Comparative study of adipose-derived stem cells and bone marrow-derived stem cells in similar microenvironmental conditions

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Guneta, Vipra [Division of Materials Technology, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798 (Singapore); Tan, Nguan Soon [School of Biological Science, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551 (Singapore); KK Research Centre, KK Women' s and Children Hospital, 100 Bukit Timah Road, Singapore 229899 (Singapore); Institute of Molecular and Cell Biology, Agency for Science Technology & Research - A*STAR, 61 Biopolis Drive, Proteos, Singapore 138673 (Singapore); Chan, Soon Kiat Jeremy [School of Biological Science, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551 (Singapore); Tanavde, Vivek [Bioinformatics Institute, Agency for Science Technology & Research - A*STAR, 30 Biopolis Street, Matrix, Singapore 138671 (Singapore); Lim, Thiam Chye [Division of Plastic, Reconstructive and Aesthetic Surgery, Department of Surgery, National University Hospital (NUH) and National University of Singapore (NUS), Kent Ridge Wing, Singapore 119074 (Singapore); Wong, Thien Chong Marcus [Plastic, Reconstructive and Aesthetic Surgery Section, Tan Tock Seng Hospital (TTSH), 11, Jalan Tan Tock Seng, Singapore 308433 (Singapore); Choong, Cleo, E-mail: cleochoong@ntu.edu.sg [Division of Materials Technology, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798 (Singapore); KK Research Centre, KK Women' s and Children Hospital, 100 Bukit Timah Road, Singapore 229899 (Singapore)

2016-11-01

Mesenchymal stem cells (MSCs), which were first isolated from the bone marrow, are now being extracted from various other tissues in the body, including the adipose tissue. The current study presents systematic evidence of how the adipose tissue-derived stem cells (ASCs) and bone marrow-derived mesenchymal stem cells (Bm-MSCs) behave when cultured in specific pro-adipogenic microenvironments. The cells were first characterized and identified as MSCs in terms of their morphology, phenotypic expression, self-renewal capabilities and multi-lineage potential. Subsequently, the proliferation and gene expression profiles of the cell populations cultured on two-dimensional (2D) adipose tissue extracellular matrix (ECM)-coated tissue culture plastic (TCP) and in three-dimensional (3D) AlgiMatrix® microenvironments were analyzed. Overall, it was found that adipogenesis was triggered in both cell populations due to the presence of adipose tissue ECM. However, in 3D microenvironments, ASCs and Bm-MSCs were predisposed to the adipogenic and osteogenic lineages respectively. Overall, findings from this study will contribute to ongoing efforts in adipose tissue engineering as well as provide new insights into the role of the ECM and cues provided by the immediate microenvironment for stem cell differentiation. - Highlights: • Native adipose tissue ECM coated on 2D TCP triggers adipogenesis in both ASCs and Bm-MSCs. • A 3D microenvironment with similar stiffness to adipose tissue induces adipogenic differentiation of ASCs. • ASCs cultured in 3D alginate scaffolds exhibit predisposition to adipogenesis. • Bm-MSCs cultured in 3D alginate scaffolds exhibit predisposition to osteogenesis. • The native microenvironment of the cells affects their differentiation behaviour in vitro.

Comparative study of adipose-derived stem cells and bone marrow-derived stem cells in similar microenvironmental conditions

International Nuclear Information System (INIS)

Guneta, Vipra; Tan, Nguan Soon; Chan, Soon Kiat Jeremy; Tanavde, Vivek; Lim, Thiam Chye; Wong, Thien Chong Marcus; Choong, Cleo

2016-01-01

Mesenchymal stem cells (MSCs), which were first isolated from the bone marrow, are now being extracted from various other tissues in the body, including the adipose tissue. The current study presents systematic evidence of how the adipose tissue-derived stem cells (ASCs) and bone marrow-derived mesenchymal stem cells (Bm-MSCs) behave when cultured in specific pro-adipogenic microenvironments. The cells were first characterized and identified as MSCs in terms of their morphology, phenotypic expression, self-renewal capabilities and multi-lineage potential. Subsequently, the proliferation and gene expression profiles of the cell populations cultured on two-dimensional (2D) adipose tissue extracellular matrix (ECM)-coated tissue culture plastic (TCP) and in three-dimensional (3D) AlgiMatrix® microenvironments were analyzed. Overall, it was found that adipogenesis was triggered in both cell populations due to the presence of adipose tissue ECM. However, in 3D microenvironments, ASCs and Bm-MSCs were predisposed to the adipogenic and osteogenic lineages respectively. Overall, findings from this study will contribute to ongoing efforts in adipose tissue engineering as well as provide new insights into the role of the ECM and cues provided by the immediate microenvironment for stem cell differentiation. - Highlights: • Native adipose tissue ECM coated on 2D TCP triggers adipogenesis in both ASCs and Bm-MSCs. • A 3D microenvironment with similar stiffness to adipose tissue induces adipogenic differentiation of ASCs. • ASCs cultured in 3D alginate scaffolds exhibit predisposition to adipogenesis. • Bm-MSCs cultured in 3D alginate scaffolds exhibit predisposition to osteogenesis. • The native microenvironment of the cells affects their differentiation behaviour in vitro.

Evaluation of outgassing, tear strength, and detail reproduction in alginate substitute materials.

Science.gov (United States)

Baxter, R T; Lawson, N C; Cakir, D; Beck, P; Ramp, L C; Burgess, J O

2012-01-01

To compare three alginate substitute materials to an alginate impression material for cast surface porosity (outgassing), tear strength, and detail reproduction. Detail reproduction tests were performed following American National Standards Institute/American Dental Association (ANSI/ADA) Specification No. 19. To measure tear strength, 12 samples of each material were made using a split mold, placed in a water bath until testing, and loaded in tension until failure at a rate of 500 mm/min using a universal testing machine. For cast surface porosity testing, five impressions of a Teflon mold with each material were placed in a water bath (37.8°C) for the in-mouth setting time and poured with vacuum-mixed Silky Rock die stone at 5, 10, 30, and 60 minutes from the start of mixing. The gypsum samples were analyzed with a digital microscope for surface porosity indicative of hydrogen gas release by comparing the surface obtained at each interval with four casts representing no, little, some, and significant porosity. Data analysis was performed using parametric and Kruskal-Wallis analysis of variance (ANOVA), Tukey/Kramer post-hoc tests (α=0.05), and individual Mann-Whitney U tests (α=0.0167). All alginate substitute materials passed the detail reproduction test. Tear strength of the alginate substitute materials was significantly better than alginate and formed three statistically different groups: AlgiNot had the lowest tear strength, Algin-X Ultra had the highest tear strength, and Position Penta Quick had intermediate tear strength. Significant variation in outgassing existed between materials and pouring times (palginate substitute materials exhibited the least outgassing and cast porosity 60 minutes after mixing. Detail reproduction and tear strength of alginate substitute materials were superior to traditional alginate. The outgassing effect was minimal for most materials tested. Alginate substitute materials are superior replacements for irreversible

Basic investigation of the laminated alginate impression technique: Setting time, permanent deformation, elastic deformation, consistency, and tensile bond strength tests.

Science.gov (United States)

Kitamura, Aya; Kawai, Yasuhiko

2015-01-01

Laminated alginate impression for edentulous is simple and time efficient compared to border molding technique. The purpose of this study was to examine clinical applicability of the laminated alginate impression, by measuring the effects of different Water/Powder (W/P) and mixing methods, and different bonding methods in the secondary impression of alginate impression. Three W/P: manufacturer-designated mixing water amount (standard), 1.5-fold (1.5×) and 1.75-fold (1.75×) water amount were mixed by manual and automatic mixing methods. Initial and complete setting time, permanent and elastic deformation, and consistency of the secondary impression were investigated (n=10). Additionally, tensile bond strength between the primary and secondary impression were measured in the following surface treatment; air blow only (A), surface baking (B), and alginate impression material bonding agent (ALGI-BOND: AB) (n=12). Initial setting times significantly shortened with automatic mixing for all W/P (p<0.05). The permanent deformation decreased and elastic deformation increased as high W/P, regardless of the mixing method. Elastic deformation significantly reduced in 1.5× and 1.75× with automatic mixing (p<0.05). All of these properties resulted within JIS standards. For all W/P, AB showed a significantly high bonding strength as compared to A and B (p<0.01). The increase of mixing water, 1.5× and 1.75×, resulted within JIS standards in setting time, suggesting its applicability in clinical setting. The use of automatic mixing device decreased elastic strain and shortening of the curing time. For the secondary impression application of adhesives on the primary impression gives secure adhesion. Copyright © 2014 Japan Prosthodontic Society. Published by Elsevier Ltd. All rights reserved.

LANGUAGE OF LOVE IN MARCELIJUS MARTINAITIS "ATMINTYS"

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Kristina Bačiulienė

2014-10-01

Full Text Available The notion of transcendental love in the context of “the eternal return”, the motif of Eros, and the image of a woman are discussed in Marcelijus Martinaitis’s book "Atmintys: meilės lyrikos albumas" (eng. "Reminiscences: the album of love lyrics" (2008. The methods of mythopoetic thought, comparativistics, and interpretative mind have been applied. On the basis of the Bible, mythology, the notion of and relation between Eros and Love, also combining notions developed by philosophers as Nikolai Berdyaev, Erich Fromm, Vladimir Solovyev, phenomenologists as Algis Mickūnas, Mircea Eliade, Plato, and insights by popes Paul VI and Benedict XVI, a concept which might be useful in deciphering the language of love in Marcelijus Martinaitis’s book is introduced. The primary code of the album of love is the concept of memory and the notion of love as seen by Oskaras Milašius, while the secondary code relates to Dante, chivalric romance of Provence troubadours, legends of King Arthur, Celtic mythology. The genre of M. Martinaitis’s reminiscences was born when the poet was studying in Gervinės, and the first collection of Reminiscences appeared in 1986. The text of Reminiscences was seen as hermetic, intertextual, reflecting the act of cosmogony and antropogony in permanent space-time. The process of word re-creation is associated with archetypal consciousness, and the cycle of “the eternal return” prevails. Love is perceived as transcendental and existential basis, a purpose to merge with God, God’s gift to humanity, and the vision of love object. Eros (passion is ambivalent: it is earthly / heavenly, sinful / divine, creating / destructive, and driving power of creation. A woman is seen as earthly / sensual, divine / unreachable. An issue which motif (Christian or courtesan literature dominates in the album of love lyrics is discussed. Album is of great interest as an immanent text.

Isolation and characterization of Wharton’s jelly-derived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined serum-free three-dimensional system

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Cardoso Tereza C

2012-05-01

bovine-derived UC-WJ cells was included in the culture which demonstrated the immunossupression profile typically observed among isolated mesenchymal cells from other species. After classified the UC-WJ cells as mesenchymal stromal phenotype the in vitro 3D cultures was performed using the AlgiMatrix® protocol. Based on the size of spheroids (283,07 μm ± 43,10 μm we found that three weeks of culture was the best period to growth the UC-WJ cells on 3D dimension. The initial cell density was measured and the best value was 1.5 × 106 cells/well. Conclusions We described for the first time the isolation and characterization of UC-WJ cells in a serum-free condition and maintenance of primitive mesenchymal phenotype. The culture was stable under 60 consecutive passages with no genetic abnormalities and proliferating ratios. Taken together all results, it was possible to demonstrate an easy way to isolate and culture of bovine-derived UC-WJ cells under 2D and 3D serum-free condition, from fetal adnexa with a great potential in cell therapy and biotechnology.

Editorial. Kultūros reiškiniai šiuolaikinių informacijos ir komunikacijos technologijų sąlygomis: nuo meno ir televizijos iki politikos

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Jovilė Barevičiūtė

2015-10-01

Full Text Available Šiame žurnalo numeryje aptariami kai kurie daugiaaspekčiai šiuolaikinės kultūros reiškiniai, apimant tokius jų, kaip menas, televizija, politika ir kt. Medijos ir komunikacija nūdien intensyviai skverbiasi kone į visas darbo ir laisvalaikio veiklos, viešojo ir privataus sektorių sritis, reprezentuodamos dinamišką ir netolygią gyvenamojo pasaulio tikrovę. Medijuotoji komunikacija vis labiau tampa dominuojančia socializacijos forma, ji išstumia gyvąją komunikaciją: kuo toliau, tuo labiau šis reiškinys nusako ne tik jaunimo, bet ir brandaus amžiaus žmonių šiandienį bendravimo būdą. Tad akivaizdu, kad XXI a. žmogus vis labiau pasineria į įvairių medijų tinklus, apraizgančius jo gyvenimą ir tampančius pagrindine socialios ir visavertės eg zistencijos sąlyga. Tokios socialinės institucijos, kaip menas, televizija, politika, religija, šeima ir daugelis kitų, nūdien jau nebėra tokios savarankiškos, kaip, tarkime, prieš dešimt, dvidešimt ar daugiau metų. Jų suverenitetą daugiausia transformuoja būtent medijos, šioms institucijoms diktuojančios savas sąlygas ir primetančios savas taisykles. Kitaip tariant, medijos tampa tokios galingos, kad ima steigti savuosius dėsnius, kuriems vis labiau paklūsta tradicinė gyvosios socializacijos aplinka. Taip kyla daugybė diskutuotinų ir ginčytinų klausimų, paliečiančių socialinių institucijų apibrėžties, autonomijos ir suvereniteto aspektus. Šiuos aspektus iš įvairių perspektyvų gvildena ir šio numerio autoriai. Eugenija Krukauskienė ir Viktorija Žilinskaitė-Vytienė savo straipsnyje aptaria kultūros vartojimo klausimus tirdamos, kaip šiuolaikinio lietuviškojo kino meno pavyzdžius suvokia ir vertina tam tikrų amžiaus kategorijų jaunimas. Algis Mickūnas gilinasi į filosofinius šiuolaikinių diskursų klausimus, pasirinkdamas kūniškumo, lytėjimo ir taktilikos aspektus, glaudžiai susijusius su medijuotosios komunikacijos tema

INVESTIGATION OF PRIMARY FIFTH GRADE STUDENTS’KNOWLEDGE AND PERCEPTION LEVEL OF NASREDDIN HODJA AND KELOĞLAN İLKÖĞRETİM BEŞİNCİ SINIF ÖĞRENCİLERİNİN NASREDDİN HOCA VE KELOĞLAN’A İLİŞKİN BİLGİ VE ALGI DÜZEYLERİNİN İNCELENMESİ

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Erol DURAN

2012-06-01

Full Text Available This study aims to investigate how primary fifth grade students'levels of knowledge and perception about Nasreddin Hodja and Keloğlan. This study were tried to find answer of these question: “Which is the level of students' knowledge about Nasreddin Hodja and Keloğlan?”, “Which sources students learned Keloğlan tales?”, “Can Students detect correctly perceive the message to be given in jokes or tales”. In this study, the descriptive model; content and descriptive analysis technique was used. The study group is 172 primary fifth grade students chosen at random in Uşak city center. In this study, a data collection tool, Investigation of Primary Fifth Grade Students’ Knowledge and Perception Related toNasreddin Hodja and Keloğlan, developed by the researcher, was used. According to the survey, students define Nasreddin Hodja as “funny, reverse, donkey riding, witty and knowledgeable”. But some students define Nasreddin Hodja as "a liar and deceitful. This rate for the number of students cannot be underestimated. Students define Keloğlan as “bald, cartoon character and intelligent”. In addition, in the students responses, Keloğlan "lazy and disobedient" features stand out. More than half of the students have been failed or very failed to percept the message to be given in Nasreddin Hodja jokes. However, more than half of the students have been success or very success to percept the message to be given in Keloğlan tales. İlköğretim beşinci sınıf öğrencilerinin Nasreddin Hoca ve Keloğlan ile ilgili bilgi ve algı düzeylerini belirlemeyi amaçlayan bu araştırmada, “Öğrencilerin Nasreddin Hoca ve Keloğlan ile ilgili bilgi masallarını hangi kaynaklardan öğrendikleri” ve “Fıkralarda veya masallarda verilmek istenen mesajı doğru algılayıp algılayamadıkları” araştırılmaya çalışılmıştır. Araştırmada, betimsel tarama modeli; içerik ve betimsel analiz tekniği kullanılmıştır. Araştırmanın çalışma grubunu, Uşak il merkezi ilköğretim okullarına devam eden beşinci sınıf öğrencileri içerisinden rastgele yolla seçilen 172 öğrenci oluşturmaktadır. Araştırmada veri toplama aracı olarak, araştırmacı tarafından geliştirilen “İlköğretim Beşinci Sınıf Öğrencilerinin Nasreddin Hoca ve Keloğlan ile İlgili Bilgi ve Algı Düzeylerini Belirleme Envanteri” kullanılmıştır. Araştırma sonuçlarına göre öğrenciler, Nasreddin Hoca’yı, “komik, eşeğe ters binen hazırcevap ve bilgili” şeklinde tanımlamaktadırlar. Ancak Nasreddin Hoca’yı, “yalancı ve hilekâr” olarak tanımlayan öğrenci sayısı da azımsanmayacak orandadır. Öğrenciler Keloğlanı daha çok “kel kafalı, çizgi film kahramanı ve akıllı”, şeklinde tanımlamaktadırlar. Ayrıca, Keloğlanın “tembel ve söz dinlemeyen” özellikleri de, öğrencilerin cevaplarında öne çıkmaktadır. Öğrencilerin yarısından fazlasının Nasreddin Hoca fıkralarında verilmek istenen mesajı algılamada “çok başarısız veya başarısız” oldukları; Keloğlan masallarında verilmek istenen mesajı algılamada ise öğrencilerin yarısından fazlasının “çok başarılı veya başarılı” oldukları tespit edilmiştir.

Editorial. Dialogue, Communication and Collaboration: Aspects of Philosophy and Communication

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Jovilė Barevičiūtė

2016-03-01

versus Antirealism in Science Education is a contribution by Seungbae Park, in which he attempts to define how the dialogue between teachers and students is possible, as he takes the position stating that the doctrine of scientific realism is much more effective than provided opportunities of scientific antirealism. And finally, Algis Mickūnas, in his article The Different Other and Dialogue, discusses the reasons why members of different communities find it difficult to establish dialogue-based relationships and why in some cases they remain imprisoned in the state of a monologue. This issue of the journal presents a truly wide field of investigations into opportunities and obstacles for communication, interaction and collaboration. It is pleasing to see that representatives of various humanities and social sciences joined the same dialogue. Looking forward to the productive insights in the future, the Editor would like to express her gratitude to the authors of this issue.

Exploring Unconventional Hydrocarbons in the Makó Trough, Pannonian basin, Hungary: Results and Challenges

Science.gov (United States)

Horvath, Anita; Bada, Gabor; Szafian, Peter; Sztano, Orsolya; Law, Ben; Wallis, Rod

2010-05-01

subsidence (Endrőd Formation). The basin was then almost completely filled by the turbidites of a prograding delta system (Szolnok Formation), followed by the sediments from the pro-delta (Algyő Formation) and the delta plain (Újfalu Formation). During the Plio- and Pleistocene, the area continued to subside at a decreased rate, providing a limited accumulation space filled by a thick sequence of alluvial deposits similar to the paleo-environment of the Tisza River and its tributaries prior to regulation. Our modeling of basin evolution in terms of subsidence, thermal and maturation history based on the results of geochemical, petrophysical, and paleontological investigations reveals that the organic matter accumulated in the trough underwent exceptionally rapid maturation during the last 5 to 7 million years. In fact, source rocks continue to mature at present time, and the hydrocarbons they generate continue to migrate and trap. The rather young age of the Pannonian Basin and its hydrocarbon system makes it quite different from the other examples of basin-centered gas accumulation, such as the classic Rocky Mountains in the U.S., or the Karoo Basin in South Africa, the Beetaloo Basin in Australia, making up the exploration portfolio of Falcon.