WorldWideScience

Sample records for astatine 205

  1. Radiochemistry of astatine

    Energy Technology Data Exchange (ETDEWEB)

    Ruth, T J; Dombsky, M; D' Auria, J M; Ward, T E

    1988-01-01

    This monograph is a review of the literature through 1987 and covers the methods of producing the radioisotopes of astatine and the inorganic, nuclear, and organic chemistry of astatine. The discussion is limited to chemical and physical chemical properties of astatine. The monograph, after the introduction, is divided into chapters titled: production methods, nuclear spectroscopy, chemistry of astatine, separation and isolation (dry and wet), and selected procedures. 209 refs., 15 figs., 7 tabs. (DLC)

  2. Spectroscopy of low-lying states in neutron-deficient astatine and francium nuclei

    Energy Technology Data Exchange (ETDEWEB)

    Jakobsson, U., E-mail: ulrjak@kth.se; Cederwall, B. [KTH, The Division of Nuclear Physics, AlbaNova University Center, SE-10691 Stockholm (Sweden); Uusitalo, J.; Auranen, K.; Badran, H.; Cox, D. M.; Grahn, T.; Greenlees, P. T.; Julin, R.; Juutinen, S.; Herzáň, A.; Konki, J.; Leino, M.; Mallaburn, M.; Pakarinen, J.; Papadakis, P.; Partanen, J.; Rahkila, P.; Sandzelius, M.; Sarén, J. [University of Jyvaskyla, Department of Physics, P.O. Box 35, FI-40014 University of Jyvaskyla (Finland); and others

    2015-10-15

    Low-lying states in neutron-deficient astatine and francium nuclei have been studied by means of in-beam and delayed spectroscopy. The 13/2{sup +} state has been observed in francium nuclei with a similar down-sloping trend as in neighbouring astatine and bismuth isotopes, as a function of decreasing neutron number. A systematic trend can also now be seen for the 1/2{sup +} state both in astatine and francium nuclei, where the level energy decreases steeply as a function of neutron number when moving further away from the neutron shell closure. This trend is very similar between astatine nuclei and their francium isotones. Moreover, shape coexistence has been observed between the 13/2{sup +} state and the spherical 9/2{sup −} ground state in {sup 203}Fr and {sup 205}Fr.

  3. Spectroscopy of low-lying states in neutron-deficient astatine and francium nuclei

    Science.gov (United States)

    Jakobsson, U.; Uusitalo, J.; Auranen, K.; Badran, H.; Cederwall, B.; Cox, D. M.; Grahn, T.; Greenlees, P. T.; Julin, R.; Juutinen, S.; HerzáÅ, A.; Konki, J.; Leino, M.; Mallaburn, M.; Pakarinen, J.; Papadakis, P.; Partanen, J.; Rahkila, P.; Sandzelius, M.; Sarén, J.; Scholey, C.; Sorri, J.; Stolze, S.

    2015-10-01

    Low-lying states in neutron-deficient astatine and francium nuclei have been studied by means of in-beam and delayed spectroscopy. The 13/2+ state has been observed in francium nuclei with a similar down-sloping trend as in neighbouring astatine and bismuth isotopes, as a function of decreasing neutron number. A systematic trend can also now be seen for the 1/2+ state both in astatine and francium nuclei, where the level energy decreases steeply as a function of neutron number when moving further away from the neutron shell closure. This trend is very similar between astatine nuclei and their francium isotones. Moreover, shape coexistence has been observed between the 13/2+ state and the spherical 9/2- ground state in 203Fr and 205Fr.

  4. Discovery of the astatine, radon, francium, and radium isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Fry, C.; Thoennessen, M., E-mail: thoennessen@nscl.msu.edu

    2013-09-15

    Thirty-nine astatine, thirty-nine radon, thirty-five francium, and thirty-four radium isotopes have so far been observed; the discovery of these isotopes is described. For each isotope a brief summary of the first refereed publication, including the production and identification method, is presented.

  5. Discovery of the astatine, radon, francium, and radium isotopes

    CERN Document Server

    Fry, C

    2012-01-01

    Currently, thirty-nine astatine, thirty-nine radon, thirty-five francium, and thirty-four radium isotopes have so far been observed; the discovery of these isotopes is discussed. For each isotope a brief summary of the first refereed publication, including the production and identification method, is presented.

  6. Discovery of the astatine, radon, francium, and radium isotopes

    Science.gov (United States)

    Fry, C.; Thoennessen, M.

    2013-09-01

    Thirty-nine astatine, thirty-nine radon, thirty-five francium, and thirty-four radium isotopes have so far been observed; the discovery of these isotopes is described. For each isotope a brief summary of the first refereed publication, including the production and identification method, is presented.

  7. Delayed and In-beam Spectroscopy on Francium and Astatine Nuclei at the Proton Drip Line

    Energy Technology Data Exchange (ETDEWEB)

    Uusitalo, J.; Jakobsson, U. [Department of Physics, University of Jyvaeskylae (Finland); Collaboration: RITU-Gamma Gollaboration

    2011-11-30

    Delayed and in-beam spectroscopy on francium and astatine nuclei at and beyond the proton drip line has been performed. In neutron deficient astatine nuclei a shift to deformed shapes as a function of decreasing neutron has been obtained. In neutron deficient francium isotope the same shift is evident.

  8. Delayed and In-beam Spectroscopy on Francium and Astatine Nuclei at the Proton Drip Line

    Science.gov (United States)

    Uusitalo, J.; Jakobsson, U.

    2011-11-01

    Delayed and in-beam spectroscopy on francium and astatine nuclei at and beyond the proton drip line has been performed. In neutron deficient astatine nuclei a shift to deformed shapes as a function of decreasing neutron has been obtained. In neutron deficient francium isotope the same shift is evident.

  9. Measurement of the first ionization potential of astatine by laser ionization spectroscopy

    CERN Document Server

    Rothe, S; Antalic, S; Borschevsky, A; Capponi, L; Cocolios, T E; De Witte, H; Eliav, E; Fedorov, D V; Fedosseev, V N; Fink, D A; Fritzsche, S; Ghys, L; Huyse, M; Imai, N; Kaldor, U; Kudryavtsev, Yu; Köster, U; Lane, J; Lassen, J; Liberati, V; Lynch, K M; Marsh, B A; Nishio, K; Pauwels, D; Pershina, V; Popescu, L; Procter, T J; Radulov, D; Raeder, S; Rajabali, M M; Rapisarda, E; Rossel, R E; Sandhu, K; Seliverstov, M D; Sjödin, A M; Van den Bergh, P; Van Duppen, P; Venhart, M; Wakabayashi, Y; Wendt K D A

    2013-01-01

    The radioactive element astatine exists only in trace amounts in nature. Its properties can therefore only be explored by study of smallest quantities of artificially produced isotopes or by performing theoretical calculations. One of the most important properties influencing the chemical behaviour is the energy required to remove one electron from the valence shell, referred to as the ionization potential. Here we use laser spectroscopy to probe the optical spectrum of astatine near the ionization threshold. The observed series of Rydberg states enabled the first determination of the ionization potential of the astatine atom, 9.317510(8) eV. New ab initio calculations were performed to support the experimental result. The measured value serves as a benchmark for quantum chemistry calculations of the properties of astatine as well as for the theoretical prediction of the ionization potential of super-heavy element 117, the heaviest homologue of astatine.

  10. Measurement of the first ionization potential of astatine by laser ionization spectroscopy.

    Science.gov (United States)

    Rothe, S; Andreyev, A N; Antalic, S; Borschevsky, A; Capponi, L; Cocolios, T E; De Witte, H; Eliav, E; Fedorov, D V; Fedosseev, V N; Fink, D A; Fritzsche, S; Ghys, L; Huyse, M; Imai, N; Kaldor, U; Kudryavtsev, Yuri; Köster, U; Lane, J F W; Lassen, J; Liberati, V; Lynch, K M; Marsh, B A; Nishio, K; Pauwels, D; Pershina, V; Popescu, L; Procter, T J; Radulov, D; Raeder, S; Rajabali, M M; Rapisarda, E; Rossel, R E; Sandhu, K; Seliverstov, M D; Sjödin, A M; Van den Bergh, P; Van Duppen, P; Venhart, M; Wakabayashi, Y; Wendt, K D A

    2013-01-01

    The radioactive element astatine exists only in trace amounts in nature. Its properties can therefore only be explored by study of the minute quantities of artificially produced isotopes or by performing theoretical calculations. One of the most important properties influencing the chemical behaviour is the energy required to remove one electron from the valence shell, referred to as the ionization potential. Here we use laser spectroscopy to probe the optical spectrum of astatine near the ionization threshold. The observed series of Rydberg states enabled the first determination of the ionization potential of the astatine atom, 9.31751(8) eV. New ab initio calculations are performed to support the experimental result. The measured value serves as a benchmark for quantum chemistry calculations of the properties of astatine as well as for the theoretical prediction of the ionization potential of superheavy element 117, the heaviest homologue of astatine.

  11. Automated astatination of biomolecules - a stepping stone towards multicenter clinical trials

    DEFF Research Database (Denmark)

    Aneheim, Emma; Albertsson, Per; Bäck, Tom

    2015-01-01

    To facilitate multicentre clinical studies on targeted alpha therapy, it is necessary to develop an automated, on-site procedure for conjugating rare, short-lived, alpha-emitting radionuclides to biomolecules. Astatine-211 is one of the few alpha-emitting nuclides with appropriate chemical...

  12. Measurement of the first ionization potential of astatine by laser ionization spectroscopy

    NARCIS (Netherlands)

    Rothe, S.; Andreyev, A. N.; Antalic, S.; Borschevsky, A.; Capponi, L.; Cocolios, T. E.; De Witte, H.; Eliav, E.; Fedorov, D. V.; Fedosseev, V. N.; Fink, D. A.; Fritzsche, S.; Ghys, L.; Huyse, M.; Imai, N.; Kaldor, U.; Kudryavtsev, Yuri; Koester, U.; Lane, J. F. W.; Lassen, J.; Liberati, V.; Lynch, K. M.; Marsh, B. A.; Nishio, K.; Pauwels, D.; Pershina, V.; Popescu, L.; Procter, T. J.; Radulov, D.; Raeder, S.; Rajabali, M. M.; Rapisarda, E.; Rossel, R. E.; Sandhu, K.; Seliverstov, M. D.; Sjoedin, A. M.; Van den Bergh, P.; Van Duppen, P.; Venhart, M.; Wakabayashi, Y.; Wendt, K. D. A.

    The radioactive element astatine exists only in trace amounts in nature. Its properties can therefore only be explored by study of the minute quantities of artificially produced isotopes or by performing theoretical calculations. One of the most important properties influencing the chemical

  13. An attempt to explore the production routes of Astatine radionuclides: Theoretical approach

    OpenAIRE

    Maiti, Moumita; Lahiri, Susanta

    2008-01-01

    In order to fulfil the recent thrust of Astatine radionuclides in the field of nuclear medicine various production routes have been explored in the present work. The possible production routes of $^{209-211}$At comprise both light and heavy ion induced reactions at the bombarding energy range starting from threshold to maximum 100 MeV energy. For this purpose, we have used the nuclear reaction model codes TALYS, ALICE91 and PACE-II. Excitation functions of those radionuclides, produced throug...

  14. Synthesis and Evaluation of Astatinated N-[2-(Maleimido)ethyl]-3-(trimethylstannyl)benzamide Immunoconjugates

    DEFF Research Database (Denmark)

    Aneheim, Emma; Gustafsson, Anna; Albertsson, Per

    2016-01-01

    Effective treatment of metastasis is a great challenge in the treatment of different types of cancers. Targeted alpha therapy utilizes the short tissue range (50-100 μm) of α particles, making the method suitable for treatment of disseminated occult cancers in the form of microtumors or even sing...... of the in vivo distribution of the new immunoconjugate with other tin-based immunoconjugates in tumor-bearing mice, the MSB conjugation method was found to be a viable option for successful astatine labeling of different monoclonal antibodies....

  15. Laser photodetachment of radioactive ions: towards the determination of the electronegativity of astatine

    CERN Multimedia

    Rothe, Sebastian; Welander, Jakob Emanuel; Chrysalidis, Katerina; Day Goodacre, Thomas; Fedosseev, Valentine; Fiotakis, Spyridon; Forstner, Oliver; Heinke, Reinhard Matthias; Johnston, Karl; Kron, Tobias; Koester, Ulli; Liu, Yuan; Marsh, Bruce; Ringvall Moberg, Annie; Rossel, Ralf Erik; Seiffert, Christoph; Studer, Dominik; Wendt, Klaus; Hanstorp, Dag

    2017-01-01

    Negatively charged ions are mainly stabilized through the electron correlation effect. A measure of the stability of a negative ion is the electron affinity, which the energy gain by attaching an electron to a neutral atom. This fundamental quantity is, due to the almost general lack of bound excited states, the only atomic property that can be determined with high accuracy for negative ions. We will present the results of the first laser photodetachment studies of radioactive negative ions at CERN-ISOLDE. The photodetachment threshold for the radiogenic iodine isotope 128I was measured successfully, demonstrating the performance of the upgraded GANDALPH experimental beam line. The first detection of photo-detached astatine atoms marks a milestone towards the determination of the EA of this radioactive element.

  16. Complexation study on no-carrier-added astatine with insulin: A candidate radiopharmaceutical

    Energy Technology Data Exchange (ETDEWEB)

    Lahiri, Susanta [Chemical Sciences Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700 064 (India)], E-mail: susanta.lahiri@saha.ac.in; Roy, Kamalika [Chemical Sciences Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700 064 (India); Sen, Souvik [Berhampur Sadar Hospital, Berhampur, Murshidabad 742 101 (India)

    2008-12-15

    No-carrier-added astatine radionuclides produced in the {sup 7}Li-irradiated lead matrix were separated from bulk lead nitrate target by complexing At with insulin, followed by dialysis. The method offers simultaneous separation of At from lead as well as its complexation with insulin. The At-insulin complex might be a potential radiopharmaceutical in the treatment of hepatocellular carcinoma. The stability of At-insulin complex was checked by dialysis against deionized water and Ringer lactate (RL) solution. It has been found that the half-life of At-insulin complex is about {approx}12 h, when dialyzed against deionized water and is only 6 h, when dialyzed against RL solution having the same composition as blood serum. The 6 h half-life of this Insulin-At complex is perfect for killing cancer cells from external cell surfaces as the half-life of internalization of insulin molecule inside the cell is 7-12 h.

  17. Determination of the electron affinity of astatine and polonium by laser photodetachment

    CERN Multimedia

    We propose to conduct the first electron affinity (EA) measurements of the two elements astatine (At) and polonium (Po). Collinear photo-detachment spectroscopy will allow us to measure these quantities with an uncertainty limited only by the spectral line width of the laser. We plan to use negative ion beams of the two radioactive elements At and Po, which are only accessible on-line and at ISOLDE. The feasibility of our proposed method and the functionality of the experimental setup have been demonstrated at ISOLDE in off-line tests by the clear observation of the photo-detachment threshold for stable iodine. This proposal is based on our Letter of Intent I-148.

  18. Adsorption of the astatine species on a gold surface: A relativistic density functional theory study

    Science.gov (United States)

    Demidov, Yuriy; Zaitsevskii, Andréi

    2018-01-01

    We report first-principle based studies of the adsorption interaction of astatine species on a gold surface. These studies are aimed primarily at the support and interpretation of gas chromatographic experiments with superheavy elements, tennessine (Ts, Z = 117), a heavier homologue of At, and possibly its pseudo-homologue nihonium (Nh, Z = 113). We use gold clusters with up to 69 atoms to simulate the adsorption sites and estimate the desorption energies of At & AtOH from a stable gold (1 1 1) surface. To describe the electronic structure of At -Aun and AtOH -Aun complexes, we combine accurate shape-consistent relativistic pseudopotentials and non-collinear two-component relativistic density functional theory. The predicted desorption energies of At and AtOH on gold are 130 ± 10 kJ/mol and 90 ± 10 kJ/mol, respectively. These results confirm the validity of the estimates derived from chromatographic data (147 ± 15 kJ/mol for At, and 100-10+20 kJ/mol for AtOH).

  19. ASTATINE-211 RADIOCHEMISTRY: THE DEVELOPMENT OF METHODOLOGIES FOR HIGH ACTIVITY LEVEL RADIOSYNTHESIS

    Energy Technology Data Exchange (ETDEWEB)

    MICHAEL R. ZALUTSKY

    2012-08-08

    Targeted radionuclide therapy is emerging as a viable approach for cancer treatment because of its potential for delivering curative doses of radiation to malignant cell populations while sparing normal tissues. Alpha particles such as those emitted by 211At are particularly attractive for this purpose because of their short path length in tissue and high energy, making them highly effective in killing cancer cells. The current impact of targeted radiotherapy in the clinical domain remains limited despite the fact that in many cases, potentially useful molecular targets and labeled compounds have already been identified. Unfortunately, putting these concepts into practice has been impeded by limitations in radiochemistry methodologies. A critical problem is that the synthesis of therapeutic radiopharmaceuticals provides additional challenges in comparison to diagnostic reagents because of the need to perform radio-synthesis at high levels of radioactivity. This is particularly important for {alpha}-particle emitters such as 211At because they deposit large amounts of energy in a highly focal manner. The overall objective of this project is to develop convenient and reproducible radiochemical methodologies for the radiohalogenation of molecules with the {alpha}-particle emitter 211At at the radioactivity levels needed for clinical studies. Our goal is to address two problems in astatine radiochemistry: First, a well known characteristic of 211At chemistry is that yields for electrophilic astatination reactions decline as the time interval after radionuclide isolation from the cyclotron target increases. This is a critical problem that must be addressed if cyclotrons are to be able to efficiently supply 211At to remote users. And second, when the preparation of high levels of 211At-labeled compounds is attempted, the radiochemical yields can be considerably lower than those encountered at tracer dose. For these reasons, clinical evaluation of promising 211At

  20. An all-solid state laser system for the laser ion sources RILIS and in-source laser spectroscopy of astatine at ISOLDE/CERN

    Energy Technology Data Exchange (ETDEWEB)

    Rothe, Sebastian

    2012-09-24

    This doctoral thesis describes the extension of the resonance ionization laser ion source RILIS at CERN/ISOLDE by the addition of an all-solid state tunable titanium:sapphire (Ti:Sa) laser system to complement the well-established system of dye lasers. Synchronous operation of the so called Dual RILIS system of Ti:Sa and dye lasers was investigated and the potential for increased ion beam intensity, reliability, and reduced setup time has been demonstrated. In-source resonance ionization spectroscopy was performed at ISOLDE/CERN and at ISAC/TRIUMF radioactive ion beam facilities to develop an efficient and selective three-colour ionization scheme for the purely radioactive element astatine. A LabVIEW based monitoring, control and measurement system was conceived which enabled, in conjunction with Dual RILIS operation, the spectroscopy of high lying Rydberg states, from which the ionization potential of the astatine atom was determined for the first time experimentally.

  1. An all-solid state laser system for the laser ion source RILIS and in-source laser spectroscopy of astatine at ISOLDE, CERN

    CERN Document Server

    Rothe, Sebastian; Nörtershäuser, W

    This doctoral thesis describes the extension of the resonance ionization laser ion source RILIS at ISOLDE, CERN, by the addition of an all-solid state tuneable titanium: sapphire (Ti:Sa) laser system to complement the well-established system of dye lasers. Synchronous operation of the so called Dual RILIS system of Ti:Sa and dye lasers was investigated and the potential for increased ion beam intensity, reliability, and reduced setup time has been demonstrated. In-source resonance ionization spectroscopy was performed at ISOLDE, CERN, and at ISAC, TRIUMF, radioactive ion beam facilities to develop an efficient and selective three-colour ionization scheme for the purely radioactive element astatine. A LabVIEW based monitoring, control and measurement system was conceived which enabled, in conjunction with Dual RILIS operation, the spectroscopy of high lying Rydberg states, from which the ionization potential of the astatine atom was determined for the first time experimentally.

  2. 7 CFR 205.691-205.699 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.691-205.699 Section 205.691-205.699 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  3. 7 CFR 205.240-205.269 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.240-205.269 Section 205.240-205.269 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  4. 7 CFR 205.673-205.679 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.673-205.679 Section 205.673-205.679 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  5. 7 CFR 205.291-205.299 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.291-205.299 Section 205.291-205.299 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  6. 7 CFR 205.682-205.689 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.682-205.689 Section 205.682-205.689 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  7. 7 CFR 205.407-205.499 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.407-205.499 Section 205.407-205.499 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  8. 7 CFR 205.208-205.235 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.208-205.235 Section 205.208-205.235 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  9. 7 CFR 205.643-205.649 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.643-205.649 Section 205.643-205.649 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  10. 7 CFR 205.666-205.667 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.666-205.667 Section 205.666-205.667 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  11. 7 CFR 205.623-205.639 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.623-205.639 Section 205.623-205.639 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  12. 7 CFR 205.608-205.619 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.608-205.619 Section 205.608-205.619 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  13. 7 CFR 205.106-205.199 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.106-205.199 Section 205.106-205.199 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  14. 7 CFR 205.511-205.599 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.511-205.599 Section 205.511-205.599 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  15. 7 CFR 205.273-205.289 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.273-205.289 Section 205.273-205.289 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  16. 7 CFR 205.205 - Crop rotation practice standard.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Crop rotation practice standard. 205.205 Section 205.205 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC...

  17. 7 CFR 205.312-205.399 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.312-205.399 Section 205.312-205.399 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  18. Study of Neutron-Deficient $^{202-205}$Fr Isotopes with Collinear Resonance Ionization Spectroscopy

    CERN Document Server

    De Schepper, Stijn; Cocolios, Thomas; Budincevic, Ivan

    The scope of this master’s thesis is the study of neutron-deficient $^{202−205}$Fr isotopes. These isotopes are inside the neutron-deficient lead region, a region that has shown evidence of shape coexistence. For this thesis, this discussion is limited to the phenomenon where a low lying excited state has a different shape than the ground state. Shape coexistence is caused by intruder states. These are single-particle Shell Model states that are perturbed in energy due to the interaction with a deformed core. In the neutron-deficient lead region the main proton intruder orbit is the 3s$_{1/2}$orbit. When going towards more neutron-deficient isotopes, deformation increases. The $\\pi3s_{1/2}$orbit will rise in energy and will eventually become the ground state in odd- A bismuth (Z=83) isotopes. It is also observed in odd-A astatine (Z=85) isotopes, already in less neutron-deficient nuclei. The same phenomenon is expected to be present francium (Z=87) isotopes already at $^{199}$Fr. Although it is currently ...

  19. Final Report for research grant "Development of Methods for High Specific Activity Labeling of Biomolecules Using Astatine-211 in Different Oxidation States"

    Energy Technology Data Exchange (ETDEWEB)

    Wilbur, D. Scott [Univ. of Washington, Seattle, WA (United States)

    2011-12-14

    The overall objective of this research effort was to develop methods for labeling biomolecules with higher oxidation state species of At-211. This was to be done in an effort to develop reagents that had higher in vivo stability than the present carbon-bonded At-211-labeled compounds. We were unsuccessful in that effort, as none of the approaches studied provided reagents that were stable to in vivo deastatination. However, we gained a lot of information about At-211 in higher oxidation states. The studies proved to be very difficult as small changes in pH and other conditions appeared to change the nature of the species that obtained (by HPLC retention time analyses), with many of the species being unidentifiable. The fact that there are no stable isotopes of astatine, and the chemistry of the nearest halogen iodine is quite different, made it very difficult to interpret results of some experiments. With that said, we believe that a lot of valuable information was obtained from the studies. The research effort evaluated: (1) methods for chemical oxidation of At-211, (2) approaches to chelation of oxidized At-211, and (3) approaches to oxidation of astatophenyl compounds. A major hurdle that had to be surmounted to conduct the research was the development of HPLC conditions to separate and identify the various oxidized species formed. Attempts to develop conditions for separation of iodine and astatine species by normal and reversed-phase TLC and ITLC were not successful. However, we were successful in developing conditions (from a large number of attempts) to separate oxidized forms of iodine ([I-125]iodide, [I-125]iodate and [I-125]periodate) and astatine ([At-211]astatide, [At-211]astatate, [At-211]perastatate, and several unidentified At-211 species). Information on the basic oxidation and characterization of At-211 species is provided under Objective 1. Conditions were developed to obtain new At-211 labeling method where At-211 is chelated with the DOTA and

  20. 32 CFR 205.5 - Responsibilities.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Responsibilities. 205.5 Section 205.5 National... USE CERTIFICATES (EUCs) § 205.5 Responsibilities. (a) The Under Secretary of Defense (Acquisition... in § 205.6. This responsibility may not be delegated by the Directors of Defense Agencies; it may be...

  1. An automated flow system incorporating in-line acid dissolution of bismuth metal from a cyclotron irradiated target assembly for use in the isolation of astatine-211

    Energy Technology Data Exchange (ETDEWEB)

    O’Hara, Matthew J.; Krzysko, Anthony J.; Niver, Cynthia M.; Morrison, Samuel S.; Owsley, Stanley L.; Hamlin, Donald K.; Dorman, Eric F.; Scott Wilbur, D.

    2017-04-01

    Astatine-211 (211At) is a promising cyclotron-produced radionuclide being investigated for use in targeted alpha therapy of blood borne and metastatic cancers, as well as treatment of tumor remnants after surgical resections. The isolation of trace quantities of 211At, produced within several grams of a Bi metal cyclotron target, involves a complex, multi-step procedure: (1) Bi metal dissolution in strong HNO3, (2) distillation of the HNO3 to yield Bi salts containing 211At, (3) dissolution of the salts in strong HCl, (4) solvent extraction of 211At from bismuth salts with diisopropyl ether (DIPE), and (5) back-extraction of 211At from DIPE into NaOH, leading to a purified 211At product. Step (1) has been addressed first to begin the process of automating the onerous 211At isolation process. A computer-controlled Bi target dissolution system has been designed. The system performs in-line dissolution of Bi metal from the target assembly using an enclosed target dissolution block, routing the resulting solubilized 211At/Bi mixture to the subsequent process step. The primary parameters involved in Bi metal solubilization (HNO3 concentration and influent flow rate) were optimized prior to evaluation of the system performance on replicate cyclotron irradiated targets. The results indicate that the system performs reproducibly, having nearly quantitative release of 211At from irradiated targets, with cumulative 211At recoveries that follow a sigmoidal function. The predictable nature of the 211At release profile allows the user to tune the system to meet target processing requirements.

  2. Reagents for astatination of biomolecules. 2. Conjugation of anionic boron cage pendant groups to a protein provides a method for direct labeling that is stable to in vivo deastatination.

    Science.gov (United States)

    Wilbur, D Scott; Chyan, Ming-Kuan; Hamlin, Donald K; Vessella, Robert L; Wedge, Timothy J; Hawthorne, M Frederick

    2007-01-01

    Cancer-targeting biomolecules labeled with 211At must be stable to in vivo deastatination, as control of the 211At distribution is critical due to the highly toxic nature of alpha-particle emission. Unfortunately, no astatinated aryl conjugates have shown in vivo stability toward deastatination when (relatively) rapidly metabolized proteins, such as monoclonal antibody Fab' fragments, are labeled. As a means of increasing the in vivo stability of 211At-labeled proteins, we have been investigating antibody conjugates of boron cage moieties. In this investigation, protein-reactive derivatives containing a nido-carborane (2), a bis-nido-carborane derivative (Venus Flytrap Complex, 3), and four 2-nonahydro-closo-decaborate(2-) derivatives (4-7) were prepared and conjugated with an antibody Fab' fragment such that subsequent astatination and in vivo tissue distributions could be obtained. To aid in determination of stability toward in vivo deastatination, the Fab'-borane conjugates were also labeled with 125I, and that material was coinjected with the 211At-labeled Fab'. For comparison, direct labeling of the Fab' with 125I and 211At was conducted. Direct labeling with Na[125I]I and Chloramine-T gave an 89% radiochemical yield. However, direct labeling of the Fab' with Na[211At]At and Chloramine-T resulted in a yield of Studies to optimize the closo-decaborate(2-) conjugates for protein labeling are underway.

  3. Dicty_cDB: VHB205 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available library) VHB205 (Link to dictyBase) - - - Contig-U16486-1 VHB205P (Link to Original site) VHB205F 655 VHB205Z...VHB205Z 703 VHB205P 1338 - - Show VHB205 Library VH (Link to library) Clone ID VHB205 (Link to dictyBase)...biol.tsukuba.ac.jp/CSM/VH/VHB2-A/VHB205Q.Seq.d/ Representative seq. ID VHB205P (Link to Original site)...site) Representative DNA sequence >VHB205 (VHB205Q) /CSM/VH/VHB2-A/VHB205Q.Seq.d/ ATTTAGAATATTTTAGATTAAA...significant alignments: (bits) Value VHB205 (VHB205Q) /CSM/VH/VHB2-A/VHB205Q.Seq.d/ 2613 0.0 VSD867 (VSD867Q)

  4. 7 CFR 205.301 - Product composition.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Product composition. 205.301 Section 205.301 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... PROVISIONS NATIONAL ORGANIC PROGRAM Labels, Labeling, and Market Information § 205.301 Product composition...

  5. 50 CFR 223.205 - Sea turtles.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Sea turtles. 223.205 Section 223.205... Threatened Marine and Anadromous Species § 223.205 Sea turtles. (a) The prohibitions of section 9 of the Act (16 U.S.C. 1538) relating to endangered species apply to threatened species of sea turtle, except as...

  6. 12 CFR 347.205 - Record maintenance.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Record maintenance. 347.205 Section 347.205 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION REGULATIONS AND STATEMENTS OF GENERAL POLICY INTERNATIONAL BANKING Foreign Banks § 347.205 Record maintenance. The records of each insured branch shall be...

  7. 5 CFR 530.205 - Records.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Records. 530.205 Section 530.205 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS PAY RATES AND SYSTEMS (GENERAL) Aggregate Limitation on Pay § 530.205 Records. An agency must maintain appropriate records to administer...

  8. 15 CFR 280.205 - Representation.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Representation. 280.205 Section 280.205 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade NATIONAL INSTITUTE... QUALITY Enforcement § 280.205 Representation. A respondent individual may appear and participate in person...

  9. 40 CFR 90.205 - Banking.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Banking. 90.205 Section 90.205... EMISSIONS FROM NONROAD SPARK-IGNITION ENGINES AT OR BELOW 19 KILOWATTS Certification Averaging, Banking, and Trading Provisions § 90.205 Banking. (a)(1) Beginning August 1, 2007, a manufacturer of a Class I engine...

  10. 40 CFR 89.205 - Banking.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Banking. 89.205 Section 89.205... EMISSIONS FROM NEW AND IN-USE NONROAD COMPRESSION-IGNITION ENGINES Averaging, Banking, and Trading Provisions § 89.205 Banking. (a) Requirements for Tier 1 engines rated at or above 37 kW. (1) A manufacturer...

  11. 40 CFR 91.205 - Banking.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Banking. 91.205 Section 91.205... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Averaging, Banking, and Trading Provisions § 91.205 Banking. (a... banking. (i) For outboard engines in model year (MY) 1997, a manufacturer may bank positive emission...

  12. 32 CFR 205.3 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Definitions. 205.3 Section 205.3 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS END USE CERTIFICATES (EUCs) § 205.3 Definitions. (a) End Use Certificate (EUC). For the purposes of this...

  13. 10 CFR § 205.83 - Contents.

    Science.gov (United States)

    2016-01-01

    ... 10 Energy 3 2016-01-01 2016-01-01 false Contents. § 205.83 Section § 205.83 Energy DEPARTMENT OF ENERGY OIL ADMINISTRATIVE PROCEDURES AND SANCTIONS Interpretation § 205.83 Contents. (a) The request shall contain a full and complete statement of all relevant facts pertaining to the circumstances, act...

  14. 10 CFR 205.7 - Service.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 3 2010-01-01 2010-01-01 false Service. 205.7 Section 205.7 Energy DEPARTMENT OF ENERGY OIL ADMINISTRATIVE PROCEDURES AND SANCTIONS General Provisions § 205.7 Service. (a) All orders... personally or by registered or certified mail or by regular United States mail (only when service is effected...

  15. 27 CFR 41.205 - [Reserved

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false 41.205 Section 41.205 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE... TOBACCO Tobacco Products Importers Required Records and Reports § 41.205 Filing and Retention of Records...

  16. 40 CFR 205.58-1 - Warranty.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Warranty. 205.58-1 Section 205.58-1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.58-1 Warranty. (a) The vehicle manufacturer...

  17. 40 CFR 205.58-2 - Tampering.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Tampering. 205.58-2 Section 205.58-2 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.58-2 Tampering. (a) For each configuration...

  18. 40 CFR 205.55 - Requirements.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Requirements. 205.55 Section 205.55 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.55 Requirements. ...

  19. 40 CFR 205.50 - Applicability.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Applicability. 205.50 Section 205.50 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.50 Applicability. (a) Except as otherwise...

  20. 40 CFR 205.51 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Definitions. 205.51 Section 205.51 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.51 Definitions. (a) As used in this subpart...

  1. 40 CFR 205.54 - Test procedures.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Test procedures. 205.54 Section 205.54 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.54 Test procedures. The procedures described...

  2. 40 CFR 240.205 - Air quality.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Air quality. 240.205 Section 240.205 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES GUIDELINES FOR THE THERMAL PROCESSING OF SOLID WASTES Requirements and Recommended Procedures § 240.205 Air quality. ...

  3. 20 CFR 655.205 - Recruitment period.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Recruitment period. 655.205 Section 655.205... Agricultural Employment § 655.205 Recruitment period. (a) If the OFLC Administrator determines that the... carry out the assurances contained in § 655.203 with respect to the recruitment of U.S. workers. The...

  4. 40 CFR 205.168 - Requirements.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Requirements. 205.168 Section 205.168 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Motorcycle Exhaust Systems § 205.168 Requirements. ...

  5. 40 CFR 205.157 - Requirements.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Requirements. 205.157 Section 205.157 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Motorcycles § 205.157 Requirements. ...

  6. 42 CFR 66.205 - Requirements.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Requirements. 66.205 Section 66.205 Public Health... NATIONAL RESEARCH SERVICE AWARDS Institutional Grants § 66.205 Requirements. (a) No Award shall be made to... the form and manner the Secretary may prescribe, that he or she will satisfy the requirements of § 66...

  7. 48 CFR 1443.205 - Contract clauses.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contract clauses. 1443.205 Section 1443.205 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR CONTRACT MANAGEMENT CONTRACT MODIFICATIONS Change Orders 1443.205 Contract clauses. BPCs may establish procedures, when...

  8. 47 CFR 20.5 - Citizenship.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false Citizenship. 20.5 Section 20.5... SERVICES § 20.5 Citizenship. (a) This rule implements section 310 of the Communications Act, 47 U.S.C. 310, regarding the citizenship of licensees in the commercial mobile radio services. Commercial mobile radio...

  9. Dicty_cDB: SLF205 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available SL (Link to library) SLF205 (Link to dictyBase) - - - Contig-U16054-1 SLF205Z (Link... to Original site) - - SLF205Z 590 - - - - Show SLF205 Library SL (Link to library) Clone ID SLF205 (Link to...ycdb.biol.tsukuba.ac.jp/CSM/SL/SLF2-A/SLF205Q.Seq.d/ Representative seq. ID SLF20...5Z (Link to Original site) Representative DNA sequence >SLF205 (SLF205Q) /CSM/SL/SLF2-A/SLF205Q.Seq.d/ XXXXX...AATGATGATT sequence update 1999. 7.15 Translated Amino Acid sequence ---TLDEGTYIVIPSTYDHGIEGAFHLTLFTDDKNATFQ

  10. 45 CFR 205.150 - Cost allocation.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Cost allocation. 205.150 Section 205.150 Public... ASSISTANCE PROGRAMS § 205.150 Cost allocation. A State plan under title I, IV-A, X, XIV, or XVI (AABD) of the Social Security Act must provide that the State agency will have an approved cost allocation plan on file...

  11. 24 CFR 206.205 - Property charges.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Property charges. 206.205 Section... CONVERSION MORTGAGE INSURANCE Servicing Responsibilities § 206.205 Property charges. (a) General. The mortgagor shall pay all property charges consisting of taxes, ground rents, flood and hazard insurance...

  12. 48 CFR 1631.205-41 - Taxes.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Taxes. 1631.205-41 Section 1631.205-41 Federal Acquisition Regulations System OFFICE OF PERSONNEL MANAGEMENT FEDERAL EMPLOYEES... imposition of taxes, fees, or other monetary payment, directly or indirectly, on FEHB premiums by any State...

  13. 48 CFR 2131.205-41 - Taxes.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Taxes. 2131.205-41 Section 2131.205-41 Federal Acquisition Regulations System OFFICE OF PERSONNEL MANAGEMENT, FEDERAL EMPLOYEES....S.C. 8714(c) or other Federal law prohibits the imposition of taxes, fees, or other monetary...

  14. 7 CFR 205.290 - Temporary variances.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Temporary variances. 205.290 Section 205.290 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Organic...

  15. 7 CFR 205.503 - Applicant information.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Applicant information. 205.503 Section 205.503 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  16. 7 CFR 205.200 - General.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false General. 205.200 Section 205.200 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Organic Production...

  17. 7 CFR 205.664 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.664 Section 205.664 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Administrative...

  18. 7 CFR 205.506 - Granting accreditation.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Granting accreditation. 205.506 Section 205.506 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  19. 7 CFR 205.663 - Mediation.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Mediation. 205.663 Section 205.663 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Administrative...

  20. 7 CFR 205.311 - USDA Seal.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false USDA Seal. 205.311 Section 205.311 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Labels, Labeling,...

  1. 7 CFR 205.669 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.669 Section 205.669 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Administrative...

  2. 7 CFR 205.237 - Livestock feed.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Livestock feed. 205.237 Section 205.237 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Organic...

  3. 7 CFR 205.104 - [Reserved

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false [Reserved] 205.104 Section 205.104 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Applicability § 20...

  4. 7 CFR 205.508 - Site evaluations.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Site evaluations. 205.508 Section 205.508 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  5. 7 CFR 205.202 - Land requirements.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Land requirements. 205.202 Section 205.202 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Organic...

  6. 24 CFR 1006.205 - Development.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Development. 1006.205 Section 1006.205 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR PUBLIC AND INDIAN HOUSING, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  7. 31 CFR 800.205 - Conversion.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Conversion. 800.205 Section 800.205 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF INVESTMENT SECURITY, DEPARTMENT OF THE TREASURY REGULATIONS PERTAINING TO MERGERS, ACQUISITIONS, AND TAKEOVERS BY...

  8. 49 CFR 1560.205 - Redress process.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false Redress process. 1560.205 Section 1560.205... Redress process. (a) If an individual believes he or she has been improperly or unfairly delayed or... individual may seek assistance through the redress process established under this section. (b) An individual...

  9. 40 CFR 205.153 - Engine displacement.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Engine displacement. 205.153 Section... TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Motorcycles § 205.153 Engine displacement. (a) Engine displacement must be calculated using nominal engine values and rounded to the nearest whole cubic centimeter...

  10. 41 CFR 101-39.205 - [Reserved

    Science.gov (United States)

    2010-07-01

    ... PROPERTY MANAGEMENT REGULATIONS AVIATION, TRANSPORTATION, AND MOTOR VEHICLES 39-INTERAGENCY FLEET MANAGEMENT SYSTEMS 39.2-GSA Interagency Fleet Management System Services § 101-39.205 ... 41 Public Contracts and Property Management 2 2010-07-01 2010-07-01 true 101-39.205 Section 101-39...

  11. 48 CFR 1851.205 - Contract clause.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Contract clause. 1851.205 Section 1851.205 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION CONTRACT MANAGEMENT USE OF GOVERNMENT SOURCES BY CONTRACTORS Contractor Use of Interagency Fleet Management...

  12. 48 CFR 31.205-11 - Depreciation.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Depreciation. 31.205-11....205-11 Depreciation. (a) Depreciation on a contractor's plant, equipment, and other capital facilities... method of depreciation or the class life asset depreciation range system is used, the residual value need...

  13. 38 CFR 3.205 - Marriage.

    Science.gov (United States)

    2010-07-01

    ... specifically reciting the effects of the decree. (c) Marriages deemed valid. Where a surviving spouse has... 38 Pensions, Bonuses, and Veterans' Relief 1 2010-07-01 2010-07-01 false Marriage. 3.205 Section 3..., Compensation, and Dependency and Indemnity Compensation Evidence Requirements § 3.205 Marriage. (a) Proof of...

  14. 24 CFR 103.205 - Systemic processing.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Systemic processing. 103.205... HOUSING FAIR HOUSING-COMPLAINT PROCESSING Investigation Procedures § 103.205 Systemic processing. Where... are pervasive or institutional in nature, or that the processing of the complaint will involve complex...

  15. 40 CFR 6.205 - Environmental assessments.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Environmental assessments. 6.205... ACTIONS EPA's NEPA Environmental Review Procedures § 6.205 Environmental assessments. (a) The Responsible Official must prepare an environmental assessment (EA) (see 40 CFR 1508.9) for a proposed action that is...

  16. 9 CFR 205.103 - EFS-minimum information.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false EFS-minimum information. 205.103 Section 205.103 Animals and Animal Products GRAIN INSPECTION, PACKERS AND STOCKYARDS ADMINISTRATION... EFS, is to be subject to the particular security interest; (2) Farm product name (see §§ 205.106, 205...

  17. 24 CFR 585.205 - Eligible activities.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 3 2010-04-01 2010-04-01 false Eligible activities. 585.205... activities. Planning grant activities to develop a Youthbuild program may include: (a) The undertaking of... development training, counseling, and other supportive services and activities for the Youthbuild program...

  18. 27 CFR 25.205 - Production.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Production. 25.205 Section... Production. (a) Any adult may produce beer, without payment of tax, for personal or family use and not for... that age before commencing the production of beer. This exemption does not authorize the production of...

  19. 40 CFR 205.151 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... motorcycle that: (A) Has an engine displacement less than 50 cubic centimeters; (B) Produces no more than two... maintained, must continue in compliance with the Federal standard. (8) Advertised Engine Displacement means... subpart. (14) Engine displacement means volumetric engine capacity as defined in § 205.153. (15) Exhaust...

  20. 7 CFR 205.404 - Granting certification.

    Science.gov (United States)

    2010-01-01

    ... must issue a certificate of organic operation which specifies the: (1) Name and address of the..., Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Certification § 205.404 Granting certification. (a) Within a reasonable...

  1. 48 CFR 251.205 - Contract clause.

    Science.gov (United States)

    2010-10-01

    ... Fleet Management System (IFMS) Vehicles 251.205 Contract clause. Use the clause at 252.251-7001, Use of Interagency Fleet Management System (IFMS)Vehicles and Related Services, in solicitations and contracts which include the clause at FAR 52.251-2, Interagency Fleet Management System (IFMS) Vehicles and Related...

  2. 24 CFR 904.205 - Training methodology.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Training methodology. 904.205... Training methodology. Equal in importance to the content of the pre- and post-occupancy training is the training methodology. Because groups vary, there should be adaptability in the communication and learning...

  3. 27 CFR 9.205 - Chehalem Mountains.

    Science.gov (United States)

    2010-04-01

    ... located in Clackamas, Yamhill, and Washington Counties, Oregon. The boundary of the Chehalem Mountains... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Chehalem Mountains. 9.205... Chehalem Mountains. (a) Name. The name of the viticultural area described in this section is “Chehalem...

  4. 9 CFR 113.205 - Newcastle Disease Vaccine, Killed Virus.

    Science.gov (United States)

    2010-01-01

    ... Virus. 113.205 Section 113.205 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS STANDARD REQUIREMENTS Killed Virus Vaccines § 113.205 Newcastle Disease Vaccine, Killed Virus. Newcastle Disease Vaccine...

  5. 46 CFR 502.205 - Interrogatories to parties.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 9 2010-10-01 2010-10-01 false Interrogatories to parties. 502.205 Section 502.205... Depositions, Written Interrogatories, and Discovery § 502.205 Interrogatories to parties. (a) Service; answers. (1) Any party may serve, pursuant to subpart H of this part, upon any other party written...

  6. 49 CFR 382.205 - On-duty use.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false On-duty use. 382.205 Section 382.205 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY... ALCOHOL USE AND TESTING Prohibitions § 382.205 On-duty use. No driver shall use alcohol while performing...

  7. 48 CFR 231.205-22 - Legislative lobbying costs.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Legislative lobbying costs. 231.205-22 Section 231.205-22 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS... Contracts With Commercial Organizations 231.205-22 Legislative lobbying costs. (a) Costs associated with...

  8. 7 CFR 58.205 - Meaning of words.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Meaning of words. 58.205 Section 58.205 Agriculture... Whole Milk, and Dry Buttermilk § 58.205 Meaning of words. For the purpose of the regulations in this subpart, words in the singular form shall be deemed to impart the plural and vice versa, as the case may...

  9. 7 CFR 205.1 - Meaning of words.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Meaning of words. 205.1 Section 205.1 Agriculture... PROVISIONS NATIONAL ORGANIC PROGRAM Definitions § 205.1 Meaning of words. For the purpose of the regulations in this subpart, words in the singular form shall be deemed to impart the plural and vice versa, as...

  10. 46 CFR 16.205 - Implementation of chemical testing programs.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Implementation of chemical testing programs. 16.205 Section 16.205 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN CHEMICAL TESTING Required Chemical Testing § 16.205 Implementation of chemical testing programs. (a) When a...

  11. 46 CFR 205.5 - Contracts containing disputes article.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false Contracts containing disputes article. 205.5 Section 205... AUDIT APPEALS; POLICY AND PROCEDURE § 205.5 Contracts containing disputes article. When a contract contains a disputes article, the disputes article will govern the bases for negotiating disputes regarding...

  12. 49 CFR 375.205 - May I have agents?

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false May I have agents? 375.205 Section 375.205... General Responsibilities § 375.205 May I have agents? (a) You may have agents provided you comply with... performs such services only on an emergency or temporary basis. (b) If you have agents, you must have...

  13. 48 CFR 1631.205-10 - Cost of money.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Cost of money. 1631.205-10... AND PROCEDURES Contracts With Commercial Organizations 1631.205-10 Cost of money. For the purposes of FAR 31.205-10(b)(3), the estimated facilities capital cost of money is specifically identified if it...

  14. 40 CFR 205.3 - Number and gender.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Number and gender. 205.3 Section 205.3... EQUIPMENT NOISE EMISSION CONTROLS General Provisions § 205.3 Number and gender. As used in this part, words in the -singular shall be deemed to import -the plural, and words in the masculine -gender shall be...

  15. 29 CFR 780.205 - Nursery activities generally.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Nursery activities generally. 780.205 Section 780.205 Labor... as It Relates to Specific Situations Nursery and Landscaping Operations § 780.205 Nursery activities generally. The employees of a nursery who are engaged in the following activities are employed in...

  16. 45 CFR 205.130 - State financial participation.

    Science.gov (United States)

    2010-10-01

    ... ADMINISTRATION-PUBLIC ASSISTANCE PROGRAMS § 205.130 State financial participation. State plan requirements: (a) A State plan for financial assistance under title I, IV-A, X, XIV, or XVI (AABD) of the Social Security... 45 Public Welfare 2 2010-10-01 2010-10-01 false State financial participation. 205.130 Section 205...

  17. 45 CFR 205.30 - Methods of administration.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Methods of administration. 205.30 Section 205.30 Public Welfare Regulations Relating to Public Welfare OFFICE OF FAMILY ASSISTANCE (ASSISTANCE PROGRAMS), ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION-PUBLIC ASSISTANCE PROGRAMS § 205.30...

  18. 48 CFR 31.205-51 - Costs of alcoholic beverages.

    Science.gov (United States)

    2010-10-01

    ... Organizations 31.205-51 Costs of alcoholic beverages. Costs of alcoholic beverages are unallowable. ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Costs of alcoholic beverages. 31.205-51 Section 31.205-51 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION...

  19. 40 CFR 300.205 - Planning and coordination structure.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 27 2010-07-01 2010-07-01 false Planning and coordination structure. 300.205 Section 300.205 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... POLLUTION CONTINGENCY PLAN Planning and Preparedness § 300.205 Planning and coordination structure. (a...

  20. 40 CFR 205.59 - Recall of noncomplying vehicles.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Recall of noncomplying vehicles. 205.59 Section 205.59 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.59 Recall...

  1. 40 CFR 205.57 - Selective enforcement auditing requirements.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Selective enforcement auditing requirements. 205.57 Section 205.57 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.57...

  2. 40 CFR 205.55-2 - Compliance with standards.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Compliance with standards. 205.55-2 Section 205.55-2 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.55-2 Compliance...

  3. 40 CFR 205.54-2 - Sound data acquisition system.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Sound data acquisition system. 205.54-2 Section 205.54-2 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.54-2 Sound...

  4. 40 CFR 205.57-1 - Test request.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Test request. 205.57-1 Section 205.57-1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.57-1 Test request. (a) The...

  5. 40 CFR 205.55-3 - Configuration identification.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Configuration identification. 205.55-3 Section 205.55-3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.55-3 Configuration...

  6. 40 CFR 205.55-5 - Labeling-exterior. [Reserved

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Labeling-exterior. 205.55-5 Section 205.55-5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.55-5 Labeling...

  7. 40 CFR 205.57-8 - Continued testing.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Continued testing. 205.57-8 Section 205.57-8 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.57-8 Continued...

  8. 40 CFR 205.57-4 - Testing procedures.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Testing procedures. 205.57-4 Section 205.57-4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.57-4 Testing...

  9. 40 CFR 205.52 - Vehicle noise emission standards.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Vehicle noise emission standards. 205.52 Section 205.52 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.52 Vehicle...

  10. 40 CFR 205.57-2 - Test vehicle sample selection.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Test vehicle sample selection. 205.57-2 Section 205.57-2 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.57-2 Test...

  11. 40 CFR 205.58 - In-use requirements.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false In-use requirements. 205.58 Section 205.58 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.58 In-use requirements. ...

  12. 40 CFR 205.57-3 - Test vehicle preparation.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Test vehicle preparation. 205.57-3 Section 205.57-3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.57-3 Test vehicle...

  13. 40 CFR 205.55-4 - Labeling-compliance.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Labeling-compliance. 205.55-4 Section 205.55-4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.55-4 Labeling...

  14. 40 CFR 205.56 - Testing by the Administrator.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Testing by the Administrator. 205.56 Section 205.56 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.56 Testing by the...

  15. 40 CFR 205.55-1 - General requirements.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false General requirements. 205.55-1 Section 205.55-1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.55-1 General...

  16. 42 CFR 402.205 - Length of exclusion.

    Science.gov (United States)

    2010-10-01

    ... maximum time limit for the period of exclusion. Social Security Actparagraph Code of Federal... 42 Public Health 2 2010-10-01 2010-10-01 false Length of exclusion. 402.205 Section 402.205 Public... PROVISIONS CIVIL MONEY PENALTIES, ASSESSMENTS, AND EXCLUSIONS Exclusions § 402.205 Length of exclusion. The...

  17. 49 CFR 238.205 - Anti-climbing mechanism.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Anti-climbing mechanism. 238.205 Section 238.205... Equipment § 238.205 Anti-climbing mechanism. (a) Except as provided in paragraph (b) of this section, all..., 2010, shall have at both the forward and rear ends an anti-climbing mechanism capable of resisting an...

  18. 48 CFR 731.205-46 - Travel costs.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Travel costs. 731.205-46 Section 731.205-46 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT GENERAL....205-46 Travel costs. It is USAID policy to require prior written approval of international travel by...

  19. 48 CFR 615.205-70 - Use of English language.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Use of English language. 615.205-70 Section 615.205-70 Federal Acquisition Regulations System DEPARTMENT OF STATE CONTRACTING... Information 615.205-70 Use of English language. The requirements of DOSAR 614.201-70 also apply when...

  20. Analysis list: Su(var)205 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Su(var)205 Adult,Embryo,Larvae + dm3 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3.../target/Su(var)205.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/target/Su(var)205.5.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/dm3/target/Su(var)205.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/c...olo/Su(var)205.Adult.tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/colo/Su(v...ar)205.Embryo.tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/colo/Su(var)205.Larvae.tsv http://dbarchive.bioscience

  1. 9 CFR 205.209 - Amendment or continuation of EFS.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Amendment or continuation of EFS. 205.209 Section 205.209 Animals and Animal Products GRAIN INSPECTION, PACKERS AND STOCKYARDS... the security interest in question. That will vary from case to case. The basis for this is the purpose...

  2. 40 CFR 205.171-3 - Test motorcycle sample selection.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Test motorcycle sample selection. 205... ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Motorcycle Exhaust Systems § 205.171-3 Test motorcycle sample selection. A test motorcycle to be used for selective enforcement audit testing...

  3. 7 CFR 3560.205 - Rent and utility allowance changes.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 15 2010-01-01 2010-01-01 false Rent and utility allowance changes. 3560.205 Section..., DEPARTMENT OF AGRICULTURE DIRECT MULTI-FAMILY HOUSING LOANS AND GRANTS Rents § 3560.205 Rent and utility allowance changes. (a) General. Borrowers must fully document that changes to rents and utility allowances...

  4. 7 CFR 205.500 - Areas and duration of accreditation.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Areas and duration of accreditation. 205.500 Section 205.500 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC...

  5. 7 CFR 205.103 - Recordkeeping by certified operations.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Recordkeeping by certified operations. 205.103 Section 205.103 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANI...

  6. 7 CFR 205.239 - Livestock living conditions.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Livestock living conditions. 205.239 Section 205.239 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  7. 7 CFR 205.100 - What has to be certified.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false What has to be certified. 205.100 Section 205.100 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  8. 7 CFR 205.661 - Investigation of certified operations.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Investigation of certified operations. 205.661 Section 205.661 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANI...

  9. 7 CFR 205.403 - On-site inspections.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false On-site inspections. 205.403 Section 205.403 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  10. 7 CFR 205.306 - Labeling of livestock feed.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Labeling of livestock feed. 205.306 Section 205.306 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  11. 7 CFR 205.271 - Facility pest management practice standard.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Facility pest management practice standard. 205.271 Section 205.271 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL...

  12. 7 CFR 205.502 - Applying for accreditation.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Applying for accreditation. 205.502 Section 205.502 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  13. 7 CFR 205.505 - Statement of agreement.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Statement of agreement. 205.505 Section 205.505 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  14. 7 CFR 205.662 - Noncompliance procedure for certified operations.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Noncompliance procedure for certified operations. 205.662 Section 205.662 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS...

  15. 7 CFR 205.405 - Denial of certification.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Denial of certification. 205.405 Section 205.405 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  16. 7 CFR 205.671 - Exclusion from organic sale.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Exclusion from organic sale. 205.671 Section 205.671 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  17. 7 CFR 205.507 - Denial of accreditation.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Denial of accreditation. 205.507 Section 205.507 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  18. 7 CFR 205.401 - Application for certification.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Application for certification. 205.401 Section 205.401 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRA...

  19. 7 CFR 205.270 - Organic handling requirements.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Organic handling requirements. 205.270 Section 205.270 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRA...

  20. 7 CFR 205.620 - Requirements of State organic programs.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Requirements of State organic programs. 205.620 Section 205.620 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL...

  1. 7 CFR 205.607 - Amending the National List.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Amending the National List. 205.607 Section 205.607 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  2. 7 CFR 205.400 - General requirements for certification.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false General requirements for certification. 205.400 Section 205.400 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL...

  3. 7 CFR 205.402 - Review of application.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Review of application. 205.402 Section 205.402 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  4. 7 CFR 205.504 - Evidence of expertise and ability.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Evidence of expertise and ability. 205.504 Section 205.504 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC...

  5. 7 CFR 205.101 - Exemptions and exclusions from certification.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Exemptions and exclusions from certification. 205.101 Section 205.101 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL...

  6. 7 CFR 205.672 - Emergency pest or disease treatment.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Emergency pest or disease treatment. 205.672 Section 205.672 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC...

  7. 7 CFR 205.690 - OMB control number.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false OMB control number. 205.690 Section 205.690 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM...

  8. 40 CFR 205.5-2 - National security exemptions.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false National security exemptions. 205.5-2... PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS General Provisions § 205.5-2 National security... a national security exemption is required. (c) For purposes of section 11(d) of the Act, any...

  9. 48 CFR 31.205-10 - Cost of money.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Cost of money. 31.205-10....205-10 Cost of money. (a) General. Cost of money— (1) Is an imputed cost that is not a form of...) Refers to— (i) Facilities capital cost of money (48 CFR 9904.414); and (ii) Cost of money as an element...

  10. 5 CFR 591.205 - Which areas are nonforeign areas?

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Which areas are nonforeign areas? 591.205... ALLOWANCES AND DIFFERENTIALS Cost-of-Living Allowance and Post Differential-Nonforeign Areas Cost-Of-Living Allowances and Post Differentials § 591.205 Which areas are nonforeign areas? (a) The nonforeign areas are...

  11. 48 CFR 31.205-30 - Patent costs.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Patent costs. 31.205-30....205-30 Patent costs. (a) The following patent costs are allowable to the extent that they are incurred... patent application where title or royalty-free license is to be conveyed to the Government. (b) General...

  12. 28 CFR 16.205 - Closed meetings-Informal procedures.

    Science.gov (United States)

    2010-07-01

    ....205 Section 16.205 Judicial Administration DEPARTMENT OF JUSTICE PRODUCTION OR DISCLOSURE OF MATERIAL... business meeting of the Commission (held at least quarterly) (28 CFR 2.27). The National Appeals Board... quarterly. (b) Meetings to which applicable. The following types of meetings may be closed in the event that...

  13. 5 CFR 339.205 - Medical evaluation programs.

    Science.gov (United States)

    2010-01-01

    ... program must be clearly supported by the nature of the work. The specific positions covered must be... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Medical evaluation programs. 339.205 Section 339.205 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS MEDICAL...

  14. 21 CFR 1311.205 - Pharmacy application requirements.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 9 2010-04-01 2010-04-01 false Pharmacy application requirements. 1311.205... ELECTRONIC ORDERS AND PRESCRIPTIONS (Eff. 6-1-10) Electronic Prescriptions § 1311.205 Pharmacy application requirements. (a) The pharmacy may only use a pharmacy application that meets the requirements in paragraph (b...

  15. 45 CFR 205.101 - Organization for administration.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Organization for administration. 205.101 Section 205.101 Public Welfare Regulations Relating to Public Welfare OFFICE OF FAMILY ASSISTANCE (ASSISTANCE PROGRAMS), ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION-PUBLIC ASSISTANCE PROGRAMS §...

  16. 32 CFR 205.4 - Background and policy.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Background and policy. 205.4 Section 205.4 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED... in international agreements, by allies and friends, of the need for flexibility in the authorized...

  17. 45 CFR 205.52 - Furnishing of social security numbers.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Furnishing of social security numbers. 205.52... GENERAL ADMINISTRATION-PUBLIC ASSISTANCE PROGRAMS § 205.52 Furnishing of social security numbers. The State plan under title I, IV-A, X, XIV, or CVI (AABD) of the Social Security Act must provide that: (a...

  18. 17 CFR 205.3 - Issuer as client.

    Science.gov (United States)

    2010-04-01

    ... 17 Commodity and Securities Exchanges 2 2010-04-01 2010-04-01 false Issuer as client. 205.3... ISSUER § 205.3 Issuer as client. (a) Representing an issuer. An attorney appearing and practicing before...'s clients. (b) Duty to report evidence of a material violation. (1) If an attorney, appearing and...

  19. 48 CFR 31.205-34 - Recruitment costs.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Recruitment costs. 31.205....205-34 Recruitment costs. (a) Subject to paragraph (b) of this subsection, the following costs are... positions; or (2) Includes material that is not relevant for recruitment purposes, such as extensive...

  20. 48 CFR 1631.205-75 - Selling costs.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Selling costs. 1631.205-75 Section 1631.205-75 Federal Acquisition Regulations System OFFICE OF PERSONNEL MANAGEMENT FEDERAL... advertising costs’, and The Federal Employees Health Benefits Handbook for Personnel and Payroll Offices...

  1. 14 CFR 302.205 - Economic data and other facts.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Economic data and other facts. 302.205... Certificate and Foreign Air Carrier Permit Licensing Proceedings § 302.205 Economic data and other facts. Whenever economic data and other facts are provided in any pleading, such information shall include enough...

  2. 45 CFR 1168.205 - Professional and technical services.

    Science.gov (United States)

    2010-10-01

    ... professional legal services. Similarly, communications with the intent to influence made by an engineer... 45 Public Welfare 3 2010-10-01 2010-10-01 false Professional and technical services. 1168.205... Activities by Own Employees § 1168.205 Professional and technical services. (a) The prohibition on the use of...

  3. 45 CFR 604.205 - Professional and technical services.

    Science.gov (United States)

    2010-10-01

    ... professional legal services. Similarly, communications with the intent to influence made by an engineer... 45 Public Welfare 3 2010-10-01 2010-10-01 false Professional and technical services. 604.205... FOUNDATION NEW RESTRICTIONS ON LOBBYING Activities by Own Employees § 604.205 Professional and technical...

  4. 45 CFR 1158.205 - Professional and technical services.

    Science.gov (United States)

    2010-10-01

    ... professional legal services. Similarly, communications with the intent to influence made by an engineer... 45 Public Welfare 3 2010-10-01 2010-10-01 false Professional and technical services. 1158.205... Own Employees § 1158.205 Professional and technical services. (a) The prohibition on the use of...

  5. 48 CFR 31.205-26 - Material costs.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Material costs. 31.205-26....205-26 Material costs. (a) Material costs include the costs of such items as raw materials, parts... material costs, the contractor shall consider reasonable overruns, spoilage, or defective work (unless...

  6. 14 CFR 13.205 - Administrative law judges.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Administrative law judges. 13.205 Section 13.205 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION PROCEDURAL... Administrative law judges. (a) Powers of an administrative law judge. In accordance with the rules of this...

  7. 20 CFR 725.205 - Determination of dependency; spouse.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Determination of dependency; spouse. 725.205 Section 725.205 Employees' Benefits EMPLOYMENT STANDARDS ADMINISTRATION, DEPARTMENT OF LABOR FEDERAL COAL... Determination of dependency; spouse. For the purposes of augmenting benefits, an individual who is the miner's...

  8. 5 CFR 9701.205 - Bar on collective bargaining.

    Science.gov (United States)

    2010-01-01

    ... Section 9701.205 Administrative Personnel DEPARTMENT OF HOMELAND SECURITY HUMAN RESOURCES MANAGEMENT SYSTEM (DEPARTMENT OF HOMELAND SECURITY-OFFICE OF PERSONNEL MANAGEMENT) DEPARTMENT OF HOMELAND SECURITY HUMAN RESOURCES MANAGEMENT SYSTEM Classification General § 9701.205 Bar on collective bargaining. As...

  9. 49 CFR 20.205 - Professional and technical services.

    Science.gov (United States)

    2010-10-01

    ... professional legal services. Similarly, communications with the intent to influence made by an engineer... 49 Transportation 1 2010-10-01 2010-10-01 false Professional and technical services. 20.205... Activities by Own Employees § 20.205 Professional and technical services. (a) The prohibition on the use of...

  10. Dicty_cDB: VSC205 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available VS (Link to library) VSC205 (Link to dictyBase) - - - Contig-U16468-1 VSC205E (Link to Original site)...- - - - - - VSC205E 188 Show VSC205 Library VS (Link to library) Clone ID VSC205 (Link to dictyBase)...BB170002B10H04 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170002B10H04...|BI511643.1 BB160006B10B07 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160006B10B07...|BI515784.1 BB160020B10A04 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160020B10A04

  11. 10 CFR 205.192A - Burden of proof.

    Science.gov (United States)

    2010-01-01

    ... Remedial Order proceeding the ERA has the burden of establishing a prima facie case as to the validity of... supplemental information that may be made available under § 205.193A. (b) Once a prima facie case has been...

  12. 45 CFR 205.60 - Reports and maintenance of records.

    Science.gov (United States)

    2010-10-01

    ... PROGRAMS), ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION-PUBLIC ASSISTANCE PROGRAMS § 205.60 Reports and maintenance of records. A State plan under title I... modified, and the dollar value of these denials, terminations and modifications. Under this requirement...

  13. Page 1 Studies on Niobium and Tantalum—1 205 REFERENCES ...

    Indian Academy of Sciences (India)

    Studies on Niobium and Tantalum—1 205. REFERENCES. Abegg and Bodlander ... Z. Amorg. Chem., 1899, 20, 471. Cordier and Murgier ... Compt. rend., 1941, 213, 836. : Edmister and Albritton ... J. Amer. Chem. Soc., 1932, 54, 438. Gernez ... Compt, rend., 1887, 104, 783; 105, 803; 1888, 106, 1527. Jorissen, et al.

  14. 40 CFR 205.155 - Motorcycle class and manufacturer abbreviation.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Motorcycle class and manufacturer...) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Motorcycles § 205.155 Motorcycle class and manufacturer abbreviation. (a) Motorcycles must be grouped into classes determined by...

  15. 10 CFR 590.205 - Notice of applications.

    Science.gov (United States)

    2010-01-01

    ... WITH RESPECT TO THE IMPORT AND EXPORT OF NATURAL GAS Applications for Authorization To Import or Export Natural Gas § 590.205 Notice of applications. (a) Upon receipt of an application, the FE shall publish a... comments on specific issues or matters of fact, law, or policy raised by the application. (b) The notice of...

  16. 24 CFR 572.205 - Planning grants-eligible activities.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 3 2010-04-01 2010-04-01 false Planning grants-eligible activities... Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR COMMUNITY PLANNING AND DEVELOPMENT, DEPARTMENT OF... (HOPE 3) Grants § 572.205 Planning grants—eligible activities. Any planning grants made by HUD under the...

  17. 48 CFR 31.205-12 - Economic planning costs.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Economic planning costs... Organizations 31.205-12 Economic planning costs. Economic planning costs are the costs of general long-range management planning that is concerned with the future overall development of the contractor's business and...

  18. 20 CFR 627.205 - Public service employment prohibition.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Public service employment prohibition. 627....205 Public service employment prohibition. No funds available under titles I, II-A, II-C, or III-A of the Act may be used for public service employment (sections 141(p) and 314(d)(2)). ...

  19. 14 CFR 125.205 - Equipment requirements: Airplanes under IFR.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Equipment requirements: Airplanes under IFR... CERTIFICATION AND OPERATIONS: AIRPLANES HAVING A SEATING CAPACITY OF 20 OR MORE PASSENGERS OR A MAXIMUM PAYLOAD... Equipment Requirements § 125.205 Equipment requirements: Airplanes under IFR. No person may operate an...

  20. 44 CFR 206.205 - Payment of claims.

    Science.gov (United States)

    2010-10-01

    ... provisions of the FEMA-State Agreement, and that payments for that project have been made in accordance with... HOMELAND SECURITY DISASTER ASSISTANCE FEDERAL DISASTER ASSISTANCE Public Assistance Project Administration § 206.205 Payment of claims. (a) Small Projects. Final payment of the Federal share of these projects...

  1. 7 CFR 3550.205 - Delinquency workout agreements.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 15 2010-01-01 2010-01-01 false Delinquency workout agreements. 3550.205 Section 3550... Delinquency workout agreements. Borrowers with past due accounts may be offered the opportunity to avoid liquidation by entering into a delinquency workout agreement that specifies a plan for bringing the account...

  2. 47 CFR 90.205 - Power and antenna height limits.

    Science.gov (United States)

    2010-10-01

    ... 90.205 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO... MHz. The maximum effective radiated power (ERP) for stations operating on fixed frequencies is 300.... (d) 150-174 MHz. (1) The maximum allowable station ERP is dependent upon the station's antenna HAAT...

  3. 5 CFR 880.205 - Determinations of death.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 2 2010-01-01 2010-01-01 false Determinations of death. 880.205 Section... Determinations of death. OPM does not make findings of presumed death. A claimant for CSRS, FERS, or FEGLI death... § 880.207 must submit a death certificate or other legal certification of death issued by an authorized...

  4. 48 CFR 31.205-35 - Relocation costs.

    Science.gov (United States)

    2010-10-01

    ... immediate family (see 31.205-46) and transportation of the household and personal effects to the new... fees, points, and finance charges), except that these costs, when added to the costs described in... incident to relocation, such as disconnecting and connecting household appliances; automobile registration...

  5. 29 CFR 1603.205 - Separation of functions.

    Science.gov (United States)

    2010-07-01

    ... EXEMPT STATE AND LOCAL GOVERNMENT EMPLOYEE COMPLAINTS OF EMPLOYMENT DISCRIMINATION UNDER SECTION 304 OF THE GOVERNMENT EMPLOYEE RIGHTS ACT OF 1991 Hearings § 1603.205 Separation of functions. (a) The... employee engaged in investigating complaints under this part. (b) No Commission employee engaged in...

  6. 50 CFR 222.205 - Import and export requirements.

    Science.gov (United States)

    2010-10-01

    ... neither endangered nor threatened species and that are imported for purposes of human or animal... ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS GENERAL ENDANGERED AND THREATENED MARINE SPECIES Certificates of Exemption for Pre-Act Endangered Species Parts § 222.205 Import and export requirements. (a...

  7. 45 CFR 1230.205 - Professional and technical services.

    Science.gov (United States)

    2010-10-01

    ... contract is allowable. However, communications with the intent to influence made by a professional (such as... 45 Public Welfare 4 2010-10-01 2010-10-01 false Professional and technical services. 1230.205... Professional and technical services. (a) The prohibition on the use of appropriated funds, in § 1230.100 (a...

  8. 43 CFR 18.205 - Professional and technical services.

    Science.gov (United States)

    2010-10-01

    ... professional legal services. Similarly, communications with the intent to influence made by an engineer... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Professional and technical services. 18... LOBBYING Activities by Own Employees § 18.205 Professional and technical services. (a) The prohibition on...

  9. 45 CFR 205.36 - State plan requirements.

    Science.gov (United States)

    2010-10-01

    ... account for— (1) All the factors in the total eligibility determination process under the plan for aid, including but not limited to: (i) Identifiable correlation factors (such as social security numbers, names... ASSISTANCE PROGRAMS § 205.36 State plan requirements. A State plan under title IV-A of the Social Security...

  10. 31 CFR 594.205 - Evasions; attempts; conspiracies.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Evasions; attempts; conspiracies. 594... Prohibitions § 594.205 Evasions; attempts; conspiracies. (a) Except as otherwise authorized, and... avoids, has the purpose of evading or avoiding, or attempts to violate any of the prohibitions set forth...

  11. 31 CFR 543.205 - Evasions; attempts; conspiracies.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Evasions; attempts; conspiracies. 543... Prohibitions § 543.205 Evasions; attempts; conspiracies. (a) Except as otherwise authorized, and..., has the purpose of evading or avoiding, or attempts to violate any of the prohibitions set forth in...

  12. 31 CFR 546.205 - Evasions; attempts; conspiracies.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Evasions; attempts; conspiracies. 546... § 546.205 Evasions; attempts; conspiracies. (a) Except as otherwise authorized, and notwithstanding any... purpose of evading or avoiding, or attempts to violate any of the prohibitions set forth in this part is...

  13. 31 CFR 544.205 - Evasions; attempts; conspiracies.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Evasions; attempts; conspiracies. 544... SANCTIONS REGULATIONS Prohibitions § 544.205 Evasions; attempts; conspiracies. (a) Except as otherwise... that evades or avoids, has the purpose of evading or avoiding, or attempts to violate any of the...

  14. 31 CFR 586.205 - Evasions; attempts; conspiracies.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Evasions; attempts; conspiracies. 586... & MONTENEGRO) KOSOVO SANCTIONS REGULATIONS Prohibitions § 586.205 Evasions; attempts; conspiracies. Any... of evading or avoiding, or attempts to violate, any of the prohibitions set forth in this part is...

  15. 31 CFR 548.205 - Evasions; attempts; conspiracies.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Evasions; attempts; conspiracies. 548... § 548.205 Evasions; attempts; conspiracies. (a) Except as otherwise authorized, and notwithstanding any... purpose of evading or avoiding, or attempts to violate any of the prohibitions set forth in this part is...

  16. 31 CFR 542.205 - Evasions; attempts; conspiracies.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Evasions; attempts; conspiracies. 542... § 542.205 Evasions; attempts; conspiracies. (a) Except as otherwise authorized, and notwithstanding any... the purpose of evading or avoiding, or attempts to violate any of the prohibitions set forth in this...

  17. 31 CFR 595.205 - Evasions; attempts; conspiracies.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Evasions; attempts; conspiracies. 595... § 595.205 Evasions; attempts; conspiracies. Any transaction for the purpose of, or which has the effect... set forth in this part, is hereby prohibited. Any attempt to violate the prohibitions set forth in...

  18. 31 CFR 547.205 - Evasions; attempts; conspiracies.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Evasions; attempts; conspiracies. 547... REGULATIONS Prohibitions § 547.205 Evasions; attempts; conspiracies. (a) Except as otherwise authorized, and..., has the purpose of evading or avoiding, or attempts to violate any of the prohibitions set forth in...

  19. 14 CFR 67.205 - Ear, nose, throat, and equilibrium.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Ear, nose, throat, and equilibrium. 67.205..., nose, throat, and equilibrium. Ear, nose, throat, and equilibrium standards for a second-class airman..., vertigo or a disturbance of equilibrium. ...

  20. 48 CFR 53.205-1 - Paid advertisements.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Paid advertisements. 53... (CONTINUED) CLAUSES AND FORMS FORMS Prescription of Forms 53.205-1 Paid advertisements. SF 1449, prescribed in 53.212, shall be used to place orders for paid advertisements as specified in 5.503. ...

  1. 40 CFR 205.157-3 - Configuration identification.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Configuration identification. 205.157... identification. (a) A separate vehicle configuration shall be determined by each combination of the following parameters: (1) Exhaust system (engine): (i) Mufflers; (ii) expansion chambers; (iii) spark arrestors; and...

  2. 42 CFR 418.205 - Special requirements for hospice pre-election evaluation and counseling services.

    Science.gov (United States)

    2010-10-01

    ... evaluation and counseling services. 418.205 Section 418.205 Public Health CENTERS FOR MEDICARE & MEDICAID... Services § 418.205 Special requirements for hospice pre-election evaluation and counseling services. (a... evaluation and counseling services as specified in § 418.304(d) may be made to a hospice on behalf of a...

  3. 9 CFR 205.105 - Master list and portion thereof distributed to registrants-format.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Master list and portion thereof distributed to registrants-format. 205.105 Section 205.105 Animals and Animal Products GRAIN INSPECTION... product to a security interest (see § 205.102), in numerical order by social security number or approved...

  4. 40 CFR 205.57-6 - Acceptance and rejection of batches.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Acceptance and rejection of batches. 205.57-6 Section 205.57-6 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.57-6...

  5. 40 CFR 205.58-3 - Instructions for maintenance, use and repair.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Instructions for maintenance, use and repair. 205.58-3 Section 205.58-3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205...

  6. 40 CFR 205.57-5 - Reporting of the test results.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Reporting of the test results. 205.57-5 Section 205.57-5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.57-5...

  7. 40 CFR 205.57-9 - Prohibition on distribution in commerce; manufacturer's remedy.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Prohibition on distribution in commerce; manufacturer's remedy. 205.57-9 Section 205.57-9 Protection of Environment ENVIRONMENTAL... Medium and Heavy Trucks § 205.57-9 Prohibition on distribution in commerce; manufacturer's remedy. (a...

  8. 40 CFR 205.57-7 - Acceptance and rejection of batch sequence.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Acceptance and rejection of batch sequence. 205.57-7 Section 205.57-7 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205...

  9. 40 CFR 205.163 - Recall of noncomplying motorcycles; relabeling of mislabeled motorcycles.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Recall of noncomplying motorcycles; relabeling of mislabeled motorcycles. 205.163 Section 205.163 Protection of Environment ENVIRONMENTAL... Motorcycles § 205.163 Recall of noncomplying motorcycles; relabeling of mislabeled motorcycles. (a) Pursuant...

  10. 31 CFR 205.11 - What requirements apply to funding techniques?

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false What requirements apply to funding techniques? 205.11 Section 205.11 Money and Finance: Treasury Regulations Relating to Money and Finance... Treasury-State Agreement § 205.11 What requirements apply to funding techniques? (a) A State and a Federal...

  11. 31 CFR 205.10 - How do you document funding techniques?

    Science.gov (United States)

    2010-07-01

    ... techniques? 205.10 Section 205.10 Money and Finance: Treasury Regulations Relating to Money and Finance... Treasury-State Agreement § 205.10 How do you document funding techniques? The Treasury-State agreement must include a concise description for each funding technique that a State will use. The description must...

  12. 7 CFR 1486.205 - What types of funding are available under the program?

    Science.gov (United States)

    2010-01-01

    ... MARKETS PROGRAM Eligibility, Applications, and Funding § 1486.205 What types of funding are available... 7 Agriculture 10 2010-01-01 2010-01-01 false What types of funding are available under the program? 1486.205 Section 1486.205 Agriculture Regulations of the Department of Agriculture (Continued...

  13. 12 CFR 205.18 - Requirements for financial institutions offering payroll card accounts.

    Science.gov (United States)

    2010-01-01

    ... offering payroll card accounts. 205.18 Section 205.18 Banks and Banking FEDERAL RESERVE SYSTEM BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM ELECTRONIC FUND TRANSFERS (REGULATION E) § 205.18 Requirements for financial institutions offering payroll card accounts. (a) Coverage. A financial institution shall comply...

  14. 7 CFR 810.205 - Grades and grade requirements for Two-rowed Malting barley.

    Science.gov (United States)

    2010-01-01

    ... barley. 810.205 Section 810.205 Agriculture Regulations of the Department of Agriculture (Continued... AGRICULTURE OFFICIAL UNITED STATES STANDARDS FOR GRAIN United States Standards for Barley Principles Governing the Application of Standards § 810.205 Grades and grade requirements for Two-rowed Malting barley...

  15. 40 CFR 2.205 - Final confidentiality determination by EPA legal office.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Final confidentiality determination by EPA legal office. 2.205 Section 2.205 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL PUBLIC INFORMATION Confidentiality of Business Information § 2.205 Final confidentiality...

  16. 48 CFR 2131.205-1 - Public relations and advertising costs.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Public relations and advertising costs. 2131.205-1 Section 2131.205-1 Federal Acquisition Regulations System OFFICE OF PERSONNEL... relations and advertising costs. The provisions of FAR 31.205-1 shall be modified to include the following...

  17. 48 CFR 31.205-1 - Public relations and advertising costs.

    Science.gov (United States)

    2010-10-01

    ... advertising costs. 31.205-1 Section 31.205-1 Federal Acquisition Regulations System FEDERAL ACQUISITION... Organizations 31.205-1 Public relations and advertising costs. (a) Public relations means all functions and... such as advertising, customer relations, etc. (b) Advertising means the use of media to promote the...

  18. 48 CFR 231.205-1 - Public relations and advertising costs.

    Science.gov (United States)

    2010-10-01

    ... advertising costs. 231.205-1 Section 231.205-1 Federal Acquisition Regulations System DEFENSE ACQUISITION... PROCEDURES Contracts With Commercial Organizations 231.205-1 Public relations and advertising costs. (e) See... public relations and advertising costs also include monies paid to the Government associated with the...

  19. 49 CFR 1542.205 - Security of the security identification display area (SIDA).

    Science.gov (United States)

    2010-10-01

    ... area (SIDA). 1542.205 Section 1542.205 Transportation Other Regulations Relating to Transportation... AIRPORT SECURITY Operations § 1542.205 Security of the security identification display area (SIDA). (a... one SIDA, as follows: (1) Each secured area must be a SIDA. (2) Each part of the air operations area...

  20. 33 CFR 155.205 - Discharge removal equipment for vessels 400 feet or greater in length.

    Science.gov (United States)

    2010-07-01

    ... vessels 400 feet or greater in length. 155.205 Section 155.205 Navigation and Navigable Waters COAST GUARD... REGULATIONS FOR VESSELS Vessel Equipment § 155.205 Discharge removal equipment for vessels 400 feet or greater in length. (a) Oil tankers and offshore oil barges with an overall length of 400 feet or more must...

  1. Prognostic significance of miR-205 in endometrial cancer.

    Directory of Open Access Journals (Sweden)

    Mihriban Karaayvaz

    Full Text Available microRNAs have emerged as key regulators of gene expression, and their altered expression has been associated with tumorigenesis and tumor progression. Thus, microRNAs have potential as both cancer biomarkers and/or potential novel therapeutic targets. Although accumulating evidence suggests the role of aberrant microRNA expression in endometrial carcinogenesis, there are still limited data available about the prognostic significance of microRNAs in endometrial cancer. The goal of this study is to investigate the prognostic value of selected key microRNAs in endometrial cancer by the analysis of archival formalin-fixed paraffin-embedded tissues.Total RNAs were extracted from 48 paired normal and endometrial tumor specimens using Trizol based approach. The expression of miR-26a, let-7g, miR-21, miR-181b, miR-200c, miR-192, miR-215, miR-200c, and miR-205 were quantified by real time qRT-PCR expression analysis. Targets of the differentially expressed miRNAs were quantified using immunohistochemistry. Statistical analysis was performed by GraphPad Prism 5.0.The expression levels of miR-200c (P<0.0001 and miR-205 (P<0.0001 were significantly increased in endometrial tumors compared to normal tissues. Kaplan-Meier survival analysis revealed that high levels of miR-205 expression were associated with poor patient overall survival (hazard ratio, 0.377; Logrank test, P = 0.028. Furthermore, decreased expression of a miR-205 target PTEN was detected in endometrial cancer tissues compared to normal tissues.miR-205 holds a unique potential as a prognostic biomarker in endometrial cancer.

  2. 7 CFR 205.105 - Allowed and prohibited substances, methods, and ingredients in organic production and handling.

    Science.gov (United States)

    2010-01-01

    ... (specified ingredients or food group(s)),” the product must be produced and handled without the use of: (a) Synthetic substances and ingredients, except as provided in § 205.601 or § 205.603; (b) Nonsynthetic... ingredients in organic production and handling. 205.105 Section 205.105 Agriculture Regulations of the...

  3. A 205GHz Amplifier in 90nm CMOS Technology

    Science.gov (United States)

    2017-03-01

    A 205GHz Amplifier in 90nm CMOS Technology Shahab Ardalan, Senior Member, IEEE Electrical Department, Charles W. Davidson College of Engineering...greater CMOS technologies . Keywords: Amplifier, neutralization, parasitic, Psat, P1dB.   Introduction Millimeter-wave and sub-mm-wave (THz band...amplifier has been implemented and fabricated in 90nm CMOS technology . The proposed amplifier attained a gain of 10.5 dB whilst consuming a dc power

  4. Completion report for Well Cluster ER-20-5

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    The Well Cluster ER-20-5 drilling and completion project was conducted for the US Department of Energy, Nevada Operations Office (DOE/NV), in support of the Nevada Environmental Restoration Project at the Nevada Test Site (NTS) in Nye County, Nevada. Its primary tasks include collecting geological, geophysical, hydrological, and water chemistry data from new and existing wells to define groundwater quality in addition to pathways and rates of groundwater migration. A program of drilling wells near the sites of selected underground nuclear tests (near-field drilling) was implemented to obtain site-specific data about the nature and extent of migration of radionuclides that might have been produced by an underground nuclear explosion. Well Cluster ER-20-5 is the first near-field drilling project initiated at the NTS. This document presents construction data and summarizes the scientific data gathered during the drilling and well-installation phases for all three holes drilled at Well Cluster ER-20-5. Some of this information is preliminary and unprocessed, but was released so that drilling, geotechnical, well design, and completion data could be rapidly disseminated. Additional information about water levels, aquifer testing, and groundwater sampling will be reported after any of this work is performed. Any additional geologic and/or geophysical investigations conducted for this project is described in one or more analysis and interpretation reports. The lithologic and stratigraphic logs, however, are provided in final form.

  5. Navy John Lewis (TAO-205) Class Oiler Shipbuilding Program: Background and Issues for Congress

    Science.gov (United States)

    2016-01-08

    Navy John Lewis (TAO-205) Class Oiler Shipbuilding Program: Background and Issues for Congress Ronald O’Rourke Specialist in Naval Affairs...January 8, 2016 Congressional Research Service 7-5700 www.crs.gov R43546 Navy John Lewis (TAO-205) Class Oiler Shipbuilding Program...Congressional Research Service Summary The John Lewis (TAO-205) class oiler shipbuilding program, previously known as the TAO(X) program, is a program to

  6. Analysis of DC Functions Using CD205-DTR Knock-In Mice.

    Science.gov (United States)

    Fukaya, Tomohiro; Takagi, Hideaki; Uto, Tomofumi; Arimura, Keiichi; Sato, Katsuaki

    2016-01-01

    Dendritic cells (DCs) are essential antigen-presenting cells (APCs) that consist of heterogeneous subsets, mainly classified as conventional DCs (cDCs) and plasmacytoid DCs (pDCs). CD205, an endocytic type I C-type lectin-like molecule that belongs to the mannose receptor family, is mainly expressed on CD8α(+) cDCs. However, it is unclear how CD205(+) cDCs control immune responses in vivo. To evaluate the contribution of CD205(+) cDCs to the immune system, we engineered knock-in (KI) mice that express the diphtheria toxin receptor (DTR) under the control of the Cd205 gene, which allows the selective conditional ablation of CD205(+) cDCs in vivo. Conditional ablation of CD205(+) cDCs impaired the antigen-specific priming of CD8(+) T cells to generate cytotoxic T lymphocytes (CTLs) mediated through cross presentation of soluble antigen. Upon microbial infection, CD205(+) cDCs contributed to the cross priming of CD8(+) T cells for generating antibacterial CTLs to efficiently eliminate pathogens. Here, we provide a protocol for the generation of bone marrow WT/CD205-DT chimeric mice, depletion of CD205(+) DCs and assessment of depletion efficiency, and protocols for in vivo cross presentation assay, CTL generation assay, and antibacterial immunity assay.

  7. 48 CFR 1631.205-72 - FEHBP compensation for personal services.

    Science.gov (United States)

    2010-10-01

    ... REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Commercial Organizations 1631.205-72 FEHBP... employee's right, whether conditional or unconditional, to receive a determinable amount of compensated...

  8. Purification and characterization of a new β-lactamase OXA-205 from Pseudomonas aeruginosa.

    Science.gov (United States)

    Krasauskas, R; Labeikytė, D; Markuckas, A; Povilonis, J; Armalytė, J; Plančiūnienė, R; Kavaliauskas, P; Sužiedėlienė, E

    2015-11-26

    We have identified a novel class 1 integron (1503 bp), named In671 in a clinical Pseudomonas aeruginosa isolate. Integron sequence analysis revealed two gene cassettes, one coding for a new OXA-type β-lactamase designated as OXA-205 and the other coding for the aadB gene that is responsible for aminoglycoside resistance. The 266 amino acid sequence of OXA-205 revealed that this β-lactamase belongs to the Ambler class D showing highest sequence homology to the OXA-2 sub-lineage. Our objective was to purify and characterize β-lactamase OXA-205. Escherichia coli cells were transformed with a plasmid containing cloned bla OXA-205 gene from P. aeruginosa. Purification of overproduced OXA-205 consisted of a single ion-exchange chromatography step. SDS-PAGE and isoelectric focusing were performed to determine the molecular mass and pI, respectively. Size-exclusion chromatography was undertaken to determine the OXA-205 oligomerization state. Substrate hydrolysis reactions were employed to assess enzyme kinetic parameters. Purification of OXA-205 yielded the enzyme with >95 % purity (as verified by SDS-PAGE). Approximate yield of the protein was estimated to be 20 mg per liter of culture. OXA-205 had a pI at 8.1, molecular mass of 26 kDa and a monomeric native structure. Kinetic analysis revealed that OXA-205 hydrolyzed narrow spectrum substrates, including ampicillin, carbenicillin, oxacillin, penicillin G, cefazolin and cefuroxime. Additionally, we observed a substrate inhibition profile towards carbenicillin and oxacillin, but not with ampicillin or penicillin G. Our results also show that OXA-205 conferred unusually high (among class D β-lactamases) resistance towards inhibition by NaCl. OXA-205 can be considered a narrow spectrum monomeric β-lactamase that demonstrates unusually high resistance profile towards inhibition by NaCl.

  9. 9 CFR 205.207 - “Amount” and “County or parish”.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false âAmountâ and âCounty or parishâ. 205.207 Section 205.207 Animals and Animal Products GRAIN INSPECTION, PACKERS AND STOCKYARDS... of such product owned by the person in question is subject to the security interest in question. (c...

  10. 9 CFR 205.211 - Applicability of court decisions under the UCC.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Applicability of court decisions under the UCC. 205.211 Section 205.211 Animals and Animal Products GRAIN INSPECTION, PACKERS AND STOCKYARDS... a person engaged in farming operations takes free of a security interest created by his seller even...

  11. 9 CFR 205.213 - Obligations subject-“person indebted”-“debtor.”

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Obligations subject-âperson indebtedâ-âdebtor.â 205.213 Section 205.213 Animals and Animal Products GRAIN INSPECTION, PACKERS AND STOCKYARDS... to a security interest for another's debt. However the terms “person indebted” and “debtor” in the...

  12. 9 CFR 205.104 - Registration of buyer, commission merchant, or selling agent-minimum information.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Registration of buyer, commission merchant, or selling agent-minimum information. 205.104 Section 205.104 Animals and Animal Products GRAIN... registrant is interested; and (3) If registrant is interested only in such product or products produced or...

  13. 9 CFR 205.210 - Effect of EFS outside State in which filed.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Effect of EFS outside State in which filed. 205.210 Section 205.210 Animals and Animal Products GRAIN INSPECTION, PACKERS AND STOCKYARDS... system subject to the security interest in that product whether or not they know about it, even if they...

  14. 7 CFR 22.205 - Allocation of loan and grant funds.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Allocation of loan and grant funds. 22.205 Section 22.205 Agriculture Office of the Secretary of Agriculture RURAL DEVELOPMENT COORDINATION Roles and... Electrification Administration. (b) During the second half of the fiscal year, the Secretary of Agriculture shall...

  15. 31 CFR 205.26 - What are the requirements for preparing Annual Reports?

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false What are the requirements for preparing Annual Reports? 205.26 Section 205.26 Money and Finance: Treasury Regulations Relating to Money... prescribe that the Annual Report be submitted by electronic means. (b) A State must submit a description and...

  16. 7 CFR 205.640 - Fees and other charges for accreditation.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Fees and other charges for accreditation. 205.640 Section 205.640 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL...

  17. 7 CFR 205.302 - Calculating the percentage of organically produced ingredients.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Calculating the percentage of organically produced ingredients. 205.302 Section 205.302 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT...

  18. 7 CFR 205.207 - Wild-crop harvesting practice standard.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Wild-crop harvesting practice standard. 205.207 Section 205.207 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL...

  19. 7 CFR 205.510 - Annual report, recordkeeping, and renewal of accreditation.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Annual report, recordkeeping, and renewal of accreditation. 205.510 Section 205.510 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT...

  20. 7 CFR 205.622 - Review of approved State organic programs.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Review of approved State organic programs. 205.622 Section 205.622 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL...

  1. 7 CFR 205.604 - Nonsynthetic substances prohibited for use in organic livestock production.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Nonsynthetic substances prohibited for use in organic livestock production. 205.604 Section 205.604 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS...

  2. 7 CFR 205.642 - Fees and other charges for certification.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Fees and other charges for certification. 205.642 Section 205.642 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL...

  3. 7 CFR 205.310 - Agricultural products produced on an exempt or excluded operation.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Agricultural products produced on an exempt or excluded operation. 205.310 Section 205.310 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION AC...

  4. 7 CFR 205.602 - Nonsynthetic substances prohibited for use in organic crop production.

    Science.gov (United States)

    2010-01-01

    ... crop production. 205.602 Section 205.602 Agriculture Regulations of the Department of Agriculture... organic crop production. The following nonsynthetic substances may not be used in organic crop production... AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Administrative The...

  5. 31 CFR 20.205 - What must I include in my drug-free workplace statement?

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false What must I include in my drug-free workplace statement? 20.205 Section 20.205 Money and Finance: Treasury Office of the Secretary of the Treasury GOVERNMENTWIDE REQUIREMENTS FOR DRUG-FREE WORKPLACE (FINANCIAL ASSISTANCE) Requirements for...

  6. 49 CFR 40.205 - How are drug test problems corrected?

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false How are drug test problems corrected? 40.205... WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Problems in Drug Tests § 40.205 How are drug test problems...), you must try to correct the problem promptly, if doing so is practicable. You may conduct another...

  7. 7 CFR 205.204 - Seeds and planting stock practice standard.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Seeds and planting stock practice standard. 205.204... Requirements § 205.204 Seeds and planting stock practice standard. (a) The producer must use organically grown seeds, annual seedlings, and planting stock: Except, That, (1) Nonorganically produced, untreated seeds...

  8. The Ionized Gas in Nearby Galaxies as Traced by the [NII] 122 and 205 um Transitions

    NARCIS (Netherlands)

    Herrera-Camus, Rodrigo; Bolatto, Alberto; Smith, J. D T; Draine, Bruce; Pellegrini, E.; Wolfire, Mark; Croxall, Kevin; Looze, Ilse De; Calzetti, D.; Kennicutt, Robert; Crocker, A.; Armus, Lee; van der werf, p; Sandstrom, Karin; Galametz, Maud; Brandl, B.R.; Groves, Brent; Rigopoulou, D.; Walter, Fabian; Leroy, A.; Boquien, Mederic; Tabatabaei, F. S.; Beirão, Pedro

    2016-01-01

    The [NII] 122 and 205 um transitions are powerful tracers of the ionized gas in the ISM: (1) the [NII] 122/205 line ratio can be used to measure the electron density of the low-excitation, ionized gas, and (2) the intensity of these lines is directly related to the flux of ionizing photons, probing

  9. 31 CFR 538.205 - Prohibited exportation and reexportation of goods, technology, or services to Sudan.

    Science.gov (United States)

    2010-07-01

    ... reexportation of goods, technology, or services to Sudan. 538.205 Section 538.205 Money and Finance: Treasury... goods, technology, or services to Sudan. Except as otherwise authorized, the exportation or reexportation, directly or indirectly, to Sudan of any goods, technology (including technical data, software, or...

  10. 14 CFR 121.205 - Nontransport category airplanes: Landing limitations: Alternate airport.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Nontransport category airplanes: Landing limitations: Alternate airport. 121.205 Section 121.205 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... airport. No person may list an airport as an alternate airport in a dispatch or flight release for a...

  11. 40 CFR Appendix I to Subpart B of... - Appendix I to Subpart B of Part 205

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Appendix I to Subpart B of Part 205 I Appendix I to Subpart B of Part 205 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks Pt...

  12. 40 CFR 205.162-3 - Instructions for maintenance, use, and repair.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Instructions for maintenance, use, and repair. 205.162-3 Section 205.162-3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED...-technical language. (3) The instructions must not be used to secure an unfair competitive advantage. They...

  13. File list: Oth.Brs.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Brs.20.5-Methylcytosine.AllCell mm9 TFs and others 5-Methylcytosine Breast http...://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Brs.20.5-Methylcytosine.AllCell.bed ...

  14. File list: Oth.Oth.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Oth.20.5-Methylcytosine.AllCell hg19 TFs and others 5-Methylcytosine Others htt...p://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Oth.20.5-Methylcytosine.AllCell.bed ...

  15. File list: Oth.Plc.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Plc.20.5-Methylcytosine.AllCell mm9 TFs and others 5-Methylcytosine Placenta ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Plc.20.5-Methylcytosine.AllCell.bed ...

  16. File list: Oth.Utr.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Utr.20.5-Methylcytosine.AllCell mm9 TFs and others 5-Methylcytosine Uterus http...://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Utr.20.5-Methylcytosine.AllCell.bed ...

  17. File list: Oth.PSC.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.20.5-Methylcytosine.AllCell mm9 TFs and others 5-Methylcytosine Pluripotent... stem cell http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.20.5-Methylcytosine.AllCell.bed ...

  18. File list: Oth.Unc.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Unc.20.5-Methylcytosine.AllCell mm9 TFs and others 5-Methylcytosine Unclassifie...d http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Unc.20.5-Methylcytosine.AllCell.bed ...

  19. File list: Oth.Myo.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Myo.20.5-Methylcytosine.AllCell mm9 TFs and others 5-Methylcytosine Muscle http...://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Myo.20.5-Methylcytosine.AllCell.bed ...

  20. File list: Oth.Gon.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Gon.20.5-Methylcytosine.AllCell hg19 TFs and others 5-Methylcytosine Gonad http...://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Gon.20.5-Methylcytosine.AllCell.bed ...

  1. File list: Oth.Prs.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Prs.20.5-Methylcytosine.AllCell hg19 TFs and others 5-Methylcytosine Prostate h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Prs.20.5-Methylcytosine.AllCell.bed ...

  2. File list: Oth.Kid.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  3. File list: Oth.Lng.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  4. File list: Oth.PSC.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  5. File list: Oth.Epd.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  6. File list: Oth.CDV.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  7. File list: Oth.Neu.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  8. File list: Oth.Pan.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Pan.20.5-Methylcytosine.AllCell mm9 TFs and others 5-Methylcytosine Pancreas ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Pan.20.5-Methylcytosine.AllCell.bed ...

  9. File list: Oth.Gon.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  10. File list: Oth.Prs.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  11. File list: Oth.ALL.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  12. File list: Oth.Bon.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  13. File list: Oth.Brs.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  14. File list: Oth.Plc.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  15. File list: Oth.Myo.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  16. 7 CFR 205.201 - Organic production and handling system plan.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Organic production and handling system plan. 205.201... Requirements § 205.201 Organic production and handling system plan. (a) The producer or handler of a production... ingredients or food group(s))” must develop an organic production or handling system plan that is agreed to by...

  17. 33 CFR 263.23 - Small flood control project authority (Section 205).

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Small flood control project..., DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE CONTINUING AUTHORITIES PROGRAMS Flood Control Policy § 263.23 Small flood control project authority (Section 205). (a) Legislative authority. Section 205 of the Flood...

  18. 20 CFR 205.3 - Factors considered in determining employee representative status.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Factors considered in determining employee representative status. 205.3 Section 205.3 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE..., or sleeping car company, directly or indirectly, in its formation, in influencing employees to join...

  19. 31 CFR 205.2 - What definitions apply to this part?

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false What definitions apply to this part? 205.2 Section 205.2 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE RULES AND PROCEDURES FOR...

  20. 31 CFR 205.1 - What Federal assistance programs are covered by this part?

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false What Federal assistance programs are covered by this part? 205.1 Section 205.1 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE RULES AND...

  1. 40 CFR 33.205 - How does an entity become certified by EPA?

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false How does an entity become certified by EPA? 33.205 Section 33.205 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE PARTICIPATION BY DISADVANTAGED BUSINESS ENTERPRISES IN UNITED STATES ENVIRONMENTAL...

  2. 40 CFR 1033.205 - Applying for a certificate of conformity.

    Science.gov (United States)

    2010-07-01

    ... conformity. 1033.205 Section 1033.205 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... Applying for a certificate of conformity. (a) Send the Designated Compliance Officer a complete application for each engine family for which you are requesting a certificate of conformity. (b) (c) You must...

  3. 48 CFR 1631.205-70 - FEHBP public relations and advertising costs.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true FEHBP public relations and advertising costs. 1631.205-70 Section 1631.205-70 Federal Acquisition Regulations System OFFICE OF PERSONNEL... relations and advertising costs. (a) The cost of media messages that are directed at advising current FEHBP...

  4. 45 CFR 205.45 - Federal financial participation in relation to State emergency welfare preparedness.

    Science.gov (United States)

    2010-10-01

    ... emergency welfare preparedness under titles I, X, XIV, XVI (AABD) of the Social Security Act. (d) The cost... 45 Public Welfare 2 2010-10-01 2010-10-01 false Federal financial participation in relation to State emergency welfare preparedness. 205.45 Section 205.45 Public Welfare Regulations Relating to...

  5. Polarized proton collisions at 205 GeV at RHIC.

    Science.gov (United States)

    Bai, M; Roser, T; Ahrens, L; Alekseev, I G; Alessi, J; Beebe-Wang, J; Blaskiewicz, M; Bravar, A; Brennan, J M; Bruno, D; Bunce, G; Courant, E; Drees, A; Fischer, W; Gardner, C; Gill, R; Glenn, J; Haeberli, W; Huang, H; Jinnouchi, O; Kewisch, J; Luccio, A; Luo, Y; Nakagawa, I; Okada, H; Pilat, F; Mackay, W W; Makdisi, Y; Montag, C; Ptitsyn, V; Satogata, T; Stephenson, E; Svirida, D; Tepikian, S; Trbojevic, D; Tsoupas, N; Wise, T; Zelenski, A; Zeno, K; Zhang, S Y

    2006-05-05

    The Brookhaven Relativistic Heavy Ion Collider (RHIC) has been providing collisions of polarized protons at a beam energy of 100 GeV since 2001. Equipped with two full Siberian snakes in each ring, polarization is preserved during acceleration from injection to 100 GeV. However, the intrinsic spin resonances beyond 100 GeV are about a factor of 2 stronger than those below 100 GeV making it important to examine the impact of these strong intrinsic spin resonances on polarization survival and the tolerance for vertical orbit distortions. Polarized protons were first accelerated to the record energy of 205 GeV in RHIC with a significant polarization measured at top energy in 2005. This Letter presents the results and discusses the sensitivity of the polarization survival to orbit distortions.

  6. 49 CFR 385.205 - How can a person who has lost his or her certification be re-certified?

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false How can a person who has lost his or her certification be re-certified? 385.205 Section 385.205 Transportation Other Regulations Relating to... Investigators, and Safety Inspectors § 385.205 How can a person who has lost his or her certification be re...

  7. 5 CFR 352.205b - Authority to return an SES employee to his or her former or successor agency.

    Science.gov (United States)

    2010-01-01

    ... his or her former or successor agency. 352.205b Section 352.205b Administrative Personnel OFFICE OF... Between Executive Agencies During Emergencies § 352.205b Authority to return an SES employee to his or her... under this subpart authorizes the return of the employee to his or her former or successor agency when...

  8. File list: Oth.NoD.20.SuBRACKETLvarBRACKETR205.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.NoD.20.SuBRACKETLvarBRACKETR205.AllCell dm3 TFs and others Su(var)205 No descri...ption http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.NoD.20.SuBRACKETLvarBRACKETR205.AllCell.bed ...

  9. File list: Oth.CeL.20.SuBRACKETLvarBRACKETR205.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.CeL.20.SuBRACKETLvarBRACKETR205.AllCell dm3 TFs and others Su(var)205 Cell line... http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.CeL.20.SuBRACKETLvarBRACKETR205.AllCell.bed ...

  10. File list: Oth.Emb.05.SuBRACKETLvarBRACKETR205.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  11. File list: Oth.Lar.05.SuBRACKETLvarBRACKETR205.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Lar.05.SuBRACKETLvarBRACKETR205.AllCell dm3 TFs and others Su(var)205 Larvae SR...X287659,SRX287660 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.Lar.05.SuBRACKETLvarBRACKETR205.AllCell.bed ...

  12. File list: Oth.NoD.10.SuBRACKETLvarBRACKETR205.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  13. 5 CFR 532.205 - The use of Federal, State, and local minimum wage requirements in determining prevailing rates.

    Science.gov (United States)

    2010-01-01

    ... minimum wage requirements in determining prevailing rates. 532.205 Section 532.205 Administrative... Determinations § 532.205 The use of Federal, State, and local minimum wage requirements in determining prevailing... amended, or (2) The highest State or local minimum wage rate in the local wage area which is applicable to...

  14. 41 CFR 102-41.205 - Do we report all forfeited distilled spirits, wine, and beer to GSA for disposal?

    Science.gov (United States)

    2010-07-01

    ... forfeited distilled spirits, wine, and beer to GSA for disposal? 102-41.205 Section 102-41.205 Public..., and Beer § 102-41.205 Do we report all forfeited distilled spirits, wine, and beer to GSA for disposal? (a) Yes, except do not report distilled spirits, wine, and beer not fit for human consumption or for...

  15. Prognostic and Clinical Significance of miRNA-205 in Endometrioid Endometrial Cancer.

    Directory of Open Access Journals (Sweden)

    Milosz Wilczynski

    Full Text Available Endometrial cancer is one of the most common malignancies of the reproductive female tract, with endometrioid endometrial cancer being the most frequent type. Despite the relatively favourable prognosis in cases of endometrial cancer, there is a necessity to evaluate clinical and prognostic utility of new molecular markers. MiRNAs are small, non-coding RNA molecules that take part in RNA silencing and post-transcriptional regulation of gene expression. Altered expression of miRNAs may be associated with cancer initiation, progression and metastatic capabilities. MiRNA-205 seems to be one of the key regulators of gene expression in endometrial cancer. In this study, we investigated clinical and prognostic role of miRNA-205 in endometrioid endometrial cancer. After total RNA extraction from 100 archival formalin-fixed paraffin-embedded tissues, real-time quantitative RT-PCR was used to define miRNA-205 expression levels. The aim of the study was to evaluate miRNA-205 expression levels in regard to patients' clinical and histopathological features, such as: survival rate, recurrence rate, staging, myometrial invasion, grading and lymph nodes involvement. Higher levels of miRNA-205 expression were observed in tumours with less than half of myometrial invasion and non-advanced cancers. Kaplan-Maier analysis revealed that higher levels of miRNA-205 were associated with better overall survival (p = 0,034. These results indicate potential clinical utility of miRNA-205 as a prognostic marker.

  16. Tunable Narrow Linewidth, Low Noise 2.05 Micron Single Frequency Seeder Laser Project

    Data.gov (United States)

    National Aeronautics and Space Administration — We propose an all-fiber based 2.05-micron single frequency, narrow linewidth seeder laser with 10 nm tuning range and 5GHz frequency modulation for next generation...

  17. Turbulence Models: Shock Boundary Layer Interaction at M=2.05

    Data.gov (United States)

    National Aeronautics and Space Administration — Exp: Shock Boundary Layer Interaction at M=2.05. This web page provides data from experiments that may be useful for the validation of turbulence models. This...

  18. 48 CFR 31.205-17 - Idle facilities and idle capacity costs.

    Science.gov (United States)

    2010-10-01

    ... Organizations 31.205-17 Idle facilities and idle capacity costs. (a) Definitions. As used in this subsection..., production economies, reorganization, termination, or other causes which could not have been reasonably...

  19. Tunable Single Frequency 2.05 Micron Fiber Laser Using New Ho-Doped Fiber Project

    Data.gov (United States)

    National Aeronautics and Space Administration — In this proposal, we propose to demonstrate and build a widely tunable, narrow linewidth, single frequency fiber laser near 2.05 micron by developing an innovative...

  20. 48 CFR 33.205 - Relationship of the Act to Pub. L. 85-804.

    Science.gov (United States)

    2010-10-01

    ... Pub. L. 85-804. 33.205 Section 33.205 Federal Acquisition Regulations System FEDERAL ACQUISITION... Relationship of the Act to Pub. L. 85-804. (a) Requests for relief under Pub. L. 85-804 (50 U.S.C. 1431-1435... available only under Pub. L. 85-804; i.e., legal entitlement to rescission or reformation for mutual mistake...

  1. Immune Response of A Novel ATR-AP205-001 Conjugate Anti-hypertensive Vaccine.

    Science.gov (United States)

    Hu, Xiajun; Deng, Yihuan; Chen, Xiao; Zhou, Yanzhao; Zhang, Hongrong; Wu, Hailang; Yang, Shijun; Chen, Fen; Zhou, Zihua; Wang, Min; Qiu, Zhihua; Liao, Yuhua

    2017-10-03

    We developed a virus-like particle (VLP)-based therapeutic vaccine against angiotensin II receptor type 1, ATR-AP205-001, which could significantly reduce the blood pressure and protect target organs of hypertensive animals. In this study, we focused on the immunological effect and safety of the VLP-based vaccine. By comparing to the depolymerized dimeric vaccine ATR-Dimer-001, we found that ATR-AP205-001 reached subcapsular sinus of lymph node shortly after administration, followed by accumulation on follicle dendritic cells via follicle B cell transportation, while ATR-Dimer-001 vaccine showed no association with FDCs. ATR-AP205-001 vaccine strongly activated dendritic cells, which promoted T cells differentiation to follicular helper T cells. ATR-AP205-001 vaccine induced powerful germinal center reaction, which was translated to a boost of specific antibody production and long-lasting B cell memory, far superior to ATR-Dimer-001 vaccine. Moreover, neither cytotoxic T cells, nor Th1/Th17 cell-mediated inflammation was observed in ATR-AP205-001 vaccine, similar to ATR-Dimer-001 vaccine. We concluded that ATR-AP205-001 vaccine quickly induced potent humoral immunity through collaboration of B cells, follicular dendritic cells and follicular helper T cells, providing an effective and safe intervention for hypertension in the future clinical application.

  2. Granuloma cells in chronic inflammation express CD205 (DEC205) antigen and harbor proliferating T lymphocytes: similarity to antigen-presenting cells.

    Science.gov (United States)

    Ohtani, Haruo

    2013-02-01

    Granulomas are classified as immune or foreign body granulomas. Of these, the immune granulomas, a hallmark of granulomatous inflammation, are closely related to cell-mediated immune responses. The aim of the present study is to characterize immune granuloma cells in 33 patients with granulomatous inflammation focusing on the expression of CD205 (DEC205), a cell surface marker of antigen presenting cells, and their spatial relationship to T cells. CD205 was frequently expressed by immune granuloma cells, in contrast to foreign body granuloma cells that lacked CD205 expression. T cells were not only distributed in a lymphocyte collar around the granuloma, but also present among the granuloma cells (termed 'intra-granuloma T cells'). Intra-granuloma T cells stained positive for Ki-67 (median positivity = 9.4%) by double immunostaining for CD3 and Ki-67. This indicated the presence of proliferative stimuli within the granuloma that could activate the intra-granuloma T cells. The labeling index of Ki-67 in intra-granuloma T cells was significantly higher than that of T cells in the lymphocyte collar (P granulomas and antigen presenting cells. © 2013 The Author. Pathology International © 2013 Japanese Society of Pathology and Wiley Publishing Asia Pty Ltd.

  3. 24 CFR 570.205 - Eligible planning, urban environmental design and policy-planning-management-capacity building...

    Science.gov (United States)

    2010-04-01

    ... environmental design and policy-planning-management-capacity building activities. 570.205 Section 570.205..., urban environmental design and policy-planning-management-capacity building activities. (a) Planning... known or suspected environmental contamination. (5) (6) Policy—planning—management—capacity building...

  4. 24 CFR 1003.205 - Eligible planning, urban environmental design and policy-planning-management-capacity building...

    Science.gov (United States)

    2010-04-01

    ... environmental design and policy-planning-management-capacity building activities. 1003.205 Section 1003.205... planning, urban environmental design and policy-planning-management-capacity building activities. (a... plans, general environmental studies, and strategies and action programs to implement plans, including...

  5. 41 CFR 102-85.205 - What happens if a customer agency continues occupancy after the expiration of an OA?

    Science.gov (United States)

    2010-07-01

    ... assignments. However, provisions are necessary to cover the GSA and customer relationship if an OA expires... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false What happens if a customer agency continues occupancy after the expiration of an OA? 102-85.205 Section 102-85.205 Public...

  6. 48 CFR 31.205-16 - Gains and losses on disposition or impairment of depreciable property or other capital assets.

    Science.gov (United States)

    2010-10-01

    ... disposition or impairment of depreciable property or other capital assets. 31.205-16 Section 31.205-16 Federal... or impairment of depreciable property or other capital assets. (a) Gains and losses from the sale... capital assets have been written down from carrying value to fair value due to impairments, gains or...

  7. 45 CFR 2540.205 - What documentation must I maintain regarding a National Service Criminal History Check for a...

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false What documentation must I maintain regarding a National Service Criminal History Check for a covered position? 2540.205 Section 2540.205 Public Welfare Regulations Relating to Public Welfare (Continued) CORPORATION FOR NATIONAL AND COMMUNITY SERVICE GENERAL ADMINISTRATIVE PROVISIONS Requirements...

  8. File list: Oth.NoD.20.5-Methylcytosine.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.NoD.20.5-Methylcytosine.AllCell mm9 TFs and others 5-Methylcytosine No descript...ion http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.NoD.20.5-Methylcytosine.AllCell.bed ...

  9. 21 CFR 1404.205 - Why is it important if a particular transaction is a covered transaction?

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 9 2010-04-01 2010-04-01 false Why is it important if a particular transaction is a covered transaction? 1404.205 Section 1404.205 Food and Drugs OFFICE OF NATIONAL DRUG CONTROL... important if a particular transaction is a covered transaction? The importance of a covered transaction...

  10. 19 CFR 205.6 - Investigations under section 301(e)(3) of the Trade Act of 1974.

    Science.gov (United States)

    2010-04-01

    ...) INTERNATIONAL TRADE OR OF TAKING RETALIATORY ACTIONS TO OBTAIN THE ELIMINATION OF UNJUSTIFIABLE OR UNREASONABLE FOREIGN ACTS OR POLICIES WHICH RESTRICT U.S. COMMERCE Investigations Concerning the Probable Impact on the... Trade Act of 1974. 205.6 Section 205.6 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION...

  11. miR-205 suppresses the proliferative and migratory capacity of human osteosarcoma MG-63 cells by targeting VEGFA

    Directory of Open Access Journals (Sweden)

    Wang L

    2015-09-01

    Full Text Available Li Wang, Minhong Shan, Fengyi Yang, Yang Liu, Hongxia Qi, Lijuan Zhou, Lirong Qiu, Yanshuang Li Department of Operating Room 1, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People’s Republic of China Background: Osteosarcoma (OS is the most common primary bone malignancy in children and young adults. MiR-205 has been reported to be negatively correlated with the proliferation and metastasis of many types of cancer, while its effects on the malignant phenotype of OS are unclear. Methods: Using TaqMan RT polymerase chain reaction analysis, we firstly explored the expression of miR-205 in a panel of OS cell lines. As the expression of miR-205 was significantly decreased in these cell lines, we sought to compensate for its loss by transfection of exogenous miR-205 mimic into MG-63 cells. To further understand the role of miR-205 in OS, we investigated the effects of miR-205 on the proliferation, migration, and invasion of MG-63 cells, and further explored the mechanisms that might be involved. Results: We found that miR-205 was consistently suppressed in OS cells when compared with the normal human osteoblast (NHOst cell line. Restored expression of miR-205 in the OS (MG-63 cell line significantly inhibited cell proliferation, migration, and invasion. Moreover, bioinformatic prediction suggested that vascular endothelial growth factor A (VEGFA was the target oncogene for miR-205 in OS cells. Further quantitative RT polymerase chain reaction and Western blot assays identified that overexpression of miR-205 suppressed expression of VEGFA mRNA and protein. Restored expression of VEGFA in MG-63 cells previously treated with miR-205 mimic could partially abolish miR-205-mediated suppression of proliferation and invasion of these cells. Conclusion: Collectively, these data suggest that miR-205 might function as a tumor suppressor in OS by, at least partially, targeting VEGFA. Keywords: miR-205, osteosarcoma

  12. Direct inactivation of human immunodeficiency virus type 1 by a novel small-molecule entry inhibitor, DCM205.

    Science.gov (United States)

    Duong, Yen T; Meadows, D Christopher; Srivastava, Indresh K; Gervay-Hague, Jacquelyn; North, Thomas W

    2007-05-01

    With more than 40 million people living with human immunodeficiency virus (HIV), there is an urgent need to develop drugs that can be used in the form of a topical microbicide to prevent infection through sexual transmission. DCM205 is a recently discovered small-molecule inhibitor of HIV type 1 (HIV-1) that is able to directly inactivate HIV-1 in the absence of a cellular target. DCM205 is active against CXCR4-, CCR5-, and dual-tropic laboratory-adapted and primary strains of HIV-1. DCM205 binds to the HIV-1 envelope glycoprotein, and competition studies map the DCM205 binding at or near the V3 loop of gp120. Binding to this site interferes with the soluble CD4 interaction. With its ability to disable the virus particle, DCM205 represents a promising new class of HIV entry inhibitor that can be used as a strategy in the prevention of HIV-1/AIDS.

  13. 7 CFR 718.205 - Substantive change in farming operation, and changes in related legal entities.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 7 2010-01-01 2010-01-01 false Substantive change in farming operation, and changes..., Allotments, Quotas, and Bases § 718.205 Substantive change in farming operation, and changes in related legal... the farming operation; (2) The county committee determines that the primary purpose of the request for...

  14. 20 CFR 628.205 - Governor's coordination and special services plan.

    Science.gov (United States)

    2010-04-01

    ... LABOR PROGRAMS UNDER TITLE II OF THE JOB TRAINING PARTNERSHIP ACT State Planning § 628.205 Governor's... shall address the requirements of section 121(b) of the Act, including a description of the Governor's... Job Opportunities and Basic Skills (JOBS) program; the certification of the implementation of the...

  15. Immunomodulatory activity of biopolymeric fraction RLJ-NE-205 from Picrorhiza kurroa.

    Science.gov (United States)

    Gupta, Amit; Khajuria, Anamika; Singh, Jaswant; Bedi, K L; Satti, N K; Dutt, Prabhu; Suri, K A; Suri, O P; Qazi, G N

    2006-10-01

    In the last three decades, numerous biopolymeric fractions have been isolated from medicinal plants and used as a source of therapeutic agents. The most promising biopharmacological activities of these biopolymers are their immunomodulatory effects. The biopolymeric fraction RLJ-NE-205 was isolated and purified from the rhizomes of Picrorhiza kurroa. We evaluated the effects of biopolymeric fraction RLJ-NE-205 from P. kurroa on the in vivo immune function of the mouse. Balb/c mice were treated with the biopolymeric fraction RLJ-NE-205 (12.5, 25 and 50 mg/kg body weight) for 14 days with sheep red blood cells (SRBC) as an antigen. Haemagglutination antibody (HA) titre, plaque forming cell (PFC) assay, delayed type hypersensitivity (DTH) reaction, phagocytic index, proliferation of lymphocytes, analysis of cytokines in serum and CD4/CD8 population in spleen (determined by flowcytometry) were studied. At the dose of 50 mg/kg, significant increases in the proliferation of lymphocytes (p<0.001) and cytokine levels (IL-4 and IFN-gamma) in serum (p<0.001) were observed. A dose dependent increase was demonstrated in HA titre (p<0.05), DTH (p<0.01), PFC (p<0.05), phagocytic index (p<0.05) and CD4/CD8 (p<0.01) population. This suggests that the biopolymeric fraction RLJ-NE-205 improves the immune system and might be regarded as a biological response modifier.

  16. 12 CFR 268.205 - Employment of aliens; Access to sensitive information.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Employment of aliens; Access to sensitive... Complaints § 268.205 Employment of aliens; Access to sensitive information. (a) Definitions. The definitions...— (i) A citizen or National of the United States, (ii) An alien who: (A) Meets the conditions set forth...

  17. 20 CFR 726.205 - Other forms of endorsement and policies.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Other forms of endorsement and policies. 726... MINE OPERATOR'S INSURANCE Insurance Contracts § 726.205 Other forms of endorsement and policies. Forms of endorsement or policies other than that described in § 726.203 may be entered into by operators to...

  18. 7 CFR 760.205 - Eligible producers, owners, and contract growers.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 7 2010-01-01 2010-01-01 false Eligible producers, owners, and contract growers. 760... Livestock, Honeybees, and Farm-Raised Fish Program § 760.205 Eligible producers, owners, and contract... eligibility rules that may apply to be eligible as a: (1) Livestock owner for the payment with respect to the...

  19. 14 CFR 145.205 - Maintenance, preventive maintenance, and alterations performed for certificate holders under...

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Maintenance, preventive maintenance, and...) SCHOOLS AND OTHER CERTIFICATED AGENCIES REPAIR STATIONS Operating Rules § 145.205 Maintenance, preventive.... (a) A certificated repair station that performs maintenance, preventive maintenance, or alterations...

  20. 40 CFR 81.205 - Zanesville-Cambridge Intrastate Air Quality Control Region.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Zanesville-Cambridge Intrastate Air... Air Quality Control Regions § 81.205 Zanesville-Cambridge Intrastate Air Quality Control Region. The Zanesville-Cambridge Intrastate Air Quality Control Region (Ohio) consists of the territorial area...

  1. Fabrication and characterization of MCC approved testing material: ATM-WV/205 glass

    Energy Technology Data Exchange (ETDEWEB)

    Maupin, G.D.; Bowen, W.M.; Daniel, J.L.

    1988-08-01

    The ATM-WV/205 glass was produced in accordance with PNL's QA Manual for License-Related Programs, MCC technical procedures, and MCC QA Plan that were in effect during the course of this work. The method and procedure to be used in the fabrication and characterization of the ATM-WV/205 glass were specified in two run plans for glass preparation and a characterization plan. The ATM-WV/205 glass meets all specifications. The elemental composition and oxidation state of the glass are within the sponsor's specifications. Visually, the ATM-WV/205 glass bars appear uniformly glassy and generally without exterior features. Microscopic examination and x-ray diffraction revealed low (about 0.5 wt %) concentrations of 3-..mu..m iron chrome spinel crystals and 1-..mu..m ruthenium inclusions scattered randomly throughout the glassy matrix. Closed porosity, with pores ranging in diameter from 20 to 135 ..mu..m, was observed in all samples. 3 refs., 10 figs., 21 tabs.

  2. 46 CFR 108.205 - Wash spaces; toilet spaces; and shower spaces.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Wash spaces; toilet spaces; and shower spaces. 108.205...; toilet spaces; and shower spaces. (a) For the purposes of this section— (1) “Private facility” means a toilet, washing, or shower space that is accessible only from one single or double occupancy sleeping...

  3. 7 CFR 205.102 - Use of the term, “organic.”

    Science.gov (United States)

    2010-01-01

    ..., Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Applicability § 205.102 Use of the term, “organic.” Any agricultural product that is sold, labeled, or represented as “100 percent organic,” “organic,” or “made with organic...

  4. 7 CFR 205.300 - Use of the term, “organic.”

    Science.gov (United States)

    2010-01-01

    ..., Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Labels, Labeling, and Market Information § 205.300 Use of the term, “organic.” (a) The term, “organic,” may only be used on labels and in labeling of raw or processed...

  5. 7 CFR 205.203 - Soil fertility and crop nutrient management practice standard.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Soil fertility and crop nutrient management practice... Requirements § 205.203 Soil fertility and crop nutrient management practice standard. (a) The producer must... nutrients and soil fertility through rotations, cover crops, and the application of plant and animal...

  6. 5 CFR 430.205 - Agency performance appraisal program(s).

    Science.gov (United States)

    2010-01-01

    ... REGULATIONS PERFORMANCE MANAGEMENT Performance Appraisal for General Schedule, Prevailing Rate, and Certain Other Employees § 430.205 Agency performance appraisal program(s). (a) Each agency shall establish at... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Agency performance appraisal program(s...

  7. 50 CFR 216.205 - Measures to ensure availability of species for subsistence uses.

    Science.gov (United States)

    2010-10-01

    ... Operation of Offshore Oil and Gas Facilities in the U.S. Beaufort Sea § 216.205 Measures to ensure... proposed activities and to resolve potential conflicts regarding timing and methods of operation; (b) A description of what measures the applicant has taken and/or will take to ensure that oil development...

  8. 7 CFR 205.206 - Crop pest, weed, and disease management practice standard.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Crop pest, weed, and disease management practice... Requirements § 205.206 Crop pest, weed, and disease management practice standard. (a) The producer must use management practices to prevent crop pests, weeds, and diseases including but not limited to: (1) Crop...

  9. MicroRNA-205 downregulates mixed-lineage-AF4 oncogene expression in acute lymphoblastic leukemia

    Directory of Open Access Journals (Sweden)

    Dou L

    2013-08-01

    Full Text Available Liping Dou,1,* Jingxin Li,1,* Dehua Zheng,2,* Yonghui Li,1 Xiaoning Gao,1 Chengwang Xu,1 Li Gao,1 Lili Wang,1 Li Yu1 1Department of Hematology, Chinese PLA General Hospital, Beijing, People's Republic of China; 2Department of Hepatobiliary Surgery, Organ Transplant Center, Chinese PLA 309th Hospital, Beijing, People's Republic of China*These authors contributed equally to this workAbstract: Myeloid/lymphoid or mixed-lineage AF4 acute lymphoblastic leukemia (MLL-AF4 ALL is a pediatric leukemia that occurs rarely in adults. MLL-AF4 ALL is typically characterized by the presence of chromosomal translocation (t(4;11(q21;q23, leading to expression of MLL-AF4 fusion protein. Although MLL-AF4 fusion protein triggers a molecular pathogenesis and hematological presentations that are unique to leukemias, the precise role of this oncogene in leukemogenesis remains unclear. Previous studies have indicated that microRNAs (miRs might modulate the expression of MLL-AF4 ALL fusion protein, thereby suggesting the involvement of miR in progression or suppression of MLL-AF4 ALL. We have previously demonstrated that miR-205 negatively regulates transcription of an MLL-AF4 luciferase reporter. Here, we report that exogenous expression of miR-205 in MLL-AF4 human cell lines (RS4;11 and MV4-11 inversely regulates the expression of MLL-AF4 at both messenger RNA (mRNA and protein level. Furthermore, miR-205 significantly induced apoptosis in MLL-AF4 cells as evidenced by Annexin V staining using fluorescence-activated cell sorting (FACS analysis. The proliferative capacity of leukemic cells was suppressed by miR-205. The addition of an miR-205 inhibitor was able to restore the observed effects. In conclusion, these findings demonstrate that miR-205 may have potential value as a novel therapeutic agent in the treatment of MLL-AF4 ALL.Keywords: miR-205, MLL-AF4, leukemia, microRNA, oncogene expression, untranslated regions, proliferation

  10. Structural Instability of the Prion Protein upon M205S/R Mutations Revealed by Molecular Dynamics Simulations

    OpenAIRE

    Hirschberger, Thomas; Stork, Martina; Schropp, Bernhard; Winklhofer, Konstanze F.; Tatzelt, Jörg; Tavan, Paul

    2006-01-01

    The point mutations M205S and M205R have been demonstrated to severely disturb the folding and maturation process of the cellular prion protein (PrPC). These disturbances have been interpreted as consequences of mutation-induced structural changes in PrP, which are suggested to involve helix 1 and its attachment to helix 3, because the mutated residue M205 of helix 3 is located at the interface of these two helices. Furthermore, current models of the prion protein scrapie (PrPSc), which is th...

  11. Stimulation of Innate and Adaptive Immunity by Using Filamentous Bacteriophage fd Targeted to DEC-205.

    Science.gov (United States)

    D'Apice, Luciana; Costa, Valerio; Sartorius, Rossella; Trovato, Maria; Aprile, Marianna; De Berardinis, Piergiuseppe

    2015-01-01

    The filamentous bacteriophage fd, codisplaying antigenic determinants and a single chain antibody fragment directed against the dendritic cell receptor DEC-205, is a promising vaccine candidate for its safety and its ability to elicit innate and adaptive immune response in absence of adjuvants. By using a system vaccinology approach based on RNA-Sequencing (RNA-Seq) analysis, we describe a relevant gene modulation in dendritic cells pulsed with anti-DEC-205 bacteriophages fd. RNA-Seq data analysis indicates that the bacteriophage fd virions are sensed as a pathogen by dendritic cells; they activate the danger receptors that trigger an innate immune response and thus confer a strong adjuvanticity that is needed to obtain a long-lasting adaptive immune response.

  12. 7 CFR 3015.205 - General provisions for grants and cooperative agreements with institutions of higher education...

    Science.gov (United States)

    2010-01-01

    ... agreements with institutions of higher education, other nonprofit organizations, and hospitals. 3015.205... General provisions for grants and cooperative agreements with institutions of higher education, other... higher education, other nonprofit organizations and hospitals that the recipient assure and certify...

  13. Compact, CO2-stabilized tuneable laser at 2.05 microns

    DEFF Research Database (Denmark)

    Westergaard, Philip G.; Thomsen, Jan W.; Henriksen, Martin Romme

    2016-01-01

    We demonstrate a compact fibre-based laser system at 2.05 microns stabilized to a CO2 transition using frequency modulation spectroscopy of a gas-filled hollow-core fibre. The laser exhibits an absolute frequency accuracy of 5 MHz, a frequency stability noise floor of better than 7 kHz or 5......×10−11 and is tunable within ±200 MHz from the molecular resonance frequency while retaining roughly this stability and accuracy....

  14. Navy John Lewis (TAO 205) Class Oiler Shipbuilding Program: Background and Issues for Congress

    Science.gov (United States)

    2016-08-18

    fixed price incentive block buy contract for the first six TAO-205s to GD/NASSCO, and the contract for LHA-8 to HII/Ingalls. The Navy was granted... average of about $45 million, compared to costs under the standard or default DOD approach of annual contracting. Issues for Congress for FY2017...years; the first ship in the class will reach that age in 2021. The ships are about 677 feet long and have a full load displacement of

  15. Comparison and characterization of biosorption by Weissella viridescens MYU 205 of periodic group 12 metal ions.

    Science.gov (United States)

    Kinoshita, Hideki; Ohtake, Fumika; Ariga, Yuuki; Kimura, Kazuhiko

    2016-02-01

    Because heavy metals cause various health hazards, we studied biosorption by Weissella viridescens MYU 205. MYU 205 showed high biosorption for Cd (II) and Hg (II) and was low for Zn (II). The Hg (II) biosorption rate was high at about 80%. Different biosorptions were shown for each metal after successive incubation. About 20% of the Zn (II) biosorption was observed after 3 h. Cd (II) biosorption increased in a time-dependent manner until 3 h, then gradually decreased. Hg (II) was immediately sorbed at 79.6 ± 4.7% and decreased at 3 h to 52.9 ± 2.6%, and then gradually increased to 77.8 ± 3.6%. Using heat-killed cells, the rate of biosorption of Zn (II) and Cd (II) decreased whereas Hg (II) tended to increase. The metal resistance was high, that is Zn (II) > Cd (II) > Hg (II); while the affinity was opposite where MYU 205 showed high affinity to Hg (II) and low affinity to Zn (II). Our data shows lactic acid bacteria may be powerful heavy metal sorbents for detoxification. © 2015 Japanese Society of Animal Science.

  16. 9 CFR 205.212 - “Buyer in ordinary course of business” and “security interest.”

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false âBuyer in ordinary course of businessâ and âsecurity interest.â 205.212 Section 205.212 Animals and Animal Products GRAIN INSPECTION, PACKERS... business” and “security interest.” The terms “buyer in ordinary course of business” and “security interest...

  17. miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes

    Energy Technology Data Exchange (ETDEWEB)

    McKenna, Declan J., E-mail: dj.mckenna@ulster.ac.uk [Biomedical Sciences Research Institute, University of Ulster, Coleraine, Co. Derry BT52 1SA (United Kingdom); Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen' s University Belfast, Belfast BT9 7BL (United Kingdom); Patel, Daksha, E-mail: d.patel@qub.ac.uk [Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen' s University Belfast, Belfast BT9 7BL (United Kingdom); McCance, Dennis J., E-mail: d.mccance@qub.ac.uk [Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen' s University Belfast, Belfast BT9 7BL (United Kingdom)

    2014-01-05

    A screen of microRNA (miRNA) expression following differentiation in human foreskin keratinocytes (HFKs) identified changes in several miRNAs, including miR-24 and miR-205. We investigated how expression of Human Papilloma Virus Type-16 (HPV16) onco-proteins E6 and E7 affected expression of miR-24 and miR-205 during proliferation and differentiation of HFKs. We show that the induction of both miR-24 and miR-205 observed during differentiation of HFKs is lost in HFKs expressing E6 and E7. We demonstrate that the effect on miR-205 is due to E7 activity, as miR-205 expression is dependent on pRb expression. Finally, we provide evidence that miR-24 effects in the cell may be due to targeting of cyclin dependent kinase inhibitor p27. In summary, these results indicate that expression of both miR-24 and miR-205 are impacted by E6 and/or E7 expression, which may be one mechanism by which HPV onco-proteins can disrupt the balance between proliferation and differentiation in keratinocytes. - Highlights: • miR-24 and miR-205 are induced during keratinocyte differentiation. • This induction is lost in keratinocytes expressing HPV onco-proteins E6 and E7. • miR-205 is dependent upon pRb expression. • miR-24 targets p27 in cycling keratinocytes.

  18. Formation law and criterion of nebulous macroscopic segregation in ZL205A alloy castings

    Directory of Open Access Journals (Sweden)

    Li Yusheng

    2008-02-01

    Full Text Available The appearance of macroscopic segregation in ZL205A alloy castings bears a super resemblance to the appearance of shrinkage porosity, and the chemical composition of the segregation is Al2Cu whose microstructure is in the form of dentrite or skeleton crystal. According to the characteristic of nebulous segregation, the formation process could be divided into two steps by the eutectic temperature of Al2Cu. Then a criterion for each of the two steps is brought forward on the basis of the shrinkage porosity criterion of low pressure casting.

  19. Impact of signal peptide and transmembrane segments on expression and biochemical properties of a lipase from Bacillus sphaericus 205y.

    Science.gov (United States)

    Masomian, Malihe; Jasni, Azmiza Syawani; Rahman, Raja Noor Zaliha Raja Abd; Salleh, Abu Bakar; Basri, Mahiran

    2017-12-20

    A total of 97 amino acids, considered as the signal peptide and transmembrane segments were removed from 205y lipase gene using polymerase chain reaction technique that abolished the low activity of this enzyme. The mature enzyme was expressed in Escherichia coli using pBAD expression vector, which gave up to a 13-fold increase in lipase activity. The mature 205y lipase (without signal peptide and transmembrane; -SP/TM) was purified to homogeneity using the isoelectric focusing technique with 53% recovery. Removing of the signal peptide and transmembrane segments had resulted in the shift of optimal pH, an increase in optimal temperature and tolerance towards more water-miscible organic solvents as compared to the characteristics of open reading frame (ORF) of 205y lipase. Also, in the presence of 1mM inhibitors, less decrease in the activity of mature 205y lipase was observed compared to the ORF of the enzyme. Protein structure modeling showed that 205y lipase consisted of an α/β hydrolase fold without lid domain. However, the transmembrane segment could effect on the enzyme activity by covering the active site or aggregation the protein. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Sequence-specific determination of protein and peptide concentrations by absorbance at 205 nm.

    Science.gov (United States)

    Anthis, Nicholas J; Clore, G Marius

    2013-06-01

    Quantitative studies in molecular and structural biology generally require accurate and precise determination of protein concentrations, preferably via a method that is both quick and straightforward to perform. The measurement of ultraviolet absorbance at 280 nm has proven especially useful, since the molar absorptivity (extinction coefficient) at 280 nm can be predicted directly from a protein sequence. This method, however, is only applicable to proteins that contain tryptophan or tyrosine residues. Absorbance at 205 nm, among other wavelengths, has been used as an alternative, although generally using absorptivity values that have to be uniquely calibrated for each protein, or otherwise only roughly estimated. Here, we propose and validate a method for predicting the molar absorptivity of a protein or peptide at 205 nm directly from its amino acid sequence, allowing one to accurately determine the concentrations of proteins that do not contain tyrosine or tryptophan residues. This method is simple to implement, requires no calibration, and should be suitable for a wide range of proteins and peptides. © 2013 The Protein Society.

  1. Element segregation behavior of aluminum-copper alloy ZL205A

    Directory of Open Access Journals (Sweden)

    Fan Li

    2014-11-01

    Full Text Available In aluminum-copper alloy, the segregation has a severe bad effect on the alloying degree, strength and corrosion resistance. A deeper understanding of element segregation behavior will have a great significance on the prevention of segregation. In the study, the element segregation behavior of ZL205A aluminum-copper alloy was investigated by examining isothermally solidified samples using scanning electron microscopy and energy dispersive spectroscopy. The calculated results of segregation coefficients show that Cu and Mn are negative segregation elements; while Ti, V and Zr are positive segregation elements. The sequence of element segregation degree from the greatest to the least in ZL205A alloy is Cu, Mn, V, Ti, Zr and Al. The density of residual liquid is expected to increase with a decrease in the quenching temperature ranging from 630 ºC to 550 ºC. The calculated results confirm that the quenching temperature has an insignificant effect on the liquid density; and the variation of density is mainly due to element segregation. Consequently, segregations of Al, Cu and Mn lead to an increase in density, but Ti, V and Zr present the opposite effect. The contribution of each element to the variation of the liquid density was analyzed. The sequence of contributions of alloying elements to the variation of total liquid density is Cu﹥Al﹥Mn﹥V﹥Ti﹥Zr.

  2. Proton-hole excitation in the closed shell nucleus {sup 205}Au

    Energy Technology Data Exchange (ETDEWEB)

    Podolyak, Zs. [Department of Physics, University of Surrey, Guildford GU2 7XH (United Kingdom)], E-mail: z.podolyak@surrey.ac.uk; Farrelly, G.F.; Regan, P.H.; Garnsworthy, A.B.; Steer, S.J. [Department of Physics, University of Surrey, Guildford GU2 7XH (United Kingdom); Gorska, M. [GSI, Planckstrasse 1, D-64291 Darmstadt (Germany); Benlliure, J.; Casarejos, E. [Universidad de Santiago de Compostela, E-15706, Santiago de Compostela (Spain); Pietri, S. [Department of Physics, University of Surrey, Guildford GU2 7XH (United Kingdom); Gerl, J.; Wollersheim, H.J. [GSI, Planckstrasse 1, D-64291 Darmstadt (Germany); Kumar, R. [Inter University Accelerator Centre, New Delhi (India); Molina, F. [Instituto de Fisica Corpuscular, Universidad de Valencia, E-46071 (Spain); Algora, A. [Instituto de Fisica Corpuscular, Universidad de Valencia, E-46071 (Spain); Institute for Nuclear Research, H-4001 Debrecen (Hungary); Alkhomashi, N. [Department of Physics, University of Surrey, Guildford GU2 7XH (United Kingdom); Benzoni, G. [INFN, Universita degli Studi di Milano, I-20133 Milano (Italy); Blazhev, A. [IKP, Universitaet zu Koeln, D-50937 Koeln (Germany); Boutachkov, P. [GSI, Planckstrasse 1, D-64291 Darmstadt (Germany); Bruce, A.M. [School of Environment and Technology, University of Brighton, Brighton BN2 4GJ (United Kingdom); Caceres, L. [GSI, Planckstrasse 1, D-64291 Darmstadt (Germany); Departamento de Fisica Teorica, Universidad Autonoma de Madrid, E-28049 Madrid (Spain)] (and others)

    2009-02-16

    The neutron-rich N=126 nucleus {sup 205}Au has been populated following the relativistic energy projectile fragmentation of E/A=1 GeV{sup 208}Pb, and studied via charged-particle decay spectroscopy. An internal decay with a transition energy of 907(5) keV and a half-life of T{sub 1/2}=6(2) s has been identified through the observation of the corresponding K and L internal conversion electron lines. The 907 keV energy level corresponds to the {pi}h{sub 11/2}{sup -1} proton-hole state and decays both internally into the {pi}d{sub 3/2}{sup -1} ground-state and via {beta} decay into {sup 205}Hg. The obtained data provides information on the evolution of single-proton hole energies which are vital inputs of shell model descriptions for nuclei around the {sup 208}{sub 82}Pb{sub 126} doubly magic core.

  3. Reaction p+p to gamma +anything at 205 GeV and its implications for pi /sup 0/ production

    CERN Document Server

    Charlton, G; Crawley, H B; Derrick, M; Engelmann, R; Fields, T; Glasser, R G; Hyman, L; Jäger, K; Mehtani, U; Min, Ma Z; Musgrave, B; Oren, Y; Rhines, D; Schreiner, P; Voyvodic, L; Walker, R; Whitmore, J; Yuta, H

    1972-01-01

    From observations of photon conversions into electron pairs in the 30- in. hydrogen bubble chamber exposed to a 205-GeV proton beam at the National Accelerator Laboratory, topological and differential cross sections for the inclusive reaction p+p to gamma +anything have been measured. Comparison with data on the same reaction at the CERN intersecting storage rings suggests that pi /sup 0/ production in pp collisions has reached a scaling limit by 205 GeV. We find that for multiplicities up to about 12, the average number of pi /sup 0/'s per inelastic pp collision increases with the number of charged particles produced. (14 refs).

  4. 9 CFR 205.102 - Name of person subjecting a farm product to a security interest, on EFS and master list-format.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Name of person subjecting a farm product to a security interest, on EFS and master list-format. 205.102 Section 205.102 Animals and Animal... of person subjecting a farm product to a security interest, on EFS and master list—format. On an EFS...

  5. 20 CFR 411.205 - What if I disagree with the PM's decision about whether I am making timely progress toward self...

    Science.gov (United States)

    2010-04-01

    ... about whether I am making timely progress toward self-supporting employment? 411.205 Section 411.205... decision to you. We will consider you to be making timely progress toward self-supporting employment until.... If we decide that you are no longer making timely progress toward self-supporting employment, our...

  6. 37 CFR 205.12 - Process served on the Register of Copyrights or an employee in his or her official capacity.

    Science.gov (United States)

    2010-07-01

    ... Register of Copyrights or an employee in his or her official capacity. 205.12 Section 205.12 Patents... her official capacity. (a) Summonses, complaints and all other process directed to the Copyright Office, the Register of Copyrights or any other Copyright Office employee in his or her official capacity...

  7. Cytocompatibility of direct water synthesized cadmium selenide quantum dots in colo-205 cells

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez-Torres, Marcos R. [Universidad Metropolitana, Nanomaterials Science Laboratory, School of Science and Technology (United States); Velez, Christian; Zayas, Beatriz [Universidad Metropolitana, ChemTox Laboratory, School of Environmental Affairs (United States); Rivera, Osvaldo [Universidad Metropolitana, Nanomaterials Science Laboratory, School of Science and Technology (United States); Arslan, Zikri [Jackson State University, Department of Chemistry (United States); Gonzalez-Vega, Maxine N. [Universidad Metropolitana, Nanomaterials Science Laboratory, School of Science and Technology (United States); Diaz-Diestra, Daysi; Beltran-Huarac, Juan; Morell, Gerardo [University of Puerto Rico, Molecular Science Research Center (United States); Primera-Pedrozo, Oliva M., E-mail: oprimera1@suagm.edu [Universidad Metropolitana, Nanomaterials Science Laboratory, School of Science and Technology (United States)

    2015-06-15

    Cadmium selenide quantum dots (CdSe QDs), inorganic semiconducting nanocrystals, are alluring increased attraction due to their highly refined chemistry, availability, and super tunable optical properties suitable for many applications in different research areas, such as photovoltaics, light-emitting devices, environmental sciences, and nanomedicine. Specifically, they are being widely used in bio-imaging in contrast to organic dyes due to their high brightness and improved photo-stability, and their ability to tune their absorption and emission spectra upon changing the crystal size. The production of CdSe QDs is mostly assisted by trioctylphosphine oxide compound, which acts as solvent or solubilizing agent and renders the QDs soluble in organic compounds (such as toluene, chloroform, and hexane) that are highly toxic. To circumvent the toxicity-related factor in CdSe QDs, we report the synthesis of CdSe QDs capped with thioglycolic acid (TGA) in an aqueous medium, and their biocompatibility in colo-205 cancer cells. In this study, the [Cd{sup 2+}]/[TGA] ratio was adjusted to 11:1 and the Se concentration (10 and 15 mM) was monitored in order to evaluate its influence on the optical properties and cytocompatibility. QDs resulted to be quite stable in water (after purification) and RPMI cell medium and no precipitation was observed for long contact times, making them appealing for in vitro experiments. The spectroscopy analysis, advanced electron microscopy, and X-ray diffractometry studies indicate that the final products were successfully formed exhibiting an improved optical response. Colo-205 cells being exposed to different concentrations of TGA-capped CdSe QDs for 12, 24, and 48 h with doses ranging from 0.5 to 2.0 mM show high tolerance reaching cell viabilities as high as 93 %. No evidence of cellular apoptotic pathways was observed as pointed out by our Annexin V assays at higher concentrations. Moreover, confocal microscopy analysis conducted to

  8. 9 CFR 205.208 - Distribution of portions of master list-registration-information to non-registrants on request.

    Science.gov (United States)

    2010-01-01

    ...), DEPARTMENT OF AGRICULTURE CLEAR TITLE-PROTECTION FOR PURCHASERS OF FARM PRODUCTS Interpretive Opinions § 205... registrants, registrants would not be subject to that security interest. These conclusions are based on the... question arises as to the length of time for which a registration is effective, and whether a registrant...

  9. 5 CFR 352.205a - Authority to return employee to his or her former or successor agency.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Authority to return employee to his or... Executive Agencies During Emergencies § 352.205a Authority to return employee to his or her former or... authorizes the return of the employee to his or her former or successor agency without regard to part 351...

  10. 25 CFR 900.205 - To what non-medical-related claims against self-determination contractors does FTCA apply?

    Science.gov (United States)

    2010-04-01

    ... THE INDIAN SELF-DETERMINATION AND EDUCATION ASSISTANCE ACT Federal Tort Claims Act Coverage General Provisions Non-Medical Related Claims § 900.205 To what non-medical-related claims against self-determination... 25 Indians 2 2010-04-01 2010-04-01 false To what non-medical-related claims against self...

  11. 50 CFR 226.205 - Critical habitat for Snake River sockeye salmon, Snake River fall chinook salmon, and Snake River...

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Critical habitat for Snake River sockeye salmon, Snake River fall chinook salmon, and Snake River spring/summer chinook salmon. 226.205 Section... Snake River sockeye salmon, Snake River fall chinook salmon, and Snake River spring/summer chinook...

  12. 76 FR 41838 - Order Approving Adjustment for Inflation of the Dollar Amount Tests in Rule 205-3 Under the...

    Science.gov (United States)

    2011-07-15

    ... COMMISSION Order Approving Adjustment for Inflation of the Dollar Amount Tests in Rule 205-3 Under the... dollar amount thresholds to account for the effects of inflation in 1998.\\3\\ \\3\\ See Exemption To Allow... and every five years thereafter, the Commission shall adjust for inflation the dollar amount...

  13. Coordinated epigenetic repression of the miR-200 family and miR-205 in invasive bladder cancer

    DEFF Research Database (Denmark)

    Wiklund, Erik D; Bramsen, Jesper B; Hulf, Toby

    2011-01-01

    of miRNA expression arrays, qPCR assays and mass spectrometry DNA methylation analyses, we show that the miR-200 and miR-205 loci are specifically silenced and gain promoter hypermethylation and repressive chromatin marks in muscle invasive bladder tumors and undifferentiated bladder cell lines...

  14. 7 CFR 205.303 - Packaged products labeled “100 percent organic” or “organic.”

    Science.gov (United States)

    2010-01-01

    ... (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Labels, Labeling, and Market Information § 205.303 Packaged products labeled “100 percent organic” or “organic.” (a) Agricultural products... product, the following: (1) The term, “100 percent organic” or “organic,” as applicable, to modify the...

  15. 45 CFR 205.58 - Income and eligibility information; specific agreements required between the State agency and the...

    Science.gov (United States)

    2010-10-01

    ...), ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION-PUBLIC... XVI (AABD) of the Social Security Act must provide that, in carrying out the requirements of §§ 205.55...) Identification of all agency officials, by position with authority to request information; (3) Methods and timing...

  16. Developement of an Optimum Interpolation Analysis Method for the CYBER 205

    Science.gov (United States)

    Nestler, M. S.; Woollen, J.; Brin, Y.

    1985-01-01

    A state-of-the-art technique to assimilate the diverse observational database obtained during FGGE, and thus create initial conditions for numerical forecasts is described. The GLA optimum interpolation (OI) analysis method analyzes pressure, winds, and temperature at sea level, mixing ratio at six mandatory pressure levels up to 300 mb, and heights and winds at twelve levels up to 50 mb. Conversion to the CYBER 205 required a major re-write of the Amdahl OI code to take advantage of the CYBER vector processing capabilities. Structured programming methods were used to write the programs and this has resulted in a modular, understandable code. Among the contributors to the increased speed of the CYBER code are a vectorized covariance-calculation routine, an extremely fast matrix equation solver, and an innovative data search and sort technique.

  17. Insight into the structure and the mechanism of the slow proton transfer in the GFP double mutant T203V/S205A.

    Science.gov (United States)

    Wineman-Fisher, Vered; Simkovitch, Ron; Shomer, Shay; Gepshtein, Rinat; Huppert, Dan; Saif, Mari; Kallio, Karen; Remington, S James; Miller, Yifat

    2014-06-21

    Mutations near the fluorescing chromophore of the green fluorescent protein (GFP) have direct effects on the absorption and emission spectra. Some mutants have significant band shifts and most of the mutants exhibit a loss of fluorescence intensity. In this study we continue our investigation of the factors controlling the excited state proton transfer (PT) process of GFP, in particular to study the effects of modifications to the key side chain Ser205 in wt-GFP, proposed to participate in the proton wire. To this aim we combined mutagenesis, X-ray crystallography, steady-state spectroscopy, time-resolved emission spectroscopy and all-atom explicit molecular dynamics (MD) simulations to study the double mutant T203V/S205A. Our results show that while in the previously described GFP double mutant T203V/S205V the PT process does not occur, in the T203V/S205A mutant the PT process does occur, but with a 350 times slower rate than in wild-type GFP (wt-GFP). Furthermore, the kinetic isotope effect in the GFP double mutant T203V/S205A is twice smaller than in the wt-GFP and in the GFP single mutant S205V, which forms a novel PT pathway. On the other hand, the crystal structure of GFP T203V/S205A does not reveal a viable proton transfer pathway. To explain PT in GFP T203V/S205A, we argue on the basis of the MD simulations for an alternative, novel proton-wire pathway which involves the phenol group of the chromophore and water molecules infrequently entering from the bulk. This alternative pathway may explain the dramatically slow PT in the GFP double mutant T203V/S205A compared to wt-GFP.

  18. Downregulation of miR-205 modulates cell susceptibility to oxidative and endoplasmic reticulum stresses in renal tubular cells.

    Directory of Open Access Journals (Sweden)

    Shiyo Muratsu-Ikeda

    Full Text Available BACKGROUND: Oxidative stress and endoplasmic reticulum (ER stress play a crucial role in tubular damage in both acute kidney injury (AKI and chronic kidney disease (CKD. While the pathophysiological contribution of microRNAs (miRNA to renal damage has also been highlighted, the effect of miRNA on renal damage under oxidative and ER stresses conditions remains elusive. METHODS: We assessed changes in miRNA expression in the cultured renal tubular cell line HK-2 under hypoxia-reoxygenation-induced oxidative stress or ER stress using miRNA microarray assay and real-time RT-PCR. The pathophysiological effect of miRNA was evaluated by cell survival rate, intracellular reactive oxygen species (ROS level, and anti-oxidant enzyme expression in miRNA-inhibited HK-2 or miRNA-overexpressed HK-2 under these stress conditions. The target gene of miRNA was identified by 3'-UTR-luciferase assay. RESULTS: We identified 8 and 10 miRNAs whose expression was significantly altered by oxidative and ER stresses, respectively. Among these, expression of miR-205 was markedly decreased in both stress conditions. Functional analysis revealed that decreased miR-205 led to an increase in cell susceptibility to oxidative and ER stresses, and that this increase was associated with the induction of intracellular ROS and suppression of anti-oxidant enzymes. While increased miR-205 by itself made no change in cell growth or morphology, cell viability under oxidative or ER stress conditions was partially restored. Further, miR-205 bound to the 3'-UTR of the prolyl hydroxylase 1 (PHD1/EGLN2 gene and suppressed the transcription level of EGLN2, which modulates both intracellular ROS level and ER stress state. CONCLUSIONS: miR-205 serves a protective role against both oxidative and ER stresses via the suppression of EGLN2 and subsequent decrease in intracellular ROS. miR-205 may represent a novel therapeutic target in AKI and CKD associated with oxidative or ER stress in tubules.

  19. Synthesis of Alkaloid (−)-205B via Stereoselective Reductive Cross-Coupling and Intramolecular [3+2] Annulation

    Science.gov (United States)

    Yang, Dexi; Micalizio, Glenn C.

    2012-01-01

    An asymmetric synthesis of alkaloid (−)-205B, a tricyclic member of the architecturally diverse family of natural products isolated from the skin of neotropical poison frogs, is described that proceeds through two recently developed stereoselective synthetic methods: (1) Ti-mediated allylic alcohol–imine reductive cross-coupling, and (2) intramolecular [3+2] cycloaddition of a glyoxylate-based homoallylic nitrone. The utility of this latter cycloaddition process for the assembly of the stereochemically dense piperidine core of 205B is noteworthy, as this method enables direct [3+2] annulation of an intermediate homoallylic (E)-nitrone via a pathway that is stereochemically unscathed by competitive [3,3] sigmatropic rearrangement processes. Overall, the synthesis is asymmetric, concise, and highly stereoselective – features which point to the potential future utility of these chemical methods in natural product synthesis and medicinal chemistry. PMID:22957796

  20. The Value of Caspase-3 after the Application of Annona muricata Leaf Extract in COLO-205 Colorectal Cancer Cell Line

    Science.gov (United States)

    Syam, Ari Fahrial; Meilany, Sofy; Laksono, Bayu; Prabu, Oryza Gryagus; Bekti, Heri Setiyo; Indrawati, Lili; Makmun, Dadang

    2017-01-01

    Annona muricata, commonly known as soursop, contains annonacin, acetogenin, and polyphenol which are known to have chemopreventive effects on cancer. In this study, we tend to evaluate the apoptosis-inducing effect of soursop (Annona muricata) leaf extract on the colorectal cancer cell line COLO-205 through the activities of caspase-3 which is a marker of cell apoptosis. Cell cultures were incubated with soursop leaf with a concentration of 10 μg/ml and then compared with those of the incubated positive control leucovorin 10 μg/ml and placebo as a negative control. The apoptotic activity of caspase-3 was measured with ELISA. After the administration of each treatment in the colorectal cancer cell line COLO-205, the expression of caspase-3 activity was 1422 ng/ml after incubation with the extract of Annona muricata leaves, 1373 ng/ml after the administration of leucovorin, and 1297 ng/ml in the one with placebo. Annona muricata leaf extract elevated caspase-3 by 1.09 times compared to that of the pure cell line. Annona muricata leaf extract had a higher value of caspase-3 activity than leucovorin and placebo in the COLO-205 colorectal cancer cell line. These results may suggest that Annona muricata leaf extract had anticancer properties by enhancing caspase-3 activity which is a proapoptotic marker. PMID:28487731

  1. The Value of Caspase-3 after the Application of Annona muricata Leaf Extract in COLO-205 Colorectal Cancer Cell Line

    Directory of Open Access Journals (Sweden)

    Murdani Abdullah

    2017-01-01

    Full Text Available Annona muricata, commonly known as soursop, contains annonacin, acetogenin, and polyphenol which are known to have chemopreventive effects on cancer. In this study, we tend to evaluate the apoptosis-inducing effect of soursop (Annona muricata leaf extract on the colorectal cancer cell line COLO-205 through the activities of caspase-3 which is a marker of cell apoptosis. Cell cultures were incubated with soursop leaf with a concentration of 10 μg/ml and then compared with those of the incubated positive control leucovorin 10 μg/ml and placebo as a negative control. The apoptotic activity of caspase-3 was measured with ELISA. After the administration of each treatment in the colorectal cancer cell line COLO-205, the expression of caspase-3 activity was 1422 ng/ml after incubation with the extract of Annona muricata leaves, 1373 ng/ml after the administration of leucovorin, and 1297 ng/ml in the one with placebo. Annona muricata leaf extract elevated caspase-3 by 1.09 times compared to that of the pure cell line. Annona muricata leaf extract had a higher value of caspase-3 activity than leucovorin and placebo in the COLO-205 colorectal cancer cell line. These results may suggest that Annona muricata leaf extract had anticancer properties by enhancing caspase-3 activity which is a proapoptotic marker.

  2. Effects of long noncoding RNA-ROR on tamoxifen resistance of breast cancer cells by regulating microRNA-205.

    Science.gov (United States)

    Zhang, Hong-Yan; Liang, Feng; Zhang, Jian-Wei; Wang, Fei; Wang, Li; Kang, Xi-Gang

    2017-02-01

    To explore how long noncoding RNA-ROR (lncRNA-ROR) affects the tamoxifen resistance of breast cancer cells. Breast epithelial (MCF10A), breast cancer (MCF7), and natural tamoxifen-resistant breast cancer (MDA-MB-231) cell lines were selected, and the relative lncRNA-ROR expressions were detected using quantitative real-time polymerase chain reaction (qRT-PCR). In vitro induction of TR5 cell line was performed. There were six groups: MCF7, MCF7/TR5, MDA-MB-231, MCF7-ROR, MCF7/TR5 ROR-siRNA, and the MDA-MB-231 ROR-siRNA groups. CCK-8 assay was conducted to assess the changes in drug resistance of each group. The expression of the epithelial mesenchymal transition (EMT) marker was detected using Western blotting. Transwell was selected to test the invasive ability of the cells. Expressions of microRNA-205 (miR-205) and ZEB1/2 were detected using qRT-PCR . Compared with the MCF7 cells, the proliferation rates of the MCF7/TR5 and MDA-MB-231 cells were significantly increased. Compared with the MCF7 cells, the MCF7-ROR cells had remarkably higher proliferation rates, down-regulated E-cadherin and miR-205 expressions, as well as increased vimentin, invasive ability, and mRNA expression of ZEB1 and ZEB2. Compared with the MCF7/TR5 and MDA-MB-231 cells, up-regulated E-cadherin and miR-205 expression, down-regulated expression of vimentin, ZEB1, and ZEB2 mRNA, and decreased invasive ability were observed in the MCF7/TR5 ROR-siRNA and MDA-MB-231 ROR-siRNA cells. In MDA-MB-231 cells, down-regulated lncRNA-ROR could inhibit the EMT of breast cancer cells and enhance the sensibility of breast cancer cells to tamoxifen by increasing miR-205 expression and suppressing the expressions of ZEB1 and ZEB2.

  3. Identification of Sare0718 as an alanine-activating adenylation domain in marine actinomycete Salinispora arenicola CNS-205.

    Science.gov (United States)

    Xia, Sisi; Ma, Yanlin; Zhang, Wei; Yang, Yi; Wu, Shaowen; Zhu, Minzhe; Deng, Lingfu; Li, Bing; Liu, Zhonglai; Qi, Chao

    2012-01-01

    Amino acid adenylation domains (A domains) are critical enzymes that dictate the identity of the amino acid building blocks to be incorporated during nonribosomal peptide (NRP) biosynthesis. NRPs represent a large group of valuable natural products that are widely applied in medicine, agriculture, and biochemical research. Salinispora arenicola CNS-205 is a representative strain of the first discovered obligate marine actinomycete genus, whose genome harbors a large number of cryptic secondary metabolite gene clusters. In order to investigate cryptic NRP-related metabolites in S. arenicola CNS-205, we cloned and identified the putative gene sare0718 annotated "amino acid adenylation domain". Firstly, the general features and possible functions of sare0718 were predicted by bioinformatics analysis, which suggested that Sare0718 is a soluble protein with an AMP-binding domain contained in the sequence and its cognate substrate is L-Val. Then, a GST-tagged fusion protein was expressed and purified to further explore the exact adenylation activity of Sare0718 in vitro. By a newly mentioned nonradioactive malachite green colorimetric assay, we found that L-Ala but not L-Val is the actual activated amino acid substrate and the basic kinetic parameters of Sare0718 for it are K(m) = 0.1164±0.0159 (mM), V(max) = 3.1484±0.1278 (µM/min), k(cat) = 12.5936±0.5112 (min(-1)). By revealing the biochemical role of sare0718 gene, we identified an alanine-activating adenylation domain in marine actinomycete Salinispora arenicola CNS-205, which would provide useful information for next isolation and function elucidation of the whole cryptic nonribosomal peptide synthetase (NRPS)-related gene cluster covering Sare0718. And meanwhile, this work also enriched the biochemical data of A domain substrate specificity in newly discovered marine actinomycete NRPS system, which bioinformatics prediction will largely depend on.

  4. Improved absorption cross-sections of oxygen in the wavelength region 205-240 nm of the Herzberg continuum

    Science.gov (United States)

    Yoshino, K.; Cheung, A. S.-C.; Esmond, J. R.; Parkinson, W. H.; Freeman, D. E.

    1988-01-01

    The laboratory values of the Herzberg continuum absorption cross-section of oxygen at room temperature from Cheung et al. (1986) and Jenouvrier et al. (1986) are compared and analyzed. It is found that there is no discrepancy between the absolute values of these two sets of independent measurements. The values are combined in a linear least-squares fit to obtain improved values of the Herzberg continuum cross-section of oxygen at room temperature throughout the wavelength region 205-240 nm. The results are compared with in situ and other laboratory measurements.

  5. Performance of infrared ear and forehead thermometers: a comparative study in 205 febrile and afebrile children.

    Science.gov (United States)

    Hamilton, Patricia A; Marcos, Lorenzo S; Secic, Michelle

    2013-09-01

    This study compared readings from two professional-grade, commercially available infrared (IR) thermometers, the ThermoScan® PRO 4000 prewarmed tip ear thermometer and the Temporal Scanner(™) TAT-5000 temporal artery thermometer. The repeatability and precision of readings from IR thermometers for professional use were questioned in the past, but in recent years, these types of thermometers have been technologically improved, so their ability to replicate standard temperature readings reliably should be re-examined. Febrile and afebrile children were recruited from the emergency department, overflow treatment areas and the paediatric intensive care unit of a large hospital in Argentina. Each child had a randomised sequence of seven temperature readings, including three from the ear, three from the forehead or behind the ear and one reference oral or rectal reading. Temperature readings were taken with the ThermoScan PRO 4000, the Temporal Scanner TAT-5000 and the monitor mode of SureTemp® Plus, a widely used professional-grade contact thermometer, for reference. Of 205 children, 46% were febrile, per reference thermometer readings. While mean ThermoScan PRO 4000 febrile measurements did not differ significantly from reference, mean Temporal Scanner TAT-5000 febrile measurements were significantly lower (by a mean of 0·42 °C) than the reference. Overall bias of the ThermoScan PRO 4000 was significantly lower than that of the Temporal Scanner TAT-5000; repeatability was 1·5 times higher, and overall false-negative rate was about a third that of Temporal Scanner TAT-5000, when compared to the reference. This study indicates that the ThermoScan PRO 4000 provides measurements closer to those of a professional-grade contact thermometer when compared to the Temporal Scanner TAT-5000. The odds of misclassifying a febrile child as non-febrile were about threefold higher with the Temporal Scanner TAT-5000 temporal artery thermometer than with the ThermoScan® PRO 4000

  6. The miR-200 family and miR-205 are repressed by Twist1 and concurrently silenced and DNA hypermethylated in invasive bladder cancer

    DEFF Research Database (Denmark)

    Wiklund, Erik Digman

    MicroRNAs (miRNA) are small non-coding RNAs commonly deregulated in cancer. The miR-200 family (miR-200a, -200b, -200c, -141 and -429) and miR-205 are encoded in three separate genomic loci and are frequently silenced in advanced cancer. The miR-200 family and miR-205 have been implicated in tumo...... demonstrating concerted transcriptional regulation of the three miR-200 loci. Our data also indicate that DNA hypermethylation of the miR-200 family and miR-205 are possible prognostic markers in bladder cancer.  ......MicroRNAs (miRNA) are small non-coding RNAs commonly deregulated in cancer. The miR-200 family (miR-200a, -200b, -200c, -141 and -429) and miR-205 are encoded in three separate genomic loci and are frequently silenced in advanced cancer. The miR-200 family and miR-205 have been implicated in tumour...... invasion and metastasis by negatively regulating the transcriptional repressors of E-cadherin, ZEB1 and ZEB2. Loss of miR-200 expression leads to silencing of E-cadherin, thereby promoting epithelial to mesenchymal transition (EMT) and loss of cell adhesion. However, little is known about...

  7. Final Report for grant entitled "Production of Astatine-211 for U.S. Investigators"

    Energy Technology Data Exchange (ETDEWEB)

    Wilbur, Daniel Scott

    2012-12-12

    Alpha-particle emitting radionuclides hold great promise in the therapy of cancer, but few alpha-emitters are available to investigators to evaluate. Of the alpha-emitters that have properties amenable for use in humans, 211At is of particular interest as it does not have alpha-emitting daughter radionuclides. Thus, there is a high interest in having a source of 211At for sale to investigators in the US. Production of 211At is accomplished on a cyclotron using an alpha-particle beam irradiation of bismuth metal. Unfortunately, there are few cyclotrons available that can produce an alpha particle beam for that production. The University of Washington has a cyclotron, one of three in the U.S., that is currently producing 211At. In the proposed studies, the things necessary for production and shipment of 211At to other investigators will be put into place at UW. Of major importance is the efficient production and isolation of 211At in a form that can be readily used by other investigators. In the studies, production of 211At on the UW cyclotron will be optimized by determining the best beam energy and the highest beam current to maximize 211At production. As it would be very difficult for most investigators to isolate the 211At from the irradiated target, the 211At-isolation process will be optimized and automated to more safely and efficiently obtain the 211At for shipment. Additional tasks to make the 211At available for distribution include obtaining appropriate shipping vials and containers, putting into place the requisite standard operating procedures for Radiation Safety compliance at the levels of 211At activity to be produced / shipped, and working with the Department of Energy, Isotope Development and Production for Research and Applications Program, to take orders, make shipments and be reimbursed for costs of production and shipment.

  8. Production of Astatine-211 at the Duke University Medical Center for its regional distribution

    Energy Technology Data Exchange (ETDEWEB)

    Zalutsky, Michael [Duke University Medical Center, Durham, NC (United States)

    2016-01-01

    Systemic targeted radiation therapy and radioimmunotherapy continue to be important tools in the treatment of certain cancers. Because of their high energy and short path length, alpha particle emitters such as 211At are more effective than either external beam x- ray or in vivo beta radiation in delivering potentially curative doses of radiation. The limited clinical trials that have been conducted to date have yielded encouraging responses in some patients, e.g., malignant brain tumors. In order to escalate the additional necessary research and development in radiochemistry, radiobiology and efficacy evaluation of alpha particle radiotherapeutics, it is universally agreed that access to an affordable, reliable supply of 211At is warranted. In conjunction with the Department of Energy's intent to enhance stable and radioactive isotope availability for research applications, it is the primary objective of this project to improve 211At production and purification capabilities at Duke so that this radionuclide can be supplied to researchers at other institutions throughout the US.The most widely used 211At production method involves the α,2n reaction on Bismuth using a cyclotron with beams ≤ 28 MeV. Yields can be enhanced with use of an internal target that allows for a higher alpha fluence plus efficient heat dissipation in the target. Both of these items are in place at Duke; however, in order to support production for multi-institutional use, irradiation campaigns in excess of 50 µAp and four hours duration will be needed. Further, post-irradiation processing equipment is lacking that will enable the distribution process. Financial support is sought for i) a shielded, ventilated processing/containment hood; ii) development of a post-irradiation target retrieval system; iii) fabrication of a 211At distillation and recovery module and iv) a performance review and, where needed, an enhancement of seven major subsystems that comprise the CS-30 Cyclotron. With these modifications in place, routine production of ≥200 mCi of At-211 should be readily achievable, given our methodological development of At-211 target preparation, internal target irradiation and dry distillation to recover the radionuclide.

  9. A novel anti-frictional multiphase layer produced by plasma nitriding of PVD titanium coated ZL205A aluminum alloy

    Science.gov (United States)

    Lu, C.; Yao, J. W.; Wang, Y. X.; Zhu, Y. D.; Guo, J. H.; Wang, Y.; Fu, H. Y.; Chen, Z. B.; Yan, M. F.

    2018-02-01

    The heat treatment (consisting of solid solution and aging), is integrated with the nitriding process of titanium coated ZL205A aluminum alloy to improve the surface and matrix mechanical properties simultaneously. Two-step duplex treatment is adopted to prepare the gradient multiphase layer on a magnesium-free ZL205A aluminum-copper based alloy. Firstly, pure titanium film is deposited on the aluminum alloy substrate using magnetron sputtering. Secondly, the Ti-coated specimen is nitrided at the solid solution temperature of the substrate alloying elements in a gas mixture of N2 and H2 and aged at 175 °C. The microstructure evolution, microhardness as well as the wear resistance of obtained multiphase layers are investigated by means of scanning electron microscopy (SEM) equipped with energy dispersive X-ray spectrometer (EDS), microhardness tester and pin-on-disc tribometer. The multiphase layer, dominated by TiN0.3 or Al3Ti, is prepared with significantly increased layer depth after duplex treatment. The surface hardness of multiphase layer is remarkably improved from 23.7HV to 457HV. The core matrix hardness is also increased to 65HV after aging. The wear rate of the multiphase layer decreases about 55.22% and 49.28% in comparison with the aged and Ti coated specimens, respectively. The predominant wear mechanism for the multiphase layer is abrasive and oxidation, but severe adhesive wear for the aged and Ti coated specimens.

  10. Delivery of siRNAs to Dendritic Cells Using DEC205-Targeted Lipid Nanoparticles to Inhibit Immune Responses.

    Science.gov (United States)

    Katakowski, Joseph A; Mukherjee, Gayatri; Wilner, Samantha E; Maier, Keith E; Harrison, Michael Travis; DiLorenzo, Teresa P; Levy, Matthew; Palliser, Deborah

    2016-02-01

    Due to their ability to knock down the expression of any gene, siRNAs have been heralded as ideal candidates for treating a wide variety of diseases, including those involving "undruggable" targets. However, the therapeutic potential of siRNAs remains severely limited by a lack of effective delivery vehicles. Recently, lipid nanoparticles (LNPs) containing ionizable cationic lipids have been developed for hepatic siRNA delivery. However, their suitability for delivery to other cell types has not been determined. We have modified LNPs for preferential targeting to dendritic cells (DCs), central regulators of immune responses. To achieve directed delivery, we coated LNPs with a single-chain antibody (scFv; DEC-LNPs), specific to murine DEC205, which is highly expressed on distinct DC subsets. Here we show that injection of siRNAs encapsulated in DEC-LNPs are preferentially delivered to DEC205(+) DCs. Gene knockdown following uptake of DEC-LNPs containing siRNAs specific for the costimulatory molecules CD40, CD80, and CD86 dramatically decreases gene expression levels. We demonstrate the functionality of this knockdown with a mixed lymphocyte response (MLR). Overall, we report that injection of LNPs modified to restrict their uptake to a distinct cell population can confer profound gene knockdown, sufficient to inhibit powerful immune responses like the MLR.

  11. MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells

    Directory of Open Access Journals (Sweden)

    Yamashita Shunichi

    2011-03-01

    Full Text Available Abstract Background Esophageal squamous cell carcinoma (ESCC is often diagnosed at later stages until they are incurable. MicroRNA (miR is a small, non-coding RNA that negatively regulates gene expression mainly via translational repression. Accumulating evidence indicates that deregulation of miR is associated with human malignancies including ESCC. The aim of this study was to identify miR that could be specifically expressed and exert distinct biological actions in ESCC. Methods Total RNA was extracted from ESCC cell lines, OE21 and TE10, and a non-malignant human esophageal squamous cell line, Het-1A, and subjected to microarray analysis. Expression levels of miR that showed significant differences between the 2 ESCC and Het-1A cells based on the comprehensive analysis were analyzed by the quantitative reverse transcriptase (RT-PCR method. Then, functional analyses, including cellular proliferation, apoptosis and Matrigel invasion and the wound healing assay, for the specific miR were conducted. Using ESCC tumor samples and paired surrounding non-cancerous tissue obtained endoscopically, the association with histopathological differentiation was examined with quantitative RT-PCR. Results Based on the miR microarray analysis, there were 14 miRs that showed significant differences (more than 2-fold in expression between the 2 ESCC cells and non-malignant Het-1A. Among the significantly altered miRs, miR-205 expression levels were exclusively higher in 5 ESCC cell lines examined than any other types of malignant cell lines and Het-1A. Thus, miR-205 could be a specific miR in ESCC. Modulation of miR-205 expression by transfection with its precursor or anti-miR-205 inhibitor did not affect ESCC cell proliferation and apoptosis, but miR-205 was found to be involved in cell invasion and migration. Western blot revealed that knockdown of miR-205 expression in ESCC cells substantially enhanced expression of zinc finger E-box binding homeobox 2

  12. 205 nm continuous-wave laser: application to the measurement of the Lamb shift in hydrogen; Laser continu a 205 nm: application a la mesure du deplacement de lamb dans l'hydrogene

    Energy Technology Data Exchange (ETDEWEB)

    Bourzeix, S

    1995-01-15

    The subject of this thesis is the construction of an experimental set-up, and in particular of a tunable continuous-wave laser at 205 nm, for the measurement of the ground state Lamb shift in atomic hydrogen. Chapter 1 deals with the Lamb shift from a historical point of view, and with the interest of its measurement, for metrology and test of quantum electrodynamics. Chapter 2 is devoted to the theory of the hydrogen atom. The principle of the experiment is based on the comparison of two frequencies which are in a ratio of 4: those of the two-photon transitions of 2S-6S or 2S-6D and 1S-3S. Chapter 3 describes the experimental set-up used to measure the 2S-6D transition which is excited by a titanium-sapphire laser at 820 nm. The 205 nm light required to excite the 1S-3S transition is generated by two frequency-doubling of the titanium-sapphire laser, made in non-linear crystals placed in enhancement cavities. Chapter 4 is entirely devoted to the frequency-doubling. After a recall of non-linear optics, the enhancement cavities are described in detail, as well as the results we achieved. At last chapter 5 describes the research for a signal on the 1S-3S transition: the construction of a ground state atomic beam, and the development of the detection system. This work has led to a preliminary measurement of the ground state Lamb shift in atomic hydrogen: L(1S) = 8172.850 (174) MHz whose result is in very good agreement with both the previous measurements and the most recent theoretical results. (author)

  13. 6 CFR 27.205 - Determination that a chemical facility “presents a high level of security risk.”

    Science.gov (United States)

    2010-01-01

    ... 6 Domestic Security 1 2010-01-01 2010-01-01 false Determination that a chemical facility âpresents... SECURITY, OFFICE OF THE SECRETARY CHEMICAL FACILITY ANTI-TERRORISM STANDARDS Chemical Facility Security Program § 27.205 Determination that a chemical facility “presents a high level of security risk.” (a...

  14. 20 CFR 664.205 - How is the “deficient in basic literacy skills” criterion in § 664.200(c)(1) defined and documented?

    Science.gov (United States)

    2010-04-01

    ... INVESTMENT ACT Eligibility for Youth Services § 664.205 How is the “deficient in basic literacy skills... requirements regarding the “deficient in basic literacy skills” criterion in § 664.200(c)(1) may be established... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false How is the âdeficient in basic literacy...

  15. 17 CFR 275.205-2 - Definition of “specified period” over which the asset value of the company or fund under...

    Science.gov (United States)

    2010-04-01

    ... periodâ over which the asset value of the company or fund under management is averaged. 275.205-2 Section... value of the company or fund under management is averaged. (a) For purposes of this rule: (1) Fulcrum... section, the specified period over which the asset value of the company or fund is averaged for the...

  16. Self-phase modulation induced transmission penalty reduction in a 5 Gbit/s FM/AM conversion system experiment over 205 km of standard fiber

    DEFF Research Database (Denmark)

    Jørgensen, Bo Foged; Pedersen, Rune Johan Skullerud; Jørgensen, Carsten Gudmann

    1994-01-01

    The transmission penalty of a standard non-dispersion shifted fiber is experimentally demonstrated to be reduced by self-phase modulation due to the optical Kerr effect. A 2 dB reduction of transmission penalty is achieved in a 5 Gbit/s FM/AM conversion system experiment over 205 km of fiber...

  17. 41 CFR 102-118.205 - May my agency pay a subcontractor or agent functioning as a warehouseman for the TSP providing...

    Science.gov (United States)

    2010-07-01

    ... subcontractor or agent functioning as a warehouseman for the TSP providing service under the bill of lading? 102....205 May my agency pay a subcontractor or agent functioning as a warehouseman for the TSP providing service under the bill of lading? No, your agency may only pay the TSP with whom it has a contract. The...

  18. 7 CFR 205.309 - Agricultural products in other than packaged form at the point of retail sale that are sold...

    Science.gov (United States)

    2010-01-01

    ... the point of retail sale that are sold, labeled, or represented as âmade with organic (specified..., Labeling, and Market Information § 205.309 Agricultural products in other than packaged form at the point of retail sale that are sold, labeled, or represented as “made with organic (specified ingredients or...

  19. 7 CFR 205.308 - Agricultural products in other than packaged form at the point of retail sale that are sold...

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Agricultural products in other than packaged form at the point of retail sale that are sold, labeled, or represented as â100 percent organicâ or âorganic.â... Information § 205.308 Agricultural products in other than packaged form at the point of retail sale that are...

  20. Immunopathology of postprimary tuberculosis: increased T-regulatory cells and DEC-205-positive foamy macrophages in cavitary lesions.

    Science.gov (United States)

    Welsh, Kerry J; Risin, Semyon A; Actor, Jeffrey K; Hunter, Robert L

    2011-01-01

    Postprimary tuberculosis occurs in immunocompetent people infected with Mycobacterium tuberculosis. It is restricted to the lung and accounts for 80% of cases and nearly 100% of transmission. Little is known about the immunopathology of postprimary tuberculosis due to limited availability of specimens. Tissues from 30 autopsy cases of pulmonary tuberculosis were located. Sections of characteristic lesions of caseating granulomas, lipid pneumonia, and cavitary stages of postprimary disease were selected for immunohistochemical studies of macrophages, lymphocytes, endothelial cells, and mycobacterial antigens. A higher percentage of cells in lipid pneumonia (36.1%) and cavitary lesions (27.8%) were positive for the dendritic cell marker DEC-205, compared to granulomas (9.0%, P system to eradicate tuberculosis.

  1. Role of Micro RNA-205 in Promoting Visceral Adiposity of NZ10 Mice with Polygenic Susceptibility for Type 2 Diabetes.

    Science.gov (United States)

    Adi, Nikhil; Adi, Jennipher; Cesar, Liliana; Kurlansky, Paul; Agatston, Arthur; Webster, Keith A

    2015-07-01

    To characterize diet-dependent miRNA profiles and their targets in the visceral adipose of mice with polygenic susceptibility to type 2 diabetes. Six-week NONcNZO10/LtJ (NZ10) and control SWR/J mice were subjected to high protein-fish oil or control diets for 19 weeks and micro-RNA microarray analyses were implemented on visceral adipose RNA. We found that 27 miRNAs were significantly induced and 10 significantly repressed in the VA of obese NZ10 mice compared with controls. 12 selected regulated miRNAs were confirmed by RT-PCR based on the microarray data and we demonstrated that the expression of these miRNAs remained unaltered in the VA of control SWR mice. To assess the possible functional roles of miRNAs in adipogenesis, we also analyzed their expression in 3T3-L1 cells during growth and differentiation. This revealed that suppression of miRNA-205 alone correlated selectively with increased cell proliferation and lipid formation of adipocytes. Diet and genetics control the expression of obesity-regulated miRNAs in the visceral adipose of NZ10 mice.

  2. Cuminaldehyde from Cinnamomum verum Induces Cell Death through Targeting Topoisomerase 1 and 2 in Human Colorectal Adenocarcinoma COLO 205 Cells

    Directory of Open Access Journals (Sweden)

    Kuen-daw Tsai

    2016-05-01

    Full Text Available Cinnamomum verum, also called true cinnamon tree, is employed to make the seasoning cinnamon. Furthermore, the plant has been used as a traditional Chinese herbal medication. We explored the anticancer effect of cuminaldehyde, an ingredient of the cortex of the plant, as well as the molecular biomarkers associated with carcinogenesis in human colorectal adenocarcinoma COLO 205 cells. The results show that cuminaldehyde suppressed growth and induced apoptosis, as proved by depletion of the mitochondrial membrane potential, activation of both caspase-3 and -9, and morphological features of apoptosis. Moreover, cuminaldehyde also led to lysosomal vacuolation with an upregulated volume of acidic compartment and cytotoxicity, together with inhibitions of both topoisomerase I and II activities. Additional study shows that the anticancer activity of cuminaldehyde was observed in the model of nude mice. Our results suggest that the anticancer activity of cuminaldehyde in vitro involved the suppression of cell proliferative markers, topoisomerase I as well as II, together with increase of pro-apoptotic molecules, associated with upregulated lysosomal vacuolation. On the other hand, in vivo, cuminaldehyde diminished the tumor burden that would have a significant clinical impact. Furthermore, similar effects were observed in other tested cell lines. In short, our data suggest that cuminaldehyde could be a drug for chemopreventive or anticancer therapy.

  3. Along the N=126 closed shell: study of $^{205}$Au through its $\\pi h_{11/2}^{-1}$ isomeric decay

    CERN Multimedia

    2002-01-01

    Excited states have been identified in only three of the N=126 closed shell nuclei 'below' $^{208}$Pb, $^{207}$Tl, $^{206}$Hg and very recently $^{204}$Pt. We aim to extend our knowledge of the neutron-rich N=126 nuclei by observing the internal decay of the $\\pi h^{-1}_{11/2}$ excited state in $^{205}$Au, which is expected to be isomeric. In addition, the decay of the analogous states in the N=122 and N=124 $^{201,203}$Au will be studied. The lifetimes of the expected isomeric states are crucial for the success of the experiment, and they are estimated to be in the range of 0.3-20 s. These are long enough to enable the extraction from the source, but shorter than the $\\beta$-decay half-lives. Proton single-particle energies and transition rates will be extracted, providing information about the robustness of the N=126 shell-closure. Three days of beam-time is requested.

  4. The Value of Caspase-3 after the Application ofAnnona muricataLeaf Extract in COLO-205 Colorectal Cancer Cell Line.

    Science.gov (United States)

    Abdullah, Murdani; Syam, Ari Fahrial; Meilany, Sofy; Laksono, Bayu; Prabu, Oryza Gryagus; Bekti, Heri Setiyo; Indrawati, Lili; Makmun, Dadang

    2017-01-01

    Annona muricata , commonly known as soursop, contains annonacin, acetogenin, and polyphenol which are known to have chemopreventive effects on cancer. In this study, we tend to evaluate the apoptosis-inducing effect of soursop ( Annona muricata ) leaf extract on the colorectal cancer cell line COLO-205 through the activities of caspase-3 which is a marker of cell apoptosis. Cell cultures were incubated with soursop leaf with a concentration of 10  μ g/ml and then compared with those of the incubated positive control leucovorin 10  μ g/ml and placebo as a negative control. The apoptotic activity of caspase-3 was measured with ELISA. After the administration of each treatment in the colorectal cancer cell line COLO-205, the expression of caspase-3 activity was 1422 ng/ml after incubation with the extract of Annona muricata leaves, 1373 ng/ml after the administration of leucovorin, and 1297 ng/ml in the one with placebo. Annona muricata leaf extract elevated caspase-3 by 1.09 times compared to that of the pure cell line. Annona muricata leaf extract had a higher value of caspase-3 activity than leucovorin and placebo in the COLO-205 colorectal cancer cell line. These results may suggest that Annona muricata leaf extract had anticancer properties by enhancing caspase-3 activity which is a proapoptotic marker.

  5. Structure of putative CutA1 from Homo sapiens determined at 2.05 Å resolution

    Energy Technology Data Exchange (ETDEWEB)

    Bagautdinov, Bagautdin, E-mail: bagautdi@spring8.or.jp; Matsuura, Yoshinori; Bagautdinova, Svetlana; Kunishima, Naoki; Yutani, Katsuhide [Protein Structure Analysis Team, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148 (Japan)

    2008-05-01

    The X-ray structure of human CutA1 was solved in space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 68.69, b = 88.84, c = 125.33 Å and six molecules per asymmetric unit. The structure of human brain CutA1 (HsCutA1) has been determined using diffraction data to 2.05 Å resolution. HsCutA1 has been implicated in the anchoring of acetylcholinesterase in neuronal cell membranes, while its bacterial homologue Escherichia coli CutA1 is involved in copper tolerance. Additionally, the structure of HsCutA1 bears similarity to that of the signal transduction protein PII, which is involved in regulation of nitrogen metabolism. Although several crystal structures of CutA1 from various sources with different rotation angles and degrees of interaction between trimer interfaces have been reported, the specific functional role of CutA1 is still unclear. In this study, the X-ray structure of HsCutA1 was determined in space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 68.69, b = 88.84, c = 125.33 Å and six molecules per asymmetric unit. HsCutA1 is a trimeric molecule with intertwined antiparallel β-strands; each subunit has a molecular weight of 14.6 kDa and contains 135 amino-acid residues. In order to obtain clues to the possible function of HsCutA1, its crystal structure was compared with those of other CutA1 and PII proteins.

  6. Anthocyanins extracted from Chinese blueberry (Vaccinium uliginosum L.) and its anticancer effects on DLD-1 and COLO205 cells.

    Science.gov (United States)

    Zu, Xiao-yan; Zhang, Zhen-ya; Zhang, Xiao-wen; Yoshioka, Masahiro; Yang, Ying-nan; Li, Ji

    2010-10-01

    Vaccinium uliginosum L. is a type of blueberry found in the Chinese Changbai Mountains. We extracted Vaccinium uliginosum Anthocyanins (A(V.uli)) to investigate its bioactivity on suppressing cancer cells. A(V.uli) was extracted under different conditions of temperature (10°C - 35°C), pH 1.0 - 3.0, and diatomaceous earth (1.0 g - 3.0 g), followed by a HPLC analysis for the determination of the ingredients. Its anticancer bioactivities on human colon and colorectal cancer cells (DLD-1 and COLO205) were compared with those on Lonicera caerulea Anthocyanins (A(L.cae)) and Vaccinium myrtillus Anthocyanins (A(V.myr)), using cell viability assays, DNA electrophoresis and nuclear morphology assays. The optimum process of A(V.uli) extraction involved conditions of temperature 20°C, pH 2.0, and diatomaceous earth 1.0 g/50 g of fruit weight. A(V.uli) contained 5 main components: delphinidin (40.70 ± 1.72)%, cyanidin (3.40 ± 0.68)%, petunidin (17.70 ± 0.54)%, peonidin (2.90 ± 0.63)% and malvidin (35.50 ± 1.11)%. The malvidin percentage was significantly higher (P < 0.05) than it in A(V.myr). A(V.uli) complied with a dose-dependent repression of cancer cell proliferation with an IC(50) (50% inhibitory concentration) value of 50 µg/ml, and showed greater anticancer efficiency than A(L.cae) and A(V.myr) under the same cell treatment conditions. These observations were further supported by the results of nuclear assays. The extraction protocol and conditions we used were effective for anthocyanin extraction. A(V.uli) could be a feasible practical research tool and a promising therapeutic source to suppress human colon or colorectal cancers.

  7. miRNA array analysis determines miR-205 is overexpressed in head and neck squamous cell carcinoma and enhances cellular proliferation

    Directory of Open Access Journals (Sweden)

    Howard JD

    2013-08-01

    Full Text Available MicroRNAs (miRNAs play a critical role in cell cycle and pro-survival signal regulation. Consequently, their deregulation can enhance tumorigenesis and cancer progression. In the current investigation, we determined whether cancer- or human papillomavirus (HPV-specific miRNA deregulation could further elucidate signal transduction events unique to head and neck squamous cell carcinoma (HNSCC. Twenty-nine newly diagnosed HNSCC tumors (HPV-positive: 14, HPV-negative: 15 and four normal mucosa samples were analyzed for global miRNA expression. Differential miRNA expression analysis concluded HNSCC is characterized by a general upregulation of miRNAs compared to normal mucosa. Additionally, miR-449a and miR-129-3p were statistically significant miRNAs differentially expressed between HPV-positive and HPV-negative HNSCC. The upregulation of miR-449a was also validated within an independent dataset obtained from TCGA containing 279 HNSCCs and 39 normal adjacent mucosa samples. To gain a better understanding of miRNA-mediated cell cycle deregulation in HNSCC, we functionally evaluated miR-205, a transcript upregulated in our cancer-specific analysis and a putative regulator of E2F1. Modulation of miR-205 with a miRNA mimic and inhibitor revealed miR-205 is capable of regulating E2F1 expression in HNSCC and overexpression of this transcript enhances proliferation. This study demonstrates miRNA expression is highly deregulated in HNSCC and functional evaluations of these miRNAs may reveal novel HPV context dependent mechanisms in this disease.

  8. Relevance of MicroRNA200 Family and MicroRNA205 for Epithelial to Mesenchymal Transition and Clinical Outcome in Biliary Tract Cancer Patients

    Directory of Open Access Journals (Sweden)

    Romana Urbas

    2016-12-01

    Full Text Available Extensive stromal interaction is one reason for the dismal outcome of biliary tract cancer (BTC patients. Epithelial to mesenchymal transition (EMT is involved in tumor invasion and metastasis and is partly regulated by microRNAs (miRs. This study explores the expression of anti-EMT miR200 family (miR141, −200a/b/c, −429 and miR205 as well as the EMT-related proteins E-cadherin and vimentin in a panel of BTC cell lines and clinical specimens by quantitative real-time polymerase chain reaction, Western blot and immunohistochemistry, respectively. MicroRNA expression was correlated to (i the expression patterns of E-cadherin and vimentin; (ii clinicopathological characteristics; and (iii survival data. MicroRNA-200 family and miR205 were expressed in all BTC cells and clinical specimens. E-cadherin and vimentin showed a mutually exclusive expression pattern in both, in vitro and in vivo. Expression of miR200 family members positively correlated with E-cadherin and negatively with vimentin expression in BTC cells and specimens. High expression of miR200 family members (but not miR205 and E-cadherin was associated with longer survival, while low miR200 family and high vimentin expression was a predictor of unfavorable survival. Overall, the current study demonstrates the relevance of the miR200 family in EMT of BTC tumors and suggests these miRs as predictors for positive outcome.

  9. Scientific opinion of Flavouring Group Evaluation 205 Revision 1 (FGE.205Rev1): consideration of genotoxicity data on representatives for 13 α,β-unsaturated aliphatic ketones with terminal double bonds and precursors from chemical subgroup 1.2.2 of FGE.19

    DEFF Research Database (Denmark)

    Beltoft, Vibe Meister; Nørby, Karin Kristiane

    of the representative substances, pent-1-en-3-one, in an in vivo Comet assay on the first site of contact (e.g. the stomach or duodenum) and on the liver. The Industry has now submitted new data, a combined micronucleus and Comet assay for pent-1-en-3-one, with scoring in the liver and duodenum, and an Ames test...... and a Comet assay with scoring in the liver for oct-1-en-3-one. Based on these new data, the Panel concluded that the concern for a genotoxic potential could be ruled out for the two representative substances and accordingly for the remaining 11 substances in FGE.205Rev1. The 13 substances in FGE.205Rev1 can...

  10. Comment on “Mathematical modeling of unicellular microalgae and cyanobacteria metabolism for biofuel production” by Baroukh et al. [Curr Opin Biotechnol. 2015, 33:198–205

    Energy Technology Data Exchange (ETDEWEB)

    Song, Hyun-Seob; Ramkrishna, Doraiswami

    2016-04-01

    This letter concerns an article recently published in Current Opinion in Biotechnology by Baroukh et al. entitled "Mathematical modeling of unicellular microalgae and cyanobacteria metabolism for biofuel production" (2015 Mar 26; 33:198-205). The issue we wish to bring to light is the authors’ claim that dynamic metabolic models including Hybrid Cybernetic Model (HCM) (Kim et al., 2008; Song et al., 2009) and Lumped HCM (L-HCM) (Song and Ramkrishna, 2010; 2011) are based on the balanced growth hypothesis so that they are unable to simulate the accumulation of intracellular metabolites. This is a misrepresentation of these models due to the following reasons.

  11. Retrospective analysis of prognostic factors in 205 patients with laryngeal squamous cell carcinoma who underwent surgical treatment.

    Directory of Open Access Journals (Sweden)

    Si-Yi Zhang

    Full Text Available OBJECTIVES: To investigate the most important factors affecting the prognosis of the patients with squamous cell carcinoma (SCC of the larynx. METHODS: Based on the clinical and follow-up data, 205 patients with SCC of the larynx receiving total laryngectomy, partial laryngectomy, or CO2 laser surgery in GuangDong General Hospital were retrospectively analyzed. A survival analysis was performed by the Kaplan-Meier method and a multivariable analysis of prognostic factors was carried out using the Cox proportional hazard model. RESULTS: Subtypes of carcinoma included 69.8% glottic and 30.2% supraglottic. Most patients were in N0 stage (77.6%, and 22.4% patients were in N1∼N3 stage. Over half of the patients were in T1∼T2 stage (55.1%, 20.0% in T3, and 24.9% in T4. Mean follow-up duration was 49.2 months. The survival rates 1, 2, and 3 years after the surgery were 99.0%, 91.7%, and 81.5%, respectively. The survival rate for those patients with clinical stage IV was significantly lower than for those with clinical stage I and II (p<0.001 and p = 0.013, respectively. The disease-free progression rates 1, 2, and 3 years after the surgery were 83.9%, 74.6%, and 71.2%, respectively. Futhermore, those patients with a Charlson score of 1 to 2 and ≥3 had higher risk of mortality than those with a Charlson score of 0 (hazard ratios of 1.8 and 2.41 p = 0.042 and p = 0.008. Multivariable analysis revealed that clinical stage, surgical margin, and comorbidity were significantly associated with both mortality and disease-free progression. CONCLUSION: The surgical resection margin, clinical stage, and comorbidity were independent factors affecting the laryngeal cancer prognosis. The survival rates were lower for patients with advanced laryngeal cancer, positive surgical margins, or severe comorbidity, suggesting the importance of early diagnosis, early treatment, negative surgical margins, and conditions of comorbidity.

  12. ORIGINAL ARTICLE 205

    African Journals Online (AJOL)

    boaz

    role in causing various ailments such as asthma, cystic fibrosis and invasive aspergillosis cannot be overemphasized [1-3]. These organisms has since been indicted in ..... exchange of DNA in the rice blast fungus; challenges its exclusive clonality. Phytopathology.1997; 87(3):284–294. 27.Souza-Paccola EA, Fa´varo LCL, ...

  13. 205 ORIGINAL ARTICLE

    African Journals Online (AJOL)

    boaz

    ABSTRACT. Noroviruses (NoV) are identified as the major cause of epidemic and sporadic acute gastroenteritis. Controlling the spread of the disease needs early recognition of NoV. This study investigated the contribution of norovirus to sporadic cases of pediatric gastroenteritis in Zagazig University Hospitals and studied ...

  14. High resolution diffraction studies with synchrotron radiation on the structure of Li{sub 0.95}Mn{sub 2.05}O{sub 4} spinel

    Energy Technology Data Exchange (ETDEWEB)

    Nowicki, W. [Laboratory of Magnetochemistry, Faculty of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, PL-60780 Poznan (Poland)]. E-mail: waldek@amu.edu.pl; Darul, J. [Laboratory of Magnetochemistry, Faculty of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, PL-60780 Poznan (Poland); Piszora, P. [Laboratory of Magnetochemistry, Faculty of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, PL-60780 Poznan (Poland); Baehtz, C. [Institute of Materials Science, Darmstadt University of Technology, Petersenstr. 32, D-64287 Darmstadt (Germany); Wolska, E. [Laboratory of Magnetochemistry, Faculty of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, PL-60780 Poznan (Poland)

    2005-09-29

    Investigations of the structure transformations of lithium deficient Li{sub 0.95}Mn{sub 2.05}O{sub 4}, in the temperature range of 10-1173 K, have been undertaken with X-ray powder diffraction, using synchrotron radiation, at the HASYLAB high-resolution diffractometer (beamline B2). Single phase spinel-like oxide was obtained by a solid state reaction between {alpha}-Mn{sub 2}O{sub 3} and Li{sub 2}CO{sub 3} at 1073 K, followed by rapid quenching of the sample in the solid CO{sub 2}. At the room temperature, the lithium deficient sample (with x = 0.95 in Li {sub x}Mn{sub 3-x}O{sub 4}) shows a tetragonally distorted spinel lattice, with c/a = 0.98. No phase transition has been observed when temperature decreased and the sample remained tetragonal in the range 300-10 K. A reversible phase transition appears, however, during very mild heating of Li{sub 0.95}Mn{sub 2.05}O{sub 4} above the room temperature. The tetragonal spinel structure (F4{sub 1}/ddm) undergoes a transition into cubic form (Fd3m) at 393 K. A thermal treatment above 573 K causes a partial decomposition of the sample, resulting in a formation of a nearly stoichiometric LiMn{sub 2}O{sub 4}, with the admixture of manganese oxides.

  15. Antigen delivery by filamentous bacteriophage fd displaying an anti-DEC-205 single-chain variable fragment confers adjuvanticity by triggering a TLR9-mediated immune response.

    Science.gov (United States)

    Sartorius, Rossella; D'Apice, Luciana; Trovato, Maria; Cuccaro, Fausta; Costa, Valerio; De Leo, Maria Giovanna; Marzullo, Vincenzo Manuel; Biondo, Carmelo; D'Auria, Sabato; De Matteis, Maria Antonietta; Ciccodicola, Alfredo; De Berardinis, Piergiuseppe

    2015-07-01

    Filamentous bacteriophage fd particles delivering antigenic determinants via DEC-205 (fdsc-αDEC) represent a powerful delivery system that induces CD8(+) T-cell responses even when administered in the absence of adjuvants or maturation stimuli for dendritic cells. In order to investigate the mechanisms of this activity, RNA-Sequencing of fd-pulsed dendritic cells was performed. A significant differential expression of genes involved in innate immunity, co-stimulation and cytokine production was observed. In agreement with these findings, we demonstrate that induction of proinflammatory cytokines and type I interferon by fdsc-αDEC was MYD88 mediated and TLR9 dependent. We also found that fdsc-αDEC is delivered into LAMP-1-positive compartments and co-localizes with TLR9. Thus, phage particles containing a single-strand DNA genome rich in CpG motifs delivered via DEC-205 are able to intercept and trigger the active TLR9 innate immune receptor into late endosome/lysosomes and to enhance the immunogenicity of the displayed antigenic determinants. These findings make fd bacteriophage a valuable tool for immunization without administering exogenous adjuvants. © 2015 The Authors. Published under the terms of the CC BY 4.0 license.

  16. Evaluation of Novel Wet Chemistry Separation and Purification Methods to Facilitate Automation of Astatine-­211 Isolation

    Energy Technology Data Exchange (ETDEWEB)

    Wilbur, Daniel Scott [Univ. of Washington, Seattle, WA (United States)

    2016-07-19

    This research is a collaborative effort between the research groups of the PIs, Dr. D. Scott Wilbur in the Department of Radiation Oncology at the University of Washington (UW) and Matthew O’Hara at the Pacific Northwest National Laboratory (PNNL). In this report only those studies conducted at UW and the budget information from UW will be reported. A separate progress and financial report will be provided by PNNL. This final report outlines the experiments (Tasks) conducted and results obtained at UW from July 1, 2013 thru June 30, 2016 (2-­year project with 1 year no-­cost extension). The report divides the information on the experiments and results obtained into the 5 specific objectives of the research efforts and the Tasks within those objectives. This format is used so that it is easy to see what has been accomplished in each area. A brief summary of the major findings from the studies is provided below. Summary of Major Findings from Research/Training Activities at UW: Anion and cation exchange columns did not provide adequate 211At capture and/or extraction results under conditions studied to warrant further evaluation; PEG-­Merrifield resins containing mPEG350, mPEG750, mPEG2000 and mPEG5000 were synthesized and evaluated; All of the mPEG resins with different sized mPEG moieties conjugated gave similar 211At capture (>95%) from 8M HCl solutions and release with conc. NH4OH (~50-­80%), but very low quantities were released when NaOH was used as an eluent; Capture and release of 211At when loading [211At]astatate appeared to be similar to that of [211At]astatide on PEG columns, but further studies need to be conducted to confirm that; Capture of 211At on PEG columns was lower (e.g. 80-­90%) from solutions of 8M HNO3, but higher capture rates (e.g. 99%) can be obtained when 10M HNO3 is mixed with an equal quantity of 8M HCl; Addition of reductants to the 211At solutions did not appear to change the percent capture, but may have an effect on the % extracted; There was some indication that the PEG-­Merrifield resins could be saturated (perhaps with Bi) resulting in lower capture percentages, but more studies need to be done to confirm that; A target dissolution chamber, designed and built at PNNL, works well with syringe pumps so it can be used in an automated system; Preliminary semi-­automated 211At isolation studies have been conducted with full-scale target dissolution and 211At isolation using a PEG column on the Hamilton automated system gave low overall recoveries, but HNO3 was used (rather than HCl) for loading the 211At and flow rates were not optimized; Results obtained using PEG columns are high enough to warrant further development on a fully automated system; Results obtained also indicate that additional studies are warranted to evaluate other types of columns for 211At separation from bismuth, which allow use of HNO3/HCl mixtures for loading and NaOH for eluting 211At. Such a column could greatly simplify the overall isolation process and make it easier to automate.

  17. MicroRNA profiling in prostate cancer--the diagnostic potential of urinary miR-205 and miR-214.

    Directory of Open Access Journals (Sweden)

    Anvesha Srivastava

    Full Text Available Prostate cancer (PCa is the most common type of cancer in men in the United States, which disproportionately affects African American descents. While metastasis is the most common cause of death among PCa patients, no specific markers have been assigned to severity and ethnic biasness of the disease. MicroRNAs represent a promising new class of biomarkers owing to their inherent stability and resilience. In the present study, we investigated potential miRNAs that can be used as biomarkers and/or therapeutic targets and can provide insight into the severity and ethnic biasness of PCa. PCR array was performed in FFPE PCa tissues (5 Caucasian American and 5 African American and selected differentially expressed miRNAs were validated by qRT-PCR, in 40 (15 CA and 25 AA paired PCa and adjacent normal tissues. Significantly deregulated miRNAs were also analyzed in urine samples to explore their potential as non-invasive biomarker for PCa. Out of 8 miRNAs selected for validation from PCR array data, miR-205 (p<0.0001, mir-214 (p<0.0001, miR-221(p<0.001 and miR-99b (p<0.0001 were significantly downregulated in PCa tissues. ROC curve shows that all four miRNAs successfully discriminated between PCa and adjacent normal tissues. MiR-99b showed significant down regulation (p<0.01 in AA PCa tissues as compared to CA PCa tissues and might be related to the aggressiveness associated with AA population. In urine, miR-205 (p<0.05 and miR-214 (p<0.05 were significantly downregulated in PCa patients and can discriminate PCa patients from healthy individuals with 89% sensitivity and 80% specificity. In conclusion, present study showed that miR-205 and miR-214 are downregulated in PCa and may serve as potential non-invasive molecular biomarker for PCa.

  18. Tendência genética para pesos padronizados aos 205, 365 e 550 dias de idade de bovinos Nelore da região norte do Brasil=Genetic trend for adjusted weight at 205, 365 and 550 days of age of Nellore cattle from Northern Brazil

    Directory of Open Access Journals (Sweden)

    Tânia Vasconcelos Cavalcante

    2012-01-01

    Full Text Available Pesquisas efetivas que avaliam a eficiência dos rebanhos bovinos na região Norte do Brasil são insipientes. Por isso, objetivou-se avaliar as mudanças genéticas aditivas diretas e maternais dos pesos padronizados para 205 (P205, 365 (P365 e 550 (P550 dias de idade, de animais da raça Nelore, criados a pasto, nascidos entre 1997 e 2007 na região Norte do Brasil. As estimativas dos componentes de (covariâncias utilizadas no cálculo dos valores genéticos foram obtidas pelo método de máxima verossimilhança restrita livre de derivadas (REML, usando o aplicativo MTDFREML. As tendências genéticas dos efeitos genéticos direto e maternal foram estimadas pela regressão, ponderada, das médias anuais dos valores genéticos dos animais. As estimativas das herdabilidade para P205, P365 e P550 foram 0,33; 0,51 e 0,41, com ganhos genéticos de 0,494, 1,229 e 1,500 kg ano-1, respectivamente. Em virtude da variação genética existente, verificou-se que a seleção do rebanho na região Norte do Brasil tem enfatizado principalmente a seleção para peso pós-desmama, ressaltando-se que a seleção para estes pesos pode gerar ao longo dos anos, aumento nos custos de produção, da idade ao abate e acabamento dos animais, havendo assim, necessidade de seleção mais criteriosa para estas características.Effective researches assessing the efficiency of cattle herds in Northern Brazil are incipient. This study aimed to evaluate the direct additive and maternal genetic trends of weights adjusted to 205 (P205, 365 (P365 and 550 (550 days of age in Nellore animals raised on pasture, born between 1997 and 2007, from Northern Brazil. Estimative of (covariance components used in the prediction of breeding values were obtained by derivative-free restricted maximum likelihood method (REML using the MTDFREML program. The genetic trends of direct and maternal genetic effects were obtained by the regression of the annual average of predicted breeding values

  19. ESAT-6 Targeting to DEC205+ Antigen Presenting Cells Induces Specific-T Cell Responses against ESAT-6 and Reduces Pulmonary Infection with Virulent Mycobacterium tuberculosis.

    Directory of Open Access Journals (Sweden)

    Aarón Silva-Sánchez

    Full Text Available Airways infection with Mycobacterium tuberculosis (Mtb is contained mostly by T cell responses, however, Mtb has developed evasion mechanisms which affect antigen presenting cell (APC maturation/recruitment delaying the onset of Ag-specific T cell responses. Hypothetically, bypassing the natural infection routes by delivering antigens directly to APCs may overcome the pathogen's naturally evolved evasion mechanisms, thus facilitating the induction of protective immune responses. We generated a murine monoclonal fusion antibody (α-DEC-ESAT to deliver Early Secretory Antigen Target (ESAT-6 directly to DEC205+ APCs and to assess its in vivo effects on protection associated responses (IFN-γ production, in vivo CTL killing, and pulmonary mycobacterial load. Treatment with α-DEC-ESAT alone induced ESAT-6-specific IFN-γ producing CD4+ T cells and prime-boost immunization prior to Mtb infection resulted in early influx (d14 post-infection and increased IFN-γ+ production by specific T cells in the lungs, compared to scarce IFN-γ production in control mice. In vivo CTL killing was quantified in relevant tissues upon transferring target cells loaded with mycobacterial antigens. During infection, α-DEC-ESAT-treated mice showed increased target cell killing in the lungs, where histology revealed cellular infiltrate and considerably reduced bacterial burden. Targeting the mycobacterial antigen ESAT-6 to DEC205+ APCs before infection expands specific T cell clones responsible for early T cell responses (IFN-γ production and CTL activity and substantially reduces lung bacterial burden. Delivering mycobacterial antigens directly to APCs provides a unique approach to study in vivo the role of APCs and specific T cell responses to assess their potential anti-mycobacterial functions.

  20. A putative autonomous 20.5 kb-CACTA transposon insertion in an F3'H allele identifies a new CACTA transposon subfamily in Glycine max

    Directory of Open Access Journals (Sweden)

    Vodkin Lila

    2008-12-01

    Full Text Available Abstract Background The molecular organization of very few genetically defined CACTA transposon systems have been characterized thoroughly as those of Spm/En in maize, Tam1 of Antirrhinum majus Candystripe1 (Cs1 from Sorghum bicolor and CAC1 from Arabidopsis thaliana, for example. To date, only defective deletion derivatives of CACTA elements have been described for soybean, an economically important plant species whose genome sequence will be completed in 2008. Results We identified a 20.5 kb insertion in a soybean flavonoid 3'-hydroxylase (F3'H gene representing the t* allele (stable gray trichome color whose origin traces to a single mutable chimeric plant displaying both tawny and gray trichomes. This 20.5 kb insertion has the molecular structure of a putative autonomous transposon of the CACTA family, designated Tgmt*. It encodes a large gene that was expressed in two sister isolines (T* and tm of the stable gray line (t* from which Tgmt* was isolated. RT-PCR derived cDNAs uncovered the structure of a large precursor mRNA as well as alternatively spliced transcripts reminiscent of the TNPA-mRNA generated by the En-1 element of maize but without sequence similarity to the maize TNPA. The larger mRNA encodes a transposase with a tnp2 and TNP1-transposase family domains. Because the two soybean lines expressing Tgmt* were derived from the same mutable chimeric plant that created the stable gray trichome t* allele line from which the element was isolated, Tgmt* has the potential to be an autonomous element that was rapidly inactivated in the stable gray trichome t* line. Comparison of Tgmt* to previously described Tgm elements demonstrated that two subtypes of CACTA transposon families exist in soybean based on divergence of their characteristic subterminal repeated motifs and their transposases. In addition, we report the sequence and annotation of a BAC clone containing the F3'H gene (T locus which was interrupted by the novel Tgmt* element

  1. 1-Pb/s (32 SDM/46 WDM/768 Gb/s) C-band Dense SDM Transmission over 205.6-km of Single-mode Heterogeneous Multi-core Fiber using 96-Gbaud PDM-16QAM Channels

    DEFF Research Database (Denmark)

    Kobayashi, Takayuki; Nakamura, M.; Hamaoka, F.

    2017-01-01

    We demonstrate the first 1-Pb/s unidirectional inline-amplified transmission over 205.6-km of single-mode 32-core fiber within C-band only. 96-Gbaud LDPC-coded PDM-16QAM channels with FEC redundancy of 12.75% realize high-aggregate spectral efficiency of 217.6 b/s/Hz......We demonstrate the first 1-Pb/s unidirectional inline-amplified transmission over 205.6-km of single-mode 32-core fiber within C-band only. 96-Gbaud LDPC-coded PDM-16QAM channels with FEC redundancy of 12.75% realize high-aggregate spectral efficiency of 217.6 b/s/Hz...

  2. NITRATE + NITRITE CONTENT (CONCENTRATION), PHOSPHATE, NITRITE, SILICATE and other profile and discrete sample data collected in the Gulf of Alaska on the ALPHA HELIX and WECOMA cruises HX203, HX205 and others as part of the NEP project from 1998-03-08 to 2004-07-05 (NODC Accession 0115260)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115260 includes profile, discrete sample and chemical data collected aboard the ALPHA HELIX and WECOMA during cruises HX203, HX205, HX208, HX211,...

  3. Diels-Alder Reactions of 12-Hydroxy-9(10®20-5aH-abeo-abieta-1(10,8(9,12(13-triene-11,14-dione

    Directory of Open Access Journals (Sweden)

    Shougang Hu

    2013-06-01

    Full Text Available 12-Hydroxy-9(10®20-5aH-abeo-abieta-1(10,8(9,12(13-triene-11,14-dione (quinone 2 served as the dienophile in numerous intermolecular Diels-Alder reactions. These cycloadditions were conducted either thermally (including microwave heating or with Lewis acid activation. While most dienes reacted with quinone 2 in good chemical yield, others were incompatible under the experimental conditions used.

  4. Thermochemistry of rare earth doped uranium oxides Ln x U 1-x O 2-0.5x+y (Ln = La, Y, Nd)

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Lei; Navrotsky, Alexandra

    2015-10-01

    Lanthanum, yttrium, and neodymium doped uranium dioxide samples in the fluorite structure have been synthesized, characterized in terms of metal ratio and oxygen content, and their enthalpies of formation measured by high temperature oxide melt solution calorimetry. For oxides doped with 10–50 mol % rare earth (Ln) cations, the formation enthalpies from constituent oxides (LnO1.5, UO2 and UO3 in a reaction not involving oxidation or reduction) become increasingly exothermic with increasing rare earth content, while showing no significant dependence on the varying uranium oxidation state. The oxidation enthalpy of LnxU1-xO2-0.5x+y is similar to that of UO2 to UO3 for all three rare earth doped systems. Though this may suggest that the oxidized uranium in these systems is energetically similar to that in the hexavalent state, thermochemical data alone can not constrain whether the uranium is present as U5+, U6+, or a mixture of oxidation states. The formation enthalpies from elements calculated from the calorimetric data are generally consistent with those from free energy measurements.

  5. Bioinformatics analysis for evaluation of the diagnostic potentialities of miR-19b, -125b and -205 as liquid biopsy markers of prostate cancer

    Science.gov (United States)

    Bryzgunova, O. E.; Lekchnov, E. A.; Zaripov, M. M.; Yurchenko, Yu. B.; Yarmoschuk, S. V.; Pashkovskaya, O. A.; Rykova, E. Yu.; Zheravin, A. A.; Laktionov, P. P.

    2017-09-01

    Presence of tumor-derived cell-free miRNA in biological fluids as well as simplicity and robustness of cell-free miRNA quantification makes them suitable markers for cancer diagnostics. Based on previously published data demonstrating diagnostic potentialities of miR-205 in blood and miR-19b as well as miR-125b in urine of prostate cancer patients, bioinformatics analysis was carried out to follow their involvement in prostate cancer development and select additional miRNA-markers for prostate cancer diagnostics. Studied miRNAs are involved in different signaling pathways and regulate a number of genes involved in cancer development. Five of their targets (CCND1, BRAF, CCNE1, CCNE2, RAF1), according to the STRING database, act as part of the same signaling pathway. RAF1 is regulated by miR-19b and miR-125b, and it was shown to be involved in prostate cancer development by DIANA and STRING databases. Thus, other microRNAs regulating RAF1 expression such as miR-16, -195, -497, and -7 (suggested by DIANA, TargetScan, MiRTarBase and miRDB databases) can potentially be regarded as prostate cancer markers.

  6. Depth profile of production yields of {sup nat}Pb(p, xn) {sup 206,205,204,203,202,201}Bi nuclear reactions

    Energy Technology Data Exchange (ETDEWEB)

    Mokhtari Oranj, Leila [Division of Advanced Nuclear Engineering, POSTECH, Pohang 37673 (Korea, Republic of); Jung, Nam-Suk; Kim, Dong-Hyun; Lee, Arim; Bae, Oryun [Pohang Accelerator Laboratory, POSTECH, Pohang 37673 (Korea, Republic of); Lee, Hee-Seock, E-mail: lee@postech.ac.kr [Pohang Accelerator Laboratory, POSTECH, Pohang 37673 (Korea, Republic of)

    2016-11-01

    Experimental and simulation studies on the depth profiles of production yields of {sup nat}Pb(p, xn) {sup 206,205,204,203,202,201}Bi nuclear reactions were carried out. Irradiation experiments were performed at the high-intensity proton linac facility (KOMAC) in Korea. The targets, irradiated by 100-MeV protons, were arranged in a stack consisting of natural Pb, Al, Au foils and Pb plates. The proton beam intensity was determined by activation analysis method using {sup 27}Al(p, 3p1n){sup 24}Na, {sup 197}Au(p, p1n){sup 196}Au, and {sup 197}Au(p, p3n){sup 194}Au monitor reactions and also by Gafchromic film dosimetry method. The yields of produced radio-nuclei in the {sup nat}Pb activation foils and monitor foils were measured by HPGe spectroscopy system. Monte Carlo simulations were performed by FLUKA, PHITS/DCHAIN-SP, and MCNPX/FISPACT codes and the calculated data were compared with the experimental results. A satisfactory agreement was observed between the present experimental data and the simulations.

  7. Depth profiles of production yields of natPb(p, xn206,205,204,203,202 Bi reactions using 100-MeV proton beam

    Directory of Open Access Journals (Sweden)

    Oranj Leila Mokhtari

    2017-01-01

    Full Text Available In this study, results of the experimental study on the depth profiles of production yields of 206,205,204,203,202Bi radio-nuclei in the natural Pb target irradiated by a 100-MeV proton beam are presented. Irradiation was performed at proton linac facility (KOMAC in Korea. The target, irradiated by 100-MeV protons, was arranged in a stack consisting of natural Pb, Al, Au foils and Pb plates. The proton beam intensity was determined by activation analysis method using 27Al(p, 3p1n24Na, 197Au(p, p1n196Au, and 197Au(p, p3n194Au monitor reactions and also using dosimetry method by a Gafchromic film. The production yields of produced Bi radio-nuclei in the natural Pb foils and monitor reactions were measured by gamma-ray spectroscopy. Monte Carlo simulations were performed by FLUKA, PHITS, and MCNPX codes and compared with the measurements in order to verify validity of physical models and nuclear data libraries in the Monte Carlo codes. A fairly good agreement was observed between the present experimental data and the simulations by FLUKA, PHITS, and MCNPX. However, physical models and the nuclear data relevant to the end of range of protons in the codes need to be improved.

  8. Clinical Outcomes of Volume-Modulated Arc Therapy in 205 Patients with Nasopharyngeal Carcinoma: An Analysis of Survival and Treatment Toxicities.

    Directory of Open Access Journals (Sweden)

    Rui Guo

    Full Text Available To investigate the clinical efficacy and treatment toxicity of volume-modulated arc therapy (VMAT for nasopharyngeal carcinoma (NPC.205 VMAT-treated NPC patients from our cancer center were prospectively entrolled. All patients received 68-70 Gy irradiation based on the planning target volume of the primary gross tumor volume. Acute and late toxicities were graded according to the Common Terminology Criteria for Adverse Events v3.0 and Radiation Therapy Oncology Group Late Radiation Morbidity Scoring Criteria.The median follow-up period was 37.3 months (range, 6.3-45.1 months. The 3-year estimated local failure-free survival, regional failure-free survival, locoregional failure-free survival, distant metastasis-free survival, disease-free survival and overall survival were 95.5%, 97.0%, 94.0%, 92.1%, 86.8% and 97.0%, respectively. Cox regression analysis showed primary gross tumor volume, N stage and EBV-DNA to be independent predictors of VMAT outcomes (P < 0.05. The most common acute and late side effects were grade 2-3 mucositis (78% and xerostomia (83%, 61%, 34%, and 9% at 3, 6, 12 and 24 months after VMAT, respectively.VMAT for the primary treatment of NPC achieved very high locoregional control with a favorable toxicity profile. The time-saving benefit of VMAT will enable more patients to receive precision radiotherapy.

  9. Frequency stabilization of a 2.05 μm laser using hollow-core fiber CO2 frequency reference cell

    Science.gov (United States)

    Meras, Patrick; Poberezhskiy, Ilya Y.; Chang, Daniel H.; Spiers, Gary D.

    2010-04-01

    We have designed and built a hollow-core fiber frequency reference cell, filled it with CO2, and used it to demonstrate frequency stabilization of a 2.05 μm Tm:Ho:YLF laser using frequency modulation (FM) spectroscopy technique. The frequency reference cell is housed in a compact and robust hermetic package that contains a several meter long hollow-core photonic crystal fiber optically coupled to index-guiding fibers with a fusion splice on one end and a mechanical splice on the other end. The package has connectorized fiber pigtails and a valve used to evacuate, refill it, or adjust the gas pressure. We have demonstrated laser frequency standard deviation decreasing from >450MHz (free-running) to laser wavelength is of particular interest for spectroscopic instruments due to the presence of many CO2 and H20 absorption lines in its vicinity. To our knowledge, this is the first reported demonstration of laser frequency stabilization at this wavelength using a hollow-core fiber reference cell. This approach enables all-fiber implementation of the optical portion of laser frequency stabilization system, thus making it dramatically more lightweight, compact, and robust than the traditional free-space version that utilizes glass or metal gas cells. It can also provide much longer interaction length of light with gas and does not require any alignment. The demonstrated frequency reference cell is particularly attractive for use in aircraft and space coherent lidar instruments for measuring atmospheric CO2 profile.

  10. Diallyl sulfide, diallyl disulfide, and diallyl trisulfide inhibit migration and invasion in human colon cancer colo 205 cells through the inhibition of matrix metalloproteinase-2, -7, and -9 expressions.

    Science.gov (United States)

    Lai, Kuang-Chi; Hsu, Shu-Chun; Kuo, Chao-Lin; Yang, Jai-Sing; Ma, Chia-Yu; Lu, Hsu-Feng; Tang, Nou-Ying; Hsia, Te-Chun; Ho, Heng-Chien; Chung, Jing-Gung

    2013-09-01

    Diallyl sulfide (DAS), diallyl disulfide (DADS), and diallyl trisulfide (DATS) are major organosulfur compounds exiting in garlic (Allium sativum). These compounds are reported to exhibit various pharmacological properties such as antibacteria, antiangiogenesis, anticancer, and anticoagulation, and they also induce cytotoxicity and induction of apoptosis in human cancer cells. Although these compounds show wide spectrum of biological activities, there are no reports to show that DAS, DADS, and DATS affected migration and invasion of human colon cancer cells, and their exact molecular mechanisms are not well investigated. Therefore, the purpose of this study was to determine whether DAS, DADS, and DATS affected the invasion and migration abilities of colo 205 human colon cancer cells. The results indicate that DAS, DADS, and DATS at 10 and 25 μM inhibited the migration and invasion of colo 205 cells in the order of DATS < DADS < DAS. DATS is the highest for inhibition of migration and invasion of colo 205 cells. DAS, DADS, and DATS induce downregulation expression of PI3K, Ras, MEKK3, MKK7, ERK1/2, JNK1/2, and p38 and then lead to the inhibition of MMP-2, -7, and -9. DAS, DADS, and DATS inhibited NF-κB and COX-2 for leading to the inhibition of cell proliferation. Taken together, these results demonstrated that application of DAS, DADS, and DATS might serve as potential antimetastatic drugs. Copyright © 2011 Wiley Periodicals, Inc., a Wiley company.

  11. ALMA [N ii] 205 μ m Imaging Spectroscopy of the Interacting Galaxy System BRI 1202-0725 at Redshift 4.7

    Energy Technology Data Exchange (ETDEWEB)

    Lu, Nanyao; Xu, C. Kevin; Zhu, Lei [National Astronomical Observatories, Chinese Academy of Sciences (CAS), Beijing 100012 (China); Zhao, Yinghe [Yunnan Observatories, Chinese Academy of Sciences, Kunming 650011 (China); Díaz-Santos, Tanio [Nucleo de Astronomia de la Facultad de Ingenieria, Universidad Diego Portales, Av. Ejercito Libertador 441, Santiago (Chile); Charmandaris, Vassilis [Department of Physics, University of Crete, GR-71003 Heraklion (Greece); Gao, Yu [Purple Mountain Observatory, CAS, Nanjing 210008 (China); Van der Werf, Paul P. [Leiden Observatory, Leiden University, P.O. Box 9513, 2300 RA Leiden (Netherlands); Privon, George C. [Departamento de Astronomía, Universidad de Concepción, Casilla 160-C, Concepción (Chile); Inami, Hanae [Centre de Recherche Astrophysique de Lyon (CRAL), Observatoire de Lyon, CNRS, UMR5574, F-69230, Saint-Genis-Laval (France); Rigopoulou, Dimitra [Department of Physics, University of Oxford, Keble Road, Oxford OX1 3RH (United Kingdom); Sanders, David B., E-mail: nanyao.lu@gmail.com [University of Hawaii, Institute for Astronomy, 2680 Woodlawn Drive, Honolulu, HI 96822 (United States)

    2017-06-20

    We present the results from Atacama Large Millimeter/submillimeter Array imaging in the [N ii] 205 μ m fine-structure line (hereafter [N ii]) and the underlying continuum of BRI 1202-0725, an interacting galaxy system at z = 4.7, consisting of a quasi-stellar object (QSO), a submillimeter galaxy (SMG), and two Ly α emitters, all within ∼25 kpc of the QSO. We detect the QSO and SMG in both [N ii] and continuum. At the ∼1″ (or 6.6 kpc) resolution, both the QSO and SMG are resolved in [N ii], with the de-convolved major axes of ∼9 and ∼14 kpc, respectively. In contrast, their continuum emissions are much more compact and unresolved even at an enhanced resolution of ∼0.″7. The ratio of the [N ii] flux to the existing CO(7−6) flux is used to constrain the dust temperature ( T {sub dust}) for a more accurate determination of the FIR luminosity L {sub FIR}. Our best estimated T {sub dust} equals 43 (±2) K for both galaxies (assuming an emissivity index β = 1.8). The resulting L {sub CO(7−6)}/ L {sub FIR} ratios are statistically consistent with that of local luminous infrared galaxies, confirming that L {sub CO(7−6)} traces the star formation (SF) rate (SFR) in these galaxies. We estimate that the ongoing SF of the QSO (SMG) has an SFR of 5.1 (6.9) × 10{sup 3} M {sub ⊙} yr{sup −1} (±30%) assuming Chabrier initial mass function, takes place within a diameter (at half maximum) of 1.3 (1.5) kpc, and will consume the existing 5 (5) × 10{sup 11} M {sub ⊙} of molecular gas in 10 (7) × 10{sup 7} years.

  12. Antifungal susceptibility of 205 Candida spp. isolated primarily during invasive Candidiasis and comparison of the Vitek 2 system with the CLSI broth microdilution and Etest methods.

    Science.gov (United States)

    Bourgeois, N; Dehandschoewercker, L; Bertout, S; Bousquet, P-J; Rispail, P; Lachaud, L

    2010-01-01

    Infections due to Candida spp. are frequent, particularly in immunocompromised and intensive care unit patients. Antifungal susceptibility tests are now required to optimize antifungal treatment given the emergence of acquired antifungal resistance in some Candida species. An antifungal susceptibility automated method, the Vitek 2 system (VK2), was evaluated. VK2 was compared to the CLSI broth microdilution reference method and the Etest procedure. For this purpose, 205 clinical isolates of Candida spp., including 11 different species, were tested for fluconazole, voriconazole, and amphotericin B susceptibility. For azoles, essential agreement ranged from 25% to 100%, depending on the method used and the Candida species tested. Categorical agreements for all of the species averaged 92.2% and ranged from 14.3 to 100%, depending on the 24-h or 48-h MIC reading by the Etest and CLSI methods and on the Candida species. Results obtained for Candida albicans showed excellent categorical and essential agreements with the two comparative methods. For Candida glabrata, the essential agreement was high with the CLSI method but low with the Etest method, and several very major errors in interpretation were observed between VK2 and the Etest method for both azoles. Low MICs of fluconazole were obtained for all of the Candida krusei isolates, but the VK2 expert software corrected all of the results obtained to resistant. Amphotericin B results showed MICs of CLSI), and 202 (Etest) isolates. The AST-YS01 Vitek 2 card system (bioMérieux) is a reliable and practical standardized automated antifungal susceptibility test. Nevertheless, more assays are needed to better evaluate C. glabrata fluconazole sensitivity.

  13. Antifungal Susceptibility of 205 Candida spp. Isolated Primarily during Invasive Candidiasis and Comparison of the Vitek 2 System with the CLSI Broth Microdilution and Etest Methods▿

    Science.gov (United States)

    Bourgeois, N.; Dehandschoewercker, L.; Bertout, S.; Bousquet, P.-J.; Rispail, P.; Lachaud, L.

    2010-01-01

    Infections due to Candida spp. are frequent, particularly in immunocompromised and intensive care unit patients. Antifungal susceptibility tests are now required to optimize antifungal treatment given the emergence of acquired antifungal resistance in some Candida species. An antifungal susceptibility automated method, the Vitek 2 system (VK2), was evaluated. VK2 was compared to the CLSI broth microdilution reference method and the Etest procedure. For this purpose, 205 clinical isolates of Candida spp., including 11 different species, were tested for fluconazole, voriconazole, and amphotericin B susceptibility. For azoles, essential agreement ranged from 25% to 100%, depending on the method used and the Candida species tested. Categorical agreements for all of the species averaged 92.2% and ranged from 14.3 to 100%, depending on the 24-h or 48-h MIC reading by the Etest and CLSI methods and on the Candida species. Results obtained for Candida albicans showed excellent categorical and essential agreements with the two comparative methods. For Candida glabrata, the essential agreement was high with the CLSI method but low with the Etest method, and several very major errors in interpretation were observed between VK2 and the Etest method for both azoles. Low MICs of fluconazole were obtained for all of the Candida krusei isolates, but the VK2 expert software corrected all of the results obtained to resistant. Amphotericin B results showed MICs of ≤1 mg/liter for 201 (VK2), 190 (CLSI), and 202 (Etest) isolates. The AST-YS01 Vitek 2 card system (bioMérieux) is a reliable and practical standardized automated antifungal susceptibility test. Nevertheless, more assays are needed to better evaluate C. glabrata fluconazole sensitivity. PMID:19889902

  14. 49 CFR 571.205 - Standard No. 205, Glazing materials.

    Science.gov (United States)

    2010-10-01

    ... providing shelter for persons. Glass-plastic glazing material means a laminate of one or more layers of glass and one or more layers of plastic in which a plastic surface of the glazing faces inward when the... coordinate system and point V1 shall be as specified in Annexes 18 and 19 of European Commission for Europe...

  15. Excitation functions of $^{nat}$Pb(d,x)$^{206,205,204,203,202}$Bi, $^{203cum,202m,201cum}$Pb and $^{202cum,201cum}$Tl reactions up to 50 MeV

    OpenAIRE

    Ditrói, F.; Tárkányi, F.; Takács, S.; Hermanne, A.; Ignatyuk, A. V.

    2014-01-01

    Cross-sections of deuteron induced nuclear reactions on lead were measured up to 50 MeV using the standard stacked foil irradiation technique and high resolution $\\gamma$-ray spectrometry. Experimental cross-sections and derived integral yields are presented for the $^{nat}$Pb(d,x)$^{206,205,204,203,202}$Bi, $^{203cum,202m,201cum}$Pb and $^{202cum,201cum}$Tl reactions. The experimental data were compared with the results from literature and with the data in the TENDL-2013 library (obtained wi...

  16. Rede de infarto com supradesnivelamento de ST: sistematização em 205 casos diminui eventos clínicos na rede pública

    Directory of Open Access Journals (Sweden)

    Ana Christina Vellozo Caluza

    2012-11-01

    Full Text Available FUNDAMENTO: A principal causa de óbitos na cidade de São Paulo (SP é por eventos cardíacos. Em hospitais periféricos de São Paulo estima-se a mortalidade hospitalar no infarto agudo entre 15% e 20%, pelas dificuldades existentes. OBJETIVO: Descrever a mortalidade intra-hospitalar do Infarto Agudo do Miocárdio com Supradesnivelamento de ST (IAMCSST de pacientes admitidos via ambulância ou hospitais periféricos, como resultado da organização de uma estruturada rede de treinamento. MÉTODOS: Equipes de quatro prontos-socorros (Ermelino Matarazzo, Campo Limpo, Tatuapé e Saboya e das ambulâncias avançadas do Serviço de Atendimento Móvel de Urgência (Samu foram treinadas para uso de tenecteplase (TNK ou para encaminhamento para angioplastia primária. Uma central de leitura de eletrocardiogramas foi usada quando necessário. Após uso de trombolítico, um hospital terciário recebia o paciente que era submetido a cinecoronariografia imediata (trombólise sem sucesso ou entre 6 e 24 h, caso estável. Variáveis quantitativas, qualitativas foram avaliadas em análise uni e multivariável. RESULTADOS: De janeiro 2010 a junho 2011, 205 pacientes consecutivos utilizaram a rede de atendimento, ocorrendo 87 infartos de parede anterior, 11 bloqueios de ramo esquerdo, 14 bloqueios atrioventricular total, e em 14 houve reanimação pós-parada cardiorrespiratória inicial. A mortalidade intra-hospitalar foi de 6,8% (14 casos, a maioria por choque cardiogênico, um por acidente vascular encefálico hemorrágico e um por sangramento. CONCLUSÃO: A organização em instituições públicas de uma rede de tratamento, envolvendo diagnóstico, reperfusão, transporte imediato e hospital de retaguarda resultou em melhora imediata dos resultados de IAMCSST.

  17. miR-29b, miR-205 and miR-221 enhance chemosensitivity to gemcitabine in HuH28 human cholangiocarcinoma cells.

    Directory of Open Access Journals (Sweden)

    Kinya Okamoto

    Full Text Available BACKGROUND AND AIMS: Cholangiocarcinoma (CCA is highly resistant to chemotherapy, including gemcitabine (Gem treatment. MicroRNAs (miRNAs are endogenous, non-coding, short RNAs that can regulate multiple genes expression. Some miRNAs play important roles in the chemosensitivity of tumors. Here, we examined the relationship between miRNA expression and the sensitivity of CCA cells to Gem. METHODS: Microarray analysis was used to determine the miRNA expression profiles of two CCA cell lines, HuH28 and HuCCT1. To determine the effect of candidate miRNAs on Gem sensitivity, expression of each candidate miRNA was modified via either transfection of a miRNA mimic or transfection of an anti-oligonucleotide. Ontology-based programs were used to identify potential target genes of candidate miRNAs that were confirmed to affect the Gem sensitivity of CCA cells. RESULTS: HuCCT1 cells were more sensitive to Gem than were HuH28 cells, and 18 miRNAs were differentially expressed whose ratios over ± 2log2 between HuH28 and HuCCT1. Among these 18 miRNAs, ectopic overexpression of each of three downregulated miRNAs in HuH28 (miR-29b, miR-205, miR-221 restored Gem sensitivity to HuH28. Suppression of one upregulated miRNA in HuH28, miR-125a-5p, inhibited HuH28 cell proliferation independently to Gem treatment. Selective siRNA-mediated downregulation of either of two software-predicted targets, PIK3R1 (target of miR-29b and miR-221 or MMP-2 (target of miR-29b, also conferred Gem sensitivity to HuH28. CONCLUSIONS: miRNA expression profiling was used to identify key miRNAs that regulate Gem sensitivity in CCA cells, and software that predicts miRNA targets was used to identify promising target genes for anti-tumor therapies.

  18. 197 - 205 _Saeed and Oladeji

    African Journals Online (AJOL)

    User

    ISSN 2006 – 6996. ASSESSMENT OF MANGANESE. VEGETABLE SAMPLES GROWN A. ZARIA, KADU .... MATERIALS AND METHODS. Study Area. Zaria city is in northern Nigeria on longitude 7˚42˙E ... Wastewater samples were collected using composite sampling in a polyethylene plastic container that were previously.

  19. Gasura 195-205.pmd

    African Journals Online (AJOL)

    Prof. Adipala Ekwamu

    Sweet potato virus disease (SPVD) is the most important disease of sweetpotato in the tropics. It causes yield losses of up to 98% and extinction of elite cultivars. Although there are no reports of immune cultivars, disease recovery phenomenon (a manifestation of some form of resistance) was reported in many vegetatively ...

  20. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to a combination of Lactobacillus gasseri PA 16/8, Bifidobacterium bifidum M 20/5 and Bifidobacterium longum SP 07/3 and maintenance of upper respiratory tract defence

    DEFF Research Database (Denmark)

    Tetens, Inge

    of Lactobacillus gasseri PA 16/8, Bifidobacterium bifidum M 20/5 and Bifidobacterium longum SP 07/3 and maintenance of upper respiratory tract defence against pathogens. The food constituent that is the subject of the claim, a combination of Lactobacillus gasseri PA 16/8, Bifidobacterium bifidum M 20....../5 and Bifidobacterium longum SP 07/3, is sufficiently characterised. The claimed effect, maintenance of upper respiratory tract defence against pathogens, is a beneficial physiological effect. The proposed target population is the general adult population. The Panel notes that the only human intervention study provided...... did not show an effect of consumption of a combination of Lactobacillus gasseri PA 16/8, Bifidobacterium bifidum M 20/5 and Bifidobacterium longum SP 07/3 on defence against pathogens in the upper respiratory tract. On the basis of the data presented, the Panel concludes that a cause and effect...

  1. Avaliação de Métodos de Padronização dos Pesos Corporais às Idades de 205, 365 e 550 Dias Evaluation of Body Weight Standardization Methods for 205, 365 and 550 Days of Age

    Directory of Open Access Journals (Sweden)

    Raimundo Nonato Braga Lôbo

    2002-07-01

    Full Text Available Foram comparados métodos de padronização dos pesos corporais às idades de 205, 365 e 550 dias. Utilizaram-se informações de animais da raça Nelore, criados na região Nordeste e controlados pela Associação Brasileira dos Criadores de Zebu (ABCZ. Compararam-se os métodos: MET1 - utilizado pela ABCZ; MET2 - curvas individuais de regressão linear para cada animal; MET3 - curvas individuais de regressão quadrática; MET4 - curvas individuais de Brody; MET5 - utilizado pelo Programa de Melhoramento Genético da Raça Nelore (PMGRN; e MET6 -- curvas individuais de Richards. Houve diferenças entre as médias dos pesos corporais calculadas pelos diferentes métodos. Foram verificadas altas correlações entre os métodos. As estimativas de variâncias e de parâmetros genéticos foram diferentes entre os métodos. Os métodos em execução no Brasil apresentam diferenças quanto à sua aproximação em representar a curva de crescimento dos animais. Apesar das altas correlações genéticas e de classificação dos animais por seus valores genéticos, as diferenças nesta classificação podem influenciar o processo de seleção e a resposta esperada à esta seleção.Methods of standardization of body weight for 205, 365 and 550 days of age were compared. It was used information of animals of the Nellore breed, raised in the Northeast Brazil, and controlled by the Brazilian Zebu Breeders Association (ABCZ. The following methods were compared: MET1 - used by ABCZ; MET2 - individual curves of linear regression for each animal; MET3 - individual curves of quadratic regression; MET4 - individual Brody curve; MET5 - used by the Program of Genetic Improvement of the Nellore breed (PMGRN; and MET6 -- individual Richards curves. There were differences among the means of the body weights calculated by the different methods. High correlations were verified among the methods. The variance and genetic parameters estimates were different among the

  2. Astatine-211 conjugated to an anti-CD20 monoclonal antibody eradicates disseminated B-cell lymphoma in a mouse model

    Energy Technology Data Exchange (ETDEWEB)

    Green, Damian J.; Shadman, Mazyar; Jones, Jon C.; Frayo, Shani; Kenoyer, Aimee L.; Hylarides, Mark; Hamlin, Donald K.; Wilbur, D. Scott; Balkan, Ethan R.; Lin, Yukang; Miller, Brian W.; Frost, Sophia; Gopal, Ajay K.; Orozco, Johnnie J.; Gooley, Ted; Laird, Kelley L.; Till, B. G.; Back, Tom; Sandmaier, B. M.; Pagel, John M.; Press, Oliver W.

    2015-03-26

    Alpha emitting radionuclides release a large amount of energy within a few cell diameters and may be particularly effective for radioimmunotherapy targeting minimal residual disease (MRD) conditions in which micrometastatic disease satellites are broadly distributed. To evaluate this hypothesis, 211At conjugated 1F5 mAb (anti-CD20) was studied in both bulky lymphoma tumor xenograft and MRD animal models. Superior treatment responses to 211At conjugated 1F5 mAb were evident in the MRD setting. Lymphoma xenograft tumor bearing animals treated with doses of up to 48µCi of anti-CD20 211At-decaborate [211At-B10-1F5] experienced modest responses (0% cures but 2-3-fold prolongation of survival compared to negative controls). In contrast, 70% of animals in the MRD lymphoma model demonstrated complete eradication of disease when treated with 211At-B10-1F5 at a radiation dose that was less than one-third (15 µCi) of the highest dose given to xenograft animals. Tumor progression among untreated control animals in both models was uniformly lethal. After 130 days, no significant renal or hepatic toxicity is observed in the cured animals receiving 15 µCi of 211At-B10-1F5. These findings suggest that in a MRD lymphoma model, where isolated cells and tumor microclusters prevail, α-emitters may be uniquely efficacious.

  3. Food sources of total omega 3 fatty acids (18:3 + 20:5 + 22:6), listed in descending order by percentages of their contribution to intake, based on data from the National Health and Nutrition Examination Survey 2005-2006

    Science.gov (United States)

    Food sources of total omega 3 fatty acids (18:3 + 20:5 + 22:6), listed in descending order by percentages of their contribution to intake, based on data from the National Health and Nutrition Examination Survey 2005-2006

  4. Millimeter- and submillimeter-wave surveys of Orion A emission lines in the ranges 200.7-202.3, 203.7-205.3, and 330-360 GHz

    Science.gov (United States)

    Jewell, P. R.; Hollis, J. M.; Lovas, F. J.; Snyder, L. E.

    1989-01-01

    A continuous spectral line survey of the Orion A position from 330.5 to 360.1 GHz was carried out. This survey covers nearly the entire 870 micron atmospheric window accessible from ground-based observations. Approximately 160 distinct spectral features composed of about 180 lines were detected, 29 of which could not be readily identified. In addition, Orion A from 200.7 to 202.3 GHz and from 203.7 to 205.3 GHz and 42 distinct new spectral lines were detected, including four that are unidentified at present. These data sets are the first thorough survey results in these spectral regions. The new interstellar lines in the survey bands are tabulated and displayed graphically. Moreover, the data are being made available to the Astronomical Data Center at the Goddard Space Flight Center for distribution by request to the astronomical community.

  5. Hb H disease resulting from the association of an αº-thalassemia allele [-(α20.5] with an unstable α-globin variant [Hb Icaria]: first report on the occurrence in Brazil

    Directory of Open Access Journals (Sweden)

    Elza M. Kimura

    2009-01-01

    Full Text Available Hb H Disease is caused by the loss or inactivation of three of the four functional a-globin genes. Patients present chronic hemolytic anemia and splenomegaly. In some cases, occasional blood transfusions are required. Deletions are the main cause of this type of thalassemia (α-thalassemia. We describe here an unusual case of Hb H disease caused by the combination of a common αº deletion [-(α20.5] with a rare point mutation (c.427T > A, thus resulting in an elongated and unstable α-globin variant, Hb Icaria, (X142K, with 31 additional amino-acid residues. Very high levels of Hb H and Hb Bart's were detected in the patient's red blood cells (14.7 and 19.0%, respectively. This is the first description of this infrequent association in the Brazilian population.

  6. Hydrogen and helium recycling from a JxB-force convected liquid metal Ga{sub 67}In{sub 20.5}Sn{sub 12.5} under steady state plasma bombardment

    Energy Technology Data Exchange (ETDEWEB)

    Hirooka, Yoshi, E-mail: hirooka.yoshihiko@nifs.ac.jp [National institute for Fusion Science, Oroshi, Toki, Gifu 509-5292 (Japan); Graduate University for Advanced Studies, Oroshi, Toki, Gifu 509-5292 (Japan); Bi, Hailin [Graduate University for Advanced Studies, Oroshi, Toki, Gifu 509-5292 (Japan); Shimada, Michiya [Japan Atomic Energy Agency, Rokkasho, Kamikita, Aomori 039-3212 (Japan); Ono, Masa [Princeton Plasma Physics Laboratory, Princeton, NJ 08543 (United States)

    2017-04-15

    A series of first-of-a-kind laboratory-scale experiments on the JxB-force convected liquid metal divertor concept have been carried out in the temperature range from room temperature to ∼200 °C, employing a eutectic alloy: Ga{sub 67}In{sub 20.5}Sn{sub 12.5}, the melting point of which is 10.5 °C. The electrical current conducted through the alloy is set at about 70A and the magnetic field is set at about 700 G. It has reproducibly been observed that hydrogen as well as helium particle recycling is noticeably reduced under steady state plasma bombardment when the liquid is convected by the JxB force.

  7. Circulating miR-19a and miR-205 in serum may predict the sensitivity of luminal A subtype of breast cancer patients to neoadjuvant chemotherapy with epirubicin plus paclitaxel.

    Directory of Open Access Journals (Sweden)

    Qian Li

    Full Text Available The luminal A subtype of breast cancer has a good prognosis and is sensitive to endocrine therapy but is less sensitive to chemotherapy. It is necessary to identify biomarkers to predict chemosensitivity and avoid over-treatment. We hypothesized that miRNAs in the serum might be associated with chemosensitivity.Sixty-eight breast cancer patients received neoadjuvant chemotherapy with epirubicin plus paclitaxel. The serum of the patients was collected before chemotherapy and stored at -80°C. The samples were classified into two groups in term of the chemosensitivity. We identified the differential expression patterns of miRNAs between the chemotherapy sensitive and resistant groups using microRNA profiling. Four miRNAs that were differentially expressed between the two groups were further validated in another 56 samples. We created a model fitting formula and a receiver operating characteristics (ROC curve using logistic regression analysis to evaluate the prediction potency.We identified 8 miRNAs differentially expressed between the two groups: 6 miRNAs were up-regulated, and 2 miRNAs were down-regulated in the resistant group compared with the sensitive group. The expression of miR-19a and miR-205 were determined to have significant differences between the two groups (P<0.05. A predictive model of these two miRNAs was created by the logistic regression analysis. The probability of this model was 89.71%. Based on the ROC curve, the specificity was 75.00%, and the sensitivity was 81.25%.The combination of miR-19a and miR-205 in the serum may predict the chemosensitivity of luminal A subtype of breast cancer to epirubicin plus paclitaxel neoadjuvant chemotherapy.

  8. Antibody-targeted NY-ESO-1 to mannose receptor or DEC-205 in vitro elicits dual human CD8+ and CD4+ T cell responses with broad antigen specificity.

    Science.gov (United States)

    Tsuji, Takemasa; Matsuzaki, Junko; Kelly, Marcus P; Ramakrishna, Venky; Vitale, Laura; He, Li-Zhen; Keler, Tibor; Odunsi, Kunle; Old, Lloyd J; Ritter, Gerd; Gnjatic, Sacha

    2011-01-15

    Immunization of cancer patients with vaccines containing full-length tumor Ags aims to elicit specific Abs and both CD4(+) and CD8(+) T cells. Vaccination with protein Ags, however, often elicits only CD4(+) T cell responses without inducing Ag-specific CD8(+) T cells, as exogenous protein is primarily presented to CD4(+) T cells. Recent data revealed that Ab-mediated targeting of protein Ags to cell surface receptors on dendritic cells could enhance the induction of both CD4(+) and CD8(+) T cells. We investigated in this study if these observations were applicable to NY-ESO-1, a cancer-testis Ag widely used in clinical cancer vaccine trials. We generated two novel targeting proteins consisting of the full-length NY-ESO-1 fused to the C terminus of two human mAbs against the human mannose receptor and DEC-205, both internalizing molecules expressed on APC. These targeting proteins were evaluated for their ability to activate NY-ESO-1-specific human CD4(+) and CD8(+) T cells in vitro. Both targeted NY-ESO-1 proteins rapidly bound to their respective targets on APC. Whereas nontargeted and Ab-targeted NY-ESO-1 proteins similarly activated CD4(+) T cells, cross-presentation to CD8(+) T cells was only efficiently induced by targeted NY-ESO-1. In addition, both mannose receptor and DEC-205 targeting elicited specific CD4(+) and CD8(+) T cells from PBLs of cancer patients. Receptor-specific delivery of NY-ESO-1 to APC appears to be a promising vaccination strategy to efficiently generate integrated and broad Ag-specific immune responses against NY-ESO-1 in cancer patients.

  9. Криминальный суицид в вопросах квалификации преступлений по ст. 205 и 205. 1 УК РФ

    OpenAIRE

    Гавло, Вениамин; Градусова, Марина

    2011-01-01

    Рассматривается использование террористическими организациями метода суицидальных террористических атак. Показано, что среди мотивированных причин суицида присутствуют криминальные или доведение до самоубийства. Предлагается применить термин «криминальный суицид» в практике определения составов преступлений по ст. 205 и 205.1 УК РФ, в отношении субъекта смертника, совершившего террористический акт, «криминальный суицидент»....

  10. Structure of neutron-rich nuclei around the N = 126 closed shell; the yrast structure of {sup 205}Au{sub 126} up to spin-parity I{sup {pi}} = (19/2{sup +})

    Energy Technology Data Exchange (ETDEWEB)

    Podolyak, Zs.; Steer, S.J.; Pietri, S.; Regan, P.H.; Brandau, C.; Catford, W.N.; Cullen, I.J.; Gelletly, W.; Jones, G.A.; Liu, Z.; Walker, P.M. [University of Surrey, Department of Physics, Guildford (United Kingdom); Gorska, M.; Gerl, J.; Wollersheim, H.J.; Grawe, H.; Becker, F.; Geissel, H.; Kelic, A.; Kojouharov, I.; Kurz, N.; Montes, F.; Prokopowicz, W.; Saito, T.; Schaffner, H.; Tashenov, S.; Werner-Malento, E. [GSI, Darmstadt (Germany); Rudolph, D.; Hoischen, R. [Lund University, Department of Physics, Lund (Sweden); Garnsworthy, A.B. [University of Surrey, Department of Physics, Guildford (United Kingdom); Yale University, WNSL, New Haven, CT (United States); Maier, K.H. [Institute of Nuclear Physics, Krakow (Poland); University of the West of Scotland, Dept. of Physics, Paisley (United Kingdom); Bednarczyk, P.; Grebosz, J. [GSI, Darmstadt (Germany); Institute of Nuclear Physics, Krakow (Poland); Caceres, L. [GSI, Darmstadt (Germany); Universidad Autonoma de Madrid, Dept. de Fisica Teorica, Madrid (Spain); Doornenbal, P. [GSI, Darmstadt (Germany); Universitaet zu Koeln, IKP, Koeln (Germany); Heinz, A. [Yale University, WNSL, New Haven, CT (United States); Kurtukian-Nieto, T. [Universidad de Santiago de Compostela, Santiago de Campostela (Spain); Benzoni, G.; Wieland, O. [Universita degli Studi di Milano (Italy); INFN, Milano (Italy); Pfuetzner, M. [Warsaw University, IEP, Warsaw (Poland); Jungclaus, A. [Universidad Autonoma de Madrid, Dept. de Fisica Teorica, Madrid (Spain); Balabanski, D.L. [Bulgarian Academy of Sciences, INRNE, Sofia (Bulgaria); Brown, B.A. [Univ. of Surrey, Dept. of Physics, Guildford (United Kingdom); Michigan State Univ., NSCL, East Lansing, MI (United States); Bruce, A.M.; Lalkovski, S. [Univ. of Brighton, School of Environment and Technology, Brighton (United Kingdom); Dombradi, Zs. [Institute for Nuclear Research, Debrecen (Hungary); Estevez, M.E. [Instituto de Fisica Corpuscular, Valencia (Spain)] [and others

    2009-12-15

    Heavy neutron-rich nuclei have been populated through the relativistic fragmentation of a {sup 208}{sub 82} Pb beam at E/A = 1 GeV on a 2.5 g/cm{sup 2} thick Be target. The synthesised nuclei were selected and identified in-flight using the fragment separator at GSI. Approximately 300 ns after production, the selected nuclei were implanted in an {proportional_to}8 mm thick perspex stopper, positioned at the centre of the RISING {gamma} -ray detector spectrometer array. A previously unreported isomer with a half-life T{sub 1/2} = 163(5) ns has been observed in the N=126 closed-shell nucleus {sup 205}{sub 79} Au. Through {gamma}-ray singles and {gamma}-{gamma} coincidence analysis a level scheme was established. The comparison with a shell model calculation tentatively identifies the spin-parity of the excited states, including the isomer itself, which is found to be I{sup {pi}} = (19/2{sup +}). (orig.)

  11. Structure of Bacteroides thetaiotaomicron BT2081 at 2.05 Å resolution: the first structural representative of a new protein family that may play a role in carbohydrate metabolism.

    Science.gov (United States)

    Yeh, Andrew P; Abdubek, Polat; Astakhova, Tamara; Axelrod, Herbert L; Bakolitsa, Constantina; Cai, Xiaohui; Carlton, Dennis; Chen, Connie; Chiu, Hsiu Ju; Chiu, Michelle; Clayton, Thomas; Das, Debanu; Deller, Marc C; Duan, Lian; Ellrott, Kyle; Farr, Carol L; Feuerhelm, Julie; Grant, Joanna C; Grzechnik, Anna; Han, Gye Won; Jaroszewski, Lukasz; Jin, Kevin K; Klock, Heath E; Knuth, Mark W; Kozbial, Piotr; Krishna, S Sri; Kumar, Abhinav; Lam, Winnie W; Marciano, David; McMullan, Daniel; Miller, Mitchell D; Morse, Andrew T; Nigoghossian, Edward; Nopakun, Amanda; Okach, Linda; Puckett, Christina; Reyes, Ron; Tien, Henry J; Trame, Christine B; van den Bedem, Henry; Weekes, Dana; Wooten, Tiffany; Xu, Qingping; Hodgson, Keith O; Wooley, John; Elsliger, Marc André; Deacon, Ashley M; Godzik, Adam; Lesley, Scott A; Wilson, Ian A

    2010-10-01

    BT2081 from Bacteroides thetaiotaomicron (GenBank accession code NP_810994.1) is a member of a novel protein family consisting of over 160 members, most of which are found in the different classes of Bacteroidetes. Genome-context analysis lends support to the involvement of this family in carbohydrate metabolism, which plays a key role in B. thetaiotaomicron as a predominant bacterial symbiont in the human distal gut microbiome. The crystal structure of BT2081 at 2.05 Å resolution represents the first structure from this new protein family. BT2081 consists of an N-terminal domain, which adopts a β-sandwich immunoglobulin-like fold, and a larger C-terminal domain with a β-sandwich jelly-roll fold. Structural analyses reveal that both domains are similar to those found in various carbohydrate-active enzymes. The C-terminal β-jelly-roll domain contains a potential carbohydrate-binding site that is highly conserved among BT2081 homologs and is situated in the same location as the carbohydrate-binding sites that are found in structurally similar glycoside hydrolases (GHs). However, in BT2081 this site is partially occluded by surrounding loops, which results in a deep solvent-accessible pocket rather than a shallower solvent-exposed cleft.

  12. High resolution spectroscopy in the second excited torsional state of $CH_{3}/OD$ and the atlas of the Fourier transform spectrum in the range 20-205 cm$^{-1}/$...

    CERN Document Server

    Mukhopadhyay, I

    1999-01-01

    In this work, the reduced eighth order Hamiltonian that has been used for the determination of the molecular parameters-using the high resolution Fourier transform far-infrared (FIR) assigned transitions in the ground and first $9 excited torsional states of CH/sub 3/OD-has been applied to assign and refine the model for the second excited torsional state. The data set consisted of 1220 FIR transitions with the rotational angular momentum J<21 and K<7 in the $9 second excited torsional state. It should be noted that the second excited torsional states of methanol are almost at the top of the torsional barrier. Thus it was interesting to see how the large amplitude motion is taken care of by $9 the eighth order Hamiltonian. We are in the process of a global fit including all the assigned transitions up to the second excited state. Additionally, in this paper we present the detailed Fourier transform spectral map of CH/sub $9 3/OD in the range 20-205 cm/sup -1/, as was done for the parent species. This sp...

  13. Altered levels of miR-21, miR-125b-2*, miR-138, miR-155, miR-184, and miR-205 in oral squamous cell carcinoma and association with clinicopathological characteristics.

    Science.gov (United States)

    Manikandan, Mayakannan; Deva Magendhra Rao, Arunagiri K; Rajkumar, Kottayasamy Seenivasagam; Rajaraman, Ramamurthy; Munirajan, Arasambattu K

    2015-11-01

    Previous studies have described the aberrantly expressed microRNAs (miRNAs) in oral squamous cell carcinoma (OSCC), and we reasoned that studying frequently deregulated candidate miRNAs in OSCC of Indian ethnicity could aid in better understanding of the genetic/environmental impact on the expression statuses of these miRNAs. Therefore, we evaluated the differential expression of six selected miRNAs namely hsa-miR-21, hsa-miR-125b2*, hsa-miR-138, hsa-miR-155, hsa-miR-184, and hsa-miR-205 in OSCC specimens of Indian ethnicity. Two-step Reverse transcriptase quantitative PCR using inventoried TaqMan single miRNA assays was employed to study the expression of the selected miRNAs in 42 OSCC tumors and eight adjacent normal specimens. The expression levels of the miRNAs were tested for any association with clinicopathological parameters. miR-21 was significantly elevated while miR-125b-2* was significantly downregulated in tumors compared to controls (P 184 were observed to be predominantly downregulated in the tumor samples. High levels of miR-155 were associated with the habit of chewing tobacco/betel quid. Our results corroborate the previous findings on the overexpression of mir-21 and downregulation of miR-138 in OSCC. As the expression of miR-184 is controversial in tongue/oral cancer, the downregulation may be specific to tumor anatomical localization. On the other hand, to the best of our knowledge, this is the first report to show the association of miR-155 with tobacco chewing and the downregulation of miR-125b-2* in OSCC. Computational predictions suggest that miR-125b-2* may have a role in alternative splicing. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  14. Long-Term Retrospective Clinical and Radiographic Follow-up of 205 Brånemark System Mk III TiUnite Implants Submitted to Either Immediate or Delayed Loading.

    Science.gov (United States)

    Imburgia, Mario; Del Fabbro, Massimo

    2015-10-01

    Studies are needed to evaluate long-term performance of immediately loaded implants with moderately rough surface. This retrospective study evaluated long-term survival and periimplant soft and hard tissue conditions in patients treated with TiUnite implants. Forty-one consecutive patients (mean age, 52.6 years) received 205 Brånemark System Mk III TiUnite implants (145 maxillary, 60 mandibular). The indication was single tooth (n = 7 implants), partial (n = 94), or full arches (n = 104). One hundred thirteen implants were immediately loaded. Cumulative survival rate (CSR) of implants was assessed. Long-term marginal bone remodeling, probing pocket depth (PPD), and periimplant mucosa conditions were assessed. Follow-up averaged 8.8 years (range, 6.6-10.6 years). Eight implants in 5 patients failed. CSR was 96.1% (implant basis) and 87.8% (patient basis) up to 10 years. At the longest follow-up, bone loss averaged 0.43 ± 1.15 mm (n = 173), PPD averaged 3.64 ± 0.74 mm, and periimplant mucosa was healthy in 74.6% of cases. Furthermore, 50.3% and 35.5% of implants scored negative for plaque and bleeding, respectively. No significant difference in CSR and hard and soft tissue conditions was found in the long term between immediately and delayed loaded implants. Implants with TiUnite surface demonstrated excellent long-term survival, marginal bone response, and soft tissue conditions, despite a nonoptimal level of oral hygiene.

  15. 76 FR 67628 - Airworthiness Directives; Bell Helicopter Textron, Inc. (Bell) Model 204B, 205A, 205A-1, 205B...

    Science.gov (United States)

    2011-11-02

    ... holes on yokes installed on Model 212 helicopters. That AD was intended to detect corrosion pitting and...-02, Bell has introduced a replacement stainless steel yoke, P/N 212-011-102. Bell issued Alert... configuration. The replacement stainless steel yokes have improved design characteristics addressing the...

  16. 75 FR 28751 - Airworthiness Directives; Bell Helicopter Textron (Bell) Model 205A, 205A-1, 205B, 212, 412...

    Science.gov (United States)

    2010-05-24

    ..., Martin R. Crane, ASW-170, Aviation Safety Engineer, Rotorcraft Directorate, Rotorcraft Certification.... Contact the Manager, Rotorcraft Certification Office: ATTN: DOT/FAA Southwest Region, Martin R. Crane, ASW..., Texas, on May 3, 2010. Mark R. Schilling, Acting Manager, Rotorcraft Directorate. BILLING CODE 4910-13-P ...

  17. Frontiers Of Change And Governance In Contractual Agreements: The Possible Role Of Exploitation - Uniting Reformed Church De Doorns V President Of The Republic Of South Africa 2013 5 Sa 205 (Wcc

    Directory of Open Access Journals (Sweden)

    Luanda Hawthorne

    2014-12-01

    Full Text Available In the South African common law of contract there appears to be support for the open norm of public policy as a general clause to ameliorate the effects of unfair contracts and terms which are contrary to public policy. The courts have on several occasions held that contracts or terms would be regarded as contrary to public policy had they come about where the parties were in an unequal bargaining relationship and this inequality was linked with another factor(s. In this case note it is argued that the element of unequal bargaining position may be contrary to public policy if it is linked with exploitation as the other factor. The element of exploitation was highlighted in the recent court decision of Uniting Reformed Church, De Doorns v President of the Republic of South Africa 2013 5 SA 205 (WCC. In this case the applicant church owned three properties on which were three schools under the control of the State. The church and the State had concluded 20-year notarial leases in respect of each of the properties. A term in the contract provided that after the expiration of the lease period the church would transfer the properties to the State free of charge. After the expiration of the leases the State demanded the transfer of the properties. The church disputed the claim, averring that the term was unenforceable because the parties had been in an unequal bargaining position and that the enforcement of the term constituted expropriation in contravention of the property clause of the Constitution. It is submitted that expropriation without compensation is not only contrary to section 25 of the Constitution but constitutes exploitation. It is suggested that where exploitation results from an unequal bargaining relationship it provides the "further factor" that, together with the inequality in bargaining power, is sufficient to establish that the term or contract is in conflict with public policy. This contributes to giving meaning to the term

  18. Temperature dependence of the photodissociation of CO2 from high vibrational levels: 205-230 nm imaging studies of CO(X1Σ+) and O(3P, 1D) products

    Science.gov (United States)

    Sutradhar, S.; Samanta, B. R.; Samanta, A. K.; Reisler, H.

    2017-07-01

    The 205-230 nm photodissociation of vibrationally excited CO2 at temperatures up to 1800 K was studied using Resonance Enhanced Multiphoton Ionization (REMPI) and time-sliced Velocity Map Imaging (VMI). CO2 molecules seeded in He were heated in an SiC tube attached to a pulsed valve and supersonically expanded to create a molecular beam of rotationally cooled but vibrationally hot CO2. Photodissociation was observed from vibrationally excited CO2 with internal energies up to about 20 000 cm-1, and CO(X1Σ+), O(3P), and O(1D) products were detected by REMPI. The large enhancement in the absorption cross section with increasing CO2 vibrational excitation made this investigation feasible. The internal energies of heated CO2 molecules that absorbed 230 nm radiation were estimated from the kinetic energy release (KER) distributions of CO(X1Σ+) products in v″ = 0. At 230 nm, CO2 needs to have at least 4000 cm-1 of rovibrational energy to absorb the UV radiation and produce CO(X1Σ+) + O(3P). CO2 internal energies in excess of 16 000 cm-1 were confirmed by observing O(1D) products. It is likely that initial absorption from levels with high bending excitation accesses both the A1B2 and B1A2 states, explaining the nearly isotropic angular distributions of the products. CO(X1Σ+) product internal energies were estimated from REMPI spectroscopy, and the KER distributions of the CO(X1Σ+), O(3P), and O(1D) products were obtained by VMI. The CO product internal energy distributions change with increasing CO2 temperature, suggesting that more than one dynamical pathway is involved when the internal energy of CO2 (and the corresponding available energy) increases. The KER distributions of O(1D) and O(3P) show broad internal energy distributions in the CO(X1Σ+) cofragment, extending up to the maximum allowed by energy but peaking at low KER values. Although not all the observations can be explained at this time, with the aid of available theoretical studies of CO2 VUV

  19. 40 CFR 205.2 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... Institute, Inc., 1430 Broadway, New York, New York 10018. (8) Sound Pressure Level means in decibels, 20... understood to be that of a root-mean-square pressure. The unit of any sound level is the decibel, having the unit symbol dB. (9) dB(A) means the standard abbreviation for A-weighted sound levels in decibels. (10...

  20. 24 CFR 964.205 - Eligibility.

    Science.gov (United States)

    2010-04-01

    ..., Board development, and leadership training; (ii) Determining the feasibility of resident management... contracts, and designing a long-range planning system. (3) Resident management business development. (i... Eligibility. (a) Resident councils/resident management corporations. Any eligible resident council/resident...

  1. 5 CFR 720.205 - Agency plans.

    Science.gov (United States)

    2010-01-01

    ..., and description of special targeted recruitment programs for such jobs and positions; (2) A similar... female candidates; (6) A description of efforts which will be undertaken by the agency to identify jobs... unit, nature of jobs and their wage or pay scale may be considered in focusing recruitment for various...

  2. 18 CFR 375.205 - Closed meetings.

    Science.gov (United States)

    2010-04-01

    ... a right to a fair trial or an impartial adjudication; (iii) Constitute an unwarranted invasion of... by a criminal law enforcement authority in the course of a criminal investigation, or by an agency... participation in a civil action or proceeding, an action in a foreign court or international tribunal, or an...

  3. 37 CFR 205.1 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... letters, deposits, recordations, registrations, publications, or other material submitted in connection... Copyright Office. Official business means the authorized business of the Copyright Office. Testimony means a...

  4. 7 CFR 1416.205 - Payment calculation.

    Science.gov (United States)

    2010-01-01

    ..., broilers, pullets; (3) Chickens, chicks; (4) Turkeys, toms, fryers, roasters; (5) Turkeys, poults; (6..., broilers, pullets; (22) Chickens, chicks; (23) Turkeys, toms, fryers, roasters; (24) Turkeys, poults; (25...

  5. 7 CFR 205.2 - Terms defined.

    Science.gov (United States)

    2010-01-01

    ..., leaves, or straw, or any synthetic material included on the National List for such use, such as newspaper or plastic that serves to suppress weed growth, moderate soil temperature, or conserve soil moisture...

  6. 12 CFR 205.2 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... Banking FEDERAL RESERVE SYSTEM BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM ELECTRONIC FUND TRANSFERS..., that may be used by the consumer to initiate electronic fund transfers. (2) An access device becomes an... another to use) the access device to transfer money between accounts or to obtain money, property, or...

  7. Publications | Page 205 | IDRC - International Development ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Within an exceptionally uncertain and dynamic vulnerability context this thesis advances proposals from the findings towards revising and developing the livelihoods inquiry. ''Resiliency perspective'' comprises people''s ability to mitigate the effects of adversity; their ability to thrive in a... CNDD-FDD in Burundi : the path from ...

  8. 7 CFR 205.681 - Appeals.

    Science.gov (United States)

    2010-01-01

    ... Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT... certifying agent may appeal the Program Manager's denial of accreditation or proposed suspension or...

  9. 7 CFR 205.680 - General.

    Science.gov (United States)

    2010-01-01

    ... Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT... National Organic Program's Program Manager may appeal such decision to the Administrator. (b) Persons...

  10. 7 CFR 205.660 - General.

    Science.gov (United States)

    2010-01-01

    ... Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT... Program's Program Manager, on behalf of the Secretary, may inspect and review certified production and...

  11. 9 CFR 205.206 - Farm products.

    Science.gov (United States)

    2010-01-01

    ... specify by name) Cotton Tobacco Flaxseed, peanuts, soybeans, sunflower seeds, other oil crops (system must... must specify by name) Apples, apricots, avocados, bananas, cherries, coffee, dates, figs, grapes... crops (system must specify by name) Grass seeds, legume seeds, other seed crops (system must specify by...

  12. 45 CFR 205.10 - Hearings.

    Science.gov (United States)

    2010-10-01

    ... a result of a judicial determination, or voluntarily placed in foster care by his legal guardian; (H... assessment other than that of the person or persons involved in making the original decision shall be... this requirement hearings shall meet the due process standards set forth in the U.S. Supreme Court...

  13. 15 CFR 970.205 - Vessel safety.

    Science.gov (United States)

    2010-01-01

    ... following: (1) That any foreign flag vessel whose flag state is party to the International Convention for Safety of Life at Sea, 1974 (SOLAS 74) possesses current valid SOLAS 74 certificates; (2) That any foreign flag vessel whose flag state is not party to SOLAS 74 but is party to the International Convention...

  14. 48 CFR 31.205-49 - Goodwill.

    Science.gov (United States)

    2010-10-01

    ... accounting for a business combination when the price paid by the acquiring company exceeds the sum of the identifiable individual assets acquired less liabilities assumed, based upon their fair values. The excess is commonly referred to as goodwill. Goodwill may arise from the acquisition of a company as a whole or a...

  15. Reference: 205 [Arabidopsis Phenome Database[Archive

    Lifescience Database Archive (English)

    Full Text Available of-function mutants for the AtXTH27 gene, xth27-1 and xth27-2. These exhibited short-shaped tracheary elements in tertiary... veins, and reduced the number of tertiary veins in the first leaf....ntation by introducing the wild-type XTH27 gene into xth27-1 mutant plants, the number of tertiary veins was

  16. 40 CFR 205.174 - Remedial orders.

    Science.gov (United States)

    2010-07-01

    ... microphone location point 1.2 ±0.1 m (3.9 ±0.3 ft) above the ground plane; and (B) Oriented in a plane... end point. (5) Throttle opening must be controlled to avoid excessive wheel slip or lift-off. (6) To... point 1.2 ±0.1 m (3.9 ±0.3 ft) above the ground plane; and (B) Oriented in a plane perpendicular to the...

  17. 10 CFR 205.351 - Reporting requirements.

    Science.gov (United States)

    2010-01-01

    ... maintaining the continuity of the bulk electric power system. (c) Vulnerabilities that could Impact System...) or terrorism directed at an electric power supply system, local or regional, in an attempt to either...

  18. 45 CFR 205.120 - Statewide operation.

    Science.gov (United States)

    2010-10-01

    ... assistance under title I, IV-A, X, XIV, or XVI (AABD) of the Social Security Act must provide that: (1) It...; and (ii) Regular planned examination and evaluation of operations in local offices by regularly assigned State staff, including regular visits by such staff; and through reports, controls, or other...

  19. Publications | Page 205 | IDRC - International Development ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Through books, articles, research publications, and studies, we aim to widen the impact of our investment and advance development research. ... (SSA), in the face of increased globalization and rapid trade liberalization during the past two decades has inspired considerable debate on the impact of globalization, in general, ...

  20. 24 CFR 266.205 - Ineligible projects.

    Science.gov (United States)

    2010-04-01

    ... kitchens and dining rooms with food service or mandatory services. (d) Nursing homes or intermediate care... URBAN DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES... services such as room service for food and beverages, maid service, furnishing and laundering of linens, or...

  1. 17 CFR 205.2 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... issuer with whom the attorney has an attorney-client relationship; or (ii) Is a non-appearing foreign... herself out as admitted, licensed, or otherwise qualified to practice law. (d) Breach of fiduciary duty refers to any breach of fiduciary or similar duty to the issuer recognized under an applicable Federal or...

  2. Publications | Page 205 | IDRC - International Development ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    IDRC works with developing-country researchers and institutions to build local capacity through funding, knowledge sharing, and training. Through books, articles, research publications, and studies, we aim to widen the impact of our investment and advance development research. We share the results of our funded ...

  3. 21 CFR 558.205 - Dichlorvos.

    Science.gov (United States)

    2010-04-01

    ... head until the medicated feed has been consumed. For boars, open or bred gilts, and sows, feed as sole... gastrointestinal tract. (ii) Limitations. For boars, open or bred gilts, and sows, feed as sole ration at the rate...) occurring in the gastrointestinal tract of the sow or gilt. (ii) Limitations. For pregnant swine; mix into a...

  4. 33 CFR 153.205 - Fines.

    Science.gov (United States)

    2010-07-01

    ... Federal Bldg., 500 Camp Street, New Orleans, LA 70130-3396 504-589-6901 9th 1240 E. 9th St., Cleveland, OH... and other portions 9th New Jersey: Upper portion 1st Lower portion 5th Puerto Rico 7th Virgin Islands...

  5. 48 CFR 1631.205-82 - Audits.

    Science.gov (United States)

    2010-10-01

    ... HEALTH BENEFITS ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND... accounting firms with which they contract for audits of FEHB accounts are registered with the Public Company Accounting Oversight Board (PCAOB). ...

  6. 24 CFR 51.205 - Mitigating measures.

    Science.gov (United States)

    2010-04-01

    .... Application of the standards for determining an Acceptable Separation Distance (ASD) for a HUD-assisted project from a potential hazard of an explosion or fire prone nature is predicated on level topography with no intervening object(s) between the hazard and the project. Application of the standards can be...

  7. 34 CFR 685.205 - Forbearance.

    Science.gov (United States)

    2010-07-01

    ... extension of time for making payments, or temporarily accepting smaller payments than previously scheduled... disabled, until the Secretary receives documentation of death or total and permanent disability; (6...

  8. 24 CFR 891.205 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... shopping, laundry, or getting to and from activities such as going to the doctor and shopping, but must be mobile. The mobility requirement does not exclude persons in wheelchairs or those requiring mobility... nonprofit entity, including a consumer cooperative: (1) No part of the net earnings of which inures to the...

  9. 27 CFR 26.205 - Certificate.

    Science.gov (United States)

    2010-04-01

    ... as to whether they were regauged when withdrawn from the insular bonded warehouse and, if regauged... §§ 26.260 and 26.302. (Approved by the Office of Management and Budget under control number 1512-0352...

  10. 40 CFR 1042.205 - Application requirements.

    Science.gov (United States)

    2010-07-01

    ... CONTROLS CONTROL OF EMISSIONS FROM NEW AND IN-USE MARINE COMPRESSION-IGNITION ENGINES AND VESSELS...: (1) Constant-speed engines. (2) Engines used with controllable-pitch propellers. (3) Recreational...

  11. 22 CFR 20.5 - Survivor benefits.

    Science.gov (United States)

    2010-04-01

    ... the principal, in the case of a principal who dies while in active service, including service on recall or reemployment while annuity is suspended or reduced; or, (4) 55 percent of the full annuity...

  12. 48 CFR 51.205 - Contract clause.

    Science.gov (United States)

    2010-10-01

    ... USE OF GOVERNMENT SOURCES BY CONTRACTORS Contractor Use of Interagency Fleet Management System (IFMS... Fleet Management System (IFMS) Vehicles and Related Services, in solicitations and contracts when a cost... interagency fleet management system (IFMS) vehicles and related services. FAR Index Federal Acquisition...

  13. 49 CFR 397.205 - Preemption application.

    Science.gov (United States)

    2010-10-01

    ..., including a State, political subdivision thereof, or Indian tribe directly affected by any highway routing designation of another State, political subdivision, or Indian tribe, may apply to the Administrator for a.... The Administrator shall publish notice of the application in the Federal Register. (b) Each...

  14. 13 CFR 400.205 - Application process.

    Science.gov (United States)

    2010-01-01

    ... appropriate environmental site assessments, and current personal and corporate financial statements of any... statements of guarantors shall be prepared by independent Certified Public Accountants; (8) Consolidated financial statements of the Borrower for the previous three years that have been audited by an independent...

  15. 13 CFR 500.205 - Application process.

    Science.gov (United States)

    2010-01-01

    ...(c)(3)(A)(ii) of the Act, is required to submit consolidated financial statements of the Borrower for... personal and corporate financial statements of any guarantors for the same periods as required for the... Standards Board of the Appraisal Foundation. Financial statements of guarantors shall be prepared by...

  16. 48 CFR 5.205 - Special situations.

    Science.gov (United States)

    2010-10-01

    ... before changing its basic purpose and mission, the sponsor must transmit at least three notices over a 90... procurement matters such as business fairs, long-range procurement estimates, prebid or preproposal... Circular A-76.) (f) Section 8(a) competitive acquisition. When a national buy requirement is being...

  17. {sup 203,205}Tl NMR Studies of Crystallographically Characterized Thallium Alkoxides. X-Ray Structures of [Tl(OCH{sub 2}CH{sub 3})]4 and [Tl(OAr)]{sub infinity} where OAr = OC{sub 6}H{sub 3}(Me){sub 2}-2,6 and OC{sub 6}H{sub 3}(Pr{sup i}){sub 2}-2,6

    Energy Technology Data Exchange (ETDEWEB)

    ZECHMANN,CECILIA A.; BOYLE,TIMOTHY J.; PEDROTTY,DAWN M.; ALAM,TODD M.; LANG,DAVID P.; SCOTT,BRIAN L.

    2000-07-25

    [Tl(OCH{sub 2}CH{sub 3})]{sub 4}, (1) was reacted with excess HOR to prepare a series of [Tl(OR)]{sub n} where OR= OCHMe{sub 2} (2, n = 4), OCMe{sub 3} (3, n = 4), OCH{sub 2}CMe{sub 3} (4, n = 4), OC{sub 6}H{sub 3}(Me){sub 2}-2,6 (5, n = {infinity}), and OC{sub 6}H{sub 3}(Pr{sup i}){sub 2}-2,6 (6, n = {infinity}). Single crystal X-ray diffraction was used to determine the structure of compounds ligated by more sterically demanding ligands. Compound 4 was found to adopt a cubane structure, while 5 and 6 formed linear polymeric structures. These compounds were additionally characterized by {sup 203,205}Tl solution and {sup 205}Tl solid state NMR. Compounds 1--4 were found to remain intact in solution while the polymeric species, 5 and 6, appeared to be fluxional. While variations in the solution and solid state structures for the tetrameric [Tl(OR)]{sub 4} and polymeric [Tl(OAr)]{sub {infinity}} may be influenced by the steric hindrance of their respective ligands, the covalency of the species is believed to be more an effect of the parent alcohol acidity.

  18. 76 FR 35334 - Airworthiness Directives; Bell Helicopter Textron, Inc. Model 205A, 205A-1, 205B, 212, 412, 412CF...

    Science.gov (United States)

    2011-06-17

    ... contains the AD, any comments, and other information on the Internet at http://www.regulations.gov , or in... 0.003 inch deep or less, either replace the unairworthy T/R blade with an airworthy T/R blade or... greater than 0.003 inch deep, replace the T/R blade with an airworthy T/R blade. (3) If any parent...

  19. Dicty_cDB: SLA205 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ynthesizing the nucleic acid. 52 3e-11 2 BD083024 |BD083024.1 Method of detecting difference between gene se...083025 |BD083025.1 Method of detecting difference between gene sequences. 52 5e-1...1652.1 Method for synthesizing the nucleic acid. 52 5e-11 2 BD083026 |BD083026.1 Method of detecting diffe...rence between gene sequences. 52 5e-11 2 BD181438 |BD181438.1 Method for synthesizi

  20. 205 Fulfillment of Social Responsibility as a Business Survival ...

    African Journals Online (AJOL)

    User

    2010-10-17

    Oct 17, 2010 ... Ethical behaviour can influence a firm's success in business. They stressed that it ranked the most important personal attributes that workers in most business organizations in the United States of America should posses. They, however, see social responsibility as a whole but observe that ethics concerns.