tigecycline and daptomycin have been introduced into clinical practice for treating MRSA infections. Nevertheless, these drugs are only approved for certain indication and resistance has already been reported. In this review, the new information on novel drugs for treating MRSA infections and the resistance mechanisms of these drugs were discussed. PMID:21935792

  20. Exfoliative Toxins of Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Michal Bukowski

    2010-05-01

    Full Text Available Staphylococcus aureus is an important pathogen of humans and livestock. It causes a diverse array of diseases, ranging from relatively harmless localized skin infections to life-threatening systemic conditions. Among multiple virulence factors, staphylococci secrete several exotoxins directly associated with particular disease symptoms. These include toxic shock syndrome toxin 1 (TSST-1, enterotoxins, and exfoliative toxins (ETs. The latter are particularly interesting as the sole agents responsible for staphylococcal scalded skin syndrome (SSSS, a disease predominantly affecting infants and characterized by the loss of superficial skin layers, dehydration, and secondary infections. The molecular basis of the clinical symptoms of SSSS is well understood. ETs are serine proteases with high substrate specificity, which selectively recognize and hydrolyze desmosomal proteins in the skin. The fascinating road leading to the discovery of ETs as the agents responsible for SSSS and the characterization of the molecular mechanism of their action, including recent advances in the field, are reviewed in this article.

  1. The Heme Sensor System of Staphylococcus aureus

    OpenAIRE

    Stauff, Devin L; Skaar, Eric P.

    2009-01-01

    The important human pathogen Staphylococcus aureus is able to satisfy its nutrient iron requirement by acquiring heme from host hemoglobin in the context of infection. However, heme acquisition exposes S. aureus to heme toxicity. In order to detect the presence of toxic levels of exogenous heme, S. aureus is able to sense heme through the heme sensing system (HssRS) two-component system. Upon sensing heme, HssRS directly regulates the expression of the heme-regulated ABC transporter HrtAB, wh...

  2. Staphylococcus aureus infections in psittacine birds.

    Science.gov (United States)

    Hermans, K; Devriese, L A; De Herdt, P; Godard, C; Haesebrouck, F

    2000-10-01

    Staphylococcus aureus was isolated from internal organs of 13 different psittacine birds submitted for necropsy over a period of 6 years. The birds all had lesions consistent with septicaemia. S. aureus isolates included three different phage types. In seven of the 13 birds, concurrent infections with Chlamydophila species, Enterococcus hirae, Candida species, unidentified streptococci and coagulasenegative staphylococci were detected. One bird also had lesions of lymphoid leucosis. Few indications were found that staphylococcosis associated problems may spread epidemically. The present studies suggest that S. aureus is pathogenic for psittacine birds, although it does not seem to be a frequent cause of disease. PMID:19184832

  3. Opportunistic scheduling policies for improved throughput guarantees in wireless networks

    Directory of Open Access Journals (Sweden)

    Hassel Vegard

    2011-01-01

    Full Text Available Abstract Offering throughput guarantees for cellular wireless networks, carrying real-time traffic, is of interest to both the network operators and the customers. In this article, we formulate an optimization problem which aims at maximizing the throughput that can be guaranteed to the mobile users. By building on results obtained by Borst and Whiting and by assuming that the distributions of the users' carrier-to-noise ratios are known, we find the solution to this problem for users with different channel quality distributions, for both the scenario where all the users have the same throughput guarantees, and the scenario where all the users have different throughput guarantees. Based on these solutions, we also propose two simple and low complexity adaptive scheduling algorithms that perform significantly better than other well-known scheduling algorithms. We further develop an expression for the approximate throughput guarantee violation probability for users in time-slotted networks with the given cumulants of the distribution of bit-rate in a time-slot, and a given distribution for the number of time-slots allocated within a time-window.

  4. LEGAL GUARANTEES OF FARMERS’ TRADE UNIONS’ FUNCTIONING IN POLAND

    Directory of Open Access Journals (Sweden)

    Jakub Gołaś

    2016-03-01

    Full Text Available The main purpose of the article is to present legal basis and guarantees of the farmers’ trade unions’ functioning in Poland. The presented research was based on a thorough examination of national legal acts, which constitute both legal background and foundation of farmer’s unions’ activities and internal structure. The author tries to introduce both the individual legal and system guarantees which are dedicated to farmers’ organization and which exact acts shall be considered as the direct legal basis of the guarantees. Having regard to the fact that even the Polish Constitution introduces transparent and direct legal frameworks for functioning of farmers’ trade unions, the author intends to prove that individual farmers’ unions’ operations should be considered as a fundamental and basic feature of the national agricultural system. The presented work should be also considered as thorough examination of legal provisions presented in the Act on individual farmers’ trade unions of 7th April 1989 (Ustawa…, 2015b, which is direct legal basis for functioning guarantees of the agricultural unions. The author shows how the Act is governing such fundamental issues as creation, registration, liquidation or legal operational guarantees of each individual farmers’ trade unions operating under the Act.

  5. Laboratory Maintenance of Methicillin-Resistant Staphylococcus aureus (MRSA)

    OpenAIRE

    Nicholas P Vitko; Richardson, Anthony R.

    2013-01-01

    Staphylococcus aureus is an important bacterial pathogen in the hospital and community settings, especially Staphylococcus aureus clones that exhibit methicillin-resistance (MRSA). Many strains of S. aureus are utilized in the laboratory, underscoring the genetic differences inherent in clinical isolates. S. aureus grows quickly at 37°C with aeration in rich media (e.g. BHI) and exhibits a preference for glycolytic carbon sources. Furthermore, S. aureus has a gold pigmentation, exhibits β-hem...

  6. Unpower aerocraft augmented state feedback tracking guaranteed cost control

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Aimed at designing the unpower aerocraft attitude control system in a simple and practical way,the guaranteed cost control is adopted.To eliminate the steady-error,a novel tracking control approach-augmented state feedback tracking guaranteed cost control is proposed.Firstly,the unpower aerocraft is modeled as a linear system with norm bounded parameter uncertain,then the linear matrix inequality based state feedback gnaranteed cost control law is combined with the augmented state feedback tracking control from a new point of view.The sufficient condition of the existence of the augmented state feedback tracking guaranteed cost control is derived and converted to the feasible problem of the linear matrix inequality.Finally,the proposed approach is applied to a specified unpower aerocraft.The six dimensions of freedom simulation results show that the proposed approach is effective and feasible.

  7. The Heme Sensor System of Staphylococcus aureus

    Science.gov (United States)

    Stauff, Devin L.; Skaar, Eric P.

    2016-01-01

    The important human pathogen Staphylococcus aureus is able to satisfy its nutrient iron requirement by acquiring heme from host hemoglobin in the context of infection. However, heme acquisition exposes S. aureus to heme toxicity. In order to detect the presence of toxic levels of exogenous heme, S. aureus is able to sense heme through the heme sensing system (HssRS) two-component system. Upon sensing heme, HssRS directly regulates the expression of the heme-regulated ABC transporter HrtAB, which alleviates heme toxicity. Importantly, the inability to sense or respond to heme alters the virulence of S. aureus, highlighting the importance of heme sensing and detoxification to staphylococcal pathogenesis. Furthermore, potential orthologues of the Hss and Hrt systems are found in many species of Gram-positive bacteria, a possible indication that heme stress is a challenge faced by bacteria whose habitats include host tissues rich in heme. PMID:19494582

  8. Binding of heparan sulfate to Staphylococcus aureus.

    OpenAIRE

    Liang, O D; Ascencio, F; Fransson, L A; Wadström, T

    1992-01-01

    Heparan sulfate binds to proteins present on the surface of Staphylococcus aureus cells. Binding of 125I-heparan sulfate to S. aureus was time dependent, saturable, and influenced by pH and ionic strength, and cell-bound 125I-heparan sulfate was displaced by unlabelled heparan sulfate or heparin. Other glycosaminoglycans of comparable size (chondroitin sulfate and dermatan sulfate), highly glycosylated glycoprotein (hog gastric mucin), and some anionic polysaccharides (dextran sulfate and RNA...

  9. The Staphylococcus aureus “superbug”

    OpenAIRE

    FOSTER, TIMOTHY JAMES

    2004-01-01

    PUBLISHED There has been some debate about the disease-invoking potential of Staphylococcus aureus strains and whether invasive disease is associated with particularly virulent genotypes, or "superbugs." A study in this issue of the JCI describes the genotyping of a large collection of nonclinical, commensal S. aureus strains from healthy individuals in a Dutch population. Extensive study of their genetic relatedness by amplified restriction fragment typing and comparison with strains that...

  10. Transmissible mupirocin resistance in Staphylococcus aureus.

    OpenAIRE

    Rahman, M.; Noble, W. C.; Cookson, B

    1989-01-01

    The spread of two strains of Staphylococcus aureus with high level resistance to mupirocin is described. The resistance proved to be easily transferred to other S. aureus strains by filter mating experiments and on the skin of mice. No plasmid band corresponding to the resistance could be demonstrated by agarose gel electrophoresis or by caesium chloride gradient centrifugation but cleavage of 'chromosomal' DNA from resistant recipients showed bright bands of DNA absent from sensitive controls.

  11. Triclosan Promotes Staphylococcus aureus Nasal Colonization

    OpenAIRE

    Syed, Adnan K.; Ghosh, Sudeshna; Love, Nancy G.; Boles, Blaise R.

    2014-01-01

    ABSTRACT The biocide triclosan is used in many personal care products, including toothpastes, soaps, clothing, and medical equipment. Consequently, it is present as a contaminant in the environment and has been detected in some human fluids, including serum, urine, and milk. Staphylococcus aureus is an opportunistic pathogen that colonizes the noses and throats of approximately 30% of the population. Colonization with S. aureus is known to be a risk factor for several types of infection. Here...

  12. PARAMETER KINETIKA INAKTIVASI TERMAL DAN ISOLASI Staphylococcus aureus PADA MINUMAN DARI GEL CINCAU HIJAU DAN ROSELA [Thermal Inactivation Kinetics Parameter and Isolation of Staphylococcus aureus on Drink from Green Grass Jelly and Roselle

    Directory of Open Access Journals (Sweden)

    Eko Hari Purnomo

    2015-07-01

    Full Text Available Information about heat resistance (D and z values of target bacteria is needed for the thermal process design on drink from Green Grass Jelly (Premna oblongifolia Merr. and Roselle (Hibiscus sabdariffa L., so it can guarantee quality and safety of the product. The objectives of this research were to isolate Staphylococcus aureus from commercial green grass jelly and to determine the D and z values of Staphylococcus aureus (from commercial product and standard clinical isolate ATCC 25923 on heating menstruum of green grass jelly and roselle. Isolation of S. aureus was done by inoculation in selective medium, D and z values of Staphylococcus aureus were assessed by heating at constant temperatures of 57, 53, 49, and 45°C during the time interval 2.5, 5, 10, and 15 minutes. The results showed that one isolate (Isolate A gave positive response of Staphylococcus aureus in isolation tests and had similar percentage with the reference culture of 41.8% using the API Staph Kit. Heat resistance of Staphylococcus aureus (represented as D value isolated from green grass jelly at constant heating temperature of D45, D49, D53 and D57 were 32.3, 17.9, 4.6, and 1.5 minutes. On the other hand, D value of isolates ATCC 25923 (standard clinical isolate at constant heating temperature of D45, D49, D53 and D57 were 18.5, 6.8, 2.9, and 1.4 minutes. The z value of isolates A and ATCC 25923 were 8.8°C and 10.8°C. Smaller z value of isolate A showed that pasteurization process can be accelerated and optimized with increasing the temperature slightly but has the same lethality effect.

  13. Calculation of guaranteed mean power from wind turbine generators

    Science.gov (United States)

    Spera, D. A.

    1981-01-01

    A method for calculating the 'guaranteed mean' power output of a wind turbine generator is proposed. The term 'mean power' refers to the average power generated at specified wind speeds during short-term tests. Correlation of anemometers, the method of bins for analyzing non-steady data, the PROP Code for predicting turbine power, and statistical analysis of deviations in test data from theory are discussed. Guaranteed mean power density for the Clayton Mod-OA system was found to be 8 watts per square meter less than theoretical power density at all power levels, with a confidence level of 0.999. This amounts to 4 percent of rated power.

  14. Guarantees of Augmented Trace Norm Models in Tensor Recovery

    OpenAIRE

    Shi, Ziqiang; Han, Jiqing; Zheng, Tieran; Deng, Shiwen; Li, Ji

    2012-01-01

    This paper studies the recovery guarantees of the models of minimizing $\\|\\mathcal{X}\\|_*+\\frac{1}{2\\alpha}\\|\\mathcal{X}\\|_F^2$ where $\\mathcal{X}$ is a tensor and $\\|\\mathcal{X}\\|_*$ and $\\|\\mathcal{X}\\|_F$ are the trace and Frobenius norm of respectively. We show that they can efficiently recover low-rank tensors. In particular, they enjoy exact guarantees similar to those known for minimizing $\\|\\mathcal{X}\\|_*$ under the conditions on the sensing operator such as its null-space property, ...

  15. Robust levitation control for maglev systems with guaranteed bounded airgap.

    Science.gov (United States)

    Xu, Jinquan; Chen, Ye-Hwa; Guo, Hong

    2015-11-01

    The robust control design problem for the levitation control of a nonlinear uncertain maglev system is considered. The uncertainty is (possibly) fast time-varying. The system has magnitude limitation on the airgap between the suspended chassis and the guideway in order to prevent undesirable contact. Furthermore, the (global) matching condition is not satisfied. After a three-step state transformation, a robust control scheme for the maglev vehicle is proposed, which is able to guarantee the uniform boundedness and uniform ultimate boundedness of the system, regardless of the uncertainty. The magnitude limitation of the airgap is guaranteed, regardless of the uncertainty. PMID:26524957

  16. Independence:Guarantee to Emerson’s Individualism

    Institute of Scientific and Technical Information of China (English)

    Jiang; Jingxin

    2015-01-01

    Emerson established a systematic philosophy of individualism at his early stage.The basic three elements of individualism described in Emerson’s works are:freedom,selfidentity and independence to others.Independence,serves as the guarantee to the realization of individualism.

  17. Efficient Strategy-Proof Exchange and Minimum Consumption Guarantees

    OpenAIRE

    Shigehiro Serizawa; Weymark, John A.

    2002-01-01

    For exchange economies with classical economic preferences, it is shown that any strategy-proof social choice function that selects Pareto optimal outcomes cannot guarantee everyone a consumption bundle bounded away from the origin. This result demonstrates that there is a fundamental conflict between efficiency and distributional goals in exchange economies if the social choice rule is required to be strategy-proof.

  18. 76 FR 13769 - Rural Broadband Access Loans and Loan Guarantees

    Science.gov (United States)

    2011-03-14

    ... for low- and high-end service jobs, from call centers to software development, but does not guarantee... characteristics, suggesting that policy, economic, and social factors can overcome common barriers to broadband... especially significant given the importance of ] ongoing parental involvement in children's...

  19. 13 CFR 115.30 - Submission of Surety's guarantee application.

    Science.gov (United States)

    2010-01-01

    ... days, or in the case of a bonding line, within 15 business days (see § 115.33(d)(2)) after Execution of... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Submission of Surety's guarantee application. 115.30 Section 115.30 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SURETY...

  20. 78 FR 48135 - Information Collection; Guaranteed Farm Loan Program

    Science.gov (United States)

    2013-08-07

    ... online instructions for submitting comments. Mail: Trent Rogers, Senior Loan Specialist, USDA, FSA, Stop... above addresses. FOR FURTHER INFORMATION CONTACT: Trent Rogers, Senior Loan Specialist, (202) 720-3889...; ] DEPARTMENT OF AGRICULTURE Farm Service Agency Information Collection; Guaranteed Farm Loan Program...

  1. 76 FR 57940 - CCC Export Credit Guarantee (GSM-102) Program

    Science.gov (United States)

    2011-09-19

    ...) Program'' in the Federal Register (76 FR 44836-44855). The GSM-102 Program is administered by the Foreign...) published a proposed rule on July 27, 2011 (76 FR 44836-44855), revising and amending the regulations that... Guarantee (GSM-102) Program AGENCY: Foreign Agricultural Service and Commodity Credit Corporation,...

  2. 12 CFR 614.4800 - Guarantees and contracts of suretyship.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Guarantees and contracts of suretyship. 614.4800 Section 614.4800 Banks and Banking FARM CREDIT ADMINISTRATION FARM CREDIT SYSTEM LOAN POLICIES AND... another, or otherwise become a guarantor if an eligible cooperative substantially benefits from...

  3. Improvements Needed in Administration of the Guaranteed Student Loan Program.

    Science.gov (United States)

    General Accounting Office, Washington, DC.

    This report identifies improvements needed in administration of the guaranteed student loan program. Improvements needed are based on the fact that that lenders are not informed when student borrowers drop out of school. Alternatives for providing lenders with timely information on enrollment terminations are indicated. Additional administrative…

  4. Potassium Uptake Modulates Staphylococcus aureus Metabolism.

    Science.gov (United States)

    Gries, Casey M; Sadykov, Marat R; Bulock, Logan L; Chaudhari, Sujata S; Thomas, Vinai C; Bose, Jeffrey L; Bayles, Kenneth W

    2016-01-01

    As a leading cause of community-associated and nosocomial infections, Staphylococcus aureus requires sophisticated mechanisms that function to maintain cellular homeostasis in response to its exposure to changing environmental conditions. The adaptation to stress and maintenance of homeostasis depend largely on membrane activity, including supporting electrochemical gradients and synthesis of ATP. This is largely achieved through potassium (K(+)) transport, which plays an essential role in maintaining chemiosmotic homeostasis, affects antimicrobial resistance, and contributes to fitness in vivo. Here, we report that S. aureus Ktr-mediated K(+) uptake is necessary for maintaining cytoplasmic pH and the establishment of a proton motive force. Metabolite analyses revealed that K(+) deficiency affects both metabolic and energy states of S. aureus by impairing oxidative phosphorylation and directing carbon flux toward substrate-level phosphorylation. Taken together, these results underline the importance of K(+) uptake in maintaining essential components of S. aureus metabolism. IMPORTANCE Previous studies describing mechanisms for K(+) uptake in S. aureus revealed that the Ktr-mediated K(+) transport system was required for normal growth under alkaline conditions but not under neutral or acidic conditions. This work focuses on the effect of K(+) uptake on S. aureus metabolism, including intracellular pH and carbon flux, and is the first to utilize a pH-dependent green fluorescent protein (GFP) to measure S. aureus cytoplasmic pH. These studies highlight the role of K(+) uptake in supporting proton efflux under alkaline conditions and uncover a critical role for K(+) uptake in establishing efficient carbon utilization. PMID:27340697

  5. Intra-cellular Staphylococcus aureus alone causes infection in vivo

    Directory of Open Access Journals (Sweden)

    T Hamza

    2013-07-01

    Full Text Available Chronic and recurrent bone infections occur frequently but have not been explained. Staphylococcus aureus (S. aureus is often found among chronic and recurrent infections and may be responsible for such infections. One possible reason is that S. aureus can internalize and survive within host cells and by doing so, S. aureus can evade both host defense mechanisms and most conventional antibiotic treatments. In this study, we hypothesized that intra-cellular S. aureus could induce infections in vivo. Osteoblasts were infected with S. aureus and, after eliminating extra-cellular S. aureus, inoculated into an open fracture rat model. Bacterial cultures and radiographic observations at post-operative day 21 confirmed local bone infections in animals inoculated with intra-cellular S. aureus within osteoblasts alone. We present direct in vivo evidence that intra-cellular S. aureus could be sufficient to induce bone infection in animals; we found that intra-cellular S. aureus inoculation of as low as 102 colony forming units could induce severe bone infections. Our data may suggest that intra-cellular S. aureus can “hide” in host cells during symptom-free periods and, under certain conditions, they may escape and lead to infection recurrence. Intra-cellular S. aureus therefore could play an important role in the pathogenesis of S. aureus infections, especially those chronic and recurrent infections in which disease episodes may be separated by weeks, months, or even years.

  6. Antimicrobial (Drug) Resistance: Methicillin-Resistant Staphylococcus aureus (MRSA)

    Science.gov (United States)

    ... Marketing Share this: Main Content Area Methicillin-Resistant Staphylococcus aureus (MRSA) During the past four decades, methicillin-resistant Staphylococcus aureus , or MRSA, has evolved from a controllable ...

  7. Evolution of methicillin-resistant Staphylococcus aureus towards increasing resistance

    DEFF Research Database (Denmark)

    Strommenger, Birgit; Bartels, Mette Damkjær; Kurt, Kevin;

    2014-01-01

    To elucidate the evolutionary history of Staphylococcus aureus clonal complex (CC) 8, which encompasses several globally distributed epidemic lineages, including hospital-associated methicillin-resistant S. aureus (MRSA) and the highly prevalent community-associated MRSA clone USA300....

  8. Classification of Healthcare-Associated Staphylococcus aureus Bacteremia

    DEFF Research Database (Denmark)

    Smit, Jesper; Søgaard, Mette; Schønheyder, Henrik Carl; Nielsen, Henrik; Thomsen, Reimar Wernich

    2016-01-01

    We investigated whether different definitions of healthcare-associated infection influenced the prevalence, characteristics, and mortality of patients with Staphylococcus aureus bacteremia. With different definitions, the proportion of patients classified as having healthcare-associated S. aureus...

  9. Comparative Efficacy of Ceftaroline with Linezolid against Staphylococcus Aureus and Methicillin Resistant Staphylococcus Aureus

    International Nuclear Information System (INIS)

    Objective:To compare the in vitro antimicrobial efficacy of ceftaroline with linezolid against Staphylococcus aureus and methicillin resistant Staphylococcus aureus. Study Design: Quasi-experimental study. Place and Duration of Study: Microbiology Department, Army Medical College, Rawalpindi, from January to December 2013. Methodology: Clinical samples from respiratory tract, blood, pus and various catheter tips routinely received in the Department of Microbiology, Army Medical College, Rawalpindi were innoculated on blood and MacConkey agar. Staphylococcus aureus was identified by colony morphology, Gram reaction, catalase test and coagulase test. Methicillin resistant Staphylococcus aureus detection was done by modified Kirby Bauer disc diffusion method using cefoxitin disc (30g) and the isolates were considered methicillin resistant if the zone of inhibition around cefoxitin disc was /sup 2/ 21 mm. Bacterial suspensions of 56 Staphylococcus aureus isolates and 50 MRSA isolates were prepared, which were standardized equal to 0.5 McFarland's turbidity standard and inoculated on Mueller-Hinton agar plates followed by application of ceftaroline and linezolid disc (Oxoid, UK), according to manufacturer's instructions. The plates were then incubated at 37 Degree C aerobically for 18 - 24 hours. Diameters of inhibition zone were measured and interpretated as per Clinical and Laboratory Standards Institute (CLSI) guidelines. Results: Out of 106 isolates all of the 56 Staphylococcus aureus (100%) were sensitive to ceftaroline and linezolid. However, out of 50 methicillin resistant Staphylococcus aureus, 48 (96%) were sensitive to ceftaroline whereas, 49 (98%) were sensitive to linezolid. Conclusion: Ceftaroline is equally effective as linezolid against Staphylococcus aureus and methicillin resistant Staphylococcus aureus. (author)

  10. Mobile intelligent agent entity model towards QoS guarantee

    Institute of Scientific and Technical Information of China (English)

    LI Jie; WANG Ru-chuan; BIAN Zhen-gai

    2006-01-01

    Implementing a flexible configuration of the QoS parameter in a distributed computing network has become a problem due to the weak scalability of current approaches.In an effort to solve this problem,an inner basic model of an intelligent agent (IA) is presented.The IA functionality was extended by introducing a primarily mobile agent.A QoS guarantee scheme was subsequently designed and implemented based on the model as well.By utilizing the proposed scheme,the IA can sense,predict and configure the data flow traffic.Since the communicating ability was considered and provided,the competition among different devices could be eliminated effectively and the global traffic can be optimized.The results of the simulations have shown that the proposed model can provide a QoS guarantee.

  11. SHALE: An Efficient Algorithm for Allocation of Guaranteed Display Advertising

    CERN Document Server

    Bharadwaj, Vijay; Ma, Wenjing; Nagarajan, Chandrashekhar; Tomlin, John; Vassilvitskii, Sergei; Vee, Erik; Yang, Jian

    2012-01-01

    Motivated by the problem of optimizing allocation in guaranteed display advertising, we develop an efficient, lightweight method of generating a compact {\\em allocation plan} that can be used to guide ad server decisions. The plan itself uses just O(1) state per guaranteed contract, is robust to noise, and allows us to serve (provably) nearly optimally. The optimization method we develop is scalable, with a small in-memory footprint, and working in linear time per iteration. It is also "stop-anytime", meaning that time-critical applications can stop early and still get a good serving solution. Thus, it is particularly useful for optimizing the large problems arising in the context of display advertising. We demonstrate the effectiveness of our algorithm using actual Yahoo! data.

  12. Automated Assume-Guarantee Reasoning by Abstraction Refinement

    Science.gov (United States)

    Pasareanu, Corina S.; Giannakopoulous, Dimitra; Glannakopoulou, Dimitra

    2008-01-01

    Current automated approaches for compositional model checking in the assume-guarantee style are based on learning of assumptions as deterministic automata. We propose an alternative approach based on abstraction refinement. Our new method computes the assumptions for the assume-guarantee rules as conservative and not necessarily deterministic abstractions of some of the components, and refines those abstractions using counter-examples obtained from model checking them together with the other components. Our approach also exploits the alphabets of the interfaces between components and performs iterative refinement of those alphabets as well as of the abstractions. We show experimentally that our preliminary implementation of the proposed alternative achieves similar or better performance than a previous learning-based implementation.

  13. To help allies, send security guarantees, not nuclear bombs

    OpenAIRE

    Fuhrmann, Matthew; Sechser , Todd S.

    2014-01-01

    Despite the end of the Cold War more than two decades ago, the U.S. still deploys nuclear weapons in several countries. But does deploying these weapons act as a deterrent to conflict, making these countries safer? In new research which analyses the effects of such nuclear deployments, Matthew Fuhrmann and Todd S. Sechser find that their presence does little, when compared with alliance guarantees. They write that having an alliance with a nuclear armed ally is actually more effective in prev...

  14. Information Extraction on the Web with Credibility Guarantee

    OpenAIRE

    Nguyen, Thanh Tam

    2015-01-01

    The Web became the central medium for valuable sources of information extraction applications. However, such user-generated resources are often plagued by inaccuracies and misinformation due to the inherent openness and uncertainty of the Web. In this work we study the problem of extracting structured information out of Web data with a credibility guarantee. The ultimate goal is that not only the structured information should be extracted as much as possible but also its credibility is high. ...

  15. Traffic Scheduling in WDM Passive Optical Network with Delay Guarantee

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    WDM passive optical network becomes more favorable as the required bandwidth increases, but currently few media access control algorithms adapted to WDM access network. This paper presented a new scheduling algorithm for bandwidth sharing in WDM passive optical networks, which provides per-flow delay guarantee and supports variable-length packets scheduling. Through theoretical analysis and simulation, the end-to-end delay bound and throughput fairness of the algorithm was demonstrated.

  16. Organic Law Of Judicial Guarantees And Constitutional Control

    OpenAIRE

    Ernesto López Freire

    2013-01-01

    This paper demonstrates the various unconstitutional and fallacies of the Organic Law of Judicial guarantees and Constitutional Control. For that, there will be a comprehensive collation between the mentioned law and the Constitution of the Republic of Ecuador and force. Through this analysis shows a lack of knowledge of Ecuadorian law or legal science by their authors. This study elucidated, inter alia, the inconsistencies in matters relating to the interpretation of constitutional provision...

  17. Money-Back Guarantees: Helping the Low-Quality Retailer

    OpenAIRE

    Bruce McWilliams

    2012-01-01

    Existing literature, based on signaling theory, suggests that money-back guarantees (MBGs) will be utilized by high-quality firms, where high quality is defined as a low likelihood of product return. However, in today's world, MBGs are ubiquitous among major retailers, even when the likelihood of product return varies greatly between them. To understand this phenomenon, we explore a competitive environment between high- and low-quality retailers where consumers are fully informed and risk neu...

  18. GEARSHIFT: Guaranteeing availability requirements in SLAs using hybrid fault tolerance

    OpenAIRE

    Gonzalez, Andres Javier; Helvik, Bjarne Emil; Tiwari, Prakriti; Denis, Becker; Wittner, Otto Jonassen

    2015-01-01

    The dependability of ICT systems is vital for today's society. However, operational systems are not fault free. Providers and customers have to define clear availability requirements and penalties on the delivered services by using SLAs. Fulfilling the stipulated availability may be expensive. The lack of mechanisms that allow a fine control of the SLA risk may lead to over-dimension the provided resources. Therefore, a relevant question for ICT service providers is: How to guarantee the SLA ...

  19. Providing Credit Guarantee Scheme in Agriculture of Ukraine

    OpenAIRE

    Olena Oliynyk

    2013-01-01

    Small and medium-size businesses, despite their significant contribution to agricultural production in Ukraine, do have limited access to financing. Existing public support mechanisms (interest rate subsidies, leasing programs) do not solve the problem of these categories of enterprises access to credit resources. Introduction of credit guarantee scheme is a more effective mechanism to support small and medium-size businesses. As international experience shows, it is a market mechanism - the ...

  20. Distributed Model Predictive Control with Suboptimality and Stability Guarantees

    OpenAIRE

    Giselsson, Pontus; Rantzer, Anders

    2010-01-01

    Theory for Distributed Model Predictive Control (DMPC) is developed based on dual decomposition of the convex optimization problem that is solved in each time sample. The process to be controlled is an interconnection of several subsystems, where each subsystem corresponds to a node in a graph. We present a stopping criterion for the DMPC scheme that can be locally verified by each node and that guarantees closed loop suboptimality above a pre-specified level and asymptotic stability of the i...

  1. STF: PROVIDE RATE GUARANTEES IN INPUT-QUEUED SWITCH

    Institute of Scientific and Technical Information of China (English)

    Wang Wenjie; Wei Gang

    2002-01-01

    A QoS-aware input-queued scheduling algorithm, called Smallest Timestamp First (STF), is proposed, which is improved upon iSLIP and can allocate bandwidth among inputs sharing a common output based on their reservation by assigning suitable finishing timestamps to contending cells. STF can also provide isolation between flows that share a common output link.Misbehaving flows will be restricted to guarantee the behaving flows' bandwidth. Simulatious prove the feasibility of our algorithm.

  2. Guaranteed Minimum Price Contracts for Some, an Insurance for Others?

    OpenAIRE

    Chambolle, Claire; Poret, Sylvaine,

    2008-01-01

    This paper analyzes the impact of guaranteed minimum price contracts between sub-groups of farmers and a fair trade manufacturer on the spot market price. We focus on the fair trade concept in the coffee supply chain as an example. We analyze a three level vertical chain gathering perfectly competitive farmers upstream who offer their raw product to manufacturers who then sell finished products to a downstream retailer. Without fair trade, all the raw product is sold on the spot market. When ...

  3. Methods For Guaranteeing Contracted Availability In Connection Oriented Networks

    OpenAIRE

    Gonzalez Martinez, Andres Javier

    2013-01-01

    Real telecommunications networks are not failure free. Any single disconnection impacts the network provider reputation and finances, and produces incalculable consequences to the customers through the affected applications. A common policy to handle this issue is the stipulation of the availability to be guaranteed in a business contract known as Service Level Agreement SLA. The stipulated availability must be commercially competitive, and it must fit the customer needs. However, the fulfill...

  4. Characterization of a Mouse-Adapted Staphylococcus aureus Strain

    OpenAIRE

    Holtfreter, Silva; Fiona J Radcliff; Grumann, Dorothee; Read, Hannah; Johnson, Sarah; Monecke, Stefan; Ritchie, Stephen; Clow, Fiona; Goerke, Christiane; Bröker, Barbara M.; Fraser, John D.; Wiles, Siouxsie

    2013-01-01

    More effective antibiotics and a protective vaccine are desperately needed to combat the ‘superbug’ Staphylococcus aureus. While in vivo pathogenicity studies routinely involve infection of mice with human S. aureus isolates, recent genetic studies have demonstrated that S. aureus lineages are largely host-specific. The use of such animal-adapted S. aureus strains may therefore be a promising approach for developing more clinically relevant animal infection models. We have isolated a mouse-ad...

  5. Meticillin-resistant Staphylococcus aureus (MRSA)

    DEFF Research Database (Denmark)

    Stefani, Stefania; Chung, Doo Ryeon; Lindsay, Jodi A;

    2012-01-01

    This article reviews recent findings on the global epidemiology of healthcare-acquired/associated (HA), community-acquired/associated (CA) and livestock-associated (LA) meticillin-resistant Staphylococcus aureus (MRSA) and aims to reach a consensus regarding the harmonisation of typing methods...... health. Continuous efforts to understand the changing epidemiology of S. aureus infection in humans and animals are therefore necessary, not only for appropriate antimicrobial treatment and effective infection control but also to monitor the evolution of the species. The group made several consensus...

  6. A sensitive assay for Staphylococcus aureus nucleases

    International Nuclear Information System (INIS)

    A sensitive assay for staphylococcal nuclease involving incubation of the enzyme sample with heat-denatured [3H] thymidine labelled DNA from E.coli, precipitation with trichloroacetic acid and measurement of the radioactivity of acid-soluble nucleotides released has been developed. The assay is sensitive enough to be used for comparing the levels of nucleases elaborated by different strains of S. aureus as well as for determining the extent of contamination of S. aureus in food and water samples even at levels at which the conventional spectrophotometric and toluidine blue-DNA methods are totally inadequate. (author). 26 refs., 3 figs ., 3 tabs

  7. Staphylococcus aureus bacteremia in hemodialysis patients.

    Science.gov (United States)

    Latos, D L; Stone, W J; Alford, R H

    1977-01-01

    Fifteen male hemodialysis patients developed 21 episodes of S. aureus bacteremia. Infections involving vascular access were responsible for 65% of initial bacteremias. The arteriovenous fistula was the most prevalent type of access used, and thus was responsible for the majority of these illnesses. Phage typing indicated that recurrent episodes were due to reinfection rather than relapse. Complications included endocarditis, osteomyelitis, septic embolism, and pericarditis. One patient died of infectious complications. It is recommended that hemodialysis patients developing bacteremia due to S. aureus receive at least 6 weeks of beta lactamase-resistant antimicrobial therapy. PMID:608860

  8. Assume-Guarantee Synthesis for Digital Contract Signing

    CERN Document Server

    Chatterjee, Krishnendu

    2010-01-01

    We study the automatic synthesis of fair non-repudiation protocols, a class of fair exchange protocols, used for digital contract signing. First, we show how to specify the objectives of the participating agents and the trusted third party (TTP) as path formulas in LTL and prove that the satisfaction of these objectives imply fairness and abuse-freeness; properties required of fair exchange protocols. We then show that weak (co-operative) co-synthesis and classical (strictly competitive) co-synthesis fail, whereas assume-guarantee synthesis (AGS) succeeds. We demonstrate the success of assume-guarantee synthesis as follows: (a) any solution of assume-guarantee synthesis is attack-free; no subset of participants can violate the objectives of the other participants; (b) the Asokan-Shoup-Waidner (ASW) certified mail protocol that has known vulnerabilities is not a solution of AGS; (c) The Garay-Jakobsson-MacKenzie (GJM) protocol, while fair and abuse-free, is not attack-free by our definition and is hence not a ...

  9. 21 CFR 866.3700 - Staphylococcus aureus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Staphylococcus aureus serological reagents. 866... Staphylococcus aureus serological reagents. (a) Identification. Staphylococcus aureus serological reagents are... diagnosis of disease caused by this bacterium belonging to the genus Staphylococcus and...

  10. 9 CFR 113.115 - Staphylococcus Aureus Bacterin-Toxoid.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Staphylococcus Aureus Bacterin-Toxoid... REQUIREMENTS Inactivated Bacterial Products § 113.115 Staphylococcus Aureus Bacterin-Toxoid. Staphylococcus... Staphylococcus aureus which has been inactivated and is nontoxic. Each serial of biological product...

  11. A pig model of acute Staphylococcus aureus induced pyemia

    DEFF Research Database (Denmark)

    Nielsen, O. L.; Iburg, T.; Aalbæk, B.;

    2009-01-01

    Background: Sepsis caused by Staphylococcus aureus constitutes an important cause of morbidity and mortality in humans, and the incidence of this disease-entity is increasing. In this paper we describe the initial microbial dynamics and lesions in pigs experimentally infected with S. aureus....... aureus isolated from man and an extension of the timeframe aiming at inducing sepsis, severe sepsis and septic shock....

  12. Multidrug-Resistant Staphylococcus aureus in US Meat and Poultry

    OpenAIRE

    Waters, Andrew E.; Contente-Cuomo, Tania; Buchhagen, Jordan; Liu, Cindy M.; Watson, Lindsey; Pearce, Kimberly; Foster, Jeffrey T.; Bowers, Jolene; Driebe, Elizabeth M; Engelthaler, David M.; Keim, Paul S; Lance B Price

    2011-01-01

    We characterized the prevalence, antibiotic susceptibility profiles, and genotypes of Staphylococcus aureus among US meat and poultry samples (n = 136). S. aureus contaminated 47% of samples, and multidrug resistance was common among isolates (52%). S. aureus genotypes and resistance profiles differed significantly among sample types, suggesting food animal–specific contamination.

  13. Risk factors for Staphylococcus aureus and methicillin-resistant S aureus colonization among health care workers in pediatrics departments.

    Science.gov (United States)

    Gomes, Ivete Martins; Marlow, Mariel Asbury; Pinheiro, Marcos Gabriel; de Freitas, Maria de Fátima Nogueira; Fonseca, Fernanda Fernandes; Cardoso, Claudete Aparecida Araújo; Aguiar-Alves, Fábio

    2014-08-01

    Risk factors for Staphylococcus aureus and methicillin-resistant S aureus (MRSA) were evaluated for 178 health care workers from a public hospital pediatrics department in Brazil. Colonization rates were 33.1% for S aureus and 5.1% for MRSA. Risk factors for S aureus colonization differed from those for MRSA. Results suggest nurses with prolonged pediatric patient contact in inpatient units are at higher risk for MRSA colonization. PMID:25087145

  14. Common R-plasmids in Staphylococcus aureus and Staphylococcus epidermidis during a nosocomial Staphylococcus aureus outbreak.

    OpenAIRE

    Cohen, M. L.; Wong, E. S.; Falkow, S

    1982-01-01

    During a 7-month period in 1978 to 1979, 31 patients and personnel at a Kentucky hospital were colonized or infected with a Staphylococcus aureus strain resistant to clindamycin, erythromycin, gentamicin, methicillin, penicillin, and tetracycline. S. epidermidis with similar antibiotic resistance patterns had been isolated in this hospital in the year before the S. aureus outbreak. A 32-megadalton R-plasmid, pUW3626, mediating resistance to penicillin and gentamicin, was present in these isol...

  15. Staphylococcus aureus resistente a la meticilina (SARM)

    Centers for Disease Control (CDC) Podcasts

    2007-10-22

    Datos importantes sobre las infecciones por SARM en Estados Unidos, en las escuelas y los entornos médicos. (Title: Methicillin-resistant Staphylococcus aureus (MRSA)Created: 10/2007).  Created: 10/22/2007 by National Center for Preparedness, Detection, and Control of Infectious Diseases.   Date Released: 11/9/2007.

  16. Staphylococcus aureus enterotoxins A- and B

    DEFF Research Database (Denmark)

    Danielsen, E Michael; Hansen, Gert H; Karlsdóttir, Edda

    2013-01-01

    Enterotoxins of Staphylococcus aureus are among the most common causes of food poisoning. Acting as superantigens they intoxicate the organism by causing a massive uncontrolled T cell activation that ultimately may lead to toxic shock and death. In contrast to our detailed knowledge regarding...

  17. Staphylococcus aureus vaccines: Deviating from the carol.

    Science.gov (United States)

    Missiakas, Dominique; Schneewind, Olaf

    2016-08-22

    Staphylococcus aureus, a commensal of the human nasopharynx and skin, also causes invasive disease, most frequently skin and soft tissue infections. Invasive disease caused by drug-resistant strains, designated MRSA (methicillin-resistant S. aureus), is associated with failure of antibiotic therapy and elevated mortality. Here we review polysaccharide-conjugate and subunit vaccines that were designed to prevent S. aureus infection in patients at risk of bacteremia or surgical wound infection but failed to reach their clinical endpoints. We also discuss vaccines with ongoing trials for combinations of polysaccharide-conjugates and subunits. S. aureus colonization and invasive disease are not associated with the development of protective immune responses, which is attributable to a large spectrum of immune evasion factors. Two evasive strategies, assembly of protective fibrin shields via coagulases and protein A-mediated B cell superantigen activity, are discussed as possible vaccine targets. Although correlates for protective immunity are not yet known, opsonophagocytic killing of staphylococci by phagocytic cells offers opportunities to establish such criteria. PMID:27526714

  18. Carriage of Staphylococcus aureus in the elderly

    NARCIS (Netherlands)

    R. M. Parnaby; G. O'Dwyer; H. A. Monsey; M. S. Shafi

    1996-01-01

    textabstractThe point prevalence and incidence of Staphylococcus aureus (methicillin-sensitive and -resistant) carriage by inpatients on acute elderly care wards was estimated. The relationship to body site and to previous admissions to hospital or other institutions was determined. Fifty-five patie

  19. Increasing resistance of Staphylococcus aureus to ciprofloxacin.

    OpenAIRE

    Daum, T E; Schaberg, D R; Terpenning, M S; Sottile, W S; Kauffman, C A

    1990-01-01

    We demonstrated the marked emergence of resistance to ciprofloxacin among Staphylococcus arueus strains isolated at the Ann Arbor Veterans Administration Medical Center. All S. aureus isolates tested from 1984 to 1985 were susceptible, whereas 55.1% of methicillin-resistant and 2.5% of methicillin-susceptible strains from 1989 had high-level resistance to ciprofloxacin.

  20. Staphylococcus aureus spa type t437

    DEFF Research Database (Denmark)

    Glasner, C; Pluister, G; Westh, H;

    2015-01-01

    large epidemiological studies in Europe. Nevertheless, the overall numbers identified in some Northern European reference laboratories have increased during the past decade. To determine whether the S. aureus t437 clone is present in other European countries, and to assess its genetic diversity across...

  1. The Impact of a State Bond Guarantee on State Credit Markets and Individual Municipalities

    OpenAIRE

    Hsueh, L. Paul; Kidwell, David S.

    1988-01-01

    Examines the overall effect of a state bond guarantee on state credit markets and individual municipalities. Analyzes the Texas School Bond Guarantee program and it's impact on the borrowing cost of school districts and other municipalities within the state.

  2. Pricing of rate of return guarantees on multi-period assets

    OpenAIRE

    Lindset, Snorre

    2001-01-01

    The basis for this paper is the pricing of multi-period rate of return guarantees. These guarantees can typically be found in life insurance and pension contracts. We derive closed form solutions, expressed by the cumulative multivariate normal probability distribution, for multi-period rate of return guarantees on both a money market account and a stock. The guarantees of Hipp (1996), Persson and Aase (1997), and Miltersen and Persson (1999) can be seen to be special cases of our results.

  3. GUARANTEED COST CONTROL OF CONTINUOUS-TIME UNCERTAIN SINGULAR SYSTEM WITH BOTH STATE AND INPUT DELAYS

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    The guaranteed cost control problem for a continuous-time uncertain singular system with state and control delays, and a given quadratic cost function is studied in this paper. Sufficient conditions for the existence of the guaranteed cost controller are derived based on the linear inequality (LMI) approach. A parameterized characterization of the guaranteed cost laws is given in terms of the feasible solutions to a certain LMI, and the cost function of guaranteed cost controller exists an upper bound.

  4. Pricing rate of return guarantees in a Heath-Jarrow-Morton framework

    OpenAIRE

    Kristian R. Miltersen; Persson, Svein-Arne

    1998-01-01

    Rate of return guarantees are included in many financial products, for example life insurance contracts or guaranteed investment contracts issued by investment banks. The holder of such a contract is guaranteed a fixed periodically rate of return rather than - or in addition to - a fixed absolute amount at expiration. We consider rate of return guarantees where the underlying rate of return is either (i) the rate of return on a stock investment or (ii) the short-term interest rate. Various ty...

  5. Valuing Guaranteed Minimum Death Benefit Options in Variable Annuities Under a Benchmark Approach

    OpenAIRE

    Marquardt, T.; Eckhard Platen; S. Jaschke

    2008-01-01

    Variable annuities (VAs) represent a marked change from earlier life products in the guarantees that they offer and it is no longer possible to manage the risks of these liabilities using traditional actuarial methods. Thinking about guarantees as options suggests applying risk neutral pricing in order to value the embedded guarantees, such as guaranteed minimum death benefits (GMDBs). However, due to the long maturities of contracts, stochastic volatility and many other reasons, VA markets a...

  6. Relationship between Vancomycin-Resistant Staphylococcus aureus, Vancomycin-Intermediate S. aureus, High Vancomycin MIC, and Outcome in Serious S. aureus Infections

    OpenAIRE

    Holmes, Natasha E.; Johnson, Paul D. R.; Howden, Benjamin P.

    2012-01-01

    Vancomycin has been used successfully for over 50 years for the treatment of Staphylococcus aureus infections, particularly those involving methicillin-resistant S. aureus. It has proven remarkably reliable, but its efficacy is now being questioned with the emergence of strains of S. aureus that display heteroresistance, intermediate resistance, and, occasionally, complete vancomycin resistance. More recently, an association has been established between poor outcome and infections with strain...

  7. The Minimum Price Guarantee Policy before and after Real Plan

    Directory of Open Access Journals (Sweden)

    Jackelline Favro

    2014-12-01

    Full Text Available This study aims to analyze the performance of the Minimum Price Guarantee Policy - MPGPin Brazil for the main agricultural products, during the 1980s and 1990s, and especially the post-Real Plan period. The research has a bibliographic and descriptive nature. The main results showed that the MPGP remains an important tool for ensuring income to farmers and the supply of selected crops in the analyzed period, but there was a reduction of public action for the new MPGP and new private instruments has revealed efficiency

  8. Organic Law Of Judicial Guarantees And Constitutional Control

    Directory of Open Access Journals (Sweden)

    Ernesto López Freire

    2013-01-01

    Full Text Available This paper demonstrates the various unconstitutional and fallacies of the Organic Law of Judicial guarantees and Constitutional Control. For that, there will be a comprehensive collation between the mentioned law and the Constitution of the Republic of Ecuador and force. Through this analysis shows a lack of knowledge of Ecuadorian law or legal science by their authors. This study elucidated, inter alia, the inconsistencies in matters relating to the interpretation of constitutional provisions, full compensation, material and integral; challenge administrative acts, judicial unit.

  9. Routing Bandwidth Guaranteed Paths with Restoration in Label Switched Networks

    OpenAIRE

    Norden, Samphel; Buddhikot, Milind M.; Waldvogel, Marcel; Suri, Subhash

    2001-01-01

    A Network Service Provider (NSP) operating a label-switched networks such as ATM or Multi-Protocol Label Switching (MPLS) networks, sets up end-to-end bandwidth-guaranteed Label-Switched Paths (LSPs) to satisfy the connectivity requirements of its client networks. To make such a service highly available, the NSP may set up one or more backup LSPs for every active LSP. The backup LSPs are activated when the corresponding active LSP fails. Accordingly, the problem of LSP routing with and withou...

  10. Efficient Vessel Tracking  with Accuracy Guarantees

    DEFF Research Database (Denmark)

    Redoutey, Martin; Scotti, Eric; Jensen, Christian Søndergaard;

    2008-01-01

    vessels, transmits location-related information from vessels to ground stations that are part of a so-called Vessel Traffic Service (VTS), thus enabling these to track the movements of the vessels. This paper presents techniques that improve the existing AIS by offering better and guaranteed tracking...... accuracies at lower communication costs. The techniques employ movement predictions that are shared between vessels and the VTS. Empirical studies with a prototype implementation and real vessel data demonstrate that the techniques are capable of significantly improving the AIS....

  11. On guaranteed cost control of linear systems with input saturation

    Institute of Scientific and Technical Information of China (English)

    FU Min-yue

    2007-01-01

    This work studies the problem of control design for linear systems with input saturation. It is well known that integral quadratic constraints (IQC) can be used to describe input saturation and that the use of IQC in analysis can lead to less conservative performance bound and larger domain of attraction. In this work, it is shown that a class of commonly used IQCs may not help in control synthesis. That is, the use of these IQCs does not enlarge the guaranteed domain of performance for synthesis.

  12. 75 FR 21521 - Surety Bond Guarantee Program; Disaster and Miscellaneous Amendments

    Science.gov (United States)

    2010-04-26

    ... approve, under certain conditions, an SBA bond guarantee on an individual Contract or Order up to $5,000... services procured under a Federal Contract or Order up to $5,000,000, an SBA bond guarantee may be issued... efforts in the major disaster area. The SBA bond guarantee may be issued on a Federal Contract or...

  13. 7 CFR 457.128 - Guaranteed production plan of fresh market tomato crop insurance provisions.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Guaranteed production plan of fresh market tomato crop... § 457.128 Guaranteed production plan of fresh market tomato crop insurance provisions. The Guaranteed Production Plan of Fresh Market Tomato Crop Insurance FCIC Policies Department of Agriculture Federal...

  14. 7 CFR 1786.202 - Prepayment and refinancing of RUS guaranteed FFB loans.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 12 2010-01-01 2010-01-01 false Prepayment and refinancing of RUS guaranteed FFB...) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE (CONTINUED) PREPAYMENT OF RUS GUARANTEED AND INSURED LOANS TO ELECTRIC AND TELEPHONE BORROWERS Refinancing and Prepayment of RUS Guaranteed FFB...

  15. 7 CFR 1786.35 - Loss, theft, destruction, mutilation, or defacement of RUS guarantee.

    Science.gov (United States)

    2010-01-01

    ... of RUS guarantee. 1786.35 Section 1786.35 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE (CONTINUED) PREPAYMENT OF RUS GUARANTEED AND INSURED LOANS TO ELECTRIC AND TELEPHONE BORROWERS Prepayment of RUS Guaranteed Federal Financing...

  16. Insights into tRNA-Dependent Amidotransferase Evolution and Catalysis from the Structure of the Aquifex aeolicus Enzyme

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Jing; Bu, Weishu; Sheppard, Kelly; Kitabatake, Makoto; Kwon, Suk-Tae; Söll, Dieter; Smith, Janet L.; (Yale); (Michigan); (Kyoto)

    2010-08-17

    Many bacteria form Gln-tRNA{sup Gln} and Asn-tRNA{sup ASN} by conversion of the misacylated Glu-tRNA{sup Gln} and Asp-tRNA{sup ASN} species catalyzed by the GatCAB amidotransferase in the presence of ATP and an amide donor (glutamine or asparagine). Here, we report the crystal structures of GatCAB from the hyperthermophilic bacterium Aquifex aeolicus, complexed with glutamine, asparagine, aspartate, ADP, or ATP. In contrast to the Staphylococcus aureus GatCAB, the A. aeolicus enzyme formed acyl-enzyme intermediates with either glutamine or asparagine, in line with the equally facile use by the amidotransferase of these amino acids as amide donors in the transamidation reaction. A water-filled ammonia channel is open throughout the length of the A. aeolicus GatCAB from the GatA active site to the synthetase catalytic pocket in the B-subunit. A non-catalytic Zn{sup 2+} site in the A. aeolicus GatB stabilizes subunit contacts and the ammonia channel. Judged from sequence conservation in the known GatCAB sequences, the Zn{sup 2+} binding motif was likely present in the primordial GatB/E, but became lost in certain lineages (e.g., S. aureus GatB). Two divalent metal binding sites, one permanent and the other transient, are present in the catalytic pocket of the A. aeolicus GatB. The two sites enable GatCAB to first phosphorylate the misacylated tRNA substrate and then amidate the activated intermediate to form the cognate products, Gln-tRNA{sup Gln} or Asn-tRNA{sup ASN}.

  17. Staphylococcus aureus methicillin-resistance mechanisms

    Directory of Open Access Journals (Sweden)

    Petrović-Jeremić Ljiljana

    2008-01-01

    Full Text Available Background/Aim. In many hospitals in the world and in our country, the spread of methicillin-resistant Staphylococcus aureus (MRSA is so wide that nowdays vancomycin is recommended for empiric treatment of staphylococcal life threatening infections (sepsis, pneumonia instead of beta-lactam antibiotics. The aim of this study was to determine the production of beta-lactamases in hospital and community isolates of staphyloococus aureus, i. e. hospital associated MRSA (HA-MRSA and community associated MRSA (CA-MRSA, the presence of homogeneous and heterogeneous type of methicillin resistance, and border-line resistance in Staphylococcus aureus (BORSA. The aim of this study was also to determine if there was a statistically significant difference between mechanisms of resistance in HA-MRSA and CA-MRSA. Methods. A total 216 clinical Staphylococcus aureus isolates from the General Hospital in the town of Cuprija and 186 ambulance Staphylococcus aureus isolates from the community were examined for the presence of methicillin-resistance using disk-diffusion test with penicillin disk (10 ij, oxacillin disk (1 μg and cefoxitin disk (30 μg. Betalactamases production was detected by nitrocefin disk and betalactamase tablets. Determination of oxacillin minimum inhibitory concentracion (MIC was done by agar-dilution method. Results. The prevalence of HA-MRSA was 57.4%, and CA-MRSA was 17.7% (p < 0.05. There was a higher rate of heterogeneous type of resistance among clinical MRSA isolates (11.1% compared with ambulance ones (3.8% (p < 0.05. The rates of beta-lactamases production were similar among hospital associated isolates (97.5%, as well as in the community associated isolates (95.5% (p > 0.05. There were 4.6 % of BORSA hospital isolates and 3.3 % of BORSA ambulance isolates (p > 0.05. Conclusion. The frequency of MRSA isolates in hospital was significantly higher than in community, as well as the heterogeneous type of resistance. The frequency of BORSA

  18. DEPOSIT GUARANTEE SCHEMES JOIN FINANCIAL SAFETY-NET

    Directory of Open Access Journals (Sweden)

    Eugen Dijmarescu

    2011-03-01

    Full Text Available Deposit Guarantee Schemes (DGS become more visible under the current conditions of the world financial markets. If those have played in the past a rather discreet role for social stability through the function of pay box following a bank failure, the recent crisis put them in a new light, once the guarantee ceiling for eligible deposits grew in order to make for the accumulation of wealth worldwide and the increased risk incurred by the leverage of banking operations. Consequently, the resources accumulated by the DGS from the member banks and their proper management make possible their involvement in pre-emptive actions aimed to avoid bankruptcies via special administration and purchase of assets and assumption of liabilities. These new attributes give DGS a role in the mechanism of financial safety-net, along the supervisors of the market. Hence, an increased preoccupation for applied corporate governance has developed and Core Principles for Effective Deposit Insurance Systems have been adopted by the Basel Committee on Banking Supervision together with International Association of Deposit Insurers. Concurrently, the EU Commission has put forward a process for reviewing the Directives 94/39 and 09/14 EC, in order to make the European DGS more prepared to deal with above mentioned issues and bestow increased confidence upon depositors.

  19. Guaranteed performance in reaching mode of sliding mode controlled systems

    Indian Academy of Sciences (India)

    G K Singh; K E Holé

    2004-02-01

    Conventionally, the parameters of a sliding mode controller (SMC) are selected so as to reduce the time spent in the reaching mode. Although, an upper bound on the time to reach (reaching time) the sliding surface is easily derived, performance guarantee in the state/error space needs more consideration. This paper addresses the design of constant plus proportional rate reaching law-based SMC for second-order nonlinear systems. It is shown that this controller imposes a bounding second-order error-dynamics, and thus guarantees robust performance during the reaching phase. The choice of the controller parameters based on the time to reach a desirable level of output tracking error (OTE), rather than on the reaching time is proposed. Using the Lyapunov theory, it is shown that parameter selections, based on the reaching time criterion, may need substantially larger time to achieve the OTE. Simulation results are presented for a nonlinear spring-massdamper system. It is seen that parameter selections based on the proposed OTE criterion, result in substantially quicker tracking, while using similar levels of control effort.

  20. Robust guaranteed cost observer design for linear uncertain jump systems with state delays

    Institute of Scientific and Technical Information of China (English)

    FU Yan-ming; ZHANG Bo; DUAN Guang-ren

    2008-01-01

    This paper deals with the robust guaranteed cost observer with guaranteed cost performance for a class of linear uncertain jump systems with state delay. The transition of the jumping parameters in systems is governed by a finite-state Markov process. Based on the stability theory in stochastic differential equations, a sufficient condition on the existence of the proposed robust guaranteed cost observer is derived. Robust guaran-teed cost observers are designed in terms of a set of linear coupled matrix inequalities. A convex optimization problem with LMI constraints is formulated to design the suboptimal guaranteed cost observers.

  1. Methicillin-resistant Staphylococcus aureus: the superbug.

    Science.gov (United States)

    Ippolito, Giuseppe; Leone, Sebastiano; Lauria, Francesco N; Nicastri, Emanuele; Wenzel, Richard P

    2010-10-01

    Over the last decade, methicillin-resistant Staphylococcus aureus (MRSA) strains have emerged as serious pathogens in the nosocomial and community setting. Hospitalization costs associated with MRSA infections are substantially greater than those associated with methicillin-sensitive S. aureus (MSSA) infections, and MRSA has wider economic effects that involve indirect costs to the patient and to society. In addition, there is some evidence suggesting that MRSA infections increase morbidity and the risk of mortality. Glycopeptides are the backbone antibiotics for the treatment of MRSA infections. However, several recent reports have highlighted the limitations of vancomycin, and its role in the management of serious infections is now being reconsidered. Several new antimicrobials demonstrate in vitro activity against MRSA and other Gram-positive bacteria. Data from large surveys indicate that linezolid, daptomycin, and tigecycline are almost universally active against MRSA. This review will briefly discuss the epidemiology, costs, outcome, and therapeutic options for the management of MRSA infections. PMID:20851011

  2. Curcumin Reverse Methicillin Resistance in Staphylococcus aureus

    OpenAIRE

    Su-Hyun Mun; Sung-Bae Kim; Ryong Kong; Jang-Gi Choi; Youn-Chul Kim; Dong-Won Shin; Ok-Hwa Kang; Dong-Yeul Kwon

    2014-01-01

    Curcumin, a natural polyphenolic flavonoid extracted from the rhizome of Curcuma longa L., was shown to possess superior potency to resensitize methicillin-resistant Staphylococcus aureus (MRSA) to antibiotics. Previous studies have shown the synergistic activity of curcumin with β-lactam and quinolone antibiotics. Further, to understand the anti-MRSA mechanism of curcumin, we investigated the potentiated effect of curcumin by its interaction in diverse conditions. The mechanism of anti-MRSA ...

  3. Xanthgranulomatous pyelonephritis associated with Staphylococcus aureus.

    Science.gov (United States)

    Al-Hwiesh, Abdulla K

    2007-11-01

    A 44-year old man with xanthogranulomatous pyelonephritis presented with abdominal distention, left lumber pain, fever, loss of appetite, and loss of weight. He had been known to have diabetes mellitus type II for 20 years, and he was diagnosed to have a left renal stone three months prior to this presentation. The patient's urine and the left psous abscess grew staphylococcus aureus. PMID:17951953

  4. Xanthgranulomatous pyelonephritis associated with staphylococcus aureus

    International Nuclear Information System (INIS)

    A 44-year-old man with xanthgranulomatous pyelonephritis presented with abdominal distention, left lumber pain, fever, loss of appetite and loss of weight. He had been known to have diabetes mellitus type II for 20 years and he was diagnosed to have a left renal stone three months prior to this presentation. The patient's urine and the left psous abscess grew staphylococcus aureus. (author)

  5. Aspartate inhibits Staphylococcus aureus biofilm formation.

    Science.gov (United States)

    Yang, Hang; Wang, Mengyue; Yu, Junping; Wei, Hongping

    2015-04-01

    Biofilm formation renders Staphylococcus aureus highly resistant to conventional antibiotics and host defenses. Four D-amino acids (D-Leu, D-Met, D-Trp and D-Tyr) have been reported to be able to inhibit biofilm formation and disassemble established S. aureus biofilms. We report here for the first time that both D- and L-isoforms of aspartate (Asp) inhibited S. aureus biofilm formation on tissue culture plates. Similar biofilm inhibition effects were also observed against other staphylococcal strains, including S. saprophyticus, S. equorum, S. chromogenes and S. haemolyticus. It was found that Asp at high concentrations (>10 mM) inhibited the growth of planktonic N315 cells, but at subinhibitory concentrations decreased the cellular metabolic activity without influencing cell growth. The decreased cellular metabolic activity might be the reason for the production of less protein and DNA in the matrix of the biofilms formed in the presence of Asp. However, varied inhibition efficacies of Asp were observed for biofilms formed by clinical staphylococcal isolates. There might be mechanisms other than decreasing the metabolic activity, e.g. the biofilm phenotypes, affecting biofilm formation in the presence of Asp. PMID:25687923

  6. Whole-Genome Sequence of Staphylococcus aureus Strain LCT-SA112

    OpenAIRE

    Wang, Junfeng; Liu, Yanhong; Wan, Daiwei; Fang, Xiangqun; Li, Tianzhi; Guo, Yinghua; Chang, De; Su, Longxiang; Wang, Yajuan; Zhao, Jiao; Liu, Changting

    2012-01-01

    Staphylococcus aureus is a facultative anaerobic Gram-positive coccal bacterium. S. aureus is the most common species of Staphylococcus to cause staphylococcal infections, which are very common in clinical medicine. Here we report the genome sequence of S. aureus strain LCT-SA112, which was isolated from S. aureus subsp. aureus CGMCC 1.230.

  7. [Raw milk-associated Staphylococcus aureus intoxication in children].

    Science.gov (United States)

    Giezendanner, N; Meyer, B; Gort, M; Müller, P; Zweifel, C

    2009-07-01

    Four hours after the consumption of raw goat milk, three Swiss children came down with emesis and diarrhea in July 2008. First investigations showed that the milk originated from a goat suffering from clinical mastitis (Staphylococcus aureus). In the milk sample from the untreated left udder, Staphylococcus aureus counts reached 5.0 x 10(7) CFU ml(-1). By PCR, the gene for the staphylococcal enterotoxin D was found in isolated strains. The consumption of raw milk is rarely associated with Staphylococcus aureus intoxications. Due to the flora naturally present in raw milk, Staphylococcus aureus normally cannot multiply sufficiently. However, in the present case, high Staphylococcus aureus counts were already present in the milk due to the mastitis of the goat. This amount sufficed to cause a Staphylococcus aureus intoxication in the children. PMID:19565455

  8. Obligation Guarantees in the New Romanian Civil Code

    Directory of Open Access Journals (Sweden)

    Silvia Lucia Cristea

    2013-08-01

    Full Text Available This study starts from the analysis of the juridical regime of the mortgage that “migrates” from the sphere of the real rights towards the juridical regime of the receivables’ rights! The article contains an analysis of the legal regime of the mortgage according to changes made by the new Romanian Civil Code. This idea is supported by legislative arguments deriving from regulations of the pledge in the new Romanian Civil Code (sect. 2, of the mortgage (sect. 3, from the comparison with other similar juridical institutions (sect. 3. The conclusions explain how useful is to change the juridical regime of the real guarantees and the international dimension of trade. The study is one of the latest presentations doctrinal matter, with special insight into mortgage securities, legal institution whole new for the Romanian Civil Code. Despite the fact all the observations are theoretical, novelty approach makes reading not only addresses their academics, and practitioners: judges, lawyers, notaries.

  9. A methodology aimed to guarantee technology continuity in health structures.

    Science.gov (United States)

    Miniati, R; Dori, F; Iadanza, E; Scatizzi, L; Niccolini, F; Sarti, A

    2011-01-01

    In healthcare the importance of clinical continuity is essential for both patients life and health organization activity. Since technology continuity is having more and more importance for the service continuity, a correct management of medical devices must be guided by criteria that ensure its safe, appropriate and economical use through a well planned purchase, appropriate preventive and corrective maintenance Indeed, the aim of health technology managers is to optimize the integration of external interventions assistance and internal technical service to guarantee an efficient and cost-effective maintenance system. This paper proposes an innovative carefully thought methodology which is aimed to provide technological and procedural actions which offer support to decision makers in technology management regarding the implementation of continuity in medical services and response to technology failures and emergency events. PMID:22254534

  10. Stateless and Delivery Guaranteed Geometric Routing on Virtual Coordinate System

    CERN Document Server

    Liu, Ke

    2008-01-01

    Stateless geographic routing provides relatively good performance at a fixed overhead, which is typically much lower than conventional routing protocols such as AODV. However, the performance of geographic routing is impacted by physical voids, and localization errors. Accordingly, virtual coordinate systems (VCS) were proposed as an alternative approach that is resilient to localization errors and that naturally routes around physical voids. However, VCS also faces virtual anomalies, causing their performance to trail geographic routing. In existing VCS routing protocols, there is a lack of an effective stateless and delivery guaranteed complementary routing algorithm that can be used to traverse voids. Most proposed solutions use variants of flooding or blind searching when a void is encountered. In this paper, we propose a spanning-path virtual coordinate system which can be used as a complete routing algorithm or as the complementary algorithm to greedy forwarding that is invoked when voids are encountere...

  11. AUTOMATION OF PLC PROGRAMMING WHEN IMPLEMENTING ALGORITHMS OF GUARANTEEING CONTROL

    Directory of Open Access Journals (Sweden)

    M. V. Levinskyi

    2015-05-01

    Full Text Available During developing programs for programmable logic controllers (PLCs the concept of model-oriented design is increasingly used. In particular, usage of Simulink PLC Coder is giving the opportunity to get SCL program codefrom Simulink model which contains certain dynamic elements. Then, for example, this SCL code can be transformed to functional blocks of the Simatic S7-300 (VIPA 300 PLC. This significantly reduces the timerequired to develop code in the language of SCL and reduces requirements for specialists’ qualification when developing control systems. In this article we provide an example of PLC programming automation whenimplementing algorithms of guaranteeing control (AGC. For certain types of technological processes it is typical to contain monotonically increasing function of the effectiveness with fixed one-way restriction in regulations. Forexample, in the grinders, presses, extruders the load current of the drive is stabilized using the change of feed. Energy efficiency of these plants will increase with increasing of the set point (SP to the controller of the drive loadcurrent stabilization loop. However, an increase in SP increases the probability of triggering appropriate protection, for example, as a result of random changes in the properties of raw materials. Therefore, to avoid this accident, thepower of driving motors is often unreasonably overrated. And in this case they are used with currents equal to the half of rated.Systems of guaranteeing control (SGC are used to solve the contradiction between the need to improvethe efficiency and increasing probability of an accident.

  12. Antibody responses in patients with invasive Staphylococcus aureus infections

    OpenAIRE

    Jacobsson, G; Colque-Navarro, P.; Gustafsson, E.; Andersson, R.; Möllby, R

    2010-01-01

    Abstract Correlation between antibody response and clinical outcome in Staphylococcus aureus bacteremia has yielded conflicting results. Immunization schedules have failed in clinical trials. Is the humoral response toward S. aureus of protective nature? A prospective study was performed in patients with invasive S. aureus (ISA) infections during the period 2003?2005. The antibody levels were determined at the beginning and at the end of treatment and one month later (n?=?96, n?=?7...

  13. Vitamin D sufficiency and Staphylococcus aureus infection in children.

    Science.gov (United States)

    Wang, Jeffrey W; Hogan, Patrick G; Hunstad, David A; Fritz, Stephanie A

    2015-05-01

    Vitamin D promotes epithelial immunity by upregulating antimicrobial peptides, including LL-37, which have bactericidal activity against Staphylococcus aureus. We found that children with vitamin D deficiency or insufficiency [25-hydroxyvitamin D recurrent, rather than primary, S. aureus skin or soft tissue infection. Vitamin D sufficiency may be one of a myriad of host and environmental factors that can be directly impacted to reduce the frequency of S. aureus skin and soft tissue infection. PMID:25860535

  14. Mapping the Distribution of Invasive Staphylococcus aureus across Europe

    OpenAIRE

    Grundmann, Hajo; Aanensen, David M.; van den Wijngaard, Cees C.; Brian G Spratt; Harmsen, Dag; Friedrich, Alexander W.; ,

    2010-01-01

    Editors' Summary Background The bacterium Staphylococcus aureus lives on the skin and in the nose of about a third of healthy people. Although S. aureus usually coexists peacefully with its human carriers, it is also an important disease-causing organism or pathogen. If it enters the body through a cut or during a surgical procedure, S. aureus can cause minor infections such as pimples and boils or more serious, life-threatening infections such as blood poisoning and pneumonia. Minor S. aureu...

  15. Infection control of Staphylococcus aureus : spa typing to elucidate transmission

    OpenAIRE

    Mernelius, Sara

    2015-01-01

    Staphylococcus aureus is a commensal of the human flora, primarily colonizing the anterior nares and throat, but it may also cause infections ranging from mild skin and soft tissue infections to severe diseases such as endocarditis and septicemia. S. aureus is also a major nosocomial problem increasing with the worldwide dissemination of methicillin-resistant S. aureus (MRSA). The main vector for bacterial cross-transmission in healthcare settings is the hands of healthcare workers (HCWs). No...

  16. Staphylococcus aureus atsparumas antibiotikams ir fagotipų paplitimas

    OpenAIRE

    Kareivienė, Violeta; Pavilonis, Alvydas; Sinkutė, Gintarė; Liegiūtė, Sigutė; Gailienė, Greta

    2006-01-01

    Objective. The aim of this study was to identify the phage groups of Staphylococcus aureus strains, their prevalence, and resistance of different phage groups to antibiotics. Materials and methods. A total of 294 Staphylococcus aureus strains in Kaunas hospitals were obtained; they were phage typed and their resistance to antibiotics was determined. We used the method of routine dilution to test 17 antibiotics against the isolates. Susceptibility of Staphylococcus aureus to studied antibio...

  17. Threat of drug resistant Staphylococcus aureus to health in Nepal

    OpenAIRE

    Ansari, Shamshul; Nepal, Hari Prasad; Gautam, Rajendra; Rayamajhi, Nabin; Shrestha, Sony; Upadhyay, Goma; Acharya, Anju; Chapagain, Moti Lal

    2014-01-01

    Background Staphylococcus aureus is the most commonly isolated organism from the different clinical samples in hospital. The emergence and dissemination of methicillin resistant Staphylococcus aureus (MRSA) and growing resistance to non-beta-lactam antibiotics is making treatment of infections due to this organism increasingly difficult. Methods This study was conducted to determine the frequency of Staphylococcus aureus isolated from different clinical samples, rates of MRSA and full antibio...

  18. Epicutaneous Model of Community-Acquired Staphylococcus aureus Skin Infections

    OpenAIRE

    Prabhakara, Ranjani; Foreman, Oded; De Pascalis, Roberto; Lee, Gloria M.; Plaut, Roger D.; Kim, Stanley Y.; Stibitz, Scott; Elkins, Karen L.; Merkel, Tod J.

    2013-01-01

    Staphylococcus aureus is one of the most common etiological agents of community-acquired skin and soft tissue infection (SSTI). Although the majority of S. aureus community-acquired SSTIs are uncomplicated and self-clearing in nature, some percentage of these cases progress into life-threatening invasive infections. Current animal models of S. aureus SSTI suffer from two drawbacks: these models are a better representation of hospital-acquired SSTI than community-acquired SSTI, and they involv...

  19. Strategic design of cost savings guarantee in energy performance contracting under uncertainty

    International Nuclear Information System (INIS)

    Highlights: • A methodology is proposed to assist Energy Service Companies to maintain competitiveness in winning bids. • Uncertainties within the energy cost savings are modeled stochastically using the Monte-Carlo simulation. • A strategic energy savings guarantee design curve is derived, where all points return as appropriate guarantees. • A campus case is presented to demonstrate the applicability for finding appropriate guaranteed savings value. - Abstract: Among the key barriers to profit in Energy Performance Contracting (EPC) are uncertainties about attaining the realized energy cost savings and potential disputes over the guaranteed cost savings. In this paper, a methodology has been proposed to assist the Energy Service Company (ESCO): (1) to evaluate the risk threshold if the guarantee has already been made, and (2) to determine the guarantee design, if the guarantee has not been made yet, that not only promises the ESCO’s profitability from EPC but also maintains its competitiveness to win the bid. Uncertainties within the energy cost savings are modeled stochastically using Monte-Carlo simulation, taking both the energy price fluctuation and the facility performance variability into account. Based on that, a strategic energy savings guarantee design curve is derived, that all the points on it would return as appropriate guarantees. Finally, a campus case is presented to demonstrate the applicability for finding the appropriate guaranteed savings value. This method is also worth popularizing in similar performance-based projects

  20. VISA/VRSA (Vancomycin-Intermediate/Resistant Staphylococcus aureus) in Healthcare Settings

    Science.gov (United States)

    ... to vancomycin and other antimicrobial agents. What is Staphylococcus aureus? Staphylococcus aureus is a bacterium commonly found on the ... control personnel. Investigation and Control of Vancomycin-Resistant Staphylococcus aureus (VRSA) [PDF - 300 KB] - This document is ...

  1. Staphylococcus aureus: resistance pattern and risk factors

    Directory of Open Access Journals (Sweden)

    Mohammad Naghavi-Behzad

    2015-03-01

    Full Text Available Introduction: Methicillin resistant Staphylococcus aureus (MRSA has emerged as a nosocomial pathogen of major worldwide importance and is an increasingly frequent cause of community-acquired infections. In this study, different risk factors and MRSA resistance pattern were investigated. Methods: In a 24 months period, all of the patients who were confined to bed in the surgery ward were included in the study. Then they were assessed to find out as if they had MRSA infection when hospitalized and once when they were discharged. Almost 48 h after admission, when patients were discharged, social and medical histories were acquired. Acquired samples were examined. Results: During the present study of 475 patients, 108 patients (22.8% had S. aureus. About frequency of antibiotic resistance among collected S. aureus colonies, erythromycin resistance, was the most frequent antibiotic resistance, also resistance to vancomycin was 0.4% that was the least. Only hospitalization duration had statistically significant correlation with antibiotic resistance, also resistance to erythromycin had statistically significant relation with history of surgery and alcohol consumption. Of all 34 MRSA species, 22 (64.7% samples were resistant to erythromycin, 17 (50.0% resistant to cefoxitin, 5 (14.7% resistant to mupirocin, 1 (2.9% resistant to vancomycin and 1 (2.9% resistant to linezolid. Conclusion: The results of the current study show that among hospitalized patients, there is resistance against methicillin. Since based on results of the study there is resistance against oxacillin and erythromycin in most cases, administering appropriate antibiotics have an important role in minimizing the resistance burden among bacterial species.

  2. Guaranteed cost control of uncertain system with input time-delay

    Institute of Scientific and Technical Information of China (English)

    胡乃平; 唐功友

    2003-01-01

    For the uncertain continuous-time systems with input time-delay that widely exist in the production processes, we can get the existent conditions for the guaranteed cost control of these systems by using the Lyapunov stability theory, linear matrix inequalities theory and quadratic cost criterion. We can achieve the guaranteed cost control of this system by solving a matrix inequality. A state feed back guaranteed cost control law can be constructed by solving certain parameter-dependent Riccati matrix equation.

  3. Stochastic Finite-Time Guaranteed Cost Control of Markovian Jumping Singular Systems

    OpenAIRE

    Xiaowu Mu; Yingqi Zhang; Caixia Liu

    2011-01-01

    The problem of stochastic finite-time guaranteed cost control is investigated for Markovian jumping singular systems with uncertain transition probabilities, parametric uncertainties, and time-varying norm-bounded disturbance. Firstly, the definitions of stochastic singular finite-time stability, stochastic singular finite-time boundedness, and stochastic singular finite-time guaranteed cost control are presented. Then, sufficient conditions on stochastic singular finite-time guaranteed cost ...

  4. Do not pay for a Danish interest guarantee. The law of the triple blow

    DEFF Research Database (Denmark)

    Guillén, Montserrat; Konicz, Agnieszka Karolina; Perch Nielsen, Jens;

    2013-01-01

    We have investigated the performance of pension schemes of with-profit policies containing a guaranteed minimum rate of return and we have found that the price of the guarantee measured in terms of lost returns is enormous. We use simple simulations rather than complex pricing methods to illustrate...... that the price of an interest guarantee is high in pension products that are currently commercialised in the market. We have found that the customer loses up to about 0.75% yearly in the rate of return when an interest guarantee is purchased, compared to the return of an equivalent saving strategy with...

  5. A Scheduling Discipline for Latency and Bandwidth Guarantees in Asynchronous Network-on-Chip

    DEFF Research Database (Denmark)

    Bjerregaard, Tobias; Sparsø, Jens

    2005-01-01

    Guaranteed services (GS) are important in that they provide predictability in the complex dynamics of shared communication structures. This paper discusses the implementation of GS in asynchronous Network-on-Chip. We present a novel scheduling discipline called Asynchronous Latency Guarantee (ALG......) scheduling, which provides latency and bandwidth guarantees in accessing a shared media, e.g. a physical link shared between a number of virtual channels. ALG overcomes the drawbacks of existing scheduling disciplines, in particular the coupling between latency and bandwidth guarantees. A 0.12 &956;m CMOS...

  6. Staphylococcus aureus bacteriuria as a prognosticator for outcome of Staphylococcus aureus bacteremia: a case-control study

    Directory of Open Access Journals (Sweden)

    Weinstein Robert A

    2010-07-01

    Full Text Available Abstract Background When Staphylococcus aureus is isolated in urine, it is thought to usually represent hematogenous spread. Because such spread might have special clinical significance, we evaluated predictors and outcomes of S. aureus bacteriuria among patients with S. aureus bacteremia. Methods A case-control study was performed at John H. Stroger Jr. Hospital of Cook County among adult inpatients during January 2002-December 2006. Cases and controls had positive and negative urine cultures, respectively, for S. aureus, within 72 hours of positive blood culture for S. aureus. Controls were sampled randomly in a 1:4 ratio. Univariate and multivariable logistic regression analyses were done. Results Overall, 59% of patients were African-American, 12% died, 56% of infections had community-onset infections, and 58% were infected with methicillin-susceptible S. aureus (MSSA. Among 61 cases and 247 controls, predictors of S. aureus bacteriuria on multivariate analysis were urological surgery (OR = 3.4, p = 0.06 and genitourinary infection (OR = 9.2, p = 0.002. Among patients who died, there were significantly more patients with bacteriuria than among patients who survived (39% vs. 17%; p = 0.002. In multiple Cox regression analysis, death risks in bacteremic patients were bacteriuria (hazard ratio 2.9, CI 1.4-5.9, p = 0.004, bladder catheter use (2.0, 1.0-4.0, p = 0.06, and Charlson score (1.1, 1.1-1.3, p = 0.02. Neither length of stay nor methicillin-resistant Staphylococcus aureus (MRSA infection was a predictor of S. aureus bacteriuria or death. Conclusions Among patients with S. aureus bacteremia, those with S. aureus bacteriuria had 3-fold higher mortality than those without bacteriuria, even after adjustment for comorbidities. Bacteriuria may identify patients with more severe bacteremia, who are at risk of worse outcomes.

  7. Intracellular proliferation of S. aureus in osteoblasts and effects of rifampicin and gentamicin on S. aureus intracellular proliferation and survival

    Directory of Open Access Journals (Sweden)

    W Mohamed

    2014-10-01

    Full Text Available Staphylococcus aureus is the most clinically relevant pathogen regarding implant-associated bone infection and its capability to invade osteoblasts is well known. The aim of this study was to investigate firstly whether S. aureus is not only able to invade but also to proliferate within osteoblasts, secondly to delineate the mechanism of invasion and thirdly to clarify whether rifampicin or gentamicin can inhibit intracellular proliferation and survival of S. aureus. The SAOS-2 osteoblast-like cell line and human primary osteoblasts were infected with S. aureus EDCC5055 and S. aureus Rosenbach 1884. Both S. aureus strains were able to invade efficiently and to proliferate within human osteoblasts. Immunofluorescence microscopy showed intracellular invasion of S. aureus and transmission electron microscopy images could demonstrate bacterial division as a sign of intracellular proliferation as well as cytosolic bacterial persistence. Cytochalasin D, the major actin depolymerisation agent, was able to significantly reduce S. aureus invasion, suggesting that invasion was enabled by promoting actin rearrangement at the cell surface. 7.5 μg/mL of rifampicin was able to inhibit bacterial survival in SAOS-2 cells with almost complete elimination of bacteria after 4 h. Gentamicin could also kill intracellular S. aureus in a dose-dependent manner, an effect that was significantly lower than that observed using rifampicin. In conclusion, S. aureus is not only able to invade but also to proliferate in osteoblasts. Invasion seems to be associated with actin rearrangement at the cell surface. Rifampicin is effective in intracellular eradication of S. aureus whereas gentamicin only poorly eliminates intracellularly replicating bacteria.

  8. Relieving the Wireless Infrastructure: When Opportunistic Networks Meet Guaranteed Delays

    CERN Document Server

    Whitbeck, John; Leguay, Jérémie; Conan, Vania; de Amorim, Marcelo Dias

    2010-01-01

    Major wireless operators are nowadays facing network capacity issues in striving to meet the growing demands of mobile users. However, 3G-enabled devices increasingly benefit from ad hoc radio connectivity (e.g., Wi-Fi). In this context of hybrid connectivity, we propose Push-and-track, a content dissemination framework that harnesses ad hoc communication opportunities to minimize the load on the wireless infrastructure while guaranteeing tight delivery delays. It achieves this through a control loop that collects user-sent acknowledgements to determine if new copies need to be reinjected into the network through the 3G interface. Push-and-Track includes multiple strategies to determine how many copies of the content should be injected and to whom. The short delay-tolerance of common content, such as news or road traffic updates, make them suitable for such a system. Based on a realistic large-scale vehicular dataset from the city of Bologna composed of more than 10,000 vehicles, we demonstrate that Push-and-...

  9. Guaranteed Blind Sparse Spikes Deconvolution via Lifting and Convex Optimization

    Science.gov (United States)

    Chi, Yuejie

    2016-06-01

    Neural recordings, returns from radars and sonars, images in astronomy and single-molecule microscopy can be modeled as a linear superposition of a small number of scaled and delayed copies of a band-limited or diffraction-limited point spread function, which is either determined by the nature or designed by the users; in other words, we observe the convolution between a point spread function and a sparse spike signal with unknown amplitudes and delays. While it is of great interest to accurately resolve the spike signal from as few samples as possible, however, when the point spread function is not known a priori, this problem is terribly ill-posed. This paper proposes a convex optimization framework to simultaneously estimate the point spread function as well as the spike signal, by mildly constraining the point spread function to lie in a known low-dimensional subspace. By applying the lifting trick, we obtain an underdetermined linear system of an ensemble of signals with joint spectral sparsity, to which atomic norm minimization is applied. Under mild randomness assumptions of the low-dimensional subspace as well as a separation condition of the spike signal, we prove the proposed algorithm, dubbed as AtomicLift, is guaranteed to recover the spike signal up to a scaling factor as soon as the number of samples is large enough. The extension of AtomicLift to handle noisy measurements is also discussed. Numerical examples are provided to validate the effectiveness of the proposed approaches.

  10. Nuclear power: status, outlook, guarantees of sustainable development

    International Nuclear Information System (INIS)

    Full text: The principal advantages of nuclear power - almost unlimited fuel resources, its high energy capacity, ecological compatibility with a possibility of high wastes concentration - determine the large-scale nuclear power development. The signs of large-scale power - a large rate (dozens of percent) in electricity production, diverse areas (electricity, heat supply, technologies, transport) and media of application (land, ocean, space), extension of number of user countries, diversified power systems (centralized, autonomous), obligatory reproduction and reuse of produced fuel - create various requirements to nuclear power installations of the future. Economic efficiency and competitiveness, safety (of reactors and fuel cycle with waste), proper characteristics of nuclear fuel reproduction, guarantees of nuclear arm's non-proliferation and, particularly, public acceptance are the conditions of such nuclear power development. The up-to-date situation is the following: the 441 nuclear power-generation units with total installed power of 377.36 GW el. (in 31 countries) supply by 1/6 part of the world electric-power consumptions. The 32 units are in stage of the construction. To the present mid-century the level of the nuclear power production, as supposed, must be increased 4-5 times at the following scenario of a regional distribution of nuclear electric powers, GW: USA, Europe and developed countries of Eastern Asia - 1000, FSU-countries - 100 and developing countries - 400

  11. On routing algorithms with end-to-end delay guarantees

    Energy Technology Data Exchange (ETDEWEB)

    Rao, N.S.V.; Batsell, S.G.

    1998-11-01

    The authors consider the transmission of a message of size r from a source to a destination with guarantees on the end-to-end delay over a computer network with n nodes and m links. There are three sources of delays: (a) propagation delays along the links, (b) delays due to bandwidth availability on the links, and (c) queuing delays at the intermediate nodes. First, the authors consider that delays on various links and nodes are given as functions of the message size. If the delay in (b) is a non-increasing function of the bandwidth, they propose O(m{sup 2} + mn log n) time algorithm to compute a path with the minimum end-to-end delay for any given message size r. They then consider that the queuing delay in (c) is a random variable correlated with the message size according to an unknown distribution. At each node, the measurements of queuing delays and message sizes are available. They propose two algorithms to compute paths whose delays are close to optimal delays with a high probability, irrespective of the distribution of the delays, and based entirely on the measurements of sufficient size.

  12. Psychology of nuclear safeguards. [Cannot guarantee preventing diversion, only deter

    Energy Technology Data Exchange (ETDEWEB)

    Parker, L.

    1978-08-17

    There is an essential confusion in the way we look at safeguards. They cannot guarantee to prevent diversions: at best, they can deter. While successful acts of deterrence pass unnoticed, failures will be widely publicized. Therefore, we will judge safeguards systems not on a fair balance between their successes and failures, but exclusively on what we believe to be their failures. Effectively, we will be demanding an impossibly high standard of performance, and safeguards authorities will be tempted to conceal diversions rather than disappoint our expectations. Perhaps it is appropriate that safeguards are primarily psychological instruments: Parker argues that their essence is credibility, and that this does not necessarily depend on the hard reality of their performance. Brian Johnson claimed (New Scientist, vol 74, p 189) that safeguards have engendered a sense of security by linking the commitment of many states not to develop nuclear weapons, and that the Non-Proliferation Treaty has helped condition domestic political attitudes against starting up nuclear weapons programs. Perhaps the common view that safeguards are legal instruments has served the psychological purpose of supplying a language in which states with widely differing interests can explore the fundamental incompatibility between non-proliferation and the search for convenient energy sources. If this is true then it cannot be said that the NPT is a failure, merely that its success does not correspond to its apparent purposes.

  13. Functional modules of sigma factor regulons guarantee adaptability and evolvability

    Science.gov (United States)

    Binder, Sebastian C.; Eckweiler, Denitsa; Schulz, Sebastian; Bielecka, Agata; Nicolai, Tanja; Franke, Raimo; Häussler, Susanne; Meyer-Hermann, Michael

    2016-02-01

    The focus of modern molecular biology turns from assigning functions to individual genes towards understanding the expression and regulation of complex sets of molecules. Here, we provide evidence that alternative sigma factor regulons in the pathogen Pseudomonas aeruginosa largely represent insulated functional modules which provide a critical level of biological organization involved in general adaptation and survival processes. Analysis of the operational state of the sigma factor network revealed that transcription factors functionally couple the sigma factor regulons and significantly modulate the transcription levels in the face of challenging environments. The threshold quality of newly evolved transcription factors was reached faster and more robustly in in silico testing when the structural organization of sigma factor networks was taken into account. These results indicate that the modular structures of alternative sigma factor regulons provide P. aeruginosa with a robust framework to function adequately in its environment and at the same time facilitate evolutionary change. Our data support the view that widespread modularity guarantees robustness of biological networks and is a key driver of evolvability.

  14. Can Fixation be Guaranteed in the Generalized Moran Process?

    CERN Document Server

    Diaz, Josep; Mertzios, George B; Richerby, David; Serna, Maria; Spirakis, Paul G

    2012-01-01

    The Moran process models the spread of genetic mutations through a population. A mutant with relative fitness r is introduced into a population and the system evolves, either reaching fixation (in which every individual is a mutant) or extinction (in which none is). In a widely cited paper (Nature, 2005), Lieberman, Hauert and Nowak generalize the model to populations on the vertices of graphs. They claim to have discovered a class of graphs (called "superstars"), with a parameter k, within which the probability of fixation tends to 1-r^{-k} as graphs get larger. Thus, they say that these graphs "guarantee the fixation of any advantageous mutant". They give a non-rigorous proof of the claimed limiting fixation probability, which we show to be incorrect. Specifically, for k=5, we show that the true fixation probability is at most 1-1/j(r) where j(r)=Theta(r^4), contrary to the claimed result. Since the claimed proof is flawed, and no others are known, we investigate the claim via simulation. Lieberman et al. v...

  15. Ensuring QOS Guarantees in a Hybrid OCS/OBS Network

    CERN Document Server

    S, Sunish Kumar O

    2012-01-01

    The bursting aggregation assembly in edge nodes is one of the key technologies in OBS (Optical Burst Switching) network, which has a direct impact on flow characteristics and packet loss rate. An optical burst assembly technique supporting QoS is presented through this paper, which can automatically adjust the threshold along with the increasing and decreasing volume of business, reduce the operational burst, and generate corresponding BDP (Burst Data Packet) and BCP (Burst Control Packet). In addition to the burst aggregation technique a packet recovery technique by restoration method is also described. The data packet loss due to the physical optical link failure is not currently included in the QoS descriptions. This link failure is also a severe problem which reduces the data throughput of the transmitter node. A mechanism for data recovery from this link failure is vital for guaranteeing the QoS demanded by each user. So this paper will also discusses a specific protocol for reducing the packet loss by u...

  16. Duplex Identification of Staphylococcus aureus by Aptamer and Gold Nanoparticles.

    Science.gov (United States)

    Chang, Tianjun; Wang, Libo; Zhao, Kexu; Ge, Yu; He, Meng; Li, Gang

    2016-06-01

    Staphylococcus aureus is the top common pathogen causing infections and food poisoning. Identification of S. aureus is crucial for the disease diagnosis and regulation of food hygiene. Herein, we report an aptamer-AuNPs based method for duplex identification of S. aureus. Using AuNPs as an indicator, SA23, an aptamer against S. aureus, can well identify its target from Escherichia coli, Listeria monocytogenes and Pseudomonas aeruginosa. Furthermore, we find citrate-coated AuNPs can strongly bind to S. aureus, but not bind to Salmonella enterica and Proteus mirabilis, which leads to different color changes in salt solution. This colorimetric response is capable of distinguishing S. aureus from S. enteritidis and P. mirabilis. Thus, using the aptasensor and AuNPs together, S. aureus can be accurately identified from the common pathogens. This duplex identification system is a promising platform for simple visual identification of S. aureus. Additionally, in the aptasensing process, bacteria are incubated with aptamers and then be removed before the aptamers adding to AuNPs, which may avoid the interactions between bacteria and AuNPs. This strategy can be potentially applied in principle to detect other cells by AuNPs-based aptasensors. PMID:27427591

  17. Lysostaphin in treatment of neonatal Staphylococcus aureus infection.

    Science.gov (United States)

    Oluola, Okunola; Kong, Lingkun; Fein, Mindy; Weisman, Leonard E

    2007-06-01

    This study describes lysostaphin's effect against methicillin-sensitive Staphylococcus aureus in suckling rats. Standard techniques determined minimal inhibitory and bactericidal concentrations, pharmacokinetics, and efficacy. The numbers of surviving rats after vancomycin, oxacillin, and lysostaphin treatment were comparable and were different from that of controls (P < 0.00001). Lysostaphin appears effective in the treatment of neonatal S. aureus infection. PMID:17420212

  18. Susceptibility of methicillin-resistant Staphylococcus aureus to lysostaphin.

    OpenAIRE

    Huber, M M; Huber, T. W.

    1989-01-01

    One hundred and eleven isolates of methicillin-resistant Staphylococcus aureus recovered from patients at the Olin E. Teague Veterans Center from March 1983 to April 1987 were as susceptible to lysis by lysostaphin as methicillin-susceptible S. aureus controls were.

  19. Mechanism of resistance to some cephalosporins in Staphylococcus aureus.

    OpenAIRE

    Kono, M; Sasatsu, M; O'Hara, K; Shiomi, Y.; HAYASAKA, T.

    1983-01-01

    The mechanism of resistance to some cephalosporins in Staphylococcus aureus strains was investigated with high-pressure liquid chromatography and nuclear magnetic resonance spectrometry. Drug inactivation by penicillinase was found to be the main mechanism of resistance to cefazolin, cephaloridine, and cephalothin in S. aureus.

  20. Activity of and Resistance to Moxifloxacin in Staphylococcus aureus

    OpenAIRE

    Ince, Dilek; Zhang, Xiamei; Hooper, David C.

    2003-01-01

    Moxifloxacin has enhanced potency against Staphylococcus aureus, lower propensity to select for resistant mutants, and higher bactericidal activity against highly resistant strains than ciprofloxacin. Despite similar activity against purified S. aureus topoisomerase IV and DNA gyrase, it selects for topoisomerase IV mutants, making topoisomerase IV the preferred target in vivo.

  1. Changing Trends in Resistance Pattern of Methicillin Resistant Staphylococcus aureus

    OpenAIRE

    Kali, Arunava; Stephen, Selvaraj; Umadevi, Sivaraman; Kumar, Shailesh; Joseph, Noyal Mariya; Srirangaraj, Sreenivasan

    2013-01-01

    Background: Methicillin resistance in Staphylococcus aureus is associated with multidrug resistance, an aggressive course, increased mortality and morbidity in both community and health care facilities. Monitoring of newly emerging and prevalent Methicillin Resistant Staphylococcus aureus (MRSA) strains for their resistance patterns to conventional as well as novel drugs, are essential for infection control.

  2. Effect of Mupirocin on Nasal Carriage of Staphylococcus Aureus

    NARCIS (Netherlands)

    M. Bulanda; M. Gruszka; B. Heczko

    1989-01-01

    textabstractMupirocin eliminates nasal carriage of staphylococcal aureus among medical and surgical personnel for periode varying from several weeks upto one year. In persons recolonized after therapy densites of S. aureus population in nares were much lower than in the same persons before therapy.

  3. A porcine model of haematogenous brain infectionwith staphylococcus aureus

    DEFF Research Database (Denmark)

    Astrup, Lærke Boye; Agerholm, Jørgen Steen; Nielsen, Ole Lerberg;

    2012-01-01

    A PORCINE MODEL OF HAEMATOGENOUS BRAIN INFECTION WITH STAPHYLOCOCCUS AUREUS Astrup Lærke1, Agerholm Jørgen1, Nielsen Ole1, Jensen Henrik1, Leifsson Páll1, Iburg Tine2. 1: Faculty of Health and Medical Sciences, University of Copenhagen, Denmark boye@life.ku.dk 2: National Veterinary Institute......, Uppsala, Sweden Introduction Staphylococcus aureus (S.aureus) is a common cause of sepsis and brain abscesses in man and a frequent cause of porcine pyaemia. Here we present a porcine model of haematogenous S. aureus-induced brain infection. Materials and Methods Four pigs had two intravenous catheters...... inserted surgically, one in a. carotis communis and one in v. jugularis externa. All pigs received 106 CFU/kg body weight S. aureus through the arterial catheter. Bacteria were either suspended in isotonic saline infused at constant flow for 60 minutes (two pigs) or given as a bolus injection of autologoue...

  4. Antimicrobial drug resistance ofStaphylococcus aureus in dairy products

    Institute of Scientific and Technical Information of China (English)

    Sasidharan S; Prema B; Yoga Latha L

    2011-01-01

    Objective:To evaluate the prevalence of multidrug resistantStaphylococcus aureus(S. aureus) in dairy products.Methods:Isolation and identification ofS. aureus were performed in3 dairy-based food products. The isolates were tested for their susceptibility to5 different common antimicrobial drugs.Results:Of50 samples examined,5 (10%) were contaminated with S. aureus. Subsequently, the5 isolates were subjected to antimicrobial resistance pattern using five antibiotic discs (methicillin, vancomycin, kanamycin, chloramphenicol and tetracycline). Sample 29 showed resistance to methicillin and vancomycin. Sample18 showed intermediate response to tetracycline. The other samples were susceptible to all the antibiotics tested.Conclusions:The results provide preliminary data on sources of food contamination which may act as vehicles for the transmission of antimicrobial-resistantStaphylococcus.Therefore, it enables us to develop preventive strategies to avoid the emergence of new strains of resistantS. aureus.

  5. Characterization of methicillin-resistant Staphylococcus aureus Sequence Type 398

    DEFF Research Database (Denmark)

    Christiansen, Mette Theilgaard

    Staphylococcus aureus is an opportunistic pathogen that colonizes the nares and skin surfaces of several animal species, including man. S. aureus can cause a wide variety of infections ranging from superficial soft tissue and skin infections to severe and deadly systemic infections. Traditionally S...... for LA-MRSA ST398 survival on porcine skin and nasal epithelium ex vivo were identified. These genes could represent targets for de-colonization, which could help prevent further spread and adaption of LA-MRSA ST398. Manuscript III describes the construction of the S. aureus VirulenceFinder database....... The database can be applied for identification of virulence genes in S. aureus using whole genome 5 sequence data. The S. aureus VirulenceFinder will be part of the tool package generated for the Centre for Genomic Epidemiology (CGE) (www.genomicepidemiology.org)....

  6. Pricing of the Cross-Currency Interest Rate Guarantee Embedded in Financial Contracts in a LIBOR Market Model

    OpenAIRE

    Tsung-Yu Hsieh; Chi-Hsun Chou; Son-Nan Chen

    2015-01-01

    We derive the pricing formula for the financial contracts, such as guaranteed investment contracts (GICs), life insurance contracts, pension plans, and others, with the guaranteed minimum rate of return set relative to a LIBOR interest rate. Further, we analyze the guaranteed contracts in which the asset that provides the underlying return for the contract and the guaranteed interest rate are denominated in different currencies, which is a common practice. The guaranteed contracts with the ab...

  7. Where does a Staphylococcus aureus vaccine stand?

    Science.gov (United States)

    Fowler, V G; Proctor, R A

    2014-05-01

    In this review, we examine the current status of Staphylococcus aureus vaccine development and the prospects for future vaccines. Examination of the clinical trials to date show that murine models have not predicted success in humans for active or passive immunization. A key factor in the failure to develop a vaccine to prevent S. aureus infections comes from our relatively limited knowledge of human protective immunity. More recent reports on the elements of the human immune response to staphylococci are analysed. In addition, there is some controversy concerning the role of antibodies for protecting humans, and these data are reviewed. From a review of the current state of understanding of staphylococcal immunity, a working model is proposed. Some new work has provided some initial candidate biomarker(s) to predict outcomes of invasive infections and to predict the efficacy of antibiotic therapy in humans. We conclude by looking to the future through the perspective of lessons gleaned from the clinical vaccine trials. PMID:24476315

  8. Efektivitas Ekstrak Daun Jambu Biji Buah Putih Terhadap Pertumbuhan Staphylococcus aureus Dari Abses Dan Staphylococcus aureus (ATCC® 29213™)

    OpenAIRE

    Sinurat, Jojor

    2016-01-01

    Daun jambu biji mengandung senyawa aktif seperti tanin, triterpenoid, flavonoid, saponin yang mempunyai efek antibakteri. Mekanisme tanin sebagai antibakteri dengan mengkerutkan dinding sel dan membran sel, inaktivasi enzim, inaktivasi fungsi materi genetik bakteri. Flavonoid merusak sel bakteri, denaturasi protein, inaktivasi enzim dan menyebabkan lisis. Triterpenoid dan saponin menghambat pertumbuhan Staphylococcus aureus dengan cara merusak struktur membran sel. Staphylococcus aureus adala...

  9. μ distortions or running: A guaranteed discovery from CMB spectrometry

    Science.gov (United States)

    Cabass, Giovanni; Melchiorri, Alessandro; Pajer, Enrico

    2016-04-01

    We discuss the implications of a PIXIE-like experiment, which would measure μ -type spectral distortions of the cosmic microwave background (CMB) at a level of σμ=(1 /n )×10-8 , with n ≥1 representing an improved sensitivity (e.g. n =10 corresponds to PRISM). Using Planck data and considering the six-parameter Λ CDM model, we compute the posterior for μ8≡μ ×108 and find μ8=1.5 7-0.13+0.11 (68% C.L.). This becomes μ8=1.2 8-0.52+0.30 (68% C.L.) when the running αs of the spectral index is included. We point out that a sensitivity of about 3 ×PIXIE implies a guaranteed discovery: μ distortion is detected or αs≥0 is excluded (both at 95% C.L. or higher). This threshold sensitivity sets a clear benchmark for CMB spectrometry. For a combined analysis of PIXIE and current Planck data, we discuss the improvement on measurements of the tilt ns and the running αs and the dependence on the choice of the pivot. A fiducial running of αs=-0.01 (close to the Planck best fit) leads to a detection of negative running at 2 σ for 5 ×PIXIE . A fiducial running of αs=-0.02 , still compatible with Planck, requires 3 ×PIXIE to rule out αs=0 (at 95% C.L.). We propose a convenient and compact visualization of the improving constraints on the tilt, running and tensor-to-scalar ratio.

  10. Some Modeling Considerations in Deciding Multi-Year Guaranteed Tuition Rates.

    Science.gov (United States)

    Troutt, Marvin D.; And Others

    1995-01-01

    Discussion of guaranteed college tuition programs first explores their origins and reasons for continuing high inflation rates for tuition. The relationship between tuition increase and enrollment is examined, and many current guaranteed tuition plans are reviewed. Basic considerations in developing a model for a multiyear plan are outlined and a…

  11. 7 CFR 1493.50 - Certification requirements for obtaining payment guarantee.

    Science.gov (United States)

    2010-01-01

    ...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export... States law; (c) If the agricultural commodity is vegetable oil or a vegetable oil product, that none...

  12. 24 CFR 1000.426 - How does HUD review a guarantee application?

    Science.gov (United States)

    2010-04-01

    ... application? 1000.426 Section 1000.426 Housing and Urban Development Regulations Relating to Housing and Urban... URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES Federal Guarantees for Financing of Tribal Housing Activities § 1000.426 How does HUD review a guarantee application? The procedure for review of a...

  13. 20 CFR 229.10 - What the social security overall minimum guarantee is.

    Science.gov (United States)

    2010-04-01

    ... payable before the employee attains retirement age. An overall minimum may also be payable before age 62... guarantee is. 229.10 Section 229.10 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD RETIREMENT ACT SOCIAL SECURITY OVERALL MINIMUM GUARANTEE Social Security Overall Minimum...

  14. 13 CFR 108.820 - Financings in the form of guarantees.

    Science.gov (United States)

    2010-01-01

    ... VENTURE CAPITAL (âNMVCâ) PROGRAM Financing of Small Businesses by NMVC Companies Structuring Nmvc Company's Financing of Eligible Small Businesses § 108.820 Financings in the form of guarantees. (a) General... amount of the debt guaranteed, whichever is less....

  15. 34 CFR 682.513 - Factors affecting coverage of a loan under the loan guarantee.

    Science.gov (United States)

    2010-07-01

    ... 34 Education 3 2010-07-01 2010-07-01 false Factors affecting coverage of a loan under the loan guarantee. 682.513 Section 682.513 Education Regulations of the Offices of the Department of Education...) PROGRAM Federal Guaranteed Student Loan Programs § 682.513 Factors affecting coverage of a loan under...

  16. 78 FR 64175 - Hashemite Kingdom of Jordan Loan Guarantees Issued Under the Further Continuing Appropriations...

    Science.gov (United States)

    2013-10-28

    ... agreements. 233.14 Arbitration. 233.15 Notice. 233.16 Governing Law. Appendix A to Part 233--Application for... Payment and Further Guaranteed Payments, in each case from the date of default with respect to such... mail at the address set forth in the Note Register. ] Sec. 233.16 Governing Law. This Guarantee...

  17. Social Guarantees as a Stabilisation Institution under Conditions of Global Uncertainty

    Directory of Open Access Journals (Sweden)

    Salenkov Anton V.

    2013-12-01

    Full Text Available The article reveals a special role of social guarantees as an economic institution in overcoming uncertainty of impact of global transformational shifts on functioning and development of economic systems. It shows that, under conditions of global uncertainty, the institution of social guarantees plays the role of the border of permissible and acceptable changes in the national economy. It provides a comparative institutional characteristics of three social mechanisms of access to public resources for a person who has no possibility to ensure own vital activity by means of own labour and own property: social guarantees, loan, charity. It proves that social guarantees are a significant factor of optimisation of risk individual economic behaviour and take part in ensuring optimal level of trust in economy. It justifies the conclusion that social guarantees facilitate optimisation of correlation between preservation of social wealth and its development through participation in social selection of technological and institutional innovations.

  18. The molecular changing mechanism of Ampicillin-Sulbactam resistant Staphylococcus aureus towards Methicillin resistant Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Mieke Hemiawati Satari

    2005-12-01

    Full Text Available The aim of this study was to determine the molecular changing of S.aureus, which is resistant to Ampicillin-Sulbactam and then become resistant to Methicillin as a result of improper dosage. The study was conducted by isolating Ampicillin-Sulbactam resistant and Methicillin Resistant S.aureus (MRSA, afterwards an amplification process was performed by PCR (Polymerase Chain Reaction. to isolate the betalactamase enzyme regulator and PBP 2a genes. The result of this research showed that there were a deletion of few amino acids from the regulator gene, and a suspicion that the DNA sequence had been substituted from PBP 2 gene into PBP 2a (gen mec. This process had formed MRSA.

  19. Indole and 7-benzyloxyindole attenuate the virulence of Staphylococcus aureus.

    Science.gov (United States)

    Lee, Jin-Hyung; Cho, Hyun Seob; Kim, Younghoon; Kim, Jung-Ae; Banskota, Suhrid; Cho, Moo Hwan; Lee, Jintae

    2013-05-01

    Human pathogens can readily develop drug resistance due to the long-term use of antibiotics that mostly inhibit bacterial growth. Unlike antibiotics, antivirulence compounds diminish bacterial virulence without affecting cell viability and thus, may not lead to drug resistance. Staphylococcus aureus is a major agent of nosocomial infections and produces diverse virulence factors, such as the yellow carotenoid staphyloxanthin, which promotes resistance to reactive oxygen species (ROS) and the host immune system. To identify novel antivirulence compounds, bacterial signal indole present in animal gut and diverse indole derivatives were investigated with respect to reducing staphyloxanthin production and the hemolytic activity of S. aureus. Treatment with indole or its derivative 7-benzyloxyindole (7BOI) caused S. aureus to become colorless and inhibited its hemolytic ability without affecting bacterial growth. As a result, S. aureus was more easily killed by hydrogen peroxide (H₂O₂) and by human whole blood in the presence of indole or 7BOI. In addition, 7BOI attenuated S. aureus virulence in an in vivo model of nematode Caenorhabditis elegans, which is readily infected and killed by S. aureus. Transcriptional analyses showed that both indole and 7BOI repressed the expressions of several virulence genes such as α-hemolysin gene hla, enterotoxin seb, and the protease genes splA and sspA and modulated the expressions of the important regulatory genes agrA and sarA. These findings show that indole derivatives are potential candidates for use in antivirulence strategies against persistent S. aureus infection. PMID:23318836

  20. The changing epidemiology of Staphylococcus aureus bloodstream infection

    DEFF Research Database (Denmark)

    Galbraith, J.C.; Valiquette, G.; Kennedy, K.J.;

    2013-01-01

    Clin Microbiol Infect ABSTRACT: Although the epidemiology of Staphylococcus aureus bloodstream infection (BSI) has been changing, international comparisons are lacking. We sought to determine the incidence of S. aureus BSI and assess trends over time and by region. Population-based surveillance...... episodes of S. aureus BSI were identified. The overall annual incidence rate for S. aureus BSI was 26.1 per 100 000 population, and those for methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) were 24.2 and 1.9 per 100 000, respectively. Although the overall incidence...... of community-onset MSSA BSI (15.0 per 100 000) was relatively similar across regions, the incidence rates of hospital-onset MSSA (9.2 per 100 000), community-onset MRSA (1.0 per 100 000) and hospital-onset MRSA (0.8 per 100 000) BSI varied substantially. Whereas the overall incidence of S. aureus BSI did...

  1. Staphylococcus aureus: portadores entre manipuladores de alimentos Staphylococcus aureus: food handler carriers

    Directory of Open Access Journals (Sweden)

    Maria Stella Gonçalves Raddi

    1988-02-01

    Full Text Available Foram colhidas amostras de mãos e fossas nasais de 48 manipuladores de alimentos das principais casas comerciais da cidade de Araraquara, Estado de São Paulo (Brasil, e de 20 estudantes universitários. Dentre os indivíduos foram encontrados 44,1% e 34,8% que portavam Staphylococcus aureus em fossas nasais e mãos, respectivamente. Observou-se predomínio de fagotipos dos grupos I e III. Dos 12 portadores do microrganismo, concomitantemente em mãos e fossas nasais, 75,0% apresentaram cepas com vínculo epidemiológico. Os achados mostram o risco potencial representado pelas mãos nas intoxicações alimentares.Material was collected from the hands and nasal passages of forty-eight food handlers and twenty college students of Araraquara (S. Paulo State, Brazil and analized in order to evaluate the carrier function with regard to Staphylococcus aureus. The organism discovered in both samples of nine out of the twelve volunteers were of the same S. aureus phage types. The incidence of carriage on the hands was much greater in the handlers' group. These findings demonstrate the potential risk represented by hands in the transmission of food poisoning.

  2. Methicillin-resistant Staphylococcus aureus transmission

    DEFF Research Database (Denmark)

    Andersen, Leif Percival; Nielsen, Xiaohui

    2015-01-01

    increase the risk of contaminating hands, arms and the front of the uniform. Hand hygiene is therefore essential, but the use of protection gowns with long sleeves is also important in order to prevent transmission of MRSA. After culture of MRSA and implementation of specific precautions to prevent......INTRODUCTION: Even though methicillin-resistant Staphylococcus aureus (MRSA) is a common cause of nosocomial infections, it may often be difficult to evaluate the exact route of transmission. METHODS: In this study, we describe four cases of nosocomial transmission of MRSA in a hospital with a low...... transmission of MRSA, no further transmissions were observed. FUNDING: not relevant. TRIAL REGISTRATION: The data in this study are included in the routine surveillance of MRSA at Rigshospitalet and do not form part of a trial....

  3. Bovine Staphylococcus aureus: Subtyping, evolution, and zoonotic transfer.

    Science.gov (United States)

    Boss, R; Cosandey, A; Luini, M; Artursson, K; Bardiau, M; Breitenwieser, F; Hehenberger, E; Lam, Th; Mansfeld, M; Michel, A; Mösslacher, G; Naskova, J; Nelson, S; Podpečan, O; Raemy, A; Ryan, E; Salat, O; Zangerl, P; Steiner, A; Graber, H U

    2016-01-01

    Staphylococcus aureus is globally one of the most important pathogens causing contagious mastitis in cattle. Previous studies using ribosomal spacer (RS)-PCR, however, demonstrated in Swiss cows that Staph. aureus isolated from bovine intramammary infections are genetically heterogeneous, with Staph. aureus genotype B (GTB) and GTC being the most prominent genotypes. Furthermore, Staph. aureus GTB was found to be contagious, whereas Staph. aureus GTC and all the remaining genotypes were involved in individual cow disease. In addition to RS-PCR, other methods for subtyping Staph. aureus are known, including spa typing and multilocus sequence typing (MLST). They are based on sequencing the spa and various housekeeping genes, respectively. The aim of the present study was to compare the 3 analytic methods using 456 strains of Staph. aureus isolated from milk of bovine intramammary infections and bulk tanks obtained from 12 European countries. Furthermore, the phylogeny of animal Staph. aureus was inferred and the zoonotic transfer of Staph. aureus between cattle and humans was studied. The analyzed strains could be grouped into 6 genotypic clusters, with CLB, CLC, and CLR being the most prominent ones. Comparing the 3 subtyping methods, RS-PCR showed the highest resolution, followed by spa typing and MLST. We found associations among the methods but in many cases they were unsatisfactory except for CLB and CLC. Cluster CLB was positive for clonal complex (CC)8 in 99% of the cases and typically positive for t2953; it is the cattle-adapted form of CC8. Cluster CLC was always positive for tbl 2645 and typically positive for CC705. For CLR and the remaining subtypes, links among the 3 methods were generally poor. Bovine Staph. aureus is highly clonal and a few clones predominate. Animal Staph. aureus always evolve from human strains, such that every human strain may be the ancestor of a novel animal-adapted strain. The zoonotic transfer of IMI- and milk-associated strains

  4. Identification of the ClpX Regulon in Staphylococcus aureus

    DEFF Research Database (Denmark)

    Jelsbak, Lotte; Thomsen, Line Elnif; Ingmer, Hanne;

    Staphyloccous aureus is a major human pathogen capable of causing a wide spectrum of infections ranging from superficial wound infections to life-threatening endocarditis and toxic shock syndrome. Essential for S. aureus virulence is a large number of cell-surface-associated proteins and secreted...... we show here that almost 400 genes (15%) are influenced by the clpX deletion. Furthermore, ClpX not only regulates many virulence factors, but rather serves as a global regulator of central functions for S. aureus lifestyle and pathogenicity....

  5. The Present Situation of Chattel Guarantee System and Its Improvements - Taking the Angl of American Uniform Commercial Code (UCC)

    Institute of Scientific and Technical Information of China (English)

    仇晓光; 刘聪

    2011-01-01

    Although there are appropriate stipulations for the chattel guarantees in the law of China,this guarantee system could not adapt to the request of economy's development because of the following reasons:the scope of chattel guarantees is narrow according to relevant stipulations; the transaction cost of the chattel is high; mortgage registration of chattel is in a mess; lack of efficiently executive guarantee system,etc.To counter the problems existing in the present Chinese chattel guarantee system,in this article,the author proposed some corresponding suggestions and countermeasures,hoping to perfect the chattel guarantee system in China.

  6. The Impact of Guarantees on the Performance of Pension Saving Schemes: Insights from the Literature

    Directory of Open Access Journals (Sweden)

    Alexander Bohnert

    2015-11-01

    Full Text Available Guarantees are often seen as the key characteristics of pension saving products, but securing them can become costly and is of central relevance especially in the course of the current low interest rate environment. In this article, we deal with the question of how costly the typical types of guarantees are, in the sense that they reduce a pension saving scheme’s financial performance over time. In this context, we aim to provide a presentation of insights from selected literature studying the impact of point-to-point guarantees and cliquet-style interest rate guarantees on the performance of pension contracts. The comparative analysis emphasizes that, in most cases, guarantee costs are not negligible with regard to a contract’s financial performance, especially compared to benchmarks, and that customers knowingly opt for such guarantees (or not is, thus, indispensable. To further investigate the willingness-to-pay for guarantees in life insurance is an area for future research, in particular for innovative contract design.

  7. Separation of Staphylococcus aureus causing serious infections by electrophoretic techniques

    Czech Academy of Sciences Publication Activity Database

    Tesařová, Marie; Horká, Marie; Moravcová, Dana; Šťavíková, Lenka; Růžička, F.

    2014. s. 237-238. [Chemtech /14./. 22.10.2014-25.10.2014, Istambul] R&D Projects: GA MV VG20112015021 Institutional support: RVO:68081715 Keywords : Staphylococcus aureus * electrophoretic techniques Subject RIV: CB - Analytical Chemistry, Separation

  8. Improving Diagnosis and Treatment of Staphylococcus aureus Infections : Experimental Studies

    NARCIS (Netherlands)

    S. van den Berg (Sanne)

    2015-01-01

    markdownabstract__Abstract__ Staphylococcus aureus is an opportunistic pathogen that causes a variety of infections, ranging from mild skin infections like furuncles and impetigo, to severe, lifethreatening infections including endocarditis, osteomyelitis and pneumonia. Invasive infections are freq

  9. Delay-independent robust guaranteed-cost control for uncertain linear neutral systems

    Institute of Scientific and Technical Information of China (English)

    Li Hongfei; Zhou Jun

    2007-01-01

    This article concerns the delay-independent guaranteed-cost control problem via memoryless state feedback for a class of neutral-type systems with structural uncertainty and a given quadratic cost function. New delay-independent conditions for the existence of the guaranteed-cost controller are presented in the term of LMIs. An algorithm involving optimization is proposed to design a controller achieving an optimal guaranteed-cost, such that, the system can be stabilized for all admissible uncertainties. A numerical example is provided to illustrate the feasibility of the proposed method.

  10. Non-fragile hybrid guaranteed cost control for a class of uncertain switched linear systems

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    This paper focuses on the problem of non-fragile hybrid guaranteed cost control for a class of uncertain switched linear systems. The controller gain to be designed is assumed to have additive gain variations. Based on multiple-Lyapunov function technique, the design of non-fragile hybrid guaranteed cost controllers is derived to make the corresponding closed-loop system asymptotically stable for all admissible uncertainties. Furthermore, a convex optimization approach with LMIs constraints is introduced to select the optimal non-fragile guaranteed cost controllers. Finally, a simulation example illustrates the effectiveness of the proposed approach.

  11. ROBUST STABILITY WITH GUARANTEEING COST FOR DISCRETE TIME-DELAY SYSTEMS WITH NONLINEAR PERTURBATION

    Institute of Scientific and Technical Information of China (English)

    JIA Xinchun; ZHENG Nanning; LIU Yuehu

    2005-01-01

    The problems of robust stability and robust stability with a guaranteeing cost for discrete time-delay systems with nonlinear perturbation are discussed. A sufficient criterion for robust stability is established in an LMI framework and a linear convex optimization problem with LMI constraints for computing maximal perturbation bound is proposed. Meanwhile, a sufficient criterion for robust stability with a guaranteeing cost for such systems is obtained, and an optimal procedure for decreasing the value of guaranteeing cost is put forward. Two examples are used to illustrate the efficiency of the results.

  12. Staphylococcus aureus Peptidoglycan Tertiary Structure from Carbon-13 Spin Diffusion

    OpenAIRE

    Sharif, Shasad; Singh, Manmilan; Kim, Sung Joon; Schaefer,Jacob

    2009-01-01

    The cell-wall peptidoglycan of Staphylococcus aureus is a heterogeneous, highly cross-linked polymer of unknown tertiary structure. We have partially characterized this structure by measuring spin diffusion from 13C labels in pentaglycyl cross-linking segments to natural-abundance 13C in the surrounding intact cell walls. The measurements were performed using a version of centerband-only detection of exchange (CODEX). The cell walls were isolated from S. aureus grown in media containing [1-13...

  13. S. aureus bacteria : a new target of serum calcification activity

    OpenAIRE

    Dy, Diane Jazmin

    2009-01-01

    Staphylococcus aureus are gram- positive bacteria that cause skin and soft tissue infections. The continual incidence of infection is of great concern especially with the advent of methicillin resistant S. aureus (MRSA). Continued investigation on mechanisms our body uses to fight bacterial infection is vital. Our study suggests that the body takes advantage of a mechanism that mineralizes type-I collagen of bone and tendon to also mineralize bacteria. Serum driven bacterial mineralization ma...

  14. Comparative Pharmacodynamics of Gentamicin against Staphylococcus aureus and Pseudomonas aeruginosa†

    OpenAIRE

    Tam, Vincent H.; Kabbara, Samer; Vo, Giao; Schilling, Amy N.; Coyle, Elizabeth A.

    2006-01-01

    Aminoglycosides are often used to treat severe infections with gram-positive organisms. Previous studies have shown concentration-dependent killing by aminoglycosides of gram-negative bacteria, but limited data are available for gram-positive bacteria. We compared the in vitro pharmacodynamics of gentamicin against Staphylococcus aureus and Pseudomonas aeruginosa. Five S. aureus strains were examined (ATCC 29213 and four clinical isolates). Time-kill studies (TKS) in duplicate (baseline inocu...

  15. Role of Monocytes in Experimental Staphylococcus aureus Endocarditis

    OpenAIRE

    Veltrop, Marcel H. A. M.; Bancsi, Maurice J. L. M. F.; Bertina, Rogier M.; Thompson, Jan

    2000-01-01

    In the pathogenesis of bacterial endocarditis (BE), the clotting system plays a cardinal role in the formation and maintenance of the endocardial vegetations. The extrinsic pathway is involved in the activation of the coagulation pathway with tissue factor (TF) as the key protein. Staphylococcus aureus is a frequently isolated bacterium from patients with BE. We therefore investigated whether S. aureus can induce TF activity (TFA) on fibrin-adherent monocytes, used as an in vitro model of BE....

  16. Vancomycin Resistance Pattern of Staphylococcus Aureus among Clinical Samples

    OpenAIRE

    S Saadat; K Solhjoo; A. Kazemi; Erfanian, S. (MSc); Ashrafian, F. (MSc)

    2014-01-01

    Background and Objective: Vancomycin is used for treatment of methicillin-resistant S. Aureus (MRSA) infections; therefore, resistance to this antibiotic is increasing. We aimed to determine the antibiotic resistance pattern and frequency of vancomycin resistant S. Areas (VRSA) strains isolated from clinical samples. Material and Methods: In this cross-sectional study, 100 S. Aureus isolates collected from hospitals in Shiraz during six months, 2012, were identified by biochemical, microbiolo...

  17. AKTIVITAS ANTIBAKTERI KITOSAN TERHADAP BAKTERI S.aureus

    OpenAIRE

    Mardiyah Kurniasih; Dwi Kartika

    2009-01-01

    Chitosan is the N-deacetylated derivative of chitin. Chitosan is biodegradable, biocompatible and non-toxic. Chitosan is polycationic in acidic media and give antibacterial activity. In this paper, antibacterial activity of chitosan have been studied. Chitosan had been isolated from white shrimp. Antibacterial activity of chitosan solutions was examined against S. aureus The result showed that antimicrobial effect on S. aureus was strengthened as the choitosan concentrate decreased.

  18. Susceptibility of Staphylococcus aureus biofilms to reactive discharge gases

    OpenAIRE

    Traba, Christian; Liang, Jun F.

    2011-01-01

    Formation of bacterial biofilms at solid-liquid interfaces creates numerous problems in both industrial and biomedical sciences. In this study, the susceptibility of Staphylococcus aureus biofilms to discharge gas generated from plasma was tested. It was found that despite distinct chemical/physical properties, discharge gases from oxygen, nitrogen, and argon demonstrated very potent and almost the same anti-biofilm activity. The bacterial cells in S. aureus biofilms were killed (>99.9%) by d...

  19. Resistance to Antimicrobials Mediated by Efflux Pumps in Staphylococcus aureus

    OpenAIRE

    Isabel Couto; Leonard Amaral; José Melo-Cristino; Miguel Viveiros; Cláudia Palma; Elisabete Junqueira; Costa, Sofia S.

    2013-01-01

    Resistance mediated by efflux has been recognized in Staphylococcus aureus in the last few decades, although its clinical relevance has only been recognized recently. The existence of only a few studies on the individual and overall contribution of efflux to resistance phenotypes associated with the need of well-established methods to assess efflux activity in clinical isolates contributes greatly to the lack of solid knowledge of this mechanism in S. aureus. This study aims to provide inform...

  20. Methicillin resistance & inducible clindamycin resistance in Staphylococcus aureus

    OpenAIRE

    Soumyadeep Ghosh; Mandira Banerjee

    2016-01-01

    Background & objectives: Methicillin resistant Staphylococcus aureus (MRSA) isolates with inducible clindamycin resistance (iCR) are resistant to erythromycin and sensitive to clindamycin on routine testing and inducible clindamycin resistance can only be identified by D-test. This study was aimed to detect methicillin resistance and iCR among S. aureus isolates, effectiveness of some commonly used antibiotics and correlation between methicillin resistance and iCR. Methods: The present cro...

  1. Detection and characterization of mupirocin resistance in Staphylococcus aureus.

    OpenAIRE

    Janssen, D A; Zarins, L T; Schaberg, D R; Bradley, S. F.; Terpenning, M S; Kauffman, C A

    1993-01-01

    Fourteen mupirocin-resistant Staphylococcus aureus strains were isolated over 18 months; 12 exhibited low-level resistance, while two showed high-level resistance. Highly mupirocin-resistant strains contained a large plasmid which transferred mupirocin resistance to other S. aureus strains and to Staphylococcus epidermidis. This plasmid and pAM899-1, a self-transferable gentamicin resistance plasmid, have molecular and biologic similarities.

  2. Methicillin-Resistant Staphylococcus aureus Ocular Infection in Taiwan

    OpenAIRE

    Kang, Yu-Chuan; Hsiao, Ching-Hsi; Yeh, Lung-Kun; Ma, David H. K.; Chen, Phil Y. F.; Lin, Hsin-Chiung; Tan, Hsin-Yuan; Chen, Hung-Chi; Chen, Shin-Yi; Huang, Yhu-Chering

    2015-01-01

    Abstract Methicillin-resistant Staphylococcus aureus (MRSA) infection is an important public health issue. This observational study aimed to characterize clinical features, antibiotic susceptibility, and genotypes of ocular infections caused by MRSA based on the clinical and molecular definitions of community-associated (CA) and healthcare-associated (HA) strains. Fifty-nine patients with culture-proven S aureus ocular infection were enrolled from January 1, 2010 to December 31, 2011 at Chang...

  3. Resistance to Antimicrobials Mediated by Efflux Pumps in Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Isabel Couto

    2013-03-01

    Full Text Available Resistance mediated by efflux has been recognized in Staphylococcus aureus in the last few decades, although its clinical relevance has only been recognized recently. The existence of only a few studies on the individual and overall contribution of efflux to resistance phenotypes associated with the need of well-established methods to assess efflux activity in clinical isolates contributes greatly to the lack of solid knowledge of this mechanism in S. aureus. This study aims to provide information on approaches useful to the assessment and characterization of efflux activity, as well as contributing to our understanding of the role of efflux to phenotypes of antibiotic resistance and biocide tolerance in S. aureus clinical isolates. The results described show that efflux is an important contributor to fluoroquinolone resistance in S. aureus and suggest it as a major mechanism in the early stages of resistance development. We also show that efflux plays an important role on the reduced susceptibility to biocides in S. aureus, strengthening the importance of this long neglected resistance mechanism to the persistence and proliferation of antibiotic/biocide-resistant S. aureus in the hospital environment.

  4. Photoreactivation of ultraviolet-irradiation damage in Staphylococcus aureus

    International Nuclear Information System (INIS)

    This study reports the capacity of Staphylococcus aureus strain 7 - 8 to undergo photoenzymatic repair of UV-irradiation induced damage and compares it to the photoreactivation (PR) response of Escherichia coli strain B. Staphylococcus aureaus showed greater inhibition by UV irradiation than E. coli, consistent with its higher adenine and thymine content of DNA. Staphylococcus aureus showed an enhanced rate of photoreactivation with no lag in initiation of the PR response at low PR doses compared to E. coli. Maximum PR capacity of both cultures was about equal and occurred in cultures incubated at 23 - 250. The PR responses at 11 - 12 and 35 - 370 for S. aureus and E. coli differed although both were capable of PR at each of these temperatures. The PR response of E. coli was directly related to the dosage of PR light (J/m2); however, the photoenzymatic capacity of S. aureus was not directly responsive to continued decrease in light intensity. The capacity of S. aureus to undergo liquid holding recovery (LHR) occurred at 23 - 250 (not at 11 - 120 or 35 - 370), whereas E. coli underwent LHR at 11 - 120 and 23 - 250 but not at 35 - 370. The LHR response of S. aureus was somewhat more effective than E. coli and did not show the direct response to increased liquid-holding period as did E. coli. (author)

  5. Staphylococcus aureus – antimicrobial resistance and the immunocompromised child

    Directory of Open Access Journals (Sweden)

    McNeil JC

    2014-05-01

    Full Text Available J Chase McNeilDepartment of Pediatrics, Section of Infectious Diseases, Baylor College of Medicine, Houston, TX, USAAbstract: Children with immunocompromising conditions represent a unique group for the acquisition of antimicrobial resistant infections due to their frequent encounters with the health care system, need for empiric antimicrobials, and immune dysfunction. These infections are further complicated in that there is a relative paucity of literature on the clinical features and management of Staphylococcus aureus infections in immunocompromised children. The available literature on the clinical features, antimicrobial susceptibility, and management of S. aureus infections in immunocompromised children is reviewed. S. aureus infections in children with human immunodeficiency virus (HIV are associated with higher HIV viral loads and a greater degree of CD4 T-cell suppression. In addition, staphylococcal infections in children with HIV often exhibit a multidrug resistant phenotype. Children with cancer have a high rate of S. aureus bacteremia and associated complications. Increased tolerance to antiseptics among staphylococcal isolates from pediatric oncology patients is an emerging area of research. The incidence of S. aureus infections among pediatric solid organ transplant recipients varies considerably by the organ transplanted; in general however, staphylococci figure prominently among infections in the early posttransplant period. Staphylococcal infections are also prominent pathogens among children with a number of immunodeficiencies, notably chronic granulomatous disease. Significant gaps in knowledge exist regarding the epidemiology and management of S. aureus infection in these vulnerable children.Keywords: pediatric, HIV, cancer, transplant

  6. Determining of antibiotic resistance profile inStaphylococcus aureus isolates

    Institute of Scientific and Technical Information of China (English)

    Hossein Motamedi; Hadis Mirzabeigi; Tahere Shirali

    2010-01-01

    Objective:To determine the pattern of antibiotic resistance amongStaphylococcus aureus (S. aureus) isolates from clinical specimens and to identify community-acquired methicillin-resistantStaphylococcus aureus(CA-MRSA)in specimens that have been collected from patients referring to one of the hospitals of Ahvaz.Methods:S. aureus isolates from a hospital in Ahvaz were screened for resistance to various antibiotics including methicillin. The susceptibility of the isolates was determined by Kirby-Bauer disc diffusion method. TheMRSA was also treated with ethidium bromide to find the origin of resistance.Results: Among the bacterial isolates, all of 11S. aureus were resistant to methicillin and cefixime,2 were resistant to ciprofloxacine,6 were resistant to tetracycline and the reminder were sensitive or intermediate to other antibiotics. The treated isolates were reminded resistant to methicillin and this suggested that the plasmid was not the origin of resistance in these isolates.Conclusions: These results showed that infection due toMRSA is widespread in Ahvaz and with respect to the spread of vancomycin resistance among MRSA and appearance of overwhelming infections. It is necessary to identify continuously the profile of antibiotic resistance amongS. aureus isolates in other regions and finding appropriate antibiotic for infection control and eradication.

  7. Bank Panics, Government Guarantees, and the Long‐Run Size of the Financial Sector: Evidence from Free‐Banking America

    OpenAIRE

    Chabot, Benjamin; Charles C. Moul

    2014-01-01

    Governments often attempt to increase the confidence of financial market participants by making implicit or explicit guarantees of uncertain credibility. Confidence in these guarantees presumably alters the size of the financial sector, but observing the long-run consequences of failed guarantees is difficult in the modern era. We look to America’s free-banking era and compare the consequences of a broken guarantee during the Indiana-centered Panic of 1854 to the Panic of 1857 in which guaran...

  8. Supply guarantee initiatives for nuclear fuel materials and services and their compatibility with the market

    International Nuclear Information System (INIS)

    This paper first discusses where and why those ideas for nuclear fuel supply assurance and guarantee mechanisms came out, and attempts to draw a comparison among the proposed schemes and thereby examine possible steps forward. (author)

  9. Risk-Taking, Agency Problem, and Small Business Loan Guarantee: An Application of Option Pricing Theory

    OpenAIRE

    Jacky C. So; Bharati, Rakesh; Crain, Susan

    2001-01-01

    We examine the relationship between the small business loan guarantee and the agency problem of small firms. We then recommend financial instruments or financial contracts that can minimize of eliminate the moral hazard problem.

  10. A Best Practice for Developing Availability Guarantee Language in Photovoltaic (PV) O&M Agreements.

    Energy Technology Data Exchange (ETDEWEB)

    Klise, Geoffrey Taylor [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Balfour, John [High Performance PV, Phoenix, AZ (United States)

    2015-11-01

    This document outlines the foundation for developing language that can be utilized in an Equipment Availability Guarantee, typically included in an O&M services agreement between a PV system or plant owner and an O&M services provider, or operator. Many of the current PV O&M service agreement Availability Guarantees are based on contracts used for traditional power generation, which create challenges for owners and operators due to the variable nature of grid-tied photovoltaic generating technologies. This report documents language used in early PV availability guarantees and presents best practices and equations that can be used to more openly communicate how the reliability of the PV system and plant equipment can be expressed in an availability guarantee. This work will improve the bankability of PV systems by providing greater transparency into the equipment reliability state to all parties involved in an O&M services contract.

  11. Investigating the effects of issuance of bank debt guarantee on profitability of banking industry

    Directory of Open Access Journals (Sweden)

    Reza Pirayesh

    2014-02-01

    Full Text Available This paper presents an empirical investigation to study the relationship between bank debt guarantee and profitability, increase in banks resources and customer retention. The proposed study has been implemented in one of Iranian banks located in city of Zanjan, Iran over the period of 10 years. The study has used Pearson correlation test to examine three hypotheses of the survey. The results indicate that there were positive and meaningful relationship between bank debt guarantee and profitability (r = 0.863, P-value = 0.001, a positive relationship between bank debt guarantee and bank resources (r = 0.708 with P-value = 0.015 and there was a positive and meaningful relationship between bank debt guarantee and customer retention (r = 0.252 with P-value = 0.001.

  12. 30 CFR 256.57 - Using a third-party guarantee instead of a bond.

    Science.gov (United States)

    2010-07-01

    ... partnership, joint venture, or syndicate, the indemnity agreement must: (i) Bind each partner or party who has... under your third-party guarantee, each partner is jointly and severally liable for compliance with...

  13. 7 CFR 1738.30 - Rural broadband access loans and loan guarantees.

    Science.gov (United States)

    2010-01-01

    ... includes commercial banks, trust companies, mortgage banking firms, insurance companies, and any other... be, will neither be paid first nor given any preference or priority over the Guaranteed Loan...

  14. Reliable fuzzy control with domain guaranteed cost for fuzzy systems with actuator failures

    Institute of Scientific and Technical Information of China (English)

    JIA Xinchun; ZHENG Nanning

    2004-01-01

    The reliable fuzzy control with guaranteed cost for T-S fuzzy systems with actuator failure is proposed in this paper. The cost function is a quadratic function with failure input. When the initial state of such systems is known, a design method of the reliable fuzzy controller with reliable guaranteed cost is presented, and the formula of the guaranteed cost is established. When the initial state of such systems is unknown but belongs to a known bounded closed domain, a notion of the reliable domain guaranteed cost (RDGC) for such systems is proposed. For two classes of initial state domain, polygon domain and ellipsoid domain, some design methods for reliable fuzzy controllers with the RDGC are provided. The efficiency of our design methods is finally verified by numerical design and simulation on the Rossler chaotic system.

  15. 7 CFR Appendix I to Subpart E of... - Instructions for Loan Guarantees for Drought and Disaster Relief

    Science.gov (United States)

    2010-01-01

    ...), accounts receivable, personal and corporate guarantees, and other existing and contingent assets, advice as... guaranteed is governed by 7 CFR Part 1980 Subpart E Appendix I and the Loan Note Guarantee (Drought and... in the applicable Subpart of Title 7 CFR Part 1980, and to future FmHA or its successor agency...

  16. Comparison of the guaranteed analysis with the measured nutrient composition of commercial pet foods

    OpenAIRE

    Hill, Richard C.; Choate, Christina J; Scott, Karen C.; Molenberghs, Geert

    2009-01-01

    Objective-To compare guaranteed and measured concentrations of nutrients in commercial pet foods. Design-Cross-sectional study. Sample Population-Annual inspection reports of pet food analyses from 5 states. Procedures-Guaranteed and measured concentrations of crude protein (CP), crude fat (CF), crude fiber (CFb), moisture, and ash in pet foods were compared. The concentration difference for each nutrient was compared among types of food, target species, target life stages, manufacturers, and...

  17. THE LETTER OF GUARANTEE FROM THE PERSPECTIVE OF THE NEW CIVIL CODE

    OpenAIRE

    LUMINITA TULEASCA

    2011-01-01

    The letter of guarantee is frequently used in the domestic and international commercial activity, first of all, for the safety offered for securing the contractual obligations without blocking the pecuniary funds and, second of all, due to the existing uniform international regulations that correspond to the needs supporting the occurrence of these types of autonomous securities.In Romania, the lack of legal policing of the letter of guarantee has created practical difficulties and offered ju...

  18. A Simple Method for Guaranteeing ECG Quality in Real-Time Wavelet Lossy Coding

    OpenAIRE

    Alesanco Álvaro; García José

    2007-01-01

    Guaranteeing ECG signal quality in wavelet lossy compression methods is essential for clinical acceptability of reconstructed signals. In this paper, we present a simple and efficient method for guaranteeing reconstruction quality measured using the new distortion index wavelet weighted PRD (WWPRD), which reflects in a more accurate way the real clinical distortion of the compressed signal. The method is based on the wavelet transform and its subsequent coding using the set partitioning in h...

  19. The role of guarantees in pension plan design: Pension saving decisions

    OpenAIRE

    Lourenço, Ana

    2016-01-01

    This study centers on the assessment of psychological value of guarantees in pension products and the behavior biases associated with choice. When a guarantee on a product increases from 99% to 99,5% less than half of respondents show willingness to pay in contrast with 73% when going from 99,5% to 100%. Out of 105 respondents, 55 show that their choices concerning pension products are inconsistent with classic utility theory. Financial background proves insignificant thus pointing to behavio...

  20. Exploring the Impacts of Service Guarantee Strategy in the Casual Restaurant Sector

    OpenAIRE

    Lee, Kyuho

    2006-01-01

    This study examined the impacts of service guarantees in terms of consumers' perceived risks, loyalty, and word-of-mouth intent in the casual dining restaurant segment. In addition, the study investigated what type of service guarantee consumers preferred. A total of seven research hypotheses were developed on the basis of relevant literature review. In an effort to test the research hypotheses, a scenario method was developed. A total of seven research scenarios were created based on the Vir...

  1. Control the guaranteed search of moving objects at minimum expense of light energy

    OpenAIRE

    Avetisyan V.V.; Martirosyan S. R.

    2008-01-01

    An algorithm of control manipulator gripper movement in given light conditions with optimal, minimum expense of light energy has been developed, provides the location of the searched object within the planned time frame. It has been found and justified through digital calculations that optimal processes of guaranteed search are possible which require same volume of light energy expense, but, however, in one case the optimal guaranteed search is implemented quickly and needs a great light sour...

  2. An Active Queue Management for QoS Guarantee of the High Priority Service Class

    Science.gov (United States)

    Kim, Hyun Jong; Shim, Jae Chan; Kim, Hwa-Suk; Cho, Kee Seong; Choi, Seong Gon

    In this paper, we propose the active queue management mechanism (Active-WRED) for guaranteeing the quality of the high priority service class (VoIP or IPTV) in the multi-class traffic service environment. In the congestion situation, this mechanism increases the drop probability of the low priority traffic and reduces the drop probability of the high priority traffic; therefore it can guarantee the quality of the high priority service class from the poor quality by the packet loss.

  3. Guaranteed cost control for uncertain stochastic fuzzy systems with time delay

    Institute of Scientific and Technical Information of China (English)

    ZHANG Huaguang; WANG Yingchun

    2007-01-01

    This paper studies a delay-dependent guaranteed cost control problem for a class of uncertain nonlinear stochastic systems with time delay represented by the Takagi-Sugeno (T-S) fuzzy model with uncertain parameters. The descriptor system method and Gu's inequality are employed to obtain delay-dependent sufficient conditions such that the closed-loop system is asymptotically stable with a certain guaranteed cost control performance. The effectiveness of the proposed method is shown by a simulation example.

  4. Guarantees of the Legality of Administration and Administrative Procedure in Hungary

    OpenAIRE

    Anita Paulovics

    2011-01-01

    The legality of administration is a fundamental constitutional requirement in a constitutional government. For this reason, modern laws regulating administrative procedure provide for different guarantees of the legality of administration. The Administrative Procedure Act in force in Hungary (Act No. CXL. of 2004) contains a wide range of rules aimed at guaranteeing the legality of the administrative action in all phases of the administrative procedure. The most important of them is the judic...

  5. Nonfragile Guaranteed Cost Control and Optimization for Interconnected Systems of Neutral Type

    OpenAIRE

    Qingling Zhang; Heli Hu; Dan Zhao

    2013-01-01

    The design and optimization problems of the nonfragile guaranteed cost control are investigated for a class of interconnected systems of neutral type. A novel scheme, viewing the interconnections with time-varying delays as effective information but not disturbances, is developed to decrease the conservatism. Many techniques on decomposing and magnifying the matrices are utilized to obtain the guaranteed cost of the considered system. Also, an algorithm is proposed to solve the nonlinear prob...

  6. Exponential Stabilization of Impulsive Switched Systems with Time Delays Using Guaranteed Cost Control

    OpenAIRE

    2014-01-01

    This paper investigates the stabilization problem for impulsive switched systems with time delays. First, exponential stability criteria of the delayed impulsive switched systems are established by use of the Lyapunov-Krasovskii functional method. Based on these results, sufficient conditions for the existence of a guaranteed cost control are also given. Subject to these sufficient conditions, the closed-loop impulsive switched system under the guaranteed cost control law will be exponentiall...

  7. PREMIUM RATES FOR YIELD GUARANTEE AND INCOME PROTECTION CROP INSURANCE FOR GEORGIA AND SOUTH CAROLINA PEACHES

    OpenAIRE

    Miller, Stephen E.; Kahl, Kandice H.; Rathwell, P. James

    1999-01-01

    We estimate actuarially sound premium rates for individual yield guarantee and income protection crop insurance products for Georgia and South Carolina peaches. In most cases, the premium rate for an income protection product equals or exceeds the premium rate for an individual yield guarantee product for a given coverage level and average farm yield. Although the premium rates for the two products differ in a statistical sense, they do not appear to differ in an economic sense except at high...

  8. The Implementation of Service Guarantees in China Service and Manufacturing Industries

    OpenAIRE

    Tang, Lan

    2011-01-01

    With the development of economy in the world, people would like to spend more money to enjoy their life. In this way, service industry becomes more popular than manufacturing industry. In addition, although the main business of a company is production, it will provide additional services, such as after-sales services, to increase its products’ selling volume. At the same time, a good service quality should be improved and guaranteed by applying service guarantee. However, China is a developin...

  9. Delay-dependent guaranteed cost control for uncertain systems with both state and input delays

    Institute of Scientific and Technical Information of China (English)

    Xuanfang YANG; Wuhua CHEN; Huajing FANG

    2004-01-01

    This paper considers the guaranteed cost control problem for a class of uncertain linear systems with both state and input delays.By representing the time-delay system in the descriptor system form and using a recent result on bounding of cross products of vectors,we obtain new delay-dependent sufficient conditions for the existence of the guaranteed cost controller in terms of linear matrix inequalities.Two examples are presented which show the effectiveness of our approach.

  10. Is biomass always a renewable fuel as guaranteed?

    International Nuclear Information System (INIS)

    alternative electric energy must be energy which is not produced from oil shale. TheLong-Term Development Plan for the Estonian Fuel and Energy Sector includes such misleading terms as peat, wood and renewable energy resources, biofuels and peat. However, there are also quite correct phrases such as peat, wood and other renewable natural resources, as wood and other biofuels are renewable natural resources. As a rule, wood and wood waste, energy cultures, agricultural, woodpulp and paper industry waste, as well as solid animal breeding and household waste, and also peat are classified as biomass in many countries. Biomass seems to be a more convenient term instead of renewable biofuel. In most statements it is with no doubt classified as renewable resource, while biofuels are not because fossil fuels are also biofuels (or fuels of biological origin) and they are renewable only to a very limited extent. Fossil fuels originated in sapropel and peat, oil and natural gas from dispersed organic matter, too. So, their recovery depends on the speed of accumulation of organic carbon in recent sediments. The recovery should not be a question of what somebody believes in or not. It must not be a 'religious' question but a purely scientific and methodological issue. There is only one condition when a fuel (or biomass as one type of biofuel) is renewable: biomass or biofuel may be considered renewable if they are replenished at a rate which is comparable to the rate at which they are consumed in a country or part of it (on an island, for example). The overused amount is a non-renewable biomass or biofuel. So, the replenishment of biomass or biofuel is not guaranteed. Biomass and other biofuels, including fossil ones, are products of the activity of the sun. There are also other, non-fuel types of energy such as water and wind energy, as well as solar and geothermal energy. They are also considered renewable energy resources as is biomass. All these types of energy (except, may be, the

  11. Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) at ambient freshwater beaches

    Science.gov (United States)

    Fogarty, Lisa R.; Haack, Sheridan K.; Johnson, Heather E.; Brennan, Angela K.; Isaacs, Natasha M.; Spencer, Chelsea

    2015-01-01

    Methicillin-resistant Staphylococcus aureus (MRSA) are a threat to human health worldwide, and although detected at marine beaches, they have been largely unstudied at freshwater beaches. Genes indicating S. aureus (SA; femA) and methicillin resistance (mecA) were detected at 11 and 12 of 13 US Great Lakes beaches and in 18% or 27% of 287 recreational water samples, respectively. Eight beaches had mecA + femA (potential MRSA) detections. During an intensive study, higher bather numbers, staphylococci concentrations, and femA detections were found in samples collected after noon than before noon. Local population density, beach cloud cover, and beach wave height were significantly correlated with SA or MRSA detection frequency. The Panton-Valentine leukocidin gene, associated with community-acquired MRSA, was detected in 12 out of 27 potential MRSA samples. The femA gene was detected less frequently at beaches that met US enterococci criteria or EU enterococci ‘excellent’ recreational water quality, but was not related to Escherichia coli-defined criteria. Escherichia coli is often the only indicator used to determine water quality at US beaches, given the economic and healthcare burden that can be associated with infections caused by SA and MRSA, monitoring of recreational waters for non-fecal bacteria such as staphylococci and/or SA may be warranted.

  12. Staphylococcus aureus small colony variants in diabetic foot infections.

    Science.gov (United States)

    Cervantes-García, Estrella; García-Gonzalez, Rafael; Reyes-Torres, Angélica; Resendiz-Albor, Aldo Arturo; Salazar-Schettino, Paz María

    2015-01-01

    Background : Staphylococcus aureus (S. aureus) is one of the major pathogens causing chronic infections. The ability of S. aureus to acquire resistance to a diverse range of antimicrobial compounds results in limited treatment options, particularly in methicillin-resistant S. aureus (MRSA). A mechanism by which S. aureus develops reduced susceptibility to antimicrobials is through the formation of small colony variants (SCVs). Infections by SCVs of S. aureus are an upcoming problem due to difficulties in laboratory diagnosis and resistance to antimicrobial therapy. Methods : A prospective study was performed on 120 patients diagnosed with both type 2 diabetes mellitus and infected diabetic foot ulcers. The study was carried out from July 2012 to December 2013 in Hospital General de Mexico. The samples were cultured in blood agar, mannitol salt agar, and MacConkey agar media, and incubated at 37°C in aerobic conditions. Results : We describe the first known cases of diabetic foot infections caused by MRSA-SCVs in patients diagnosed with type 2 diabetes mellitus and infected diabetic foot ulcers. In all of our cases, the patients had not received any form of gentamicin therapy. Conclusions : The antibiotic therapy commonly used in diabetic patients with infected diabetic foot ulcers fails in the case of MRSA-SCVs because the intracellular location protects S. aureus-SCVs from the host's defenses and also helps them resist antibiotics. The cases studied in this article add to the spectrum of persistent and relapsing infections attributed to MRSA-SCVs and emphasizes that these variants may also play a relevant role in diabetic foot infections. PMID:25787018

  13. Staphylococcus aureus small colony variants in diabetic foot infections

    Directory of Open Access Journals (Sweden)

    Estrella Cervantes-García

    2015-03-01

    Full Text Available Background: Staphylococcus aureus (S. aureus is one of the major pathogens causing chronic infections. The ability of S. aureus to acquire resistance to a diverse range of antimicrobial compounds results in limited treatment options, particularly in methicillin-resistant S. aureus (MRSA. A mechanism by which S. aureus develops reduced susceptibility to antimicrobials is through the formation of small colony variants (SCVs. Infections by SCVs of S. aureus are an upcoming problem due to difficulties in laboratory diagnosis and resistance to antimicrobial therapy. Methods: A prospective study was performed on 120 patients diagnosed with both type 2 diabetes mellitus and infected diabetic foot ulcers. The study was carried out from July 2012 to December 2013 in Hospital General de Mexico. The samples were cultured in blood agar, mannitol salt agar, and MacConkey agar media, and incubated at 37°C in aerobic conditions. Results: We describe the first known cases of diabetic foot infections caused by MRSA-SCVs in patients diagnosed with type 2 diabetes mellitus and infected diabetic foot ulcers. In all of our cases, the patients had not received any form of gentamicin therapy. Conclusions: The antibiotic therapy commonly used in diabetic patients with infected diabetic foot ulcers fails in the case of MRSA-SCVs because the intracellular location protects S. aureus-SCVs from the host's defenses and also helps them resist antibiotics. The cases studied in this article add to the spectrum of persistent and relapsing infections attributed to MRSA-SCVs and emphasizes that these variants may also play a relevant role in diabetic foot infections.

  14. PRICING AND ASSESSING UNIT-LINKED INSURANCE CONTRACTS WITH INVESTMENT GUARANTEES

    Directory of Open Access Journals (Sweden)

    Ciumas Cristina

    2014-07-01

    Full Text Available One of the most interesting life insurance products to have emerged in recent years in the Romanian insurance market has been the unit-linked contract. Unit-linked insurance products are life insurance policies with investment component. A unit-linked life insurance has two important components: protection and investment. The protection component refers to the insured sum in case of the occurrence of insured risks and the investment component refers to the policyholders’ account that represents the present value of the units from the chosen investment funds. Due to the financial instability caused by the Global Crisis and the amplification of market competitiveness, insurers from international markets have started to incorporate guarantees in unit-linked products. So a unit- linked life insurance policy with an asset value guarantee is an insurance policy whose benefit payable on death or at maturity consists of the greater of some guaranteed amount and the value of the units from the investment funds. One of the most challenging issues concerns the pricing of minimum death benefit and maturity benefit guarantees and the establishing of proper reserves for these guarantees. Insurers granting guarantees of this type must estimate the cost and include the cost in the premium. An important component of the activity carried out by the insurance companies is the investment of the premiums paid by policyholders in various types of assets, in order to obtain higher yields than those guaranteed by the insurance contracts, while providing the necessary liquidity for the payment of insurance claims in case of occurrence of the assumed risks. So the guaranteed benefits can be broadly matched or immunized with various types of financial assets, especially with fixed-interest instruments. According to Romanian legislation which regulates the unit-linked life insurance market, unit-linked life insurance contracts pass most of the investment risk to the

  15. A cohort study of the Copenhagen CF Centre eradication strategy against Staphylococcus aureus in patients with CF

    DEFF Research Database (Denmark)

    Dalbøge, Christina Schjellerup; Pressler, Tacjana; Høiby, Niels;

    2013-01-01

    Staphylococcus aureus is an important pathogen in CF. Centre prevalence of intermittent colonization and chronic S. aureus infections and the effectiveness of an anti-S. aureus eradication strategy was assessed....

  16. Radioimmunoassays for protein A of Staphylococcus aureus

    International Nuclear Information System (INIS)

    Radioimmunoassays have been developed that can detect nanogram amounts of protein A (SpA), a product generated by Staphylococcus aureus that binds selectively to the Fc region of IgG from most mammalian species. Competition assays for fluid phase SpA utilize antibodies produced in chickens, 125I-labeled SpA as the tracer molecule, and either F(ab')2 fragments of rabbit IgG anti-chicken IgG or 40% ammonium sulfate as the precipitating agent to separate antigen-antibody complexes from free antigen. The double antibody assay could be carried out in serum from species that form only soluble complexes with SpA (e.g., rabbit), that react poorly with SpA (e.g., rat) or under appropriate conditions in serum from species (e.g., dog) that show high reactivity with SpA and form precipitating complexes. Chicken antibodies prepared by affinity chromatography on SpA-Sepharose and labeled with 125I were used in a direct binding assay for SpA present either on the cell wall of Cowan strain I or Wood 46 bacteria, in insoluble complexes prepared from SpA and whole serum or purified IgG, or in C1q binding complexes that were formed by passage of serum from normal or tumor bearing humans or dogs over SpA-collodion charcoal. Since both types of assays could detect SpA even in the presence of serum or IgG, they offer advantages over other techniques in which the SpA-Fc interaction may interfere. (Auth.)

  17. A systematic review of animal models for Staphylococcus aureus osteomyelitis

    Directory of Open Access Journals (Sweden)

    W Reizner

    2014-03-01

    Full Text Available Staphylococcus aureus (S. aureus osteomyelitis is a significant complication for orthopaedic patients undergoing surgery, particularly with fracture fixation and arthroplasty. Given the difficulty in studying S. aureus infections in human subjects, animal models serve an integral role in exploring the pathogenesis of osteomyelitis, and aid in determining the efficacy of prophylactic and therapeutic treatments. Animal models should mimic the clinical scenarios seen in patients as closely as possible to permit the experimental results to be translated to the corresponding clinical care. To help understand existing animal models of S. aureus, we conducted a systematic search of PubMed and Ovid MEDLINE to identify in vivo animal experiments that have investigated the management of S. aureus osteomyelitis in the context of fractures and metallic implants. In this review, experimental studies are categorised by animal species and are further classified by the setting of the infection. Study methods are summarised and the relevant advantages and disadvantages of each species and model are discussed. While no ideal animal model exists, the understanding of a model’s strengths and limitations should assist clinicians and researchers to appropriately select an animal model to translate the conclusions to the clinical setting.

  18. Methicillin-resistant Staphylococcus aureus in central Iowa wildlife.

    Science.gov (United States)

    Wardyn, Shylo E; Kauffman, Lin K; Smith, Tara C

    2012-10-01

    Livestock and pets have been identified as carriers of Staphylococcus aureus; however, the role of wild animals as a reservoir of S. aureus strains has not yet been examined. We conducted a pilot study to determine the prevalence of methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in 37 species of wild animals rehabilitated at a university clinic. Nasal, wing, wound, and cloacal swabs were collected. Of 114 animals, seven (6.1%) were MSSA-positive and three (2.6%) were MRSA-positive. The MRSA isolates were obtained from two eastern cottontail rabbits (Sylvilagus floridanus) and a Lesser Yellowlegs (Tringa flavipes), a migratory shorebird. Antibiotic resistance testing of the MRSA isolates revealed that two were additionally resistant to tetracycline and erythromycin, and the third isolate was also resistant to erythromycin, clindamycin, and levofloxacin. All three isolates were positive for the Panton-Valentine leukocidin (PVL) gene. Sequence typing of the staphylococcal protein A (spa) region revealed one MRSA isolate to be t002, whereas the other two MRSA isolates were found to be t008. Our results suggest that S. aureus, including MRSA, is being carried by wild animals, although at a low prevalence with the limited number of animals tested. Additional studies are needed to determine how this may impact human health. PMID:23060511

  19. Crystal Violet and XTT Assays on Staphylococcus aureus Biofilm Quantification.

    Science.gov (United States)

    Xu, Zhenbo; Liang, Yanrui; Lin, Shiqi; Chen, Dingqiang; Li, Bing; Li, Lin; Deng, Yang

    2016-10-01

    Staphylococcus aureus (S. Aureus) is a common food-borne pathogenic microorganism. Biofilm formation remains the major obstruction for bacterial elimination. The study aims at providing a basis for determining S. aureus biofilm formation. 257 clinical samples of S. aureus isolates were identified by routine analysis and multiplex PCR detection and found to contain 227 MRSA, 16 MSSA, 11 MRCNS, and 3 MSCNS strains. Two assays for quantification of S. aureus biofilm formation, the crystal violet (CV) assay and the XTT (tetrazolium salt reduction) assay, were optimized, evaluated, and further compared. In CV assay, most isolates formed weak biofilm 74.3 %), while the rest formed moderate biofilm (23.3 %) or strong biofilm (2.3 %). However, most isolates in XTT assay showed weak metabolic activity (77.0 %), while the rest showed moderate metabolic activity (17.9 %) or high metabolic activity (5.1 %). In this study, we found a distinct strain-to-strain dissimilarity in terms of both biomass formation and metabolic activity, and it was concluded from this study that two assays were mutual complementation rather than being comparison. PMID:27324342

  20. Enterotoxin production by Staphylococcus aureus isolated from mastitic cows.

    Science.gov (United States)

    Cenci-Goga, B T; Karama, M; Rossitto, P V; Morgante, R A; Cullor, J S

    2003-09-01

    Staphylococcus aureus is an important cause of mastitis in cows. The ability of S. aureus strains to produce one or more enterotoxins in milk and dairy products is linked to staphylococcal food poisoning. To determine whether staphylococci causing bovine mastitis could cause human foodborne intoxication, the production of staphylococcal enterotoxins A through D (SEA, SEB, SEC, and SED) by 160 S. aureus isolates was evaluated with the use of a reverse passive latex agglutination enterotoxin kit. All S. aureus strains were isolated over a 9-month period from 2,343 routine submissions of a composite quarter collection of individual mastitic cows at 18 dairy farms in the San Joaquin Valley in California. Prior to enterotoxin detection, isolates were grown by a method that enhances the in vitro synthesis of enterotoxin. Twenty-two of 160 S. aureus isolates produced enterotoxin. Seven produced SEC, 12 produced SED, and 3 produced both SEC and SED. None of the isolates produced SEA or SEB. PMID:14503727

  1. Prevalence of Salmonella and Staphylococcus aureus in chorizo and longaniza

    Directory of Open Access Journals (Sweden)

    Refugio Torres-Vitela

    2011-12-01

    Full Text Available Epidemiological research in developed and developing countries, had found meat products as the principal cause for foodbourne diseases. In addition, Salmonella and Staphyococcus aureus are well known pathogens for their mayor impact in public health. The objective for the present study consisted on determinate the sanitary quality from chorizo and longaniza samples from several butcheries in Guadalajara, Jalisco, Mexico. Samples of chorizo (50 and longaniza (50 were obtained from different points in Guadalajara metropolis. Presence of Salmonella and recounts for S. aureus were tested in 25 g samples. Procedure was followed according Mexican NOM 145-SSA1-1995 methods. In chorizo, 18 samples were positive to Salmonella. The count of S. aureus showed a mean of 24,600 UFC/g. On the other hand, 24 samples of longaniza were positive to Salmonella spp. In this case, the mean of S. aureus was 7,800 UFC/g. The serotypes of Salmonella spp were: Derby (30%, Adelaile (17%, Azteca (15%, Infantis (15%, Muenster(10% y Anatum (13 %. The high positivity of Salmonella spp. and S. aureus is a potential hazard to consumers.

  2. On QoS guarantee in MPLS network with software deadline awareness

    Science.gov (United States)

    Gao, Yu; Jin, Yaohui; Cheng, Hong; Yu, Yingdi; Sun, Weiqiang; Guo, Wei; Hu, Weisheng

    2007-11-01

    With the increasing number of internet users, more and more real-time services are added into the network, such as IPTV, online-video, Voice over IP, etc. As we know, throughput is a highly important performance criterion for scheduling algorithms of networks. Besides throughput, these growing real-time services are very sensitive to delay variance termed as jitter. Therefore, high performance scheduling algorithm should achieve good performance in terms of throughput, delay, jitter and fairness together. Scheduling algorithm with deadline-awareness employed in packet switching plays a key role in QoS guarantees. Although traditional hard-deadline can provide guarantee on in-node delay-deadline criteria, the end-to-end delay-deadline criteria cannot be guaranteed due to the characteristic of hard-deadline, while the delay-deadline guaranteed throughput is low. Thus, soft-deadline scheduling algorithm has been proposed. In this paper, we investigate and compare the classical hard-deadline and soft-deadline switching algorithm. A new scheme "Bonus System" is proposed in order to realize the idea of soft-deadline switching algorithm. Bonus System based on soft-deadline is compared with classical Early-Deadline First scheme based on hard-deadline, which shows that Bonus System has better performance in End-to-End deadline guarantee.

  3. Occurrence of Multidrug Resistant Staphylococcus aureus in horses in Malaysia

    Directory of Open Access Journals (Sweden)

    Z. Zunita

    2008-12-01

    Full Text Available A total of 22 Staphylococcus aureus were isolated from 50 samples from 8 stable horses. They are positive in the catalase and coagulase tests. Upon testing the cultures with SLIDEX test kit all formed agglutination within a few seconds, confirming they are of S. aureus. When cultured onto MSA, all isolates formed yellow colonies. However, none of the isolates produced blue colonies on ORSAB indicating that there were no MRSA among the S. aureus. There were 13 isolates which were multiresistant. Eleven are resistant to eight out of ten antibiotics tested. All these isolates were found to originate from stable G. One isolate is resistant to 5 antibiotics while another one isolate is resistant to 3 antibiotics. The rest of the isolates are not multiresistant to the antibiotics tested. [Veterinary World 2008; 1(6.000: 165-167

  4. Risk factors for Staphylococcus aureus nasal colonization in Danish middle-aged and elderly twins

    DEFF Research Database (Denmark)

    Andersen, P S; Larsen, Lisbeth Aagaard; Fowler, V G;

    2013-01-01

    Staphylococcus aureus is a human commensal bacterium found in the nasal cavity and other body sites. Identifying risk factors for S. aureus nasal carriage is of interest, as nasal carriage is a risk factor for subsequent invasive infection. We recently investigated the influence of host genetics...... on S. aureus carriage in Danish middle-aged and elderly twins, which indicated no significant heritability that could account for the observed S. aureus carriage. In the present study, we performed a questionnaire-based study of S. aureus colonization on the same cohort of 2,196 Danish middle......-aged and elderly twins to identify specific risk factors for S. aureus nasal colonization, including analyzing the paired twins (n = 478) that were discordant for S. aureus colonization. We found associations between risk factors and S. aureus nasal colonization among middle-aged and elderly twins, including age...

  5. Draft Genome Sequence of Methicillin-Sensitive Staphylococcus aureus ATCC 29213

    OpenAIRE

    Soni, Isha; Chakrapani, Harinath; Chopra, Sidharth

    2015-01-01

    Staphylococcus aureus subsp. aureus ATCC 29213 is one of the most commonly used strains in drug discovery research and for quality control. We report the completed draft genome sequence for the strain.

  6. Quality control of direct molecular diagnostics for methicillin-resistant Staphylococcus aureus.

    NARCIS (Netherlands)

    A.F. van Belkum (Alex); H.G.M. Niesters (Bert); W.G. MacKay (William); W.B. van Leeuwen (Willem)

    2007-01-01

    textabstractTen samples containing various amounts of methicillin-resistant Staphylococcus aureus (MRSA), methicillin-susceptible S. aureus, methicillin-resistant Staphylococcus epidermidis (MRSE), and combinations thereof were distributed to 51 laboratories for molecular diagnostics testing. Sample

  7. Quality control of direct molecular diagnostics for methicillin-resistant Staphylococcus aureus

    NARCIS (Netherlands)

    van Belkum, Alex; Niesters, Hubert G M; MacKay, William G; van Leeuwen, Willem B

    2007-01-01

    Ten samples containing various amounts of methicillin-resistant Staphylococcus aureus (MRSA), methicillin-susceptible S. aureus, methicillin-resistant Staphylococcus epidermidis (MRSE), and combinations thereof were distributed to 51 laboratories for molecular diagnostics testing. Samples containing

  8. The Significance of Nasal Carriage of Staphylococcus Aureus and the Incidence of Postoperative Wound Infection

    NARCIS (Netherlands)

    R.P. Wenzel (Richard); T. M. Perl

    1995-01-01

    textabstractStaphylococcus aureus infections are associated with considerable morbidity and, in certain situations, mortality. The association between the nasal carriage of S. aureus and subsequent infection has been comprehensively established in a variety of clinical settings, in particular, patie

  9. Prevalence of infective endocarditis in patients with Staphylococcus aureus bacteraemia: the value of screening with echocardiography

    DEFF Research Database (Denmark)

    Rasmussen, Rasmus V; Høst, Ulla; Arpi, Magnus;

    2011-01-01

    Aims Staphylococcus aureus infective endocarditis (IE) is a critical medical condition associated with a high morbidity and mortality. In the present study, we prospectively evaluated the importance of screening with echocardiography in an unselected S. aureus bacteraemia (SAB) population. Methods...

  10. Execution of Fiduciary Guarantee Under Law No. 42 of 1999 on Fiduciary Guarantee (A Socio-Juridical Analysis to Anticipate Its Effectiveness

    Directory of Open Access Journals (Sweden)

    Arie Sukanti

    2013-09-01

    Full Text Available Fiduciary Guarantee (Fiduciary Law which approved by the House of Representatives of the Republic of Indonesia (DPR RI on September 9, 1999 has accommodate the public needs to help business activities and to provide legal certainty to the interested parties. With the increase in the development activities and the needs for funding, a majority of funds are needed to meet the lending and borrowing activities that require protection for the lender and the borrower through a guarantee institution that can provide legal certainty and protection to the lender or the borrower. Viewed from the current lending practices, there is a difficulty on the part of the Fiduciary Guarantee to conduct the fiduciary execution if the Fiduciary Grantor defaults since in fact the goods being a fiduciary object are still in the possession of the Fiduciary Grantor or Debtor, then in line with the provisions of article 1977 of the Indonesian Civil Code, known as the principle of bezit geldt als volkomen titel.

  11. Characterization of Staphylococcus aureus infections in children with Down syndrome.

    Science.gov (United States)

    Johnston, Jeffrey N; Kaplan, Sheldon L; Mason, Edward O; Hulten, Kristina G

    2015-11-01

    Staphylococcus aureus infections in the Down syndrome (DS) population have not been well characterized. This study determined clinical and molecular characteristics of S. aureus infections in children with DS followed at Texas Children's Hospital (TCH), from 2001 to 2011. Patients were retrospectively identified from an ongoing S. aureus surveillance study. Medical records were reviewed. Isolates were characterized by antimicrobial susceptibility, pulsed-field gel electrophoresis patterns, and detection of PVL genes (pvl), mupA (high-level mupirocin resistance gene), smr (chlorhexidine resistance conferring gene), and Staphylococcal Chromosomal Cassette mec (SCCmec) type. Twenty-six patients with DS had a total of 34 S. aureus infections (8 recurrent); 61% were MRSA. DS patients represented 16.8 per 10,000 community onset S. aureus infections seen at TCH. Among 26 initial infections 17 were skin and soft tissue (SSTI), 7 were outer or middle ear and 2 were invasive infections. Seventeen patients were hospitalized. Thirteen (65%) of 20 available isolates were USA300, 14 were pvl+, 5 were mupA+, and 8 were smr+. Five of 8 (63%) recurrent infections were ear infections. All 4 recurrent ear isolates available for study were smr+, ciprofloxacin non-susceptible and treated with ciprofloxacin otic drops. S. aureus infections among patients with DS were similar in presentation to other patient groups, except for a greater proportion being associated with ear infections. Seventy percent of ear fluid isolates carried antiseptic and fluoroquinolone resistance genes. A study of a greater number of DS patients is warranted to further explore these findings. PMID:26386776

  12. Highly sensitive detection of Staphylococcus aureus directly from patient blood.

    Directory of Open Access Journals (Sweden)

    Padmapriya P Banada

    Full Text Available BACKGROUND: Rapid detection of bloodstream infections (BSIs can be lifesaving. We investigated the sample processing and assay parameters necessary for highly-sensitive detection of bloodstream bacteria, using Staphylococcus aureus as a model pathogen and an automated fluidic sample processing-polymerase chain reaction (PCR platform as a model diagnostic system. METHODOLOGY/PRINCIPAL FINDINGS: We compared a short 128 bp amplicon hemi-nested PCR and a relatively shorter 79 bp amplicon nested PCR targeting the S. aureus nuc and sodA genes, respectively. The sodA nested assay showed an enhanced limit of detection (LOD of 5 genomic copies per reaction or 10 colony forming units (CFU per ml blood over 50 copies per reaction or 50 CFU/ml for the nuc assay. To establish optimal extraction protocols, we investigated the relative abundance of the bacteria in different components of the blood (white blood cells (WBCs, plasma or whole blood, using the above assays. The blood samples were obtained from the patients who were culture positive for S. aureus. Whole blood resulted in maximum PCR positives with sodA assay (90% positive as opposed to cell-associated bacteria (in WBCs (71% samples positive or free bacterial DNA in plasma (62.5% samples positive. Both the assays were further tested for direct detection of S. aureus in patient whole blood samples that were contemporaneous culture positive. S. aureus was detected in 40/45 of culture-positive patients (sensitivity 89%, 95% CI 0.75-0.96 and 0/59 negative controls with the sodA assay (specificity 100%, 95% CI 0.92-1. CONCLUSIONS: We have demonstrated a highly sensitive two-hour assay for detection of sepsis causing bacteria like S. aureus directly in 1 ml of whole blood, without the need for blood culture.

  13. Untersuchungen zur Diagnostik und Epidemiologie von Staphylococcus aureus in Milchviehbetrieben in Brandenburg

    OpenAIRE

    Scheibe, Nicole

    2010-01-01

    The objective of this study was to analyse the epidemiological features of Staphylococcus (S.) aureus by using genotyping and antibiogram typing. Furthermore different methods for identification of S. aureus were performed to compare the methods’ ability to identify S. aureus from bovine milk. Milk samples were collected from six dairy herds with high prevalence of S. aureus in the federal state of Brandenburg, Germany. Of each herd, 32 cows in different stages of lactation and different a...

  14. In Vivo Activity of Ceftobiprole in Murine Skin Infections Due to Staphylococcus aureus and Pseudomonas aeruginosa▿

    OpenAIRE

    Fernandez, Jeffrey; Hilliard, Jamese J.; Abbanat, Darren; Zhang, Wenyan; Melton, John L.; Santoro, Colleen M.; Flamm, Robert K.; Bush, Karen

    2009-01-01

    Ceftobiprole, a broad-spectrum cephalosporin with activity against methicillin-resistant Staphylococcus aureus (MRSA) (P. Hebeisen et al., Antimicrob. Agents Chemother. 45:825-836, 2001), was evaluated in a subcutaneous skin infection model with Staphylococcus aureus Smith OC 4172 (methicillin-susceptible S. aureus [MSSA]), S. aureus OC 8525 (MRSA), Pseudomonas aeruginosa OC 4351 (having an inducible AmpC β-lactamase), and P. aeruginosa OC 4354 (overproducing AmpC β-lactamase). In the MSSA an...

  15. Investigation of the bactericidal effects of vancomycin and quinupristin/dalfopristin on Staphylococcus aureus isolates

    OpenAIRE

    HOŞGÖR-LİMONCU, Mine; ERMERTCAN, Şafak; COŞAR, Güner

    2004-01-01

    The present study aimed to determine the correlation between the bactericidal activity of vancomycin and quinupristin/dalfopristin (Q/D) on Staphylococcus aureus isolates and their minimal inhibition concentrations. The in-vitro susceptibilities of the 99 S. aureus isolates to vancomycin and Q/D were investigated by agar dilution. Thirty methicillin-resistant S. aureus (MRSA) and 30 methicillin-susceptible S. aureus (MSSA) vancomycin and Q/D susceptible isolates were involved in time-kill stu...

  16. Molecular Characterization and Antimicrobial Susceptibility of Nasal Staphylococcus aureus Isolates from a Chinese Medical College Campus

    OpenAIRE

    DU, JIMEI; Chen, Chun; Ding, Baixing; Tu, Jinjing; Qin, Zhiqiang; Parsons, Chris; Salgado, Cassandra; Cai, Qiangjun; SONG, Yulong; Bao, Qiyu; Zhang, Liming; Pan, Jingye; Wang, LiangXing; Yu, Fangyou

    2011-01-01

    Staphylococcus aureus colonization and infection occur more commonly among persons living or working in crowded conditions, but characterization of S. aureus colonization within medical communities in China is lacking. A total of 144 (15.4%, 144/935) S. aureus isolates, including 28 (3.0%, 28/935) MRSA isolates, were recovered from the nares of 935 healthy human volunteers residing on a Chinese medical college campus. All S. aureus isolates were susceptible to vancomycin, quinupristin/dalfopr...

  17. Daptomycin-nonsusceptible, vancomycin-intermediate, methicillin-resistant Staphylococcus aureus endocarditis

    OpenAIRE

    Ryan Yu; Dale, Suzanne E; Deborah Yamamura; Vida Stankus; Christine Lee

    2012-01-01

    Due to the emergence of Staphylococcus aureus with reduced vancomycin susceptibility, newer antibiotics, including daptomycin, have been used to treat methicillin-resistant S aureus infections. Daptomycin is a cyclic lipopeptide that is approved to treat S aureus bacteremia and right-sided endocarditis, and reports of S aureus with reduced susceptibility to daptomycin are infrequent. To our knowledge, the present report describes the first Canadian case of daptomycin-nonsusceptible, vancomyci...

  18. Human-associated Staphylococcus aureus strains within great ape populations in Central Africa (Gabon)

    OpenAIRE

    M. Nagel; Dischinger, J.; Türck, M.; Verrier, D.; Oedenkoven, M.; Ngoubangoye, B.; Le Flohic, G.; Drexler, J. F.; Bierbaum, G.; Gonzalez, Jean-Paul

    2013-01-01

    The risk of serious infections caused by Staphylococcus aureus is well-known. However, most studies regarding the distribution of (clinically relevant) S.aureus among humans and animals took place in the western hemisphere and only limited data are available from (Central) Africa. In this context, recent studies focused on S.aureus strains in humans and primates, but the question of whether humans and monkeys share related S.aureus strains or may interchange strains remained largely unsolved....

  19. Minimum inhibitory concentration of ciprofloxacin in combination with hexahydroquinoline derivatives against Staphylococcus aureus

    OpenAIRE

    F Amin Harati; Amini, M; AR Shahverdi; Pourmand MR; Yousefi, M

    2012-01-01

    Background: Staphylococcus aureus is the most common pathogen responsible for skin and soft tissue infections worldwide. Methicillin-resistant S. aureus is a major cause of both nosocomial and community acquired infections. The emergence of antimicrobial-resistant S. aureus is of global concern. Fluoroquinolone antimicrobials including ciprofloxacin, levofloxacin, and moxifloxacin are used to treat skin and soft tissue infections due to S. aureus. Emergence of ciprofloxacin resistance has inc...

  20. Methicillin-Resistant Staphylococcus aureus Colonization in Schoolteachers in Ontario

    Directory of Open Access Journals (Sweden)

    Beth A Hanselman

    2008-01-01

    Full Text Available A prospective study of methicillin-resistant Staphylococcus aureus (MRSA colonization was performed involving teachers at a science teachers’ conference in Toronto, Ontario. Nasal swabs and questionnaire data were collected from consenting individuals. MRSA colonization was identified in seven of 220 (3.2% participants. No colonized individuals reported recent contact with the health care system, antimicrobial therapy, residence with health care workers or previous MRSA infections. Methicillin-susceptible S aureus colonization was identified in 72 of 220 (33% individuals. The prevalence of MRSA colonization was higher than expected for a purportedly low-risk population.

  1. Genotyping of Staphylococcus aureus strains isolated from healthy persistent carriers.

    Science.gov (United States)

    Grzegorczyk, Agnieszka; Malm, Anna

    2014-07-01

    The paper presents results on the relatedness of Staphylococcus aureus strains colonizing the upper respiratory tract isolated from healthy persistent carriers. Genotyping was carried out using two methods--multiple-locus variable-number tandem-repeat fingerprinting (MLVF) and pulsed-field gel electrophoresis (PFGE). By comparison of the results obtained by both methods, good correlations between MLVF and PFGE genotyping of strains isolated from the asymptomatic carriers were observed. Further studies are needed to evaluate methods useful for genotyping of S. aureus strains circulating in the community. PMID:24488811

  2. Staphylococcus aureus sternal osteomyelitis: a rare cause of chest pain

    Directory of Open Access Journals (Sweden)

    Kaur M

    2015-10-01

    Full Text Available Chest pain is a common presenting symptom with a broad differential. Life-threatening cardiac and pulmonary etiologies of chest pain should be evaluated first. However, it is critical to perform a thorough assessment for other sources of chest pain in order to limit morbidity and mortality from less common causes. We present a rare case of a previously healthy 45 year old man who presented with focal, substernal, reproducible chest pain and Staphylococcus aureus bacteremia who was later found to have primary Staphylococcus aureus sternal osteomyelitis.

  3. Threat of multidrug resistant Staphylococcus aureus in Western Nepal

    DEFF Research Database (Denmark)

    Bhatta, Dharm R.; Cavaco, Lina; Nath, Gopal;

    2015-01-01

    ObjectiveTo determine the prevalence of methicillin resistant Staphylococcus aureus (MRSA) and antimicrobial susceptibility patterns of the isolates from Manipal Teaching Hospital, Pokhara, Nepal. MethodsThis study was conducted over a period of 11 months (September 2012–August 2013) at the Manipal...... using disc diffusion test by cefoxitin (30 μg) and oxacillin (1 μg) disc, further confirmation was done by detection of mecA gene using PCR. ResultsOut of 400 Staphylococcus aureus strains, 139 (34.75%) were found to be MRSA. Among the MRSA isolates, 74 (53.2%) were from inpatient departments, 58 (41...

  4. Capturing of staphylococcus aureus onto an interface containing graft chains

    International Nuclear Information System (INIS)

    A microbial-cell-capturing material was prepared by radiation-induced grafting of glycidyl methacrylate onto a polyethylene-based fiber before the introduction of diethylamine. The prepared fiber was tested against a Staphylococcus aureus and Escherichia coli solution. The results showed that the grafted-type fiber had a capturing rate constant 1000-fold higher than the commercial crosslinked-type bead for S. aureus and that an activation energy of 39 kJ/mol was obtained for the microbial-cell-capturing action. (author)

  5. Methicillin-resistant Staphylococcus aureus colonization in schoolteachers in Ontario.

    Science.gov (United States)

    Hanselman, Beth A; Kruth, Steven A; Rousseau, Joyce; Weese, J Scott

    2008-11-01

    A prospective study of methicillin-resistant Staphylococcus aureus (MRSA) colonization was performed involving teachers at a science teachers' conference in Toronto, Ontario. Nasal swabs and questionnaire data were collected from consenting individuals. MRSA colonization was identified in seven of 220 (3.2%) participants. No colonized individuals reported recent contact with the health care system, antimicrobial therapy, residence with health care workers or previous MRSA infections. Methicillin-susceptible S aureus colonization was identified in 72 of 220 (33%) individuals. The prevalence of MRSA colonization was higher than expected for a purportedly low-risk population. PMID:19436569

  6. Response of Staphylococcus Aureus to a Spaceflight Analogue

    Science.gov (United States)

    Castro, S. L.; Ott, C. M.

    2010-01-01

    The decreased gravity of the spaceflight environment creates quiescent, low fluid shear conditions. This environment can impart considerable effects on the physiology of microorganisms as well as their interactions with potential hosts. Using the rotating wall vessel (RWV), as a spaceflight analogue, the consequence of low fluid shear culture on microbial pathogenesis has provided a better understanding of the risks to the astronaut crew from infectious microorganisms. While the outcome of low fluid shear culture has been investigated for several bacterial pathogens, little has been done to understand how this environmental factor affects Staphylococcus aureus. S. aureus is an opportunistic human pathogen which presents a high level of infection risk to the crew, as it has been isolated from both the space shuttle and International Space Station. Given that approximately forty percent of the population are carriers of the bacteria, eradication of this organism from in flight environments is impractical. These reasons have lead to us to assess the response of S. aureus to a reduced fluid shear environment. Culture in the RWV demonstrated that S. aureus grown under the low-shear condition had lower cell concentrations after 10 hours when compared to the control culture. Furthermore, the low-shear cultured bacteria displayed a reduction in carotenoid production, pigments responsible for their yellow/gold coloration. When exposed to various environmental stressors, post low-shear culture, a decrease in the ability to survive oxidative assault was observed compared to control cultures. The low fluid shear environment also resulted in a decrease in hemolysin secretion, a staphylococcal toxin responsible for red blood cell lysis. When challenged by the immune components present in human whole blood, low-shear cultured S. aureus demonstrated significantly reduced survival rates as compared to the control culture. Assays to determine the duration of these alterations

  7. Mechanism of bacteriophage conversion of lipase activity in Staphylococcus aureus.

    OpenAIRE

    Lee, C Y; Iandolo, J J

    1985-01-01

    Staphylococcus aureus PS54 harbors two temperate bacteriophages and manifests no lipase activity on egg yolk agar. Curing of one of the resident prophages (L54a) restores lipase activity. To study the mechanism of bacteriophage conversion, the prophage was cured, and the gene encoding lipase activity was cloned into pBR322 in Escherichia coli on a 2.9-kilobase DNA fragment of the chromosome. The fragment was subcloned into a shuttle vector and subsequently transformed into S. aureus and Bacil...

  8. THE LETTER OF GUARANTEE FROM THE PERSPECTIVE OF THE NEW CIVIL CODE

    Directory of Open Access Journals (Sweden)

    LUMINITA TULEASCA

    2011-04-01

    Full Text Available The letter of guarantee is frequently used in the domestic and international commercial activity, first of all, for the safety offered for securing the contractual obligations without blocking the pecuniary funds and, second of all, due to the existing uniform international regulations that correspond to the needs supporting the occurrence of these types of autonomous securities.In Romania, the lack of legal policing of the letter of guarantee has created practical difficulties and offered judges the possibility to "make the law" in the litigations generated by the enforcement or by the suspension of enforcement of these contractual securities.This work analysis the regulation of the letter of guarantee provided in the New Civil Code from the perspective of its harmonization with the Uniform Rules of the International Chamber of Commerce in Paris and with the Draft Common Frame of Reference in the matter of the European private law.

  9. The structure and economic significance of government guarantees in Croatia and the European Union

    Directory of Open Access Journals (Sweden)

    Marko Primorac

    2016-03-01

    Full Text Available In the aftermath of the financial crisis, when countries are facing difficulties in raising the amounts of revenue needed to cover the expenditure side of the budget, fiscal risks can pose a significant threat to the sustainability of public finance. This became particularly evident in the case of public enterprises and their liabilities, which often increased public debt because of difficulties in meeting their financial obligations. The aim of this paper is to evaluate fiscal risks from government guarantees in Croatia and the European Union in general. Moreover, the paper aims to analyse the dynamics of the value and structure of government guarantees in Croatia in the period from 2009 to first half of 2015. Particular emphasis is placed on the impact of government guarantees on direct public debt in the context of methodological changes in the registration of public debt.

  10. Statistical expedited forwarding service for per-flow QoS guarantees

    Science.gov (United States)

    Sun, Yeali; Chen, Wen-Jen; Chen, Meng-Chang

    2001-07-01

    In this paper we propose a new scheme Statistical Expedited Forwarding to support a new class of service that guarantees bounded delay and bounded packet loss performance. The goal of the proposed scheme is to provide the Integrated Services type of performance guarantees, but without maintaining per-flow state in the network core. The target applications are both delay-sensitive and loss-sensitive like IP telephony, video applications and emerging on-line electronic business-related applications. In order to guarantee delay and loss bounds, the effective resource (consisting of bandwidth and buffer) is assigned to each class according to the given QoS requirement. In addition, we further study the fairness among flows in a class. The experiments on several voice and video applications show the strength of the proposed scheme.

  11. Performance Enhancement and Bandwidth Guarantee in IEEE 802.11 Wireless LANs

    Institute of Scientific and Technical Information of China (English)

    Yong Peng; Shi-Duan Cheng

    2004-01-01

    This paper first revisits the previously proposed NSAD (New Self-Adapt DCF) mechanism. Some modifications are presented to further enhance the performance of NSAD in the error-prone environment. Then a new MAC mechanism is proposed that can realize bandwidth guarantee by assigning different self-adapt parameters to users at different priority levels. The bandwidth guarantee property of this new mechanism is analyzed and the high priority users are found to have bandwidth guaranteed even in heavy contention condition, which is proved true not only by theoretical analysis but also by simulation results. At the same time the new scheme keeps the self-adapt character of NSAD, sothe overall system utilization is kept very high in heavy contention condition compared with the previously studied DCF-based QoS mechanisms.

  12. Guaranteed cost control with constructing switching law of uncertain discrete-time switched systems

    Institute of Scientific and Technical Information of China (English)

    Zhang Ying; Duan Guangren

    2007-01-01

    A guaranteed cost control problem for a class of linear discrete-time switched systems with normbounded uncertainties is considered in this article. The purpose is to construct a switching rule and design a state feedback control law, such that, the closed-loop system is asymptotically stable and the closed-loop cost function value is not more than a specified upper bound for all admissible uncertainties under the constructed switching rule.A sufficient condition for the existence of guaranteed cost controllers and switching rules is derived based on the Lyapunov theory together with the linear matrix inequality (LMI) approach. Furthermore, a convex optimization problem with LMI constraints is formulated to select the suboptimal guaranteed cost controller. A numerical example demonstrates the validity of the proposed design approach.

  13. Guaranteed cost control with pole constraints for uncertain discrete-time switched systems

    Institute of Scientific and Technical Information of China (English)

    Ying ZHANG; Guangren DUAN; Liyan CHEN

    2009-01-01

    For a class of discrete-time switched systems with norm-bounded uncertainties and a quadratic cost index, the problem of designing a guaranteed cost state feedback controller with pole constraints is considered. A sufficient condition on the existence of robust guaranteed controllers is derived by a quadratic Lyapunov function approach together with linear matrix inequality (LMI)technique. Based on a constructed switching law, the closed-loop system is quadratic D-stable and the closedloop cost function value is not more than a specified upper bound. Furthermore, the design of suboptimal guaranteed cost controllers is turned into a convex optimization problem with linear matrix inequalities constraints. A numerical example demonstrates the effect of the proposed design approach.

  14. Non-fragile guaranteed cost control for uncertain neutral large-scale interconnected systems

    Institute of Scientific and Technical Information of China (English)

    Dan Zhao; Qingling Zhang; Heli Hu; Chunyuan Zhao

    2010-01-01

    This paper focuses on the problem of non-fragile decentralized guaranteed cost control for uncertain neutral large-scale interconnected systems with time-varying delays in state,control input and interconnections.A novel scheme,viewing the interconnections with time-varying delays as effective information but not disturbances,is developed.Based on Lyapunov stability theory,using various techniques of decomposing and magnifying matrices,a design method of the non-fragile decentralized guaranteed cost controller for unperturbed neutral large-scale interconnected systems is proposed and the guaranteed cost is presented.The further results are derived for the uncertain case from the criterion of unperturbed neutral large-scale interconnected systems.Finally,an illustrative example shows that the results are significantly better than the existing results in the literatures.

  15. Frequency of methicillin-resistant Staphylococcus aureus nasal colonization among patients suffering from methicillin resistant Staphylococcus aureus bacteraemia

    Science.gov (United States)

    Aslam, Nadia; Izhar, Mateen; Mehdi, Naima

    2013-01-01

    Objective: To determine rate of nasal colonization in Patients suffering from bacteraemia caused by methicillin resistant Staphylococcus aureus. Methods: This descriptive cross sectional study was carried out in a tertiary ca re, University Teaching Hospital (Shaikh Zayed Hospital, Lahore) from October 2010 to August 2011. Nasal swabs were taken from patients suffering from MRSA bacteraemia and were plated on mannitol salt agar plates to isolate Staphylococcus aureus (S. aureus) which were then tested for oxacillin susceptibility. Results: Nasal colonization was present in 52.5% of patients suffering from MRSA bacteraemia. Conclusion: Nasal colonization rates with MRSA were high among patients suffering from MRSA bacteraemia especially in those undergoing dialysis or surgical procedures. Therefore, screening and nasal decolonization should be practiced in hospitals. PMID:24550968

  16. Phenotypic and genotypic antimicrobial resistance traits of foodborne Staphylococcus aureus isolates from Shanghai

    Science.gov (United States)

    Staphylococcus aureus is a recognized pathogen in humans, which causes nosocomial infections and food poisoning. The transmission of antibiotic resistant S. aureus (ARSA), especially methicillin-resistant S. aureus (MRSA), between food products and humans has become a serious problem. Hence, it is n...

  17. Antimicrobial Susceptibility Pattern and Biochemical Characteristics of Staphylococcus aureus: Impact of Bio field Treatment

    OpenAIRE

    Mahendra Kumar Trivedi

    2015-01-01

    Study background: Staphylococci are widespread in nature, mainly found on the skin and mucous membranes. Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stable exotoxins. The current study was attempted to investigate the effect of biofield treatment on antimicrobial susceptibility pattern and biochemical characteristics of S. aureus (ATCC 25923).

  18. Nosocomial Infections and Drug Susceptibility Patterns in Methicillin Sensitive and Methicillin Resistant Staphylococcus aureus

    OpenAIRE

    Sharma, Nitish Kumar; Garg, Raina; Baliga, Shrikala; Bhat K., Gopalkrishna

    2013-01-01

    Aim: Staphylococcus aureus is one of the leading causes of nosocomial infections and is known for its ability to develop resistance to antibiotics. The drug susceptibility pattern of Methicillin Sensitive S. aureus (MSSA) and Methicillin Resistant S. aureus (MRSA) may vary.

  19. Staphylococcus aureus Strains That are Hypersusceptible to Resistance Gene Transfer from Enterococci▿

    OpenAIRE

    Sung, Julia M.-L.; Lindsay, Jodi A

    2007-01-01

    We identified naturally occurring Staphylococcus aureus mutants of the restriction modification pathway SauI, including bovine lineage ST151. In a model of vancomycin resistance transfer from Enterococcus faecalis, ST151 isolates are 500 times more susceptible than human S. aureus isolates. The eradication of “hyperrecipient” strains may reduce the evolution of vancomycin-resistant S. aureus.

  20. The population structure of Staphylococcus Aureus among general practice patients from The Netherlands.

    NARCIS (Netherlands)

    Donker, G.A.; Deurenberg, R.H.; Driessen, C.; Sebastian, S.; Nijs, S.; Stobberingh, E.E.

    2009-01-01

    To investigate the prevalence, the antibiotic resistance pattern and the population structure of Staphylococcus aureus, S. aureus isolates from the anterior nostrils of patients of general practitioners (GPs) were analysed. Insight into the S. aureus population structure is essential, as nasal carri

  1. Genome Sequences of Four Staphylococcus aureus Strains Isolated from Bovine Mastitis

    OpenAIRE

    Kant, Ravi; Taponen, Suvi; Koort, Joanna; Paulin, Lars; Åvall-Jääskeläinen, Silja; Palva, Airi

    2015-01-01

    Staphylococcus aureus is a major causative agent of mastitis in dairy cows. The pathogenicity of S. aureus may vary; it is able to cause severe clinical mastitis, but most often it is associated with chronic subclinical mastitis. Here, we present the genome assemblies of four S. aureus strains from bovine mastitis.

  2. Genome Sequences of Four Staphylococcus aureus Strains Isolated from Bovine Mastitis.

    Science.gov (United States)

    Kant, Ravi; Taponen, Suvi; Koort, Joanna; Paulin, Lars; Åvall-Jääskeläinen, Silja; Palva, Airi

    2015-01-01

    Staphylococcus aureus is a major causative agent of mastitis in dairy cows. The pathogenicity of S. aureus may vary; it is able to cause severe clinical mastitis, but most often it is associated with chronic subclinical mastitis. Here, we present the genome assemblies of four S. aureus strains from bovine mastitis. PMID:25908141

  3. Legal Guarantees and Inconsistency of State Recognition to the Right of Religion/Belief in Indonesia

    OpenAIRE

    Kadarudin

    2015-01-01

    The right to freedom of religion/belief has been guaranteed by law in Indonesia, but there are still going on some violations of the right to freedom of religion/belief in the various regions. The purpose of this study was to determine the form of the legal guarantee of the right to freedom of religion/belief in Indonesia, shape inconsistencies state recognition of the right to religion/belief in Indonesia, and efforts to improve the rights of religion/belief in Indonesia. This paper uses a n...

  4. Design of Robust Guaranteed Cost PID Controller for Networked Control Systems

    Science.gov (United States)

    Nguyen, Quang Thuan; Veselý, Vojtech

    2010-03-01

    The paper addresses the problem of an output feedback guaranteed cost controller design for Networked Control Systems (NCSs) with time-delay and polytopic uncertainties. By constructing a new parameter-dependent Lyapunov functional and applying the free-weighting matrices technique, the parameter-dependent, delay-dependent design method will be obtained to synthesize PID controllers achieving a guaranteed cost such that the NCSs can be stabilized for all admissible uncertainties and time-delays. Finally, numerical examples are given to illustrate the effectiveness of the proposed method.

  5. Control the guaranteed search of moving objects at minimum expense of light energy

    Directory of Open Access Journals (Sweden)

    Avetisyan V.V.

    2008-03-01

    Full Text Available An algorithm of control manipulator gripper movement in given light conditions with optimal, minimum expense of light energy has been developed, provides the location of the searched object within the planned time frame. It has been found and justified through digital calculations that optimal processes of guaranteed search are possible which require same volume of light energy expense, but, however, in one case the optimal guaranteed search is implemented quickly and needs a great light source, while in the other case the requirements are opposite.

  6. State Feedback Guaranteed Cost Repetitive Control for Uncertain Discrete-Time Systems

    OpenAIRE

    Qingping Wu; Yechun Lin; Wentao Chen

    2011-01-01

    This paper considers the problem of guaranteed cost repetitive control for uncertain discrete-time systems. The uncertainty in the system is assumed to be norm-bounded and time-varying. The objective is to develop a novel design method so that the closed-loop repetitive control system is quadratically stable and a certain bound of performance index is guaranteed for all admissible uncertainties. The state feedback control technique is used in the paper. While for the case that the states are ...

  7. Administrative Circular No. 13 (Rev. 4) - Guarantees for representatives of the personnel

    CERN Multimedia

    Department Head Office - HR Department

    2016-01-01

    Administrative Circular No. 13 (Rev. 4) entitled "Guarantees for representatives of the personnel", approved by the Director-General following discussion in the Standing Concertation Committee meeting on 22 March 2016, will be available on 1st September 2016 via the following link: https://cds.cern.ch/record/2208527.   This revised circular cancels and replaces Administrative Circular No. 13 (Rev. 3) also entitled "Guarantees for representatives of the personnel" of January 2014. This document contains a single change to reflect the terminology under the new career structure: the term "career path" is replaced by "grade". This circular will enter into force on 1st September 2016.

  8. Time-optimal guaranteed search of mobile object in the plane

    Directory of Open Access Journals (Sweden)

    Avetisyan V.V.

    2015-03-01

    Full Text Available A problem of time-optimal guaranteed search of moving object with controllable speed in plane is observed, where in the initial moment only the circular of the sought object is known. The sought object is a object with controllable speed. The discovery, i.e. the identification of the precise coordinates of the object, is implemented through the moving information basis of the cone, the apex of which is connected to the current coordinates of the sough object. It is required to locate the object as quickly as possible. A control algorithm is proposed, in which r a guaranteed search of the sought object in minimal time is completed.

  9. Guaranteed Cost Control for Uncertain Nonlinear Time-Delay Neutral Systems Based on T-S Fuzzy Model

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The problem of guaranteed cost fuzzy controller is studied for a class of nonlinear time-delay neutral systems with norm-bounded uncertainty based on T-S model. The sufficient conditions are first derived for the existence of guaranteed cost fuzzy controllers. These sufficient conditions are equivalent to a kind of linear matrix inequalities. Furthermore, a convex optimization problem with LMI constraints is formulated to design the optimal guaranteed cost controller.

  10. Alternative Labeling Programs and Purchasing Behavior toward Organic Foods: The Case of the Participatory Guarantee Systems in Brazil

    OpenAIRE

    Giovanna Sacchi; Vincenzina Caputo; Nayga, Rodolfo M.

    2015-01-01

    Regulatory standards and certification models are essential tools guaranteeing the authenticity of organic products. In particular, third-party certification is useful to consumers since it provides guarantees regarding production processes and food quality. In an attempt to cope with the costs and bureaucratic procedures related to the adoption of such certification, groups of small producers have begun to rely upon alternative quality assurance systems such as Participatory Guarantee System...

  11. Optimal Iterative Learning Fault-Tolerant Guaranteed Cost Control for Batch Processes in the 2D-FM Model

    OpenAIRE

    Limin Wang; Weiwei Dong

    2012-01-01

    This paper develops the optimal fault-tolerant guaranteed cost control scheme for a batch process with actuator failures. Based on an equivalent two-dimensional Fornasini-Marchsini (2D-FM) model description of a batch process, the relevant concepts of the fault-tolerant guaranteed cost control are introduced. The robust iterative learning reliable guaranteed cost controller (ILRGCC), which includes a robust extended feedback control for ensuring the performances over time and an iterative lea...

  12. Guaranteed capacity of hydro power plants in Germany and Austria; Gesicherte Leistung der Wasserkraft in Deutschland und Oesterreich

    Energy Technology Data Exchange (ETDEWEB)

    Pfleger, Markus [VERBUND Hydro Power GmbH, Wien (Austria); Ernst, Hans-Peter; Engels, Klaus [E.ON Kraftwerke GmbH, Sparte Wasserkraft, Landshut (Germany); Metzka, Rudolf [Technische Hochschule Deggendorf (Germany)

    2015-11-01

    Balancing fluctuations caused by renewable energy play an important role in the current transition of energy supply systems. Hydro power plants provide both: guaranteed capacity as well as renewability. In Germany and Austria definitions of how to quantify the guaranteed capacity of hydro power plants are well established to calculate the guaranteed capacity for an individual power plant. The definitions are challenged against real examples.

  13. Staphylococcus aureus with reduced susceptibility to vancomycin in healthcare settings.

    Science.gov (United States)

    Spagnolo, A M; Orlando, P; Panatto, D; Amicizia, D; Perdelli, F; Cristina, M L

    2014-12-01

    Glycopeptide resistance in Staphylococcus aureus is a source of great concern because, especially in hospitals, this class of antibiotics, particularly vancomycin, is one of the main resources for combating infections caused by methicillin-resistant Staphylococcus aureus strains (MRSA). Reduced susceptibility to vancomycin (VISA) was first described in 1996 in Japan; since then, a phenotype with heterogeneous resistance to vancomycin (h-VISA) has emerged. H-VISA isolates are characterised by the presence of a resistant subpopulation, typically at a rate of 1 in 10(5) organisms, which constitutes the intermediate stage betweenfully vancomycin-susceptible S. aureus (VSSA) and VISA isolates. As VISA phenotypes are almost uniformly cross-resistant to teicoplanin, they are also called Glycopeptides-intermediate Staphylococcus aureus strains (GISA) and, in the case of heterogeneous resistance to glycopeptides, h-GISA. The overall prevalence of h-VISA is low, accounting for approximately 1.3% of all MRSA isolates tested. Mortality due to h-GISA infections is very high (about 70%), especially among patients hospitalised in high-risk departments, such as intensive care units (ICU). Given the great clinical relevance of strains that are heteroresistant to glycopeptides and the possible negative impact on treatment choices, it is important to draw up and implement infection control practices, including surveillance, the appropriate use of isolation precautions, staff training, hand hygiene, environmental cleansing and good antibiotic stewardship. PMID:26137787

  14. Vancomycin-resistant Staphylococcus aureus: no apocalypse now.

    Science.gov (United States)

    Goldstein, F W; Kitzis, M D

    2003-08-01

    The number of reports concerning vancomycin-resistant Staphylococcus aureus is much higher than the number of true resistant strains or unexpected clinical failures. Many confounding factors, including inadequate serum levels, severely ill patients, foreign devices or undrained abscesses, are more likely to be responsible for the clinical failures than resistance to vancomycin. PMID:14616695

  15. Review on Panton Valentine leukocidin toxin carriage among Staphylococcus aureus.

    Science.gov (United States)

    Shrestha, B

    2013-09-01

    Panton Valentine leukocidin is a toxin making pores in the polymorphonuclear cells which is a virulence factor of some strains of Staphylococcus aureus. Initially it was produced by methicillin susceptible Staphylococcus aureus only. Later with the acquisition of mecA gene has lead it to be PVL positive methicillin resistant Staphylococcus aureus. Since MRSA are resistant to many antibiotics and further they produce a toxin the infections by PVL positive MRSA has become a challenge. PVL positive MRSA a virulent strain of drug resistant superbug MRSA that has spread around the world, has claimed many lives in UK, Europe, USA and Australia. Some strains of superbug attack the healthy young people and kill within 24 hrs. PVL positive Staphylococcus aureus has been reported to be associated with skin and soft tissue infections however they also cause invasive infections and necrotizing pneumonia. These microorganisms known to be community associated have spread to hospitals. Hospital acquired infection by such microorganisms lead to an increase in mortality hence should be controlled before they become prevalent in hospitals. PMID:24908537

  16. An Interdisciplinary Experiment: Azo-Dye Metabolism by "Staphylococcus Aureus"

    Science.gov (United States)

    Brocklesby, Kayleigh; Smith, Robert; Sharp, Duncan

    2012-01-01

    An interdisciplinary and engaging practical is detailed which offers great versatility in the study of a qualitative and quantitative metabolism of azo-dyes by "Staphylococcus aureus". This practical has broad scope for adaptation in the number and depth of variables to allow a focused practical experiment or small research project. Azo-dyes are…

  17. Increased risk of arterial thromboembolic events after Staphylococcus aureus bacteremia

    DEFF Research Database (Denmark)

    Mejer, N; Gotland, N; Uhre, M L;

    2015-01-01

    OBJECTIVES: An association between infection and arterial thromboembolic events (ATE) has been suggested. Here we examined the risk of myocardial infarction (MI), stroke and other ATE after Staphylococcus aureus bacteremia (SAB). METHODS: Danish register-based nation-wide observational cohort study...

  18. Pyrazole Based Inhibitors against Enzymes of Staphylococcus aureus

    DEFF Research Database (Denmark)

    Jagadeesan, G.; Vijayakuma, Vinodhkumar; Palayam, Malathy;

    2015-01-01

    agents. The current study focuses on molecular docking and dynamics studies of pyrazole derivatives against Nucleosidase and DNA gyrase B of Staphylococcus aureus. Molecular docking and dynamics studies reveal that some of these derivatives show better binding abilities than some of the current drugs...

  19. Killing of Staphylococcus aureus by C-8-Methoxy Fluoroquinolones

    OpenAIRE

    Zhao, Xilin; Wang, Jian-Ying; Xu, Chen; Dong, Yuzhi; Zhou, Jianfeng; Domagala, John; Drlica, Karl

    1998-01-01

    C-8-methoxy fluoroquinolones were more lethal than C-8-bromine, C-8-ethoxy, and C-8-H derivatives for Staphylococcus aureus, especially when topoisomerase IV was resistant. The methoxy group also increased lethality against wild-type cells when protein synthesis was inhibited. These properties encourage refinement of C-8-methoxy fluoroquinolones to kill staphylococci.

  20. Simple method for correct enumeration of Staphylococcus aureus.

    Science.gov (United States)

    Haaber, J; Cohn, M T; Petersen, A; Ingmer, H

    2016-06-01

    Optical density (OD) measurement is applied universally to estimate cell numbers of microorganisms growing in liquid cultures. It is a fast and reliable method but is based on the assumption that the bacteria grow as single cells of equal size and that the cells are dispersed evenly in the liquid culture. When grown in such liquid cultures, the human pathogen Staphylococcus aureus is characterized by its aggregation of single cells into clusters of variable size. Here, we show that aggregation during growth in the laboratory standard medium tryptic soy broth (TSB) is common among clinical and laboratory S. aureus isolates and that aggregation may introduce significant bias when applying standard enumeration methods on S. aureus growing in laboratory batch cultures. We provide a simple and efficient sonication procedure, which can be applied prior to optical density measurements to give an accurate estimate of cellular numbers in liquid cultures of S. aureus regardless of the aggregation level of the given strain. We further show that the sonication procedure is applicable for accurate determination of cell numbers using agar plate counting of aggregating strains. PMID:27080188

  1. Staphylococcus aureus redirects central metabolism to increase iron availability.

    Directory of Open Access Journals (Sweden)

    David B Friedman

    2006-08-01

    Full Text Available Staphylococcus aureus pathogenesis is significantly influenced by the iron status of the host. However, the regulatory impact of host iron sources on S. aureus gene expression remains unknown. In this study, we combine multivariable difference gel electrophoresis and mass spectrometry with multivariate statistical analyses to systematically cluster cellular protein response across distinct iron-exposure conditions. Quadruplicate samples were simultaneously analyzed for alterations in protein abundance and/or post-translational modification state in response to environmental (iron chelation, hemin treatment or genetic (Deltafur alterations in bacterial iron exposure. We identified 120 proteins representing several coordinated biochemical pathways that are affected by changes in iron-exposure status. Highlighted in these experiments is the identification of the heme-regulated transport system (HrtAB, a novel transport system which plays a critical role in staphylococcal heme metabolism. Further, we show that regulated overproduction of acidic end-products brought on by iron starvation decreases local pH resulting in the release of iron from the host iron-sequestering protein transferrin. These findings reveal novel strategies used by S. aureus to acquire scarce nutrients in the hostile host environment and begin to define the iron and heme-dependent regulons of S. aureus.

  2. Complete Genome Sequence of Staphylococcus aureus Siphovirus Phage JS01

    OpenAIRE

    Jia, Hongying; Bai, Qinqin; Yang, Yongchun; Yao, Huochun

    2013-01-01

    Staphylococcus aureus is the most prevalent and economically significant pathogen causing bovine mastitis. We isolated and characterized one staphylophage from the milk of mastitis-affected cattle and sequenced its genome. Transmission electron microscopy (TEM) observation shows that it belongs to the family Siphovirus. We announce here its complete genome sequence and report major findings from the genomic analysis.

  3. Natural Population Dynamics and Carriage of Staphylococcus aureus

    NARCIS (Netherlands)

    D.C. Melles (Damian)

    2008-01-01

    textabstractStaphylococcus aureus is a major human pathogen capable of causing a wide range of infections, from relatively mild skin infections such as folliculitis and furunculosis to life-threatening conditions, including sepsis, deep abscesses, pneumonia, osteomyelitis, and infective endocarditis

  4. THE STUDY OF RESISTENCE OF STAPHYLOCOCCUS AUREUS STRAINS TO ANTIMICROBIALS

    Directory of Open Access Journals (Sweden)

    Nazarchuk GG

    2012-12-01

    Full Text Available In the research work the results of the study of resistance forming to antibiotics, antiseptics and decametoxine composition with modified polysaccharides in S.aureus strains are presented. The development of resistance to penicillins, cephalosporins, glycopeptides, macrolides is shown. Slow forming of resistance to decasan and decametoxine composition with carboxymethylamylum, oxyethylcellulose was determined.

  5. Human Staphylococcus aureus lineages among Zoological Park residents in Greece

    Directory of Open Access Journals (Sweden)

    E. Drougka

    2015-10-01

    Full Text Available Staphylococcus aureus is a part of the microbiota flora in many animal species. The clonal spread of S. aureus among animals and personnel in a Zoological Park was investigated. Samples were collected from colonized and infected sites among 32 mammals, 11 birds and eight humans. The genes mecA, mecC, lukF/lukS-PV (encoding Panton-Valentine leukocidin, PVL and tst (toxic shock syndrome toxin-1 were investigated by PCR. Clones were defined by Multilocus Sequence Typing (MLST, spa type and Pulsed-Field Gel Electrophoresis (PFGE. Seven S. aureus isolates were recovered from four animals and one from an employee. All were mecA, mecC and tst–negative, whereas, one carried the PVL genes and was isolated from an infected Squirrel monkey. Clonal analysis revealed the occurrence of seven STs, eight PFGE and five spa types including ones of human origin. Even though a variety of genotypes were identified among S. aureus strains colonizing zoo park residents, our results indicate that colonization with human lineages has indeed occurred.

  6. Genetic Diversity of Staphylococcus aureus in Buruli Ulcer

    NARCIS (Netherlands)

    Amissah, Nana Ama; Glasner, Corinna; Ablordey, Anthony; Tetteh, Caitlin S.; Kotey, Nana Konama; Prah, Isaac; van der Werf, Tjip; Rossen, John W.; van Dijl, Jan Maarten; Stienstra, Ymkje

    2015-01-01

    Background Buruli ulcer (BU) is a necrotizing skin disease caused by Mycobacterium ulcerans. Previous studies have shown that wounds of BU patients are colonized with M. ulcerans and several other microorganisms, including Staphylococcus aureus, which may interfere with wound healing. The present st

  7. New insights into molecular typing methods for Staphylococcus aureus

    NARCIS (Netherlands)

    Ikawaty, R.

    2009-01-01

    Staphylococcus aureus (SA) remains a significant problem causing infections in both hospital and community settings. Methicillin-resistant SA (MRSA) continues to evolve and pose a great challenge through outbreaks and pandemic spread. Humans are no longer the only and the most important reservoir of

  8. Vancomycin-Resistant Staphylococcus aureus, Michigan, USA, 2007

    OpenAIRE

    Finks, Jennie; Wells, Eden; Dyke, Teri Lee; Husain, Nasir; Plizga, Linda; Heddurshetti, Renuka; Wilkins, Melinda; Rudrik, James; Hageman, Jeffrey; Patel, Jean; Miller, Corinne

    2009-01-01

    Vancomycin-resistant Staphylococcus aureus (VRSA) infections, which are always methicillin-resistant, are a rare but serious public health concern. We examined 2 cases in Michigan in 2007. Both patients had underlying illnesses. Isolates were vanA-positive. VRSA was neither transmitted to or from another known VRSA patient nor transmitted from patients to identified contacts.

  9. Virulence Factors and Antibiotic Susceptibility of Staphylococcus aureus Isolates in Ready-to-Eat Foods: Detection of S. aureus Contamination and a High Prevalence of Virulence Genes

    OpenAIRE

    Suat Moi Puah; Kek Heng Chua; Jin Ai Mary Anne Tan

    2016-01-01

    Staphylococcus aureus is one of the leading causes of food poisoning. Its pathogenicity results from the possession of virulence genes that produce different toxins which result in self-limiting to severe illness often requiring hospitalization. In this study of 200 sushi and sashimi samples, S. aureus contamination was confirmed in 26% of the food samples. The S. aureus isolates were further characterized for virulence genes and antibiotic susceptibility. A high incidence of virulence genes ...

  10. Expression and inducibility in Staphylococcus aureus of the mecA gene, which encodes a methicillin-resistant S. aureus-specific penicillin-binding protein.

    OpenAIRE

    Ubukata, K; Nonoguchi, R; Matsuhashi, M; Konno, M

    1989-01-01

    A beta-lactam-sensitive strain of Staphylococcus aureus could be converted to methicillin resistance by the introduction of a plasmid carrying the 4.3-kilobase HindIII chromosomal DNA fragment which encoded the mecA gene from a methicillin-resistant S. aureus. Transformant cells produced methicillin-resistant S. aureus-specific penicillin-binding protein constitutively, and additional insertion of an inducible penicillinase plasmid caused production of the pencillin-binding protein to become ...

  11. Complex network perspective on structure and function of Staphylococcus aureus metabolic network

    Indian Academy of Sciences (India)

    L Ying; D W Ding

    2013-02-01

    With remarkable advances in reconstruction of genome-scale metabolic networks, uncovering complex network structure and function from these networks is becoming one of the most important topics in system biology. This work aims at studying the structure and function of Staphylococcus aureus (S. aureus) metabolic network by complex network methods. We first generated a metabolite graph from the recently reconstructed high-quality S. aureus metabolic network model. Then, based on `bow tie' structure character, we explain and discuss the global structure of S. aureus metabolic network. The functional significance, global structural properties, modularity and centrality analysis of giant strong component in S. aureus metabolic networks are studied.

  12. Fair Valuation of Guaranteed Contracts : The Interaction Between Assets and Liabilities

    NARCIS (Netherlands)

    Charlier, E.; Kleynen, R.H.M.A.

    2005-01-01

    In this paper we study the investment behavior of an insurance company consisting of policy and equity holders and issuing fair valued guaranteed contracts.Building up on the work of Briys and De Varenne (1997) and De Munnik and Schotman (1994) an intertemporal stochastic asset model and an interest

  13. 76 FR 30904 - Rural Utilities Service Telecommunications Loan and Loan Guarantee Program

    Science.gov (United States)

    2011-05-27

    ... Rural Utilities Service Rural Utilities Service Telecommunications Loan and Loan Guarantee Program... telecommunications systems, equipment and services. The List of Materials historically has been a very useful tool in... to advancing state-of-the-art telecommunications technologies, consistent with our commitment to...

  14. 7 CFR 1717.655 - Exclusion of certain investments, loans, and guarantees.

    Science.gov (United States)

    2010-01-01

    ... that serves primarily consumers located in rural areas as defined in 7 CFR 1710.2, and guarantee debt... waste disposal systems; (C) Telecommunication and other electronic communication systems; and (D... § 1717.657(c), or has approved a related transaction (e.g., a lien accommodation)....

  15. 77 FR 54877 - Guaranteed Rural Rental Housing Low Loan-to-Cost Ratio

    Science.gov (United States)

    2012-09-06

    ... U.S.C. 1490p-2) and operates under 7 CFR part 3565. The low loan-to- cost ratio is defined at 50... Rural Housing Service Guaranteed Rural Rental Housing Low Loan-to-Cost Ratio AGENCY: Rural Housing Service, USDA. ACTION: Notice. SUMMARY: The Rural Housing Service announces the low loan-to-cost...

  16. How Does Strategic Choice Affect Business Results? A Case Study of Mutual Guarantee Societies

    OpenAIRE

    Pedro Núñez-Cacho Utrilla; Félix Angel Grande Torraleja; Agustín Muñoz Vázquez; Manuel Aranda Ogáyar

    2012-01-01

    We analyzed the relationship between various strategies and the results for certain strategic variables for MutualGuarantee Societies (MGSs). After performing a factor analysis, three types of strategies wereidentified. Subsequently, cluster analysis allowed us to group the MGSs into four clusters. The informationobtained from this analysis suggests that MGS results are conditioned by their strategies.

  17. Financial Audit: Guaranteed Student Loan Program's Internal Controls and Structure Need Improvement. Report to the Congress.

    Science.gov (United States)

    Wurtz, Donald R.; And Others

    An evaluative study was done of the Department of Education's system of internal accounting controls over the Federal Family Education Loan Program, known as the guaranteed student loan program. The study evaluated internal control systems, the structure of the program with respect to the role of guaranty agencies, and the Department's ability to…

  18. 75 FR 13329 - Implications of Financial Accounting System (FAS) 166 on SBA Guaranteed Loan Programs

    Science.gov (United States)

    2010-03-19

    ... ADMINISTRATION Implications of Financial Accounting System (FAS) 166 on SBA Guaranteed Loan Programs AGENCY... Administration (SBA) is soliciting information and views from the public on: (1) The effect that the accounting changes mandated by the Financial Accounting Standards Board (FASB) in Financial Accounting Standard...

  19. 75 FR 65615 - Conditional Commitment for a Federal Loan Guarantee for Project Financing for Southwest Intertie...

    Science.gov (United States)

    2010-10-26

    ... issue loan guarantees in support of debt financing for transmission infrastructure investment projects... for SWIP South and SWIP North were specified in the ROD issued by BLM on December 14, 1994 (59 FR... Agency on February 19, 2010 (75 FR 7479). Western did not recirculate the SWIP EIS or SWIP South EA as...

  20. 76 FR 13304 - Benefits Payable in Terminated Single-Employer Plans; Limitations on Guaranteed Benefits

    Science.gov (United States)

    2011-03-11

    ... category to which a particular benefit is assigned in the asset allocation can affect insurance program... asset allocation is particularly important, because it often includes benefits that, depending on the... Certain Purposes; Guaranteed Benefits; Allocation of Plan Assets; Pension Protection Act of 2006,''...

  1. A Unified Pricing of Variable Annuity Guarantees under the Optimal Stochastic Control Framework

    Directory of Open Access Journals (Sweden)

    Pavel V. Shevchenko

    2016-07-01

    Full Text Available In this paper, we review pricing of the variable annuity living and death guarantees offered to retail investors in many countries. Investors purchase these products to take advantage of market growth and protect savings. We present pricing of these products via an optimal stochastic control framework and review the existing numerical methods. We also discuss pricing under the complete/incomplete financial market models, stochastic mortality and optimal/sub-optimal policyholder behavior, and in the presence of taxes. For numerical valuation of these contracts in the case of simple risky asset process, we develop a direct integration method based on the Gauss-Hermite quadratures with a one-dimensional cubic spline for calculation of the expected contract value, and a bi-cubic spline interpolation for applying the jump conditions across the contract cashflow event times. This method is easier to implement and faster when compared to the partial differential equation methods if the transition density (or its moments of the risky asset underlying the contract is known in closed form between the event times. We present accurate numerical results for pricing of a Guaranteed Minimum Accumulation Benefit (GMAB guarantee available on the market that can serve as a numerical benchmark for practitioners and researchers developing pricing of variable annuity guarantees to assess the accuracy of their numerical implementation.

  2. Guarantee of supply in the Spanish electricity industry has improved substantially

    International Nuclear Information System (INIS)

    The president of the National Energy Commission talks about the Spanish electricity and gas management system, its weaknesses and the need to progress towards a European single energy market model allowing supplies to be guaranteed in all the countries of the European Union. (Author)

  3. Massive M2M Access with Reliability Guarantees in LTE Systems

    DEFF Research Database (Denmark)

    Madueño, Germán Corrales; Kiilerich Pratas, Nuno; Stefanovic, Cedomir;

    2015-01-01

    Machine-to-Machine (M2M) communications are one of the major drivers of the cellular network evolution towards 5G systems. One of the key challenges is on how to provide reliability guarantees to each accessing device in a situation in which there is a massive number of almost-simultaneous arrivals...

  4. Guaranteed Tax Base Formulas in School Finance: Can Equalization Be Achieved? Reply to Phelps and Addonizio.

    Science.gov (United States)

    Reilly, Gilbert J.

    1982-01-01

    Among the author's replies to the preceding article are that property tax relief programs do not solve interdistrict inequities under guaranteed tax base formulas, district income better measures fiscal capacity than does property wealth, and intrastate cost differentials do not remedy problems of low-income districts in high-income areas. (RW)

  5. GTB: An Aid in Understanding Connecticut's Guaranteed Tax Base Grant/School Finance Equalization Program.

    Science.gov (United States)

    Connecticut State Dept. of Education, Hartford.

    This booklet discusses the Horton v. Meskill case in which the Hartford, Connecticut, Superior Court ruled that the state's school financing system did not comply with state constitutional provisions concerning equal protection and education. It then outlines the legislature's reaction to the ruling--the guaranteed tax base (GTB)--and discusses…

  6. Wealth Neutrality Revisited: Guaranteed Tax Base Formulas and Local Choice in Public Education.

    Science.gov (United States)

    Reilly, Gilbert J.

    1982-01-01

    Proposes a model for evaluating state equalization aid formulas, accounting for municipal overburden and tradeoffs between expenditures and taxes. Tests the model using the Michigan state aid formula. Results relate elasticities to spending and tax levels and suggest that relatively unrestricted guaranteed tax base formulas can work. (PGD)

  7. Guaranteed Tax Base Formulas in School Finance: Why Equalization Doesn't Work.

    Science.gov (United States)

    Reilly, Gilbert J.

    1982-01-01

    Two shortcomings in the implementation of guaranteed tax base or equalization formulas--limitations on state equalization aid and inaccurate portrayal of fiscal capacity--can maintain or increase financial disparities among school districts. Data on Michigan districts illustrate how inaccurate fiscal measurement expanded existing disparities…

  8. 13 CFR 107.1600 - SBA authority to issue and guarantee Trust Certificates.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false SBA authority to issue and guarantee Trust Certificates. 107.1600 Section 107.1600 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES SBA Financial Assistance for Licensees (Leverage)...

  9. An LMI Method to Robust Iterative Learning Fault-tolerant Guaranteed Cost Control for Batch Processes

    Institute of Scientific and Technical Information of China (English)

    WANG Limin; CHEN Xi; GAO Furong

    2013-01-01

    Based on an equivalent two-dimensional Fornasini-Marchsini model for a batch process in industry,a closed-loop robust iterative learning fault-tolerant guaranteed cost control scheme is proposed for batch processes with actuator failures.This paper introduces relevant concepts of the fault-tolerant guaranteed cost control and formulates the robust iterative learning reliable guaranteed cost controller (ILRGCC).A significant advantage is that the proposed ILRGCC design method can be used for on-line optimization against batch-to-batch process uncertainties to realize robust tracking of set-point trajectory in time and batch-to-batch sequences.For the convenience of implementation,only measured output errors of current and previous cycles are used to design a synthetic controller for iterative learning control,consisting of dynamic output feedback plus feed-forward control.The proposed controller can not only guarantee the closed-loop convergency along time and cycle sequences but also satisfy the H∞ performance level and a cost function with upper bounds for all admissible uncertainties and any actuator failures.Sufficient conditions for the controller solution are derived in terms of linear matrix inequalities (LMIs),and design procedures,which formulate a convex optimization problem with LMI constraints,are presented.An example of injection molding is given to illustrate the effectiveness and advantages of the ILRGCC design approach.

  10. 76 FR 2571 - Surety Bond Guarantee Program; Disaster and Miscellaneous Amendments

    Science.gov (United States)

    2011-01-14

    ...The U.S. Small Business Administration (SBA) is issuing this final rule to implement the authority provided by the Small Business Disaster Response and Loan Improvements Act of 2008, for issuing surety bond guarantees for contracts and orders related to a major disaster. The rule also clarifies that the SBA does not cover any costs related to any insurance or indemnification requirements in......

  11. 48 CFR 52.247-8 - Estimated Weights or Quantities Not Guaranteed.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Estimated Weights or... Provisions and Clauses 52.247-8 Estimated Weights or Quantities Not Guaranteed. As prescribed in 47.207-3(e... transportation-related services when weights or quantities are estimates: Estimated Weights or Quantities...

  12. One in a Hundred – the third year of the development guarantee

    OpenAIRE

    Angelin, Anna

    2000-01-01

    Summary of report No 23. The Development Guarantee was introduced in Sweden January 1, 1998. It gives municipalities the opportunity to assume responsibility for young people between the ages of 20 to 24 for which the employment office cannot find employment within 90 days. 2000.

  13. 75 FR 20257 - Amendment of the Temporary Liquidity Guarantee Program To Extend the Transaction Account...

    Science.gov (United States)

    2010-04-19

    ... qualify for the FDIC's guarantee and to continue to exercise risk- management principles applicable to an... deposit insurance limit) in qualifying noninterest-bearing transaction accounts. \\2\\ 73 FR 64179 (Oct. 29... assessment, ranging from 15 to 25 basis points based on an institution's deposit insurance assessment...

  14. 20 CFR 404.242 - Use of old-start primary insurance amount as guaranteed alternative.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Use of old-start primary insurance amount as guaranteed alternative. 404.242 Section 404.242 Employees' Benefits SOCIAL SECURITY ADMINISTRATION FEDERAL OLD-AGE, SURVIVORS AND DISABILITY INSURANCE (1950- ) Computing Primary Insurance Amounts...

  15. Controlling Chaos in Permanent Magnet Synchronous Motor Control System via Fuzzy Guaranteed Cost Controller

    OpenAIRE

    Yi-You Hou

    2012-01-01

    This paper investigates the guaranteed cost control of chaos problem in permanent magnet synchronous motor (PMSM) via Takagi-Sugeno (T-S) fuzzy method approach. Based on Lyapunov stability theory and linear matrix inequality (LMI) technique, a state feedback controller is proposed to stabilize the PMSM systems. An illustrative example is provided to verify the validity of the results developed in this paper.

  16. Nonuniform covering method as applied to multicriteria optimization problems with guaranteed accuracy

    Science.gov (United States)

    Evtushenko, Yu. G.; Posypkin, M. A.

    2013-02-01

    The nonuniform covering method is applied to multicriteria optimization problems. The ɛ-Pareto set is defined, and its properties are examined. An algorithm for constructing an ɛ-Pareto set with guaranteed accuracy ɛ is described. The efficiency of implementing this approach is discussed, and numerical results are presented.

  17. Heme Recognition By a Staphylococcus Aureus IsdE

    Energy Technology Data Exchange (ETDEWEB)

    Grigg, J.C.; Vermeiren, C.L.; Heinrichs, D.E.; Murphy, M.E.P.

    2009-06-03

    Staphylococcus aureus is a Gram-positive bacterial pathogen and a leading cause of hospital acquired infections. Because the free iron concentration in the human body is too low to support growth, S. aureus must acquire iron from host sources. Heme iron is the most prevalent iron reservoir in the human body and a predominant source of iron for S. aureus. The iron-regulated surface determinant (Isd) system removes heme from host heme proteins and transfers it to IsdE, the cognate substrate-binding lipoprotein of an ATP-binding cassette transporter, for import and subsequent degradation. Herein, we report the crystal structure of the soluble portion of the IsdE lipoprotein in complex with heme. The structure reveals a bi-lobed topology formed by an N- and C-terminal domain bridged by a single {alpha}-helix. The structure places IsdE as a member of the helical backbone metal receptor superfamily. A six-coordinate heme molecule is bound in the groove established at the domain interface, and the heme iron is coordinated in a novel fashion for heme transporters by Met{sup 78} and His{sup 229}. Both heme propionate groups are secured by H-bonds to IsdE main chain and side chain groups. Of these residues, His{sup 299} is essential for IsdE-mediated heme uptake by S. aureus when growth on heme as a sole iron source is measured. Multiple sequence alignments of homologues from several other Gram-positive bacteria, including the human pathogens pyogenes, Bacillus anthracis, and Listeria monocytogenes, suggest that these other systems function equivalently to S. aureus IsdE with respect to heme binding and transport.

  18. Characterization of a mouse-adapted Staphylococcus aureus strain.

    Science.gov (United States)

    Holtfreter, Silva; Radcliff, Fiona J; Grumann, Dorothee; Read, Hannah; Johnson, Sarah; Monecke, Stefan; Ritchie, Stephen; Clow, Fiona; Goerke, Christiane; Bröker, Barbara M; Fraser, John D; Wiles, Siouxsie

    2013-01-01

    More effective antibiotics and a protective vaccine are desperately needed to combat the 'superbug' Staphylococcus aureus. While in vivo pathogenicity studies routinely involve infection of mice with human S. aureus isolates, recent genetic studies have demonstrated that S. aureus lineages are largely host-specific. The use of such animal-adapted S. aureus strains may therefore be a promising approach for developing more clinically relevant animal infection models. We have isolated a mouse-adapted S. aureus strain (JSNZ) which caused a severe outbreak of preputial gland abscesses among male C57BL/6J mice. We aimed to extensively characterize this strain on a genomic level and determine its virulence potential in murine colonization and infection models. JSNZ belongs to the MLST type ST88, rare among human isolates, and lacks an hlb-converting phage encoding human-specific immune evasion factors. Naive mice were found to be more susceptible to nasal and gastrointestinal colonization with JSNZ than with the human-derived Newman strain. Furthermore, naïve mice required antibiotic pre-treatment to become colonized with Newman. In contrast, JSNZ was able to colonize mice in the absence of antibiotic treatment suggesting that this strain can compete with the natural flora for space and nutrients. In a renal abscess model, JSNZ caused more severe disease than Newman with greater weight loss and bacterial burden. In contrast to most other clinical isolates, JSNZ can also be readily genetically modified by phage transduction and electroporation. In conclusion, the mouse-adapted strain JSNZ may represent a valuable tool for studying aspects of mucosal colonization and for screening novel vaccines and therapies directed at preventing colonization. PMID:24023720

  19. Staphylococcus aureus phage types and their correlation to antibiotic resistance

    Directory of Open Access Journals (Sweden)

    Mehndiratta P

    2010-10-01

    Full Text Available Context: Staphylococcus aureus is one of the most devastating human pathogen. The organism has a differential ability to spread and cause outbreak of infections. Characterization of these strains is important to control the spread of infection in the hospitals as well as in the community. Aim: To identify the currently existing phage groups of Staphylococcus aureus, their prevalence and resistance to antibiotics. Materials and Methods: Study was undertaken on 252 Staphylococcus aureus strains isolated from clinical samples. Strains were phage typed and their resistance to antibiotics was determined following standard microbiological procedures. Statistical Analysis: Chi square test was used to compare the antibiotic susceptibility between methicillin resistant Staph. aureus (MRSA and methicillin sensitive S. aureus (MSSA strains. Results: Prevalence of MRSA and MSSA strains was found to be 29.36% and 70.65% respectively. Of these 17.56% of MRSA and 40.44% of MSSA strains were community acquired. All the MSSA strains belonging to phage type 81 from the community were sensitive to all the antibiotics tested including clindamycin and were resistant to penicillin. Forty five percent strains of phage group III and 39% of non-typable MRSA strains from the hospital were resistant to multiple antibiotics. Conclusion: The study revealed that predominant phage group amongst MRSA strains was phage group III and amongst MSSA from the community was phage group NA (phage type 81. MSSA strains isolated from the community differed significantly from hospital strains in their phage type and antibiotic susceptibility. A good correlation was observed between community acquired strains of phage type 81 and sensitivity to gentamycin and clindamycin.

  20. Characterization of a mouse-adapted Staphylococcus aureus strain.

    Directory of Open Access Journals (Sweden)

    Silva Holtfreter

    Full Text Available More effective antibiotics and a protective vaccine are desperately needed to combat the 'superbug' Staphylococcus aureus. While in vivo pathogenicity studies routinely involve infection of mice with human S. aureus isolates, recent genetic studies have demonstrated that S. aureus lineages are largely host-specific. The use of such animal-adapted S. aureus strains may therefore be a promising approach for developing more clinically relevant animal infection models. We have isolated a mouse-adapted S. aureus strain (JSNZ which caused a severe outbreak of preputial gland abscesses among male C57BL/6J mice. We aimed to extensively characterize this strain on a genomic level and determine its virulence potential in murine colonization and infection models. JSNZ belongs to the MLST type ST88, rare among human isolates, and lacks an hlb-converting phage encoding human-specific immune evasion factors. Naive mice were found to be more susceptible to nasal and gastrointestinal colonization with JSNZ than with the human-derived Newman strain. Furthermore, naïve mice required antibiotic pre-treatment to become colonized with Newman. In contrast, JSNZ was able to colonize mice in the absence of antibiotic treatment suggesting that this strain can compete with the natural flora for space and nutrients. In a renal abscess model, JSNZ caused more severe disease than Newman with greater weight loss and bacterial burden. In contrast to most other clinical isolates, JSNZ can also be readily genetically modified by phage transduction and electroporation. In conclusion, the mouse-adapted strain JSNZ may represent a valuable tool for studying aspects of mucosal colonization and for screening novel vaccines and therapies directed at preventing colonization.

  1. Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection.

    Directory of Open Access Journals (Sweden)

    Aisling F Brown

    Full Text Available Mechanisms of protective immunity to Staphylococcus aureus infection in humans remain elusive. While the importance of cellular immunity has been shown in mice, T cell responses in humans have not been characterised. Using a murine model of recurrent S. aureus peritonitis, we demonstrated that prior exposure to S. aureus enhanced IFNγ responses upon subsequent infection, while adoptive transfer of S. aureus antigen-specific Th1 cells was protective in naïve mice. Translating these findings, we found that S. aureus antigen-specific Th1 cells were also significantly expanded during human S. aureus bloodstream infection (BSI. These Th1 cells were CD45RO+, indicative of a memory phenotype. Thus, exposure to S. aureus induces memory Th1 cells in mice and humans, identifying Th1 cells as potential S. aureus vaccine targets. Consequently, we developed a model vaccine comprising staphylococcal clumping factor A, which we demonstrate to be an effective human T cell antigen, combined with the Th1-driving adjuvant CpG. This novel Th1-inducing vaccine conferred significant protection during S. aureus infection in mice. This study notably advances our understanding of S. aureus cellular immunity, and demonstrates for the first time that a correlate of S. aureus protective immunity identified in mice may be relevant in humans.

  2. Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection.

    Science.gov (United States)

    Brown, Aisling F; Murphy, Alison G; Lalor, Stephen J; Leech, John M; O'Keeffe, Kate M; Mac Aogáin, Micheál; O'Halloran, Dara P; Lacey, Keenan A; Tavakol, Mehri; Hearnden, Claire H; Fitzgerald-Hughes, Deirdre; Humphreys, Hilary; Fennell, Jérôme P; van Wamel, Willem J; Foster, Timothy J; Geoghegan, Joan A; Lavelle, Ed C; Rogers, Thomas R; McLoughlin, Rachel M

    2015-01-01

    Mechanisms of protective immunity to Staphylococcus aureus infection in humans remain elusive. While the importance of cellular immunity has been shown in mice, T cell responses in humans have not been characterised. Using a murine model of recurrent S. aureus peritonitis, we demonstrated that prior exposure to S. aureus enhanced IFNγ responses upon subsequent infection, while adoptive transfer of S. aureus antigen-specific Th1 cells was protective in naïve mice. Translating these findings, we found that S. aureus antigen-specific Th1 cells were also significantly expanded during human S. aureus bloodstream infection (BSI). These Th1 cells were CD45RO+, indicative of a memory phenotype. Thus, exposure to S. aureus induces memory Th1 cells in mice and humans, identifying Th1 cells as potential S. aureus vaccine targets. Consequently, we developed a model vaccine comprising staphylococcal clumping factor A, which we demonstrate to be an effective human T cell antigen, combined with the Th1-driving adjuvant CpG. This novel Th1-inducing vaccine conferred significant protection during S. aureus infection in mice. This study notably advances our understanding of S. aureus cellular immunity, and demonstrates for the first time that a correlate of S. aureus protective immunity identified in mice may be relevant in humans. PMID:26539822

  3. INSURANCE GUARANTEE

    OpenAIRE

    Stanescu Catalin-Florinel; Simion Mircea-Laurentiu

    2013-01-01

    Providing security is designed to reduce uncertainty in the credit granting, offers a service or goods entrusted to a third persoane.Acest type of financial risk insurance is different from celelelte by the person who pays the insurance premium is not entitled direct insurance.

  4. Sensitive and rapid detection of staphylococcus aureus in milk via cell binding domain of lysin.

    Science.gov (United States)

    Yu, Junping; Zhang, Yun; Zhang, Yun; Li, Heng; Yang, Hang; Wei, Hongping

    2016-03-15

    Staphylococcus aureus (S. aureus) is an important food-borne pathogen in dairy products contaminated through raw ingredients or improper food handling. Rapid detection of S. aureus with high sensitivity is of significance for food quality and safety. In this study, a new method was developed for detecting S. aureus in milk by coupling immunomagnetic separation with enzyme linked cell wall binding domain (CBD) of lysin plyV12, which can bind to S. aureus with high affinity. There are millions of binding sites present on the cell surface of S. aureus for the CBD attachment, which greatly improves the detection sensitivity. The method has the overall testing time of only 1.5h with the detection limit of 4 × 10(3)CFU/mL in spiked milk. Because it is simple, rapid and sensitive, this method could be used for the detection of S. aureus in various food samples. PMID:26433070

  5. Antibiotic-mediated selection of quorum-sensing-negative Staphylococcus aureus

    DEFF Research Database (Denmark)

    Paulander, Wilhelm Erik Axel; Varming, Anders Nissen; Bæk, Kristoffer Torbjørn;

    2012-01-01

    -acquired S. aureus infections and suggest that the adaptability of S. aureus to antibiotics involves the agr locus. IMPORTANCE: Staphylococcus aureus is the most frequently isolated pathogen in intensive care units and a common cause of nosocomial infections, resulting in a high degree of morbidity and......Staphylococcus aureus is a human commensal that at times turns into a serious bacterial pathogen causing life-threatening infections. For the delicate control of virulence, S. aureus employs the agr quorum-sensing system that, via the intracellular effector molecule RNAIII, regulates virulence gene...... increases the agr-mediated fitness cost by inducing the expression of RNAIII. Thus, the extensive use of antibiotics in hospitals may explain why agr-negative variants are frequently isolated from hospital-acquired S. aureus infections but rarely found among community-acquired S. aureus strains. Importantly...

  6. Shedding of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus from adult and pediatric bathers in marine waters

    Directory of Open Access Journals (Sweden)

    Sinigalliano Christopher D

    2011-01-01

    Full Text Available Abstract Background Staphylococcus aureus including methicillin resistant S. aureus, MRSA, are human colonizing bacteria that commonly cause opportunistic infections primarily involving the skin in otherwise healthy individuals. These infections have been linked to close contact and sharing of common facilities such as locker rooms, schools and prisons Waterborne exposure and transmission routes have not been traditionally associated with S. aureus infections. Coastal marine waters and beaches used for recreation are potential locations for the combination of high numbers of people with close contact and therefore could contribute to the exposure to and infection by these organisms. The primary aim of this study was to evaluate the amount and characteristics of the shedding of methicillin sensitive S. aureus, MSSA and MRSA by human bathers in marine waters. Results Nasal cultures were collected from bathers, and water samples were collected from two sets of pools designed to isolate and quantify MSSA and MRSA shed by adults and toddlers during exposure to marine water. A combination of selective growth media and biochemical and polymerase chain reaction analysis was used to identify and perform limited characterization of the S. aureus isolated from the water and the participants. Twelve of 15 MRSA isolates collected from the water had identical genetic characteristics as the organisms isolated from the participants exposed to that water while the remaining 3 MRSA were without matching nasal isolates from participants. The amount of S. aureus shed per person corresponded to 105 to 106 CFU per person per 15-minute bathing period, with 15 to 20% of this quantity testing positive for MRSA. Conclusions This is the first report of a comparison of human colonizing organisms with bacteria from human exposed marine water attempting to confirm that participants shed their own colonizing MSSA and MRSA into their bathing milieu. These findings clearly

  7. 29 CFR 778.412 - Relationship between amount guaranteed and range of hours employee may be expected to work.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Relationship between amount guaranteed and range of hours... Compensation Which Includes Overtime Pay § 778.412 Relationship between amount guaranteed and range of hours... workweeks. In both Walling v. A. H. Belo Co., 316 U.S. 624 and Walling v. Halliburton Oil Well Cementing...

  8. 75 FR 3197 - Notice of a Public Meeting on Administration of the Business and Industry Guaranteed Loan Program

    Science.gov (United States)

    2010-01-20

    ... Industry Guaranteed Loan Program AGENCY: Rural Business-Cooperative Service, USDA. ACTION: Notice of public... Development Mission area, will hold a public meeting entitled ``Business and Industry Guaranteed Loan Program... Hyatt Regency DFW Airport Hotel from 8 a.m. to 5 p.m. CST. ADDRESSES: The public meeting will be held...

  9. 78 FR 56227 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-09-12

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this Transaction....

  10. 78 FR 78955 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-12-27

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... application for final commitment for a long-term loan or financial guarantee in excess of $100 million (as... specified below will be presented to the Ex-Im Bank Board of Directors prior to final action on...

  11. 78 FR 69850 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-11-21

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this Transaction....

  12. 78 FR 42776 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-07-17

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this...

  13. 78 FR 23763 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-04-22

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this...

  14. 78 FR 2673 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-01-14

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... final commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in... will be presented to the Ex-Im Bank Board of Directors prior to final action on this...

  15. 78 FR 1211 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-01-08

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this...

  16. 77 FR 47382 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2012-08-08

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this Transaction....

  17. 78 FR 21948 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-04-12

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this...

  18. 78 FR 39285 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-07-01

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this...

  19. 78 FR 76614 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-12-18

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this Transaction....

  20. 78 FR 2672 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-01-14

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... application for final commitment for a long-term loan or financial guarantee in excess of $100 million (as... specified below will be presented to the Ex-Im Bank Board of Directors prior to final action on...

  1. 78 FR 59688 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-09-27

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... final commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in... will be presented to the Ex-Im Bank Board of Directors prior to final action on this Transaction....

  2. 78 FR 16852 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-03-19

    ... UNITED STATES Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of... commitment for a long-term loan or financial guarantee in excess of $100 million (as calculated in accordance... be presented to the Ex-Im Bank Board of Directors prior to final action on this...

  3. 78 FR 20913 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-04-08

    ... From the Federal Register Online via the Government Publishing Office EXPORT-IMPORT BANK Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100 million... loan or financial guarantee in excess of $100 million (as calculated in accordance with Section...

  4. 78 FR 16675 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-03-18

    ... From the Federal Register Online via the Government Publishing Office EXPORT-IMPORT BANK Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100 Million... loan or financial guarantee in excess of $100 million (as calculated in accordance with Section...

  5. 78 FR 13875 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Science.gov (United States)

    2013-03-01

    ... From the Federal Register Online via the Government Publishing Office EXPORT-IMPORT BANK Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100 Million... loan or financial guarantee in excess of $100 million (as calculated in accordance with Section...

  6. 20 CFR 404.233 - Adjustment of your guaranteed alternative when you become entitled after age 62.

    Science.gov (United States)

    2010-04-01

    ... when you become entitled after age 62. 404.233 Section 404.233 Employees' Benefits SOCIAL SECURITY... Guaranteed Alternative for People Reaching Age 62 After 1978 But Before 1984 § 404.233 Adjustment of your guaranteed alternative when you become entitled after age 62. (a) If you do not become entitled to...

  7. Antimicrobial potential of Pakistani medicinal plants against multi-drug resistance Staphylococcus aureus

    Institute of Scientific and Technical Information of China (English)

    Rahat Ejaz; Usman A Ashfaq; Sobia Idrees

    2014-01-01

    Objective: To determine resistance patterns of Staphylococcus aureus (S. aureus) isolated from different areas of Pakistan and to identify antimicrobial agents against multi-drug resistant S.aureus strains. Methods: A total of 67 samples (sewerage, nasal and milk) were collected from different farm areas of Pakistan to identify local strains of S. aureus. Sixteen out of 67 samples were positive for S.aureus. Only 6 out of 16 S. aureus strains showed resistance to antibiotics. Then the antibacterial effect of 29 medicinal plants was evaluated on these S. aureus isolates and a standard S. aureus strain ATCC 25923. The solvents used for the extraction of plants were acetone, dimethyl sulfoxide and methanol. The in vitro antibacterial activity was performed using agar disc diffusion method. Moreover, minimum inhibitory concentration of effective medicinal plant extracts was identified through micro-dilution method to find out their 50% inhibitory concentration.Results:Plant extracts of 5 medicinal plants (Psidium guajava, Nigella sativa, Piper nigrum, Valeriana jatamansi, and Cucurbita pepo) exhibited antibacterial activity against locally isolated multidrug resistant strains of S. aureus. The minimum inhibitory concentration of these extracts was ranged from 0.328 to 5.000 mg/mL. Conclusions: Plant extracts of Psidium guajava, Piper nigrum seed, Valeriana jatamansi, Cucurbita pepo and Nigella sativa showed significant in vitro antibacterial activity and thus, such findings may serve as valuable contribution in the treatment of infection and may contribute to the development of potential antimicrobial agents against multi drug resistant strains of S. aureus.

  8. Antimicrobial potential of Pakistani medicinal plants against multi-drug resistance Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Rahat Ejaz

    2014-09-01

    Full Text Available Objective: To determine resistance patterns of Staphylococcus aureus (S. aureus isolated from different areas of Pakistan and to identify antimicrobial agents against multi-drug resistant S. aureus strains. Methods: A total of 67 samples (sewerage, nasal and milk were collected from different farm areas of Pakistan to identify local strains of S. aureus. Sixteen out of 67 samples were positive for S. aureus. Only 6 out of 16 S. aureus strains showed resistance to antibiotics. Then the antibacterial effect of 29 medicinal plants was evaluated on these S. aureus isolates and a standard S. aureus strain ATCC 25923. The solvents used for the extraction of plants were acetone, dimethyl sulfoxide and methanol. The in vitro antibacterial activity was performed using agar disc diffusion method. Moreover, minimum inhibitory concentration of effective medicinal plant extracts was identified through micro-dilution method to find out their 50% inhibitory concentration. Results: Plant extracts of 5 medicinal plants (Psidium guajava, Nigella sativa, Piper nigrum, Valeriana jatamansi, and Cucurbita pepo exhibited antibacterial activity against locally isolated multidrug resistant strains of S. aureus. The minimum inhibitory concentration of these extracts was ranged from 0.328 to 5.000 mg/mL. Conclusions: Plant extracts of Psidium guajava, Piper nigrum seed, Valeriana jatamansi, Cucurbita pepo and Nigella sativa showed significant in vitro antibacterial activity and thus, such findings may serve as valuable contribution in the treatment of infection and may contribute to the development of potential antimicrobial agents against multi drug resistant strains of S. aureus

  9. Portación de Staphylococcus aureus enterotoxigénicos en manipuladores de alimentos Carriage of enterotoxigenic Staphylococcus aureus in food handlers

    Directory of Open Access Journals (Sweden)

    Guillermo Figueroa G

    2002-08-01

    Full Text Available Background: One of the most common pathogens causing alimentary toxi-infections is Staphylococcus aureus (S aureus. The presence of S aureus in food, indicates flaws during food manipulations. For this reason most sanitary norms require the detection of S aureus carriers. Aim: To determine the carriage rate of enterotoxin producing S aureus strains in food handlers, and to evaluate the antibiotic susceptibility to six antimicrobial agents. Materials and Methods: A total of 102 food handlers from 19 restaurants in Santiago, were analyzed. Samples for microbiological analysis were obtained with a swab from the retropharynx. Results: S aureus grew in 35 out of the 102 samples obtained (34%. Further analysis revealed that 19/35 (54% strains were able to produce enterotoxins. Therefore the corrected carriage rate was 19% (19/102. The most frequently detected enterotoxin was the type A (12/19. All S aureus isolates were resistant to penicillin and susceptible to oxacillin, clindamycin, kanamycin, vancomycin and linezolid. Conclusions: The carriage rate of S aureus in food handlers is similar to the rate reported in the general population in our country. These results confirm the need for education and training programs in food safety, directed to food handlers (Rev Méd Chile 2002; 130: 859-64

  10. Mastitis Bovina: Resistencia a antibióticos de cepas de Staphylococcus aureus asiladas de leche (Bovine Mastitis: Antimicrobial resistance of Staphylococcus aureus strains isolated from milk)

    OpenAIRE

    Pellegrino, MS; Frola, ID; Odierno, LM; Bogni, CI

    2011-01-01

    ResumenLa mastitis bovina es considerada la enfermedad infecciosa del ganado lechero de mayor impacto económico mundial, siendo Staphylococcus aureus el principal agente patógeno en muchos países.SummaryBovine mastitis is a frequent cause of economic loss in worldwide dairy herds, being Staphylococcus aureus the main etiological agent in many countries.

  11. Mastitis Bovina: Resistencia a antibióticos de cepas de Staphylococcus aureus asiladas de leche (Bovine Mastitis: Antimicrobial resistance of Staphylococcus aureus strains isolated from milk

    Directory of Open Access Journals (Sweden)

    Pellegrino, MS

    2011-07-01

    Full Text Available ResumenLa mastitis bovina es considerada la enfermedad infecciosa del ganado lechero de mayor impacto económico mundial, siendo Staphylococcus aureus el principal agente patógeno en muchos países.SummaryBovine mastitis is a frequent cause of economic loss in worldwide dairy herds, being Staphylococcus aureus the main etiological agent in many countries.

  12. Cavity Forming Pneumonia Due to Staphylococcus aureus Following Dengue Fever.

    Science.gov (United States)

    Miyata, Nobuyuki; Yoshimura, Yukihiro; Tachikawa, Natsuo; Amano, Yuichiro; Sakamoto, Yohei; Kosuge, Youko

    2015-11-01

    While visiting Malaysia, a 22-year-old previously healthy Japanese man developed myalgia, headache, and fever, leading to a diagnosis of classical dengue fever. After improvement and returning to Japan after a five day hospitalization, he developed productive cough several days after defervescing from dengue. Computed tomography (CT) thorax scan showed multiple lung cavities. A sputum smear revealed leukocytes with phagocytized gram-positive cocci in clusters, and grew an isolate Staphylococcus aureus sensitive to semi-synthetic penicillin; he was treated successfully with ceftriaxone and cephalexin. This second reported case of pneumonia due to S. aureus occurring after dengue fever, was associated both with nosocomial exposure and might have been associated with dengue-associated immunosuppression. Clinicians should pay systematic attention to bacterial pneumonia following dengue fever to establish whether such a connection is causally associated. PMID:26304914

  13. Optimal Guaranteed Service Time and Service Level Decision with Time and Service Level Sensitive Demand

    Directory of Open Access Journals (Sweden)

    Sangjun Park

    2014-01-01

    Full Text Available We consider a two-stage supply chain with one supplier and one retailer. The retailer sells a product to customer and the supplier provides a product in a make-to-order mode. In this case, the supplier’s decisions on service time and service level and the retailer’s decision on retail price have effects on customer demand. We develop optimization models to determine the optimal retail price, the optimal guaranteed service time, the optimal service level, and the optimal capacity to maximize the expected profit of the whole supply chain. The results of numerical experiments show that it is more profitable to determine the optimal price, the optimal guaranteed service time, and the optimal service level simultaneously and the proposed model is more profitable in service level sensitive market.

  14. GRS - Guarantee of results for grid-connected solar photovoltaic systems; GRS - Garantierte Resultate von Solarstromanlagen

    Energy Technology Data Exchange (ETDEWEB)

    Meier, Ch.; Frei, R.

    2001-07-01

    This final report for the Swiss Federal Office of Energy describes the development of a performance guarantee for the power delivered by grid-connected photovoltaic (PV) power plant. This is important for investors, who need to be able to calculate the price of the energy produced by the PV plant. The report examines the results of various case studies and experience gained with GRS contracts developed in different countries including Switzerland, the Netherlands, France and Germany. The problems encountered, which provided feedback for the further development of the contract presented in the report, are discussed. The model contract, which proposes a framework which provides many possible variations and adaptations, is presented in detail. Various problem areas such as guaranteed energy yield, meteorological references, correction methods, failure detection, dispute settlement and many other points are covered.

  15. European beef consumers' interest in a beef eating-quality guarantee

    DEFF Research Database (Denmark)

    Verbeke, Wim; Van Wezemael, Lyn; de Barcellos, Marcia Dutra;

    2010-01-01

    Consumer demand in relation to food is increasingly shifting towards products that are safe, nutritious, and of good eating quality. Beef consumers are demanding for experience quality that matches their quality expectations formed prior to consumption, particularly with respect to beef tenderness......, United Kingdom and Germany to assess their opinions about beef muscle profiling and their interest in a beef eating-quality guarantee. Findings indicate that both concepts are well accepted by European beef consumers, although not unconditional. Besides acknowledging the opportunity to purchase beef with...... insights obtained from this study indicate good opportunities for the development of a beef eating-quality guarantee system in Europe. As an increase in consumers' satisfaction with beef products could lead to higher consumption rates and industry profitability, the introduction of an eating...

  16. Decentralized guaranteed cost static output feedback vibration control for piezoelectric smart structures

    International Nuclear Information System (INIS)

    This paper is devoted to the study of the decentralized guaranteed cost static output feedback vibration control for piezoelectric smart structures. A smart panel with collocated piezoelectric actuators and velocity sensors is modeled using a finite element method, and then the size of the model is reduced in the state space using the modal Hankel singular value. The necessary and sufficient conditions of decentralized guaranteed cost static output feedback control for the reduced system have been presented. The decentralized and centralized static output feedback matrices can be obtained from solving two linear matrix inequalities. A comparison between centralized control and decentralized control is performed in order to investigate their effectiveness in suppressing vibration of a smart panel. Numerical results show that when the system is subjected to initial displacement or white noise disturbance, the decentralized and centralized controls are both very effective and the control results are very close

  17. Guaranteed Cost H∞ Controller Synthesis for Switched Systems Defined on Semi-algebraic Sets

    DEFF Research Database (Denmark)

    Ahmadi, Mohamadreza; Mojallali, Hamed; Wisniewski, Rafael

    2014-01-01

    A methodology to design guaranteed cost H∞ controllers for a class of switched systems with polynomial vector fields is proposed. To this end, we use sum of squares programming techniques. In addition, instead of the customary Carathéodory solutions, the analysis is performed in the framework...... feasibility tests leading to a stabilizing switching controller. Finally, we propose a scheme to synthesize stabilizing switching controllers with a guaranteed cost H∞ disturbance attenuation performance. The applicability of the proposed methods is elucidated thorough simulation analysis....... of Filippov solutions which subsumes solutions with infinite switching in finite time and sliding modes. Firstly, conditions assuring asymptotic stability of Filippov solutions pertained to a switched system defined on semi-algebraic sets are formulated. Accordingly, we derive a set of sum of squares...

  18. Development Concept of Guaranteed Verification Electric Power System Simulation Tools and Its Realization

    Directory of Open Access Journals (Sweden)

    Gusev Alexander

    2015-01-01

    Full Text Available The analysis of existing problem reliability and verification of widespread electric power systems (EPS simulation tools is presented in this article. Everything simulation tools are based on the using of numerical methods for ordinary differential equations. Described the concept of guaranteed verification EPS simulation tools and the structure of its realization are based using the Simulator having properties of continuous , without decomposition three-phase EPS simulation in real time and on an unlimited range with guaranteed accuracy. The information from the Simulator can be verified by using data only quasi-steady-state regime received from the SCADA and such Simulator can be applied as the standard model for verification any EPS simulation tools.

  19. Administrative Circular No. 13 (Rev. 3) - Guarantees for representatives of the personnel

    CERN Multimedia

    2014-01-01

    Administrative Circular No. 13 (Rev. 3) entitled “Guarantees for representatives of the personnel”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 5 December 2013 and entering into force on 1 January 2014, is available on the intranet site of the Human Resources Department (see here).   This circular is applicable to all members of the personnel. It cancels and replaces Administrative Circular No. 13 (Rev. 2) entitled “Guarantees for members of the personnel holding representative office” of November 1992. The circular was revised in order to adapt the time given to the representatives of the personnel to perform their elective mandate and to ensure more transparency in their activities, by indicating, inter alia, the percentage of time worked in the framework of their mandate, as well as the training, activities and ensuing results. Department Head Office HR Department

  20. Guaranteed Cost Fault-Tolerant Control for Networked Control Systems with Sensor Faults

    Directory of Open Access Journals (Sweden)

    Qixin Zhu

    2015-01-01

    Full Text Available For the large scale and complicated structure of networked control systems, time-varying sensor faults could inevitably occur when the system works in a poor environment. Guaranteed cost fault-tolerant controller for the new networked control systems with time-varying sensor faults is designed in this paper. Based on time delay of the network transmission environment, the networked control systems with sensor faults are modeled as a discrete-time system with uncertain parameters. And the model of networked control systems is related to the boundary values of the sensor faults. Moreover, using Lyapunov stability theory and linear matrix inequalities (LMI approach, the guaranteed cost fault-tolerant controller is verified to render such networked control systems asymptotically stable. Finally, simulations are included to demonstrate the theoretical results.

  1. Improving the Quality of Service Guarantee in an Asynchronous Transfer Mode (ATM Network

    Directory of Open Access Journals (Sweden)

    Francis J. Ogwu

    2007-01-01

    Full Text Available This study presents a technique for improving the quality of service (QoS guarantee in an ATM network. In the proposed model, it was assumed that high priority traffic have been allocated a switch resource to guarantee a given QoS and low priority cells are allowed to enter the buffer, to improve the exploitation of reserved resources. The proposed technique was backed up with an exact analytical model for evaluating the cell loss probability of high and low priority cells. The performance of the proposed model was evaluated using C++ programming language. The results of the simulation shows that the loss probability of both high and low cells reduces as the buffer capacity increase and that the performance of high priority cell is better than that of low priority cell.

  2. Schistosoma spindale infection in a captive jackal (Canis aureus)

    OpenAIRE

    Vimalraj, P. G.; Latchumikanthan, A.

    2013-01-01

    This report is based on the findings from a captive jackal (Canis aureus) housed in Amirthi Zoological Park, Javadu Hills, Vellore. The animal was reported to be dull, depressed and also had diarrhea. Fecal samples were collected in 10 % formalin and subjected to direct and sedimentation method of faecal examination and was examined for endoparasitic infection. Surprisingly, fecal examination revealed two spindle shaped eggs having terminal spine with a size of 250μ by 60μ. The eggs were iden...

  3. Acral lick dermatitis in a jackal (Canis aureus).

    Science.gov (United States)

    Yeruham, I; Nyska, A

    1998-06-01

    Acral lick dermatitis was diagnosed in a 6-mo-old female jackal (Canis aureus) that was born and housed in a zoological garden in Hafez-Haim, Israel. Other dermatologic diseases were ruled out. Although the lesions were presumed to be psychogenic in origin, they resolved with topical therapy using an ointment containing benzocaine, neomycin sulfate, and hydrocortisone acetate. No recurrence has been observed. PMID:9732044

  4. Methicillin resistant Staphylococcus aureus (MRSA) in the intensive care unit

    OpenAIRE

    Haddadin, A; Fappiano, S; Lipsett, P

    2002-01-01

    Methicillin resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen that causes severe morbidity and mortality worldwide. MRSA strains are endemic in many American and European hospitals and account for 29%–35% of all clinical isolates. Recent studies have documented the increased costs associated with MRSA infection, as well as the importance of colonisation pressure. Surveillance strategies have been proposed especially in high risk areas such as the intensive care unit. Pneum...

  5. CHARACTERIZATION OF HOSPITAL ACQUIRED METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS

    OpenAIRE

    Preethi. B.M; J.Vimalin Hena

    2012-01-01

    Methicillin Resistant Staphylococcus aureus (MRSA) strains have emerged as one of the most important nosocomial pathogens. The MRSA can cause a wide range of diseases, which is associated with its production to large number of extracellular toxins and other virulence factors. The diseases are toxic shock syndrome, scalded skin syndrome and food poisoning. Hospital-acquired MRSA (HA-MRSA) in persons who have had frequent or recent contact with hospitals or healthcare facilities within the prev...

  6. Diversity of Prophages in Dominant Staphylococcus aureus Clonal Lineages▿

    OpenAIRE

    Goerke, Christiane; Pantucek, Roman; Holtfreter, Silva; Schulte, Berit; Zink, Manuel; Grumann, Dorothee; Barbara M. Bröker; Doskar, Jiri; Wolz, Christiane

    2009-01-01

    Temperate bacteriophages play an important role in the pathogenicity of Staphylococcus aureus, for instance, by mediating the horizontal gene transfer of virulence factors. Here we established a classification scheme for staphylococcal prophages of the major Siphoviridae family based on integrase gene polymorphism. Seventy-one published genome sequences of staphylococcal phages were clustered into distinct integrase groups which were related to the chromosomal integration site and to the enco...

  7. Quinupristin/dalfopristin in Staphylococcus aureus endophthalmitis: a case report

    OpenAIRE

    Hernandez-Da Mota Sergio E

    2011-01-01

    Abstract Introduction The intravitreal injection of antibiotics remains the mainstay of therapy for postoperative endophthalmitis. Bacterial resistance, however, is still a pitfall in achieving an adequate response to treatment. Quinupristin/dalfopristin might be a feasible therapeutic option in these cases. Case presentation A 55-year-old Hispanic man had endophthalmitis secondary to Staphylococcus aureus in his right eye and was treated with intravitreal 0.4 mg/0.1 ml quinupristin/dalfopris...

  8. Fatal pneumoni med Panton-Valentine-leukocidinproducerende Staphylococcus aureus

    DEFF Research Database (Denmark)

    Rabøl, Peter Hedelund; Dessau, Ram Benny; Warnecke, Mads;

    2010-01-01

    We describe a case of fatal pneumonia in a previously healthy 14-year-old boy. The patient was severely affected at the time of admission with high fever, tachypnea, tachycardia and peripheral cyanosis. The condition worsened despite treatment with antibiotics as well as respiratory and pressure ...... support. Acidosis and critical leucopenia supervened and the patient died just short of 24 hours after admission. Subsequent bacterial cultivation showed Panton-Valentine Leucocidin-producing Staphylococcus aureus....

  9. Quinolone accumulation in Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus.

    OpenAIRE

    McCaffrey, C; Bertasso, A; Pace, J.; Georgopapadakou, N H

    1992-01-01

    The accumulation of quinolones by Escherichia coli JF568, Pseudomonas aeruginosa PAO1, and Staphylococcus aureus ATCC 29213 was measured by a modified fluorometric assay (J. S. Chapman and N. H. Georgopapadakou, Antimicrob. Agents Chemother. 33:27-29, 1989). The quinolones examined were fleroxacin, pefloxacin, norfloxacin, difloxacin, A56620, ciprofloxacin, ofloxacin, and Ro 09-1168. In all three organisms, uptake was complete in less than 5 min and was proportional to extracellular quinolone...

  10. Bactericidal antibiotic-phytochemical combinations against methicillin resistant Staphylococcus aureus

    OpenAIRE

    Bhone Myint Kyaw; Shuchi arora; Chu Sing Lim

    2012-01-01

    Methicillin resistant Staphylococcus aureus (MRSA) infection is a global concern nowadays. Due to its multi-drug resistant nature, treatment with conventional antibiotics does not assure desired clinical outcomes. Therefore, there is a need to find new compounds and/or alternative methods to get arsenal against the pathogen. Combination therapies using conventional antibiotics and phytochemicals fulfill both requirements. In this study, the efficacy of different phytochemicals in combination ...

  11. Coated vesicle isolation by immunoadsorption on Staphylococcus aureus cells

    OpenAIRE

    1982-01-01

    Porcine brain coated vesicles were isolated from crude fractions of tissue homogenates by affinity separation using anticlathrin-coated STaphylococcus aureus (Staph A) cells as a solid-phase immunoadsorbent. The specificity of the immunoadsorption was monitored by SDS PAGE analysis and by competitive ELISA assays. SDS PAGE of the material immunoadsorbed from a fraction of porcine bran smooth microsomes showed a selective enrichment in a 180,000 mol wt protein. In an ELISA assay, this protein ...

  12. Immunoprophylaxis of Staphylococcus aureus mastitis in diary cows

    OpenAIRE

    Vakanjac Slobodanka; Pavlović M.; Pavlović V.; Obrenović Sonja

    2008-01-01

    Mastitis in cows represents one of the most actual problems in intensive dairy production. The prevention of pathogen penetration in the udder, its colonization and reproduction impose the constant need for regular milk check-ups, and preventive and therapeutic measures. Staphylococcus aureus causes subclinical and clinical mastitis, which when in the acute form can originate difficult and malignant udder infections with granulomatous and necrotic changes. Chronic forms of Staphylococcal mast...

  13. Personal Hygiene and Methicillin-resistant Staphylococcus aureus Infection

    OpenAIRE

    Turabelidze, George; Lin, Mei; Wolkoff, Barbara; Dodson, Douglas; Gladbach, Stephen; Zhu, Bao-Ping

    2006-01-01

    Methicillin-resistant Staphylococcus aureus (MRSA) infections outside the healthcare setting are an increasing concern. We conducted a case-control study to investigate an MRSA outbreak during 2002–2003 in a Missouri prison and focused on hygiene factors. Information on sociodemographic characteristics, medical history, and hygiene practices of study participants was collected by interview and medical record review. Logistic regression was used to evaluate MRSA infection in relation to hygien...

  14. Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridization.

    OpenAIRE

    Dickgiesser, N; Kreiswirth, B N

    1986-01-01

    A method is described for identification of the genes conferring aminoglycoside resistance in Staphylococcus aureus by dot-blot and Southern blot techniques. As radioactive probes, fragments of plasmids pAT48, pUBH2, and pH13, carrying the genes for an aminocyclitol-3'-phosphotransferase, an aminocyclitol-4'-adenylyltransferase, and an aminocyclitol-2''-phosphotransferase-aminocyclitol-6'-acetyltransferase, respectively, were used.

  15. Genetic behavior of the methicillin resistance determinant in Staphylococcus aureus.

    OpenAIRE

    Stewart, G C; Rosenblum, E D

    1980-01-01

    The cotransformation frequency of mecC5 with pur-102 using Staphylococcus aureus C5 deoxyribonucleic acid was found to be approximately 45%. However, in cotransduction studies, there was a 15% cotransduction of purine prototrophy and methicillin sensitivity but, in the reciprocal cross, no purine-prototrophic plus Mecr cotransductants were obtained (frequency less than 0.06%). The data support the hypothesis that the mec determinant resides on an inserted deoxyribonucleic acid sequence in S. ...

  16. Nanoadhesion of Staphylococcus aureus onto Titanium Implant Surfaces

    OpenAIRE

    Aguayo, S.; Donos, N.; Spratt, D.; Bozec, L.

    2015-01-01

    Adhesion of bacteria to dental implant surfaces is the critical initial step in the process of biofilm colonization; however, the specific nanoadhesive interactions occurring during the first contact between bacterial cells and biomaterial substrates remain poorly understood. In this report, we utilize single-cell force spectroscopy to characterize the dynamics of the initial interaction between living Staphylococcus aureus cells and machined titanium surfaces at the nanoscale. Values for max...

  17. Temporal and Stochastic Control of Staphylococcus aureus Biofilm Development

    OpenAIRE

    Moormeier, Derek E.; Bose, Jeffrey L.; Horswill, Alexander R.; Bayles, Kenneth W.

    2014-01-01

    ABSTRACT Biofilm communities contain distinct microniches that result in metabolic heterogeneity and variability in gene expression. Previously, these niches were visualized within Staphylococcus aureus biofilms by observing differential expression of the cid and lrg operons during tower formation. In the present study, we examined early biofilm development and identified two new stages (designated “multiplication” and “exodus”) that were associated with changes in matrix composition and a di...

  18. Stochastic Transfers, Risky Investment and Incomes: Evidence from an Income Guarantee Program in Thailand

    OpenAIRE

    Wagener, Andreas; Zenker, Juliane

    2015-01-01

    From 2009 to 2011, the Thai government implemented an income guarantee program for rice, tapioca and maize farmers. Essentially, this program added a non-negative but stochastic component to the incomes of registered farmers. We evaluate the impact of the program on risk attitudes and investment behavior of small-scale rice farmers in relatively poor North-eastern Thailand. To control for self-selection into the scheme, we use propensity score matching. We find that that participation in the ...

  19. Stochastic transfers, risky investment and incomes: Evidence from an income guarantee program in Thailand

    OpenAIRE

    Wagener, Andreas; Zenker, Juliane

    2015-01-01

    From 2009 to 2011, the Thai government implemented an income guarantee program for rice, tapioca and maize farmers. Essentially, this program added a non-negative but stochastic component to the incomes of registered farmers. We evaluate the impact of the program on risk attitudes and investment behavior of small-scale rice farmers in relatively poor North-eastern Thailand. To control for self-selection into the scheme, we use propensity score matching. We find that that participation in the ...

  20. The UK Future Jobs Fund: Labour’s adoption of the job guarantee principle

    OpenAIRE

    Ali, Tanweer

    2011-01-01

    This paper examines the development of employment policy in the United Kingdom leading to the creation of the Young Person’s Guarantee and its main component, the Future Jobs Fund. Past public-sector direct employment schemes, including those associated with the workfare model, had been discredited as ineffective across the OECD. In numerous countries, however, newer job creation schemes were implemented from the 1990s, aimed at addressing some of the shortcomings of earlier projects, and uti...

  1. Justifying a revised voting rights act: The Guarantee Clause and the problem of minority rule

    OpenAIRE

    Chin, GJ

    2014-01-01

    In Shelby County v. Holder, the Supreme Court invalidated Section 4 of the Voting Rights Act of 1965, which required certain jurisdictions with histories of discrimination to "preclear" changes to their voting practices under Section 5 before those changes could become effective. This Article proposes that Congress ground its responsive voting rights legislation in the Constitution's Guarantee Clause, in addition to the Fourteenth and Fifteenth Amendments. The Court has made clear that the Gu...

  2. Mutual Loan-Guarantee Societies in Monopolistic Credit Markets with Adverse Selection

    OpenAIRE

    Giovanni Busetta; Alberto Zazzaro

    2009-01-01

    In many countries, Mutual Loan-Guarantee Societies (MLGSs) are assuming ever-increasing importance for small business lending. In this paper we provide a theory to rationalise the raison d'^etre of MLGSs. The basic intuition is that the foundation for MLGSs lies in the inefficiencies created by adverse selection, when borrowers do not have enough collateralisable wealth to satisfy collateral requirements and induce self-selecting contracts. In this setting, we view MLGSs as a wealth-pooling m...

  3. Minimum Guaranteed Payments and Costly Cancellation Rights: A Stopping Game Perspective

    OpenAIRE

    Luis H. R. Alvarez E.

    2006-01-01

    We consider the valuation and optimal exercise policy of a δ- penalty minimum guaranteed payment option in the case where the value of the underlying dividend-paying asset follows a linear diffusion. We characterize both the value and optimal exercise policy of the considered game option explicitly and demonstrate that increased volatility increases the value of the option and postpones exercise by expanding the continuation region where exercising is suboptimal. An interesting and natural im...

  4. Comonotonic Approximations of Risk Measures for Variable Annuity Guaranteed Benefits with Dynamic Policyholder Behavior

    OpenAIRE

    Feng, Runhuan; Jing, Xiaochen; Dhaene, Jan

    2015-01-01

    The computation of various risk metrics is essential to the quantitative risk management of variable annuity guaranteed benefits. The current market practice of Monte Carlo simulation often requires intensive computations, which can be very costly for insurance companies to implement and take so much time that they cannot obtain information and take actions in a timely manner. In an attempt to find low-cost and efficient alternatives, we explore the techniques of comonotonic bounds to produce...

  5. An Evaluation of Financial Inclusion through Mahatma Gandhi National Rural Employment Guarantee Programme

    OpenAIRE

    Kundu, Amit

    2013-01-01

    The government of India had initiated bank payments of wages under Mahatma Gandhi National Rural Employment Guarantee Programme (MGNREGP). This initiative is undoubtedly a big financial inclusion drive particularly among the rural poor of India. This paper has tried to evaluate this financial inclusion drive through MGNREGP in Mandir Bazar block of South 24 Parganas district of West Bengal on the basis of field survey. The picture came out from field research is very encouraging. It is establ...

  6. A convex pseudo-likelihood framework for high dimensional partial correlation estimation with convergence guarantees

    OpenAIRE

    Khare, Kshitij; Oh, Sang-Yun; Rajaratnam, Bala

    2013-01-01

    Sparse high dimensional graphical model selection is a topic of much interest in modern day statistics. A popular approach is to apply l1-penalties to either (1) parametric likelihoods, or, (2) regularized regression/pseudo-likelihoods, with the latter having the distinct advantage that they do not explicitly assume Gaussianity. As none of the popular methods proposed for solving pseudo-likelihood based objective functions have provable convergence guarantees, it is not clear if corresponding...

  7. "The Social Enterprise Model for a Job Guarantee in the United States"

    OpenAIRE

    Pavlina R. Tcherneva

    2014-01-01

    The job guarantee is a proposal that provides greater macroeconomic stability and secures a fundamental human right. Despite the economic and moral merits of this policy, often the program is rejected because of concerns about its administration. How would the program be implemented? Who will create the jobs? Can work be found for every unemployed individual who wishes to work? This policy note addresses these concerns by elaborating on a proposal for the United States that would run the job ...

  8. Limit of Payback Period Applicability at Creation of Guaranteed Redemption Prices

    OpenAIRE

    Josef Valach

    2013-01-01

    Payback period is not suitability criterion for evaluation great part of investment projects and also for creation guaranteed redemption price of energy. It ignores time factor and also cash flows after the cut-off date. For projects with greater internal rates of return and with longer economic lives it can be proved that evaluation by payback period and by internal rate of return is significantly approximate. Interest payments from long debt capital should not be included in project cash fl...

  9. A New Performance Guarantee for Orthogonal Matching Pursuit Using Mutual Coherence

    OpenAIRE

    Emadi, Mohammad; Miandji, Ehsan; Unger, Jonas; Afshari, Ehsan

    2016-01-01

    In this paper we present a new coherence-based performance guarantee for the Orthogonal Matching Pursuit (OMP) algorithm. An upper bound for the probability of correctly identifying the support of a sparse signal with additive white Gaussian noise is derived. Compared to previous work, the new bound takes into account the signal parameters such as dynamic range, noise variance, and sparsity. Numerical simulations show significant improvements over previous work.

  10. Implicit state guarantees exacerbate problem: Separated banking system alone not a solution

    OpenAIRE

    Klaus, Benjamin; Schäfer, Dorothea

    2013-01-01

    Many banks are now too big, complex, and closely interconnected to be liquidated. When they run into difficulties, they threaten the entire financial system of their economic area. Five years of financial crisis have not alleviated but exacerbated this problem. The cost of stabilizing banks is enormous, posing serious challenges to the states affected. In addition, such state guarantees create dangerously false incentives: they encourage managers and investors to engage into high risk-taking,...

  11. Performance Analysis and Coherent Guaranteed Cost Control for Uncertain Quantum Systems

    OpenAIRE

    Xiang, Chengdi; Petersen, Ian R.; Dong, Daoyi

    2014-01-01

    This paper presents several results on performance analysis for a class of uncertain linear quantum systems subject to either quadratic or non-quadratic perturbations in the system Hamiltonian. Also, coherent guaranteed cost controllers are designed for the uncertain quantum systems to achieve improved control performance. The coherent controller is realized by adding a control Hamiltonian to the quantum system and its performance is demonstrated by an example.

  12. Do government loan guarantee instruments make sense? : evaluating effectiveness of the tranched cover fund

    OpenAIRE

    Viggiano, Claudio

    2016-01-01

    Small and Medium Enterprises (SMEs) have a fundamental role in advanced economies in generating employment and in fostering innovation, but they often struggle with lack of financing resources due to the presence of information asymmetries. Loan Guarantee Instruments are the most adopted schemes to mitigate market imperfections, allowing banks to shift some risk on the guarantor, thus leading to a less restrictive lending behavior. The overall aim of this thesis is to examine whether the T...

  13. Guaranteed Cost Fault-Tolerant Control for Networked Control Systems with Sensor Faults

    OpenAIRE

    Qixin Zhu; Kaihong Lu; Guangming Xie; Yonghong Zhu

    2015-01-01

    For the large scale and complicated structure of networked control systems, time-varying sensor faults could inevitably occur when the system works in a poor environment. Guaranteed cost fault-tolerant controller for the new networked control systems with time-varying sensor faults is designed in this paper. Based on time delay of the network transmission environment, the networked control systems with sensor faults are modeled as a discrete-time system with uncertain parameters. And the mode...

  14. Investigations to guarantee the adminissible power densities in the core of pressurized power reactors

    International Nuclear Information System (INIS)

    Employing the computer code Garlic for simulation of the three-dimensional core behaviour and the control and limitation systems, analyses have been made in order to assess the effectiveness of the limiting actions to guarantee the admissible power densities in the core. Examples from normal and disturbed operating conditions have shown that the limitation systems are effective to observe the admissible operating region following the guidelines of the TUeV-Leitstelle Kerntechnik in all cases analyzed. (orig.)

  15. Molecular Studies on Methicillin-Resistant Staphylococcus aureus

    International Nuclear Information System (INIS)

    The present study of the MecA gene in our clinical isolates has been detected and verified by antibiotic disc diffusion test and nested Polymerase Chain Reaction (PCR). Part of the product of the second PCR was also sequenced. The results indicated 97.7% similarity between the sequences of the mecA gene isolated from an Egyptian Staphylococcus aureus strain and that compared from Staphylococcus aureus strain no. GI46628 cited at the European Molecular Biology Laboratory (EMBL) database under accession number Y00688 in the region from nucleotide 467 to 875. The effect of gamma-radiation on these isolates was observed by plotting the dose survival curves of these isolates and determining their D10 values. Their D10 values were found to be ranged from 0.44 to 0.66 kGy. Antibiotic sensitivity tests were also carried out after exposure of Oxacillin-susceptible isolate to sub -lethal doses of γ-radiation.Results indicated that Staphylococcus aureus isolates which were sensitive to oxacillin discs were found by PCR to harbor the mecA gene in their genomes. Also, exposure of a sensitive isolate to sublethal doses of gamma radiation led to the emergence of a oxacillin-resistant variant which could be a serious problem in case of using sub-lethal doses of radiation for the sterilization of medical products

  16. Novel antibiotics for the treatment of Staphylococcus aureus.

    Science.gov (United States)

    Ohlsen, Knut

    2009-11-01

    Staphylococcus aureus is a leading cause of nosocomial and community-acquired infection associated with significant morbidity and mortality. Antibiotic treatment of infections owing to S. aureus have become increasingly challenging as the pathogen has acquired a broad spectrum of antibiotic resistance mechanisms. In particular, emergence and spread of methicillin-resistant S. aureus (MRSA) progressed to a global health threat. The glycopeptides antibiotics vancomycin and teicoplanin have remained as the drugs of last resort for more than 20 years. Fortunately, in addition to the glycopeptides, several novel antibiotics including linezolid, daptomycin, tigecycline, quinupristin/dalfopristin and ceftobiprole acting against MRSA have been recently introduced into clinical practice broadening therapeutic options. Although the arsenal of antistaphylococcal drugs has filled up in recent years, the rate of MRSA infection continues to be high in most countries. This demands an ongoing search for new antibacterials and lead compounds as well as development of alternative therapies and faster diagnostics to ensure effective anti-staphylococcal therapy in the future. PMID:22112259

  17. Synthetic peptide inhibitors of DNA replication in Staphylococcus aureus

    DEFF Research Database (Denmark)

    Løbner-Olesen, Anders; Kjelstrup, Susanne

    of clinically important pathogens and is essential for bacterial proliferation. The bacterial replication apparatus fulfill the requirements for a good drug target. The replisome of S. aureus consists of 5 different subunits (2, PolC2, 4, δ and δ`) who’s organization depends on multiple protein......-protein interactions. Centrally in the replisome is the -clamp where to multiple proteins binds through a conserved motif. We have identified the protein-protein interactions in the replisome of S. aureus by use of a bacterial two-hybrid system. A reverse bacterial two-hybrid system (R-BTH) based on Pyr......N (), DnaB and DnaX (). Three peptides identified as inhibitors of DnaN have been purified. Two of these peptides inhibited growth as well as DNA replication in S. aureus. The minimal inhibitory concentration (MIC) of the peptides was approximately 50 g/ml. Overexpression of DnaN reduced the inhibitory...

  18. Purification and crystallization of RNase HIII from Staphylococcus aureus

    International Nuclear Information System (INIS)

    The purification, crystallization and preliminary X-ray diffraction analysis of RNase HIII from S. aureus is presented. Crystals that diffracted to 2.6 Å resolution in space group P212121 were only obtained after removal of the hexahistidine tag. As part of collaborative efforts to characterize virulence factors from Staphylococcus aureus, methods for the large-scale recombinant production of RNase HIII from S. aureus subspecies MRSA252 (Sa-RNase HIII) have been developed. RNase HIII-type ribonucleases are poorly characterized members of the RNase H group of endonucleases which hydrolyze RNA from RNA/DNA hybrids and are thought to be involved in DNA replication and repair. They are characterized by N-terminal extensions of unknown function that do not share sequence homology with the N-terminal extensions of bacterial RNases HI and RNases HII. Sa-RNase HIII was crystallized in the orthorhombic space group P212121, with unit-cell parameters a = 48.9, b = 74.2, c = 127.5 Å, and diffracted to 2.6 Å resolution

  19. Staphylococcus aureus Regulatory RNAs as Potential Biomarkers for Bloodstream Infections.

    Science.gov (United States)

    Bordeau, Valérie; Cady, Anne; Revest, Matthieu; Rostan, Octavie; Sassi, Mohamed; Tattevin, Pierre; Donnio, Pierre-Yves; Felden, Brice

    2016-09-01

    Staphylococcus aureus is a commensal bacterium and pathogen. Identifying biomarkers for the transition from colonization to disease caused by this organism would be useful. Several S. aureus small RNAs (sRNAs) regulate virulence. We investigated presence and expression of 8 sRNAs in 83 S. aureus strains from 42 patients with sepsis or septic shock and 41 asymptomatic colonized carriers. Small pathogenicity island sRNAs sprB and sprC were clade specific. Six sRNAs had variable expression not correlated with clinical status. Expression of RNAIII was lower in strains from septic shock patients than in strains from colonized patients. When RNAIII was associated with expression of sprD, colonizing strains could be discriminated from strains in patients with bloodstream infections, including patients with sepsis and septic shock. Isolates associated with colonization might have sRNAs with target expression different from those of disease isolates. Monitoring expression of RNAIII and sprD could help determine severity of bloodstream infections. PMID:27224202

  20. Biochemical characters and antibiotic susceptibility of Staphylococcus aureus isolates

    Institute of Scientific and Technical Information of China (English)

    Subhankari Prasad Chakraborty; Santanu Kar Mahapatra; Somenath Roy

    2011-01-01

    Objective: To observe the biochemical characters and antibiotic susceptibility of isolated Staphylococcus aureus (S. auerus) strains against some conventional and traditional antibiotics.Methods:Bacterial culture was done in Mueller-Hinton broth at 37 ℃. Characters of these strains were determined by traditional biochemical tests such as hydrolysis test of gelatin, urea, galactose, starch and protein, and fermentation of lactose and sucrose. Antibiotic susceptibility were carried out by minimum inhibitory concentration test, minium bactericidal concentration test, disc agar diffusion test and brain heart infusion oxacillin screening agar. Results: From this study, it was observed that 100% S. aureus isolates showed positive results in gelatin, urea and galactose hydrolysis test, 50% isolates were positive in starch hydrolysis test, 35% in protein hydrolysis test, 100% isolates in lactose fermenting test, but no isolate was positive in sucrose fermenting test. Antibiotic susceptibility testing suggested that 20% of isolates were resistant to kanamycin and 46.67% were resistant to oxacillin. Conclusions: These findings show that all these isolates have gelatin, urea, galactose hydrolysis and lactose fermenting activity. 20% of these isolates were resistant to kanamycin and 46.67% were resistant to oxacillin. Thirty post operative pathogenic isolated S. aureus strains were used in this study.

  1. FP/FIFO scheduling: coexistence of deterministic and probabilistic QoS guarantees

    Directory of Open Access Journals (Sweden)

    Pascale Minet

    2007-01-01

    Full Text Available In this paper, we focus on applications having quantitative QoS (Quality of Service requirements on their end-to-end response time (or jitter. We propose a solution allowing the coexistence of two types of quantitative QoS garantees, deterministic and probabilistic, while providing a high resource utilization. Our solution combines the advantages of the deterministic approach and the probabilistic one. The deterministic approach is based on a worst case analysis. The probabilistic approach uses a mathematical model to obtain the probability that the response time exceeds a given value. We assume that flows are scheduled according to non-preemptive FP/FIFO. The packet with the highest fixed priority is scheduled first. If two packets share the same priority, the packet arrived first is scheduled first. We make no particular assumption concerning the flow priority and the nature of the QoS guarantee requested by the flow. An admission control derived from these results is then proposed, allowing each flow to receive a quantitative QoS guarantee adapted to its QoS requirements. An example illustrates the merits of the coexistence of deterministic and probabilistic QoS guarantees.

  2. Guarantee Funds of Credits in Romania - Necessity, Features and Networking - II

    Directory of Open Access Journals (Sweden)

    Georgeta Dragomir

    2011-12-01

    Full Text Available In the current context, marked by financial crisis, economic recession, lack of liquidity andlow capitalization, the access tofinancing is more difficult, mainly due to the lack, insufficiency orlimited availability of guarantees. Guarantee funds represent an innovative solution, proven by theEuropean economies and recently launched in Romania. Taking over the international experience inorder to facilitate the SMEs’ access to financial resources has become a necessity in Romania, whichwas reflected by the creation of three specialized financial institutions in granting guarantees domain.These are bodies with private or publiccapital, which relate, based on agreements with commercialbanks. After a period of exposure to major risks from the lack of regulatory activity, the guaranteefunds, along with other non-banking financial institutions are absolutely governed by rules oftheNRB, according to the banking law and special laws. The analysis in this paper is based on the studyof several official documents, reports of specialized bodies, statistics and examples of good practice,summarized and interpreted in through comparison and developments. The aim is to identify anumber of issues that may lead to sensitize the responsible factors-public authorities, creditinstitutions, and specialists-to come up with new practical solutions that would stimulate thefinancing processof small entrepreneurs.

  3. Mahanaxar: quality of service guarantees in high-bandwidth, real-time streaming data storage

    Energy Technology Data Exchange (ETDEWEB)

    Bigelow, David [Los Alamos National Laboratory; Bent, John [Los Alamos National Laboratory; Chen, Hsing-Bung [Los Alamos National Laboratory; Brandt, Scott [UCSC

    2010-04-05

    Large radio telescopes, cyber-security systems monitoring real-time network traffic, and others have specialized data storage needs: guaranteed capture of an ultra-high-bandwidth data stream, retention of the data long enough to determine what is 'interesting,' retention of interesting data indefinitely, and concurrent read/write access to determine what data is interesting, without interrupting the ongoing capture of incoming data. Mahanaxar addresses this problem. Mahanaxar guarantees streaming real-time data capture at (nearly) the full rate of the raw device, allows concurrent read and write access to the device on a best-effort basis without interrupting the data capture, and retains data as long as possible given the available storage. It has built in mechanisms for reliability and indexing, can scale to meet arbitrary bandwidth requirements, and handles both small and large data elements equally well. Results from our prototype implementation shows that Mahanaxar provides both better guarantees and better performance than traditional file systems.

  4. Guaranteeing Isochronous Control of Networked Motion Control Systems Using Phase Offset Adjustment

    Directory of Open Access Journals (Sweden)

    Ikhwan Kim

    2015-06-01

    Full Text Available Guaranteeing isochronous transfer of control commands is an essential function for networked motion control systems. The adoption of real-time Ethernet (RTE technologies may be profitable in guaranteeing deterministic transfer of control messages. However, unpredictable behavior of software in the motion controller often results in unexpectedly large deviation in control message transmission intervals, and thus leads to imprecise motion. This paper presents a simple and efficient heuristic to guarantee the end-to-end isochronous control with very small jitter. The key idea of our approach is to adjust the phase offset of control message transmission time in the motion controller by investigating the behavior of motion control task. In realizing the idea, we performed a pre-runtime analysis to determine a safe and reliable phase offset and applied the phase offset to the runtime code of motion controller by customizing an open-source based integrated development environment (IDE. We also constructed an EtherCAT-based motion control system testbed and performed extensive experiments on the testbed to verify the effectiveness of our approach. The experimental results show that our heuristic is highly effective even for low-end embedded controller implemented in open-source software components under various configurations of control period and the number of motor drives.

  5. Guaranteeing QoS of media-based applications in virtualized environment

    Science.gov (United States)

    Zhou, Like; Wu, Song; Shi, Xuanhua; Jin, Hai; Zhou, Jiangfu

    2013-12-01

    With the rapid development of web technology and smart phone, multimedia contents spread all over the Internet. The prevalence of virtualization technology enables multimedia service providers to run media servers in virtualized servers or rented virtual machines (VMs) in a cloud environment. Although server consolidation using virtualization can substantially increase the efficient use of server resources, it introduces resources competition among VMs running different applications. Recently, hypervisors do not make any Quality of Service (QoS) guarantee for media-based applications if they are consolidated with other network-intensive applications, which leads to significant performance degradation. For example, Xen only offers a static method to allocate network bandwidth. In this paper, we find that the performance of media-based applications running in VMs degrades seriously when they are consolidated with other VMs running network-intensive applications and argues that dynamic network bandwidth allocation is essential to guarantee the QoS of media-based applications. Then, we present a dynamic network bandwidth allocation system in virtualized environment, which allocates network bandwidth dynamically and effectively, and does not interrupt running services in VMs. The experiments show that our system can not only guarantee the QoS of media-based applications well but also maximize the system's the overall performance while ensuring the QoS of media-based applications.

  6. Role of GapC in the pathogenesis of Staphylococcus aureus.

    Science.gov (United States)

    Kerro-Dego, Oudessa; Prysliak, Tracy; Perez-Casal, Jose; Potter, Andrew A

    2012-05-01

    Staphylococcus aureus is recognized worldwide as a major pathogen causing clinical or subclinical intramammary infections in lactating cows, sheep and goats. S. aureus produces a wide arsenal of cell surface and extracellular proteins involved in virulence. Among these are two conserved proteins with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity named glyceraldehyde-3-phosphate dehydrogenase-B (GapB) and -C (GapC). In this study, we used the S. aureus wild type strain RN6390 and its isogenic gapC mutant H330 in in vitro and in vivo studies and determined that the S. aureus GapC protein plays a role on adherence to and internalization into bovine mammary epithelial (MAC-T) cells. In addition, we found that S. aureus H330 did not caused mastitis after an experimental infection of ovine mammary glands. Together, these results show that GapC is important in the pathogenesis of S. aureus mastitis. PMID:22176759

  7. Neonatal colonization with Staphylococcus aureus is not associated with development of atopic dermatitis

    DEFF Research Database (Denmark)

    Skov, L; Halkjaer, L B; Agner, T;

    2009-01-01

    BACKGROUND: Staphylococcus aureus in atopic skin has been associated with exacerbation of eczema. Objectives To investigate a possible association between neonatal colonization with S. aureus and the risk of atopic dermatitis (AD) during the first 3 years of life. MATERIALS AND METHODS: The study...... monitored prospectively. RESULTS: Of the neonates, 5.3% had positive swabs for S. aureus cultured from the vestibulum nasi (51.3%) and/or the perineum (11.3%). Forty-two per cent developed AD, but without association between colonization with S. aureus at 1 month of age and risk of developing AD at 3 years...... of age. There was a 70% concordance for S. aureus carriage between neonates and parents. At 1 year of age 11.3% children had swabs positive for S. aureus. Fourteen per cent of children tested at the 1-year visit developed AD after the visit but before 3 years of age, but again, there was no association...

  8. The growth of Staphylococcus aureus and Escherichia coli in low-direct current electric fields

    Institute of Scientific and Technical Information of China (English)

    Dunya Zituni; Heidi Schu tt-Gerowitt; Marion Kopp; Martin Kro nke; Klaus Addicks; Christian Hoffmann; Martin Hellmich; Franz Faber; Wilhelm Niedermeier

    2014-01-01

    Electrical potentials up to 800 mV can be observed between different metallic dental restorations. These potentials produce fields in the mouth that may interfere with microbial communities. The present study focuses on the impact of different electric field strengths (EFS) on the growth of Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922) in vitro. Cultures of S. aureus and E. coli in fluid and gel medium were exposed to different EFS. Effects were determined by calculation of viable counts and measurement of inhibition zones. In gel medium, anodic inhibition zones for S. aureus were larger than those for E. coli at all field strength levels. In fluid medium, the maximum decrease in the viable count of S. aureus cells was at 10 V?m21. Field-treated S. aureus cells presented ruptured cell walls and disintegrated cytoplasm. Conclusively, S. aureus is more sensitive to increasing electric field strength than E. coli.

  9. One-year mortality in coagulase-negative Staphylococcus and Staphylococcus aureus infective endocarditis

    DEFF Research Database (Denmark)

    Rasmussen, Rasmus V; Snygg-Martin, Ulrika; Olaison, Lars;

    2009-01-01

    The aim of this study was to investigate in-hospital mortality and 12-month mortality in patients with coagulase-negative Staphylococcus (CoNS) compared to Staphylococcus aureus (S. aureus) infective endocarditis (IE). We used a prospective cohort study of 66 consecutive CoNS and 170 S. aureus IE...... patients, collected at 2 tertiary university hospitals in Copenhagen (Denmark) and at 1 tertiary university hospital in Gothenburg (Sweden). Median (range) C-reactive protein at admission was higher in patients with S. aureus IE (150 mg/l (1-521) vs 94 mg/l (6-303); p...% of patients with S. aureus IE (p =0.05). In conclusion, CoNS IE was associated with a long diagnostic delay and high in-hospital mortality, whereas post-discharge prognosis was better in this group of patients compared to patients with IE due to S. aureus....

  10. Use of mupirocin-chlorhexidine treatment to prevent Staphylococcus aureus surgical-site infections.

    Science.gov (United States)

    Bertrand, X; Slekovec, C; Talon, D

    2010-05-01

    Evaluation of: Bode LGM, Kluytmans JAJW, Wertheim HFL et al.: Preventing surgical-site infections in nasal carriers of Staphylococcus aureus. N. Engl. J. Med. 362, 9-17 (2010). Staphylococcus aureus is the main pathogen responsible for surgical-site infections and nasal carriage is a major risk factor for subsequent infection with this bacteria. Mupirocin is considered to be the topical antibacterial agent of choice for eradication of nasal S. aureus. The paper by Bode et al. provides strong evidence that the combination of a rapid identification of a S. aureus nasal carrier, mupirocin nasal ointment and chlorhexidine gluconate soap, significantly reduces the rate of S. aureus surgical-site infection by nearly 60%. In conclusion, mupirocin nasal ointment use in S. aureus carriers before surgery has numerous advantages with few side effects. PMID:20441543

  11. Pattern differentiation in co-culture biofilms formed by Staphylococcus aureus and Pseudomonas aeruginosa

    DEFF Research Database (Denmark)

    Yang, Liang; Liu, Yang; Markussen, Trine;

    2011-01-01

    -culture biofilms. By growing co-culture biofilms of S. aureus with P. aeruginosa mutants in a flow-chamber system and observing them using confocal laser scanning microscopy, we show that wild-type P. aeruginosa PAO1 facilitates S. aureus microcolony formation. In contrast, P. aeruginosa mucA and rpoN mutants do...... not facilitate S. aureus microcolony formation and tend to outcompete S. aureus in co-culture biofilms. Further investigations reveal that extracellular DNA (eDNA) plays an important role in S. aureus microcolony formation and that P. aeruginosa type IV pili are required for this process, probably through...... their ability to bind to eDNA. Furthermore, P. aeruginosa is able to protect S. aureus against Dictyostelium discoideum phagocytosis in co-culture biofilms....

  12. The dynamics of Staphylococcus aureus intramammary infection in nine Danish dairy herds

    DEFF Research Database (Denmark)

    Larsen, H. D.; Sloth, K. H.; Elsberg, C.;

    2000-01-01

    The aim of the present study was to examine the diversity of Staphylococcus aureus isolates from bovine intramammary infections (IMI) in nine dairy herds, and compare these with isolates from other sites on the cows by phage- and ribotyping. Whether colonisation of milkers with S. aureus could...... be a source of infection for bovine IMI was investigated. In addition, 100 epidemiologically unrelated S. aureus isolates from asymptomatic human carriers were also phage- and ribotyped to compare the human and bovine reservoir of S. aureus in Denmark. A total of 625 S. aureus isolates from bovine IMI, bovine......, there was a close correspondence between ribo- and phage types of S. aureus isolated from bovine intramammmary infections and skin lesions. Isolates from milking personnel, however, were not identical to any of the predominant intramammary strains. Furthermore, several of the isolates from milking personnel showed...

  13. Antimicrobial susceptibility of Staphylococcus aureus and characterization of methicillin-resistant Staphylococcus aureus isolated from bovine mastitis in Korea.

    Science.gov (United States)

    Nam, Hyang-Mi; Lee, Ae-Li; Jung, Suk-Chan; Kim, Mal-Nam; Jang, Geum-Chan; Wee, Sung-Hwan; Lim, Suk-Kyung

    2011-02-01

    A total of 402 Staphylococcus aureus isolates from bovine mastitis milk collected during 2003-2009 in Korea were tested for susceptibility to 20 antimicrobial agents. All S. aureus isolates were susceptible to 11 of 20 antimicrobials tested; no resistance was observed against pirlimycin, telithromycin, novobiocin, penicillin/novobiocin, quinupristin/dalfopristin, clindamycin, rifampin, ciprofloxacin, trimethprim/sulfamethoxazol, vancomycin, and linezolid. Over 66% of the S. aureus isolates were resistant to penicillin. Resistance was also seen for gentamicin (11.9%), erythromycin (7.7%), methicillin (oxacillin and cefoxitin, 6.2%), and tetracycline (4.2%). No noticeable change was observed in penicillin, gentamicin, and erythromycin resistance over the 7-year period. Tetracycline resistance appeared to decrease consistently, whereas methicillin resistance was observed from 2005. About 2.7% (11/402) were resistant to three or more antimicrobials. Genotyping of the 17 methicillin-resistant S. aureus (MRSA) isolated from each cow revealed two staphylococcal cassette chromosome mec (SCCmec) types (IV and IVa), three spa types (t286, t324, and untypable), and two sequence types (ST1 and ST72). Eleven of 17 (64.7%) MRSA strains belonged to SCCmec IVa-t324-ST72. The rest of strains belonged to SCCmec IVa-t286-ST1 (n = 3) and SCCmec IV-untypable-ST72 (n = 3). None of the MRSA carried the Panton-Valentine leukocidin gene. These characteristics are the same as those found in community-acquired (CA) MRSA strains prevalent in humans in Korea. Three pulsed-field gel electrophoresis types (A-C) were observed among the 17 MRSA strains examined, and 14 strains belonged to the same pulsed-field gel electrophoresis pattern regardless of their geographical origin and year of isolation. The results of this study provide evidence of CA-MRSA infection in dairy cattle for the first time in Korea. PMID:21034263

  14. Surgimiento y diseminación de Staphylococcus aureus meticilinorresistente Staphylococcus aureus methicillin-resistant: emergence and dissemination

    OpenAIRE

    Maria Elena Velázquez-Meza

    2005-01-01

    Las infecciones nosocomiales ocasionadas por cepas de Staphylococcus aureus meticilinorresistentes (SAMR) son un problema de salud importante en todo el mundo. Este microorganismo produce una gran variedad de infecciones incluyendo osteomielitis, endocarditis invasora, artritis séptica y septicemia. La multirresistencia es un factor que influye en la persistencia de los SAMR dentro del ámbito hospitalario. La introducción de técnicas de tipificación molecular dentro de las investigaciones epi...

  15. VARIABILITY OF PHENOTYPIC, PROTEOMIC AND TRANSCRIPTOMIC EXPRESSION OF STAPHYLOCOCCUS AUREUS SURFACE ADHESINS

    OpenAIRE

    Ythier M.

    2012-01-01

    Staphylococcus aureus is a highly successful pathogen responsible of a wide variety of diseases, from minor skin infection to life-threatening sepsis or infective endocarditis, as well as food poisoning and toxic shock syndrome. This heterogeneity of infections and the ability of S. aureus to develop antibiotic-resistance to virtually any available drugs reflect its extraordinary capacity to adapt and survive in a great variety of environments. The pathogenesis of S. aureus infection involves...

  16. Glycolytic Dependency of High-Level Nitric Oxide Resistance and Virulence in Staphylococcus aureus

    OpenAIRE

    Vitko, Nicholas P.; Spahich, Nicole A.; Richardson, Anthony R.

    2015-01-01

    ABSTRACT Staphylococcus aureus is a prolific human pathogen capable of causing severe invasive disease with a myriad of presentations. The ability of S. aureus to cause infection is strongly linked with its capacity to overcome the effects of innate immunity, whether by directly killing immune cells or expressing factors that diminish the impact of immune effectors. One such scenario is the induction of lactic acid fermentation by S. aureus in response to host nitric oxide (NO·). This ferment...

  17. Exposure and risk assessment of Staphylococcus aureus in food chain in Slovakia

    OpenAIRE

    Ľubomír Valík; Alžbeta Medveďová

    2013-01-01

    Foods can been contaminated by S. aureus usually as a result of unhygienic behaviour of staff or improper conducted technological procedures. Compared to saprophytic bacteria, S. aureus does not possess significant competitive properties; however its epidemiological potential consists in multiplication to density higher than 106 cfu/g and formation of heat-resistant enterotoxins by which it causes food poisoning. Based on the data in 2001, the risk was characterized as follows: S. aureus is ...

  18. Subinhibitory concentrations of perilla oil affect the expression of secreted virulence factor genes in Staphylococcus aureus.

    Directory of Open Access Journals (Sweden)

    Jiazhang Qiu

    Full Text Available BACKGROUND: The pathogenicity of staphylococcus aureus is dependent largely upon its ability to secrete a number of virulence factors, therefore, anti-virulence strategy to combat S. aureus-mediated infections is now gaining great interest. It is widely recognized that some plant essential oils could affect the production of staphylococcal exotoxins when used at subinhibitory concentrations. Perilla [Perilla frutescens (L. Britton], a natural medicine found in eastern Asia, is primarily used as both a medicinal and culinary herb. Its essential oil (perilla oil has been previously demonstrated to be active against S. aureus. However, there are no data on the influence of perilla oil on the production of S. aureus exotoxins. METHODOLOGY/PRINCIPAL FINDINGS: A broth microdilution method was used to determine the minimum inhibitory concentrations (MICs of perilla oil against S. aureus strains. Hemolysis, tumour necrosis factor (TNF release, Western blot, and real-time RT-PCR assays were performed to evaluate the effects of subinhibitory concentrations of perilla oil on exotoxins production in S. aureus. The data presented here show that perilla oil dose-dependently decreased the production of α-toxin, enterotoxins A and B (the major staphylococcal enterotoxins, and toxic shock syndrome toxin 1 (TSST-1 in both methicillin-sensitive S. aureus (MSSA and methicillin-resistant S. aureus (MRSA. CONCLUSIONS/SIGNIFICANCE: The production of α-toxin, SEA, SEB, and TSST-1 in S. aureus was decreased by perilla oil. These data suggest that perilla oil may be useful for the treatment of S. aureus infections when used in combination with β-lactam antibiotics, which can increase exotoxins production by S. aureus at subinhibitory concentrations. Furthermore, perilla oil could be rationally applied in food systems as a novel food preservative both to inhibit the growth of S. aureus and to repress the production of exotoxins, particularly staphylococcal enterotoxins.

  19. Methicillin-resistant Staphylococcus aureus colonization among pediatric health care workers from different outpatient settings

    OpenAIRE

    Immergluck, Lilly Cheng; Satola, Sarah W.; Jain, Shabnam; Courtney, McCracken; Watson, J. Reneé; Chan, Trisha; Traci, Leong; Gottlieb, Edward; Jerris, Robert C

    2013-01-01

    Staphylococcus aureus colonization rates in pediatric health care workers from different types of outpatient settings were determined from December 2008 through May 2010. Colonization rates for Staphylococcus aureus and, specifically, methicillin-resistant Staphylococcus aureus (MRSA) rates were similar to the rates that have been reported for the general population. The predominant MRSA pulsed-field gel electrophoresis type associated with colonization in these health care workers is not MRS...

  20. IsdB-dependent Hemoglobin Binding Is Required for Acquisition of Heme by Staphylococcus aureus

    OpenAIRE

    Pishchany, Gleb; Sheldon, Jessica R.; Dickson, Claire F.; Alam, Md Tauqeer; Read, Timothy D.; Gell, David A; Heinrichs, David E.; Skaar, Eric P.

    2013-01-01

    Staphylococcus aureus is a Gram-positive pathogen responsible for tremendous morbidity and mortality. As with most bacteria, S. aureus requires iron to cause disease, and it can acquire iron from host hemoglobin. The current model for staphylococcal hemoglobin-iron acquisition proposes that S. aureus binds hemoglobin through the surface-exposed hemoglobin receptor IsdB. IsdB removes heme from bound hemoglobin and transfers this cofactor to other proteins of the Isd system, which import and de...