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Sample records for atr kartini berdasarkan

  1. ANALISIS KESELAMATAN TERMOHIDROLIK BULK SHIELDING REAKTOR KARTINI

    Directory of Open Access Journals (Sweden)

    Azizul Khakim

    2015-10-01

    Full Text Available ABSTRAK ANALISIS KESELAMATAN TERMOHIDROLIK BULK SHIELDING REAKTOR KARTINI. Bulk shielding merupakan fasilitas yang terintegrasi dengan reaktor Kartini yang berfungsi sebagai penyimpanan sementara bahan bakar bekas. Fasilitas ini merupakan fasilitas yang termasuk dalam struktur, sistem dan komponen (SSK yang penting bagi keselamatan. Salah satu fungsi keselamatan dari sistem penanganan dan penyimpanan bahan bakar adalah mencegah kecelakaan kekritisan yang tak terkendali dan membatasi naiknya temperatur bahan bakar. Analisis keselamatan paling kurang harus mencakup analisis keselamatan dari sisi neutronik dan termo hidrolik Bulk shielding. Analisis termo hidrolik ditujukan untuk memastikan perpindahan panas dan proses pendinginan bahan bakar bekas berjalan baik dan tidak terjadi akumulasi panas yang mengancam integritas bahan bakar. Code tervalidasi PARET/ANL digunakan untuk analisis pendinginan dengan mode konveksi alam. Hasil perhitungan menunjukkan bahwa mode pendinginan konvekasi alam cukup memadai dalam mendinginkan panas sisa tanpa mengakibatkan kenaikan temperatur bahan bakar yang signifikan. Kata kunci: Bulk shielding, bahan bakar bekas, konveksi alam, PARET.   ABSTRACT THERMAL HYDRAULIC SAFETY ANALYSIS OF BULK SHIELDING KARTINI REACTOR. Bulk shielding is an integrated facility to Kartini reactor which is used for temporary spent fuels storage. The facility is one of the structures, systems and components (SSCs important to safety. Among the safety functions of fuel handling and storage are to prevent any uncontrolable criticality accidents and to limit the fuel temperature increase. Safety analyses should, at least, cover neutronic and thermal hydraulic calculations of the bulk shielding. Thermal hydraulic analyses were intended to ensure that heat removal and the process of the spent fuels cooling takes place adequately and no heat accumulation that challenges the fuel integrity. Validated code, PARET/ANL was used for analysing the

  2. The current status of Kartini research reactor

    Energy Technology Data Exchange (ETDEWEB)

    Tjiptono, Tri Wulan; Syarip,

    1998-10-01

    The Kartini reactor reached the first criticality on January 25, 1979. In the first three years, the reactor power is limited up to 50 kW thermal power and on July 1, 1982 has been increased to 100 kW. It has been used as experiments facility by researcher of Atomic Energy National Agency and students of the Universities. Three beam tubes used as experiments facilities, the first, is used as a neutron source for H{sub 2}O-Natural Uranium Subcritical Assembly, the second, is developed for neutron radiography facility and the third, is used for gamma radiography facility. The other facilities are rotary rack and two pneumatic transfer systems, one for delayed neutron counting system and the other for the new Neutron Activation Analysis (NAA) facility. The rotary rack used for isotope production for NAA purpose (for long time irradiation), the delayed neutron counting system used for analysis the Uranium contents of the ores and the new NAA is provided for short live elements analysis. In the last three years the Reactor Division has a joint use program with the Nuclear Component and Engineering Center in research reactor instrumentation and control development. (author)

  3. Design of Kartini reactor radiation monitor system using lab view

    International Nuclear Information System (INIS)

    Adi Abimanyu; Jumari; Achmad Fahrul Aji; Muhammad Khoiri

    2014-01-01

    Kartini Reactor operation will result in radiation exposure. Gamma radiation exposure rate at the Kartini Reactor monitored by several radiation monitors (Ludlum) that integrate with the computer, so that the rate of radiation exposure is always monitored. Current monitoring system combines six radiation monitor in one computer monitor radiation, and monitoring performed by operators and supervisors to see how the radiation exposure rate measured in the area around the reactor core in a periodic time manually. This research will develop a system to monitor radiation exposure in Kartini reactor based ATMega8 micro controller for interface between radiation monitor and computer and also Graphical User Interface (GUI) develop using Lab view software that makes monitoring is easier and documented regularly. This system is testing by simulation, it is done by replacing the function of the radiation monitoring devices (Ludlum) in Kartini Reactor with computers that send serial data with the same format with a format that is sent by Ludlum. The results show that the interface system has the ability to operate in a range of baud rate 1,200 bps, 2,400 bps, 4,800 bps, 9,600 bps, 14,400 bps, 19,200 bps and 38,400 bps, with the ability to provide realtime information every 6 seconds and able to document the rate of exposure to radiation in the form of logbook. (author)

  4. Safety Evaluation of Kartini Reactor Based on Instrumentation System Design

    International Nuclear Information System (INIS)

    Tjipta Suhaemi; Djen Djen Dj; Itjeu K; Johnny S; Setyono

    2003-01-01

    The safety of Kartini reactor has been evaluated based on instrumentation system aspect. The Kartini reactor is designed by BATAN. Design power of the reactor is 250 kW, but it is currently operated at 100 kW. Instrumentation and control system function is to monitor and control the reactor operation. Instrumentation and control system consists of safety system, start-up and automatic power control, and process information system. The linear power channel and logarithmic power channel are used for measuring power. There are 3 types of control rod for controlling the power, i.e. safety rod, shim rod, and regulating rod. The trip and interlock system are used for safety. There are instrumentation equipment used for measuring radiation exposure, flow rate, temperature and conductivity of fluid The system of Kartini reactor has been developed by introducing a process information system, start-up system, and automatic power control. It is concluded that the instrumentation of Kartini reactor has followed the requirement and standard of IAEA. (author)

  5. Safety culture and quality management of Kartini research reactor

    Energy Technology Data Exchange (ETDEWEB)

    Syarip [Yogyakarta Nuclear Research Centre, Yogyakarta (Indonesia); Hauptmanns, Ulrich [Department of Plant Design and Safety, Otto-Von-Guericke-University, Magdeburg (Germany)

    1999-10-01

    The evaluation for assessing the safety culture and quality of safety management of Kartini research reactor is presented. The method is based on the concept of management control of safety (audit) as well as by using the developed method i.e. the questionnaires concerning areas of relevance which have to be answered with value statements. There are seven statements or qualifiers in answering the questions. Since such statements are vague, they are represented by fuzzy numbers. The weaknesses can be identified from the different areas contemplated. The evaluation result show that the quality of safety management of Kartini research reactor is globally rated as 'Average'. The operator behavior in the implementation of 'safety culture' concept is found as a weakness, therefore this area should be improved. (author)

  6. Safety culture and quality management of Kartini research reactor

    International Nuclear Information System (INIS)

    Syarip; Hauptmanns, Ulrich

    1999-01-01

    The evaluation for assessing the safety culture and quality of safety management of Kartini research reactor is presented. The method is based on the concept of management control of safety (audit) as well as by using the developed method i.e. the questionnaires concerning areas of relevance which have to be answered with value statements. There are seven statements or qualifiers in answering the questions. Since such statements are vague, they are represented by fuzzy numbers. The weaknesses can be identified from the different areas contemplated. The evaluation result show that the quality of safety management of Kartini research reactor is globally rated as 'Average'. The operator behavior in the implementation of 'safety culture' concept is found as a weakness, therefore this area should be improved. (author)

  7. Design Engineering of Safety Parameter Display for Kartini Reactor

    International Nuclear Information System (INIS)

    Yoyok Dwi Setyo Pambudi; Suharyo Widagdo; Aliq Zuhdi; Darlis

    2003-01-01

    Modification information parameter of Reactor Kartini has been conducted. This program use compiler LabView 5.0 that compatible and relevant with National Instrument card. These card mostly use in reactor instrumentation and industry. The information that been display is control rod position, fuel temperature, and water pH. The development in this research is control rod figure that can move up and down as the output from reactor power. Beside this is a module-module security figure that can light if there is a warning signal. Maximum high of control rod is 100 cm. (author)

  8. Kartini Research Reactor prospective studies for neutron scattering application

    International Nuclear Information System (INIS)

    Widarto

    1999-01-01

    The Kartini Research Reactor (KRR) is located in Yogyakarta Nuclear Research Center, Yogyakarta - Indonesia. The reactor is operated for 100 kW thermal power used for research, experiments and training of nuclear technology. There are 4 beam ports and 1 column thermal are available at the reactor. Those beam ports have thermal neutron flux around 10 7 n/cm 2 s each other and used for sub critical assembly, neutron radiography studies and Neutron Activation Analysis (NAA). Design of neutron collimator has been done for piercing radial beam port and the calculation result of collimated neutron flux is around 10 9 n/cm 2 s. This paper describes experiment facilities and parameters of the Kartini research reactor, and further more the prospective studies for neutron scattering application. The purpose of this paper is to optimize in utilization of the beam ports facilities and enhance the manpower specialty. The special characteristic of the beam ports and preliminary studies, pre activities regarding with neutron scattering studies for KKR is presented. (author)

  9. The Calculation Of Total Radioactivity Of Kartini Reactor Fuel Element

    International Nuclear Information System (INIS)

    Budisantoso, Edi Trijono; Sardjono, Y.

    1996-01-01

    The total radioactivity of Kartini reactor fuel element has been calculated by using ORIGEN2. In this case, the total radioactivity is the sum of alpha, beta, and gamma radioactivity from activation products nuclides, actinide nuclides and fission products nuclides in the fuel element. The calculation was based on irradiation history of fuel in the reactor core. The fuel element no 3203 has location history at D, E, and F core zone. The result is expressed in graphics form of total radioactivity and photon radiations as function of irradiation time and decay time. It can be concluded that the Kartini reactor fuel element in zone D, E, and F has total radioactivity range from 10 Curie to 3000 Curie. This range is for radioactivity after decaying for 84 days and that after reactor shut down. This radioactivity is happened in the fuel element for every reactor operation and decayed until the fuel burn up reach 39.31 MWh. The total radioactivity emitted photon at the power of 0.02 Watt until 10 Watt

  10. ATR performance modeling concepts

    Science.gov (United States)

    Ross, Timothy D.; Baker, Hyatt B.; Nolan, Adam R.; McGinnis, Ryan E.; Paulson, Christopher R.

    2016-05-01

    Performance models are needed for automatic target recognition (ATR) development and use. ATRs consume sensor data and produce decisions about the scene observed. ATR performance models (APMs) on the other hand consume operating conditions (OCs) and produce probabilities about what the ATR will produce. APMs are needed for many modeling roles of many kinds of ATRs (each with different sensing modality and exploitation functionality combinations); moreover, there are different approaches to constructing the APMs. Therefore, although many APMs have been developed, there is rarely one that fits a particular need. Clarified APM concepts may allow us to recognize new uses of existing APMs and identify new APM technologies and components that better support coverage of the needed APMs. The concepts begin with thinking of ATRs as mapping OCs of the real scene (including the sensor data) to reports. An APM is then a mapping from explicit quantized OCs (represented with less resolution than the real OCs) and latent OC distributions to report distributions. The roles of APMs can be distinguished by the explicit OCs they consume. APMs used in simulations consume the true state that the ATR is attempting to report. APMs used online with the exploitation consume the sensor signal and derivatives, such as match scores. APMs used in sensor management consume neither of those, but estimate performance from other OCs. This paper will summarize the major building blocks for APMs, including knowledge sources, OC models, look-up tables, analytical and learned mappings, and tools for signal synthesis and exploitation.

  11. PEMODELAN KOLIMATOR DI RADIAL BEAM PORT REAKTOR KARTINI UNTUK BORON NEUTRON CAPTURE THERAPY

    Directory of Open Access Journals (Sweden)

    Bemby Yulio Vallenry

    2015-03-01

    Full Text Available Salah satu metode terapi kanker adalah Boron Neutron Capture Therapy (BNCT. BNCT memanfaatkan tangkapan neutron oleh 10B yang terendapkan pada sel kanker. Keunggulan BNCT dibandingkan dengan terapi radiasi lainnya adalah tingkat selektivitas yang tinggi karena tingkatannya adalah sel. Pada penelitian ini dilakukan pemodelan kolimator di radial beamport reaktor Kartini sebagai dasar pemilihan material dan manufature kolimator sebagai sumber neutron untuk BNCT. Pemodelan ini dilakukan dengan simulasi menggunakan perangkat lunak Monte Carlo N-Particle versi 5 (MCNP 5. MCNP 5 adalah suatu paket program untuk memodelkan sekaligus menghitung masalah transpor partikel dengan mengikuti sejarah hidup neutron semenjak lahir, bertranspor pada bahan hingga akhirnya hilang karena mengalami reaksi penyerapan atau keluar dari sistem. Pemodelan ini menggunakan variasi material dan ukurannya agar menghasilkan nilai dari tiap parameter-parameter yang sesuai dengan rekomendasi I International Atomic Energy Agency (IAEA untuk BNCT, yaitu fluks neutron epitermal (Фepi > 9 n.cm-2.s-1, rasio antara laju dosis neutron cepat dan fluks neutron epitermal (Ḋf/Фepi 0,7. Berdasarkan hasil optimasi dari pemodelan ini, material dan ukuran penyusun kolimator yang didapatkan yaitu 0,75 cm Ni sebagai dinding kolimator, 22 cm Al sebagai moderator dan 4,5 cm Bi sebagai perisai gamma. Keluaran berkas radiasi yang dihasilkan dari pemodelan kolimator radial beamport yaitu Фepi = 5,25 x 106 n.cm-2s-1, Ḋf/Фepi =1,17 x 10-13 Gy.cm2.n-1, Ḋγ/Фepi = 1,70 x 10-12 Gy.cm2.n-1, Фth/Фepi = 1,51 dan J/Фepi = 0,731. Berdasarkan penelitian ini, hasil optimasi 5 parameter sebagai persyaratan kolimator untuk BNCT yang keluar dari radial beam port tidak sepenuhnya memenuhi kriteria yang direkomendasikan oleh IAEA sehingga perlu dilakukan penelitian lebih lanjut agar tercapainya persyaratan IAEA. Kata kunci: BNCT, radial beamport, MCNP 5, kolimator   One of the cancer therapy methods is

  12. The Expert System For Safety Assesment Of Kartini Reactor Operation And Maintenance

    International Nuclear Information System (INIS)

    Syarip

    2000-01-01

    An expert system for safety assessment of Kartini reactor operation and maintenance based on fuzzy logic method has been made. The expert system is developed from the Fuzzy Expert System Tools (FEST), i.e. by developing the knowledge base and data base files of Kartini research reactor system and operations with an inference engine based on FEST. The knowledge base is represented in the procedural knowledge as heuristic rules or generally known as rule-base in the from of If-then rule. The fuzzy inference process and the conclusion of the rule is done by FEST based on direct chaining method with interactive as well as non-interactive modes. The safety assessment of Kartini reactor based on this method gives more realistic value than the conventional method or binary logic

  13. Preliminary studi on neutronic aspect of a conceptual design of the Kartini reactor base ADS facility

    International Nuclear Information System (INIS)

    Tegas Sutondo

    2012-01-01

    A preliminary study on neutronic aspect of a conceptual design of ADS facility with the basis of Kartini Reaktor, has been performed. The study was intended to see the feasibility from neutronic point of view of Kartini reactor, to be used as a small scale of NPP’s waste transmutation experimental facility. A SRAC code was used as the basis of calculations. The results indicate that the presence of minor actinides (MA) will give a positive reactivity, which tends to increase with the increase of MA concentrations. Based on the defined criteria of subcriticality and by considering the core power distributions and the level of reactivity contribution of MA element, it is concluded that Kartini reactor is potential enough to be used as an ADS experimental facility, mainly for MA concentration between 30 to 50 % of the assumed mixture of C-MA matrix. (author)

  14. Evaluation of environmental radioactivity monitoring data around the Kartini Reactor area

    International Nuclear Information System (INIS)

    Yazid, M; Sutrisno; Sukarman-Aminjoyo; Zaenal-Abidin

    1996-01-01

    Evaluation of environmental radioactivity monitoring data around the Kartini Reactor area has been done. The aim of this investigation is for tracing the possibility of radioactivity released in the environment during the operation of Kartini reactor. The data was evaluated were monthly monitored data taken from 1986 to 1994 period. The method of analysis was done by comparing the environmental radioactivity data before and after reactor commissioning, off side the reactor up to a radius of 5.000 meters and more than 5.000 meters from Kartini reactor and also compared to the maximum permissible radioactivity according to the current regulation. This evaluation showed that there was no indication of radioactivity release to the environment during this period of reactor operation

  15. REPRESENTASI NILAI-NILAI AL QURAN DALAM NOVEL KARTINI KARYA ABIDAH EL KHALIEQY

    Directory of Open Access Journals (Sweden)

    Haniah Haniah

    2018-04-01

    Full Text Available Penelitian ini bertujuan mengkaji nilai-nilai al quran yang ada di dalam novel Kartini karya Abidah El Khalieqy. Pengkajian ini didasari karena terdegradasinya moral generasi saat ini. Penurunan kualitas moral tersebut dilatarbelakangi oleh lemahnya keimanan di dalam diri seiring arus globalisasi yang tidak terbendung lagi. Penelitian ini adalah penelitian kualitatif. Peneliti akan mendeskripsikan hasil penelitian dengan rinci. Sumber data dari penelitian ini adalah novel Kartini karya Abidah El Khalieqy, sedangkan datanya adalah kutipan-kutipan teks di dalam novel yang mencerinkan nilai-nilai al quran.  Teknik analisis data yang digunakan adalah teknik analisis interaktif yang dikembangkan oleh Miles dan Huberman. Hasil penelitian ini menunjukan bahwa nilai-nilai al quran yang tercermin di dalam novel Kartini di antaranya kewajiban membaca, mencintai al quran, berbakti kepada orang tua, berserah diri kepada Allah, beribadah sebaik-baiknya, dan keutamaan menjadi orang yang berilmu.

  16. Atlantic Test Range (ATR)

    Data.gov (United States)

    Federal Laboratory Consortium — ATR controls fully-instrumented and integrated test ranges that provide full-service support for cradle-to-grave testing. Airspace and surface target areas are used...

  17. Gamma Radiation Assessment In Kartini Reactor And Its Vicinity

    International Nuclear Information System (INIS)

    Yazid, M.; Supriyatni, E.; Maryono; Bastianudin, Aris

    2000-01-01

    Measurement to calculate dose assesment for gamma radiation in Kartini Reactor and its vicinity has been done whether on operated or un operated condition. Measurement was performed using height pressured ionization chamber, Reuther Stokes RS-112 production. Measurement location was determined based on distance variation inwardly and outwardly of reactor building and its vicinity. The result showed that the average dose rate in the reactor building when un operated is in the range of 11.4-38.6 mu rad/hour and when the reactor operated is 166.4-1910.9 mu rad/hour. While the vicinity of the reactor on operated condition the average dose rate is 34.4-38.6 mu rad/hour in un operated condition is 6.9-7.0 mu rad/hour. This result showed that the reactor operated did not rise the radiation exposure level in its vicinity. From the personnel assesment dose rate of gamma radiation is 28.54 mrem/week on operated condition, 0.90 mrem.week on un operated condition. While dose rate outside the reactor is 0.44 and 0.27 mrem/week for operated and un operated condition consecutively. This dose rate is still below maximum permissible dose than recommended by the national regulation of radiation protection from BAPETEN No. 01/Ka.BAPETEN/V-99

  18. Kartini's Children: On the Need for Thinking Gender and Education Together on a World Scale

    Science.gov (United States)

    Connell, Raewyn

    2010-01-01

    A world policy agenda for gender equality in education now exists, realising the idea of earlier reformers such as Kartini. This agenda, however, makes assumptions that are strongly contested by research and policy debates in national forums. This essay urges shifting the framework of gender analysis to global scale. It outlines what is involved…

  19. ATR neutron spectral characterization

    Energy Technology Data Exchange (ETDEWEB)

    Rogers, J.W.; Anderl, R.A.

    1995-11-01

    The Advanced Test Reactor (ATR) at INEL provides intense neutron fields for irradiation-effects testing of reactor material samples, for production of radionuclides used in industrial and medical applications, and for scientific research. Characterization of the neutron environments in the irradiation locations of the ATR has been done by means of neutronics calculations and by means of neutron dosimetry based on the use of neutron activation monitors that are placed in the various irradiation locations. The primary purpose of this report is to present the results of an extensive characterization of several ATR irradiation locations based on neutron dosimetry measurements and on least-squares-adjustment analyses that utilize both neutron dosimetry measurements and neutronics calculations. This report builds upon the previous publications, especially the reference 4 paper. Section 2 provides a brief description of the ATR and it tabulates neutron spectral information for typical irradiation locations, as derived from the more historical neutron dosimetry measurements. Relevant details that pertain to the multigroup neutron spectral characterization are covered in section 3. This discussion includes a presentation on the dosimeter irradiation and analyses and a development of the least-squares adjustment methodology, along with a summary of the results of these analyses. Spectrum-averaged cross sections for neutron monitoring and for displacement-damage prediction in Fe, Cr, and Ni are given in section 4. In addition, section4 includes estimates of damage generation rates for these materials in selected ATR irradiation locations. In section 5, the authors present a brief discussion of the most significant conclusions of this work and comment on its relevance to the present ATR core configuration. Finally, detailed numerical and graphical results for the spectrum-characterization analyses in each irradiation location are provided in the Appendix.

  20. ATR confinement leakage determination

    International Nuclear Information System (INIS)

    Kuan, P.; Buescher, B.J.

    1998-01-01

    The air leakage rate from the Advanced Test Reactor (ATR) confinement is an important parameter in estimating hypothesized accidental releases of radiation to the environment. The leakage rate must be determined periodically to assure that the confinement has not degraded with time and such determination is one of the technical safety requirements of ATR operation. This paper reviews the methods of confinement leakage determination and presents an analysis of leakage determination under windy conditions, which can complicate the interpretation of the determined leakage rates. The paper also presents results of analyses of building air exchange under windy conditions. High wind can enhance air exchange and this could increase the release rates of radioisotopes following an accident

  1. PUREX new substation ATR

    International Nuclear Information System (INIS)

    Nelson, D.E.

    1997-01-01

    This document is the acceptance test report (ATR) for the New PUREX Main and Minisubstations. It covers the factory and vendor acceptance and commissioning test reports. Reports are presented for the Main 5 kV substation building, the building fire system, switchgear, and vacuum breaker; the minisubstation control building and switch gear; commissioning test; electrical system and loads inspection; electrical utilities transformer and cable; and relay setting changes based on operational experience

  2. Design and Development of Data Acquisition System Process Parameters of Kartini Reactor

    International Nuclear Information System (INIS)

    Prajitno

    2009-01-01

    Design and development of computer program for data acquisition system of process parameters of the Kartini reactor have been done. System was designed using industrial computer which equipped with electronic module PCL-812PG. The function of computer is to take parameter data of reactor process, processing the data and displaying on the numeric form and bar graphic. Electronics module PCL- 12PG was installed in one of computer slot, functions to convert from analog signal to digital, received digital status signal and produce digital output. The analog signal and digital status got from logarithmic power channel, linear power channel dan three control rod. Result of data acquisition is merged in the form of ASCII characters block, send to the master computer serially with communications protocols RS-232. Computer program which has been developed was tested and used for monitoring Kartini reactor operation and give good performance result. (author)

  3. Ageing investigation and upgrading of components/systems of Kartini research reactor

    Energy Technology Data Exchange (ETDEWEB)

    Syarip,; Setiawan, Widi [Yogyakarta Nuclear Research Centre, Yogyakarta (Indonesia)

    1998-10-01

    Kartini research reactor has been operated in good condition and has demonstrated successful operation for the past 18 years, utilized for: reactor kinetic and control studies, instrumentation tests, neutronic and thermohydraulic studies, routine neutron activation analysis, reactor safety studies, training for research reactor operators and supervisors, and reactor physics experiments. Several components of Kartini reactor use components from the abandoned IRT-2000 Project at Serpong and from Bandung Reactor Centre such as: reactor tank, reactor core, heat exchanger, motor blower for ventilation system, fuel elements, etc. To maintain a good operating performance and also for aging investigation purposes, the component failure data collection has been done. The method used is based on the Manual on Reliability Data Collection For Research Reactor PSAs, IAEA TECDOC 636, and analyzed by using Data Entry System (DES) computer code. Analysis result shows that the components/systems failure rate of Kartini reactor is around 1,5.10{sup -4} up to 2,8.10{sup -4} per hour, these values are within the ranges of the values indicated in IAEA TECDOC 478. Whereas from the analysis of irradiation history shows that the neutron fluence of fuel element with highest burn-up (2,05 gram U-235 in average) is around 1.04.10{sup 16} n Cm{sup -2} and this value is still far below its limiting value. Some reactor components/systems have been replaced and upgraded such as heat exchanger, instrumentation and control system (ICS), etc. The new reactor ICS was installed in 1994 which is designed as a distributed structure by using microprocessor based systems and bus system technology. The characteristic and operating performance of the new reactor ICS, as well as the operation history and improvement of the Kartini research reactor is presented. (J.P.N.)

  4. Pengaruh Pemindahan Perpustakaan Umum Kabupaten Rembang ke Lokasi Pariwisata Pantai Kartini Terhadap Minat Kunjung Pemustaka

    Directory of Open Access Journals (Sweden)

    Athiyah Kamaliyah

    2016-03-01

    Full Text Available The aim ofthis research are (1 to know the influence of Rembang Public Library transfer to Kartini Beach tourism area toward animo of visits to the libratyt (2 to know user perception about Rembang Public Library transfer to Kartini Beach tourism area (3 to know animo of visits to Rembang Public Library. This was a quantitative research that used correlation method between location (independent variable and animo of visits (dependent variable. Data were collected through questionnaire, interview and documentation. Data analysis used product moment correlation and processed using SPSS version 17 for Windows. The result of the study showed there was significant correlation between location and animo of visits. Score of correlation was 0.632 at significance level 5% r table is 0.202. The Rembang public library transfer to Kartini Beach tourism area belonged to good category whereby grand mean of location variable was 2.86. Animo of visits also belonged to high category whereby grand mean was 3.01. Key word: location, animo ofvisit, public library

  5. Measurement and evaluation of the external radiation level at reactor Kartini

    International Nuclear Information System (INIS)

    Atok Suhartanto; Suparno

    2013-01-01

    Measurement and evaluation of external radiation level at reactor Kartini in 2012 has been done. The purpose of this activity is to know the external radiation level as a result of the radioactive or radiation source usage, toward the operational of limit condition. The measurement is using survey meter Inspector 11086, factor of calibration 0.991 mR/h, at 9 locations is: Control room area, Thermal column facilities, Demineralizer, Beamport radiography facilities, bulk shielding Deck, Subcritical facilities, Reactor hall, Deck reactor and on the surface of reactor water tank . The highest room average measurement result in 9 working areas for 12 months continuously are at the reactor tank location is between 13.05±1.09 (xlO -2 mSv/hour) to 16.80±1.40 (x10 -2 mSv/hour), and the lowest measurement result in 1 location (control room) is 0.02±0.005 (x10 -2 mSv/hour) to 0.035±0.009 (x10 -2 mSv/hour). The Kartini reactor is involved in the control area which has potentially contaminated and has radiation exposure at the level of 6 mSv/year. Radiation Protection Officer that work in interval will received radiation exposure dosage of 8.4 mSv/year. This dosage is still below the Below Dosage Value which is recommended by, BAPETEN decree No, 4, 2013 about Protection and Radiation Safety in Nuclear Energy Application at 20 mSv/year. The result of the evaluation above shows that the external radiation which occurred in each area is still below the operational of limit condition that is written on the Kartini reactor safety analysis report, on document number: C7/05/B2/LAK/2010, revision 7. So that the workplace is safe for work monitored. (author)

  6. Determination and Analysis of Ar-41 Dose Rate Characteristic at Thermal Column of Kartini Reactor

    International Nuclear Information System (INIS)

    Widarto; Sardjono, Y.

    2007-01-01

    Determination and Analysis of Ar-41 activity dose rate at the thermal column after shutdown of Kartini reactor has been done. Based on evaluation and analysis concluded that external dose rate is D = 1.606x10 -6 Sv/second and internal dose rate is 3.429x10 -1 1 Sv/second. It means that if employee work at the column thermal area for 15 minutes a day, 5 days a week, in a year will be 0.376 Sv still under dose rate limit i.e. 0.5 Sv, so that the column thermal facility is safely area. (author)

  7. Analysis of Radioactivity Contamination Level of Kartini Reactor Efluen Gas to the Environment

    International Nuclear Information System (INIS)

    Suratman; Purwanto; Aminjoyo, S

    1996-01-01

    The analysis of radioactivity contamination level of Kartini reactor efluen gas to the environment has been done from 13-10-'95 until 8-2-'96. The aim of this research is to determine the radioactivity contamination level on the environment resulted from the release of Kartini reactor efluen gas and other facilities at Yogyakarta Nuclear Research Centre through stack. The analysis methods is the student t-test, the first count factor test and the gamma spectrometry. The gas sampling were carried out in the stack reactor, reactor room, environment and in other room for comparison. Efluen gas was sucked through a filter by a high volume vacuum pump. The filter was counted for beta, gamma and alpha activities. The radioactivity contamination level of the efluen gas passing through the stack to the environment was measured between 0.57 - 1.34 Bq/m3, which was equal to the airborne radioactivity in environment between 0.69 - 1.12 Bq/m3. This radioactivity comes from radon daughter, decay products result from the natural uranium and thorium series of the materials of the building

  8. Evaluation of radiological impacts for environmental radioactivity distribution in the Kartini reactor area

    International Nuclear Information System (INIS)

    Yazid, M.; Suratman; Sutresna, G.; Aminjoyo, S.

    1996-01-01

    This evaluation covered of gross radioactivity. K-40 radioactivity in the water, soil and grass samples. The measurement of Cs-137 and Sr-90 radioactivity in the water samples have also been done. The aim of this research was determined of radiological impacts in the environment around the Kartini reactor. The water, soil and plant samples were counted of gross beta, Sr-90 activity by beta counter and for K-40, Cs-137 activity by low background gamma spectrometer. For this evaluation can be concluded there are no indication of the radioactivity release from the Kartini reactor operation. Gross beta radioactivity in the water, soil and grass sample are between 0.06-0.61 Bq/l, 0.24-0.79 Bq/g and 3.47-5.70 Bq/g ash. Radioactivity of K-40 in the water, soil and grass sample are between 0.09-0.56 Bq/l, 0.12-0.59 Bq/g and 0.29-0.93 Bq/g ash. The radioactivity of Cs-137 in the water samples are between under limit detectable level to 88.62 mBq/l and Sr-90 are between under limit detectable level to 24.22 mBq/l. (author)

  9. Study on the effect of moderator density reactivity for Kartini reactor

    International Nuclear Information System (INIS)

    Budi Rohman; Widarto

    2009-01-01

    One of important characteristics of water-cooled reactors is the change of reactivity due to change in the density of coolant or moderator. This parameter generally has negative value and it has significant role in preventing the excursion of power during operation. Many thermal-hydraulic codes for nuclear reactors require this parameter as the input to account for reactivity feedback due to increase in moderator voids and the subsequent decrease in moderator density during operation. Kartini reactor is cooled and moderated by water, therefore, it is essential to study the effect of the change in moderator density as well as to determine the value of void or moderator density reactivity coefficient in order to characterize its behavior resulting from the presence of vapor or change of moderator density during operation. Analysis by MCNP code shows that the reactivity of core is decreasing with the decrease in moderator density. The analysis estimates the void or moderator density reactivity coefficient for Kartini Reactor to be -2.17×10-4 Δρ/ % void . (author)

  10. Ageing Management Programme: An Experience of In-Service Inspection of the Kartini Heat Exchanger

    Energy Technology Data Exchange (ETDEWEB)

    Nitiswati, S., E-mail: nitis@batan.go.id [Centre for Reactor Technology and Nuclear Safety, National Nuclear Energy Agency (BATAN), Jakarta (Indonesia); Syarip,; Tjiptono, T.; Wantana, [Centre for Accelerator and Material Process Technology, National Nuclear Energy Agency (BATAN), Yogyakarta (Indonesia)

    2014-08-15

    This paper discusses an experience on ISI of the Kartini reactor heat exchanger, as part of the implementation of an ageing management programme. Kartini reactor is located in Yogyakarta, Indonesia. The heat exchanger was constructed for 250 kW capacity. The type of heat exchanger is shell with tube recirculation. Tube material is stainless steel 304, and the shell and baffle plate materials are carbon steel. The heat exchanger has 72 tubes, its outer and inner diameters respectively are 19 and 16 mm, and tube thickness is 1.5 mm. The aim of ISI was to obtain and evaluate the heat exchanger’s condition including the detection of any possible local tube thinning, pitting corrosion or gradual thinning, and determine whether any degradation or deterioration of the heat exchanger could have a significant impact to safety. The heat exchanger was inspected utilizing eddy current equipment in 2003 and 2006. Inspection results in 2003 determined that 12 heat exchanger tubes have a thinning degradation level ranging from 10% up to 60% of the outer diameter due to pitting corrosion. Deterioration of baffle plates has been linked to general corrosion attack. Inspection results in 2006 showed a consistent thinning degradation level with the previous inspection in 2003. So far heat exchanger performance is still satisfactory, as defined by the transfer of primary heat for a 2°C difference between inlet and outlet as required. (author)

  11. Safety Management and Safety Culture Self Assessment of Kartini Research Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Syarip, S., E-mail: syarip@batan.go.id [Centre for Accelerator and Material Process Technology, National Nuclear Energy Agency (BATAN), Yogyakarta (Indonesia)

    2014-10-15

    The self-assessment of safety culture and safety management status of Kartini research reactor is a step to foster safety culture and management by identifying good practices and areas for improvement, and also to improve reactor safety in a whole. The method used in this assessment is based on questionnaires provided by the Forum for Nuclear Cooperation in Asia (FNCA), then reviewed by experts. Based on the assessment and evaluation results, it can be concluded that there were several good practices in maintaining the safety status of Kartini reactor such as: reactor operators and radiation protection workers were aware and knowledgeable of the safety standards and policies that apply to their operation, readily accept constructive criticism from their management and from the inspectors of regulatory body that address safety performance. As a proof, for the last four years the number of inspection/audit findings from Regulatory Body (BAPETEN) tended to decrease while the reactor utilization and its operating hour increased. On the other hands there were also some comments and recommendations for improvement of reactor safety culture, such as that there should be more frequent open dialogues between employees and managers, to grow and attain a mutual support to achieve safety goals. (author)

  12. A CONCEPTUAL DESIGN OF NEUTRON COLLIMATOR IN THE THERMAL COLUMN OF KARTINI RESEARCH REACTOR FOR IN VITRO AND IN VIVO TEST OF BORON NEUTRON CAPTURE THERAPY

    Directory of Open Access Journals (Sweden)

    Nina Fauziah

    2015-03-01

    Full Text Available Studies were carried out to design a collimator which results in epithermal neutron beam for IN VITRO and IN VIVO of Boron Neutron Capture Therapy (BNCT at the Kartini research reactor by means of Monte Carlo N-Particle (MCNP codes. Reactor within 100 kW of thermal power was used as the neutron source. The design criteria were based on recommendation from the International Atomic Energy Agency (IAEA. All materials used were varied in size, according to the value of mean free path for each material. MCNP simulations indicated that by using 5 cm thick of Ni as collimator wall, 60 cm thick of Al as moderator, 15 cm thick of 60Ni as filter, 2 cm thick of Bi as γ-ray shielding, 3 cm thick of 6Li2CO3-polyethylene as beam delimiter, with 1 to 5 cm varied aperture size, epithermal neutron beam with maximum flux of 7.65 x 108 n.cm-2.s-1 could be produced. The beam has minimum fast neutron and γ-ray components of, respectively, 1.76 x 10-13 Gy.cm2.n-1 and 1.32 x 10-13 Gy.cm2.n-1, minimum thermal neutron per epithermal neutron ratio of 0.008, and maximum directionality of 0.73. It did not fully pass the IAEA’s criteria, since the epithermal neutron flux was below the recommended value, 1.0 x 109 n.cm-2.s-1. Nonetheless, it was still usable with epithermal neutron flux exceeding 5.0 x 108 n.cm-2.s-1. When it was assumed that the graphite inside the thermal column was not discharged but only the part which was going to be replaced by the collimator, the performance of the collimator became better within the positive effect from the surrounding graphite that the beam resulted passed all criteria with epithermal neutron flux up to 1.68 x 109 n.cm-2.s-1. Keywords: design, collimator, epithermal neutron beam, BNCT, MCNP, criteria   Telah dilakukan penelitian tentang desain kolimator yang menghasilkan radiasi netron epitermal untuk uji in vitro dan in vivo pada Boron Neutron Capture Therapy (BNCT di Reaktor Riset Kartini dengan menggunakan program Monte

  13. Application Of WIMS Code To Calculation Kartini Reactor Parameters By Pin-Cell And Cluster Method

    International Nuclear Information System (INIS)

    Sumarsono, Bambang; Tjiptono, T.W.

    1996-01-01

    Analysis UZrH fuel element parameters calculation in Kartini Reactor by WIMS Code has been done. The analysis is done by pin cell and cluster method. The pin cell method is done as a function percent burn-up and by 8 group 3 region analysis and cluster method by 8 group 12 region analysis. From analysis and calculation resulted K ∼ = 1.3687 by pin cell method and K ∼ = 1.3162 by cluster method and so deviation is 3.83%. By pin cell analysis as a function percent burn-up at the percent burn-up greater than 59.50%, the multiplication factor is less than one (k ∼ < 1) it is mean that the fuel element reactivity is negative

  14. Finite Element Method in the Three Dimensions Deformation Computation ofKartini Reactor Stack

    International Nuclear Information System (INIS)

    Supriyono; Syarip; Wibisono, I

    2000-01-01

    The calculation of the Kartini reactor stack i.e. one of the nuclearinstallations in P3TM-BATAN Yogyakarta by using SAP 90 software have beendone. The calculation is done as a safety review of building towards theearthquake style in Yogyakarta. The 3-dimension deformation calculation isperformed by the numeric method i.e. finite element method with the form ofelements is the shell. The result obtained showed that the construction oftower safe to the existing earthquake, where the moment exerted as a resultof earthquake style was different under the moment having been kept by thebuilding structure. By knowing the deformation on the stack it is expectedcould be used for concluding the strength of the whole reactor building.(author)

  15. Design of Computerized in Core Fuel Management System of Kartini Reactor

    International Nuclear Information System (INIS)

    Edi-Trijono-Budisantoso; Sardjono, Y; Edi-Purwanto; Widi-Setiawan

    2000-01-01

    The program organization for managing Kartini reactor fuel elements has been designed. This program organization work to process on-line operationdata-base and core configuration data-base to produce data-base for in-corefuer management. The in-core fuel management data-base consist of irradiationhistory card, radionuclides inventory and radiation dose for each fuelelement. The computation in this process based on the ORIGEN2, TRIGAP codesand some in-house developed codes that perform matching between output dataof many codes to output data of other code. This program organization worksunder control of a program manager by following the scheduled time table. Thedesign gives a description of the first step development of the in-core fuelmanagement that will be implemented in the internet web server. (author)

  16. ATR Spent Fuel Options Study

    International Nuclear Information System (INIS)

    Connolly, Michael James; Bean, Thomas E.; Brower, Jeffrey O.; Luke, Dale E.; Patterson, M. W.; Robb, Alan K.; Sindelar, Robert; Smith, Rebecca E.; Tonc, Vincent F.; Tripp, Julia L.; Winston, Philip L.

    2017-01-01

    The Advanced Test Reactor (ATR) is a materials and fuels test nuclear reactor that performs irradiation services for the U.S. Department of Energy (DOE) Office of Nuclear Energy (NE), Naval Reactors, the National Nuclear Security Administration (NNSA), and other research programs. ATR achieved initial criticality in 1967 and is expected to operate in support of needed missions until the year 2050 or beyond. It is anticipated that ATR will generate approximately 105 spent nuclear fuel (SNF) elements per year through the year 2050. Idaho National Laboratory (INL) currently stores 2,008 ATR SNF elements in dry storage, 976 in wet storage, and expects to have 1,000 elements in wet storage before January 2017. A capability gap exists at INL for long-term (greater than the year 2050) management, in compliance with the Idaho Settlement Agreement (ISA), of ATR SNF until a monitored retrievable geological repository is open. INL has significant wet and dry storage capabilities that are owned by the DOE Office of Environmental Management (EM) and operated and managed by Fluor Idaho, which include the Idaho Nuclear Technology and Engineering Center's (INTEC's) CPP-666, CPP-749, and CPP-603. In addition, INL has other capabilities owned by DOE-NE and operated and managed by Battelle Energy Alliance, LLC (BEA), which are located at the Materials and Fuel Complex (MFC). Additional storage capabilities are located on the INL Site at the Naval Reactors Facility (NRF). Current INL SNF management planning, as defined in the Fluor Idaho contract, shows INTEC dry fuel storage, which is currently used for ATR SNF, will be nearly full after transfer of an additional 1,000 ATR SNF from wet storage. DOE-NE tasked BEA with identifying and analyzing options that have the potential to fulfill this capability gap. BEA assembled a team comprised of SNF management experts from Fluor Idaho, Savannah River Site (SRS), INL/BEA, and the MITRE Corp with an objective of developing and analyzing

  17. ATR Spent Fuel Options Study

    Energy Technology Data Exchange (ETDEWEB)

    Connolly, Michael James [Idaho National Lab. (INL), Idaho Falls, ID (United States); Bean, Thomas E. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Brower, Jeffrey O. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Luke, Dale E. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Patterson, M. W. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Robb, Alan K. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Sindelar, Robert [Idaho National Lab. (INL), Idaho Falls, ID (United States); Smith, Rebecca E. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Tonc, Vincent F. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Tripp, Julia L. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Winston, Philip L. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2017-01-01

    The Advanced Test Reactor (ATR) is a materials and fuels test nuclear reactor that performs irradiation services for the U.S. Department of Energy (DOE) Office of Nuclear Energy (NE), Naval Reactors, the National Nuclear Security Administration (NNSA), and other research programs. ATR achieved initial criticality in 1967 and is expected to operate in support of needed missions until the year 2050 or beyond. It is anticipated that ATR will generate approximately 105 spent nuclear fuel (SNF) elements per year through the year 2050. Idaho National Laboratory (INL) currently stores 2,008 ATR SNF elements in dry storage, 976 in wet storage, and expects to have 1,000 elements in wet storage before January 2017. A capability gap exists at INL for long-term (greater than the year 2050) management, in compliance with the Idaho Settlement Agreement (ISA), of ATR SNF until a monitored retrievable geological repository is open. INL has significant wet and dry storage capabilities that are owned by the DOE Office of Environmental Management (EM) and operated and managed by Fluor Idaho, which include the Idaho Nuclear Technology and Engineering Center’s (INTEC’s) CPP-666, CPP-749, and CPP-603. In addition, INL has other capabilities owned by DOE-NE and operated and managed by Battelle Energy Alliance, LLC (BEA), which are located at the Materials and Fuel Complex (MFC). Additional storage capabilities are located on the INL Site at the Naval Reactors Facility (NRF). Current INL SNF management planning, as defined in the Fluor Idaho contract, shows INTEC dry fuel storage, which is currently used for ATR SNF, will be nearly full after transfer of an additional 1,000 ATR SNF from wet storage. DOE-NE tasked BEA with identifying and analyzing options that have the potential to fulfill this capability gap. BEA assembled a team comprised of SNF management experts from Fluor Idaho, Savannah River Site (SRS), INL/BEA, and the MITRE Corp with an objective of developing and analyzing

  18. Steady state and LOCA analysis of Kartini reactor using RELAP5/SCDAP code: The role of passive system

    Science.gov (United States)

    Antariksawan, Anhar R.; Wahyono, Puradwi I.; Taxwim

    2018-02-01

    Safety is the priority for nuclear installations, including research reactors. On the other hand, many studies have been done to validate the applicability of nuclear power plant based best estimate computer codes to the research reactor. This study aims to assess the applicability of the RELAP5/SCDAP code to Kartini research reactor. The model development, steady state and transient due to LOCA calculations have been conducted by using RELAP5/SCDAP. The calculation results are compared with available measurements data from Kartini research reactor. The results show that the RELAP5/SCDAP model steady state calculation agrees quite well with the available measurement data. While, in the case of LOCA transient simulations, the model could result in reasonable physical phenomena during the transient showing the characteristics and performances of the reactor against the LOCA transient. The role of siphon breaker hole and natural circulation in the reactor tank as passive system was important to keep reactor in safe condition. It concludes that the RELAP/SCDAP could be use as one of the tool to analyse the thermal-hydraulic safety of Kartini reactor. However, further assessment to improve the model is still needed.

  19. ATR Technical Specification Upgrade Program

    International Nuclear Information System (INIS)

    McCracken, R.T.; Durney, J.L.; Freund, G.A.

    1990-01-01

    The Advanced Test Reactor (ATR) is a 250 MW, uranium-aluminum fueled test reactor which began full power operation in 1969. The initial operation was controlled by an Operating Limits document based on the original Safety Analysis Report. Additional safety bases were later developed to support Technical Specifications which were approved and implemented in 1977. The Technical Specifications which were initially developed with content and format specified in ANSI/ANS--15.1, ''The Development of Technical Specifications for Research Reactors.'' The safety basis documentation and the Technical Specifications have been updated as required to maintain them current with the ATR facility configuration. All revisions have been made with a content, format and style consistent with the original. A major, two-phase program to upgrade the content, format and style is in progress. This paper describes the first phase of this program

  20. Mox fuel utilization in ATR

    OpenAIRE

    下村 和生; 川太 徳夫

    1987-01-01

    ATR, a heavy-water moderated boiling-light-water cooled reactor developed in Japan, is a unique reactor with out-standing flexibility regarding nuclear fuel utilization, because it has superior properties concerning the utilization of plutonium, recovered uranium and depleted uranium. The development of this type of reactor is expected to contribute both to the stable supply of energy and to the establishment of plutonium utilization in Japan. Much effort has been and will be made on the deve...

  1. Kartini reactor tank inspection using NDT method for safety improvement of the reactor operation

    International Nuclear Information System (INIS)

    Syarip; Sutondo, Tegas; Saleh, Chaerul; Nitiswati; Puradwi; Andryansah; Mudiharjo

    2002-01-01

    The inspection of Kartini reactor tank liner (TRK) by using Non Destructive Testing (NDT) methods to improve the reactor operation safety, have been done. The type of NDT used were: visual examination using an underwater camera and magnifier, replication survey using dental putty, hardness test using an Equotip D indentor, thickness test using ultrasonic probe, and dye penetrant test. The visual examination showed that the surface of TRK was in good condition. The hardness readings were considered to be consistent with the original condition of the tank and the slight hardness increase at the reactor core area consistent with the neutron fluence experienced -10 1 4 n/cm 2 . Results of ultrasonic thickness survey showed that in average the TRK thickness is between 5,0 mm - 6,5 mm, a low 2,1 mm thickness exists at the top of the TRK in the belt area (double layer aluminum plat, therefore do not influencing the safety ). The replica and dye penetrant test at the low thickness area and several suspected areas showed that it could be some defect from original manufacture. Therefore, it can be concluded that the TRK is still feasible for continued operation safely

  2. Application of fuzzy set theory for safety culture and safety management assessment of Kartini research reactor

    International Nuclear Information System (INIS)

    Syarip; Hauptmanns, U.

    2000-01-01

    The safety culture status of nuclear power plant is usually assessed through interview and/or discussions with personnel and management in plant, and an assessment of the pertinent documentation. The approach for safety culture assessment described in IAEA Safety Series, make uses of a questionnaire composed of questions which require 'Yes' or 'No' as an answer. Hence, it is basically a check-list approach which is quite common for safety assessments in industry. Such a procedure ignores the fact that the expert answering the question usually has knowledge which goes far beyond a mere binary answer. Additionally, many situations cannot readily be described in such restricted terms. Therefore, it was developed a checklist consisting of questions which are formulated such that they require more than a simple 'yes' or 'no' as an answer. This allows one to exploit the expert knowledge of the analyst appropriately by asking him to qualify the degree of compliance of each of the topics examined. The method presented has proved useful in assessing the safety culture and quality of safety management of the research reactor. The safety culture status and the quality of safety management of Kartini research reactor is rated as 'average'. The method is also flexible and allows one to add questions to existing areas or to introduce new areas covering related topics

  3. 77 FR 67557 - Special Conditions: ATR-GIE Avions de Transport Regional, Models ATR42-500 and ATR72-212A...

    Science.gov (United States)

    2012-11-13

    ..., Models ATR42-500 and ATR72-212A Airplanes; Aircraft Electronic System Security Protection From... exploited by unauthorized access to airplane systems, data buses, and servers. Therefore, these special... ATR42-500 and ATR72-212A airplanes. 1. Airplane Electronic System Security Protection from Unauthorized...

  4. Perumusan Model Moneter Berdasarkan Perilaku Gas Ideal

    Directory of Open Access Journals (Sweden)

    Rachmad Resmiyanto

    2014-04-01

    Full Text Available Telah disusun sebuah model moneter yang berdasarkan perilaku gas ideal. Model disusun dengan menggunakan metode kias/analogi. Model moneter gas ideal mengiaskan jumlah uang beredar dengan volume gas, daya beli dengan tekanan gas dan produksi barang dengan suhu gas. Model ini memiliki formulasi yang berbeda dengan Teori Kuantitas Uang (Quantity Theory of Money yang dicetuskan oleh Irving Fisher, model moneter Marshal-Pigou dari Cambridge serta model moneter ala Keynes. Selama ini 3 model tersebut dianggap sebagai model yang mapan dalam teori moneter pada buku-buku teks ekonomi. Model moneter gas ideal dapat menjadi cara pandang baru terhadap sistem moneter.

  5. The Design of Sample Driver System for Gamma Irradiator Facility at Thermal Column of Kartini Reactor

    International Nuclear Information System (INIS)

    Suyamto; Tasih Mulyono; Setyo Atmojo

    2007-01-01

    The design and construction of sample driver system for gamma irradiator facility at thermal column of Kartini reactor post operation has been carried out. The design and construction is based on the space of thermal column and the sample speed rotation which has to as low as possible in order the irradiation process can be more homogeneity. The electrical and mechanical calculation was done after fixation the electrical motor and transmission system which will be applied. By the assumption that the maximum sample weight is 50 kg, the electric motor specification is decided due to its rating i.e. single phase induction motor, run capacitor type, 0.5 HP; 220 V; 3.61 A, CCW and CW, rotation speed 1430 rpm. To achieve the low load rotation speed, motor speed was reduced twice using the conical reduction gear with the reduction ratio 3.9 and thread reduction gear with the reduction ratio 60. From the calculation it is found that power of motor is 118.06 watt, speed rotation of load sample is 6.11 rpm due to the no load rotation of motor 1430 rpm. From the test by varying weight of load up to 75 kg it is known that the device can be operated in a good condition, both in the two direction with the average speed of motor 1486 rpm and load 6.3 rpm respectively. So that the slip is 0.268 % and 0.314 % for no load and full load condition. The difference input current to the motor during no load and full load condition is relative small i.e. 0.14 A. The safety factor of motor is 316 % which is correspond to the weight of load 158 kg. (author)

  6. ATR Commissioning Software Task Force Report

    Energy Technology Data Exchange (ETDEWEB)

    D' Ottavio, Ted [Brookhaven National Lab. (BNL), Upton, NY (United States); Kewisch, Jorg [Brookhaven National Lab. (BNL), Upton, NY (United States); Saltmarsh, Chris [Brookhaven National Lab. (BNL), Upton, NY (United States); Sathe, Smita [Brookhaven National Lab. (BNL), Upton, NY (United States); Satogata, Todd [Brookhaven National Lab. (BNL), Upton, NY (United States); Shea, Don [Brookhaven National Lab. (BNL), Upton, NY (United States); Tepikian, Steve [Brookhaven National Lab. (BNL), Upton, NY (United States); Trahern, Garry [Brookhaven National Lab. (BNL), Upton, NY (United States)

    1994-12-16

    The Beam Injection Tests Software Task Force was charged with studying the software needed for the ATR tests, seen as a stepping stone or template for the larger scope of the full RHIC control system. This report outlines our avenues of exploration so far, presents the current analysis and implementation work in progress, and gives recommendations for the future on the ATR and longer time scales.

  7. The effect of leadership, organizational culture, and competency on teachers' performance in Ibu Kartini vocational high school Semarang

    Science.gov (United States)

    Toha, Mohamad; Katoningsih, Sri

    2018-03-01

    The low performance of teachers in organization is influenced many factors. Organizational culture could be the key of organization success; hence many researches were done to identify the value and the attitude norm that gave big contribution for organization success. Competency is a part of employee they perform during work as kind of behavior. Competency depends on the aspects process of teachers' performance. The purpose of this research is to know the effect of leadership, organizational culture and competency on teachers' performance. The objects of this research are leadership, organizational culture, competency and teachers' performance in Ibu Kartini vocational high school. This research is quantitative. To collect the data, questionnaire was used. Then, the data were analyzed by using Path analysis in SPPS 16. The result of this research showed that leadership, organizational culture, competency and performance run well and had significant effect on teachers' performance.

  8. Kepuasan Kerja dan Dampaknya terhadap Unjuk Kerja Guru di Gugus Pantai Kartini UPT Dinas Pendidikan Kecamatan Mojolaban (Tinjauan Aspek Keteladanan Pimpinan dan Ketrampilan Pemanfaatan Media

    Directory of Open Access Journals (Sweden)

    Emi Sugiati

    2015-07-01

    Full Text Available This research aims to describe : 1 the contribution of leader exemplary and learning media model through indirect satisfied concerning the characteristic of the teacher performance, 2 The contribution of leader exemplary and the satisfied learning media model, 3 the satisfied of the teacher performance. The result is expected to gave information to the headmaster, so its can become reconciliation to increase the school work and for the teacher they can get a feet-back about the increase of the work. This quantitative research has used inquiry method that proved valid and reliable and documentations method a means of principal data collecting, and to test direct and indirect contribution between keteladanan leadership, study media, satisfaction and performance teacher at coast cluster Kartini UPT district Mojolaban education official use stripe analysis method (path Analysis. population in these watchfulness teachers coast cluster Pantai Kartini UPT district education official Mojolaban as much as 141 person, with sample as much as 106 person. The result show that : 1 The leader exemplary and learning media give direct contribution for the teacher performance, 2 The leader exemplary and learning media give direct significant to the satisfied, 3 The satisfied give direct contribution and significant for the performance of the teacher in coast cluster Pantai Kartini Education Official UPTD Mojolaban sub district.

  9. Advance of core design method for ATR

    International Nuclear Information System (INIS)

    Maeda, Seiichirou; Ihara, Toshiteru; Iijima, Takashi; Seino, Hideaki; Kobayashi, Tetsurou; Takeuchi, Michio; Sugawara, Satoru; Matsumoto, Mitsuo.

    1995-01-01

    Core characteristics of ATR demonstration plant has been revised such as increasing the fuel burnup and the channel power, which is achieved by changing the number of fuel rod per fuel assembly from 28 to 36. The research and development concerning the core design method for ATR have been continued. The calculational errors of core analysis code have been evaluated using the operational data of FUGEN and the full scale simulated test results in DCA (Deuterium Critical Assembly) and HTL (Heat Transfer Loop) at O-arai engineering center. It is confirmed that the calculational error of power distribution is smaller than the design value of ATR demonstration plant. Critical heat flux correlation curve for 36 fuel rod cluster has been developed and the probability evaluation method based on its curve, which is more rational to evaluate the fuel dryout, has been adopted. (author)

  10. Focusing and matching properties of the ATR transfer line

    International Nuclear Information System (INIS)

    Tsoupas, N.; Fischer, W.; Kewisch, J.; MacKay, W.W.; Peggs, S.; Pilat, F.; Tepikian, S.; Wei, J.

    1997-01-01

    The AGS to RHIC (AtR) beam transfer line has been constructed and will be used to transfer beam bunches from the AGS machine into the RHIC machine which is presently under construction at BNL. The original design of the AtR line has been modified. This article will present the optics of the various sections of the existing AtR beam line, as well as the matching capabilities of the AtR line to the RHIC machine

  11. On techniques of ATR lattice computation

    International Nuclear Information System (INIS)

    1997-08-01

    Lattice computation is to compute the average nuclear constants of unit fuel lattice which are required for computing core nuclear characteristics such as core power distribution and reactivity characteristics. The main nuclear constants are infinite multiplying rate, neutron movement area, cross section for diffusion computation, local power distribution and isotope composition. As for the lattice computation code, WIMS-ATR is used, which is based on the WIMS-D code developed in U.K., and for the purpose of heightening the accuracy of analysis, which was improved by adding heavy water scattering cross section considering the temperature dependence by Honeck model. For the computation of the neutron absorption by control rods, LOIEL BLUE code is used. The extrapolation distance of neutron flux on control rod surfaces is computed by using THERMOS and DTF codes, and the lattice constants of adjoining lattices are computed by using the WIMS-ATR code. As for the WIMS-ATR code, the computation flow and nuclear data library, and as for the LOIEL BLUE code, the computation flow are explained. The local power distribution in fuel assemblies determined by the WIMS-ATR code was verified with the measured data, and the results are reported. (K.I.)

  12. ATR prohibits replication catastrophe by preventing global exhaustion of RPA.

    Science.gov (United States)

    Toledo, Luis Ignacio; Altmeyer, Matthias; Rask, Maj-Britt; Lukas, Claudia; Larsen, Dorthe Helena; Povlsen, Lou Klitgaard; Bekker-Jensen, Simon; Mailand, Niels; Bartek, Jiri; Lukas, Jiri

    2013-11-21

    ATR, activated by replication stress, protects replication forks locally and suppresses origin firing globally. Here, we show that these functions of ATR are mechanistically coupled. Although initially stable, stalled forks in ATR-deficient cells undergo nucleus-wide breakage after unscheduled origin firing generates an excess of single-stranded DNA that exhausts the nuclear pool of RPA. Partial reduction of RPA accelerated fork breakage, and forced elevation of RPA was sufficient to delay such "replication catastrophe" even in the absence of ATR activity. Conversely, unscheduled origin firing induced breakage of stalled forks even in cells with active ATR. Thus, ATR-mediated suppression of dormant origins shields active forks against irreversible breakage via preventing exhaustion of nuclear RPA. This study elucidates how replicating genomes avoid destabilizing DNA damage. Because cancer cells commonly feature intrinsically high replication stress, this study also provides a molecular rationale for their hypersensitivity to ATR inhibitors. Copyright © 2013 Elsevier Inc. All rights reserved.

  13. Determination and analysis of neutron flux distribution on radial Piercing beam port for utilization of Kartini research reactor

    International Nuclear Information System (INIS)

    Widarto

    2002-01-01

    Determination and analysis of neutron flux measurements on radial piercing beam port have been done as completion experimental data document and progressing on utilization of the Kartini research reactor purposes. The analysis and determination of the neutron flux have been carried out by using Au foils detector neutron activation analysis method which put on the radius of cross section (19 cm) and a long of radial piercing beam port (310 cm) Based on the calculation, distribution of the thermal neutron flux is around (8.3 ± 0.9) x 10 5 ncm -2 s -1 to (6.8 ± 0.5) x 10 7 ncm -2 s -1 and fast neutron is (5.0 ± 0.2) x 10 5 ncm -2 s -1 to (1.43 ± 0.6) x 10 7 ncm -2 s -1 . Analyzing by means of curve fitting method could be concluded that the neutron flux distribution on radial piercing beam port has profiled as a polynomial curve. (author)

  14. Monitoring of gross beta radioactivities on water sample environment in the surrounding of kartini reactor at 2011

    International Nuclear Information System (INIS)

    Siswanti; Munandar, A. Aris

    2013-01-01

    Measurement of gross beta radioactivities on water environment were done in the PTAPB BATAN has a goal for routine monitoring, with the result that fill RPL has been made and the result equivalented with quality standard were decided by BAPETEN. The water sample taken as much as 2 liter at 18 area were definited on radius 100 m to 5000 m in the surrounding of kartini reactor, vaporin on electric stove till the volume been ± 10 ml, and than pick out to the aluminium planset and drying on hot plate. Sample in the plancet were counted with a Low Background Counter (LBC) for 30 minutes and accounted of gross beta radioactivity water system. The result of gross beta radioactivity water environment at 2011 has a lowest 009, ± 0,06 Bq/I on Tambak Bayan area at june and in the Janti area highest 0,39 ± 0,08 Bq/ at December. The result still under of quality standard were decided by SK BAPETEN. No. 02/Ka- BAPETEN/V-99 is 0,4 Bq/I. (author)

  15. Hubungan Obesitas Remaja dengan Hipertrofi Ventrikel Kanan berdasarkan Pemeriksaan Elektrokardiografi

    OpenAIRE

    Shinta Larasaty; Julistio Djais; Sri Endah Rahayuningsih

    2016-01-01

    Latar belakang.Perubahan distribusi lemak viseral dan subkutan menyebabkan remaja berisiko mengalami obesitas. Obesitas sering dikaitkan dengan hipoksia kronik akibat obesity hypoventilation syndrome, yang dapat menyebabkan hipertensi pulmonal dan hipertrofi ventrikel kanan. Tujuan. Mengetahui hubungan obesitas remaja dengan hipertrofi ventrikel kanan berdasarkan pemeriksaan EKG. Metode. Studi komparatif dengan rancangan potong lintang, dilakukan Juli–September 2010, pada re...

  16. Keragaman Genetik Plasma Nutfah Rambutan Di Indonesia Berdasarkan Karakter Morfologi

    OpenAIRE

    Kuswandi, Kuswandi; Sobir, Sobir; Suwarno, Willy Bayuardi

    2014-01-01

    Rambutan merupakan tanaman menyerbuk silang sehingga secara alami memiliki keragaman tinggi. Penelitian bertujuan mempelajari kemiripan genetik dan pengelompokan aksesi plasma nutfah rambutan (Nephelium lappaceum) dan kapulasan (Nephelium ramboutan-ake) di Indonesia berdasarkan karakteristik morfologi. Penelitian dilakukan di (1) Kebun Percobaan (KP) Aripan Balai Penelitian Tanaman Buah Tropika, (2) KP Subang Balai Penelitian Tanaman Buah Tropika, (3) KP Cipaku Balai Pengkajian Teknologi Pert...

  17. Function of the ATR N-terminal domain revealed by an ATM/ATR chimera

    International Nuclear Information System (INIS)

    Chen Xinping; Zhao Runxiang; Glick, Gloria G.; Cortez, David

    2007-01-01

    The ATM and ATR kinases function at the apex of checkpoint signaling pathways. These kinases share significant sequence similarity, phosphorylate many of the same substrates, and have overlapping roles in initiating cell cycle checkpoints. However, they sense DNA damage through distinct mechanisms. ATR primarily senses single stranded DNA (ssDNA) through its interaction with ATRIP, and ATM senses double strand breaks through its interaction with Nbs1. We determined that the N-terminus of ATR contains a domain that binds ATRIP. Attaching this domain to ATM allowed the fusion protein (ATM*) to bind ATRIP and associate with RPA-coated ssDNA. ATM* also gained the ability to localize efficiently to stalled replication forks as well as double strand breaks. Despite having normal kinase activity when tested in vitro and being phosphorylated on S1981 in vivo, ATM* is defective in checkpoint signaling and does not complement cellular deficiencies in either ATM or ATR. These data indicate that the N-terminus of ATR is sufficient to bind ATRIP and to promote localization to sites of replication stress

  18. MIR-ATR sensor for process monitoring

    International Nuclear Information System (INIS)

    Geörg, Daniel; Schalk, Robert; Beuermann, Thomas; Methner, Frank-Jürgen

    2015-01-01

    A mid-infrared attenuated total reflectance (MIR-ATR) sensor has been developed for chemical reaction monitoring. The optical setup of the compact and low-priced sensor consists of an IR emitter as light source, a zinc selenide (ZnSe) ATR prism as boundary to the process, and four thermopile detectors, each equipped with an optical bandpass filter. The practical applicability was tested during esterification of ethanol and formic acid to ethyl formate and water as a model reaction with subsequent distillation. For reference analysis, a Fourier transform mid-infrared (FT-MIR) spectrometer with diamond ATR module was applied. On-line measurements using the MIR-ATR sensor and the FT-MIR spectrometer were performed in a bypass loop. The sensor was calibrated by multiple linear regression in order to link the measured absorbance in the four optical channels to the analyte concentrations. The analytical potential of the MIR-ATR sensor was demonstrated by simultaneous real-time monitoring of all four chemical substances involved in the esterification and distillation process. The temporal courses of the sensor signals are in accordance with the concentration values achieved by the commercial FT-MIR spectrometer. The standard error of prediction for ethanol, formic acid, ethyl formate, and water were 0.38 mol L   −  1 , 0.48 mol L   −  1 , 0.38 mol L   −  1 , and 1.12 mol L   −  1 , respectively. A procedure based on MIR spectra is presented to simulate the response characteristics of the sensor if the transmission ranges of the filters are varied. Using this tool analyte specific bandpass filters for a particular chemical reaction can be identified. By exchanging the optical filters, the sensor can be adapted to a wide range of processes in the chemical, pharmaceutical, and beverage industries. (paper)

  19. Cytoplasmic ATR Activation Promotes Vaccinia Virus Genome Replication

    Directory of Open Access Journals (Sweden)

    Antonio Postigo

    2017-05-01

    Full Text Available In contrast to most DNA viruses, poxviruses replicate their genomes in the cytoplasm without host involvement. We find that vaccinia virus induces cytoplasmic activation of ATR early during infection, before genome uncoating, which is unexpected because ATR plays a fundamental nuclear role in maintaining host genome integrity. ATR, RPA, INTS7, and Chk1 are recruited to cytoplasmic DNA viral factories, suggesting canonical ATR pathway activation. Consistent with this, pharmacological and RNAi-mediated inhibition of canonical ATR signaling suppresses genome replication. RPA and the sliding clamp PCNA interact with the viral polymerase E9 and are required for DNA replication. Moreover, the ATR activator TOPBP1 promotes genome replication and associates with the viral replisome component H5. Our study suggests that, in contrast to long-held beliefs, vaccinia recruits conserved components of the eukaryote DNA replication and repair machinery to amplify its genome in the host cytoplasm.

  20. Konsep Cantik pada Iklan Cetak Majalah Kartini Tahun 2014 dan Koran Kompas Tahun 1979: Suatu Analisis Semiotik

    Directory of Open Access Journals (Sweden)

    Dian Natashia

    2015-11-01

    Full Text Available Advertisements, which can be found in daily life, do not just function as the source of the product’s information, but can also create a new connotation about social reality. This research is intended to reveal the form of the connotation about present beauty of Indonesian women that are trying to be conveyed by the advertisers through present advertisements in Kartini magazines.  This research is also revealing elements that are used by the advertisers to attract readers’ attention. To see what are the new connotation that are formed in the present advertisements, they will be compared with advertisements in the older generation. This research is using qualitative descriptive method and the data is coming from the wordings that are printed in those advertisements. The data will be described and analyzed with Discourse of Advertising Theory by Guy Cook and Anchoring Connotation Theory by Roland Barthes, and then the result will be conclude. Through Guy’s theory, this research will reveal all the elements that are used by the producers to build the beauty concept. Through Barthes’ theory, this research will show how the advertisers create new connotation. The result of analysis shows that present beauty concept about ideal body is a beautiful body that are radiated from the outer side of the women’s body and good looking. Beautiful face is a face that looks as young as twenty years old girls. Moreover, the result also shows that producers use current social representative where consumers these days like new, instant products that give maximum result. The result also shows consumers’ doubt over unknown products and women’s fear on fat body and aging.

  1. Design of collimator in the radial piercing beam port of Kartini reactor for boron neutron capture therapy

    International Nuclear Information System (INIS)

    M Ilma Muslih A; Andang Widiharto; Yohannes Sardjono

    2014-01-01

    Studies were carried out to design a collimator which results in epithermal neutron beam for in vivo experiment of Boron Neutron Capture Therapy (BNCT) at the Kartini Research Reactor by means of Monte Carlo N-Particle (MCNP) codes. Reactor within 100 kW of thermal power was used as the neutron source. All materials used were varied in size, according to the value of mean free path for each material. MCNP simulations indicated that by using 5 cm thick of Ni (95%) as collimator wall, 15 cm thick of Al as moderator, 1 cm thick of Pb as γ-ray shielding, 1.5 cm thick of Boral as additional material, with 2 cm aperture diameter, epithermal neutron beam with maximum flux of 5.03 x 10 8 n.cm -2 .s -1 could be produced. The beam has minimum fast neutron and γ-ray components of, respectively, 2.17 x 10 -13 Gy.cm 2 .n -1 and 1.16 x 10 -13 Gy.cm 2 .n -l , minimum thermal neutron per epithermal neutron ratio of 0.12, and maximum directionality of 0.835 . It did not fully pass the IAEA's criteria, since the epithermal neutron flux was below the recommended value, 1.0 x 10 9 n.cm -2 .s -l . Nonetheless, it was still usable with epithermal neutron flux exceeding 5.0 x 10 8 n.cm -2 .s -1 and fast neutron flux close to 2 x 10 -13 Gy.cm 2 .n -1 it is still feasible for BNCT in vivo experiment. (author)

  2. Model Penilaian Kapabilitas Proses Optimasi Resiko Ti Berdasarkan Cobit 5

    OpenAIRE

    Putri, Rahmi Eka

    2015-01-01

    Teknologi informasi (TI) saat ini telah menjadi kebutuhan yang mendasar bagi setiap organisasi dan tidak jarang sebuah organisasi memiliki ketergantungan yang tinggi terhadap TI. Resiko penggunaan TI di organisasi harus dikelola dengan baik, agar resiko penggunaan TI dapat diminimalisasi dan TI dapat membantu dalam pencapaian tujuan organisasi. Optimasi resiko penggunaan TI di organisasi merupakan salah satu domain dalam tata kelola TI berdasarkan COBIT 5. Proses ini bertujuan untuk memastika...

  3. MODEL KESESUAIAN APLIKASI UNTUK GOVERNMENT BERDASARKAN BUDAYA ORGANISASI

    Directory of Open Access Journals (Sweden)

    Sri Handayaningsih

    2013-04-01

    Full Text Available Pengembangan E-Government pada sebuah Pemda ditopang oleh empat pilar yaitu kebijakan, kerangka peraturan, manajemen perubahan dan kebutuhan masyarakat. Proses bisnis pada dinas di pemda dan kebutuhan masyarakat terhadap dinas, memunculkan aplikasi yang dapat membantu proses bisnis dalam memberikan layanan pada masyarakat dan manajemen dalam internal dinas. Dalam banyak kasus pengembangan aplikasi pada Pemda tidak berdasarkan pada hal-hal seperti  proses bisnis yang dijalankan di dinas, budaya organisasi dan kebutuhan masyarakat sebagai konsumen namun hanya berdasarkan pada kebutuhan personal dan pemberian dari pusat. Hal ini mengakibatkan aplikasi yang dibangun tidak dapat bertahan lama, tidak dapat membantu seluruh kegiatan bisnis utama dinas dan dijalankan tidak sesuai dengan budaya organisasi yang sedang berjalan saat ini di dinas tersebut. Aplikasi yang harus dibangun dan dijalankan adalah 70% untuk kegiatan pelayanan pada masyarakat dan 30% manajemen organisasi. Pembuatan model kesesuai aplikasi pada sebuah dinas berdasarkan pada budaya yang sedang berjalan di dinas. Budaya organisasi yang berjalan saat ini di pemerintah provinsi DIY adalah budaya clan. Dasar dari kesesuaian aplikasi yang berjalan di sebuah dinas adalah rencana strategis jangka menengah (tahun 2009 s.d 2014. Model akan diukur dengan key performance indicator menggunakan acuan pada COBIT 4.1. Harapannya model dapat digunakan pada dinas di Provinsi DIY

  4. Kepemimpinan di Perpustakaan Berdasarkan Emotional Intellegience dalam Menghadapi Globalisasi Informasi

    Directory of Open Access Journals (Sweden)

    Miftahunnisa' Igiriza

    2017-06-01

    Full Text Available Pemimpin dalam sebuah kepemimpinan menjadi salah satu faktor maupun penghambat dari perkembangan organisasi dalam menghadapi fenomena perubahan yang kerap terjadi. Kemampuan kecerdasan emosional pemimpin memiliki peran yang penting bagi organisasi dan orang-orang yang bekerja pada organisasi tersebut. Tujuan penulisan ini yaitu untuk mengetahui bahwa kepemimpinan berdasarkan emotional intelligence dibutuhkan dalam gaya memimpin suatu organisasi seperti di perpustakaan yang menjadi pusat informasi. Artikel ini disusun berdasarkan studi literatur. Dapat diketahui, terjadinya globalisasi informasi, pola kepemimpinan yang baik di perpustakaan sangat dibutuhkan. Pola kepemimpinan berdasarkan emotional intelligence yang di dalamnya terdiri dari visioner, pembimbing, afiliatif, demokratis, penentu kecepatan, dan memerintah bisa menjadi pilihan pola kepemimpinan yang diterapkan di perpustakaan.  ABSTRACT Leader in a leadership became one of the factors as well as inhibiting the development of the organization in the face of the phenomenon of changes that often occur. Emotional intelligence abilities leaders have an important role for the organization and the people who work in these organizations. The purpose of this paper is to know that leadership is based on emotional intelligence needed to lead an organization like the style in which the library is a resource center. This article draws on literature studies. It can be seen, the globalization of information, a pattern of good leadership in the library is needed. The pattern of leadership based on the emotional intelligence of which consists of a visionary, coaching, affiliative, democratic, pacesetting, and commanding leadership style choice can be applied in the library.

  5. ATR kinase regulates its attenuation via PPM1D phosphatase ...

    Indian Academy of Sciences (India)

    In eukaryotes, in response to replication stress, DNA damage response kinase, ATR is activated, whose signalling abrogationleads to cell lethality due to aberrant fork remodelling and excessive origin firing. Here we report that inhibition ofATR kinase activity specifically during replication stress recovery results in persistent ...

  6. Safety significance of ATR passive safety response attributes

    International Nuclear Information System (INIS)

    Atkinson, S.A.

    1990-01-01

    The Advanced Test Reactor (ATR) at the Idaho National Engineering Laboratory was designed with some passive safety response attributes which contribute to the safety of the facility. The three passive safety attributes being evaluated in the paper are: 1) In-core and in-vessel natural convection cooling, 2) a passive heat sink capability of the ATR primary coolant system (PCS) for the transfer of decay power from the uninsulated piping to the confinement, and 3) gravity feed of emergency coolant makeup. The safety significance of the ATR passive safety response attributes is that the reactor can passively respond to most transients, given a reactor scram, to provide adequate decay power removal and a significant time for operator action should the normal active heat removal systems and their backup systems both fail. The ATR Interim Level 1 Probabilistic Risk Assessment (PRA) models and results were used to evaluate the significance to ATR fuel damage frequency (or probability) of the above three passive response attributes. The results of the evaluation indicate that the first attribute is a major safety characteristic of the ATR. The second attribute has a noticeable but only minor safety significance. The third attribute has no significant influence on the ATR firewater injection system (emergency coolant system)

  7. Identifiksi Sebaran Litologi berdasarkan Analisis Data Resistivitas di Gunung Wungkal

    Directory of Open Access Journals (Sweden)

    Nurul Dzakiya

    2017-05-01

    Full Text Available Analisis data untuk mengidentifikasi sebaran litologi yang memiliki potensi bahan galian industri di Daerah Gunung Wungkal Yogyakarta dengan menggunakan metode resistivitas dan survei geologi permukaan telah dilakukan. Hasil korelasi kedua data tersebut menunjukkan sebaran litologi merupakan pelapukan intrusi batuan beku yang kemudian menghasilkan material lempung (6,0-10 m, lempung pasiran (11-30 m dan lapukan batuan diorit (30-55 m yang berada di kedalaman berbeda. Ketebalan batuan di permukaan sekitar 225-231 meter berdasarkan penampang peta geologi dengan arah sebaran dari Barat Laut hingga Tenggara. Proses pelapukan dan alterasi di daerah ini intensif (alterasi argilik dengan jenis morfologi perbukitan intrusi terdenudasi dan dataran Alluvial.

  8. ATR Prohibits Replication Catastrophe by Preventing Global Exhaustion of RPA

    DEFF Research Database (Denmark)

    Toledo Lazaro, Luis Ignacio; Altmeyer, Matthias; Rask, Maj-Britt

    2013-01-01

    origin firing generates an excess of single-stranded DNA that exhausts the nuclear pool of RPA. Partial reduction of RPA accelerated fork breakage, and forced elevation of RPA was sufficient to delay such "replication catastrophe" even in the absence of ATR activity. Conversely, unscheduled origin firing...... induced breakage of stalled forks even in cells with active ATR. Thus, ATR-mediated suppression of dormant origins shields active forks against irreversible breakage via preventing exhaustion of nuclear RPA. This study elucidates how replicating genomes avoid destabilizing DNA damage. Because cancer cells...

  9. Evaluation of Radiological Impacts on the Operating Kartini Reactor and Natural Radioactivity of the Site Plan of Nuclear Power Plant Area

    International Nuclear Information System (INIS)

    Yazid, M; Sutresna, G; Sulistyono, A; Ngasifudin

    1996-01-01

    This radiological impacts evaluation covered of radioactivity in water, soil, grass, air samples and ambient gamma radiation that have been carried out in the Kartini reactor area and in the site plan of nuclear power plan are at Ujung Lemah Abang, Jepara, Central Java. The aim of this research was to determine that radiological impacts in the environment around the Kartini reactor compared to natural radioactivity for site plan of nuclear power plan area. The radioactivity in the water, soil and grass samples ware measured by low background beta counting system and were identified by low background gamma spectrometer. The radioactivity in the air samples was measured by beta portable counting system and the ambient gamma radiation was measured by portable high pressurized ionization chamber model RSS-112 Reuther-Stokes. The reactor data measurement was compared to the site plan of nuclear power plant area data for evaluation of radiological impacts on the operating reactor. From the evaluation and comparison can be concluded there are no indication of the radionuclide release from the reactor operation. The average radiactivity in the water, soil grass and air sample from the reactor area were between 0.17 - 0.61 Bq/1; 0,47 - 0,74 Bq/g; 4.43 - 4.60 Bq/g.ash and 49.53 - 70.90 x 10 Bq/cc. The average radioactivity of those sample from the nuclear power plant area were between 0.06-0.90 Bq/I; 0.02-0.86 Bq/g; 1.68-8.07 Bq/g.ash and 65.0-152.3 x 10 Bq/cc. The ambient gamma radiation were between 6.9-36.7 urad/h for the reactor area and 6.8-19.2 urad/h for the nuclear power plant area

  10. Kepatuhan Nahkoda Melakukan Pemeriksaan Boat Berdasarkan Check- List Harian untuk Keselamatan Pelayaran di Perusahaan Pelayaran

    OpenAIRE

    B, Rinto; Shaluhiyah, Zahroh; Kurniawan, Bina

    2012-01-01

    Terdapat penyimpangan prosedur dalam pemeriksaan boat berdasarkan checklist inspeksiyang sudah ada dimana rata-rata nahkoda melakukan pencontrengan checklist tanpamelakukan uji coba alat-alat keselamatan dan mesin di dalam boat. Tujuan penelitian iniadalah untuk mengeksplorasi gambaran tentang perilaku kepatuhan nahkoda di Perusahaanpelayaran ini dalam melakukan pemeriksaan boat berdasarkan checklist. Penelitian inimenggunakan metode penelitian kualitatif dengan melakukan wawancara yang menda...

  11. Web-Enabled ATR/Fusion Development System

    National Research Council Canada - National Science Library

    Ruda, Harald

    2001-01-01

    .... We have designed a Web-Enabled ATR/Fusion Development System (WEADS) that will allow distributed development and execution of AIR and fusion algorithms using currently available infrastructures...

  12. ATR kinase regulates its attenuation via PPM1D phosphatase ...

    Indian Academy of Sciences (India)

    Debadrita Bhattacharya

    2018-02-07

    Feb 7, 2018 ... generated in response to ultraviolet and ionizing radiation (Lu et al. 2005a, b; Cha ... nocopy' each other's effects by uncovering persistent ATR signalling that in ...... been shown to indirectly stabilize PPM1D by mediating the.

  13. ATR National Scientific User Facility 2009 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Todd R. Allen; Mitchell K. Meyer; Frances Marshall; Mary Catherine Thelen; Jeff Benson

    2010-11-01

    This report describes activities of the ATR NSUF from FY-2008 through FY-2009 and includes information on partner facilities, calls for proposals, users week and education programs. The report also contains project information on university research projects that were awarded by ATR NSUF in the fiscal years 2008 & 2009. This research is university-proposed researcher under a user facility agreement. All intellectual property from these experiments belongs to the university per the user agreement.

  14. ATR National Scientific User Facility 2009 Annual Report

    International Nuclear Information System (INIS)

    Allen, Todd R.; Meyer, Mitchell K.; Marshall, Frances; Thelen, Mary Catherine; Benson, Jeff

    2010-01-01

    This report describes activities of the ATR NSUF from FY-2008 through FY-2009 and includes information on partner facilities, calls for proposals, users week and education programs. The report also contains project information on university research projects that were awarded by ATR NSUF in the fiscal years 2008 and 2009. This research is university-proposed researcher under a user facility agreement. All intellectual property from these experiments belongs to the university per the user agreement.

  15. ATR-dependent bystander effects in non-targeted cells

    International Nuclear Information System (INIS)

    Burdak-Rothkamm, S.

    2007-01-01

    Complete text of publication follows. Radiation induced non-targeted bystander effects have been reported for a range of endpoints including the induction of γH2AX foci which serve as a marker for DNA double strand breaks. We have recently reported the induction of γH2AX foci in non-targeted bystander cells up to 48 hours after irradiation and the involvement of reactive oxygen species (ROS) and TGF-beta 1 in the induction of γH2AX foci (Oncogene (2007) 26:993-1002). Here, we wanted to determine the role of the PI3-like kinases ATM, ATR and DNA-PK in DNA damage signalling in bystander cells. Conditioned medium from T98G cells irradiated with 2 Gy of X-rays was transferred onto non-irradiated cells that were subsequently analysed for the induction of γH2AX, ATR and 53BP1 foci as well as clonogenic survival. Irradiated T98G glioma cells generated signals that induced γH2AX and 53BP1 foci in cells treated with the conditioned medium from irradiated cells. These foci co-localised with ATR foci. Inhibition of ATM and DNA-PK could not suppress the induction of bystander γH2AX foci whereas the mutation of ATR in Seckel cells abrogated bystander foci induction. A restriction of bystander foci to the S-phase of the cell cycle both in T98G cells and in ATR- proficient fibroblasts was observed. These results identify ATR as a central player within the bystander signalling cascade leading to γH2AX and 53BP1 foci formation, and suggest a mechanism of DNA damage induction in non-targeted cells. Further investigations have shown decreased clonogenic cell survival in bystander T98G and ATR wild-type fibroblasts. ATR mutated Seckel cells and also ATM-/- fibroblasts were resistant to this effect suggesting a role for both ATR and ATM in the bystander signalling cascade with regard to cell survival. Taken together, these observations support a hypothesis of DNA damage-induced accumulation of stalled replication forks in bystander cells which are subsequently processed by

  16. Assessment of COTS IR image simulation tools for ATR development

    Science.gov (United States)

    Seidel, Heiko; Stahl, Christoph; Bjerkeli, Frode; Skaaren-Fystro, Paal

    2005-05-01

    Following the tendency of increased use of imaging sensors in military aircraft, future fighter pilots will need onboard artificial intelligence e.g. ATR for aiding them in image interpretation and target designation. The European Aeronautic Defence and Space Company (EADS) in Germany has developed an advanced method for automatic target recognition (ATR) which is based on adaptive neural networks. This ATR method can assist the crew of military aircraft like the Eurofighter in sensor image monitoring and thereby reduce the workload in the cockpit and increase the mission efficiency. The EADS ATR approach can be adapted for imagery of visual, infrared and SAR sensors because of the training-based classifiers of the ATR method. For the optimal adaptation of these classifiers they have to be trained with appropriate and sufficient image data. The training images must show the target objects from different aspect angles, ranges, environmental conditions, etc. Incomplete training sets lead to a degradation of classifier performance. Additionally, ground truth information i.e. scenario conditions like class type and position of targets is necessary for the optimal adaptation of the ATR method. In Summer 2003, EADS started a cooperation with Kongsberg Defence & Aerospace (KDA) from Norway. The EADS/KDA approach is to provide additional image data sets for training-based ATR through IR image simulation. The joint study aims to investigate the benefits of enhancing incomplete training sets for classifier adaptation by simulated synthetic imagery. EADS/KDA identified the requirements of a commercial-off-the-shelf IR simulation tool capable of delivering appropriate synthetic imagery for ATR development. A market study of available IR simulation tools and suppliers was performed. After that the most promising tool was benchmarked according to several criteria e.g. thermal emission model, sensor model, targets model, non-radiometric image features etc., resulting in a

  17. BENTUK PERILAKU SIBLING RIVALRY PADA ANAK KEMBAR BERDASARKAN PENGASUHAN ORANGTUA

    Directory of Open Access Journals (Sweden)

    Herdian Herdian

    2014-07-01

    Full Text Available Penelitian ini bertujuan untuk menggambarkan bentuk perilaku sibling rivalry pada anak kembar berdasarkan pengasuhan orangtua yang terjadi. Penelitian ini menggunakan metode kualitatif, yang pengambilan datanya menggunakan wawancara serta observasi.Subjek penelitiannya adalah 4 pasang anak kembar dan 8 orang terdekat dengan subjek.Validitas data menggunakan teknik triangulasi data dengan sumber, yang berarti membandingkan dan mengecek balik derajat kepercayaan suatu informasi yang diperoleh melalui waktu dan alat berbeda.Analisis data dilakukan dengan bentuk interaktif.Temuan penelitiannya adalah, perilaku sibling rivalry terjadi karena perlakuan orangtua yang berbeda pada masing-masing anak kembar, selain itu dikarenakan cara orangtua dalam memperlakukan anak dalam menangani konflik yang terjadi pada anak kembar. Terdapat dua subjek yang mendapatkan perlakuan yang berbeda ketika berkelahi yaitu dengan membela salah satu anak kembar dikarenakan posisinya sebagai adik.Perlakuan yang berbeda lainnya terlihat pada salah satu subjek yaitu dengan memberikan uang saku yang berbeda. Penyebab sibling rivalry pada anak kembar lainnya hanya disebabkan oleh rebutan barang, baju yang tertukar serta pemberian barang oleh lingkungan sosial yang masing-masing subjek tidak mendapatkan barang yang sama. Bentuk perilaku sibling rivalry yangterjadi terbagi menjadi tiga aspek yaitu fisik, psikis, dan sosial.Aspek fisik bentuk perilakunya yaitu memukul, menendang, mencakar, menjambak, mencubit, dan meninju tembok.Aspek psikis bentuk perilakunya yaitu menangis, cemburu, rewel, iri, sebal, marah, dan usaha mencari perhatian dengan bersandar ke ibu serta berhias.Aspek sosial bentuk perilakunya yaitu mengganggu, mengejek dan bertengkar.Dari hasil tersebut dapat dikategorikan berdasarkan jenis kelamin, laki-laki yaitu meninju tembok, sedangkan perempuan menjambak, mencubit, usaha untuk mencari perhatian dengan bersandar ke ibu serta berhias. Kata kunci : Sibling Rivalry

  18. FLUX SENSOR EVALUATIONS AT THE ATR CRITICAL FACILITY

    International Nuclear Information System (INIS)

    Unruh, Troy; Rempe, Joy; Nigg, David; Imel, George; Harris, Jason; Bonebrake, Eric

    2010-01-01

    The Advanced Test Reactor (ATR) and the ATR Critical (ATRC) facilities lack real-time methods for detecting thermal neutron flux and fission reaction rates for irradiation capsules. Direct measurements of the actual power deposited into a test are now possible without resorting to complicated correction factors. In addition, it is possible to directly measure minor actinide fission reaction rates and to provide time-dependent monitoring of the fission reaction rate or fast/thermal flux during transient testing. A joint Idaho State University/Idaho National Laboratory ATR National Scientific User Facility (ATR NSUF) project was recently initiated to evaluate new real-time state-of-the-art in-pile flux detection sensors. Initially, the project is comparing the accuracy, response time, and long duration performance of French Atomic Energy Commission (CEA)-developed miniature fission chambers, specialized self-powered neutron detectors (SPNDs) by the Argentinean National Energy Commission (CNEA), specially developed commercial SPNDs, and back-to-back fission (BTB) chambers developed by Argonne National Laboratory (ANL). As discussed in this paper, specialized fixturing and software was developed by INL to facilitate these joint ISU/INL evaluations. Calculations were performed by ISU to assess the performance of and reduce uncertainties in flux detection sensors and compare data obtained from these sensors with existing integral methods employed at the ATRC. Ultimately, project results will be used to select the detector that can provide the best online regional ATRC power measurement. It is anticipated that project results may offer the potential to increase the ATRC's current power limit and its ability to perform low-level irradiation experiments. In addition, results from this effort will provide insights about the viability of using these detectors in the ATR. Hence, this effort complements current activities to improve ATR software tools, computational protocols

  19. Homeostatic regulation of meiotic DSB formation by ATM/ATR

    International Nuclear Information System (INIS)

    Cooper, Tim J.; Wardell, Kayleigh; Garcia, Valerie; Neale, Matthew J.

    2014-01-01

    Ataxia–telangiectasia mutated (ATM) and RAD3-related (ATR) are widely known as being central players in the mitotic DNA damage response (DDR), mounting responses to DNA double-strand breaks (DSBs) and single-stranded DNA (ssDNA) respectively. The DDR signalling cascade couples cell cycle control to damage-sensing and repair processes in order to prevent untimely cell cycle progression while damage still persists [1]. Both ATM/ATR are, however, also emerging as essential factors in the process of meiosis; a specialised cell cycle programme responsible for the formation of haploid gametes via two sequential nuclear divisions. Central to achieving accurate meiotic chromosome segregation is the introduction of numerous DSBs spread across the genome by the evolutionarily conserved enzyme, Spo11. This review seeks to explore and address how cells utilise ATM/ATR pathways to regulate Spo11-DSB formation, establish DSB homeostasis and ensure meiosis is completed unperturbed

  20. Homeostatic regulation of meiotic DSB formation by ATM/ATR

    Energy Technology Data Exchange (ETDEWEB)

    Cooper, Tim J.; Wardell, Kayleigh; Garcia, Valerie; Neale, Matthew J., E-mail: m.neale@sussex.ac.uk

    2014-11-15

    Ataxia–telangiectasia mutated (ATM) and RAD3-related (ATR) are widely known as being central players in the mitotic DNA damage response (DDR), mounting responses to DNA double-strand breaks (DSBs) and single-stranded DNA (ssDNA) respectively. The DDR signalling cascade couples cell cycle control to damage-sensing and repair processes in order to prevent untimely cell cycle progression while damage still persists [1]. Both ATM/ATR are, however, also emerging as essential factors in the process of meiosis; a specialised cell cycle programme responsible for the formation of haploid gametes via two sequential nuclear divisions. Central to achieving accurate meiotic chromosome segregation is the introduction of numerous DSBs spread across the genome by the evolutionarily conserved enzyme, Spo11. This review seeks to explore and address how cells utilise ATM/ATR pathways to regulate Spo11-DSB formation, establish DSB homeostasis and ensure meiosis is completed unperturbed.

  1. Grayscale optical correlator for real-time onboard ATR

    Science.gov (United States)

    Chao, Tien-Hsin; Zhou, Hanying; Reyes, George F.

    2001-03-01

    Jet Propulsion Laboratory has been developing grayscale optical correlator (GOC) for a variety of automatic target recognition (ATR) applications. As reported in previous papers, a 128 X 128 camcorder-sized GOC has been demonstrated for real-time field ATR demos. In this paper, we will report the recent development of a prototype 512 X 512 GOC utilizing a new miniature ferroelectric liquid crystal spatial light modulator with a 7-micrometers pixel pitch. Experimental demonstration of ATR applications using this new GOC will be presented. The potential of developing a matchbox-sized GOC will also be discussed. A new application of synthesizing new complex-valued correlation filters using this real-axis 512 X 512 SLM will also be included.

  2. ATR, Radiation Transport Models in Atmosphere at Various Altitudes

    International Nuclear Information System (INIS)

    1981-01-01

    1 - Description of problem or function: ATR is a user-oriented code for calculating quickly and simply radiation environment problems at all altitudes in the atmosphere. The code is based on parametric models of a comprehensive data base of air transport results which were generated using discrete ordinates transport techniques for infinite homogeneous air. The effects of air-ground interface and non-uniform air density are treated as perturbation corrections on homogeneous air results. ATR includes parametric models for neutrons and secondary gamma rays as a function of space, energy and source- target angle out to angles of 550 g/cm 2 of air. ATR contains parameterizations of infinite medium air transport of neutrons and secondary gamma rays and correction factors for the air-ground interface and high altitude exponential air. It responds to a series of user-oriented commands which specify the source, geometry and print options to output a variety of useful air transport information, including energy-angle dependent fluence, dose, current, and isodose ranges. 2 - Method of solution: The version 3 differs from earlier versions in that version 3 contains the parameterization of the new neutron and secondary gamma rays data base that was calculated using the latest DNA approved cross sections for air. Other improvements to the ATR code include: parameterization and inclusion into ATR of new air- over-ground correction factors, low energy x-rays calculations, new fission source, and new convenience options. 3 - Restrictions on the complexity of the problem: ATR takes approximately 36,000 decimal words of storage. This can be lessened by overlaying different parts of the code

  3. Efficacy of ATR inhibitors as single agents in Ewing sarcoma

    DEFF Research Database (Denmark)

    Nieto-Soler, Maria; Morgado-Palacin, Isabel; Lafarga, Vanesa

    2016-01-01

    Ewing sarcomas (ES) are pediatric bone tumors that arise from a driver translocation, most frequently EWS/FLI1. Current ES treatment involves DNA damaging agents, yet the basis for the sensitivity to these therapies remains unknown. Oncogene-induced replication stress (RS) is a known source...... efficacy in ES xenografts as single agents. Expression of EWS/FLI1 or EWS/ERG oncogenic translocations sensitizes non-ES cells to ATR inhibitors. Our data shed light onto the sensitivity of ES to genotoxic agents, and identify ATR inhibitors as a potential therapy for Ewing Sarcomas....

  4. SISTEM PENDUKUNG KEPUTUSAN UNTUK PEMBORAN SUMUR MINYAK BERDASARKAN PERHITUNGAN EKONOMI

    Directory of Open Access Journals (Sweden)

    Herry Sofyan

    2010-01-01

    Full Text Available Oil represent one of nature resource which is not new but represent the solid industry of capital, technological solid and have the high risk. This matter need system of decision supporter capable to give the economics analysis needed by taker of decision or decision maker to analyse the prospect of development economics well of oil drilling so that can be made by a decision base of whether the oil well competent developed or not. Intention of this system is to give the amenity for user in taking decision of whether an developed competent oil well or not. Hence from that felt important to be designed and develop by system of decision supporter for the drilling of oilwell pursuant to economic calculation. In this system economics parameter used in the form of Net Present Value ( NPV, Rate Of Return ( ROR, Profit To Investment Ratio ( PEAR, and Pay Out Time ( POT. Methodologies used in develop this system method of Water Fall and this system will be develop to use the software Delphi 7.0. This system will give the output in the form of decision base whether competent oil well drilling project to be developed or not pursuant to its economics calculation. Minyak merupakan salah satu sumber daya alam yang tidak terbaharukan namun merupakan industri yang padat modal, padat teknologi dan mempunyai resiko yang tinggi. Hal ini memerlukan suatu sistem pendukung keputusan yang mampu memberikan analisa ekonomi yang diperlukan oleh pengambil keputusan atau decision maker untuk menganalisa prospek keekonomian pengembangan suatu sumur pemboran minyak sehingga dapat dijadikan dasar keputusan apakah sumur minyak tersebut layak dikembangkan atau tidak. Tujuan dari sistem ini adalah untuk memberikan kemudahan bagi user dalam mengambil keputusan apakah suatu sumur minyak layak dikembangkan atau tidak. Maka dari itu dirasa penting untuk dirancang dan dibangun sistem pendukung keputusan untuk pemboran sumur minyak berdasarkan perhitungan ekonomi. Dalam sistem ini

  5. PEMILIHAN KONTRASEPSI BERDASARKAN EFEK SAMPING PADA DUA KELOMPOK USIA REPRODUKSI

    Directory of Open Access Journals (Sweden)

    Erna Setiawati

    2017-07-01

    Full Text Available ABSTRACT Kelompok usia reproduksi terbagi dalam tiga fase yaitufase menunda kehamilan (30 tahun. Cara yang ditempuh yaitu dengan pemakaian kontrasepsi.baik  MKJPmaupunnon MKJP. Tujuan penelitian ini adalah untuk mengetahui ada atau tidak perbedaan pemilihan kontrasepsi MKJP dan non MKJP berdasarkan efek samping pada dua kelompok usia reproduksi. Penelitin ini menggunakan desain cross sectional, pengambilan data dengan kuesioner. Sampel dalam penelitian ini adalah akseptor KB baik MKJP maupun non MKJP pada bulan april sampai juni sebanyak 200 responden, dimana tekhnik pengambilan datanya dengan random sampling dan kuota sampling. Hasil penelitian kemudian diuji dengan mann-whitney test.Hasil penelitian dengan uji mann whitney test diperoleh p = 0.662 dengan kata lain p > α (0.05 yang berarti tidak ada perbedaan pemilihan MKJP dan non MKJP berdasarkan efek samping di Wilayah Kabupaten Semarang.      ABSTRACT Reproductive-age category can be divided into three groups which are the group of delayed interval pregnancy (less than 20 years old, the group of intervalcontrol pregnancy (20 to 30 years old, and the group of high risk pregnancy (more than 30 years old. An alternative to avoid high risk pregnancy is by using contraception tool namely long-term contraception (MKJP and non long-term contraception (non MKJP.The purpose of this research is to analysedwhether there are differences in choosing MKJP and non –MKJP based on side effects in the two reproductive-age groups.This research was an explanatory research with cross-sectional design. The population were all women of contraception acceptors in Semarang Regency.The samples were 200 respondents, used simple random sampling and quota sampling. This research used quisionaire instrument and analyze used mann whitney test (α=0,05. Theresult showed thatP = 0,662 meaning P > α = 0.05 which means there is no difference in choosing MKJP and non-MKJP based on side effects in the two reproduction

  6. An ATR architecture for algorithm development and testing

    Science.gov (United States)

    Breivik, Gøril M.; Løkken, Kristin H.; Brattli, Alvin; Palm, Hans C.; Haavardsholm, Trym

    2013-05-01

    A research platform with four cameras in the infrared and visible spectral domains is under development at the Norwegian Defence Research Establishment (FFI). The platform will be mounted on a high-speed jet aircraft and will primarily be used for image acquisition and for development and test of automatic target recognition (ATR) algorithms. The sensors on board produce large amounts of data, the algorithms can be computationally intensive and the data processing is complex. This puts great demands on the system architecture; it has to run in real-time and at the same time be suitable for algorithm development. In this paper we present an architecture for ATR systems that is designed to be exible, generic and efficient. The architecture is module based so that certain parts, e.g. specific ATR algorithms, can be exchanged without affecting the rest of the system. The modules are generic and can be used in various ATR system configurations. A software framework in C++ that handles large data ows in non-linear pipelines is used for implementation. The framework exploits several levels of parallelism and lets the hardware processing capacity be fully utilised. The ATR system is under development and has reached a first level that can be used for segmentation algorithm development and testing. The implemented system consists of several modules, and although their content is still limited, the segmentation module includes two different segmentation algorithms that can be easily exchanged. We demonstrate the system by applying the two segmentation algorithms to infrared images from sea trial recordings.

  7. Skrining Sistematik terhadap Hiperkolesterolemia Familial pada Anak Berdasarkan Kriteria MedPed, Simon Brome Register Register dan Dutch Lipid Clinic

    Directory of Open Access Journals (Sweden)

    Titis Prawitasari

    2016-11-01

    Kesimpulan. Kriteria Dutch Lipid Clinicdapat lebih banyak mendeteksi kemungkinan anak yang mengalami HF berdasarkan riwayat orangtua mengalami penyakit jantung koroner dini dan hiperkolesterolemia dibandingkan kriteria lainnya.

  8. Analisis Karakter Media Pembelajaran Berdasarkan Gaya Belajar Peserta Didik

    Directory of Open Access Journals (Sweden)

    Muhammad Ragil Kurniawan

    2017-05-01

    Full Text Available Abstrak: Penelitian ini bertujuan untuk menganalisis karakter media pembelajaran berdasarkan gaya belajar peserta didik. Jenis penelitian ini adalah penelitian kualitatif. Metode studi kepustakaan digunakan untuk memetakan karakter media pembelajaran yang ditinjau dari gaya belajar peserta didik. Objek yang dianalisis dalam penelitian ini adalah 24 media pembelajaran yang banyak menjadi rujukan pembelajaran. Hasil Penelitian menunjukkan bahwa: Gaya belajar visual dan verbal menjadi gaya belajar yang paling banyak terakomodasi dalam ke-24  media tersebut. Gaya belajar physical hanya terdapat dalam tiga media, yaitu benda nyata, tiruan (model serta pembelajaran berbantuan komputer. Gaya belajar sosial dan individual tidak banyak terakomodasi dalam ke-24 media tersebut. Hal ini karena apapun media pembelajarannya, maka metode, strategi atau model pembelajarnyalah yang memegang peran penting atas terakomodasinya gaya belajar sosial dan individual.   Kata Kunci: karakteristik media, media pembelajaran, gaya belajar, memletic learning style.  Abstrack: This study aims to analyze the character of learning media based on learning styles of learners. This type of research is qualitative research. Literature study method is used to analyze the character of instructional media in terms of learning styles of learners. The object analyzed in this research is 24 learning media which become the reference of learning. Research results show that: Physical learning style is only found in two media: real objects, imitations (models and computer-assisted learning. Social and individual learning styles are only slightly accommodated by those 24 media. This is because any type of learning media used, methods, strategies or learning models become an important role for accommodating of social and individual learning styles.   Keywords: media character, memletic learning styles, instructional media, learning styles

  9. Optimasi Kualitas Akustik Room to Room Berdasarkan Nilai Transmission Loss

    Directory of Open Access Journals (Sweden)

    Fitri Rachmawati

    2013-09-01

    Full Text Available Kamar tidur merupakan salah satu fasilitas utama yang disediakan pada sebuah hotel. Fasilitas ini membutuhkan kenyamanan dan ruangan yang bersifat privasi dimana privasi berkaitan dengan aspek suara yaitu bising (noise. Tingkat kebisingan pada kamar harus diatur dengan adanya insulasi suara agar tidak mengganggu kenyamanan pengunjung saat beristirahat. Nilai insulasi ini dapat digambarkan dengan transmission loss dimana nilai ini dapat diubah menjadi suatu nilai tunggal yang disebut dengan weighted sound reduction index (Rw, DnTw yang mempunyai penambahan faktor koreksi suara frekuensi rendah (C, Ctr. Pada penelitian ini dilakukan insulasi suara pada kamar tidur hotel di Bandara Juanda dengan meninjau aspek dinding partisi untuk menahan kebisingan antar ruangan kamar tidur. Hotel pada penelitian ini merupakan hotel low budget dengan kamar yang minimalis dan ditujukan untuk awak pesawat atau penumpang pesawat yang sedang transit. Walaupun minimalis, tetap diperlukan insulasi suara pada kamar yang baik sehingga dilakukan optimasi dinding partisi dengan ketentuan nilai DnTw+Ctr > 51 dB. Pengukuran pada penelitian ini dilakukan di 5 titik dengan menggunakan SLM RION dan SOLO pada saat malam hari. Berdasarkan hasil pengukuran, diketahui bahwa dinding partisi existing belum memenuhi standar dengan nilai DnTw+Ctr sebesar 35,8 dB sehingga dilakukan perancangan ulang dinding partisi. Perancangan ini menggunakan material gypsum board dan cement board dengan variasi ketebalan material serta cavity yang berbeda. Hasil perancangan yang terbaik yaitu dinding dari material cement board 12,5 mm dengan spesifikasi double panels pada masing-masing sisi dan lebar cavity 70 mm dimana menghasilkan nilai DnTw+Ctr sebesar 52,9 dB. Dengan hasil tersebut, maka dinding partisi sudah memenuhi standar dan mempunyai kualitas insulasi suara yang baik.

  10. STATISTICAL DOWNSCALING DENGAN PERGESERAN WAKTU BERDASARKAN KORELASI SILANG

    Directory of Open Access Journals (Sweden)

    Aji Hamim Wigena

    2015-09-01

    Full Text Available Pergeseran waktu (time lag dalam analisis data deret waktu diperlukan terutama untuk analisis hubungan dua peubah (variable, seperti dalam statistical downscaling. Pergeseran waktu ini ditentukan berdasarkan korelasi silang tinggi yang setara dengan hubungan yang kuat antar kedua peubah tersebut sehingga dapat digunakan dalam pemodelan untuk prakiraan yang lebih akurat. Makalah ini mengenai statistical downscaling dengan memperhatikan korelasi silang antara data curah hujan dengan data presipitasi Global Circulation Model (GCM dari Climate Model Inter Comparison Project (CMIP5. Salah satu syarat dalam statistical downscaling adalah peubah  skala lokal dan global berkorelasi tinggi. Kedua tipe peubah tersebut berupa data deret waktu sehingga fungsi korelasi silang diterapkan untuk memperoleh pergeseran waktu. Korelasi silang yang tinggi menentukan pergeseran waktu pada luaran GCM yang menghasilkan hubungan fungsional lebih kuat antara kedua tipe peubah. Model regresi komponen utama dan regresi kuadrat terkecil parsial digunakan dalam makalah ini. Model-model dengan pergeseran waktu menduga curah hujan lebih baik daripada model-model tanpa pergeseran waktu.   Time lag in time series data analysis is required especially to analyze the relationship of two variables, such as in statistical downscaling. Time lag is determined based on high cross correlation which is equivalent to strong relationship between the two variables and can be used in modeling for a more accurate forecast. This paper is about  statistical downscaling by considering the cross correlation between rainfall data and precipitation data from Global Circulation Model (GCM of Climate Model Inter Comparison Project (CMIP5. One of the conditions in statistical downscaling is that local scale and global scale variables are highly correlated. Both types of variables are time series data, thus cross correlation function is applied to find time lags. High cross correlation determines

  11. Activation of the ATR kinase by the RPA-binding protein ETAA1

    DEFF Research Database (Denmark)

    Haahr, Peter; Hoffmann, Saskia; Tollenaere, Maxim A X

    2016-01-01

    Activation of the ATR kinase following perturbations to DNA replication relies on a complex mechanism involving ATR recruitment to RPA-coated single-stranded DNA via its binding partner ATRIP and stimulation of ATR kinase activity by TopBP1. Here, we discovered an independent ATR activation pathway...... in vertebrates, mediated by the uncharacterized protein ETAA1 (Ewing's tumour-associated antigen 1). Human ETAA1 accumulates at DNA damage sites via dual RPA-binding motifs and promotes replication fork progression and integrity, ATR signalling and cell survival after genotoxic insults. Mechanistically...

  12. Characterization and sintering of niobium-ATR alumina

    International Nuclear Information System (INIS)

    Sibuya, N.H.; Iwasaki, H.; Suzuki, C.K.; Pinatti, D.G.

    1987-01-01

    In the niobium aluminothermy a slag is produced, composed mostly of alumina and other compounds such as niobium oxide and silica. The phase composition of this ATR alumina was characterized by X-ray powder diffractometry, and afterwards this alumina was subjected to leaching processes. It was noticed that the original content of 70% α-alumina in slag rose to 95% after the calcination. ATR alumina (leached and calcined, and without any treatment) was used to make pressed bodies which were fired in air at 1200 to 1400 0 C for 1 to 10,5 hours; and in vacuum at 1550 to 1800$0C for 2 hours. Characterization was done by density measurements, X-ray diffractometry and ultrasonic analysis. Ultrasonic analysis of some vacuum fired bodies showed londitudinal velocities close to the value found in literature. Correlation of several techniques measurements disclosed the niobium oxide interference in sintering. (Author) [pt

  13. Study of radiation exposure rate on the measurement points in Kartini reactor hall as based to determine operation safety parameters (KBO)

    International Nuclear Information System (INIS)

    Mahrus Salam; Elisabeth Supriyatni; Fajar Panuntun

    2016-01-01

    In the operation of nuclear facility there are safety parameters, which is the value of the conservatively maximum limit to ensure that all of the uncertainty in the analysis of facility operations safety have been considered, such as uncertainty of measurement, response time and uncertainty calculation tool, and is get a long to others value of normal operating condition limits, in other words, there are still allowed or permitted. Calculation of the radiation exposure rate on five measurement points (50 cm above the water surface of reactor pool, above interim storage (bulk shielding), reactor deck, thermal column and sub critical facility) and to be compared to the operation safety parameters (KBO) of Kartini reactor. The exposure rate value is obtained by calculating the source term of radioactivity on the core, attenuation resulting from the radiation shielding and measurement distance. From the calculation obtained that the value of gamma exposure rate of 50 cm above the water surface of reactor pool is 96.91 mR/hr (KBO<100 mR/hr), on the deck of Bulk Shielding amounted to 1.70 mR/h (KBO<2.5 mR/hr), on the reactor deck amounted to 5.73 mR/hr (KBO<10 mR/hr), on the Thermal Column amounted to 2.73 mR/hr (KBO<10 mR/hr) and on the sub critical facility amounted to 1.148 mR/hr (KBO<2.5 mR/hr). The value of gamma exposure rate at 5 locations measurements are still less than the operation safety parameters (KBO), it means that the reactor is safe to be operated. (author)

  14. The Neutronic Effect of Zr-rod and Sm2O3-disk in the Model of Kartini Reactor Fuel Element

    International Nuclear Information System (INIS)

    Edi-Trijono-Budisantoso; Bambang-Sumarsono; Tri-Wulan-Tjiptono

    2000-01-01

    The model of Kartini fuel element has been constructed using a variationof materials in it fuels. For basic model, the construction of fuel assemblyis a rod type fuel element with SS-304 cladding and UZrH (8.5 % wt U in 20 %enrichment) as a fuel meat and graphite reflector at the both ends of fuelmeat. By using is basic construction, the material then varied in 4 models asthe following: 1) The model with the Zr-rod in 5.76 mm diameter, is insertedat the axis of fuel meat; 2) The model with Sm 2 O 3 -disk in 1.28 mmthickness, is inserted at the both ends of fuel meat; 3) The compound ofmodel 1 and model 2; 4) The basic construction of cell without Zr-rod andSm 2 O 3 -disk. The neutronic-behavior of each model then calculated usingWIMSD4 and the result then represented as a graphics form of the correlationof parameters as the following: 1) The correlation between model and itneutron multiplication factor; 2) The comparison of neutron flux distributionfor each model. By the graphic can be concluded, that Zr-rod in the fuelelement can reduce power peaking factor to 82 % with the effect of neutronmultiplication factor reduced to 98 %, while the insertion of Sm 2 O 3 -disk atthe both ends of fuel meat can make neutron multiplication factor reduce to97 % but unfortunately the neutron peaking factor increase to 101 %. Theinsertion of Zr-rod in the axis of fuel meat, increase the safety margin offuel utilization, while the insertion of Sm 2 O 3 -disk at the both ends offuel meat can make it as the burn-able poison that maintain the neutronmultiplication factor stable for a long time utilization. (author)

  15. Neutronics analysis of the DHCE experiment in ATR-ITV

    Energy Technology Data Exchange (ETDEWEB)

    Gomes, I.C.; Smith, D.L.; Tsai, H. [Argonne National Lab., IL (United States)

    1997-08-01

    The preliminary analysis of the DHCE experiments in the ITV and ATR was performed and its was concluded that such a vehicle is suitable for this kind of experiment. It is recommended to place an extra filter material in the thermocouple sleeve (such as B-10), to improve the helium to dpa ratio profile during irradiation. Also, it was concluded that a preliminary estimation of period of time for replacement of the external filter would be around 5 dps`s.

  16. Arahan Pengembangan Desa Tertingga Kabupaten Bondowoso Berdasarkan Aspek Sosial, Ekonomi, dan Infrastruktur

    Directory of Open Access Journals (Sweden)

    Amelia Puspasari

    2017-01-01

    Full Text Available Kabupaten Bondowoso merupakan salah satu kabupaten tertinggal di Provinsi Jawa Timur berdasarkan RPJMN tahun 2015-2019. Selain itu, terdapat kesenjangan antara nasional dan kabupaten sehingga perlu dilakukan identifikasi dan pengembangan desa tertinggal di Kabupaten Bondowoso. Permasalahan rendahnya kualitas dari Sumber Daya Manusia (SDM, pelayanan infrastruktur dasar yang belum memadai antara desa satu dengan desa lainnya, dan permasalahan tingginya angka kemiskinan yang terjadi di Kabupaten Bondowoso sehingga untuk mengatasi hal tersebut dilakukan arahan pengembangan desa tertinggal Kabupaten Bondowoso berdasarkan aspek sosial, ekonomi, dan infrastruktur. Penelitian ini bertujuan untuk merumuskan arahan pegembangan desa tertinggal di Kabupaten Bondowoso berdasarkan aspek sosial, ekonomi, dan infrastruktur. Penelitian ini menggunakan teori pegembangan wilayah, pengembangan daerah tertinggal, dan pembangunan perdesaan dengan menggunakan Analisis Faktor Eksplanatory untuk menentukan faktor yang berpengaruh terhadap ketertinggalan desa, Analisis Biplot digunakan untuk tipologi desa tertinggal berdasarkan aspek sosial, ekonomi, serta infrastruktur, dan Analisis Deskripsi digunakan untuk merumuskan arahan pengembangan desa tertinggal berdasarkan aspek-aspek yang diprioritaskan. Dari hasil penelitian terdapat empat faktor yang berpengaruh terhadap ketertinggalan desa di Kabupaten Bondowoso yaitu faktor kualitas SDM, perekonomian masyarakat, kondisi infrastruktur sosial, kondisi infrastruktur ekonomi. Berdasarkan hasil tipologi desa terdapat 95 desa tertinggal di Kabupaten Bondowoso yang terbagi menjadi 3 tipologi. Tipologi A sebanyak 9 desa, Tipologi B sebanyak 59 desa, dan Tipologi C sebanyak 27 desa. Arahan tipologi A peningkatan salah satu aspek SDM dan infrastruktur sosial atau perekonomian dan infrastruktur ekonomi. Arahan tipologi B peningkatan salah satu aspek SDM dan infrastruktur sosial dan atau perekonomian dan infrastruktur ekonomi. Arahan

  17. EKUITAS MEREK JASA GERAI BUAH-BUAHAN BERDASARKAN PERSEPSI KONSUMEN

    Directory of Open Access Journals (Sweden)

    Erdiasa Nursaman

    2015-02-01

    Full Text Available The need of fruits and the number of fruit outlets in Jakarta has been increasing, therefore the study on brand equity of fruit outlet services according to consumer’s perception is now required. In detail the purpose of this study is to analyze the demographic characteristics of  fruits outlets consumer in Jakarta, analyzing the factors that affect brand equity of fruits outlets services, analyze the level of contribution of brand equity variables fruits outlets service in Jakarta and analyze the managerial implications to  fruits outlets brand. Structured questionnaires instrument was used for data collection, while Structural Equation Modeling (SEM method was used in data analysis. The study results have shown that demographic profile of respondents who visited fruit outlet were female, aged 31-40 years, have an undergraduate educational background, private employment and married. Some factors which may influence brand equity are brand loyalty and brand awareness. The brand equity of fruit outlets services is influenced by brand loyalty with contribution of 0.71 and brand awareness with contribution of 0.58. Managerial implication that should be done is to increase promotional activities through advertising. Keywords: brand equity, brand awareness, brand association, brand perceived quality, brand loyaltyABSTRAKLatar belakang dilaksanakannya penelitian ini adalah kebutuhan akan buah-buahan yang semakin meningkat dan makin bertambahnya gerai buah-buahan di Jakarta. Oleh karena itu, diperlukan penelitian ekuitas merek gerai buah-buahan berdasarkan persepsi konsumen. Secara terperinci tujuan penelitian ini menganalisis karakteristik demografi konsumen jasa gerai buah-buahan di Jakarta, menganalisis faktor-faktor yang memengaruhi ekuitas merek jasa layanan gerai buah-buahan, menganalisis tingkat kontribusi peubah-peubah ekuitas merek jasa gerai buah-buahan di Jakarta serta menganalisis implikasi manajerial untuk merek gerai buah-buahan yang

  18. IDENTIFIKASI IKAN SAPU-SAPU (Loricariidae BERDASARKAN KARAKTER POLA ABDOMENDI PERAIRAN CILIWUNG

    Directory of Open Access Journals (Sweden)

    Fatihah Dinul Qoyyimah

    2016-06-01

    Full Text Available Ikan sapu-sapu (Loricariidae merupakan invasive species yang terdapat pada beberapa negara, salah satunyaIndonesia. Ikan tersebut dapat ditemukan pada sungai Ciliwung. Bagian hilir dari sungai tersebut adalah Kota Jakarta.Ikan sapu-sapu yang terdapat di Indonesia termasuk pada Genus Pterygoplichthys. Genus Pterygoplichthys masihsulit dibedakan berdasarkan karakter morfologi. Salah satu cara yang dapat dilakukan untuk mengidentifikasi ikantersebut adalah melihat pola abdomen. Sampel yang digunakan berjumlah 28 ekor. Identifikasi berdasarkan polaabdomen yang dapat dilakukan dengan melihat literatur yang ada. Hasil penelitian yang dilakukan menunjukkanterdapat 2 spesies ikan sapu-sapu pada sungai Ciliwung dan jenis inter-grade. Spesies tersebut adalah Pterygoplichthyspardalis dan Pterygoplichthys disjunctivus.

  19. IDENTIFIKASI IKAN SAPU-SAPU (Loricariidae BERDASARKAN KARAKTER POLA ABDOMENDI PERAIRAN CILIWUNG

    Directory of Open Access Journals (Sweden)

    Fatihah Dinul Qoyyimah

    2016-11-01

    Full Text Available Ikan sapu-sapu (Loricariidae merupakan invasive species yang terdapat pada beberapa negara, salah satunyaIndonesia. Ikan tersebut dapat ditemukan pada sungai Ciliwung. Bagian hilir dari sungai tersebut adalah Kota Jakarta.Ikan sapu-sapu yang terdapat di Indonesia termasuk pada Genus Pterygoplichthys. Genus Pterygoplichthys masihsulit dibedakan berdasarkan karakter morfologi. Salah satu cara yang dapat dilakukan untuk mengidentifikasi ikantersebut adalah melihat pola abdomen. Sampel yang digunakan berjumlah 28 ekor. Identifikasi berdasarkan polaabdomen yang dapat dilakukan dengan melihat literatur yang ada. Hasil penelitian yang dilakukan menunjukkanterdapat 2 spesies ikan sapu-sapu pada sungai Ciliwung dan jenis inter-grade. Spesies tersebut adalah Pterygoplichthyspardalis dan Pterygoplichthys disjunctivus.

  20. A mitosis-specific and R loop-driven ATR pathway promotes faithful chromosome segregation.

    Science.gov (United States)

    Kabeche, Lilian; Nguyen, Hai Dang; Buisson, Rémi; Zou, Lee

    2018-01-05

    The ataxia telangiectasia mutated and Rad3-related (ATR) kinase is crucial for DNA damage and replication stress responses. Here, we describe an unexpected role of ATR in mitosis. Acute inhibition or degradation of ATR in mitosis induces whole-chromosome missegregation. The effect of ATR ablation is not due to altered cyclin-dependent kinase 1 (CDK1) activity, DNA damage responses, or unscheduled DNA synthesis but to loss of an ATR function at centromeres. In mitosis, ATR localizes to centromeres through Aurora A-regulated association with centromere protein F (CENP-F), allowing ATR to engage replication protein A (RPA)-coated centromeric R loops. As ATR is activated at centromeres, it stimulates Aurora B through Chk1, preventing formation of lagging chromosomes. Thus, a mitosis-specific and R loop-driven ATR pathway acts at centromeres to promote faithful chromosome segregation, revealing functions of R loops and ATR in suppressing chromosome instability. Copyright © 2018, American Association for the Advancement of Science.

  1. Status report of the ATR SPING-3A and detailed calibration guideline

    International Nuclear Information System (INIS)

    Koeppen, L.D.; Rogers, J.W.

    1985-12-01

    The Radiation Measurements Laboratory (RML) onducted testing and an evaluation of the ATR SPING-3 after installation of the new ''A'' series software (firmware) and retrofit kit. The purpose of this report is to provide the ATR SPING-3A users with a document describing the present status of the unit, how the system was tested, a suggested calibration guideline, how the system should be operated for the ATR application, and why it should be used in this manner

  2. Analysis of the ATR fuel element swaging process

    International Nuclear Information System (INIS)

    Richins, W.D.; Miller, G.K.

    1995-12-01

    This report documents a detailed evaluation of the swaging process used to connect fuel plates to side plates in Advanced Test Reactor (ATR) fuel elements. The swaging is a mechanical process that begins with fitting a fuel plate into grooves in the side plates. Once a fuel plate is positioned, a lip on each of two side plate grooves is pressed into the fuel plate using swaging wheels to form the joints. Each connection must have a specified strength (measured in terms, of a pullout force capacity) to assure that these joints do not fail during reactor operation. The purpose of this study is to analyze the swaging process and associated procedural controls, and to provide recommendations to assure that the manufacturing process produces swaged connections that meet the minimum strength requirement. The current fuel element manufacturer, Babcock and Wilcox (B ampersand W) of Lynchburg, Virginia, follows established procedures that include quality inspections and process controls in swaging these connections. The procedures have been approved by Lockheed Martin Idaho Technologies and are designed to assure repeatability of the process and structural integrity of each joint. Prior to July 1994, ATR fuel elements were placed in the Hydraulic Test Facility (HTF) at the Idaho National Engineering Laboratory (AGNAIL), Test Reactor Area (TRA) for application of Boehmite (an aluminum oxide) film and for checking structural integrity before placement of the elements into the ATR. The results presented in this report demonstrate that the pullout strength of the swaged connections is assured by the current manufacturing process (with several recommended enhancements) without the need for- testing each element in the HTF

  3. Reconstitution of RPA-covered single-stranded DNA-activated ATR-Chk1 signaling.

    Science.gov (United States)

    Choi, Jun-Hyuk; Lindsey-Boltz, Laura A; Kemp, Michael; Mason, Aaron C; Wold, Marc S; Sancar, Aziz

    2010-08-03

    ATR kinase is a critical upstream regulator of the checkpoint response to various forms of DNA damage. Previous studies have shown that ATR is recruited via its binding partner ATR-interacting protein (ATRIP) to replication protein A (RPA)-covered single-stranded DNA (RPA-ssDNA) generated at sites of DNA damage where ATR is then activated by TopBP1 to phosphorylate downstream targets including the Chk1 signal transducing kinase. However, this critical feature of the human ATR-initiated DNA damage checkpoint signaling has not been demonstrated in a defined system. Here we describe an in vitro checkpoint system in which RPA-ssDNA and TopBP1 are essential for phosphorylation of Chk1 by the purified ATR-ATRIP complex. Checkpoint defective RPA mutants fail to activate ATR kinase in this system, supporting the conclusion that this system is a faithful representation of the in vivo reaction. Interestingly, we find that an alternative form of RPA (aRPA), which does not support DNA replication, can substitute for the checkpoint function of RPA in vitro, thus revealing a potential role for aRPA in the activation of ATR kinase. We also find that TopBP1 is recruited to RPA-ssDNA in a manner dependent on ATRIP and that the N terminus of TopBP1 is required for efficient recruitment and activation of ATR kinase.

  4. Requirement of ATR for maintenance of intestinal stem cells in aging Drosophila.

    Science.gov (United States)

    Park, Joung-Sun; Na, Hyun-Jin; Pyo, Jung-Hoon; Jeon, Ho-Jun; Kim, Young-Shin; Yoo, Mi-Ae

    2015-05-01

    The stem cell genomic stability forms the basis for robust tissue homeostasis, particularly in high-turnover tissues. For the genomic stability, DNA damage response (DDR) is essential. This study was focused on the role of two major DDR-related factors, ataxia telangiectasia-mutated (ATM) and ATM- and RAD3-related (ATR) kinases, in the maintenance of intestinal stem cells (ISCs) in the adultDrosophila midgut. We explored the role of ATM and ATR, utilizing immunostaining with an anti-pS/TQ antibody as an indicator of ATM/ATR activation, γ-irradiation as a DNA damage inducer, and the UAS/GAL4 system for cell type-specific knockdown of ATM, ATR, or both during adulthood. The results showed that the pS/TQ signals got stronger with age and after oxidative stress. The pS/TQ signals were found to be more dependent on ATR rather than on ATM in ISCs/enteroblasts (EBs). Furthermore, an ISC/EB-specific knockdown of ATR, ATM, or both decreased the number of ISCs and oxidative stress-induced ISC proliferation. The phenotypic changes that were caused by the ATR knockdown were more pronounced than those caused by the ATM knockdown; however, our data indicate that ATR and ATM are both needed for ISC maintenance and proliferation; ATR seems to play a bigger role than does ATM.

  5. Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection

    DEFF Research Database (Denmark)

    Carson, Christian T; Orazio, Nicole I; Lee, Darwin V

    2009-01-01

    The protein kinases ataxia-telangiectasia mutated (ATM) and ATM-Rad3 related (ATR) are activated in response to DNA damage, genotoxic stress and virus infections. Here we show that during infection with wild-type adenovirus, ATR and its cofactors RPA32, ATRIP and TopBP1 accumulate at viral...... during virus infection, which is independent of Mre11 nuclease activity and recruitment of RPA/ATR/ATRIP/TopBP1. Unlike other damage scenarios, we found that ATM and ATR signaling are not dependent on each other during infection. We identify a region of the viral E4orf3 protein responsible...

  6. Context and Quasi-Invariants in Automatic Target Recognition (ATR) with Synthetic Aperture Radar (SAR) Imagery

    National Research Council Canada - National Science Library

    Binford, Thomas

    2000-01-01

    .... Experiments based on conventional recognition techniques were conducted for comparisons. Study of persistent scattering confirms the feasibility of implementing a SAR ATR system using physical image features...

  7. Monitoring of biofilm growth using ATR-leaky mode spectroscopy

    International Nuclear Information System (INIS)

    Leitz, M.; Franke, H.; Grattan, K.T.V.; Tamachkiarow, A.

    2002-01-01

    An approach to the in situ monitoring of biofilm formation using the technique of ATR-leaky mode spectroscopy is given as an example for the case of Cytophaga. The biofilm growth was studied on an aluminium layer and on a bilayer of the hydrogel agarose on aluminium. This metal was chosen because of its chemical stability in aqueous systems. The spectra obtained have been recorded using a flow cell to contain the suspension and nutrients over a period of several days. In the case considered using a prism surface coated with agarose, the experiments were performed by breeding in an incubator. (author)

  8. Open source tools for ATR development and performance evaluation

    Science.gov (United States)

    Baumann, James M.; Dilsavor, Ronald L.; Stubbles, James; Mossing, John C.

    2002-07-01

    Early in almost every engineering project, a decision must be made about tools; should I buy off-the-shelf tools or should I develop my own. Either choice can involve significant cost and risk. Off-the-shelf tools may be readily available, but they can be expensive to purchase and to maintain licenses, and may not be flexible enough to satisfy all project requirements. On the other hand, developing new tools permits great flexibility, but it can be time- (and budget-) consuming, and the end product still may not work as intended. Open source software has the advantages of both approaches without many of the pitfalls. This paper examines the concept of open source software, including its history, unique culture, and informal yet closely followed conventions. These characteristics influence the quality and quantity of software available, and ultimately its suitability for serious ATR development work. We give an example where Python, an open source scripting language, and OpenEV, a viewing and analysis tool for geospatial data, have been incorporated into ATR performance evaluation projects. While this case highlights the successful use of open source tools, we also offer important insight into risks associated with this approach.

  9. Safety significance of ATR [Advanced Test Reactor] passive safety response attributes

    International Nuclear Information System (INIS)

    Atkinson, S.A.

    1989-01-01

    The Advanced Test Reactor (ATR) at the Idaho National Engineering Laboratory was designed with some passive safety response attributes which contribute to the safety posture of the facility. The three passive safety attributes being evaluated in the paper are: (1) In-core and in-vessel natural convection cooling, (2) a passive heat sink capability of the ATR primary coolant system (PCS) for the transfer of decay power from the uninsulated piping to the confinement, and (3) gravity feed of emergency coolant makeup. The safety significance of the ATR passive safety response attributes is that the reactor can passively respond for most transients, given a reactor scram, to provide adequate decay power removal and a significant time for operator action should the normal active heat removal systems and their backup systems both fail. The ATR Interim Level 1 Probabilistic Risk Assessment (PRA) model ands results were used to evaluate the significance to ATR fuel damage frequency (or probability) of the above three passive response attributes. The results of the evaluation indicate that the first attribute is a major safety characteristic of the ATR. The second attribute has a noticeable but only minor safety significance. The third attribute has no significant influence on the ATR Level 1 PRA because of the diversity and redundancy of the ATR firewater injection system (emergency coolant system). 8 refs., 4 figs., 1 tab

  10. Initial characterization of the ATR [Advanced Test Reactor] Large Gamma Facility

    International Nuclear Information System (INIS)

    Schnitzler, B.G.; Rogers, J.W.

    1986-05-01

    Radiation fields in the ATR Large Gamma Facility test volume are characterized. The preliminary characterization efforts described in this report include total dose rate measurements in the facility, development of a simple methodology for calculating radiation fields from the ATR fuel element power histories, and a comparison of the measured and calculated values

  11. ARF and ATM/ATR cooperate in p53-mediated apoptosis upon oncogenic stress

    International Nuclear Information System (INIS)

    Pauklin, Siim; Kristjuhan, Arnold; Maimets, Toivo; Jaks, Viljar

    2005-01-01

    Induction of apoptosis is pivotal for eliminating cells with damaged DNA or deregulated proliferation. We show that tumor suppressor ARF and ATM/ATR kinase pathways cooperate in the induction of apoptosis in response to elevated expression of c-myc, β-catenin or human papilloma virus E7 oncogenes. Overexpression of oncogenes leads to the formation of phosphorylated H2AX foci, induction of Rad51 protein levels and ATM/ATR-dependent phosphorylation of p53. Inhibition of ATM/ATR kinases abolishes both induction of Rad51 and phosphorylation of p53, and remarkably reduces the level of apoptosis induced by co-expression of oncogenes and ARF. However, the induction of apoptosis is downregulated in p53-/- cells and does not depend on activities of ATM/ATR kinases, indicating that efficient induction of apoptosis by oncogene activation depends on coordinated action of ARF and ATM/ATR pathways in the regulation of p53

  12. STATUS HUKUM PERALIHAN HAK ATAS TANAH YANG DIPEROLEH DARI LELANG BERDASARKAN HAK MENDAHULU NEGARA

    Directory of Open Access Journals (Sweden)

    Kurnia Martini Dwi Putri A.T.

    2017-04-01

    Abstrak Pajak merupakan salah satu pendukung sumber dana terbesar dalam kegiatan pembangunan Indonesia. Pembayaran pajak merupakan kewajiban bagi setiap Subyek Pajak. Mereka akan dikenal memiliki utang kepada Negara setelah mereka tidak membayar pajak. Kewajiban pajak adalah utang yang sangat timbul karena hukum, sehingga memiliki karakteristik didahulukan dari hutang lainnya. Apabila Wajib Pajak tidak membayar pajak mereka, Pemerintah dapat menjual hasil aset yang bernilai tinggi oleh yang berdasarkan Hak Mendahulu Negara. Di Kota Bandar Lampung, pendaftaran tanah judul transfer yang diperoleh dengan lelang berdasarkan Hak Mendahulu Negara terjadi tanah kepemilikan properti pribadi atas nama pribadi milik, yang menjadi pembayaran kewajiban pajak perusahaan, dan juga obyek sengketa warisan. Judul tanah validitas pendaftaran pengalihan kemudian menjadi hukum dipertanyakan, karena objek lelang dicatat atas nama kepemilikan individu, bukan perusahaan. Selain itu, di bawah sengketa warisan dan telah diblokade di Kantor Pertanahan Bandar Lampung.   Kata Kunci: Hak Mendahulu Negara, Lelang, Pemblokiran Sertifikat Tanah

  13. DATA MINING UNTUK MEMPREDIKSI PRESTASI SISWA BERDASARKAN SOSIAL EKONOMI, MOTIVASI, KEDISIPLINAN DAN PRESTASI MASA LALU

    Directory of Open Access Journals (Sweden)

    Heri Susanto

    2014-06-01

    Full Text Available Penelitian ini bertujuan untuk membuat prediksi prestasi belajar siswa berdasarkan status sosial ekonomi orang tua, motivasi, kedisiplinan siswa dan prestasi masa lalu menggunakan metode data mining dengan algoritma J48.  Sebagai perbandingan, data penelitian dianalisis juga dengan CHAID (Chi Squared Automatic Interaction Detection dan regresi ganda. Pendekatan penelitian yang digunakan adalah kuantitatif. Subyek penelitian ini adalah siswa tingkat X SMK Negeri 4 Surakarta berjumlah 416 siswa. Teknik pengumpulan data yang digunakan adalah dokumentasi dan angket. Hasil penelitian menunjukkan bahwa analisis prediksi menggunakan decision tree algoritma J48 memiliki akurasi sebesar 95,7%, sedangkan analisis prediksi menggunakan CHAID memiliki tingat akurasi 82,1% dan analisis regresi ganda menghasilkan tingkat signifikansi sebesar 90,6%. Berdasarkan hasil tersebut bisa disimpulkan bahwa metode J48 lebih baik dibandingkan dengan metode CHAID dan regresi ganda.Kata kunci: data mining

  14. Klasifikasi Kecamatan Berdasarkan Nilai Akhir SMA/MA di Kabupaten Aceh Selatan Menggunakan Analisis Diskriminan

    Directory of Open Access Journals (Sweden)

    Fitriana A.R.

    2015-11-01

    Full Text Available Analisis diskriminan merupakan bagian dari analisis peubah ganda. Analisis diskriminan digunakan untuk memodelkan variabel tak bebas yang bersifat kualitatif dengan variabel bebas yang bersifat kuantitatif. Pada penelitian ini, analisis diskriminan lebih dari dua kelompok diterapkan pada data nilai akhir SMA/MA di Kabupaten Aceh Selatan yang bertujuan untuk mendapatkan model untuk pengklasifikasian kecamatan. Pengklasifikasian ini bertujuan untuk melihat perbedaan kualitas siswa SMA/MA yang berada di masing-masing kecamatan Kabupaten Aceh Selatan berdasarkan data nilai akhir. Variabel tak bebas yang digunakan berupa klasifikasi kecamatan berdasarkan rata-rata jumlah nilai akhir SMA/MA yang berada di masing-masing kecamatan yang dibentuk dengan K-Means Cluster. Sedangkan variabel bebas yang digunakan adalah data rata-rata nilai akhir SMA/MA untuk setiap mata pelajaran yang diujiankan pada masing-masing jurusan setiap kecamatan. Model yang didapatkan adalah dua model diskriminan untuk jurusan IPA dan IPS.

  15. SELEKSI BAKTERI METHANOTROF (PEREDUKSI EMISI GAS METAN DI LAHAN SAWAH BERDASARKAN AKTIVITAS ENZIM METHAN MONOOKSIGENASE

    Directory of Open Access Journals (Sweden)

    Maimuna Nonci

    2016-03-01

    Full Text Available Kehadiran bakteri metanotrof pada daerah rhizosfer padi sangat dibutuhkan untuk mereduksi metan yang dihasilkan oleh bakteri metanogen, sehingga tidak terjadi emisi gas metan ke atmosfer. Penelitian ini bertujuan untuk melakukan koleksi dan seleksi bakteri methanotrof asal rhizosfer tanaman padi yang mampu menghasilkan enzim metan monooksigenase. Pengambilan sampel dilakukan secara acak pada tiga fase pertumbuhan tanaman padi yaitu fase vegetatif, reproduktif dan pematangan. Koleksi isolat dilakukan dengan metode isolasi dan pemurnian. Seleksi isolat dilakukan dengan pengujian reaksi gram, motil, indol, aerob/anaerob dan aktivitas enzim monooksigenase dianalisis dengan metode kolorimetrik.  Hasil penelitian diperoleh 52 koleksi isolat bakteri. Berdasarkan reaksi gram terseleksi 22 isolat gram negatif, berdasarkan analisis kolorimetrik  diperoleh 10 isolat  menghasilkan enzim metan monooksigenase.

  16. SURVEI TEKNIK CLUSTERING ROUTING BERDASARKAN MOBILITAS PADA WIRELESS AD-HOC NETWORK

    Directory of Open Access Journals (Sweden)

    I Nyoman Trisna Wirawan

    2016-01-01

    Full Text Available Wireless ad-hoc merupakan sebuah skema jaringan yang didesain supaya mampu beroperasi tanpa membutuhkan infrastruktur tetap serta bersifat otonom. Teknik flooding pada proses path discovery dalam kasus wireless ad-hoc network dapat menimbulkan masalah beban jaringan yang berlebihan. Oleh karena itu, sebuah skema clustering diusulkan untuk mengurangi adanya flooding paket yang berlebihan dengan membagi node-node dalam jaringan menjadi beberapa bagian berdasarkan parameter tertentu. Teknik ini efektifuntuk mengurangi paket yang harus dilewatkan dalam jaringan. Namun masalah muncul ketika sebuah jaringan wireless ad-hoc harus membentuk sebuah cluster dengan mempertimbangkan beberapa parameter khusus. Parameter tersebut harus disesuaikan dengan kasus yang dihadapi. Pada tulisan ini akan dibahas secara khusus mengenai penerapan skema clustering dalam lingkungan wireless ad-hoc network, baik pada MANETdan penyesuaian skema clustering yang harus dilakukan pada VANET berdasarkan mobilitasnya.

  17. Analisis Hasil Filtering Karous-Hjelt Berdasarkan Beda Spasi Dalam Penggambaran Struktur Bawah Permukaan Tanah

    Directory of Open Access Journals (Sweden)

    Miftakhul Maulidina

    2017-04-01

    Full Text Available Struktur penyusun tanah di setiap wilayah dapat digambarkan melalui pemodelan berdasarkan nilai resistivitas material penyusunnya. Tujuan penelitian ini membuat penggambaran struktur bawah permukaan tanah melalui filtering Karous-Hjelt dengan variasi spasi. Adapun spasi dalam pengambilan data di lapangan adalah spasi 1 meter dan spasi 0,5 meter pada masing-masing line. Penelitian ini sekaligus melengkapi hasil penelitian sebelumnya tentang penentuan struktur bawah permukaan tanah di sekitar Candi Gambar Wetan (Maulidina, 2015. Data yang dimasukkan merupakan data hasil pengukuran Very Low Frequency Electromagnetic di area Candi Gambar Wetan. Metode yang digunakan yaitu pengolahan data menggunakan filtering Karous-Hjelt untuk penggambaran struktur bawah permukaan tanah pada kedalaman 0 – 6 meter untuk dua jenis data berdasarkan variasi spasi. Hasil pengolahan menunjukkan penggambaran struktur bawah permukaan tanah spasi 0,5 m memiliki rentang nilai resistivitas yang lebih mendetail dibandingkan spasi 1 m. Hasil ini dapat menambah informasi dan bisa digunakan sebagai acuan dalam penelitian berikutnya.

  18. ANALISIS SEGMENTASI PASAR PRODUK YOGHURT BERDASARKAN PERILAKU KONSUMEN PADA HYPERMART CABANG PANAKUKANG, MAKASSAR

    OpenAIRE

    -, ANDRIANI

    2014-01-01

    2014 v ABSTRAK Andriani. I311 04 049. Analisis Segmentasi Pasar Berdasarkan Perilaku Konsumen pada Hypermart Cabang Panakukang, Makassar. Dibawah Bimbingan Ir. Hastang, M.Si sebagai Pembimbing Utama dan Muh. Ridwan, S.Pt, M.Si. sebagai Pembimbing Anggota. Perkembangan dan peningkatan bisnis khususnya pada usaha peternakan akhir-akhir ini menunjukkan banyak peningkatan. Salah satu strategi yang dapat diterapkan dalam menunjang aktivitas pemasaran oleh perusahaan dalam memasarkan pr...

  19. KERAGAAN WARNA IKAN CLOWN BIAK (Amphiprion percula) POPULASI ALAM DAN BUDIDAYA BERDASARKAN ANALISIS GAMBAR DIGITAL

    OpenAIRE

    Ruby Vidia Kusumah; Sawung Cindelaras; Anjang Bangun Prasetio

    2015-01-01

    Penelitian ini bertujuan untuk mengkaji keragaan warna ikan clown Biak (Amphiprion percula) populasi alam dan budidaya berdasarkan analisis gambar digital sebagai dasar upaya pemuliaannya. Gambar digital diambil dari koleksi ikan clown Biak Balai Besar Perikanan Budidaya Laut Lampung, Balai Besar Penelitian dan Pengembangan Budidaya Laut Gondol, Bali; serta pengumpul ikan hias di Denpasar, Bali menggunakan kamera digital Canon EOS 600D. Pola warna dikarakterisasi secara visual terhadap varias...

  20. IDENTIFIKASI IKAN SAPU-SAPU (Loricariidae) BERDASARKAN KARAKTER POLA ABDOMENDI PERAIRAN CILIWUNG

    OpenAIRE

    Fatihah Dinul Qoyyimah; Dewi Elfidasari; Melta Rini Fahmi

    2016-01-01

    Ikan sapu-sapu (Loricariidae) merupakan invasive species yang terdapat pada beberapa negara, salah satunyaIndonesia. Ikan tersebut dapat ditemukan pada sungai Ciliwung. Bagian hilir dari sungai tersebut adalah Kota Jakarta.Ikan sapu-sapu yang terdapat di Indonesia termasuk pada Genus Pterygoplichthys. Genus Pterygoplichthys masihsulit dibedakan berdasarkan karakter morfologi. Salah satu cara yang dapat dilakukan untuk mengidentifikasi ikantersebut adalah melihat pola abdomen. Sampel yang digu...

  1. Arahan Pengembangan Desa Tertingga Kabupaten Bondowoso Berdasarkan Aspek Sosial, Ekonomi, dan Infrastruktur

    OpenAIRE

    Puspasari, Amelia; Koswara, Arwi Yudhi

    2016-01-01

    Kabupaten Bondowoso merupakan salah satu kabupaten tertinggal di Provinsi Jawa Timur berdasarkan RPJMN tahun 2015-2019. Selain itu, terdapat kesenjangan antara nasional dan kabupaten sehingga perlu dilakukan identifikasi dan pengembangan desa tertinggal di Kabupaten Bondowoso. Permasalahan rendahnya kualitas dari Sumber Daya Manusia (SDM), pelayanan infrastruktur dasar yang belum memadai antara desa satu dengan desa lainnya, dan permasalahan tingginya angka kemiskinan yang terjadi di Kabupate...

  2. Structural Analysis of Spiropyran Polimers using ATR Spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Delgado Macui, R; Rojas Lopez, M; Gayou, V L; Orduna Diaz, A [Centro de Investigacion en BiotecnologIa Aplicada del IPN, San Juan de los Molinos km. 1.5 Tepetitla de Lardizabal, A.P. 90700, Tlaxcala (Mexico)

    2006-01-01

    We have used infrared spectroscopy in attenuated total reflection (ATR) mode to analyze the interactions between the polymeric base and solvent with a photochromic material (spyropiran). We used cellulose acetate as polymeric base and the spyropiran; 1,3,3 trimethyl indoline-5-nitro benzopyrane. Thin films with different weight concentrations of SP were deposited in the polymeric base. The infrared spectra show bands whose frequencies are associated to several molecular bondings. It was observed a decreasing in intensity of absorbance for C = O stretching mode of the acetate group at 1720 cm{sup -1} and for C = C stretching mode for the main chain at 823 and 982 cm{sup -1} both associated to the presence of SP in polymeric films.

  3. Characterization and sintering of ATR aluminia from niobium

    International Nuclear Information System (INIS)

    Shibuya, N.H.

    1987-01-01

    The characterization of resultante slag from Aluminothermic Reduction (ATR) process to obtain metallic niobium is presented. The slag was characterized for concentration and phases of aluminia by X-ray diffractometry. The results show that 70% of the slag is constituted by α aluminia. The lixiviation and calcination of the slag increased the α aluminia concentration to 95%, the slag was used for producing samples to be burning in three furnaces: electrical resistance furnace in the air, and two furnaces in the vacuum. The burned samples were characterized by microscopy, ultrasonic analysis, density measurements and X-ray diffractometry. The sintering in the vacuum is possible because the samples burned in vacuum presented major density. The formation of NbO 2 and mullite was observed, by X-ray diffractometry. The data from optical microscopy, density measurements and X-ray diffractometry show high porosity. (M.C.K.) [pt

  4. Mutation of serine 1333 in the ATR HEAT repeats creates a hyperactive kinase.

    Directory of Open Access Journals (Sweden)

    Jessica W Luzwick

    Full Text Available Subcellular localization, protein interactions, and post-translational modifications regulate the DNA damage response kinases ATR, ATM, and DNA-PK. During an analysis of putative ATR phosphorylation sites, we found that a single mutation at S1333 creates a hyperactive kinase. In vitro and in cells, mutation of S1333 to alanine (S1333A-ATR causes elevated levels of kinase activity with and without the addition of the protein activator TOPBP1. S1333 mutations to glycine, arginine, or lysine also create a hyperactive kinase, while mutation to aspartic acid decreases ATR activity. S1333A-ATR maintains the G2 checkpoint and promotes completion of DNA replication after transient exposure to replication stress but the less active kinase, S1333D-ATR, has modest defects in both of these functions. While we find no evidence that S1333 is phosphorylated in cultured cells, our data indicate that small changes in the HEAT repeats can have large effects on kinase activity. These mutants may serve as useful tools for future studies of the ATR pathway.

  5. ATM and ATR play complementary roles in the behavior of excitatory and inhibitory vesicle populations.

    Science.gov (United States)

    Cheng, Aifang; Zhao, Teng; Tse, Kai-Hei; Chow, Hei-Man; Cui, Yong; Jiang, Liwen; Du, Shengwang; Loy, Michael M T; Herrup, Karl

    2018-01-09

    ATM (ataxia-telangiectasia mutated) and ATR (ATM and Rad3-related) are large PI3 kinases whose human mutations result in complex syndromes that include a compromised DNA damage response (DDR) and prominent nervous system phenotypes. Both proteins are nuclear-localized in keeping with their DDR functions, yet both are also found in cytoplasm, including on neuronal synaptic vesicles. In ATM- or ATR-deficient neurons, spontaneous vesicle release is reduced, but a drop in ATM or ATR level also slows FM4-64 dye uptake. In keeping with this, both proteins bind to AP-2 complex components as well as to clathrin, suggesting roles in endocytosis and vesicle recycling. The two proteins play complementary roles in the DDR; ATM is engaged in the repair of double-strand breaks, while ATR deals mainly with single-strand damage. Unexpectedly, this complementarity extends to these proteins' synaptic function as well. Superresolution microscopy and coimmunoprecipitation reveal that ATM associates exclusively with excitatory (VGLUT1 + ) vesicles, while ATR associates only with inhibitory (VGAT + ) vesicles. The levels of ATM and ATR respond to each other; when ATM is deficient, ATR levels rise, and vice versa. Finally, blocking NMDA, but not GABA, receptors causes ATM levels to rise while ATR levels respond to GABA, but not NMDA, receptor blockade. Taken together, our data suggest that ATM and ATR are part of the cellular "infrastructure" that maintains the excitatory/inhibitory balance of the nervous system. This idea has important implications for the human diseases resulting from their genetic deficiency.

  6. RPA-Binding Protein ETAA1 Is an ATR Activator Involved in DNA Replication Stress Response.

    Science.gov (United States)

    Lee, Yuan-Cho; Zhou, Qing; Chen, Junjie; Yuan, Jingsong

    2016-12-19

    ETAA1 (Ewing tumor-associated antigen 1), also known as ETAA16, was identified as a tumor-specific antigen in the Ewing family of tumors. However, the biological function of this protein remains unknown. Here, we report the identification of ETAA1 as a DNA replication stress response protein. ETAA1 specifically interacts with RPA (Replication protein A) via two conserved RPA-binding domains and is therefore recruited to stalled replication forks. Interestingly, further analysis of ETAA1 function revealed that ETAA1 participates in the activation of ATR signaling pathway via a conserved ATR-activating domain (AAD) located near its N terminus. Importantly, we demonstrate that both RPA binding and ATR activation are required for ETAA1 function at stalled replication forks to maintain genome stability. Therefore, our data suggest that ETAA1 is a new ATR activator involved in replication checkpoint control. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. Generation and assessment of turntable SAR data for the support of ATR development

    Science.gov (United States)

    Cohen, Marvin N.; Showman, Gregory A.; Sangston, K. James; Sylvester, Vincent B.; Gostin, Lamar; Scheer, C. Ruby

    1998-10-01

    Inverse synthetic aperture radar (ISAR) imaging on a turntable-tower test range permits convenient generation of high resolution two-dimensional images of radar targets under controlled conditions for testing SAR image processing and for supporting automatic target recognition (ATR) algorithm development. However, turntable ISAR images are often obtained under near-field geometries and hence may suffer geometric distortions not present in airborne SAR images. In this paper, turntable data collected at Georgia Tech's Electromagnetic Test Facility are used to begin to assess the utility of two- dimensional ISAR imaging algorithms in forming images to support ATR development. The imaging algorithms considered include a simple 2D discrete Fourier transform (DFT), a 2-D DFT with geometric correction based on image domain resampling, and a computationally-intensive geometric matched filter solution. Images formed with the various algorithms are used to develop ATR templates, which are then compared with an eye toward utilization in an ATR algorithm.

  8. Process computer system for the prototype ATR 'Fugen'

    International Nuclear Information System (INIS)

    Oteru, Shigeru

    1979-01-01

    In recent nuclear power plants, computers are regarded as one of component equipments, and data processing, plant monitoring and performance calculation tend to be carried out with one on-line computer. As plants become large and complex, and the operational conditions become strict, the system having the function of performance calculation and reflecting the results immediately to operation is introduced. In the process computer for the prototype ATR ''Fugen'', the function of prediction to simulate the state after operation before the operation accompanied by the change of reactivity in a core, such as the operation of control rods and the control of liquid poison during operation, was given in addition to the functions of data processing, plant monitoring and detailed performance calculation. Core periodic monitoring program, core operational aid program, core any time data collecting program, and core periodic data collecting program, and their application programs are explained. Core performance calculation is the calculation of thermal output distribution in the core and the various accompanying characteristics and the monitoring of thermal limiting values. The computer used is a Hitachi control computer HIDIC-500, and typewriters, a process colored display, an operating console and other peripheral equipments are connected to it. (Kako, I.)

  9. Encefalomenigocele atrésico parietal Parietal atresic encephalomeningocele

    Directory of Open Access Journals (Sweden)

    Liliana Rivera Oliva

    2011-09-01

    Full Text Available El encefalocele es una anomalía congénita rara, en la que una porción del encéfalo protruye a través de un orificio craneal (evaginación, generalmente situado en la línea media. Clínicamente se caracteriza por una masa epicraneal, de consistencia blanda, muchas veces acompañada de trastornos psicomotores, convulsiones y trastornos de la visión. Se presenta el caso de un recién nacido con diagnóstico de encefalomeningocele atrésico parietal, intervenido quirúrgicamente y con evolución satisfactoria.The encephalocele is a uncommon congenital anomaly where a portion of encephalon protrudes through a cranial orifice (evagination, generally located in the middle line. Clinically, it is characterized by a soft epicranial mass often accompanied or psychomotor disorders, convulsions and vision disorders. This is the case of a newborn diagnosed with parietal atresic encephalomeningocele operated on with a satisfactory evolution.

  10. Functional analyses of ATM, ATR and Fanconi anemia proteins in lung carcinoma

    International Nuclear Information System (INIS)

    Beumer, Jan H.; Fu, Katherine Y.; Anyang, Bean N.; Siegfried, Jill M.; Bakkenist, Christopher J.

    2015-01-01

    ATM and ATR are kinases implicated in a myriad of DNA-damage responses. ATM kinase inhibition radiosensitizes cells and selectively kills cells with Fanconi anemia (FA) gene mutations. ATR kinase inhibition sensitizes cells to agents that induce replication stress and selectively kills cells with ATM and TP53 mutations. ATM mutations and FANCF promoter-methylation are reported in lung carcinomas. We undertook functional analyses of ATM, ATR, Chk1 and FA proteins in lung cancer cell lines. We included Calu6 that is reported to be FANCL-deficient. In addition, the cancer genome atlas (TCGA) database was interrogated for alterations in: 1) ATM, MRE11A, RAD50 and NBN; 2) ATR, ATRIP and TOPBP1; and 3) 15 FA genes. No defects in ATM, ATR or Chk1 kinase activation, or FANCD2 monoubiquitination were identified in the lung cancer cell lines examined, including Calu6, and major alterations in these pathways were not identified in the TCGA database. Cell lines were radiosensitized by ATM kinase inhibitor KU60019, but no cell killing by ATM kinase inhibitor alone was observed. While no synergy between gemcitabine or carboplatin and ATR kinase inhibitor ETP-46464 was observed, synergy between gemcitabine and Chk1 kinase inhibitor UCN-01 was observed in 54 T, 201 T and H460, and synergy between carboplatin and Chk1 kinase inhibitor was identified in 201 T and 239 T. No interactions between ATM, ATR and FA activation were observed by either ATM or ATR kinase inhibition in the lung cancer cell lines. Analyses of ATM serine 1981 and Chk1 serine 345 phosphorylation, and FANCD2 monoubiquitination revealed that ATM and ATR kinase activation and FA pathway signaling are intact in the lung cancer cell lines examined. As such, these posttranslational modifications may have utility as biomarkers for the integrity of DNA damage signaling pathways in lung cancer. Different sensitization profiles between gemcitabine and carboplatin and ATR kinase inhibitor ETP-46464 and Chk1 kinase inhibitor

  11. RENDEMEN TEPUNG BUAH NIPAH (Nyfa fruticans WURMB BERDASARKAN JARAK TEMPAT TUMBUH

    Directory of Open Access Journals (Sweden)

    Fatriani Fatriani

    2016-09-01

    Full Text Available Abstract.  This study aims to determine the magnitude of the yield of wheat grown fruit Nipah by site. Nipah fruit yield of flour was 27.69% submerged in water, slightly submerged in water is 25.00% and grown on land which is 24.73%, Based on the analysis of the growing diversity was no significant effect on the yield of the resulting flour. Factors that influence the magnitude of the yield of fruit flour Nipah is the raw material used, Nipah fruit size, equipment used in the production process, and rigor in the process of flour production Nipah fruit. Based on the results obtained it is necessary to study Nipah fruit yield of flour from various regions, with special techniques and tools in the fruit paring Nipah. Keywords: Nipah, starch, sucrose content ABSTRAK.  Penelitian ini bertujuan untuk mengetahui besarnya rendemen  tepung buah Nipah berdasarkan tempat tumbuh. Rendemen tepung buah Nipah yang terendam air adalah 27,69% ,yang agak terendam air adalah 25,00% dan yang tumbuh di daratan  adalah 24,73%, Berdasarkan analisis keragaman ternyata tempat tumbuh tidak memberikan pengaruh yang nyata terhadap rendemen tepung yang dihasilkan. Faktor-faktor yang mempengaruhi besarnya rendemen tepung buah Nipah adalah bahan baku yang digunakan, ukuran buah Nipah, peralatan yang digunakan dalam proses produksi, dan ketelitian dalam proses produksi tepung buah Nipah. Berdasarkan hasil yang diperoleh maka perlu penelitian rendemen tepung buah Nipah dari berbagai daerah,  dengan teknik dan alat khusus dalam pengupasan buah Nipah. Katakunci : Nipah, tepung, rendemen

  12. PENYUSUNAN SKENARIO MASA TANAM BERDASARKAN PRAKIRAAN CURAH HUJAN DI SENTRA PRODUKSI PANGAN

    Directory of Open Access Journals (Sweden)

    Woro Estiningtyas

    2008-11-01

    Penggunaan model deterministik untuk prediksi curah hujan di daerah tropik yang faktor determinannya sangat komplek, dinamis dan acak sangat rumit. Oleh karena itu diperlukan model statistik yang dapat diperbarui secara real time. Filter Kalman menggabungkan pendekatan model fisik dan statistik menjadi model stokastik yang dapat diperbarui setiap saat untuk tujuan peramalan segera (on line forecasting. Validasi model yang menghubungkan curah hujan dan suhu permukaan laut Nio 3.4 menghasilkan nilai koefisien korelasi lebih dari 75%. Artinya model prediksi dengan Filter Kalman ini dapat digunakan untuk memprakirakan curah hujan bulanan dan diaplikasikan untuk penyusunan masa tanam. Selama ini neraca air tanaman dihitung berdasarkan pola curah hujan setempat, namun dengan meningkatnya intensitas dan frekuensi anomali iklim akan menyebabkan hasil komputasi neraca air harus diperbarui setiap saat melalui penyusunan masa tanam yang memperhitungkan aspek prediksi. Prakiraan curah hujan dengan metode Filter Kalman menghasilkan nilai koefisien korelasi validasi 48-92%. Hasil skenario pola tanam berdasarkan data prediksi curah hujan memperlihatkan bahwa ditemukan periode-periode dengan persentase kehilangan hasil lebih dari 20%, terutama pada lokasi dengan distribusi curah hujan yang tidak merata sepanjang tahun. Dengan demikian tidak disarankan untuk melakukan penanaman pada periode tersebut. Lokasi Sukamandi memperlihatkan karakteristik model yang lebih bagus dibandingkan Tamanbogo, Batang, Wonosari. Tanggal tanam yang diperkirakan beresiko menurunkan hasil adalah 1 dan 11 November (di Tamanbogo, 1 November-1 Januari, dan 21 Februari (di Sukamandi, 1 November-1 Desember (di Batang dan  1 November, 11 dan 21 Februari (di Wonosari. Untuk aplikasi skenario masa tanam, model prakiraan hujan perlu di perbarui di setiap saat dan tempat berdasarkan data prakiraan curah hujan terbaru di dukung dengan data tanah dan tanaman. Selain itu perlu diperhatikan cakupan wilayah yang bisa

  13. PENINGKATAN KEMAMPUAN MAHASISWA DALAM MENGEMBANGKAN PERANGKAT PEMBELAJARAN FISIKA MELALUI PENELAAHAN PERANGKAT PEMBELAJARAN BERDASARKAN MASALAH

    Directory of Open Access Journals (Sweden)

    Hermin Budiningarti

    2015-12-01

    Full Text Available Penelitian ini dilatarbelakangi oleh sejumlah perangkat pembelajaran yang belum dimanfaatkan secara optimal oleh mahasiswa. Melalui perkuliahan Teaching Learning Process 3 (TLP 3 yang berisi pengkajian tentang model-model pembelajaran selama ini disajikan secara teoritis melalui tatap muka, pemodelan, workshop, dan praktek pembelajaran dalam forum, perangkat tersebut dioptimalkan. Standar kompetensi mata kuliah tersebut adalah mahasiswa terampil merancang dan mengelola proses pembelajaran menggunakan model pembelajaran diskusi kelas (class discussion,  penemuan terbimbing (guided discovery, dan pembelajaran berdasarkan masalah (problem based learning. Kompetensi ini akan lebih mudah tercapai bilamana mahasiswa sebelumnya dibekali dengan kemampuan menilai sebuah perangkat pembelajaran terkait kesesuaiannya dengan model pembelajaran yang diterapkan. Pada penelitian ini, perangkat pembelajaran berdasarkan masalah (PBI karya mahasiswa terdahulu dinilai oleh mahasiswa dan dosen dengan menggunakan instrumen yang sama (IPKG1 menghasilkan penilaian X1 dan X2. Setelah itu mahasiswa merancang perangkat pembelajaran model PBI dan dinilai oleh dosen menggunakan IPKG1 dan menghasilkan penilaian Y1. Perangkat pembelajaran PBI yang telah dihasilkan kemudian disimulasikan oleh mahasiswa dan dinilai oleh dosen dengan menggunakan IPKG2 menghasilkan penilaian Y2. Analisis data menggunakan analisis korelasi dan analisis jalur (path analysis. Hasil penelitian menghasilkan beberapa temuan: 1 Tingkat kesesuaian penilaian perangkat PBI oleh mahasiswa dan dosen cukup tinggi dengan korelasi yang tinggi, 2 Keterampilan mahasiswa dalam mengembangkan perangkat pembelajaran model pembelajaran berdasarkan masalah untuk skala 0-100 berada pada kisaran 77,94-97,06 dengan rata-rata 87,04 yang berarti baik. Penilaian contoh perangkat oleh mahasiswa dan dosen tidak berkorelasi dan tidak memberikan dampak atas keterampilan mahasiswa dalam mengembangkan perangkat pembelajaran model

  14. Roles of ATR1 paralogs YMR279c and YOR378w in boron stress tolerance

    International Nuclear Information System (INIS)

    Bozdag, Gonensin Ozan; Uluisik, Irem; Gulculer, Gulce Sila; Karakaya, Huseyin C.; Koc, Ahmet

    2011-01-01

    Highlights: → ATR1 paralog YMR279c plays role in boron detoxification. → YMR279c overexpression lowers cytoplasmic boron levels. → ATR1 paralog YOR378w has no roles in boron stress response. -- Abstract: Boron is a necessary nutrient for plants and animals, however excess of it causes toxicity. Previously, Atr1 and Arabidopsis Bor1 homolog were identified as the boron efflux pump in yeast, which lower the cytosolic boron concentration and help cells to survive in the presence of toxic amount of boron. In this study, we analyzed ATR1 paralogs, YMR279c and YOR378w, to understand whether they participate in boron stress tolerance in yeast. Even though these genes share homology with ATR1, neither their deletion rendered cells boron sensitive nor their expression was significantly upregulated by boron treatment. However, expression of YMR279, but not YOR378w, from the constitutive GAPDH promoter on a high copy plasmid provided remarkable boron resistance by decreasing intracellular boron levels. Thus our results suggest the presence of a third boron exporter, YMR279c, which functions similar to ATR1 and provides boron resistance in yeast.

  15. ANALISIS KEMAMPUAN PEMECAHAN MASALAH MATEMATIKA SISWA KELAS X DALAM PEMBELAJARAN DISCOVERY LEARNING BERDASARKAN GAYA BELAJAR SISWA

    Directory of Open Access Journals (Sweden)

    Zeni Rofiqoh

    2016-04-01

    Full Text Available Artikel ini ditulis berdasarkan hasil penelitian yang bertujuan untuk memperoleh deskripsi kemampuan pemecahan masalah siswa kelas X berdasarkan gaya belajar siswa dalam pembelajaran discovery learning. Subjek penelitian deskriptif kualitatif ini adalah siswa kelas X MIA 3 MAN 2 Kudus. Pengumpulan data dilakukan melalui angket gaya belajar menurut Kolb, tes kemampuan pemecahan masalah, dan pedoman wawancara. Analisis seluruh data dilakukan dengan langkah-langkah: reduksi data, penyajian data, verifikasi, triangulasi, dan kesimpulan. Hasil penelitian menunjukkan: 1 siswa converger paling banyak jumlahnya di kelas X MIA 3, 2 siswa converger, diverger, accommodator, dan assimilator memahami masalah dengan mengetahui apa yang diketahui dan ditanyakan serta menjelaskan masalah dengan kalimat sendiri. Mereka membuat rencana dengan menyederhanakan masalah, mencari subtujuan, membuat eksperimen dan simulasi, serta mengurutkan informasi. Mereka melaksanakan rencana dengan mengartikan masalah dalam bentuk matematika dan melaksanakan strategi selama penghitungan berlangsung. Siswa converger dan assimilator melihat kembali tanpa mengecek penghitungan yang terlibat, siswa diverger tidak melihat alternatif penyelesaian yang lain dan tidak mengecek penghitungan yang terlibat, siswa accommodator mempertimbangkan bahwa solusi yang diperoleh logis, bertanya kepada diri sendiri apakah pertanyaan sudah terjawab, mengecek penghitungan yang dilakukan, membaca kembali pertanyaan, dan menggunakan alternatif penyelesaian yang lain

  16. Tinjauan Teologis: Allah Menyesal Berdasarkan Perspektif Kitab Kejadian Pasal 6:6-7

    Directory of Open Access Journals (Sweden)

    Yetris Elbaar

    2013-10-01

    Full Text Available Tujuan penelitian ini adalah Pertama, untuk menjelaskan makna Allahmenyesal melalui pemaparan eksposisi nas di dalam kitab Kejadian 6:6-7, supayadapat menghasilkan kerangka pemahaman yang benar tentang Allah sebagaiPencipta. Kedua, untuk mendapatkan pemahaman yang benar mengenai Allahmenyesal berdasarkan penjelasan implikasi teologis. Ketiga, supaya orang percayadapat mengerti makna mengenai Allah menyesal dalam kitab Kejadian 6:6-7 yangdijabarkan dalam implikasi praktis.Penulisan karya ilmiah ini menggunakan metodologi eksposisi, denganmenggunakan prinsip-prinsip hermeneutika, yaitu dengan kajian eksegesis Alkitabmengenai Allah menyesal berdasarkan Kejadian 6:6-7 dan melalui penelitiankualitatif, yaitu dengan penggunaan buku-buku di perpustakaan yang berkaitandengan konsep judul karya ilmiah.Kesimpulan karya ilmiah ini adalah: Pertama, Allah yang menyesal adalahAllah yang konsisten terhadap sifat dan karakter-Nya. Kedua, Allah yang menyesaladalah Allah yang harus dipahami melalui konteks firman Tuhan. Berdasarkankonteks Kejadian 6:6-7 Allah menyesal adalah Allah yang menyatakan pengakuan-Nya sebagai pencipta (6:6. Dan Mengungkapkan keputusan Allah (6:7. Ketiga,Allah menyesal adalah Allah yang tidak dapat menyangkal diri-Nya sebagai Allahyang ingin menyatakan bahwa Ia sangat berduka, tetapi harus menyatakankeadilannya sebagai Allah dan harus menghukum kejahatan manusia. Keempat,Allah menyesal adalah Allah yang menghendaki supaya manusia hidup dalamperaturan dan hukum-hukum yang Allah tetapkan dalam kehidupannya.

  17. Pengembangan Ekonomi Lokal di Kawasan Perikanan Berdasarkan Preferensi Masyarakat Nelayan Nambangan Kedung Cowek

    Directory of Open Access Journals (Sweden)

    Rheza Firmantyo Pramono

    2013-09-01

    Full Text Available Wilayah nambangan memiliki prosentase 40% sebagai nelayan menggantungkan dengan kondisi pesisir, mengindikasikan bahwa wilayah nambangan memiliki potensi pesisir yang cukup besar. Namun masih mempunyai beberapa keterbatasan yang perlu diperhatikan seperti SDA, SDM, Pendanaan, Prasarana Sarana, Kelembagaan, dan teknologi di kawasan pesisir Nambangan Kedung Cowek. Tujuan penelitian ini untuk merumuskan arahan pengembangan ekonomi lokal wilayah nambangan berdasarkan preferensi masyarakat. Metode yang digunakan dalam penelitian ini menggunakan metode deskriptif kualitatif. Analisis pertama penentuan faktor kurang berkembangnya ekonomi lokal menggunakan teknik delphi, selanjutnya menentukan preferensi masyarakat dan faktor prioritas menggunakan teknik servqual. Melalui metode servqual ditemukan banyak faktor prioritas yang harus dikembangkan yaitu SDA, SDM , Pendanaan, Prasarana Sarana, Kelembagaan, Teknologi, dan Pemasaran. Selanjutnya menentukan arahan pengembangan ekonomi lokal beradasakan faktor prioritas menggunakan teknik triangulasi berdasarkan tujuh faktor priortas dengan menggabungkan studi literatur, studi kasus wilayah lain dan temuan fakta emipiri di lapangan. Hasil akhir berupa peningkatan prasarana sarana berupa penataan lingkungan sempadan pantai, pengelolahan limbah, peningkatan sumberdaya manusia berupa pelatihan mutu pendidikan dan wawasan ekonomi. Selanjutnya peningkatan upaya pemasaran dengan membangun mina store dan peningkatan fasilitas alat tangkap.

  18. PERANCANGAN KENDALI NAVIGASI ROBOT TANK SECARA NIRKABEL BERBASIS SENSOR ACCELEROMETER BERDASARKAN GERAKAN TANGAN

    Directory of Open Access Journals (Sweden)

    Muhamad Yusvin Mustar

    2018-04-01

    Full Text Available Sebuah sistem kendali berbeda pada navigasi robot tank diperkenalkan pada penelitian ini. Umumnya sistem kendali navigasi robot tank dikendalikan meggunakan remot kontrol atau joystick dan beberapa perangkat pengontrolan robot lainya. Pengontrolan navigasi robot tank bertujuan untuk dapat mengendalikan pergerakan robot agar dapat berjalan maju, mundur, berbelok ke kiri dan ke kanan. Pada penelitian ini, pengontrolan navigasi robot dilakukan berdasarkan gerakan tangan manusia. Sebuah sarung tangan yang dilengkapi sensor accelerometer ADXL335 didesain untuk dapat mendeteksi setiap bentuk gerakan tangan. Pendeteksian gerakan tangan didasarkan pada pembacaan orientasi axis X dan Y acceleometer. Gerakan tangan ini kemudian diinput pada mikrokontroler Arduino Nano dan ditransmisikan melalui nRF24L01 2.4GHz. Hasil pendeteksian gerak kemudian diterima dan diolah pada mikrokontroler Arduino Mega yang terdapat pada robot tank. Selanjutnya, hasil pendeteksian gerakan tangan ini dipetakan dalam beberapa bagian pengontrolan, agar sesuai dengan pola pengontrolan navigasi robot tank. Hasil penelitian ini menunjukan bahwa sebuah sistem pengontrolan navigasi robot tank berdasarkan gerakan tangan dapat diimplementasikan dan diaplikasikan secara riil, sehingga dapat memberikan pengalaman baru dalam berinteraksi dengan robot. Kata kunci: navigasi, robot tank, deteksi gerakan, accelerometer ADXL335, arduino, nRF24L01.

  19. Pembuatan Perangkat Audit Berbasis Risiko Berdasarkan COBIT 5 dan Service Desk Standard pada Service Desk

    Directory of Open Access Journals (Sweden)

    Sarah Putri Ramadhani

    2017-03-01

    Full Text Available SubDirektorat Layanan Teknologi dan Sistem Informasi pada Direktorat Pengembangan Teknologi dan Sistem Informasi (DPTSI sebagai penyedia layanan TI di lingkungan Institut Teknologi Sepuluh Nopember (ITS tidak sedikit mengalami gangguan atau insiden yang mengakibatkan menurunnya kualitas pelayanan yang diberikan. Oleh karena itu terdapat unit service desk yang bertugas menangani berbagai macam keluhan insiden dan memenuhi permintaan layanan TI. Namun DPTSI belum pernah mengadakan pengendalian internal terhadap prosesnya. Untuk memastikan pengelolaan telah diterapkan dalam kontrolnya maka perlu sebuah metode yaitu audit internal. Salah satu hal yang perlu disiapkan dalam melaksanakan audit adalah perangkat audit karena menyediakan serangkaian instruksi dari proses yang harus dilakukan service desk sehingga membantu seorang auditor dalam menjalankan audit sesuai dengan tujuan dan memastikan seluruh proses telah dilakukan. Berdasarkan permasalahan tersebut, penelitian ini mengembangkan perangkat audit pada service desk DPTSI yang dibuat berdasarkan control objective pada Service Desk Standard yang dipetakan dengan proses pada best practice COBIT 5 domain DSS02. Ruang lingkup perangkat audit juga ditetapkan melalui control objective yang dipetakan dengan risiko TI pada service desk yang dianalisis menggunakan pendekatan best practice COBIT 5 for Risk APO12 Manage Risk. Hasil dari penelitian ini adalah sebuah dokumen perangkat audit beserta panduan penggunaannya, yang nantinya diharapkan dapat membantu DPTSI untuk melakukan audit pada service desk..

  20. A Shld1-controlled POT1a provides support for repression of ATR signaling at telomeres through RPA exclusion.

    Science.gov (United States)

    Gong, Yi; de Lange, Titia

    2010-11-12

    We previously proposed that POT1 prevents ATR signaling at telomeres by excluding RPA from the single-stranded TTAGGG repeats. Here, we use a Shld1-stabilized degron-POT1a fusion (DD-POT1a) to study the telomeric ATR kinase response. In the absence of Shld1, DD-POT1a degradation resulted in rapid and reversible activation of the ATR pathway in G1 and S/G2. ATR signaling was abrogated by shRNAs to ATR and TopBP1, but shRNAs to the ATM kinase or DNA-PKcs did not affect the telomere damage response. Importantly, ATR signaling in G1 and S/G2 was reduced by shRNAs to RPA. In S/G2, RPA was readily detectable at dysfunctional telomeres, and both POT1a and POT1b were required to exclude RPA and prevent ATR activation. In G1, the accumulation of RPA at dysfunctional telomeres was strikingly less, and POT1a was sufficient to repress ATR signaling. These results support an RPA exclusion model for the repression of ATR signaling at telomeres. Copyright © 2010 Elsevier Inc. All rights reserved.

  1. Advanced Test Reactor (ATR) Facility 10CFR830 Safety Basis Related to Facility Experiments

    International Nuclear Information System (INIS)

    Tomberlin, T.A.

    2002-01-01

    The Idaho National Engineering and Environmental Laboratory (INEEL) Advanced Test Reactor (ATR), a DOE Category A reactor, was designed to provide an irradiation test environment for conducting a variety of experiments. The ATR Safety Analysis Report, determined by DOE to meet the requirements of 10 CFR 830, Subpart B, provides versatility in types of experiments that may be conducted. This paper addresses two general types of experiments in the ATR facility and how safety analyses for experiments are related to the ATR safety basis. One type of experiment is more routine and generally represents greater risks; therefore this type of experiment is addressed with more detail in the safety basis. This allows individual safety analyses for these experiments to be more routine and repetitive. The second type of experiment is less defined and is permitted under more general controls. Therefore, individual safety analyses for the second type of experiment tend to be more unique from experiment to experiment. Experiments are also discussed relative to ''major modifications'' and DOE-STD-1027-92. Application of the USQ process to ATR experiments is also discussed

  2. In-situ ATR-FTIR for characterization of thin biorelated polymer films

    International Nuclear Information System (INIS)

    Müller, M.; Torger, B.; Bittrich, E.; Kaul, E.; Ionov, L.; Uhlmann, P.; Stamm, M.

    2014-01-01

    We present and review in-situ-attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic data from thin biorelated polymer films useful for the modification and functionalization of polymer and inorganic materials and discuss their applications related to life sciences. A special ATR mirror attachment operated by the single-beam-sample-reference (SBSR) concept and housing a homebuilt thermostatable flow cell was used, which allows for appropriate background compensation and signal to noise ratio. ATR-FTIR data on the reactive deposition of dopamine on inorganic model surfaces are shown. Information on the structure and deposition pathway for such bioinspired melanin-like films is provided. ATR-FTIR data on thermosensitive polymer brushes of poly(N-isopropylacrylamide) (PNIPAAM) is then presented. The thermotropic hydration and hydrogen bonding behavior of PNIPAAM brush films is described. Finally, ATR-FTIR data on biorelated polyelectrolyte multilayers (PEM) are given together with details on PEM growth and detection. Applications of these latter films for biopassivation/activation and local drug delivery are addressed

  3. PENERAPAN DATAMINING PADA POPULASI DAGING AYAM RAS PEDAGING DI INDONESIA BERDASARKAN PROVINSI MENGGUNAKAN K-MEANS CLUSTERING

    Directory of Open Access Journals (Sweden)

    Mhd Gading Sadewo

    2017-09-01

    Full Text Available Ayam bukanlah makanan yang asing bagi penduduk Indonesia. Makanan tersebut sangat mudah dijumpai dalam kehidupan masyarakat sehari-hari. Namun tingkat konsumsi daging ayam di Indonesia masih tergolong rendah dibandingkan dengan Negara tetangga. Penelitian ini membahas tentang Penerapan Datamining Pada Populasi Daging Ayam Ras Pedaging di Indonesia Berdasarkan Provinsi Menggunakan K-Means Clustering. Sumber data penelitian ini dikumpulkan berdasarkan dokumen-dokumen keterangan populasi daging ayam yang dihasilkan oleh Badan Pusat Statistik Nasional. Data yang digunakan dalam penelitian ini adalah data dari tahun 2009-2016 yang terdiri dari 34 provinsi. Variable yang digunakan (1 jumlah populasi dari tahun 2009-2016. Data akan diolah dengan melakukan clushtering dalam 3 clushter yaitu clusther tingkat populasi tinggi, clusther tingkat populasi sedang dan rendah. Centroid data untuk cluster tingkat populasi tinggi 4711403141, Centroid data untuk cluster tingkat populasi sedang 304240647, dan Centroid data untuk cluster tingkat populasi rendah 554200. Sehingga diperoleh penilaian berdasarkan indeks populasi daging ayam dengan 1 provinsi tingkat populasi tinggi yaitu Jawa Barat, 6 provinsi tingkat populasi sedang yaitu Sumatera Utara, Jawa Tengah, Jawa Timur, Banten, Kalimantan Selatan dan Kalimantan Timur, dan 27 provinsi lainnya termasuk tingkat populasi rendah. Hal ini dapat menjadi masukan kepada pemerintah, provinsi yang menjadi perhatian lebih pada populasi daging ayam berdasarkan cluster yang telah dilakukan

  4. Seeded growth fabrication of Cu-on-Si electrodes for in situ ATR-SEIRAS applications

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Hui-Feng [Shanghai Key Laboratory for Molecular Catalysis and Innovative Materials and Department of Chemistry, Fudan University, Shanghai 200433 (China); Yan, Yan-Gang [Shanghai Key Laboratory for Molecular Catalysis and Innovative Materials and Department of Chemistry, Fudan University, Shanghai 200433 (China); Huo, Sheng-Juan [Shanghai Key Laboratory for Molecular Catalysis and Innovative Materials and Department of Chemistry, Fudan University, Shanghai 200433 (China); Cai, Wen-Bin [Shanghai Key Laboratory for Molecular Catalysis and Innovative Materials and Department of Chemistry, Fudan University, Shanghai 200433 (China); Department of Enviromental Engineering, Shanghai University of Electric Power, Shanghai 200090 (China); E-mail: wbcai@fudan.edu.cn; Xu, Qun-Jie [Department of Enviromental Engineering, Shanghai University of Electric Power, Shanghai 200090 (China); Osawa, Masatoshi [Catalysis Research Center, Hokkaido University, Sapporo 001-0021 (Japan)

    2007-05-25

    A seeded-growth approach has been developed to fabricate a Cu nanoparticle film (simplified hereafter with nanofilm) on Si for electrochemical ATR surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS). The approach comprises an initial activation of the reflecting plane of hemicylindrical Si prism by introducing a Cu seed layer in a CuSO{sub 4}-HF solution and the subsequent electroless deposition of the Cu nanofilms from an electroless Cu plating bath. The as-deposited Cu nanofilm exhibited strong SEIRA effect for the CO probe and interfacial free H{sub 2}O. ATR-SEIRAS was also applied to characterize the adsorbed geometries of pyridine at the Cu/electrolyte interface. Only vibrational bands assignable to the A {sub 1} symmetry modes were detected in the entire potential window investigated, suggestive of an end-on adsorption via the ring N-atom on a Cu electrode.

  5. Measured Thermal and Fast Neutron Fluence Rates for ATF-1 Holders During ATR Cycle 157D

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Larry Don [Idaho National Lab. (INL), Idaho Falls, ID (United States); Miller, David Torbet [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2016-03-01

    This report contains the thermal (2200 m/s) and fast (E>1MeV) neutron fluence rate data for the ATF-1 holders located in core for ATR Cycle 157D which were measured by the Radiation Measurements Laboratory (RML) as requested by the Power Reactor Programs (ATR Experiments) Radiation Measurements Work Order. This report contains measurements of the fluence rates corresponding to the particular elevations relative to the 80-ft. core elevation. The data in this report consist of (1) a table of the ATR power history and distribution, (2) a hard copy listing of all thermal and fast neutron fluence rates, and (3) plots of both the thermal and fast neutron fluence rates. The fluence rates reported are for the average power levels given in the table of power history and distribution.

  6. [Relationship between PMI and ATR-FTIR Spectral Changes in Swine Costal Cartilages and Ribs].

    Science.gov (United States)

    Yao, Yao; Wang, Qi; Jing, Xiao-li; Li, Bing; Zhang, Yin-ming; Wang, Zhi-jun; Li, Cheng-zhi; Lin, Han-cheng; Zhang, Ji; Huang, Ping; Wang, Zhen-yuan

    2016-02-01

    To analyze postmortem chemical changes in Landrace costal cartilages and ribs using attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, and to provide a novel technique for estimation of postmortem interval (PMI). The swines were sacrificed by hemorrhage and their costal cartilages and ribs were kept in 20 degrees C. The chemical analysis of the costal cartilages and ribs were performed using ATR-FTIR every 72 h. The correlation between the certain spectral parameters and PMI was also analyzed. The time-dependent changes of costal cartilages were more significant than ribs. There were no obvious changes for the main absorbance bands position, and some absorbance band ratios showed time-dependent changes and significant correlations with the PMI. ATR-FTIR has the ability to analyze postmortem chemical changes of the swine costal cartilages and ribs, and it can be a new method to estimate PMI based on spectroscopy.

  7. Volvulus and bowel obstruction in ATR-X syndrome-clinical report and review of literature.

    Science.gov (United States)

    Horesh, Nir; Pery, Ron; Amiel, Imri; Shwaartz, Chaya; Speter, Chen; Guranda, Larisa; Gutman, Mordechai; Hoffman, Aviad

    2015-11-01

    Alpha thalassemia-mental retardation, X-linked (ATR-X) syndrome is a rare genetic disorder with a variety of clinical manifestations. Gastrointestinal symptoms described in this syndrome include difficulties in feeding, regurgitation and vomiting which may lead to aspiration pneumonia, abdominal pain, distention, and constipation. We present a 19-year-old male diagnosed with ATR-X syndrome, who suffered from recurrent colonic volvulus that ultimately led to bowel necrosis with severe septic shock requiring emergent surgical intervention. During 1 year, the patient was readmitted four times due to poor oral intake, dehydration and abdominal distention. Investigation revealed partial small bowel volvulus which resolved with non-operative treatment. Small and large bowel volvulus are uncommon and life-threatening gastrointestinal manifestations of ATR-X patients, which may contribute to the common phenomenon of prolonged food refusal in these patients. © 2015 Wiley Periodicals, Inc.

  8. Seeded growth fabrication of Cu-on-Si electrodes for in situ ATR-SEIRAS applications

    International Nuclear Information System (INIS)

    Wang, Hui-Feng; Yan, Yan-Gang; Huo, Sheng-Juan; Cai, Wen-Bin; Xu, Qun-Jie; Osawa, Masatoshi

    2007-01-01

    A seeded-growth approach has been developed to fabricate a Cu nanoparticle film (simplified hereafter with nanofilm) on Si for electrochemical ATR surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS). The approach comprises an initial activation of the reflecting plane of hemicylindrical Si prism by introducing a Cu seed layer in a CuSO 4 -HF solution and the subsequent electroless deposition of the Cu nanofilms from an electroless Cu plating bath. The as-deposited Cu nanofilm exhibited strong SEIRA effect for the CO probe and interfacial free H 2 O. ATR-SEIRAS was also applied to characterize the adsorbed geometries of pyridine at the Cu/electrolyte interface. Only vibrational bands assignable to the A 1 symmetry modes were detected in the entire potential window investigated, suggestive of an end-on adsorption via the ring N-atom on a Cu electrode

  9. Analysis of cosmetic residues on a single human hair by ATR FT-IR microspectroscopy

    Science.gov (United States)

    Pienpinijtham, Prompong; Thammacharoen, Chuchaat; Naranitad, Suwimol; Ekgasit, Sanong

    2018-05-01

    In this work, ATR FT-IR spectra of single human hair and cosmetic residues on hair surface are successfully collected using a homemade dome-shaped Ge μIRE accessary installed on an infrared microscope. By collecting ATR spectra of hairs from the same person, the spectral patterns are identical and superimposed while different spectral features are observed from ATR spectra of hairs collected from different persons. The spectral differences depend on individual hair characteristics, chemical treatments, and cosmetics on hair surface. The "Contact-and-Collect" technique that transfers remarkable materials on the hair surface to the tip of the Ge μIRE enables an identification of cosmetics on a single hair. Moreover, the differences between un-split and split hairs are also studied in this report. These highly specific spectral features can be employed for unique identification or for differentiation of hairs based on the molecular structures of hairs and cosmetics on hairs.

  10. Arahan Pengembangan Kawasan Wisata Pantai Nepa Berdasarkan Preferensi Pengunjung Kecamatan Banyuates Kabupaten Sampang

    Directory of Open Access Journals (Sweden)

    Tadaki Santoso Hasegawa

    2017-03-01

    Full Text Available Kecamatan Banyuates, Kabupaten Sampang, memiliki kawasan wisata pantai Nepa, yang terdiri dari 6 potensi wisata di 3 desa, yakni wisata alam pantai Nepa, wisata alam hutan kera Nepa, makam petilasan Raden Segoro, wisata arung laut, wisata budaya Rokat Tase’, dan wisata buatan waduk Nipah, di Desa Batioh, Desa Nepa, dan Desa Montor. Penelitian deskriptif ini menggunakan analisis deskriptif, yang digunakan untuk mencapai sasaran pertama analisis potensi wisata, sasaran kedua analisis preferensi pengunjung, hingga sasaran terakhir merumuskan arahan pengembangan kawasan wisata pantai Nepa berdasarkan preferensi pengunjung. Rumusan arahan pengembangan kawasan tersebut menghasilkan arahan pengembangan berupa penyediaan, perbaikan, pemeliharaan, dan peningkatan akses prasarana dan sarana pariwisata, peningkatan kesadaran terhadap kelestarian lingkungan dan sikap masyarakat terhadap pengunjung dengan nilai-nilai sapta pesona, penambahan jenis atraksi wisata, penyediaan akomodasi, peningkatan partisipasi masyarakat, dan promosi kawasan, untuk setiap potensi wisata.

  11. Deteksi Kematangan Buah Pisang Berdasarkan Fitur Warna Citra Kulit Pisang Menggunakan Metode Transformasi Ruang Warna HIS

    Directory of Open Access Journals (Sweden)

    Indarto

    2017-05-01

    Full Text Available Pengolahan citra mempunyai peranan penting di berbagai bidang. Aplikasi pengolahan citra berkaitan dengan pemrosesan citra berkaitan dengan transformasi warna. Dalam paper ini dijelaskan mengenai metode transformasi warna HSI untuk deteksi pisang dan mengklasifikasikan dalam fase kematangan. Data dalam penelitian ini menggunakan citra pisang ambon yang diambil dengan kamera yang nantinya akan diambil sebagian (cropping pada kulit, yang kemudian akan diekstrak ciri warnanya, dan dihitung tingkat kadar warna dari R (red, G (green, dan B (blue dan diubah ke HSI. Berdasarkan input pelatihan deteksi jenis warna kulit pisang ambon diperoleh dari pengolahan citra dengan metode transformasi warna HSI. Dari hasil penelitian 20 sampel buah dimana 10 buah pisang ambon mentah dan 10 buah pisang ambon matang dengan dihitung nilai rata-rata maksimal dan minimal H dan S diperoleh akurasi kesesuaian sebesar 85%.

  12. SAR image dataset of military ground targets with multiple poses for ATR

    Science.gov (United States)

    Belloni, Carole; Balleri, Alessio; Aouf, Nabil; Merlet, Thomas; Le Caillec, Jean-Marc

    2017-10-01

    Automatic Target Recognition (ATR) is the task of automatically detecting and classifying targets. Recognition using Synthetic Aperture Radar (SAR) images is interesting because SAR images can be acquired at night and under any weather conditions, whereas optical sensors operating in the visible band do not have this capability. Existing SAR ATR algorithms have mostly been evaluated using the MSTAR dataset.1 The problem with the MSTAR is that some of the proposed ATR methods have shown good classification performance even when targets were hidden,2 suggesting the presence of a bias in the dataset. Evaluations of SAR ATR techniques are currently challenging due to the lack of publicly available data in the SAR domain. In this paper, we present a high resolution SAR dataset consisting of images of a set of ground military target models taken at various aspect angles, The dataset can be used for a fair evaluation and comparison of SAR ATR algorithms. We applied the Inverse Synthetic Aperture Radar (ISAR) technique to echoes from targets rotating on a turntable and illuminated with a stepped frequency waveform. The targets in the database consist of four variants of two 1.7m-long models of T-64 and T-72 tanks. The gun, the turret position and the depression angle are varied to form 26 different sequences of images. The emitted signal spanned the frequency range from 13 GHz to 18 GHz to achieve a bandwidth of 5 GHz sampled with 4001 frequency points. The resolution obtained with respect to the size of the model targets is comparable to typical values obtained using SAR airborne systems. Single polarized images (Horizontal-Horizontal) are generated using the backprojection algorithm.3 A total of 1480 images are produced using a 20° integration angle. The images in the dataset are organized in a suggested training and testing set to facilitate a standard evaluation of SAR ATR algorithms.

  13. ATR and transmission analysis of pigments by means of far infrared spectroscopy.

    Science.gov (United States)

    Kendix, Elsebeth L; Prati, Silvia; Joseph, Edith; Sciutto, Giorgia; Mazzeo, Rocco

    2009-06-01

    In the field of FTIR spectroscopy, the far infrared (FIR) spectral region has been so far less investigated than the mid-infrared (MIR), even though it presents great advantages in the characterization of those inorganic compounds, which are inactive in the MIR, such as some art pigments, corrosion products, etc. Furthermore, FIR spectroscopy is complementary to Raman spectroscopy if the fluorescence effects caused by the latter analytical technique are considered. In this paper, ATR in the FIR region is proposed as an alternative method to transmission for the analyses of pigments. This methodology was selected in order to reduce the sample amount needed for analysis, which is a must when examining cultural heritage materials. A selection of pigments have been analyzed in both ATR and transmission mode, and the resulting spectra were compared with each other. To better perform this comparison, an evaluation of the possible effect induced by the thermal treatment needed for the preparation of the polyethylene pellets on the transmission spectra of the samples has been carried out. Therefore, pigments have been analyzed in ATR mode before and after heating them at the same temperature employed for the polyethylene pellet preparation. The results showed that while the heating treatment causes only small changes in the intensity of some bands, the ATR spectra were characterized by differences in both intensity and band shifts towards lower frequencies if compared with those recorded in transmission mode. All pigments' transmission and ATR spectra are presented and discussed, and the ATR method was validated on a real case study.

  14. 10 CFR 830 Major Modification Determination for the ATR Diesel Bus (E-3) and Switchgear Replacement

    International Nuclear Information System (INIS)

    Duckwitz, Noel

    2011-01-01

    Near term replacement of aging and obsolescent original ATR equipment has become important to ensure ATR capability in support of NE's long term national missions. To that end, a mission needs statement has been prepared for a non-major system acquisition which is comprised of three interdependent subprojects. The first project, subject of this determination, will replace the existent diesel-electrical bus (E-3) and associated switchgear. More specifically, INL proposes transitioning ATR to 100% commercial power with appropriate emergency backup to include: (1) Provide commercial power as the normal source of power to the ATR loads currently supplied by diesel-electric power. (2) Provide backup power to the critical ATR loads in the event of a loss of commercial power. (3) Replace obsolescent critical ATR power distribution equipment, e.g., switchgear, transformers, motor control centers, distribution panels. Completion of this and two other age-related projects (primary coolant pump and motor replacement and emergency firewater injection system replacement) will resolve major age related operational issues plus make a significant contribution in sustaining the ATR safety and reliability profile. The major modification criteria evaluation of the project pre-conceptual design identified several issues make the project a major modification: (1) Evaluation Criteria No.2 (Footprint change). The addition of a new PC-4 structure to the ATR Facility to house safety-related SSCs requires careful attention to maintaining adherence to applicable engineering and nuclear safety design criteria (e.g., structural qualification, fire suppression) to ensure no adverse impacts to the safety-related functions of the housed equipment. (2) Evaluation Criteria No.3 (Change of existing process). The change to the strategy for providing continuous reliable power to the safety-related emergency coolant pumps requires careful attention and analysis to ensure it meets a project primary

  15. ATM/ATR-mediated phosphorylation of PALB2 promotes RAD51 function

    DEFF Research Database (Denmark)

    Ahlskog, Johanna K; Larsen, Brian D; Achanta, Kavya

    2016-01-01

    DNA damage activates the ATM and ATR kinases that coordinate checkpoint and DNA repair pathways. An essential step in homology-directed repair (HDR) of DNA breaks is the formation of RAD51 nucleofilaments mediated by PALB2-BRCA2; however, roles of ATM and ATR in this critical step of HDR are poor...... function, as the PALB2-dependent checkpoint response is normal in cells expressing the phospho-deficient PALB2 mutant. Collectively, our findings highlight a critical importance of PALB2 phosphorylation as a novel regulatory step in genome maintenance after genotoxic stress....

  16. L'Atrésie colique:À propos de deux cas

    African Journals Online (AJOL)

    ... dans un tableau d'occlusion néonatale haute. L'atrésie colique était de découverte fortuite en per-opératoire, associée à une atrésie grêlique. Le patient a bénéficié d'une double stomie, mais les suites opératoires ont été marquées par le décès dans un tableau de choc septique. A la lumière des ces deux observations ...

  17. In vitro analysis of the role of replication protein A (RPA) and RPA phosphorylation in ATR-mediated checkpoint signaling.

    Science.gov (United States)

    Lindsey-Boltz, Laura A; Reardon, Joyce T; Wold, Marc S; Sancar, Aziz

    2012-10-19

    Replication protein A (RPA) plays essential roles in DNA metabolism, including replication, checkpoint, and repair. Recently, we described an in vitro system in which the phosphorylation of human Chk1 kinase by ATR (ataxia telangiectasia mutated and Rad3-related) is dependent on RPA bound to single-stranded DNA. Here, we report that phosphorylation of other ATR targets, p53 and Rad17, has the same requirements and that RPA is also phosphorylated in this system. At high p53 or Rad17 concentrations, RPA phosphorylation is inhibited and, in this system, RPA with phosphomimetic mutations cannot support ATR kinase function, whereas a non-phosphorylatable RPA mutant exhibits full activity. Phosphorylation of these ATR substrates depends on the recruitment of ATR and the substrates by RPA to the RPA-ssDNA complex. Finally, mutant RPAs lacking checkpoint function exhibit essentially normal activity in nucleotide excision repair, revealing RPA separation of function for checkpoint and excision repair.

  18. In Vitro Analysis of the Role of Replication Protein A (RPA) and RPA Phosphorylation in ATR-mediated Checkpoint Signaling*

    Science.gov (United States)

    Lindsey-Boltz, Laura A.; Reardon, Joyce T.; Wold, Marc S.; Sancar, Aziz

    2012-01-01

    Replication protein A (RPA) plays essential roles in DNA metabolism, including replication, checkpoint, and repair. Recently, we described an in vitro system in which the phosphorylation of human Chk1 kinase by ATR (ataxia telangiectasia mutated and Rad3-related) is dependent on RPA bound to single-stranded DNA. Here, we report that phosphorylation of other ATR targets, p53 and Rad17, has the same requirements and that RPA is also phosphorylated in this system. At high p53 or Rad17 concentrations, RPA phosphorylation is inhibited and, in this system, RPA with phosphomimetic mutations cannot support ATR kinase function, whereas a non-phosphorylatable RPA mutant exhibits full activity. Phosphorylation of these ATR substrates depends on the recruitment of ATR and the substrates by RPA to the RPA-ssDNA complex. Finally, mutant RPAs lacking checkpoint function exhibit essentially normal activity in nucleotide excision repair, revealing RPA separation of function for checkpoint and excision repair. PMID:22948311

  19. Measured thermal and fast neutron fluence rates for ATF-1 holders during ATR cycle 160A

    International Nuclear Information System (INIS)

    Walker, B. J.; Miller, D. T.

    2017-01-01

    This report contains the thermal (2200 m/s) and fast (E>1MeV) neutron fluence rate data for the ATF-1 holders located in core for ATR Cycle 160A which were measured by the Radiation Measurements Laboratory (RML).

  20. Increased Rrm2 gene dosage reduces fragile site breakage and prolongs survival of ATR mutant mice

    DEFF Research Database (Denmark)

    Lopez-Contreras, Andres J; Specks, Julia; Barlow, Jacqueline H

    2015-01-01

    In Saccharomyces cerevisiae, absence of the checkpoint kinase Mec1 (ATR) is viable upon mutations that increase the activity of the ribonucleotide reductase (RNR) complex. Whether this pathway is conserved in mammals remains unknown. Here we show that cells from mice carrying extra alleles of the...

  1. ATR Mediates a Checkpoint at the Nuclear Envelope in Response to Mechanical Stress

    Czech Academy of Sciences Publication Activity Database

    Kumar, A.; Mazzanti, M.; Mistrik, M.; Košař, Martin; Beznoussenko, G.V.; Mironov, A. A.; Garrè, M.; Parazolli, D.; Shivashankar, G. V.; Scita, G.; Bartek, Jiří; Foiani, M.

    2014-01-01

    Roč. 158, č. 3 (2014), s. 633-646 ISSN 0092-8674 Grant - others:Marie Curie Intra-European(IT) 274093 Institutional support: RVO:68378050 Keywords : ATR * Mechanical Stress * cell cycle Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 32.242, year: 2014

  2. Measured thermal and fast neutron fluence rates for ATF-1 holders during ATR cycle 160A

    Energy Technology Data Exchange (ETDEWEB)

    Walker, B. J. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Miller, D. T. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2017-06-06

    This report contains the thermal (2200 m/s) and fast (E>1MeV) neutron fluence rate data for the ATF-1 holders located in core for ATR Cycle 160A which were measured by the Radiation Measurements Laboratory (RML).

  3. Multivariate analysis of ATR-FTIR spectra for assessment of oil shale organic geochemical properties

    Science.gov (United States)

    Washburn, Kathryn E.; Birdwell, Justin E.

    2013-01-01

    In this study, attenuated total reflectance (ATR) Fourier transform infrared spectroscopy (FTIR) was coupled with partial least squares regression (PLSR) analysis to relate spectral data to parameters from total organic carbon (TOC) analysis and programmed pyrolysis to assess the feasibility of developing predictive models to estimate important organic geochemical parameters. The advantage of ATR-FTIR over traditional analytical methods is that source rocks can be analyzed in the laboratory or field in seconds, facilitating more rapid and thorough screening than would be possible using other tools. ATR-FTIR spectra, TOC concentrations and Rock–Eval parameters were measured for a set of oil shales from deposits around the world and several pyrolyzed oil shale samples. PLSR models were developed to predict the measured geochemical parameters from infrared spectra. Application of the resulting models to a set of test spectra excluded from the training set generated accurate predictions of TOC and most Rock–Eval parameters. The critical region of the infrared spectrum for assessing S1, S2, Hydrogen Index and TOC consisted of aliphatic organic moieties (2800–3000 cm−1) and the models generated a better correlation with measured values of TOC and S2 than did integrated aliphatic peak areas. The results suggest that combining ATR-FTIR with PLSR is a reliable approach for estimating useful geochemical parameters of oil shales that is faster and requires less sample preparation than current screening methods.

  4. Solid Acid-Catalyzed Cellulose Hydrolysis Monitored by In Situ ATR-IR Spectroscopy

    NARCIS (Netherlands)

    Zakzeski, J.; Grisel, R.J.H.; Smit, A.T.; Weckhuysen, B.M.

    2012-01-01

    The solid acid-catalyzed hydrolysis of cellulose was studied under elevated temperatures and autogenous pressures using in situ ATR-IR spectroscopy. Standards of cellulose and pure reaction products, which include glucose, fructose, hydroxymethylfurfural (HMF), levulinic acid (LA), formic acid, and

  5. Human papillomavirus detection using PCR and ATR-FTIR for cervical cancer screening

    Science.gov (United States)

    Rymsza, Taciana; Ribeiro, Eliane Aline; de Carvalho, Luis Felipe das Chagas e. Silva; Bhattacharjee, Tanmoy; de Azevedo Canevari, Renata

    2018-05-01

    The human papillomavirus (HPV) genital infection is considered one of the most common sexually transmitted diseases worldwide, and has been associated with cervical cancer. The objective of this study was to investigate the efficacy of the diagnostic methods: polymerase chain reaction (PCR) and Fourier transform infrared (FTIR) equipped with an ATR (Attenuated Total Reflectance) unit (Pike Tech) spectroscopy, to diagnose HPV infection in women undergoing gynecological examination. Seventeen patients (41.46%) of the 41 patients analyzed were diagnosed with exophytic/condyloma acuminate lesions by clinical analysis, 29 patients (70.7%) (G1 group) of the 41 patients, showed positive result for HPV cell injury by oncotic colpocitology and 12 patients (29.3%) (G2 group), presented negative result for cellular lesion and absence of clinical HPV lesion. Four samples were obtained per patient, which were submitted oncotic colpocitology analysis (Papanicolau staining, two samples), PCR (one sample) and ATR-FTIR analysis (one sample). L1 gene was amplified by PCR technique with specific GP5+/GP6+ and MY09/MY11 primers. PCR results were uniformly positive for presence of HPV in all analyzed samples. Multivariate analysis of ATR-FTIR spectra suggests no significant biochemical changes between groups and no clustering formed, concurring with results of PCR. This study suggests that PCR and ATR-FTIR are highly sensitive technique for HPV detection.

  6. 30 CFR 75.209 - Automated Temporary Roof Support (ATRS) systems.

    Science.gov (United States)

    2010-07-01

    ... of temporary support shall be used, as specified in the roof control plan, when— (1) Mining... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Automated Temporary Roof Support (ATRS) systems... COAL MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Roof Support § 75.209...

  7. DNA-PKcs, ATM, and ATR Interplay Maintains Genome Integrity during Neurogenesis.

    Science.gov (United States)

    Enriquez-Rios, Vanessa; Dumitrache, Lavinia C; Downing, Susanna M; Li, Yang; Brown, Eric J; Russell, Helen R; McKinnon, Peter J

    2017-01-25

    The DNA damage response (DDR) orchestrates a network of cellular processes that integrates cell-cycle control and DNA repair or apoptosis, which serves to maintain genome stability. DNA-PKcs (the catalytic subunit of the DNA-dependent kinase, encoded by PRKDC), ATM (ataxia telangiectasia, mutated), and ATR (ATM and Rad3-related) are related PI3K-like protein kinases and central regulators of the DDR. Defects in these kinases have been linked to neurodegenerative or neurodevelopmental syndromes. In all cases, the key neuroprotective function of these kinases is uncertain. It also remains unclear how interactions between the three DNA damage-responsive kinases coordinate genome stability, particularly in a physiological context. Here, we used a genetic approach to identify the neural function of DNA-PKcs and the interplay between ATM and ATR during neurogenesis. We found that DNA-PKcs loss in the mouse sensitized neuronal progenitors to apoptosis after ionizing radiation because of excessive DNA damage. DNA-PKcs was also required to prevent endogenous DNA damage accumulation throughout the adult brain. In contrast, ATR coordinated the DDR during neurogenesis to direct apoptosis in cycling neural progenitors, whereas ATM regulated apoptosis in both proliferative and noncycling cells. We also found that ATR controls a DNA damage-induced G 2 /M checkpoint in cortical progenitors, independent of ATM and DNA-PKcs. These nonoverlapping roles were further confirmed via sustained murine embryonic or cortical development after all three kinases were simultaneously inactivated. Thus, our results illustrate how DNA-PKcs, ATM, and ATR have unique and essential roles during the DDR, collectively ensuring comprehensive genome maintenance in the nervous system. The DNA damage response (DDR) is essential for prevention of a broad spectrum of different human neurologic diseases. However, a detailed understanding of the DDR at a physiological level is lacking. In contrast to many in

  8. Protein phosphatase 5 is necessary for ATR-mediated DNA repair

    International Nuclear Information System (INIS)

    Kang, Yoonsung; Cheong, Hyang-Min; Lee, Jung-Hee; Song, Peter I.; Lee, Kwang-Ho; Kim, Sang-Yong; Jun, Jae Yeoul; You, Ho Jin

    2011-01-01

    Research highlights: → Serine/threonine protein phosphatase 5 (PP5) has been shown to participate in ataxia telangiectasia-mutated (ATM)- and ATR (ATM- and Rad3-related)-mediated checkpoint pathways, which plays an important role in the DNA damage response and maintenance of genomic stability. → However, it is not clear exactly how PP5 participates in this process. → Our results indicate that PP5 is more closely related with ATR-mediated pathway than ATM-mediated pathway in DNA damage repair. -- Abstract: Several recent studies have shown that protein phosphatase 5 (PP5) participates in cell cycle arrest after DNA damage, but its roles in DNA repair have not yet been fully characterized. We investigated the roles of PP5 in the repair of ultraviolet (UV)- and neocarzinostatin (NCS)-induced DNA damage. The results of comet assays revealed different repair patterns in UV- and NCS-exposed U2OS-PS cells. PP5 is only essential for Rad3-related (ATR)-mediated DNA repair. Furthermore, the phosphorylation of 53BP1 and BRCA1, important mediators of DNA damage repair, and substrates of ATR and ATM decreased in U2OS-PS cells exposed to UV radiation. In contrast, the cell cycle arrest proteins p53, CHK1, and CHK2 were normally phosphorylated in U2OS and U2OS-PS cells exposed to UV radiation or treated with NCS. In view of these results, we suggest that PP5 plays a crucial role in ATR-mediated repair of UV-induced DNA damage.

  9. VE-821, an ATR inhibitor, causes radiosensitization in human tumor cells irradiated with high LET radiation

    International Nuclear Information System (INIS)

    Fujisawa, Hiroshi; Nakajima, Nakako Izumi; Sunada, Shigeaki; Lee, Younghyun; Hirakawa, Hirokazu; Yajima, Hirohiko; Fujimori, Akira; Uesaka, Mitsuru; Okayasu, Ryuichi

    2015-01-01

    High linear energy transfer (LET) radiation such as carbon ion particles is successfully used for treatment of solid tumors. The reason why high LET radiation accomplishes greater tumor-killing than X-rays is still not completely understood. One factor would be the clustered or complex-type DNA damages. We previously reported that complex DNA double-strand breaks produced by high LET radiation enhanced DNA end resection, and this could lead to higher kinase activity of ATR protein recruited to RPA-coated single-stranded DNA. Although the effect of ATR inhibition on cells exposed to low LET gamma-rays has recently been reported, little is known regarding the effect of ATR inhibitor on cells treated with high LET radiation. The purpose of this study is to investigate the effects of the ATR inhibitor VE-821 in human tumor and normal cells irradiated with high LET carbon ions. HeLa, U2OS, and 1BR-hTERT (normal) cells were pre-treated with 1 μM VE-821 for 1 hour and irradiated with either high LET carbon ions or X-rays. Cell survival, cell cycle distribution, cell growth, and micronuclei formation were evaluated. VE-821 caused abrogation of G2/M checkpoint and forced irradiated cells to divide into daughter cells. We also found that carbon ions caused a higher number of multiple micronuclei than X-rays, leading to decreased cell survival in tumor cells when treated with VE-821, while the survival of irradiated normal cells were not significantly affected by this inhibitor. ATR inhibitor would be an effective tumor radiosensitizer with carbon ion irradiation. The online version of this article (doi:10.1186/s13014-015-0464-y) contains supplementary material, which is available to authorized users

  10. In situ detection of cancerous kidney tissue by means of fiber ATR-FTIR spectroscopy

    Science.gov (United States)

    Sablinskas, Valdas; Velicka, Martynas; Pucetaite, Milda; Urboniene, Vidita; Ceponkus, Justinas; Bandzeviciute, Rimante; Jankevicius, Feliksas; Sakharova, Tatiana; Bibikova, Olga; Steiner, Gerald

    2018-02-01

    The crucial goal of kidney-sparing surgical resection of a malignant tumor is complete removal of the cancerous tissue. The exact border between the cancerous and normal tissues is not always possible to identify by naked eye, therefore, a supplementary intraoperative diagnosis is needed. Unfortunately, intraoperative pathology methods used nowadays are time consuming and of inadequate quality rendering not definitive diagnosis. It has recently been shown that ATR-FTIR spectroscopy can be used for fast discrimination between cancerous and normal kidney tissues by analyzing the collected spectra of the tissue touch imprint smears. Most prominent differences are obtained in the wavenumber region from 950 cm-1 to 1250 cm-1, where the spectral bands due to the molecular vibrations of glycogen arise in the spectra of cancerous tissue smears. Such method of detection of cancerous tissue is limited by requirement to transfer the suspected tissue from the body to the FTIR instrument and stamp it on an ATR crystal of the spectrometer. We propose a spectroscopic tool which exploits the same principle of detection of cancerous cells as mentioned above, but does not require the tissue to be transferred from the body to the spectrometer. The portable spectrometer used in this design is equipped with fiber ATR probe and a sensitive liquid nitrogen cooled MCT detector. The design of the fiber probe allows the ATR tip to be changed easily in order to use only new sterilized tips for each measurement point of the tissue. It also enables sampling multiple areas of the suspected tissue with high lateral resolution which, in turn, increases accuracy with which the marginal regions between normal and cancerous tissues can be identified. Due to the loss of optical signal in the fiber probe the spectra have lower signal-to-noise ratio than in the case of standard ATR sampling setup. However, software for the spectral analysis used with the fiber probe design is still able to distinguish

  11. Sistem Pemilihan Mesin Cuci Berdasarkan Kebutuhan Konsumen Menggunakan Fuzzy Tahani dan Promethee

    Directory of Open Access Journals (Sweden)

    Nur Huzumah

    2018-04-01

    Full Text Available Abstrak Berdasarkan hasil survei terhadap kebutuhan mesin cuci pada tahun 2013 pertumbuhan mesin cuci nasional naik sebesar 28%, Menurut data Euro Monitor tahun 2017, penetrasi mesin cuci di Indonesia selama lima tahun terakhir terus meningkat. Sekitar 74% masyarakat di Kota besar seperti Jakarta telah mengandalkan mesin cuci untuk membantu membersihkan pakaian. Namun pada pembelian mesin cuci, sebagian besar masyarakat masih tertarik dengan harga yang murah tanpa mengetahui spesifikasi detail mengenai mesin cuci yang akan dibeli apakah sesuai kebutuhan atau tidak. Karena kurangnya kesadaran masyarakat, maka dibuat sebuah sistem pendukung keputusan menggunakan metode fuzzy tahani dan promethee untuk meningkatkan kualitas rekomendasi mesin cuci sesuai kebutuhan konsumen. Metode fuzzy tahani digunakan untuk melakukan pengurutan data mesin cuci berdasarkan kriteria yang menghasilkan nilai 0 sampai dengan 1. Setelah itu, metode promethee digunakan untuk mendapatkan bobot mesin cuci dan akan dilakukan pengurutan mesin cuci sesuai bobot yang didapat. Hasil penelitian ini menunjukkan bahwa adanya sistem pendukung keputusan yang dibangun, masyarakat yang ingin membeli mesin cuci dapat memilih dengan tepat, jelas dan objektif. Hasil akurasi yang dihasilkan adalah 90%. Kata Kunci: sistem pendukung keputusan, pemilihan mesin cuci, fuzzy tahani, promethee Abstract Based on results of survey about the needs of washing machines in 2013, the growth of national washing machines increased by 28%. According to Euro Monitor Data of 2017, penetration of washing machines in Indonesia over the last five years continues to increase. About 74 % of the people in big cities like Jakarta have relied on a washing machine to help clean clothes. However on the purchase of a washing machine, most of the people still interested in a cheap price without knowing detail specification of the washing machine which will be purchased whether as needed or not. Due the lack of public

  12. Profil Berpikir Geometri Siswa Tunagrahita Berdasarkan Tingkatan Van Hiele Di SMPLB Negeri Salatiga

    Directory of Open Access Journals (Sweden)

    Tri Nova Hasti Yunianta

    2017-06-01

    Full Text Available Jenis penelitian ini adalah deskriptif kualitatif yang bertujuan untuk mengetahui bagaimana profil berpikir geometri siswa tunagrahita berdasarkan tingkatan berpikir van Hiele. Subjek dalam penelitian ini adalah tiga orang siswa yang terdiri masing-masing satu siswa dari kelas VII, VIII dan IX SMPLB C Negeri Salatiga. Penelitian ini dilakukan melalui wawancara semi terstrukur. Hasil dari penelitian yang dilakukan, ketiga siswa cenderung memiliki kemampuan yang sama dalam berpikir geometri. Ketiga siswa dapat mencapai tingkat 1 (visualisasi berdasarkan tingkat berpikir geometri van Hiele, namun terbatas pada bentuk-bentuk bangun geometri yang dipahami atau dikenalnya. Siswa belum dapat melakukan analisis pada tingkat 2 dan hanya sebatas mengetahui sebagian komponen pada bangun segiempat serta menamainya menggunakan bahasanya sendiri yang tidak baku. Selain itu siswa mengetahui beberapa istilah yang ada pada bangun segiempat, namun tidak mengetahui maksud dari istilah-istilah tersebut. Kendala dan masalah yang dihadapi ketiga siswa yaitu lebih cenderung memperhatikan pada bangun-bangun yang dikenalnya, kurang cermat dan teliti dalam menggambar, kesulitan dalam menjelaskan atau mendeskripsikan secara lisan suatu bangun segiempat dan hanya terbatas pada bangun yang diketahuinya, hal tesebut karena siswa tidak banyak mengenal macam-macam bangun.This research is a qualitative descriptive which aims to determine how the profile geometry students think retarded by van Hiele levels of thinking. Subjects in this study were three students who comprised each of the students of class VII, VIII and IX SMPLB C State Salatiga. This research was conducted through semi-structured interviews. The results of the research conducted, the three students tend to have the same ability to think geometry. All three students can reach level 1 (visualization based on the geometry of the van Hiele levels of thinking, but limited to the forms of geometry understood or known

  13. IDENTIFIKASI JENIS OBAT BERDASARKAN GAMBAR LOGO PADA KEMASAN MENGGUNAKAN METODE NAIVE BAYES

    Directory of Open Access Journals (Sweden)

    Surya Rahayuda

    2016-10-01

    Full Text Available There many types of drugs have been approved by the government and circulating in the community, but many people don’t know. In this study, I want to create an application that can identify the type of drug based on the logo on the packaging. I’m using 4 different types of modern medicine and 3 types of herbal medicine, total there will be as many as 7 different logo that will be used. Pictures will be entered into the application, then detected the edges of the image using the Edge Detection, to get the shape of the logo image, after it is extracted using methods GLCM, extraction will produce output in the form of numbers, the numeric data is then classified using Naïve Bayes classification and will get the results in the form of the type of drug. From the experiments it was found that the resulting level of accuracy is quite high, there are 3 categories of types of drugs that have a high accuracy on Obat Bebas, Obat Bebas Terbatas and Obat Keras. From the results of these trials concluded that the Naïve Bayes method can be used to mengkalsifikasi types of drugs is based on the logo on the packaging of drugs. Keywords: logo, drug, image processing, edge detection, GLCM, naïve bayes Terdapat banyak jenis obat telah disetujui oleh pemerintah dan beredar di masyarakat, namun banyak masyarakat tidak mengetahuinya. Pada penelitian ini saya ingin membuat suatu aplikasi yang dapat mengindentifikasi jenis obat berdasarkan logo pada kemasan. Saya menggunakan 4 jenis obat moderen dan 3 jenis obat herbal, total akan terdapat sebanyak 7 macam logo yang akan digunakan. Gambar akan diinputkan ke dalam aplikasi, kemudian dideteksi tepian gambarnya menggunakan metode Edge Detection, untuk mendapatkan bentuk dari gambar logo, setelah itu diekstraksi menggunakan metode GLCM, hasil ekstraksi akan menghasilkan output berupa angka, data angka ini kemudian diklasifikasikan menggunakan metode Naïve Bayes dan akan mendapatkan hasil klasifikasi berupa jenis

  14. USULAN PERANCANGAN TATA LETAK PENYIMPANAN KOMPONEN BERDASARKAN KRITERIA KOMODITI KOMPONEN (Studi Kasus di PT Triangle Motorindo Semarang

    Directory of Open Access Journals (Sweden)

    Sri Hartini

    2012-02-01

    Full Text Available Kurang lancarnya suplai komponen ke bagian produksi dapat menyebabkan menurunnya produktivitas dari bagian produksi. Untuk itu perlu dilakukan perancangan tata letak penyimpanan komponen yang lebih baik sehingga dapat mengurangi waktu mencari komponen, mengurangi jarak perjalanan operator dalam pengambilan dan pengiriman komponen, dan meningkatkan pemanfaatan kapasitas gudang.                 PT. Triangle Motorindo merupakan perusahaan yang bergerak dalam usaha perakitan sepeda motor. Secara garis besar, perusahaan dapat dibagi menjadi dua bagian yaitu bagian gudang dan bagian produksi dimana bagian gudang menjadi pendukung dari bagian produksi. Bagian gudang terdiri dari gudang sortir, gudang rangka, dan gudang mesin. Dalam mengalokasikan komponen yang disimpan, perusahaan menggunakan kebijakan penyimpanan random/acak yang berakibat tidak standarnya waktu yang dibutuhkan untuk mencari komponen dan bertambahnya waktu perjalanan operator dalam menyuplai komponen. Penelitian ini memberikan alternatif perancangan tata letak penyimpanan komponen yang dilakukan dengan memperhatikan komponen itu sendiri, dalam hal ini disebut faktor komoditi yang terdiri atas popularity, similarity, characteristic, dan size. Selain berdasarkan komponen yang disimpan, perancangan tata letak penyimpanan ini juga memperhatikan kondisi ruangan yang tersedia. Hasil pengolahan data dan analisis yang dilakukan menunjukkan bahwa tata letak penyimpanan komponen yang terbaik adalah berdasarkan kriteria process similarity. Tata letak penyimpanan komponen berdasarkan kriteria process similarity ini merupakan tata letak penyimpanan komponen terbaik. Kata Kunci : komponen, faktor komoditi, lost time, waktu perjalanan

  15. KEEFEKTIFAN MODEL SHOW NOT TELL DAN MIND MAP PADA PEMBELAJARAN MENULIS TEKS EKSPOSISI BERDASARKAN MINAT PESERTA DIDIK KELAS X SMK

    Directory of Open Access Journals (Sweden)

    Wiwit Lili Sokhipah

    2015-03-01

    Full Text Available Tujuan penelitian ini adalah (1 menentukan keefektifan penggunaan model show not tell pada pembelajaran keterampilan menulis teks eksposisi berdasarkan minat peserta didik SMK Kelas X, (2 menentukan keefektifan penggunaan model mind map pada pembelajaran keterampilan menulis teks eksposisi berdasarkan minat peserta didik SMK kelas X, (3 menentukan keefektifan interaksi show not tell dan mind map pada pembelajaran keterampilan menulis teks eksposisi berdasarkan minat peserta didik SMK kelas X. Penelitian ini adalah quasi experimental design (pretes-postes control group design. Dalam desain ini terdapat dua kelompok eksperimen yakni penerapan model show not tell dalam pembelajaran keterampilan menulis teks eksposisipeserta didik dengan minat tinggi dan penerapan model mind map dalam pembelajaran keterampilan menulis teks eksposisi  peserta didik dengan minat rendah. Hasil penelitian adalah (1 model show not tell efektif digunakan  dalam membelajarkan menulis teks eksposisi bagi peserta didik yang memiliki minat tinggi, (2 model mind map efektif digunakan dalam membelajarkan menulis teks eksposisi bagi peserta didik yang memiliki minat rendah, dan (3 model show not tell lebih efektif digunakan dalam membelajarkan menulis teks eksposisi bagi peserta didik yang memiliki minat tinggi, sedangkan model mind map efektif digunakan dalam membelajarkan teks eksposisi pagi peserta didik yang memiliki minat rendah.

  16. PENENTUAN POSISI SUMBER PROSPEK PANAS BUMI BERDASARKAN DATA ANOMALI MAGNETDI DAERAH AKESAHU, PULAU TIDORE, MALUKU UTARA

    Directory of Open Access Journals (Sweden)

    Rohima Wahyu Ningrum

    2015-04-01

    Full Text Available Penelitian geofisika dengan menggunakan metode magnetik yaitu dengan alat Magnetometer Fluxgate. Luas daerah penyelidikan ± 1 km x 1 km, dibatasi oleh koordinat geografis antara 127° 27’ 42” s/d 127° 27’ 43” BT dan 0° 43’ 17” s/d 0° 43’ 19” LU . Penelitian ini bertujuan untuk mengetahui posisi sumber prospek panas bumi di daerah Akesahu, Pulau Tidore berdasarkan data anomali medan magnet. Data medan magnet total yang terukur adalah medan utama, medan luar dan medan anomali. Untuk menghilangkan efek-efek anomali dari medan magnet luar dan anomali medan magnet utama dilakukan perhitungan koreksi variasi harian, koreksi drift (kesalahan alat, koreksi penyesuaian, koreksi IGRF, dan pemisahan anomali residual dan regional. Hasil penelitian menunjukkan bahwa, daerah Akesahu, Pulau Tidore memiliki potensi panas bumi yang masih cukup besar yang ditandai dengan adanya manifestasi panas bumi berupa mata air panas. Anomali medan magnet daerah Akesahu ditunjukkan dengan adanya anomali yang rendah (0.90 sampai dengan -2163.09 nT yang memanjang arah utara-selatan di bagian Timur Pulau Tidore. Disamping itu di sekitar mata air panas Akesahu secara geologis didukung dengan adanya empat buah sesar normal. Ini sebagai sistem pengontrol panas bumi yang lainnya di Pulau Tidore.

  17. Kesehatan Karang di Perairan Kessilampe Kota Kendari Berdasarkan Skor Kesehatan Karang dan Densitas Zooxanthellae

    Directory of Open Access Journals (Sweden)

    Palupi Diyah Ratna

    2017-04-01

    Full Text Available Tujuan dari penelitian ini adalah mengetahui kesehatan karang berdasarkan tutupan persentase karang hidup, skor kesehatan karang, dan densitas zooxanthellae sebagai simbion karang. Metode penentuan titik stasiun dengan menggunakan purposif sample. Metode pengukuran kondisi terumbu karang menggunakan Line Intersept Transect (LIT dengan 3 (tiga kali ulangan tiap stasiun, skor kesehatan karang menggunakan metode quadrat transect (1x1m, dan densitas zooxanthellae dihitung dengan pengambilan sampel jaringan karang. Pengambilan data di perairan menggunakan alat bantu selam SCUBA. Hasil penelitian ini didapat kondisi terumbu karang di lokasi penelitian dalam keadaan rusak dengan rata-rata tutupan karang hidup sebesar 19%. Secara umum biota karang yang mampu bertahan hidup di lokasi penelitian dalam kondisi cukup sehat (skor 4. Hal ini didukung dengan rata-rata jumlah zooxanthellae yang tidak begitu banyak yaitu 1,4 juta sel/cm2 dengan kisaran yang lebar (250ribu-2,5juta sel/cm2. Keberadaan terumbu karang di pantai Mayaria beradaptasi dalam bentuk spot-spot dan didominasi oleh karang foliose dan masive. Kata Kunci : kesehatan karang, Pantai Kessilampe, zooxanthellae

  18. Kompetisi Surat Kabar Lokal Berdasarkan Tingkat Kepuasan Biro Iklan pada Layanan Jasa Media Surat Kabar

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    L. Juni Andi Prasetiya

    2013-11-01

    Full Text Available Abstract: Business newspaper has eliciting competition over source life of supporting media industry, i.e. capital, types of content and types of audience. This research explains the newspaper’s competition based on advertiser satisfaction as a benchmark of media effectiveness. Adopting the theory of uses & gratification and using variables of gratification sought and gratification obtained, these studies examine 5 local newspapers in Yogyakarta. The instrument of competitive strategy is using for further analysis, consisting of superiority direction and superiority magnitude measurements. The results showed that advertising agencies haven’t felt the satisfaction of the services provided by the local newspaper. Key words: competition, gratification sought, gratification obtained, superiority direction, superiority magnitude . Abstrak: Bisnis surat kabar telah memunculkan kompetisi yang memperebutkan sumber penunjang hidup industri media, yakni modal, isi media, dan jenis audiens. Penelitian ini menjelaskan kompetisi tersebut berdasarkan kepuasan pengiklan sebagai tolok ukur efektivitas media. Mengadopsi teori uses & gratification dengan menggunakan variabel gratification sought dan gratification obtained, penelitian ini menguji lima surat kabar lokal di Yogyakara. Selanjutnya dianalisis dengan menggunakan instrumen competitive superiority, terdiri dari superiority direction dan superiority magnitude. Hasil penelitian menunjukkan bahwa biro iklan belum merasakan kepuasan dari layanan yang diberikan oleh jasa surat kabar lokal.

  19. Tinjauan Teologis Tentang Takut Akan Tuhan Berdasarkan Kitab Amsal Dan Implementasinya Dalam Hidup Kekristenan

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    Ril Tampasigi

    2012-04-01

    Full Text Available Sebagai tujuan penelitian adalah Untuk memaparkan konsep Takut akanTUHAN berdasarkan kitab Amsal, Untuk menjelaskan manfaat dari TakutakanTUHAN. Dan Untuk menjelaskan implementasinya dalam kehidupan Kekristenansetiap hari.Adapun metode yang digunakan dalam penelitian ini adalah metode kualitatifyaitu metode penelitian Kepustakaan (library research, terhadap berbagai sumberdata antara lain: Alkitab, tafsiran-tafsiran kitab Amsal dan buku-buku yangmembahas tentang Takut akan TUHAN serta penulis menggunakan metode eksegesis.Yang disusun secara deskriptif untuk mencapai sasaran dan tujuan penulisan.Dalam penelitian ini, ditemukan hasil bahwa Kekristenan seharusnya hidupberdasarkan takut akan TUHAN dengan menyadari akan kemahakuasaan-Nya,kekudusan-Nya, kemahahadiran-Nya dan kemahatahuanNya dalam setiap aspekkehidupan manusia lewat tindakan dan perilaku manusia. Banyak hal dalam duniaini yang akan membuat manusia merasa takut dan gentar, baik itu ketakutanterhadap sesamanya manusia maupun ketakutan terhadap hal-hal yang lainnya.Takutakan TUHAN merupakan suatu perasaan takut yang positif bukan negatif.Takutakan TUHAN bukan seperti perasaan takut yang dialami oleh manusiaterhadap hal-hal yang biasa, tetapi takutakan TUHAN merupakan penghormatanmanusia terhadap TUHAN.

  20. PENENTUAN WAKTU PERAWATAN UNTUK PENCEGAHANPADA KOMPONEN KRITIS CYCLONE FEED PUMP BERDASARKAN KRITERIA MINIMASI DOWN TIME

    Directory of Open Access Journals (Sweden)

    Siti Nandiroh

    2006-08-01

    Full Text Available Sistem perawatan mesin yang dilakukan di PT. Newmont Nusa Tenggara, selama ini masih bersifat korektif yaitu perawatan setelah terjadi kerusakan. Kerusakan komponen ini biasanya akan ditandai dengan ditemukannya produk yang dihasilkan tidak sedikit mengalami kecacatan. Peranan perawatan terhadap komponen-komponen Cyclone Feed Pump pada Process Departement - PT.Newmont Nusa Tenggara sangat penting artinya untuk mencegah terjadinya kecacatan produk masal dan mencegah terjadinya down time produksi. Dan perawatan yang paling baik digunakan adalah perawatan pencegahan sebelum terjadinya kerusakan (preventive maintenance. Mesin kritis adalah mesin yang mengalami frekwensi kerusakan terbesar dengan total downtime terbesar. Untuk penentuan mesin kritis ini, langkah pertama yang dilakukan adalah dengan mengukur lamanya waktu downtime produksi dari tiap-tiap mesin yang ada. Perhitungan MTTR berdasarkan data downtime, yang sebelumnya juga dilakukan uji kecocokan distribusi dan hasilnya sesuai, Dengan melakukan perhitungan Mean Time To Repair dan Mean Time To Failure dapat diketahui rata-rata waktu berapa lama pompa beroperasi dan berapa lama pompa tersebut dapat dilakukan perbaikan serta dapat diketahui Reliability pada Cyclone Feed Pump 2.0.1. Setelah dilakukkan perhitungan, komponen kritis Discharge Pipe pada Cyclone Feed Pump 2.0.1 harus sudah dilakukan inspeksi preventif, karena telah beroperasi 664.8 jam, dan perbaikan yang harus lakukan maksimal 3.4997 jam setiap kali dilakukan shutdown.

  1. DETEKSI DIAMETER TUMOR PADA KULIT MENGGUNAKAN SEGMENTASI CITRA BERDASARKAN KARAKTERISTIK ABCDE

    Directory of Open Access Journals (Sweden)

    Wuwanjie Septian

    2016-09-01

    ABSTRAK Kanker kulit merupakan pertumbuhan sel kulit abnormal yang tidak dapat dikendalikan dan pada stadium lanjut dapat mengakibatkan kematian. Menemukan penyakit ini sedini mungkin merupakan salah satu cara untuk menghindari kecacatan maupun kemungkinan terburuk. Karena letaknya dipermukaan kulit, akan mudah bagi siapa saja untuk mengenali sendiri kanker kulit. Deteksi dini kanker kulit dalam bidang dermatologi, dapat dideteksi berdasarkan karakteristik Asymmetrical Shape, Border, Color, Diameter, Evolution (ABCDE. Dalam penelitian ini, deteksi dini difokuskan pada identifikasi diameter pada 30 citra nevus. Metode penelitian berupa pengolahan citra nevus dengan melakukan konversi citra menjadi citra HSI lalu diubah menjadi citra biner, selanjutnya dilakukan tahap segmentasi menggunakan filter median, proses rekonstruksi morfologi dan pada tahap akhir dilakukan deteksi tepi dengan menggunakan operator sobel. Proses deteksi tepi akan mempermudah menghitung nilai luas diameter nevus. Hasil penelitian deteksi dini kanker kulit terhadap 30 citra nevus, diperoleh hasil bahwa metode pengolahan citra yang diusulkan dapat mendeteksi diameter nevus dan berhasil mengidentifikasi citra tersebut sebagai 26 citra memiliki luas diameter nevus yang diidentifikasi sebagai tumor jinak dan 4 citra nevus yang memiliki diameter > 6 mm dan dinyatakan sebagai tumor melanoma. Kata Kunci: Nevus, Melanoma, Segmentasi, Deteksi Diameter

  2. Kerangka Investigasi Forensik Pada Peladen Pertukaran Berkas Samba Berdasarkan SNI ISO/IEC 27037:2014

    Directory of Open Access Journals (Sweden)

    Dedy Haryadi

    2017-07-01

    Full Text Available Network File System (NFS dan Common Internet File System (CIFS merupakan protokol yang biasa dipakai dalam melakukan pertukaran berkas di dalam sebuah jaringan komputer yang terhubung dengan Network Attached Storage. Mudahnya dalam bertukar berkas dalam sebuah jaringan tidak menutup kemungkinan adanya pertukaran berkas yang bersifat ilegal ataupun berkas yang mengandung tindak kejahatan. Oleh karena itu, diperlukan sebuah model pendekatan investigasi forensik dalam proses pertukaran berkas di sebuah jaringan komputer. Proses akuisisi dalam penelitian ini dilakukan dengan 2 model, yaitu: akuisisi secara langsung pada mesin peladen file-sharing dan akuisisi secara langsung melalui jaringan. Akuisisi secata langsung pada mesin peladen berfokus pada proses akuisisi log dari mesin peladen yang dihasilkan dari layanan samba dan direktori pertukaran berkas. Akusisi secara langsung melalui jaringan berfokus pada direktori pertukaran berkas yang diakses oleh pengguna. Model akuisisi ini berdasarkan SNI ISO/IEC 27037:2014 tentang Pedoman Identifikasi, Pengumpulan, Akuisisi dan Preservasi Bukti Digital yang fokus pada barang bukti elektronik kritis dengan kondisi tidak diperkenankan mati atau shutdown.

  3. Tipologi Klaster Industri Pengolahan Jagung di Kecamatan Merakurak Kabupaten Tuban Berdasarkan Karakteristik Pengembangan Ekonomi Lokalnya

    Directory of Open Access Journals (Sweden)

    Rusyidi Huda Prasetyo

    2014-09-01

    Full Text Available Pengembangan wilayah Kabupaten Tuban khususnya Kecamatan Merakurakurak masih berorientasi pada growth oriented semata. Pontensi jagung yang menjadi komoditas basis pada wilayah tersebut masih belum terkelola dengan optimal, karena tidak adanya arahan yang lebih spesifik terkait diversifikasi dan pengolahan jagung lebih lanjut. Oleh karena itu diperlukan perumusan arahan yang tepat guna dalam mengembangkan industri pengolahan jagung di Kecamatan Merakurak Kabupaten Tuban. Penelitian ini bertujuan mengidentifikasi tipologi klaster industri pengolahan jagung di Kecmatan Merakurak Kabupaten Tuban dengan pendekatan Pengembangan Ekonomi Lokal (PEL. Hal ini dikarenakan konsep PEL sejalan dengan fenomena yang terjadi di wilayah penelitian, yaitu trkait berkembanganya wirausahawan lokal (industri penglahan jagung dalam rangka mengembangkan ekonomi lokal yang mandiri. Terdapat tiga tahapan mencapai tujuan penelitian. Pertama, identifikasi faktor-faktor yang berpengaruh dengan menggunakan metode analisis faktor konfirmatori, selanjutnya mengelompokkan klaster industri berdasarkan faktor-faktor dengan analisis klaster ttersebut dan pada tahap terakhir menginterpretasi analisis klaster pada setiap tipologi yang muncul dengan analisis deskriptif. Adapun hasil yang didapatkan dari penelitian ini adalah tipologi klaster industri yaitu industri intijagung yang berada pada Desa Tuwiri Kulon, Tlogowaru, Tobo, Sugihan. Kemudian industri pemasok yang berada pada Desa Kapu, Tuwiri Wetan, Pongpongan, Temandang, Tegalrejo dan Tahulu. Serta, industri pendukung yang berada pada Desa Mandirejo, Bogorejo, Sumberejo, Sendang Haji, Sambonggede, Sumber, Boreh Bangle, Senori dan Sembungrejo.

  4. RPA70 depletion induces hSSB1/2-INTS3 complex to initiate ATR signaling

    OpenAIRE

    Kar, Ananya; Kaur, Manpreet; Ghosh, Tanushree; Khan, Md. Muntaz; Sharma, Aparna; Shekhar, Ritu; Varshney, Akhil; Saxena, Sandeep

    2015-01-01

    The primary eukaryotic single-stranded DNA-binding protein, Replication protein A (RPA), binds to single-stranded DNA at the sites of DNA damage and recruits the apical checkpoint kinase, ATR via its partner protein, ATRIP. It has been demonstrated that absence of RPA incapacitates the ATR-mediated checkpoint response. We report that in the absence of RPA, human single-stranded DNA-binding protein 1 (hSSB1) and its partner protein INTS3 form sub-nuclear foci, associate with the ATR-ATRIP comp...

  5. Advanced Test Reactor National Scientific User Facility (ATR NSUF) Monthly Report November 2014

    Energy Technology Data Exchange (ETDEWEB)

    Soelberg, Renae [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2014-11-01

    Advanced Test Reactor National Scientific User Facility (ATR NSUF) Monthly Report November 2014 Highlights Rory Kennedy and Sarah Robertson attended the American Nuclear Society Winter Meeting and Nuclear Technology Expo in Anaheim, California, Nov. 10-13. ATR NSUF exhibited at the technology expo where hundreds of meeting participants had an opportunity to learn more about ATR NSUF. Dr. Kennedy briefed the Nuclear Engineering Department Heads Organization (NEDHO) on the workings of the ATR NSUF. • Rory Kennedy, James Cole and Dan Ogden participated in a reactor instrumentation discussion with Jean-Francois Villard and Christopher Destouches of CEA and several members of the INL staff. • ATR NSUF received approval from the NE-20 office to start planning the annual Users Meeting. The meeting will be held at INL, June 22-25. • Mike Worley, director of the Office of Innovative Nuclear Research (NE-42), visited INL Nov. 4-5. Milestones Completed • Recommendations for the Summer Rapid Turnaround Experiment awards were submitted to DOE-HQ Nov. 12 (Level 2 milestone due Nov. 30). Major Accomplishments/Activities • The University of California, Santa Barbara 2 experiment was unloaded from the GE-2000 at HFEF. The experiment specimen packs will be removed and shipped to ORNL for PIE. • The Terrani experiment, one of three FY 2014 new awards, was completed utilizing the Advanced Photon Source MRCAT beamline. The experiment investigated the chemical state of Ag and Pd in SiC shell of irradiated TRISO particles via X-ray Absorption Fine Structure (XAFS) spectroscopy. Upcoming Meetings/Events • The ATR NSUF program review meeting will be held Dec. 9-10 at L’Enfant Plaza. In addition to NSUF staff and users, NE-4, NE-5 and NE-7 representatives will attend the meeting. Awarded Research Projects Boise State University Rapid Turnaround Experiments (14-485 and 14-486) Nanoindentation and TEM work on the T91, HT9, HCM12A and 9Cr ODS specimens has been completed at

  6. Proteomics Reveals Global Regulation of Protein SUMOylation by ATM and ATR Kinases during Replication Stress

    Directory of Open Access Journals (Sweden)

    Stephanie Munk

    2017-10-01

    Full Text Available The mechanisms that protect eukaryotic DNA during the cumbersome task of replication depend on the precise coordination of several post-translational modification (PTM-based signaling networks. Phosphorylation is a well-known regulator of the replication stress response, and recently an essential role for SUMOs (small ubiquitin-like modifiers has also been established. Here, we investigate the global interplay between phosphorylation and SUMOylation in response to replication stress. Using SUMO and phosphoproteomic technologies, we identify thousands of regulated modification sites. We find co-regulation of central DNA damage and replication stress responders, of which the ATR-activating factor TOPBP1 is the most highly regulated. Using pharmacological inhibition of the DNA damage response kinases ATR and ATM, we find that these factors regulate global protein SUMOylation in the protein networks that protect DNA upon replication stress and fork breakage, pointing to integration between phosphorylation and SUMOylation in the cellular systems that protect DNA integrity.

  7. Achieving safety/risk goals for less ATR backup power upgrades

    International Nuclear Information System (INIS)

    Atkinson, S.A.

    1995-01-01

    The Advanced Test Reactor probabilistic risk assessment for internal fire and flood events defined a relatively high risk for a total loss of electric power possibly leading to core damage. Backup power sources were disabled due to fire and flooding in the diesel generator area with propagation of the flooding to a common switchgear room. The ATR risk assessment was employed to define options for relocation of backup power system components to achieve needed risk reduction while minimizing costs. The risk evaluations were performed using sensitivity studies and importance measures. The risk-based evaluations of relocation options for backup power systems saved over $3 million from what might have been otherwise considered open-quotes necessaryclose quotes for safety/risk improvement. The ATR experience shows that the advantages of a good risk assessment are to define risk significance, risk specifics, and risk solutions which enable risk goals to be achieved at the lowest cost

  8. Application of PSA level 1 for the Fugen prototype ATR plant

    International Nuclear Information System (INIS)

    Ikeda, H.; Iguchi, Y.; Sotsu, M.; Seki, O.; Satou, S.

    1997-01-01

    This paper presents application of PSA Level-1 for Prototype Advanced Thermal Reactor Plant ''Fugen'' in consideration of the design characteristics of such reactor and verify the safety aspect of Fugen using thus established procedure, ATR resembles the boiling water reactor (BWR) in a number of points, but there are also some differences between the ATR and the BWR. Therefore. PSA procedure have been established by taking such difference into consideration and by referring to experience of PSA in USA and Japan. Moreover, the core damage frequency was calculated on Fugen by using thus established procedure. As a result, it was verified that results including the maximum value of the uncertainty estimation were found to be quite satisfactory against the target value of reactor damage frequency defined by the International Atomic Energy Agency (IAEA). (author)

  9. Radiation Measurements Laboratory (RML) calibration and assessment of the ATR SPING-3 stack effluent monitor

    International Nuclear Information System (INIS)

    Koeppen, L.D.; Rogers, J.W.; Simpson, O.D.

    1983-12-01

    An evaluation, calibration and assessment of the Eberline SPING-3 ATR stack effluent monitor was conducted. This unit which monitors particulate, iodine and noble gas effluents was producing abnormal results following the initial installation and operational testing. The purposes of this work were to find the causes of the abnormal results and correct them if possible; check the calibrations and adjust them if necessary; and to provide a better in-depth understanding of what the unit is monitoring and how well it performs under this application. Results have shown that there were some problems associated with the unit as initially installed and tested. These problems have been identified and suggested alternatives shown, the monitor was found to be applicable to some extent under the current conditions. The calibrations have been checked and adjustments made. More operation testing and evaluation is needed to assess how well this works under a variety of ATR operating conditions. 2 references, 10 figures, 3 tables

  10. Analysis of European honeybee (Apis mellifera) wings using ATR-FTIR and Raman spectroscopy: A pilot study

    Czech Academy of Sciences Publication Activity Database

    Machovič, Vladimír; Lapčák, L.; Havelcová, Martina; Borecká, Lenka; Novotná, M.; Novotná, M.; Javůrková, I.; Langrová, I.; Hájková, Š.; Brožová, A.; Titěra, D.

    2017-01-01

    Roč. 48, č. 1 (2017), s. 22-29 ISSN 1211-3174 Institutional support: RVO:67985891 Keywords : honeybee wings * ATR-FTIR * Raman spectroscopy * protein * lipid * chitin Subject RIV: CB - Analytical Chemistry, Separation OBOR OECD: Analytical chemistry

  11. Application of FTIR-ATR Spectroscopy for the quality control of wine spirits, brandies and grape marc spirits

    OpenAIRE

    Anjos, O.; Caldeira, I.

    2013-01-01

    This study attempted to apply a rapid method for the determination of methanol, acetaldehyde and ethyl acetate in wine spirits, brandies and grape marc spirits, using Fourier Transform Infrared – Attenuated Total Reflectance (ATR) Spectroscopy.

  12. Diamond-coated ATR prism for infrared absorption spectroscopy of surface-modified diamond nanoparticles

    Czech Academy of Sciences Publication Activity Database

    Remeš, Zdeněk; Kozak, Halyna; Rezek, Bohuslav; Ukraintsev, Egor; Babchenko, Oleg; Kromka, Alexander; Girard, H.A.; Arnault, J.-C.; Bergonzo, P.

    2013-01-01

    Roč. 270, APR (2013), s. 411-417 ISSN 0169-4332 R&D Projects: GA ČR GAP108/12/0910; GA ČR GPP205/12/P331; GA MŠk LH12236; GA MŠk LH12186 Institutional support: RVO:68378271 Keywords : ATR FTIR * CVD * hydrogenation * microwave * nanocrystalline diamond * nanopowder Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 2.538, year: 2013

  13. Proteomics Reveals Global Regulation of Protein SUMOylation by ATM and ATR Kinases during Replication Stress

    DEFF Research Database (Denmark)

    Munk, Stephanie; Sigurðsson, Jón Otti; Xiao, Zhenyu

    2017-01-01

    The mechanisms that protect eukaryotic DNA during the cumbersome task of replication depend on the precise coordination of several post-translational modification (PTM)-based signaling networks. Phosphorylation is a well-known regulator of the replication stress response, and recently an essentia....... They analyze changes in the SUMO and phosphoproteome after MMC and hydroxyurea treatments and find that the DNA damage response kinases ATR and ATM globally regulate SUMOylation upon replication stress and fork breakage....

  14. Applications of diamond crystal ATR FTIR spectroscopy to the characterization of ambers.

    Science.gov (United States)

    Guiliano, Michel; Asia, Laurence; Onoratini, Gérard; Mille, Gilbert

    2007-08-01

    Diamond crystal ATR FTIR spectroscopy is a rapid technique with virtually no sample preparation which requires small sample amounts and showed potential in the study of ambers. FTIR spectra of ambers present discriminating patterns and can be used to distinguish amber from immature resins as copal, to determine local or Baltic origin of archaeological ambers and to detect most of the falsifications encountered in the amber commercialisation.

  15. Gauvain e il corpo smembrato: allegorie nell'«Atre périlleux»

    Directory of Open Access Journals (Sweden)

    Maurizio Virdis

    2013-07-01

    Full Text Available L’Atre Périlleux è un romanzo anonimo francese del XIII secolo e fa parte della corrente letteraria che rimettere in questione il romanzo cortese di tradizione chrestieniana. Piú che all’ideologia o alle istanze della classe cavalleresca e della nobiltà minore, il nostro romanzo risponde alle prospettive e alle necessità intellettuali della clergie, che si fa gioco dei valori cavallereschi tradizionali. L’Atre Périlleux è condotto, con toni di humour e una trama complessa, su binari metaletterari e allegorici (di cui il corpo smembrato di Gauvain è il simbolo piú eminente, e si pone come critica della decadenza della società cortese, animato però dal proposito del rinnovamento morale di essa.L’Atre Périlleux is an anonymous French romance of the thirteenth century and it is part of the literary movement that calls into question the tradition of courtly romance dating back to Chrétien de Troyes. More than to the ideology and instances of the class of minor nobility and chivalry, our romance responds to the clergie’s needs and intellectual perspectives, and it mocks the traditional values of chivalry. L’Atre Périlleux is conducted, with a hint of humour and a complicated plot, on metaliterary and allegorical tracks (and Gauvain’s dismembered body is the most prominent symbol of this allegorical tone, so that our romance stands as a critique of courtly society decadence, nevertheless animated by the purpose of its moral renewal.

  16. ATR-FTIR for rapid detection and quantification of counterfeit medicines

    OpenAIRE

    Ogwu, John; Lawson, Graham; Tanna, Sangeeta

    2015-01-01

    From therapeutic to lifestyle medicines, the counterfeiting of medicines has been on the rise in recent times [1]. Estimates indicate that about 10% of medicines worldwide are counterfeits with much higher figures in developing countries [2]. Currently, the rapid screening of medicines is a challenge leaving many patients at risk [1]. This study considered the potential use of Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) for rapid quantitative analysis of ta...

  17. Trasmission and Reflection (ATR)Far-Infrared Spectroscopy Applied in the Analysis of Cultural Heritage Materials

    OpenAIRE

    Kendix, Elsebeth Langholz

    2009-01-01

    FIR spectroscopy is an alternative way of collecting spectra of many inorganic pigments and corrosion products found on art objects, which is not normally observed in the MIR region. Most FIR spectra are traditionally collected in transmission mode but as a real novelty it is now also possible to record FIR spectra in ATR (Attenuated Total Reflectance) mode. In FIR transmission we employ polyethylene (PE) for preparation of pellets by embedding the sample in PE. Unfortunately, ...

  18. The Conference Proceedings of the 2003 Air Transport Research Society (ATRS) World Conference, Volume 3

    Science.gov (United States)

    Bowen, Brent (Editor); Gudmundsson, Sveinn (Editor); Oum, Tae (Editor)

    2003-01-01

    Volume 3 of the 2003 Air Transport Reserch Society (ATRS) World Conference includes papers on topics relevant to airline operations worldwide. Specific topics include: European Union and civil aviation regimens;simulating decision making in airline operations, passenger points of view on convenient airports; route monopolies and nonlinear pricing; cooperation among airports in Europe; fleet modernizaiton in Brazil;the effects of deregulation on the growth of air transportation in Europe and the United States.

  19. Fission yeast shelterin regulates DNA polymerases and Rad3(ATR kinase to limit telomere extension.

    Directory of Open Access Journals (Sweden)

    Ya-Ting Chang

    2013-11-01

    Full Text Available Studies in fission yeast have previously identified evolutionarily conserved shelterin and Stn1-Ten1 complexes, and established Rad3(ATR/Tel1(ATM-dependent phosphorylation of the shelterin subunit Ccq1 at Thr93 as the critical post-translational modification for telomerase recruitment to telomeres. Furthermore, shelterin subunits Poz1, Rap1 and Taz1 have been identified as negative regulators of Thr93 phosphorylation and telomerase recruitment. However, it remained unclear how telomere maintenance is dynamically regulated during the cell cycle. Thus, we investigated how loss of Poz1, Rap1 and Taz1 affects cell cycle regulation of Ccq1 Thr93 phosphorylation and telomere association of telomerase (Trt1(TERT, DNA polymerases, Replication Protein A (RPA complex, Rad3(ATR-Rad26(ATRIP checkpoint kinase complex, Tel1(ATM kinase, shelterin subunits (Tpz1, Ccq1 and Poz1 and Stn1. We further investigated how telomere shortening, caused by trt1Δ or catalytically dead Trt1-D743A, affects cell cycle-regulated telomere association of telomerase and DNA polymerases. These analyses established that fission yeast shelterin maintains telomere length homeostasis by coordinating the differential arrival of leading (Polε and lagging (Polα strand DNA polymerases at telomeres to modulate Rad3(ATR association, Ccq1 Thr93 phosphorylation and telomerase recruitment.

  20. Rabi like angular splitting in Surface Plasmon Polariton - Exciton interaction in ATR configuration

    Science.gov (United States)

    Hassan, Heba; Abdallah, T.; Negm, S.; Talaat, H.

    2018-05-01

    We have studied the coupling of propagating Surface Plasmon Polaritons (SPP) on silver films and excitons in CdS quantum dots (QDs). We employed the Kretschmann-Raether configuration of the attenuated total reflection (ATR) to propagate the SPP on silver film of thickness 47.5 nm at three different wavelengths. The CdS QD have been chemically synthesized with particular size such that its exciton of energy would resonate with SPP. High resolution transmission electron microscopy (HRTEM) and scan tunneling microscopy (STM) were used to measure the corresponding QDs size and confirm its shape. Further confirmation of the size has been performed by the effective mass approximation (EMA) model utilizing the band gap of the prepared QDs. The band gaps have been measured through UV-vis absorption spectra as well as scan tunneling spectroscopy (STS). The coupling has been observed as two branching dips in the ATR spectra indicating Rabi like splitting. To the best of our knowledge, this is the first time that Rabi interaction is directly observed in an ATR angular spectra. This observation is attributed to the use a high resolution angular scan (±0.005°), in addition to the Doppler width of the laser line as well as the energy distribution of the excitons. The effect of three different linker molecules (TOPO, HDA), (Pyridine) and (Tri-butylamine) as surface ligands, on SPP-Exciton interaction has been examined.

  1. Inherited germline ATRX mutation in two brothers with ATR-X syndrome and osteosarcoma.

    Science.gov (United States)

    Ji, Jianling; Quindipan, Catherine; Parham, David; Shen, Lishuang; Ruble, David; Bootwalla, Moiz; Maglinte, Dennis T; Gai, Xiaowu; Saitta, Sulagna C; Biegel, Jaclyn A; Mascarenhas, Leo

    2017-05-01

    We report a family in which two brothers had an undiagnosed genetic disorder comprised of dysmorphic features, microcephaly, severe intellectual disability (non-verbal), mild anemia, and cryptorchidism. Both developed osteosarcoma. Trio exome sequencing (using blood samples from the younger brother and both parents) was performed and a nonsense NM_000489.4:c.7156C>T (p.Arg2386*) mutation in the ATRX gene was identified in the proband (hemizygous) and in the mother's peripheral blood DNA (heterozygous). The mother is healthy, does not exhibit any clinical manifestations of ATR-X syndrome and there was no family history of cancer. The same hemizygous pathogenic variant was confirmed in the affected older brother's skin tissue by subsequent Sanger sequencing. Chromosomal microarray studies of both brothers' osteosarcomas revealed complex copy number alterations consistent with the clinical diagnosis of osteosarcoma. Recently, somatic mutations in the ATRX gene have been observed as recurrent alterations in both osteosarcoma and brain tumors. However, it is unclear if there is any association between osteosarcoma and germline ATRX mutations, specifically in patients with constitutional ATR-X syndrome. This is the first report of osteosarcoma diagnosed in two males with ATR-X syndrome, suggesting a potential increased risk for cancer in patients with this disorder. © 2017 Wiley Periodicals, Inc.

  2. Diamond-coated ATR prism for infrared absorption spectroscopy of surface-modified diamond nanoparticles

    Science.gov (United States)

    Remes, Z.; Kozak, H.; Rezek, B.; Ukraintsev, E.; Babchenko, O.; Kromka, A.; Girard, H. A.; Arnault, J.-C.; Bergonzo, P.

    2013-04-01

    Linear antenna microwave chemical vapor deposition process was used to homogeneously coat a 7 cm long silicon prism by 85 nm thin nanocrystalline diamond (NCD) layer. To show the advantages of the NCD-coated prism for attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) of nanoparticles, we apply diamond nanoparticles (DNPs) of 5 nm nominal size with various surface modifications by a drop-casting of their methanol dispersions. ATR-FTIR spectra of as-received, air-annealed, plasma-oxidized, and plasma-hydrogenated DNPs were measured in the 4000-1500 cm-1 spectral range. The spectra show high spectral resolution, high sensitivity to specific DNP surface moieties, and repeatability. The NCD coating provides mechanical protection against scratching and chemical stability of the surface. Moreover, unlike on bare Si surface, NCD hydrophilic properties enable optically homogeneous coverage by DNPs with some aggregation on submicron scale as evidenced by scanning electron microscopy and atomic force microscopy. Compared to transmission FTIR regime with KBr pellets, direct and uniform deposition of DNPs on NCD-ATR prism significantly simplifies and speeds up the analysis (from days to minutes). We discuss prospects for in situ monitoring of surface modifications and molecular grafting.

  3. Diamond-coated ATR prism for infrared absorption spectroscopy of surface-modified diamond nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Remes, Z., E-mail: remes@fzu.cz [Institute of Physics of the ASCR, v.v.i., Cukrovarnicka 10, Praha 6 (Czech Republic); Kozak, H.; Rezek, B.; Ukraintsev, E.; Babchenko, O.; Kromka, A. [Institute of Physics of the ASCR, v.v.i., Cukrovarnicka 10, Praha 6 (Czech Republic); Girard, H.A.; Arnault, J.-C.; Bergonzo, P. [CEA, LIST, Diamond Sensors Laboratory, F-91191 Gif-sur-Yvette (France)

    2013-04-01

    Linear antenna microwave chemical vapor deposition process was used to homogeneously coat a 7 cm long silicon prism by 85 nm thin nanocrystalline diamond (NCD) layer. To show the advantages of the NCD-coated prism for attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) of nanoparticles, we apply diamond nanoparticles (DNPs) of 5 nm nominal size with various surface modifications by a drop-casting of their methanol dispersions. ATR-FTIR spectra of as-received, air-annealed, plasma-oxidized, and plasma-hydrogenated DNPs were measured in the 4000–1500 cm{sup −1} spectral range. The spectra show high spectral resolution, high sensitivity to specific DNP surface moieties, and repeatability. The NCD coating provides mechanical protection against scratching and chemical stability of the surface. Moreover, unlike on bare Si surface, NCD hydrophilic properties enable optically homogeneous coverage by DNPs with some aggregation on submicron scale as evidenced by scanning electron microscopy and atomic force microscopy. Compared to transmission FTIR regime with KBr pellets, direct and uniform deposition of DNPs on NCD-ATR prism significantly simplifies and speeds up the analysis (from days to minutes). We discuss prospects for in situ monitoring of surface modifications and molecular grafting.

  4. Analysis of cosmetic residues on a single human hair by ATR FT-IR microspectroscopy.

    Science.gov (United States)

    Pienpinijtham, Prompong; Thammacharoen, Chuchaat; Naranitad, Suwimol; Ekgasit, Sanong

    2018-05-15

    In this work, ATR FT-IR spectra of single human hair and cosmetic residues on hair surface are successfully collected using a homemade dome-shaped Ge μIRE accessary installed on an infrared microscope. By collecting ATR spectra of hairs from the same person, the spectral patterns are identical and superimposed while different spectral features are observed from ATR spectra of hairs collected from different persons. The spectral differences depend on individual hair characteristics, chemical treatments, and cosmetics on hair surface. The "Contact-and-Collect" technique that transfers remarkable materials on the hair surface to the tip of the Ge μIRE enables an identification of cosmetics on a single hair. Moreover, the differences between un-split and split hairs are also studied in this report. These highly specific spectral features can be employed for unique identification or for differentiation of hairs based on the molecular structures of hairs and cosmetics on hairs. Copyright © 2018 Elsevier B.V. All rights reserved.

  5. Pressure-modulation dynamic attenuated-total-reflectance (ATR) FT-IR spectroscopy

    Science.gov (United States)

    Marcott, C.; Story, G. M.; Noda, I.; Bibby, A.; Manning, C. J.

    1998-06-01

    A single-reflectance attenuated-total-reflectance (ATR) accessory with a diamond internal-reflection element was modified by the addition of a piezoelectric transducer. Initial dynamic pressure-modulation experiments have been performed in the sample compartment of a step-scanning FT-IR spectrometer. A sinusoidal pressure modulation applied to samples of isotactic polypropylene and linear low density polyethylene resulted in dynamic responses which appear to be similar to those observed in previous dynamic 2D IR experiments. Preliminary pressure-modulation dynamic ATR results are also reported for a styrene-butadiene-styrene triblock copolymer. The new method has the advantages that a much wider variety of sample types and geometries can be studied and less sample preparation is required. Dynamic 2D IR experiments carried out by ATR no longer require thin films of large area and sufficient strength to withstand the dynamic strain applied by a rheometer. The ability to obtain dynamic IR spectroscopic information from a wider variety of sample types and thicknesses would greatly expand the amount of useful information that could be extracted from normally complicated, highly overlapped IR spectra.

  6. PERKIRAAN PADAT PENEBARAN IKAN KERAPU MACAN (Epinephelus fuscoguttatus YANG OPTIMUM BERDASARKAN PADA KEBUTUHAN OKSIGEN TERLARUT

    Directory of Open Access Journals (Sweden)

    Arif Dwi Santoso

    2016-11-01

    Full Text Available Studi tentang perkiraan padat penebaran optimum pada ikan kerapu macan (Epinephelus fuscoguttatus berdasarkan pada kebutuhan oksigen terlarut telah dilakukan pada areal Teluk Hurun, Lampung pada bulan Juli 2003. Rata-rata hasil pengukuran kualitas lingkungan keramba jaring apung 13,5 m3 yang berisi ikan kerapu macan meliputi oksigen terlarut 3,68—6,76 (5,35 ± 0,25 mg/L suhu air 28,64—29,72 (29,09 ± 0,09 oC. Salinitas 32,69—33,0 (32,94 ± 0,13 psu and turbiditas 0,27—13,62 (1,15 ± 0,41 NTU. Data tambahan meliputi laju pemasukan air sungai sekitar 0,02 m3/detik dan kecepatan arus 2,3—5,1 cm/detik. Dari analisis data laju respirasi ikan kerapu macan yang diukur dengan mengunakan fish chamber menghasilkan fungsi korelasi terhadap bobot badan ikan yaitu Y= 0,0038 + 0,6108 dengan R2= 0,7437 dan fungsi padat penebaran yang optimum yaitu Y= 1505,6 X -0.632; R2= 0,7419. Dari kedua fungsi tersebut didapatkan rekomendasi penentuan padat penebaran yang optimum berdasarkan kebutuhan oksigen terlarut dengan batasan sebagai berikut: stok ikan berukuran kurang dari 50 g disarankan ditebar dengan kepadatan 175 ekor/m3, ikan ukuran 100--200 g sekitar 63 ekor/m3 dan ikan ukuran lebih dari 1.200 g sekitar 17 ekor/m3. Study on the optimum stocking rate estimation on tiger grouper (Epinephelus fuscoguttatus base on dissolved oxygen budged was held in aquaculture area Hurun Bay Lampung in July 2003. Environmental parameters of Hurun Bay estuary were monitored at the site of 13.5 m3 floating net cages for E. fuscoguttatus in July 2003. Average dissolved oxygen inside cages was 3.68—6.76 (5.35 ± 0.25 mg/L with water temperature 28.64—29.72 (29.09 ± 0.09 oC. Salinity 32.69—33.0 (32.94 ± 0.13 psu and turbidity 0.27—13.62 (1.15 ± 0.41 NTU. Inflow rate of cages was 0.02 m3/sec with current velocity 2.3—5.1 cm/s. Results showed that the functions of respiration rate for correlation between respiration of E. fucoguttatus and their weight was Y= 0

  7. Diversitas Genetik Anopheles balabacensis, Baisas di Berbagai Daerah Indonesia Berdasarkan Sekuen Gen ITS 2 DNA Ribosom

    Directory of Open Access Journals (Sweden)

    Widiarti Widiarti

    2016-05-01

    dilaporkan sebagai spesies kompleks di berbagai negara, akan tetapi belum banyak dilaporkan di Indonesia. Penanggulangan malaria agar lebih efektif perlu adanya perbaikan dan pendekatan strategi dalam pengendalian vektor, termasuk sangat diperlukan adanya pemahaman terhadap spesies dan bioekologinya. Tujuan penelitian adalah untuk : a. Mengidentifikasi secara molekuler nyamuk An. balabacensis yang dicurigai sebagai spesies kompleks berdasarkan sekuen ITS2 DNA ribosom, b. Mengetahui diversitas genetik nyamuk An. balabacensis dari daerah endemis dan non endemis dengan jarak geografis yang berbeda, c. Mengetahui kekerabatan genetik (jarak taksonomi nyamuk An. balabacensis dari berbagai daerah di Indonesia dengan merekonstruksi pohon filogenetik. Hasil penelitian menunjukkan bahwa An. balabacensis di Indonesia merupakan spesies kompleks simpatrik dan allopatrik. Ada perbedaan kekerabatan genetikyang cukup jauh diantara populasi An. balabacensis di Pusuk Lestari, wilayah Puskesmas Meninting, Lombok Barat, NTB yang merupakan simpatrik kompleks. Berdasarkan hubungankekerabatan An. leucosphyrus group, An. balabacensis dari Berjoko, Kabupaten Nunukan menunjukkan kecenderungan terpisah cukup jauh dibandingkan dengan An. balabacensiskompleks lainnya yang berasal dari Jawa Tengah dan Lombok, NTB.Kata kunci : An. balabacensis, variasi genetik, ITS2 DNA ribosom

  8. KECEMASAN MATEMATIK SISWA KELAS XI SMK BERDASARKAN MAHMOOD DAN KHATOON DALAM SETTING PROBLEM BASED LEARNING

    Directory of Open Access Journals (Sweden)

    Desy Kumalasari

    2017-03-01

    Full Text Available Penelitian ini bertujuan untuk mendeskripsikan kualitas pembelajaran matematika dalam setting problem based learning terhadap kemampuan pemecahan masalah siswa kelas XI SMK, mendeskripsikan tingkat kecemasan matematik siswa dalam mengikuti pelajaran matematika dalam setting problem based learning, dan mendeskripsikan kemampuan pemecahan masalah berdasarkan tingkat kecemasan matematik. Metode penelitian ini adalah mixed methods. Subjek dalam penelitian ini adalah siswa kelas XI TKKB SMKN 10 Semarang. Selanjutnya dipilih 6 siswa dari masing-masing kemampuan pemecahan masalah berdasarkan tingkat kecemasan matematik. Metode pengumpulan data dalam penelitian ini adalah metode observasi, dokumentasi, tes, dan wawancara. Analisis data dalam penelitian ini adalah analisis kualitas pembelajaran, analisis tingkat kecemasan matematik, analisis kemampuan pemecahan masalah, reduksi data, penyajian data, dan menarik kesimpulan dan verifikasi. Hasil penelitian ini diperoleh: kualitas pembelajaran dalam setting problem based learning dalam kategori baik, tingkat kecemasan matematik siswa kelas XI SMKN 10 Semarang sebelum pembelajaran matematika adalah rendah, pada saat kegiatan pembelajaran adalah tinggi, dan setelah kegiatan pembelajaran adalah rendah, untuk tingkat kecemasan sebelum tes kemampuan pemecahan masalah adalah rendah, dan setelah tes kemampuan pemecahan masalah adalah tinggi, dan kemampuan pemecahan masalah matematika siswa yang tingkat kecemasan matematik rendah lebih baik dari pada siswa yang tingkat kecemasan matematiknya tinggi.   This research aimed to describe the quality of  mathematics teaching in the setting of problem based learning to problem­solving ability of class XI student of SMK, the level of mathematics  anxiety  of   students  in  participating  in  the  setting  math  problem  based learning, and the problem­solving abilities by mathematics anxiety levels. This research method is mixed methods. Subjects in this

  9. METODE DATA MINING UNTUK KLASIFIKASI DATA SEL NUKLEUS DAN SEL RADANG BERDASARKAN ANALISA TEKSTUR

    Directory of Open Access Journals (Sweden)

    Toni Arifin

    2016-03-01

    Full Text Available ABSTRACT - The Pap Smear test is done to see the presence of infection or changes in cells that can turn into cancer cells. In this research is using data on analysis results of texture image processing on previous research that is using a nucleus cell and inflammation cell in the image Pap Smear cell. The purpose of this research is to find the best method for classifying the nucleus cell and inflammation cell based on texture analysis GLCM (Gray Level Co-occurrence Matrix in this research used of method Decision tree (C 4.5, Naive Bayes and k-Nearest Neighbour. The results of this research brings about the best methods for classification of the data nucleus cell and inflammation cell that is a method of Decision tree (C4.5 with accuracy 97,56% whereas results for Naive Bayes 90,89% and k-Nearest Neighbour 95,97%. Keywords: Data mining, classification, Pap Smear cell, Texture Analysis ABSTRAKSI - Tes Pap Smear dilakukan untuk melihat adanya infeksi atau perubahan sel-sel yang dapat berubah menjadi sel kanker. Pada penelitian ini menggunakan data analisis tekstur yang didapatkan dari hasil pengolahan citra pada penelitian sebelumnya yaitu menggunakan sel nukleus dan sel radang pada citra sel Pap Smear. Tujuan dari penelitian ini adalah mencari metode terbaik untuk mengklasifikasikan sel nukleus dan sel radang berdasarkan analisa teksur GLCM (Gray Level Co-occurrence Matrix Metode yang digunakan dalam penelitian ini adalah metode Decision tree (C4.5, Naive Bayes dan k-Nearest Neighbour. Hasil dari penelitian ini didapatkan metode terbaik untuk klasifikasi data sel nukleus dan sel radang yaitu metode Decision tree (C4.5 dengan akurasi 97,56% sedangkan hasil untuk Naive Bayes 90,89% dan k-Nearest Neighbour 95,97%. Kata Kunci: Data mining, Klasifikasi, Sel Pap Smear, Analisa Tekstur

  10. Analisis Keandalan Struktur Padeye Berdasarkan Konfigurasi Rigging pada Lifting Upper Deck Modul Modec dengan Pendekatan Dinamik

    Directory of Open Access Journals (Sweden)

    Iqbal Gayuh Raharjaning Mukti Sumarsono

    2017-01-01

    Full Text Available Di dalam perusahaan yang bergerak dalam bidang fabrikasi, lifting merupakan salah satu tahapan terpenting dalam membangun bangunan lepas pantai sehingga diperlukan perhitungan dan analisis tambahan untuk memperhatikan apakah struktur tersebut aman ataupun tidak. Sekenario lifting berdasarkan konfigurasi rigging dapat dibuat untuk menentukan rigging equipment yang tepat. Pada kasus ini, struktur yang di-lifting adalah struktur upper deck dari modul MODEC yang dikerjakan oleh PT. GPS Batam. Deck ini memiliki panjang sekitar 19,5 m dan lebar 9 m. Berat total struktur dengan equipment sebesar 55 ton. Konfigurasi rigging yang akan digunakan ada tiga, yaitu Model A (tanpa spreader bar, Model B (satu spreader bar, dan Model C (dua spreader bar. Dari ketiga konfigurasi tersebut akan diperoleh dimensi padeye yang berbeda- beda. Model A, Model B, dan Model C memiliki diameter hole padeye masing-masing sebesar 74 mm, 65 mm, dan 52 mm. Tegangan dan UC tertinggi sama- sama terjadi pada Model A sebesar 17625,99 psi dan untuk UC sebesar 0,52. Tetapi hal ini berbanding terbalik apabila ditinjau dari daerah lubang. Tegangan ataupun UC yang lebih tinggi jatuh pada Model C yang menggunakan dua spreader bar sebesar 13936,58 psi dan nilai UC sebesar 0,45. Tegangan yang terjadi di attachments maupun sekitar hole dijadikan variabel dalam mencari keandalan yang terjadi pada tiap padeye dengan metode Mean value first order second moment (MVFOSM. Sehingga didapatkan Probality of Failure (PoF pada attachments padeye pada Model A, B, dan C masing- masing sebesar 0,126%, 0,064 %, dan 0,0001 %. Sedangkan PoF pada hole padeye sebesar 0,062% untuk Model A, 0,10 % untuk Model B, dan 0,23% untuk Model C.

  11. KERAGAAN WARNA IKAN CLOWN BIAK (Amphiprion percula POPULASI ALAM DAN BUDIDAYA BERDASARKAN ANALISIS GAMBAR DIGITAL

    Directory of Open Access Journals (Sweden)

    Ruby Vidia Kusumah

    2015-09-01

    Full Text Available Penelitian ini bertujuan untuk mengkaji keragaan warna ikan clown Biak (Amphiprion percula populasi alam dan budidaya berdasarkan analisis gambar digital sebagai dasar upaya pemuliaannya. Gambar digital diambil dari koleksi ikan clown Biak Balai Besar Perikanan Budidaya Laut Lampung, Balai Besar Penelitian dan Pengembangan Budidaya Laut Gondol, Bali; serta pengumpul ikan hias di Denpasar, Bali menggunakan kamera digital Canon EOS 600D. Pola warna dikarakterisasi secara visual terhadap variasi strip hitam dan putih pada dasar badan oranye, jenis warna dianalisis menggunakan ImageJ 1.49s, persentase penutupan warna dilakukan dengan Adobe Photoshop CS5. Pola warna dikarakterisasi oleh strip hitam tebal, tipis, gelap, pudar, terputus, bergabung, serta strip putih normal, pelana, spot, melebar, dan terputus. Warna hitam alam dikarakterisasi oleh hue (H: 300-60º, saturation (S: 8%-56%, brightness (B: 3%-19%, sedangkan budidaya H: 300-23º, S: 9%-71%, B: 4%-20%. Warna oranye alam H: 19-33º, S: 88%-98%, B: 47%-85%, dan budidaya H: 14-29º, S: 86%-99%, B: 38%-82%. Warna putih alam H: 36-270º; S: 1%-13%, B: 66%-88%, dan budidaya H: 0-229º, S: 0%-14%, B: 55%-87%. Persentase penutupan warna badan didominasi warna oranye dengan rata-rata 45% untuk populasi alam dan 57% untuk populasi budidaya. Keragaan warna ikan clown Biak dapat diarahkan pada pembentukan strain misbar, picasso, spot (domino, dan onyx. Metode analisis gambar digital sangat potensial digunakan untuk analisis keragaan warna ikan hias.

  12. Analisis Perbandingan KNN dengan SVM untuk Klasifikasi Penyakit Diabetes Retinopati berdasarkan Citra Eksudat dan Mikroaneurisma

    Directory of Open Access Journals (Sweden)

    SUCI AULIA

    2015-01-01

    Full Text Available ABSTRAK Penelitian mengenai pengklasifikasian tingkat keparahan penyakit Diabetes Retinopati berbasis image processing masih hangat dibicarakan, citra yang biasa digunakan untuk mendeteksi jenis penyakit ini adalah citra optik disk, mikroaneurisma, eksudat, dan hemorrhages yang berasal dari citra fundus. Pada penelitian ini telah dilakukan perbandingan algoritma SVM dengan KNN untuk klasifikasi penyakit diabetes retinopati (mild, moderate, severe berdasarkan citra eksudat dan microaneurisma. Untuk proses ekstraksi ciri digunakan metode wavelet  pada masing-masing kedua metode tersebut. Pada penelitian ini digunakan 160 data uji, masing-masing 40 citra untuk kelas normal, kelas mild, kelas moderate, kelas saviere. Tingkat akurasi yang diperoleh dengan menggunakan metode KNN lebih tinggi dibandingkan SVM, yaitu 65 % dan 62%. Klasifikasi dengan algoritma KNN diperoleh hasil terbaik dengan parameter K=9 cityblock. Sedangkan klasifikasi dengan metode SVM diperoleh hasil terbaik dengan parameter One Agains All. Kata kunci: Diabetic Retinopathy, KNN , SVM, Wavelet.   ABSTRACT Research based on severity classification of the disease diabetic retinopathy by using image processing method is still hotly debated, the image is used to detect the type of this disease is an optical image of the disk, microaneurysm, exudates, and bleeding of the image of the fundus. This study was performed to compare SVM method with KNN method for classification of diabetic retinopathy disease (mild, moderate, severe based on exudate and microaneurysm image. For feature extraction uses wavelet method, and each of the two methods. This study made use of 160 test data, each of 40 images for normal class, mild class, moderate class, severe class. The accuracy obtained by KNN higher than SVM, with 65% and 62%. KNN classification method achieved the best results with the parameters K = 9, cityblock. While the classification with SVM method obtained the best results with

  13. REKONSTRUKSI KEWENANGAN PENYIDIKAN BADAN NARKOTIKA NASIONAL BERDASARKAN UNDANG-UNDANG NOMOR 35 TAHUN 2009 TENTANG NARKOTIKA

    Directory of Open Access Journals (Sweden)

    M. Sahid

    2016-02-01

    Full Text Available Abstract Authority to conduct criminal investigations of narcotic regulated under Law No. 35 of 2009 on Narcotics, namely article 81 of the law. Dibeikan investigative authority to the national drug agencies and the police of the republic of Indonesia. With the division of the investigative authority of overlap and multiple interpretations of article 81 of Law No 35 of 2009 on Narcotics. This paper aims to analyze the rules of authority narcotics investigation of criminal offenses. This paper is based on research using the normative approach to law (statute approach and the conceptual approach (conceptual approach. The results showed that the regulation of narcotic crime investigation authority should be clarified and given limit, so in practice the rule of law is not a clash of authority between the National Narcotics Agency to police the Republic of Indonesia. As well as law enforcement agencies in carrying out their duties effectively and goes in accordance with the criminal justice system. Key words: authority investigation, crime, narcotics   Abstrak Kewenangan melakukan penyidikan tindak pidana narkotika diatur didalam Undang-undang Nomor 35 Tahun 2009 tentang Narkotika yaitu Pasal 81 Undang-undang tersebut. Kewenangan penyidikan diberikan kepada Badan Narkotika Nasional dan Kepolisian Republik Indonesia. Dengan adanya pembagian kewenangan penyidikan tersebut terjadi tumpang tindih dan multi tafsir terhadap Pasal 81 Undang-undang Nomor 35 Tahun 2009tentang Narkotika. Tulisan ini bertujuan untuk menganalisis aturan tentang kewenangan penyidikan terhadap tindak pidana narkotika. Tulisan ini dibuat berdasarkan penelitian normatif dengan menggunakan pendekatan perundang-undangan (Statute Approach dan pendekatan konsep (Conceptual Approach. Hasil penelitian menunjukkan bahwa pengaturan mengenai kewenangan penyidikan tindak pidana narkotika harus diperjelas dan diberi batasan, sehingga dalam praktek penegakan hukum tidak terjadi benturan

  14. Small-molecule inhibitors of Ataxia Telangiectasia and Rad3 related kinase (ATR) sensitize lymphoma cells to UVA radiation

    DEFF Research Database (Denmark)

    Biskup, Edyta; Naym, David Gram; Gniadecki, Robert

    2016-01-01

    inhibited by small molecule antagonists VE-821, VE-822 or Chir-124, or by small interfering RNAs (siRNAs). Cell cycle and viability were assessed by flow cytometry. RESULTS: Small molecule inhibitors of ATR and Chk1 potently sensitized all cell lines to PUVA and, importantly, also to UVA, which by itself...... did not cause apoptotic response. VE-821/2 blocked ATR pathway activation and released the cells from the G2/M block caused by UVA and PUVA, but did not affect apoptosis caused by other chemotherapeutics (etoposide, gemcitabine, doxorubicine) or by hydrogen peroxide. Knockdown of ATR and Chk1 with si......RNA also blocked the ATR pathway and released the cells from G2/M block but did not sensitize the cells to UVA as observed with the small molecule inhibitors. The latter suggested that the synergism between VE-821/2 or Chir-124 and UVA was not solely caused by specific blocking of ATR kinase but also ATR...

  15. Perbedaan kadar glukosa darah puasa pasien diabetes melitus berdasarkan pengetahuan gizi, sikap dan tindakan di poli penyakit dalam rumah sakit islam jakarta

    Directory of Open Access Journals (Sweden)

    Ahmad Syauqy

    2016-03-01

    Full Text Available Latar belakang :Perilaku makan yang tidak tepat dapat menyebabkan meningkatnya kadar glukosa darah penderita diabetes. Perilaku dibagi menjadi tiga domain yaitu pengetahuan, sikap dan tindakan. Tujuan penelitian :Melihat perbedaan kadar glukosa darah berdasarkan pengetahuan gizi, sikap dan tindakan pasien diabetes melitus tipe 2. Metode analisis : Jenis penelitian ini adalah penelitian yang bersifat deskriptif analitik dengan pendekatan crosssectional. Sampel sebanyak 50 responden, dalam penelitian ini adalah pasien diabetes melitus yang menjalani pemeriksaan kadar glukosa darah di Poli Penyakit Dalam Rumah Sakit Islam Jakarta. Pengetahuan gizi diukur berdasarkan indikator pengertian penyakit diabetes, prinsip dan syarat diet, penukar bahan makanan dan aktivitas atau olahraga ; tingkat sikap diukur berdasarkan indicator pemeriksaan penyakit diabetes, aktifitas/olahraga, kesadaran diet, bahan makanan dan jumlah porsi ; tingkat tindakan diukur berdasarkan indikator pemilihan makanan/minuman, kebiasaan makan, jadwal makan, aktifitas/olahraga dan bahan makanan penukar. Pengujian One Way Anova digunakan untuk menjawab pertanyaan penelitian. Hasil :Sebanyak 12 responden (24% memiliki pengetahuan gizi kurang, 14 responden (28% memiliki sikap yang kurang baik, 8 responden (16% memiliki tindakan kurang. Rata – rata glukosa darah berbeda menurut tingkat pengetahuan (F = 4,448 ; p = 0,017, sikap (F = 3,930 ; p = 0,026 dan tindakan (F = 3,427 ; p = 0,041. Simpulan :Ada perbedaan bermakna kadar glukosa darah berdasarkan pengetahuan gizi, sikap dan perilaku terhadap pasien dengan diabetes mellitus tipe 2 di Rumah Sakit Islam Jakarta. Diperlukan  penelitian tentang pengaruh konseling gizi terhadap perilaku pasien agar dapat mengetahui keberhasilan Dietisien.

  16. Perjanjian Berbahasa Asing yang Dibuat Oleh Notaris Berdasarkan Undang-Undang Nomor 30 Tahun 2004 Tentang Jabatan Notaris

    Directory of Open Access Journals (Sweden)

    Aliya Sandra Dewi

    2016-04-01

    Full Text Available Perjanjian Berbahasa Asing yang Dibuat Notaris Berdasarkan Undang-Undang Nomor 30 Tahun 2004. Posisi notaris sebagai fungsionaris dalam masyarakat dianggap sebagai tempat resmi yang dapat memperoleh saran. Segala sesuatu yang ditulis dan ditetapkan sudah benar dan pejabat produsen resmi dalam dokumen kekuatan hukum, khususnya dokumen yang berkaitan dengan kontrak yang terjadi di masyarakat. Dengan perkembangan hubungan antara subjek hukum warga negara yang berbeda untuk membuat perjanjian dalam bahasa asing selalu digunakan antara pihak-pihak yang memilikin perbedaan karena perbedaan bahasa kewarganegaraan.DOI:10.15408/jch.v1i1.2922

  17. Penerapan Algoritma Squeezer untuk Memberikan Rekomendasi Pilihan Lagu Berdasarkan Daftar Lagu yang Dimainkan pada Pemutar Mp3 Android

    Directory of Open Access Journals (Sweden)

    Eko Wahyu Wibowo

    2013-03-01

    Full Text Available Pada tulisan ini dibuat sebuah aplikasi pemutar mp3 pada perangkat Android dan juga layanan web yang terintegrasi menjadi sebuah sistem yang dapat memberikan rekomendasi lagu kepada penggunanya. Rekomendasi yang diberikan akan didasarkan pada  riwayat lagu yang sering didengarkan oleh pengguna. Untuk menghasilkan rekomendasi digunakan algoritma Squeezer. Algoritma Squeezer membantu dalam mengelompokkan riwayat lagu pengguna dan memberikan rekomendasi lagu baru yang mirip dengan kelompok riwayat lagu yang paling besar. Berdasarkan hasil pengujian yang dilakukan tingkat akurasi rekomendasi yang diberikan mencapai 80% serta mendapat respon yang baik dari pengguna.

  18. PERFORMA FOTOSINTESIS Kappaphycus sp. (strain Sumba) YANG DIUKUR BERDASARKAN EVOLUSI OKSIGEN TERLARUT PADA BEBERAPA TINGKAT SUHU DAN CAHAYA

    OpenAIRE

    Lideman Lideman; Asda Laining

    2015-01-01

    Penelitian ini bertujuan untuk mengetahui pengaruh suhu dan cahaya terhadap laju fotosintesis Kappaphycus sp. (strain Sumba) yang diukur berdasarkan perubahan oksigen terlarut. Pengukuran laju fotosintesis Kappaphycus sp. pertama-tama dilakukan pada suhu 20oC, 24oC, 28oC, dan 32oC pada tingkat cahaya 353 μmol photons m-2 s-1 untuk mendapatkan kurva fotosintesis versus suhu (kurva P-T). Selanjutnya, pengukuran laju fotosintesis dilakukan pada suhu 20oC, 24oC, dan 28oC dengan intensitas cahaya ...

  19. The ATM and Rad3-Related (ATR) Protein Kinase Pathway Is Activated by Herpes Simplex Virus 1 and Required for Efficient Viral Replication.

    Science.gov (United States)

    Edwards, Terri G; Bloom, David C; Fisher, Chris

    2018-03-15

    The ATM and Rad3-related (ATR) protein kinase and its downstream effector Chk1 are key sensors and organizers of the DNA damage response (DDR) to a variety of insults. Previous studies of herpes simplex virus 1 (HSV-1) showed no evidence for activation of the ATR pathway. Here we demonstrate that both Chk1 and ATR were phosphorylated by 3 h postinfection (h.p.i.). Activation of ATR and Chk1 was observed using 4 different HSV-1 strains in multiple cell types, while a specific ATR inhibitor blocked activation. Mechanistic studies point to early viral gene expression as a key trigger for ATR activation. Both pATR and pChk1 localized to the nucleus within viral replication centers, or associated with their periphery, by 3 h.p.i. Significant levels of pATR and pChk1 were also detected in the cytoplasm, where they colocalized with ICP4 and ICP0. Proximity ligation assays confirmed that pATR and pChk1 were closely and specifically associated with ICP4 and ICP0 in both the nucleus and cytoplasm by 3 h.p.i., but not with ICP8 or ICP27, presumably in a multiprotein complex. Chemically distinct ATR and Chk1 inhibitors blocked HSV-1 replication and infectious virion production, while inhibitors of ATM, Chk2, and DNA-dependent protein kinase (DNA-PK) did not. Together our data show that HSV-1 activates the ATR pathway at early stages of infection and that ATR and Chk1 kinase activities play important roles in HSV-1 replication fitness. These findings indicate that the ATR pathway may provide insight for therapeutic approaches. IMPORTANCE Viruses have evolved complex associations with cellular DNA damage response (DDR) pathways, which sense troublesome DNA structures formed during infection. The first evidence for activation of the ATR pathway by HSV-1 is presented. ATR is activated, and its downstream target Chk1 is robustly phosphorylated, during early stages of infection. Both activated proteins are found in the nucleus associated with viral replication compartments and in

  20. Analisis Kemampuan Komunikasi Matematis Melalui Pembelajaran Model 4K Berdasarkan Tipe Kepribadian Peserta Didik Kelas VII

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    Ajeng Dian Pertiwi

    2014-11-01

    Full Text Available Penelitian ini bertujuan untuk mendeskripsikan kemampuan komunikasi matematis  melalui pembelajaran model 4K berdasarkan tipe kepribadian Guardian, Artisan, Rational, dan Idealist. Subjek penelitian dalam penelitian ini terdiri dari 4 peserta didik kelas VII G SMP Negeri 2 Semarang. Teknik pengumpulan data penelitian ini adalah tes komunikasi matematis dan wawancara. Hasil tes dan wawancara dianalisis mengacu pada kriteria kemampuan komunikasi matematis yakni kemampuan: (1 menuliskan apa yang diketahui dan ditanyakan sesuai permasalahan (KKM 1; (2 menuliskan jawaban sesuai dengan maksud soal (KKM 2; (3 menuliskan alasan-alasan dalam menjawab soal (KKM 3; (4 membuat gambar yang relevan dengan soal (KKM 4; (5 menuliskan istilah-istilah dan simbol-simbol matematika (KKM 5; dan (6 membuat simpulan secara tertulis menggunakan bahasa sendiri. (KKM 6  Hasil penelitian menunjukkan bahwa: (1 Guardian menguasai KKM 1, 2, 3, 4, dan 5, namun kurang menguasai KKM 6; (2 Artisan menguasai KKM 1, 2, 3, dan  4, namun kurang menguasai KKM 5 dan 6; (3 Rational menguasai keenam KKM; (4 Idealist menguasai KKM 1, 2, dan 4, namun kurang menguasai KKM 3, 5, dan 6.The purpose of this research is obtain a description of mathematical communication through 4K learning model based on personality types Guardian, Artisan, Rational, and Idealist. The subjects of this research consist of 4 students 7th grade in state junior high school 2 Semarang. The techniques to collect data of this research are mathematical communication test and interviews. Test result and interviews are analyzed based on the mathematical communication ability criteria, they are the ability of: (1 writing what are known and what are asked (MCA1; (2 writing an answer appropriate with the problem intention (MCA 2; (3 writing the reason in problem solving (MCA 3; (4 making a sketch related to problem (MCA 4; (5 writing the technical terms and mathematics symbols (MCA 5; and (6 writing a conclusion with own

  1. Pencarian Ruang Warna Kulit Manusia Berdasarkan Nilai Karakteristik (λ Matrik Window Citra

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    Putra Pandu Adikara

    2014-07-01

    Full Text Available Abstrak Perkembangan transaksi dan distribusi data yang sangat besar, terutama saat teknologi informasi dan komunikasi melalui  web bisa dijangkau oleh siapa saja menggunakan perangkat yang semakin beragam, membuat pengguna memerlukan aplikasi yang serba mudah untuk digunakan. Diantaranya adalah identifikasi obyek yang berada dalam data multimedia berupa teks, gambar maupun suara. Deteksi warna, terutama deteksi warna kulit manusia adalah tahap awal identifikasi keberadaan manusia pada citra 2 dimensi. Terdapat sejumlah metode untuk menentukan apakah suatu pixel pada gambar tersebut merupakan warna kulit manusia. Penelitian sebelumnya telah membuat ruang warna berbasis pixel diantaranya adalah ruang warna RGB, normalisasi RGB, HIS/HSV, TSL, YCbCr dll. Suatu matrik bujur sangkar NxN mempunyai nilai karakteristik (λ sebanyak N dimana nilai masing-masing berupa bilangan real. Suatu citra dapat dipecah menjadi M matrik bujur sangkar dan kemudian dicari nilai λ  nya. Penelitian ini akan mencari ruang warna kulit manusia berdasarkan nilai karakteristik (ƛ matrik window citra. Dari hasil pengujian hamper semua warna kulit dapat dideteksi, namun image untuk warna kulit yang tidak mencolok beberapa obyek pada image dapat ditampilkan dengan baik meskipun bukan kulit. Kata kunci: Citra Kulit, Nilai Karakteristik (λ, Matrik Window Abstract The development of the transaction and distribution of huge data, especially when the information technology and communication via the web can be reached by anyone using the increasingly diverse, making the user requires an application that completely easy to use. Among them is the identification of objects that are in the multimedia data such as text, images and sound. Color detection, particularly the detection of human skin color is an early stage identification of human presence on the 2-dimensional image. There are a number of methods to determine whether a pixel in the image is the color of human skin

  2. Remedial Teaching untuk Mengatasi Kesulitan Belajar Siswa pada Kemampuan Pemecahan Masalah Matematika berdasarkan Prosedur Newman

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    Hasan Hafid

    2017-03-01

    Full Text Available Penelitian ini dilatarbelakangi oleh temuan kesulitan belajar siswa dalam belajar matematika. Tujuan penelitian ini adalah untuk mendiagnosis kesulitan belajar siswa dalam kemampuan pemecahan masalah berdasarkan prosedur Newman dan melaksanakan remedial teaching untuk mengatasi kesulitan belajar siswa. Pembelajaran kooperatif tipe Think Pair Share diajarkan kepada siswa untuk memberikan kemampuan pemecahan masalah. Subjek penelitian adalah sembilan siswa kelas X Mipa 1 di SMA Negeri 1 Petarukan yang diambil 3 siswa pada kelompok atas, tengah, dan bawah. Hasil penelitian ini adalah: 1 kebanyakan letak kesulitan belajar siswa adalah pada tahap memahami (comprehension dan tranformasi. Untuk kesalahan process skill dan encoding terjadi karena siswa mengalami kesalahan pada tahap sebelumnya. Temuan faktor penyebab kesulitan belajar siswa yaitu 3 siswa mengalami kesulitan belajar bersifat fisiologis, 5 siswa bersifat  psikologis,  1  siswa  bersifat  pedagogis,  6  siswa  bersifat  sosiologis;  2 remedial teaching berhasil mengatasi kesulitan belajar sebanyak 8 dari 9 siswa atau 89% siswa. Sehingga dapat dikatakan remedial teaching efektif  dalam mengatasi kesulitan belajar. This research was motivated by findings of students' learning difficulties in learning mathematics. The purpose of this research was to diagnose students' learning difficulties in problem  solving  skill  based  Newman  procedures  and  implement  remedial  teaching  for eclipse students learning difficulties. Cooperative learning Think Pair Share was taught to students to give problem­solving skills. The subjects were nine students class of  X science 1 at SHS 1 Petarukan taken three students in the top group, middle, and bottom. The results of  this research are: 1 the most mistake of  student learning difficulty was at the stage of understanding (comprehension and transformation. For process skill and encoding errors occur because students had an error

  3. Analisis Perbandingan Kinerja Mach-Zehnder berdasarkan Ragam Format Modulasi pada Jaringan FTTH

    Directory of Open Access Journals (Sweden)

    ZULIA NURUL KARIMAH

    2018-03-01

    Full Text Available ABSTRAK Pada jurnal ini dibuat pemodelan link FTTH pada software Optisystem 7.0 untuk mengetahui pengaruh dari Kerr effect dengan membandingkan performansi serat optik kaca dan serat optik plastik berdasarkan format modulasi berupa NRZ, RZ, RZ-DPSK, RZ-DQPSK dan CSRZ. Terdapat dua skenario, dengan skenario pertama, variabel input yang diubah adalah format modulasi pada Mach-zehnder, sedangkan pada skenario kedua, variabel yang diubah adalah pemakaian serat optik yang dipakai, yaitu serat optik bahan kaca, plastik dan hybrid kaca plastik. Hasil simulasi menunjukkan dengan efek linier dan non-linier pada kabel kaca yang menghasilkan performansi jaringan dari yang terbaik, dengan Q factor di atas 6 dan BER di bawah 10-9 adalah NRZ, RZ, RZ-DPSK, CSRZ dan RZ-DQPSK. Sedangkan dengan penggunaan kabel PMMA, yang menunjukkan performansi jaringan yang baik adalah dengan konfigurasi G652D-G652D-PMMA pada format modulasi NRZ, RZ, RZ-DPSK dan RZ-DQPSK. Efek non-linier yang terjadi pada jaringan ini hanya SPM dan XPM. Kata kunci: FTTH, mach-zehnder, format modulasi, efek non-linier, GOF, POF. ABSTRACT In this journal is creating a FTTH link on Optisystem software 7.0 to determine the effect of Kerr effect by comparing the performance of fiber optic glass and plastic optical fiber based on modulation formats such as NRZ, RZ, RZ-DPSK, RZ-DQPSK and CSRZ. There are two scenarios, first, input variables are changed based on format in Mach-zehnder modulator, while in the second scenario, the changed variable is the material of optical fiber, the materials are optical fiber glass, plastic and hybrid plastic and glass. The simulation results based on comparison with linear and nonlinear effects on glass optical fiber, which produce Q factor above 6 and BER below 10-9 are NRZ, RZ, RZ-DPSK, CSRZ and RZ-DQPSK. While the use of PMMA cable, which indicates good network performance is the configuration G652D-G652D-PMMA on the modulation format NRZ, RZ, RZ-DPSK and RZ

  4. Analisis Kepuasan Pelanggan Pembuatan Sertifikat Tanah Dengan Menggunakan Servqual Dan Kansei Engineering Berdasarkan Twitter BPN Salatiga

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    Juwita Artanti Kusumaningtyas

    2017-08-01

    Full Text Available Abstrak BPN (Badan Pertanahan Nasional merupakan instansi pemerintah dengan tugas di bidang pertanahan sesuai dengan Peraturan Presiden Republik Indonesia Nomor 20 Tahun 2015. Salah satu tugas BPN yaitu melaksanakan kebijakan penetapan hak tanah, pendaftaran tanah, dan pemberdayaan masyarakat. Fungsi tugas pendaftaran dan penetapan hak tanah tertuang dalam salah satunya pelayanan BPN yaitu pembuatan sertifikat tanah. Pemberdayaan masyarakat sendiri dilakukan BPN Salatiga dengan memanfaatkan media sosial twitter untuk berbagi informasi kepada masyarakat terkait program kerja dan pelayanan. Melalui twitter BPN Salatiga, masyarakat dapat mengetahui pelayanan BPN Salatiga dan menjadi forum diskusi masyakarat dengan pihak BPN, sehingga dapat mengetahui keluhan dan harapan masyarakat terhadap pelayanan BPN Salatiga khususnya mengenai sertifikat tanah. Berdasarkan tweet BPN Salatiga ada 20 atribut layanan yang akan diolah dengan metode Servqual dan menggunakan metode Kansei Engineering untuk melakukan perbaikan. Analisis ini diharapkan dapat memberi pandangan kepada BPN untuk mengetahui kualitas pelayanan yang diberikan kepada pelanggan dan mengetahui kepuasan pelanggan terhadap pelayanan sertifikat tanah melalui twitter BPN Salatiga. Hasilnya tingkat harapan tertinggi ada pada dimensi Tangible (4,50 dan persepsi tertinggi pada dimensi Reliability (4,49 dengan 12 kansei words. Kata kunci: Kepuasan Pelanggan, Servqual, Kansei Engineering, Twitter Abstract BPN (National Land Agency is a government agency with a task in the field of land in accordance with the Presidential Regulation of the Republic of Indonesia No. 20 of 2015. One of the duties of BPN is implementing the policy of land titling, land registration, and community empowerment. The function of registration and assignment of land rights is contained in one of the BPN services namely the making of land certificate. Community empowerment itself is done by BPN Salatiga by utilizing social media

  5. ANALISIS PENERIMAAN PENGGUNAAN OTOMASI PERPUSTAKAAN UNNES BERDASARKAN PENDEKATAN TECHNOLOGY ACCEPTANCE MODEL (TAM

    Directory of Open Access Journals (Sweden)

    Mia Winda Anzaeni

    2017-06-01

    Full Text Available Otomasi Perpustakaan merupakan sistem informasi perpustakaan berbasis web di Universitas Negeri Semarang. Penelitian ini bertujuan untuk mengetahui pengaruh dan menganalisis faktor-faktor yang mempengaruhi penerimaan penggunaan Otomasi Perpustakaan. Pendekatan yang digunakan adalah Technology Acceotance Model (TAM. Populasi dalam penelitian ini adalah mahasiswa pendidikan akuntansi di Universitas Negeri Semarang. Jumlah sampel sebanyak 127 responden. Teknik pengambilan sampel menggunakan non probablity sampling dengan sampling kuota. Metode pengumpulan data menggunakan kuesiioner. Data dianalisis menggunakan teknik path analysis. Hasil penelitian menunjukkan bahwa ada pengaruh positif dan signifikan manfaat (PU terhadap penerimaan (ACC sebesar 24,5%, kemudahan (PEOU terhadap ACC sebesar 24,8%, ATU terhadap ACC sebesar 40,7%, PU terhadap ATU sebesar 25,1%, PEOU terhadap ATU sebesar 24,5%, PU melalui ATU terhadap ACC sebesar 10,2% dan PEOU melalui ATU terhadap ACC sebesar 10%. Berdasarkan penelitian, dapat disimpulkan bahwa terdapat pengaruh perceived usefulness dan perceived ease of use terhadap acceptance baik secara langsung maupun tidak langsung dan pengarung langsung attitude terhadap acceptance. Disarankan kepada pengelola website Otomasi Perpustakaan agar dapat meningkatkan fungsi dari web sehingga pengguna dapat merasakan manfaat dan kemudahan web. Disarankan juga kepada peneliti selanjutnya untuk meneliti dengan objek yang berbeda, serta menambahkan variabel eksternal yang sesuai dengan penelitiannya. Library Automation is a web-based library information systems at the State University of Semarang. This study aims to determine the effect and analyze the factors that affect the acceptance of the use of Library Automation. The population in this research were students of accounting education at State University of Semarang. The total sample of 127 respondents. The sampling technique used is non-probability sampling with quota sampling

  6. Analisis Perbandingan Kinerja Mach-Zehnder berdasarkan Ragam Format Modulasi pada Jaringan FTTH

    Directory of Open Access Journals (Sweden)

    ZULIA NURUL KARIMAH

    2017-06-01

    Full Text Available ABSTRAKPada jurnal ini dibuat pemodelan link FTTH pada software Optisystem 7.0 untuk mengetahui pengaruh dari Kerr effect dengan membandingkan performansi serat optik kaca dan serat optik plastik berdasarkan format modulasi berupa NRZ, RZ, RZ-DPSK, RZ-DQPSK dan CSRZ. Terdapat dua skenario, dengan skenario pertama, variabel input yang diubah adalah format modulasi pada Mach-zehnder, sedangkan pada skenario kedua, variabel yang diubah adalah pemakaian serat optik yang dipakai, yaitu serat optik bahan kaca, plastik dan hybrid kaca plastik. Hasil simulasi menunjukkan dengan efek linier dan non-linier pada kabel kaca yang menghasilkan performansi jaringan dari yang terbaik, dengan Q factor di atas 6 dan BER di bawah 10-9 adalah NRZ, RZ, RZ-DPSK, CSRZ dan RZ-DQPSK. Sedangkan dengan penggunaan kabel PMMA, yang menunjukkan performansi jaringan yang baik adalah dengan konfigurasi G652D-G652D-PMMA pada format modulasi NRZ, RZ, RZ-DPSK dan RZ-DQPSK. Efek non-linier yang terjadi pada jaringan ini hanya SPM dan XPM.Kata kunci: FTTH, mach-zehnder, format modulasi, efek non-linier, GOF, POF.ABSTRACTIn this journal is creating a FTTH link on Optisystem software 7.0 to determine the effect of Kerr effect by comparing the performance of fiber optic glass and plastic optical fiber based on modulation formats such as NRZ, RZ, RZ-DPSK, RZ-DQPSK and CSRZ. There are two scenarios, first, input variables are changed based on format in Mach-zehnder modulator, while in the second scenario, the changed variable is the material of optical fiber, the materials are optical fiber glass, plastic and hybrid plastic and glass. The simulation results based on comparison with linear and nonlinear effects on glass optical fiber, which produce Q factor above 6 and BER below 10-9 are NRZ, RZ, RZ-DPSK, CSRZ and RZ-DQPSK. While the use of PMMA cable, which indicates good network performance is the configuration G652D-G652D-PMMA on the modulation format NRZ, RZ, RZ-DPSK and RZ

  7. STUDI RESPON SISWA DALAM MENYELESAIKAN SOAL PEMECAHAN MASALAH MATEMATIKA BERDASARKAN TAKSONOMI SOLO

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    Rosyida Ekawati

    2013-12-01

    Full Text Available Salah satu kerangka yang digunakan sebagai rujukan menganalisis respon siswa yaitu taksonomi SOLO. Tujuan penelitian adalah (1 menemukan pola  tingkat respon siswa dalam menyelesaikan soal pemecahan masalah berdasarkan taksonomi SOLO dan (2 menemukan hambatan siswa dalam menyelesaikan soal pemecahan masalah matematika. Metode penelitian ini yaitu pendekatan kualitatif. Sumber data penelitian  ini adalah 24 siswa putri dan 18 siswa putra kelas VIII SMP IT Darul Fikri Bawen. Hasil penelitian ini adalah respon siswa putri pada level prestructural sebanyak 25, 42%, unistructural sebanyak 10, 83%, multistructural sebanyak   32, 92%, relational sebanyak 20, 83% dan extended abstract sebanyak 10%. Untuk hasil respon siswa putra pada level prestructural sebanyak 16, 67%, unistructural sebanyak 9, 44%, multistructural sebanyak 32, 22%, relational sebanyak 38, 33% dan extended abstract sebanyak 3, 33 %.  Rekomendasi dari penelitian ini yaitu  guru perlu memberi banyak latihan keterampilan memahami masalah, mengaplikasikan rumus, dan meninjau kembali jawaban.One of frameworks used as a reference to analyze students’ responses is SOLO taxonomy. The purposes of the study were to find out the pattern of students’ responses levels in doing problem solving items based on SOLO taxonomy and to find out the students’ obstacles in doing mathematical problem solving items. The research method used in this study was a qualitative research. The data sources of this study were 24 female students and 18 male students of the eight grade of SMP IT Darul Fikri Bawen. The results of the study showed that for female students, 25,42% of them belonged to prestructural level, 10,83% of them belonged to unistructural response level, 32,92 % of them belonged to multistructural response level, 20,83% of them belonged to relational response level, and 10% of them belonged to extended abstract response level. Meanwhile, for male students, 16,67% of them belonged to

  8. STRATEGI DAN PROSES BERPIKIR DALAM MENYELESAIKAN SOAL PEMECAHAN MASALAH BERDASARKAN TINGKAT KECEMASAN MATEMATIKA

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    Nurul Ismawati

    2015-03-01

    Full Text Available Strategi dan proses berpikir memiliki peran sangat penting dalam proses pemecahan masalah. Penelitian ini bertujuan untuk memperoleh deskripsi tentang strategi dan proses berpikir dalam menyelesaikan soal pemecahan masalah siswa kelas VII dengan tingkat kecemasan matematika tinggi, sedang, maupun rendah. Penelitian ini merupakan penelitian deskriptif dengan pendekatan kualitatif. Subyek dalam penelitian ini adalah 3 siswa dengan kecemasan matematika tinggi (KMT, 3 siswa dengan kecemasan matematika sedang (KMS, dan 3 siswa dengan kecemasan matematika rendah (KMR kelas VII MTs NU Nurul Huda Kudus. Penetapan subyek berdasarkan hasil tes skala kecemasan matematika. Hasil penelitian menunjukkan bahwa strategi dan proses berpikir diketahui sebagai berikut (1 tiga subyek KMT tidak dapat menggunakan sebagian besar tahapan strategi dan proses berpikir dalam menyelesaikan soal pemecahan masalah dengan tepat sehingga jawaban tidak tepat, (2 tiga subyek KMS dapat menggunakan sebagian besar tahapan strategi dan proses berpikir dalam menyelesaikan soal pemecahan masalah dengan tepat terhadap beberapa soal yang diberikan, dan (3 tiga subyek KMR dapat menggunakan sebagian besar tahapan strategi dan proses berpikir dalam menyelesaikan soal pemecahan masalah dengan tepat dan memperoleh jawaban tepat.Strategy and thinking process have a very important role in the process of problem solving. This study aimed to obtain a description of the strategies and thought processes in solving problem solving seventh grade students with mathematics anxiety levels. This research is a descriptive qualitative approach. Subjects in this study were three students with high math anxiety (KMT, 3 students with math anxiety medium (KMS, and 3 students with low math anxiety (KMR class VII MTs. NU NU Nurul Huda. Determination of the subject based on the results of tests of mathematics anxiety scale. The results showed that the strategies and thought processes KMT three subjects can not

  9. Konsep Persatuan Dengan Kematian Dan Kebangkitan Kristus Berdasarkan Roma 6:1-14

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    Hanny Frederik

    2015-09-01

    Full Text Available Persatuan orang percaya dengan Kristus adalah doktrin yang merupakan pemikiran kunci dalam ajaran Tuhan bahkan sangat penting bagi teologi Paulus sehingga seorang penafsir, James S. Stewart menyebutnya sebagai “inti dari agama Paulus.” Menurut hasil uraian tentang konsep persatuan dengan kematian dan kebangkitan Kristus berdasarkan Roma 6:1-14, maka penulis menarik beberapa kesimpulan sebagai berikut: Pertama, orang percaya telah dipersatukan dengan kematian dan kebangkitan Kristus melalui baptisan, yang berarti ia turut serta mengalami peristiwa-peristiwa yang dialami oleh Kristus dalam sejarah, yakni penyaliban, kematian, penguburan dan kebangkitan Kristus. Kedua, persatuan dengan kematian dan kebangkitan Kristus mengakibatkan berlalunya ciptaan lama, yaitu kematian manusia lama sebagai status atau kedudukan seseorang dalam persekutuannya dengan Adam. Kematian Kristus adalah kematian bagi dosa, karena itu dalam persatuan dengan kematian Kristus, orang percaya juga telah mati bagi dosa. Ketiga, persatuan dengan kematian dan kebangkitan Kristus menghasilkan ciptaan baru, yaitu kehidupan baru sebagai status atau kedudukan orang percaya dalam persekutuan dengan Kristus. Keempat, kehidupan yang berpadanan dengan status baru orang percaya dalam Kristus adalah kehidupan dalam pengudusan yang meliputi hidup dalam pertobatan dan hidup untuk melayani Allah.The unity of the believer with Christ is a doctrine which is a key thought in the teaching of Jesus, moreover it is very important for Paul’s theology such that one commentator, James S. Stewart, delineates it as the “Man in Christ: The Vital Elements of St. Paul’s Religion.” Based upon the results of an analysis about the concept of unity in the death and resurrection of Christ based upon Romans 6: 1-14, the author draws several conclusions as follows: First, believers have been made one with the death and resurrection of Christ through baptism, which means the believer participates in

  10. KETERKAITAN KETAHANAN PANGAN DENGAN KEMISKINAN BERDASARKAN IMPLEMENTASI KEBIJAKAN PENANGGULANGAN KEMISKINAN DI INDONESIA

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    Suprianto Damanik

    2017-06-01

    Full Text Available Jenis penelitian ini adalah kuantitatif dengan data time series  berdasarkan tahun periode 1983-2013. Data diperoleh dari Badan Pusat Statistik, Litbang, Bulog dan world bank. Metode analisis yang digunakan adalah Regresi linier berganda dan Granger casuality. Pengujian untuk melihat pengaruh antar variabel dan menguji keterkaitan antara ketahanan pangan dengan kemiskinan. Hasil penelitian regresi linier berganda menunjukkan bahwa luas panen berpengaruh positif dan signifikan terhadap ketahanan pangan nasional yaitu sebesar 0,0357, harga beras berpengaruh negatif dan signifikan terhadap ketahanan pangan nasional yakni sebesar 0,0020, Impor beras berpengaruh negatif dan tidak signifikan terhadap ketahanan pangan nasional yakni sebesar 0,0725. Dan hasil uji granger causality menunjukkan bahwa tidak adanya hubungan kausal antara ketahanan pangan dengan kemiskinan di Indonesia. Kesimpulan dari penelitian ini adalah Luas panen padi berpengaruh positif dan signifikan terhadap ketahanan pangan nasional di Indonesia, harga beras berpengaruh negatif dan signifikan terhadap ketahanan pangan nasional di Indonesia, Impor beras berpengaruh negative dan tidak signifikan terhadap ketahanan pangan nasional di Indonesia, dan tidak terjadi hubungan casusal antara ketahanan pangan dengan kemiskinan di Indonesia.  This research is a quantitative time series data based on the period 1983-2013. Data obtained from the Central Bureau of Statistics, Research and Development, Bulog and the World Bank. The analytical method used is multiple linear regression and Granger casuality. Testing to see the influence between variables and examine the linkages between food security and poverty. Multiple linear regression results indicate that the area harvested positive and significant impact on national food security is equal to 0.0357, the price of rice a significant negative effect on the national food security which is equal to 0.0020, imports of rice and no significant

  11. PEMETAAN MODEL KERAWANAN LEPTOSPIROSIS BERDASARKAN FAKTOR RISIKO LINGKUNGAN DAN TRAP SUCCESS DI BANTUL, YOGYAKARTA

    Directory of Open Access Journals (Sweden)

    Sunaryo Sunaryo

    2016-09-01

    Full Text Available ABSTRACTLeptospirosis is zoonotic disease, which is caused by leptospira bacteria and transmitted to human bycontact with contaminated animal urine. Bantul District is endemic area of leptospirosis, case fatality rateas much 11% in 2010. The aim of this research was to study distribution of spatial epidemiologyleptospirosis, and mapping of vulnerable leptospirosis model by using Geographical Information Systembased on environmental risk factor and trap success in Bantul.This research done in March until November2011, and applied spatial analysis by using cross sectional design. Result of this research• showedleptospirosis cases in 2011 reaching 135 cases, and group of adult men was dominant, mostly as farmer andveteriner worker. There were three claster with average distance between cases: 0 km and furthermost 30km. Leptospirosis pattern increased in March and April when high rainfall. Medium rainfall spatial,lowland, vegetation index medium, alluvial type of soil, existence of rat and landuse of ricefield wereenvironmental variable influence leptospirosis cases. High vulnerable leptospirosis zone located in Centerand West Bantul.Keyword : Mapping, leptospirosis, vulnerable model. ABSTRAK Leptospirosis merupakan penyakit bersumber rodensia, disebabkan oleh bakteri leptospira, menular melalui kulit luka atau mukosa dengan air/tanah tercemar leptospira dariurine binatang. Kabupaten Bantul merupakan daerah endemis leptospirosis,case fatality rate mencapai11% pada tahun2010. Penelitian ini bertujuan ingin mengetahui persebaran epidemiologi spasial leptospirosis, dan pemetaanmodel daerah rawan Leptospirosisdi Kabupaten Bantul berdasarkan faktor risiko lingkungan dan trap success tikus dengan aplikasi Sistem Informasi Geografis. Penelitian ini dilakukanpada Bulan Maret sampaiNovember 2011, menggunakan metode analisis spasial deskriptif dengan pendekatan potong lintang. Hasil: Kasus leptospirosispada tahun2011 mencapai135 kasusyang didominasi oleh

  12. Proses Berpikir Siswa SD dalam Melakukan Estimasi Masalah Berhitung Berdasarkan Jenis Kelamin

    Directory of Open Access Journals (Sweden)

    Muh. Rizal

    2013-01-01

    Full Text Available Abstract: The Thinking Process of Primary-school Students of Different Sexes in Estimating Arithmetic Problems. This case study is intended to explore the thinking process of the fifth-year students of different sexes in estimating arithmetic problems. Two students, one male and one female, belonging to the high achievers in a mathematics test were selected for the study and then interviewed, assigned to solve arithmetic problems, and finally asked to think aloud their thinking process. The study reveals that the thinking process of both the male and female subjects is in the form of accommodation as they ap­proached the arithmetic problems by repeatedly reading the tasks. Though employing the same thinking process, in the planning stage, the male subject made use of rounding and compatible number strategies, whereas the female one used only rounding strategy. In implementing the plan, the male subject em­ployed mental counting through assimilation, but the female one used algorithm through accommoda­tion. In the evaluating stage, the male subject traced back his work through mental counting, while the female one utilized reverse operations. Abstrak: Proses Berpikir Siswa SD dalam Melakukan Estimasi Masalah Berhitung Berdasarkan Jenis Kelamin. Penelitian ini ingin mendeskripsikan proses berpikir siswa laki-laki dan perempuan yang berkemampuan matematika tinggi dalam melakukan estimasi. Penelitian dilakukan di kelas V SD, dengan subjek satu siswa laki-laki dan satu siswa perempuan yang memiliki skor 75 ke atas dalam tes kemampuan matematika. Data dikumpulkan dengan wawancara, pemberian tes dan think aloud. Analisis dilakukan dengan menelaah seluruh data, reduksi data, pengolompokan data, kategorisasi, pengkodean, dan pemeriksaan kredibilitas data dengan triangulasi waktu. Hasil penelitian menunjukkan bahwa proses berpikir keduanya adalah akomodasi. Dalam membuat rencana, laki-laki menggunakan rounding dan compatible number strategy, sedangkan

  13. IMPLEMENTASI PENGISIAN JABATAN KEPALA DAN WAKIL KEPALA DAERAH BERDASARKAN UUD 1945 DI TENGAH KEBERAGAMAN DAERAH

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    Ridwan Mukti

    2016-01-01

    Full Text Available The amandement of Article 18 UUD 1945, has brought significant changes in the regional administration. One of the changes associated with  the position of head region, which previously  for this position and for the vice of head region  elected by the Legislative Council, Now it is elected trough election.  Article 18 paragraph (4 UUD 1945 states as follows : " Governor, Regent, and Mayor respectively as head of the provincial government, district, and city elected democratically. " Term " democratically elected " under Article 18 paragraph (4 UUD 1945 is implemented through the provisions of Article 56 paragraph (1 of Law No. 32 of 2004 on Regional Government, which stipulates that all regional heads both governors, regents and mayors elected as a pair of candidates directly by the people. In fact, the provisions of the law apply to all areas without considering the fact different areas , so it has attracted a wide range of issues. Though the constitution does not definitively establish that such electoral procedures. Similarly uniformity to diversity in terms of uniformity of areas including recruitment system or filling the post of regional head felt at odds with the fact of diversity in Indonesia. Keywords : Regional Head; Regional Diversity ABSTRAK Adanya perubahan ketentuan Pasal 18 UUD 1945, telah membawa perubahan yang cukup besar pada penyelenggaraan pemerintahan daerah. Salah satu perubahan itu terkait dengan pengisian jabatan kepala daerah, yang sebelumnya kepala daerah dan wakil kepala daerah dipilih oleh Dewan Perwakilan Rakyat Daerah, saat ini telah diubah dengan cara pemilihan secara demokratis. Pasal 18 ayat (4 UUD 1945 menyatakan sebagai berikut : “Gubernur, Bupati, dan Walikota masing-masing sebagai kepala pemerintah daerah provinsi, kabupaten, dan kota dipilih secara demokratis.“ Istilah  “dipilih secara demokratis “ berdasarkan Pasal 18 ayat (4 UUD 1945 tersebut  diimplementasikan melalui ketentuan Pasal 56 ayat

  14. ATR- and ATM-Mediated DNA Damage Response Is Dependent on Excision Repair Assembly during G1 but Not in S Phase of Cell Cycle.

    Science.gov (United States)

    Ray, Alo; Blevins, Chessica; Wani, Gulzar; Wani, Altaf A

    2016-01-01

    Cell cycle checkpoint is mediated by ATR and ATM kinases, as a prompt early response to a variety of DNA insults, and culminates in a highly orchestrated signal transduction cascade. Previously, we defined the regulatory role of nucleotide excision repair (NER) factors, DDB2 and XPC, in checkpoint and ATR/ATM-dependent repair pathway via ATR and ATM phosphorylation and recruitment to ultraviolet radiation (UVR)-induced damage sites. Here, we have dissected the molecular mechanisms of DDB2- and XPC- mediated regulation of ATR and ATM recruitment and activation upon UVR exposures. We show that the ATR and ATM activation and accumulation to UVR-induced damage not only depends on DDB2 and XPC, but also on the NER protein XPA, suggesting that the assembly of an active NER complex is essential for ATR and ATM recruitment. ATR and ATM localization and H2AX phosphorylation at the lesion sites occur as early as ten minutes in asynchronous as well as G1 arrested cells, showing that repair and checkpoint-mediated by ATR and ATM starts early upon UV irradiation. Moreover, our results demonstrated that ATR and ATM recruitment and H2AX phosphorylation are dependent on NER proteins in G1 phase, but not in S phase. We reasoned that in G1 the UVR-induced ssDNA gaps or processed ssDNA, and the bound NER complex promote ATR and ATM recruitment. In S phase, when the UV lesions result in stalled replication forks with long single-stranded DNA, ATR and ATM recruitment to these sites is regulated by different sets of proteins. Taken together, these results provide evidence that UVR-induced ATR and ATM recruitment and activation differ in G1 and S phases due to the existence of distinct types of DNA lesions, which promote assembly of different proteins involved in the process of DNA repair and checkpoint activation.

  15. Analisis Kesalahan Siswa Dalam Pemecahan Masalah Matematika Pada Materi Peluang Berdasarkan High Order Thinking Dan Pemberian Scaffolding

    Directory of Open Access Journals (Sweden)

    Komarudin Komarudin

    2017-09-01

    Full Text Available Dalam penelitian ini penulis menganalisis tentang kesulitan siswa dalam proses pemecahan masalah. Berdasarkan analisis, kesalahan yang dilakukan oleh siswa dalam mengerjakan soal pemecahan masalah matematika materi peluang berdasarkan langkah Polya dihasilkan dalam proses memahami masalah sebesar 100%, menyusun rencana 81%, melaksanakan rencana 81% dan memeriksa kembali solusi sebesar 100%. Tipe kesalahan yang paling sering dilakukan oleh siswa dalam mengerjakan soal pemecahan masalah matematika materi peluang antara lain, dalam proses memahami masalah yaitu kesalahan dalam menentukan apa yang diketahui dan ditanyakan, dalam proses menyusun rencana yaitu tidak menuliskan langkah-langkah yang digunakan dalam menyelesaikan masalah serta siswa menuliskan langkah yang digunakan dalam menyelesaikan masalah tetapi tidak sesuai dengan permasalahan, kesalahan dalam melaksanakan rencana yaitu tidak menuliskan rumus yang digunakan, kesalahan dalam menentukan kesimpulan yaitu tidak menuliskan kesimpulan yang diberikan, kesalahan dalam memeriksa kembali solusi yaitu tidak melakukan perhitungan ketika memeriksa kembali solusi. Cara mengatasi masalah siswa dalam menyelesaikan soal peluang dengan pemberian Scaffolding yaitu memberikan lembar kerja siswa yang berisi langkah- langkah pemecahan masalah Polya serta arahan dan tanya jawab yang berkaitan dengan proses pemecahan langkah Polya. Kegiatan scaffolding yang dilakukan antara lain environmental provisions 25%, explaining 25%, restructing 17%,  reviewing 17%, developing conceptual thinking 16%. Kegiatan ini dapat meningkatkan kemampuan pemecahan masalah siswa pada materi peluang. Hal tersebut dapat diketahui dari hasil tes kedua siswa yang menunjukkan peningkatan serta respon positif siswa setelah kegiatan scaffolding. Sehingga dapat disimpulkan bahwa kegiatan scaffolding yang dilakukan dapat memberikan dampak positif kepada siswa.

  16. Pengajaran Tentang Ibadah Berdasarkan Surat Ibrani 10:19-25 Dan Implimentasinya Dalam Kehidupan Orang Percaya Pada Masa Kini

    Directory of Open Access Journals (Sweden)

    Tison Tison

    2013-04-01

    Full Text Available Sesuai dengan permasalahan yang ada, maka tujuan penulisan karya ilmiah iniadalah: Pertama, Untuk menganalisis dan menafsirkan pengajaran tentang ibadahmenurut Ibrani 10:19-25. Kedua, Untuk membahas bagaimana mengimplementasikanpengajaran tentang ibadah dalam kehidupan orang percaya pada masa kini.Dalam penulisan karya ilmiah ini, penulis menggunakan metode penelitiannaskah Alkitab yaitu: Pertama, Metode analisis kitab, dalam hal ini menggunakanmetode penelitian yang mencakup teologi eksegesis dan kajian Alkitab untukmemahami teks yang sesuai dengan konsep yang ada dalam surat Ibrani 10:19-25. Danmenggunakan metode penelitian literatur. Kedua, Komparasi, yaitu mengadakanperbandingan-perbandingan untuk melihat kesamaan atau perbedaan pengajarantentang ibadah.Berdasarkan hasil uraian penulis dalam karya ilmiah mengenai pengajarantentang ibadah berdasarkan surat Ibrani 10:19-25 dan implimentasinya dalamkehidupan orang percaya pada masa kini, maka penulis dapat menarik kesimpulansebagai berikut: Pertama, setiap orang percaya harus mengerti dasar-dasar ibadahorang percaya yaitu darah Yesus yang telah dicurahkan bagi umat manusia yang jugatelah menjadi pengganti korban persembahan untuk datang menghampiri Allah danYesus sebagai Imam Besar. Kedua, dalam ibadah setiap orang percaya harusmemiliki sikap hati yang tulus iklhas, keyakinan iman, hati yang sudah dibasuh danberpegang pada pengakuan pengharapan pada Yesus. Ketiga, dalam ibadah, setiaporang percaya harus memiliki hati yang menyembah karena penyembahan adalah halyang paling utama untuk menyatakan hormat dan tunduk kepada Allah atas karya-Nya bagi hidup orang percaya. Keempat, dalam ibadah, setiap orang percaya harushidup dalam kekudusan, karena dalam kekudusanlah orang percaya layak dihadapanAllah.

  17. Perancangan Kamus Kompetensi Manajer Biro Pengadaan dan Manajer Bidang Komersial Pada Perusahaan PT. Barata Indonesia (Persero Berdasarkan Kamus Kompetensi Spencer

    Directory of Open Access Journals (Sweden)

    Andina Primadini Isnainia

    2017-01-01

    Full Text Available Berdasarkan hasil observasi, ditemukan bahwa Biro Pengadaaan dan Bidang Komersial memiliki peranan penting dalam perusahaan yang berfungsi dalam mengatur logistik dan rantai pasok untuk meningkatkan daya saing perusahaan. Selain itu perusahaan belum memiliki kamus kompetensi karenakan baru saja melakukan restrukturisasi. Untuk meningkatkan performa dari Manajer Biro Pengadaaan dan Manajer Bidang Komersial pada perusahaan penelitian bertujuan menyusun sebuah kamus kompetensi untuk pemangku jabatan pada Manajer Biro Pengadaaan dan Manajer  Bidang  Komersial dan mengidentifikasi kompetensi untuk pemangku jabatan tersebut. Kompetensi yang dibutuhkan didapatkan dari studi literatur yang kemudian dilakukan penyesuaian melalui kuisinoer dan wawancara. Mulanya terdapat 125 kompetensi yang dibutuhkan, setelah divalidasi   ditemukan   terdapat   49   kompetensi.   Kemudian penulis  membuat  kamus  kompetensi  dengan  metode wawancara dan Focus Group Discussion (FGD dengan melihat pola Kamus Kompetensi Spencer. Langkah selanjutnya adalah mengidentifikasi tingkat kacakapan / level dari pemangku jabatan berdasarkan kamus kompetensi yang sudah dibuat yang disajikan dalam bentuk matriks Job Competency Level. Dari penelitian ini menghasilkan Kamus Kompetensi dan matriks  Job  Competency  Level untuk jabatan Manajer  Biro Pengadaaan dan Manajer Bidang Komersial PT Barata Indonesia (Persero.

  18. PROSES BERPIKIR SISWA SEKOLAH MENENGAH PERTAMA DALAM MEMECAHKAN MASALAH MATEMATIKA BERDASARKAN LANGKAH-LANGKAH POLYA DITINJAU DARI ADVERSITY QUOTIENT

    Directory of Open Access Journals (Sweden)

    Muhammad Yani

    2016-06-01

    Full Text Available Penelitian ini dilakukan untuk menjelaskan proses berpikir dan menganalisis kesulitan siswa dalam memecahkan masalah matematika berdasarkan pengukuran Polya ditinjau dari Adversity Quotient (AQ. Penelitian ini merupakan penelitian deskirptif kualitatif dengan subjek penelitian adalah siswa dari kelas IX SMP N 1 Banda Aceh tediri dari tiga siswa. Pemilihan subjek penelitian menggunakan metode purposive sampling dan berdasarkan tingkatan AQ (climber, camper, dan quitter dan komunikasi (lisan dan tertulis. Pengumpulan data menggunakan wawancara berbasis tugas, dan triangulasi untuk mengecek validitas data. Data dianalisis menggunakan konsep dari Miles dan Huberman: yaitu tahap pengurangan data, presentasi data, dan kesimpulan. Hasil menunjukkan bahwa: (1 Proses berpikir dari subjek climber yaitu secara asimilasi dalam memahami, merencanakan penyelesaian, .serta mengecek kembali; (2 Subjek camper juga berpikir secara asimilasi pada tahap memahami masalah, merencanakan penyelesaian, dan mengecek kembali; (3 subjek quitter berpikir secara akomodasi dalam memahami masalah dan menyelesaikan masalah. Kata kunci: Proses Berpikir, Pemecahan Masalah, Tahap Polya, Adversity Quotient (AQ DOI: http://dx.doi.org/10.22342/jpm.10.1.3278.42-57

  19. Analisis Zona Permeabel Fluida Sistem Panas Bumi Gunungapi Slamet Berdasarkan Analisis Kerapatan Kelurusan Citra SRTM Dan Struktur Geologi

    Directory of Open Access Journals (Sweden)

    Sachrul Iswahyudi

    2016-03-01

    Full Text Available Keberadaan manifestasi panasbumi di sekitar Gunungapi Slamet tidak dapat dipisahkan dari zona-zona permeabilitas yang berkembang. Lokasi-lokasi lulus air tersebut (zona permeabel yang memungkinkan terbentuknya sirkulasi fluida tempat air masuk untuk mengisi reservoir panas bumi dan air keluar ke permukaan bumi sebagai manifesatasi mata air panasbumi di sekitar Gunungapi Slamet. Publikasi yang berupa hasil penelitian ini mencoba mengidentifikasi zona-zona permeabel tersebut berdasarkan anaslisis kerapatan kelurusan yang terekam dalam citra SRTM. Identifikasi kelurusan-kelurusan pada citra berdasarkan komponen-komponen interpretasi citra, yaitu tona, tekstur, pola, bentuk dan relief. Hasil analisis tersebut dikompilasi dengan data struktur geologi regional yang sebelumnya telah diidentifikasi dan data lapangan berupa manifestasi mata air panas. Lokasi-lokasi dengan kerapatan kelurusan yang tinggi pada citra SRTM umumnya bersesuaian dengan zona struktur geologi regional keberadaan manifestasi mata air panas. Daerah tersebut memanjang relatif utara-selatan di bagian barat dan timur-barat di bagian selatan peta. Daerah-daerah inilah yang merupakan daerah lulus air tempat fluida bersirkulasi membentuk sistem panasbumi Gunungapi Slamet.

  20. PERANCANGAN TATA LETAK DAN FASILITAS HUNIAN RUKOST BERDASARKAN KEBUTUHAN KONSUMEN UNTUK MENINGKATKAN PANGSA PASAR DENGAN MENGGUNAKAN ASPEK ERGONOMI

    Directory of Open Access Journals (Sweden)

    Leola Dewiyani

    2018-04-01

    Full Text Available Saat ini Karawang dikenal sebagai daerah industri yang paling berpotensi di wilayah Jawa Barat. Hal tersebut dibuktikan dengan banyaknya pabrik yang didirikan di tanah Pasundan ini. Dengan banyaknya usaha baik berupa pabrik maupun yang lainnya, Karawang menjanjikan aneka macam peluang di berbagai bidang usaha yang bisa mendatangkan keuntungan. Perusahaan ini tentu membutuhkan tenaga kerja yang cukup banyak, tidak hanya buruh lebih dari itu tenaga kerja yang dibutuhkan juga berasal dari level menengah ke atas hingga staf profesional, hal ini yang menjadi daya tarik bagi pengembang untuk membangun bisnis properti di Karawang yaitu RuKost (rumah kos. Akan tetapi Tata Letak interior kamar yang ada di RuKost saat ini berdasarkan penelitian pendahuluan yang dilakukan peneliti belum memenuhi standar dan juga belum memperhatikan kenyamanan, kepuasan, keselamatan dan kesehatan karena desain layout interior yang ada pada kamar RuKost saat ini kurang memperhatikan prinsip-prinsip ergonomik. Berdasarkan kondisi di atas maka akan dilakukan suatu redesign terhadap tata letak interior kamar RuKost. Metode perancangan yang akan digunakan dalam penelitian ini adalah antropometri dan physiological performance.

  1. Cadmium depletion impacts on hardening neutron spectrum for advanced fuel testing in ATR

    International Nuclear Information System (INIS)

    Chang, Gray S.

    2011-01-01

    For transmuting long-lived isotopes contained in spent nuclear fuel into shorter-lived fission products effectively is in a fast neutron spectrum reactor. In the absence of a fast spectrum test reactor in the United States of America (USA), initial irradiation testing of candidate fuels can be performed in a thermal test reactor that has been modified to produce a test region with a hardened neutron spectrum. A test region is achieved with a Cadmium (Cd) filter which can harden the neutron spectrum to a spectrum similar (although still somewhat softer) to that of the liquid metal fast breeder reactor (LMFBR). A fuel test loop with a Cd-filter has been installed within the East Flux Trap (EFT) of the Advanced Test Reactor (ATR) at the Idaho National Laboratory (INL). A detailed comparison analyses between the cadmium (Cd) filter hardened neutron spectrum in the ATR and the LMFBR fast neutron spectrum have been performed using MCWO. MCWO is a set of scripting tools that are used to couple the Monte Carlo transport code MCNP with the isotope depletion and buildup code ORIGEN-2.2. The MCWO-calculated results indicate that the Cd-filter can effectively flatten the Rim-Effect and reduce the linear heat rate (LHGR) to meet the advanced fuel testing project requirements at the beginning of irradiation (BOI). However, the filtering characteristics of Cd as a strong absorber quickly depletes over time, and the Cd-filter must be replaced for every two typical operating cycles within the EFT of the ATR. The designed Cd-filter can effectively depress the LHGR in experimental fuels and harden the neutron spectrum enough to adequately flatten the Rim-Effect in the test region. (author)

  2. AGR-3/4 Final Data Qualification Report for ATR Cycles 151A through 155B-1

    Energy Technology Data Exchange (ETDEWEB)

    Pham, Binh T. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-03-01

    This report provides the qualification status of experimental data for the entire Advanced Gas Reactor 3/4 (AGR 3/4) fuel irradiation. AGR-3/4 is the third in a series of planned irradiation experiments conducted in the Advanced Test Reactor (ATR) at Idaho National Laboratory (INL) for the AGR Fuel Development and Qualification Program, which supports development of the advanced reactor technology under the INL ART Technology Development Office (TDO). The main objective of AGR-3/4 irradiation is to provide a known source of fission products for subsequent transport through compact matrix and structural graphite materials due to the presence of designed-to-fail fuel particles. Full power irradiation of the AGR 3/4 test began on December 14, 2011 (ATR Cycle 151A), and was completed on April 12, 2014 (end of ATR Cycle 155B) after 369.1 effective full power days of irradiation. The AGR-3/4 test was in the reactor core for eight of the ten ATR cycles between 151A and 155B. During the unplanned outage cycle, 153A, the experiment was removed from the ATR northeast flux trap (NEFT) location and stored in the ATR canal. This was to prevent overheating of fuel compacts due to higher than normal ATR power during the subsequent Powered Axial Locator Mechanism cycle, 153B. The AGR 3/4 test was inserted back into the ATR NEFT location during the outage of ATR Cycle 154A on April 26, 2013. Therefore, the AGR-3/4 irradiation data received during these 2 cycles (153A and 153B) are irrelevant and their qualification status isnot included in this report. Additionally, during ATR Cycle 152A the ATR core ran at low power for a short enough duration that the irradiation data are not used for physics and thermal calculations. However, the qualification status of irradiation data for this cycle is still covered in this report. As a result, this report includes data from 8 ATR Cycles: 151A, 151B, 152A, 152B, 154A, 154B, 155A, and 155B, as recorded in the Nuclear Data Management and

  3. Correlation Study of PVDF Membrane Morphology with Protein Adsorption: Quantitative Analysis by FTIR/ATR Technique

    Science.gov (United States)

    Ideris, N.; Ahmad, A. L.; Ooi, B. S.; Low, S. C.

    2018-05-01

    Microporous PVDF membranes were used as protein capture matrices in immunoassays. Because the most common labels in immunoassays were detected based on the colour change, an understanding of how protein concentration varies on different PVDF surfaces was needed. Herein, the correlation between the membrane pore size and protein adsorption was systematically investigated. Five different PVDF membrane morphologies were prepared and FTIR/ATR was employed to accurately quantify the surface protein concentration on membranes with small pore sizes. SigmaPlot® was used to find a suitable curve fit for protein adsorption and membrane pore size, with a high correlation coefficient, R2, of 0.9971.

  4. Radar automatic target recognition (ATR) and non-cooperative target recognition (NCTR)

    CERN Document Server

    Blacknell, David

    2013-01-01

    The ability to detect and locate targets by day or night, over wide areas, regardless of weather conditions has long made radar a key sensor in many military and civil applications. However, the ability to automatically and reliably distinguish different targets represents a difficult challenge. Radar Automatic Target Recognition (ATR) and Non-Cooperative Target Recognition (NCTR) captures material presented in the NATO SET-172 lecture series to provide an overview of the state-of-the-art and continuing challenges of radar target recognition. Topics covered include the problem as applied to th

  5. Identification of herbarium whole-leaf samples of Epilobium species by ATR-IR spectroscopy.

    Science.gov (United States)

    Strgulc Krajsek, Simona; Buh, Primoz; Zega, Anamarija; Kreft, Samo

    2008-02-01

    A simple, high-accuracy FT-IR method based on attenuated total reflection (ATR) was developed for the rapid determination of leaf samples of Epilobium species. The method is superior to other analytical techniques, since there is no need of laborious sample preparation such as grinding or extraction and solvent removal. A total of 70 herbarium specimens, belonging to all 13 Epilobium and to 2 Chamerion species growing in Slovenia, were analyzed. With the 100 most-informative wavenumbers in the range 700-1800 cm(-1), we obtained over 90% accuracy of species identification, with discriminant multivariate statistical analysis on the measurements made on whole dried leaves.

  6. Measured thermal and fast neutron fluence rates ATR Cycle 101-B, October 11, 1993--November 27, 1993

    International Nuclear Information System (INIS)

    Murray, R.K.; Rogers, J.W.

    1994-01-01

    This report contains the thermal (2200 m/s) and fast (E>lMeV) neutron fluence rate data for ATR Cycle 101-B which were measured by the Radiation Measurements Laboratory (RML) as requested by the Power Reactor Programs (ATR Experiments) Radiation Measurements Work Order. This report contains fluence rate values corresponding to the particular elevations (relative to the 80 ft. core elevation) where the measurements were taken. The data in this report consists of (1) a table of the ATR power history and distribution, (2) a hard copy listing of all thermal and fast neutron fluence rates, (3) plots of both the thermal and fast neutron fluence rates, and (4) a magnetic record (3.5 inch diskette) containing a listing of only the fast neutron fluence rates, their assigned elevations proper header identification of all monitor positions contained herein

  7. Investigation on the adsorption characteristics of sodium benzoate and taurine on gold nanoparticle film by ATR-FTIR spectroscopy

    Science.gov (United States)

    Kumar, Naveen; Thomas, S.; Tokas, R. B.; Kshirsagar, R. J.

    2014-01-01

    Fourier transform infrared (FTIR) spectroscopic studies of sodium benzoate and taurine adsorbed on gold nanoparticle (AuNp) film on silanised glass slides have been studied by attenuated total reflection technique (ATR). The surface morphology of the AuNp films has been measured by Atomic Force Microscopy. The ATR spectra of sodium benzoate and taurine deposited on AuNp film are compared with ATR spectra of their powdered bulk samples. A new red-shifted band appeared along with the symmetric and asymmetric stretches of carboxylate group of sodium benzoate leading to a broadening of the above peaks. Similar behavior is also seen in the case of symmetric and asymmetric stretches of sulphonate group of taurine. The results indicate presence of both chemisorbed and physisorbed layers of both sodium benzoate and taurine on the AuNp film with bottom layer chemically bound to AuNp through carboxylate and sulphonate groups respectively.

  8. Profil Penalaran Proporsional Siswa SMP dalam Memecahkan Masalah Matematika Berdasarkan Gaya Kognitif Sistematis dan Intuitif

    Directory of Open Access Journals (Sweden)

    Akhmad Faisal Hidayat

    2017-12-01

    Full Text Available Penelitian ini merupakan penelitian deskriptif dengan pendekatan kualitatif. Tujuan penelitian ini adalah mendeskripsikan profil penalaran proporsional siswa SMP dalam memecahkan masalah matematika berdasarkan gaya kognitif sistematis dan intuitif. Penelitian ini dilakukan di kelas IX SMP Al-Muslim Sidoarjo pada tahun ajaran 2016/2017. Subjek penelitian ini adalah seorang siswa bergaya kognitif sistematis dan seorang siswa bergaya kognitif sistematis. Alat pengumpul data berupa Tes Gaya Kognitif (TGK, Tugas Pemecahan Masalah (TPM, Pedoman wawancara, dan alat rekam audio visual. Data penelitian diperoleh dari pemberian tugas pemecahan masalah dan wawancara  sebanyak dua kali. Wawancara dilakukan untuk mengungkapkan profil penalaran proporsional dalam memecahkan masalah matematika terkait materi perbandingan. Keabsahan data diuji dengan triangulasi waktu. Hasil penelitian menunjukkan kedua subjek gagal membedakan masalah proporsional dan bukan proporsional pada tahap memahami masalah. Pada tahap menyusun rencana, Siswa bergaya kognitif sistematis mengelompokkan bagian-bagian yang sebanding untuk membuat persamaan. Sedangkan siswa bergaya kognitif intuitif membandingkan luas lahan pertama dan kedua, kemudian menyederhanakan perbandingannya. Pada tahap melaksanakan rencana, siswa bergaya kognitif sistematis menggunakan strategi cross product algorithm untuk menemukan solusi. Sedangkan siswa bergaya kognitif intuitif menggunakan strategi build-up method dan factor of change untuk menemukan solusi. Pada tahap memeriksa kembali, Siswa bergaya konitif sistematis dan intuitif mengecek solusi yang mereka peroleh dengan mensubtitusi masing-masing solusi ke persamaan, kemudian melihat nilai perbandingan yang dihasilkan. Jawaban benar jika nilai perbandingan dalam persaamaan tersebut sama.   This research uses descriptive qualitative approach. The purpose of this study was to describe proportional reasoning profile of junior high school student in

  9. Analysis code for large rupture accidents in ATR. SENHOR/FLOOD/HEATUP

    International Nuclear Information System (INIS)

    1997-08-01

    In the evaluation of thermo-hydraulic transient change, the behavior of core reflooding and the transient change of fuel temperature in the events which are classified in large rupture accidents of reactor coolant loss, that is the safety evaluation event of the ATR, the analysis codes for thermo-hydraulic transient change at the time of large rupture SENHOR, for core reflooding characteristics FLOOD and for fuel temperature HEATUP are used, respectively. The analysis code system for loss of coolant accident comprises the analysis code for thermo-hydraulic transient change at the time of medium and small ruptures LOTRAC in addition to the above three codes. Based on the changes with time lapse of reactor thermal output and steam drum pressure obtained by the SENHOR, average reflooding rate is analyzed by the FLOOD, and the time of starting the turnaround of fuel cladding tube temperature and the heat transfer rate after the turnaround are determined. Based on these data, the detailed temperature change of fuel elements is analyzed by the HEATUP, and the highest temperature and the amount of oxidation of fuel cladding tubes are determined. The SENHOR code, the FLOOD code and the HEATUP code and various models for these codes are explained. The example of evaluation and the sensitivity analysis of the ATR plant are reported in the Appendix. (K.I.)

  10. Optical fingerprints of solid-liquid interfaces: a joint ATR-IR and first principles investigation

    Science.gov (United States)

    Yang, L.; Niu, F.; Tecklenburg, S.; Pander, M.; Nayak, S.; Erbe, A.; Wippermann, S.; Gygi, F.; Galli, G.

    Despite the importance of understanding the structural and bonding properties of solid-liquid interfaces for a wide range of (photo-)electrochemical applications, there are presently no experimental techniques available to directly probe the microscopic structure of solid-liquid interfaces. To develop robust strategies to interpret experiments and validate theory, we carried out attenuated total internal reflection (ATR-IR) spectroscopy measurements and ab initio molecular dynamics (AIMD) simulations of the vibrational properties of interfaces between liquid water and well-controlled prototypical semiconductor substrates. We show the Ge(100)/H2O interface to feature a reversible potential-dependent surface phase transition between Ge-H and Ge-OH termination. The Si(100)/H2O interface is proposed as a model system for corrosion and oxidation processes. We performed AIMD calculations under finite electric fields, revealing different pathways for initial oxidation. These pathways are predicted to exhibit unique spectral signatures. A significant increase in surface specificity can be achieved utilizing an angle-dependent ATR-IR experiment, which allows to detect such signatures at the interfacial layer and consequently changes in the hydrogen bond network. Funding from DOE-BES Grant No. DE-SS0008939 and the Deutsche Forschungsgemeinschaft (RESOLV, EXC 1069) are gratefully acknowledged.

  11. In-column ATR-FTIR spectroscopy to monitor affinity chromatography purification of monoclonal antibodies

    Science.gov (United States)

    Boulet-Audet, Maxime; Kazarian, Sergei G.; Byrne, Bernadette

    2016-01-01

    In recent years many monoclonal antibodies (mAb) have entered the biotherapeutics market, offering new treatments for chronic and life-threatening diseases. Protein A resin captures monoclonal antibody (mAb) effectively, but the binding capacity decays over repeated purification cycles. On an industrial scale, replacing fouled Protein A affinity chromatography resin accounts for a large proportion of the raw material cost. Cleaning-in-place (CIP) procedures were developed to extend Protein A resin lifespan, but chromatograms cannot reliably quantify any remaining contaminants over repeated cycles. To study resin fouling in situ, we coupled affinity chromatography and Fourier transform infrared (FTIR) spectroscopy for the first time, by embedding an attenuated total reflection (ATR) sensor inside a micro-scale column while measuring the UV 280 nm and conductivity. Our approach quantified the in-column protein concentration in the resin bed and determined protein conformation. Our results show that Protein A ligand leached during CIP. We also found that host cell proteins bound to the Protein A resin even more strongly than mAbs and that typical CIP conditions do not remove all fouling contaminants. The insights derived from in-column ATR-FTIR spectroscopic monitoring could contribute to mAb purification quality assurance as well as guide the development of more effective CIP conditions to optimise resin lifespan. PMID:27470880

  12. New particle formation events measured on board the ATR-42 aircraft during the EUCAARI campaign

    Directory of Open Access Journals (Sweden)

    S. Crumeyrolle

    2010-07-01

    Full Text Available Aerosol properties were studied during an intensive airborne measurement campaign that took place at Rotterdam in Netherlands in May 2008 within the framework of the European Aerosol Cloud Climate and Air Quality Interactions project (EUCAARI. The objective of this study is to illustrate seven events of new particle formation (NPF observed with two Condensation Particle Counters (CPCs operated on board the ATR-42 research aircraft in airsectors around Rotterdam, and to provide information on the spatial extent of the new particle formation phenomenon based on 1-s resolution measurements of ultra-fine particle (in the size range 3–10 nm diameter, denoted N3-10 hereafter concentrations. The results show that particle production occurred under the influence of different air mass origins, at different day times and over the North Sea as well as over the continent. The number concentration of freshly nucleated particles (N3-10 varied between 5000 and 100 000 cm−3 within the boundary layer (BL. Furthermore the vertical extension for all nucleation events observed on the ATR-42 never exceeded the upper limit of the BL. The horizontal extent of N3-10 could not be delimited due to inflexible flight plans which could not be modified to accommodate real-time results. However, the NPF events were observed over geographically large areas; typically the horizontal extension was about 100 km and larger.

  13. Characterization of Printing Inks Using DART-Q-TOF-MS and Attenuated Total Reflectance (ATR) FTIR.

    Science.gov (United States)

    Williamson, Rhett; Raeva, Anna; Almirall, Jose R

    2016-05-01

    The rise in improved and widely accessible printing technology has resulted in an interest to develop rapid and minimally destructive chemical analytical techniques that can characterize printing inks for forensic document analysis. Chemical characterization of printing inks allows for both discrimination of inks originating from different sources and the association of inks originating from the same source. Direct analysis in real-time mass spectrometry (DART-MS) and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) were used in tandem to analyze four different classes of printing inks: inkjets, toners, offset, and intaglio. A total of 319 samples or ~ 80 samples from each class were analyzed directly on a paper substrate using the two methods. DART-MS was found to characterize the semi-volatile polymeric vehicle components, while ATR-FTIR provided chemical information associated with the bulk components of these inks. Complimentary data results in improved discrimination when both techniques are used in succession resulting in >96% discrimination for all toners, 95% for all inkjets, >92% for all offset, and >54% for all intaglio inks. © 2016 American Academy of Forensic Sciences.

  14. Centromeric DNA replication reconstitution reveals DNA loops and ATR checkpoint suppression.

    Science.gov (United States)

    Aze, Antoine; Sannino, Vincenzo; Soffientini, Paolo; Bachi, Angela; Costanzo, Vincenzo

    2016-06-01

    Half of the human genome is made up of repetitive DNA. However, mechanisms underlying replication of chromosome regions containing repetitive DNA are poorly understood. We reconstituted replication of defined human chromosome segments using bacterial artificial chromosomes in Xenopus laevis egg extract. Using this approach we characterized the chromatin assembly and replication dynamics of centromeric alpha-satellite DNA. Proteomic analysis of centromeric chromatin revealed replication-dependent enrichment of a network of DNA repair factors including the MSH2-6 complex, which was required for efficient centromeric DNA replication. However, contrary to expectations, the ATR-dependent checkpoint monitoring DNA replication fork arrest could not be activated on highly repetitive DNA due to the inability of the single-stranded DNA binding protein RPA to accumulate on chromatin. Electron microscopy of centromeric DNA and supercoil mapping revealed the presence of topoisomerase I-dependent DNA loops embedded in a protein matrix enriched for SMC2-4 proteins. This arrangement suppressed ATR signalling by preventing RPA hyper-loading, facilitating replication of centromeric DNA. These findings have important implications for our understanding of repetitive DNA metabolism and centromere organization under normal and stressful conditions.

  15. Analytical characterization of polymers used in conservation and restoration by ATR-FTIR spectroscopy.

    Science.gov (United States)

    Chércoles Asensio, Ruth; San Andrés Moya, Margarita; de la Roja, José Manuel; Gómez, Marisa

    2009-12-01

    In the last few decades many new polymers have been synthesized that are now being used in cultural heritage conservation. The physical and chemical properties and the long-term behaviors of these new polymers are determined by the chemical composition of the starting materials used in their synthesis along with the nature of the substances added to facilitate their production. The practical applications of these polymers depend on their composition and form (foam, film, sheets, pressure-sensitive adhesives, heat-seal adhesives, etc.). Some materials are used in restoration works and others for the exhibition, storage and transport of works of art. In all cases, it is absolutely necessary to know their compositions. Furthermore, many different materials that are manufactured for other objectives are also used for conservation and restoration. The technical information about the materials provided by the manufacturer is usually incomplete, so it is necessary to analytically characterize such materials. FTIR spectrometry is widely used for polymer identification, and, more recently, ATR-FTIR has been shown to give excellent results. This paper reports the ATR-FTIR analysis of samples of polymeric materials used in the conservation of artworks. These samples were examined directly in the solid material without sample preparation.

  16. In-column ATR-FTIR spectroscopy to monitor affinity chromatography purification of monoclonal antibodies

    Science.gov (United States)

    Boulet-Audet, Maxime; Kazarian, Sergei G.; Byrne, Bernadette

    2016-07-01

    In recent years many monoclonal antibodies (mAb) have entered the biotherapeutics market, offering new treatments for chronic and life-threatening diseases. Protein A resin captures monoclonal antibody (mAb) effectively, but the binding capacity decays over repeated purification cycles. On an industrial scale, replacing fouled Protein A affinity chromatography resin accounts for a large proportion of the raw material cost. Cleaning-in-place (CIP) procedures were developed to extend Protein A resin lifespan, but chromatograms cannot reliably quantify any remaining contaminants over repeated cycles. To study resin fouling in situ, we coupled affinity chromatography and Fourier transform infrared (FTIR) spectroscopy for the first time, by embedding an attenuated total reflection (ATR) sensor inside a micro-scale column while measuring the UV 280 nm and conductivity. Our approach quantified the in-column protein concentration in the resin bed and determined protein conformation. Our results show that Protein A ligand leached during CIP. We also found that host cell proteins bound to the Protein A resin even more strongly than mAbs and that typical CIP conditions do not remove all fouling contaminants. The insights derived from in-column ATR-FTIR spectroscopic monitoring could contribute to mAb purification quality assurance as well as guide the development of more effective CIP conditions to optimise resin lifespan.

  17. ATR-IR study of skin components: Lipids, proteins and water. Part I: Temperature effect

    Science.gov (United States)

    Olsztyńska-Janus, S.; Pietruszka, A.; Kiełbowicz, Z.; Czarnecki, M. A.

    2018-01-01

    In this work we report the studies of the effect of temperature on skin components, such as lipids, proteins and water. Modifications of lipids structure induced by increasing temperature (from 20 to 90 °C) have been studied using ATR-IR (Attenuated Total Reflectance Infrared) spectroscopy, which is a powerful tool for characterization of the molecular structure and properties of tissues, such as skin. Due to the small depth of penetration (0.6-5.6 μm), ATR-IR spectroscopy probes only the outermost layer of the skin, i.e. the stratum corneum (SC). The assignment of main spectral features of skin components allows for the determination of phase transitions from the temperature dependencies of band intensities [e.g. νas(CH2) and νs(CH2)]. The phase transitions were determined by using two methods: the first one was based on the first derivative of the Boltzmann function and the second one employed tangent lines of sigmoidal, aforementioned dependencies. The phase transitions in lipids were correlated with modifications of the structure of water and proteins.

  18. Prediction of the oversulphated chondroitin sulphate contamination of unfractionated heparin by ATR-IR spectrophotometry.

    Science.gov (United States)

    Norwig, J; Beyer, T; Brinz, D; Holzgrabe, U; Diller, M; Manns, D

    2009-03-01

    The detection of a contamination of heparin with oversulphated chondroitin sulphate (OSCS) was first analysed in an unfractionated heparin batch supplied to the US API-market in April 2006. OSCS is a semi-synthetic derivative of the natural occuring glycosaminoglycan chondroitin sulphate. Moreover some spectroscopic characteristics of the substance overlap with those of heparin, so that the infrared (IR) spectra are visually difficult to distinguish whereas (1)H-NMR (Nuclear Magnetic Resonance) spectroscopy or capillary electrophoresis (CE) provides identification by a simple visual inspection of either the spectrum or the electropherogram respectively. However, applying special tools of Multivariate Data Analysis (MVA) to the IR spectra an identification of the contaminated samples is possible. In detail a rapid Attenuation Total Reflectance-Infrared (ATR-IR) measurement was selected, which does not require any sample preparation. The result (contaminated or not contaminated) is predicted within a few minutes. A method transfer to mobile ATR-IR spectrometers seems to be possible. The analysis is based on the fact that the fingerprint of the OSCS IR spectrum (1st derivative) complies with a theoretically calculated principal component in the MVA.

  19. ANALISIS TERHADAP PERLUNYA PENYESUAIAN LAPORAN KEUANGAN HISTORIS (CONVENTIONAL ACCOUNTING MENJADI BERDASARKAN TINGKAT HARGA UMUM (GENERAL PRICE LEVEL ACCOUNTING

    Directory of Open Access Journals (Sweden)

    Pwee Leng

    2002-01-01

    Full Text Available Generally, in conventional accounting, financial statements are based on the historical cost principle that assumes that prices (monetery unit are stable. Conventional accounting recognizes neither changes in the general price level nor changes in the specific price level. Consequently, if there are any changes in purchasing power such as in inflation period, the historical financial statement are not economically relevant and also income is usually overstated, and the fixed assets are usually understated. Actually, there are several methods on accounting for the effect of changing prices, such as constant accounting, current value accounting, and general price level accounting. General price level accounting will do restatement the components of financial statement to be a rupiah on a similar level of purchasing power, but without changes in accounting principles which using on conventional accounting. In practice, the controversy concerning the relevance of general price level accounting has been continuing. Pros and cons general price level accounting will be presented on this paper. Also the result of two researches concerning the influence of applied general price level accounting on the financial statement will be compared as considerations whether the general price level adjustment is necessary needed. Abstract in Bahasa Indonesia : Secara umum, dalam akuntansi konvensional, laporan keuangan disajikan berdasarkan nilai historis yang mengasumsikan bahwa harga-harga (unit moneter adalah stabil. Akuntansi konvensional tidak mengakui adanya perubahan tingkat harga umum maupun perubahan tingkat harga khusus. Sebagai konsekuensinya, jika terjadi perubahan daya beli seperti pada periode inflasi, maka laporan keuangan historis secara ekonomis tidaklah relevan. Pada periode ini pendapatan umumnya dinilai lebih tinggi sedangkan aktiva tetap dinilai lebih rendah. Sebenarnya, terdapat beberapa metode akuntansi mengenai pengaruh perubahan harga

  20. Analisis Deskriptif Soal Geometri dalam Buku Matematika Bilingual untuk Sekolah Menengah Pertama Kelas VIII Berdasarkan Kriteria International Assessment TIMSS 2007

    Directory of Open Access Journals (Sweden)

    Etik Rahayu

    2012-06-01

    Full Text Available Tujuan penelitian ini adalah untuk mendeskripsikan  domain kognitif dan aspek kognitif (required behavior soal matematika dalam Buku Matematika Bilingual untuk Sekolah Menengah Pertama (SMP Kelas VIII berdasarkan kriteria International Assessment TIMSS 2007 dan proporsinya. Metode penelitian yang digunakan adalah deskriptif kualitatif, dengan subjek penelitian adalah soal geometri dalam Buku Matematika Bilingual SMP. yang berjudul “Mathematics for Junior High School Grade VIII 1st Semester” dan “Mathematics for Junior High School Grade VIII 2nd Semester” karangan karangan M. Cholik Adinawan dan Sugijono. Pengumpulan data menggunakan metode observasi dan wawancara tentang penggunaan buku matematika bilingual yang paling banyak digunakan di kota semarang. Pedoman analisis soal berdasarkan kriteria International Assessment TIMSS 2007, dengan validasi hasil oleh ahli. Hasil penelitian menunjukkan bahwa soal yang dianalisis memuat satu hingga tujuh aspek kognitif. Sebagian besar soal memuat 4 aspek kognitif yaitu 44.04 %, diikuti soal dengan 3 aspek kognitif  yaitu 36, 42%,  soal dengan 2 aspek kognitif yaitu 14, 90%, kemudian 1,99% untuk soal dengan 1 atau 5 aspek kognitif, dan 0,33% untuk soal dengan 6 atau 7 aspek kognitif. Proporsi tinggi pada recall (28.26% dan compute (26.57%, diikuti dengan SRP (10.85%, implement (10.65%, retrieve (8.36%, recognize (6.17%, analyze (1.99%, measure (1.59%, generalize (1.09%, SNRP (1.00%, classify (0.80%, represent (0.80%, justify (0.80%, select (0.60%, model (0.30%, synthesis (0.20%. Secara keseluruhan berdasarkan International Assessment TIMSS 2007 soal yang termasuk domain knowing memiliki persentase paling tinggi (52.28%, domain knowing-applying (24.83%, domain knowing- reasoning (12.91%, dan hanya sedikit yang termasuk domain knowing-apllying-reasoning (3.97%. Serta terdapat 4 soal (1.32% yang mempunyai ketidaksesuaian penggunaan mathematics terms serta 1 soal (0

  1. IMPLIKASI YURIDIS PENETAPAN DIREKSI BPR BERBENTUK PT BERDASARKAN POJK NOMOR 20/POJK.03/2014 TENTANG BPR

    Directory of Open Access Journals (Sweden)

    Evina Dhana Hermansyah

    2016-04-01

    .  Dalam hal salah satu anggota Direksi BPR tidak terisi, maka BPR menetapkan pengganti anggota Direksi penggantinya kepada OJK berdasarkan POJK.POJK menetapkan jangka waktu 120 (seratus dua puluh hari kepada BPR untuk menetapkan Direksi pengganti. Nama calon anggota Direksi yang diajukan BPR kepada OJK tidak serta merta diterima oleh OJK. Calon anggota Direksi yang diajukan tersebut harus memenuhi kriteria dan persyaratan yang ditetapkan oleh OJK. Jurnal ini ditulis untukmenganalisis implikasi yang timbul terhadap BPR sehubungan dengan penetapan anggota Direksi berdasarkan POJK. Selain itu, penulisan jurnal ini juga untuk mengetahui bagaimana bentuk perbuatan hukum yang dilakukan selama susunan anggota Direksinya belum memenuhi ketentuan POJK.Dalam beberapa kasus, BPR dapat berkali-kali mengajukan permohonan calon anggota Direksinya sehingga BPR melewati batas waktu yang ditentukan dalam POJK untuk memenuhi jumlah minimum anggota Direksi. Secara normatif berdasarkan POJK, BPR dapat dikenai sanksi atas tidak terpenuhinya jumlah minimum anggota Direksi walaupun hal tersebut bukanlah mutlak kesalahan BPR.Jurnal ini disusun dengan metode penelitian yuridis normatif dengan pendekatan peraturan perundang-undangan. Berdasarkan hasil penelitian, ketentuan dalam POJK ini sehubungan dengan penetapan anggota Direksi BPR masih belum memberikan kepastian hukum bagi BPR. Ketentuan di dalam POJK terkait dengan pelaksanaan peneteapan anggota Direksi BPR  dapat menyebabkan BPR tidak memenuhi jumlah minimum anggota Direksi untuk jangka waktu yang relatif lama. BPR yang tidak memenuhi jumlah minimal anggota Direksi menyebabkan BPR tersebut tidak memiliki organ yang utuh sebagai suatu badan hukum. Kata kunci: peraturan OJK, BPR, penetapan direksi

  2. Analisis Isi SMS Iklan Layanan Telekomunikasi Operator Telkomsel, Indosat dan Xl berdasarkan Etika Pariwara Indonesia Periode 2013

    Directory of Open Access Journals (Sweden)

    Danang Trijayanto

    2014-09-01

    Full Text Available Perkembangan teknologi komunikasi merevolusi aktivitas pemasaran ke mobile marketing, salah satunya adalah dengan SMS advertising. Badan usaha yang cenderung menggunakan cara pemasaran tersebut adalah operator selular dengan iklan produk layanan telekomunikasinya. Permasalahannya adalah strategi pemasaran produk telekomunikasi yang cenderung banyak merugikan pelanggan karena ketidaksesuaian antara isi iklan dan produk yang diperoleh, yang dipengaruhi oleh kelengkapan dan kejelasan informasi produk. Salah satu aturan yang menjadi dasar dalam aktivitas SMS advertising adalah berdasarkan Etika Pariwara Indonesia.  Penelitian  ini   meneliti  bagaimanakah  Penerapan aturan tersebut terhadap isi SMS iklan produk telekomunikasi yang dikirimkan oleh provider selular GSM prabayar pada bulan Juli hingga Nopember 2013.  Hasil  Penelitian menunjukkan bahwa dalam mengiklankan produk telekomunikasi melalui SMS advertising, para operator selular belum menerapkan regulasi dan etika pemasaran dengan baik. Kekurangan informasi yang belum lengkap terdapat pada informasi syarat kontrak yang menjadi informasi dasar hak dan kewajiban yang harus dipenuhi oleh pelanggan.

  3. KEEFEKTIFAN PEMBELAJARAN KETERAMPILAN MENULIS CERPEN DENGAN MODEL INVESTIGASI KELOMPOK DAN MODEL STAD BERDASARKAN TIPE KEPRIBADIAN PESERTA DIDIK KELAS VII

    Directory of Open Access Journals (Sweden)

    Agus Pujianto

    2015-03-01

    Full Text Available Tujuan penelitian ini adalah menguji keefektifan pembelajaran keterampilan menulis cerpen dengan model investigasi kelompok dan model STAD berdasarkan tipe kepribadian peserta didik kelas VII. Keterampilan menulis cerpen membutuhkan kreativitas dalam pelaksanaan pembelajaran. Melalui keterampilan menulis cerpen siswa dapat berkreasi untuk mengemukakan ide atau gagasan yang ingin disampaikan kepada pembaca. Ide atau gagasan juga berpengaruh terhadap hasil dalam menulis cerpen. Selain itu, pengetahuan tentang cerpen juga penting dalam pembelajaran keterampilan menulis cerpen. Siswa perlu berpikir kritis untuk memunculkan ide atau gagasan yang menarik dan untuk pemahaman mengenai unsur-unsur cerpen. Tipe kepribadian yang berbeda juga perlu membutuhkan cara pengajaran yang berbeda. Oleh karena itu, dibutuhkan model pembelajaran yang cocok. Model pembelajaran yang dipilih adalah model investigasi kelompok dan model STAD.

  4. Klasifikasi Topik Keluhan Pelanggan Berdasarkan Tweet dengan Menggunakan Penggabungan Feature Hasil Ekstraksi pada Metode Support Vector Machine (SVM

    Directory of Open Access Journals (Sweden)

    Enda Esyudha Pratama

    2015-12-01

    Full Text Available Pemanfaatan twitter sebagai layanan customer serevice perusahaan sudah mulai banyak digunakan, tak terkecuali Speedy. Mekanisme yang ada saat ini untuk proses klasifikasi bentuk dan jenis keluhan serta informasi tentang jumlah keluhan lewat twitter masih dilakukan secara manual. Belum lagi data twitter yang bersifat tidak terstruktur tentunya akan menyulitkan untuk dilakukan analisa dan penggalian informasi dari data tersebut. Berdasarkan permasalahan tersebut, penelitian ini bertujuan untuk memproses data teks dari tweet pengguna twitteryang masuk ke akun @TelkomSpeedy untuk diolah menjadi informasi. Informasi tersebut nantinya digunakan untuk klasifikasi bentuk dan jenis keluhan. Merujuk pada beberapa penelitian terkait, salah satu metode klasifikasi yang paling baik untuk digunakan adalah metode Support Vector Machine (SVM. Konsep dari SVM dapat dijelaskan secara sederhana sebagai usaha mencari hyperplane yang dapat memisahkan dataset sesuai dengan kelasnya. Kelas yang digunakan dalam penelitian kali ini berdasarkan topik keluhan pelanggan yaitu billing, pemasangan/instalasi, putus (disconnect, dan lambat. Faktor penting lainnya dalam hal klasifikasi adalah penentuan feature atau atribut kata yang akan digunakan. Metode feature selection yang digunakan pada penlitian ini adalah term frequency (TF, document frequency (DF, information gain, dan chi-square. Pada penelitian ini juga dilakukan metode penggabungan feature yang telah dihasilkan dari beberapa metode feature selection sebelumnya. Dari hasil penelitian menunjukan bahwa SVM mampu melakukan klasifikasi keluhan dengan baik, hal ini dibuktikan dengan akurasi 82,50% untuk klasifikasi bentuk keluhan dan 86,67% untuk klasifikasi jenis keluhan. Sedangkan untuk kombinasi penggunaan feature dapat meningkatkan akurasi menjadi 83,33% untuk bentuk keluhan dan 89,17% untuk jenis keluhan.   Kata Kunci—customer service, klasifikasi topik keluhan, penggabungan feature, support vector machine

  5. Rancang Bangun Alat Uji Komposisi Massa Pada Logam Paduan Berdasarkan Prinsip Gaya Angkat Fluida

    Directory of Open Access Journals (Sweden)

    Agus Slamet

    2012-02-01

    Full Text Available Spectro Analyser merupakan alat uji komposisi yang sangat presisi dan harganya sangat mahal, maka diperlukan alat uji komposisi yang lebih sederhana dan murah tapi dapat berfungsi sebagai alat uji komposisi logam paduan yang terdiri dua unsur saja.                 Prinsip kerja dari rancang bangun Alat Uji Komposisi Massa ini adalah berdasarkan benda terbenam atau terapung pada permukaan fluida cair maka gaya yang bekerja pada benda tersebut terjadi karena tekanan fluida cair atau disebut sebagai gaya angkat (bouyancy.,                 Pengujian ini akan mendapatkan data dari alat uji adalah volume air yang dipindahkan atau volume bahan uji dan massa bahan uji saat di dalam fluida sebagai representasi dari resultan gaya..                 Metode penelitian yang dilakukan dimulai dari perancangan dan pembuatan alat ,kemudian dilanjutkan dengan pengujian, pengambilan data dengan cara mencelupkan bahan uji ke dalam fluida pada gelas ukur. Bahan uji yang digunakan mengandung dua unsur yaitu alumunium (Al dan zink (Zn dengan komposisi massa Bahan uji A :76,35% Al ;23,65% Zn, bahan uji B : 73,42% Al ;26,58% Zn dan bahan uji C: 83,38% Al ; 16,62% Zn. Dari hasil pengukuran dihitung ketidakpastian hasil pengukuran dari alat uji.                 Hasil pengujian dan data yang diperoleh dapat diketahui bahwa Alat uji komposisi massa dapat berfungsi untuk mengukur logam dengan paduan dua unsur dengan luaran massa bahan uji sebelum maupun saat di dalam fluida (air dan volume air yang dipindahkan atau volume bahan uji. Dari ketiga bahan uji menghasilkan perbedaan nilai komposisi massa dibandingkan dengan nilai yang sudah ditentukan. Nilai perbedaan untuk bahan uji A,B dan C berturut-turut 3.31%, 5,10% dan 4,96%, sedangkan alat uji ini mempunyai ketidakpastian hasil pengukurannya sebesar 3,33%. Kata kunci : komposisi, massa,volume     The Spectro Analyzer is a very accurate and expensive test device

  6. ATR-FTIR Spectroscopy Highlights the Problem of Distinguishing Between Exophiala dermatitidis and E. phaeomuriformis Using MALDI-TOF MS

    NARCIS (Netherlands)

    Ergin, C.; Gok, Y.; Baygu, Y.; Gumral, R.; Ozhak-Baysan, B.; Dogen, A.; Ogunc, D.; Ilkit, M.; Seyedmousavi, S.

    2016-01-01

    The present study compared two chemical-based methods, namely, matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, to understand the misidentification of Exophiala

  7. Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) in the discrimination of normal and oral cancer blood plasma

    Science.gov (United States)

    Pachaiappan, Rekha; Prakasarao, Aruna; Singaravelu, Ganesan

    2017-02-01

    Oral cancer is the most frequent type of cancer that occurs with 75000 to 80000 new cases reported every year in India. The carcinogens from tobacco and related products are the main cause for the oral cancer. ATR-FTIR method is label free, fast and cost-effective diagnostic method would allow for rapid diagnostic results in earlier stages by the minimal chemical changes occur in the biological metabolites available in the blood plasma. The present study reports the use of ATR-FTIR data with advanced statistical model (LDA-ANN) in the diagnosis of oral cancer from normal with better accuracy. The infrared spectra were acquired on ATR-FTIR Jasco spectrophotometer at 4 cm-1 resolution, 30 scans, in the 1800-900 cm-1 spectral range. Each sample had 5 spectra recorded from each blood plasma sample. The spectral data were routed through the multilayer perception of artificial neural network to evaluate for the statistical efficacy. Among the spectral data it was found that amide II (1486 cm-1) and lipid (1526 cm-1) affords about 90 % in the discrimination between groups using LDA. These preliminary results indicate that ATR-FTIR is useful to differentiate normal subject from oral cancer patients using blood plasma.

  8. ATR-IR spectroscopic cell for in situ studies at solid-liquid interface at elevated temperatures and pressures

    NARCIS (Netherlands)

    Koichumanova, Kamila; Visan, Aura; Geerdink, Bert; Lammertink, Rob G.H.; Mojet, Barbara; Seshan, Kulathuiyer; Lefferts, Leonardus

    2017-01-01

    An in situ ATR-IR spectroscopic cell suitable for studies at solid-liquid interface is described including the design and experimental details in continuous flow mode at elevated temperatures (230 °C) and pressures (30 bar). The design parameters considered include the cell geometry, the procedure

  9. Small Molecules Targeting Ataxia Telangiectasia and Rad3-Related (ATR) Kinase: An Emerging way to Enhance Existing Cancer Therapy

    Czech Academy of Sciences Publication Activity Database

    Andrs, M.; Korábečný, J.; Nepovimova, E.; Jun, D.; Hodný, Zdeněk; Kuca, K.

    2016-01-01

    Roč. 16, č. 3 (2016), s. 200-208 ISSN 1568-0096 Institutional support: RVO:68378050 Keywords : Ataxia telangiectasia and Rad3-related kinase (ATR) * cancer * chemosensitization * DNA damage response * phosphatidylinositol 3-kinase-related protein kinases (PIKK) * radiosensitization * synthetic lethality Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.992, year: 2016

  10. Measured thermal and fast neutron fluence rates for ATF-1 holders during ATR cycle 158B/159A

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Larry Don [Idaho National Lab. (INL), Idaho Falls, ID (United States); Miller, David Torbet [Idaho National Lab. (INL), Idaho Falls, ID (United States); Walker, Billy Justin [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2016-11-01

    This report contains the thermal (2200 m/s) and fast (E>1MeV) neutron fluence rate data for the ATF-1 holders located in core for ATR Cycle 158B/159A which were measured by the Radiation Measurements Laboratory (RML).

  11. Characterization of southern yellow pine bark layers by Attenuated Total Reflectance (ATR) and Fourier Transform Infrared (FT-IR) Spectroscopy

    Science.gov (United States)

    Thomas L. Eberhardt

    2009-01-01

    The outer bark (rhytidome) of the southern yellow pines is a complex structure comprised of alternating layers of obliterated phloem and periderm tissues, with the latter comprised of three layers, those being phellem, phellogen, and phelloderm. An attenuated total reflectance (ATR) sampling accessory, coupled with a Fourier transform infrared (FTIR) spectrometer,...

  12. Status Summary of FY16 Atom Probe Tomography Studies on UCSB ATR-2 Irradiated RPV Steels

    Energy Technology Data Exchange (ETDEWEB)

    Wells, Peter [Idaho National Lab. (INL), Idaho Falls, ID (United States); Odette, G. Robert [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2016-05-01

    The University of California Santa Barbara-2 RPV Steel Irradiation experiment was awarded in 2010 by the Nuclear Science User Facility (formerly ATR NSUF) through a competitive peer review proposal process. The experiment involved irradiation of nearly 1300 samples distributed over 13 capsules. The major objective of this experiment was to better understand embrittlement behavior of reactor pressure steels at doses beyond which available data exists yet may be achieved if reactor operating licenses are extended beyond 60 years. The experiment was instrumented during irradiation and active temperature control was used to maintain the temperature at the design temperature. Six samples were selected from a large matrix of materials to perform atom probe tomography (APT) to look at formation of high dose phases. The nature and formation behavior of these phases is discussed.

  13. Sensing molecular properties by ATR-SPP Raman spectroscopy on electrochemically structured sensor chips

    International Nuclear Information System (INIS)

    Zerulla, D.; Isfort, G.; Koelbach, M.; Otto, A.; Schierbaum, K.

    2003-01-01

    The use of electrochemically structured Al surfaces as sensor arrays for combinatorial chemistry and its detection via microscopic laser techniques from very small volumes has been explored. The methodology is based on three different techniques which will be discussed separately: firstly, attenuated total reflection (ATR) is used in connection with surface-plasmon-polariton (SPP) excitation. A thin Al layer, evaporated on sapphire or quartz and covered with a naturally grown oxide layer, provides an optimum enhancement and confinement of the electrical field close to the surface. This is revealed by calculations and experimental data. Secondly, a Raman microscope is applied, enabling chemical spot analysis in the visible and UV range with a lateral resolution close to the diffraction limit. Finally, its application to investigate electrochemically structured Al films is discussed

  14. Usage of FTIR-ATR as Non-Destructive Analysis of Selected Toxic Dyes

    Directory of Open Access Journals (Sweden)

    Bartošová Alica

    2017-06-01

    Full Text Available The degradation of the environment which is due to the discharge of polluting wastewater from industrial sources poses a real problem in several countries. Textile industries use large volumes of water in their operations, discharging thus large volume of wastewater into the environment, most of which is untreated. The wastewater contains a variety of chemicals from various stages of process operations, including desizing, scouring, bleaching and dyeing. The main purpose of this paper is to introduce Infrared Spectrometry with Fourier transformation as a non-destructive method for study, identifation and rapid determination of selected representatives of cationic (Methylene Blue, azo (Congo Red, Eriochrome Black T and nitroso (Naphthol Green B dyes. In conjunction with the ATR technique, FTIR offers a reliable detection method of dyes without extraction by other dangerous substances.

  15. Detection of creatinine enriched on a surface imprinted polystyrene film using FT-ATR-IR.

    Science.gov (United States)

    Sreenivasan, K

    2006-01-01

    The surface of polystyrene (PS) was chemically modified by coating a thin layer of polyaniline (PANI) by oxidizing aniline using ammonium persulfate. Affinity sites for creatinine, a clinically relevant molecule, were created in the coated layer by adding creatinine as print molecules during the oxidation. The imprinted layer adsorbed creatinine was compared to non-imprinted surface reflecting the creation of creatinine-specific sites on the surface. The equilibrium was attained rapidly, indicating that a material of this kind is suitable for sensing applications. The adsorbed creatinine on the surface was detected using the technique of Fourier transform attenuated total internal reflection infra red spectroscopy (FT-ATR-IR). The results show that molecularly imprinted surface can enrich molecules of interest and the enriched molecules can be detected using FT-IR.

  16. Characterisation of 17th-18th centuries damask and gilt leathers by ATR-FTIR

    Directory of Open Access Journals (Sweden)

    Lina Falcão

    2018-01-01

    Full Text Available In this paper it is presented the characterisation of tannins used in the vegetable tanning of two types of European historic and decorative leathers from Portuguese collections, damask leathers and gilt leathers, dated from the 17th and 18th centuries. Extracts prepared from collected leather fibres were analysed by attenuated total reflectance – Fourier transform infrared (ATR-FTIR spectroscopy. Results were compared with data collected from different reference vegetable tanning materials which, according to historic and technical literature, were used in Europe during the Modern Age. This study allowed, in most cases, the characterisation of the tannins used to produce the studied decorative vegetable tanned leathers and, at the same time, the detection of chemical deterioration of leathers, particularly collagen hydrolysis, which was not visually perceived.

  17. Usage of FTIR-ATR as Non-Destructive Analysis of Selected Toxic Dyes

    Science.gov (United States)

    Bartošová, Alica; Blinová, Lenka; Sirotiak, Maroš; Michalíková, Anna

    2017-06-01

    The degradation of the environment which is due to the discharge of polluting wastewater from industrial sources poses a real problem in several countries. Textile industries use large volumes of water in their operations, discharging thus large volume of wastewater into the environment, most of which is untreated. The wastewater contains a variety of chemicals from various stages of process operations, including desizing, scouring, bleaching and dyeing. The main purpose of this paper is to introduce Infrared Spectrometry with Fourier transformation as a non-destructive method for study, identifation and rapid determination of selected representatives of cationic (Methylene Blue), azo (Congo Red, Eriochrome Black T) and nitroso (Naphthol Green B) dyes. In conjunction with the ATR technique, FTIR offers a reliable detection method of dyes without extraction by other dangerous substances. Spectral interpretation of dye spectra revealed valuable information about the identification and characterization of each group of dyes.

  18. Convolutional neural network using generated data for SAR ATR with limited samples

    Science.gov (United States)

    Cong, Longjian; Gao, Lei; Zhang, Hui; Sun, Peng

    2018-03-01

    Being able to adapt all weather at all times, it has been a hot research topic that using Synthetic Aperture Radar(SAR) for remote sensing. Despite all the well-known advantages of SAR, it is hard to extract features because of its unique imaging methodology, and this challenge attracts the research interest of traditional Automatic Target Recognition(ATR) methods. With the development of deep learning technologies, convolutional neural networks(CNNs) give us another way out to detect and recognize targets, when a huge number of samples are available, but this premise is often not hold, when it comes to monitoring a specific type of ships. In this paper, we propose a method to enhance the performance of Faster R-CNN with limited samples to detect and recognize ships in SAR images.

  19. ATR-101, a selective ACAT1 inhibitor, decreases ACTH-stimulated cortisol concentrations in dogs with naturally occurring Cushing's syndrome.

    Science.gov (United States)

    Langlois, Daniel K; Fritz, Michele C; Schall, William D; Bari Olivier, N; Smedley, Rebecca C; Pearson, Paul G; Bailie, Marc B; Hunt, Stephen W

    2018-05-02

    Cushing's syndrome in humans shares many similarities with its counterpart in dogs in terms of etiology (pituitary versus adrenal causes), clinical signs, and pathophysiologic sequelae. In both species, treatment of pituitary- and adrenal-dependent disease is met with limitations. ATR-101, a selective inhibitor of ACAT1 (acyl coenzyme A:cholesterol acyltransferase 1), is a novel small molecule therapeutic currently in clinical development for the treatment of adrenocortical carcinoma, congenital adrenal hyperplasia, and Cushing's syndrome in humans. Previous studies in healthy dogs have shown that ATR-101 treatment led to rapid, dose-dependent decreases in adrenocorticotropic hormone (ACTH) stimulated cortisol levels. The purpose of this clinical study was to investigate the effects of ATR-101 in dogs with Cushing's syndrome. ATR-101 pharmacokinetics and activity were assessed in 10 dogs with naturally-occurring Cushing's syndrome, including 7 dogs with pituitary-dependent disease and 3 dogs with adrenal-dependent disease. ATR-101 was administered at 3 mg/kg PO once daily for one week, followed by 30 mg/kg PO once daily for one (n = 4) or three (n = 6) weeks. Clinical, biochemical, adrenal hormonal, and pharmacokinetic data were obtained weekly for study duration. ATR-101 exposure increased with increasing dose. ACTH-stimulated cortisol concentrations, the primary endpoint for the study, were significantly decreased with responders (9 of 10 dogs) experiencing a mean ± standard deviation reduction in cortisol levels of 50 ± 17% at study completion. Decreases in pre-ACTH-stimulated cortisol concentrations were observed in some dogs although overall changes in pre-ACTH cortisol concentrations were not significant. The compound was well-tolerated and no serious drug-related adverse effects were reported. This study highlights the potential utility of naturally occurring canine Cushing's syndrome as a model for human disease and provides proof of

  20. Analysis of H2O in silicate glass using attenuated total reflectance (ATR) micro-FTIR spectroscopy

    Science.gov (United States)

    Lowenstern, Jacob B.; Pitcher, Bradley W.

    2013-01-01

    We present a calibration for attenuated total reflectance (ATR) micro-FTIR for analysis of H2O in hydrous glass. A Ge ATR accessory was used to measure evanescent wave absorption by H2O within hydrous rhyolite and other standards. Absorbance at 3450 cm−1 (representing total H2O or H2Ot) and 1630 cm−1 (molecular H2O or H2Om) showed high correlation with measured H2O in the glasses as determined by transmission FTIR spectroscopy and manometry. For rhyolite, wt%H2O=245(±9)×A3450-0.22(±0.03) and wt%H2Om=235(±11)×A1630-0.20(±0.03) where A3450 and A1630 represent the ATR absorption at the relevant infrared wavelengths. The calibration permits determination of volatiles in singly polished glass samples with spot size down to ~5 μm (for H2O-rich samples) and detection limits of ~0.1 wt% H2O. Basaltic, basaltic andesite and dacitic glasses of known H2O concentrations fall along a density-adjusted calibration, indicating that ATR is relatively insensitive to glass composition, at least for calc-alkaline glasses. The following equation allows quantification of H2O in silicate glasses that range in composition from basalt to rhyolite: wt%H2O=(ω×A3450/ρ)+b where ω = 550 ± 21, b = −0.19 ± 0.03, ρ = density, in g/cm3, and A3450 is the ATR absorbance at 3450 cm−1. The ATR micro-FTIR technique is less sensitive than transmission FTIR, but requires only a singly polished sample for quantitative results, thus minimizing time for sample preparation. Compared with specular reflectance, it is more sensitive and better suited for imaging of H2O variations in heterogeneous samples such as melt inclusions. One drawback is that the technique can damage fragile samples and we therefore recommend mounting of unknowns in epoxy prior to polishing. Our calibration should hold for any Ge ATR crystals with the same incident angle (31°). Use of a different crystal type or geometry would require measurement of several H2O-bearing standards to provide a crystal

  1. Study of consumer fireworks post-blast residues by ATR-FTIR.

    Science.gov (United States)

    Martín-Alberca, Carlos; Zapata, Félix; Carrascosa, Héctor; Ortega-Ojeda, Fernando E; García-Ruiz, Carmen

    2016-03-01

    Specific analytical procedures are requested for the forensic analysis of pre- and post-blast consumer firework samples, which present significant challenges. Up to date, vibrational spectroscopic techniques such as Fourier transform infrared spectroscopy (FTIR) have not been tested for the analysis of post-blast residues in spite of their interesting strengths for the forensic field. Therefore, this work proposes a simple and fast procedure for the sampling and analysis of consumer firework post-blast residues by a portable FTIR instrument with an Attenuated Total Reflection (ATR) accessory. In addition, the post-blast residues spectra of several consumer fireworks were studied in order to achieve the identification of their original chemical compositions. Hence, this work analysed 22 standard reagents usually employed to make consumer fireworks, or because they are related to their combustion products. Then, 5 different consumer fireworks were exploded, and their residues were sampled with dry cotton swabs and directly analysed by ATR-FTIR. In addition, their pre-blast fuses and charges were also analysed in order to stablish a proper comparison. As a result, the identification of the original chemical compositions of the post-blast samples was obtained. Some of the compounds found were potassium chlorate, barium nitrate, potassium nitrate, potassium perchlorate or charcoal. An additional study involving chemometric tools found that the results might greatly depend on the swab head type used for the sampling, and its sampling efficiency. The proposed procedure could be used as a complementary technique for the analysis of consumer fireworks post-blast residues. Copyright © 2015 Elsevier B.V. All rights reserved.

  2. Cutaneous approach towards clinical and pathophysiological aspects of hyperglycemia by ATR FTIR spectroscopy

    Science.gov (United States)

    Eikje, Natalja Skrebova; Sota, Takayuki; Aizawa, Katsuo

    2007-07-01

    Attempts were made to non-invasively detect glucose-specific spectral signals in the skin by ATR-FTIR spectroscopy. In vivo spectra were collected from the inner wrists of healthy, prediabetes and diabetes subjects in the 750-4000 cm -1 region, with a closer assessment of the glucose-related region between 1000 and 1180 cm -1. Spectra in vivo showed glucose-specific peaks at 1030, 1080, 1118 and 1151 cm -1, as a variety of glucose solutions are found in vitro. Based on the differences of intensities at 1030 and 1118 cm -1 two spectral patterns were seen: I 1118 > I 1030 for a diabetes and I 1030> I 1118 for non-diabetes subjects. The peak at 1030 cm -1 was used to assess glucose concentrations in the skin due to its good correlation with glucose concentrations in vitro. Calculated mean values of the peak at 1030 cm -1 showed evidence of correlation with blood glucose levels when grouped as = 200 mg/dL, though there was no constant correlation between them when compared before/after OGTT or at the fasting/postprandial states. Absorbances at 1030 cm -1 were not only increased in a dose-dependent manner in a diabetes patient, but were also generally higher than in non-diabetes subjects at 30 min OGTT assessment. Also we could monitor absorbances at 1030 cm -1 and determine their changes in the skin tissue at different times of OGTT. We assume that our approach to in vivo measurement and monitoring of glucose concentrations at 1030 cm -1 may be one of the indicators to assess glucose activity level and its changes in the skin tissue, and has further implications in the study of clinical and pathophysiological aspects of hyperglycemia in diabetes and non-diabetes subjects by ATR-FTIR spectroscopy.

  3. Rapid detection of NBOME's and other NPS on blotter papers by direct ATR-FTIR spectrometry.

    Science.gov (United States)

    Coelho Neto, José

    2015-07-01

    Blotter paper is among the most common forms of consumption of new psychotropic substances (NPS), formerly referred as designer drugs. In many cases, users are misled to believe they are taking LSD when, in fact, they are taking newer and less known drugs like the NBOMEs or other substituted phenethylamines. We report our findings in quick testing of blotter papers for illicit substances like NBOMEs and other NPS by taking ATR-FTIR spectra directly from blotters seized on the streets, without any sample preparation. Both sides (front and back) of each blotter were tested. Collected data were analyzed by single- and multi-component spectral matching and submitted to chemometric discriminant analysis. Our results showed that, on 66.7% of the cases analyzed, seized blotters contained one or more types of NBOMEs, confirming the growing presence of this novel substances on the market. Matching IR signals were detected on both or just one side of the blotters and showed variable strength. Although no quantitative analysis was made, detection of these substances by the proposed approach serves as indication of variable and possibly higher dosages per blotter when compared to LSD, which showed to be below the detection limit of the applied method. Blotters containing a mescaline-like compound, later confirmed by GC-MS and LC-MS to be MAL (methallylescaline), a substance very similar to mescaline, were detected among the samples tested. Validity of direct ATR-FTIR testing was confirmed by checking the obtained results against independent GC-MS or LC-MS results for the same cases/samples. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  4. Rapid quantification of TBP and TBP degradation product ratios by FTIR-ATR

    International Nuclear Information System (INIS)

    Gillens, A.R.; Powell, B.A.; Clemson University, Clemson, SC

    2013-01-01

    Tri-n-butyl phosphate (TBP) is the key complexant within the plutonium and uranium reduction extraction process used to extract uranium and plutonium from used nuclear fuel. During reprocessing TBP degrades to dibutyl phosphate (DBP), butyl acid phosphate (MBP), butanol, and phosphoric acid over time. A method for rapidly monitoring TBP degradation is needed for the support of nuclear forensics. Therefore, a Fourier transform infrared spectrometry-attenuated total reflectance (FTIR-ATR) technique was developed to determine approximate peak intensity ratios of TBP and its degradation products. The technique was developed by combining variable concentrations of TBP, DBP, and MBP to simulate TBP degradation. This method is achieved by analyzing selected peak positions and peak intensity ratios of TBP and DBP at different stages of degradation. The developed technique was tested on TBP samples degraded with nitric acid. In mock degradation samples, the 1,235 cm -1 peak position shifts to 1,220 cm -1 as the concentration of TBP decreases and DBP increases. Peak intensity ratios of TBP positions at 1,279 and 1,020 cm -1 relative to DBP positions at 909 and 1,003 cm -1 demonstrate an increasing trend as the concentration of DBP increases. The same peak intensity ratios were used to analyze DBP relative to MBP whereas a decreasing trend is seen with increasing DBP concentrations. The technique developed from this study may be used as a tool to determine TBP degradation in nuclear reprocessing via a rapid FTIR-ATR measurement without gas chromatography analysis. (author)

  5. ATR technique, an appropriate method for determining the degree of conversion in dental giomers

    International Nuclear Information System (INIS)

    Prejmerean, Cristina; Prodan, Doina; Vlassa, Mihaela; Prejmerean, Vasile; Cuc, Stanca; Moldovan, Marioara; Streza, Mihaela; Buruiana, Tinca; Colceriu, Loredana

    2016-01-01

    Dental light-curing giomers were developed to combine the favourable properties of diacrylic resin composites (DRCs) and glass-ionomer cements (GICs) in a single material and to eliminate their inherent drawbacks. Giomers are characterized by their aesthetic appearance, high mechanical properties, adhesion to dental tissues as well as fluoride release and recharge abilities. The qualities of the giomers are greatly influenced by the level of conversion of the component resins. Infrared spectroscopy is one of the most largely used techniques for the determination of the degree of conversion in resin-based dental materials. However different results were obtained due to the performances of the used methods. The present work presents the determination of conversion degree in a series of dental copolymers and their corresponding giomers using transmission Fourier transform infrared spectroscopy (FTIR) and an attenuated total reflection technique (ATR) technique, respectively, the main aim being the study of the influence of the materials composition and of the light curing modes upon the achieved conversion in the cured giomers. Beautifil II commercial giomer was used as a control. A halogen lamp and a diode-blue LED lamp were used for the curing of the materials. The results showed that the composition of the resins greatly influenced the conversion. The highest conversions (up to 79%) were obtained in the case of the experimental giomers which contained the experimental Bis-GMA urethane analogue, followed by the Beautifil II giomer (61%) and experimental giomers based on commercial Bis-GMA (up to 50%), respectively. The resins light-cured by using the diode-blue LED lamp presented slightly higher conversions than the resins cured by halogen lamp. The study demonstrates the possibility to evaluate easily and reproducibly the conversion in light-curing composite materials with complex chemical composition and structure, particularly in the case of giomers by using the

  6. Differential Processing of Low and High LET Radiation Induced DNA Damage: Investigation of Switch from ATM to ATR Signaling

    Science.gov (United States)

    Saha, Janapriya; Wang, Minli; Hada, Megumi; Cucinotta, Francis A.

    2011-01-01

    The members of the phosphatidylinositol kinase-like kinase family of proteins namely ataxia-telangiectasia mutated (ATM) and ATM- and Rad3-related (ATR) are directly responsible for the maintenance of genomic integrity by mounting DDR through signaling and facilitating the recruitment of repair factors at the sites of DNA damage along with coordinating the deployment of cell cycle checkpoints to permit repair by phosphorylating Checkpoint kinase Chk1, Chk2 and p53. High LET radiation from GCR (Galactic Cosmic Rays) consisting mainly of protons and high energy and charged (HZE) particles from SPE (Solar Particle Event) pose a major health risk for astronauts on their space flight missions. The determination of these risks and the design of potential safeguards require sound knowledge of the biological consequences of lesion induction and the capability of the cells to counter them. We here strive to determine the coordination of ATM and ATR kinases at the break sites directly affecting checkpoint signaling and DNA repair and whether differential processing of breaks induced by low and high LET radiation leads to possible augmentation of swap of these damage sensors at the sites of DNA damage. Exposure of cells to IR triggers rapid autophosphorylation of serine-1981 that causes dimer dissociation and initiates monomer formation of ATM. ATM kinase activity depends on the disruption of the dimer, which allows access and phosphorylation of downstream ATM substrates like Chk2. Evidence suggests that ATM is activated by the alterations in higher-order chromatin structure although direct binding of ATM to DSB ends may be a crucial step in its activation. On the other hand, in case of ATR, RPA (replication protein A)-coated ssDNA (single-stranded DNA) generated as a result of stalled DNA replication or during processing of chromosomal lesions is crucial for the localization of ATR to sites of DNA damage in association with ATR-interacting protein (ATRIP). Although the

  7. Identifikasi Carrier Bakteri Streptococcus β hemolyticus Group A pada Murid SD Negeri 13 Padang Berdasarkan Perbedaan Umur dan Jenis Kelamin

    Directory of Open Access Journals (Sweden)

    Fadhila Aini

    2016-01-01

    Full Text Available AbstrakStreptococcus β hemolyticus Grup A atau yang disebut juga Streptococcus pyogenes merupakan salah satu bakteri patogen yang banyak menginfeksi manusia.Bakteri ini dapat ditemukan sebagai carrier di saluran pernafasan terutama pada anak-anak, tidak menimbulkan penyakit tetapi berisiko untuk menyebarkan penyakit. Tujuan penelitian ini adalah menentukan jumlah carrier  bakteri Streptococcus β hemolyticus Grup A pada murid berdasarkan perbedaan umur dan jenis kelamin. Jenis penelitian ini adalah deskriptif cross-sectional dengan menggunakan sampel seluruh murid SD Negeri 13 Padang. Hasil penelitian adalah didapatkan 2 orang murid yang menderita carrier, yaitu pada kelompok usia>8-9 tahun dan >11 tahun. Berdasarkan jenis kelamin yang terdiri dari 54 orang laki-laki dan 50 orang perempuan, didapatkan 2 orang carrier yaitu hanya pada anak laki-laki. Hasil penelitian menunjukkan bahwa carrier bakteri Streptococcus β hemolyticus Group  A terdapat pada anak usia tersebut karena masih kurangnya pengetahuan tentang kebersihan. Carrier yang ditemukan hanya pada anak laki-laki kemungkinan disebabkan mereka lebih sering bermain di luar rumah dan terpapar dengan berbagai bakteri patogen dan kurang memperhatikan kebersihan diri.Kata kunci: carrier, streptococcus β hemolyticus grup A, umur, jenis kelamin AbstractGroup A Streptococcus β hemolyticus or also called Streptococcus pyogenes is one of many pathogenic bacteria that infect humans. These bacteria can be found as a carrier in the respiratory tract especially in children, do not cause disease but can be a risk for spreading the disease. This objective of this study was to determine the amount of the carrier of bacteria group A Streptococcus β hemolyticus based on age and gender differences. This research is a descriptive cross - sectional study using a sample of all students of SD Negeri 13 Padang. Based on the age of 104 students found that students who suffer 2 carrier, which is in the age

  8. Hubungan Keteraturan Penggunaan Kortikosteroid Inhalasi dengan Tingkat Kontrol Asma Pasien Berdasarkan ACT di Poliklinik Paru RSUP Dr. M. Djamil Padang

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    Wulan Prisilla Prisilla

    2016-01-01

    Full Text Available AbstrakAsma merupakan penyakit yang didasari oleh reaksi inflamasi pada saluran napas yang dapat dicegah dengan kortikosteroid inhalasi. Asma sukar disembuhkan, sehingga tujuan penatalaksanaan asma adalah asma terkontrol. Penilaian tingkat kontrol asma dapat dilakukan dengan menggunakan kuesioner Asthma Control Test  (ACT yang bersifat subjektif tetapi validitasnya telah diuji. Tujuan penelitian ini adalah menentukan hubungan keteraturan penggunaan kortikosteroid inhalasi dengan tingkat kontrol asma pasien berdasarkan ACT di Poliklinik Paru RSUP Dr. M. Djamil Padang. Penelitian cross sectional analytic ini telah dilakukan dari September hingga Desember 2013. Populasi adalah pasien berusia ≥14 tahun yang didiagnosis asma oleh dokter. Jumlah subjek penelitian sebanyak 96 orang. Data yang diperoleh diolah dan dianalisis menggunakan uji Kolmogorov-Smirnov. Insiden terbanyak asma berada pada kelompok usia lanjut (60,4%. Sebagian besar pasien tidak teratur menggunakan kortikosteroid inhalasi (63,5% dan pasien dengan asma tidak terkontrol memiliki proporsi tertinggi (59,4% . Analisis statistik menunjukkan bahwa terdapat hubungan yang bermakna antara keteraturan penggunaan kortikosteroid inhalasi dengan tingkat kontrol asma pasien berdasarkan ACT di Poliklinik Paru RSUP Dr. M. Djamil (p=0,002.Kata kunci: kortikosteroid inhalasi, ACT, tingkat control asma AbstractAsthma is a disease caused by an inflammatory reaction in the patient airways that can be prevented with inhaled corticosteroids. It is known that there is no cure for asthma, so the goal of asthma management is to obtain controlled asthma. The level of asthma control can be assessed by  using Asthma Control Test  (ACT questionnaire, this method is subjective but its validity has been tested. The objective of this study  was to determine the association between regularity of inhaled corticosteroids application and patient’s level of asthma control based on ACT in the Lung Clinic of Dr

  9. Segmentasi Citra menggunakan Support Vector Machine (SVM dan Ellipsoid Region Search Strategy (ERSS Arimoto Entropy berdasarkan Ciri Warna dan Tekstur

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    Lukman Hakim

    2016-02-01

    Full Text Available Abstrak Segmentasi citra merupakan suatu metode penting dalam pengolahan citra digital yang bertujuan membagi citra menjadi beberapa region yang homogen berdasarkan kriteria kemiripan tertentu. Salah satu syarat utama yang harus dimiliki suatu metode segmentasi citra yaitu menghasilkan citra boundary yang optimal.Untuk memenuhi syarat tersebut suatu metode segmentasi membutuhkan suatu klasifikasi piksel citra yang dapat memisahkan piksel secara linier dan non-linear. Pada penelitian ini, penulis mengusulkan metode segmentasi citra menggunakan SVM dan entropi Arimoto berbasis ERSS sehingga tahan terhadap derau dan mempunyai kompleksitas yang rendah untuk menghasilkan citra boundary yang optimal. Pertama, ekstraksi ciri warna dengan local homogeneity dan ciri tekstur dengan menggunakan Gray Level Co-occurrence Matrix (GLCM yang menghasilkan beberapa fitur. Kedua, pelabelan dengan Arimoto berbasis ERSS yang digunakan sebagai kelas dalam klasifikasi. Ketiga, hasil ekstraksi fitur dan training kemudian diklasifikasi berdasarkan label dengan SVM yang telah di-training. Dari percobaan yang dilakukan menunjukkan hasil segmentasi kurang optimal dengan akurasi 69 %. Reduksi fitur perlu dilakukan untuk menghasilkan citra yang tersegmentasi dengan baik. Kata kunci: segmentasi citra, support vector machine, ERSS Arimoto Entropy, ekstraksi ciri. Abstract Image segmentation is an important tool in image processing that divides an image into homogeneous regions based on certain similarity criteria, which ideally should be meaning-full for a certain purpose. Optimal boundary is one of the main criteria that an image segmentation method should has. A classification method that can partitions pixel linearly or non-linearly is needed by an image segmentation method. We propose a color image segmentation using Support Vector Machine (SVM classification and ERSS Arimoto entropy thresholding to get optimal boundary of segmented image that noise-free and low complexity

  10. ANALISIS KEMAMPUAN PEMECAHAN MASALAH MATEMATIKA SOAL SETIPE TIMSS BERDASARKAN GAYA KOGNITIF SISWA PADA PEMBELAJARAN MODEL PROBLEM BASED LEARNING

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    Lia Vendiagrys

    2015-08-01

    Full Text Available Gaya kognitif memiliki peran yang sangat penting dalam proses pemecahan masalah. Penelitian ini bertujuan untuk memperoleh deskripsi profil kemampuan pemecahan masalah  matematika soal setipe TIMSS pada siswa SMP kelas VIII dengan gaya kognitif FI dan FD. Penelitian ini merupakan penelitian deskriptif. Subjek penelitian ini adalah tiga siswa FI dan tiga siswa FD kelas VIII SMP 3 Kudus. Teknik pengumpulan data adalah dokumen, tes, dan wawancara. Analisis data meliputi reduksi, penyajian data,dan  penarikan kesimpulan. Hasil penelitian sebagai berikut (1 untuk subjek FI dalam  menyelesaikan masalah memiliki profil:  dapat memahami pernyataan verbal dari masalah dan mengubahnya ke dalam kalimat matematika, lebih analitis dalam menerima informasi,  dapat memperluas hasil pemecahan masalah dan pemikiran matematis, memberikan suatu pembenaran berdasarkan pada hasil,dan memecahkan masalah dalam konteks kehidupan nyata,  memperoleh jawaban yang benar, (2  Untuk subjek FD dalam menyelesaikan masalah memiliki profil: dapat memahami pernyataan verbal dari masalah,tetapi tidak dapat mengubahnya ke dalam kalimat matematika,  lebih global dalam menerima informasi, mudah terpengaruh manipulasi unsur pengecoh karena memandang secara global, tidak dapat memperluas hasil pemecahan masalah, memberikan suatu pembenaran berdasarkan pada hasil,dan memecahkan masalah dalam konteks kehidupan nyata, sering tidak dapat memperoleh jawaban yang benar.Cognitive style has a very important role in the process of problem solving. This study aimed to obtain a profile of mathematical problem solving ability student with FI and FD cognitive style. This research is a descriptive qualitative approach. Subjects in this study were students of class VIII SMP 3 Kudus, ie three students FI and FD. Data collection techniques is a document, test, interview. Data analysis included reduction, presentation, and conclusion. The results of the study show that subject FI in resolving

  11. Perancangan SMKI Berdasarkan SNI ISO/IEC27001:2013 dan SNI ISO/IEC27005:2013 (Studi Kasus DPTSI-ITS

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    Furqon Mauladani

    2018-03-01

    Full Text Available Institut Teknologi Sepuluh Nopember (ITS adalah salah satu universitas di Surabaya. ITS telah menggunakan TIK untuk keperluan operasional bisnisnya (contohnya isi kartu program studi, proses absensi, pembaharuan informasi, dan lainnya. Penggunaan TIK tidak dapat dipisahkan dari ancaman yang dapat mengganggu operasional TIK. Ancaman terdiri dari ancaman yang berasal dari luar (penyebaran malware, aktifitas social engineering, orang dalam (sengaja, tidak sengaja, kegagalan teknis (kesalahan penggunaan, kegagalan perangkat keras/lunak ataupun bencana alam (kebakaran, gempa, banjir. Metode yang digunakan pada penelitian ini adalah melakukan manajemen resiko keamanan informasi berdasarkan SNI ISO/IEC 27005 dan perancangan dokumen SMKI berdasarkan SNI ISO/IEC 27001. Hasil penelitian ini adalah 60 resiko yang tidak diterima dari total 228 resiko yang telah teridentifikasi. Dari 60 resiko tersebut, terdapat 58 risk modification, 1 risk avoidance, dan 1 risk sharing. Tata kelola keamanan informasi yang dirancang berdasarkan SNI ISO/IEC 27001 adalah ruang lingkup SMKI, kebijakan SMKI, proses penilaian resiko, proses penanganan resiko, statement of applicability, dan sasaran keamanan informasi.

  12. Status Report on the Fabrication of Fuel Cladding Chemical Interaction Test Articles for ATR Irradiations

    Energy Technology Data Exchange (ETDEWEB)

    Field, Kevin G. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Howard, Richard H. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2015-09-28

    FeCrAl alloys are a promising new class of alloys for light water reactor (LWR) applications due to their superior oxidation and corrosion resistance in high temperature environments. The current R&D efforts have focused on the alloy composition and processing routes to generate nuclear grade FeCrAl alloys with optimized properties for enhanced accident tolerance while maintaining properties needed for normal operation conditions. Therefore, the composition and processing routes must be optimized to maintain the high temperature steam oxidation (typically achieved by increasing the Cr and Al content) while still exhibiting properties conducive to normal operation in a LWR (such as radiation tolerance where reducing Cr content is favorable). Within this balancing act is the addition of understanding the influence on composition and processing routes on the FeCrAl alloys for fuel-cladding chemical interactions (FCCI). Currently, limited knowledge exists on FCCI for the FeCrAl-UO2 clad-fuel system. To overcome the knowledge gaps on the FCCI for the FeCrAl-UO2 clad-fuel system a series of fueled irradiation tests have been developed for irradiation in the Advanced Test Reactor (ATR) housed at the Idaho National Laboratory (INL). The first series of tests has already been reported. These tests used miniaturized 17x17 PWR fuel geometry rodlets of second-generation FeCrAl alloys fueled with industrial Westinghouse UO2 fuel. These rodlets were encapsulated within a stainless steel housing.To provide high fidelity experiments and more robust testing, a new series of rodlets have been developed deemed the Accident Tolerant Fuel Experiment #1 Oak Ridge National Laboratory FCCI test (ATF-1 ORNL FCCI). The main driving factor, which is discussed in detail, was to provide a radiation environment where prototypical fuel-clad interface temperatures are met while still maintaining constant contact between industrial fuel and the candidate cladding alloys

  13. Penanganan Teror Bom Thamrin Ditinjau Berdasarkan Skep Kapolri Nomor: 172 Tahun 2014 tentang Penanganan Tempat Kejadian Perkara (PTKP Bom

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    Dedy Tabrani

    2016-11-01

    Full Text Available Abstrak Fenomena ISIS pasca Al-Qaeda telah memberikan harapan baru dan semangat baru kelompok radikal Islam di Indonesia. Salah satu aksi terorisme yang dilakukan oleh anggota ISIS di Indonesia adalah terror Bom Thamrin pada 14 Januari 2016 dengan pola dan modus berupa bom dan penembakan. Polri sebagai leading sector penanganan terorisme, telah merumuskan beberapa peraturan mengenai penanganan terorisme, misalnya, Keputusan Kepala Kepolisian Negara Republik Indonesia Nomor: 172 Tahun 2014 tentang Penanganan Tempat Kejadian Perkara Bom (PTKP. Keputusan tersebut menjadi landasan kepolisian dalam penanganan kasus teror di lapangan. Untuk itu, penelitian ini akan mengungkap sejauh mana penanganan terror bom Thamrin ditinjau berdasarkan Skep Kapolri Nomor: 172 Tahun 2014 Tentang Penanganan Tempat Kejadian Perkara Bom (PTKP. Kata kunci: terorisme, Teror Thamrin, ISIS, Polri   ISIS phenomena after Al-Qaeda have given new hope and spirit of Islamic radicalism groups in Indonesia. One of terrorism action by ISIS members was Thamrin bombing terror on January 14, 2016 with pattern and modus of bombings and shootings. Indonesian National Police as a leading sector in handling terrorism has formulated rules regarding terrorism handling, such as the Decree of the Indonesian National Police Chief (Perkap No. 172, 2014 on the Handling of the Crime Scene (PTKP of bomb. This decree becomes the base of police on handling terror scene. Therefore, this research can reveal how far the handling of Thamrin terror bombing based on Skep No. 173, 2014. Keywords: Terrorism, Terror of Thamrin, ISIS, Indonesian National Police

  14. PENGAWASAN, EVALUASI, DAN PENILAIAN SISTEM PENGENDALIAN INTERNAL TERKAIT BILLING SYSTEM PADA RSUD UNGARAN BERDASARKAN KERANGKA KERJA COBIT 5 (MEA02

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    Marthania Firmadita

    2016-10-01

    Full Text Available RSUD Ungaran Kabupaten Semarang ini memiliki sejumlah layanan kesehatan yang melayani masyarakat umum, layanan kesehatan dinamakan dengan Billing System yang dibuat dengan tujuan mempercepat pelayanan kesehatan terhadap pelanggan. Melihat banyaknya kebutuhan setiap bagian yang terkait sehingga semakin besar pengelolaan data yang ada menyebabkan keterlambatan sistem dan kesalahan dalam pengelolaan data. Berbagai upaya dilakukan untuk memberikan layanan operasional terbaik dan meminimalisasi kesalahan. Salah satunya dilakukan penilaian kinerja pengelolaan layanan yang diberikan, penilaian kinerja ini merupakan pengendalian kontrol internal secara sistematik, untuk melihat tingkat kesesuaian dan keefektifan implementasi layanan. Dari hasil observasi, wawancara, dan kuesioner berdasarkan COBIT 5 dihasilkan level kapabilitas tata kelola proses pengawasan, evaluasi, dan penilaian pengendalian internal terkait billing system (MEA02 pada RSUD Ungaran saat ini adalah Level 2 dengan status Largely Achieved sebesar 71,56% setara 2,71 dimana Level 1 dan Level 0 tercapai Fully Achieved.Hal ini menunjukan sudah dilakukan komunikasi perencanaan kedepan dari hasil penilaian kinerja tetapi belum diimplementasikan. Dan untuk mencapai level kapabilitas, RSUD Ungaran dapat melakukan strategi perbaikan yang dilakukan secara bertahap dari proses atribut level 1 sampai 3. Kata Kunci: Analisis Tata Kelola TI, Sistem Informasi Kesehatan, COBIT 5, Analisis Tingkat Kapabilitas, Analisis Kesenjangan

  15. GAMBARAN RISIKO TROMBOSIS VENA PROFUNDA (TVP PADA SALES PROMOTION GIRL (SPG BERDASARKAN KRITERIA WELLS DI KOTA DENPASAR

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    Shelly Silvia Bintang

    2015-01-01

    Full Text Available Pekerjaan sebagai sales promotion girl (SPG menuntut berdiri dalam posisi statis dalam waktu kerja yang cukup lama. Dalam sebuah jurnal kesehatan masyarakat tahun 2013 mendapatkan hasil semakin lama SPG bekerja maka semakin lama juga durasi mereka memakai sepatu hak tinggi semakin besar risiko untuk mengalami gangguan kesehatan yang disebabkan sepatu hak tinggi. Gangguan dari aliran darah atau trombosis vena profunda (TVP merupakan dampak yang ditimbulkan. Tujuan dari penelitian ini untuk mengetahui angka tinggi pemakaian sepatu hak tinggi, angka pemakaian stocking dan gambaran SPG di Kota Denpasar yang memiliki risiko rendah, menengah dan tinggi mengalami TVP. Penelitian ini menggunakan metode deskriptif potong lintang. Subyek penelitian ini adalah 62 pegawai SPG yang bekerja di swalayan di Kota Denpasar. Skor kriteria Wells didapatkan untuk mengetahui gambaran risiko probabilitas TVP. Hasil gambaran risiko TVP pada SPG di Kota Denpasar berdasarkan kriteria Wells didapatkan 30 orang pekerja (48,38% menunjukan risiko ringan, dan 30 orang pekerja lainnya (48,38% menunjukan risiko menengah menderita TVP, sedangkan 2 orang pekerja (3,24% menunjukan risiko tinggi. Sebagian besar pegawai SPG di kota Denpasar memiliki risiko rendah dan sedang probabilitas TVP.

  16. Identifikasi Tumbuhan Obat Herbal Berdasarkan Citra Daun Menggunakan Algoritma Gray Level Co-occurence Matrix dan K-Nearest Neighbor

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    Fittria Shofrotun Ni'mah

    2018-03-01

    Full Text Available Medicinal plants can be used as an alternative natural treatment, instead of chemical drugs. But because of too many types of plants and lack of knowledge, it will be difficult to identify these herbs. Computer assistance can be used to facilitate the identification of these herbs. This research proposes the identification of herbal plants based on leaf image using texture analysis. There are 10 types of herbal medicinal plants used in this study. The texture analysis used was GLCM by extracting contrast, correlation, energy, and homogeneity. Classification is done by KNN. The result of the experiment showed that the accuracy of identification using 9-fold cross-cross validation method was 83.33% using 9 subsets. Tumbuhan obat herbal bisa dijadikan sebagai alternatif pengobatan yang alami, selain obat-obatan kimia. Namun karena terlalu banyak jenis tumbuhan dan kurangnya pengetahuan, identifikasi tumbuhan berkhasiat akan sulit. Bantuan komputer dapat digunakan untuk memudahkan mengidentifikasi tumbuhan herbal tersebut. Penelitian ini mengusulkan identifikasi tumbuhan herbal berdasarkan citra daun menggunakan analisis tekstur. Ada 10 spesies tumbuhan obat herbal yang digunakan dalam penelitian ini. Analisis tekstur yang digunakan adalah GLCM dengan mengekstrak nilai kontras, korelasi, energi dan homogenitas. Klasifikasi dilakukan dengan KNN. Hasil percobaan menunjukkan akurasi identifikasi menggunakan metode 9-fold cross validation mencapai 83.33% dengan menggunakan 9 subset.

  17. PEMODELAN STRATEGI PEMASARAN PRODUK BARANG JADI TEKSTIL BERDASARKAN PENDEKATAN SIMULASI SISTEM DINAMIK PADA INDUSTRI KECIL MENENGAH DI KOTA BOGOR

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    Nunung Nurhasanah

    2012-10-01

    Full Text Available Pertumbuhan Industri Kecil Menengah (IKM hingga saat ini terus meningkat, berdasarkan data dari Departemen Perindustrian (2004 dinyatakan jumlah IKM pada tahun 2002 adalah 2,55 juta unit usaha dan pada tahun 2003 meningkat menjadi 3 juta unit usaha. Berarti telah terjadi laju pertumbuhan IKM sebesar 15% pertahun. Sehingga hal ini menunjukkan tingginya persaingan yang terjadi di antara IKM. Kemampuan IKM dalam persaingan di dunia industri tidak terlepas dari  kemampuan penerapan strategi pemasaran yang digunakan. Strategi pemasaran  merupakan salah satu kunci keberhasilan IKM dalam meningkatkan jumlah penjualan produknya. Berdasarkan data dari Departemen Perindustrian (2004 diketahui bahwa IKM produk  barang jadi tekstil memiliki laju pertumbuhan  ekspor tertinggi, yaitu sebesar 23,49% pertahun. Hal ini menunjukkan bahwa IKM  produk barang jadi tekstil merupakan suatu industri yang potensial untuk terus dijaga keberlangsungan hidupnya. Akan tetapi yang menjadi permasalahan IKM produk  barang jadi tekstil adalah lemahnya kekontinyuan keberadaan industri tersebut.  Beberapa industri yang muncul tidak mempunyai siklus hidup bisnis yang lama, salah satu faktor penyebabnya adalah ketidak mampuan IKM dalam meningkatkan  penjualan karena lemahnya strategi pemasaran. Berdasarkan hal-hal tersebut diatas, maka penelitian tentang pemodelan strategi pemasaran untuk IKM sangat penting untuk dilakukan. Penelitian  ini bertujuan untuk  menentukan kebijakan  strategi  pemasaran produk barang jadi tekstil Industri Kecil Menengah di Kota Bogor  berdasarkan  pendekatan simulasi sistem dinamik yang dibantu oleh perangkat lunak Power Simulation. Pengolahan data dilakukan dengan memetakan posisi IKM  dengan menggunakan pendekatan Matriks BCG (Boston Consulting Group dimana posisi IKM sebagai obyek penelitian tersebut akan terpetakan kedalam posisi   Bintang (Star, Sapi Perah (Cash  Cow, Tanda tanya (Question Mark dan Anjing  (Dogs. Sebelumnya

  18. Keragaman suweg (Amorphophallus campanulatus di wilayah eks karesidenan Surakarta berdasarkan karakter morfologi, anatomi dan pola pita isozim

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    MONARITA PERMATASARI

    2014-05-01

    Full Text Available Permatasari, Pitoyo A, Suratman. 2014. Keragaman suweg (Amorphophallus campanulatus di wilayah eks karesidenan Surakarta berdasarkan karakter morfologi, anatomi dan pola pita isozim. Bioteknologi 11: 11-18. The aims of this research were to find out the diversity and relationship among elephant yam in Surakarta region based on variation of morphological, anatomical and isozyme banding patterns characters. Total 20 samples were taken using random purposive sampling technique in different altitude of 7 districts. Morphological, anatomical and isozyme banding pattern data were analyzed descriptively. The steady data of quantitative characteristic from morphological, anatomical and isozyme banding pattern of binary data was analyzed using an Index of Similarity (IS and computed by the program Numerical Taxonomy and Multivariate Analysis System (NTSYS version 2.10. The result showed that the elephant yam in Surakarta region were diverse based on morphological, anatomical and isozyme banding pattern, especially in ratio and color of petiolus; tuber ratio; index of stomatal; thick epidermis; thick mesophyll; term and ratio of palisade; term rafida; 4 peroxidase and 5 esterase isozyme banding pattern. Analysis of relationship of elephant yam based on morphological, anatomical and isozyme band pattern characters showed the similarity coefficient of 64,85% contained two major group. The first group consist of Boyolali 1, Boyolali 3, Boyolali 4, Wonogiri 4, Wonogiri 2, Karanganyar 1, Karanganyar 2, Karanganyar 3, Sragen 2, while the other samples were separated from this group.

  19. Kajian Investasi Pembangunan Jalan Tol di Indonesia Berdasarkan Sistem Syariah: Studi Kasus Jalan Tol Cikampek-Palimanan

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    Rudy Hermawan Karsaman

    2015-05-01

    Full Text Available Abstrak. Sebagai alternatif investasi pembangunan jalan tol di Indonesia yang biasanya  menggunakan metode konvensional berupa penanaman modal biasa, studi ini menunjukkan bahwa investasi tersebut dapat juga dilakukan menggunakan metode syariah yang berdasarkan prinsip bagi hasil dari marjin keuntungan. Dengan menggunakan studi kasus  proyek pembangunan jalan tol Cikampek – Palimanan, studi ini menemukan bahwa secara finansial keuntungan atau kerugian metode syariah dibandingkan dengan metode konvensional tergantung pada besarnya margin keuntungan/bagi hasil dibanding dengan bunga bank yang diterapkan. Selain itu pembiayaan metode syariah  mensyaratkan adanya pembagian risiko bersama dan merupakan salah satu perbedaan antara metode syariah dan metode konvensional. Kata kunci. Investasi, sistem pembiayaan syariah, analisis kelayakan dan risiko, jalan tol Abstract. As an alternative of toll road development investment in Indonesia, which generally uses conventional methods,  this study shows that the investment can also uses sharia method of financing which was based on sharing principle. Using a case study of Cikampek – Palimanan toll road development project, it  revealed that financially the advantages or disadvantages of sharia method  compared with the conventional ones depend on the amount of profit margin or share profit compared with applied bank interest. Furthermore, the sharia method of financing involves risk sharing, which was one of the differences between the sharia method of financing and the conventional one.Keywords. Investment, sharia financing system, viability and risk analysis, toll road

  20. HUBUNGAN KEKERABATAN BEBERAPA POPULASI KERANG HIJAU (Perna viridis DI INDONESIA BERDASARKAN SEKUEN CYTROCROME B mtDNA

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    Achmad Sudradjat

    2016-11-01

    Full Text Available Penelitian ini dilakukan untuk mengetahui hubungan kekerabatan stok kerang hijau (Perna viridis di beberapa perairan Indonesia sebagai informasi dasar bagi program pemuliaan. Sampel kerang hijau yang berasal dari populasi alam perairan Tanjung Kait, Kamal, Panimbang, Cirebon, Pasuruan, Kenjeran, dan Pangkep diambil secara acak. Amplifikasi PCR dan sekuensing mitokondria daerah cytochrome B adalah HCO (F: 5’-TAA ACT TCA GGG TGA CCA AAA AAT CA-3’ (26 bp dan LCO (R: 5’-GGT CAA CAA ATC ATA AAG ATA TTG G-3’ (25 bp. Sekuen DNA yang diperoleh digunakan untuk analisis homologi, analisis genetic distance dan analisis kekerabatan. Hasil analisis homologi susunan nukleotida berdasarkan BLAST-N terhadap sekuen mtDNA Perna viridis yang tersimpan di Genebank menunjukkan similaritas 97%. Hasil analisis didapatkan jarak genetik yang terdekat adalah populasi Tanjung Kait dengan Kenjeran sedangkan jarak genetik terjauh adalah populasi Cirebon dengan Kamal. Hubungan kekerabatan yang ditunjukkan dengan dendrogram diperoleh 2 kelompok yaitu 6 populasi membentuk satu kelompok dan populasi Cirebon membentuk kluster tersendiri. Sekuens tersebut mungkin dapat digunakan sebagai penanda dalam program breeding kerang hijau di Indonesia

  1. Electrooxidation of ethanol on Pt and PtRu surfaces investigated by ATR surface-enhanced infrared absorption spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Marcio F.; Camara, Giuseppe A., E-mail: giuseppe.silva@ufms.br [Departamento de Quimica, Universidade Federal do Mato Grosso do Sul, Campo Grande-MS (Brazil); Batista, Bruno C.; Boscheto, Emerson [Instituto de Quimica de Sao Carlos, Universidade de Sao Paulo, Sao Carlos-SP, (Brazil); Varela, Hamilton, E-mail: varela@iqsc.usp.br [Ertl Center for Electrochemistry and Catalysis, Gwangju Institute of Science and Technology (GIST), Gwangju (Korea, Republic of)

    2012-05-15

    Herein, it was investigated for the first time the electro-oxidation of ethanol on Pt and PtRu electrodeposits in acidic media by using in situ surface enhanced infrared absorption spectroscopy with attenuated total reflection (ATR-SEIRAS). The experimental setup circumvents the weak absorbance signals related to adsorbed species, usually observed for rough, electrodeposited surfaces, and allows a full description of the CO coverage with the potential for both catalysts. The dynamics of adsorption-oxidation of CO was accessed by ATR-SEIRAS experiments (involving four ethanol concentrations) and correlated with expressions derived from a simple kinetic model. Kinetic analysis suggests that the growing of the CO adsorbed layer is nor influenced by the presence of Ru neither by the concentration of ethanol. The results suggest that the C-C scission is not related to the presence of Ru and probably happens at Pt sites. (author)

  2. ATR-FTIR Spectroscopy on intact dried leaves of sage (Salvia officinalis L. – chemotaxonomic discrimination and essential oil composition

    Directory of Open Access Journals (Sweden)

    Gudi, Gennadi

    2016-07-01

    Full Text Available Sage (Salvia officinalis L. is cultivated worldwide for its aromatic leaves which are used as herbal spice and for phytopharmaceutical applications. Fast analytical strategies for essential oil analysis, performed directly on plant material would reduce the delay between sampling and analytical results. This would enhance product quality by improving technical control of cultivation. The attenuated total reflectance Fourier transform infrared (ATR-FTIR spectroscopy method described here provides a reliable calibration model for quantification of essential oil components (EOC and its main constituents (e.g. -thujone and -thujone directly on dried, intact leaves of sage. Except for drying no further sample preparation is required for ATR-FTIR and the measurement time of less than 5 min per sample contrasts with the most common alternative of hydro-distillation followed by GC analysis which can take several hours per sample.

  3. AGR-2 Final Data Qualification Report for U.S. Capsules - ATR Cycles 147A Through 154B

    Energy Technology Data Exchange (ETDEWEB)

    Pham, Binh T. [Idaho National Lab. (INL), Idaho Falls, ID (United States). Very High-Temperature Reactor Technology Development Office; Einerson, Jeffrey J. [Idaho National Lab. (INL), Idaho Falls, ID (United States). Very High-Temperature Reactor Technology Development Office

    2014-07-01

    This report provides the data qualification status of AGR-2 fuel irradiation experimental data in four U.S. capsules from all 15 Advanced Test Reactor (ATR) Cycles 147A, 148A, 148B, 149A, 149B, 150A, 150B, 151A, 151B, 152A, 152B, 153A, 153B, 154A, and 154B, as recorded in the Nuclear Data Management and Analysis System (NDMAS). Thus, this report covers data qualification status for the entire AGR-2 irradiation and will replace four previously issued AGR-2 data qualification reports (e.g., INL/EXT-11-22798, INL/EXT-12-26184, INL/EXT-13-29701, and INL/EXT-13-30750). During AGR-2 irradiation, two cycles, 152A and 153A, occurred when the ATR core was briefly at low power, so AGR-2 irradiation data are not used for physics and thermal calculations. Also, two cycles, 150A and 153B, are Power Axial Locator Mechanism (PALM) cycles when the ATR power is higher than during normal cycles. During the first PALM cycle, 150A, the experiment was temporarily moved from the B-12 location to the ATR water canal and during the second PALM cycle, 153B, the experiment was temporarily moved from the B-12 location to the I-24 location to avoid being overheated. During the “Outage” cycle, 153A, seven flow meters were installed downstream from seven Fission Product Monitoring System (FPMS) monitors to measure flows from the monitors and these data are included in the NDMAS database.

  4. Raman and IR-ATR spectroscopy studies of heteroepitaxial structures with a GaN:C top layer

    Science.gov (United States)

    Cerqueira, M. F.; Vieira, L. G.; Alves, A.; Correia, R.; Huber, M.; Andreev, A.; Bonanni, A.; Vasilevskiy, M. I.

    2017-09-01

    This work, motivated by the technologically important task of determination of carbon dopant location in the GaN crystal lattice, employed Raman spectroscopy, with both resonant and non-resonant excitation, and infrared (IR) spectroscopy, in the attenuated total reflection (ATR) configuration, to study lattice vibration modes in a set of carbon-doped GaN (GaN:C) epilayers grown by metalorganic vapour phase epitaxy. We analyse Raman and IR-ATR spectra from the point of view of possible effects of the carbon doping, namely: (i) local vibration mode of C atoms in the nitrogen sublattice (whose frequency we theoretically estimate as 768 cm-1 using an isotope defect model), and (ii) shift in the positions of longitudinal modes owing to the phonon-plasmon coupling. We find only indirect hints of the doping effect on the resonant Raman spectra. However, we show theoretically and confirm experimentally that the IR-ATR spectroscopy can be a much more sensitive tool for this purpose, at least for the considered structures. A weak perturbation of the dielectric function of GaN:C, caused by the substitutional carbon impurity, is shown to produce a measurable dip in the ATR reflectivity spectra at  ≈770 cm-1 for both p- and s-polarizations. Moreover, it influences a specific (guided-wave type) mode observed at  ≈737 cm-1, originating from the GaN layer, which appears in the narrow frequency window where the real parts of the two components of the dielectric tensor of the hexagonal crystal have opposite signs. This interpretation is supported by our modelling of the whole multilayer structure, using a transfer matrix formalism.

  5. Determination of drug content in semisolid formulations by non-invasive spectroscopic methods: FTIR - ATR, - PAS, - Raman and PDS

    International Nuclear Information System (INIS)

    Gotter, B; Hein, J; Neubert, R H H; Faubel, W; Heissler, St

    2010-01-01

    This study elucidates the potential use of photothermal deflection spectroscopy (PDS), FTIR photoacoustic (FTIR-PAS), FT Raman, and FTIR-attenuated total reflection (FTIR-ATR) spectroscopy as analytical tools for investigating the drug content in semisolid formulations. Regarding the analytical parameters, this study demonstrates the photothermal beam deflection to be definitely comparable to well established spectroscopic methods for this purpose. The correlation coefficients range from 0.990 to 0.999. Likewise, repeatability and limit of detection are comparable.

  6. Analysis code for medium and small rupture accidents in ATR. LOTRAC/HEATUP

    International Nuclear Information System (INIS)

    1997-08-01

    In the evaluation of thermo-hydraulic and fuel temperature transient changes in the events which are classified in medium and small rupture accidents of reactor coolant loss that is the safety evaluation event of the ATR, the analysis code for synthetic thermo-hydraulic transient change at the time of medium and small ruptures LOTRAC and the detailed analysis code for fuel temperature HEATUP are used, respectively. By using the LOTAC, the thermo-hydraulic behavior of reactor cooling facility and the temperature behavior of fuel at the time of blow-down are analyzed, and also the characteristics of changing reactor thermal output is analyzed, considering the functioning characteristics of emergency core cooling system. Based on the data of thermo-hydraulic behavior obtained by the LOTRAC, the time of beginning the turn-around of fuel cladding tube temperature obtained by the data of ECCS pouring characteristics, the heat transfer rate after the turn-around and so on, the detailed temperature change of fuel elements is analyzed by the HEATUP, and the highest temperature and the amount of oxidation of fuel cladding tubes are determined. The LOTRAC code, the HEATUP code, various analysis models, and rupture simulation experiment are reported. (K.I.)

  7. Analysis code for pressure in reactor containment vessel of ATR. CONPOL

    International Nuclear Information System (INIS)

    1997-08-01

    For the evaluation of the pressure and temperature in containment vessels in the events which are classified in the abnormal change of pressure, atmosphere and others in reactor containment vessels in accident among the safety evaluation events of the ATR, the analysis code for the pressure in reactor containment vessels CONPOL is used. In this report, the functions of the analysis code and the analysis model are shown. By using this analysis code, the rise of the pressure and temperature in a containment vessel is evaluated when loss of coolant accident occurs, and high temperature, high pressure coolant flows into it. This code possesses the functions of computing blow-down quantity and heat dissipation from reactor cooling facility, steam condensing heat transfer to containment vessel walls, and the cooling effect by containment vessel spray system. As for the analysis techniques, the models of reactor cooling system, containment vessel and steam discharge pool, and the computation models for the pressure and temperature in containment vessels, wall surface temperature, condensing heat transfer, spray condensation and blow-down are explained. The experimental analysis of the evaluation of the pressure and temperature in containment vessels at the time of loss of coolant accident is reported. (K.I.)

  8. On three-dimensional nuclear thermo-hydraulic computation techniques for ATR

    International Nuclear Information System (INIS)

    1997-08-01

    The three-dimensional computation code for nuclear thermo-hydraulic combination core LAYMON-2A is used for the calculation of the power distribution and the control rod reactivity value of the ATR. This code possesses various functions which are required for planning the core operation such as the search function for critical boric acid concentration, and can do various simulation calculations such as core burning calculation. Further, the three-dimensional analysis code for xenon dynamic characteristics in the core LAYMON-2C, in which the dynamic characteristic equation of xenon-samarium was incorporated into the LAYMON-2A code can take the change with time lapse of xenon-samarium concentration accompanying the change of power level and power distribution into account, and it is used for the analysis of the spatial vibration characteristics of power and the regional power control characteristics due to xenon in the core. As to the LAYMON-2A, the computation flow, power distribution and thermo-hydraulic computation models, and critical search function are explained. As to the LAYMON-2C, the computation flow is described. The comparison of the calculated values by using the LAYMON-2A code and the operation data of the Fugen is reported. (K.I.)

  9. Behavior of irradiated ATR/MOX fuel under reactivity initiated accident conditions (Joint research)

    International Nuclear Information System (INIS)

    Sasajima, Hideo; Fuketa, Toyoshi; Nakamura, Takehiko; Nakamura, Jinichi; Uetsuka, Hiroshi

    2000-03-01

    Pulse irradiation experiments with irradiated ATR/MOX fuel rods of 20 MWd/kgHM were conducted at the NSRR in JAERI to study the transient behavior of MOX fuel rod under reactivity initiated accident conditions. Four pulse irradiation experiments were performed with peak fuel enthalpy ranging from 335 J/g to 586 J/g, resulted in no failure of fuel rods. Deformation of the fuel rods due to PCMI occurred in the experiments with peak fuel enthalpy above 500 J/g. Significant fission gas release up to 20% was measured by rod puncture measurement. The generation of fine radial cracks in pellet periphery, micro-cracks and boundary separation over the entire region of pellet were observed. These microstructure changes might contribute to the swelling of fuel pellets during the pulse irradiation. This could cause the large radial deformation of fuel rod and high fission gas release when the pulse irradiation conducted at relatively high peak fuel enthalpy. In addition, fine grain structures around the plutonium spot and cauliflower structure in cavity of the plutonium spot were observed in the outer region of the fuel pellet. (author)

  10. Computer analysis of ATR-FTIR spectra of paint samples for forensic purposes

    Science.gov (United States)

    Szafarska, Małgorzata; Woźniakiewicz, Michał; Pilch, Mariusz; Zięba-Palus, Janina; Kościelniak, Paweł

    2009-04-01

    A method of subtraction and normalization of IR spectra (MSN-IR) was developed and successfully applied to extract mathematically the pure paint spectrum from the spectrum of paint coat on different bases, both acquired by the Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) technique. The method consists of several stages encompassing several normalization and subtraction processes. The similarity of the spectrum obtained with the reference spectrum was estimated by means of the normalized Manhattan distance. The utility and performance of the method proposed were tested by examination of five different paints sprayed on plastic (polyester) foil and on fabric materials (cotton). It was found that the numerical algorithm applied is able - in contrast to other mathematical approaches conventionally used for the same aim - to reconstruct a pure paint IR spectrum effectively without a loss of chemical information provided. The approach allows the physical separation of a paint from a base to be avoided, hence a time and work-load of analysis to be considerably reduced. The results obtained prove that the method can be considered as a useful tool which can be applied to forensic purposes.

  11. Effects of Near Infrared Radiation on DNA. DLS and ATR-FTIR Study

    Science.gov (United States)

    Szymborska-Małek, Katarzyna; Komorowska, Małgorzata; Gąsior-Głogowska, Marlena

    2018-01-01

    We presume that the primary effect of Near Infrared (NIR) radiation on aqueous solutions of biological molecules concerns modification of hydrogen bonded structures mainly the global and the hydration shell water molecules. Since water has a significant influence on the DNA structure, we expect that the thermal stability of DNA could be modified by NIR radiation. The herring sperm DNA was exposed to NIR radiation (700-1100 nm) for 5, 10, and 20 min periods. The temperature dependent infrared measurements were done for the thin films formed on the diamond ATR crystal from evaporated DNA solutions exposed and unexposed to NIR radiation. For the NIR-treated samples (at room temperature) the B form was better conserved than in the control sample independently of the irradiation period. Above 50 °C a considerable increase in the A form was only observed for 10 min NIR exposed samples. The hydrodynamic radius, (Rh), studied by the dynamic light scattering, showed drastic decrease with the increasing irradiation time. Principal components analysis (PCA) allowed to detect the spectral features correlated with the NIR effect and thermal stability of the DNA films. Obtained results strongly support the idea that the photoionization of water by NIR radiation in presence of DNA molecules is the main factor influencing on its physicochemical properties.

  12. E2F1 transcription is induced by genotoxic stress through ATM/ATR activation.

    Science.gov (United States)

    Carcagno, Abel L; Ogara, María F; Sonzogni, Silvina V; Marazita, Mariela C; Sirkin, Pablo F; Ceruti, Julieta M; Cánepa, Eduardo T

    2009-05-01

    E2F1, a member of the E2F family of transcription factors, plays a critical role in controlling both cell cycle progression and apoptotic cell death in response to DNA damage and oncogene activation. Following genotoxic stresses, E2F1 protein is stabilized by phosphorylation and acetylation driven to its accumulation. The aim of the present work was to examine whether the increase in E2F1 protein levels observed after DNA damage is only a reflection of an increase in E2F1 protein stability or is also the consequence of enhanced transcription of the E2F1 gene. The data presented here demonstrates that UV light and other genotoxics induce the transcription of E2F1 gene in an ATM/ATR dependent manner, which results in increasing E2F1 mRNA and protein levels. After genotoxic stress, transcription of cyclin E, an E2F1 target gene, was significantly induced. This induction was the result of two well-differentiated effects, one of them dependent on de novo protein synthesis and the other on the protein stabilization. Our results strongly support a transcriptional effect of DNA damaging agents on E2F1 expression. The results presented herein uncover a new mechanism involving E2F1 in response to genotoxic stress.

  13. Influence of DMPS on the water retention capacity of electroporated stratum corneum: ATR-FTIR study.

    Science.gov (United States)

    Sckolnick, Maria; Hui, Sek-Wen; Sen, Arindam

    2008-02-28

    Anionic lipids like phosphatidylserine are known to significantly enhance electroporation mediated transepidermal transport of polar solutes of molecular weights up to 10kDa. The underlying mechanism of the effect of anionic lipids on transdermal transport is not fully understood. The main barrier to transdermal transport lies within the intercellular lipid matrix (ILM) of the stratum corneum (SC) and our previous studies indicate that dimyristoyl phosphatidylserine (DMPS) can perturb the packing of this lipid matrix. Here we report on our investigation on water retention in the SC following electroporation in the presence and the absence of DMPS. The water content in the outer most layers of the SC of full thickness porcine skin was determined using ATR-FTIR-spectroscopy. The results show that in the presence of DMPS, the SC remains in a state of enhanced hydration for longer periods after electroporation. This increase in water retention in the SC by DMPS is likely to play an important role in trans-epidermal transport, since improved hydration of the skin barrier can be expected to increase the partitioning of polar solutes and possibly the permeability.

  14. Thermal deterioration of virgin olive oil monitored by ATR-FTIR analysis of trans content.

    Science.gov (United States)

    Tena, Noelia; Aparicio, Ramón; García-González, Diego L

    2009-11-11

    The monitoring of frying oils by an effective and rapid method is one of the demands of food companies and small food retailers. In this work, a method based on ATR-FTIR has been developed for monitoring the oil degradation in frying procedures. The IR bands changing during frying in sunflower, soybean, and virgin olive oils have been examined in their linear relationship with the content of total polar compounds, which is a preferred parameter for frying control. The bands assigned to conjugated and isolated trans double bonds that are commonly used for the determination of trans content provided the best relationships. Then, the area covering 978-960 cm(-1) was chosen to build a model for predicting polar material content for the particular case of virgin olive oil. A virgin olive oil was heated up to 94 h, and samples collected every 2 h constituted the training set. These samples were analyzed to obtain their FTIR spectra and to determine the composition of fatty acids and the content of total polar compounds. The excellent results predicting the polar material content (adjusted R(2) 0.997) was successfully validated with an external set of samples. The analysis of the fatty acid composition confirmed the relationship between the trans content and the content of total polar compounds.

  15. The degradation potential of PET bottles in the marine environment: An ATR-FTIR based approach

    Science.gov (United States)

    Ioakeimidis, C.; Fotopoulou, K. N.; Karapanagioti, H. K.; Geraga, M.; Zeri, C.; Papathanassiou, E.; Galgani, F.; Papatheodorou, G.

    2016-03-01

    The dominance and persistence of plastic debris in the marine environment are well documented. No information exists in respect to their lifespan in the marine environment. Nevertheless, the degradation potential of plastic litter items remains a critical issue for marine litter research. In the present study, polyethylene terephthalate bottles (PETs) collected from the submarine environment were characterized using ATR-FTIR in respect to their degradation potential attributed to environmental conditions. A temporal indication was used as indicative to the years of presence of the PETs in the environment as debris. PETs seem to remain robust for approximately fifteen years. Afterwards, a significant decrease of the native functional groups was recorded; some even disappear; or new-not typical for PETs-are created. At a later stage, using the PET time series collected from the Saronikos Gulf (Aegean Sea-E. Mediterranean), it was possible to date bottles that were collected from the bottom of the Ionian Sea (W. Greece). It is the first time that such a study has been conducted with samples that were actually degraded in the marine environment.

  16. Rapid and Simultaneous Prediction of Eight Diesel Quality Parameters through ATR-FTIR Analysis

    Science.gov (United States)

    Hatanaka, Rafael Rodrigues; Flumignan, Danilo Luiz; de Oliveira, José Eduardo

    2018-01-01

    Quality assessment of diesel fuel is highly necessary for society, but the costs and time spent are very high while using standard methods. Therefore, this study aimed to develop an analytical method capable of simultaneously determining eight diesel quality parameters (density; flash point; total sulfur content; distillation temperatures at 10% (T10), 50% (T50), and 85% (T85) recovery; cetane index; and biodiesel content) through attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy and the multivariate regression method, partial least square (PLS). For this purpose, the quality parameters of 409 samples were determined using standard methods, and their spectra were acquired in ranges of 4000–650 cm−1. The use of the multivariate filters, generalized least squares weighting (GLSW) and orthogonal signal correction (OSC), was evaluated to improve the signal-to-noise ratio of the models. Likewise, four variable selection approaches were tested: manual exclusion, forward interval PLS (FiPLS), backward interval PLS (BiPLS), and genetic algorithm (GA). The multivariate filters and variables selection algorithms generated more fitted and accurate PLS models. According to the validation, the FTIR/PLS models presented accuracy comparable to the reference methods and, therefore, the proposed method can be applied in the diesel routine monitoring to significantly reduce costs and analysis time. PMID:29629209

  17. Rapid and Simultaneous Prediction of Eight Diesel Quality Parameters through ATR-FTIR Analysis

    Directory of Open Access Journals (Sweden)

    Maurilio Gustavo Nespeca

    2018-01-01

    Full Text Available Quality assessment of diesel fuel is highly necessary for society, but the costs and time spent are very high while using standard methods. Therefore, this study aimed to develop an analytical method capable of simultaneously determining eight diesel quality parameters (density; flash point; total sulfur content; distillation temperatures at 10% (T10, 50% (T50, and 85% (T85 recovery; cetane index; and biodiesel content through attenuated total reflection Fourier transform infrared (ATR-FTIR spectroscopy and the multivariate regression method, partial least square (PLS. For this purpose, the quality parameters of 409 samples were determined using standard methods, and their spectra were acquired in ranges of 4000–650 cm−1. The use of the multivariate filters, generalized least squares weighting (GLSW and orthogonal signal correction (OSC, was evaluated to improve the signal-to-noise ratio of the models. Likewise, four variable selection approaches were tested: manual exclusion, forward interval PLS (FiPLS, backward interval PLS (BiPLS, and genetic algorithm (GA. The multivariate filters and variables selection algorithms generated more fitted and accurate PLS models. According to the validation, the FTIR/PLS models presented accuracy comparable to the reference methods and, therefore, the proposed method can be applied in the diesel routine monitoring to significantly reduce costs and analysis time.

  18. Rapid and Simultaneous Prediction of Eight Diesel Quality Parameters through ATR-FTIR Analysis.

    Science.gov (United States)

    Nespeca, Maurilio Gustavo; Hatanaka, Rafael Rodrigues; Flumignan, Danilo Luiz; de Oliveira, José Eduardo

    2018-01-01

    Quality assessment of diesel fuel is highly necessary for society, but the costs and time spent are very high while using standard methods. Therefore, this study aimed to develop an analytical method capable of simultaneously determining eight diesel quality parameters (density; flash point; total sulfur content; distillation temperatures at 10% (T10), 50% (T50), and 85% (T85) recovery; cetane index; and biodiesel content) through attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy and the multivariate regression method, partial least square (PLS). For this purpose, the quality parameters of 409 samples were determined using standard methods, and their spectra were acquired in ranges of 4000-650 cm -1 . The use of the multivariate filters, generalized least squares weighting (GLSW) and orthogonal signal correction (OSC), was evaluated to improve the signal-to-noise ratio of the models. Likewise, four variable selection approaches were tested: manual exclusion, forward interval PLS (FiPLS), backward interval PLS (BiPLS), and genetic algorithm (GA). The multivariate filters and variables selection algorithms generated more fitted and accurate PLS models. According to the validation, the FTIR/PLS models presented accuracy comparable to the reference methods and, therefore, the proposed method can be applied in the diesel routine monitoring to significantly reduce costs and analysis time.

  19. Advanced Test Reactor National Scientific User Facility (ATR NSUF) Monthly Report October 2014

    Energy Technology Data Exchange (ETDEWEB)

    Ogden, Dan [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2014-10-01

    Advanced Test Reactor National Scientific User Facility (ATR NSUF) Monthly Report October 2014 Highlights • Rory Kennedy, Dan Ogden and Brenden Heidrich traveled to Germantown October 6-7, for a review of the Infrastructure Management mission with Shane Johnson, Mike Worley, Bradley Williams and Alison Hahn from NE-4 and Mary McCune from NE-3. Heidrich briefed the group on the project progress from July to October 2014 as well as the planned path forward for FY15. • Jim Cole gave two invited university seminars at Ohio State University and University of Florida, providing an overview of NSUF including available capabilities and the process for accessing facilities through the peer reviewed proposal process. • Jim Cole and Rory Kennedy co-chaired the NuMat meeting with Todd Allen. The meeting, sponsored by Elsevier publishing, was held in Clearwater, Florida, and is considered one of the premier nuclear fuels and materials conferences. Over 340 delegates attended with 160 oral and over 200 posters presented over 4 days. • Thirty-one pre-applications were submitted for NSUF access through the NE-4 Combined Innovative Nuclear Research Funding Opportunity Announcement. • Fourteen proposals were received for the NSUF Rapid Turnaround Experiment Summer 2014 call. Proposal evaluations are underway. • John Jackson and Rory Kennedy attended the Nuclear Fuels Industry Research meeting. Jackson presented an overview of ongoing NSUF industry research.

  20. ATR-FTIR spectroscopy and quantitative multivariate analysis of paints and coating materials

    Science.gov (United States)

    Hayes, Philippa Alice; Vahur, Signe; Leito, Ivo

    2014-12-01

    The applicability of ATR-FTIR spectroscopy with partial least squares (PLS) data analysis was evaluated for quantifying the components of mixtures of paint binding media and pigments, and alkyd resins. PLS methods were created using a number of standard mixtures. Validation and measurement uncertainty estimation was carried out. Binary, ternary and quaternary mixtures of several common binding media and pigments were quantified, with standard measurement uncertainties in most cases below 3 g/100 g. Classes of components - aromatic anhydrides and alcohols - used in alkyd resin synthesis were also successfully quantified, with standard uncertainties in the range of 2-3 g/100 g. This is a more demanding application because in alkyd resins aromatic anhydrides and alcohols have reacted to form a polyester, and are not present in their original forms. Once a PLS method has been calibrated, analysis time and cost are significantly reduced from typical quantitative methods such as GC/MS. This is beneficial in the case of routine analysis where the components are known.

  1. A Neural Network Controller New Methodology for the ATR-42 Morphing Wing Actuation

    Directory of Open Access Journals (Sweden)

    Abdallah Ben MOSBAH

    2016-06-01

    Full Text Available A morphing wing model is used to improve aircraft performance. To obtain the desired airfoils, electrical actuators are used, which are installed inside of the wing to morph its upper surface in order to obtain its desired shape. In order to achieve this objective, a robust position controller is needed. In this research, a design and test validation of a controller based on neural networks is presented. This controller was composed by a position controller and a current controller to manage the current consumed by the electrical actuators to obtain its desired displacement. The model was tested and validated using simulation and experimental tests. The results obtained with the proposed controller were compared to the results given by the PID controller. Wind tunnel tests were conducted in the Price-Païdoussis Wind Tunnel at the LARCASE laboratory in order to calculate the pressure coefficient distribution on an ATR-42 morphing wing model for different flow conditions. The pressure coefficients obtained experimentally were compared with their numerical values given by XFoil software.

  2. Quantitative analysis of anti-inflammatory drugs using FTIR-ATR spectrometry

    Science.gov (United States)

    Hassib, Sonia T.; Hassan, Ghaneya S.; El-Zaher, Asmaa A.; Fouad, Marwa A.; Taha, Enas A.

    2017-11-01

    Four simple, accurate, sensitive and economic Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopic (ATR-FTIR) methods have been developed for the quantitative estimation of some non-steroidal anti-inflammatory drugs. The first method involves the determination of Etodolac by direct measurement of the absorbance at 1716 cm- 1. In the second method, the second derivative of the IR spectra of Tolfenamic acid and its reported degradation product (2-chlorobenzoic acid) was used and the amplitudes were measured at 1084.27 cm- 1 and 1056.02 cm- 1 for Tolfenamic acid and 2-chlorobenzoic acid, respectively. The third method used the first derivative of the IR spectra of Bumadizone and its reported degradation product, N,N-diphenylhydrazine and the amplitudes were measured at 2874.98 cm- 1 and 2160.32 cm- 1 for Bumadizone and N,N-diphenylhydrazine, respectively. The fourth method depends on measuring the amplitude of Diacerein at 1059.18 cm- 1 and of rhein, its reported degradation product, at 1079.32 cm- 1 in their first derivative spectra. The four methods were successfully applied on the pharmaceutical formulations by extracting the active constituent in chloroform and the extract was directly measured in liquid phase mode using a specific cell. Moreover, validation of these methods was carried out following International Conference of Harmonisation (ICH) guidelines.

  3. The Conference Proceedings of the 2003 Air Transport Research Society (ATRS) World Conference, Volume 5

    Science.gov (United States)

    Bowen, Brent (Editor); Gudmundsson, Sveinn (Editor); Oum, Tae (Editor)

    2003-01-01

    The UNO Aviation Institute Monograph Series began in 1994 as a key component of the education outreach and information transfer missions of the Aviation Institute and the NASA Nebraska Space Grant & EPSCoR Programs. The series is an outlet for aviation materials to be indexed and disseminated through an efficient medium. Publications are welcome in all aspects of aviation. Publication formats may include, but are not limited to, conference proceedings, bibliographies, research reports, manuals, technical reports, and other documents that should be archived and indexed for future reference by the aviation and world wide communities. The Conference proceedings of the 2003 Air Transport Research Society (ATRS) world conference, volume 5 is presented. The topics include: 1) The Temporal Configuration of Airline Networks in Europe; 2) Determination and Applications of Environmental Costs at Different Sized Airports-Aircraft Noise and Engine Emissions; 3) Cost Effective Measures to Reduce CO2 Emissions in the Air Freight Sector; 4) An Assessment of the Sustainability of Air Transport System: Quantification of Indicators; 5) Regulation, Competition and Network Evolution in Aviation; 6) Regulation in the Air: Price and Frequency Cap; 7) Industry Consolidation and Future Airline Network Structures in Europe; 8) Application of Core Theory to the U.S. Airline Industry; 9) Air Freight Transshipment Route Choice Analysis; 10) A Fuzzy Approach of the Competition on Air Transport Market; and 11) Developing Passenger Demand Models for International Aviation from/to Egypt: A Case Study of Cairo Airport and Egyptair.

  4. Forensic Hair Differentiation Using Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) Spectroscopy.

    Science.gov (United States)

    Manheim, Jeremy; Doty, Kyle C; McLaughlin, Gregory; Lednev, Igor K

    2016-07-01

    Hair and fibers are common forms of trace evidence found at crime scenes. The current methodology of microscopic examination of potential hair evidence is absent of statistical measures of performance, and examiner results for identification can be subjective. Here, attenuated total reflection (ATR) Fourier transform-infrared (FT-IR) spectroscopy was used to analyze synthetic fibers and natural hairs of human, cat, and dog origin. Chemometric analysis was used to differentiate hair spectra from the three different species, and to predict unknown hairs to their proper species class, with a high degree of certainty. A species-specific partial least squares discriminant analysis (PLSDA) model was constructed to discriminate human hair from cat and dog hairs. This model was successful in distinguishing between the three classes and, more importantly, all human samples were correctly predicted as human. An external validation resulted in zero false positive and false negative assignments for the human class. From a forensic perspective, this technique would be complementary to microscopic hair examination, and in no way replace it. As such, this methodology is able to provide a statistical measure of confidence to the identification of a sample of human, cat, and dog hair, which was called for in the 2009 National Academy of Sciences report. More importantly, this approach is non-destructive, rapid, can provide reliable results, and requires no sample preparation, making it of ample importance to the field of forensic science. © The Author(s) 2016.

  5. DNA-PK, ATM and ATR collaboratively regulate p53-RPA interaction to facilitate homologous recombination DNA repair.

    Science.gov (United States)

    Serrano, M A; Li, Z; Dangeti, M; Musich, P R; Patrick, S; Roginskaya, M; Cartwright, B; Zou, Y

    2013-05-09

    Homologous recombination (HR) and nonhomologous end joining (NHEJ) are two distinct DNA double-stranded break (DSB) repair pathways. Here, we report that DNA-dependent protein kinase (DNA-PK), the core component of NHEJ, partnering with DNA-damage checkpoint kinases ataxia telangiectasia mutated (ATM) and ATM- and Rad3-related (ATR), regulates HR repair of DSBs. The regulation was accomplished through modulation of the p53 and replication protein A (RPA) interaction. We show that upon DNA damage, p53 and RPA were freed from a p53-RPA complex by simultaneous phosphorylations of RPA at the N-terminus of RPA32 subunit by DNA-PK and of p53 at Ser37 and Ser46 in a Chk1/Chk2-independent manner by ATR and ATM, respectively. Neither the phosphorylation of RPA nor of p53 alone could dissociate p53 and RPA. Furthermore, disruption of the release significantly compromised HR repair of DSBs. Our results reveal a mechanism for the crosstalk between HR repair and NHEJ through the co-regulation of p53-RPA interaction by DNA-PK, ATM and ATR.

  6. RNA-processing proteins regulate Mec1/ATR activation by promoting generation of RPA-coated ssDNA.

    Science.gov (United States)

    Manfrini, Nicola; Trovesi, Camilla; Wery, Maxime; Martina, Marina; Cesena, Daniele; Descrimes, Marc; Morillon, Antonin; d'Adda di Fagagna, Fabrizio; Longhese, Maria Pia

    2015-02-01

    Eukaryotic cells respond to DNA double-strand breaks (DSBs) by activating a checkpoint that depends on the protein kinases Tel1/ATM and Mec1/ATR. Mec1/ATR is activated by RPA-coated single-stranded DNA (ssDNA), which arises upon nucleolytic degradation (resection) of the DSB. Emerging evidences indicate that RNA-processing factors play critical, yet poorly understood, roles in genomic stability. Here, we provide evidence that the Saccharomyces cerevisiae RNA decay factors Xrn1, Rrp6 and Trf4 regulate Mec1/ATR activation by promoting generation of RPA-coated ssDNA. The lack of Xrn1 inhibits ssDNA generation at the DSB by preventing the loading of the MRX complex. By contrast, DSB resection is not affected in the absence of Rrp6 or Trf4, but their lack impairs the recruitment of RPA, and therefore of Mec1, to the DSB. Rrp6 and Trf4 inactivation affects neither Rad51/Rad52 association nor DSB repair by homologous recombination (HR), suggesting that full Mec1 activation requires higher amount of RPA-coated ssDNA than HR-mediated repair. Noteworthy, deep transcriptome analyses do not identify common misregulated gene expression that could explain the observed phenotypes. Our results provide a novel link between RNA processing and genome stability. © 2014 The Authors.

  7. Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis

    Directory of Open Access Journals (Sweden)

    Adam Oleszko

    2015-01-01

    Full Text Available During a haemodialysis (HD, because of the contact of blood with the surface of the dialyser, the immune system becomes activated and reactive oxygen species (ROS are released into plasma. Particularly exposed to the ROS are lipids and proteins contained in plasma, which undergo peroxidation. The main breakdown product of oxidized lipids is the malondialdehyde (MDA. A common method for measuring the concentration of MDA is a thiobarbituric acid reactive substances (TBARS method. Despite the formation of MDA in plasma during HD, its concentration decreases because it is removed from the blood in the dialyser. Therefore, this research proposes the Fourier Transform Infrared Attenuated Total Reflectance (FTIR-ATR spectroscopy, which enables determination of primary peroxidation products. We examined the influence of the amount of hydrogen peroxide added to lipid suspension that was earlier extracted from plasma specimen on lipid peroxidation with use of TBARS and FTIR-ATR methods. Linear correlation between these methods was shown. The proposed method was effective during the evaluation of changes in the extent of lipid peroxidation in plasma during a haemodialysis in sheep. A measurement using the FTIR-ATR showed an increase in plasma lipid peroxidation after 15 and 240 minutes of treatment, while the TBARS concentration was respectively lower.

  8. Validation of ATR FT-IR to identify polymers of plastic marine debris, including those ingested by marine organisms

    Science.gov (United States)

    Jung, Melissa R.; Horgen, F. David; Orski, Sara V.; Rodriguez, Viviana; Beers, Kathryn L.; Balazs, George H.; Jones, T. Todd; Work, Thierry M.; Brignac, Kayla C.; Royer, Sarah-Jeanne; Hyrenbach, David K.; Jensen, Brenda A.; Lynch, Jennifer M.

    2018-01-01

    Polymer identification of plastic marine debris can help identify its sources, degradation, and fate. We optimized and validated a fast, simple, and accessible technique, attenuated total reflectance Fourier transform infrared spectroscopy (ATR FT-IR), to identify polymers contained in plastic ingested by sea turtles. Spectra of consumer good items with known resin identification codes #1–6 and several #7 plastics were compared to standard and raw manufactured polymers. High temperature size exclusion chromatography measurements confirmed ATR FT-IR could differentiate these polymers. High-density (HDPE) and low-density polyethylene (LDPE) discrimination is challenging but a clear step-by-step guide is provided that identified 78% of ingested PE samples. The optimal cleaning methods consisted of wiping ingested pieces with water or cutting. Of 828 ingested plastics pieces from 50 Pacific sea turtles, 96% were identified by ATR FT-IR as HDPE, LDPE, unknown PE, polypropylene (PP), PE and PP mixtures, polystyrene, polyvinyl chloride, and nylon.

  9. Improving precursor adsorption characteristics in ATR-FTIR spectroscopy with a ZrO{sub 2} nanoparticle coating

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jaeseo [Korea Research Institute of Standards and Science, Center for Vacuum Technology (Korea, Republic of); Mun, Jihun [University of Science and Technology, Department of Advanced Device Technology (Korea, Republic of); Shin, Jae-Soo; Kim, Jongho; Park, Hee Jung [Daejeon University, Department of Advanced Materials Engineering (Korea, Republic of); Kang, Sang-Woo, E-mail: swkang@kriss.re.kr [Korea Research Institute of Standards and Science, Center for Vacuum Technology (Korea, Republic of)

    2017-02-15

    Nanoparticles were applied to a crystal surface to increase its precursor adsorption efficiency in an attenuated total reflection Fourier transform infrared (ATR-FTIR) spectrometer. Nanoparticles with varying dispersion stabilities were employed and the resulting precursor adsorption characteristics were assessed. The size of the nanoparticles was <100 nm (TEM). In order to vary the dispersion stability, ZrO{sub 2} nanoparticles were dispersed in aqueous solutions of different pH. The ZrO{sub 2} dispersion solutions were analyzed using scanning electron microscopy (SEM) while particle distribution measurements were analyzed using electrophoretic light scattering (ELS) and dynamic light scattering (DLS) techniques. ZrO{sub 2} nanoparticles dispersed in solutions of pH 3 and 11 exhibited the most stable zeta potentials (≥+30 or ≤−30 mV); these observations were confirmed by SEM analysis and particle distribution measurements. Hexamethyldisilazane (HMDS) was used as a precursor for ATR-FTIR spectroscopy. Consequently, when ZrO{sub 2} nanoparticle solutions with the best dispersion stabilities (pH 3 and 11) were applied to the adsorption crystal surface, the measurement efficiency of ATR-FTIR spectroscopy improved by ∼200 and 300%, respectively.

  10. HMGA2 Inhibits Apoptosis through Interaction with ATR-CHK1 Signaling Complex in Human Cancer Cells

    Directory of Open Access Journals (Sweden)

    Suchitra Natarajan

    2013-03-01

    Full Text Available The non-histone chromatin binding protein high mobility group AT-hook 2 (HMGA2 is expressed in stem cells and many cancer cells, including tumor initiating cells, but not translated in normal human somatic cells. The presence of HMGA2 is correlated with advanced neoplastic disease and poor prognosis for patients. We had previously demonstrated a role of HMGA2 in DNA repair pathways. In the present study, we employed different human tumor cell models with endogenous and exogenous expression of HMGA2 and show that upon DNA damage, the presence of HMGA2 caused an increased and sustained phosphorylation of the ataxia telangiectasia and Rad3-related kinase (ATR and its downstream target checkpoint kinase 1 (CHK1. The presence of activated pCHK1Ser296 coincided with prolonged G2/M block and increased tumor cell survival, which was enhanced further in the presence of HMGA2. Our study, thus, identifies a novel relationship between the ATR-CHK1 DNA damage response pathway and HMGA2, which may support the DNA repair function of HMGA2 in cancer cells. Furthermore, our data provide a rationale for the use of inhibitors to ATR or CHK1 and HMGA2 in the treatment of HMGA2-positive human cancer cells.

  11. The ATM and ATR inhibitors CGK733 and caffeine suppress cyclin D1 levels and inhibit cell proliferation

    International Nuclear Information System (INIS)

    Alao, John P; Sunnerhagen, Per

    2009-01-01

    The ataxia telangiectasia mutated (ATM) and the ATM- related (ATR) kinases play a central role in facilitating the resistance of cancer cells to genotoxic treatment regimens. The components of the ATM and ATR regulated signaling pathways thus provide attractive pharmacological targets, since their inhibition enhances cellular sensitivity to chemo- and radiotherapy. Caffeine as well as more specific inhibitors of ATM (KU55933) or ATM and ATR (CGK733) have recently been shown to induce cell death in drug-induced senescent tumor cells. Addition of these agents to cancer cells previously rendered senescent by exposure to genotoxins suppressed the ATM mediated p21 expression required for the survival of these cells. The precise molecular pharmacology of these agents however, is not well characterized. Herein, we report that caffeine, CGK733, and to a lesser extent KU55933, inhibit the proliferation of otherwise untreated human cancer and non-transformed mouse fibroblast cell lines. Exposure of human cancer cell lines to caffeine and CGK733 was associated with a rapid decline in cyclin D1 protein levels and a reduction in the levels of both phosphorylated and total retinoblastoma protein (RB). Our studies suggest that observations based on the effects of these compounds on cell proliferation and survival must be interpreted with caution. The differential effects of caffeine/CGK733 and KU55933 on cyclin D1 protein levels suggest that these agents will exhibit dissimilar molecular pharmacological profiles

  12. Radioresistance of chordoma cells is associated with the ATM/ATR pathway, in which RAD51 serves as an important downstream effector.

    Science.gov (United States)

    Zhang, Chao; Wang, Bing; Li, Lei; Li, Yawei; Li, Pengzhi; Lv, Guohua

    2017-09-01

    Surgery followed by radiotherapy is the standard treatment for chordomas, which are a rare but low-grade type of bone cancer arising from remnants of the embryonic notochord. However, disease recurrence following radiotherapy is common, most likely due to endogenous DNA repair mechanisms that promote cell survival upon radiation strikes. The ataxia telangiectasia mutated/ataxia telangiectasia mutated and Rad3 related (ATM/ATR)-mediated pathway has a critical role in DNA repair mechanisms; however, it has rarely been investigated in chordomas. In the present study, the expression of signal molecules related to the ATM/ATR pathway in chordoma tissues and adjacent normal tissues were initially examined using immunohistochemistry and western blot analysis. Chordoma U-CH1 and U-CH2 cells were subsequently used to investigate cell responses to ionizing radiation and the potential protective actions mediated by the ATM/ATR pathway. Phosphorylated (p)-ATM, p-ATR, γ-H2A histone family, member X (H2AX) and RAD51 were significantly upregulated in chordoma tissues relative to adjacent normal tissues (PATM, γ-H2AX and RAD51 expression in U-CH1 cells (PATM, p-ATR and RAD51 levels in U-CH2 cells (PATM/ATR pathway, in which RAD51 serves as an important downstream effector. Thus, RAD51 presents a promising therapeutic target for improving the outcome of radiotherapy treatment in chordomas.

  13. Dual inhibition of ATR and ATM potentiates the activity of trabectedin and lurbinectedin by perturbing the DNA damage response and homologous recombination repair.

    Science.gov (United States)

    Lima, Michelle; Bouzid, Hana; Soares, Daniele G; Selle, Frédéric; Morel, Claire; Galmarini, Carlos M; Henriques, João A P; Larsen, Annette K; Escargueil, Alexandre E

    2016-05-03

    Trabectedin (Yondelis®, ecteinascidin-743, ET-743) is a marine-derived natural product approved for treatment of advanced soft tissue sarcoma and relapsed platinum-sensitive ovarian cancer. Lurbinectedin is a novel anticancer agent structurally related to trabectedin. Both ecteinascidins generate DNA double-strand breaks that are processed through homologous recombination repair (HRR), thereby rendering HRR-deficient cells particularly sensitive. We here characterize the DNA damage response (DDR) to trabectedin and lurbinectedin in HeLa cells. Our results show that both compounds activate the ATM/Chk2 (ataxia-telangiectasia mutated/checkpoint kinase 2) and ATR/Chk1 (ATM and RAD3-related/checkpoint kinase 1) pathways. Interestingly, pharmacological inhibition of Chk1/2, ATR or ATM is not accompanied by any significant improvement of the cytotoxic activity of the ecteinascidins while dual inhibition of ATM and ATR strongly potentiates it. Accordingly, concomitant inhibition of both ATR and ATM is an absolute requirement to efficiently block the formation of γ-H2AX, MDC1, BRCA1 and Rad51 foci following exposure to the ecteinascidins. These results are not restricted to HeLa cells, but are shared by cisplatin-sensitive and -resistant ovarian carcinoma cells. Together, our data identify ATR and ATM as central coordinators of the DDR to ecteinascidins and provide a mechanistic rationale for combining these compounds with ATR and ATM inhibitors.

  14. Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) for Rapid Determination of Microbial Cell Lipid Content: Correlation with Gas Chromatography-Mass Spectrometry (GC-MS).

    Science.gov (United States)

    Millan-Oropeza, Aaron; Rebois, Rolando; David, Michelle; Moussa, Fathi; Dazzi, Alexandre; Bleton, Jean; Virolle, Marie-Joelle; Deniset-Besseau, Ariane

    2017-10-01

    There is a growing interest worldwide for the production of renewable oil without mobilizing agriculture lands; fast and reliable methods are needed to identify highly oleaginous microorganisms of potential industrial interest. The aim of this study was to demonstrate the relevance of attenuated total reflection (ATR) spectroscopy to achieve this goal. To do so, the total lipid content of lyophilized samples of five Streptomyces strains with varying lipid content was assessed with two classical quantitative but time-consuming methods, gas chromatography-mass spectrometry (GC-MS) and ATR Fourier transform infrared (ATR FT-IR) spectroscopy in transmission mode with KBr pellets and the fast ATR method, often questioned for its lack of reliability. A linear correlation between these three methods was demonstrated allowing the establishment of equations to convert ATR values expressed as CO/amide I ratio, into micrograms of lipid per milligram of biomass. The ATR method proved to be as reliable and quantitative as the classical GC-MS and FT-IR in transmission mode methods but faster and more reproducible than the latter since it involves far less manipulation for sample preparation than the two others. Attenuated total reflection could be regarded as an efficient fast screening method to identify natural or genetically modified oleaginous microorganisms by the scientific community working in the field of bio-lipids.

  15. Hubungan Kadar Ft4 Dengan Kejadian Tirotoksikosis berdasarkan Penilaian Indeks New Castle Padawanita Dewasa di Daerah Ekses Yodium

    Directory of Open Access Journals (Sweden)

    Harsa Rusda

    2013-05-01

    Full Text Available AbstrakTirotoksikosis merupakan manifestasi klinis yang terjadi akibat peningkatan kadar hormon tiroid dalam darah. Kelebihan yodium merupakan salah satu penyebab terjadinya tirotoksikosis. Ini ditandai dengan hasil pemeriksaan kadar Ekskresi Yodium Urin (EYU > 199 μg/L. Tujuan: Penelitian ini bertujuan untuk mengetahui hubungan kadar FT4 dengan kejadian tirotoksikosis berdasarkan penilaian indeks New Castle pada wanita dewasa di daerah ekses yodium. Metode: Penelitian dilakukan dengan menganalisis data yang dikumpulkan secara Cross Sectional Study terhadap 37 wanita dewasa menggunakan metoda total sampling di Nagari Koto Salak, Kecamatan Koto Salak Kabupaten Dharmasraya yang merupakan daerah ekses yodium (median EYU 323,5 μg/L. Pengumpulan data dilakukan dengan wawancara menggunakan penilaian Indeks New Castle dan pengambilan darah untuk dilakukan pemeriksaan kadar FT4 dalam serum. Hasil: Analisis univariat didapatkan jumlah penduduk wanita dewasa dengan kadar FT4 meningkat sebanyak 14 persen dan nilai rata-rata 1,71 ng/dl. Penilaian indeks New Castle dalam kategori doubtful 16 persen dan tidak ditemukan penduduk yang termasuk dalam kategori toxic. Berdasarkan hasil uji statistik Chi-square, didapatkan p value=1. Kesimpulan: Tidak terdapat hubungan yang bermakna antara kadar FT4 dalam serum dengan kejadian tirotoksikosis pada wanita dewasa di derah ekses yodium. Saran: Perlu dilakukan penyuluhan mengenai asupan yodium kepada masyarakat dan diharapkan penelitian ini dapat menjadi masukan kepada pemerintah dalam mengambil kebijakan terhadap pemberian kapsul yodium serta melakukan kontrol kadar EYU secara teratur dan berkala.Kata kunci: FT4, tirotoksikosis, indeks New Castle, wanita, ekses yodium, tiroidAbstractThyrotoxicosis is a clinical manifestation that occurs due to elevated levels of thyroid hormones in the blood. Iodine excess is one of the causes of thyrotoxicosis. This is indicated by the results of urine iodine excretion levels (EYU

  16. PERFORMA FOTOSINTESIS Kappaphycus sp. (strain Sumba YANG DIUKUR BERDASARKAN EVOLUSI OKSIGEN TERLARUT PADA BEBERAPA TINGKAT SUHU DAN CAHAYA

    Directory of Open Access Journals (Sweden)

    Lideman Lideman

    2015-03-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui pengaruh suhu dan cahaya terhadap laju fotosintesis Kappaphycus sp. (strain Sumba yang diukur berdasarkan perubahan oksigen terlarut. Pengukuran laju fotosintesis Kappaphycus sp. pertama-tama dilakukan pada suhu 20oC, 24oC, 28oC, dan 32oC pada tingkat cahaya 353 μmol photons m-2 s-1 untuk mendapatkan kurva fotosintesis versus suhu (kurva P-T. Selanjutnya, pengukuran laju fotosintesis dilakukan pada suhu 20oC, 24oC, dan 28oC dengan intensitas cahaya 9, 22, 46, 58, 87, 137, 245, 353, 487, 608, dan 789 μmol photons m-2 s-1 dan juga pengukuran laju respirasi pada tingkat cahaya 0 μmol photons m-2 s-1 untuk menghasilkan kurva fotosintesis versus cahaya (kurva P-I. Beberapa parameter fotosintesis yaitu: laju fotosintesis maksimum (Pmax, koefisien fotosintesis (α, intensitas cahaya jenuh (Ek, dan intensitas cahaya kompensasi (Ec dihitung dengan cara memplotkan kurva P-I terhadap model persamaan regresi non linear P = {Pmax x tanh (α / Pmax x I} + Rd. Hasil yang diperoleh menunjukkan bahwa laju fotosintesis tertinggi sebesar 6,92 μg O2 gww-1 min-1 dicapai pada suhu 28oC dengan tingkat cahaya 353 μmol photons m-2 s-1. Pada suhu 20oC, 24oC, dan 28oC, laju fotosintesis mencapai tingkat maksimum (Pmax pada intensitas cahaya (Ek 86,1; 154,2; dan 162,4 μmol photons m-2 s-1. Suhu yang optimum untuk aktivitas fotosintesis berkorelasi erat dengan suhu pada lingkungan budidaya di alam.

  17. PENENTUAN LOKASI BUDI DAYA RUMPUT LAUT (Euchema spp. BERDASARKAN PARAMETER LINGKUNGAN DI PERAIRAN KECAMATAN MORO, PROVINSI KEPULAUAN RIAU

    Directory of Open Access Journals (Sweden)

    I Nyoman Radiarta

    2016-11-01

    Full Text Available Penelitian ini bertujuan untuk melakukan identifikasi kelayakan perairan untuk budi daya rumput laut dengan menggunakan metode long line berdasarkan parameter lingkungan di perairan Kecamatan Moro Provinsi Kepulauan Riau. Sebanyak 87 stasiun telah dikumpulkan selama survai lapangan pada bulan Agustus dan Oktober 2004. Data parameter lingkungan dan data penginderaan jauh selanjutnya dianalisis dengan menggunakan sistem informasi geografis dan multi kriteria analisis. Dari total potensial lokasi penelitian seluas 417 km2 (jarak 1 km dari garis pantai, kategori sangat layak di temukan di perairan sekitar Pulau Sugie dan Pulau Combol seluas 110 km2. Hasil verifikasi dari klasifikasi tingkat kelayakan menunjukkan bahwa sekitar 44% dari budi daya yang ada menempati kategori sangat layak. Terdapat sekitar 6% yang menempati perairan dengan kategori tidak layak. This study was conducted to identify suitability site for seaweed culture using long line method based on environmental parameters in adjacent water of Moro Sub District, Riau Island Province. Field observation was primary data sources used in this study that was conducted on August and October 2000. Total of 87 sampling stations were collected during the field survey. Environmental data together with remote sensing data were analyzed using geographic information system and multi criteria analysis. The final result showed that from the total potential site of about 417 km2 (1 km buffer from coastline, area around Sugie Island and Combol Island (about 110 km2 were classified as highly suitable. The result has been verified with the existing seaweed aquaculture. About 44% of existing seaweed culture matched with highly suitable site and about 6% was located in unsuitable site.

  18. Simultaneous determination of three surfactants and water in shampoo and liquid soap by ATR-FTIR.

    Science.gov (United States)

    Carolei, Luciano; Gutz, Ivano G R

    2005-03-31

    It is demonstrated for the first time that the principal constituents of a shampoo as well as of a liquid soap -three surfactants and water- can be determined directly, simultaneously and quickly in undiluted samples by attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy in the middle infrared region, despite the broad absorption bands of the solvent. Two of the surfactants, sodium lauryl ether sulfate (SLES) and cocoamidopropyl betaine (CAPB), are common to both formulations; alkylpolyglucoside (APG) is the third surfactant of the liquid soap and cocodiethanolamide (CDEA), the corresponding ingredient of the shampoo. Absorbance data of the undiluted samples and of the calibration standards was collected in the middle infrared region of the spectrum (800-1600 and 1900-3000cm(-1)). Two methods of multivariate quantification were compared: classical least squares (CLS), where absorbance data measured at 200 wavenumbers was processed, and inverse least squares (ILS), where data at 10 selected wavenumbers was analyzed. A spectra normalization procedure, based on a dominating water band, was examined. Twenty-seven standard mixtures were used for each application, consisting of all combinations at three concentration levels of each surfactant, respectively the lower limit, the expected value and the upper limit accepted in quality control. By favoring wavenumbers where absorption bands of the minor components (APG in the liquid soap and CDEA in the shampoo) are more intense, good results were obtained for 18 simulated samples of shampoo and 18 samples of liquid soap, no matter if calculations were made by CLS or ILS. The relative errors for water (major component, 84-88%) and SLES (7-10%) were always below 2%; for CAPB (2-4%), APG (<2%) and CDEA (<2%), they occasionally reached 5% of the component, an uncertainty of less than 0.07% in terms of the sample weight.

  19. [Research on Rapid Discrimination of Edible Oil by ATR Infrared Spectroscopy].

    Science.gov (United States)

    Ma, Xiao; Yuan, Hong-fu; Song, Chun-feng; Hu, Ai-qin; Li, Xiao-yu; Zhao, Zhong; Li, Xiu-qin; Guo Zhen; Zhu, Zhi-qiang

    2015-07-01

    A rapid discrimination method of edible oils, KL-BP model, was proposed by attenuated total reflectance infrared spectroscopy. The model extracts the characteristic of classification from source data by KL and reduces data dimension at the same time. Then the neural network model is constructed by the new data which as the input of the model. 84 edible oil samples which include sesame oil, corn oil, canola oil, blend oil, sunflower oil, peanut oil, olive oil, soybean oil and tea seed oil, were collected and their infrared spectra determined using an ATR FT-IR spectrometer. In order to compare the method performance, principal component analysis (PCA) direct-classification model, KL direct-classification model, PLS-DA model, PCA-BP model and KL-BP model are constructed in this paper. The results show that the recognition rates of PCA, PCA-BP, KL, PLS-DA and KL-BP are 59.1%, 68.2%, 77.3%, 77.3% and 90.9% for discriminating the 9 kinds of edible oils, respectively. KL extracts the eigenvector which make the distance between different class and distance of every class ratio is the largest. So the method can get much more classify information than PCA. BP neural network can effectively enhance the classification ability and accuracy. Taking full of the advantages of KL in extracting more category information in dimension reducing and the features of BP neural network in self-learning, adaptive, nonlinear, the KL-BP method has the best classification ability and recognition accuracy and great importance for rapidly recognizing edible oil in practice.

  20. Evaluation of [18F]-ATRi as PET tracer for in vivo imaging of ATR in mouse models of brain cancer

    International Nuclear Information System (INIS)

    Carlucci, Giuseppe; Carney, Brandon; Sadique, Ahmad; Vansteene, Axel; Tang, Jun; Reiner, Thomas

    2017-01-01

    Rationale: Ataxia telangiectasia and Rad3-related (ATR) threonine serine kinase is one of the key elements in orchestrating the DNA damage response (DDR). As such, inhibition of ATR can amplify the effects of chemo- and radiation-therapy, and several ATR inhibitors (ATRi) have already undergone clinical testing in cancer. For more accurate patient selection, monitoring and staging, real-time in vivo imaging of ATR could be invaluable; the development of appropriate imaging agents has remained a major challenge. Methods: 3-amino-N-(4-[ 18 F]phenyl)-6-(4-(methylsulfonyl)phenyl) pyrazine-2-carboxamide ([ 18 F]-ATRi), a close analogue of Ve-821, (a clinical ATRi candidate), was readily accomplished similarly to already established synthetic procedures. Structurally, 18 F was introduced at the 4-position of the aromatic ring of Ve-821 for generating a labeled ATR inhibitor. In vitro experiments were conducted in U251 MG glioblastoma cell lines and ex vivo biodistribution were performed in subcutaneous U251 MG xenograft bearing athymic nude mice following microPET imaging. Results: [ 18 F]-ATRi has a similar pharmacokinetic profile to that of Ve-821. Using an U251 MG glioblastoma mouse model, we evaluated the in vivo binding efficiency of [ 18 F]-ATRi. Blood and tumor showed a statistically significant difference between mice injected with only the probe or following blocking experiment with Ve-821 (1.48 ± 0.40%ID/g vs. 0.46 ± 0.12%ID/g in tumor and 1.85 ± 0.47%ID/g vs. 0.84 ± 0.3%ID/g in blood respectively). Conclusions: [ 18 F]-ATRi represents the first 18 F positron emission tomography (PET) ATR imaging agent, and is designed on a low nanomolar and clinically relevant ATR inhibitor.

  1. Measured thermal and fast neutron fluence rates, ATR Cycle 102-A, 11/28/93 thru 1/16/94

    International Nuclear Information System (INIS)

    Murray, R.K.; Rogers, J.W.

    1994-02-01

    This report contains the thermal (2,200 m/s) and fast (E > 1MeV) neutron fluence rate data for ATR Cycle 102-A which were measured by the Radiation Measurements Laboratory (RML) as requested by the Power Reactor Programs (ATR Experiments) Radiation Measurements Work Order. This report contains fluence rate values corresponding to the particular elevations (relative to the 80 ft. core elevation) where the measurements were taken. The data in this report consists of (1) a table of the ATR power history and distribution, (2) a hard copy listing of all thermal and fast neutron fluence rates, (3) plots of both the thermal and fast neutron fluence rates, and (4) a magnetic record (3.5 inch diskette) containing a listing of only the fast neutron fluence rates, their assigned elevations and proper header identification of all monitor positions contained herein. The fluence rates reported are for the average power levels given in the table of power history and distribution. All ''H'' holder monitoring wires for this cycle are 54 inches long. All ''SR'' holder monitor wires for this cycle are 55 inches long. This length allows measurement of the full core region and makes the first count elevation 24.73 inches above core midplane. Due to the safety rod problems in the west lobe, ''BR'' holders were used in the W-1, 2, 3, and 4 positions. All ''BR'' holder monitor wires for this cycle are 56.25 inches long. The distance from the end of the wires to the first count position was 4.25 inches for all wires counted from this cycle

  2. Measured thermal and fast neutron fluence rates ATR Cycle 99-A, November 23, 1992--January 23, 1993

    International Nuclear Information System (INIS)

    Murray, R.K.; Rogers, J.W.

    1993-03-01

    This report contains the thermal (2200 m/s) and fast (E>me) neutron fluence rate data for ATR Cycle 99-A which were measured by the Radiation Measurements Laboratory (RML) as requested by the Power ReactorPrograms (ATR Experiments) Radiation Measurements Work Order. This report contains fluence rate values corresponding to the particular elevations (relative to the 80 ft. core elevation) where the measurements were taken. The data in this report consists of (1) a table of the ATR power history and distribution, (2) a hard copy listing of all thermal and fast neutron fluence rates, (3) plots of both the thermal and fast neutron fluence rates, and (4) a magnetic record (3.5 inch diskette) containing a listing of only the fast neutron fluence rates, their assigned elevations and proper header identification of all monitor positions contained herein. The fluence rates reported are for the average power levels given in the table of power history and distribution. All ''H'' holder monitor wires for this cycle are 54 inches long. All ''SR'' holder monitor wires for this cycle are 55 inches long. This length allows measurement of the full core region and makes the first count elevation 24.73 inches above core midplane. Due to the safety rod problems in the west lobe, ''BR'' holders were used in the W-1, 2, 3, and 4 positions. All ''BR'' holder monitor wires for this cycle are 56.25 inches long. The distance from the end of the wires to the first count position was 4.25 inches for all wires counted from this cycle

  3. Modeling Microalgal Biosediment Formation Based on Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) Monitoring.

    Science.gov (United States)

    Ogburn, Zachary L; Vogt, Frank

    2018-03-01

    With increasing amounts of anthropogenic pollutants being released into ecosystems, it becomes ever more important to understand their fate and interactions with living organisms. Microalgae play an important ecological role as they are ubiquitous in marine environments and sequester inorganic pollutants which they transform into organic biomass. Of particular interest in this study is their role as a sink for atmospheric CO 2 , a greenhouse gas, and nitrate, one cause of harmful algal blooms. Novel chemometric hard-modeling methodologies have been developed for interpreting phytoplankton's chemical and physiological adaptations to changes in their growing environment. These methodologies will facilitate investigations of environmental impacts of anthropogenic pollutants on chemical and physiological properties of marine microalgae (here: Nannochloropsis oculata). It has been demonstrated that attenuated total reflection Fourier transform infrared (ATR FT-IR) spectroscopy can gain insights into both and this study only focuses on the latter. From time-series of spectra, the rate of microalgal biomass settling on top of a horizontal ATR element is derived which reflects several of phytoplankton's physiological parameters such as growth rate, cell concentrations, cell size, and buoyancy. In order to assess environmental impacts on such parameters, microalgae cultures were grown under 25 different chemical scenarios covering 200-600 ppm atmospheric CO 2 and 0.35-0.75 mM dissolved NO 3 - . After recording time-series of ATR FT-IR spectra, a multivariate curve resolution-alternating least squares (MCR-ALS) algorithm extracted spectroscopic and time profiles from each data set. From the time profiles, it was found that in the considered concentration ranges only NO 3 - has an impact on the cells' physiological properties. In particular, the cultures' growth rate has been influenced by the ambient chemical conditions. Thus, the presented spectroscopic

  4. The application of ATR-FTIR spectroscopy and multivariate data analysis to study drug crystallisation in the stratum corneum.

    Science.gov (United States)

    Goh, Choon Fu; Craig, Duncan Q M; Hadgraft, Jonathan; Lane, Majella E

    2017-02-01

    Drug permeation through the intercellular lipids, which pack around and between corneocytes, may be enhanced by increasing the thermodynamic activity of the active in a formulation. However, this may also result in unwanted drug crystallisation on and in the skin. In this work, we explore the combination of ATR-FTIR spectroscopy and multivariate data analysis to study drug crystallisation in the skin. Ex vivo permeation studies of saturated solutions of diclofenac sodium (DF Na) in two vehicles, propylene glycol (PG) and dimethyl sulphoxide (DMSO), were carried out in porcine ear skin. Tape stripping and ATR-FTIR spectroscopy were conducted simultaneously to collect spectral data as a function of skin depth. Multivariate data analysis was applied to visualise and categorise the spectral data in the region of interest (1700-1500cm -1 ) containing the carboxylate (COO - ) asymmetric stretching vibrations of DF Na. Spectral data showed the redshifts of the COO - asymmetric stretching vibrations for DF Na in the solution compared with solid drug. Similar shifts were evident following application of saturated solutions of DF Na to porcine skin samples. Multivariate data analysis categorised the spectral data based on the spectral differences and drug crystallisation was found to be confined to the upper layers of the skin. This proof-of-concept study highlights the utility of ATR-FTIR spectroscopy in combination with multivariate data analysis as a simple and rapid approach in the investigation of drug deposition in the skin. The approach described here will be extended to the study of other actives for topical application to the skin. Copyright © 2016 Elsevier B.V. All rights reserved.

  5. Dynamic dependence on ATR and ATM for double-strand break repair in human embryonic stem cells and neural descendants.

    Science.gov (United States)

    Adams, Bret R; Golding, Sarah E; Rao, Raj R; Valerie, Kristoffer

    2010-04-02

    The DNA double-strand break (DSB) is the most toxic form of DNA damage. Studies aimed at characterizing DNA repair during development suggest that homologous recombination repair (HRR) is more critical in pluripotent cells compared to differentiated somatic cells in which nonhomologous end joining (NHEJ) is dominant. We have characterized the DNA damage response (DDR) and quality of DNA double-strand break (DSB) repair in human embryonic stem cells (hESCs), and in vitro-derived neural cells. Resolution of ionizing radiation-induced foci (IRIF) was used as a surrogate for DSB repair. The resolution of gamma-H2AX foci occurred at a slower rate in hESCs compared to neural progenitors (NPs) and astrocytes perhaps reflective of more complex DSB repair in hESCs. In addition, the resolution of RAD51 foci, indicative of active homologous recombination repair (HRR), showed that hESCs as well as NPs have high capacity for HRR, whereas astrocytes do not. Importantly, the ATM kinase was shown to be critical for foci formation in astrocytes, but not in hESCs, suggesting that the DDR is different in these cells. Blocking the ATM kinase in astrocytes not only prevented the formation but also completely disassembled preformed repair foci. The ability of hESCs to form IRIF was abrogated with caffeine and siRNAs targeted against ATR, implicating that hESCs rely on ATR, rather than ATM for regulating DSB repair. This relationship dynamically changed as cells differentiated. Interestingly, while the inhibition of the DNA-PKcs kinase (and presumably non-homologous endjoining [NHEJ]) in astrocytes slowed IRIF resolution it did not in hESCs, suggesting that repair in hESCs does not utilize DNA-PKcs. Altogether, our results show that hESCs have efficient DSB repair that is largely ATR-dependent HRR, whereas astrocytes critically depend on ATM for NHEJ, which, in part, is DNA-PKcs-independent.

  6. UV-induced nuclear import of XPA is mediated by importin-α4 in an ATR-dependent manner.

    Directory of Open Access Journals (Sweden)

    Zhengke Li

    Full Text Available Xeroderma pigmentosum Group A (XPA is a crucial factor in mammalian nucleotide excision repair (NER and nuclear import of XPA from the cytoplasm for NER is regulated in cellular DNA damage responses in S-phase. In this study, experiments were carried out to determine the transport mechanisms that are responsible for the UV (ultraviolet-induced nuclear import of XPA. We found that, in addition to the nuclear localization signal (NLS of XPA, importin-α4 or/and importin-α7 are required for the XPA nuclear import. Further investigation indicated that, importin-α4 and importin-α7 directly interacted with XPA in cells. Interestingly, the binding of importin-α4 to XPA was dependent on UV-irradiation, while the binding of importin-α7 was not, suggesting a role for importin-α7 in nuclear translocation of XPA in the absence of DNA damage, perhaps with specificity to certain non-S-phases of the cell-cycle. Consistent with the previous report of a dependence of UV-induced XPA nuclear import on ataxia telangiectasia and Rad3-related protein (ATR in S-phase, knockdown of ATR reduced the amount of XPA interacting with importin-α4. In contrast, the GTPase XPA binding protein 1 (XAB1, previously proposed to be required for XPA nuclear import, showed no effect on the nuclear import of XPA in our siRNA knockdown analysis. In conclusion, our results suggest that upon DNA damage transport adaptor importin-α4 imports XPA into the nucleus in an ATR-dependent manner, while XAB1 has no role in this process. In addition, these findings reveal a potential new therapeutic target for the sensitization of cancer cells to chemotherapy.

  7. Mutation analysis and characterization of ATR sequence variants in breast cancer cases from high-risk French Canadian breast/ovarian cancer families

    Directory of Open Access Journals (Sweden)

    Pichette Roxane

    2006-09-01

    Full Text Available Abstract Background Ataxia telangiectasia-mutated and Rad3-related (ATR is a member of the PIK-related family which plays, along with ATM, a central role in cell-cycle regulation. ATR has been shown to phosphorylate several tumor suppressors like BRCA1, CHEK1 and TP53. ATR appears as a good candidate breast cancer susceptibility gene and the current study was designed to screen for ATR germline mutations potentially involved in breast cancer predisposition. Methods ATR direct sequencing was performed using a fluorescent method while widely available programs were used for linkage disequilibrium (LD, haplotype analyses, and tagging SNP (tSNP identification. Expression analyses were carried out using real-time PCR. Results The complete sequence of all exons and flanking intronic sequences were analyzed in DNA samples from 54 individuals affected with breast cancer from non-BRCA1/2 high-risk French Canadian breast/ovarian families. Although no germline mutation has been identified in the coding region, we identified 41 sequence variants, including 16 coding variants, 3 of which are not reported in public databases. SNP haplotypes were established and tSNPs were identified in 73 healthy unrelated French Canadians, providing a valuable tool for further association studies involving the ATR gene, using large cohorts. Our analyses led to the identification of two novel alternative splice transcripts. In contrast to the transcript generated by an alternative splicing site in the intron 41, the one resulting from a deletion of 121 nucleotides in exon 33 is widely expressed, at significant but relatively low levels, in both normal and tumoral cells including normal breast and ovarian tissue. Conclusion Although no deleterious mutations were identified in the ATR gene, the current study provides an haplotype analysis of the ATR gene polymorphisms, which allowed the identification of a set of SNPs that could be used as tSNPs for large-scale association

  8. The Mismatch-Binding Factor MutSβ Can Mediate ATR Activation in Response to DNA Double-Strand Breaks

    Czech Academy of Sciences Publication Activity Database

    Burdová, Kamila; Mihaljevic, B.; Sturzenegger, A.; Chappidi, N.; Janščák, Pavel

    2015-01-01

    Roč. 59, č. 4 (2015), s. 603-614 ISSN 1097-2765 R&D Projects: GA ČR GAP305/10/0281; GA ČR(CZ) GA14-05743S Grant - others:Oncosuisse(CH) KLS-02344-02-2009; Swiss National Science Foundation(CH) 31003A_146206; Novartis Foundation for Medical and Biological Research(CH) 11A16 Institutional support: RVO:68378050 Keywords : Ataxia telangiectasia-mutated and Rad3-related (ATR) protein kinase * DNA-damage response * DNA Double-Strand Breaks Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 13.958, year: 2015

  9. Direct Binding to Replication Protein A (RPA)-coated Single-stranded DNA Allows Recruitment of the ATR Activator TopBP1 to Sites of DNA Damage*

    Science.gov (United States)

    Acevedo, Julyana; Yan, Shan; Michael, W. Matthew

    2016-01-01

    A critical event for the ability of cells to tolerate DNA damage and replication stress is activation of the ATR kinase. ATR activation is dependent on the BRCT (BRCA1 C terminus) repeat-containing protein TopBP1. Previous work has shown that recruitment of TopBP1 to sites of DNA damage and stalled replication forks is necessary for downstream events in ATR activation; however, the mechanism for this recruitment was not known. Here, we use protein binding assays and functional studies in Xenopus egg extracts to show that TopBP1 makes a direct interaction, via its BRCT2 domain, with RPA-coated single-stranded DNA. We identify a point mutant that abrogates this interaction and show that this mutant fails to accumulate at sites of DNA damage and that the mutant cannot activate ATR. These data thus supply a mechanism for how the critical ATR activator, TopBP1, senses DNA damage and stalled replication forks to initiate assembly of checkpoint signaling complexes. PMID:27129245

  10. ON-LINE MONITORING OF I&C TRANSMITTERS AND SENSORS FOR CALIBRATION VERIFICATION AND RESPONSE TIME TESTING WAS SUCCESSFULLY IMPLEMENTED AT ATR

    Energy Technology Data Exchange (ETDEWEB)

    Erickson, Phillip A.; O' Hagan, Ryan; Shumaker, Brent; Hashemian, H. M.

    2017-03-01

    The Advanced Test Reactor (ATR) has always had a comprehensive procedure to verify the performance of its critical transmitters and sensors, including RTDs, and pressure, level, and flow transmitters. These transmitters and sensors have been periodically tested for response time and calibration verification to ensure accuracy. With implementation of online monitoring techniques at ATR, the calibration verification and response time testing of these transmitters and sensors are verified remotely, automatically, hands off, include more portions of the system, and can be performed at almost any time during process operations. The work was done under a DOE funded SBIR project carried out by AMS. As a result, ATR is now able to save the manpower that has been spent over the years on manual calibration verification and response time testing of its temperature and pressure sensors and refocus those resources towards more equipment reliability needs. More importantly, implementation of OLM will help enhance the overall availability, safety, and efficiency. Together with equipment reliability programs of ATR, the integration of OLM will also help with I&C aging management goals of the Department of Energy and long-time operation of ATR.

  11. KLASIFIKASI INTI SEL PAP SMEAR BERDASARKAN ANALISIS TEKSTUR MENGGUNAKAN CORRELATION-BASED FEATURE SELECTION BERBASIS ALGORITMA C4.5

    Directory of Open Access Journals (Sweden)

    Toni Arifin

    2014-09-01

    Full Text Available Abstract - Pap Smear is an early examination to diagnose whether there’s indication cervical cancer or not, the process of observations were done by observing pap smear cell under the microscope. There’s so many research has been done to differentiate between normal and abnormal cell. In this research presents a classification of pap smear cell based on texture analysis. This research is using the Harlev image which amounts to 280 images, 140 images are used as training data and 140 images other are used as testing. On the texture analysis used Gray level Co-occurance Matrix (GLCM method with 5 parameters that is correlation, energy, homogeneity and entropy added by counting the value of brightness. For choose which the best attribute used correlation-based feature selection method and than used C45 algorithm for produce classification rule. The result accuracy of the classification normal and abnormal used decision tree C45 is 96,43% and errors in predicting is 3,57%. Keywords : Classification, Pap Smear cell image, texture analysis, Correlation-based feature selection, C45 algorithm. Abstrak - Pap Smear merupakan pemeriksaan dini untuk mendiagnosa apakah ada indikasi kanker serviks atau tidak, proses pengamatan dilakukan dengan mengamati sel pap smear dibawah mikroskop. Banyak penelitian yang telah dilakukan untuk membedakan antara sel normal dan abnormal. Dalam penelitian ini menyajikan klasifikasi inti sel pap smear berdasarkan analisis tektur. Citra yang digunakan dalam penelitian ini adalah citra Harlev yang berjumlah 280 citra, 140 citra digunakan sebagai data training dan 140 citra lain digunakan sebagai testing. Pada analisis tekstur mengunakan metode Gray level Co-occurrence Matrix (GLCM menggunakan 5 parameter yaitu korelasi, energi, homogenitas dan entropi ditambah dengan menghitung nilai brightness. Untuk memilih mana atribut terbaik digunakan metode correlation-based feature selection lalu digunakan algoritma C45 untuk

  12. Analisis Isi Daya Tarik Pesan Iklan Berdasarkan Elemen Print Ad pada Iklan Sabun Mandi di Majalah Femina

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    Nadya Istighfarina

    2017-07-01

    Full Text Available One of mass media in Indonesia that used for advertising is Femina magazine. The target audience of Femina is women and because of its target audience, so Femina being potential magazine for cosmetics and toiletries product to advertise. One of toiletries product is soap. In copywriting, print ad has an element that consist of headline, subheadline, body copy and ilustration. An advertisement has an appealing point that consist of informational, emotional and combination to attract the readers. The aim of this research is to identify domination of appealing point by print ad element in soap advertisement on Femina magazine period of Jauari 2014- August 2016. Method of this research using quantitative descriptive content analysis. Total object of this research are 27 advertisement. The result of this research showing that domination of using headline in advertisement is provocative headline with percentage 44,44%, advertisement tend to not using subheadline with percentage 51,85%, domination of body copy in advertisement is use picture- caption copy with percentage 48,15%, advertisement tend to using photography ilustration technique with percentage 96,29% and domination of appealing point is using informational appealing point with percentage 48,15%. Salah satu media massa yang terdapat di Indonesia yang digunakan untuk sarana beriklan adalah majalah wanita Femina. Karena target pembaca Femina adalah untuk wanita, maka Femina adalah majalah berpotensial bagi para produk kecantikan dan toiletries untuk beriklan. Salah satu produk toiletries adalah sabun mandi. Dalam penulisan iklan (print ad memiliki elemen yang terdiri dari headline, subheadline, body copy dan ilustrasi serta mengandung suatu daya tarik agar menarik perhatian pembaca yang terdiri dari daya tarik rasional, emosional dan kombinasi. Tujuan penelitian adalah untuk mengetahui dominasi daya tarik pesan berdasarkan elemen print ad pada iklan produk sabun mandi di majalah Femina periode

  13. PEMERINTAH KABUPATEN MUSI RAWAS PROVINSI SUMATERA SELATAN BERDASARKAN HASIL PEMERIKSAAN BPK-RI ATAS LAPORAN KEUANGAN PEMERINTAH DAERAH TAHUN 2015

    Directory of Open Access Journals (Sweden)

    Tevi Leviany

    2016-04-01

    Pengecualian (Qualified Opinion. Tulisan ini bermaksud melakukan kajian yang lebih mendalam terhadap Laporan Hasil Pemeriksaan (LHP atas Laporan Keuangan Pemerintahan Daerah  Kabupaten Musi Rawas dengan menggunakan analisis isi untuk memperoleh gambaran yang lebih lengkap mengenai kondisi akuntabilitas keuangan pemerintah Kabupaten Musi Rawas, khususnya dilihat dari sisi efektivitas sistem pengendalian intern. Berdasarkan hasil analisis disimpulkan bahwa permasalahan efektivitas sistem pengendalian intern pemerintah kabupaten Musi Rawas paling banyak dijukmpai terkait kelemahan komponen Lingkungan Pengendalian serta pada komponen Penilaian Resiko dan Pemantauan. Hasil uji F Statistik sebesar 135,06 menunjukan bahwa secara keseluruhan perencanaan pajak berpengaruh terhadap nilai perusahaan, sehingga hipotesis awal Ho penelitian ini. Kata Kunci: Akuntabilitas Keuangan; Hasil Pemeriksaan; Sistem Pengendalian Intern.

  14. Deteksi Dini Kasus Demam Berdarah Dengue Berdasarkan Faktor Cuaca di DKI Jakarta Menggunakan Metode Zero Truncated Negative Binomial

    Directory of Open Access Journals (Sweden)

    Robert Kurniawan

    2017-11-01

    Full Text Available AbstractThe incidence rates of DHF in Jakarta in 2010 to 2014 are always higher than that of the national rates. Therefore, this study aims to find the effect of weather parameter on DHF cases. Weather is chosen because it can be observed daily and can be predicted so that it can be used as earlydetection in estimating the number of DHF cases. Data use includes DHF cases which is collected daily and weather data including lowest and highest temperatures and rainfall. Data analysis used is zero-truncated negative binomial analysis at 10% significance level. Based on the periodic dataof selected variables from January 1st 2015 until May 31st 2015, the study revealed that weather factors consisting of highest temperature, lowest temperature, and rainfall rate were significant enough to predict the number of DHF patients in DKI Jakarta. The three variables had positiveeffects in influencing the number of DHF patients in the same period. However, the weather factors cannot be controlled by humans, so that appropriate preventions are required whenever weather’s predictions indicate the increasing number of DHF cases in DKI Jakarta.Keywords: Dengue Hemorrhagic Fever, zero truncated negative binomial, early warning.AbstrakAngka kesakitan DBD pada tahun 2010 hingga 2014 selalu lebih tinggi dibandingkan dengan angka kesakitan DBD nasional. Oleh karena itu, penelitian ini bertujuan untuk mencari pengaruh faktor cuaca terhadap kasus DBD. Faktor cuaca dipilih karena dapat diamati setiap harinya dan dapat diprediksi sehingga dapat dijadikan deteksi dini dalam perkiraan jumlah penderita DBD. Data yang digunakan dalam penelitian ini adalah data jumlah penderita DBD di DKI Jakarta per hari dan data cuaca yang meliputi suhu terendah, suhu tertinggi dan curah hujan. Untuk mengetahui pengaruh faktor cuaca tersebut terhadap jumlah penderita DBD di DKI Jakarta digunakan metode analisis zero-truncated negative binomial. Berdasarkan data periode 1 Januari 2015 hingga

  15. Hubungan Kadar FT4 dengan Gejala Klinis yang Terkait Efek Simpatis berdasarkan Indek Wayne pada Nagari Koto Salak Kabupaten Dharmasraya

    Directory of Open Access Journals (Sweden)

    Nining Kurniawati

    2015-05-01

    Full Text Available AbstrakPeningkatan aktivitas saraf simpatis dapat terjadi pada keadaan hipertiroid dan sebaliknya pada hipotiroid. Pengukuran kadar hormon tiroid dilakukan dengan mengukur kadar FT4, FT3, TSH, dll. Tujuan penelitian ini adalah untuk mengidentifikasi hubungan kadar FT4 dengan gejala klinis yang terkait efek simpatis di Nagari Koto Salak Kabupaten Dharmasraya. Penelitian ini menggunakan desain penelitian cross sectional study. Hasil penelitian didapatkan subjek dengan peningkatan kadar FT4 12,96%. Subjek dengan gejala klinis palpitasi, penurunan berat badan, nervous, berkeringat lebih dan tremor jari halus dengan persentase berturut-turut adalah 42,59%, 38,89%, 46,30%, 25,93% dan 44,44%. Analisis bivariat dengan menggunakan uji statistik chi-square diperoleh hasil tidak ada hubungan hubungan kadar FT4 dengan 5 gejala klinis yang terkait efek simpatis berdasarkan indeks Wayne (p > 0.05. Penelitian ini masih sederhana dan belum bisa menunjukkan adanya hubungan antara kadar FT4 dengan gejala klinis yang terkait efek simpatis. Sebaiknya untuk penelitian yang akan datang diharapkan dapat memiliki jumlah sampel yang banyak dan cakupan gejala klinis lain yang terlibat dalam aktivitas saraf simpatis yang lebih luas sehingga dapat lebih lengkap dan spesifik.Kata kunci: Kadar FT4, efek simpatis, gejala klinis AbstractIncreased sympathetic nerve activity may occur in the state of hyperthyroidism and conversely in hypothyroidism. Measurement of thyroid hormone levels is done by measuring the levels of FT4, FT3, TSH, etc. The objective of this study was to identify the relation between FT4 levels and the clinical symptoms of sympathetic effects in Nagari Koto Salak Kabupaten Dharmasraya. This research used cross sectional study design. According to the research found that subjects with elevated levels of FT4 12.96%. There are subjects with clinical symptoms of palpitations, weight loss, nervousness, sweating more and fine finger tremor by the following

  16. Sobre el iuspositivismo que hemos de dejar atrás. Una crítica iusnaturalista a "Dejemos atrás el positivismo jurídico" de Atienza y de Ruiz Manero

    Directory of Open Access Journals (Sweden)

    JOSÉ CHÁVEZ-FERNÁNDEZ

    2011-01-01

    Full Text Available A través de la confrontación de las tesis más relevantes que Atienza y Ruiz Manero proponen en su artículo "Dejemos atrás el positivismo jurídico", el autor pretende mostrar-a partir de la respuesta a las preguntas "¿qué iuspositivismo hemos de dejar atrás?", "¿por qué?" y "¿cómo?"- que existen en el iuspositivismo debilidades explicativas graves respecto de la realidad jurídica, y que estas están vinculadas tanto al escepticismo ético como a la negación de la existencia de un Derecho natural en sentido propio, es decir, de raigambre antropológico-metafísica. Por otro lado, se tratará de evidenciar que dichas debilidades explicativas le son comunes también a los intentos pragmático-constructivistas de superación del iuspositivismo por lo que, en última instancia, ninguno de dichos esquemas conceptuales puede dar cuenta cabal de una realidad como los derechos humanos que se presentan como deudas ético-jurídicas inexcusables fundamentadas en la dignidad ontológica de todos los seres humanos.

  17. Klasifikasi Dokumen Karya Akhir Mahasiswa Menggunakan Naïve Bayes Classifier (NBC Berdasarkan Abstrak Karya Akhir Di Jurusan Teknik Elektro Universitas Negeri Jakarta

    Directory of Open Access Journals (Sweden)

    Nur Indah Pratiwi

    2017-06-01

    Full Text Available Dokumen karya akhir di Jurusan Teknik Elektro Universitas Negeri Jakarta setiap tahunnya bertambah, pengklasifikasian dokumen menjadi hal yang sangat penting untuk mengorganisasikan dokumen sehingga dapat memudahkan pencarian. Pengembangan Sistem klasifikasi dokumen bertujuan untuk mengembangkan sebuah sistem yang dapat mengklasifikasikan dokumen karya akhir mahasiswa berdasarkan abstrak karya akhir menggunakan algoritma Naïve Bayes Classifier (NBC. Sehingga, dapat memudahkan pengklasifikasian dokumen karya akhir di Jurusan Teknik Elektro. Dalam penelitian ini menggunakan metode eksperimen dan menggunakan 100 dokumen abstrak, 90 dokumen sebagai data train dan 10 dokumen sebagai data test. Data diambil dari skripsi mahasiswa Jurusan Teknik Elektro Universitas Negeri Jakarta dari 14 Maret 2014 sampai dengan 27 Maret 2014. Setelah melakukan proses pengembangan perangkat lunak, dihasilkan sebuah sistem klasifikasi yang bernama Sistem Klasifikasi Dokumen Skripsi. Sistem di implementasi menggunakan PHP dan MySQL, dan diuji menggunakan K-Fold Cross Validation (10 Fold. Berdasarkan pada hasil uji Sistem didapatkan hasil tingkat akurasi sebesar 81%. Oleh karena itu, dapat disimpulkan bahwa Sistem Klasifikasi Dokumen Abstrak Karya Akhir Menggunakan Algoritma Naïve Bayes di Jurusan Teknik Elektro telah berhasil dikembangkan.

  18. Kajian Pemilihan Sumber Mikroorganisme Solid Phase Microbial Fuel Cell (SMFC Berdasarkan Jenis dan Volume Sampah, Power Density dan Efisiensi Penurunan COD

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    Ganjar Samudro

    2017-06-01

    Full Text Available Mikroorganisme merupakan salah satu komponen penting dalam proses Solid Phase Microbial Fuel Cell (SMFC untuk degradasi bahan organik dan transfer elektron. Pemilihan sumber mikroorganisme menjadi metode yang paling sederhana untuk dikaji sebagai informasi awal ketersediaan dan identifikasi jenis mikroorganisme yang mendukung proses SMFC. Tujuan kajian ini adalah untuk memilih sumber mikroorganisme tanah, septic tank dan sedimen sungai yang tepat digunakan dalam proses SMFC berdasarkan jenis dan volume sampah, power density, dan efisiensi penurunan COD. Kajian ini didasarkan pada hasil penelitian menggunakan reaktor SMFC tipe single chamber microbial fuel cell dengan variabel jenis dan volume sampah , serta sumber mikroorganisme. Metode perbandingan secara kuantitatif dilakukan berdasarkan kecenderungan nilai power density dan efisiensi penurunan COD tertinggi di antara jenis dan volume sampah kantin, dedaunan dan komposit kantin-dedaunan. Hasil yang didapatkan adalah sumber mikroorganisme tanah dan sedimen sungai tepat digunakan untuk volume sampah 1/3 dan 2/3 dari volume reaktor, sedangkan sumber mikroorganisme septic tank tepat digunakan untuk volume sampah 1/3 dan 1/2 dari volume reaktor. Sumber mikroorganisme dari septic tank menunjukkan kinerja power density dan efisiensi penurunan COD yang lebih rendah dibandingkan sumber mikroorganisme tanah dan sedimen sungai.

  19. Evaluasi Manajemen Keamanan Informasi Menggunakan Indeks Keamanan Informasi (KAMI Berdasarkan ISO/IEC 27001:2013 pada Direktorat Pengembangan Teknologi dan Sistem Informasi (DPTSI ITS Surabaya

    Directory of Open Access Journals (Sweden)

    Firzah Abdullah Basyarahil

    2017-03-01

    Full Text Available DPTSI merupakan sebuah direktorat untuk menangani permasalahan teknologi informasi dan sistem informasi yang dimiliki oleh ITS. Menurut UU. No. 12 Tahun 12 Ttg. Perguruan Tinggi, misi mencari, menemukan, dan menyebarluaskan kebenaran ilmiah tersebut dapat diwujudkan apabila perguruan tinggi di kelola berdasarkan suatu Tata kelola perguruan tinggi yang baik (Good University Governance. Pengelolaan Informasi merupakan salah satu aspek dalam Good University Governance, termasuk kualitas dan keamanan pengelolaan informasi. Salah satu upaya yang dapat dilakukan untuk meningkatkan kualitas dari keamanan informasi, kementrian Kominfo membuat alat bantu untuk mengukur tingkat kematangan dan kelengkapan dalam keamanan informasi yang disebut dengan Indeks Keamanan Informasi (KAMI. Penggunaan Indeks KAMI ini juga diikuti dengan penerapan ISO 27001 sebagai standar keamanan internasional yang dapat membantu sebuah organisasi memastikan bahwa keamanan informasi yang diterapkan sudah efektif. Hasil dari penggunaan Indeks KAMI versi 3.1 di DPTSI ITS ini adalah tingkat ketergantungan penggunaan sistem elektronik sebesar 26 dari total skor 50 dan masuk kedalam kategori Tinggi dimana sistem elektronik adalah bagian yang tidak terpisahkan dari proses kerja yang berjalan. Hasil penilaian kelima area yang telah dilakukan adalah sebesar 249 dari 645 dan berada pada kategori tidak layak. Dari hasil tersebut maka dibuat rekomendasi berdasarkan kontrol ISO 27002:2013 untuk pertanyaan-pertanyaan yang mendapat nilai kurang. Kemudian rekomendasi dari penelitian ini dapat dijadikan sebagai bahan pertimbangan dan evaluasi bagi pihak DPTSI ITS Surabaya dalam melakukan perbaikan yang berkaitan dengan mitigasi atau pencegahan kerentanan keamanan informasi, serta memastikan regulasi dapat dicapai dengan baik dan kebijakan keamanan institusi di masa yang akan datang.

  20. In-situ monitoring of blood glucose level for dialysis machine by AAA-battery-size ATR Fourier spectroscopy

    Science.gov (United States)

    Hosono, Satsuki; Sato, Shun; Ishida, Akane; Suzuki, Yo; Inohara, Daichi; Nogo, Kosuke; Abeygunawardhana, Pradeep K.; Suzuki, Satoru; Nishiyama, Akira; Wada, Kenji; Ishimaru, Ichiro

    2015-07-01

    For blood glucose level measurement of dialysis machines, we proposed AAA-battery-size ATR (Attenuated total reflection) Fourier spectroscopy in middle infrared light region. The proposed one-shot Fourier spectroscopic imaging is a near-common path and spatial phase-shift interferometer with high time resolution. Because numerous number of spectral data that is 60 (= camera frame rare e.g. 60[Hz]) multiplied by pixel number could be obtained in 1[sec.], statistical-averaging improvement realize high-accurate spectral measurement. We evaluated the quantitative accuracy of our proposed method for measuring glucose concentration in near-infrared light region with liquid cells. We confirmed that absorbance at 1600[nm] had high correlations with glucose concentrations (correlation coefficient: 0.92). But to measure whole-blood, complex light phenomenon caused from red blood cells, that is scattering and multiple reflection or so, deteriorate spectral data. Thus, we also proposed the ultrasound-assisted spectroscopic imaging that traps particles at standing-wave node. Thus, if ATR prism is oscillated mechanically, anti-node area is generated around evanescent light field on prism surface. By elimination complex light phenomenon of red blood cells, glucose concentration in whole-blood will be quantify with high accuracy. In this report, we successfully trapped red blood cells in normal saline solution with ultrasonic standing wave (frequency: 2[MHz]).

  1. An ATR-dependent function for the Ddx19 RNA helicase in nuclear R-loop metabolism.

    Science.gov (United States)

    Hodroj, Dana; Recolin, Bénédicte; Serhal, Kamar; Martinez, Susan; Tsanov, Nikolay; Abou Merhi, Raghida; Maiorano, Domenico

    2017-05-02

    Coordination between transcription and replication is crucial in the maintenance of genome integrity. Disturbance of these processes leads to accumulation of aberrant DNA:RNA hybrids (R-loops) that, if unresolved, generate DNA damage and genomic instability. Here we report a novel, unexpected role for the nucleopore-associated mRNA export factor Ddx19 in removing nuclear R-loops formed upon replication stress or DNA damage. We show, in live cells, that Ddx19 transiently relocalizes from the nucleopore to the nucleus upon DNA damage, in an ATR/Chk1-dependent manner, and that Ddx19 nuclear relocalization is required to clear R-loops. Ddx19 depletion induces R-loop accumulation, proliferation-dependent DNA damage and defects in replication fork progression. Further, we show that Ddx19 resolves R-loops in vitro via its helicase activity. Furthermore, mutation of a residue phosphorylated by Chk1 in Ddx19 disrupts its interaction with Nup214 and allows its nuclear relocalization. Finally, we show that Ddx19 operates in resolving R-loops independently of the RNA helicase senataxin. Altogether these observations put forward a novel, ATR-dependent function for Ddx19 in R-loop metabolism to preserve genome integrity in mammalian cells. © 2017 The Authors.

  2. Galiellalactone induces cell cycle arrest and apoptosis through the ATM/ATR pathway in prostate cancer cells.

    Science.gov (United States)

    García, Víctor; Lara-Chica, Maribel; Cantarero, Irene; Sterner, Olov; Calzado, Marco A; Muñoz, Eduardo

    2016-01-26

    Galiellalactone (GL) is a fungal metabolite that presents antitumor activities on prostate cancer in vitro and in vivo. In this study we show that GL induced cell cycle arrest in G2/M phase, caspase-dependent apoptosis and also affected the microtubule organization and migration ability in DU145 cells. GL did not induce double strand DNA break but activated the ATR and ATM-mediated DNA damage response (DDR) inducing CHK1, H2AX phosphorylation (fH2AX) and CDC25C downregulation. Inhibition of the ATM/ATR activation with caffeine reverted GL-induced G2/M cell cycle arrest, apoptosis and DNA damage measured by fH2AX. In contrast, UCN-01, a CHK1 inhibitor, prevented GL-induced cell cycle arrest but enhanced apoptosis in DU145 cells. Furthermore, we found that GL did not increase the levels of intracellular ROS, but the antioxidant N-acetylcysteine (NAC) completely prevented the effects of GL on fH2AX, G2/M cell cycle arrest and apoptosis. In contrast to NAC, other antioxidants such as ambroxol and EGCG did not interfere with the activity of GL on cell cycle. GL significantly suppressed DU145 xenograft growth in vivo and induced the expression of fH2AX in the tumors. These findings identify for the first time that GL activates DDR in prostate cancer.

  3. Major Roles for Pyrimidine Dimers, Nucleotide Excision Repair, and ATR in the Alternative Splicing Response to UV Irradiation

    Directory of Open Access Journals (Sweden)

    Manuel J. Muñoz

    2017-03-01

    Full Text Available We have previously found that UV irradiation promotes RNA polymerase II (RNAPII hyperphosphorylation and subsequent changes in alternative splicing (AS. We show now that UV-induced DNA damage is not only necessary but sufficient to trigger the AS response and that photolyase-mediated removal of the most abundant class of pyrimidine dimers (PDs abrogates the global response to UV. We demonstrate that, in keratinocytes, RNAPII is the target, but not a sensor, of the signaling cascade initiated by PDs. The UV effect is enhanced by inhibition of gap-filling DNA synthesis, the last step in the nucleotide excision repair pathway (NER, and reduced by the absence of XPE, the main NER sensor of PDs. The mechanism involves activation of the protein kinase ATR that mediates the UV-induced RNAPII hyperphosphorylation. Our results define the sequence UV-PDs-NER-ATR-RNAPII-AS as a pathway linking DNA damage repair to the control of both RNAPII phosphorylation and AS regulation.

  4. Department of Energy's Advanced Test Reactor (ATR), July 14--18, 1980: An independent on-site safety review

    International Nuclear Information System (INIS)

    1981-02-01

    The intent of this review was not to conduct a detailed in-depth audit, but rather to make a broad management assessment of ATR operations. The results of the review should only be considered as having identified trends or indications. The Team's observations and recommendations for the most part are based upon standards used for licensed reactor facility practices. These standards form the basis for many of the comments in this report. The Team believes that a uniform minimum standard of performance should be achieved in the operation of DOE reactors. In order to assure that this is accomplished, clear standards are necessary. Consistent with the past AEC and ERDA policy, the Team has used the standards of the commercial nuclear power industry. It is recognized that this approach is conservative, in that the ATR reactor has a significantly greater degree of inherent safety (lower pressure, temperature, power, etc.) than a licensed reactor. Although the Review Team found no indications or evidence that the plant is being operated in an unsafe manner, various areas were identified where improvements are either needed or should be considered to increase the safety of reactor operations

  5. A new integrated TLC/MU-ATR/SERS advanced approach for the identification of trace amounts of dyes in mixtures.

    Science.gov (United States)

    Sciutto, Giorgia; Prati, Silvia; Bonacini, Irene; Litti, Lucio; Meneghetti, Moreno; Mazzeo, Rocco

    2017-10-23

    The present research is focused on the setting up of an advanced analytical system for the detection of synthetic dyes. The system is based on the combination of an innovative thin layer chromatography (TLC) plate coupled with enhanced infrared (MU-ATR, metal underlayer attenuated total reflection) and Surface Enhanced Raman (SERS) spectroscopy. In particular, a TLC plate made of silver iodide (AgI) applied onto a gold coated glass slide (AgI@Au) is proposed as an efficient stationary phase for the separation of dyes mixtures. The separated dyes are then identified by means of both enhanced FTIR and SERS, performed directly on the same eluted spots. The use of a mid-IR transparent inorganic salt as stationary phase coupled with the underneath gold layer avoids spectral interferences, enhancing the signal obtained from ATR analyses. At the same time, SERS spectra can be recorded as the TLC plate may act as a SERS active substrate due to the photoreduction of AgI to metallic Ag caused by the exposure to the laser during the Raman analysis. Different mixtures of synthetic dyes of known composition, widely used in dyeing processes, have been tested and the method resulted to be effective in identifying trace amounts in the order of tens nanograms. Moreover, the method has been further evaluated on a real case study represented by dyes extracted from dyed wool. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. ATR FT-IR spectroscopy on Vmh2 hydrophobin self-assembled layers for Teflon membrane bio-functionalization

    International Nuclear Information System (INIS)

    Portaccio, M.; Gravagnuolo, A.M.; Longobardi, S.; Giardina, P.; Rea, I.; De Stefano, L.; Cammarota, M.; Lepore, M.

    2015-01-01

    Graphical abstract: - Highlights: • Hydrophobin self-assembled layers on Teflon in different preparation conditions were investigated. • ATR collection data geometry allowed samples examination without any particular preparation. • Amide content, lipid/amide and carbohydrate/amide ratios of the protein layer were estimated. • Secondary structure of protein was determined for the examined samples. • FT-IR demonstrated to be of extreme relevance in monitoring hydrophobin self-assembled layers preparation. - Abstract: Surface functionalization by layers of hydrophobins, amphiphilic proteins produced by fungi offers a promising and green strategy for fabrication of biomedical and bioanalytical devices. The layering process of the Vmh2 hydrophobin from Pleurotus ostreatus on Teflon membrane has been investigated by Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) spectroscopy. In particular, protein layers obtained with hydrophobin purified with two different procedures and in various coating conditions have been examined. The layers have been characterized by quantifying the amide I and amide II band area together with the lipid/amide ratio and carbohydrate/amide ratio. This characterization can be very useful in evaluating the best purification strategy and coating conditions. Moreover the analysis of the secondary structure of the layered protein using the deconvolution procedure of amide I band indicate the prevalent contribution from β-sheet state. The results inferred by infrared spectroscopy have been also confirmed by scanning electron microscopy imaging

  7. Characterization of Satsuma mandarin (Citrus unshiu Marc.) nectar-to-honey transformation pathway using FTIR-ATR spectroscopy.

    Science.gov (United States)

    Svečnjak, Lidija; Prđun, Saša; Rogina, Josip; Bubalo, Dragan; Jerković, Igor

    2017-10-01

    Samples of Satsuma mandarin (Citrus unshiu Marc.) nectar, honey sac content and honey were analyzed by FTIR-ATR spectroscopy and reference methods. The spectral analysis allowed detection of the major chemical constituents in C. unshiu nectar-to-honey transformation pathway thus providing information on the intensity and location of the compositional changes occurring during this process. The preliminary results showed that in average more than one-third of sugar-related nectar-to-honey conversion takes place directly in the honey sac; the average sugar content (w/w) was 17.93% (nectar), 47.03% (honey sac) and 79.63% (honey). FTIR-ATR results showed great spectral similarity of analyzed honey samples and small degree variations in both sugar and water content in nectar samples. The spectral data revealed distinctive differences in the chemical composition of individual honey sac contents with the most intensive and complex absorption envelope in the spectral region between 1175 and 950cm -1 (glucose, fructose and sucrose absorption bands). Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. Effect of dissolved oxygen on two bacterial pathogens examined using ATR-FTIR spectroscopy, microelectrophoresis, and potentiometric titration.

    Science.gov (United States)

    Castro, Felipe D; Sedman, Jacqueline; Ismail, Ashraf A; Asadishad, Bahareh; Tufenkji, Nathalie

    2010-06-01

    The effects of dissolved oxygen tension during bacterial growth and acclimation on the cell surface properties and biochemical composition of the bacterial pathogens Escherichia coli O157:H7 and Yersinia enterocolitica are characterized. Three experimental techniques are used in an effort to understand the influence of bacterial growth and acclimation conditions on cell surface charge and the composition of the bacterial cell: (i) electrophoretic mobility measurements; (ii) potentiometric titration; and (iii) ATR-FTIR spectroscopy. Potentiometric titration data analyzed using chemical speciation software are related to measured electrophoretic mobilities at the pH of interest. Titration of bacterial cells is used to identify the major proton-active functional groups and the overall concentration of these cell surface ligands at the cell membrane. Analysis of titration data shows notable differences between strains and conditions, confirming the appropriateness of this tool for an overall charge characterization. ATR-FTIR spectroscopy of whole cells is used to further characterize the bacterial biochemical composition and macromolecular structures that might be involved in the development of the net surficial charge of the organisms examined. The evaluation of the integrated intensities of HPO(2)(-) and carbohydrate absorption bands in the IR spectra reveals clear differences between growth protocols. Taken together, the three techniques seem to indicate that the dissolved oxygen tension during cell growth or acclimation can noticeably influence the expression of cell surface molecules and the measurable cell surface charge, though in a strain-dependent fashion.

  9. ATR FT-IR spectroscopy on Vmh2 hydrophobin self-assembled layers for Teflon membrane bio-functionalization

    Energy Technology Data Exchange (ETDEWEB)

    Portaccio, M., E-mail: marianna.portaccio@unina2.it [Dipartimento di Medicina Sperimentale – Seconda Università di Napoli, Via S.M. di Costantinopoli, 16-80134 Napoli (Italy); Gravagnuolo, A.M., E-mail: alfredomaria.gravagnuolo@unina.it [Dipartimento di Scienze Chimiche, Università “Federico II”, Via Cintia, 21- 80126 Napoli (Italy); Longobardi, S., E-mail: sara.longobardi@unina.it [Dipartimento di Scienze Chimiche, Università “Federico II”, Via Cintia, 21- 80126 Napoli (Italy); Giardina, P., E-mail: paola.giardina@unina.it [Dipartimento di Scienze Chimiche, Università “Federico II”, Via Cintia, 21- 80126 Napoli (Italy); Rea, I., E-mail: ilaria.rea@na.imm.cnr.it [Institute for Microelectronics and Microsystems, CNR, Via P. Castellino, 111-80131 Napoli (Italy); De Stefano, L., E-mail: luca.destefano@na.imm.cnr.it [Institute for Microelectronics and Microsystems, CNR, Via P. Castellino, 111-80131 Napoli (Italy); Cammarota, M., E-mail: marcella.cammarota@unina2.it [Dipartimento di Medicina Sperimentale – Seconda Università di Napoli, Via S.M. di Costantinopoli, 16-80134 Napoli (Italy); Lepore, M., E-mail: maria.lepore@unina2.it [Dipartimento di Medicina Sperimentale – Seconda Università di Napoli, Via S.M. di Costantinopoli, 16-80134 Napoli (Italy)

    2015-10-01

    Graphical abstract: - Highlights: • Hydrophobin self-assembled layers on Teflon in different preparation conditions were investigated. • ATR collection data geometry allowed samples examination without any particular preparation. • Amide content, lipid/amide and carbohydrate/amide ratios of the protein layer were estimated. • Secondary structure of protein was determined for the examined samples. • FT-IR demonstrated to be of extreme relevance in monitoring hydrophobin self-assembled layers preparation. - Abstract: Surface functionalization by layers of hydrophobins, amphiphilic proteins produced by fungi offers a promising and green strategy for fabrication of biomedical and bioanalytical devices. The layering process of the Vmh2 hydrophobin from Pleurotus ostreatus on Teflon membrane has been investigated by Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) spectroscopy. In particular, protein layers obtained with hydrophobin purified with two different procedures and in various coating conditions have been examined. The layers have been characterized by quantifying the amide I and amide II band area together with the lipid/amide ratio and carbohydrate/amide ratio. This characterization can be very useful in evaluating the best purification strategy and coating conditions. Moreover the analysis of the secondary structure of the layered protein using the deconvolution procedure of amide I band indicate the prevalent contribution from β-sheet state. The results inferred by infrared spectroscopy have been also confirmed by scanning electron microscopy imaging.

  10. In Vivo and In Vitro Effects of ATM/ATR Signaling Pathway on Proliferation, Apoptosis, and Radiosensitivity of Nasopharyngeal Carcinoma Cells.

    Science.gov (United States)

    Wang, Ming; Liu, Gang; Shan, Guo-Ping; Wang, Bing-Bing

    2017-08-01

    The study investigated the ability of ataxia-telangiectasia mutated (ATM)/Rad3-related (ATR) signaling pathway to influence the proliferation, apoptosis, and radiosensitivity of nasopharyngeal carcinoma (NPC) cells. NPC tissues and corresponding adjacent normal tissues were collected from 143 NPC patients. The NPC CNE2 cells were assigned into a control group, X-ray group, CGK-733 group, and X-ray+CGK-733 group. The mRNA levels of ATM and ATR were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) and the protein levels of ATM and ATR using western blotting. The positive expression of ATM and ATR in tissues and nude mouse tumor tissues was determined by immunohistochemistry. Cell proliferation, migration, invasion, and apoptosis rates were analyzed by the 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide (MTT) assay, scratch test, transwell assay, and flow cytometry, respectively. A nude mouse model of NPC was established to observe tumor volume and growth. The mRNA levels of ATR and ATM and the expression of ATR and ATM protein in NPC tissues were significantly higher than those in adjacent normal tissues. The colony formation assay showed that the colony-forming rate decreased, showing radiation dose-dependent and CGK-733 concentration-dependent manners. Expression of ATM, ATR, Chk1, and Chk2 was evidently increased in the X-ray, CGK-733, and X-ray+CGK-733groups compared with the control group, and the aforementioned expression was highest in the X-ray+CGK-733 group among the four groups. The cell proliferation, invasion, and migration were decreased, tumor volume decreased and cell apoptosis increased in the X-ray, CGK-733, and X-ray+CGK-733 groups compared with the control group; the X-ray+CGK-733 group exhibited lowest cell proliferation, invasion and migration, smallest tumor volume, and highest cell apoptosis among the four groups. Inhibition of ATM/ATR signaling pathway reduces proliferation and enhances apoptosis and

  11. Structure-Based Drug Design of Novel Potent and Selective Tetrahydropyrazolo[1,5- a ]pyrazines as ATR Inhibitors

    Energy Technology Data Exchange (ETDEWEB)

    Barsanti, Paul A; Aversa, Robert J.; Jin, Xianming; Pan, Yue; Lu, Yipin; Elling, Robert; Jain, Rama; Knapp, Mark; Lan, Jiong; Lin, Xiaodong; Rudewicz, Patrick; Sim, Janet; Taricani, Lorena; Thomas, George; Xiao, Linda; Yue, Qin (Novartis)

    2015-01-08

    A saturation strategy focused on improving the selectivity and physicochemical properties of ATR inhibitor HTS hit 1 led to a novel series of highly potent and selective tetrahydropyrazolo[1,5-a]pyrazines. Use of PI3Kα mutants as ATR crystal structure surrogates was instrumental in providing cocrystal structures to guide the medicinal chemistry designs. Detailed DMPK studies involving cyanide and GSH as trapping agents during microsomal incubations, in addition to deuterium-labeled compounds as mechanistic probes uncovered the molecular basis for the observed CYP3A4 TDI in the series.

  12. Development of C-lignin with G/S-lignin and lipids in orchid seed coats – an unexpected diversity exposed by ATR-FT-IR spectroscopy

    DEFF Research Database (Denmark)

    Barsberg, Søren Talbro; Lee, Y.-I.; Rasmussen, Hanne Nina

    2018-01-01

    Cite this article: Barsberg ST, Lee Y-I, Rasmussen HN. Development of C-lignin with G/S-lignin and lipids in orchid seed coats – an unexpected diversity exposed by ATR-FT-IR spectroscopy. Seed Science Research https:// doi.org/10.1017/S0960258517000344......Cite this article: Barsberg ST, Lee Y-I, Rasmussen HN. Development of C-lignin with G/S-lignin and lipids in orchid seed coats – an unexpected diversity exposed by ATR-FT-IR spectroscopy. Seed Science Research https:// doi.org/10.1017/S0960258517000344...

  13. Hasil skrining berdasarkan metode MNA (mini nutritional assestment tidak berpengaruh terhadap lama rawat inap dan status pulang pasien lanjut usia di RSUP Dr. Sardjito Yogyakarta

    Directory of Open Access Journals (Sweden)

    Wahyu Hardi Prasetyo

    2016-08-01

    of result screening during initial hospitalization to discharge status of eldery patients based on MNA method was RR 1.29. This indicated that malnourished patient were at risk for uncovered discharged as much as 1.29 greater than those not malnourhized.Conclusions: There was no impact of screening result in admission to length of stay. There was impact of nutritional status to length of discharged status.KEYWORDS: discharge home, length of stay, nutritional status in initial admission.ABSTRAKLatar belakang: Pertumbuhan penduduk lanjut usia (umur ≥60 tahun meningkat secara cepat pada abad 21 ini, yang pada 2000 di seluruh dunia telah mencapai 425 juta jiwa (± 6,8%. Jumlah ini diperkirakan akan mengalami peningkatan hampir dua kali lipat pada 2025. Di Indonesia, persentase lanjut usia pada 1995 mencapai 7,5%. Dengan meningkatnya angka harapan hidup, jumlah lanjut usia pun akan bertambah banyak. Hal ini terkait dengan perlunya peningkatan pelayanan kesehatan lanjut usia. Pada lanjut usia, masalah gizi erat kaitannya dengan penyakit. Salah satu faktor yang menyebabkan lanjut usia menjadi rawan gizi yaitu peningkatan morbiditas penyakit. Dengan meningkatnya risiko penyakit dan disertai gangguan nutrisi pada lanjut usia, perlu dilakukan identifikasi risiko malnutrisi pada lanjut usia sedini mungkin. Penilaian status gizi awal pasien masuk rumah sakit sangat penting dilakukan secara rutin karena dapat menggambarkan status gizi pasien saat itu, mendeteksi pasien-pasien yang berisiko tinggi, dan membantu mengidentifikasi perawatan gizi secara spesifik pada masing-masing pasien sehingga dukungan nutrisi yang tepat dapat diterapkan untuk meningkatan status gizi pasien.Tujuan: Untuk mengetahui pengaruh hasil skrining awal berdasarkan metode MNA (mini nutritional assessment terhadap lama rawat inap dan status pulang pasien lanjut pada ruang rawat inap penyakit dalam dan saraf di RSUP Dr.Sardjito Yogyakarta.Metode: Jenis penelitian ini adalah penelitian observasional

  14. Kualitas Air Sumur Gali Kelurahan Lubuk Buaya Kecamatan Koto Tangah Kota Padang Berdasarkan Indeks Most Probable Number (MPN

    Directory of Open Access Journals (Sweden)

    Randa Novalino

    2016-09-01

    Full Text Available AbstrakDiare merupakan salah satu penyakit yang ditularkan melalui air terkontaminasi oleh agen penyebab seperti bakteri Coliform.  Menurut data Dinas Kesehatan Kota (DKK Padang pada tahun 2011, kejadian diare di Kelurahan Lubuk Buaya Kecamatan Koto Tangah, Kota Padang merupakan kasus tertinggi di Kota Padang. Tujuan penelitian ini adalah menentukan kualitas air sumur gali di Kelurahan Lubuk Buaya berdasarkan Indeks Most Probable Number (MPN menurut Peraturan Menteri Kesehatan Republik Indonesia (Permenkes RI No. 416 tahun 1990. Sampel penelitian ini adalah  air sumur gali yang digunakan di beberapa Rukun Tetangga (RT, yang diambil secara acak dari beberapa Rukun Warga (RW yang telah dipilih sebelumnya, sehingga didapatkan 15 sampel. Penelitian ini dilaksanakan dalam 2 tahap yaitu pengambilan sampel air sumur gali sekaligus observasi faktor yang mempengaruhi kualitas air dan pemeriksaan mikrobiologi dengan metode Most Probable Number (MPN Test. Tes ini terdiri dari tes presumtif dan tes konfirmatif yang disesuaikan dengan Permenkes RI. Hasil penelitian ialah 73,33% dari jumlah sumur yang diperiksa tidak memenuhi standar Permenkes R.I. karena mengandung Coliform > 50 pada setiap 100 ml air. Hanya 26,6% sumur yang memenuhi standar yang telah ditetapkan. Beberapa faktor yang dapat mempengaruhi yaitu lokasi sumber pencemaran, dinding parapet, drainase, tutup sumur dan sarana pengambilan air.Kata kunci: kualitas air sumur gali, MPN, coliform AbstractDiarrhea is one of the diseases that  transmitted through contaminated water by causative agent, one of which is coliform bacteria. According to data from City Health Department Padang in 2011, the incidence of diarrhea in Kelurahan Lubuk Buaya Kecamatan Koto Tangah – Padang is the highest case in the city of Padang. The objective of this study was to determine the water quality of wells dug in Kelurahan Lubuk Buaya by Most Probable Number Index (MPN according regulation of Indonesian health

  15. Prediksi Risiko Karies Baru Berdasarkan Konsumsi Pempek pada Anak Usia 1112 Tahun Di Palembang (Tinjauan dengan Cariogram

    Directory of Open Access Journals (Sweden)

    Marlindayanti Marlindayanti

    2014-12-01

    Full Text Available Penyakit rongga mulut yang sering diderita anak adalah karies gigi. Hasil penelitian terdahulu menunjukkan prevalensi karies gigi anak di Palembang sebesar 92,43%. Pempek makanan khas jenis karbohidrat lengket yang dimakan bersama kuahnya (cuko, kebiasaan anak di Palembang mengkonsumsi pempek lebih dari 2 kali sehari. Frekuensi konsumsi karbohidrat yang sering berakibat karies gigi. Kebiasaan anak di Palembang mengkonsumsi pempek merupakan faktor risiko terjadinya karies gigi. Risiko karies gigi perlu diketahui untuk melihat kisaran karies baru yang dapat terjadi. Penelitian ini bertujuan memprediksi risiko terjadinya karies baru berdasarkan frekuensi konsumsi pempek di Palembang. Penelitian ini merupakan penelitian observasional dengan desain cross sectional. Pengambilan sampel menggunakan tekhnik cluster, subjek penelitian sebanyak 305 anak dari 52 SD di Palembang. Pengukuran prediksi risiko karies menggunakan cariogram dengan cara mengumpulkan data survei diet frekuensi konsumsi secara keseluruhan dan frekuensi konsumsi pempek, DMF-T, kapasitas buffer, sekresi saliva, plak skor, program fluor dan penyakit umum. Hasil penelitian menunjukkan prediksi risiko karies anak usia 11-12 tahun di Palembang 65,72% (kategori tinggi kontribusi pempek 45,83% dari total konsumsi makan keseluruhan. Peluang menghindari karies sebesar 34,28%. Urutan penyebab risiko karies adalah kerentanan (31,0%, pola makan (17,36%, bakteri (8,91% dan keadaan lain yang berpengaruh (5,35%. Kesimpulan penelitian, prediksi risiko terjadinya karies baru pada anak usia 11-12 tahun di Palembang termasuk kategori tinggi, pempek menyumbang 45,83% dari total konsumsi keseluruhan. Urutan prediksi risiko karies anak usia 11-12 tahun di Palembang, kerentanan, pola makan, bakteri dan faktor lain yang berpengaruh.    Prediction of The Risk Of New Caries Base on Pempek Consumption on Children Age 11-12 Years Old In Palembang. The oral cavity disease often suffered by children is dental

  16. JARINGAN SARAF TIRUAN DALAM MEMPREDIKSI SUKUK NEGARA RITEL BERDASARKAN KELOMPOK PROFESI DENGAN BACKPROPOGATION DALAM MENDORONG LAJU PERTUMBUHAN EKONOMI

    Directory of Open Access Journals (Sweden)

    Agus Perdana Windarto

    2017-09-01

    , yaitu Departemen Keuangan (depkeu. Dimana pemerintah akan memilih agen penjual dan konsultasi hukum sukuk ritel. Agen penjual haruslah wajib memiliki komitmen terhadap pemerintah dalam pengembangan pasar sukuk dan berpengalaman dalam menjual produk keuangan syariah. Penerbitan instrumen ini diibaratkan sebuah “simbiosis mutualis” antara Pemerintah dan Masyarakat, dimana keduanya sama-sama memperoleh keuntungan. Pemerintah selaku penerbit memperoleh keuntungan berupa  penggunaan dana dari masyarakat, sedangkan masyarakat memperoleh keuntungan dari investasi yang dilakukan. Penelitian ini memberikan kontribusi bagi pemerintah dan Bank untuk dapat melakukan promosi secara maksimal untuk penerbitat sukuk berikutnya. Data yang digunakan adalah data dari kemenkeu melalui website www.djppr.kemenkeu.go.id. Data tersebut adalah data penjualan sukuk dengan seri 001 – 007 yang dikelompokkan dalam beberapa kategori yakni geografis, profesi dan kategori umur. Algoritma yang digunakan pada penelitian ini adalah Jaringan Saraf Tiruan dengan metode Backpropogation. Variabel masukan (input yang digunakan adalah PNS (X1, Pegawai Swasta (X2, IRT (X3, Wiraswasta (X4, TNI/Polri (X5 dan Lainnya (X6 dengan model arsitektur pelatihan dan pengujian sebanyak 6 arsitektur yakni 6-2-1, 6-5-1, 6-2-5-1 dan 6-5-2-1. Keluaran (output yang dihasilkan adalah pola terbaik dari arsitektur JST. Model arsitektur terbaik adalah 6-5-2-1 dengan epoch 37535, MSE 0,0009997295 dan tingkat akurasi 100%. Dari model ini akan dilakukan analisis sensivitas untuk melihat variabel yang memiliki performa terbaik dan diperoleh variabel Pegawai Swasta (X2 dengan skor 0,3268. Sehingga didapat hasil prediksi investor terbanyak pada pembelian sukuk untuk seri 008 berikutnya berdasarkan kategori profesi adalah Pegawai Swasta. Kata Kunci: Sukuk, JST, Backpropogation, Analisis Sensivitas dan Prediksi

  17. Desain Pembelajaran Student’s Conceptual Construction Guider Berdasarkan Kesulitan Mahasiswa Calon Guru Fisika pada Konsep Gerak Parabola

    Directory of Open Access Journals (Sweden)

    Duden Saepuzaman

    2016-12-01

      dan y (vertikal. Kedua, membedakan vektor kecepatan, vektor komponen kecepatan, komponen vektor kecepatan dalam arah x dan y. Ketiga, terfokus pada hapalan rumus waktu yang ditempuh peluru untuk lintasan setengah parabola, tinggi maksimum,jarak terjauh maksimum. Keempat, selalu beranggapan kecepatan akhir benda yang bergerak parabola ketika menyentuh tanah selalu nol. Kelima, kecepatan benda nol di puncak (padahal hanya kecepatan dalam arah vertikal yang nol. Keenam, percepatan dimiliki oleh benda dalam arah x maupun y. Ketujuh, ditemukan beberapa mahasiswa menggunakan aturan sinus cosinus segitiga pada penentuan jarak/ ketinggian dan sebaliknya (padahal penentuan jarak/ketinggian berdasarkan informasi kecepatan. Diduga kuat, proses pembelajaran yang terjadi kurang  memfasilitasi  pengembangan kemampuan berpikir mahasiswa untuk membangun konsepnya sendiri (konstruktivisme. Penelitian ini bertujuan untuk membuat sebuah rancangan pembelajaran yang disebut  Student’s Conceptual Construction Guider. Metode yang digunakan adalah  metode analisis deskriftif. Pemahaman konseptual mahasiswa dievaluasi melalui tes konseptual yang berbentuk  uraian. Hasil penelitian  menunjukkan bahwa pembelajaran Student’s Conceptual Construction Guider dapat memfasilitasi  memfasilitasi  pengembangan kemampuan berpikir mahasiswa untuk membangun konsepnya sendiri  dalam memahami konsep gerak parabola. Kata-kata kunci: Student’s Conceptual Construction Guider , kesulitan mahasiswa , Gerak Parabola

  18. PENILAIAN KESESUAIAN PERAIRAN UNTUK BUDIDAYA IKAN DALAM KERAMBA JARING APUNG BERDASARKAN MODEL SPASIAL PROPAGASI OMBAK MENDEKATI PANTAI

    Directory of Open Access Journals (Sweden)

    Tarunamulia Tarunamulia

    2016-11-01

    Full Text Available Keberhasilan dan keberlanjutan usaha budidaya ikan dalam keramba jaring apung (KJA tidak terlepas dari tingkat keterlindungan lokasi dari aksi fisik peubah oseanografi fisik. Peubah oseanografi fisik yang paling utama menentukan tingkat keterlindungan lokasi adalah besar dan arah ombak yang datang ke pantai, karena selain berhubungan dengan tingkat kesesuaian lahan pada saat awal penyeleksian lokasi, juga dapat mempengaruhi aspek pengelolaan lebih lanjut setelah pelaksanaan dan pengembangan kegiatan budidaya. Penelitian ini bertujuan untuk menguraikan bentuk hubungan spasial antara perubahan iklim dengan kondisi oseanografi fisik khususnya peubah ombak dalam penilaian kesesuaian lahan untuk budidaya ikan dalam KJA. Penelitian ini menitikberatkan pada analisis spasial ombak untuk berbagai perubahan arah dan kecepatan angin yang mungkin terjadi pada lokasi penelitian. Model spasial tersebut dianalisis dengan memadukan metode analisis spasial dalam SIG dan metode penyelesaian mild-slope untuk model ombak permukaan di perairan pantai (CGWAVE. Evaluasi tingkat kesesuaian lahan dilakukan dengan melihat pengaruh ombak terhadap kondisi perairan yang sedang dan kemungkinan akan diperuntukkan untuk kegiatan budidaya ikan dalam KJA di wilayah pesisir Kabupaten Barru Provinsi Sulawesi Selatan. Model spasial ombak yang dikembangkan dalam penelitian ini menunjukkan bahwa perubahan iklim yang mempengaruhi kondisi ombak akan secara signifikan langsung maupun tidak langsung mempengaruhi status tingkat kesesuaian perairan untuk budidaya ikan dalam KJA di lokasi penelitian. Dari 9.939,0 ha total luas perairan yang layak menurut kriteria kedalaman di Kabupaten Barru, hanya tersisa sekitar 2,9% (296,8 ha yang layak setelah aspek keterlindungan berdasarkan ketinggian ombak diintegrasikan dalam analisis. Hasil penelitian ini pada akhirnya menyarankan perlunya pemahaman yang mendalam mengenai perubahan pola propagasi spasial ombak yang datang ke pantai akibat

  19. ANALISIS KELAYAKAN ISI BUKU TEKS MATA PELAJARAN EKONOMI SMA KELAS X BERDASARKAN KURIKULUM TINGKAT SATAUAN PENDIDIKAN ( KTSP DI KABUPATEN SEMARANG

    Directory of Open Access Journals (Sweden)

    Koeri Ulinuha

    2016-02-01

    Full Text Available Tujuan penelitian ini adalah untuk mengetahui apakah buku teks ekonomi SMA kelas X yang digunakan di Kabupaten Semarang sudah memenuhi standar kelayakan isi buku teks ekonomi menurut BSNP. Dan manfaat penelitian ini dapat memilih dan mempertimbankan dalam memilih buku teks pelajaran untuk refrensi belajar baik pihak siswa, guru dan pihak sekolahPenelitian ini dilakuakan terhadap isi buku teks pelajaran yang dianalisis secara kualitatif. Sehingga penelitian ini disebut penelitian deskriptif kualitatif. Teknik penelitian yang di gunakan adalah adalah teknik triangulasi. Analisis buku teks ekonomi untuk SMA kelas X melibatkan 5 orang penilai, yaitu 5 guru ekonomi. Dari 5 SMAN yang mengunakan buku teks pelajaran ekonomi dengan pengarang Alam S. dan penerbit Esis. Teknik pengumpulan data dengan lembar penilaian buku teks ekonomi dari BSNP dan menggunakan teknik wawancara terhadap dua guru ekonomi untuk memperkuat hasil dari penilaian dari buku teks ekonomi kelas X.Data yang diperoleh dalam penelitian ini berupa hasil penilaian yang berupa skor penilian dan hasil wawancara. Berdasarkan hasil analisis dapat disimpulkan bahwa buku ekonomi SMA Kelas X karangan Alam S. yang digunakan di Kabupaten Semarang sudah memenuhi standar kelayakan isi menurut BSNP. Buku teks ekonomi untuk SMA Kelas X karangan Alam S. dapat digunakan sebagai sumber belajar dalam pembelajaran ekonomi di SMA kelas X. Selain itu, perlu diadakan penelitian lebih lanjut untuk menganalisis buku teks ekonomi lain yang digunakan di Kabupaten Semarang.. The purpose of this research was to know whether textbooks high school economics class X used in Semarang Regancy already meet the standards of eligibility of the contents of the textbook economics according to BSNP. Analysis of economic text books for high school classes X involves 5 persons appraisers, i.e. 5 economics teacher. From 5 SMAN using textbook economics lessons with author and Publisher Alam S. Esis.. Data collection techniques

  20. Measured thermal and fast neutron fluence rates, ATR Cycle 100-BC, April 23, 1993--May 13, 1993

    International Nuclear Information System (INIS)

    Smith, L.D.; Murray, R.K.; Rogers, J.W.

    1993-07-01

    This report contains the thermal (2200 m/s) and fast (E>1MeV) neutron fluence rate data for ATR Cycle 100-BC which were measured by the Radiation Measurements Laboratory (RML) as requested by the Power Reactor Programs (ATR Experiments) Radiation Measurements Work Order. This report contains fluence rate values corresponding to the particular elevations (relative to the 80 ft. core elevation) where the measurements were taken. The data in this report consists of (1) a table of the ATR power history and distribution, (2) a hard copy listing of all thermal and fast neutron fluence rates, (3) plots of both the thermal and fast neutron fluence rates, and (4) a magnetic record (3.5 inch diskette) containing a listing of only the fast neutron fluence rates, their assigned elevations and proper header identification of all monitor positions contained herein. The fluence rates reported are for the average power levels given in the table of power history and distribution. All open-quotes Hclose quotes holder monitor wires for this cycle are 54 inches long. All open-quotes SRclose quotes holder monitor wires for this cycle are 55 inches long. This length allows measurement of the full core region and makes the first count elevation 24.73 inches above core midplane. Due to the safety rod problems in the west lobe, open-quotes BRclose quotes holders were used in the W-1, 2, 3, and 4 positions. All open-quotes BRclose quotes holder monitor wires for this cycle are 56.25 inches long. The distance from the end of the wires to the first count position was 4.25 inches for all wires counted from this cycle. The results from the measurements in the W-1, 2, 3, 4 monitor positions indicate that the safety rod followers were rotated to a different azimuthal orientation relative to the normal orientation. The results indicate that the rotation was counterclockwise from their normal orientation. This is the same condition observed starting with Cycle 99-B

  1. Quantitative analysis of glycated albumin in serum based on ATR-FTIR spectrum combined with SiPLS and SVM.

    Science.gov (United States)

    Li, Yuanpeng; Li, Fucui; Yang, Xinhao; Guo, Liu; Huang, Furong; Chen, Zhenqiang; Chen, Xingdan; Zheng, Shifu

    2018-08-05

    A rapid quantitative analysis model for determining the glycated albumin (GA) content based on Attenuated total reflectance (ATR)-Fourier transform infrared spectroscopy (FTIR) combining with linear SiPLS and nonlinear SVM has been developed. Firstly, the real GA content in human serum was determined by GA enzymatic method, meanwhile, the ATR-FTIR spectra of serum samples from the population of health examination were obtained. The spectral data of the whole spectra mid-infrared region (4000-600 cm -1 ) and GA's characteristic region (1800-800 cm -1 ) were used as the research object of quantitative analysis. Secondly, several preprocessing steps including first derivative, second derivative, variable standardization and spectral normalization, were performed. Lastly, quantitative analysis regression models were established by using SiPLS and SVM respectively. The SiPLS modeling results are as follows: root mean square error of cross validation (RMSECV T ) = 0.523 g/L, calibration coefficient (R C ) = 0.937, Root Mean Square Error of Prediction (RMSEP T ) = 0.787 g/L, and prediction coefficient (R P ) = 0.938. The SVM modeling results are as follows: RMSECV T  = 0.0048 g/L, R C  = 0.998, RMSEP T  = 0.442 g/L, and R p  = 0.916. The results indicated that the model performance was improved significantly after preprocessing and optimization of characteristic regions. While modeling performance of nonlinear SVM was considerably better than that of linear SiPLS. Hence, the quantitative analysis model for GA in human serum based on ATR-FTIR combined with SiPLS and SVM is effective. And it does not need sample preprocessing while being characterized by simple operations and high time efficiency, providing a rapid and accurate method for GA content determination. Copyright © 2018 Elsevier B.V. All rights reserved.

  2. Dynamic dependence on ATR and ATM for double-strand break repair in human embryonic stem cells and neural descendants.

    Directory of Open Access Journals (Sweden)

    Bret R Adams

    2010-04-01

    Full Text Available The DNA double-strand break (DSB is the most toxic form of DNA damage. Studies aimed at characterizing DNA repair during development suggest that homologous recombination repair (HRR is more critical in pluripotent cells compared to differentiated somatic cells in which nonhomologous end joining (NHEJ is dominant. We have characterized the DNA damage response (DDR and quality of DNA double-strand break (DSB repair in human embryonic stem cells (hESCs, and in vitro-derived neural cells. Resolution of ionizing radiation-induced foci (IRIF was used as a surrogate for DSB repair. The resolution of gamma-H2AX foci occurred at a slower rate in hESCs compared to neural progenitors (NPs and astrocytes perhaps reflective of more complex DSB repair in hESCs. In addition, the resolution of RAD51 foci, indicative of active homologous recombination repair (HRR, showed that hESCs as well as NPs have high capacity for HRR, whereas astrocytes do not. Importantly, the ATM kinase was shown to be critical for foci formation in astrocytes, but not in hESCs, suggesting that the DDR is different in these cells. Blocking the ATM kinase in astrocytes not only prevented the formation but also completely disassembled preformed repair foci. The ability of hESCs to form IRIF was abrogated with caffeine and siRNAs targeted against ATR, implicating that hESCs rely on ATR, rather than ATM for regulating DSB repair. This relationship dynamically changed as cells differentiated. Interestingly, while the inhibition of the DNA-PKcs kinase (and presumably non-homologous endjoining [NHEJ] in astrocytes slowed IRIF resolution it did not in hESCs, suggesting that repair in hESCs does not utilize DNA-PKcs. Altogether, our results show that hESCs have efficient DSB repair that is largely ATR-dependent HRR, whereas astrocytes critically depend on ATM for NHEJ, which, in part, is DNA-PKcs-independent.

  3. PRP19 transforms into a sensor of RPA-ssDNA after DNA damage and drives ATR activation via a ubiquitin-mediated circuitry.

    Science.gov (United States)

    Maréchal, Alexandre; Li, Ju-Mei; Ji, Xiao Ye; Wu, Ching-Shyi; Yazinski, Stephanie A; Nguyen, Hai Dang; Liu, Shizhou; Jiménez, Amanda E; Jin, Jianping; Zou, Lee

    2014-01-23

    PRP19 is a ubiquitin ligase involved in pre-mRNA splicing and the DNA damage response (DDR). Although the role for PRP19 in splicing is well characterized, its role in the DDR remains elusive. Through a proteomic screen for proteins that interact with RPA-coated single-stranded DNA (RPA-ssDNA), we identified PRP19 as a sensor of DNA damage. PRP19 directly binds RPA and localizes to DNA damage sites via RPA, promoting RPA ubiquitylation in a DNA-damage-induced manner. PRP19 facilitates the accumulation of ATRIP, the regulatory partner of the ataxia telangiectasia mutated and Rad3-related (ATR) kinase, at DNA damage sites. Depletion of PRP19 compromised the phosphorylation of ATR substrates, recovery of stalled replication forks, and progression of replication forks on damaged DNA. Importantly, PRP19 mutants that cannot bind RPA or function as an E3 ligase failed to support the ATR response, revealing that PRP19 drives ATR activation by acting as an RPA-ssDNA-sensing ubiquitin ligase during the DDR. Copyright © 2014 Elsevier Inc. All rights reserved.

  4. Investigation of ATR-FTIR spectroscopy as an alternative to the Water Leach Free Acidity test for cellulose acetate-based film

    DEFF Research Database (Denmark)

    Johansen, Karin Bonde; Shashoua, Yvonne

    2005-01-01

    Cellulose acetate film loses acetate groups on ageing which results in the formation of damaging acetic acid. Water-Leach Free Acidity Test (WLFAT) is the definitive technique to quantify acidity, but requires 1g film and 26 hours. ATR-FTIR spectroscopy is a non-destructive, rapid technique which...

  5. Bacterial and abiotic decay in waterlogged archaeological Picea abies (L.) Karst studied by confocal Raman imaging and ATR-FTIR spectroscopy

    DEFF Research Database (Denmark)

    Pedersen, Nanna Bjerregaard; Gierlinger, Notburga; Thygesen, Lisbeth Garbrecht

    2015-01-01

    Waterlogged archaeological Norway spruce [Picea abies (L.) Karst] poles were studied by means of confocal Raman imaging (CRI) and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) analysis to determine lignin and polysaccharide composition and distribution in the cell......, and minor oxidation of the lignin polymer compared to recent reference material. This is evidence for abiotic decay in the course of waterlogging....

  6. Revisiting the Brønsted acid catalysed hydrolysis kinetics of polymeric carbohydrates in ionic liquids by in situ ATR-FTIR spectroscopy

    DEFF Research Database (Denmark)

    Kunov-Kruse, Andreas Jonas; Riisager, Anders; Shunmugavel, Saravanamurugan

    2013-01-01

    A new versatile method to measure rates and determine activation energies for the Brønsted acid catalysed hydrolysis of cellulose and cellobiose (and other polymeric carbohydrates) in ionic liquids is demonstrated by following the C–O stretching band of the glycoside bond with in situ ATR...

  7. Measuring Heterogeneous Reaction Rates with ATR-FTIR Spectroscopy to Evaluate Chemical Fates in an Atmospheric Environment: A Physical Chemistry and Environmental Chemistry Laboratory Experiment

    Science.gov (United States)

    Roberts, Jason E.; Zeng, Guang; Maron, Marta K.; Mach, Mindy; Dwebi, Iman; Liu, Yong

    2016-01-01

    This paper reports an undergraduate laboratory experiment to measure heterogeneous liquid/gas reaction kinetics (ozone-oleic acid and ozone-phenothrin) using a flow reactor coupled to an attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectrometer. The experiment is specially designed for an upper-level undergraduate Physical…

  8. Rapid, nondestructive estimation of surface polymer layer thickness using attenuated total reflection fourier transform infrared (ATR FT-IR) spectroscopy and synthetic spectra derived from optical principles.

    Science.gov (United States)

    Weinstock, B André; Guiney, Linda M; Loose, Christopher

    2012-11-01

    We have developed a rapid, nondestructive analytical method that estimates the thickness of a surface polymer layer with high precision but unknown accuracy using a single attenuated total reflection Fourier transform infrared (ATR FT-IR) measurement. Because the method is rapid, nondestructive, and requires no sample preparation, it is ideal as a process analytical technique. Prior to implementation, the ATR FT-IR spectrum of the substrate layer pure component and the ATR FT-IR and real refractive index spectra of the surface layer pure component must be known. From these three input spectra a synthetic mid-infrared spectral matrix of surface layers 0 nm to 10,000 nm thick on substrate is created de novo. A minimum statistical distance match between a process sample's ATR FT-IR spectrum and the synthetic spectral matrix provides the thickness of that sample. We show that this method can be used to successfully estimate the thickness of polysulfobetaine surface modification, a hydrated polymeric surface layer covalently bonded onto a polyetherurethane substrate. A database of 1850 sample spectra was examined. Spectrochemical matrix-effect unknowns, such as the nonuniform and molecularly novel polysulfobetaine-polyetherurethane interface, were found to be minimal. A partial least squares regression analysis of the database spectra versus their thicknesses as calculated by the method described yielded an estimate of precision of ±52 nm.

  9. Roles of nibrin and ATM/ATR kinases on the G2 checkpoint under endogenous or radio-induced DNA damage

    Directory of Open Access Journals (Sweden)

    Katherine Marcelain

    2005-01-01

    Full Text Available Checkpoint response to DNA damage involves the activation of DNA repair and G2 lengthening subpathways. The roles of nibrin (NBS1 and the ATM/ATR kinases in the G2 DNA damage checkpoint, evoked by endogenous and radio-induced DNA damage, were analyzed in control, A-T and NBS lymphoblast cell lines. Short-term responses to G2 treatments were evaluated by recording changes in the yield of chromosomal aberrations in the ensuing mitosis, due to G2 checkpoint adaptation, and also in the duration of G2 itself. The role of ATM/ATR in the G2 checkpoint pathway repairing chromosomal aberrations was unveiled by caffeine inhibition of both kinases in G2. In the control cell lines, nibrin and ATM cooperated to provide optimum G2 repair for endogenous DNA damage. In the A-T cells, ATR kinase substituted successfully for ATM, even though no G2 lengthening occurred. X-ray irradiation (0.4 Gy in G2 increased chromosomal aberrations and lengthened G2, in both mutant and control cells. However, the repair of radio-induced DNA damage took place only in the controls. It was associated with nibrin-ATM interaction, and ATR did not substitute for ATM. The absence of nibrin prevented the repair of both endogenous and radio-induced DNA damage in the NBS cells and partially affected the induction of G2 lengthening.

  10. Penentuan Prioritas Pengembangan Infrastruktur Kawasan Wisata Bahari Di Desa Sumberejo, Desa Lojejer, Dan Desa Puger Kulon, Kabupaten Jember Berdasarkan Preferensi Pengunjung Dan Masyarakat

    Directory of Open Access Journals (Sweden)

    Rr. Ajeng Gita Astami

    2015-03-01

    Full Text Available Kabupaten Jember memiliki kawasan wisata bahari dengan lima pantai yang berkarakteristik berbeda-beda pada ODTW yang dimiliki. Pantai Watu Ulo, Pantai Papuma, dan Pantai Puger memiliki jumlah pengunjung yang mengalami peningkatan setiap tahunnya dan merupakan penyumbang kontribusi terbesar pada jumlah wisatawan di Kabupaten Jember. Akan tetapi hal tersebut tidak diimbangi dengan adanya infrastruktur kawasan wisata yang memadai untuk mendukung kegiatan wisata di dalamnya. Penelitian ini dilakukan untuk menganalisa prioritas pengembangan infrastruktur berdasarkan preferensi pengunjung dan masyarakat melalui tingkat kepentingan dan kinerja infrastruktur kawasan wisata bahari dengan teknik IPA (Importance-Performance Analysis. Hasil dari penelitian ini adalah berupa prioritas pengembangan infrastruktur dalam mendukung kegiatan wisata bahari di Kabupaten Jember. Infrastruktur yang menjadi prioritas pengembangan di kawasan wisata bahari Kabupaten Jember adalah kondisi jalan, moda transportasi, fasilitas pendukung transportasi, sarana rekreasi, dan fasilitas pelayanan kesehatan.

  11. Evaluation of Corrosion of the Dummy ''EE'' Plate 19 in YA Type ATR Fuel Element During Reactor PALM Cycles

    International Nuclear Information System (INIS)

    Brower, Jeffrey Owen; Glazoff, Michael Vasily; Eiden, Thomas John; Rezvoi, Aleksey Victor

    2016-01-01

    Advanced Test Reactor (ATR) Cycle 153B-1 was a 14-day, high-power, powered axial locator mechanism (PALM) operating cycle that completed on April 12, 2013. Cycle 153B-1 was a typical operating cycle for the ATR and did not result in any unusual plant transients. ATR was started up and shut down as scheduled. The PALM drive physically moves the selected experiments into and out of the core to simulate reactor startup and heat up, and shutdown and cooldown transients, while the reactor remains in steady state conditions. However, after the cycle was over, several thousand of the flow-assisted corrosion pits and ''horseshoeing'' defects were readily observable on the surface of the several YA-type fuel elements (these are ''dummy'' plates that contain no fuel). In order understand these corrosion phenomena a thermal-hydraulic model of coolant channel 20 on a YA-M fuel element was generated. The boundaries of the model were the aluminum EE plate of a YA-M fuel element and a beryllium reflector block with 13 horizontal saw cuts which represented regions of zero flow. The heat generated in fuel plates 1 through 18 was modeled to be passing through the aluminum EE plate. The coolant channel 20 width was set at 0.058 in. (58 mils). It was established that the horizontal saw cuts had a significant effect on the temperature of the coolant. The flow, which was expected to vary linearly with gradual heating of the coolant as it passed through the channel, was extremely turbulent. The temperature rise, which was expected to be a smooth ''S'' curve, was represented by a series temperature rise ''humps,'' which occurred at each horizontal saw cut in the beryllium reflector block. Each of the 13 saw cuts had a chamfered edge which resulted in the coolant flow being re-directed as a jet across the coolant channel into the surface of the EE plate, which explained the temperature rise and the observed sscalloping and possibly pitting degradation on the YA-M fuel elements. In the case

  12. A distrofia miotônica ou miotonia atrófica: estudo sintético-crítico

    Directory of Open Access Journals (Sweden)

    Enzo Azzi

    1953-03-01

    Full Text Available O autor, baseado em algumas observações clínicas pessoais, individualiza os elementos principais (atróficos, miotônicos, neurendócrino-vegetativos que se referem à distrofia miotônica, insistindo sôbre a riqueza dos sintomas infundíbulo-hipofisários observados (distrofia adiposogenital, policitemia, perturbações do sono, constante hipoplasia da sela turca que falam em favor de uma meiopragia diencéfalo-hipofisária como mecanismo fisiopatogênico da doença. O autor discute também o problema das relações entre distrofia miotônica e miotonia de Thomsen, concluindo, com base em um de seus casos, pela identidade fundamental das duas formas e, portanto, pela sua unificação.

  13. PLS models for determination of SARA analysis of Colombian vacuum residues and molecular distillation fractions using MIR-ATR

    Directory of Open Access Journals (Sweden)

    Jorge A. Orrego-Ruiz

    2014-06-01

    Full Text Available In this work, prediction models of Saturates, Aromatics, Resins and Asphaltenes fractions (SARA from thirty-seven vacuum residues of representative Colombian crudes and eighteen fractions of molecular distillation process were obtained. Mid-Infrared (MIR Attenuated Total Reflection (ATR spectroscopy in combination with partial least squares (PLS regression analysis was used to estimate accurately SARA analysis in these kind of samples. Calibration coefficients of prediction models were for saturates, aromatics, resins and asphaltenes fractions, 0.99, 0.96, 0.97 and 0.99, respectively. This methodology permits to control the molecular distillation process since small differences in chemical composition can be detected. Total time elapsed to give the SARA analysis per sample is 10 minutes.

  14. Blood glucose measurement with multiple quantum cascade lasers using hollow-optical fiber-based ATR spectroscopy

    Science.gov (United States)

    Yoshioka, K.; Kino, S.; Matsuura, Y.

    2018-02-01

    For non-invasive blood glucose measurement, a measurement system based on mid-infrared ATR spectroscopy equipped with a combination of a QCL as a light source and a hollow-optical fiber as a beam delivery medium is developed. Firstly the measurement sensitivity of the system is evaluated by using glucose solutions and the result shows a significant correlation between optical absorbance and solution concentration. It is also confirmed that the system has a sensitivity that is enough for blood glucose measurement. Then optical absorption of human lips in the mid-infrared wavelength region is measured using a QCL with a wavenumber of 1080 cm-1 where human tissue exhibits strong absorption of glucose and its metabolites. As a result, the measured absorption follows the change of blood glucose well with a time delay of around 10 minutes and correlation factor between the absorbance and the blood glucose level is 0.42.

  15. A Simple Approach to Distinguish Classic and Formaldehyde-Free Tannin Based Rigid Foams by ATR FT-IR

    Directory of Open Access Journals (Sweden)

    Gianluca Tondi

    2015-01-01

    Full Text Available Tannin based rigid foams (TBRFs have been produced with formaldehyde since 1994. Only recently several methods have been developed in order to produce these foams without using formaldehyde. TBRFs with and without formaldehyde are visually indistinguishable; therefore a method for determining the differences between these foams had to be found. The attenuated total reflectance infrared spectroscopy (ATR FT-IR investigation of the TBRFs presented in this paper allowed discrimination between the formaldehyde-containing (classic and formaldehyde-free TBRFs. The spectra of the formaldehyde-free TBRFs, indeed, present decreased band intensity related to the C–O stretching vibration of (i the methylol groups and (ii the furanic rings. This evidence served to prove the chemical difference between the two TBRFs and explained the slightly higher mechanical properties measured for the classic TBRFs.

  16. Fast and calibration free determination of first order reaction kinetics in API synthesis using in-situ ATR-FTIR.

    Science.gov (United States)

    Rehbein, Moritz C; Husmann, Sascha; Lechner, Christian; Kunick, Conrad; Scholl, Stephan

    2018-05-01

    In early stages of drug development only sparse amounts of the key substances are available, which is problematic for the determination of important process data like reaction kinetics. Therefore, it is important to perform experiments as economically as possible, especially in regards to limiting compounds. Here we demonstrate the use of a temperature step experiment enabling the determination of complete reaction kinetics in a single non-isothermal experiment. In contrast to the traditionally used HPLC, the method takes advantage of the high measuring rate and the low amount of labor involved in using in-situ ATR-FTIR to determine time-dependent concentration-equivalent data. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. Formulation and drug-content assay of microencapsulated antisense oligonucleotide to NF-κB using ATR-FTIR

    International Nuclear Information System (INIS)

    Siwale, Rodney; Meadows, Fred; Mody, Vicky V; Shah, Samit

    2013-01-01

    Antisense oligonucleotide to NF-κB sequence: 5′-GGA AAC ACA TCC TCC ATG-3′, was microencapsulated in an albumin matrix by the method of spray drying TM . Spectral analysis was performed on varying drug loading formulations of both drugs by mid-IR attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). An out of plane O–H bending vibration at 948 cm −1 , unique to both the native and microencapsulated drugs was identified. The calculated peak areas corresponded to the drug loadings in the microsphere formulations. A standard curve could then be used to determine the drug content of an unknown microsphere formulation. Accuracy and precision were determined to be comparable to other analytical techniques such as HPLC. (paper)

  18. The Conference Proceedings of the 2001 Air Transport Research Society (ATRS) of the WCTR Society. Volume 1

    Science.gov (United States)

    Lee, Yeong-Heok (Editor); Bowen, Brent D. (Editor); Tarry, Scott E. (Editor)

    2001-01-01

    The ATRS held its Annual conference at Jeju Island, Korea in July 2001. The conference was a success with nearly 140 participants including 70 presenters. This report contains presentations from Volume 1 on the following: Airline and Travel Agent Relationships in Asia;Benchmarking Aviation Safety in the Commercial Airline Industry;Impact of Frequent Flyer Program on the Demand for Air Travel; Application of Genetic Algorithm on Airline Schedule;The Effects of Dual Carrier Designation and Partial Liberalization: The Case of Canada;Defense of Air Carriers and Air Agencies in FAA Enforcement proceedin gs - Damage Control Before the Case Arises; Cost Incentives for Airline Mergers? - An examination on the cost impact of U.S. airline mergers and acquisitions;Airport Regulation, Airline Competition and Canada's Airport System; Airline Competition: The Case of Israel's Domestic Doupoly; Non-Financial Indicators of Airline Distress: A Conceptual Approach;and Airport Privatization: An Empirical Analysis of Financial and Operational Efficiency.

  19. Inhibition of DNA-PKcs enhances radiosensitivity and increases the levels of ATM and ATR in NSCLC cells exposed to carbon ion irradiation.

    Science.gov (United States)

    Yang, Lina; Liu, Yuanyuan; Sun, Chao; Yang, Xinrui; Yang, Zhen; Ran, Juntao; Zhang, Qiuning; Zhang, Hong; Wang, Xinyu; Wang, Xiaohu

    2015-11-01

    Non-small cell lung cancer (NSCLC) exhibits radioresistance to conventional rays, due to its DNA damage repair systems. NSCLC may potentially be sensitized to radiation treatment by reducing those factors that continuously enhance the repair of damaged DNA. In the present study, normal lung fibroblast MRC-5 and lung cancer A549 cells were treated with NU7026 and CGK733, which are inhibitors of the DNA-dependent protein kinase catalytic subunit (PKcs) and ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR), respectively, followed by exposure to X-rays and carbon ion irradiation. The cytotoxic activity, cell survival rate, DNA damage repair ability, cell cycle arrest and apoptosis rate of the treated cells were analyzed with MTT assay, colony formation assay, immunofluorescence and flow cytometry, respectively. The transcription and translation levels of the ATM, ATR and DNA-PKcs genes were detected by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. The results indicated that the radiosensitivity and DNA repair ability of A549 cells were reduced, and the percentages of apoptotic cells and those arrested at the G 2 /M phase of the cell cycle were significantly increased, following ionizing radiation with inhibitor-pretreatment. The expression levels of ATM, ATR, DNA-PKcs and phosphorylated histone H2AX, a biomarker for DNA double-strand breaks, were all upregulated at the transcriptional or translational level in A549 cells treated with carbon ion irradiation, compared with the control and X-rays-treated cells. In addition, the treatment with 5-50 µM NU7026 or CGK733 did not produce any obvious cytotoxicity in MRC-5 cells, and the effect of the DNA-PKcs-inhibitor on enhancing the radiosensitivity of A549 cells was stronger than that observed for the ATM and ATR-inhibitor. These findings demonstrated a minor role for ATM and ATR in radiation-induced cell death, since the upregulation of

  20. The Neurospora crassa UVS-3 epistasis group encodes homologues of the ATR/ATRIP checkpoint control system.

    Science.gov (United States)

    Kazama, Yusuke; Ishii, Chizu; Schroeder, Alice L; Shimada, Hisao; Wakabayashi, Michiyoshi; Inoue, Hirokazu

    2008-02-01

    The mutagen sensitive uvs-3 and mus-9 mutants of Neurospora show mutagen and hydroxyurea sensitivity, mutator effects and duplication instability typical of recombination repair and DNA damage checkpoint defective mutants. To determine the nature of these genes we used cosmids from a genomic library to clone the uvs-3 gene by complementation for MMS sensitivity. Mutation induction by transposon insertion and RIP defined the coding sequence. RFLP analysis confirmed that this sequence maps in the area of uvs-3 at the left telomere of LG IV. Analysis of the cDNA showed that the UVS-3 protein contains an ORF of 969 amino acids with one intron. It is homologous to UvsD of Aspergillus nidulans, a member of the ATRIP family of checkpoint proteins. It retains the N' terminal coiled-coil motif followed by four basic amino acids typical of these proteins and shows the highest homology in this region. The uvsD cDNA partially complements the defects of the uvs-3 mutation. The uvs-3 mutant shows a higher level of micronuclei in conidia and failure to halt germination and nuclear division in the presence of hydroxyurea than wild type, suggesting checkpoint defects. ATRIP proteins bind tightly to ATR PI-3 kinase (phosphatidylinositol 3-kinase) proteins. Therefore, we searched the Neurospora genome sequence for homologues of the Aspergillus nidulans ATR, UvsB. A uvsB homologous sequence was present in the right arm of chromosome I where the mus-9 gene maps. A cosmid containing this genomic DNA complemented the mus-9 mutation. The putative MUS-9 protein is 2484 amino acids long with eight introns. Homology is especially high in the C-terminal 350 amino acids that correspond to the PI-3 kinase domain. In wild type a low level of constitutive mRNA is present for both genes. It is transiently induced upon UV exposure.

  1. Overview of aerosol properties associated with air masses sampled by the ATR-42 during the EUCAARI campaign (2008

    Directory of Open Access Journals (Sweden)

    S. Crumeyrolle

    2013-05-01

    Full Text Available Within the frame of the European Aerosol Cloud Climate and Air Quality Interactions (EUCAARI project, the Météo-France aircraft ATR-42 performed 22 research flights over central Europe and the North Sea during the intensive observation period in May 2008. For the campaign, the ATR-42 was equipped to study the aerosol physical, chemical, hygroscopic and optical properties, as well as cloud microphysics. For the 22 research flights, retroplume analyses along the flight tracks were performed with FLEXPART in order to classify air masses into five sectors of origin, allowing for a qualitative evaluation of emission influence on the respective air parcel. This study shows that the extensive aerosol parameters (aerosol mass and number concentrations show vertical decreasing gradients and in some air masses maximum mass concentrations (mainly organics in an intermediate layer (1–3 km. The observed mass concentrations (in the boundary layer (BL: between 10 and 30 μg m−3; lower free troposphere (LFT: 0.8 and 14 μg m−3 are high especially in comparison with the 2015 European norms for PM2.5 (25 μg m−3 and with previous airborne studies performed over England (Morgan et al., 2009; McMeeking et al., 2012. Particle number size distributions show a larger fraction of particles in the accumulation size range in the LFT compared to BL. The chemical composition of submicron aerosol particles is dominated by organics in the BL, while ammonium sulphate dominates the submicron aerosols in the LFT, especially in the aerosol particles originated from north-eastern Europe (~ 80%, also experiencing nucleation events along the transport. As a consequence, first the particle CCN acting ability, shown by the CCN/CN ratio, and second the average values of the scattering cross sections of optically active particles (i.e. scattering coefficient divided by the optical active particle concentration are increased in the LFT compared to BL.

  2. In situ ATR FTIR studies of SO4 adsorption on goethite in the presence of copper ions.

    Science.gov (United States)

    Beattie, D A; Chapelet, J K; Gräfe, M; Skinner, W M; Smith, E

    2008-12-15

    Despite the existence of many single ion sorption studies on iron and aluminum oxides, fewer studies have been reported that describe cosorption reactions. In this work, we present an in situ ATR FTIR study of synergistic adsorption of sulfate (SO4) and copper (Cu) on goethite, which is representative of the minerals and ions present in mine wastes, acid sulfate soils, and other industrial and agricultural settings. Sulfate adsorption was studied as a function of varying pH, and as a function of increasing concentration in the absence and presence of Cu. The presence of Cu ions in solution had a complex effect on the ability of SO4 ions to be retained on the goethite surface with increasing pH, with complete desorption occurring near pH 7 and 9 in the absence and presence of Cu, respectively. In addition, Cu ions altered the balance of inner vs outer sphere adsorbed SO4. The solid phase partitioning of SO4 at pH 3 and pH 5 was elevated by the presence of Cu; in both cases Cu increased the affinity of SO4 for the goethite surface. Complementary ex situ sorption edge studies of Cu on goethite in the absence and presence of SO4 revealed that the Cu adsorption edge shifted to lower pH (6.3 --> 5.6) in the presence of SO4, consistent with a decrease of the electrostatic repulsion between the goethite surface and adsorbing Cu. Based on the ATR FTIR and bulk sorption data we surmise that the cosorption products of SO4 and Cu at the goethite-water interface were not in the nature of ternary complexes under the conditions studied here. This information is critical for the evaluation of the onset of surface precipitates of copper-hydroxy sulfates as a function of pH and solution concentration.

  3. ATR-FTIR Spectroscopic Evidence for Biomolecular Phosphorus and Carboxyl Groups Facilitating Bacterial Adhesion to Iron Oxides

    Science.gov (United States)

    Parikh, Sanjai J.; Mukome, Fungai N.D.; Zhang, Xiaoming

    2014-01-01

    Attenuated total reflectance (ATR) Fourier transform infrared (FTIR) spectroscopy has been used to probe the binding of bacteria to hematite (α-Fe2O3) and goethite (α-FeOOH). In situ ATR-FTIR experiments with bacteria (Pseudomonas putida, P. aeruginosa, Escherichia coli), mixed amino acids, polypeptide extracts, deoxyribonucleic acid (DNA), and a suite of model compounds were conducted. These compounds represent carboxyl, catecholate, amide, and phosphate groups present in siderophores, amino acids, polysaccharides, phospholipids, and DNA. Due in part to the ubiquitous presence of carboxyl groups in biomolecules, numerous IR peaks corresponding to outer-sphere or unbound (1400 cm−1) and inner-sphere (1310-1320 cm−1) coordinated carboxyl groups are noted following reaction of bacteria and biomolecules with α-Fe2O3 and α-FeOOH. However, the data also reveal that the presence of low-level amounts (i.e., 0.45-0.79%) of biomolecular phosphorous groups result in strong IR bands at ~1043 cm−1, corresponding to inner-sphere Fe-O-P bonds, underscoring the importance of bacteria associated P-containing groups in biomolecule and cell adhesion. Spectral comparisons also reveal slightly greater P-O-Fe contributions for bacteria (Pseudomonad, E. coli) deposited on α-FeOOH, as compared to α-Fe2O3. This data demonstrates that slight differences in bacterial adhesion to Fe oxides can be attributed to bacterial species and Fe-oxide minerals. However, more importantly, the strong binding affinity of phosphate in all bacteria samples to both Fe-oxides results in the formation of inner-sphere Fe-O-P bonds, signifying the critical role of biomolecular P in the initiation of bacterial adhesion. PMID:24859052

  4. ANALISIS TATA KELOLA OPTIMALISASI SUMBER DAYA SISTEM INFORMASI MANAJEMEN JEMBATAN TIMBANG (EDM04 BERDASARKAN KERANGKA KERJA COBIT 5 PADA DINAS PERHUBUNGAN KOMUNIKASI DAN INFORMATIKA PROVINSI JAWA TENGAH

    Directory of Open Access Journals (Sweden)

    Yudistira Dian Hastiti

    2016-06-01

    Full Text Available Dinas Perhubungan Komunikasi dan Informatika (Dinhubkominfo Provinsi Jawa tengah sebagai perumus dan pelaksana kebijakan teknis, fasilitator, dan evaluator terkait penyelengaraan kegiatan penimbangan kendaraan bermotor melalui 16 jembatan timbang yang tersebar di wilayah provinsi jawa tengah telah mengimplementasikan sebuah system yang dipergunakan untuk kemudahan dalam menimbang kendaraan dengan mendata setiap kendaraan pada suatu system yang disebut Sistem Informasi Manajemen Jembatan Timbang (SIM JT. Masalah yang ditemukan saat ini yaitu, melihat padatnya antrian panjang kendaraan yang akan ditimbang disebabkan karena SIM JT yang belum memiliki database identitas kendaraan dan tidak dapat terpantau secara real time yang terjadi saat local server dalam keadaan down. Berdasarkan hal tersebut Dinhubkominfo berupaya mengoptimalkan kinerja SIM JT baik dari segi sumber daya manajemen manusia, TI, serta keuangan untuk meminimalkan kesalahan serta meningkatkan efektifitas pelayanan. Dari hasil studi dokumen, wawancara, dan kuesioner  berdasarkan COBIT 5 menghasilkan tingkat kapabilitas tata kelola proses optimalisasi sumer daya (EDM04 pada Dinas Perhubungan Komunikasi dan Informatika Provinsi Jawa Tengah saat ini berada di level 3 dengan status pencapaian Largely Achieved sebesar 80,18% setara dengan 3,80 dimana level 0, 1, dan 2 mencapai status Fully Achieved. Hal ini menunjukan telah mengelola dengan baik proses optimalisasi sumber daya dan diimplementasikan untuk mendukung pengerjaan proses standar dan efektif. Untuk mencapai tingkat target, Dinhubkominfo dapat melakukan strategi perbaikan dengan memperhatikan secara bertahap dari proses atribut level 1 sampai 4 Kata Kunci: Analisis Tata Kelola TI, COBIT 5, Sistem Informasi Manajemen Jembatan Timbang, Analisis Tingkat Kapabilitas, Analisis Kesenjangan.

  5. The orally active and bioavailable ATR kinase inhibitor AZD6738 potentiates the anti-tumor effects of cisplatin to resolve ATM-deficient non-small cell lung cancer in vivo.

    Science.gov (United States)

    Vendetti, Frank P; Lau, Alan; Schamus, Sandra; Conrads, Thomas P; O'Connor, Mark J; Bakkenist, Christopher J

    2015-12-29

    ATR and ATM are DNA damage signaling kinases that phosphorylate several thousand substrates. ATR kinase activity is increased at damaged replication forks and resected DNA double-strand breaks (DSBs). ATM kinase activity is increased at DSBs. ATM has been widely studied since ataxia telangiectasia individuals who express no ATM protein are the most radiosensitive patients identified. Since ATM is not an essential protein, it is widely believed that ATM kinase inhibitors will be well-tolerated in the clinic. ATR has been widely studied, but advances have been complicated by the finding that ATR is an essential protein and it is widely believed that ATR kinase inhibitors will be toxic in the clinic. We describe AZD6738, an orally active and bioavailable ATR kinase inhibitor. AZD6738 induces cell death and senescence in non-small cell lung cancer (NSCLC) cell lines. AZD6738 potentiates the cytotoxicity of cisplatin and gemcitabine in NSCLC cell lines with intact ATM kinase signaling, and potently synergizes with cisplatin in ATM-deficient NSCLC cells. In contrast to expectations, daily administration of AZD6738 and ATR kinase inhibition for 14 consecutive days is tolerated in mice and enhances the therapeutic efficacy of cisplatin in xenograft models. Remarkably, the combination of cisplatin and AZD6738 resolves ATM-deficient lung cancer xenografts.

  6. Evaluation of Erosion of the Dummy ''EE'' Plate 19 in YA Type ATR Fuel Element During Reactor PALM Cycles

    International Nuclear Information System (INIS)

    Brower, Jeffrey O.; Glazoff, Michael V.; Eiden, Thomas J.; Rezvoi, Aleksey V.

    2016-01-01

    Advanced Test Reactor (ATR) Cycle 153B-1 was a 14-day, high-power, powered axial locator mechanism (PALM) operating cycle that completed on April 12, 2013. Cycle 153B-1 was a typical operating cycle for the ATR, and did not result in any unusual plant transients. ATR was started up and shut down as scheduled. The PALM drive physically moves the selected experiments into and out of the core to simulate reactor startup and heat up, and shutdown and cooldown transients, while the reactor remains in steady-state conditions. However, after the cycle was over, when the fuel elements were removed from the core and inspected, several thousand flow-assisted erosion pits and ''horseshoeing'' defects were readily observed on the surface of the several YA-type fuel elements (these are aluminum ''dummy'' plates that contain no fuel). In order to understand these erosion phenomena, a thermal-hydraulic model of coolant channel 20 on a YA-M fuel element was generated. The boundaries of the model were the aluminum EE plate of a YA-M fuel element and a beryllium reflector block with 13 horizontal saw cuts which represented regions of zero flow. The heat generated in fuel plates 1 through 18 was modeled to be passing through the aluminum EE plate. The coolant channel 20 width was set at 0.058 in. (58 mils). It was established that the horizontal saw cuts had a significant effect on the temperature of the coolant. The flow, which was expected to vary linearly with gradual heating of the coolant as it passed through the channel, was extremely turbulent. The temperature rise, which was expected to be a smooth ''S'' curve, was represented by a series temperature rise ''humps,'' which occurred at each horizontal saw cut in the beryllium reflector block. Each of the 13 saw cuts had a chamfered edge which resulted in the coolant flow being re-directed as a jet across the coolant channel into the surface of the EE plate, which explained the temperature rise and the observed scalloping and

  7. Hubungan Kadar Iodium dalam Garam Beriodium di Rumah Tangga dengan Kecukupan Iodium Berdasarkan Nilai Ekskresi Iodium Urin (EIU pada Wanita Usia Subur

    Directory of Open Access Journals (Sweden)

    Donny Kristanto Mulyantoro

    2015-01-01

    tanah dan air setempat kurang mengandung iodium. Pada daerah tersebut Wanita Usia Subur (WUS adalah segmen penduduk yang rawan mengalami GAKI. Sebagai upaya penanggulangan, saat ini dilakukan fortifikasi iodium pada garam. Tujuan penelitian ini untuk mengukur hubungan antara kadar iodium dalam garam beriodium di rumah tangga dengan kecukupan iodium berdasarkan nilai ekskresi iodium urin (EIU. Penelitian ini merupakan penelitian observasional dengan desain potong lintang. Lokasi penelitian di Desa Kragilan, Kecamatan Pakis, Kabupaten Magelang. Sebanyak 68 wanita usia 18 – 45 tahun terlibat dalam penelitian ini, yang diukur kadar iodium dalam garam yang digunakan di rumah tangga, kecukupan iodium berdasarkan kadar iodium urin (EIU, konsumsi garam beriodium dan makanan sumber iodium. Hasil penelitian menunjukkan bahwa median EIU sebesar 124,6 μg/L, proporsi subyek penelitian mengalami defisiensi iodium sebesar 41,1%, dan mengalami excess sebesar 5,9%. Rata-rata kadar iodium dalam garam beriodium di rumah tangga sebesar 19,6 ppm dan proporsi garam beriodium memenuhi syarat (≥ 30 ppm KIO3 sebesar 52,9%. Hasil analisis korelasi pearson menunjukkan ada hubungan bermakna antara kadar iodium dalam garam beriodium di rumah tangga dengan kecukupan iodium berdasarkan nilai EIU (rho = 0,5, p < 0,01. Hasil analisis multi variabel diperoleh persamaan Y = 22,199 + 6,076 X1. Dengan persamaan tersebut, untuk memenuhi kebutuhan iodium, kadar iodium dalam garam beriodium berkisar antara 13 – 29 ppm iodium atau 22 – 49 ppm KIO3. Kata kunci: iodium, garam beriodium, kecukupan iodium, EIU, wanita.

  8. PENERAPAN K-OPTIMAL PADA ALGORITMA KNN UNTUK PREDIKSI KELULUSAN TEPAT WAKTU MAHASISWA PROGRAM STUDI ILMU KOMPUTER FMIPA UNLAM BERDASARKAN IP SAMPAI DENGAN SEMESTER 4

    Directory of Open Access Journals (Sweden)

    Mutiara Ayu Banjarsari

    2016-04-01

    Full Text Available The data pile on a database of academic information systems at Computer Science Program of Mathematic and Natural Science Faculty of Lambung Mangkurat University is not fully utilized, although it can provide new information that has not been known before. Data mining techniques can be used to predict the timely graduation of students. The k-Nearest Nieghbor, a method to classify objects based on training data located closest to the object, was used in this study. Selection of the value of k in kNN algorithm became important because it would affect the performance of the algorithm kNN, therefore it was necessary to know how the value of k and the level of accuracy. The k-Fold Cross Validation method and Accuracy Test was used to determine the value of k-Optimal. The result showed that the value of k = 5 was defined as k-Optimal which was then be applied in the kNN algorithm for prediction of timely graduation of students based on the Grade Point Average up to 4th semester. Keywords: kNN, k-Optimal, Classification, Data mining, k-Fold Cross Validation method Tumpukan data pada database sistem informasi akademik Program Studi Ilmu Komputer FMIPA Unlam belum dimanfaatkan secara maksimal, padahal dari data tersebut dapat memberikan sebuah informasi baru yang belum diketahui sebelumnya. Teknik data mining dapat digunakan untuk memprediksi kelulusan tepat waktu mahasiswa. Penelitian menggunakan metode k-Nearest Nieghbor yang merupakan sebuah metode untuk melakukan klasifikasi terhadap objek berdasarkan data training yang jaraknya paling dekat dengan objek tersebut. Pemilihan nilai k pada algoritma kNN menjadi hal yang penting karena akan mempengaruhi kinerja dari algoritma kNN, oleh karena itu perlu diketahui berapa nilai k dan tingkat akurasinya. Metode k-Fold Cross Validation dan Uji Akurasi digunakan untuk mengetahui nilai k-Optimal. Hasil yang didapat adalah nilai k=5 dengan tingkat akurasi sebesar 80.00% yang ditetapkan sebagai k-Optimal. Nilai k

  9. Atròfia muscular espinal: mecanismes patogènics i estratègies terapèutiques en models murins de la malaltia

    OpenAIRE

    Cerveró Cebrià, Clàudia

    2016-01-01

    L’atròfia muscular espinal (AME) és una malaltia genètica que cursa amb mort de motoneurones espinals i atròfia muscular. S’ha caracteritzat un model murí d’AME, l’Smn2B/-, amb una clínica menys severa que la mostrada per altres models més extensament utilitzats. S’ha evidenciat una alteració multisistèmica acompanyant a la clàssicament coneguda del sistema neuromuscular. S’han estudiat les sinapsis colinèrgiques tipus C en l’AME i testat el paper del PRE-084 (agonista del receptor sigm...

  10. In-line ATR-UV and Raman Spectroscopy for Monitoring API Dissolution Process During Liquid-Filled Soft-Gelatin Capsule Manufacturing.

    Science.gov (United States)

    Wan, Boyong; Zordan, Christopher A; Lu, Xujin; McGeorge, Gary

    2016-10-01

    Complete dissolution of the active pharmaceutical ingredient (API) is critical in the manufacturing of liquid-filled soft-gelatin capsules (SGC). Attenuated total reflectance UV spectroscopy (ATR-UV) and Raman spectroscopy have been investigated for in-line monitoring of API dissolution during manufacturing of an SGC product. Calibration models have been developed with both techniques for in-line determination of API potency. Performance of both techniques was evaluated and compared. The ATR-UV methodology was found to be able to monitor the dissolution process and determine the endpoint, but was sensitive to temperature variations. The Raman technique was also capable of effectively monitoring the process and was more robust to the temperature variation and process perturbations by using an excipient peak for internal correction. Different data preprocessing methodologies were explored in an attempt to improve method performance.

  11. Formation of carboxy- and amide-terminated alkyl monolayers on silicon(111) investigated by ATR-FTIR, XPS, and X-ray scattering: Construction of photoswitchable surfaces

    DEFF Research Database (Denmark)

    Rück-Braun, Karola; Petersen, Michael Åxman; Michalik, Fabian

    2013-01-01

    -FTIR and XPS studies of the fulgimide samples revealed closely covered amide-terminated SAMs. Reversible photoswitching of the headgroup was read out by applying XPS, ATR-FTIR, and difference absorption spectra in the mid-IR. In XPS, we observed a reversible breathing of the amide/imide C1s and N1s signals......We have prepared high-quality, densely packed, self-assembled monolayers (SAMs) of carboxy-terminated alkyl chains on Si(111). The samples were made by thermal grafting of methyl undec-10-enoate under an inert atmosphere and subsequent cleavage of the ester functionality to disclose the carboxylic...... zigzag-like substitution pattern for the ester- and carboxy-terminated monolayer. Hydrolysis of the remaining H-Si(111) bonds at the surface furnished HO-Si(111) groups according to XPS and attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) studies. The amide-terminated alkyl...

  12. Pharmacologic ATM but not ATR kinase inhibition abrogates p21-dependent G1 arrest and promotes gastrointestinal syndrome after total body irradiation.

    Science.gov (United States)

    Vendetti, Frank P; Leibowitz, Brian J; Barnes, Jennifer; Schamus, Sandy; Kiesel, Brian F; Abberbock, Shira; Conrads, Thomas; Clump, David Andy; Cadogan, Elaine; O'Connor, Mark J; Yu, Jian; Beumer, Jan H; Bakkenist, Christopher J

    2017-02-01

    We show that ATM kinase inhibition using AZ31 prior to 9 or 9.25 Gy total body irradiation (TBI) reduced median time to moribund in mice to 8 days. ATR kinase inhibition using AZD6738 prior to TBI did not reduce median time to moribund. The striking finding associated with ATM inhibition prior to TBI was increased crypt loss within the intestine epithelium. ATM inhibition reduced upregulation of p21, an inhibitor of cyclin-dependent kinases, and blocked G1 arrest after TBI thereby increasing the number of S phase cells in crypts in wild-type but not Cdkn1a(p21 CIP/WAF1 )-/- mice. In contrast, ATR inhibition increased upregulation of p21 after TBI. Thus, ATM activity is essential for p21-dependent arrest while ATR inhibition may potentiate arrest in crypt cells after TBI. Nevertheless, ATM inhibition reduced median time to moribund in Cdkn1a(p21 CIP/WAF1 )-/- mice after TBI. ATM inhibition also increased cell death in crypts at 4 h in Cdkn1a(p21 CIP/WAF1 )-/-, earlier than at 24 h in wild-type mice after TBI. In contrast, ATR inhibition decreased cell death in crypts in Cdkn1a(p21 CIP/WAF1 )-/- mice at 4 h after TBI. We conclude that ATM activity is essential for p21-dependent and p21-independent mechanisms that radioprotect intestinal crypts and that ATM inhibition promotes GI syndrome after TBI.

  13. Parvovirus B19 NS1 protein induces cell cycle arrest at G2-phase by activating the ATR-CDC25C-CDK1 pathway.

    Directory of Open Access Journals (Sweden)

    Peng Xu

    2017-03-01

    Full Text Available Human parvovirus B19 (B19V infection of primary human erythroid progenitor cells (EPCs arrests infected cells at both late S-phase and G2-phase, which contain 4N DNA. B19V infection induces a DNA damage response (DDR that facilitates viral DNA replication but is dispensable for cell cycle arrest at G2-phase; however, a putative C-terminal transactivation domain (TAD2 within NS1 is responsible for G2-phase arrest. To fully understand the mechanism underlying B19V NS1-induced G2-phase arrest, we established two doxycycline-inducible B19V-permissive UT7/Epo-S1 cell lines that express NS1 or NS1mTAD2, and examined the function of the TAD2 domain during G2-phase arrest. The results confirm that the NS1 TAD2 domain plays a pivotal role in NS1-induced G2-phase arrest. Mechanistically, NS1 transactivated cellular gene expression through the TAD2 domain, which was itself responsible for ATR (ataxia-telangiectasia mutated and Rad3-related activation. Activated ATR phosphorylated CDC25C at serine 216, which in turn inactivated the cyclin B/CDK1 complex without affecting nuclear import of the complex. Importantly, we found that the ATR-CHK1-CDC25C-CDK1 pathway was activated during B19V infection of EPCs, and that ATR activation played an important role in B19V infection-induced G2-phase arrest.

  14. Evaluation of Salmon Adhesion on PET-Metal Interface by ATR, FT-IR, and Raman Spectroscopy

    Directory of Open Access Journals (Sweden)

    E. Zumelzu

    2015-01-01

    Full Text Available The material employed in this study is an ecoefficient, environmentally friendly, chromium (VI-free (noncarcinogenic metal polymer. The originality of the research lies in the study of the effect of new production procedures of salmon on metal packaging with multilayer polyethylene terephthalate (PET polymer coatings. Our hypothesis states that the adhesion of postmortem salmon muscles to the PET polymer coating produces surface and structural changes that affect the functionality and limit the useful life of metal containers, compromising therefore their recycling capacity as ecomaterials. This work is focused on studying the effects of the biochemical changes of postmortem salmon on the PET coating and how muscle degradation favors adhesion to the container. The experimental design considered a series of laboratory tests of containers simulating the conditions of canned salmon, chemical and physical tests of food-contact canning to evaluate the adhesion, and characterization of changes in the multilayer PET polymer by electron microscopy, ATR, FT-IR, and Raman spectroscopy analyses. The analyses determined the effect of heat treatment of containers on the loss of freshness of canned fish and the increased adhesion to the container wall, and the limited capability of the urea treatment to remove salmon muscle from the container for recycling purposes.

  15. AGR-2 Data Qualification Report for ATR Cycles 147A, 148A, 148B, and 149A

    Energy Technology Data Exchange (ETDEWEB)

    Michael L. Abbott; Keith A. Daum

    2011-08-01

    This report presents the data qualification status of fuel irradiation data from the first four reactor cycles (147A, 148A, 148B, and 149A) of the on-going second Advanced Gas Reactor (AGR-2) experiment as recorded in the NGNP Data Management and Analysis System (NDMAS). This includes data received by NDMAS from the period June 22, 2010 through May 21, 2011. AGR-2 is the second in a series of eight planned irradiation experiments for the AGR Fuel Development and Qualification Program, which supports development of the very high temperature gas-cooled reactor (VHTR) under the Next Generation Nuclear Plant (NGNP) Project. Irradiation of the AGR-2 test train is being performed at the Advanced Test Reactor (ATR) at the Idaho National Laboratory (INL) and is planned for 600 effective full power days (approximately 2.75 calendar years) (PLN-3798). The experiment is intended to demonstrate the performance of UCO (uranium oxycarbide) and UO2 (uranium dioxide) fuel produced in a large coater. Data qualification status of the AGR-1 experiment was reported in INL/EXT-10-17943 (Abbott et al. 2010).

  16. Neutron monitoring measurements for the CIT [Compact Ignition Tokamak] materials irradiations in the ATR I1 position

    International Nuclear Information System (INIS)

    Rogers, J.W.; Anderl, R.A.

    1989-12-01

    Measurements were performed to help characterize the neutron environments in which the Compact Ignition Tokamak (CIT) materials were irradiated. These materials were irradiated in a lead shield plug assembly at the ATR I1 position. Neutron monitor materials were placed in the capsules in proximity with the CIT specimens. The neutron monitors sensed the neutrons through reactions that have different neutron energy region responses. By measuring the radioactivity of the neutron monitors it was possible to determine the neutron fluence rates (n/cm 2 /sec) and fluences (n/cm 2 ) at the locations of the monitors. It was also possible to determine the axial and radial gradients of the neutron environments near the specimens. This report presents the results obtained from these measurements for both the CIT number-sign 1 (ORNL 64-2) and CIT number-sign 2 (ORNL 64-1) capsules. In general, ASTM methods and procedures were used in all neutron monitoring associated activities. 7 refs., 9 figs., 10 tabs

  17. AGR-2 Data Qualification Report for ATR Cycles 147A, 148A, 148B, and 149A

    International Nuclear Information System (INIS)

    Abbott, Michael L.; Daum, Keith A.

    2011-01-01

    This report presents the data qualification status of fuel irradiation data from the first four reactor cycles (147A, 148A, 148B, and 149A) of the on-going second Advanced Gas Reactor (AGR-2) experiment as recorded in the NGNP Data Management and Analysis System (NDMAS). This includes data received by NDMAS from the period June 22, 2010 through May 21, 2011. AGR-2 is the second in a series of eight planned irradiation experiments for the AGR Fuel Development and Qualification Program, which supports development of the very high temperature gas-cooled reactor (VHTR) under the Next Generation Nuclear Plant (NGNP) Project. Irradiation of the AGR-2 test train is being performed at the Advanced Test Reactor (ATR) at the Idaho National Laboratory (INL) and is planned for 600 effective full power days (approximately 2.75 calendar years) (PLN-3798). The experiment is intended to demonstrate the performance of UCO (uranium oxycarbide) and UO2 (uranium dioxide) fuel produced in a large coater. Data qualification status of the AGR-1 experiment was reported in INL/EXT-10-17943 (Abbott et al. 2010).

  18. Adsorption of Lysine on Na-Montmorillonite and Competition with Ca(2+): A Combined XRD and ATR-FTIR Study.

    Science.gov (United States)

    Yang, Yanli; Wang, Shengrui; Liu, Jingyang; Xu, Yisheng; Zhou, Xiaoyun

    2016-05-17

    Lysine adsorption at clay/aqueous interfaces plays an important role in the mobility, bioavailability, and degradation of amino acids in the environment. Knowledge of these interfacial interactions facilitates our full understanding of the fate and transport of amino acids. Here, X-ray diffraction (XRD) and attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR) measurements were used to explore the dynamic process of lysine adsorption on montmorillonite and the competition with Ca(2+) at the molecular level. Density functional theory (DFT) calculations were employed to determine the peak assignments of dissolved lysine in the solution phase. Three surface complexes, including dicationic, cationic, and zwitterionic structures, were observed to attach to the clay edge sites and penetrate the interlayer space. The increased surface coverage and Ca(2+) competition did not affect the interfacial lysine structures at a certain pH, whereas an elevated lysine concentration contributed to zwitterionic-type coordination at pH 10. Moreover, clay dissolution at pH 4 could be inhibited at a higher surface coverage with 5 and 10 mM lysine, whereas the inhibition effect was inconspicuous or undetected at pH 7 and 10. The presence of Ca(2+) not only could remove a part of the adsorbed lysine but also could facilitate the readsorption of dissolved Si(4+) and Al(3+) and surface protonation. Our results provide new insights into the process of lysine adsorption and its effects on montmorillonite surface sites.

  19. Measurement and characterization of external oil in the fried waxy maize starch granules using ATR-FTIR and XRD.

    Science.gov (United States)

    Chen, Long; Tian, Yaoqi; Sun, Binghua; Cai, Canxin; Ma, Rongrong; Jin, Zhengyu

    2018-03-01

    Concerns regarding increased dietary oil uptake have prompted efforts to investigate the oil absorption and distribution in fried starchy foods. In the present study, attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, together with a chloroform-methanol method, was used to analyze the external and internal oil contents in fried starchy samples. The micromorphology of fried starchy samples was further investigated using scanning electron microscope (SEM), polarized light microscope (PLM) and confocal laser scanning microscopy (CLSM). The results indicated that large amounts of oil were absorbed in or within waxy maize starch, but the majority of oil was located near the surface layer of the starch granules. After defatting, the internal oil was thoroughly removed, while a small amount of external oil remained. As evidenced by the changes of the crystalline characteristics with the help of X-ray diffraction (XRD), the interaction between starch and lipids on the surface was confirmed to form V-type complex compounds during frying at high moisture. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Determination of surface concentrations of individual molecule-layers used in nanoscale biosensors by in situ ATR-FTIR spectroscopy

    KAUST Repository

    Punzet, Manuel

    2012-01-01

    For the development of nanowire sensors for chemical and medical detection purposes, the optimal functionalization of the surface is a mandatory component. Quantitative ATR-FTIR spectroscopy was used in situ to investigate the step-by-step layer formation of typical functionalization protocols and to determine the respective molecule surface concentrations. BSA, anti-TNF-α and anti-PSA antibodies were bound via 3-(trimethoxy)butylsilyl aldehyde linkers to silicon-oxide surfaces in order to investigate surface functionalization of nanowires. Maximum determined surface concentrations were 7.17 × 10 -13 mol cm -2 for BSA, 1.7 × 10 -13 mol cm -2 for anti-TNF-α antibody, 6.1 × 10 -13 mol cm -2 for anti-PSA antibody, 3.88 × 10 -13 mol cm -2 for TNF-α and 7.0 × 10 -13 mol cm -2 for PSA. Furthermore we performed antibody-antigen binding experiments and determined the specific binding ratios. The maximum possible ratio of 2 was obtained at bulk concentrations of the antigen in the μg ml -1 range for TNF-α and PSA. © 2012 The Royal Society of Chemistry.

  1. Total sulfur determination in residues of crude oil distillation using FT-IR/ATR and variable selection methods

    Science.gov (United States)

    Müller, Aline Lima Hermes; Picoloto, Rochele Sogari; Mello, Paola de Azevedo; Ferrão, Marco Flores; dos Santos, Maria de Fátima Pereira; Guimarães, Regina Célia Lourenço; Müller, Edson Irineu; Flores, Erico Marlon Moraes

    2012-04-01

    Total sulfur concentration was determined in atmospheric residue (AR) and vacuum residue (VR) samples obtained from petroleum distillation process by Fourier transform infrared spectroscopy with attenuated total reflectance (FT-IR/ATR) in association with chemometric methods. Calibration and prediction set consisted of 40 and 20 samples, respectively. Calibration models were developed using two variable selection models: interval partial least squares (iPLS) and synergy interval partial least squares (siPLS). Different treatments and pre-processing steps were also evaluated for the development of models. The pre-treatment based on multiplicative scatter correction (MSC) and the mean centered data were selected for models construction. The use of siPLS as variable selection method provided a model with root mean square error of prediction (RMSEP) values significantly better than those obtained by PLS model using all variables. The best model was obtained using siPLS algorithm with spectra divided in 20 intervals and combinations of 3 intervals (911-824, 823-736 and 737-650 cm-1). This model produced a RMSECV of 400 mg kg-1 S and RMSEP of 420 mg kg-1 S, showing a correlation coefficient of 0.990.

  2. Distinct roles of ATM and ATR in the regulation of ARP8 phosphorylation to prevent chromosome translocations.

    Science.gov (United States)

    Sun, Jiying; Shi, Lin; Kinomura, Aiko; Fukuto, Atsuhiko; Horikoshi, Yasunori; Oma, Yukako; Harata, Masahiko; Ikura, Masae; Ikura, Tsuyoshi; Kanaar, Roland; Tashiro, Satoshi

    2018-05-08

    Chromosomal translocations are hallmarks of various types of cancers and leukemias. However, the molecular mechanisms of chromosome translocations remain largely unknown. The ataxia-telangiectasia mutated (ATM) protein, a DNA damage signaling regulator, facilitates DNA repair to prevent chromosome abnormalities. Previously, we showed that ATM deficiency led to the 11q23 chromosome translocation, the most frequent chromosome abnormalities in secondary leukemia. Here, we show that ARP8, a subunit of the INO80 chromatin remodeling complex, is phosphorylated after etoposide treatment. The etoposide-induced phosphorylation of ARP8 is regulated by ATM and ATR, and attenuates its interaction with INO80. The ATM-regulated phosphorylation of ARP8 reduces the excessive loading of INO80 and RAD51 onto the breakpoint cluster region. These findings suggest that the phosphorylation of ARP8, regulated by ATM, plays an important role in maintaining the fidelity of DNA repair to prevent the etoposide-induced 11q23 abnormalities. © 2018, Sun et al.

  3. Intra-operative on-line discrimination of kidney cancer from normal tissue by IR ATR spectroscopy of extracellular fluid

    Science.gov (United States)

    Urboniene, V.; Velicka, M.; Ceponkus, J.; Pucetaite, M.; Jankevicius, F.; Sablinskas, V.; Steiner, G.

    2016-03-01

    Determination of cancerous and normal kidney tissues during partial, simple or radical nephrectomy surgery was performed by using differences in the IR absorption spectra of extracellular fluid taken from the corresponding tissue areas. The samples were prepared by stamping of the kidney tissue on ATR diamond crystal. The spectral measurements were performed directly in the OR during surgery for 58 patients. It was found that intensities of characteristic spectral bands of glycogen (880-1200 cm-1) in extracellular fluid are sensitive to the type of the tissue and can be used as spectral markers of tumours. Characteristic spectral band of lactic acid (1730 cm-1) - product of the anaerobic glycolysis, taking place in the cancer cells is not suitable for use as a spectral marker of cancerous tissue, since it overlaps with the band of carbonyl stretch in phospholipids and fatty acids. Results of hierarchical cluster analysis of the spectra show that the spectra of healthy and tumour tissue films can be reliably separated into two groups. On the other hand, possibility to differentiate between tumours of different types and grades remains in question. While the fluid from highly malignant G3 tumour tissue contains highly pronounced glycogen spectral bands and can be well separated from benign and G1 tumours by principal component analysis, the variations between spectra from sample to sample prevent from obtaining conclusive results about the grouping between different tumour types and grades. The proposed method is instant and can be used in situ and even in vivo.

  4. Determination of main fruits in adulterated nectars by ATR-FTIR spectroscopy combined with multivariate calibration and variable selection methods.

    Science.gov (United States)

    Miaw, Carolina Sheng Whei; Assis, Camila; Silva, Alessandro Rangel Carolino Sales; Cunha, Maria Luísa; Sena, Marcelo Martins; de Souza, Scheilla Vitorino Carvalho

    2018-07-15

    Grape, orange, peach and passion fruit nectars were formulated and adulterated by dilution with syrup, apple and cashew juices at 10 levels for each adulterant. Attenuated total reflectance Fourier transform mid infrared (ATR-FTIR) spectra were obtained. Partial least squares (PLS) multivariate calibration models allied to different variable selection methods, such as interval partial least squares (iPLS), ordered predictors selection (OPS) and genetic algorithm (GA), were used to quantify the main fruits. PLS improved by iPLS-OPS variable selection showed the highest predictive capacity to quantify the main fruit contents. The selected variables in the final models varied from 72 to 100; the root mean square errors of prediction were estimated from 0.5 to 2.6%; the correlation coefficients of prediction ranged from 0.948 to 0.990; and, the mean relative errors of prediction varied from 3.0 to 6.7%. All of the developed models were validated. Copyright © 2018 Elsevier Ltd. All rights reserved.

  5. Fabrication, inspection, and test plan for the Advanced Test Reactor (ATR) Mixed-Oxide (MOX) fuel irradiation project

    International Nuclear Information System (INIS)

    Wachs, G.W.

    1997-11-01

    The Department of Energy (DOE) Fissile Materials Disposition Materials Disposition Program (FMDP) has announced that reactor irradiation of MOX fuel is one of the preferred alternatives for disposal of surplus weapons-usable plutonium (Pu). MOX fuel has been utilized domestically in test reactors and on an experimental basis in a number of Commercial Light Water Reactors (CLWRs). Most of this experience has been with Pu derived from spent low enriched uranium (LEU) fuel, known as reactor grade (RG) Pu. The MOX fuel test will be irradiated in the ATR to provide preliminary data to demonstrate that the unique properties of surplus weapons-derived or weapons-grade (WG) plutonium (Pu) do not compromise the applicability of this MOX experience base. In addition, the test will contribute experience with irradiation of gallium-containing fuel to the data base required for resolution of generic CLWR fuel design issues (ORNL/MD/LTR-76). This Fabrication, Inspection, and Test Plan (FITP) is a level 2 document as defined in the FMDP LWR MOX Fuel Irradiation Test Project Plan (ORNL/MD/LTR-78)

  6. The Conference Proceedings of the 2001 Air Transport Research Society (ATRS) of the WCTR Society. Volume 2

    Science.gov (United States)

    Lee, Yeong-Heok (Editor); Bowen, Brent D. (Editor); Tarry, Scott E. (Editor)

    2001-01-01

    The ATRS held its 5th Annual conference at the City University of Hong Kong Campus in July 2001. The conference was a success with nearly 140 participants including 70 presenters. Titles that comprise Volume 2 include: Intelligent Airport Gate Assignment System; A Study on the Effects of the Personality Compatibility to the Job Performance; ITS/CVO Application for Air cargo Transportation in Korea; An Airport as a Logistics and Economic Hub: The Case of Incheon International Airport; The Impact Of Aviation Safety over the Consumer's Behavior; The Integration of China and Taiwan Air Networks for Direct Air Cargo Services; Quality perception and carrier choice in Civil Aviation; Future Trends in Business Travel Decision Making; Cooperation Among German Airports in Europe; Inbound and Outbound Air Passenger Traffic Forecasting between the United States and Selected Asian countries; An Evaluation of Alternative Facilities for Airport Redevelopment using Fuzzy Linguistic Approach; Economic Analysis of Airline Alliances; The Aviation Cooperation between the two Koreas Preparing for the Reunification of the Peninsula; and A Study on the Air Transport Cooperation in Northeast Asia between China, Japan and Korea.

  7. Quantification of pure refined olive oil adulterant in extra virgin olive oil using diamond cell atr-ftir spectroscopy

    International Nuclear Information System (INIS)

    Kandhro, A.A.; Saleem, R.; Laghari, A.H.; Sultana, R.

    2014-01-01

    The present study depicts spectroscopic method development to deliver a rapid, simple and reproducible quantification of pure refined olive oil (PROO) adulterant in extra virgin olive oil (EVOO) using partial least square (PLS) regression (statistical parameter). Single bounce attenuated total reflectance (SB-ATR) Fourier transform infrared (FTIR) was choice in the developed method. Blended standards of PROO and EVOO were obtained by their weight by weight percentage and the values were used to construct calibration curves for quantification. The optimum regression values (i.e. >0.99) were achieved using the combined frequencies of 3105-2761, 1838-1687, and 1482-440 cm-1 with regression coefficients (R2) 0.99718 and achieved residual mean square error of calibration (RMSEC) 1.40% w/w. To determine the suitability of developed method principal component spectra (PCS) diagnostic was also used. The results of the present study prove that the developed methods reported in preceding studies can be good option for more rapid and accurate determination of PROO adulteration in EVOO. (author)

  8. ATR-IR spectroscopy for the detection of induced-phase transition in Langmuir-Blodgett monolayer film

    International Nuclear Information System (INIS)

    Widayati, Suci

    1996-01-01

    The rate at which a solid substrate is transferred through the Air/Water interface in the Langmuir-Blodgett process of preparing monomolecular films influences the final structure of the transferred film. This phenomenon has been observed from the attenuated total reflectance infra-red (ATR-IR) spectra of fatty acid monolayer transferred onto germanium substrate. This transfer-induced effect is most evidence when the monolayer is transferred from an expanded region of the surface-pressure-molecular area isotherm, but has limited influence on the hydrocarbon chain conformation of film molecules transferred in the condensed phases at high surface pressure. Such a conformational ordering may due to a kinetically limited phase transition taking place in the meniscus formed between the solid substrate and aqueous sub phase. In addition, these results suggest that the structure of the amphiphilic molecules may modulate the extent and nature of the dipping-speed-induced structural changes taking place in the monomolecular L-B film. In order to use monomolecular L-B films to accurately characterize the structure, orientation and phase properties of monolayers at the Air/Water interface, the L-B transfer must be performed at transfer speeds that minimize this structural phase transition

  9. A Feasibility Study to Determine Cooling Time and Burnup of ATR Fuel Using a Nondestructive Technique and Three Types of Gamma-ray Detectors

    International Nuclear Information System (INIS)

    Navarro, Jorge; Aryaeinejad, Rahmat; Nigg, David W.

    2011-01-01

    Effective and efficient Advanced Test Reactor (ATR) fuel management require state of the art core modeling tools. These new tools will need isotopic and burnup validation data before they are put into production. To create isotopic, burn up validation libraries and to determine the setup for permanent fuel scanner system a feasibility study was perform. The study consisted in measuring short and long cooling time fuel elements at the ATR canal. Three gamma spectroscopy detectors (HPGe, LaBr3, and HPXe) and two system configurations (above and under water) were used in the feasibility study. The first stage of the study was to investigate which detector and system configuration would be better suited for different scenarios. The second stage of the feasibility study was to create burnup and cooling time calibrations using experimental isotopic data collected and ORIGEN 2.2 burnup data. The results of the study establish that a better spectra resolution is achieve with an above the water configuration and that three detectors can be used in the permanent fuel scanner system for different situations. In addition it was conclude that a number of isotopic ratios and absolute measurements could be used to predict ATR fuel burnup and cooling times.

  10. Determination of alkaloids in capsules, milk and ethanolic extracts of poppy (Papaver somniferum L.) by ATR-FT-IR and FT-Raman spectroscopy.

    Science.gov (United States)

    Schulz, Hartwig; Baranska, Malgorzata; Quilitzsch, Rolf; Schütze, Wolfgang

    2004-10-01

    Fourier transform (FT) infrared spectroscopy using a diamond composite ATR crystal and NIR-FT-Raman spectroscopy techniques were applied for the simultaneous identification and quantification of the most important alkaloids in poppy capsules. Most of the characteristic Raman signals of the alkaloids can be identified in poppy milk isolated from unripe capsules. But also poppy extracts present specific bands relating clearly to the alkaloid fraction. Raman spectra obtained by excitation with a Nd:YAG laser at 1064 nm show no disturbing fluorescence effects; therefore the plant tissue can be recorded without any special preparation. The used diamond ATR technique allows to measure very small sample amounts (5-10 microL or 2-5 mg) without the necessity to perform time-consuming pre-treatments. When applying cluster analysis a reliable discrimination of "low-alkaloid" and "high-alkaloid" poppy single-plants can be easily achieved. The examples presented in this study provide clear evidence of the benefits of Raman and ATR-IR spectroscopy in efficient quality control, forensic analysis and high-throughput evaluation of poppy breeding material.

  11. A new Density Functional Theory (DFT) based method for supporting the assignment of vibrational signatures of mannan and cellulose—Analysis of palm kernel cake hydrolysis by ATR-FT-IR spectroscopy as a case study

    DEFF Research Database (Denmark)

    Barsberg, Søren Talbro; Sanadi, Anand Ramesh; Jørgensen, Henning

    2011-01-01

    Attenuated Total Reflectance (ATR) FT-IR spectroscopy gives in situ information on molecular concentration, organization and interactions in plant cell walls. We demonstrate its potential for further developments by a case study which combines ATR-FT-IR spectroscopy with a recently published DFT...... a decreasing degree of polymerization to be a plausible cause, although others may interfere. Keywords: Cellulose; Mannan; FT-IR; DFT; Molecular modelling; Palm kernel...

  12. PENGENALAN PENY AKIT NODA PADA CITRA DAUN TEBU BERDASARKAN CIRI TEKSTUR FRACTAL DIMENSION CO-OCCURRENCE MATRIX DAN L*a*b* COLOR MOMENTS

    Directory of Open Access Journals (Sweden)

    Evy Kamilah Ratnasari

    2014-07-01

    Full Text Available Penyakit yang menyerang tebu dapat disebabkan oleh bakteri, jamur maupun virus. Penyakit noda merupakan penyakit pada tanaman tebu yang disebabkan oleh jamur dengan menampakkan lesi atau bercak pada permukaan daun. Penyakit noda tersebut dapat menghambat proses fotosintesis yang akan berakibat menurunkan produksi gula karena mempengaruhi pertumbuhan tebu. Upaya pengendalian dini dapat dilakukan dengan mengenali jenis penyakit melalui lesinya yang bermanfaat dalam menentukan tindakan penanganan yang tepat. Lesi yang disebabkan oleh penyakit noda masing-masing dapat dikenali secara visual karena memiliki ciri warna dan tekstur yang unik. Tetapi pengamatan secara visual memiliki beberapa kekurangan seperti subjektifitas dan kurang akurat. Penelitian ini mengusulkan pengenalan penyakit noda tanaman tebu yang terdiri dari noda cincin, noda karat, dan noda kuning berdasarkan fitur tekstur yang merupakan kombinasi dari konsep Gray Level Co-Occurrence Matrix (GLCM dan dimensi fraktal yang dinamakan Fractal Dimension Co-Occurrence Matrix (FDCM. Sedangkan fitur warna didapatkan dari perhitungan statistik col or moments pada citra L*a*b*. Kombinasi fitur tersebut menghasilkan 12 fitur warna dan 6 fitur tekstur yang kemudian digunakan sebagai masukan klasifikasi k-Nearest Neighbor (KNN. Pengenalan penyakit noda pada tanaman tebu menggunakan metode tersebut dapat menghasilkan akurasi tertinggi 90%.

  13. Pembuatan Service Level Requirement, Service Level Agreement dan Operational Level Agreement pada layanan help desk SAP berdasarkan kerangka kerja ITIL versi 2011 (Studi Kasus : Pupuk Indonesia Holding Company

    Directory of Open Access Journals (Sweden)

    Nur Shabrina Prameswari

    2017-01-01

    Full Text Available PT. Pupuk Indonesia Holding Company baru saja melakukan implementasi SAP pada tahun 2014. Dalam penerapannya, perusahaan merasa perlu membuat help desk SAP sebagai pusat penanganan masalah bagi perusahaan dan 7 anak perusahaannya yang kemudian dapat berfungsi juga sebagai knowledge base yang berguna apabila ada masalah yang berulang diwaktu selanjutnya. Untuk merancang layanan help desk yang baik, perlu didefinisikannya target layanan dalam sebuah kontrak perjanjian antara pengguna layanan dan penyedia layanan. Selain itu,Perjanjian layanan tersebut diperlukan juga sebagai jaminan kualitas help desk yang dapat disepakati oleh penyedia layanan dan pengguna layanan yang merupakan pengguna SAP pada PT. Pupuk Indonesia dan anak perusahaannya. Hal tersebut bertujuan untuk menyelaraskan bisnis dengan kualitas layanan serta menentukan kebutuhan dan harapan pelanggan dalam sebuah perjanjian antara penyedia layanan dan pengguna layanan. Dari permasalahan tersebut, maka diperlukan pembuatan dokumen Service Level Requirement, Service Level Agreement dan juga Operational Level Agreement pada help desk SAP, dengan dilakukan observasi dokumen dan wawancara pada pihak pengguna layanan dan penyedia layanan, maka setelah itu dibuatlah dokumen Service Level management tersebut berdasarkan ITIL Versi 2011.

  14. Pembuatan Standard Operating Procedure (SOP Service Desk Berdasarkan Kerangka Kerja Itil V3 dengan Menggunakan Metode Analisis Gap Layanan (Studi Kasus: PT. XYZ , Tangerang

    Directory of Open Access Journals (Sweden)

    Annisa Rachmi

    2014-09-01

    Full Text Available Penggunaan Teknologi Informasi di banyak perusahaan telah menjadi satu hal penting dalam meningkatkan efektifitas dan efisiensi operasional bisnis yang mendukung tercapainya tujuan perusahaan, termasuk juga PT XYZ. Unit fungsional service desk sangat dibutuhkan oleh PT XYZ dalam mendukung operasional TI dan menangani permasalahan yang muncul serta memonitor terkait penggunaan TI yang ada di dalam perusahaan. Adanya kebutuhan PT XYZ untuk menjadikan service desk menjadi unit yang dapat berjalan dengan baik sesuai dengan fungsinya, menunjukkan bahwa perlunya penerapan tata kelola TI untuk mengatur dan mengelola aktivitas service desk. Salah satu tata kelola TI yang dimaksud adalah berupa prosedur kerja. Kerangka kerja Information Technology Infrastructure Library (ITIL merupakan suatu best practice yang bertujuan secara berkelanjutan meningkatkan efisiensi operasional TI. Perancangan dan implementasi service desk berdasarkan kerangka kerja ITIL V3 di PT XYZ diperlukan dalam upaya meningkatkan layanan TI dan mengatasi masalah yang ada saat ini. Pembuatan SOP ini diinisialisasi dengan menggunakan metode analisis gap, proses-proses yang dimuat ke dalam SOP merupakan hasil analisis kesenjangan antara kondisi kekinian service desk PT XYZ dengan kondisi ideal service desk framework ITIL.

  15. EVALUASI KEAMANAN INFORMASI PADA PTI PDAM TIRTA MOEDAL KOTA SEMARANG BERDASARKAN INDEKS KEAMANAN INFORMASI SNI ISO/IEC 27001:2009

    Directory of Open Access Journals (Sweden)

    Diah Restu Wardani

    2015-10-01

    Full Text Available Adanya rencana untuk meningkatkan keamanan informasi sesuai dengan Standar Nasional Indonesia (SNI. Selain rencana tersebut, ditemukan kurangnya kesadaran dari masing-masing staff PTI PDAM Tirta Moedal Kota Semarang terkait dengan keamanan informasi menyebabkan terjadinya berbagai masalah salah satunya penyalahgunaan wewenang yang dilakukan oleh staff PTI yaitu dengan mengubah sistem operasi yang telah ditetapkan oleh PTI PDAM dan kejadian tersebut berdampak pada proses bisnis. Ditemukan pula bahwa kurangnya pengamanan pada ruang server yang ada di divisi PTI. Tujuan dari penelitian ini adalah untuk mengevaluasi tingkat kelengkapan dan tingkat kematangan keamanan informasi pada divisi PTI serta memberikan rekomendasi perbaikan untuk meningkatkan tingkat kelengkapan dan tingkat kematangan keamanan informasi. Dari hasil studi dokumen, wawancara, dan kuesioner berdasarkan Indeks Keamanan Informasi SNI ISO/IEC 27001:2009 dihasilkan tingkat kelengkapan keamanan informasi sebesar 325 dan tingkat kematangan berada pada tingkat I+ dimana sudah ada pemahaman mengenai perlunya pengelolaan kemanan informasi akan tetapi penerapan langkah pengamanan masih belum teratur. Untuk mencapai tingkat kematangan V dan sesuai dengan Standar Nasional Indonesia (SNI PTI PDAM Tirta Moedal Kota Semarang dapat melakukan strategi perbaikan secara bertahap. Kata Kunci: Indeks Keamanan Informasi, SNI ISO/IEC 27001:2009, Evaluasi Keamanan Informasi, Tingkat Kelengkapan, Tingkat Kematangan

  16. APLIKASI SISTEM INFORMASI PERTANAHAN BERDASARKAN JENIS-JENIS HAK ATAS TANAH DI KANTOR PERTANAHAN KOTA SURABAYA II (STUDI KASUS KELURAHAN GENTENG, KECAMATAN GENTENG, SURABAYA

    Directory of Open Access Journals (Sweden)

    Ery Abdul Baary

    2015-02-01

    Full Text Available Di Kantor Pertanahan Kota Surabaya II, sistem informasi pertanahan belum diterapkan, jadi untuk melaksanakan tugasnya hanya menggunakan sebatas peta digital, maka dari itu perlu adanya sistem informasi pertanahan yang terpadu dan mutakhir untuk mendukung dan mengoptimalkan pengelolaan sistem informasi pertanahan di Kota Surabaya II.Pada penelitian ini akan dilakukan pembuatan sistem informasi pertanahan berdasarkan jenis hak atas tanah dengan menggunakan peta digital Kelurahan Genteng, Kecamatan Genteng, Surabaya skala 1:1000, citra Quickbird 2010, database mengenai bidang tanah dan data hasil survey. Pengolahan data menggunakan software utama Autodesk Land Dekstop 2009, ArcGIS 9.3. Pembuatan program aplikasi menggunakan software Visual Basic 6.0 yang dilengkapi dengan software tambahan MapObject 2.2.Dari hasil pembuatan Sistem informasi pertanahan di Kelurahan Genteng, Program ini mampu mengidentifikasi informasi bidang tanah mengenai jenis hak atas tanah yang meliputi 224 bidang tanah yang terdiri dari 132 bidang tanah atas hak guna bangunan, 89 bidang tanah atas hak milik, dan 3 bidang tanah atas hak pakai, serta dilengkapi dengan buku panduan sehingga dapat digunakan untuk membantu dalam pengambilan kebijakan dalam rencana pelaksanaan program inventarisasi penguasaan, pemilikan, penggunaan, dan pemanfaatan tanah.

  17. Arecoline-induced phosphorylated p53 and p21(WAF1) protein expression is dependent on ATM/ATR and phosphatidylinositol-3-kinase in clone-9 cells.

    Science.gov (United States)

    Chou, Wen-Wen; Guh, Jinn-Yuh; Tsai, Jung-Fa; Hwang, Chi-Ching; Chiou, Shean-Jaw; Chuang, Lea-Yea

    2009-06-01

    Betel-quid use is associated with liver cancer whereas its constituent arecoline is cytotoxic, genotoxic, and induces p53-dependent p21(WAF1) protein expression in Clone-9 cells (rat hepatocytes). The ataxia telangiectasia mutated (ATM)/rad3-related (ATR)-p53-p21(WAF1) and the phosphatidylinositol-3-kinase (PI3K)-mammalian target of rapamycin (mTOR) pathways are involved in the DNA damage response and the pathogenesis of cancers. Thus, we studied the role of ATM/ATR and PI3K in arecoline-induced p53 and p21(WAF1) protein expression in Clone-9 cells. We found that arecoline (0.5 mM) activated the ATM/ATR kinase at 30 min. The arecoline-activated ATM/ATR substrate contained p-p53Ser15. Moreover, arecoline only increased the levels of the p-p53Ser6, p-p53Ser15, and p-p53Ser392 phosphorylated p53 isoforms among the known isoforms. ATM shRNA attenuated arecoline-induced p-p53Ser15 and p21(WAF1) at 24 h. Arecoline (0.5 mM) increased phosphorylation levels of p-AktSer473 and p-mTORSer2448 at 30-60 min. Dominant-negative PI3K plasmids attenuated arecoline-induced p21(WAF1), but not p-p53Ser15, at 24 h. Rapamycin attenuated arecoline-induced phosphrylated p-p53Ser15, but not p21(WAF1), at 24 h. ATM shRNA, but not dominant-negative PI3K plasmids, attenuated arecoline-induced p21(WAF1) gene transcription. We conclude that arecoline activates the ATM/ATR-p53-p21(WAF1) and the PI3K/Akt-mTOR-p53 pathways in Clone-9 cells. Arecoline-induced phosphorylated p-p53Ser15 expression is dependent on ATM whereas arecoline-induced p21(WAF1) protein expression is dependent on ATM and PI3K. Moreover, p21(WAF1) gene is transcriptionally induced by arecoline-activated ATM. (c) 2009 Wiley-Liss, Inc.

  18. Kinetics study of heterogeneous reactions of ozone with erucic acid using an ATR-IR flow reactor.

    Science.gov (United States)

    Leng, Chunbo; Hiltner, Joseph; Pham, Hai; Kelley, Judas; Mach, Mindy; Zhang, Yunhong; Liu, Yong

    2014-03-07

    The ozone initiated heterogeneous oxidation of erucic acid (EA) thin film was investigated using a flow system combined with attenuated total reflection infrared spectroscopy (ATR-IR) over wide ranges of ozone concentrations (0.25-60 ppm), thin film thickness (0.1-1.0 μm), temperatures (263-298 K), and relative humidities (0-80% RH) for the first time. Pseudo-first-order rate constants, kapp, and overall reactive uptake coefficients, γ, were obtained through changes in the absorbance of C[double bond, length as m-dash]O stretching bands at 1695 cm(-1), which is assigned to the carbonyl group in carboxylic acid. Results showed that the reaction followed the Langmuir-Hinshelwood mechanism and kapp was largely dominated by surface reaction over bulk phase reaction. In addition, both the kapp and the γ values showed very strong temperature dependences (∼two orders of magnitude) over the temperature range; in contrast, they only slightly increased with increasing RH values from 0-80%. According to the kapp values as a function of temperature, the activation energy for the heterogeneous reaction was estimated to be 80.6 kJ mol(-1). Our results have suggested that heterogeneous reactions between ozone and unsaturated solid surfaces likely have a substantially greater temperature dependence than liquid ones. Moreover, the hygroscopic properties of EA thin films before and after exposure to ozone were also studied by measurement of water uptake. Based on the hygroscopicity data, the insignificant RH effect on reaction kinetics was probably due to the relatively weak water uptake by the unreacted and reacted EA thin films.

  19. Aerosol Study over the Gulf of Guinea Region during DACCIWA Using a Mini Lidar onboard the French Aircraft ATR42

    Science.gov (United States)

    Shang, X.; Chazette, P.; Flamant, C.; Totems, J.; Denjean, C.; Meynadier, R.; Perrin, T.; Laurens, M.

    2016-12-01

    The EU-funded project DACCIWA (Dynamics-Aerosol-Chemistry-Cloud Interactions in West Africa) aims to investigate the relationship between weather, climate and air pollution in southern West Africa. As part of this campaign, three research aircraft based in Lomé (Togo) flew targeted missions over West Africa from 27 June to 16 July 2016. In this area aerosols, having a mixing of natural and anthropogenic sources, exert an important influence on the local weather and climate, mainly due to the aerosol-cloud interactions. A mini backscattered lidar system onboard one research aircraft (the French aircraft ATR42) performed aerosols measurements over the Gulf of Guinea region. The main objective was to study aerosol properties in different chemical landscapes: from the background state over the Gulf of Guinea (marine aerosols or mix between marine aerosols and biomass burning aerosols) to ship/flaring emissions to the coastal strip of polluted megacities to the agricultural areas and forest areas further north, and eventually dust from Sahel/Sahara. Different aerosol origins were identified by using the coupling between the lidar cross-polarized channels and a set of back trajectories analyses. The aircraft conducted flights at low ( 1 km above the mean sea level -amsl) and high altitudes ( 5 km amsl), allowing the coupling of in situ and remote sensing data to assess the properties of the aerosol layers. During several flights, depolarizing aerosol layers from the northeast were observed between 2.5 and 4 km amsl, which highlight the significant contribution of dust-like particles to the aerosol load in the coastal region. The air masses originating from the southeast were loaded with biomass burning aerosols from Central Africa, which seem to be mixed with other aerosol types. The flight sampling strategy and related lidar investigations will be presented. The retrieved aerosol distributions and properties, and the aerosol type identification will be discussed.

  20. Study in vitro of Er,Cr:YSGG laser effects in bone tissue by ATR-FTIR spectroscopy

    International Nuclear Information System (INIS)

    Benetti, Carolina

    2010-01-01

    Laser proves to be, more and more, an effective tool for helping health professionals, being intensively used in ophthalmological and odontological procedures. In particular, high-density, infrared emitting lasers have great potential in cutting mineralized biological hard tissues, given their high absorption by hydroxyapatite and water, these tissues' main components. In comparison to mechanical instruments, laser presents a series of advantages, namely, smaller damage to the remaining tissue and promotion of homeostatic effect, apart from making it possible to execute procedures in areas with difficult access. However, for an efficient and safe use of this technique, it is necessary to know the effects of the laser irradiation on the tissue. The Fourier transform infrared (FTIR) technique is heavily used in the study of organic materials, because apart from making it possible to identify the materials' components, it also allows to prepare a semi quantitative analysis. This work aims to establish the ATR-FTIR technique in the characterization of natural and irradiated osseous tissue, and to verify the possible chemical and structural changes caused by irradiation. Firstly, the best conditions for the obtainment of bone sample spectra were determined. Then, bone samples, irradiated with the Er,Cr:YSGG (2,78 μm) infrared emitting laser (adjusted with different energy densities) were analyzed alongside with natural bone samples. It has been verified that the technique is effective in the bone tissue characterization, and that it is possible to observe the chemical changes caused by the temperature rise due to laser irradiation. It has been observed a gradual organic material loss as the energy density goes up. These results are the first steps in testing the Er,Cr:YSGG laser efficacy as a cutting tool, a pivotal aspect of its consolidation in clinical procedures. (author)

  1. Housing maintenance of primary cooling pump I of Kartini reactor

    International Nuclear Information System (INIS)

    Agung Nugroho; Wahyu Imam W

    2013-01-01

    Housing maintenance of Primary Cooling Pump have been done with purpose to enhance capability of fluid block and stopping leakage. The procedures of modification are follow: Replace mechanical seal type Nock 560 – 1 ½'' by mechanical seal type Nock 560 - 38, modification gland plate/housing with to lathe the diameter of gland plate/housing from diameter of 54 mm to 60 mm, in size setting-up mechanical seal, alignment and then function test. The result of the modification are: mechanical seal has been installed, housing has been modified, and the leakage of primary cooling water has been repaired and operated properly. Conclusion of the maintenance are the primary cooling water pump is working well, because the primary cooling water is not leakage any more. (author)

  2. Penentuan Batas Pengelolaan Wilayah Laut Antara Provinsi Jawa Timur dan Provinsi Bali Berdasarkan Undang-Undang Republik Indonesia Nomor 23 Tahun 2014

    Directory of Open Access Journals (Sweden)

    Rainhard Sumarto Simatupang

    2017-01-01

    pengelolaan wilayah laut daerah sesuai lokasi penelitian ini yang sudah berdasarkan UU No. 23 Tahun 2014 

  3. ATR-p53 restricts homologous recombination in response to replicative stress but does not limit DNA interstrand crosslink repair in lung cancer cells.

    Directory of Open Access Journals (Sweden)

    Bianca M Sirbu

    Full Text Available Homologous recombination (HR is required for the restart of collapsed DNA replication forks and error-free repair of DNA double-strand breaks (DSB. However, unscheduled or hyperactive HR may lead to genomic instability and promote cancer development. The cellular factors that restrict HR processes in mammalian cells are only beginning to be elucidated. The tumor suppressor p53 has been implicated in the suppression of HR though it has remained unclear why p53, as the guardian of the genome, would impair an error-free repair process. Here, we show for the first time that p53 downregulates foci formation of the RAD51 recombinase in response to replicative stress in H1299 lung cancer cells in a manner that is independent of its role as a transcription factor. We find that this downregulation of HR is not only completely dependent on the binding site of p53 with replication protein A but also the ATR/ATM serine 15 phosphorylation site. Genetic analysis suggests that ATR but not ATM kinase modulates p53's function in HR. The suppression of HR by p53 can be bypassed under experimental conditions that cause DSB either directly or indirectly, in line with p53's role as a guardian of the genome. As a result, transactivation-inactive p53 does not compromise the resistance of H1299 cells to the interstrand crosslinking agent mitomycin C. Altogether, our data support a model in which p53 plays an anti-recombinogenic role in the ATR-dependent mammalian replication checkpoint but does not impair a cell's ability to use HR for the removal of DSB induced by cytotoxic agents.

  4. Influence of alkali metal cations/type of activator on the structure of alkali-activated fly ash - ATR-FTIR studies

    Science.gov (United States)

    Król, M.; Rożek, P.; Chlebda, D.; Mozgawa, W.

    2018-06-01

    Coal fly ash as a secondary aluminosiliceous raw material that is commonly used in the so-called geopolymerization process has been activated with different alkali hydroxides solutions: LiOH, NaOH and KOH. Changes in the aluminosilicate structure of the material during alkali-activation have been analyzed in detail on the basis of ATR/FT-IR spectra. These changes mainly affect both the integral intensity and FWHM of bands in the range of 1200-950 cm-1, however dehydration and carbonation process can be also analyzed based on obtaining results.

  5. ATR-FTIR and Raman spectroscopic investigation of the electroporation-mediated transdermal delivery of a nanocarrier system containing an antitumour drug.

    Science.gov (United States)

    Balázs, Boglárka; Sipos, Péter; Danciu, Corina; Avram, Stefana; Soica, Codruta; Dehelean, Cristina; Varju, Gábor; Erős, Gábor; Budai-Szűcs, Mária; Berkó, Szilvia; Csányi, Erzsébet

    2016-01-01

    The aim of the present work was the optimization of the transdermal delivery of a lyotropic liquid crystal genistein-based formulation (LLC-GEN). LLC was chosen as medium in view of the poor solubility of GEN in water. Membrane diffusion and penetration studies were carried out with a Franz diffusion cell, through a synthetic membrane in vitro, a chick chorioallantoic membrane ex ovo, and ex vivo excised human epidermis. Thereafter, LLC-GEN was combined with electroporation (EP) to enhance the transdermal drug delivery. The synergistic effect of EP was verified by in vivo ATR-FTIR and ex vivo Raman spectroscopy on hairless mouse skin.

  6. El diálogo creativo en El cuarto de atrás : La búsqueda de una identidad en la memoria colectiva

    OpenAIRE

    Muñoz Åhlén, Anna-Maria

    2017-01-01

    Esta tesina tiene como objetivo analizar la relación entre C. y el interlocutor misterioso en El cuarto de atrás de Carmen Martín Gaite. Intentaremos averiguar qué recuerdos aparecen por medio del diálogo entre los interlocutores. Partiendo de allí, el trabajo enfocará qué recuerdos de infancia aparecen por medio del diálogo y de qué manera han influido en el personaje C. en el tiempo real de la creación de la novela. Como apoyo de interpretar la identidad del personaje nos apoyaremos princip...

  7. ATR-Chk1-APC/C-dependent stabilization of Cdc7-ASK (Dbf4) kinase is required for DNA lesion bypass under replication stress

    DEFF Research Database (Denmark)

    Yamada, M.; Watanabe, K.; Mistrik, M.

    2013-01-01

    replication. Stalled DNA replication evoked stabilization of the Cdc7-ASK (Dbf4) complex in a manner dependent on ATR-Chk1-mediated checkpoint signaling and its interplay with the anaphase-promoting complex/cyclosomeCdh1 (APC/C) ubiquitin ligase. Mechanistically, Chk1 kinase inactivates APC/C through...... degradation of Cdh1 upon replication block, thereby stabilizing APC/C substrates, including Cdc7-ASK (Dbf4). Furthermore, motif C of ASK (Dbf4) interacts with the N-terminal region of RAD18 ubiquitin ligase, and this interaction is required for chromatin binding of RAD18. Impaired interaction of ASK (Dbf4...

  8. Glycation in human fingernail clippings using ATR-FTIR spectrometry, a new marker for the diagnosis and monitoring of diabetes mellitus.

    Science.gov (United States)

    Coopman, Renaat; Van de Vyver, Thijs; Kishabongo, Antoine Sadiki; Katchunga, Philippe; Van Aken, Elisabeth H; Cikomola, Justin; Monteyne, Tinne; Speeckaert, Marijn M; Delanghe, Joris R

    2017-01-01

    Although HbA1c is a good diagnostic tool for diabetes, the precarity of the health system and the costs limit the use of this biomarker in developing countries. Fingernail clippings contain ±85% of keratins, which are prone to glycation. Nail keratin glycation may reflect the average glycemia over the last months. We explored if attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) can be used as a non-invasive tool for assessing glycation in diabetes. Using ATR-FTIR spectroscopy, glycation and deglycation experiments with fructosamine 3-kinase allowed to identify the spectrum that corresponds with keratin glycation in fingernail clippings. Clippings of 105 healthy subjects and 127 diabetics were subjected to the standardized ATR-FTIR spectroscopy method. In vitro glycation resulted in an increased absorption at 1047cm -1 . Following enzymatic deglycation, this peak diminished significantly, proving that the AUC between 970 and 1140cm -1 corresponded with glycated proteins. Within-run CV of the assay was 3%. Storage of nail clippings at 37°C for 2weeks did not significantly change results. In diabetics, glycated nail protein concentrations (median: 1.51μmol/g protein, IQR: 1.37-1.85μmol/g protein) were significantly higher than in the controls (median: 1.19μmol/g protein, IQR: 1.09-1.26μmol/g protein) (p<0.0001). ROC analysis yielded an AUC of 0.92 at a cut-off point of 1.28μmol/g nail (specificity: 82%; sensitivity: 90%). No correlation was observed between the glycated nail protein concentrations and HbA1c. Protein glycation analysis in fingernails with ATR-FTIR spectroscopy could be an alternative affordable technique for diagnosing and monitoring diabetes. As the test does not consume reagents, and the preanalytical phase is extremely robust, the test could be particularly useful in developing countries. Copyright © 2016 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  9. Role of Bi promotion and solvent in platinum-catalyzed alcohol oxidation probed by in situ X-ray absorption and ATR-IR spectroscopy

    DEFF Research Database (Denmark)

    Mondelli, C.; Grunwaldt, Jan-Dierk; Ferri, D.

    2010-01-01

    the catalysts under working conditions using in situ X-ray absorption spectroscopy (XAS) and attenuated total reflection infrared spectroscopy (ATR-IR), aiming at uncovering the roles of the metal promoter and the reaction medium. XAS confirms that Bi is oxidized more easily than Pt, maintaining the catalytic...... surfaces than on step or kink sites. Side products, CO and benzoate species, appearing during the reaction reveal that the geometric suppression of undesired reactions does not occur to the same extent on Pt-based catalysts as on Pd, suggesting that decarbonylation of the produced aldehyde on Pt may occur...

  10. Aplicación de las espectroscopias IR/ATR y Raman al estudio de la superficie de vidrios sometidos a molturación

    Directory of Open Access Journals (Sweden)

    Pérez-Villar, S.

    2008-04-01

    Full Text Available The grinding process of vítreous silica and a conventional glass has been studied by means of Raman and IR/ATR spectroscopies. By using the obtained spectra, the positions and areas of the different bands have been calculated. The Raman spectra have been analysed using the Polymerisation Index (PI that indicates the depolymerization degree achieved on the particle surface after grinding. On the other hand, the IR/ATR spectra have been used to follow the positions of the symmetric and antisymmetric Si-O-Si bands. It has been observed that both techniques give similar results. These indicate that the depolymerisation degree increases with the grinding process. This depolymerisation is due to the broke of Si-O-Si bonds.

    El proceso de molienda de sílice vítrea y un vidrio convencional se ha estudiado mediante espectroscopias IR/ATR y Raman. A partir de los espectros obtenidos para diferentes tamaños de partícula se calcularon tanto las posiciones como las áreas de las bandas que aparecían en cada espectro. Utilizando los espectros Raman se ha hecho uso del denominado Índice de Polimerización (IP el cual es indicativo del grado de despolimerización producido en la superficie de las partículas tras el proceso de molienda. Por otro lado, mediante los espectros IR/ATR y analizando las posiciones de las bandas de tensión simétrica y asimétrica de los enlaces Si-O-Si se ha podido seguir el citado proceso de molturación. Se ha observado, mediante ambas técnicas, como la superficie de las partículas se despolimeriza al disminuir el tamaño de partícula. Esta despolimerización es debida a la rotura de los enlaces Si-O-Si.

  11. Human monocytes undergo excessive apoptosis following temozolomide activating the ATM/ATR pathway while dendritic cells and macrophages are resistant.

    Directory of Open Access Journals (Sweden)

    Martina Bauer

    Full Text Available Immunodeficiency is a severe therapy-limiting side effect of anticancer chemotherapy resulting from sensitivity of immunocompetent cells to DNA damaging agents. A central role in the immune system is played by monocytes that differentiate into macrophages and dendritic cells (DCs. In this study we compared human monocytes isolated from peripheral blood and cytokine matured macrophages and DCs derived from them and assessed the mechanism of toxicity of the DNA methylating anticancer drug temozolomide (TMZ in these cell populations. We observed that monocytes, but not DCs and macrophages, were highly sensitive to the killing effect of TMZ. Studies on DNA damage and repair revealed that the initial DNA incision was efficient in monocytes while the re-ligation step of base excision repair (BER can not be accomplished, resulting in an accumulation of DNA single-strand breaks (SSBs. Furthermore, monocytes accumulated DNA double-strand breaks (DSBs following TMZ treatment, while DCs and macrophages were able to repair DSBs. Monocytes lack the DNA repair proteins XRCC1, ligase IIIα and PARP-1 whose expression is restored during differentiation into macrophages and DCs following treatment with GM-CSF and GM-CSF plus IL-4, respectively. These proteins play a key role both in BER and DSB repair by B-NHEJ, which explains the accumulation of DNA breaks in monocytes following TMZ treatment. Although TMZ provoked an upregulation of XRCC1 and ligase IIIα, BER was not enhanced likely because PARP-1 was not upregulated. Accordingly, inhibition of PARP-1 did not sensitize monocytes, but monocyte-derived DCs in which strong PARP activation was observed. TMZ induced in monocytes the DNA damage response pathways ATM-Chk2 and ATR-Chk1 resulting in p53 activation. Finally, upon activation of the Fas-receptor and the mitochondrial pathway apoptosis was executed in a caspase-dependent manner. The downregulation of DNA repair in monocytes, resulting in their selective

  12. ATR-FTIR Based Pre and Post Formulation Compatibility Studies for the Design of Niosomal Drug Delivery System Containing Nonionic Amphiphiles and Chondroprotective Drug

    International Nuclear Information System (INIS)

    Khan, M.I.; Madni, A.; Ahmad, S.; Rehmanand, M.; Mahmood, M.A.; Khan, A.

    2015-01-01

    Pharmaceutical compatibility studies are considered as the most important and first screening stage during development of pharmaceutical drug product. Attenuated total reflectance/fourier transform infrared (ATR-FTIR) is one of the techniques currently available to pharmaceutical scientists for investigating the compatibilities between active drug and inactive pharmaceutical ingredients. The present study was designed to assess the interaction among different niosomes forming components i.e nonionic amphiphiles and chondroprotective/antiinflamatory drug Diacerein by ATR-FTIR method. Physical mixtures and niosomes were prepared by physical mixing and thin film hydration method, respectively. The individual niosomal components, physical mixtures as well as niosomal formulations were analyzed. The spectra of Diacerein showed characteristic peaks at 3300 cm/sup -1/(-COOH) and 760 cm/sup -1/(msubstituted benzene), Span 60 at 2916 cm/sup -1/(-OH), Span 80 at 1740 cm/sup -1/(5- membered ring), Span 85 at 1643 cm/sup -1/(ketone with 5-membered ring), Tween 20 at 1734 cm/sup 1/ (5-membered ring) and Tween 80 at 3488 cm/sup -1/(-OH). The characteristic peaks of Diacerein were present in niosomal formulations with slight shift at 3355-3379 cm/sup -1/(-COOH) and 760-770 cm/sup -1/(m-substituted benzene). This work suggested no significant interaction in characteristic peaks of Diacerein after combining with nonionic surfactants as physical mixtures and niosomal formulations which proposed potential for niosomes to encapsulate diacerein in their micro vicinity. (author)

  13. Observations of surface-mediated reduction of Pu(VI) to Pu(IV) on hematite nanoparticles by ATR FT-IR

    Energy Technology Data Exchange (ETDEWEB)

    Emerson, Hilary P. [Florida International Univ., Applied Research Center, Miami, FL (United States); Powell, Brian A. [Clemson Univ., Dept. of Enviromental Engineering and Earth Sciences, Anderson, SC (United States)

    2015-07-01

    Previous studies have shown that mineral surfaces may facilitate the reduction of plutonium though the mechanisms of the reduction are still unknown. The objective of this study is to use batch sorption and attenuated total reflectance Fourier transform infrared spectroscopy experiments to observe the surface-mediated reduction of plutonium on hematite nanoparticles. These techniques allow for in situ measurement of reduction of plutonium with time and may lead to a better understanding of the mechanisms of surface mediated reduction of plutonium. For the first time, ATR FT-IR peaks for Pu(VI) sorbed to hematite are measured at ∝ 916 cm{sup -1}, respectively. The decrease in peak intensity with time provides a real-time, direct measurement of Pu(VI) reduction on the hematite surface. In this work pseudo first order rate constants estimated at the high loadings (22 mg{sub Pu}/g{sub hematite}, 1.34 x 10{sup -6} M{sub Pu}/m{sup 2}) for ATR FT-IR are approximately 10 x slower than at trace concentrations based on previous work. It is proposed that the reduced rate constant at higher Pu loadings occurs after the reduction capacity due to trace Fe(II) has been exhausted and is dependent on the oxidation of water and possibly electron shuttling based on the semiconducting nature of hematite. Therefore, the reduction rate at higher loadings is possibly due to the thermodynamic favorability of Pu(IV)-hydroxide complexes.

  14. SUV2, which encodes an ATR-related cell cycle checkpoint and putative plant ATRIP, is required for aluminium-dependent root growth inhibition in Arabidopsis.

    Science.gov (United States)

    Sjogren, Caroline A; Larsen, Paul B

    2017-09-01

    A suppressor mutagenesis screen was conducted in order to identify second site mutations that could reverse the extreme hypersensitivity to aluminium (Al) seen for the Arabidopsis mutant, als3-1. From this screen, it was found that a loss-of-function mutation in the previously described SUV2 (SENSITIVE TO UV 2), which encodes a putative plant ATRIP homologue that is a component of the ATR-dependent cell checkpoint response, reversed the als3-1 phenotype. This included prevention of hallmarks associated with als3-1 including Al-dependent terminal differentiation of the root tip and transition to endoreduplication. From this analysis, SUV2 was determined to be required for halting cell cycle progression and triggering loss of the quiescent centre (QC) following exposure to Al. In conjunction with this, SUV2 was found to have a similar role as ATR, ALT2 and SOG1 in Al-dependent stoppage of root growth, all of which are required for promotion of expression of a suite of genes that likely are part of an Al-dependent DNA damage transcriptional response. This work argues that these Al response factors work together to detect Al-dependent damage and subsequently activate a DNA damage response pathway that halts the cell cycle and subsequently promotes QC differentiation and entrance into endocycling. © 2017 John Wiley & Sons Ltd.

  15. Preparation of Atomically Flat Si(111)-H Surfaces in Aqueous Ammonium Fluoride Solutions Investigated by Using Electrochemical, In Situ EC-STM and ATR-FTIR Spectroscopic Methods

    International Nuclear Information System (INIS)

    Bae, Sang Eun; Oh, Mi Kyung; Min, Nam Ki; Paek, Se Hwan; Hong, Suk In; Lee, Chi-Woo J.

    2004-01-01

    Electrochemical, in situ electrochemical scanning tunneling microscope (EC-STM), and attenuated total reflectance-FTIR (ATR-FTIR) spectroscopic methods were employed to investigate the preparation of atomically flat Si(111)-H surface in ammonium fluoride solutions. Electrochemical properties of atomically flat Si(111)-H surface were characterized by anodic oxidation and cathodic hydrogen evolution with the open circuit potential (OCP) of ca. .0.4 V in concentrated ammonium fluoride solutions. As soon as the natural oxide-covered Si(111) electrode was immersed in fluoride solutions, OCP quickly shifted to near .1 V, which was more negative than the flat band potential of silicon surface, indicating that the surface silicon oxide had to be dissolved into the solution. OCP changed to become less negative as the oxide layer was being removed from the silicon surface. In situ EC-STM data showed that the surface was changed from the initial oxide covered silicon to atomically rough hydrogen-terminated surface and then to atomically flat hydrogen terminated surface as the OCP moved toward less negative potentials. The atomically flat Si(111)-H structure was confirmed by in situ EC-STM and ATR-FTIR data. The dependence of atomically flat Si(111)-H terrace on mis-cut angle was investigated by STM, and the results agreed with those anticipated by calculation. Further, the stability of Si(111)-H was checked by STM in ambient laboratory conditions

  16. Sensing cocaine in saliva with attenuated total reflection infrared (ATR-IR) spectroscopy combined with a one-step extraction method

    Science.gov (United States)

    Hans, Kerstin M.-C.; Gianella, Michele; Sigrist, Markus W.

    2012-03-01

    On-site drug tests have gained importance, e.g., for protecting the society from impaired drivers. Since today's drug tests are majorly only positive/negative, there is a great need for a reliable, portable and preferentially quantitative drug test. In the project IrSens we aim to bridge this gap with the development of an optical sensor platform based on infrared spectroscopy and focus on cocaine detection in saliva. We combine a one-step extraction method, a sample drying technique and infrared attenuated total reflection (ATR) spectroscopy. As a first step we have developed an extraction technique that allows us to extract cocaine from saliva to an almost infrared-transparent solvent and to record ATR spectra with a commercially available Fourier Transform-infrared spectrometer. To the best of our knowledge this is the first time that such a simple and easy-to-use one-step extraction method is used to transfer cocaine from saliva into an organic solvent and detect it quantitatively. With this new method we are able to reach a current limit of detection around 10 μg/ml. This new extraction method could also be applied to waste water monitoring and controlling caffeine content in beverages.

  17. ATR-SEIRAS study of CO adsorption and oxidation on Rh modified Au(111-25 nm) film electrodes in 0.1 M H2SO4

    International Nuclear Information System (INIS)

    Xu, Qinqin; Berná, Antonio; Pobelov, Ilya V.; Rodes, Antonio; Feliu, Juan M.; Wandlowski, Thomas; Kuzume, Akiyoshi

    2015-01-01

    Rh modified Au(111-25 nm) electrodes, prepared by electron beam evaporation and galvanostatic deposition, were employed to study adsorption and electro-oxidation of CO on Rh in 0.1 M sulfuric acid solution by in situ attenuated total reflection surface enhanced infrared absorption spectroscopy (ATR-SEIRAS). The results of ATR-SEIRAS experiments were compared with those obtained by infrared reflection absorption spectroscopy on three low-index Rh single crystal surfaces. The Rh film deposited on Au(111-25 nm) electrode consists of 3D clusters forming a highly stepped [n(111) × (111)]-like surface with narrow (111) terraces. When CO was dosed at the hydrogen adsorption potential region, CO adsorbed in both atop (CO L ) and bridge (CO B ) configurations, as well as coadsorbed water species, were detected on the Rh film electrode. A partial interconversion of spectroscopic bands due to the CO displacement from bridge to atop sites was found during the anodic potential scan, revealing that there is a potential-dependent preference of CO adsorption sites on Rh surfaces. Our data indicate that CO oxidation on Rh electrode surface in acidic media involves coadsorbed water and follows the nucleation and growth model of a Langmuir-Hinshelwood type reaction

  18. Structural dependence of the efficiency of functionalization of silica-coated FeO{sub x} magnetic nanoparticles studied by ATR-IR

    Energy Technology Data Exchange (ETDEWEB)

    Vargas, Angelo; Shnitko, Ivan [Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zurich, Wolfgang Pauli Strasse 10, Hoenggerberg, HCI, 8093 Zurich (Switzerland); Teleki, Alexandra [Institute of Process Engineering, Department of Mechanical and Process Engineering, ETH Zurich, 8092 Zurich (Switzerland); Weyeneth, Stephen [Physik-Institut der Universitaet Zuerich, Winterthurerstrasse 190, 8057 Zuerich (Switzerland); Pratsinis, Sotiris E. [Institute of Process Engineering, Department of Mechanical and Process Engineering, ETH Zurich, 8092 Zurich (Switzerland); Baiker, Alfons, E-mail: baiker@chem.ethz.ch [Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zurich, Wolfgang Pauli Strasse 10, Hoenggerberg, HCI, 8093 Zurich (Switzerland)

    2011-01-15

    The efficiency of propylamino functionalization of magnetic silica-coated FeO{sub x} nanoparticles prepared by different methods, including coprecipitation and flame aerosol synthesis, has been evaluated by attenuated total reflection infrared spectroscopy (ATR-IR) combined with a specific surface reaction, thus revealing the availability of the grafted functional groups. Large differences in the population of reactive groups were observed for the investigated materials, underlining the tight relation between the structure of nanoparticles and their suitability for organic functionalization. The materials possessed different core structure, surface area, and porosity, as evidenced by transmission electron microscopy and nitrogen adsorption-desorption isotherms. Grafting of aminopropyl groups using a standard procedure based on reaction with (3-aminopropyl)trimethoxysilane as source of the propylamino groups was performed, followed by classical dry analysis methods to determine the specific concentration of the organic functional groups (in mmol g{sup -1} of material). ATR-IR spectroscopy in a specially constructed reactor cell was applied as wet methodology to determine the chemically available amount of such functional groups, showing that the materials possess largely different loading capacity, with a variability of up to 70% in the chemical availability of the organic functional group. The amount of (3-aminopropyl)trimethoxysilane used for functionalization was optimized, thus reaching a saturation limit characteristic of the material.

  19. Structural dependence of the efficiency of functionalization of silica-coated FeOx magnetic nanoparticles studied by ATR-IR

    International Nuclear Information System (INIS)

    Vargas, Angelo; Shnitko, Ivan; Teleki, Alexandra; Weyeneth, Stephen; Pratsinis, Sotiris E.; Baiker, Alfons

    2011-01-01

    The efficiency of propylamino functionalization of magnetic silica-coated FeO x nanoparticles prepared by different methods, including coprecipitation and flame aerosol synthesis, has been evaluated by attenuated total reflection infrared spectroscopy (ATR-IR) combined with a specific surface reaction, thus revealing the availability of the grafted functional groups. Large differences in the population of reactive groups were observed for the investigated materials, underlining the tight relation between the structure of nanoparticles and their suitability for organic functionalization. The materials possessed different core structure, surface area, and porosity, as evidenced by transmission electron microscopy and nitrogen adsorption-desorption isotherms. Grafting of aminopropyl groups using a standard procedure based on reaction with (3-aminopropyl)trimethoxysilane as source of the propylamino groups was performed, followed by classical dry analysis methods to determine the specific concentration of the organic functional groups (in mmol g -1 of material). ATR-IR spectroscopy in a specially constructed reactor cell was applied as wet methodology to determine the chemically available amount of such functional groups, showing that the materials possess largely different loading capacity, with a variability of up to 70% in the chemical availability of the organic functional group. The amount of (3-aminopropyl)trimethoxysilane used for functionalization was optimized, thus reaching a saturation limit characteristic of the material.

  20. Chemical transitions of Areca semen during the thermal processing revealed by temperature-resolved ATR-FTIR spectroscopy and two-dimensional correlation analysis

    Science.gov (United States)

    Wang, Zhibiao; Wang, Xu; Pei, Wenxuan; Li, Sen; Sun, Suqin; Zhou, Qun; Chen, Jianbo

    2018-03-01

    Areca semen is a common herb used in traditional Chinese medicine, but alkaloids in this herb are categorized as Group I carcinogens by IARC. It has been proven that the stir-baking process can reduce alkaloids in Areca semen while keep the activity for promoting digestion. However, the changes of compositions other than alkaloids during the thermal processing are unclear. Understanding the thermal chemical transitions of Areca semen is necessary to explore the processing mechanisms and optimize the procedures. In this research, FTIR spectroscopy with a temperature-controlled ATR accessory is employed to study the heating process of Areca semen. Principal component analysis and two-dimensional correlation spectroscopy are used to interpret the spectra to reveal the chemical transitions of Areca semen in different temperature ranges. The loss of a few volatile compounds in the testa and sperm happens below 105 °C, while some esters in the sperm decreases above 105 °C. As the heating temperature is close to 210 °C, Areca semen begins to be scorched and the decomposition of many compounds can be observed. This research shows the potential of the temperature-resolved ATR-FTIR spectroscopy in exploring the chemical transitions of the thermal processing of herbal materials.

  1. Modulation of the DNA repair system and ATR-p53 mediated apoptosis is relevant for tributyltin-induced genotoxic effects in human hepatoma G2 cells.

    Science.gov (United States)

    Li, Bowen; Sun, Lingbin; Cai, Jiali; Wang, Chonggang; Wang, Mengmeng; Qiu, Huiling; Zuo, Zhenghong

    2015-01-01

    The toxic effects of tributyltin (TBT) have been extensively documented in several types of cells, but the molecular mechanisms related to the genotoxic effects of TBT have still not been fully elucidated. Our study showed that exposure of human hepatoma G2 cells to 1-4 μmol/L TBT for 3 hr caused severe DNA damage in a concentration-dependent manner. Moreover, the expression levels of key DNA damage sensor genes such as the replication factor C, proliferating cell nuclear antigen and poly (ADP-ribose) polymerase-1 were inhabited in a concentration-dependent manner. We further demonstrated that TBT induced cell apoptosis via the p53-mediated pathway, which was most likely activated by the ataxia telangiectasia mutated and rad-3 related (ATR) protein kinase. The results also showed that cytochrome c, caspase-3, caspase-8, caspase-9, and the B-cell lymphoma 2 were involved in this process. Taken together, we demonstrated for the first time that the inhibition of the DNA repair system might be more responsible for TBT-induced genotoxic effects in cells. Then the generated DNA damage induced by TBT initiated ATR-p53-mediated apoptosis. Copyright © 2014. Published by Elsevier B.V.

  2. Kaposi sarcoma herpes virus latency associated nuclear antigen protein release the G2/M cell cycle blocks by modulating ATM/ATR mediated checkpoint pathway.

    Directory of Open Access Journals (Sweden)

    Amit Kumar

    Full Text Available The Kaposi's sarcoma-associated herpesvirus infects the human population and maintains latency stage of viral life cycle in a variety of cell types including cells of epithelial, mesenchymal and endothelial origin. The establishment of latent infection by KSHV requires the expression of an unique repertoire of genes among which latency associated nuclear antigen (LANA plays a critical role in the replication of the viral genome. LANA regulates the transcription of a number of viral and cellular genes essential for the survival of the virus in the host cell. The present study demonstrates the disruption of the host G2/M cell cycle checkpoint regulation as an associated function of LANA. DNA profile of LANA expressing human B-cells demonstrated the ability of this nuclear antigen in relieving the drug (Nocodazole induced G2/M checkpoint arrest. Caffeine suppressed nocodazole induced G2/M arrest indicating involvement of the ATM/ATR. Notably, we have also shown the direct interaction of LANA with Chk2, the ATM/ATR signalling effector and is responsible for the release of the G2/M cell cycle block.

  3. A rapid ATR-FTIR spectroscopic method for detection of sibutramine adulteration in tea and coffee based on hierarchical cluster and principal component analyses.

    Science.gov (United States)

    Cebi, Nur; Yilmaz, Mustafa Tahsin; Sagdic, Osman

    2017-08-15

    Sibutramine may be illicitly included in herbal slimming foods and supplements marketed as "100% natural" to enhance weight loss. Considering public health and legal regulations, there is an urgent need for effective, rapid and reliable techniques to detect sibutramine in dietetic herbal foods, teas and dietary supplements. This research comprehensively explored, for the first time, detection of sibutramine in green tea, green coffee and mixed herbal tea using ATR-FTIR spectroscopic technique combined with chemometrics. Hierarchical cluster analysis and PCA principle component analysis techniques were employed in spectral range (2746-2656cm -1 ) for classification and discrimination through Euclidian distance and Ward's algorithm. Unadulterated and adulterated samples were classified and discriminated with respect to their sibutramine contents with perfect accuracy without any false prediction. The results suggest that existence of the active substance could be successfully determined at the levels in the range of 0.375-12mg in totally 1.75g of green tea, green coffee and mixed herbal tea by using FTIR-ATR technique combined with chemometrics. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. PENGUKURAN TINGKAT DAYA SAING DAERAH BERDASARKAN VARIABEL PEREKONOMIAN DAERAH, VARIABEL INFRASTRUKTUR DAN SUMBER DAYA ALAM, SERTA VARIABEL SUMBER DAYA MANUSIA DI WILAYAH PROVINSI SULAWESI TENGGARA

    Directory of Open Access Journals (Sweden)

    Ira Irawati

    2012-10-01

    Full Text Available Daya saing wilayah menunjukkan kemampuan suatu wilayah menciptakan nilai tambah untuk mencapai kesejahteraan yang tinggi dan berkelanjutan dengan tetap terbuka pada persaingan domestik dan  internasional. Pengembangan wilayah di kota-kota dan kabupaten-kabupaten di Provinsi Sulawesi  Tenggara merupakan upaya untuk meningkatkan daya saing tersebut, walaupun  dalam pengembangannya  menghadapi permasalahan-permasalahan yang antara lain disebabkan oleh kurang berkembangnya sumber  daya manusia yang diakibatkan oleh rendahnya tingkat pendidikan dan rendahnya kualitas hidup  masyarakat serta kurangnya prasarana dan sarana untuk menunjang kesejahteraan masyarakat.  Perbandingan relatif tingkat daya saing antar kota kabupaten tersebut; berdasarkan 3 (tiga variabel yaitu  tingkat  perekonomian daerah,  ketersediaan  infrastruktur  dan  sumber  daya  alam,  serta  ketersediaan  dan kualitas sumber daya manusia; dilakukan untuk melihat sejauh mana daerah-daerah tersebut memiliki  keunggulan  untuk  mengatasi  persamalahan-permasalahan  dalam pengembangan wilayahnya. Kata kunci: Pengembangan Wilayah, Daya Saing. Abstract The competitiveness of the region demonstrated the ability of an area to create added value to achieve a high and sustainable prosperity to remain open to domestic and international competition. Development areas in the cities and districts in Southeast Sulawesi is an effort to enhance the competitiveness, even in the face of development issues among others caused by the lack of development of human resources caused by low levels of education and poor quality of life and the lack of infrastructure and facilities to support the welfare of the community. Comparison of the relative level of competitiveness between the city districts; based on 3 (three variables, namely the level of the regional economy, the availability of infrastructure and natural resources, as well as the availability and quality of human resources

  5. PERLINDUNGAN HUKUM TERHADAP PEMEGANG SAHAM DALAM PENJUALAN ASET PERSEROAN BERDASARKAN PASAL 102 AYAT (4 UNDANG-UNDANG NOMOR 40 TAHUN 2007 TENTANG PERSEROAN TERBATAS

    Directory of Open Access Journals (Sweden)

    Musriansyah Musriansyah

    2017-12-01

    Full Text Available Abstract: this study aims to discribe forms of legal protection of shareholders in the company's asset sales in excess of 50% (fifty percent of the amount of the net worth of the company without the approval of the General Meeting Of Shareholders under article 102 paragraph 4 of Act No. 40 of 2007 concerning limited liability companies as well as to know what kind of Board of Directors  responsibility. The study method uses normative juridical with legislation approach. Law No. 40 of 2007 concerning Limited Liability Company provides legal protection by freeing shareholders from personal responsibility for commitments made on behalf of the Company and is not liable for losses of the Company in excess of its shares. Then the responsibility of the board of directors in running its stewardship must be based on in good faith and full of responsibility because the directors hold the fiduciary duty of the company. If the board of directors is negligent in their duties, directors may be subject to piercing the corporate viel of personal liability to personal property of the board of directors for loss received by the company, shareholders or third parties. It is used to protect the interests of the holder or the offending third party for the arbitrary or improper conduct of the board of directors conducted on behalf of the company Abstrak: Kajian ini bertujuan untuk mendeskripsikan bentuk perlindungan hukum terhadap pemegang saham dalam penjualan aset perseroan yang melebihi 50 % (lima puluh persen jumlah kekayaan bersih perseroan tanpa persetujuan RUPS berdasarkan pasal 102 ayat 4 undang-undang nomor 40 tahun 2007 tentang Perseroan Terbatas serta untuk mengetahui bentuk tanggung Jawab direksi terhadap penjualan aset tersebut. Metode kajian menggunakan yuridis normatif dengan pendekatan perundang-undangan. Undang-Undang No.40 Tahun 2007 tentang Perseroan Terbatas memberikan perlindungan hukum dengan membebaskan pemegang saham dari tanggung jawab

  6. A Novel Zn2-Cys6 Transcription Factor AtrR Plays a Key Role in an Azole Resistance Mechanism of Aspergillus fumigatus by Co-regulating cyp51A and cdr1B Expressions

    Science.gov (United States)

    Shimizu, Kiminori; Paul, Sanjoy; Ohba, Ayumi; Gonoi, Tohru; Watanabe, Akira; Gomi, Katsuya

    2017-01-01

    Successful treatment of aspergillosis caused by Aspergillus fumigatus is threatened by an increasing incidence of drug resistance. This situation is further complicated by the finding that strains resistant to azoles, the major antifungal drugs for aspergillosis, have been widely disseminated across the globe. To elucidate mechanisms underlying azole resistance, we identified a novel transcription factor that is required for normal azole resistance in Aspergillus fungi including A. fumigatus, Aspergillus oryzae, and Aspergillus nidulans. This fungal-specific Zn2-Cys6 type transcription factor AtrR was found to regulate expression of the genes related to ergosterol biosynthesis, including cyp51A that encodes a target protein of azoles. The atrR deletion mutant showed impaired growth under hypoxic conditions and attenuation of virulence in murine infection model for aspergillosis. These results were similar to the phenotypes for a mutant strain lacking SrbA that is also a direct regulator for the cyp51A gene. Notably, AtrR was responsible for the expression of cdr1B that encodes an ABC transporter related to azole resistance, whereas SrbA was not involved in the regulation. Chromatin immunoprecipitation assays indicated that AtrR directly bound both the cyp51A and cdr1B promoters. In the clinically isolated itraconazole resistant strain that harbors a mutant Cyp51A (G54E), deletion of the atrR gene resulted in a hypersensitivity to the azole drugs. Together, our results revealed that AtrR plays a pivotal role in a novel azole resistance mechanism by co-regulating the drug target (Cyp51A) and putative drug efflux pump (Cdr1B). PMID:28052140

  7. Preliminary Assessment of ATR-C Capabilities to Provide Integral Benchmark Data for Key Structural/Matrix Materials that May be Used for Nuclear Data Testing and Analytical Methods Validation

    Energy Technology Data Exchange (ETDEWEB)

    John D. Bess

    2009-07-01

    The purpose of this document is to identify some suggested types of experiments that can be performed in the Advanced Test Reactor Critical (ATR-C) facility. A fundamental computational investigation is provided to demonstrate possible integration of experimental activities in the ATR-C with the development of benchmark experiments. Criticality benchmarks performed in the ATR-C could provide integral data for key matrix and structural materials used in nuclear systems. Results would then be utilized in the improvement of nuclear data libraries and as a means for analytical methods validation. It is proposed that experiments consisting of well-characterized quantities of materials be placed in the Northwest flux trap position of the ATR-C. The reactivity worth of the material could be determined and computationally analyzed through comprehensive benchmark activities including uncertainty analyses. Experiments were modeled in the available benchmark model of the ATR using MCNP5 with the ENDF/B-VII.0 cross section library. A single bar (9.5 cm long, 0.5 cm wide, and 121.92 cm high) of each material could provide sufficient reactivity difference in the core geometry for computational modeling and analysis. However, to provide increased opportunity for the validation of computational models, additional bars of material placed in the flux trap would increase the effective reactivity up to a limit of 1$ insertion. For simplicity in assembly manufacture, approximately four bars of material could provide a means for additional experimental benchmark configurations, except in the case of strong neutron absorbers and many materials providing positive reactivity. Future tasks include the cost analysis and development of the experimental assemblies, including means for the characterization of the neutron flux and spectral indices. Oscillation techniques may also serve to provide additional means for experimentation and validation of computational methods and acquisition of

  8. Interaction of Zn(II) with hematite nanoparticles and microparticles: Part 2. ATR-FTIR and EXAFS study of the aqueous Zn(II)/oxalate/hematite ternary system.

    Science.gov (United States)

    Ha, Juyoung; Trainor, Thomas P; Farges, François; Brown, Gordon E

    2009-05-19

    Sorption of Zn(II) to hematite nanoparticles (HN) (av diam=10.5 nm) and microparticles (HM) (av diam=550 nm) was studied in the presence of oxalate anions (Ox2-(aq)) in aqueous solutions as a function of total Zn(II)(aq) to total Ox2-(aq) concentration ratio (R=[Zn(II)(aq)]tot/[Ox2-(aq)]tot) at pH 5.5. Zn(II) uptake is similar in extent for both the Zn(II)/Ox/HN and Zn(II)/Ox/HM ternary systems and the Zn(II)/HN binary system at [Zn(II)(aq)](tot)system than for the Zn(II)/Ox/HM ternary and the Zn(II)/HN and Zn(II)/HM binary systems at [Zn(II)(aq)]tot>4 mM. In contrast, Zn(II) uptake for the Zn(II)/HM binary system is a factor of 2 greater than that for the Zn(II)/Ox/HM and Zn(II)/Ox/HN ternary systems and the Zn(II)/HN binary system at [Zn(II)(aq)]totternary system at both R values examined (0.16 and 0.68), attenuated total reflectance Fourier transform infrared (ATR-FTIR) results are consistent with the presence of inner-sphere oxalate complexes and outer-sphere ZnOx(aq) complexes, and/or type A ternary complexes. In addition, extended X-ray absorption fine structure (EXAFS) spectroscopic results suggest that type A ternary surface complexes (i.e., >O2-Zn-Ox) are present. In the Zn(II)/Ox/HN ternary system at R=0.15, ATR-FTIR results indicate the presence of inner-sphere oxalate and outer-sphere ZnOx(aq) complexes; the EXAFS results provide no evidence for inner-sphere Zn(II) complexes or type A ternary complexes. In contrast, ATR-FTIR results for the Zn/Ox/HN sample with R = 0.68 are consistent with a ZnOx(s)-like surface precipitate and possibly type B ternary surface complexes (i.e., >O2-Ox-Zn). EXAFS results are also consistent with the presence of ZnOx(s)-like precipitates. We ascribe the observed increase of Zn(II)(aq) uptake in the Zn(II)/Ox/HN ternary system at [Zn(II)(aq)]tot>or=4 mM relative to the Zn(II)/Ox/HM ternary system to formation of a ZnOx(s)-like precipitate at the hematite nanoparticle/water interface.

  9. Quantitative determination of polyphosphate in sediments using Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy and partial least squares regression.

    Science.gov (United States)

    Khoshmanesh, Aazam; Cook, Perran L M; Wood, Bayden R

    2012-08-21

    Phosphorus (P) is a major cause of eutrophication and subsequent loss of water quality in freshwater ecosystems. A major part of the flux of P to eutrophic lake sediments is organically bound or of biogenic origin. Despite the broad relevance of polyphosphate (Poly-P) in bioremediation and P release processes in the environment, its quantification is not yet well developed for sediment samples. Current methods possess significant disadvantages because of the difficulties associated with using a single extractant to extract a specific P compound without altering others. A fast and reliable method to estimate the quantitative contribution of microorganisms to sediment P release processes is needed, especially when an excessive P accumulation in the form of polyphosphate (Poly-P) occurs. Development of novel approaches for application of emerging spectroscopic techniques to complex environmental matrices such as sediments significantly contributes to the speciation models of P mobilization, biogeochemical nutrient cycling and development of nutrient models. In this study, for the first time Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy in combination with partial least squares (PLS) was used to quantify Poly-P in sediments. To reduce the high absorption matrix components in sediments such as silica, a physical extraction method was developed to separate sediment biological materials from abiotic particles. The aim was to achieve optimal separation of the biological materials from sediment abiotic particles with minimum chemical change in the sample matrix prior to ATR-FTIR analysis. Using a calibration set of 60 samples for the PLS prediction models in the Poly-P concentration range of 0-1 mg g(-1) d.w. (dry weight of sediment) (R(2) = 0.984 and root mean square error of prediction RMSEP = 0.041 at Factor-1) Poly-P could be detected at less than 50 μg g(-l) d.w. Using this technique, there is no solvent extraction or chemical

  10. Oxidative stress provokes distinct transcriptional responses in the stress-tolerant atr7 and stress-sensitive loh2 Arabidopsis thaliana mutants as revealed by multi-parallel quantitative real-time PCR analysis of ROS marker and antioxidant genes

    NARCIS (Netherlands)

    Mehterov, Nikolay; Balazadeh, Salma; Hille, Jacques; Toneva, Valentina; Mueller-Roeber, Bernd; Gechev, Tsanko

    2012-01-01

    The Arabidopsis thaliana atr7 mutant is tolerant to oxidative stress induced by paraquat (PQ) or the catalase inhibitor aminotriazole (AT), while its original background loh2 and wild-type plants are sensitive. Both, AT and PQ which stimulate the intracellular formation of H2O2 or superoxide anions,

  11. Non-apoptotic programmed cell death with paraptotic-like features in bleomycin-treated plant cells is suppressed by inhibition of ATM/ATR pathways or NtE2F overexpression

    Czech Academy of Sciences Publication Activity Database

    Smetana, O.; Široký, Jiří; Houlné, G.; Opatrný, Z.; Chabouté, M.-E.

    2012-01-01

    Roč. 63, č. 7 (2012), s. 2631-2644 ISSN 0022-0957 Institutional research plan: CEZ:AV0Z50040702 Keywords : ATM /ATR pathways * cell cycle * double-strand break response Subject RIV: BO - Biophysics Impact factor: 5.242, year: 2012

  12. Tratamiento del maxilar posterior atrófico mediante técnica de reconstrucción tridimensional con elevación de seno y abordaje «en tunel»

    Directory of Open Access Journals (Sweden)

    Andrés Restoy

    2015-01-01

    Conclusiones: El tratamiento del sector posterior maxilar atrófico mediante reconstrucción tridimensional con autoinjerto óseo, elevación sinusal y abordaje por tunelización es una técnica que proporciona resultados predecibles y estables, permitiendo la rehabilitación con coronas sobre implantes dentales de dimensiones adecuadas.

  13. Application of reflectance spectroscopies (FTIR-ATR & FT-NIR) coupled with multivariate methods for robust in vivo detection of begomovirus infection in papaya leaves

    Science.gov (United States)

    Haq, Quazi M. I.; Mabood, Fazal; Naureen, Zakira; Al-Harrasi, Ahmed; Gilani, Sayed A.; Hussain, Javid; Jabeen, Farah; Khan, Ajmal; Al-Sabari, Ruqaya S. M.; Al-khanbashi, Fatema H. S.; Al-Fahdi, Amira A. M.; Al-Zaabi, Ahoud K. A.; Al-Shuraiqi, Fatma A. M.; Al-Bahaisi, Iman M.

    2018-06-01

    Nucleic acid & serology based methods have revolutionized plant disease detection, however, they are not very reliable at asymptomatic stage, especially in case of pathogen with systemic infection, in addition, they need at least 1-2 days for sample harvesting, processing, and analysis. In this study, two reflectance spectroscopies i.e. Near Infrared reflectance spectroscopy (NIR) and Fourier-Transform-Infrared spectroscopy with Attenuated Total Reflection (FT-IR, ATR) coupled with multivariate exploratory methods like Principle Component Analysis (PCA) and Partial least square discriminant analysis (PLS-DA) have been deployed to detect begomovirus infection in papaya leaves. The application of those techniques demonstrates that they are very useful for robust in vivo detection of plant begomovirus infection. These methods are simple, sensitive, reproducible, precise, and do not require any lengthy samples preparation procedures.

  14. Application of Attenuated Total Reflectance-Fourier Transformed Infrared (ATR-FTIR) Spectroscopy To Determine the Chlorogenic Acid Isomer Profile and Antioxidant Capacity of Coffee Beans.

    Science.gov (United States)

    Liang, Ningjian; Lu, Xiaonan; Hu, Yaxi; Kitts, David D

    2016-01-27

    The chlorogenic acid isomer profile and antioxidant activity of both green and roasted coffee beans are reported herein using ATR-FTIR spectroscopy combined with chemometric analyses. High-performance liquid chromatography (HPLC) quantified different chlorogenic acid isomer contents for reference, whereas ORAC, ABTS, and DPPH were used to determine the antioxidant activity of the same coffee bean extracts. FTIR spectral data and reference data of 42 coffee bean samples were processed to build optimized PLSR models, and 18 samples were used for external validation of constructed PLSR models. In total, six PLSR models were constructed for six chlorogenic acid isomers to predict content, with three PLSR models constructed to forecast the free radical scavenging activities, obtained using different chemical assays. In conclusion, FTIR spectroscopy, coupled with PLSR, serves as a reliable, nondestructive, and rapid analytical method to quantify chlorogenic acids and to assess different free radical-scavenging capacities in coffee beans.

  15. Solid-state characterization of triamcinolone acetonide nanosuspensiones by X-ray spectroscopy, ATR Fourier transforms infrared spectroscopy and differential scanning calorimetry analysis

    Directory of Open Access Journals (Sweden)

    Eva García-Millán

    2017-12-01

    Full Text Available The data presented in this article describe the physical state of the triamcinolone acetonide (TA in nanosuspension stabilized with polyvinyl alcohol (PVA and poloxamer 407 (PL. The data were assessed by X-ray spectroscopy, ATR Fourier transforms infrared spectroscopy measurements (FTIR, and Differential scanning calorimetry (DSC analysis. PVA, PL and polymeric mixture (PVA and PL were compared with nanosuspension and the interactions between drug triamcinolone acetonide and polymers were studied. The data are related and are complementary to the research article entitle “Improved release of triamcinolone acetonide from medicated soft contact lenses loaded with drug nanosuspensions” (García-Millán et al., 2017 [1]. Keywords: Triamcinolona acetonide nanosuspensiones, X-ray spectroscopy, FTIR spectroscopy, DSC

  16. 2DCOS and PCMW2D analysis of FT-IR/ATR spectra measured at variable temperatures on-line to a polyurethane polymerization

    Science.gov (United States)

    Schuchardt, Patrick; Unger, Miriam; Siesler, Heinz W.

    2018-01-01

    In the present communication the potential of 2DCOS analysis and the spin-off technique perturbation-correlation moving window 2D (PCMW2D) analysis is illustrated with reference to spectroscopic changes observed in a data set recorded by in-line fiber-coupled FT-IR spectroscopy in the attenuated total reflection (ATR) mode during a polyurethane solution polymerization at different temperatures. In view of the chemical functionalities involved, hydrogen bonding plays an important role in this polymerization reaction. Based on the 2DCOS and PCMW2D analysis, the sequence of hydrogen bonding changes accompanying the progress of polymerization and precipitation of solid polymer can be determined. Complementary to the kinetic data derived from the original variable-temperature spectra in a previous publication the results provide a more detailed picture of the investigated solution polymerization.

  17. Study of Barley Grain Molecular Structure for Ruminants Using DRIFT, FTIR-ATR and Synchrotron Radiation Infrared Microspectroscopy (SR-IMS): A Review

    International Nuclear Information System (INIS)

    Yu Peiqiang

    2012-01-01

    Barley inherent structures are highly associated with nutrient utilization and availability in both humans and animals. Barley has different degradation kinetics compared with other cereal grains. It has a relatively higher degradation rate and extent, which often cause digestive disorder in the rumen. Therefore understanding barley inherent structure at cellular and molecular levels and processing-induced structure changes is important, because we can manipulate barley inherent structures and digestive behaviors. Several molecular spectroscopy techniques can be used to detect barley inherent structures at cellular and molecular levels. This article reviews several applications of the IR molecular spectral bioanalytical techniques - DRIFT, FT/IR-ATR and SR-IMS for barley chemistry, molecular structure and molecular nutrition research

  18. New organic single crystal of (benzylthio)acetic acid: Synthesis, crystal structure, spectroscopic (ATR-FTIR, 1H and 13C NMR) and thermal characterization

    Science.gov (United States)

    Sienkiewicz-Gromiuk, Justyna; Tarasiuk, Bogdan; Mazur, Liliana

    2016-04-01

    (Benzylthio)acetic acid (Hbta) was synthesized with 78% yield from benzyl chloride and thiourea as substrates. Well-shaped crystals of Hbta were grown by slow solvent evaporation technique from pure methanol. The compound was investigated by single-crystal X-ray and powder diffraction techniques and was also characterized by other analytical methods, like ATR-FTIR, 1H and 13C NMR and TG/DSC. The acid molecule adopts bent conformation in the solid state. The crystal structure of Hbta is stabilized by numerous intermolecular interactions, including O-H···O, C-H···O, C-H···S and C-H···π contacts. Thermal decomposition of the obtained material takes place above 150 °C.

  19. Fourier transform infrared-attenuated total reflectance (FTIR-ATR spectroscopy and chemometric techniques for the determination of adulteration in petrodiesel/biodiesel blends

    Directory of Open Access Journals (Sweden)

    Armando Guerrero Peña

    2014-06-01

    Full Text Available We propose an analytical method based on fourier transform infrared-attenuated total reflectance (FTIR-ATR spectroscopy to detect the adulteration of petrodiesel and petrodiesel/palm biodiesel blends with African crude palm oil. The infrared spectral fingerprints from the sample analysis were used to perform principal components analysis (PCA and to construct a prediction model using partial least squares (PLS regression. The PCA results separated the samples into three groups, allowing identification of those subjected to adulteration with palm oil. The obtained model shows a good predictive capacity for determining the concentration of palm oil in petrodiesel/biodiesel blends. Advantages of the proposed method include cost-effectiveness and speed; it is also environmentally friendly.

  20. Evaluation of Corrosion of the Dummy “EE” Plate 19 in YA Type ATR Fuel Element During Reactor PALM Cycles

    Energy Technology Data Exchange (ETDEWEB)

    Brower, Jeffrey Owen [Idaho National Lab. (INL), Idaho Falls, ID (United States); Glazoff, Michael Vasily [Idaho National Lab. (INL), Idaho Falls, ID (United States); Eiden, Thomas John [Idaho National Lab. (INL), Idaho Falls, ID (United States); Rezvoi, Aleksey Victor [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2016-08-01

    Advanced Test Reactor (ATR) Cycle 153B-1 was a 14-day, high-power, powered axial locator mechanism (PALM) operating cycle that completed on April 12, 2013. Cycle 153B-1 was a typical operating cycle for the ATR and did not result in any unusual plant transients. ATR was started up and shut down as scheduled. The PALM drive physically moves the selected experiments into and out of the core to simulate reactor startup and heat up, and shutdown and cooldown transients, while the reactor remains in steady state conditions. However, after the cycle was over, several thousand of the flow-assisted corrosion pits and “horseshoeing” defects were readily observable on the surface of the several YA-type fuel elements (these are “dummy” plates that contain no fuel). In order understand these corrosion phenomena a thermal-hydraulic model of coolant channel 20 on a YA-M fuel element was generated. The boundaries of the model were the aluminum EE plate of a YA-M fuel element and a beryllium reflector block with 13 horizontal saw cuts which represented regions of zero flow. The heat generated in fuel plates 1 through 18 was modeled to be passing through the aluminum EE plate. The coolant channel 20 width was set at 0.058 in. (58 mils). It was established that the horizontal saw cuts had a significant effect on the temperature of the coolant. The flow, which was expected to vary linearly with gradual heating of the coolant as it passed through the channel, was extremely turbulent. The temperature rise, which was expected to be a smooth “S” curve, was represented by a series temperature rise “humps,” which occurred at each horizontal saw cut in the beryllium reflector block. Each of the 13 saw cuts had a chamfered edge which resulted in the coolant flow being re-directed as a jet across the coolant channel into the surface of the EE plate, which explained the temperature rise and the observed sscalloping and possibly pitting degradation on the YA-M fuel elements. In

  1. Application of reflectance spectroscopies (FTIR-ATR & FT-NIR) coupled with multivariate methods for robust in vivo detection of begomovirus infection in papaya leaves.

    Science.gov (United States)

    Haq, Quazi M I; Mabood, Fazal; Naureen, Zakira; Al-Harrasi, Ahmed; Gilani, Sayed A; Hussain, Javid; Jabeen, Farah; Khan, Ajmal; Al-Sabari, Ruqaya S M; Al-Khanbashi, Fatema H S; Al-Fahdi, Amira A M; Al-Zaabi, Ahoud K A; Al-Shuraiqi, Fatma A M; Al-Bahaisi, Iman M

    2018-06-05

    Nucleic acid & serology based methods have revolutionized plant disease detection, however, they are not very reliable at asymptomatic stage, especially in case of pathogen with systemic infection, in addition, they need at least 1-2days for sample harvesting, processing, and analysis. In this study, two reflectance spectroscopies i.e. Near Infrared reflectance spectroscopy (NIR) and Fourier-Transform-Infrared spectroscopy with Attenuated Total Reflection (FT-IR, ATR) coupled with multivariate exploratory methods like Principle Component Analysis (PCA) and Partial least square discriminant analysis (PLS-DA) have been deployed to detect begomovirus infection in papaya leaves. The application of those techniques demonstrates that they are very useful for robust in vivo detection of plant begomovirus infection. These methods are simple, sensitive, reproducible, precise, and do not require any lengthy samples preparation procedures. Copyright © 2018 Elsevier B.V. All rights reserved.

  2. POLA SEBARAN SEDIMEN DASAR BERDASARKAN KARAKTERISTIK MORFOLOGI DAN HIDRO-OSEANOGRAFI MENGGUNAKAN MODEL INTERPOLASI DAN SIMULASI NUMERIK DI PERAIRAN UTARA PULAU SIMEULUECUT

    Directory of Open Access Journals (Sweden)

    Ulung Jantama Wisha

    2017-05-01

    Full Text Available Pulau Simeulue Cut merupakan kawasan Konservasi yang nantinya akan diarahkan untuk Daerah Perlindungan Laut (DPL dan Taman Wisata Bahari (TWB, sehingga diperlukan Managemen Plan Kawasan Konservasi Laut Daerah. Tujuan dari penelitian ini adalah untuk mengetahui sebaran sedimen cross-shore di pesisir Pulau Simeulue cut berdasarkan kajian batimetri dan arus pasang surut. Metode penelitian yang digunakan adalah metode studi kasus untuk mengetahui kedalaman, pola sebaran sedimen permukaan dasar laut dan kondisi oseanografi fisika di perairan Simeuluecut secara kualitatif. Hasil pemeruman dianalisis secara spasial dengan menggunakan software Surfer 10 dan ArcGIS 10, Pengukuran arus dan pasang surut dengan menggunakan alat ADCP dan simulasi numerik hidrodinamika dengan menggunakan software MIKE 21 dan pengolahan data pasang surut dengan metode admiralty. Kedalaman perairan berkisar antara 0 - 26 meter dan kelerengan berkisar antara 10% - 15%, sedimen permukaan dasar diperoleh tiga satuan sedimen yaitu pasir kasar, satuan pasir sedang dan satuan pasir halus lanauan (silty sand, verifikasi hasil permodelan didapatkan RMSE sebesar 11,7 %. Kecepatan arus berkisar antara 0 - 0,02 m.s-1pada saat surut menuju pasang dan berkisar antara 0-0,006 m.s-1 pada saat pasang menuju surut, untuk kecepatan arus longshore berkisar antara 0,006 - 0,027 m.s-1 pada kondisi surut menuju pasang dan berkisar antara 0,001 - 0,006 m.s-1 pada kondisi pasang menuju surut, hasil pasang surut didapatkan nilai MSL sebesar 12,53 meter dan tidal range sebesar 2,2 meter, Kondisi oseanografi mempengaruhi distribusi sedimen cross-shore di bibir pantai dan secara langsung mempengaruhi kondisi batimetri di perairan Simeuluecut.Kata Kunci: Batimetri, hidrodinamika, sedimen, Simeuluecut, spatial analisis DISTRIBUTION PATTERNS OF BOTTOM SEDIMENT BASED FROM MORPHOLOGICAL AND HYDRO-OCEANOGRAPHY CHARACTERISTICS USING INTERPOLATION AND NUMERICAL SIMULATION MODEL IN SIMEULUECUT ISLAND NORTHERN

  3. Preliminary Assessment of ATR-C Capabilities to Provide Integral Benchmark Data for Key Structural/Matrix Materials that May be Used for Nuclear Data Testing and Analytical Methods Validation

    Energy Technology Data Exchange (ETDEWEB)

    John D. Bess

    2009-03-01

    The purpose of this research is to provide a fundamental computational investigation into the possible integration of experimental activities with the Advanced Test Reactor Critical (ATR-C) facility with the development of benchmark experiments. Criticality benchmarks performed in the ATR-C could provide integral data for key matrix and structural materials used in nuclear systems. Results would then be utilized in the improvement of nuclear data libraries and as a means for analytical methods validation. It is proposed that experiments consisting of well-characterized quantities of materials be placed in the Northwest flux trap position of the ATR-C. The reactivity worth of the material could be determined and computationally analyzed through comprehensive benchmark activities including uncertainty analyses. Experiments were modeled in the available benchmark model of the ATR using MCNP5 with the ENDF/B-VII.0 cross section library. A single bar (9.5 cm long, 0.5 cm wide, and 121.92 cm high) of each material could provide sufficient reactivity difference in the core geometry for computational modeling and analysis. However, to provide increased opportunity for the validation of computational models, additional bars of material placed in the flux trap would increase the effective reactivity up to a limit of 1$ insertion. For simplicity in assembly manufacture, approximately four bars of material could provide a means for additional experimental benchmark configurations, except in the case of strong neutron absorbers and many materials providing positive reactivity. Future tasks include the cost analysis and development of the experimental assemblies, including means for the characterization of the neutron flux and spectral indices. Oscillation techniques may also serve to provide additional means for experimentation and validation of computational methods and acquisition of integral data for improving neutron cross sections. Further assessment of oscillation

  4. MRN- and 9-1-1-Independent Activation of the ATR-Chk1 Pathway during the Induction of the Virulence Program in the Phytopathogen Ustilago maydis.

    Directory of Open Access Journals (Sweden)

    María Tenorio-Gómez

    Full Text Available DNA damage response (DDR leads to DNA repair, and depending on the extent of the damage, to further events, including cell death. Evidence suggests that cell differentiation may also be a consequence of the DDR. During the formation of the infective hypha in the phytopathogenic fungus Ustilago maydis, two DDR kinases, Atr1 and Chk1, are required to induce a G2 cell cycle arrest, which in turn is essential to display the virulence program. However, the triggering factor of DDR in this process has remained elusive. In this report we provide data suggesting that no DNA damage is associated with the activation of the DDR during the formation of the infective filament in U. maydis. We have analyzed bulk DNA replication during the formation of the infective filament, and we found no signs of impaired DNA replication. Furthermore, using RPA-GFP fusion as a surrogate marker of the presence of DNA damage, we were unable to detect any sign of DNA damage at the cellular level. In addition, neither MRN nor 9-1-1 complexes, both instrumental to transmit the DNA damage signal, are required for the induction of the above mentioned cell cycle arrest, as well as for virulence. In contrast, we have found that the claspin-like protein Mrc1, which in other systems serves as scaffold for Atr1 and Chk1, was required for both processes. We discuss possible alternative ways to trigger the DDR, independent of DNA damage, in U. maydis during virulence program activation.

  5. In situ observation on the dynamic process of evaporation and crystallization of sodium nitrate droplets on a ZnSe substrate by FTIR-ATR.

    Science.gov (United States)

    Zhang, Qing-Nuan; Zhang, Yun; Cai, Chen; Guo, Yu-Cong; Reid, Jonathan P; Zhang, Yun-Hong

    2014-04-17

    Sodium nitrate is a main component of aging sea salt aerosol, and its phase behavior has been studied repeatedly with wide ranges observed in the efflorescence relative humidity (RH) in particular. Studies of the efflorescence dynamics of NaNO3 droplets deposited on a ZnSe substrate are reported, using an in situ Fourier transform infrared attenuated total reflection (FTIR-ATR) technique. The time-dependence of the infrared spectra of NaNO3 aerosols accompanying step changes in RH have been measured with high signal-to-noise ratio. From the IR difference spectra recorded, changes of the time-dependent absorption peak area of the O-H stretching band (ν-OH, ∼3400 cm(-1)) and the nitrate out-of-plane bending band (ν2-NO3(-), ∼836 cm(-1)) are obtained. From these measurements, changes in the IR signatures can be attributed to crystalline and solution phase nitrate ions, allowing the volume fraction of the solution droplets that have crystallized to be determined. Then, using these clear signatures of the volume fraction of droplets that have yet to crystallize, the homogeneous and heterogeneous nucleation kinetics can be studied from conventional measurements using a steady decline in RH. The nucleation rate measurements confirm that the rate of crystallization in sodium nitrate droplets is considerably less than in ammonium sulfate droplets at any particular degree of solute supersaturation, explaining the wide range of efflorescence RHs observed for sodium nitrate in previous studies. We demonstrate that studying nucleation kinetics using the FTIR-ATR approach has many advantages over brightfield imaging studies on smaller numbers of larger droplets or measurements made on single levitated particles.

  6. Técnicas FT-IR (PAS, UATR e Objetiva ATR Aplicadas à Caracterização de EPDM Modificada com Plasma

    Directory of Open Access Journals (Sweden)

    Renata Patrícia dos Santos

    2014-06-01

    Full Text Available Técnicas FT-IR (UATR, PAS e MIC, com objetiva ATR foram escolhidas para a caracterização da superfície da borracha de EPDM vulcanizada, após tratamento em plasma de Ar/O2 e N2/H2/Ar gerado em micro-ondas. Após tratamento, grupos oxigenados foram detectados por UATR e MIC/FT-IR, com objetiva ATR para as duas misturas gasosas, e possíveis grupos nitrogenados foram inseridos na superfície das amostras tratadas com plasma de N2/H2/Ar. A análise MIC/FT-IR sugeriu a formação de grupos em uma camada mais externa, para as amostras tratadas com plasma de N2/H2/Ar, sendo possível observar a redução na intensidade das bandas da absorção do CH2 e CH3, o que pode estar relacionado ao fato de o nitrogênio ser um gás menos permeável que o oxigênio. Diferentes velocidades, 0,05 cm.s-1 e 0,2 cm.s-1, foram utilizadas na análise PAS para avaliar a superfície após tratamento, e apenas para a velocidade 0,05 cm.s-1 com plasma de Ar/O2 alterações espectroscópicas foram detectadas. A redução na medida de ângulo de contato e o aumento na resistência ao descascamento da colagem indicaram melhoras nas propriedades adesivas da superfície. Falhas de adesão foram observadas entre a interface do filme de adesivo de PU e da borracha de EPDM, e confirmados por UATR/FT-IR.