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Sample records for atr kartini berdasarkan

  1. The current status of Kartini research reactor

    Energy Technology Data Exchange (ETDEWEB)

    Tri Wulan Tjiptono; Syarip

    1998-10-01

    The Kartini reactor reached the first criticality on January 25, 1979. In the first three years, the reactor power is limited up to 50 kW thermal power and on July 1, 1982 has been increased to 100 kW. It has been used as experiments facility by researcher of Atomic Energy National Agency and students of the Universities. Three beam tubes used as experiments facilities, the first, is used as a neutron source for H{sub 2}O-Natural Uranium Subcritical Assembly, the second, is developed for neutron radiography facility and the third, is used for gamma radiography facility. The other facilities are rotary rack and two pneumatic transfer systems, one for delayed neutron counting system and the other for the new Neutron Activation Analysis (NAA) facility. The rotary rack used for isotope production for NAA purpose (for long time irradiation), the delayed neutron counting system used for analysis the Uranium contents of the ores and the new NAA is provided for short live elements analysis. In the last three years the Reactor Division has a joint use program with the Nuclear Component and Engineering Center in research reactor instrumentation and control development. (author)

  2. Safety culture and quality management of Kartini research reactor

    Energy Technology Data Exchange (ETDEWEB)

    Syarip [Yogyakarta Nuclear Research Centre, Yogyakarta (Indonesia); Hauptmanns, Ulrich [Department of Plant Design and Safety, Otto-Von-Guericke-University, Magdeburg (Germany)

    1999-10-01

    The evaluation for assessing the safety culture and quality of safety management of Kartini research reactor is presented. The method is based on the concept of management control of safety (audit) as well as by using the developed method i.e. the questionnaires concerning areas of relevance which have to be answered with value statements. There are seven statements or qualifiers in answering the questions. Since such statements are vague, they are represented by fuzzy numbers. The weaknesses can be identified from the different areas contemplated. The evaluation result show that the quality of safety management of Kartini research reactor is globally rated as 'Average'. The operator behavior in the implementation of 'safety culture' concept is found as a weakness, therefore this area should be improved. (author)

  3. Kartini Research Reactor prospective studies for neutron scattering application

    Energy Technology Data Exchange (ETDEWEB)

    Widarto [Yogyakarta Nuclear Research Center, BATAN (Indonesia)

    1999-10-01

    The Kartini Research Reactor (KRR) is located in Yogyakarta Nuclear Research Center, Yogyakarta - Indonesia. The reactor is operated for 100 kW thermal power used for research, experiments and training of nuclear technology. There are 4 beam ports and 1 column thermal are available at the reactor. Those beam ports have thermal neutron flux around 10{sup 7} n/cm{sup 2}s each other and used for sub critical assembly, neutron radiography studies and Neutron Activation Analysis (NAA). Design of neutron collimator has been done for piercing radial beam port and the calculation result of collimated neutron flux is around 10{sup 9} n/cm{sup 2}s. This paper describes experiment facilities and parameters of the Kartini research reactor, and further more the prospective studies for neutron scattering application. The purpose of this paper is to optimize in utilization of the beam ports facilities and enhance the manpower specialty. The special characteristic of the beam ports and preliminary studies, pre activities regarding with neutron scattering studies for KKR is presented. (author)

  4. The Expert System For Safety Assesment Of Kartini Reactor Operation And Maintenance

    International Nuclear Information System (INIS)

    An expert system for safety assessment of Kartini reactor operation and maintenance based on fuzzy logic method has been made. The expert system is developed from the Fuzzy Expert System Tools (FEST), i.e. by developing the knowledge base and data base files of Kartini research reactor system and operations with an inference engine based on FEST. The knowledge base is represented in the procedural knowledge as heuristic rules or generally known as rule-base in the from of If-then rule. The fuzzy inference process and the conclusion of the rule is done by FEST based on direct chaining method with interactive as well as non-interactive modes. The safety assessment of Kartini reactor based on this method gives more realistic value than the conventional method or binary logic

  5. Kartini's Children: On the Need for Thinking Gender and Education Together on a World Scale

    Science.gov (United States)

    Connell, Raewyn

    2010-01-01

    A world policy agenda for gender equality in education now exists, realising the idea of earlier reformers such as Kartini. This agenda, however, makes assumptions that are strongly contested by research and policy debates in national forums. This essay urges shifting the framework of gender analysis to global scale. It outlines what is involved…

  6. Root cause analysis of swelling problem in Kartini reactor

    International Nuclear Information System (INIS)

    Root cause analysis (RCA) is applied for the swelling problem in the Kartini reactor. The RCA result shows that probable root cause of swelling are as follows: It is probable that the seal on the cover plate in the service pool has deteriorated and allowed water to enter both the thermal column and the space between the aluminium reactor pool liner and the concrete. The water will also saturate the concrete and has the potential to corrode the steel reinforcement close to the surface of the concrete. It is believed that water leakage from the service pool has entered the area behind the aluminium pool liner and has saturated the concrete. It is believed that carbon steel reinforcement close to the inner surface of the reactor block has corroded. The expanding corrosion product (rust) has the forced layer of concrete covering the steel reinforcement and subsequently pushing the aluminium pool liner inwards, causing the swelling. There are two issues: the mechanism for the creation of the swellings or bulges dominated by Iron corrosion, and the potential for corrosion of aluminium dominated by the pH of water in contact with the aluminium. As the evidence for this condition were: the apparent corrosion of steel reinforcement behind aluminium liner and formation of Ca(CO3) on outside of concrete reactor block indicating water saturation of the concrete block. The condition is advancing and will most probably result in a loss of pool liner integrity, this may or may not result in a loss of pool water due to the concrete backing behind the pool liner. Based on the above analysis, the remedial actions that can be considered are as follows: The pool liner would be patched with a welded panel that would allow the features we have observed to keep growing in a harmless way. It is expected that they will reach a finite size as the iron is consumed by corrosion. The area behind the pool liner would be dried out, this should limit further growth of the bulges by improving the

  7. Ageing investigation and upgrading of components/systems of Kartini research reactor

    Energy Technology Data Exchange (ETDEWEB)

    Syarip; Widi Setiawan [Yogyakarta Nuclear Research Centre, Yogyakarta (Indonesia)

    1998-10-01

    Kartini research reactor has been operated in good condition and has demonstrated successful operation for the past 18 years, utilized for: reactor kinetic and control studies, instrumentation tests, neutronic and thermohydraulic studies, routine neutron activation analysis, reactor safety studies, training for research reactor operators and supervisors, and reactor physics experiments. Several components of Kartini reactor use components from the abandoned IRT-2000 Project at Serpong and from Bandung Reactor Centre such as: reactor tank, reactor core, heat exchanger, motor blower for ventilation system, fuel elements, etc. To maintain a good operating performance and also for aging investigation purposes, the component failure data collection has been done. The method used is based on the Manual on Reliability Data Collection For Research Reactor PSAs, IAEA TECDOC 636, and analyzed by using Data Entry System (DES) computer code. Analysis result shows that the components/systems failure rate of Kartini reactor is around 1,5.10{sup -4} up to 2,8.10{sup -4} per hour, these values are within the ranges of the values indicated in IAEA TECDOC 478. Whereas from the analysis of irradiation history shows that the neutron fluence of fuel element with highest burn-up (2,05 gram U-235 in average) is around 1.04.10{sup 16} n Cm{sup -2} and this value is still far below its limiting value. Some reactor components/systems have been replaced and upgraded such as heat exchanger, instrumentation and control system (ICS), etc. The new reactor ICS was installed in 1994 which is designed as a distributed structure by using microprocessor based systems and bus system technology. The characteristic and operating performance of the new reactor ICS, as well as the operation history and improvement of the Kartini research reactor is presented. (J.P.N.)

  8. Safety Management and Safety Culture Self Assessment of Kartini Research Reactor

    International Nuclear Information System (INIS)

    The self-assessment of safety culture and safety management status of Kartini research reactor is a step to foster safety culture and management by identifying good practices and areas for improvement, and also to improve reactor safety in a whole. The method used in this assessment is based on questionnaires provided by the Forum for Nuclear Cooperation in Asia (FNCA), then reviewed by experts. Based on the assessment and evaluation results, it can be concluded that there were several good practices in maintaining the safety status of Kartini reactor such as: reactor operators and radiation protection workers were aware and knowledgeable of the safety standards and policies that apply to their operation, readily accept constructive criticism from their management and from the inspectors of regulatory body that address safety performance. As a proof, for the last four years the number of inspection/audit findings from Regulatory Body (BAPETEN) tended to decrease while the reactor utilization and its operating hour increased. On the other hands there were also some comments and recommendations for improvement of reactor safety culture, such as that there should be more frequent open dialogues between employees and managers, to grow and attain a mutual support to achieve safety goals. (author)

  9. Ageing Management Programme: An Experience of In-Service Inspection of the Kartini Heat Exchanger

    International Nuclear Information System (INIS)

    This paper discusses an experience on ISI of the Kartini reactor heat exchanger, as part of the implementation of an ageing management programme. Kartini reactor is located in Yogyakarta, Indonesia. The heat exchanger was constructed for 250 kW capacity. The type of heat exchanger is shell with tube recirculation. Tube material is stainless steel 304, and the shell and baffle plate materials are carbon steel. The heat exchanger has 72 tubes, its outer and inner diameters respectively are 19 and 16 mm, and tube thickness is 1.5 mm. The aim of ISI was to obtain and evaluate the heat exchanger’s condition including the detection of any possible local tube thinning, pitting corrosion or gradual thinning, and determine whether any degradation or deterioration of the heat exchanger could have a significant impact to safety. The heat exchanger was inspected utilizing eddy current equipment in 2003 and 2006. Inspection results in 2003 determined that 12 heat exchanger tubes have a thinning degradation level ranging from 10% up to 60% of the outer diameter due to pitting corrosion. Deterioration of baffle plates has been linked to general corrosion attack. Inspection results in 2006 showed a consistent thinning degradation level with the previous inspection in 2003. So far heat exchanger performance is still satisfactory, as defined by the transfer of primary heat for a 2°C difference between inlet and outlet as required. (author)

  10. ATR performance modeling concepts

    Science.gov (United States)

    Ross, Timothy D.; Baker, Hyatt B.; Nolan, Adam R.; McGinnis, Ryan E.; Paulson, Christopher R.

    2016-05-01

    Performance models are needed for automatic target recognition (ATR) development and use. ATRs consume sensor data and produce decisions about the scene observed. ATR performance models (APMs) on the other hand consume operating conditions (OCs) and produce probabilities about what the ATR will produce. APMs are needed for many modeling roles of many kinds of ATRs (each with different sensing modality and exploitation functionality combinations); moreover, there are different approaches to constructing the APMs. Therefore, although many APMs have been developed, there is rarely one that fits a particular need. Clarified APM concepts may allow us to recognize new uses of existing APMs and identify new APM technologies and components that better support coverage of the needed APMs. The concepts begin with thinking of ATRs as mapping OCs of the real scene (including the sensor data) to reports. An APM is then a mapping from explicit quantized OCs (represented with less resolution than the real OCs) and latent OC distributions to report distributions. The roles of APMs can be distinguished by the explicit OCs they consume. APMs used in simulations consume the true state that the ATR is attempting to report. APMs used online with the exploitation consume the sensor signal and derivatives, such as match scores. APMs used in sensor management consume neither of those, but estimate performance from other OCs. This paper will summarize the major building blocks for APMs, including knowledge sources, OC models, look-up tables, analytical and learned mappings, and tools for signal synthesis and exploitation.

  11. TransOther_ATR

    Data.gov (United States)

    Vermont Center for Geographic Information — The TransOther_ATR layer includes points depicting Automatic Traffice Recorder locations and associated traffic count information maintained by VTrans. The data...

  12. Atlantic Test Range (ATR)

    Data.gov (United States)

    Federal Laboratory Consortium — ATR controls fully-instrumented and integrated test ranges that provide full-service support for cradle-to-grave testing. Airspace and surface target areas are used...

  13. Application of fuzzy set theory for safety culture and safety management assessment of Kartini research reactor

    International Nuclear Information System (INIS)

    The safety culture status of nuclear power plant is usually assessed through interview and/or discussions with personnel and management in plant, and an assessment of the pertinent documentation. The approach for safety culture assessment described in IAEA Safety Series, make uses of a questionnaire composed of questions which require 'Yes' or 'No' as an answer. Hence, it is basically a check-list approach which is quite common for safety assessments in industry. Such a procedure ignores the fact that the expert answering the question usually has knowledge which goes far beyond a mere binary answer. Additionally, many situations cannot readily be described in such restricted terms. Therefore, it was developed a checklist consisting of questions which are formulated such that they require more than a simple 'yes' or 'no' as an answer. This allows one to exploit the expert knowledge of the analyst appropriately by asking him to qualify the degree of compliance of each of the topics examined. The method presented has proved useful in assessing the safety culture and quality of safety management of the research reactor. The safety culture status and the quality of safety management of Kartini research reactor is rated as 'average'. The method is also flexible and allows one to add questions to existing areas or to introduce new areas covering related topics

  14. Analysis and measurement of Kartini reactor parameters for application of K0-NAA method

    International Nuclear Information System (INIS)

    The k0-NAA method is an analysis method that widely used by many of the advantages. In the application of k0-NAA method needed values of reactor parameters (f and α), then the purpose of the research would be conducted measurement parameter values f and a. in the Lazy Susan Kartini reactor facilities. The methods used include Cd-ratio and triple bare methods. The parameter value has been measured in three channels in the irradiation facilities Lazy Susan. Differences of measurement results obtained parameter values are significant in each channel irradiation, the value off ranged from 13,713 to 22,128 and the value of a. ranged from -0,060 to 0,068. The results of measurements for the f and α with the Cd-ratio method, providing more stable value than the triple bare method. The value of f and α obtained can be used a database for the application of k0-NAA method for sample analysis in the laboratory NAA - CAST. (author)

  15. Current status, research progress and future plan of Kartini research reactor

    Energy Technology Data Exchange (ETDEWEB)

    Sardjono, Y.; Syarip; Tjiptono, T.W. [Yogyakarta Nuclear Research Center, Batan (Indonesia)

    1999-10-01

    The current status, research progress and future plan of the Kartini Research Reactor (KRR) is presented. The measurements of axial burn-up distributions for each fuel element by gamma scanning techniques, core axial power distribution display, fuel management for safeguards purpose as well as some research progress activities i.e.; utilization of beamport for: neutron radiography, application neutron activation analysis and history record of KRR power operations is also presented. The KRR is 100 kW pool water reactor type which uses natural circulation and provided by: five beamports in which one of them already coupled with natural uranium subcritical assembly, two thermalizing columns in which one of them is prepared for developing Boron Neutron Capture Therapy (BNCT), two rabbit systems utilized for special analysis uranium ore by delayed neutron counting techniques, one center timbre and 40 irradiation rack (lazy susan) for neutron activation analysis. The KRR was constructed as a second research reactor in Indonesia with special purpose for training and education, high safety margin with involve in high negative temperature coefficient which achieved its first criticality on January 25, 1979. The maximum power level on first criticality is 50 kW and since August 1981 up to now is operating 100 kW. Base on the KRR design limit, it is planned to increase the power level up to 250 kW in the future plan. The preliminary activities such as Non Destructive Testing (NDT) for some reactor components especially water tank and thermal column should be done before decided to increase power level. (author)

  16. KONSEP CANTIK PADA IKLAN CETAK MAJALAH KARTINI TAHUN 2014 DAN KORAN KOMPAS TAHUN 1979: ANALISIS SEMIOTIK

    Directory of Open Access Journals (Sweden)

    Dian Natashia

    2015-12-01

    Full Text Available Advertisements, which can be found in daily life, do not just function as the source of the product’s information, but can also create a new connotation about social reality. This research is intended to reveal the form of the connotation about present beauty of Indonesian women that are trying to be conveyed by the advertisers through present advertisements in Kartini magazines.  This research is also revealing elements that are used by the advertisers to attract readers’ attention. To see what are the new connotation that are formed in the present advertisements, they will be compared with advertisements in the older generation. This research is using qualitative descriptive method and the data is coming from the wordings that are printed in those advertisements. The data will be described and analyzed with Discourse of Advertising Theory by Guy Cook and Anchoring Connotation Theory by Roland Barthes, and then the result will be conclude. Through Guy’s theory, this research will reveal all the elements that are used by the producers to build the beauty concept. Through Barthes’ theory, this research will show how the advertisers create new connotation. The result of analysis shows that present beauty concept about ideal body is a beautiful body that are radiated from the outer side of the women’s body and good looking. Beautiful face is a face that looks as young as twenty years old girls. Moreover, the result also shows that producers use current social representative where consumers these days like new, instant products that give maximum result. The result also shows consumers’ doubt over unknown products and women’s fear on fat body and aging.

  17. PUREX new substation ATR

    International Nuclear Information System (INIS)

    This document is the acceptance test report (ATR) for the New PUREX Main and Minisubstations. It covers the factory and vendor acceptance and commissioning test reports. Reports are presented for the Main 5 kV substation building, the building fire system, switchgear, and vacuum breaker; the minisubstation control building and switch gear; commissioning test; electrical system and loads inspection; electrical utilities transformer and cable; and relay setting changes based on operational experience

  18. PUREX new substation ATR

    Energy Technology Data Exchange (ETDEWEB)

    Nelson, D.E.

    1997-05-12

    This document is the acceptance test report (ATR) for the New PUREX Main and Minisubstations. It covers the factory and vendor acceptance and commissioning test reports. Reports are presented for the Main 5 kV substation building, the building fire system, switchgear, and vacuum breaker; the minisubstation control building and switch gear; commissioning test; electrical system and loads inspection; electrical utilities transformer and cable; and relay setting changes based on operational experience.

  19. Modelling collimator of radial beam port Kartini reactor for boron neutron capture therapy

    International Nuclear Information System (INIS)

    One of the cancer therapy methods is BNCT (Boron Neutron Capture Therapy). BNCT utilizes neutron nature by 10B deposited on cancer cells. The superiority of BNCT compared to the radiation therapy is the high level of selectivity since its level is within cell. This study was carried out on collimator modelling in radial beam port of reactor Kartini for BNCT. The modelling was conducted by simulation using software of Monte Carlo N-Particle version 5 (MCNP 5). MCNP5 is a package of the programs for both simulating and calculating the problem of particle transport by following the life cycle of a neutron since its birth from fission reaction, transport on materials, until eventually lost due to the absorption reaction or out from the system. The collimator modelling used materials which varied in size in order to generate the value of each of the parameters in accordance with the recommendation of the IAEA, the epithermal neutron flux (ϕepi) > 1.0 x 109n.cm-2s-1, the ratio between the neutron dose rate fast and epithermal neutron flux (Df/ϕepi) < 2.0 x 10-13 Gy.cm2.n-1, the ratio of gamma dose rate and epithermal neutron flux (Dγ/ϕepi) < 2.0 X10-13 Gy.cm2.n-1, the ratio between the thermal and epithermal neutron flux (ϕTh/ϕepi)< 0.05 and the ratio between the current and flux of the epithermal neutron (J/ϕepi) > 0.7. Based on the results of the optimization of the modeling, the materials and sizes of the collimator construction obtained were 0.75 cm Ni as collimator wall, 22 cm Al as a moderator and 4.5 cm Bi as a gamma shield. The outputs of the radiation beam generated from collimator modeling of the radial beam port were ϕepi = 5.25 x 106 n.cm-2.s-1, Df/ϕepi = 1.17 x 10-13Gy.cm2.n-1, Dγ/ϕepi = 1.70 x 10-12 Gy.cm2.n-1, ϕTh/ϕepi = 1.51 and J/ϕepi = 0.731. Based on this study, the result of the beam radiation coming out of the radial beam port dis not fully meet the criteria recommended by IAEA so need to continue this study to get the criteria of IAEA

  20. PENENTUAN DAERAH RAWAN GIZI BERDASARKAN ANALISIS SPATIAL

    Directory of Open Access Journals (Sweden)

    Noviati Fuada

    2012-11-01

    Full Text Available Latar Belakang : Riset Kesehatan Dasar telah dilakukan di Indonesia (RISKESDAS 2007. Riset telah mengumpulkan data-data yang terdiri dari data kesehatan yang menggambarkan status gizi anak di bawah lima (antrophometri data di seluruh wilayah Indonesia. Kenyataanya masih sedikit analisis dengan menggunakan metode GIS, oleh karena itu artikel ini akan dikaji dengan metode spasial. Kajian ini diharapkan dapat memberikan informasi faktual, yang dapat mendukung kebijakan daerah. Tujuan: Mengidentifikasi daerah kabupaten/provinsi rawan status gizi anak balita, Metode: Analisa GIS denganmenggunakan metode spasial (pengelompokan data dan overlay dengan cara union. Data RISKESDAS 2007. Hasil: Wilayah tingkat tinggi potensi rawan gizi bermasalah (bersumber overlay antara peta sebaran status gizi balita dengan peta sebaran KK miskin  adalah; Kota Tasikmalaya, Kab. Tasikmalaya, Cianjur, Garut, Ciamis, Bandung, Subang dan Majalengka. Wilayah tingkat tinggi berpotensi terkena infeksi penyakit (berdasarkan peta sebaran resiko Infeksi Penyakit dan pemanfaatan posyandu adalah: Kabupaten Purwakarta, Karawang, Bekasi, Bogor, Sukabumi, Tasikmalaya, Kota Tasikmalaya, Bekasi dan Bogor. Wilayah berpotensi rawan gizi kategori tinggi (bersumber pada 4 faktor/peta sebaran meliputi, Kabupaten Cianjur, Garut, Tasikmalaya dan Kota Tasikmalaya. Kasus Gizi bermasalah berdasarkan 3 indeks gabungan menyebar di seluruh wilayah Provinsi Jawa Barat. Wilayah kasus gizi bermasalah kategori tinggi, dan kategori sedang, sebagian besar  terjadi di wilayah Kabupaten. Baik kategori sedang maupun tinggi merupakan wilayah yang berdampingan. Gambaran ini mengarah pada fakta bahwa  masalah gizi cenderung merupakan masalah epidemiologi. Kesimpulan: Terdapat empat wilayah kabupaten status gizi yang paling serius dalam kategori tinggi meliputi, Kabupaten Cianjur, Garut, Tasikmalaya dan Kota Tasikmalaya.  Kata kunci: analisis spasial, status gizi, posyandu, rawan gizi 

  1. HEATR project: ATR algorithm parallelization

    Science.gov (United States)

    Deardorf, Catherine E.

    1998-09-01

    High Performance Computing (HPC) Embedded Application for Target Recognition (HEATR) is a project funded by the High Performance Computing Modernization Office through the Common HPC Software Support Initiative (CHSSI). The goal of CHSSI is to produce portable, parallel, multi-purpose, freely distributable, support software to exploit emerging parallel computing technologies and enable application of scalable HPC's for various critical DoD applications. Specifically, the CHSSI goal for HEATR is to provide portable, parallel versions of several existing ATR detection and classification algorithms to the ATR-user community to achieve near real-time capability. The HEATR project will create parallel versions of existing automatic target recognition (ATR) detection and classification algorithms and generate reusable code that will support porting and software development process for ATR HPC software. The HEATR Team has selected detection/classification algorithms from both the model- based and training-based (template-based) arena in order to consider the parallelization requirements for detection/classification algorithms across ATR technology. This would allow the Team to assess the impact that parallelization would have on detection/classification performance across ATR technology. A field demo is included in this project. Finally, any parallel tools produced to support the project will be refined and returned to the ATR user community along with the parallel ATR algorithms. This paper will review: (1) HPCMP structure as it relates to HEATR, (2) Overall structure of the HEATR project, (3) Preliminary results for the first algorithm Alpha Test, (4) CHSSI requirements for HEATR, and (5) Project management issues and lessons learned.

  2. Comprehensive In-Service Inspection of Kartini Reactor Tank Liner and Fitness for Continued Operation; Comprehensive In-Service Inspection of Kartini Reactor Tank Liner and Fitness for Continued Operation

    International Nuclear Information System (INIS)

    The preliminary comprehensive in-service inspection of the Kartini reactor core and tank liner by using non-destructive testing (NDT) methods to maintain its safe operation, have been done. The type of NDT used were visual examination using under-water camera and magnifier, replication survey using dental putty, hardness test using an Equotip-D indentor, thickness test using ultrasonic probe, and dye penetrant test. The visual examination showed that the surface of the reactor tank liner was in good condition. The hardness readings were considered to be consistent with the original condition of the tank. The slight increase in hardness at the reactor core area, and this is consistent with the neutron fluence experienced, i.e. ∼1015 n/cm2, where high fluence will cause higher increased hardness of the material. Results of an ultrasonic thickness survey showed that, on average, the tank thickness is between 5.0 mm and 6.5 mm, a low 2.1 mm thickness exists at the top of the tank liner in the belt area. But, because the area is a double layer aluminium plat, its decrement in thickness does not influence the reactor safety. The replica and dye penetrant test at the low thickness area and several suspected areas showed that there could be some defect from the original manufacture. Therefore, it can be concluded that the reactor tank liner is still feasible for continued safe operation. (author)

  3. ANALISIS CITRA PERMUKAAN THERMOCHROMIC LIQUID CRYSTAL BERDASARKAN NILAI STATISTIK HUE

    Directory of Open Access Journals (Sweden)

    Risti Suryantari

    2014-05-01

    Full Text Available Tujuan dari penelitian ini adalah untuk mengamati perbedaan citra pada permukaan Thermocromic Liquid Crystal (TLC R25C5W akibat perubahan temperatur. Metode yang digunakan untuk analisis adalah pengolahan citra berbasis morfologi matematika menggunakan perangkat lunak Matlab2013a. Citra asli yang diperoleh dalam bentuk RGB dikonversi menjadi HSV (hue, saturation, value, dengan mengambil komponen hue saja. Proses utama yang digunakan adalah opening dengan struktur elemen (SE line. Berdasarkan analisis visual pada citra akhir hasil pengolahan citra, tampak bahwa terdapat perbedaan setiap citra untuk berbagai temperatur berdasarkan tingkat kecerahannya. Secara kuantitatif perbedaan tersebut dapat dilihat dari nilai statistiknya. Nilai max dan mean citra hue semakin meningkat seiring dengan meningkatnya temperatur.

  4. ANALISIS PELAYANAN KB MANDIRI WANITA USIA SUBUR BERDASARKAN STATUS EKONOMI

    OpenAIRE

    Selma Siahaan; Siti Isfandari; Rini Sasanti Handayani

    2013-01-01

    Latar belakang: Penggunaan metode keluarga berencana (KB) oleh Wanita Usia Subur (WUS) antara lain dipengaruhi oleh faktor sosial ekonomi. Maka, masalah biaya yang harus dibayar oleh WUS untuk memperoleh pelayanan KB perludi pahami guna keberhasilan program KB Nasional. Metode: Studi menggunakan data Riset Kesehatan Dasar (Riskesdas) 2010, bertujuan untuk mengetahui biaya pelayanan KB yang dibayar oleh perempuan usia 10–59 tahun (WUS) yang pernah kawin menurut metode KB berdasarkan status eko...

  5. MODEL KESESUAIAN APLIKASI UNTUK GOVERNMENT BERDASARKAN BUDAYA ORGANISASI

    Directory of Open Access Journals (Sweden)

    Sri Handayaningsih

    2013-04-01

    Full Text Available Pengembangan E-Government pada sebuah Pemda ditopang oleh empat pilar yaitu kebijakan, kerangka peraturan, manajemen perubahan dan kebutuhan masyarakat. Proses bisnis pada dinas di pemda dan kebutuhan masyarakat terhadap dinas, memunculkan aplikasi yang dapat membantu proses bisnis dalam memberikan layanan pada masyarakat dan manajemen dalam internal dinas. Dalam banyak kasus pengembangan aplikasi pada Pemda tidak berdasarkan pada hal-hal seperti  proses bisnis yang dijalankan di dinas, budaya organisasi dan kebutuhan masyarakat sebagai konsumen namun hanya berdasarkan pada kebutuhan personal dan pemberian dari pusat. Hal ini mengakibatkan aplikasi yang dibangun tidak dapat bertahan lama, tidak dapat membantu seluruh kegiatan bisnis utama dinas dan dijalankan tidak sesuai dengan budaya organisasi yang sedang berjalan saat ini di dinas tersebut. Aplikasi yang harus dibangun dan dijalankan adalah 70% untuk kegiatan pelayanan pada masyarakat dan 30% manajemen organisasi. Pembuatan model kesesuai aplikasi pada sebuah dinas berdasarkan pada budaya yang sedang berjalan di dinas. Budaya organisasi yang berjalan saat ini di pemerintah provinsi DIY adalah budaya clan. Dasar dari kesesuaian aplikasi yang berjalan di sebuah dinas adalah rencana strategis jangka menengah (tahun 2009 s.d 2014. Model akan diukur dengan key performance indicator menggunakan acuan pada COBIT 4.1. Harapannya model dapat digunakan pada dinas di Provinsi DIY

  6. Molecular Pathways: Targeting ATR in Cancer Therapy.

    Science.gov (United States)

    Karnitz, Larry M; Zou, Lee

    2015-11-01

    The human ATR gene encodes a kinase that is activated by DNA damage and replication stress as a central transducer of a checkpoint signaling pathway. Once activated, ATR phosphorylates multiple substrates, including the kinase Chk1, to regulate cell-cycle progression, replication fork stability, and DNA repair. These events promote cell survival during replication stress and in cells with DNA damage. Accordingly, there has been the tantalizing possibility that ATR inhibitors would be therapeutically useful, especially if they were more effective in tumor versus normal cells. Indeed, multiple studies have demonstrated that alterations that promote tumorigenesis, such as defects in the ATM-p53 pathway, constitutive oncogene activation, and acquisition of the alternative lengthening of telomeres pathway, render tumor cells sensitive to ATR inhibitor monotherapy and/or increase the synergy between ATR inhibitors and genotoxic chemotherapies. Now, nearly two decades after the discovery of ATR, two highly selective and potent ATR inhibitors, AZD6738 and VX-970, are in early-phase clinical trials either as monotherapies or paired with a variety of genotoxic chemotherapies. These trials will generate important insights into the effects of ATR inhibition in humans and the potential role of inhibiting this kinase in the treatment of human malignancies. PMID:26362996

  7. IDENTIFIKASI FITUR MELODI GENDING LANCARAN BERDASARKAN PENGENALAN POLA NOTASI

    Directory of Open Access Journals (Sweden)

    Arry Maulana Syarif

    2015-10-01

    Full Text Available Komposisi   gending   mempunyai   karakteristik   tersendiri. Terdapat kemiripan dalam rangkaian notasi di antara gending- gending. Penelitian ini bertujuan untuk mengidentifikasi pola notasi gending berdasarkan kemiripan antar-atributnya. Pasangan notasi ditentukan sebagai dasar analisis, dan algoritma apriori digunakan untuk menganalisis pasangan notasi dari sampel gending lancaran. Hasil yang dicapai dapat memetakan  dan  mengidentifikasi,  serta  memberikan  bobot nilai   ideal   untuk   merangkai   pasangan   notasi,   hingga merangkai gatra (1 gatra terdiri dari 4 notasi. Kata Kunci :pola notasi, apriori, gamelan, gending

  8. ANALISIS PELAYANAN KB MANDIRI WANITA USIA SUBUR BERDASARKAN STATUS EKONOMI

    Directory of Open Access Journals (Sweden)

    Selma Siahaan

    2013-08-01

    Full Text Available Latar belakang: Penggunaan metode keluarga berencana (KB oleh Wanita Usia Subur (WUS antara lain dipengaruhi oleh faktor sosial ekonomi. Maka, masalah biaya yang harus dibayar oleh WUS untuk memperoleh pelayanan KB perludi pahami guna keberhasilan program KB Nasional. Metode: Studi menggunakan data Riset Kesehatan Dasar (Riskesdas 2010, bertujuan untuk mengetahui biaya pelayanan KB yang dibayar oleh perempuan usia 10–59 tahun (WUS yang pernah kawin menurut metode KB berdasarkan status ekonomi yang bersangkutan. Analisis data menggunakan analisis deskriptif, analisis bivariat dengan uji statistik chi-square. Hasil: Alat/obat KB disposible terbanyak yang digunakan WUS dari semua golongan status ekonomi adalah metode KB suntik, sedangkan kondom terbanyak digunakan oleh WUS dengan status ekonomi atas banyak. Sekitar 50% WUS dengan status ekonomi (SE rendah membayar biaya pelayanan KB berkisar Rp.10.000–Rp.15.000. Sementara itu WUS dengan SE atas terbanyak membayar sekitar > Rp. 20.000. Terdapat korelasi signifikan antara metode KB dengan biaya dan status ekonomi. Kesimpulan: Metode suntik masih merupakan metode pilihan bagi WUS di Indonesia dan pemilihan metode KB oleh WUS berhubungan dengan status ekonomi mereka. Oleh karena itu promosi kesehatan perlu terus digalakkan agar program KB pemerintah dengan IUD dapat lebih dikenal dan dapat diterima masyarakat. Di samping itu, diperlukan kebijakan khusus (bantuan kepada WUS dengan status ekonomi rendah sehingga IUD dapat diperoleh secara gratis atau setidaknya dengan biaya yang terjangkau.

  9. Optimasi Kualitas Akustik Room to Room Berdasarkan Nilai Transmission Loss

    Directory of Open Access Journals (Sweden)

    Fitri Rachmawati

    2013-09-01

    Full Text Available Kamar tidur merupakan salah satu fasilitas utama yang disediakan pada sebuah hotel. Fasilitas ini membutuhkan kenyamanan dan ruangan yang bersifat privasi dimana privasi berkaitan dengan aspek suara yaitu bising (noise. Tingkat kebisingan pada kamar harus diatur dengan adanya insulasi suara agar tidak mengganggu kenyamanan pengunjung saat beristirahat. Nilai insulasi ini dapat digambarkan dengan transmission loss dimana nilai ini dapat diubah menjadi suatu nilai tunggal yang disebut dengan weighted sound reduction index (Rw, DnTw yang mempunyai penambahan faktor koreksi suara frekuensi rendah (C, Ctr. Pada penelitian ini dilakukan insulasi suara pada kamar tidur hotel di Bandara Juanda dengan meninjau aspek dinding partisi untuk menahan kebisingan antar ruangan kamar tidur. Hotel pada penelitian ini merupakan hotel low budget dengan kamar yang minimalis dan ditujukan untuk awak pesawat atau penumpang pesawat yang sedang transit. Walaupun minimalis, tetap diperlukan insulasi suara pada kamar yang baik sehingga dilakukan optimasi dinding partisi dengan ketentuan nilai DnTw+Ctr > 51 dB. Pengukuran pada penelitian ini dilakukan di 5 titik dengan menggunakan SLM RION dan SOLO pada saat malam hari. Berdasarkan hasil pengukuran, diketahui bahwa dinding partisi existing belum memenuhi standar dengan nilai DnTw+Ctr sebesar 35,8 dB sehingga dilakukan perancangan ulang dinding partisi. Perancangan ini menggunakan material gypsum board dan cement board dengan variasi ketebalan material serta cavity yang berbeda. Hasil perancangan yang terbaik yaitu dinding dari material cement board 12,5 mm dengan spesifikasi double panels pada masing-masing sisi dan lebar cavity 70 mm dimana menghasilkan nilai DnTw+Ctr sebesar 52,9 dB. Dengan hasil tersebut, maka dinding partisi sudah memenuhi standar dan mempunyai kualitas insulasi suara yang baik.

  10. MIR-ATR sensor for process monitoring

    International Nuclear Information System (INIS)

    A mid-infrared attenuated total reflectance (MIR-ATR) sensor has been developed for chemical reaction monitoring. The optical setup of the compact and low-priced sensor consists of an IR emitter as light source, a zinc selenide (ZnSe) ATR prism as boundary to the process, and four thermopile detectors, each equipped with an optical bandpass filter. The practical applicability was tested during esterification of ethanol and formic acid to ethyl formate and water as a model reaction with subsequent distillation. For reference analysis, a Fourier transform mid-infrared (FT-MIR) spectrometer with diamond ATR module was applied. On-line measurements using the MIR-ATR sensor and the FT-MIR spectrometer were performed in a bypass loop. The sensor was calibrated by multiple linear regression in order to link the measured absorbance in the four optical channels to the analyte concentrations. The analytical potential of the MIR-ATR sensor was demonstrated by simultaneous real-time monitoring of all four chemical substances involved in the esterification and distillation process. The temporal courses of the sensor signals are in accordance with the concentration values achieved by the commercial FT-MIR spectrometer. The standard error of prediction for ethanol, formic acid, ethyl formate, and water were 0.38 mol L  −  1, 0.48 mol L  −  1, 0.38 mol L  −  1, and 1.12 mol L  −  1, respectively. A procedure based on MIR spectra is presented to simulate the response characteristics of the sensor if the transmission ranges of the filters are varied. Using this tool analyte specific bandpass filters for a particular chemical reaction can be identified. By exchanging the optical filters, the sensor can be adapted to a wide range of processes in the chemical, pharmaceutical, and beverage industries. (paper)

  11. The distribution of atrazine (ATR) and ATR metabolites in the Wistar rat following gestational/lactational exposures

    Science.gov (United States)

    Gestational/lactational exposure to ATR is reported to alter reproductive/developmental function, yet our understanding of the transfer of ATR and/or its metabolites from the dam to the fetus/offspring is limited. Previously we examined the lactational transfer of CI4-ATR, but sp...

  12. The Design Method for the ATR High Burnup MOX Fuel

    International Nuclear Information System (INIS)

    The Power Reactor and Nuclear Fuel Development Corporation (PNC) has developed the advanced thermal reactor (ATR). PNC is demonstrating MOX fuel utilization in a prototype of ATR, Fugen (165 MWe), in which 638 MOX fuel assemblies have been loaded without a failure since 1979. PNC is developing the high burn-up MOX fuel for the ATR to contribute to MOX fuels for thermal reactors. The statistical design evaluation method that included the MOX fuel rod performance evaluation code 'FEMAXI-ATR' was developed for the ATR high bum-up MOX fuel rod; it was verified that the integrity of the fuel could be maintained over the whole irradiation period

  13. EKUITAS MEREK JASA GERAI BUAH-BUAHAN BERDASARKAN PERSEPSI KONSUMEN

    Directory of Open Access Journals (Sweden)

    Erdiasa Nursaman

    2015-02-01

    Full Text Available The need of fruits and the number of fruit outlets in Jakarta has been increasing, therefore the study on brand equity of fruit outlet services according to consumer’s perception is now required. In detail the purpose of this study is to analyze the demographic characteristics of  fruits outlets consumer in Jakarta, analyzing the factors that affect brand equity of fruits outlets services, analyze the level of contribution of brand equity variables fruits outlets service in Jakarta and analyze the managerial implications to  fruits outlets brand. Structured questionnaires instrument was used for data collection, while Structural Equation Modeling (SEM method was used in data analysis. The study results have shown that demographic profile of respondents who visited fruit outlet were female, aged 31-40 years, have an undergraduate educational background, private employment and married. Some factors which may influence brand equity are brand loyalty and brand awareness. The brand equity of fruit outlets services is influenced by brand loyalty with contribution of 0.71 and brand awareness with contribution of 0.58. Managerial implication that should be done is to increase promotional activities through advertising. Keywords: brand equity, brand awareness, brand association, brand perceived quality, brand loyaltyABSTRAKLatar belakang dilaksanakannya penelitian ini adalah kebutuhan akan buah-buahan yang semakin meningkat dan makin bertambahnya gerai buah-buahan di Jakarta. Oleh karena itu, diperlukan penelitian ekuitas merek gerai buah-buahan berdasarkan persepsi konsumen. Secara terperinci tujuan penelitian ini menganalisis karakteristik demografi konsumen jasa gerai buah-buahan di Jakarta, menganalisis faktor-faktor yang memengaruhi ekuitas merek jasa layanan gerai buah-buahan, menganalisis tingkat kontribusi peubah-peubah ekuitas merek jasa gerai buah-buahan di Jakarta serta menganalisis implikasi manajerial untuk merek gerai buah-buahan yang

  14. Perbandingan Konsumsi Lemak Berdasarkan Tingkat Keparahan Akne Vulgaris pada Siswa SMK Negeri 1 Kota Jambi

    OpenAIRE

    Nisa Sulistia; Nur Indrawati Lipoeto; Sri Lestari

    2015-01-01

    Abstrak Salah satu faktor pencetus akne vulgaris adalah diet tinggi lemak, terutama lemak jenuh. Tujuan penelitian ini adalah untuk mengetahui perbandingan konsumsi lemak berdasarkan tingkat keparahan akne vulgaris menurut kriteria Lehmann. Penelitian ini berupa studi analitik observasional dengan desain cross sectional melalui pemeriksaan statusdermatologikus menurut kriteria Lehmann dan pengambilan data konsumsi menggunakan FFQ (Food Frequency Questionnaire) pada 138 siswa kelas X dan XI SM...

  15. ATR promotes cilia signalling: links to developmental impacts.

    Science.gov (United States)

    Stiff, Tom; Casar Tena, Teresa; O'Driscoll, Mark; Jeggo, Penny A; Philipp, Melanie

    2016-04-15

    Mutations in ATR(ataxia telangiectasia and RAD3-related) cause Seckel syndrome (ATR-SS), a microcephalic primordial dwarfism disorder. Hitherto, the clinical manifestation of ATR deficiency has been attributed to its canonical role in DNA damage response signalling following replication fork stalling/collapse. Here, we show that ATR regulates cilia-dependent signalling in a manner that can be uncoupled from its function during replication. ATR-depleted or patient-derived ATR-SS cells form cilia of slightly reduced length but are dramatically impaired in cilia-dependent signalling functions, including growth factor and Sonic hedgehog signalling. To better understand the developmental impact of ATR loss of function, we also used zebrafish as a model. Zebrafish embryos depleted of Atr resembled ATR-SS morphology, showed a modest but statistically significant reduction in cilia length and other morphological features indicative of cilia dysfunction. Additionally, they displayed defects in left-right asymmetry including ambiguous expression of southpaw, incorrectly looped hearts and randomized localization of internal organs including the pancreas, features typically conferred by cilia dysfunction. Our findings reveal a novel role for ATR in cilia signalling distinct from its canonical function during replication and strengthen emerging links between cilia function and development. PMID:26908596

  16. An add-on cap for ATR-IR spectroscopy studies

    DEFF Research Database (Denmark)

    2014-01-01

    The invention relates to a cap (300B) for an attenuated total reflectance infrared (ATR-IR) spectrometer, the ATR-IR spectrometer comprising an ATR-IR plate (200). The cap (300B) comprises an ATR- IR plate facing cap surface. When the ATR-IR plate facing cap surface is placed on the sample surfac...

  17. IDENTIFIKASI EMOSI BERDASARKAN ACTION UNIT MENGGUNAKAN METODE BÉZIER CURVE

    Directory of Open Access Journals (Sweden)

    Puji Aswari

    2016-02-01

    Full Text Available Ekspresi wajah menjadi bahasa yang universal. Bahkan perubahan ekspresi wajah dapat membantu pengambilan keputusan. Pada tahun 1972, Paul Ekman mengklasifikasikan emosi dasar manusia ke dalam enam jenis: senang, sedih, terkejut, marah, takut, dan jijik. Kemudian Ekman dan Wallace Friesen mengembangkan sebuah alat untuk mengukur pergerakan pada wajah yang disebut Facial Action Coding System (FACS. FACS menentukan ekspresi wajah berdasarkan pergerakan otot wajah, yang diistilahkan Action Unit (AU. Penelitian ini bertujuan untuk mengetahui emosi tertarik yang dialami seseorang berdasarkan AU yang telah ditentukan oleh Paul Ekman dengan cara membandingkan dua buah citra, yaitu citra wajah tanpa ekspresi dan citra wajah berekspresi. Hasil penelitian ini memperoleh sebuah aplikasi yang mampu mengidentifikasi emosi tertarik dengan akurasi sebesar 80%, True Positive Rate 80%, dan True Negative Rate 80%. Dengan adanya penelitian ini diharapkan dapat diketahui karakteristik action unit yang membentuk emosi tertarik, juga memberikan masukan bagi proses evaluasi belajar mengajar mata kuliah pemrograman.

  18. ATM and ATR:Sensing DNA damage

    Institute of Scientific and Technical Information of China (English)

    Jun Yang; Zheng-Ping Xu; Yun Huang; Hope E. Hamrick; Penelope J. Duerksen-Hughes; Ying-Nian Yu

    2004-01-01

    Cellular response to genotoxic stress is a very complex process, and it usually starts with the "sensing" or "detection" of the DNA damage, followed by a series of events that include signal transduction and activation of transcription factors. The activated transcription factors induce expressions of many genes which are involved in cellular functions such as DNA repair, cell cycle arrest, and cell death. There have been extensive studies from multiple disciplines exploring the mechanisms of cellular genotoxic responses, which have resulted in the identification of many cellular components involved in this process, including the mitogen-activated protein kinases (MAPKs) cascade. Although the initial activation of protein kinase cascade is not fully understood,human protein kinases ATM (ataxia-telangiectasia, mutated) and ATR (ATM and Rad3-related) are emerging as potential sensors of DNA damage. Current progresses in ATM/ATR research and related signaling pathways are discussed in this review, in an effort to facilitate a better understanding of genotoxic stress response.

  19. Peningkatan Efektifitas Mesin Blowing Berdasarkan Evaluasi Overall Equipment Effectiveness dan FMEA pada Industri Manufaktur Plastik

    OpenAIRE

    Simorangkir, Hendra

    2015-01-01

    CV. Makmur Palas adalah perusahaan yang bergerak dibidang pembuatan kantong plastik asoy. Perusahaan ini melakukan kegiatan produksi dengan menggunakan beberapa mesin yaitu mesin pencincang, mesin boker, mesin mixer, mesin blowing, mesin potong, dan mesin pon. Kapasitas mesin yang paling kecil adalah mesin blowing. Berdasarkan data historis periode Januari - Desember 2014, mesin blowing mengalami downtime sebesar 885,15 jam dari total jam kerja tersedia 4.144 jam. Hal ini menyebabkan perusaha...

  20. Perancangan Kursi Dan Meja Berdasarkan Antropometri Pada Sekolah Sd Siti Hajar

    OpenAIRE

    Siregar, Ramadhani

    2016-01-01

    Meja dan kursi merupakan fasilitas sekolah yang berpengaruh terhadap sikap duduk siswa saat belajar. Postur tubuh siswa akan bekerja secara alami jika menggunakan meja dan kursi yang ergonomis. Sebaliknya, jika meja dan kursi tidak ergonomis maka akan menimbulkan ketidaknyamanan sehingga menyebabkan keluhan musculoskeletal pada siswa. Berdasarkan pengamatan di Sekolah Dasar Siti Hajar, meja dan kursi yang digunakan memiliki ukuran yang sama mulai dari kelas I sampai dengan kelas VI, sedan...

  1. PERBEDAAN AUDIT REPORT LAG BERDASARKAN KANTOR AKUNTAN PUBLIK, OPINI AUDIT DAN JENIS INDUSTRI

    OpenAIRE

    Lensa, Harini Antika Puspa

    2014-01-01

    Penelitian ini dilakukan pada perusahaan-perusahaan yang terdaftar di Bursa Efek Indonesia tahun 2010 sampai tahun 2012. Berdasarkan kriteria sampel penelitian diperoleh 362 perusahaan. Alat analisis yang digunakan adalah independent sample t-test dan one way anova. Hasil penelitian ini menunjukkan bahwa terdapat perbedaan audit report lag antara perusahaan yang diaudit oleh Kantor Akuntan Publik Big Four dengan audit report lag perusahaan yang diaudit oleh Kantor Akuntan Publik Non Big Fo...

  2. Memori English Irregular Verbs Berdasarkan Frekuensi Pengulangan dan Jenis Kelamin Santri

    Directory of Open Access Journals (Sweden)

    H. Sribagus

    2013-01-01

    Full Text Available Abstract: Retention of English Irregular Verbs in Relation to Repetition Frequency and Sex. The study explores the most effective and preferred repetition frequency to facilitate memorizing English ir­regular verbs (EIV by students of different sexes, 35 male students and 30 female students of an Islamic boarding school in West Nusa Tenggara. The data for this experimental study were collected through VCD that records EIV, tests, questionnaires, and interviews. The results of the two-way ANOVA analysis indicate that male students had a higher degree of EIV memory based on repetition frequency, with two-time repetition as the most preferred frequency. For male students, the higher the frequency, the lower the memory. Abstrak: Memori English Irregular Verbs Berdasarkan Frekuensi Pengulangan dan Jenis Kelamin Santri. Penelitian ini bertujuan untuk menentukan frekuensi pengulangan yang efektif dan disukai untuk meningkatkan memori EIV berdasarkan jenis kelamin. Data penelitian eksperimen ini dikumpulkan melalui video compact disk (VCD yang merekam English Irregular Verb, tes, angket dan wawancara terhadap 35 santri putra dan 30 santri putri di pondok pesantren di Nusa Tenggara Barat. Kemudian data dianalisis dengan Anava-dua Jalur. Ditemukan bahwa ada perbedaan taraf memori EIV berdasarkan frekuensi pengulangan antara santri putra dan santri putriMemori EIV santri putra lebih tinggi daripada santri putri. Frekuensi pengulangan yang paling efektif adalah dua kali. Semakin tinggi frekuensi pengulangan, semakin rendah memori. Urutan pilihan frekuensi santri putra adalah 2-4-6, sedangkan santri putri 2-6-4.

  3. PEMODELAN STRUKTUR BAWAH PERMUKAAN DAERAH SUMBER AIR PANAS SONGGORITI KOTA BATU BERDASARKAN DATA GEOMAGNETIK

    Directory of Open Access Journals (Sweden)

    Dafiqiy Ya’lu Ulin Nuha, Novi Avisena

    2012-05-01

    Full Text Available Telah dilakukan penelitian dengan metode geomagnetik pada tanggal 26 -27 April 2011 di daerah Songgoriti Kota Batu dengan tujuan untuk mengetahui pola Anomali Magnet Total dan struktur geologi bawah permukaan. Setelah dilakukan koreksi data yang meliputi koreksi diurnal dan koreksi IGRF maka didapatkan nilai anomali magnet total serta kontinuasi ke atas dan reduksi ke kutub. Selanjutnya dilakukan interpretasi secara kualitatif dan kuantitatif. Interpretasi kuantitatif dilakukan dengan membaca pola kontur anomali magnet lokal dan reduksi ke kutub, sedangkan interpretasi kualitatif dilakukan dengan membuat penampang 2,5 D pada dua lintasan AB dan CD. Berdasarkan interpretasi kuantitatif pada kontur anomali  magnetik lokal didapatkan variasi nilai anomali antara -800 nT-600 nT dengan anomali tinggi terdapat pada arah timur dan barat daerah penelitian, anomali sedang terletak pada daerah tengah penelitian dan anomali rendah terdapat pada sedikit   daerah   tengah   penelitian.   Daerah   penelitian   didominasi   anomali   magnetik   sedang. Berdasarkan interpretasi kualitatif pada model penampang 2,5 D lintasan AB dan CD, didapatkan tujuh body yaitu batuan tufa, batuan tufa, batuan breksi vulkanik, batuan breksi tufaan, batuan lava, batuan basalt, dan batuan andesit. Berdasarkan sifat fisik dari tiap lapisan batuan, diduga batuan sarang dalam sistem geothermal yang berupa sumber air panas di daerah penelitian adalah batuan breksi vulkanik dengan batuan penutup (cap rock berupa batuan tufa. Kata Kunci : Anomali Magnet, Struktur Geologi, Air Panas.

  4. Towards universal enrichment nanocoating for IR-ATR waveguides.

    Science.gov (United States)

    Giammarco, James; Zdyrko, Bogdan; Petit, Laeticia; Musgraves, J David; Hu, Juejun; Agarwal, Anu; Kimerling, Lionel; Richardson, Kathleen; Luzinov, Igor

    2011-08-28

    Polymer multilayered nanocoating capable of concentrating various chemical substances at IR-ATR waveguide surfaces is described. The coating affinity to an analyte played a pivotal role in sensitivity enhancement of the IR-ATR measurements, since the unmodified waveguide did not show any analyte detection. PMID:21734984

  5. ATR Prohibits Replication Catastrophe by Preventing Global Exhaustion of RPA

    DEFF Research Database (Denmark)

    Toledo Lazaro, Luis Ignacio; Altmeyer, Matthias; Rask, Maj-Britt;

    2013-01-01

    induced breakage of stalled forks even in cells with active ATR. Thus, ATR-mediated suppression of dormant origins shields active forks against irreversible breakage via preventing exhaustion of nuclear RPA. This study elucidates how replicating genomes avoid destabilizing DNA damage. Because cancer cells...

  6. 77 FR 67557 - Special Conditions: ATR-GIE Avions de Transport Regional, Models ATR42-500 and ATR72-212A...

    Science.gov (United States)

    2012-11-13

    ...-engine, turbo- propeller driven. The Model ATR42-500 has a maximum takeoff weight of 41,005 pounds and an... Privacy Act Statement can be found in the Federal Register published on April 11, 2000 (65 FR...

  7. ATR FTIR Mapping of Leather Fiber Panels

    Science.gov (United States)

    Tondi, G.; Grünewald, T.; Petutschnigg, A.; Schnabel, T.

    2015-01-01

    Leather fiber panels are very promising materials for many applications, not only for the easy availability of the constituents but also for their outstanding fi re-retardant properties. These innovative composite panels can be an excellent material for building insulation, and in recent times, the interest of industries in this composite board has considerably arisen. For this reason the discrimination of the components in the leather fiber panels is becoming fundamental in order to ensure their homogeneous properties. A method to characterize the surface of these materials is then required. An ATR FTIR mapping system for the leather fiber panels has been performed with a Perkin-Elmer microscope coupled with a Frontier FTIR spectrometer. The system has successfully allowed transforming the optical image to a chemical one. This technique can be considered as a right tool for routine controls of the surface quality, especially when the leather shavings cannot be optically distinguished.

  8. Safety significance of ATR passive safety response attributes

    International Nuclear Information System (INIS)

    The Advanced Test Reactor (ATR) at the Idaho National Engineering Laboratory was designed with some passive safety response attributes which contribute to the safety of the facility. The three passive safety attributes being evaluated in the paper are: 1) In-core and in-vessel natural convection cooling, 2) a passive heat sink capability of the ATR primary coolant system (PCS) for the transfer of decay power from the uninsulated piping to the confinement, and 3) gravity feed of emergency coolant makeup. The safety significance of the ATR passive safety response attributes is that the reactor can passively respond to most transients, given a reactor scram, to provide adequate decay power removal and a significant time for operator action should the normal active heat removal systems and their backup systems both fail. The ATR Interim Level 1 Probabilistic Risk Assessment (PRA) models and results were used to evaluate the significance to ATR fuel damage frequency (or probability) of the above three passive response attributes. The results of the evaluation indicate that the first attribute is a major safety characteristic of the ATR. The second attribute has a noticeable but only minor safety significance. The third attribute has no significant influence on the ATR firewater injection system (emergency coolant system)

  9. ATR-mediated regulation of nuclear and cellular plasticity.

    Science.gov (United States)

    Kidiyoor, Gururaj Rao; Kumar, Amit; Foiani, Marco

    2016-08-01

    ATR (Ataxia Telangiectasia and Rad3-related) is a member of the Phosphatidylinositol 3-kinase-related kinases (PIKKs) family, amongst six other vertebrate proteins known so far. ATR is indispensable for cell survival and its essential role is in sensing DNA damage and initiating appropriate repair responses. In this review we highlight emerging and recent observations connecting ATR to alternative roles in controlling the nuclear envelope, nucleolus, centrosome and other organelles in response to both internal and external stress conditions. We propose that ATR functions control cell plasticity by sensing structural deformations of different cellular components, including DNA and initiating appropriate repair responses, most of which are yet to be understood completely. PMID:27283761

  10. LWRS ATR Irradiation Testing Readiness Status

    Energy Technology Data Exchange (ETDEWEB)

    Kristine Barrett

    2012-09-01

    The Light Water Reactor Sustainability (LWRS) Program was established by the U.S. Department of Energy Office of Nuclear Energy (DOE-NE) to develop technologies and other solutions that can improve the reliability, sustain the safety, and extend the life of the current reactors. The LWRS Program is divided into four R&D Pathways: (1) Materials Aging and Degradation; (2) Advanced Light Water Reactor Nuclear Fuels; (3) Advanced Instrumentation, Information and Control Systems; and (4) Risk-Informed Safety Margin Characterization. This report describes an irradiation testing readiness analysis in preparation of LWRS experiments for irradiation testing at the Idaho National Laboratory (INL) Advanced Test Reactor (ATR) under Pathway (2). The focus of the Advanced LWR Nuclear Fuels Pathway is to improve the scientific knowledge basis for understanding and predicting fundamental performance of advanced nuclear fuel and cladding in nuclear power plants during both nominal and off-nominal conditions. This information will be applied in the design and development of high-performance, high burn-up fuels with improved safety, cladding integrity, and improved nuclear fuel cycle economics

  11. ATR National Scientific User Facility 2009 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Todd R. Allen; Mitchell K. Meyer; Frances Marshall; Mary Catherine Thelen; Jeff Benson

    2010-11-01

    This report describes activities of the ATR NSUF from FY-2008 through FY-2009 and includes information on partner facilities, calls for proposals, users week and education programs. The report also contains project information on university research projects that were awarded by ATR NSUF in the fiscal years 2008 & 2009. This research is university-proposed researcher under a user facility agreement. All intellectual property from these experiments belongs to the university per the user agreement.

  12. Preliminary Discrimination of Butter Adulteration by ATR-FTIR Spectroscopy

    OpenAIRE

    Lucian Cuibus; Rubén Maggio; Vlad Mureșan; Zorița Diaconeasa; Oana Lelia Pop; Carmen Socaciu

    2015-01-01

    The Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR)  was applied for the discrimination of butter samples adulterated with solid fraction of palm oil. For FTIR fingerprinting of butter samples, with or without controlled additions of palm oil as adulterant was firstly obtained, using a Shimatsu Prestige 21 Spectrophotometer, including a horizontal diamond ATR accessory with reflection in the MIR region (3873-690) cm-1.The spiked butter samples including 0 level...

  13. Sistem Sensor Kualitas Minyak Goreng Berdasarkan pada Pengukuran Kapasitansi dan Panjang Berkas Pembiasan Cahaya

    Directory of Open Access Journals (Sweden)

    Zulfan Syah Putra

    2013-03-01

    Full Text Available Minyak merupakan salah satu kebutuhan pokok manusia. Kualitas suatu minyak sangat penting diketahui, karena setiap minyak dengan jenis yang sama mempunyai kualitas yang berbeda. Oleh karena itu, perlu dibuat sebuah teknologi yang tepat guna dan ekonomis untuk mengetahui kualitas minyak tersebut. Pada tugas akhir ini penulis telah membuat Sistem Sensor kualitas Minyak Berdasarkan Pada Pengukuran Kapasitansi Dan Panjang Berkas Pembiasan Cahaya. Sistem untuk pengukuran kapasitansi bekerja menggunakan astabel multivibrator dengan frekuensi 50,332  Khz. Frekuensi  50,332  khz ini selanjutnya dirubah ke tegangan dengan menggunakan F to V konverter. Rangkaian F to V konverter yang dirancang mampu bekerja dari frekuensi 1khz-100 khz. Data tegangan yang diperoleh dari f to v konverter ini akan di baca oleh mikrokontroller sebagai data ADC 10 bit. Dengan berubahnya frekuensi, maka data ADC akan berubah. Untuk panjang berkas pembiasan cahaya, sistem ini menggunakan webcam yang berbasis komputer untuk menghitung panjang berkas pembiasan cahaya dengan mendapatkan nilai pixel setiap sampel minyak. Berdasarkan pengujian didapatkan bahwa sistem ini dapat membedakan kualitas minyak yang meliputi minyak goreng murni, minyak goreng bekas, oli murni, oli bekas

  14. UJI KUALITAS MINYAK GORENG BERDASARKAN INDEKS BIAS CAHAYA MENGGUNAKAN ALAT REFRAKTOMETER SEDERHANA

    Directory of Open Access Journals (Sweden)

    Dody Rahayu Prasetyo

    2014-05-01

    Full Text Available Telah  dilakukan uji kualitas minyak goreng dengan parameter fisika indeks bias menggunakan alat refraktometer sederhana. Minyak goreng yang digunakan adalah dua jenis minyak goreng nabati yang berbeda yaitu minyak goreng A dan B. Masing-masing minyak goreng dipanaskan dengan menambahkan plastik dan dilakukan sebanyak enam kali variasi massa plastik. Minyak goreng panas tersebut dimasukkan ke dalam refraktometer kemudian ditembakan sinar laser melalui salah satu sisi dan diamati sinar yang keluar dari sisi yang lain. Penentuan nilai indeks bias minyak goreng didasarkan pada hukum Snellius dengan cara mengukur jarak sinar bias terhadap garis normal. Kualitas minyak goreng ditentukan berdasarkan nilai indeks bias. Dari hasil penelitian didapatkan bahwa semakin banyak kandungan plastik di dalam minyak goreng, semakin besar pula indeks bias minyak goreng tersebut. Dapat dipahami, laju sinar di dalam minyak goreng semakin berkurang dengan bertambahnya partikel plastik. Jadi, peran partikel plastik sebagai faktor penghambat laju sinar. Berdasarkan hasil penelitian dapat disimpulkan bahwa minyak goreng yang mempunyai indeks bias besar yang terindikasi mengandung plastik memiliki kualitas buruk. Untuk minyak goreng merk A setelah dicampur plastik 1 gram dan 11 gram masing-masing memiliki indeks bias 1,351 dan 1,443. Untuk minyak goreng merk B setelah dicampur plastik 1 gram dan 11 gram  masing-masing memiliki indeks bias 1,297 dan 1,492.

  15. Pengembangan Ekonomi Lokal di Kawasan Perikanan Berdasarkan Preferensi Masyarakat Nelayan Nambangan Kedung Cowek

    Directory of Open Access Journals (Sweden)

    Rheza Firmantyo Pramono

    2013-09-01

    Full Text Available Wilayah nambangan memiliki prosentase 40% sebagai nelayan menggantungkan dengan kondisi pesisir, mengindikasikan bahwa wilayah nambangan memiliki potensi pesisir yang cukup besar. Namun masih mempunyai beberapa keterbatasan yang perlu diperhatikan seperti SDA, SDM, Pendanaan, Prasarana Sarana, Kelembagaan, dan teknologi di kawasan pesisir Nambangan Kedung Cowek. Tujuan penelitian ini untuk merumuskan arahan pengembangan ekonomi lokal wilayah nambangan berdasarkan preferensi masyarakat. Metode yang digunakan dalam penelitian ini menggunakan metode deskriptif kualitatif. Analisis pertama penentuan faktor kurang berkembangnya ekonomi lokal menggunakan teknik delphi, selanjutnya menentukan preferensi masyarakat dan faktor prioritas menggunakan teknik servqual. Melalui metode servqual ditemukan banyak faktor prioritas yang harus dikembangkan yaitu SDA, SDM , Pendanaan, Prasarana Sarana, Kelembagaan, Teknologi, dan Pemasaran. Selanjutnya menentukan arahan pengembangan ekonomi lokal beradasakan faktor prioritas menggunakan teknik triangulasi berdasarkan tujuh faktor priortas dengan menggabungkan studi literatur, studi kasus wilayah lain dan temuan fakta emipiri di lapangan. Hasil akhir berupa peningkatan prasarana sarana berupa penataan lingkungan sempadan pantai, pengelolahan limbah, peningkatan sumberdaya manusia berupa pelatihan mutu pendidikan dan wawasan ekonomi. Selanjutnya peningkatan upaya pemasaran dengan membangun mina store dan peningkatan fasilitas alat tangkap.

  16. Assessment of COTS IR image simulation tools for ATR development

    Science.gov (United States)

    Seidel, Heiko; Stahl, Christoph; Bjerkeli, Frode; Skaaren-Fystro, Paal

    2005-05-01

    Following the tendency of increased use of imaging sensors in military aircraft, future fighter pilots will need onboard artificial intelligence e.g. ATR for aiding them in image interpretation and target designation. The European Aeronautic Defence and Space Company (EADS) in Germany has developed an advanced method for automatic target recognition (ATR) which is based on adaptive neural networks. This ATR method can assist the crew of military aircraft like the Eurofighter in sensor image monitoring and thereby reduce the workload in the cockpit and increase the mission efficiency. The EADS ATR approach can be adapted for imagery of visual, infrared and SAR sensors because of the training-based classifiers of the ATR method. For the optimal adaptation of these classifiers they have to be trained with appropriate and sufficient image data. The training images must show the target objects from different aspect angles, ranges, environmental conditions, etc. Incomplete training sets lead to a degradation of classifier performance. Additionally, ground truth information i.e. scenario conditions like class type and position of targets is necessary for the optimal adaptation of the ATR method. In Summer 2003, EADS started a cooperation with Kongsberg Defence & Aerospace (KDA) from Norway. The EADS/KDA approach is to provide additional image data sets for training-based ATR through IR image simulation. The joint study aims to investigate the benefits of enhancing incomplete training sets for classifier adaptation by simulated synthetic imagery. EADS/KDA identified the requirements of a commercial-off-the-shelf IR simulation tool capable of delivering appropriate synthetic imagery for ATR development. A market study of available IR simulation tools and suppliers was performed. After that the most promising tool was benchmarked according to several criteria e.g. thermal emission model, sensor model, targets model, non-radiometric image features etc., resulting in a

  17. ANALISA PERHITUNGAN KEBUTUHAN PENERANGAN PADA BANGUNAN RIG RAISIS (OFFSHORE BERDASARKAN CLASS ABS DAN BKI BERBASIS VISUAL BASIC

    Directory of Open Access Journals (Sweden)

    Feri Diana

    2014-02-01

    Full Text Available Rig RAISIS adalah salah satu pengeboran milik P.T Apexindo Pratama Duta Tbk (APEX yang disewa oleh P.T TOTAL untuk memperpanjang kontraknya selama beberapa tahun. Rig RAISIS ini adalah bangunan offshore yang sudah lama berdiri namun perlu direnovasi. Untuk merenovasi diperlukan konsultan bangunan maritim yang salah satunya ditangani oleh P.T Terafulk Megantara Design. Dalam hal ini mereka masih menggunakan perhitungan secara manual. Maka untuk merencanakan penerangan dengan mudah dibuatlah program perhitungan lampu menggunakan program Visual Basic. Program ini dirancang menggunakan beberapa menu perhitungan berdasarkan dua formula, yaitu formula yang sudah lama diterapkan di PT Terafulk Megantara Design dan formula secara teoritis. Langkah menggunakan program ini adalah dengan memasukkan dimensi ruangan, iluminasi, dan luminasi maka akan muncul hasil luas ruangan dan jumlah lampu berdasarkan tipe lampu yang sudah dipilih. Program ini bisa digunakan untuk menghitung jumlah lampu berdasarkan 2 class yaitu ABS dan BKI. Berdasarkan 2 class yang sudah dihitung maka class ABS mempunyai jumlah lampu lebih banyak dibandingkan ruangan yang menggunakan class BKI. Program ini juga lebih cepat digunakan oleh perancang dengan waktu 45 menit dibandingkan menggunakan perhitungan manual yang memakan waktu 1,5 jam saat menghitung Deck Level 31.

  18. 76 FR 47520 - Airworthiness Directives; ATR-GIE Avions de Transport Régional Model ATR42 and ATR72 Airplanes

    Science.gov (United States)

    2011-08-05

    ... experienced in-flight elevator travel limitations with unusual effort being necessary on pitch axis to control... continued.airworthiness@atr.fr ; Internet http://www.aerochain.com . You may review copies of the referenced... has experienced in-flight elevator travel limitations with unusual effort being necessary on...

  19. POLA SEBARAN SPESIES TIKUS HABITAT PASAR BERDASARKAN JENIS KOMODITAS DI PASAR KOTA BANJARNEGARA

    Directory of Open Access Journals (Sweden)

    Dwi Ernawati

    2014-01-01

    Full Text Available ABSTRACTRodents (Order Rodentia is a wild animal from mammals class and are known as nuisance animals in humanlife, especially domestic rats. Domestic rats have habitats close to human life such as housing, fields and markets.The market is a place where there are lots of food. The purpose of this research was describing the distribution ofrat species habitat based on the type of commodity markets in the Banjarnegara City Market. The researchmethod was a survey with cross-sectional approach, the whole population was rats in City Market and samplewas trapped rats in Banjarnegara City Market. A research instrument set mouse traps and pieces ofidentification, technical analysis is used descriptively and presented in narrative form and frequency distributiontable. The results showed that the number of rats caught consists of several species, 28 Rattus tanezumi, 1 R.norvegicus and 4 S. murinus. Based on trap location only R. tanezumi found inside kiosk. The percentage of ratpositive trap based on kiosk higher found in groceries kiosk (35%, while the lowest is spice kiosk, vegetables andfruits (4%. Rattus tanezumi more dominantly found than other species.Key words: rats, commodity, market, distributionABSTRAKTikus (Ordo Rodentia merupakan hewan liar dari golongan mamalia dan dikenal sebagai hewan pengganggudalam kehidupan manusia, terutama tikus domestik. Tikus domestik mempunyai habitat dekat dengan kehidupanmanusia seperti perumahan, sawah dan pasar. Pasar merupakan tempat yang banyak makanan. Tujuanpenelitian ini untuk mendeskripsikan pola sebaran spesies tikus habitat pasar berdasarkan jenis komoditas diPasar Kota Banjarnegara. Metode penelitiannya adalah survei dengan pendekatan cross sectional. Populasiseluruh tikus di Pasar Kota Banjarnegara dengan sampel tikus yang tertangkap. Teknis analisis secara deskriptifdan disajikan dalam bentuk narasi dan tabel distribusi frekuensi. Hasil penelitian menunjukkan jumlah tikusyang tertangkap antara lain

  20. Homeostatic regulation of meiotic DSB formation by ATM/ATR

    Energy Technology Data Exchange (ETDEWEB)

    Cooper, Tim J.; Wardell, Kayleigh; Garcia, Valerie; Neale, Matthew J., E-mail: m.neale@sussex.ac.uk

    2014-11-15

    Ataxia–telangiectasia mutated (ATM) and RAD3-related (ATR) are widely known as being central players in the mitotic DNA damage response (DDR), mounting responses to DNA double-strand breaks (DSBs) and single-stranded DNA (ssDNA) respectively. The DDR signalling cascade couples cell cycle control to damage-sensing and repair processes in order to prevent untimely cell cycle progression while damage still persists [1]. Both ATM/ATR are, however, also emerging as essential factors in the process of meiosis; a specialised cell cycle programme responsible for the formation of haploid gametes via two sequential nuclear divisions. Central to achieving accurate meiotic chromosome segregation is the introduction of numerous DSBs spread across the genome by the evolutionarily conserved enzyme, Spo11. This review seeks to explore and address how cells utilise ATM/ATR pathways to regulate Spo11-DSB formation, establish DSB homeostasis and ensure meiosis is completed unperturbed.

  1. Perbandingan Konsumsi Lemak Berdasarkan Tingkat Keparahan Akne Vulgaris pada Siswa SMK Negeri 1 Kota Jambi

    Directory of Open Access Journals (Sweden)

    Nisa Sulistia

    2015-09-01

    Full Text Available Abstrak Salah satu faktor pencetus akne vulgaris adalah diet tinggi lemak, terutama lemak jenuh. Tujuan penelitian ini adalah untuk mengetahui perbandingan konsumsi lemak berdasarkan tingkat keparahan akne vulgaris menurut kriteria Lehmann. Penelitian ini berupa studi analitik observasional dengan desain cross sectional melalui pemeriksaan statusdermatologikus menurut kriteria Lehmann dan pengambilan data konsumsi menggunakan FFQ (Food Frequency Questionnaire pada 138 siswa kelas X dan XI SMK Negeri 1 Kota Jambi. Hasil penelitian menunjukkan terdapat akne vulgaris derajat ringan (49%, akne vulgaris derajat sedang (42%, dan akne vulgaris derajat berat (9%. Reratakonsumsi lemak total 89,35 ± 17,63 gram. Rerata konsumsi SFA (Saturated Fatty Acid 37,07 ± 9,97 gram. Rerata konsumsi MUFA (Monounsaturated Fatty Acid 15,30 ± 11,79 gram. Rerata konsumsi PUFA (Polyunsaturated Fatty Acid 12,03 ± 9,25 gram.Uji oneway Anova menunjukkan tidak ada perbedaan bermakna rerata konsumsi lemak total (p > 0,05 dan SFA (p > 0,05 berdasarkan tingkat keparahan akne vulgaris. Uji Kruskal-Wallis menunjukkan tidak ada perbedaan bermakna MUFA (p > 0,05, dan PUFA (p > 0,05 berdasarkan tingkat keparahan akne vulgaris. Penelitian ini menunjukkan tidak ada perbedaan konsumsi lemak berdasarkan tingkat keparahan akne vulgaris pada siswa SMKNegeri 1 Kota Jambi.Kata kunci: akne vulgaris, FFQ, lemak total, SFA, MUFA, PUFA Abstract One of the precipited factors is high fat diet, especially saturated fats. The objective of this study was to determine the comparison of fat consumption which is based on the severity of acne vulgaris.This research wasanalytic observational study using cross sectional design, by examining dermatologic status according to Lehmann criteria and  taking consumption record with FFQ (Food Frequency Questionnaire of  138 tenth and eleventh grade student in SMKN 1 Jambi. The result showed that mild acne vulgaris (49%, moderate acne vulgaris (42%, and

  2. PRODUKTIVITAS TENAGA PENGARIT BERDASARKAN MODA PENGANGKUT DI PETERNAKAN SAPI PERAH PONDOK RANGGON, JAKARTA TIMUR

    Directory of Open Access Journals (Sweden)

    Iwan Prihantoro

    2015-06-01

    Full Text Available Penelitian ini bertujuan untuk menganalisis produktivitas tenaga pengarit dan efektivitasnya berdasarkan moda pengangkut yang dipergunakan di peternakan sapi perah Pondok Ranggon, Jakarta Timur. Peternakan sapi perah Pondok Rangon merupakan salah satu peternakan yang masih bertahan di DKI Jakarta yang ketersediaan hijauan pakannya berasal pada padang rumput alam. Penelitian didasarkan pada sumber data primer dan sekunder dengan cara sensus dari total 22 peternak. Hasil penelitian menunjukkan bahwa karakteristik peternak di Pondok Rangon 40,91% telah berumur > 55 tahun dan 45,46% telah memiliki pengalaman >20 tahun. Kapasitas mengarit tertinggi pada umur 38 tahun (395 kg/hari dan moda truck lebih efisien dalam penyediaan hijauan dibanding pick up dan becak motor.

  3. Analisis keragaman manggis (Garcinia mangostana diiradiasi dengan sinar gamma berdasarkan karakteristik morfologi dan anatomi

    Directory of Open Access Journals (Sweden)

    ALFIN WIDIASTUTI

    2010-11-01

    Full Text Available Abstrak. Widiastuti A, Sobir, Suhartanto MR. 2010. Analisis keragaman manggis (Garcinia mangostana diiradiasi dengan sinar gamma berdasarkan karakteristik morfologi dan anatomi. Bioteknologi 7: 85-98. Tujuan penelitian ini adalah meningkatkan keragaman genetik manggis (Garcinia mangostana L. yang diiradiasi dengan sinar gamma dosis 0 Gy, 20 Gy, 25 Gy, 30 Gy, 35 Gy dan 40 Gy. Bahan tanaman yang digunakan adalah biji yang dikumpulkan dari Kampung Cegal, Desa Karacak, Kecamatan Leuwiliang, Kabupaten Bogor, Jawa Barat. Data dihasilkan dari karakteristik morfologi dan anatomi. Hasil penelitian menunjukkan bahwa peningkatan dosis sinar gamma dapat menghambat pertumbuhan benih, sehingga membutuhkan waktu lebih lama untuk tumbuh dan menurunkan viabilitas benih. Secara morfologi, hal itu juga menurunkan tinggi tanaman, diameter batang, ukuran daun, dan jumlah daun. Secara anatomi, kepadatan stomata berkorelasi positif dengan tinggi tanaman dengan nilai korelasi adalah 90% dan 74%. Iradiasi sinar gamma dapat meningkatkan keragaman morfologi hingga 30%. Pemotongan benih setelah iradiasi dapat meningkatkan keragaman dan tingkat kelangsungan hidup manggis.

  4. FTIR-ATR imaging of coated oil capsules

    International Nuclear Information System (INIS)

    Complete text of publication follows. Coated oil capsules are used to deliver new functional food ingredients. In this study spherical capsules were investigated containing a core with fish oil coated with a transparent gelatine layer. The remaining actives are within a hard coating, which is applied on the gelatine capsules. For the microstructural characterization of the coating the following techniques were used: Fourier Transform Infrared (FTIR) microscopy using an Attenuated Total Reflection (ATR) crystal, Light Microscopy (LM), X-ray microtomography (μCT) and Scanning Electron Microscopy (SEM). For FTIR-ATR imaging the oil capsules were embedded in epoxy resin. The embedded sample was trimmed in a microtome using a glass knife for the initial rough trimming. For the final fracture a diamond knife was used to obtain the best possible surface. A Ge-crystal was brought in contact with the surface of interest. LM images were obtained using the visible light camera of the FTIR system. A principal component analysis was performed on the FTIR-ATR imaging showing the spatial distribution of some of the compounds. This study demonstrated that imaging techniques can be used to analyse the microstructure of coated oil capsules. Correlative microscopy of techniques like FTIR-ATR imaging, LM, XRT and SEM-EDX results in useful information about the spatial distribution of the chemical compounds and layer thickness of solid food samples.

  5. HCLK2 is required for activity of the DNA damage response kinase ATR

    DEFF Research Database (Denmark)

    Rendtlew Danielsen, Jannie M; Larsen, Dorthe Helena; Schou, Kenneth Bødtker;

    2008-01-01

    ATR is a protein kinase that orchestrates the cellular response to replication problems and DNA damage. HCLK2 has previously been reported to stabilize ATR and Chk1. Here we provide evidence that human HCLK2 acts at an early step in the ATR signaling pathway and contributes to full-scale activati...

  6. PENELITIAN PENDAHULUAN VARIASI GENETIK MASYARAKAT SOROH PANDE BERDASARKAN PENANDA DNA MIKROSATELIT KROMOSOM Y: MASYARAKAT SOROH PANDE DESA ABIANSEMAL, BADUNG

    Directory of Open Access Journals (Sweden)

    Ni Putu Putri Wulandari

    2015-02-01

    Full Text Available Masyarakat Hindu di Bali dibedakan ke dalam kelompok-kelompok berdasarkan garis keturunan laki-laki yang disebut klan atau di Bali lebih dikenal dengan istilah soroh. Salah satu soroh yang dikenal di Bali adalah soroh Pande. Penelitian ini bertujuan untuk mengetahui variasi genetik masyarakat soroh Pande di Desa Abiansemal, Badung menggunakan empat lokus mikrosatelit kromosom Y, yaitu DYS19, DYS390, DYS393 dan DYS395. Berdasarkan hasil analisis, didapatkan 11 ragam alel dan enam haplotipe yang didominasi oleh haplotipe A (200, 215, 121, 123 dengan frekuensi sebesar 0,65 serta keragaman genetik sebesar 0,32 ± 0,07. Ragam haplotipe yang didapatkan menunjukkan bahwa masyarakat soroh Pande di Desa Abiansemal berasal dari berbagai sumber gen.

  7. Validation of HELIOS for ATR Core Follow Analyses

    Energy Technology Data Exchange (ETDEWEB)

    Bays, Samuel E. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Swain, Emily T. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Crawford, Douglas S. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Nigg, David W. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-03-01

    This work summarizes the validation analyses for the HELIOS code to support core design and safety assurance calculations of the Advanced Test Reactor (ATR). Past and current core safety assurance is performed by the PDQ-7 diffusion code; a state of the art reactor physics simulation tool from the nuclear industry’s earlier days. Over the past twenty years, improvements in computational speed have enabled the use of modern neutron transport methodologies to replace the role of diffusion theory for simulation of complex systems, such as the ATR. More exact methodologies have enabled a paradigm-shift away from highly tuned codes that force compliance with a bounding safety envelope, and towards codes regularly validated against routine measurements. To validate HELIOS, the 16 ATR operational cycles from late-2009 to present were modeled. The computed power distribution was compared against data collected by the ATR’s on-line power surveillance system. It was found that the ATR’s lobe-powers could be determined with ±10% accuracy. Also, the ATR’s cold startup shim configuration for each of these 16 cycles was estimated and compared against the reported critical position from the reactor log-book. HELIOS successfully predicted criticality within the tolerance set by the ATR startup procedure for 13 out of the 16 cycles. This is compared to 12 times for PDQ (without empirical adjustment). These findings, as well as other insights discussed in this report, suggest that HELIOS is highly suited for replacing PDQ for core safety assurance of the ATR. Furthermore, a modern verification and validation framework has been established that allows reactor and fuel performance data to be computed with a known degree of accuracy and stated uncertainty.

  8. Comparison between ATR-IR, Raman, concatenated ATR-IR and Raman spectroscopy for the determination of total antioxidant capacity and total phenolic content of Chinese rice wine.

    Science.gov (United States)

    Wu, Zhengzong; Xu, Enbo; Long, Jie; Pan, Xiaowei; Xu, Xueming; Jin, Zhengyu; Jiao, Aiquan

    2016-03-01

    The application of attenuated total reflectance infrared spectroscopy (ATR-IR), Raman spectroscopy (RS) and combination of ATR-IR and RS for measurements of total antioxidant capacity (TAC) and total phenolic content (TPC) of Chinese rice wine (CRW) were investigated in this study. Synergy interval partial least-squares (SiPLS), support vector machine (SVM) and principal component analysis (PCA) were applied to process the merged data from two individual instruments. It was observed that the performances of models based on the RS spectra were better than those based on the ATR-IR spectra. In addition, SVM models based on the efficient information extracted from ATR-IR and RS spectra were superior to PLS models based on the same information and PLS models based on ATR-IR or RS spectra. The overall results demonstrated that integrating ATR-IR and RS was possible and could improve the prediction accuracy of TAC and TPC in CRWs.

  9. Evaluasi Dimensi Mesin Jahit dan Kursi Operator Berdasarkan Kriteria Antropometri pada Stasiun Penjahitan di Industri Garmen UD.Chaniago.

    OpenAIRE

    Susanto, Eko

    2011-01-01

    Penelitian ini bertujuan untuk mengevaluasi mesin jahit dan kursi operator yang dipakai di stasiun penjahitan di UD.Chaniago berdasarkan perspektif dimensi antropometri pekerja. Untuk mencapai tujuan, maka dilakukan pembandingan dimensi mesin jahit dan kursi operator yang digunakan saat ini dengan dimensi ukuran antropometri pekerja dan mengindentifikasi apakah terdapat kesesuaian atau ketidaksesuaian. Ukuran dimensi antropometri tubuh pekerja dan dimensi mesin jahit dan kursi operator yang d...

  10. KEMAMPUAN MENULIS BAHASA INGGRIS SISWA MELALUI SELF-EDITING AND SELF-CORRECTING BERDASARKAN ANALISIS KESALAHAN GRAMATIKAL DAN KOSAKATA

    OpenAIRE

    Mathelda Obisuru; Widyastuti Purbani

    2016-01-01

    Penelitian ini bertujuan meningkatkan kemampuan menulis bahasa Inggris siswa melalui self-editing and self-correcting berdasarkan analisis kesalahan tata bahasa dan kosakata. Jenis penelitian ini adalah penelitian tindakan kelas. Data dikumpulkan melalui observasi dan dari tes menulis siswa. Penelitian dilakukan secara kolaboratif dalam dua siklus, dan setiap siklus terdiri dari perencanaan, pelaksanaan, observasi, dan refleksi. Setelah dilakukan tindakan self-editing and self-correcting dala...

  11. Struktur vertikal upwelling – downwelling di Samudera Hindia Selatan Jawa hingga Selatan Bali berdasarkan salinitas musiman periode 2004 – 2010

    OpenAIRE

    Restu Wardani; Widodo S. Pranowo; Elis Indrayanti

    2013-01-01

    Salinitas berperan penting untuk mengidentifikasi fenomena upwelling dan downwelling. Upwelling yang terjadi di samudera Hindia Selatan Jawa hingga Selatan Bali dipengaruhi oleh angin musim, ENSO (El Nino Southern Oscillation) dan IOD (Indian Oscillation Dipole Mode). Dalam peneletian ini dikaji pola upwelling dan downwelling berdasarkan distribusi salinitas secara vertikal dan vaeriabilitas musiman dikaji dalam waktu tujuh tahun (2004 – 2010). Data hasil akuisisi argo float digunakan dalam p...

  12. Analisis Deskriptif Soal Geometri dalam Buku Matematika Bilingual untuk Sekolah Menengah Pertama Kelas VIII Berdasarkan Kriteria International Assessment TIMSS 2007

    OpenAIRE

    Etik Rahayu; Hardi Suyitno; Iwan Junaedi

    2012-01-01

    Tujuan penelitian ini adalah untuk mendeskripsikan  domain kognitif dan aspek kognitif (required behavior) soal matematika dalam Buku Matematika Bilingual untuk Sekolah Menengah Pertama (SMP) Kelas VIII berdasarkan kriteria International Assessment TIMSS 2007 dan proporsinya. Metode penelitian yang digunakan adalah deskriptif kualitatif, dengan subjek penelitian adalah soal geometri dalam Buku Matematika Bilingual SMP. yang berjudul “Mathematics for Junior High School Grade VIII 1st Semest...

  13. Integrate knowledge acquisition with target recognition through closed-loop ATR

    Science.gov (United States)

    Yu, Ssu-Hsin; McLaughlin, Pat; Zatezalo, Aleksandar; Hsiao, Kai-yuh; Boskovic, Jovan

    2015-05-01

    Automatic Target Recognition (ATR) algorithm performance is highly dependent on the sensing conditions under which the input data is collected. Open-loop fly-bys often produce poor results due to less than ideal measurement conditions. In addition, ATR algorithms must be extremely complicated to handle the diverse range of inputs with a resulting reduction in overall performance and increase in complexity. Our approach, closed-loop ATR (CL-ATR), focuses on improving the quality of information input to the ATR algorithms by optimizing motion, sensor settings and team (vehicle-vehicle-human) collaboration to dramatically improve classification accuracy. By managing the data collection guided by predicted ATR performance gain, we increase the information content of the data and thus dramatically improve ATR performance with existing ATR algorithms. CL-ATR has two major functions; first, an ATR utility function, which represents the performance sensitivity of ATR produced classification labels as a function of parameters that correlate to vehicle/sensor states. This utility function is developed off-line and is often available from the original ATR study as a confusion matrix, or it can be derived through simulation without direct access to the inner working of the ATR algorithm. The utility function is inserted into our CLATR framework to autonomously control the vehicle/sensor. Second, an on-board planner maps the utility function into vehicle position and sensor collection plans. Because we only require the utility function on-board, we can activate any ATR algorithm onto a unmanned aerial vehicle (UAV) platform no matter how complex. This pairing of ATR performance profiles with vehicle/sensor controls creates a unique and powerful active perception behavior.

  14. Activation of the ATR kinase by the RPA-binding protein ETAA1

    DEFF Research Database (Denmark)

    Haahr, Peter; Hoffmann, Saskia; Tollenaere, Maxim A X;

    2016-01-01

    Activation of the ATR kinase following perturbations to DNA replication relies on a complex mechanism involving ATR recruitment to RPA-coated single-stranded DNA via its binding partner ATRIP and stimulation of ATR kinase activity by TopBP1. Here, we discovered an independent ATR activation pathway...... in vertebrates, mediated by the uncharacterized protein ETAA1 (Ewing's tumour-associated antigen 1). Human ETAA1 accumulates at DNA damage sites via dual RPA-binding motifs and promotes replication fork progression and integrity, ATR signalling and cell survival after genotoxic insults. Mechanistically......, this requires a conserved domain in ETAA1 that potently and directly stimulates ATR kinase activity independently of TopBP1. Simultaneous loss of ETAA1 and TopBP1 gives rise to synthetic lethality characterized by massive genome instability and abrogation of ATR-dependent signalling. Our findings demonstrate...

  15. ANALISIS PENGUNGKAPAN TANGGUNG JAWAB SOSIAL PERBANKAN SYARIAH DI INDONESIA BERDASARKAN ISLAMIC SOCIAL REPORTING INDEKS

    Directory of Open Access Journals (Sweden)

    Khusnul Fauziah

    2013-05-01

    Full Text Available Penelitian ini dilakukan untuk membandingkan pengungkapan tanggungjawab sosial bank syariah di Indonesia berdasarkan pada indeks Islamic Social Reporting (ISR. Obyek dari penelitian ini adalah tujuh bank syariah di Indonesia. Penelitian ini menggunakan laporan tahunan tahun 2011 yang dipublikasikan pada masing-masing bank. Analisis data menggunakan analisis isi. Hasil penelitian menunjukkan skore indek IRS tertinggi adalah Bank Muamalat Indonesia dengan skore 73% dan skore terendah adalah Bank Panin Syariah dengan skore 41%. This research is intended to compare the social responsibility disclosure of Islamic banking in Indonesia based on the Islamic Social Reporting (ISR index. The object of this research is taken from seven Islamic bankings in Indonesia. This research use the data from the annual report which was published in 2011 by the banks. The content analysis is implemented for analyzing the data. The results show that the highest score of social responsibility disclosure is Bank Muamalat Indonesia with the score of 73% and the lowest score is Panin Bank Syariah with the score of 41%.

  16. PENENTUAN WAKTU PERAWATAN UNTUK PENCEGAHANPADA KOMPONEN KRITIS CYCLONE FEED PUMP BERDASARKAN KRITERIA MINIMASI DOWN TIME

    Directory of Open Access Journals (Sweden)

    Siti Nandiroh

    2006-08-01

    Full Text Available Sistem perawatan mesin yang dilakukan di PT. Newmont Nusa Tenggara, selama ini masih bersifat korektif yaitu perawatan setelah terjadi kerusakan. Kerusakan komponen ini biasanya akan ditandai dengan ditemukannya produk yang dihasilkan tidak sedikit mengalami kecacatan. Peranan perawatan terhadap komponen-komponen Cyclone Feed Pump pada Process Departement - PT.Newmont Nusa Tenggara sangat penting artinya untuk mencegah terjadinya kecacatan produk masal dan mencegah terjadinya down time produksi. Dan perawatan yang paling baik digunakan adalah perawatan pencegahan sebelum terjadinya kerusakan (preventive maintenance. Mesin kritis adalah mesin yang mengalami frekwensi kerusakan terbesar dengan total downtime terbesar. Untuk penentuan mesin kritis ini, langkah pertama yang dilakukan adalah dengan mengukur lamanya waktu downtime produksi dari tiap-tiap mesin yang ada. Perhitungan MTTR berdasarkan data downtime, yang sebelumnya juga dilakukan uji kecocokan distribusi dan hasilnya sesuai, Dengan melakukan perhitungan Mean Time To Repair dan Mean Time To Failure dapat diketahui rata-rata waktu berapa lama pompa beroperasi dan berapa lama pompa tersebut dapat dilakukan perbaikan serta dapat diketahui Reliability pada Cyclone Feed Pump 2.0.1. Setelah dilakukkan perhitungan, komponen kritis Discharge Pipe pada Cyclone Feed Pump 2.0.1 harus sudah dilakukan inspeksi preventif, karena telah beroperasi 664.8 jam, dan perbaikan yang harus lakukan maksimal 3.4997 jam setiap kali dilakukan shutdown.

  17. PENENTUAN POSISI SUMBER PROSPEK PANAS BUMI BERDASARKAN DATA ANOMALI MAGNETDI DAERAH AKESAHU, PULAU TIDORE, MALUKU UTARA

    Directory of Open Access Journals (Sweden)

    Rohima Wahyu Ningrum

    2015-04-01

    Full Text Available Penelitian geofisika dengan menggunakan metode magnetik yaitu dengan alat Magnetometer Fluxgate. Luas daerah penyelidikan ± 1 km x 1 km, dibatasi oleh koordinat geografis antara 127° 27’ 42” s/d 127° 27’ 43” BT dan 0° 43’ 17” s/d 0° 43’ 19” LU . Penelitian ini bertujuan untuk mengetahui posisi sumber prospek panas bumi di daerah Akesahu, Pulau Tidore berdasarkan data anomali medan magnet. Data medan magnet total yang terukur adalah medan utama, medan luar dan medan anomali. Untuk menghilangkan efek-efek anomali dari medan magnet luar dan anomali medan magnet utama dilakukan perhitungan koreksi variasi harian, koreksi drift (kesalahan alat, koreksi penyesuaian, koreksi IGRF, dan pemisahan anomali residual dan regional. Hasil penelitian menunjukkan bahwa, daerah Akesahu, Pulau Tidore memiliki potensi panas bumi yang masih cukup besar yang ditandai dengan adanya manifestasi panas bumi berupa mata air panas. Anomali medan magnet daerah Akesahu ditunjukkan dengan adanya anomali yang rendah (0.90 sampai dengan -2163.09 nT yang memanjang arah utara-selatan di bagian Timur Pulau Tidore. Disamping itu di sekitar mata air panas Akesahu secara geologis didukung dengan adanya empat buah sesar normal. Ini sebagai sistem pengontrol panas bumi yang lainnya di Pulau Tidore.

  18. EVALUASI SARANA DAN PRASARANA BENGKEL PRAKTIK SMK TEKNIK PEMESINAN DI KOTA SEMARANG BERDASARKAN KEBUTUHAN KURIKULUM

    Directory of Open Access Journals (Sweden)

    Sudiyono Sudiyono

    2016-03-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui: (1 kondisi sarana dan prasarana bengkel praktik SMK teknik pemesinan di Kota Semarang berdasarkan persepsi guru pengampu dan tool men, (2 ting-kat kesesuaiannya terhadap standar minimal Permendiknas Nomor 40 tahun 2008, dan (3 tingkat kecukupannya terhadap kebutuhan kurikulum. Penelitian ini merupakan penelitian evaluasi dengan model evaluasi diskrepansi. Pendekatan yang digunakan yaitu deskriptif kuantitatif dengan analisis persentase. Teknik pengumpulan data menggunakan analisis dokumen, wawancara, dan observasi. Teknik analisis yang digunakan adalah statistik deskriptif. Hasil penelitian menunjukkan bahwa: (1 Kondisi sarana dan prasarana bengkel praktik SMK Teknik Pemesinan di Kota Semarang menurut persepsi guru pengampu dan tool men pada SMK negeri maupun swasta sudah dinyatakan layak, (2 Prasarana pada SMK negeri sesuai dengan standar minimal, sedangkan pada SMK swasta cukup sesuai, (3 Sarana pada SMK negeri sangat sesuai dengan standar mini-mal, sedangkan pada SMK swasta sesuai standar, (4 Prasarana pada SMK negeri cukup meme-nuhi kebutuhan kurikulum, sedangkan pada SMK swasta kurang memenuhi, (5 Sarana pada SMK negeri cukup memenuhi kebutuhan kurikulum, sedangkan pada SMK swasta kurang memenuhi.

  19. Neutronics analysis of the DHCE experiment in ATR-ITV

    Energy Technology Data Exchange (ETDEWEB)

    Gomes, I.C.; Smith, D.L.; Tsai, H. [Argonne National Lab., IL (United States)

    1997-08-01

    The preliminary analysis of the DHCE experiments in the ITV and ATR was performed and its was concluded that such a vehicle is suitable for this kind of experiment. It is recommended to place an extra filter material in the thermocouple sleeve (such as B-10), to improve the helium to dpa ratio profile during irradiation. Also, it was concluded that a preliminary estimation of period of time for replacement of the external filter would be around 5 dps`s.

  20. Recent ATR and fusion algorithm improvements for multiband sonar imagery

    Science.gov (United States)

    Aridgides, Tom; Fernández, Manuel

    2009-05-01

    An improved automatic target recognition processing string has been developed. The overall processing string consists of pre-processing, subimage adaptive clutter filtering, normalization, detection, data regularization, feature extraction, optimal subset feature selection, feature orthogonalization and classification processing blocks. The objects that are classified by the 3 distinct ATR strings are fused using the classification confidence values and their expansions as features, and using "summing" or log-likelihood-ratio-test (LLRT) based fusion rules. The utility of the overall processing strings and their fusion was demonstrated with new high-resolution three-frequency band sonar imagery. The ATR processing strings were individually tuned to the corresponding three-frequency band data, making use of the new processing improvement, data regularization; this improvement entails computing the input data mean, clipping the data to a multiple of its mean and scaling it, prior to feature extraction and resulted in a 3:1 reduction in false alarms. Two significant fusion algorithm improvements were made. First, a nonlinear exponential Box-Cox expansion (consisting of raising data to a to-be-determined power) feature LLRT fusion algorithm was developed. Second, a repeated application of a subset Box-Cox feature selection / feature orthogonalization / LLRT fusion block was utilized. It was shown that cascaded Box-Cox feature LLRT fusion of the ATR processing strings outperforms baseline "summing" and single-stage Box-Cox feature LLRT algorithms, yielding significant improvements over the best single ATR processing string results, and providing the capability to correctly call the majority of targets while maintaining a very low false alarm rate.

  1. Preliminary Discrimination of Butter Adulteration by ATR-FTIR Spectroscopy

    Directory of Open Access Journals (Sweden)

    Lucian Cuibus

    2015-05-01

    Full Text Available The Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR  was applied for the discrimination of butter samples adulterated with solid fraction of palm oil. For FTIR fingerprinting of butter samples, with or without controlled additions of palm oil as adulterant was firstly obtained, using a Shimatsu Prestige 21 Spectrophotometer, including a horizontal diamond ATR accessory with reflection in the MIR region (3873-690 cm-1.The spiked butter samples including 0 level and seven increasing concentrations of palm fats, up to 50% were fingerprinted and the calibration curve was obtained (n=19. In parallel, the validation was realized using different set of spiked butter samples ranging 1-44.4 % of palm fat (n=7. Finally, an independent set of commercial samples was analized (n=14.Partial least squares (PLS model was used for statistical data processing in accordance with standard method. The value of the correlation coefficient (R2= 0.977 between actual and predicted values was statistically significant (p<0.001, considering the superposition of  "actual vs predicted” curves. This combined FTIR-PLS evaluation revealed that 3 out of  samples of butter were suspected of adulteration with palm oil, presented values 14 ranging 4-12%.In conclusion, ATR-FTIR methodology may offer an rapid evaluation of  butter samples authenticity. The low value for detection limit (3%palm oil in butter and the low limit of quantification (9.8% palm oil in butter confirms that ATR-FTIR spectroscopy  is a sensitive method to identify the adulteration of butter with  palm oil.   

  2. Neutronics analysis of the DHCE experiment in ATR-ITV

    International Nuclear Information System (INIS)

    The preliminary analysis of the DHCE experiments in the ITV and ATR was performed and its was concluded that such a vehicle is suitable for this kind of experiment. It is recommended to place an extra filter material in the thermocouple sleeve (such as B-10), to improve the helium to dpa ratio profile during irradiation. Also, it was concluded that a preliminary estimation of period of time for replacement of the external filter would be around 5 dps's

  3. Polynomial chaos theory for performance evaluation of ATR systems

    Science.gov (United States)

    DeVore, Michael D.; Bateman, Alec J.

    2010-04-01

    The development of a more unified theory of automatic target recognition (ATR) has received considerable attention over the last several years from individual researchers, working groups, and workshops. One of the major benefits expected to accrue from such a theory is an ability to analytically derive performance metrics that accurately predict real-world behavior. Numerous sources of uncertainty affect the actual performance of an ATR system, so direct calculation has been limited in practice to a few special cases because of the practical difficulties of manipulating arbitrary probability distributions over high dimensional spaces. This paper introduces an alternative approach for evaluating ATR performance based on a generalization of NorbertWiener's polynomial chaos theory. Through this theory, random quantities are expressed not in terms of joint distribution functions but as convergent orthogonal series over a shared random basis. This form can be used to represent any finite-variance distribution and can greatly simplify the propagation of uncertainties through complex systems and algorithms. The paper presents an overview of the relevant theory and, as an example application, a discussion of how it can be applied to model the distribution of position errors from target tracking algorithms.

  4. AGR-2 Data Qualification Report for ATR Cycle 154B

    Energy Technology Data Exchange (ETDEWEB)

    Binh Pham; Jeff Einerson

    2014-01-01

    This report provides the data qualification status of Advanced Gas Reactor-2 (AGR-2) fuel irradiation experimental data from Advanced Test Reactor (ATR) Cycle 154B as recorded in the Nuclear Data Management and Analysis System (NDMAS). This is the last cycle of AGR-2 irradiation, as the test train was pulled from the ATR core during the outage portion of ATR Cycle 155A. The AGR-2 data streams addressed in this report include thermocouple (TC) temperatures, sweep gas data (flow rates including new Fission Product Monitoring (FPM) downstream flows from Fission Product Monitoring System (FPMS) detectors, pressure, and moisture content), and FPMS data (release rates and release-to-birth rate ratios [R/Bs]) for each of the six capsules in the AGR-2 experiment. The final data qualification status for these data streams is determined by a Data Review Committee (DRC) comprised of AGR technical leads, Sitewide Quality Assurance (QA), and NDMAS analysts. The Data Review Committee reviewed the data acquisition process, considered whether the data met the requirements for data collection as specified in QA-approved Very High Temperature Reactor (VHTR) data collection plans, examined the results of NDMAS data testing and statistical analyses, and confirmed the qualification status of the data as given in this report.

  5. The ABC transporter MgAtr4 is a virulence factor of Mycosphaerella graminicola that affects colonization of substomatal cavities in wheat leaves

    NARCIS (Netherlands)

    Stergiopoulos, I.; Zwiers, L.H.; Waard, de M.A.

    2003-01-01

    The role in virulence of the ATP-binding cassette (ABC) transporters MgAtr1, MgAtr2, MgAtr3, MgAtr4, and MgAtr5 from Mycosphaerella graminicola was analyzed by gene disruption or replacement on seedlings of the susceptible wheat cultivar Obelisk. Disruption strains of MgAtr1 and MgAtr2 and replaceme

  6. Diversitas Genetik Anopheles balabacensis, Baisas di Berbagai Daerah Indonesia Berdasarkan Sekuen Gen ITS 2 DNA Ribosom

    Directory of Open Access Journals (Sweden)

    Widiarti Widiarti

    2016-05-01

    dilaporkan sebagai spesies kompleks di berbagai negara, akan tetapi belum banyak dilaporkan di Indonesia. Penanggulangan malaria agar lebih efektif perlu adanya perbaikan dan pendekatan strategi dalam pengendalian vektor, termasuk sangat diperlukan adanya pemahaman terhadap spesies dan bioekologinya. Tujuan penelitian adalah untuk : a. Mengidentifikasi secara molekuler nyamuk An. balabacensis yang dicurigai sebagai spesies kompleks berdasarkan sekuen ITS2 DNA ribosom, b. Mengetahui diversitas genetik nyamuk An. balabacensis dari daerah endemis dan non endemis dengan jarak geografis yang berbeda, c. Mengetahui kekerabatan genetik (jarak taksonomi nyamuk An. balabacensis dari berbagai daerah di Indonesia dengan merekonstruksi pohon filogenetik. Hasil penelitian menunjukkan bahwa An. balabacensis di Indonesia merupakan spesies kompleks simpatrik dan allopatrik. Ada perbedaan kekerabatan genetikyang cukup jauh diantara populasi An. balabacensis di Pusuk Lestari, wilayah Puskesmas Meninting, Lombok Barat, NTB yang merupakan simpatrik kompleks. Berdasarkan hubungankekerabatan An. leucosphyrus group, An. balabacensis dari Berjoko, Kabupaten Nunukan menunjukkan kecenderungan terpisah cukup jauh dibandingkan dengan An. balabacensiskompleks lainnya yang berasal dari Jawa Tengah dan Lombok, NTB.Kata kunci : An. balabacensis, variasi genetik, ITS2 DNA ribosom

  7. STUDI POTENSI ENERGI GEOTHERMAL BLAWAN- IJEN, JAWA TIMUR BERDASARKAN METODE GRAVITY

    Directory of Open Access Journals (Sweden)

    Raehanayati Raehanayati

    2013-11-01

    Full Text Available Penelitian ini merupakan studi awal untuk menentukan daerah yang memiliki potensi   panasbumi   berdasarkan   pengukuran   gayaberat   di   Blawan-Ijen,   Jawa   Timur. Pengukuran data primer dilakukan dengan menggunakan Gravitimeter LaCoste & Romberg tipe G-1053. Data anomali Bouger dari hasil perhitungan koreksi-koreksi metode gayaberat kemudian dibawa ke bidang datar selanjutnya dilakukan pemisahan anomali regional dan anomali sisa dengan menggunakan metode kontinuasi ke  atas. Hasil interpretasi terhadap anomali sisa yang dilakukan pada tiga penampang adalah penampang A-A’ nilai densitasnya yaitu: ρ1=2.58 gr/cm3, ρ2=2.80 gr/cm3 , ρ3=2.67 gr/cm3, dan ρ4=2.69 gr/cm3, sedangkan untuk penampang B-B’ nilai densitasnya adalah ρ1=2.58 gr/cm3, ρ2=2.82 gr/cm3, ρ3=2.67 gr/cm3, dan untuk penampang C-C’  nilai  densitasnya yaitu  ρ1=2.585 gr/cm3,  ρ2=2.82 gr/cm3,  ρ3=2.67 gr/cm3 dan ρ4=2.684 gr/cm3. Dari hasil pemodelan 2D dan 3D dapat terlihat bahwa pada daerah yang memiliki manifestasi air panas didominasi oleh batuan ρ1 karena memiliki nilai densitas paling rendah yang berada pada daerah Blawan. Sehingga dapat disimpulkan bahwa reservoir panasbumi  daerah  Blawan-Ijen  didominasi  oleh  batuan  yang  memiliki  porositas  tinggi (densitas rendah dan tingkat permeabilitasnya tinggi dengan jumlah volume sebesar 101.20 juta m3.

  8. Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection

    DEFF Research Database (Denmark)

    Carson, Christian T; Orazio, Nicole I; Lee, Darwin V;

    2009-01-01

    replication centres, but there is minimal ATR activation. We show that the Mre11/Rad50/Nbs1 (MRN) complex is recruited to viral centres only during infection with adenoviruses lacking the early region E4 and ATR signaling is activated. This suggests a novel requirement for the MRN complex in ATR activation......The protein kinases ataxia-telangiectasia mutated (ATM) and ATM-Rad3 related (ATR) are activated in response to DNA damage, genotoxic stress and virus infections. Here we show that during infection with wild-type adenovirus, ATR and its cofactors RPA32, ATRIP and TopBP1 accumulate at viral...... for immobilization of the MRN complex and show that this prevents ATR signaling during adenovirus infection. We propose that immobilization of the MRN damage sensor by E4orf3 protein prevents recognition of viral genomes and blocks detrimental aspects of checkpoint signaling during virus infection....

  9. Evaluating E-business in IT company : ATR Soft Oy

    OpenAIRE

    Franc, Eric

    2014-01-01

    The internet is now at an age where company can’t ignore having their products and services advertised on this channel. Internet is becoming a new way to gain and attract customers. The objective of this study is to evaluate if implementation of E-business could be viable for an IT software company. This study was commissioned by ATR Soft Oy and it begins with an overview of the company business model and product line. The theory section examines frameworks regarding E-business transforma...

  10. UCSB ATR-­NSUF Irradiation DMC Sample Inspection Report

    Energy Technology Data Exchange (ETDEWEB)

    Saleh, Tarik A. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Quintana, Matthew Estevan [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Romero, Tobias J. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-02-23

    A variety of tensile samples of Ferritic and Oxide Dispersion Strengthened (ODS or nanoferritic) steels were placed the ATR reactor over 2 Years achieving doses of roughly 4-6 dpa at temperatures of roughly 290°C. After irradiation, samples were shipped from the MFC hot cells at Idaho National Laboratory (INL) to the Wing 9 hot cells in the CMR facility at Los Alamos National Laboratory. Samples were cleaned to removed alpha contamination from the MFC hot cells, and then, as needed removed from their irradiation containers, sorted and inspected. This report will summarize the inspection of the Disc Multipurpose Coupon (DMC) inspection from packet 7-1.

  11. ATR-FTIR Studies on the Packing Film%一种包装膜的ATR-FTIR的剖析

    Institute of Scientific and Technical Information of China (English)

    孙瑞卿; 张汉辉; 杨融生

    2001-01-01

    FTIR and ATR-FTIR of the packing film have been studied.The dimension of thickness is about 0.034 m/m,the right and wrong sides of this packing film is composed of polypropylene and polyethylene glycol terephthalate, respectively.%在FTIR中,使用透射谱及水平ATR对进口的一种包装膜进行分析,该膜的厚度大约为0.034 m/m,膜的两面分别由等规聚丙烯和聚对苯二甲酸乙二醇酯组成。

  12. Proses Berpikir Siswa SD dalam Melakukan Estimasi Masalah Berhitung Berdasarkan Jenis Kelamin

    Directory of Open Access Journals (Sweden)

    Muh. Rizal

    2013-01-01

    Full Text Available Abstract: The Thinking Process of Primary-school Students of Different Sexes in Estimating Arithmetic Problems. This case study is intended to explore the thinking process of the fifth-year students of different sexes in estimating arithmetic problems. Two students, one male and one female, belonging to the high achievers in a mathematics test were selected for the study and then interviewed, assigned to solve arithmetic problems, and finally asked to think aloud their thinking process. The study reveals that the thinking process of both the male and female subjects is in the form of accommodation as they ap­proached the arithmetic problems by repeatedly reading the tasks. Though employing the same thinking process, in the planning stage, the male subject made use of rounding and compatible number strategies, whereas the female one used only rounding strategy. In implementing the plan, the male subject em­ployed mental counting through assimilation, but the female one used algorithm through accommoda­tion. In the evaluating stage, the male subject traced back his work through mental counting, while the female one utilized reverse operations. Abstrak: Proses Berpikir Siswa SD dalam Melakukan Estimasi Masalah Berhitung Berdasarkan Jenis Kelamin. Penelitian ini ingin mendeskripsikan proses berpikir siswa laki-laki dan perempuan yang berkemampuan matematika tinggi dalam melakukan estimasi. Penelitian dilakukan di kelas V SD, dengan subjek satu siswa laki-laki dan satu siswa perempuan yang memiliki skor 75 ke atas dalam tes kemampuan matematika. Data dikumpulkan dengan wawancara, pemberian tes dan think aloud. Analisis dilakukan dengan menelaah seluruh data, reduksi data, pengolompokan data, kategorisasi, pengkodean, dan pemeriksaan kredibilitas data dengan triangulasi waktu. Hasil penelitian menunjukkan bahwa proses berpikir keduanya adalah akomodasi. Dalam membuat rencana, laki-laki menggunakan rounding dan compatible number strategy, sedangkan

  13. Measured Thermal and Fast Neutron Fluence Rates for ATF-1 Holders During ATR Cycle 157D

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Larry Don [Idaho National Lab. (INL), Idaho Falls, ID (United States); Miller, David Torbet [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2016-03-01

    This report contains the thermal (2200 m/s) and fast (E>1MeV) neutron fluence rate data for the ATF-1 holders located in core for ATR Cycle 157D which were measured by the Radiation Measurements Laboratory (RML) as requested by the Power Reactor Programs (ATR Experiments) Radiation Measurements Work Order.

  14. ATR-FTIR Spectroscopy in the Undergraduate Chemistry Laboratory: Part I--Fundamentals and Examples

    Science.gov (United States)

    Schuttlefield, Jennifer D.; Grassian, Vicki H.

    2008-01-01

    Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy is a useful technique for measuring the infrared spectra of solids and liquids as well as probing adsorption on particle surfaces. Several examples of the use of FTIR-ATR spectroscopy in different undergraduate chemistry laboratory courses are presented here. These…

  15. Partial duplications of the ATRX gene cause the ATR-X syndrome.

    Science.gov (United States)

    Thienpont, Bernard; de Ravel, Thomy; Van Esch, Hilde; Van Schoubroeck, Dominique; Moerman, Philippe; Vermeesch, Joris Robert; Fryns, Jean-Pierre; Froyen, Guy; Lacoste, Caroline; Badens, Catherine; Devriendt, Koen

    2007-10-01

    ATR-X syndrome is a rare syndromic X-linked mental retardation disorder. We report that some of the patients suspected of ATR-X carry large intragenic duplications in the ATRX gene, leading to an absence of ATRX mRNA and of the protein. These findings underscore the need for including quantitative analyses to mutation analysis of the ATRX gene.

  16. ARF and ATM/ATR cooperate in p53-mediated apoptosis upon oncogenic stress

    International Nuclear Information System (INIS)

    Induction of apoptosis is pivotal for eliminating cells with damaged DNA or deregulated proliferation. We show that tumor suppressor ARF and ATM/ATR kinase pathways cooperate in the induction of apoptosis in response to elevated expression of c-myc, β-catenin or human papilloma virus E7 oncogenes. Overexpression of oncogenes leads to the formation of phosphorylated H2AX foci, induction of Rad51 protein levels and ATM/ATR-dependent phosphorylation of p53. Inhibition of ATM/ATR kinases abolishes both induction of Rad51 and phosphorylation of p53, and remarkably reduces the level of apoptosis induced by co-expression of oncogenes and ARF. However, the induction of apoptosis is downregulated in p53-/- cells and does not depend on activities of ATM/ATR kinases, indicating that efficient induction of apoptosis by oncogene activation depends on coordinated action of ARF and ATM/ATR pathways in the regulation of p53

  17. ANALISIS TERHADAP PERLUNYA PENYESUAIAN LAPORAN KEUANGAN HISTORIS (CONVENTIONAL ACCOUNTING MENJADI BERDASARKAN TINGKAT HARGA UMUM (GENERAL PRICE LEVEL ACCOUNTING

    Directory of Open Access Journals (Sweden)

    Pwee Leng

    2002-01-01

    Full Text Available Generally, in conventional accounting, financial statements are based on the historical cost principle that assumes that prices (monetery unit are stable. Conventional accounting recognizes neither changes in the general price level nor changes in the specific price level. Consequently, if there are any changes in purchasing power such as in inflation period, the historical financial statement are not economically relevant and also income is usually overstated, and the fixed assets are usually understated. Actually, there are several methods on accounting for the effect of changing prices, such as constant accounting, current value accounting, and general price level accounting. General price level accounting will do restatement the components of financial statement to be a rupiah on a similar level of purchasing power, but without changes in accounting principles which using on conventional accounting. In practice, the controversy concerning the relevance of general price level accounting has been continuing. Pros and cons general price level accounting will be presented on this paper. Also the result of two researches concerning the influence of applied general price level accounting on the financial statement will be compared as considerations whether the general price level adjustment is necessary needed. Abstract in Bahasa Indonesia : Secara umum, dalam akuntansi konvensional, laporan keuangan disajikan berdasarkan nilai historis yang mengasumsikan bahwa harga-harga (unit moneter adalah stabil. Akuntansi konvensional tidak mengakui adanya perubahan tingkat harga umum maupun perubahan tingkat harga khusus. Sebagai konsekuensinya, jika terjadi perubahan daya beli seperti pada periode inflasi, maka laporan keuangan historis secara ekonomis tidaklah relevan. Pada periode ini pendapatan umumnya dinilai lebih tinggi sedangkan aktiva tetap dinilai lebih rendah. Sebenarnya, terdapat beberapa metode akuntansi mengenai pengaruh perubahan harga

  18. Analisis Deskriptif Soal Geometri dalam Buku Matematika Bilingual untuk Sekolah Menengah Pertama Kelas VIII Berdasarkan Kriteria International Assessment TIMSS 2007

    Directory of Open Access Journals (Sweden)

    Etik Rahayu

    2012-06-01

    Full Text Available Tujuan penelitian ini adalah untuk mendeskripsikan  domain kognitif dan aspek kognitif (required behavior soal matematika dalam Buku Matematika Bilingual untuk Sekolah Menengah Pertama (SMP Kelas VIII berdasarkan kriteria International Assessment TIMSS 2007 dan proporsinya. Metode penelitian yang digunakan adalah deskriptif kualitatif, dengan subjek penelitian adalah soal geometri dalam Buku Matematika Bilingual SMP. yang berjudul “Mathematics for Junior High School Grade VIII 1st Semester” dan “Mathematics for Junior High School Grade VIII 2nd Semester” karangan karangan M. Cholik Adinawan dan Sugijono. Pengumpulan data menggunakan metode observasi dan wawancara tentang penggunaan buku matematika bilingual yang paling banyak digunakan di kota semarang. Pedoman analisis soal berdasarkan kriteria International Assessment TIMSS 2007, dengan validasi hasil oleh ahli. Hasil penelitian menunjukkan bahwa soal yang dianalisis memuat satu hingga tujuh aspek kognitif. Sebagian besar soal memuat 4 aspek kognitif yaitu 44.04 %, diikuti soal dengan 3 aspek kognitif  yaitu 36, 42%,  soal dengan 2 aspek kognitif yaitu 14, 90%, kemudian 1,99% untuk soal dengan 1 atau 5 aspek kognitif, dan 0,33% untuk soal dengan 6 atau 7 aspek kognitif. Proporsi tinggi pada recall (28.26% dan compute (26.57%, diikuti dengan SRP (10.85%, implement (10.65%, retrieve (8.36%, recognize (6.17%, analyze (1.99%, measure (1.59%, generalize (1.09%, SNRP (1.00%, classify (0.80%, represent (0.80%, justify (0.80%, select (0.60%, model (0.30%, synthesis (0.20%. Secara keseluruhan berdasarkan International Assessment TIMSS 2007 soal yang termasuk domain knowing memiliki persentase paling tinggi (52.28%, domain knowing-applying (24.83%, domain knowing- reasoning (12.91%, dan hanya sedikit yang termasuk domain knowing-apllying-reasoning (3.97%. Serta terdapat 4 soal (1.32% yang mempunyai ketidaksesuaian penggunaan mathematics terms serta 1 soal (0

  19. Modern approaches in deep learning for SAR ATR

    Science.gov (United States)

    Wilmanski, Michael; Kreucher, Chris; Lauer, Jim

    2016-05-01

    Recent breakthroughs in computational capabilities and optimization algorithms have enabled a new class of signal processing approaches based on deep neural networks (DNNs). These algorithms have been extremely successful in the classification of natural images, audio, and text data. In particular, a special type of DNNs, called convolutional neural networks (CNNs) have recently shown superior performance for object recognition in image processing applications. This paper discusses modern training approaches adopted from the image processing literature and shows how those approaches enable significantly improved performance for synthetic aperture radar (SAR) automatic target recognition (ATR). In particular, we show how a set of novel enhancements to the learning algorithm, based on new stochastic gradient descent approaches, generate significant classification improvement over previously published results on a standard dataset called MSTAR.

  20. Deep convolutional neural networks for ATR from SAR imagery

    Science.gov (United States)

    Morgan, David A. E.

    2015-05-01

    Deep architectures for classification and representation learning have recently attracted significant attention within academia and industry, with many impressive results across a diverse collection of problem sets. In this work we consider the specific application of Automatic Target Recognition (ATR) using Synthetic Aperture Radar (SAR) data from the MSTAR public release data set. The classification performance achieved using a Deep Convolutional Neural Network (CNN) on this data set was found to be competitive with existing methods considered to be state-of-the-art. Unlike most existing algorithms, this approach can learn discriminative feature sets directly from training data instead of requiring pre-specification or pre-selection by a human designer. We show how this property can be exploited to efficiently adapt an existing classifier to recognise a previously unseen target and discuss potential practical applications.

  1. Thermal Analysis of Irradiation Experiments in the ATR

    Energy Technology Data Exchange (ETDEWEB)

    Paul Murray

    2012-09-01

    Reactor material testing in the INL's Advanced Test Reactor (ATR) involves modeling and simulation of each experiment to accurately determine the irradiation temperature. This paper describes thermal analysis of capsule experiments using gas gap temperature control and provides data on recent material tests that validate the modeling results. Static capsule experiments and lead-out capsule experiments are discussed. The source of temperature variation in capsule experiments and ways to mitigate these variations are also explained. Two examples of instrumented lead-out capsule experiments, TMIST-1 and UCSB-2, are presented. A comparison of measured and calculated temperatures is used to validate the thermal models and to ascertain the accuracy of the calculated temperature.

  2. Wurfelspiel-based training data methods for ATR

    Science.gov (United States)

    Peterson, James K.

    2004-09-01

    A data object is constructed from a P by M Wurfelspiel matrix W by choosing an entry from each column to construct a sequence A0A1"AM-1. Each of the PM possibilities are designed to correspond to the same category according to some chosen measure. This matrix could encode many types of data. (1) Musical fragments, all of which evoke sadness; each column entry is a 4 beat sequence with a chosen A0A1A2 thus 16 beats long (W is P by 3). (2) Paintings, all of which evoke happiness; each column entry is a layer and a given A0A1A2 is a painting constructed using these layers (W is P by 3). (3) abstract feature vectors corresponding to action potentials evoked from a biological cell's exposure to a toxin. The action potential is divided into four relevant regions and each column entry represents the feature vector of a region. A given A0A1A2 is then an abstraction of the excitable cell's output (W is P by 4). (4) abstract feature vectors corresponding to an object such as a face or vehicle. The object is divided into four categories each assigned an abstract feature vector with the resulting concatenation an abstract representation of the object (W is P by 4). All of the examples above correspond to one particular measure (sad music, happy paintings, an introduced toxin, an object to recognize)and hence, when a Wurfelspiel matrix is constructed, relevant training information for recognition is encoded that can be used in many algorithms. The focus of this paper is on the application of these ideas to automatic target recognition (ATR). In addition, we discuss a larger biologically based model of temporal cortex polymodal sensor fusion which can use the feature vectors extracted from the ATR Wurfelspiel data.

  3. Gambaran Slide Malaria Berdasarkan Sediaan Darah dari Kepulauan Siberut Mentawai Periode Oktober 2011 – Januari 2012

    Directory of Open Access Journals (Sweden)

    Adeline Sacharissa Firdaus

    2014-05-01

    Full Text Available AbstrakMalaria adalah penyakit penting yang saat ini telah menjadi masalah kesehatan dunia dan endemik di 105 negara salah satunya Indonesia. Indonesia memiliki banyak kepulauan yang tersebar salah satunya yaitu Kepulauan Mentawai. Kepulauan Mentawai merupakan daerah endemi malaria yang terdiri atas 4 pulau salah satunya yaitu Kepulauan Siberut Mentawai. Tujuan dari penelitian ini adalah untuk mengetahui Insiden kejadian malaria di Kepulauan Siberut Mentawai periode Oktober 2012 – Januari 2012. Desain penelitian yaitu deskriptif dan observational. Sediaan darah yang berasal dari Puskesmas Muara Siberut Kepulauan Siberut Mentawai dikirim ke Laboratorium Parasitologi Fakultas Kedokteran Universitas Andalas untuk diperiksa. Penelitian dilakukan dengan cara pemeriksaan secara mikroskopik sediaan darah tebal dan tipis dari sampel darah tepi yang telah dipulas dengan pewarnaan Giemsa untuk mengetahui berapa insiden kejadian malaria, distribusi malaria menurut jenis kelamin, distribusi malaria menurut jenis plasmodium dan Parasite Count. Seluruh Sediaan darah berjumlah 106 sediaan darah dan 32 diantaranya positif malaria. Berdasarkan jenis kelamin ditemukan pada perempuan 17 sediaan darah dan laki-laki sebanyak 15 sediaan darah. jenis Plasmodium yang ditemukan adalah Plasmodium falciparum sebanyak 20 sediaan darah dan Plasmodium vivax sebanyak 12 sediaan darah. Menurut parasite count 14 sediaan darah diantaranya merupakan infeksi ringan dan 6 sediaan darah lainnya merupakan infeksi berat. Kesimpulan penelitian ini adalah (1 kejadian positif malaria ditemukan sebanyak 30,2%, (2 Insiden kejadian malaria lebih tinggi pada perempuan dibandingkan laki-laki, (3 Hanya ditemukan jenis Plasmodium falciparum dan Plasmodium vivax pada penelitian, (4 Derajat infeksi ringan kejadiannya lebih tinggi dibandingkan infeksi berat.Kata kunci: Malaria, Plasmodium falciparum, Plasmodium vivax, Parasit countAbstractMalaria is an important disease that has become a global

  4. Kajian Biblika Kecemburuan Allah Terhadap Penyembahan Berhala Berdasarkan Keluaran 20:4-6

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    Queency Christie Wauran

    2015-09-01

    Full Text Available Dalam Perjanjian Lama kecemburuan Allah selalu berada dalam konteks penyembahan berhala. Hukum Taurat yang kedua dengan jelas melarang penyembahan kepada patung oleh karena alasan ini yaitu Allah yang cemburu. Tujuan penulisan artikel ini adalah untuk mengetahui arti kecemburuan Allah terhadap penyembahan berhala berdasarkan kitab Keluaran 20:4-6 dan implikasinya bagi kehidupan orang percaya. Adapun kesimpulan artikel ini adalah pertama, kecemburuan Allah terhadap penyembahan berhala menyatakan bahwa Allah tidak dapat direpresentasikan dalam bentuk apapun karena kecemburuan Allah ini menyatakan bahwa Ia adalah Allah yang kudus. Dengan demikian, hanya Allah satu-satunya yang harus disembah oleh orang percaya. Kedua, kecemburuan Allah terhadap penyembahan berhala menyatakan bahwa tidak boleh ada objek penyembahan yang lain selain kepada Allah karena penyembahan kepada berhala menandakan perzinaan rohani yang mengakibatkan kecemburuan Allah. Oleh karena itu, orang percaya harus menolak segala bentuk penyembahan berhala. Ketiga, penyembahan kepada berhala mendatangkan kecemburuan Allah karena kecemburuan Allah ini menyatakan kesetiaan-Nya atas perjanjian dengan umat-Nya. Dengan demikian, kecemburuan Allah seharusnya menjadi dasar dari penyembahan kepada Allah. Keempat, kecemburuan Allah atas penyembahan berhala mendatangkan hukuman karena kecemburuan Allah menyatakan keadilan-Nya dalam hal pemberian hukuman sebagai konsekuensi bagi penyembah berhala. Kelima, kecemburuan Allah atas penyembahan berhala menyatakan bahwa Allah mengasihi umat-Nya dan sekaligus menyatakan keadilan Allah dalam hal pemberian berkat bagi yang mengasihi-Nya. Kasih Allah mendasari hubungan-Nya dengan umat-Nya, dengan demikian kasih juga menjadi dasar hubungan orang percaya dengan Allah.In the Old Testament, the jealousy of God is always in the context of worshipping idols. The second commandment clearly prohibits the worshipping of statues for this reason, that God is jealous

  5. Analisis Isi SMS Iklan Layanan Telekomunikasi Operator Telkomsel, Indosat dan Xl berdasarkan Etika Pariwara Indonesia Periode 2013

    Directory of Open Access Journals (Sweden)

    Danang Trijayanto

    2014-09-01

    Full Text Available Perkembangan teknologi komunikasi merevolusi aktivitas pemasaran ke mobile marketing, salah satunya adalah dengan SMS advertising. Badan usaha yang cenderung menggunakan cara pemasaran tersebut adalah operator selular dengan iklan produk layanan telekomunikasinya. Permasalahannya adalah strategi pemasaran produk telekomunikasi yang cenderung banyak merugikan pelanggan karena ketidaksesuaian antara isi iklan dan produk yang diperoleh, yang dipengaruhi oleh kelengkapan dan kejelasan informasi produk. Salah satu aturan yang menjadi dasar dalam aktivitas SMS advertising adalah berdasarkan Etika Pariwara Indonesia.  Penelitian  ini   meneliti  bagaimanakah  Penerapan aturan tersebut terhadap isi SMS iklan produk telekomunikasi yang dikirimkan oleh provider selular GSM prabayar pada bulan Juli hingga Nopember 2013.  Hasil  Penelitian menunjukkan bahwa dalam mengiklankan produk telekomunikasi melalui SMS advertising, para operator selular belum menerapkan regulasi dan etika pemasaran dengan baik. Kekurangan informasi yang belum lengkap terdapat pada informasi syarat kontrak yang menjadi informasi dasar hak dan kewajiban yang harus dipenuhi oleh pelanggan.

  6. Mutation of serine 1333 in the ATR HEAT repeats creates a hyperactive kinase.

    Directory of Open Access Journals (Sweden)

    Jessica W Luzwick

    Full Text Available Subcellular localization, protein interactions, and post-translational modifications regulate the DNA damage response kinases ATR, ATM, and DNA-PK. During an analysis of putative ATR phosphorylation sites, we found that a single mutation at S1333 creates a hyperactive kinase. In vitro and in cells, mutation of S1333 to alanine (S1333A-ATR causes elevated levels of kinase activity with and without the addition of the protein activator TOPBP1. S1333 mutations to glycine, arginine, or lysine also create a hyperactive kinase, while mutation to aspartic acid decreases ATR activity. S1333A-ATR maintains the G2 checkpoint and promotes completion of DNA replication after transient exposure to replication stress but the less active kinase, S1333D-ATR, has modest defects in both of these functions. While we find no evidence that S1333 is phosphorylated in cultured cells, our data indicate that small changes in the HEAT repeats can have large effects on kinase activity. These mutants may serve as useful tools for future studies of the ATR pathway.

  7. Experimental active control of sound in the ATR 42

    Science.gov (United States)

    Paonessa, A.; Sollo, A.; Paxton, M.; Purver, M.; Ross, C. F.

    Passenger comfort is becoming day by day an important issue for the market of the regional turboprop aircraft and also for the future high speed propeller driven aircraft. In these aircraft the main contribution to the passenger annoyance is due to the propeller noise blade passing frequency (BPF) and its harmonics. In the recent past a detailed theoretical and experimental work has been done by Alenia Aeronautica in order to reduce the noise level in the ATR aircraft passenger cabin by means of conventional passive treatments: synchrophasing of propellers, dynamic vibration absorbers, structural reinforcements, damping materials. The application of these treatments has been introduced on production aircraft with a remarkable improvement of noise comfort but with a significant weight increase. For these reasons, a major technology step is required for reaching passenger comfort comparable to that of jet aircraft with the minimum weight increase. The most suitable approach to this problem has been envisaged in the active noise control which consists in generating an anti-sound field in the passenger cabin to reduce the noise at propeller BPF and its harmonics. The attenuation is reached by means of a control system which acquires information about the cabin noise distribution and the propeller speed during flight and simultaneously generates the signals to drive the speakers.

  8. 皮革产品的ATR-FTIR快速鉴定%High-Speed Examination of Leather Products by ATR-FTIR

    Institute of Scientific and Technical Information of China (English)

    张红雨; 徐琳; 王乃岩

    2004-01-01

    分析和比较真皮和人造革的ATR-FTIR红外谱图表明,两者的特征峰峰位并不重叠或干扰,其结构可以区分.用ATR-FTIR鉴定皮革产品具有准确、灵敏、快速、简便、对产品无损伤、无需预处理、易实现现场自动化等优点.

  9. Neutron dosimetry and damage calculations for the ATR-A1 irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Greenwood, L.R.; Ratner, R.T. [Pacific Northwest National Lab., Richland, WA (United States)

    1998-09-01

    Neutron fluence measurements and radiation damage calculations are reported for the collaborative US/Japan ATR-A1 irradiation in the Advanced Test Reactor (ATR) at Idaho National Engineering Laboratory (INEL). The maximum total neutron fluence at midplane was 9.4 {times} 10{sup 21} n/cm{sup 2} (5.5 {times} 10{sup 21} n/cm{sup 2} above 0.1 MeV), resulting in about 4.6 dpa in vanadium.

  10. Evaluasi Komponen Jalan Rel Berdasarkan Passing Tonnage dan Analisis Kebutuhan Pemeliharaan Tahunan Jalan Rel dengan Analisa JO Tahun 2011 (Studi Kasus Jalan Rel Lintas Medan – Tebing Tinggi)

    OpenAIRE

    Panjaitan, Herbet

    2014-01-01

    Jalan rel yang menghubungkan Medan – Tebing Tinggi merupakan lintasan jalan rel sepanjang 80,542 kilometer yang dilewati 40 kereta api setiap harinya, sehingga akan menurunkan kualitas pelayanan komponen jalan rel kereta api. Evaluasi komponen rel terpasang pada jalan rel kereta api dilakukan dengan menghitung daya angkut lintas jalan rel untuk satu tahun, kemudian membandingkan komponen jalan rel terpasang berdasarkan standar perencanaan jalan rel sesuai dengan klasifikasi kelas jalan r...

  11. Hubungan Keteraturan Penggunaan Kortikosteroid Inhalasi dengan Tingkat Kontrol Asma Pasien Berdasarkan ACT di Poliklinik Paru RSUP Dr. M. Djamil Padang

    Directory of Open Access Journals (Sweden)

    Wulan Prisilla Prisilla

    2016-01-01

    Full Text Available AbstrakAsma merupakan penyakit yang didasari oleh reaksi inflamasi pada saluran napas yang dapat dicegah dengan kortikosteroid inhalasi. Asma sukar disembuhkan, sehingga tujuan penatalaksanaan asma adalah asma terkontrol. Penilaian tingkat kontrol asma dapat dilakukan dengan menggunakan kuesioner Asthma Control Test  (ACT yang bersifat subjektif tetapi validitasnya telah diuji. Tujuan penelitian ini adalah menentukan hubungan keteraturan penggunaan kortikosteroid inhalasi dengan tingkat kontrol asma pasien berdasarkan ACT di Poliklinik Paru RSUP Dr. M. Djamil Padang. Penelitian cross sectional analytic ini telah dilakukan dari September hingga Desember 2013. Populasi adalah pasien berusia ≥14 tahun yang didiagnosis asma oleh dokter. Jumlah subjek penelitian sebanyak 96 orang. Data yang diperoleh diolah dan dianalisis menggunakan uji Kolmogorov-Smirnov. Insiden terbanyak asma berada pada kelompok usia lanjut (60,4%. Sebagian besar pasien tidak teratur menggunakan kortikosteroid inhalasi (63,5% dan pasien dengan asma tidak terkontrol memiliki proporsi tertinggi (59,4% . Analisis statistik menunjukkan bahwa terdapat hubungan yang bermakna antara keteraturan penggunaan kortikosteroid inhalasi dengan tingkat kontrol asma pasien berdasarkan ACT di Poliklinik Paru RSUP Dr. M. Djamil (p=0,002.Kata kunci: kortikosteroid inhalasi, ACT, tingkat control asma AbstractAsthma is a disease caused by an inflammatory reaction in the patient airways that can be prevented with inhaled corticosteroids. It is known that there is no cure for asthma, so the goal of asthma management is to obtain controlled asthma. The level of asthma control can be assessed by  using Asthma Control Test  (ACT questionnaire, this method is subjective but its validity has been tested. The objective of this study  was to determine the association between regularity of inhaled corticosteroids application and patient’s level of asthma control based on ACT in the Lung Clinic of Dr

  12. Identifikasi Carrier Bakteri Streptococcus β hemolyticus Group A pada Murid SD Negeri 13 Padang Berdasarkan Perbedaan Umur dan Jenis Kelamin

    Directory of Open Access Journals (Sweden)

    Fadhila Aini

    2016-01-01

    Full Text Available AbstrakStreptococcus β hemolyticus Grup A atau yang disebut juga Streptococcus pyogenes merupakan salah satu bakteri patogen yang banyak menginfeksi manusia.Bakteri ini dapat ditemukan sebagai carrier di saluran pernafasan terutama pada anak-anak, tidak menimbulkan penyakit tetapi berisiko untuk menyebarkan penyakit. Tujuan penelitian ini adalah menentukan jumlah carrier  bakteri Streptococcus β hemolyticus Grup A pada murid berdasarkan perbedaan umur dan jenis kelamin. Jenis penelitian ini adalah deskriptif cross-sectional dengan menggunakan sampel seluruh murid SD Negeri 13 Padang. Hasil penelitian adalah didapatkan 2 orang murid yang menderita carrier, yaitu pada kelompok usia>8-9 tahun dan >11 tahun. Berdasarkan jenis kelamin yang terdiri dari 54 orang laki-laki dan 50 orang perempuan, didapatkan 2 orang carrier yaitu hanya pada anak laki-laki. Hasil penelitian menunjukkan bahwa carrier bakteri Streptococcus β hemolyticus Group  A terdapat pada anak usia tersebut karena masih kurangnya pengetahuan tentang kebersihan. Carrier yang ditemukan hanya pada anak laki-laki kemungkinan disebabkan mereka lebih sering bermain di luar rumah dan terpapar dengan berbagai bakteri patogen dan kurang memperhatikan kebersihan diri.Kata kunci: carrier, streptococcus β hemolyticus grup A, umur, jenis kelamin AbstractGroup A Streptococcus β hemolyticus or also called Streptococcus pyogenes is one of many pathogenic bacteria that infect humans. These bacteria can be found as a carrier in the respiratory tract especially in children, do not cause disease but can be a risk for spreading the disease. This objective of this study was to determine the amount of the carrier of bacteria group A Streptococcus β hemolyticus based on age and gender differences. This research is a descriptive cross - sectional study using a sample of all students of SD Negeri 13 Padang. Based on the age of 104 students found that students who suffer 2 carrier, which is in the age

  13. TopBP1和ATR-ATRIP在细胞周期中的作用及联系%Role and Relationship of TopBP1 and ATR-ATRIP in the Cell Life Cycle

    Institute of Scientific and Technical Information of China (English)

    黄神安; 熊高飞; 张吉翔

    2006-01-01

    TopBP1(topoisomerase Ⅱ beta-binding protein 1)和ATR(ataxia-telangiectasia mutated-and rad3-related)-ATRIP(ATR-interacting protein)通过调节一些因子在DNA损伤检控点(DNA damage checkpoint)中起着关键的作用,其中TopBP1可以激活ATR-ATRIP的复合体.文章着重阐述了TopBP1和ATR-ATRIP如何调控细胞周期并发挥其维护基因组完整性的作用.

  14. Keragaman suweg (Amorphophallus campanulatus di wilayah eks karesidenan Surakarta berdasarkan karakter morfologi, anatomi dan pola pita isozim

    Directory of Open Access Journals (Sweden)

    MONARITA PERMATASARI

    2014-05-01

    Full Text Available Permatasari, Pitoyo A, Suratman. 2014. Keragaman suweg (Amorphophallus campanulatus di wilayah eks karesidenan Surakarta berdasarkan karakter morfologi, anatomi dan pola pita isozim. Bioteknologi 11: 11-18. The aims of this research were to find out the diversity and relationship among elephant yam in Surakarta region based on variation of morphological, anatomical and isozyme banding patterns characters. Total 20 samples were taken using random purposive sampling technique in different altitude of 7 districts. Morphological, anatomical and isozyme banding pattern data were analyzed descriptively. The steady data of quantitative characteristic from morphological, anatomical and isozyme banding pattern of binary data was analyzed using an Index of Similarity (IS and computed by the program Numerical Taxonomy and Multivariate Analysis System (NTSYS version 2.10. The result showed that the elephant yam in Surakarta region were diverse based on morphological, anatomical and isozyme banding pattern, especially in ratio and color of petiolus; tuber ratio; index of stomatal; thick epidermis; thick mesophyll; term and ratio of palisade; term rafida; 4 peroxidase and 5 esterase isozyme banding pattern. Analysis of relationship of elephant yam based on morphological, anatomical and isozyme band pattern characters showed the similarity coefficient of 64,85% contained two major group. The first group consist of Boyolali 1, Boyolali 3, Boyolali 4, Wonogiri 4, Wonogiri 2, Karanganyar 1, Karanganyar 2, Karanganyar 3, Sragen 2, while the other samples were separated from this group.

  15. GAMBARAN RISIKO TROMBOSIS VENA PROFUNDA (TVP PADA SALES PROMOTION GIRL (SPG BERDASARKAN KRITERIA WELLS DI KOTA DENPASAR

    Directory of Open Access Journals (Sweden)

    Shelly Silvia Bintang

    2015-01-01

    Full Text Available Pekerjaan sebagai sales promotion girl (SPG menuntut berdiri dalam posisi statis dalam waktu kerja yang cukup lama. Dalam sebuah jurnal kesehatan masyarakat tahun 2013 mendapatkan hasil semakin lama SPG bekerja maka semakin lama juga durasi mereka memakai sepatu hak tinggi semakin besar risiko untuk mengalami gangguan kesehatan yang disebabkan sepatu hak tinggi. Gangguan dari aliran darah atau trombosis vena profunda (TVP merupakan dampak yang ditimbulkan. Tujuan dari penelitian ini untuk mengetahui angka tinggi pemakaian sepatu hak tinggi, angka pemakaian stocking dan gambaran SPG di Kota Denpasar yang memiliki risiko rendah, menengah dan tinggi mengalami TVP. Penelitian ini menggunakan metode deskriptif potong lintang. Subyek penelitian ini adalah 62 pegawai SPG yang bekerja di swalayan di Kota Denpasar. Skor kriteria Wells didapatkan untuk mengetahui gambaran risiko probabilitas TVP. Hasil gambaran risiko TVP pada SPG di Kota Denpasar berdasarkan kriteria Wells didapatkan 30 orang pekerja (48,38% menunjukan risiko ringan, dan 30 orang pekerja lainnya (48,38% menunjukan risiko menengah menderita TVP, sedangkan 2 orang pekerja (3,24% menunjukan risiko tinggi. Sebagian besar pegawai SPG di kota Denpasar memiliki risiko rendah dan sedang probabilitas TVP.

  16. Perancangan Metamodel Registry Untuk Rekam Kesehatan Elektronik Berdasarkan Standar ISO/IEC 11179 (Studi Kasus : Rumah Sakit Haji Surabaya

    Directory of Open Access Journals (Sweden)

    Mochammad Baihaqi

    2014-09-01

    Full Text Available Pada saat ini pengelolaan data sudah menerapkan sistem rekam kesehatan elektronik dalam pelayanan kesehatan pada sebuah instansi atau lembaga kesehatan seperti Puskesmas, Rumah Sakit dan Klinik. Tetapi sistem tersebut belum diintegrasikan dengan baik dan dikembangkan untuk dapat men sharing data antar organisasi. Untuk itu pelayanan kesehatan secara elektronik melibatkan pengelolaan data yang perlu dirancang secara terstruktur dan terintegrasi agar sistem tersebut dapat berjalan dengan baik dalam menyelaraskan sistem data pada pelayanan kesehatan. Penelitian ini akan berfokus menggunakan terminology pada percancangan metamodel registry berdasarkan data dalam concepual data model (CDM yang menyesuaikan standar ISO/IEC 11179 bagian 4 dan 5. Perancangan metamodel registry dilakukan dengan beberapa tahap dengan menggunakan studi kasus Rumah Sakit Haji Surabaya. Metamodel registry dirancang menggunakan terminology konseptual data elemen tentang penamaan dan definisi data. Kemudian dilakukan verifikasi untuk memastikan perancangan metamodel registry sudah memenuhi standar ISO/IEC 11179 dan disusun dalam bentuk Data Dictionary untuk memperbaiki penamaan dan definisi dari conceptual data model (CDM Tugas Akhir ini diharapkan untuk memberikan gambaran pengelolaan data pada sistem data pelayanan kesehatan dan dapat diusulkan ke pusat kesehatan kota untuk memperbaiki sektor data kesehatan agar sistem data selaras dan dapat di sharing antar organisasi.

  17. Involvement of ATM/ATR-p38 MAPK cascade in MNNG induced G1-S arrest

    Institute of Scientific and Technical Information of China (English)

    Ke-Qing Zhu; Suo-Jiang Zhang

    2003-01-01

    AIM: To understand the effect of low concentration of Nmethyl-N′-nitro-nitrosoguanidine (MNNG), which is a widely distributed environmental mutagen and carcinogen especially for human gastric cancer, on DNA damage and to study its possible pathway in regulating cell cycle arrest.METHODS: The DNA damage effect was measured by Comet assay. A specific phospho-(Ser/Thr) ATM/ATR substrate antibody was used to detect the damage sensor by Western blot. p38 kinase activity was measured by direct kinase assay,and immunoprecipitation for the possible connection between ATM/ATR and p38 MAPK. Flow cytometry analysis and p38MAPK specific inhibitor SB203580 were combined to detect the possible cell cycle arrest by p38 MAPK.RESULTS: With the same low concentration MNNG exposure (0.2μM 2.5 h), Comet assays indicated that strand breaks accumulated, Western blot and kinase assay showed ATM/ATR and p38 kinase activated, immunoprecipitation showed phospho-ATM/ATR substrate antibody combined with both p38 MAPK antibody and phospho-p38 MAPK antibody, p38MAPK pathway was involved in the G1-S arrest.CONCLUSION: Activation of ATM/ATR by MNNG induced DNA damage leads to activation of p38 MAPK, which involves in the G1 checkpoint in mammalian cells.

  18. In-situ ATR-FTIR for characterization of thin biorelated polymer films

    Energy Technology Data Exchange (ETDEWEB)

    Müller, M., E-mail: mamuller@ipfdd.de [Leibniz Institute of Polymer Research Dresden (IPF Dresden), Hohe Straße 6, 01069 Dresden (Germany); Technical University of Dresden (TUD), Department of Chemistry and Food Chemistry, 01062 Dresden (Germany); Torger, B. [Leibniz Institute of Polymer Research Dresden (IPF Dresden), Hohe Straße 6, 01069 Dresden (Germany); Technical University of Dresden (TUD), Department of Chemistry and Food Chemistry, 01062 Dresden (Germany); Bittrich, E. [Leibniz Institute of Polymer Research Dresden (IPF Dresden), Hohe Straße 6, 01069 Dresden (Germany); Kaul, E.; Ionov, L. [Leibniz Institute of Polymer Research Dresden (IPF Dresden), Hohe Straße 6, 01069 Dresden (Germany); Technical University of Dresden (TUD), Department of Chemistry and Food Chemistry, 01062 Dresden (Germany); Uhlmann, P. [Leibniz Institute of Polymer Research Dresden (IPF Dresden), Hohe Straße 6, 01069 Dresden (Germany); Stamm, M. [Leibniz Institute of Polymer Research Dresden (IPF Dresden), Hohe Straße 6, 01069 Dresden (Germany); Technical University of Dresden (TUD), Department of Chemistry and Food Chemistry, 01062 Dresden (Germany)

    2014-04-01

    We present and review in-situ-attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic data from thin biorelated polymer films useful for the modification and functionalization of polymer and inorganic materials and discuss their applications related to life sciences. A special ATR mirror attachment operated by the single-beam-sample-reference (SBSR) concept and housing a homebuilt thermostatable flow cell was used, which allows for appropriate background compensation and signal to noise ratio. ATR-FTIR data on the reactive deposition of dopamine on inorganic model surfaces are shown. Information on the structure and deposition pathway for such bioinspired melanin-like films is provided. ATR-FTIR data on thermosensitive polymer brushes of poly(N-isopropylacrylamide) (PNIPAAM) is then presented. The thermotropic hydration and hydrogen bonding behavior of PNIPAAM brush films is described. Finally, ATR-FTIR data on biorelated polyelectrolyte multilayers (PEM) are given together with details on PEM growth and detection. Applications of these latter films for biopassivation/activation and local drug delivery are addressed.

  19. Evaluation of shooting distance by AFM and FTIR/ATR analysis of GSR.

    Science.gov (United States)

    Mou, Yongyan; Lakadwar, Jyoti; Rabalais, J Wayne

    2008-11-01

    The techniques of atomic force microscopy (AFM) and Fourier transform infrared attenuated total reflectance (FTIR/ATR) spectroscopy are applied to the analysis of gun-shot residue (GSR) to test their ability to determine shooting distance and discrimination of the powder manufacturers. AFM is a nondestructive technique that is capable of characterizing the shapes and size distributions of GSR particles with resolution down to less than a nanometer. This may be useful for estimation of the shooting distance. Our AFM images of GSR show that the size distribution of the particles is inversely proportional to the shooting distance. Discrimination of powder manufacturers is tested by FTIR/ATR investigation of GSR. Identifying the specific compounds in the GSR by FTIR/ATR was not possible because it is a mixture of the debris of several compounds that compose the residue. However, it is shown that the GSR from different cartridges has characteristic FTIR/ATR bands that may be useful in differentiating the powder manufacturers. It appears promising that the development of AFM and FTIR/ATR databases for various powder manufacturers may be useful in analysis and identification of GSR. PMID:18761553

  20. Status of ATR-A1 irradiation experiment on vanadium alloys and low-activation steels

    Energy Technology Data Exchange (ETDEWEB)

    Tsai, H.; Strain, R.V.; Gomes, I.; Smith, D.L. [Argonne National Lab., IL (United States); Matsui, H. [Tohoku Univ. (Japan)

    1996-10-01

    The ATR-A1 irradiation experiment was a collaborative U.S./Japan effort to study at low temperature the effects of neutron damage on vanadium alloys. The experiment also contained a limited quantity of low-activation ferritic steel specimens from Japan as part of the collaboration agreement. The irradiation started in the Advanced Test Reactor (ATR) on November 30, 1995, and ended as planned on May 5, 1996. Total exposure was 132.9 effective full power days (EFPDs) and estimated neutron damage in the vanadium was 4.7 dpa. The vehicle has been discharged from the ATR core and is scheduled to be disassembled in the next reporting period.

  1. Esophago-gastric motility and nutritional management in a child with ATR-X syndrome.

    Science.gov (United States)

    Watanabe, Toshihiko; Arai, Katsuhiro; Takahashi, Masataka; Ohno, Michinobu; Sato, Kaori; Fuchimoto, Yasushi; Wada, Takahiko; Ida, Shinobu; Kawahara, Hisayoshi; Kanamori, Yutaka

    2014-08-01

    X-linked alpha thalassemia mental retardation (ATR-X) syndrome is an X-linked recessive disorder that often involves gastrointestinal symptoms. Aspiration pneumonia related to gastroesophageal reflux has been reported as the major cause of death, but gastrointestinal function has not been well investigated. The present report describes a child with ATR-X syndrome who suffered from periodical episodes of refractory vomiting. We investigated the function of upper alimentary tract and found that esophago-gastric dysmotility and severe gastric volvulus were the major causes of gastrointestinal symptoms. This child was surgically treated with anterior gastropexy and jejunal alimentation through gastrostomy, and the symptoms were relieved with good weight gain. This report may provide insight into the gastrointestinal function and nutritional management in children with ATR-X syndrome. PMID:25252072

  2. Measured Thermal and Fast Neutron Fluence Rates for ATF-1 Holders During ATR Cycle 157D

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Larry Don [Idaho National Lab. (INL), Idaho Falls, ID (United States); Miller, David Torbet [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2016-03-01

    This report contains the thermal (2200 m/s) and fast (E>1MeV) neutron fluence rate data for the ATF-1 holders located in core for ATR Cycle 157D which were measured by the Radiation Measurements Laboratory (RML) as requested by the Power Reactor Programs (ATR Experiments) Radiation Measurements Work Order. This report contains measurements of the fluence rates corresponding to the particular elevations relative to the 80-ft. core elevation. The data in this report consist of (1) a table of the ATR power history and distribution, (2) a hard copy listing of all thermal and fast neutron fluence rates, and (3) plots of both the thermal and fast neutron fluence rates. The fluence rates reported are for the average power levels given in the table of power history and distribution.

  3. 10 CFR 830 Major Modification Determination for Replacement of ATR Primary Coolant Pumps and Motors

    Energy Technology Data Exchange (ETDEWEB)

    Noel Duckwitz

    2011-05-01

    The continued safe and reliable operation of the ATR is critical to the Department of Energy (DOE) Office of Nuclear Energy (NE) mission. While ATR is safely fulfilling current mission requirements, a variety of aging and obsolescence issues challenge ATR engineering and maintenance personnel’s capability to sustain ATR over the long term. First documented in a series of independent assessments, beginning with an OA Environmental Safety and Health Assessment conducted in 2003, the issues were validated in a detailed Material Condition Assessment (MCA) conducted as a part of the ATR Life Extension Program in 2007.Accordingly, near term replacement of aging and obsolescent original ATR equipment has become important to ensure ATR capability in support of NE’s long term national missions. To that end, a mission needs statement has been prepared for a non-major system acquisition which is comprised of three interdependent subprojects. The first project will replace the existent diesel-electrical bus (E-3), switchgear, and the 50-year-old obsolescent marine diesels with commercial power that is backed with safety related emergency diesel generators, switchgear, and uninterruptible power supply (UPS). The second project, the subject of this major modification determination, will replace the four, obsolete, original primary coolant pumps (PCPs) and motors. Completion of this and the two other age-related projects (replacement of the ATR diesel bus [E-3] and switchgear and replacement of the existent emergency firewater injection system) will resolve major age-related operational issues plus make a significant contribution in sustaining the ATR safety and reliability profile. The major modification criteria evaluation of the project pre-conceptual design identified several issues that lead to the conclusion that the project is a major modification: 1. Evaluation Criteria #3 (Change of existing process). The proposed strategy for equipping the replacement PCPs with VFDs

  4. 粉煤灰激活中ATR/FTIR、FTIR和NMR的分析与比较

    Institute of Scientific and Technical Information of China (English)

    易志慧; 蒋海青; 王晓钧; 于士刚

    2007-01-01

    采用ATR/FTIR对南京热电厂Ⅰ级粉煤灰及经过NaOH激发后的样品进行了分析,并且同FTIR和NMR的分析结果进行比较。研究结果表明:以表征表面特征的ATR/FTIR方法可以清晰地表征粉煤灰表面出现的变化,且随着碱浓度的提高变化明显,而以表征宏观几率特征的FTIR和NMR则没有反映出粉煤灰表面出现的变化。ATR/FTIR在粉煤灰的表面研究中具有独到效果。

  5. Modeling the attenuated total reflectance infrared (ATR-FTIR) spectrum of apatite

    Science.gov (United States)

    Aufort, Julie; Ségalen, Loïc; Gervais, Christel; Brouder, Christian; Balan, Etienne

    2016-06-01

    Attenuated total reflectance (ATR) infrared spectra were measured on a synthetic and a natural fluorapatite sample. A modeling approach based on the computation of the Fresnel reflection coefficient between the ATR crystal and the powder sample was used to analyze the line shape of the spectra. The dielectric properties of the samples were related to those of pure fluorapatite using an effective medium approach, based on Maxwell-Garnett and Bruggeman models. The Bruggeman effective medium model leads to a very good agreement with the experimental data recorded on the synthetic fluorapatite sample. The poorer agreement observed on the natural sample suggests a more significant heterogeneity of the sample at a characteristic length scale larger than the mid-infrared characteristic wavelength, i.e., about 10 micrometers. The results demonstrate the prominent role of macroscopic electrostatic effects over fine details of the microscopic structure in determining the line shape of strong ATR bands.

  6. Mutation analysis of the ATR gene in breast and ovarian cancer families

    International Nuclear Information System (INIS)

    Mutations in BRCA1, BRCA2, ATM, TP53, CHK2 and PTEN account for only 20–30% of the familial aggregation of breast cancer, which suggests the involvement of additional susceptibility genes. The ATR (ataxia-telangiectasia- and Rad3-related) kinase is essential for the maintenance of genomic integrity. It functions both in parallel and cooperatively with ATM, but whereas ATM is primarily activated by DNA double-strand breaks induced by ionizing radiation, ATR has been shown to respond to a much broader range of DNA damage. Upon activation, ATR phosphorylates several important tumor suppressors, including p53, BRCA1 and CHK1. Based on its central function in the DNA damage response, ATR is a plausible candidate gene for susceptibility to cancer. We screened the entire coding region of the ATR gene for mutations in affected index cases from 126 Finnish families with breast and/or ovarian cancer, 75 of which were classified as high-risk and 51 as moderate-risk families, by using conformation sensitive gel electrophoresis and direct sequencing. A large number of novel sequence variants were identified, four of which – Glu254Gly, Ser1142Gly, IVS24-48G>A and IVS26+15C>T – were absent from the tested control individuals (n = 300). However, the segregation of these mutations with the cancer phenotype could not be confirmed, partly because of the lack of suitable DNA samples. The present study does not support a major role for ATR mutations in hereditary susceptibility to breast and ovarian cancer

  7. Hubungan Kadar Ft4 Dengan Kejadian Tirotoksikosis berdasarkan Penilaian Indeks New Castle Padawanita Dewasa di Daerah Ekses Yodium

    Directory of Open Access Journals (Sweden)

    Harsa Rusda

    2013-05-01

    Full Text Available AbstrakTirotoksikosis merupakan manifestasi klinis yang terjadi akibat peningkatan kadar hormon tiroid dalam darah. Kelebihan yodium merupakan salah satu penyebab terjadinya tirotoksikosis. Ini ditandai dengan hasil pemeriksaan kadar Ekskresi Yodium Urin (EYU > 199 μg/L. Tujuan: Penelitian ini bertujuan untuk mengetahui hubungan kadar FT4 dengan kejadian tirotoksikosis berdasarkan penilaian indeks New Castle pada wanita dewasa di daerah ekses yodium. Metode: Penelitian dilakukan dengan menganalisis data yang dikumpulkan secara Cross Sectional Study terhadap 37 wanita dewasa menggunakan metoda total sampling di Nagari Koto Salak, Kecamatan Koto Salak Kabupaten Dharmasraya yang merupakan daerah ekses yodium (median EYU 323,5 μg/L. Pengumpulan data dilakukan dengan wawancara menggunakan penilaian Indeks New Castle dan pengambilan darah untuk dilakukan pemeriksaan kadar FT4 dalam serum. Hasil: Analisis univariat didapatkan jumlah penduduk wanita dewasa dengan kadar FT4 meningkat sebanyak 14 persen dan nilai rata-rata 1,71 ng/dl. Penilaian indeks New Castle dalam kategori doubtful 16 persen dan tidak ditemukan penduduk yang termasuk dalam kategori toxic. Berdasarkan hasil uji statistik Chi-square, didapatkan p value=1. Kesimpulan: Tidak terdapat hubungan yang bermakna antara kadar FT4 dalam serum dengan kejadian tirotoksikosis pada wanita dewasa di derah ekses yodium. Saran: Perlu dilakukan penyuluhan mengenai asupan yodium kepada masyarakat dan diharapkan penelitian ini dapat menjadi masukan kepada pemerintah dalam mengambil kebijakan terhadap pemberian kapsul yodium serta melakukan kontrol kadar EYU secara teratur dan berkala.Kata kunci: FT4, tirotoksikosis, indeks New Castle, wanita, ekses yodium, tiroidAbstractThyrotoxicosis is a clinical manifestation that occurs due to elevated levels of thyroid hormones in the blood. Iodine excess is one of the causes of thyrotoxicosis. This is indicated by the results of urine iodine excretion levels (EYU

  8. ATR-FTIR Spectroscopy in the Undergraduate Chemistry Laboratory: Part II--A Physical Chemistry Laboratory Experiment on Surface Adsorption

    Science.gov (United States)

    Schuttlefield, Jennifer D.; Larsen, Sarah C.; Grassian, Vicki H.

    2008-01-01

    Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy is a useful technique for measuring the infrared spectra of solids and liquids as well as probing adsorption on particle surfaces. The use of FTIR-ATR spectroscopy in organic and inorganic chemistry laboratory courses as well as in undergraduate research was presented…

  9. Effects of atrazine (ATR), deisopropylatrazine (DIA), Diaminochlorotriazine (DACT) on the hypothalamic-pituitary-adrenal (HPA) axis in female rats

    Science.gov (United States)

    We previously reported that a single dose of the herbicide ATR stimulated the HPA axis in the male rat while equimolar doses of its primary metabolite, DACT, had a minimal effect. In this study, we evaluated the effects of one or four daily doses of ATR, DACT, and an intermediat...

  10. EFFECTS OF ATRAZINE (ATR), DEISOPROPYLATRAZINE (DIA), AND DIAMINOCHLOROTRIAZINE (DACT) ON THE HYPOTHALAMIC-PITUITARY-ADRENAL (HPA) AXIS IN FEMALE RATS

    Science.gov (United States)

    Previously we reported that a single dose of ATR herbicide stimulated HPA axis activation in the male rat while its primary metabolite, DACT, did so to a lesser extent. In this study, we evaluated the effects of ATR, DACT, and an intermediate metabolite, DIA, on adrenocorticotrop...

  11. Radar Sensor Networks: Algorithms for Waveform Design and Diversity with Application to ATR with Delay-Doppler Uncertainty

    Directory of Open Access Journals (Sweden)

    Qilian Liang

    2007-01-01

    Full Text Available Automatic target recognition (ATR in target search phase is very challenging because the target range and mobility are not yet perfectly known, which results in delay-Doppler uncertainty. In this paper, we firstly perform some theoretical studies on radar sensor network (RSN design based on linear frequency modulation (LFM waveform: (1 the conditions for waveform coexistence, (2 interferences among waveforms in RSN, (3 waveform diversity in RSN. Then we apply RSN to ATR with delay-Doppler uncertainty and propose maximum-likeihood (ML ATR algorithms for fluctuating targets and nonfluctuating targets. Simulation results show that our RSN vastly reduces the ATR error compared to a single radar system in ATR with delay-Doppler uncertainty. The proposed waveform design and diversity algorithms can also be applied to active RFID sensor networks and underwater acoustic sensor networks.

  12. S-phase-dependent p50/NF-кB1 phosphorylation in response to ATR and replication stress acts to maintain genomic stability

    OpenAIRE

    Crawley, Clayton D.; Kang, Shijun; Bernal, Giovanna M; Wahlstrom, Joshua S.; Voce, David J; Cahill, Kirk E.; Garofalo, Andrea; Raleigh, David R.; Weichselbaum, Ralph R.; Yamini, Bakhtiar

    2015-01-01

    The apical damage kinase, ATR, is activated by replication stress (RS) both in response to DNA damage and during normal S-phase. Loss of function studies indicates that ATR acts to stabilize replication forks, block cell cycle progression and promote replication restart. Although checkpoint failure and replication fork collapse can result in cell death, no direct cytotoxic pathway downstream of ATR has previously been described. Here, we show that ATR directly reduces survival by inducing pho...

  13. FTIR-ATR Monitoring and SEC/RI/MALLS characterization of ATRP synthesized hyperbranched polyacrylates

    OpenAIRE

    Gonçalves, Miguel; Costa, Mário Rui; Dias, Rolando

    2009-01-01

    This work reports the synthesis at 1 L scale of hyperbranched polyacrylates based upon acrylate/diacrylate monomers such as n-butyl acrylate (nBA)/1,6-hexanediol diacrylate (HDDA) and using atom transfer radical polymerization (ATRP). A FTIR-ATR immersion probe was used to monitor the polymerization reaction. FCT

  14. Multivariate analysis of ATR-FTIR spectra for assessment of oil shale organic geochemical properties

    Science.gov (United States)

    Washburn, Kathryn E.; Birdwell, Justin E.

    2013-01-01

    In this study, attenuated total reflectance (ATR) Fourier transform infrared spectroscopy (FTIR) was coupled with partial least squares regression (PLSR) analysis to relate spectral data to parameters from total organic carbon (TOC) analysis and programmed pyrolysis to assess the feasibility of developing predictive models to estimate important organic geochemical parameters. The advantage of ATR-FTIR over traditional analytical methods is that source rocks can be analyzed in the laboratory or field in seconds, facilitating more rapid and thorough screening than would be possible using other tools. ATR-FTIR spectra, TOC concentrations and Rock–Eval parameters were measured for a set of oil shales from deposits around the world and several pyrolyzed oil shale samples. PLSR models were developed to predict the measured geochemical parameters from infrared spectra. Application of the resulting models to a set of test spectra excluded from the training set generated accurate predictions of TOC and most Rock–Eval parameters. The critical region of the infrared spectrum for assessing S1, S2, Hydrogen Index and TOC consisted of aliphatic organic moieties (2800–3000 cm−1) and the models generated a better correlation with measured values of TOC and S2 than did integrated aliphatic peak areas. The results suggest that combining ATR-FTIR with PLSR is a reliable approach for estimating useful geochemical parameters of oil shales that is faster and requires less sample preparation than current screening methods.

  15. An add-on system including a micro-reactor for an atr-ir spectrometer

    DEFF Research Database (Denmark)

    2014-01-01

    The invention relates to an add-on system for an attenuated total reflectance infrared (ATR-IR) spectrometer, the add-on system allowing for time-resolved in situ IR measurements of heterogeneous mixtures. The add-on device comprises a micro-reactor (300A) forming a sample cavity (305) when...

  16. MATERNAL ATRAZINE (ATR) ALTERS HYPOTHALAMIC DOPAMINE (HYP-DA) AND SERUM PROLACTIN (SPRL) IN MALE PUPS

    Science.gov (United States)

    Maternal Atrazine (ATR) alters hypothalamic dopamine (HYP-DA) and serum prolactin (sPRL) in male pups. 1Christopher Langdale, 2Tammy Stoker and 2Ralph Cooper. 1 Dept. of Cell Biology, North Carolina State University College of Veterinary Medicine, Raleigh, NC. 2 Endocrinology ...

  17. Solid Acid-Catalyzed Cellulose Hydrolysis Monitored by In Situ ATR-IR Spectroscopy

    NARCIS (Netherlands)

    Zakzeski, J.; Grisel, R.J.H.; Smit, A.T.; Weckhuysen, B.M.

    2012-01-01

    The solid acid-catalyzed hydrolysis of cellulose was studied under elevated temperatures and autogenous pressures using in situ ATR-IR spectroscopy. Standards of cellulose and pure reaction products, which include glucose, fructose, hydroxymethylfurfural (HMF), levulinic acid (LA), formic acid, and

  18. Fabrication Report for the AFC-2A and AFC-2B Capsule Irradiations in the ATR

    Energy Technology Data Exchange (ETDEWEB)

    Timothy A. Hyde

    2007-10-01

    This document provides a general narrative description of the AFC-2A and 2B fuel fabrication processes for the AFC 2A and AFC 2B fuel irradiation experiments fabricated at the Idaho National Laboratory’s Materials and Fuels Complex (MFC) for irradiation in the Advanced Test Reactor (ATR).

  19. VE-821, an ATR inhibitor, causes radiosensitization in human tumor cells irradiated with high LET radiation

    International Nuclear Information System (INIS)

    High linear energy transfer (LET) radiation such as carbon ion particles is successfully used for treatment of solid tumors. The reason why high LET radiation accomplishes greater tumor-killing than X-rays is still not completely understood. One factor would be the clustered or complex-type DNA damages. We previously reported that complex DNA double-strand breaks produced by high LET radiation enhanced DNA end resection, and this could lead to higher kinase activity of ATR protein recruited to RPA-coated single-stranded DNA. Although the effect of ATR inhibition on cells exposed to low LET gamma-rays has recently been reported, little is known regarding the effect of ATR inhibitor on cells treated with high LET radiation. The purpose of this study is to investigate the effects of the ATR inhibitor VE-821 in human tumor and normal cells irradiated with high LET carbon ions. HeLa, U2OS, and 1BR-hTERT (normal) cells were pre-treated with 1 μM VE-821 for 1 hour and irradiated with either high LET carbon ions or X-rays. Cell survival, cell cycle distribution, cell growth, and micronuclei formation were evaluated. VE-821 caused abrogation of G2/M checkpoint and forced irradiated cells to divide into daughter cells. We also found that carbon ions caused a higher number of multiple micronuclei than X-rays, leading to decreased cell survival in tumor cells when treated with VE-821, while the survival of irradiated normal cells were not significantly affected by this inhibitor. ATR inhibitor would be an effective tumor radiosensitizer with carbon ion irradiation. The online version of this article (doi:10.1186/s13014-015-0464-y) contains supplementary material, which is available to authorized users

  20. Protein phosphatase 5 is necessary for ATR-mediated DNA repair

    International Nuclear Information System (INIS)

    Research highlights: → Serine/threonine protein phosphatase 5 (PP5) has been shown to participate in ataxia telangiectasia-mutated (ATM)- and ATR (ATM- and Rad3-related)-mediated checkpoint pathways, which plays an important role in the DNA damage response and maintenance of genomic stability. → However, it is not clear exactly how PP5 participates in this process. → Our results indicate that PP5 is more closely related with ATR-mediated pathway than ATM-mediated pathway in DNA damage repair. -- Abstract: Several recent studies have shown that protein phosphatase 5 (PP5) participates in cell cycle arrest after DNA damage, but its roles in DNA repair have not yet been fully characterized. We investigated the roles of PP5 in the repair of ultraviolet (UV)- and neocarzinostatin (NCS)-induced DNA damage. The results of comet assays revealed different repair patterns in UV- and NCS-exposed U2OS-PS cells. PP5 is only essential for Rad3-related (ATR)-mediated DNA repair. Furthermore, the phosphorylation of 53BP1 and BRCA1, important mediators of DNA damage repair, and substrates of ATR and ATM decreased in U2OS-PS cells exposed to UV radiation. In contrast, the cell cycle arrest proteins p53, CHK1, and CHK2 were normally phosphorylated in U2OS and U2OS-PS cells exposed to UV radiation or treated with NCS. In view of these results, we suggest that PP5 plays a crucial role in ATR-mediated repair of UV-induced DNA damage.

  1. Protein phosphatase 5 is necessary for ATR-mediated DNA repair

    Energy Technology Data Exchange (ETDEWEB)

    Kang, Yoonsung [Department of Pharmacology, DNA Repair Research Center, Chosun University School of Medicine, 375 Seosuk-Dong, Gwangju 501-759 (Korea, Republic of); Cheong, Hyang-Min [Department of Life Science, College of Natural Science, Chung-Ang University, 221 Heuksuk-Dong, Dongjak-Ku, Seoul 156-756 (Korea, Republic of); Lee, Jung-Hee [Department of Pharmacology, DNA Repair Research Center, Chosun University School of Medicine, 375 Seosuk-Dong, Gwangju 501-759 (Korea, Republic of); Song, Peter I. [Department of Dermatology, University of Arkansas for Medical Science, 4301 West Markham, Slot 576, Little Rock, AR 72205 (Korea, Republic of); Lee, Kwang-Ho [Department of Life Science, College of Natural Science, Chung-Ang University, 221 Heuksuk-Dong, Dongjak-Ku, Seoul 156-756 (Korea, Republic of); Kim, Sang-Yong [Division of Endocrinology, Department of Internal Medicine, Chosun University School of Medicine, 375 Seosuk-Dong, Gwangju 501-759 (Korea, Republic of); Jun, Jae Yeoul [Department of Physiology, Chosun University School of Medicine, 375 Seosuk-Dong, Gwangju 501-759 (Korea, Republic of); You, Ho Jin, E-mail: hjyou@chosun.ac.kr [Department of Pharmacology, DNA Repair Research Center, Chosun University School of Medicine, 375 Seosuk-Dong, Gwangju 501-759 (Korea, Republic of)

    2011-01-07

    Research highlights: {yields} Serine/threonine protein phosphatase 5 (PP5) has been shown to participate in ataxia telangiectasia-mutated (ATM)- and ATR (ATM- and Rad3-related)-mediated checkpoint pathways, which plays an important role in the DNA damage response and maintenance of genomic stability. {yields} However, it is not clear exactly how PP5 participates in this process. {yields} Our results indicate that PP5 is more closely related with ATR-mediated pathway than ATM-mediated pathway in DNA damage repair. -- Abstract: Several recent studies have shown that protein phosphatase 5 (PP5) participates in cell cycle arrest after DNA damage, but its roles in DNA repair have not yet been fully characterized. We investigated the roles of PP5 in the repair of ultraviolet (UV)- and neocarzinostatin (NCS)-induced DNA damage. The results of comet assays revealed different repair patterns in UV- and NCS-exposed U2OS-PS cells. PP5 is only essential for Rad3-related (ATR)-mediated DNA repair. Furthermore, the phosphorylation of 53BP1 and BRCA1, important mediators of DNA damage repair, and substrates of ATR and ATM decreased in U2OS-PS cells exposed to UV radiation. In contrast, the cell cycle arrest proteins p53, CHK1, and CHK2 were normally phosphorylated in U2OS and U2OS-PS cells exposed to UV radiation or treated with NCS. In view of these results, we suggest that PP5 plays a crucial role in ATR-mediated repair of UV-induced DNA damage.

  2. The ABC transporter ATR1 is necessary for efflux of the toxin cercosporin in the fungus Cercospora nicotianae.

    Science.gov (United States)

    Amnuaykanjanasin, Alongkorn; Daub, Margaret E

    2009-02-01

    The Cercospora nicotianae mutant deficient for the CRG1 transcription factor has marked reductions in both resistance and biosynthesis of the toxin cercosporin. We cloned and sequenced full-length copies of two genes, ATR1 and CnCFP, previously identified from a subtractive library between the wild type (WT) and a crg1 mutant. ATR1 is an ABC transporter gene and has an open reading frame (ORF) of 4368bp with one intron. CnCFP encodes a MFS transporter with homology to Cercospora kikuchii CFP, previously implicated in cercosporin export, and has an ORF of 1975bp with three introns. Disruption of ATR1 indicated atr1-null mutants had dramatic reductions in cercosporin production (25% and 20% of WT levels) in solid and liquid cultures, respectively. The ATR1 disruptants also showed moderately higher sensitivity to cercosporin. Constitutive expression of ATR1 in the crg1 mutant restored cercosporin biosynthesis and moderately increased resistance. In contrast, CnCFP overexpression in the mutant did not restore toxin production, however, it moderately enhanced toxin resistance. The results together indicate ATR1 acts as a cercosporin efflux pump in this fungus and plays a partial role in resistance.

  3. Advanced Test Reactor National Scientific User Facility (ATR NSUF) Monthly Report November 2014

    Energy Technology Data Exchange (ETDEWEB)

    Soelberg, Renae [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2014-11-01

    Advanced Test Reactor National Scientific User Facility (ATR NSUF) Monthly Report November 2014 Highlights Rory Kennedy and Sarah Robertson attended the American Nuclear Society Winter Meeting and Nuclear Technology Expo in Anaheim, California, Nov. 10-13. ATR NSUF exhibited at the technology expo where hundreds of meeting participants had an opportunity to learn more about ATR NSUF. Dr. Kennedy briefed the Nuclear Engineering Department Heads Organization (NEDHO) on the workings of the ATR NSUF. • Rory Kennedy, James Cole and Dan Ogden participated in a reactor instrumentation discussion with Jean-Francois Villard and Christopher Destouches of CEA and several members of the INL staff. • ATR NSUF received approval from the NE-20 office to start planning the annual Users Meeting. The meeting will be held at INL, June 22-25. • Mike Worley, director of the Office of Innovative Nuclear Research (NE-42), visited INL Nov. 4-5. Milestones Completed • Recommendations for the Summer Rapid Turnaround Experiment awards were submitted to DOE-HQ Nov. 12 (Level 2 milestone due Nov. 30). Major Accomplishments/Activities • The University of California, Santa Barbara 2 experiment was unloaded from the GE-2000 at HFEF. The experiment specimen packs will be removed and shipped to ORNL for PIE. • The Terrani experiment, one of three FY 2014 new awards, was completed utilizing the Advanced Photon Source MRCAT beamline. The experiment investigated the chemical state of Ag and Pd in SiC shell of irradiated TRISO particles via X-ray Absorption Fine Structure (XAFS) spectroscopy. Upcoming Meetings/Events • The ATR NSUF program review meeting will be held Dec. 9-10 at L’Enfant Plaza. In addition to NSUF staff and users, NE-4, NE-5 and NE-7 representatives will attend the meeting. Awarded Research Projects Boise State University Rapid Turnaround Experiments (14-485 and 14-486) Nanoindentation and TEM work on the T91, HT9, HCM12A and 9Cr ODS specimens has been completed at

  4. Coupling of phonon-polariton modes at dielectric-dielectric interfaces by the ATR technique

    Science.gov (United States)

    Cocoletzi, G. H.; Olvera Hernández, J.; Martínez Montes, G.

    1989-08-01

    We report the calculated ATR dispersion relation of the interface phonon-polariton modes in the prism-dielectric-dielectric configuration. Comparison of electromagnetic dispersion relations (EMDR) with the ATR dispersion relations are presented for three different interfaces: I) GaAs/GaP, II) CdF2/CaF2 and III) CaF2/GaP in two propagation windows, using the Otto and Kretschmann geometries for p-polarized light. We have studied the three cases using angle and frequency scans for each window and geometry. The results indicate that it is possible to excite and detect phonon-polariton modes at the dielectric-dielectric interface.

  5. ATR-A1 irradiation experiment on vanadium alloys and low activation steels

    Energy Technology Data Exchange (ETDEWEB)

    Tasi, H.; Strain, R.V.; Gomes, I.; Hins, A.G.; Smith, D.L.

    1996-04-01

    To study the mechanical properties of vanadium alloys under neutron irradiation at low temperatures, an experiment was designed and constructed for irradiation in the Advanced Test Reactor (ATR). The experiment contained Charpy, tensile, compact tension, TEM, and creep specimens of vanadium alloys. It also contained limited low-activation ferritic steel specimens as part of the collaborative agreement with Monbusho of Japan. The design irradiation temperatures for the vanadium alloy specimens in the experiment are {approx}200 and 300{degrees}C, achieved with passive gap-gap sizing and fill gas blending. To mitigate vanadium-to-chromium transmutation from the thermal neutron flux, the test specimens are contained inside gadolinium flux filters. All specimens are lithium-bonded. The irradiation started in Cycle 108A (December 3, 1995) and is expected to have a duration of three ATR cycles and a peak influence of 4.4 dpa.

  6. Optimization of Adaboost Algorithm for Sonar Target Detection in a Multi-Stage ATR System

    Science.gov (United States)

    Lin, Tsung Han (Hank)

    2011-01-01

    JPL has developed a multi-stage Automated Target Recognition (ATR) system to locate objects in images. First, input images are preprocessed and sent to a Grayscale Optical Correlator (GOC) filter to identify possible regions-of-interest (ROIs). Second, feature extraction operations are performed using Texton filters and Principal Component Analysis (PCA). Finally, the features are fed to a classifier, to identify ROIs that contain the targets. Previous work used the Feed-forward Back-propagation Neural Network for classification. In this project we investigate a version of Adaboost as a classifier for comparison. The version we used is known as GentleBoost. We used the boosted decision tree as the weak classifier. We have tested our ATR system against real-world sonar images using the Adaboost approach. Results indicate an improvement in performance over a single Neural Network design.

  7. Phylogeny of cultivated and wild wheat species using ATR-FTIR spectroscopy

    Science.gov (United States)

    Demir, Pinar; Onde, Sertac; Severcan, Feride

    2015-01-01

    Within the last decade, an increasing amount of genetic data has been used to clarify the problems inherent in wheat taxonomy. The techniques for obtaining and analyzing these data are not only cumbersome, but also expensive and technically demanding. In the present study, we introduce infrared spectroscopy as a method for a sensitive, rapid and low cost phylogenetic analysis tool for wheat seed samples. For this purpose, 12 Triticum and Aegilops species were studied by Attenuated Total Reflection-Fourier Transform Infrared (ATR-FTIR) spectroscopy. Hierarchical cluster analysis and principal component analysis clearly revealed that the lignin band (1525-1505 cm-1) discriminated the species at the genus level. However, the species were clustered according to their genome commonalities when the whole spectra were used (4000-650 cm-1). The successful differentiation of Triticum and its closely related genus Aegilops clearly demonstrated the power of ATR-FTIR spectroscopy as a suitable tool for phylogenetic research.

  8. Enhanced ATR algorithm for high resolution multi-band sonar imagery

    Science.gov (United States)

    Aridgides, Tom; Fernández, Manuel

    2008-04-01

    An improved automatic target recognition (ATR) processing string has been developed. The overall processing string consists of pre-processing, subimage adaptive clutter filtering (SACF), normalization, detection, data regularization, feature extraction, optimal subset feature selection, feature orthogonalization and classification processing blocks. A new improvement was made to the processing string, data regularization, which entails computing the input data mean, clipping the data to a multiple of its mean and scaling it, prior to feature extraction. The classified objects of 3 distinct strings are fused using the classification confidence values and their expansions as features, and using "summing" or log-likelihood-ratio-test (LLRT) based fusion rules. The utility of the overall processing strings and their fusion was demonstrated with new high-resolution three-frequency band sonar imagery. The ATR processing strings were individually tuned to the corresponding three-frequency band data, making use of the new processing improvement, data regularization, which resulted in a 3:1 reduction in false alarms. Two significant fusion algorithm improvements were made. First, a nonlinear 2nd order (Volterra) feature LLRT fusion algorithm was developed. Second, a repeated application of a subset Volterra feature selection / feature orthogonalization / LLRT fusion block was utilized. It was shown that cascaded Volterra feature LLRT fusion of the ATR processing strings outperforms baseline summing and single-stage Volterra feature LLRT algorithms, yielding significant improvements over the best single ATR processing string results, and providing the capability to correctly call the majority of targets while maintaining a very low false alarm rate.

  9. The Conference Proceedings of the 2003 Air Transport Research Society (ATRS) World Conference, Volume 3

    Science.gov (United States)

    Bowen, Brent (Editor); Gudmundsson, Sveinn (Editor); Oum, Tae (Editor)

    2003-01-01

    Volume 3 of the 2003 Air Transport Reserch Society (ATRS) World Conference includes papers on topics relevant to airline operations worldwide. Specific topics include: European Union and civil aviation regimens;simulating decision making in airline operations, passenger points of view on convenient airports; route monopolies and nonlinear pricing; cooperation among airports in Europe; fleet modernizaiton in Brazil;the effects of deregulation on the growth of air transportation in Europe and the United States.

  10. An Approach for Routine Analytical Detection of Beeswax Adulteration Using FTIR-ATR Spectroscopy

    OpenAIRE

    Svečnjak Lidija; Baranović Goran; Vinceković Marko; Prđun Saša; Bubalo Dragan; Tlak Gajger Ivana

    2015-01-01

    Although beeswax adulteration represents one of the main beeswax quality issues, there are still no internationally standardised analytical methods for routine quality control. The objective of this study was to establish an analytical procedure suitable for routine detection of beeswax adulteration using FTIR-ATR spectroscopy. For the purpose of this study, reference IR spectra of virgin beeswax, paraffin, and their mixtures containing different proportions of paraffin (5 - 95%), were obtain...

  11. Gauvain e il corpo smembrato: allegorie nell'«Atre périlleux»

    Directory of Open Access Journals (Sweden)

    Maurizio Virdis

    2013-07-01

    Full Text Available L’Atre Périlleux è un romanzo anonimo francese del XIII secolo e fa parte della corrente letteraria che rimettere in questione il romanzo cortese di tradizione chrestieniana. Piú che all’ideologia o alle istanze della classe cavalleresca e della nobiltà minore, il nostro romanzo risponde alle prospettive e alle necessità intellettuali della clergie, che si fa gioco dei valori cavallereschi tradizionali. L’Atre Périlleux è condotto, con toni di humour e una trama complessa, su binari metaletterari e allegorici (di cui il corpo smembrato di Gauvain è il simbolo piú eminente, e si pone come critica della decadenza della società cortese, animato però dal proposito del rinnovamento morale di essa.L’Atre Périlleux is an anonymous French romance of the thirteenth century and it is part of the literary movement that calls into question the tradition of courtly romance dating back to Chrétien de Troyes. More than to the ideology and instances of the class of minor nobility and chivalry, our romance responds to the clergie’s needs and intellectual perspectives, and it mocks the traditional values of chivalry. L’Atre Périlleux is conducted, with a hint of humour and a complicated plot, on metaliterary and allegorical tracks (and Gauvain’s dismembered body is the most prominent symbol of this allegorical tone, so that our romance stands as a critique of courtly society decadence, nevertheless animated by the purpose of its moral renewal.

  12. Hardening neutron spectrum for advanced actinide transmutation experiments in the ATR.

    Science.gov (United States)

    Chang, G S; Ambrosek, R G

    2005-01-01

    The most effective method for transmuting long-lived isotopes contained in spent nuclear fuel into shorter-lived fission products is in a fast neutron spectrum reactor. In the absence of a fast test reactor in the United States, initial irradiation testing of candidate fuels can be performed in a thermal test reactor that has been modified to produce a test region with a hardened neutron spectrum. Such a test facility, with a spectrum similar but somewhat softer than that of the liquid-metal fast breeder reactor (LMFBR), has been constructed in the INEEL's Advanced Test Reactor (ATR). The radial fission power distribution of the actinide fuel pin, which is an important parameter in fission gas release modelling, needs to be accurately predicted and the hardened neutron spectrum in the ATR and the LMFBR fast neutron spectrum is compared. The comparison analyses in this study are performed using MCWO, a well-developed tool that couples the Monte Carlo transport code MCNP with the isotope depletion and build-up code ORIGEN-2. MCWO analysis yields time-dependent and neutron-spectrum-dependent minor actinide and Pu concentrations and detailed radial fission power profile calculations for a typical fast reactor (LMFBR) neutron spectrum and the hardened neutron spectrum test region in the ATR. The MCWO-calculated results indicate that the cadmium basket used in the advanced fuel test assembly in the ATR can effectively depress the linear heat generation rate in the experimental fuels and harden the neutron spectrum in the test region.

  13. ATR-16 due to a de novo complex rearrangement of chromosome 16.

    Science.gov (United States)

    Gallego, Marta S; Zelaya, Gabriela; Feliu, Aurora S; Rossetti, Liliana; Shaffer, Lisa G; Bailey, Kristen A; Bacino, Carlos A; Barreiro, Cristina Z

    2005-01-01

    We describe a child with ATR-16 [alpha-thalassemia (thal)/mental retardation], who was referred for genetic evaluation because of minor anomalies and developmental delay. Cytogenetic analysis demonstrated a de novo complex rearrangement of chromosome 16. Fluorescence in situ hybridization (FISH) analysis, using chromosome 16 subtelomeric probes, showed that this patient had a deletion of the distal short arm of chromosome 16 that contains the alpha-globin genes and a duplication of 16q. Analysis of the alpha-globin locus by Southern blot showed a half normal dose of the alpha-globin gene. Microsatellite marker studies revealed that the duplicated 16q region was maternal in origin. Hematological studies revealed anemia, hypochromia and occasional cells with Hb H inclusion bodies. A hematological screening for alpha-thal should be considered in patients with mild developmental delay and a suggestive phenotype of ATR-16 with microcytic hypochromic anemia and normal iron status. The stellate pattern of the iris, a new finding in our patient, may contribute to a better clinical delineation of both syndromes, ATR-16 and/or duplication of 16qter.

  14. ATR-IR Investigation of Solvent Interactions with Surface-Bound Polymers

    Science.gov (United States)

    2016-01-01

    Solvent interactions with bulk and surface-bound polymer brushes are crucial for functionalities such as controlled friction and thermoresponsive adhesion. To study such interactions, the temperature-induced solvent-quality changes and the effect of surface tethering on the mechanical and tribological properties of poly(dodecyl methacrylate) (P12MA) brushes have been investigated by means of attenuated total reflection infrared spectroscopy (ATR-IR), as well as atomic force microscopy (AFM) and lateral force microscopy (LFM). These results have been compared with temperature-dependent UV–visible spectrophotometry (UV–vis) data for the corresponding bulk polymer solutions. The ATR-IR results clearly show that increasing temperature enhances ethanol uptake in P12MA, which results in film swelling. This is accompanied by a marked increase in both adhesion and friction. We have also shown that a combination of solvents, such as toluene and ethanol, can lead to a temperature-dependent solvent partitioning within the polymer brush. To our knowledge this is the first time preferential solvent uptake in a grafted-from brush has been monitored via in situ ATR-IR. Moreover, we have observed remarkably different behavior for polymer chains in solution compared to the behavior of similar chains bound to a surface. The presented findings on the temperature-dependent solvent interactions of surface-grafted P12MA reveal previously unknown solvation phenomena and open up a range of possible applications in the area of stimuli-responsive materials. PMID:27397856

  15. Cyclohexene Photo-oxidation over Vanadia Catalyst Analyzed by Time Resolved ATR-FT-IR Spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Frei, Heinz; Mul, Guido; Wasylenko, Walter; Hamdy, M. Sameh; Frei, Heinz

    2008-06-04

    Vanadia was incorporated in the 3-dimensional mesoporous material TUD-1 with a loading of 2percent w/w vanadia. The performance in the selective photo-oxidation of liquid cyclohexene was investigated using ATR-FT-IR spectroscopy. Under continuous illumination at 458 nm a significant amount of product, i.e. cyclohexenone, was identified. This demonstrates for the first time that hydroxylated vanadia centers in mesoporous materials can be activated by visible light to induce oxidation reactions. Using the rapid scan method, a strong perturbation of the vanadyl environment could be observed in the selective oxidation process induced by a 458 nm laser pulse of 480 ms duration. This is proposed to be caused by interaction of the catalytic centre with a cyclohexenyl hydroperoxide intermediate. The restoration of the vanadyl environment could be kinetically correlated to the rate of formation of cyclohexenone, and is explained by molecular rearrangement and dissociation of the peroxide to ketone and water. The ketone diffuses away from the active center and ATR infrared probing zone, resulting in a decreasing ketone signal on the tens of seconds time scale after initiation of the photoreaction. This study demonstrates the high potential of time resolved ATR FT-IR spectroscopy for mechanistic studies of liquid phase reactions by monitoring not only intermediates and products, but by correlating the temporal behavior of these species to molecular changes of the vanadyl catalytic site.

  16. FTIR-ATR spectroscopy applied to quality control of grape-derived spirits.

    Science.gov (United States)

    Anjos, Ofélia; Santos, António J A; Estevinho, Letícia M; Caldeira, Ilda

    2016-08-15

    The Fourier transform infrared (FTIR) spectroscopic method with attenuated total reflectance (ATR) was used for predicting the alcoholic strength, the methanol, acetaldehyde and fusel alcohols content of grape-derived spirits. FTIR-ATR spectrum in the mid-IR region (4000-400cm(-1)) was used for the quantitative estimation by applying partial least square (PLS) regression models and the results were correlated with those obtained from reference methods. In the developed method, a cross-validation with 50% of the samples was used for PLS analysis along with a validation test set with 50% of the remaining samples. Good correlation models with a great accuracy were obtained for methanol (r(2)=99.4; RPD=12.8), alcoholic strength (r(2)=97.2; RPD=6.0), acetaldehyde (r(2)=98.2; RPD=7.5) and fusel alcohols (r(2) from 97.4 to 94.1; RPD from 6.2 to 4.1). These results corroborate the hypothesis that FTIR-ATR is a useful technique for the quality control of grape-derived spirits, whose practical application may improve the efficiency and quickness of the current laboratory analysis. PMID:27006210

  17. Submission of FeCrAl Feedstock for Support of AFC ATR-2 Irradiations

    Energy Technology Data Exchange (ETDEWEB)

    Field, Kevin G. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Barrett, Kristine E. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Sun, Zhiqian [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Yamamoto, Yukinori [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2016-09-16

    The Advanced Test Reactor (ATR) is currently being used to test accident tolerant fuel (ATF) forms destined for commercial nuclear power plant deployment. One irradiation program using the ATR for ATF concepts, Accident Tolerant Fuel-2 (ATF-2), is a water loop irradiation test using miniaturized fuel pins as test articles. This complicated testing configuration requires a series of pre-test experiments and verification including a flowing loop autoclave test and a sensor qualification test (SQT) prior to full test train deployment within the ATR. In support of the ATF-2 irradiation program, Oak Ridge National Laboratory (ORNL) has supplied two different Generation II FeCrAl alloys in rod stock form to Idaho National Laboratory (INL). These rods will be machined into dummy pins for deployment in the autoclave test and SQT. Post-test analysis of the dummy pins will provide initial insight into the performance of Generation II FeCrAl alloys in the ATF-2 irradiation experiment as well as within a commercial nuclear reactor.

  18. Submission of FeCrAl feedstock for support of AFC ATR-2 Irradiations

    Energy Technology Data Exchange (ETDEWEB)

    Field, Kevin G [ORNL; Barrett, Kristine E. [Idaho National Laboratory (INL); Sun, Zhiqian [ORNL; Yamamoto, Yukinori [ORNL

    2016-09-01

    The Advanced Test Reactor (ATR) is currently being used to test accident tolerant fuel (ATF) forms destined for commercial nuclear power plant deployment. One irradiation program using the ATR for ATF concepts, Accident Tolerant Fuel-2 (ATF-2), is a water loop irradiation test using miniaturized fuel pins as test articles. This complicated testing configuration requires a series of pre-test experiments and verification including a flowing loop autoclave test and a sensor qualification test (SQT) prior to full test train deployment within the ATR. In support of the ATF-2 irradiation program, Oak Ridge National Laboratory (ORNL) has supplied two different Generation II FeCrAl alloys in rod stock form to Idaho National Laboratory (INL). These rods will be machined into dummy pins for deployment in the autoclave test and SQT. Post-test analysis of the dummy pins will provide initial insight into the performance of Generation II FeCrAl alloys in the ATF-2 irradiation experiment as well as within a commercial nuclear reactor.

  19. Diamond-coated ATR prism for infrared absorption spectroscopy of surface-modified diamond nanoparticles

    International Nuclear Information System (INIS)

    Linear antenna microwave chemical vapor deposition process was used to homogeneously coat a 7 cm long silicon prism by 85 nm thin nanocrystalline diamond (NCD) layer. To show the advantages of the NCD-coated prism for attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) of nanoparticles, we apply diamond nanoparticles (DNPs) of 5 nm nominal size with various surface modifications by a drop-casting of their methanol dispersions. ATR-FTIR spectra of as-received, air-annealed, plasma-oxidized, and plasma-hydrogenated DNPs were measured in the 4000–1500 cm−1 spectral range. The spectra show high spectral resolution, high sensitivity to specific DNP surface moieties, and repeatability. The NCD coating provides mechanical protection against scratching and chemical stability of the surface. Moreover, unlike on bare Si surface, NCD hydrophilic properties enable optically homogeneous coverage by DNPs with some aggregation on submicron scale as evidenced by scanning electron microscopy and atomic force microscopy. Compared to transmission FTIR regime with KBr pellets, direct and uniform deposition of DNPs on NCD-ATR prism significantly simplifies and speeds up the analysis (from days to minutes). We discuss prospects for in situ monitoring of surface modifications and molecular grafting.

  20. The MRE11 GAR motif regulates DNA double-strand break processing and ATR activation

    Institute of Scientific and Technical Information of China (English)

    Zhenbao Yu; Gillian Vogel; Yan Coulombe; Danielle Dubeau; Elizabeth Spehalski; Josée Hébert; David O Ferguson; Jean Yves Masson; Stéphane Richard

    2012-01-01

    The MRE11/RAD50/NBS1 complex is the primary sensor rapidly recruited to DNA double-strand breaks (DSBs).MRE11 is known to be arginine methylated by PRMT1 within its glycine-arginine-rich (GAR) motif.In this study,we report a mouse knock-in allele of Mre11 that substitutes the arginines with lysines in the GAR motif and generates the MRE11RK protein devoid of methylated arginines.The Mre11RK/RK mice were hypersensitive to γ-irradiation (IR) and the cells from these mice displayed cell cycle checkpoint defects and chromosome instability.Moreover,the Mre11RK/RK MEFs exhibited ATR/CHK1 signaling defects and impairment in the recruitment of RPA and RAD51 to the damaged sites.The MRKRN complex formed and localized to the sites of DNA damage and normally activated the ATM pathway in response to IR.The MRKRN complex exhibited exonuclease and DNA-binding defects in vitro responsible for the impaired DNA end resection and ATR activation observed in vivo in response to IR.Our findings provide genetic evidence for the critical role of the MRE11 GAR motif in DSB repair,and demonstrate a mechanistic link between post-translational modifications at the MRE11 GAR motif and DSB processing,as well as the ATR/CHK1 checkpoint signaling.

  1. A Genome-wide CRISPR Screen Identifies CDC25A as a Determinant of Sensitivity to ATR Inhibitors.

    Science.gov (United States)

    Ruiz, Sergio; Mayor-Ruiz, Cristina; Lafarga, Vanesa; Murga, Matilde; Vega-Sendino, Maria; Ortega, Sagrario; Fernandez-Capetillo, Oscar

    2016-04-21

    One recurring theme in drug development is to exploit synthetic lethal properties as means to preferentially damage the DNA of cancer cells. We and others have previously developed inhibitors of the ATR kinase, shown to be particularly genotoxic for cells expressing certain oncogenes. In contrast, the mechanisms of resistance to ATR inhibitors remain unexplored. We report here on a genome-wide CRISPR-Cas9 screen that identified CDC25A as a major determinant of sensitivity to ATR inhibition. CDC25A-deficient cells resist high doses of ATR inhibitors, which we show is due to their failure to prematurely enter mitosis in response to the drugs. Forcing mitotic entry with WEE1 inhibitors restores the toxicity of ATR inhibitors in CDC25A-deficient cells. With ATR inhibitors now entering the clinic, our work provides a better understanding of the mechanisms by which these compounds kill cells and reveals genetic interactions that could be used for their rational use. PMID:27067599

  2. Cleaning-in-place of immunoaffinity resins monitored by in situ ATR-FTIR spectroscopy.

    Science.gov (United States)

    Boulet-Audet, Maxime; Byrne, Bernadette; Kazarian, Sergei G

    2015-09-01

    In the next 10 years, the pharmaceutical industry anticipates that revenue from biotherapeutics will overtake those generated from small drug molecules. Despite effectively treating a range of chronic and life-threatening diseases, the high cost of biotherapeutics limits their use. For biotherapeutic monoclonal antibodies (mAbs), an important production cost is the affinity resin used for protein capture. Cleaning-in-place (CIP) protocols aim to optimise the lifespan of the resin by slowing binding capacity decay. Binding assays can determine resin capacity from the mobile phase, but do not reveal the underlying causes of Protein A ligand degradation. The focus needs to be on the stationary phase to examine the effect of CIP on the resin. To directly determine both the local Protein A ligand concentration and conformation on two Protein A resins, we developed a method based on attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy. ATR-FTIR spectroscopic imaging revealed that applying a carefully controlled load to agarose beads produces an even and reproducible contact with the internal reflection element. This allowed detection and quantification of the binding capacity of the stationary phase. ATR-FTIR spectroscopy also showed that Protein A proteolysis does not seem to occur under typical CIP conditions (below 1 M NaOH). However, our data revealed that concentrations of NaOH above 0.1 M cause significant changes in Protein A conformation. The addition of >0.4 M trehalose during CIP significantly reduced NaOH-induced ligand unfolding observed for one of the two Protein A resins tested. Such insights could help to optimise CIP protocols in order to extend resin lifetime and reduce mAb production costs. PMID:26159572

  3. Radiosensitization of Human Leukemic HL-60 Cells by ATR Kinase Inhibitor (VE-821: Phosphoproteomic Analysis

    Directory of Open Access Journals (Sweden)

    Barbora Šalovská

    2014-07-01

    Full Text Available DNA damaging agents such as ionizing radiation or chemotherapy are frequently used in oncology. DNA damage response (DDR—triggered by radiation-induced double strand breaks—is orchestrated mainly by three Phosphatidylinositol 3-kinase-related kinases (PIKKs: Ataxia teleangiectasia mutated (ATM, DNA-dependent protein kinase (DNA-PK and ATM and Rad3-related kinase (ATR. Their activation promotes cell-cycle arrest and facilitates DNA damage repair, resulting in radioresistance. Recently developed specific ATR inhibitor, VE-821 (3-amino-6-(4-(methylsulfonylphenyl-N-phenylpyrazine-2-carboxamide, has been reported to have a significant radio- and chemo-sensitizing effect delimited to cancer cells (largely p53-deficient without affecting normal cells. In this study, we employed SILAC-based quantitative phosphoproteomics to describe the mechanism of the radiosensitizing effect of VE-821 in human promyelocytic leukemic cells HL-60 (p53-negative. Hydrophilic interaction liquid chromatography (HILIC-prefractionation with TiO2-enrichment and nano-liquid chromatography—tandem mass spectrometry (LC-MS/MS analysis revealed 9834 phosphorylation sites. Proteins with differentially up-/down-regulated phosphorylation were mostly localized in the nucleus and were involved in cellular processes such as DDR, all phases of the cell cycle, and cell division. Moreover, sequence motif analysis revealed significant changes in the activities of kinases involved in these processes. Taken together, our data indicates that ATR kinase has multiple roles in response to DNA damage throughout the cell cycle and that its inhibitor VE-821 is a potent radiosensitizing agent for p53-negative HL-60 cells.

  4. Root discrimination of closely related crop and weed species using FT MIR-ATR spectroscopy.

    Science.gov (United States)

    Meinen, Catharina; Rauber, Rolf

    2015-01-01

    Root discrimination of species is a pre-condition for studying belowground competition processes between crop and weed species. In this experiment, we tested Fourier transform mid-infrared (FT MIR)-attenuated total reflection (ATR) spectroscopy to discriminate roots of closely related crop and weed species grown in the greenhouse: maize/barnyard grass, barley/wild oat, wheat/blackgrass (Poaceae), and sugar beet/common lambsquarters (Chenopodiaceae). Fresh (moist) and dried root segments as well as ground roots were analyzed by FT MIR-ATR spectroscopy. Root absorption spectra showed species specific peak distribution and peak height. A clear separation according to species was not possible with fresh root segments. Dried root segments (including root basis, middle section, and root tip) of maize/barnyard grass and sugar beet/common lambsquarters formed completely separated species clusters. Wheat and blackgrass separated in species specific clusters when root tips were removed from cluster analysis. A clear separation of dried root segments according to species was not possible in the case of barley and wild oat. Cluster analyses of ground roots revealed a 100% separation of all tested crop and weed species combinations. Spectra grouped in Poaceae and Chenopodiaceae clusters. Within the Poaceae cluster, C3 and C4 species differed significantly in heterogeneity. Thus, root spectra reflected the degree of kinship. To quantify species proportion in root mixtures, a two- and a three-species model for species quantification in root mixtures of maize, barnyard grass, and wild oat was calculated. The models showed low standard errors of prediction (RMSEP) and high residual predictive deviation values in an external test set validation. Hence, FT MIR-ATR spectroscopy seems to be a promising tool for root research even between closely related plant species. PMID:26483799

  5. Root discrimination of closely related crop and weed species using FT MIR-ATR spectroscopy

    Directory of Open Access Journals (Sweden)

    Catharina eMeinen

    2015-09-01

    Full Text Available Root discrimination of species is a pre-condition for studying belowground competition processes between crop and weed species. In this experiment, we tested Fourier transform mid-infrared (FT MIR-attenuated total reflection (ATR spectroscopy to discriminate roots of closely related crop and weed species grown in the greenhouse: maize/barnyard grass, barley/wild oat, wheat/blackgrass (Poaceae, and sugar beet/common lambsquarters (Chenopodiaceae. Fresh (moist and dried root segments as well as ground roots were analyzed by FT MIR-ATR spectroscopy. Root absorption spectra showed species specific peak distribution and peak height. A clear separation according to species was not possible with fresh root segments. Dried root segments (including root basis, middle section and root tip of maize/barnyard grass and sugar beet/common lambsquarters formed completely separated species clusters. Wheat and blackgrass separated in species specific clusters when root tips were removed from cluster analysis. A clear separation of dried root segments according to species was not possible in the case of barley and wild oat. Cluster analyses of ground roots revealed a 100 % separation of all tested crop and weed species combinations. Spectra grouped in Poaceae and Chenopodiaceae clusters. Within the Poaceae cluster, C3 and C4 species differed significantly in heterogeneity. Thus, root spectra reflected the degree of kinship. To quantify species proportion in root mixtures, a two- and a three-species model for species quantification in root mixtures of maize, barnyard grass, and wild oat was calculated. The models showed low standard errors of prediction (RMSEP and high residual predictive deviation (RPD values in an external test set validation. Hence, FT MIR-ATR spectroscopy seems to be a promising tool for root research even between closely related plant species.

  6. STAT3 interrupts ATR-Chk1 signaling to allow oncovirus-mediated cell proliferation.

    Science.gov (United States)

    Koganti, Siva; Hui-Yuen, Joyce; McAllister, Shane; Gardner, Benjamin; Grasser, Friedrich; Palendira, Umaimainthan; Tangye, Stuart G; Freeman, Alexandra F; Bhaduri-McIntosh, Sumita

    2014-04-01

    DNA damage response (DDR) is a signaling network that senses DNA damage and activates response pathways to coordinate cell-cycle progression and DNA repair. Thus, DDR is critical for maintenance of genome stability, and presents a powerful defense against tumorigenesis. Therefore, to drive cell-proliferation and transformation, viral and cellular oncogenes need to circumvent DDR-induced cell-cycle checkpoints. Unlike in hereditary cancers, mechanisms that attenuate DDR and disrupt cell-cycle checkpoints in sporadic cancers are not well understood. Using Epstein-Barr virus (EBV) as a source of oncogenes, we have previously shown that EBV-driven cell proliferation requires the cellular transcription factor STAT3. EBV infection is rapidly followed by activation and increased expression of STAT3, which mediates relaxation of the intra-S phase cell-cycle checkpoint; this facilitates viral oncogene-driven cell proliferation. We now show that replication stress-associated DNA damage, which results from EBV infection, is detected by DDR. However, signaling downstream of ATR is impaired by STAT3, leading to relaxation of the intra-S phase checkpoint. We find that STAT3 interrupts ATR-to-Chk1 signaling by promoting loss of Claspin, a protein that assists ATR to phosphorylate Chk1. This loss of Claspin which ultimately facilitates cell proliferation is mediated by caspase 7, a protein that typically promotes cell death. Our findings demonstrate how STAT3, which is constitutively active in many human cancers, suppresses DDR, fundamental to tumorigenesis. This newly recognized role for STAT3 in attenuation of DDR, discovered in the context of EBV infection, is of broad interest as the biology of cell proliferation is central to both health and disease.

  7. Hydraulic Shuttle Irradiation System (HSIS) Recently Installed in the Advanced Test Reactor (ATR)

    International Nuclear Information System (INIS)

    Most test reactors are equipped with shuttle facilities (sometimes called rabbit tubes) whereby small capsules can be inserted into the reactor and retrieved during power operations. With the installation of Hydraulic Shuttle Irradiation System (HSIS) this capability has been restored to the Advanced Test Reactor (ATR) at Idaho National Laboratory (INL). The general design and operating principles of this system were patterned after the hydraulic rabbit at Oak Ridge National Laboratory's (ORNL) High Flux Isotope Reactor (HFIR), which has operated successfully for many years. Using primary coolant as the motive medium the HSIS system is designed to simultaneously transport fourteen shuttle capsules, each 16 mm OD x 57 mm long, to and from the B-7 position of the reactor. The B-7 position is one of the higher flux positions in the reactor with typical thermal and fast (>1 Mev) fluxes of 2.8E+14 n/cm2/sec and 1.9E+14 n/cm2/sec respectively. The available space inside each shuttle is approximately 14 mm diameter x 50 mm long. The shuttle containers are made from titanium which was selected for its low neutron activation properties and durability. Shuttles can be irradiated for time periods ranging from a few minutes to several months. The Send and Receive Station (SRS) for the HSIS is located 2.5 m deep in the ATR canal which allows irradiated shuttles to be easily moved from the SRS to a wet loaded cask, or transport pig. The HSIS system first irradiated (empty) shuttles in September 2009 and has since completed a Readiness Assessment in November 2009. The HSIS is a key component of the ATR National Scientific User Facility (NSUF) operated by Battelle Energy Alliance, LLC and is available to a wide variety of university researchers for nuclear fuels and materials experiments as well as medical isotope research and production.

  8. Tn5-mutagenesis and identification of atr operon and trpE gene responsible for indole-3-acetic acid synthesis in Azospirillum brasilense Yu62

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    To bring more information about synthesis of indole-3-acetic acid (IAA) from Azospirillum brasilense, a Tn5-insertion library of A. brasilense Yu62 was constructed and subjected to screening for IAA producing mutants. Two mutants with decreased IAA levels, named as A3 and A24, were isolated. The sequence analysis of loci tagged showed that the Tn5-1063a was located in the atrA gene encoding GntR family transcriptional regulator and trpE gene encoding component I of anthranilate synthase respectively. At the same time, atrB encoding phosphotransferase and atrC encoding aminotransferase were cloned downstream the atrA gene and atrA,atrB and atrC were clustered in an operon. Mutagenesis and complementation studies showed that atrA and atrC were involved in IAA synthesis. IAA levels of trpE mutant and wild-type strain could be improved by adding anthranilate into the medium.

  9. AGR-3/4 Final Data Qualification Report for ATR Cycles 151A through 155B-1

    Energy Technology Data Exchange (ETDEWEB)

    Pham, Binh T. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-03-01

    This report provides the qualification status of experimental data for the entire Advanced Gas Reactor 3/4 (AGR 3/4) fuel irradiation. AGR-3/4 is the third in a series of planned irradiation experiments conducted in the Advanced Test Reactor (ATR) at Idaho National Laboratory (INL) for the AGR Fuel Development and Qualification Program, which supports development of the advanced reactor technology under the INL ART Technology Development Office (TDO). The main objective of AGR-3/4 irradiation is to provide a known source of fission products for subsequent transport through compact matrix and structural graphite materials due to the presence of designed-to-fail fuel particles. Full power irradiation of the AGR 3/4 test began on December 14, 2011 (ATR Cycle 151A), and was completed on April 12, 2014 (end of ATR Cycle 155B) after 369.1 effective full power days of irradiation. The AGR-3/4 test was in the reactor core for eight of the ten ATR cycles between 151A and 155B. During the unplanned outage cycle, 153A, the experiment was removed from the ATR northeast flux trap (NEFT) location and stored in the ATR canal. This was to prevent overheating of fuel compacts due to higher than normal ATR power during the subsequent Powered Axial Locator Mechanism cycle, 153B. The AGR 3/4 test was inserted back into the ATR NEFT location during the outage of ATR Cycle 154A on April 26, 2013. Therefore, the AGR-3/4 irradiation data received during these 2 cycles (153A and 153B) are irrelevant and their qualification status isnot included in this report. Additionally, during ATR Cycle 152A the ATR core ran at low power for a short enough duration that the irradiation data are not used for physics and thermal calculations. However, the qualification status of irradiation data for this cycle is still covered in this report. As a result, this report includes data from 8 ATR Cycles: 151A, 151B, 152A, 152B, 154A, 154B, 155A, and 155B, as recorded in the Nuclear Data Management and

  10. FTIR-ATR monitoring and SEC/RI/MALLS characterization of ATRP synthesized hyperbranched polyacrylates

    OpenAIRE

    Gonçalves, Miguel; Pinto, Virgínia; Dias, Rolando; Costa, Mário Rui

    2010-01-01

    This work reports the synthesis at 1 L scale of hyperbranched polyacrylates based upon acrylate/diacrylate monomers such as n-butyl acrylate (nBA)/1,6-hexanediol diacrylate (HDDA) and using atom transfer radical polymerization (ATRP). A FTIR-ATR immersion probe was used to monitor the polymerization reaction. The dynamics of the build-up of polymer structure was studied by off-line analysis of samples at different reaction times by size exclusion chromatography (SEC) with detection of refract...

  11. Hydraulic Shuttle Irradiation System (HSIS) Recently Installed in the Advanced Test Reactor (ATR)

    Energy Technology Data Exchange (ETDEWEB)

    A. Joseph Palmer; Gerry L. McCormick; Shannon J. Corrigan

    2010-06-01

    2010 International Congress on Advances in Nuclear Power Plants (ICAPP’10) ANS Annual Meeting Imbedded Topical San Diego, CA June 13 – 17, 2010 Hydraulic Shuttle Irradiation System (HSIS) Recently Installed in the Advanced Test Reactor (ATR) Author: A. Joseph Palmer, Mechanical Engineer, Irradiation Test Programs, 208-526-8700, Joe.Palmer@INL.gov Affiliation: Idaho National Laboratory P.O. Box 1625, MS-3840 Idaho Falls, ID 83415 INL/CON-10-17680 ABSTRACT Most test reactors are equipped with shuttle facilities (sometimes called rabbit tubes) whereby small capsules can be inserted into the reactor and retrieved during power operations. With the installation of Hydraulic Shuttle Irradiation System (HSIS) this capability has been restored to the Advanced Test Reactor (ATR) at Idaho National Laboratory (INL). The general design and operating principles of this system were patterned after the hydraulic rabbit at Oak Ridge National Laboratory’s (ORNL) High Flux Isotope Reactor (HFIR), which has operated successfully for many years. Using primary coolant as the motive medium the HSIS system is designed to simultaneously transport fourteen shuttle capsules, each 16 mm OD x 57 mm long, to and from the B-7 position of the reactor. The B-7 position is one of the higher flux positions in the reactor with typical thermal and fast (>1 Mev) fluxes of 2.8E+14 n/cm2/sec and 1.9E+14 n/cm2/sec respectively. The available space inside each shuttle is approximately 14 mm diameter x 50 mm long. The shuttle containers are made from titanium which was selected for its low neutron activation properties and durability. Shuttles can be irradiated for time periods ranging from a few minutes to several months. The Send and Receive Station (SRS) for the HSIS is located 2.5 m deep in the ATR canal which allows irradiated shuttles to be easily moved from the SRS to a wet loaded cask, or transport pig. The HSIS system first irradiated (empty) shuttles in September 2009 and has since completed

  12. Registration of heavy metal ions and pesticides with ATR planar waveguide enzyme sensors

    Science.gov (United States)

    Nabok, Alexei; Haron, Saharudin; Ray, Asim

    2004-11-01

    The proposed novel type of enzyme optical sensors is based on a combination of SiO2/Si3N4/SiO2 planar waveguide ATR (attenuated total reflection) transducer, fabricated by standard silicon planar technology, with the composite polyelectrolyte self-assembled coating containing both organic chromophores and enzyme molecules. Such devices were deployed to monitor typical industrial and agricultural water pollutants, such as heavy metal ions and pesticides, acting as inhibitors of enzyme reactions. The sensitivity of registration of these pollutants in the range of 1 ppb was achieved. The use of different enzymes in the sensitive membrane provides a background for pattern recognition of the above pollutants.

  13. Characterisation Of Polysacharides And Lipids From Selected Green Algae Species By FTIR-ATR Spectroscopy

    Directory of Open Access Journals (Sweden)

    Bartošová Alica

    2015-06-01

    Full Text Available Fourier transform infrared (FTIR spectroscopy was used in this study to identify and determine spectral features of Chromochloris zofingiensis (Dönz Fucíková et L.A. Lewis (SAG 211-14, Gottingen, Germany, Acutodesmus obliguus (Turpin Hegewald (SAG 276-1, Gottingen, Germany and Chlorella sorokiniana (K. Brandt Pröschold et Darienko (SAG 211-40c, Gottingen, Germany. Polysaccharides and lipids from these three algae species were determined using Fourier Transformed Infrared Spectroscopy (FTIR with ATR accessory with diamante crystal in spectral range from 400 – 4000 cm−1 and resolution 4.

  14. An add-on system for photochemical ATR-IR spectroscopy studies

    DEFF Research Database (Denmark)

    2014-01-01

    The invention relates to an add-on system for a unit mainly adapted for attenuated total reflectance infrared (ATR-IR) spectroscopy. The add-on system enables time-resolved in-situ measurements of different sample types in an easy, simple and inexpensive way. The add-on system includes a cap (300G......) and the cap (300B,300H,300J), a source of light, e.g. a light emitting diode (418) or an optical fibre (416) connectable to a laser, and a light source containing cavity....

  15. Radar automatic target recognition (ATR) and non-cooperative target recognition (NCTR)

    CERN Document Server

    Blacknell, David

    2013-01-01

    The ability to detect and locate targets by day or night, over wide areas, regardless of weather conditions has long made radar a key sensor in many military and civil applications. However, the ability to automatically and reliably distinguish different targets represents a difficult challenge. Radar Automatic Target Recognition (ATR) and Non-Cooperative Target Recognition (NCTR) captures material presented in the NATO SET-172 lecture series to provide an overview of the state-of-the-art and continuing challenges of radar target recognition. Topics covered include the problem as applied to th

  16. Investigation on the adsorption characteristics of sodium benzoate and taurine on gold nanoparticle film by ATR-FTIR spectroscopy

    Science.gov (United States)

    Kumar, Naveen; Thomas, S.; Tokas, R. B.; Kshirsagar, R. J.

    2014-01-01

    Fourier transform infrared (FTIR) spectroscopic studies of sodium benzoate and taurine adsorbed on gold nanoparticle (AuNp) film on silanised glass slides have been studied by attenuated total reflection technique (ATR). The surface morphology of the AuNp films has been measured by Atomic Force Microscopy. The ATR spectra of sodium benzoate and taurine deposited on AuNp film are compared with ATR spectra of their powdered bulk samples. A new red-shifted band appeared along with the symmetric and asymmetric stretches of carboxylate group of sodium benzoate leading to a broadening of the above peaks. Similar behavior is also seen in the case of symmetric and asymmetric stretches of sulphonate group of taurine. The results indicate presence of both chemisorbed and physisorbed layers of both sodium benzoate and taurine on the AuNp film with bottom layer chemically bound to AuNp through carboxylate and sulphonate groups respectively.

  17. Molecular orientation of molybdate ions adsorbed on goethite nanoparticles revealed by polarized in situ ATR-IR spectroscopy

    Science.gov (United States)

    Davantès, Athénaïs; Lefèvre, Grégory

    2016-11-01

    The speciation of species adsorbed on nanoparticles is a major concern for several fields, as environmental pollution and remediation, surface functionalization, or catalysis. Attenuated total reflectance infrared spectroscopy (ATR-IR) was amongst the rare methods able to give in situ information about the geometry of surface complexes on nanoparticles. A new possibility using this technique is illustrated here with the MoO42 -/goethite system. Using deuterated goethite to avoid spectral interferences, adsorption of molybdate ions on a spontaneous oriented film of nanoparticles has been followed using a polarized infrared beam. From the decomposition of spectra in the x, y and z directions, a monodentate surface complex on the {101} faces has been found as the most probable geometry. This result demonstrates that polarized ATR-IR allows to characterize in more details adsorption mode at the atomic scale, in comparison with usual ATR-IR spectroscopy.

  18. In-column ATR-FTIR spectroscopy to monitor affinity chromatography purification of monoclonal antibodies

    Science.gov (United States)

    Boulet-Audet, Maxime; Kazarian, Sergei G.; Byrne, Bernadette

    2016-07-01

    In recent years many monoclonal antibodies (mAb) have entered the biotherapeutics market, offering new treatments for chronic and life-threatening diseases. Protein A resin captures monoclonal antibody (mAb) effectively, but the binding capacity decays over repeated purification cycles. On an industrial scale, replacing fouled Protein A affinity chromatography resin accounts for a large proportion of the raw material cost. Cleaning-in-place (CIP) procedures were developed to extend Protein A resin lifespan, but chromatograms cannot reliably quantify any remaining contaminants over repeated cycles. To study resin fouling in situ, we coupled affinity chromatography and Fourier transform infrared (FTIR) spectroscopy for the first time, by embedding an attenuated total reflection (ATR) sensor inside a micro-scale column while measuring the UV 280 nm and conductivity. Our approach quantified the in-column protein concentration in the resin bed and determined protein conformation. Our results show that Protein A ligand leached during CIP. We also found that host cell proteins bound to the Protein A resin even more strongly than mAbs and that typical CIP conditions do not remove all fouling contaminants. The insights derived from in-column ATR-FTIR spectroscopic monitoring could contribute to mAb purification quality assurance as well as guide the development of more effective CIP conditions to optimise resin lifespan.

  19. The nucleolus stress response is coupled to an ATR-Chk1-mediated G2 arrest.

    Science.gov (United States)

    Ma, Hanhui; Pederson, Thoru

    2013-05-01

    We report experiments on the connection between nucleolar stress and cell cycle progression, using HeLa cells engineered with the fluorescent ubiquitinylation-based cell cycle indicator. Nucleolar stress elicited by brief exposure of cells to a low concentration of actinomycin D that selectively inhibits rRNA synthesis had no effect on traverse of G1 or S, but stalled cells in very late interphase. Additional experiments revealed that a switch occurs during a specific temporal window during nucleolar stress and that the subsequent cell cycle arrest is not triggered simply by the stress-induced decline in the synthesis of rRNA or by a ribosome starvation phenomenon. Further experiments revealed that this nucleolus stress-induced cell cycle arrest involves the action of a G2 checkpoint mediated by the ataxia telangiectasia and Rad3-related protein (ATR)-checkpoint kinase 1 (Chk1) pathway. Based on analysis of the cell cycle stages at which this nucleolar stress effect is put into action, to become manifest later, our results demonstrate a feedforward mechanism that leads to G2 arrest and identify ATR and Chk1 as molecular agents of the requisite checkpoint.

  20. ATR-FTIR measurements of albumin and fibrinogen adsorption: Inert versus calcium phosphate ceramics.

    Science.gov (United States)

    Boix, Marcel; Eslava, Salvador; Costa Machado, Gil; Gosselin, Emmanuel; Ni, Na; Saiz, Eduardo; De Coninck, Joël

    2015-11-01

    Arthritis, bone fracture, bone tumors and other musculoskeletal diseases affect millions of people across the world. Nowadays, inert and bioactive ceramics are used as bone substitutes or for bone regeneration. Their bioactivity is very much dictated by the way proteins adsorb on their surface. In this work, we compared the adsorption of albumin and fibrinogen on inert and calcium phosphates ceramics (CaPs) using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) to follow in situ protein adsorption on these materials. To this effect, we developed a sol-gel technique to control the surface chemistry of an ATR-FTIR detector. Hydroxyapatite adsorbed more albumin and β-tricalcium phosphate adsorbed more fibrinogen. Biphasic calcium phosphate presented the lowest adsorption among CaP for both proteins, illustrating the effect of surface heterogeneities. Inert ceramics adsorbed a lower amount of both proteins compared with bioactive ceramics. A significant change was observed in the conformation of the adsorbed protein versus the surface chemistry. Hydroxyapatite produced a larger loss of α-helix structure on albumin and biphasic calcium phosphate reduced β-sheet percentage on fibrinogen. Inert ceramics produced large α-helix loss on albumin and presented weak interaction with fibrinogen. Zirconia did not adsorb albumin and titanium dioxide promoted huge denaturalization of fibrinogen.

  1. Analytical characterization of polymers used in conservation and restoration by ATR-FTIR spectroscopy.

    Science.gov (United States)

    Chércoles Asensio, Ruth; San Andrés Moya, Margarita; de la Roja, José Manuel; Gómez, Marisa

    2009-12-01

    In the last few decades many new polymers have been synthesized that are now being used in cultural heritage conservation. The physical and chemical properties and the long-term behaviors of these new polymers are determined by the chemical composition of the starting materials used in their synthesis along with the nature of the substances added to facilitate their production. The practical applications of these polymers depend on their composition and form (foam, film, sheets, pressure-sensitive adhesives, heat-seal adhesives, etc.). Some materials are used in restoration works and others for the exhibition, storage and transport of works of art. In all cases, it is absolutely necessary to know their compositions. Furthermore, many different materials that are manufactured for other objectives are also used for conservation and restoration. The technical information about the materials provided by the manufacturer is usually incomplete, so it is necessary to analytically characterize such materials. FTIR spectrometry is widely used for polymer identification, and, more recently, ATR-FTIR has been shown to give excellent results. This paper reports the ATR-FTIR analysis of samples of polymeric materials used in the conservation of artworks. These samples were examined directly in the solid material without sample preparation.

  2. Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic analysis of regenerated bone

    Science.gov (United States)

    Benetti, Carolina; Kazarain, Sergei G.; Alves, Marco A. V.; Blay, Alberto; Correa, Luciana; Zezell, Denise M.

    2014-03-01

    The cutting of bone is routinely required in medical procedures, especially in dental applications. In such cases, bone regeneration and new bone quality can determine the success of the treatment. This study investigated the main spectral differences of undamaged and healed bone using the ATR-FTIR spectroscopy technique. Three rabbits were submitted to a surgical procedure; a small piece of bone (3x3 mm2) was removed from both sides of their jaws using a high speed drill. After 15 days, the rabbits were euthanized and the jaws were removed. A bone slice was cut from each side of the jaw containing regions of undamaged and newly formed bone, resulting in six samples which were polished for spectroscopic comparison. The samples were analyzed by FTIR spectroscopy using a diamond ATR accessory. Spectral characteristics were compared and particular attention was paid to the proportion of phosphate to amide I bands and the width of the phosphate band. The results show that the ratio of phosphate to amide I is smaller in new bone tissue than in the undamaged bone, indicating a higher organic content in the newly formed bone. The analysis of the width of the phosphate band suggests a crystallinity difference between both tissues, since the width was higher in the new bone than in the natural bone. These results suggest that the differences observed in bone aging processes by FTIR spectroscopic can be applied to the study of healing processes.

  3. Diesel fuel processor for PEM fuel cells: Two possible alternatives (ATR versus SR)

    Science.gov (United States)

    Cutillo, A.; Specchia, S.; Antonini, M.; Saracco, G.; Specchia, V.

    There are large efforts in exploring the on-board reforming technologies, which would avoid the actual lack of hydrogen infrastructure and related safety issues. From this view point, the present work deals with the comparison between two different 10 kW e fuel processors (FP) systems for the production of hydrogen-rich fuel gas starting from diesel oil, based respectively on autothermal (ATR) and steam-reforming (SR) process and related CO clean-up technologies; the obtained hydrogen rich gas is fed to the PEMFC stack of an auxiliary power unit (APU). Based on a series of simulations with Matlab/Simulink, the two systems were compared in terms of FP and APU efficiency, hydrogen concentration fed to the FC, water balance and process scheme complexity. Notwithstanding a slightly higher process scheme complexity and a slightly more difficult water recovery, the FP based on the SR scheme, as compared to the ATR one, shows higher efficiency and larger hydrogen concentration for the stream fed to the PEMFC anode, which represent key issues for auxiliary power generation based on FCs as compared, e.g. to alternators.

  4. DBC1 phosphorylation by ATM/ATR inhibits SIRT1 deacetylase in response to DNA damage

    Institute of Scientific and Technical Information of China (English)

    Laura Zannini; Giacomo Buscemi; Ja-Eun Kim; Enrico Fontanella; Domenico Delia

    2012-01-01

    Human DBC1 (deleted in breast cancer-1; KIAA1967) is a nuclear protein that,in response to DNA damage,competitively inhibits the NAD+-dependent deacetylase SIRT1,a regulator of p53 apoptotic functions in response to genotoxic stress.DBC1 depletion in human cells increases SIRT1 activity,resulting in the deacetylation of p53 and protection from apoptosis.However,the mechanisms regulating this process have not yet been determined.Here,we report that,in human cell lines,DNA damage triggered the phosphorylation of DBC1 on Thr454 by ATM (ataxia telangiectasia-mutated) and ATR (ataxia telangiectasia and Rad3-related)kinases.Phosphorylated DBC1 bound to and inhibited SIRT1,resulting in the dissociation of the SIRT1-p53 complex and stimulating p53 acetylation and p53-dependent cell death.Indeed,DBC1-mediated genotoxicity,which was shown in knockdown experiments to be dependent on SIRT1 and p53 expression,was defective in cells expressing the phospho-mutant DBC1T454A.This study describes the first post-translational modification of DBC1 and provides new mechanistic insight linking ATM/ATR to the DBC1-SIRT1-p53 apoptotic axis triggered by DNA damage.

  5. Independent Review of AFC 2A, 2B, and 2E ATR Irradiation Tests

    Energy Technology Data Exchange (ETDEWEB)

    M. Cappiello; R. Hobbins; K. Penny; L. Walters

    2014-01-01

    As part of the Department of Energy Advanced Fuel Cycle program, a series of fuels development irradiation tests have been performed in the Advanced Test Reactor (ATR) at the Idaho National Laboratory. These tests are providing excellent data for advanced fuels development. The program is focused on the transmutation of higher actinides which best can be accomplished in a sodium-cooled fast reactor. Because a fast test reactor is no longer available in the US, a special test vehicle is used to achieve near-prototypic fast reactor conditions (neutron spectra and temperature) for use in ATR (a water-cooled thermal reactor). As part of the testing program, there were many successful tests of advanced fuels including metals and ceramics. Recently however, there have been three experimental campaigns using metal fuels that experienced failure during irradiation. At the request of the program, an independent review committee was convened to review the post-test analyses performed by the fuels development team, to assess the conclusions of the team for the cause of the failures, to assess the adequacy and completeness of the analyses, to identify issues that were missed, and to make recommendations for improvements in the design and operation of future tests. Although there is some difference of opinion, the review committee largely agreed with the conclusions of the fuel development team regarding the cause of the failures. For the most part, the analyses that support the conclusions are sufficient.

  6. Characterization of Printing Inks Using DART-Q-TOF-MS and Attenuated Total Reflectance (ATR) FTIR.

    Science.gov (United States)

    Williamson, Rhett; Raeva, Anna; Almirall, Jose R

    2016-05-01

    The rise in improved and widely accessible printing technology has resulted in an interest to develop rapid and minimally destructive chemical analytical techniques that can characterize printing inks for forensic document analysis. Chemical characterization of printing inks allows for both discrimination of inks originating from different sources and the association of inks originating from the same source. Direct analysis in real-time mass spectrometry (DART-MS) and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) were used in tandem to analyze four different classes of printing inks: inkjets, toners, offset, and intaglio. A total of 319 samples or ~ 80 samples from each class were analyzed directly on a paper substrate using the two methods. DART-MS was found to characterize the semi-volatile polymeric vehicle components, while ATR-FTIR provided chemical information associated with the bulk components of these inks. Complimentary data results in improved discrimination when both techniques are used in succession resulting in >96% discrimination for all toners, 95% for all inkjets, >92% for all offset, and >54% for all intaglio inks. PMID:27122410

  7. APLIKASI SISTEM INFORMASI PERTANAHAN BERDASARKAN JENIS-JENIS HAK ATAS TANAH DI KANTOR PERTANAHAN KOTA SURABAYA II (STUDI KASUS KELURAHAN GENTENG, KECAMATAN GENTENG, SURABAYA

    Directory of Open Access Journals (Sweden)

    Ery Abdul Baary

    2015-02-01

    Full Text Available Di Kantor Pertanahan Kota Surabaya II, sistem informasi pertanahan belum diterapkan, jadi untuk melaksanakan tugasnya hanya menggunakan sebatas peta digital, maka dari itu perlu adanya sistem informasi pertanahan yang terpadu dan mutakhir untuk mendukung dan mengoptimalkan pengelolaan sistem informasi pertanahan di Kota Surabaya II.Pada penelitian ini akan dilakukan pembuatan sistem informasi pertanahan berdasarkan jenis hak atas tanah dengan menggunakan peta digital Kelurahan Genteng, Kecamatan Genteng, Surabaya skala 1:1000, citra Quickbird 2010, database mengenai bidang tanah dan data hasil survey. Pengolahan data menggunakan software utama Autodesk Land Dekstop 2009, ArcGIS 9.3. Pembuatan program aplikasi menggunakan software Visual Basic 6.0 yang dilengkapi dengan software tambahan MapObject 2.2.Dari hasil pembuatan Sistem informasi pertanahan di Kelurahan Genteng, Program ini mampu mengidentifikasi informasi bidang tanah mengenai jenis hak atas tanah yang meliputi 224 bidang tanah yang terdiri dari 132 bidang tanah atas hak guna bangunan, 89 bidang tanah atas hak milik, dan 3 bidang tanah atas hak pakai, serta dilengkapi dengan buku panduan sehingga dapat digunakan untuk membantu dalam pengambilan kebijakan dalam rencana pelaksanaan program inventarisasi penguasaan, pemilikan, penggunaan, dan pemanfaatan tanah.

  8. Pembuatan Standard Operating Procedure (SOP Service Desk Berdasarkan Kerangka Kerja Itil V3 dengan Menggunakan Metode Analisis Gap Layanan (Studi Kasus: PT. XYZ , Tangerang

    Directory of Open Access Journals (Sweden)

    Annisa Rachmi

    2014-09-01

    Full Text Available Penggunaan Teknologi Informasi di banyak perusahaan telah menjadi satu hal penting dalam meningkatkan efektifitas dan efisiensi operasional bisnis yang mendukung tercapainya tujuan perusahaan, termasuk juga PT XYZ. Unit fungsional service desk sangat dibutuhkan oleh PT XYZ dalam mendukung operasional TI dan menangani permasalahan yang muncul serta memonitor terkait penggunaan TI yang ada di dalam perusahaan. Adanya kebutuhan PT XYZ untuk menjadikan service desk menjadi unit yang dapat berjalan dengan baik sesuai dengan fungsinya, menunjukkan bahwa perlunya penerapan tata kelola TI untuk mengatur dan mengelola aktivitas service desk. Salah satu tata kelola TI yang dimaksud adalah berupa prosedur kerja. Kerangka kerja Information Technology Infrastructure Library (ITIL merupakan suatu best practice yang bertujuan secara berkelanjutan meningkatkan efisiensi operasional TI. Perancangan dan implementasi service desk berdasarkan kerangka kerja ITIL V3 di PT XYZ diperlukan dalam upaya meningkatkan layanan TI dan mengatasi masalah yang ada saat ini. Pembuatan SOP ini diinisialisasi dengan menggunakan metode analisis gap, proses-proses yang dimuat ke dalam SOP merupakan hasil analisis kesenjangan antara kondisi kekinian service desk PT XYZ dengan kondisi ideal service desk framework ITIL.

  9. EVALUASI KEAMANAN INFORMASI PADA PTI PDAM TIRTA MOEDAL KOTA SEMARANG BERDASARKAN INDEKS KEAMANAN INFORMASI SNI ISO/IEC 27001:2009

    Directory of Open Access Journals (Sweden)

    Diah Restu Wardani

    2015-10-01

    Full Text Available Adanya rencana untuk meningkatkan keamanan informasi sesuai dengan Standar Nasional Indonesia (SNI. Selain rencana tersebut, ditemukan kurangnya kesadaran dari masing-masing staff PTI PDAM Tirta Moedal Kota Semarang terkait dengan keamanan informasi menyebabkan terjadinya berbagai masalah salah satunya penyalahgunaan wewenang yang dilakukan oleh staff PTI yaitu dengan mengubah sistem operasi yang telah ditetapkan oleh PTI PDAM dan kejadian tersebut berdampak pada proses bisnis. Ditemukan pula bahwa kurangnya pengamanan pada ruang server yang ada di divisi PTI. Tujuan dari penelitian ini adalah untuk mengevaluasi tingkat kelengkapan dan tingkat kematangan keamanan informasi pada divisi PTI serta memberikan rekomendasi perbaikan untuk meningkatkan tingkat kelengkapan dan tingkat kematangan keamanan informasi. Dari hasil studi dokumen, wawancara, dan kuesioner berdasarkan Indeks Keamanan Informasi SNI ISO/IEC 27001:2009 dihasilkan tingkat kelengkapan keamanan informasi sebesar 325 dan tingkat kematangan berada pada tingkat I+ dimana sudah ada pemahaman mengenai perlunya pengelolaan kemanan informasi akan tetapi penerapan langkah pengamanan masih belum teratur. Untuk mencapai tingkat kematangan V dan sesuai dengan Standar Nasional Indonesia (SNI PTI PDAM Tirta Moedal Kota Semarang dapat melakukan strategi perbaikan secara bertahap. Kata Kunci: Indeks Keamanan Informasi, SNI ISO/IEC 27001:2009, Evaluasi Keamanan Informasi, Tingkat Kelengkapan, Tingkat Kematangan

  10. NON-DESTRUCTIVE TESTS OF LOCK TONGUES USED IN ATR-72 AIRCRAFT LANDING GEAR BASED ON MAGNETIC METHOD

    Directory of Open Access Journals (Sweden)

    Mirosław Malec

    2013-12-01

    Full Text Available The purpose of this work is to highlight the opportunities of using and analyzing process progression of Non-destructive Testing in aeronautical industries and technologies. This paper concentrates on magnetic-fluorescent method, which is used to showcase the practical test of lock tongue installed in ATR-72 aircraft landing gear.

  11. NON-DESTRUCTIVE TESTS OF LOCK TONGUES USED IN ATR-72 AIRCRAFT LANDING GEAR BASED ON MAGNETIC METHOD

    OpenAIRE

    Mirosław Malec; Tomasz Cieplak; Sławomir Walczuk

    2013-01-01

    The purpose of this work is to highlight the opportunities of using and analyzing process progression of Non-destructive Testing in aeronautical industries and technologies. This paper concentrates on magnetic-fluorescent method, which is used to showcase the practical test of lock tongue installed in ATR-72 aircraft landing gear.

  12. CO Adsorption on Pt Nanoparticles in Low E-Fields Studied by ATR-IR Spectroscopy in a Microreactor

    NARCIS (Netherlands)

    Susarrey Arce, A.; Tiggelaar, R.M.; Gardeniers, J.G.E.; Houselt, van A.; Lefferts, L.

    2015-01-01

    The adsorption of carbon monoxide (CO) on platinum (Pt) nanoparticles under the influence of a low electrical field (E-field) is studied. The adsorption of CO is monitored by attenuated total reflection infrared (ATR-IR) spectroscopy, while an external electrical field is applied over the microreact

  13. MgAtr7, a new type of ABC transporter from Mycosphaerella graminicola involved in iron homeostasis

    NARCIS (Netherlands)

    Zwiers, L.H.; Roohparvar, R.; Waard, de M.A.

    2007-01-01

    The ABC transporter-encoding gene MgAtr7 from the wheat pathogen Mycosphaerella graminicola was cloned based upon its high homology to ABC transporters involved in azole-fungicide sensitivity. Genomic and cDNA sequences indicated that the N-terminus of this ABC transporter contains a motif character

  14. NICT/ATR Chinese-Japanese-English Speech-to-Speech Translation System

    Institute of Scientific and Technical Information of China (English)

    Tohru Shimizu; Yutaka Ashikari; Eiichiro Sumita; ZHANG Jinsong; Satoshi Nakamura

    2008-01-01

    This paper describes the latest version of the Chinese-Japanese-English handheld speech-to-speech translation system developed by NICT/ATR,which is now ready to be deployed for travelers.With the entire speech-to-speech translation function being implemented into one terminal,it realizes real-time,location-free speech-to-speech translation.A new noise-suppression technique notably improves the speech recognition performance.Corpus-based approaches of speech recognition,machine translation,and speech synthesis enable coverage of a wide variety of topics and portability to other languages.Test results show that the character accuracy of speech recognition is 82%-94% for Chinese speech,with a bilingual evaluation understudy score of machine translation is 0.55-0.74 for Chinese-Japanese and Chinese-English.

  15. Interaction of two different types of membrane proteins with model membranes investigated by FTIR ATR spectroscopy

    Science.gov (United States)

    Siam, M.; Reiter, G.; Schwarzott, M.; Baurecht, D.; Fringeli, U. P.

    1998-06-01

    Polarized FTIR ATR spectroscopy was used to investigate the interaction of mitochondrial creatine kinase (Mi-CK) and intestinal alkaline phosphatase (AP) with model membrane assemblies. Mi-CK was immobilized by adsorption to the negatively charged cardiolipin (CL) leaflet of a supported CL/DPPA bilayer. H-D-exchange of the enzyme and the stability under flowthrough conditions of the protein/membrane assembly were examined. AP, however, was bound to a DPPA Langmuir-Blodgett layer (LBL), followed by the completion of a bilayer-like structure by adsorption of POPC molecules from a vesicular solution. It turned out that the POPC adsorbate exhibited decreased molecular order compared to the POPC molecules on a supported POPC/DPPA bilayer. Enzymatic activity of immobilized AP was determined with p-nitrophenyl phosphate (p-NPP) as substrate and remained unchanged for at least 2 days.

  16. Unidirectional excitation of graphene plasmon in attenuated total reflection (ATR) configuration

    Energy Technology Data Exchange (ETDEWEB)

    Dai, Wei [Hubei University of Education, Wuhan (China). School of Physics and Mechanical and Electrical Engineering; Wu, Yue-Chao [Nanyang Technological University, Singapore (Singapore). Div. of Physics and Applied Physics; Liu, Fang-Li [Maryland Univ., College Park, MD (United States). Joint Quantum Institute

    2016-08-01

    Graphene plasmon has been attracting interests from both theoretical and experimental research due to its gate tunability and potential applications in the terahertz frequency range. Here, we propose an effective scheme to unidirectionally excite the graphene plasmon by exploiting magneto-optical materials in the famous attenuated total reflection (ATR) configuration. We show that the graphene plasmon dispersion relation in such a device is asymmetric in different exciting directions, thus making it possible to couple the incident light unidirectionally to the propagating plasmon. The split of absorption spectrum of graphene clearly indicates that under a magnetic field for one single frequency, graphene plasmon can only be excited in one direction. The possible gate tunablity of excitation direction and the further application of the proposed scheme, such as optical isolator, also are discussed.

  17. Synergistic potentiation of (-)-lomaiviticin A cytotoxicity by the ATR inhibitor VE-821.

    Science.gov (United States)

    Colis, Laureen C; Herzon, Seth B

    2016-07-01

    (-)-Lomaiviticin A (1) is a cytotoxic bacterial metabolite that induces double-strand breaks in DNA. Here we show that the cytotoxicity of (-)-lomaiviticin A (1) is synergistically potentiated in the presence of VE-821 (7), an inhibitor of ataxia telangiectasia and Rad3-related protein (ATR). While 0.5nM 1 or 10μM 7 alone are non-lethal to K562 cells, co-incubation of the two leads to high levels of cell kill (81% and 94% after 24 and 48h, respectively). Mechanistic data indicate that cells treated with 1 and 7 suffer extensive DNA double-strand breaks and apoptosis. These data suggest combinations of 1 and 7 may be a valuable chemotherapeutic strategy. PMID:27177826

  18. Analysis of H2O in silicate glass using attenuated total reflectance (ATR) micro-FTIR spectroscopy

    Science.gov (United States)

    Lowenstern, Jacob B.; Pitcher, Bradley W.

    2013-01-01

    We present a calibration for attenuated total reflectance (ATR) micro-FTIR for analysis of H2O in hydrous glass. A Ge ATR accessory was used to measure evanescent wave absorption by H2O within hydrous rhyolite and other standards. Absorbance at 3450 cm−1 (representing total H2O or H2Ot) and 1630 cm−1 (molecular H2O or H2Om) showed high correlation with measured H2O in the glasses as determined by transmission FTIR spectroscopy and manometry. For rhyolite, wt%H2O=245(±9)×A3450-0.22(±0.03) and wt%H2Om=235(±11)×A1630-0.20(±0.03) where A3450 and A1630 represent the ATR absorption at the relevant infrared wavelengths. The calibration permits determination of volatiles in singly polished glass samples with spot size down to ~5 μm (for H2O-rich samples) and detection limits of ~0.1 wt% H2O. Basaltic, basaltic andesite and dacitic glasses of known H2O concentrations fall along a density-adjusted calibration, indicating that ATR is relatively insensitive to glass composition, at least for calc-alkaline glasses. The following equation allows quantification of H2O in silicate glasses that range in composition from basalt to rhyolite: wt%H2O=(ω×A3450/ρ)+b where ω = 550 ± 21, b = −0.19 ± 0.03, ρ = density, in g/cm3, and A3450 is the ATR absorbance at 3450 cm−1. The ATR micro-FTIR technique is less sensitive than transmission FTIR, but requires only a singly polished sample for quantitative results, thus minimizing time for sample preparation. Compared with specular reflectance, it is more sensitive and better suited for imaging of H2O variations in heterogeneous samples such as melt inclusions. One drawback is that the technique can damage fragile samples and we therefore recommend mounting of unknowns in epoxy prior to polishing. Our calibration should hold for any Ge ATR crystals with the same incident angle (31°). Use of a different crystal type or geometry would require measurement of several H2O-bearing standards to provide a crystal

  19. HPV 5 and 8 E6 abrogate ATR activity resulting in increased persistence of UVB induced DNA damage.

    Directory of Open Access Journals (Sweden)

    Nicholas A Wallace

    Full Text Available The role of the E6 oncoprotein from high-risk members of the α human papillomavirus genus in anogenital cancer has been well established. However, far less is known about the E6 protein from the β human papillomavirus genus (β-HPVs. Some β-HPVs potentially play a role in non-melanoma skin cancer development, although they are not required for tumor maintenance. Instead, they may act as a co-factor that enhances the carcinogenic potential of UV damage. Indeed, the E6 protein from certain β-HPVs (HPV 5 and 8 promotes the degradation of p300, a histone acetyl transferase involved in UV damage repair. Here, we show that the expression of HPV 5 and 8 E6 increases thymine dimer persistence as well as the likelihood of a UVB induced double strand break (DSB. Importantly, we provide a mechanism for the increased DNA damage by showing that both extended thymine dimer persistence as well as elevated DSB levels are dependent on the ability of HPV 8 E6 to promote p300 degradation. We further demonstrate that HPV 5 and 8 E6 expression reduces the mRNA and protein levels of ATR, a PI3 kinase family member that plays a key role in UV damage signaling, but that these levels remain unperturbed in cells expressing a mutated HPV 8 E6 incapable of promoting p300 degradation. We confirm that the degradation of p300 leads to a reduction in ATR protein levels, by showing that ATR levels rebound when a p300 mutant resistant to HPV 8 mediated degradation and HPV 8 E6 are co-transfected. Conversely, we show that ATR protein levels are reduced when p300 is targeted for degradation by siRNA. Moreover, we show the reduced ATR levels in HPV 5 and 8 E6 expressing cells results in delayed ATR activation and an attenuated ability of cells to phosphorylate, and as a result accumulate, p53 in response to UVB exposure, leading to significantly reduced cell cycle arrest. In conclusion, these data demonstrate that β-HPV E6 expression can enhance the carcinogenic potential of

  20. Cutaneous approach towards clinical and pathophysiological aspects of hyperglycemia by ATR FTIR spectroscopy

    Science.gov (United States)

    Eikje, Natalja Skrebova; Sota, Takayuki; Aizawa, Katsuo

    2007-07-01

    Attempts were made to non-invasively detect glucose-specific spectral signals in the skin by ATR-FTIR spectroscopy. In vivo spectra were collected from the inner wrists of healthy, prediabetes and diabetes subjects in the 750-4000 cm -1 region, with a closer assessment of the glucose-related region between 1000 and 1180 cm -1. Spectra in vivo showed glucose-specific peaks at 1030, 1080, 1118 and 1151 cm -1, as a variety of glucose solutions are found in vitro. Based on the differences of intensities at 1030 and 1118 cm -1 two spectral patterns were seen: I 1118 > I 1030 for a diabetes and I 1030> I 1118 for non-diabetes subjects. The peak at 1030 cm -1 was used to assess glucose concentrations in the skin due to its good correlation with glucose concentrations in vitro. Calculated mean values of the peak at 1030 cm -1 showed evidence of correlation with blood glucose levels when grouped as = 200 mg/dL, though there was no constant correlation between them when compared before/after OGTT or at the fasting/postprandial states. Absorbances at 1030 cm -1 were not only increased in a dose-dependent manner in a diabetes patient, but were also generally higher than in non-diabetes subjects at 30 min OGTT assessment. Also we could monitor absorbances at 1030 cm -1 and determine their changes in the skin tissue at different times of OGTT. We assume that our approach to in vivo measurement and monitoring of glucose concentrations at 1030 cm -1 may be one of the indicators to assess glucose activity level and its changes in the skin tissue, and has further implications in the study of clinical and pathophysiological aspects of hyperglycemia in diabetes and non-diabetes subjects by ATR-FTIR spectroscopy.

  1. Study of consumer fireworks post-blast residues by ATR-FTIR.

    Science.gov (United States)

    Martín-Alberca, Carlos; Zapata, Félix; Carrascosa, Héctor; Ortega-Ojeda, Fernando E; García-Ruiz, Carmen

    2016-03-01

    Specific analytical procedures are requested for the forensic analysis of pre- and post-blast consumer firework samples, which present significant challenges. Up to date, vibrational spectroscopic techniques such as Fourier transform infrared spectroscopy (FTIR) have not been tested for the analysis of post-blast residues in spite of their interesting strengths for the forensic field. Therefore, this work proposes a simple and fast procedure for the sampling and analysis of consumer firework post-blast residues by a portable FTIR instrument with an Attenuated Total Reflection (ATR) accessory. In addition, the post-blast residues spectra of several consumer fireworks were studied in order to achieve the identification of their original chemical compositions. Hence, this work analysed 22 standard reagents usually employed to make consumer fireworks, or because they are related to their combustion products. Then, 5 different consumer fireworks were exploded, and their residues were sampled with dry cotton swabs and directly analysed by ATR-FTIR. In addition, their pre-blast fuses and charges were also analysed in order to stablish a proper comparison. As a result, the identification of the original chemical compositions of the post-blast samples was obtained. Some of the compounds found were potassium chlorate, barium nitrate, potassium nitrate, potassium perchlorate or charcoal. An additional study involving chemometric tools found that the results might greatly depend on the swab head type used for the sampling, and its sampling efficiency. The proposed procedure could be used as a complementary technique for the analysis of consumer fireworks post-blast residues. PMID:26717839

  2. Rapid detection of NBOME's and other NPS on blotter papers by direct ATR-FTIR spectrometry.

    Science.gov (United States)

    Coelho Neto, José

    2015-07-01

    Blotter paper is among the most common forms of consumption of new psychotropic substances (NPS), formerly referred as designer drugs. In many cases, users are misled to believe they are taking LSD when, in fact, they are taking newer and less known drugs like the NBOMEs or other substituted phenethylamines. We report our findings in quick testing of blotter papers for illicit substances like NBOMEs and other NPS by taking ATR-FTIR spectra directly from blotters seized on the streets, without any sample preparation. Both sides (front and back) of each blotter were tested. Collected data were analyzed by single- and multi-component spectral matching and submitted to chemometric discriminant analysis. Our results showed that, on 66.7% of the cases analyzed, seized blotters contained one or more types of NBOMEs, confirming the growing presence of this novel substances on the market. Matching IR signals were detected on both or just one side of the blotters and showed variable strength. Although no quantitative analysis was made, detection of these substances by the proposed approach serves as indication of variable and possibly higher dosages per blotter when compared to LSD, which showed to be below the detection limit of the applied method. Blotters containing a mescaline-like compound, later confirmed by GC-MS and LC-MS to be MAL (methallylescaline), a substance very similar to mescaline, were detected among the samples tested. Validity of direct ATR-FTIR testing was confirmed by checking the obtained results against independent GC-MS or LC-MS results for the same cases/samples.

  3. A testbed for architecture and fidelity trade studies in the Bayesian decision-level fusion of ATR products

    Science.gov (United States)

    Erickson, Kyle J.; Ross, Timothy D.

    2007-04-01

    Decision-level fusion is an appealing extension to automatic/assisted target recognition (ATR) as it is a low-bandwidth technique bolstered by a strong theoretical foundation that requires no modification of the source algorithms. Despite the relative simplicity of decision-level fusion, there are many options for fusion application and fusion algorithm specifications. This paper describes a tool that allows trade studies and optimizations across these many options, by feeding an actual fusion algorithm via models of the system environment. Models and fusion algorithms can be specified and then exercised many times, with accumulated results used to compute performance metrics such as probability of correct identification. Performance differences between the best of the contributing sources and the fused result constitute examples of "gain." The tool, constructed as part of the Fusion for Identifying Targets Experiment (FITE) within the Air Force Research Laboratory (AFRL) Sensors Directorate ATR Thrust, finds its main use in examining the relationships among conditions affecting the target, prior information, fusion algorithm complexity, and fusion gain. ATR as an unsolved problem provides the main challenges to fusion in its high cost and relative scarcity of training data, its variability in application, the inability to produce truly random samples, and its sensitivity to context. This paper summarizes the mathematics underlying decision-level fusion in the ATR domain and describes a MATLAB-based architecture for exploring the trade space thus defined. Specific dimensions within this trade space are delineated, providing the raw material necessary to define experiments suitable for multi-look and multi-sensor ATR systems.

  4. Quantitative Analysis of the Relative Transcript Levels of ABC Transporter Atr Genes in Aspergillus nidulans by Real-Time Reverse Transcription-PCR Assay

    Science.gov (United States)

    Pizeta Semighini, Camile; Marins, Mozart; Goldman, Maria Helena S.; Goldman, Gustavo Henrique

    2002-01-01

    The development of assays for quantitative analysis of the relative transcript levels of ABC transporter genes by real-time reverse transcription-PCR (RT-PCR) might provide important information about multidrug resistance in filamentous fungi. Here, we evaluate the potential of real-time RT-PCR to quantify the relative transcript levels of ABC transporter Atr genes from Aspergillus nidulans. The AtrA to AtrD genes showed different and higher levels in the presence of structurally unrelated drugs, such as camptothecin, imazalil, itraconazole, hygromycin, and 4-nitroquinoline oxide. We also verified the relative transcript levels of the Atr genes in the A. nidulans imazalil-resistant mutants. These genes displayed a very complex pattern in different ima genetic backgrounds. The imaB mutant has higher basal transcript levels of AtrB and -D than those of the wild-type strain. The levels of these two genes are comparable when the imaB mutant is grown in the presence and absence of imazalil. The imaC, -D, and -H mutants have higher basal levels of AtrA than that of the wild type. The same behavior is observed for the relative transcript levels of AtrB in the imaG mutant background. PMID:11872487

  5. Differential Processing of Low and High LET Radiation Induced DNA Damage: Investigation of Switch from ATM to ATR Signaling

    Science.gov (United States)

    Saha, Janapriya; Wang, Minli; Hada, Megumi; Cucinotta, Francis A.

    2011-01-01

    The members of the phosphatidylinositol kinase-like kinase family of proteins namely ataxia-telangiectasia mutated (ATM) and ATM- and Rad3-related (ATR) are directly responsible for the maintenance of genomic integrity by mounting DDR through signaling and facilitating the recruitment of repair factors at the sites of DNA damage along with coordinating the deployment of cell cycle checkpoints to permit repair by phosphorylating Checkpoint kinase Chk1, Chk2 and p53. High LET radiation from GCR (Galactic Cosmic Rays) consisting mainly of protons and high energy and charged (HZE) particles from SPE (Solar Particle Event) pose a major health risk for astronauts on their space flight missions. The determination of these risks and the design of potential safeguards require sound knowledge of the biological consequences of lesion induction and the capability of the cells to counter them. We here strive to determine the coordination of ATM and ATR kinases at the break sites directly affecting checkpoint signaling and DNA repair and whether differential processing of breaks induced by low and high LET radiation leads to possible augmentation of swap of these damage sensors at the sites of DNA damage. Exposure of cells to IR triggers rapid autophosphorylation of serine-1981 that causes dimer dissociation and initiates monomer formation of ATM. ATM kinase activity depends on the disruption of the dimer, which allows access and phosphorylation of downstream ATM substrates like Chk2. Evidence suggests that ATM is activated by the alterations in higher-order chromatin structure although direct binding of ATM to DSB ends may be a crucial step in its activation. On the other hand, in case of ATR, RPA (replication protein A)-coated ssDNA (single-stranded DNA) generated as a result of stalled DNA replication or during processing of chromosomal lesions is crucial for the localization of ATR to sites of DNA damage in association with ATR-interacting protein (ATRIP). Although the

  6. Quantitative Analysis of the Relative Transcript Levels of ABC Transporter Atr Genes in Aspergillus nidulans by Real-Time Reverse Transcription-PCR Assay

    OpenAIRE

    Pizeta Semighini, Camile; Marins, Mozart; Goldman, Maria Helena S.; Goldman, Gustavo Henrique

    2002-01-01

    The development of assays for quantitative analysis of the relative transcript levels of ABC transporter genes by real-time reverse transcription-PCR (RT-PCR) might provide important information about multidrug resistance in filamentous fungi. Here, we evaluate the potential of real-time RT-PCR to quantify the relative transcript levels of ABC transporter Atr genes from Aspergillus nidulans. The AtrA to AtrD genes showed different and higher levels in the presence of structurally unrelated dr...

  7. Status Report on the Fabrication of Fuel Cladding Chemical Interaction Test Articles for ATR Irradiations

    Energy Technology Data Exchange (ETDEWEB)

    Field, Kevin G. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Howard, Richard H. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2015-09-28

    FeCrAl alloys are a promising new class of alloys for light water reactor (LWR) applications due to their superior oxidation and corrosion resistance in high temperature environments. The current R&D efforts have focused on the alloy composition and processing routes to generate nuclear grade FeCrAl alloys with optimized properties for enhanced accident tolerance while maintaining properties needed for normal operation conditions. Therefore, the composition and processing routes must be optimized to maintain the high temperature steam oxidation (typically achieved by increasing the Cr and Al content) while still exhibiting properties conducive to normal operation in a LWR (such as radiation tolerance where reducing Cr content is favorable). Within this balancing act is the addition of understanding the influence on composition and processing routes on the FeCrAl alloys for fuel-cladding chemical interactions (FCCI). Currently, limited knowledge exists on FCCI for the FeCrAl-UO2 clad-fuel system. To overcome the knowledge gaps on the FCCI for the FeCrAl-UO2 clad-fuel system a series of fueled irradiation tests have been developed for irradiation in the Advanced Test Reactor (ATR) housed at the Idaho National Laboratory (INL). The first series of tests has already been reported. These tests used miniaturized 17x17 PWR fuel geometry rodlets of second-generation FeCrAl alloys fueled with industrial Westinghouse UO2 fuel. These rodlets were encapsulated within a stainless steel housing.To provide high fidelity experiments and more robust testing, a new series of rodlets have been developed deemed the Accident Tolerant Fuel Experiment #1 Oak Ridge National Laboratory FCCI test (ATF-1 ORNL FCCI). The main driving factor, which is discussed in detail, was to provide a radiation environment where prototypical fuel-clad interface temperatures are met while still maintaining constant contact between industrial fuel and the candidate cladding alloys

  8. Blood glucose measurement in vivo using hollow-fiber based, mid-infrared ATR probe with multi-reflection prism

    Science.gov (United States)

    Kino, Saiko; Omori, Suguru; Matsuura, Yuji

    2016-03-01

    An attenuated-total-reflection (ATR), mid-infrared spectroscopy system that consists of hollow optical fibers, a trapezoidal multi-reflection ATR prism, and a conventional FT-IR spectrometer has been developed to measure blood glucose levels. Owing to the low transmission loss and high flexibility of the hollow-optical fiber, the system can measure any sites of the human body where blood capillaries are close to the surface of mucosa, such as inner lips. Using a multi-reflection prism brought about higher sensitivity, and the flat and wide contact surface of the prism resulted in higher measurement reproducibility. The results of in-vivo measurement of human inner lips showed the feasibility of the proposed system, and the measurement errors were within 20%.

  9. Historical perspective and modern applications of Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR).

    Science.gov (United States)

    Blum, Marc-Michael; John, Harald

    2012-01-01

    Vibrational spectroscopy has a long history as an important spectroscopic method in chemical and pharmaceutical analysis. Instrumentation for infrared (IR) spectroscopy was revolutionized by the introduction of Fourier Transform Infrared (FTIR) spectrometers. In addition, easier sampling combined with better sample-to-sample reproducibility and user-to-user spectral variation became available with attenuated total reflectance (ATR) probes and their application for in situ IR spectroscopy. These innovations allow many new applications in chemical and pharmaceutical analysis, such as the use of IR spectroscopy in Process Analytical Chemistry (PAC), the quantitation of drugs in complex matrix formulations, the analysis of protein binding and function and in combination with IR microscopy to the emergence of IR imaging technologies. The use of ATR-FTIR instruments in forensics and first response to 'white powder' incidents is also discussed. A short overview is given in this perspective article with the aim to renew and intensify interest in IR spectroscopy.

  10. Electrooxidation of ethanol on Pt and PtRu surfaces investigated by ATR surface-enhanced infrared absorption spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Marcio F.; Camara, Giuseppe A., E-mail: giuseppe.silva@ufms.br [Departamento de Quimica, Universidade Federal do Mato Grosso do Sul, Campo Grande-MS (Brazil); Batista, Bruno C.; Boscheto, Emerson [Instituto de Quimica de Sao Carlos, Universidade de Sao Paulo, Sao Carlos-SP, (Brazil); Varela, Hamilton, E-mail: varela@iqsc.usp.br [Ertl Center for Electrochemistry and Catalysis, Gwangju Institute of Science and Technology (GIST), Gwangju (Korea, Republic of)

    2012-05-15

    Herein, it was investigated for the first time the electro-oxidation of ethanol on Pt and PtRu electrodeposits in acidic media by using in situ surface enhanced infrared absorption spectroscopy with attenuated total reflection (ATR-SEIRAS). The experimental setup circumvents the weak absorbance signals related to adsorbed species, usually observed for rough, electrodeposited surfaces, and allows a full description of the CO coverage with the potential for both catalysts. The dynamics of adsorption-oxidation of CO was accessed by ATR-SEIRAS experiments (involving four ethanol concentrations) and correlated with expressions derived from a simple kinetic model. Kinetic analysis suggests that the growing of the CO adsorbed layer is nor influenced by the presence of Ru neither by the concentration of ethanol. The results suggest that the C-C scission is not related to the presence of Ru and probably happens at Pt sites. (author)

  11. ATR-FT-IR spectroscopy in the region of 500-230 cm -1 for identification of inorganic red pigments

    Science.gov (United States)

    Vahur, Signe; Knuutinen, Ulla; Leito, Ivo

    2009-08-01

    It is demonstrated that micro-ATR-FT-IR in the low wave number range (500-230 cm -1) can be well used for identification of pigments in paint samples thereby markedly extending the possibilities of pigment identification by ATR-IR spectroscopy into the realm of pigments having no absorptions in the mid-IR region. Reference spectra of pigments can be conveniently obtained by mixing them with linseed oil in approximately 1:1 mass ratio. Vermilion (or cinnabar), read lead, different red iron oxide pigments and cadmium red can be identified. In some cases the method can be used alone for pigment identification and in many cases it provides useful additional evidence for pigment identification using other instrumental techniques (electron microprobe analysis, XRF, optical microscopy).

  12. On-line reaction monitoring of lithiation of halogen substituted acetanilides via in situ calorimetry, ATR spectroscopy, and endoscopy.

    Science.gov (United States)

    Godany, Tamas A; Neuhold, Yorck-Michael; Hungerbühler, Konrad

    2011-01-01

    Lithiation of N-(4-chlorophenyl)-pivalamide (NCP) and two additional substituted acetanilides: 4-fluoroacetanilide (4-F) and 4-chloroacetanilide (4-Cl) has been monitored by means of calorimetry, on-line ATR-IR and UV/vis spectroscopy and endoscopy. The combined on-line monitoring revealed the differences between the reaction paths of the chosen substrates. Thus the product structure and the reaction times for the individual reaction steps can be determined in situ.

  13. AGR-2 Final Data Qualification Report for U.S. Capsules - ATR Cycles 147A Through 154B

    Energy Technology Data Exchange (ETDEWEB)

    Pham, Binh T. [Idaho National Lab. (INL), Idaho Falls, ID (United States). Very High-Temperature Reactor Technology Development Office; Einerson, Jeffrey J. [Idaho National Lab. (INL), Idaho Falls, ID (United States). Very High-Temperature Reactor Technology Development Office

    2014-07-01

    This report provides the data qualification status of AGR-2 fuel irradiation experimental data in four U.S. capsules from all 15 Advanced Test Reactor (ATR) Cycles 147A, 148A, 148B, 149A, 149B, 150A, 150B, 151A, 151B, 152A, 152B, 153A, 153B, 154A, and 154B, as recorded in the Nuclear Data Management and Analysis System (NDMAS). Thus, this report covers data qualification status for the entire AGR-2 irradiation and will replace four previously issued AGR-2 data qualification reports (e.g., INL/EXT-11-22798, INL/EXT-12-26184, INL/EXT-13-29701, and INL/EXT-13-30750). During AGR-2 irradiation, two cycles, 152A and 153A, occurred when the ATR core was briefly at low power, so AGR-2 irradiation data are not used for physics and thermal calculations. Also, two cycles, 150A and 153B, are Power Axial Locator Mechanism (PALM) cycles when the ATR power is higher than during normal cycles. During the first PALM cycle, 150A, the experiment was temporarily moved from the B-12 location to the ATR water canal and during the second PALM cycle, 153B, the experiment was temporarily moved from the B-12 location to the I-24 location to avoid being overheated. During the “Outage” cycle, 153A, seven flow meters were installed downstream from seven Fission Product Monitoring System (FPMS) monitors to measure flows from the monitors and these data are included in the NDMAS database.

  14. Determination of drug content in semisolid formulations by non-invasive spectroscopic methods: FTIR - ATR, - PAS, - Raman and PDS

    Energy Technology Data Exchange (ETDEWEB)

    Gotter, B; Hein, J; Neubert, R H H [Institute of Pharmacy, Martin-Luther-University, Wolfgang-Langenbeck-Str. 4, 06120 Halle/Saale (Germany); Faubel, W; Heissler, St, E-mail: bernhard.gotter@pharmazie.uni-halle.d [Institute of Functional Interfaces, Karlsruhe Institute of Technology, Herrmann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen (Germany)

    2010-03-01

    This study elucidates the potential use of photothermal deflection spectroscopy (PDS), FTIR photoacoustic (FTIR-PAS), FT Raman, and FTIR-attenuated total reflection (FTIR-ATR) spectroscopy as analytical tools for investigating the drug content in semisolid formulations. Regarding the analytical parameters, this study demonstrates the photothermal beam deflection to be definitely comparable to well established spectroscopic methods for this purpose. The correlation coefficients range from 0.990 to 0.999. Likewise, repeatability and limit of detection are comparable.

  15. In situ ATR-FTIR study of the early stages of fly ash geopolymer gel formation.

    Science.gov (United States)

    Rees, Catherine A; Provis, John L; Lukey, Grant C; van Deventer, Jannie S J

    2007-08-14

    The kinetics of geopolymer formation are monitored using a novel in situ attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopic technique. Reaction rates are determined from the intensity variation of the bands related to the geopolymer gel network and the unreacted fly ash particles. Comparison with deuterated geopolymer samples provides critical information regarding peak assignments. An initial induction (lag) period is observed to occur for hydroxide-activated geopolymers, followed by gel evolution according to an approximately linear reaction profile. The length of the lag period is reduced by increasing the concentration of NaOH. An increase in the rate of network formation also occurs with increasing NaOH concentration up to a maximum point, beyond which an increased NaOH concentration leads to a reduced rate of network formation. This trend is attributed to the competing effects of increased alkalinity and stronger ion pairing with an increase in NaOH concentration. In situ analysis also shows that the rate of fly ash dissolution is similar for all moderate- to high-alkali geopolymer slurries, which is attributed to the very highly water-deficient nature of these systems and is contrary to predictions from classical glass dissolution chemistry. This provides for the first time detailed kinetic information describing fly ash geopolymer formation kinetics.

  16. ATR-FTIR spectroscopic characterization of coexisting carbonate surface complexes on hematite

    Science.gov (United States)

    Bargar, J.R.; Kubicki, J.D.; Reitmeyer, R.; Davis, J.A.

    2005-01-01

    The speciation of carbonate adsorbed to hematite in air-equilibrated aqueous solutions has been studied using ATR-FTIR spectroscopy. Samples were measured over a range of pH conditions, at 0.1 M NaCl and at low ionic strength, and in H2O and D2O solutions to permit a multispecies analysis of the data. Second-derivative analyses and fits to the spectra indicate the presence of two major and two minor surface-bound carbonate species. The two major complexes coexist at near-neutral pH and low ionic strength. One of these two complexes is relatively sensitive to ionic strength, being displaced at 0.1 M NaCl, whereas the other is not. Comparison of experimental to DFT/MO-calculated frequencies suggest these two major species to be (a) a monodentate binuclear inner-sphere carbonate surface complex, and (b) a fully or partially solvated carbonate (CO32-) species that is symmetry broken and appears to reside in the structured vicinal water layers at the hematite-water interface, retained by hydrogen bonding and/or other forces. Minor carbonate complexes include diffuse layer CO32- and an unidentified inner-sphere species. Both of the dominant species observed here are likely to be significant controls of the surface charge and sorptive properties of Fe-oxides. Copyright ?? 2005 Elsevier Ltd.

  17. Dissolution of tablet-in-tablet formulations studied with ATR-FTIR spectroscopic imaging.

    Science.gov (United States)

    Wray, Patrick S; Clarke, Graham S; Kazarian, Sergei G

    2013-03-12

    This work uses ATR-FTIR spectroscopic imaging to study the dissolution of delayed release and pH resistant compressed coating pharmaceutical tablets. Tablets with an inner core and outer shell were constructed using a custom designed compaction cell. The core of the delayed release tablets consisted of hydroxypropyl methylcellulose (HPMC) and caffeine. The shell consisted of microcrystalline cellulose (MCC) and glucose. The core of the pH resistant formulations was an ibuprofen and PEG melt and the shell was constructed from HPMC and a basic buffer. UV/vis spectroscopy was used to monitor the lag-time of drug release and visible optical video imaging was used as a complementary imaging technique with a larger field of view. Two delayed release mechanisms were established. For tablets with soluble shell sections, lag-time was dependent upon rapid shell dissolution. For tablets with less soluble shells, the lag-time was controlled by the rate of dissolution medium ingress through the shell and the subsequent expansion of the wet HPMC core. The pH resistant formulations prevented crystallization of the ibuprofen in the core during dissolution despite an acidic dissolution medium. FTIR imaging produced important information about the physical and chemical processes occurring at the interface between tablet sections during dissolution. PMID:23291036

  18. The degradation potential of PET bottles in the marine environment: An ATR-FTIR based approach

    Science.gov (United States)

    Ioakeimidis, C.; Fotopoulou, K. N.; Karapanagioti, H. K.; Geraga, M.; Zeri, C.; Papathanassiou, E.; Galgani, F.; Papatheodorou, G.

    2016-03-01

    The dominance and persistence of plastic debris in the marine environment are well documented. No information exists in respect to their lifespan in the marine environment. Nevertheless, the degradation potential of plastic litter items remains a critical issue for marine litter research. In the present study, polyethylene terephthalate bottles (PETs) collected from the submarine environment were characterized using ATR-FTIR in respect to their degradation potential attributed to environmental conditions. A temporal indication was used as indicative to the years of presence of the PETs in the environment as debris. PETs seem to remain robust for approximately fifteen years. Afterwards, a significant decrease of the native functional groups was recorded; some even disappear; or new-not typical for PETs-are created. At a later stage, using the PET time series collected from the Saronikos Gulf (Aegean Sea–E. Mediterranean), it was possible to date bottles that were collected from the bottom of the Ionian Sea (W. Greece). It is the first time that such a study has been conducted with samples that were actually degraded in the marine environment.

  19. Probing the Mec1ATR Checkpoint Activation Mechanism with Small Peptides.

    Science.gov (United States)

    Wanrooij, Paulina H; Tannous, Elias; Kumar, Sandeep; Navadgi-Patil, Vasundhara M; Burgers, Peter M

    2016-01-01

    Yeast Mec1, the ortholog of human ATR, is the apical protein kinase that initiates the cell cycle checkpoint in response to DNA damage and replication stress. The basal activity of Mec1 kinase is activated by cell cycle phase-specific activators. Three distinct activators stimulate Mec1 kinase using an intrinsically disordered domain of the protein. These are the Ddc1 subunit of the 9-1-1 checkpoint clamp (ortholog of human and Schizosaccharomyces pombe Rad9), the replication initiator Dpb11 (ortholog of human TopBP1 and S. pombe Cut5), and the multifunctional nuclease/helicase Dna2. Here, we use small peptides to determine the requirements for Mec1 activation. For Ddc1, we identify two essential aromatic amino acids in a hydrophobic environment that when fused together are proficient activators. Using this increased insight, we have been able to identify homologous motifs in S. pombe Rad9 that can activate Mec1. Furthermore, we show that a 9-amino acid Dna2-based peptide is sufficient for Mec1 activation. Studies with mutant activators suggest that binding of an activator to Mec1 is a two-step process, the first step involving the obligatory binding of essential aromatic amino acids to Mec1, followed by an enhancement in binding energy through interactions with neighboring sequences.

  20. The degradation potential of PET bottles in the marine environment: An ATR-FTIR based approach.

    Science.gov (United States)

    Ioakeimidis, C; Fotopoulou, K N; Karapanagioti, H K; Geraga, M; Zeri, C; Papathanassiou, E; Galgani, F; Papatheodorou, G

    2016-01-01

    The dominance and persistence of plastic debris in the marine environment are well documented. No information exists in respect to their lifespan in the marine environment. Nevertheless, the degradation potential of plastic litter items remains a critical issue for marine litter research. In the present study, polyethylene terephthalate bottles (PETs) collected from the submarine environment were characterized using ATR-FTIR in respect to their degradation potential attributed to environmental conditions. A temporal indication was used as indicative to the years of presence of the PETs in the environment as debris. PETs seem to remain robust for approximately fifteen years. Afterwards, a significant decrease of the native functional groups was recorded; some even disappear; or new-not typical for PETs-are created. At a later stage, using the PET time series collected from the Saronikos Gulf (Aegean Sea-E. Mediterranean), it was possible to date bottles that were collected from the bottom of the Ionian Sea (W. Greece). It is the first time that such a study has been conducted with samples that were actually degraded in the marine environment.

  1. The Conference Proceedings of the 2003 Air Transport Research Society (ATRS) World Conference, Volume 5

    Science.gov (United States)

    Bowen, Brent (Editor); Gudmundsson, Sveinn (Editor); Oum, Tae (Editor)

    2003-01-01

    The UNO Aviation Institute Monograph Series began in 1994 as a key component of the education outreach and information transfer missions of the Aviation Institute and the NASA Nebraska Space Grant & EPSCoR Programs. The series is an outlet for aviation materials to be indexed and disseminated through an efficient medium. Publications are welcome in all aspects of aviation. Publication formats may include, but are not limited to, conference proceedings, bibliographies, research reports, manuals, technical reports, and other documents that should be archived and indexed for future reference by the aviation and world wide communities. The Conference proceedings of the 2003 Air Transport Research Society (ATRS) world conference, volume 5 is presented. The topics include: 1) The Temporal Configuration of Airline Networks in Europe; 2) Determination and Applications of Environmental Costs at Different Sized Airports-Aircraft Noise and Engine Emissions; 3) Cost Effective Measures to Reduce CO2 Emissions in the Air Freight Sector; 4) An Assessment of the Sustainability of Air Transport System: Quantification of Indicators; 5) Regulation, Competition and Network Evolution in Aviation; 6) Regulation in the Air: Price and Frequency Cap; 7) Industry Consolidation and Future Airline Network Structures in Europe; 8) Application of Core Theory to the U.S. Airline Industry; 9) Air Freight Transshipment Route Choice Analysis; 10) A Fuzzy Approach of the Competition on Air Transport Market; and 11) Developing Passenger Demand Models for International Aviation from/to Egypt: A Case Study of Cairo Airport and Egyptair.

  2. Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes

    Energy Technology Data Exchange (ETDEWEB)

    Takai, Hiroyuki; Xie, Yihu; de Lange, Titia; Pavletich, Nikola P. (Rockefeller); (SKI)

    2010-09-20

    We reported previously that the stability of all mammalian phosphatidylinositol 3-kinase-related protein kinases (PIKKs) depends on their interaction with Tel2, the ortholog of yeast Tel2 and Caenorhabditis elegans Clk-2. Here we provide evidence that Tel2 acts with Hsp90 in the maturation of PIKK complexes. Quantitative immunoblotting showed that the abundance of Tel2 is low compared with the PIKKs, and Tel2 preferentially bound newly synthesized ATM, ATR, mTOR, and DNA-PKcs. Tel2 complexes contained, in addition to Tti1-Tti2, the Hsp90 chaperone, and inhibition of Hsp90 interfered with the interaction of Tel2 with the PIKKs. Analysis of in vivo labeled nascent protein complexes showed that Tel2 and Hsp90 mediate the formation of the mTOR TORC1 and TORC2 complexes and the association of ATR with ATRIP. The structure of yeast Tel2, reported here, shows that Tel2 consists of HEAT-like helical repeats that assemble into two separate {alpha}-solenoids. Through mutagenesis, we identify a surface patch of conserved residues involved in binding to the Tti1-Tti2 complex in vitro. In vivo, mutation of this conserved patch affects cell growth, levels of PIKKs, and ATM/ATR-mediated checkpoint signaling, highlighting the importance of Tti1-Tti2 binding to the function of Tel2. Taken together, our data suggest that the Tel2-Tti1-Tti2 complex is a PIKK-specific cochaperone for Hsp90.

  3. Image-based ATR utilizing adaptive clutter filter detection, LLRT classification, and Volterra fusion with application to side-looking sonar

    Science.gov (United States)

    Aridgides, Tom; Fernández, Manuel

    2010-04-01

    An improved automatic target recognition (ATR) processing string has been developed. The overall processing string consists of pre-processing, subimage adaptive clutter filtering, detection, feature extraction, optimal subset feature selection, feature orthogonalization and classification processing blocks. The objects that are classified by three distinct ATR strings are fused using the classification confidence values and their expansions as features, and using "summing" or log-likelihood-ratio-test (LLRT) based fusion rules. These three ATR processing strings were individually developed and tuned by researchers from different companies. The utility of the overall processing strings and their fusion was demonstrated with an extensive side-looking sonar dataset. In this paper we describe a new processing improvement: six additional classification features are extracted, using primarily target shadow information and a feature extraction window whose length is now made variable as a function of range. This new ATR processing improvement resulted in a 3:1 reduction in false alarms. Two advanced fusion algorithms are subsequently applied: First, a nonlinear Volterra expansion (2nd order) feature-LLRT fusion algorithm is employed. Second, a repeated application of a subset Volterra feature selection / feature orthogonalization / LLRT fusion block is utilized. It is shown that cascaded Volterra feature- LLRT fusion of the ATR processing strings outperforms baseline "summing" and single-stage Volterra feature-LLRT fusion algorithms, yielding significant improvements over the best single ATR processing string results, and providing the capability to correctly call the majority of targets while maintaining a very low false alarm rate.

  4. Ataxia telangiectasia mutated and Rad3 related (ATR protein kinase inhibition is synthetically lethal in XRCC1 deficient ovarian cancer cells.

    Directory of Open Access Journals (Sweden)

    Rebeka Sultana

    Full Text Available INTRODUCTION: Ataxia telangiectasia mutated and Rad3 Related (ATR protein kinase is a key sensor of single-stranded DNA associated with stalled replication forks and repair intermediates generated during DNA repair. XRCC1 is a critical enzyme in single strand break repair and base excision repair. XRCC1-LIG3 complex is also an important contributor to the ligation step of the nucleotide excision repair response. METHODS: In the current study, we investigated synthetic lethality in XRCC1 deficient and XRCC1 proficient Chinese Hamster ovary (CHO and human ovarian cancer cells using ATR inhibitors (NU6027. In addition, we also investigated the ability of ATR inhibitors to potentiate cisplatin cytotoxicity in XRCC1 deficient and XRCC1 proficient CHO and human cancer cells. Clonogenic assays, alkaline COMET assays, γH2AX immunocytochemistry, FACS for cell cycle as well as FITC-annexin V flow cytometric analysis were performed. RESULTS: ATR inhibition is synthetically lethal in XRCC1 deficient cells as evidenced by increased cytotoxicity, accumulation of double strand DNA breaks, G2/M cell cycle arrest and increased apoptosis. Compared to cisplatin alone, combination of cisplatin and ATR inhibitor results in enhanced cytotoxicity in XRCC1 deficient cells compared to XRCC1 proficient cells. CONCLUSIONS: Our data provides evidence that ATR inhibition is suitable for synthetic lethality application and cisplatin chemopotentiation in XRCC1 deficient ovarian cancer cells.

  5. PERANCANGAN PENILAIAN KINERJA KARYAWAN BERDASARKAN KOMPETENSI SPENCER DENGAN METODE ANALYTICAL HIERARCHY PROCESS(Studi Kasus di Sub Dinas Pengairan Dinas Pekerjaan Umum Kota Probolinggo

    Directory of Open Access Journals (Sweden)

    Eko Nurmianto

    2006-01-01

    Expert Choice. Hasilnya bobot kemampuan karyawan kontrak terbagi atas: kemampuan manajerial (0.200 dan kemampuan teknik (0.800. Kemampuan manajerial terdiri dari: disiplin (0.318, melayani (0.289, berprestasi (0.151, proaktif (0.140, dan komitmen pada organisasi (0.102. Sedangkan kemampuan teknis terdiri dari: memimpin (0.500 dan kerjasama (0.500. Dari penelitian tersebut sistem insentif yang tepat adalah sistem insentif pembagian laba, dimana insentif yang diterima berdasarkan pada peningkatan kinerja karyawan. Kata kunci: penilaian kinerja karyawan, kompetensi, insentif, analytical hierarchy process.

  6. An Approach for Routine Analytical Detection of Beeswax Adulteration Using FTIR-ATR Spectroscopy

    Directory of Open Access Journals (Sweden)

    Svečnjak Lidija

    2015-12-01

    Full Text Available Although beeswax adulteration represents one of the main beeswax quality issues, there are still no internationally standardised analytical methods for routine quality control. The objective of this study was to establish an analytical procedure suitable for routine detection of beeswax adulteration using FTIR-ATR spectroscopy. For the purpose of this study, reference IR spectra of virgin beeswax, paraffin, and their mixtures containing different proportions of paraffin (5 - 95%, were obtained. Mixtures were used for the establishment of calibration curves. To determine the prediction strength of IR spectral data for the share of paraffin in mixtures, the Partial Least Squares Regression method was used. The same procedure was conducted on beeswax-beef tallow mixtures. The model was validated using comb foundation samples of an unknown chemical background which had been collected from the international market (n = 56. Selected physico-chemical parameters were determined for comparison purposes. Results revealed a strong predictive power (R2 = 0.999 of IR spectra for the paraffin and beef tallow share in beeswax. The results also revealed that the majority of the analysed samples (89% were adulterated with paraffin; only 6 out of 56 (11% samples were identified as virgin beeswax, 28% of the samples exhibited a higher level of paraffin adulteration (>46% of paraffin, while the majority of the analysed samples (50% were found to be adulterated with 5 - 20% of paraffin. These results indicate an urgent need for routine beeswax authenticity control. In this study, we demonstrated that the analytical approach defining the standard curves for particular adulteration levels in beeswax, based on chemometric modelling of specific IR spectral region indicative for adulteration, enables reliable determination of the adulterant proportions in beeswax.

  7. Simultaneous inhibition of ATR and PARP sensitizes colon cancer cell lines to irinotecan

    Directory of Open Access Journals (Sweden)

    Atlal eAbu-Sanad

    2015-07-01

    Full Text Available Enhanced DNA damage repair is one mechanism involved in colon cancer drug resistance. Thus, targeting molecular components of repair pathways with specific small molecule inhibitors may improve the efficacy of chemotherapy. ABT-888 and VE-821, inhibitors of poly-ADP-ribose-polymerase (PARP and the serine/threonine-kinase Ataxia telangiectasia related (ATR, respectively, were used to treat colon cancer cell lines in combination with the topoisomerase-I inhibitor irinotecan (SN38. Our findings show that each of these DNA repair inhibitors utilized alone at nontoxic single agent concentrations resulted in sensitization to SN38 producing a 1.4 to 3 fold reduction in the 50% inhibitory concentration (IC50 of SN38 in three colon cancer cell lines. When combined together, nontoxic concentrations of ABT-888 and VE-821 produced a 4.5 to 27 fold reduction in the IC50 of SN38 with the HCT-116 colon cancer cells demonstrating the highest sensitization as compared to LoVo and HT-29 colon cancer cells. Furthermore, the combination of all three agents was associated with maximal G2-M arrest and enhanced DNA-damage (γH2AX in all three colon cancer cell lines. The mechanism of this enhanced sensitization was associated with: (a maximal suppression of SN38 induced PARP activity in the presence of both inhibitors and (b ABT-888 producing partial abrogation of the VE-821 enhancement of SN38 induced DNA-PK phosphorylation, resulting in more unrepaired DNA damage; these alterations were only present in the HCT-116 cells which have reduced levels of ATM. This novel combination of DNA repair inhibitors may be useful to enhance the activity of DNA damaging chemotherapies such as irinotecan and help produce sensitization to this drug in colon cancer.

  8. Surface interactions of aromatic organoarsenical compounds with hematite nanoparticles using ATR-FTIR: kinetic studies.

    Science.gov (United States)

    Arts, Derek; Abdus Sabur, Md; Al-Abadleh, Hind A

    2013-03-14

    Aromatic organoarsenicals p-arsanilic acid (pAsA) and roxarsone (ROX) are used as feed additives in developing countries that allow the use of arsenic-containing compounds in their poultry industry. These compounds are introduced to the environment through the application of contaminated poultry litter. Little is known about the surface chemistry of these organoarsenicals on the molecular level with reactive components in soils. We report herein the first in situ and surface-sensitive rapid kinetic studies on the adsorption and desorption of pAsA to/from hematite nanoparticles at pH 7 using ATR-FTIR. Values for the apparent initial rates of adsorption and desorption were extracted from experimental data as a function of spectral components. Hydrogen phosphate was used as a desorbing agent due to its ubiquitous presence in litter, and its adsorption kinetics was investigated on surfaces with and without surface arsenic. Initial first-order pseudo-adsorption rate constant for pAsA was lower by a factor of 1.6 than that of iAs(V), suggesting an average behavior for the formation of quantitatively more weakly bonded monodentate or hydrogen-bonded complexes for the former relative to strongly bonded bidentate surface complexes for the latter under our experimental conditions. Initial first-order pseudo-adsorption rate constants for hydrogen phosphate decrease in this order: fresh hematite > pAsA/hematite ≈ phenylarsonic acid (PhAs)/hematite > iAs/hematite by factors 1.5 and 3 relative to fresh films, respectively. Initial desorption kinetics of aromatic organoarsenicals due to flowing hydrogen phosphate proceed with a nonunity overall order, suggesting a complex mechanism, which is consistent with the existence of more than one type of surface complexes. The impact of our studies on the environmental fate and transport of aromatic organoarsenicals in geochemical environments and their overall surface chemistry with iron (oxyhyr)oxides is discussed.

  9. Application of FTIR-ATR spectroscopy coupled with multivariate analysis for rapid estimation of butter adulteration.

    Science.gov (United States)

    Fadzlillah, Nurrulhidayah Ahmad; Rohman, Abdul; Ismail, Amin; Mustafa, Shuhaimi; Khatib, Alfi

    2013-01-01

    In dairy product sector, butter is one of the potential sources of fat soluble vitamins, namely vitamin A, D, E, K; consequently, butter is taken into account as high valuable price from other dairy products. This fact has attracted unscrupulous market players to blind butter with other animal fats to gain economic profit. Animal fats like mutton fat (MF) are potential to be mixed with butter due to the similarity in terms of fatty acid composition. This study focused on the application of FTIR-ATR spectroscopy in conjunction with chemometrics for classification and quantification of MF as adulterant in butter. The FTIR spectral region of 3910-710 cm⁻¹ was used for classification between butter and butter blended with MF at various concentrations with the aid of discriminant analysis (DA). DA is able to classify butter and adulterated butter without any mistakenly grouped. For quantitative analysis, partial least square (PLS) regression was used to develop a calibration model at the frequency regions of 3910-710 cm⁻¹. The equation obtained for the relationship between actual value of MF and FTIR predicted values of MF in PLS calibration model was y = 0.998x + 1.033, with the values of coefficient of determination (R²) and root mean square error of calibration are 0.998 and 0.046% (v/v), respectively. The PLS calibration model was subsequently used for the prediction of independent samples containing butter in the binary mixtures with MF. Using 9 principal components, root mean square error of prediction (RMSEP) is 1.68% (v/v). The results showed that FTIR spectroscopy can be used for the classification and quantification of MF in butter formulation for verification purposes.

  10. [Research on Rapid Discrimination of Edible Oil by ATR Infrared Spectroscopy].

    Science.gov (United States)

    Ma, Xiao; Yuan, Hong-fu; Song, Chun-feng; Hu, Ai-qin; Li, Xiao-yu; Zhao, Zhong; Li, Xiu-qin; Guo Zhen; Zhu, Zhi-qiang

    2015-07-01

    A rapid discrimination method of edible oils, KL-BP model, was proposed by attenuated total reflectance infrared spectroscopy. The model extracts the characteristic of classification from source data by KL and reduces data dimension at the same time. Then the neural network model is constructed by the new data which as the input of the model. 84 edible oil samples which include sesame oil, corn oil, canola oil, blend oil, sunflower oil, peanut oil, olive oil, soybean oil and tea seed oil, were collected and their infrared spectra determined using an ATR FT-IR spectrometer. In order to compare the method performance, principal component analysis (PCA) direct-classification model, KL direct-classification model, PLS-DA model, PCA-BP model and KL-BP model are constructed in this paper. The results show that the recognition rates of PCA, PCA-BP, KL, PLS-DA and KL-BP are 59.1%, 68.2%, 77.3%, 77.3% and 90.9% for discriminating the 9 kinds of edible oils, respectively. KL extracts the eigenvector which make the distance between different class and distance of every class ratio is the largest. So the method can get much more classify information than PCA. BP neural network can effectively enhance the classification ability and accuracy. Taking full of the advantages of KL in extracting more category information in dimension reducing and the features of BP neural network in self-learning, adaptive, nonlinear, the KL-BP method has the best classification ability and recognition accuracy and great importance for rapidly recognizing edible oil in practice.

  11. Checkpoint Kinase ATR Promotes Nucleotide Excision Repair of UV-induced DNA Damage via Physical Interaction with Xeroderma Pigmentosum Group A*

    Science.gov (United States)

    Shell, Steven M.; Li, Zhengke; Shkriabai, Nikolozi; Kvaratskhelia, Mamuka; Brosey, Chris; Serrano, Moises A.; Chazin, Walter J.; Musich, Phillip R.; Zou, Yue

    2009-01-01

    In response to DNA damage, eukaryotic cells activate a series of DNA damage-dependent pathways that serve to arrest cell cycle progression and remove DNA damage. Coordination of cell cycle arrest and damage repair is critical for maintenance of genomic stability. However, this process is still poorly understood. Nucleotide excision repair (NER) and the ATR-dependent cell cycle checkpoint are the major pathways responsible for repair of UV-induced DNA damage. Here we show that ATR physically interacts with the NER factor Xeroderma pigmentosum group A (XPA). Using a mass spectrometry-based protein footprinting method, we found that ATR interacts with a helix-turn-helix motif in the minimal DNA-binding domain of XPA where an ATR phosphorylation site (serine 196) is located. XPA-deficient cells complemented with XPA containing a point mutation of S196A displayed a reduced repair efficiency of cyclobutane pyrimidine dimers as compared with cells complemented with wild-type XPA, although no effect was observed for repair of (6-4) photoproducts. This suggests that the ATR-dependent phosphorylation of XPA may promote NER repair of persistent DNA damage. In addition, a K188A point mutation of XPA that disrupts the ATR-XPA interaction inhibits the nuclear import of XPA after UV irradiation and, thus, significantly reduced DNA repair efficiency. By contrast, the S196A mutation has no effect on XPA nuclear translocation. Taken together, our results suggest that the ATR-XPA interaction mediated by the helix-turn-helix motif of XPA plays an important role in DNA-damage responses to promote cell survival and genomic stability after UV irradiation. PMID:19586908

  12. SURVEY DAN ANALISIS SEISMISITAS WILAYAH JAWA TIMUR BERDASARKAN DATA GEMPA BUMI PERIODE 1999-2013 SEBAGAI UPAYA MITIGASI BENCANA GEMPA BUMI

    Directory of Open Access Journals (Sweden)

    Jihan Nia Shohaya

    2013-11-01

    Full Text Available Kajian kegempaan suatu wilayah terutama di daerah rawan gempa bumi seperti Jawa Timur sangat diperlukan dalam rangka mengurangi dampak yang ditimbulkan akibat bencana gempa bumi. Dalam konteks ini, dilakukan penelitian yang bertujuan untuk menganalisis tingkat seismisitas di wilayah Jawa Timur antara tahun 1999-2013 yaitu dengan menganalisis hubungan antara frekuensi kejadian gempa bumi (N dan magnitudo gempa bumi (M. Rumus umum yang digunakan, yaitu log N = a – bM. Gradien persamaan ini atau nilai b merupakan parameter tektonik yang menggambarkan sifat batuan pada daerah yang diteliti sedangkan nilai a menggambarkan aktivitas tektonik wilayah yang diamati. Data yang digunakan dalam penelitian ini merupakan data sekunder parameter gempa bumi dari bank data seismologi IRIS dengan magnitudo 4,0-10,0 SR pada kedalaman 0-200 km yang berlokasi antara 110o-115o BT dan 5o-10o LS. Berdasarkan hasil analisis dengan metode maximum likelihood, didapatkan besarnya parameter seismisitas untuk wilayah 1 diperoleh nilai a sebesar 11,7 dan nilai b sebesar 1,86. Sementara untuk wilayah 2 diperoleh nilai a sebesar 9,69 dan nilai b sebesar 1,49. Hasil analisis menunjukkan bahwa wilayah 1 memiliki tingkat seismitas yang lebih besar dibandingkan wilayah 2. Sedangkan nilai b pada kedua wilayah relatif sama. Hal tersebut menunjukkan bahwa batuan pada wilayah Jawa Timur memiliki heterogenitas yang rendah. Kata kunci : Seismisitas, Gempa Bumi, Jawa Timur Abstract Studying about seismicity pattern, especially in earthquake prone areas such as East Java is needed in order to reduce the impact caused by the earthquake. This research is analyze the level of seismicity and the return period of earthquakes by analyzing the relationship between the frequency of occurrence of earthquakes (N and the earthquake magnitude (M. The main formula is log N = a - bM, gradient equation or b value describes the nature of tectonic rocks of observed regions, and a value describes the

  13. UV-induced nuclear import of XPA is mediated by importin-α4 in an ATR-dependent manner.

    Directory of Open Access Journals (Sweden)

    Zhengke Li

    Full Text Available Xeroderma pigmentosum Group A (XPA is a crucial factor in mammalian nucleotide excision repair (NER and nuclear import of XPA from the cytoplasm for NER is regulated in cellular DNA damage responses in S-phase. In this study, experiments were carried out to determine the transport mechanisms that are responsible for the UV (ultraviolet-induced nuclear import of XPA. We found that, in addition to the nuclear localization signal (NLS of XPA, importin-α4 or/and importin-α7 are required for the XPA nuclear import. Further investigation indicated that, importin-α4 and importin-α7 directly interacted with XPA in cells. Interestingly, the binding of importin-α4 to XPA was dependent on UV-irradiation, while the binding of importin-α7 was not, suggesting a role for importin-α7 in nuclear translocation of XPA in the absence of DNA damage, perhaps with specificity to certain non-S-phases of the cell-cycle. Consistent with the previous report of a dependence of UV-induced XPA nuclear import on ataxia telangiectasia and Rad3-related protein (ATR in S-phase, knockdown of ATR reduced the amount of XPA interacting with importin-α4. In contrast, the GTPase XPA binding protein 1 (XAB1, previously proposed to be required for XPA nuclear import, showed no effect on the nuclear import of XPA in our siRNA knockdown analysis. In conclusion, our results suggest that upon DNA damage transport adaptor importin-α4 imports XPA into the nucleus in an ATR-dependent manner, while XAB1 has no role in this process. In addition, these findings reveal a potential new therapeutic target for the sensitization of cancer cells to chemotherapy.

  14. miR-107 activates ATR/Chk1 pathway and suppress cervical cancer invasion by targeting MCL1.

    Directory of Open Access Journals (Sweden)

    Chengyan Zhou

    Full Text Available MicroRNAs (miRNAs are a class of single-stranded, non-coding RNAs of about 22 nucleotides in length. Increasing evidence implicates miRNAs may function as oncogenes or tumor suppressors. Here we showed that miR-107 directly targeted MCL1 and activated ATR/Chk1 pathway to inhibit proliferation, migration and invasiveness of cervical cancer cells. Moreover, we found that MCL1 was frequently up-regulated in cervical cancer, and knockdown of MCL1 markedly inhibited cancer cell proliferation, migration and invasion, whereas ectopic expression of MCL1 significantly enhances these properties. The restoration of MCL1 expression can counteract the effect of miR-107 on the cancer cells. Together, miR-107 is a new regulator of MCL1, and both miR-107 and MCL1 play important roles in the pathogenesis of cervical cancer. We have therefore identified a mechanism for ATR/Chk1 pathway which involves an increase in miR-107 leading to a decrease in MCL1. Correspondingly, our results revealed that miR-107 affected ATR/Chk1 signalling and gene expression, and implicated miR-107 as a therapeutic target in human cervical cancer. We also demonstrated that taxol attenuated migration and invasion in cervical cancer cells by activating the miR-107, in which miR-107 play an important role in regulating the expression of MCL1. Elucidation of this discovered MCL1 was directly regulated by miR-107 will greatly enhance our understanding of the mechanisms responsible for cervical cancer and will provide an additional arm for the development of anticancer therapies.

  15. Comparison of the Radiosensitizing Effect of ATR, ATM and DNA-PK Kinase Inhibitors on Cervical Carcinoma Cells.

    Science.gov (United States)

    Vávrová, J; Zárybnická, L; Jošt, P; Tichý, A; Řezáčová, M; Šinkorová, Z; Pejchal, J

    2016-01-01

    Here, we compared the effects of inhibitors of three phosphatidylinositol-3-kinase-related kinases, ATM, ATR a DNA-PK, on radiosensitization of cervical carcinoma cells. We demonstrated that DNA-PK inhibitor NU7441 enhanced phosphorylation of Chk1 and Chk2 kinases 2 h after irradiation of HeLa cells at a dose of 8 Gy in contrast to ATM kinase inhibitor KU55933, which completely blocked the Chk2 kinase phosphorylation on threonine 68, and ATR kinase inhibitor VE-821, which blocked the Chk1 kinase phosphorylation on serine 345. Most HeLa cells were accumulated in G2 phase of the cell cycle 24 h after irradiation at a high dose of 15 Gy, which was even potentiated after adding the inhibitors NU7441 and KU55933. Compared to all other irradiated groups, inhibitor VE-821 increased the number of cells in S phase and reduced the number of cells in G2 phase 24 h after irradiation at the high dose of 15 Gy. HeLa cells entered the mitotic cycle with unrepaired DNA, which resulted in cell death and the radiosensitizing effect of VE-821. Short-term application of the inhibitors (2 h before and 30 min after the irradiation by the dose of 8 Gy) significantly decreased the colony-forming ability of HeLa cells. Using real-time monitoring of cell proliferation by the xCELLigence system we demonstrated that while the radiosensitizing effect of VE-821 (ATR inhibitor) is manifested early after the irradiation, the radiosensitizing effect of KU55933 (ATM inhibitor) and NU7441 (DNA-PK inhibitor) is only observed as late as 72 h after the irradiation. PMID:27643582

  16. In situ-ATR-FTIR analysis on the uptake and release of streptomycin from polyelectrolyte complex layers

    Science.gov (United States)

    Torger, B.; Müller, M.

    2013-03-01

    In-situ ATR-FTIR spectroscopy and line shape analysis of the diagnostic spectral region was used to quantify the bound amount and release of the antibiotic streptomycin (STRP) at polyelectrolyte (PEL) multilayers (PEM) of poly(ethyleneimine) (PEI) and poly(acrylic acid) (PAA) or PEI and sodium alginate (ALG). Unlike common concepts based on the drug enrichment of the release medium, this analytical concept allowed to measure quantitatively the drug depletion in the delivery matrix. The measured kinetic in situ ATR-FTIR data were analysed by a modified Korsmeyer-Peppas equation based on two characteristic release parameters k and n. As main experimental parameters the number of PEL layers (adsorption steps) z and the STRP/PEL ratio were varied. For z = 8 the STRP/PEL ratio showed the most significant influence on release kinetics, whereby for STRP/PEL = 1:25 slowest (n = 0.77) and lowest (k = 21.4%) and for STRP/PEL = 1:5 most rapid (n = 0.30) and highest (k = 58.6%) drug releases were found. PEM-PEI/ALG-8 (STRP/PEL = 1:5) revealed slower release rates (n = 0.58) and lower released STRP amounts (k = 17.1%) compared to PEI/PAA. UV-VIS data on time dependent STRP enrichment of the release medium showed a similar trend compared to respective ATR-FTIR data on STRP depletion in PEM. Released amounts of around 1-2 mg from the herein introduced PEM films could be determined. The introduced analytical concept will be used as screening tool for other drugs, drug eluting films and bone substituting materials.

  17. Mutation analysis and characterization of ATR sequence variants in breast cancer cases from high-risk French Canadian breast/ovarian cancer families

    Directory of Open Access Journals (Sweden)

    Pichette Roxane

    2006-09-01

    Full Text Available Abstract Background Ataxia telangiectasia-mutated and Rad3-related (ATR is a member of the PIK-related family which plays, along with ATM, a central role in cell-cycle regulation. ATR has been shown to phosphorylate several tumor suppressors like BRCA1, CHEK1 and TP53. ATR appears as a good candidate breast cancer susceptibility gene and the current study was designed to screen for ATR germline mutations potentially involved in breast cancer predisposition. Methods ATR direct sequencing was performed using a fluorescent method while widely available programs were used for linkage disequilibrium (LD, haplotype analyses, and tagging SNP (tSNP identification. Expression analyses were carried out using real-time PCR. Results The complete sequence of all exons and flanking intronic sequences were analyzed in DNA samples from 54 individuals affected with breast cancer from non-BRCA1/2 high-risk French Canadian breast/ovarian families. Although no germline mutation has been identified in the coding region, we identified 41 sequence variants, including 16 coding variants, 3 of which are not reported in public databases. SNP haplotypes were established and tSNPs were identified in 73 healthy unrelated French Canadians, providing a valuable tool for further association studies involving the ATR gene, using large cohorts. Our analyses led to the identification of two novel alternative splice transcripts. In contrast to the transcript generated by an alternative splicing site in the intron 41, the one resulting from a deletion of 121 nucleotides in exon 33 is widely expressed, at significant but relatively low levels, in both normal and tumoral cells including normal breast and ovarian tissue. Conclusion Although no deleterious mutations were identified in the ATR gene, the current study provides an haplotype analysis of the ATR gene polymorphisms, which allowed the identification of a set of SNPs that could be used as tSNPs for large-scale association

  18. Mutation analysis and characterization of ATR sequence variants in breast cancer cases from high-risk French Canadian breast/ovarian cancer families

    International Nuclear Information System (INIS)

    Ataxia telangiectasia-mutated and Rad3-related (ATR) is a member of the PIK-related family which plays, along with ATM, a central role in cell-cycle regulation. ATR has been shown to phosphorylate several tumor suppressors like BRCA1, CHEK1 and TP53. ATR appears as a good candidate breast cancer susceptibility gene and the current study was designed to screen for ATR germline mutations potentially involved in breast cancer predisposition. ATR direct sequencing was performed using a fluorescent method while widely available programs were used for linkage disequilibrium (LD), haplotype analyses, and tagging SNP (tSNP) identification. Expression analyses were carried out using real-time PCR. The complete sequence of all exons and flanking intronic sequences were analyzed in DNA samples from 54 individuals affected with breast cancer from non-BRCA1/2 high-risk French Canadian breast/ovarian families. Although no germline mutation has been identified in the coding region, we identified 41 sequence variants, including 16 coding variants, 3 of which are not reported in public databases. SNP haplotypes were established and tSNPs were identified in 73 healthy unrelated French Canadians, providing a valuable tool for further association studies involving the ATR gene, using large cohorts. Our analyses led to the identification of two novel alternative splice transcripts. In contrast to the transcript generated by an alternative splicing site in the intron 41, the one resulting from a deletion of 121 nucleotides in exon 33 is widely expressed, at significant but relatively low levels, in both normal and tumoral cells including normal breast and ovarian tissue. Although no deleterious mutations were identified in the ATR gene, the current study provides an haplotype analysis of the ATR gene polymorphisms, which allowed the identification of a set of SNPs that could be used as tSNPs for large-scale association studies. In addition, our study led to the characterization of a

  19. ATR-analysaattori raaka-aineen laadunvarmistuksessa : Kasviöljyseoksen peroksidiluvun määrittäminen

    OpenAIRE

    Oinonen, Iida

    2016-01-01

    Tämän opinnäytetyön toimeksiantajana toimi Valio Oy Turengin tehtaan laadunvalvontalaboratorio. Opinnäytetyön tarkoituksena oli selvittää Thermo ScientificTM Nicolet iSTM 50 -analysaattorin ATR-mittausmoduulin käyttömahdollisuuksia raaka-aineen laadunvarmistuksessa. Tavoite oli kehittää laitteelle menetelmä, jonka avulla lastenruokien raaka-aineiden laatua voidaan tehtaalla jatkossa entistä sujuvammin valvoa. Tutkittaviksi raaka-aineiksi haluttiin lastenruoissa käytettävät jauheet, vitam...

  20. Microstructural examination of V-(3-6%)Cr-(3-5%)Ti irradiated in the ATR-A1 experiment

    Energy Technology Data Exchange (ETDEWEB)

    Gelles, D.S. [Pacific Northwest National Lab., Richland, WA (United States)

    1998-09-01

    Microstructural examination results are reported for four heats of V-(3-6%)Cr-(3-5%)Ti irradiated in the ATR-A1 experiment to {approximately}4 dpa at {approximately}200 and 300 C to provide an understanding of the microstructural evolution that may be associated with degradation of mechanical properties. Fine precipitates were observed in high density intermixed with small defect clusters for all conditions examined following the irradiation. The irradiation-induced precipitation does not appear to be affected by preirradiation heat treatment or composition.

  1. Implantes angulados en la rehabilitación parcial de sectores posteriores en maxilares atróficos

    OpenAIRE

    Casar Espinosa, Juan Carlos

    2015-01-01

    Introducción: Tras la pérdida dental se producen una serie de cambios anatómicos que dificultan rehabilitación de los sectores posteriores en maxilares atróficos. Actualmente tenemos diferentes alternativas quirúrgicas para solventar estos problemas, como pueden ser las técnicas de elevación de seno maxilar, tanto con técnicas convencionales como con el empleo de osteotomos o las técnicas M.I.A.M.B.E. Otras alternativas pueden ser el empleo de injertos óseos, las rehabilitaciones con cant...

  2. A Study of Electrochemical Reduction of Ethylene and PropyleneCarbonate Electrolytes on Graphite Using ATR-FTIR Spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Zhuang, Guorong V.; Yang, Hui; Blizanac, Berislav; Ross Jr.,Philip N.

    2005-05-12

    We present results testing the hypothesis that there is a different reaction pathway for the electrochemical reduction of PC versus EC-based electrolytes at graphite electrodes with LiPF6 as the salt in common. We examined the reduction products formed using ex-situ Fourier Transform Infrared (FTIR) spectroscopy in attenuated total reflection (ATR) geometry. The results show the pathway for reduction of PC leads nearly entirely to lithium carbonate as the solid product (and presumably ethylene gas as the co-product) while EC follows a path producing a mixture of organic and inorganic compounds. Possible explanations for the difference in reaction pathway are discussed.

  3. Tip-enhanced near-field optical microscope with side-on and ATR-mode sample excitation for super-resolution Raman imaging of surfaces

    Science.gov (United States)

    Heilman, A. L.; Gordon, M. J.

    2016-06-01

    A tip-enhanced near-field optical microscope with side-on and attenuated total reflectance (ATR) excitation and collection is described and used to demonstrate sub-diffraction-limited (super-resolution) optical and chemical characterization of surfaces. ATR illumination is combined with an Au optical antenna tip to show that (i) the tip can quantitatively transduce the optical near-field (evanescent waves) above the surface by scattering photons into the far-field, (ii) the ATR geometry enables excitation and characterization of surface plasmon polaritons (SPPs), whose associated optical fields are shown to enhance Raman scattering from a thin layer of copper phthalocyanine (CuPc), and (iii) SPPs can be used to plasmonically excite the tip for super-resolution chemical imaging of patterned CuPc via tip-enhanced Raman spectroscopy (TERS). ATR-illumination TERS is also quantitatively compared with the more conventional side-on illumination scheme. In both cases, spatial resolution was better than 40 nm and tip on/tip off Raman enhancement factors were >6500. Furthermore, ATR illumination was shown to provide similar Raman signal levels at lower "effective" pump powers due to additional optical energy delivered by SPPs to the active region in the tip-surface gap.

  4. Direct Binding to Replication Protein A (RPA)-coated Single-stranded DNA Allows Recruitment of the ATR Activator TopBP1 to Sites of DNA Damage.

    Science.gov (United States)

    Acevedo, Julyana; Yan, Shan; Michael, W Matthew

    2016-06-17

    A critical event for the ability of cells to tolerate DNA damage and replication stress is activation of the ATR kinase. ATR activation is dependent on the BRCT (BRCA1 C terminus) repeat-containing protein TopBP1. Previous work has shown that recruitment of TopBP1 to sites of DNA damage and stalled replication forks is necessary for downstream events in ATR activation; however, the mechanism for this recruitment was not known. Here, we use protein binding assays and functional studies in Xenopus egg extracts to show that TopBP1 makes a direct interaction, via its BRCT2 domain, with RPA-coated single-stranded DNA. We identify a point mutant that abrogates this interaction and show that this mutant fails to accumulate at sites of DNA damage and that the mutant cannot activate ATR. These data thus supply a mechanism for how the critical ATR activator, TopBP1, senses DNA damage and stalled replication forks to initiate assembly of checkpoint signaling complexes.

  5. [A case of X-linked alpha-thalassemia/mental retardation (ATR-X) syndrome with repeated apnea attacks due to laryngomalacia].

    Science.gov (United States)

    Ebishima, Yuko; Misaki, Takako; Owa, Kenji; Okuno, Takehiko; Wada, Takahito; Suehiro, Yutaka

    2013-01-01

    We report a case of X-linked alpha-thalassemia/mental retardation syndrome (ATR-X) with repeated apnea attacks dating from the patient's 12th year. We initially diagnosed them as obstructive apnea due to upper pharyngeal stenosis and laryngomalacia by polysomnography and laryngo-fiberscopy. However, reevaluation after one and a half years revealed that the boy had central and mixed apnea, as well as obstructive apnea. To date, few reports have been published on the causes of apnea attacks in ATR-X patients. We clinicians should therefore consider laryngomalacia as one cause of apnea attacks in ATR-X patients, and choose the appropriate therapy for a pattern of apnea that can change during its clinical course. PMID:23593745

  6. ATR FT-IR spectroscopy on Vmh2 hydrophobin self-assembled layers for Teflon membrane bio-functionalization

    International Nuclear Information System (INIS)

    Graphical abstract: - Highlights: • Hydrophobin self-assembled layers on Teflon in different preparation conditions were investigated. • ATR collection data geometry allowed samples examination without any particular preparation. • Amide content, lipid/amide and carbohydrate/amide ratios of the protein layer were estimated. • Secondary structure of protein was determined for the examined samples. • FT-IR demonstrated to be of extreme relevance in monitoring hydrophobin self-assembled layers preparation. - Abstract: Surface functionalization by layers of hydrophobins, amphiphilic proteins produced by fungi offers a promising and green strategy for fabrication of biomedical and bioanalytical devices. The layering process of the Vmh2 hydrophobin from Pleurotus ostreatus on Teflon membrane has been investigated by Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) spectroscopy. In particular, protein layers obtained with hydrophobin purified with two different procedures and in various coating conditions have been examined. The layers have been characterized by quantifying the amide I and amide II band area together with the lipid/amide ratio and carbohydrate/amide ratio. This characterization can be very useful in evaluating the best purification strategy and coating conditions. Moreover the analysis of the secondary structure of the layered protein using the deconvolution procedure of amide I band indicate the prevalent contribution from β-sheet state. The results inferred by infrared spectroscopy have been also confirmed by scanning electron microscopy imaging

  7. ATR FT-IR spectroscopy on Vmh2 hydrophobin self-assembled layers for Teflon membrane bio-functionalization

    Energy Technology Data Exchange (ETDEWEB)

    Portaccio, M., E-mail: marianna.portaccio@unina2.it [Dipartimento di Medicina Sperimentale – Seconda Università di Napoli, Via S.M. di Costantinopoli, 16-80134 Napoli (Italy); Gravagnuolo, A.M., E-mail: alfredomaria.gravagnuolo@unina.it [Dipartimento di Scienze Chimiche, Università “Federico II”, Via Cintia, 21- 80126 Napoli (Italy); Longobardi, S., E-mail: sara.longobardi@unina.it [Dipartimento di Scienze Chimiche, Università “Federico II”, Via Cintia, 21- 80126 Napoli (Italy); Giardina, P., E-mail: paola.giardina@unina.it [Dipartimento di Scienze Chimiche, Università “Federico II”, Via Cintia, 21- 80126 Napoli (Italy); Rea, I., E-mail: ilaria.rea@na.imm.cnr.it [Institute for Microelectronics and Microsystems, CNR, Via P. Castellino, 111-80131 Napoli (Italy); De Stefano, L., E-mail: luca.destefano@na.imm.cnr.it [Institute for Microelectronics and Microsystems, CNR, Via P. Castellino, 111-80131 Napoli (Italy); Cammarota, M., E-mail: marcella.cammarota@unina2.it [Dipartimento di Medicina Sperimentale – Seconda Università di Napoli, Via S.M. di Costantinopoli, 16-80134 Napoli (Italy); Lepore, M., E-mail: maria.lepore@unina2.it [Dipartimento di Medicina Sperimentale – Seconda Università di Napoli, Via S.M. di Costantinopoli, 16-80134 Napoli (Italy)

    2015-10-01

    Graphical abstract: - Highlights: • Hydrophobin self-assembled layers on Teflon in different preparation conditions were investigated. • ATR collection data geometry allowed samples examination without any particular preparation. • Amide content, lipid/amide and carbohydrate/amide ratios of the protein layer were estimated. • Secondary structure of protein was determined for the examined samples. • FT-IR demonstrated to be of extreme relevance in monitoring hydrophobin self-assembled layers preparation. - Abstract: Surface functionalization by layers of hydrophobins, amphiphilic proteins produced by fungi offers a promising and green strategy for fabrication of biomedical and bioanalytical devices. The layering process of the Vmh2 hydrophobin from Pleurotus ostreatus on Teflon membrane has been investigated by Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) spectroscopy. In particular, protein layers obtained with hydrophobin purified with two different procedures and in various coating conditions have been examined. The layers have been characterized by quantifying the amide I and amide II band area together with the lipid/amide ratio and carbohydrate/amide ratio. This characterization can be very useful in evaluating the best purification strategy and coating conditions. Moreover the analysis of the secondary structure of the layered protein using the deconvolution procedure of amide I band indicate the prevalent contribution from β-sheet state. The results inferred by infrared spectroscopy have been also confirmed by scanning electron microscopy imaging.

  8. Department of Energy's Advanced Test Reactor (ATR), July 14--18, 1980: An independent on-site safety review

    International Nuclear Information System (INIS)

    The intent of this review was not to conduct a detailed in-depth audit, but rather to make a broad management assessment of ATR operations. The results of the review should only be considered as having identified trends or indications. The Team's observations and recommendations for the most part are based upon standards used for licensed reactor facility practices. These standards form the basis for many of the comments in this report. The Team believes that a uniform minimum standard of performance should be achieved in the operation of DOE reactors. In order to assure that this is accomplished, clear standards are necessary. Consistent with the past AEC and ERDA policy, the Team has used the standards of the commercial nuclear power industry. It is recognized that this approach is conservative, in that the ATR reactor has a significantly greater degree of inherent safety (lower pressure, temperature, power, etc.) than a licensed reactor. Although the Review Team found no indications or evidence that the plant is being operated in an unsafe manner, various areas were identified where improvements are either needed or should be considered to increase the safety of reactor operations

  9. SAXS and ATR-FTIR studies on EBT-TSX mixtures in their sol-gel phases.

    Science.gov (United States)

    Hirun, Namon; Rugmai, Supagorn; Sangfai, Tanatchaporn; Tantishaiyakul, Vimon

    2012-11-01

    Our previous study demonstrated that mixtures of tamarind seed xyloglucan (TSX) with appropriate concentrations of eriochrome black T (EBT) produced a gel that could be of benefit for medical use. Here, the sol-gel systems of various fresh and aged mixtures were further investigated using rheological measurements. The nanostructural changes of EBT-TSX sol-gel phases were analyzed using SAXS. The interactions between EBT and TSX in the sol and gel states were examined using ATR-FTIR. SAXS data analysis demonstrated that the mixture containing lower concentration of EBT formed rod-like structures and that with higher concentrations of EBT produced flat particles. The sizes of the TSX structures from the aged mixtures in the gel stage were larger than those from the same mixtures in the sol state. ATR-FTIR spectral changes revealed that the azo and sulfonic acid groups of EBT interacted with the TSX, and the characteristic spectrum of the sulfonic acid group of EBT could discriminate between the sol and gel state of the EBT-TSX systems. The interactions between EBT and TSX may cause conformational changes to TSX and facilitate the sol-gel transition or formation of a gel.

  10. ATR-IR fingerprinting as a powerful method for identification of traditional medicine samples: a report of 20 herbal patterns

    Directory of Open Access Journals (Sweden)

    A. Sakhteman

    2015-06-01

    Full Text Available Background and objectives: Attenuated total reflectance-inferared (ATR-IR spectra can be used as a non-invasive fingerprinting approach in quality control of herbal samples. Methods: Twenty versatile herbal samples were subjected to attenuated total reflectance-inferared (ATR-IR spectroscopy followed by different clustering methods in order to determine by which method more reasonable classifications would be obtained. Results: All classification methods (K-means, HCA, PCA and SOM were able to discriminate the two medicinal seeds, Hyocyamus niger and Peganum harmala from other herbal samples. Similarly, the starch samples were clustered in a reasonable method. In HCA, one cluster included three types of starch samples: Zea mays, Oryza sativa and Triticum aestivum. All the four classification methods were able to separate Solanum tuberosum starch from other starch samples. HCA and SOM, were able to classify leaf samples Origanum vulgare and Melissa officinalis belonging to Lamiaceae family, in one category. Crocus sativus and its adulterant Carthamus tinctorius flowers were identified by PCA, HCA and SOM as different categories. Conclusion: The result of this study can be utilized for identification and quality control of traditionally used medicinal plant samples in an unknown sample powder. Such data could be the basis for preparing a data bank on Iranian medicinal samples which in turn is used as a simple, fast and reliable method for characterization of herbal powders in Pharmacopoeias.

  11. In Situ ATR FTIR Spectroscopic Study of the Formation and Hydration of a Fucoidan/Chitosan Polyelectrolyte Multilayer.

    Science.gov (United States)

    Ho, Tracey T M; Bremmell, Kristen E; Krasowska, Marta; MacWilliams, Stephanie V; Richard, Céline J E; Stringer, Damien N; Beattie, David A

    2015-10-20

    The formation of fucoidan/chitosan-based polyelectrolyte multilayers (PEMs) has been studied with in situ Fourier transform infrared (FTIR) spectroscopy. Attenuated total reflectance (ATR) FTIR spectroscopy has been used to follow the sequential build-up of the multilayer, with peaks characteristic of each polymer being seen to increase in intensity with each respective adsorption stage. In addition, spectral processing has allowed for the extraction of spectra from individual adsorbed layers, which have been used to provide unambiguous determination of the adsorbed mass of the PEM at each stage of formation. The PEM was seen to undergo a transition in growth regimes during build-up: from supra-linear to linear. In addition, the wettability of the PEM has been probed at each stage of the build-up, using the captive bubble contact angle technique. The contact angles were uniformly low, but showed variation in value depending on the nature of the outer polymer layer, and this variation correlated with the overall percentage hydration of the PEM (determined from FTIR and quartz crystal microbalance data). The nature of the hydration water within the polyelectrolyte multilayer has also been studied with FTIR spectroscopy, specifically in situ synchrotron ATR FTIR microscopy of the multilayer confined between two solid surfaces. The acquired spectra have enabled the hydrogen bonding environment of the PEM hydration water to be determined. The PEM hydration water is seen to have an environment in which it is subject to fewer hydrogen bonding interactions than in bulk electrolyte solution. PMID:26421938

  12. In-situ monitoring of blood glucose level for dialysis machine by AAA-battery-size ATR Fourier spectroscopy

    Science.gov (United States)

    Hosono, Satsuki; Sato, Shun; Ishida, Akane; Suzuki, Yo; Inohara, Daichi; Nogo, Kosuke; Abeygunawardhana, Pradeep K.; Suzuki, Satoru; Nishiyama, Akira; Wada, Kenji; Ishimaru, Ichiro

    2015-07-01

    For blood glucose level measurement of dialysis machines, we proposed AAA-battery-size ATR (Attenuated total reflection) Fourier spectroscopy in middle infrared light region. The proposed one-shot Fourier spectroscopic imaging is a near-common path and spatial phase-shift interferometer with high time resolution. Because numerous number of spectral data that is 60 (= camera frame rare e.g. 60[Hz]) multiplied by pixel number could be obtained in 1[sec.], statistical-averaging improvement realize high-accurate spectral measurement. We evaluated the quantitative accuracy of our proposed method for measuring glucose concentration in near-infrared light region with liquid cells. We confirmed that absorbance at 1600[nm] had high correlations with glucose concentrations (correlation coefficient: 0.92). But to measure whole-blood, complex light phenomenon caused from red blood cells, that is scattering and multiple reflection or so, deteriorate spectral data. Thus, we also proposed the ultrasound-assisted spectroscopic imaging that traps particles at standing-wave node. Thus, if ATR prism is oscillated mechanically, anti-node area is generated around evanescent light field on prism surface. By elimination complex light phenomenon of red blood cells, glucose concentration in whole-blood will be quantify with high accuracy. In this report, we successfully trapped red blood cells in normal saline solution with ultrasonic standing wave (frequency: 2[MHz]).

  13. ATR-FTIR spectroscopic studies on density changes of fused silica induced by localized CO2 laser treatment

    Institute of Scientific and Technical Information of China (English)

    张传超; 郑万国; 袁晓东; 张丽娟; 廖威; 晏中华; 陈静; 蒋一岚; 王海军; 栾晓雨; 叶亚云

    2015-01-01

    Surface density changes of the central region of the sites treated by using the CO2 laser-based non-evaporative dam-age mitigation for fused silica are investigated by the attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). The ATR-FTIR peak shifts of the treated sites of fused silica are monitored to determine the changes of the corresponding density. For the quenching treated sites, the surface density is increased by (0.24±0.01)%compared with the initial density, but the laser annealing by the exposure of a power ramp down after damage mitigation effectively suppresses the structural changes of treated sites, which could reduce the increase of the corresponding density to (0.08±0.01)%. The results provide the sufficient evidence that the laser annealing by a power ramp down after damage mitigation has a positive effect on the control of the structural change induced by CO2 laser-based damage mitigation.

  14. Galiellalactone induces cell cycle arrest and apoptosis through the ATM/ATR pathway in prostate cancer cells.

    Science.gov (United States)

    García, Víctor; Lara-Chica, Maribel; Cantarero, Irene; Sterner, Olov; Calzado, Marco A; Muñoz, Eduardo

    2016-01-26

    Galiellalactone (GL) is a fungal metabolite that presents antitumor activities on prostate cancer in vitro and in vivo. In this study we show that GL induced cell cycle arrest in G2/M phase, caspase-dependent apoptosis and also affected the microtubule organization and migration ability in DU145 cells. GL did not induce double strand DNA break but activated the ATR and ATM-mediated DNA damage response (DDR) inducing CHK1, H2AX phosphorylation (fH2AX) and CDC25C downregulation. Inhibition of the ATM/ATR activation with caffeine reverted GL-induced G2/M cell cycle arrest, apoptosis and DNA damage measured by fH2AX. In contrast, UCN-01, a CHK1 inhibitor, prevented GL-induced cell cycle arrest but enhanced apoptosis in DU145 cells. Furthermore, we found that GL did not increase the levels of intracellular ROS, but the antioxidant N-acetylcysteine (NAC) completely prevented the effects of GL on fH2AX, G2/M cell cycle arrest and apoptosis. In contrast to NAC, other antioxidants such as ambroxol and EGCG did not interfere with the activity of GL on cell cycle. GL significantly suppressed DU145 xenograft growth in vivo and induced the expression of fH2AX in the tumors. These findings identify for the first time that GL activates DDR in prostate cancer.

  15. Review of reactor physics activities relevant to FBR and ATR programmes in PNC, Japan, June 1977 to October 1978

    International Nuclear Information System (INIS)

    Reactor physics works in Power Reactor and Nuclear Fuel Development Corporation (PNC) are carried out in support of the FBR and ATR development programmes. The works are in progress with high efficiency, in cooperation of other related organizations in Japan and also overseas. The experimental fast reactor ''Joyo'' reached its full power of 50 MWt, being operated now to obtain technical data and experiences. The prototype fast breeder reactor ''Monju'' is nearing completion of its final design, and the surveys on its proposed site have been finished. The advanced thermal reactor ''Fugen'', a heavy water-moderated and boiling light water-cooled, reactor reached the criticality, and the power generation was successfully achieved. The data to be obtained are used for the development of a large demonstration reactor. After describing on these types of reactors briefly, the works on reactor physics are described as follows: for fast reactors, mockup experiment and analysis, evaluation of actinide nuclear data, development of core analytical method, and research on shielding; and for ATR, research with a deuterium critical assembly. (Mori, K.)

  16. Quickly Identifying Different Species of Lavender Essential Oils by ATR-FTIR Coupled to PCA and DPLS%ATR-FTIR结合PCA及DPLS快速识别不同品种薰衣草精油

    Institute of Scientific and Technical Information of China (English)

    童红; 唐军; 张正方

    2014-01-01

    筛选有代表性的4个不同品种的96个薰衣草精油样品为试验对象,应用衰减全反射傅里叶变换红外光谱(ATR-FTIR)获得光谱数据,在主成分分析的基础上采用偏最小二乘辨别法(DPLS)建立薰衣草精油品种识别的模型。结果表明:主成分分析基本能实现品种区分,DPLS 法以68个样本作为校正集,交叉验证均方根标准差(RMSEE)为0.1590,以未知的28个样本进行预测,预测均方根标准差(RMSEP)为0.1860,品种识别率为100%,DPLS 法建立的模型对薰衣草精油品种有很好的识别和分类能力。将红外光谱与化学计量学方法结合,可为薰衣草精油品种的优良宏观质控提供一个快速、直观、可行的方法。%Use Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) to obtain spectral data for classification according to lavender oil cultivars,as a tool to guarantee varie-tal authenticity.96 samples belong to four most representative lavender cultivars.On the basis of PCA,its performance is also compared with that obtained using DPLS model.The results show that PCA can basically discriminate lavender oil cultivar.68 samples are as a calibration set in DPLS method,the RMSEE is 0.1590,28 unknown samples are as predictive samples,the RMSEP is 0. 1860;the correct identification rate of species is 100%.Based on these two data sets only,it seems that the DPLS method is the best of those considered here.The result indicates that a model which provides a quick,intuitive and feasible method has been built to discriminate lavender oils by using ATR-FTIR method combined with chemometrics techniques.

  17. Revisiting the Brønsted acid catalysed hydrolysis kinetics of polymeric carbohydrates in ionic liquids by in situ ATR-FTIR spectroscopy

    DEFF Research Database (Denmark)

    Kunov-Kruse, Andreas Jonas; Riisager, Anders; Shunmugavel, Saravanamurugan;

    2013-01-01

    A new versatile method to measure rates and determine activation energies for the Brønsted acid catalysed hydrolysis of cellulose and cellobiose (and other polymeric carbohydrates) in ionic liquids is demonstrated by following the C–O stretching band of the glycoside bond with in situ ATR-FTIR. A...

  18. Formation of carboxy- and amide-terminated alkyl monolayers on silicon(111) investigated by ATR-FTIR, XPS, and X-ray scattering: Construction of photoswitchable surfaces

    DEFF Research Database (Denmark)

    Rück-Braun, Karola; Petersen, Michael Åxman; Michalik, Fabian;

    2013-01-01

    -FTIR and XPS studies of the fulgimide samples revealed closely covered amide-terminated SAMs. Reversible photoswitching of the headgroup was read out by applying XPS, ATR-FTIR, and difference absorption spectra in the mid-IR. In XPS, we observed a reversible breathing of the amide/imide C1s and N1s signals...

  19. Measuring Heterogeneous Reaction Rates with ATR-FTIR Spectroscopy to Evaluate Chemical Fates in an Atmospheric Environment: A Physical Chemistry and Environmental Chemistry Laboratory Experiment

    Science.gov (United States)

    Roberts, Jason E.; Zeng, Guang; Maron, Marta K.; Mach, Mindy; Dwebi, Iman; Liu, Yong

    2016-01-01

    This paper reports an undergraduate laboratory experiment to measure heterogeneous liquid/gas reaction kinetics (ozone-oleic acid and ozone-phenothrin) using a flow reactor coupled to an attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectrometer. The experiment is specially designed for an upper-level undergraduate Physical…

  20. Fabrication Control Plan for ORNL RH-LOCA ATF Test Specimens to be Irradiated in the ATR

    Energy Technology Data Exchange (ETDEWEB)

    Kevin G. Field; Richard Howard; Michael Teague

    2014-06-01

    The purpose of this fabrication plan is (1) to summarize the design of a set of rodlets that will be fabricated and then irradiated in the Advanced Test Reactor (ATR) and (2) provide requirements for fabrication and acceptance criteria for inspections of the Light Water Reactor (LWR) – Accident Tolerant Fuels (ATF) rodlet components. The functional and operational (F&OR) requirements for the ATF program are identified in the ATF Test Plan. The scope of this document only covers fabrication and inspections of rodlet components detailed in drawings 604496 and 604497. It does not cover the assembly of these items to form a completed test irradiation assembly or the inspection of the final assembly, which will be included in a separate INL final test assembly specification/inspection document. The controls support the requirements that the test irradiations must be performed safely and that subsequent examinations must provide valid results.

  1. Inhibition of human Chk1 causes increased initiation of DNA replication, phosphorylation of ATR targets, and DNA breakage

    DEFF Research Database (Denmark)

    Syljuåsen, Randi G; Sørensen, Claus Storgaard; Hansen, Lasse Tengbjerg;

    2005-01-01

    -nuclear phosphorylation of histone H2AX, p53, Smc1, replication protein A, and Chk1 itself in human S-phase cells. These phosphorylations were inhibited by ATR siRNA and caffeine, but they occurred independently of ATM. Chk1 inhibition also caused an increased initiation of DNA replication, which was accompanied by...... increased amounts of nonextractable RPA protein, formation of single-stranded DNA, and induction of DNA strand breaks. Moreover, these responses were prevented by siRNA-mediated downregulation of Cdk2 or the replication initiation protein Cdc45, or by addition of the CDK inhibitor roscovitine. We propose...... that Chk1 is required during normal S phase to avoid aberrantly increased initiation of DNA replication, thereby protecting against DNA breakage. These results may help explain why Chk1 is an essential kinase and should be taken into account when drugs to inhibit this kinase are considered for use in...

  2. Rapid evaluation and quantitative analysis of thyme, origano and chamomile essential oils by ATR-IR and NIR spectroscopy

    Science.gov (United States)

    Schulz, Hartwig; Quilitzsch, Rolf; Krüger, Hans

    2003-12-01

    The essential oils obtained from various chemotypes of thyme, origano and chamomile species were studied by ATR/FT-IR as well as NIR spectroscopy. Application of multivariate statistics (PCA, PLS) in conjunction with analytical reference data leads to very good IR and NIR calibration results. For the main essential oil components (e.g. carvacrol, thymol, γ-terpinene, α-bisabolol and β-farnesene) standard errors are in the range of the applied GC reference method. In most cases the multiple coefficients of determination ( R2) are >0.97. Using the IR fingerprint region (900-1400 cm -1) a qualitative discrimination of the individual chemotypes is possible already by visual judgement without to apply any chemometric algorithms.The described rapid and non-destructive methods can be applied in industry to control very easily purifying, blending and redistillation processes of the mentioned essential oils.

  3. A hybrid original approach for prediction of the aerodynamic coefficients of an ATR-42 scaled wing model

    Directory of Open Access Journals (Sweden)

    Abdallah Ben Mosbah

    2016-02-01

    Full Text Available A new approach for the prediction of lift, drag, and moment coefficients is presented. This approach is based on the support vector machines (SVMs methodology and an optimization meta-heuristic algorithm called extended great deluge (EGD. The novelty of this approach is the hybridization between the SVM and the EGD algorithm. The EGD is used to optimize the SVM parameters. The training and validation of this new identification approach is realized using the aerodynamic coefficients of an ATR-42 wing model. The aerodynamic coefficients data are obtained with the XFoil software and experimental tests using the Price–Païdoussis wind tunnel. The predicted results with our approach are compared with those from the XFoil software and experimental results for different flight cases of angles of attack and Mach numbers. The main purpose of this methodology is to rapidly predict aircraft aerodynamic coefficients.

  4. ATR-IR spectroscopic study of the structural changes in the hydrophobic region of ICPAN/DPPC bilayers

    Science.gov (United States)

    Cieślik-Boczula, Katarzyna; Czarnik-Matusewicz, Bogusława; Perevozkina, Margarita; Filarowski, Aleksander; Boens, Noël; De Borggraeve, Wim M.; Koll, Aleksander

    2008-04-01

    Structural changes in the hydrophobic region in the self-aggregates of the long hydrocarbon chain ICPAN ((( N, N-dimethyl- N-octyl-2-ammonioethyl)-3-(3,5-di- tert-butyl-4-hydroxyphenyl)propionate bromide)) homologues and their interaction with a DPPC (dipalmitoylophosphatidylcholine) bilayer were the subject of detailed investigation using ATR infrared spectroscopy. On the base of analysis of the bands assigned to CH 2 stretching, scissoring, and rocking and CH wagging vibrations it was revealed that the hydrophobic parts ICPANs adopt a gauche-rich disordering structure. Moreover, it was shown that the micellar- or lamellar-like character of these structures depends on the hydrocarbon chain length. Results obtained from the ICPAN/DPPC mixtures indicate an increase in the conformational disorder in hydrophobic part compare to pure DPPC film. This effect depends on the length of the aliphatic chain of ICPAN homologues and on the relative concentrations of DPPC and ICPAN.

  5. Formulation and drug-content assay of microencapsulated antisense oligonucleotide to NF-κB using ATR-FTIR

    International Nuclear Information System (INIS)

    Antisense oligonucleotide to NF-κB sequence: 5′-GGA AAC ACA TCC TCC ATG-3′, was microencapsulated in an albumin matrix by the method of spray dryingTM. Spectral analysis was performed on varying drug loading formulations of both drugs by mid-IR attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). An out of plane O–H bending vibration at 948 cm−1, unique to both the native and microencapsulated drugs was identified. The calculated peak areas corresponded to the drug loadings in the microsphere formulations. A standard curve could then be used to determine the drug content of an unknown microsphere formulation. Accuracy and precision were determined to be comparable to other analytical techniques such as HPLC. (paper)

  6. A Simple Approach to Distinguish Classic and Formaldehyde-Free Tannin Based Rigid Foams by ATR FT-IR

    Directory of Open Access Journals (Sweden)

    Gianluca Tondi

    2015-01-01

    Full Text Available Tannin based rigid foams (TBRFs have been produced with formaldehyde since 1994. Only recently several methods have been developed in order to produce these foams without using formaldehyde. TBRFs with and without formaldehyde are visually indistinguishable; therefore a method for determining the differences between these foams had to be found. The attenuated total reflectance infrared spectroscopy (ATR FT-IR investigation of the TBRFs presented in this paper allowed discrimination between the formaldehyde-containing (classic and formaldehyde-free TBRFs. The spectra of the formaldehyde-free TBRFs, indeed, present decreased band intensity related to the C–O stretching vibration of (i the methylol groups and (ii the furanic rings. This evidence served to prove the chemical difference between the two TBRFs and explained the slightly higher mechanical properties measured for the classic TBRFs.

  7. A distrofia miotônica ou miotonia atrófica: estudo sintético-crítico

    Directory of Open Access Journals (Sweden)

    Enzo Azzi

    1953-03-01

    Full Text Available O autor, baseado em algumas observações clínicas pessoais, individualiza os elementos principais (atróficos, miotônicos, neurendócrino-vegetativos que se referem à distrofia miotônica, insistindo sôbre a riqueza dos sintomas infundíbulo-hipofisários observados (distrofia adiposogenital, policitemia, perturbações do sono, constante hipoplasia da sela turca que falam em favor de uma meiopragia diencéfalo-hipofisária como mecanismo fisiopatogênico da doença. O autor discute também o problema das relações entre distrofia miotônica e miotonia de Thomsen, concluindo, com base em um de seus casos, pela identidade fundamental das duas formas e, portanto, pela sua unificação.

  8. The Conference Proceedings of the 2001 Air Transport Research Society (ATRS) of the WCTR Society. Volume 1

    Science.gov (United States)

    Lee, Yeong-Heok (Editor); Bowen, Brent D. (Editor); Tarry, Scott E. (Editor)

    2001-01-01

    The ATRS held its Annual conference at Jeju Island, Korea in July 2001. The conference was a success with nearly 140 participants including 70 presenters. This report contains presentations from Volume 1 on the following: Airline and Travel Agent Relationships in Asia;Benchmarking Aviation Safety in the Commercial Airline Industry;Impact of Frequent Flyer Program on the Demand for Air Travel; Application of Genetic Algorithm on Airline Schedule;The Effects of Dual Carrier Designation and Partial Liberalization: The Case of Canada;Defense of Air Carriers and Air Agencies in FAA Enforcement proceedin gs - Damage Control Before the Case Arises; Cost Incentives for Airline Mergers? - An examination on the cost impact of U.S. airline mergers and acquisitions;Airport Regulation, Airline Competition and Canada's Airport System; Airline Competition: The Case of Israel's Domestic Doupoly; Non-Financial Indicators of Airline Distress: A Conceptual Approach;and Airport Privatization: An Empirical Analysis of Financial and Operational Efficiency.

  9. A hybrid original approach for prediction of the aerodynamic coefficients of an ATR-42 scaled wing model

    Institute of Scientific and Technical Information of China (English)

    Abdallah; en Mosbah; Ruxandra Mihaela; otez; Thien My; ao

    2016-01-01

    A new approach for the prediction of lift, drag, and moment coefficients is presented. This approach is based on the support vector machines (SVMs) methodology and an optimization meta-heuristic algorithm called extended great deluge (EGD). The novelty of this approach is the hybridization between the SVM and the EGD algorithm. The EGD is used to optimize the SVM parameters. The training and validation of this new identification approach is realized using the aerodynamic coefficients of an ATR-42 wing model. The aerodynamic coefficients data are obtained with the XFoil software and experimental tests using the Price–Paıdoussis wind tunnel. The predicted results with our approach are compared with those from the XFoil software and experimental results for different flight cases of angles of attack and Mach numbers. The main pur-pose of this methodology is to rapidly predict aircraft aerodynamic coefficients.

  10. Tensile and impact properties of vanadium-base alloys irradiated at low temperatures in the ATR-A1 experiment

    Energy Technology Data Exchange (ETDEWEB)

    Tsai, H.; Nowicki, L.J.; Billone, M.C.; Chung, H.M.; Smith, D.L. [Argonne National Lab., IL (United States)

    1998-03-01

    Subsize tensile and Charpy specimens made from several V-(4-5)Cr-(4-5)Ti alloys were irradiated in the ATR-A1 experiment to study the effects of low-temperature irradiation on mechanical properties. These specimens were contained in lithium-bonded subcapsules and irradiated at temperatures between {approx}200 and 300 C. Peak neutron damage was {approx}4.7 dpa. Postirradiation testing of these specimens has begun. Preliminary results from a limited number of specimens indicate a significant loss of work-hardening capability and dynamic toughness due to the irradiation. These results are consistent with data from previous low-temperature neutron irradiation experiments on these alloys.

  11. Formulation and drug-content assay of microencapsulated antisense oligonucleotide to NF-κB using ATR-FTIR

    Science.gov (United States)

    Siwale, Rodney; Meadows, Fred; Mody, Vicky V.; Shah, Samit

    2013-09-01

    Antisense oligonucleotide to NF-κB sequence: 5‧-GGA AAC ACA TCC TCC ATG-3‧, was microencapsulated in an albumin matrix by the method of spray dryingTM. Spectral analysis was performed on varying drug loading formulations of both drugs by mid-IR attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). An out of plane O-H bending vibration at 948 cm-1, unique to both the native and microencapsulated drugs was identified. The calculated peak areas corresponded to the drug loadings in the microsphere formulations. A standard curve could then be used to determine the drug content of an unknown microsphere formulation. Accuracy and precision were determined to be comparable to other analytical techniques such as HPLC.

  12. Cellulose acetate-lithium bis(trifluoromethanesulfonyl)imide solid polymer electrolyte: ATR-FTIR and ionic conductivity behavior

    Science.gov (United States)

    Mohd Razalli, Siti Masyitah; Sheikh Mohd Saaid, Siti Irma Yuana; Marwan Ali, Ab Malik; Hassan, Oskar Hasdinor; Yahya, Muhd Zu Azhan

    2015-05-01

    Solid polymer electrolytes (SPEs) based on cellulose acetate (CA) doped with lithium bis(trifluoromethanesulfonyl)imide (LiTFSI) salt are prepared by solution cast technique. Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy of the polymer salt complexes are recorded in the frequency range between 400 cm-1 and 4000 cm-1. The shifting of carbonyl band (C=O) at 1737 cm-1 to a lower wavenumber confirms the occurrence of complexation between the polymer and the salt. The electrochemical impedance spectroscopy (EIS) analysis discovered that the film with 25 wt.% of salt shows the highest ionic conductivity at room temperature. The change in real dielectric permittivity (ɛr) as a function of frequency at different salt concentrations which exhibits a dispersive behavior at low frequencies and decays at higher frequencies, shows the electrode polarization and space charge effect. The real modulus formalism (Mr) analysis shows that the polymer electrolytes in this work are ionic conductors.

  13. Overview of aerosol properties associated with air masses sampled by the ATR-42 during the EUCAARI campaign (2008)

    Science.gov (United States)

    Crumeyrolle, S.; Schwarzenboeck, A.; Roger, J. C.; Sellegri, K.; Burkhart, J. F.; Stohl, A.; Gomes, L.; Quennehen, B.; Roberts, G.; Weigel, R.; Villani, P.; Pichon, J. M.; Bourrianne, T.; Laj, P.

    2013-05-01

    Within the frame of the European Aerosol Cloud Climate and Air Quality Interactions (EUCAARI) project, the Météo-France aircraft ATR-42 performed 22 research flights over central Europe and the North Sea during the intensive observation period in May 2008. For the campaign, the ATR-42 was equipped to study the aerosol physical, chemical, hygroscopic and optical properties, as well as cloud microphysics. For the 22 research flights, retroplume analyses along the flight tracks were performed with FLEXPART in order to classify air masses into five sectors of origin, allowing for a qualitative evaluation of emission influence on the respective air parcel. This study shows that the extensive aerosol parameters (aerosol mass and number concentrations) show vertical decreasing gradients and in some air masses maximum mass concentrations (mainly organics) in an intermediate layer (1-3 km). The observed mass concentrations (in the boundary layer (BL): between 10 and 30 μg m-3; lower free troposphere (LFT): 0.8 and 14 μg m-3) are high especially in comparison with the 2015 European norms for PM2.5 (25 μg m-3) and with previous airborne studies performed over England (Morgan et al., 2009; McMeeking et al., 2012). Particle number size distributions show a larger fraction of particles in the accumulation size range in the LFT compared to BL. The chemical composition of submicron aerosol particles is dominated by organics in the BL, while ammonium sulphate dominates the submicron aerosols in the LFT, especially in the aerosol particles originated from north-eastern Europe (~ 80%), also experiencing nucleation events along the transport. As a consequence, first the particle CCN acting ability, shown by the CCN/CN ratio, and second the average values of the scattering cross sections of optically active particles (i.e. scattering coefficient divided by the optical active particle concentration) are increased in the LFT compared to BL.

  14. Low-temperature, low-cost growth of robust ATR GeO2 hollow fibers based on copper capillary tubes for transmission of CO2 laser light

    Science.gov (United States)

    Jing, Chengbin; Bai, Wei; Hu, Zhigao; Yang, Pingxiong; Liu, Aiyun; Lin, Fangtin; Shi, Yiwei; Chu, Junhao

    2014-02-01

    Attenuated total reflectance (ATR) infrared hollow waveguide attracts particular interest since it has both advantages of a hollow fiber and a light guiding mechanism similar to that of solid-core fibers. Presently, ATR hollow waveguides are mainly structured with single-crystal sapphire or glassy materials. These waveguides are somewhat brittle. More robust ATR hollow fibers are required in many military and domestic applications. In this work, ATR GeO2 hollow waveguides were prepared based on a copper capillary tube for transmitting CO2 laser light. The inner wall of the copper structural tube was polished using a high-pressure pulsed nanofluid technique. A hexagonal crystalline GeO2 reflective layer with sufficient thickness (>4 μm) was grown on the inner tube wall via a simple liquid phase deposition process at room temperature. The GeO2 coated copper hollow fiber exhibits a low-loss band within 10-11.5 μm. It can still be bent since the hollow-core size (1.4 mm) and the wall thickness (50 μm) are not too large. The transmissions of CO2 laser light are 91% and 43% under a straight condition and a 90° bend with a 30-cm radius condition, respectively. The waveguide displays high heat-resisting properties due to high thermal conductivity of the copper substrate tube and a high melting point (1115°C) of the GeO2 reflective layer. This work opens a door for low-temperature, low-cost growth of long ATR GeO2 infrared hollow fibers based on various substrate tubes, even including plastic capillary tubes.

  15. AGR-3/4 Data Qualification Report for ATR Cycles 151A, 151B, 152A, 152B, 154A, and 154B

    Energy Technology Data Exchange (ETDEWEB)

    Binh T. Pham

    2014-02-01

    This data report provides the qualification status of Advanced Gas Reactor-3/4 (AGR-3/4) fuel irradiation experimental data from Advanced Test Reactor (ATR) Cycles 151A, 151B, 152A, 152B, 154A, and 154B, as recorded in the Nuclear Data Management and Analysis System (NDMAS). Of these cycles, ATR Cycle 152A is a low power cycle that occurred when the ATR core was briefly at low power. The irradiation data are not used for physics and thermal calculation, but the qualification status of these cycle data is still covered in this report. On the other hand, during ATR Cycles 153A (unplanned Outage cycle) and 153B (Power Axial Locator Mechanism [PALM] cycle), the AGR-3/4 was pulled out from the ATR core and stored in the canal to avoid being overheated. Therefore, qualification of the AGR-3/4 irradiation data from these 2 cycles was excluded in this report. By the end of ATR Cycle 154B, AGR-3/4 was irradiated for a total of 264.1 effective full power days. The AGR-3/4 data streams addressed in this report include thermocouple (TC) temperatures, sweep gas data (flow rates, pressure, and moisture content), and Fission Product Monitoring System (FPMS) data (release rates and release-to-birth rate ratios [R/Bs]) for each of the twelve capsules in the AGR-3/4 experiment. The final data qualification status for these data streams is determined by a Data Review Committee (DRC) composed of AGR technical leads, Sitewide Quality Assurance (QA), and NDMAS analysts. The DRC convened on February 12, 2014, reviewed the data acquisition process, and considered whether the data met the requirements for data collection as specified in QA-approved Very High Temperature Reactor (VHTR) Technology Development Office (TDO) data collection plans. The DRC also examined the results of NDMAS data testing and statistical analyses, and confirmed the qualification status of the data as given in this report.

  16. Overview of aerosol properties associated with air masses sampled by the ATR-42 during the EUCAARI campaign (2008

    Directory of Open Access Journals (Sweden)

    S. Crumeyrolle

    2012-04-01

    Full Text Available Within the frame of the European Aerosol Cloud Climate and Air Quality Interactions (EUCAARI project the Météo-France aircraft ATR-42 performed 22 research flights, over central Europe and the North Sea during the intensive observation period in May 2008. For the campaign, the ATR-42 was equipped in order to study aerosol physical, chemical and optical properties, as well as cloud microphysics. During the campaign, continental air masses from Eastern and Western Europe were encountered, along with polar and Scandinavian air masses. For the 22 research flights, retroplume analyses along the flight tracks were performed with FLEXPART in order to classify air masses into five sectors of origin which allows for a qualitative evaluation of emission influence on the respective air parcel.

    In the polluted boundary layer (BL, typical concentrations of particles with diameters larger than 10 nm (N10 are of the order of 5000–6000 cm−3, whereas N10 concentrations of clean air masses were lower than 1300 cm−3. The detection of the largest particle number concentrations occurred in air masses coming from Polar and Scandinavian regions for which an elevated number of nucleation mode (25–28 nm particles was observed and attributed to new particle formation over open sea. In the free troposphere (FT, typical observed N10 are of the order of 900 cm−3 in polluted air masses and 400–600 cm−3 in clean air masses, respectively. In both layers, the chemical composition of submicron aerosol particles is dominated by organic matter and nitrate in polluted air masses, while, sulphate and ammonium followed by organics dominate the submicron aerosols in clean air masses. The highest CCN/CN ratios were observed within the polar air masses while the CCN concentration values are the highest within the polluted air masses.

    Within the five air mass sectors defined

  17. In situ ATR-FTIR study of H2O and D2O adsorption on TiO2 under UV irradiation.

    Science.gov (United States)

    Belhadj, Hamza; Hakki, Amer; Robertson, Peter K J; Bahnemann, Detlef W

    2015-09-21

    The adsorption of water and deuterium oxide on TiO2 surfaces was investigated in the dark as well as under UV(A) irradiation using in situ ATR-FTIR spectroscopy under oxygen and oxygen free conditions. Adsorption of H2O-D2O mixtures revealed an isotopic exchange reaction occurring onto the surface of TiO2 in the dark. Under UV(A) irradiation, the amount of both OH and OD groups was found to be increased by the presence of molecular oxygen. Furthermore, the photocatalytic formation of hydroperoxide under oxygenated condition has been recorded utilizing Attenuated Total Reflection Fourier Transformed Infrared (ATR-FTIR) spectroscopy which appeared as new band at 3483 cm(-1). Different possible mechanisms are discussed in terms of the source of hydroxyl groups formed and/or hydration water on the TiO2 surface for the photocatalytic reaction and photoinduced hydrophilicity. PMID:26266701

  18. ATR-FTIR spectroscopy detects alterations induced by organotin(IV) carboxylates in MCF-7 cells at sub-cytotoxic/-genotoxic concentrations

    CERN Document Server

    Ahmad, Muhammad S; Hussain, Mukhtiar; Hanif, Muhammad; Ali, Saqib; Walsh, Michael J; Martin, Francis L; 10.1186/1757-5036-1-3

    2009-01-01

    The environmental impact of metal complexes such as organotin(IV) compounds is of increasing concern. Genotoxic effects of organotin(IV) compounds (0.01 microg/ml, 0.1 microg/ml or 1.0 microg/ml) were measured using the alkaline single-cell gel electrophoresis (comet) assay to measure DNA single-strand breaks (SSBs) and the cytokinesis-block micronucleus (CBMN) assay to determine micronucleus formation. Biochemical-cell signatures were also ascertained using attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy. In the comet assay, organotin(IV) carboxylates induced significantly-elevated levels of DNA SSBs. Elevated micronucleus-forming activities were also observed. Following interrogation using ATR-FTIR spectroscopy, infrared spectra in the biomolecular range (900 cm-1 - 1800 cm-1) derived from orga...

  19. Caracterització fenotípica i assaig terapèutic en models murins transgènics d'atròfia muscular espinal

    OpenAIRE

    Dachs i Cabanas, Elisabet

    2012-01-01

    L’atròfia muscular espinal (AME) és una malaltia d’origen genètic que afecta, majoritàriament a la població infantil. La malaltia cursa amb una mort de les motoneurones  i atròfia muscular. El gen implicat és el survival motor neuron (SMN) que està delecionat en un 95% dels casos. El nostre estudi està dividit en dues parts: 1- l’aprofundiment de les alteracions musculars en dos models animals murins transgènics que pateixen les formes més greus d’AME (Tipus 1-2) i 2- estudi dels possibles e...

  20. Understanding the Crystallinity Indices Behavior of Burned Bones and Teeth by ATR-IR and XRD in the Presence of Bioapatite Mixed with Other Phosphate and Carbonate Phases

    Directory of Open Access Journals (Sweden)

    Giampaolo Piga

    2016-01-01

    Full Text Available We have critically investigated the ATR-IR spectroscopy data behavior of burned human teeth as opposed to the generally observed behavior in human bones that were subjected to heat treatment, whether deliberate or accidental. It is shown that the deterioration of the crystallinity index (CI behavior sometimes observed in bones subjected to high temperature appears to be of higher frequency in the case of bioapatite from teeth. This occurs because the formation of the β-tricalcium phosphate (β-TCP phase, otherwise known as whitlockite, clearly ascertained by the X-ray diffraction (XRD patterns collected on the same powdered specimens investigated by ATR-IR. These results point to the need of combining more than one physicochemical technique even if apparently well suitable, in order to verify whether the assumed conditions assessed by spectroscopy are fully maintained in the specimens after temperature and/or mechanical processing.

  1. A Feasibility Study to Determine Cooling Time and Burnup of ATR Fuel Using a Nondestructive Technique and Three Types of Gamma-ray Detectors

    Energy Technology Data Exchange (ETDEWEB)

    Jorge Navarro; Rahmat Aryaeinejad,; David W. Nigg

    2011-05-01

    A Feasibility Study to Determine Cooling Time and Burnup of ATR Fuel Using a Nondestructive Technique1 Rahmat Aryaeinejad, Jorge Navarro, and David W Nigg Idaho National Laboratory Abstract Effective and efficient Advanced Test Reactor (ATR) fuel management require state of the art core modeling tools. These new tools will need isotopic and burnup validation data before they are put into production. To create isotopic, burn up validation libraries and to determine the setup for permanent fuel scanner system a feasibility study was perform. The study consisted in measuring short and long cooling time fuel elements at the ATR canal. Three gamma spectroscopy detectors (HPGe, LaBr3, and HPXe) and two system configurations (above and under water) were used in the feasibility study. The first stage of the study was to investigate which detector and system configuration would be better suited for different scenarios. The second stage of the feasibility study was to create burnup and cooling time calibrations using experimental isotopic data collected and ORIGEN 2.2 burnup data. The results of the study establish that a better spectra resolution is achieve with an above the water configuration and that three detectors can be used in the permanent fuel scanner system for different situations. In addition it was conclude that a number of isotopic ratios and absolute measurements could be used to predict ATR fuel burnup and cooling times. 1This work was supported by the U.S. Depart¬ment of Energy (DOE) under Battelle Energy Alliance, LLC Contract No. DE-AC07-05ID14517.

  2. Fourier transform infrared-attenuated total reflectance (FTIR-ATR) spectroscopy and chemometric techniques for the determination of adulteration in petrodiesel/biodiesel blends

    OpenAIRE

    Armando Guerrero Peña; Francisco Anguebes Franseschi; Mepivoseth Castelán Estrada; Victorino Morales Ramos; Reyes García Zarracino; José C. Zavala Loría; Atl V. Córdova Quiroz

    2014-01-01

    We propose an analytical method based on fourier transform infrared-attenuated total reflectance (FTIR-ATR) spectroscopy to detect the adulteration of petrodiesel and petrodiesel/palm biodiesel blends with African crude palm oil. The infrared spectral fingerprints from the sample analysis were used to perform principal components analysis (PCA) and to construct a prediction model using partial least squares (PLS) regression. The PCA results separated the samples into three groups, allowing id...

  3. AGR-2 Data Qualification Report for ATR Cycles 147A, 148A, 148B, and 149A

    Energy Technology Data Exchange (ETDEWEB)

    Michael L. Abbott; Keith A. Daum

    2011-08-01

    This report presents the data qualification status of fuel irradiation data from the first four reactor cycles (147A, 148A, 148B, and 149A) of the on-going second Advanced Gas Reactor (AGR-2) experiment as recorded in the NGNP Data Management and Analysis System (NDMAS). This includes data received by NDMAS from the period June 22, 2010 through May 21, 2011. AGR-2 is the second in a series of eight planned irradiation experiments for the AGR Fuel Development and Qualification Program, which supports development of the very high temperature gas-cooled reactor (VHTR) under the Next Generation Nuclear Plant (NGNP) Project. Irradiation of the AGR-2 test train is being performed at the Advanced Test Reactor (ATR) at the Idaho National Laboratory (INL) and is planned for 600 effective full power days (approximately 2.75 calendar years) (PLN-3798). The experiment is intended to demonstrate the performance of UCO (uranium oxycarbide) and UO2 (uranium dioxide) fuel produced in a large coater. Data qualification status of the AGR-1 experiment was reported in INL/EXT-10-17943 (Abbott et al. 2010).

  4. Quantification of pure refined olive oil adulterant in extra virgin olive oil using diamond cell atr-ftir spectroscopy

    International Nuclear Information System (INIS)

    The present study depicts spectroscopic method development to deliver a rapid, simple and reproducible quantification of pure refined olive oil (PROO) adulterant in extra virgin olive oil (EVOO) using partial least square (PLS) regression (statistical parameter). Single bounce attenuated total reflectance (SB-ATR) Fourier transform infrared (FTIR) was choice in the developed method. Blended standards of PROO and EVOO were obtained by their weight by weight percentage and the values were used to construct calibration curves for quantification. The optimum regression values (i.e. >0.99) were achieved using the combined frequencies of 3105-2761, 1838-1687, and 1482-440 cm-1 with regression coefficients (R2) 0.99718 and achieved residual mean square error of calibration (RMSEC) 1.40% w/w. To determine the suitability of developed method principal component spectra (PCS) diagnostic was also used. The results of the present study prove that the developed methods reported in preceding studies can be good option for more rapid and accurate determination of PROO adulteration in EVOO. (author)

  5. Improving batch cooling seeded crystallization of an organic weed-killer using on-line ATR FTIR measurement of supersaturation

    Science.gov (United States)

    Lewiner, F.; Févotte, G.; Klein, J. P.; Puel, F.

    2001-06-01

    In previous papers, a method for the calibration of ATR FTIR measurement of dissolved solid concentration during cooling crystallizations was presented, and efficient basic procedures for the determination of solubility and metastability curves were described. The present paper reports new experimental results. The crystallization of an organic product is studied thanks to on-line FTIR measurements of supersaturation and off-line crystal size distribution (CSD) determinations. The obtained information is shown to potentially allow a deeper examination of major industrial issues such as the reduction of batch-to-batch variations and the improvement of the CSD of the final particles. A particular attention is focused on the determination of appropriate seeding parameters such as the cooling rate of seeded slurries, the temperature of introduction and the amount of seed. The results obtained demonstrate that, although any modelling approach is outside the scope of this paper, the analysis of the measured supersaturation profiles allows some interpretation of mechanisms governing the final CSD, and consequently, the proposal of improved operating parameters.

  6. ATR-FTIR study of water in Nafion membrane combined with proton conductivity measurements during hydration/dehydration cycle.

    Science.gov (United States)

    Kunimatsu, Keiji; Bae, Byungchan; Miyatake, Kenji; Uchida, Hiroyuki; Watanabe, Masahiro

    2011-04-21

    We have conducted combined time-resolved attenuated total reflection Fourier transform infrared (ATR-FTIR) and proton conductivity measurements of Nafion NRE211 membrane during hydration/dehydration cycles at room temperature. Conductivity change was interpreted in terms of different states of water in the membrane based on its δ(HOH) vibrational spectra. It was found that hydration of a dry membrane leads first to complete dissociation of the sulfonic acid groups to liberate hydrated protons, which are isolated from each other and have δ(HOH) vibrational frequency around 1740 cm(-1). The initial hydration is not accompanied by a significant increase of the proton conductivity. Further hydration gives rise to a rapid increase of the conductivity in proportion to intensity of a new δ(HOH) band around 1630 cm(-1). This was interpreted in terms of formation of channels of weakly hydrogen-bonded water to combine the isolated hydrophilic domains containing hydrated protons and hydrated sulfonate ions produced during the initial stage of hydration. Upon dehydration, proton conductivity drops first very rapidly due to loss of the weakly hydrogen bonded water from the channels to leave hydrophilic domains isolated in the membrane. Dehydration of the protons proceeds very slowly after significant loss of the proton conductivity.

  7. Characterization of nasal paraffinoma following septorhinoplasty by attenuated total reflection--fourier transform infrared spectroscopy (ATR-FTIR).

    Science.gov (United States)

    Gil-Carcedo, Luis M; Martín-Gil, Jesús; Gil-Carcedo, Elisa S; Vallejo, Luis A; Martín-Gil, Francisco J

    2006-06-01

    Vaseline and paraffin have been injected into various parts of the body. Vaselinoma and paraffinoma are well-described complications, despite which nasal packing with Vaseline gauze is still common in the management of epistaxis, after rhinoplasty, endonasal surgery, to control bleeding and prevent synechiae or restenosis. Our aim is to highlight this complication, propose a safe method for its diagnosis and establish guidelines for its prevention. We report two cases of paraffinoma occurring after rhinoplasty and discuss prevention of this rare but serious complication, and suggest an alternative dressing. Attenuated total reflection (ATR) FI7R spectra have proven to be a definitive characterising tool for surgical extracts, guaranteeing detection of mineral products that histology does not offer. For these lesions we propose the name "petroleum oilomas" which we feel to be more appropriate than the more commonly used paraffinomas. Relevance of the work: a description of an innovative and safe method of diagnosis, and proposal of a procedure for postrhinoplasty packing (without mineral oils) to avoid this complication. PMID:16792177

  8. Evaluating drug delivery with salt formation: Drug disproportionation studied in situ by ATR-FTIR imaging and Raman mapping.

    Science.gov (United States)

    Ewing, Andrew V; Wray, Patrick S; Clarke, Graham S; Kazarian, Sergei G

    2015-01-01

    Two different vibrational spectroscopic approaches, ATR-FTIR spectroscopic imaging and Raman mapping, were used to investigate the components within a tablet containing an ionised drug during dissolution experiments. Delivering certain drugs in their salt form is a method that can be used to improve the bioavailability and dissolution of the poorly aqueous soluble materials. However, these ionised species have a propensity to covert back to their thermodynamically favourable free acid or base forms. Dissolution experiments of the ionised drug in different aqueous media resulted in conversion to the more poorly soluble free acid form, which is detrimental for controlled drug release. This study investigates the chemical changes occurring to formulations containing a development ionised drug (37% by weight), in different aqueous pH environments. Firstly, dissolution in a neutral medium was studied, showing that there was clear release of ionised monosodium form of the drug from the tablet as it swelled in the aqueous medium. There was no presence of any drug in the monohydrate free acid form detected in these experiments. Dissolution in an acidic (0.1M HCl) solution showed disproportionation forming the free acid form. Disproportionation occurred rapidly upon contact with the acidic solution, initially resulting in a shell of the monohydrate free acid form around the tablet edges. This slowed ingress of the solution into the tablet before full conversion of the ionised form to the free acid form was characterised in the spectroscopic data. PMID:25910459

  9. Evaluation of Salmon Adhesion on PET-Metal Interface by ATR, FT-IR, and Raman Spectroscopy

    Directory of Open Access Journals (Sweden)

    E. Zumelzu

    2015-01-01

    Full Text Available The material employed in this study is an ecoefficient, environmentally friendly, chromium (VI-free (noncarcinogenic metal polymer. The originality of the research lies in the study of the effect of new production procedures of salmon on metal packaging with multilayer polyethylene terephthalate (PET polymer coatings. Our hypothesis states that the adhesion of postmortem salmon muscles to the PET polymer coating produces surface and structural changes that affect the functionality and limit the useful life of metal containers, compromising therefore their recycling capacity as ecomaterials. This work is focused on studying the effects of the biochemical changes of postmortem salmon on the PET coating and how muscle degradation favors adhesion to the container. The experimental design considered a series of laboratory tests of containers simulating the conditions of canned salmon, chemical and physical tests of food-contact canning to evaluate the adhesion, and characterization of changes in the multilayer PET polymer by electron microscopy, ATR, FT-IR, and Raman spectroscopy analyses. The analyses determined the effect of heat treatment of containers on the loss of freshness of canned fish and the increased adhesion to the container wall, and the limited capability of the urea treatment to remove salmon muscle from the container for recycling purposes.

  10. The Conference Proceedings of the 2001 Air Transport Research Society (ATRS) of the WCTR Society. Volume 2

    Science.gov (United States)

    Lee, Yeong-Heok (Editor); Bowen, Brent D. (Editor); Tarry, Scott E. (Editor)

    2001-01-01

    The ATRS held its 5th Annual conference at the City University of Hong Kong Campus in July 2001. The conference was a success with nearly 140 participants including 70 presenters. Titles that comprise Volume 2 include: Intelligent Airport Gate Assignment System; A Study on the Effects of the Personality Compatibility to the Job Performance; ITS/CVO Application for Air cargo Transportation in Korea; An Airport as a Logistics and Economic Hub: The Case of Incheon International Airport; The Impact Of Aviation Safety over the Consumer's Behavior; The Integration of China and Taiwan Air Networks for Direct Air Cargo Services; Quality perception and carrier choice in Civil Aviation; Future Trends in Business Travel Decision Making; Cooperation Among German Airports in Europe; Inbound and Outbound Air Passenger Traffic Forecasting between the United States and Selected Asian countries; An Evaluation of Alternative Facilities for Airport Redevelopment using Fuzzy Linguistic Approach; Economic Analysis of Airline Alliances; The Aviation Cooperation between the two Koreas Preparing for the Reunification of the Peninsula; and A Study on the Air Transport Cooperation in Northeast Asia between China, Japan and Korea.

  11. Determination of surface concentrations of individual molecule-layers used in nanoscale biosensors by in situ ATR-FTIR spectroscopy

    KAUST Repository

    Punzet, Manuel

    2012-01-01

    For the development of nanowire sensors for chemical and medical detection purposes, the optimal functionalization of the surface is a mandatory component. Quantitative ATR-FTIR spectroscopy was used in situ to investigate the step-by-step layer formation of typical functionalization protocols and to determine the respective molecule surface concentrations. BSA, anti-TNF-α and anti-PSA antibodies were bound via 3-(trimethoxy)butylsilyl aldehyde linkers to silicon-oxide surfaces in order to investigate surface functionalization of nanowires. Maximum determined surface concentrations were 7.17 × 10 -13 mol cm -2 for BSA, 1.7 × 10 -13 mol cm -2 for anti-TNF-α antibody, 6.1 × 10 -13 mol cm -2 for anti-PSA antibody, 3.88 × 10 -13 mol cm -2 for TNF-α and 7.0 × 10 -13 mol cm -2 for PSA. Furthermore we performed antibody-antigen binding experiments and determined the specific binding ratios. The maximum possible ratio of 2 was obtained at bulk concentrations of the antigen in the μg ml -1 range for TNF-α and PSA. © 2012 The Royal Society of Chemistry.

  12. Mismatch repair-dependent G2 checkpoint induced by low doses of SN1 type methylating agents requires the ATR kinase.

    Science.gov (United States)

    Stojic, Lovorka; Mojas, Nina; Cejka, Petr; Di Pietro, Massimiliano; Ferrari, Stefano; Marra, Giancarlo; Jiricny, Josef

    2004-06-01

    S(N)1-type alkylating agents represent an important class of chemotherapeutics, but the molecular mechanisms underlying their cytotoxicity are unknown. Thus, although these substances modify predominantly purine nitrogen atoms, their toxicity appears to result from the processing of O(6)-methylguanine ((6Me)G)-containing mispairs by the mismatch repair (MMR) system, because cells with defective MMR are highly resistant to killing by these agents. In an attempt to understand the role of the MMR system in the molecular transactions underlying the toxicity of alkylating agents, we studied the response of human MMR-proficient and MMR-deficient cells to low concentrations of the prototypic methylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). We now show that MNNG treatment induced a cell cycle arrest that was absolutely dependent on functional MMR. Unusually, the cells arrested only in the second G(2) phase after treatment. Downstream targets of both ATM (Ataxia telangiectasia mutated) and ATR (ATM and Rad3-related) kinases were modified, but only the ablation of ATR, or the inhibition of CHK1, attenuated the arrest. The checkpoint activation was accompanied by the formation of nuclear foci containing the signaling and repair proteins ATR, the S(*)/T(*)Q substrate, gamma-H2AX, and replication protein A (RPA). The persistence of these foci implied that they may represent sites of irreparable damage. PMID:15175264

  13. Study in vitro of Er,Cr:YSGG laser effects in bone tissue by ATR-FTIR spectroscopy

    International Nuclear Information System (INIS)

    Laser proves to be, more and more, an effective tool for helping health professionals, being intensively used in ophthalmological and odontological procedures. In particular, high-density, infrared emitting lasers have great potential in cutting mineralized biological hard tissues, given their high absorption by hydroxyapatite and water, these tissues' main components. In comparison to mechanical instruments, laser presents a series of advantages, namely, smaller damage to the remaining tissue and promotion of homeostatic effect, apart from making it possible to execute procedures in areas with difficult access. However, for an efficient and safe use of this technique, it is necessary to know the effects of the laser irradiation on the tissue. The Fourier transform infrared (FTIR) technique is heavily used in the study of organic materials, because apart from making it possible to identify the materials' components, it also allows to prepare a semi quantitative analysis. This work aims to establish the ATR-FTIR technique in the characterization of natural and irradiated osseous tissue, and to verify the possible chemical and structural changes caused by irradiation. Firstly, the best conditions for the obtainment of bone sample spectra were determined. Then, bone samples, irradiated with the Er,Cr:YSGG (2,78 μm) infrared emitting laser (adjusted with different energy densities) were analyzed alongside with natural bone samples. It has been verified that the technique is effective in the bone tissue characterization, and that it is possible to observe the chemical changes caused by the temperature rise due to laser irradiation. It has been observed a gradual organic material loss as the energy density goes up. These results are the first steps in testing the Er,Cr:YSGG laser efficacy as a cutting tool, a pivotal aspect of its consolidation in clinical procedures. (author)

  14. XPS, SIMS and FTIR-ATR characterization of boronized graphite from the thermonuclear plasma device RFX-mod

    Science.gov (United States)

    Ghezzi, F.; Laguardia, L.; Caniello, R.; Canton, A.; Dal Bello, S.; Rais, B.; Anderle, M.

    2015-11-01

    In this paper the characterization of a thin (tens of nanometers) boron layer on fine grain polycrystalline graphite substrate is presented. The boron film is used as conditioning technique for the full graphite wall of the Reversed Field eXperiment-modified (RFX-mod) experiment, a device for the magnetic confinement of plasmas of thermonuclear interest. Aim of the present analysis is to enlighten the chemical structure of the film, the trapping mechanism that makes it a getter for oxygen and hydrogen and the reason of its loss of effectiveness after exposure to about 100 s of hydrogen plasma. X-ray Photoelectron Spectroscopy (XPS), Secondary Ions Mass Spectrometry (SIMS) and Fourier Transform Infra Red spectroscopy in combination with the Attenuated Total Reflectance (FTIR-ATR) were used to obtain the structure and the chemical composition of graphitic samples as coated or coated and subsequently exposed to hydrogen plasma after boron deposition. The boron layers on the only coated samples were found to be amorphous hydrogenated boron carbide plus a variety of bonds like B-B, B-H, B-O, B-OH, C-C, C-H, C-O, C-OH. Both the thickness and the homogeneity of the layers were found to depend on the distance of the sample from the anode during the deposition. The samples contained oxygen along the layer thickness, at level of 5%, bound to boron. The gettering action of the boron is therefore already active during the deposition itself. The exposure to plasma caused erosion of the boron film and higher content of H and O bound to boron throughout the whole thickness. The interaction of the B layer with plasma is therefore a bulk phenomenon.

  15. ATR-FTIR and UV-Vis Spectroscopic Studies of Aqueous U(IV)-oxalate Complexes under Mild Acidic Conditions

    Energy Technology Data Exchange (ETDEWEB)

    Cha, Wansik; Jung, Euo Chang; Cho, Hyeryun; Park, Yangsoon; Ha, Yeongkeong [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2014-05-15

    The redox transformation process between U(VI) and U(IV) likely involves the participation of soluble or dissolved U(IV) species, such as U(IV)-hydroxo compounds and organic/inorganic ligand complexes. However, their role in the redox process has not been well documented, partly due to the ready oxidation of soluble U(IV) species, and partly due to the assumption that soluble or dissolved forms of U(IV) account for only a minor fraction of uranium in groundwater systems. In this study, a bidentate chelate ligand, oxalate (Ox) was selected to examine the complexation behaviors of U(IV) and ultimately its impact on the U(IV) solubility in mildly acidic solutions. Although some early studies reported that oxalate and pyrophosphate, i. e., multivalent anions, can form soluble U(IV) complexes, the related thermodynamic data and evidences for chemical speciation are very scarce. In our previous work, the U(IV)-Ox 1:1 complex was identified by monitoring the gradual transition of the characteristic absorption spectrum of U(OH){sup 3+} to that of UOx{sup 2+} upon the addition of oxalate at pH 1.6.2.0. This work aims to further provide spectroscopic evidence for the formation of multi-ligand complexes, i. e., U(Ox)n{sup 4-2n} (n ≥ 2) at pH 2-5 using attenuated total reflectance (ATR)- FTIR spectroscopy and UV-Vis absorption spectroscopy. The solid phase of U(IV)-Ox complex system was also characterized through an XRD analysis. Analysis of the FTIR spectra is found to be useful to determine the complexation stoichiometry and to obtain the structural information of the complexes. The outcome of the spectroscopic analysis for the multi-ligand complexation equilibria will be discussed in detail.

  16. MCFC-based marine APU: Comparison between conventional ATR and cracking coupled with SR integrated inside the stack pressurized vessel

    Energy Technology Data Exchange (ETDEWEB)

    Bensaid, S.; Specchia, S.; Saracco, G.; Specchia, V. [Materials Science and Chemical Engineering Department, Politecnico di Torino, Corso Duca degli Abruzzi, 24 - 10129 Torino (Italy); Federici, F. [Ansaldo Fuel Cells S.p.A., Corso Perrone 25, 16152 Genova (Italy)

    2009-02-15

    In the present work the implementation of MCFCs as auxiliary power units on-board large vessels, such as cruising, passengers or commercial, ships was investigated. The MCFC stack was designed to supply 500 kW{sub e} and was fed with diesel oil undergoing a reforming process. The system modelling of the plant was performed in steady-state and aimed at assessing the power efficiency for different reforming strategies, process configurations and constituting items thermal integrations. The code Matlab/Simulink was used to this end. Two major fuel processing strategies were examined: ''auto-thermal reforming'' and ''inside vessel steam reforming''. The latter consisted of a pre-reforming unit in which the liquid fuel underwent a catalytic cracking in mild conditions; subsequently, the resulting gas mixture made of light hydrocarbons was mixed with steam and fed into a steam reformer inside the MCFC stack vessel, where conversion to syngas occurred. Due to the high temperature (650 C) exothermic level of MCFC, the stack was compatible with a syngas steam reforming production thermally self sustained. This allowed to increase the global electrical efficiency from 32.7% (for the ATR-based system) up to 44.6%. The process was then designed aiming at increasing the overall efficiency by thermally integrating the outlet flue gases with the pre-heating section. This lead to efficiencies equal to 39.1% and 50.6% for the ''auto-thermal reforming'' and ''inside vessel steam reforming'', respectively. Finally, the process was upgraded from an auxiliary power unit (APU) to a combined heat and power unit (CHP), since the residual heat in the flue gases was recovered for heating purposes (sanitary water production) and the demineralised water recirculation was implemented to reduce the water make-up and the process environmental footprint. (author)

  17. Kinetics study of heterogeneous reactions of ozone with erucic acid using an ATR-IR flow reactor.

    Science.gov (United States)

    Leng, Chunbo; Hiltner, Joseph; Pham, Hai; Kelley, Judas; Mach, Mindy; Zhang, Yunhong; Liu, Yong

    2014-03-01

    The ozone initiated heterogeneous oxidation of erucic acid (EA) thin film was investigated using a flow system combined with attenuated total reflection infrared spectroscopy (ATR-IR) over wide ranges of ozone concentrations (0.25-60 ppm), thin film thickness (0.1-1.0 μm), temperatures (263-298 K), and relative humidities (0-80% RH) for the first time. Pseudo-first-order rate constants, kapp, and overall reactive uptake coefficients, γ, were obtained through changes in the absorbance of C[double bond, length as m-dash]O stretching bands at 1695 cm(-1), which is assigned to the carbonyl group in carboxylic acid. Results showed that the reaction followed the Langmuir-Hinshelwood mechanism and kapp was largely dominated by surface reaction over bulk phase reaction. In addition, both the kapp and the γ values showed very strong temperature dependences (∼two orders of magnitude) over the temperature range; in contrast, they only slightly increased with increasing RH values from 0-80%. According to the kapp values as a function of temperature, the activation energy for the heterogeneous reaction was estimated to be 80.6 kJ mol(-1). Our results have suggested that heterogeneous reactions between ozone and unsaturated solid surfaces likely have a substantially greater temperature dependence than liquid ones. Moreover, the hygroscopic properties of EA thin films before and after exposure to ozone were also studied by measurement of water uptake. Based on the hygroscopicity data, the insignificant RH effect on reaction kinetics was probably due to the relatively weak water uptake by the unreacted and reacted EA thin films.

  18. Molecular level investigations of phosphate sorption on corundum (α-Al2O3) by 31P solid state NMR, ATR-FTIR and quantum chemical calculation

    Science.gov (United States)

    Li, Wei; Pierre-Louis, Andro-Marc; Kwon, Kideok D.; Kubicki, James D.; Strongin, Daniel R.; Phillips, Brian L.

    2013-04-01

    Phosphate sorption at the corundum (α-Al2O3)/water interface was investigated as a function of phosphate concentration (0.1-1 mM) and pH (3-11) by 31P solid state NMR spectroscopy, ATR-FTIR, and quantum chemical calculation. The 31P NMR spectra indicate that under these experimental conditions phosphate adsorbs onto corundum mainly as inner-sphere complexes that yield a peak at δP = -2.8 ppm with full width at half maximum (FWHM) of 9.2 ppm, with a small amount aluminum phosphate surface precipitates as suggested by an NMR signal observed from δP = -12 to -20 ppm. We employed 31P{27Al} rotational echo adiabatic passage double resonance (REAPDOR) to further analyze the phosphate adsorption samples prepared at pH 5 and 9 in order to determine the phosphate/Al coordination. To aid interpretation of the NMR data, a series of bidentate and monodentate structural models of phosphate adsorbed on corundum (0 0 1) and (0 1 2) surfaces were calculated using density function theory (DFT). By comparing the experimental REAPDOR curves and those simulated from these models, we can assign the dominant peaks to bidentate binuclear surface complexes. Formation of bidentate binuclear surface complexes is supported by the ATR/FTIR spectra combined with DFT calculation, which further suggests a mixture of non-protonated bidentate and mono-protonated bidentate surface complexes on the corundum surface at pH 5. At pH 9, both NMR and ATR/FTIR indicate the formation of bidentate surface complexes on corundum surface.

  19. ATR-p53 restricts homologous recombination in response to replicative stress but does not limit DNA interstrand crosslink repair in lung cancer cells.

    Directory of Open Access Journals (Sweden)

    Bianca M Sirbu

    Full Text Available Homologous recombination (HR is required for the restart of collapsed DNA replication forks and error-free repair of DNA double-strand breaks (DSB. However, unscheduled or hyperactive HR may lead to genomic instability and promote cancer development. The cellular factors that restrict HR processes in mammalian cells are only beginning to be elucidated. The tumor suppressor p53 has been implicated in the suppression of HR though it has remained unclear why p53, as the guardian of the genome, would impair an error-free repair process. Here, we show for the first time that p53 downregulates foci formation of the RAD51 recombinase in response to replicative stress in H1299 lung cancer cells in a manner that is independent of its role as a transcription factor. We find that this downregulation of HR is not only completely dependent on the binding site of p53 with replication protein A but also the ATR/ATM serine 15 phosphorylation site. Genetic analysis suggests that ATR but not ATM kinase modulates p53's function in HR. The suppression of HR by p53 can be bypassed under experimental conditions that cause DSB either directly or indirectly, in line with p53's role as a guardian of the genome. As a result, transactivation-inactive p53 does not compromise the resistance of H1299 cells to the interstrand crosslinking agent mitomycin C. Altogether, our data support a model in which p53 plays an anti-recombinogenic role in the ATR-dependent mammalian replication checkpoint but does not impair a cell's ability to use HR for the removal of DSB induced by cytotoxic agents.

  20. ATR-Chk1-APC/C-dependent stabilization of Cdc7-ASK (Dbf4) kinase is required for DNA lesion bypass under replication stress

    DEFF Research Database (Denmark)

    Yamada, M.; Watanabe, K.; Mistrik, M.;

    2013-01-01

    replication. Stalled DNA replication evoked stabilization of the Cdc7-ASK (Dbf4) complex in a manner dependent on ATR-Chk1-mediated checkpoint signaling and its interplay with the anaphase-promoting complex/cyclosomeCdh1 (APC/C) ubiquitin ligase. Mechanistically, Chk1 kinase inactivates APC/C through...... degradation of Cdh1 upon replication block, thereby stabilizing APC/C substrates, including Cdc7-ASK (Dbf4). Furthermore, motif C of ASK (Dbf4) interacts with the N-terminal region of RAD18 ubiquitin ligase, and this interaction is required for chromatin binding of RAD18. Impaired interaction of ASK (Dbf4...

  1. Spectral features of guanidinium-carboxylate salt bridges. The combined ATR-IR and theoretical studies of aqueous solution of guanidinium acetate

    Science.gov (United States)

    Levina, Elena O.; Lokshin, Boris V.; Mai, Bich D.; Vener, Mikhail V.

    2016-08-01

    The spectrum of guanidinium acetate in aqueous solution has been recorded by attenuated total reflectance infrared spectroscopy (ATR-IR). Assignments of the bands have been done using the polarizable continuum model (PCM). Three IR intensive bands at 1670, 1550, and 1410 cm-1 are associated with stretching and bending vibrations of the groups forming a ring of six heavy atoms of the bidentate configuration of guanidinium acetate. The relatively weak broad band near 2200 cm-1 is tentatively assigned to the stretching vibration of the Nsbnd H⋯O fragment of the hydrogen-bonded ion pairs.

  2. Bacterial and abiotic decay in waterlogged archaeological Picea abies (L.) Karst studied by confocal Raman imaging and ATR-FTIR spectroscopy

    DEFF Research Database (Denmark)

    Pedersen, Nanna Bjerregaard; Gierlinger, Notburga; Thygesen, Lisbeth Garbrecht

    2015-01-01

    contained regions with intensities lower than sound S2 layers up to intensity values as high as the compound middle lamella (CML). CRI revealed strong depletion of carbohydrates in RM which indicated that EB are able to utilise the carbohydrate fraction of the cell wall effectively. Raman bands assigned......Waterlogged archaeological Norway spruce [Picea abies (L.) Karst] poles were studied by means of confocal Raman imaging (CRI) and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) analysis to determine lignin and polysaccharide composition and distribution in the cell...

  3. Radiation Induced Aging Effects in Polymeric Cable Insulators at CERN. Compilation of the DSC and ATR-FTIR data on irradiated CERN cables.

    CERN Document Server

    Sorin, I

    2009-01-01

    This second part of the Report on Radiation Induced Aging Effects in Polymeric Cable Insulators at CERN [1] summarizes in a tabulated form all the experimental DSC results obtained in this work, concerning the life-time evaluation of the selected CERN cables irradiated in different conditions. Several examples of DSC curves and ATR-FTIR spectra were included to support the future polymeric materials analysis. Information on the experimental conditions, the instruments, the basic concept of life-time evaluation using the DSC data, as well as the examples of data processing and interpretation are presented and discussed in reference [1].

  4. Fuentes de variabilidad en el diagnóstico de gastritis atrófica multifocal asociada con la infección por Helicobacter pylori

    OpenAIRE

    Luis Eduardo Bravo; Juan Carlos Bravo; José Luis Realpe

    2008-01-01

    Introducción: El mapeo de las diferentes regiones del estómago y el número de fragmentos de mucosa gástrica disponibles para evaluación histopatológica son fuentes importantes de variación en el momento de clasificar y hacer la gradación de la gastritis crónica. Objetivos: Estimar la sensibilidad del número de fragmentos de mucosa gástrica necesarios para establecer los diagnósticos de gastritis atrófica con metaplasia intestinal (MI), displasia y estado de infección por Helicobacter pylori. ...

  5. Kinetic and Conformational Insights of Protein Adsorption onto Montmorillonite Revealed Using in Situ ATR-FTIR/2D-COS.

    Science.gov (United States)

    Schmidt, Michael P; Martínez, Carmen Enid

    2016-08-01

    Protein adsorption onto clay minerals is a process with wide-ranging impacts on the environmental cycling of nutrients and contaminants. This process is influenced by kinetic and conformational factors that are often challenging to probe in situ. This study represents an in situ attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopic investigation of the adsorption of a model protein (bovine serum albumin (BSA)) onto a clay mineral (montmorillonite) at four concentrations (1.50, 3.75, 7.50, and 15.0 μM) under environmentally relevant conditions. At all concentrations probed, FTIR spectra show that BSA readily adsorbs onto montmorillonite. Adsorption kinetics follow an Elovich model, suggesting that primary limitations on adsorption rates are surface-related heterogeneous energetic restrictions associated with protein rearrangement and lateral protein-protein interaction. BSA adsorption onto montmorillonite fits the Langmuir model, yielding K = 5.97 × 10(5) M(-1). Deconvolution and curve fitting of the amide I band at the end of the adsorption process (∼120 min) shows a large extent of BSA unfolding upon adsorption at 1.50 μM, with extended chains and turns increasing at the expense of α-helices. At higher concentrations/surface coverages, BSA unfolding is less pronounced and a more compact structure is assumed. Two-dimensional correlation spectroscopic (2D-COS) analysis reveals three different pathways corresponding to adsorbed conformations. At 1.50 μM, adsorption increases extended chains, followed by a loss in α-helices and a subsequent increase in turns. At 3.75 μM, extended chains decrease and then aggregated strands increase and side chains decrease, followed by a decrease in turns. With 7.50 and 15.0 μM BSA, the loss of side-chain vibrations is followed by an increase in aggregated strands and a subsequent decrease in turns and extended chains. Overall, the BSA concentration and resultant surface coverage have a profound

  6. Human monocytes undergo excessive apoptosis following temozolomide activating the ATM/ATR pathway while dendritic cells and macrophages are resistant.

    Directory of Open Access Journals (Sweden)

    Martina Bauer

    Full Text Available Immunodeficiency is a severe therapy-limiting side effect of anticancer chemotherapy resulting from sensitivity of immunocompetent cells to DNA damaging agents. A central role in the immune system is played by monocytes that differentiate into macrophages and dendritic cells (DCs. In this study we compared human monocytes isolated from peripheral blood and cytokine matured macrophages and DCs derived from them and assessed the mechanism of toxicity of the DNA methylating anticancer drug temozolomide (TMZ in these cell populations. We observed that monocytes, but not DCs and macrophages, were highly sensitive to the killing effect of TMZ. Studies on DNA damage and repair revealed that the initial DNA incision was efficient in monocytes while the re-ligation step of base excision repair (BER can not be accomplished, resulting in an accumulation of DNA single-strand breaks (SSBs. Furthermore, monocytes accumulated DNA double-strand breaks (DSBs following TMZ treatment, while DCs and macrophages were able to repair DSBs. Monocytes lack the DNA repair proteins XRCC1, ligase IIIα and PARP-1 whose expression is restored during differentiation into macrophages and DCs following treatment with GM-CSF and GM-CSF plus IL-4, respectively. These proteins play a key role both in BER and DSB repair by B-NHEJ, which explains the accumulation of DNA breaks in monocytes following TMZ treatment. Although TMZ provoked an upregulation of XRCC1 and ligase IIIα, BER was not enhanced likely because PARP-1 was not upregulated. Accordingly, inhibition of PARP-1 did not sensitize monocytes, but monocyte-derived DCs in which strong PARP activation was observed. TMZ induced in monocytes the DNA damage response pathways ATM-Chk2 and ATR-Chk1 resulting in p53 activation. Finally, upon activation of the Fas-receptor and the mitochondrial pathway apoptosis was executed in a caspase-dependent manner. The downregulation of DNA repair in monocytes, resulting in their selective

  7. FTIR-ATR study of water uptake and diffusion through ion-selective membranes based on poly(acrylates) and silicone rubber.

    Science.gov (United States)

    Sundfors, Fredrik; Lindfors, Tom; Höfler, Lajos; Bereczki, Róbert; Gyurcsányi, Róbert E

    2009-07-15

    For the first time, FTIR-ATR spectroscopy was used to study the water uptake and its diffusion in ion-selective membranes (ISMs) based on poly(acrylates) (PAs) and silicone rubber (SR), which are emerging materials for the fabrication of ISMs for ultratrace analysis. Three different types of PA membranes were studied, consisting of copolymers of methyl methacrylate with n-butyl acrylate, decyl methacrylate, or isodecyl acrylate. Numerical simulations with the finite difference method showed that in most cases the water uptake of the PA and SR membranes could be described with a model consisting of two diffusion coefficients. The diffusion coefficients of the PA membranes were approximately 1 order of magnitude lower than those of plasticized poly(vinyl chloride) (PVC)-based ISMs and only slightly influenced by the membrane matrix composition. However, the simulations indicated that during longer contact times, the water uptake of PA membranes was considerably higher than that for plasticized PVC membranes. Although the diffusion coefficients of the SR and plasticized PVC membranes were similar, the SR membranes had the lowest water uptake of all membranes. This can be beneficial in preventing the formation of detrimental water layers in all-solid-state ion-selective electrodes. With FTIR-ATR, one can monitor the accumulation of different forms of water, i.e., monomeric, dimeric, clustered, and bulk water. PMID:19527006

  8. Fluorescence, CD, attenuated total reflectance (ATR) FTIR, and sup 13 C NMR characterization of the structure and dynamics of synthetic melittin and melittin analogues in lipid environments

    Energy Technology Data Exchange (ETDEWEB)

    Weaver, A.J.; Prendergast, F.G. (Mayo Foundation, Rochester, MN (United States)); Kemple, M.D. (Indiana Univ.-Purdue Univ., Indianapolis (United States)); Brauner, J.W.; Mendelsohn, R. (Rutgers, The State Univ. of New Jersey, Newark (United States))

    1992-02-11

    The structure and dynamics of synthetic melittin (MLT) and MLT analogues bound to monomyristoylphosphatidylcholine micelles, dimyristoylphosphatidylcholine vesicles, and diacylphosphatidylcholine films have been investigated by fluorescence, CD, attenuated total reflectance (ATR) FTIR, and {sup 13}C NMR spectroscopy. All of these methods provide information about peptide secondary structure and/or about the environment of the single tryptophan side chain in these lipid environments. ATR-FTIR data provide additional information about the orientation of helical peptide segments with respect to the bilayer plane. Steady-state fluorescence anisotropy, fluorescence lifetime, and {sup 13}C NMR relaxation data are used in concert to provide quantitative information about the dynamics of a single {sup 13}C{alpha}-labeled glycine incorporated into each of the MLT peptides at position 12. The cumulative structural and dynamic data are consistent with a model wherein the N-terminal {alpha}-helical segment of these peptides is oriented perpendicular to the bilayer plane. Correlation times for the lysolipid-peptide complexes provide evidence for binding of a single peptide monomer per micelle. A model for the membranolytic action of MLT and MLT-like peptides is proposed.

  9. ATR-FTIR Based Pre and Post Formulation Compatibility Studies for the Design of Niosomal Drug Delivery System Containing Nonionic Amphiphiles and Chondroprotective Drug

    International Nuclear Information System (INIS)

    Pharmaceutical compatibility studies are considered as the most important and first screening stage during development of pharmaceutical drug product. Attenuated total reflectance/fourier transform infrared (ATR-FTIR) is one of the techniques currently available to pharmaceutical scientists for investigating the compatibilities between active drug and inactive pharmaceutical ingredients. The present study was designed to assess the interaction among different niosomes forming components i.e nonionic amphiphiles and chondroprotective/antiinflamatory drug Diacerein by ATR-FTIR method. Physical mixtures and niosomes were prepared by physical mixing and thin film hydration method, respectively. The individual niosomal components, physical mixtures as well as niosomal formulations were analyzed. The spectra of Diacerein showed characteristic peaks at 3300 cm/sup -1/(-COOH) and 760 cm/sup -1/(msubstituted benzene), Span 60 at 2916 cm/sup -1/(-OH), Span 80 at 1740 cm/sup -1/(5- membered ring), Span 85 at 1643 cm/sup -1/(ketone with 5-membered ring), Tween 20 at 1734 cm/sup 1/ (5-membered ring) and Tween 80 at 3488 cm/sup -1/(-OH). The characteristic peaks of Diacerein were present in niosomal formulations with slight shift at 3355-3379 cm/sup -1/(-COOH) and 760-770 cm/sup -1/(m-substituted benzene). This work suggested no significant interaction in characteristic peaks of Diacerein after combining with nonionic surfactants as physical mixtures and niosomal formulations which proposed potential for niosomes to encapsulate diacerein in their micro vicinity. (author)

  10. MRN- and 9-1-1-Independent Activation of the ATR-Chk1 Pathway during the Induction of the Virulence Program in the Phytopathogen Ustilago maydis.

    Science.gov (United States)

    Tenorio-Gómez, María; de Sena-Tomás, Carmen; Pérez-Martín, Jose

    2015-01-01

    DNA damage response (DDR) leads to DNA repair, and depending on the extent of the damage, to further events, including cell death. Evidence suggests that cell differentiation may also be a consequence of the DDR. During the formation of the infective hypha in the phytopathogenic fungus Ustilago maydis, two DDR kinases, Atr1 and Chk1, are required to induce a G2 cell cycle arrest, which in turn is essential to display the virulence program. However, the triggering factor of DDR in this process has remained elusive. In this report we provide data suggesting that no DNA damage is associated with the activation of the DDR during the formation of the infective filament in U. maydis. We have analyzed bulk DNA replication during the formation of the infective filament, and we found no signs of impaired DNA replication. Furthermore, using RPA-GFP fusion as a surrogate marker of the presence of DNA damage, we were unable to detect any sign of DNA damage at the cellular level. In addition, neither MRN nor 9-1-1 complexes, both instrumental to transmit the DNA damage signal, are required for the induction of the above mentioned cell cycle arrest, as well as for virulence. In contrast, we have found that the claspin-like protein Mrc1, which in other systems serves as scaffold for Atr1 and Chk1, was required for both processes. We discuss possible alternative ways to trigger the DDR, independent of DNA damage, in U. maydis during virulence program activation.

  11. Kaposi sarcoma herpes virus latency associated nuclear antigen protein release the G2/M cell cycle blocks by modulating ATM/ATR mediated checkpoint pathway.

    Directory of Open Access Journals (Sweden)

    Amit Kumar

    Full Text Available The Kaposi's sarcoma-associated herpesvirus infects the human population and maintains latency stage of viral life cycle in a variety of cell types including cells of epithelial, mesenchymal and endothelial origin. The establishment of latent infection by KSHV requires the expression of an unique repertoire of genes among which latency associated nuclear antigen (LANA plays a critical role in the replication of the viral genome. LANA regulates the transcription of a number of viral and cellular genes essential for the survival of the virus in the host cell. The present study demonstrates the disruption of the host G2/M cell cycle checkpoint regulation as an associated function of LANA. DNA profile of LANA expressing human B-cells demonstrated the ability of this nuclear antigen in relieving the drug (Nocodazole induced G2/M checkpoint arrest. Caffeine suppressed nocodazole induced G2/M arrest indicating involvement of the ATM/ATR. Notably, we have also shown the direct interaction of LANA with Chk2, the ATM/ATR signalling effector and is responsible for the release of the G2/M cell cycle block.

  12. Critical coupling layer thickness for positive or negative Goos-Hänchen shifts near the excitation of backward surface polaritons in Otto-ATR systems

    Science.gov (United States)

    Zeller, Mariana A.; Cuevas, Mauro; Depine, Ricardo A.

    2015-05-01

    We present a theoretical analysis of the lateral displacement (Goos-Hänchen shift) of spatially limited beams reflected from attenuated total reflection (ATR) devices in the Otto configuration when backward surface plasmon polaritons are excited at the interface between a positive refractive index slab and a semi-infinite metamaterial with a negative refractive index. First, the stationary phase approximation and a phenomenological model based on the properties of the complex poles and zeroes of the reflection coefficient are used to demonstrate that: (i) the excitation of backward surface waves can lead to both negative and positive (and not exclusively negative) Goos-Hänchen shifts, and (ii) the sign of the shift depends on whether the value of the coupling layer thickness is higher or lower than a critical value characteristic of the ATR structure. Then, these findings are verified through rigorous calculations of the spatial structure of the reflected beam. For incident beams with a Gaussian profile, the lateral shift calculated as the first moment of the field distribution of the reflected beam agrees quite well with the predictions of approximate analysis. Near the resonant excitation of the backward surface plasmon polariton, large (negative or positive) Goos-Hänchen shifts are obtained, along with a splitting of the reflected beam.

  13. ATR-SEIRAS study of CO adsorption and oxidation on Rh modified Au(111-25 nm) film electrodes in 0.1 M H2SO4

    International Nuclear Information System (INIS)

    Rh modified Au(111-25 nm) electrodes, prepared by electron beam evaporation and galvanostatic deposition, were employed to study adsorption and electro-oxidation of CO on Rh in 0.1 M sulfuric acid solution by in situ attenuated total reflection surface enhanced infrared absorption spectroscopy (ATR-SEIRAS). The results of ATR-SEIRAS experiments were compared with those obtained by infrared reflection absorption spectroscopy on three low-index Rh single crystal surfaces. The Rh film deposited on Au(111-25 nm) electrode consists of 3D clusters forming a highly stepped [n(111) × (111)]-like surface with narrow (111) terraces. When CO was dosed at the hydrogen adsorption potential region, CO adsorbed in both atop (COL) and bridge (COB) configurations, as well as coadsorbed water species, were detected on the Rh film electrode. A partial interconversion of spectroscopic bands due to the CO displacement from bridge to atop sites was found during the anodic potential scan, revealing that there is a potential-dependent preference of CO adsorption sites on Rh surfaces. Our data indicate that CO oxidation on Rh electrode surface in acidic media involves coadsorbed water and follows the nucleation and growth model of a Langmuir-Hinshelwood type reaction

  14. ATR-FTIR and XPS study on the structure of complexes formed upon the adsorption of simple organic acids on aluminum hydroxide

    Institute of Scientific and Technical Information of China (English)

    GUAN Xiao-hong; CHEN Guang-hao; SHANG Chii

    2007-01-01

    Information on the binding of organic ligands to metal (hydr)oxide surfaces is useful for understanding the adsorption behaviour of natural organic matter (NOM) on metal (hydr)oxide. In this study, benzoate and salicylate were employed as the model organic ligands and aluminum hydroxide as the metal hydroxide. The attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectra revealed that the ligands benzoate and salicylate do coordinate directly with the surface of hydrous aluminum hydroxide, thereby forming inner-sphere surface complexes. It is concluded that when the initial pH is acidic or neutral, monodentate and bridging complexes are to be formed between benzoate and aluminum hydroxide while bridging complexes predominate when the initial pH is alkalic. Monodentate and bridging complexes can be formed at pH 5 while precipitate and bridging complexes are formed at pH 7 when salicylate anions are adsorbed on aluminum hydroxide. The X-ray photoelectron (XP) spectra demonstrated the variation of C 1s binding energy in the salicyate and phenolic groups before and after adsorption. It implied that the benzoate ligands are adsorbed through the complexation between carboxylate moieties and the aluminum hydroxide surface, while both carboxylate group and phenolic group are involved in the complexation reaction when salicylate is adsorbed onto aluminum hydroxide. The information offered by the XPS confirmed the findings obtained with ATR-FTIR.

  15. A novel technique for the rapid determination of tributyl phosphate degradation from alkaline hydrolysis in aqueous and organic phases using FTIR-ATR and verification of this technique by gas chromatography

    International Nuclear Information System (INIS)

    This paper details a semi-quantitative method for determining tributyl phosphate (TBP) degradation from alkaline hydrolysis using FTIR-ATR accompanied by GC verification and it provides a method to extract TBP from aqueous media associated with its use in nuclear reprocessing. The amount of TBP determined by GC decreases from 95 to 36 % after approximately 4 1/2 h in the reaction. TBP present in the organic phase predicted by the FTIR-ATR technique shows that TBP decreases from 97 to 42 %. Up to 15 % of TBP and 40 % of DBP were extracted from the precipitate based on the extraction recoveries determined. (author)

  16. Model Persediaan Terintegrasi Berdasarkan Perbandingan Biaya

    OpenAIRE

    Sibarani, Johannes Andre

    2011-01-01

    Every company must be has the policy in managing its inventory, so that production processes can be run well. Two mathematical model of inventories are analised. The aim of these models are minimizing the total cost of inventories on a company and its distributor. The first mathematical model is IDQ (Identical Delivery Quantity) model, the amount of delivery to distributor is identically for every replenishment. The second one is DWP (Delivery What is Produced) model, the amoun...

  17. Penciptaan Naskah Drama Narcissus Berdasarkan Mitologi Yunani

    Directory of Open Access Journals (Sweden)

    Titis Rahayuningtiar

    2013-11-01

    Full Text Available Naskah drama Narcissus adalah sebuah naskah drama dengan genre klasik yang mengusung irama tragis. Narcissus adalah sebuah tokoh yang berasal dari cerita mitologi Yunani. Narcissus merupakan seorang pemuda sombong yang sangat mengagungkan keindahan yang ada dalam dirinya. Hingga suatu hari Narcissus dikutuk untuk jatuh cinta pada bayangannya sendiri. Penciptaan naskah drama Narcissus bertujuan untuk mengisi kelangkaan naskah drama yang sumber idenya berasal dari fenomena narsis di masyarakat dan konsep irama tragis dalam mitologi Yunani. Metode penciptaan mengusung sebuah metode kreatif yang terdiri dari tahap eksplorasi, pembentukan dan improvisasi. Hasil  penciptaan naskah ini adalah sebuah naskah bergenre klasik yang memiliki pesan moral bahwasanya seseorang yang menganggap dirinya sempurna akan memberi akibat buruk untuk dirinya sendiri. Kesempurnaan hanyalah milik Tuhan.Kata kunci: Narcissus, Yunani, mitologi.ABSTRACTThe Creation of the Drama Script of Narcissus Based on the Greek Mythology. The script of Narcissus drama is a script of drama with a classical genre which carries the tragic rhythm on it. Narcissus is a character in the story of the Greek Mythology. He is an arrogant young man who really likes to glorify himself on the beauty of what he has. Unfortunatelly, one day he was cursed to fall in love with his own reflection. The creation of drama script of Narcissus is aimed to fill the scarcity of drama scripts in which the basic sources of idea come from narcissistic phenomena in a society and the concept of the tragic rhythm in the Greek mythology. The method of creation brings a creative method comprising the steps of exploration, creation, and improvisation. The result of this script creation is a classical genre script which has a moral message in which a person who considers himself perfectly will give a bad impact to himself. Nevertheles, perfection belongs to God only.Key words: Narcissius, Greek , mythology

  18. KEKERABATAN Hoya (ASCLEPIADACEAE SUMATERA BERDASARKAN KARAKTER MORFOLOGI

    Directory of Open Access Journals (Sweden)

    Sri Rahayu

    2011-01-01

    Full Text Available Morphological relationships analysis of Hoya species from Sumatra was carried out. The study was taken both from the living collections at Bogor Botanic Garden and the herbarium specimens at Herbarium Bogoriense. There are 25 Hoya species in Sumatra resulted from the total of 129 samples number. Cluster analysis was run by Clique of Phylip software based on morphological diagnostic characters. Five paraphiletic groups were resulted from the phylogenic analyses. The groups constructed from this analysis were tending to be identical with the Schlecter's section classification.

  19. PERUMUSAN STRATEGI PERUSAHAAN BERDASARKAN COMPETITIVE ADVANTAGE

    Directory of Open Access Journals (Sweden)

    Endah Utami

    2012-12-01

    Full Text Available Perusahaan Tenun Ikat Lestari Indah adalah perusahaan pembuatan kain dengan alat tenun bukan mesin. Dalam menjalankan bisnisnya, perusahaan menghadapi berbagai kendala, diantaranya daya beli masyarakat menurun akibat situasi perekonomian yang tidak menentu serta persaingan harga yang tidak sehat antar pengrajin. Tujuan penelitian ini adalah untuk merumuskan strategi perusahaan sesuai dengan kondisi internal dan eksternal perusahaan. Metode yang digunakan dalam penelitian ini adalah analisis SWOT, I-E (Internal-External, dan QSPM (Quantitative Strategy Planning Matrix. Hasil penelitian menunjukkan posisi bisnis perusahaan berada pada posisi pertumbuhan, sehingga strategi yang dirumuskan adalah menghindari kehilangan profit dan penjualan. Dari hasil analisis QSPM (Quantitative Strategy Planning Matrix diketahui strategi yang terpilih untuk strategi jangka pendek adalah menjalin hubungan baik dengan konsumen tetap , strategi jangka pendek-menengah adalah menjalin kerjasama dengan biro-biro perjalanan wisata, strategi jangka menengah adalah menetapkan tingkatan harga sesuai kualitas dan desain, dan strategi jangka panjang adalah memperluas jaringan pemasaran di dalam dan luar negeri.

  20. Alternatif Pengembangan Pendidikan Berdasarkan Nilai Kebutuhan Khusus

    Directory of Open Access Journals (Sweden)

    Silverius Constantino Johanes Maria Lake

    2015-06-01

    Full Text Available The Community College is a kind of higher education. It focuses on diploma degree (the Two Year College. The objectives of Community College are to educate and to train students for getting working skills for jobs. The Community College has competencies that are suitable with the working area and the local needs. To run the Community College goodly and truly, values is needed. Those values influence the mission of Community College. The question was why Indonesian Higher Education accepts the Community College as a model of higher education. The problem was related to some models of higher education that do not fulfill the demands of companies yet. Therefore the Community College becomes a choice to answer the demands of industries and etrepreneurship. The right references to learn about Community College are American Community Colleges such as Highline Community College (Seattle, WA and Kapiolani Community Collere (Honolulu, HI. Each of those Community Colleges has the best program for example Highline Community College in Business Management, then Kapiolani Community College in Culinary Arts. This article used descriptive and argumentative method to show the importance of Commuity College in Indonesia.

  1. Semantik Suksesi Sistem Informasi Berdasarkan Populasi Teks

    OpenAIRE

    Elfida, Maria

    2015-01-01

    The information system success relates to the measurement of user satisfaction and information system actuators. In other words, the existence of information systems are always related to organizations or groups of people that involve information technology for processing and presenting data in decision making. Therefore, the existence of an information system deal with information technology changes, changes in demanding service to users of information systems. Especially the ...

  2. Alternatif Pengembangan Pendidikan Berdasarkan Nilai Kebutuhan Khusus

    OpenAIRE

    Silverius Constantino Johanes Maria Lake

    2015-01-01

    The Community College is a kind of higher education. It focuses on diploma degree (the Two Year College). The objectives of Community College are to educate and to train students for getting working skills for jobs. The Community College has competencies that are suitable with the working area and the local needs. To run the Community College goodly and truly, values is needed. Those values influence the mission of Community College. The question was why Indonesian Higher Education accepts th...

  3. Garis dan Titik Berdasarkan Riset Visual

    OpenAIRE

    Devi Kurniawati Homan

    2015-01-01

    A design may not be created instantly, without going through a process of creation. At the beginning it all starts from nothing, blank. It requires a visual research to make a visual communication. Without a visual research, the design will not have a strong meaning to be communicated. When an idea is created, the next step is to generate the idea to become a design. The first thing a designer to do is drawing a line. Line is one of the design basic elements. In the journey to make a good des...

  4. Garis dan Titik Berdasarkan Riset Visual

    Directory of Open Access Journals (Sweden)

    Devi Kurniawati Homan

    2015-06-01

    Full Text Available A design may not be created instantly, without going through a process of creation. At the beginning it all starts from nothing, blank. It requires a visual research to make a visual communication. Without a visual research, the design will not have a strong meaning to be communicated. When an idea is created, the next step is to generate the idea to become a design. The first thing a designer to do is drawing a line. Line is one of the design basic elements. In the journey to make a good design, a designer requires a visual record to assist her/him in remembering what is important. A visual record contains not only visual things seen by eyes, but also the perceived things. What is felt at the moment is important because it is easier to forget than visual memory. Sense is an important element in design. A good work will easily be communicated not only by visuals but also from senses. The study was conducted as an insight into the process of making a visual record of nirmana using visual recording method in the exploration of dots and dashes. This method was used to sharpen the sensitivity of students to dots and lines. Initially, the students visited a traditional market to do the visual research. After that, they created a visual record in the form of the composition of dots and lines in nirmana to remember the taste, smell, and visual experiences.

  5. Indikasi munculnya Kubah Lava berdasarkan Rekaman Seismik

    Directory of Open Access Journals (Sweden)

    S. R. Wittiri

    2014-06-01

    Full Text Available http://dx.doi.org/10.17014/ijog.vol4no2.20092In the last two decades, there are six volcanoes erupting and are ended up with the growth of lava dome at the crater. Among them, formerly there are three volcanoes that have crater lakes. For the intermediate magma, like most Indonesian volcanoes, the lava dome formation is a usual phenomenon. The interesting symptom is indicated by the seismic waves. They are supposed to relate to the magma breakthrough into the surface. The seismic phenomena of those volcanoes have a similarity, which can be estimated that the  mechanism of rock fracturing is relatively similar.  

  6. PENATAAN RUANG LAUT BERDASARKAN INTEGRATED COASTAL MANAGEMENT

    Directory of Open Access Journals (Sweden)

    Dina Sunyowati

    2008-10-01

    Full Text Available The planning of coastal spatial arrangement must be put in the valid spatial planning system. Law Number 26 of 2007 on Spatial Planning and it is in fact related with land spatial planning, although that ocean and air spatial management will be arranged in separate law. The legal for coastal zone management is determined by using the principles of integrated coastal management by focusing on area or zone authority system. The integrated of coastal zones management regulations should be followed by the planning of coastal spatial arrange­ment. Therefore, certain synchronization at coastal zones governance is very important issue since by integrating and coordinating other related regulations and therefore conflict of norm can be minimized in the spatial planning coastal zone.

  7. Faktor Risiko Penyakit Ginjal Kronik Berdasarkan Analisis Cross-sectional Data Awal Studi Kohort Penyakit Tidak Menular Penduduk Usia 25-65 Tahun di Kelurahan Kebon Kalapa, Kota Bogor Tahun 2011

    Directory of Open Access Journals (Sweden)

    Eva Sulistiowati

    2015-11-01

    Full Text Available AbstractGlomerular Filtration Rate (GFR is associated with renal function and used to diagnose Chronic Kidney Disease (CKD. CKD is considered a serious worldwide public health problem, and the prevalence is increasing dramatically. The aim of the analisis is to explore of the factors associated with estimated Glomerular Filtration Rate (eGFR. This analysis used subset baseline data Cohort Study Non-Communicable Diseases (NCD 2011 with a cross-sectional design. CKD was defined as those withan estimated glomerular filtration rate (eGFR <60 mL/min/1.73 m2. We analyzed of 1932 subjects (820 males and 1112 females aged 25-65 years old. GFR was estimated by using calibrated serum creatinine level with a formula CKD-epi, devided into ≥60 mL/min/1.73 m2 and <60 mL/min/1.73 m2. Subject with e-GFR <60 mL/min/1.73 m2 was 2,3%, and increased remarkably with age. Multivariate logistic regression analysis demonstrated that age of 49-65 years (OR=13.57; 95% CI: 4.73-38.97,economic status quintile 1 (OR=4.44; 95% CI: 1.14-17.39, hipertension (OR=3.71;95% CI: 1.82-7.59, male gender (OR=2.97; 95% CI: 1.49-5.92, diabetes mellitus (OR=2.54; 95% CI=1.24-5.20, obesity(OR=2.51; 95% CI: 1.20-5.25, were significant factors that were independently associated with CKD.Keywords : risk factors; glomerular filtration rate; CKDAbstrakLaju filtrasi glomerulus (LFG berhubungan dengan kondisi fungsi ginjal dan digunakan sebagai penentu diagnosis Penyakit Ginjal Kronik (PGK. PGK merupakan masalah kesehatan yang serius dan prevalensinya meningkat secara drastis. Tujuan analisis ini untuk mengetahui faktor yang berhubungan dengan PGK. Data diambil dari subset data Studi Kohor Penyakit Tidak Menular (PTM di Kota Bogor Tahun 2011, menggunakan disain analisis potong lintang. Subjek yang dianalis berjumlah 1932 orang(1112 perempuan dan 820 laki-laki berumur 25-65 tahun. LFG diklasifikasikan menurut estimasi LFG (e-LFG berdasarkan kriteria CKD-epi, dengan kategori ≥60 mL/min/1,73 m2

  8. Tratamiento del maxilar posterior atrófico mediante técnica de reconstrucción tridimensional con elevación de seno y abordaje «en tunel»

    Directory of Open Access Journals (Sweden)

    Andrés Restoy

    2015-01-01

    Conclusiones: El tratamiento del sector posterior maxilar atrófico mediante reconstrucción tridimensional con autoinjerto óseo, elevación sinusal y abordaje por tunelización es una técnica que proporciona resultados predecibles y estables, permitiendo la rehabilitación con coronas sobre implantes dentales de dimensiones adecuadas.

  9. Selective Alcohol Oxidation by a Copper TEMPO Catalyst: Mechanistic Insights by Simultaneously Coupled Operando EPR/UV-Vis/ATR-IR Spectroscopy.

    Science.gov (United States)

    Rabeah, Jabor; Bentrup, Ursula; Stößer, Reinhard; Brückner, Angelika

    2015-09-28

    The first coupled operando EPR/UV-Vis/ATR-IR spectroscopy setup for mechanistic studies of gas-liquid phase reactions is presented and exemplarily applied to the well-known copper/TEMPO-catalyzed (TEMPO=(2,2,6,6-tetramethylpiperidin-1-yl)oxyl) oxidation of benzyl alcohol. In contrast to previous proposals, no direct redox reaction between TEMPO and Cu(I) /Cu(II) has been detected. Instead, the role of TEMPO is postulated to be the stabilization of a (bpy)(NMI)Cu(II) -O2 (⋅-) -TEMPO (bpy=2,2'-bipyridine, NMI=N-methylimidazole) intermediate formed by electron transfer from Cu(I) to molecular O2 . PMID:26174141

  10. New organic single crystal of (benzylthio)acetic acid: Synthesis, crystal structure, spectroscopic (ATR-FTIR, 1H and 13C NMR) and thermal characterization

    Science.gov (United States)

    Sienkiewicz-Gromiuk, Justyna; Tarasiuk, Bogdan; Mazur, Liliana

    2016-04-01

    (Benzylthio)acetic acid (Hbta) was synthesized with 78% yield from benzyl chloride and thiourea as substrates. Well-shaped crystals of Hbta were grown by slow solvent evaporation technique from pure methanol. The compound was investigated by single-crystal X-ray and powder diffraction techniques and was also characterized by other analytical methods, like ATR-FTIR, 1H and 13C NMR and TG/DSC. The acid molecule adopts bent conformation in the solid state. The crystal structure of Hbta is stabilized by numerous intermolecular interactions, including O-H···O, C-H···O, C-H···S and C-H···π contacts. Thermal decomposition of the obtained material takes place above 150 °C.

  11. El cuento de la verdad "El cuento de la verdad "El cuento de atrás" y "El lápiz del carpintero"

    OpenAIRE

    Bazán Rodríguez, Óscar

    2012-01-01

    Uno de los puntos claves que se ha venido trabajando en la literatura española contemporánea es el tratamiento de la memoria, y dentro de este tema, cómo se afronta un concepto tan inasible como el del “la verdad” desde un punto de vista narrativo. En este artículo nos aproximamos a la visión de “la verdad” a través de dos de las novelas más importantes que tocan la Guerra Civil española: “El cuarto de atrás”, de Carmen Martín Gaite; y “El lápiz del carpintero”, de Manuel Rivas...

  12. An Introduction to the Chinese Speech Recognition Front-End of the NICT/ATR Multi-Lingual Speech Translation System

    Institute of Scientific and Technical Information of China (English)

    ZHANG Jinsong; Takatoshi Jitsuhiro; Hirofumi Yamamoto; HU Xinhui; Satoshi Nakamura

    2008-01-01

    This paper introduces several important features of the Chinese large vocabulary continuous speech recognition system in the NICT/ATR multi-lingual speech-to-speech translation system.The features include: (1) a flexible way to derive an information rich phoneme set based on mutual information between a text corpus and its phoneme set; (2) a hidden Markov network acoustic model and a successive state split-ting algorithm to generate its model topology based on a minimum description length criterion; and (3) ad-vanced language modeling using multi-class composite N-grams.These features allow a recognition per-formance of 90% character accuracy in tourism related dialogue with a real time response speed.

  13. Study of Barley Grain Molecular Structure for Ruminants Using DRIFT, FTIR-ATR and Synchrotron Radiation Infrared Microspectroscopy (SR-IMS): A Review

    Science.gov (United States)

    Yu, Peiqiang

    2012-05-01

    Barley inherent structures are highly associated with nutrient utilization and availability in both humans and animals. Barley has different degradation kinetics compared with other cereal grains. It has a relatively higher degradation rate and extent, which often cause digestive disorder in the rumen. Therefore understanding barley inherent structure at cellular and molecular levels and processing-induced structure changes is important, because we can manipulate barley inherent structures and digestive behaviors. Several molecular spectroscopy techniques can be used to detect barley inherent structures at cellular and molecular levels. This article reviews several applications of the IR molecular spectral bioanalytical techniques - DRIFT, FT/IR-ATR and SR-IMS for barley chemistry, molecular structure and molecular nutrition research

  14. FTIR-ATR study of the influence of the pyrimidine analog of fluphenazine on the chain-melting phase transition of sphingomyelin membranes

    Science.gov (United States)

    Kuć, Marta; Cieślik-Boczula, Katarzyna; Świątek, Piotr; Jaszczyszyn, Agata; Gąsiorowski, Kazimierz; Malinka, Wiesław

    2015-09-01

    The membrane perturbing potency of the highly effective anti-multidrug resistance (MDR) pyrimidine analog of fluphenazine (FPh-prm), has been studied using attenuated total reflectance Fourier-transfer infrared spectroscopy (FTIR-ATR). The temperature- and FPh-prm-dose-dependent evolutions of the infrared spectra of FPh-prm/sphingomyelin (SM) mixtures were analyzed using principal component analysis (PCA). It has been postulated that the distinct anti-MDR activity of FPh-prm could be related to its ability to affect the modification of SM membranes. A reduction in the temperature of the chain-melting phase transition was observed in FPh-prm-mixed SM membranes together with the loosing of the phase transition cooperativity. Increasing the temperature led to the trans to gauche isomerization of FPh-prm-rich lipid membranes, which resulted in the gradual release of FPh-prm from the lipid membrane to the water phase.

  15. Fourier transform infrared-attenuated total reflectance (FTIR-ATR spectroscopy and chemometric techniques for the determination of adulteration in petrodiesel/biodiesel blends

    Directory of Open Access Journals (Sweden)

    Armando Guerrero Peña

    2014-06-01

    Full Text Available We propose an analytical method based on fourier transform infrared-attenuated total reflectance (FTIR-ATR spectroscopy to detect the adulteration of petrodiesel and petrodiesel/palm biodiesel blends with African crude palm oil. The infrared spectral fingerprints from the sample analysis were used to perform principal components analysis (PCA and to construct a prediction model using partial least squares (PLS regression. The PCA results separated the samples into three groups, allowing identification of those subjected to adulteration with palm oil. The obtained model shows a good predictive capacity for determining the concentration of palm oil in petrodiesel/biodiesel blends. Advantages of the proposed method include cost-effectiveness and speed; it is also environmentally friendly.

  16. Corrosion protection of galvanized steel and electroplating steel by decanoic acid in aqueous solution: Electrochemical impedance spectroscopy, XPS and ATR-FTIR

    Energy Technology Data Exchange (ETDEWEB)

    Lebrini, M. [Laboratoire des Procedes d' Elaboration des Revetements Fonctionnels, LSPES UMR CNRS 8008, ENSCL, BP 90108, F-59652 Villeneuve d' Ascq Cedex (France)], E-mail: mounim.lebrini@pop.ensc-lille.fr; Fontaine, G. [Laboratoire des Procedes d' Elaboration des Revetements Fonctionnels, LSPES UMR CNRS 8008, ENSCL, BP 90108, F-59652 Villeneuve d' Ascq Cedex (France); Gengembre, L. [Unite de Catalyse et Chimie du solide UMR 8181 Bat C3, USTL, F-59655 Villeneuve d' Ascq Cedex (France); Traisnel, M. [Laboratoire des Procedes d' Elaboration des Revetements Fonctionnels, LSPES UMR CNRS 8008, ENSCL, BP 90108, F-59652 Villeneuve d' Ascq Cedex (France); Lerasle, O.; Genet, N. [TOTAL France, Centre de Recherche de Solaize, Chemin du canal, BP 22, F-69360 Solaize (France)

    2009-06-15

    The inhibiting action of decanoic acid towards the corrosion behaviour of galvanized steel and electroplating steel in aqueous solution has been studied using electrochemical impedance spectroscopy (EIS) techniques. Data obtained from EIS show a frequency distribution and therefore a modelling element with frequency dispersion behaviour, a constant phase element (CPE) has been used. Results obtained revealed that decanoic acid is an effective inhibitor. The better performance was obtained in the case of electroplating steel. X-ray photoelectron spectroscopy surface analysis shows that, decanoic acid is chemisorbed on surface of galvanized steel and electroplating steel. These studies have shown that the active site for binding the film on metal surface is the anionic carboxylate head. Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy was used to identify the nature of the deposits on the metal surface.

  17. Técnicas FT-IR (PAS, UATR e Objetiva ATR Aplicadas à Caracterização de EPDM Modificada com Plasma

    Directory of Open Access Journals (Sweden)

    Renata Patrícia dos Santos

    2014-06-01

    Full Text Available Técnicas FT-IR (UATR, PAS e MIC, com objetiva ATR foram escolhidas para a caracterização da superfície da borracha de EPDM vulcanizada, após tratamento em plasma de Ar/O2 e N2/H2/Ar gerado em micro-ondas. Após tratamento, grupos oxigenados foram detectados por UATR e MIC/FT-IR, com objetiva ATR para as duas misturas gasosas, e possíveis grupos nitrogenados foram inseridos na superfície das amostras tratadas com plasma de N2/H2/Ar. A análise MIC/FT-IR sugeriu a formação de grupos em uma camada mais externa, para as amostras tratadas com plasma de N2/H2/Ar, sendo possível observar a redução na intensidade das bandas da absorção do CH2 e CH3, o que pode estar relacionado ao fato de o nitrogênio ser um gás menos permeável que o oxigênio. Diferentes velocidades, 0,05 cm.s-1 e 0,2 cm.s-1, foram utilizadas na análise PAS para avaliar a superfície após tratamento, e apenas para a velocidade 0,05 cm.s-1 com plasma de Ar/O2 alterações espectroscópicas foram detectadas. A redução na medida de ângulo de contato e o aumento na resistência ao descascamento da colagem indicaram melhoras nas propriedades adesivas da superfície. Falhas de adesão foram observadas entre a interface do filme de adesivo de PU e da borracha de EPDM, e confirmados por UATR/FT-IR.

  18. New insights into the mechanism of interaction between CO2 and polymers from thermodynamic parameters obtained by in situ ATR-FTIR spectroscopy.

    Science.gov (United States)

    Gabrienko, Anton A; Ewing, Andrew V; Chibiryaev, Andrey M; Agafontsev, Alexander M; Dubkov, Konstantin A; Kazarian, Sergei G

    2016-03-01

    This work reports new physical insights of the thermodynamic parameters and mechanisms of possible interactions occurring in polymers subjected to high-pressure CO2. ATR-FTIR spectroscopy has been used in situ to determine the thermodynamic parameters of the intermolecular interactions between CO2 and different functional groups of the polymers capable of specific interactions with sorbed CO2 molecules. Based on the measured ATR-FTIR spectra of the polymer samples subjected to high-pressure CO2 (30 bar) at different temperatures (300-340 K), it was possible to characterize polymer-polymer and CO2-polymer interactions. Particularly, the enthalpy and entropy of the formation of the specific non-covalent complexes between CO2 and the hydroxy (-OH), carbonyl (C[double bond, length as m-dash]O) and hydroxyimino ([double bond, length as m-dash]N-OH) functional groups of the polymer samples have been measured. Furthermore, the obtained spectroscopic results have provided an opportunity for the structure of these complexes to be proposed. An interesting phenomenon regarding the behavior of CO2/polymer systems has also been observed. It has been found that only for the polyketone, the value of enthalpy was negative indicating an exothermic process during the formation of the CO2-polymer non-covalent complexes. Conversely, for the polyoxime and polyalcohol samples there is a positive enthalpy determined. This is a result of the initial polymer-polymer interactions requiring more energy to break than is released during the formation of the CO2-polymer complex. The effect of increasing temperature to facilitate the breaking of the polymer-polymer interactions has also been observed. Hence, a mechanism for the formation of CO2-polymer complexes was suggested based on these results, which occurs via a two-step process: (1) the breaking of the existing polymer-polymer interactions followed by (2) the formation of new CO2-polymer non-covalent interactions.

  19. Proposal of AAA-battery-size one-shot ATR Fourier spectroscopic imager for on-site analysis: Simultaneous measurement of multi-components with high accuracy

    Science.gov (United States)

    Hosono, Satsuki; Qi, Wei; Sato, Shun; Suzuki, Yo; Fujiwara, Masaru; Hiramatsu, Hiroyuki; Suzuki, Satoru; Abeygunawardhana, P. K. W.; Wada, Kenji; Nishiyama, Akira; Ishimaru, Ichiro

    2015-03-01

    For simultaneous measurement of multi-components on-site like factories, the ultra-compact (diameter: 9[mm], length: 45[mm], weight: 200[g]) one-shot ATR (Attenuated Total Reflection) Fourier spectroscopic imager was proposed. Because the proposed one-shot Fourier spectroscopic imaging is based on spatial-phase-shift interferometer, interferograms could be obtained with simple optical configurations. We introduced the transmission-type relativeinclined phase-shifter, that was constructed with a cuboid prism and a wedge prism, onto the optical Fourier transform plane of infinity corrected optical systems. And also, small light-sources and cameras in the mid-infrared light region, whose size are several millimeter on a side, are essential components for the ultra-compact spectroscopic configuration. We selected the Graphite light source (light source area: 1.7×1.7[mm], maker: Hawkeye technologies) whose radiation factor was high. Fortunately, in these days we could apply the cost-effective 2-dimensional light receiving device for smartphone (e.g. product name: LEPTON, maker: FLIR, price: around 400USD). In the case of alcoholic drinks factory, conventionally workers measure glucose and ethanol concentrations by bringing liquid solution back to laboratories every day. The high portable spectroscopy will make it possible to measure multi-components simultaneously on manufacturing scene. But we found experimentally that absorption spectrum of glucose and water and ethanol were overlapped each other in near infrared light region. But for mid-infrared light region, we could distinguish specific absorption peaks of glucose (@10.5[μm]) and ethanol (@11.5[μm]) independently from water absorption. We obtained standard curve between absorption (@9.6[μm]) and ethanol concentration with high correlation coefficient 0.98 successfully by ATR imaging-type 2-dimensional Fourier spectroscopy (wavelength resolution: 0.057[μm]) with the graphite light source (maker: Hawkeye

  20. MRN- and 9-1-1-Independent Activation of the ATR-Chk1 Pathway during the Induction of the Virulence Program in the Phytopathogen Ustilago maydis.

    Directory of Open Access Journals (Sweden)

    María Tenorio-Gómez

    Full Text Available DNA damage response (DDR leads to DNA repair, and depending on the extent of the damage, to further events, including cell death. Evidence suggests that cell differentiation may also be a consequence of the DDR. During the formation of the infective hypha in the phytopathogenic fungus Ustilago maydis, two DDR kinases, Atr1 and Chk1, are required to induce a G2 cell cycle arrest, which in turn is essential to display the virulence program. However, the triggering factor of DDR in this process has remained elusive. In this report we provide data suggesting that no DNA damage is associated with the activation of the DDR during the formation of the infective filament in U. maydis. We have analyzed bulk DNA replication during the formation of the infective filament, and we found no signs of impaired DNA replication. Furthermore, using RPA-GFP fusion as a surrogate marker of the presence of DNA damage, we were unable to detect any sign of DNA damage at the cellular level. In addition, neither MRN nor 9-1-1 complexes, both instrumental to transmit the DNA damage signal, are required for the induction of the above mentioned cell cycle arrest, as well as for virulence. In contrast, we have found that the claspin-like protein Mrc1, which in other systems serves as scaffold for Atr1 and Chk1, was required for both processes. We discuss possible alternative ways to trigger the DDR, independent of DNA damage, in U. maydis during virulence program activation.

  1. FTIR-ATR-based prediction and modelling of lignin and energy contents reveals independent intra-specific variation of these traits in bioenergy poplars

    Directory of Open Access Journals (Sweden)

    Taylor Gail

    2011-04-01

    Full Text Available Abstract Background There is an increasing demand for renewable resources to replace fossil fuels. However, different applications such as the production of secondary biofuels or combustion for energy production require different wood properties. Therefore, high-throughput methods are needed for rapid screening of wood in large scale samples, e.g., to evaluate the outcome of tree breeding or genetic engineering. In this study, we investigated the intra-specific variability of lignin and energy contents in extractive-free wood of hybrid poplar progenies (Populus trichocarpa × deltoides and tested if the range was sufficient for the development of quantitative prediction models based on Fourier transform infrared spectroscopy (FTIR. Since lignin is a major energy-bearing compound, we expected that the energy content of wood would be positively correlated with the lignin content. Results Lignin contents of extractive-free poplar wood samples determined by the acetyl bromide method ranged from 23.4% to 32.1%, and the calorific values measured with a combustion calorimeter varied from 17260 to 19767 J g-1. For the development of calibration models partial least square regression and cross validation was applied to correlate FTIR spectra determined with an attenuated total reflectance (ATR unit to measured values of lignin or energy contents. The best models with high coefficients of determination (R2 (calibration = 0.91 and 0.90; R2 (cross-validation = 0.81 and 0.79 and low root mean square errors of cross validation (RMSECV = 0.77% and 62 J g-1 for lignin and energy determination, respectively, were obtained after data pre-processing and automatic wavenumber restriction. The calibration models were validated by analyses of independent sets of wood samples yielding R2 = 0.88 and 0.86 for lignin and energy contents, respectively. Conclusions These results show that FTIR-ATR spectroscopy is suitable as a high-throughput method for lignin and energy

  2. Study of the interface solid/solutions containing PEO-PPO block copolymers and asphaltenes by FTIR/ATR; Estudo de solucoes de copolimeros em bloco de PEO-PPO contendo asfaltenos por FTIR/DTA

    Energy Technology Data Exchange (ETDEWEB)

    Aguiar, Janaina I.S.; Neto, Jessica S.G.; Mansur, Claudia R.E. [Universidade Federal do Rio de Janeiro, Instituto de Macromoleculas, Laboratorio de Macromoleculas e Coloides na Industria de Petroleo, Rio de Janeiro, RJ (Brazil)], E-mails: janaina_333@hotmail.com, kinha_dac_dm@hotmail.com; celias@ima.ufrj.br

    2011-07-01

    The formation of water/oil emulsions can cause problems in various stages of production, processing and refining of petroleum. In this study, the technique of Fourier transform infrared spectroscopy (FTIR) using the method of attenuated total reflectance (ATR) was applied to study the solid-solutions of block copolymers based on poly(ethylene oxide)-poly(propylene oxide) (PEO-PPO) interface and its interaction in this interface with asphaltenic fractions of petroleum. The solid is the crystal of the ATR. Initially, we determined the critical micelle concentration values of the copolymers, which were consistent those obtained by a tensiometer. Bottle Test was also performed to correlate the efficiency of PEO-PPO copolymers in the breaking of water/oil emulsions with its adsorption at the interfaces solutions. (author)

  3. Noninvasive detection by ATR and NIR-DR methods for skin-care ionic materials transported into the skin by iontophoresis

    Science.gov (United States)

    Ueda, Toyotoshi; Watanabe, Yukio; Akao, Ken-ichi; Suzuki, Harue

    2003-12-01

    Two analytical methods without damage to the skin were proposed in order to detect and measure the quantity of the medication transported into the skin by the iontophoresis. The infrared attenuated total reflection (ATR) method was proven to be able to evaluate the content of such a substance as sodium all- trans-retinoate or magnesium ℓ-ascorbyl-2-phosphate in the top (horny) layer of epidermis (about 1 μm under the skin surface), using characteristic bands to the above ion. Another method of near-infrared diffusive-reflection (NIR-DR) technique was shown probably to detect it in the dermis (1 mm under the surface), based on the shift of frequency and the change in intensity for the vibrational combination band of water molecules hydrating the ion. The quantity of the above material decreased monotonically in the horny layer for several hours after the treatment, while in the dermis it increased at first and then decreased via the maximum value.

  4. ATR-FTIR spectroscopy study of the photodegradation protective properties of BP-4 and 4HBP in polyvinyl acetate thin films

    Science.gov (United States)

    Bubev, Emil; Georgiev, Anton; Machkova, Maria

    2016-08-01

    In this paper the protective properties of 4HBP (4-hydroxybenzophenone) and BP-4 (5-benzoyl-2-hydroxy-4-methoxybenzenesulfonic acid) in polyvinyl acetate thin films have been studied. UV-irradiation on the films was applied in real humidity and inert atmosphere for 1, 2 and 4 h accelerated weathering. ATR-FTIR and UV-VIS spectroscopies have been used for qualitative and quantitative estimation of photodepletion of the UV-absorbers and photostability of polyvinyl acetate films. Both UV-absorbers have demonstrated good protective properties in inert atmosphere compared to real humidity. The mechanisms of photochemical reactions of 4HBP (4-hydroxybenzophenone) and BP-4 (5-benzoyl-2-hydroxy-4-methoxybenzenesulfonic acid) were attributed to photoenolization of carbonyl and hydroxyl groups. The experimental results demonstrated that 4HBP (4-hydroxybenzophenone) has higher protective properties than BP-4 (5-benzoyl-2-hydroxy-4-methoxybenzenesulfonic acid). The photodegradation mechanisms of polyvinyl acetate films in inert atmosphere and real humidity were assigned based on the Norrish type I and II reactions.

  5. Sample classification for improved performance of PLS models applied to the quality control of deep-frying oils of different botanic origins analyzed using ATR-FTIR spectroscopy.

    Science.gov (United States)

    Kuligowski, Julia; Carrión, David; Quintás, Guillermo; Garrigues, Salvador; de la Guardia, Miguel

    2011-01-01

    The selection of an appropriate calibration set is a critical step in multivariate method development. In this work, the effect of using different calibration sets, based on a previous classification of unknown samples, on the partial least squares (PLS) regression model performance has been discussed. As an example, attenuated total reflection (ATR) mid-infrared spectra of deep-fried vegetable oil samples from three botanical origins (olive, sunflower, and corn oil), with increasing polymerized triacylglyceride (PTG) content induced by a deep-frying process were employed. The use of a one-class-classifier partial least squares-discriminant analysis (PLS-DA) and a rooted binary directed acyclic graph tree provided accurate oil classification. Oil samples fried without foodstuff could be classified correctly, independent of their PTG content. However, class separation of oil samples fried with foodstuff, was less evident. The combined use of double-cross model validation with permutation testing was used to validate the obtained PLS-DA classification models, confirming the results. To discuss the usefulness of the selection of an appropriate PLS calibration set, the PTG content was determined by calculating a PLS model based on the previously selected classes. In comparison to a PLS model calculated using a pooled calibration set containing samples from all classes, the root mean square error of prediction could be improved significantly using PLS models based on the selected calibration sets using PLS-DA, ranging between 1.06 and 2.91% (w/w).

  6. In situ study of the photodegradation of carbofuran deposited on TiO2 film under UV light, using ATR-FTIR coupled to HS-MCR-ALS.

    Science.gov (United States)

    Atifi, Abderrahman; Czarnecki, Kazimierz; Mountacer, Hafida; Ryan, Michael D

    2013-08-01

    The in situ study of the photodegradation of carbofuran deposited on a TiO2 catalyst film under UV light was carried out using the ATR-FTIR technique. The data were analyzed using a Hard-Soft Multivariate Curve Resolution-Alternating Least Squares (HS-MCR-ALS) methodology. Using S-MCR-ALS, four factors were deduced from the evolving factor analysis of the data, and their concentrations and spectra were determined. These results were used to draw qualitative and quantitative analyses of the major products of carbofuran photodegradation. The results of this analysis were in good agreement with GC-MS results and with reported mechanisms. Hard-MCR-ALS was then used to refine the spectra and concentrations, using a multistep kinetic model. The rate constant for the first step in the photodegradation of carbofuran was found to be 2.9 × 10(-3) min(-1). The higher magnitude of the correlation (96.87%), the explained variance (99.87%) and LOF (3.01), are good indicators of the reliability of the outcome of this approach. This method has been shown to be an efficient approach to study in situ photodegradation of pesticides on a solid surface.

  7. Attenuated Total Reflection-Fourier Transform Infrared Spectroscopic Study of Dried Shark Fin Products%鱼翅干制品品质的ATR-FTIR鉴别研究

    Institute of Scientific and Technical Information of China (English)

    韩婉清; 罗海英; 冼燕萍; 罗东辉; 穆同娜; 郭新东

    2015-01-01

    Sixty-four pieces of shark fin dried products (including real,fake and artificial shark fin products)and real products coated with gelatin were rapidly and nondestructively analyzed by attenuated total reflection-Fourier transform infrared spectros-copy (ATR-FTIR).The characteristic of IR spectrograms among the above four kinds of samples were systematically studied and comparied,the results showed that the spectrograms of the same kind of samples were repeatable,and different kinds of shark fin products presented significant differences in the spectrograms,which mainly manifested as the specific absorption peaks of amido bonds in protein (1 650,1 544 cm-1 )and skeletal vibration in polysaccharide (1 050 cm-1 ).The spectrograms of real shark fins were characterized by the strong absorption peaks of protein characteristic amide Ⅰ and Ⅱ absorbent (1 650,1 544 cm-1 )and relatively weak C—O—C vibration absorbent (1 050 cm-1 )owing to the high content of protein and relatively low level of polysaccharide.For fake shark fin products that were molded form by mixing together with the offcut of shark,collagen and other substances,the introduction of non-protein materials leaded to the weaker amido bonds absorbent than real products along with a 30 cm-1 blue shift of amide Ⅰabsorbent.Opposite to the real sample,the relatively strong absorption peak of poly-saccharide (~1 047 cm-1 )and barely existed amide absorbent were the key features of the spectrogram of artificial samples, which was synthersized by polysaccharide like sodium alginate.Real samples coated with gelatin,the peak strength of protein and polysaccharide were decreased simultaneously when the data collection was taken at the surface of sample,while the spectro-gram presented no significant difference to real samples when the data was collected in the section.The results above indicated that by analyzing the characteristic of IR spectrograms and the value range of Apro/Apol collected by ATR

  8. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) to study the molecular conformation of parchment artifacts in different macroscopic states.

    Science.gov (United States)

    Gonzalez, Lee; Wade, Matthew; Bell, Nancy; Thomas, Kate; Wess, Tim

    2013-02-01

    Maintaining appropriate temperatures and relative humidity is considered essential to extending the useful life of parchment artifacts. Although the relationship between environmental factors and changes to the physical state of artifacts is reasonably understood, an improved understanding of the relationship between the molecular conformation and changes to the macroscopic condition of parchment is needed to optimize environmental conditions. Using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR FT-IR) analysis, the conformation of the molecular structure in selected parchment samples with specific macroscopic conditions, typically discoloration and planar deformations (e.g., cockling and tearing), have been made. The results of this investigation showed that the Fourier transform infrared signal differs for parchment samples exhibiting different macroscopic conditions. In areas exhibiting planar deformation, a change in the Fourier Transform Infrared signal was observed that indicates unfolding of the molecular conformation. In comparison, the discolored samples showed a change in molecular conformation that indicates a chemical change within the collagen molecular structure. This paper discusses the possible causal associations and implications of these findings for the conservation and preservation of parchment artifacts.

  9. Mangrove dolabrane-type of diterpenes tagalsins suppresses tumor growth via ROS-mediated apoptosis and ATM/ATR-Chk1/Chk2-regulated cell cycle arrest.

    Science.gov (United States)

    Neumann, Jennifer; Yang, Yi; Köhler, Rebecca; Giaisi, Marco; Witzens-Harig, Mathias; Liu, Dong; Krammer, Peter H; Lin, Wenhan; Li-Weber, Min

    2015-12-01

    Natural compounds are an important source for drug development. With an increasing cancer rate worldwide there is an urgent quest for new anti-cancer drugs. In this study, we show that a group of dolabrane-type of diterpenes, collectively named tagalsins, isolated from the Chinese mangrove genus Ceriops has potent cytotoxicity on a panel of hematologic cancer cells. Investigation of the molecular mechanisms by which tagalsins kill malignant cells revealed that it induces a ROS-mediated damage of DNA. This event leads to apoptosis induction and blockage of cell cycle progression at S-G2 phase via activation of the ATM/ATR-Chk1/Chk2 check point pathway. We further show that tagalsins suppress growth of human T-cell leukemia xenografts in vivo. Tagalsins show only minor toxicity on healthy cells and are well tolerated by mice. Our study shows a therapeutic potential of tagalsins for the treatment of hematologic malignancies and a new source of anticancer drugs. PMID:26061604

  10. Kinetic Parameters during Bis-GMA and TEGDMA Monomer Polymerization by ATR-FTIR: The Influence of Photoinitiator and Light Curing Source

    Directory of Open Access Journals (Sweden)

    Aline B. Denis

    2016-01-01

    Full Text Available This study aimed to analyze the kinetic parameters of two monomers using attenuated total reflectance Fourier transform infrared (ATR-FTIR: 2,2-bis-[4-(2-hydroxy-3-methacryloxypropyl-1-oxy-phenyl] propane (Bis-GMA and triethylene glycol dimethacrylate (TEGDMA. The following were calculated to evaluate the kinetic parameters: maximum conversion rate (Rpmax, time at the maximum polymerization rate (tmax, conversion at Rpmax, and total conversion recorded at the maximum conversion point after 300 s. Camphorquinone (CQ and phenyl propanedione (PPD were used in this study as photoinitiators, whereas N,N-dimethyl-p-toluidine (DMPT amine was used as a coinitiator. LED apparatus and halogen lamp were used in turn to evaluate the effect that light source had on the monomer kinetics. The mass concentration ratio for the three resin preparations was 0.7 : 0.3 for Bis-GMA and TEGDMA: R1 (CQ + DMPT, R2 (PPD + DMPT, and R3 (PPD + CQ + DMPT. The PPD association with the CQ photoinitiator altered the polymerization kinetics compared to a resin containing only the CQ photoinitiator. The light sources exhibited no significant differences for tmax of R1 and R3. Resins containing only the PPD initiator exhibited a higher tmax than those containing only CQ. However, the Rpmax decreased for resins containing the PPD photoinitiator.

  11. Glyphosate complexation to aluminium(III). An equilibrium and structural study in solution using potentiometry, multinuclear NMR, ATR-FTIR, ESI-MS and DFT calculations.

    Science.gov (United States)

    Purgel, Mihály; Takács, Zoltán; Jonsson, Caroline M; Nagy, Lajos; Andersson, Ingegärd; Bányai, István; Pápai, Imre; Persson, Per; Sjöberg, Staffan; Tóth, Imre

    2009-11-01

    The stoichiometries and stability constants of a series of Al(3+)-N-phosponomethyl glycine (PMG/H(3)L) complexes have been determined in acidic aqueous solution using a combination of precise potentiometric titration data, quantitative (27)Al and (31)P NMR spectra, ATR-FTIR spectrum and ESI-MS measurements (0.6M NaCl, 25 degrees C). Besides the mononuclear AlH(2)L(2+), Al(H(2)L)(HL), Al(HL)(2)(-) and Al(HL)L(2-), dimeric Al(2)(HL)L(+) and trinuclear Al(3)H(5)L(4)(2+) complexes have been postulated. (1)H and (31)P NMR data show that different isomers co-exist in solution and the isomerization reactions are slow on the (31)P NMR time scale. The geometries of monomeric and dimeric complexes likely double hydroxo bridged and double phosphonate bridged isomers have been optimized using DFT ab initio calculations starting from rational structural proposals. Energy calculations using the PCM solvation method also support the co-existence of isomers in solutions.

  12. A novel mutation in the putative DNA helicase XH2 is responsible for male-to-female sex reversal associated with an atypical form of the ATR-X syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Ion, A.; Telvi, L.; Galacteros, F.; McElreavey, K. [Institut Pasteur, Paris (France)] [and others

    1996-06-01

    We describe a pedigree presenting X-linked severe mental retardation associated with multiple congenital abnormalities and 46,XY gonadal dysgenesis, leading in one family member to female gender assignment. Female carriers are unaffected. The dysmorphic features are similar to those described in the {alpha}-thalassemia and mental retardation (ATR-X) syndrome, although there is no clinical evidence of {alpha}-thalassemia in this family. In addition, the family had other clinical features not previously observed in the ATR-X syndrome, including partial optic-nerve atrophy and partial ocular albinism. Mutations in a putative DNA helicase, termed XH2, have been reported to give rise to the ATR-X syndrome. We screened the YCH2 gene for mutations in affected members of the family and identified a 4-bp deletion at an intron/exon boundary that removes an invariant 3{prime} splice-acceptor site. The mutation cosegregates with the syndrome. The genomic deletion causes missplicing of the pre-mRNA, which results in the loss of 8 bp of coding sequence, thereby generating a frameshift and a downstream premature stop codon. Our finding increases the range of clinical features associated with mutations in the XH2 gene. 17 refs., 4 figs., 2 tabs.

  13. Fuentes de variabilidad en el diagnóstico de gastritis atrófica multifocal asociada con la infección por Helicobacter pylori1

    Science.gov (United States)

    Bravo, Luis Eduardo; Bravo, Juan Carlos; Realpe, José Luis; Zarama, Guillermo; Piazuelo, MarÍa Blanca; Correa, Pelayo

    2014-01-01

    RESUMEN Introducción El mapeo de las diferentes regiones del estómago y el número de fragmentos de mucosa gástrica disponibles para evaluación histopatológica son fuentes importantes de variación en el momento de clasificar y hacer la gradación de la gastritis crónica. Objetivos Estimar la sensibilidad del número de fragmentos de mucosa gástrica necesarios para establecer los diagnósticos de gastritis atrófica con metaplasia intestinal (MI), displasia y estado de infección por Helicobacter pylori. Además evaluar la variabilidad intra-observador en la clasificación de estas lesiones precursoras del cáncer gástrico. Materiales y métodos En una cohorte de 6 años de seguimiento se evaluaron 1,958 procedimientos de endoscopia realizados por dos gastroenterólogos. En cada procedimiento y de cada participante se obtuvieron 5 biopsias de mucosa gástrica que representaban antro, incisura angularis y cuerpo. Un único patólogo hizo la interpretación histológica de las 5 biopsias y proporcionó un diagnóstico definitivo global que se utilizó como patrón de referencia. Cada fragmento de mucosa gástrica examinado condujo a un diagnóstico individual para cada biopsia que se comparó con el patrón de referencia. La variabilidad intra-observador se evaluó en 127 personas que corresponden a una muestra aleatoria de 20% del total de endoscopias hechas a los 72 meses de seguimiento. Resultados La sensibilidad del diagnóstico de MI y displasia gástrica aumentó de manera significativa con el número de fragmentos de mucosa gástrica evaluados El sitio anatómico de mayor sensibilidad para el diagnóstico de MI y displasia fue la incisura angularis. Para descubrir H. pylori se logró alta sensibilidad con el estudio de un solo fragmento de mucosa gástrica (95.9%) y fue independiente del sitio de obtención de la biopsia. El acuerdo intra-observador para el diagnóstico de gastritis crónica fue 86.1% con valor kappa de 0.79 IC 95% (0.76-0.85). Las

  14. Fuentes de variabilidad en el diagnóstico de gastritis atrófica multifocal asociada con la infección por Helicobacter pylori

    Directory of Open Access Journals (Sweden)

    Luis Eduardo Bravo

    2008-03-01

    Full Text Available Introducción: El mapeo de las diferentes regiones del estómago y el número de fragmentos de mucosa gástrica disponibles para evaluación histopatológica son fuentes importantes de variación en el momento de clasificar y hacer la gradación de la gastritis crónica. Objetivos: Estimar la sensibilidad del número de fragmentos de mucosa gástrica necesarios para establecer los diagnósticos de gastritis atrófica con metaplasia intestinal (MI, displasia y estado de infección por Helicobacter pylori. Además evaluar la variabilidad intra-observador en la clasificación de estas lesiones precursoras del cáncer gástrico. Materiales y métodos: En una cohorte de 6 años de seguimiento se evaluaron 1,958 procedimientos de endoscopia realizados por dos gastroenterólogos. En cada procedimiento y de cada participante se obtuvieron 5 biopsias de mucosa gástrica que representaban antro, incisura angularis y cuerpo. Un único patólogo hizo la interpretación histológica de las 5 biopsias y proporcionó un diagnóstico definitivo global que se utilizó como patrón de referencia. Cada fragmento de mucosa gástrica examinado condujo a un diagnóstico individual para cada biopsia que se comparó con el patrón de referencia. La variabilidad intra-observador se evaluó en 127 personas que corresponden a una muestra aleatoria de 20% del total de endoscopias hechas a los 72 meses de seguimiento.Resultados: La sensibilidad del diagnóstico de MI y displasia gástrica aumentó de manera significativa con el número de fragmentos de mucosa gástrica evaluados El sitio anatómico de mayor sensibilidad para el diagnóstico de MI y displasia fue la incisura angularis. Para descubrir H. pylori se logró alta sensibilidad con el estudio de un solo fragmento de mucosa gástrica (95.9% y fue independiente del sitio de obtención de la biopsia. El acuerdo intra-observador para el diagnóstico de gastritis crónica fue 86.1% con valor kappa de 0.79 IC 95% (0

  15. Artesunate induces oxidative DNA damage, sustained DNA double-strand breaks, and the ATM/ATR damage response in cancer cells.

    Science.gov (United States)

    Berdelle, Nicole; Nikolova, Teodora; Quiros, Steve; Efferth, Thomas; Kaina, Bernd

    2011-12-01

    Artesunate, the active agent from Artemisia annua L. used in the traditional Chinese medicine, is being applied as a first-line drug for malaria treatment, and trials are ongoing that include this drug in cancer therapy. Despite increasing interest in its therapeutic application, the mode of cell killing provoked by artesunate in human cells is unknown. Here, we show that artesunate is a powerful inducer of oxidative DNA damage, giving rise to formamidopyrimidine DNA glycosylase-sensitive sites and the formation of 8-oxoguanine and 1,N6-ethenoadenine. Oxidative DNA damage was induced in LN-229 human glioblastoma cells dose dependently and was paralleled by cell death executed by apoptosis and necrosis, which could be attenuated by radical scavengers such as N-acetyl cysteine. Oxidative DNA damage resulted in DNA double-strand breaks (DSB) as determined by γH2AX foci that colocalized with 53BP1. Upon chronic treatment with artesunate, the level of DSB continuously increased over the treatment period up to a steady-state level, which is in contrast to ionizing radiation that induced a burst of DSB followed by a decline due to their repair. Knockdown of Rad51 by short interfering RNA and inactivation of DNA-PK strongly sensitized glioma cells to artesunate. These data indicate that both homologous recombination and nonhomologous end joining are involved in the repair of artesunate-induced DSB. Artesunate provoked a DNA damage response (DDR) with phosphorylation of ATM, ATR, Chk1, and Chk2. Overall, these data revealed that artesunate induces oxidative DNA lesions and DSB that continuously increase during the treatment period and accumulate until they trigger DDR and finally tumor cell death. PMID:21998290

  16. Optimized data analysis algorithm for on-site chemical identification using a hand-held attenuated total reflection Fourier transform infrared (ATR FT-IR) spectrometer.

    Science.gov (United States)

    Ron, Izhar; Zaltsman, Amalia; Kendler, Shai

    2013-12-01

    On-site identification of organic compounds in the presence of interfering materials using a field-portable attenuated total reflection Fourier transform infrared (ATR FT-IR) spectrometer is presented. Identification is based on an algorithm that compares the analyte's infrared absorption spectrum with the reference spectra. The comparison is performed at several predetermined frequencies, and a similarity value (distance) between the measured and the reference spectra is calculated either at each frequency individually, or, alternatively, the average distance for all frequencies is calculated. The examined frequencies are selected to give the best contrast between the target materials of interest. In this study, the algorithm was optimized to identify three common chemical warfare agents (CWAs): O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothioic acid (VX), sarin (GB), and sulfur mustard (bis(2-chloroethyl) sulfide) (HD), in the presence of field-related interfering materials (fuels, water, and dust). Receiver operating characteristics analysis was performed in order to determine the probabilities for detection (PD) and for false alerts (PF). Challenging the algorithm with a set of data that contains mixtures of CWAs and interfering materials resulted in PD of 90% and PF of 0%, 0%, and 1% for VX, GB, and HD, respectively, using the average distance approach, which was found to be much more effective than analyzing each frequency individually. This finding was validated for all possible combinations of 2-7 peaks per material. It is suggested that this algorithm provides a reliable mean for the identification of a predetermined set of target analytes and interfering materials.

  17. CUANDO LA MEMORIA POÉTICA DOCUMENTA LA HISTORIA NO TODO LO SÓLIDO SE DESVANECE EN EL AIRE: (En torno a Nada queda atrás de Carlos Trujillo y Milton Rogovin When poetic memory documents history not everything solid disappears in the air: (Regarding Nada Queda Atrás, by Carlos Trujillo and Milton Rogovin

    Directory of Open Access Journals (Sweden)

    Sergio Mansilla Torres

    2009-07-01

    Full Text Available Nada queda atrás es un libro de fotografías documentales y de poesía. Las fotografías, tomadas por Milton Rogovin en 1967, en la localidad de Quemchi, Chiloé, son hoy documentos históricos y de denuncia de las condiciones de pobreza en las que entonces vivía la población campesina y pescadora de Chiloé. Los poemas, escritos por Carlos Trujillo 37 años después en los Estados Unidos, son, por su lado, testimonios poéticos de la memoria, personal y colectiva, que suscitan las fotografías; memoria que, a su turno, se vuelve (reconstrucción imaginaria de la identidad cultural de Chiloé, vivida por el poeta, como experiencia singular de extranjería y de ausencia. Los textos poéticos son, pues, derivaciones del documento fotográfico, pero, al mismo tiempo, son señas para ver/leer las fotografías como metáforas del Chiloé profundo de ayer y de hoy. Visto así, el libro constituye, literalmente, un documento de cultura y de barbarie: cultura en tanto el libro es un reconocimiento ético y estético a “los olvidados” y barbarie en tanto ese reconocimiento delata historias de postergación y de desigualdad.Nada queda atrás is a book of documentary photographs and poetry. The photograghs, shot by Milton Rogovin in 1967, in Quemchi on the island of Chiloé, are today documents which record history and denounce the empoverished conditions in which the fishermen and farmers then lived in Chiloé. The poems, written by Carlos Trujillo, 37 years later in the United Status are, on the one hand, poetic testimonies of the personal and collective memory that the photographs suggest; memories which, in turn, become an imaginary (reconstruction of the cultural identity of Chiloé lived by the poet, as a particular experience of foreignity and absence. The poetic texts are, then, derivations of the photographic document, but at the same time they are symbols with which to read/see the photos as metaphors of a profound Chiloé of olden times

  18. eds1、atr/nrc1、ahl19三个抗病相关的防卫基因的研究进展%Progress on Three Defence Genes eds1, atr/nrc1 and ahl19

    Institute of Scientific and Technical Information of China (English)

    齐希梁; 程红梅

    2013-01-01

    防卫基因在植物抗病分子生物学的的研究中起到重要的作用。本文综述了抗病相关基因eds1、atr/nrc1、ahl19在防卫病原微生物入侵时的机理及在信号通路中的作用。 EDS1是依赖水杨酸的抗性途径TIR-NB-LRR类R蛋白防御中的一个必需的基本元件;AHL19是防卫病原菌入侵时是一个关键的调控元件,防卫病原菌入侵的信号通路还有待探索;NRC1是HR关联的细胞程序性死亡的抗性蛋白Cf-9、Pto和Rx途径中的必需因子。这些基因的研究有助于植物抗病分子育种研究,尤其是针对缺少抗原的重大病害(如棉花黄萎病)提供了获得转基因新品种的可能性。%Defence genes play an important role in plant resistant to disease. In this paper we introduce three defence genes’ (eds1,atr/nrc1 and ahl19) function in defenses to pathogens and the role in their signal pathway. EDS1 encodes an essential component of disease resistant R genes in salicylic acid dependent resistance pathway TIR-NB-LRR class. AHL19 acts as a positive regulator of plant defense. NRC1 is also required for the HR pathway induced by the R proteins Cf-9, Pto and Rx. It provides theoretical basis to explore the mechanism of plant resistance to disease, especially to the plants which lack the antigen to the disease such as cotton Verticillium Wilt. It is possible to breed new varieties with high resistance to Verticillium Wilt of cotton by using those defence genes.

  19. Determinação quantitativa da concentração de silicone em antiespumantes por espectroscopia FT-IR / ATR e calibração multivariada Quantitative determination of silicone in antifoaming products by FT-IR / ATR spectroscopy and multivariate calibration

    Directory of Open Access Journals (Sweden)

    Marcelo H. F. Garcia

    2004-12-01

    Full Text Available Neste trabalho apresentamos uma alternativa para a dosagem do teor de silicone (polidimetilsiloxano em antiespumantes por meio da técnica de espectroscopia no infravermelho com transformada de Fourier (FT-IR, com a utilização do acessório de reflectância total atenuada (ATR. Os espectros foram registrados na faixa espectral de 2500 a 780 cm-1, com resolução de 4 cm-1 e 128 varreduras. A calibração de um modelo linear por meio da utilização do método de mínimos quadrados parciais (PLS aplicado aos espectros foi capaz de determinar satisfatoriamente a concentração de silicone nas amostras. Este método é de extrema importância para indústrias produtoras de antiespumantes siliconados, uma vez que o desempenho de tais produtos geralmente é avaliado em função da viscosidade dos mesmos. Muitas vezes no processo de fabricação de tais produtos ocorre uma homogeneização incompleta do silicone no solvente, o que leva a resultados de viscosidade que não são representativos das amostras analisadas. A determinação da concentração do teor de silicone é uma importante ferramenta para o Controle Estatístico de Processo (CEP, pois evita o desperdício de matérias-primas empregadas na fabricação dos antiespumantes.This work presents an alternative method to determine the concentration of silicone (polydimethylsiloxane in antifoaming products using Fourier Transformed Infrared Spectroscopy (FT-IR with the attenuated total reflectance (ATR accessory. The spectra were recorded in the range from 2500 to 780 cm-1, with a resolution of 4 cm-1 and 128 scans. With calibration of a linear model using PLS regression method applied to spectral data we were able to determine the silicone concentration in the samples. This method may be useful for antifoaming producers since the performance of such products generally is evaluated as a function of their viscosity. Moreover, during manufacturing an incomplete homogenization of silicone in the

  20. Using ATR-FT/IR molecular spectroscopy to detect effects of blend DDGS inclusion level on the molecular structure spectral and metabolic characteristics of the proteins in hulless barley

    Science.gov (United States)

    Zhang, Xuewei; Yu, Peiqiang

    2012-09-01

    The objectives of this study were to investigate the effects of inclusion of a bioethanol co-product of blend DDGS (wheat:corn = 70%:30%) on protein molecular structure spectral and metabolic characteristics in hulless barley-based feed using ATR-FT/IR molecular spectroscopy. Hulless barley grain with the blend DDGS were mixed in the five ratios. The results showed that when blend DDGS was included at an increased ratio, predicted truly absorbed protein supply was highly and linearly increased (P products for dairy and beef cattle.

  1. Impact of cloud processes on aerosol particle properties: results from two ATR-42 flights in an extended stratocumulus cloud layer during the EUCAARI campaign (2008

    Directory of Open Access Journals (Sweden)

    S. Crumeyrolle

    2011-12-01

    Full Text Available Within the European Aerosol Cloud Climate and Air Quality Interactions (EUCAARI project, the Meteo France research aircraft ATR-42 was operated from Rotterdam airport during May 2008, to perform scientific flights dedicated to the investigation of aerosol-cloud interactions. Therein, the objective of this study is to illustrate the impact of cloud processing on the aerosol particles physical and chemical properties. The presented results are retrieved from measurements during a double-flight mission from Rotterdam (Netherlands to Newcastle (UK and back using data measured with compact Time of Flight Aerosol Mass Spectrometer (cToF-AMS and Scanning Mobility Particle Sizer (SMPS. Cloud-related measurements during these flights were performed over the North Sea within as well as in close vicinity of a marine stratocumulus cloud layer. Particle physical and chemical properties observed in the close vicinity (V, below and above the stratocumulus cloud show strong differences. Firstly, measurements at constant altitude above the cloud layer show decreasing mass concentrations with decreasing horizontal distance (210–0 km to the cloud layer by a factor up to 7, whereas below the cloud and by same means of distance, the mass concentrations merely decrease by a factor of 2 on average. Secondly, the averaged aerosol size distributions, observed above and below the cloud layer, are of bimodal character with pronounced minima between Aitken and accumulation mode which is potentially the consequence of cloud processing. Finally, the chemical composition of aerosol particles is strongly dependent on the location relative to the cloud layer (vicinity or below/above cloud. In general, the nitrate and organic fractions decrease with decreasing distance to the cloud, in the transit from cloud–free conditions towards the cloud boundaries. The decrease of nitrate and organic compounds ranges at a factor of three to ten, affecting sulfate and ammonium compounds

  2. Ablation of burned skin with ultra-short pulses laser to promote healing: evaluation by optical coherence tomography, histology, μATR-FTIR and Nonlinear Microscopy

    International Nuclear Information System (INIS)

    Burns cause changes in the anatomical structure of the skin associated with trauma. The severity of the burn injury is divided into first, second and third-degree burns. The third-degree burns have been a major focus of research in search of more conservative treatments and faster results in repair for a functional and cosmetically acceptable. The conventional treatment is the use of topical natural or synthetic skin graft. An alternative therapy is the laser ablation process for burned tissue necrosis removal due to the no mechanical contact, fast application and access to difficult areas. The purpose of this study is to evaluate the feasibility of using high intensity femtosecond lasers as an adjunct treatment of burned patients. For this study, 65 Wistar rats were divided into groups of five animals: healthy skin, burned skin, two types of treatment (surgical debridement or femtosecond laser ablation) and four different times in the healing process monitoring. Three regions of the back of the animals were exposed to steam source causing third-degree burn. On the third day after the burn, one of the regions was ablated with high intensity ultrashort laser pulses (λ = 785 nm, 90 fs, 2 kHz and 10 μJ/ pulse), the other received surgical debridement, and the last was considered the burn control. The regions were analyzed by optical coherence tomography (OCT), histology, attenuated total reflectance infrared spectroscopy using Fourier transform (μ-ATR-FTIR), two-photon excitation fluorescence microscopy (TPEFM) and second harmonic generation technique (SHG) on days 3, 5, 7 and 14 pos-treatments. The results showed that with the laser irradiation conditions used it was possible to remove debris from third degree burn. The techniques used to characterize the tissue allowed to verify that all treatments promoted wound healing. On the fourteenth day, the regeneration curve showed that the attenuation coefficient of laser ablated tissue converges to the values of

  3. AGR-2 Data Qualification Report for ATR Cycles 151B-2, 152A, 152B, 153A, 153B and 154A

    Energy Technology Data Exchange (ETDEWEB)

    Binh T. Pham; Jeffrey J. Einerson

    2013-09-01

    This report documents the data qualification status of AGR-2 fuel irradiation experimental data from Advanced Test Reactor (ATR) Cycles 152A, 152B, 153A, 153B, and 154A, as recorded in the Nuclear Data Management and Analysis System (NDMAS). The AGR-2 data streams addressed include thermocouple (TC) temperatures, sweep gas data (flow rate, pressure, and moisture content), and fission product monitoring system (FPMS) data for each of the six capsules in the experiment. A total of 13,400,520 every minute instantaneous TC and sweep gas data records were received and processed by NDMAS for this period. Of these data, 8,911,791 records (66.5% of the total) were determined to be Qualified based on NDMAS accuracy testing and data validity assessment. For temperature, there were 4,266,081 records (74% of the total TC data) that were Failed due to TC instrument failures. For sweep gas flows, there were 222,648 gas flow records (2.91% of the flow data) that were Failed. The inlet gas flow failures due to gas flow cross-talk and leakage problems that occurred after Cycle 150A were corrected by using the same gas mixture in all six capsules and the Leadout. For FPMS data, NDMAS received and processed preliminary release rate and release-to-birth rate ratio (R/B) data for three reactor cycles (Cycles 149B, 150B, and 151A) . This data consists of 45,983 release rate records and 45,235 R/B records for the 12 radionuclides reported. The qualification status of these FPMS data has been set to In Process until receipt of Quality Assurance-approved data generator reports. All of the above data have been processed and tested using a SAS®-based enterprise application software system, stored in a secure Structured Query Language database, made available on the NDMAS Web portal (http://ndmas.inl.gov), and approved by the INL STIM for release to both internal and appropriate external Very High Temperature Reactor Program participants.

  4. Automatic Traffic Recorder (ATR) Stations

    Data.gov (United States)

    Department of Homeland Security — The data included in the GIS Traffic Stations Version database have been assimilated from station description files provided by FHWA for Weigh-in-Motion (WIM), and...

  5. ATR alterations in Hodgkin's lymphoma

    NARCIS (Netherlands)

    Liu, Angen; Takakuwa, Tetsuya; Fujita, Shigeki; Luo, Wen-Juan; Tresnasari, Kristianti; Van den Berg, Anke; Poppema, Sibrand; Aozasa, Katsuyuki

    2008-01-01

    Hodgkin's lymphoma (HL) is characterized by the presence of neoplastic Hodgkin and Reed-Sternberg cells (HRSC) in a background of inflammatory cells. Free radicals and oxidative stress generated in the inflammatory lesions could cause DNA damage, thus providing a basis for lymphomagenesis. Ataxia-te

  6. Effect of pH on the Nitrite Hydrogenation Mechanism over Pd/Al2O3 and Pt/Al2O3: Details Obtained with ATR-IR Spectroscopy

    DEFF Research Database (Denmark)

    Ebbesen, Sune Dalgaard; Mojet, Barbara L.; Lefferts, Leon

    2011-01-01

    It is well-known that activity and selectivity to N2 during nitrite hydrogenation over noble metal catalysts in water depend on the pH of the solution, but mechanistic understanding is lacking. Attenuated total reflection infrared (ATR-IR) spectroscopy is an ideal tool to perform detailed studies...... on catalytic surfaces in water. In this paper, the influence of pH was studied on adsorption and subsequent hydrogenation of nitrite in water between pH 5 and 9 over Pd/Al2O3 and Pt/Al2O3, using ATR-IR spectroscopy. On both catalysts, pH clearly influenced the surface coverage and reaction rates...... of intermediates. For Pt/Al2O3, lowering the pH induced the increasing surface coverage of key reaction intermediates like NOsteps1620cm−1 and “HNO”(ads)1540cm−1, as well as increased hydrogenation rates, explaining the higher TOF at lower pH as reported in the literature. For Pd/Al2O3, the effect of pH...

  7. 冬凌草甲素通过激活ATR通路诱导HEPG2细胞凋亡实验研究%Oridonin Induced the HEPG2 Cells Apoptosis by the Activation of the ATR Pathway

    Institute of Scientific and Technical Information of China (English)

    周泽雄; 曲珍仪; 刘颖

    2016-01-01

    Objective:To research oridonin induce the HEPG2 cells apoptosis and its the related mechanism.Methods:Using MTT and crystal violet staining to study oridonin inhibited HEPG2 cells proliferative,Western Blotting to detect the change of ATR,H2AX,γ-H2AX,P53 and so on protein after oridonin treated HEPG2 cells.Results:MTT method and crystal violet staining show that oridonin can significantly inhibited HEPG2 cells,and the inhibition effect in certain concentrations with a dosedependent manner.Western blotting showed that the ATR,P-P53,γ-H2AX protein activity and express has significant enhanced during oridonin induced the HEPG2 cells apoptosis.Conclusion:Oridonin induced the HEPG2 cells apoptosis may be cause by the phosphorylation of H2AX protein further activate the ATR signaling pathways lead to cell death.%目的:探讨冬凌草甲素抑制HEGP2肝癌细胞增殖及其机制研究方法:MTT法检测抑制率、结晶紫检测冬凌草甲素诱导HEPG2肝癌细胞抑制增殖作用、Western blotting检测不同浓度冬凌草甲素作用HEPG2肝癌细胞后ATR、H2AX、γ-H2AX、P53等蛋白的变化.结果:MTT法、结晶紫法显示冬凌草甲素对HEPG2肝癌细胞能够明显抑制,且抑制作用在一定浓度范围内呈计量依赖性;Western blotting结果显示冬凌草甲素在诱导细胞凋亡过程中ATR、P-P53、γ-H2AX表达水平及活性显著增强.结论:冬凌草甲素诱导HEPG2肝癌细胞发生凋亡可能是通过引起H2AX蛋白磷酸化进一步激活ATR信号通路引起细胞凋亡.

  8. Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR FT-IR) Mapping Coupled with Multivariate Curve Resolution (MCR) for Studying the Miscibility of Chlorobutyl Rubber/Polyamide-12 Blends.

    Science.gov (United States)

    Tang, Yongjiao; Jing, Nan; Zhang, Pudun

    2015-11-01

    A series of chlorobutyl rubber/polyamide-12 (CIIR/PA-12) blends compatibilized by different amounts of maleic anhydride (MAH) grafted polypropylene (PP-g-MAH) were investigated by attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) mapping. Multivariate curve resolution (MCR) was used to process the FT-IR images. Both the spectra of pure components in the blends and their concentration distributions in a micro-region were acquired. Our results demonstrated that the blend with 15 parts per hundred rubber PP-g-MAH showed the best miscibility. An amide interphase and an imide interphase were inferred by analyzing the spectra of MCR component 3 of the blends with and without PP-g-MAH, respectively. Correspondingly, two different compatibilizing mechanisms were proposed for these blends. PMID:26647055

  9. Estimativa dos índices de pneumonia, pela tosse, e de rinite atrófica, por espirros, em suínos Estimating of pneumonia by coughing and atrophic rhinitis by sneezing indices in swine

    OpenAIRE

    N. Morés; W. Barioni Junior; J. Sobestansky; O.A. Dalla Costa; I.A. Piffer; D.P. Paiva; R. Guzzo; J.B.S. Coimbra

    2001-01-01

    Realizou-se um estudo em 64 granjas de suínos na região sul do Brasil entre julho de 1995 e março de 1997 com o objetivo de estimar o índice de pneumonia (IP) e o índice de rinite atrófica (IRA), por meio da contagem dos sinais clínicos de tosse e espirro, respectivamente, em suínos em crescimento-terminação. Em cada granja, um lote com cerca de 60 suínos foi acompanhado desde o alojamento na fase de crescimento até o abate. Tosse e espirro foram contados em quatro oportunidades (30, 50 e 80 ...

  10. Ablation of burned skin with ultra-short pulses laser to promote healing: evaluation by optical coherence tomography, histology, {mu}ATR-FTIR and Nonlinear Microscopy; Ablacao de pele queimada com laser de pulsos ultra-curtos para promocao da cicatrizacao: avaliacao por tomografia por coerencia optica, histologia, {mu}ATR-FTIR e microscopia nao-linear

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Moises Oliveira dos

    2012-07-01

    Burns cause changes in the anatomical structure of the skin associated with trauma. The severity of the burn injury is divided into first, second and third-degree burns. The third-degree burns have been a major focus of research in search of more conservative treatments and faster results in repair for a functional and cosmetically acceptable. The conventional treatment is the use of topical natural or synthetic skin graft. An alternative therapy is the laser ablation process for burned tissue necrosis removal due to the no mechanical contact, fast application and access to difficult areas. The purpose of this study is to evaluate the feasibility of using high intensity femtosecond lasers as an adjunct treatment of burned patients. For this study, 65 Wistar rats were divided into groups of five animals: healthy skin, burned skin, two types of treatment (surgical debridement or femtosecond laser ablation) and four different times in the healing process monitoring. Three regions of the back of the animals were exposed to steam source causing third-degree burn. On the third day after the burn, one of the regions was ablated with high intensity ultrashort laser pulses ({lambda} = 785 nm, 90 fs, 2 kHz and 10 {mu}J/ pulse), the other received surgical debridement, and the last was considered the burn control. The regions were analyzed by optical coherence tomography (OCT), histology, attenuated total reflectance infrared spectroscopy using Fourier transform ({mu}-ATR-FTIR), two-photon excitation fluorescence microscopy (TPEFM) and second harmonic generation technique (SHG) on days 3, 5, 7 and 14 pos-treatments. The results showed that with the laser irradiation conditions used it was possible to remove debris from third degree burn. The techniques used to characterize the tissue allowed to verify that all treatments promoted wound healing. On the fourteenth day, the regeneration curve showed that the attenuation coefficient of laser ablated tissue converges to the values

  11. The Introduction of ATR Instrumentation Design in the Methanol Plant Based on Coke-oven Gas%焦炉气制甲醇装置中ATR仪表设计浅谈

    Institute of Scientific and Technical Information of China (English)

    余博

    2011-01-01

    焦炉气是炼焦过程的副产品,除部分返回炼焦炉用作加热燃料外,剩余的气体主要用作城市居民的燃料气,也有相当数量通过火炬燃烧放空.随着国家节能减排的深入,利用焦炉气制甲醇一方面减少对大气CO2的排放,另一方面还可以产生经济效益.开发了纯氧部分氧化制合成气的技术,在焦炉气制甲醇装置中采取ATR技术,其主要包括仪表选型、复杂控制及联锁逻辑三方面的内容.合理的设计实现对装置的工艺过程实时监控、设备的安全运行,提高工艺气转化率.%The coke oven gas is the by-product in coking process and is flammable.Partial coke oven gas returns to coke burner as heating fuel, the surplus gas is largely applied as urban fuel gas, and quite a lot is vented to flare stack.With the support from the state for energy-saving and pollution-reducing project, the methanol project based on coke oven gas is a favorble option: it can reduce CO2 emmission and also yield economic benefit.After years' exploration and research, a synthetic gas production technology by the partial oxidation of pure oxygen has been developed, therefore ATR technology will be widely applied in this process.Instrumentation design plays an important role in application of ATR technology.

  12. Simulated Microgravity Promotes Cell Apoptosis Through Suppressing Uev1A/TICAM/TRAF/NF-κB-Regulated Anti-Apoptosis and p53/PCNA- and ATM/ATR-Chk1/2-Controlled DNA-Damage Response Pathways.

    Science.gov (United States)

    Zhao, Tuo; Tang, Xin; Umeshappa, Channakeshava Sokke; Ma, Hong; Gao, Haijun; Deng, Yulin; Freywald, Andrew; Xiang, Jim

    2016-09-01

    Microgravity has been known to induce cell death. However, its underlying mechanism is less studied. In this study, BL6-10 melanoma cells were cultured in flasks under simulated microgravity (SMG). We examined cell apoptosis, and assessed expression of genes associated with apoptosis and genes regulating apoptosis in cells under SMG. We demonstrate that SMG induces cell morphological changes and microtubule alterations by confocal microscopy, and enhances apoptosis by flow cytometry, which was associated with up- and down-regulation of pro-apoptotic and anti-apoptotic genes, respectively. Moreover, up- and down-regulation of pro-apoptotic (Caspases 3, 7, 8) and anti-apoptotic (Bcl2 and Bnip3) molecules was confirmed by Western blotting analysis. Western blot analysis also indicates that SMG causes inhibition of an apoptosis suppressor, pNF-κB-p65, which is complemented by the predominant localization of NF-κB-p65 in the cytoplasm. SMG also reduces expression of molecules regulating the NF-κB pathway including Uev1A, TICAM, TRAF2, and TRAF6. Interestingly, 10 DNA repair genes are down-regulated in cells exposed to SMG, among which down-regulation of Parp, Ercc8, Rad23, Rad51, and Ku70 was confirmed by Western blotting analysis. In addition, we demonstrate a significant inhibition of molecules involved in the DNA-damage response, such as p53, PCNA, ATM/ATR, and Chk1/2. Taken together, our work reveals that SMG promotes the apoptotic response through a combined modulation of the Uev1A/TICAM/TRAF/NF-κB-regulated apoptosis and the p53/PCNA- and ATM/ATR-Chk1/2-controlled DNA-damage response pathways. Thus, our investigation provides novel information, which may help us to determine the cause of negative alterations in human physiology occurring at spaceflight environment. J. Cell. Biochem. 117: 2138-2148, 2016. © 2016 Wiley Periodicals, Inc. PMID:26887372

  13. Detect changes in lipid-related structure of brown- and yellow-seeded Brassica Carinata seed during rumen fermentation in relation to basic chemical profile using ATR-FT/IR molecular spectroscopy with chemometrics

    Science.gov (United States)

    Xin, Hangshu; Yu, Peiqiang

    2014-12-01

    In this experiment, brown- and yellow-seeded Brassica carinata were selected to use as a model to investigate whether there were any changes in lipid-related structure make-up (including CH3 and CH2 asymmetric and symmetric stretching bands ca. 3010-2765 cm-1, unsaturated lipid band ca. 3043-2987 cm-1 and carbonyl Cdbnd O ester band ca. 1789-1701 cm-1) of oilseed tissue during rumen in situ incubation using attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FT/IR). Correlations of lipid spectral characteristics with basic chemical profile and multivariate analyses for clarifying structural differences within lipid regions between two carinata seeds were also measured. The results showed that most spectral parameters in both carinata seeds were reduced as incubation time increased. However, the extent of changes in peak intensity of carbonyl Cdbnd O ester group of brown-seeded carinata was not in fully accordance with that of yellow-seeded carinata. Additionally, these lipid structure features were highly correlated with the concentrations of OM (positively), CP (positively), NDF (negatively) and EE (positively) in carinata seeds after 0, 12, 24 and 48 h of incubation. Based on the results from multivariate analyses, neither AHCA nor PCA could produce any distinctions in rumen residues between brown- and yellow-seeded carinata in spectra at lipid regions. It was concluded that besides for original feed samples, spectroscopic technique of ATR-FT/IR could also be used for rumen degradation residues in detecting changes in lipid-related molecular structure make-up. Further studies are needed to explore more details in lipid metabolism during ruminal fermentation with the combined consideration on both metabolic basis and molecular structural basis.

  14. Characterization of silver halide fiber optics and hollow silica waveguides for use in the construction of a mid-infrared attenuated total reflection fourier transform infrared (ATR FT-IR) spectroscopy probe.

    Science.gov (United States)

    Damin, Craig A; Sommer, André J

    2013-11-01

    Advances in fiber optic materials have allowed for the construction of fibers and waveguides capable of transmitting infrared radiation. An investigation of the transmission characteristics associated with two commonly used types of infrared-transmitting fibers/waveguides for prospective use in a fiber/waveguide-coupled attenuated total internal reflection (ATR) probe was performed. Characterization of silver halide polycrystalline fiber optics and hollow silica waveguides was done on the basis of the transmission of infrared light using a conventional fiber optic coupling accessory and an infrared microscope. Using the fiber optic coupling accessory, the average percent transmission for three silver halide fibers was 18.1 ± 6.1% relative to a benchtop reflection accessory. The average transmission for two hollow waveguides (HWGs) using the coupling accessory was 8.0 ± 0.3%. (Uncertainties in the relative percent transmission represent the standard deviations.) Reduced transmission observed for the HWGs was attributed to the high numerical aperture of the coupling accessory. Characterization of the fibers/waveguides using a zinc selenide lens objective on an infrared microscope indicated 24.1 ± 7.2% of the initial light input into the silver halide fibers was transmitted. Percent transmission obtained for the HWGs was 98.7 ± 0.1%. Increased transmission using the HWGs resulted from the absence or minimization of insertion and scattering losses due to the hollow air core and a better-matched numerical aperture. The effect of bending on the transmission characteristics of the fibers/waveguides was also investigated. Significant deviations in the transmission of infrared light by the solid-core silver halide fibers were observed for various bending angles. Percent transmission greater than 98% was consistently observed for the HWGs at the bending angles. The combined benefits of high percent transmission, reproducible instrument responses, and increased bending

  15. Conservation of Moroccan manuscript papers aged 150, 200 and 800 years. Analysis by infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), and scanning electron microscopy energy dispersive spectrometry (SEM-EDS).

    Science.gov (United States)

    Hajji, Latifa; Boukir, Abdellatif; Assouik, Jamal; Lakhiari, Hamid; Kerbal, Abdelali; Doumenq, Pierre; Mille, Gilbert; De Carvalho, Maria Luisa

    2015-02-01

    The preservation of manuscripts and archive materials is a serious problem for librarians and restorers. Paper manuscript is subjected to numerous degradation factors affecting their conservation state. This research represents an attempt to evaluate the conservation restoration process applied in Moroccan libraries, especially the alkaline treatment for strengthening weakened paper. In this study, we focused on six samples of degraded and restored paper taken from three different Moroccan manuscripts aged 150, 200 and 800 years. In addition, the Japanese paper used in restoration has been characterized. A modern paper was also analyzed as reference. A three-step analytical methodology based on infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD) and scanning electron microscopy coupled to energy dispersive spectrometry (SEM-EDS) analysis was developed before and after restoration in order to determine the effect of the consolidation treatment on the paper structure. The results obtained by XRD and ATR-FTIR disclosed the presence of barium sulfate (BaSO4) in all restored paper manuscripts. The presence of calcium carbonate (CaCO3) in all considered samples was confirmed by FTIR spectroscopy. The application of de-acidification treatment causes significant changes connected with the increase of intensity mostly in the region 1426 cm(-1), assigned to the asymmetric and symmetric CO stretching mode of calcite, indicating the effectiveness of de-acidification procedure proved by the rise of the alkaline reserve content allowing the long term preservation of paper. Observations performed by SEM magnify the typical paper morphology and the structure of fibbers, highlighting the effect of the restoration process, manifested by the reduction of impurities.

  16. Khazana Warna Berdasarkan Hasana Alam dan Budaya Nusantara (1

    Directory of Open Access Journals (Sweden)

    Mita Purbasari Wahidiyat

    2011-03-01

    on character, nature, and imagery it evokes. This research will use quantitative and qualitative methods to describe the color names and color names of the new proposed based on the audience choice. Data collected will be used as a proposal of naming colors in Indonesian language that uses the unique natural and cultural riches of Indonesia itself.

  17. Perbedaan Post Purchase Regret Berdasarkan Perilaku Pada Konsumen Wanita

    OpenAIRE

    Fardhani, Diah

    2014-01-01

    Post purchase regret is a negative emotion of consumer after evaluating the purchase decision has been made, the evaluation is conducted based on the outcome and process of consumer decision making. This study was a comparative quantitative study aimed to determine differences on post purchase regret based on unplanned and substitute purchasing behavior. The research hypothesis was the differences in post purchase regret based on unplanned behavior and substitute purchasing. The study involve...

  18. PEMETAAN PREFERENSI KONSUMEN SUPERMARKET DENGAN METODE KANO BERDASARKAN DIMENSI SERVQUAL

    Directory of Open Access Journals (Sweden)

    Kriswanto Widiawan

    2004-01-01

    Full Text Available The management of supermarket usually has some assumptions about the customers' expectation. Sometimes these assumtions do not reflect the nature of customers' expectations. Because of this miss perception, the supermarket looses its profit and becomes inefficient. This research was carried out in order to find out the customers' expectation on facilities and services of the supermarkets according to the servqual dimensions. The outcomes will be mapped into Kano categorizations. The researcher asked the customer dan manager of four supermarkets to fill the prepared questionnaires. Based on the result of customers' questionnaires, tangible, reliability and assurance dimensions were categorized as one dimensional. While responsiveness and emphaty dimensions were categorized as indifferent. Based on the result of managers' questionnaires, all servqual dimensions were categorized as one dimensional. After comparing the results of customers' and managers' questionnaires, there are three of four supermarkets which are match each other. Abstract in Bahasa Indonesia : Manajemen supermarket pada umumnya memiliki asumsi-asumsi mengenai harapan konsumen yang belum tentu sama dengan harapan konsumen sesungguhnya. Akibat ketidaktahuan informasi penting tersebut, tidak sedikit supermarket yang mengalami kerugian atau kinerjanya tidak efisien. Tujuan penelitian ini adalah mengetahui harapan konsumen tentang fasilitas dan layanan supermarket menurut dimensi servqual yang dipetakan ke dalam kategori Kano. Penelitian dilakukan terhadap konsumen dan manajer dengan cara memberikan kuesioner. Menurut responden konsumen supermarket, dimensi servqual yang tergolong kategori one dimensional adalah tangible, reliability dan assurance. Sedangkan dimensi responsiveness dan emphaty termasuk kategori indifferent. Sementara itu, responden manajer supermarket berpendapat semua dimensi servqual termasuk kategori one dimensional. Dari empat supermarket yang diteliti, diketahui ada tiga supermarket yang cocok persepsinya antara manajer dengan konsumennya. Kata kunci: Dimensi servqual, metode Kano, kepuasan konsumen.

  19. PENGELOMPOKKAN KABUPATEN DI PROVINSI BALI BERDASARKAN PERKEMBANGAN FASILITAS PARIWISATA

    Directory of Open Access Journals (Sweden)

    NOVA SARI BARUS

    2013-11-01

    Full Text Available The development of tourism facilities in each district/city between districts with each district is different. The purpose of this study was to determine the grouping of several districts in the province of Bali is based on the number of tourism facilities as well as what the variable identifier of each group. The analysis used in this study is the analysis of clusters and Biplot. The data used is secondary data obtained from the Bali Tourism Office in 2011. Variables used are classified hotel, non-classified hotels, home stays, restaurants, bars, tourist destination, the area of ??tourism, water tourism and business. The results of this study formed three groups: group 1 Bangli, Buleleng, Jembrana District, Klungkung, Karangasem district and Denpasar City. Group 2 and group 3 Badung district of Gianyar district. Variable identifier is a restaurant for all variables.

  20. Redesain Meja dan Kursi Kelas Berdasarkan Antropometri Siswa SDN 060796

    OpenAIRE

    Silvia

    2015-01-01

    Perabot kelas, yang terdiri dari meja dan kursi, merupakan fasilitas fisik yang penting karena aktivitas belajar siswa, seperti membaca, menggambar, menulis dan kegiatan lainnya, banyak dihabiskan di dalam kelas. Antropometri merupakan faktor penting yang harus dipertimbangkan dalam perancangan perabot kelas guna menentukan dimensi perabot kelas yang tepat dan dapat memfasilitasi pengguna dengan sikap duduk yang baik. Penelitian ini berlokasi di Sekolah Dasar Negeri 060796 Kecamatan Medan Are...

  1. ANALISIS CITRA PERMUKAAN THERMOCHROMIC LIQUID CRYSTAL BERDASARKAN NILAI STATISTIK HUE

    OpenAIRE

    Risti Suryantari; - Flaviana

    2014-01-01

    Tujuan dari penelitian ini adalah untuk mengamati perbedaan citra pada permukaan Thermocromic Liquid Crystal (TLC) R25C5W akibat perubahan temperatur. Metode yang digunakan untuk analisis adalah pengolahan citra berbasis morfologi matematika menggunakan perangkat lunak Matlab2013a. Citra asli yang diperoleh dalam bentuk RGB dikonversi menjadi HSV (hue, saturation, value), dengan mengambil komponen hue saja. Proses utama yang digunakan adalah opening dengan struktur elemen (SE) line. Berdasark...

  2. ANALISIS KUALITAS LULUSAN BERDASARKAN TINGKAT KEPUASAN PENGGUNA LULUSAN

    Directory of Open Access Journals (Sweden)

    Ira Setyaningsih

    2013-06-01

    Full Text Available In education, the students are the products to be input and the output are graduates. Users of graduates are consumers who need to know the level of satisfaction to provide a self-evaluation in improving the institutions. It is also stated in the accreditation forms BAN PT. However, a grain questions that sought to know is very little. The research was conducted at a university in Yogyakarta. Research trying to describe in detail the attributes of quality of graduates based on the user satisfaction level graduates. Object of study is alumni and also alumni users. The study was conducted during the months of August to November 2012. Attributes used are Communication Skills, Organizational Skills, Leadership, Logic, Effort, Ethics, Group and Other Skills. Other attributes are attributes about the ability of areas of expertise. From the results obtained, the attributes that need attention are Organizational Skills and Leadership with the highest gap -0.82. Overall, users of alumni do not feel satisfied, because all negative attributes.

  3. EKUITAS MEREK JASA GERAI BUAH-BUAHAN BERDASARKAN PERSEPSI KONSUMEN

    OpenAIRE

    Erdiasa Nursaman; Ujang Sumarwan; Setiadi Djohar

    2015-01-01

    The need of fruits and the number of fruit outlets in Jakarta has been increasing, therefore the study on brand equity of fruit outlet services according to consumer’s perception is now required. In detail the purpose of this study is to analyze the demographic characteristics of  fruits outlets consumer in Jakarta, analyzing the factors that affect brand equity of fruits outlets services, analyze the level of contribution of brand equity variables fruits outlets service in Jakarta and analyz...

  4. STRATEGI PERBAIKAN - PENGGANTIAN BERDASARKAN UMUR PRODUK UNTUK GARANSI SATU DIMENSI

    Directory of Open Access Journals (Sweden)

    Dyah Ika Rinawati

    2012-02-01

    Full Text Available When the manufacturer selling products with warranties, it causes additional costs (called warranty costs toservice any claims under warranty. Reducing warranty costs is an issue of great interest to manufactures. Thethree ways to reduce warranty costs i.e. improving product reliability, use of preventive maintenance andwarranty servicing strategy.This research deals with a warranty servicing strategy for items sold with a one dimensional warranty where theperiod offered is relatively long. For instance, a computer warranted for three years. For repairable productssold with free repairable warranty (FRW, the manufacturer has the option of either repairing or replacing thefailed item with a new one.In the strategy studied, the decision of repairing or replacing the failed item under warranty is based on its ageat failure. Under the strategy, for failures with age being greater than or equal to threshold parameter will berectified by replacement and all other failures under warranty will be repaired. This strategy is characterized bya threshold parameter which minimizes the expected warranty cost. The optimal parameter is sought by usingnumerical computation. Some numerical examples are given for the purposes of illustration.

  5. Keanekaragaman Mirabilis jalapa L. Berdasarkan Pola Pita Isozim Peroksidase

    Directory of Open Access Journals (Sweden)

    AHMAD DWI SETYAWAN

    2007-05-01

    Full Text Available Mirabilis jalapa L. is a gardening plant which growth easily planted because their beautiful color and flower’s ornaments. The aims of the research were to find out the variation of isozyme band of peroxidase (PER and their close relationship of M. jalapa cultivated based on their different flower’s color. The method used in this study was polyacrilamide gel electrophoresis. The collecting data were analyzed using calculation of association coefficient (Gower’s coefficient with cluster analysis used for similarities of their arithmetic. The results showed that in M. jalapa were found 21 variation of isozyme bands of peroxidase (PER from 8 Rf of 0.04; 0.08; 0.20; 0.28; 0.36; 0.48; 0,60; and 0.64. M. jalapa with white and red nodes flower’s (BIN and white and red line flower’s (GAR have the nearest genetic relationship with similarity index of 0.76. M. jalapa with white and red nodes flower’s (BIN and white and red line flower’s (GAR joined with red flower’s (MER on 0.73 of similarity index then joined with white flower’s (PUT on 0.64 of similarity index and yellow flower’s (KUN on0.49 of similarity index. At least, M. jalapa with orange flower’s (ORA joined with M. jalapa cultivars on 0.41 of similarity index.

  6. PENJABARAN HUKUM ALAM MENURUT PIKIRAN ORANG JAWA BERDASARKAN PRANATA MANGSA

    Directory of Open Access Journals (Sweden)

    Rini Fidiyani

    2012-03-01

    Full Text Available Pranara mangsa is a local knowledge on the management of agricultural land for the Javanese people. This study aimed to determine the ability of Jawva to read the laws of nature and the existence of pranata mangsa on people Banyumas today. What is there in nature is a manifestation of God’s eternal law that by Him manifested in signs of nature. How to interpret the laws of nature by which Java is used as a benchmark in managing land called pranata mangsa.Pranata mangsa are dynamic institution, especially with the uncertain climate change. For farmers Banyumas, pranata mangsa remains a benchmark, but as the development of science and technology, pranata mangsa becoming obsolete. This is a threat to the existence of pranata mangsa as national heritage.

  7. Penentuan Nilai Noise Berdasarkan Slice Thickness Pada Citra CT Scan

    OpenAIRE

    Sihombing, Hediana

    2015-01-01

    Noise measurements have been carried out with changes in thick slices CT Scan image by selecting the ROI on a water phantom objects. The method used in the measurement of noise values ranging from 1 mm, 2 mm, 3 mm, 4 mm, 5 mm, 6 mm, 7 mm, 8 mm, 9 mm, and 10 mm and eksposi factor of 100 kV and 500 mAs. The results obtained are 6.32 HU; 5:14 HU; 3.86 HU; HU 3:48; HU 3:14; 2.94 HU; 2.78 HU; 2.52 HU; HU HU 2:44 and 2:38. The thicker slices diminishing the noise then the resulting CT images wer...

  8. PELESTARIAN KAWASAN PECINAN KEMBANG JEPUN KOTA SURABAYA BERDASARKAN PERSEPSI MASYARAKAT

    OpenAIRE

    Kartika Eka Sari; Antariksa .; Eddi Basuki Kurniawan

    2011-01-01

    This research’s objective are to identify physical, economic and culture characteristic of Kembang Jepun, analyze potention and issue related to Kembang Jepun’s preservation and develop Kembang Jepun’s preservation. Local communities’s perception is use to analyze cultural meaning of Kembang Jepun, especially building’s age, aesthetic, scarcity, originality, strengthen the cultural meaning and area’s image. Based on IPA (Importance Performance Analysis) method, most priority for cultural mean...

  9. PERAMALAN BISNIS BERDASARKAN PERILAKU KONSUMEN MENGGUNAKAN LOGIKA FUZZY

    Directory of Open Access Journals (Sweden)

    Aryanti Aryanti

    2014-02-01

    Full Text Available Perkembangan teknologi informasi sekarang ini telah melahirkan sistem cerdas. Aplikasi dari sistem cerdas dapat digunakan untuk peramalan. Salah satu metode dalam sistem cerdas yang dapat digunakan untuk melakukan peramalan adalah menggunakan logika fuzzy. Penelitian dilakukan dengan memprediksi seberapa jauh keberhasilan bisnis yang akan atau sedang dikerjakan. Sebuah bisnis akan dapat dijalankan dan mampu bertahan dalam era persaingan yang ketat jika perusahaan mampu memperhatikan kepuasan pelanggan atau konsumennya. Perilaku konsumen tidak terlepas dari karakteristik produk baik mengenai penampilan, gaya, mutu dan harga dari produk tersebut. Pada penelitian ini dibangun model regresi menggunakan logika fuzzy antara tingkat pendidikan, pendapatan dan kemampuan membeli seseorang. Model regresi ini diharapkan akan mampu melakukan peramalan tentang bisnis yang akan dijalankan. Pada penelitian ini diperoleh bahwa jika sebagian besar suatu wilayah berpenduduk dengan tingkat pendidikan mahasiswa dan pendapatan sekitar Rp.1 juta maka dapat diduga bahwa keputusan akan pembelian produk sebesar 76.3 atau konsumen akan mampu untuk mengeluarkan dana sebesar 7.63% dari pendapatannya untuk membeli produk. Kata Kunci: Peramalan, Perilaku Konsumen, Logika Fuzzy 

  10. Pembuatan Komposit Termoplastik Berdasarkan Serat Kelapa Sawit Dengan Kaedah Prapreg

    OpenAIRE

    Maulida

    2010-01-01

    The aim of this project to expand the prepreg concept to non non-continuous based fibers, especially agriculture waste and other waste materials like as palm fiber, newspaper fiber and rice husk fiber. Akrylonytrille butadiene styrene (ABS) is used as the matrix, meanwhile methyl ethyl cetone (MEK) acts as the solvent. The scope of the study on the resin mixing technique with known amount with ratio 1 : 1. The resin mixing technique with known amount (method B) produces prepregs with varying ...

  11. EVALUASI PERAN APOTEKER BERDASARKAN PEDOMAN PELAYANAN KEFARMASIAN DI PUSKESMAS

    Directory of Open Access Journals (Sweden)

    Sudibyo Supardi

    2013-02-01

    Full Text Available Abstract The Government Regulation Number 51 on Pharmacy Practice states that dispensing prescriptions in a health facility, including the community health center, must be done by a pharmacist. In 2010 only ten percents of community health centers have a pharmacist. A cross sectional qualitative study has been done to obtain information on the role of pharmacist themselves in practicing in a community health center, and to obtain information on problem and barrier of the role of pharmacist. The study was carried out in Java, namely in cities of Tangerang, Bandung, Surabaya and Bantul District. Informants for in-depth interviews are District/City Health Office staff involved in the pharmacist assignment in a community health center and head of community health center, whereas for focus group discussion we invited pharmacists from District/City Health Office, community health center, school of pharmacy and regional pharmacists association. Descriptive data were analyzed qualitatively using triangulation method and temporary results were reviewed in a round table discussion in Jakarta with DG of Pharmaceutical Services and Medical Devices, Indonesian Pharmacist Association and Provincial Health Office of DKI Jakarta. Results of the study show that: 1. A. pharmacist is not available at all community health centers, as well as non-care community health centers, there are many prescription services performed by non-professional personnel. 2. The role of pharmacist in a community health center covers good drug management just like their job description, especially in prescription dispensing and drug use report. 3. The role of the pharmacist in pharmaceutical services: (a information carried on the delivery of drugs prescription drugs to the patient, prior to clinic services began, and during a visit to posyandu posyandu toddlers and the elderly, (b drug counseling is limited given the availability of time and there is no room , (c visite the patient was done, either with the patient's own doctor or maternity hospitalization, (d home care have not been going well. 4. Issues related to pharmacists in community health centers is the availability and the number does not match to the workload, so that pharmaceutical care have not been going well due to limitations of time and effort. Also there are pharmacists feel less capable in providing drug information to other health professionals, especially medical specialist several health centers, so it is still necessary coaching and training. Keywords: pharmacist, Guideline of pharmaceutical care, community health center, drug management Abstrak Dalam Peraturan Pemerintah RI Nomor 51 tahun 2009 disebutkan pelayanan resep dokter di pelayanan kefarmasian (salah satunya puskesmas harus dilakukan oleh apoteker. Data tahun 2010 menunjukkan hanya 10% puskesmas yang memiliki apoteker. Tujuan penelitian adalah mendapatkan informasi tentang peran apoteker di puskesmas dan permasalahan dalam pelayanan kefarmasian di puskesmas. Hasil penelitian diharapkan sebagai masukan bagi pihak yang terkait untuk meningkatkan ketersediaan apoteker dalam pelayanan kefarmasian di puskesmas. Penelitian potong lintang (cross sectional dengan pendekatan kualitatif dilakukan terhadap instansi yang terkait dengan peran apoteker di puskesmas pada tahun 2011. Lokasi penelitian yang dipilih adalah Provinsi Banten, Jawa Barat, Daerah Istimewa Yogyakarta, dan Jawa Timur. Dari masing-masing provinsi diambil satu kota, yaitu Kota Tangerang, Kota Bandung, Kabupaten Bantul dan Kota Surabaya. Informan penelitian untuk wawancara mendalam adalah Dinkes Kabupaten/Kota dan Kepala Puskesmas, sedangkan peserta diskusi kelompok terarah adalah 12 apoteker yang mewakili Dinkes Kabupaten/Kota, puskesmas perawatan, Perguruan Tinggi Farmasi (PTF dan Pengurus Daerah Ikatan Apoteker Indonesia (PD IAI. Analisis data secara deskriptif kualitatif dengan metoda triangulasi sumber data dan triangulasi metoda pengumpulan data. Hasil sementara disempurnakan dengan Round Table Discussion di Jakarta dengan mengundang nara sumber.

  12. Khazana Warna Berdasarkan Hasana Alam dan Budaya Nusantara (1)

    OpenAIRE

    Mita Purbasari Wahidiyat; R. A. Diah Resita I. K. Jakti

    2011-01-01

    Using colors for designing purpose is become an important part of designer, such as graphic designer, interior, product, least but not last for architect and artist. Like humans, color need to have a name to identify according to its character, psychology and emotional effect. This research applied the qualitative and quantitative method to give a brief description about colors name and propose new color naming which based on character, nature, and imagery it evokes. This research...

  13. ANALISIS KUALITAS LULUSAN BERDASARKAN TINGKAT KEPUASAN PENGGUNA LULUSAN

    OpenAIRE

    Ira Setyaningsih; Muchammad Abrori

    2013-01-01

    In education, the students are the products to be input and the output are graduates. Users of graduates are consumers who need to know the level of satisfaction to provide a self-evaluation in improving the institutions. It is also stated in the accreditation forms BAN PT. However, a grain questions that sought to know is very little. The research was conducted at a university in Yogyakarta. Research trying to describe in detail the attributes of quality of graduates based on the user satisf...

  14. ALOKASI PASOKAN BERDASARKAN PRODUK UNGGULAN UNTUK RANTAI PASOK SAYURAN SEGAR

    Directory of Open Access Journals (Sweden)

    Marimin Marimin

    2007-01-01

    Full Text Available Application of artificial inteligent by using fuzzy logic can be one of way to handle supply chain situation in uncertainty environment. It was widely concern to study a supply chain using fuzzy logic that most needed. Ain of the study was apply fuzzy logic in determination of supply allocation for superior product in vegetables supply chain. Models was developed in three sub models. First was determination vegetables types that superior by using pareto technique and exponential compaarison method (MPE. Second was expert system to determine supply needed and finally was optimizing supply allocation using fuzzy multi-objective linear programming. The model was applied in a vegetables agroindustry that superior product selected was red paprica. Analysis againts model behaviour is conducted for pesimistic and optimistic scenarios. Abstract in Bahasa Indonesia : Penerapan kecerdasan buatan dengan logika fuzzy bisa menjadi salah satu cara untuk mengatasi situasi rantai pasok dalam lingkungan ketidakpastian. Perhatian yang lebih luas untuk pembahasan rantai pasok menggunakan teknik fuzzy masih sangat dibutuhkan. Studi ini bertujuan menerapkan logika fuzzy dalam penentuan alokasi pasokan untuk produk unggulan pada rantai pasok agroindustri sayuran. Model yang terdiri dari tiga sub model. Sub model pertama adalah penentuan jenis sayuran yang diunggulkan menggunakan kombinasi teknik pareto dan metode perbandingan eksponensial (MPE. Sub model kedua adalah sistem pakar untuk menentukan kebutuhan pasokan menggunakan logika fuzzy. Sub model ketiga adalah optimasi alokasi pasokan menggunakan programa linear obyektif majemuk fuzzy. Model yang dibangun kemudian diterapkan pada sebuah perusahaan agroindustri sayuran dengan produk unggulan terpilih adalah paprika merah. Analisis terhadap prilaku model juga dilakukan untuk skenario pesimis dan optimis. Kata kunci: fuzzy, sistem pakar, MPE, obyektif majemuk, sayuran

  15. Penentuan Konsentrasi Tanah dalam Air Sungai Berdasarkan Perbedaan Warna

    Directory of Open Access Journals (Sweden)

    Imam Sofi'i

    2008-08-01

    Full Text Available Information of soil erosion is very useful related with prevention action and planning in the future. The objective of this research is to determine soil concentration in water river based on colour differences using image processing and artificial neural network (ANN. Soil and water mixture image was taken using a digital camera then was processed by an image processing program. Two ANN Models were developed. The first model had 3 input parameters while the second model had 6 input parameters. Both models had altogether one output parameters of the soil concentration. The accuracy of the first model was 38% while the second one was 36%.

  16. PEMETAAN PREFERENSI KONSUMEN SUPERMARKET DENGAN METODE KANO BERDASARKAN DIMENSI SERVQUAL

    OpenAIRE

    Kriswanto Widiawan; Irianty Irianty

    2004-01-01

    The management of supermarket usually has some assumptions about the customers' expectation. Sometimes these assumtions do not reflect the nature of customers' expectations. Because of this miss perception, the supermarket looses its profit and becomes inefficient. This research was carried out in order to find out the customers' expectation on facilities and services of the supermarkets according to the servqual dimensions. The outcomes will be mapped into Kano categorizations. The researche...

  17. The influence of surface structure on H4SiO4 oligomerization on rutile and amorphous TiO2 surfaces: an ATR-IR and synchrotron XPS study.

    Science.gov (United States)

    Song, Yantao; Swedlund, Peter J; McIntosh, Grant J; Cowie, Bruce C C; Waterhouse, Geoffrey I N; Metson, James B

    2012-12-11

    Silicic acid (H(4)SiO(4)) is ubiquitous in natural aquatic systems. Applications of TiO(2) in these systems will be influenced by H(4)SiO(4) sorption and oligomerization reactions on the TiO(2) surface, and this can affect many aspects of TiO(2) reactivity. The spatial arrangement of sorption sites on a metal oxide surface can promote specific lateral interactions, such as oligomerization, between sorbed species. In this work we explore the relationship between surface structure and interfacial H(4)SiO(4) oligomerization by quantifying the extent of H(4)SiO(4) sorption and oligomerization on three TiO(2) phases; a rutile phase having well-developed (110) faces (R180), a rutile phase with poorly developed (110) faces (R60), and an amorphous TiO(2) (TiO(2(am))). The in situ ATR-IR spectra measured over time as 0.2 mM H(4)SiO(4) reacted with TiO(2) were quite different on the three TiO(2) phases. The percentage of the surface H(4)SiO(4) that was present as oligomers increased over time on all phases, but after 20 h almost all H(4)SiO(4) on the R180 surface was oligomeric, while the H(4)SiO(4) on TiO(2(am)) was predominantly monomeric. The extent of H(4)SiO(4) oligomerization on R60 was intermediate. When the TiO(2) phases reacted with 1.5 mM H(4)SiO(4) the ATR-IR spectra showed oligomeric silicates dominating the surface of all three TiO(2) phases; however, after 20 h the percentage of the surface H(4)SiO(4) present as three-dimensional polymers was ∼30, 10, and 0% on R180, R60, and TiO(2(am)) respectively. The Si 2s photoelectron peak binding energy (BE) and the H(4)SiO(4) surface coverage (Γ(Si)) were measured by XPS over a range of Γ(Si). For any given Γ(Si) the Si 2s BE's were in the order R180 > R60 > TiO(2(am)). A higher Si 2s BE indicates a greater degree of silicate polymerization. The ATR-IR and XPS results support the existing model for interfacial H(4)SiO(4) oligomerization where linear trimeric silicates are formed by insertion of a solution H(4)SiO(4

  18. Using ATR-FT/IR to detect carbohydrate-related molecular structure features of carinata meal and their in situ residues of ruminal fermentation in comparison with canola meal

    Science.gov (United States)

    Xin, Hangshu; Yu, Peiqiang

    2013-10-01

    There is no information on the co-products from carinata bio-fuel and bio-oil processing (carinata meal) in molecular structural profiles mainly related to carbohydrate biopolymers in relation to ruminant nutrition. Molecular analyses with Fourier transform infrared spectroscopy (FT/IR) technique with attenuated total reflectance (ATR) and chemometrics enable to detect structural features on a molecular basis. The objectives of this study were to: (1) determine carbohydrate conformation spectral features in original carinata meal, co-products from bio-fuel/bio-oil processing; and (2) investigate differences in carbohydrate molecular composition and functional group spectral intensities after in situ ruminal fermentation at 0, 12, 24 and 48 h compared to canola meal as a reference. The molecular spectroscopic parameters of carbohydrate profiles detected were structural carbohydrates (STCHO, mainly associated with hemi-cellulosic and cellulosic compounds; region and baseline ca. 1483-1184 cm-1), cellulosic compounds (CELC, region and baseline ca. 1304-1184 cm-1), total carbohydrates (CHO, region and baseline ca. 1193-889 cm-1) as well as the spectral ratios calculated based on respective spectral intensity data. The results showed that the spectral profiles of carinata meal were significantly different from that of canola meal in CHO 2nd peak area (center at ca. 1091 cm-1, region: 1102-1083 cm-1) and functional group peak intensity ratios such as STCHO 1st peak (ca. 1415 cm-1) to 2nd peak (ca. 1374 cm-1) height ratio, CHO 1st peak (ca. 1149 cm-1) to 3rd peak (ca. 1032 cm-1) height ratio, CELC to total CHO area ratio and STCHO to CELC area ratio, indicating that carinata meal may not in full accord with canola meal in carbohydrate utilization and availability in ruminants. Carbohydrate conformation and spectral features were changed by significant interaction of meal type and incubation time and almost all the spectral parameters were significantly decreased (P meal

  19. Using ATR-FT/IR to detect carbohydrate-related molecular structure features of carinata meal and their in situ residues of ruminal fermentation in comparison with canola meal

    Science.gov (United States)

    Xin, Hangshu; Yu, Peiqiang

    2013-10-01

    There is no information on the co-products from carinata bio-fuel and bio-oil processing (carinata meal) in molecular structural profiles mainly related to carbohydrate biopolymers in relation to ruminant nutrition. Molecular analyses with Fourier transform infrared spectroscopy (FT/IR) technique with attenuated total reflectance (ATR) and chemometrics enable to detect structural features on a molecular basis. The objectives of this study were to: (1) determine carbohydrate conformation spectral features in original carinata meal, co-products from bio-fuel/bio-oil processing; and (2) investigate differences in carbohydrate molecular composition and functional group spectral intensities after in situ ruminal fermentation at 0, 12, 24 and 48 h compared to canola meal as a reference. The molecular spectroscopic parameters of carbohydrate profiles detected were structural carbohydrates (STCHO, mainly associated with hemi-cellulosic and cellulosic compounds; region and baseline ca. 1483-1184 cm-1), cellulosic compounds (CELC, region and baseline ca. 1304-1184 cm-1), total carbohydrates (CHO, region and baseline ca. 1193-889 cm-1) as well as the spectral ratios calculated based on respective spectral intensity data. The results showed that the spectral profiles of carinata meal were significantly different from that of canola meal in CHO 2nd peak area (center at ca. 1091 cm-1, region: 1102-1083 cm-1) and functional group peak intensity ratios such as STCHO 1st peak (ca. 1415 cm-1) to 2nd peak (ca. 1374 cm-1) height ratio, CHO 1st peak (ca. 1149 cm-1) to 3rd peak (ca. 1032 cm-1) height ratio, CELC to total CHO area ratio and STCHO to CELC area ratio, indicating that carinata meal may not in full accord with canola meal in carbohydrate utilization and availability in ruminants. Carbohydrate conformation and spectral features were changed by significant interaction of meal type and incubation time and almost all the spectral parameters were significantly decreased (P

  20. De El cuarto de atrás de Carmen Martín Gaite a La meitat de l’ànima de Carme Riera: Notas sobre la memoria histórica en la novela contemporánea / From Carmen Martín Gaite’s El cuarto de atrás to Carme Riera’s La meitat de l’ànima: Some notes on historical memory in contemporary fiction

    Directory of Open Access Journals (Sweden)

    Mario Santana

    2011-01-01

    Full Text Available Resumen: La novela española contemporánea ha dado una relevancia especial al interés por recuperar y transmitir la memoria colectiva del pasado reciente. Si bien la denominada “novela de memoria” (HERZBERGER, 1995 ya es una constante en la producción narrativa desde los primeros años de la transición a la democracia, este ensayo propone que en la última década ha ido ganando popularidad una “novela de la memoria histórica” con rasgos estructurales y temáticos que la convierten en una modalidad diferenciada. A partir del concepto de memoria heteropática (SILVERMAN, 1996 y del estudio de las semejanzas y diferencias entre dos novelas paradigmáticas de uno y otro modelo narrativo —El cuarto de atrás de Carmen Martín Gaite (1978 y La meitat de l’ànima de Carme Riera (2004, respectivamente—, se identifican algunos de los rasgos más distintivos de la novela de la memoria histórica y en especial su articulación literaria de la memoria como proyecto colectivo y ciudadano.Summary: The recovery and transmission of collective memory about the recent past plays an important role in contemporary Spanish fiction. While the production of “novels of memory” (HERZBERGER, 1995 has been consistent since the beginning of the transition to democracy, this essay proposes that in the last decade has gained popularity a “novel of historical memory”, which has distinctive estructural and thematic features and deserves to be studied as a differentiated modality. Using as a point of departure the notion of heteropathic memory (SILVERMAN, 1996 and through the analysis of the parallels and differences between two novels representative of both models —Carmen Martín Gaite’s El cuarto de atrás (1978 and Carme Riera’s La meitat de l’ànima (2004, respectively—, the essay identifies some of the most distinctive features of the novel of historical memory and, in particular, the literary articulation of memory as a collective and

  1. Detect changes in protein structure of carinata meal during rumen fermentation in relation to basic chemical profile and comparison with canola meal using ATR-FT/IR molecular spectroscopy with chemometrics

    Science.gov (United States)

    Xin, Hangshu; Yu, Peiqiang

    2013-08-01

    As far as we know, no study has been carried out on whether protein structure changes in the feed during rumen fermentation from other research team. This study was conducted to characterize protein structure spectral changes in carinata meal during ruminal fermentation using Fourier transform infrared spectroscopy (FT/IR) technique with ATR. The objectives were to find out whether (1) protein internal structure (in terms of protein amide profile and protein secondary structure profile) changed after in situ ruminal fermentation at 0, 12, 24 and 48 h in carinata meal and conventional canola meal was used as a reference; (2) there was any correlation between protein spectral parameters and basic chemical profile in in situ rumen residue samples; and (3) the protein structural chemical make-up of carinata meal differed from canola meal during 48 h rumen incubation. The results showed that protein structure features in both carinata meal and canola meal were altered as incubation time increased (P meal was not distinguished from those from canola meal, suggesting some relationship in structural make-up exhibited between them within protein region during 48 h rumen fermentation. Further studies are still needed to investigate detailed information on structural changes in protein of various feedstuffs in order to fully and deeply understand protein degradation during rumen fermentation on both metabolic basis and molecular biological basis.

  2. Effects of arsenic trioxide on ATM/ATR gene expression and cell apoptosis in K562 cells%As2O3对K562 ATM/ATR基因表达和细胞凋亡的影响

    Institute of Scientific and Technical Information of China (English)

    叶敏军; 任行洲

    2008-01-01

    目的 探讨不同浓度As2O3对K562细胞ATM(Ataxia Telangiectasia Mutated)/ATR(ATM-Rad3-Related)基因表达的影响及相应的细胞周期变化和细胞凋亡机制.方法 以不同剂量的As2O3作用于K562细胞株,RT-PCR半定量检测ATM/ATR基因表达,流式细胞术检测P53、Annexin V和细胞周期.结果 随药物浓度升高,ATM基因表达量增强而ATR基因表达量降低,P53水平升高,细胞凋亡显著,亚二倍体和G1期细胞数增多,S期和G2/M期细胞减少.不加入药物的K562细胞ATM和ATR几乎不表达.结论 ATM和ATR基因表达变化呈As203浓度依赖的形式,且与P53水平、细胞周期和细胞凋亡率的改变相关.

  3. Estimativa dos índices de pneumonia, pela tosse, e de rinite atrófica, por espirros, em suínos Estimating of pneumonia by coughing and atrophic rhinitis by sneezing indices in swine

    Directory of Open Access Journals (Sweden)

    N. Morés

    2001-06-01

    Full Text Available Realizou-se um estudo em 64 granjas de suínos na região sul do Brasil entre julho de 1995 e março de 1997 com o objetivo de estimar o índice de pneumonia (IP e o índice de rinite atrófica (IRA, por meio da contagem dos sinais clínicos de tosse e espirro, respectivamente, em suínos em crescimento-terminação. Em cada granja, um lote com cerca de 60 suínos foi acompanhado desde o alojamento na fase de crescimento até o abate. Tosse e espirro foram contados em quatro oportunidades (30, 50 e 80 dias após o alojamento e 1 a 3 dias antes do abate. Em cada oportunidade foram feitas três contagens de dois minutos cada e o percentual de tosse e espirro para cada lote foi calculado pela média das três contagens em relação ao tamanho do lote. No abate, os suínos foram avaliados quanto à freqüência e severidade de lesões de hepatização pulmonar e de rinite atrófica nos cornetos nasais para cálculo do IP e do IRA, respectivamente. Os dados foram submetidos às análises de correlação de Pearson e de regressão simples para ajuste da equação de predição do IP em relação à porcentagem de tosse e do IRA em relação à porcentagem de espirro. As equações obtidas foram: IPe = 0,35 + (0,11 × % de tosse com R² = 0,45 e IRAe = 0,36 +( 0,065 × % de espirro com R² = 0,36. Conclui-se que é possível utilizar o método de contagem de tosse e espirro na fase de crescimento-terminação para estimar, respectivamente, o IP e o IRA.A study in 64 swine herds was carried out in order to estimate the pneumonia index (PI and the atrophic rhinitis index (ARI through the quantification of coughing and sneezing in growing-finishing pigs. In each herd, 60 pigs were evaluated from lodging in the growing-finishing phase up to slaughter. The amount of coughing and sneezing episodes was counted in four opportunities (30, 50 and 80 days after lodging, and three days before slaughter. In each opportunity three counts of two minutes each were

  4. Direct determination of sorbitol and sodium glutamate by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) in the thermostabilizer employed in the production of yellow-fever vaccine.

    Science.gov (United States)

    de Castro, Eduardo da S G; Cassella, Ricardo J

    2016-05-15

    Reference methods for quality control of vaccines usually require treatment of the samples before analysis. These procedures are expensive, time-consuming, unhealthy and require careful manipulation of the sample, making them a potential source of analytical errors. This work proposes a novel method for the quality control of thermostabilizer samples of the yellow fever vaccine employing attenuated total reflectance Fourier transform infrared spectrometry (ATR-FTIR). The main advantage of the proposed method is the possibility of direct determination of the analytes (sodium glutamate and sorbitol) without any pretreatment of the samples. Operational parameters of the FTIR technique, such as the number of accumulated scans and nominal resolution, were evaluated. The best conditions for sodium glutamate were achieved when 64 scans were accumulated using a nominal resolution of 4 cm(-1). The measurements for sodium glutamate were performed at 1347 cm(-1) (baseline correction between 1322 and 1369 cm(-1)). In the case of sorbitol, the measurements were done at 890cm(-1) (baseline correction between 825 and 910 cm(-1)) using a nominal resolution of 2 cm(-1) with 32 accumulated scans. In both cases, the quantitative variable was the band height. Recovery tests were performed in order to evaluate the accuracy of the method and recovery percentages in the range 93-106% were obtained. Also, the methods were compared with reference methods and no statistical differences were observed. The limits of detection and quantification for sodium glutamate were 0.20 and 0.62% (m/v), respectively, whereas for sorbitol they were 1 and 3.3% (m/v), respectively.

  5. Direct determination of sorbitol and sodium glutamate by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) in the thermostabilizer employed in the production of yellow-fever vaccine.

    Science.gov (United States)

    de Castro, Eduardo da S G; Cassella, Ricardo J

    2016-05-15

    Reference methods for quality control of vaccines usually require treatment of the samples before analysis. These procedures are expensive, time-consuming, unhealthy and require careful manipulation of the sample, making them a potential source of analytical errors. This work proposes a novel method for the quality control of thermostabilizer samples of the yellow fever vaccine employing attenuated total reflectance Fourier transform infrared spectrometry (ATR-FTIR). The main advantage of the proposed method is the possibility of direct determination of the analytes (sodium glutamate and sorbitol) without any pretreatment of the samples. Operational parameters of the FTIR technique, such as the number of accumulated scans and nominal resolution, were evaluated. The best conditions for sodium glutamate were achieved when 64 scans were accumulated using a nominal resolution of 4 cm(-1). The measurements for sodium glutamate were performed at 1347 cm(-1) (baseline correction between 1322 and 1369 cm(-1)). In the case of sorbitol, the measurements were done at 890cm(-1) (baseline correction between 825 and 910 cm(-1)) using a nominal resolution of 2 cm(-1) with 32 accumulated scans. In both cases, the quantitative variable was the band height. Recovery tests were performed in order to evaluate the accuracy of the method and recovery percentages in the range 93-106% were obtained. Also, the methods were compared with reference methods and no statistical differences were observed. The limits of detection and quantification for sodium glutamate were 0.20 and 0.62% (m/v), respectively, whereas for sorbitol they were 1 and 3.3% (m/v), respectively. PMID:26992492

  6. 中国旅游研究国际刊文分析——以ATR、TM和JTR为例%An Analysis of China's Tourism Research Published on International Journals:Taking ATR, TM and JTR for Examples

    Institute of Scientific and Technical Information of China (English)

    李经龙; 徐玉梅

    2015-01-01

    通过对Annals of Tourism Research (ATR)、Tourism Management (TM)、Journal of Travel Research (JTR)这三大国际权威旅游期刊创刊以来刊文的中国学者信息进行统计和分析,进而洞察我国旅游研究的历程、我国旅游研究实力和旅游学术共同体的区域差异以及我国旅游研究学者的个体差异,并借助ROSTCM6.0软件对我国学者刊文的题目进行社会网络语义分析,最后分别对我国学者在三大期刊发文进行统计分析.结果表明:我国旅游研究具有明显的阶段性,可以大致分为探索、参与、发展和壮大四个阶段;中国台湾是我国旅游研究实力最强的地区;香港理工大学是我国旅游研究实力最雄厚的学府;Bob McKercher、Ching-Fu Chen、Haiyan Song和Cathy H.C.Hsu是我国在三大期刊刊文比较多的学者,都在8篇以上;而且我国学者关注的内容也很广泛,涉及了旅游领域的各个方面.

  7. In vitro evaluation of ionizing radiation effects in bone tissue by FTIR spectroscopy and dynamic mechanical analysis; Avaliacao in vitro dos efeitos da radiacao ionizante em tecido osseo bovino por espectroscoia ATR-FTIR e analise dinamica-mecanica

    Energy Technology Data Exchange (ETDEWEB)

    Veloso, Marcelo Noronha

    2013-07-01

    Ionizing radiation from gamma radiation sources or X-ray generators is frequently used in Medical Science, such as radiodiagnostic exams, radiotherapy, and sterilization of haloenxerts. Ionizing radiation is capable of breaking polypeptidic chains and causing the release of free radicals by radiolysis.of water. It interacts also with organic material at the molecular level, and it may change its mechanical properties. In the specific case of bone tissue, studies report that ionizing radiation induces changes in collagen molecules and reduces the density of intermolecular crosslinks. The aim of this study was to verify the changes promoted by different doses of ionizing radiation in bone tissue using Fourier Transform Infrared Spectroscopy (FTIR) and dynamic mechanical analysis (DMA). Samples of bovine bone were irradiated using Cobalt-60 with five different doses: 0.01 kGy, 0.1 kGy, 1 kGy, 15 kGy and 75 kGy. To study the effects of ionizing irradiation on the chemical structure of the bone, the sub-bands of amide I, the crystallinity index and relation of organic and inorganic materials, were studied. The mechanical changes were evaluated using the elastic modulus and the damping value. To verify whether the chemical changes and the mechanical characteristics of the bone were correlated, the relation between the analysis made with spectroscopic data and the mechanical analysis data was studied. It was possible to evaluate the effects of different doses of ionizing radiation in bone tissue. With ATR-FTIR spectroscopy, it was possible to observe changes in the organic components and in the hydroxyapatite crystals organization. Changes were also observed in the elastic modulus and in the damping value. High correlation with statistical significance was observed among (amide III + collagen)/{sub v1,v3}, PO{sub 4}{sup 3-} and the delta tangent, and among 1/FHWM and the elastic modulus. (author)

  8. The influence of ATM, ATR, DNA-PK inhibitors on the cytotoxic and genotoxic effects of dibenzo[def,p]chrysene on human hepatocellular cancer cell line HepG2.

    Science.gov (United States)

    Spryszyńska, Sylwia; Smok-Pieniążek, Anna; Ferlińska, Magdalena; Roszak, Joanna; Nocuń, Marek; Stępnik, Maciej

    2015-09-01

    The effect of inhibitors of phosphatidylinositol-3-kinase related kinases (PIKK): ataxia-telangiectasia mutated (ATM), ATM- and Rad3-related (ATR) and DNA-dependent protein kinase (DNA-PK) on the response of HepG2 human liver cancer cells to dibenzo[def,p]chrysene (DBC) was investigated. High cytotoxicity of DBC (IC50=0.1μM) was observed after 72h incubation. PIKK inhibitors: KU55933 (5μM), NU7026 (10μM) or caffeine (1 and 2mM) when used alone did not significantly influence the cytotoxicity. However, two combinations: KU55933/NU7026 and caffeine/NU7026 significantly increased HepG2 viability (by 25%) after treatment with DBC at 0.5μM. The cytoprotective effect was confirmed by cell cycle and apoptosis/necrosis analysis. DNA damage level after exposure to DBC assessed by comet assay (single strand breaks) showed a long persistence and significant decrease after incubation of the cells in the presence the inhibitors (the combination of KU55933+NU7026 showed the strongest effect). Weak induction of reactive oxygen species (ROS) by DBC (0.5μM) was observed. Although, KU55933 and NU7026 when used alone did not increase ROS levels in the cells, their combination induced the ROS increase and moderately enhanced ROS generation by DBC. We propose a mechanism how cells with damaged DNA after exposure to DBC and under the condition of PIKK inhibition, may be at higher risk of undergoing malignant transformation. PMID:26338538

  9. Alternativas a la elevación de seno maxilar: rehabilitación del sector posterior del maxilar atrófico mediante implantes pterigoideos Alternatives to maxillary sinus lift: posterior area of the atrophic maxilla rehabilitation by means pterigoideal implants

    Directory of Open Access Journals (Sweden)

    X. Rodríguez-Ciurana

    2008-12-01

    Full Text Available El primer y segundo molar son los dientes que se pierden más a menudo debido a la enfermedad periodontal y al exceso de fuerzas oclusales. A pesar de la frecuencia del edentulismo parcial del sector posterior raramente se ubican los implantes más allá de los premolares debido al aumento del índice de fallos. El escaso volumen óseo y la pobre mineralización del sector posterior del maxilar comprometen la viabilidad de las rehabilitaciones con implantes a largo plazo. Además, la cresta ósea a nivel del seno maxilar, en el maxilar atrófico, no suele permitir el anclaje de implantes de 10 mm. Se han propuesto varias técnicas para rehabilitar el sector posterior del maxilar atrófico: implantes cortos, extensiones protésicas, injerto sinusal, implante cigomático. El implante pterigoideo es otro de los posibles tratamientos para rehabilitar el sector posterior del maxilar atrófico. Anclado en el hueso cortical de la apófisis del esfenoides el implante pterigoideo evita la necesidad de injertar o utilizar extensiones protésicas. El objetivo de este artículo es analizar las indicaciones, la técnica, complicaciones y supervivencia del implante pterigoideo en el sector posterior del maxilar atrófico.The first and second molar are the teeth most commonly lost in the maxilla, mainly due to periodontal disease and excessive occlusal force. 1 Although partial edentulism of the posterior maxilla is common, implants are seldom placed distal to the premolars because failure rates in the posterior maxilla have historically been high. Poor volume and low density of bone are the worst conditions for long-term anchorage in the maxilla. 7 Moreover, bone under the maxillary sinus, in the atrophic maxilla, is usually insufficient to enable placement of 10 mm implants. Several techniques have been proposed to restore the atrophic posterior maxilla: short implants, prosthetic cantilevers, sinus bone graft, zigomatic fixtures. Pterygoid implants are

  10. SAR ATR Based on Convolutional Neural Network

    OpenAIRE

    Tian Zhuangzhuang; Zhan Ronghui; Hu Jiemin; Zhang Jun

    2016-01-01

    This study presents a new method of Synthetic Aperture Radar (SAR) image target recognition based on a convolutional neural network. First, we introduce a class separability measure into the cost function to improve this network’s ability to distinguish between categories. Then, we extract SAR image features using the improved convolutional neural network and classify these features using a support vector machine. Experimental results using moving and stationary target acquisition and recogni...

  11. SAR ATR Based on Convolutional Neural Network

    Directory of Open Access Journals (Sweden)

    Tian Zhuangzhuang

    2016-06-01

    Full Text Available This study presents a new method of Synthetic Aperture Radar (SAR image target recognition based on a convolutional neural network. First, we introduce a class separability measure into the cost function to improve this network’s ability to distinguish between categories. Then, we extract SAR image features using the improved convolutional neural network and classify these features using a support vector machine. Experimental results using moving and stationary target acquisition and recognition SAR datasets prove the validity of this method.

  12. Applying target shadow models for SAR ATR

    Science.gov (United States)

    Papson, Scott; Narayanan, Ram M.

    2007-04-01

    Recent work has suggested that target shadows in synthetic aperture radar (SAR) images can be used effectively to aid in target classification. The method outlined in this paper has four steps - segmentation, representation, modeling, and selection. Segmentation is the process by which a smooth, background-free representation of the target's shadow is extracted from an image chip. A chain code technique is then used to represent the shadow boundary. Hidden Markov modeling is applied to sets of chain codes for multiple targets to create a suitable bank of target representations. Finally, an ensemble framework is proposed for classification. The proposed model selection process searches for an optimal ensemble of models based on various target model configurations. A five target subset of the MSTAR database is used for testing. Since the shadow is a back-projection of the target profile, some aspect angles will contain more discriminatory information then others. Therefore, performance is investigated as a function of aspect angle. Additionally, the case of multiple target looks is considered. The capability of the shadow-only classifier to enhance more traditional classification techniques is examined.

  13. Notes on the 2015 ATRS World Conference

    OpenAIRE

    KİRACI, Kasım

    2015-01-01

    Abstract. In this study, the evaluation of the 19.The ATSR (The Air Transport Research Society) World Conference held between 2-5 July 2015 in Singapore will be mentioned.Keywords. Civil Aviation, World Conference, Singapore.JEL. L62, L93.

  14. Rapid Isolation of Phenol Degrading Bacteria by Fourier Transform Inf rared (FTIR) Spectroscopy%利用衰减全反射傅里叶红外光谱(ATR-FTIR)技术快速筛选可降解苯酚菌株

    Institute of Scientific and Technical Information of China (English)

    李霏; 宋文军; 魏纪平; 王素英; 刘崇基

    2015-01-01

    Phenol is an important chemical engineering material and ubiquitous in industry wastewater ,its ex-istence has become a thorny issue in many developed and developing country .More and more stringent stand-ards for effluent all over the world with human realizing the toxicity of phenol have been announced .Many ad-vanced biological methods are applied to industrial wastewater treatment with low cost ,high efficiency and no secondary pollution ,but the screening of function microorganisms is certain cumbersome process .In our study a rapid procedure devised for screening bacteria on solid medium can degrade phenol coupled with attenuated total reflection fourier transform infrared (ATR-FTIR) which is a detection method has the characteristics of efficient ,fast ,high fingerprint were used .Principal component analysis (PCA) is a method in common use to extract fingerprint peaks effectively ,it couples with partial least squares (PLS) statistical method could estab-lish a credible model .The model we created using PCA-PLS can reach 99.5% of coefficient determination and validation data get 99.4% ,which shows the promising fitness and forecasting of the model .The high fitting model is used for predicting the concentration of phenol at solid medium where the bacteria were grown .The highly consistent result of two screening methods ,solid cultural with ATR-FTIR detected and traditional liq-uid cultural detected by GC methods ,suggests the former can rapid isolate the bacteria which can degrade sub-strates as well as traditional cumbersome liquid cultural method .Many hazardous substrates widely existed in industry wastewater ,most of them has specialize fingerprint peaks detected by ATR-FTIR ,thereby this detec-ted method could be used as a rapid detection for isolation of functional microorganisms those can degrade many other toxic substrates .%苯酚是一种重要的化工原料并广泛存在于工业废水中,随着各国对苯酚生物毒性的认识,排放

  15. 衰减全反射红外光谱法快速分类和识别多种食用油的方法%Research on Rapid Discrimination of Edible Oil by ATR Infrared Spectroscopy

    Institute of Scientific and Technical Information of China (English)

    马啸; 袁洪福; 宋春风; 胡爱琴; 李效玉; 赵众; 李秀琴; 国振; 朱志强

    2015-01-01

    A rapid discrimination method of edible oils ,KL‐BP model ,was proposed by attenuated total reflectance infrared spectroscopy .The model extracts the characteristic of classification from source data by KL and reduces data dimension at the same time .Then the neural network model is constructed by the new data which as the input of the model .84 edible oil samples which include sesame oil ,corn oil ,canola oil ,blend oil ,sunflower oil ,peanut oil ,olive oil ,soybean oil and tea seed oil ,were collected and their infrared spectra determined using an ATR FT‐IR spectrometer .In order to compare the method performance , principal component analysis (PCA) direct‐classification model ,KL direct‐classification model ,PLS‐DA model ,PCA‐BP model and KL‐BP model are constructed in this paper .The results show that the recognition rates of PCA ,PCA‐BP ,KL ,PLS‐DA and KL‐BP are 59.1% ,68.2% ,77.3% ,77.3% and 90.9% for discriminating the 9 kinds of edible oils ,respectively .KL extracts the eigenvector which make the distance between different class and distance of every class ratio is the largest .So the method can get much more classify information than PCA .BP neural network can effectively enhance the classification ability and accuracy . Taking full of the advantages of KL in extracting more category information in dimension reducing and the features of BP neural network in self‐learning ,adaptive ,nonlinear ,the KL‐BP method has the best classification ability and recognition accuracy and great importance for rapidly recognizing edible oil in practice .%提出了一种衰减全反射红外光谱法快速分类和识别多种食用油的方法———KL‐BP模型。此模型利用KL算法对原始光谱数据分类特征进行提取并对原始数据降维,降维后的数据作为神经网络的输入建立分析模型。实验共收集了九种食用油包括芝麻油、玉米油、油菜籽油、调和油、葵花油、花生油、橄榄油

  16. 二维衰减全反射红外光谱研究水在聚酰亚胺/二氧化硅纳米高分子复合膜中的吸附扩散行为%Two-dimensional ATR-FTIR Spectroscopic Study on the Water Diffusion Behavior in Polyimide/Silica Nanocomposite

    Institute of Scientific and Technical Information of China (English)

    沈怡; 王海涛; 钟伟; 武培怡

    2006-01-01

    考虑到基于聚酰亚胺衍生物的电子器件的稳定性和性能,运用二维衰减全反射红外相关光谱研究了水在聚酰亚胺衍生物:poly(4'4-oxydiphenylene pyromellitimide)和二氧化硅的纳米高分子复合膜中的动态吸附和扩散行为.二维相关光谱区分出了三种不同氢键强度的水分子状态,同时,氢键的数量和强度还对不同状态水分子的扩散速率起了决定性的作用,和PI聚合物本身以及PI表面残留的硅酸形成氢键的水分子的扩散速度则最慢.%To consider the reliability and performance of electronic devices based on polyimide derivatives, dynamic water sorption and diffusion behavior in a polyimide derivative: poly(4'4-oxydiphenylene pyromellitimide)(PMDA-ODA)/silica nanocomposite was investigated by two-dimensional ATR-FTIR spectroscopy, by which three states of water molecules owning different H-bonding strength were distinguished. The amounts and strength of H-bonding also played a significant role in determining the diffusion rate of the different states of water molecules. The type of aggregated water molecules which also formed H-bonding with silicic acid (residues) or polyimide system was the last one diffusing to the polymer side in contact with the ATR crystal element because the polymeric matrix blocked their diffusion to a great extent. The diffusion coefficient was also estimated to gain the information of the dynamic diffusion behavior.

  17. Cirurgia laparoscópica no diagnóstico de gastrite crônica atrófica seguida de tratamento clínico em cadela: relato de caso Laparoscopic surgery in the diagnosis of atrophic gastritis in a dog followed by clinical treatment: case report

    Directory of Open Access Journals (Sweden)

    P.C. Basso

    2007-10-01

    Full Text Available Descrevem-se o diagnóstico e o tratamento de um caso de gastrite atrófica crônica, em uma cadela sem raça definida, de dois anos de idade. A paciente apresentava como principal sintomatologia vômito crônico. O hemograma, a urinálise e as avaliações bioquímicas séricas não revelaram alterações significativas. Os exames radiológicos e ultra-sonográficos abdominais também não foram sugestivos de alterações. Realizaram-se inspeção da cavidade peritoneal, gastrotomia, gastroscopia, gastrectomia para biopsia e gastrorrafia intracorpórea videolaparoscópicas. Constatou-se ausência de rugosidades estomacais. Ao exame histológico, observou-se atrofia das células principais e parietais da mucosa gástrica. O quadro clínico permitiu o diagnóstico de gastrite crônica atrófica. O animal foi medicado com terapia imunossupressora e apresentou remissão completa dos sinais clínicos.This report describes a case of chronic atrophic gastritis. A two-year-old female mongrel dog showed chronic emesis. The complete blood count serum chemistry and urinalysis values were within the normal limits. Radiographs revealed no alterations. Abdominal evaluation, gastrotomy, gastroscopy, gastrectomy and intracorporeal stomach suture were done by laparoscopic approach. Absence of gastric villous was noticed through laparoscopic biopsy. The microscopic analysis reveled parietal and principal gastric mucosal cells atrophy, which, associated with clinical signs, allowed the chronic atrophic gastritis diagnosis. The animal was treated and clinical signs complete remission was observed.

  18. Gambaran Perubahan Sosial Berdasarkan Strategi Kreatif Iklan Televisi (Analisis Isi Gambaran Perubahan Sosial Berdasarkan Strategi Kreatif Iklan Televisi A Mild Tahun 2005-2014)

    OpenAIRE

    PATRICIA, EUNIKE AYU

    2014-01-01

    The Marketing Communications Planning Framework (MCPF) menunjukkan adanya hubungan saling pengaruh antar elemen context analysis dan promotional methods. Penelitian ini melihat bagaimana perubahan sosial sebagai konteks dalam komunikasi pemasaran digambarkan dalam strategi kreatif iklan televisi sebagai salah satu media dalam metode pemasaran. Peneliti menggunakan Adaptive Structuration Theory sebagai teori dasar dalam penelitian ini. Dimensi perubahan sosial menurut Himes dan Moore dijadikan...

  19. Distracción osteogénica alveolar: una alternativa en la reconstrucción de rebordes alveolares atróficos: Descripción de 10 casos Alveolar distraction osteogenesis: an alternative in the reconstruction of atrophic alveolar ridges: Report of 10 cases

    Directory of Open Access Journals (Sweden)

    P.E. Maurette O’Brien

    2004-02-01

    Full Text Available La distracción osteogénica alveolar (DOA es un método alternativo para la reconstrucción de rebordes alveolares atróficos que ofrece un resultado previsible y que disminuye los tiempo de espera entre la reconstrucción del reborde alveolar atrófico y la colocación de los implantes óseo-integrados, en comparación con los métodos tradicionalmente utilizados. Fueron atendidos 10 pacientes que presentaban deficiencia de reborde alveolar mandibular y/o maxilar por medio de distracción osteogénica, utilizando un dispositivo yuxtaoseo (Conexión Implant System® - SP-Brasil. Todos los pacientes fueron atendidos de forma ambulatoria, bajo anestesia local y sedación conciente, comenzando la activación del dispositivo a los 7 días posteriores a la instalación, con un patrón de activación de 1 mm diarios hasta alcanzar la altura ósea deseada. Posteriormente se aguardaron 10 semanas como parte del periodo de consolidación ósea y se realizo la colocación de los implantes oseointegrados y local y el retiro del dispositivo de distracción, pudiéndose comprobar clínica y radiográficamente la ganancia de la altura y volumen óseo necesario para la rehabilitación por medio de implantes.The alveolar distraction osteogenesis is an alternative method for the reconstruction of atrophic alveolar ridges with success, that decrease the time of wait between the reconstruction of the alveolar ridge and the placement of the osseointegrated implants in comparison with the traditionally used methods. 10 patients that presented deficiency of the alveolar ridge in the maxilla and/or mandible were assisted by means of distraction osteogenesis, using a juxtaosseous device (Conexion Implant System® - SP-Brazil. All the patients were assisted of form ambulatory, under local anesthesia and conscientious sedation, beginning the activation from the device 7 days later to the installation, with a pattern of activation 1 mm diary until reaching the wanted

  20. Analisis Komparatif Kinerja Keuangan antara Bank Syariah dan Bank Konvensional Berdasarkan Metode Camel

    Directory of Open Access Journals (Sweden)

    Armanto Witjaksono

    2011-05-01

    Full Text Available The main objective of this paper is to give an overview of comparative financial performance of Islamic banks with conventional banks based on the CAMEL method. The main question would be answered to know there is difference in the performance of conventional banking and Islamic banking are analyzed by using the ratio CAMEL. To prove the hypothesis that there is no significant difference between the bank's financial performance Islamic and conventional banks (Ho or there are significant differences between the financial performance of Islamic banks and conventional banks (Hi. Researchers used a parametric statistical technique, which consists of test data normality using the Kolmogorov Smirnov test and QQ plots, test of homogeneity using the F test (Levene's Test, and Independent Sample T-Test with significant value 5% confidence level (1 - α = 95%. The results showed that the ratio of data CAMEL Islamic banks and conventional banks in normal distribution and homogeneous. Then it can be concluded that the variable CAR, NPL, and LDR between conventional banks and Islamic banks have significant differences, while the ROA and ROE of the two types of banking industry is not significantly different or relatively the same.

  1. STUDI KUALITAS PERAIRAN TELUK EKAS BERDASARKAN KOMPONEN FISIKA-KIMIA PADA BULAN MARET 2014

    Directory of Open Access Journals (Sweden)

    Muhammad Ramdhan

    2015-11-01

    Full Text Available The research was conducted on 9 to 15 March 2014 in the Ekas Bay region, East Lombok, West Nusa Tenggara Province. Water quality data acquisitions was done with purposive sampling by using a multiparameter WQC-24 TOA-DKK, floating drought in situ current measurement and water sampling for laboratory check. The parameters measured are temperature, salinity, DO, dT, pH, flow speed, Turbidity, TOM, NO2, NH3, PO4P, Chlorophyll and TSS. Data analysis using descriptive and Biplot graph analysis. The results showed range of values ​​for all parameters was almost in accordance with KepmennegLH No. 51 of 2004, based on physico-chemical parameters measured, still in good condition. Only the phosphate values ​​which average value of 0.41 mg / L is above the standard of Kepmenneg LH No. 51 of 2004 which is 0.015 mg / L. Permalink/DOI: http://dx.doi.org/10.15408/sd.v2i1.1378

  2. EVALUASI CEPAT DESAIN ELEMEN BALOK BETON BERTULANGAN TUNGGAL BERDASARKAN RASIO TULANGAN BALANCED

    Directory of Open Access Journals (Sweden)

    Agustinus Agus Setiawan

    2010-05-01

    Full Text Available Design procedure for reinforced concrete structure in Indonesia is provided by Tata Cara Perhitungan Struktur Beton, SNI 03-2847-2002. This code covers analysis method and basic assumptions that can be taken by a structural designer to design a reinforced concrete structure. An engineer has to design a flexural component, one of the structural component. One of the flexural component is beam element. SNI 03-2847-2002 adopt capacity design method in beam element design. This paper will be discuss a simple design procedure in beam element, based on ξ variable, which is ratio between ρ/ρb, with ρb is the balanced reinforcement ratio. The ξ value is limited to 0,75 maximum in SNI 03-2847-2002. A detail failure is guarantee in a beam element with ξ under 0,75. The analysis that have been done before, produce the correlation between Mn/bd2 and ξ, which can be a tool for design a beam element. A simple calculation use the table (Mn/bd2 vs ξ will be present at the end of this paper.

  3. Identifikasi Kelelawar Pemakan Buah Asal Sulawesi Berdasarkan Morfometri (THE MORPHOMETRIC IDENTIFICATION OF CELEBES FRUIT BATS

    Directory of Open Access Journals (Sweden)

    Tiltje Andretha Ransaleleh

    2013-12-01

    Full Text Available The bat is very important for human life, because of their  role as pollinator of plants, as a producer oforganic fertilizer,  and as food.  In Northern Celebes fruit eating bats serve as exotic food, so the presenceof bats were threatened to be extinct due to uncontrolled hunting.  The changes of the forest for plantationlands, damage the habitats and the bats were forced to migrate out.   The aim of the study was to identifythe fruit eating bats of Celebes.  Morphometry of body size, skull,  and physical characteristics were usedin determining the types and distribution  of fruit eating bats in Celebes. The field survey was carried outat the hunting area, bat dealers, and bat sellers. The collected data were analyzed by using descriptivemethod and interpreted by narrating to describe the entire study. The result show that  there were fivetypes of fruit eating bats : i.e.  kalong sulawesi (Acerodon celebensis,  paniki pallas (Nyctimene cephalotes,codot wallet (Thoopterus nigrescens, nyap biasa (Rousettus amplexicaudatus, and  kalong hitam  (Pteropusalecto.

  4. Hubungan FIB-4 Index Dengan Derajat Fibrosis Hati Pada Hepatitis B Kronik Berdasarkan Fibroscan

    OpenAIRE

    Siregar, Yuswita Santi

    2016-01-01

    Introduction : The accurate evaluation of liver fibrosis in chronic liver disease is mandatory to appraise therapeutic indications and prognosis, because complications mainly occur in patients in advanced stages of their disease. Noninvasive test to asses hepatic fibrosis have been developed, wich combine several biochemical parameters. Transient elastography (FibroSscan) a morphological method that measures liver stiffnes has been evaluated. Aim : To evaluate the correlation of FIB-...

  5. Evaluasi Shift Kerja dan Penentuan Waktu Standar PT X Berdasarkan Beban Kerja

    Directory of Open Access Journals (Sweden)

    Trisna Mesra

    2016-04-01

    Full Text Available PT. X is one of the companies located in Dumai Industrial Region, produces fertilizer. This company has three production plants. Production capacity of the first plant is 450 tons/day, the second plant produces 750 tons / day and the third plant produces 700 tons / day. PT. X is supported by 2 sub companies, they are PT. A and PT. B. PT. A operates 5 bagging lines and PT. B operates 3 bagging lines. The fertilizer bagging process has three steps where each step has its own work station: putting the labelled sack to the hook followed by fiiling the sack with fertilizer, installing the tie cable, and sewing the fertilizer filled sack. From the initial survey, it was found that the processingtime is not optimal to reach production target. PT. A and B have to set 24 working hours per day to run the target resulting on frequent overtime, excessive fatique and negative impact of physically and mentally for the workers. Author’s hypothesis is the current condition has been one of the reasons for a decline of the production output. Therefore, the purposes of the research are to find the impact of current working hours and shift work pattern on physical and psycological respon of the workers and to calculate standard time required to complete one cycle of bagging process. This standard time is very crucial to determine production target thus determine the optimal working hours and shift rotation. Measuring of heart rate arecollecting results of questionnaire of NASA-TLX are the parameters used to evaluate physical and psychological respon of workers. Two workers from different group worker, different shift work, from PT. A and PT. B were selected as samples to evaluate their heart rate pattern. The questioner spread out to all workers. Time standard was determined by observing and taking data of 40 bagging cycles for each shift work. The results show that shift work affecting the physiological response of workers in PT. B in the category between moderate to extreme especially for working element of arranging fertilizer sack to the pallet. This result was found for the two group workers. Frequent short rests between times are required to provide quick recovery for the workers and reduce excessive fatique. Average standard time complete one bagging cycle was 0.12 minutes and 0.13 minutes for PT. A and PT. B, respectively. These standard times can be used to set the production output target for the two companies. Last, short rotation shift work with additional number of workers is recommended for the shift pattern.

  6. Penskalaan Butir Format Respons Pilihan dan Respons Bebas Berdasarkan Model Rasch dan Partial Credit

    Directory of Open Access Journals (Sweden)

    Eko Hariadi

    2007-06-01

    Full Text Available Penelitian melihat pengaruh jumlah parameter butir, kategori respons bebas (RB, pengaruh sampel terhadap akurasi estimasi parameter kemampuan untuk menghasilkan estimasi yang stabil dan pengaruh pembobotan butir RP dan butir RB terhadap kesalahan baku. Penelitian dalam dua tahap, simulasi menggunakan 30 kondisi dengan replikasi 50 dengan variabel panjang tes, jumlah kategori, dan jumlah parameter butir, dan analisis deskriptif, dilanjutkan penerapan penskalaan gabungan butir tipe respons pilihan (rp dan butir respons bebas (rb pada konstruksi tes elektronika yang terdiri 40 butir pilihan ganda dan 4 butir jawaban tersusun, 3 butir memiliki lima kategori jawaban dan 1 butir dengan 4 kategori jawaban, melibatkan 355 siswa. Hasil penelitian menunjukkan: ukuran sampel kurang berpengaruh pada root mean square error atau (RSME> dan korelasi antara 9 dengan 0, namun berpengaruh terhadap akurasi estimasi parameter butir pilihan ganda (/>/y,dan parameter butir respons tersusun (3^- Jumlah parameter butir berpengaruh terhadap parameter kemampuan, tetapi tidak berpengaruh terhadap akurasi dari b^, dan S„,. Estimasi dari parameter tingkat kesulitan butir jawaban tersusun tiga kategori lebih akurat daripada butir jawaban tersusun lima kategori. Estimasi tahan {robust untuk parameter kesulitan butir jawaban tersusun 5 kategori memerlukan sampel minimal 250 responden, sedangkan untuk butir respons tersusun 3 kategori memerlukan sampel minimal 100 responden. Estimasi parameter kemampuan dari skor total (0^^ tidak sama dengan rata-rata jumlah tbeta dari masing-masing subtes (0^ + 0CR. Theta dari tes yang dikalibrasi bersama-sama berbeda dengan theta dari total subtes yang dikalibrasi secara terpisah. Korelasi kemampuan yang mengunakan pembobotan dan kemampuan tanpa pembobotan mempunyai suatu rentang dari 0,988 sampai 0,948. Kata kunci: penyekaiaan, model rash dan partial credit.

  7. Kemampuan Ekstrak Daun Zodia (Evodia suaveolens) Sebagai Repellent Nyamuk Aedes aegypti Berdasarkan Lama Penggunaannya

    OpenAIRE

    Sianipar, Melati Agnes Anggreini

    2011-01-01

    Dengue Hemorrhagic Fever (DHF) disease is a contagious disease that caused by dengue virus and infected by Ae. aegypti. Since reported in 1968, this disease has become endemic and reported every year in Indonesia. One of the way to avoid it by using anti mosquitoes lotion which generally made by synthetic chemistry. Therefore, it necessary to find natural substance to avoid mosquitoes, one of the natural substances that can use is from zodia leaves (Evodia suaveolens) as repellent. The a...

  8. Manfaat Informasi Akuntansi Untuk Memprediksi Risiko Investasi Saham Berdasarkan Pendekatan Decision Usefulness

    OpenAIRE

    Zarah Puspitaningtyas

    2010-01-01

    This study is to explain the usefulness of accounting information to predict the risk of stock investment based on the decision usefulness approach, technically shown through the effect of variable accounting on risk of stock investment. Based on analysis of 13 companies over a period of five years (2001-2005) by using multiple linear regression, the model shows that the current ratio significantly influence the risk of stock investment. However, signs of regression coefficients are not consi...

  9. ANALISIS TINGKAT KAPABILITAS SISTEM INFORMASI RUMAH SAKIT BERDASARKAN COBIT 5 (MEA01) PADA RSUD TUGUREJO SEMARANG

    OpenAIRE

    Ariel Bagus Nugroho; Amiq Fahmi

    2015-01-01

    RSUD Tugurejo Semarang merupakan Rumah Sakit kelas B milik pemerintah Provinsi Jawa Tengah yang menyediakan pelayanan jasa untuk masyarakat. Dalam manajemen pengolahan data, RSUD Tugurejo Semarang telah menggunakan sistem informasi rumah sakit. Untuk mengetahui apakah sistem informasi telah berjalan seperti yang diharapkan, maka perlu dilakukan analisis tatakelola teknologi informasi. Pada penelitian ini analisis tatakelola teknologi informasi berfokus pada proses pengawasan, evaluasi dan pen...

  10. POLA KONSUMSI DAN PERMINTAAN PANGAN POKOK BERDASARKAN ANALISIS DATA SUSENAS 2005

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    Anna Vipta Resti Mauludyani

    2012-03-01

    Full Text Available 800x600 Normal 0 false false false IN X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Calibri","sans-serif"; mso-bidi-font-family:"Times New Roman";} Food demand can be found dynamic, altering by the change in price and income. How great the influence of those changes to food consumption is the important information as the basic consideration for government in creating policy related to food consumption. The objective of the research, which has survey design, was to analyze staple food consumption and demand pattern in household in Indonesia. The research was conducted in Bogor, from March to June 2008 by using secondary data Susenas year 2005 with 64.709 households as the samples. The staple food analyzed were rice, corn, cassava, sweet potato, wheat flour and its derived product (instant noodle, noodle. Data was processed by SAS program version 6.12 and Double-Log Regression econometrical model. Then, it was analyzed descriptively. Result shows that rice has the biggest expenditure proportion among staple food. Almost all staple food is obtained by purchasing, except in corn, cassava, and sweet potatoes. Energy contribution from rice reaches half of total energy consumption. The consumption of rice, corn, cassava, sweet potatoes, wheat flour and its derived product, instant noodle, and wheat noodle per capita per year are 100.52 kg, 3.36 kg, 11.67 kg, 4.10 kg, 5.09 kg, 3.39 kg, and 0.22 kg. Staple food which has consumption participation level almost 100% is rice. The own price elasticity of staple foods are not elastic, except in corn, wheat flour and its derived product. The income elasticity of all staple foods is not elastic in all category of region and income stratification. The demand elasticity of staple food give some implications on consumption and food consumption improvement, they are: 1 raising price of staple food can decrease consumption, thus, price stability is very important, 2 almost all staple foods has not elastic income elasticity, so that food consumption improvement needs great stimulus of increasing income, 3 poor household is greatly influenced by the increasing of price, thus, food consumption improvement for them must be conducted by doing many relevant efforts, 4 target of decreasing rice consumption is still cannot be reached, so that staple food diversification must be continuously developed, 5 in order to develop local food consumption, like corn, cassava, and sweet potato, the development of agro industry based on local food and Communication Information Education (CIE about food consumption diversification are essential to be conducted. Keywords: consumption and demand pattern, staple food, Susenas

  11. Manfaat Informasi Akuntansi Untuk Memprediksi Risiko Investasi Saham Berdasarkan Pendekatan Decision Usefulness

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    Zarah Puspitaningtyas

    2010-12-01

    Full Text Available This study is to explain the usefulness of accounting information to predict the risk of stock investment based on the decision usefulness approach, technically shown through the effect of variable accounting on risk of stock investment. Based on analysis of 13 companies over a period of five years (2001-2005 by using multiple linear regression, the model shows that the current ratio significantly influence the risk of stock investment. However, signs of regression coefficients are not consistent with the predictions of the study. The results indicate that the current ratio has positive influence on the risk of stock investment. F test shows that the regression model with the current ratio as independent variables significantly influence the risk of stock investment. Meanwhile, the regression model with independent variables of current ratio, debt equity ratio, total assets turnover, return on investment, sales growth, and price earnings ratio do not significantly influence the risk of stock investment. The findings suggest that accounting information has relatively little ability to explain variations in risk of stock investment. The validation of regression models shows no difference between the real investment risk and investment risk prediction, meaning that accounting information is useful for predicting the risk of stock investment.

  12. POLA PENYAKIT SEBAB KEMATIAN DI JAWA - BALI BERDASARKAN SURVEI KESEHATAN RUMAH TANGGA 1995

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    Sarimawar Djaja

    2012-09-01

    Full Text Available In Indonesia, the National Household Health Survey (SKRT is still the main source of information on causes of death, which is now integrated with the National Socio Economic Survey (Susenas. The SKRT uses technique of verbal autopsy with open questions then classifies the diseases using ICD 10. There were 1719 cases obtained with 97,8% response rate of deaths reported by Susenas to the SKRT. Eventhough there are some limitations, the 1995 SKRT using proportion analysis is able to show the pattern of cause of death. The pattern in Java-Bali shows the current trend of epidemiologic transition - the changing pattern of infectious diseases towards degenerative diseases. For the first time the 1995 SKRT reveals the replacement of dominant infectious diseases by circulatory (cardio as well as cerebrovascular diseases. The SKRT observation in the period of 1980 -1995 shows that the transition has happened with rapid trend compared to the England and Wales experience. Yet infectious diseases are still the important cause of death particularly among young age groups and population in the rural areas. Circulatory diseases do not affect only the old age groups but the productive age groups as well. The circulatory diseases can not be disregarded in the rural areas. This condition affects both sexes male and female. Maternal together with perinatal diseases are still quite substantial causes of death.

  13. Pengembangan Tungku Gasifikasi Arang Biomassa Tipe Natural Draft Gasification Berdasarkan Analisis Computational Fluid Dynamics (CFD

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    Erlanda Augupta Pane

    2014-10-01

    Full Text Available A biomass stove based on natural draft gasification (NDG has been developed in a previous study (Nelwa, et al. 2013 by using simulation based on heat transfer and equilibrium modeling. In this study, a CFD simulation was performed in order to analyze the effect of chimney height, and inlet hole diameter of the stove to the performance of the stove. The results of simulation showed that power produced by stove was between 1863.9 J/s until 2585.7 J/s, and its gasification efficiency was 67.11%. The results of simulation also showed that charcoal gasification produces combustible gases (CO, CH4, and H2 at the bottom and the center of stove, and then they were oxidized by secondary air at the top of stove. This oxidation reaction produces sufficient heat energy which can be used for cooking process.

  14. MODEL PREDIKSI KEJADIAN DEMAM BERDARAH DENGUE (DBD BERDASARKAN FAKTOR IKLIM DI KOTA BOGOR, JAWA BARAT

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    Jusniar Ariati

    2015-01-01

    Full Text Available AbstractDengue Hemorrhagic Fever (DHF presents a serious health problem in Indonesia. Dengue viruses are transmitted to human through the biting of infected mosquitoes, especially Aedes aegypti and Ae. albopictus.The occurrence of variation and climate change will Affect the growth areas of mosquitoes. This situation can influence on the emergence of dengue fever cases. In this paper will discuss the predictions of the mathematical model of considering the incidence of DHF with climatic factors. The research design was a retrospective study with the data collected is dengue incidence and climate include temperature, rainfall, humidity and rainy days since 2002-2010. Data analysis was performed using Minitab 16.0 software statistical time series. The results showed that R2 varied between 0.65 to 0.99. The highest R2 value of the regression equation obtained in August, September and October is 0.99 and the lowest in April with a R2 value of 0.65. The results of predictions based on 4 predictors (precipitation, rainy days, temperature and humidity with the incidence of DHF is actually not much different, except in April. It can be concluded that according to linear predictive models of dengue is influenced by climatic factors (precipitation, rainy days, temperature and humidity 2 months before and 1 month prior dengue incidence.Keywords : Dhf, Climate, Prediction ModelAbstrakDemam Berdarah Dengue (DBD merupakan salah satu masalah kesehatan di Indonesia. Aedes aegyptisebagai vektor utama penyakit DD/DBD kehidupannya dipengaruhi oleh faktor iklim, diantaranya suhu, kelembaban udara, curah hujan dan hari hujan. Berbagai upaya pengendalian  telah   dilakukan  namun  belum   menurunkan  jumlah  kasus  secara  signifikan, sehingga diperlukan model untuk memprediksi kejadian DBD di suatu wilayah sehingga kejadiannya dapat diantisipasi. Dalam tulisan ini akan membahas model matematika prediksi kejadian DBD dengan mempertimbangkan faktor iklim. Disain penelitian adalah studi retrospektif dengan data yang dikumpulkan adalah kejadian DBD dan iklim yang meliputi suhu, curah hujan, hari hujan dan kelembaban sejak tahun 2002-2010. Analisis data dilakukan secara time series menggunakan minitab statistical software 16.0. Hasil analisis mendapatkan model prediksi kejadian DBD dipengaruhi oleh fungsi empat faktor iklim, yaitu curah hujan, hari hujan, suhu dan kelembaban pada lag time2.Kata kunci : Demam Berdarah, Faktor Iklim, Model Prediksi

  15. Revenue Discretionary Model Pengukuran Manajemen Laba: Berdasarkan Sektor Industri Manufaktur di Bursa Efek Indonesia

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    Nieken Herma Sari

    2014-01-01

    Full Text Available Earnings management is a management action to manage the profitability of a company. The purpose of this study is to indicate the accrual earning management using revenue discretionary model. This study adopts the research conducted by Stubben (2010 where there are two measurement models to detect the presence of accrual earnings management. The measurement model is the discretionary revenue model consists of conditional revenue model and revenue model. Object of this research is the companies listed in Indonesia Stock Exchange. The technique of data analysis is descriptive statistics to determine the residual value in each industry sector. These results prove that the revenue model is able to indicate the industrial sector 8 of the total 13 industries in the manufacturing companies indicated accrual earnings management. Conditional revenue model is able to indicate the industrial sector 11 of the total 18 sectors industry that overall indicated accrual earnings management.

  16. PENGEMBANGAN MODEL EVALUASI PROSES PEMBELAJARAN MATEMATIKA DI SMP BERDASARKAN KURIKULUM 2013

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    Sugiyanto Sugiyanto

    2015-06-01

    Abstract This studi aims to develop a model for an evaluation of mathematics learning processes at junior high schools (JHSs based on Curriculum 2013 and evaluate the effectiveness of the model develo-ped. This was a research and development study. The subject of the research was the teachers, principals, and supervisors. The data collecting instruments were questionnaires and observational sheet. The development process was carried out in 4 stages namely: (1 preliminary investigation; (2 planning development, validation; (3 tryout; (4 product finalization. The try out was perform-ed in 3 stages with the increasing number of participants for each stage. The learning process evaluation model was supported by instrument to assess lesson plans, learning implementation, and the implementation of learning outcome assessment. The content validity was assessed through focus group discussion (FGD. The construct validity was assessed by the factor analysis, and the reliabilities were estimated by the Intraclass Correlation Coefficient (ICC. The result of the research was a model of evaluation of mathematics learning processes at junior high schools (JHSs based on Curriculum 2013, consisting of procedures, evaluation guideline and the well de-signed instruments. Based on the try out, all of the instruments have had good suitability, because construct validity and reliability meet all of the academic requirements. According to the evalua-tion from the practitioners and the model users, the model of the evaluation of mathematics learn-ing processes at junior high schools (JHSs based on Curriculum 2013 was very effective to be implemented. Keywords: development model, evaluation of learning processes, SMP mathematics

  17. STRUKTUR KONSEP MAHASISWA CALON GURU TENTANG MEDAN ELEKTROSTATIK BERDASARKAN ANALISIS FRAMING

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    S. E. Nugroho

    2012-01-01

    Full Text Available Penelitian ini bertujuan untuk mengeksplorasi struktur konsep mahasiswa calon guru tentang medan elektrostatik. Data diperolehdengan menggunakan metode thinks-aloud, didukung metode lain yaitu wawancara, diskusi, dan tes tertulis Responden penelitianterdiri atas delapan mahasiswa semester 2 yang telah menempuh fisika dasar sebagai kelompok I, dan sembilan mahasiswasemester 6 yang telah menempuh mata kuliah kelistrikan dan kemagnetan sebagai kelompok II. Mahasiswa tersebut diberipertanyaan real-worlds yang menyangkut konsep dasar medan dan memerlukan jawaban secara spontan. Hasil studi inimenunjukan bahwa (1 struktur konsep mahasiswa mengenai medan elektrostatik pada umumnya mengalami fragmentasi; (2mahasiswa mengalami kerancuan dalam memahami konsep medan elektrostatik; (3 Pada umumnya, mahasiswa masihberlandaskan konsep gaya dalam memahami tentang medan. The purpose of this research is to explore the concept structure of electrostatics field of teacher candidate student. In thisexperiment, we used thinks-aloud method and varied by others (interview, discussion and written-test. Respondent consisted ofeight students of second semester passing Basic Physics subject as the first group and nine students of sixth semester passingelectricity and magnetism subject as the second group. On these students the real world questions related to concept of field andneeded spontaneous answer are asked. The study result shows that (1 in general the concept structure of electrostatics field ofstudents undergoes fragmenting process; (2 the students experience contamination in understanding the electrostatics fieldconcept, and (3 in general the students are still based on the force concept in understanding the field.Keywords: framing analysis, teacher candidate student, concept structure

  18. Pemberian Pupuk N, P, dan K Berdasarkan Pengelolaan Hara Spesifik Lokasi untuk Meningkatkan Produktivitas Kedelai (Review

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    KARSIDI PERMADI

    2016-01-01

    Full Text Available Applicationof N, P, and K Fertilizer Based on Site-Specific Nutrient Management for An increase of Productivity of Soybean. Soybean productivity in Indonesia is still low, it is about 1.25 t ha-1.This is due to the same dose of fertilizer in all location.In this case, fertilization is on the recommendation i.e. 25-75 kg urea ha-1 + 50-100 kg SP-36 ha-1 + 50-100 kg KCl ha-1 for soybean in all locations.Though each location farmland have different levels of soil fertility. Soybean commodities can compete with other plants when minimum productivity of 2.18 t ha-1 of dry beans, it is able to provide a higher profit than corn. To that end, soybean fertilizer recommended by site-specific nutrient management (SSNM by using the method of paddy soil test device, and dry soil test device that can determine soil nutrient classes to categories of low, medium and high.N fertilizer on soil classes, including low, medium, and high dose respectively at 174, 152, and 117 kg urea ha-1. For P fertilizer application on soil nutrient classes, including low, medium, and high doserespectively at 104,80, and 40 kg ha-1 SP-36. Likewise K fertilizer application on soil nutrient classes, including low, medium, and high each at doses of 210, 190, and 150 kg ha-1 KCl. In addition, supported by the use of new varieties of soybean large seed that high productivity and achieve the results expected in all locations soybean.Then the government provides support for input subsidies in the form of primary production facilities such as seed, fertilizer, and pesticides that farmers are interested in returning to planting soybeans and soybean prices ensure incentives for farmers. Things like this will spur farmers to increase productivity so that soybean self-sufficiency is achieved. In the end domestic soybean demand can be met so that the government no longer to import soybeans.

  19. EVALUASI SISTEM INFORMASI KENDARAAN (GFSERVICE- BINUS UNIVERSITY BERDASARKAN KERANGKA COBIT PADA DOMAIN DELIVERY AND SUPPORT

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    Tangkas Udiono

    2015-06-01

    Full Text Available Information Systems Audit is an activity that is carried out in a company that aims to ensure compliance with existing policies. General Services is one of the units in BINUS University whose activities in the form of services such as Transportation and Document Courier, Copy Centre and General Facility. However, this study is limited to the Transportation and Document Courier unit that uses an online reservation application gfservice.binus.ac.id. Maturity level of the existing information systems in Directorate General Services specifically Transportation and Document Courier based on COBIT Maturity Model is 2,951 (Repeatable but Intuitive or approaching from the standards set by the International is 3:00, which means the process has been documented and communicated well. This study also conducted an evaluation of the quality of information systems and quality of information available. The result is positive impact on users' satisfaction, that means the increases of information quality and the quality of information systems of GF Services would increase student and employee satisfaction, but it also explained the factors that affect success utilization of existing information systems in order to rise to the level to 4, which means managed and Measurable. The results of the regression analysis and correlation between the quality factors of information systems and the quality of information on student satisfaction and employee are 38.2% and 48.1%; while the rest is influenced by other factors not addressed in this study.

  20. Perancangan Fasilitas Berdasarkan Prinsip-Prinsip Ergonomi Pada Bagian Sortasi Udang DI PT. Central Windu Sejati

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    Hendrik P. Simbolon

    2010-01-01

    PT. Central Windu Sejati adalah salah satu perusahaan yang bergerak di dalam bidang pembekuan udang. Produk-produk yang dihasilkan kebanyakan diekspor ke luar negeri. Kualitas dan ketepatan pengiriman adalah salah satu persyaratan yang harus dicapai agar kepercayaan akan produk yang dihasilkan dapat diterima oleh konsumen di luar negeri. Produktivitas adalah salah satu yang sangat diperhatikan oleh perusahaan dalam kegiatan produksi agar target produksi dapat dicapai dengan tepat. Salah s...

  1. Modifikasi Mesin Pemanen Udang/Ikan Tipe Vakum berdasarkan Analisis Antropometri

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    Diza Puspa Arista

    2013-04-01

    Full Text Available Many considerations was used when designing a tool or machine, one of that is ergonomics. Ergonomics makes the designing was effective and efficient in terms of the system, and operator’s comfort and safety also. The objective of this study is to determine the condition of operator in carrying out the work system of machine with simulation and ergonomics analysis using CATIA P3 V5R20, then modify it according to ergonomics principles that related to the posture of Indonesian people average (anthropometry, to get a comfortable working position, safe, and productive. Anthropometric data was used are male farmer’s data in Dramaga, Bogor. The object that would analyzed is the vacuum type shrimp or fish harvester. The results are a color sign and the final score that compared with RULA Method. From the simulation can be seen that the best Final Score is when operated the intake hole cap with the value of 2, the color code is green and acceptable. The worst result is when cranked the engine with the value of 4 and investige further. Modification was made to displace the engine position and addition the components for footing. The results of ergonomics simulation after modified was better than before.

  2. BESARAN MASALAH GIZI BALITA KABUPATEN BOGOR BERDASARKAN BAKU ANTROPOMETRI NCHS DAN WHO 2005

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    Iman Sumarno

    2012-11-01

    Full Text Available The Magnitude of Malnutrition Among Under-Five In The District Of Bogor Using Anthropometric Standard Of NCHS And WHO 2005.Background: In the early June 2006 WHO introduced new Anthropometric standard. The WHO recommended the Southeast Asian countries to adopt the new standard. The standard is supported by software for data analysis. There seem no objections in adopting the standard. However, the experts group of Indonesian Nutrition Association demands to evaluate the standard, before Indonesia use it in the nutrition program. This paper is one of the activities in evaluating the impact of adopting new standard to the magnitude of malnutrition.Methods: This exercise use the data of Growth Monitoring Survey in the District of Bogor. The data were proccessed using the WHO anthropometric software. The data were analysed descriptively.Result: It was found that there is no significant difference in the prevalence of wasting, between using the NCHS and WHO standard (10.7% vs 11.1%. But the prevalence of wasting is doubling in the age group 0-5 months (7.8% vs 15.7%. The prevalence of stunted slightly increase, (14.7% by NCHS versus 18% by WHO. The prevalence of stunting is 34.1% by NCHS and 39.2% by WHO. The prevalence of severe underweight is the same; however, the prevalence of underweight is on the other way around. The prevalence is 20.2% by NCHS and become 15.9% by the WHO it is interesting to note when involving weight the prevalence always higher by WHO in the age group of 0-5 months.Conclusion: The lower prevalence of underweight may cause a problem in the program because this is the parameter that is used in the program. The target of nutrition program is set 20% underweight if we use WHO the prevalence now from 20.2% became 15%.Keywords: nutritional status index, anthropometric standard

  3. Modifikasi Mesin Pemanen Udang/Ikan Tipe Vakum berdasarkan Analisis Antropometri

    OpenAIRE

    Diza Puspa Arista; Sam Herodian; Muhamad Yulianto

    2013-01-01

    Many considerations was used when designing a tool or machine, one of that is ergonomics. Ergonomics makes the designing was effective and efficient in terms of the system, and operator’s comfort and safety also. The objective of this study is to determine the condition of operator in carrying out the work system of machine with simulation and ergonomics analysis using CATIA P3 V5R20, then modify it according to ergonomics principles that related to the posture of Indonesian people average (a...

  4. HUBUNGAN GANGGUAN GIZI ANAK BALITA BERDASARKAN INDEKS ANTROPOMETRI TUNGGAL DAN KOMBINASI DENGAN MORBIDITAS DAN IMPLIKASINYA

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    Yekti Widodo

    2012-11-01

    Full Text Available Background: Under weight, stunting and wasting are single anthropometry index that are not enough to predict prevalence of under nutrition. Using underweight as indicator to calculate prevalence of under nutrition can be under estimate or over estimate because of underweight is the result of stunting and wasting, not because of the sum of stunting and wasting. Objectives: The aim of this data analysis was to compare the relationship of prevalence of children under five under nutrition with morbidity between single and composite indices. Methods: The source of data from Health Research Basic (Riset Kesehatan Dasar 2007/2008. Under five year nutritional status was analysis with WHO Anthro 2009 software. The statistically analysis conducted with X2 statistical test. Results: The prevalence of severe malnutrition (z-score <-3 SD, WH0-2005 based on single anthropometry index for underweight (weight/age was 4.8%, stunting (height/age was 18.8%, and wasting (weight/height was 6.2%, however based on combination indices, the prevalence of severe malnutrition was 25.5%. The severe and moderate malnutrition (z-score <-2 SD, WH0-2005 based on single anthropometry indices was 19.0%, stunting was 37.0%, and wasting was 14.4%, whereas based on combination indeces the prevalence of severe malnutrition and mild malnutrition was 50.1%. The risk of upper respiratory infection, diarrhea, and measles was higher (odd ratio: 1.1- 1.4 on children with combination indices than single anthropometry indices. Conclusions: Composite anthropometry analyses could explain under five children that severely and totally malnourished. Based on combination indices one out of four under five children was severely malnourished and one out of two children was malnutrition. The morbidity was higher on children with composite indices than single anthropometry indices.   Keywords: composite indices, anthropometry, severe-malnourished prevalence

  5. Analisis Kondisi Kerja Operator Pembuatan Kuali Untuk Merancang Fasilitas Kerja Berdasarkan Pendekatan Ergonomi

    OpenAIRE

    Benny, Sepri

    2014-01-01

    Penelitian ini dilakukan di UD Satria yang beralamatkan di Brigjen Katamso Gg Satria No. 20, Medan UD Satria merupakan industri kecil yang bernaung dalam usaha pandai besi dengan menghasilkan produk kuali. Penelitian ini berfokus pada pembuatan kuali yaitu di stasiun pemukulan halus kuali. Pada proses pemukulan halus masih dilakukan secara manual oleh seorang operator. Operator sering mengalami keluhan sakit pada beberapa bagian tubuh. Hal ini disebabkan karena fasilitas kerja tidak ergonomis...

  6. NOVEL GADIS PANTAI KARYA PRAMOEDYA ANANTA TOER: ANALISIS BERDASARKAN KONSEP ANDROGINI

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    Supriyadi

    2005-06-01

    Full Text Available Feminism firstly developed in France, but recently it has spread all over the world covering all aspects of life. particularly in the relations between men and women. Women feel that they have not had the same rights as men. A branch of feminism which attempts to abridge the relations is androgyny, with a concept of harmony where men and women should work together to reach social welfare. The concept is interesting particularly when applied in literary works written by male writers. Feminism then is also seen on men perspective. Gadis Pantai is one of Indonesian novels written by Pramoedya Anantya Toer which discusses feminism. The story is about a girl, whose name is Gadis Pantai, living in Rembang in the beginning of the twentieth century. She is the symbol of ordinary or laymen. She suffered when Bendoro, the symbol of noblemen, took her as his wife. The story is about the struggle between the two symbols.

  7. KEDUDUKAN KETETAPAN MPR BERDASARKAN UU NO. 12 TAHUN 2011 TENTANG PEMBENTUKAN PERATURAN PERUNDANG-UNDANGAN

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    Delfina Gusman

    2012-03-01

    Full Text Available MPR Decree in resettlement of legislation under the Constitution of 1945 on the basis of Law No. 12 Year 2011 cocerning formation of legislation gives rise to a variety of polemics in the position of the MPR as State institutions. Provisions of the MPR is considered to have urgency in Indonesia's system, because the MPR as State institutions is considered to still have the duty and the authority of its strategic, e.g: inauguration of President and Vice President, the establishment of the Constitution. MPR also made provisions as one of basic national legislation program.

  8. ANALISIS TINGKAT KAPABILITAS SISTEM INFORMASI RUMAH SAKIT BERDASARKAN COBIT 5 (MEA01 PADA RSUD TUGUREJO SEMARANG

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    Ariel Bagus Nugroho

    2015-11-01

    Full Text Available RSUD Tugurejo Semarang merupakan Rumah Sakit kelas B milik pemerintah Provinsi Jawa Tengah yang menyediakan pelayanan jasa untuk masyarakat. Dalam manajemen pengolahan data, RSUD Tugurejo Semarang telah menggunakan sistem informasi rumah sakit. Untuk mengetahui apakah sistem informasi telah berjalan seperti yang diharapkan, maka perlu dilakukan analisis tatakelola teknologi informasi. Pada penelitian ini analisis tatakelola teknologi informasi berfokus pada proses pengawasan, evaluasi dan penilaian kinerja, dan kesesuaian kinerja teknologi informasi, yaitu domain MEA01 pada framework COBIT 5.Tujuan penelitian ini untuk mengetahui tingkat kapabilitas dan strategi perbaikan untuk proses pengawasan, evaluasi dan penilaian kinerja dan kesesuaian TI. Metode pengumpulan  data penelitian ini dengan menggunakan studi dokumen, kuesioner dan wawancara kemudian dianalisis tingkat kapabilitas dan kesenjangan. Tingkat kapabilitas yang didapat dari hasil penelitian yaitu berada level 2 (managed. Untuk menaikkan tingkat kapabilitas pada level 3 maka maka perlu dilakukan secara bertahap strategi perbaikan pada PA 2.1, PA2.2, PA 3.1, dan 3.2. Kata kunci: Analisis Tata Kelola TI, COBIT 5, MEA01, Tingkat Kapabilitas, Rumah Sakit.

  9. ANALISIS KUALITAS PELAYANAN PADA BAGIAN TATA USAHA BERDASARKAN TINGKAT KEPUASAN MAHASISWA

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    Muzakiyah Muzakiyah

    2011-12-01

    Full Text Available Kepuasan pelanggan merupakan hasil (outcome yang dirasakan atas penggunanya produk atau jasa, sama atau melebihi harapan yang diinginkan. Pelayanan di bagian Tata Usaha idealnya lebih bersahabat, cepat dan akurat. Ini berarti orientasi layanan di bagian Tata Usaha harus didasarkan pada kebutuhan mahasiswa. Dalam penelitian ini metode yang digunakan dengan menggunakan Pendekatan Lean ServPerf (Lean Service dan Service Performance. Service Performance digunakan untuk menganalisis tingkat instrumen kinerja pelayanan bagian Tata Usaha. Lean Service digunakan untuk menghilangkan waste aktivitas yang tidak bernilai tambah. Hasil dari penelitian ini, terdapat 14 atribut pelayanan yang perlu dilakukan perbaikan untuk meningkatkan kualitas pelayanan di bagian Tata Usaha Fakultas.

  10. EVALUASI EMERGENCY RESPONS PLAN PADA KAPAL 42M CREW BOAT BERDASARKAN IMO INTERIM GUIDILINES

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    Mochamad Luqman Ashari

    2013-10-01

    Full Text Available Emergency situations that have tendency or potential harm can be happened any time and any where and carry some big damaging impacts to property damages, mental disturbances, injured and died victims. In shipping, emergencies can be happened in both at anchor or while doing loading and unloading activities at the port. The type of equipments used in emergency situations set out in SOLAS Chapter III (Life Saving Appliance and the guidelines for evacuation analysis on passenger ships refer to the IMO Guidelines Interm MSC/Circ.909. This study aimed to determine the muster station and evacuation time on the ship 42 M crewboats with a capacity of 101passengers. This ship consists of three parts of decks, these are lower part, main deck and bridge deck. The results showed that the placement of muster station is on the bridge deck with required wide 35.35 m2. Evacuation time need by the all passengers to reach the muster station on the bridge deck is 51.59 minutes, and this is in accordance with standard evacuation time (not more than 60 minutes.

  11. Identifikasi Kelelawar Pemakan Buah Asal Sulawesi Berdasarkan Morfometri (THE MORPHOMETRIC IDENTIFICATION OF CELEBES FRUIT BATS)

    OpenAIRE

    Tiltje Andretha Ransaleleh; Rarah Ratih Adjie Maheswari; Purwantiningsih Sugita; Wasmen Manalu

    2013-01-01

    The bat is very important for human life, because of their  role as pollinator of plants, as a producer oforganic fertilizer,  and as food.  In Northern Celebes fruit eating bats serve as exotic food, so the presenceof bats were threatened to be extinct due to uncontrolled hunting.  The changes of the forest for plantationlands, damage the habitats and the bats were forced to migrate out.   The aim of the study was to identifythe fruit eating bats of Celebes.  Morphometry of body size, skull,...

  12. PENGELOMPOKAN BERBAGAI MERK MI INSTAN BERDASARKAN KEMIRIPAN KANDUNGAN GIZI DENGAN MENGGUNAKAN ANALISIS BIPLOT

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    AGUSTINUS ANGELAUS ETE

    2014-08-01

    Full Text Available At this time, almost everyone once to consume instant noodles. The high interest of public on the instant noodles should be balanced with enough knowledge about the noodles and its nutritional content, either on it’s instant noodles which have similar nutrient content and nutrient content that become identifier of each this group of noodles. The method can be used to obtain information on several brands of instant noodles that have similar nutrient content and nutrient content type that become identifier of each group of instant noodles is biplot analysis. Biplot analysis can show mie and nutrient content types simultaneously in a two-dimension plot. So that from a plot shows noodles and nutritional content types simultaneously, so that obtain information about the instant noodle that have similar nutrient content and nutrient content types into identifier of each group of instant noodles. This study was used 33 brands of instant noodles as observed objects with the type of nutrient content were observed there were nine. This study aims to find out some instant noodles that have similar nutrient content and nutrient content type that become identifier of each group of instant noodles. From the biplot analysis, obtained six groups of instant noodles with different identifier variables.

  13. METODE PENENTUAN VOLUME AGGREGAT PONDASI JALAN BERDASARKAN VARIASI DAN KESALAHAN: STUDI KASUS

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    Marsinta Simamora

    2012-08-01

    Full Text Available Variations between plans and reality can hardly be avoided in construction projects. These variations may beaccepted when they are still in the acceptance limits, or rejected when they are beyond the acceptance limits.The former is called variation in acceptance and the later is called error. Distinguishing these two in acompleted construction work is very important, as rejected variations (errors may be considered as breaching acontract, thus may have legal implications. The research aims to evaluate the traditional calculation method(MeTrad and to promote variation acceptance and error approach (MeVE that is able to draw a clear linewhether a particular work to be accepted and rejected. The research was conducted by comparing the results ofcalculation of the aggregate volume between MeTrad and MeVE. The data was collected from Mauponggo-Puuwada road project in Nangakeo in 2010. The results show that there was no difference in the volumescalculated using both methods. The volume of Class B aggregate based on MeTrad calculation is 386.01m 3andthe volume based on MeVE is 386.01m3; where 16.67m3 of which (3.34% of the planned volume is in the rangeof acceptance and 369.34m3 of which (73.94% of the planned volumeis the volume that is rejected (error. Theresults suggest that MeTrad cannot distinguish clearly whether the work is within the limits of acceptance orrejection, while on the other hand MeVE can describe in detail parts of the work to be accepted or rejected.Using this MeVE calculation, an assessment of a construction workis expected to be more objective anddetailed.

  14. Akumulasi Logam Berat (Cu Dan Pb) Pada Rhizophora Stylosa Berdasarkan Tingkat Pancang Dan Pohon

    OpenAIRE

    Siburian, Fitri

    2015-01-01

    Industrial activities, community and sea transport cause waste containing heavy metals that cause pollution to the mangrove ecosystem and degrade water quality.The purposes of this research were to analyzed the content of heavy metals Cu and Pb on roots, leaves, the bark of R. stylosa and to knew the ability of R. stylosa on accumulate heavy metals. The sample was located in Nelayan Village sub-district Medan Labuhan. Analysis of heavy metals Cu and Pb was carried out in a Laboratory Resear...

  15. PERLINDUNGAN HAM BURUH MIGRAN TAK BERDOKUMEN BERDASARKAN HUKUM PERDAGANGAN DAN HUKUM HAM INTERNASIONAL

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    Sefriani

    2013-02-01

    Full Text Available Undocumented migran worker violate immigration rule and potentially give damage toward host state. However many persons exploited them, get advantages from illegal status of the worker. Two problems in this research are how does international law protect undocumented migrant worker and what solutions offer to host state to solve undocumented migran worker matter. This thesis employs normative method of research with qualitative analysis. The result of this thesis reveals international law protect undocumented worker without discrimination. Preventive action will be better conducted by host state than enforcement

  16. Analisa Layout Surat Kabar Berdasarkan Prinsip-Prinsip Desain Melalui Metode Estetika Birkhoff

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    Arik Kurnianto

    2013-09-01

    Full Text Available This study has primary focus to analyze the aesthetic layout of a newspaper with a mathematical approach, which refers to some methods of Birkhoffs aesthetic measurement developed by David Ngo Chek Ling, et.al. Ngo developed a method of measuring the aesthetic layout that can be used for various design purposes, especially for a computer interface design. Measurement method that he developed refers to the principles of design aesthetics, such as: the principle of balance, the principle of sequence, the principle of unity, and the principle of equilibrium. Birkhoff aesthetic measurement method was used as final analysis to determine the value of aesthetic layout. In this study, some national and local media were selected as samples to determine and assess the aesthetic layout. Based on data analysis, Birkhoff aesthetic measurement method can be applied to measure the aesthetic value of the newspaper layout mathematically.

  17. IDENTIFIKASI FATOR-FAKTOR RISIKO STRESS DAN VARIABLE SOSIODEMOGRAFI BERDASARKAN SURKESDA NANGGROE ACEH DARUSSALAM 2006

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    Sri Idaiani

    2012-07-01

    Full Text Available Nanggroe Aceh Darussalam (NAD is a region which has faced protracted conflict following by tsunami disarter on December, 26 2004. Surkesda NAD 2006, as post tsunami health survey, was conducted in the middle of 2006 which included Self Reporting Questionnaire (SRQ to assess mental health problem in NAD province. The identification of stress factors and socio demographic variables in NAD is a further analysis based on SRQ from Surkesda NAD 2006. The analysis covered 1210 people aged 15 years and over spread out in 21 distiricts/cities in NAD. The overall prevalence of neurosis in NAD in 2006 was 22,6% with prevalences by districts/cities ranged from 5% to 59%. Two districts with prevalence above 50% were Aceh Singkil and Gayo Lues, while districts/cities with prevalence below 10% belonged to Sabang and Aceh Jaya. Symptoms that most people reported were headache (51,2%, fatique easy (37%, sleep difficulty (26%, abdominal discomfort (25.6% and loss of appetite (21,1%. Female had risk more than one and a half to neurosis (OR=1.598; 95% CI: 1,121- 1,772. Aged ≥ 55 year had risk almost two times to neurosis (OR=1,923; 95% CI: 1,592-2,323. Divorce status had risk 1.7 times (OR=1,73; 95% CI: 1.082-2.767. Living in rural had risk 1.4 times (OR=1,409; 95% CI: 1,155-1,858. Married with low socioeconomic status had risk 1.7 times (OR=1,790; 95% CI: 1,263-2,539. The study suggests that the most important region to get more attention are Aceh Singkil and Gayo Lues districts. The study also recommend of the need of mental health intervention for old group, community mental health that focus on non psychosis mental disorder identification to prevent serious mental disorder, and availability of some medicines to overcome chronic stress symptoms. Keywords: tsunami, mental health, SRQ

  18. Gambaran Efektivitas Kepemimpinan Bina Nusantara Computer Club Kepengurusan Ke-22 Berdasarkan Fiedlers Contingency Model

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    Eleonora Francisca Maria Sumual

    2011-03-01

    Full Text Available Fiedler has theory which is called contingency model. According to him, if an organization attempts to achieve group effectiveness through leadership, then there is a need to assess the leader according to an underlying trait, assess the situation faced by the leader, and construct a proper match between the two. With Fiedler model, researchers were doing assessment to one of the student organization in Bina Nusantara University, which is BNCC 22nd student committee. The result of the research is that student organization in this case two committees from BNCC show two different results. The first one does not fit with Fiedler model, which is the people oriented leader can make an effective leadership, and the second one with people oriented leader seems not effective to lead the committee with more old member of the organization.

  19. POLA ENDAPAN MINERAL DAN UNSUR LOGAM BATUAN DI DAS JENEBERANG BERDASARKAN DATA XRF, XRD DAN AAS

    OpenAIRE

    Muhammad Altin Massinai; Lantu; Makhrani; Maria

    2014-01-01

    Sungai Jeneberang merupakan aliran letusan gunungapi Lompobattang. Erupsi tersebut mengendapkan mineral-mineral di sepanjang aliran sungai Jeneberang, baik dari jenis logam maupun non logam. Indikasi mineralisasi di daerah DAS Jeneberang ditunjukkan dengan ditemukannya kekar-kekar yang berupa urat-urat (veins). Prospek akan mineralisasi ini juga ditunjang oleh struktur geologi yang termasuk cukup kompleks, karena Pulau Sulawesi merupakan pertemuan tiga lempeng bumi yakni Eurasia, Indo-Austra...

  20. Analisis Kandungan Klorin Pada Air Teh Celup Berdasarkan Suhu Dan Waktu Pencelupan Di Kota Medan 2013

    OpenAIRE

    Silaban, Saurma Erni Selvita

    2014-01-01

    Tea is the most consumed beverage either cold or hot. One kind of them is bag tea products. Tea bag is wrapped in smooth porous paper and heat resistant. In general, the paper uses chlorine as a bleach, it is the reason for necessary to study the chlorine content in water of tea bag. The purpose of this study was to determine the chlorine content in water of tea bag based on temperature and time. The kind of this study is descriptive survey. The object of study is the water of tea bag mixi...

  1. Aedes aegypti sebagai Vektor Demam Berdarah Dengue Berdasarkan Pengamatan di Alam

    Directory of Open Access Journals (Sweden)

    M. Hasyimi

    2012-09-01

    Full Text Available Demam berdarah atau Demam Berdarah Dengue (DBD masih merupakan masalah kesehatan masyarakat. Terutama bagi kota-kota besar yang penduduknya padat dan mobilitas tinggi. Nyamuk Aedes aegypti merupakan penyebar utama penyakit ini, di samping Ae. albopictus. Berhubung sampai saat ini penyakit DBD belum ada obatnya dan vaksin untuk pencegahannya maka pemberantasan penyakit DBD ini dipusatkan kepada pengendalian nyamuk penularnya. Metode pengendalian telah dilakukan dengan bermacam-macam cara, baik secar kimiawi maupun bukan kimiawi.

  2. Kategorisasi dokumen web secara otomatis berdasarkan folksonomy menggunakan multinomial naive Bayes classifier

    OpenAIRE

    Irawan, Hendy

    2016-01-01

    Folksonomy is a non-hierarchical document categorizing system, that treats every category in a flat manner, dan every category is entered freely by anyone who submitted a document in these categories. Categorization is done automatically at the time a document is submitted, by entering the list of categories that best fit the document. del.icio.us (http://del.icio.us) site is one of the most popular social bookmarking sites that uses folksonomy. Usage of folksonomy, although very easy, also h...

  3. Nilai Kesukaan Konsumen Terhadap Teh Daun Gaharu (Aquilaria malaccensis Lamk.) Berdasarkan Letak Daun Pada Pohon

    OpenAIRE

    Adriana, Nora

    2015-01-01

    Agarwood is a product of Non-Timber Forest Products are of high value and are exported to foreign countries. Utilization of wood still focused on the use of agarwood trees, sap and skin is more often used as a distinctive aroma produced. Therefore, it is necessary to study on the effect of the aloe plant leaf lies on the characteristics of the value of consumer preferences in the tea leaves of the aloes that dihasilkan.Tujuan this study is to determine the level of preference for the public t...

  4. Nilai Kesukaan Konsumen Terhadap Teh Daun Gaharu (AquilariamalaccensisLamk) Berdasarkan Letak Daun Pada Pohon

    OpenAIRE

    Adriana, Nora

    2016-01-01

    Agarwood is a product of non-timber forest product are high value and are exported to foreign countries. Utilization of wood still focused on the use of agarwood tress, sap and skin is more often used as a distinctive aroma produced. Therefore, it is necessary to study on the effect of the aloe plant leaf leaves of that aloes. The purpose of this study is to determine the level of preference for the public to tea agarwood (A. malaccensis Lamk) based on the location of the leaves on the stem. ...

  5. Uji Antioksidan Daun Muda dan Daun Tua Gaharu (Aquilaria malaccensis Lamk) Berdasarkan Perbedaan Tempat Tumbuh Pohon

    OpenAIRE

    Harahap, Rizki Khadijah

    2016-01-01

    The leaf of aloe has an antiokxidant activity which can reduce free radicals. The research is done to know the class of chemical compounds and to know the effect of grown ground of Aloe toward chemical compund of fresh and rotten leaf, and its antioxidant activities. Ethanol extractr the leaf of Aloe by using maceration method, concentrated by rotary evaporator and evaporated by waterbath. The test of antioxidant activity use DPPH method 1,1 – diphenil-2-picrylhydrazil (DPPH), and an observed...

  6. KARAKTERISASI DAN JARAK KEMIRIPAN UWI (Dioscorea alata L. BERDASARKAN PENANDA MORFOLOGI UMBI

    Directory of Open Access Journals (Sweden)

    Trimanto Trimanto

    2012-01-01

    Full Text Available Yam (Dioscorea alata L. is a potential local crop for supporting food security program in Indonesia.  Inventory and characterization of yam have been conducted in scattered population at Nganjuk region - East Java.  The research was aimed to identify the characteristic of Nganjuk’s yam, based on the tuber morphology and to determine its similarity distance value.  The characterisation were analyzed using interval similarity function, based on NTSys DICE coefficient programme. The result showed that 22 accession numbers which comprises of 13 variants of yam were found in Nganjuk region. The same variants of yams indicated by their close similarity distance although they were originated from different areas.

  7. PENGENALAN KEPRIBADIAN SESEORANG BERDASARKAN SIDIK JARI DENGAN METODE FUZZY LEARNING VECTOR QUANTIZATION DAN FUZZY BACKPROPAGATION

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    I Gede Sujana Eka Putra

    2014-12-01

    Full Text Available Kepribadian dapat diidentifikasi melalui analisis pola sidik jari. Pengenalan kepribadian umumnyamenggunakan uji psikometri melalui serangkaian tahapan yang relatif panjang. Melalui analisis pola sidik jari, dapatdiidentifikasi kepribadian secara lebih efisien. Penelitian ini mengajukan algoritma klasifikasi Fuzzy LearningVector Quantization (Fuzzy LVQ karena waktu komputasi yang lebih cepat dan tingkat pengenalan yang tinggi, dandengan metode Fuzzy Backpropagation yang mampu menyelesaikan model data non linier. Tahapan penelitianterdiri dari akuisisi dan klasifikasi. Tahapan pertama melalui akuisisi sidik jari, ekstraksi fitur, proses pelatihan, danpre-klasifikasi. Selanjutnya tahap klasifikasi, melalui klasifikasi fitur sidik jari uji menggunakan algoritma FuzzyLVQ, dibandingkan dengan Fuzzy Backpropagation. Kepribadian diidentifikasi melalui pola hasil klasifikasimenggunakan basis pengetahuan dermatoglyphics. Unjuk kerja diukur dari pencocokan pola hasil pre-klasifikasidan hasil klasifikasi. Hasil penelitian menunjukkan klasifikasi Fuzzy LVQ tingkat kecocokan tertinggi 93,78%dengan iterasi pelatihan maksimum=100 epoh pada target error 10-6. Sedangkan Fuzzy Backpropagation dengantingkat kecocokan tertinggi 93,30% dengan iterasi maksimum diatas 1000 epoh pada target error 10-3. Hal inimenunjukkan Fuzzy LVQ memiliki unjuk kerja lebih baik dibandingkan Fuzzy Backpropagation. Survey respondendilakukan untuk menguji kesesuaian analisa kepribadian sistem dibandingkan dengan kepribadian responden, danhasil survey menunjukkan analisa kepribadian sistem sebagian besar cocok dengan kepribadian responden.

  8. Hubungan Kekerabatan Amfibi (Ordo Anura) di Kecamatan Sibolangit Kabupaten Deli Serdang Berdasarkan Morfometrik

    OpenAIRE

    Ginting, Junaydy Michael Angelo

    2016-01-01

    The research of Amphibians (order Anura) relationship based on morphometrics in Sibolangit, Deli Serdang District, North Sumatera has been conducted in January to July 2015. The purpose of this research was to determine the types and descriptions of Anura species, morphometrics diversity and morphometrics relationship of Anura. Study site was divided into seven different locations settled using Purposive Random Sampling Method. This study was conducted by the active search (...

  9. Perbedaan kualitas hidup anak penderita penyakit ginjal kronis berdasarkan stadium penyakit ginjal kronis

    OpenAIRE

    Nadeak, Dicky Iskandar

    2014-01-01

    Background Chronic kidney disease (CKD) is a condition whereas kidney damage occurs at least 3 months with or without decrease of glomerular filtration rate or glomerular filtration rate less than 60mL/minute/1.73m¬2. Assessment quality of life is important in children with CKD cause it can influence physical growth, developmental retardation, cognitive impairment, academic imparment, emotional disorders and social relationships Objective To assess quality of life in children based on stag...

  10. KARAKTERISASI DAN JARAK KEMIRIPAN UWI (Dioscorea alata L.) BERDASARKAN PENANDA MORFOLOGI UMBI

    OpenAIRE

    Trimanto Trimanto

    2012-01-01

    Yam (Dioscorea alata L.) is a potential local crop for supporting food security program in Indonesia.  Inventory and characterization of yam have been conducted in scattered population at Nganjuk region - East Java.  The research was aimed to identify the characteristic of Nganjuk’s yam, based on the tuber morphology and to determine its similarity distance value.  The characterisation were analyzed using interval similarity function, based on NTSys DICE coefficient programme. The result show...

  11. Sebaran Lokasi Penanaman Bawang Merah Lokal Samosir Berdasarkan Ketinggian Tempat Di Daerah Tangkapan Air Danau Toba

    OpenAIRE

    Hutapea, Rose Friska

    2016-01-01

    This research aims to determine the location planting distribution of Samosir’s local onion based on altitude in Watershed of Lake Toba. This research was conducted in the central region of onion planting around of Lake Toba which have at altitude 900 – 2000 meters above sea level. This research used survey methods which includes literature studies, preparation of the questionnaire, the determination of research location, location mapping for the research, survey around of Lake Toba and proc...

  12. EVALUASI SISTEM INFORMASI KENDARAAN (GFSERVICE)- BINUS UNIVERSITY BERDASARKAN KERANGKA COBIT PADA DOMAIN DELIVERY AND SUPPORT

    OpenAIRE

    Tangkas Udiono; Haris Setia Budi; Mariyani

    2015-01-01

    Information Systems Audit is an activity that is carried out in a company that aims to ensure compliance with existing policies. General Services is one of the units in BINUS University whose activities in the form of services such as Transportation and Document Courier, Copy Centre and General Facility. However, this study is limited to the Transportation and Document Courier unit that uses an online reservation application gfservice.binus.ac.id. Maturity level of the existing information sy...

  13. Prakiraan Daerah Penangkapan Ikan Di Selat Bali Berdasarkan Data Citra Satelit

    Directory of Open Access Journals (Sweden)

    Iqbal Ghazali

    2011-10-01

    Full Text Available Indonesia has a lot of potential marine ecosystem and fisheries, this condition make some many Indonesian get a occupation to be fisherman. However, that is make a problem during his fishing activity, so we have to make some sophisticated technology to support that is activity. At the time, remote sensing technology is the answer for they problem, it is because of that is technology fisherman can be improvement they catcher with more efficient. Determination of fishly ground area by remote sensing technology has some stage before arranging layout Fishly Ground Area Estimation (FGAE map. Procedure to arranging are respectively data searching, data processing, and data analysis, and the last stage is composing of layout of FGAE map.The main purpose of this study is to know about catching area at Bali straits based on image satellite with creating layout of FGAE map. This activity doing descriptive method and the value are based on observation, interview, active participation, and literature search data. At the PDPI making process, the chlorophyl-a data and sea surface temperature getting important parameter, which temperature is the main to parameter to understand of front area and upwelling which is have a lot of nutrient composition. In the other hand, chlorophyl-a is also parameter to know of prosperity area. The data of current, wind speed, wave, and sea surface level are important component as supporting data that will be help to fisherman on determination of fishing ground area at helping fisherman in catching activity. Keywords: Fishing ground, Remote sensing, Bali straits, Chlorophyl-a, Sea surface temperature  

  14. DAYA SAING PT BENAR FLORA UTAMA BERDASARKAN AKTIVITAS RANTAI NILAI FLORIKULTURA

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    Ratih Marina Kurniaty

    2012-11-01

    Full Text Available ABSTRACTThe aims of this research were (1 to identify of the value chain that has been applied by the company, (2 to describe projections from internal and external condition as input in the company’s success factor analysis, (3 to determine the company’s strategic position, and (4 to formulate alternative strategies to increase the company’s competitiveness in  floriculture industry.  This research used SWOT analysis method by considering company’s position and value chain approach which was started with chain mapping to describe actors involved, what business issues in this company face and which activity has the greatest contribution. The value chain approach were also used to determine the prominent product based on the annual sales report. The research results indicated that the issues in the value chain include: expensive input prices, uncertainty in seed supply and higher transportation cost especially for Euro Country and America. Based on internal and external matrix, the company’s position is in the strategic growth quadrant, so the company can apply some alternative strategies such as  (1 maintaining good relationships with customers and suppliers, (2 expanding market to Asia, (3 upgrading the types of tree, (4 attending big exhibitions, (5 develop products by creating plants trendcentre, (6 enhancing the knowledge management for the company’s human resource, (7 production maximizing for the domestic market, (8 establishing distribution channels in each city, (9 setting a clear target market, (10 maintaining the quality of the plants, (11 restructuring the company in order to clarify the specialization of work, and (12 designing documents to better provide the seeds according to customers’ need.Keywords: competitiveness, floriculture industry, chain mapping, value chain economic analysis, SWOT analysisABSTRAKTujuan penelitian ini adalah (1 identifikasi rantai nilai yang telah diterapkan oleh perusahaan, (2 menggambarkan dari kondisi internal dan eksternal sebagai analisis faktor keberhasilan perusahaan, (3 posisi strategis perusahaan, dan (4 merumuskan strategi alternatif untuk meningkatkan daya saing dalam industri florikultura. Penelitian ini menggunakan metode analisis SWOT dengan mempertimbangkan posisi perusahaan dan pendekatan value chain dimulai dengan pemetaan rantai untuk menggambarkan siapa aktor yang terlibat, apa masalah bisnis di perusahaan ini dan aktivitas mana memiliki kontribusi terbesar. Pendekatan rantai nilai juga digunakan untuk menentukan produk terkemuka dari laporan penjualan tahunan. Hasil penelitian menunjukkan bahwa isu-isu dalam rantai nilai meliputi: harga input yang mahal, pasokan benih tidak menentu dan biaya transportasi terutama untuk Negara Eropa dan Amerika lebih tinggi. Hasil matriks internal dan eksternal, posisi perusahaan pada pertumbuhan strategis sehingga perusahaan menerapkan beberapa strategi. Strategi alternatif yang dapat ditingkatkan oleh perusahaan, seperti: (1 maintaning hubungan baik dengan pelanggan dan pemasok, (2 memperluas pasar ekspor ke Asia, (3 meningkatkan jenis pohon, (4 mengikuti pameran besar, (5 berinovasi produk ke membuat trendcentre tanaman, (6 peningkatan manajemen pengetahuan untuk sumber daya manusia di perusahaan, dan (7 memaksimalkan produksi untuk pasar domestik, (8 saluran distribusi yang didirikan di setiap kota, (9 membuat target pasar yang jelas, (10 mempertahankan kualitas tanaman, (11 merestrukturisasi perusahaan dalam rangka untuk memperjelas spesialisasi pekerjaan, (12 membuat dokumen menyediakan benih sesuai dengan kebutuhan pelanggan.Kata kunci: daya saing, industri florikultura, rantai pemetaan, analisis rantai nilai ekonomi, analisis SWOT

  15. TANGGUNG JAWAB DIREKSI DALAM KEPAILITAN PERSEROAN TERBATAS BERDASARKAN UNDANG-UNDANG PERSEROAN TERBATAS

    Directory of Open Access Journals (Sweden)

    Mr. Kurniawan

    2012-10-01

    Full Text Available In running their duties, the board of directors are given rights and full authority to represent the company as long as they act in conformity to the corporation’s Article of Associations. The directors of a limited liability company have both internal and external responsibilities. Internal responsibility includes the Directors’ liability towards the Company and its Shareholders, meanwhile external responsibility covers the Directors’ liability towards third parties to whom the company owes direct or indirect legal connection. There are two approaches to reason the effect of insolvency order to directors of a limited liability, i.e. ‘by operation of law’ and ‘rule of reason’. Dalam menjalankan tugasnya, direksi diberikan hak dan kekuasaan penuh mewakili perseroan, sepanjang bertindak sesuai dengan Anggaran Dasar perseroan. Tanggung jawab direksi perseroan terbatas terdiri dari tanggung jawab yang bersifat internal dan eksternal. Tanggung jawab internal meliputi tanggung jawab Direksi terhadap Perseroan dan Para Pemegang Saham, sedangkan tanggung jawab eksternal berupa tanggung jawab Direksi terhadap pihak ketiga yang berhubungan hukum dengan Perseroan, baik langsung maupun tidak langsung. Terdapat 2 (dua model pemberlakuan akibat hukum pernyataan pailit direksi perseroan terbatas, yaitu akibat berlaku demi hukum dan akibat berlaku secara rule of reason.

  16. Keragaman Genetik Tiga Populasi Kelapa Sawit (Elaeis guinensis Jacq.) Tipe Pisifera Berdasarkan Marka RAPD

    OpenAIRE

    Prayogi, Heru

    2015-01-01

    Palm oil as a plant producing oil is one of the primadonna oil palm plantations that become a source of producer non-migas for Indonesia foreign exchange. Superior varieties of palm oil produced by various research institutions that is a hybrid is tenera dura x pisifera (DxP). Pisifera having an important function in the production of seed oil palm. Hence the population pisifera very important to be managed and developed . A method of RAPD is a method to identify a number of large polymorphis...

  17. Desain Routing Information Protocol pada Jaringan Kamputer dengan Pengalokasian Jumlah Host Per Jaringan Berdasarkan VLSM

    OpenAIRE

    Lubis, Mhd Abdi Wahyuda

    2015-01-01

    The allocation of IP addresses is fundamental in building a computer network. In order for IP address allocation can be efficiently applied to each device connected to a network, first we should calculate the block of IP addresses available. On the other hand the process of routing in the network also requires efficient allocation of IP addresses. To help determining the allocation of IP address and routing path, an application as alternative solution is required. This study uses the Routing ...

  18. Pendugaan Umur Simpan Kerupuk Bawang Kentang Dengan Metode Akselerasi Berdasarkan Pendekatan Kadar Air Kritis

    OpenAIRE

    Murni, Asri

    2016-01-01

    The aim of this research was to find the effect of several types of packaging and storage time of the onion potato chips, and to estimate the shelf life of onion potato chips which were packed in polypropylene, polyethylene, and metalized plastic using accelerated method based on the critical moisture content approach. The effect of the types of packaging and storage time of the onion potato chips was determined using a completely randomized design with two factors,i.e.packaging type (K) (pol...

  19. Proses Kliring Berdasarkan Warkat Pada Bank Mandiri Cabang Medan Imam Bonjol

    OpenAIRE

    Gultom, Novita Grezsia

    2014-01-01

    Kliring dilaksanakan di Bank Indonesia (BI) dengan menggunakan SKN (Sistem Kliring Nasional), dimana sistem ini baru diterapkan yang dimana sebelum menggunakan sistem SKN Kliring dilaksanakan dengan menggunakan SOKL (Sitem Otomasi Kliring Lokal). Pada daerah didalam wilayah Indonesia yang tidak ada Bank Indonesia maka proses Kliring dilakukan oleh salah satu Bank yang ditunjuk oleh Bank Indonesia pada daerah tersebut sehingga selain berfungsi sebagai Bank Umum yang melayani nasabah, maka Bank...

  20. Gambaran Efektivitas Kepemimpinan Bina Nusantara Computer Club Kepengurusan Ke-22 Berdasarkan Fiedlers Contingency Model

    OpenAIRE

    Eleonora Francisca Maria Sumual; Juliana Lee

    2011-01-01

    Fiedler has theory which is called contingency model. According to him, if an organization attempts to achieve group effectiveness through leadership, then there is a need to assess the leader according to an underlying trait, assess the situation faced by the leader, and construct a proper match between the two. With Fiedler model, researchers were doing assessment to one of the student organization in Bina Nusantara University, which is BNCC 22nd student committee. The result of the researc...

  1. Pola Spatial Persebaran Pusat Perbelanjaan Modern Di Surabaya Berdasarkan Probabilitas Kunjungan

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    Achmad Miftahur Rozak

    2013-09-01

    Full Text Available Surabaya sebagai kota besar saat ini  mulai dipenuhi dengan pusat perbelanjaan modern hingga mencapai lebih dari 20 unit. Perkembangannya saat ini menyebabkan  adanya persaingan antar pusat perbelanjaan satu dengan yang lain.  Dengan kondisi seperti ini, diperlukan identifikasi mengenai besaran nilai probabilitas masing-masing pusat perbelanjaan terhadap penduduk yang ada di Suarabaya. Dalam penelitian ini, penentuan probabilitas kunjungan didapatkan dari model Huff. Model ini menghitung nilai probabilitas penduduk yang ada terhadap pusat perbelanjaan dengan menggunakan variabel jarak dan luas bangunan total. Hasil perhitungan tersebut kemudian dikombinasikan dengan perkiraan jangkauan pelayanan tiap pusat perbelanjaan sehingga didapatkan tipologi kecamatan terhadap kemungkinan mengunjungi seluruh pusat perbelanjaan yang ada di Surabaya. Didapatkan bahwa terdapat empat kategori yang masing-masing memiliki karakteristik dan variabel yang mempengaruhinya. Kategori pertama merupakan 5 kecamatan dengan probabilitas rendah dan terjangkau dengan faktor yang berpengaruh adalah luas lantai bangunan serta jarak yang digambarkan oleh jangkauan pelayanan. Kategori kedua merupakan 22 kecamatan dengan probabilitas sedang dan terjangkau dengan faktor yang mempengaruhi adalah jangkauan pelayanan serta jarak terhadap masing-masing pusat perbelanjaan. Kategori tiga merupakan 2 kecamatan dengan probabilitas tinggi dan terjangkau dengan faktor yang berpengaruh adalah luas pusat perbelanjaan. Sedangkan kategori terakhir adalah 2 kecamatan dengan probabilitas sedang namun belum terjangkau oleh layanan pusat perbelanjaan

  2. ANALISIS HUBUNGAN PENGGUNAAN OBAT FDC/KOMBIPAK PADA PENDERITA YANG DI DIAGNOSIS TB PARU BERDASARKAN KARAKTERISTIK

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    Suharmiati Suharmiati

    2012-11-01

    Full Text Available Background: Tuberculosis was an infectious disease and remains a public health problem in the world, including Indonesia. Based on the WHO report in 2009, Indonesia was included into the group of high-burden countries, ranks third after India and China. Indonesia in 1993/1994 began using alloys Anti Tuberculosis (TB called Kombipak. Although this alloy has a high effectiveness in the treatment of pulmonary TB smear positive but low recovery rates for allegedly due to low adherence to medical treatment regularly and the number of drugs taken too much. Therefore created a new drug which has basically the same alloy with Kombipak/the FOG (Fixed Dose Combination which can reduce the risk of compliance in treatment and Multi Drug Resistance. Due to the TB treatment. Methods: this study analyzed the use of drugs Kombipak/FDC in patients diagnosed with pulmonary tuberculosis based on the characteristics. This research is a secondary data analysis of Health Survey Research Association (Riskesdas in 2010 in the form of a structured questionnaire is a questionnaire for households and a questionnaire to individuals aged 15 years and over. Data analyzed by univariate and bivariate analysis using chi square and biner logistic regression. Results: This study found 1278 TB patients who TB diagnosis in the past 12 months from total sample of 177.926 people. The results showed pulmonary tuberculosis patients who get Kombipak/FDC was 83, 1% and most of the respondents treated in the health centers. TB patient who got treatment completed for 6 months or more was 55,8% and mostly treated in government hospitals or health centers. The results showed that low education TB patients had a tendency to use kombipak/FDC for > 6 months 0.659 times compared to those having higher education (p < 0.05. In conclusion, there was a significant correlation between the use Kombipak/FDC for > 6 months with the education level of respondents. It is suggested the active roleof health provider should educate TB patients who were low education patients. Key words: tuberculosis, Kombipak/FDC drugs, health facilities, characteristics

  3. Prediksi Kualitias Biodiesel Berdasarkan Komposisi Asam Lemak Bahan Mentah (Minyak-lemak

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    Joelianingsih

    2008-04-01

    Full Text Available The characteristics of biodiesel are similar to diesel fuels, and therefore biodiesel becomes a strong candidate to replace the diesel fuels if the need areses. Biodiesel standard- so called SNI 04-7182-2006 has been approved by the Indonesian National Standardization Agency (BSN. The basic fuel properties for biodiesel are influenced by the fatty acid composition of the feedstock such as the sensity, viscosity, cetane number, heating value and cloud point. Therefore, the value of these properties can be predicted from the fatty acids composition of the feedstock using the blending equations. This work uses pure component data for methyl palmitate, methyl stearate, methyl oleate, and methyl oleate to develop and test blending equations for the prediction of the basic fuel properties. The results from the blending equations are compared with literature values for biodiesel for a number of triglyceride sources such as palm and jatropha oils. Typical average errors are less than 10% for the density, cetane number and healting value. The blending equation for the viscosity and cloud point are suitable only for a speciefed biodiesel.

  4. Tafsir Kritis Privatisasi Berdasarkan Hermeneutika Gadamerian: Kasus Privatisasi PT Telkom dan PT Indosat

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    Ayudia Sokarina

    2011-08-01

    Full Text Available Privatization Under Critical Meaning by Using Gadamerian Hermeneutics: The Case for Privatization of PT. Telkom And PT. Indosat. The objectives of the study are to search critical meanings of privatisation in PT Telkom and PT Indosat. This research is using an interpretive approach and critical analysis by using Gadamerian hermeneutics. The study finds that there is other reality which shows that the Government views privatisation as a tool to achieve economic rents. Privatisation has failed to enable the distribution of ownership. At the same time, there is exploitation of consumers in the form of higher rates (as in the case of PT Telkomsel. As a result of privatisation of PT Telkom and PT Indosat has failed to provide justice and prosperity for the people and state.

  5. Pengajaran Lingkungan Rumah Berdasarkan Kepedulian pada Kehidupan Murid SD Sehari-hari

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    Laurens Kaluge

    2016-02-01

    Full Text Available This study examined the Sidoarjo School Improvement Program (SSIP, after a year in action, emphasizing the teaching improvement based on the current concepts of effective teaching. The Social Studies particularly on home environment was the case of application. There were 19 primary schools involved as the experimental and 15 as the control schools, involving 781 Grade 3 pupils representing the urban, semi-urban, and rural subdistricts. The data were collected by using intelligence test, content-related test, questionnaire for pupils and parents, inventories, and open questionnaires. After controlling for the childs ability (intelligence, gender, parental occupation and education, family supports, self-concept, democratic atmosphere, and locus of control; no difference was found among urban, semi-urban, and rural schools. However, the most striking result was that the SSIP made a difference, the attainments of children at experimental schools were higher than those at the control schools.

  6. Penetapan Kadar Iodium Pada Garam Konsumsi Dengan Metode Iodometri Berdasarkan Standar Nasional Indonesia

    OpenAIRE

    Putri, Sri Armia Aditya

    2011-01-01

    Pembuatan garam di Indonesia adalah dengan sistem penguapan air laut menggunakan sinar matahari (solar energy) diatas lahan tanah, namun ada beberapa daerah yang memproduksi garam dengan cara memasak karena kondisi tanah yang poros yaitu Propinsi Aceh dan Bali. Produktifitas lahan garam tiap daerah tidaklah sama, hal ini sangat dipengaruhi oleh kualitas tanah tersedia, kelembaban udara, kecepatan angin dan sistem teknologi yang digunakan. Perkiraan produksi garam setiap tahun sulit diperk...

  7. PEMODELAN RESISTIVITAS BAWAH PERMUKAAN BERDASARKAN METODE MAGNETOTELLURIK (STUDI DAERAH GUNUNGMERAKSA-TASIM, SUMATERA SELATAN

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    Endar Widi Sugiyo

    2013-11-01

    Full Text Available Geofisika merupakan ilmu yang mempelajari bumi dengan menggunakan prinsip-prinsip fisika, salah satu metode dalam geofisika adalah metode magnetotellurik yang memanfaatkan konsep elektromagnetik. Data magnetotellurik yang diperoleh dari akuisisi di lapangan tidak lepas dari gangguan noise sehingga perlu dilakukan pengolahan data. Tujuan penelitian ini adalah melakukan pengolahan data magnetotellurik sehingga diperoleh model penampang resistivitas secara 2-dimensi di daerah Gunung Meraksa-Tasim, Sumatera Selatan. Metode magnetotellurik merupakan metode eksplorasi geofisika pasif dimana dilakukan pengukuran medan listrik dan medan magnet alami yang berubah-ubah dalam fungsi waktu. Data akuisisi lapangan diolah dengan menggunakan software SSMT 2000 dan MT-Editor serta diinversi dengan menggunakan software WinGLink. Hasil pengolahan data berupa grafik apparent resistivity dan phase dalam fungsi frekuensi. Model resisitivitas terhadap kedalaman diperoleh dari hasil inversi yang menunjukan struktur berupa lipatan, pendugaan patahan dan struktur berlapis-lapis dari resisitivitas bawah permukaan daerah penelitian. Struktur berlapis-lapis disebabkan karena proses pembentukan daerah penelitian yang merupakan daerah cekungan sedimen.

  8. PROFIL KEMATIAN NEONATAL BERDASARKAN SOSIO DEMOGRAFI DAN KONDISI IBU SAAT HAMIL DI INDONESIA

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    Raharni Raharni

    2012-11-01

    Full Text Available Background: Neonatal mortality is the death of an infant who is born alive within 7 days after birth (early neonatal mortality/perinatal, and the death of a baby born alive more than 7 days until approximately 29 days (advanced neonatal mortality. Neonatal deaths (infants aged 28 days is two thirds of infant mortality, whereas early neonatal mortality/perinatal (infant age of 7 days is two thirds of neonatal deaths. The purpose of this study was to determine neonatal mortality profiles based on socio demografic and the mother condition during pregnancy, and the data based on Riskesdas 2010. Methods: This study used cross sectional design, using data Riskesdas 2010. Result: From the analysis obtained the following results, 144 out of 163 neonatal deaths (88,6% were the early neonatal deaths (7 days of birth, the remain at 11.45% were advanced neonatal mortality. Most of neonatal deaths occur in fertil maternal age at delivery was mature enough that age group 18-34 years, but mostly with low and medium level of education that is 53% and 43% respectively. Mostly neonatal deaths occur when 2 continous birth delivery happens less than 12 months, total number is 100 (61 3%. Percentage of neonatal mortality is equal between working mothers and non working mothers. From the baby's weight with weight < 2500 grams and > 2500 grams, the percentage is nearly equal at 27-29%. Conclusion: The body weight most neonatal deaths were unknown was no significant difference between early neonatal and late neonatal. There demographic information according to both maternal, infant characteristics, or economic status. It is expected the results of this reseach can be used as reference related neonatal mortality profile and as an input in policy to improve the mother health during maternity and birth delivery and also to reduce the risk of neonatal death.   Keywords: early neonatal mortality/perinatal, neonatal mortality, pregnancy

  9. Penilaian Kinerja PT Bank X dalam Mengelola Asset dan Kewajiban Berdasarkan Analisis Resiko dan Analisis Return

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    Muhammad Yusuf

    2010-04-01

    Full Text Available Performance financial evaluation became a primary indicator for management, especially a bank. This research evaluated asset and liability management of PT Bank X to describe its risk and return, by using financial ratios. The results found that performance ratios of PT Bank X decreasingly, where interest margin and net margin decreased to negative. This because of interest expenses and other operational expenses increased. Risk evaluation based on liquidity ratio were also decreasing in 2006 2007, because of the most assets of the bank became illiquid assest and default credits. On the other hand, this conditions became worse because of Rush, many of their customers took their money out from the bank on the same period. In 2008, bank has reduced their default credits successively and made its liquidity better than previous year. Bad quality of credit management and control made the bank budgets their bad allowances for default credits higher and made banks capital risk higher too. Finally, all of banks capital was financed with their liabilities. The other hand, banks operating risk were recovered slowly, by organization rationalization to reduce number of employees, as efficiency also. But this effort, indeed, made employee expenses increasing because management must pay golden-shake hand costs to employees.

  10. Penilaian Kinerja PT Bank X dalam Mengelola Asset dan Kewajiban Berdasarkan Analisis Resiko dan Analisis Return

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    Muhammad Yusuf

    2010-05-01

    Full Text Available Performance financial evaluation became a primary indicator for management, especially a bank. This research evaluated asset and liability management of PT Bank X to describe its risk and return, by using financial ratios. The results found that performance ratios of PT Bank X decreasingly, where interest margin and net margin decreased to negative. This because of interest expenses and other operational expenses increased. Risk evaluation based on liquidity ratio were also decreasing in 2006 – 2007, because of the most assets of the bank became illiquid assest and default credits. On the other hand, this conditions became worse because of “Rush”, many of their customers took their money out from the bank on the same period. In 2008, bank has reduced their default credits succesively and made its liquiditiy better than previous year. Bad quality of credit management and control made the bank budgets their bad allowances for default credits higher and made bank’s capital risk higher too. Finally, all of bank’s capital was financed with their liabilities. The other hand, bank’s operating risk were recovered slowly, by organzation rationalization to reduce number of employees, as efficiency also. But this effort, indeed, made employee expenses increasing because management must pay golden-shake hand costs to employees. 

  11. Tinjauan Atas Penyajian Laporan Keuangan Pemerintahan Kabupaten Asahan Berdasarkan PP No.24 Tahun 2005.

    OpenAIRE

    Manurung, Dina Erika

    2011-01-01

    The goal of paper is to analyze suitability of financial report preparation of District Asahan Government with the law applies transparancy, accountability and participated in management of the local financial. The analyze method used is descriptive, that is to collect, arrange, interprete and analyze the data for giving description and clear answer and accurate from formulation of problem, and then to draw conclusion and suggestions considered to be important. The writer takes data such as f...

  12. Analisis keragaman genetik manggis (Garcinia mangostana diiradiasi dengan sinar gamma berdasarkan penanda ISSR

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    ALFIN WIDIASTUTI

    2013-05-01

    Full Text Available Widiastuti A, Sobir, Suhartanto MR. 2013. Genetic variability analysis of mangosteen (Garcinia mangostana irradiated by gamma ray based on ISSR marker. Bioteknologi 10: 15-22. The aim of the research was to know the increasing of genetic variation within mangosteen based on banding pattern of ISSR marker as a response to some doses of irradiation gamma rays. Five dosages of irradiation gamma rays were 0 Gy, 20 Gy, 25 Gy, 30 Gy, 35 Gy and 40 Gy. The source of the seed that have been irradiated was from Cegal, Leuwiliang, Bogor. The results showed that gamma ray irradiation affecting mangosteen genetic changed. Because the randomize of gamma irradiation, it changed was individually. Gamma irradiation successfully increase genetic variability (5%.

  13. Analisis keragaman genetik manggis (Garcinia mangostana) diiradiasi dengan sinar gamma berdasarkan penanda ISSR

    OpenAIRE

    ALFIN WIDIASTUTI; SOBIR; MUH. R. SUHARTANTO

    2013-01-01

    Widiastuti A, Sobir, Suhartanto MR. 2013. Genetic variability analysis of mangosteen (Garcinia mangostana) irradiated by gamma ray based on ISSR marker. Bioteknologi 10: 15-22. The aim of the research was to know the increasing of genetic variation within mangosteen based on banding pattern of ISSR marker as a response to some doses of irradiation gamma rays. Five dosages of irradiation gamma rays were 0 Gy, 20 Gy, 25 Gy, 30 Gy, 35 Gy and 40 Gy. The source of the seed that have been irradiate...

  14. KUALITAS MIKROBIOLOGI NASI JINGGO BERDASARKAN ANGKA LEMPENG TOTAL, COLIFORM TOTAL DAN KANDUNGAN ESCHERICHIA COLI

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    Ni Luh Payastiti Yunita

    2010-06-01

    Full Text Available Inadequate quality of food products that do not meet standard quality may lead to food poisoning cases and become a concern in public health sector. Nasi Jinggo is a small amount of cooked rice with little bit fried tempe, shredded spicy chicken, traditional made chili sauce that is packed with banana leaves, and all the cooking process usually done traditionally. Nasi jinggo is sold at night by street vendor with price ranged from Rp. 1.500,- to Rp. 2.000,- per package. The study was a quantitative descriptive study with cross-sectional design that was aimed to identify the microbiology quality of nasi jinggo that were sold in Denpasar Selatan area. From 48 population of nasi jinggo street vendor, 23 vendor was taken as samples. The microbiology quality being assessed was Total Plate Count (TPC, Total Coliform with MPN method and Escherichia coli content with Eosin Methylene Blue Agar as medium. Laboratory analysis showed that only 21,7% sample that met TPC standard with average value of 2,3 x 107 CFU/gram sample, only 8,7% that met Total Coliform standard with average value of 1,4 x 105 MPN/100 gram sample and 52,2% sample that met E. coli content standard with average value is 2,6 x 102 MPN/100 gram sample. It is recommended to the government to do quality check towards nasi jinggo in a regular basis as well as to provide education and assistance to nasi jinggo street vendors in order to improve the microbiology quality of food product and ensure food safety to the consumer.

  15. KONSEP HAK SESEORANG ATAS TUBUH DALAM TRANSPLANTASI ORGAN BERDASARKAN NILAI KEMANUSIAAN

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    Hwian Christianto

    2011-02-01

    Full Text Available The idea of right to body autonomy grows more important as people are able to donate their organs. However, the two conceptions of rights currently applicable, i.e. ‘ownership’ and ‘possession’, are highly inappropriate to be applied to this case because human body is not a property. In this light, humanity purposes constitute stronger concept of bodyautonomy. Pemahaman akan hak atas tubuh menjadi semakin penting ketika seseorang dapat mendonorkan organ tubuhnya. Kedua konsep hak yang selama ini berlaku, yaitu konsep “ownership” dan “possession” ternyata jika diterapkan ke hak atas tubuh sangat tidak sesuai karena tubuh manusia bukanlah barang. Dalam kondisi inilah konsep hak dengan tujuan kemanusiaan memberikan dasar yang kuat tentang hak atas tubuh.

  16. Penciptaan Naskah Drama Ambu Hawuk Berdasarkan Tradisi Lisan dan Perspektif Jender

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    Budi Darma

    2013-11-01

    Full Text Available Artikel ini membahas proses penciptaan naskah drama Ambu Hawuk . Naskah ini diadaptasi dari cerita lisandan mitologi lokal yang berkembang di daerah Sunda. Cerita lisan dikumpulkan melalui wawancara mendalamdengan para informan yang mewakili berbagai versi. Setidaknya terdapat enam versi tradisi lisan mengenai tokohini, yaitu versi Cikawungading, Manonjaya, Cianjur, Sukaraja, Parungponteng, dan Galunggung. Dari keenamversi tersebut terdapat variasi dan kesamaan. Naskah ini mencoba membangkitkan kembali semangat feminismedalam peristiwa-peristiwa yang dijalin dalam legenda Ambu Hawuk. Sistem patriarki masyarakat Sunda lebih seringmemandang perempuan sebagai sebuah kaum yang memiliki keterbatasan dibandingkan dengan kaum laki-laki.Cerita ini menggambarkan seorang perempuan yang dianggap berada di belakang laki-laki dan menjadi bayangbayangseorang laki-laki, muncul menjadi seorang pemimpin.Kata kunci: Ambu Hawuk, naskah drama, tradisi lisan.ABSTRACTThe Creating Process of Drama Script Ambu Hawuk Based on the Oral Tradition and the GenderPerspective. This article discusses about the creating process of the drama script Ambu Hawuk. This script was adaptedfrom the oral story and the local mythology developed in Sunda. This oral story was compiled through the intensive interviewwith some sources of information that represented many kinds of versions. There are six versions of the oral traditiontalking about this character, namely: the version of Cikawungading, Manonjaya, Cianjur, Sukaraja, Parungponteng, and Galunggung. Of those six versions, there are varieties and similarities. This script tries to develop feminism spirit on someinterrelated events in Ambu Hawuk legend. From the perspective of Sundanese’ point of view on the patriarchy system, women are regarded as God’s creature who have more basic physical and mental limitations than men. This story describes a woman who is regarded as a person behind the man’s existence and becomes the man’s shadow; but soon afterwards, she becomes a leader.Keywords: Ambu Hawuk, Drama Script, Oral Tradition

  17. PEMBELAJARAN BERDASARKAN MASALAH MATERI GEOMETRI NON EUCLIDES UNTUK MELATIHKAN BERPIKIR KRITIS DAN KREATIF

    OpenAIRE

    Adamura, Fatriya; Masfingatin, Titin; Kirbiana, Elma Puspita

    2016-01-01

    Development and experiment research have been done because there is no learning tools ofproblem based learning. Development research was done by using modified 4-D model that consists of define, design, and develop. Research subject is student in Mathematics Education programme of IKIP PGRI Madiun even semester 2013/2014 that is learning Geometry System. Research instrument were validation sheet, reading validation sheet, learning management observation by lecturer sheet, student activity obs...

  18. STRUKTUR KONSEP MAHASISWA CALON GURU TENTANG MEDAN ELEKTROSTATIK BERDASARKAN ANALISIS FRAMING

    OpenAIRE

    S. E Nugroho; A. Setiawan; - Liliasari

    2012-01-01

    Penelitian ini bertujuan untuk mengeksplorasi struktur konsep mahasiswa calon guru tentang medan elektrostatik. Data diperolehdengan menggunakan metode thinks-aloud, didukung metode lain yaitu wawancara, diskusi, dan tes tertulis Responden penelitianterdiri atas delapan mahasiswa semester 2 yang telah menempuh fisika dasar sebagai kelompok I, dan sembilan mahasiswasemester 6 yang telah menempuh mata kuliah kelistrikan dan kemagnetan sebagai kelompok II. Mahasiswa tersebut diberipertanyaan rea...

  19. Perkiraan Fatigue Life pada Bracket Kapal Tanker Berdasarkan Common Structural Rules

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    Dita septiana

    2012-09-01

    Full Text Available Umur kelelahan (fatigue life dari struktur kapal dianalisis dengan menggunakan standar pada Common Structural Rules for Double Hull Oil Tanker. Kapal tanker single hull direncanakan akan dikonversi menjadi FPSO sehingga perlu diketahui sisa fatigue life konstruksinya. Dalam penelitian ini, fokus analisis dilakukan pada konstruksi bracket karena bracket sebagai salah satu penopang yang esensial pada kapal ini. Oleh karena itu, perlu diketahui letak bracket dengan tegangan paling besar dan besar fatigue lifenya. Kapal tersebut dimodelkan menggunakan softwareMSC Pastran sebagai pre-processor dan MSC Nastran sebagai processor. Bagian kapal yang dimodelkan adalah 3 ruang muat pada midship. Ruang muat pada bagian tengah dari ketiga ruang muat tersebut adalah ruang muat yang paling besar. Fatigue life yang dianalisa merupakan pengaruh dari beban lingkungan (beban gelombang air laut dan beban internal (beban tangki. Kondisi pembebanan yang dilakukan ada 6 macam load cases sesuai dengan ketentuan CSR. Tegangan pada bracket yang didapat dari pemodelan diambil tegangan yang paling besar untuk dianalisa fatigue lifenya. Hasil perhitungan menunjukkan bahwa bracket dengan tegangan paling besar terjadi pada web frame 7 pada ruang muat tengah dan pada kondisi pembebanan A2 didapat umur kapal yang paling rendah yaitu sebesar 26 tahun.

  20. Tingkat Kelelahan Pengemudi Bus Rapid Tranport (BRT Jakarta Berdasarkan Swedish Occupational Fatigue Index (SOFI

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    Rida Zuraida

    2015-06-01

    Full Text Available Fatigue is a common situation experienced by everyone, but until now a simple measuring tools universally accepted is not exist. Fatigue measurement instrument most widely used is an instrument-based questionnaire. This study used the Swedish Occupational Fatigue Index (SOFI as one of the instrument to measure BRT drivers fatigue. The aim is to evaluate physical mental, and shift work fatigue and provide an overview of fatigue levels experienced by the driver BRT on two different shifts. Hopefully it can be used for driver fatigue management by a company. The study was conducted for two different shifts (morning and afternoon, with a total participants are 30 drivers. Results showed that motivation level of shift 2-driver is lower after they finish their work compared to shift1-driver. In shift 1, generally available time to rest, socializing and hanging out with family is longer than available time for shift 2-driver. Perception of loss of energy after work is also higher in shift 2 compared to shift 1, especially the perception of feeling exhausted. Similar results were obtained in the physical aspect and the physical exertion and physical discomfort. Therefore we can conclude the work shift has affected fatigue level experienced by the driver's BRT, and shift rotation is suggested to help the driver to recuperate.