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Sample records for aukalnis algis kadzys

  1. Development of a commercial Automated Laser Gas Interface (ALGI) for AMS

    Energy Technology Data Exchange (ETDEWEB)

    Daniel, R. [National Electrostatics Corporation, Middleton, WI 53562 (United States); Mores, M., E-mail: nec@pelletron.com [National Electrostatics Corporation, Middleton, WI 53562 (United States); Kitchen, R.; Sundquist, M.; Hauser, T.; Stodola, M. [National Electrostatics Corporation, Middleton, WI 53562 (United States); Tannenbaum, S.; Skipper, P.; Liberman, R. [Dept. of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 (United States); Young, G.; Corless, S. [Drug Metabolism and Pharmacokinetics, GlaxoSmithKline Research and Development Ltd., Ware (United Kingdom); Tucker, M. [MGT Systems, Chapel Lane, Eastbridge, Suffolk (United Kingdom)

    2013-01-15

    National Electrostatics Corporation (NEC), Massachusetts Institute of Technology (MIT), and GlaxoSmithKline (GSK) collectively have been developing an interface to introduce CO{sub 2} produced by the laser combustion of liquid chromatograph eluate deposited on a CuO substrate directly into the ion source of an AMS system, thereby bypassing the customary graphitization process. The Automated Laser Gas Interface (ALGI) converts dried liquid samples to CO{sub 2} gas quickly and efficiently, allowing 96 samples to be measured in as little as 16 h. {sup 14}C:{sup 12}C ratios stabilize typically within 2 min of analysis time per sample. Presented is the recent progress of NEC's ALGI, a stand-alone accessory to an NEC gas-enabled multi-cathode source of negative ions by Cs sputtering (MC-SNICS) ion source.

  2. Juhtimisülesanne : ja kalad saavad ka kinga / Ergo Metsla ; kommenteerinud Algis Perens, Liis Luuk

    Index Scriptorium Estoniae

    Metsla, Ergo, 1968-

    2010-01-01

    Juhtimisülesanne, kus ettevõte on pärast turu kokkulanemist sattunud majanduslikesse raskustesse ning ettevõtte juhid on valiku ees, kas lõhkuda aastaid koos töötanud meeskond ja koondada töötajaid või leida firma püsimajäämiseks mõni muu variant. Lahendust otsivad konsultantsioonifirma Helvetia Balti Partnerid juhatuse esimees Algis Perens ja Kalev SPA Veekeskuse müügi- ja turundusassistent Liis Luuk

  3. The Reflection of Quantum Aesthetics in Algis Mickūnas Cosmic Philosophy

    Directory of Open Access Journals (Sweden)

    Auridas Gajauskas

    2011-04-01

    Full Text Available Quantum Aesthetics phenomenon was formed in Spain, at the end of the twentieth centure. The paper analyzes this movement in the context of Algis Mickūnas phenomenological cosmic philosophy. Movement initiator is a Spanish novelist Gregorio Morales. The study is divided into two parts: the first part presents aesthetic principles of the quantum, relationship between new aesthetics and theories of quantum mechanics, physics and other sciences. The paper also examines the similarities of quantum aesthetics and New Age movements. The second part presents cosmic - phenomenological reflection of quantum theory of beauty. Mickūnas philosophical position combines theory of "eternal recurrence", "the bodily nature of consciousness", "the cosmic dance", theory of "dynamic fields" and quantum approach to aesthetics and the Universe. Summa Summarum he writes that "the conception of quantum aesthetics is involved in the composition of the rhythmic, cyclical and mood dimensioned and tensed world". 

  4. Etat de Stress Post-traumatique lié à l'accouchement et Algies pelviennes chroniques

    OpenAIRE

    GEFFROY- GRECO , Gisele

    2005-01-01

    Objectif : Rechercher un lien entre un accouchement objectivement stressant (césarienne ou forceps pour anomalies du rythme cardiaque fœtal, hémorragie de la délivrance) et des algies pelviennes chroniques (dysménorrhées, douleurs pelviennes non cycliques, dyspareunies) dans le post-partum tardif (au-delà d'un an).Méthode : auto-questionnnaire ; enquête exposés/non-exposés ; comparaison entre accouchement stressant et accouchement normal. Résultats : taux de réponse 58,3%. 253 patientes dans ...

  5. AlgiMatrix™ based 3D cell culture system as an in-vitro tumor model for anticancer studies.

    Directory of Open Access Journals (Sweden)

    Chandraiah Godugu

    Full Text Available Three-dimensional (3D in-vitro cultures are recognized for recapitulating the physiological microenvironment and exhibiting high concordance with in-vivo conditions. Taking the advantages of 3D culture, we have developed the in-vitro tumor model for anticancer drug screening.Cancer cells grown in 6 and 96 well AlgiMatrix™ scaffolds resulted in the formation of multicellular spheroids in the size range of 100-300 µm. Spheroids were grown in two weeks in cultures without compromising the growth characteristics. Different marketed anticancer drugs were screened by incubating them for 24 h at 7, 9 and 11 days in 3D cultures and cytotoxicity was measured by AlamarBlue® assay. Effectiveness of anticancer drug treatments were measured based on spheroid number and size distribution. Evaluation of apoptotic and anti-apoptotic markers was done by immunohistochemistry and RT-PCR. The 3D results were compared with the conventional 2D monolayer cultures. Cellular uptake studies for drug (Doxorubicin and nanoparticle (NLC were done using spheroids.IC(50 values for anticancer drugs were significantly higher in AlgiMatrix™ systems compared to 2D culture models. The cleaved caspase-3 expression was significantly decreased (2.09 and 2.47 folds respectively for 5-Fluorouracil and Camptothecin in H460 spheroid cultures compared to 2D culture system. The cytotoxicity, spheroid size distribution, immunohistochemistry, RT-PCR and nanoparticle penetration data suggested that in vitro tumor models show higher resistance to anticancer drugs and supporting the fact that 3D culture is a better model for the cytotoxic evaluation of anticancer drugs in vitro.The results from our studies are useful to develop a high throughput in vitro tumor model to study the effect of various anticancer agents and various molecular pathways affected by the anticancer drugs and formulations.

  6. AlgiMatrix™-Based 3D Cell Culture System as an In Vitro Tumor Model: An Important Tool in Cancer Research.

    Science.gov (United States)

    Godugu, Chandraiah; Singh, Mandip

    2016-01-01

    Routinely used two-dimensional cell culture-based models often fail while translating the observations into in vivo models. This setback is more common in cancer research, due to several reasons. The extracellular matrix and cell-to-cell interactions are not present in two-dimensional (2D) cell culture models. Diffusion of drug molecules into cancer cells is hindered by barriers of extracellular components in in vivo conditions, these barriers are absent in 2D cell culture models. To better mimic or simulate the in vivo conditions present in tumors, the current study used the alginate based three-dimensional cell culture (AlgiMatrix™) model, which resembles close to the in vivo tumor models. The current study explains the detailed protocols involved in AlgiMatrix™ based in vitro non-small-cell lung cancer (NSCLC) models. The suitability of this model was studied by evaluating, cytotoxicity, apoptosis, and penetration of nanoparticles into the in vitro tumor spheroids. This study also demonstrated the effect of EphA2 receptor targeted docetaxel-loaded nanoparticles on MDA-MB-468 TNBC cell lines. The methods section is subdivided into three subsections such as (1) preparation of AlgiMatrix™-based 3D in vitro tumor models and cytotoxicity assays, (2) free drug and nanoparticle uptake into spheroid studies, and (3) western blot, IHC, and RT-PCR studies.

  7. Geology of Kubi Algi and Derati mountains, pantellerite bodies of Miocene age from the northern part of the Kenyan Rift Valley

    Science.gov (United States)

    Watkins, R. T.

    The small isolated peaks of Kubi Algi and Derati on the periphery of the Koobi Fora basin, to the north-east of Lake Turkana in northern Kenya, are remnants of silicic peralkaline volcanic centres. Detailed geological maps of the areas of the two mountains are presented. Both are massive bodies of generally aphyric, microgranular pantellerite sharing similar petrography and chemistry. Kubi Algi shows evidence of having formed as an extrusive dome and is considered the source of local pantellerite lava flows, here designated the Il Burrka Formation. Derati mountain can best be interpreted as a denuded plug of a second extrusive centre. The volcanoes were active in the middle Miocene towards the end of a period of regional magmatism extending from late-Oligocene times. The pantellerites are holocrystalline and thus contrast with the normally glassy over-saturated peralkaline rocks from the East African rifts, including older pyroclastic pantellerites of the northern Lake Turkana region. Despite being very finely crystalline, they show mineralogical features seen elsewhere in more slowly cooled, deep-seated, peralkaline granites. A very broad range of feldspar compositions present in the rocks is explained by the interaction of groundwater with the rapidly cooling magma. Of additional interest is the abundance of aegirine, present as a product of primary magmatic crystallization and, in the Derati rock, as a hydrothermal mineral. It contains significant but highly variable amounts of titanium and zirconium, the latter broadly equivalent to typical maximum concentrations reported from peralkaline intrusive complexes.

  8. YAYLI ÇALGI EĞİTİMİNDE MÜZİKAL İFADENİN GELİŞTİRİLMESİ

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    Hasan Hakan Okay

    2013-04-01

    Full Text Available In the instrument training process, the development of musical expression is an important requirement. This research aims to determine the effect of the method that is easy to understand that will allow students to create their own strategies for the development of musical expression and which takes its source from the relationship between music and language also the relationship between the instrumental music and song. Studies have indicated with neurophysiological evidence that the language and the music carries a very important partnerships. Instrumental music, stands out as a case that takes its source from song when assessed with the understanding of instruments oppointed as a soloist. Therefore, the method is designed based on this understanding to recognize music and instrumental music. Theoretical aspect of the method used is given in the research report in detail due to its original structure.Pretest - posttest experimental and control group experimental research design was used. The research were applied in Balıkesir University Necatibey Education Faculty Education Department of Fine Arts Music Education Branch on Fall Semester of the 2009-2010 Academic Year in 10-week training period with participation of 20 students. Datas were gathered by “Musical Expression Performance Scale”. In the end of experimental study has resulted in favor of the experimental group.

  9. INVESTIGATION OF PRIMARY FIFTH GRADE STUDENTS’KNOWLEDGE AND PERCEPTION LEVEL OF NASREDDIN HODJA AND KELOĞLAN İLKÖĞRETİM BEŞİNCİ SINIF ÖĞRENCİLERİNİN NASREDDİN HOCA VE KELOĞLAN’A İLİŞKİN BİLGİ VE ALGI DÜZEYLERİNİN İNCELENMESİ

    Directory of Open Access Journals (Sweden)

    Erol DURAN

    2012-06-01

    Full Text Available This study aims to investigate how primary fifth grade students'levels of knowledge and perception about Nasreddin Hodja and Keloğlan. This study were tried to find answer of these question: “Which is the level of students' knowledge about Nasreddin Hodja and Keloğlan?”, “Which sources students learned Keloğlan tales?”, “Can Students detect correctly perceive the message to be given in jokes or tales”. In this study, the descriptive model; content and descriptive analysis technique was used. The study group is 172 primary fifth grade students chosen at random in Uşak city center. In this study, a data collection tool, Investigation of Primary Fifth Grade Students’ Knowledge and Perception Related toNasreddin Hodja and Keloğlan, developed by the researcher, was used. According to the survey, students define Nasreddin Hodja as “funny, reverse, donkey riding, witty and knowledgeable”. But some students define Nasreddin Hodja as "a liar and deceitful. This rate for the number of students cannot be underestimated. Students define Keloğlan as “bald, cartoon character and intelligent”. In addition, in the students responses, Keloğlan "lazy and disobedient" features stand out. More than half of the students have been failed or very failed to percept the message to be given in Nasreddin Hodja jokes. However, more than half of the students have been success or very success to percept the message to be given in Keloğlan tales. İlköğretim beşinci sınıf öğrencilerinin Nasreddin Hoca ve Keloğlan ile ilgili bilgi ve algı düzeylerini belirlemeyi amaçlayan bu araştırmada, “Öğrencilerin Nasreddin Hoca ve Keloğlan ile ilgili bilgi masallarını hangi kaynaklardan öğrendikleri” ve “Fıkralarda veya masallarda verilmek istenen mesajı doğru algılayıp algılayamadıkları” araştırılmaya çalışılmıştır. Araştırmada, betimsel tarama modeli; içerik ve betimsel analiz tekniği kullanılmıştır. Araştırmanın çalışma grubunu, Uşak il merkezi ilköğretim okullarına devam eden beşinci sınıf öğrencileri içerisinden rastgele yolla seçilen 172 öğrenci oluşturmaktadır. Araştırmada veri toplama aracı olarak, araştırmacı tarafından geliştirilen “İlköğretim Beşinci Sınıf Öğrencilerinin Nasreddin Hoca ve Keloğlan ile İlgili Bilgi ve Algı Düzeylerini Belirleme Envanteri” kullanılmıştır. Araştırma sonuçlarına göre öğrenciler, Nasreddin Hoca’yı, “komik, eşeğe ters binen hazırcevap ve bilgili” şeklinde tanımlamaktadırlar. Ancak Nasreddin Hoca’yı, “yalancı ve hilekâr” olarak tanımlayan öğrenci sayısı da azımsanmayacak orandadır. Öğrenciler Keloğlanı daha çok “kel kafalı, çizgi film kahramanı ve akıllı”, şeklinde tanımlamaktadırlar. Ayrıca, Keloğlanın “tembel ve söz dinlemeyen” özellikleri de, öğrencilerin cevaplarında öne çıkmaktadır. Öğrencilerin yarısından fazlasının Nasreddin Hoca fıkralarında verilmek istenen mesajı algılamada “çok başarısız veya başarısız” oldukları; Keloğlan masallarında verilmek istenen mesajı algılamada ise öğrencilerin yarısından fazlasının “çok başarılı veya başarılı” oldukları tespit edilmiştir.

  10. Former health care vice minister sentenced / Rokas M. Tracevskis

    Index Scriptorium Estoniae

    Tracevskis, Rokas M.

    2010-01-01

    Leedu endise tervishoiu aseministri Arturas Skikase kohtuotsusest seoses altkäemaksu võtmisega. Arturas Skikas tunnistas üles, et soovis saada verekeskuse direktori käest 5800 eurot. Tervishoiuminister Algis Caplikas jääb ametisse

  11. Analytic Hierarchy Process for Personalising Environmental Information

    Science.gov (United States)

    Kabassi, Katerina

    2014-01-01

    This paper presents how a Geographical Information System (GIS) can be incorporated in an intelligent learning software system for environmental matters. The system is called ALGIS and incorporates the GIS in order to present effectively information about the physical and anthropogenic environment of Greece in a more interactive way. The system…

  12. Suurim kliendiinfo leke kindlustuses / Sten-Aleks Pihlak

    Index Scriptorium Estoniae

    Pihlak, Sten-Aleks

    2008-01-01

    Eesti suurimat kindlustusmaaklerit Iizi kahtlustatakse koos IT-arendusfirmaga Indium Eesti kindlustusturu suurimas infolekkes. Vt. samas: Intervjuu OÜ Indium juhi Indrek Soomestega. IT-mees: minul oli väike osa; kommenteerivad Sergei Vahnitski, Risto Rossar, Enn Saar; Algis Suik. Lisa: Infolekke skeem; Kuidas sündmused arenesid. Vt. lisaks: IIZI kindlustusfirmal lasub kahtlus. Algatati kriminaalmenetlus. Äripäev 16. juuli lk. 11

  13. Civitas: Toward a Secure Voting System

    Science.gov (United States)

    2008-05-01

    voting, we believe that remote vot- ing is the right problem to solve. One of our goals was therefore to strike a reasonable compromise between enabling...versions of this work. References [1] Ben Adida . Advances in Cryptographic Voting Systems. PhD thesis, MIT, Aug. 2006. [2] Roberto Araújo, Sébastien...3] Association for Computing Machinery. SIG elections. http://www.acm.org/sigs/elections, 2007. [4] Jonathan Bannet, David W. Price , Algis Rudys

  14. Pixe analysis of Cu,Zn,Hg and Cd in mussels samples in bay of Algiers

    International Nuclear Information System (INIS)

    Benamar, M.A.; Tchantchane, A.; Benouali, N.; Azbouche, A.; Tobbeche, S.

    1995-01-01

    The purpose of our work is the elaboration of and absolute technique for determination of trace elements in biological matrices by means of Pixe analysis. We are interested in the determination of heavy metals in mussels samples taken from differents sits of algies coast (Cu,Zn,Cd and Hg). The reason of our choise is the element toxicity and the possible contamination of the marine environment

  15. Evaluation of alginate microspheres for mesenchymal stem cell engraftment on solid organ

    OpenAIRE

    Trouche, E.; Girod Fullana, S.; Mias, C.; Ceccaldi, C.; Tortosa, F.; Seguelas, M. H.; Calise, D.; Parini, A.; Cussac, D.; Sallerin, B.

    2010-01-01

    Mesenchymal stem cells (MSCs) may be used as a cell source for cell therapy of solid organs due to their differentiation potential and paracrine effect. Nevertheless, optimization of MSC-based therapy needs to develop alternative strategies to improve cell administration and efficiency. One option is the use of alginate microencapsulation, which presents an excellent biocompatibility and an in vivo stability. As MSCs are hypoimmunogenic, it was conceivable to produce microparticles with [algi...

  16. Application of Electrostatic Extrusion – Flavour Encapsulation and Controlled Release

    OpenAIRE

    Manojlovic, Verica; Rajic, Nevenka; Djonlagic, Jasna; Obradovic, Bojana; Nedovic, Viktor; Bugarski, Branko

    2008-01-01

    The subject of this study was the development of flavour alginate formulations aimed for thermally processed foods. Ethyl vanilline was used as the model flavour compound. Electrostatic extrusion was applied for the encapsulation of ethyl vanilline in alginate gel microbeads. The obtained microbeads with approx. 10 % w/w of ethyl vanilline encapsulated in about 2 % w/w alginate were uniformly sized spheres of about 450 ?m. Chemical characterization by H-NMR spectroscopy revealed that the algi...

  17. Armenian Demons Called Kaj: Image and Name

    Directory of Open Access Journals (Sweden)

    Armen Y. Petrosyan

    2018-03-01

    Full Text Available The article provides a study of Armenian demons, the kajs, and their superior deity — the dragonslayer Vahagn, in the historical-etymological and ethno-cultural perspective. Specific features of kajs and their leader as imagined by ancient people are identifi ed: they resemble vishap-dragons and devs, live in the mountains and have temples there, make war, hunt, steal wheat and wine from people, love music, arrange weddings, drive people crazy, braid horsehair, and so on. Clear linkages are drawn between Armenian kajs and their leader with the mythological characters of Indo-European (Iranian, Indian traditions. In Armenian, the fi rst meaning of k‘aǰ is ‘good, select; of fi ne / better quality’. This is exactly how some Armenian spirits, including kajs, are often referred to — as “good” — mezane laver, mezne ałekner ‘better than us,’ ałek manuk ‘good youngster / warrior’. This is also true to some other traditions: the Persian az mā behtarān, German Gude (cf. also the names of Indian vasu and sādhya. The article develops the etymology of the name k‘aǰ (a corrected version of that proposed by H. Pedersen in 1906: *swo-sHdhyo- — ‘self / own + right / righteous,’ i.e. ‘good’. This image passed into the Georgian and Ossetian traditions (Georgian kajs, Ossetian kadzi.

  18. Cloning and Expression of Three New Azotobacter vinelandii Genes Closely Related to a Previously Described Gene Family Encoding Mannuronan C-5-Epimerases

    OpenAIRE

    Svanem, Britt Iren Glærum; Skjåk-Bræk, Gudmund; Ertesvåg, Helga; Valla, Svein

    1999-01-01

    The cloning and expression of a family of five modular-type mannuronan C-5-epimerase genes from Azotobacter vinelandii (algE1 to -5) has previously been reported. The corresponding proteins catalyze the Ca2+-dependent polymer-level epimerization of β-d-mannuronic acid to α-l-guluronic acid (G) in the commercially important polysaccharide alginate. Here we report the identification of three additional structurally similar genes, designated algE6, algE7, and algY. All three genes were sequenced...

  19. Difficultés de la surveillance épidémiologique de la rougeole en Afrique : exemple de la Côte d'Ivoire

    OpenAIRE

    Rey, Jean-Loup; Trolet, C.; Soro, B.; Cunin, P.; Merouze, F.

    1991-01-01

    En zone tropicale, les cas de rougeole sont souvent sous-déclarés mais les auteurs exposent deux épidémies qui ont été considérées au début comme des manifestations de rougeole. La première épidémie s'est manifestée comme une épidémie de viroses à Chikungunya avec éruptions, hyperthermie et algies importantes, elle était due à un arbovirus Igbo-Ora. Dans l'autre cas il s'agissait d'enfants atteints d'éruptions avec hyperthermie et adénopathies évoquant la rubéole. Les auteurs évoquent la poss...

  20. HEALTH WORKER AND FIBROMYALGIA: relationship between pain, and physical activity

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    Andréa Cristina Costa

    2012-11-01

    Full Text Available The objective of the present article is to address the concept of fibromyalgie, as well as the painful symptomatology and physical exercise as a therapeutic manner, stressing the importance of this alteration in the field of workers health. It is in fact a revision, carried out through consultations of scientific articles selected in periodicals indexed at the basis of the Scientific Electronic Library Online – SciELO and PubMed. In spite of the absence of scientific evidences that point to physical efforts as factors that cause fibromyalgie, there are some studies relating the repetitive microtrauma stemming from work as ethiological agent. It is characterized as a syndrome whose main symptom is pain, which is utilized as a manner of evaluation and pathology follow-up, through such tools as scales. With regard to the treatment, physical exercises are able to minimize the algie and other symptoms. Finally, all related research works pursue correct and deep understanding of this pathology, so as to define more appropriate treatments, whilst keeping the person’s position as worker, under the assistance of specialized workers health services, thus providing quality of life.

  1. Paleochemistry of Plio-Pleistocene Lake Turkana, Kenya. [Alkalinity

    Energy Technology Data Exchange (ETDEWEB)

    Cerling, T.

    1979-01-01

    The paleochemisry of Plio-Pleistocene Lake Turkana can be estimated by using the chemistry of lakes from the Eastern Rift of Africa as an analogue. Most modern East Africa lakes occupy closed basins; their chemistries follow an evaporation trend defined by the precipitation of certain mineral phases with increasing alkalinity. Estimates of paleoalkalinity can be used to closely estimate the chemical composition of ancient lakes. Three methods are used to estimate paleoalkalinity. Diatoms, molluscs, and fish have certain metabolic requirements that are dependent on pH, alkalinity, or calcium levels; thus fauna and flora can be used as paleoalkalinity indicators. Exchangeable cations on clay minerals can also be used because the relative concentrations of sodium and calcium in lake waters are related to alkalinity. Absence or presence of certain minerals also can serve as a paleoalkalinity indicator. Although the latter two techniques give estimates of paleoalkalinity that are averaged over several hundred or thousand years, their estimates agree with the instantaneous estimates based on biologic considerations. This study shows that the earliest lake phase was very fresh and contained until the end of the Kubi Algi Formation. The Lower Member of the Koobi Fora Formation is shown to have been a fresh- to brackish-water lake. From the beginning of Upper Member time (about 1.8 MY ago) to the present, the lake occupying the Turkana Depression has varied from a brackish lake that overflowed to a closed basin lake that fell below overflow level and whose alkalinity rose to about 200 meq/l.

  2. Comparative study of adipose-derived stem cells and bone marrow-derived stem cells in similar microenvironmental conditions

    Energy Technology Data Exchange (ETDEWEB)

    Guneta, Vipra [Division of Materials Technology, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798 (Singapore); Tan, Nguan Soon [School of Biological Science, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551 (Singapore); KK Research Centre, KK Women' s and Children Hospital, 100 Bukit Timah Road, Singapore 229899 (Singapore); Institute of Molecular and Cell Biology, Agency for Science Technology & Research - A*STAR, 61 Biopolis Drive, Proteos, Singapore 138673 (Singapore); Chan, Soon Kiat Jeremy [School of Biological Science, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551 (Singapore); Tanavde, Vivek [Bioinformatics Institute, Agency for Science Technology & Research - A*STAR, 30 Biopolis Street, Matrix, Singapore 138671 (Singapore); Lim, Thiam Chye [Division of Plastic, Reconstructive and Aesthetic Surgery, Department of Surgery, National University Hospital (NUH) and National University of Singapore (NUS), Kent Ridge Wing, Singapore 119074 (Singapore); Wong, Thien Chong Marcus [Plastic, Reconstructive and Aesthetic Surgery Section, Tan Tock Seng Hospital (TTSH), 11, Jalan Tan Tock Seng, Singapore 308433 (Singapore); Choong, Cleo, E-mail: cleochoong@ntu.edu.sg [Division of Materials Technology, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798 (Singapore); KK Research Centre, KK Women' s and Children Hospital, 100 Bukit Timah Road, Singapore 229899 (Singapore)

    2016-11-01

    Mesenchymal stem cells (MSCs), which were first isolated from the bone marrow, are now being extracted from various other tissues in the body, including the adipose tissue. The current study presents systematic evidence of how the adipose tissue-derived stem cells (ASCs) and bone marrow-derived mesenchymal stem cells (Bm-MSCs) behave when cultured in specific pro-adipogenic microenvironments. The cells were first characterized and identified as MSCs in terms of their morphology, phenotypic expression, self-renewal capabilities and multi-lineage potential. Subsequently, the proliferation and gene expression profiles of the cell populations cultured on two-dimensional (2D) adipose tissue extracellular matrix (ECM)-coated tissue culture plastic (TCP) and in three-dimensional (3D) AlgiMatrix® microenvironments were analyzed. Overall, it was found that adipogenesis was triggered in both cell populations due to the presence of adipose tissue ECM. However, in 3D microenvironments, ASCs and Bm-MSCs were predisposed to the adipogenic and osteogenic lineages respectively. Overall, findings from this study will contribute to ongoing efforts in adipose tissue engineering as well as provide new insights into the role of the ECM and cues provided by the immediate microenvironment for stem cell differentiation. - Highlights: • Native adipose tissue ECM coated on 2D TCP triggers adipogenesis in both ASCs and Bm-MSCs. • A 3D microenvironment with similar stiffness to adipose tissue induces adipogenic differentiation of ASCs. • ASCs cultured in 3D alginate scaffolds exhibit predisposition to adipogenesis. • Bm-MSCs cultured in 3D alginate scaffolds exhibit predisposition to osteogenesis. • The native microenvironment of the cells affects their differentiation behaviour in vitro.

  3. Evaluation of outgassing, tear strength, and detail reproduction in alginate substitute materials.

    Science.gov (United States)

    Baxter, R T; Lawson, N C; Cakir, D; Beck, P; Ramp, L C; Burgess, J O

    2012-01-01

    To compare three alginate substitute materials to an alginate impression material for cast surface porosity (outgassing), tear strength, and detail reproduction. Detail reproduction tests were performed following American National Standards Institute/American Dental Association (ANSI/ADA) Specification No. 19. To measure tear strength, 12 samples of each material were made using a split mold, placed in a water bath until testing, and loaded in tension until failure at a rate of 500 mm/min using a universal testing machine. For cast surface porosity testing, five impressions of a Teflon mold with each material were placed in a water bath (37.8°C) for the in-mouth setting time and poured with vacuum-mixed Silky Rock die stone at 5, 10, 30, and 60 minutes from the start of mixing. The gypsum samples were analyzed with a digital microscope for surface porosity indicative of hydrogen gas release by comparing the surface obtained at each interval with four casts representing no, little, some, and significant porosity. Data analysis was performed using parametric and Kruskal-Wallis analysis of variance (ANOVA), Tukey/Kramer post-hoc tests (α=0.05), and individual Mann-Whitney U tests (α=0.0167). All alginate substitute materials passed the detail reproduction test. Tear strength of the alginate substitute materials was significantly better than alginate and formed three statistically different groups: AlgiNot had the lowest tear strength, Algin-X Ultra had the highest tear strength, and Position Penta Quick had intermediate tear strength. Significant variation in outgassing existed between materials and pouring times (palginate substitute materials exhibited the least outgassing and cast porosity 60 minutes after mixing. Detail reproduction and tear strength of alginate substitute materials were superior to traditional alginate. The outgassing effect was minimal for most materials tested. Alginate substitute materials are superior replacements for irreversible

  4. LANGUAGE OF LOVE IN MARCELIJUS MARTINAITIS "ATMINTYS"

    Directory of Open Access Journals (Sweden)

    Kristina Bačiulienė

    2014-10-01

    Full Text Available The notion of transcendental love in the context of “the eternal return”, the motif of Eros, and the image of a woman are discussed in Marcelijus Martinaitis’s book "Atmintys: meilės lyrikos albumas" (eng. "Reminiscences: the album of love lyrics" (2008. The methods of mythopoetic thought, comparativistics, and interpretative mind have been applied. On the basis of the Bible, mythology, the notion of and relation between Eros and Love, also combining notions developed by philosophers as Nikolai Berdyaev, Erich Fromm, Vladimir Solovyev, phenomenologists as Algis Mickūnas, Mircea Eliade, Plato, and insights by popes Paul VI and Benedict XVI, a concept which might be useful in deciphering the language of love in Marcelijus Martinaitis’s book is introduced. The primary code of the album of love is the concept of memory and the notion of love as seen by Oskaras Milašius, while the secondary code relates to Dante, chivalric romance of Provence troubadours, legends of King Arthur, Celtic mythology. The genre of M. Martinaitis’s reminiscences was born when the poet was studying in Gervinės, and the first collection of Reminiscences appeared in 1986. The text of Reminiscences was seen as hermetic, intertextual, reflecting the act of cosmogony and antropogony in permanent space-time. The process of word re-creation is associated with archetypal consciousness, and the cycle of “the eternal return” prevails. Love is perceived as transcendental and existential basis, a purpose to merge with God, God’s gift to humanity, and the vision of love object. Eros (passion is ambivalent: it is earthly / heavenly, sinful / divine, creating / destructive, and driving power of creation. A woman is seen as earthly / sensual, divine / unreachable. An issue which motif (Christian or courtesan literature dominates in the album of love lyrics is discussed. Album is of great interest as an immanent text.

  5. Comparative study of adipose-derived stem cells and bone marrow-derived stem cells in similar microenvironmental conditions

    International Nuclear Information System (INIS)

    Guneta, Vipra; Tan, Nguan Soon; Chan, Soon Kiat Jeremy; Tanavde, Vivek; Lim, Thiam Chye; Wong, Thien Chong Marcus; Choong, Cleo

    2016-01-01

    Mesenchymal stem cells (MSCs), which were first isolated from the bone marrow, are now being extracted from various other tissues in the body, including the adipose tissue. The current study presents systematic evidence of how the adipose tissue-derived stem cells (ASCs) and bone marrow-derived mesenchymal stem cells (Bm-MSCs) behave when cultured in specific pro-adipogenic microenvironments. The cells were first characterized and identified as MSCs in terms of their morphology, phenotypic expression, self-renewal capabilities and multi-lineage potential. Subsequently, the proliferation and gene expression profiles of the cell populations cultured on two-dimensional (2D) adipose tissue extracellular matrix (ECM)-coated tissue culture plastic (TCP) and in three-dimensional (3D) AlgiMatrix® microenvironments were analyzed. Overall, it was found that adipogenesis was triggered in both cell populations due to the presence of adipose tissue ECM. However, in 3D microenvironments, ASCs and Bm-MSCs were predisposed to the adipogenic and osteogenic lineages respectively. Overall, findings from this study will contribute to ongoing efforts in adipose tissue engineering as well as provide new insights into the role of the ECM and cues provided by the immediate microenvironment for stem cell differentiation. - Highlights: • Native adipose tissue ECM coated on 2D TCP triggers adipogenesis in both ASCs and Bm-MSCs. • A 3D microenvironment with similar stiffness to adipose tissue induces adipogenic differentiation of ASCs. • ASCs cultured in 3D alginate scaffolds exhibit predisposition to adipogenesis. • Bm-MSCs cultured in 3D alginate scaffolds exhibit predisposition to osteogenesis. • The native microenvironment of the cells affects their differentiation behaviour in vitro.

  6. Basic investigation of the laminated alginate impression technique: Setting time, permanent deformation, elastic deformation, consistency, and tensile bond strength tests.

    Science.gov (United States)

    Kitamura, Aya; Kawai, Yasuhiko

    2015-01-01

    Laminated alginate impression for edentulous is simple and time efficient compared to border molding technique. The purpose of this study was to examine clinical applicability of the laminated alginate impression, by measuring the effects of different Water/Powder (W/P) and mixing methods, and different bonding methods in the secondary impression of alginate impression. Three W/P: manufacturer-designated mixing water amount (standard), 1.5-fold (1.5×) and 1.75-fold (1.75×) water amount were mixed by manual and automatic mixing methods. Initial and complete setting time, permanent and elastic deformation, and consistency of the secondary impression were investigated (n=10). Additionally, tensile bond strength between the primary and secondary impression were measured in the following surface treatment; air blow only (A), surface baking (B), and alginate impression material bonding agent (ALGI-BOND: AB) (n=12). Initial setting times significantly shortened with automatic mixing for all W/P (p<0.05). The permanent deformation decreased and elastic deformation increased as high W/P, regardless of the mixing method. Elastic deformation significantly reduced in 1.5× and 1.75× with automatic mixing (p<0.05). All of these properties resulted within JIS standards. For all W/P, AB showed a significantly high bonding strength as compared to A and B (p<0.01). The increase of mixing water, 1.5× and 1.75×, resulted within JIS standards in setting time, suggesting its applicability in clinical setting. The use of automatic mixing device decreased elastic strain and shortening of the curing time. For the secondary impression application of adhesives on the primary impression gives secure adhesion. Copyright © 2014 Japan Prosthodontic Society. Published by Elsevier Ltd. All rights reserved.

  7. Isolation and characterization of Wharton’s jelly-derived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined serum-free three-dimensional system

    Directory of Open Access Journals (Sweden)

    Cardoso Tereza C

    2012-05-01

    bovine-derived UC-WJ cells was included in the culture which demonstrated the immunossupression profile typically observed among isolated mesenchymal cells from other species. After classified the UC-WJ cells as mesenchymal stromal phenotype the in vitro 3D cultures was performed using the AlgiMatrix® protocol. Based on the size of spheroids (283,07 μm ± 43,10 μm we found that three weeks of culture was the best period to growth the UC-WJ cells on 3D dimension. The initial cell density was measured and the best value was 1.5 × 106 cells/well. Conclusions We described for the first time the isolation and characterization of UC-WJ cells in a serum-free condition and maintenance of primitive mesenchymal phenotype. The culture was stable under 60 consecutive passages with no genetic abnormalities and proliferating ratios. Taken together all results, it was possible to demonstrate an easy way to isolate and culture of bovine-derived UC-WJ cells under 2D and 3D serum-free condition, from fetal adnexa with a great potential in cell therapy and biotechnology.

  8. Editorial. Dialogue, Communication and Collaboration: Aspects of Philosophy and Communication

    Directory of Open Access Journals (Sweden)

    Jovilė Barevičiūtė

    2016-03-01

    versus Antirealism in Science Education is a contribution by Seungbae Park, in which he attempts to define how the dialogue between teachers and students is possible, as he takes the position stating that the doctrine of scientific realism is much more effective than provided opportunities of scientific antirealism. And finally, Algis Mickūnas, in his article The Different Other and Dialogue, discusses the reasons why members of different communities find it difficult to establish dialogue-based relationships and why in some cases they remain imprisoned in the state of a monologue. This issue of the journal presents a truly wide field of investigations into opportunities and obstacles for communication, interaction and collaboration. It is pleasing to see that representatives of various humanities and social sciences joined the same dialogue. Looking forward to the productive insights in the future, the Editor would like to express her gratitude to the authors of this issue.

  9. Exploring Unconventional Hydrocarbons in the Makó Trough, Pannonian basin, Hungary: Results and Challenges

    Science.gov (United States)

    Horvath, Anita; Bada, Gabor; Szafian, Peter; Sztano, Orsolya; Law, Ben; Wallis, Rod

    2010-05-01

    subsidence (Endrőd Formation). The basin was then almost completely filled by the turbidites of a prograding delta system (Szolnok Formation), followed by the sediments from the pro-delta (Algyő Formation) and the delta plain (Újfalu Formation). During the Plio- and Pleistocene, the area continued to subside at a decreased rate, providing a limited accumulation space filled by a thick sequence of alluvial deposits similar to the paleo-environment of the Tisza River and its tributaries prior to regulation. Our modeling of basin evolution in terms of subsidence, thermal and maturation history based on the results of geochemical, petrophysical, and paleontological investigations reveals that the organic matter accumulated in the trough underwent exceptionally rapid maturation during the last 5 to 7 million years. In fact, source rocks continue to mature at present time, and the hydrocarbons they generate continue to migrate and trap. The rather young age of the Pannonian Basin and its hydrocarbon system makes it quite different from the other examples of basin-centered gas accumulation, such as the classic Rocky Mountains in the U.S., or the Karoo Basin in South Africa, the Beetaloo Basin in Australia, making up the exploration portfolio of Falcon.

  10. Editorial. Kultūros reiškiniai šiuolaikinių informacijos ir komunikacijos technologijų sąlygomis: nuo meno ir televizijos iki politikos

    Directory of Open Access Journals (Sweden)

    Jovilė Barevičiūtė

    2015-10-01

    Full Text Available Šiame žurnalo numeryje aptariami kai kurie daugiaaspekčiai šiuolaikinės kultūros reiškiniai, apimant tokius jų, kaip menas, televizija, politika ir kt. Medijos ir komunikacija nūdien intensyviai skverbiasi kone į visas darbo ir laisvalaikio veiklos, viešojo ir privataus sektorių sritis, reprezentuodamos dinamišką ir netolygią gyvenamojo pasaulio tikrovę. Medijuotoji komunikacija vis labiau tampa dominuojančia socializacijos forma, ji išstumia gyvąją komunikaciją: kuo toliau, tuo labiau šis reiškinys nusako ne tik jaunimo, bet ir brandaus amžiaus žmonių šiandienį bendravimo būdą. Tad akivaizdu, kad XXI a. žmogus vis labiau pasineria į įvairių medijų tinklus, apraizgančius jo gyvenimą ir tampančius pagrindine socialios ir visavertės eg zistencijos sąlyga. Tokios socialinės institucijos, kaip menas, televizija, politika, religija, šeima ir daugelis kitų, nūdien jau nebėra tokios savarankiškos, kaip, tarkime, prieš dešimt, dvidešimt ar daugiau metų. Jų suverenitetą daugiausia transformuoja būtent medijos, šioms institucijoms diktuojančios savas sąlygas ir primetančios savas taisykles. Kitaip tariant, medijos tampa tokios galingos, kad ima steigti savuosius dėsnius, kuriems vis labiau paklūsta tradicinė gyvosios socializacijos aplinka. Taip kyla daugybė diskutuotinų ir ginčytinų klausimų, paliečiančių socialinių institucijų apibrėžties, autonomijos ir suvereniteto aspektus. Šiuos aspektus iš įvairių perspektyvų gvildena ir šio numerio autoriai. Eugenija Krukauskienė ir Viktorija Žilinskaitė-Vytienė savo straipsnyje aptaria kultūros vartojimo klausimus tirdamos, kaip šiuolaikinio lietuviškojo kino meno pavyzdžius suvokia ir vertina tam tikrų amžiaus kategorijų jaunimas. Algis Mickūnas gilinasi į filosofinius šiuolaikinių diskursų klausimus, pasirinkdamas kūniškumo, lytėjimo ir taktilikos aspektus, glaudžiai susijusius su medijuotosios komunikacijos tema