Maistro Edson Luis
Full Text Available Cytogenetic and morphological studies were carried out on nine local populations of Astyanax scabripinnis paranae. All populations exhibited 2n = 50 chromosomes as well as conspicuous differences involving karyotype morphology, number and position of nucleolar organizer regions (NORs and amount and/or locations of constitutive heterochromatin blocks. A quantitative study of the cytogenetic data showed that eight populations possessed different karyotypes. Morphological analyses based on nine measurements and two meristic parameters were effective in establishing clear identification of five populations. Comparative analysis of cytogenetic and morphological traits suggests that chromosomal changes have occurred at a more rapid rate than morphological differentiation. Despite the close morphological similarity found among some populations, chromosomal differentiation was identified in all of them, even in those presenting only small morphological differences.
Barbosa, Patrícia; de Oliveira, Luiz Antonio; Pucci, Marcela Baer; Santos, Mateus Henrique; Moreira-Filho, Orlando; Vicari, Marcelo Ricardo; Nogaroto, Viviane; de Almeida, Mara Cristina; Artoni, Roberto Ferreira
Most part of the eukaryotic genome is composed of repeated sequences or multiple copies of DNA, which were considered as "junk DNA", and may be associated to the heterochromatin. In this study, three populations of Astyanax aff. scabripinnis from Brazilian rivers of Guaratinguetá and Pindamonhangaba (São Paulo) and a population from Maringá (Paraná) were analyzed concerning the localization of the nucleolar organizer regions (Ag-NORs), the As51 satellite DNA, the 18S ribosomal DNA (rDNA), and the 5S rDNA. Repeated sequences were also isolated and identified by the Cot - 1 method, which indicated similarity (90%) with the LINE UnaL2 retrotransposon. The fluorescence in situ hybridization (FISH) showed the retrotransposon dispersed and more concentrated markers in centromeric and telomeric chromosomal regions. These sequences were co-localized and interspaced with 18S and 5S rDNA and As51, confirmed by fiber-FISH essay. The B chromosome found in these populations pointed to a conspicuous hybridization with LINE probe, which is also co-located in As51 sequences. The NORs were active at unique sites of a homologous pair in the three populations. There were no evidences that transposable elements and repetitive DNA had influence in the transcriptional regulation of ribosomal genes in our analyses.
Full Text Available Foram avaliadas a composição, a dieta, as táticas alimentares e ocupação espacial da ictiofauna em um pequeno riacho dentro de um remanescente de Floresta com Araucária. Seis espécies de peixes foram coletadas, pertencentes a três ordens e quatro famílias, totalizando 110 exemplares. No ambiente de corredeira foram coletados e observados apenas Trichomycterus castroi Pinna, 1992 and Trichomycterus davisi Haseman, 1911, enquanto que no ambiente de remanso foram observados e capturados exemplares de Astyanax aff. scabripinnis (sensu Eigenmann, 1921, Astyanax totae Haluch & Abilhoa, 2005, Trichomycterus castroi Pinna, 1992, T. davisi, Phalloceros caudimaculatus (Hensel, 1868 e Jenynsia eigenmanni Haseman, 1911. Dezoito itens alimentares de origem autóctone, seis de alóctone e três de origem incerta foram identificados e tiveram sua freqüência de ocorrência calculada. Uma grande quantidade de itens exclusivos foi registrada, todavia os insetos constituíram a base alimentar mais importante, principalmente na forma de fragmentos e estágios imaturos (larvas e pupas, o que permitiu inferências a respeito de sobreposição alimentar. As espécies registradas no riacho foram classificadas em quatro guildas tróficas e quatro grupos funcionais. As espécies estudadas são capazes de utilizar diversos recursos alimentares e microambientes, o que é favorecido provavelmente pelo seu pequeno porte. O remanescente florestal estudado exerce grande importância no fornecimento de alimento, hábitats e refúgios, fatores importantes para manutenção da biota aquática.The species composition, diet, feeding tactics, and spatial occupation of fishes in a headwater stream which flows within a remaining fragment of Araucaria Forest were studied. One hundred and ten fishes of six species were collected, members of three orders and four families. In riffle areas, only Trichomycterus castroi Pinna, 1992 and Trichomycterus davisi Haseman, 1911 were
Nishiyama, P B; Vieira, M M R; Porto, F E; Borin, L A; Portela-Castro, A L B; Santos, I C M
The group Incertae sedis within the Characidae family currently includes 88 genera, previously included in the subfamily Tetragonopterinae. Among them is the genus Astyanax comprising a group of species with similar morphology and widely distributed in the Neotropics. Thus, the present study aimed to analyze the karyotype diversity in Astyanax species from different watersheds by conventional Giemsa staining, C-banding and fluorescence in situ hybridization (FISH rDNA 18S) probe.specimens of Astyanax aff. paranae belonging to the "scabripinnis complex", Astyanax asunsionensis and Astyanax aff. bimaculatus were analyzed". Two sympatric karyomorphs were observed in Astyanax.aff paranae, one of them having2n=48andthe other one with 2n=50 chromosomes. Other population of this same species also presented 2n=50 chromosomes, but differing in the karyotype formula and with macro supernumerary chromosome found in 100% of the cells in about 80%of females analyzed. Two population of A. asuncionensis and one population of Astyanax. aff. bimaculatus, also showed a diploid number of 50 chromosomes, but also differing in their karyotype formulas. Therefore, A. asuncionensis was also characterized by intraspecific chromosome diversity. The C-banding analysis was able to demonstrate a distinctable to demonstrate a distinct pattern of heterochromatin differing A. asuncionensis from Astyanax aff. paranae and Astyanax aff. bimaculatus. The supernumerary chromosome of Astyanax aff. paranae proved completely heterochromatic. Only Astyanax.aff. bimaculatus multiple showed multiple sites of nucleolar organizing regions. The other species were characterized by having a simple system of NOR. These data contributes to the know ledge of the existing biodiversity in our fish fauna, here highlighted by the inter- and intraspecific chromosomal diversity in the genus Astyanax. PMID:26934153