WorldWideScience

Sample records for astrometric signal profile

  1. Astrometric signal profile fitting for Gaia

    CERN Document Server

    Gai, Dr Mario; Busonero, Dr Deborah

    2010-01-01

    A tool for representation of the one-dimensional astrometric signal of Gaia is described and investigated in terms of fit discrepancy and astrometric performance with respect to number of parameters required. The proposed basis function is based on the aberration free response of the ideal telescope and its derivatives, weighted by the source spectral distribution. The influence of relative position of the detector pixel array with respect to the optical image is analysed, as well as the variation induced by the source spectral emission. The number of parameters required for micro-arcsec level consistency of the reconstructed function with the detected signal is found to be 11. Some considerations are devoted to the issue of calibration of the instrument response representation, taking into account the relevant aspects of source spectrum and focal plane sampling. Additional investigations and other applications are also suggested.

  2. Astrometric Microlensing by Local Dark Matter Subhalos

    CERN Document Server

    Erickcek, Adrienne L

    2010-01-01

    High-resolution N-body simulations of dark matter halos indicate that the Milky Way contains numerous subhalos. When a dark matter subhalo passes in front of a star, the light from that star will be deflected by gravitational lensing, leading to a small change in the star's apparent position. This astrometric microlensing signal depends on the inner density profile of the subhalo and can be greater than a few microarcseconds for an intermediate-mass subhalo (Mvir > 10000 solar masses) passing within arcseconds of a star. Current and near-future instruments could detect this signal, and we evaluate SIM's, Gaia's, and ground-based telescopes' potential as subhalo detectors. We develop a general formalism to calculate a subhalo's astrometric lensing cross section over a wide range of masses and density profiles, and we calculate the lensing event rate by extrapolating the subhalo mass function predicted by simulations down to the subhalo masses potentially detectable with this technique. We find that, although t...

  3. Astrometric microlensing of stars

    NARCIS (Netherlands)

    Dominik, M; Sahu, KC

    2000-01-01

    Because of dramatic improvements in the precision of astrometric measurements, the observation of light centroid shifts in observed stars due to intervening massive compact objects ("astrometric microlensing") will become possible in the near future. Upcoming space missions, such as SIM and GAIA,

  4. Astrometric Redshifts for Quasars

    CERN Document Server

    Kaczmarczik, Michael C; Mehta, Sajjan S; Schlegel, David J

    2009-01-01

    The wavelength dependence of atmospheric refraction causes differential chromatic refraction (DCR), whereby objects imaged at different optical/UV wavelengths are observed at slightly different positions in the plane of the detector. Strong spectral features induce changes in the effective wavelengths of broad-band filters that are capable of producing significant positional offsets with respect to standard DCR corrections. We examine such offsets for broad-emission-line (type 1) quasars from the Sloan Digital Sky Survey (SDSS) spanning 0astrometric information can be used to break degeneracies in photometric redshifts of quasars (or other emission-line sources) and, for extreme cases, may be suitable for determining "astrometric redshifts". On the SDSS's southern equatorial stripe, where it is pos...

  5. The Carnegie Astrometric Planet Search Program

    CERN Document Server

    Boss, Alan P; Anglada-Escude, Guillem; Thompson, Ian B; Burley, Gregory; Birk, Christoph; Pravdo, Steven H; Shaklan, Stuart B; Gatewood, George D; Majewski, Steven R; Patterson, Richard J

    2009-01-01

    We are undertaking an astrometric search for gas giant planets and brown dwarfs orbiting nearby low mass dwarf stars with the 2.5-m du Pont telescope at the Las Campanas Observatory in Chile. We have built two specialized astrometric cameras, the Carnegie Astrometric Planet Search Cameras (CAPSCam-S and CAPSCam-N), using two Teledyne Hawaii-2RG HyViSI arrays, with the cameras' design having been optimized for high accuracy astrometry of M dwarf stars. We describe two independent CAPSCam data reduction approaches and present a detailed analysis of the observations to date of one of our target stars, NLTT 48256. Observations of NLTT 48256 taken since July 2007 with CAPSCam-S imply that astrometric accuracies of around 0.3 milliarcsec per hour are achievable, sufficient to detect a Jupiter-mass companion orbiting 1 AU from a late M dwarf 10 pc away with a signal-to-noise ratio of about 4. We plan to follow about 100 nearby (primarily within about 10 pc) low mass stars, principally late M, L, and T dwarfs, for 10...

  6. GAME: Gamma Astrometric Measurement Experiment

    Science.gov (United States)

    Gai, Mario; Lattanzi, Mario G.; Ligori, Sebastiano; Vecchiato, Alberto

    2008-07-01

    The GAME mission concept aims at the very precise measurement of the gravitational deflection of light by the Sun, by means of an optimised telescope operating in the visible and launched in orbit on a small class satellite. The targeted precision on the γ parameter of the Parametrised Post-Newtonian formulation of General Relativity is 10-6 or better, i.e. one to two orders of magnitude better than the best currently available results. Such precision is suitable to detect possible deviations from the unity value, associated to generalised Einstein models for gravitation, with potentially huge impacts on the cosmological distribution of dark matter and dark energy. The measurement principle is based on the differential astrometric signature on the stellar positions, i.e., based on the spatial component of the effect rather than the temporal component as in the most recent experiments using radio link delay timing. The observation strategy also allows some additional scientific objectives related to other tests of General Relativity and to the study of exo-planetary field, multiple aperture Fizeau interferometer, observing simultaneously two regions close to the Solar limb. The diluted optics approach is selected for achieving an efficient rejection of the scattered solar radiation, while retaining an acceptable angular resolution on the science targets. We describe the science motivation, the proposed mission profile, the possible payload implementation and the expected performance.

  7. Astrometric exoplanet detection with Gaia

    Energy Technology Data Exchange (ETDEWEB)

    Perryman, Michael; Hartman, Joel; Bakos, Gáspár Á. [Department of Astrophysical Sciences, Peyton Hall, Princeton, NJ 08544 (United States); Lindegren, Lennart [Lund Observatory, Lund, Box 43, SE-22100 Sweden (Sweden)

    2014-12-10

    We provide a revised assessment of the number of exoplanets that should be discovered by Gaia astrometry, extending previous studies to a broader range of spectral types, distances, and magnitudes. Our assessment is based on a large representative sample of host stars from the TRILEGAL Galaxy population synthesis model, recent estimates of the exoplanet frequency distributions as a function of stellar type, and detailed simulation of the Gaia observations using the updated instrument performance and scanning law. We use two approaches to estimate detectable planetary systems: one based on the signal-to-noise ratio of the astrometric signature per field crossing, easily reproducible and allowing comparisons with previous estimates, and a new and more robust metric based on orbit fitting to the simulated satellite data. With some plausible assumptions on planet occurrences, we find that some 21,000 (±6000) high-mass (∼1-15M {sub J}) long-period planets should be discovered out to distances of ∼500 pc for the nominal 5 yr mission (including at least 1000-1500 around M dwarfs out to 100 pc), rising to some 70,000 (±20, 000) for a 10 yr mission. We indicate some of the expected features of this exoplanet population, amongst them ∼25-50 intermediate-period (P ∼ 2-3 yr) transiting systems.

  8. Astrometric solar system anomalies

    Energy Technology Data Exchange (ETDEWEB)

    Nieto, Michael Martin [Los Alamos National Laboratory; Anderson, John D [PROPULSION LABORATORY

    2009-01-01

    There are at least four unexplained anomalies connected with astrometric data. perhaps the most disturbing is the fact that when a spacecraft on a flyby trajectory approaches the Earth within 2000 km or less, it often experiences a change in total orbital energy per unit mass. next, a secular change in the astronomical unit AU is definitely a concern. It is increasing by about 15 cm yr{sup -1}. The other two anomalies are perhaps less disturbing because of known sources of nongravitational acceleration. The first is an apparent slowing of the two Pioneer spacecraft as they exit the solar system in opposite directions. Some astronomers and physicists are convinced this effect is of concern, but many others are convinced it is produced by a nearly identical thermal emission from both spacecraft, in a direction away from the Sun, thereby producing acceleration toward the Sun. The fourth anomaly is a measured increase in the eccentricity of the Moon's orbit. Here again, an increase is expected from tidal friction in both the Earth and Moon. However, there is a reported unexplained increase that is significant at the three-sigma level. It is produent to suspect that all four anomalies have mundane explanations, or that one or more anomalies are a result of systematic error. Yet they might eventually be explained by new physics. For example, a slightly modified theory of gravitation is not ruled out, perhaps analogous to Einstein's 1916 explanation for the excess precession of Mercury's perihelion.

  9. Effects of disc asymmetries on astrometric measurements - Can they mimic planets?

    CERN Document Server

    Kral, Quentin; Kennedy, Grant; Souami, Damya

    2016-01-01

    Astrometry covers a parameter space that cannot be reached by RV or transit methods to detect terrestrial planets on wide orbits. In addition, high accuracy astrometric measurements are necessary to measure the inclination of the planet's orbits. Here we investigate the principles of an artefact of the astrometric approach. Namely, the displacement of the photo-centre due to inhomogeneities in a dust disc around the parent star. Indeed, theory and observations show that circumstellar discs can present strong asymmetries. We model the pseudo-astrometric signal caused by these inhomogeneities, asking whether a dust clump in a disc can mimic the astrometric signal of an Earth-like planet. We show that these inhomogeneities cannot be neglected when using astrometry to find terrestrial planets. We provide the parameter space for which these inhomogeneities can affect the astrometric signals but still not be detected by mid-IR observations. We find that a small cross section of dust corresponding to a cometary mass...

  10. Gravitation Astrometric Measurement Experiment (GAME)

    Science.gov (United States)

    Gai, M.; Vecchiato, A.; Ligori, S.; Riva, A.; Lattanzi, M. G.; Busonero, D.; Fienga, A.; Loreggia, D.; Crosta, M. T.

    2012-07-01

    GAME is a recent concept for a small/medium class mission aimed at Fundamental Physics tests in the Solar system, by means of an optimised instrument in the visible, based on smart combination of coronagraphy and Fizeau interferometry. The targeted precision on the γ and β parameters of the Parametrised Post-Newtonian formulation of General Relativity are respectively in the 10-7-10-8 and 10-5-10-6 range, improving by one or two orders of magnitude with respect to the expectations on current or near future experiments. Such precision is suitable to detect possible deviations from the unity value, associated to generalised Einstein models for gravitation, with potentially huge impacts on the cosmological distribution of dark matter and dark energy from a Solar system scale experiment. The measurement principle is based on the differential astrometric signature on the stellar positions, i.e. based on the spatial component of the effect rather than the temporal component as in the most recent experiments using radio link delay timing variation (Cassini). The instrument concept is based on multiple field, multiple aperture Fizeau interferometry, observing simultaneously regions close to the Solar limb (requiring the adoption of coronagraphic techniques), and others in opposition to the Sun. The diluted optics approach is selected for achieving an efficient rejection of the scattered solar radiation, while retaining an acceptable angular resolution on the science targets. The multiple field observation is aimed at cost-effective control of systematic effects through simultaneous calibration. We describe the science motivation, the proposed mission profile, the instrument concept and the expected performance.

  11. The Future of Astrometric Education

    Directory of Open Access Journals (Sweden)

    William van Altena

    2006-01-01

    Full Text Available La Astrometría está preparada para entrar en una era de crecimiento y relevancia sin paralelo debido a la esperada abundancia de datos procedentes de las misiones espaciales SIM y GAIA. Se diseñan modernos telescopios terrestres, como el LSST, que brindaran datos menos precisos, pero de una mayor cantidad de estrellas. El potencial para los estudios de estructura, cinemática y dinámica de nuestra Galaxia, así como de la naturaleza física de las estrellas y de las escalas cosmológicas, no tiene igual en la historia de la astronomía. Es irónico, por lo tanto, que a escala global, se puede obtener una educación completa en astrometría sólo en San Petersburgo y en Paris, mientras que en breve, no se dictaría curso alguno de astrometría en los EEUU. ¿Quien aseguraría el control de calidad astrométrico de JWT, SIM, GAIA, LST, por no hablar de los grandes telescopios basados en tierra tales como, VLT, Gemini, Keck, NOAO, Magellan, LBT, etc.? Proponemos una renovación de la educación universitaria en astrometría con el fin de preparar científicos calificados que permitan maximizar los resultados de las actuales inversiones multimillonarias en instrumentación astronómica. Por un lado, las agencias de financiamiento están proporcionando fondos especiales para estos propósitos, y a su vez, las universidades y los observatorios deben reconocer su responsabilidad de contratar astrometrías altamente calificados aún de entrenar estudiantes y supervisar el instrumental astronómico planificado y existente, de tal manera que se obtengan y analicen datos de gran calidad. Una solución provisional a este problema es proponer la realización de series de escuelas internacionales de verano en Astrometría. El Centro Científico Michelson del proyecto SIM coordinarla una escuela de astrometría en 2005, aún de iniciar este proceso. Se sugiere el equivalente a un programa educativo de un semestre en técnicas astrometrías en carreras

  12. The Astrometric Foundation of Astrophysics

    CERN Document Server

    Høg, Erik

    2014-01-01

    Astrophysical studies require a knowledge of very accurate positions, motions and distances of stars. A brief overview is given of the significance and development of astrometry by ESA's two astrometric satellites, Hipparcos and Gaia, launched in respectively 1989 and 2013. The astrometric foundation of all branches of astronomy from the solar system and stellar systems to compact galaxies, quasars and dark matter is being revolutionized by the observations from these satellites. The future of fundamental astrometry must be considered in a time frame of 50 years, therefore science issues for a Gaia successor mission in twenty years are discussed in an extensive report: "Absolute Astrometry in the Next 50 Years" available at https://dl.dropbox.com/u/49240691/GaiaRef.pdf.

  13. The Future of Astrometric Education

    OpenAIRE

    William van Altena; Magda Stavinschi

    2006-01-01

    La Astrometría está preparada para entrar en una era de crecimiento y relevancia sin paralelo debido a la esperada abundancia de datos procedentes de las misiones espaciales SIM y GAIA. Se diseñan modernos telescopios terrestres, como el LSST, que brindaran datos menos precisos, pero de una mayor cantidad de estrellas. El potencial para los estudios de estructura, cinemática y dinámica de nuestra Galaxia, así como de la naturaleza física de las estrellas y de las escalas cosmológicas, no tien...

  14. Satellite Tracking Astrometric Network (STAN)

    Science.gov (United States)

    Vecchiato, Alberto; Gai, Mario

    2015-08-01

    The possibility of precise orbit tracking and determination of different types of satellites has been explored for at least some 25 years (Arimoto et al., 1990). Proposals in this sense made use mainly of astrometric observations, but multiple tracking techniques combining transfer and laser ranging was also suggested (Guo et al., 2009; Montojo et al., 2011), with different requirements and performances ranging from $\\sim100$~m to tenths of meters.In this work we explore the possible improvements and a novel implementation of a technique relying on large angle, high precision astrometry from ground for the determination of satellite orbits. The concept is based on combined observation of geostationary satellites and other near-Earth space objects from two or more telescopes, applying the triangulation principle over widely separated regions of the sky. An accuracy of a few $10^{-2}$~m can be attained with 1-meter-class telescopes and a field of vied of some arcminutes.We discuss the feasibility of the technique, some of the implementation aspects, and the limitations imposed by atmospheric turbulence. The potential benefits for satellite orbit control and navigation systems are presented, depending on the number and position of the contributing telescopes.We also discuss the possibility that, by reversing the roles of stars and satellites, the same kind of observations can be used for verification and maintenance of astrometric catalogs.

  15. Astrometric Detection of Earthlike Planets

    CERN Document Server

    Shao, Michael; Catanzarite, Joseph H; Edberg, Stephen J; Leger, Alain; Malbet, Fabien; Queloz, Didier; Muterspaugh, Matthew W; Beichman, Charles; Fischer, Debra A; Ford, Eric; Olling, Robert; Kulkarni, Shrinivas; Unwin, Stephen C; Traub, Wesley

    2009-01-01

    Astrometry can detect rocky planets in a broad range of masses and orbital distances and measure their masses and three-dimensional orbital parameters, including eccentricity and inclination, to provide the properties of terrestrial planets. The masses of both the new planets and the known gas giants can be measured unambiguously, allowing a direct calculation of the gravitational interactions, both past and future. Such dynamical interactions inform theories of the formation and evolution of planetary systems, including Earth-like planets. Astrometry is the only technique technologically ready to detect planets of Earth mass in the habitable zone (HZ) around solar-type stars within 20 pc. These Earth analogs are close enough for follow-up observations to characterize the planets by infrared imaging and spectroscopy with planned future missions such as the James Webb Space Telescope (JWST) and the Terrestrial Planet Finder/Darwin. Employing a demonstrated astrometric precision of 1 microarcsecond and a noise ...

  16. Signal Peptidase Enzymology and Substrate Specificity Profiling.

    Science.gov (United States)

    Dalbey, R E; Pei, D; Ekici, Ö D

    2017-01-01

    Signal peptidases are membrane proteases that play crucial roles in the protein transport pathway of bacteria. They cleave off the signal peptide from precursor proteins that are membrane inserted by the SecYEG or Tat translocons. Signal peptide cleavage releases the translocated protein from the inner membrane allowing the protein to be exported to the periplasm, outer membrane, or secreted into the medium. Signal peptidases are very important proteins to study. They are unique serine proteases with a Ser-Lys dyad, catalyze cleavage at the membrane surface, and are promising potential antibacterial drug targets. This chapter will focus on the isolation of signal peptidases and the preprotein substrates, as well as describe a peptide library approach for characterizing the substrate specificity.

  17. New signal processing technique for density profile reconstruction using reflectometry

    Energy Technology Data Exchange (ETDEWEB)

    Clairet, F.; Bottereau, C. [CEA, IRFM, F-13108 Saint-Paul-lez-Durance (France); Ricaud, B. [CEA, IRFM, F-13108 Saint-Paul-lez-Durance (France); CPT UMR 6207, Campus de Luminy, case 907, F-13288 Marseille (France); Briolle, F. [CPT UMR 6207, Campus de Luminy, case 907, F-13288 Marseille (France); CReA, BA 701, F-13306 Salon de Provence (France); Heuraux, S. [IJL-P2M, UMR-CNRS 7198, Universite Henri Poincare, F-54506 Vandoeuvre (France)

    2011-08-15

    Reflectometry profile measurement requires an accurate determination of the plasma reflected signal. Along with a good resolution and a high signal to noise ratio of the phase measurement, adequate data analysis is required. A new data processing based on time-frequency tomographic representation is used. It provides a clearer separation between multiple components and improves isolation of the relevant signals. In this paper, this data processing technique is applied to two sets of signals coming from two different reflectometer devices used on the Tore Supra tokamak. For the standard density profile reflectometry, it improves the initialization process and its reliability, providing a more accurate profile determination in the far scrape-off layer with density measurements as low as 10{sup 16} m{sup -1}. For a second reflectometer, which provides measurements in front of a lower hybrid launcher, this method improves the separation of the relevant plasma signal from multi-reflection processes due to the proximity of the plasma.

  18. The Gamma Astrometric Measurement Experiment (GAME)

    Science.gov (United States)

    Gai, Mario; Vecchiato, Alberto; Ligori, Sebastiano; Fineschi, Silvano; Lattanzi, Mario G.

    2009-08-01

    The GAME mission concept is aimed at very precise measurement of the gravitational deflection of light by the Sun, by an optimized telescope in the visible and launched in orbit on a small class satellite. The targeted precision on the γ parameter of the Parametrized Post-Newtonian formulation of General Relativity is 10-6 or better, i.e. one to two orders of magnitude better than the best current results. Such precision is suitable to detect possible deviations from unity value, associated to generalized Einstein models for gravitation, with potentially huge impacts on the cosmological distribution of dark matter and dark energy. The measurement principle is based on differential astrometric signature on the stellar positions, i.e. on the spatial component of the effect rather than the temporal component as in recent experiments using radio link delay timing. Exploiting the observation strategy, it is also possible to target other interesting scientific goals both in the realm of General Relativity and in the observations of extrasolar systems. The instrument is a dual field, multiple aperture Fizeau interferometer, observing simultaneously two regions close to the Solar limb. The diluted optics approach is selected for efficient rejection of the solar radiation, while retaining an acceptable angular resolution on the science targets. We describe the science motivation, the proposed mission profile, the payload concept and the expected performance from recent results.

  19. Astrometric Solar-System Anomalies

    CERN Document Server

    Anderson, John D

    2009-01-01

    There are at least four unexplained anomalies connected with astrometric data. Perhaps the most disturbing is the fact that when a spacecraft on a flyby trajectory approaches the Earth within 2000 km or less, it often experiences a change in total orbital energy per unit mass. Next, a secular change in the astronomical unit AU is definitely a concern. It is increasing by about 15 cm yr$^{-1}$. The other two anomalies are perhaps less disturbing because of known sources of nongravitational acceleration. The first is an apparent slowing of the two Pioneer spacecraft as they exit the solar system in opposite directions. Some astronomers and physicists are convinced this effect is of concern, but many others are convinced it is produced by a nearly identical thermal emission from both spacecraft, in a direction away from the Sun, thereby producing acceleration toward the Sun. The fourth anomaly is a measured increase in the eccentricity of the Moon's orbit. Here again, an increase is expected from tidal friction ...

  20. Spectro-astrometric Study of HI emission lines from Herbig Ae/Be Stars

    Science.gov (United States)

    Cade Adams, Steven; Brittain, Sean D.; Dougados, Catherine; Benisty, Myriam; Podio, Linda; Whelan, Emma

    2015-01-01

    We present a spectro-astrometric study of the Pa β and Br γ lines from six Herbig Ae/Be stars using NIFS on Gemini North. The goal of this study is to determine the origin of the HI emission lines. By combining the high angular resolution ( 0.1") and intermediate spectral resolution (R~5000) of GEMINI/NIFS we measured the spectro-astrometric signal of the Pa β and Br γ emission lines at the 0.1 mas level. The HAe stars showed no significant spectro-astrometric signal, while the HBe stars did show significant detections. We compare our results to models and discuss the implications for understanding the origin of the HI lines in Herbig Ae/Be stars and their utility for measuring the accretion rate. We also discuss various artifacts in the data and prospects for more sensitive measurements in the future.

  1. URAT: astrometric requirements and design history

    CERN Document Server

    Zacharias, N; Rakich, A; Epps, H

    2006-01-01

    The U.S. Naval Observatory Robotic Astrometric Telescope (URAT) project aims at a highly accurate (5 mas), ground-based, all-sky survey. Requirements are presented for the optics and telescope for this 0.85 m aperture, 4.5 degree diameter field-of-view, specialized instrument, which are close to the capability of the industry. The history of the design process is presented as well as astrometric performance evaluations of the toleranced, optical design, with expected wavefront errors included.

  2. URAT: astrometric requirements and design history

    Science.gov (United States)

    Zacharias, Norbert; Laux, Uwe; Rakich, Andrew; Epps, Harland

    2006-06-01

    The U.S. Naval Observatory Robotic Astrometric Telescope (URAT) project aims at a highly accurate (5 mas), ground-based, all-sky survey. Requirements are presented for the optics and telescope for this 0.85 m aperture, 4.5 degree diameter field-of-view, specialized instrument, which are close to the capability of the industry. The history of the design process is presented as well as astrometric performance evaluations of the toleranced, optical design, with expected wavefront errors included.

  3. Utilizing Astrometric Orbits to Obtain Coronagraphic Images

    CERN Document Server

    Davidson, John M

    2010-01-01

    We present an approach for utilizing astrometric orbit information to improve the yield of planetary images and spectra from a follow-on direct detection mission. This approach is based on the notion-strictly hypothetical-that if a particular star could be observed continuously, the instrument would in time observe all portions of the habitable zone so that no planet residing therein could be missed. This strategy could not be implemented in any realistic mission scenario. But if an exoplanet's orbit is known from astrometric observation, then it may be possible to plan and schedule a sequence of imaging observations that is the equivalent of continuous observation. A series of images-optimally spaced in time-could be recorded to examine contiguous segments of the orbit. In time, all segments would be examined, leading to the inevitable detection of the planet. In this paper, we show how astrometric orbit information can be used to construct such a sequence. Using stars from astrometric and imaging target lis...

  4. Signal Processing and Data Acquisition for Wind Profiler Using Labview

    Directory of Open Access Journals (Sweden)

    Priyank V. Gandhi

    2014-04-01

    Full Text Available This paper presents the design of Wind Profiler using LabVIEW. Wind speed is a useful weather parameter to monitor and record for many applications like shipping, aviation, meteorology, construction etc. Wind observations are crucial importance for general (operational aviation meteorology, and numerical weather prediction. Wind profiler radars are vertically directed pulsed Doppler radars capable of analyzing the back-scattered signals to determine the velocity of air along the beams. Steering the beams typically 15° from zenith, the horizontal and vertical components of the air moment can be obtained. The extraction of zeroth, first and second moments is the key reason for doing all the signal processing. For measurements above 20 km, the 50 MHz frequency band can be used. The paper discusses the issues such as the principle of wind profiler radar, how wind profilers estimate the horizontal wind as a function of altitude in "clear air‟, Doppler Beam Swinging (DBS technique for Wind velocity measurement. Moment calculation and Signal processing of recoded experimental data is performed by LabVIEW code developed in my project.

  5. Mechanisms of the formation of biological signaling profiles

    Science.gov (United States)

    Teimouri, Hamid; Kolomeisky, Anatoly B.

    2016-12-01

    The formation and growth of multi-cellular organisms and tissues from several genetically identical embryo cells is one of the most fundamental natural phenomena. These processes are stimulated and governed by multiple biological signaling molecules, which are also called morphogens. Embryo cells are able to read and pass genetic information by measuring the non-uniform concentration profiles of signaling molecules. It is widely believed that the establishment of concentration profiles of morphogens, commonly referred as morphogen gradients, is a result of complex biophysical and biochemical processes that might involve diffusion and degradation of locally produced signaling molecules. In this review, we discuss various theoretical aspects of the mechanisms for morphogen gradient formation, including stationary and transient dynamics, the effect of source delocalization, diffusion, different degradation mechanisms, and the role of spatial dimensions. Theoretical predictions are compared with experimental observations. In addition, we analyze the potential alternative mechanisms of the delivery of biological signals in embryo cells and tissues. Current challenges in understanding the mechanisms of morphogen gradients and future directions are also discussed.

  6. Wavelet based methods for improved wind profiler signal processing

    Directory of Open Access Journals (Sweden)

    V. Lehmann

    Full Text Available In this paper, we apply wavelet thresholding for removing automatically ground and intermittent clutter (airplane echoes from wind profiler radar data. Using the concept of discrete multi-resolution analysis and non-parametric estimation theory, we develop wavelet domain thresholding rules, which allow us to identify the coefficients relevant for clutter and to suppress them in order to obtain filtered reconstructions.

    Key words. Meteorology and atmospheric dynamics (instruments and techniques – Radio science (remote sensing; signal processing

  7. Enhanced Phosphoproteomic Profiling Workflow For Growth Factor Signaling Analysis

    DEFF Research Database (Denmark)

    Sylvester, Marc; Burbridge, Mike; Leclerc, Gregory

    2010-01-01

    understanding of pathological processes. In our study we create and integrate data on phosphorylations that are initiated by several growth factor receptors. We present an approach for quantitative, time-resolved phosphoproteomic profiling that integrates the important contributions by phosphotyrosines. Methods...... A549 lung carcinoma cells were used as a model and stimulated with hepatocyte growth factor, epidermal growth factor or fibroblast growth factor. We employed a quick protein digestion workflow with spin filters without using urea. Phosphopeptides in general were enriched by sequential elution from...... in order to maximize identification of peptides as well as localization of phosphorylation sites. Results and Conclusions The combination of SIMAC with phosphotyrosine enrichment leads to a significant increase in identification of potential signaling events in growth factor receptor signaling networks...

  8. ESPRI: Astrometric planet search with PRIMA at the VLTI

    Directory of Open Access Journals (Sweden)

    Ségransan D.

    2011-07-01

    Full Text Available The ESPRI consortium will conduct an astrometric survey for extrasolar planets, using the PRIMA facility at the Very Large Telescope Interferometer. Our scientific goals include determining orbital inclinations and masses for planets already known from radial-velocity surveys, searches for planets around nearby stars of all masses, and around young stars. The consortium has built the PRIMA differential delay lines, developed an astrometric operation and calibration plan, and will deliver astrometric data reduction software.

  9. A Search For Stellar-mass Black Holes Via Astrometric Microlensing

    Science.gov (United States)

    Lu, J. R.; Sinukoff, E.; Ofek, E. O.; Udalski, A.; Kozlowski, S.

    2016-10-01

    While dozens of stellar-mass black holes (BHs) have been discovered in binary systems, isolated BHs have eluded detection. Their presence can be inferred when they lens light from a background star. We attempt to detect the astrometric lensing signatures of three photometrically identified microlensing events, OGLE-2011-BLG-0022, OGLE-2011-BLG-0125, and OGLE-2012-BLG-0169 (OB110022, OB110125, and OB120169), located toward the Galactic Bulge. These events were selected because of their long durations, which statistically favors more massive lenses. Astrometric measurements were made over one to two years using laser-guided adaptive optics observations from the W. M. Keck Observatory. Lens model parameters were first constrained by the photometric light curves. The OB120169 light curve is well fit by a single-lens model, while both OB110022 and OB110125 light curves favor binary lens models. Using the photometric fits as prior information, no significant astrometric lensing signal was detected and all targets were consistent with linear motion. The significant lack of astrometric signal constrains the lens mass of OB110022 to 0.05-1.79 M ⊙ in a 99.7% confidence interval, which disfavors a BH lens. Fits to OB110125 yielded a reduced Einstein crossing time and insufficient observations during the peak, so no mass limits were obtained. Two degenerate solutions exist for OB120169, which have a lens mass between 0.2-38.8 M ⊙ and 0.4-39.8 M ⊙ for a 99.7% confidence interval. Follow-up observations of OB120169 will further constrain the lens mass. Based on our experience, we use simulations to design optimal astrometric observing strategies and show that with more typical observing conditions the detection of BHs is feasible.

  10. SignalSpider: Probabilistic pattern discovery on multiple normalized ChIP-Seq signal profiles

    KAUST Repository

    Wong, Kachun

    2014-09-05

    Motivation: Chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing (ChIP-Seq) measures the genome-wide occupancy of transcription factors in vivo. Different combinations of DNA-binding protein occupancies may result in a gene being expressed in different tissues or at different developmental stages. To fully understand the functions of genes, it is essential to develop probabilistic models on multiple ChIP-Seq profiles to decipher the combinatorial regulatory mechanisms by multiple transcription factors. Results: In this work, we describe a probabilistic model (SignalSpider) to decipher the combinatorial binding events of multiple transcription factors. Comparing with similar existing methods, we found SignalSpider performs better in clustering promoter and enhancer regions. Notably, SignalSpider can learn higher-order combinatorial patterns from multiple ChIP-Seq profiles. We have applied SignalSpider on the normalized ChIP-Seq profiles from the ENCODE consortium and learned model instances. We observed different higher-order enrichment and depletion patterns across sets of proteins. Those clustering patterns are supported by Gene Ontology (GO) enrichment, evolutionary conservation and chromatin interaction enrichment, offering biological insights for further focused studies. We also proposed a specific enrichment map visualization method to reveal the genome-wide transcription factor combinatorial patterns from the models built, which extend our existing fine-scale knowledge on gene regulation to a genome-wide level. Availability and implementation: The matrix-algebra-optimized executables and source codes are available at the authors\\' websites: http://www.cs.toronto.edu/∼wkc/SignalSpider. Contact: Supplementary information: Supplementary data are available at Bioinformatics online.

  11. Gamma astrometric measurement experiment -science and implementation

    Science.gov (United States)

    Gai, Mario; Vecchiato, Alberto; Lattanzi, Mario G.; Ligori, Sebastiano; Loreggia, Davide; Fineschi, Silvano

    GAME (Gamma Astrometric Measurement Experiment) is a mission concept taking advantage of astronomical techniques for high precision measurements of interest to Fundamental Physics, and in particular the γ parameter of the Parameterized Post-Newtonian formulation of gravi-tation theories modifying the General Relativity. A space based telescope, looking close to the Solar limb thanks to coronagraphic techniques, may implement astrometric measurements sim-ilar to those performed in the solar eclipse of 1919, when Dyson, Eddington and collaborators measured for the first time the gravitational bending of light. Simulations show that the final accuracy of GAME can reach the 10-7 level. GAME will be a decisive experiment for the understanding of gravity physics, cosmology and the Universe evolution. The observations leading to Dark Matter (e.g. galaxy rotation curves) and Dark Energy (accelerated expansion of the Universe) might be explained with a modified version of General Relativity, e.g. in which the curvature invariant R is no longer constant as in Einstein's equations, i.e. the f (R) gravity theories. A 10-7 level determination of γ will provide stringent constraints on acceptable theories. Also, high precision astrometry makes accessible other appealing measurements, e.g. the light deflection induced by the quadrupole moment of giant planets, like Jupiter or Saturn, and, by high precision determination of the orbits of Mercury and high elongation asteroids, the PPN parameter β. GAME may also carry out measurements on selected astrophysical targets, e.g. nearby, bright stars known to host companions with minimum masses in the planetary/brown dwarf regime, and orbital radii in the 3-7 AU range, which are observed by no other present or planned campaigns. GAME, also thanks to high-cadence, high-precision photometry on transit-ing exoplanet systems, will thus improve on our understanding of the actual mass distribution and multiplicity of sub-stellar companions

  12. Astrometric Positioning of Geostationary Satellites (PASAGE)

    OpenAIRE

    T. López Moratalla; C. Abad; F. Belizón; J. C. Coma; F. J. Montojo; J. L. Muiños; Palacio, J.; Vallejo, M.

    2006-01-01

    Se describen las líneas básicas del proyecto PASAGE del Real Instituto y Observatorio de la Armada (España), cuyo objetivo es obtener efemérides precisas de satélites geoestacionarios mediante observaciones visuales astrometrías desde tierra. A priori, se trata de una técnica más precisa que las utilizadas habitualmente y supondría una nueva e importante aplicación de la astronomía basada en tierra.

  13. Deep Astrometric Standards (DAS) and Galactic Structure

    CERN Document Server

    Platais, I; Zacharias, N

    2005-01-01

    The advent of next-generation imaging telescopes such as LSST and Pan-STARRS has revitalized the need for deep and precise reference frames. The proposed weak-lensing observations with these facilities put the highest demands on image quality over wide angles on the sky. It is particularly difficult to achieve a sub-arcsecond PSF on stacked images, where precise astrometry plays a key role. Current astrometric standards are insufficient to achieve the science goals of these facilities. We thus propose the establishing of a few selected deep (V=25) astrometric standards (DAS). These will enable a reliable geometric calibration of solid-state mosaic detectors in the focal plane of large ground-based telescopes and make a substantial contribution to our understanding of stellar populations in the Milky Way. In this paper we motivate the need for such standards and discuss the strategy of their selection and acquisition and reduction techniques. The feasibility of DAS is demonstrated by a pilot study around the o...

  14. ON THE SOURCE OF ASTROMETRIC ANOMALOUS REFRACTION

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, M. Suzanne [Department of Natural and Environmental Sciences, Western State Colorado University, 128 Hurst Hall, Gunnison, CO 81230 (United States); McGraw, John T.; Zimmer, Peter C. [Department of Physics and Astronomy, University of New Mexico, MSC07 4220, Albuquerque, NM 87131 (United States); Pier, Jeffrey R., E-mail: mstaylor@western.edu [Division of Astronomical Sciences, NSF 4201 Wilson Blvd, Arlington, VA 22230 (United States)

    2013-03-15

    More than a century ago, astronomers using transit telescopes to determine precise stellar positions were hampered by an unexplained periodic shifting of the stars they were observing. With the advent of CCD transit telescopes in the past three decades, this unexplained motion, termed 'anomalous refraction' by these early astronomers, is again being observed. Anomalous refraction is described as a low-frequency, large angular scale ({approx}2 Degree-Sign ) motion of the entire image plane with respect to the celestial coordinate system as observed and defined by astrometric catalogs. These motions, of typically several tenths of an arcsecond amplitude with timescales on the order of 10 minutes, are ubiquitous to ground-based drift-scan astrometric measurements regardless of location or telescopes used and have been attributed to the effect of tilting of equal-density layers of the atmosphere. The cause of this tilting has often been attributed to atmospheric gravity waves, but this cause has never been confirmed. Although theoretical models of atmospheric refraction show that atmospheric gravity waves are a plausible cause of anomalous refraction, an observational campaign specifically directed at defining this relationship provides clear evidence that anomalous refraction is not consistent with the passage of atmospheric gravity waves. The source of anomalous refraction is found to be meter-scale, slowly evolving quasi-coherent dynamical structures in the boundary layer below 60 m above ground level.

  15. On the Source of Astrometric Anomalous Refraction

    Science.gov (United States)

    Taylor, M. Suzanne; McGraw, John T.; Zimmer, Peter C.; Pier, Jeffrey R.

    2013-03-01

    More than a century ago, astronomers using transit telescopes to determine precise stellar positions were hampered by an unexplained periodic shifting of the stars they were observing. With the advent of CCD transit telescopes in the past three decades, this unexplained motion, termed "anomalous refraction" by these early astronomers, is again being observed. Anomalous refraction is described as a low-frequency, large angular scale (~2°) motion of the entire image plane with respect to the celestial coordinate system as observed and defined by astrometric catalogs. These motions, of typically several tenths of an arcsecond amplitude with timescales on the order of 10 minutes, are ubiquitous to ground-based drift-scan astrometric measurements regardless of location or telescopes used and have been attributed to the effect of tilting of equal-density layers of the atmosphere. The cause of this tilting has often been attributed to atmospheric gravity waves, but this cause has never been confirmed. Although theoretical models of atmospheric refraction show that atmospheric gravity waves are a plausible cause of anomalous refraction, an observational campaign specifically directed at defining this relationship provides clear evidence that anomalous refraction is not consistent with the passage of atmospheric gravity waves. The source of anomalous refraction is found to be meter-scale, slowly evolving quasi-coherent dynamical structures in the boundary layer below 60 m above ground level.

  16. Evaluation of a tailored injection profile (TIP) algorithm for uniform contrast-enhanced signal intensity profiles in MR angiography.

    Science.gov (United States)

    Wilson, Gregory J; Maki, Jeffrey H

    2016-12-01

    To evaluate a contrast agent injection method that provides constant magnetic resonance imaging (MRI) signal intensity throughout a contrast-enhanced MR angiography acquisition. A tailored injection profile (TIP) algorithm was developed that used the signal intensity profile from a test bolus as an impulse response function, and predicted the response to various multiphasic injection profiles. Antecubital vein injections were administered via a commercially available multiphasic power injector. The TIP algorithm evaluated the predicted responses and selected the injection that best matched the desired (20-sec plateau) profile. Resulting signal intensity profiles from tailored and standard injection profiles were compared in 20 subjects (10 each). All subjects received a weight-based single-dose (0.1 mmol/kg) of gadoteridol, and abdominal aorta signal intensities were measured at 3T with a time-resolved, thick-slice, 3D spoiled-gradient-echo MR sequence with parameters approximating contrast-enhanced MR angiography. The single-phase, standard injection was injected at 1.6 mL/sec. Full-width at 80% maximum (FW80M) signal intensity was significantly longer for the tailored injection profiles (23.0 ± 2.2 vs. 9.0 ± 4.2 sec; P < 0.01). Concurrently, the profile peak signal intensity was reduced by 19% for the tailored profiles (12.0 ± 3.1 vs. 14.8 ± 2.8 times baseline; P = 0.058), nearly reaching significance. Multiphasic tailored injections from a power injector produced longer signal intensity profiles (156% increase in FW80M) with an accompanying decrease (19%) in peak signal intensity compared to a standard, single-phase injection. J. Magn. Reson. Imaging 2016. J. Magn. Reson. Imaging 2016;44:1664-1672. © 2016 International Society for Magnetic Resonance in Medicine.

  17. Nearby Exo-Earth Astrometric Telescope (NEAT)

    Science.gov (United States)

    Shao, M.; Nemati, B.; Zhai, C.; Goullioud, R.

    2011-01-01

    NEAT (Nearby Exo ]Earths Astrometric Telescope) is a modest sized (1m diameter telescope) It will be capable of searching approx 100 nearby stars down to 1 Mearth planets in the habitable zone, and 200 @ 5 Mearth, 1AU. The concept addresses the major issues for ultra -precise astrometry: (1) Photon noise (0.5 deg dia field of view) (2) Optical errors (beam walk) with long focal length telescope (3) Focal plane errors , with laser metrology of the focal plane (4) PSF centroiding errors with measurement of the "True" PSF instead of using a "guess " of the true PSF, and correction for intra pixel QE non-uniformities. Technology "close" to complete. Focal plane geometry to 2e-5 pixels and centroiding to approx 4e -5 pixels.

  18. Neural network correction of astrometric chromaticity

    CERN Document Server

    Gai, M

    2005-01-01

    In this paper we deal with the problem of chromaticity, i.e. apparent position variation of stellar images with their spectral distribution, using neural networks to analyse and process astronomical images. The goal is to remove this relevant source of systematic error in the data reduction of high precision astrometric experiments, like Gaia. This task can be accomplished thanks to the capability of neural networks to solve a nonlinear approximation problem, i.e. to construct an hypersurface that approximates a given set of scattered data couples. Images are encoded associating each of them with conveniently chosen moments, evaluated along the y axis. The technique proposed, in the current framework, reduces the initial chromaticity of few milliarcseconds to values of few microarcseconds.

  19. Astrometric Discovery of GJ 164B

    Science.gov (United States)

    Pravdo, Steven H.; Shaklan, Stuart B.; Henry, Todd; Benedict, G. Fritz

    2004-01-01

    We discovered a low-mass companion to the M dwarf GJ 164 with the CCD-based imaging system of the Stellar Planet Survey astrometric program. The existence of GJ 164B was confirmed with Hubble Space Telescope NICMOS imaging observations. A high-dispersion spectral observation in V sets a lower limit of Deltam > 2.2 mag between the two components of the system. Based on our parallax value of 82 +/- 8 mas, we derive the following orbital parameters: P = 2.04 +/- 0.03 yr, a = 103 +/- 0.03, and M-total 0.265 +/- 0.020 M-circle dot. The component masses are M-A = 0.170 +/- 0.015 M-circle dot and M-B = 0.095 +/- 0/015 M-circle dot. Based on its mass, colors, and spectral properties, GJ 164B has spectral type M6-M8 V.

  20. A new probe of the small-scale primordial power spectrum: astrometric microlensing by ultracompact minihalos

    CERN Document Server

    Li, Fangda; Law, Nicholas M

    2012-01-01

    The dark matter enclosed in a density perturbation with a large initial amplitude (delta-rho/rho > 1e-3) collapses shortly after recombination and forms an ultracompact minihalo (UCMH). Their high central densities make UCMHs especially suitable for detection via astrometric microlensing: as the UCMH moves, it changes the apparent position of background stars. A UCMH with a mass larger than a few solar masses can produce a distinctive astrometric microlensing signal that is detectable by the space astrometry mission Gaia. If Gaia does not detect gravitational lensing by any UCMHs, then it establishes an upper limit on their abundance and constrains the amplitude of the primordial power spectrum for k~3500 Mpc^{-1}. These constraints complement the upper bound on the amplitude of the primordial power spectrum derived from limits on gamma-ray emission from UCMHs because the astrometric microlensing signal produced by an UCMH is maximized if the dark-matter annihilation rate is too low to affect the UCMH's densi...

  1. An Updated 2017 Astrometric Solution for Betelgeuse

    Science.gov (United States)

    Harper, G. M.; Brown, A.; Guinan, E. F.; O'Gorman, E.; Richards, A. M. S.; Kervella, P.; Decin, L.

    2017-07-01

    We provide an update for the astrometric solution for the Type II supernova progenitor Betelgeuse using the revised Hipparcos Intermediate Astrometric Data (HIAD) of van Leeuwen, combined with existing VLA and new e-MERLIN and ALMA positions. The 2007 Hipparcos refined abscissa measurements required the addition of so-called Cosmic Noise of 2.4 mas to find an acceptable 5-parameter stochastic solution. We find that a measure of radio Cosmic Noise should also be included for the radio positions because surface inhomogeneities exist at a level significant enough to introduce additional intensity centroid uncertainty. Combining the 2007 HIAD with the proper motions based solely on the radio positions leads to a parallax of π =5.27+/- 0.78 mas ({190}-25+33 pc), smaller than the Hipparcos 2007 value of 6.56 ± 0.83 mas ({152}-17+22 pc). Furthermore, combining the VLA and new e-MERLIN and ALMA radio positions with the 2007 HIAD, and including radio Cosmic Noise of 2.4 mas, leads to a nominal parallax solution of 4.51 ± 0.80 mas ({222}-34+48 pc), which, while only 0.7σ different from the 2008 solution of Harper et al., is 2.6σ different from the solution of van Leeuwen. An accurate and precise parallax for Betelgeuse is always going to be difficult to obtain because it is small compared to the stellar angular diameter (θ =44 mas). We outline an observing strategy utilizing future mm and sub-mm high-spatial resolution interferometry that must be used if substantial improvements in the precision and accuracy of the parallax and distance are to be achieved.

  2. Full-sky Astrometric Mapping Explorer (FAME)

    Science.gov (United States)

    Johnston, K.; Gaume, R.; Harris, F.; Monet, D.; Murison, M.; Seidelmann, P. K.; Urban, S.; Johnson, M.; Horner, S.; Vassar, R.

    2000-12-01

    The FAME project began Phase B development in September 2000. FAME is a MIDEX class NASA Explorer mission that will perform an all-sky, astrometric survey with unprecedented accuracy. FAME will produce an astrometric catalog of 40 million stars between 5th and 15th magnitude. For the bright stars (5th to 9th magnitude) FAME will determine positions and parallaxes accurate to better than 50 microarcseconds, with proper motion errors less than 50 microarcseconds per year. For the fainter stars (between 9th and 15th magnitude) FAME will determine positions and parallaxes accurate to better than 500 microarcseconds, with proper motion errors less than 500 microarcseconds per year. FAME will also collect photometric data on these 40 million stars in four Sloan DSS colors. The FAME science, instrument, and spacecraft requirements and error budgets are being refined to establish the basis for the improved design of the instrument and spacecraft. The Attitude Control System (ACS) based on solar radiation pressure is being studied, including the limitations on the solar angle between the Sun and the rotation angle. The data processing plans are being developed. The CCD procurement contract is in place and design and fabrication of the CCDs is in progress. CCD tests for operations in various Time Delay Integration (TDI) situations are underway and described in another poster. It appears that the current FAME launch schedule will be delayed somewhat due to recent NASA budget restrictions. The FAME project is funded by the NASA Explorer program administered by Goddard Space Flight Center for the Office of Space Science under contract number S-13610-Y.

  3. Transcriptome profiling reveals TGF-beta signaling involvement in epileptogenesis.

    Science.gov (United States)

    Cacheaux, Luisa P; Ivens, Sebastian; David, Yaron; Lakhter, Alexander J; Bar-Klein, Guy; Shapira, Michael; Heinemann, Uwe; Friedman, Alon; Kaufer, Daniela

    2009-07-15

    Brain injury may result in the development of epilepsy, one of the most common neurological disorders. We previously demonstrated that albumin is critical in the generation of epilepsy after blood-brain barrier (BBB) compromise. Here, we identify TGF-beta pathway activation as the underlying mechanism. We demonstrate that direct activation of the TGF-beta pathway by TGF-beta1 results in epileptiform activity similar to that after exposure to albumin. Coimmunoprecipitation revealed binding of albumin to TGF-beta receptor II, and Smad2 phosphorylation confirmed downstream activation of this pathway. Transcriptome profiling demonstrated similar expression patterns after BBB breakdown, albumin, and TGF-beta1 exposure, including modulation of genes associated with the TGF-beta pathway, early astrocytic activation, inflammation, and reduced inhibitory transmission. Importantly, TGF-beta pathway blockers suppressed most albumin-induced transcriptional changes and prevented the generation of epileptiform activity. Our present data identifies the TGF-beta pathway as a novel putative epileptogenic signaling cascade and therapeutic target for the prevention of injury-induced epilepsy.

  4. Transcriptome Profiling Reveals TGF-β Signaling Involvement in Epileptogenesis

    Science.gov (United States)

    Cacheaux, Luisa P; Ivens, Sebastian; David, Yaron; Lakhter, Alexander J; Bar-Klein, Guy; Shapira, Michael; Heinemann, Uwe; Friedman, Alon; Kaufer, Daniela

    2010-01-01

    Brain injury may result in the development of epilepsy, one of the most common neurological disorders. We previously demonstrated that albumin is critical in the generation of epilepsy following blood-brain barrier (BBB) compromise. Here we identify TGF-β pathway activation as the underlying mechanism. We demonstrate that direct activation of the TGF-β pathway by TGF-β1 results in epileptiform activity similar to that following exposure to albumin. Co-immunoprecipitation revealed binding of albumin to TGF-β receptor II and Smad2 phosphorylation confirmed downstream activation of this pathway. Transcriptome profiling demonstrated similar expression patterns following BBB breakdown, albumin and TGF-β1 exposure, including modulation of genes associated with the TGF-β pathway, early astrocytic activation, inflammation, and reduced inhibitory transmission. Importantly, TGF-β pathway blockers suppressed most albumin-induced transcriptional changes and prevented the generation of epileptiform activity. Our present data identifies the TGF-β pathway as a novel putative epileptogenic signaling cascade and therapeutic target for the prevention of injury-induced epilepsy. PMID:19605630

  5. Astrometric surveys: Solving the Milky Way puzzle with Gaia

    Directory of Open Access Journals (Sweden)

    Brown A.G.A.

    2012-02-01

    Full Text Available In this contribution I provide a brief summary of ongoing and future astrometric surveys and then focus on presenting the current status and expected scientific performance of the Gaia mission, scheduled for launch in 2013.

  6. A Search for Stellar-Mass Black Holes via Astrometric Microlensing

    CERN Document Server

    Lu, J R; Ofek, E O; Udalski, A; Kozlowski, S

    2016-01-01

    While dozens of stellar mass black holes have been discovered in binary systems, isolated black holes have eluded detection. Their presence can be inferred when they lens light from a background star. We attempt to detect the astrometric lensing signatures of three photometrically identified microlensing events, OGLE-2011-BLG-0022, OGLE-2011-BLG-0125, and OGLE-2012-BLG-0169 (OB110022, OB110125, and OB120169), located toward the Galactic Bulge. These events were selected because of their long durations, which statistically favors more massive lenses. Astrometric measurements were made over 1-2 years using laser-guided adaptive optics observations from the W. M. Keck Observatory. Lens model parameters were first constrained by the photometric light curves. The OB120169 light curve is well-fit by a single-lens model, while both OB110022 and OB110125 light curves favor binary-lens models. Using the photometric fits as prior information, no significant astrometric lensing signal was detected and all targets were c...

  7. Generating strain signals under consideration of road surface profiles

    Science.gov (United States)

    Putra, T. E.; Abdullah, S.; Schramm, D.; Nuawi, M. Z.; Bruckmann, T.

    2015-08-01

    The current study aimed to develop the mechanism for generating strain signal utilising computer-based simulation. The strain data, caused by the acceleration, were undertaken from a fatigue data acquisition involving car movements. Using a mathematical model, the measured strain signals yielded to acceleration data used to describe the bumpiness of road surfaces. The acceleration signals were considered as an external disturbance on generating strain signals. Based on this comparison, both the actual and simulated strain data have similar pattern. The results are expected to provide new knowledge to generate a strain signal via a simulation.

  8. Astrometric Calibration of the Gemini Planet Imager

    Science.gov (United States)

    Tran, Debby; Konopacky, Quinn M.; GPIES Team

    2017-01-01

    The Gemini Planet Imager (GPI), housed on the 8-meter Gemini South telescope in Chile, is an instrument designed to detect Jupiter-like extrasolar planets by direct imaging. It relies on adaptive optics to correct the effects of atmospheric turbulence, along with an advanced coronagraph and calibration system. One of the scientific goals of GPI is to measure the orbital properties of the planets it discovers. Because these orbits have long periods, precise measurements of the relative position between the star and the planet (relative astrometry) are required. In this poster, I will present the astrometric calibration of GPI. We constrain the plate scale and orientation of the camera by observing different binary star systems with both GPI and another well-calibrated instrument, NIRC2, at the Keck telescope in Hawaii. We measure their separations with both instruments and use that information to calibrate the plate scale. By taking these calibration measurements over the course of one year, we have measured the plate scale to 0.05% and shown that it is stable across multiple epochs. We also examined the effects of the point spread function on the positions of the binaries as well as their separations, the results of which I will discuss.

  9. The Laser Astrometric Test of Relativity Mission

    Science.gov (United States)

    Turyshev, Slava G.; Shao, Michael; Nordtvedt, Kenneth L.

    2004-09-01

    This paper discusses new fundamental physics experiment to test relativistic gravity at the accuracy better than the effects of the 2nd order in the gravitational field strength, ∝ G2. The Laser Astrometric Test Of Relativity (LATOR) mission uses laser interferometry between two micro-spacecraft whose lines of sight pass close by the Sun to accurately measure deflection of light in the solar gravity. The key element of the experimental design is a redundant geometry optical truss provided by a long-baseline (100 m) multi-channel stellar optical interferometer placed on the International Space Station (ISS). The interferometer is used for measuring the angles between the two spacecraft. In Euclidean geometry, determination of a triangle's three sides determines any angle therein; with gravity changing the optical lengths of sides passing close by the Sun and deflecting the light, the Euclidean relationships are overthrown. The geometric redundancy enables LATOR to measure the departure from Euclidean geometry caused by the solar gravity field to a very high accuracy. LATOR will not only improve the value of the parameterized post-Newtonian (PPN) parameter γ to unprecedented levels of accuracy of 10-8, it will also reach ability to measure effects of the next post-Newtonian order (c-4) of light deflection resulting from gravity's intrinsic non-linearity. The solar quadrupole moment parameter, J2, will be measured with high precision, as well as a variety of other relativistic effects including Lense-Thirring precession. LATOR will lead to very robust advances in the tests of fundamental physics: this mission could discover a violation or extension of general relativity, or reveal the presence of an additional long range interaction in the physical law. There are no analogs to the LATOR experiment; it is unique and is a natural culmination of solar system gravity experiments.

  10. Enhanced Phosphoproteomic Profiling Workflow For Growth Factor Signaling Analysis

    DEFF Research Database (Denmark)

    Sylvester, Marc; Burbridge, Mike; Leclerc, Gregory;

    2010-01-01

    Background Our understanding of complex signaling networks is still fragmentary. Isolated processes have been studied extensively but cross-talk is omnipresent and precludes intuitive predictions of signaling outcomes. The need for quantitative data on dynamic systems is apparent especially for our...... A549 lung carcinoma cells were used as a model and stimulated with hepatocyte growth factor, epidermal growth factor or fibroblast growth factor. We employed a quick protein digestion workflow with spin filters without using urea. Phosphopeptides in general were enriched by sequential elution from...... transfer dissociation adds confidence in modification site assignment. The workflow is relatively simple but the integration of complementary techniques leads to a deeper insight into cellular signaling networks and the potential pharmacological intervention thereof....

  11. Signal Temporal Profile of a Water Cherenkov Detector

    Science.gov (United States)

    Salazar, H.; Martinez, O.; Cotzomi, J.; Moreno, E.; Villaseñor, L.

    2003-07-01

    The suggested existence of temporal structure in the signals of extensive air showers (EAS) for energies greater than 1017 eV at core distances of about 500 m, and its correlation with important parameters of EASs has stimulated us to study this structure for showers with lower energies in an Auger water Cherenkov detector(WCD). Preliminary analysis of experimental data on the widths of signals in a WCD and their correlation with other parameters of the signal are presented. The detector was triggered by the EAS-BUAP array which operates in the region of 1014 - 1016 eV. The distance of the WCD to the EAS core is larger than 30 m.

  12. Analogue Simulation and Orbital Solving Algorithm of Astrometric Exoplanet Detection

    Science.gov (United States)

    Huang, P. H.; Ji, J. H.

    2016-09-01

    Astrometry is an effective method to detect exoplanets. It has many advantages that other detection methods do not bear, such as providing three dimensional planetary orbit and determining the planetary mass. Astrometry will enrich the sample of exoplanets. As the high-precision astrometric satellite Gaia (Global Astrometry interferometer for Astrophysics) was launched in 2013, there will be abundant long-period Jupiter-size planets to be discovered by Gaia. In this paper, we specify the α Centauri A, HD 62509, and GJ 876 systems, and generate the synthetic astrometric data with the single astrometric precision of Gaia. Then we use the Lomb-Scargle periodogram to analyse the signature of planets and the Markov Chain Monte Carlo (MCMC) algorithm to fit the orbit of planets. The simulation results are well coincide with the initial solutions.

  13. The Full-sky Astrometric Mapping Explorer - An optical, astrometric survey mission

    Science.gov (United States)

    Horner, S. D.; Germain, M. E.; Greene, T. P.; Harris, F. H.; Harris, H. C.; Johnson, M. S.; Johnston, K. J.; Monet, D. G.; Murison, M. A.; Phillips, J. D.; Reasenberg, R. D.; Seidelmann, P. K.; Urban, S. E.; Vassar, R. H.

    1999-12-01

    The Full-sky Astrometric Mapping Explorer (FAME) is a NASA MIDEX mission scheduled for launch in 2004. It will perform an all sky, astrometric survey with unprecedented accuracy. FAME will determine the positions, parallaxes, proper motions, and photometry of 40,000,000 stars with visual band magnitudes 5 DSS colors. FAME will enable a wide range of scientific investigations using its large, rich database of information on stellar properties. It will: * Calibrate the zero point of the extragalactic distance scale to 1% * Determine absolute luminosities of a wide range of spectral types * Detect a meaningful statistical sample of companion stars, brown dwarfs, and giant planets * Enable studies of the kinematics of our galaxy, including the effect of dark matter in the disk * Characterize stellar variability of a large sample of stars at the 0.1% level * Define a rigid optical reference frame for future scientific endeavors FAME is evolved from design concepts from the Hipparcos mission, using current CCD technology to observe more and fainter stars. Like Hipparcos, FAME has a compound mirror consisting of two flats angled relative to each other. The compound mirror feeds the two fields of view separated by the ``basic angle'' into a common telescope. The two fields of view are used to control the growth of stochastic errors in determining the relative separations of stars. FAME is a joint development effort of the U.S. Naval Observatory, the Naval Research Laboratory, Lockheed Martin Missiles and Space Advanced Technology Center, and the Smithsonian Astrophysical Observatory. Funding for FAME is provided by the NASA Office of Space Science through the Explorer program managed by Goddard Space Flight Center. Additional funding has been provided by the U.S. Navy. http://www.usno.navy.mil/fame

  14. The laser astrometric test of relativity mission

    Energy Technology Data Exchange (ETDEWEB)

    Turyshev, Slava G [Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Drive, Pasadena, CA 91109 (United States); Shao, Michael [Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Drive, Pasadena, CA 91109 (United States); Nordtvedt, Kenneth [Northwest Analysis, 118 Sourdough Ridge Road, Bozeman, MT 59715 (United States)

    2004-06-21

    This paper discusses the motivation and general design elements of a new fundamental physics experiment that will test relativistic gravity at the accuracy better than the effects of the second order in the gravitational field strength, {proportional_to}G{sup 2}. The laser astrometric test of relativity (LATOR) mission uses laser interferometry between two micro-spacecraft whose lines of sight pass close by the Sun to accurately measure deflection of light in the solar gravity. The key element of the experimental design is a redundant geometry optical truss provided by a long-baseline (100 m) multi-channel stellar optical interferometer placed on the International Space Station (ISS). The spatial interferometer is used for measuring the angles between the two spacecraft and for orbit determination purposes. In Euclidean geometry, determination of a triangle's three sides determines any angle therein; with gravity changing the optical lengths of sides passing close by the Sun and deflecting the light, the Euclidean relationships are overthrown. The geometric redundancy enables LATOR to measure the departure from Euclidean geometry caused by the solar gravity field to a very high accuracy. LATOR will not only improve the value of the parametrized post-Newtonian (PPN) parameter {gamma} to unprecedented levels of accuracy of 1 part in 10{sup 8}, it will also reach the ability to measure effects of the next post-Newtonian order ({proportional_to}G{sup 2}) of light deflection resulting from gravity's intrinsic nonlinearity. The solar quadrupole moment parameter, J{sub 2}, will be measured with high precision, as well as a variety of other relativistic effects including Lense-Thirring precession. LATOR will lead to very robust advances in the tests of fundamental physics: this mission could discover a violation or extension of general relativity, or reveal the presence of an additional long range interaction in the physical law. There are no analogues to the

  15. Profile and Instrumentation Driven Methods for Embedded Signal Processing

    Science.gov (United States)

    2015-01-01

    and verification [35]. Another example is the Architecture Analysis & Design Language (AADL) which has been used in automotive and avionics fields to...interference in multitasked embedded workloads , but also further lever- ages compiler-generated application information to preload TLB entries that will be...multitasked workloads . CTP requires profiling the application to identify hotspots, which can be done manually using a tool such as gprof or achieved by using

  16. Forthcoming mutual events of planets and astrometric radio sources

    CERN Document Server

    Malkin, Z; Tsekmejster, S

    2013-01-01

    Radio astronomy observations of close approaches of the Solar system planets to compact radio sources as well as radio source occultations by planets may be of large interest for planetary sciences, dynamical astronomy, and testing gravity theories. In this paper, we present extended lists of occultations of astrometric radio sources observed in the framework of various astrometric and geodetic VLBI programs by planets, and close approaches of planets to radio sources expected in the nearest years. Computations are made making use of the EPOS software package.

  17. Enabling interferometry technologies for the GAIA astrometric mission

    Science.gov (United States)

    Bisi, M.; Bonino, L.; Cecconi, Massimo; Cesare, Stefano; Bertinetto, Fabrizio; Mana, Giovanni; Carollo, D.; Gai, Mario; Lattanzi, Mario G.; Canuto, Enrico; Donati, F.

    1999-08-01

    Within a Technology Research Program funded by the European Space Agency, a team led by Alenia Aerospazio has investigated and started the development of some technologies which are considered fundamental for the achievement of the scientific objectives of the future astrometric mission GAIA. The activities have been focused on the design of a two-aperture optical interferometer and of a system for the active stabilization of its configuration within few picometers. A laboratory prototype of the active stabilization system has been implemented and tested. The results achieved in the laboratory tests proved that the very challenging requirements imposed by the GAIA astrometric goal of 10 micro-arcsec accuracy can be fulfilled.

  18. Forthcoming Occultations of Astrometric Radio Sources by Planets

    Science.gov (United States)

    L'vov, Victor; Malkin, Zinovy; Tsekmeister, Svetlana

    2010-01-01

    Astrometric observations of radio source occultations by solar system bodies may be of large interest for testing gravity theories, dynamical astronomy, and planetary physics. In this paper, we present an updated list of the occultations of astrometric radio sources by planets expected in the coming years. Such events, like solar eclipses, generally speaking can only be observed in a limited region. A map of the shadow path is provided for the events that will occurr in regions with several VLBI stations and hence will be the most interesting for radio astronomy experiments.

  19. Gamma Astrometric Measurement Experiment (GAME) - Science case

    Science.gov (United States)

    Vecchiato, Alberto; Gai, Mario; Lattanzi, Mario G.; Crosta, Maria Teresa; Sozzetti, Alessandro

    GAME (Gamma Astrometric Measurement Experiment) is a concept for a small mission whose main goal is to measure from space the γ parameter of the Parameterized Post-Newtonian formalism. A satellite looking as close as possible to the Solar limb implements a technique similar to that used during the solar eclipse of 1919, when Dyson, Eddington and collaborators measured for the first time the gravitational bending of light. Preliminary simulations have shown that the expected final accuracy can reach the 10-7 level or better. This makes GAME a decisive experiment for the understanding of gravity physics, cosmology and the Universe evolution at a fundamental level. During the last decade, in fact, a strong experimental evidence of an acceleration of the expansion of the Universe at the present time has been provided by several observational data. This has been interpreted as the effect of a long range perturbation of the gravity field of the visible matter generated by the so-called Dark Energy. These data add to those available for long time at different scale length, which are explained with the existence of non-barionic Dark Matter (e.g. galaxy rotation curves) or with some kind of modification of the General Relativity theory (e.g. Pioneer anomalies). However, there are claims that these data can be explained with a modified version of General Relativity, in which the curvature invariant R is no longer constant in the Einstein equations (f (R) gravity theories). Present experimental data are not accurate enough to discriminate between these scenarios, but this could be done with a 10-7 -level measure of γ. Moreover, the limited fraction of time needed for the main experiment with respect to the overall mission duration opens interesting possibilities for other kinds of measurements. One is to measure the light deflection induced by the quadrupole moment of giant planets like Jupiter or Saturn, an effect predicted by General Relativity but never measured up to

  20. Optical design for the Laser Astrometric Test of Relativity

    Science.gov (United States)

    Turyshev, Slava G.; Shao, Michael; Nordtvedt, Kenneth L., Jr.

    2004-01-01

    This paper discusses the Laser Astrometric Test of Relativity (LATOR) mission. LATOR is a Michelson-Morley-type experiment designed to test the pure tensor metric nature of gravitation the fundamental postulate of Einstein's theory of general relativity. With its focus on gravity's action on light propagation it complements other tests which rely on the gravitational dynamics of bodies.

  1. A new method for astrometric observations of asteroids

    Science.gov (United States)

    Pauwels, Thierry

    1992-01-01

    In this paper we propose a new method for photographic astrometric observations of asteroids. We discuss its advantages and disadvantages and compare them to the advantages and disadvantages of the classical photographic methods. The new method is best suited for observations on a spot where no CCD cameras, blink or stereo comparators are available and when a fast detection of unknown objects is required.

  2. Loss of embryonic MET signaling alters profiles of hippocampal interneurons.

    Science.gov (United States)

    Martins, Gabriela J; Plachez, Céline; Powell, Elizabeth M

    2007-01-01

    Hippocampal interneurons arise in the ventral forebrain and migrate dorsally in response to cues, including hepatocyte growth factor/scatter factor which signals via its receptor MET. Examination of the hippocampus in adult mice in which MET had been inactivated in the embryonic proliferative zones showed an increase in parvalbumin-expressing cells in the dentate gyrus, but a loss of these cells in the CA3 region. An overall loss of calretinin-expressing cells was seen throughout the hippocampus. A similar CA3 deficit of parvalbumin and calretinin cells was observed when MET was eliminated only in postmitotic cells. These data suggest that MET is required for the proper hippocampal development, and embryonic perturbations lead to long-term anatomical defects with possible learning and memory dysfunction.

  3. HIGH RESOLUTION RANGE PROFILE FORMATION BASED ON LFM SIGNAL FUSION OF MULTIPLE RADARS

    Institute of Scientific and Technical Information of China (English)

    Wang Cheng; Hu Weidong; Du Xiaoyong; Yu Wenxian

    2007-01-01

    This paper presents a new method of High Resolution Range (HRR) profile formation based on Linear Frequency Modulation (LFM) signal fusion of multiple radars with multiple frequency bands.The principle of the multiple radars signal fusion improving the range resolution is analyzed. With the analysis of return signals received by two radars, it is derived that the phase difference between the echoes varies almost linearly with respect to the frequency if the distance between two radars is negligible compared with the radar observation distance. To compensate the phase difference, an entropy-minimization principle based compensation algorithm is proposed. During the fusion process, the B-splines interpolation method is applied to resample the signals for Fourier transform imaging. The theoretical analysis and simulations results show the proposed method can effectively increase signal bandwidth and provide a high resolution range profile.

  4. Signal transduction pathway profiling of individual tumor samples

    Directory of Open Access Journals (Sweden)

    Peterson Carsten

    2005-06-01

    Full Text Available Abstract Background Signal transduction pathways convey information from the outside of the cell to transcription factors, which in turn regulate gene expression. Our objective is to analyze tumor gene expression data from microarrays in the context of such pathways. Results We use pathways compiled from the TRANSPATH/TRANSFAC databases and the literature, and three publicly available cancer microarray data sets. Variation in pathway activity, across the samples, is gauged by the degree of correlation between downstream targets of a pathway. Two correlation scores are applied; one considers all pairs of downstream targets, and the other considers only pairs without common transcription factors. Several pathways are found to be differentially active in the data sets using these scores. Moreover, we devise a score for pathway activity in individual samples, based on the average expression value of the downstream targets. Statistical significance is assigned to the scores using permutation of genes as null model. Hence, for individual samples, the status of a pathway is given as a sign, + or -, and a p-value. This approach defines a projection of high-dimensional gene expression data onto low-dimensional pathway activity scores. For each dataset and many pathways we find a much larger number of significant samples than expected by chance. Finally, we find that several sample-wise pathway activities are significantly associated with clinical classifications of the samples. Conclusion This study shows that it is feasible to infer signal transduction pathway activity, in individual samples, from gene expression data. Furthermore, these pathway activities are biologically relevant in the three cancer data sets.

  5. Astrometric and Photometric Accuracy of the 1.3 m Robotically Controlled Telescope on Kitt Peak

    Science.gov (United States)

    McGruder, Charles H.; Carini, M. T.; Engle, S. G.; Gelderman, R.; Guinan, E. F.; Laney, D.; Strolger, L.; Treffers, R. R.; Walter, D. K.

    2014-01-01

    The 1.3 m (50 inch) telescope on Kitt Peak has been refurbished and provided with an autonomous scheduler. It is operated by The Robotically Controlled Telescope (RCT) consortium whose members are: South Carolina State, Villanova and Western Kentucky Universities. The facility possesses 5 board (UBVRI) and 11 narrow-band filters. Attached to the RCT camera is a 2048 x 2048 SITe SI-424A back-illuminated CCD with 24 micrometer pixels. We used over 7,000 star measurements from 37, 198s R-images to compute the astrometric and photometric accuracy. The difference of the J2000 coordinates computed from the RCT images and the J2000 Nomad catalog coordinate values in right ascension peaks at 0.058”, while the declination peaks at -0.125”. We obtained these astrometric results using the simplest assumptions: linear relationship between standard coordinates and measured coordinates, no color or magnitude dependency and no differential refraction (all images taken in the zenith). We express the photometric accuracy in the following manner: The Signal-to-Noise-Ratio as a function of apparent magnitude shows that the RCT is not noise dominated at m < 20 magnitude.

  6. Utilizing Astrometric Orbits to Obtain Coronagraphic Images of Extrasolar Planets

    Science.gov (United States)

    Davidson, John M.

    2011-08-01

    We present an approach for utilizing astrometric orbit information to improve the yield of planetary images and spectra from a follow-on direct-detection mission. This approach is based on the notion—strictly hypothetical—that if a particular star could be observed continuously, the instrument would in time observe all portions of the habitable zone so that no planet residing therein could be missed. This strategy could not be implemented in any realistic mission scenario. But if an exoplanet’s orbit is known from astrometric observation, then it may be possible to plan and schedule a sequence of imaging observations that is the equivalent of continuous observation. A series of images—optimally spaced in time—could be recorded to examine contiguous segments of the orbit. In time, all segments would be examined, leading to the inevitable detection of the planet. In this article, we show how astrometric orbit information can be used to construct such a sequence. We apply this methodology to seven stars taken from the target lists of proposed astrometric and direct-detection missions. In addition, we construct this sequence for the Sun-Earth system as it would appear from a distance of 10 pc. In constructing these sequences, we have assumed that the imaging instrument has an inner working angle (IWA) of 75 mas and that the planets are visible whenever they are separated from their host stars by ≥IWA and are in quarter-phase or greater. In addition, we have assumed that the planets orbit at a distance of 1 AU scaled to luminosity and that the inclination of the orbit plane is 60°. For the individual stars in this target pool, we find that the number of observations in this sequence ranges from two to seven, representing the maximum number of observations required to find the planet. The probable number of observations ranges from 1.5 to 3.1. These results suggest that a direct-detection mission using astrometric orbits would find all eight exoplanets in

  7. Finding Free-Floating Black Holes using Astrometric Microlensing

    Science.gov (United States)

    Lu, Jessica R.; Ofek, Eran Oded; Sinukoff, Evan; Udalski, Andrzej; Kozlowski, Szymon

    2017-01-01

    Our Galaxy most likely hosts 10-100 million stellar mass black holes. The exact number and mass function of these black holes contains important information regarding our Galaxy's star formation history, stellar mass function, and the fate of very massive stars. However, isolated stellar black holes have yet to be detected. To date, stellar mass black holes have only been definitively detected in binary systems with accreting companions or merging to produce gravitational waves. In principle, the presence of isolated black holes can be inferred from astrometric and photometric signatures produced when they lens light from a background star. We attempt to detect the astrometric lensing signatures of several photometrically identified microlensing events, toward the Galactic Bulge. Long-duration events (t_Einstein > 100 days) were selected as the most likely black hole candidates and were observed using several years of laser-guided adaptive optics observations from the W. M. Keck telescopes. We present results from this search.

  8. Resolved astrometric orbits of ten O-type binaries

    CERN Document Server

    Bouquin, J -B Le; Gosset, E; De Becker, M; Duvert, G; Absil, O; Anthonioz, F; Berger, J -P; Ertel, S; Grellmann, R; Guieu, S; Kervella, P; Rabus, M; Willson, M

    2016-01-01

    Our long term aim is to derive model-independent stellar masses and distances for long period massive binaries by combining apparent astrometric orbit with double-lined radial velocity amplitudes (SB2). We follow-up ten O+O binaries with AMBER, PIONIER and GRAVITY at the VLTI. Here, we report about 130 astrometric observations over the last 7 years. We combine this dataset with distance estimates to compute the total mass of the systems. We also compute preliminary individual component masses for the five systems with available SB2 radial velocities. Nine over the ten binaries have their three dimensional orbit well constrained. Four of them are known colliding wind, non-thermal radio emitters, and thus constitute valuable targets for future high angular resolution radio imaging. Two binaries break the correlation between period and eccentricity tentatively observed in previous studies. It suggests either that massive star formation produce a wide range of systems, or that several binary formation mechanisms ...

  9. Astrometric Gravitation Probe: a space mission concept for fundamental physics

    Science.gov (United States)

    Vecchiato, Alberto; Fienga, Agnes; Gai, Mario; Lattanzi, Mario G.; Riva, Alberto; Busonero, Deborah

    2015-08-01

    Modern technological developments have pushed the accuracy of astrometric measurements in the visible band down to the micro-arcsec level. This allows to test theories of gravity in the weak field limit to unprecedented level, with possible consequences spanning from the validity of fundamental physics principles, to tests of theories describing cosmological and galactic dynamics without resorting to Dark Matter and Dark Energy.This is the main goal of Astrometric Gravitation Probe (AGP) mission, which will be achieved by highly accurate astrometric determination of light deflection (as a modern rendition of the Dyson, Eddington, and Robertson eclipse experiment of 1919), aberration, and of the orbits of selected Solar System objects, with specific reference to the excess shift of the pericentre effect.The AGP concept was recently proposed for the recent call for ESA M4 missions as a collaboration among several scientists coming from many different European and US institutions. Its payload is based on a 1.15 m diameter telescope fed through a coronagraphic system by four fields, two set in symmetric positions around the Sun, and two in the opposite direction, all imaged on a CCD detector. Large parts of the instrument are common mode to all fields. The baseline operation mode is the scan of the ±1.13 deg Ecliptic strip, repeated for a minimum of 3 years and up to an optimal duration of 5 years. Operations and calibrations are simultaneous, defined in order to ensure common mode instrumental effects, identified and removed in data reduction. The astrometric and coronagraphic technologies build on the heritage of Gaia and Solar Orbiter.We review the mission concept and its science case, and discuss how this measurement concepts can be scaled to different mission implementations.

  10. Astrometric Calibration and Performance of the Dark Energy Camera

    Energy Technology Data Exchange (ETDEWEB)

    Bernstein, G.M.; et al.

    2017-03-05

    We characterize the ability of the Dark Energy Camera (DECam) to perform relative astrometry across its 500 Mpix, 3 $deg^2$ science field of view, and across 4 years of operation. This is done using internal comparisons of $~ 4 x 10^7$ measurements of high-S/N stellar images obtained in repeat visits to fields of moderate stellar density, with the telescope dithered to move the sources around the array. An empirical astrometric model includes terms for: optical distortions; stray electric fields in the CCD detectors; chromatic terms in the instrumental and atmospheric optics; shifts in CCD relative positions of up to $\\approx 10 \\mu m$ when the DECam temperature cycles; and low-order distortions to each exposure from changes in atmospheric refraction and telescope alignment. Errors in this astrometric model are dominated by stochastic variations with typical amplitudes of 10-30 mas (in a 30 s exposure) and $5^{\\prime}-10^{\\prime}$ arcmin coherence length, plausibly attributed to Kolmogorov-spectrum atmospheric turbulence. The size of these atmospheric distortions is not closely related to the seeing. Given an astrometric reference catalog at density $\\approx 0.7$ $arcmin^{-2}$, e.g. from Gaia, the typical atmospheric distortions can be interpolated to $\\approx$ 7 mas RMS accuracy (for 30 s exposures) with $1^{\\prime}$ arcmin coherence length for residual errors. Remaining detectable error contributors are 2-4 mas RMS from unmodelled stray electric fields in the devices, and another 2-4 mas RMS from focal plane shifts between camera thermal cycles. Thus the astrometric solution for a single DECam exposure is accurate to 3-6 mas ( $\\approx$ 0.02 pixels, or $\\approx$ 300 nm) on the focal plane, plus the stochastic atmospheric distortion.

  11. The Full-sky Astrometric Mapping Explorer Concept Study

    Science.gov (United States)

    Horner, S. D.; Germain, M. E.; Greene, T. P.; Harris, F. H.; Johnson, M. S.; Johnston, K. J.; Monet, D. G.; Murison, M. A.; Phillips, J. D.; Reasenberg, R. D.; Seidelmann, P. K.; Talabac, S. J.; Urban, S. E.; van Buren, D.; Vassar, R. H.

    1999-05-01

    NASA has selected the Full-sky Astrometric Mapping Explorer (FAME) to be one of five MIDEX missions to be funded for a concept study. This concept study will be submitted to NASA on 18 June, with final selection, scheduled for September, of two of these missions for flight in 2003 or 2004. FAME is designed to perform an all-sky, astrometric survey with unprecedented accuracy. It will create a rigid astrometric catalog of 40,000,000 stars with visual band magnitudes 5 DSS colors. During the concept study, the team has worked to optimize the scientific return from FAME while minimizing cost and risk. The optical design was modified for improved accuracy of individual observations and improved mechanical design. The optical, mechanical, and thermal design of the instrument have been improved. Tests using CCDs in TDI mode are being conducted to confirm the accuracy obtainable from individual observations as well as determine the optimal clocking scheme for astrometric devices operated in TDI mode. The use of solar radiation pressure for spacecraft precession has undergone further feasibility study, as have the mechanisms for deploying the solar shield. Numerous other trade studies have been conducted, including orbit/communications, on board processing, and the use of neutral density filters for astrometry of bright stars versus other options. A detailed error budget has been formulated and the mission requirements have been defined. We look forward to selection for launch and a successful FAME mission that will redefine the extragalactic distance scale and provide a large, rich database of information on stellar properties that will enable numerous science investigations into stellar structure and evolution, the dynamics of the Milky Way, and stellar companions including brown dwarfs and giant planets. FAME is a joint development effort of the US Naval Observatory, the Smithsonian Astrophysical Observatory, the Infrared Processing and Analysis Center, Lockheed Martin

  12. Method for measuring radial impurity emission profiles using correlations of line integrated signals

    Science.gov (United States)

    Kuldkepp, M.; Brunsell, P. R.; Drake, J.; Menmuir, S.; Rachlew, E.

    2006-04-01

    A method of determining radial impurity emission profiles is outlined. The method uses correlations between line integrated signals and is based on the assumption of cylindrically symmetric fluctuations. Measurements at the reversed field pinch EXTRAP T2R show that emission from impurities expected to be close to the edge is clearly different in raw as well as analyzed data to impurities expected to be more central. Best fitting of experimental data to simulated correlation coefficients yields emission profiles that are remarkably close to emission profiles determined using more conventional techniques. The radial extension of the fluctuations is small enough for the method to be used and bandpass filtered signals indicate that fluctuations below 10kHz are cylindrically symmetric. The novel method is not sensitive to vessel window attenuation or wall reflections and can therefore complement the standard methods in the impurity emission reconstruction procedure.

  13. Astrometric precision of observations at VLT/FORS2

    CERN Document Server

    Lazorenko, P F

    2005-01-01

    In this paper we test the astrometric precision of VLT/FORS2 observations using a serie of CCD frames taken in Galactic bulge area. A special reduction method based on symmetrization of reference fields was used to reduce the atmospheric image motion. Positional precision of unsaturated R=16 mag star images at 17 sec exposure and 0.55 arcsec seeing was found to be equal to 300 microarcsec. The total error of observations was decomposed into components. It was shown that astrometric error depends largely on the photon centroiding error of the target (250 microarcsec for 16 mag stars) while the image motion is much less (110 microarcsec). At galactic latitudes to about 20 degrees, precision for a serie of frames with a 10 min total exposure is estimated to be 30-50 microarcsec for 14-16 mag stars providing the images are not overexposed and the filter R "special" is used. Error estimates for fields with smaller sky star density are given. We conclude that astrometric observations with large telescopes, under op...

  14. Stray light evaluation for the astrometric gravitation probe mission

    Science.gov (United States)

    Landini, Federico; Riva, Alberto; Gai, Mario; Baccani, Cristian; Focardi, Mauro; Pancrazzi, Maurizio

    2016-08-01

    The main goal of the Astrometric Gravitation Probe mission is the verification of General Relativity and competing gravitation theories by precise astrometric determination of light deflection, and of orbital parameters of selected Solar System objects. The key element is the coherent combination of a set of 92 circular entrance apertures, each feeding an elementary inverted occulter similar to the one developed for Solar Orbiter/METIS.1 This provides coronagraphic functions over a relevant field of view, in which all stars are observed for astrometric purposes with the full resolution of a 1 m diameter telescope. The telescope primary mirror acts as a beam combiner, feeding the 92 pupils, through the internal optics, toward a single focal plane. The primary mirror is characterized by 92 output apertures, sized according to the entrance pupil and telescope geometry, in order to dump the solar disk light beyond the instrument. The astronomical objects are much fainter than the solar disk, which is angularly close to the inner field of view of the telescope. The stray light as generated by the diffraction of the solar disk at the edges of the 92 apertures defines the limiting magnitude of observable stars. In particular, the stray light due to the diffraction from the pupil apertures is scattered by the telescope optics and follows the same optical path of the astronomical objects; it is a contribution that cannot be eliminated and must therefore be carefully evaluated. This paper describes the preliminary evaluation of this stray light contribution.

  15. Probabilistic Inference on Multiple Normalized Signal Profiles from Next Generation Sequencing: Transcription Factor Binding Sites

    KAUST Repository

    Wong, Ka-Chun

    2015-04-20

    With the prevalence of chromatin immunoprecipitation (ChIP) with sequencing (ChIP-Seq) technology, massive ChIP-Seq data has been accumulated. The ChIP-Seq technology measures the genome-wide occupancy of DNA-binding proteins in vivo. It is well-known that different DNA-binding protein occupancies may result in a gene being regulated in different conditions (e.g. different cell types). To fully understand a gene\\'s function, it is essential to develop probabilistic models on multiple ChIP-Seq profiles for deciphering the gene transcription causalities. In this work, we propose and describe two probabilistic models. Assuming the conditional independence of different DNA-binding proteins\\' occupancies, the first method (SignalRanker) is developed as an intuitive method for ChIP-Seq genome-wide signal profile inference. Unfortunately, such an assumption may not always hold in some gene regulation cases. Thus, we propose and describe another method (FullSignalRanker) which does not make the conditional independence assumption. The proposed methods are compared with other existing methods on ENCODE ChIP-Seq datasets, demonstrating its regression and classification ability. The results suggest that FullSignalRanker is the best-performing method for recovering the signal ranks on the promoter and enhancer regions. In addition, FullSignalRanker is also the best-performing method for peak sequence classification. We envision that SignalRanker and FullSignalRanker will become important in the era of next generation sequencing. FullSignalRanker program is available on the following website: http://www.cs.toronto.edu/∼wkc/FullSignalRanker/ © 2015 IEEE.

  16. Expression profiling and functional analysis of wnt signaling mechanisms in mesenchymal stem cells.

    Science.gov (United States)

    Etheridge, S Leah; Spencer, Gary J; Heath, Deborah J; Genever, Paul G

    2004-01-01

    Through their broad differentiation potential, mesenchymal stem cells (MSCs) are candidates for a range of therapeutic applications, but the precise signaling pathways that determine their differentiated fate are not fully understood. Evidence is emerging that developmental signaling cues may be important in regulating stem cell self-renewal and differentiation programs. Here we have identified a consistent expression profile of Wnt signaling molecules in MSCs and provide evidence that an endogenous canonical Wnt pathway functions in these cells. Wnts bind to Frizzled (Fz) receptors and subsequent canonical signaling inhibits glycogen synthase kinase-3beta (GSK-3beta), causing beta-catenin translocation into the nucleus to induce target gene expression. In human MSCs isolated from bone marrow of different donors, we appear to have identified a common Wnt/Fz expression profile using reverse transcriptase polymerase chain reaction (RT-PCR). Associated Wnt signaling components, including low-density lipoprotein receptor-related protein-5 (LRP-5), kremen-1, dickkopf-1 (Dkk-1), secreted Frizzled-related peptide (sFRP)-2, sFRP3, sFRP4, Disheveled (Dvl), GSK-3beta, adenomatous polyposis coli (APC), beta-catenin,T-cell factor (TCF)-1, and TCF-4, were also identified. Nuclear beta-catenin was observed in 30%-40% of MSCs, indicative of endogenous Wnt signaling. Exposure to both Wnt3a and Li+ ions, which promotes canonical Wnt signaling by inhibiting GSK-3beta, reduced phosphorylation of beta-catenin in MSCs and increased beta-catenin nuclear translocation approximately threefold over that of the controls. Our findings indicate that autocrine Wnt signaling operates in primitive MSC populations and supports previous evidence that Wnt signaling regulates mesenchymal lineage specification. The identification of a putative common Wnt/Fz molecular signature in MSCs will contribute to our understanding of the molecular mechanisms that regulate self-renewal and lineage

  17. Inverse methods for estimating primary input signals from time-averaged isotope profiles

    Science.gov (United States)

    Passey, Benjamin H.; Cerling, Thure E.; Schuster, Gerard T.; Robinson, Todd F.; Roeder, Beverly L.; Krueger, Stephen K.

    2005-08-01

    Mammalian teeth are invaluable archives of ancient seasonality because they record along their growth axes an isotopic record of temporal change in environment, plant diet, and animal behavior. A major problem with the intra-tooth method is that intra-tooth isotope profiles can be extremely time-averaged compared to the actual pattern of isotopic variation experienced by the animal during tooth formation. This time-averaging is a result of the temporal and spatial characteristics of amelogenesis (tooth enamel formation), and also results from laboratory sampling. This paper develops and evaluates an inverse method for reconstructing original input signals from time-averaged intra-tooth isotope profiles. The method requires that the temporal and spatial patterns of amelogenesis are known for the specific tooth and uses a minimum length solution of the linear system Am = d, where d is the measured isotopic profile, A is a matrix describing temporal and spatial averaging during amelogenesis and sampling, and m is the input vector that is sought. Accuracy is dependent on several factors, including the total measurement error and the isotopic structure of the measured profile. The method is shown to accurately reconstruct known input signals for synthetic tooth enamel profiles and the known input signal for a rabbit that underwent controlled dietary changes. Application to carbon isotope profiles of modern hippopotamus canines reveals detailed dietary histories that are not apparent from the measured data alone. Inverse methods show promise as an effective means of dealing with the time-averaging problem in studies of intra-tooth isotopic variation.

  18. Comparative analysis of gene expression profiles of OPN signalling pathway in four kinds of liver diseases

    Indian Academy of Sciences (India)

    GAIPING WANG; SHASHA CHEN; CONGCONG ZHAO; XIAOFANG LI; WEIMING ZHAO; JING YANG; CUIFANG CHANG; CUNSHUAN XU

    2016-09-01

    To explore the relevance of OPN signalling pathway to the occurrence and development of nonalcoholic fatty liver disease (NAFLD), liver cirrhosis (LC), hepatic cancer (HC) and acute hepatic failure (AHF) at transcriptional level, Rat Genome 230 2.0 Array was used to detect expression profiles of OPN signalling pathway-related genes in four kinds of liver diseases. The results showed that 23, 33, 59 and 74 genes were significantly changed in the above four kinds of liver diseases, respectively. H-clustering analysis showed that the expression profiles of OPN signalling-related genes were notably different in four kinds of liver diseases. Subsequently, a total of above-mentioned 147 genes were categorized into four clusters by k-means according to the similarity of gene expression, and expression analysis systematic explorer (EASE) functional enrichment analysis revealed that OPN signalling pathway-related genes were involved in cell adhesion and migration, cell proliferation, apoptosis, stress and inflammatory reaction, etc. Finally, ingenuity pathway analysis (IPA) software was used to predict thefunctions of OPN signalling-related genes, and the results indicated that the activities of ROS production, cell adhesion and migration, cell proliferation were remarkably increased, while that of apoptosis, stress and inflammatory reaction were reduced in four kinds of liver diseases. In summary, the above physiological activities changed more obviously in LC, HC and AHF than in NAFLD

  19. Comparative analysis of gene expression profiles of OPN signaling pathway in four kinds of liver diseases.

    Science.gov (United States)

    Wang, Gaiping; Chen, Shasha; Zhao, Congcong; Li, Xiaofang; Zhao, Weiming; Yang, Jing; Chang, Cuifang; Xu, Cunshuan

    2016-09-01

    To explore the relevance of OPN signalling pathway to the occurrence and development of nonalcoholic fatty liver disease (NAFLD), liver cirrhosis (LC), hepatic cancer (HC) and acute hepatic failure (AHF) at transcriptional level, Rat Genome 230 2.0 Array was used to detect expression profiles of OPN signalling pathway-related genes in four kinds of liver diseases. The results showed that 23, 33, 59 and 74 genes were significantly changed in the above four kinds of liver diseases, respectively. H-clustering analysis showed that the expression profiles of OPN signalling-related genes were notably different in four kinds of liver diseases. Subsequently, a total of above-mentioned 147 genes were categorized into four clusters by k-means according to the similarity of gene expression, and expression analysis systematic explorer (EASE) functional enrichment analysis revealed that OPN signalling pathway-related genes were involved in cell adhesion and migration, cell proliferation, apoptosis, stress and inflammatory reaction, etc. Finally, ingenuity pathway analysis (IPA) software was used to predict the functions of OPN signalling-related genes, and the results indicated that the activities of ROS production, cell adhesion and migration, cell proliferation were remarkably increased, while that of apoptosis, stress and inflammatory reaction were reduced in four kinds of liver diseases. In summary, the above physiological activities changed more obviously in LC, HC and AHF than in NAFLD.

  20. Gamma Astrometric Measurement Experiment (GAME) - Implementation and performance

    Science.gov (United States)

    Gai, Mario; Gai, Mario; Vecchiato, Alberto; Lattanzi, Mario G.; Ligori, Sebastiano; Loreggia, Davide

    The GAME mission concept is aimed at test of the General Relativity, through very precise measurement of the gravitational deflection of light by the Sun, by means of an optimised telescope operating in the visible and launched in orbit on a small class satellite. We recall the science motivations, discussed in detail in a separate contribution by Vecchiato et al., and describe the mission requirements derivation, the proposed mission profile, the preliminary payload design and the expected performance. The targeted precision on the "γ" parameter of the Parametrised Post-Newtonian formulation of General Relativity is in the range 10-6 to 10-7 or better, with an improvement of one or two orders of magnitude with respect to the best currently available experimental results. Such precision is suitable to detect possible deviations of γ from the unity value, associated to generalised Einstein models for gravitation, with potentially huge impacts on the cosmological distribution of dark matter and dark energy. The measurement principle is based on the differential astrometric signature on the stellar positions, i.e. on the spatial component of the gravitational effect, rather than the temporal component as in the most recent experiments based on radio link delay timing. Calibration is based on frequent measurement of angular separation of bright sources in stellar fields affected by negligible deflection. The instrument concept is based on a dual field, multiple aperture Fizeau interferometer, observing simultaneously two sky regions close to the Solar limb. A split flat mirror is used to fold the telescope line of sight on two different directions on the sky, separated by a base angle of about 4 degrees, which represents the gauge applied on the sky to measure the desired angular value of deflection. Stability or calibration of the base angle is the key to fulfilling the GAME science goals. An internal laser metrology option is considered for both on ground

  1. Microlensing Events in Gaia and other Astrometric Surveys

    Science.gov (United States)

    Baker, Claire; Di Stefano, Rosanne; Lepine, Sebastien

    2017-01-01

    The region within a kiloparsec of the Sun is a vast and mysterious place filled with uncharted planets, stars and compact objects, whose masses and properties are unknown. The Gaia space mission provides a unique opportunity to study of this region by measuring parallax distances and proper motions to millions of nearby stars, significantly advancing data available from previous astrometric surveys.We are putting this new astrometric information from the first Gaia data release to a novel use, by searching for matches between the positions of known microlensing events and the positions of stars observed by both the Gaia and the Tycho-2 missions, as listed in the Tycho-Gaia Astrometric Solution (TGAS) Catalogue.The existence of a gravitational microlensing event near a TGAS-listed star may provide information about the nature of either the source star lensed in the event, or the lens itself. For example, the source star lensed in the ‘TAGO’ event lies nearby, and is listed in the TGAS Catalogue. Other events may also have been caused by nearby TGAS-listed stars, or by their dim companions. In such cases, we can determine the lens mass and acquire information about any compact objects or planets which may exist around the lens.We report on the process of matching the positions of over 20,000 candidate microlensing events discovered by either OGLE and/or MOA, with the positions of 2 million stars from the TGAS Catalogue and stars from a range of other surveys, including Lepine's SUPERBLINK survey, and discuss the implications of the matches obtained.

  2. Precise Temporal Profiling of Signaling Complexes in Primary Cells Using SWATH Mass Spectrometry

    Directory of Open Access Journals (Sweden)

    Etienne Caron

    2017-03-01

    Full Text Available Spatiotemporal organization of protein interactions in cell signaling is a fundamental process that drives cellular functions. Given differential protein expression across tissues and developmental stages, the architecture and dynamics of signaling interaction proteomes is, likely, highly context dependent. However, current interaction information has been almost exclusively obtained from transformed cells. In this study, we applied an advanced and robust workflow combining mouse genetics and affinity purification (AP-SWATH mass spectrometry to profile the dynamics of 53 high-confidence protein interactions in primary T cells, using the scaffold protein GRB2 as a model. The workflow also provided a sufficient level of robustness to pinpoint differential interaction dynamics between two similar, but functionally distinct, primary T cell populations. Altogether, we demonstrated that precise and reproducible quantitative measurements of protein interaction dynamics can be achieved in primary cells isolated from mammalian tissues, allowing resolution of the tissue-specific context of cell-signaling events.

  3. Astrometric jitter of the sun as a star

    CERN Document Server

    Makarov, V V; Ulrich, R K

    2010-01-01

    The daily variation of the solar photocenter over some 11 years is derived from the Mount Wilson data reprocessed by Ulrich et al. 2010 to closely match the surface distribution of solar irradiance. The standard deviations of astrometric jitter are 0.52 $\\mu$AU and 0.39 $\\mu$AU in the equatorial and the axial dimensions, respectively. The overall dispersion is strongly correlated with the solar cycle, reaching $0.91 \\mu$AU at the maximum activity in 2000. The largest short-term deviations from the running average (up to 2.6 $\\mu$AU) occur when a group of large spots happen to lie on one side with respect to the center of the disk. The amplitude spectrum of the photocenter variations never exceeds 0.033 $\\mu$AU for the range of periods 0.6--1.4 yr, corresponding to the orbital periods of planets in the habitable zone. Astrometric detection of Earth-like planets around stars as quiet as the Sun is not affected by star spot noise, but the prospects for more active stars may be limited to giant planets.

  4. SPHERE IRDIS and IFS astrometric strategy and calibration

    CERN Document Server

    Maire, Anne-Lise; Dohlen, Kjetil; Lagrange, Anne-Marie; Gratton, Raffaele; Chauvin, Gael; Desidera, Silvano; Girard, Julien H; Milli, Julien; Vigan, Arthur; Zins, Gerard; Delorme, Philippe; Beuzit, Jean-Luc; Claudi, Riccardo U; Feldt, Markus; Mouillet, David; Puget, Pascal; Turatto, Massimo; Wildi, Francois

    2016-01-01

    We present the current results of the astrometric characterization of the VLT planet finder SPHERE over 2 years of on-sky operations. We first describe the criteria for the selection of the astrometric fields used for calibrating the science data: binaries, multiple systems, and stellar clusters. The analysis includes measurements of the pixel scale and the position angle with respect to the North for both near-infrared subsystems, the camera IRDIS and the integral field spectrometer IFS, as well as the distortion for the IRDIS camera. The IRDIS distortion is shown to be dominated by an anamorphism of 0.60+/-0.02% between the horizontal and vertical directions of the detector, i.e. 6 mas at 1". The anamorphism is produced by the cylindrical mirrors in the common path structure hence common to all three SPHERE science subsystems (IRDIS, IFS, and ZIMPOL), except for the relative orientation of their field of view. The current estimates of the pixel scale and North angle for IRDIS are 12.255+/-0.009 milliarcseco...

  5. Astrometry.net: Blind astrometric calibration of arbitrary astronomical images

    CERN Document Server

    Lang, Dustin; Mierle, Keir; Blanton, Michael; Roweis, Sam

    2009-01-01

    We have built a reliable and robust system that takes as input an astronomical image, and returns as output the pointing, scale, and orientation of that image (the astrometric calibration or WCS information). The system requires no first guess, and works with the information in the image pixels alone; that is, the problem is a generalization of the "lost in space" problem in which nothing--not even the image scale--is known. After robust source detection is performed in the input image, asterisms (sets of four or five stars) are geometrically hashed and compared to pre-indexed hashes to generate hypotheses about the astrometric calibration. A hypothesis is only accepted as true if it passes a Bayesian decision theory test against a background hypothesis. With indices built from the USNO-B Catalog and designed for uniformity of coverage and redundancy, the success rate is 99.9% for contemporary near-ultraviolet and visual imaging survey data, with no false positives. The failure rate is consistent with the inc...

  6. Astrometry.net: Blind Astrometric Calibration of Arbitrary Astronomical Images

    Science.gov (United States)

    Lang, Dustin; Hogg, David W.; Mierle, Keir; Blanton, Michael; Roweis, Sam

    2010-05-01

    We have built a reliable and robust system that takes as input an astronomical image, and returns as output the pointing, scale, and orientation of that image (the astrometric calibration or World Coordinate System information). The system requires no first guess, and works with the information in the image pixels alone; that is, the problem is a generalization of the "lost in space" problem in which nothing—not even the image scale—is known. After robust source detection is performed in the input image, asterisms (sets of four or five stars) are geometrically hashed and compared to pre-indexed hashes to generate hypotheses about the astrometric calibration. A hypothesis is only accepted as true if it passes a Bayesian decision theory test against a null hypothesis. With indices built from the USNO-B catalog and designed for uniformity of coverage and redundancy, the success rate is >99.9% for contemporary near-ultraviolet and visual imaging survey data, with no false positives. The failure rate is consistent with the incompleteness of the USNO-B catalog; augmentation with indices built from the Two Micron All Sky Survey catalog brings the completeness to 100% with no false positives. We are using this system to generate consistent and standards-compliant meta-data for digital and digitized imaging from plate repositories, automated observatories, individual scientific investigators, and hobbyists. This is the first step in a program of making it possible to trust calibration meta-data for astronomical data of arbitrary provenance.

  7. A study of purely astrometric selection of extragalactic point sources

    CERN Document Server

    Heintz, Kasper E; Høg, Erik

    2015-01-01

    Selection of extragalactic point sources, e.g. QSOs, is often hampered by significant selection effects causing existing samples to have rather complex selection functions. We explore whether a purely astrometric selection of extragalactic point sources, e.g. QSOs, is feasible with the ongoing Gaia mission. Such a selection would be interesting as it would be unbiased in terms of colours of the targets and hence would allow selection also with colours in the stellar sequence. We have analyzed a total of 18 representative regions of the sky by using \\textit{GUMS}, the simulator prepared for ESAs Gaia mission, both in the range of $12\\le G \\le 20$ mag and $12\\le G \\le 18$ mag. For each region we determine the density of apparently stationary stellar sources, i.e. sources for which Gaia cannot measure a significant proper motion. The density is contrasted with the density of extragalactic point sources, e.g. QSOs, in order to establish in which celestial directions a pure astrometric selection is feasible. When ...

  8. Protein signaling networks from single cell fluctuations and information theory profiling.

    Science.gov (United States)

    Shin, Young Shik; Remacle, F; Fan, Rong; Hwang, Kiwook; Wei, Wei; Ahmad, Habib; Levine, R D; Heath, James R

    2011-05-18

    Protein signaling networks among cells play critical roles in a host of pathophysiological processes, from inflammation to tumorigenesis. We report on an approach that integrates microfluidic cell handling, in situ protein secretion profiling, and information theory to determine an extracellular protein-signaling network and the role of perturbations. We assayed 12 proteins secreted from human macrophages that were subjected to lipopolysaccharide challenge, which emulates the macrophage-based innate immune responses against Gram-negative bacteria. We characterize the fluctuations in protein secretion of single cells, and of small cell colonies (n = 2, 3,···), as a function of colony size. Measuring the fluctuations permits a validation of the conditions required for the application of a quantitative version of the Le Chatelier's principle, as derived using information theory. This principle provides a quantitative prediction of the role of perturbations and allows a characterization of a protein-protein interaction network.

  9. Protein Signaling Networks from Single Cell Fluctuations and Information Theory Profiling

    Science.gov (United States)

    Shin, Young Shik; Remacle, F.; Fan, Rong; Hwang, Kiwook; Wei, Wei; Ahmad, Habib; Levine, R.D.; Heath, James R.

    2011-01-01

    Protein signaling networks among cells play critical roles in a host of pathophysiological processes, from inflammation to tumorigenesis. We report on an approach that integrates microfluidic cell handling, in situ protein secretion profiling, and information theory to determine an extracellular protein-signaling network and the role of perturbations. We assayed 12 proteins secreted from human macrophages that were subjected to lipopolysaccharide challenge, which emulates the macrophage-based innate immune responses against Gram-negative bacteria. We characterize the fluctuations in protein secretion of single cells, and of small cell colonies (n = 2, 3,···), as a function of colony size. Measuring the fluctuations permits a validation of the conditions required for the application of a quantitative version of the Le Chatelier's principle, as derived using information theory. This principle provides a quantitative prediction of the role of perturbations and allows a characterization of a protein-protein interaction network. PMID:21575571

  10. Profiling DNA damage-induced phosphorylation in budding yeast reveals diverse signaling networks.

    Science.gov (United States)

    Zhou, Chunshui; Elia, Andrew E H; Naylor, Maria L; Dephoure, Noah; Ballif, Bryan A; Goel, Gautam; Xu, Qikai; Ng, Aylwin; Chou, Danny M; Xavier, Ramnik J; Gygi, Steven P; Elledge, Stephen J

    2016-06-28

    The DNA damage response (DDR) is regulated by a protein kinase signaling cascade that orchestrates DNA repair and other processes. Identifying the substrate effectors of these kinases is critical for understanding the underlying physiology and mechanism of the response. We have used quantitative mass spectrometry to profile DDR-dependent phosphorylation in budding yeast and genetically explored the dependency of these phosphorylation events on the DDR kinases MEC1, RAD53, CHK1, and DUN1. Based on these screens, a database containing many novel DDR-regulated phosphorylation events has been established. Phosphorylation of many of these proteins has been validated by quantitative peptide phospho-immunoprecipitation and examined for functional relevance to the DDR through large-scale analysis of sensitivity to DNA damage in yeast deletion strains. We reveal a link between DDR signaling and the metabolic pathways of inositol phosphate and phosphatidyl inositol synthesis, which are required for resistance to DNA damage. We also uncover links between the DDR and TOR signaling as well as translation regulation. Taken together, these data shed new light on the organization of DDR signaling in budding yeast.

  11. Light-weight single trial EEG signal processing algorithms: computational profiling for low power design.

    Science.gov (United States)

    Ahmadi, Ali; Jafari, Roozbeh; Hart, John

    2011-01-01

    Brain Computer Interface (BCI) systems translate brain rhythms into signals comprehensible by computers. BCI has numerous applications in the clinical domain, the computer gaming, and the military. Real-time analysis of single trial brain signals is a challenging task, due to the low SNR of the incoming signals, added noise due to muscle artifacts, and trial-to-trial variability. In this work we present a computationally lightweight classification method based on several time and frequency domain features. After preprocessing and filtering, wavelet transform and Short Time Fourier Transform (STFT) are used for feature extraction. Feature vectors which are extracted from θ and α frequency bands are classified using a Support Vector Machine (SVM) classifier. EEG data were recorded from 64 electrodes during three different Go/NoGo tasks. We achieved 91% classification accuracy for two-class discrimination. The high recognition rate and low computational complexity makes this approach a promising method for a BCI system running on wearable and mobile devices. Computational profiling shows that this method is suitable for real time signal processing implementation.

  12. Transcriptional gene expression profiles of HGF/SF-met signaling pathway in colorectal carcinoma

    Institute of Scientific and Technical Information of China (English)

    Xue-Nong Li; Yan-Qing Ding; Guo-Bing Liu

    2003-01-01

    AIM: To explore the transcriptional gene expression profiles of HGF/SF-met signaling pathway in colorectal carcinoma to understand mechanisms of the signaling pathway at so gene level.METHODS: Total RNA was isolated from human colorectal carcinoma cell line LoVo treated with HGF/SF (80 ng/L)for 48 h. Fluorescent probes were prepared from RNA labeled with cy3-dUTP for the control groups and with cy5-dUTP for the HGF/SF-treated groups through reversetranscription. The probes were mixed and hybridized on the microarray at 60 ℃ for 15-20 h, then the microarray was scanned by laser scanner (GenePix 4000B). The intensity of each spot and ratios of Cy5/Cy3 were analyzed and finally the differentially expressed genes were selected by GenePix Pro 3.0 software. 6 differential expression genes (3 up-regulated genes and 3 down-regulated genes) were selected randomly and analyzed by β-actin semiquantitative RT-PCR.RESULTS: The fluorescent intensities of built-in negative control spots were less than 200, and the fluorescent intensities of positive control spots were more than 5000.Of the 4004 human genes analyzed by microarray, 129 genes (holding 3.22 % of the investigated genes) revealed differential expression in HGF/SF-treated groups compared with the control groups, of which 61 genes were up-regulated (holding 1.52 % of the investigated genes) and 68 genes were down-regulated (holding 1.70 % of the investigated genes), which supplied abundant information about target genes of HGF/SF-met signaling.CONCLUSION: HGF/SF-met signaling may up-regulate oncogenes, signal transduction genes, apoptosis-related genes, metastasis related genes, and down-regulate a number of genes. The complexity of HGF/SF-met signaling to control the gene expression is revealed as a whole by the gene chip technology.

  13. Signaling pathway-focused gene expression profiling in pressure overloaded hearts

    Directory of Open Access Journals (Sweden)

    Marco Musumeci

    2011-01-01

    Full Text Available The β-blocker propranolol displays antihypertrophic and antifibrotic properties in the heart subjected to pressure overload. Yet the underlying mechanisms responsible for these important effects remain to be completely understood. The purpose of this study was to determine signaling pathway-focused gene expression profile associated with the antihypertrophic action of propranolol in pressure overloaded hearts. To address this question, a focused real-time PCR array was used to screen left ventricular RNA expression of 84 gene transcripts representative of 18 different signaling pathways in C57BL/6 mice subjected to transverse aortic constriction (TAC or sham surgery. On the surgery day, mice received either propranolol (80 mg/kg/day or vehicle for 14 days. TAC caused a 49% increase in the left ventricular weight-to-body weight (LVW/BW ratio without changing gene expression. Propranolol blunted LVW/BW ratio increase by approximately 50% while causing about a 3-fold increase in the expression of two genes, namely Brca1 and Cdkn2a, belonging to the TGF-beta and estrogen pathways, respectively. In conclusion, after 2 weeks of pressure overload, TAC hearts show a gene expression profile superimposable to that of sham hearts. Conversely, propranolol treatment is associated with an increased expression of genes which negatively regulate cell cycle progression. It remains to be established whether a mechanistic link between gene expression changes and the antihypertrophic action of propranolol occurs.

  14. Comparative analysis of fertility signals and sex-specific cuticular chemical profiles of Odontomachus trap-jaw ants.

    Science.gov (United States)

    Smith, Adrian A; Millar, Jocelyn G; Suarez, Andrew V

    2016-02-01

    The lipid mixture that coats the insect cuticle contains a number of chemical signals. Mate choice in solitary insects is mediated by sexually dimorphic cuticular chemistry, whereas in eusocial insects, these profiles provide information through which colony members are identified and the fertility status of individuals is assessed. Profiles of queens and workers have been described for a number of eusocial species, but there have been few comparisons of fertility signals among closely related species. Additionally, sexual dimorphism in cuticular lipid profiles has only been reported in two species of ants. This study describes the cuticular chemical profiles of queens, workers and males of three species of Odontomachus trap-jaw ants: O. ruginodis, O. relictus and O. haematodus. These are compared with fertility signals and sexually dimorphic profiles already described from O. brunneus. We report that fertility signals are not conserved within this genus: chemical compounds that distinguish queens from workers vary in number and type among the species. Furthermore, the compounds that were most abundant in cuticular extracts of O. ruginodis queens relative to workers were novel 2,5-dialkyltetrahydrofurans. Bioassays of extracts of O. ruginodis queens indicate that the dialkyltetrahydrofuran and hydrocarbon fractions of the profile are likely to work synergistically in eliciting behavioral responses from workers. In contrast, cuticular lipids that distinguish males from females are more conserved across species, with isomeric and relative abundance variations comprising the main differences among species. Our results provide new insights into how these contact chemical signals may have arisen and evolved within eusocial insects.

  15. Whole Genome Expression Profiling and Signal Pathway Screening of MSCs in Ankylosing Spondylitis

    Directory of Open Access Journals (Sweden)

    Yuxi Li

    2014-01-01

    Full Text Available The pathogenesis of dysfunctional immunoregulation of mesenchymal stem cells (MSCs in ankylosing spondylitis (AS is thought to be a complex process that involves multiple genetic alterations. In this study, MSCs derived from both healthy donors and AS patients were cultured in normal media or media mimicking an inflammatory environment. Whole genome expression profiling analysis of 33,351 genes was performed and differentially expressed genes related to AS were analyzed by GO term analysis and KEGG pathway analysis. Our results showed that in normal media 676 genes were differentially expressed in AS, 354 upregulated and 322 downregulated, while in an inflammatory environment 1767 genes were differentially expressed in AS, 1230 upregulated and 537 downregulated. GO analysis showed that these genes were mainly related to cellular processes, physiological processes, biological regulation, regulation of biological processes, and binding. In addition, by KEGG pathway analysis, 14 key genes from the MAPK signaling and 8 key genes from the TLR signaling pathway were identified as differentially regulated. The results of qRT-PCR verified the expression variation of the 9 genes mentioned above. Our study found that in an inflammatory environment ankylosing spondylitis pathogenesis may be related to activation of the MAPK and TLR signaling pathways.

  16. Context-specificity in causal signaling networks revealed by phosphoprotein profiling

    Science.gov (United States)

    Hill, Steven M.; Nesser, Nicole K.; Johnson-Camacho, Katie; Jeffress, Mara; Johnson, Aimee; Boniface, Chris; Spencer, Simon E. F.; Lu, Yiling; Heiser, Laura M.; Lawrence, Yancey; Pande, Nupur T.; Korkola, James E.; Gray, Joe W.; Mills, Gordon B.; Mukherjee, Sach; Spellman, Paul T.

    2017-01-01

    Summary Signaling networks downstream of receptor tyrosine kinases are among the most extensively studied biological networks, but new approaches are needed to elucidate causal relationships between network components and understand how such relationships are influenced by biological context and disease. Here, we investigate the context-specificity of signaling networks within a causal conceptual framework, using reverse-phase protein array time-course assays and network analysis approaches. We focus on a well-defined set of signaling proteins profiled under inhibition with five kinase inhibitors in 32 contexts—four breast cancer cell lines (MCF7, UACC812, BT20, and BT549) under eight stimulus conditions. The data, spanning multiple pathways and comprising ~70,000 phosphoprotein and ~260,000 protein measurements, provide a wealth of testable, context-specific hypotheses, several of which we experimentally validate. Furthermore, the data provide a unique resource for computational methods development, permitting empirical assessment of causal network learning in a complex, mammalian setting. PMID:28017544

  17. The science, technology and mission design for the Laser Astrometric test of relativity

    Science.gov (United States)

    Turyshev, Slava G.

    2006-01-01

    The Laser Astrometric Test of Relativity (LATOR) is a Michelson-Morley-type experiment designed to test the Einstein's general theory of relativity in the most intense gravitational environment available in the solar system - the close proximity to the Sun.

  18. The astrometric core solution for the Gaia mission. Overview of models, algorithms and software implementation

    CERN Document Server

    Lindegren, Lennart; Hobbs, David; O'Mullane, William; Bastian, Ulrich; Hernández, José

    2011-01-01

    The Gaia satellite will observe about one billion stars and other point-like sources. The astrometric core solution will determine the astrometric parameters (position, parallax, and proper motion) for a subset of these sources, using a global solution approach which must also include a large number of parameters for the satellite attitude and optical instrument. The accurate and efficient implementation of this solution is an extremely demanding task, but crucial for the outcome of the mission. We provide a comprehensive overview of the mathematical and physical models applicable to this solution, as well as its numerical and algorithmic framework. The astrometric core solution is a simultaneous least-squares estimation of about half a billion parameters, including the astrometric parameters for some 100 million well-behaved so-called primary sources. The global nature of the solution requires an iterative approach, which can be broken down into a small number of distinct processing blocks (source, attitude,...

  19. Implementation of a Novel Algorithm on Acoustic Doppler Current Profiler Signal Processing System

    Institute of Scientific and Technical Information of China (English)

    ZHU Hao; LIU Wenyao

    2005-01-01

    Acoustic Doppler current profiler (ADCP) uses acoustic energy directed along narrow beams for current measurement. In conventional method, the quantity of sampling affects the precision of fast Fourier transform (FFT) algorithm, and the algorithm needs a large amount of data to process. A novel frequency estimator,enhanced least mean square (ELMS) algorithm for a single complex sinusoid in complex white Gaussian noise, is proposed in ADCP system. As sampling frequency equals 120 krad/s and the sampling number equals 240, the minimum resolving is 0.5 krad/s. All variances keep 11.11%. ELMS algorithm needs less data than FFT. And the robust algorithm can estimate the spectrum true value to 99.9% when the signal to noise ratio (SNR) is equal to 0 dB. Experiments prove that the estimation values will diverge much from the ideal when SNR is less than -6 dB.

  20. Personalized evolutionary hypothesis in genomics and auxiliary lymph node through diverse subtelomeric signal profile.

    Science.gov (United States)

    Mehdipour, Parvin; Javan, Firoozeh; Savad, Shahram; Karbassian, Hamid; Atri, Morteza

    2015-01-24

    Few available data on the genomic-somatic evolution in breast cancer create limitation to provide the appropriate clinical managements. As an example, human subtelomeres (ST) are diverse-prone and variable targets. STs, as hot spots, have positive and negative impacts on the status of health and malady. We showed higher subtelomere signal copy number (SCN) of specific chromosomes in genomics than in auxiliary lymph node (ALN). Dissimilarity of signal intensity (SI) is found for all chromosomes. Significantly higher SI in genomics than in ALN cells were specified as chromosomes 5, 6, 9-12, 16-19 for weak; 1, 5-9, 19, X for medium; and 2, 5, 9, 10, 16, 18 for strong SI. For lacking, and presence of one and two SCNs; p/q ratio reflected differences for all chromosomes; but, 2, 3, 5, 7, 8, 10, 16, 18, 20, and X chromosomes were involved for three SCN. Chromosomes 1, 4, 9, 12, 17-19 lacked three SCN in ALN and lymphocytes. Weak SI ratio was higher in p- than in q-arm in majority of chromosomes. Manner of evolution and diversity in p- and q-arms is expressive of a novel definition as two diverse domains with a personalized insight. These data have been accompanied by periodic charts as ST array profiles which provide specific and individualized pattern in breast neoplasm. Such profiling at genomics level could be considered as a prediction through the patients' life. Moreover, subtelomere territory by interacting with protein expression of Ki67, cyclin D1, and cyclin E; and molecular targets including telomere length at genomics and somatic level provides package of information to bridge cancer cell biology to the cancer clinic as "puzzling paradigm." © 2015 International Federation for Cell Biology.

  1. Experimental Tests of the Astrometric Precision Obtainable with a Ten Micron Interferometer.

    Science.gov (United States)

    1982-01-18

    declination-dependent phase offset is caused by the failure of the axes of rotation of the heliostat mirrors to intersect precisely . The source...A-Ji>L __ _ _ _ _ _ 4. TITLE (and Subtitle) S. TYPE Of REPORT & PERIOD COVERED EXPERIMENTAL TESTS OF THE ASTROMETRIC PRECISION Final Contract...of tests successfully achieved its objective and demonstrated the usefulness of infrared spatial interferometry for very precise astrometric

  2. Comparative expression profiling identifies differential roles for Myogenin and p38α MAPK signaling in myogenesis

    Institute of Scientific and Technical Information of China (English)

    Qi-Cai Liu; Marjorie Brand; Carol Perez-Iratxeta; F. Jeffrey Dilworth; Xiao-Hui Zha; Hervé Faralli; Hang Yin; Caroline Louis-Jeune; Eusebio Perdiguero; Erinija Pranckeviciene; Pura Mu(n)oz-Cànoves; Michael A. Rudnicki

    2012-01-01

    Skeletal muscle differentiation is mediated by a complex gene expression program requiring both the muscle-specific transcription factor Myogenin (Myog) and p38α MAPK (p38α) signaling.However,the relative contribution of Myog and p38α to the formation of mature myotubes remains unknown.Here,we have uncoupled the activity of Myog from that of p38α to gain insight into the individual roles of these proteins in myoganesis.Comparative expression profiling confirmed that Myog activates the expression of genes involved in muscle function.Furthermore,we found that in the absence of p38α signaling,Myog expression leads to the down-regulation of genes involved in cell cycle progression.Consistent with this,the expression of Myog is sufficient to induce cell cycle exit.Interestingly,p38α-defective,Myog-expressing myoblasts fail to form multinucleated myotubes,suggesting an important role for p38α in cell fusion.Through the analysis of p38α up-regulated genes,the tetraspanin CD53 was identified as a candidate fusion protein,a role confirmed both ex vivo in primary myoblasts,and in vivo during myofiber regeneration in mice.Thus,our study has revealed an unexpected role for Myog in mediating cell cycle exit and has identified an essential role for p38α in cell fusion through the up-regulation of CD53.

  3. Profiling of anti-fibrotic signaling by hepatocyte growth factor in renal fibroblasts

    Energy Technology Data Exchange (ETDEWEB)

    Schievenbusch, Stephanie; Strack, Ingo; Scheffler, Melanie; Wennhold, Kerstin; Maurer, Julia [Institute for Pathology, University Hospital Cologne, Kerpener Str. 62, 50924 Koeln (Germany); Nischt, Roswitha [Department of Dermatology, University Hospital of Cologne (Germany); Dienes, Hans Peter [Institute for Pathology, University Hospital Cologne, Kerpener Str. 62, 50924 Koeln (Germany); Odenthal, Margarete, E-mail: m.odenthal@uni-koeln.de [Institute for Pathology, University Hospital Cologne, Kerpener Str. 62, 50924 Koeln (Germany)

    2009-07-17

    Hepatocyte growth factor (HGF) is a multifunctional growth factor affecting cell proliferation and differentiation. Due to its mitogenic potential, HGF plays an important role in tubular repair and regeneration after acute renal injury. However, recent reports have shown that HGF also acts as an anti-inflammatory and anti-fibrotic factor, affecting various cell types such as renal fibroblasts and triggering tubulointerstitial fibrosis of the kidney. The present study provides evidence that HGF stimulation of renal fibroblasts results in the activation of both the Erk1/2 and the Akt pathways. As previously shown, Erk1/2 phosphorylation results in Smad-linker phosphorylation, thereby antagonizing cellular signals induced by TGF{beta}. By siRNA mediated silencing of the Erk1/2-Smad linkage, however, we now demonstrate that Akt signaling acts as an auxiliary pathway responsible for the anti-fibrotic effects of HGF. In order to define the anti-fibrotic function of HGF we performed comprehensive expression profiling of HGF-stimulated renal fibroblasts by microarray hybridization. Functional cluster analyses and quantitative PCR assays indicate that the HGF-stimulated pathways transfer the anti-fibrotic effects in renal interstitial fibroblasts by reducing expression of extracellular matrix proteins, various chemokines, and members of the CCN family.

  4. Expression profile and function of Wnt signaling mechanisms in malignant mesothelioma cells

    Energy Technology Data Exchange (ETDEWEB)

    Fox, Simon A., E-mail: s.fox@curtin.edu.au [Molecular Pharmacology Laboratory, School of Pharmacy, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA (Australia); Richards, Alex K.; Kusumah, Ivonne; Perumal, Vanathi [Molecular Pharmacology Laboratory, School of Pharmacy, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA (Australia); Bolitho, Erin M. [Western Australian Institute for Medical Research, The University of Western Australia Centre for Medical Research, Perth, WA (Australia); Mutsaers, Steven E. [Lung Institute of Western Australia, Centre for Asthma Allergy and Respiratory Research, University of Western Australia, Nedlands (Australia); Centre for Cell Therapy and Regenerative Medicine, School of Medicine and Pharmacology, University of Western Australia and Western Australian Institute for Medical Research, Nedlands (Australia); Dharmarajan, Arun M. [School of Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA (Australia)

    2013-10-11

    Highlights: •Expression profile of Wnt pathway related genes in mesothelioma cells. •Differential expression of key Wnt pathway molecules and regulators. •Wnt3a stimulated mesothelioma growth whereas sFRP4 was inhibitory. •Targeting β-Catenin can sensitise mesothelioma cells to cytotoxic drugs. -- Abstract: Malignant mesothelioma (MM) is an uncommon and particularly aggressive cancer associated with asbestos exposure, which currently presents an intractable clinical challenge. Wnt signaling has been reported to play a role in the neoplastic properties of mesothelioma cells but has not been investigated in detail in this cancer. We surveyed expression of Wnts, their receptors, and other key molecules in this pathway in well established in vitro mesothelioma models in comparison with primary mesothelial cultures. We also tested the biological response of MM cell lines to exogenous Wnt and secreted regulators, as well as targeting β-catenin. We detected frequent expression of Wnt3 and Wnt5a, as well as Fzd 2, 4 and 6. The mRNA of Wnt4, Fzd3, sFRP4, APC and axin2 were downregulated in MM relative to mesothelial cells while LEF1 was overexpressed in MM. Functionally, we observed that Wnt3a stimulated MM proliferation while sFRP4 was inhibitory. Furthermore, directly targeting β-catenin expression could sensitise MM cells to cytotoxic drugs. These results provide evidence for altered expression of a number of Wnt/Fzd signaling molecules in MM. Modulation of Wnt signaling in MM may prove a means of targeting proliferation and drug resistance in this cancer.

  5. New constraints on Saturn's interior from Cassini astrometric data

    CERN Document Server

    Lainey, Valéry; Tajeddine, Radwan; Cooper, Nicholas J; Murray, Carl; Robert, Vincent; Tobie, Gabriel; Guillot, Tristan; Mathis, Stéphane; Remus, Françoise; Desmars, Josselin; Arlot, Jean-Eudes; De Cuyper, Jean-Pierre; Dehant, Véronique; Pascu, Dan; Thuillot, William; Poncin-Lafitte, Christophe Le; Zahn, Jean-Paul

    2015-01-01

    Using astrometric observations spanning more than a century and including a large set of Cassini data, we determine Saturn's tidal parameters through their current effects on the orbits of the eight main and four coorbital moons. We have used the latter to make the first determination of Saturn's Love number, $k_2=0.390 \\pm 0.024$, a value larger than the commonly used theoretical value of 0.341 (Gavrilov & Zharkov, 1977), but compatible with more recent models (Helled & Guillot, 2013) for which $k_2$ ranges from 0.355 to 0.382. Depending on the assumed spin for Saturn's interior, the new constraint can lead to a reduction of up to 80% in the number of potential models, offering great opportunities to probe the planet's interior. In addition, significant tidal dissipation within Saturn is confirmed (Lainey et al., 2012) corresponding to a high present-day tidal ratio $k_2/Q=(1.59 \\pm 0.74) \\times 10^{-4}$ and implying fast orbital expansions of the moons. This high dissipation, with no obvious variati...

  6. Fundamental Physics with the Laser Astrometric Test Of Relativity

    CERN Document Server

    Turyshev, S G; Lämmerzahl, C; Theil, S; Ertmer, W; Rasel, E; Foerstner, R; Johann, U; Klioner, S A; Soffel, M H; Dachwald, B; Seboldt, W; Perlick, V; Sandford, M C W; Bingham, R; Kent, B; Sumner, T J; Bertolami, O; Páramos, J; Christophe, B; Foulon, B; Touboul, P; Bouyer, P; Damour, T; Salomon, C; Reynaud, S; Brillet, A; Bondu, F; Mangin, J F; Samain, E; Bertotti, B; Iess, L; Erd, C; Grenouilleau, J C; Izzo, D; Rathke, A; Asmar, S W; Colavita, M; Gursel, Y; Hemmati, H; Shao, M; Williams, J G; Nordtvedt, K L; Shapiro, I; Reasenberg, R; Drever, R W P; Degnan, J; Plowman, J E; Hellings, R; Murphy, T W; Rovisco Pais, A; Copernic, A N; Favata, F; Turyshev, Slava G.; Dittus, Hansjoerg

    2005-01-01

    The Laser Astrometric Test Of Relativity (LATOR) is a European-U.S. Michelson-Morley-type experiment designed to test the pure tensor metric nature of gravitation - the fundamental postulate of Einstein's theory of general relativity. By using a combination of independent time-series of highly accurate gravitational deflection of light in the immediate proximity to the Sun along with measurements of the Shapiro time delay on the interplanetary scales (to a precision respectively better than 0.1 picoradians and 1 cm), LATOR will significantly improve our knowledge of relativistic gravity. The primary mission objective is to i) measure the key post-Newtonian Eddington parameter \\gamma with accuracy of a part in 10^9. (1-\\gamma) is a direct measure for presence of a new interaction in gravitational theory, and, in its search, LATOR goes a factor 30,000 beyond the present best result, Cassini's 2003 test. The mission will also provide: ii) first measurement of gravity's non-linear effects on light to ~0.01% accur...

  7. Photometric and astrometric vagaries of the enigma star KIC 8462852

    CERN Document Server

    Makarov, Valeri V

    2016-01-01

    We apply a PCA-based pre-whitening method to the entire collection of main Kepler mission long-cadence data for KIC 8462852 spanning four years. This technique removes the correlated variations of instrumental origin in both the detected light curves and astrometry, resolving intrinsic changes in flux and image position of less than 100 ppm and 1 mas, respectively. Beside the major dips in the light curve during mission quarters 8 and 16, when the flux dropped by up to 20%, we confirm multiple smaller dips across the time span of observation with amplitudes ranging from 0.1% to 7%. A variation of flux with a period of 0.88 d and a half-amplitude of approximately 90 ppm is confirmed in the PCA-cleaned data. We find that the phase of the wave is steady over the entire 15-month interval. We confidently detect a weak variability-induced motion (VIM) effect in the cleaned astrometric trajectories, when the moment-based centroids shift synchronously with the flux dips by up to 0.0008 pixels on the detector. The inc...

  8. Astrometric positioning and orbit determination of geostationary satellites

    Science.gov (United States)

    Montojo, F. J.; López Moratalla, T.; Abad, C.

    2011-03-01

    In the project titled “Astrometric Positioning of Geostationary Satellite” (PASAGE), carried out by the Real Instituto y Observatorio de la Armada (ROA), optical observation techniques were developed to allow satellites to be located in the geostationary ring with angular accuracies of up to a few tenths of an arcsec. These techniques do not necessarily require the use of large telescopes or especially dark areas, and furthermore, because optical observation is a passive method, they could be directly applicable to the detection and monitoring of passive objects such as space debris in the geostationary ring.By using single-station angular observations, geostationary satellite orbits with positional uncertainties below 350 m (2 sigma) were reconstructed using the Orbit Determination Tool Kit software, by Analytical Graphics, Inc. This software is used in collaboration with the Spanish Instituto Nacional de Técnica Aeroespacial.Orbit determination can be improved by taking into consideration the data from other stations, such as angular observations alone or together with ranging measurements to the satellite. Tests were carried out combining angular observations with the ranging measurements obtained from the Two-Way Satellite Time and Frequency Transfer technique that is used by ROA’s Time Section to carry out time transfer with other laboratories. Results show a reduction of the 2 sigma uncertainty to less than 100 m.

  9. GAME - A small mission concept for high-precision astrometric test of General Relativity

    Science.gov (United States)

    Vecchiato, A.; Gai, Mario; Donati, Paolo; Morbidelli, Roberto; Lattanzi, Mario G.; Crosta, Mariateresa

    2010-11-01

    GAME (Gamma Astrometric Measurement Experiment) is a concept for a small mission whose main goal is to measure from space the γ parameter of the Parameterized Post-Newtonian formalism, Will (2001)) A satellite, looking as close as possible to the Solar limb, measures the gravitational bending of light in a way similar to that followed by past experiments from the ground during solar eclipses. In the cited formalism, deviations of the γ parameter from unity are interpreted as deviations from the predictions of General Relativity which are foreseen by several competing theories of gravity. In the present theoretical scenario, such deviations are expected to appear in the range between 10-5 and 10-7. The most stringent experimental constraints available up to now are those of the Cassini mission, that gives 1-γ≲10-5 Bertotti et al. (2003), while future space missions are expected to reach the 10-7 level of accuracy. (Vecchiato et al. (2003), Turyshev et al. (2004), Ni (2008)) Preliminary simulations have shown that the expected final accuracy of GAME can reach the 10-7 level, or better if the mission profile can be extended to fit a larger budget Vecchiato et al. (2009), Gai et al. (2009). This work, which has recently been extended to better assess the mission performances, has confirmed the previous results and has given indications on how further improve various aspects of the mission profile. Moreover, thanks to its flexible observation strategy, GAME is also able to target other interesting scientific goals in the realm of General Relativity, as well as in those involving observations of selected extrasolar systems in the brown dwarf and planetary regime.

  10. Profile of select hepatic insulin signaling pathway genes in response to 2-aminoanthracene dietary ingestion.

    Science.gov (United States)

    Mattis, N D; Jay, J W; Barnett, G W; Rosaldo, J J; Howerth, E W; Means, J C; Gato, W E

    2014-01-01

    Some genes that regulate various processes such as insulin signaling, glucose metabolism, fatty acid, and lipid biosynthesis were profiled. The objective of the current investigation is to examine the mRNA expression of some genes that mediate insulin signaling due to 2AA toxicity. 2AA is a polycyclic aromatic hydrocarbon (PAH) that has been detected in broiled food and tobacco smoke. Twenty-four post-weaning 3-4-week-old F344 male rats were exposed to 0 mg/kg-diet, 50 mg/kg-diet, 75 mg/kg-diet, and 100 mg/kgdiet 2AA for 2 weeks and 4 weeks. The mRNA expression of AKT1, G6PC, GCK, GLUT4, INSR, IRS1, PP1R3C, PAMPK, SOCS 2, and SREBF1 was determined by qRTPCR followed by the quantification of G6PC and AMPK via ELISA. The results suggest that 2AA modulates these genes depending on the length of exposure. Up-regulation of AMPK and SOCS2 genes in animals treated with 100 mg/kg-diet and 50 mg/kg-diet, respectively, during 14 days of feeding was noted. G6PC expression was inhibited in the 2-week group while being dose-dependently increased in the 4-week group. Hepatic activity of G6PC was enhanced significantly in the livers of rats that ingested 2AA. It appears that 2AA intoxication leads to the activation of irs1 and akt1 genes in the liver. Quantified AMPK amounts increased significantly in the short-term treatment group. Dose-dependent rise of AMPK in animals treated to 2AA showed an increased production of hepatic AMPK in response to the toxicity of 2AA in order to maintain cellular homeostasis. In contrast, the reduction in AMPK concentration in treated animals within the 4-week set indicated an adaptive recovery.

  11. Revisiting TW Hydrae in light of new astrometric data

    Science.gov (United States)

    Teixeira, R.; Ducourant, C.; Galli, P. A. B.; Le Campion, J. F.; Zuckerman, B.; Krone-Martins, A. G. O.; Chauvin, G.; Song, I.

    2014-10-01

    Our efforts in the present work focused mainly on refining and improving the previous description and understanding of the stellar association TW Hydrae (TWA) including a very detailed membership analysis and its dynamical and evolutionary age.To achieve our objectives in a fully reliable way we take advantage of our own astrometric measurements (Ducourant et al. 2013) performed with NTT/EFOSC2 - ESO (La Silla - Chile) spread over three years (2007 - 2010) and of those published in the literature.A very detailed membership analysis based on the convergent point strategy as developed by our team (Galli et al. 2012, 2013) allowed us to define a consistent kinematic group containing 31 stars among the 44 proposed as TWA member in the literature. Assuming that our sample of stars may be contaminated by non-members and to get rid of the particular influence of each star we applied a Jacknife resampling technique generating 2000 random lists of 13 stars taken from our 16 stars and calculated for each the epoch of convergence when the radius is minimum. The mean of the epochs obtained and the dispersion about the mean give a dynamical age of 7.5± 0.7 Myr for the association that is in good agreement with the previous traceback age (De La Reza et al. 2006). We also estimated age for TWA moving group members from pre-main sequence evolutionary models (Siess et al. 2000) and find a mean age of 7.4± 1.2 Myr. These results show that the dynamical age of the association obtained via the traceback technique and the average age derived from theoretical evolutionary models are in good agreement.

  12. Improving the Planetary Ephemeris with Astrometric Observations of Cassini

    Science.gov (United States)

    Jones, Dayton L.; Fomalont, E.; Dhawan, V.; Romney, J.; Lanyi, G.; Border, J.; Folkner, B.; Jacobson, B.

    2009-05-01

    During the past three years we have carried out a series of astrometric VLBA observations of the Cassini spacecraft. At each epoch, we used phase referencing to obtain high precision relative positions between Cassini and an angularly nearby calibration source. The calibration sources were separately tied to nearby defining sources of the International Celestial Reference Frame (ICRF) through additional phase-referenced VLBA experiments. By combining our position measurements of Cassini with a model of Cassini's orbit around Saturn (from Doppler measurements by the Deep Space Network), we are able to determine the ICRF position of Saturn at each epoch to about 0.3 mas. This is about 2 km at the average distance of Saturn. These results will improve the Saturn ephemeris, particularly in ecliptic latitude (the plane of Saturn's orbit). The error in latitude decreases dramatically as the total time span of VLBA data approaches 1/4 of Saturn's orbital period in 2011-2012. Saturn is the first outer planet whose ephemeris can be improved and more closely tied to the ICRF and the inner solar system through long-term observations of an orbiter. The planned Juno mission to Jupiter will allow this technique to be applied there also. The planetary ephemeris is an essential tool for studies of solar system dynamics, interplanetary spacecraft navigation, and test of general relativity. It requires continuous maintenance and improvement. This research has been carried out at the Jet Propulsion Laboratory, California Institute of Technology, under contract with the National Aeronautics and Space Administration, and has relied on observations by the Very Long Baseline Array, a facility of the National Science Foundation operated by the National Radio Astronomy Observatory under a cooperative agreement with Associated Universities, Inc.

  13. New constraints on Saturn's interior from Cassini astrometric data

    Science.gov (United States)

    Lainey, Valéry; Jacobson, Robert A.; Tajeddine, Radwan; Cooper, Nicholas J.; Murray, Carl; Robert, Vincent; Tobie, Gabriel; Guillot, Tristan; Mathis, Stéphane; Remus, Françoise; Desmars, Josselin; Arlot, Jean-Eudes; De Cuyper, Jean-Pierre; Dehant, Véronique; Pascu, Dan; Thuillot, William; Le Poncin-Lafitte, Christophe; Zahn, Jean-Paul

    2017-01-01

    Using astrometric observations spanning more than a century and including a large set of Cassini data, we determine Saturn's tidal parameters through their current effects on the orbits of the eight main and four coorbital Moons. We have used the latter to make the first determination of Saturn's Love number from observations, k2=0.390 ± 0.024, a value larger than the commonly used theoretical value of 0.341 (Gavrilov & Zharkov, 1977), but compatible with more recent models (Helled & Guillot, 2013) for which the static k2 ranges from 0.355 to 0.382. Depending on the assumed spin for Saturn's interior, the new constraint can lead to a significant reduction in the number of potential models, offering great opportunities to probe the planet's interior. In addition, significant tidal dissipation within Saturn is confirmed (Lainey et al., 2012) corresponding to a high present-day tidal ratio k2/Q=(1.59 ± 0.74) × 10-4 and implying fast orbital expansions of the Moons. This high dissipation, with no obvious variations for tidal frequencies corresponding to those of Enceladus and Dione, may be explained by viscous friction in a solid core, implying a core viscosity typically ranging between 1014 and 1016 Pa.s (Remus et al., 2012). However, a dissipation increase by one order of magnitude at Rhea's frequency could suggest the existence of an additional, frequency-dependent, dissipation process, possibly from turbulent friction acting on tidal waves in the fluid envelope of Saturn (Ogilvie & Lin, 2004; Fuller et al. 2016).

  14. Tyramide Signal Amplification for Antibody-overlay Lectin Microarray: A Strategy to Improve the Sensitivity of Targeted Glycan Profiling

    Science.gov (United States)

    Meany, Danni L.; Hackler, Laszlo; Zhang, Hui; Chan, Daniel W.

    2011-01-01

    Antibody-overlay lectin microarray (ALM) has been used for targeted glycan profiling to identify disease-related protein glycoforms. In this context, high sensitivity is desired because it allows for the identification of disease-related glycoforms that are often present at low concentration. We describe a new Tyramide Signal Amplification (TSA) for Antibody-overlay Lectin Microarray procedure for sensitive profiling of glycosylation patterns. We demonstrated that TSA increased the sensitivity of the microarray over 100 times for glycan profiling using the model protein Prostate Specific Antigen (PSA). The glycan profile of PSA enriched from LNCAP cells, obtained at a sub-nanogram level with the aid of TSA, was consistent with the previous reports. We also established the glycan profile of Prostate Specific Membrane Antigen (PSMA) using the TSA and ALM. Thus, the Tyramide Signal Amplification for Antibody-overlay Lectin Microarray is a sensitive, rapid, comprehensive, and high-throughput method for targeted glycan profiling and can potentially be used for the identification of disease-related protein glycoforms. PMID:21133419

  15. Temporal and spatial profiling of nuclei-associated proteins upon TNF-α/NF-κB signaling

    Institute of Scientific and Technical Information of China (English)

    Danjun Ma; SuJun Li; LianShui Wang; Jie Dai; Shilin Zhao; Rong Zeng

    2009-01-01

    The tumor necrosis factor (TNF)-α/NF-κB-signaling pathway plays a pivotal role in various processes including apoptosis, cellular differentiation, host defense, inflammation, autoimmunity and organogenesis. The complexity of the TNF-α/NF-κB signaling is in part due to the dynamic protein behaviors of key players in this pathway, in this present work, a dynamic and global view of the signaling components in the nucleus at the early stages of TNF-α/ NF-κB signaling was obtained in HEK293 cells, by a combination of subcellular fractionation and stable isotope la-beling by amino acids in cell culture (SILAC). The dynamic profile patterns of 547 TNF-α-induced nuclei-associated proteins were quantified in our studies. The functional characters of all the profiles were further analyzed using that Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation. Additionally, many previously unknown effectors of TNF-α/NF-κB signaling were identified, quantified and clustered into differential activation profiles. In-terestingly, levels of Fanconi anemia group D2 protein (FANCD2), one of the Fanconi anemia family proteins, was found to be increased in the nucleus by SILAC quantitation upon TNF-α stimulation, which was further verified by western blotting and immunofluorescence analysis. This indicates that FANCD2 might be involved in TNF-α/NF-κB signaling through its accumulation in the nucleus, in summary, the combination of subcellular proteomics with quan-titative analysis not only allowed for a dissection of the nuclear TNF-α/NF-κB-signaling pathway, but also provided a systematic strategy for monitoring temporal and spatial changes in cell signaling.

  16. Fast estimation of defect profiles from the magnetic flux leakage signal based on a multi-power affine projection algorithm.

    Science.gov (United States)

    Han, Wenhua; Shen, Xiaohui; Xu, Jun; Wang, Ping; Tian, Guiyun; Wu, Zhengyang

    2014-09-04

    Magnetic flux leakage (MFL) inspection is one of the most important and sensitive nondestructive testing approaches. For online MFL inspection of a long-range railway track or oil pipeline, a fast and effective defect profile estimating method based on a multi-power affine projection algorithm (MAPA) is proposed, where the depth of a sampling point is related with not only the MFL signals before it, but also the ones after it, and all of the sampling points related to one point appear as serials or multi-power. Defect profile estimation has two steps: regulating a weight vector in an MAPA filter and estimating a defect profile with the MAPA filter. Both simulation and experimental data are used to test the performance of the proposed method. The results demonstrate that the proposed method exhibits high speed while maintaining the estimated profiles clearly close to the desired ones in a noisy environment, thereby meeting the demand of accurate online inspection.

  17. An astrometric search for a stellar companion to the sun

    Energy Technology Data Exchange (ETDEWEB)

    Perlmutter, S.

    1986-11-25

    A companion star within 0.8 pc of the Sun has been postulated to explain a possible 26 Myr periodicity in mass extinctions of species on the Earth. Such a star would already be catalogued in the Yale Bright Star catalogue unless it is fainter than m/sub nu/ = 6.5; this limits the possible stellar types for an unseen companion to red dwarfs, brown dwarfs, or compact objects. Red dwarfs account for about 75% of these possible stars. We describe here the design and development of an astrometric search for a nearby red dwarf companion with a six-month peak-to-peak parallax of greater than or equal to2.5 arcseconds. We are measuring the parallax of 2770 candidate faint red stars selected from the Dearborn Observatory catalogue. An automated 30-inch telescope and CCD camera system collect digitized images of the candidate stars, along with a 13' x 16' surrounding field of background stars. Second-epoch images, taken a few months later, are registered to the first epoch images using the background stars as fiducials. An apparent motion, m/sub a/, of the candidate stars is found to a precision of sigma/sub m//sub a/ approx. = 0.08 pixel approx. = 0.2 arcseconds for fields with N/sub fiducial/ greater than or equal to 10 fiducial stars visible above the background noise. This precision is sufficient to detect the parallactic motion of a star at 0.8 pc with a two month interval between the observation epochs. Images with fewer fiducial stars above background noise are observed with a longer interval between epochs. If a star is found with high parallactic motion, we will confirm its distance with further parallax measurements, photometry, and spectral studies, and will measure radial velocity and proper motion to establish its orbit. We have demonstrated the search procedure with observations of 41 stars, and have shown that none of these is a nearby star. 37 refs., 16 figs., 3 tabs.

  18. Molecular profiling reveals diversity of stress signal transduction cascades in highly penetrant Alzheimer's disease human skin fibroblasts.

    Directory of Open Access Journals (Sweden)

    Graziella Mendonsa

    Full Text Available The serious and growing impact of the neurodegenerative disorder Alzheimer's disease (AD as an individual and societal burden raises a number of key questions: Can a blanket test for Alzheimer's disease be devised forecasting long-term risk for acquiring this disorder? Can a unified therapy be devised to forestall the development of AD as well as improve the lot of present sufferers? Inflammatory and oxidative stresses are associated with enhanced risk for AD. Can an AD molecular signature be identified in signaling pathways for communication within and among cells during inflammatory and oxidative stress, suggesting possible biomarkers and therapeutic avenues? We postulated a unique molecular signature of dysfunctional activity profiles in AD-relevant signaling pathways in peripheral tissues, based on a gain of function in G-protein-coupled bradykinin B2 receptor (BKB2R inflammatory stress signaling in skin fibroblasts from AD patients that results in tau protein Ser hyperphosphorylation. Such a signaling profile, routed through both phosphorylation and proteolytic cascades activated by inflammatory and oxidative stresses in highly penetrant familial monogenic forms of AD, could be informative for pathogenesis of the complex multigenic sporadic form of AD. Comparing stimulus-specific cascades of signal transduction revealed a striking diversity of molecular signaling profiles in AD human skin fibroblasts that express endogenous levels of mutant presenilins PS-1 or PS-2 or the Trisomy 21 proteome. AD fibroblasts bearing the PS-1 M146L mutation associated with highly aggressive AD displayed persistent BKB2R signaling plus decreased ERK activation by BK, correctible by gamma-secretase inhibitor Compound E. Lack of these effects in the homologous PS-2 mutant cells indicates specificity of presenilin gamma-secretase catalytic components in BK signaling biology directed toward MAPK activation. Oxidative stress revealed a JNK-dependent survival

  19. The Full-sky Astrometric Mapping Explorer - Astrometry for the New Millennium

    Science.gov (United States)

    Horner, S. D.; Germain, M. E.; Greene, T. P.; Harris, F. H.; Johnson, M. S.; Johnson, K. J.; Monet, D. G.; Murison, M. A.; Phillips, J. D.; Reasenberg, R. D.; Seidelmann, P. K.; Urban, S. E.; Vassar, R. H.

    FAME is designed to perform an all-sky, astrometric survey with unprecedented accuracy. It will create a rigid astrometric catalog of ~40,000,000 stars with visual band magnitudes 5 DSS colors. The FAME data will provide a rigid, accurate, optical, astrometric grid. The proper motion data, combined with Hipparcos and other data should be ideal for use by the Space Interferometry Mission (SIM) to select its astrometric reference grid stars. FAME will also identify stars with nonlinear proper motions as candidates for further study by SIM, Terrestrial Planet Finder, and future ground based interferometers as possible planetary systems. The fundamental astrometric data provided at relatively low cost by FAME will help optimize the scientific return from these future projects. This is in addition to the considerable direct scientific return from FAME. It will redefine the extragalactic distance scale and provide a large, rich database of information on stellar properties that will enable numerous science investigations into stellar structure and evolution, the dynamics of the Milky Way, and stellar companions including brown dwarfs and giant planets. NASA has selected the Full-sky Astrometric Mapping Explorer (FAME) to be one of five MIDEX missions to be funded for a concept study. This concept study will be submitted to NASA on 18 June, with final selection, scheduled for September, of two of these missions for fli ght in 2003 or 2004. FAME is a joint development e ffort of the U.S. Naval Observatory, the Smithsonian Astrophysical Observatory, the Infrared Processing and Analysis Center, Lockheed Martin Missiles and Space, the Naval Research Laboratory, and Omitron Incorporated.

  20. Spatially Resolved Genome-wide Transcriptional Profiling Identifies BMP Signaling as Essential Regulator of Zebrafish Cardiomyocyte Regeneration.

    Science.gov (United States)

    Wu, Chi-Chung; Kruse, Fabian; Vasudevarao, Mohankrishna Dalvoy; Junker, Jan Philipp; Zebrowski, David C; Fischer, Kristin; Noël, Emily S; Grün, Dominic; Berezikov, Eugene; Engel, Felix B; van Oudenaarden, Alexander; Weidinger, Gilbert; Bakkers, Jeroen

    2016-01-11

    In contrast to mammals, zebrafish regenerate heart injuries via proliferation of cardiomyocytes located near the wound border. To identify regulators of cardiomyocyte proliferation, we used spatially resolved RNA sequencing (tomo-seq) and generated a high-resolution genome-wide atlas of gene expression in the regenerating zebrafish heart. Interestingly, we identified two wound border zones with distinct expression profiles, including the re-expression of embryonic cardiac genes and targets of bone morphogenetic protein (BMP) signaling. Endogenous BMP signaling has been reported to be detrimental to mammalian cardiac repair. In contrast, we find that genetic or chemical inhibition of BMP signaling in zebrafish reduces cardiomyocyte dedifferentiation and proliferation, ultimately compromising myocardial regeneration, while bmp2b overexpression is sufficient to enhance it. Our results provide a resource for further studies on the molecular regulation of cardiac regeneration and reveal intriguing differential cellular responses of cardiomyocytes to a conserved signaling pathway in regenerative versus non-regenerative hearts.

  1. Statistical analysis of the astrometric errors for the most productive asteroid surveys

    Science.gov (United States)

    Vereš, Peter; Farnocchia, Davide; Chesley, Steven R.; Chamberlin, Alan

    2016-10-01

    Accurate orbits of minor planets allow reliable predictions of an object's location in time and space. High fidelity ephemerides are crucial for the space missions targeting asteroids and comets, mitigation of Earth impact hazard, study of non-gravitational effects on small bodies and mass determination of encountering objects through mutual perturbations. The length of the observation arc as well as high quality astrometry play an essential role in achieving accurate orbits. In particular, accurate astrometry can allow the recovery of small near-Earth objects that could otherwise be lost. The vast majority of the 715,000 known asteroids have been discovered and observed by major dedicated optical CCD surveys. However, uncertainties of individual astrometric positions are not directly provided by observers yet and so orbit determination traditionally relies on conservative estimates of astrometric errors. We present a statistical study of astrometric residuals of optical CCD astrometry for the nine most prolific past and current asteroid surveys: Pan-STARRS1 (F51), Mt. Lemmon (G96), Catalina (703), LINEAR (704), Spacewatch (691), LONEOS (699), NEAT (644), NEOWISE (C51) and SST (G45). The study was limited to multiple apparition asteroids, which have well-constrained orbits, after correcting for the star catalog position and proper motion biases (Farnocchia et al., 2015). Therefore, the resulting astrometric residuals can be largely attributed to astrometric and timing errors in the reported astrometry. We analyze the behavior of residuals in right ascension, declination, along-track and cross-track, as well as timing errors. Astrometric residuals generally depend on reported magnitude by a quadratic function with astrometric quality degradation near the limiting magnitude and the saturation limit for bright objects. We found no systematic timing errors exceeding one second for the tested surveys. The presented analysis provides useful information to improve the

  2. Developmental Profile and Mechanisms of GABA-Induced Calcium Signaling in Hippocampal Astrocytes

    Institute of Scientific and Technical Information of China (English)

    SILKE D. MEIER; KARL W. KAFITZ; CHRISTINE R. ROSE

    2008-01-01

    γ-氨基丁酸(GABA)是具有双重作用的递质,它在产后发育的第1周对神经元具有兴奋作用,但在成年大脑中是主要的抑制性递质.GABA还能通过与离子型(GABAA)和代谢型(GABAB)受体结合来活化星形胶质细胞,导致胶质细胞钙升高及神经递质释放,GABA在神经元-胶质细胞相互作用中起重要的调节作用.本文采用全细胞膜片钳和比率钙成象分析出生后3~34 d的大鼠海马切片,星形胶质细胞GABAA和GABAB受体活化诱导的钙信号的发育特征及细胞机制.GABAA和GABAB受体都可介导胶质细胞的细胞内钙瞬对升高.在整个发育过程中,GABAA受体活化通过激活电压依赖性钙通道的钙流入引起大多数星形胶质细胞快速的钙瞬变.相反的是,GABAB受体活化导致细胞延迟的钙升高,并且这种作用能被细胞内钙库消耗和持久的异源三聚G蛋白活化所阻滞.GABAB受体介导的钙信号呈现明确的发育规律,即<10%的星形胶质细胞在出生后3 d或32~34 d有应答,大约60%的星形胶质细胞在出生后11~15 d有应答.本文提示,GABAB受体通过激活G蛋白,诱导细胞内钙库释放钙,导致细胞的钙瞬变.星形胶质细胞中GABAB受体介导的钙信号在出生后海马网络发育完成时优先出现.%GABA (γ-aminobutyric acid) is a transmitter with dual action. Whereas it excites neurons during the first week of postnatal development, it represents the major inhibitory transmitter in the mature brain. GABA also activates astrocytes by binding to ionotropic (GABAA) and metabotropic (GABAB) receptors. This results in glial calcium transients which can induce the release of gliotransmitters, rendering GABA an important mediator of neuron-glia interaction. Using whole-cell patch-clamp and ratiometric calcium imaging in hippocampal slices from rats at postnatal days 3~34, we have analyzed the developmental profile as well as the cellular mechanisms of calcium signals induced by

  3. Analyzing the characteristics of phase-shift keyed signals applied to the measurement of an electron concentration profile using the radiophysical model of the ionosphere

    Science.gov (United States)

    Alsatkin, S. S.; Medvedev, A. V.; Kushnarev, D. S.

    2009-12-01

    The radiophysical model of the ionosphere, which can approximately imitate the ionospheric response to a sounding signal, is presented. The model mathematical description and input parameters are given. The results of modeling the ionospheric response to different types of sounding signals are presented. The methods for estimating the quality of restoration of an initial power profile are described. The optimal characteristics of the signal, which allows for a high-quality restoration of an initial power profile, and the modeling results are presented.

  4. Systematic profiling of Caenorhabditis elegans locomotive behaviors reveals additional components in G-protein Gαq signaling.

    Science.gov (United States)

    Yu, Hui; Aleman-Meza, Boanerges; Gharib, Shahla; Labocha, Marta K; Cronin, Christopher J; Sternberg, Paul W; Zhong, Weiwei

    2013-07-16

    Genetic screens have been widely applied to uncover genetic mechanisms of movement disorders. However, most screens rely on human observations of qualitative differences. Here we demonstrate the application of an automatic imaging system to conduct a quantitative screen for genes regulating the locomotive behavior in Caenorhabditis elegans. Two hundred twenty-seven neuronal signaling genes with viable homozygous mutants were selected for this study. We tracked and recorded each animal for 4 min and analyzed over 4,400 animals of 239 genotypes to obtain a quantitative, 10-parameter behavioral profile for each genotype. We discovered 87 genes whose inactivation causes movement defects, including 50 genes that had never been associated with locomotive defects. Computational analysis of the high-content behavioral profiles predicted 370 genetic interactions among these genes. Network partition revealed several functional modules regulating locomotive behaviors, including sensory genes that detect environmental conditions, genes that function in multiple types of excitable cells, and genes in the signaling pathway of the G protein Gαq, a protein that is essential for animal life and behavior. We developed quantitative epistasis analysis methods to analyze the locomotive profiles and validated the prediction of the γ isoform of phospholipase C as a component in the Gαq pathway. These results provided a system-level understanding of how neuronal signaling genes coordinate locomotive behaviors. This study also demonstrated the power of quantitative approaches in genetic studies.

  5. Transcriptional profiling of ErbB signalling in mammary luminal epithelial cells - interplay of ErbB and IGF1 signalling through IGFBP3 regulation

    Directory of Open Access Journals (Sweden)

    Worthington Jenny

    2010-09-01

    Full Text Available Abstract Background Members of the ErbB family of growth factor receptors are intricately linked with epithelial cell biology, development and tumourigenesis; however, the mechanisms involved in their downstream signalling are poorly understood. Indeed, it is unclear how signal specificity is achieved and the relative contribution each receptor has to specific gene expression. Methods Gene expression profiling of a human mammary luminal epithelial cell model of ErbB2-overexpression was carried out using cDNA microarrays with a common RNA reference approach to examine long-term overlapping and differential responses to EGF and heregulin beta1 treatment in the context of ErbB2 overexpression. Altered gene expression was validated using quantitative real time PCR and/or immunoblotting. One gene of interest was targeted for further characterisation, where the effects of siRNA-mediated silencing on IGF1-dependent signalling and cellular phenotype were examined and compared to the effects of loss of ErbB2 expression. Results 775 genes were differentially expressed and clustered in terms of their growth factor responsiveness. As well as identifying uncharacterized genes as novel targets of ErbB2-dependent signalling, ErbB2 overexpression augmented the induction of multiple genes involved in proliferation (e.g. MYC, MAP2K1, MAP2K3, autocrine growth factor signalling (VEGF, PDGF and adhesion/cytoskeletal regulation (ZYX, THBS1, VCL, CNN3, ITGA2, ITGA3, NEDD9, TAGLN, linking them to the hyper-poliferative and altered adhesive phenotype of the ErbB2-overexpressing cells. We also report ErbB2-dependent down-regulation of multiple interferon-stimulated genes that may permit ErbB2-overexpressing cells to resist the anti-proliferative action of interferons. Finally, IGFBP3 was unique in its pattern of regulation and we further investigated a possible role for IGFBP3 down-regulation in ErbB2-dependent transformation through suppressed IGF1 signalling. We show

  6. The resolutions of the 24th IAU General Assembly and presedent astrometric research fields

    Science.gov (United States)

    Jin, Wenjing; Xia, Yifei; Han, Changhao

    2001-06-01

    The action of IAU resolutions for the development of astronomy are described and the three resolutions, which were passed at the 24th IAU General Assembly, are briefly introduced. The precedent astrometric research fields during the period of 2000 - 2003 are evaluated and the prior research fields of fundamental astrometry in China are suggested.

  7. The astrometric Gaia-FUN-SSO observation campaign of 99 942 Apophis

    CERN Document Server

    Thuillot, W; Ivantsov, A; Desmars, J; Assafin, M; Eggl, S; Hestroffer, D; Rocher, P; Carry, B; David, P; Abe, L; Andreev, M; Arlot, J -E; Asami, A; Ayvasian, V; Baransky, A; Belcheva, M; Bendjoya, Ph; Bikmaev, I; Burkhonov, O A; Camci, U; Carbognani, A; Colas, F; Devyatkin, A V; Ehgamberdiev, Sh A; Enikova, P; Eyer, L; Galeev, A; Gerlach, E; Godunova, V; Golubaev, A V; Gorshanov, D L; Gumerov, R; Hashimoto, N; Helvaci, M; Ibryamov, S; Inasaridze, R Ya; Khamitov, I; Kostov, A; Kozhukhov, A M; Kozyryev, Y; Krugly, Yu N; Kryuchkovskiy, V; Kulichenko, N; Maigurova, N; Manilla-Robles, A; Martyusheva, A A; Molotov, I E; Nikolov, G; Nikolov, P; Nishiyama, K; Okumura, S; Palaversa, L; Parmonov, O; Peng, Q Y; Petrova, S N; Pinigin, G I; Pomazan, A; Rivet, J -P; Sakamoto, T; Sakhibullin, N; Sergeev, O; Sergeyev, A V; Shulga, O V; Suarez, O; Sybiryakova, Y; Takahashi, N; Tarady, V; Todd, M; Urakawa, S; Uysal, O; Vaduvescu, O; Vovk, V; Zhang, X -L

    2015-01-01

    Astrometric observations performed by the Gaia Follow-Up Network for Solar System Objects (Gaia-FUN-SSO) play a key role in ensuring that moving objects first detected by ESA's Gaia mission remain recoverable after their discovery. An observation campaign on the potentially hazardous asteroid (99 942) Apophis was conducted during the asteroid's latest period of visibility, from 12/21/2012 to 5/2/2013, to test the coordination and evaluate the overall performance of the Gaia-FUN-SSO . The 2732 high quality astrometric observations acquired during the Gaia-FUN-SSO campaign were reduced with the Platform for Reduction of Astronomical Images Automatically (PRAIA), using the USNO CCD Astrograph Catalogue 4 (UCAC4) as a reference. The astrometric reduction process and the precision of the newly obtained measurements are discussed. We compare the residuals of astrometric observations that we obtained using this reduction process to data sets that were individually reduced by observers and accepted by the Minor Plane...

  8. Determination of the small Solar system bodies orbital elements from astrometric observations with OMT-800 telescope

    Science.gov (United States)

    Troianskyi, Volodymyr; Bazey, A. A.; Kashuba, V. I.; Zhukov, V. V.

    2014-11-01

    From the beginning of operation of the new OMT-800 telescope in late 2012 we were able to recieve the high-precision differential astrometrical observations of geostationary objects, asteroids and comets brighter than 21 mag. In this work, the technique of calculation of the orbital elements and prediction of the geostationary objects and asteroids trajectory are considered

  9. Astrometric techniques; Proceedings of the Symposium, Gainesville, FL, January 9-12, 1984

    Science.gov (United States)

    Eichhorn, H. K.; Leacock, R. J.

    The conference presents papers on the reduction technique, radio astrometry, photographic astrometry, interferometry, small field photoelectric astronomy, transit circles and astrolabes, space astronomy, objects, administration and distribution, and connections between the various techniques. Particular attention is given to the concepts of reference systems, the relativistic reduction of astrometric observations, computation of compilation catalogs, global reduction of fundamental astrometric data, the astrometric possibilities of very-long-base interferometry and the JPL/DSN J2000 radio reference frame. Papers are also presented on proper motions with respect to galaxies, plans for the second epoch of the southern proper motion program, trigonometric parallaxes obtained with the UK Schmidt telescope, astrometry with the Lowell PDS, speckle interferometry in astrometry, moving-image astrometry with the multianode microchannel array, timing and data acquisition for a field astrolabe, the use of photographic positions in determining the azimuth of a meridian circle, the Hipparcos satellite and the organization of the project, astrometric desiderata for nearby stars, astrometry in China, and the need for better cooperation and intercomparison in fundamental astrometry.

  10. Gravitational lensing statistics with extragalactic surveys; 2, Analysis of the Jodrell Bank-VLA Astrometric Survey

    NARCIS (Netherlands)

    Helbig, P.; Marlow, D. R.; Quast, R.; Wilkinson, P. N.; Browne, I. W. A.; Koopmans, L. V. E.

    1999-01-01

    Published in: Astron. Astrophys. Suppl. Ser. 136 (1999) no. 2, pp.297-305 citations recorded in [Science Citation Index] Abstract: We present constraints on the cosmological constant $lambda_{0}$ from gravitational lensing statistics of the Jodrell Bank-VLA Astrometric Survey (JVAS). Although this

  11. Gravitational lensing statistics with extragalactic surveys - II. Analysis of the Jodrell Bank-VLA Astrometric Survey

    NARCIS (Netherlands)

    Helbig, P; Marlow, D; Quast, R; Wilkinson, PN; Browne, IWA; Koopmans, LVE

    We present constraints on the cosmological constant lambda(0) from gravitational lensing statistics of the Jodrell Bank-VLA Astrometric Survey (JVAS). Although this is the largest gravitational lens survey which has been analysed, cosmological constraints are only comparable to those from optical

  12. Profiling 976 ToxCast chemicals across 331 enzymatic and receptor signaling assays (Communities of Practice)

    Science.gov (United States)

    Understanding potential health risks is a significant challenge for large numbers of diverse chemicals with poorly characterized exposures and mechanisms of toxicities. The present study analyzes chemical-target activity profiles of 976 chemicals (including failed pharmaceuticals...

  13. Molecular pathway profiling of T lymphocyte signal transduction pathways; Th1 and Th2 genomic fingerprints are defined by TCR and CD28-mediated signaling

    Directory of Open Access Journals (Sweden)

    Smeets Ruben L

    2012-03-01

    Full Text Available Abstract Background T lymphocytes are orchestrators of adaptive immunity. Naïve T cells may differentiate into Th1, Th2, Th17 or iTreg phenotypes, depending on environmental co-stimulatory signals. To identify genes and pathways involved in differentiation of Jurkat T cells towards Th1 and Th2 subtypes we performed comprehensive transcriptome analyses of Jurkat T cells stimulated with various stimuli and pathway inhibitors. Results from these experiments were validated in a human experimental setting using whole blood and purified CD4+ Tcells. Results Calcium-dependent activation of T cells using CD3/CD28 and PMA/CD3 stimulation induced a Th1 expression profile reflected by increased expression of T-bet, RUNX3, IL-2, and IFNγ, whereas calcium-independent activation via PMA/CD28 induced a Th2 expression profile which included GATA3, RXRA, CCL1 and Itk. Knock down with siRNA and gene expression profiling in the presence of selective kinase inhibitors showed that proximal kinases Lck and PKCθ are crucial signaling hubs during T helper cell activation, revealing a clear role for Lck in Th1 development and for PKCθ in both Th1 and Th2 development. Medial signaling via MAPkinases appeared to be less important in these pathways, since specific inhibitors of these kinases displayed a minor effect on gene expression. Translation towards a primary, whole blood setting and purified human CD4+ T cells revealed that PMA/CD3 stimulation induced a more pronounced Th1 specific, Lck and PKCθ dependent IFNγ production, whereas PMA/CD28 induced Th2 specific IL-5 and IL-13 production, independent of Lck activation. PMA/CD3-mediated skewing towards a Th1 phenotype was also reflected in mRNA expression of the master transcription factor Tbet, whereas PMA/CD28-mediated stimulation enhanced GATA3 mRNA expression in primary human CD4+ Tcells. Conclusions This study identifies stimulatory pathways and gene expression profiles for in vitro skewing of T helper cell

  14. Application of Finite Difference Technique to Raman Lidar Signals to Derive the Altitude Profiles of Atmospheric Aerosol Extinction

    Directory of Open Access Journals (Sweden)

    PURUSOTHAM S

    2015-06-01

    Full Text Available Lidars (Laser radars are the best suitable instruments to derive the range resolved parameters of atmosphere. Single wavelength and simple backscatter lidars have been widely used to study the height profiles of particle scattering and extinction in the atmosphere. However, atmospheric extinction derived using these lidars data undergo several assumptions and hence involve a significant amount of error in estimation of extinction. The Raman lidar methodology of deriving particle extinction in the atmosphere is a simplified straight-forward method that does not involve any assumptions. The Raman lidar method of atmospheric extinction computation employs derivative of logarithm of normalized range corrected Raman backscattered signal. Usually this causes gaps in the height profiles wherever there is a gradient in the signal under examination. In the present study, a new method is proposed to derive the particle extinction in the atmospheric boundary layer. In this new method, a scheme of alternative solution methodology has been proposed using “Finite Difference Technique”. The method has an advantage that, it does not involve the gradient as compared to conventional technique and hence reduces the error. Using this method, the height profiles of particle extinction has been derived. A code in MATLAB is developed to derive the altitude distribution of aerosol extinction. In this connection, the NOAA-REDY site data has been used as the reference data for calculating the molecular extinction in the lower atmosphere.

  15. Large-scale profiling of signalling pathways reveals an asthma specific signature in bronchial smooth muscle cells

    Science.gov (United States)

    Alexandrova, Elena; Nassa, Giovanni; Corleone, Giacomo; Buzdin, Anton; Aliper, Alexander M.; Terekhanova, Nadezhda; Shepelin, Denis; Zhavoronkov, Alexander; Tamm, Michael; Milanesi, Luciano; Weisz, Alessandro

    2016-01-01

    Background Bronchial smooth muscle (BSM) cells from asthmatic patients maintain in vitro a distinct hyper-reactive (“primed”) phenotype, characterized by increased release of pro-inflammatory factors and mediators, as well as hyperplasia and/or hypertrophy. This “primed” phenotype helps to understand pathogenesis of asthma, as changes in BSM function are essential for manifestation of allergic and inflammatory responses and airway wall remodelling. Objective To identify signalling pathways in cultured primary BSMs of asthma patients and non-asthmatic subjects by genome wide profiling of differentially expressed mRNAs and activated intracellular signalling pathways (ISPs). Methods Transcriptome profiling by cap-analysis-of-gene-expression (CAGE), which permits selection of preferentially capped mRNAs most likely to be translated into proteins, was performed in human BSM cells from asthmatic (n=8) and non-asthmatic (n=6) subjects and OncoFinder tool were then exploited for identification of ISP deregulations. Results CAGE revealed >600 RNAs differentially expressed in asthma vs control cells (p≤0.005), with asthma samples showing a high degree of similarity among them. Comprehensive ISP activation analysis revealed that among 269 pathways analysed, 145 (ppromoting pathways and up-regulated ones affecting cell growth and proliferation, inflammatory response, control of smooth muscle contraction and hypoxia-related signalization. Conclusions These first-time results can now be exploited toward development of novel therapeutic strategies targeting ISP signatures linked to asthma pathophysiology. PMID:26863634

  16. Profiling small RNA reveals multimodal substructural signals in a Boltzmann ensemble

    Science.gov (United States)

    Rogers, Emily; Heitsch, Christine E.

    2014-01-01

    As the biomedical impact of small RNAs grows, so does the need to understand competing structural alternatives for regions of functional interest. Suboptimal structure analysis provides significantly more RNA base pairing information than a single minimum free energy prediction. Yet computational enhancements like Boltzmann sampling have not been fully adopted by experimentalists since identifying meaningful patterns in this data can be challenging. Profiling is a novel approach to mining RNA suboptimal structure data which makes the power of ensemble-based analysis accessible in a stable and reliable way. Balancing abstraction and specificity, profiling identifies significant combinations of base pairs which dominate low-energy RNA secondary structures. By design, critical similarities and differences are highlighted, yielding crucial information for molecular biologists. The code is freely available via http://gtfold.sourceforge.net/profiling.html. PMID:25392423

  17. Expression profile of critical genes involved in FGF signaling pathway in the developing human primary dentition.

    Science.gov (United States)

    Huang, Feng; Hu, Xiaoxiao; Fang, Chunni; Liu, Hong; Lin, Chensheng; Zhang, Yanding; Hu, Xuefeng

    2015-11-01

    Mammalian tooth development is regulated by paracrine signal molecules of several conserved family interactions between epithelium and mesenchyme. The expression patterns and regulative roles of FGF signaling have been extensively studied in the mouse odontogenesis; however, that is not well known in human tooth development. In order to unveil the molecular mechanisms that regulate human tooth morphogenesis, we examined the expression patterns of the critical molecules involved in FGF signaling pathway in the developing human tooth germ by in situ hybridization, immunohistochemistry, and real-time RT-PCR, including FGF ligands, receptors, and intracellular transducer. We found overlapping but distinct expression pattern of FGF ligands and receptors in the different stages and components. Expression of FGF4, FGF7, FGF8, and FGF9 persists widespread in human tooth mesenchyme, which is quite different to that of in mouse. FGFR1 may be the major receptor in regulate mechanisms of FGF signals in human tooth development. Real-time RT-PCR indeed confirmed the results of in situ hybridization. Results of K-Ras, p-ERK1/2, p-p38, p-JNK, and p-PDK1 expression reveal spatial and temporal patterns of FGF signaling during morphogenesis and organogenesis of human tooth germ. Activity of the FGF signaling transducer protein in human tooth germ was much higher than that of in mouse. Our results provided important FGF singling information in the developing process, pinpoint to the domains where the downstream target genes of FGF signaling can be sought, and enlightened our knowledge about the nature of FGF signaling in human tooth germ.

  18. Daily genetic profiling indicates JAK/STAT signaling promotes early hepatic stellate cell transdifferentiation

    Institute of Scientific and Technical Information of China (English)

    Ashley; M; Lakner; Cathy; C; Moore; Alyssa; A; Gulledge; Laura; W; Schrum

    2010-01-01

    AIM: To identify signaling pathways and genes that initiate and commit hepatic stellate cells (HSCs) to transdifferentiation. METHODS: Primary HSCs were isolated from male Sprague-Dawley rats and cultured on plastic for 0-10 d. Gene expression was assessed daily (quiescent to day 10 culture-activation) by real time polymerase chain reaction and data clustered using AMADA software. The significance of JAK/STAT signaling to HSC transdifferentiation was determined by treating cells with a JAK2 inhibitor. RESUL...

  19. Prediction of Clinically Relevant Safety Signals of Nephrotoxicity through Plasma Metabolite Profiling

    Directory of Open Access Journals (Sweden)

    W. B. Mattes

    2013-01-01

    Full Text Available Addressing safety concerns such as drug-induced kidney injury (DIKI early in the drug pharmaceutical development process ensures both patient safety and efficient clinical development. We describe a unique adjunct to standard safety assessment wherein the metabolite profile of treated animals is compared with the MetaMap Tox metabolomics database in order to predict the potential for a wide variety of adverse events, including DIKI. To examine this approach, a study of five compounds (phenytoin, cyclosporin A, doxorubicin, captopril, and lisinopril was initiated by the Technology Evaluation Consortium under the auspices of the Drug Safety Executive Council (DSEC. The metabolite profiles for rats treated with these compounds matched established reference patterns in the MetaMap Tox metabolomics database indicative of each compound’s well-described clinical toxicities. For example, the DIKI associated with cyclosporine A and doxorubicin was correctly predicted by metabolite profiling, while no evidence for DIKI was found for phenytoin, consistent with its clinical picture. In some cases the clinical toxicity (hepatotoxicity, not generally seen in animal studies, was detected with MetaMap Tox. Thus metabolite profiling coupled with the MetaMap Tox metabolomics database offers a unique and powerful approach for augmenting safety assessment and avoiding clinical adverse events such as DIKI.

  20. Gaia Data Release 1. Summary of the astrometric, photometric, and survey properties

    Science.gov (United States)

    Gaia Collaboration; Brown, A. G. A.; Vallenari, A.; Prusti, T.; de Bruijne, J. H. J.; Mignard, F.; Drimmel, R.; Babusiaux, C.; Bailer-Jones, C. A. L.; Bastian, U.; Biermann, M.; Evans, D. W.; Eyer, L.; Jansen, F.; Jordi, C.; Katz, D.; Klioner, S. A.; Lammers, U.; Lindegren, L.; Luri, X.; O'Mullane, W.; Panem, C.; Pourbaix, D.; Randich, S.; Sartoretti, P.; Siddiqui, H. I.; Soubiran, C.; Valette, V.; van Leeuwen, F.; Walton, N. A.; Aerts, C.; Arenou, F.; Cropper, M.; Høg, E.; Lattanzi, M. G.; Grebel, E. K.; Holland, A. D.; Huc, C.; Passot, X.; Perryman, M.; Bramante, L.; Cacciari, C.; Castañeda, J.; Chaoul, L.; Cheek, N.; De Angeli, F.; Fabricius, C.; Guerra, R.; Hernández, J.; Jean-Antoine-Piccolo, A.; Masana, E.; Messineo, R.; Mowlavi, N.; Nienartowicz, K.; Ordóñez-Blanco, D.; Panuzzo, P.; Portell, J.; Richards, P. J.; Riello, M.; Seabroke, G. M.; Tanga, P.; Thévenin, F.; Torra, J.; Els, S. G.; Gracia-Abril, G.; Comoretto, G.; Garcia-Reinaldos, M.; Lock, T.; Mercier, E.; Altmann, M.; Andrae, R.; Astraatmadja, T. L.; Bellas-Velidis, I.; Benson, K.; Berthier, J.; Blomme, R.; Busso, G.; Carry, B.; Cellino, A.; Clementini, G.; Cowell, S.; Creevey, O.; Cuypers, J.; Davidson, M.; De Ridder, J.; de Torres, A.; Delchambre, L.; Dell'Oro, A.; Ducourant, C.; Frémat, Y.; García-Torres, M.; Gosset, E.; Halbwachs, J.-L.; Hambly, N. C.; Harrison, D. L.; Hauser, M.; Hestroffer, D.; Hodgkin, S. T.; Huckle, H. E.; Hutton, A.; Jasniewicz, G.; Jordan, S.; Kontizas, M.; Korn, A. J.; Lanzafame, A. C.; Manteiga, M.; Moitinho, A.; Muinonen, K.; Osinde, J.; Pancino, E.; Pauwels, T.; Petit, J.-M.; Recio-Blanco, A.; Robin, A. C.; Sarro, L. M.; Siopis, C.; Smith, M.; Smith, K. W.; Sozzetti, A.; Thuillot, W.; van Reeven, W.; Viala, Y.; Abbas, U.; Abreu Aramburu, A.; Accart, S.; Aguado, J. J.; Allan, P. M.; Allasia, W.; Altavilla, G.; Álvarez, M. A.; Alves, J.; Anderson, R. I.; Andrei, A. H.; Anglada Varela, E.; Antiche, E.; Antoja, T.; Antón, S.; Arcay, B.; Bach, N.; Baker, S. G.; Balaguer-Núñez, L.; Barache, C.; Barata, C.; Barbier, A.; Barblan, F.; Barrado y Navascués, D.; Barros, M.; Barstow, M. A.; Becciani, U.; Bellazzini, M.; Bello García, A.; Belokurov, V.; Bendjoya, P.; Berihuete, A.; Bianchi, L.; Bienaymé, O.; Billebaud, F.; Blagorodnova, N.; Blanco-Cuaresma, S.; Boch, T.; Bombrun, A.; Borrachero, R.; Bouquillon, S.; Bourda, G.; Bouy, H.; Bragaglia, A.; Breddels, M. A.; Brouillet, N.; Brüsemeister, T.; Bucciarelli, B.; Burgess, P.; Burgon, R.; Burlacu, A.; Busonero, D.; Buzzi, R.; Caffau, E.; Cambras, J.; Campbell, H.; Cancelliere, R.; Cantat-Gaudin, T.; Carlucci, T.; Carrasco, J. M.; Castellani, M.; Charlot, P.; Charnas, J.; Chiavassa, A.; Clotet, M.; Cocozza, G.; Collins, R. S.; Costigan, G.; Crifo, F.; Cross, N. J. G.; Crosta, M.; Crowley, C.; Dafonte, C.; Damerdji, Y.; Dapergolas, A.; David, P.; David, M.; De Cat, P.; de Felice, F.; de Laverny, P.; De Luise, F.; De March, R.; de Martino, D.; de Souza, R.; Debosscher, J.; del Pozo, E.; Delbo, M.; Delgado, A.; Delgado, H. E.; Di Matteo, P.; Diakite, S.; Distefano, E.; Dolding, C.; Dos Anjos, S.; Drazinos, P.; Duran, J.; Dzigan, Y.; Edvardsson, B.; Enke, H.; Evans, N. W.; Eynard Bontemps, G.; Fabre, C.; Fabrizio, M.; Faigler, S.; Falcão, A. J.; Farràs Casas, M.; Federici, L.; Fedorets, G.; Fernández-Hernández, J.; Fernique, P.; Fienga, A.; Figueras, F.; Filippi, F.; Findeisen, K.; Fonti, A.; Fouesneau, M.; Fraile, E.; Fraser, M.; Fuchs, J.; Gai, M.; Galleti, S.; Galluccio, L.; Garabato, D.; García-Sedano, F.; Garofalo, A.; Garralda, N.; Gavras, P.; Gerssen, J.; Geyer, R.; Gilmore, G.; Girona, S.; Giuffrida, G.; Gomes, M.; González-Marcos, A.; González-Núñez, J.; González-Vidal, J. J.; Granvik, M.; Guerrier, A.; Guillout, P.; Guiraud, J.; Gúrpide, A.; Gutiérrez-Sánchez, R.; Guy, L. P.; Haigron, R.; Hatzidimitriou, D.; Haywood, M.; Heiter, U.; Helmi, A.; Hobbs, D.; Hofmann, W.; Holl, B.; Holland, G.; Hunt, J. A. S.; Hypki, A.; Icardi, V.; Irwin, M.; Jevardat de Fombelle, G.; Jofré, P.; Jonker, P. G.; Jorissen, A.; Julbe, F.; Karampelas, A.; Kochoska, A.; Kohley, R.; Kolenberg, K.; Kontizas, E.; Koposov, S. E.; Kordopatis, G.; Koubsky, P.; Krone-Martins, A.; Kudryashova, M.; Kull, I.; Bachchan, R. K.; Lacoste-Seris, F.; Lanza, A. F.; Lavigne, J.-B.; Le Poncin-Lafitte, C.; Lebreton, Y.; Lebzelter, T.; Leccia, S.; Leclerc, N.; Lecoeur-Taibi, I.; Lemaitre, V.; Lenhardt, H.; Leroux, F.; Liao, S.; Licata, E.; Lindstrøm, H. E. P.; Lister, T. A.; Livanou, E.; Lobel, A.; Löffler, W.; López, M.; Lorenz, D.; MacDonald, I.; Magalhães Fernandes, T.; Managau, S.; Mann, R. G.; Mantelet, G.; Marchal, O.; Marchant, J. M.; Marconi, M.; Marinoni, S.; Marrese, P. M.; Marschalkó, G.; Marshall, D. J.; Martín-Fleitas, J. M.; Martino, M.; Mary, N.; Matijevič, G.; Mazeh, T.; McMillan, P. J.; Messina, S.; Michalik, D.; Millar, N. R.; Miranda, B. M. H.; Molina, D.; Molinaro, R.; Molinaro, M.; Molnár, L.; Moniez, M.; Montegriffo, P.; Mor, R.; Mora, A.; Morbidelli, R.; Morel, T.; Morgenthaler, S.; Morris, D.; Mulone, A. F.; Muraveva, T.; Musella, I.; Narbonne, J.; Nelemans, G.; Nicastro, L.; Noval, L.; Ordénovic, C.; Ordieres-Meré, J.; Osborne, P.; Pagani, C.; Pagano, I.; Pailler, F.; Palacin, H.; Palaversa, L.; Parsons, P.; Pecoraro, M.; Pedrosa, R.; Pentikäinen, H.; Pichon, B.; Piersimoni, A. M.; Pineau, F.-X.; Plachy, E.; Plum, G.; Poujoulet, E.; Prša, A.; Pulone, L.; Ragaini, S.; Rago, S.; Rambaux, N.; Ramos-Lerate, M.; Ranalli, P.; Rauw, G.; Read, A.; Regibo, S.; Reylé, C.; Ribeiro, R. A.; Rimoldini, L.; Ripepi, V.; Riva, A.; Rixon, G.; Roelens, M.; Romero-Gómez, M.; Rowell, N.; Royer, F.; Ruiz-Dern, L.; Sadowski, G.; Sagristà Sellés, T.; Sahlmann, J.; Salgado, J.; Salguero, E.; Sarasso, M.; Savietto, H.; Schultheis, M.; Sciacca, E.; Segol, M.; Segovia, J. C.; Segransan, D.; Shih, I.-C.; Smareglia, R.; Smart, R. L.; Solano, E.; Solitro, F.; Sordo, R.; Soria Nieto, S.; Souchay, J.; Spagna, A.; Spoto, F.; Stampa, U.; Steele, I. A.; Steidelmüller, H.; Stephenson, C. A.; Stoev, H.; Suess, F. F.; Süveges, M.; Surdej, J.; Szabados, L.; Szegedi-Elek, E.; Tapiador, D.; Taris, F.; Tauran, G.; Taylor, M. B.; Teixeira, R.; Terrett, D.; Tingley, B.; Trager, S. C.; Turon, C.; Ulla, A.; Utrilla, E.; Valentini, G.; van Elteren, A.; Van Hemelryck, E.; van Leeuwen, M.; Varadi, M.; Vecchiato, A.; Veljanoski, J.; Via, T.; Vicente, D.; Vogt, S.; Voss, H.; Votruba, V.; Voutsinas, S.; Walmsley, G.; Weiler, M.; Weingrill, K.; Wevers, T.; Wyrzykowski, Ł.; Yoldas, A.; Žerjal, M.; Zucker, S.; Zurbach, C.; Zwitter, T.; Alecu, A.; Allen, M.; Allende Prieto, C.; Amorim, A.; Anglada-Escudé, G.; Arsenijevic, V.; Azaz, S.; Balm, P.; Beck, M.; Bernstein, H.-H.; Bigot, L.; Bijaoui, A.; Blasco, C.; Bonfigli, M.; Bono, G.; Boudreault, S.; Bressan, A.; Brown, S.; Brunet, P.-M.; Bunclark, P.; Buonanno, R.; Butkevich, A. G.; Carret, C.; Carrion, C.; Chemin, L.; Chéreau, F.; Corcione, L.; Darmigny, E.; de Boer, K. S.; de Teodoro, P.; de Zeeuw, P. T.; Delle Luche, C.; Domingues, C. D.; Dubath, P.; Fodor, F.; Frézouls, B.; Fries, A.; Fustes, D.; Fyfe, D.; Gallardo, E.; Gallegos, J.; Gardiol, D.; Gebran, M.; Gomboc, A.; Gómez, A.; Grux, E.; Gueguen, A.; Heyrovsky, A.; Hoar, J.; Iannicola, G.; Isasi Parache, Y.; Janotto, A.-M.; Joliet, E.; Jonckheere, A.; Keil, R.; Kim, D.-W.; Klagyivik, P.; Klar, J.; Knude, J.; Kochukhov, O.; Kolka, I.; Kos, J.; Kutka, A.; Lainey, V.; LeBouquin, D.; Liu, C.; Loreggia, D.; Makarov, V. V.; Marseille, M. G.; Martayan, C.; Martinez-Rubi, O.; Massart, B.; Meynadier, F.; Mignot, S.; Munari, U.; Nguyen, A.-T.; Nordlander, T.; Ocvirk, P.; O'Flaherty, K. S.; Olias Sanz, A.; Ortiz, P.; Osorio, J.; Oszkiewicz, D.; Ouzounis, A.; Palmer, M.; Park, P.; Pasquato, E.; Peltzer, C.; Peralta, J.; Péturaud, F.; Pieniluoma, T.; Pigozzi, E.; Poels, J.; Prat, G.; Prod'homme, T.; Raison, F.; Rebordao, J. M.; Risquez, D.; Rocca-Volmerange, B.; Rosen, S.; Ruiz-Fuertes, M. I.; Russo, F.; Sembay, S.; Serraller Vizcaino, I.; Short, A.; Siebert, A.; Silva, H.; Sinachopoulos, D.; Slezak, E.; Soffel, M.; Sosnowska, D.; Straižys, V.; ter Linden, M.; Terrell, D.; Theil, S.; Tiede, C.; Troisi, L.; Tsalmantza, P.; Tur, D.; Vaccari, M.; Vachier, F.; Valles, P.; Van Hamme, W.; Veltz, L.; Virtanen, J.; Wallut, J.-M.; Wichmann, R.; Wilkinson, M. I.; Ziaeepour, H.; Zschocke, S.

    2016-11-01

    Context. At about 1000 days after the launch of Gaia we present the first Gaia data release, Gaia DR1, consisting of astrometry and photometry for over 1 billion sources brighter than magnitude 20.7. Aims: A summary of Gaia DR1 is presented along with illustrations of the scientific quality of the data, followed by a discussion of the limitations due to the preliminary nature of this release. Methods: The raw data collected by Gaia during the first 14 months of the mission have been processed by the Gaia Data Processing and Analysis Consortium (DPAC) and turned into an astrometric and photometric catalogue. Results: Gaia DR1 consists of three components: a primary astrometric data set which contains the positions, parallaxes, and mean proper motions for about 2 million of the brightest stars in common with the Hipparcos and Tycho-2 catalogues - a realisation of the Tycho-Gaia Astrometric Solution (TGAS) - and a secondary astrometric data set containing the positions for an additional 1.1 billion sources. The second component is the photometric data set, consisting of mean G-band magnitudes for all sources. The G-band light curves and the characteristics of 3000 Cepheid and RR Lyrae stars, observed at high cadence around the south ecliptic pole, form the third component. For the primary astrometric data set the typical uncertainty is about 0.3 mas for the positions and parallaxes, and about 1 mas yr-1 for the proper motions. A systematic component of 0.3 mas should be added to the parallax uncertainties. For the subset of 94 000 Hipparcos stars in the primary data set, the proper motions are much more precise at about 0.06 mas yr-1. For the secondary astrometric data set, the typical uncertainty of the positions is 10 mas. The median uncertainties on the mean G-band magnitudes range from the mmag level to 0.03 mag over the magnitude range 5 to 20.7. Conclusions: Gaia DR1 is an important milestone ahead of the next Gaia data release, which will feature five

  1. Temporal profiles of synaptic plasticity-related signals in adult mouse hippocampus with methotrexate treatment

    Institute of Scientific and Technical Information of China (English)

    Miyoung Yang; Juhwan Kim; Sung-Ho Kim; Joong-Sun Kim; Taekyun Shin; Changjong Moon

    2012-01-01

    Methotrexate, which is used to treat many malignancies and autoimmune diseases, affects brain functions including hippocampal-dependent memory function. However, the precise mechanisms underlying methotrexate-induced hippocampal dysfunction are poorly understood. To evaluate temporal changes in synaptic plasticity-related signals, the expression and activity of N-methyl-D-aspartic acid receptor 1, calcium/calmodulin-dependent protein kinase II, extracellular signal-regulated kinase 1/2, cAMP responsive element-binding protein, glutamate receptor 1, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor were examined in the hippocampi of adult C57BL/6 mice after methotrexate (40 mg/kg) intraperitoneal injection. Western blot analysis showed biphasic changes in synaptic plasticity-related signals in adult hippocampi following methotrexate treatment. N-methyl-D-aspartic acid receptor 1, cal-cium/calmodulin-dependent protein kinase II, and glutamate receptor 1 were acutely activated dur-ing the early phase (1 day post-injection), while extracellular signal-regulated kinase 1/2 and cAMP responsive element-binding protein activation showed biphasic increases during the early (1 day post-injection) and late phases (7-14 days post-injection). Brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor expression increased significantly during the late phase (7-14 days post-injection). Therefore, methotrexate treatment affects synaptic plasticity-related signals in the adult mouse hippocampus, suggesting that changes in synaptic plasticity-related signals may be associated with neuronal survival and plasticity-related cellular remodeling.

  2. Temporal profiles of synaptic plasticity-related signals in adult mouse hippocampus with methotrexate treatment.

    Science.gov (United States)

    Yang, Miyoung; Kim, Juhwan; Kim, Sung-Ho; Kim, Joong-Sun; Shin, Taekyun; Moon, Changjong

    2012-07-25

    Methotrexate, which is used to treat many malignancies and autoimmune diseases, affects brain functions including hippocampal-dependent memory function. However, the precise mechanisms underlying methotrexate-induced hippocampal dysfunction are poorly understood. To evaluate temporal changes in synaptic plasticity-related signals, the expression and activity of N-methyl-D-aspartic acid receptor 1, calcium/calmodulin-dependent protein kinase II, extracellular signal-regulated kinase 1/2, cAMP responsive element-binding protein, glutamate receptor 1, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor were examined in the hippocampi of adult C57BL/6 mice after methotrexate (40 mg/kg) intraperitoneal injection. Western blot analysis showed biphasic changes in synaptic plasticity-related signals in adult hippocampi following methotrexate treatment. N-methyl-D-aspartic acid receptor 1, calcium/calmodulin-dependent protein kinase II, and glutamate receptor 1 were acutely activated during the early phase (1 day post-injection), while extracellular signal-regulated kinase 1/2 and cAMP responsive element-binding protein activation showed biphasic increases during the early (1 day post-injection) and late phases (7-14 days post-injection). Brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor expression increased significantly during the late phase (7-14 days post-injection). Therefore, methotrexate treatment affects synaptic plasticity-related signals in the adult mouse hippocampus, suggesting that changes in synaptic plasticity-related signals may be associated with neuronal survival and plasticity-related cellular remodeling.

  3. Molecular profiling of signalling proteins for effects induced by the anti-cancer compound GSAO with 400 antibodies

    Directory of Open Access Journals (Sweden)

    Harris Adrian L

    2006-06-01

    Full Text Available Abstract Background GSAO (4-[N-[S-glutathionylacetyl]amino] phenylarsenoxide is a hydrophilic derivative of the protein tyrosine phosphatase inhibitor phenylarsine oxide (PAO. It inhibits angiogenesis and tumour growth in mouse models and may be evaluated in a phase I clinical trial in the near future. Initial experiments have implicated GSAO in perturbing mitochondrial function. Other molecular effects of GSAO in human cells, for example on the phosphorylation of proteins, are still largely unknown. Methods Peripheral white blood cells (PWBC from healthy volunteers were isolated and used to profile effects of GSAO vs. a control compound, GSCA. Changes in site-specific phosphorylations, other protein modifications and expression levels of many signalling proteins were analysed using more than 400 different antibodies in Western blots. Results PWBC were initially cultured in low serum conditions, with the aim to reduce basal protein phosphorylation and to increase detection sensitivity. Under these conditions pleiotropic intracellular signalling protein changes were induced by GSAO. Subsequently, PWBC were cultured in 100% donor serum to reflect more closely in vivo conditions. This eliminated detectable GSAO effects on most, but not all signalling proteins analysed. Activation of the MAP kinase Erk2 was still observed and the paxillin homologue Hic-5 still displayed a major shift in protein mobility upon GSAO-treatment. A GSAO induced change in Hic-5 mobility was also found in endothelial cells, which are thought to be the primary target of GSAO in vivo. Conclusion Serum conditions greatly influence the molecular activity profile of GSAO in vitro. Low serum culture, which is typically used in experiments analysing protein phosphorylation, is not suitable to study GSAO activity in cells. The signalling proteins affected by GSAO under high serum conditions are candidate surrogate markers for GSAO bioactivity in vivo and can be analysed in future

  4. Concept and approach of human signal-molecular-profiling database: a pilot study on depression using Lab-on-chips.

    Science.gov (United States)

    Dong, Tao; Zhao, Xinyan; Yang, Zhaochu

    2013-01-01

    Signal molecular profiling (SMP) in serum can reveal abundant medical information about the human body. The construction of a human signal-molecular-profiling database (HSMPD) will greatly prompt the research of medical science. However, some challenges hinder the construction of HSMPD. A promising strategy is proposed to provide a convenient way for the establishment of HSMPD. Firstly, a low-cost and high-throughput tool for measuring SMP should be developed and standardized. When the SMP-oriented tools were accepted by most hospitals worldwide, SMP information will be decoded by a cloud-based system and stored into the online database naturally. In the pilot study, an ultrasensitive Lab-on-chips (LOC) device was developed as a specific tool for SMP. Clinical serum samples from 10 women within 4 weeks of giving birth, including 2 patients with postpartum depression were studied by the LOC devices, since accumulating evidence has indicated that hormones and cytokines in patients with mood disorders are abnormal. HSMPD may be applied to diagnose depression in the future. Here, five kinds of signal molecules were quantified on the devices, namely, tumor necrosis factor-alpha (TNF-α), thyroid-stimulating hormone (TSH), interleukin (IL)-2, IL-6 and IL-8. The preliminary results showed that the concentrations of IL-2 and IL-8 in the depression group may be higher than those in the control group, whereas the other kinds of signal molecules did not change significantly. Although the correlations are not enough to induct any diagnostic criterion, the SMP-oriented tool was verified. The results also indicated that the strategy to establish HSMPD is conceivable.

  5. Soil profiles' development and differentiation as revealed by their magnetic signal

    Science.gov (United States)

    Jordanova, Neli; Jordanova, Diana

    2017-04-01

    Soil profiles' development is a major theme in soil science research, as far as it gives basic information on soil genesis and classification. The use of soil magnetic properties as indicators for physical and geochemical conditions during pedogenesis received great attention during the last decade mainly in relation to paleoclimate reconstructions. However, tracking the observed general relationships with respect to degree of soil differentiation would lead to capitalization of this knowledge and its further utilization as pedogenic indicator. Here we present an overview of the observed relationships and depth variations of magnetic characteristics along ten soil profiles of Chernozems, Luvisols and Planosols from Bulgaria. Depending on the general soil group considered, different relationships between depth distribution of the relative amount of superparamagnetic (SP), single domain (SD) and larger pseudo single domain (PSD) to multi domain (MD) ferrimagnetic fractions are revealed. The profiles of the soil group with pronounced accumulation of organic matter in the mineral topsoil (Chernozems and Phaeozems) a systematic shift in the relative maxima of SP- and SD- like concentration proxies is observed with the increase of profile differentiation. In contrast, the group of soils with clay-enriched subsoil horizon (e.g. Luvisols) shows different evolution of the depth distribution of the grain-size proxy parameters. The increase of profile's degradation leads to a decrease in the amount of the SP fraction and a split in its maxima into two depth intervals related to the eluvial and illuvial horizons respectively. Along with this tendency, the maximum of the SD fraction moves to progressively deeper levels of the illuvial horizon. The third soil group of the Planosols is characterized by specific re-distribution of the iron oxides, caused by the oscillating oxidation - reduction fluctuations within the profile. The diagnostic eluvial and illuvial soil horizons are

  6. Phosphoproteomic profiling of in vivo signaling in liver by the mammalian target of rapamycin complex 1 (mTORC1.

    Directory of Open Access Journals (Sweden)

    Gokhan Demirkan

    Full Text Available Our understanding of signal transduction networks in the physiological context of an organism remains limited, partly due to the technical challenge of identifying serine/threonine phosphorylated peptides from complex tissue samples. In the present study, we focused on signaling through the mammalian target of rapamycin (mTOR complex 1 (mTORC1, which is at the center of a nutrient- and growth factor-responsive cell signaling network. Though studied extensively, the mechanisms involved in many mTORC1 biological functions remain poorly understood.We developed a phosphoproteomic strategy to purify, enrich and identify phosphopeptides from rat liver homogenates. Using the anticancer drug rapamycin, the only known target of which is mTORC1, we characterized signaling in liver from rats in which the complex was maximally activated by refeeding following 48 hr of starvation. Using protein and peptide fractionation methods, TiO(2 affinity purification of phosphopeptides and mass spectrometry, we reproducibly identified and quantified over four thousand phosphopeptides. Along with 5 known rapamycin-sensitive phosphorylation events, we identified 62 new rapamycin-responsive candidate phosphorylation sites. Among these were PRAS40, gephyrin, and AMP kinase 2. We observed similar proportions of increased and reduced phosphorylation in response to rapamycin. Gene ontology analysis revealed over-representation of mTOR pathway components among rapamycin-sensitive phosphopeptide candidates.In addition to identifying potential new mTORC1-mediated phosphorylation events, and providing information relevant to the biology of this signaling network, our experimental and analytical approaches indicate the feasibility of large-scale phosphoproteomic profiling of tissue samples to study physiological signaling events in vivo.

  7. An Advance Open Architecture Astrometric Alignment Sensor for Distributed & Non-Distributed GN&C Systems Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This IRAD will advance the technology to provide a stellar sensor that may be used for astrometric alignments necessary for Formation Flying/Relative Navigation...

  8. Global phosphoproteomic profiling reveals perturbed signaling in a mouse model of dilated cardiomyopathy

    Science.gov (United States)

    Kuzmanov, Uros; Guo, Hongbo; Buchsbaum, Diana; Cosme, Jake; Abbasi, Cynthia; Isserlin, Ruth; Sharma, Parveen; Gramolini, Anthony O.; Emili, Andrew

    2016-01-01

    Phospholamban (PLN) plays a central role in Ca2+ homeostasis in cardiac myocytes through regulation of the sarco(endo)plasmic reticulum Ca2+-ATPase 2A (SERCA2A) Ca2+ pump. An inherited mutation converting arginine residue 9 in PLN to cysteine (R9C) results in dilated cardiomyopathy (DCM) in humans and transgenic mice, but the downstream signaling defects leading to decompensation and heart failure are poorly understood. Here we used precision mass spectrometry to study the global phosphorylation dynamics of 1,887 cardiac phosphoproteins in early affected heart tissue in a transgenic R9C mouse model of DCM compared with wild-type littermates. Dysregulated phosphorylation sites were quantified after affinity capture and identification of 3,908 phosphopeptides from fractionated whole-heart homogenates. Global statistical enrichment analysis of the differential phosphoprotein patterns revealed selective perturbation of signaling pathways regulating cardiovascular activity in early stages of DCM. Strikingly, dysregulated signaling through the Notch-1 receptor, recently linked to cardiomyogenesis and embryonic cardiac stem cell development and differentiation but never directly implicated in DCM before, was a prominently perturbed pathway. We verified alterations in Notch-1 downstream components in early symptomatic R9C transgenic mouse cardiomyocytes compared with wild type by immunoblot analysis and confocal immunofluorescence microscopy. These data reveal unexpected connections between stress-regulated cell signaling networks, specific protein kinases, and downstream effectors essential for proper cardiac function. PMID:27742792

  9. Analysis of Human TAAR8 and Murine Taar8b Mediated Signaling Pathways and Expression Profile

    Directory of Open Access Journals (Sweden)

    Jessica Mühlhaus

    2014-11-01

    Full Text Available The thyroid hormone derivative 3-iodothyronamine (3-T1AM exerts metabolic effects in vivo that contradict known effects of thyroid hormones. 3-T1AM acts as a trace amine-associated receptor 1 (TAAR1 agonist and activates Gs signaling in vitro. Interestingly, 3-T1AM-meditated in vivo effects persist in Taar1 knockout-mice indicating that further targets of 3-T1AM might exist. Here, we investigated another member of the TAAR family, the only scarcely studied mouse and human trace-amine-associated receptor 8 (Taar8b, TAAR8. By RT-qPCR and locked-nucleic-acid (LNA in situ hybridization, Taar8b expression in different mouse tissues was analyzed. Functionally, we characterized TAAR8 and Taar8b with regard to cell surface expression and signaling via different G-protein-mediated pathways. Cell surface expression was verified by ELISA, and cAMP accumulation was quantified by AlphaScreen for detection of Gs and/or Gi/o signaling. Activation of G-proteins Gq/11 and G12/13 was analyzed by reporter gene assays. Expression analyses revealed at most marginal Taar8b expression and no gender differences for almost all analyzed tissues. In heart, LNA-in situ hybridization demonstrated the absence of Taar8b expression. We could not identify 3-T1AM as a ligand for TAAR8 and Taar8b, but both receptors were characterized by a basal Gi/o signaling activity, a so far unknown signaling pathway for TAARs.

  10. Astrometric positions for 18 irregular satellites of giant planets from 23 years of observations

    CERN Document Server

    Gomes-Júnior, A R; Vieira-Martins, R; Arlot, J -E; Camargo, J I B; Braga-Ribas, F; Neto, D N da Silva; Andrei, A H; Dias-Oliveira, A; Morgado, B E; Benedetti-Rossi, G; Duchemin, Y; Desmars, J; Lainey, V; Thuillot, W

    2015-01-01

    The irregular satellites of the giant planets are believed to have been captured during the evolution of the solar system. Knowing their physical parameters, such as size, density, and albedo is important for constraining where they came from and how they were captured. The best way to obtain these parameters are observations in situ by spacecrafts or from stellar occultations by the objects. Both techniques demand that the orbits are well known. We aimed to obtain good astrometric positions of irregular satellites to improve their orbits and ephemeris. We identified and reduced observations of several irregular satellites from three databases containing more than 8000 images obtained between 1992 and 2014 at three sites (Observat\\'orio do Pico dos Dias, Observatoire de Haute-Provence, and European Southern Observatory - La Silla). We used the software PRAIA (Platform for Reduction of Astronomical Images Automatically) to make the astrometric reduction of the CCD frames. The UCAC4 catalog represented the Inte...

  11. Astrometric radial velocities. I. Non-spectroscopic methods for measuring stellar radial velocity

    Science.gov (United States)

    Dravins, Dainis; Lindegren, Lennart; Madsen, Søren

    1999-08-01

    High-accuracy astrometry permits the determination of not only stellar tangential motion, but also the component along the line-of-sight. Such non-spectroscopic (i.e. astrometric) radial velocities are independent of stellar atmospheric dynamics, spectral complexity and variability, as well as of gravitational redshift. Three methods are analysed: (1) changing annual parallax, (2) changing proper motion and (3) changing angular extent of a moving group of stars. All three have significant potential in planned astrometric projects. Current accuracies are still inadequate for the first method, while the second is marginally feasible and is here applied to 16 stars. The third method reaches high accuracy (accuracy limit is set by uncertainties in the cluster expansion rate. Based (in part) on observations by the ESA Hipparcos satellite

  12. Astrometric radial velocities; 1, Non-spectroscopic methods for measuring stellar radial velocity

    CERN Document Server

    Dravins, D; Madsen, S; Dravins, Dainis; Lindegren, Lennart; Madsen, Soren

    1999-01-01

    High-accuracy astrometry permits the determination of not only stellar tangential motion, but also the component along the line-of-sight. Such non-spectroscopic (i.e. astrometric) radial velocities are independent of stellar atmospheric dynamics, spectral complexity and variability, as well as of gravitational redshift. Three methods are analysed: (1) changing annual parallax, (2) changing proper motion and (3) changing angular extent of a moving group of stars. All three have significant potential in planned astrometric projects. Current accuracies are still inadequate for the first method, while the second is marginally feasible and is here applied to 16 stars. The third method reaches high accuracy (<1 km/s) already with present data, although for some clusters an accuracy limit is set by uncertainties in the cluster expansion rate.

  13. A Global Astrometric Solution for Pan-STARRS Referenced to ICRF2

    Science.gov (United States)

    Berghea, C. T.; Makarov, V. V.; Frouard, J.; Hennessy, G. S.; Dorland, B. N.; Veillette, D. R.; Dudik, R. P.; Magnier, E. A.; Burgett, W. S.; Chambers, K. C.; Denneau, L.; Flewelling, H.; Kaiser, N.; Tonry, J. L.; Wainscoat, R. J.; Sesar, B.

    2016-09-01

    We describe the development and application of a Global Astrometric Solution (GAS) to the problem of Pan-STARRS1 (PS1) astrometry. Current PS1 astrometry is based on differential astrometric measurements using 2MASS reference stars, and thus PS1 astrometry inherits the errors of the 2MASS catalog. The GAS, based on a single, least-squares adjustment to approximately 750 k “grid stars” using over 3000 extragalactic objects as reference objects, avoids this catalog-to-catalog propagation of errors to a great extent. The GAS uses a relatively small number of quasi-stellar objects (QSOs, or distant active galactic nuclei) with very accurate (expected based on simulations (˜10 mas). We provide a likely explanation for the reason the small-scale residual errors are not corrected in our solution as would be expected.

  14. Improving distances to nearby bright stars: Combining astrometric data from Hipparcos, Nano-JASMINE and Gaia

    CERN Document Server

    Michalik, Daniel; Hobbs, David; Lammers, Uwe; Yamada, Yoshiyuki

    2014-01-01

    Starting in 2013, Gaia will deliver highly accurate astrometric data, which eventually will supersede most other stellar catalogues in accuracy and completeness. It is, however, lim- ited to observations from magnitude 6 to 20 and will therefore not include the brightest stars. Nano-JASMINE, an ultrasmall Japanese astrometry satellite, will observe these bright stars, but with much lower accuracy. Hence, the Hipparcos catalogue from 1997 will likely remain the main source of accurate distances to bright nearby stars. We are investigating how this might be improved by optimally combining data from all three missions in a joint astrometric solu- tion. This would take advantage of the unique features of each mission: the historic bright-star measurements of Hipparcos, the updated bright-star observations of Nano-JASMINE, and the very accurate reference frame of Gaia. The long temporal baseline between the missions pro- vides additional benefits for the determination of proper motions and binary detection, which ...

  15. Transcriptional Profiling of the Oral Pathogen Streptococcus mutans in Response to Competence Signaling Peptide XIP

    Science.gov (United States)

    Wenderska, Iwona B.; Latos, Andrew; Pruitt, Benjamin; Palmer, Sara; Spatafora, Grace

    2017-01-01

    ABSTRACT In the cariogenic Streptococcus mutans, competence development is regulated by the ComRS signaling system comprised of the ComR regulator and the ComS prepeptide to the competence signaling peptide XIP (ComX-inducing peptide). Aside from competence development, XIP signaling has been demonstrated to regulate cell lysis, and recently, the expression of bacteriocins, small antimicrobial peptides used by bacteria to inhibit closely related species. Our study further explores the effect of XIP signaling on the S. mutans transcriptome. RNA sequencing revealed that XIP induction resulted in a global change in gene expression that was consistent with a stress response. An increase in several membrane-bound regulators, including HdrRM and BrsRM, involved in bacteriocin production, and the VicRKX system, involved in acid tolerance and biofilm formation, was observed. Furthermore, global changes in gene expression corresponded to changes observed during the stringent response to amino acid starvation. Effects were also observed on genes involved in sugar transport and carbon catabolite repression and included the levQRST and levDEFG operons. Finally, our work identified a novel heat shock-responsive intergenic region, encoding a small RNA, with a potential role in competence shutoff. IMPORTANCE Genetic competence provides bacteria with an opportunity to increase genetic diversity or acquire novel traits conferring a survival advantage. In the cariogenic pathogen Streptococcus mutans, DNA transformation is regulated by the competence stimulating peptide XIP (ComX-inducing peptide). The present study utilizes high-throughput RNA sequencing (RNAseq) to provide a greater understanding of how global gene expression patterns change in response to XIP. Overall, our work demonstrates that in S. mutans, XIP signaling induces a response that resembles the stringent response to amino acid starvation. We further identify a novel heat shock-responsive intergenic region with a

  16. Astrometric radial velocities. I. Non-spectroscopic methods for measuring stellar radial velocity

    OpenAIRE

    1999-01-01

    High-accuracy astrometry permits the determination of not only stellar tangential motion, but also the component along the line-of-sight. Such non-spectroscopic (i.e. astrometric) radial velocities are independent of stellar atmospheric dynamics, spectral complexity and variability, as well as of gravitational redshift. Three methods are analysed: (1) changing annual parallax, (2) changing proper motion and (3) changing angular extent of a moving group of stars. All three have significant pot...

  17. Social environment has a primary influence on the microbial and odor profiles of a chemically signaling songbird

    Directory of Open Access Journals (Sweden)

    Danielle June Whittaker

    2016-08-01

    Full Text Available Chemical signaling is an underappreciated means of communication among birds, as may be the potential contributions of symbiotic microbes to animal chemical communication in general. The dark-eyed junco (Junco hyemalis produces and detects volatile compounds that may be important in reproductive behavior. These compounds are found in preen oil secreted by the uropygial gland, and this gland supports diverse bacterial communities including genera known to produce some of these volatile compounds. We investigated the relative contributions of shared environments and genetic relatedness in shaping juncos’ symbiotic bacterial communities, and investigated whether these bacterial communities underlie juncos’ chemical signaling behavior. We sampled parents and nestlings at 9 junco nests during one breeding season at Mountain Lake Biological Station in Virginia, USA. From each individual, we collected swabs of the uropygial gland and the cloaca, preen oil, and a small blood sample for paternity testing. We characterized junco bacterial communities through 16S rRNA gene surveys and preen oil volatile compounds via gas chromatography-mass spectrometry. Nest membership and age class had the strongest influence on the structure of bacterial and volatile profiles. We compared father-offspring similarity based on paternity, and nestling similarity in nests containing full siblings and half siblings, and found that relatedness did not noticeably affect bacterial or volatile profiles. While we cannot rule out an influence of genetic relatedness on these profiles, it is clear that shared environments are more influential in shaping bacterial and volatile profiles among juncos.We did not find significant covariation between individual bacterial and volatile profiles. Possible explanations for this result include: 1 bacteria do not underlie volatile production; 2 ample redundancy in volatile production among bacterial types obscures covariation; or 3 the

  18. Global microRNA profiles and signaling pathways in the development of cardiac hypertrophy

    Energy Technology Data Exchange (ETDEWEB)

    Feng, H.J.; Ouyang, W.; Liu, J.H.; Sun, Y.G.; Hu, R.; Huang, L.H.; Xian, J.L. [Southern Medical University, Department of Nuclear Medicine, Zhujiang Hospital, Guangzhou, China, Department of Nuclear Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou (China); Jing, C.F.; Zhou, M.J. [Sun Yat-Sen University, South China Sea Marine Biotechnology, National Engineering Research Center, Guangzhou, China, National Engineering Research Center, South China Sea Marine Biotechnology, Sun Yat-Sen University, Guangzhou (China)

    2014-04-11

    Hypertrophy is a major predictor of progressive heart disease and has an adverse prognosis. MicroRNAs (miRNAs) that accumulate during the course of cardiac hypertrophy may participate in the process. However, the nature of any interaction between a hypertrophy-specific signaling pathway and aberrant expression of miRNAs remains unclear. In this study, Spague Dawley male rats were treated with transverse aortic constriction (TAC) surgery to mimic pathological hypertrophy. Hearts were isolated from TAC and sham operated rats (n=5 for each group at 5, 10, 15, and 20 days after surgery) for miRNA microarray assay. The miRNAs dysexpressed during hypertrophy were further analyzed using a combination of bioinformatics algorithms in order to predict possible targets. Increased expression of the target genes identified in diverse signaling pathways was also analyzed. Two sets of miRNAs were identified, showing different expression patterns during hypertrophy. Bioinformatics analysis suggested the miRNAs may regulate multiple hypertrophy-specific signaling pathways by targeting the member genes and the interaction of miRNA and mRNA might form a network that leads to cardiac hypertrophy. In addition, the multifold changes in several miRNAs suggested that upregulation of rno-miR-331*, rno-miR-3596b, rno-miR-3557-5p and downregulation of rno-miR-10a, miR-221, miR-190, miR-451 could be seen as biomarkers of prognosis in clinical therapy of heart failure. This study described, for the first time, a potential mechanism of cardiac hypertrophy involving multiple signaling pathways that control up- and downregulation of miRNAs. It represents a first step in the systematic discovery of miRNA function in cardiovascular hypertrophy.

  19. Multi-OMIC profiling of survival and metabolic signaling networks in cells subjected to photodynamic therapy.

    Science.gov (United States)

    Weijer, Ruud; Clavier, Séverine; Zaal, Esther A; Pijls, Maud M E; van Kooten, Robert T; Vermaas, Klaas; Leen, René; Jongejan, Aldo; Moerland, Perry D; van Kampen, Antoine H C; van Kuilenburg, André B P; Berkers, Celia R; Lemeer, Simone; Heger, Michal

    2017-03-01

    Photodynamic therapy (PDT) is an established palliative treatment for perihilar cholangiocarcinoma that is clinically promising. However, tumors tend to regrow after PDT, which may result from the PDT-induced activation of survival pathways in sublethally afflicted tumor cells. In this study, tumor-comprising cells (i.e., vascular endothelial cells, macrophages, perihilar cholangiocarcinoma cells, and EGFR-overexpressing epidermoid cancer cells) were treated with the photosensitizer zinc phthalocyanine that was encapsulated in cationic liposomes (ZPCLs). The post-PDT survival pathways and metabolism were studied following sublethal (LC50) and supralethal (LC90) PDT. Sublethal PDT induced survival signaling in perihilar cholangiocarcinoma (SK-ChA-1) cells via mainly HIF-1-, NF-кB-, AP-1-, and heat shock factor (HSF)-mediated pathways. In contrast, supralethal PDT damage was associated with a dampened survival response. PDT-subjected SK-ChA-1 cells downregulated proteins associated with EGFR signaling, particularly at LC90. PDT also affected various components of glycolysis and the tricarboxylic acid cycle as well as metabolites involved in redox signaling. In conclusion, sublethal PDT activates multiple pathways in tumor-associated cell types that transcriptionally regulate cell survival, proliferation, energy metabolism, detoxification, inflammation/angiogenesis, and metastasis. Accordingly, tumor cells sublethally afflicted by PDT are a major therapeutic culprit. Our multi-omic analysis further unveiled multiple druggable targets for pharmacological co-intervention.

  20. Astrometric performance of the Gemini multi-conjugate adaptive optics system in crowded fields

    CERN Document Server

    Neichel, Benoit; Rigaut, Francois; Ammons, S Mark; Carrasco, Eleazar R; Lassalle, Emmanuel

    2014-01-01

    The Gemini Multi-conjugate adaptive optics System (GeMS) is a facility instrument for the Gemini-South telescope. It delivers uniform, near-diffraction-limited image quality at near-infrared wavelengths over a 2 arcminute field of view. Together with the Gemini South Adaptive Optics Imager (GSAOI), a near-infrared wide field camera, GeMS/GSAOI's combination of high spatial resolution and a large field of view will make it a premier facility for precision astrometry. Potential astrometric science cases cover a broad range of topics including exo-planets, star formation, stellar evolution, star clusters, nearby galaxies, black holes and neutron stars, and the Galactic center. In this paper, we assess the astrometric performance and limitations of GeMS/GSAOI. In particular, we analyze deep, mono-epoch images, multi-epoch data and distortion calibration. We find that for single-epoch, un-dithered data, an astrometric error below 0.2 mas can be achieved for exposure times exceeding one minute, provided enough star...

  1. Tycho-Gaia Astrometric Solution parallaxes and proper motions for 5 Galactic globular clusters

    CERN Document Server

    Watkins, Laura L

    2016-01-01

    We perform a systematic search for Galactic globular cluster (GC) stars in the Tycho-Gaia Astrometric Solution (TGAS) catalogue that formed part of Gaia Data Release 1 (DR1), and identify 5 members of NGC104 (47 Tucanae), 1 member of NGC5272 (M3), 5 members of NGC6121 (M4), 7 members of NGC6397, and 2 members of NGC6656 (M22). By taking a weighted average of the member stars, fully accounting for the correlations between parameter estimates, we estimate the parallax (and, hence, distance) and proper motion (PM) of the GCs. This provides a homogeneous PM study of multiple GCs based on an astrometric catalog with small and well-controlled systematic errors, and yields random PM errors that are similar to existing measurements. Detailed comparison to the available Hubble Space Telescope (HST) measurements generally shows excellent agreement, validating the astrometric quality of both TGAS and HST. By contrast, comparison to ground-based measurements shows that some of those must have systematic errors exceeding ...

  2. Using HST to Detect Isolated Black Holes and Neutron Stars through Astrometric Microlensing

    Science.gov (United States)

    Sahu, Kailash C.; Albrow, M.; Anderson, J.; Bond, H. E.; Bond, I.; Brown, T. M.; Casertano, S.; Dominik, M.; Ferguson, H. C.; Fryer, C.; Livio, M.; Mao, S.; Perrott, Y.; Udalski, A.; Yock, P.

    2012-05-01

    To date, Black Hole (BH) and Neutron Star (NS) masses have been directly measured only in binaries; no isolated stellar-mass BH has been detected unambiguously within our Galaxy. We have underway a large, 3-year HST program (192 orbits) designed to detect microlensing events caused by non-luminous isolated BHs and NSs in the direction of the Galactic bulge. Our program consists of monitoring of 12 fields in the Sagittarius window of the Galactic bulge, containing a total of 1.5 million stars down to V=28. Our observations have a typical cadence of one observation every two weeks, and are primarily targeted towards detecting microlensing events caused by non-luminous isolated BHs and NSs in the Galactic disk and bulge. The unique capability of HST imaging for microlensing observations is the addition of high-precision astrometry, allowing detection of the astrometric shift of the source during the event. Combined with the lens parallax, which can be determined from the light curve as measured by HST (and supplemented by GEMINI) observations, the astrometric shift provides a direct measurement of the lens mass. Our program is optimized to detect long-duration events, which are more likely to be caused by massive lenses. We expect to detect a few dozen long-duration microlensing events, of which 45% will show astrometric deflections, leading to direct determinations of the lens masses.

  3. Gaia Data Release 1. Summary of the astrometric, photometric, and survey properties

    CERN Document Server

    Brown, A G A; Prusti, T; de Bruijne, J; Mignard, F; Drimmel, R; co-authors, 585

    2016-01-01

    At about 1000 days after the launch of Gaia we present the first Gaia data release, Gaia DR1, consisting of astrometry and photometry for over 1 billion sources brighter than magnitude 20.7. We summarize Gaia DR1 and provide illustrations of the scientific quality of the data, followed by a discussion of the limitations due to the preliminary nature of this release. Gaia DR1 consists of: a primary astrometric data set which contains the positions, parallaxes, and mean proper motions for about 2 million of the brightest stars in common with the Hipparcos and Tycho-2 catalogues and a secondary astrometric data set containing the positions for an additional 1.1 billion sources. The second component is the photometric data set,consisting of mean G-band magnitudes for all sources. The G-band light curves and the characteristics of ~3000 Cepheid and RR Lyrae stars, observed at high cadence around the south ecliptic pole, form the third component. For the primary astrometric data set the typical uncertainty is about...

  4. Relativistic formulation of coordinate light time, Doppler and astrometric observables up to the second post-Minkowskian order

    CERN Document Server

    Hees, A; Poncin-Lafitte, C Le

    2014-01-01

    Given the extreme accuracy of modern space science, a precise relativistic modeling of observations is required. In particular, it is important to describe properly light propagation through the Solar System. For two decades, several modeling efforts based on the solution of the null geodesic equations have been proposed but they are mainly valid only for the first order Post-Newtonian approximation. However, with the increasing precision of ongoing space missions as Gaia, GAME, BepiColombo, JUNO or JUICE, we know that some corrections up to the second order have to be taken into account for future experiments. We present a procedure to compute the relativistic coordinate time delay, Doppler and astrometric observables avoiding the integration of the null geodesic equation. This is possible using the Time Transfer Function formalism, a powerful tool providing key quantities such as the time of flight of a light signal between two point-events and the tangent vector to its null-geodesic. Indeed we show how to ...

  5. Using regression models to enhance signals in a dispersive radiative field: Reducing stray light corruption in the limb profiles of the Ozone Mapper Profiler Suite (OMPS)

    Science.gov (United States)

    Bergman, J. W.; Flynn, L. E.; Hornstein, J. S.; Lumpe, J. D.

    2006-12-01

    As the technology of remote sensing using radiometric observations advances and we are confronted with weaker and weaker signals, stray light corruption will become an increasingly important challenge. Stray light is particularly important for instruments that sample a large dynamic range such as the OMPS limb profiler. In that case, even a very small fraction of photons straying from the high-intensity region of spectral/viewing angle space can dominate measurements in the low intensity region. For the OMPS limb profiler, stray light represents a small linear perturbation to the overall observed energy, even though it dominates observations for some wavelengths and viewing angles. By exploiting those characteristics, we have found that both iterative techniques based on a Taylor expansion of the inverse stray light operator and linear regression models can effectively reduce stray light corruption from the OMPS measurements provided there is sufficient sampling of the measured radiant energy. Regression models are extremely efficient in operational application because their cost is incurred during offline training. However, they can perform no better than the data used to train them and only work well for systems whose dynamical operators are largely linear. Regression models can be problematic in an operational application if the instrument undergoes changes (e.g., pixel failure in the detector); with the instrument in orbit, retraining can be difficult. Thus, it is important to both characterize stray light with instrument tests before it is launched and to find stray light removal techniques that are flexible and can be altered to accommodate instrument changes. We examine three variations of an ozone retrieval algorithm that utilizes regression models to characterize photon dispersion and other instrument effects. These three methods are compared in terms of their sensitivity to model error, their sensitivity to errors in the assumed background atmospheric

  6. Quantitative impedimetric NPY-receptor activation monitoring and signal pathway profiling in living cells.

    Science.gov (United States)

    te Kamp, Verena; Lindner, Ricco; Jahnke, Heinz-Georg; Krinke, Dana; Kostelnik, Katja B; Beck-Sickinger, Annette G; Robitzki, Andrea A

    2015-05-15

    Label-free and non-invasive monitoring of receptor activation and identification of the involved signal pathways in living cells is an ongoing analytic challenge and a great opportunity for biosensoric systems. In this context, we developed an impedance spectroscopy-based system for the activation monitoring of NPY-receptors in living cells. Using an optimized interdigital electrode array for sensitive detection of cellular alterations, we were able for the first time to quantitatively detect the NPY-receptor activation directly without a secondary or enhancer reaction like cAMP-stimulation by forskolin. More strikingly, we could show that the impedimetric based NPY-receptor activation monitoring is not restricted to the Y1-receptor but also possible for the Y2- and Y5-receptor. Furthermore, we could monitor the NPY-receptor activation in different cell lines that natively express NPY-receptors and proof the specificity of the observed impedimetric effect by agonist/antagonist studies in recombinant NPY-receptor expressing cell lines. To clarify the nature of the observed impedimetric effect we performed an equivalent circuit analysis as well as analyzed the role of cell morphology and receptor internalization. Finally, an antagonist based extensive molecular signal pathway analysis revealed small alterations of the actin cytoskeleton as well as the inhibition of at least L-type calcium channels as major reasons for the observed NPY-induced impedance increase. Taken together, our novel impedance spectroscopy based NPY-receptor activation monitoring system offers the opportunity to identify signal pathways as well as for novel versatile agonist/antagonist screening systems for identification of novel therapeutics in the field of obesity and cancer.

  7. Determination of global plasmaspheric electron density profile by tomographic approach using omega signals and ray tracing

    Science.gov (United States)

    Kimura, I.; Kasahara, Y.; Oya, H.

    2001-07-01

    It has been necessary requirements to determine the global electron density distribution in the plasmasphere with time resolutions, of less than a day. We have provided solutions to this requirement using the wave normal directions, delay time of Omega signals and the in situ electron density observed on-board the Japanese satellite Akebono (Sawada et al., Journal of Geophysical Research 98(11) (1993) 267, Kimura et al., Advance Space Research 15(2) (1995) 103, Advance Space Research 18(6) (1996) 279, Journal of Atmospheric and Solar-Terrestrial Physics 59 (1997) 1569). The present paper is intended to review our earlier studies.

  8. Gene expression profiling identifies activated growth factor signaling in poor prognosis (Luminal-B estrogen receptor positive breast cancer

    Directory of Open Access Journals (Sweden)

    Conus Nelly M

    2009-06-01

    Full Text Available Abstract Background Within estrogen receptor-positive breast cancer (ER+ BC, the expression levels of proliferation-related genes can define two clinically distinct molecular subtypes. When treated with adjuvant tamoxifen, those ER+ BCs that are lowly proliferative have a good prognosis (luminal-A subtype, however the clinical outcome of those that are highly proliferative is poor (luminal-B subtype. Methods To investigate the biological basis for these observations, gene set enrichment analysis (GSEA was performed using microarray data from 246 ER+ BC samples from women treated with adjuvant tamoxifen monotherapy. To create an in vitro model of growth factor (GF signaling activation, MCF-7 cells were treated with heregulin (HRG, an HER3 ligand. Results We found that a gene set linked to GF signaling was significantly enriched in the luminal-B tumors, despite only 10% of samples over-expressing HER2 by immunohistochemistry. To determine the biological significance of this observation, MCF-7 cells were treated with HRG. These cells displayed phosphorylation of HER2/3 and downstream ERK and S6. Treatment with HRG overcame tamoxifen-induced cell cycle arrest with higher S-phase fraction and increased anchorage independent colony formation. Gene expression profiles of MCF-7 cells treated with HRG confirmed enrichment of the GF signaling gene set and a similar proliferative signature observed in human ER+ BCs resistant to tamoxifen. Conclusion These data demonstrate that activation of GF signaling pathways, independent of HER2 over-expression, could be contributing to the poor prognosis of the luminal-B ER+ BC subtype.

  9. Combination of geo- pedo- and technogenic magnetic and geochemical signals in soil profiles - Diversification and its interpretation: A new approach.

    Science.gov (United States)

    Szuszkiewicz, Marcin; Łukasik, Adam; Magiera, Tadeusz; Mendakiewicz, Maria

    2016-07-01

    Magnetic and geochemical parameters of soils are determined with respect to geology, pedogenesis and anthropopression. Depending on local conditions these factors affect magnetic and geochemical signals simultaneously or in various configurations. We examined four type of soils (Entic Podzol, Eutric Cambisol, Humic Cambisol and Dystric Cambisol) developed on various bedrock (the Tumlin Sandstone, basaltoid, amphibolite and serpentinite, respectively). Our primary aim was to characterize the origin and diversification of the magnetic and geochemical signal in soils in order to distinguish the most reliable methods for correct interpretation of measured parameters. Presented data include selected parameters, both magnetic (mass magnetic susceptibility - χ, frequency-dependent magnetic susceptibility - χfd and thermomagnetic susceptibility measurement - TSM), and geochemical (selected heavy metal contents: Co, Cr, Cu, Fe, Mn, Ni, Pb, Zn). Additionally, the enrichment factor (EF) and index of geoaccumulation (Igeo) were calculated. Our results suggest the following: (1) the χ/Fe ratio may be a reliable indicator for determining changes of magnetic signal origin in soil profiles; (2) magnetic and geochemical signals are simultaneously higher (the increment of χ and lead and zinc was noted) in topsoil horizons because of the deposition of technogenic magnetic particles (TMPs); (3) EF and Igeo evaluated for lead and zinc unambiguously showed anthropogenic influence in terms of increasing heavy metal contents in topsoil regardless of bedrock or soil type; (4) magnetic susceptibility measurements supported by TSM curves for soil samples of different genetic horizons are a helpful tool for interpreting the origin and nature of the mineral phases responsible for the changes of magnetic susceptibility values.

  10. Profiling the metabolic signals involved in chemical communication between microbes using imaging mass spectrometry.

    Science.gov (United States)

    Stasulli, Nikolas M; Shank, Elizabeth A

    2016-09-02

    The ability of microbes to secrete bioactive chemical signals into their environment has been known for over a century. However, it is only in the last decade that imaging mass spectrometry has provided us with the ability to directly visualize the spatial distributions of these microbial metabolites. This technology involves collecting mass spectra from multiple discrete locations across a biological sample, yielding chemical 'maps' that simultaneously reveal the distributions of hundreds of metabolites in two dimensions. Advances in microbial imaging mass spectrometry summarized here have included the identification of novel strain- or coculture-specific compounds, the visualization of biotransformation events (where one metabolite is converted into another by a neighboring microbe), and the implementation of a method to reconstruct the 3D subsurface distributions of metabolites, among others. Here we review the recent literature and discuss how imaging mass spectrometry has spurred novel insights regarding the chemical consequences of microbial interactions.

  11. Functional characterization and developmental expression profiling of gibberellin signalling components in Vitis vinifera.

    Science.gov (United States)

    Acheampong, Atiako Kwame; Hu, Jianhong; Rotman, Ariel; Zheng, Chuanlin; Halaly, Tamar; Takebayashi, Yumiko; Jikumaru, Yusuke; Kamiya, Yuji; Lichter, Amnon; Sun, Tai-Ping; Or, Etti

    2015-03-01

    Gibberellins (GAs) regulate numerous developmental processes in grapevine (Vitis vinifera) such as rachis elongation, fruit set, and fruitlet abscission. The ability of GA to promote berry enlargement has led to its indispensable use in the sternospermocarpic ('seedless') table grape industry worldwide. However, apart from VvGAI1 (VvDELLA1), which regulates internode elongation and fruitfulness, but not berry size of seeded cultivars, little was known about GA signalling in grapevine. We have identified and characterized two additional DELLAs (VvDELLA2 and VvDELLA3), two GA receptors (VvGID1a and VvGID1b), and two GA-specific F-box proteins (VvSLY1a and VvSLY1b), in cv. Thompson seedless. With the exception of VvDELLA3-VvGID1b, all VvDELLAs interacted with the VvGID1s in a GA-dependent manner in yeast two-hybrid assays. Additionally, expression of these grape genes in corresponding Arabidopsis mutants confirmed their functions in planta. Spatiotemporal analysis of VvDELLAs showed that both VvDELLA1 and VvDELLA2 are abundant in most tissues, except in developing fruit where VvDELLA2 is uniquely expressed at high levels, suggesting a key role in fruit development. Our results further suggest that differential organ responses to exogenous GA depend on the levels of VvDELLA proteins and endogenous bioactive GAs. Understanding this interaction will allow better manipulation of GA signalling in grapevine. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  12. Intracellular signalling and plasma hormone profiles associated with the expression of unconditioned and conditioned fear and anxiety in female rats.

    Science.gov (United States)

    Simone, Jonathan J; McCormick, Cheryl M

    2017-02-01

    There is considerable overlap in the neural regions and intracellular signalling pathways implicated in anxiety and fear, although less is known in females. Here, we investigated whether unconditioned and conditioned fear are associated with distinct patterns of expression of extracellular signal-regulated kinase-1 and -2 (ERK1/2), protein kinase B (Akt), and calcineurin (CaN) (proteins that are key regulators of the expression of and/or memory processes of fear and anxiety) in the dorsal and ventral hippocampus, medial prefrontal cortex, and amygdala (important regions in neural fear circuitry) of adult female rats, and used a multivariate approach to find patterns of signalling that might discriminate between the different states of fear. To isolate fear to the conditioned cue from generalized fear to the test context, rats were conditioned to an auditory tone (i.e. tone paired with footshock) and twenty-four hours later exposed to a novel context in the presence or absence of the conditioned cue. A third group that was exposed to the conditioning context without undergoing fear conditioning was included to control for unconditioned responses to the testing procedures, which are anxiogenic. A discriminate function analysis and MANOVA determined that hippocampal signalling best discriminated the three groups from each other. The addition of values for plasma concentrations of corticosterone and progesterone (as indices of activation of the hypothalamic-pituitary-adrenal stress axis) to statistical analyses increased the separation of the three groups. There was high degree of association among the three signalling molecules in the four brain regions within each group. There was an absence of the associations between the medial prefrontal cortex and the amygdala in the cued fear recall group that were strong for the non-conditioned group. These results demonstrated unique neuronal and hormonal signalling profiles associated with unconditioned, generalized, and

  13. Novel approach of signal normalization for depth profile of cultural heritage materials

    Science.gov (United States)

    Syvilay, D.; Detalle, V.; Wilkie-Chancellier, N.; Texier, A.; Martinez, L.; Serfaty, S.

    2017-01-01

    The investigation of cultural heritage materials is always complex and specific because unique. Materials are most often heterogeneous and organized in several layers such as mural paintings or corrosion products. The characterization of a complete artwork's stratigraphy is actually one of the questions of science conservation. Indeed, the knowledge of these layers allows completing the history of the work of art and a better understanding of alteration processes in order to set up an appropriate conservation action. The LIBS technique has been employed to study the stratigraphy of an artwork thanks to the ablation laser. However, as we know, atomic information could be insufficient to characterize two materials composed by the same based elements. Therefore, an additional molecular analysis, like Raman spectroscopy; is sometimes necessary for a better identification of the material in particular for organic coatings in cultural heritage. We suggest in this study to use Standard Normal Variate (SNV) as a common normalization for different kinds of spectra (LIBS and Raman spectroscopy) combined with a 3D colour representation for stratigraphic identification of the different layers composing the complex material from artwork. So in this investigation, the SNV method will be applied on LIBS and Raman spectra but also on baseline Raman spectra often considering as nuisance. The aim of this study is to demonstrate the versatility of SNV applied on varied spectra like LIBS, Raman spectra as well as the luminescence background. This original work considers the SNV with a 3D colour representation as a probable new perspective for an easy recognition of a structure layered with a direct overview of the depth profile of the artwork.

  14. Molecular profiling of signalling pathways in formalin-fixed and paraffin-embedded cancer tissues.

    Science.gov (United States)

    Berg, Daniela; Hipp, Susanne; Malinowsky, Katharina; Böllner, Claudia; Becker, Karl-Friedrich

    2010-01-01

    In most hospitals word-wide, histopathological cancer diagnosis is currently based on formalin-fixed and paraffin-embedded (FFPE) tissues. In the last few years new approaches and developments in patient-tailored cancer therapy have raised the need to select more precisely those patients, who will respond to personalised treatments. The most efficient way for optimal therapy and patient selection is probably to provide a tumour-specific protein network portrait prior to treatment. The discovery and characterisation of deregulated signalling molecules (e.g. human epidermal growth factor receptor 2, mitogen-activated protein kinases) are very promising candidates for the identification of new suitable therapy targets and for the selection of those patients who will receive the greatest benefit from individualised treatments. The reverse phase protein array (RPPA) is a promising new technology that allows quick, precise and simultaneous analysis of many components of a network. Importantly it requires only limited amounts of routine clinical material (e.g. FFPE biopsies) and can be used for absolute protein measurements. We and other research groups have described successful protein extraction from routine FFPE tissues. In this manuscript we show how these recent developments might facilitate the implementation of RPPA in clinical trials and routine settings.

  15. Profiling of multiple signal pathway activities by multiplexing antibody and GFP-based translocation assays.

    Science.gov (United States)

    Henriksen, Ulla; Fog, Jacob; Loechel, Frosty; Praestegaard, Morten

    2008-08-01

    Multiplexing of GFP based and immunofluorescence translocation assays enables easy acquisition of multiple readouts from the same cell in a single assay run. Immunofluorescence assays monitor translocation, phosphorylation, and up/down regulation of endogenous proteins. GFP-based assays monitor translocation of stably expressed GFP-fusion proteins. Such assays may be multiplexed along (vertical), across (horizontal), and between (branch) signal pathways. Examples of these strategies are presented: 1) The MK2-GFP assay monitors translocation of MK2-GFP from the nucleus to the cytoplasm in response to stimulation of the p38 pathway. By applying different immunofluorescent assays to the MK2 assay, a multiplexed HCA system is created for deconvolution of p38 pathway activation including assay readouts for MK2, p38, NFkappaB, and c-Jun. 2) A method for evaluating GPCR activation and internalization in a single assay run has been established by multiplexing GFP-based internalization assays with immunofluorescence assays for downstream transducers of GPCR activity: pCREB (cAMP sensor), NFATc1 (Ca(2+) sensor), and ERK (G-protein activation). Activation of the AT1 receptor is given as an example. 3) Cell toxicity readouts can be linked to primary readouts of interest via acquisition of secondary parameters describing cellular morphology. This approach is used to flag cytotoxic compounds and deselect false positives. The ATF6 Redistribution assay is provided as an example. These multiplex strategies provide a unique opportunity to enhance HCA data quality and save time during drug discovery. From a single assay run, several assay readouts are obtained that help the user to deconvolute the mode of action of test compounds.

  16. Detecting and Measuring the Masses of Isolated Black Holes and Neutron Stars through Astrometric Microlensing

    Science.gov (United States)

    Sahu, Kailash

    2013-10-01

    We propose a 3-year program of monitoring of 12 fields in the Galactic bulge, containing a total of 1.5 million stars down to V=28. Our primary aim is to detect microlensing events caused by non-luminous isolated black holes {BHs} and neutron stars {NSs} in the Galactic disk and bulge.The unique capability of HST imaging for microlensing observations is the addition of high-precision astrometry, allowing detection of the astrometric shift of the source during the event. Combined with the lens parallax, provided by the HST event light curve, the astrometric shift provides a direct measurement of the lens mass. We will detect 120 microlensing events, of which 45% will show astrometric deflections, leading to direct determinations of the lens masses. Of these, about 18 lenses are expected to be BHs and 14 of them NSs, along with about 22 events due to main-sequence stars.To date, BH and NS masses have been directly measured only in binaries; no isolated BH has been detected unambiguously within our Galaxy. A survey of the scope proposed here is the only means available at present for measuring the mass function of isolated BHs and NSs, and moreover one that is normalized to that of luminous stars. The results will provide a quantitative estimate of the mass content in the form of stellar remnants in the young Galactic disk and old bulge, and important constraints on SN/GRB explosion mechanisms that produce NSs and BHs.Our data will also be useful for other investigations, including a more accurate determination of the microlensing optical depth, faint variable stars, bulge proper motions and kinematics, and a deep luminosity function of the disk and bulge stars.

  17. ASTROMETRIC POSITIONING OF THE VENEZUELAN SATELLITE VENESAT-1 “PASAVEN”

    OpenAIRE

    Otero, S.; Abad, C; Hernández, R.

    2014-01-01

    En este trabajo informamos de la colaboraci ́n entre dos instituciones venezolanas, la Agencia Bolivariana para o Actividades Espaciales (ABAE) y el Centro de Investigaciones de Astronom ́ (CIDA), con el prop ́sito de ıa o hacer uso de la Astrometr ́ para el seguimiento del sat ́lite geoestacionario venezolano VENESAT1 a partir ıa e de observaciones ́pticas. Para ello, dos peque ̃os observatorios han sido instalados junto a las estaciones o n terrenas de seguimiento que el ABAE posee en Vene...

  18. Data Mining for Dwarf Novae in SDSS, GALEX and Astrometric Catalogues

    CERN Document Server

    Wils, Patrick; Drake, Andrew J; Southworth, John

    2009-01-01

    By cross matching blue objects from SDSS with GALEX and the astrometric catalogues USNO-B1.0, GSC2.3 and CMC14, 64 new dwarf nova candidates with one or more observed outbursts have been identified. 14 of these systems are confirmed as cataclysmic variables through existing and follow-up spectroscopy. A study of the amplitude distribution and an estimate of the outburst frequency of these new dwarf novae and those discovered by the Catalina Real-time Transient Survey (CRTS) indicates that besides systems that are faint because they are farther away, there also exists a population of intrinsically faint dwarf novae with rare outbursts.

  19. The principle of measuring unusual change of underground mass by optical astrometric instrument

    Directory of Open Access Journals (Sweden)

    Wang Jiancheng

    2012-11-01

    In this study, we estimate the deflection angle of the plumb line on a ground site, and give a relation between the angle, abnormal mass and site distance (depth and horizontal distance. Then we derive the abnormality of underground material density using the plumb lines measured at different sites, and study the earthquake gestation, development and occurrence. Using the deflection angles of plumb lines observed at two sites, we give a method to calculate the mass and the center of gravity of underground materials. We also estimate the abnormal masses of latent seismic zones with different energy, using thermodynamic relations, and introduce a new optical astrometric instrument we had developed.

  20. Iterative methods used in overlap astrometric reduction techniques do not always converge

    Science.gov (United States)

    Rapaport, M.; Ducourant, C.; Colin, J.; Le Campion, J. F.

    1993-04-01

    In this paper we prove that the classical Gauss-Seidel type iterative methods used for the solution of the reduced normal equations occurring in overlapping reduction methods of astrometry do not always converge. We exhibit examples of divergence. We then analyze an alternative algorithm proposed by Wang (1985). We prove the consistency of this algorithm and verify that it can be convergent while the Gauss-Seidel method is divergent. We conjecture the convergence of Wang method for the solution of astrometric problems using overlap techniques.

  1. ASTROMETRIC REDUCTION OF GEOSTATIONARY SATELLITES OPTICAL OBSERVATIONS FOR ORBIT DETERMINATION (PASAGE)

    OpenAIRE

    F. J. Montojo; T. López Moratalla; C. Abad; J. L. Muiños

    2008-01-01

    El conocimiento de las efemérides de los satélites geostacionarios es de gran importancia para las agencias de control, tanto para programar maniobras como para comprobar sus resultados. El proyecto PASAGE (Posicionamiento Astrométrico de Satélites Geoestacionarios) tiene como principal objetivo determinar con precisión astrométrica estas efemérides desde telescopios basados en tierra, y la posterior determinación de sus órbitas. Para esta especial aplicación de la astrometría, hemos desarrol...

  2. Signaling transcript profile of the asexual intraerythrocytic development cycle of Plasmodium falciparum induced by melatonin and cAMP

    Science.gov (United States)

    Rozanski, Andrei; Parreira, Kleber S.; Moraes, Miriam S.; Martins, David C.; Hashimoto, Ronaldo F.; Galante, Pedro A.F.; Garcia, Célia R.S.

    2016-01-01

    According to the World Health Organization (WHO), Plasmodium falciparum is the deadliest parasite among all species. This parasite possesses the ability to sense molecules, including melatonin (MEL) and cAMP, and modulate its cell cycle accordingly. MEL synchronizes the development of this malaria parasite by activating several cascades, including the generation of the second messenger cAMP. Therefore, we performed RNA sequencing (RNA-Seq) analysis in P. falciparum erythrocytic stages (ring, trophozoite and schizont) treated with MEL and cAMP. To investigate the expression profile of P. falciparum genes regulated by MEL and cAMP, we performed RNA-Seq analysis in three P. falciparum strains (control, 3D7; protein kinase 7 knockout, PfPK7-; and PfPK7 complement, PfPK7C). In the 3D7 strain, 38 genes were differentially expressed upon MEL treatment; however, none of the genes in the trophozoite (T) stage PfPK7- knockout parasites were differentially expressed upon MEL treatment for 5 hours compared to untreated controls, suggesting that PfPK7 may be involved in the signaling leading to differential gene expression. Moreover, we found that MEL modified the mRNA expression of genes encoding membrane proteins, zinc ion-binding proteins and nucleic acid-binding proteins, which might influence numerous functions in the parasite. The RNA-Seq data following treatment with cAMP show that this molecule modulates different genes throughout the intraerythrocytic cycle, namely, 75, 101 and 141 genes, respectively, in the ring (R), T and schizont (S) stages. Our results highlight P. falciparum's perception of the external milieu through the signaling molecules MEL and cAMP, which are able to drive to changes in gene expression in the parasite. PMID:28050233

  3. Astrometric discovery of GJ 802b : in the Brown Dwarf Oasis?

    Science.gov (United States)

    Pravdo, Steven H.; Shaklan, Stuart B.; Lloyd, James

    2005-01-01

    The Stellar Planet Survey is an ongoing astrometric search for giant planets and brown dwarfs around a sample of 30 M dwarfs. We have discovered several low-mass companions by measuring the motion of our target stars relative to their reference frames. The lowest mass discovery thus far is GJ 802b, a companion to the M5 dwarf GJ 802A. The orbital period is 3.14 +/-0:03 yr, the system mass is 0:214 +/- 0:045 M(circled dot operator), and the semimajor axis is 1:28+/- 0:10 AU or 81 + 6 mas. Imaging observations indicate that GJ 802b is likely to be a brow with the astrometrically determined mass 0:058 +/- 0:021 M(circled dot operator) (1 (sigma) limits). The remaining uncertainty in the orbit is the eccentricity that is now loosely constrained. We dis the system age limits the mass and the prospects of further narrowing the mass range when e is more precisely determined.

  4. A Global Astrometric Solution for Pan-STARRS referenced to ICRF2

    CERN Document Server

    Berghea, C T; Frouard, J; Hennessy, G S; Dorland, B N; Veillette, D R; Dudik, R P; Magnier, E A; Burgett, W S; Chambers, K C; Denneau, L; Flewelling, H; Kaiser, N; Tonry, J L; Wainscoat, R J; Sesar, B

    2016-01-01

    We describe development and application of a Global Astrometric Solution (GAS) to the problem of Pan-STARRS1 (PS1) astrometry. Current PS1 astrometry is based on differential astrometric measurements using 2MASS reference stars, thus PS1 astrometry inherits the errors of the 2MASS catalog. The GAS, based on a single, least squares adjustment to approximately 750k grid stars using over 3000 extragalactic objects as reference objects, avoids this catalog-to-catalog propagation of errors to a great extent. The GAS uses a relatively small number of Quasi-Stellar Objects (QSOs, or distant AGN) with very accurate (<1 mas) radio positions, referenced to the ICRF2. These QSOs provide a hard constraint in the global least squares adjustment. Solving such a system provides absolute astrometry for all the stars simultaneously. The concept is much cleaner than conventional astrometry but is not easy to perform for large catalogs. In this paper we describe our method and its application to Pan-STARRS1 data. We show tha...

  5. A Test of GEMS Astrometric Precision for Exoplanet Detection and Mass Measurement

    Science.gov (United States)

    Ammons, S. Mark; Marois, Christian; Macintosh, Bruce; Konopacky, Quinn; Neichel, Benoit; Galicher, Raphael; Bendek, Eduardo; Guyon, Olivier

    2014-08-01

    Precision astrometry is so far the only mainstream exoplanet detection technique that has yet to find a new planet. The unique capabilities of GeMS and GSAOI may finally be what we have been waiting for: the combination of a large aperture and wide-field AO correction for stable high-resolution wide-field diffraction-limited imaging. As part of this program, we have observed the astrometric calibrator star TYC 7122-00041-1 to demonstrate GeMS' long-term astrometric precision of < 0.4 mas in sparse fields (Ammons et al. 2013). Here, we propose two more epochs on the closest brown dwarf pair at 2 pc, WISE J1049-53 (Luhman 2013), newly discovered with Gemini in 2013 to be the third closest system known. GEMS will in one year obtain the best available projected relative orbits and a < 1% trigonometric distance, enabling precision masses and luminosity measurements for both L/T transition components of WISE 1049-53.

  6. Astrometric Monitoring of the HR 8799 Planets: Orbit Constraints from Self-consistent Measurements

    Science.gov (United States)

    Konopacky, Q. M.; Marois, C.; Macintosh, B. A.; Galicher, R.; Barman, T. S.; Metchev, S. A.; Zuckerman, B.

    2016-08-01

    We present new astrometric measurements from our ongoing monitoring campaign of the HR 8799 directly imaged planetary system. These new data points were obtained with NIRC2 on the W.M. Keck II 10 m telescope between 2009 and 2014. In addition, we present updated astrometry from previously published observations in 2007 and 2008. All data were reduced using the SOSIE algorithm, which accounts for systematic biases present in previously published observations. This allows us to construct a self-consistent data set derived entirely from NIRC2 data alone. From this data set, we detect acceleration for two of the planets (HR 8799b and e) at >3σ. We also assess possible orbital parameters for each of the four planets independently. We find no statistically significant difference in the allowed inclinations of the planets. Fitting the astrometry while forcing coplanarity also returns χ 2 consistent to within 1σ of the best fit values, suggesting that if inclination offsets of ≲20° are present, they are not detectable with current data. Our orbital fits also favor low eccentricities, consistent with predictions from dynamical modeling. We also find period distributions consistent to within 1σ with a 1:2:4:8 resonance between all planets. This analysis demonstrates the importance of minimizing astrometric systematics when fitting for solutions to highly undersampled orbits.

  7. The baseline paradox of optical interferometry. Application to the GRAVITY astrometric error budget

    CERN Document Server

    Lacour, S; Gillessen, S; Pfuhl, O; Woillez, J; Bonnet, H; Perrin, G; Lazareff, B; Rabien, S; Lapeyrere, V; Clenet, Y; Kok, Y

    2014-01-01

    A basic principle of long baseline interferometry is that an optical path difference (OPD) directly translate into an astrometric measurement. In the simplest case, the OPD is equal to the scalar product between the vector linking the two telescopes and the normalized vector pointing toward the star. However, in some circumstances, a too simple approximation interpretation of this scalar product leads to seemingly conflicting results, called here "the baseline paradox". For micro-arcsecond accuracy astrometry, we have to model in full the metrology measurement. It involves a complex system subject to many optical effects: from pure baseline errors to static, quasi-static and high order optical aberrations. The goal of this paper is to present the strategy used by GRAVITY to minimize the biases introduced by these defects. It is possible to give an analytical formula on how the baselines and tip-tilt errors affect the astrometric measurement. This formula depends on the limit-points of three type of baselines:...

  8. Protein profiles reveal diverse responsive signaling pathways in kernels of two maize inbred lines with contrasting drought sensitivity.

    Science.gov (United States)

    Yang, Liming; Jiang, Tingbo; Fountain, Jake C; Scully, Brian T; Lee, Robert D; Kemerait, Robert C; Chen, Sixue; Guo, Baozhu

    2014-01-01

    Drought stress is a major factor that contributes to disease susceptibility and yield loss in agricultural crops. To identify drought responsive proteins and explore metabolic pathways involved in maize tolerance to drought stress, two maize lines (B73 and Lo964) with contrasting drought sensitivity were examined. The treatments of drought and well water were applied at 14 days after pollination (DAP), and protein profiles were investigated in developing kernels (35 DAP) using iTRAQ (isobaric tags for relative and absolute quantitation). Proteomic analysis showed that 70 and 36 proteins were significantly altered in their expression under drought treatments in B73 and Lo964, respectively. The numbers and levels of differentially expressed proteins were generally higher in the sensitive genotype, B73, implying an increased sensitivity to drought given the function of the observed differentially expressed proteins, such as redox homeostasis, cell rescue/defense, hormone regulation and protein biosynthesis and degradation. Lo964 possessed a more stable status with fewer differentially expressed proteins. However, B73 seems to rapidly initiate signaling pathways in response to drought through adjusting diverse defense pathways. These changes in protein expression allow for the production of a drought stress-responsive network in maize kernels.

  9. Protein Profiles Reveal Diverse Responsive Signaling Pathways in Kernels of Two Maize Inbred Lines with Contrasting Drought Sensitivity

    Directory of Open Access Journals (Sweden)

    Liming Yang

    2014-10-01

    Full Text Available Drought stress is a major factor that contributes to disease susceptibility and yield loss in agricultural crops. To identify drought responsive proteins and explore metabolic pathways involved in maize tolerance to drought stress, two maize lines (B73 and Lo964 with contrasting drought sensitivity were examined. The treatments of drought and well water were applied at 14 days after pollination (DAP, and protein profiles were investigated in developing kernels (35 DAP using iTRAQ (isobaric tags for relative and absolute quantitation. Proteomic analysis showed that 70 and 36 proteins were significantly altered in their expression under drought treatments in B73 and Lo964, respectively. The numbers and levels of differentially expressed proteins were generally higher in the sensitive genotype, B73, implying an increased sensitivity to drought given the function of the observed differentially expressed proteins, such as redox homeostasis, cell rescue/defense, hormone regulation and protein biosynthesis and degradation. Lo964 possessed a more stable status with fewer differentially expressed proteins. However, B73 seems to rapidly initiate signaling pathways in response to drought through adjusting diverse defense pathways. These changes in protein expression allow for the production of a drought stress-responsive network in maize kernels.

  10. Metabolic profiling reveals potential metabolic markers associated with Hypoxia Inducible Factor-mediated signalling in hypoxic cancer cells.

    Science.gov (United States)

    Armitage, Emily G; Kotze, Helen L; Allwood, J William; Dunn, Warwick B; Goodacre, Royston; Williams, Kaye J

    2015-10-28

    Hypoxia inducible factors (HIFs) plays an important role in oxygen compromised environments and therefore in tumour survival. In this research, metabolomics has been applied to study HIFs metabolic function in two cell models: mouse hepatocellular carcinoma and human colon carcinoma, whereby the metabolism has been profiled for a range of oxygen potentials. Wild type cells have been compared to cells deficient in HIF signalling to reveal its effect on cellular metabolism under normal oxygen conditions as well as low oxygen, hypoxic and anoxic environments. Characteristic responses to hypoxia that were conserved across both cell models involved the anti-correlation between 2-hydroxyglutarate, 2-oxoglutarate, fructose, hexadecanoic acid, hypotaurine, pyruvate and octadecenoic acid with 4-hydroxyproline, aspartate, cysteine, glutamine, lysine, malate and pyroglutamate. Further to this, network-based correlation analysis revealed HIF specific pathway responses to each oxygen condition that were also conserved between cell models. From this, 4-hydroxyproline was revealed as a regulating hub in low oxygen survival of WT cells while fructose appeared to be in HIF deficient cells. Pathways surrounding these hubs were built from the direct connections of correlated metabolites that look beyond traditional pathways in order to understand the mechanism of HIF response to low oxygen environments.

  11. Flow velocity profiling using acoustic time of flight flow metering based on wide band signals and adaptive beam-forming techniques

    Science.gov (United States)

    Murgan, I.; Candel, I.; Ioana, C.; Digulescu, A.; Bunea, F.; Ciocan, G. D.; Anghel, A.; Vasile, G.

    2016-11-01

    In this paper, we present a novel approach to non-intrusive flow velocity profiling technique using multi-element sensor array and wide-band signal's processing methods. Conventional techniques for the measurements of the flow velocity profiles are usually based on intrusive instruments (current meters, acoustic Doppler profilers, Pitot tubes, etc.) that take punctual velocity readings. Although very efficient, these choices are limited in terms of practical cases of applications especially when non-intrusive measurements techniques are required and/or a spatial accuracy of the velocity profiling is required This is due to factors related to hydraulic machinery down time, the often long time duration needed to explore the entire section area, the frequent cumbersome number of devices that needs to be handled simultaneously, or the impossibility to perform intrusive tests. In the case of non-intrusive flow profiling methods based on acoustic techniques, previous methods concentrated on using a large number of acoustic transducers placed around the measured section. Although feasible, this approach presents several major drawbacks such as a complicated signal timing, transmission, acquisition and recording system, resulting in a relative high cost of operation. In addition, because of the geometrical constraints, a desired number of sensors may not be installed. Recent results in acoustic flow metering based on wide band signals and adaptive beamforming proved that it is possible to achieve flow velocity profiles using less acoustic transducers. In a normal acoustic time of flight path the transducers are both emitters and receivers, sequentially changing their roles. In the new configuration, proposed in this paper, two new receivers are added on each side. Since the beam angles of each acoustic transducer are wide enough the newly added transducers can receive the transmitted signals and additional time of flight estimation can be done. Thus, several flow

  12. Automated Astrometric Analysis of Satellite Observations using Wide-field Imaging

    Science.gov (United States)

    Skuljan, J.; Kay, J.

    2016-09-01

    An observational trial was conducted in the South Island of New Zealand from 24 to 28 February 2015, as a collaborative effort between the United Kingdom and New Zealand in the area of space situational awareness. The aim of the trial was to observe a number of satellites in low Earth orbit using wide-field imaging from two separate locations, in order to determine the space trajectory and compare the measurements with the predictions based on the standard two-line elements. This activity was an initial step in building a space situational awareness capability at the Defence Technology Agency of the New Zealand Defence Force. New Zealand has an important strategic position as the last land mass that many satellites selected for deorbiting pass before entering the Earth's atmosphere over the dedicated disposal area in the South Pacific. A preliminary analysis of the trial data has demonstrated that relatively inexpensive equipment can be used to successfully detect satellites at moderate altitudes. A total of 60 satellite passes were observed over the five nights of observation and about 2600 images were collected. A combination of cooled CCD and standard DSLR cameras were used, with a selection of lenses between 17 mm and 50 mm in focal length, covering a relatively wide field of view of 25 to 60 degrees. The CCD cameras were equipped with custom-made GPS modules to record the time of exposure with a high accuracy of one millisecond, or better. Specialised software has been developed for automated astrometric analysis of the trial data. The astrometric solution is obtained as a two-dimensional least-squares polynomial fit to the measured pixel positions of a large number of stars (typically 1000) detected across the image. The star identification is fully automated and works well for all camera-lens combinations used in the trial. A moderate polynomial degree of 3 to 5 is selected to take into account any image distortions introduced by the lens. A typical RMS

  13. Alternations in genes expression of pathway signaling in esophageal tissue with atresia: results of expression microarray profiling.

    Science.gov (United States)

    Smigiel, R; Lebioda, A; Blaszczyński, M; Korecka, K; Czauderna, P; Korlacki, W; Jakubiak, A; Bednarczyk, D; Maciejewski, H; Wizinska, P; Sasiadek, M M; Patkowski, D

    2015-04-01

    Esophageal atresia (EA) is a congenital defect of the esophagus involving the interruption of the esophagus with or without connection to the trachea (tracheoesophageal fistula [TEF]). EA/TEF may occur as an isolated anomaly, may be part of a complex of congenital defects (syndromic), or may develop within the context of a known syndrome or association. The molecular mechanisms underlying the development of EA are poorly understood. It is supposed that a combination of multigenic factors and epigenetic modification of genes play a role in its etiology. The aim of our work was to assess the human gene expression microarray study in esophageal tissue samples. Total RNA was extracted from 26 lower pouches of esophageal tissue collected during thoracoscopic EA repair in neonates with the isolated (IEA) and the syndromic form (SEA). We identified 787 downregulated and 841 upregulated transcripts between SEA and controls, and about 817 downregulated and 765 upregulated probes between IEA and controls. Fifty percent of these genes showed differential expression specific for either IEA or SEA. Functional pathway analysis revealed substantial enrichment for Wnt and Sonic hedgehog, as well as cytokine and chemokine signaling pathways. Moreover, we performed reverse transcription polymerase chain reaction study in a group of SHH and Wnt pathways genes with differential expression in microarray profiling to confirm the microarray expression results. We verified the altered expression in SFRP2 gene from the Wnt pathway as well as SHH, GLI1, GLI2, and GLI3 from the Sonic hedgehog pathway. The results suggest an important role of these pathways and genes for EA/TEF etiology. © 2014 International Society for Diseases of the Esophagus.

  14. The First U.S. Naval Observatory Robotic Astrometric Telescope Catalog (URAT1)

    CERN Document Server

    Zacharias, Norbert; Subasavage, John; Bredthauer, Greg; Crockett, Chris; DiVittorio, Mike; Ferguson, Erik; Harris, Fred; Harris, Hugh; Henden, Arne; Kilian, Chris; Munn, Jeff; Rafferty, Ted; Rhodes, Al; Schultheiss, Mike; Tilleman, Trudy; Wieder, Gary

    2015-01-01

    URAT1 is an observational, astrometric catalog covering most of the Dec >= -15 deg area and a magnitude range of about R = 3 to 18.5. Accurate positions (typically 10 to 30 mas standard error) are given for over 228 million objects at a mean epoch around 2013.5. For the over 188 million objects matched with the 2MASS point source catalog proper motions (typically 5 to 7 mas/yr std. errors) are provided. These data are supplemented by 2MASS and APASS photometry. Observations, reductions and catalog construction are described together with results from external data verifications. The catalog data are served by CDS, Starsbourg (I/329). There is no DVD release.

  15. Astrometric imaging of crowded stellar fields with only two SIM pointings

    CERN Document Server

    Dalal, N; Dalal, Neal; Griest, Kim

    2001-01-01

    The Space Interferometry Mission (SIM) will observe sources in crowded fields. Recent work has shown that source crowding can induce significant positional errors in SIM's astrometric measurements, even for targets many magnitudes brighter than all other crowding sources. Here we investigate whether the spectral decomposition of the fringe pattern may be used to disentangle the overlapping fringes from multiple blended sources, effectively by performing synthesis imaging with two baselines. We find that spectrally dispersed fringes enable SIM to identify and localize a limited number of field sources quite robustly, thereby removing their effect from SIM astrometry and reducing astrometry errors to near photon noise levels. We simulate SIM measurements of the LMC, and show that (a) SIM astrometry will not be corrupted by blending and (b) extremely precise imaging of mildly crowded fields may be performed using only two orthogonal baseline orientations, allowing microarcsecond positional measurements. We lastl...

  16. Theory of Relativistic Reference Frames for High-Precision Astrometric Space Missions

    CERN Document Server

    Kopeikin, S M

    2000-01-01

    Recent modern space missions deliver invaluable information about origin of our universe, physical processes in the vicinity of black holes and other exotic astrophysical objects, stellar dynamics of our galaxy, etc. On the other hand, space astrometric missions make it possible to determine with unparalleled precision distances to stars and cosmological objects as well as their physical characteristics and positions on the celestial sphere. Permanently growing accuracy of space astronomical observations and the urgent need for adequate data processing algorithms require corresponding development of an adequate theory of reference frames along with unambiguous description of propagation of light rays from a source of light to observer. Such a theory must be based on the Einstein's general relativity and account for numerous relativistic effects both in the solar system and outside of its boundary. The main features of the relativistic theory of reference frames are presented in this work. A hierarchy of the f...

  17. ASTROMETRIC POSITIONING OF THE VENEZUELAN SATELLITE VENESAT-1 “PASAVEN”

    Directory of Open Access Journals (Sweden)

    S. Otero

    2014-01-01

    Full Text Available En este trabajo informamos de la colaboraci ́n entre dos instituciones venezolanas, la Agencia Bolivariana para o Actividades Espaciales (ABAE y el Centro de Investigaciones de Astronom ́ (CIDA, con el prop ́sito de ıa o hacer uso de la Astrometr ́ para el seguimiento del sat ́lite geoestacionario venezolano VENESAT1 a partir ıa e de observaciones ́pticas. Para ello, dos peque ̃os observatorios han sido instalados junto a las estaciones o n terrenas de seguimiento que el ABAE posee en Venezuela, esperando que en un corto espacio de tiempo den los resultados deseados. Asimismo, es nuestro inter ́s extender esta aplicaci ́n a futuros sat ́lites venezolanos, e o e e incluso a la b ́squeda y determinaci ́n de trayectorias de basura espacial geoestacionaria.

  18. Interstellar medium, young stars, and astrometric binaries in Galactic archaeology spectroscopic surveys

    CERN Document Server

    Zwitter, Tomaž; Žerjal, Maruša; Traven, Gregor

    2015-01-01

    Current ongoing stellar spectroscopic surveys (RAVE, GALAH, Gaia-ESO, LAMOST, APOGEE, Gaia) are mostly devoted to studying Galactic archaeology and structure of the Galaxy. But they allow for important auxiliary science: (i) Galactic interstellar medium can be studied in four dimensions (position in space + radial velocity) through weak but numerous diffuse insterstellar bands and atomic absorptions seen in spectra of background stars, (ii) emission spectra which are quite frequent even in field stars can serve as a good indicator of their youth, pointing e.g. to stars recently ejected from young stellar environments, (iii) astrometric solution of the photocenter of a binary to be obtained by Gaia can yield accurate masses when joined by spectroscopic information obtained serendipitously during a survey. These points are illustrated by first results from the first three surveys mentioned above. These hint at the near future: spectroscopic studies of the dynamics of the interstellar medium can identify and qua...

  19. ASTROMETRIC REDUCTION OF GEOSTATIONARY SATELLITES OPTICAL OBSERVATIONS FOR ORBIT DETERMINATION (PASAGE

    Directory of Open Access Journals (Sweden)

    F. J. Montojo

    2008-01-01

    Full Text Available El conocimiento de las efemérides de los satélites geostacionarios es de gran importancia para las agencias de control, tanto para programar maniobras como para comprobar sus resultados. El proyecto PASAGE (Posicionamiento Astrométrico de Satélites Geoestacionarios tiene como principal objetivo determinar con precisión astrométrica estas efemérides desde telescopios basados en tierra, y la posterior determinación de sus órbitas. Para esta especial aplicación de la astrometría, hemos desarrollado técnicas y algoritmos especiales que nos permiten obtener elas posiciones aparentes topocéntricas de los satélites geostacionarios con precisiones de décimas de segundo de arco, incluso desde lugares con alta contaminación lumínica.

  20. Interstellar Medium, Young Stars, and Astrometric Binaries in Galactic Archaeology Spectroscopic Surveys

    Science.gov (United States)

    Zwitter, T.; Kos, J.; Žerjal, M.; Traven, G.

    2016-10-01

    Current ongoing stellar spectroscopic surveys (RAVE, GALAH, Gaia-ESO, LAMOST, APOGEE, Gaia) are mostly devoted to studying Galactic archaeology and the structure of the Galaxy. But they allow also for important auxiliary science: (i) the Galactic interstellar medium can be studied in four dimensions (position in space plus radial velocity) through weak but numerous diffuse interstellar bands and atomic absorptions seen in spectra of background stars, (ii) emission spectra which are quite frequent even in field stars can serve as a good indicator of their youth, pointing e.g. to stars recently ejected from young stellar environments, (iii) an astrometric solution of the photocenter of a binary to be obtained by Gaia can yield accurate masses when joined by spectroscopic information obtained serendipitously during a survey. These points are illustrated by first results from the first three surveys mentioned above. These hint at the near future: spectroscopic studies of the dynamics of the interstellar medium can identify and quantify Galactic fountains which may sustain star formation in the disk by entraining fresh gas from the halo; RAVE already provided a list of ˜ 14,000 field stars with chromospheric emission in Ca II lines, to be supplemented by many more observations by Gaia in the same band, and by GALAH and Gaia-ESO observations of Balmer lines; several millions of astrometric binaries with periods up to a few years which are being observed by Gaia can yield accurate masses when supplemented with measurements from only a few high-quality ground based spectra.

  1. A Model for Astrometric Detection and Characterization of Multi-Exoplanet Systems

    Science.gov (United States)

    April Thompson, Maggie; Spergel, David N.

    2017-01-01

    In this thesis, we develop an approximate linear model of stellar motion in multi- planet systems as an aid to observers using the astrometric method to detect and characterize exoplanets. Recent and near-term advances in satellite and ground-based instruments are on the threshold of achieving sufficient (~10 micro-arcsecond) angular accuracies to allow astronomers to measure and analyze the transverse mo- tion of stars about the common barycenter in single- and multi-planet systems due to the gravitational influence of companion planets. Given the emerging statistics of extrasolar planetary systems and the long observation periods required to assess exoplanet influences, astronomers should find an approximate technique for preliminary estimates of multiple planet numbers, masses and orbital parameters useful in determining the most likely stellar systems for follow-up studies. In this paper, we briefly review the history of astrometry and discuss its advantages and limitations in exoplanet research. In addition, we define the principal astrometric signature and describe the main variables affecting it, highlighting astrometry’s complementary role to radial velocity and photometric transit exoplanet detection techniques. We develop and test a Python computer code using actual data and projections of the Sun’s motion due to the influence of the four gas giants in the solar system. We then apply this model to over 50 hypothetical massive two- and three-exoplanet systems to discover useful general patterns by employing a heuristic examination of key aspects of the host star’s motion over long observation intervals. Finally, we modify the code by incorporating an inverse least-squares fit program to assess its efficiency in identifying the main characteristics of multi-planet systems based on observational records over 5-, 10- and 20-year periods for a variety of actual and hypothetical exoplanetary systems. We also explore the method’s sensitivity to

  2. Expression profiling of cumulus cells reveals functional changes during ovulation and central roles of prostaglandin EP2 receptor in cAMP signaling.

    Science.gov (United States)

    Tamba, Shigero; Yodoi, Rieko; Morimoto, Kazushi; Inazumi, Tomoaki; Sukeno, Mamiko; Segi-Nishida, Eri; Okuno, Yasushi; Tsujimoto, Gozoh; Narumiya, Shuh; Sugimoto, Yukihiko

    2010-06-01

    To understand the role of prostaglandin (PG) receptor EP2 (Ptger2) signaling in ovulation and fertilization, we investigated time-dependent expression profiles in wild-type (WT) and Ptger2(-/-) cumuli before and after ovulation by using microarrays. We prepared cumulus cells from mice just before and 3, 9 and 14 h after human chorionic gonadotropin injection. Key genes including cAMP-related and epidermal growth factor (EGF) genes, as well as extracellular matrix- (ECM-) related and chemokine genes were up-regulated in WT cumuli at 3 h and 14 h, respectively. Ptger2 deficiency differently affected the expression of many of the key genes at 3 h and 14 h. These results indicate that the gene expression profile of cumulus cells greatly differs before and after ovulation, and in each situation, PGE(2)-EP2 signaling plays a critical role in cAMP-regulated gene expression in the cumulus cells under physiological conditions.

  3. Expression profiling and functional analysis reveals that TOR is a key player in regulating photosynthesis and phytohormone signaling pathways in Arabidopsis.

    Science.gov (United States)

    Dong, Pan; Xiong, Fangjie; Que, Yumei; Wang, Kai; Yu, Lihua; Li, Zhengguo; Ren, Maozhi

    2015-01-01

    Target of rapamycin (TOR) acts as a master regulator to control cell growth by integrating nutrient, energy, and growth factors in all eukaryotic species. TOR plays an evolutionarily conserved role in regulating the transcription of genes associated with anabolic and catabolic processes in Arabidopsis, but little is known about the functions of TOR in photosynthesis and phytohormone signaling, which are unique features of plants. In this study, AZD8055 (AZD) was screened as the strongest active-site TOR inhibitor (asTORi) in Arabidopsis compared with TORIN1 and KU63794 (KU). Gene expression profiles were evaluated using RNA-seq after treating Arabidopsis seedlings with AZD. More than three-fold differentially expressed genes (DEGs) were identified in AZD-treated plants relative to rapamycin-treated plants in previous studies. Most of the DEGs and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways involved in cell wall elongation, ribosome biogenesis, and cell autophagy were common to both AZD- and rapamycin-treated samples, but AZD displayed much broader and more efficient inhibition of TOR compared with rapamycin. Importantly, the suppression of TOR by AZD resulted in remodeling of the expression profile of the genes associated with photosynthesis and various phytohormones, indicating that TOR plays a crucial role in modulating photosynthesis and phytohormone signaling in Arabidopsis. These newly identified DEGs expand the understanding of TOR signaling in plants. This study elucidates the novel functions of TOR in photosynthesis and phytohormone signaling and provides a platform to study the downstream targets of TOR in Arabidopsis.

  4. Expression profiling analysis: Uncoupling protein 2 deficiency improves hepatic glucose, lipid profiles and insulin sensitivity in high-fat diet-fed mice by modulating expression of genes in peroxisome proliferator-activated receptor signaling pathway.

    Science.gov (United States)

    Zhou, Mei-Cen; Yu, Ping; Sun, Qi; Li, Yu-Xiu

    2016-03-01

    Uncoupling protein 2 (UCP2), which was an important mitochondrial inner membrane protein associated with glucose and lipid metabolism, widely expresses in all kinds of tissues including hepatocytes. The present study aimed to explore the impact of UCP2 deficiency on glucose and lipid metabolism, insulin sensitivity and its effect on the liver-associated signaling pathway by expression profiling analysis. Four-week-old male UCP2-/- mice and UCP2+/+ mice were randomly assigned to four groups: UCP2-/- on a high-fat diet, UCP2-/- on a normal chow diet, UCP2+/+ on a high-fat diet and UCP2+/+ on a normal chow diet. The differentially expressed genes in the four groups on the 16th week were identified by Affymetrix gene array. The results of intraperitoneal glucose tolerance test and insulin tolerance showed that blood glucose and β-cell function were improved in the UCP2-/- group on high-fat diet. Enhanced insulin sensitivity was observed in the UCP2-/- group. The differentially expressed genes were mapped to 23 pathways (P signaling pathway' (P = 3.19 × 10(-11)), because it is closely associated with the regulation of glucose and lipid profiles. In the PPAR signaling pathway, seven genes (PPARγ, Dbi, Acsl3, Lpl, Me1, Scd1, Fads2) in the UCP2-/- mice were significantly upregulated. The present study used gene arrays to show that activity of the PPAR signaling pathway involved in the improvement of glucose and lipid metabolism in the liver of UCP2-deficient mice on a long-term high-fat diet. The upregulation of genes in the PPAR signaling pathway could explain our finding that UCP2 deficiency ameliorated insulin sensitivity. The manipulation of UCP2 protein expression could represent a new strategy for the prevention and treatment of diabetes.

  5. Verification of the astrometric performance of the Korean VLBI network, using comparative SFPR studies with the VLBA AT 14/7 mm

    Energy Technology Data Exchange (ETDEWEB)

    Rioja, María J.; Dodson, Richard; Jung, TaeHyun; Sohn, Bong Won; Byun, Do-Young; Cho, Se-Hyung; Lee, Sang-Sung; Kim, Jongsoo; Kim, Kee-Tae; Oh, Chung Sik; Han, Seog-Tae; Je, Do-Heung; Chung, Moon-Hee; Wi, Seog-Oh; Kang, Jiman; Lee, Jung-Won; Chung, Hyunsoo; Kim, Hyo Ryoung; Kim, Hyun-Goo [Korea Astronomy and Space Science Institute, Daedeokdae-ro 776, Yuseong-gu, Daejeon 305-348 (Korea, Republic of); Agudo, Iván, E-mail: maria.rioja@icrar.org [Joint Institute for VLBI in Europe, Postbus 2, NL-7990 AA Dwingeloo (Netherlands); and others

    2014-11-01

    The Korean VLBI Network (KVN) is a new millimeter VLBI dedicated array with the capability to simultaneously observe at multiple frequencies, up to 129 GHz. The innovative multi-channel receivers present significant benefits for astrometric measurements in the frequency domain. The aim of this work is to verify the astrometric performance of the KVN using a comparative study with the VLBA, a well-established instrument. For that purpose, we carried out nearly contemporaneous observations with the KVN and the VLBA, at 14/7 mm, in 2013 April. The KVN observations consisted of simultaneous dual frequency observations, while the VLBA used fast frequency switching observations. We used the Source Frequency Phase Referencing technique for the observational and analysis strategy. We find that having simultaneous observations results in superior compensation for all atmospheric terms in the observables, in addition to offering other significant benefits for astrometric analysis. We have compared the KVN astrometry measurements to those from the VLBA. We find that the structure blending effects introduce dominant systematic astrometric shifts, and these need to be taken into account. We have tested multiple analytical routes to characterize the impact of the low-resolution effects for extended sources in the astrometric measurements. The results from the analysis of the KVN and full VLBA data sets agree within 2σ of the thermal error estimate. We interpret the discrepancy as arising from the different resolutions. We find that the KVN provides astrometric results with excellent agreement, within 1σ, when compared to a VLBA configuration that has a similar resolution. Therefore, this comparative study verifies the astrometric performance of the KVN using SFPR at 14/7 mm, and validates the KVN as an astrometric instrument.

  6. Astrometric Detection of Terrestrial Planets in the Habitable Zones of Nearby Stars with SIM PlanetQuest

    CERN Document Server

    Catanzarite, J; Tanner, A; Unwin, S; Yu, J; Catanzarite, Joseph; Shao, Michael; Tanner, Angelle; Unwin, Stephen; Yu, Jeffrey

    2006-01-01

    SIM PlanetQuest (Space Interferometry Mission) is a space-borne Michelson interferometer for precision stellar astrometry, with a nine meter baseline, currently slated for launch in 2015. One of the principal science goals is the astrometric detection and orbit characterization of terrestrial planets in the habitable zones of nearby stars. Differential astrometry of the target star against a set of reference stars lying within a degree will allow measurement of the target star's reflex motion with astrometric accuracy of 1 micro-arcsecond in a single measurement. We assess SIM's capability for detection (as opposed to characterization by orbit determination) of terrestrial planets in the habitable zones of nearby solar-type stars. We compare SIM's performance on target lists optimized for the SIM and Terrestrial Planet Finder Coronograph (TPF-C) missions. Performance is quantified by three metrics: minimum detectable planet mass, number and mass distribution of detected planets, and completeness of detections...

  7. The ESPRI project: astrometric exoplanet search with PRIMA I. Instrument description and performance of first light observations

    CERN Document Server

    Sahlmann, J; Queloz, D; Quirrenbach, A; Elias, N M; Launhardt, R; Pepe, F; Reffert, S; Segransan, D; Setiawan, J; Abuter, R; Andolfato, L; Bizenberger, P; Baumeister, H; Chazelas, B; Delplancke, F; Derie, F; Di Lieto, N; Duc, T P; Fleury, M; Graser, U; Kaminski, A; Koehler, R; Leveque, S; Maire, C; Megevand, D; Merand, A; Michellod, Y; Moresmau, J -M; Mohler, M; Mueller, A; Muellhaupt, P; Naranjo, V; Sache, L; Salvade, Y; Schmid, C; Schuhler, N; Schulze-Hartung, T; Sosnowska, D; Tubbs, B; van Belle, G T; Wagner, K; Weber, L; Zago, L; Zimmerman, N

    2012-01-01

    The ESPRI project relies on the astrometric capabilities offered by the PRIMA facility of the Very Large Telescope Interferometer for the discovery and study of planetary systems. Our survey consists of obtaining high-precision astrometry for a large sample of stars over several years and to detect their barycentric motions due to orbiting planets. We present the operation principle, the instrument's implementation, and the results of a first series of test observations. A comprehensive overview of the instrument infrastructure is given and the observation strategy for dual-field relative astrometry is presented. The differential delay lines, a key component of the PRIMA facility which was delivered by the ESPRI consortium, are described and their performance within the facility is discussed. Observations of bright visual binaries are used to test the observation procedures and to establish the instrument's astrometric precision and accuracy. The data reduction strategy for astrometry and the necessary correc...

  8. Astrometric and photometric monitoring of GQ Lup and its sub-stellar companion

    CERN Document Server

    Neuhaeuser, Ralph; Seifahrt, Andreas; Schmidt, Tobias; Vogt, Nikolaus

    2008-01-01

    Neuhaeuser et al. (2005) presented direct imaging evidence for a sub-stellar companion to the young T Tauri star GQ Lup. Common proper motion was highly significant, but no orbital motion was detected. Faint luminosity, low gravity, and a late-M/early-L spectral type indicated that the companion is either a planet or a brown dwarf. We have monitored GQ Lup and its companion in order to detect orbital and parallactic motion and variability in its brightness. We also search for closer and fainter companions. We have taken six more images with the VLT Adaptive Optics instrument NACO from May 2005 to Feb 2007, always with the same calibration binary from Hipparcos for both astrometric and photometric calibration. By adding up all the images taken so far, we search for additional companions. The position of GQ Lup A and its companion compared to a nearby non-moving background object varies as expected for parallactic motion by about one pixel (2 \\pi with parallax \\pi). We could not find evidence for variability of...

  9. Double-blind test program for astrometric planet detection with Gaia

    CERN Document Server

    Casertano, S; Sozzetti, A; Spagna, A; Jancart, S; Morbidelli, R; Pannunzio, R; Pourbaix, D; Queloz, D

    2008-01-01

    We use detailed simulations of the Gaia observations of synthetic planetary systems and develop and utilize independent software codes in double-blind mode to analyze the data, including statistical tools for planet detection and different algorithms for single and multiple Keplerian orbit fitting that use no a priori knowledge of the true orbital parameters of the systems. 1) Planets with astrometric signatures $\\alpha\\simeq 3$ times the single-measurement error $\\sigma_\\psi$ and period $P\\leq 5$ yr can be detected reliably, with a very small number of false positives. 2) At twice the detection limit, uncertainties in orbital parameters and masses are typically $15%-20%$. 3) Over 70% of two-planet systems with well-separated periods in the range $0.2\\leq P\\leq 9$ yr, $2\\leq\\alpha/\\sigma_\\psi\\leq 50$, and eccentricity $e\\leq 0.6$ are correctly identified. 4) Favorable orbital configurations have orbital elements measured to better than 10% accuracy $> 90%$ of the time, and the value of the mutual inclination ...

  10. The Astrometric-Spectroscopic Binary System HIP 50796: An Overmassive Companion

    CERN Document Server

    Torres, G

    2005-01-01

    We report spectroscopic observations of the star HIP 50796, previously considered (but later rejected) as a candidate member of the TW Hya association. Our measurements reveal it to be a single-lined binary with an orbital period of 570 days and an eccentricity of e = 0.61. The astrometric signature of this orbit was previously detected by the HIPPARCOS satellite in the form of curvature in the proper motion components, although the period was unknown at the time. By combining our radial velocity measurements with the HIPPARCOS intermediate data (abscissae residuals) we are able to derive the full three-dimensional orbit, and determine the dynamical mass of the unseen companion as well as a revised trigonometric parallax that accounts for the orbital motion. Given our primary mass estimate of 0.73 solar masses (mid-K dwarf), the companion mass is determined to be 0.89 solar masses, or about 20% larger than the primary. The likely explanation for the larger mass without any apparent contribution to the light i...

  11. Astrometric observations of Phobos and Deimos during the 1971 opposition of Mars

    CERN Document Server

    Robert, V; Pascu, D; Arlot, J -E; De Cuyper, J -P; Dehant, V; Thuillot, W

    2015-01-01

    Accurate positional measurements of planets and satellites are used to improve our knowledge of their dynamics and to infer the accuracy of planet and satellite ephemerides. In the framework of the FP7 ESPaCE project, we provide the positions of Mars, Phobos, and Deimos taken with the U.S. Naval Observatory 26-inch refractor during the 1971 opposition of the planet. These plates were measured with the digitizer of the Royal Observatory of Belgium and reduced through an optimal process that includes image, instrumental, and spherical corrections to provide the most accurate data. We compared the observed positions of the planet Mars and its satellites with the theoretical positions from INPOP10 and DE430 planetary ephemerides, and from NOE and MAR097 satellite ephemerides. The rms residuals in RA and Dec. of one position is less than 60 mas, or about 20 km at Mars. This accuracy is comparable to the most recent CCD observations. Moreover, it shows that astrometric data derived from photographic plates can comp...

  12. Astrometric Monitoring of the HR 8799 Planets: Orbit Constraints from Self-Consistent Measurements

    CERN Document Server

    Konopacky, Q M; Macintosh, B A; Galicher, R; Barman, T S; Metchev, S A; Zuckerman, B

    2016-01-01

    We present new astrometric measurements from our ongoing monitoring campaign of the HR 8799 directly imaged planetary system. These new data points were obtained with NIRC2 on the W.M. Keck II 10 meter telescope between 2009 and 2014. In addition, we present updated astrometry from previously published observations in 2007 and 2008. All data were reduced using the SOSIE algorithm, which accounts for systematic biases present in previously published observations. This allows us to construct a self-consistent data set derived entirely from NIRC2 data alone. From this dataset, we detect acceleration for two of the planets (HR 8799b and e) at $>$3$\\sigma$. We also assess possible orbital parameters for each of the four planets independently. We find no statistically significant difference in the allowed inclinations of the planets. Fitting the astrometry while forcing coplanarity also returns $\\chi^2$ consistent to within 1$\\sigma$ of the best fit values, suggesting that if inclination offsets of $\\lesssim$20$^{o...

  13. First experimental results of very high accuracy centroiding measurements for the neat astrometric mission

    CERN Document Server

    Crouzier, A; Preis, O; Henault, F; Kern, P; Martin, G; Feautrier, P; Stadler, E; Lafrasse, S; Behar, E; Saint-Pe, M; Dupont, J; Potin, S; Cara, C; Lagage, P O; Leger, A; LeDuigou, J M; Shao, M; Goullioud, R

    2013-01-01

    NEAT is an astrometric mission proposed to ESA with the objectives of detecting Earth-like exoplanets in the habitable zone of nearby solar-type stars. NEAT requires the capability to measure stellar centroids at the precision of 5e-6 pixel. Current state-of-the-art methods for centroid estimation have reached a precision of about 2e-5 pixel at two times Nyquist sampling, this was shown at the JPL by the VESTA experiment. A metrology system was used to calibrate intra and inter pixel quantum efficiency variations in order to correct pixelation errors. The European part of the NEAT consortium is building a testbed in vacuum in order to achieve 5e-6 pixel precision for the centroid estimation. The goal is to provide a proof of concept for the precision requirement of the NEAT spacecraft. In this paper we present the metrology and the pseudo stellar sources sub-systems, we present a performance model and an error budget of the experiment and we report the present status of the demonstration. Finally we also pres...

  14. The Second Post-Newtonian Light Propagation and Its Astrometric Measurement in the SOLAR SYSTEM

    CERN Document Server

    Deng, Xue-Mei

    2015-01-01

    The relativistic theories of light propagation are generalized by introducing two new parameters $\\varsigma$ and $\\eta$ in the second post-Newtonian (2PN) order, in addition to the parameterized post-Newtonian parameters $\\gamma$ and $\\beta$. This new 2PN parameterized (2PPN) formalism includes the non-stationary gravitational fields and the influences of all kinds of relativistic effects. The multipolar components of gravitating bodies are taken into account as well at the first post-Newtonian order. The equations of motion and their solutions of this 2PPN light propagation problem are obtained. Started from the definition of a measurable quantity, a gauge-invariant angle between the directions of two incoming photons for a differential measurement in astrometric observation is discussed and its formula is derived. For a precision level of a few microacrsecond ($\\mu$as) for space astrometry missions in the near future, we further consider a model of angular measurement, LATOR-like missions. In this case, all...

  15. Habitable Worlds Around M Dwarf Stars: The CAPSCam Astrometric Planet Search

    Science.gov (United States)

    Boss, Alan P.; Weinberger, Alycia J.; Anglada-Escudé, Guillem; Thompson, Ian B.; Brahm, Rafael

    2014-04-01

    M dwarf stars are attractive targets in the search for habitable worlds as a result of their relative abundance and proximity, making them likely targets for future direct detection efforts. Hot super-Earths as well as gas giants have already been detected around a number of early M dwarfs, and the former appear to be the high-mass end of the population of rocky, terrestrial exoplanets. The Carnegie Astrometric Planet Search (CAPS) program has been underway since March 2007, searching ~ 100 nearby late M, L, and T dwarfs for gas giant planets on orbits wide enough for habitable worlds to orbit interior to them. The CAPSCam-N camera on the 2.5-m du Pont telescope at the Las Campanas Observatory has demonstrated the ability to detect planets as low in mass as Saturn orbiting at several AU around late M dwarfs within 15 pc. Over the next decade, the CAPS program will provide new constraints on the planetary census around late M dwarf stars, and hence on the suitability of these nearby planetary systems for supporting life.

  16. THE APPLICATION OF MULTIVIEW METHODS FOR HIGH-PRECISION ASTROMETRIC SPACE VLBI AT LOW FREQUENCIES

    Energy Technology Data Exchange (ETDEWEB)

    Dodson, R.; Rioja, M.; Imai, H. [International Centre for Radio Astronomy Research, M468, University of Western Australia, 35 Stirling Hwy, Crawley, Western Australia 6009 (Australia); Asaki, Y. [Institute of Space and Astronautical Science, 3-1-1 Yoshinodai, Chuou, Sagamihara, Kanagawa 252-5210 (Japan); Hong, X.-Y.; Shen, Z., E-mail: richard.dodson@icrar.org [Shanghai Astronomical Observatory, CAS, 200030 Shanghai (China)

    2013-06-15

    High-precision astrometric space very long baseline interferometry (S-VLBI) at the low end of the conventional frequency range, i.e., 20 cm, is a requirement for a number of high-priority science goals. These are headlined by obtaining trigonometric parallax distances to pulsars in pulsar-black hole pairs and OH masers anywhere in the Milky Way and the Magellanic Clouds. We propose a solution for the most difficult technical problems in S-VLBI by the MultiView approach where multiple sources, separated by several degrees on the sky, are observed simultaneously. We simulated a number of challenging S-VLBI configurations, with orbit errors up to 8 m in size and with ionospheric atmospheres consistent with poor conditions. In these simulations we performed MultiView analysis to achieve the required science goals. This approach removes the need for beam switching requiring a Control Moment Gyro, and the space and ground infrastructure required for high-quality orbit reconstruction of a space-based radio telescope. This will dramatically reduce the complexity of S-VLBI missions which implement the phase-referencing technique.

  17. The application of MultiView Methods for High Precision Astrometric Space VLBI at Low Frequencies

    CERN Document Server

    Dodson, R; Asaki, Y; Imai, H; Hong, X -Y; Shen, Z

    2013-01-01

    High precision astrometric Space Very Long Baseline Interferometry (S-VLBI) at the low end of the conventional frequency range, i.e. 20cm, is a requirement for a number of high priority science goals. These are headlined by obtaining trigonometric parallax distances to pulsars in Pulsar--Black Hole pairs and OH masers anywhere in the Milky Way Galaxy and the Magellanic Clouds. We propose a solution for the most difficult technical problems in S-VLBI by the MultiView approach where multiple sources, separated by several degrees on the sky, are observed simultaneously. We simulated a number of challenging S-VLBI configurations, with orbit errors up to 8m in size and with ionospheric atmospheres consistant with poor conditions. In these simulations we performed MultiView analysis to achieve the required science goals. This approach removes the need for beam switching requiring a Control Moment Gyro, and the space and ground infrastructure required for high quality orbit reconstruction of a space-based radio tele...

  18. Global gene expression profiling in infants with acute respiratory syncytial virus broncholitis demonstrates systemic activation of interferon signaling networks

    Science.gov (United States)

    Respiratory syncytial virus (RSV) is a leading cause of pediatric lower respiratory tract infections and has a high impact on pediatric emergency department utilization. Variation in host response may influence the pathogenesis and disease severity. We evaluated global gene expression profiles to be...

  19. Gene expression profiling of peri-implant healing of PLGA-Li+ implants suggests an activated Wnt signaling pathway in vivo.

    Directory of Open Access Journals (Sweden)

    Anna Thorfve

    Full Text Available Bone development and regeneration is associated with the Wnt signaling pathway that, according to literature, can be modulated by lithium ions (Li+. The aim of this study was to evaluate the gene expression profile during peri-implant healing of poly(lactic-co-glycolic acid (PLGA implants with incorporated Li+, while PLGA without Li+ was used as control, and a special attention was then paid to the Wnt signaling pathway. The implants were inserted in rat tibia for 7 or 28 days and the gene expression profile was investigated using a genome-wide microarray analysis. The results were verified by qPCR and immunohistochemistry. Histomorphometry was used to evaluate the possible effect of Li+ on bone regeneration. The microarray analysis revealed a large number of significantly differentially regulated genes over time within the two implant groups. The Wnt signaling pathway was significantly affected by Li+, with approximately 34% of all Wnt-related markers regulated over time, compared to 22% for non-Li+ containing (control; Ctrl implants. Functional cluster analysis indicated skeletal system morphogenesis, cartilage development and condensation as related to Li+. The downstream Wnt target gene, FOSL1, and the extracellular protein-encoding gene, ASPN, were significantly upregulated by Li+ compared with Ctrl. The presence of β-catenin, FOSL1 and ASPN positive cells was confirmed around implants of both groups. Interestingly, a significantly reduced bone area was observed over time around both implant groups. The presence of periostin and calcitonin receptor-positive cells was observed at both time points. This study is to the best of the authors' knowledge the first report evaluating the effect of a local release of Li+ from PLGA at the fracture site. The present study shows that during the current time frame and with the present dose of Li+ in PLGA implants, Li+ is not an enhancer of early bone growth, although it affects the Wnt signaling pathway.

  20. Expression profiling and functional analysis reveals that TOR is a key player in regulating photosynthesis and phytohormone signaling pathways in Arabidopsis

    Directory of Open Access Journals (Sweden)

    Pan eDong

    2015-09-01

    Full Text Available Target of rapamycin (TOR acts as a master regulator to control cell growth by integrating nutrient, energy, and growth factors in all eukaryotic species. TOR plays an evolutionarily conserved role in regulating the transcription of genes associated with anabolic and catabolic processes in Arabidopsis, but little is known about the functions of TOR in photosynthesis and phytohormone signaling, which are unique features of plants. In this study, AZD8055 (AZD was screened as the strongest active-site TOR inhibitor (asTORi in Arabidopsis compared with TORIN1 and KU63794 (KU. Gene expression profiles were evaluated using RNA-seq after treating Arabidopsis seedlings with AZD. More than 3-fold differentially expressed genes (DEGs were identified in AZD-treated plants relative to rapamycin-treated plants in previous studies. Most of the DEGs and Kyoto Encyclopedia of Genes and Genomes (KEGG pathways involved in cell wall elongation, ribosome biogenesis and cell autophagy were common to both AZD- and rapamycin-treated samples, but AZD displayed much broader and more efficient inhibition of TOR compared with rapamycin. Importantly, the suppression of TOR by AZD resulted in remodeling of the expression profile of the genes associated with photosynthesis and various phytohormones, indicating that TOR plays a crucial role in modulating photosynthesis and phytohormone signaling in Arabidopsis. These newly identified DEGs expand the understanding of TOR signaling in plants. This study elucidates the novel functions of TOR in photosynthesis and phytohormone signaling and provides a platform to study the downstream targets of TOR in Arabidopsis.

  1. Modeling of very low frequency (VLF radio wave signal profile due to solar flares using the GEANT4 Monte Carlo simulation coupled with ionospheric chemistry

    Directory of Open Access Journals (Sweden)

    S. Palit

    2013-09-01

    Full Text Available X-ray photons emitted during solar flares cause ionization in the lower ionosphere (~60 to 100 km in excess of what is expected to occur due to a quiet sun. Very low frequency (VLF radio wave signals reflected from the D-region of the ionosphere are affected by this excess ionization. In this paper, we reproduce the deviation in VLF signal strength during solar flares by numerical modeling. We use GEANT4 Monte Carlo simulation code to compute the rate of ionization due to a M-class flare and a X-class flare. The output of the simulation is then used in a simplified ionospheric chemistry model to calculate the time variation of electron density at different altitudes in the D-region of the ionosphere. The resulting electron density variation profile is then self-consistently used in the LWPC code to obtain the time variation of the change in VLF signal. We did the modeling of the VLF signal along the NWC (Australia to IERC/ICSP (India propagation path and compared the results with observations. The agreement is found to be very satisfactory.

  2. Spatial Phosphoprotein Profiling Reveals a Compartmentalized Extracellular Signal-regulated Kinase Switch Governing Neurite Growth and Retraction

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Yingchun; Yang, Feng; Fu, Yi; Huang, Xiahe; Wang, Wei; Jiang, Xining; Gritsenko, Marina A.; Zhao, Rui; Monroe, Matthew E.; Pertz, Olivier C.; Purvine, Samuel O.; Orton, Daniel J.; Jacobs, Jon M.; Camp, David G.; Smith, Richard D.; Klemke, Richard L.

    2011-05-20

    Abstract - Brain development and spinal cord regeneration require neurite sprouting and growth cone navigation in response to extension and collapsing factors present in the extracellular environment. These external guidance cues control neurite growth cone extension and retraction processes through intracellular protein phosphorylation of numerous cytoskeletal, adhesion, and polarity complex signaling proteins. However, the complex kinase/substrate signaling networks that mediate neuritogenesis have not been investigated. Here, we compare the neurite phosphoproteome under growth and retraction conditions using neurite purification methodology combined with mass spectrometry. More than 4000 non-redundant phosphorylation sites from 1883 proteins have been annotated and mapped to signaling pathways that control kinase/phosphatase networks, cytoskeleton remodeling, and axon/dendrite specification. Comprehensive informatics and functional studies revealed a compartmentalized ERK activation/deactivation cytoskeletal switch that governs neurite growth and retraction, respectively. Our findings provide the first system-wide analysis of the phosphoprotein signaling networks that enable neurite growth and retraction and reveal an important molecular switch that governs neuritogenesis.

  3. Spatial Phosphoprotein Profiling Reveals a Compartmentalized Extracellular Signal-regulated Kinase Switch Governing Neurite Growth and Retraction*

    Science.gov (United States)

    Wang, Yingchun; Yang, Feng; Fu, Yi; Huang, Xiahe; Wang, Wei; Jiang, Xinning; Gritsenko, Marina A.; Zhao, Rui; Monore, Matthew E.; Pertz, Olivier C.; Purvine, Samuel O.; Orton, Daniel J.; Jacobs, Jon M.; Camp, David G.; Smith, Richard D.; Klemke, Richard L.

    2011-01-01

    Brain development and spinal cord regeneration require neurite sprouting and growth cone navigation in response to extension and collapsing factors present in the extracellular environment. These external guidance cues control neurite growth cone extension and retraction processes through intracellular protein phosphorylation of numerous cytoskeletal, adhesion, and polarity complex signaling proteins. However, the complex kinase/substrate signaling networks that mediate neuritogenesis have not been investigated. Here, we compare the neurite phosphoproteome under growth and retraction conditions using neurite purification methodology combined with mass spectrometry. More than 4000 non-redundant phosphorylation sites from 1883 proteins have been annotated and mapped to signaling pathways that control kinase/phosphatase networks, cytoskeleton remodeling, and axon/dendrite specification. Comprehensive informatics and functional studies revealed a compartmentalized ERK activation/deactivation cytoskeletal switch that governs neurite growth and retraction, respectively. Our findings provide the first system-wide analysis of the phosphoprotein signaling networks that enable neurite growth and retraction and reveal an important molecular switch that governs neuritogenesis. PMID:21454597

  4. Spatial phosphoprotein profiling reveals a compartmentalized extracellular signal-regulated kinase switch governing neurite growth and retraction.

    Science.gov (United States)

    Wang, Yingchun; Yang, Feng; Fu, Yi; Huang, Xiahe; Wang, Wei; Jiang, Xinning; Gritsenko, Marina A; Zhao, Rui; Monore, Matthew E; Pertz, Olivier C; Purvine, Samuel O; Orton, Daniel J; Jacobs, Jon M; Camp, David G; Smith, Richard D; Klemke, Richard L

    2011-05-20

    Brain development and spinal cord regeneration require neurite sprouting and growth cone navigation in response to extension and collapsing factors present in the extracellular environment. These external guidance cues control neurite growth cone extension and retraction processes through intracellular protein phosphorylation of numerous cytoskeletal, adhesion, and polarity complex signaling proteins. However, the complex kinase/substrate signaling networks that mediate neuritogenesis have not been investigated. Here, we compare the neurite phosphoproteome under growth and retraction conditions using neurite purification methodology combined with mass spectrometry. More than 4000 non-redundant phosphorylation sites from 1883 proteins have been annotated and mapped to signaling pathways that control kinase/phosphatase networks, cytoskeleton remodeling, and axon/dendrite specification. Comprehensive informatics and functional studies revealed a compartmentalized ERK activation/deactivation cytoskeletal switch that governs neurite growth and retraction, respectively. Our findings provide the first system-wide analysis of the phosphoprotein signaling networks that enable neurite growth and retraction and reveal an important molecular switch that governs neuritogenesis.

  5. Immunohistochemical profiling of caspase signaling pathways predicts clinical response to chemotherapy in primary nodal diffuse large B-cell lymphomas.

    NARCIS (Netherlands)

    Muris, J.J.; Cillessen, S.A.; Vos, W.; Houdt, I.S. van; Kummer, J.A.; Krieken, J.H.J.M. van; Jiwa, N.M.; Jansen, P.A.M.; Kluin-Nelemans, H.C.; Ossenkoppele, G.J.; Gundy, C.; Meijer, C.J.M.; Oudejans, J.J.

    2005-01-01

    We used biopsy specimens of primary nodal diffuse large B-cell lymphoma (DLBCL) to investigate whether the inhibition of caspase 8 and/or 9 apoptosis signaling pathways predicts clinical outcome. Expression levels of cellular FLICE inhibitory protein (c-Flip) and numbers of active caspase 3-positive

  6. Immunohistochemical profiling of caspase signaling pathways predicts clinical response to chemotherapy in primary nodal diffuse large B-cell lymphomas

    NARCIS (Netherlands)

    Muris, JJF; Cillessen, SAGM; Vos, W; van Houdt, IS; Kummer, JA; van Krieken, JHJM; Jiwa, NM; Jansen, PM; Kluin-Nelemans, HC; Ossenkoppele, GJ; Gundy, C; Meijer, CJLM; Oudejans, JJ

    2005-01-01

    We used biopsy specimens of primary nodal diffuse large B-cell lymphoma (DLBCL) to investigate whether the inhibition of caspase 8 and/or 9 apoplosis signaling pathways predicts clinical outcome. Expression levels of cellular FLICE inhibitory protein (c-Flip) and numbers of active caspase 3-positive

  7. Steepening of the 820 micron continuum surface-brightness profile signals dust evolution in TW Hya's disk

    CERN Document Server

    Hogerheijde, Michiel R; Pinilla, Paola; Salinas, Vachail N; Kama, Mihkel; Andrews, Sean M; Qi, Chunhua; Wilner, David J

    2015-01-01

    Grain growth in planet-forming disks is the first step toward the formation of planets. The growth of grains and their inward drift leaves a distinct imprint on the dust surface-density distribution and the resulting surface-brightness profile of the thermal continuum emission. We determine the surface-brightness profile of the continuum emission using resolved observations at millimeter wavelengths of the disk around TW Hya, and infer the signature of dust evolution on the surface density and dust opacity. Archival ALMA observations at 820 micron on baselines up to 410 kilolambda are compared to parametrized disk models to determine the surface-brightness profile. Under the assumption of a constant dust opacity, a broken radial power law best describes the dust surface density, with a slope of -0.53 +/- 0.01 from the 4.1 au radius of the (already known) inner hole to a turn-over radius of 47.1 +/- 0.2 au, steepening to -8.0 +/- 0.1 at larger radii. The emission drops below the detection limit beyond ~60 au. ...

  8. Analysis of the photometric and astrometric fidelity of high-resistivity, p- channel CCDs

    Science.gov (United States)

    Abunaemeh, Malek Amir Mahmoud

    Photometry and astrometry performed with charge coupled devices (CCDs) at the focal planes of large telescopes are indispensable tools of modern observational cosmology, astrophysics and astronomy. In the modern era of precision cosmology, variations in the sub-pixel sensitivity and spectral response of CCDs can affect the science yield of observations and must be characterized. Unfortunately, there have been very few studies to measure the sub-pixel response variations of CCDs, particularly in the context of observational cosmology. It is the aim of this thesis to perform the first measurement of the photometric and astrometric fidelity of high-resistivity, p- channel CCDs. These devices have been selected for major upcoming observational cosmology missions such as the space-based Supernova Acceleration Probe satellite (SNAP) and the ground-based Dark Energy Survey. An experimental study has been performed to make detailed measurements of the intrapixel response variations of these devices at a precision exceeding 2%, which is the level of precision required for the missions mentioned above. A 300 mm thick, 10.5 mm pixel pitch, 1.4k×1.4k format, high-resistivity, p-channel CCD operated fully depleted was illuminated by a 1.3 mm pinhole projector. The illuminated spot was moved in sub-pixel steps through various patterns to measure several properties of the device including the lateral charge diffusion, the intrapixel sensitivity variations, the effective diffusion near the edge of the device active region where electric field lines in the device may diverge, to test the photometric performance of a new technique for acquiring dithered astronomical observations coined "CCD Phase Dithering." It was determined that the intrapixel sensitivity variations were less than ˜ 0.5% in most cases. The lateral diffusion in the device was measured to be 7.41 mm in the device center, consistent with theoretical predictions. Charge spreading near the device edge resulted in an

  9. Parallax of a Mira variable R Ursae Majoris studied with astrometric VLBI

    Science.gov (United States)

    Nakagawa, Akiharu; Kurayama, Tomoharu; Matsui, Makoto; Omodaka, Toshihiro; Honma, Mareki; Shibata, Katsunori M.; Sato, Katsuhisa; Jike, Takaaki

    2016-10-01

    We have measured an annual parallax of the Mira variable R Ursae Majoris (R UMa) with the VLBI Exploration for Radio Astronomy (VERA). From the monitoring VLBI observations over a span of about two years, we detected H2O maser spots in the LSR velocity range from 37 to 42 km s-1. We derived an annual parallax of 1.97 ± 0.05 mas, and this gives a corresponding distance of 508 ± 13 pc. The VLBI maps revealed 72 maser spots distributed in an ˜110 au area around the expected stellar position. Circumstellar kinematics of the maser spots were also revealed by subtracting a systemic motion in the Hipparcos catalog from proper motions of each maser spot derived from our VLBI observations. Infrared photometry was also conducted to measure a K-band apparent magnitude, and we obtained a mean magnitude of mK = 1.19 ± 0.02 mag. Using the trigonometric distance, mK is converted to a K-band absolute magnitude of MK = -7.34 ± 0.06 mag. This result gives a much more accurate absolute magnitude for R UMa than previously provided. We solved a zero-point of the MK-log P relation for the Galactic Mira variables and obtained a relation of MK = -3.52 log P + (1.09 ± 0.14). Other long-period variables, including red supergiants, whose distances were determined with astrometric VLBI, were also compiled to explore the different sequences of the MK-log P relation.

  10. Speckle observations with PISCO in Merate: IV. Astrometric measurements of visual binaries in 2005

    Science.gov (United States)

    Scardia, M.; Prieur, J.-L.; Pansecchi, L.; Argyle, R. W.; Sala, M.; Basso, S.; Ghigo, M.; Koechlin, L.; Aristidi, E.

    2008-01-01

    We present relative astrometric measurements of visual binaries made during the second semester of 2005, with the speckle camera PISCO at the 102 cm Zeiss telescope of Brera Astronomical Observatory, in Merate. Our sample contains orbital couples as well as binaries whose motion is still uncertain. The purpose of this long term program is to improve the accuracy of the orbits and determine the masses of the components.\\ We performed 130 new observations of 120 objects, with most of the angular separations in the range 0\\farcs1-4\\arcsec, and with an average accuracy of 0\\farcs01. Most of the position angles could be determined without the usual 180° ambiguity with the application of triple-correlation techniques, and their mean error is 0\\fdg8. We have found a possible new triple system: ADS 11077. škip0.15cm The measurements of the closest binaries were made with a new data reduction procedure, based on model fitting of the background of the auto-correlations. As this procedure proved to be very efficient, we have re-processed the old observations of close binaries made with PISCO in Merate since 2004. We thus improved 20 measurements already published and obtained 7 new measurements for observations that were previously reported as ``unresolved".\\ We finally present revised orbits for ADS 684, MCA 55Aac (in the Beta 1 Cyg-Albireo multiple system) and ADS 14783 for which the previously published orbits led to large residuals with our measurements and for which the new observations made since their computation allowed a significant improvement of those old orbits. The sum of the masses that we derived for those systems are consistent with the spectral type of the stars and the dynamic parallaxes are in good agreement with the parallaxes measured by Hipparcos.

  11. Transcriptomic profiling of soybean in response to high-intensity UV-B irradiation reveals stress defense signaling

    Directory of Open Access Journals (Sweden)

    Min Young Yoon

    2016-12-01

    Full Text Available The depletion of the ozone layer in the stratosphere has led to a dramatic spike in ultraviolet B (UV-B intensity and increased UV-B light levels. The direct absorption of high-intensity UV-B induces complex abiotic stresses in plants, including excessive light exposure, heat, and dehydration. However, UV-B stress signaling mechanisms in plants including soybean (Glycine max [L.] remain poorly understood. Here, we surveyed the overall transcriptional responses of two soybean genotypes, UV-B-sensitive Cheongja 3 and UV-B-resistant Buseok, to continuous UV-B irradiation for 0 (control, 0.5, and 6 h using RNA-seq analysis. Homology analysis using UV-B-related genes from Arabidopsis thaliana revealed differentially expressed genes (DEGs likely involved in UV-B stress responses. Functional classification of the DEGs showed that the categories of immune response, stress defense signaling, and reactive oxygen species (ROS metabolism were over-represented. UV-B-resistant Buseok utilized phosphatidic acid-dependent signaling pathways (based on subsequent reactions of phospholipase C and diacylglycerol kinase rather than phospholipase D in response to UV-B exposure at high fluence rates, and genes involved in its downstream pathways, such as ABA signaling, mitogen-activated protein kinase cascades, and ROS overproduction, were upregulated in this genotype. In addition, the DEGs for TIR-NBS-LRR and heat shock proteins are positively activated. These results suggest that defense mechanisms against UV-B stress at high fluence rates are separate from the photomorphogenic responses utilized by plants to adapt to low-level UV light. Our study provides valuable information for deep understanding of UV-B stress defense mechanisms and for the development of resistant soybean genotypes that survive under high-intensity UV-B stress.

  12. Gene Expression Profile Reveals Abnormalities of Multiple Signaling Pathways in Mesenchymal Stem Cell Derived from Patients with Systemic Lupus Erythematosus

    Directory of Open Access Journals (Sweden)

    Yu Tang

    2012-01-01

    Full Text Available We aimed to compare bone-marrow-derived mesenchymal stem cells (BMMSCs between systemic lupus erythematosus (SLE and normal controls by means of cDNA microarray, immunohistochemistry, immunofluorescence, and immunoblotting. Our results showed there were a total of 1, 905 genes which were differentially expressed by BMMSCs derived from SLE patients, of which, 652 genes were upregulated and 1, 253 were downregulated. Gene ontology (GO analysis showed that the majority of these genes were related to cell cycle and protein binding. Pathway analysis exhibited that differentially regulated signal pathways involved actin cytoskeleton, focal adhesion, tight junction, and TGF-β pathway. The high protein level of BMP-5 and low expression of Id-1 indicated that there might be dysregulation in BMP/TGF-β signaling pathway. The expression of Id-1 in SLE BMMSCs was reversely correlated with serum TNF-α levels. The protein level of cyclin E decreased in the cell cycling regulation pathway. Moreover, the MAPK signaling pathway was activated in BMMSCs from SLE patients via phosphorylation of ERK1/2 and SAPK/JNK. The actin distribution pattern of BMMSCs from SLE patients was also found disordered. Our results suggested that there were distinguished differences of BMMSCs between SLE patients and normal controls.

  13. Analysis of global gene expression profile of rice in response to methylglyoxal indicates its possible role as a stress signal molecule

    Directory of Open Access Journals (Sweden)

    Charanpreet eKaur

    2015-09-01

    Full Text Available Methylglyoxal (MG is a toxic metabolite produced primarily as a byproduct of glycolysis. Being a potent glycating agent, it can readily bind macromolecules like DNA, RNA or proteins, modulating their expression and activity. In plants, despite the known inhibitory effects of MG on growth and development, still limited information is available about the molecular mechanisms and response pathways elicited upon elevation in MG levels. To gain insight into the molecular basis of MG response, we have investigated changes in global gene expression profiles in rice upon exposure to exogenous MG using GeneChip microarrays. Initially, growth of rice seedlings was monitored in response to increasing MG concentrations which could retard plant growth in a dose-dependent manner. Upon exposure to 10 mM concentration of MG, a total of 1685 probe sets were up- or down-regulated by more than 1.5-fold in shoot tissues within 16 h. These were classified into ten functional categories. The genes involved in signal transduction such as, protein kinases and transcription factors, were significantly over-represented in the perturbed transcriptome, of which several are known to be involved in abiotic and biotic stress response indicating a cross-talk between MG-responsive and stress-responsive signal transduction pathways. Through in silico studies, we could predict 7-8 bp long conserved motif as a possible MG-responsive element (MGRE in the 1 kb upstream region of genes that were more than ten-fold up- or down-regulated in the analysis. Since several perturbations were found in signaling cascades in response to MG, we hereby suggest that it plays an important role in signal transduction probably acting as a stress signal molecule.

  14. Conjugated bilirubin affects cytokine profiles in hepatitis A virus infection by modulating function of signal transducer and activator of transcription factors.

    Science.gov (United States)

    Castro-García, Flor P; Corral-Jara, Karla F; Escobedo-Melendez, Griselda; Sandoval-Hernandez, Monserrat A; Rosenstein, Yvonne; Roman, Sonia; Panduro, Arturo; Fierro, Nora A

    2014-12-01

    Hepatitis A virus (HAV) infection is the major cause of acute liver failure in paediatric patients. The clinical spectrum of infection is variable, and liver injury is determined by altered hepatic enzyme function and bilirubin concentration. We recently reported differences in cytokine profiles between distinct HAV-induced clinical courses, and bilirubin has been recognized as a potential immune-modulator. However, how bilirubin may affect cytokine profiles underlying the variability in the course of infection has not been determined. Herein, we used a transcription factor (TF) binding site identification approach to retrospectively analyse cytokine expression in HAV-infected children and to predict the entire set of TFs associated with the expression of specific cytokine profiles. The results suggested that modulation of the activity of signal transducers and activators of transcription proteins (STATs) may play a central role during HAV infection. This led us to compare the degree of STAT phosphorylation in peripheral blood lymphoid cells (PBLCs) from paediatric patients with distinct levels of conjugated bilirubin (CB). Low CB levels in sera were associated with increased STAT-1 and STAT-5 phosphorylation. A positive correlation was observed between the serum interleukin-6 (IL-6) content and CB values, whereas higher levels of CB correlated with reduced serum IL-8 values and with a reduction in the proportion of PBLCs positive for STAT-5 phosphorylation. When CB was used to stimulate patients' PBLCs in vitro, the levels of IL-6 and tumour necrosis factor-α were increased. The data showed that bilirubin plays a role in STAT function and affects cytokine profile expression during HAV infection.

  15. Transcriptomic profiling of linolenic acid-responsive genes in ROS signalling from RNA-seq data in Arabidopsis

    Directory of Open Access Journals (Sweden)

    Capilla eMata-Pérez

    2015-03-01

    Full Text Available Linolenic acid (Ln released from chloroplast membrane galactolipids is a precursor of the phytohormone jasmonic acid (JA. The involvement of this hormone in different plant biological processes, such as responses to biotic stress conditions, has been extensively studied. However, the role of Ln in the regulation of gene expression during abiotic stress situations mediated by cellular redox changes and/or by oxidative stress processes remains poorly understood. An RNA-seq approach has increased our knowledge of the interplay among Ln, oxidative stress and ROS signalling that mediates abiotic stress conditions. Transcriptome analysis with the aid of RNA-seq in the absence of oxidative stress revealed that the incubation of Arabidopsis thaliana cell suspension cultures (ACSC with Ln resulted in the modulation of 7525 genes, of which 3034 genes had a 2 fold-change, being 533 up- and 2501 down-regulated genes, respectively. Thus, RNA-seq data analysis showed that an important set of these genes were associated with the jasmonic acid biosynthetic pathway including lypoxygenases (LOXs and Allene oxide cyclases (AOCs. In addition, several transcription factor families involved in the response to biotic stress conditions (pathogen attacks or herbivore feeding, such as WRKY, JAZ, MYC and LRR were also modified in response to Ln. However, this study also shows that Ln has the capacity to modulate the expression of genes involved in the response to abiotic stress conditions, particularly those mediated by ROS signalling. In this regard, we were able to identify new targets such as galactinol synthase 1 (GOLS1, methionine sulfoxide reductase (MSR and alkenal reductase in ACSC. It is therefore possible to suggest that, in the absence of any oxidative stress, Ln is capable of modulating new sets of genes involved in the signalling mechanism mediated by additional abiotic stresses (salinity, UV and high light intensity and especially in stresses mediated by ROS.

  16. Gene Expression Profiling and Molecular Signaling of Dental Pulp Cells in Response to Tricalcium Silicate Cements: A Systematic Review.

    Science.gov (United States)

    Rathinam, Elanagai; Rajasekharan, Sivaprakash; Chitturi, Ravi Teja; Martens, Luc; De Coster, Peter

    2015-11-01

    Signaling molecules and responding dental pulp stem cells are the 2 main control keys of dentin regeneration/dentinogenesis. The aim of this study was to present a systematic review investigating the gene expression of various dental pulp cells in response to different variants of tricalcium silicate cements. A systematic search of the literature was performed by 2 independent reviewers followed by article selection and data extraction. Studies analyzing all sorts of dental pulp cells (DPCs) and any variant of tricalcium silicate cement either as the experimental or as the control group were included. A total of 39 articles were included in the review. Among the included studies, ProRoot MTA (Dentsply, Tulsa Dental, OK) was the most commonly used tricalcium silicate cement variant. The extracellular signal regulated kinase/mitogen-activated protein kinase pathway was the most commonly activated pathway to be identified, and similarly, dentin sialophosphoprotein osteocalcin dentin matrix acidic phosphoprotein 1, alkaline phosphatase, bone sialoprotein, osteopontin, type I collagen, and Runx2 were the most commonly expressed genes in that order of frequency. Biodentine (Septodont Ltd, Saint Maur des Faussés, France), Bioaggregate (Innovative Bioceramix, Vancouver, BC, Canada), and mineral trioxide aggregate stimulate the osteogenic/odontogenic capacity of DPCs by proliferation, angiogenesis, and biomineralization through the activation of the extracellular signal regulated kinase ½, nuclear factor E2 related factor 2, p38, c-Jun N-terminal kinase mitogen-activated protein kinase, p42/p44 mitogen-activated protein kinase, nuclear factor kappa B, and fibroblast growth factor receptor pathways. When DPCs are placed into direct contact with tricalcium silicate cements, they show higher levels of gene activation, which in turn could translate into more effective pulpal repair and faster and more predictable formation of reparative dentin. Copyright © 2015 American

  17. Circadian profiles in the embryonic chick heart: L-type voltage-gated calcium channels and signaling pathways.

    Science.gov (United States)

    Ko, Michael L; Shi, Liheng; Grushin, Kirill; Nigussie, Fikru; Ko, Gladys Y-P

    2010-10-01

    Circadian clocks exist in the heart tissue and modulate multiple physiological events, from cardiac metabolism to contractile function and expression of circadian oscillator and metabolic-related genes. Ample evidence has demonstrated that there are endogenous circadian oscillators in adult mammalian cardiomyocytes. However, mammalian embryos cannot be entrained independently to light-dark (LD) cycles in vivo without any maternal influence, but circadian genes are well expressed and able to oscillate in embryonic stages. The authors took advantage of using chick embryos that are independent of maternal influences to investigate whether embryonic hearts could be entrained under LD cycles in ovo. The authors found circadian regulation of L-type voltage-gated calcium channels (L-VGCCs), the ion channels responsible for the production of cardiac muscle contraction in embryonic chick hearts. The mRNA levels and protein expression of VGCCα1C and VGCCα1D are under circadian control, and the average L-VGCC current density is significantly larger when cardiomyocytes are recorded during the night than day. The phosphorylation states of several kinases involved in insulin signaling and cardiac metabolism, including extracellular signal-regulated kinase (Erk), stress-activated protein kinase (p38), protein kinase B (Akt), and glycogen synthase kinase-3β (GSK-3β), are also under circadian control. Both Erk and p38 have been implicated in regulating cardiac contractility and in the development of various pathological states, such as cardiac hypertrophy and heart failure. Even though both Erk and phosphoinositide 3-kinase (PI3K)-Akt signaling pathways participate in complex cellular processes regarding physiological or pathological states of cardiomyocytes, the circadian oscillators in the heart regulate these pathways independently, and both pathways contribute to the circadian regulation of L-VGCCs.

  18. Astrometric Constraints on the Masses of Long-period Gas Giant Planets in the TRAPPIST-1 Planetary System

    Science.gov (United States)

    Boss, Alan P.; Weinberger, Alycia J.; Keiser, Sandra A.; Astraatmadja, Tri L.; Anglada-Escude, Guillem; Thompson, Ian B.

    2017-09-01

    Transit photometry of the M8V dwarf star TRAPPIST-1 (2MASS J23062928-0502285) has revealed the presence of at least seven planets with masses and radii similar to that of Earth, orbiting at distances that might allow liquid water to be present on their surfaces. We have been following TRAPPIST-1 since 2011 with the CAPSCam astrometric camera on the 2.5 m du Pont telescope at the Las Campanas Observatory in Chile. In 2016, we noted that TRAPPIST-1 lies slightly farther away than previously thought, at 12.49 pc, rather than 12.1 pc. Here, we examine 15 epochs of CAPSCam observations of TRAPPIST-1, spanning the five years from 2011 to 2016, and obtain a revised trigonometric distance of 12.56 ± 0.12 pc. The astrometric data analysis pipeline shows no evidence for a long-period astrometric wobble of TRAPPIST-1. After proper motion and parallax are removed, residuals at the level of ±1.3 mas remain. The amplitude of these residuals constrains the masses of any long-period gas giant planets in the TRAPPIST-1 system: no planet more massive than ∼4.6 M Jup orbits with a 1 year period, and no planet more massive than ∼1.6 M Jup orbits with a 5 year period. Further refinement of the CAPSCam data analysis pipeline, combined with continued CAPSCam observations, should either detect any long-period planets, or put an even tighter constraint on these mass upper limits.

  19. Astrometric calibration of the Xuyi Schmidt Telescope Photometric Survey of the Galactic Anti-center (XSTPS-GAC)

    Science.gov (United States)

    Zhang, Hui-Hua; Liu, Xiao-Wei; Yuan, Hai-Bo; Zhao, Hai-Bin; Yao, Jin-Sheng; Zhang, Hua-Wei; Xiang, Mao-Sheng; Huang, Yang

    2014-04-01

    We present astrometric calibration of the Xuyi Schmidt Telescope Photometric Survey of the Galactic Anti-center (XSTPS-GAC). XSTPS-GAC is the photometric part of the Digital Sky Survey of the Galactic Anti-center (DSS-GAC), which is a photometric and spectroscopic sky survey, in combination with LAMOST. In order to select an astrometric reference catalog, we made comparisons between the four widely used astrometric catalogs, GSC2.3, USNO-B1.0, UCAC3 and PPMXL. PPMXL shows relatively small systematic errors in positions and more homogeneous proper motion distributions toward the Galactic Anti-center (GAC), and was selected as the reference catalog. Based on the high quality and bright reference stars that were picked out from PPMXL, we performed a 4th-order polynomial fitting in image units, to construct the transformation relation between coordinates used by XSTPS-GAC and standard coordinates, and to simultaneously correct the image distortions in the CCD. Then we applied the derived relation to all sources to obtain their mean celestial coordinates based on the International Celestial Reference System. For bright point sources with r errors being less than 10 mas. But for the faint sources at the brightness limit of the survey, which was r ~ 19.0 mag, the accuracy can still reach 200 mas. After combining all observations, the final weighted average coordinates could reach an accuracy of less than 70 mas for bright stars. For faint stars, the rms residuals of weighted coordinates decrease to ~ 110 mas. The final combined XSTPS-GAC coordinates show a good consistency with the Sloan Digital Sky Survey.

  20. Distinct expression profiles of TGF-β1 signaling mediators in pathogenic SIVmac and non-pathogenic SIVagm infections

    Directory of Open Access Journals (Sweden)

    Butor Cécile

    2006-06-01

    Full Text Available Abstract Background The generalized T-cell activation characterizing HIV-1 and SIVmac infections in humans and macaques (MACs is not found in the non-pathogenic SIVagm infection in African green monkeys (AGMs. We have previously shown that TGF-β1, Foxp3 and IL-10 are induced very early after SIVagm infection. In SIVmac-infected MACs, plasma TGF-β1 induction persists during primary infection 1. We raised the hypothesis that MACs are unable to respond to TGF-β1 and thus cannot resorb virus-driven inflammation. We therefore compared the very early expression dynamics of pro- and anti-inflammatory markers as well as of factors involved in the TGF-β1 signaling pathway in SIV-infected AGMs and MACs. Methods Levels of transcripts encoding for pro- and anti-inflammatory markers (tnf-α, ifn-γ, il-10, t-bet, gata-3 as well as for TGF-β1 signaling mediators (smad3, smad4, smad7 were followed by real time PCR in a prospective study enrolling 6 AGMs and 6 MACs. Results During primary SIVmac infection, up-regulations of tnf-α, ifn-γ and t-bet responses (days 1–16 p.i. were stronger whereas il-10 response was delayed (4th week p.i. compared to SIVagm infection. Up-regulation of smad7 (days 3–8 p.i., a cellular mediator inhibiting the TGF-β1 signaling cascade, characterized SIV-infected MACs. In AGMs, we found increases of gata-3 but not t-bet, a longer lasting up-regulation of smad4 (days 1–21 p.i, a mediator enhancing TGF-β1 signaling, and no smad7 up-regulations. Conclusion Our data suggest that the inability to resorb virus-driven inflammation and activation during the pathogenic HIV-1/SIVmac infections is associated with an unresponsiveness to TGF-β1.

  1. Chemical probes for competitive profiling of the quorum sensing signal synthase PqsD of Pseudomonas aeruginosa

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    Michaela Prothiwa

    2016-12-01

    Full Text Available The human pathogen Pseudomonas aeruginosa uses the pqs quorum sensing system to coordinate the production of its broad spectrum of virulence factors to facilitate colonization and infection of its host. Hereby, the enzyme PqsD is a virulence related quorum sensing signal synthase that catalyzes the central step in the biosynthesis of the Pseudomonas quinolone signals HHQ and PQS. We developed a library of cysteine reactive chemical probes with an alkyne handle for fluorescence tagging and report the selective and highly sensitive in vitro labelling of the active site cysteine of this important enzyme. Interestingly, only one type of probe, with a reactive α-chloroacetamide was capable of covalently reacting with the active site. We demonstrated the potential of our probes in a competitive labelling platform where we screened a library of synthetic HHQ and PQS analogues with heteroatom replacements and found several inhibitors of probe binding that may represent promising scaffolds for the development of customized PqsD inhibitors as well as a chemical toolbox to investigate the activity and active site specificity of the enzyme.

  2. Isolation and molecular characterization of biofouling bacteria and profiling of quorum sensing signal molecules from membrane bioreactor activated sludge.

    Science.gov (United States)

    Lade, Harshad; Paul, Diby; Kweon, Ji Hyang

    2014-02-04

    The formation of biofilm in a membrane bioreactor depends on the production of various signaling molecules like N-acyl homoserine lactones (AHLs). In the present study, a total of 200 bacterial strains were isolated from membrane bioreactor activated sludge and screened for AHLs production using two biosensor systems, Chromobacterium violaceum CV026 and Agrobacterium tumefaciens A136. A correlation between AHLs production and biofilm formation has been made among screened AHLs producing strains. The 16S rRNA gene sequence analysis revealed the dominance of Aeromonas and Enterobacter sp. in AHLs production; however few a species of Serratia, Leclercia, Pseudomonas, Klebsiella, Raoultella and Citrobacter were also identified. The chromatographic characterization of sludge extract showed the presence of a broad range of quorum sensing signal molecules. Further identification of sludge AHLs by thin layer chromatography bioassay and high performance liquid chromatography confirms the presence of C4-HSL, C6-HSL, C8-HSL, 3-oxo-C8-HSL, C10-HSL, C12-HSL, 3-oxo-C12-HSL and C14-HSL. The occurrence of AHLs in sludge extract and dominance of Aeromonas and Enterobacter sp. in activated sludge suggests the key role of these bacterial strains in AHLs production and thereby membrane fouling.

  3. Isolation and Molecular Characterization of Biofouling Bacteria and Profiling of Quorum Sensing Signal Molecules from Membrane Bioreactor Activated Sludge

    Directory of Open Access Journals (Sweden)

    Harshad Lade

    2014-02-01

    Full Text Available The formation of biofilm in a membrane bioreactor depends on the production of various signaling molecules like N-acyl homoserine lactones (AHLs. In the present study, a total of 200 bacterial strains were isolated from membrane bioreactor activated sludge and screened for AHLs production using two biosensor systems, Chromobacterium violaceum CV026 and Agrobacterium tumefaciens A136. A correlation between AHLs production and biofilm formation has been made among screened AHLs producing strains. The 16S rRNA gene sequence analysis revealed the dominance of Aeromonas and Enterobacter sp. in AHLs production; however few a species of Serratia, Leclercia, Pseudomonas, Klebsiella, Raoultella and Citrobacter were also identified. The chromatographic characterization of sludge extract showed the presence of a broad range of quorum sensing signal molecules. Further identification of sludge AHLs by thin layer chromatography bioassay and high performance liquid chromatography confirms the presence of C4-HSL, C6-HSL, C8-HSL, 3-oxo-C8-HSL, C10-HSL, C12-HSL, 3-oxo-C12-HSL and C14-HSL. The occurrence of AHLs in sludge extract and dominance of Aeromonas and Enterobacter sp. in activated sludge suggests the key role of these bacterial strains in AHLs production and thereby membrane fouling.

  4. A deep investigation into the adipogenesis mechanism: Profile of microRNAs regulating adipogenesis by modulating the canonical Wnt/β-catenin signaling pathway

    Directory of Open Access Journals (Sweden)

    Li Jing

    2010-05-01

    Full Text Available Abstract Background MicroRNAs (miRNAs are a large class of tiny non-coding RNAs (~22-24 nt that regulate diverse biological processes at the posttranscriptional level by controlling mRNA stability or translation. As a molecular switch, the canonical Wnt/β-catenin signaling pathway should be suppressed during the adipogenesis; However, activation of this pathway leads to the inhibition of lipid depots formation. The aim of our studies was to identify miRNAs that might be involved in adipogenesis by modulating WNT signaling pathway. Here we established two types of cell model, activation and repression of WNT signaling, and investigated the expression profile of microRNAs using microarray assay. Results The high throughput microarray data revealed 18 miRNAs that might promote adipogenesis by repressing WNT signaling: miR-210, miR-148a, miR-194, miR-322 etc. Meanwhile, we also identified 29 miRNAs that might have negative effect on adipogenesis by activating WNT signaling: miR-344, miR-27 and miR-181 etc. The targets of these miRNAs were also analysed by bioinformatics. To validate the predicted targets and the potential functions of these identified miRNAs, the mimics of miR-210 were transfected into 3T3-L1 cells and enlarged cells with distinct lipid droplets were observed; Meanwhile, transfection with the inhibitor of miR-210 could markedly decrease differentiation-specific factors at the transcription level, which suggested the specific role of miR-210 in promoting adipogenesis. Tcf7l2, the predicted target of miR-210, is a transcription factor triggering the downstream responsive genes of WNT signaling, was blocked at transcription level. Furthermore, the activity of luciferase reporter bearing Tcf7l2 mRNA 3' UTR was decreased after co-transfection with miR-210 in HEK-293FT cells. Last but not least, the protein expression level of β-catenin was increased in the lithium (LiCl treated 3T3-L1 cells after transfection with miR-210. These

  5. Verification of the Astrometric Performance of the Korean VLBI Network, using comparative SFPR studies with the VLBA at 14/7 mm

    CERN Document Server

    Rioja, Mar\\'\\ia J; Jung, TaeHyun; Sohn, Bong Won; Byun, Do-Young; Agudo, Iván; Cho, Se-Hyung; Lee, Sang-Sung; Kim, Jongsoo; Kim, Kee-Tae; Oh, Chung Sik; Han, Seog-Tae; Je, Do-Heung; Chung, Moon-Hee; Wi, Seog-Oh; Kang, Jiman; Lee, Jung-Won; Chung, Hyunsoo; Kim, Hyo Ryoung; Kim, Hyun-Goo; Lee, Chang-Hoon; Roh, Duk-Gyoo; Oh, Se-Jin; Yeom, Jae-Hwan; Song, Min-Gyu; Kang, Yong-Woo

    2014-01-01

    The Korean VLBI Network (KVN) is a new mm-VLBI dedicated array with capability for simultaneous observations at multiple frequencies, up to 129 GHz. The innovative multi-channel receivers present significant benefits for astrometric measurements in the frequency domain. The aim of this work is to verify the astrometric performance of the KVN using a comparative study with the VLBA, a well established instrument. For that purpose, we carried out nearly contemporaneous observations with the KVN and the VLBA, at 14/7 mm, in April 2013. The KVN observations consisted of simultaneous dual frequency observations, while the VLBA used fast frequency switching observations. We used the Source Frequency Phase Referencing technique for the observational and analysis strategy. We find that having simultaneous observations results in a superior performance for compensation of all atmospheric terms in the observables, in addition to offering other significant benefits for astrometric analysis. We have compared the KVN astr...

  6. Update on Astrometric Follow-Up at Apache Point Observatory by Adler Planetarium

    Science.gov (United States)

    Nault, Kristie A.; Brucker, Melissa; Hammergren, Mark

    2016-10-01

    We began our NEO astrometric follow-up and characterization program in 2014 Q4 using about 500 hours of observing time per year with the Astrophysical Research Consortium (ARC) 3.5m telescope at Apache Point Observatory (APO). Our observing is split into 2 hour blocks approximately every other night for astrometry (this poster) and several half-nights per month for spectroscopy (see poster by M. Hammergren et al.) and light curve studies.For astrometry, we use the ARC Telescope Imaging Camera (ARCTIC) with an SDSS r filter, in 2 hour observing blocks centered around midnight. ARCTIC has a magnitude limit of V~23 in 60s, and we target 20 NEOs per session. ARCTIC has a FOV 1.57 times larger and a readout time half as long as the previous imager, SPIcam, which we used from 2014 Q4 through 2015 Q3. Targets are selected primarily from the Minor Planet Center's (MPC) NEO Confirmation Page (NEOCP), and NEA Observation Planning Aid; we also refer to JPL's What's Observable page, the Spaceguard Priority List and Faint NEOs List, and requests from other observers. To quickly adapt to changing weather and seeing conditions, we create faint, midrange, and bright target lists. Detected NEOs are measured with Astrometrica and internal software, and the astrometry is reported to the MPC.As of June 19, 2016, we have targeted 2264 NEOs, 1955 with provisional designations, 1582 of which were detected. We began observing NEOCP asteroids on January 30, 2016, and have targeted 309, 207 of which were detected. In addition, we serendipitously observed 281 moving objects, 201 of which were identified as previously known objects.This work is based on observations obtained with the Apache Point Observatory 3.5m telescope, which is owned and operated by the Astrophysical Research Consortium. We gratefully acknowledge support from NASA NEOO award NNX14AL17G and thank the University of Chicago Department of Astronomy and Astrophysics for observing time in 2014.

  7. Stellar, Remnant, Planetary, and Dark-Object Masses from Astrometric Microlensing

    Science.gov (United States)

    Gould, Andrew P.; Bennett, David P.; Boden, Andrew; Depoy, Darren L.; Gaudi, Scott B.; Griest, Kim; Han, Cheongho; Paczynski, Bohdan; Reid, I. Neill

    2004-01-01

    The primary goal of our project is to make a complete census of the stellar population of the Galaxy. We are broadening the term stellar here to include both ordinary stars and dark stars. Ordinary stars, burning their nuclear fuel and shining, can perhaps best be studied with traditional astronomical techniques, but dark stars, by which we include old brown dwarfs, black holes, old white dwarfs, neutron stars, and perhaps exotic objects such as mirror matter stars or primordial black holes, can only be studied by their gravitational effects. Traditionally, these objects have been probed in binaries, and thus selected in a way that may or may not be representative of their respective field populations. The only way to examine the field population of these stars is through microlensing, the deflection of light from a visible star in the background by an object (dark or not) in the foreground. When lensed, there are two images of the background star. Although these images cannot be resolved when the lens has a stellar mass, the lensing effect can be detected in two ways: photometrically, i.e. by measuring the magnification of the source by the lens, and astrometrically, i.e. by measuring the shift in the centroid of the two images. Photometric microlensing experiments have detected hundreds of microlensing events over the past decade. Despite its successes, photometric microlensing has so far been somewhat frustrating because these events are difficult to interpret. Almost nothing is known about the masses of individual lenses and very little is known about the statistical properties of the lenses treated as a whole, such as their average mass. Although probably over 100 of the lenses are in fact dark objects, we can't determine which they are, let alone investigate finer details such as what their masses are, and where they are in the Galaxy. With SIM, we will break the microlensing degeneracy, and allow detailed interpretation of individual microlensing events. We

  8. Expression profiles of genes involved in jasmonic acid biosynthesis and signaling during growth and development of carrot.

    Science.gov (United States)

    Wang, Guanglong; Huang, Wei; Li, Mengyao; Xu, Zhisheng; Wang, Feng; Xiong, Aisheng

    2016-09-01

    Jasmonates (JAs) are recognized as essential regulators in response to environmental stimuli and plant development. Carrot is an Apiaceae vegetable with great value and undergoes significant size changes over the course of plant growth. However, JA accumulation and its potential roles in carrot growth remain unclear. Here, methyl JA (MeJA) levels and expression profiles of JA-related genes were analyzed in carrot roots and leaves at five developmental stages. MeJA levels in the roots and leaves were the highest at the first stage and decreased as carrot growth proceeded. Transcript levels of several JA-related genes (Dc13-LOX1, Dc13-LOX2, DcAOS, DcAOC, DcOPR2, DcOPR3, DcOPCL1, DcJAR1, DcJMT, DcCOI1, DcJAZ1, DcJAZ2, DcMYC2, DcCHIB/PR3, DcLEC, and DcVSP2) were not well correlated with MeJA accumulation during carrot root and leaf development. In addition, some JA-related genes (DcJAR1, DcJMT, DcCOI1, DcMYC2, and DcVSP2) showed differential expression between roots and leaves. These results suggest that JAs may regulate carrot plant growth in stage-dependent and organ-specific manners. Our work provides novel insights into JA accumulation and its potential roles during carrot growth and development.

  9. Influence of the Laser Spot Size, Focal Beam Profile, and Tissue Type on the Lipid Signals Obtained by MALDI-MS Imaging in Oversampling Mode

    Science.gov (United States)

    Wiegelmann, Marcel; Dreisewerd, Klaus; Soltwisch, Jens

    2016-12-01

    To improve the lateral resolution in matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) beyond the dimensions of the focal laser spot oversampling techniques are employed. However, few data are available on the effect of the laser spot size and its focal beam profile on the ion signals recorded in oversampling mode. To investigate these dependencies, we produced 2 times six spots with dimensions between 30 and 200 μm. By optional use of a fundamental beam shaper, square flat-top and Gaussian beam profiles were compared. MALDI-MSI data were collected using a fixed pixel size of 20 μm and both pixel-by-pixel and continuous raster oversampling modes on a QSTAR mass spectrometer. Coronal mouse brain sections coated with 2,5-dihydroxybenzoic acid matrix were used as primary test systems. Sizably higher phospholipid ion signals were produced with laser spots exceeding a dimension of 100 μm, although the same amount of material was essentially ablated from the 20 μm-wide oversampling pixel at all spot size settings. Only on white matter areas of the brain these effects were less apparent to absent. Scanning electron microscopy images showed that these findings can presumably be attributed to different matrix morphologies depending on tissue type. We propose that a transition in the material ejection mechanisms from a molecular desorption at large to ablation at smaller spot sizes and a concomitant reduction in ion yields may be responsible for the observed spot size effects. The combined results indicate a complex interplay between tissue type, matrix crystallization, and laser-derived desorption/ablation and finally analyte ionization.

  10. A signal amplification assay for HSV type 1 viral DNA detection using nanoparticles and direct acoustic profiling

    Directory of Open Access Journals (Sweden)

    Hammond Richard

    2010-02-01

    Full Text Available Abstract Background Nucleic acid based recognition of viral sequences can be used together with label-free biosensors to provide rapid, accurate confirmation of viral infection. To enhance detection sensitivity, gold nanoparticles can be employed with mass-sensitive acoustic biosensors (such as a quartz crystal microbalance by either hybridising nanoparticle-oligonucleotide conjugates to complimentary surface-immobilised ssDNA probes on the sensor, or by using biotin-tagged target oligonucleotides bound to avidin-modified nanoparticles on the sensor. We have evaluated and refined these signal amplification assays for the detection from specific DNA sequences of Herpes Simplex Virus (HSV type 1 and defined detection limits with a 16.5 MHz fundamental frequency thickness shear mode acoustic biosensor. Results In the study the performance of semi-homogeneous and homogeneous assay formats (suited to rapid, single step tests were evaluated utilising different diameter gold nanoparticles at varying DNA concentrations. Mathematical models were built to understand the effects of mass transport in the flow cell, the binding kinetics of targets to nanoparticles in solution, the packing geometries of targets on the nanoparticle, the packing of nanoparticles on the sensor surface and the effect of surface shear stiffness on the response of the acoustic sensor. This lead to the selection of optimised 15 nm nanoparticles that could be used with a 6 minute total assay time to achieve a limit of detection sensitivity of 5.2 × 10-12 M. Larger diameter nanoparticles gave poorer limits of detection than smaller particles. The limit of detection was three orders of magnitude lower than that observed using a hybridisation assay without nanoparticle signal amplification. Conclusions An analytical model was developed to determine optimal nanoparticle diameter, concentration and probe density, which allowed efficient and rapid optimisation of assay parameters

  11. Quasars can be used to verify the parallax zero-point of the Tycho-Gaia Astrometric Solution

    CERN Document Server

    Michalik, Daniel

    2016-01-01

    Context. The Gaia project will determine positions, proper motions, and parallaxes for more than one billion stars in our Galaxy. It is known that Gaia's two telescopes are affected by a small but significant variation of the basic angle between them. Unless this variation is taken into account during data processing, e.g. using on-board metrology, it causes systematic errors in the astrometric parameters, in particular a shift of the parallax zero-point. Previously, we suggested an early reduction of Gaia data for the subset of Tycho-2 stars (Tycho-Gaia Astrometric Solution; TGAS). Aims. We aim to investigate whether quasars can be used to independently verify the parallax zero-point already in early data reductions. This is not trivially possible as the observation interval is too short to disentangle parallax and proper motion for the quasar subset. Methods. We repeat TGAS simulations but additionally include simulated Gaia observations of quasars from ground-based surveys. All observations are simulated w...

  12. Interannaul variations of the vertical and their possible influence on the star catalogs derived from ground-based astrometric observations

    Science.gov (United States)

    Li, Z. X.

    The efforts at Shanghai Observatory since 1991, in response to the Resolution of IAU Comm.19: "Applications of optical astrometry time and latitude programs", is described in the paper, especially the studies concerned with the interannual variations of the vertical and their influence on the astronomical studies. It is clear now that there is a component of the order 0.01 - 0.02" on an interannual time scale in latitude residuals which is correlated with geophysical phenomena on the Earth. A recent study has confirmed that the component discovered is actually the variation of the vertical, related to ground-based observation in astronomy. So, it should be emphasized now that the variation of the vertical is significant enough to be considered in astronomy from now on. Its influence on the past studies, including the star catalogs already published and the ERP before 1980 when optical astrometry observations were still used, should be studied in the future. In comparing the HIPPARCOS catalog with those derived by the past observations, we should keep in mind the existence of this error in an astrometric observation and its influence on the star catalogs and other results derived from ground-based astrometric observations.

  13. Rotation periods and astrometric motions of the Luhman 16AB brown dwarfs by high-resolution lucky-imaging monitoring

    CERN Document Server

    Mancini, L; Littlefair, S P; Southworth, J; Bozza, V; Damasso, M; Dominik, M; Hundertmark, M; Jorgensen, U G; Juncher, D; Popovas, A; Rabus, M; Rahvar, S; Schmidt, R W; Skottfelt, J; Snodgrass, C; Sozzetti, A; Alsubai, K; Bramich, D M; Novati, S Calchi; Ciceri, S; D'Ago, G; Jaimes, R Figuera; Galianni, P; Gu, S -H; Harpsoe, K; Haugbolle, T; Henning, Th; Hinse, T C; Kains, N; Korhonen, H; Scarpetta, G; Starkey, D; Surdej, J; Wang, X -B; Wertz, O

    2015-01-01

    Context. Photometric monitoring of the variability of brown dwarfs can provide useful information about the structure of clouds in their cold atmospheres. The brown-dwarf binary system Luhman 16AB is an interesting target for such a study, as its components stand at the L/T transition and show high levels of variability. Luhman 16AB is also the third closest system to the Solar system, allowing precise astrometric investigations with ground-based facilities. Aims. The aim of the work is to estimate the rotation period and study the astrometric motion of both components. Methods. We have monitored Luhman 16AB over a period of two years with the lucky-imaging camera mounted on the Danish 1.54m telescope at La Silla, through a special i+z long-pass filter, which allowed us to clearly resolve the two brown dwarfs into single objects. An intense monitoring of the target was also performed over 16 nights, in which we observed a peak-to-peak variability of 0.20 \\pm 0.02 mag and 0.34 \\pm 0.02 mag for Luhman 16A and 1...

  14. Distortion of the pixel grid in HST WFC3/UVIS and ACS/WFC CCD detectors and its astrometric correction

    Science.gov (United States)

    Kozhurina-Platais, Vera; Mackenty, John; Golimovski, David; Sirianni, Marco; Borncamp, David; Anderson, Jay; Grogin, Norman

    2016-07-01

    The geometric distortion of the CCD detectors used in the Hubble Space TelescopeWide Field Camera 3 (WFC3) and Advanced Camera for Surveys (ACS) instruments is characterized by both large and fine-scale distortions. The large-scale distortion, due to the complexity of the HST optical assembly, can be modeled by a high-order polynomial. The majority of fine-distortion is inherent to the CCD detectors themselves, which manifests itself as fine-scale, correlated systematic offsets in the residuals from the best-fit polynomial solution. Such systematic offsets across the CCD chip introduce astrometric errors at the level of about 0.1 pix (up to 1.5 μm within the 15 μm pixels). These fine-scale and low-amplitude distortions apparently arise from the spatial irregularities in the pixel grid. For the WFC3/UVIS CCD chips, there is a clear pattern of periodic skew in the lithographic-mask stencil imprinted onto the detector. Similar irregularities in the pixel grid of ACS/WFC CCD chips are even more pronounced by the narrow (68×2048 pixel) lithographic-mask stencil. To remove these distortions, a 2-D correction in the form of a look-up table has been developed using HST images of very dense stellar fields. The post-correction of fine-scale astrometric errors can be removed down to the level of 0.01 pix (0.15 μm) or better.

  15. Astrometric Confirmation and Preliminary Orbital Parameters of the Young Exoplanet 51 Eridani b with the Gemini Planet Imager

    CERN Document Server

    De Rosa, Robert J; Blunt, Sarah C; Graham, James R; Konopacky, Quinn M; Marois, Christian; Pueyo, Laurent; Rameau, Julien; Wang, Jason J; Bailey, Vanessa; Chontos, Ashley; Fabrycky, Daniel C; Follette, Katherine B; Macintosh, Bruce; Marchis, Franck; Ammons, S Mark; Arriaga, Pauline; Chilcote, Jeffrey K; Doyon, René; Duchêne, Gaspard; Esposito, Thomas M; Fitzgerald, Michael P; Gerard, Benjamin; Goodsell, Stephen J; Greenbaum, Alexandra Z; Hibon, Pascale; Ingraham, Patrick; Johnson-Groh, Mara; Kalas, Paul G; Lafrenière, David; Maire, Jerome; Metchev, Stanimir; Millar-Blanchaer, Maxwell A; Morzinski, Katie M; Oppenheimer, Rebecca; Patel, Rahul I; Patience, Jennifer L; Perrin, Marshall D; Rajan, Abhijith; Rantakyrö, Fredrik T; Ruffio, Jean-Baptiste; Schneider, Adam C; Sivaramakrishnan, Anand; Song, Inseok; Tran, Debby; Ward-Duong, Kimberly; Wolff, Schuyler G

    2015-01-01

    We present new GPI observations of the young exoplanet 51 Eridani b which provide further evidence that the companion is physically associated with 51 Eridani. Combining this new astrometric measurement with those reported in the literature, we significantly reduce the posterior probability that 51 Eridani b is an unbound foreground or background T-dwarf in a chance alignment with 51 Eridani to $2\\times10^{-7}$, an order of magnitude lower than previously reported. If 51 Eridani b is indeed a bound object, then we have detected orbital motion of the planet between the discovery epoch and the latest epoch. By implementing a computationally efficient Monte Carlo technique, preliminary constraints are placed on the orbital parameters of the system. The current set of astrometric measurements suggest an orbital semi-major axis of $14^{+7}_{-3}$ AU, corresponding to a period of $41^{+35}_{-12}$ yr (assuming a mass of $1.75$ M$_{\\odot}$ for the central star), and an inclination of $138^{+15}_{-13}$ deg. The remaini...

  16. Genome-wide expression profiling in the Drosophila eye reveals unexpected repression of Notch signaling by the JAK/STAT pathway

    Science.gov (United States)

    Flaherty, Maria Sol; Zavadil, Jiri; Ekas, Laura A.; Bach, Erika A.

    2010-01-01

    Although the JAK/STAT pathway regulates numerous processes in vertebrates and invertebrates through modulating transcription, its functionally-relevant transcriptional targets remain largely unknown. With one jak and one stat (stat92E), Drosophila provides a powerful system for finding new JAK/STAT target genes. Genome-wide expression profiling on eye discs in which Stat92E is hyperactivated, revealed 584 differentially-regulated genes, including known targets domeless, socs36E and wingless. Other differentially-regulated genes (chinmo, lama, Mo25, Imp-L2, Serrate, Delta) were validated and may represent new Stat92E targets. Genetic experiments revealed that Stat92E cell-autonomously represses Serrate, which encodes a Notch ligand. Loss of Stat92E led to de-repression of Serrate in the dorsal eye, resulting in ectopic Notch signaling and aberrant eye growth there. Thus, our micro-array documents a new Stat92E target gene and a previously-unidentified inhibitory action of Stat92E on Notch signaling. These data suggest that this study will be a useful resource for the identification of additional Stat92E targets. PMID:19504457

  17. A functional glycoprotein competitive recognition and signal amplification strategy for carbohydrate-protein interaction profiling and cell surface carbohydrate expression evaluation.

    Science.gov (United States)

    Wang, Yangzhong; Chen, Zhuhai; Liu, Yang; Li, Jinghong

    2013-08-21

    A simple and sensitive carbohydrate biosensor has been suggested as a potential tool for accurate analysis of cell surface carbohydrate expression as well as carbohydrate-based therapeutics for a variety of diseases and infections. In this work, a sensitive biosensor for carbohydrate-lectin profiling and in situ cell surface carbohydrate expression was designed by taking advantage of a functional glycoprotein of glucose oxidase acting as both a multivalent recognition unit and a signal amplification probe. Combining the gold nanoparticle catalyzed luminol electrogenerated chemiluminescence and nanocarrier for active biomolecules, the number of cell surface carbohydrate groups could be conveniently read out. The apparent dissociation constant between GOx@Au probes and Con A was detected to be 1.64 nM and was approximately 5 orders of magnitude smaller than that of mannose and Con A, which would arise from the multivalent effect between the probe and Con A. Both glycoproteins and gold nanoparticles contribute to the high affinity between carbohydrates and lectin. The as-proposed biosensor exhibits excellent analytical performance towards the cytosensing of K562 cells with a detection limit of 18 cells, and the mannose moieties on a single K562 cell were determined to be 1.8 × 10(10). The biosensor can also act as a useful tool for antibacterial drug screening and mechanism investigation. This strategy integrates the excellent biocompatibility and multivalent recognition of glycoproteins as well as the significant enzymatic catalysis and gold nanoparticle signal amplification, and avoids the cell pretreatment and labelling process. This would contribute to the glycomic analysis and the understanding of complex native glycan-related biological processes.

  18. Astrometric sky survey of the zone +2° – +5.5° with the telescope MAC

    Science.gov (United States)

    Lazorenko, P.; Karbovsky, V.; Svachiy, L.; Buromsky, M.; Kasyan, S.

    2016-06-01

    We describe the results of the astrometric sky survey with the telescope MAC which was performed in 2010–2014 by the Main Astronomical observatory of NAS of Ukraine and Astronomical observatory of Taras Shevchenko Kiev national University. We obtained about 6 million of images of the sky objects to 17m in equatorial zone δ = +2°÷+5.5°. All images were obtained during 188 night observational series with use of V-band filter. Now we obtained the preliminary version of KMAC2.0 catalogue. We estimate that precision of positions for bright V<14m stars is 50–90 milliarcsecond and for fainter 14m

  19. Gaia’s Cepheids and RR Lyrae stars and luminosity calibrations based on Tycho-Gaia Astrometric Solution

    Directory of Open Access Journals (Sweden)

    Clementini Gisella

    2017-01-01

    Full Text Available Gaia Data Release 1 contains parallaxes for more than 700 Galactic Cepheids and RR Lyrae stars, computed as part of the Tycho-Gaia Astrometric Solution (TGAS. We have used TGAS parallaxes, along with literature (V, I, J, Ks, W1 photometry and spectroscopy, to calibrate the zero point of the period-luminosity and period-Wesenheit relations of classical and type II Cepheids, and the near-infrared period-luminosity, period-luminosity-metallicity and optical luminosity-metallicity relations of RR Lyrae stars. In this contribution we briefly summarise results obtained by fitting these basic relations adopting different techniques that operate either in parallax or distance (absolute magnitude space.

  20. Simulation and analysis of sub-{\\mu}as precision astrometric data for planet-finding

    CERN Document Server

    Savransky, Dmitry

    2010-01-01

    We present a vector formulation of an interferometric observation of a star, including the effects of the barycentric motion of the observatory, the proper motions of the star, and the reflex motions of the star due to orbiting planets. We use this model to empirically determine the magnitude and form of the signal due to a single Earth-mass planet orbiting about a sun-mass star. Using bounding values for the known components of the model, we perform a series of expansions, comparing the residuals to this signal. We demonstrate why commonly used first order linearizations of similar measurement models are insufficient for signals of the magnitude of the one due to an Earth-mass planet, and present a consistent expansion which is linear in the unknown quantities, with residuals multiple orders of magnitude below the Earth-mass planet signal. We also discuss numerical issues that can arise when simulating or analyzing these measurements.

  1. Signal profile on Gd-EOB-DTPA-enhanced MR imaging in non-alcoholic steatohepatitis and liver cirrhosis induced in rats: correlation with transporter expression

    Energy Technology Data Exchange (ETDEWEB)

    Tsuda, Natsuko [Diagnostic Imaging Medical Affairs, Medical Affairs, Bayer Yakuhin, Ltd., Osaka, Osaka (Japan); Matsui, Osamu [Kanazawa University Graduate School of Medical Science, Department of Radiology, Kanazawa, Ishikawa (Japan)

    2011-12-15

    To compare the transporter expression and signal profile on Gd-EOB-DTPA-enhanced MRI between non-alcoholic steatohepatitis (NASH) and cirrhotic liver induced in rats, and investigate the correlation of the transporter expression and fibrosis rate in both diseases. Forty-eight rats were divided into four groups of 12: TAA (cirrhosis), NASH 7- and 10-week, and control groups. Each group was divided into two subgroups: Group 1 for MRI and Group 2 for transporter examinations. The relative enhancement of the TAA group was significantly lower than those of other groups (p < 0.01). The T{sub max} and T{sub 1/2} of the NASH 10-week group was significantly prolonged in comparison with the TAA group (p < 0.01). There was no significant difference in the oatp1 expression, whereas the mrp2 expression of the TAA group was significantly higher than those of other groups (p < 0.01). There was no significant correlation between the fibrosis rate and oatp1 expression, whereas a paradoxical correlation was found between the fibrosis rate and mrp2 expression (NASH: negative correlation, r = 0.91, p < 0.01; TAA: positive correlation, r = 0.85, p < 0.01). Our findings showed that the mrp2 expression in cirrhosis increases in comparison with NASH, and there was a paradoxical correlation between the fibrosis rate and mrp2 expression. (orig.)

  2. Effect of alpha and Gaussian refractive index profiles on the design of highly nonlinear optical fibre for efficient nonlinear optical signal processing

    Science.gov (United States)

    Selvendran, S.; Sivanantharaja, A.; Arivazhagan, S.; Kannan, M.

    2016-09-01

    We propose an index profiled, highly nonlinear ultraflattened dispersion fibre (HN-UFF) with appreciable values of fibre parameters such as dispersion, dispersion slope, effective area, nonlinearity, bending loss and splice loss. The designed fibre has normal zero flattened dispersion over S, C, L, U bands and extends up to 1.9857 μm. The maximum dispersion variation observed for this fibre is as low as 1.61 ps km-1 nm-1 over the 500-nm optical fibre transmission spectrum. This fibre also has two zero dispersion wavelengths at 1.487 and 1.9857 μm and the respective dispersion slopes are 0.02476 and 0.0068 ps nm-2 km-1. The fibre has a very low ITU-T cutoff wavelength of 1.2613 μm and a virtuous nonlinear coefficient of 9.43 W-1 km-1. The wide spectrum of zero flattened dispersion and a good nonlinear coefficient make the designed fibre very promising for different nonlinear optical signal processing applications.

  3. Analysis of miRNA profiles identified miR-196a as a crucial mediator of aberrant PI3K/AKT signaling in lung cancer cells.

    Science.gov (United States)

    Guerriero, Ilaria; D'Angelo, Daniela; Pallante, Pierlorenzo; Santos, Mafalda; Scrima, Marianna; Malanga, Donatella; De Marco, Carmela; Ravo, Maria; Weisz, Alessandro; Laudanna, Carmelo; Ceccarelli, Michele; Falco, Geppino; Rizzuto, Antonia; Viglietto, Giuseppe

    2016-11-17

    Hyperactivation of the PI3K/AKT pathway is observed in most human cancer including lung carcinomas. Here we have investigated the role of miRNAs as downstream targets of activated PI3K/AKT signaling in Non Small Cell Lung Cancer (NSCLC). To this aim, miRNA profiling was performed in human lung epithelial cells (BEAS-2B) expressing active AKT1 (BEAS-AKT1-E17K), active PI3KCA (BEAS-PIK3CA-E545K) or with silenced PTEN (BEAS-shPTEN).Twenty-four differentially expressed miRNAs common to BEAS-AKT1-E17K, BEAS-PIK3CA-E545K and BEAS-shPTEN cells were identified through this analysis, with miR-196a being the most consistently up-regulated miRNA. Interestingly, miR-196a was significantly overexpressed also in human NSCLC-derived cell lines (n=11) and primary lung cancer samples (n=28).By manipulating the expression of miR-196a in BEAS-2B and NCI-H460 cells, we obtained compelling evidence that this miRNA acts downstream the PI3K/AKT pathway, mediating some of the proliferative, pro-migratory and tumorigenic activity that this pathway exerts in lung epithelial cells, possibly through the regulation of FoxO1, CDKN1B (hereafter p27) and HOXA9.

  4. Monitoring vegetation recovery in fire-affected areas using temporal profiles of spectral signal from time series MODIS and LANDSAT satellite images

    Science.gov (United States)

    Georgopoulou, Danai; Koutsias, Nikos

    2015-04-01

    Vegetation phenology is an important element of vegetation characteristics that can be useful in vegetation monitoring especially when satellite remote sensing observations are used. In that sense temporal profiles extracted from spectral signal of time series MODIS and LANDSAT satellite images can be used to characterize vegetation phenology and thus to be helpful for monitoring vegetation recovery in fire-affected areas. The aim of this study is to explore the vegetation recovery pattern of the catastrophic wildfires that occurred in Peloponnisos, southern Greece, in 2007. These fires caused the loss of 67 lives and were recognized as the most extreme natural disaster in the country's recent history. Satellite remote sensing data from MODIS and LANDSAT satellites in the period from 2000 to 2014 were acquired and processed to extract the temporal profiles of the spectral signal for selected areas within the fire-affected areas. This dataset and time period analyzed together with the time that these fires occurred gave the opportunity to create temporal profiles seven years before and seven years after the fire. The different scale of the data used gave us the chance to understand how vegetation phenology and therefore the recovery patterns are influenced by the spatial resolution of the satellite data used. Different metrics linked to key phenological events have been created and used to assess vegetation recovery in the fire-affected areas. Our analysis was focused in the main land cover types that were mostly affected by the 2007 wildland fires. Based on CORINE land-cover maps these were agricultural lands highly interspersed with large areas of natural vegetation followed by sclerophyllous vegetation, transitional woodland shrubs, complex cultivation patterns and olive groves. Apart of the use of the original spectral data we estimated and used vegetation indices commonly found in vegetation studies as well as in burned area mapping studies. In this study we

  5. BDE-47 causes developmental retardation with down-regulated expression profiles of ecdysteroid signaling pathway-involved nuclear receptor (NR) genes in the copepod Tigriopus japonicus

    Energy Technology Data Exchange (ETDEWEB)

    Hwang, Dae-Sik; Han, Jeonghoon; Won, Eun-Ji; Kim, Duck-Hyun; Jeong, Chang-Bum [Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419 (Korea, Republic of); Hwang, Un-Ki [Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Fisheries Research & Development Institute, Incheon 46083 (Korea, Republic of); Zhou, Bingsheng [State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072 (China); Choe, Joonho [Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141 (Korea, Republic of); Lee, Jae-Seong, E-mail: jslee2@skku.edu [Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419 (Korea, Republic of)

    2016-08-15

    Highlights: • The developmental rate was significantly inhibited (P < 0.05) in response to BDE-47. • Expression profiles of nearly all NR genes were the highest at naupliar stages 5–6. • USP, HR96, and FTZ-F1 genes showed significant sex differences (P < 0.05) over different developmental stages. • NR gene expression patterns showed significant decreases (P<0.05) in response to BDE-47. • BDE-47 leads to molting and metamorphosis retardation and suppresses transcription of NR genes. - Abstract: 2,2′,4,4′-Tetrabromodiphenyl ether (BDE-47) is a persistent organic pollutant (POP) in marine environments. Despite its adverse effects (e.g. developmental retardation) in ecdysozoa, the effects of BDE-47 on transcription of ecdysteroid signaling pathway-involved-nuclear receptor (NR) genes and metamorphosis-related genes have not been examined in copepods. To examine the deleterious effect of BDE-47 on copepod molting and metamorphosis, BDE-47 was exposed to the harpacticoid copepod Tigriopus japonicus, followed by monitoring developmental retardation and transcriptional alteration of NR genes. The developmental rate was significantly inhibited (P < 0.05) in response to BDE-47 and the agricultural insecticide gamma-hexachlorocyclohexane. Conversely, the ecdysteroid agonist ponasterone A (PoA) led to decreased molting and metamorphosis time (P < 0.05) from the nauplius stage to the adult stage. In particular, expression profiles of all NR genes were the highest at naupliar stages 5–6 except for SVP, FTZ-F1, and HR96 genes. Nuclear receptor USP, HR96, and FTZ-F1 genes also showed significant sex differences (P < 0.05) in gene expression levels over different developmental stages, indicating that these genes may be involved in vitellogenesis. NR gene expression patterns showed significant decreases (P < 0.05) in response to BDE-47 exposure, implying that molting and metamorphosis retardation is likely associated with NR gene expression. In summary, BDE-47

  6. Expression profile of mitrogen-activated protein kinase (MAPK signaling genes in the skeletal muscle & liver of rat with type 2 diabetes: Role in disease pathology

    Directory of Open Access Journals (Sweden)

    Xiaoli Tang

    2014-01-01

    Full Text Available Background & objectives: Type 2 diabetes (T2D is characterized as hyperglycaemia caused by defects in insulin secretion, and it affects target tissues, such as skeletal muscle, liver and adipose tissue. Therefore, analyzing the changes of gene expression profiles in these tissues is important to elucidate the pathogenesis of T2D. We, therefore, measured the gene transcript alterations in liver and skeletal muscle of rat with induced T2D, to detect differentially expressed genes in liver and skeletal muscle and perform gene-annotation enrichment analysis. Methods: In the present study, skeletal muscle and liver tissue from 10 streptozotocin-induced diabetic rats and 10 control rats were analyzed using gene expression microarrays. KEGG pathways enriched by differentially expressed genes (DEGs were identified by WebGestalt Expander and GATHER software. DEGs were validated by the method of real-time PCR and western blot. Results: From the 9,929 expressed genes across the genome, 1,305 and 997 differentially expressed genes (DEGs, P<0.01 were identified in comparisons of skeletal muscle and liver, respectively. Large numbers of DEGs (200 were common in both comparisons, which was clearly more than the predicted number (131 genes, P<0.001. For further interpretation of the gene expression data, three over-representation analysis softwares (WebGestalt, Expander and GATHER were used. All the tools detected one KEGG pathway (MAPK signaling and two GO (gene ontology biological processes (response to stress and cell death, with enrichment of DEGs in both tissues. In addition, PPI (protein-protein interaction networks constructed using human homologues not only revealed the tendency of DEGs to form a highly connected module, but also suggested a "hub" role of p38-MAPK-related genes (such as MAPK14 in the pathogenesis of T2D. Interpretation & conclusions: Our results indicated the considerably aberrant MAPK signaling in both insulin-sensitive tissues of T2D

  7. DNA chip-based expression profile analysis indicates involvement of the phosphatidylinositol signaling pathway in multiple plant responses to hormone and abiotic treatments

    Institute of Scientific and Technical Information of China (English)

    Wen Hui LIN; Rui YE; Hui MA; Zhi Hong XU; Hong Wei XUE

    2004-01-01

    The phosphatidylinositol (PI) metabolic pathway is considered critical in plant responses to many environmental factors,and previous studies have indicated the involvement of multiple PI-related gene families during cellular responses.Through a detailed analysis of the Arabidopsis thaliana genome,82 polypeptides were identified as being involved in PI signaling. These could be grouped into different families including PI synthases (PIS),PI-phosphate kinases (PIPK),phospholipases (PL),inositol polyphosphate phosphatases (IPPase),inositol polyphosphate kinases (IPK),PI transfer proteins and putative inositol polyphosphate receptors. The presence of more than 10 isoforms of PIPK,PLC,PLD and IPPase suggested that these genes might be differentially expressed during plant cellular responses or growth and development. Accordingly,DNA chip technology was employed to study the expression patterns of various isoforms.In total,79 mRNA clones were amplified and used for DNA chip generation. Expression profile analysis was performed using samples that represented multiple tissues or cellular responses. Tested samples included normal leaf,stem and flower tissues,and leaves from plants treated with various hormones (auxin,cytokinin,gibberellin,abscisic acid and brassinosteroid) or environmental factors (temperature,calcium,sodium,drought,salicylic acid and jasmonic acid).Results showed that many PI pathway-related genes were differentially expressed under these experimental conditions.In particular,the different isoforms of each family were specifically expressed in many cases,suggesting their involvement in tissue specificity and cellular responses to environmental conditions. This work provides a starting point for functional studies of the relevant PI-related proteins and may help shed light onto the role of PI pathways in development and cellular responses.

  8. An astrometric and spectroscopic study of the δ Scuti variable HD 21190 and its wide companion CPD -83° 64B

    Science.gov (United States)

    Niemczura, E.; Scholz, R.-D.; Hubrig, S.; Järvinen, S. P.; Schöller, M.; Ilyin, I.; Kahraman Aliçavuş, F.

    2017-10-01

    Although pulsations of δ Scuti type are not expected among Ap stars from a theoretical point of view, previous observations of the known δ Scuti star HD 21190 indicated a spectral classification F2 III SrEuSi:, making it the most evolved Ap star known. Our atmospheric chemical analysis based on recent High Accuracy Radial velocity Planet Searcher observations confirms the presence of chemical peculiarities in HD 21190. This star is also the only target known to host a magnetic field along with its δ Scuti pulsation properties. Using an astrometric analysis, we show that HD 21190 forms a physical binary system with the companion CPD -83° 64B. The presented astrometric and spectroscopic study of the binary components is important to understand the complex interplay between stellar pulsations, magnetic fields and chemical composition.

  9. M2000 an astrometric catalog in the Bordeaux Carte du Ciel zone +11 degrees < {delta} < +18 degrees

    CERN Document Server

    Rapaport, M S; Soubiran, C; Daigne, G; Perié, J P; Bosq, F; Colin, J; Desbats, J M; Ducourant, C; Mazurier, J M; Montignac, G; Ralite, N; Réquième, Y; Viateau, B

    2001-01-01

    During four years, systematic observations have been conducted in drift scan mode with the Bordeaux automated meridian circle in the declination band [+11 ; +18]. The resulting astrometric catalog includes about 2 300 000 stars down to the magnitude limit V_M=16.3. Nearly all stars (96%) have been observed at least 6 times, the catalog being complete down to V_M=15.4. The median internal standard error in position is about 35 mas in the V_M magnitude range [11 ; 15], which degrades to about 50 mas when the faintest stars are considered. M2000 provides also one band photometry with a median internal standard error of 0.04 mag. Comparisons with the Hipparcos and bright part of Tycho-2 catalogs have enabled to estimate external errors in position to be lower than 40 mas. In this zone and at epoch 1998, the faint part of Tycho-2 is found to have an accuracy of 116 mas in alpha instead of 82 mas deduced from the model-based standard errors given in the catalog.

  10. The Space Interferometry Mission Astrometric Grid Giant-Star Survey. I. Stellar Parameters and Radial Velocity Variability

    CERN Document Server

    Bizyaev, D; Cunha, K; Geisler, D; Gieren, W; Majewski, S R; Pardo, C D; Patterson, R J; Smith, V V; Suntzeff, N B; Arenas, Jose; Bizyaev, Dmitry; Cunha, Katia; Geisler, Doug; Gieren, Wolfgang; Majewski, Steven R.; Pardo, Cecilia Del; Patterson, Richard J.; Smith, Verne V.; Suntzeff, Nicholas B.

    2005-01-01

    We present results from a campaign of multiple epoch echelle spectroscopy of relatively faint (V = 9.5-13.5 mag) red giants observed as potential astrometric grid stars for the Space Interferometry Mission (SIM PlanetQuest). Data are analyzed for 775 stars selected from the Grid Giant Star Survey spanning a wide range of effective temperatures (Teff), gravities and metallicities. The spectra are used to determine these stellar parameters and to monitor radial velocity (RV) variability at the 100 m/s level. The degree of RV variation measured for 489 stars observed two or more times is explored as a function of the inferred stellar parameters. The percentage of radial velocity unstable stars is found to be very high -- about 2/3 of our sample. It is found that the fraction of RV-stable red giants (at the 100 m/s level) is higher among stars with Teff \\sim 4500 K, corresponding to the calibration-independent range of infrared colors 0.59 < (J-K_s)_0 < 0.73. A higher percentage of RV-stable stars is found ...

  11. Wind Profiling Radar

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Clutter present in radar return signals as used for wind profiling is substantially removed by carrying out a Daubechies wavelet transformation on a time series of...

  12. 天体测量法探测系外行星∗%Exoplanet Detection by Astrometric Method

    Institute of Scientific and Technical Information of China (English)

    许伟维; 廖新浩; 周永宏; 许雪晴

    2016-01-01

    As we known, the exoplanets are mostly detected by the methods of radial velocity and transit, only one is found by the astrometric method. As the data of the gaia to be released, astrometry will become one of the most important method for detecting exoplanets gradually. Based on the position sequence of stars, this paper discusses the calculation of the equations of dynamics conditions involved in solving the mass and the orbit parameters of the planet. Due to the deficiency of the available theory (orbital element method), we put forward a new method (coordinate velocity method). The differential correction formulae of the two methods are presented, as well as the necessary simulation. In addition, the method established in this paper can be applied to the multi-planet system easily.%在目前已发现的系外行星中,绝大多数是由视向速度法和凌星法探测得到的,天体测量法仅发现了1颗。 gaia卫星数据即将发布,天体测量法将逐步成为系外行星探测的重要方法之一。基于天体测量法给出的恒星位置参数序列,讨论了在求解行星质量和轨道参数时涉及的动力学条件方程计算问题,给出了具体微分改正公式,同时也进行了必要的仿真模拟计算。建立的方法可以较容易地推广到多行星系统。

  13. In vivo immunotoxicity of perfluorooctane sulfonate in BALB/c mice: Identification of T-cell receptor and calcium-mediated signaling pathway disruption through gene expression profiling of the spleen.

    Science.gov (United States)

    Lv, Qi-Yan; Wan, Bin; Guo, Liang-Hong; Yang, Yu; Ren, Xiao-Min; Zhang, Hui

    2015-10-05

    Perfluorooctane sulfonate (PFOS) is a persistent organic pollutant that is used worldwide and is continuously being detected in biota and the environment, thus presenting potential threats to the ecosystem and human health. Although PFOS is highly immunotoxic, its underlying molecular mechanisms remain largely unknown. The present study examined PFOS-induced immunotoxicity in the mouse spleen and explored its underlying mechanisms by gene expression profiling. Oral exposure of male BALB/c mice for three weeks followed by one-week recovery showed that a 10 mg/kg/day PFOS exposure damaged the splenic architecture, inhibited T-cell proliferation in response to mitogen, and increased the percentages of T helper (CD3(+)CD4(+)) and cytotoxic T (CD3(+)CD8(+)) cells, despite the decrease in the absolute number of these cells. A delayed type of PFOS immunotoxicity was observed, which mainly occurred during the recovery period. Global gene expression profiling of mouse spleens and QRT-PCR analyses suggest that PFOS inhibited the expression of genes involved in cell cycle regulation and NRF2-mediated oxidative stress response, and upregulated those in TCR signaling, calcium signaling, and p38/MAPK signaling pathways. Western blot analysis confirmed that the expressions of CAMK4, THEMIS, and CD3G, which were involved in the upregulated pathways, were induced upon PFOS exposure. Acute PFOS exposure modulated calcium homoeostasis in splenocytes. These results indicate that PFOS exposure can activate TCR signaling and calcium ion influx, which provides a clue for the potential mechanism of PFOS immunotoxicity. The altered signaling pathways by PFOS treatment as revealed in the present study might facilitate in better understanding PFOS immunotoxicity and explain the association between immune disease and PFOS exposure.

  14. Combining ChIP-chip and expression profiling to model the MoCRZ1 mediated circuit for Ca/calcineurin signaling in the rice blast fungus.

    Directory of Open Access Journals (Sweden)

    Soonok Kim

    2010-05-01

    Full Text Available Significant progress has been made in defining the central signaling networks in many organisms, but collectively we know little about the downstream targets of these networks and the genes they regulate. To reconstruct the regulatory circuit of calcineurin signal transduction via MoCRZ1, a Magnaporthe oryzae C2H2 transcription factor activated by calcineurin dephosphorylation, we used a combined approach of chromatin immunoprecipitation - chip (ChIP-chip, coupled with microarray expression studies. One hundred forty genes were identified as being both a direct target of MoCRZ1 and having expression concurrently differentially regulated in a calcium/calcineurin/MoCRZ1 dependent manner. Highly represented were genes involved in calcium signaling, small molecule transport, ion homeostasis, cell wall synthesis/maintenance, and fungal virulence. Of particular note, genes involved in vesicle mediated secretion necessary for establishing host associations, were also found. MoCRZ1 itself was a target, suggesting a previously unreported autoregulation control point. The data also implicated a previously unreported feedback regulation mechanism of calcineurin activity. We propose that calcium/calcineurin regulated signal transduction circuits controlling development and pathogenicity manifest through multiple layers of regulation. We present results from the ChIP-chip and expression analysis along with a refined model of calcium/calcineurin signaling in this important plant pathogen.

  15. High precision astrometry mission for the detection and characterization of nearby habitable planetary systems with the Nearby Earth Astrometric Telescope (NEAT)

    CERN Document Server

    Malbet, Fabien; Shao, Michael; Goullioud, Renaud; Lagage, Pierre-Olivier; Brown, Anthony G A; Cara, Christophe; Durand, Gilles; Eiroa, Carlos; Feautrier, Philippe; Jakobsson, Björn; Hinglais, Emmanuel; Kaltenegger, Lisa; Labadie, Lucas; Lagrange, Anne-Marie; Laskar, Jacques; Liseau, René; Lunine, Jonathan; Maldonado, Jesús; Mercier, Manuel; Mordasini, Christoph; Queloz, Didier; Quirrenbach, Andreas; Sozzetti, Alessandro; Traub, Wesley; Absil, Olivier; Alibert, Yann; Andrei, Alexandre Humberto; Beichman, Charles; Chelli, Alain; Cockell, Charles S; Duvert, Gilles; Forveille, Thierry; Garcia, Paulo J V; Hobbs, David; Krone-Martins, Alberto; Lammer, Helmut; Meunier, Nadège; Minardi, Stefano; de Almeida, André Moitinho; Rambaux, Nicolas; Raymond, Sean; Röttgering, Huub J A; Sahlmann, Johannes; Schuller, Peter A; Ségransan, Damien; Selsis, Franck; Surdej, Jean; Villaver, Eva; White, Glenn J; Zinnecker, Hans

    2011-01-01

    (abridged) A complete census of planetary systems around a volume-limited sample of solar-type stars (FGK dwarfs) in the Solar neighborhood with uniform sensitivity down to Earth-mass planets within their Habitable Zones out to several AUs would be a major milestone in extrasolar planets astrophysics. This fundamental goal can be achieved with a mission concept such as NEAT - the Nearby Earth Astrometric Telescope. NEAT is designed to carry out space-borne extremely-high-precision astrometric measurements sufficient to detect dynamical effects due to orbiting planets of mass even lower than Earth's around the nearest stars. Such a survey mission would provide the actual planetary masses and the full orbital geometry for all the components of the detected planetary systems down to the Earth-mass limit. The NEAT performance limits can be achieved by carrying out differential astrometry between the targets and a set of suitable reference stars in the field. The NEAT instrument design consists of an off-axis para...

  16. ASTROMETRIC CATALOGS: BE CAREFUL!

    Directory of Open Access Journals (Sweden)

    R. Teixeira

    2014-01-01

    Full Text Available En este trabajo hemos inicado una investigaci ́n sobre los movimientos propios en los principales –y extensos– o cat ́logos astrom ́tricos. Esta investigaci ́n consiste en la comparaci ́n de los movimientos propios de tres a e o o cat ́logos con los obtenidos exclusivamente mediante observaciones CCD con el c ́ a ırculo meridiano hechas en el Observatorio Abrah ̃o de Moraes, Valinhos (Brasil durante 15 a ̃os. La motivaci ́n para este trabajo es el uso a n o –c ́modo pero peligroso– de los movimientos propios de los m ́s importantes cat ́logos, que globalmente son o a a muy buenos, pero que localmente no lo son. Nuestro prop ́sito es llamar la atenci ́n sobre la necesidad de usar o o estos cat ́logos con cuidado, sobre todo para los movimientos propios de las estrellas d ́biles.

  17. Gamma Astrometric Measurement Experiment

    Science.gov (United States)

    Gai, M.; Lattanzi, M. G.; Ligori, S.; Loreggia, D.; Vecchiato, A.

    GAME aims at the measurement of gravitational deflection of the light by the Sun, by an optimised telescope on board a small class satellite. The targeted precision on the gamma parameter of the Parametrised Post-Newtonian formulation of General Relativity is below 10-6, i.e. one to two orders of magnitude better than the best current results. Such precision is suitable to detect possible deviations from the unity value, associated to generalised Einstein models for gravitation, with potentially huge impacts on the cosmological distribution of dark matter and dark energy. The measurement principle is based on differential astrometry. The observations also allow additional scientific objectives related to tests of General Relativity and to the study of exo-planetary systems. The instrument concept is based on a dual field, multiple aperture Fizeau interferometer, observing simultaneously two regions close to the Solar limb. The diluted optics achieves efficient rejection of the solar radiation, with good angular resolution on the science targets. We describe the science motivation, the proposed mission implementation and the expected performance.

  18. Measurement of signal intensity depth profiles in rat brains with cardiac arrest maintaining primary temperature by wide-field optical coherence tomography.

    Science.gov (United States)

    Sato, Manabu; Nomura, Daisuke; Tsunenari, Takashi; Nishidate, Izumi

    2010-09-10

    We have already reported that after an injection for euthanasia, the signal intensity of optical coherence tomography (OCT) images are 2.7 times increased before cardiac arrest (CA) using OCT and rat brains without temperature control to show the potential of OCT to monitor tissue viability in brains [Appl. Opt.48, 4354 (2009)APOPAI0003-693510.1364/AO.48.004354]. In this paper, we similarly measured maintaining the primary temperature of rat brains. It was confirmed that when maintaining the primary temperature, the time courses of the ratios of signal intensity (RSIs) were almost the same as those without temperature control. RSIs after CA varied from 1.6 to 4.5 and depended on positions measured in tissues. These results mean that the OCT technique has clinical potential for applications to monitor or diagnose a focal degraded area, such as cerebral infarctions due to focal ischemia in brains.

  19. Multifaceted role of nitric oxide in an in vitro mouse neuronal injury model: transcriptomic profiling defines the temporal recruitment of death signalling cascades

    Science.gov (United States)

    Peng, Zhao Feng; Chen, Minghui Jessica; Manikandan, Jayapal; Melendez, Alirio J; Shui, Guanghou; Russo-Marie, Françoise; Whiteman, Matthew; Beart, Philip M; Moore, Philip K; Cheung, Nam Sang

    2012-01-01

    Abstract Nitric oxide is implicated in the pathogenesis of various neuropathologies characterized by oxidative stress. Although nitric oxide has been reported to be involved in the exacerbation of oxidative stress observed in several neuropathologies, existent data fail to provide a holistic description of how nitrergic pathobiology elicits neuronal injury. Here we provide a comprehensive description of mechanisms contributing to nitric oxide induced neuronal injury by global transcriptomic profiling. Microarray analyses were undertaken on RNA from murine primary cortical neurons treated with the nitric oxide generator DETA-NONOate (NOC-18, 0.5 mM) for 8–24 hrs. Biological pathway analysis focused upon 3672 gene probes which demonstrated at least a ±1.5-fold expression in a minimum of one out of three time-points and passed statistical analysis (one-way anova, P < 0.05). Numerous enriched processes potentially determining nitric oxide mediated neuronal injury were identified from the transcriptomic profile: cell death, developmental growth and survival, cell cycle, calcium ion homeostasis, endoplasmic reticulum stress, oxidative stress, mitochondrial homeostasis, ubiquitin-mediated proteolysis, and GSH and nitric oxide metabolism. Our detailed time-course study of nitric oxide induced neuronal injury allowed us to provide the first time a holistic description of the temporal sequence of cellular events contributing to nitrergic injury. These data form a foundation for the development of screening platforms and define targets for intervention in nitric oxide neuropathologies where nitric oxide mediated injury is causative. PMID:21352476

  20. Quorum sensing signal profile of Acinetobacter strains from nosocomial and environmental sources Perfil de sensores de quórum en cepas nosocomiales y ambientales de Acinetobacter

    Directory of Open Access Journals (Sweden)

    R. H. González

    2009-06-01

    Full Text Available A set of 43 strains corresponding to 20 classified and unclassified genomic Acinetobacter species was analyzed for the production of typical N-acyl homoserine lactone quorum sensing molecules in culture broths. A large percentage of the strains (74% displayed quorum sensing signals that could be separated into three statistically significantly different chromatographic groups (p Rf2 > Rf1. None of the three signals could be specifically assigned to a particular species in the genus; furthermore, no distinction could be made between the quorum sensing signals secreted by typical opportunistic strains of the A. calcoaceticus-A. baumannii complex, isolated from patients, with respect to the other species of the genus, except for the Rf1 signal which was present in all the QS positive strains belonging to this complex and DNA group 13 TU. In conclusion, quorum sensors in Acinetobacter are not homogenously distributed among species and one of them is present in most of the A. calcoaceticus-baumannii complex.Se analizó la producción de moléculas típicas de N-acil homoserina lactona con actividad de quorum sensing en cultivos líquidos de un grupo de 43 cepas correspondientes a 20 especies genómicas clasificadas y no clasificadas de Acinetobacter. Un porcentaje alto de las cepas (74% mostraron señales de quorum sensing que pudieron ser separadas en tres grupos cromatográficos significativamente diferentes entre sí (p Rf2 > Rf1. Ninguna de las tres señales pudo ser asignada a una especie en particular dentro del género; es más, no se encontró diferencia entre las señales producidas por las cepas típicamente oportunistas (complejo A. calcoaceticus-A. baumannii aisladas de pacientes respecto de las producidas por otras cepas del mismo género, excepto para el caso de Rf1, que se encontró presente en todos los aislamientos quorum sensing positivos del mencionado complejo y en las cepas del grupo de DNA 13TU. En conclusión, los sensores de

  1. Chronic vagus nerve stimulation attenuates vascular endothelial impairments and reduces the inflammatory profile via inhibition of the NF-κB signaling pathway in ovariectomized rats.

    Science.gov (United States)

    Li, Ping; Liu, Huaipu; Sun, Peng; Wang, Xiaoyu; Wang, Chen; Wang, Ling; Wang, Tinghuai

    2016-02-01

    Vagus nerve stimulation (VNS), a method for activating cholinergic anti-inflammatory pathways, could suppress endothelial activation and minimize tissue injury during inflammation. The aim of this study was to investigate the effects of chronic VNS on endothelial impairments and the inflammatory profile in ovariectomized (OVX) rats. Sprague-Dawley rats (7-8 months old) were randomly assigned to the following four groups: sham-OVX, OVX, OVX+sham-VNS, and OVX+VNS. Throughout the experimental period, the OVX+VNS group received VNS for 3h (20.0 Hz, 1.0 mA, and 10.00 ms pulse width) at the same time every other day. After 12 weeks of VNS, blood samples and thoracic aortas were collected for further analyses. Light microscopy and electron microscopy analyses showed that chronic VNS prevented endothelial swelling, desquamation and even necrosis in the OVX rats. In addition, it obviously improved endothelial function in the OVX rats by restoring the endothelial nitric oxide synthase (e-NOS) and serum endothelin-1 level. Increased expression of cell adhesion molecules (VCAM-1, ICAM-1 and E-selectin) in the thoracic aortas and increases in the levels of circulating cytokines (TNF-α, IL-6, MCP-1, and CINC/KC) were also observed in the OVX rats. Chronic VNS significantly restored these detrimental changes partly by increasing the ACh concentrations in vascular walls and blocking NF-κB pathway activity. The results of this in vivo study have shown that the administration of chronic VNS during, in the early stage of estrogen deficiency, protects OVX rats from endothelial impairments and the inflammatory profile. These findings indicate that activation of the vagus nerve could be a promising supplemental therapy for reducing the risks of suffering from further CVDs in postmenopausal women.

  2. Profiling signalling pathways in formalin-fixed and paraffin-embedded breast cancer tissues reveals cross-talk between EGFR, HER2, HER3 and uPAR.

    Science.gov (United States)

    Berg, Daniela; Wolff, Claudia; Malinowsky, Katharina; Tran, Kai; Walch, Axel; Bronger, Holger; Schuster, Tibor; Höfler, Heinz; Becker, Karl-Friedrich

    2012-01-01

    In the last few years, new approaches and developments in patient-tailored cancer therapies have raised the need to select, more precisely, those patients who will respond to personalized treatments. Therefore, the most efficient way for optimal therapy and patient selection is to provide a tumour-specific protein network portrait prior to treatment. The aim of our study was to monitor protein networks in formalin-fixed and paraffin-embedded (FFPE) breast cancer tissues, with special emphasis on epidermal growth factor receptor 2 (HER2)-mediated signalling pathways, to identify and validate new disease markers. For this purpose we used a recently developed technology to extract full-length proteins from FFPE tissues and analysed 23 molecules involved in HER2-related signalling by reverse phase protein microarray (RPPA) in a series of 106 FFPE breast cancer tissue samples. We found a significant correlation of HER2 with human epidermal growth factor receptor 3 (HER3/erbB3), epidermal growth factor receptor 1 (EGFR/HER1/erbB1) and urokinase plasminogen receptor (uPAR) in routinely used FFPE breast cancer tissues. Thus, targeting HER2, EGFR, HER3 and uPAR together may offer a more efficient treatment option for patients with breast cancer.

  3. Molecular cloning, transcriptional profiling, and subcellular localization of signal transducer and activator of transcription 2 (STAT2) ortholog from rock bream, Oplegnathus fasciatus.

    Science.gov (United States)

    Bathige, S D N K; Umasuthan, Navaneethaiyer; Priyathilaka, Thanthrige Thiunuwan; Thulasitha, William Shanthakumar; Jayasinghe, J D H E; Wan, Qiang; Nam, Bo-Hye; Lee, Jehee

    2017-08-30

    Signal transducer and activator of transcription 2 (STAT2) is a key element that transduces signals from the cell membrane to the nucleus via the type I interferon-signaling pathway. Although the structural and functional aspects of STAT proteins are well studied in mammals, information on teleostean STATs is very limited. In this study, a STAT paralog, which is highly homologous to the STAT2 members, was identified from a commercially important fish species called rock bream and designated as RbSTAT2. The RbSTAT2 gene was characterized at complementary DNA (cDNA) and genomic sequence levels, and was found to possess structural features common with its mammalian counterparts. The complete cDNA sequence was distributed into 24 exons in the genomic sequence. The promoter proximal region was analyzed and found to contain potential transcription factor binding sites to regulate the transcription of RbSTAT2. Phylogenetic studies and comparative genomic structure organization revealed the distinguishable evolution for fish and other vertebrate STAT2 orthologs. Transcriptional quantification was performed by SYBR Green quantitative real-time PCR (qPCR) and the ubiquitous expression of RbSTAT2 transcripts was observed in all tissues analyzed from healthy fish, with a remarkably high expression in blood cells. Significantly (Prock bream irido virus; RBIV), bacterial (Edwardsiella tarda and Streptococcus iniae), and immune stimulants (poly I:C and LPS). Antiviral potential was further confirmed by WST-1 assay, by measuring the viability of rock bream heart cells treated with RBIV. In addition, results of an in vitro challenge experiment signified the influence of rock bream interleukin-10 (RbIL-10) on transcription of RbSTAT2. Subcellular localization studies by transfection of pEGFP-N1/RbSTAT2 into rock bream heart cells revealed that the RbSTAT2 was usually located in the cytoplasm and translocated near to the nucleus upon poly I:C administration. Altogether, these

  4. Combined genome-wide expression profiling and targeted RNA interference in primary mouse macrophages reveals perturbation of transcriptional networks associated with interferon signalling

    Directory of Open Access Journals (Sweden)

    Craigon Marie

    2009-08-01

    Full Text Available Abstract Background Interferons (IFNs are potent antiviral cytokines capable of reprogramming the macrophage phenotype through the induction of interferon-stimulated genes (ISGs. Here we have used targeted RNA interference to suppress the expression of a number of key genes associated with IFN signalling in murine macrophages prior to stimulation with interferon-gamma. Genome-wide changes in transcript abundance caused by siRNA activity were measured using exon-level microarrays in the presence or absence of IFNγ. Results Transfection of murine bone-marrow derived macrophages (BMDMs with a non-targeting (control siRNA and 11 sequence-specific siRNAs was performed using a cationic lipid transfection reagent (Lipofectamine2000 prior to stimulation with IFNγ. Total RNA was harvested from cells and gene expression measured on Affymetrix GeneChip Mouse Exon 1.0 ST Arrays. Network-based analysis of these data revealed six siRNAs to cause a marked shift in the macrophage transcriptome in the presence or absence IFNγ. These six siRNAs targeted the Ifnb1, Irf3, Irf5, Stat1, Stat2 and Nfkb2 transcripts. The perturbation of the transcriptome by the six siRNAs was highly similar in each case and affected the expression of over 600 downstream transcripts. Regulated transcripts were clustered based on co-expression into five major groups corresponding to transcriptional networks associated with the type I and II IFN response, cell cycle regulation, and NF-KB signalling. In addition we have observed a significant non-specific immune stimulation of cells transfected with siRNA using Lipofectamine2000, suggesting use of this reagent in BMDMs, even at low concentrations, is enough to induce a type I IFN response. Conclusion Our results provide evidence that the type I IFN response in murine BMDMs is dependent on Ifnb1, Irf3, Irf5, Stat1, Stat2 and Nfkb2, and that siRNAs targeted to these genes results in perturbation of key transcriptional networks associated

  5. Transcriptional Profiling of Hypoxic Neural Stem Cells Identifies Calcineurin-NFATc4 Signaling as a Major Regulator of Neural Stem Cell Biology.

    Science.gov (United States)

    Moreno, Marta; Fernández, Virginia; Monllau, Josep M; Borrell, Víctor; Lerin, Carles; de la Iglesia, Núria

    2015-08-11

    Neural stem cells (NSCs) reside in a hypoxic microenvironment within the brain. However, the crucial transcription factors (TFs) that regulate NSC biology under physiologic hypoxia are poorly understood. Here we have performed gene set enrichment analysis (GSEA) of microarray datasets from hypoxic versus normoxic NSCs with the aim of identifying pathways and TFs that are activated under oxygen concentrations mimicking normal brain tissue microenvironment. Integration of TF target (TFT) and pathway enrichment analysis identified the calcium-regulated TF NFATc4 as a major candidate to regulate hypoxic NSC functions. Nfatc4 expression was coordinately upregulated by top hypoxia-activated TFs, while NFATc4 target genes were enriched in hypoxic NSCs. Loss-of-function analyses further revealed that the calcineurin-NFATc4 signaling axis acts as a major regulator of NSC self-renewal and proliferation in vitro and in vivo by promoting the expression of TFs, including Id2, that contribute to the maintenance of the NSC state.

  6. Activating FLT3 mutants show distinct gain-of-function phenotypes in vitro and a characteristic signaling pathway profile associated with prognosis in acute myeloid leukemia.

    Directory of Open Access Journals (Sweden)

    Hanna Janke

    Full Text Available About 30% of patients with acute myeloid leukemia (AML harbour mutations of the receptor tyrosine kinase FLT3, mostly internal tandem duplications (ITD and point mutations of the second tyrosine kinase domain (TKD. It was the aim of this study to comprehensively analyze clinical and functional properties of various FLT3 mutants. In 672 normal karyotype AML patients FLT3-ITD, but not FLT3-TKD mutations were associated with a worse relapse free and overall survival in multivariate analysis. In paired diagnosis-relapse samples FLT3-ITD showed higher stability (70% compared to FLT3-TKD (30%. In vitro, FLT3-ITD induced a strong activating phenotype in Ba/F3 cells. In contrast, FLT3-TKD mutations and other point mutations--including two novel mutations--showed a weaker but clear gain-of-function phenotype with gradual increase in proliferation and protection from apoptosis. The pro-proliferative capacity of the investigated FLT3 mutants was associated with cell surface expression and tyrosine 591 phosphorylation of the FLT3 receptor. Western blot experiments revealed STAT5 activation only in FLT3-ITD positive cell lines, in contrast to FLT3-non-ITD mutants, which displayed an enhanced signal of AKT and MAPK activation. Gene expression analysis revealed distinct difference between FLT3-ITD and FLT3-TKD for STAT5 target gene expression as well as deregulation of SOCS2, ENPP2, PRUNE2 and ART3. FLT3-ITD and FLT3 point mutations show a gain-of-function phenotype with distinct signalling properties in vitro. Although poor prognosis in AML is only associated with FLT3-ITD, all activating FLT3 mutations can contribute to leukemogenesis and are thus potential targets for therapeutic interventions.

  7. Defect Profile Reconstruction From Pulsed Eddy Current Signals Based on Radial Basis Function Neural Network%基于径向基神经网络的脉冲涡流缺陷轮廓重构

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    采用脉冲涡流技术进行检测时,为准确得到被测缺陷的轮廓,提出了一种基于径向基神经网络的缺陷轮廓重构方法.该方法为降低网络结构对重构结果的影响,采用主成分分析法对网络隐层应选择的最少节点数进行了计算,进而确定了较合理的网络结构;而后采用混合学习算法求得了网络参数,并通过引入梯度信息衰减系数对求解过程进行了优化;最后将其应用于脉冲涡流检测的缺陷轮廓重构实验,结果表明:基于径向基神经网络的缺陷轮廓重构方法不仅具有较高的重构精度而且具有较强的抗噪声干扰能力,是一种有效可行的脉冲涡流缺陷轮廓重构方法.%In order to obtain the defect profile when the pulsed eddy current testing was used, an approach of defect profile reconstruction from pulsed eddy current signals based on radial basis function neural network( RBFNN) was proposed in this paper. To reduce the effect of network structure on reconstruction result, principal components analysis was used to determine the least number of hidden nodes, then the appropriate network structure was determined. The hybrid learning algorithm was used to solve the parameter of RBFNN, and the solving process was optimized by attenuation coefficient of gradient information. Then the pro-posed approach was utilized in the experiment of defect profile reconstruction, the results indicate that the defect profile can be re-constructed accurately and the performance of noise interference suppression of the method is high, thus it is an effective and feasi-ble approach for pulsed eddy current defect profile reconstruction.

  8. An Astrometric Search for a Sub-stellar Companion of the M8.5 Dwarf TVLM 513-46546 Using Very Long Baseline Interferometry

    CERN Document Server

    Forbrich, Jan; Reid, Mark J

    2013-01-01

    We conducted multi-epoch VLBI observations to search for astrometric reflex motion caused by a sub-stellar companion of the M8.5 dwarf TVLM 513-46546. The observations yield an absolute parallax corresponding to a distance of 10.762+/-0.027 pc and a proper motion of 78.09+/-0.17 mas/yr. From the absence of significant residual motion, we place an upper limit to any reflex motion caused by a companion, extending the parameter space covered by previous near-infrared direct-imaging searches. By covering different orbital periods, the data exclude a phase-space of companion masses and orbital periods ranging from 3.8 Mjup with an orbital radius of ~0.05 AU (orbital period of 16 days) to 0.3 Mjup with an orbital radius of ~0.7 AU (orbital period of 710 days).

  9. Transcript profiling reveals auxin and cytokinin signaling pathways and transcription regulation during in vitro organogenesis of Ramie (Boehmeria nivea L. Gaud.

    Directory of Open Access Journals (Sweden)

    Xing Huang

    Full Text Available In vitro organogenesis, one of the most common pathways leading to in vitro plant regeneration, is widely used in biotechnology and the fundamental study of plant biology. Although previous studies have constructed a complex regulatory network model for Arabidopsis in vitro organogenesis, no related study has been reported in ramie. To generate more complete observations of transcriptome content and dynamics during ramie in vitro organogenesis, we constructed a reference transcriptome library and ten digital gene expression (DGE libraries for illumina sequencing. Approximately 111.34 million clean reads were obtained for transcriptome and the DGE libraries generated between 13.5 and 18.8 million clean reads. De novo assembly produced 43,222 unigenes and a total of 5,760 differentially expressed genes (DEGs were filtered. Searching against the Kyoto Encyclopedia of Genes and Genomes Pathway database, 26 auxin related and 11 cytokinin related DEGs were selected for qRT-PCR validation of two ramie cultivars, which had high (Huazhu No. 5 or extremely low (Dazhuhuangbaima shoot regeneration abilities. The results revealed differing regulation patterns of auxin and cytokinin in different genotypes. Here we report the first genome-wide gene expression profiling of in vitro organogenesis in ramie and provide an overview of transcription and phytohormone regulation during the process. Furthermore, the auxin and cytokinin related genes have distinct expression patterns in two ramie cultivars with high or extremely low shoot regeneration ability, which has given us a better understanding of the in vitro organogenesis mechanism. This result will provide a foundation for future phytohormone research and lead to improvements of the ramie regeneration system.

  10. Transcriptional Profiling of Hypoxic Neural Stem Cells Identifies Calcineurin-NFATc4 Signaling as a Major Regulator of Neural Stem Cell Biology

    Science.gov (United States)

    Moreno, Marta; Fernández, Virginia; Monllau, Josep M.; Borrell, Víctor; Lerin, Carles; de la Iglesia, Núria

    2015-01-01

    Summary Neural stem cells (NSCs) reside in a hypoxic microenvironment within the brain. However, the crucial transcription factors (TFs) that regulate NSC biology under physiologic hypoxia are poorly understood. Here we have performed gene set enrichment analysis (GSEA) of microarray datasets from hypoxic versus normoxic NSCs with the aim of identifying pathways and TFs that are activated under oxygen concentrations mimicking normal brain tissue microenvironment. Integration of TF target (TFT) and pathway enrichment analysis identified the calcium-regulated TF NFATc4 as a major candidate to regulate hypoxic NSC functions. Nfatc4 expression was coordinately upregulated by top hypoxia-activated TFs, while NFATc4 target genes were enriched in hypoxic NSCs. Loss-of-function analyses further revealed that the calcineurin-NFATc4 signaling axis acts as a major regulator of NSC self-renewal and proliferation in vitro and in vivo by promoting the expression of TFs, including Id2, that contribute to the maintenance of the NSC state. PMID:26235896

  11. Transcriptional Profiling of Hypoxic Neural Stem Cells Identifies Calcineurin-NFATc4 Signaling as a Major Regulator of Neural Stem Cell Biology

    Directory of Open Access Journals (Sweden)

    Marta Moreno

    2015-08-01

    Full Text Available Neural stem cells (NSCs reside in a hypoxic microenvironment within the brain. However, the crucial transcription factors (TFs that regulate NSC biology under physiologic hypoxia are poorly understood. Here we have performed gene set enrichment analysis (GSEA of microarray datasets from hypoxic versus normoxic NSCs with the aim of identifying pathways and TFs that are activated under oxygen concentrations mimicking normal brain tissue microenvironment. Integration of TF target (TFT and pathway enrichment analysis identified the calcium-regulated TF NFATc4 as a major candidate to regulate hypoxic NSC functions. Nfatc4 expression was coordinately upregulated by top hypoxia-activated TFs, while NFATc4 target genes were enriched in hypoxic NSCs. Loss-of-function analyses further revealed that the calcineurin-NFATc4 signaling axis acts as a major regulator of NSC self-renewal and proliferation in vitro and in vivo by promoting the expression of TFs, including Id2, that contribute to the maintenance of the NSC state.

  12. Sugar and auxin signaling pathways respond to high-temperature stress during anther development as revealed by transcript profiling analysis in cotton.

    Science.gov (United States)

    Min, Ling; Li, Yaoyao; Hu, Qin; Zhu, Longfu; Gao, Wenhui; Wu, Yuanlong; Ding, Yuanhao; Liu, Shiming; Yang, Xiyan; Zhang, Xianlong

    2014-03-01

    Male reproduction in flowering plants is highly sensitive to high temperature (HT). To investigate molecular mechanisms of the response of cotton (Gossypium hirsutum) anthers to HT, a relatively complete comparative transcriptome analysis was performed during anther development of cotton lines 84021 and H05 under normal temperature and HT conditions. In total, 4,599 differentially expressed genes were screened; the differentially expressed genes were mainly related to epigenetic modifications, carbohydrate metabolism, and plant hormone signaling. Detailed studies showed that the deficiency in S-adenosyl-L-homocysteine hydrolase1 and the inhibition of methyltransferases contributed to genome-wide hypomethylation in H05, and the increased expression of histone constitution genes contributed to DNA stability in 84021. Furthermore, HT induced the expression of casein kinasei (GhCKI) in H05, coupled with the suppression of starch synthase activity, decreases in glucose level during anther development, and increases in indole-3-acetic acid (IAA) level in late-stage anthers. The same changes also were observed in Arabidopsis (Arabidopsis thaliana) GhCKI overexpression lines. These results suggest that GhCKI, sugar, and auxin may be key regulators of the anther response to HT stress. Moreover, phytochrome-interacting factor genes (PIFs), which are involved in linking sugar and auxin and are regulated by sugar, might positively regulate IAA biosynthesis in the cotton anther response to HT. Additionally, exogenous IAA application revealed that high background IAA may be a disadvantage for late-stage cotton anthers during HT stress. Overall, the linking of HT, sugar, PIFs, and IAA, together with our previously reported data on GhCKI, may provide dynamic coordination of plant anther responses to HT stress.

  13. UV-B irradiation changes specifically the secondary metabolite profile in broccoli sprouts: induced signaling overlaps with defense response to biotic stressors.

    Science.gov (United States)

    Mewis, Inga; Schreiner, Monika; Nguyen, Chau Nhi; Krumbein, Angelika; Ulrichs, Christian; Lohse, Marc; Zrenner, Rita

    2012-09-01

    Only a few environmental factors have such a pronounced effect on plant growth and development as ultraviolet light (UV). Concerns have arisen due to increased UV-B radiation reaching the Earth's surface as a result of stratospheric ozone depletion. Ecologically relevant low to moderate UV-B doses (0.3-1 kJ m(-2) d(-1)) were applied to sprouts of the important vegetable crop Brassica oleracea var. italica (broccoli), and eco-physiological responses such as accumulation of non-volatile secondary metabolites were related to transcriptional responses with Agilent One-Color Gene Expression Microarray analysis using the 2×204 k format Brassica microarray. UV-B radiation effects have usually been linked to increases in phenolic compounds. As expected, the flavonoids kaempferol and quercetin accumulated in broccoli sprouts (the aerial part of the seedlings) 24 h after UV-B treatment. A new finding is the specific UV-B-mediated induction of glucosinolates (GS), especially of 4-methylsulfinylbutyl GS and 4-methoxy-indol-3-ylmethyl GS, while carotenoids and Chl levels remained unaffected. Accumulation of defensive GS metabolites was accompanied by increased expression of genes associated with salicylate and jasmonic acid signaling defense pathways and up-regulation of genes responsive to fungal and bacterial pathogens. Concomitantly, plant pre-exposure to moderate UV-B doses had negative effects on the performance of the caterpillar Pieris brassicae (L.) and on the population growth of the aphid Myzus persicae (Sulzer). Moreover, insect-specific induction of GS in broccoli sprouts was affected by UV-B pre-treatment.

  14. Lipid Profile

    Science.gov (United States)

    ... AACC products and services. Advertising & Sponsorship: Policy | Opportunities Lipid Profile Share this page: Was this page helpful? Also ... as: Lipid Panel; Coronary Risk Panel Formal name: Lipid Profile Related tests: Cholesterol ; HDL Cholesterol ; LDL Cholesterol ; Triglycerides ; ...

  15. 大时宽带宽积雷达空间目标距离像估计%Range Profile Estimation of Space Target for Signals with Large Time-Bandwidth Product

    Institute of Scientific and Technical Information of China (English)

    杨利民

    2014-01-01

    A technique based on quadratic phase filters is proposed to obtain the radial velocity and accel-eration of the space target using signals with large time-bandwidth product.The range profile can be got after the corresponding motion compensation using the estimated parameters.First,the echo model is deduced for the targets,and the mathematical expression is yielded after the pulse compression processing to attain the requirement which is not suitable for the normal pulse compression.Then,this algorithm is employed to esti-mate the radial velocity and acceleration of the space target.In order to yield the target’s range profile,the quadratic-cube phase coefficient and range-Doppler coupling are compensated with the estimated parameters. The simulation results demonstrate the feasibility of refining the space target’s range profile.%采用二次相位滤波的方法,估计大时宽带宽积信号下空间目标的速度及加速度,并进行相应的运动补偿后获得目标一维距离像。首先,推导出具有大时宽带宽积信号的空间目标回波模型,分析了利用传统脉压处理后的数学表达式,给出不适合传统脉压处理的条件。为此,利用该方法得出速度和加速度估计值并进行二次和三次相位项系数及距离多普勒耦合的补偿,以获得目标距离像。仿真实验验证了该条件下提取空间目标一维距离像的可行性。

  16. Data Profiling

    OpenAIRE

    Hladíková, Radka

    2010-01-01

    Title: Data Profiling Author: Radka Hladíková Department: Department of Software Engineering Supervisor: Ing. Vladimír Kyjonka Supervisor's e-mail address: Abstract: This thesis puts mind on problems with data quality and data profiling. This Work analyses and summarizes problems of data quality, data defects, process of data quality, data quality assessment and data profiling. The main topic is data profiling as a process of researching data available in existing...

  17. Global gene expression profiling of a mouse model of ovarian clear cell carcinoma caused by ARID1A and PIK3CA mutations implicates a role for inflammatory cytokine signaling

    Directory of Open Access Journals (Sweden)

    Ronald L. Chandler

    2015-09-01

    Full Text Available Ovarian clear-cell carcinoma (OCCC is an aggressive form of epithelial ovarian cancer (EOC. OCCC represents 5–25% of all EOC incidences and is the second leading cause of death from ovarian cancer (Glasspool and McNeish, 2013 [1]. A recent publication by Chandler et al. reported the first mouse model of OCCC that resembles human OCCC both genetically and histologically by inducing a localized deletion of ARID1A and the expression of the PIK3CAH1047R substitution mutation (Chandler et al., 2015 [2]. We utilized Affymetrix Mouse Gene 2.1 ST arrays for the global gene expression profiling of mouse primary OCCC tumor samples and animal-matched normal ovaries to identify cancer-dependent gene expression. We describe the approach used to generate the differentially expressed genes from the publicly available data deposited at the Gene Expression Omnibus (GEO database under the accession number GSE57380. These data were used in cross-species comparisons to publically available human OCCC gene expression data and allowed the identification of coordinately regulated genes in both mouse and human OCCC and supportive of a role for inflammatory cytokine signaling in OCCC pathogenesis (Chandler et al., 2015 [2].

  18. Karolinske psychodynamic profile (KAPP)

    DEFF Research Database (Denmark)

    Mathiesen, Birgit Bork; Søgaard, Ulf

    2006-01-01

    psykologiske testmetoder, assesment, Karolinska psychodynamic profile (KAPP), psykodynamisk profil......psykologiske testmetoder, assesment, Karolinska psychodynamic profile (KAPP), psykodynamisk profil...

  19. The Exoplanet Simple Orbit Fitting Toolbox (ExoSOFT): An Open-source Tool for Efficient Fitting of Astrometric and Radial Velocity Data

    Science.gov (United States)

    Mede, Kyle; Brandt, Timothy D.

    2017-03-01

    We present the Exoplanet Simple Orbit Fitting Toolbox (ExoSOFT), a new, open-source suite to fit the orbital elements of planetary or stellar-mass companions to any combination of radial velocity and astrometric data. To explore the parameter space of Keplerian models, ExoSOFT may be operated with its own multistage sampling approach or interfaced with third-party tools such as emcee. In addition, ExoSOFT is packaged with a collection of post-processing tools to analyze and summarize the results. Although only a few systems have been observed with both radial velocity and direct imaging techniques, this number will increase, thanks to upcoming spacecraft and ground-based surveys. Providing both forms of data enables simultaneous fitting that can help break degeneracies in the orbital elements that arise when only one data type is available. The dynamical mass estimates this approach can produce are important when investigating the formation mechanisms and subsequent evolution of substellar companions. ExoSOFT was verified through fitting to artificial data and was implemented using the Python and Cython programming languages; it is available for public download at https://github.com/kylemede/ExoSOFT under GNU General Public License v3.

  20. AN ASTROMETRIC SEARCH FOR A SUB-STELLAR COMPANION OF THE M8.5 DWARF TVLM 513–46546 USING VERY LONG BASELINE INTERFEROMETRY

    Energy Technology Data Exchange (ETDEWEB)

    Forbrich, Jan; Berger, Edo; Reid, Mark J. [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States)

    2013-11-01

    We conducted multi-epoch very long baseline interferometry observations to search for astrometric reflex motion that would be caused by a sub-stellar companion of the M8.5 dwarf TVLM 513–46546. The observations yield an absolute parallax corresponding to a distance of 10.762 ± 0.027 pc and a proper motion of 78.09 ± 0.17 mas yr{sup –1}. The averaged flux density per epoch varies by a factor of at least three. From the absence of significant residual motion, we place an upper limit on any reflex motion caused by a companion, extending the parameter space covered by previous near-infrared direct-imaging searches. The data exclude a phase space of companion masses and orbital periods ranging from 3.8 M{sub Jup} with an orbital radius of ∼0.05 AU (and an orbital period of 16 days) to 0.3 M{sub Jup} with an orbital radius of ∼0.7 AU (and an orbital period of 710 days)

  1. Signal Words

    Science.gov (United States)

    SIGNAL WORDS TOPIC FACT SHEET NPIC fact sheets are designed to answer questions that are commonly asked by the ... making decisions about pesticide use. What are Signal Words? Signal words are found on pesticide product labels, ...

  2. Signal transduction by growth factor receptors: signaling in an instant

    DEFF Research Database (Denmark)

    Dengjel, Joern; Akimov, Vyacheslav; Blagoev, Blagoy;

    2007-01-01

    -out by mass spectrometry-based proteomics has allowed exciting views on the very early events in signal transduction. Activation profiles of regulated phosphorylation sites on epidermal growth factor receptor and downstream signal transducers showed different kinetics within the first ten seconds...

  3. Leadership Profiles.

    Science.gov (United States)

    Morgan, Robert M.; And Others

    1992-01-01

    Presents profiles of three leaders in the field of educational media and technology: Robert Mills Gagne, Florida State University; Robert Heinich, Indiana University; and Charles Francis Schuller, University of Georgia. (SLW)

  4. Profiling cancer

    DEFF Research Database (Denmark)

    Ciro, Marco; Bracken, Adrian P; Helin, Kristian

    2003-01-01

    In the past couple of years, several very exciting studies have demonstrated the enormous power of gene-expression profiling for cancer classification and prediction of patient survival. In addition to promising a more accurate classification of cancer and therefore better treatment of patients......, gene-expression profiling can result in the identification of novel potential targets for cancer therapy and a better understanding of the molecular mechanisms leading to cancer....

  5. Profiling cancer

    DEFF Research Database (Denmark)

    Ciro, Marco; Bracken, Adrian P; Helin, Kristian

    2003-01-01

    In the past couple of years, several very exciting studies have demonstrated the enormous power of gene-expression profiling for cancer classification and prediction of patient survival. In addition to promising a more accurate classification of cancer and therefore better treatment of patients......, gene-expression profiling can result in the identification of novel potential targets for cancer therapy and a better understanding of the molecular mechanisms leading to cancer....

  6. Analogue Simulation and Orbital Solving Algorithm of Astrometric Exoplanet Detection%基于天体测量法探测系外行星的仿真模拟与轨道求解算法∗

    Institute of Scientific and Technical Information of China (English)

    黄平辉; 季江徽

    2016-01-01

    天体测量法是探测系外行星一种有效的手段,具有其他探测方法所不具备的独特优势,如可以探测到行星的3维轨道参数及确定行星的质量等,这些将为现有的行星样本提供重要补充。高精度的空间天体测量卫星Gaia (Global Astrometric Interferometer for Astrophysics)在2013年成功发射升空,可以预期未来基于其探测数据,将可能发现大量的长周期类木行星。针对α Centauri A、HD 62509和GJ 876系统,根据Gaia单次天体测量精度生成对应的仿真数据,使用Lomb-Scargle周期谱分析行星轨道周期信号,并采用Markov Chain Monte Carlo (MCMC)算法对行星系统进行轨道反演,得到的结果与行星的初始参数相吻合。%Astrometry is an effective method to detect exoplanets. It has many ad-vantages that other detection methods do not bear, such as providing three dimensional planetary orbit and determining the planetary mass. Astrometry will enrich the sample of exoplanets. As the high-precision astrometric satellite Gaia (Global Astrometry in-terferometer for Astrophysics) was launched in 2013, there will be abundant long-period Jupiter-size planets to be discovered by Gaia. In this paper, we specify theαCentauri A, HD 62509, and GJ 876 systems, and generate the synthetic astrometric data with the single astrometric precision of Gaia. Then we use the Lomb-Scargle periodogram to analyse the signature of planets and the Markov Chain Monte Carlo (MCMC) al-gorithm to fit the orbit of planets. The simulation results are well coincide with the initial solutions.

  7. ATP signals

    DEFF Research Database (Denmark)

    Novak, Ivana

    2016-01-01

    The Department of Biology at the University of Copenhagen explains the function of ATP signalling in the pancreas......The Department of Biology at the University of Copenhagen explains the function of ATP signalling in the pancreas...

  8. Profile summary.

    Science.gov (United States)

    2003-01-01

    All drugs appearing in the Adis Profile Summary table have been selected based on information contained in R&D Insight trade mark, a proprietary product of Adis International. The information in the profiles is gathered from the world's medical and scientific literature, at international conferences and symposia, and directly from the developing companies themselves. The emphasis of Drugs in R&D is on the clinical potential of new drugs, and selection of agents for inclusion is based on products in late-phase clinical development that have recently had a significant change in status.

  9. Astrometrically Registered Simultaneous Observations of the 22 GHz H$_2$O and the 43GHz SiO masers towards R Leonis Minoris using KVN and Source/Frequency Phase Referencing

    CERN Document Server

    Dodson, Richard; Jung, Tae-Hyun; Sohn, Bong-Won; Byun, Do-Young; Cho, Se-Hyung; Lee, Sang-Sung; Kim, Jongsoo; Kim, Kee-Tae; Oh, Chung-Sik; Han, Seog-Tae; Je, Do-Heung; Chung, Moon-Hee; Wi, Seog-Oh; Kang, Jiman; Lee, Jung-Won; Chung, Hyunsoo; Kim, Hyo-Ryoung; Kim, Hyun-Goo; Lee, Chang-Hoon; Roh, Duk-Gyoo; Oh, Se-Jin; Yeom, Jae-Hwan; Song, Min-Gyu; Kang, Yong-Woo

    2014-01-01

    Oxygen-rich Asymptotic Giant Branch (AGB) stars can be intense emitters of SiO ($v$=1 and 2, J=1$\\rightarrow$0) and H$_2$O maser lines at 43 and 22 GHz, respectively. VLBI observations of the maser emission provide a unique tool to probe the innermost layers of the circumstellar envelopes in AGB stars. Nevertheless, the difficulties in achieving astrometrically aligned \\water\\ and $v$=1 and $v$=2 SiO maser maps have traditionally limited the physical constraints that can be placed on the SiO maser pumping mechanism. We present phase referenced simultaneous spectral-line VLBI images for the SiO $v$=1 and $v$=2, J=1$\\rightarrow$0, and H$_2$O maser emission around the AGB star R\\,LMi, obtained from the Korean VLBI Network (KVN). The simultaneous multi-channel receivers of the KVN offer great possibilities for astrometry in the frequency domain. With this facility we have produced images with bona-fide absolute astrometric registration between high frequency maser transitions of different species to provide the p...

  10. ASTERISMS IDENTIFICATION USING ASTROMETRIC DATA

    Directory of Open Access Journals (Sweden)

    R. B. Orellana

    2008-01-01

    Full Text Available Se estudian las regiones de los objetos Collinder 399, Upgren 1 y Melotte 227 clasi cados como asterismos en la literatura reciente. Se utilizan las posiciones y movimientos propios obtenidos del cat logo astrom trico UCAC2 y de su suplemento de estrellas brillantes, y se aplica el m todo de Vasilevskis-Sanders modi cado porla distribuci n espacial para con rmar la naturaleza de estos grupos de estrellas.

  11. STEPS: JPL's Astrometric Exoplanet Survey

    Science.gov (United States)

    Shaklan, Stuart; Pravdo, Steve

    2008-01-01

    Presentation topics include: STEPS ground-based astrometry at Hale Telescope; the instrument; why astronomy and why M-dwarfs; motion of center of light about center of mass in photocentric orbit; photocentric motion vs. fractional mass; high-resolution imaging of STEPS targets; GU 802 p one possible orbit plotted with data, Keplerian frame; GJ 802 results; STEPS future; and a bibliography of STEPS papers.

  12. The Unification of Astrometric Catalogues

    Science.gov (United States)

    Stock, J.; Abad, C.

    1988-06-01

    RESUMEN Se desarrolla un metodo de interpolaci6n para detectar diferencias sistematicas entre catalogos de posiciones y movimientos propios y se aplica a los catalogos AGK3 y Santiago 67, usando los cata'logos No. 1 y No. 2 del Cfrculo Meridiano Carlsberg como sistema de referencia. ABSTRACT An interpolation mechanism is developed for the detection of systematic differences between position and proper motion catalogues and is applied to the AGK3 and Santiago 67 catalogues, using the Carlsberg Meridian Circle catalogues No. 1 and No. 2 as reference sources. Key words: ASTROMETRY

  13. Signaling aggression.

    Science.gov (United States)

    van Staaden, Moira J; Searcy, William A; Hanlon, Roger T

    2011-01-01

    From psychological and sociological standpoints, aggression is regarded as intentional behavior aimed at inflicting pain and manifested by hostility and attacking behaviors. In contrast, biologists define aggression as behavior associated with attack or escalation toward attack, omitting any stipulation about intentions and goals. Certain animal signals are strongly associated with escalation toward attack and have the same function as physical attack in intimidating opponents and winning contests, and ethologists therefore consider them an integral part of aggressive behavior. Aggressive signals have been molded by evolution to make them ever more effective in mediating interactions between the contestants. Early theoretical analyses of aggressive signaling suggested that signals could never be honest about fighting ability or aggressive intentions because weak individuals would exaggerate such signals whenever they were effective in influencing the behavior of opponents. More recent game theory models, however, demonstrate that given the right costs and constraints, aggressive signals are both reliable about strength and intentions and effective in influencing contest outcomes. Here, we review the role of signaling in lieu of physical violence, considering threat displays from an ethological perspective as an adaptive outcome of evolutionary selection pressures. Fighting prowess is conveyed by performance signals whose production is constrained by physical ability and thus limited to just some individuals, whereas aggressive intent is encoded in strategic signals that all signalers are able to produce. We illustrate recent advances in the study of aggressive signaling with case studies of charismatic taxa that employ a range of sensory modalities, viz. visual and chemical signaling in cephalopod behavior, and indicators of aggressive intent in the territorial calls of songbirds.

  14. 先天性巨结肠中HA117及SHH信号通路的表达及意义%Expression profiles and significance of HA117 and SHH signaling pathway in Hirschsprung's disease

    Institute of Scientific and Technical Information of China (English)

    滕银平; 金先庆; 李力; 吴欢; 王鑫; 罗媛圆

    2016-01-01

    目的 研究HA117及SHH信号通路(SHH、Ptch1、Gli1)在先天性巨结肠(Hirschsprung's disease,HD)中的表达情况,以探讨其与HD发生发展中的关系.方法 收集重庆医科大学附属儿童医院2014年至2015年经手术确诊为HD患儿的巨结肠组织标本30例,肠段分吻合段(正常对照组)、扩张段和痉挛段(实验组),运用实时荧光定量PCR (quantitative real-time PCR,qRT-PCR)、蛋白质印迹(Western blot)和免疫组织化学方法检测HA117、SHH信号途径在各段中的表达情况,对其进行定量和定性并比较.结果 qRT-PCR示痉挛段HA117mRNA表达水平为1.52±0.17,明显高于吻合段的0.28±0.04,差异具有统计学意义(P<0.05).痉挛段SHH、Ptch1、Gli1mRNA表达水平分别为0.53±0.11、0.48±0.11、0.62±0.13,明显低于吻合段(1.23±0.26、1.20±0.22、1.71±0.46),差异具有统计学意义(P<0.05);Western-Blot示SHH、Ptch1、Gli1蛋白相对表达水平与mRNA表达水平一致,差异均具有统计学意义(P<0.05);免疫组织化学(DAB染色)结果提示:痉挛段SHH、Ptch1、Gli1染色明显弱于吻合段.结论 HA117及SHH信号通路可能与肠神经系统(ENS)发育有着密切的联系,这可能是HD病理发生的重要作用机制之一.%Objective To explore the relationship between HA117 and SHH signaling pathway (SHH,Ptch1 & Gli1) and Hirschsprung's disease (HD) by analyzing their expression profiles in different colonic segments of HD patients.Methods Colonic specimens were collected intraoperatively from 30 patients diagnosed with HD at our hospital from 2014 to 2015.Proximal anastomosis,dilated segment and stenotic segment were designated.The mRNA levels of HA117,SHH,Smo,Ptch1 and Gli1 were evaluated by quantitative real-time polymerase chain reaction (PCR).And the protein levels of SHH,Ptch1 and Gli1 were examined by Western blot and immunohistochemistry.Results The mRNA levels of HA117 were significantly higher in stenotic segment than those in proximal

  15. The late-type stellar density profile in the Galactic Center: A statistical approach

    CERN Document Server

    Chappell, Samantha N; Do, Tuan; Martinez, Gregory D; Yelda, Sylvana; Sitarski, Breann N; Lu, Jessica R; Morris, Mark R

    2016-01-01

    The late-type stellar population in the Galactic Center was first predicted to reside in a dynamically relaxed cusp (power law slope ranging from 3/2 to 7/4). However, observations - which rely on models to correct for projection effects - have suggested a flat distribution instead. The need for this correction is due to the lack of information regarding the line-of-sight distances. With a two decade long baseline in astrometric measurements, we are now able to measure significant projected radial accelerations, six of which are newly reported here, that directly constrain line-of-sight distances. Here we present a statistical approach to take advantage of this information and more accurately constrain the shape of the radial density profile of the late-type stellar population in the Galactic Center.

  16. miR-155, identified as anti-metastatic by global miRNA profiling of a metastasis model, inhibits cancer cell extravasation and colonization in vivo and causes significant signaling alterations

    DEFF Research Database (Denmark)

    Gravgaard, Karina Hedelund; Terp, Mikkel G; Lund, Rikke R

    2015-01-01

    To gain insight into miRNA regulation in metastasis formation, we used a metastasis cell line model that allows investigation of extravasation and colonization of circulating cancer cells to lungs in mice. Using global miRNA profiling, 28 miRNAs were found to exhibit significantly altered express...

  17. Current Progress on Second Astrometric Satellite (Gaia) Project%第二个天体测量卫星(Gaia)项目的进展

    Institute of Scientific and Technical Information of China (English)

    金文敬

    2011-01-01

    简述了第二个天体测量卫星Gaia(将于2013年3月发射)项目的科学意义,并给出了该项目的组织工作和最近的进展.描述了Gala观测资料处理的基本原理,以及与依巴谷观测资料处理的不同点.介绍了Gaia参考架构建的考虑,以及为了构建微角秒量级的参考架,应在自行中加入系统差改正,如长期光行差、引力波效应、宇宙膨胀各向异性的影响,弱的微引力透镜和微引力透镜噪声效应等.介绍了Gaia光学参考架与射电参考架ICRFL2之间建立联系过程中,选择河外射电源的准则,其中包括源的核漂移和光学长期变化监测等.最后,提出了我国现有设备参与支持Gaia的地基观测,以及正在研制的65 m射电天线在射电天体测量方面可以开展的若干课题.%The scientific goals of the second astrometric satellite (Gaia) are briefly described.Gaia will be launched in March 2013. The current progress of this project is presented in this paper, especially the basic principle of observational data processing method is given. The difference between Gaia and Hipparcos data processing is also pointed out. In order to attain the accuracy with micro-arcsecond in proper motion some systematic errors such as secular aberration drift, effects of the low-frequency gravitational wave, the Universe expansion anisotropy, the weak microlensing and the microlensing noise should be considered. The construction of Gaia optical reference frame, including the selection of quasars and the attainable accuracy, is mentioned. As for the link between Gaia reference and the radio reference frame ICRF-2, which is adopted by IAU at the 27 th IAU general assembly, the core drift and detection of luminosity in long-term as accident errors should be considered. Finally, through the collaboration with some countries some research subjects, which can be carried out on ground based observations with current instruments and 65 m radio telescope to be

  18. MPI Profiling

    Energy Technology Data Exchange (ETDEWEB)

    Han, D K; Jones, T R

    2005-02-11

    The Message Passing Interface (MPI) is the de facto message-passing standard for massively parallel programs. It is often the case that application performance is a crucial factor, especially for solving grand challenge problems. While there have been many studies on the scalability of applications, there have not been many focusing on the specific types of MPI calls being made and their impact on application performance. Using a profiling tool called mpiP, a large spectrum of parallel scientific applications were surveyed and their performance results analyzed.

  19. Quantum profiles

    CERN Document Server

    Bernstein, Jeremy

    1991-01-01

    For the prominent science writer Jeremy Bernstein, the profile is the most congenial way of communicating science. Here, in what he labels a "series of conversations carried on in the reader's behalf and my own," he evokes the tremendous intellectual excitement of the world of modern physics, especially the quantum revolution. Drawing on his well-known talent for explaining the most complex scientific ideas for the layperson, Bernstein gives us a lively sense of what the issues of quantum mechanics are and of various ways in which individual physicists approached them.The author begins this se

  20. Bayesian signaling

    OpenAIRE

    Hedlund, Jonas

    2014-01-01

    This paper introduces private sender information into a sender-receiver game of Bayesian persuasion with monotonic sender preferences. I derive properties of increasing differences related to the precision of signals and use these to fully characterize the set of equilibria robust to the intuitive criterion. In particular, all such equilibria are either separating, i.e., the sender's choice of signal reveals his private information to the receiver, or fully disclosing, i.e., the outcome of th...

  1. Comparison of binning approaches for pulsed photothermal temperature profiling

    Science.gov (United States)

    Milanič, Matija; Majaron, Boris

    2009-07-01

    In experiments and numerical simulations of pulsed photothermal temperature profiling, we compare three signal binning approaches. In uniform binning n subsequent signal data points are averaged, quadratic binning follows from the characteristic of thermal diffusion, and geometrical binning utilizes geometric progression. Our experiment was performed on collagen gel samples with absorbing layers located at various subsurface depths. From measured PPTR signals laser-induced temperature profiles were reconstructed using spectrally composite kernel. The simulated PPTR signals of temperature profiles resembling experimental temperature profiles contain noise with characteristics consistent with our experimental system. In addition, we simulated PPTR signal of a biopsy-defined port-wine stain skin geometry. In PPTR temperature profiling of collagen gel samples, quadratic binning results in optimal reconstructions for shallow absorbing structures, while uniform binning performs optimally for deeper absorbing structures. Overall, geometric binning yields least accurate reconstructions, especially for deeper absorbing layers.

  2. The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein

    Directory of Open Access Journals (Sweden)

    Claudia C. Márquez-Mota

    2016-09-01

    Full Text Available Between 6% and 11% of the world’s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1-signaling pathway in: (1 healthy; and (2 protein restricted rats. (1 In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C, soy (S, black bean (B, B + Corn (BCr, Pea (P, spirulina (Sp, sesame (Se or Corn (Cr. Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2 In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

  3. The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein.

    Science.gov (United States)

    Márquez-Mota, Claudia C; Rodriguez-Gaytan, Cinthya; Adjibade, Pauline; Mazroui, Rachid; Gálvez, Amanda; Granados, Omar; Tovar, Armando R; Torres, Nimbe

    2016-09-20

    Between 6% and 11% of the world's population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

  4. Cherenkov light-based beam profiling for ultrarelativistic electron beams

    Energy Technology Data Exchange (ETDEWEB)

    Adli, E., E-mail: Erik.Adli@fys.uio.no [Department of Physics, University of Oslo, N-0316 Oslo (Norway); SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025 (United States); Gessner, S.J.; Corde, S.; Hogan, M.J. [SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025 (United States); Bjerke, H.H. [SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025 (United States); Department of Physics, Norwegian University of Science and Technology, N-7491 Trondheim (Norway)

    2015-05-21

    We describe a beam profile monitor design based on Cherenkov light emitted from a charged particle beam in an air gap. The main components of the profile monitor are silicon wafers used to reflect Cherenkov light onto a camera lens system. The design allows for measuring large beam sizes, with large photon yield per beam charge and excellent signal linearity with beam charge. The profile monitor signal is independent of the particle energy for ultrarelativistic particles. Different design and parameter considerations are discussed. A Cherenkov light-based profile monitor has been installed at the FACET User Facility at SLAC. We report on the measured performance of this profile monitor.

  5. Developmental expression profiles of axon guidance signaling and the immune system in the marmoset cortex: potential molecular mechanisms of pruning of dendritic spines during primate synapse formation in late infancy and prepuberty (I).

    Science.gov (United States)

    Sasaki, Tetsuya; Oga, Tomofumi; Nakagaki, Keiko; Sakai, Kazuhisa; Sumida, Kayo; Hoshino, Kohei; Miyawaki, Izuru; Saito, Koichi; Suto, Fumikazu; Ichinohe, Noritaka

    2014-02-14

    The synapse number and the related dendritic spine number in the cerebral cortex of primates shows a rapid increase after birth. Depending on the brain region and species, the number of synapses reaches a peak before adulthood, and pruning takes place after this peak (overshoot-type synaptic formation). Human mental disorders, such as autism and schizophrenia, are hypothesized to be a result of either too weak or excessive pruning after the peak is reached. Thus, it is important to study the molecular mechanisms underlying overshoot-type synaptic formation, particularly the pruning phase. To examine the molecular mechanisms, we used common marmosets (Callithrix jacchus). Microarray analysis of the marmoset cortex was performed in the ventrolateral prefrontal, inferior temporal, and primary visual cortices, where changes in the number of dendritic spines have been observed. The spine number of all the brain regions above showed a peak at 3 months (3 M) after birth and gradually decreased (e.g., at 6 M and in adults). In this study, we focused on genes that showed differential expression between ages of 3 M and 6 M and on the differences whose fold change (FC) was greater than 1.2. The selected genes were subjected to canonical pathway analysis, and in this study, we describe axon guidance signaling, which had high plausibility. The results showed a large number of genes belonging to subsystems within the axon guidance signaling pathway, macrophages/immune system, glutamate system, and others. We divided the data and discussion of these results into 2 papers, and this is the first paper, which deals with the axon guidance signaling and macrophage/immune system. Other systems will be described in the next paper. Many components of subsystems within the axon guidance signaling underwent changes in gene expression from 3 M to 6 M so that the synapse/dendritic spine number would decrease at 6 M. Thus, axon guidance signaling probably contributes to the decrease in

  6. Changes of release IFN-γmRNAs expression profiling and signaling pathways of ESAT-6 stimulated CD4+ T lymphocytes from tuberculosis patients%ESAT-6刺激结核患者CD4+ T淋巴细胞前后释放IFN-γmRNA差异表达及信号通路变化

    Institute of Scientific and Technical Information of China (English)

    张焰; 王晓玮; 程筱雯; 徐元宏

    2016-01-01

    目的:利用全基因组表达谱芯片对早期分泌靶向抗原6(ESAT-6)刺激结核患者外周血CD4+ T淋巴细胞前后释放γ-干扰素( IFN-γ) mRNA进行检测,并对mRNA在刺激过程中可能涉及到的信号通路变化进行分析。方法抽取3例初诊结核患者外周静脉血各10 ml,密度梯度离心法分离获得外周血单个核细胞( PBMC), ESAT-6刺激PBMC,6 h后磁珠法分选CD4+ T淋巴细胞,提取细胞总RNA,全基因组表达谱芯片检测刺激前后差异表达基因。利用GO分析对差异表达的基因进行功能分类, KEGG 进行信号通路分析。结果 ESAT-6刺激后差异表达的mRNA共有2297条,相对高表达的 mRNA 有1071条,相对低表达的 mRNA 有1226条(>2倍变化且P2 fold changes and P <0. 05 ) . 17 signaling pathways were involved based on mRNAs expression data and 5 of which were involved in tuberculosis IFN-γ re-lease( JAK-STAT signaling pathway, toll like receptor signaling pathway, NF-κB signaling pathway, MAPK signa-ling pathway, and PI3K-Akt signaling pathway). Conclusion After ESAT-6 stimulation, obvious changes of mR-NAs expression profiling are observed. Those differentially expressed mRNAs play important roles in regulating sig-naling pathways in CD4 + T lymphocytes tuberculosis IFN-γ release.

  7. Comparison of the gene expression profiles of human fetal cortical astrocytes with pluripotent stem cell derived neural stem cells identifies human astrocyte markers and signaling pathways and transcription factors active in human astrocytes.

    Science.gov (United States)

    Malik, Nasir; Wang, Xiantao; Shah, Sonia; Efthymiou, Anastasia G; Yan, Bin; Heman-Ackah, Sabrina; Zhan, Ming; Rao, Mahendra

    2014-01-01

    Astrocytes are the most abundant cell type in the central nervous system (CNS) and have a multitude of functions that include maintenance of CNS homeostasis, trophic support of neurons, detoxification, and immune surveillance. It has only recently been appreciated that astrocyte dysfunction is a primary cause of many neurological disorders. Despite their importance in disease very little is known about global gene expression for human astrocytes. We have performed a microarray expression analysis of human fetal astrocytes to identify genes and signaling pathways that are important for astrocyte development and maintenance. Our analysis confirmed that the fetal astrocytes express high levels of the core astrocyte marker GFAP and the transcription factors from the NFI family which have been shown to play important roles in astrocyte development. A group of novel markers were identified that distinguish fetal astrocytes from pluripotent stem cell-derived neural stem cells (NSCs) and NSC-derived neurons. As in murine astrocytes, the Notch signaling pathway appears to be particularly important for cell fate decisions between the astrocyte and neuronal lineages in human astrocytes. These findings unveil the repertoire of genes expressed in human astrocytes and serve as a basis for further studies to better understand astrocyte biology, especially as it relates to disease.

  8. Prosocial Signalling

    DEFF Research Database (Denmark)

    Kahsay, Goytom Abraha

    suggested that consumers pay price premium because this sends the signal that the consumer has prosocial preferences and a few empirical studies have documented that reputation plays a key role when consumers choose products containing prosocial components. However, little is known about consumers...... consumer goods and presents empirical evidences from a natural consumption data. This thesis also investigates consumers’ behaviour under a newly introduced pricing system called Pay-What-You-Want (PWYW) and investigates empirically whether reputation signalling can be used as a policy instrument in other...... on the role of social network in facilitating factor input transactions and the role of reputation in reducing enforcement. Finally, the third part consists of one paper which is concerned with investigating the effect of climate change and adaptation policy on agricultural production in Eastern Africa...

  9. COMPENDEX Profiling Guide.

    Science.gov (United States)

    Standera, Oldrich

    This manual provides instructions for completing the COMPENDEX (Computerized Engineering Index) Profile Submission Form used to prepare Current Information Selection (CIS) profiles. An annotated bibliography lists nine items useful in searching for proper profile words. (AB)

  10. Signal Processing

    Science.gov (United States)

    1989-03-01

    34ESPIRIT Estimation of signal parameters via rotational imvariance techin+I,-- 1\\I111;1 Smith. A. Faradani "Local and ( Moba ! tomography" I’ Nitlerer and...Feb 1 - Jul 30 Friedman, Avner IMA Gader, Paul University of Wisconsin Jun 27 - Jul 24 Games , Richard MITRE Corp Jun 27 - Aug 5 Garvan, Francis U. of...Gader, Paul University of Wisconsin Jun 27 - Jul 24 Games , Richard MITRE Corp Jun 27 - Aug 5 Garvan, Francis U. of Wisconsin Jun 26 - Jul 31 Habsieger

  11. Targeting FGFR Signaling in Cancer.

    Science.gov (United States)

    Touat, Mehdi; Ileana, Ecaterina; Postel-Vinay, Sophie; André, Fabrice; Soria, Jean-Charles

    2015-06-15

    The fibroblast growth factor signaling pathway (FGFR signaling) is an evolutionary conserved signaling cascade that regulates several basic biologic processes, including tissue development, angiogenesis, and tissue regeneration. Substantial evidence indicates that aberrant FGFR signaling is involved in the pathogenesis of cancer. Recent developments of deep sequencing technologies have allowed the discovery of frequent molecular alterations in components of FGFR signaling among several solid tumor types. Moreover, compelling preclinical models have demonstrated the oncogenic potential of these aberrations in driving tumor growth, promoting angiogenesis, and conferring resistance mechanisms to anticancer therapies. Recently, the field of FGFR targeting has exponentially progressed thanks to the development of novel agents inhibiting FGFs or FGFRs, which had manageable safety profiles in early-phase trials. Promising treatment efficacy has been observed in different types of malignancies, particularly in tumors harboring aberrant FGFR signaling, thus offering novel therapeutic opportunities in the era of precision medicine. The most exciting challenges now focus on selecting patients who are most likely to benefit from these agents, increasing the efficacy of therapies with the development of novel potent compounds and combination strategies, and overcoming toxicities associated with FGFR inhibitors. After examination of the basic and translational research studies that validated the oncogenic potential of aberrant FGFR signaling, this review focuses on recent data from clinical trials evaluating FGFR targeting therapies and discusses the challenges and perspectives for the development of these agents.

  12. Arcuate nucleus transcriptome profiling identifies ankyrin repeat and suppressor of cytokine signalling box-containing protein 4 as a gene regulated by fasting in central nervous system feeding circuits.

    Science.gov (United States)

    Li, J-Y; Kuick, R; Thompson, R C; Misek, D E; Lai, Y-M; Liu, Y-Q; Chai, B-X; Hanash, S M; Gantz, I

    2005-06-01

    The arcuate nucleus of the hypothalamus is a primary site for sensing blood borne nutrients and hormonal messengers that reflect caloric status. To identify novel energy homeostatic genes, we examined RNA extracts from the microdissected arcuate nucleus of fed and 48-h fasted rats using oligonucleotide microarrays. The relative abundance of 118 mRNA transcripts was increased and 203 mRNA transcripts was decreased during fasting. One of the down-regulated mRNAs was ankyrin-repeat and suppressor of cytokine signalling box-containing protein 4 (Asb-4). The predicted structure of Asb-4 protein suggested that it might encode an intracellular regulatory protein, and therefore its mRNA expression was investigated further. Reverse transcription quantitative polymerase chain reaction was used to validate down-regulation of Asb-4 mRNA in the arcuate nucleus of the fasted Sprague-Dawley rat (relative expression of Asb-4 mRNA: fed = 4.66 +/- 0.26; fasted = 3.96 +/- 0.23; n = 4, P regulation was also demonstrated in the obese fa/fa Zucker rat, another model of energy disequilibrium (relative expression of Asb-4 mRNA: lean Zucker = 3.91 +/- 0.32; fa/fa = 2.93 +/- 0.26; n = 5, P fasted state, the percentage of POMC neurones expressing Asb-4 mRNA drops to 73.2% (P fasted POMC neurone is markedly decreased. Conversely, expression of Asb-4 mRNA by NPY neurones in the fasted state is modestly increased to 52.7% (P < 0.05). Based on its differential expression, neuroanatomical distribution and colocalisation, we hypothesise that Asb-4 is a gene involved in energy homeostasis.

  13. Testing Gravity using Void Profiles

    Science.gov (United States)

    Cai, Yan-Chuan; Padilla, Nelson; Li, Baojiu

    2016-10-01

    We investigate void properties in f(R) models using N-body simulations, focusing on their differences from General Relativity (GR) and their detectability. In the Hu-Sawicki f(R) modified gravity (MG) models, the halo number density profiles of voids are not distinguishable from GR. In contrast, the same f(R) voids are more empty of dark matter, and their profiles are steeper. This can in principle be observed by weak gravitational lensing of voids, for which the combination of a spectroscopic redshift and a lensing photometric redshift survey over the same sky is required. Neglecting the lensing shape noise, the f(R) model parameter amplitudes fR0=10-5 and 10-4 may be distinguished from GR using the lensing tangential shear signal around voids by 4 and 8 σ for a volume of 1 (Gpc/h)3. The line-of-sight projection of large-scale structure is the main systematics that limits the significance of this signal for the near future wide angle and deep lensing surveys. For this reason, it is challenging to distinguish fR0=10-6 from GR. We expect that this can be overcome with larger volume. The halo void abundance being smaller and the steepening of dark matter void profiles in f(R) models are unique features that can be combined to break the degeneracy between fR0 and σ8.

  14. Prosocial Signalling

    DEFF Research Database (Denmark)

    Kahsay, Goytom Abraha

    In contrast to the standard economic theory predictions, it seems clear that people do spend their time and resource to benefit others. Many lab and field experiment studies show that people display prosocial preferences such as altruism, reciprocity and conditional cooperation, fairness, etc...... economic and environmental domains. This thesis consists of five papers which can be divided into three parts. The first part consists of three papers which all investigate consumers’ behavior when prosocial signalling is important. The second part of the thesis consists of one paper which focuses...... empirical evidence on the role of indigenous social networks, namely iddir associations, in facilitating factor-input transactions among smallholder farmers. Iddir networks provide information advantages, trust, and reputation based contract enforcement. It finds that iddir membership improves household...

  15. Florigen signaling.

    Science.gov (United States)

    Tsuji, Hiroyuki; Taoka, Ken-Ichiro

    2014-01-01

    Florigen is a systemic signal that promotes flowering. Its molecular nature is a conserved FLOWERING LOCUS T (FT) protein that belongs to the PEBP family. FT is expressed in the leaf phloem and transported to the shoot apical meristem where it initiates floral transition. In the cells of the meristem, FT binds 14-3-3 proteins and bZIP transcription factor FD to form the florigen activation complex, FAC, which activates floral meristem identity genes such as AP1. The FAC model provides molecular basis for multiple functions of FT beyond flowering through changes of its partners and transcriptional targets. The surface of FT protein includes several regions essential for transport and functions, suggesting the binding of additional components that support its function. FT expression is under photoperiodic control, involving a conserved GIGANTEA-CONSTANS-FT regulatory module with species-specific modifications that contribute variations of flowering time in natural populations.

  16. Optical profiler for low reflectance ultrasmooth surfaces

    Science.gov (United States)

    van Wingerden, Johannes; Frankena, Hans J.; van der Zwan, Bertram A.

    1992-11-01

    Design considerations are discussed for an optical profiler consisting of an interference microscope adapted for phase shifting interferometry. The influence of several errors on the accuracy of the profiler are estimated. Specific attention is paid to the case of low-reflectance surfaces, which have to be measured with extremely high precision (e.g., uncoated bowl-feed polished glass surfaces). The accuracy-limiting factor for the measurement of these low- reflectance ultrasmooth surfaces is shown to be the inaccuracy of the measured intensity. A significant increase in accuracy is obtained by using a mercury arc lamp, which has a very high brightness, yielding a larger intensity signal and thus reducing the signal-to-noise ratio. Extensive tests results of such an optical profiler using a Linnik interference microscope are presented, including the determination of the estimated reference profile accuracy. A measurement accuracy of 0.015 nm rms was obtained for uncoated glass surfaces by averaging 64 profiles. The accuracy of the estimated reference profile using 32 measurements was determined as being about 0.03 nm rms.

  17. DNA profiles from fingermarks.

    Science.gov (United States)

    Templeton, Jennifer E L; Linacre, Adrian

    2014-11-01

    Criminal investigations would be considerably improved if DNA profiles could be routinely generated from single fingermarks. Here we report a direct DNA profiling method that was able to generate interpretable profiles from 71% of 170 fingermarks. The data are based on fingermarks from all 5 digits of 34 individuals. DNA was obtained from the fingermarks using a swab moistened with Triton-X, and the fibers were added directly to one of two commercial DNA profiling kits. All profiles were obtained without increasing the number of amplification cycles; therefore, our method is ideally suited for adoption by the forensic science community. We indicate the use of the technique in a criminal case in which a DNA profile was generated from a fingermark on tape that was wrapped around a drug seizure. Our direct DNA profiling approach is rapid and able to generate profiles from touched items when current forensic practices have little chance of success.

  18. HOPWA Performance Profiles

    Data.gov (United States)

    Department of Housing and Urban Development — HOPWA Performance Profiles are generated quarterly for all agencies receiving HOPWA formula or competitive grants. Performance Profiles are available at the national...

  19. GHGRP Industrial Profiles

    Science.gov (United States)

    EPA's Greenhouse Gas Reporting Program periodically produces detailed profiles of the various industries that report under the program. These profiles contain detailed analyses. This page hosts data highlights for all sectors.

  20. Microwave Radiometer Profiler

    Data.gov (United States)

    Oak Ridge National Laboratory — The microwave radiometer profiler (MWRP) provides vertical profiles of temperature, humidity, and cloud liquid water content as a function of height or pressure at...

  1. SPE-NMR metabolite sub-profiling of urine

    NARCIS (Netherlands)

    Jacobs, D.M.; Spiesser, L.; Garnier, M.; Roo, de N.; Dorsten, van F.; Hollebrands, B.; Velzen, van E.; Draijer, R.; Duynhoven, van J.P.M.

    2012-01-01

    NMR-based metabolite profiling of urine is a fast and reproducible method for detection of numerous metabolites with diverse chemical properties. However, signal overlap in the (1)H NMR profiles of human urine may hamper quantification and identification of metabolites. Therefore, a new method has b

  2. SPE-NMR metabolite sub-profiling of urine

    NARCIS (Netherlands)

    Jacobs, D.M.; Spiesser, L.; Garnier, M.; Roo, de N.; Dorsten, van F.; Hollebrands, B.; Velzen, van E.; Draijer, R.; Duynhoven, van J.P.M.

    2012-01-01

    NMR-based metabolite profiling of urine is a fast and reproducible method for detection of numerous metabolites with diverse chemical properties. However, signal overlap in the (1)H NMR profiles of human urine may hamper quantification and identification of metabolites. Therefore, a new method has

  3. Kir3 channel signaling complexes: Focus on opioid receptor signaling

    Directory of Open Access Journals (Sweden)

    Karim eNagi

    2014-07-01

    Full Text Available Opioids are among the most effective drugs to treat severe pain. They produce their analgesic actions by specifically activating opioid receptors located along the pain perception pathway where they inhibit the flow of nociceptive information. This inhibition is partly accomplished by activation of hyperpolarizing G protein-coupled inwardly-rectifying potassium (GIRK or Kir3 channels. Kir3 channels control cellular excitability in the central nervous system and in the heart and, because of their ubiquitous distribution, they mediate the effects of a large range of hormones and neurotransmitters which, upon activation of corresponding G protein-coupled receptors (GPCRs lead to channel opening. Here we analyze GPCR signaling via these effectors in reference to precoupling and collision models. Existing knowledge on signaling bias is discussed in relation to these models as a means of developing strategies to produce novel opioid analgesics with an improved side effects profile.

  4. One laser pulse generates two photoacoustic signals

    CERN Document Server

    Gao, Fei; Zheng, Yuanjin

    2016-01-01

    Photoacoustic sensing and imaging techniques have been studied widely to explore optical absorption contrast based on nanosecond laser illumination. In this paper, we report a long laser pulse induced dual photoacoustic (LDPA) nonlinear effect, which originates from unsatisfied stress and thermal confinements. Being different from conventional short laser pulse illumination, the proposed method utilizes a long square-profile laser pulse to induce dual photoacoustic signals. Without satisfying the stress confinement, the dual photoacoustic signals are generated following the positive and negative edges of the long laser pulse. More interestingly, the first expansion-induced photoacoustic signal exhibits positive waveform due to the initial sharp rising of temperature. On the contrary, the second contraction-induced photoacoustic signal exhibits exactly negative waveform due to the falling of temperature, as well as pulse-width-dependent, signal amplitude which is caused by the concurrent heat accumulation and ...

  5. Signal Processing of Random Physiological Signals

    CERN Document Server

    Lessard, Charles

    2006-01-01

    Signal Processing of Random Physiological Signals presents the most widely used techniques in signal and system analysis. Specifically, the book is concerned with methods of characterizing signals and systems. Author Charles Lessard provides students and researchers an understanding of the time and frequency domain processes which may be used to evaluate random physiological signals such as brainwave, sleep, respiratory sounds, heart valve sounds, electromyograms, and electro-oculograms.Another aim of the book is to have the students evaluate actual mammalian data without spending most or all

  6. Profiling Metacognition in Gambling Disorder.

    Science.gov (United States)

    Spada, Marcantonio M; Giustina, Lucia; Rolandi, Silvia; Fernie, Bruce A; Caselli, Gabriele

    2015-09-01

    Preliminary research has indicated that general facets of metacognition are associated with problem gambling. In the present study we aimed to investigate whether specific facets of metacognition play a role in explaining gambling initiation and perseveration in individuals presenting with gambling disorder. To investigate: (1) the presence of metacognitive beliefs about gambling; (2) the goal of gambling, and its start and stop signals; and (3) the perceived impact of gambling on self-consciousness. Ten individuals with a diagnosis of gambling disorder were assessed using metacognitive profiling, a semi-structured interview. Findings indicated that all participants endorsed both positive and negative metacognitive beliefs about gambling. The primary goal of gambling was to relieve economic hardship and improve cognitive-emotional state. All participants reported that they did not know when this goal was achieved. Start signals for gambling included the ideas and feelings that gambling could solve problems and sensations that it might be the right time to win. The stop signal for gambling, for all participants, was running out of money. All participants also reported a perceived reduction in self-consciousness during a gambling episode. These findings provide preliminary evidence that specific facets of metacognition play a role in gambling disorder.

  7. Continuous-time signals

    CERN Document Server

    Shmaliy, Yuriy

    2006-01-01

    Gives a modern description of continuous-time deterministic signals Signal formation techniquesTime vs. frequency and frequency vs. time analysisCorrelation and energy analysisNarrowband signals and sampling.

  8. GRB neutrino detection via time profile stacking

    CERN Document Server

    van Eijndhoven, Nick

    2007-01-01

    A method is presented for the identification of high-energy neutrinos from gamma ray bursts by means of a large-scale neutrino telescope. The procedure makes use of a time profile stacking technique of observed neutrino induced signals in correlation with satellite observations. By selecting a rather wide time window, a possible difference between the arrival times of the gamma and neutrino signals may also be identified. This might provide insight in the particle production processes at the source. By means of a toy model it will be demonstrated that a statistically significant signal can be obtained with a km$^{3}$-scale neutrino telescope on a sample of 500 gamma ray bursts for a signal rate as low as 1 detectable neutrino for 3% of the bursts.

  9. Basic digital signal processing

    CERN Document Server

    Lockhart, Gordon B

    1985-01-01

    Basic Digital Signal Processing describes the principles of digital signal processing and experiments with BASIC programs involving the fast Fourier theorem (FFT). The book reviews the fundamentals of the BASIC program, continuous and discrete time signals including analog signals, Fourier analysis, discrete Fourier transform, signal energy, power. The text also explains digital signal processing involving digital filters, linear time-variant systems, discrete time unit impulse, discrete-time convolution, and the alternative structure for second order infinite impulse response (IIR) sections.

  10. Extremal periodic wave profiles

    Directory of Open Access Journals (Sweden)

    E. van Groesen

    2007-01-01

    Full Text Available As a contribution to deterministic investigations into extreme fluid surface waves, in this paper wave profiles of prescribed period that have maximal crest height will be investigated. As constraints the values of the momentum and energy integrals are used in a simplified description with the KdV model. The result is that at the boundary of the feasible region in the momentum-energy plane, the only possible profiles are the well known cnoidal wave profiles. Inside the feasible region the extremal profiles of maximal crest height are "cornered" cnoidal profiles: cnoidal profiles of larger period, cut-off and periodically continued with the prescribed period so that at the maximal crest height a corner results.

  11. Ascaroside signaling in C. elegans.

    Science.gov (United States)

    Ludewig, Andreas H; Schroeder, Frank C

    2013-01-18

    Over the past 10 years, the relevance of small-molecule signaling for many aspects of C. elegans development and behavior has become apparent. One prominent group of small-molecule signals are the ascarosides, which control dauer entry and exit as well as a variety of sex-specific and social behaviors, including male attraction, hermaphrodite repulsion, olfactory plasticity, and aggregation. This wide range of biological functions is facilitated by a great diversity of ascaroside chemical structures. These are based on the sugar ascarylose, which is linked to fatty acid-like side chains of varying lengths and often decorated further with building blocks derived from amino acids, folate, and other primary metabolites. Different ascarosides or combinations of ascarosides mediate different phenotypes, and even small differences in chemical structures are often associated with strongly altered activity profiles. Additional complexity arises from concentration-dependent effects and synergism between different ascarosides. The ascarosides are sensed by several types of chemosensory head neurons, including the ASK, ASI, and ADL neurons as well as the male-specific CEM neurons. Ascaroside perception is mediated by diverse families of G-protein coupled membrane receptors that act upstream of conserved signal transduction pathways, including insulin/IGF-1 signaling and transforming growth factor beta (TGF-β) signaling. Biosynthesis of the ascarosides appears to integrate input from several primary metabolic pathways, including peroxisomal β-oxidation of long-chain fatty acids and amino acid catabolism. Life stage, sex, as well as food availability and other environmental factors affect ascaroside biosynthesis, suggesting that ascaroside signaling communicates detailed information about life history and metabolic state.

  12. Geometrical constraint experimental determination of Raman lidar overlap profile.

    Science.gov (United States)

    Li, Jian; Li, Chengcai; Zhao, Yiming; Li, Jing; Chu, Yiqi

    2016-06-20

    A simple experimental method to determine the overlap profile of Raman lidar is presented in this paper. Based on Mie and Raman backscattering signals and a geometrically constrained condition, the overlap profile of a Raman lidar system can be determined. Our approach simultaneously retrieves the lidar ratio of aerosols, which is one of the most important sources of uncertainty in the overlap profile determination. The results indicate that the overlap factor is significantly influenced by the lidar ratio in experimental methods. A representative case study indicates that the correction of the overlap profile obtained by this method is practical and feasible.

  13. YOUNG ATHLETES' MOTIVATIONAL PROFILES

    Directory of Open Access Journals (Sweden)

    Juan Antonio Moreno Murcia

    2007-06-01

    Full Text Available The aim of this study was to examine the relationship between motivational characteristics and dispositional flow. In order to accomplish this goal, motivational profiles emerging from key constructs within Achievement Goal Theory and Self-Determination Theory were related to the dispositional flow measures. A sample of 413 young athletes (Age range 12 to 16 years completed the PMCSQ-2, POSQ, SMS and DFS measures. Cluster analysis results revealed three profiles: a "self-determined profile" characterised by higher scores on the task-involving climate perception and on the task orientation; a "non-self-determined profile", characterised by higher scores on ego-involving climate perception and ego orientation; and a "low self-determined and low non-self-determined profile" which had the lowest dispositional flow. No meaningful differences were found between the "self-determined profile" and the "non-self-determined profile" in dispositional flow. The "self-determined profile" was more commonly associated with females, athletes practising individual sports and those training more than three days a week. The "non-self-determined profile" was more customary of males and athletes practising team sports as well as those training just two or three days a week

  14. Multiwavelength micropulse lidar in atmospheric aerosol study: signal processing

    Science.gov (United States)

    Posyniak, Michal; Malinowski, Szymon P.; Stacewicz, Tadeusz; Markowicz, Krzysztof M.; Zielinski, Tymon; Petelski, Tomasz; Makuch, Przemyslaw

    2011-11-01

    Multiwavelength micropulse lidar (MML) designed for continuous optical sounding of the atmosphere is presented. A specific signal processing technique applying two directional Kalman filtering is introduced in order to enhance signal to noise ratio. Application of this technique is illustrated with profiles collected in course of COAST 2009 and WRNP 2010 research campaigns.

  15. Interactive Digital Signal Processor

    Science.gov (United States)

    Mish, W. H.

    1985-01-01

    Interactive Digital Signal Processor, IDSP, consists of set of time series analysis "operators" based on various algorithms commonly used for digital signal analysis. Processing of digital signal time series to extract information usually achieved by applications of number of fairly standard operations. IDSP excellent teaching tool for demonstrating application for time series operators to artificially generated signals.

  16. Signals and processing for random signal radars

    Science.gov (United States)

    Moore, G. S.

    1980-06-01

    Signals and associated processing techniques are developed which improve the performance, simplify the implementation, and are more amenable to adaptive operation for radars using the random signal concept. These goals are accomplished through the use of a signal set that is composed of a deterministic spreading function, a binary random or pseudo-random noise source, and a possibly random or pseudo-random pulsing sequence. Techniques are developed for determining the parameters of the spreading function that result in signals with desirable ambiguity functions and high effective power. These techniques are based on the use of window functions for sidelobe control and the theory of chirp waveforms for effective power enhancement.

  17. Spiking the expectancy profiles

    DEFF Research Database (Denmark)

    Hansen, Niels Chr.; Loui, Psyche; Vuust, Peter

    Melodic expectations have long been quantified using expectedness ratings. Motivated by statistical learning and sharper key profiles in musicians, we model musical learning as a process of reducing the relative entropy between listeners' prior expectancy profiles and probability distributions...... learning over varying timescales enables listeners to generate expectations with reduced entropy....

  18. Household electricity demand profiles

    DEFF Research Database (Denmark)

    Marszal, Anna Joanna; Heiselberg, Per Kvols; Larsen, Olena Kalyanova

    2016-01-01

    Highlights •A 1-min resolution household electricity load model is presented. •Model adapts a bottom-up approach with single appliance as the main building block. •Load profiles are used to analyse the flexibility potential of household appliances. •Load profiles can be applied in other domains, e...

  19. Chemical profiling of explosives

    NARCIS (Netherlands)

    Brust, G.M.H.

    2014-01-01

    The primary goal of this thesis is to develop analytical methods for the chemical profiling of explosives. Current methodologies for the forensic analysis of explosives focus on identification of the explosive material. However, chemical profiling of explosives becomes increasingly important, as

  20. Reinforced aerodynamic profile

    DEFF Research Database (Denmark)

    2010-01-01

    The present invention relates to the prevention of deformations in an aerodynamic profile caused by lack of resistance to the bending moment forces that are created when such a profile is loaded in operation. More specifically, the invention relates to a reinforcing element inside an aerodynamic...

  1. Digital signal processing laboratory

    CERN Document Server

    Kumar, B Preetham

    2011-01-01

    INTRODUCTION TO DIGITAL SIGNAL PROCESSING Brief Theory of DSP ConceptsProblem SolvingComputer Laboratory: Introduction to MATLAB®/SIMULINK®Hardware Laboratory: Working with Oscilloscopes, Spectrum Analyzers, Signal SourcesDigital Signal Processors (DSPs)ReferencesDISCRETE-TIME LTI SIGNALS AND SYSTEMS Brief Theory of Discrete-Time Signals and SystemsProblem SolvingComputer Laboratory: Simulation of Continuous Time and Discrete-Time Signals and Systems ReferencesTIME AND FREQUENCY ANALYSIS OF COMMUNICATION SIGNALS Brief Theory of Discrete-Time Fourier Transform (DTFT), Discrete Fourier Transform

  2. Multiplexing oscillatory biochemical signals.

    Science.gov (United States)

    de Ronde, Wiet; ten Wolde, Pieter Rein

    2014-04-01

    In recent years it has been increasingly recognized that biochemical signals are not necessarily constant in time and that the temporal dynamics of a signal can be the information carrier. Moreover, it is now well established that the protein signaling network of living cells has a bow-tie structure and that components are often shared between different signaling pathways. Here we show by mathematical modeling that living cells can multiplex a constant and an oscillatory signal: they can transmit these two signals simultaneously through a common signaling pathway, and yet respond to them specifically and reliably. We find that information transmission is reduced not only by noise arising from the intrinsic stochasticity of biochemical reactions, but also by crosstalk between the different channels. Yet, under biologically relevant conditions more than 2 bits of information can be transmitted per channel, even when the two signals are transmitted simultaneously. These observations suggest that oscillatory signals are ideal for multiplexing signals.

  3. Profiling the Mobile Customer

    DEFF Research Database (Denmark)

    Jessen, Pernille Wegener; King, Nancy J.

    2010-01-01

    of significant concerns about privacy and data protection. This second article in a two part series on "Profiling the Mobile Customer" explores how to best protect consumers' privacy and personal data through available mechanisms that include industry self-regulation, privacy-enhancing technologies......Mobile customers are increasingly being tracked and profiled by behavioural advertisers to enhance delivery of personalized advertising. This type of profiling relies on automated processes that mine databases containing personally-identifying or anonymous consumer data, and it raises a host...... discusses the current limitations of using technology to protect consumers from privacy abuses related to profiling. Concluding that industry self-regulation and available privacy-enhancing technologies will not be adequate to close important privacy gaps related to consumer profiling without legislative...

  4. Full profile incoherent scatter analysis at Jicamarca

    Directory of Open Access Journals (Sweden)

    D. L. Hysell

    2008-02-01

    Full Text Available Incoherent scatter data from a hybrid long-pulse/double-pulse experiment at Jicamarca are analyzed using a full-profile analysis similar to the one implemented by Holt et al. (1992. In this case, plasma density, electron and ion temperatures, and light ion composition profiles in the topside are estimated simultaneously. Full-profile analysis is crucial at Jicamarca, since the long correlation time of the incoherent scatter signal at 50 MHz invalidates conventional gated analysis. Results for a 24 h interval in April of 2006 are presented, covering altitudes through 1600 km with 10 min time resolution, and compared with results from the NRL ionospheric model SAMI2. The analysis provides the first comprehensive assessment of ionospheric conditions over Jicamarca at sunrise as well as the first 24-h record of helium ion layers. Possible refinements to the experiment and the algorithm are discussed.

  5. Beam profile analyzer for CO2 lasers

    Directory of Open Access Journals (Sweden)

    Rubén López

    2015-12-01

    Full Text Available The development of an optoelectronic system to analyze the beam intensity profile of CO2 lasers is presented herein. The device collects the beam profile with a LiTaO3 pyroelectric detector and uses a sampling technique based on the acquisition of horizontal sections at different levels. The digital signal processing includes subroutines that drop down two dimensional and three dimensional beam profile displays to determine the laser beam parameters of optical power, peak pixel location, centroid location and width of the laser beam, with algorithms based on the ISO 11146 standard. With the systematic calibration of the analyzer was obtained in the measurement of power an error under 5%, for a 20–200 W range and an error under 1.6% for spatial measurements of a TEM00 laser. By design, the analyzer can be used during the laser process.

  6. Estimating Cognitive Profiles Using Profile Analysis via Multidimensional Scaling (PAMS)

    Science.gov (United States)

    Kim, Se-Kang; Frisby, Craig L.; Davison, Mark L.

    2004-01-01

    Two of the most popular methods of profile analysis, cluster analysis and modal profile analysis, have limitations. First, neither technique is adequate when the sample size is large. Second, neither method will necessarily provide profile information in terms of both level and pattern. A new method of profile analysis, called Profile Analysis via…

  7. Multidimensional signal processing for ultrasonic signal classification

    Science.gov (United States)

    Kim, J.; Ramuhalli, P.; Udpa, L.; Udpa, S.

    2001-04-01

    Neural network based signal classification systems are being used increasingly in the analysis of large volumes of data obtained in NDE applications. One example is in the interpretation on ultrasonic signals obtained from inspection of welds where signals can be due to porosity, slag, lack of fusion and cracks in the weld region. Standard techniques rely on differences in individual A-scans to classify the signals. This paper proposes an ultrasonic signal classification technique based on the information in a group of signals and examining the statistical characteristics of the signals. The method was 2-dimensional signal processing algorithms to analyze the information in B- and B'-scan images. In this paper, 2-dimensional transform based coefficients of the images are used as features and a multilayer perceptron is used to classify them. These results are then combined to get the final classification for the inspected region. Results of applying the technique to data obtained from the inspection of welds are presented.

  8. Surveys, Astrometric Follow-up & Population Statistics

    CERN Document Server

    Jedicke, Robert; Micheli, Marco; Ryan, Eileen; Spahr, Timothy; Yeomans, Donald K

    2015-01-01

    Asteroid surveys are the backbone of asteroid science, and with this in mind we begin with a broad review of the impact of asteroid surveys on our field. We then provide a brief history of asteroid discoveries so as to place contemporary and future surveys in perspective. Surveys in the United States have discovered the vast majority of the asteroids and this dominance has been consolidated since the publication of Asteroids III. Our descriptions of the asteroid surveys that have been operational since that time are focussed upon those that have contributed the vast majority of asteroid observations and discoveries. We also provide some insight into upcoming next-generation surveys that are sure to alter our understanding of the small bodies in the inner solar system and provide evidence to untangle their complicated dynamical and physical histories. The Minor Planet Center, the nerve center of the asteroid discovery effort, has improved its operations significantly in the past decade so that it can manage th...

  9. Astrometric Determination of VSOP-2 Orbital Parameters

    Science.gov (United States)

    Fomalont, E.; Moellenbrock, G.; Claussen, M.

    2009-08-01

    VSOP-2 phase referencing, needed to image faint sources and to determine accurate positions, will require an orbit accuracy of about 2 cm at 23 GHz. This accuracy, however, may not be obtainable by direct orbital measurements. We propose an observation scheme, similar to that used at the VLBA, to be included during a phase referencing observation of about one orbit in order to determine a more accurate orbit determination. We show the effects of orbit errors, explain the suggested observations and reduction methods, and discuss the potential problems that might impede the use of this technique.

  10. VSOP-2 Astrometric Accuracy with Phase Referencing

    Science.gov (United States)

    Asaki, Y.; Kono, Y.

    2009-08-01

    The VSOP-2 mission is expected to conduct phase referencing observations with the unprecedented spatial resolutions at 8.4, 22, and 43 GHz together with the ASTRO-G satellite. In this report, VSOP-2 astrometry with phase referencing is examined in detail based on a simulation tool, ARIS.

  11. Network modeling reveals prevalent negative regulatory relationships between signaling sectors in Arabidopsis immune signaling.

    Directory of Open Access Journals (Sweden)

    Masanao Sato

    Full Text Available Biological signaling processes may be mediated by complex networks in which network components and network sectors interact with each other in complex ways. Studies of complex networks benefit from approaches in which the roles of individual components are considered in the context of the network. The plant immune signaling network, which controls inducible responses to pathogen attack, is such a complex network. We studied the Arabidopsis immune signaling network upon challenge with a strain of the bacterial pathogen Pseudomonas syringae expressing the effector protein AvrRpt2 (Pto DC3000 AvrRpt2. This bacterial strain feeds multiple inputs into the signaling network, allowing many parts of the network to be activated at once. mRNA profiles for 571 immune response genes of 22 Arabidopsis immunity mutants and wild type were collected 6 hours after inoculation with Pto DC3000 AvrRpt2. The mRNA profiles were analyzed as detailed descriptions of changes in the network state resulting from the genetic perturbations. Regulatory relationships among the genes corresponding to the mutations were inferred by recursively applying a non-linear dimensionality reduction procedure to the mRNA profile data. The resulting static network model accurately predicted 23 of 25 regulatory relationships reported in the literature, suggesting that predictions of novel regulatory relationships are also accurate. The network model revealed two striking features: (i the components of the network are highly interconnected; and (ii negative regulatory relationships are common between signaling sectors. Complex regulatory relationships, including a novel negative regulatory relationship between the early microbe-associated molecular pattern-triggered signaling sectors and the salicylic acid sector, were further validated. We propose that prevalent negative regulatory relationships among the signaling sectors make the plant immune signaling network a "sector

  12. Qualitative Value Profiling

    DEFF Research Database (Denmark)

    Duus, Henrik Johannsen; Bjerre, Mogens

    2015-01-01

    Qualitative value profiling (QVP) is a relatively unknown method of strategic analysis for companies in international business-to-business settings. The purpose of QVP is to reduce the information complexity that is faced by international companies in dealing with business partners. The QVP method...... allows the development of 1) profiles of the target country in which operations are to take place, 2) profiles of the buying center (i.e. the group of decision makers) in the partner company, and 3) profiles of the product/service offering. It also allows the development of a semantic scaling method...... for deeper analysis of all involved factors. This paper presents the method and compares and contrasts it with other similar methods like the PESTELE method known from corporate strategy, the STEEPAL method known from scenario analysis, and the Politics-Institutions-Economy (PIE) framework known from...

  13. Profile of Older Americans

    Science.gov (United States)

    ... covers 15 topical areas, including population, income and poverty, living arrangements, education, health, and caregiving. A Profile ... Elder Justice Innovation Grants Late Life Domestic Violence World Elder Abuse Awareness Day State Grants to Enhance ...

  14. Prescription Drug Profiles PUF

    Data.gov (United States)

    U.S. Department of Health & Human Services — This release contains the Prescription Drug Profiles Public Use Files (PUFs) drawn from Medicare prescription drug claims for the year of the date on which the...

  15. Beach Profile Locations

    Data.gov (United States)

    California Department of Resources — Beaches are commonly characterized by cross-shore surveys. The resulting profiles represent the elevation of the beach surface and nearshore seabed from the back of...

  16. Fishing Community Profiles

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — To enable fisheries managers to comply with National Standard 8 (NS8), NMFS social scientists around the nation are preparing fishing community profiles that present...

  17. Profiles in Cancer Research

    Science.gov (United States)

    These articles put a face to some of the thousands of individuals who contribute to NCI’s cancer research efforts. The profiles highlight the work of scientists and clinicians and describe the circumstances and motivation behind their work.

  18. Profiling the Mobile Customer

    DEFF Research Database (Denmark)

    Jessen, Pernille Wegener; King, Nancy J.

    2010-01-01

    Mobile customers are increasingly being tracked and profiled by behavioural advertisers to enhance delivery of personalized advertising. This type of profiling relies on automated processes that mine databases containing personally-identifying or anonymous consumer data, and it raises a host...... of significant concerns about privacy and data protection. This second article in a two part series on "Profiling the Mobile Customer" explores how to best protect consumers' privacy and personal data through available mechanisms that include industry self-regulation, privacy-enhancing technologies...... and legislative reform. 1 It discusses how well privacy and personal data concerns related to consumer profiling are addressed by two leading industry self-regulatory codes from the UK and the U.S. that aim to establish fair information practices for behavioural advertising by their member companies. It also...

  19. Fire Management Species Profiles

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The objective of the Fire Management Species Profile project is to identify habitat management objectives that are specific, measurable, achievable, clearly...

  20. Beach Profile Locations

    Data.gov (United States)

    California Department of Resources — Beaches are commonly characterized by cross-shore surveys. The resulting profiles represent the elevation of the beach surface and nearshore seabed from the back of...

  1. FIST: a sensory domain for diverse signal transduction pathways in prokaryotes and ubiquitin signaling in eukaryotes

    Energy Technology Data Exchange (ETDEWEB)

    Borziak, Kirill [ORNL; Jouline, Igor B [ORNL

    2007-01-01

    Motivation: Sensory domains that are conserved among Bacteria, Archaea and Eucarya are important detectors of common signals detected by living cells. Due to their high sequence divergence, sensory domains are difficult to identify. We systematically look for novel sensory domains using sensitive profile-based searches initi-ated with regions of signal transduction proteins where no known domains can be identified by current domain models. Results: Using profile searches followed by multiple sequence alignment, structure prediction, and domain architecture analysis, we have identified a novel sensory domain termed FIST, which is present in signal transduction proteins from Bacteria, Archaea and Eucarya. Remote similarity to a known ligand-binding fold and chromosomal proximity of FIST-encoding genes to those coding for proteins involved in amino acid metabolism and transport suggest that FIST domains bind small ligands, such as amino acids.

  2. BWR AXIAL PROFILE

    Energy Technology Data Exchange (ETDEWEB)

    J. Huffer

    2004-09-28

    The purpose of this calculation is to develop axial profiles for estimating the axial variation in burnup of a boiling water reactor (BWR) assembly spent nuclear fuel (SNF) given the average burnup of an assembly. A discharged fuel assembly typically exhibits higher burnup in the center and lower burnup at the ends of the assembly. Criticality safety analyses taking credit for SNF burnup must account for axially varying burnup relative to calculations based on uniformly distributed assembly average burnup due to the under-burned tips. Thus, accounting for axially varying burnup in criticality analyses is also referred to as accounting for the ''end effect'' reactivity. The magnitude of the reactivity change due to ''end effect'' is dependent on the initial assembly enrichment, the assembly average burnup, and the particular axial profile characterizing the burnup distribution. The set of bounding axial profiles should incorporate multiple BWR core designs and provide statistical confidence (95 percent confidence that 95 percent of the population is bound by the profile) that end nodes are conservatively represented. The profiles should also conserve the overall burnup of the fuel assembly. More background on BWR axial profiles is provided in Attachment I.

  3. Accelerated Profile HMM Searches.

    Directory of Open Access Journals (Sweden)

    Sean R Eddy

    2011-10-01

    Full Text Available Profile hidden Markov models (profile HMMs and probabilistic inference methods have made important contributions to the theory of sequence database homology search. However, practical use of profile HMM methods has been hindered by the computational expense of existing software implementations. Here I describe an acceleration heuristic for profile HMMs, the "multiple segment Viterbi" (MSV algorithm. The MSV algorithm computes an optimal sum of multiple ungapped local alignment segments using a striped vector-parallel approach previously described for fast Smith/Waterman alignment. MSV scores follow the same statistical distribution as gapped optimal local alignment scores, allowing rapid evaluation of significance of an MSV score and thus facilitating its use as a heuristic filter. I also describe a 20-fold acceleration of the standard profile HMM Forward/Backward algorithms using a method I call "sparse rescaling". These methods are assembled in a pipeline in which high-scoring MSV hits are passed on for reanalysis with the full HMM Forward/Backward algorithm. This accelerated pipeline is implemented in the freely available HMMER3 software package. Performance benchmarks show that the use of the heuristic MSV filter sacrifices negligible sensitivity compared to unaccelerated profile HMM searches. HMMER3 is substantially more sensitive and 100- to 1000-fold faster than HMMER2. HMMER3 is now about as fast as BLAST for protein searches.

  4. Cell signaling review series

    Institute of Scientific and Technical Information of China (English)

    Aiming Lin; Zhenggang Liu

    2008-01-01

    @@ Signal transduction is pivotal for many, if not all, fundamental cellular functions including proliferation, differentiation, transformation and programmed cell death. Deregulation of cell signaling may result in certain types of cancers and other human diseases.

  5. Retinoid signalling during embryogenesis

    NARCIS (Netherlands)

    Pijnappel, W.W.M.; Hendriks, H.F.J.; Durston, A.J.

    1996-01-01

    Conclusion: Retinoids are suspected to have multiple functions during embryogenesis, which are carried out via various different signal transduction pathways involving active retinoids and nuclear retinoid receptors. Research focuses on the identification of the retinoid signal transduction componen

  6. Biomedical signal processing

    CERN Document Server

    Akay, Metin

    1994-01-01

    Sophisticated techniques for signal processing are now available to the biomedical specialist! Written in an easy-to-read, straightforward style, Biomedical Signal Processing presents techniques to eliminate background noise, enhance signal detection, and analyze computer data, making results easy to comprehend and apply. In addition to examining techniques for electrical signal analysis, filtering, and transforms, the author supplies an extensive appendix with several computer programs that demonstrate techniques presented in the text.

  7. Danger signals in stroke.

    Science.gov (United States)

    Gelderblom, Mathias; Sobey, Christopher G; Kleinschnitz, Christoph; Magnus, Tim

    2015-11-01

    Danger molecules are the first signals released from dying tissue after stroke. These danger signals bind to receptors on immune cells that will result in their activation and the release of inflammatory and neurotoxic mediators, resulting in amplification of the immune response and subsequent enlargement of the damaged brain volume. The release of danger signals is a central event that leads to a multitude of signals and cascades in the affected and neighbouring tissue, therefore providing a potential target for therapy.

  8. Tetrapyrrole Signaling in Plants

    Science.gov (United States)

    Larkin, Robert M.

    2016-01-01

    Tetrapyrroles make critical contributions to a number of important processes in diverse organisms. In plants, tetrapyrroles are essential for light signaling, the detoxification of reactive oxygen species, the assimilation of nitrate and sulfate, respiration, photosynthesis, and programed cell death. The misregulation of tetrapyrrole metabolism can produce toxic reactive oxygen species. Thus, it is not surprising that tetrapyrrole metabolism is strictly regulated and that tetrapyrrole metabolism affects signaling mechanisms that regulate gene expression. In plants and algae, tetrapyrroles are synthesized in plastids and were some of the first plastid signals demonstrated to regulate nuclear gene expression. In plants, the mechanism of tetrapyrrole-dependent plastid-to-nucleus signaling remains poorly understood. Additionally, some of experiments that tested ideas for possible signaling mechanisms appeared to produce conflicting data. In some instances, these conflicts are potentially explained by different experimental conditions. Although the biological function of tetrapyrrole signaling is poorly understood, there is compelling evidence that this signaling is significant. Specifically, this signaling appears to affect the accumulation of starch and may promote abiotic stress tolerance. Tetrapyrrole-dependent plastid-to-nucleus signaling interacts with a distinct plastid-to-nucleus signaling mechanism that depends on GENOMES UNCUOPLED1 (GUN1). GUN1 contributes to a variety of processes, such as chloroplast biogenesis, the circadian rhythm, abiotic stress tolerance, and development. Thus, the contribution of tetrapyrrole signaling to plant function is potentially broader than we currently appreciate. In this review, I discuss these aspects of tetrapyrrole signaling.

  9. Acoustic Signals and Systems

    DEFF Research Database (Denmark)

    2008-01-01

    The Handbook of Signal Processing in Acoustics will compile the techniques and applications of signal processing as they are used in the many varied areas of Acoustics. The Handbook will emphasize the interdisciplinary nature of signal processing in acoustics. Each Section of the Handbook will pr...

  10. ACP with signals

    NARCIS (Netherlands)

    Bergstra, J.A.

    1988-01-01

    New operators are introduced on top of ACP [BK 84] in order to incorporate stable signals in process algebra. Semantically this involves assigning labels to nodes of process graphs. The labels of nodes are called signals. In combination with the operators of BPA, a signal insertion operator

  11. Optimal Signal Quality Index for Photoplethysmogram Signals

    Directory of Open Access Journals (Sweden)

    Mohamed Elgendi

    2016-09-01

    Full Text Available A photoplethysmogram (PPG is a noninvasive circulatory signal related to the pulsatile volume of blood in tissue and is typically collected by pulse oximeters. PPG signals collected via mobile devices are prone to artifacts that negatively impact measurement accuracy, which can lead to a significant number of misleading diagnoses. Given the rapidly increased use of mobile devices to collect PPG signals, developing an optimal signal quality index (SQI is essential to classify the signal quality from these devices. Eight SQIs were developed and tested based on: perfusion, kurtosis, skewness, relative power, non-stationarity, zero crossing, entropy, and the matching of systolic wave detectors. Two independent annotators annotated all PPG data (106 recordings, 60 s each and a third expert conducted the adjudication of differences. The independent annotators labeled each PPG signal with one of the following labels: excellent, acceptable or unfit for diagnosis. All indices were compared using Mahalanobis distance, linear discriminant analysis, quadratic discriminant analysis, and support vector machine with leave-one-out cross-validation. The skewness index outperformed the other seven indices in differentiating between excellent PPG and acceptable, acceptable combined with unfit, and unfit recordings, with overall F 1 scores of 86.0%, 87.2%, and 79.1%, respectively.

  12. Titan Extinction Profiles Observed by Cassini Radio Occultations and Implications

    Science.gov (United States)

    Marouf, Essam; French, Richard; Flasar, F. Michael; Schinder, Paul J.; Rappaport, Nicole J.

    Three monochromatic and phase-coherent radio signals of wavelength = 0.9, 3.6, and 13 cm (Ka-, X-, and S-bands), were propagated by Cassini through the neutral atmosphere of Titan and the refracted signals were observed on the Earth. Abel inversion of observed changes in the signal frequency is used to recover the refractivity profile of the atmosphere, hence estimate the expected loss in signal strength due to defocusing of the radio signal by differential refraction. The refractive defocusing component (wavelength independent, in principle) is then removed from the actual measured signal strength profiles yielding the "true" signal extinction due to absorption and scattering integrated along the propagation path. Abel inversion of the integrated intensity profiles, tempered to combat noise contribution, yields localized estimates of the extinction coefficient (absorbtivity) as a function of altitude, or the extinction profiles. The initial radio measurements are diffraction-limited. We extend Fresnel transform based diffraction reconstruction procedures developed for radio occultation observations of planetary rings to remove diffraction effects from the initial radio measurements. The procedures are tested using idealized models of simple isothermal atmospheric profile extending above a hard-limb (knife-edge) model. Reconstruction of the simulated "observed" diffraction-limited data shows good agreement with the assumed atmospheric profile and the location of the hard-limb for a range of model parameters. We then apply a similar approach to the actual measured data. Strong wavelength-dependent extinction coefficient profile behavior is observed. Its large-scale structure appears well modeled by predictions based on N2-N2 collision-induced gaseous absorption for Titan's physical conditions. Interesting localized features of yet unexplained origin are also observed. Because the spatial scales of the extinction profile features are relatively large compared with

  13. One laser pulse generates two photoacoustic signals

    OpenAIRE

    Gao, Fei; Feng, Xiaohua; Bai, Linyi; Zhang, Ruochong; Liu, Siyu; Ding, Ran; Kishor, Rahul; Zhao, Yanli; Zheng, Yuanjin

    2016-01-01

    Photoacoustic sensing and imaging techniques have been studied widely to explore optical absorption contrast based on nanosecond laser illumination. In this paper, we report a long laser pulse induced dual photoacoustic (LDPA) nonlinear effect, which originates from unsatisfied stress and thermal confinements. Being different from conventional short laser pulse illumination, the proposed method utilizes a long square-profile laser pulse to induce dual photoacoustic signals. Without satisfying...

  14. Acoustic Signals and Systems

    DEFF Research Database (Denmark)

    2008-01-01

    The Handbook of Signal Processing in Acoustics will compile the techniques and applications of signal processing as they are used in the many varied areas of Acoustics. The Handbook will emphasize the interdisciplinary nature of signal processing in acoustics. Each Section of the Handbook...... will present topics on signal processing which are important in a specific area of acoustics. These will be of interest to specialists in these areas because they will be presented from their technical perspective, rather than a generic engineering approach to signal processing. Non-specialists, or specialists...

  15. Acoustic Signal Processing

    Science.gov (United States)

    Hartmann, William M.; Candy, James V.

    Signal processing refers to the acquisition, storage, display, and generation of signals - also to the extraction of information from signals and the re-encoding of information. As such, signal processing in some form is an essential element in the practice of all aspects of acoustics. Signal processing algorithms enable acousticians to separate signals from noise, to perform automatic speech recognition, or to compress information for more efficient storage or transmission. Signal processing concepts are the building blocks used to construct models of speech and hearing. Now, in the 21st century, all signal processing is effectively digital signal processing. Widespread access to high-speed processing, massive memory, and inexpensive software make signal processing procedures of enormous sophistication and power available to anyone who wants to use them. Because advanced signal processing is now accessible to everybody, there is a need for primers that introduce basic mathematical concepts that underlie the digital algorithms. The present handbook chapter is intended to serve such a purpose.

  16. Reliable Signal Transduction

    Science.gov (United States)

    Wollman, Roy

    Stochasticity inherent to biochemical reactions (intrinsic noise) and variability in cellular states (extrinsic noise) degrade information transmitted through signaling networks. We analyzed the ability of temporal signal modulation - that is dynamics - to reduce noise-induced information loss. In the extracellular signal-regulated kinase (ERK), calcium (Ca(2 +)) , and nuclear factor kappa-B (NF- κB) pathways, response dynamics resulted in significantly greater information transmission capacities compared to nondynamic responses. Theoretical analysis demonstrated that signaling dynamics has a key role in overcoming extrinsic noise. Experimental measurements of information transmission in the ERK network under varying signal-to-noise levels confirmed our predictions and showed that signaling dynamics mitigate, and can potentially eliminate, extrinsic noise-induced information loss. By curbing the information-degrading effects of cell-to-cell variability, dynamic responses substantially increase the accuracy of biochemical signaling networks.

  17. Second-hand signals

    DEFF Research Database (Denmark)

    Bergenholtz, Carsten

    2014-01-01

    Studies of signaling theory have traditionally focused on the dyadic link between the sender and receiver of the signal. Within a science‐based perspective this framing has led scholars to investigate how patents and publications of firms function as signals. I explore another important type...... of signal of firms, which is based on a formalized common practice of external, academic experts referring to firms in their peer reviewed publications. The findings provide qualitative evidence that helps explain why and how this new type of ‘second‐hand’ signal is created, validated and systematically...... used by various agents in their search for and assessment of products and firms. I conclude by arguing how this second‐hand nature of signals goes beyond a simple dyadic focus on senders and receivers of signals, and thus elucidates the more complex interrelations of the various types of agents...

  18. Adaptive Sensing Based on Profiles for Sensor Systems

    Directory of Open Access Journals (Sweden)

    Yoshiteru Ishida

    2009-10-01

    Full Text Available This paper proposes a profile-based sensing framework for adaptive sensor systems based on models that relate possibly heterogeneous sensor data and profiles generated by the models to detect events. With these concepts, three phases for building the sensor systems are extracted from two examples: a combustion control sensor system for an automobile engine, and a sensor system for home security. The three phases are: modeling, profiling, and managing trade-offs. Designing and building a sensor system involves mapping the signals to a model to achieve a given mission.

  19. Country profile: Hungary

    Energy Technology Data Exchange (ETDEWEB)

    1991-09-01

    Country Profile: Hungary has been prepared as a background document for use by US Government agencies and US businesses interested in becoming involved with the new democracies of Eastern Europe as they pursue sustainable economic development. The focus of the Profile is on energy and highlights information on Hungary's energy supply, demand, and utilization. It identifies patterns of energy usage in the important economic sectors, especially industry, and provides a preliminary assessment for opportunities to improve efficiencies in energy production, distribution and use by introducing more efficient technologies. The use of more efficient technologies would have the added benefit of reducing the environmental impact which, although is not the focus of the report, is an issue that effects energy choices. The Profile also presents considerable economic information, primarily in the context of how economic restructuring may affect energy supply, demand, and the introduction of more efficient technologies.

  20. Country profile: Hungary

    Energy Technology Data Exchange (ETDEWEB)

    1991-09-01

    Country Profile: Hungary has been prepared as a background document for use by US Government agencies and US businesses interested in becoming involved with the new democracies of Eastern Europe as they pursue sustainable economic development. The focus of the Profile is on energy and highlights information on Hungary`s energy supply, demand, and utilization. It identifies patterns of energy usage in the important economic sectors, especially industry, and provides a preliminary assessment for opportunities to improve efficiencies in energy production, distribution and use by introducing more efficient technologies. The use of more efficient technologies would have the added benefit of reducing the environmental impact which, although is not the focus of the report, is an issue that effects energy choices. The Profile also presents considerable economic information, primarily in the context of how economic restructuring may affect energy supply, demand, and the introduction of more efficient technologies.

  1. Detonation Wave Profile

    Energy Technology Data Exchange (ETDEWEB)

    Menikoff, Ralph [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-12-14

    The Zel’dovich-von Neumann-Doering (ZND) profile of a detonation wave is derived. Two basic assumptions are required: i. An equation of state (EOS) for a partly burned explosive; P(V, e, λ). ii. A burn rate for the reaction progress variable; d/dt λ = R(V, e, λ). For a steady planar detonation wave the reactive flow PDEs can be reduced to ODEs. The detonation wave profile can be determined from an ODE plus algebraic equations for points on the partly burned detonation loci with a specified wave speed. Furthermore, for the CJ detonation speed the end of the reaction zone is sonic. A solution to the reactive flow equations can be constructed with a rarefaction wave following the detonation wave profile. This corresponds to an underdriven detonation wave, and the rarefaction is know as a Taylor wave.

  2. Constructing Data Curation Profiles

    Directory of Open Access Journals (Sweden)

    Michael Witt

    2009-12-01

    Full Text Available This paper presents a brief literature review and then introduces the methods, design, and construction of the Data Curation Profile, an instrument that can be used to provide detailed information on particular data forms that might be curated by an academic library. These data forms are presented in the context of the related sub-disciplinary research area, and they provide the flow of the research process from which these data are generated. The profiles also represent the needs for data curation from the perspective of the data producers, using their own language. As such, they support the exploration of data curation across different research domains in real and practical terms. With the sponsorship of the Institute of Museum and Library Services, investigators from Purdue University and the University of Illinois interviewed 19 faculty subjects to identify needs for discovery, access, preservation, and reuse of their research data. For each subject, a profile was constructed that includes information about his or her general research, data forms and stages, value of data, data ingest, intellectual property, organization and description of data, tools, interoperability, impact and prestige, data management, and preservation. Each profile also presents a specific dataset supplied by the subject to serve as a concrete example. The Data Curation Profiles are being published to a public wiki for questions and discussion, and a blank template will be disseminated with guidelines for others to create and share their own profiles. This study was conducted primarily from the viewpoint of librarians interacting with faculty researchers; however, it is expected that these findings will complement a wide variety of data curation research and practice outside of librarianship and the university environment.

  3. Global electricity company profiles

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-12-31

    Global Electricity Company Profiles examines the operations and strategies of 60 of the leading electricity companies in the world. Through its analysis of the key players, the report provides a comprehensive review of the current state of the global electricity industry. Using electricity sales as its criteria, the most successful companies are compared in terms of their national, regional and global importance. Global Electricity Company Profiles provides an outline of each company, its structure and operations, as well as key financial data. The detailed performance benchmarking and review of expectations for future development for each individual company provide a wealth of invaluable strategic information not available elsewhere. (author)

  4. Quantitation of signal transduction.

    Science.gov (United States)

    Krauss, S; Brand, M D

    2000-12-01

    Conventional qualitative approaches to signal transduction provide powerful ways to explore the architecture and function of signaling pathways. However, at the level of the complete system, they do not fully depict the interactions between signaling and metabolic pathways and fail to give a manageable overview of the complexity that is often a feature of cellular signal transduction. Here, we introduce a quantitative experimental approach to signal transduction that helps to overcome these difficulties. We present a quantitative analysis of signal transduction during early mitogen stimulation of lymphocytes, with steady-state respiration rate as a convenient marker of metabolic stimulation. First, by inhibiting various key signaling pathways, we measure their relative importance in regulating respiration. About 80% of the input signal is conveyed via identifiable routes: 50% through pathways sensitive to inhibitors of protein kinase C and MAP kinase and 30% through pathways sensitive to an inhibitor of calcineurin. Second, we quantify how each of these pathways differentially stimulates functional units of reactions that produce and consume a key intermediate in respiration: the mitochondrial membrane potential. Both the PKC and calcineurin routes stimulate consumption more strongly than production, whereas the unidentified signaling routes stimulate production more than consumption, leading to no change in membrane potential despite increased respiration rate. The approach allows a quantitative description of the relative importance of signal transduction pathways and the routes by which they activate a specific cellular process. It should be widely applicable.

  5. Automated modelling of signal transduction networks

    Directory of Open Access Journals (Sweden)

    Aach John

    2002-11-01

    Full Text Available Abstract Background Intracellular signal transduction is achieved by networks of proteins and small molecules that transmit information from the cell surface to the nucleus, where they ultimately effect transcriptional changes. Understanding the mechanisms cells use to accomplish this important process requires a detailed molecular description of the networks involved. Results We have developed a computational approach for generating static models of signal transduction networks which utilizes protein-interaction maps generated from large-scale two-hybrid screens and expression profiles from DNA microarrays. Networks are determined entirely by integrating protein-protein interaction data with microarray expression data, without prior knowledge of any pathway intermediates. In effect, this is equivalent to extracting subnetworks of the protein interaction dataset whose members have the most correlated expression profiles. Conclusion We show that our technique accurately reconstructs MAP Kinase signaling networks in Saccharomyces cerevisiae. This approach should enhance our ability to model signaling networks and to discover new components of known networks. More generally, it provides a method for synthesizing molecular data, either individual transcript abundance measurements or pairwise protein interactions, into higher level structures, such as pathways and networks.

  6. Metabolic signals in sleep regulation: recent insights.

    Science.gov (United States)

    Shukla, Charu; Basheer, Radhika

    2016-01-01

    Sleep and energy balance are essential for health. The two processes act in concert to regulate central and peripheral homeostasis. During sleep, energy is conserved due to suspended activity, movement, and sensory responses, and is redirected to restore and replenish proteins and their assemblies into cellular structures. During wakefulness, various energy-demanding activities lead to hunger. Thus, hunger promotes arousal, and subsequent feeding, followed by satiety that promotes sleep via changes in neuroendocrine or neuropeptide signals. These signals overlap with circuits of sleep-wakefulness, feeding, and energy expenditure. Here, we will briefly review the literature that describes the interplay between the circadian system, sleep-wake, and feeding-fasting cycles that are needed to maintain energy balance and a healthy metabolic profile. In doing so, we describe the neuroendocrine, hormonal/peptide signals that integrate sleep and feeding behavior with energy metabolism.

  7. Processing oscillatory signals by incoherent feedforward loops

    Science.gov (United States)

    Zhang, Carolyn; Wu, Feilun; Tsoi, Ryan; Shats, Igor; You, Lingchong

    From the timing of amoeba development to the maintenance of stem cell pluripotency,many biological signaling pathways exhibit the ability to differentiate between pulsatile and sustained signals in the regulation of downstream gene expression.While networks underlying this signal decoding are diverse,many are built around a common motif, the incoherent feedforward loop (IFFL),where an input simultaneously activates an output and an inhibitor of the output.With appropriate parameters,this motif can generate temporal adaptation,where the system is desensitized to a sustained input.This property serves as the foundation for distinguishing signals with varying temporal profiles.Here,we use quantitative modeling to examine another property of IFFLs,the ability to process oscillatory signals.Our results indicate that the system's ability to translate pulsatile dynamics is limited by two constraints.The kinetics of IFFL components dictate the input range for which the network can decode pulsatile dynamics.In addition,a match between the network parameters and signal characteristics is required for optimal ``counting''.We elucidate one potential mechanism by which information processing occurs in natural networks with implications in the design of synthetic gene circuits for this purpose. This work was partially supported by the National Science Foundation Graduate Research Fellowship (CZ).

  8. ADM-Aeolus: wind profiles from space

    Science.gov (United States)

    de Kloe, Jos; Stoffelen, Ad; Marseille, Gert-Jan

    2017-04-01

    ADM-Aeolus is primarily a research and demonstration mission, flying the first Doppler wind lidar in space, scheduled to be launched end of 2017 by ESA. The lidar is operated in the UV at 355 nm wavelength. Part of the emitted signal is scattered back by molecules (Rayleigh scattering) and detected by the instrument Rayleigh channel. Another part of the emitted signal is scattered back by aerosol and cloud particles (Mie scattering), and detected by the Mie channel. The light received is Doppler shifted due to the movement of the scatterers along the laser Line- Of- Sight (LOS) and a horizontal LOS (HLOS) wind component profile is infered which extends from the surface up to a height of about 30 km. The number of rangebins is limited to 24 per channel. The vertical resolution is adjustable and will typically be 250 m close to the surface, 1 km in the free troposphere and 2 km in the stratosphere. The horizontal resolution is adjustable as well, with a raw resolution of around 3 km, but signals will be accumulated into observations of typically 84 km length to improve the signal-to-noise ratio before processing. ADM-Aeolus aims to measure wind component profiles with a quality comparable to radiosonde soundings to expect substantial impact on NWP models. To achieve this, signals measured in clear air will be separated from signals measured in scenes containing clouds or aerosols, before accumulation over 84 km. This requires a feature detection algorithm, which is part of the level-2B (L2B) processing, and based on detection of cross-talk, i.e. detection of Mie signal in the Rayleigh channel signal. This allows the flexibility to position the Rayleigh range bins in the stratosphere, without the need of a co-located Mie range bin. Cross-talk detection is based on comparing the measured and expected signal. The latter is calculated from auxiliary meteorological information obtained from NWP input, typically temperature and pressure, and instrument calibration

  9. Signaling in the Rhizosphere.

    Science.gov (United States)

    Venturi, Vittorio; Keel, Christoph

    2016-03-01

    Signaling studies in the rhizosphere have focused on close interactions between plants and symbiotic microorganisms. However, this focus is likely to expand to other microorganisms because the rhizomicrobiome is important for plant health and is able to influence the structure of the microbial community. We discuss here the shaping of the rhizomicrobiome and define which aspects can be considered signaling. We divide signaling in the rhizosphere into three categories: (i) between microbes, (ii) from plants to microorganisms, and (iii) from microorganisms to plants. Signals act on diverse organisms including the plant. Mycorrhizal and rhizobial interkingdom signaling has revealed its pivotal role in establishing associations, and the recent discovery of signaling with non-symbiotic microorganisms indicates the important role of communication in shaping the rhizomicrobiome. Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. Bicoid Signal Extraction with a Selection of Parametric and Nonparametric Signal Processing Techniques

    Institute of Scientific and Technical Information of China (English)

    Zara Ghodsi; Emmanuel Sirimal Silva; Hossein Hassani

    2015-01-01

    The maternal segmentation coordinate gene bicoid plays a significant role during Drosophila embryogenesis. The gradient of Bicoid, the protein encoded by this gene, determines most aspects of head and thorax development. This paper seeks to explore the applicability of a variety of signal processing techniques at extracting bicoid expression signal, and whether these methods can outperform the current model. We evaluate the use of six different powerful and widely-used models representing both parametric and nonparametric signal processing techniques to determine the most efficient method for signal extraction in bicoid. The results are evaluated using both real and simulated data. Our findings show that the Singular Spectrum Analysis technique proposed in this paper outperforms the synthesis diffusion degradation model for filtering the noisy protein profile of bicoid whilst the exponential smoothing technique was found to be the next best alternative followed by the autoregressive integrated moving average.

  11. Bicoid signal extraction with a selection of parametric and nonparametric signal processing techniques.

    Science.gov (United States)

    Ghodsi, Zara; Silva, Emmanuel Sirimal; Hassani, Hossein

    2015-06-01

    The maternal segmentation coordinate gene bicoid plays a significant role during Drosophila embryogenesis. The gradient of Bicoid, the protein encoded by this gene, determines most aspects of head and thorax development. This paper seeks to explore the applicability of a variety of signal processing techniques at extracting bicoid expression signal, and whether these methods can outperform the current model. We evaluate the use of six different powerful and widely-used models representing both parametric and nonparametric signal processing techniques to determine the most efficient method for signal extraction in bicoid. The results are evaluated using both real and simulated data. Our findings show that the Singular Spectrum Analysis technique proposed in this paper outperforms the synthesis diffusion degradation model for filtering the noisy protein profile of bicoid whilst the exponential smoothing technique was found to be the next best alternative followed by the autoregressive integrated moving average.

  12. Country Profiles, Pakistan.

    Science.gov (United States)

    Hardee, J. Gilbert; Satterthwaite, Adaline P.

    A profile of Pakistan is sketched in this paper. Emphasis is placed on the nature, scope, and accomplishments of population activities in the country. Topics and sub-topics include: location and description of the country; population (size, growth patterns, age structure, urban/rural distribution, ethnic and religious composition, migration,…

  13. Sensing the wind profile

    DEFF Research Database (Denmark)

    Pena Diaz, Alfredo

    prole. The boundary-layer height is derived in nearneutral and stable conditions based on turbulent momentum uxes only and in unstable conditions based on profiles of aerosol backscatter from ceilometer measurements. The lidar measuring technique is used to estimate momentum flux, showing high agreement...

  14. A temperature profiler

    Digital Repository Service at National Institute of Oceanography (India)

    Peshwe, V.B.; Desa, E.

    An instrument developed for measuring temperature profiles at sea in depth or time scales is described. PC-based programming offers flexibility in setting up the instrument for the mode of operation prior to each cast. A real time clock built...

  15. A Danish Profiling System

    DEFF Research Database (Denmark)

    Rosholm, Michael; Staghøj, Jonas; Svarer, Michael;

    2006-01-01

    This paper describes the statistical model used for profiling new unemployed workers in Denmark. When a worker – during his or her first six months in unemployment – enters the employment office for the first time, this model predicts whether or not he or she will be unemployed for more than six ...

  16. Country Education Profiles: Algeria.

    Science.gov (United States)

    International Bureau of Education, Geneva (Switzerland).

    One of a series of profiles prepared by the Cooperative Educational Abstracting Service, this brief outline provides basic background information on educational principles, system of administration, structure and organization, curricula, and teacher training in Algeria. Statistics provided by the Unesco Office of Statistics show enrollment at all…

  17. Country Profiles, Malaysia.

    Science.gov (United States)

    Marzuki, Ariffin Bin; Peng, J. Y.

    A profile of Malaysia is sketched in this paper. Emphasis is placed on the nature, scope, and accomplishments of population activities in the country. Topics and sub-topics include: location and description of the country; population (size, growth patterns, age structure, urban/rural distribution, ethnic and religious composition, migration,…

  18. English Teaching Profile: Mexico.

    Science.gov (United States)

    British Council, London (England). English Language and Literature Div.

    This profile of the English language teaching situation in Mexico examines the role of English in society and in the educational system. It is noted that the extent to which English is used in Mexico is affected by the country's proximity to the United States. The educational system is described, with emphasis on English instruction which begins…

  19. Geographic profiling survey

    NARCIS (Netherlands)

    Emeno, Karla; Bennell, Craig; Snook, Brent; Taylor, Paul Jonathon

    Geographic profiling (GP) is an investigative technique that involves predicting a serial offender?s home location (or some other anchor point) based on where he or she committed a crime. Although the use of GP in police investigations appears to be on the rise, little is known about the procedure

  20. COMPENDEX Profile Adjustment Manual.

    Science.gov (United States)

    Standera, Oldrich

    If an information system is to survive, the users must be satisfied that it meets their needs promptly and consistently. It is essential to react quickly to any undesired result such as an extemely high or low output, too low a relevance or recall, or both. The search editor should feel responsbile not only for the profile setup but also for its…

  1. The Intensity Profile of the Solar Supergranulation

    CERN Document Server

    Goldbaum, N J; Ermolli, I; Sands, J S; Berrilli, F

    2009-01-01

    We have measured the average radial (cell center to network boundary) profile of the continuum intensity contrast associated with supergranular flows using data from the Precision Solar Photometric Telescope (PSPT) at the Mauna Loa Solar Observatory (MLSO). After removing the contribution of the network flux elements by the application of masks based on Ca II K intensity and averaging over more than 10^5 supergranular cells, we find a ~ 0.1% decrease in red and blue continuum intensity from the supergranular cell centers outward, corresponding to a ~ 1.0 K decrease in brightness temperature across the cells. The radial intensity profile may be caused either by the thermal signal associated with the supergranular flows or a variation in the packing density of unresolved magnetic flux elements. These are not unambiguously distinguished by the observations, and we raise the possibility that the network magnetic fields play an active role in supergranular scale selection by enhancing the radiative cooling of the ...

  2. Biomedical signals and systems

    CERN Document Server

    Tranquillo, Joseph V

    2013-01-01

    Biomedical Signals and Systems is meant to accompany a one-semester undergraduate signals and systems course. It may also serve as a quick-start for graduate students or faculty interested in how signals and systems techniques can be applied to living systems. The biological nature of the examples allows for systems thinking to be applied to electrical, mechanical, fluid, chemical, thermal and even optical systems. Each chapter focuses on a topic from classic signals and systems theory: System block diagrams, mathematical models, transforms, stability, feedback, system response, control, time

  3. Digital signal processing

    CERN Document Server

    O'Shea, Peter; Hussain, Zahir M

    2011-01-01

    In three parts, this book contributes to the advancement of engineering education and that serves as a general reference on digital signal processing. Part I presents the basics of analog and digital signals and systems in the time and frequency domain. It covers the core topics: convolution, transforms, filters, and random signal analysis. It also treats important applications including signal detection in noise, radar range estimation for airborne targets, binary communication systems, channel estimation, banking and financial applications, and audio effects production. Part II considers sel

  4. Geolocation of RF signals

    CERN Document Server

    Progri, Ilir

    2011-01-01

    ""Geolocation of RF Signals - Principles and Simulations"" offers an overview of the best practices and innovative techniques in the art and science of geolocation over the last twenty years. It covers all research and development aspects including theoretical analysis, RF signals, geolocation techniques, key block diagrams, and practical principle simulation examples in the frequency band from 100 MHz to 18 GHz or even 60 GHz. Starting with RF signals, the book progressively examines various signal bands - such as VLF, LF, MF, HF, VHF, UHF, L, S, C, X, Ku, and, K and the corresponding geoloca

  5. Slit-Robo signaling.

    Science.gov (United States)

    Blockus, Heike; Chédotal, Alain

    2016-09-01

    Slits are secreted proteins that bind to Roundabout (Robo) receptors. Slit-Robo signaling is best known for mediating axon repulsion in the developing nervous system. However, in recent years the functional repertoire of Slits and Robo has expanded tremendously and Slit-Robo signaling has been linked to roles in neurogenesis, angiogenesis and cancer progression among other processes. Likewise, our mechanistic understanding of Slit-Robo signaling has progressed enormously. Here, we summarize new insights into Slit-Robo evolutionary and system-dependent diversity, receptor-ligand interactions, signaling crosstalk and receptor activation.

  6. Neutrino signals from dark matter

    Science.gov (United States)

    Erkoca, Arif Emre

    Large-scale neutrino telescopes will be powerful tools to observe multitude of mysterious phenomena happening in the Universe. The dark matter puzzle is listed as one of them. In this study, indirect detection of dark matter via neutrino signals is presented. The upward muon, the contained muon and the hadronic shower fluxes are calculated, assuming annihilation/decay of the dark matter in the core of the astrophysical objects and in the Galactic center. Direct neutrino production and secondary neutrino production from the decay of Standard Model particles produced in the annihilation/decay of dark matter are studied. The results are contrasted to the ones previously obtained in the literature, illustrating the importance of properly treating muon propagation and energy loss for the upward muon flux. The dependence of the dark matter signals on the density profile, the dark matter mass and the detector threshold are discussed. Different dark matter models (gravitino, Kaluza-Klein and leptophilic) which can account for recent observations of some indirect searches are analyzed regarding their detection in the kilometer size neutrino detectors in the near future. Muon and shower rates and the minimum observation times in order to reach 2sigma detection significance are evaluated, with the result suggesting that the optimum cone half angles chosen about the Galactic center are about 10° (50°) for the muon (shower) events. A detailed analysis shows that for the annihilating dark matter models such as the leptophilic and Kaluza-Klein models, upward and contained muon as well as showers yield promising signals for dark matter detection in just a few years of observation, whereas for decaying dark matter models, the same observation times can only be reached with showers. The analytical results for the final fluxes are also obtained as well as parametric forms for the muon and shower fluxes for the dark matter models considered in this study.

  7. Localization profile of Cathepsin L in the brain of African giant rat ...

    African Journals Online (AJOL)

    Localization profile of Cathepsin L in the brain of African giant rat ( Cricestomys gambianus ) ... Log in or Register to get access to full text downloads. ... Within the diencephalon high density of positive signals was observed in mediodorsal and ...

  8. Experimental investigation on wake profile detection based on laser scattering by bubbles

    Institute of Scientific and Technical Information of China (English)

    Liping Su; Weijiang Zhao; Xiaoyong Hu; Deming Ren; Xizhan Liu

    2007-01-01

    @@ The optical system for detecting wake profiles based on laser backscattering by bubbles at 180° is reported, in which the monostatic optical geometry is adopted and the power density estimation is used to process bubble scattering signal.

  9. Insulin resistance, adipokine profile and hepatic expression of SOCS-3 gene in chronic hepatitis C

    OpenAIRE

    Wójcik, Kamila; Jabłonowska, Elżbieta; Omulecka, Aleksandra; Piekarska, Anna

    2014-01-01

    AIM: To analyze adipokine concentrations, insulin resistance and hepatic expression of suppressor of cytokine signaling 3 (SOCS-3) in patients with chronic hepatitis C genotype 1 with normal body weight, glucose and lipid profile.

  10. Flux profile scanners for scattered high-energy electrons

    Science.gov (United States)

    Hicks, R. S.; Decowski, P.; Arroyo, C.; Breuer, M.; Celli, J.; Chudakov, E.; Kumar, K. S.; Olson, M.; Peterson, G. A.; Pope, K.; Ricci, J.; Savage, J.; Souder, P. A.

    2005-11-01

    The paper describes the design and performance of flux integrating Cherenkov scanners with air-core reflecting light guides used in a high-energy, high-flux electron scattering experiment at the Stanford Linear Accelerator Center. The scanners were highly radiation resistant and provided a good signal to background ratio leading to very good spatial resolution of the scattered electron flux profile scans.

  11. Fetal Biophysical Profile Scoring

    Directory of Open Access Journals (Sweden)

    H.R. HaghighatKhah

    2009-01-01

    Full Text Available   "nFetal biophysical profile scoring is a sonographic-based method of fetal assessment first described by Manning and Platt in 1980. "nThe biophysical profile score was developed as a method to integrate real-time observations of the fetus and his/her intrauterine environment in order to more comprehensively assess the fetal condition. These findings must be evaluated in the context of maternal/fetal history (i.e., chronic hypertension, post-dates, intrauterine growth restriction, etc, fetal structural integrity (presence or absence of congenital anomalies, and the functionality of fetal support structures (placental and umbilical cord. For example, acute asphyxia due to placental abruption may result in an absence of the acute variables of the biophysical profile score (fetal breathing movements, fetal movement, fetal tone, and fetal heart rate reactivity with a normal amniotic fluid volume. With post maturity the asphyxial event may be intermittent and chronic resulting in a decrease in amniotic fluid volume, but with the acute variables remaining normal. "nWhile the 5 components of the biophysical profile score have remained unchanged since 1980 (Manning, 1980, the definitions of a normal and abnormal parameter have evolved with increasing experience. "nIn 1984 the definition of oligohydramnios was increased from < 1cm pocket of fluid to < 2.0 x 1.0 cm pocket. Oligohydramnios is now defined as a pocket of amniotic fluid < 2.0 x 2.0 cm (Manning, 1995a "nIf the four ultrasound variables are normal, the accuracy of the biophysical profile score was not found to be significantly improved by adding the non-stress test. As a result, in 1987 the profile score was modified to incorporate the non-stress test only when one of the ultrasound variables was abnormal (Manning 1987. Table 1 outlines the current definitions for quantifying a variable as present or absent. "nEach of the 5 components of the biophysical profile score does not have equal

  12. Dictionary learning of sound speed profiles

    CERN Document Server

    Bianco, Michael

    2016-01-01

    To provide constraints on their inversion, ocean sound speed profiles (SSPs) often are modeled using empirical orthogonal functions (EOFs). However, this regularization, which uses the leading order EOFs with a minimum-energy constraint on their coefficients, often yields low resolution SSP estimates. In this paper, it is shown that dictionary learning, a form of unsupervised machine learning, can improve SSP resolution by generating a dictionary of shape functions for sparse processing (e.g. compressive sensing) that optimally compress SSPs; both minimizing the reconstruction error and the number of coefficients. These learned dictionaries (LDs) are not constrained to be orthogonal and thus, fit the given signals such that each signal example is approximated using few LD entries. Here, LDs describing SSP observations from the HF-97 experiment and the South China Sea are generated using the K-SVD algorithm. These LDs better explain SSP variability and require fewer coefficients than EOFs, describing much of t...

  13. Signal sampling circuit

    NARCIS (Netherlands)

    Louwsma, S.M.; Vertregt, Maarten

    2010-01-01

    A sampling circuit for sampling a signal is disclosed. The sampling circuit comprises a plurality of sampling channels adapted to sample the signal in time-multiplexed fashion, each sampling channel comprising a respective track-and-hold circuit connected to a respective analogue to digital converte

  14. Signal sampling circuit

    NARCIS (Netherlands)

    Louwsma, S.M.; Vertregt, Maarten

    2011-01-01

    A sampling circuit for sampling a signal is disclosed. The sampling circuit comprises a plurality of sampling channels adapted to sample the signal in time-multiplexed fashion, each sampling channel comprising a respective track-and-hold circuit connected to a respective analogue to digital converte

  15. Signaling by Gasotransmitters

    OpenAIRE

    Mustafa, Asif K.; Gadalla, Moataz M.; Snyder, Solomon H

    2009-01-01

    Nitric oxide is well established as a major signaling molecule. Evidence is accumulating that carbon monoxide and hydrogen sulfide also are physiologic mediators in the cardiovascular, immune, and nervous systems. This Review focuses on mechanisms whereby they signal by binding to metal centers in metalloproteins, such as in guanylyl cyclase, or modifying sulfhydryl groups in protein targets.

  16. Ubiquitination in apoptosis signaling

    NARCIS (Netherlands)

    van de Kooij, L.W.

    2014-01-01

    The work described in this thesis focuses on ubiquitination and protein degradation, with an emphasis on how these processes regulate apoptosis signaling. More specifically, our aims were: 1. To increase the understanding of ubiquitin-mediated regulation of apoptosis signaling. 2. To identify the E3

  17. Multiresolution signal decomposition schemes

    NARCIS (Netherlands)

    J. Goutsias (John); H.J.A.M. Heijmans (Henk)

    1998-01-01

    textabstract[PNA-R9810] Interest in multiresolution techniques for signal processing and analysis is increasing steadily. An important instance of such a technique is the so-called pyramid decomposition scheme. This report proposes a general axiomatic pyramid decomposition scheme for signal analysis

  18. Multiresolution signal decomposition schemes

    NARCIS (Netherlands)

    Goutsias, J.; Heijmans, H.J.A.M.

    1998-01-01

    [PNA-R9810] Interest in multiresolution techniques for signal processing and analysis is increasing steadily. An important instance of such a technique is the so-called pyramid decomposition scheme. This report proposes a general axiomatic pyramid decomposition scheme for signal analysis and synthes

  19. SignalR blueprints

    CERN Document Server

    Ingebrigtsen, Einar

    2015-01-01

    This book is designed for software developers, primarily those with knowledge of C#, .NET, and JavaScript. Good knowledge and understanding of SignalR is assumed to allow efficient programming of core elements and applications in SignalR.

  20. MBA Quality Signals.

    Science.gov (United States)

    Chapman, Randall S.

    1998-01-01

    A study identified quality signals for master's programs in business administration (MBAs). Traditional scholarly oriented academic signals are apparently not valued as such by external customer groups. MBA academic quality appears to be a multidimensional construct, with subdimensions of real-worldness; placement; student satisfaction; and…

  1. Ubiquitination in apoptosis signaling

    NARCIS (Netherlands)

    van de Kooij, L.W.

    2014-01-01

    The work described in this thesis focuses on ubiquitination and protein degradation, with an emphasis on how these processes regulate apoptosis signaling. More specifically, our aims were: 1. To increase the understanding of ubiquitin-mediated regulation of apoptosis signaling. 2. To identify the E3

  2. Signaling in symbiosis

    NARCIS (Netherlands)

    Limpens, E.H.M.; Bisseling, T.

    2003-01-01

    In recent years, the major focus in nodulation research has been on the genetic dissection of Nod-factor signaling. Components of this pathway appear to be shared with signaling processes that are induced during the formation of mycorrhiza. With the cloning of orthologs of the NIN and DMI2 genes

  3. Signaling in symbiosis

    NARCIS (Netherlands)

    Limpens, E.H.M.; Bisseling, T.

    2003-01-01

    In recent years, the major focus in nodulation research has been on the genetic dissection of Nod-factor signaling. Components of this pathway appear to be shared with signaling processes that are induced during the formation of mycorrhiza. With the cloning of orthologs of the NIN and DMI2 genes fro

  4. Signal sampling circuit

    NARCIS (Netherlands)

    Louwsma, Simon Minze; Vertregt, Maarten

    2011-01-01

    A sampling circuit for sampling a signal is disclosed. The sampling circuit comprises a plurality of sampling channels adapted to sample the signal in time-multiplexed fashion, each sampling channel comprising a respective track-and-hold circuit connected to a respective analogue to digital converte

  5. Signal sampling circuit

    NARCIS (Netherlands)

    Louwsma, Simon Minze; Vertregt, Maarten

    2010-01-01

    A sampling circuit for sampling a signal is disclosed. The sampling circuit comprises a plurality of sampling channels adapted to sample the signal in time-multiplexed fashion, each sampling channel comprising a respective track-and-hold circuit connected to a respective analogue to digital converte

  6. RESIDUAL-GAS-IONIZATION BEAM PROFILE MONITORS IN RHIC.

    Energy Technology Data Exchange (ETDEWEB)

    CONNOLLY, R.; MICHNOFF, R.; TEPIKIAN, S.

    2005-05-16

    Four ionization profile monitors (IPMs) in RHIC measure vertical and horizontal beam profiles in the two rings by measuring the distribution of electrons produced by beam ionization of residual gas. During the last three years both the collection accuracy and signal/noise ratio have been improved. An electron source is mounted across the beam pipe from the collector to monitor microchannel plate (MCP) aging and the signal electrons are gated to reduce MCP aging and to allow charge replenishment between single-turn measurements. Software changes permit simultaneous measurements of any number of individual bunches in the ring. This has been used to measure emittance growth rates on six bunches of varying intensities in a single store. Also the software supports FFT analysis of turn-by-turn profiles of a single bunch at injection to detect dipole and quadrupole oscillations.

  7. Digital signaling decouples activation probability and population heterogeneity

    DEFF Research Database (Denmark)

    Kellogg, Ryan A; Tian, Chengzhe; Lipniacki, Tomasz;

    2015-01-01

    Digital signaling enhances robustness of cellular decisions in noisy environments, but it is unclear how digital systems transmit temporal information about a stimulus. To understand how temporal input information is encoded and decoded by the NF-κB system, we studied transcription factor dynamic...... and uniform dynamics. These results show that digital NF-κB signaling enables multidimensional control of cellular phenotype via input profile, allowing parallel and independent control of single-cell activation probability and population heterogeneity....

  8. Calcium signaling and epilepsy.

    Science.gov (United States)

    Steinlein, Ortrud K

    2014-08-01

    Calcium signaling is involved in a multitude of physiological and pathophysiological mechanisms. Over the last decade, it has been increasingly recognized as an important factor in epileptogenesis, and it is becoming obvious that the excess synchronization of neurons that is characteristic for seizures can be linked to various calcium signaling pathways. These include immediate effects on membrane excitability by calcium influx through ion channels as well as delayed mechanisms that act through G-protein coupled pathways. Calcium signaling is able to cause hyperexcitability either by direct modulation of neuronal activity or indirectly through calcium-dependent gliotransmission. Furthermore, feedback mechanisms between mitochondrial calcium signaling and reactive oxygen species are able to cause neuronal cell death and seizures. Unravelling the complexity of calcium signaling in epileptogenesis is a daunting task, but it includes the promise to uncover formerly unknown targets for the development of new antiepileptic drugs.

  9. Molecular and Cellular Signaling

    CERN Document Server

    Beckerman, Martin

    2005-01-01

    A small number of signaling pathways, no more than a dozen or so, form a control layer that is responsible for all signaling in and between cells of the human body. The signaling proteins belonging to the control layer determine what kinds of cells are made during development and how they function during adult life. Malfunctions in the proteins belonging to the control layer are responsible for a host of human diseases ranging from neurological disorders to cancers. Most drugs target components in the control layer, and difficulties in drug design are intimately related to the architecture of the control layer. Molecular and Cellular Signaling provides an introduction to molecular and cellular signaling in biological systems with an emphasis on the underlying physical principles. The text is aimed at upper-level undergraduates, graduate students and individuals in medicine and pharmacology interested in broadening their understanding of how cells regulate and coordinate their core activities and how diseases ...

  10. Neutron signal transfer analysis

    CERN Document Server

    Pleinert, H; Lehmann, E

    1999-01-01

    A new method called neutron signal transfer analysis has been developed for quantitative determination of hydrogenous distributions from neutron radiographic measurements. The technique is based on a model which describes the detector signal obtained in the measurement as a result of the action of three different mechanisms expressed by signal transfer functions. The explicit forms of the signal transfer functions are determined by Monte Carlo computer simulations and contain only the distribution as a variable. Therefore an unknown distribution can be determined from the detector signal by recursive iteration. This technique provides a simple and efficient tool for analysis of this type while also taking into account complex effects due to the energy dependency of neutron interaction and single and multiple scattering. Therefore this method provides an efficient tool for precise quantitative analysis using neutron radiography, as for example quantitative determination of moisture distributions in porous buil...

  11. Tunable Signal Processing through a Kinase Control Cycle: the IKK Signaling Node

    Science.gov (United States)

    Behar, Marcelo; Hoffmann, Alexander

    2013-01-01

    The transcription factor NFκB, a key component of the immune system, shows intricate stimulus-specific temporal dynamics. Those dynamics are thought to play a role in controlling the physiological response to cytokines and pathogens. Biochemical evidence suggests that the NFκB inducing kinase, IKK, a signaling hub onto which many signaling pathways converge, is regulated via a regulatory cycle comprising a poised, an active, and an inactive state. We hypothesize that it operates as a modulator of signal dynamics, actively reshaping the signals generated at the receptor proximal level. Here we show that a regulatory cycle can function in at least three dynamical regimes, tunable by regulating a single kinetic parameter. In particular, the simplest three-state regulatory cycle can generate signals with two well-defined phases, each with distinct coding capabilities in terms of the information they can carry about the stimulus. We also demonstrate that such a kinase cycle can function as a signal categorizer classifying diverse incoming signals into outputs with a limited set of temporal activity profiles. Finally, we discuss the extension of the results to other regulatory motifs that could be understood in terms of the regimes of the three-state cycle. PMID:23823243

  12. Profile of success

    DEFF Research Database (Denmark)

    Dahlgaard, Jens Jørn; Nørgaard, Anders; Jakobsen, Søren

    1998-01-01

    What management skills must Europe's business leaders improve to achieve business excellence? Which country's leaders are best placed for success? Does the next generation have what it takes to compete? In the second half of their study of the leadership styles that drive business excellence, Jens...... Dahlgaard, Anders Nørgaard and Søren Jakobsen describe an excellent leadership profile that provides the answers....

  13. Qualitative Value Profiling

    DEFF Research Database (Denmark)

    Duus, Henrik Johannsen; Bjerre, Mogens

    2015-01-01

    Qualitative value profiling (QVP) is a relatively unknown method of strategic analysis for companies in international business-to-business settings. The purpose of QVP is to reduce the information complexity that is faced by international companies in dealing with business partners. The QVP method...... and consequently, for the advancement of international development. Further use of QVP is recommended and suggestions for future research are provided....

  14. High throughput drug profiling

    OpenAIRE

    Entzeroth, Michael; Chapelain, Béatrice; Guilbert, Jacques; Hamon, Valérie

    2000-01-01

    High throughput screening has significantly contributed to advances in drug discovery. The great increase in the number of samples screened has been accompanied by increases in costs and in the data required for the investigated compounds. High throughput profiling addresses the issues of compound selectivity and specificity. It combines conventional screening with data mining technologies to give a full set of data, enabling development candidates to be more fully compared.

  15. Practical Differential Profiling

    Energy Technology Data Exchange (ETDEWEB)

    Schulz, M; De Supinski, B R

    2007-02-04

    Comparing performance profiles from two runs is an essential performance analysis step that users routinely perform. In this work we present eGprof, a tool that facilitates these comparisons through differential profiling inside gprof. We chose this approach, rather than designing a new tool, since gprof is one of the few performance analysis tools accepted and used by a large community of users. eGprof allows users to 'subtract' two performance profiles directly. It also includes callgraph visualization to highlight the differences in graphical form. Along with the design of this tool, we present several case studies that show how eGprof can be used to find and to study the differences of two application executions quickly and hence can aid the user in this most common step in performance analysis. We do this without requiring major changes on the side of the user, the most important factor in guaranteeing the adoption of our tool by code teams.

  16. Wide HI profile galaxies

    CERN Document Server

    Brosch, Noah; Zitrin, Adi

    2011-01-01

    We investigate the nature of objects in a complete sample of 28 galaxies selected from the first sky area fully covered by ALFALFA, being well-detected and having HI profiles wider than 550 km/s. The selection does not use brightness, morphology, or any other property derived from optical or other spectral bands. We investigate the degree of isolation, the morphology, and other properties gathered or derived from open data bases and show that some objects have wide HI profiles probably because they are disturbed or are interacting, or might be confused in the ALFALFA beam. We identify a sub-sample of 14 galaxies lacking immediate interacting neighbours and showing regular, symmetric, two-horned HI profiles that we propose as candidate high-mass disk systems (CHMDs). We measure the net-Halpha emission from the CHMDs and combine this with public multispectral data to model the global star formation (SF) properties of each galaxy. The Halpha observations show SFRs not higher than a few solar masses per year. Sim...

  17. A Quantitative Profiling Tool for Diverse Genomic Data Types Reveals Potential Associations between Chromatin and Pre-mRNA Processing.

    Science.gov (United States)

    Kremsky, Isaac; Bellora, Nicolás; Eyras, Eduardo

    2015-01-01

    High-throughput sequencing, and genome-based datasets in general, are often represented as profiles centered at reference points to study the association of protein binding and other signals to particular regulatory mechanisms. Although these profiles often provide compelling evidence of these associations, they do not provide a quantitative assessment of the enrichment, which makes the comparison between signals and conditions difficult. In addition, a number of biases can confound profiles, but are rarely accounted for in the tools currently available. We present a novel computational method, ProfileSeq, for the quantitative assessment of biological profiles to provide an exact, nonparametric test that specific regions of the test profile have higher or lower signal densities than a control set. The method is applicable to high-throughput sequencing data (ChIP-Seq, GRO-Seq, CLIP-Seq, etc.) and to genome-based datasets (motifs, etc.). We validate ProfileSeq by recovering and providing a quantitative assessment of several results reported before in the literature using independent datasets. We show that input signal and mappability have confounding effects on the profile results, but that normalizing the signal by input reads can eliminate these biases while preserving the biological signal. Moreover, we apply ProfileSeq to ChIP-Seq data for transcription factors, as well as for motif and CLIP-Seq data for splicing factors. In all examples considered, the profiles were robust to biases in mappability of sequencing reads. Furthermore, analyses performed with ProfileSeq reveal a number of putative relationships between transcription factor binding to DNA and splicing factor binding to pre-mRNA, adding to the growing body of evidence relating chromatin and pre-mRNA processing. ProfileSeq provides a robust way to quantify genome-wide coordinate-based signal. Software and documentation are freely available for academic use at https://bitbucket.org/regulatorygenomicsupf/profileseq/.

  18. A Quantitative Profiling Tool for Diverse Genomic Data Types Reveals Potential Associations between Chromatin and Pre-mRNA Processing.

    Directory of Open Access Journals (Sweden)

    Isaac Kremsky

    Full Text Available High-throughput sequencing, and genome-based datasets in general, are often represented as profiles centered at reference points to study the association of protein binding and other signals to particular regulatory mechanisms. Although these profiles often provide compelling evidence of these associations, they do not provide a quantitative assessment of the enrichment, which makes the comparison between signals and conditions difficult. In addition, a number of biases can confound profiles, but are rarely accounted for in the tools currently available. We present a novel computational method, ProfileSeq, for the quantitative assessment of biological profiles to provide an exact, nonparametric test that specific regions of the test profile have higher or lower signal densities than a control set. The method is applicable to high-throughput sequencing data (ChIP-Seq, GRO-Seq, CLIP-Seq, etc. and to genome-based datasets (motifs, etc.. We validate ProfileSeq by recovering and providing a quantitative assessment of several results reported before in the literature using independent datasets. We show that input signal and mappability have confounding effects on the profile results, but that normalizing the signal by input reads can eliminate these biases while preserving the biological signal. Moreover, we apply ProfileSeq to ChIP-Seq data for transcription factors, as well as for motif and CLIP-Seq data for splicing factors. In all examples considered, the profiles were robust to biases in mappability of sequencing reads. Furthermore, analyses performed with ProfileSeq reveal a number of putative relationships between transcription factor binding to DNA and splicing factor binding to pre-mRNA, adding to the growing body of evidence relating chromatin and pre-mRNA processing. ProfileSeq provides a robust way to quantify genome-wide coordinate-based signal. Software and documentation are freely available for academic use at https://bitbucket.org/regulatorygenomicsupf/profileseq/.

  19. Notch signaling in acute promyelocytic leukemia.

    Science.gov (United States)

    Grieselhuber, N R; Klco, J M; Verdoni, A M; Lamprecht, T; Sarkaria, S M; Wartman, L D; Ley, T J

    2013-07-01

    Acute promyelocytic leukemia (APL) is initiated by the PML-RARA (PR) fusion oncogene and has a characteristic expression profile that includes high levels of the Notch ligand Jagged-1 (JAG1). In this study, we used a series of bioinformatic, in vitro, and in vivo assays to assess the role of Notch signaling in human APL samples, and in a PML-RARA knock-in mouse model of APL (Ctsg-PML-RARA). We identified a Notch expression signature in both human primary APL cells and in Kit+Lin-Sca1+ cells from pre-leukemic Ctsg-PML-RARA mice. Both genetic and pharmacologic inhibition of Notch signaling abrogated the enhanced self-renewal seen in hematopoietic stem/progenitor cells from pre-leukemic Ctsg-PML-RARA mice, but had no influence on cells from age-matched wild-type mice. In addition, six of nine murine APL tumors tested displayed diminished growth in vitro when Notch signaling was inhibited pharmacologically. Finally, we found that genetic inhibition of Notch signaling with a dominant-negative Mastermind-like protein reduced APL growth in vivo in a subset of tumors. These findings expand the role of Notch signaling in hematopoietic diseases, and further define the mechanistic events important for PML-RARA-mediated leukemogenesis.

  20. Leflunomide : a manageable safety profile

    NARCIS (Netherlands)

    Riel, P.L.C.M. van; Smolen, J.S.; Emery, P.; Kalden, J.R.; Dougados, M.; Strand, C.V.; Breedveld, F.C.

    2004-01-01

    The safety profile of leflunomide in the treatment of rheumatoid arthritis has been well documented in clinical trials, postmarketing surveillance, and epidemiological studies. Both postmarketing surveillance and epidemiological study results are consistent with the safety profile of leflunomide rep

  1. Signal-dependent noise determines motor planning.

    Science.gov (United States)

    Harris, C M; Wolpert, D M

    1998-08-20

    When we make saccadic eye movements or goal-directed arm movements, there is an infinite number of possible trajectories that the eye or arm could take to reach the target. However, humans show highly stereotyped trajectories in which velocity profiles of both the eye and hand are smooth and symmetric for brief movements. Here we present a unifying theory of eye and arm movements based on the single physiological assumption that the neural control signals are corrupted by noise whose variance increases with the size of the control signal. We propose that in the presence of such signal-dependent noise, the shape of a trajectory is selected to minimize the variance of the final eye or arm position. This minimum-variance theory accurately predicts the trajectories of both saccades and arm movements and the speed-accuracy trade-off described by Fitt's law. These profiles are robust to changes in the dynamics of the eye or arm, as found empirically. Moreover, the relation between path curvature and hand velocity during drawing movements reproduces the empirical 'two-thirds power law. This theory provides a simple and powerful unifying perspective for both eye and arm movement control.

  2. Electrical signaling and photosynthesis

    Science.gov (United States)

    Mancuso, Stefano

    2011-01-01

    Mechanical irritation of trigger hairs and subsequent generation of action potentials have significant impact on photosynthesis and respiration in carnivorous Venus flytrap (Dionaea muscipula). Action potential-mediated inhibition of photosynthesis and stimulation of respiration is confined only to the trap and was not recorded in adjacent photosynthetic lamina. We showed that the main primary target of electrical signals on assimilation is in the dark enzymatic reaction of photosynthesis. Without doubt, the electrical signaling is costly, and the possible co-existence of such type of signals and photosynthesis in plant cell is discussed. PMID:21558815

  3. Signal flow analysis

    CERN Document Server

    Abrahams, J R; Hiller, N

    1965-01-01

    Signal Flow Analysis provides information pertinent to the fundamental aspects of signal flow analysis. This book discusses the basic theory of signal flow graphs and shows their relation to the usual algebraic equations.Organized into seven chapters, this book begins with an overview of properties of a flow graph. This text then demonstrates how flow graphs can be applied to a wide range of electrical circuits that do not involve amplification. Other chapters deal with the parameters as well as circuit applications of transistors. This book discusses as well the variety of circuits using ther

  4. Hanford Site Ecological Quality Profile

    Energy Technology Data Exchange (ETDEWEB)

    Bilyard, Gordon R.; Sackschewsky, Michael R.; Tzemos, Spyridon

    2002-02-17

    This report reviews the ecological quality profile methodology and results for the Hanford Site. It covers critical ecological assets and terrestrial resources, those in Columbia River corridor and those threatened and engdangered, as well as hazards and risks to terrestrial resources. The features of a base habitat value profile are explained, as are hazard and ecological quality profiles.

  5. Signal Station Inspection Reports

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Handwritten reports resulting from detailed inspections of US Army Signal Service Stations, 1871-1889. Features reported included instrument exposure and condition,...

  6. Lipid signaling in plants

    NARCIS (Netherlands)

    Munnik, T.

    2010-01-01

    This book highlights the current status of plant lipid signaling. Written by leading researchers in the field, the chapters include detailed information on the measurement, regulation and function of phospholipases, lipid kinases, lipid phosphatases, inositolpolyphosphates, polyphosphoinositides, ph

  7. Ultrahigh bandwidth signal processing

    DEFF Research Database (Denmark)

    Oxenløwe, Leif Katsuo

    2016-01-01

    Optical time lenses have proven to be very versatile for advanced optical signal processing. Based on a controlled interplay between dispersion and phase-modulation by e.g. four-wave mixing, the processing is phase-preserving, an hence useful for all types of data signals including coherent multi......-level modulation founats. This has enabled processing of phase-modulated spectrally efficient data signals, such as orthogonal frequency division multiplexed (OFDM) signa In that case, a spectral telescope system was used, using two time lenses with different focal lengths (chirp rates), yielding a spectral...... regeneratio These operations require a broad bandwidth nonlinear platform, and novel photonic integrated nonlinear platform like aluminum gallium arsenide nano-waveguides used for 1.28 Tbaud optical signal processing will be described....

  8. Transmembrane Signalling: Membrane messengers

    Science.gov (United States)

    Cockroft, Scott L.

    2017-05-01

    Life has evolved elaborate means of communicating essential chemical information across cell membranes. Inspired by biology, two new artificial mechanisms have now been developed that use synthetic messenger molecules to relay chemical signals into or across lipid membranes.

  9. Acoustic MIMO signal processing

    CERN Document Server

    Huang, Yiteng; Chen, Jingdong

    2006-01-01

    A timely and important book addressing a variety of acoustic signal processing problems under multiple-input multiple-output (MIMO) scenarios. It uniquely investigates these problems within a unified framework offering a novel and penetrating analysis.

  10. Signaling in muscle contraction.

    Science.gov (United States)

    Kuo, Ivana Y; Ehrlich, Barbara E

    2015-02-02

    Signaling pathways regulate contraction of striated (skeletal and cardiac) and smooth muscle. Although these are similar, there are striking differences in the pathways that can be attributed to the distinct functional roles of the different muscle types. Muscles contract in response to depolarization, activation of G-protein-coupled receptors and other stimuli. The actomyosin fibers responsible for contraction require an increase in the cytosolic levels of calcium, which signaling pathways induce by promoting influx from extracellular sources or release from intracellular stores. Rises in cytosolic calcium stimulate numerous downstream calcium-dependent signaling pathways, which can also regulate contraction. Alterations to the signaling pathways that initiate and sustain contraction and relaxation occur as a consequence of exercise and pathophysiological conditions.

  11. Signals from the Cosmos.

    Science.gov (United States)

    Lichtman, Jeffrey M.

    1991-01-01

    Introduces the basics of radio astronomy and describes how to assemble several simple systems for receiving radio signals from the cosmos. Includes schematics, parts lists, working drawings, and contact information for radio astronomy suppliers. (11 references) (Author/JJK)

  12. Plant lipid signaling protocols

    NARCIS (Netherlands)

    Munnik, T.; Heilmann, I.

    2013-01-01

    Eukaryotic cells are surrounded by membranes consisting of various lipids, including sterols, sphingolipids, glycolipids, and phospholipids. Besides structural functions, membranes also contain lipids with regulatory and signaling roles. Such lipids include polyphosphoinositides, the low-abundant

  13. Topological signal processing

    CERN Document Server

    Robinson, Michael

    2014-01-01

    Signal processing is the discipline of extracting information from collections of measurements. To be effective, the measurements must be organized and then filtered, detected, or transformed to expose the desired information.  Distortions caused by uncertainty, noise, and clutter degrade the performance of practical signal processing systems. In aggressively uncertain situations, the full truth about an underlying signal cannot be known.  This book develops the theory and practice of signal processing systems for these situations that extract useful, qualitative information using the mathematics of topology -- the study of spaces under continuous transformations.  Since the collection of continuous transformations is large and varied, tools which are topologically-motivated are automatically insensitive to substantial distortion. The target audience comprises practitioners as well as researchers, but the book may also be beneficial for graduate students.

  14. Digital signal processing: Handbook

    Science.gov (United States)

    Goldenberg, L. M.; Matiushkin, B. D.; Poliak, M. N.

    The fundamentals of the theory and design of systems and devices for the digital processing of signals are presented. Particular attention is given to algorithmic methods of synthesis and digital processing equipment in communication systems (e.g., selective digital filtering, spectral analysis, and variation of the signal discretization frequency). Programs for the computer-aided analysis of digital filters are described. Computational examples are presented, along with tables of transfer function coefficients for recursive and nonrecursive digital filters.

  15. GROUP PROFILE Computer Technique

    Directory of Open Access Journals (Sweden)

    Andrey V. Sidorenkov

    2015-01-01

    Full Text Available This article contains a description of the structure, the software and functional capabilities, and the scope and purposes of application of the Group Profile (GP computer technique. This technique rests on a conceptual basis (the microgroup theory, includes 16 new and modified questionnaires, and a unique algorithm, tied to the questionnaires, for identification of informal groups. The GP yields a wide range of data about the group as a whole (47 indices, each informal group (43 indices, and each group member (16 indices. The GP technique can be used to study different types of groups: production (work groups, design teams, military units, etc., academic (school classes, student groups, and sports.

  16. Cohort Profile Update

    DEFF Research Database (Denmark)

    Omland, Lars Haukali; Ahlström, Magnus Glindvad; Obel, Niels

    2014-01-01

    The DHCS is a cohort of all HIV-infected individuals seen in one of the eight Danish HIV centres after 31 December 1994. Here we update the 2009 cohort profile emphasizing the development of the cohort. Every 12-24 months, DHCS is linked with the Danish Civil Registration System (CRS) in order...... of Causes of Death, the Danish National Prescription Registry, the Attainment Register and the Integrated Database for Labour Market Research to get information on vital status, migration, cancer, hospital contacts, causes of death, dispensed prescriptions, education and employment. Using this design, rates...

  17. Signaling Over Distances.

    Science.gov (United States)

    Saito, Atsushi; Cavalli, Valeria

    2016-02-01

    Neurons are extremely polarized cells. Axon lengths often exceed the dimension of the neuronal cell body by several orders of magnitude. These extreme axonal lengths imply that neurons have mastered efficient mechanisms for long distance signaling between soma and synaptic terminal. These elaborate mechanisms are required for neuronal development and maintenance of the nervous system. Neurons can fine-tune long distance signaling through calcium wave propagation and bidirectional transport of proteins, vesicles, and mRNAs along microtubules. The signal transmission over extreme lengths also ensures that information about axon injury is communicated to the soma and allows for repair mechanisms to be engaged. This review focuses on the different mechanisms employed by neurons to signal over long axonal distances and how signals are interpreted in the soma, with an emphasis on proteomic studies. We also discuss how proteomic approaches could help further deciphering the signaling mechanisms operating over long distance in axons. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  18. Ultrahigh bandwidth signal processing

    Science.gov (United States)

    Oxenløwe, Leif Katsuo

    2016-04-01

    Optical time lenses have proven to be very versatile for advanced optical signal processing. Based on a controlled interplay between dispersion and phase-modulation by e.g. four-wave mixing, the processing is phase-preserving, and hence useful for all types of data signals including coherent multi-level modulation formats. This has enabled processing of phase-modulated spectrally efficient data signals, such as orthogonal frequency division multiplexed (OFDM) signals. In that case, a spectral telescope system was used, using two time lenses with different focal lengths (chirp rates), yielding a spectral magnification of the OFDM signal. Utilising such telescopic arrangements, it has become possible to perform a number of interesting functionalities, which will be described in the presentation. This includes conversion from OFDM to Nyquist WDM, compression of WDM channels to a single Nyquist channel and WDM regeneration. These operations require a broad bandwidth nonlinear platform, and novel photonic integrated nonlinear platforms like aluminum gallium arsenide nano-waveguides used for 1.28 Tbaud optical signal processing will be described.

  19. Technological requirements of profile machining

    Institute of Scientific and Technical Information of China (English)

    PARK Sangchul; CHUNG Yunchan

    2006-01-01

    The term ‘profile machining’is used to refer to the milling of vertical surfaces described by profile curves. Profile machining requires higher precision (1/1000 mm) than regular 3D machining (1/100 mm) with the erosion of sharp vertices should being especially avoided. Although, profile machining is very essential for making trimming and flangedies, it seldom brought into focus. This paper addresses the technological requirements of profile machining including machining width and depth control,minimizing toolware, and protecting sharp vertices. Issues of controller alarms are also addressed.

  20. Digital Signal Processing applied to Physical Signals

    CERN Document Server

    Alberto, Diego; Musa, L

    2011-01-01

    It is well known that many of the scientific and technological discoveries of the XXI century will depend on the capability of processing and understanding a huge quantity of data. With the advent of the digital era, a fully digital and automated treatment can be designed and performed. From data mining to data compression, from signal elaboration to noise reduction, a processing is essential to manage and enhance features of interest after every data acquisition (DAQ) session. In the near future, science will go towards interdisciplinary research. In this work there will be given an example of the application of signal processing to different fields of Physics from nuclear particle detectors to biomedical examinations. In Chapter 1 a brief description of the collaborations that allowed this thesis is given, together with a list of the publications co-produced by the author in these three years. The most important notations, definitions and acronyms used in the work are also provided. In Chapter 2, the last r...

  1. Sinusoidal phase-modulating laser diode interferometer for real-time surface profile measurement

    Institute of Scientific and Technical Information of China (English)

    Guotian He; Xiangzhao Wang; Aijun Zeng; Feng Tang

    2007-01-01

    A sinusoidal phase-modulating (SPM) laser diode (LD) interferometer for real-time surface profile measurement is proposed and its principle is analyzed. The phase signal of the surface profile is detected from the sinusoidal phase-modulating interference signal using a real-time phase detection circuit. For 60 × 60 measurement points of the surface profile, the measuring time is 10 ms. A root mean square (RMS) measurement repeatability of 3.93 nm is realized, and the measurement resolution reaches 0.19 nm.

  2. Deflagration Wave Profiles

    Energy Technology Data Exchange (ETDEWEB)

    Menikoff, Ralph [Los Alamos National Laboratory

    2012-04-03

    Shock initiation in a plastic-bonded explosives (PBX) is due to hot spots. Current reactive burn models are based, at least heuristically, on the ignition and growth concept. The ignition phase occurs when a small localized region of high temperature (or hot spot) burns on a fast time scale. This is followed by a growth phase in which a reactive front spreads out from the hot spot. Propagating reactive fronts are deflagration waves. A key question is the deflagration speed in a PBX compressed and heated by a shock wave that generated the hot spot. Here, the ODEs for a steady deflagration wave profile in a compressible fluid are derived, along with the needed thermodynamic quantities of realistic equations of state corresponding to the reactants and products of a PBX. The properties of the wave profile equations are analyzed and an algorithm is derived for computing the deflagration speed. As an illustrative example, the algorithm is applied to compute the deflagration speed in shock compressed PBX 9501 as a function of shock pressure. The calculated deflagration speed, even at the CJ pressure, is low compared to the detonation speed. The implication of this are briefly discussed.

  3. Glutamate signalling in bone.

    Directory of Open Access Journals (Sweden)

    Karen eBrakspear

    2012-08-01

    Full Text Available Mechanical loading plays a key role in the physiology of bone, allowing bone to functionally adapt to its environment, however characterisation of the signalling events linking load to bone formation is incomplete. A screen for genes associated with mechanical load-induced bone formation identified the glutamate transporter GLAST, implicating the excitatory amino acid, glutamate, in the mechanoresponse. When an osteogenic load (10N, 10Hz was externally applied to the rat ulna, GLAST (EAAT1 mRNA, was significantly down-regulated in osteocytes in the loaded limb. Functional components from each stage of the glutamate signalling pathway have since been identified within bone, including proteins necessary for calcium-mediated glutamate exocytosis, receptors, transporters and signal propagation. Activation of ionotropic glutamate receptors has been shown to regulate the phenotype of osteoblasts and osteoclasts in vitro and bone mass in vivo. Furthermore, glutamatergic nerves have been identified in the vicinity of bone cells expressing glutamate receptors in vivo. However, it is not yet known how a glutamate signalling event is initiated in bone or its physiological significance. This review will examine the role of the glutamate signalling pathway in bone, with emphasis on the functions of glutamate transporters in osteoblasts.

  4. VLSI signal processing technology

    CERN Document Server

    Swartzlander, Earl

    1994-01-01

    This book is the first in a set of forthcoming books focussed on state-of-the-art development in the VLSI Signal Processing area. It is a response to the tremendous research activities taking place in that field. These activities have been driven by two factors: the dramatic increase in demand for high speed signal processing, especially in consumer elec­ tronics, and the evolving microelectronic technologies. The available technology has always been one of the main factors in determining al­ gorithms, architectures, and design strategies to be followed. With every new technology, signal processing systems go through many changes in concepts, design methods, and implementation. The goal of this book is to introduce the reader to the main features of VLSI Signal Processing and the ongoing developments in this area. The focus of this book is on: • Current developments in Digital Signal Processing (DSP) pro­ cessors and architectures - several examples and case studies of existing DSP chips are discussed in...

  5. Genomic and metabolomic profile associated to microalbuminuria.

    Directory of Open Access Journals (Sweden)

    Vannina G Marrachelli

    Full Text Available To identify factors related with the risk to develop microalbuminuria using combined genomic and metabolomic values from a general population study. One thousand five hundred and two subjects, Caucasian, more than 18 years, representative of the general population, were included. Blood pressure measurement and albumin/creatinine ratio were measured in a urine sample. Using SNPlex, 1251 SNPs potentially associated to urinary albumin excretion (UAE were analyzed. Serum metabolomic profile was assessed by 1H NMR spectra using a Brucker Advance DRX 600 spectrometer. From the total population, 1217 (mean age 54 ± 19, 50.6% men, ACR>30 mg/g in 81 subjects with high genotyping call rate were analysed. A characteristic metabolomic profile, which included products from mitochondrial and extra mitochondrial metabolism as well as branched amino acids and their derivative signals, were observed in microalbuminuric as compare to normoalbuminuric subjects. The comparison of the metabolomic profile between subjects with different UAE status for each of the genotypes associated to microalbuminuria revealed two SNPs, the rs10492025_TT of RPH3A gene and the rs4359_CC of ACE gene, with minimal or no statistically significant differences. Subjects with and without microalbuminuria, who shared the same genotype and metabolomic profile, differed in age. Microalbuminurics with the CC genotype of the rs4359 polymorphism and with the TT genotype of the rs10492025 polymorphism were seven years older and seventeen years younger, respectively as compared to the whole microalbuminuric subjects. With the same metabolomic environment, characteristic of subjects with microalbuminuria, the TT genotype of the rs10492025 polymorphism seems to increase and the CC genotype of the rs4359 polymorphism seems to reduce risk to develop microalbuminuria.

  6. Distributed radiofrequency signal processing using multicore fibers

    Science.gov (United States)

    Garcia, S.; Gasulla, I.

    2016-11-01

    Next generation fiber-wireless communication paradigms will require new technologies to address the current limitations to massive capacity, connectivity and flexibility. Multicore optical fibers, which were conceived for high-capacity digital communications, can bring numerous advantages to fiber-wireless radio access architectures. Besides radio over fiber parallel distribution and multiple antenna connectivity, multicore fibers can implement, at the same time, a variety of broadband processing functionalities for microwave and millimeter-wave signals. This approach leads to the novel concept of "fiber-distributed signal processing". In particular, we capitalize on the spatial parallelism inherent to multicore fibers to implement a broadband tunable true time delay line, which is the basis of multiple processing applications such as signal filtering, arbitrary waveform generation and squint-free radio beamsteering. We present the design of trench-assisted heterogeneous multicore fibers composed of cores featuring individual spectral group delays and chromatic dispersion profiles. Besides fulfilling the requirements for true time delay line operation, the MCFs are optimized in terms of higher-order dispersion, crosstalk and bend sensitivity. Microwave photonics signal processing will benefit from the performance stability, 2D operation versatility and compactness brought by the reported fiberintegrated solution.

  7. Spiking the expectancy profiles

    DEFF Research Database (Denmark)

    Hansen, Niels Chr.; Loui, Psyche; Vuust, Peter

    Melodic expectations are generated with different degrees of certainty. Given distributions of expectedness ratings for multiple continuations of each context, as obtained with the probe-tone paradigm, this certainty can be quantified in terms of Shannon entropy. Because expectations arise from...... Kullback-Leibler or Jensen-Shannon Divergence) between listeners’ prior expectancy profiles and probability distributions of a musical style or of stimuli used in short-term experiments. Five previous probe-tone experiments with musicians and non-musicians were revisited. In Experiments 1-2 participants...... and relevance of musical training and within-participant decreases after short-term exposure to novel music. Thus, whereas inexperienced listeners make high-entropy predictions, statistical learning over varying timescales enables listeners to generate melodic expectations with reduced entropy...

  8. Profiling Expelled Students

    Directory of Open Access Journals (Sweden)

    Warnie Richardson

    2010-05-01

    Full Text Available The purpose of this study was to determine what, if any, demographic trends exist respecting students expelled for violent behavior. The data collected from 104 confidential student files were used to profile each of the following: A. The violent student, B. The nature of school violence, and C. How schools are dealing with violent students. The student expelled for violent behavior is typically male, between the ages of 15 and 18, has a history of previous suspension and has average to below-average academic skills. The incidents of violence occur in common areas of the school, are rarely directed toward staff and teachers, involve a weapon, and are classified as aggravated assaults. Schools are directly involving the police, expelling students for extended periods of time

  9. Losartan: Comprehensive Profile.

    Science.gov (United States)

    Al-Majed, Abdul-Rahman A; Assiri, Ebrahim; Khalil, Nasr Y; Abdel-Aziz, Hatem A

    2015-01-01

    Losartan (Cozaar™) is an angiotensin II receptor antagonist with antihypertensive activity. It is used in the management of hypertension and heart failure. Nomenclature, formulae, elemental analysis, and appearance of the drug are included in this review. The uses, applications, and the variety of synthetic pathways of this drug are outlined. Physical characteristics including: ionization constant, solubility, X-ray powder diffraction pattern, thermal methods of analysis, UV spectrum, IR spectrum, mass spectrum with fragmentation patterns, and NMR (1H and 13C) spectra of losartan together with the corresponding figures and/or tables are all produced. This profile also includes the monograph of British Pharmacopoeia, together with several reported analytical methods including: spectrophotometric, electrochemical, chromatographic, and capillary electrophoretic methods. The stability, the pharmacokinetic behavior and the pharmacology of the drug are also provided.

  10. Purinergic signalling and diabetes

    DEFF Research Database (Denmark)

    Burnstock, Geoffrey; Novak, Ivana

    2013-01-01

    The pancreas is an organ with a central role in nutrient breakdown, nutrient sensing and release of hormones regulating whole body nutrient homeostasis. In diabetes mellitus, the balance is broken-cells can be starving in the midst of plenty. There are indications that the incidence of diabetes...... type 1 and 2, and possibly pancreatogenic diabetes, is rising globally. Events leading to insulin secretion and action are complex, but there is emerging evidence that intracellular nucleotides and nucleotides are not only important as intracellular energy molecules but also as extracellular signalling...... molecules in purinergic signalling cascades. This signalling takes place at the level of the pancreas, where the close apposition of various cells-endocrine, exocrine, stromal and immune cells-contributes to the integrated function. Following an introduction to diabetes, the pancreas and purinergic...

  11. Positional information generated by spatially distributed signaling cascades.

    Directory of Open Access Journals (Sweden)

    Javier Muñoz-García

    2009-03-01

    Full Text Available The temporal and stationary behavior of protein modification cascades has been extensively studied, yet little is known about the spatial aspects of signal propagation. We have previously shown that the spatial separation of opposing enzymes, such as a kinase and a phosphatase, creates signaling activity gradients. Here we show under what conditions signals stall in the space or robustly propagate through spatially distributed signaling cascades. Robust signal propagation results in activity gradients with long plateaus, which abruptly decay at successive spatial locations. We derive an approximate analytical solution that relates the maximal amplitude and propagation length of each activation profile with the cascade level, protein diffusivity, and the ratio of the opposing enzyme activities. The control of the spatial signal propagation appears to be very different from the control of transient temporal responses for spatially homogenous cascades. For spatially distributed cascades where activating and deactivating enzymes operate far from saturation, the ratio of the opposing enzyme activities is shown to be a key parameter controlling signal propagation. The signaling gradients characteristic for robust signal propagation exemplify a pattern formation mechanism that generates precise spatial guidance for multiple cellular processes and conveys information about the cell size to the nucleus.

  12. Electronic signal conditioning

    CERN Document Server

    NEWBY, BRUCE

    1994-01-01

    At technician level, brief references to signal conditioning crop up in a fragmented way in various textbooks, but there has been no single textbook, until now!More advanced texts do exist but they are more mathematical and presuppose a higher level of understanding of electronics and statistics. Electronic Signal Conditioning is designed for HNC/D students and City & Guilds Electronics Servicing 2240 Parts 2 & 3. It will also be useful for BTEC National, Advanced GNVQ, A-level electronics and introductory courses at degree level.

  13. TOR signalling in plants.

    Science.gov (United States)

    Rexin, Daniel; Meyer, Christian; Robaglia, Christophe; Veit, Bruce

    2015-08-15

    Although the eukaryotic TOR (target of rapamycin) kinase signalling pathway has emerged as a key player for integrating nutrient-, energy- and stress-related cues with growth and metabolic outputs, relatively little is known of how this ancient regulatory mechanism has been adapted in higher plants. Drawing comparisons with the substantial knowledge base around TOR kinase signalling in fungal and animal systems, functional aspects of this pathway in plants are reviewed. Both conserved and divergent elements are discussed in relation to unique aspects associated with an autotrophic mode of nutrition and adaptive strategies for multicellular development exhibited by plants.

  14. Genomic signal processing

    CERN Document Server

    Shmulevich, Ilya

    2007-01-01

    Genomic signal processing (GSP) can be defined as the analysis, processing, and use of genomic signals to gain biological knowledge, and the translation of that knowledge into systems-based applications that can be used to diagnose and treat genetic diseases. Situated at the crossroads of engineering, biology, mathematics, statistics, and computer science, GSP requires the development of both nonlinear dynamical models that adequately represent genomic regulation, and diagnostic and therapeutic tools based on these models. This book facilitates these developments by providing rigorous mathema

  15. Understanding signal integrity

    CERN Document Server

    Thierauf, Stephen C

    2010-01-01

    This unique book provides you with practical guidance on understanding and interpreting signal integrity (SI) performance to help you with your challenging circuit board design projects. You find high-level discussions of important SI concepts presented in a clear and easily accessible format, including question and answer sections and bulleted lists.This valuable resource features rules of thumb and simple equations to help you make estimates of critical signal integrity parameters without using circuit simulators of CAD (computer-aided design). The book is supported with over 120 illustratio

  16. Using scale heights derived from bottomside ionograms for modelling the IRI topside profile

    Directory of Open Access Journals (Sweden)

    B. W. Reinisch

    2004-01-01

    Full Text Available Groundbased ionograms measure the Chapman scale height HT at the F2-layer peak that is used to construct the topside profile. After a brief review of the topside model extrapolation technique, comparisons are presented between the modeled profiles with incoherent scatter radar and satellite measurements for the mid latitude and equatorial ionosphere. The total electron content TEC, derived from measurements on satellite beacon signals, is compared with the height-integrated profiles ITEC from the ionograms. Good agreement is found with the ISR profiles and with results using the low altitude TOPEX satellite. The TEC values derived from GPS signal analysis are systematically larger than ITEC. It is suggested to use HT , routinely measured by a large number of Digisondes around the globe, for the construction of the IRI topside electron density profile.

  17. UGV Interoperability Profile (IOP) - Overarching Profile JAUS Profiling Rules, Version 0

    Science.gov (United States)

    2011-12-21

    this is defined as any non-variable lights associated with the vehicle forward drive camera. - Left Turn Signal – the left turn signal will be used...to turn on and off a vehicle’s left turn signal . The left turn signal is defined as a visual signal presented to an observer behind the vehicle...that indicates the vehicle is about to or is turning left. In the case of a Unclassified 40 vehicle that has no left turn signal , this field

  18. Physician Cost Profiling — Reliability and Risk of Misclassification

    Science.gov (United States)

    Adams, John L.; Mehrotra, Ateev; Thomas, J. William; McGlynn, Elizabeth A.

    2010-01-01

    BACKGROUND Insurance products with incentives for patients to choose physicians classified as offering lower-cost care on the basis of cost-profiling tools are increasingly common. However, no rigorous evaluation has been undertaken to determine whether these tools can accurately distinguish higher-cost physicians from lower-cost physicians. METHODS We aggregated claims data for the years 2004 and 2005 from four health plans in Massachusetts. We used commercial software to construct clinically homogeneous episodes of care (e.g., treatment of diabetes, heart attack, or urinary tract infection), assigned each episode to a physician, and created a summary profile of resource use (i.e., cost) for each physician on the basis of all assigned episodes. We estimated the reliability (signal-to-noise ratio) of each physician’s cost-profile score on a scale of 0 to 1, with 0 indicating that all differences in physicians’ cost profiles are due to a lack of precision in the measure (noise) and 1 indicating that all differences are due to real variation in costs of services (signal). We used the reliability results to estimate the proportion of physicians in each specialty whose cost performance would be classified inaccurately in a two-tiered insurance product in which the physicians with cost profiles in the lowest quartile were labeled as “lower cost.” RESULTS Median reliabilities ranged from 0.05 for vascular surgery to 0.79 for gastroenterology and otolaryngology. Overall, 59% of physicians had cost-profile scores with reliabilities of less than 0.70, a commonly used marker of suboptimal reliability. Using our reliability results, we estimated that 22% of physicians would be misclassified in a two-tiered system. CONCLUSIONS Current methods for profiling physicians with respect to costs of services may produce misleading results. PMID:20237347

  19. [Legal implication of DNA profiling].

    Science.gov (United States)

    Doutremepuich, Christian

    2012-06-01

    In recent years, DNA profiling has been used regularly by the justice system, and has seen a number of improvements, with the need for fewer cells, more efficient DNA extraction and purification, and more rapid genotyping. These methods can now identify an individual more rapidly, from a corpse, blood stain, sperm or epithelial cells, by comparison with familial profiles. In France, DNA profiling can only be ordered by a judge.

  20. Profiling the atmosphere with the airborne radio occultation technique

    Science.gov (United States)

    Muradyan, Paytsar

    The GNSS Instrument System for Multistatic and Occultation Sensing (GISMOS) was designed for dense sampling of meteorological targets using the airborne radio occultation (RO) technique. Airborne RO refers to an atmospheric limb sounding technique in which Global Positioning System (GPS) signals are recorded at a receiver onboard an aircraft as the satellites descend beyond the limb of the Earth. The GPS signals, that are unaffected by clouds and precipitation, experience refractive bending as well as a delay in the travel time through the atmosphere. Bending can be used to retrieve information about atmospheric refractivity, which depends on atmospheric moisture and temperature. The new system has the potential for improving numerical weather prediction (NWP) forecasts through assimilation of many high-resolution atmospheric profiles in an area of interest, compared to spaceborne RO, which samples sparsely around the globe. In February 2008, GISMOS was deployed on the National Science Foundation Gulfstream-V aircraft to make atmospheric observations in the Gulf of Mexico coastal region with an objective to test the performance of the profiling system. Recordings from this flight campaign made with the conventional phase lock loop GPS receivers descend from flight level to 5 km altitude. However, below that level strong refractivity gradients, especially those associated with the boundary layer, cause rapid phase accelerations resulting in loss of lock in the receiver. To extend the RO profiles deeper in the atmosphere, the GISMOS system was also equipped with a GPS Recording System (GRS) that records the raw RF signals. Post-processing this dataset in open-loop (OL) tracking mode enables reliable atmospheric profiling at lower altitudes. We present a comprehensive analysis of the performance of the airborne system OL tracking algorithm during a 5 hour flight on 15 February 2008. Excess phase and amplitude profiles for 5 setting and 5 rising occultations were