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Sample records for astatine 203

  1. Radiochemistry of astatine

    International Nuclear Information System (INIS)

    Ruth, T.J.; Dombsky, M.; D'Auria, J.M.; Ward, T.E.

    1988-01-01

    This monograph is a review of the literature through 1987 and covers the methods of producing the radioisotopes of astatine and the inorganic, nuclear, and organic chemistry of astatine. The discussion is limited to chemical and physical chemical properties of astatine. The monograph, after the introduction, is divided into chapters titled: production methods, nuclear spectroscopy, chemistry of astatine, separation and isolation (dry and wet), and selected procedures. 209 refs., 15 figs., 7 tabs

  2. Formation and decomposition of astatine molecules

    International Nuclear Information System (INIS)

    Takahashi, Naruto; Ishikuro, Mituhiro; Baba Hiroshi

    1989-01-01

    A method determining the boiling points of elementary astatine and astatine iodide has been developed (K. Otozai and N. Takahashi, Radio. Chim. Acta 31, (1982) 201). Further, it was concluded from the simple rule among the boiling point of elementary halogens and interhalogen compounds that elementary astatine might exist in diatomic molecules as the other halogens. In the present work the reaction mechanisms of elementary astatine with radioactive iodine and organic solvents were studied by means of radiogaschromatography in order to obtain further experimental evidences for diatomic astaine molecules. The following conclusions were obtained by the analysis of reaction kinetics. Two astatine atoms are lost from the elementary astatine fraction per each radioactive decay of astatine. The astatine radical or hot atom liberated by the decay of the complementary astatine atom immediately reacts with iodine or organic solvents. Thus formed astatine compounds decompose in turn due to the decay of astatine

  3. Organic astatine compounds, their preparation and properties

    Energy Technology Data Exchange (ETDEWEB)

    Vasaros, L; Berei, K

    1985-01-01

    Aromatic astatine compounds of possible medical application were prepared by high energy substitutions, by astatine-halogen, and by electrophil astatine-hydrogen substitutions at the Joint Institute of Nuclear Researches, Dubna. Physico-chemical properties of organic astatine compounds such as boiling point and evaporation heat, and the refraction and dissociation energy of carbon-astatine bonds were determined experimentally by gas chromatography. The results are compared with extrapolated data. (V.N.). 41 refs.; 7 figs.; 5 tables.

  4. Bibliography of astatine chemistry and biomedical applications

    International Nuclear Information System (INIS)

    Berei, K.; Vasaros, L.

    1992-02-01

    An overall bibliography is presented on astatine chemistry and on the biomedical applications of its 211 At isotope. The references were grouped in the following chapters: General reviews; Discovery, Natural Occurence; Nuclear Data; Preparation, Handling, Radiation Risk; Physico-chemical Properties; Astatine Compounds and Chemical Reactions; Biological Effects and Applications. Entries are sorted alphabetically by authors name in each chapter, and cross-references to other chapters are provided if appropriate. (R.P.)

  5. Recent advances in the organic chemistry of astatine

    International Nuclear Information System (INIS)

    Berei, K.; Vasaros, L.

    1994-03-01

    Investigation on the chemical behaviour of astatine in the last decade are surveyed. The survey covers the physical and chemical properties of astatine, synthesis and identification of organic astatine compounds, their physicochemical properties. A special chapter is devoted to biomedical applications, including inorganic 211 At species, 211 At-labelled proteins and drugs. An extensive bibliography of the related literature is given. (N.T.) 129 refs.; 12 figs.; 14 tabs

  6. Experimental and computational evidence of halogen bonds involving astatine

    Science.gov (United States)

    Guo, Ning; Maurice, Rémi; Teze, David; Graton, Jérôme; Champion, Julie; Montavon, Gilles; Galland, Nicolas

    2018-03-01

    The importance of halogen bonds—highly directional interactions between an electron-deficient σ-hole moiety in a halogenated compound and an acceptor such as a Lewis base—is being increasingly recognized in a wide variety of fields from biomedicinal chemistry to materials science. The heaviest halogens are known to form stronger halogen bonds, implying that if this trend continues down the periodic table, astatine should exhibit the highest halogen-bond donating ability. This may be mitigated, however, by the relativistic effects undergone by heavy elements, as illustrated by the metallic character of astatine. Here, the occurrence of halogen-bonding interactions involving astatine is experimentally evidenced. The complexation constants of astatine monoiodide with a series of organic ligands in cyclohexane solution were derived from distribution coefficient measurements and supported by relativistic quantum mechanical calculations. Taken together, the results show that astatine indeed behaves as a halogen-bond donor—a stronger one than iodine—owing to its much more electrophilic σ-hole.

  7. The reaction of astatine with aromatic diazonium compounds

    International Nuclear Information System (INIS)

    Visser, G.W.M.; Diemer, E.L.

    1982-01-01

    Astatine reacts prefrentially with that type of aromatic diazonium salt that decomposes via a radical reaction channel (homolytic breakage of the C-N bond). The dediazonation with p-aminobenzoic acid and p-toluidine as model compounds was investigated through estatin produced in the 209 Bi(α,2n) 211 At reaction. (author)

  8. Measurement of the first ionization potential of astatine by laser ionization spectroscopy

    Science.gov (United States)

    Rothe, S.; Andreyev, A. N.; Antalic, S.; Borschevsky, A.; Capponi, L.; Cocolios, T. E.; De Witte, H.; Eliav, E.; Fedorov, D. V.; Fedosseev, V. N.; Fink, D. A.; Fritzsche, S.; Ghys, L.; Huyse, M.; Imai, N.; Kaldor, U.; Kudryavtsev, Yuri; Köster, U.; Lane, J. F. W.; Lassen, J.; Liberati, V.; Lynch, K. M.; Marsh, B. A.; Nishio, K.; Pauwels, D.; Pershina, V.; Popescu, L.; Procter, T. J.; Radulov, D.; Raeder, S.; Rajabali, M. M.; Rapisarda, E.; Rossel, R. E.; Sandhu, K.; Seliverstov, M. D.; Sjödin, A. M.; Van den Bergh, P.; Van Duppen, P.; Venhart, M.; Wakabayashi, Y.; Wendt, K. D. A.

    2013-01-01

    The radioactive element astatine exists only in trace amounts in nature. Its properties can therefore only be explored by study of the minute quantities of artificially produced isotopes or by performing theoretical calculations. One of the most important properties influencing the chemical behaviour is the energy required to remove one electron from the valence shell, referred to as the ionization potential. Here we use laser spectroscopy to probe the optical spectrum of astatine near the ionization threshold. The observed series of Rydberg states enabled the first determination of the ionization potential of the astatine atom, 9.31751(8) eV. New ab initio calculations are performed to support the experimental result. The measured value serves as a benchmark for quantum chemistry calculations of the properties of astatine as well as for the theoretical prediction of the ionization potential of superheavy element 117, the heaviest homologue of astatine. PMID:23673620

  9. Automated astatination of biomolecules - a stepping stone towards multicenter clinical trials

    DEFF Research Database (Denmark)

    Aneheim, Emma; Albertsson, Per; Bäck, Tom

    2015-01-01

    To facilitate multicentre clinical studies on targeted alpha therapy, it is necessary to develop an automated, on-site procedure for conjugating rare, short-lived, alpha-emitting radionuclides to biomolecules. Astatine-211 is one of the few alpha-emitting nuclides with appropriate chemical...... vector, which can guide the radiation to the cancer cells. Consequently, an appropriate method is required for coupling the nuclide to the vector. To increase the availability of astatine-211 radiopharmaceuticals for targeted alpha therapy, their production should be automated. Here, we present a method...... challenging, alpha-emitting radionuclide. In this work, we describe the process platform, and we demonstrate the production of both astaine-211, for preclinical use, and astatine-211 labelled antibodies....

  10. Synthesis and Evaluation of Astatinated N-[2-(Maleimido)ethyl]-3-(trimethylstannyl)benzamide Immunoconjugates

    DEFF Research Database (Denmark)

    Aneheim, Emma; Gustafsson, Anna; Albertsson, Per

    2016-01-01

    Effective treatment of metastasis is a great challenge in the treatment of different types of cancers. Targeted alpha therapy utilizes the short tissue range (50-100 μm) of α particles, making the method suitable for treatment of disseminated occult cancers in the form of microtumors or even single...... to the antibody arbitrarily on lysine residues. By instead coupling astatine to disulfide bridges in the antibody structure, the immunoreactivity of the antibody conjugates could possibly be increased. Here, the disulfide-based conjugation was performed using a new coupling reagent, maleimidoethyl 3......-(trimethylstannyl)benzamide (MSB), and evaluated for chemical stability in vitro. The immunoconjugates were subsequently astatinated, resulting in both high radiochemical yield and high specific activity. The MSB-conjugate was shown to be stable with a long shelf life prior to the astatination. In a comparison...

  11. Some aspects of the organic, biological and inorganic chemistry of astatine

    International Nuclear Information System (INIS)

    Visser, G.W.M.

    1982-01-01

    Astatine has no stable isotopes and the radioactive isotopes with half-lives sufficiently long for chemical experiments ( 209 At, 210 At, 211 At) must be produced artificially with a cyclotron or with a high energy accelerator by spallation of Th. This thesis deals with the synthesis and chemistry of At-compounds and the determination of some of their properties. (C.F.)

  12. Direct astatination of a tumour-binding protein, human epidermal growth factor, using nido-carborane as a prosthetic group

    International Nuclear Information System (INIS)

    Sjoestroem, A.; Carlsson, J.; Lundqvist, H.; Koziorowski, J.

    2003-01-01

    A method for direct astatine labeling of proteins has been investigated. Binding sites for astatine were created by coupling of a nido-carborane derivative to a protein, the human epidermal growth factor (hEGF), using two different conjugation methods - by glutaraldehyde cross-linking or by introduction of sulfohydryl groups by Traut's reagent with subsequent linking of ANC-1 with m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester. The conjugates were astatinated using the Chloramine-T method in high yield. The best labeling was obtained by the glutaraldehyde conjugate with an average yield of 68 ± 9%. In vitro stability tests indicated that the glutaraldehyde conjugated label was as stable as hEGF labeled with astatobenzoate. (author)

  13. Estimation of the chemical form and the boiling point of elementary astatine by radiogas-chromatography

    International Nuclear Information System (INIS)

    Otozai, K.; Takahashi, N.

    1982-01-01

    After astatine (0) was mixed with 131 I 2 containing carrier I 2 , the sample was analyzed by means of radiogaschromatography and the peaks due to I 2 , AtI and At 2 were observed. Further, the boiling points were estimated from the retention volume in terms of the semi-empirical theory on gas chromatography. The boiling points of I 2 , AtI and At 2 were 457 +- 2,486 +- 2 and 503 +- 3K, respectively. The boiling point of At 2 obtained in the present work is far smaller than that expected by the extrapolation of lighter halogens. (orig.)

  14. Direct astatination of a tumour-binding protein, human epidermal growth factor, using nido-carborane as a prosthetic group

    Czech Academy of Sciences Publication Activity Database

    Sjostrom, A.; Tolmachev, V.; Lebeda, Ondřej; Koziorowski, J.; Carlsson, J.; Lundqvist, H.

    2003-01-01

    Roč. 256, č. 7 (2003), s. 191-197 ISSN 0236-5731 R&D Projects: GA AV ČR KSK4055109 Keywords : neutron-capture therapy * astatine-211 Subject RIV: CH - Nuclear ; Quantum Chemistry Impact factor: 0.472, year: 2003

  15. 211At-Rh(16-S4-diol) complex as a precursor for astatine radiopharmaceuticals

    International Nuclear Information System (INIS)

    Pruszynski, M.; Bilewicz, A.

    2006-01-01

    211 At is one of the most promising radionuclides in α-radioimmunotherapy (α-RIT). Unfortunately, biomolecules labeled by direct electrophilic astatination are unstable due to the rapid loss of 211 At under both in vitro and in vivo conditions. The present paper describes the results of our studies on attaching At - to the rhodium(III) complex with thioether ligand: 1,5,9,13-etrathiacyclohexadecane-3,11-diol (16-S4-diol). Rh 3+ was chosen as a moderately soft metal cation which should form very strong bonds with soft At - anions, but first of all because of the kinetic inertness of low spin rhodium(III) d 6 complexes. The 16-S4-diol ligand was selected due to formation of stable complexes with Rh 3+ . The experiments related to optimization of the reaction conditions were performed with the 131 I, basing on a chemical similarity of I - to At - . The experiments with 211 At were then carried out under the conditions found optimal for I - . The preliminary results are promising, and indicate a possibility for astatination of biomolecules by using the 211 At-Rh(16-S4-diol) complex

  16. Complexation study on no-carrier-added astatine with insulin: A candidate radiopharmaceutical

    Energy Technology Data Exchange (ETDEWEB)

    Lahiri, Susanta [Chemical Sciences Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700 064 (India)], E-mail: susanta.lahiri@saha.ac.in; Roy, Kamalika [Chemical Sciences Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700 064 (India); Sen, Souvik [Berhampur Sadar Hospital, Berhampur, Murshidabad 742 101 (India)

    2008-12-15

    No-carrier-added astatine radionuclides produced in the {sup 7}Li-irradiated lead matrix were separated from bulk lead nitrate target by complexing At with insulin, followed by dialysis. The method offers simultaneous separation of At from lead as well as its complexation with insulin. The At-insulin complex might be a potential radiopharmaceutical in the treatment of hepatocellular carcinoma. The stability of At-insulin complex was checked by dialysis against deionized water and Ringer lactate (RL) solution. It has been found that the half-life of At-insulin complex is about {approx}12 h, when dialyzed against deionized water and is only 6 h, when dialyzed against RL solution having the same composition as blood serum. The 6 h half-life of this Insulin-At complex is perfect for killing cancer cells from external cell surfaces as the half-life of internalization of insulin molecule inside the cell is 7-12 h.

  17. Laser photodetachment of radioactive ions: towards the determination of the electronegativity of astatine

    CERN Multimedia

    Rothe, Sebastian; Welander, Jakob Emanuel; Chrysalidis, Katerina; Day Goodacre, Thomas; Fedosseev, Valentine; Fiotakis, Spyridon; Forstner, Oliver; Heinke, Reinhard Matthias; Johnston, Karl; Kron, Tobias; Koester, Ulli; Liu, Yuan; Marsh, Bruce; Ringvall Moberg, Annie; Rossel, Ralf Erik; Seiffert, Christoph; Studer, Dominik; Wendt, Klaus; Hanstorp, Dag

    2017-01-01

    Negatively charged ions are mainly stabilized through the electron correlation effect. A measure of the stability of a negative ion is the electron affinity, which the energy gain by attaching an electron to a neutral atom. This fundamental quantity is, due to the almost general lack of bound excited states, the only atomic property that can be determined with high accuracy for negative ions. We will present the results of the first laser photodetachment studies of radioactive negative ions at CERN-ISOLDE. The photodetachment threshold for the radiogenic iodine isotope 128I was measured successfully, demonstrating the performance of the upgraded GANDALPH experimental beam line. The first detection of photo-detached astatine atoms marks a milestone towards the determination of the EA of this radioactive element.

  18. Thermogravimetric determination of the enthalpy of astatine and radon adsorption on palladium surfaces

    International Nuclear Information System (INIS)

    Eichler, B.; Son Chun, K.

    1985-01-01

    In order to investigate the adsorption of astatine and radon on a palladium surface some on- and off-line thermochromatographic experiments were carried out with 210 At and 220 Rn tracers. The partial molar adsorption enthalpy for zero covering was found to be ΔH/sub a//sup 0, loc./(At) = -(15S +- 10) kJ mole -1 and ΔH/sub a//sup 0, mob./(Rn) = -(37 +- 4) kJ mole -1 . The results are compared with theoretical and experimental values for other elements of the sixth period. The adsorption behaviour of At is in conformity with that of the p-metals on a palladium surface. (author)

  19. Determination of the electron affinity of astatine and polonium by laser photodetachment

    CERN Multimedia

    We propose to conduct the first electron affinity (EA) measurements of the two elements astatine (At) and polonium (Po). Collinear photo-detachment spectroscopy will allow us to measure these quantities with an uncertainty limited only by the spectral line width of the laser. We plan to use negative ion beams of the two radioactive elements At and Po, which are only accessible on-line and at ISOLDE. The feasibility of our proposed method and the functionality of the experimental setup have been demonstrated at ISOLDE in off-line tests by the clear observation of the photo-detachment threshold for stable iodine. This proposal is based on our Letter of Intent I-148.

  20. Complexation study on no-carrier-added astatine with insulin: A candidate radiopharmaceutical

    International Nuclear Information System (INIS)

    Lahiri, Susanta; Roy, Kamalika; Sen, Souvik

    2008-01-01

    No-carrier-added astatine radionuclides produced in the 7 Li-irradiated lead matrix were separated from bulk lead nitrate target by complexing At with insulin, followed by dialysis. The method offers simultaneous separation of At from lead as well as its complexation with insulin. The At-insulin complex might be a potential radiopharmaceutical in the treatment of hepatocellular carcinoma. The stability of At-insulin complex was checked by dialysis against deionized water and Ringer lactate (RL) solution. It has been found that the half-life of At-insulin complex is about ∼12 h, when dialyzed against deionized water and is only 6 h, when dialyzed against RL solution having the same composition as blood serum. The 6 h half-life of this Insulin-At complex is perfect for killing cancer cells from external cell surfaces as the half-life of internalization of insulin molecule inside the cell is 7-12 h

  1. Astatine-211 Radiochemistry: The Development Of Methodologies For High Activity Level Radiosynthesis

    International Nuclear Information System (INIS)

    Zalutsky, Michael R.

    2012-01-01

    Targeted radionuclide therapy is emerging as a viable approach for cancer treatment because of its potential for delivering curative doses of radiation to malignant cell populations while sparing normal tissues. Alpha particles such as those emitted by 211At are particularly attractive for this purpose because of their short path length in tissue and high energy, making them highly effective in killing cancer cells. The current impact of targeted radiotherapy in the clinical domain remains limited despite the fact that in many cases, potentially useful molecular targets and labeled compounds have already been identified. Unfortunately, putting these concepts into practice has been impeded by limitations in radiochemistry methodologies. A critical problem is that the synthesis of therapeutic radiopharmaceuticals provides additional challenges in comparison to diagnostic reagents because of the need to perform radio-synthesis at high levels of radioactivity. This is particularly important for α-particle emitters such as 211At because they deposit large amounts of energy in a highly focal manner. The overall objective of this project is to develop convenient and reproducible radiochemical methodologies for the radiohalogenation of molecules with the α-particle emitter 211At at the radioactivity levels needed for clinical studies. Our goal is to address two problems in astatine radiochemistry: First, a well known characteristic of 211At chemistry is that yields for electrophilic astatination reactions decline as the time interval after radionuclide isolation from the cyclotron target increases. This is a critical problem that must be addressed if cyclotrons are to be able to efficiently supply 211At to remote users. And second, when the preparation of high levels of 211At-labeled compounds is attempted, the radiochemical yields can be considerably lower than those encountered at tracer dose. For these reasons, clinical evaluation of promising 211At-labeled targeted

  2. ASTATINE-211 RADIOCHEMISTRY: THE DEVELOPMENT OF METHODOLOGIES FOR HIGH ACTIVITY LEVEL RADIOSYNTHESIS

    Energy Technology Data Exchange (ETDEWEB)

    MICHAEL R. ZALUTSKY

    2012-08-08

    Targeted radionuclide therapy is emerging as a viable approach for cancer treatment because of its potential for delivering curative doses of radiation to malignant cell populations while sparing normal tissues. Alpha particles such as those emitted by 211At are particularly attractive for this purpose because of their short path length in tissue and high energy, making them highly effective in killing cancer cells. The current impact of targeted radiotherapy in the clinical domain remains limited despite the fact that in many cases, potentially useful molecular targets and labeled compounds have already been identified. Unfortunately, putting these concepts into practice has been impeded by limitations in radiochemistry methodologies. A critical problem is that the synthesis of therapeutic radiopharmaceuticals provides additional challenges in comparison to diagnostic reagents because of the need to perform radio-synthesis at high levels of radioactivity. This is particularly important for {alpha}-particle emitters such as 211At because they deposit large amounts of energy in a highly focal manner. The overall objective of this project is to develop convenient and reproducible radiochemical methodologies for the radiohalogenation of molecules with the {alpha}-particle emitter 211At at the radioactivity levels needed for clinical studies. Our goal is to address two problems in astatine radiochemistry: First, a well known characteristic of 211At chemistry is that yields for electrophilic astatination reactions decline as the time interval after radionuclide isolation from the cyclotron target increases. This is a critical problem that must be addressed if cyclotrons are to be able to efficiently supply 211At to remote users. And second, when the preparation of high levels of 211At-labeled compounds is attempted, the radiochemical yields can be considerably lower than those encountered at tracer dose. For these reasons, clinical evaluation of promising 211At

  3. Part I: $\\beta$-delayed fission, laser spectroscopy and shape-coexistence studies with astatine beams; Part II: Delineating the island of deformation in the light gold isotopes by means of laser spectroscopy

    CERN Document Server

    Andreyev, Andrei

    2013-01-01

    Part I: $\\beta$-delayed fission, laser spectroscopy and shape-coexistence studies with astatine beams; Part II: Delineating the island of deformation in the light gold isotopes by means of laser spectroscopy

  4. In vitro evaluation of the astatinated chimeric monoclonal antibody U36, a potential candidate for treatment of head and neck squamous cell carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Nestor, M.; Anniko, M. [Uppsala University, Unit of Otolaryngology and Head and Neck Surgery, Department of Surgical Sciences, Uppsala (Sweden); Persson, M. [Uppsala University, Unit of Urology, Department of Surgical Sciences, Uppsala (Sweden); Uppsala University, Unit of Biomedical Radiation Science, Department of Oncology, Radiology and Clinical Immunology, Uppsala (Sweden); Dongen, G.A.M.S. van [Vrije Universiteit Medical Center, Department of Otolaryngology/Head and Neck Surgery, Amsterdam (Netherlands); Jensen, H.J. [Righshospitalet, PET and Cyclotron Unit, Department of Clinical Physiology and Nuclear Medicine, Copenhagen (Denmark); Lundqvist, H.; Tolmachev, V. [Uppsala University, Unit of Biomedical Radiation Science, Department of Oncology, Radiology and Clinical Immunology, Uppsala (Sweden)

    2005-11-01

    The purpose of this study was to analyse the properties of the astatinated chimeric MAb (cMAb) U36 as a conjugate to selectively target and eradicate head and neck squamous cell carcinoma (HNSCC). cMAb U36 was labelled with {sup 211}At via the linker N-succinimidyl 4-(trimethylstannyl)benzoate (SPMB). The quality of the conjugate was extensively evaluated for binding and internalisation capacity, and compared with {sup 125}I-SPMB-cMAb U36. The cellular toxicity of the astatinated conjugate was assessed in two types of in vitro growth assay and compared with {sup 131}I-labelled cMAb U36 (directly labelled). Comparisons between {sup 211}At-cMAb U36 and {sup 125}I-cMAb U36 demonstrated an optimal functional capacity of the labelled products. Immunoreactivity and affinity assays showed high immunoreactive fractions (>93%), and an affinity in good agreement between the astatinated and iodinated antibodies. For both conjugates, specific binding to HNSCC cells could be demonstrated, as well as some internalisation. Retention of the astatinated conjugate was just slightly lower than for the iodinated conjugate and still reasonable for therapeutic use (31{+-}2% vs 42.6{+-}1.0% at 22 h), demonstrating no adverse effects from astatination of the antibody. Studies on cellular toxicity demonstrated a dose-dependent and antigen-specific cellular toxicity for {sup 211}At-cMAb U36, with about 10% cell survival at 50 decays per cell. The {sup 131}I-labelled conjugate was not as efficient, with a surviving cell fraction of about 50% at 55 decays per cell. (orig.)

  5. In vitro evaluation of the astatinated chimeric monoclonal antibody U36, a potential candidate for treatment of head and neck squamous cell carcinoma

    International Nuclear Information System (INIS)

    Nestor, M.; Anniko, M.; Persson, M.; Dongen, G.A.M.S. van; Jensen, H.J.; Lundqvist, H.; Tolmachev, V.

    2005-01-01

    The purpose of this study was to analyse the properties of the astatinated chimeric MAb (cMAb) U36 as a conjugate to selectively target and eradicate head and neck squamous cell carcinoma (HNSCC). cMAb U36 was labelled with 211 At via the linker N-succinimidyl 4-(trimethylstannyl)benzoate (SPMB). The quality of the conjugate was extensively evaluated for binding and internalisation capacity, and compared with 125 I-SPMB-cMAb U36. The cellular toxicity of the astatinated conjugate was assessed in two types of in vitro growth assay and compared with 131 I-labelled cMAb U36 (directly labelled). Comparisons between 211 At-cMAb U36 and 125 I-cMAb U36 demonstrated an optimal functional capacity of the labelled products. Immunoreactivity and affinity assays showed high immunoreactive fractions (>93%), and an affinity in good agreement between the astatinated and iodinated antibodies. For both conjugates, specific binding to HNSCC cells could be demonstrated, as well as some internalisation. Retention of the astatinated conjugate was just slightly lower than for the iodinated conjugate and still reasonable for therapeutic use (31±2% vs 42.6±1.0% at 22 h), demonstrating no adverse effects from astatination of the antibody. Studies on cellular toxicity demonstrated a dose-dependent and antigen-specific cellular toxicity for 211 At-cMAb U36, with about 10% cell survival at 50 decays per cell. The 131 I-labelled conjugate was not as efficient, with a surviving cell fraction of about 50% at 55 decays per cell. (orig.)

  6. High-efficiency astatination of antibodies using N-iodosuccinimide as the oxidising agent in labelling of N-succinimidyl 3-(trimethylstannyl)benzoate

    International Nuclear Information System (INIS)

    Lindegren, S.; Andersson, H.; Baeck, T.; Jacobsson, L.; Karlsson, B.; Skarnemark, G.

    2001-01-01

    Monoclonal antibodies C215, reactive with colorectal carcinomas, and MOv18, reactive with most of the ovarian carcinomas, were radiohalogenated with [ 211 At]astatine. The radiohalogen was conjugate coupled to antibodies via the intermediate labelling reagent N-succinimidyl-3-(trimethylstannyl)benzoate (m-MeATE) in a two-step, single-pot reaction. Optimisation of the labelling of the reagent was achieved using N-iodosuccinimide, NIS, as the oxidising agent. The yields ranged from 69-95% in the labelling of 0.1-1.0 nmole of the m-MeATE precursor. Subsequent conjugation to antibodies resulted in yields of 58±7%. In vitro binding to tumour cells showed that the immunoreactivity of both antibodies was retained after astatine labelling

  7. An all-solid state laser system for the laser ion source RILIS and in-source laser spectroscopy of astatine at ISOLDE, CERN

    CERN Document Server

    Rothe, Sebastian; Nörtershäuser, W

    This doctoral thesis describes the extension of the resonance ionization laser ion source RILIS at ISOLDE, CERN, by the addition of an all-solid state tuneable titanium: sapphire (Ti:Sa) laser system to complement the well-established system of dye lasers. Synchronous operation of the so called Dual RILIS system of Ti:Sa and dye lasers was investigated and the potential for increased ion beam intensity, reliability, and reduced setup time has been demonstrated. In-source resonance ionization spectroscopy was performed at ISOLDE, CERN, and at ISAC, TRIUMF, radioactive ion beam facilities to develop an efficient and selective three-colour ionization scheme for the purely radioactive element astatine. A LabVIEW based monitoring, control and measurement system was conceived which enabled, in conjunction with Dual RILIS operation, the spectroscopy of high lying Rydberg states, from which the ionization potential of the astatine atom was determined for the first time experimentally.

  8. An all-solid state laser system for the laser ion sources RILIS and in-source laser spectroscopy of astatine at ISOLDE/CERN

    International Nuclear Information System (INIS)

    Rothe, Sebastian

    2012-01-01

    This doctoral thesis describes the extension of the resonance ionization laser ion source RILIS at CERN/ISOLDE by the addition of an all-solid state tunable titanium:sapphire (Ti:Sa) laser system to complement the well-established system of dye lasers. Synchronous operation of the so called Dual RILIS system of Ti:Sa and dye lasers was investigated and the potential for increased ion beam intensity, reliability, and reduced setup time has been demonstrated. In-source resonance ionization spectroscopy was performed at ISOLDE/CERN and at ISAC/TRIUMF radioactive ion beam facilities to develop an efficient and selective three-colour ionization scheme for the purely radioactive element astatine. A LabVIEW based monitoring, control and measurement system was conceived which enabled, in conjunction with Dual RILIS operation, the spectroscopy of high lying Rydberg states, from which the ionization potential of the astatine atom was determined for the first time experimentally.

  9. An all-solid state laser system for the laser ion sources RILIS and in-source laser spectroscopy of astatine at ISOLDE/CERN

    Energy Technology Data Exchange (ETDEWEB)

    Rothe, Sebastian

    2012-09-24

    This doctoral thesis describes the extension of the resonance ionization laser ion source RILIS at CERN/ISOLDE by the addition of an all-solid state tunable titanium:sapphire (Ti:Sa) laser system to complement the well-established system of dye lasers. Synchronous operation of the so called Dual RILIS system of Ti:Sa and dye lasers was investigated and the potential for increased ion beam intensity, reliability, and reduced setup time has been demonstrated. In-source resonance ionization spectroscopy was performed at ISOLDE/CERN and at ISAC/TRIUMF radioactive ion beam facilities to develop an efficient and selective three-colour ionization scheme for the purely radioactive element astatine. A LabVIEW based monitoring, control and measurement system was conceived which enabled, in conjunction with Dual RILIS operation, the spectroscopy of high lying Rydberg states, from which the ionization potential of the astatine atom was determined for the first time experimentally.

  10. Radiobiological Effects of Alpha-Particles from Astatine-211: From DNA Damage to Cell Death

    Energy Technology Data Exchange (ETDEWEB)

    Claesson, Kristina

    2011-05-15

    In recent years, the use of high linear energy transfer (LET) radiation for radiotherapeutic applications has gained increased interest. Astatine-211 (211At) is an alpha-particle emitting radionuclide, promising for targeted radioimmunotherapy of isolated tumor cells and microscopic clusters. To improve development of safe radiotherapy using 211At it is important to increase our knowledge of the radiobiological effects in cells. During radiotherapy, both tumors and adjacent normal tissue will be irradiated and therefore, it is of importance to understand differences in the radio response between proliferating and resting cells. The aim of this thesis was to investigate effects in fibroblasts with different proliferation status after irradiation with alpha-particles from 211At or X-rays, from inflicted DNA damage, to cellular responses and biological consequences. Throughout this work, irradiation was performed with alpha-particles from 211A or X-rays. The induction and repair of double-strand breaks (DSBs) in human normal fibroblasts were investigated using pulsed-field gel electrophoresis and fragment analysis. The relative biological effectiveness (RBE) of 211At for DSB induction varied between 1.4 and 3.1. A small increase of DSBs was observed in cycling cells compared to stationary cells. The repair kinetics was slower after 211At and more residual damage was found after 24 h. Comparison between cells with different proliferation status showed that the repair was inefficient in cycling cells with more residual damage, regardless of radiation quality. Activation of cell cycle arrests was investigated using immunofluorescent labeling of the checkpoint kinase Chk2 and by measuring cell cycle distributions with flow cytometry analysis. After alpha-particle irradiation, the average number of Chk2-foci was larger and the cells had a more affected cell cycle progression for several weeks compared with X-irradiated cells, indicating a more powerful arrest after 211At

  11. Main: PPD203 [AT Atlas

    Lifescience Database Archive (English)

    Full Text Available PPD203 PPD2 LED light for Green Fluorescent Protein (GFP) detection Development of ...innovative methods to support membrane protein crystallization Hiroaki Kato Graduate School of Pharmaceutical Sciences, Kyoto University PPD203.csml ...

  12. Final Report for research grant "Development of Methods for High Specific Activity Labeling of Biomolecules Using Astatine-211 in Different Oxidation States"

    Energy Technology Data Exchange (ETDEWEB)

    Wilbur, D. Scott [Univ. of Washington, Seattle, WA (United States)

    2011-12-14

    The overall objective of this research effort was to develop methods for labeling biomolecules with higher oxidation state species of At-211. This was to be done in an effort to develop reagents that had higher in vivo stability than the present carbon-bonded At-211-labeled compounds. We were unsuccessful in that effort, as none of the approaches studied provided reagents that were stable to in vivo deastatination. However, we gained a lot of information about At-211 in higher oxidation states. The studies proved to be very difficult as small changes in pH and other conditions appeared to change the nature of the species that obtained (by HPLC retention time analyses), with many of the species being unidentifiable. The fact that there are no stable isotopes of astatine, and the chemistry of the nearest halogen iodine is quite different, made it very difficult to interpret results of some experiments. With that said, we believe that a lot of valuable information was obtained from the studies. The research effort evaluated: (1) methods for chemical oxidation of At-211, (2) approaches to chelation of oxidized At-211, and (3) approaches to oxidation of astatophenyl compounds. A major hurdle that had to be surmounted to conduct the research was the development of HPLC conditions to separate and identify the various oxidized species formed. Attempts to develop conditions for separation of iodine and astatine species by normal and reversed-phase TLC and ITLC were not successful. However, we were successful in developing conditions (from a large number of attempts) to separate oxidized forms of iodine ([I-125]iodide, [I-125]iodate and [I-125]periodate) and astatine ([At-211]astatide, [At-211]astatate, [At-211]perastatate, and several unidentified At-211 species). Information on the basic oxidation and characterization of At-211 species is provided under Objective 1. Conditions were developed to obtain new At-211 labeling method where At-211 is chelated with the DOTA and

  13. 21 CFR 203.20 - Sales restrictions.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 4 2010-04-01 2010-04-01 false Sales restrictions. 203.20 Section 203.20 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS: GENERAL PRESCRIPTION DRUG MARKETING Sales Restrictions § 203.20 Sales restrictions. Except as provided in § 203.22 or...

  14. Reagents for Astatination of Biomolecules. 5. Evaluation of hydrazone linkers in 211At- and 125I-labeled closo-decaborate(2-) conjugates of Fab′ as a means of decreasing kidney retention

    Science.gov (United States)

    Wilbur, D. Scott; Chyan, Ming-Kuan; Hamlin, Donald K.; Nguyen, Holly; Vessella, Robert L.

    2011-01-01

    Evaluation of monoclonal antibody (MAb) fragments (e.g. Fab′, Fab or engineered fragments) as cancer-targeting reagents for therapy with the α-particle emitting radionuclide astatine-211 (211At) has been hampered by low in vivo stability of the label and a propensity of these proteins localize to kidneys. Fortunately, our group has shown that the low stability of the 211At label, generally a meta- or para-[211At]astatobenzoyl conjugate, on MAb Fab′ fragments can be dramatically improved by use of closo-decaborate(2-) conjugates. However, the higher stability of radiolabeled MAb Fab′ conjugates appears to result in retention of the radioactivity in kidneys. This investigation was conducted to evaluate whether the retention of radioactivity in kidney might be decreased by the use of acid-cleavable hydrazone between the Fab′ and the radiolabeled closo-decaborate(2-) moiety. Five conjugation reagents containing sulfhydryl-reactive maleimide groups, a hydrazone functionality and a closo-decaborate(2-) moiety were prepared. In four of the five conjugation reagents, a discrete polyethylene glycol (PEG) linker was used, and one substituent adjacent to the hydrazone was varied (phenyl, benzoate, anisole or methyl) to provide varying acid-sensitivity. In the initial studies, the five maleimido-closo-decaborate(2-) conjugation reagents were radioiodinated (125I or 131I), then conjugated with an anti-PSMA Fab′ (107-1A4 Fab′). Biodistributions of the five radioiodinated Fab′ conjugates were obtained in nude mice at 1, 4 and 24 h post injection (pi). In contrast to closo-decaborate(2-) conjugated to 107-1A4 Fab′ through a non-cleavable linker, two conjugates containing either a benzoate or a methyl substituent on the hydrazone functionality displayed clearance rates from kidney, liver and spleen that were similar to those obtained with directly radioiodinated Fab′ (i.e. no conjugate). The maleimido-closo-decaborate(2-) conjugation reagent containing a benzoate

  15. 40 CFR 240.203 - General design.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false General design. 240.203 Section 240.203 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES GUIDELINES FOR... design. ...

  16. 48 CFR 2825.203 - Evaluating offers.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Evaluating offers. 2825.203 Section 2825.203 Federal Acquisition Regulations System DEPARTMENT OF JUSTICE Socioeconomic Programs FOREIGN ACQUISITION Buy American Act-Construction Materials 2825.203 Evaluating offers. The HCA, or...

  17. 29 CFR 1614.203 - Rehabilitation Act.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 4 2010-07-01 2010-07-01 false Rehabilitation Act. 1614.203 Section 1614.203 Labor... EMPLOYMENT OPPORTUNITY Provisions Applicable to Particular Complaints § 1614.203 Rehabilitation Act. (a... Rehabilitation Act of 1973, as amended (29 U.S.C. 791), has been violated in a complaint alleging nonaffirmative...

  18. 24 CFR 203.673 - Habitability.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Habitability. 203.673 Section 203.673 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued... FAMILY MORTGAGE INSURANCE Servicing Responsibilities Occupied Conveyance § 203.673 Habitability. (a) For...

  19. 24 CFR 203.7 - Commitment process.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Commitment process. 203.7 Section 203.7 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued... Insurance, and Commitments § 203.7 Commitment process. For single family mortgage programs that are not...

  20. 47 CFR 97.203 - Beacon station.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Beacon station. 97.203 Section 97.203... SERVICE Special Operations § 97.203 Beacon station. (a) Any amateur station licensed to a holder of a Technician, Technician Plus, General, Advanced or Amateur Extra Class operator license may be a beacon. A...

  1. 22 CFR 203.1 - Purpose.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Purpose. 203.1 Section 203.1 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT REGISTRATION OF PRIVATE VOLUNTARY ORGANIZATIONS (PVOs) § 203.1... enable registered PVOs to make any representation to the public concerning the meaning of being...

  2. 21 CFR 131.203 - Lowfat yogurt.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Lowfat yogurt. 131.203 Section 131.203 Food and... CONSUMPTION MILK AND CREAM Requirements for Specific Standardized Milk and Cream § 131.203 Lowfat yogurt. (a) Description. Lowfat yogurt is the food produced by culturing one or more of the optional dairy ingredients...

  3. 42 CFR 37.203 - Autopsy specifications.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Autopsy specifications. 37.203 Section 37.203... EXAMINATIONS SPECIFICATIONS FOR MEDICAL EXAMINATIONS OF UNDERGROUND COAL MINERS Autopsies § 37.203 Autopsy specifications. (a) Every autopsy for which a claim for payment is submitted pursuant to this part: (1) Shall be...

  4. 1 CFR 20.3 - Organization.

    Science.gov (United States)

    2010-01-01

    ... 1 General Provisions 1 2010-01-01 2010-01-01 false Organization. 20.3 Section 20.3 General... DOCUMENTS HANDLING OF THE UNITED STATES GOVERNMENT MANUAL STATEMENTS § 20.3 Organization. (a) Information about lines of authority and organization may be reflected in a chart if the chart clearly delineates...

  5. 48 CFR 11.203 - Customer satisfaction.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Customer satisfaction. 11.203 Section 11.203 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION ACQUISITION PLANNING DESCRIBING AGENCY NEEDS Using and Maintaining Requirements Documents 11.203 Customer satisfaction...

  6. 50 CFR 223.203 - Anadromous fish.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Anadromous fish. 223.203 Section 223.203... Threatened Marine and Anadromous Species § 223.203 Anadromous fish. Available guidance documents cited in the...)(1) of the ESA (16 U.S.C. 1538(a)(1)) relating to endangered species apply to fish with an intact...

  7. 33 CFR 203.12 - Authority.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Authority. 203.12 Section 203.12... EMPLOYMENT OF ARMY AND OTHER RESOURCES, NATURAL DISASTER PROCEDURES Introduction § 203.12 Authority. Section.... The Secretary of the Army has delegated the authority vested in the Secretary under Public Law 84-99...

  8. 48 CFR 203.171-2 - Definition.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Definition. 203.171-2..., DEPARTMENT OF DEFENSE GENERAL IMPROPER BUSINESS PRACTICES AND PERSONAL CONFLICTS OF INTEREST Safeguards 203.171-2 Definition. Covered DoD official as used in this section, is defined in the clause at 252.203...

  9. 46 CFR 109.203 - Sanitation.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Sanitation. 109.203 Section 109.203 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS OPERATIONS Tests, Drills, and Inspections § 109.203 Sanitation. (a) The master or person in charge shall insure that the accommodation...

  10. 14 CFR 25.203 - Stall characteristics.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Stall characteristics. 25.203 Section 25.203 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY AIRPLANES Flight Stalls § 25.203 Stall characteristics. (a) It must...

  11. 30 CFR 75.203 - Mining methods.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Mining methods. 75.203 Section 75.203 Mineral... SAFETY STANDARDS-UNDERGROUND COAL MINES Roof Support § 75.203 Mining methods. (a) The method of mining... faulty pillar recovery methods. Pillar dimensions shall be compatible with effective control of the roof...

  12. 49 CFR 215.203 - Restricted cars.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Restricted cars. 215.203 Section 215.203..., DEPARTMENT OF TRANSPORTATION RAILROAD FREIGHT CAR SAFETY STANDARDS Restricted Equipment § 215.203 Restricted cars. (a) This section restricts the operation of any railroad freight car that is— (1) More than 50...

  13. 48 CFR 203.1004 - Contract clauses.

    Science.gov (United States)

    2010-10-01

    ... OF DEFENSE GENERAL IMPROPER BUSINESS PRACTICES AND PERSONAL CONFLICTS OF INTEREST Contractor Code of Business Ethics and Conduct 203.1004 Contract clauses. (a) Use the clause at 252.203-7003 in solicitations and contracts that include the FAR clause 52.203-13, Contractor Code of Business Ethics and Conduct...

  14. 41 CFR 50-203.18 - Evidence.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Evidence. 50-203.18 Section 50-203.18 Public Contracts and Property Management Other Provisions Relating to Public Contracts PUBLIC CONTRACTS, DEPARTMENT OF LABOR 203-RULES OF PRACTICE Minimum Wage Determinations Under the Walsh...

  15. 37 CFR 203.1 - General.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false General. 203.1 Section 203.1 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT OFFICE AND PROCEDURES FREEDOM OF INFORMATION ACT: POLICIES AND PROCEDURES Organization § 203.1 General. This information is...

  16. 24 CFR 203.443 - Insurance premium.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Insurance premium. 203.443 Section... premium. All of the provisions of §§ 203.260 through 203.269 1 concerning mortgage insurance premiums... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES SINGLE...

  17. Dicty_cDB: SLH203 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available SL (Link to library) SLH203 (Link to dictyBase) - - - Contig-U10734-1 SLH203Z (Link... to Original site) - - SLH203Z 683 - - - - Show SLH203 Library SL (Link to library) Clone ID SLH203 (Link to...ycdb.biol.tsukuba.ac.jp/CSM/SL/SLH2-A/SLH203Q.Seq.d/ Representative seq. ID SLH20...3Z (Link to Original site) Representative DNA sequence >SLH203 (SLH203Q) /CSM/SL/SLH2-A/SLH203Q.Seq.d/ XXXXX...ing significant alignments: (bits) Value SLH203 (SLH203Q) /CSM/SL/SLH2-A/SLH203Q.

  18. 12 CFR 268.203 - Rehabilitation Act.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Rehabilitation Act. 268.203 Section 268.203... Rehabilitation Act. (a) Model employer. The Board shall be a model employer of individuals with disabilities. The... Rehabilitation Act of 1973, as amended (29 U.S.C. 791), has been violated in a complaint alleging nonaffirmative...

  19. 48 CFR 203.170 - Business practices.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Business practices. 203..., DEPARTMENT OF DEFENSE GENERAL IMPROPER BUSINESS PRACTICES AND PERSONAL CONFLICTS OF INTEREST Safeguards 203.170 Business practices. To ensure the separation of functions for oversight, source selection...

  20. 48 CFR 370.203 - Definitions.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Definitions. 370.203... Opportunities 370.203 Definitions. For purposes of this Subpart 370.2, the following definitions shall apply: (a... Financing Act of 1974 (88 Stat. 77, 25 U.S.C. 1451). (d) Indian-owned economic enterprise means any Indian...

  1. 41 CFR 50-203.11 - Review.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Review. 50-203.11 Section...-Healey Public Contracts Act § 50-203.11 Review. (a) Within twenty (20) days after service of the decision... administrative law judge an original and four copies of a petition for review of the decision. The petition shall...

  2. 32 CFR 203.19 - Appeals process.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Appeals process. 203.19 Section 203.19 National....19 Appeals process. DoD Components will establish an appeals process to settle potential disputes... process. The Department of Defense recognizes that the RAB/TRC may disagree with the findings of the...

  3. 41 CFR 50-203.8 - Hearing.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Hearing. 50-203.8 Section 50-203.8 Public Contracts and Property Management Other Provisions Relating to Public Contracts..., examine, and cross-examine witnesses and to introduce into the record documentary or other evidence. (f...

  4. 7 CFR 1260.203 - Confidential treatment.

    Science.gov (United States)

    2010-01-01

    ... of persons subject to this subpart or statistical data collected therefrom, which statements do not... 7 Agriculture 10 2010-01-01 2010-01-01 false Confidential treatment. 1260.203 Section 1260.203 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING...

  5. 13 CFR 134.203 - The petition.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false The petition. 134.203 Section 134... BEFORE THE OFFICE OF HEARINGS AND APPEALS Rules of Practice for Most Cases § 134.203 The petition. (a) A petition must contain the following: (1) The basis of OHA's jurisdiction; (2) A copy of the SBA...

  6. 28 CFR 42.203 - Discrimination prohibited.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Discrimination prohibited. 42.203 Section...) of the Justice System Improvement Act of 1979 § 42.203 Discrimination prohibited. (a) No person in... participation in, be denied the benefits of, be subjected to discrimination under, or denied employment in...

  7. 5 CFR 302.203 - Disqualifying factors.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Disqualifying factors. 302.203 Section... IN THE EXCEPTED SERVICE Eligibility Standards § 302.203 Disqualifying factors. (a) The qualification... that certain reasons disqualify an applicant for appointment. The following, among others, may be...

  8. 40 CFR 86.203-94 - Abbreviations.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 18 2010-07-01 2010-07-01 false Abbreviations. 86.203-94 Section 86.203-94 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED... Later Model Year Gasoline-Fueled New Light-Duty Vehicles, New Light-Duty Trucks and New Medium-Duty...

  9. 33 CFR 135.203 - Amount required.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Amount required. 135.203 Section 135.203 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE POLLUTION FINANCIAL RESPONSIBILITY AND COMPENSATION OFFSHORE OIL POLLUTION COMPENSATION FUND...

  10. 32 CFR 203.6 - Cost principles.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Cost principles. 203.6 Section 203.6 National... Cost principles. (a) Non-profit contractors must comply with the cost principles in OMB Circular A-122...) For-profit contractors and subcontractors must comply with the cost principles in the FAR (48 CFR part...

  11. 31 CFR 203.17 - Collector depositaries.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Collector depositaries. 203.17 Section 203.17 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE PAYMENT OF FEDERAL TAXES AND THE TREASURY...

  12. 31 CFR 203.1 - Scope.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Scope. 203.1 Section 203.1 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE PAYMENT OF FEDERAL TAXES AND THE TREASURY TAX AND LOAN PROGRAM...

  13. 47 CFR 18.203 - Equipment authorization.

    Science.gov (United States)

    2010-10-01

    ... information already on file with the Commission. (2) A technical report pursuant to §§ 18.207 and 18.311. (b... 47 Telecommunication 1 2010-10-01 2010-10-01 false Equipment authorization. 18.203 Section 18.203 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL INDUSTRIAL, SCIENTIFIC, AND MEDICAL EQUIPMENT...

  14. 42 CFR 93.203 - Complainant.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Complainant. 93.203 Section 93.203 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES HEALTH ASSESSMENTS AND HEALTH EFFECTS STUDIES OF HAZARDOUS SUBSTANCES RELEASES AND FACILITIES PUBLIC HEALTH SERVICE POLICIES ON RESEARCH...

  15. 37 CFR 203.3 - Organization.

    Science.gov (United States)

    2010-07-01

    ... 203.3 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT OFFICE AND PROCEDURES FREEDOM OF INFORMATION ACT: POLICIES AND PROCEDURES Organization § 203.3 Organization. (a) In... registration that are not selected by the Library of Congress for addition to its collections. (5) The...

  16. 27 CFR 24.203 - Honey wine.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Honey wine. 24.203 Section... THE TREASURY LIQUORS WINE Production of Agricultural Wine § 24.203 Honey wine. (a) Subject to paragraph (b) of this section, a winemaker, in the production of wine from honey, may add the following: (1...

  17. 24 CFR 3285.203 - Site Drainage.

    Science.gov (United States)

    2010-04-01

    ... per foot away from the foundation for the first ten feet. Where property lines, walls, slopes, or... of foundation supports and to prevent water build-up under the home, as shown in Figure to § 3285.203...

  18. 33 CFR 203.13 - Available assistance.

    Science.gov (United States)

    2010-07-01

    ... DEFENSE EMERGENCY EMPLOYMENT OF ARMY AND OTHER RESOURCES, NATURAL DISASTER PROCEDURES Introduction § 203... must be justified from an engineering and economic standpoint, and must be capable of completion in a...

  19. 48 CFR 1631.203 - Indirect costs.

    Science.gov (United States)

    2010-10-01

    ... Section 1631.203 Federal Acquisition Regulations System OFFICE OF PERSONNEL MANAGEMENT FEDERAL EMPLOYEES HEALTH BENEFITS ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND... general practice in the insurance industry. [70 FR 31391, June 1, 2005] ...

  20. 24 CFR 203.42 - Rental properties.

    Science.gov (United States)

    2010-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES SINGLE... rented or offered for rent for transient or hotel purposes, as defined in § 203.16, so long as the...

  1. 49 CFR 399.203 - Applicability.

    Science.gov (United States)

    2010-10-01

    ... STANDARDS Step, Handhold, and Deck Requirements for Commercial Motor Vehicles § 399.203 Applicability. This subpart applies to all trucks and truck-tractors, having a high profile cab-over-engine (COE...

  2. 5 CFR 2606.203 - Granting access.

    Science.gov (United States)

    2010-01-01

    ... Access to Records and Accounting of Disclosures § 2606.203 Granting access. (a) The methods for allowing... documentation will be required for the disclosure to the data subject of information required to be made...

  3. 7 CFR 3430.203 - Eligibility.

    Science.gov (United States)

    2010-01-01

    ... Regulations of the Department of Agriculture (Continued) COOPERATIVE STATE RESEARCH, EDUCATION, AND EXTENSION..., national laboratories; colleges and universities (offering associate's or higher degrees); research...-GENERAL AWARD ADMINISTRATIVE PROVISIONS Specialty Crop Research Initiative § 3430.203 Eligibility...

  4. 23 CFR 230.203 - Policy.

    Science.gov (United States)

    2010-04-01

    ..., Disadvantaged, and Women Business Enterprises § 230.203 Policy. Based on the provisions of Pub. L. 97-424, dated... participation of minority business enterprises in Federal-aid highway contracts in part through the development...

  5. 24 CFR 51.203 - Safety standards.

    Science.gov (United States)

    2010-04-01

    ... Conventional Fuels or Chemicals of an Explosive or Flammable Nature § 51.203 Safety standards. The following... facilities or areas shall not exceed 0.5 psi. (c) If a hazardous substance constitutes both a thermal...

  6. 48 CFR 203.1003 - Requirements.

    Science.gov (United States)

    2010-10-01

    ... OF DEFENSE GENERAL IMPROPER BUSINESS PRACTICES AND PERSONAL CONFLICTS OF INTEREST Contractor Code of Business Ethics and Conduct 203.1003 Requirements. (b) Notification of possible contractor violation. Upon...

  7. 41 CFR 50-203.17 - Hearings.

    Science.gov (United States)

    2010-07-01

    ... PUBLIC CONTRACTS, DEPARTMENT OF LABOR 203-RULES OF PRACTICE Minimum Wage Determinations Under the Walsh... with regard to prevailing minimum wages in the various industries shall be conducted by an...

  8. 24 CFR 203.259a - Scope.

    Science.gov (United States)

    2010-04-01

    ... MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES SINGLE FAMILY MORTGAGE INSURANCE Contract Rights and Obligations Mortgage Insurance Premiums-in General § 203.259a Scope... Insurance Premiums—Periodic Payment ...

  9. 24 CFR 203.203 - Issuance and nature of insured 10-year protection plans.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Issuance and nature of insured 10... Underwriting Procedures Insured Ten-Year Protection Plans (plan) § 203.203 Issuance and nature of insured 10-year protection plans. (a) Plans may be issued: (1) By a builder, warranty company, insurance company...

  10. 48 CFR 828.203 - Acceptability of individual sureties.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Acceptability of individual sureties. 828.203 Section 828.203 Federal Acquisition Regulations System DEPARTMENT OF VETERANS... Acceptability of individual sureties. ...

  11. 41 CFR 50-203.15 - Initiation of proceeding.

    Science.gov (United States)

    2010-07-01

    ... Public Contracts PUBLIC CONTRACTS, DEPARTMENT OF LABOR 203-RULES OF PRACTICE Minimum Wage Determinations Under the Walsh-Healey Public Contracts Act § 50-203.15 Initiation of proceeding. Wage determination...

  12. MISSING MATTHEW'S MEANING. WITH 'TEXTUAL MEANING' 203 ...

    African Journals Online (AJOL)

    Stellenbosch Papers in Linguistics, Vol. 25, 1992, 203-235 .... in here is not a theory that will work well only in the less ... (Gutt's distinction between primary and secondary ... sion which I have just quoted from is both too long and too technical.

  13. 48 CFR 33.203 - Applicability.

    Science.gov (United States)

    2010-10-01

    ... in paragraph (b) below, this part applies to any express or implied contract covered by the Federal Acquisition Regulation. (b) This subpart does not apply to any contract with (1) a foreign government or... Section 33.203 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING...

  14. 15 CFR 970.203 - Exploration plan.

    Science.gov (United States)

    2010-01-01

    ... DATA SERVICE DEEP SEABED MINING REGULATIONS FOR EXPLORATION LICENSES Applications Contents § 970.203...; (iii) Designing and testing system components onshore and at sea; (iv) Designing and testing mining... in the area which will affect nodule recovery system design and operations (e.g., seafloor topography...

  15. 5 CFR 340.203 - Technical assistance.

    Science.gov (United States)

    2010-01-01

    ... part-time employment practices; (3) Development of special recruitment and selection techniques for...-TIME CAREER EMPLOYMENT (PART-TIME, SEASONAL, ON-CALL, AND INTERMITTENT) Regulatory Requirements-Part-Time Employment § 340.203 Technical assistance. (a) The Office of Personnel Management shall provide...

  16. 48 CFR 50.203 - General.

    Science.gov (United States)

    2010-10-01

    ... that will enhance the protection of the nation; and (2) Provide risk management and litigation management protections for sellers of QATTs and others in the supply and distribution chain. (b) The SAFETY....203 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT...

  17. 48 CFR 52.203-3 - Gratuities.

    Science.gov (United States)

    2010-10-01

    ... as in a breach of the contract; and (2) In addition to any other damages provided by law, to... AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 52.203-3...) Intended, by the gratuity, to obtain a contract or favorable treatment under a contract. (b) The facts...

  18. 31 CFR 203.2 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... LOAN PROGRAM General Information § 203.2 Definitions. Advice of credit (AOC) means the paper or..., regulations, and instructions. Balance limit means the highest amount a depositary has stated it will accept... investments in its TIP main account balance and/or its Special Direct Investment (SDI) account balance. With...

  19. 21 CFR 203.3 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... PRESCRIPTION DRUG MARKETING General Provisions § 203.3 Definitions. (a) The act means the Federal Food, Drug..., data, audio, pictorial, or other information representation in digital form that is created, modified... same marketing or service area, or nearby licensed practitioners, of drugs for use in the treatment of...

  20. 43 CFR 17.203 - Discrimination prohibited.

    Science.gov (United States)

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Discrimination prohibited. 17.203 Section... Discrimination prohibited. (a) General. No qualified handicapped person shall, on the basis of handicap, be excluded from participation in, be denied the benefits of, or otherwise be subjected to discrimination...

  1. 5 CFR 2635.203 - Definitions.

    Science.gov (United States)

    2010-01-01

    .... (c) Market value means the retail cost the employee would incur to purchase the gift. An employee who... OF THE EXECUTIVE BRANCH Gifts From Outside Sources § 2635.203 Definitions. For purposes of this... department determines exercises distinct and separate functions. (b) Gift includes any gratuity, favor...

  2. 5 CFR 2640.203 - Miscellaneous exemptions.

    Science.gov (United States)

    2010-01-01

    ... Section 2640.203 Administrative Personnel OFFICE OF GOVERNMENT ETHICS GOVERNMENT ETHICS INTERPRETATION... prosecute a case involving the fraudulent practices of an issuer of junk bonds. While developing the facts... interest in the medical product or device is purchase of it for use by, or sale to, its patients; or (2...

  3. 5 CFR 250.203 - Agency responsibilities.

    Science.gov (United States)

    2010-01-01

    ... management on its overall mission performance. (2) Human capital accountability system. This system provides... MANAGEMENT IN AGENCIES Strategic Human Capital Management § 250.203 Agency responsibilities. (a) To assist in... and initiatives as appropriate, consistent with agency strategic plans and annual performance goals...

  4. 18 CFR 1304.203 - Vegetation management.

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false Vegetation management...-Owned Residential Access Shoreland § 1304.203 Vegetation management. No vegetation management shall be approved on TVA-owned Residential Access Shoreland until a Vegetation Management Plan meeting the...

  5. 41 CFR 50-203.21 - Decisions.

    Science.gov (United States)

    2010-07-01

    ... PUBLIC CONTRACTS, DEPARTMENT OF LABOR 203-RULES OF PRACTICE Minimum Wage Determinations Under the Walsh..., and (2) any proposed wage determination. Any tentative decision shall be published in the Federal... wage determination. Any final decision shall be published in the Federal Register. [26 FR 8945, Sept...

  6. 7 CFR 1220.203 - Nominations.

    Science.gov (United States)

    2010-01-01

    ... CONSUMER INFORMATION Soybean Promotion and Research Order United Soybean Board § 1220.203 Nominations. All... be filled. (e) Nominations may be submitted in order of preference and for the initial Board, in order of preference for staggered terms. Should the Secretary reject any nomination submitted and there...

  7. 48 CFR 1815.203-72 - Risk management.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Risk management. 1815.203-72 Section 1815.203-72 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE... Proposals and Information 1815.203-72 Risk management. In all RFPs and RFOs for supplies or services for...

  8. 32 CFR 48.203 - Election of options.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 1 2010-07-01 2010-07-01 false Election of options. 48.203 Section 48.203 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE PERSONNEL, MILITARY AND CIVILIAN RETIRED SERVICEMAN'S FAMILY PROTECTION PLAN Election of Options § 48.203 Election of options. (a) A member...

  9. 46 CFR 203.5 - Types of hearings.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false Types of hearings. 203.5 Section 203.5 Shipping MARITIME... CERTAIN HEARINGS UNDER THE MERCHANT MARINE ACT, 1936, AS AMENDED § 203.5 Types of hearings. (a) Oral... seeking the oral evidentiary hearing. (b) Hearing on Submission of Written Evidence and Argument: If, upon...

  10. 24 CFR 203.39 - Standards for buildings.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Standards for buildings. 203.39 Section 203.39 Housing and Urban Development Regulations Relating to Housing and Urban Development... § 203.39 Standards for buildings. The buildings on the mortgaged property must conform with the...

  11. 5 CFR 551.203 - Salary-based nonexemption.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Salary-based nonexemption. 551.203 Section 551.203 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS PAY ADMINISTRATION UNDER THE FAIR LABOR STANDARDS ACT Exemptions and Exclusions § 551.203 Salary-based nonexemption...

  12. 24 CFR 203.18a - Solar energy system.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Solar energy system. 203.18a... § 203.18a Solar energy system. (a) The dollar limitation provided in § 203.18(a) may be increased by up... to the installation of a solar energy system. (b) Solar energy system is defined as any addition...

  13. 5 CFR 2601.203 - Conflict of interest analysis.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Conflict of interest analysis. 2601.203 Section 2601.203 Administrative Personnel OFFICE OF GOVERNMENT ETHICS ORGANIZATION AND PROCEDURES... Acceptance of Gifts § 2601.203 Conflict of interest analysis. (a) A gift shall not be solicited or accepted...

  14. 9 CFR 113.203 - Feline Panleukopenia Vaccine, Killed Virus.

    Science.gov (United States)

    2010-01-01

    ... Virus. 113.203 Section 113.203 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS STANDARD REQUIREMENTS Killed Virus Vaccines § 113.203 Feline Panleukopenia Vaccine, Killed Virus. Feline Panleukopenia...

  15. 48 CFR 916.203-4 - Contract clauses.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contract clauses. 916.203-4 Section 916.203-4 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Fixed-Price Contracts 916.203-4 Contract clauses. (d)(2) The...

  16. 20 CFR 203.7 - Local lodge employee.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Local lodge employee. 203.7 Section 203.7... THE ACT § 203.7 Local lodge employee. An individual who, prior to January 1, 1937, shall have rendered service to a local lodge or division of a railway labor organization included as an employer under section...

  17. 48 CFR 32.203 - Determining contract financing terms.

    Science.gov (United States)

    2010-10-01

    ... financing terms. 32.203 Section 32.203 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING Commercial Item Purchase Financing 32.203 Determining contract financing terms. When the criteria in 32.202-1(b) are met, the contracting officer may either...

  18. 31 CFR 203.19 - Sources of balances.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Sources of balances. 203.19 Section 203.19 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL... § 203.19 Sources of balances. A financial institution must be a collector depositary that accepts term...

  19. 42 CFR 57.203 - Application by school.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Application by school. 57.203 Section 57.203 Public... Loans § 57.203 Application by school. (a) Each school seeking a Federal capital contribution must submit... Secretary and the applicant school for a Federal capital contribution under section 721 of the Act is...

  20. 30 CFR 203.53 - What relief will MMS grant?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What relief will MMS grant? 203.53 Section 203.53 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR MINERALS REVENUE...-Life Leases § 203.53 What relief will MMS grant? (a) If we approve your application and you meet...

  1. Production of 203Pb-tris-hydroxymethyl amino methane

    International Nuclear Information System (INIS)

    Lambrecht, R.M.; Packer, S.; Merrill, J.C.; Atkins, H.L.; Wolf, A.P.; Bradley-Moore, P.R.

    1977-01-01

    203 Pb-tris complex is injected for use in the detection and localization of tumors. The lead-203 is produced from the deuteron bombardment of a thallium target and chemically separated from the thallium. The tris is added which complexes with the lead-203

  2. 28 CFR 55.6 - Coverage under section 203(c).

    Science.gov (United States)

    2010-07-01

    ... THE VOTING RIGHTS ACT REGARDING LANGUAGE MINORITY GROUPS Nature of Coverage § 55.6 Coverage under section 203(c). (a) Coverage formula. There are four ways in which a political subdivision can become subject to section 203(c). 2 2 The criteria for coverage are contained in section 203(b). (1) Political...

  3. 37 CFR 203.5 - Inspection and copying.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Inspection and copying. 203.5 Section 203.5 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT OFFICE AND PROCEDURES FREEDOM OF INFORMATION ACT: POLICIES AND PROCEDURES Availability of Information § 203.5...

  4. Tank 241-U-203: Tank Characterization Plan

    International Nuclear Information System (INIS)

    Sathyanarayana, P.

    1995-01-01

    The revised Federal Facility Agreement and Consent Order states that a tank characterization plan will be developed for each double-shell tank and single-shell tank using the data quality objective process. The plans are intended to allow users and regulators to ensure their needs will be met and resources are devoted to gaining only necessary information. This document satisfies that requirement for Tank 241-U-203 sampling activities

  5. 48 CFR 570.203-3 - Soliciting offers.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Soliciting offers. 570.203... 570.203-3 Soliciting offers. (a) Solicit offers by providing each prospective offeror a proposed short..., evaluation procedures and submissions of offers. ...

  6. 20 CFR 701.202-701.203 - [Reserved

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false [Reserved] 701.202-701.203 Section 701.202-701.203 Employees' Benefits EMPLOYMENT STANDARDS ADMINISTRATION, DEPARTMENT OF LABOR LONGSHOREMEN'S AND HARBOR WORKERS' COMPENSATION ACT AND RELATED STATUTES GENERAL; ADMINISTERING AGENCY; DEFINITIONS...

  7. 24 CFR 983.203 - HAP contract information.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false HAP contract information. 983.203... DEVELOPMENT PROJECT-BASED VOUCHER (PBV) PROGRAM Housing Assistance Payments Contract § 983.203 HAP contract information. The HAP contract must specify: (a) The total number of contract units by number of bedrooms; (b...

  8. 7 CFR 400.203 - Financial statement and certification.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Financial statement and certification. 400.203 Section...-Standards for Approval § 400.203 Financial statement and certification. (a) An entity desiring to become or continue as a contractor shall submit to the Corporation a financial statement which is as of a date not...

  9. 22 CFR 203.11 - Access to records and communications.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Access to records and communications. 203.11 Section 203.11 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT REGISTRATION OF PRIVATE VOLUNTARY... documents that are made available to USAID pursuant to this regulation must be made available for public...

  10. 48 CFR 1328.203 - Acceptability of individual sureties.

    Science.gov (United States)

    2010-10-01

    ... individual sureties. 1328.203 Section 1328.203 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE... Acceptability of individual sureties. (a) Contracting officers shall obtain the opinion of the Procurement Counsel as to the adequacy of the documents pledging the assets of an individual surety prior to accepting...

  11. 48 CFR 1328.203-7 - Exclusion of individual sureties.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Exclusion of individual sureties. 1328.203-7 Section 1328.203-7 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE... Exclusion of individual sureties. The designee authorized to exclude an individual from acting as a surety...

  12. 48 CFR 28.203 - Acceptability of individual sureties.

    Science.gov (United States)

    2010-10-01

    ... individual sureties. 28.203 Section 28.203 Federal Acquisition Regulations System FEDERAL ACQUISITION... Acceptability of individual sureties. (a) An individual surety is acceptable for all types of bonds except position schedule bonds. The contracting officer shall determine the acceptability of individuals proposed...

  13. 42 CFR 400.203 - Definitions specific to Medicaid.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 2 2010-10-01 2010-10-01 false Definitions specific to Medicaid. 400.203 Section 400.203 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN... Medicaid. As used in connection with the Medicaid program, unless the context indicates otherwise...

  14. 19 CFR 201.203 - Delegation of authority.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 3 2010-04-01 2010-04-01 false Delegation of authority. 201.203 Section 201.203 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION GENERAL RULES OF GENERAL APPLICATION Debt... accordance with the policies contained herein and as otherwise provided by law. ...

  15. 46 CFR 16.203 - Employer, MRO, and SAP responsibilities.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Employer, MRO, and SAP responsibilities. 16.203 Section... CHEMICAL TESTING Required Chemical Testing § 16.203 Employer, MRO, and SAP responsibilities. (a) Employers...) Substance Abuse Professional (SAP). Individuals performing SAP functions must meet the training requirements...

  16. 24 CFR 203.512 - Free assumability; exceptions.

    Science.gov (United States)

    2010-04-01

    ... AUTHORITIES SINGLE FAMILY MORTGAGE INSURANCE Servicing Responsibilities General Requirements § 203.512 Free..., agree to or enforce legal restrictions on conveyance, as defined in § 203.41(a)(3) of this part, or... descent. (c) Investors and secondary residences. The mortgagee shall not approve the sale of other...

  17. 31 CFR 203.21 - Collateral security requirements.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Collateral security requirements. 203.21 Section 203.21 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued... hereunder; or (iv) The depositary is closed for business by regulatory action or by proper corporate action...

  18. 49 CFR 384.203 - Driving while under the influence.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false Driving while under the influence. 384.203 Section... § 384.203 Driving while under the influence. (a) The State must have in effect and enforce through... apply its criminal or other sanctions for driving under the influence to a person found to have operated...

  19. 24 CFR 203.20 - Agreed interest rate.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Agreed interest rate. 203.20... § 203.20 Agreed interest rate. (a) The mortgage shall bear interest at the rate agreed upon by the mortgagee and the mortgagor. (b) Interest shall be payable in monthly installments on the principal amount...

  20. 27 CFR 20.203 - Losses on premises.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Losses on premises. 20.203... OF THE TREASURY LIQUORS DISTRIBUTION AND USE OF DENATURED ALCOHOL AND RUM Losses § 20.203 Losses on premises. (a) Recording of losses. A permittee shall determine and record, in the records prescribed by...

  1. 28 CFR 16.203 - Closed meetings-Formal procedure.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Closed meetings-Formal procedure. 16.203 Section 16.203 Judicial Administration DEPARTMENT OF JUSTICE PRODUCTION OR DISCLOSURE OF MATERIAL OR... involvement of any person in the commission of a crime, but only to the extent that the production of such...

  2. 48 CFR 1519.203 - Mentor-protege.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Mentor-protege. 1519.203... PROGRAMS SMALL BUSINESS PROGRAMS Policies 1519.203 Mentor-protege. (a) The Contracting officer shall insert the clause at 1552.219-70, Mentor-Protege Program, in all contracts under which the Contractor has...

  3. 31 CFR 203.10 - Electronic payment methods.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Electronic payment methods. 203.10... TAX AND LOAN PROGRAM Electronic Federal Tax Payments § 203.10 Electronic payment methods. (a) General. Electronic payment methods for Federal tax payments available under this subpart include ACH debit entries...

  4. 41 CFR 109-38.203 - Agency identification.

    Science.gov (United States)

    2010-07-01

    ... Administrative Services, Logistics Management Division, Headquarters, using DOE Form 4250.2, “Requisition for... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Agency identification. 109-38.203 Section 109-38.203 Public Contracts and Property Management Federal Property Management...

  5. 14 CFR 139.203 - Contents of Airport Certification Manual.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Contents of Airport Certification Manual. 139.203 Section 139.203 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... succession of airport operational responsibility X X X X 2. Each current exemption issued to the airport from...

  6. 31 CFR 203.14 - Scope of the subpart.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Scope of the subpart. 203.14 Section 203.14 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE PAYMENT OF FEDERAL TAXES AND THE TREASURY...

  7. 31 CFR 203.9 - Scope of the subpart.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Scope of the subpart. 203.9 Section 203.9 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE PAYMENT OF FEDERAL TAXES AND THE TREASURY TAX AND...

  8. 31 CFR 203.3 - TT&L depositaries.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false TT&L depositaries. 203.3 Section 203.3 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE PAYMENT OF FEDERAL TAXES AND THE TREASURY TAX AND...

  9. 31 CFR 203.15 - Tax deposits using FTD coupons.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Tax deposits using FTD coupons. 203.15 Section 203.15 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE PAYMENT OF FEDERAL TAXES AND THE...

  10. 41 CFR 50-203.22 - Effective date of determinations.

    Science.gov (United States)

    2010-07-01

    ... Public Contracts PUBLIC CONTRACTS, DEPARTMENT OF LABOR 203-RULES OF PRACTICE Minimum Wage Determinations Under the Walsh-Healey Public Contracts Act § 50-203.22 Effective date of determinations. Any minimum wage determination issued as a result of hearings held under this subpart shall take effect not less...

  11. 24 CFR 203.38 - Location of dwelling.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Location of dwelling. 203.38... § 203.38 Location of dwelling. At the time a mortgage is insured there must be located on the mortgaged property one or more dwellings designed principally for residential use for not more than four families...

  12. 37 CFR 203.2 - Authority and functions.

    Science.gov (United States)

    2010-07-01

    ....2 Section 203.2 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT OFFICE AND PROCEDURES FREEDOM OF INFORMATION ACT: POLICIES AND PROCEDURES Organization § 203.2 Authority and functions. (a) The administration of the copyright law was entrusted to the Library of Congress by...

  13. 37 CFR 203.4 - Methods of operation.

    Science.gov (United States)

    2010-07-01

    ... Section 203.4 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT OFFICE AND PROCEDURES FREEDOM OF INFORMATION ACT: POLICIES AND PROCEDURES Procedures § 203.4 Methods of... refusals to register claims to copyright; statements of policy and interpretations which have been adopted...

  14. Draft genome sequence of the Coccolithovirus Emiliania huxleyi virus 203.

    Science.gov (United States)

    Nissimov, Jozef I; Worthy, Charlotte A; Rooks, Paul; Napier, Johnathan A; Kimmance, Susan A; Henn, Matthew R; Ogata, Hiroyuki; Allen, Michael J

    2011-12-01

    The Coccolithoviridae are a recently discovered group of viruses that infect the marine coccolithophorid Emiliania huxleyi. Emiliania huxleyi virus 203 (EhV-203) has a 160- to 180-nm-diameter icosahedral structure and a genome of approximately 400 kbp, consisting of 464 coding sequences (CDSs). Here we describe the genomic features of EhV-203 together with a draft genome sequence and its annotation, highlighting the homology and heterogeneity of this genome in comparison with the EhV-86 reference genome.

  15. 24 CFR 203.264 - Payment of periodic MIP.

    Science.gov (United States)

    2010-04-01

    ... Payment § 203.264 Payment of periodic MIP. The mortgagee shall pay each MIP in twelve equal monthly installments. Each monthly installment shall be due and payable to the Commissioner no later than the tenth day...

  16. Soil moisture determination with Tesla NZK 203 neutron gage

    International Nuclear Information System (INIS)

    Hally, J.

    1977-01-01

    Soil moisture was measured using the NZK 203 neutron probe manufactured by Tesla Premysleni. The individual measuring sites were spaced at a distance of 100 m. The NZK 203 set consists of a NPK 202 moisture gage and a NSK 301 scintillation detector and features the following specifications: moisture density measuring range 20 to 500 kg/m 3 , 241 Am-Be fast neutron source having a neutron flux of 7.5x10 4 n.sec -1 +-10%, operating temperature -10 to +45 degC. The measured counting rate was primarily affected by the statistical fluctuation of ionizing radiation and by instrument instability. In order that these effects should be limited each measurement was repeated 10 times with the optimum measurement time at an interval of 20 to 100 sec. The NZK 203 Tesla set was proven to be suitable for rapid and reproducible determination of moisture profiles. (J.P.)

  17. Tank 241-B-203 push mode core sampling and analysis plan. Revision 1

    International Nuclear Information System (INIS)

    Jo, J.

    1995-01-01

    This Sampling and Analysis Plan (SAP) identifies characterization objectives pertaining to sample collection, laboratory analytical evaluation, and reporting requirements for two push-mode core samples from tank 241-B-203 (B-203)

  18. 30 CFR 203.75 - What risk do I run if I request a redetermination?

    Science.gov (United States)

    2010-07-01

    ... Relief for Pre-Act Deep Water Leases and for Development and Expansion Projects § 203.75 What risk do I... evaluate your application under § 203.67 using the conditions prevailing at the time of your...

  19. 45 CFR 150.203 - Circumstances requiring CMS enforcement.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 1 2010-10-01 2010-10-01 false Circumstances requiring CMS enforcement. 150.203... CARE ACCESS CMS ENFORCEMENT IN GROUP AND INDIVIDUAL INSURANCE MARKETS CMS Enforcement Processes for... requiring CMS enforcement. CMS enforces HIPAA requirements to the extent warranted (as determined by CMS) in...

  20. 24 CFR 203.385 - Types of satisfactory title evidence.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Types of satisfactory title... Title Waivers § 203.385 Types of satisfactory title evidence. The following types of title evidence shall be satisfactory to the Commissioner: (a) Fee or owner's title policy. A fee or owner's policy of...

  1. 24 CFR 1003.203 - Special economic development activities.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Special economic development... Eligible Activities § 1003.203 Special economic development activities. A grantee may use ICDBG funds for special economic development activities in addition to other activities authorized in this subpart which...

  2. 24 CFR 570.203 - Special economic development activities.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 3 2010-04-01 2010-04-01 false Special economic development... § 570.203 Special economic development activities. A recipient may use CDBG funds for special economic... part of an economic development project. Guidelines for selecting activities to assist under this...

  3. 46 CFR 199.203 - Marshalling of liferafts.

    Science.gov (United States)

    2010-10-01

    ... LIFESAVING SYSTEMS FOR CERTAIN INSPECTED VESSELS Additional Requirements for Passenger Vessels § 199.203 Marshalling of liferafts. (a) Each passenger vessel must have a lifeboat or rescue boat for each six liferafts when— (1) Each lifeboat and rescue boat is loaded with its full complement of persons; and (2) The...

  4. 31 CFR 29.203 - Service of Process.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Service of Process. 29.203 Section 29... Service of Process. To affect Federal Benefit Payments— (a) Service must be made upon the Department at the address provided in appendix A to this subpart for— (1) Legal process under section 659 of title...

  5. 48 CFR 43.203 - Change order accounting procedures.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Change order accounting... CONTRACT MANAGEMENT CONTRACT MODIFICATIONS Change Orders 43.203 Change order accounting procedures. (a) Contractors' accounting systems are seldom designed to segregate the costs of performing changed work...

  6. 27 CFR 46.203 - Record (book) inventory requirements.

    Science.gov (United States)

    2010-04-01

    ... quantities of articles actually on hand as if a physical inventory had taken place on April 1, 2009. See the... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Record (book) inventory... Cigarette Tubes Held for Sale on April 1, 2009 Inventories § 46.203 Record (book) inventory requirements. (a...

  7. 18 CFR 701.203 - Schedule of fees.

    Science.gov (United States)

    2010-04-01

    ... by the Public Information Officer on the basis of the balance between the benefit to the general public and the cost to the Water Resources Council. (d) Fees shall be limited to recovery of only direct... ORGANIZATION Availability of Information § 701.203 Schedule of fees. (a) The Public Information Officer will to...

  8. 48 CFR 828.203-7 - Exclusion of individual sureties.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Exclusion of individual... Exclusion of individual sureties. The DSPE may make the determinations referenced in FAR 28.203-7: (a) To exclude individuals from acting as surety on bonds; and (b) To accept bonds from individuals named on the...

  9. 24 CFR 203.353 - Certification by mortgagee.

    Science.gov (United States)

    2010-04-01

    ... SINGLE FAMILY MORTGAGE INSURANCE Contract Rights and Obligations Assignment of Mortgage § 203.353... Commissioner that: (a) Priority of mortgage to liens. The mortgage is prior to all mechanics' and materialmen's liens filed of record, regardless of when such liens attach, and prior to all liens and encumbrances, or...

  10. 48 CFR 1329.203-70 - DOC Federal tax exemption.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false DOC Federal tax exemption... CONTRACTING REQUIREMENTS TAXES Federal Excise Taxes 1329.203-70 DOC Federal tax exemption. (a) The Office of... enabling DOC and its contractors to purchase spirits (e.g., specially denatured spirits) tax-free for non...

  11. 41 CFR 50-203.20 - Examination of witnesses.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Examination of witnesses... Under the Walsh-Healey Public Contracts Act § 50-203.20 Examination of witnesses. The administrative law... such examination or cross-examination of any witness as may be required for a full and true disclosure...

  12. 31 CFR 203.16 - Retainer and investor depositaries.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Retainer and investor depositaries... TREASURY TAX AND LOAN PROGRAM PATAX § 203.16 Retainer and investor depositaries. (a) Credit to TIP main account balance. On the business day that the TSC receives an AOC from a retainer or investor depositary...

  13. 5 CFR 410.203 - Options for developing employees.

    Science.gov (United States)

    2010-01-01

    ...-development activities, coaching, mentoring, career development counseling, details, rotational assignments... Planning and Evaluating Training § 410.203 Options for developing employees. Agencies may use a full range of options to meet their mission-related organizational and employee development needs, such as...

  14. 40 CFR 94.203 - Application for certification.

    Science.gov (United States)

    2010-07-01

    ... (CONTINUED) CONTROL OF EMISSIONS FROM MARINE COMPRESSION-IGNITION ENGINES Certification Provisions § 94.203 Application for certification. (a) For each engine family that complies with all applicable standards and... application of the engine (e.g., used to propel planing vessels, use to propel vessels with variable-pitch...

  15. 24 CFR 203.405 - Debenture interest rate.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Debenture interest rate. 203.405... Debenture interest rate. (a) Debentures shall bear interest from the date of issue, payable semiannually on... claim is paid in cash, the debenture interest rate for purposes of calculating such a claim shall be the...

  16. 24 CFR 203.479 - Debenture interest rate.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Debenture interest rate. 203.479... interest rate. (a) Debentures shall bear interest from the date of issue, payable semiannually on the first... applicable rates of interest will be published twice each year as a notice in the Federal Register. (b) For...

  17. 42 CFR 405.203 - FDA categorization of investigational devices.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 2 2010-10-01 2010-10-01 false FDA categorization of investigational devices. 405... Coverage Decisions That Relate to Health Care Technology § 405.203 FDA categorization of investigational.../investigational (Category A) or non-experimental/investigational (Category B). (c) CMS uses the categorization of...

  18. 41 CFR 101-27.203 - Program objectives.

    Science.gov (United States)

    2010-07-01

    ...-Management of Shelf-Life Materials § 101-27.203 Program objectives. In order to assure maximum use of shelf...) Conduct inventory management analyses to determine if shelf-life stocks are expected to be utilized prior... transfer of such stock in sufficient time to permit usage prior to deterioration. (e) Make available for...

  19. 14 CFR 125.203 - Communication and navigation equipment.

    Science.gov (United States)

    2010-01-01

    ... within the degree of accuracy required for ATC; (ii) One marker beacon receiver providing visual and... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Communication and navigation equipment. 125... Equipment Requirements § 125.203 Communication and navigation equipment. (a) Communication equipment—general...

  20. 30 CFR 90.203 - Certified person; maintenance and calibration.

    Science.gov (United States)

    2010-07-01

    ... COAL MINE SAFETY AND HEALTH MANDATORY HEALTH STANDARDS-COAL MINERS WHO HAVE EVIDENCE OF THE DEVELOPMENT OF PNEUMOCONIOSIS Sampling Procedures § 90.203 Certified person; maintenance and calibration. (a) Approved sampling devices shall be maintained and calibrated by a certified person. (b) To be certified, a...

  1. 27 CFR 555.203 - Types of magazines.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 3 2010-04-01 2010-04-01 false Types of magazines. 555... EXPLOSIVES, DEPARTMENT OF JUSTICE EXPLOSIVES COMMERCE IN EXPLOSIVES Storage § 555.203 Types of magazines. For purposes of this part, there are five types of magazines. These types, together with the classes of...

  2. 31 CFR 203.20 - Investment account requirements.

    Science.gov (United States)

    2010-07-01

    ... TREASURY TAX AND LOAN PROGRAM Investment Program and Collateral Security Requirements for TT&L Depositaries... investor depositary is willing to provide collateral in accordance with § 203.21(c)(1). The depositary must... results from a collateral revaluation as determined by the FRB. That portion of any PATAX or EFTPS tax...

  3. 24 CFR 203.370 - Pre-foreclosure sales.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Pre-foreclosure sales. 203.370...-foreclosure sales. (a) General. HUD will pay FHA insurance benefits to mortgagees in cases where, in accordance with all regulations and procedures applicable to pre-foreclosure sales, the mortgaged property is...

  4. 20 CFR 429.203 - When is a claim allowable?

    Science.gov (United States)

    2010-04-01

    ... of you, your agent, the members of your family, or your private employee (the standard to be applied... 429.203 Employees' Benefits SOCIAL SECURITY ADMINISTRATION ADMINISTRATIVE CLAIMS UNDER THE FEDERAL... travel under orders, including property in your custody or in the custody of a carrier, an agent or...

  5. 33 CFR 203.50 - Nonstructural alternatives to rehabilitation of flood control works.

    Science.gov (United States)

    2010-07-01

    ... rehabilitation of flood control works. 203.50 Section 203.50 Navigation and Navigable Waters CORPS OF ENGINEERS... DISASTER PROCEDURES Rehabilitation Assistance for Flood Control Works Damaged by Flood or Coastal Storm: The Corps Rehabilitation and Inspection Program § 203.50 Nonstructural alternatives to rehabilitation...

  6. 48 CFR 206.203 - Set-asides for small business concerns.

    Science.gov (United States)

    2010-10-01

    ... Competition After Exclusion of Sources 206.203 Set-asides for small business concerns. (b) Also no separate... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Set-asides for small business concerns. 206.203 Section 206.203 Federal Acquisition Regulations System DEFENSE ACQUISITION...

  7. 41 CFR 301-75.203 - May we provide the interviewee with a travel advance?

    Science.gov (United States)

    2010-07-01

    ... interviewee with a travel advance? 301-75.203 Section 301-75.203 Public Contracts and Property Management Federal Travel Regulation System TEMPORARY DUTY (TDY) TRAVEL ALLOWANCES AGENCY RESPONSIBILITIES 75-PRE-EMPLOYMENT INTERVIEW TRAVEL Obtaining Travel Services and Claiming Reimbursement § 301-75.203 May we provide...

  8. 41 CFR 301-71.203 - Who is responsible for the validity of the travel claim?

    Science.gov (United States)

    2010-07-01

    ... the validity of the travel claim? 301-71.203 Section 301-71.203 Public Contracts and Property Management Federal Travel Regulation System TEMPORARY DUTY (TDY) TRAVEL ALLOWANCES AGENCY RESPONSIBILITIES 71-AGENCY TRAVEL ACCOUNTABILITY REQUIREMENTS Travel Claims for Reimbursement § 301-71.203 Who is responsible...

  9. 10 CFR 835.203 - Combining internal and external equivalent doses.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Combining internal and external equivalent doses. 835.203 Section 835.203 Energy DEPARTMENT OF ENERGY OCCUPATIONAL RADIATION PROTECTION Standards for Internal and External Exposure § 835.203 Combining internal and external equivalent doses. (a) The total effective dose...

  10. 39 CFR 20.3 - Availability of the International Mail Manual.

    Science.gov (United States)

    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Availability of the International Mail Manual. 20.3 Section 20.3 Postal Service UNITED STATES POSTAL SERVICE INTERNATIONAL MAIL INTERNATIONAL POSTAL SERVICE § 20.3 Availability of the International Mail Manual. Copies of the International Mail Manual may...

  11. 48 CFR 1852.203-70 - Display of Inspector General Hotline Posters.

    Science.gov (United States)

    2010-10-01

    ... General Hotline Posters. 1852.203-70 Section 1852.203-70 Federal Acquisition Regulations System NATIONAL... Provisions and Clauses 1852.203-70 Display of Inspector General Hotline Posters. As prescribed in 1803.7001, insert the following clause: Display of Inspector General Hotline Posters (JUN 2001) (a) The Contractor...

  12. 48 CFR 1416.203 - Fixed-price contracts with economic price adjustment.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Fixed-price contracts with economic price adjustment. 1416.203 Section 1416.203 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Fixed-Price Contracts 1416.203...

  13. 48 CFR 16.203 - Fixed-price contracts with economic price adjustment.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Fixed-price contracts with economic price adjustment. 16.203 Section 16.203 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Fixed-Price Contracts 16.203 Fixed-price...

  14. 48 CFR 916.203 - Fixed-price contracts with economic price adjustments.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Fixed-price contracts with economic price adjustments. 916.203 Section 916.203 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Fixed-Price Contracts 916.203 Fixed...

  15. 48 CFR 216.203 - Fixed-price contracts with economic price adjustment.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Fixed-price contracts with economic price adjustment. 216.203 Section 216.203 Federal Acquisition Regulations System DEFENSE... CONTRACTS Fixed-Price Contracts 216.203 Fixed-price contracts with economic price adjustment. ...

  16. 48 CFR 1216.203 - Fixed-price contracts with economic price adjustment.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Fixed-price contracts with economic price adjustment. 1216.203 Section 1216.203 Federal Acquisition Regulations System DEPARTMENT OF TRANSPORTATION CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Fixed-Price Contracts 1216.203 Fixed...

  17. 48 CFR 5416.203 - Fixed-price contracts with economic price adjustment.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Fixed-price contracts with economic price adjustment. 5416.203 Section 5416.203 Federal Acquisition Regulations System DEFENSE LOGISTICS AGENCY, DEPARTMENT OF DEFENSE TYPES OF CONTRACTS Fixed Price Contracts 5416.203 Fixed-price...

  18. 48 CFR 416.203 - Fixed-price contracts with economic price adjustment.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Fixed-price contracts with economic price adjustment. 416.203 Section 416.203 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Fixed-Price Contracts 416.203 Fixed...

  19. 48 CFR 3016.203 - Fixed price contracts with economic price adjustments.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Fixed price contracts with economic price adjustments. 3016.203 Section 3016.203 Federal Acquisition Regulations System DEPARTMENT OF... TYPES OF CONTRACTS Fixed-Price Contracts 3016.203 Fixed price contracts with economic price adjustments. ...

  20. 48 CFR 616.203 - Fixed-Price contracts with economic price adjustment.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Fixed-Price contracts with economic price adjustment. 616.203 Section 616.203 Federal Acquisition Regulations System DEPARTMENT OF STATE CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Fixed-Price Contracts 616.203 Fixed...

  1. 48 CFR 1316.203 - Fixed-price contracts with economic price adjustment.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Fixed-price contracts with economic price adjustment. 1316.203 Section 1316.203 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Fixed-Price Contracts 1316.203 Fixed...

  2. 48 CFR 52.203-13 - Contractor Code of Business Ethics and Conduct.

    Science.gov (United States)

    2010-10-01

    ... business ethics and conduct. (C) Periodic reviews of company business practices, procedures, policies, and... Business Ethics and Conduct. 52.203-13 Section 52.203-13 Federal Acquisition Regulations System FEDERAL... Provisions and Clauses 52.203-13 Contractor Code of Business Ethics and Conduct. As prescribed in 3.1004(a...

  3. Target preparation by electroplating of enriched thallium-203 and its quality control

    International Nuclear Information System (INIS)

    Al-Rayyes, A. H.; Ailouti, Y.

    2009-02-01

    In this study, the optimum conditions of enriched thallium-203 electroplating on copper holders have been determined starting from different electroplating electrolytes. These conditions include current intensity and thallium concentration. The different solutions were: Newly prepared electrolyte using 203 Tl 2 O 3 , depleted solution used in a previous operations of electroplating, and recovery solutions, coming from Tl-203 separation from lead-201.(author)

  4. 48 CFR 14.203-2 - Dissemination of information concerning invitations for bids.

    Science.gov (United States)

    2010-10-01

    ... 14.203-2 Dissemination of information concerning invitations for bids. Procedures concerning display... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Dissemination of information concerning invitations for bids. 14.203-2 Section 14.203-2 Federal Acquisition Regulations System...

  5. 48 CFR 1652.203-70 - Misleading, deceptive, or unfair advertising.

    Science.gov (United States)

    2010-10-01

    ... unfair advertising. 1652.203-70 Section 1652.203-70 Federal Acquisition Regulations System OFFICE OF... CLAUSES Texts of FEHBP Clauses 1652.203-70 Misleading, deceptive, or unfair advertising. As prescribed in... Unfair Advertising (JAN 1991) (a) The Carrier agrees that any advertising material, including that...

  6. 48 CFR 852.203-71 - Display of Department of Veterans Affairs hotline poster.

    Science.gov (United States)

    2010-10-01

    ... Veterans Affairs hotline poster. 852.203-71 Section 852.203-71 Federal Acquisition Regulations System... Provisions and Clauses 852.203-71 Display of Department of Veterans Affairs hotline poster. As prescribed in 803.7001, insert the following clause: Display of Department of Veterans Affairs Hotline Poster (DEC...

  7. 48 CFR 1552.203-71 - Display of EPA Office of Inspector General Hotline poster.

    Science.gov (United States)

    2010-10-01

    ... Inspector General Hotline poster. 1552.203-71 Section 1552.203-71 Federal Acquisition Regulations System... Provisions and Clauses 1552.203-71 Display of EPA Office of Inspector General Hotline poster. As prescribed... all contract options. Display of EPA Office of Inspector General Hotline Poster (AUG 2000) (a) For EPA...

  8. 14 CFR 203.5 - Compliance as condition on operations in air transportation.

    Science.gov (United States)

    2010-01-01

    ... air transportation. 203.5 Section 203.5 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF... DEFENSES § 203.5 Compliance as condition on operations in air transportation. It shall be a condition on the authority of all direct U.S. and foreign carriers to operate in air transportation that they have...

  9. 30 CFR 203.82 - What is MMS's authority to collect this information?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What is MMS's authority to collect this information? 203.82 Section 203.82 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR... Reports § 203.82 What is MMS's authority to collect this information? The Office of Management and Budget...

  10. 30 CFR 203.70 - What information must I provide after MMS approves relief?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What information must I provide after MMS... information must I provide after MMS approves relief? You must submit reports to us as indicated in the following table. Sections 203.81, 203.90, and 203.91 describe what these reports must include. The MMS...

  11. 24 CFR 203.420 - Nature of Mutual Mortgage Insurance Fund.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Nature of Mutual Mortgage Insurance Fund. 203.420 Section 203.420 Housing and Urban Development Regulations Relating to Housing and Urban... and Distributive Shares § 203.420 Nature of Mutual Mortgage Insurance Fund. The Mutual Mortgage...

  12. 24 CFR 203.330 - Definition of delinquency and requirement for notice of delinquency to HUD.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Definition of delinquency and requirement for notice of delinquency to HUD. 203.330 Section 203.330 Housing and Urban Development... and Obligations Default Under Mortgage § 203.330 Definition of delinquency and requirement for notice...

  13. 24 CFR 203.466 - Definition of delinquency and requirement for notice of delinquency to HUD.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Definition of delinquency and requirement for notice of delinquency to HUD. 203.466 Section 203.466 Housing and Urban Development... and Obligations Rehabilitation Loans § 203.466 Definition of delinquency and requirement for notice of...

  14. 48 CFR 627.203 - Patent indemnification of Government by contractor.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Patent indemnification of Government by contractor. 627.203 Section 627.203 Federal Acquisition Regulations System DEPARTMENT OF STATE GENERAL CONTRACTING REQUIREMENTS PATENTS, DATA, AND COPYRIGHTS Patents 627.203 Patent indemnification of...

  15. 18 CFR 382.203 - Annual charges under the Interstate Commerce Act.

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Annual charges under the Interstate Commerce Act. 382.203 Section 382.203 Conservation of Power and Water Resources FEDERAL... § 382.203 Annual charges under the Interstate Commerce Act. (a) The adjusted costs of administration of...

  16. 24 CFR 4001.203 - Calculation of upfront and annual mortgage insurance premiums for Program mortgages.

    Science.gov (United States)

    2010-04-01

    ... mortgage insurance premiums for Program mortgages. 4001.203 Section 4001.203 Housing and Urban Development... HOMEOWNERS PROGRAM HOPE FOR HOMEOWNERS PROGRAM Rights and Obligations Under the Contract of Insurance § 4001.203 Calculation of upfront and annual mortgage insurance premiums for Program mortgages. (a...

  17. 24 CFR 203.260 - Amount of mortgage insurance premium (periodic MIP).

    Science.gov (United States)

    2010-04-01

    ... Mortgage Insurance Premiums-Periodic Payment § 203.260 Amount of mortgage insurance premium (periodic MIP... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Amount of mortgage insurance premium (periodic MIP). 203.260 Section 203.260 Housing and Urban Development Regulations Relating to...

  18. Determination of radiochemical purity of chlormerodrin-203 by electrophoretic method

    Energy Technology Data Exchange (ETDEWEB)

    Jovanovic, V; Zmbova, B; Bzenic, J [Institut za Nuklearne Nauke Boris Kidric, Belgrade (Yugoslavia)

    1977-01-01

    Experiments were performed with NaCl (Merck) as an electrolyte in the concentration range from 1.10/sup -5/ to 5.10/sup -2/N at pH 6.8 using low-voltage electrophoresis in a wet chamber (JEVIPLS-Ljujbljana) with a rectifier MA8901. 0.005 cm/sup 3/ of labelled neohydrin-203, at the concentration of 2-4 mg/cm/sup 3/ and with an activity of mCi/cm/sup 3/ was spotted on Whatman No 3, MM (30x2) chromatographic paper stripes. Correction for electroosmosis was performed with 5% H/sub 2/O/sub 2/, and 5% KI was used for the identification of peroxide mobility. Maximum separation of /sup 203/Hg adn and hydroxychlormerodrin was achieved at 0.05 M NaCl at the potential gradient of 8 V/cm for 2 hrs.

  19. 27 CFR 9.203 - Saddle Rock-Malibu.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Saddle Rock-Malibu. 9.203... Saddle Rock-Malibu. (a) Name. The name of the viticultural area described in this section is “Saddle Rock-Malibu”. For purposes of part 4 of this chapter, “Saddle Rock-Malibu” is a term of viticultural...

  20. Nuclear resonance fluorescence of {sup 203,205}Tl

    Energy Technology Data Exchange (ETDEWEB)

    Pfeifer, Fabian; Fritzsche, Matthias; Pietralla, Norbert; Savran, Deniz; Weller, Henry; Zweidinger, Markus [Institut fuer Kernphysik, Technische Universitaet, Darmstadt (Germany); Rusev, Gencho; Tonchev, Anton P.; Tornow, Werner [Triangle Universities Nuclear Laboratory, Duke University, Durham (United States); Zilges, Andreas [Institut fuer Kernphysik, Universitaet Koeln (Germany)

    2009-07-01

    In order to investigate the dipole strength distribution in Thalium isotopes we have studied Nuclear Resonance Fluorescence of a sample composed of natural Thallium (consisting of 30% {sup 203}Tl and 70% {sup 205}Tl). Unpolarized bremsstrahlung with photo energies up to 7.5 MeV was used at the High Intensity Photon Setup (HIPS) at S-DALINAC at the IKP Darmstadt. 24 fluorescent {gamma}-ray transitions were observed, 19 of them for the first time. For the assignment of the polarity of two prominent {gamma}-ray transitions, one at 4.7 MeV and one at 4.9 MeV, the polarized photon beam of the High Intensity {gamma}-ray Source (HI{gamma}S) at Duke University was used. The experiment at HI{gamma}S revealed the existence of a photo-excited state of {sup 205}Tl at an excitation energy of 4.971 MeV that exhibits a transition to the first excited state at 203 keV.

  1. 28 CFR 301.203 - Payment of lost-time wages.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Payment of lost-time wages. 301.203... ACCIDENT COMPENSATION Lost-Time Wages § 301.203 Payment of lost-time wages. (a) An inmate worker may receive lost-time wages for the number of regular work hours absent from work due to injury sustained in...

  2. 40 CFR 267.203 - What special requirements must I meet for incompatible wastes?

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false What special requirements must I meet for incompatible wastes? 267.203 Section 267.203 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE...

  3. 10 CFR 490.203 - Light Duty Alternative Fueled Vehicle Plan.

    Science.gov (United States)

    2010-01-01

    ... Efficiency and Renewable Energy, EE-33, 1000 Independence Ave., SW., Washington, DC 20585, or to such other... 10 Energy 3 2010-01-01 2010-01-01 false Light Duty Alternative Fueled Vehicle Plan. 490.203 Section 490.203 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ALTERNATIVE FUEL TRANSPORTATION PROGRAM...

  4. 40 CFR 266.203 - Standards applicable to the transportation of solid waste military munitions.

    Science.gov (United States)

    2010-07-01

    ... transportation of solid waste military munitions. 266.203 Section 266.203 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR THE MANAGEMENT OF SPECIFIC HAZARDOUS... applicable to the transportation of solid waste military munitions. (a) Criteria for hazardous waste...

  5. 20 CFR 726.203 - Federal Coal Mine Health and Safety Act endorsement.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Federal Coal Mine Health and Safety Act endorsement. 726.203 Section 726.203 Employees' Benefits EMPLOYMENT STANDARDS ADMINISTRATION, DEPARTMENT OF LABOR FEDERAL COAL MINE HEALTH AND SAFETY ACT OF 1969, AS AMENDED BLACK LUNG BENEFITS; REQUIREMENTS FOR...

  6. 48 CFR 2152.203-70 - Misleading, deceptive, or unfair advertising.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Misleading, deceptive, or unfair advertising. 2152.203-70 Section 2152.203-70 Federal Acquisition Regulations System OFFICE OF PERSONNEL MANAGEMENT, FEDERAL EMPLOYEES GROUP LIFE INSURANCE FEDERAL ACQUISITION REGULATION CLAUSES AND FORMS PRECONTRACT PROVISIONS AND CONTRACT...

  7. 24 CFR 203.356 - Notice of foreclosure and pre-foreclosure sale; reasonable diligence requirements.

    Science.gov (United States)

    2010-04-01

    ...-foreclosure sale; reasonable diligence requirements. 203.356 Section 203.356 Housing and Urban Development... diligence requirements. (a) Notice of foreclosure and pre-foreclosure sale. The mortgagee must give notice... pre-foreclosure sale procedure. (b) Reasonable diligence. The mortgagee must exercise reasonable...

  8. 9 CFR 93.203 - Ports designated for the importation of poultry.

    Science.gov (United States)

    2010-01-01

    ... of poultry. 93.203 Section 93.203 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS IMPORTATION OF CERTAIN ANIMALS, BIRDS, FISH, AND POULTRY, AND CERTAIN ANIMAL, BIRD, AND POULTRY...

  9. 17 CFR 275.203-2 - Withdrawal from investment adviser registration.

    Science.gov (United States)

    2010-04-01

    ... application). (b) Electronic filing. Once you have filed your Form ADV (17 CFR 279.1) (or any amendments to... file must be filed with the IARD, unless you have received a hardship exemption under § 275.203-3. (c... EXCHANGE COMMISSION (CONTINUED) RULES AND REGULATIONS, INVESTMENT ADVISERS ACT OF 1940 § 275.203-2...

  10. 7 CFR 205.203 - Soil fertility and crop nutrient management practice standard.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Soil fertility and crop nutrient management practice standard. 205.203 Section 205.203 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT...

  11. Mercury material-balance in industrial electrolytic cells, by using radioactive mercury (203Hg)

    International Nuclear Information System (INIS)

    Caras, I.; Pasi, M.

    1976-01-01

    A material-balance test for industrial mercury electrolytic cells is described. The test uses the radioactive dilution technique with 203 Hg. The preparation of the 203 Hg from irradiated mercuric oxide is also described. The accuracy of the test is shown to be +-1% for each cell. (author)

  12. 5 CFR 2638.203 - Duties of the designated agency ethics official.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Duties of the designated agency ethics official. 2638.203 Section 2638.203 Administrative Personnel OFFICE OF GOVERNMENT ETHICS GOVERNMENT ETHICS OFFICE OF GOVERNMENT ETHICS AND EXECUTIVE AGENCY ETHICS PROGRAM RESPONSIBILITIES Designated Agency Ethics...

  13. 38 CFR 1.203 - Information to be reported to VA Police.

    Science.gov (United States)

    2010-07-01

    ... reported to VA Police. 1.203 Section 1.203 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS... be reported to VA Police. Information about actual or possible violations of criminal laws related to... occurs on VA premises, will be reported by VA management officials to the VA police component with...

  14. 20 CFR 703.203 - Application for security deposit determination; information to be submitted; other requirements.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Application for security deposit determination; information to be submitted; other requirements. 703.203 Section 703.203 Employees' Benefits... each insurance rating service designated by the Branch and posted on the Internet at http://www.dol.gov...

  15. 41 CFR 105-54.203-3 - Submission to Library of Congress.

    Science.gov (United States)

    2010-07-01

    ... Congress. 105-54.203-3 Section 105-54.203-3 Public Contracts and Property Management Federal Property... Submission to Library of Congress. The GSA Committee Management Officer furnishes a copy of each charter to the Library of Congress when or shortly after copies are filed with the requisite committees of the...

  16. 21 CFR 203.32 - Drug sample storage and handling requirements.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 4 2010-04-01 2010-04-01 false Drug sample storage and handling requirements. 203.32 Section 203.32 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN... contamination, deterioration, and adulteration. (b) Compliance with compendial and labeling requirements...

  17. 30 CFR 203.66 - What happens if MMS does not act in the time allowed?

    Science.gov (United States)

    2010-07-01

    ... Relief for Pre-Act Deep Water Leases and for Development and Expansion Projects § 203.66 What happens if MMS does not act in the time allowed? If we do not act within the timeframes established under § 203... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What happens if MMS does not act in the time...

  18. 24 CFR 203.256 - Insurance of open-end advance.

    Science.gov (United States)

    2010-04-01

    ... Insurance § 203.256 Insurance of open-end advance. Insurance on an open-end advance will be evidenced by delivery of a certificate stating the amount of the advance, the date of insurance, and the regulations... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Insurance of open-end advance. 203...

  19. 30 CFR 77.203 - Use of material or equipment overhead; safeguards.

    Science.gov (United States)

    2010-07-01

    ...; safeguards. 77.203 Section 77.203 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR COAL MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS, SURFACE COAL MINES AND SURFACE WORK AREAS...; safeguards. Where overhead repairs are being made at surface installations and equipment or material is taken...

  20. 48 CFR 1631.203-71 - Business unit General and Administrative (G&A) expenses.

    Science.gov (United States)

    2010-10-01

    ... Business unit General and Administrative (G&A) expenses. G&A expenses shall be allocated to final cost... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Business unit General and Administrative (G&A) expenses. 1631.203-71 Section 1631.203-71 Federal Acquisition Regulations System OFFICE OF...

  1. 48 CFR 3003.203 - Reporting suspected violations of the Gratuities clause.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Reporting suspected violations of the Gratuities clause. 3003.203 Section 3003.203 Federal Acquisition Regulations System DEPARTMENT OF HOMELAND SECURITY, HOMELAND SECURITY ACQUISITION REGULATION (HSAR) GENERAL IMPROPER BUSINESS...

  2. 40 CFR 141.203 - Tier 2 Public Notice-Form, manner, and frequency of notice.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Tier 2 Public Notice-Form, manner, and frequency of notice. 141.203 Section 141.203 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY...., house renters, apartment dwellers, university students, nursing home patients, prison inmates, etc...

  3. 27 CFR 19.203 - Alternation of distilled spirits plant and bonded wine cellar premises.

    Science.gov (United States)

    2010-04-01

    ... spirits plant and bonded wine cellar premises. 19.203 Section 19.203 Alcohol, Tobacco Products and... spirits plant and bonded wine cellar premises. (a) General. A proprietor of a distilled spirits plant operating a contiguous bonded wine cellar desiring to alternate the use of each premises by extension and...

  4. Astatine-211 labeling. A study towards automatic production of astatinated antibodies

    International Nuclear Information System (INIS)

    Emma Aneheim; Per Albertsson; Sture Lindegren; Holger Jensen

    2015-01-01

    Targeted alpha therapy is especially interesting for therapy of microscopic cancer tumors due to short path length and high linear energy transfer of the alpha particles. One of the most promising nuclides for targeted alpha therapy is 211 At. To facilitate larger clinical studies using 211 At, the current manual synthesis of radiolabeled antibodies would benefit from being transferred into an automated method. In this work, successful modifications of the manual synthesis have been performed in order to adapt it to automation. The automatic synthesis has also been tested using the modified synthesis method. (author)

  5. Glaucoma associated with iridocorneal endothelial syndrome in 203 Indian subjects.

    Directory of Open Access Journals (Sweden)

    Premanand Chandran

    Full Text Available To report the demographic profile, clinical features, and prevalence of glaucoma and its management in patients with Iridocorneal endothelial (ICE syndrome.Retrospective review of 203 consecutive subjects with ICE syndrome at a tertiary eye care centre between January 1988 and June 2013.ICE syndrome was present in 223 eyes of 203 subjects, 124 (61% were female and 79 (39% were male. The median age at presentation was 43 years (1st (Q1 and 3rd (Q3 quartile; 34, 51 years. ICE syndrome was unilateral in 183 (90% subjects, and bilateral in 20 (10% subjects. The most common clinical variant was progressive iris atrophy (PIA, 115; 52% eyes, followed by Chandler syndrome (CS, 87; 39% eyes and Cogan-Reese syndrome (CRS, 21; 9% eyes. Glaucoma was found in 156 eyes (70% at presentation and the median (Q1, Q3 intraocular pressure in eyes with glaucoma was 24 (16, 38 mm Hg. Seven eyes developed glaucoma during the follow-up period, increasing the percentage of eyes with glaucoma to 73%. Intraocular pressure was managed medically in 81 eyes (50% and the other 82 eyes (50% required surgical intervention. Corneal edema was present in 124 eyes (56% of which, 32 eyes (14% required keratoplasty.In our study on ICE syndrome in Indian population, the presentation was predominantly uniocular and more common in middle aged women. Progressive iris atrophy was the most common clinical variant. ICE syndrome was associated with glaucoma in over 70% of the eyes and half of the eyes had corneal edema.

  6. 30 CFR 203.64 - How many applications may I file on a field or a development project?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false How many applications may I file on a field or a development project? 203.64 Section 203.64 Mineral Resources MINERALS MANAGEMENT SERVICE... § 203.64 How many applications may I file on a field or a development project? You may file one complete...

  7. 49 CFR 40.203 - What problems cause a drug test to be cancelled unless they are corrected?

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false What problems cause a drug test to be cancelled unless they are corrected? 40.203 Section 40.203 Transportation Office of the Secretary of Transportation PROCEDURES FOR TRANSPORTATION WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Problems in Drug Tests § 40.203...

  8. 14 CFR 203.4 - Montreal Agreement as part of airline-passenger contract and conditions of carriage.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Montreal Agreement as part of airline-passenger contract and conditions of carriage. 203.4 Section 203.4 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS WAIVER OF WARSAW CONVENTION LIABILITY LIMITS AND DEFENSES § 203.4...

  9. 40 CFR 26.203 - Prohibition of research conducted or supported by EPA involving intentional exposure of any human...

    Science.gov (United States)

    2010-07-01

    ... her fetus), a nursing woman, or child. 26.203 Section 26.203 Protection of Environment ENVIRONMENTAL... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Prohibition of research conducted or... Involving Intentional Exposure of Human Subjects who are Children or Pregnant or Nursing Women § 26.203...

  10. MicroRNA-203 Modulates the Radiation Sensitivity of Human Malignant Glioma Cells

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Ji Hyun [Department of Radiation Oncology, Graduate School of Medicine, Seoul National University, Seoul (Korea, Republic of); Hwang, Yeo Hyun; Lee, David J.; Kim, Dan Hyo; Park, Ji Min [Medical Science Research Institute, Seoul National University Bundang Hospital, Kyeonggido (Korea, Republic of); Wu, Hong-Gyun [Department of Radiation Oncology, Graduate School of Medicine, Seoul National University, Seoul (Korea, Republic of); Kim, In Ah, E-mail: inah228@snu.ac.kr [Department of Radiation Oncology, Graduate School of Medicine, Seoul National University, Seoul (Korea, Republic of); Medical Science Research Institute, Seoul National University Bundang Hospital, Kyeonggido (Korea, Republic of); Cancer Research Institute, Seoul National University, Seoul (Korea, Republic of)

    2016-02-01

    Purpose: We investigated whether miR-203 could modulate the radiation sensitivity of glioblastoma (GBM) cells and which target gene(s) could be involved. Methods and Materials: Three human malignant glioma (MG) cell lines and normal human astrocytes were transfected with control microRNA, pre-miR-203, or antisense miR-203. Real-time PCR (RT-PCR), clonogenic assays, immunofluorescence, and invasion/migration assays were performed. To predict the target(s), bioinformatics analyses using microRNA target databases were performed. Results: Overexpression of miR-203 increased the radiation sensitivity of all 3 human MG cell lines and prolonged radiation-induced γ-H2AX foci formation. Bioinformatics analyses suggested that miR-203 could be involved in post-transcriptional control of DNA repair, PI3K/AKT, SRC, and JAK/STAT3 and the vascular signaling pathway. Western blot analysis validated the fact that miR-203 downregulated ATM, RAD51, SRC, PLD2, PI3K-AKT, JAK-STAT3, VEGF, HIF-1α, and MMP2. Overexpression of miR-203 inhibited invasion and migration potentials, downregulated SLUG and Vimentin, and upregulated Claudin-1 and ZO1. Conclusions: These data demonstrate that miR-203 potentially controls DNA damage repair via the PI3K/AKT and JAK/STAT3 pathways and may collectively contribute to the modulation of radiation sensitivity in MG cells by inhibiting DNA damage repair, prosurvival signaling, and epithelium-mesenchyme transition. Taken together, these findings demonstrate that miR-203 could be a target for overcoming the radiation resistance of GBM.

  11. MicroRNA-203 Modulates the Radiation Sensitivity of Human Malignant Glioma Cells

    International Nuclear Information System (INIS)

    Chang, Ji Hyun; Hwang, Yeo Hyun; Lee, David J.; Kim, Dan Hyo; Park, Ji Min; Wu, Hong-Gyun; Kim, In Ah

    2016-01-01

    Purpose: We investigated whether miR-203 could modulate the radiation sensitivity of glioblastoma (GBM) cells and which target gene(s) could be involved. Methods and Materials: Three human malignant glioma (MG) cell lines and normal human astrocytes were transfected with control microRNA, pre-miR-203, or antisense miR-203. Real-time PCR (RT-PCR), clonogenic assays, immunofluorescence, and invasion/migration assays were performed. To predict the target(s), bioinformatics analyses using microRNA target databases were performed. Results: Overexpression of miR-203 increased the radiation sensitivity of all 3 human MG cell lines and prolonged radiation-induced γ-H2AX foci formation. Bioinformatics analyses suggested that miR-203 could be involved in post-transcriptional control of DNA repair, PI3K/AKT, SRC, and JAK/STAT3 and the vascular signaling pathway. Western blot analysis validated the fact that miR-203 downregulated ATM, RAD51, SRC, PLD2, PI3K-AKT, JAK-STAT3, VEGF, HIF-1α, and MMP2. Overexpression of miR-203 inhibited invasion and migration potentials, downregulated SLUG and Vimentin, and upregulated Claudin-1 and ZO1. Conclusions: These data demonstrate that miR-203 potentially controls DNA damage repair via the PI3K/AKT and JAK/STAT3 pathways and may collectively contribute to the modulation of radiation sensitivity in MG cells by inhibiting DNA damage repair, prosurvival signaling, and epithelium-mesenchyme transition. Taken together, these findings demonstrate that miR-203 could be a target for overcoming the radiation resistance of GBM.

  12. Conventional superconductivity at 203 K at high pressures

    Energy Technology Data Exchange (ETDEWEB)

    Drozdov, Alexander; Eremets, Mikhail; Troyan, Ivan [Max-Planck-Institut fuer Chemie, Hahn-Meitner-Weg 1, 55128 Mainz (Germany); Ksenofontov, Vadim; Shylin, Sergii [Institut fuer Anorganische Chemie und Analytische Chemie, Johannes Gutenberg-Universitet Mainz, Staudingerweg 9, 55099 Mainz (Germany)

    2016-07-01

    A search for high, room temperature conventional superconductivity is promising as the Bardeen-Cooper-Schrieffer (BCS) theory in the Eliashberg formulation puts no apparent limits on T{sub c}. Materials with light elements are especially favorable as they provide high frequencies in the phonon spectrum. However only a moderately high T{sub c} = 39 K has been found in this search in MgB{sub 2}. We systematically studied metallic hydrogen and covalent hydrogen dominant compounds and found the record T{sub c} of 203 K at pressure 140 GPa in sulfur hydride. We proved occurrence of superconductivity by the sharp drop of the resistivity to zero; the decrease of T{sub c} with magnetic field; the pronounce isotope shift of T{sub c} in D{sub 2}S which evidences of a major role of phonons in the superconductivity; and the magnetic susceptibility measurements. The X-ray diffraction data confirmed that the superconductive phase has the predicted bcc structure. This phase can be considered as an atomic hydrogen superconductor stabilized by sulfur.

  13. A norming study and library of 203 dance movements.

    Science.gov (United States)

    Christensen, Julia F; Nadal, Marcos; Cela-Conde, Camilo José

    2014-01-01

    Dance stimuli have been used in experimental studies of (i) how movement is processed in the brain; (ii) how affect is perceived from bodily movement; and (iii) how dance can be a source of aesthetic experience. However, stimulus materials across--and even within--these three domains of research have varied considerably. Thus, integrative conclusions remain elusive. Moreover, concerns have been raised that the movements selected for such stimuli are qualitatively too different from the actual art form dance, potentially introducing noise in the data. We propose a library of dance stimuli which responds to the stimuli requirements and design criteria of these three areas of research, while at the same time respecting a dance art-historical perspective, offering greater ecological validity as compared with previous dance stimulus sets. The stimuli are 5-6 s long video clips, selected from genuine ballet performances. Following a number of coding experiments, the resulting stimulus library comprises 203 ballet dance stimuli coded in (i) 25 qualitative and quantitative movement variables; (ii) affective valence and arousal; and (iii) the aesthetic qualities beauty, liking, and interest. An Excel spreadsheet with these data points accompanies this manuscript, and the stimuli can be obtained from the authors upon request.

  14. REFORMING THE NE BIS IN IDEM CLAUSE OF ARTICLE 20(3)

    African Journals Online (AJOL)

    Fr. Ikenga

    deal with all perpetrators of the crimes that come under its jurisdiction wherever such crimes are ... KAYITANA: Complementarity and Completed Trials: Reforming the Ne Bis in Idem Clause of Article 20(3) of ..... exchange for lenient sentences.

  15. Differential determination of 203Hg and 14C or 35S in double labelled biological samples

    International Nuclear Information System (INIS)

    Bem, E.M.; Bem, H.; Reimschussel, W.

    1979-01-01

    The differential determination of 203 Hg and 14 C or 35 S in double labelled biological samples is presented. The biological samples were mineralized with 70% HClO 4 and 30% H 2 O 2 in glass vials, MILLI-6. The γ-activity of 203 Hg was measured on a well scintillation counter. The total activity, due to 203 Hg and 14 C or 35 S, was measured by the liquid scintillation technique after addition of Aquasol into the same vials. The method of external standard channel ratio was used for standardization. Very good recoveries were obtained: 100+-0.7% for 203 Hg and 94.6-101.0% for 14 C and 35 S. This method could be used for other β, γ and β-active nuclides with similar β-spectra. (author)

  16. 31 CFR 203.6 - Obligations of TT&L depositaries.

    Science.gov (United States)

    2010-07-01

    ... TREASURY TAX AND LOAN PROGRAM General Information § 203.6 Obligations of TT&L depositaries. A TT&L depositary must: (a) Administer a TIP main account balance, SDI account balance, or TIO account balance, as...

  17. 31 CFR 203.8 - Application of part and procedural instructions.

    Science.gov (United States)

    2010-07-01

    ... AND THE TREASURY TAX AND LOAN PROGRAM General Information § 203.8 Application of part and procedural... account balance, SDI account balance, or a TIO account balance under this part. By accepting or...

  18. 31 CFR 203.5 - Designation of financial institutions as TT&L depositaries.

    Science.gov (United States)

    2010-07-01

    ... PAYMENT OF FEDERAL TAXES AND THE TREASURY TAX AND LOAN PROGRAM General Information § 203.5 Designation of... authorized to maintain a TT&L account, TIP main account balance, SDI account balance, or TIO account balance...

  19. Sevoflurane suppresses proliferation by upregulating microRNA-203 in breast cancer cells.

    Science.gov (United States)

    Liu, Jiaying; Yang, Longqiu; Guo, Xia; Jin, Guangli; Wang, Qimin; Lv, Dongdong; Liu, Junli; Chen, Qiu; Song, Qiong; Li, Baolin

    2018-05-03

    Rapid proliferation is one of the critical characteristics of breast cancer. However, the underlying regulatory mechanism of breast cancer cell proliferation is largely unclear. The present study indicated that sevoflurane, one of inhalational anesthetics, could significantly suppress breast cancer cell proliferation by arresting cell cycle at G1 phase. Notably, the rescue experiment indicated that miR-203 was upregulated by sevoflurane and mediated the function of sevoflurane on suppressing the breast cancer cell proliferation. The present study indicated the function of the sevoflurane/miR-203 signaling pathway on regulating breast cancer cell proliferation. These results provide mechanistic insight into how the sevoflurane/miR-203 signaling pathway supresses proliferation of breast cancer cells, suggesting the sevoflurane/miR-203 pathway may be a potential target in the treatment of breast cancer.

  20. 5 CFR 591.203 - Why does the Government pay post differentials?

    Science.gov (United States)

    2010-01-01

    ... REGULATIONS ALLOWANCES AND DIFFERENTIALS Cost-of-Living Allowance and Post Differential-Nonforeign Areas Cost-Of-Living Allowances and Post Differentials § 591.203 Why does the Government pay post differentials...

  1. Hydrogen embrittlement of ASTM A 203 D nuclear structural steel

    International Nuclear Information System (INIS)

    Chakravartty, J.K.; Prasad, G.E.; Sinha, T.K.; Asundi, M.K.

    1986-01-01

    The influence of hydrogen on the mechanical properties of ASTM A 203 D nuclear structural steel has been studied by tension, bend and delayed-failure tests at room temperature. While the tension tests of hydrogen charged unnotched specimens reveal no change in ultimate strength and ductility, the effect of hydrogen is manifested in notched specimens (tensile and bend) as a decrease in ultimate strength (maximum load in bend test) and ductility; the effect increases with increasing hydrogen content. It is observed that for a given hydrogen concentration, the decrease in bend ductility is remarkably large compared to that in tensile ductility. Hydrogen charging does not cause any delayed-failure upto 200 h under an applied tensile stress, 0.85 times the notch tensile strength. However delayed failure occurs in hydrogen charged bend samples in less than 10 h under an applied bending load of about 0.80 times of the uncharged maximum load. Fractographs of hydrogen charged unnotched specimens show ductile dimple fracture, while those of notched tension and bend specimens under hydrogen-charged conditions show a mixture of ductile dimple and quasi-cleavage cracking. The proportion of quasi-cleavage cracking increases with increasing hydrogen content and this fracture mode is more predominant in bend specimens. The changes in tensile properties and fracture modes can reasonably be explained by existing theories of hydrogen embrittlement. An attempt is made to explain the significant difference in the embrittlement susceptibility of bend and tensile specimens in the light of difference in triaxiality and plastic zone size near the notch tip. (orig.)

  2. MicroRNA-203-mediated posttranscriptional deregulation of CPEB4 contributes to colorectal cancer progression

    International Nuclear Information System (INIS)

    Zhong, Xiaohua; Xiao, Yipin; Chen, Chao; Wei, Xiuwen; Hu, Chen; Ling, Xukun; Liu, Xinbin

    2015-01-01

    Elevated cytoplasmic polyadenylation element-binding 4 (CPEB4) is aberrantly expressed in several malignant cancers. However, its expression pattern, clinical significance, and biological function in colorectal cancer are still unknown. In this study, we demonstrated that CPEB4 is abundantly overexpressed in colorectal cancers and has the potential to be used for predicting clinical outcomes of colorectal cancer patients. We suppressed CPEB4 expression by small interfering RNA (siRNA) in SW480 and LOVO cells to clarify the role of CPEB4 on the cell apoptosis and proliferation in vitro. Further study revealed that knockdown of CPEB4 decreased the expression of anti-apoptotic protein B-cell lymphoma-extra large (Bcl-XL), but enhanced the expression of B-cell lymphoma-2-associated X (Bax). In addition, we indicated that CPEB4 is a novel target of miR-203, a tumor suppressive microRNA. Notably, restoration of CPEB4 in SW480 cells inhibited miR-203-induced apoptosis signaling pathway, which in turn enhanced cell proliferation and suppressed cell apoptosis. Taken together, our findings imply that posttranscriptional deregulation of CPEB4 contributes to the inhibited cell proliferation and the enhanced cell apoptosis in colorectal cancer, and directly targeting CPEB4 by miR-203 might be a novel strategy in colorectal cancer treatment. - Highlights: • CPEB4 is aberrantly expressed in human colorectal cancers. • Knockdown of CPEB4 inhibited colorectal cancer cell proliferation and enhanced apoptosis. • CPEB4 is a direct target of miR-203 and inversely correlates with miR-203 expression. • miR-203 inhibited cell growth and enhanced cell apoptosis in CPEB4 dependent manner. • miR-203 is an upstream regulator of the CPEB4-induced apoptosis pathway.

  3. MicroRNA-203-mediated posttranscriptional deregulation of CPEB4 contributes to colorectal cancer progression

    Energy Technology Data Exchange (ETDEWEB)

    Zhong, Xiaohua; Xiao, Yipin; Chen, Chao, E-mail: chenchaopw@126.com; Wei, Xiuwen; Hu, Chen; Ling, Xukun; Liu, Xinbin

    2015-10-16

    Elevated cytoplasmic polyadenylation element-binding 4 (CPEB4) is aberrantly expressed in several malignant cancers. However, its expression pattern, clinical significance, and biological function in colorectal cancer are still unknown. In this study, we demonstrated that CPEB4 is abundantly overexpressed in colorectal cancers and has the potential to be used for predicting clinical outcomes of colorectal cancer patients. We suppressed CPEB4 expression by small interfering RNA (siRNA) in SW480 and LOVO cells to clarify the role of CPEB4 on the cell apoptosis and proliferation in vitro. Further study revealed that knockdown of CPEB4 decreased the expression of anti-apoptotic protein B-cell lymphoma-extra large (Bcl-XL), but enhanced the expression of B-cell lymphoma-2-associated X (Bax). In addition, we indicated that CPEB4 is a novel target of miR-203, a tumor suppressive microRNA. Notably, restoration of CPEB4 in SW480 cells inhibited miR-203-induced apoptosis signaling pathway, which in turn enhanced cell proliferation and suppressed cell apoptosis. Taken together, our findings imply that posttranscriptional deregulation of CPEB4 contributes to the inhibited cell proliferation and the enhanced cell apoptosis in colorectal cancer, and directly targeting CPEB4 by miR-203 might be a novel strategy in colorectal cancer treatment. - Highlights: • CPEB4 is aberrantly expressed in human colorectal cancers. • Knockdown of CPEB4 inhibited colorectal cancer cell proliferation and enhanced apoptosis. • CPEB4 is a direct target of miR-203 and inversely correlates with miR-203 expression. • miR-203 inhibited cell growth and enhanced cell apoptosis in CPEB4 dependent manner. • miR-203 is an upstream regulator of the CPEB4-induced apoptosis pathway.

  4. MicroRNA203a suppresses glioma tumorigenesis through an ATM-dependent interferon response pathway.

    Science.gov (United States)

    Yang, Chuan He; Wang, Yinan; Sims, Michelle; Cai, Chun; He, Ping; Häcker, Hans; Yue, Junming; Cheng, Jinjun; Boop, Frederick A; Pfeffer, Lawrence M

    2017-12-22

    Glioblastoma (GBM) is a deadly and incurable brain tumor. Although microRNAs (miRNAs) play critical roles in regulating the cancer cell phenotype, the underlying mechanisms of how they regulate tumorigenesis are incompletely understood. We previously showed that miR-203a is expressed at relatively low levels in GBM patients, and ectopic miR-203a expression in GBM cell lines inhibited cell proliferation and migration, increased sensitivity to apoptosis induced by interferon (IFN) or temozolomide in vitro , and inhibited GBM tumorigenesis in vivo . Here we show that ectopic expression of miR-203a in GBM cell lines promotes the IFN response pathway as evidenced by increased IFN production and IFN-stimulated gene (ISG) expression, and high basal tyrosine phosphorylation of multiple STAT proteins. Importantly, we identified that miR-203a directly suppressed the protein levels of ataxia-telangiectasia mutated (ATM) kinase that negatively regulates IFN production. We found that high ATM expression in GBM correlates with poor patient survival and that ATM expression is inversely correlated with miR-203a expression. Knockout of ATM expression and inhibition of ATM function in GBM cell lines inhibited cell proliferation and migration, increased sensitivity to apoptosis induced by therapeutic agents in vitro , and markedly suppressed GBM tumor growth and promoted animal survival. In contrast, restoring ATM levels in GBM cells ectopically expressing miR-203a increased tumorigenicity and decreased animal survival. Our study suggests that low miR-203a expression in GBM suppresses the interferon response through an ATM-dependent pathway.

  5. miR-203 inhibits melanoma invasive and proliferative abilities by targeting the polycomb group gene BMI1

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Xiao [Department of Dermatology and Venereal Disease, Xuanwu Hospital, Capital Medical University, Beijing 100053 (China); Sun, Yong [Department of Burn and Plastic Surgery, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an 223300 (China); Han, Siqi [Department of Medical Oncology, Jinling Hospital, Nanjing 210002 (China); Zhu, Wei [Department of Dermatology and Venereal Disease, Xuanwu Hospital, Capital Medical University, Beijing 100053 (China); Zhang, Haiping, E-mail: zhanghaiping_2000@163.com [Department of Dermatology and Venereal Disease, Xuanwu Hospital, Capital Medical University, Beijing 100053 (China); Lian, Shi, E-mail: lianshi_2020@163.com [Department of Dermatology and Venereal Disease, Capital Medical University, Beijing 100069 (China)

    2015-01-02

    Highlights: • First reported deregulation of miR-203 and up-regulation of BMI1 in metastatic melanoma. • miR-203 decreased BMI1 expression by directly binding to 3′UTR. • Further found miR-203 overexpression suppressed cell invasion and stemness. • Re-expression of BMI1 rescued miR-203-mediated suppression. • miR-203-BMI1 axis may be potential therapeutic targets of melanoma metastasis. - Abstract: Metastasis is the major problem in malignant melanoma, posing a therapeutic challenge to clinicians. The investigation of the underlying mechanism driving this progress remains a large unmet need. In this study, we revealed a miR-203-BMI1 axis that regulated melanoma metastasis. We found significantly deregulation of miR-203 and up-regulation of BMI1 in melanoma, particularly in metastatic melanoma. An inverse correlation between the levels of miR-203 and BMI1 was further observed in melanoma tissues and cell lines. We also identified BMI1 as a downstream target gene of miR-203, which bound to the 3′UTR of BMI1. Overexpression of miR-203 was associated with decreased BMI1 expression and impaired cell invasion and tumor sphere formation activities. Re-expression of BMI1 markedly rescued miR-203-mediated suppression of these events. Taken together, our results demonstrated that miR-203 regulated melanoma invasive and proliferative abilities in part by targeting BMI1, providing new insights into potential mechanisms of melanoma metastasis.

  6. miR-203 inhibits cell proliferation and promotes cisplatin induced cell death in tongue squamous cancer

    Energy Technology Data Exchange (ETDEWEB)

    Lin, Jiong; Lin, Yao [Guangdong Provincial Key Laboratory of Stomatology, Department of Orthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, 510055 (China); Fan, Li [Department of Pharmaceutical Analysis, School of Pharmacy, The Fourth Military Medical University, Xi' an, Shaanxi, 710032 (China); Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, 510055 (China); Kuang, Wei [Department of Stomatology, Guangzhou General Hospital of Guangzhou Military Command, 111 Liuhua Road, Guangzhou, 510010 (China); Zheng, Liwei [State Key Laboratory of Oral Diseases, Sichuan University, Wuhou District, Chengdu, 610041 (China); Wu, Jiahua [Guangdong Provincial Key Laboratory of Stomatology, Department of Orthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, 510055 (China); Shang, Peng [Patient-specific Orthopedic Technology Research Center in GuangDong Research Centre for Neural Engineering, 1068 Xueyuan Boulevard, University Town of Shenzhen, Xili, Nanshan, Shenzhen, 518055 (China); Wang, Qiaofeng [Department of Pharmaceutical Chemistry, School of Pharmacy, The Fourth Military Medical University, Xi' an, Shanxi, 710032 (China); Tan, Jiali, E-mail: jasminenov@163.com [Guangdong Provincial Key Laboratory of Stomatology, Department of Orthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, 510055 (China)

    2016-04-29

    Oral squamous cell carcinoma (OSCC) is one of the most common types of the head and neck cancer. Chemo resistance of OSCC has been identified as a substantial therapeutic hurdle. In this study, we analyzed the role of miR-203 in the OSCC and its effects on cisplatin-induced cell death in an OSCC cell line, Tca8113. There was a significant decrease of miR-203 expression in OSCC samples, compared with the adjacent normal, non-cancerous tissue. After 3 days cisplatin treatment, the survived Tca8113 cells had a lower expression of miR-203 than that in the untreated control group. In contrast, PIK3CA showed an inverse expression in cancer and cisplatin survived Tca8113 cells. Transfection of Tca8113 cells with miR-203 mimics greatly reduced PIK3CA expression and Akt activation. Furthermore, miR-203 repressed PIK3CA expression through targeting the 3′UTR. Restoration of miR-203 not only suppressed cell proliferation, but also sensitized cells to cisplatin induced cell apoptosis. This effect was absent in cells that were simultaneously treated with PIK3CA RNAi. In summary, these findings suggest miR-203 plays an important role in cisplatin resistance in OSCC, and furthermore delivery of miR-203 analogs may serve as an adjuvant therapy for OSCC. - Highlights: • Much lower miR-203 expression in cisplatin resistant Tca8113 cells is discovered. • Delivery of miR-203 can sensitize the Tca8113 cells to cisplatin induced cell death. • MiR-203 can downregulate PIK3CA through the 3′UTR. • The effects of miR-203 on cisplatin sensitivity is mainly through PIK3CA pathway.

  7. 17 CFR 275.203(b)(3)-1 - Definition of “client” of an investment adviser.

    Science.gov (United States)

    2010-04-01

    ... Definition of “client” of an investment adviser. Preliminary Note to § 275.203(b)(3)-1. This section is a... single client for purposes of section 203(b)(3) of the Act. Under paragraph (b)(6) of this section, the... be a single client for purposes of section 203(b)(3) of the Act (15 U.S.C. 80b-3(b)(3)): (1) A...

  8. Rewiring of an Epithelial Differentiation Factor, miR-203, to Inhibit Human Squamous Cell Carcinoma Metastasis

    Directory of Open Access Journals (Sweden)

    Nathan Benaich

    2014-10-01

    Full Text Available Summary: Metastatic colonization of distant organs underpins the majority of human-cancer-related deaths, including deaths from head and neck squamous cell carcinoma (HNSCC. We report that miR-203, a miRNA that triggers differentiation in multilayered epithelia, inhibits multiple postextravasation events during HNSCC lung metastasis. Inducible reactivation of miR-203 in already established lung metastases reduces the overall metastatic burden. Using an integrated approach, we reveal that miR-203 inhibits metastasis independently of its effects on differentiation. In vivo genetic reconstitution experiments show that miR-203 inhibits lung metastasis by suppressing the prometastatic activities of three factors involved in cytoskeletal dynamics (LASP1, extracellular matrix remodeling (SPARC, and cell metabolism (NUAK1. Expression of miR-203 and its downstream effectors correlates with HNSCC overall survival outcomes, indicating the therapeutic potential of targeting this signaling axis. : Benaich et al. have identified miR-203, a microRNA that triggers differentiation in multilayered epithelia, as an inhibitor of lung metastasis in head and neck squamous cell carcinoma (HNSCC cells. They show that miR-203 inhibits metastasis independently of its effects on differentiation. Rather, miR-203 suppresses the prometastatic activities of three factors involved in cytoskeletal dynamics (LASP1, extracellular matrix remodeling (SPARC, and cell metabolism (NUAK1. Expression of miR-203 and its downstream effectors correlates with survival in HNSCC patients.

  9. Consecutive Renal Scanning with {sup 131}I And {sup 203}Hg in Obstructive Uropathy

    Energy Technology Data Exchange (ETDEWEB)

    Poshyachinda, V. [Chulalongkorn Hospital, Bangkok (Thailand)

    1969-05-15

    Two patients with obstructive uropathy were scanned after injections of {sup 131}I sodium orthoiodohippurate (OlH) and {sup 203}Hg chlormerodrin. The {sup 131}I OIH scan was performed 30 min after the injection with the patient in the prone position. The {sup 203}Hg chlormerodrin was injected as soon as a negligible amount of {sup 131}I OIH was detected over the kidney, and the patient was scanned again 30 min later. A third patient and a normal subject were scanned 20 min after the simultaneous injection of {sup 131}I OIH and {sup 203}Hg chlormerodrin. Three scans with different analyser settings were done in sequence. The first scan was done with a window setting for {sup 131}I, and the second scan included both {sup 131}I and {sup 203}Hg; in the third scan, the analyser was set for {sup 203}Hg only. Evidence of hydronephroses, hydroureter and the residual functioning renal tissue were demonstrated by this scanning technique. The intravenous pyelograms in all three cases had failed to give satisfactory results. The simultaneous injection technique was found advantageous over the separate injection technique. (author)

  10. Investigation of parity admixtures in nuclear states by measuring the β-γ directional correlation of the 203Hg → 203Tl decay

    International Nuclear Information System (INIS)

    Dydak, F.

    1972-01-01

    As a consequence of a weak nucleon-nucleon interaction parity admixtures in nuclear states give rise to a small forward-backward asymmetry of γ-quanta with respect to the direction of β-particle emission. A highly accurate arrangement using four β- and two γ-detectors has been developed to determine the odd Legendre term A cos(theta) in the β-γ directional correlation of the cascade decay 203 Hg→ 203 Tl as well as a control value which should be zero. The electronic equipment is a specially designed nanosecond system. The sources were prepared by means of a mass separator. Possible systematic distortions of the experimental data were carefully examined. A generalized least squares fit method symmetric in all variables was deduced for a correct data evaluation. The measurements yielded the coefficient A 1 = -(2,7+-0,7).10 -4 and the control value +(0,7+-0,8).10 -4 . (author)

  11. Use of Tesla NZK 203 neutron probe in studying water as geochemical factor in weathering zone

    International Nuclear Information System (INIS)

    Hally, J.

    1980-01-01

    The Tesla NZK 203 neutron moisture gage was used for determining soil moisture in depths of 1 and 2 meters in the Luha-Kacerov profile in the Zelivka water reservoir basin. The borehole spacing was 100 m. Measurements were made once a month between May and October 1978. The results were compared with the granulometric composition and the values of the specific surface areas of earths in the area. The Tesla NZK 203 moisture gage was fully proven for speedy measurement of volume moisture at constant measuring points. (Ha)

  12. Technical evaluation of Tom Scurry Associates: Model PM-203 doorway monitor

    International Nuclear Information System (INIS)

    1978-07-01

    Under a basic assignment by the Office of Safeguards and Security, the Tom Scurry Associates Model PM-203 Personnel Doorway SNM Monitor manufactured by Tom Scurry Associates was evaluated by LASL Group Q-2 against the DOE Personnel Doorway Monitor standards. During the evaluation, a small change was required in detector shielding to eliminate low sensitivity areas at the portal sides. With the modified shielding described, the PM-203 meets the Office of Safeguards and Security SNM doorway monitor specifications for detecting either 235 U or 239 Pu-- 233 U. This system is also capable of monitoring 238 Pu

  13. The effects of thiamin on lead metabolism: organ distribution of lead 203.

    OpenAIRE

    Kim, J S; Hamilton, D L; Blakley, B R; Rousseaux, C G

    1992-01-01

    The effect of thiamin on the organ distribution of lead was evaluated in CD-1 mice exposed intragastrically or intraperitoneally to a single dose of lead acetate (100 micrograms) containing 100 microCi lead 203. They were treated with either thiamin (25 or 50 mg/kg body weight), calcium ethylenediaminetetraacetic acid (CaEDTA) (50 mg/kg body weight), or combinations of thiamin and CaEDTA. The whole body retention and the organ distribution of lead 203 varied depending upon the route of lead a...

  14. Investigation of parity mixing in nuclear states by measurement of beta-gamma directional correlations at the transition 203Hg- 203Tl and a control experiment hereto

    International Nuclear Information System (INIS)

    Serentschy, G.

    1978-01-01

    Models of the Weak Interaction Hamiltonian predict a variety of parity-non-conserving (PNC) effects whose typical feature is odd transformation behavior under space reflection. The investigation of these effects is the task of the so-called parity experiments. Remeasurement of a PNC-effect at the 279 keV level in Tl-203 and the realization of a control experiment with the same apparatus to get information about possible systematic errors in it, are the subject of the submitted dissertation. Furthermore all relevant existing methods and some exciting trends in the field of PNC-experiments are extensively discussed with the aim of a better understanding of the present experimental situation, the prospects and especially the significance of this experiment at Tl-203. It could be demonstrated, that PNC-experiments on heavy nuclei (such as Tl-203) are less important now than they were five years ago, because of the insufficient understanding of the structure of heavy nuclei on the one hand, and the increasing importance (caused by technological improvements) of PNC-experiments at much better calculable systems, such as few nucleon systems and light atoms (muonic or electronic), on the other hand. The preliminary result of this control experiment is not clear enough for drawing final conclusions yet, particularly it is not possible, taking this preliminary result as a basis, to prove our apparatus either to be free of systematic errors or not. (author)

  15. 12 CFR Appendix B to Part 203 - Form and Instructions for Data Collection on Ethnicity, Race, and Sex

    Science.gov (United States)

    2010-01-01

    ... Ethnicity, Race, and Sex B Appendix B to Part 203 Banks and Banking FEDERAL RESERVE SYSTEM BOARD OF... to Part 203—Form and Instructions for Data Collection on Ethnicity, Race, and Sex I. Instructions on Collection of Data on Ethnicity, Race, and Sex You may list questions regarding the ethnicity, race, and sex...

  16. 9 CFR 381.203 - Products offered for entry; charges for storage, cartage, and labor with respect to products...

    Science.gov (United States)

    2010-01-01

    ... CERTIFICATION POULTRY PRODUCTS INSPECTION REGULATIONS Imported Poultry Products § 381.203 Products offered for... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Products offered for entry; charges for storage, cartage, and labor with respect to products which are refused entry. 381.203 Section 381...

  17. 42 CFR 137.203 - May a Self-Governance Tribe participate in a voluntary national uniform data collection effort...

    Science.gov (United States)

    2010-10-01

    ...-Governance Tribe participate in a voluntary national uniform data collection effort with the IHS? Yes, in... 42 Public Health 1 2010-10-01 2010-10-01 false May a Self-Governance Tribe participate in a voluntary national uniform data collection effort with the IHS? 137.203 Section 137.203 Public Health PUBLIC...

  18. 13 CFR 124.203 - What must a concern submit to apply to the 8(a) BD program?

    Science.gov (United States)

    2010-01-01

    ... to, financial statements, Federal personal and business tax returns, and personal history statements... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What must a concern submit to apply to the 8(a) BD program? 124.203 Section 124.203 Business Credit and Assistance SMALL BUSINESS...

  19. 5 CFR 2641.203 - One-year restriction on any former employee's representations, aid, or advice concerning ongoing...

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false One-year restriction on any former employee's representations, aid, or advice concerning ongoing trade or treaty negotiation. 2641.203 Section 2641.203 Administrative Personnel OFFICE OF GOVERNMENT ETHICS GOVERNMENT ETHICS POST-EMPLOYMENT CONFLICT OF INTEREST RESTRICTIONS Prohibitions §...

  20. 16 CFR Appendix D to Part 436 - Sample Item 20(3) Table-Status of Franchise Outlets

    Science.gov (United States)

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Sample Item 20(3) Table-Status of Franchise Outlets D Appendix D to Part 436 Commercial Practices FEDERAL TRADE COMMISSION TRADE REGULATION RULES... Item 20(3) Table—Status of Franchise Outlets Status of Franchise Outlets For years 2004 to 2006 Column...

  1. 9 CFR 203.14 - Statement with respect to advertising allowances and other merchandising payments and services.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Statement with respect to advertising allowances and other merchandising payments and services. 203.14 Section 203.14 Animals and Animal Products... to advertising allowances and other merchandising payments and services. The Guidelines 1. Who is a...

  2. 24 CFR 203.16 - Certificate and contract regarding use of dwelling for transient or hotel purposes.

    Science.gov (United States)

    2010-04-01

    ... use of dwelling for transient or hotel purposes. 203.16 Section 203.16 Housing and Urban Development... use of dwelling for transient or hotel purposes. Every application filed with respect to insurance of... housing or any part thereof covered by the mortgage for transient or hotel purposes, together with the...

  3. 17 CFR 275.203(b)(3)-2 - Methods for counting clients in certain private funds.

    Science.gov (United States)

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Methods for counting clients....203(b)(3)-2 Methods for counting clients in certain private funds. (a) For purposes of section 203(b)(3) of the Act (15 U.S.C. 80b-3(b)(3)), you must count as clients the shareholders, limited partners...

  4. 9 CFR 203.5 - Statement with respect to market agencies paying the expenses of livestock buyers.

    Science.gov (United States)

    2010-01-01

    ... commission basis, to pay certain of the business or personal expenses incurred by buyers attending livestock... commission basis, to pay, directly or indirectly, any personal or business expenses of livestock buyers... agencies paying the expenses of livestock buyers. 203.5 Section 203.5 Animals and Animal Products GRAIN...

  5. 31 CFR 585.203 - Holding of certain types of blocked property in interest-bearing accounts.

    Science.gov (United States)

    2010-07-01

    ... property in interest-bearing accounts. 585.203 Section 585.203 Money and Finance: Treasury Regulations... blocked property in interest-bearing accounts. (a)(1) Any person, including a U.S. financial institution... subsequent to July 15, 1992, is not being held in an interest-bearing account or otherwise invested in a...

  6. 31 CFR 595.203 - Holding of certain types of blocked property in interest-bearing accounts.

    Science.gov (United States)

    2010-07-01

    ... property in interest-bearing accounts. 595.203 Section 595.203 Money and Finance: Treasury Regulations... interest-bearing accounts. (a)(1) Any person, including a U.S. financial institution, currently holding... effective date, is not being held in an interest-bearing account, or otherwise invested in a manner...

  7. 31 CFR 575.203 - Holding of certain types of blocked property in interest-bearing accounts.

    Science.gov (United States)

    2010-07-01

    ... property in interest-bearing accounts. 575.203 Section 575.203 Money and Finance: Treasury Regulations... interest-bearing accounts. (a) Any person, including a U.S. financial institution, currently holding... effective date, is not being held in an interest-bearing account, or otherwise invested in a manner...

  8. 31 CFR 536.203 - Holding of certain types of blocked property in interest-bearing accounts.

    Science.gov (United States)

    2010-07-01

    ... property in interest-bearing accounts. 536.203 Section 536.203 Money and Finance: Treasury Regulations... property in interest-bearing accounts. (a)(1) Any person, including a U.S. financial institution, currently... to the effective date, is not being held in an interest-bearing account, or otherwise invested in a...

  9. 13 CFR 127.203 - What are the rules governing the requirement that economically disadvantaged women must own EDWOSBs?

    Science.gov (United States)

    2010-01-01

    ... requirement that economically disadvantaged women must own EDWOSBs? 127.203 Section 127.203 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION WOMEN-OWNED SMALL BUSINESS FEDERAL CONTRACT ASSISTANCE... woman if the transfer was: (1) To or on behalf of an immediate family member for that individual's...

  10. Brain, kidney and liver 203Hg-methyl mercury uptake in the rat: Relationship to the neutral amino acid carrier

    International Nuclear Information System (INIS)

    Aschner, M.

    1989-01-01

    To investigate the effect of L-neutral amino acids on tissue levels of methyl mercury in the adult animal, rats were infused into the external jugular vein with solutions containing a) 0.05 mM 203 Hg-MeHgCl and saline, b) 0.05 mM 203 Hg-MgHgCl-0.1 mM L-cysteine, c) 0.05 mM 203 Hg-MeHgCl-0.1 mM L-cysteine-0.1 mM L-methionine, d) 0.05 mM 203 Hg-MeHgCl-0.1 mM L-leucine, or e) 0.05 mM 203 Hg-MeHgCl-0.1 mM L-cysteine-0.1 mM L-leucine. Groups of animals were sacrificed at 3 min. 7 hr, and 96 hr. Brain, kidney, and liver 203 Hg radioactivity was measured by means of gamma-scintillation spectrometry. Brain 203 Hg concentrations L-cysteine treated animals were significantly higher compared with saline treated animals (P 203 Hg uptake (P 203 Hg concentrations were not significantly different in any of the treatment groups compared with controls, irrespective of the sacrifice time. Furthermore, the percentage of diffusible 203 Hg (non-protein bound) at each sacrifice time was not statistically different irrespective of the treatment assigned. These results suggest that methyl mercury L-cysteine conjugates in the plasma may share a common transport step with the L-neutral amino acid carrier transport system and indicate the presence in brain capillaries of a transport system capable of selectively mediating methyl mercury uptake across the capillary endothelial cell membrane. (author)

  11. CNS histopathology on 203 bovines with clinical suspicion of BSE in Denmark 2001 to 2016

    DEFF Research Database (Denmark)

    Jensen, Tim Kåre

    2017-01-01

    During 2001 to 2016 a total of 203 bovines were submitted to the instutute with clinical suspicion of having BSE. In two cases BSE was confirmed. The most common differential diagnosis was listeriosis, found in 54% of the cases. Listeriosis was characterized by multifocal, necrotizing, non-suppur...

  12. 24 CFR 982.203 - Special admission (non-waiting list): Assistance targeted by HUD.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Special admission (non-waiting list... Admission to Tenant-Based Program § 982.203 Special admission (non-waiting list): Assistance targeted by HUD... family residing in a multifamily rental housing project when HUD sells, forecloses or demolishes the...

  13. 21 CFR 203.39 - Donation of drug samples to charitable institutions.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 4 2010-04-01 2010-04-01 false Donation of drug samples to charitable... SERVICES (CONTINUED) DRUGS: GENERAL PRESCRIPTION DRUG MARKETING Samples § 203.39 Donation of drug samples... donation record accurately describes the drug sample delivered and that no drug sample is adulterated or...

  14. 33 CFR 203.42 - Inspection of non-Federal flood control works.

    Science.gov (United States)

    2010-07-01

    ... PROCEDURES Rehabilitation Assistance for Flood Control Works Damaged by Flood or Coastal Storm: The Corps Rehabilitation and Inspection Program § 203.42 Inspection of non-Federal flood control works. (a) Required... will conduct an IEI to determine if the flood control work meets minimum engineering and maintenance...

  15. 30 CFR 204.203 - What is the other relief option?

    Science.gov (United States)

    2010-07-01

    ... MANAGEMENT ALTERNATIVES FOR MARGINAL PROPERTIES Accounting and Auditing Relief § 204.203 What is the other... that is appropriate for production from your marginal property, provided it is not prohibited under... are: (1) To report and pay royalties using a valuation method other than that required under 30 CFR...

  16. 33 CFR 203.14 - Responsibilities of non-Federal interests.

    Science.gov (United States)

    2010-07-01

    ... Introduction § 203.14 Responsibilities of non-Federal interests. Non-Federal interests, which include State... Program (RIP), as detailed in subpart D of this part; and, (5) Responsible regulation, management, and use... participation may include either financial contribution or commitment of non-Federal physical resources, or both. ...

  17. 48 CFR 6.203 - Set-asides for small business concerns.

    Science.gov (United States)

    2010-10-01

    ... 6.203 Set-asides for small business concerns. (a) To fulfill the statutory requirements relating to small business concerns, contracting officers may set aside solicitations to allow only such business concerns to compete. This includes contract actions conducted under the Small Business Innovation Research...

  18. 30 CFR 203.3 - Do I have to pay a fee to request royalty relief?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false Do I have to pay a fee to request royalty... MINERALS REVENUE MANAGEMENT RELIEF OR REDUCTION IN ROYALTY RATES General Provisions § 203.3 Do I have to pay a fee to request royalty relief? When you submit an application or ask for a preview assessment...

  19. 17 CFR 275.203-1 - Application for investment adviser registration.

    Science.gov (United States)

    2010-04-01

    ... must file electronically with the Investment Adviser Registration Depository (IARD), unless you have... you have paid the filing fee. [65 FR 57448, Sept. 22, 2000; 65 FR 81737, Dec. 27, 2000; 68 FR 42248... EXCHANGE COMMISSION (CONTINUED) RULES AND REGULATIONS, INVESTMENT ADVISERS ACT OF 1940 § 275.203-1...

  20. 24 CFR 203.477 - Certificate by lender when loan assigned.

    Science.gov (United States)

    2010-04-01

    ... AUTHORITIES SINGLE FAMILY MORTGAGE INSURANCE Contract Rights and Obligations Rehabilitation Loans § 203.477... a good right to assign. (c) The mortgage transaction did not involve a first mortgage and the mortgage is prior to all mechanics' and materialmen's liens filed of record, regardless of when such liens...

  1. 75 FR 45144 - Recovery Fact Sheet 9580.203, Debris Monitoring

    Science.gov (United States)

    2010-08-02

    ... DEPARTMENT OF HOMELAND SECURITY Federal Emergency Management Agency [Docket ID FEMA-2010-0045] Recovery Fact Sheet 9580.203, Debris Monitoring AGENCY: Federal Emergency Management Agency, DHS. ACTION: Notice of availability; request for comments. SUMMARY: The Federal Emergency Management Agency (FEMA) is...

  2. 22 CFR 203.5 - U.S. PVO annual documentation requirements.

    Science.gov (United States)

    2010-04-01

    ....5 Section 203.5 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT REGISTRATION OF PRIVATE..., or bylaws and any changes in the tax-exempt status. (c) Submission is due within six months after the... must be sent annually to the USAID Registrar, Office of Private Voluntary Cooperation—American Schools...

  3. 6 CFR 27.203 - Calculating the screening threshold quantity by security issue.

    Science.gov (United States)

    2010-01-01

    ... SECRETARY CHEMICAL FACILITY ANTI-TERRORISM STANDARDS Chemical Facility Security Program § 27.203 Calculating... possesses a chemical of interest that meets the STQ for any security issue, a facility need not include... development, involving chemicals of interest conducted outside the laboratory. (ii) [Reserved] (3) Propane. In...

  4. 31 CFR 203.4 - Financial institution eligibility for designation as a TT&L depositary.

    Science.gov (United States)

    2010-07-01

    ... PAYMENT OF FEDERAL TAXES AND THE TREASURY TAX AND LOAN PROGRAM General Information § 203.4 Financial... secure TT&L account balances, a TIP main account balance, an SDI account balance, or a no account balance... Federal tax deposits through PATAX, a financial institution must possess under its charter either general...

  5. 76 FR 63602 - Voting Rights Act Amendments of 2006, Determinations Under Section 203

    Science.gov (United States)

    2011-10-13

    ... subdivisions obligated to comply with the requirements are listed in the attachment to this Notice. Section 203..., where a state is covered, those counties or county equivalents not displayed in the attachment are... Hispanic. Maryland: Montgomery County Hispanic. Massachusetts: Boston city Hispanic. Chelsea city Hispanic...

  6. 31 CFR 0.203 - Gifts or gratuities from foreign governments.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Gifts or gratuities from foreign... DEPARTMENT OF THE TREASURY EMPLOYEE RULES OF CONDUCT Rules of Conduct § 0.203 Gifts or gratuities from foreign governments. (a) The United States Constitution prohibits employees from accepting gifts...

  7. 5 CFR 720.203 - Responsibilities of the Office of Personnel Management.

    Science.gov (United States)

    2010-01-01

    ... Recruitment Program § 720.203 Responsibilities of the Office of Personnel Management. (a) The Office of... underrepresentation. The process for making such determinations is described in sections II and III (c) of the “Guidelines for the Development of a Federal Recruitment Program to Implement 5 U.S.C. 7201, as amended” (See...

  8. 24 CFR 3280.203 - Flame spread limitations and fire protection requirements.

    Science.gov (United States)

    2010-04-01

    ... value shown in § 3280.203(b) when tested by Standard Test Method for Surface Burning Characteristics of Building Materials, ASTM E84-01, 2001, or Standard Method of Test of Surface Burning Characteristics of... plastic panel countertop; (ii) 1/4-inch or thicker unfinished plywood with phenolic or urea glue; (iii...

  9. Chemical recovery of thallium-203 following production and separation of lead-201

    International Nuclear Information System (INIS)

    Kayfus, G.P.; Boothe, T.E.; Campbell, J.A.; Finn, R.D.; Gilson, A.J.

    1982-01-01

    Because of the cost and limited availabilty of isotopically enriched thallium (>92% 203 Tl), its use in the 203 Tl(p, 3n) 201 Pb nuclear reaction necessitates chemical recovery. An adaptive method has been developed and evaluated. After the separation of 201 Pb, the 203 Tl(I) is oxidized to 203 Tl(III) by Cl 2 , Br 2 or [Fe(CN) 6 ] -2 , precipitated as Tl(OH) 3 with NaOH and subsequently converted to Tl 2 O 3 by heating. Due to potential loss during recovery, the solubilities of Tl(OH) 3 and Tl 2 O 3 in aqueous solution as a function of pH have been studied using the internal tracer 202 Tl(T=12.2 d), produced during cyclotron irradiation. Effective solubility product constants have been determined to be 5.4x10 -48 and 2.5x10 -47 for Tl(OH) 3 and Tl 2 O 3 , respectively. (author)

  10. 30 CFR 203.5 - What is MMS's authority to collect information?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What is MMS's authority to collect information... REVENUE MANAGEMENT RELIEF OR REDUCTION IN ROYALTY RATES General Provisions § 203.5 What is MMS's authority....” (b) The MMS collects this information to make decisions on the economic viability of leases...

  11. 33 CFR 203.61 - Emergency water supplies due to contaminated water source.

    Science.gov (United States)

    2010-07-01

    .... (5) Loss of water supply is not a basis for assistance under this authority. (6) Water will not be... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Emergency water supplies due to... PROCEDURES Emergency Water Supplies: Contaminated Water Sources and Drought Assistance § 203.61 Emergency...

  12. 30 CFR 203.74 - When will MMS reconsider its determination?

    Science.gov (United States)

    2010-07-01

    ... for Pre-Act Deep Water Leases and for Development and Expansion Projects § 203.74 When will MMS... information (but not reinterpreting old data); (2) Did not exist at the time of the earlier application; and... equivalent market conditions, of the field or lease relative to the development system proposed in the prior...

  13. 31 CFR 594.203 - Holding of funds in interest-bearing accounts; investment and reinvestment.

    Science.gov (United States)

    2010-07-01

    ... tangible property, such as chattels or real estate, or of other blocked property, such as debt or equity... GLOBAL TERRORISM SANCTIONS REGULATIONS Prohibitions § 594.203 Holding of funds in interest-bearing...). However, the Office of Foreign Assets Control may issue licenses permitting or directing such sales or...

  14. 24 CFR 972.203 - Definition of “conversion.”

    Science.gov (United States)

    2010-04-01

    ... DEVELOPMENT CONVERSION OF PUBLIC HOUSING TO TENANT-BASED ASSISTANCE Voluntary Conversion of Public Housing Developments Purpose; Definition of Conversion § 972.203 Definition of “conversion.” For purposes of this subpart, the term “conversion” means the removal of public housing units from the inventory of a Public...

  15. 24 CFR 203.281 - Calculation of one-time MIP.

    Science.gov (United States)

    2010-04-01

    ... HOUSING AND URBAN DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES SINGLE FAMILY MORTGAGE INSURANCE Contract Rights and Obligations Mortgage Insurance Premiums-One-Time Payment § 203.281 Calculation of one-time MIP. (a) The applicable premium percentage determined...

  16. 24 CFR 203.268 - Pro rata payment of periodic MIP.

    Science.gov (United States)

    2010-04-01

    ... HOUSING AND URBAN DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES SINGLE FAMILY MORTGAGE INSURANCE Contract Rights and Obligations Mortgage Insurance Premiums-Periodic Payment § 203.268 Pro rata payment of periodic MIP. (a) If the insurance contract is terminated...

  17. 24 CFR 203.22 - Payment of insurance premiums or charges; prepayment privilege.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Payment of insurance premiums or... Underwriting Procedures Eligible Mortgages § 203.22 Payment of insurance premiums or charges; prepayment privilege. (a) Payment of periodic insurance premiums or charges. Except with respect to mortgages for which...

  18. Basophil sensitivity through CD63 or CD203c is a functional measure for specific immunotherapy

    Directory of Open Access Journals (Sweden)

    Dahl Ronald

    2010-02-01

    Full Text Available Abstract Background Subcutaneous Immunotherapy (SCIT modifies the allergic response and relieves allergic symptoms. SCIT is the only and a very effective treatment for insect venom allergy. We hypothesized that basophil sensitivity, measured through the basophil activation test, would decrease during SCIT up dosing. Expression of CD203c was compared to CD63 as marker for basophil activation, using a Bland Altman plot and ROC curves. Methods Patients (n = 18 starting subcutaneous SCIT for wasp allergy with an up dosing scheme of 7 to 11 weeks were enrolled. Heparinised blood samples were drawn at weeks 1-4, 7 and at the first maintenance visit. Basophils were stimulated at 7 log dilutions of V. vespula allergen for 15 min, and were stained with CD203c and CD63. Basophils were identified as CD203c+ leukocytes, and the proportion of CD63+ and CD203c+ cells were plotted against allergen concentration. A sigmoid curve was fitted to the points, and the allergen concentration at which half of the maximal activation was achieved, LC50, was calculated. In another series of experiments, LC50 calculated in whole blood (AP was subtracted from LC50 calculated with basophils suspended in plasma from a nonatopic donor (HS to determine the protective effect of soluble factors in blood of patients treated with SCIT. Results Heparin blood basophil activation was similar through CD63 and CD203c. Basophils were significantly more sensitized three weeks after initiation of SCIT compared to baseline (p Conclusion Basophil activation is a versatile and sensitive tool that measures changes in the humoral immune response to allergen during SCIT.

  19. miR-203a is involved in HBx-induced inflammation by targeting Rap1a

    Energy Technology Data Exchange (ETDEWEB)

    Wu, AiRong [Department of gastroenterology, The First affiliated Hospital of Soochow University, Suzhou 215006 (China); Chen, Huo [Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123 (China); Xu, ChunFang [Department of gastroenterology, The First affiliated Hospital of Soochow University, Suzhou 215006 (China); Zhou, Ji; Chen, Si [Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123 (China); Shi, YuQi [Department of gastroenterology, The First affiliated Hospital of Soochow University, Suzhou 215006 (China); Xu, Jie [Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123 (China); Gan, JianHe, E-mail: j_pzhang@suda.edu.cn [Department of gastroenterology, The First affiliated Hospital of Soochow University, Suzhou 215006 (China); Zhang, JinPing, E-mail: ganjianhe@aliyun.com [Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123 (China)

    2016-11-15

    Hepatitis B virus (HBV) causes acute and chronic hepatitis, and is one of the major causes of cirrhosis and hepatocellular carcinoma. Accumulating evidence suggests that inflammation is the key factor for liver cirrhosis and hepatocellular carcinoma. MicroRNAs play important roles in many biological processes. Here, we aim to explore the function of microRNAs in the HBX-induced inflammation. First, microarray experiment showed that HBV{sup +} liver samples expressed higher level of miR-203a compared to HBV{sup -} liver samples. To verify these alterations, HBx-coding plasmid was transfected into HepG2 cells to overexpress HBx protein. The real-time PCR results suggested that over-expression of HBx could induce up-regulation of miR-203a. To define how up-regulation of miR-203a can induce liver cells inflammation, we over-expressed miR-203a in HepG2 cells. Annexin V staining and BrdU staining suggested that overexpression of miR-203a significantly increased the cell apoptosis and proliferation, meanwhile, over-expression of miR-203a could lead to a decrease in G0/G1 phase cells and an increase in G2/M phase cells. Some cytokines production including IL-6 and IL-8 were significantly increased, but TGFβ and IFNγ were decreased in miR-203a over-expressed HepG2 cells. Luciferase reporter assay experiments, protein mass-spectrum assay and real-time PCR all together demonstrated that Rap1a was the target gene of miR-203a. Further experiments showed that these alterations were modulated through PI3K/ERK/p38/NFκB pathways. These data suggested that HBV-infection could up-regulate the expression of miR-203a, thus down regulated the expression of Rap1a and affected the PI3K/ERK/p38/NFκB pathways, finally induced the hepatitis inflammation. - Highlights: • HBX induces the over-expression of miR-203a in HepG2 cells. • miR-203a targets Rap1a to induce the inflammation in HepG2 cells. • miR-203a regulates the apoptosis and cell cycles of HepG2 cells. • miR-203a alters

  20. Mercury 203 distribution in pregnant and nonpregnant rats following systemic infusions with thiol-containing amino acids

    International Nuclear Information System (INIS)

    Aschner, M.; Clarkson, T.W.

    1987-01-01

    Near-term pregnant (gestational day 17) and nonpregnant Long-Evans female rats were continuously infused into the external jugular vein with 0.1 mmole/hour L-cysteine, 0.1 mmole/hour L-leucine, or saline. At 24, 48, and 72 hours, 50 mumole/hour [ 203 Hg]-MeHgCl was administered over 1 hour. Total 203 Hg body burden, brain, kidney, liver, and blood 203 Hg concentrations were determined at 96 hours by gamma scintillation spectrometry. Despite significantly greater 203 Hg whole body retention in the pregnant animals 203 Hg concentrations in blood, brain, kidney, and liver were higher in nonpregnant rats. In addition, brain 203 Hg concentrations in both pregnant and virgin rats were significantly higher in L-cysteine-treated rats compared with controls. These results suggest that the fetus may act as a sink for MeHg, thus decreasing 203 Hg concentrations in maternal blood, brain, kidney, and liver. Furthermore, the data indicate that brain uptake of methylmercury in both pregnant and nonpregnant rats is enhanced by chronic L-cysteine infusion, lending support to the hypothesis that methylmercury in the rat may be translocated across the blood-brain barrier by the neutral amino acid carrier transport system

  1. Astatine-211 labelled proteins and their stability in vivo

    International Nuclear Information System (INIS)

    Yi Changhou; Jin Jannan; Zhang Shuyuan; Wang Ketai; Zhang Dayuan; Zhou Maolun

    1989-01-01

    211 At or 131 I labelled proteins, e.g. 211 At-IgG or 211 At-BSA (bovine serum albumin) were prepared by 211 At reaction with the diazo-compound of para-aminobenzoic acid, which is then conjugated with IgG or BSA via an acylation reaction. The 211 At-carbon bond was found metabolically stable under in vivo conditions. For the labelling of proteins with 211 At or 131 I, other methods of direct oxidation are also described. The results show that for the labelling of proteins with 211 At, high rate of incorporation can be obtained with hydrogen peroxide as oxidant, but the labelling of proteins with 131 I is more favourable with the strong oxidant Chloramine-T. (author) 12 refs.; 6 figs

  2. Microanalysis of oligosaccharide HS203 in beagle dog plasma by postcolumn fluorescence derivatization method.

    Science.gov (United States)

    Sun, Shumeng; Zhao, Xia; Li, Guangsheng; Yu, Guangli; Xing, Xiaoxu; Zeng, Yangyang; Wu, Jian; Wang, Jianing

    2012-06-20

    A rapid and sensitive postcolumn fluorescence derivatization method was developed for microanalysis of antidiabetic oligosaccharide HS203 in beagle dog plasma. After plasma protein was removed by a simple and fast ultrafiltration method, chromatographic separation was performed on an Asahipak GS-320 HQ column with a mobile phase of 50 mmol/L phosphate buffer (pH 6.7) and acetonitrile (83/17, v/v). The column effluent was monitored by fluorescence detection at 249 nm (excitation) and 435 nm (emission) using guanidine hydrochloride as a postcolumn derivatizing reagent. A satisfactory resolution of the analyte was achieved and the limit of detection was found to be 4 ng (more sensitive than silver staining of HS203 in polyacrylamide gel electrophoresis). The method described above was successfully applied to a pharmacokinetic study of HS203 and to monitor blood glucose level simultaneously in beagle dog. It is also possible to be applied for microanalysis of other oligosaccharides in biological samples. Copyright © 2012 Elsevier Ltd. All rights reserved.

  3. Tank 241-T-203, core 190 analytical results for the final report

    International Nuclear Information System (INIS)

    Steen, F.H.

    1997-01-01

    This document is the analytical laboratory report for tank 241-T-203 push mode core segments collected on April 17, 1997 and April 18, 1997. The segments were subsainpled and analyzed in accordance with the Tank 241-T-203 Push Mode Core Sampling andanalysis Plan (TSAP) (Schreiber, 1997a), the Safety Screening Data Quality Objective (DQO)(Dukelow, et al., 1995) and Leffer oflnstructionfor Core Sample Analysis of Tanks 241-T-201, 241-T-202, 241-T-203, and 241-T-204 (LOI)(Hall, 1997). The analytical results are included in the data summary report (Table 1). None of the samples submitted for Differential Scanning Calorimetry (DSC), Total Alpha Activity (AT) and Total Organic Carbon (TOC) exceeded notification limits as stated in the TSAP (Schreiber, 1997a). The statistical results of the 95% confidence interval on the mean calculations are provided by the Tank Waste Remediation Systems (TWRS) Technical Basis Group in accordance with the Memorandum of Understanding (Schreiber, 1997b) and not considered in this report

  4. Influence of spironolactone on the excretion of 203Hg2+ in rats

    International Nuclear Information System (INIS)

    Cikrt, M.; Tichy, M.

    1975-01-01

    The effect of spironolactone (SPL) on 203 Hg 2+ excretion was studied in rats with a special emphasis in biliary excretion. No correlation was found between the number of doses of SPL pretreatment (1-5 doses of 5 mg/100 g body weight) and the biliary excretion of 203 Hg 2+ within 6 hours after intravenous administration of 120 μg Hg 2+ per rat. After the SPL pretreatment there was a significantly increased mercury stool excretion 24 hours after intravenous administration. Concurrent oral administration of SH-groups containing sorbent had no effect on mercury stool excretion. Repeated administration of 203 Hg 2+ (5 hours after the first dose) induced significantly increased biliary excretion of mercury in rats pretreated with SPL. On the other hand, repeated administration of SPL (4 hours after intravenous administration of mercury) did not influence the biliary excretion of mercury. The results indicate that the effect of SPL on biliary excretion of mercury could be limited by the level of ''mercury available'' in the organism and might be determined by a direct interaction of mercury molecule with the molecule of SPL

  5. Development of a new radiolabel (lead-203) and new chelating agents for labeling monoclonal anntibodies for imaging

    International Nuclear Information System (INIS)

    Srivastava, S.C.; Mease, R.C.; Meinken, G.E.; Mausner, L.F.; Steplewski, Z.

    1988-01-01

    High liver uptake and slow body clearance presently limit the usefulness of 111 In labeled antibodies for tumor imaging. We have investigated 203 Pb as an alternate and better antibody label. The DTPA and cyclohexyl EDTA (CDTA) conjugates of an anticolon carcinoma antibody, 17-1A were labeled (bicyclic anhydride method) with 203 Pb and 111 In with 60 and 90% labeling yields, respectively. The biodistribution of 203 Pb-17-1A conjugates was compared with the corresponding 111 In-labeled preparations and with 203 Pb-DTPA, 203 Pb-nitrate and nonrelevant antibody controls in normal and human tumor (SW948) xenografted nude mice at 24, and 96 hr. Lead-203-labeled CDTA and DTPA antibody conjugates gave similar in vivo distributions. Even though the lead bound to these chelate-antibody conjugates was more labile in serum and in vivo, compared to indium, it cleared much faster from the liver and the whole body. A new series of chelating agents based on the incorporation of a trans-1,2- diaminocyclohexane moiety into the carbon backbone of polyaminocarboxylates is being synthesized. These are expected to provide stronger complexing ability for lead and produce greater in vivo stability. These ligands are also expected to be superior to EDTA and DTPA for labeling antibodies with other radiometals, including indium. 32 refs., 3 tabs

  6. Production and radiochemical separation of 203Pb radioisotope for nuclear medicine

    International Nuclear Information System (INIS)

    Szuecs, Z.; Takacs, S.; Andrasi, D.; Kovacs, B.

    2012-01-01

    Complete text of publication follows. The heavy metal pollution due to their industrial production, waste repository or accident as the cyanide spill in river Tisza in 2002, increase the scientific interest for using an ideal trace isotope for monitoring these type of events. The lead is one of the most toxic and commonly used heavy metal, its poisoning is often deadly because very difficult to recognize and identify. The neuro-scientific study of biodegradation effect of lead could be an impressive scientific field of application of 203 Pb radioisotope. However the targeted radionuclide therapy especially the α-emitting radioisotope therapy is also strongly interested to find an ideal tracer for the 213 Bi and 212 Pb therapy. Therefore the 203 Pb is a potential radioisotope for this role due to its radiation behaviour and as heavy metal element. The 203 Tl(p,n) 203 Pb nuclear reaction was chosen for the production. The irradiation was done at the compact cyclotron of Atomki with proton beam 14.5 MeV energy and beam current of 7 μAs. The thickness of the target material was 840 μm, the irradiation time was 3 hours and the produced activity was 40 MBq at EOB. It corresponds to 1.87 MBq/μAh physical yield of the reaction which correlating with the cross section curve. A new technique was developed for target preparation. The metal Tl was pressed into a copper backing and covered with a HAWAR foil with thickness of 11 μm. The covering foil saved the surface of the Tl from the oxidation and also transferred the dissipating heat to the cooling He gas. The back side of the target was cooled with pressured cold water. The irradiated Tl target was pressed out from the copper backing, which had only the thickness of 0.2 mm. Then the Thallium was dissolved in nitric acid. The excess acid was evaporated slowly. The nitrate form was transferred to chloride form by 8 mol/dm 3 HCl and the Thallium was kept in 3+ oxidation stage by hydrogen peroxide. The separation was

  7. A novel network integrating a miRNA-203/SNAI1 feedback loop which regulates epithelial to mesenchymal transition.

    Directory of Open Access Journals (Sweden)

    Michèle Moes

    Full Text Available BACKGROUND: The majority of human cancer deaths are caused by metastasis. The metastatic dissemination is initiated by the breakdown of epithelial cell homeostasis. During this phenomenon, referred to as epithelial to mesenchymal transition (EMT, cells change their genetic and trancriptomic program leading to phenotypic and functional alterations. The challenge of understanding this dynamic process resides in unraveling regulatory networks involving master transcription factors (e.g. SNAI1/2, ZEB1/2 and TWIST1 and microRNAs. Here we investigated microRNAs regulated by SNAI1 and their potential role in the regulatory networks underlying epithelial plasticity. RESULTS: By a large-scale analysis on epithelial plasticity, we highlighted miR-203 and its molecular link with SNAI1 and the miR-200 family, key regulators of epithelial homeostasis. During SNAI1-induced EMT in MCF7 breast cancer cells, miR-203 and miR-200 family members were repressed in a timely correlated manner. Importantly, miR-203 repressed endogenous SNAI1, forming a double negative miR203/SNAI1 feedback loop. We integrated this novel miR203/SNAI1 with the known miR200/ZEB feedback loops to construct an a priori EMT core network. Dynamic simulations revealed stable epithelial and mesenchymal states, and underscored the crucial role of the miR203/SNAI1 feedback loop in state transitions underlying epithelial plasticity. CONCLUSION: By combining computational biology and experimental approaches, we propose a novel EMT core network integrating two fundamental negative feedback loops, miR203/SNAI1 and miR200/ZEB. Altogether our analysis implies that this novel EMT core network could function as a switch controlling epithelial cell plasticity during differentiation and cancer progression.

  8. MiR-203 controls proliferation, migration and invasive potential of prostate cancer cell lines

    DEFF Research Database (Denmark)

    Viticchiè, Giuditta; Lena, Anna Maria; Latina, Alessia

    2011-01-01

    Prostate cancers show a slow progression from a local lesion (primary tumor) to a metastatic and hormone-resistant phenotype. After an initial step of hyperplasia, in a high percentage of cases a neoplastic transformation event occurs that, less frequently, is followed by epithelial to mesenchymal...... cell lines compared to normal epithelial prostatic cells. Overexpression of miR-203 in brain or bone metastatic prostate cell lines (DU145 and PC3) is sufficient to induce a mesenchymal to epithelial transition with inhibition of cell proliferation, migration and invasiveness. We have identified CKAP2...

  9. Diffusion of chlorine in single-crystal (Sr,Y)Cl2.03

    International Nuclear Information System (INIS)

    Goff, J.P.; Hayes, W.; Ward, R.C.C; Hull, S.; Hutchings, M.T.

    1992-01-01

    Quasielastic energy broadening of the incoherent neutron scattering from single-crystal (Sr,Y)Cl 2.03 has been studied at elevated temperatures using the time-of-flight spectrometer IRIS at the Rutherford-Appleton Laboratory. Incoherent spectra measured at temperatures of 923 and 973 K have been fitted by a Chudley-Elliott model, in which individual anions occupy sites for a mean residence time τ before hopping to adjacent regular lattice sites. These results obtained from an anion-excess system are compared with a previous investigation of chlorine diffusion in pure SrCl 2 . (orig.)

  10. Final report for tank 241-B-203, push mode cores 115, 120 and 122

    International Nuclear Information System (INIS)

    Jo, J.

    1996-01-01

    This is the final laboratory report for tank 241-B-203 (B-203), cores 115, 120 and 122. Two fourteen-segment and one eleven-segment push-mode core samples from tank B-203 and a field blank sample were received at the 222-S Laboratory. Cores 115 (11 segments) and 120 (14 segments) were obtained from riser 2 and core 122 (14 segments) was obtained from riser 7. Core 115 was archived due to poor sample recovery and not analyzed (with an exception of liner liquid). The other two core samples underwent safety screening analyses in accordance with the sampling and analysis plan, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), total alpha analysis, and bulk density measurements. Bromide analysis by ion chromatography (IC) and lithium analysis by inductively coupled plasma atomic emission spectroscopy (ICP) were performed to determine if the samples were contaminated with any lithium bromide solution that may have been used during sampling. Total organic carbon (TOC) and cyanide (CN) analyses were completed for two samples with high exotherms. In addition to the core sample analysis, the tank headspace flammability was measured prior to core sampling as required by the SAP and Safety Screening DQO. None of the data indicate that the tank is unsafe when compared to the criteria in the Safety Screening Data Quality Objective. The tank has a high moisture content (approximately 75%). Two samples exceeded the DSC notification limit. However, re-analysis of these samples could not reproduce the same results (no exotherms detected). Also, secondary TOC and CN analyses on these samples indicated negligible fuel content. The one-sided 95-percent confidence intervals for total alpha results are well below the notification limit. Furthermore, the vapor in the tank B-203 dome space is far below the 25% lower flammability limit (LFL) stated in the SAP. Therefore, the results show that this tank may be considered safe. Water with a lithium bromide tracer, was

  11. LncRNA GAS5 Represses Osteosarcoma Cells Growth and Metastasis via Sponging MiR-203a

    Directory of Open Access Journals (Sweden)

    Yang Wang

    2018-01-01

    Full Text Available Background/Aims: LncRNA GAS5, a growth suppressor, has been reported to exert anti-tumor actions in various cancers, whereas the exact mechanism underling the anti-tumor action is still unclear. This study was aimed to investigate the effect of lncRNA GAS5 on osteosarcoma and tried to decode the underling mechanisms. Methods: Expressions of lncRNA GAS5 in MG-63 cells were silenced by shRNA transfection, while were overexpressed by vector transfection. Cell viability, migration, invasion and apoptosis were respectively assessed by MTT, Transwell assay and flow cytometry. Regulations between lncRNA GAS5 and miR-203a, as well as between miR-203a and TIMP2 were detected by qPCR, western blot and dual luciferase activity assay. Results: LncRNA GAS5 was down-regulated in MG-63 and OS-732 cells compared to hFOB1.19 cells. Silence of lncRNA GAS5 significantly promoted MG-63 cells viability, migration and invasion, and up-regulated Cyclin D1, Cyclin B1, CDK1 and CDK4 expressions. miR-203a was negatively regulated by lncRNA GAS5. The promoting activities of lncRNA GAS5 silence on MG-63 cells growth and metastasis were reversed by miR-203a suppression. TIMP2 was a target of miR-203a and the anti-growth and anti-metastasis actions of miR-203a suppression were reversed by TIMP2 silence. Further, lncRNA GAS5 silence, miR-203a overexpression, and TIMP2 silence could activate PI3K/AKT/GSK3β signaling while block NF-κB signaling. Conclusion: LncRNA GAS5 might be a tumor suppressor in osteosarcoma via sponging miR-203a, sequestering miR-203a away from TIMP2.

  12. Fate and implications of /sup 203/Pb ingestion in a dairy cow and a calf

    Energy Technology Data Exchange (ETDEWEB)

    Potter, G D; McIntyre, D R; Vattuone, G M

    1971-01-01

    Experiments were performed to study the internal transport and biological availability of radioactive lead that could be produced and released in certain types of nuclear cratering events, especially lead 203 and lead 210. A lactating dairy cow was used for the metabolic experiments, and a calf was used for the tissue distribution study. Urine, feces, blood, and milk samples from the cow were analyzed for each 24 hour period. Blood plasma and representative organs and tissues were removed from the calf and analyzed. Results showed a total recovery of 94.768% of the /sup 203/Pb in the feces, urine and milk of the cow by 144 hr. Approximately 85% was recovered in the feces in the first 48 hr. The total secretion in urine during 144 hr. was 0.173%, while the total secretion in milk was 0.190. These results reveal the slow, incomplete absorption of lead from the digestive tract. In the calf the tissue distribution of the absorbed lead shows that the erythrocytes represents the largest pool of lead in respect to recovery value. The tissue data confirm the known pattern of distribution of inorganic lead in soft tissues, with the highest concentrations in the liver and kidneys.

  13. Analysis on screening results of 2 203 premature infants with retinopathy

    Directory of Open Access Journals (Sweden)

    Qian Wang

    2018-06-01

    Full Text Available AIM: To investigate the incidence and risk factors of retinopathy of prematurity(ROPin preterm infants. METHODS: The wide-field digital pediatric Retinal imaging system was used to screen 2 203 preterm infants with gestational age RESULTS: Totally 367 infants(621 eyeswere diagnosed as retinopathy among 2 203 premature infants and the incidence of ROP was 16.66%; 236 cases(399 eyesof ROP(26.61%were detected in 887 cases of premature infants in accord with screening standard of the Chinese Premature Retinopathy Screening Guidelines(2014, and 131 cases(222 eyesof ROP(9.95%was detected in 1 316 cases of premature infants outside the screening standard. In our research, the incidence of ROP was related with gestational age, birth weight, oxygen duration and mechanical ventilation. However, the relationship had not been found with artificial insemination, caesarean birth, gender, polyembryony, acute respiratory distress syndrome(ARDS, hypertensive disorders in pregnancy, gestational diabetes mellitus, intrauterine infection, intrauterine distress, premature rupture of membrane. The incidence of ROP was statistically significant between different gestational age groups, different birth weight groups and different oxygen groups(PCONCLUSION: The incidence of ROP is 16.66% in this study, and there is still a certain proportion outside the screening standard of the Chinese Premature Retinopathy Screening Guidelines(2014. Gestational age, birth weight, oxygen duration and mechanical ventilation are high risk factors for ROP.

  14. Gastric dilatation volvulus: a retrospective study of 203 dogs with ventral midline gastropexy.

    Science.gov (United States)

    Ullmann, B; Seehaus, N; Hungerbühler, S; Meyer-Lindenberg, A

    2016-01-01

    To evaluate the recurrence rate of gastric dilatation volvulus and the incidence of complications in subsequent coeliotomies following ventral midline gastropexy. The medical records of dogs treated for gastric dilatation volvulus by ventral midline gastropexy were retrospectively reviewed. Owners were contacted and invited to complete a questionnaire and to return to the clinic for ultrasonographic and radiographic follow-up. The questionnaire was completed by 203 owners 2 to 123 months postoperatively, 24 of whom attended the follow-up examination. Of the 203 dogs, 13 (6 · 4%) underwent subsequent ventral midline coeliotomy and none developed complications related to the gastropexy site. In 23 of the 24 re-evaluated dogs, the stomach was closely associated with the abdominal on radiography and/or ultrasound. The recurrence rate for clinical signs of gastric dilatation or gastric dilatation volvulus after ventral midline gastropexy was 6 · 4%. This study shows that the recurrence of gastric dilatation volvulus after ventral midline gastropexy is low and adhesion of the stomach to the abdominal wall is persistent in almost all dogs that were re-examined. The gastropexy site did not appear to interfere with subsequent coeliotomy. © 2015 British Small Animal Veterinary Association.

  15. The effects of thiamin on lead metabolism: organ distribution of lead 203.

    Science.gov (United States)

    Kim, J S; Hamilton, D L; Blakley, B R; Rousseaux, C G

    1992-01-01

    The effect of thiamin on the organ distribution of lead was evaluated in CD-1 mice exposed intragastrically or intraperitoneally to a single dose of lead acetate (100 micrograms) containing 100 microCi lead 203. They were treated with either thiamin (25 or 50 mg/kg body weight), calcium ethylenediaminetetraacetic acid (CaEDTA) (50 mg/kg body weight), or combinations of thiamin and CaEDTA. The whole body retention and the organ distribution of lead 203 varied depending upon the route of lead administration, dose of thiamin and the specific treatment combination. Thiamin (25 or 50 mg/kg) treatment increased the whole body retention of both intragastric and intraperitoneal lead by approximately 10% in each instance. Calcium ethylenediaminetetraacetic acid, either alone or in combination with thiamin (50 mg/kg) reduced the whole body retention of lead by as much as 14% regardless of route of lead exposure. The relative retention of lead by the liver, kidney and spleen was greater in mice exposed to lead by the intragastric route. Regardless of route, CaEDTA in the combined treatment reduced the relative retention of lead in both the liver and kidney, whereas thiamin alone only reduced the retention of lead in the kidney. The results of this study indicate that thiamin in combination with CaEDTA alters the distribution and retention of lead in a manner which may have therapeutic application as it relates to chelation therapy. PMID:1423063

  16. The anti-mycobacterial activity of the cytochrome bcc inhibitor Q203 can be enhanced by small-molecule inhibition of cytochrome bd.

    NARCIS (Netherlands)

    Lu, P.; Asseri, A.H.O.; Kremer, Martijn; Maaskant, Janneke; Ummels, Roy; Lill, H.; Bald, D.

    2018-01-01

    Mycobacterial energy metabolism currently attracts strong attention as new target space for development of anti-tuberculosis drugs. The imidazopyridine Q203 targets the cytochrome bcc complex of the respiratory chain, a key component in energy metabolism. Q203 blocks growth of Mycobacterium

  17. 30 CFR 203.60 - Who may apply for royalty relief on a case-by-case basis in deep water in the Gulf of Mexico or...

    Science.gov (United States)

    2010-07-01

    ...-case basis in deep water in the Gulf of Mexico or offshore of Alaska? 203.60 Section 203.60 Mineral... basis in deep water in the Gulf of Mexico or offshore of Alaska? You may apply for royalty relief under... REDUCTION IN ROYALTY RATES OCS Oil, Gas, and Sulfur General Royalty Relief for Pre-Act Deep Water Leases and...

  18. 24 CFR 257.203 - Calculation of up-front and annual mortgage insurance premiums for H4H program mortgages.

    Science.gov (United States)

    2010-04-01

    ... mortgage insurance premiums for H4H program mortgages. 257.203 Section 257.203 Housing and Urban... mortgage insurance premiums for H4H program mortgages. (a) Applicable premiums. Any mortgage presented for... LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES HOPE FOR HOMEOWNERS PROGRAM...

  19. 24 CFR 203.18c - One-time or up-front mortgage insurance premium excluded from limitations on maximum mortgage...

    Science.gov (United States)

    2010-04-01

    ... insurance premium excluded from limitations on maximum mortgage amounts. 203.18c Section 203.18c Housing and...-front mortgage insurance premium excluded from limitations on maximum mortgage amounts. After... LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES SINGLE FAMILY MORTGAGE...

  20. London penetration depth and thermal fluctuations in the sulphur hydride 203 K superconductor

    Energy Technology Data Exchange (ETDEWEB)

    Talantsev, E.F.; Crump, W.P. [Robinson Research Institute, Victoria University of Wellington, Lower Hutt (New Zealand); Storey, J.G.; Tallon, J.L. [Robinson Research Institute, Victoria University of Wellington, Lower Hutt (New Zealand); MacDiarmid Institute for Advanced Materials and Nanotechnology, Lower Hutt (New Zealand)

    2017-03-15

    Recently, compressed H{sub 2}S has been shown to become superconducting at 203 K under a pressure of 155 GPa. One might expect fluctuations to dominate at such temperatures. Using the magnetisation critical current, we determine the ground-state London penetration depth, λ{sub 0} = 189 nm, and the superconducting energy gap, Δ{sub 0} = 27.8 meV, and find these parameters are similar to those of cuprate superconductors. We also determine the fluctuation temperature scale, T{sub fluc} = 1470 K, which shows that, unlike the cuprates, T{sub c} of the hydride is not limited by fluctuations. This is due to its three dimensionality and suggests the search for better superconductors should refocus on three-dimensional systems where the inevitable thermal fluctuations are less likely to reduce the observed T{sub c}. (copyright 2016 by WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  1. Proteus mirabilis RMS 203 as a new representative of the O13 Proteus serogroup.

    Science.gov (United States)

    Palusiak, Agata; Siwińska, Małgorzata; Zabłotni, Agnieszka

    2015-01-01

    The unique feature of some Proteus O-polysaccharides is occurrence of an amide of galacturonic acid with N(ε)-[(S/R)-1-Carboxyethyl]-L-lysine, GalA6(2S,8S/R-AlaLys). The results of the serological studies presented here, with reference to known O-antigens structures suggest that GalA6(2S,8S/R-AlaLys) or 2S,8R-AlaLys contribute to cross-reactions of O13 Proteus antisera, and Proteeae LPSs. It was also revealed that the Proteus mirabilis RMS 203 strain can be classified into the O13 serogroup, represented so far by two strains: Proteus mirabilis 26/57 and Proteus vulgaris 8344. The O13 LPS is a serologically important antigen with a fragment common to LPSs of different species in the Proteeae tribe.

  2. 7 CFR 330.203 - Action on applications for permits to move plant pests; form of and conditions in permits.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Action on applications for permits to move plant pests... PEST REGULATIONS; GENERAL; PLANT PESTS; SOIL, STONE, AND QUARRY PRODUCTS; GARBAGE Movement of Plant Pests § 330.203 Action on applications for permits to move plant pests; form of and conditions in...

  3. Study of the distribution of methyl mercury (203Hg) by whole body autoradiography of macaque monkeys (Macaca irus)

    International Nuclear Information System (INIS)

    Benard, Patrick; Burgat-Sacaze, Viviane; Rico, Andre; Braun, J.-P.; Eghbali, Behrokh

    1978-01-01

    The distribution of methyl-mercury labelled with mercury-203 has been studied in Monkeys by whole body autoradiography technique. The mercury is rapidly absorbed. It is localized in all the body and mainly in well defined areas of the central nervous-system [fr

  4. Activation analysis of thallium in urine using the 203Tl(n,2n) 202Tl reaction

    International Nuclear Information System (INIS)

    Korob, R.O.; Cohen, I.M.; Lage, M.; Baro, G.B.

    1976-04-01

    The method developed by the authors of thallium determination in human urine, based on the 203 Tl(n,2n) 202 Tl reaction followed by chemical separation and measurement of the produced 202 Tl by gamma spectrometry, is described. Its application in some cases of intoxication by thallium is reported. The advantages and limitations of the described technique are discussed. (author) [es

  5. The SO-20.3 MC and A modernization plan final report

    International Nuclear Information System (INIS)

    Longmire, V.L.; Ensslin, Norbert; Files, C.; Joseph, Joshua A. Jr.; Rudy, C.R.; Smith, M.K.; Russo, P.A.; Stevens, R.S.; Strittmatter, R.B.; Wilkey, D.D.; Pickett, C.; Brosey, W.; Swanson, J.

    2004-01-01

    Materials Control and Accountability (MC and A) provides assurance to the nation that nuclear materials are controlled in accordance with their strategic and economic importance and that the misuse, theft or diversion of these materials will be detected. MC and A plays an important part in security at nuclear facilities, especially in addressing threats such as theft of materials, environmental contamination, and nuclear safety incidents associated with nuclear materials. For this reason, it is important that MC and A takes advantage of new technologies and methods in order to provide information on a site's nuclear materials in the most timely and useful manner possible. Within the U.S. Department of Energy (DOE), the Office of Security Policy, Policy Integration and Technical Support Program (SO-20.3) is responsible for the development of safeguards and security technology that enables DOE and NNSA facilities to safeguard their Special Nuclear Materials. The SO-20.3 Program tasked safeguards personnel at Los Alamos National Laboratory to lead a project with representatives from the Y-12 Plant, Lawrence Livermore National Laboratory, and the Savannah River Site to prepare an MC and A Modernization Plan that provides recommendations for development new technologies and methodologies for MC and A in both new and existing DOE facilities. The team was tasked with taking into account new concerns about the protection of nuclear material following the attacks of September 11, 2001. Opportunities for applying new MC and A approaches and technologies that provide increased freedom of operation, increased security and provide a potential for cost savings in existing and new DOE facilities are discussed in this report.

  6. Biodistribution and radiation dosimetry of a positron emission tomographic ligand, 18F-SP203, to image metabotropic glutamate subtype 5 receptors in humans

    International Nuclear Information System (INIS)

    Kimura, Yasuyuki; Simeon, Fabrice G.; Pike, Victor W.; Innis, Robert B.; Fujita, Masahiro; Hatazawa, Jun; Mozley, P.D.

    2010-01-01

    A new PET ligand, 3-fluoro-5-(2-(2- 18 F-(fluoromethyl)-thiazol-4-yl)ethynyl)benzonitrile ( 18 F-SP203), is a positron emission tomographic radioligand selective for metabotropic glutamate subtype 5 receptors. The purposes of this study were to estimate the radiation-absorbed doses of 18 F-SP203 in humans and to determine from the distribution of radioactivity in bone structures with various proportions of bone and red marrow whether 18 F-SP203 undergoes defluorination. Whole-body images were acquired for 5 h after injecting 18 F-SP203 in seven healthy humans. Urine was collected at various time points. Radiation-absorbed doses were estimated by the Medical Internal Radiation Dose scheme. After injecting 18 F-SP203, the two organs with highest radiation exposure were urinary bladder wall and gallbladder wall, consistent with both urinary and fecal excretion. In the skeleton, most of the radioactivity was in bone structures that contain red marrow and not in those without red marrow. Although the dose to red marrow (30.9 μSv/MBq) was unusually high, the effective dose (17.8 μSv/MBq) of 18 F-SP203 was typical of that of other 18 F radiotracers. 18 F-SP203 causes an effective dose in humans typical of several other 18 F radioligands and undergoes little defluorination. (orig.)

  7. Microautoradiographic study of the distribution of methylmercuric(/sup 203/) chloride in the Mallard duck (Anas platyrhynchos)

    Energy Technology Data Exchange (ETDEWEB)

    Pass, D A

    1975-01-01

    Following oral administration of CH/sub 3/ /sup 203/HgCl (an environmental pollutant) to adult Mallard ducks, a heavy concentration of labeled Hg was detected in the esophageal muscosa, liver and kidneys. The concentration of exposed Ag grains in other tissues was only slightly greater than that of the background. At a cellular level, a selective concentration of labeled Hg was seen only in the CNS, an accumulation being present in astrocytes in all regions of the brain and spinal cord and in ependymal cells. In 1 bird that received 0.684 mCi of /sup 203/Hg, a selective accumulation of Hg was present in the cytoplasm of brain and spinal cord neurons. The presence of CH/sub 3/HgCl in astrocytes may be related to functional changes in astrocytes.

  8. Maternal-fetal distribution of mercury ( sup 203 Hg) released from dental amalgam fillings

    Energy Technology Data Exchange (ETDEWEB)

    Vimy, M.J.; Takahashi, Y.; Lorscheider, F.L. (Univ. of Calgary, Alberta (Canada))

    1990-04-01

    In humans, the continuous release of Hg vapor from dental amalgam tooth restorations is markedly increased for prolonged periods after chewing. The present study establishes a time-course distribution for amalgam Hg in body tissues of adult and fetal sheep. Under general anesthesia, five pregnant ewes had twelve occlusal amalgam fillings containing radioactive 203Hg placed in teeth at 112 days gestation. Blood, amniotic fluid, feces, and urine specimens were collected at 1- to 3-day intervals for 16 days. From days 16-140 after amalgam placement (16-41 days for fetal lambs), tissue specimens were analyzed for radioactivity, and total Hg concentrations were calculated. Results demonstrate that Hg from dental amalgam will appear in maternal and fetal blood and amniotic fluid within 2 days after placement of amalgam tooth restorations. Excretion of some of this Hg will also commence within 2 days. All tissues examined displayed Hg accumulation. Highest concentrations of Hg from amalgam in the adult occurred in kidney and liver, whereas in the fetus the highest amalgam Hg concentrations appeared in liver and pituitary gland. The placenta progressively concentrated Hg as gestation advanced to term, and milk concentration of amalgam Hg postpartum provides a potential source of Hg exposure to the newborn. It is concluded that accumulation of amalgam Hg progresses in maternal and fetal tissues to a steady state with advancing gestation and is maintained. Dental amalgam usage as a tooth restorative material in pregnant women and children should be reconsidered.

  9. Maternal-fetal distribution of mercury (203Hg) released from dental amalgam fillings

    International Nuclear Information System (INIS)

    Vimy, M.J.; Takahashi, Y.; Lorscheider, F.L.

    1990-01-01

    In humans, the continuous release of Hg vapor from dental amalgam tooth restorations is markedly increased for prolonged periods after chewing. The present study establishes a time-course distribution for amalgam Hg in body tissues of adult and fetal sheep. Under general anesthesia, five pregnant ewes had twelve occlusal amalgam fillings containing radioactive 203Hg placed in teeth at 112 days gestation. Blood, amniotic fluid, feces, and urine specimens were collected at 1- to 3-day intervals for 16 days. From days 16-140 after amalgam placement (16-41 days for fetal lambs), tissue specimens were analyzed for radioactivity, and total Hg concentrations were calculated. Results demonstrate that Hg from dental amalgam will appear in maternal and fetal blood and amniotic fluid within 2 days after placement of amalgam tooth restorations. Excretion of some of this Hg will also commence within 2 days. All tissues examined displayed Hg accumulation. Highest concentrations of Hg from amalgam in the adult occurred in kidney and liver, whereas in the fetus the highest amalgam Hg concentrations appeared in liver and pituitary gland. The placenta progressively concentrated Hg as gestation advanced to term, and milk concentration of amalgam Hg postpartum provides a potential source of Hg exposure to the newborn. It is concluded that accumulation of amalgam Hg progresses in maternal and fetal tissues to a steady state with advancing gestation and is maintained. Dental amalgam usage as a tooth restorative material in pregnant women and children should be reconsidered

  10. Optimization of procedures for mercury-203 instrumental neutron activation analysis in human urine

    Energy Technology Data Exchange (ETDEWEB)

    Blotcky, A J; Claassen, J P [Nebraska Univ., Omaha, NE (United States). Medical Center; Fung, Y K [Nebraska Univ., Lincoln, NE (United States). Dept. of Chemistry; Meade, A G; Rack, E P [Nebraska Univ., Lincoln, NE (United States)

    1995-08-01

    Mercury, a known neurotoxin, has been implicated in etiology and pathogenesis of such disease states as Alzheimer`s and Parkinson`s diseases. There is concern that the exposure to mercury vapor released from dental amalgam restorations is a potential health hazard. Measurement of mercury concentrations in blood or urine may be useful in diagnosis of mercury poisoning and in assessing the extent exposure. This study describes the optimization of pre-neutron activation analysis procedures such as sampling, selection of irradiation and counting vials and acid digestion in order to minimize mercury loss via volatilization and/or permeation through containers. Therefore, the determination of mercury can be complicated by these potential losses. In the optimized procedure 20mL of urine was spiked with three different concentrations of mercury, digested with concentrated nitric acid, and placed in polypropylene vials for irradiation and counting. Analysis was performed by subtracting the Se-75 photopeak contribution to the 279 keV Hg-203 photopeak and applying the method of standard additions. Urinary mercury concentrations in normal human subjects were determined to be of the order of 10ng/mL. (author). 22 refs., 1 fig., 5 tabs.

  11. Evolution of a laparoscopic liver resection program: an analysis of 203 cases.

    Science.gov (United States)

    Elshamy, Mohammed; Takahashi, Hideo; Akyuz, Muhammet; Yazici, Pinar; Yigitbas, Hakan; Hammad, Abdulrahman Y; Aucejo, Federico N; Quintini, Cristiano; Fung, John; Berber, Eren

    2017-10-01

    Techniques for laparoscopic liver resection (LLR) have been developed over the past two decades. The aim of this study is to analyze the outcomes and trends of LLR. 203 patients underwent LLR between 2006 and 2015. Trends in techniques and outcomes were assessed dividing the experience into 2 periods (before and after 2011). Tumor type was malignant in 62%, and R0 resection was achieved in 87.7%. Procedures included segmentectomy/wedge resection in 64.5%. Techniques included a purely laparoscopic approach in 59.1% and robotic 12.3%. Conversion to open surgery was necessary in 6.4% cases. Mean hospital stay was 3.7 ± 0.2 days. 90-day mortality was 0% and morbidity 20.2%. Pre-coagulation and the robot were used less often, while the performance of resections for posteriorly located tumors increased in the second versus the first period. This study confirms the safety and efficacy of LLR, while describing the evolution of a program regarding patient and technical selection. With building experience, the number of resections performed for posteriorly located tumors have increased, with less reliance on pre-coagulation and the robot.

  12. Optimization of procedures for mercury-203 instrumental neutron activation analysis in human urine

    International Nuclear Information System (INIS)

    Blotcky, A.J.; Claassen, J.P.

    1995-01-01

    Mercury, a known neurotoxin, has been implicated in etiology and pathogenesis of such disease states as Alzheimer's and Parkinson's diseases. There is concern that the exposure to mercury vapor released from dental amalgam restorations is a potential health hazard. Measurement of mercury concentrations in blood or urine may be useful in diagnosis of mercury poisoning and in assessing the extent exposure. This study describes the optimization of pre-neutron activation analysis procedures such as sampling, selection of irradiation and counting vials and acid digestion in order to minimize mercury loss via volatilization and/or permeation through containers. Therefore, the determination of mercury can be complicated by these potential losses. In the optimized procedure 20mL of urine was spiked with three different concentrations of mercury, digested with concentrated nitric acid, and placed in polypropylene vials for irradiation and counting. Analysis was performed by subtracting the Se-75 photopeak contribution to the 279 keV Hg-203 photopeak and applying the method of standard additions. Urinary mercury concentrations in normal human subjects were determined to be of the order of 10ng/mL. (author). 22 refs., 1 fig., 5 tabs

  13. Study and development recuperation of Thallium-203 from bombarded targets of this metal in production of Thallium-201 via addition of Thallium-201 radioisotope as a tracer

    International Nuclear Information System (INIS)

    Shirazi, B.

    2006-01-01

    Thallium-203 is the main material for 201 TlCl 3 radiopharmaceutical. It is very important to recover the remaining enriched Thallium-203 in every stage of the process to be reused in the production loop. In this research, the recuperation of Thallium-203 was studied with Dowex 50 WΧ8 ion exchange resin and Thallium-201 as a tracer. The results based on filling of ( 1 8 X 1.5) cm columns with this resin showed that the best flow rate of eluent is 2 ml/min. Also the most suitable volumes are 200 mL (HNO 3 0.05 M), 400 mL (solution of Thallium-203), 100 mL (H 2 O), 500 mL (Citric acid), 100 mL (H 2 0), 100 mL (EDTA 0.5 M and pH∼12.5). 60 mL (H 2 0), respectively

  14. 30 CFR 203.41 - If I have a qualified deep well or a qualified phase 1 ultra-deep well, what royalty relief would...

    Science.gov (United States)

    2010-07-01

    ... 200 meters but entirely less than 400 meters deep that: (1) Occurs before December 18, 2008; and (2... § 203.31(b) applies. In both situations, your lease must be partly or entirely in less than 200 meters...

  15. Hanford Tanks 241-C-203 and 241-C-204: Residual Waste Contaminant Release Model and Supporting Data

    Energy Technology Data Exchange (ETDEWEB)

    Deutsch, William J.; Krupka, Kenneth M.; Lindberg, Michael J.; Cantrell, Kirk J.; Brown, Christopher F.; Schaef, Herbert T.

    2004-10-28

    This report describes the development of release models for key contaminants that are present in residual sludge remaining after closure of Hanford Tanks 241-C-203 (C-203) and 241-C-204 (C-204). The release models were developed from data generated by laboratory characterization and testing of samples from these two tanks. Key results from this work are (1) future releases from the tanks of the primary contaminants of concern (99Tc and 238U) can be represented by relatively simple solubility relationships between infiltrating water and solid phases containing the contaminants; and (2) high percentages of technetium-99 in the sludges (20 wt% in C-203 and 75 wt% in C-204) are not readily water leachable, and, in fact, are very recalcitrant. This is similar to results found in related studies of sludges from Tank AY-102. These release models are being developed to support the tank closure risk assessments performed by CH2M HILL Hanford Group, Inc., for the U.S. Department of Energy.

  16. Hanford Tanks 241-C-203 and 241 C 204: Residual Waste Contaminant Release Model and Supporting Data

    Energy Technology Data Exchange (ETDEWEB)

    Deutsch, William J.; Krupka, Kenneth M.; Lindberg, Michael J.; Cantrell, Kirk J.; Brown, Christopher F.; Schaef, Herbert T.

    2007-05-23

    This report was revised in May 2007 to correct 90Sr values in Chapter 3. The changes were made on page 3.9, paragraph two and Table 3.10; page 3.16, last paragraph on the page; and Tables 3.21 and 3.31. The rest of the text remains unchanged from the original report issued in October 2004. This report describes the development of release models for key contaminants that are present in residual sludge remaining after closure of Hanford Tanks 241-C-203 (C-203) and 241-C-204 (C-204). The release models were developed from data generated by laboratory characterization and testing of samples from these two tanks. Key results from this work are (1) future releases from the tanks of the primary contaminants of concern (99Tc and 238U) can be represented by relatively simple solubility relationships between infiltrating water and solid phases containing the contaminants; and (2) high percentages of technetium-99 in the sludges (20 wt% in C-203 and 75 wt% in C-204) are not readily water leachable, and, in fact, are very recalcitrant. This is similar to results found in related studies of sludges from Tank AY-102. These release models are being developed to support the tank closure risk assessments performed by CH2M HILL Hanford Group, Inc., for the U.S. Department of Energy.

  17. Hanford Tanks 241-C-203 and 241-C-204: Residual Waste Contaminant Release Model and Supporting Data

    International Nuclear Information System (INIS)

    Deutsch, William J.; Krupka, Kenneth M.; Lindberg, Michael J.; Cantrell, Kirk J.; Brown, Christopher F.; Schaef, Herbert T.

    2004-01-01

    This report describes the development of release models for key contaminants that are present in residual sludge remaining after closure of Hanford Tanks 241-C-203 (C-203) and 241-C-204 (C-204). The release models were developed from data generated by laboratory characterization and testing of samples from these two tanks. Key results from this work are (1) future releases from the tanks of the primary contaminants of concern (99Tc and 238U) can be represented by relatively simple solubility relationships between infiltrating water and solid phases containing the contaminants; and (2) high percentages of technetium-99 in the sludges (20 wt% in C-203 and 75 wt% in C-204) are not readily water leachable, and, in fact, are very recalcitrant. This is similar to results found in related studies of sludges from Tank AY-102. These release models are being developed to support the tank closure risk assessments performed by CH2M HILL Hanford Group, Inc., for the U.S. Department of Energy

  18. 30 CFR 203.31 - If I have a qualified phase 2 or qualified phase 3 ultra-deep well, what royalty relief would...

    Science.gov (United States)

    2010-07-01

    ... water less than 400 meters deep (see § 203.30(a)), has no existing deep or ultra-deep wells and that the... depths partly or entirely less than 200 meters and has not previously produced from a deep well (§ 203.30... which is 16,000 feet TVD SS and your lease is located in water 100 meters deep. Then in 2008, you drill...

  19. Effects of some chelating agents on the uptake and distribution of 203Hg2+ in the brown trout (Salmo trutta): Studies on ethyl- and isopropylxanthate diethyl- and diisopropyldithiophosphate, dimethyl- and diethyldithiocarbamate and pyridinethione

    International Nuclear Information System (INIS)

    Borg, K.; Gottofrey, J.; Tjaelve, H.

    1988-01-01

    Brown trout, Salmo trutta, were exposed to water containing 0.1 μg/l 203 Hg 2+ , alone or with potassium ethylxanthate (PEX), sodium isopropylxanthate (SIX), sodium diethyldithiophosphate (SEP), sodium diisopropyldithiophosphate (SIP), sodium dimethyldithiocarbamate (SMC), sodium diethyldithiocarbamate (SEC) or sodium pyridinethione (SPyr), respectively. After 1 week the uptake and distribution of the 203 Hg 2+ in the fish were examined by gamma spectrometry, SIX, SIP, SMC, SEC and SPyr induced 2-3 times higher 203 Hg 2+ concentrations in most tissues in comparison with trout exposed to 203 Hg 2+ only. In the trout exposed to PEX slightly enhanced 203 Hg 2+ levels were found only in some tissues, and after exposure to SEP a few tissues showed decreased 203 Hg 2+ concentrations. Determinations of chloroform/water partition coefficients showed that lipophilic chelates are formed between all the examined substances and the 203 Hg 2+ . However, SIX, SIP, SMC, SEC and SPyr, which induced markedly increased tissue levels of the metal, formed 203 Hg 2+ complexes with higher lipophilicities than SEX and SEP. A facilitated penetration of the lipophilic 203 Hg 2+ complexes over the gill membranes may underly the increment in the tissue levels of the metal, and the relative lipophilicity of the complexes may be of importance for this effect. In some instances, as with SEP, the 203 Hg 2+ chelated in complexes with low lipophilicity may even be less able to acumulate in some tissues than the non-complexed metal. (orig.)

  20. Restorative proctocolectomy with ileal pouch-anal anastomosis in 203 patients: the Auckland experience.

    Science.gov (United States)

    Neilly, P; Neill, M E; Hill, G L

    1999-01-01

    Restorative proctocolectomy with ileal pouch-anal anastomosis (IPAA) has become an established operation for patients with ulcerative colitis and familial adenomatous polyposis (FAP). The results of a 15-year experience with IPAA are reported. Between September 1982 and June 1997, 203 patients had IPAA surgery. From a review of the charts, data were collected on the surgical procedure, the diagnosis and early and late complications. Pouch function was assessed by means of a postal questionnaire. Of the 201 patients (median age of 32 years; 89 women) with complete records, 122 had J pouches, 65 had W pouches and 14 S pouches were constructed. The pre-operative diagnosis in 88% was ulcerative colitis and in 10% it was FAP. During a median follow-up time of 6.1 years the diagnoses were changed for 8% of the patients; in 4% the diagnosis was changed to Crohn's disease. The overall mortality was 1.5% (early = 2, late = 1). The overall morbidity was 62% (early = 17%, late = 52%). The pouch was removed or was non-functional in 9%. All patients with a final diagnosis of Crohn's disease have had their pouch excised. The median stool frequency was 4.0 (range 1.3-8.7) during the day, and 0.7 (range 0-2.1) during the night. The fewer night-time stools (J = 1.0+/-0.6; W = 0.4+/-0.5 P requirement of the W-pouch patients for anti-diarrhoeals (P = 0.004) were offset by the need for two W-pouch patients to pass a catheter to empty their pouches. The type of patients who present for IPAA surgery and the outcomes observed in this series of Auckland patients are similar to those reported from major centres elsewhere.

  1. Brain, kidney and liver sup 203 Hg-methyl mercury uptake in the rat: Relationship to the neutral amino acid carrier

    Energy Technology Data Exchange (ETDEWEB)

    Aschner, M [Department of Pharmacology and Toxicology, and the Interdepartmental Neuroscience Training Program, Albany Medical College, Albany, NY (USA)

    1989-01-01

    To investigate the effect of L-neutral amino acids on tissue levels of methyl mercury in the adult animal, rats were infused into the external jugular vein with solutions containing (a) 0.05 mM {sup 203}Hg-MeHgCl and saline, (b) 0.05 mM {sup 203}Hg-MgHgCl-0.1 mM L-cysteine, (c) 0.05 mM {sup 203}Hg-MeHgCl-0.1 mM L-cysteine-0.1 mM L-methionine, (d) 0.05 mM {sup 203}Hg-MeHgCl-0.1 mM L-leucine, or (e) 0.05 mM {sup 203}Hg-MeHgCl-0.1 mM L-cysteine-0.1 mM L-leucine. Groups of animals were sacrificed at 3 min. 7 hr, and 96 hr. Brain, kidney, and liver {sup 203}Hg radioactivity was measured by means of gamma-scintillation spectrometry. Brain {sup 203}Hg concentrations L-cysteine treated animals were significantly higher compared with saline treated animals (P<0.05) at 3 min., 7 hr and 96 hr. The coinjection or coinfusion of methyl mercury with L-cysteine and L-methionine abolished the L-cysteine-mediated brain {sup 203}Hg uptake (P<0.05), at each sacrifice time. Kidney and liver {sup 203}Hg concentrations were not significantly different in any of the treatment groups compared with controls, irrespective of the sacrifice time. Furthermore, the percentage of diffusible {sup 203}Hg (non-protein bound) at each sacrifice time was not statistically different irrespective of the treatment assigned. These results suggest that methyl mercury L-cysteine conjugates in the plasma may share a common transport step with the L-neutral amino acid carrier transport system and indicate the presence in brain capillaries of a transport system capable of selectively mediating methyl mercury uptake across the capillary endothelial cell membrane. (author).

  2. Synthesis and characterization in monkey of [{sup 11}C]SP203 as a radioligand for imaging brain metabotropic glutamate 5 receptors

    Energy Technology Data Exchange (ETDEWEB)

    Simeon, Fabrice G.; Liow, Jeih-San; Zhang, Yi; Hong, Jinsoo; Gladding, Robert L.; Zoghbi, Sami S.; Innis, Robert B.; Pike, Victor W. [National Institutes of Health, Molecular Imaging Branch, National Institute of Mental Health, Bethesda, MD (United States)

    2012-12-15

    [{sup 18}F]SP203 (3-fluoro-5-(2-(2-([{sup 18}F]fluoromethyl)-thiazol-4-yl)ethynyl)benzonitrile) is an effective high-affinity and selective radioligand for imaging metabotropic 5 receptors (mGluR5) in human brain with PET. To provide a radioligand that may be used for more than one scanning session in the same subject in a single day, we set out to label SP203 with shorter-lived {sup 11}C (t{sub 1/2} = 20.4 min) and to characterize its behavior as a radioligand with PET in the monkey. Iodo and bromo precursors were obtained by cross-coupling 2-fluoromethyl-4-((trimethylsilyl)ethynyl)-1,3-thiazole with 3,5-diiodofluorobenzene and 3,5-dibromofluorobenzene, respectively. Treatment of either precursor with [{sup 11}C]cyanide ion rapidly gave [{sup 11}C]SP203, which was purified with high-performance liquid chromatography. PET was used to measure the uptake of radioactivity in brain regions after injecting [{sup 11}C]SP203 intravenously into rhesus monkeys at baseline and under conditions in which mGluR5 were blocked with 3-[(2-methyl-1,3-thiazol-4-yl)ethynyl]pyridine (MTEP). The emergence of radiometabolites in monkey blood in vitro and in vivo was assessed with radio-HPLC. The stability of [{sup 11}C]SP203 in human blood in vitro was also measured. The iodo precursor gave [{sup 11}C]SP203 in higher radiochemical yield (>98 %) than the bromo precursor (20-52 %). After intravenous administration of [{sup 11}C]SP203 into three rhesus monkeys, radioactivity peaked early in brain (average 12.5 min) with a regional distribution in rank order of expected mGluR5 density. Peak uptake was followed by a steady decline. No radioactivity accumulated in the skull. In monkeys pretreated with MTEP before [{sup 11}C]SP203 administration, radioactivity uptake in brain was again high but then declined more rapidly than in the baseline scan to a common low level. [{sup 11}C]SP203 was unstable in monkey blood in vitro and in vivo, and gave predominantly less lipophilic radiometabolites

  3. Dose dependent transfer of 203lead to milk and tissue uptake in suckling offspring studied in rats and mice

    International Nuclear Information System (INIS)

    Palminger Hallen, I.; Oskarsson, A.

    1993-01-01

    The dose-dependent transfer of 203 Pb to milk and uptake in suckling rats and mice during a three-day nursing period was studied. On day 14 of lactation, the dams were administered a single intravenous dose of lead, labelled with 203 Pb, in four or five doses from 0.0005 to 2.0 mg Pb/kg b.wt. There was a linear relationship between Pb levels in plasma and milk of both species. The Pb milk: plasma ratios at 24 hr after administration were 119 and 89 in mice and rats, respectively. At 72 hr the Pb milk: plasma ratio had decreased to 72 in mice and 35 in rats. The tissue levels of lead in the suckling rats and mice were also linearly correlated with lead concentration in milk at 72 hr, showing that milk could be used as an indicator of lead exposure to the suckling offspring. It is concluded that lead is transported into rat and mouse milk to a very high extent and the excretion into milk is more efficient in mice than in rats. On the other hand, rat pups had higher lead levels in tissues than mice pups, which might be due to a higher bioavailability and/or a lower excretion of lead in rat pups. Thus, lead in breast milk could be used as a biological indicator of lead exposure in the mother as well as in the suckling offspring. (au) (38 refs.)

  4. Hanford Tanks 241-C-202 and 241-C-203 Residual Waste Contaminant Release Models and Supporting Data

    Energy Technology Data Exchange (ETDEWEB)

    Deutsch, William J.; Krupka, Kenneth M.; Lindberg, Michael J.; Cantrell, Kirk J.; Brown, Christopher F.; Mattigod, Shas V.; Schaef, Herbert T.; Arey, Bruce W.

    2007-09-13

    As directed by Congress, the U. S. Department of Energy (DOE) established the Office of River Protection in 1998 to manage DOE's largest, most complex environmental cleanup project – retrieval of radioactive waste from Hanford tanks for treatment and eventual disposal. Sixty percent by volume of the nation's high-level radioactive waste is stored at Hanford in aging deteriorating tanks. If not cleaned up, this waste is a threat to the Columbia River and the Pacific Northwest. CH2M Hill Hanford Group, Inc., is the Office of River Protection's prime contractor responsible for the storage, retrieval, and disposal of Hanford's tank waste. As part of this effort, CH2M HILL Hanford Group, Inc. contracted with Pacific Northwest National Laboratory (PNNL) to develop release models for key contaminants that are present in residual sludge remaining after closure of Hanford Tanks 241-C-203 (C-203) and 241-C-204 (C-204). The release models were developed from data generated by laboratory characterization and testing of samples from these two tanks. These release models are being developed to support the tank closure risk assessments performed by CH2M HILL Hanford Group, Inc., for DOE.

  5. High current Tl-203, Rh-103 targets preparation for cyclotron production of Tl-201 and Pd-103 radionuclides

    International Nuclear Information System (INIS)

    Arzumanov, A.; Berger, V.; Borissenko, A.; Gorodisskaya, N.; Ilmatov, I.; Knyazev, A.; Koptev, V.; Lyssukhin, S.; Platov, A.; Sychikov, G.; Zheltov, D.

    2004-01-01

    The objectives of the present work are to increase thermal stability of cyclotron targets for production of Tl-201 isotope, increase Tl-203 regeneration rate at radiochemical reprocessing of the targets and develop production technology for radiochemical sources based on Rd-103 isotope. Electrochemical coating of copper substrate with Tl increased the beam current at target irradiation from 100 μA to 125 μA. Further increase of the beam current results in sharp decrease of target stability time at irradiation to 15 min at beam current 150 μA. Thermal calculations and tests at the electron-beam stand predict satisfactory stability at such currents. The discrepancy with the irradiation results has not been explained. More accurate specification of regimes for Tl-203 electrochemical recovery from irradiated targets and better matching of the electrolyte composition made it possible to increase the recovery rate up to 99.5%. Before the present Project, the INP had no experience in production of radioactive sources based on Pd-103. Thermo-diffusion extraction of Pd-103 from irradiated rhodium foil has been chosen as a technology-defining method. The process assures good extraction rate and high purity of extracted isotope. Production of Pd-103 sources based on this technology is much simpler compared to the same based on electrochemical processes. (author)

  6. Investigation of parity mixing in 203Tl by measurement of the forward-backward asymmetry of γ-rays after β-decay

    International Nuclear Information System (INIS)

    Dydak, F.; Polaschegg, H.D.; Riehs, P.; Serentschy, G.; Weinzierl, P.

    1975-10-01

    A multiple detector arrangement of fixed geometry and high inherent accuracy has been used to measure the forward-backward assymmetry coefficient Asub(l), in the β-γ transition of 203 Hg→ 203 Tl. Moreover, a control value has been determined which should exhibit no asymmetry. A non-zero coefficient Asub(l) is indicative of small admixtures of opposite parity in nuclear states. The properties of a multiple detector arrangement and systematic asymmetries are discussed in detail. Sources of 203 Hg of high quality have been prepared with a mass separator. To avoid a systematic bias due to non-negligible quadratic terms in the statistical errors a new technique of data analysis has been applied. The measurements of several sources yielded for the asymmetry coefficient Asub(l)= -(2.6+-0.7) x 10 -4 and for the control value (1.3+-0.7) x 10 -4 , with quoted errors of one standard deviation

  7. Activation analysis of thallium by /sup 203/Tl(n,2n)/sup 202/Tl reaction in nuclear reactors

    Energy Technology Data Exchange (ETDEWEB)

    Cohen, I M; Resnizky, S M; Baro, G B [Comision Nacional de Energia Atomica, Buenos Aires (Argentina). Gerencia de Radioisotopos y Radiaciones

    1982-01-01

    Two techniques are described for neutron activation analysis of thallium in biological and geological materials, based on /sup 203/Tl(n,2n)/sup 202/Tl reaction in nuclear reactors. Co-precipitation with bismuth sulphide is used as a pre-concentration method of thallium in urine samples, and post-irradiation chemical separation is also performed. The detection limit is 0.5 ..mu..g/l, for 100 ml urine samples. For thallium determination in blendes a radiochemical separation is carried out after the irradiation. An extension of this technique is presented for glass and silicate rocks. Results for seven blende samples from different places of Argentina are shown. Analysis of two reference materials, the 614 glass from the National Bureau of Standards and the granodiorite GSP-1 from the U.S. Geological Survey were also performed. The results are in very good agreement with the certified or estimated values.

  8. Second harmonic generation of frequency-locked pulsed dye laser for selective photoionization of T1-203 isotope

    International Nuclear Information System (INIS)

    Lim, Gwon; Jeong, Do Young; Ko, Kwang Hoon; Kim, Jae Woo; Kim, Taek Soo; Rho, Sipyo; Kim, Cheol Jung

    2003-01-01

    We have constructed the frequency-locked pulsed dye laser system. It is composed with a GIM-type oscillator and 3 stage longitudinally pumped amplifiers. The pump laser is the second harmonic of pulse Nd:YAG laser at the repetition rate of 6 kHz. Frequency-locking of dye laser oscillator is actively controlled by the feedback loop between a photoionization signal of T1-203 isotope and a wavelength tuning control. The tuning mirror rotates the order of micro degree per a step of step motor. Feedback system for frequency locking is operated with a PC-based control interface, including the data analysis of photoionization signals and the wavelength control using step pumping method for a medical application. Therefor, the dye laser has to be locked at 583.66 nm for SHG or BBO crystal. With the frequency-locking system, the photoionization experiment has been done for more than 10 hours.

  9. Radiotoxicological properties of neohydrin, labelled with Hg/sup 197/,/sup 203/ in quantities used for diagnostic purposes

    Energy Technology Data Exchange (ETDEWEB)

    Karklinskaya, O N [Tsentral' nyj Inst. Usovershenstvovaniya Vrachej, Moscow (USSR)

    1975-07-01

    Experiments on rats showed that the chief place of accumulation of mercury isotopes is the kidney (specific activity in the kidneys was 25 to 30 times that in other organs). Doses due to labelled Neohydrin in kidney tissue, calculated by generally accepted formulas were: 20.1 rad for Neohydrin-Hg/sup 203/ and 0.08 rad for Neohydrin-Hg/sup 197/. Histological studies did not indicate any morphological changes in the kidneys. However, nonhomogeneous, focal granular dystrophy was noted, and in some places, vacuolar dystrophy. In the descending portion of the distal canals and the Henle lemniscus there was an expansion of the lumen, with places of heavy thickening of the epithelium. An unequal plethora of vessels, sometimes with small hemorrhages and of edema, was observed. Specific reactions to mercury were negative. Morphological studies did not show any blood changes.

  10. 75 FR 7942 - Airworthiness Directives; Airbus Model A310-203, -221, -222 Airplanes; and Model A300 F4-605R and...

    Science.gov (United States)

    2010-02-23

    ... Airworthiness Directives; Airbus Model A310-203, -221, -222 Airplanes; and Model A300 F4-605R and -622R...-222 airplanes, all serial numbers. (2) Airbus Model A300 F4-605R and A300 F4-622R airplanes, all...

  11. 13 CFR 123.203 - What interest rate will my business pay on a physical disaster business loan and what are the...

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What interest rate will my... Business Loans § 123.203 What interest rate will my business pay on a physical disaster business loan and what are the repayment terms? (a) SBA will announce interest rates with each disaster declaration. If...

  12. Japanese Language and Culture: 10-3Y, 20-3Y, 30-3Y. 3-Year Program Guide to Implementation

    Science.gov (United States)

    Alberta Education, 2009

    2009-01-01

    This guide to implementation is intended to support the Japanese Language and Culture 10-3Y, 20-3Y, 30-3Y Program of Studies. It was developed primarily for teachers, yet it includes information that may be useful for administrators and other stakeholders in their efforts to plan for and implement the new Japanese program of studies. Familiarity…

  13. 45 CFR 2540.203 - When must I conduct a State criminal registry check and a National Sex Offender Public Web site...

    Science.gov (United States)

    2010-10-01

    ... check and a National Sex Offender Public Web site check on an individual in a covered position? 2540.203... National Sex Offender Public Web site check on an individual in a covered position? (a) The State criminal... enrolls in, or is hired by, your program on or after October 1, 2009. (b) The National Sex Offender Public...

  14. 5 CFR 297.203 - Access by the parent of a minor or by the legal guardian of an individual declared to be...

    Science.gov (United States)

    2010-01-01

    ... Office. (c) Minors are not precluded from exercising personally those rights provided them by the Privacy... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Access by the parent of a minor or by the... Request for Access § 297.203 Access by the parent of a minor or by the legal guardian of an individual...

  15. Factors Affecting Examination Attrition: Does Academic Support Help? A Survey of ACN203S (Cost Accounting and Control) Students at Unisa

    Science.gov (United States)

    Tladi, Lerato Sonia

    2013-01-01

    This study sought to determine the attributing and contributing factors to examination absence as well as whether the academic and social support available to students had a role to play in discouraging or reducing absence from examinations using results from a quantitative survey of ACN203S (Cost Accounting and Control) students who were admitted…

  16. 31 CFR 380.3 - What collateral may I pledge if I am a Treasury Tax and Loan depositary under 31 CFR part 203...

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false What collateral may I pledge if I am a Treasury Tax and Loan depositary under 31 CFR part 203, and what value will you assign to it? 380...) FISCAL SERVICE, DEPARTMENT OF THE TREASURY BUREAU OF THE PUBLIC DEBT COLLATERAL ACCEPTABILITY AND...

  17. 30 CFR 203.30 - Which leases are eligible for royalty relief as a result of drilling a phase 2 or phase 3 ultra...

    Science.gov (United States)

    2010-07-01

    ... 400 meters deep. (b) The lease has not produced gas or oil from a deep well or an ultra-deep well, except as provided in § 203.31(b). (c) If the lease is located entirely in more than 200 meters and entirely less than 400 meters of water, it must either: (1) Have been issued before November 28, 1995, and...

  18. 28 CFR Appendix to Part 55 - Jurisdictions Covered Under Sections 4(f)(4) and 203(c) of the Voting Rights Act of 1965, as Amended

    Science.gov (United States)

    2010-07-01

    ... REGARDING LANGUAGE MINORITY GROUPS Pt. 55, App. Appendix to Part 55—Jurisdictions Covered Under Sections 4(f...)(4) and 203(c) of the Voting Rights Act of 1965, as Amended [Applicable language minority group(s... (Chinese, Filipino, Japanese, Vietnamese), Spanish heritage. Merced County Spanish heritage Monterey County...

  19. MicroRNA (miR)-203 and miR-205 expression patterns identify subgroups of prognosis in cutaneous squamous cell carcinoma.

    Science.gov (United States)

    Cañueto, J; Cardeñoso-Álvarez, E; García-Hernández, J L; Galindo-Villardón, P; Vicente-Galindo, P; Vicente-Villardón, J L; Alonso-López, D; De Las Rivas, J; Valero, J; Moyano-Sanz, E; Fernández-López, E; Mao, J H; Castellanos-Martín, A; Román-Curto, C; Pérez-Losada, J

    2017-07-01

    Cutaneous squamous cell carcinoma (CSCC) is the second most widespread cancer in humans and its incidence is rising. These tumours can evolve as diseases of poor prognosis, and therefore it is important to identify new markers to better predict its clinical evolution. We aimed to identify the expression pattern of microRNAs (miRNAs or miRs) at different stages of skin cancer progression in a panel of murine skin cancer cell lines. Owing to the increasing importance of miRNAs in the pathogenesis of cancer, we considered the possibility that miRNAs could help to define the prognosis of CSCC and aimed to evaluate the potential use of miR-203 and miR-205 as biomarkers of prognosis in human tumours. Seventy-nine human primary CSCCs were collected at the University Hospital of Salamanca in Spain. We identified differential miRNA expression patterns at different stages of CSCC progression in a well-established panel of murine skin cancer cell lines, and then selected miR-205 and miR-203 to evaluate their association with the clinical prognosis and evolution of human CSCC. miR-205 was expressed in tumours with pathological features recognized as indicators of poor prognosis such as desmoplasia, perineural invasion and infiltrative growth pattern. miR-205 was mainly expressed in undifferentiated areas and in the invasion front, and was associated with both local recurrence and the development of general clinical events of poor evolution. miR-205 expression was an independent variable selected to predict events of poor clinical evolution using the multinomial logistic regression model described in this study. In contrast, miR-203 was mainly expressed in tumours exhibiting the characteristics associated with a good prognosis, was mainly present in well-differentiated zones, and rarely expressed in the invasion front. Therefore, the expression and associations of miR-205 and miR-203 were mostly mutually exclusive. Finally, using a logistic biplot we identified three clusters

  20. Measurement of basophil-activating capacity of grass pollen allergens, allergoids and hypoallergenic recombinant derivatives by flow cytometry using anti-CD203c.

    Science.gov (United States)

    Kahlert, H; Cromwell, O; Fiebig, H

    2003-09-01

    The assessment of the basophil-activating potential is an important aspect in the development of improved preparations for specific immunotherapy. The aim of the study was to evaluate the suitability of CD203c expression as a measure of basophil activation to compare allergoids with original allergen extracts, and recombinant hypoallergenic allergen derivatives with recombinant wild-type and natural allergens. Heparinized whole blood samples from grass pollen allergic subjects were stimulated with grass pollen allergens and allergen derivatives followed by labelling of the basophils with PE-conjugated anti-CD203c. After lysis of the erythrocytes and fixation, the basophils were detected by flow cytometry. In some experiments, histamine release was determined simultaneously. Grass pollen allergoids revealed a 10-10 000-fold reduction of basophil-activating capacity measured by CD203c expression. The deletion mutant DM4 of rPhl p 5b showed stronger hypoallergenic characteristics in a range of 50-10 000-fold reduction, whereas a combination mutant of rPhl p 5b and Phl p 6 revealed less hypoallergenic features. Histamine release experiments led to a similar outcome as CD203c measurement. The measurement of CD203c expression on basophils by flow cytometry provides a rapid and sensitive method for the estimation of the allergic or hypoallergenic features of allergen preparations. The results demonstrated the hypoallergenicity of grass pollen allergoids and of the rPhl p 5b variant DM4, which may be a candidate in future preparations for specific immunotherapy.

  1. Tank Vapor Characterization Project: Headspace vapor characterization of Hanford Waste Tank U-203, Results from samples collected on August 8, 1995

    International Nuclear Information System (INIS)

    Pool, K.H.; Clauss, T.W.; Evans, J.C.; McVeety, B.D.; Thomas, B.L.; Olsen, K.B.; Fruchter, J.S.; Ligotke, M.W.

    1995-11-01

    This report describes the analytical results of vapor samples taken from the headspace of the waste storage tank 241-U-203 (Tank U-203) at the Hanford Site in Washington State. The results described in this report were obtained to characterize the vapors present in the tank headspace and to support safety evaluations and tank-farm operations. The results include air concentrations of selected inorganic and organic analytes and grouped compounds from samples obtained by Westinghouse Hanford Company (WHC) and provided for analysis to Pacific Northwest Laboratory (PNL). Analyses were performed by the Vapor Analytical Laboratory (VAL) at PNL. Analyte concentrations were based on analytical results and, where appropriate, sample volumes provided by WHC. A summary of the results is listed. Detailed descriptions of the analytical results appear in the text

  2. Unusual effects of manual grinding and subsequent annealing process observed in Gd5.09Ge2.03Si1.88 compound

    Science.gov (United States)

    Carvalho, A. M. G.; Alves, C. S.; Trevizoli, P. V.; dos Santos, A. O.; Gama, S.; Coelho, A. A.

    2018-03-01

    The Gd5.09Ge2.03Si1.88 compound, as well as other magnetocaloric materials, certainly will not be used in their un-manufactured as-cast condition in future magnetic refrigeration applications or other devices. In this work, we have studied the Gd5.09Ge2.03Si1.88 compound processed in different ways, mainly, the as-cast powder, the annealed powder, and the pressed and sintered powder. The annealed powder (1370 K/20 h) does not present the monoclinic phase and the first-order magneto-structural transition observed in the as-cast powder. The pressed and sintered powder also do not present the first-order transition. Furthermore, the compacting pressure shifts the second-order magnetic transition to lower temperatures. The behavior of cell parameters as a function of the compacting pressure indicates that T C is directly affected by parameter c change.

  3. Ionic Liquid-salt based aqueous biphasic system for quick separation of no-carrier-added 203Pb from proton irradiated natTl2CO3 target

    International Nuclear Information System (INIS)

    Choudhury, Dibyasree; Ghosh, Kaustab; Lahiri, Susanta; Sarkar, Kangkana; Naskar, Nabanita

    2016-01-01

    In the present study, no-carrier-added (NCA) 203 Pb was radiochemically separated from bulk Tl target applying the ABS composing of 4 M K 2 HPO 4 and 0.1 mL of different strengths of IL (bmim)Cl ranging from 40% to 80% (w/v). Bulk Tl was spiked with 200Tl (T 1/2 = 26.1 h). A separation condition was achieved with 70% IL concentration and with 10 min shaking and 10 min settling time, where ∼84% of NCA 203 Pb was extracted into the IL rich phase with ∼10% contamination from bulk Tl. Therefore, further studies, i.e. variation in shaking and settling time were carried out keeping the concentration of IL fixed at 70%

  4. The L‐type Ca2+ channel facilitates abnormal metabolic activity in the cTnI‐G203S mouse model of hypertrophic cardiomyopathy

    Science.gov (United States)

    Viola, Helena; Johnstone, Victoria; Cserne Szappanos, Henrietta; Richman, Tara; Tsoutsman, Tatiana; Filipovska, Aleksandra; Semsarian, Christopher

    2016-01-01

    Key points Genetic mutations in cardiac troponin I (cTnI) are associated with development of hypertrophic cardiomyopathy characterized by myocyte remodelling, disorganization of cytoskeletal proteins and altered energy metabolism.The L‐type Ca2+ channel is the main route for calcium influx and is crucial to cardiac excitation and contraction. The channel also regulates mitochondrial function in the heart by a functional communication between the channel and mitochondria via the cytoskeletal network.We find that L‐type Ca2+ channel kinetics are altered in cTnI‐G203S cardiac myocytes and that activation of the channel causes a significantly greater increase in mitochondrial membrane potential and metabolic activity in cTnI‐G203S cardiac myocytes.These responses occur as a result of impaired communication between the L‐type Ca2+ channel and cytoskeletal protein F‐actin, involving decreased movement of actin–myosin and block of the mitochondrial voltage‐dependent anion channel, resulting in a ‘hypermetabolic’ mitochondrial state.We propose that L‐type Ca2+ channel antagonists, such as diltiazem, might be effective in reducing the cardiomyopathy by normalizing mitochondrial metabolic activity. Abstract Genetic mutations in cardiac troponin I (cTnI) account for 5% of families with hypertrophic cardiomyopathy. Hypertrophic cardiomyopathy is associated with disorganization of cytoskeletal proteins and altered energy metabolism. The L‐type Ca2+ channel (ICa‐L) plays an important role in regulating mitochondrial function. This involves a functional communication between the channel and mitochondria via the cytoskeletal network. We investigate the role of ICa‐L in regulating mitochondrial function in 25‐ to 30‐week‐old cardiomyopathic mice expressing the human disease‐causing mutation Gly203Ser in cTnI (cTnI‐G203S). The inactivation rate of ICa‐L is significantly faster in cTnI‐G203S myocytes [cTnI‐G203S: τ1 = 40.68 ± 3.22, n

  5. Renal Scintiscanning with Hg{sup 209}-Neohydrin in Urologic Diseases; Scintigraphy du Rein dans les Maladies des Voies Urin Aires au Moyen de Neohydrine Marquee avec {sup 203}Hg; Stsintillyatsionnoe skennirovanie pochek s pomoshch'yu neogidrina,mechennogo Rtut'yu-203, pri urologicheskikh zabolevaniyakh; Exploracion Centelleografica del Rinon con Neohydrina - {sup 203}hg en las Afecciones Urologicas

    Energy Technology Data Exchange (ETDEWEB)

    Bollini, V.; Tori, G. [Radiology Institute of the University of Bologna (Italy)

    1964-10-15

    Renal scintiscanning has been carried out using Hg{sup 203} - labelled Neohydrin. The equipment used is the Nuclear Chicago Isotope Scanner Type 1700 with 2 x 2 - i n crystal and a 7 - cm focal-length honeycomb collimator. Usually the scintigrams are registered both on paper and on photographic film. Hg{sup 203} - labelled Neohydrin is usually employed in doses of 120 - 160 {mu}c (about 2 {mu}c/kg). The scanning examination starts about 2 h after the injection and lasts about 40-50 min. The selective storage of labelled Neohydrin at the level of the renal cortex makes it possible to obtain a graphic representation of the two kidneys and to identify their position, orientation and shape, and the homogeneity of the actively functioning parenchyma. In some cases, autoradiographic investigations have been carried out on diseased kidneys after surgical removal. By this method anomalies in position and orientation, both congenital and acquired, can be ascertained. As regards shape, the limits of the renal patterns are the mote clearly defined as the renal function is better. Technical aspects such as the width of the crystal, type of collimator (whose focal plane should pass through the transverse medial plane of the kidneys), and dose - which should be not too high not too low - are essential conditions for a correct graphic reproduction of the renal patterns. Failure in tabular activity in the parenchyma is shown by a lack in the uptake of radioactivity. The position, extent and shape of such defects in uptake vary according to the entity of the renal structures and their derangement due to various pathological processes: tumours, cysts, hydronephrosis, tuberculosis etc. In normal subjects a homogeneous scintigram is usual, although a clearer hilar region is not a rare occurrence. Any circumscribed decrease of scintigraphic density outside the hilum is the expression of a less actively functioning zone. Among kidney patterns larger than normal, the homogeneous ones

  6. Comparative analysis of JKR Sarawak form of contract and Malaysia Standard form of building contract (PWD203A)

    Science.gov (United States)

    Yunus, A. I. A.; Muhammad, W. M. N. W.; Saaid, M. N. F.

    2018-04-01

    Standard form of contract is normally being used in Malaysia construction industry in establishing legal relation between contracting parties. Generally, most of Malaysia federal government construction project used PWD203A which is a standard form of contract to be used where Bills of Quantities Form Part of the Contract and it is issued by Public Works Department (PWD/JKR). On the other hand in Sarawak, the largest state in Malaysia, the state government has issued their own standard form of contract namely JKR Sarawak Form of Contract 2006. Even both forms have been used widely in construction industry; there is still lack of understanding on both forms. The aim of this paper is to identify significant provision on both forms of contract. Document analysis has been adopted in conducting an in-depth review on both forms. It is found that, both forms of contracts have differences and similarities on several provisions specifically matters to definitions and general; execution of the works; payments, completion and final account; and delay, dispute resolution and determination.

  7. Experiences with explicit finite-difference schemes for complex fluid dynamics problems on STAR-100 and CYBER-203 computers

    Science.gov (United States)

    Kumar, A.; Rudy, D. H.; Drummond, J. P.; Harris, J. E.

    1982-01-01

    Several two- and three-dimensional external and internal flow problems solved on the STAR-100 and CYBER-203 vector processing computers are described. The flow field was described by the full Navier-Stokes equations which were then solved by explicit finite-difference algorithms. Problem results and computer system requirements are presented. Program organization and data base structure for three-dimensional computer codes which will eliminate or improve on page faulting, are discussed. Storage requirements for three-dimensional codes are reduced by calculating transformation metric data in each step. As a result, in-core grid points were increased in number by 50% to 150,000, with a 10% execution time increase. An assessment of current and future machine requirements shows that even on the CYBER-205 computer only a few problems can be solved realistically. Estimates reveal that the present situation is more storage limited than compute rate limited, but advancements in both storage and speed are essential to realistically calculate three-dimensional flow.

  8. Acute avulsion fractures of the pelvis in adolescent competitive athletes: prevalence, location and sports distribution of 203 cases collected

    Energy Technology Data Exchange (ETDEWEB)

    Rossi, F.; Dragoni, S. [Sports Science Inst., Rome (Italy)

    2001-03-01

    Objective. To describe the prevalence, location and sports distribution of pelvic avulsion fractures in adolescent competitive athletes. Design and patients. One thousand two hundred and thirty-eight radiographs of the pelvis taken for focal traumatic symptoms in athletes with an age range of 11-35 years over a period of 22 years were reviewed. Results. One hundred and ninety-eight adolescent athletes were affected by 203 avulsion fractures of the pelvic apophyses (five cases presented multiple locations). The localisation was the ischial tuberosity (IT) in 109 cases, anterior inferior iliac spine (AIIS) in 45 cases, anterior superior iliac spine (ASIS) in 39 cases, superior corner of pubic symphysis (SCPS) in 7 cases and iliac crest (IC) in 3 cases. Soccer (74 cases) and gymnastics (55 cases) were the sports with the highest number of avulsion fractures documented. Conclusions. Apophyseal avulsion fractures of the pelvis in adolescent competitive athletes are most common in soccer and gymnastics. The lesions are usually the consequence of sudden and forceful muscle-tendon contractions during sport activities. Plain radiographs, are determinant for the diagnosis. (orig.)

  9. Uptake and clearance of mercury Hg (NO3)2-203Hg by the guppy (Lebistes reticulatus)

    International Nuclear Information System (INIS)

    Ferreira, J.R.; Rodrigues, N.S.; Nascimento Filho, V.F. do

    1982-01-01

    Fishes weighing between 20.6 and 536.9 mg were exposed to 25, 50 and 100 μg Hg.l -1 as Hg (NO 3 ) 2 labelled with 203 Hg, for 17 days in plastic aquaria containing 4 litre of soft aerated dechlorinated water. Whole body mercury contents were determined periodically by gamma counting, using a single channel spectrometer and a 3 in X 3 in NaI(Tl) well crystal. The highest bioconcentration of mercury was after 100 h of exposure for all the treatments. A further study on the clearance of mercury nitrate was carried out by transfering fishes to mercury-free water, after a period of 100 h of exposure in solutions of 35 and μg Hg.l -1 . The fishes were not sacrificed after the dose measurment, being returned to the aquaria for further sampling. The rate of clearance was similar for both concentrations. After 120 h exposure to uncontamined water, the fishes excreted the mercury previously absorbed. The correlation coefficient of the experimentals equations were 0.91 and 0.94, respectively, for the treatments 35 and 70 μg Hg.l -1 . Both experiments, uptake and clearance were made at same values of the water physico-chemical parameters (hardness = 36 mg CaCO 3 ; pH=7.2; O.D.=7.0 mg/l; temperature=23 +- 1 0 C). (Author) [pt

  10. Acute avulsion fractures of the pelvis in adolescent competitive athletes: prevalence, location and sports distribution of 203 cases collected

    International Nuclear Information System (INIS)

    Rossi, F.; Dragoni, S.

    2001-01-01

    Objective. To describe the prevalence, location and sports distribution of pelvic avulsion fractures in adolescent competitive athletes. Design and patients. One thousand two hundred and thirty-eight radiographs of the pelvis taken for focal traumatic symptoms in athletes with an age range of 11-35 years over a period of 22 years were reviewed. Results. One hundred and ninety-eight adolescent athletes were affected by 203 avulsion fractures of the pelvic apophyses (five cases presented multiple locations). The localisation was the ischial tuberosity (IT) in 109 cases, anterior inferior iliac spine (AIIS) in 45 cases, anterior superior iliac spine (ASIS) in 39 cases, superior corner of pubic symphysis (SCPS) in 7 cases and iliac crest (IC) in 3 cases. Soccer (74 cases) and gymnastics (55 cases) were the sports with the highest number of avulsion fractures documented. Conclusions. Apophyseal avulsion fractures of the pelvis in adolescent competitive athletes are most common in soccer and gymnastics. The lesions are usually the consequence of sudden and forceful muscle-tendon contractions during sport activities. Plain radiographs, are determinant for the diagnosis. (orig.)

  11. Investigation of the gamma radiation from the inelastic scattering of 2.75 MeV on 203Tl and 205Tl

    International Nuclear Information System (INIS)

    Hegewisch, S.

    1976-01-01

    The experimental presupositions for the measurements of (n,n'γ) reactions with time of flight discrimination have been established. By an investigation of the isotopes 203 Tl and 205 Tl with this method 120 gamma-transitions could be determined. The exact evaluation of their energies and intensities led to a completion of the level diagrams. The enrgies could be determined with a precision nearly one order of magnitude higher than the values existing hither to. (orig./WL) [de

  12. MiR-203 involves in neuropathic pain development and represses Rap1a expression in nerve growth factor differentiated neuronal PC12 cells.

    Science.gov (United States)

    Li, Haixia; Huang, Yuguang; Ma, Chao; Yu, Xuerong; Zhang, Zhiyong; Shen, Le

    2015-01-01

    Although microRNAs (miRNAs) have been shown to play a role in numerous biological processes, their function in neuropathic pain is not clear. The rat bilateral sciatic nerve chronic constriction injury (bCCI) is an established model of neuropathic pain, so we examined miRNA expression and function in the spinal dorsal horn in bCCI rats. Microarray and real-time polymerase chain reaction were used to examine the expression of miRNA in nerve system of bCCI rats, and the targets of miRNA were predicted by bioinformatic approaches. The function of specific miRNA was estimated through the methods of gene engineering. This study revealed substantially (∼10-fold) decreased miR-203 expression in the spinal dorsal horns but not the dorsal root ganglions, hippocampus, or anterior cingulate cortexes of bCCI rats. Rap1a protein expression was upregulated in bCCI rat spinal dorsal horns. We further verified that miR-203 directly targeted the 3'-untranslated region of the rap1a gene, thereby decreasing rap1a protein expression in neuron-like cells. Rap1a has diverse neuronal functions and their perturbation is responsible for several mental disorders. For example, Rap1a/MEK/ERK is involved in peripheral sensitization. These data suggest a potential role for miR-203 in regulating neuropathic pain development, and Rap1a is a validated target gene in vitro. Results from our study and others indicate the possibility that Rap1a may be involved in pain. We hope that these results can provide support for future research into miR-203 in gene therapy for neuropathic pain.

  13. IER 203 CED-2 Report: LLNL Final Design for BERP Ball With a Composite Reflector of Thin Polyethylene Backed by Nickel

    Energy Technology Data Exchange (ETDEWEB)

    Percher, C. M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Heinrichs, D. P. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Kim, S. K. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2016-07-18

    This report documents the results of final design (CED-2) for IER 203, BERP Ball Composite Reflection, and focuses on critical configurations with a 4.5 kg α-phase plutonium sphere reflected by a combination of thin high-density polyethylene (HDPE) backed by a thick nickel reflector. The Lawrence Livermore National Laboratory’s (LLNL’s) Nuclear Criticality Safety Division, in support of fissile material operations, calculated surprisingly reactive configurations when a fissile core was surrounded by a thin, moderating reflector backed by a thick metal reflector. These composite reflector configurations were much more reactive than either of the single reflector materials separately. The calculated findings have resulted in a stricter-than-anticipated criticality control set, impacting programmatic work. IER 203 was requested in response to these seemingly anomalous calculations to see if the composite reflection effect could be shown experimentally. This report focuses on the Beryllium Reflected Plutonium (BERP) ball as a fissile material core reflected by polyethylene and nickel. A total of four critical configurations were designed as part of CED-2. Fabrication costs are estimated to be $98,500, largely due to the cost of the large nickel reflectors. The IER 203 experiments could reasonably be expected to begin in early FY2017.

  14. Final Report for grant entitled "Production of Astatine-211 for U.S. Investigators"

    Energy Technology Data Exchange (ETDEWEB)

    Wilbur, Daniel Scott

    2012-12-12

    Alpha-particle emitting radionuclides hold great promise in the therapy of cancer, but few alpha-emitters are available to investigators to evaluate. Of the alpha-emitters that have properties amenable for use in humans, 211At is of particular interest as it does not have alpha-emitting daughter radionuclides. Thus, there is a high interest in having a source of 211At for sale to investigators in the US. Production of 211At is accomplished on a cyclotron using an alpha-particle beam irradiation of bismuth metal. Unfortunately, there are few cyclotrons available that can produce an alpha particle beam for that production. The University of Washington has a cyclotron, one of three in the U.S., that is currently producing 211At. In the proposed studies, the things necessary for production and shipment of 211At to other investigators will be put into place at UW. Of major importance is the efficient production and isolation of 211At in a form that can be readily used by other investigators. In the studies, production of 211At on the UW cyclotron will be optimized by determining the best beam energy and the highest beam current to maximize 211At production. As it would be very difficult for most investigators to isolate the 211At from the irradiated target, the 211At-isolation process will be optimized and automated to more safely and efficiently obtain the 211At for shipment. Additional tasks to make the 211At available for distribution include obtaining appropriate shipping vials and containers, putting into place the requisite standard operating procedures for Radiation Safety compliance at the levels of 211At activity to be produced / shipped, and working with the Department of Energy, Isotope Development and Production for Research and Applications Program, to take orders, make shipments and be reimbursed for costs of production and shipment.

  15. Reaction of aromatic diazonium salts with carrier-free radioiodine and astatine, evidence for complex formation

    International Nuclear Information System (INIS)

    Meyer, G.J.; Roessler, K.; Stoecklin, G.

    1979-01-01

    Systematic studies of the astatodiazoniation reaction and a comparison with iododediazoniation under comparable conditions are reported. The yields for all astatohalobenzenes and -toluenes were nearly constant and unaffected by the nature of the diazonium compound, its isomeric form, and the number of isomers used at the same time. Only astatofluorobenzenes were obtained at higher yields. An electron-transfer mechanism is proposed for dediazoniation at these low halide concentration levels. At sufficient thermal excitation levels the electron transfer leads to the dissociation of nitrogen, while the phenyl and halogen radicals recombine. The isomer distribution found for some of the derivatives from dediazoniation may also be due to steric effects

  16. Astatine-211-labeled biotin conjugates resistant to biotinidase for use in pretargeted radioimmunotherapy

    International Nuclear Information System (INIS)

    Foulon, Catherine F.; Alston, Kevin L.; Zalutsky, Michael R.

    1998-01-01

    We report herein the preparation and biological evaluation of two radioastatinated biotin conjugates, (3-[ 211 At]astatobenzoyl)norbiotinamide and ((5-[ 211 At]astato-3-pyridinyl)carbonyl)norbiotinamide. Both conjugates were stable in the presence of human serum and cerebrospinal fluid as well as murine serum, indicating a resistance to degradation to biotinidase. The normal tissue clearance of (3-[ 211 At]astatobenzoyl)norbiotinamide and ((5-[ 211 At]astato-3-pyridinyl)carbonyl)norbiotinamide was rapid, as observed previously with their iodo analogues. Also reported are the first syntheses of N-succinimidyl 5-[ 211 At]astato-3-pyridinecarboxylate and 3-[ 211 At]astatoaniline, two reagents of potential utility for labeling proteins and peptides with 211 At

  17. Microdosimetry of astatine-211 and comparison with that of iodine-125

    International Nuclear Information System (INIS)

    Unak, T.

    2001-01-01

    211 At is an alpha and Auger emitter radionuclide and has been frequently used for labeling of different kind of chemical agents. 125 I is also known as an effective Auger emitter. The radionuclides which emit short range and high LET radiations such as alpha particles and Auger electrons have high radiotoxic effectiveness on the living systems. The microdosimetric data are suitable to clarify the real radiotoxic effectiveness and to get the detail of diagnostic and therapeutic application principles of these radionuclides. In this study, the energy and dose absorptions by cell nucleus from alpha particles and Auger electrons emitted by 211 At have been calculated using a Monte Carlo calculation program (code: UNMOC). For these calculations two different model corresponding to the cell nucleus have been used and the data obtained were compared with the data earlier obtained for 125 I. As a result, the radiotoxicity of 211 At is in the competition with 125 I. In the case of a specific agent labelled with 211 At or 125 I is incorporated into the cell or cell nucleus, but non-bound to DNA or not found very close to it, 211 At should considerably be much more radiotoxic than 125 I, but in the case of the labelled agent is bound to DNA or take a place very close to it, the radiotoxicity of 125 I should considerably be higher than 211 At. (author)

  18. Evaluation of Novel Wet Chemistry Separation and Purification Methods to Facilitate Automation of Astatine-211 Isolation

    International Nuclear Information System (INIS)

    Wilbur, Daniel Scott

    2016-01-01

    This research is a collaborative effort between the research groups of the PIs, Dr. D. Scott Wilbur in the Department of Radiation Oncology at the University of Washington (UW) and Matthew O'Hara at the Pacific Northwest National Laboratory (PNNL). In this report only those studies conducted at UW and the budget information from UW will be reported. A separate progress and financial report will be provided by PNNL. This final report outlines the experiments (Tasks) conducted and results obtained at UW from July 1, 2013 thru June 30, 2016 (2-year project with 1 year no-cost extension). The report divides the information on the experiments and results obtained into the 5 specific objectives of the research efforts and the Tasks within those objectives. This format is used so that it is easy to see what has been accomplished in each area. A brief summary of the major findings from the studies is provided below. Summary of Major Findings from Research/Training Activities at UW: Anion and cation exchange columns did not provide adequate 211 At capture and/or extraction results under conditions studied to warrant further evaluation; PEG-Merrifield resins containing mPEG350, mPEG750, mPEG2000 and mPEG5000 were synthesized and evaluated; All of the mPEG resins with different sized mPEG moieties conjugated gave similar 211 At capture (>95%) from 8M HCl solutions and release with conc. NH 4 OH (~50-80%), but very low quantities were released when NaOH was used as an eluent; Capture and release of 211 At when loading [ 211 At]astatate appeared to be similar to that of [ 211 At]astatide on PEG columns, but further studies need to be conducted to confirm that; Capture of 211 At on PEG columns was lower (e.g. 80-90%) from solutions of 8M HNO 3 , but higher capture rates (e.g. 99%) can be obtained when 10M HNO 3 is mixed with an equal quantity of 8M HCl; Addition of reductants to the 211 At solutions did not appear to change the percent capture, but may have an effect on the % extracted; There was some indication that the PEG-Merrifield resins could be saturated (perhaps with Bi) resulting in lower capture percentages, but more studies need to be done to confirm that; A target dissolution chamber, designed and built at PNNL, works well with syringe pumps so it can be used in an automated system; Preliminary semi-automated 211 At isolation studies have been conducted with full-scale target dissolution and 211 At isolation using a PEG column on the Hamilton automated system gave low overall recoveries, but HNO 3 was used (rather than HCl) for loading the 211 At and flow rates were not optimized; Results obtained using PEG columns are high enough to warrant further development on a fully automated system; Results obtained also indicate that additional studies are warranted to evaluate other types of columns for 211 At separation from bismuth, which allow use of HNO 3 /HCl mixtures for loading and NaOH for eluting 211 At. Such a column could greatly simplify the overall isolation process and make it easier to automate.

  19. The potential of 211Astatine for NIS-mediated radionuclide therapy in prostate cancer

    International Nuclear Information System (INIS)

    Willhauck, Michael J.; Sharif Samani, Bibi-Rana; Goeke, Burkhard; Wolf, Ingo; Senekowitsch-Schmidtke, Reingard; Stark, Hans-Juergen; Meyer, Geerd J.; Knapp, Wolfram H.; Morris, John C.; Spitzweg, Christine

    2008-01-01

    We reported recently the induction of selective iodide uptake in prostate cancer cells (LNCaP) by prostate-specific antigen (PSA) promoter-directed sodium iodide symporter (NIS) expression that allowed a significant therapeutic effect of 131 I. In the current study, we studied the potential of the high-energy alpha-emitter 211 At, also transported by NIS, as an alternative radionuclide after NIS gene transfer in tumors with limited therapeutic efficacy of 131 I due to rapid iodide efflux. We investigated uptake and therapeutic efficacy of 211 At in LNCaP cells stably expressing NIS under the control of the PSA promoter (NP-1) in vitro and in vivo. NP-1 cells concentrated 211 At in a perchlorate-sensitive manner, which allowed a dramatic therapeutic effect in vitro. After intrapertoneal injection of 211 At (1 MBq), NP-1 tumors accumulated approximately 16% ID/g 211 At (effective half-life 4.6 h), which resulted in a tumor-absorbed dose of 1,580 ± 345 mGy/MBq and a significant tumor volume reduction of up to 82 ± 19%, while control tumors continued their growth exponentially. A significant therapeutic effect of 211 At has been demonstrated in prostate cancer after PSA promoter-directed NIS gene transfer in vitro and in vivo suggesting a potential role for 211 At as an attractive alternative radioisotope for NIS-targeted radionuclide therapy, in particular in smaller tumors with limited radionuclide retention time. (orig.)

  20. Sci-Thur AM: YIS – 01: New technologies for astatine-211 targeted alpha therapy research

    Energy Technology Data Exchange (ETDEWEB)

    Crawford, Jason; Yang, Hua; Schaffer, Paul; Ruth, Thomas [University of Victoria, Victoria, BC (Canada); TRIUMF, Vancouver, BC (Canada)

    2016-08-15

    Purpose: The short-range, densely ionizing α-particles emitted by {sup 211}At (t{sub 1/2}=7.2h) are well suited for the treatment of diffuse microscopic disease, using cancer targeting biomolecules. {sup 211}At availability is limited by the rarity of α-cyclotrons required for standard production. Image-based dosimetry is also limited for {sup 211}At, which emits low intensity X-rays. Our goal was to leverage state-of-the-art infrastructure at TRIUMF to produce and evaluate two related isotopes, {sup 211}Rn (t{sub 1/2}=14.6h, 73% decay to {sup 211}At) as a generator for {sup 211}At, and {sup 209}At (t{sub 1/2}=5.4h, X-ray/gamma-ray emitter) as a novel 211At surrogate for preclinical imaging studies. Methods: Produced by spallation of uranium with 480 MeV protons, mass separated ion beams of short-lived francium isotopes were implanted into NaCl targets where {sup 211}Rn or {sup 209}At were produced by radioactive decay, in situ. {sup 211}Rn was transferred to dodecane from which {sup 211}At was efficiently extracted and evaluated for clinical applicability. High energy SPECT/CT was evaluated for measuring {sup 209}At activity distributions in mice and phantoms. Results: Our small scale {sup 211}Rn/{sup 211}At generator system provided high purity {sup 211}At samples. The methods are immediately scalable to the level of radioactivity required for in vivo experiments with {sup 211}At. {sup 209}At-based high energy SPECT imaging was determined suitable for pursuing image-based dosimetry in mouse tumour models. In the future, we will utilize quantitative {sup 209}At-SPECT for image-based dose calculations. Conclusion: These early studies provided a foundation for future endeavours with {sup 211}At-based α-therapy. Canada is now significantly closer to clinical targeted α-therapy of cancer.

  1. Targeted radionuclide therapy with astatine-211: Oxidative dehalogenation of astatobenzoate conjugates.

    Science.gov (United States)

    Teze, David; Sergentu, Dumitru-Claudiu; Kalichuk, Valentina; Barbet, Jacques; Deniaud, David; Galland, Nicolas; Maurice, Rémi; Montavon, Gilles

    2017-05-31

    211 At is a most promising radionuclide for targeted alpha therapy. However, its limited availability and poorly known basic chemistry hamper its use. Based on the analogy with iodine, labelling is performed via astatobenzoate conjugates, but in vivo deastatination occurs, particularly when the conjugates are internalized in cells. Actually, the chemical or biological mechanism responsible for deastatination is unknown. In this work, we show that the C-At "organometalloid" bond can be cleaved by oxidative dehalogenation induced by oxidants such as permanganates, peroxides or hydroxyl radicals. Quantum mechanical calculations demonstrate that astatobenzoates are more sensitive to oxidation than iodobenzoates, and the oxidative deastatination rate is estimated to be about 6 × 10 6 faster at 37 °C than the oxidative deiodination one. Therefore, we attribute the "internal" deastatination mechanism to oxidative dehalogenation in biological compartments, in particular lysosomes.

  2. Production of Astatine-211 at the Duke University Medical Center for its regional distribution

    Energy Technology Data Exchange (ETDEWEB)

    Zalutsky, Michael [Duke University Medical Center, Durham, NC (United States)

    2016-01-01

    Systemic targeted radiation therapy and radioimmunotherapy continue to be important tools in the treatment of certain cancers. Because of their high energy and short path length, alpha particle emitters such as 211At are more effective than either external beam x- ray or in vivo beta radiation in delivering potentially curative doses of radiation. The limited clinical trials that have been conducted to date have yielded encouraging responses in some patients, e.g., malignant brain tumors. In order to escalate the additional necessary research and development in radiochemistry, radiobiology and efficacy evaluation of alpha particle radiotherapeutics, it is universally agreed that access to an affordable, reliable supply of 211At is warranted. In conjunction with the Department of Energy's intent to enhance stable and radioactive isotope availability for research applications, it is the primary objective of this project to improve 211At production and purification capabilities at Duke so that this radionuclide can be supplied to researchers at other institutions throughout the US.The most widely used 211At production method involves the α,2n reaction on Bismuth using a cyclotron with beams ≤ 28 MeV. Yields can be enhanced with use of an internal target that allows for a higher alpha fluence plus efficient heat dissipation in the target. Both of these items are in place at Duke; however, in order to support production for multi-institutional use, irradiation campaigns in excess of 50 µAp and four hours duration will be needed. Further, post-irradiation processing equipment is lacking that will enable the distribution process. Financial support is sought for i) a shielded, ventilated processing/containment hood; ii) development of a post-irradiation target retrieval system; iii) fabrication of a 211At distillation and recovery module and iv) a performance review and, where needed, an enhancement of seven major subsystems that comprise the CS-30 Cyclotron. With these modifications in place, routine production of ≥200 mCi of At-211 should be readily achievable, given our methodological development of At-211 target preparation, internal target irradiation and dry distillation to recover the radionuclide.

  3. Use of interhalogen exchange for preparation of astatine-benzene and iodo-benzene

    International Nuclear Information System (INIS)

    Kolachkovski, A.; Khalkin, V.A.

    1976-01-01

    Experimental testing of interhalogen exchange between the solid and liquid phases has been carried out at 155 deg C with particular reference to a NaOH-Na 131 I-BrPh system. Iodine transition rate is dependent on the process duration and alkali amount. The relative amounts of 131 IPh resultant from the reaction of interhalogen exchange is evaluated by paper chromatography. The results obtained may be considered as those which provide experimental support for the assumed efficiency of the production of 131 IPh based on the reaction of interhalogen exchange to yield 70-80% 131 IPh

  4. Preparation and evaluation of an astatine-211-labeled sigma receptor ligand for alpha radionuclide therapy

    International Nuclear Information System (INIS)

    Ogawa, Kazuma; Mizuno, Yoshiaki; Washiyama, Kohshin; Shiba, Kazuhiro; Takahashi, Naruto; Kozaka, Takashi; Watanabe, Shigeki; Shinohara, Atsushi; Odani, Akira

    2015-01-01

    Introduction: Sigma receptors are overexpressed in a variety of human tumors, making them potential targets for radionuclide receptor therapy. We have previously synthesized and evaluated 131 I-labeled (+)-2-[4-(4-iodophenyl)piperidino]cyclohexanol [(+)-[ 131 I]pIV], which has a high affinity for sigma receptors. Therefore, (+)-[ 131 I]pIV significantly inhibited tumor cell proliferation in tumor-bearing mice. In the present study, we report the synthesis and the in vitro and in vivo characterization of (+)-[ 211 At]pAtV, an 211 At-labeled sigma receptor ligand, that has potential use in alpha-radionuclide receptor therapy. Methods: The radiolabeled sigma receptor ligand (+)-[ 211 At]pAtV was prepared using a standard halogenation reaction generating a 91% radiochemical yield with 98% purity after HPLC purification. The partition coefficient of (+)-[ 211 At]pAtV was measured. Cellular uptake experiments and in vivo biodistribution experiments were performed using a mixed solution of (+)-[ 211 At]pAtV and (+)-[ 125 I]pIV; the human prostate cancer cell line DU-145, which expresses high levels of the sigma receptors, and DU-145 tumor-bearing mice. Results: The lipophilicity of (+)-[ 211 At]pAtV was similar to that of (+)-[ 125 I]pIV. DU-145 cellular uptake and the biodistribution patterns in DU-145 tumor-bearing mice at 1 h post-injection were also similar between (+)-[ 211 At]pAtV and (+)-[ 125 I]pIV. Namely, (+)-[ 211 At]pAtV demonstrated high uptake and retention in tumor via binding to sigma receptors. Conclusion: These results indicate that (+)-[ 211 At]pAtV could function as an new agent for alpha-radionuclide receptor therapy.

  5. Preparation and evaluation of an astatine-211-labeled sigma receptor ligand for alpha radionuclide therapy.

    Science.gov (United States)

    Ogawa, Kazuma; Mizuno, Yoshiaki; Washiyama, Kohshin; Shiba, Kazuhiro; Takahashi, Naruto; Kozaka, Takashi; Watanabe, Shigeki; Shinohara, Atsushi; Odani, Akira

    2015-11-01

    Sigma receptors are overexpressed in a variety of human tumors, making them potential targets for radionuclide receptor therapy. We have previously synthesized and evaluated (131)I-labeled (+)-2-[4-(4-iodophenyl)piperidino]cyclohexanol [(+)-[(131)I]pIV], which has a high affinity for sigma receptors. Therefore, (+)-[(131)I]pIV significantly inhibited tumor cell proliferation in tumor-bearing mice. In the present study, we report the synthesis and the in vitro and in vivo characterization of (+)-[(211)At]pAtV, an (211)At-labeled sigma receptor ligand, that has potential use in alpha-radionuclide receptor therapy. The radiolabeled sigma receptor ligand (+)-[(211)At]pAtV was prepared using a standard halogenation reaction generating a 91% radiochemical yield with 98% purity after HPLC purification. The partition coefficient of (+)-[(211)At]pAtV was measured. Cellular uptake experiments and in vivo biodistribution experiments were performed using a mixed solution of (+)-[(211)At]pAtV and (+)-[(125)I]pIV; the human prostate cancer cell line DU-145, which expresses high levels of the sigma receptors, and DU-145 tumor-bearing mice. The lipophilicity of (+)-[(211)At]pAtV was similar to that of (+)-[(125)I]pIV. DU-145 cellular uptake and the biodistribution patterns in DU-145 tumor-bearing mice at 1h post-injection were also similar between (+)-[(211)At]pAtV and (+)-[(125)I]pIV. Namely, (+)-[(211)At]pAtV demonstrated high uptake and retention in tumor via binding to sigma receptors. These results indicate that (+)-[(211)At]pAtV could function as an new agent for alpha-radionuclide receptor therapy. Copyright © 2015 Elsevier Inc. All rights reserved.

  6. Long non-coding RNA FBXL19-AS1 plays oncogenic role in colorectal cancer by sponging miR-203

    International Nuclear Information System (INIS)

    Shen, Bo; Yuan, Yuan; Zhang, Yan; Yu, Shaorong; Peng, Wei; Huang, Xin'en; Feng, Jifeng

    2017-01-01

    Long non-coding RNAs (lncRNAs) have emerged as critical regulators of the progression of human cancers, including colorectal cancer (CRC). The study of genome-wide lncRNA expression patterns in metastatic CRC could provide novel mechanism underlying CRC carcinogenesis. In here, we determined the lncRNA expression profiles correlating to CRC with or without lymph node metastasis (LNM) based on microarray analysis. We found that 2439 lncRNAs and 1654 mRNAs were differentially expressed in metastatic CRC relative to primary CRC. Among these dysregulated lncRNAs, FBXL19-AS1 was the most significantly upregulated lncRNA in metastatic tumors. Functionally, knockdown of FBXL19-AS1 played tumor-suppressive effects by inhibiting cell proliferation, migration and invasion in vitro and tumor growth and metastasis in vivo. Overexpression of FBXL19-AS1 was markedly correlated with TNM stage and LNM in CRC. Bioinformatics analysis predicted that miR-203 was potentially targeted by FBXL19-AS1, which was confirmed by dual-luciferase reporter assay. Pearson's correlation analysis showed that miR-203 expression was negatively related to FBXL19-AS1 in tumor tissues. Finally, miR-203 inhibition abrogated the effect of FBXL19-AS1 knockdown on the proliferation and invasion of LoVo cells. Our results reveal the cancer-promoting effect of FBXL19-AS1, acting as a molecular sponge in negatively modulating miR-203, which might provide a new insight for understanding of CRC development. - Highlights: • LncRNA expression signature was different between metastatic and primary tumors. • Knockdown of FBXL19-AS1 inhibits proliferation, migration and invasion in vitro. • Knockdown of FBXL19-AS1 inhibits tumorigenesis and metastasis in vivo. • FBXL19-AS1 was upregulated in CRC tissues and related with lymph node metastasis. • FBXL19-AS1, acting as a molecular sponge in negatively regulating miR-203.

  7. Metal transfer to plants grown on a dredged sediment: use of radioactive isotope 203Hg and titanium

    International Nuclear Information System (INIS)

    Caille, Nathalie; Vauleon, Clotilde; Leyval, Corinne; Morel, Jean-Louis

    2005-01-01

    Improperly disposed of dredged sediments contaminated with metals may induce long-term leaching and an increase of metal concentrations in ground waters and vegetal cover plants. The objective of the study was to quantify the sediment-to-plant transfer of Cu, Pb, Hg and Zn with a particular focus on the pathway of Hg and to determine whether the establishment of vegetal cover modifies the metal availability. A pot experiment with rape (Brassica napus), cabbage (Brassica oleraccea) and red fescue (Festuca rubra) was set up using a sediment first spiked with the radioisotope 203 Hg. Zinc concentrations (197-543 mg kg -1 DM) in leaves were higher than Cu concentration (197-543 mg kg -1 DM), Pb concentration (2.3-2.6 mg kg -1 DM) and Hg concentration (0.9-1.7 mg kg -1 DM). Leaves-to-sediment ratios decreased as follows: Zn>Cu>Hg>Pb. According to Ti measurements, metal contamination by dry deposition was less than 1%. Mercury concentration in plant leaves was higher than European and French thresholds. Foliar absorption of volatile Hg was a major pathway for Hg contamination with a root absorption of Hg higher in rape than in cabbage and red fescue. Growth of each species increased Cu solubility. Zinc solubility was increased only in the presence of rape. The highest increase of Cu solubility was observed for red fescue whereas this species largely decreased Zn solubility. Dissolved organic carbon (DOC) measurements suggested that Cu solubilisation could result from organic matter or release of natural plant exudates. Dissolved inorganic carbon (DIC) measures suggested that the high Zn solubility in the presence of rape could originate from a generation of acidity in rape rhizosphere and a subsequent dissolution of calcium carbonates. Consequently, emission of volatile Hg from contaminated dredged sediments and also the potential increase of metal solubility by a vegetal cover of grass when used in phytostabilisation must be taken into account by decision makers

  8. Metal transfer to plants grown on a dredged sediment: use of radioactive isotope 203Hg and titanium.

    Science.gov (United States)

    Caille, Nathalie; Vauleon, Clotilde; Leyval, Corinne; Morel, Jean-Louis

    2005-04-01

    Improperly disposed of dredged sediments contaminated with metals may induce long-term leaching and an increase of metal concentrations in ground waters and vegetal cover plants. The objective of the study was to quantify the sediment-to-plant transfer of Cu, Pb, Hg and Zn with a particular focus on the pathway of Hg and to determine whether the establishment of vegetal cover modifies the metal availability. A pot experiment with rape (Brassica napus), cabbage (Brassica oleraccea) and red fescue (Festuca rubra) was set up using a sediment first spiked with the radioisotope 203Hg. Zinc concentrations (197-543 mg kg(-1) DM) in leaves were higher than Cu concentration (197-543 mg kg(-1) DM), Pb concentration (2.3-2.6 mg kg(-1) DM) and Hg concentration (0.9-1.7 mg kg(-1) DM). Leaves-to-sediment ratios decreased as follows: Zn > Cu > Hg > Pb. According to Ti measurements, metal contamination by dry deposition was less than 1%. Mercury concentration in plant leaves was higher than European and French thresholds. Foliar absorption of volatile Hg was a major pathway for Hg contamination with a root absorption of Hg higher in rape than in cabbage and red fescue. Growth of each species increased Cu solubility. Zinc solubility was increased only in the presence of rape. The highest increase of Cu solubility was observed for red fescue whereas this species largely decreased Zn solubility. Dissolved organic carbon (DOC) measurements suggested that Cu solubilisation could result from organic matter or release of natural plant exudates. Dissolved inorganic carbon (DIC) measures suggested that the high Zn solubility in the presence of rape could originate from a generation of acidity in rape rhizosphere and a subsequent dissolution of calcium carbonates. Consequently, emission of volatile Hg from contaminated dredged sediments and also the potential increase of metal solubility by a vegetal cover of grass when used in phytostabilisation must be taken into account by decision

  9. Downregulation of miRNA-30c and miR-203a is associated with hepatitis C virus core protein-induced epithelial–mesenchymal transition in normal hepatocytes and hepatocellular carcinoma cells

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Dongjing [Hepatobiliary and Enteric Surgery Research Center, Xiangya Hospital, Central South University, Changsha 410008 (China); Wu, Jilin, E-mail: 6296082@qq.com [Hepatobiliary and Enteric Surgery Research Center, Xiangya Hospital, Central South University, Changsha 410008 (China); Liu, Meizhou [Department of Medical Service, Shenzhen Second People' s Hospital, Shenzhen, Guangdong 518035 (China); Yin, Hui [Staff' s Hospital, Central South University, Changsha, Hunan 410078 (China); He, Jiantai [Hepatobiliary and Enteric Surgery Research Center, Xiangya Hospital, Central South University, Changsha 410008 (China); Zhang, Bo, E-mail: zhangbo8095@126.com [Department of Ultrasonography, Xiangya Hospital, Central South University, Changsha, Hunan 410008 (China)

    2015-09-04

    Hepatitis C virus (HCV) Core protein has been demonstrated to induce epithelial–mesenchymal transition (EMT) and is associated with cancer progression of hepatocellular carcinoma (HCC). However, how the Core protein regulates EMT is still unclear. In this study, HCV Core protein was overexpressed by an adenovirus. The protein levels of EMT markers were measured by Western blot. The xenograft animal model was established by inoculation of HepG2 cells. Results showed that ectopic expression of HCV core protein induced EMT in L02 hepatocytes and HepG2 tumor cells by upregulating vimentin, Sanl1, and Snal2 expression and downregulating E-cadherin expression. Moreover, Core protein downregulated miR-30c and miR-203a levels in L02 and HepG2 cells, but artificial expression of miR-30c and miR-203a reversed Core protein-induced EMT. Further analysis showed that ectopic expression of HCV core protein stimulated cell proliferation, inhibited apoptosis, and increased cell migration, whereas artificial expression of miR-30c and miR-203a significantly reversed the role of Core protein in these cell functions in L02 and HepG2 cells. In the HepG2 xenograft tumor models, artificial expression of miR-30c and miR-203a inhibited EMT and tumor growth. Moreover, L02 cells overexpressing Core protein can form tumors in nude mice. In HCC patients, HCV infection significantly shortened patients' survival time, and loss of miR-30c and miR-203 expression correlated with poor survival. In conclusion, HCV core protein downregulates miR-30c and miR-203a expression, which results in activation of EMT in normal hepatocytes and HCC tumor cells. The Core protein-activated-EMT is involved in the carcinogenesis and progression of HCC. Loss of miR-30c and miR-203a expression is a marker for the poor prognosis of HCC. - Highlights: • HCV core protein downregulates miR-30c and miR-203a expression. • Downregulation of miR-30c and miR-203a activates EMT. • Activated-EMT is involved in the

  10. Post-irradiation examination of the vipac fuel assemblies IFA-104 and IFA-203, irradiated in the Halden boiling water reactor

    International Nuclear Information System (INIS)

    Linde, A. van der; Lucas Luyckx, H.J.B.

    1976-11-01

    Two seven pin assemblies, IFA-104 and IFA-203, have been irradiated in the Halding Boiling Water Reactor. The Zircaloy clad pins contained vibrationally compacted, sharp edged UO 2 particles with smear densities of 84-88% theoretical density and a fuel stack length of about 1520 mm. The IFA-104 pins operated satisfactorily during approximately 240 effective full power days at an average linear heat rating of about 340 W/cm, and a peak rating of about 480 W/cm. Ingress of water via a pressure transducer into one of the IFA-203 pins necessitated the premature termination of the irradiation after approximately 30 effective full power days at an average rating of about 460 W/cm, and a peak rating of about 550 W/cm. One IFA-104 pin and two IFA-203 pins failed early. The primary cause of these failures has been sought in the oxidation reaction of the tantalum tube which protected the W/Re thermocouples with the surrounding UO 2 or with moisture in the fuel at temperatures in excess of approximately 1700degC. The fuel centre temperature and the internal gas pressure measurements during the beginning of lifetime and the results of the post-irradiation chemical and microscopic analysis on fuel pin cross-sections have been correlated with corresponding data calculated on a Gapcon-Thermal computer programme written for pellet fuel pins. Fairly good agreement could be achieved between the measured data on the vipac pins and the calculated data of a pellet pin. During the corrosion of the Zircaloy-2 cladding of the IFA-104 pins which were autoclaved in 400degC steam prior to the irradiation, relatively large amounts of H 2 entered the cladding from the D 2 O coolant side. With 0.66 mole% H 2 O in the D 2 O the calculated hydrogen uptake preference ratio was 33

  11. Metal transfer to plants grown on a dredged sediment: use of radioactive isotope {sup 203}Hg and titanium

    Energy Technology Data Exchange (ETDEWEB)

    Caille, Nathalie [ENSAIA-INRA/INPL, 2 avenue de la Foret de Haye, F-54505 Vandoeuvre-les-Nancy (France); CNRSSP, 930 boulevard Lahure, F-59505 Douai (France); Laboratoire des Interactions Microorganismes-Mineraux-Matiere organique dans les sols, CNRS, 17 rue Notre-Dame des Pauvres, F-54505 Vandoeuvre-les-Nancy (France); Vauleon, Clotilde [CNRSSP, 930 boulevard Lahure, F-59505 Douai (France); Leyval, Corinne [Laboratoire des Interactions Microorganismes-Mineraux-Matiere organique dans les sols, CNRS, 17 rue Notre-Dame des Pauvres, F-54505 Vandoeuvre-les-Nancy (France); Morel, Jean-Louis [ENSAIA-INRA/INPL, 2 avenue de la Foret de Haye, F-54505 Vandoeuvre-les-Nancy (France)]. E-mail: jean-louis.Morel@ensaia.inpl-nancy.fr

    2005-04-01

    Improperly disposed of dredged sediments contaminated with metals may induce long-term leaching and an increase of metal concentrations in ground waters and vegetal cover plants. The objective of the study was to quantify the sediment-to-plant transfer of Cu, Pb, Hg and Zn with a particular focus on the pathway of Hg and to determine whether the establishment of vegetal cover modifies the metal availability. A pot experiment with rape (Brassica napus), cabbage (Brassica oleraccea) and red fescue (Festuca rubra) was set up using a sediment first spiked with the radioisotope {sup 203}Hg. Zinc concentrations (197-543 mg kg{sup -1} DM) in leaves were higher than Cu concentration (197-543 mg kg{sup -1} DM), Pb concentration (2.3-2.6 mg kg{sup -1} DM) and Hg concentration (0.9-1.7 mg kg{sup -1} DM). Leaves-to-sediment ratios decreased as follows: Zn>Cu>Hg>Pb. According to Ti measurements, metal contamination by dry deposition was less than 1%. Mercury concentration in plant leaves was higher than European and French thresholds. Foliar absorption of volatile Hg was a major pathway for Hg contamination with a root absorption of Hg higher in rape than in cabbage and red fescue. Growth of each species increased Cu solubility. Zinc solubility was increased only in the presence of rape. The highest increase of Cu solubility was observed for red fescue whereas this species largely decreased Zn solubility. Dissolved organic carbon (DOC) measurements suggested that Cu solubilisation could result from organic matter or release of natural plant exudates. Dissolved inorganic carbon (DIC) measures suggested that the high Zn solubility in the presence of rape could originate from a generation of acidity in rape rhizosphere and a subsequent dissolution of calcium carbonates. Consequently, emission of volatile Hg from contaminated dredged sediments and also the potential increase of metal solubility by a vegetal cover of grass when used in phytostabilisation must be taken into account

  12. The Clinical Phenotype of Idiopathic Rapid Eye Movement Sleep Behavior Disorder at Presentation: A Study in 203 Consecutive Patients.

    Science.gov (United States)

    Fernández-Arcos, Ana; Iranzo, Alex; Serradell, Mónica; Gaig, Carles; Santamaria, Joan

    2016-01-01

    To describe the clinical phenotype of idiopathic rapid eye movement (REM) sleep behavior disorder (IRBD) at presentation in a sleep center. Clinical history review of 203 consecutive patients with IRBD identified between 1990 and 2014. IRBD was diagnosed by clinical history plus video-polysomnographic demonstration of REM sleep with increased electromyographic activity linked to abnormal behaviors. Patients were 80% men with median age at IRBD diagnosis of 68 y (range, 50-85 y). In addition to the already known clinical picture of IRBD, other important features were apparent: 44% of the patients were not aware of their dream-enactment behaviors and 70% reported good sleep quality. In most of these cases bed partners were essential to convince patients to seek medical help. In 11% IRBD was elicited only after specific questioning when patients consulted for other reasons. Seven percent did not recall unpleasant dreams. Leaving the bed occurred occasionally in 24% of subjects in whom dementia with Lewy bodies often developed eventually. For the correct diagnosis of IRBD, video-polysomnography had to be repeated in 16% because of insufficient REM sleep or electromyographic artifacts from coexistent apneas. Some subjects with comorbid obstructive sleep apnea reported partial improvement of RBD symptoms following continuous positive airway pressure therapy. Lack of therapy with clonazepam resulted in an increased risk of sleep related injuries. Synucleinopathy was frequently diagnosed, even in patients with mild severity or uncommon IRBD presentations (e.g., patients who reported sleeping well, onset triggered by a life event, nocturnal ambulation) indicating that the development of a neurodegenerative disease is independent of the clinical presentation of IRBD. We report the largest IRBD cohort observed in a single center to date and highlight frequent features that were not reported or not sufficiently emphasized in previous publications. Physicians should be aware of

  13. Post-irradiation examinations on the KNK II/1 fuel element NY-203 with 400 equivalent full-power days residence time and 10 % burnup

    International Nuclear Information System (INIS)

    Patzer, G.; Geier, F.

    1984-09-01

    The fuel assembly NY-203 has been irradiated in the first core of KNK II up to a burnup of about 10 % and a residence time of 400 equivalent full-power days. The assembly contained 211 fuel pins with 6.0 mm outer diameter and fuel pellets with the composition (U 0 .7Pu 0 .3)O 2 .00. The cladding material was the austenitic steel 1.4988 lg. Some selected pins were examined in the hot cells of the KfK Karlsruhe. The post-irradiation examinations did not reveal any critical design aspects [de

  14. A comparison of the neuroprotective efficacy of individual oxime (HI-6) and combinations of oximes (HI-6+trimedoxime, HI-6+K203) in soman-poisoned rats.

    Science.gov (United States)

    Kassa, Jiri; Karasova, Jana Zdarova; Tesarova, Sandra

    2011-07-01

    The ability of two combinations of oximes (HI-6+trimedoxime, HI-6+K203) to reduce soman-induced acute neurotoxic signs and symptoms was compared with the neuroprotective efficacy of the oxime HI-6 alone, using a functional observational battery. Soman-induced neurotoxicity and the neuroprotective effects of HI-6 alone and HI-6 combined with trimedoxime or K203 in rats poisoned with soman at a sublethal dose (90 μg/kg intramuscularly, i.m.; 80% of LD₅₀ value) were monitored by the functional observational battery at 24 hours following soman administration. The results indicate that both tested oxime mixtures combined with atropine were able to allow soman-poisoned rats to survive 24 hours following soman challenge, while 4 nontreated soman-poisoned rats and 1 soman-poisoned rat treated with oxime HI-6 alone combined with atropine died within 24 hours following soman poisoning. While the oxime HI-6 alone combined with atropine treatment was able to eliminate a few soman-induced neurotoxic signs and symptoms, both oxime mixtures showed higher neuroprotective efficacy in soman-poisoned rats. Especially, the combination of HI-6 with trimedoxime was able to eliminate most soman-induced neurotoxic signs and symptoms and markedly reduce acute neurotoxicity of soman in rats. Thus, both tested mixtures of oximes combined with atropine were able to increase the neuroprotective effectiveness of antidotal treatment of acute soman poisonings, compared to the individual oxime.

  15. MicroRNAs 142-3p, miR-155 and miR-203 Are Deregulated in Gastric MALT Lymphomas Compared to Chronic Gastritis.

    Science.gov (United States)

    Fernández, Concepción; Bellosillo, Beatriz; Ferraro, Mariana; Seoane, Agustín; Sánchez-González, Blanca; Pairet, Silvia; Pons, Aina; Barranco, Luis; Vela, María Carmen; Gimeno, Eva; Colomo, Lluís; Besses, Carles; Navarro, Alfons; Salar, Antonio

    2017-01-02

    Over the last years, our knowledge on pathogenesis of gastric MALT lymphoma has greatly improved, but its morphological diagnosis is still hampered by overlapping histological features with advanced chronic gastritis. MicroRNAs are deregulated in lymphomas, but their role and usefulness in gastric MALT lymphoma has not been extensively investigated. We analyzed the expression of 384 miRNAs using TaqMan microRNA assay in a training series of 10 gastric MALT lymphomas, 3 chronic gastritis and 2 reactive lymph nodes. Then, significantly deregulated miRNAs were individually assessed by real-time PCR in a validation series of 16 gastric MALT lymphomas and 12 chronic gastritis. Gastric MALT lymphoma is characterized by a specific miRNA expression profile. Among the differentially expressed miRNAs, a significant overexpression of miR-142-3p and miR-155 and down-regulation of miR-203 was observed in gastric MALT lymphoma when compared to chronic gastritis. miR-142-3p, miR-155 and miR-203 expression levels might be helpful biomarkers for the differential diagnosis between gastric MALT lymphomas and chronic gastritis. Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

  16. Novel "203" type of heterostructured MoS2-Fe3O4-C ternary nanohybrid: Synthesis, and enhanced microwave absorption properties

    Science.gov (United States)

    Yang, Erqi; Qi, Xiaosi; Xie, Ren; Bai, Zhongchen; Jiang, Yang; Qin, Shuijie; Zhong, Wei; Du, Youwei

    2018-06-01

    It is widely recognized that constructing multiple interface structures to enhance interface polarization is very good for the attenuation of electromagnetic (EM) wave. Here, a novel "203" type of heterostructured nanohybrid consisting of two-dimensional (2D) MoS2 nanosheets, zero-dimensional (0D) Fe3O4 nanoparticles and three-dimensional (3D) carbon layers was elaborately designed and successfully synthesized by a two-step method: Fe3O4 nanoparticles were deposited onto the surface of few-layer MoS2 nanosheets by a hydrothermal method, followed by the carbonation process by a chemical vapor deposition method. Compared to that of "20" type MoS2-Fe3O4, the as-prepared heterostructured "203" type MoS2-Fe3O4-C ternary nanohybrid exhibited remarkably enhanced EM and microwave absorption properties. And the minimum reflection loss (RL) value of the obtained MoS2-Fe3O4-C ternary nanohybrid could reach -53.03 dB at 14.4 GHz with a matching thickness of 7.86 mm. Moreover, the excellent EM wave absorption property of the as-prepared ternary nanohybrid was proved to be attributed to the quarter-wavelength matching model. Therefore, a simple and effective route was proposed to produce MoS2-based mixed-dimensional van der Waals heterostructure, which provided a new platform for the designing and production of high performance microwave absorption materials.

  17. Acetylcholinesterase Reactivators (HI-6, Obidoxime, Trimedoxime, K027, K075, K127, K203, K282: Structural Evaluation of Human Serum Albumin Binding and Absorption Kinetics

    Directory of Open Access Journals (Sweden)

    Filip Zemek

    2013-08-01

    Full Text Available Acetylcholinesterase (AChE reactivators (oximes are compounds predominantly targeting the active site of the enzyme. Toxic effects of organophosphates nerve agents (OPNAs are primarily related to their covalent binding to AChE and butyrylcholinesterase (BChE, critical detoxification enzymes in the blood and in the central nervous system (CNS. After exposure to OPNAs, accumulation of acetylcholine (ACh overstimulates receptors and blocks neuromuscular junction transmission resulting in CNS toxicity. Current efforts at treatments for OPNA exposure are focused on non-quaternary reactivators, monoisonitrosoacetone oximes (MINA, and diacylmonoxime reactivators (DAM. However, so far only quaternary oximes have been approved for use in cases of OPNA intoxication. Five acetylcholinesterase reactivator candidates (K027, K075, K127, K203, K282 are presented here, together with pharmacokinetic data (plasma concentration, human serum albumin binding potency. Pharmacokinetic curves based on intramuscular application of the tested compounds are given, with binding information and an evaluation of structural relationships. Human Serum Albumin (HSA binding studies have not yet been performed on any acetylcholinesterase reactivators, and correlations between structure, concentration curves and binding are vital for further development. HSA bindings of the tested compounds were 1% (HI-6, 7% (obidoxime, 6% (trimedoxime, and 5%, 10%, 4%, 15%, and 12% for K027, K075, K127, K203, and K282, respectively.

  18. Modulation of miR-203 and its regulators as a function of time during the development of 7, 12 dimethylbenz [a] anthracene induced mouse skin tumors in presence or absence of the antitumor agents

    International Nuclear Information System (INIS)

    Tiwari, Prakash; Gupta, Krishna P.

    2014-01-01

    We investigated the chemopreventive effects of naturally occurring compounds like butyric acid (BA), nicotinamide (NA) and calcium glucarate (CAG) individually or in combination in 7, 12-dimethylbenz [a] anthracene (DMBA) treated mouse skin at 4 and 16 weeks, the time before and after the tumor development. DMBA application did not show any skin tumors at 4 weeks but well defined tumors appeared at 16 weeks. BA, NA or CAG prevented the tumor development significantly but the protection was highly enhanced when all these compounds were given together. In order to see the molecular changes progressing with tumors, we showed the downregulation of tumor suppressor miR-203 at 16 weeks and upregulation of histone deacetylases (HDAC), DNA methyltransferase, promoter methylation of miR-203 at 4 or 16 weeks. Regulators of micro RNA biogenesis such as DICER1 and Ago2 were also deregulated by DMBA. Proto-oncogene c-myc and BMI1 were upregulated and tumor suppressor gene p16 was downregulated by DMBA as a function of time. Effects of BA, NA or CAG were more pronounced after 16 weeks as compared to 4 weeks in preventing the tumor development and altered gene expression. Concomitant administration of BA, NA and CAG tried to prevent these alterations more effectively than that of individual compound possibly by regulating miR-203 status through epigenetic or biogenetic modulations before and after the tumor development. Study provides a rationale for chemoprevention by combination of different compounds targeting miR-203. - Highlights: • DMBA modulates miR-203 and its regulator before and after the onset of tumors. • Suppression of miR-203 and p16 could be the result of gene promoter methylation. • BA, NA or CAG prevents the effects of DMBA. • Combination of BA, NA or CAG is more effective in preventing the DMBA modulations

  19. Folding model analysis of 58Ni + 64Ni elastic and inelastic scattering at Elab=203.8 and 219.2 MeV

    International Nuclear Information System (INIS)

    Ruiz, J.A.; Ferrero, J.L.; Bilwes, B.; Bilwes, R.

    1992-01-01

    Angular distributions of elastic scattering of 58 Ni by 64 Ni and inelastic scattering leading to the first 2 + state in 58 Ni or in 64 Ni were measured at E lab =203.8 and 219.2 MeV. The data were analyzed in the frame of the folding model. A renormalization N of the folding potential consistently lower than unity (N∼0.65) is needed to reproduce the elastic scattering data. Coupled-channel calculations including the main inelastic channels explain partly this discrepancy. They reproduce the elastic and inelastic scattering data with a renormalization of N∼0.8 at both energies. Interference between Coulomb and nuclear excitation is shown to play an important role in the repulsive character of the polarization potential. (author) 38 refs.; 6 figs.; 1 tab

  20. Physiological studies of environmental pollutants. Progress report, September 1, 1975--May 31, 1976. [/sup 210/Po, /sup 203/Pb, /sup 201/Tl, /sup 207/Bi, /sup 65/Zn

    Energy Technology Data Exchange (ETDEWEB)

    Lengemann, F W; Wentworth, R A

    1976-01-01

    In the past year we have looked at the transfer of some members of the actinide decay series into milk of goats. These were /sup 210/Po, /sup 203/Pb, /sup 201/Tl and /sup 207/Bi. All of these appeared in milk after oral ingestion but at levels less than 1 percent per liter. In addition we have looked at the transfer of /sup 65/Zn into milk of goats after oral and I.V. doses; the experiments are incomplete at this time. In controlled temperature studies it was found that 6.6 times as much radioiodine was secreted into milk when goats were at 33/sup 0/ as opposed to 5/sup 0/C. When radioiodine is put into the mammary gland the transfer from milk to body is rapid; more rapid than is the case for /sup 65/Zn. The analysis of these data indicate the need for a model capable of handling expansion of a compartment.

  1. Tank characterization report for single-shell tanks 241-T-201, 241-T-202, 241-T-203, and 241-T-204

    International Nuclear Information System (INIS)

    Simpson, B.C.

    1998-01-01

    A major function of the Tank Waste Remediation System (TWRS) is to characterize waste in support of waste management and disposal activities at the Hanford Site. Analytical data from sampling and analysis, in addition to other available information about a tank are compiled and maintained in a tank characterization report (TCR). This report and its appendices serve as the TCR for the single-shell tank series consisting of 241-T-201, -T-202, -T-203, and -T-204. The objectives of this report are: (1) to use characterization data in response to technical issues associated with T-200 series tank waste and (2) to provide a standard characterization of this waste in terms of a best-basis inventory estimate. Section 2.0 summarizes the response to technical issues, Section 3.0 shows the best-basis inventory estimate, Section 4.0 makes recommendations about the safety status of the tank and additional sampling needs. The appendices contain supporting data and information. Appendix A contains historical information for 241-T-201 to T-204, including surveillance information, records pertaining to waste transfers and tank operations, and expected tank contents derived from a process knowledge-based computer program. Appendix B summarizes sampling events, sample data obtained before 1989, and the most current sampling results. Appendix C reports the statistical analysis and numerical manipulation of data used in issue resolution. Appendix D contains the evaluation to establish the best-basis for the inventory estimate and the statistical analysis performed for this evaluation. Appendix E is a bibliography that resulted from an in-depth literature search of all known information sources applicable to tanks 241-T-201, -T-202, -T-203, and -T-204. The reports listed in Appendix E are available in the Tank Characterization and Safety Resource Center

  2. Factors Associated with Advanced Inferior Vena Cava Filter Removals: A Single-Center Retrospective Study of 203 Patients Over 7 Years

    International Nuclear Information System (INIS)

    Dowell, Joshua D.; Wagner, Daniel; Elliott, Eric; Yildiz, Vedat O.; Pan, Xueliang

    2016-01-01

    PurposeTo identify factors associated with advanced inferior vena cava filter (IVCF) retrieval to raise awareness on technical considerations, retrieval efficiency, and patient safety.Materials and MethodsA single-center retrospective review was performed of 203 consecutive retrievable IVC filters placed between 2007 and 2014. Attempted retrievals were classified as advanced if the routine “snare and sheath” technique was initially unsuccessful after multiple attempts, or an alternate endovascular maneuver or access site was utilized. Patient and filter characteristics were recorded.Results203 attempted retrievals were reviewed (48.7 % male, 51.2 % female, mean age 52.7 years, mean dwell time 109 days). Advanced retrievals were observed in 20 patients (9.8 %) (15 females, 5 males). Fluoroscopy time (p ≤ 0.01, 34.3 ± 21.1 and 5.3 ± 4.5 min for advanced retrievals and routine retrievals respectively, same below), gender (p = 0.031), and retrieval tilt angle (p ≤ 0.01, 5.7 ± 5.10° vs. 11.9 ± 11.03°) were associated with advanced retrievals. Females were 3.16 times more likely to have an advanced retrieval performed than males with a significantly higher tilt angle in those with advanced retrieval. History of cancer (p = 0.502), dwell time (p = 0.916), retrieval caval diameter (p = 0.053), placement caval diameter (p = 0.365), filter type (p = 0.710), strut perforation (p = 0.506), placement tilt angle (p = 0.311), and age (p = 0.558) were not found significantly associated with advanced retrievals.ConclusionsWomen are at increased risk for advanced filter retrieval secondary to a significant change in filter tilt over time compared to men, independent of filter type or competing demographic or filter risks, likely placing them at increased risk for higher procedural fluoroscopy times

  3. Factors Associated with Advanced Inferior Vena Cava Filter Removals: A Single-Center Retrospective Study of 203 Patients Over 7 Years

    Energy Technology Data Exchange (ETDEWEB)

    Dowell, Joshua D., E-mail: Joshua.Dowell@osumc.edu; Wagner, Daniel, E-mail: Daniel.Wagner@osumc.edu; Elliott, Eric, E-mail: Eric.Elliott@osumc.edu [The Ohio State University Wexner Medical Center, Department of Radiology, Division of Interventional Radiology (United States); Yildiz, Vedat O., E-mail: Vedat.Yildiz@osumc.edu; Pan, Xueliang, E-mail: Jeff.Pan@osumc.edu [The Ohio State University Wexner Medical Center, Center for Biostatistics (United States)

    2016-02-15

    PurposeTo identify factors associated with advanced inferior vena cava filter (IVCF) retrieval to raise awareness on technical considerations, retrieval efficiency, and patient safety.Materials and MethodsA single-center retrospective review was performed of 203 consecutive retrievable IVC filters placed between 2007 and 2014. Attempted retrievals were classified as advanced if the routine “snare and sheath” technique was initially unsuccessful after multiple attempts, or an alternate endovascular maneuver or access site was utilized. Patient and filter characteristics were recorded.Results203 attempted retrievals were reviewed (48.7 % male, 51.2 % female, mean age 52.7 years, mean dwell time 109 days). Advanced retrievals were observed in 20 patients (9.8 %) (15 females, 5 males). Fluoroscopy time (p ≤ 0.01, 34.3 ± 21.1 and 5.3 ± 4.5 min for advanced retrievals and routine retrievals respectively, same below), gender (p = 0.031), and retrieval tilt angle (p ≤ 0.01, 5.7 ± 5.10° vs. 11.9 ± 11.03°) were associated with advanced retrievals. Females were 3.16 times more likely to have an advanced retrieval performed than males with a significantly higher tilt angle in those with advanced retrieval. History of cancer (p = 0.502), dwell time (p = 0.916), retrieval caval diameter (p = 0.053), placement caval diameter (p = 0.365), filter type (p = 0.710), strut perforation (p = 0.506), placement tilt angle (p = 0.311), and age (p = 0.558) were not found significantly associated with advanced retrievals.ConclusionsWomen are at increased risk for advanced filter retrieval secondary to a significant change in filter tilt over time compared to men, independent of filter type or competing demographic or filter risks, likely placing them at increased risk for higher procedural fluoroscopy times.

  4. Structures of Ta22W4O67 and Ta74W6O203. Pt. 1. Refined structural models using synchrotron radiation

    International Nuclear Information System (INIS)

    Schmid, S.

    1995-01-01

    The crystal structures of Ta 22 W 4 O 67 [M r = 5788.19, a = 6.1485 (5), b = 47.6205 (12), c = 3.8559 (3) A, γ = 90.04 (1) , space group = C112/m (non-standard setting), Z = 1, D x = 8.513 g cm -3 , F(000) = 2438] and Ta 74 W 6 O 203 [M r = 17741.06, a = 6.1664 (5), b = 29.2717 (14), c = 3.8731 (2) A, space group = Pbam (no. 55), Z = 0.2, D x = 8.428 g cm -3 , F(000) 1494] were determined using synchrotron radiation at four different wavelengths below the Ta L III edge [λ = 1.2741 (-146 eV), λ = 1.2586 (-26 eV), λ = 1.2571 (-14 eV) and λ = 1.2563 A (-8 eV)]. The collection of data immediately below the Ta L III edge at -8 eV enabled resolution of Ta and W of up to eight electrons, which assisted in the refinement of Ta/W ordering for both structures. Bond valence arguments have been used to locate oxygen vacancies required by the formulae. From the largest data set for Ta 22 W 4 O 67 (λ = 1.2741 A), a final value of 0.0481 for R 1 = Σ parallel F obs (h)vertical stroke - vertical stroke F calc (h) parallel /Σvertical stroke F obs (h)vertical stroke was obtained for 3082 unmerged reflections with I(h) > 3σ[I(h)] and for Ta 74 W 6 O 203 (λ = 1.2563 A) a final value of 0.0571 for R 1 was obtained for 5675 unmerged reflections. The two structures are described from a conventional polyhedral perspective as 13- and 8-times superstructures occurring in the solid solution (1-x)Ta 2 O 5 xWO 3 , 0 ≤ x ≤ 0.267. (orig.)

  5. Depth profile of production yields of {sup nat}Pb(p, xn) {sup 206,205,204,203,202,201}Bi nuclear reactions

    Energy Technology Data Exchange (ETDEWEB)

    Mokhtari Oranj, Leila [Division of Advanced Nuclear Engineering, POSTECH, Pohang 37673 (Korea, Republic of); Jung, Nam-Suk; Kim, Dong-Hyun; Lee, Arim; Bae, Oryun [Pohang Accelerator Laboratory, POSTECH, Pohang 37673 (Korea, Republic of); Lee, Hee-Seock, E-mail: lee@postech.ac.kr [Pohang Accelerator Laboratory, POSTECH, Pohang 37673 (Korea, Republic of)

    2016-11-01

    Experimental and simulation studies on the depth profiles of production yields of {sup nat}Pb(p, xn) {sup 206,205,204,203,202,201}Bi nuclear reactions were carried out. Irradiation experiments were performed at the high-intensity proton linac facility (KOMAC) in Korea. The targets, irradiated by 100-MeV protons, were arranged in a stack consisting of natural Pb, Al, Au foils and Pb plates. The proton beam intensity was determined by activation analysis method using {sup 27}Al(p, 3p1n){sup 24}Na, {sup 197}Au(p, p1n){sup 196}Au, and {sup 197}Au(p, p3n){sup 194}Au monitor reactions and also by Gafchromic film dosimetry method. The yields of produced radio-nuclei in the {sup nat}Pb activation foils and monitor foils were measured by HPGe spectroscopy system. Monte Carlo simulations were performed by FLUKA, PHITS/DCHAIN-SP, and MCNPX/FISPACT codes and the calculated data were compared with the experimental results. A satisfactory agreement was observed between the present experimental data and the simulations.

  6. Depth profiles of production yields of natPb(p, xn206,205,204,203,202 Bi reactions using 100-MeV proton beam

    Directory of Open Access Journals (Sweden)

    Oranj Leila Mokhtari

    2017-01-01

    Full Text Available In this study, results of the experimental study on the depth profiles of production yields of 206,205,204,203,202Bi radio-nuclei in the natural Pb target irradiated by a 100-MeV proton beam are presented. Irradiation was performed at proton linac facility (KOMAC in Korea. The target, irradiated by 100-MeV protons, was arranged in a stack consisting of natural Pb, Al, Au foils and Pb plates. The proton beam intensity was determined by activation analysis method using 27Al(p, 3p1n24Na, 197Au(p, p1n196Au, and 197Au(p, p3n194Au monitor reactions and also using dosimetry method by a Gafchromic film. The production yields of produced Bi radio-nuclei in the natural Pb foils and monitor reactions were measured by gamma-ray spectroscopy. Monte Carlo simulations were performed by FLUKA, PHITS, and MCNPX codes and compared with the measurements in order to verify validity of physical models and nuclear data libraries in the Monte Carlo codes. A fairly good agreement was observed between the present experimental data and the simulations by FLUKA, PHITS, and MCNPX. However, physical models and the nuclear data relevant to the end of range of protons in the codes need to be improved.

  7. Specific ability of sulfur-ligands on removal of 203Hg-labeled organomercury from hemoglobin in comparison with nitrogen-ligands

    International Nuclear Information System (INIS)

    Hojo, Yasuji; Sugiura, Yukio; Tanaka, Hisashi

    1975-01-01

    Removal of 203 Hg-labeled organomercurials, bound to sulfhydryl groups of hemoglobin, by various chelating agents was investigated by the use of equilibrium dialysis. Organomercurials employed were chlormerodrin, methylmercury, ethylmercury and phenylmercury compounds. Higher and more specific effects of the sulfur-ligands, such as penicillamine and glutathione, on removal of organomercurial were found as compared with those of the nitrogen-ligands such as EDTA, glycine and polymethylenediamines. Linear correlation was observed between the degree of organomercury elimination from hemoglobin and the stability constant (log K 1 ) of 1:1 organomercury complex in both the sulfur- and nitrogen-ligand systems and at the same value of log K 1 , the elimination-effect of sulfur-ligands was extremely greater than that of the nitrogen-ligands. The relationship between the average percentage of removal and the Taft's polar substituent constant of organic moiety of the metal was also linear among the organomercury compounds other than chlormerodrin. The average removal percentage by sulfur-ligands increased in the order, ethylmercury>methylmercury>phenylmercury, while that of the nitrogen-ligands was not different among the organomercurials investigated. In addition, direct ligand-exchange reaction between hemoglobin-SH and the ligand coordinating-atom (S or N) against organomercurials rather than Ssub(N2) reaction via the ternary complex, hemoglobin-S-RHg-ligand, is postulated. (auth.)

  8. Flexible synthetic routes to poison-frog alkaloids of the 5,8-disubstituted indolizidine-class I: synthesis of common lactam chiral building blocks and application to the synthesis of (--203A, (--205A, and (--219F

    Directory of Open Access Journals (Sweden)

    Spande Thomas F

    2007-09-01

    Full Text Available Abstract Background The 5,8-disubstituted indolizidines are the largest class of poison-frog alkaloids found in anuran skin, and are of considerable interest because of their inhibitory effects on the neuronal nicotinic acetylcholine receptors. Many synthetic strategies for the construction of this nucleus have been reported: however, a flexible route has not been reported to date. Results Synthesis of lactam chiral building blocks for the flexible synthesis of the title alkaloids has been achieved using a Michael-type conjugate addition reaction to a chiral cyclic enamine ester as the key step in constructing the trisubstituted piperidine ring system. To demonstrate the usefulness of these chiral building blocks, syntheses of (--203A, (--205A from 1, and (--219F from 2 have been achieved. Conclusion The total synthesis of (--203A, (--205A, and (--219F was achieved, and the absolute stereochemistry of natural 203A was determined to be 5S, 8R, 9S. In addition, the relative stereochemistry of natural 219F was determined.

  9. 211 At-labeled agents for alpha-immunotherapy: On the in vivo stability of astatine-agent bonds

    OpenAIRE

    Ayed , Tahra ,; Pilmé , Julien; Tézé , David; Bassal , Fadel ,; Barbet , Jacques ,; Chérel , Michel; Champion , Julie ,; Maurice , Rémi; Montavon , Gilles ,; Galland , Nicolas ,

    2016-01-01

    International audience; The application of 211 At to targeted cancer therapy is currently hindered by the rapid deastatination that occurs in vivo. As the deastatination mechanism is unknown, we tackled this issue from the viewpoint of the intrinsic properties of At-involving chemical bonds. An apparent correlation has been evidenced between in vivo stability of 211 At-labeled compounds and the AtÀR (R ¼ C, B) bond enthalpies obtained from relativistic quantum mechanical calculations. Further...

  10. Preparation and biological evaluation of an astatine-211 labeled biotin conjugate: Biotinyl-3-[211At]astatoanilide

    International Nuclear Information System (INIS)

    Foulon, Catherine F.; Schoultz, Bent W.; Zalutsky, Michael R.

    1997-01-01

    Biotinyl-3-[ 211 At]astatoanilide ([ 211 At]AtBA) was prepared in more than 80% yield by destannylation. In vitro, [ 211 At]AtBA exhibited a high affinity for streptavidin, and was stable after incubation in human serum, cerebrospinal fluid and distilled water, whereas it was rapidly degraded in mouse serum. HPLC analysis showed that the main degradation pathway in mouse serum was the cleavage of [ 211 At]astatoaniline. In mice, [ 211 At]AtBA and its 125 I-labeled analogue cleared rapidly from most tissues; however, there was some evidence for dehalogenation of both tracers

  11. Evaluation of Novel Wet Chemistry Separation and Purification Methods to Facilitate Automation of Astatine-­211 Isolation

    Energy Technology Data Exchange (ETDEWEB)

    Wilbur, Daniel Scott [Univ. of Washington, Seattle, WA (United States)

    2016-07-19

    This research is a collaborative effort between the research groups of the PIs, Dr. D. Scott Wilbur in the Department of Radiation Oncology at the University of Washington (UW) and Matthew O’Hara at the Pacific Northwest National Laboratory (PNNL). In this report only those studies conducted at UW and the budget information from UW will be reported. A separate progress and financial report will be provided by PNNL. This final report outlines the experiments (Tasks) conducted and results obtained at UW from July 1, 2013 thru June 30, 2016 (2-­year project with 1 year no-­cost extension). The report divides the information on the experiments and results obtained into the 5 specific objectives of the research efforts and the Tasks within those objectives. This format is used so that it is easy to see what has been accomplished in each area. A brief summary of the major findings from the studies is provided below. Summary of Major Findings from Research/Training Activities at UW: Anion and cation exchange columns did not provide adequate 211At capture and/or extraction results under conditions studied to warrant further evaluation; PEG-­Merrifield resins containing mPEG350, mPEG750, mPEG2000 and mPEG5000 were synthesized and evaluated; All of the mPEG resins with different sized mPEG moieties conjugated gave similar 211At capture (>95%) from 8M HCl solutions and release with conc. NH4OH (~50-­80%), but very low quantities were released when NaOH was used as an eluent; Capture and release of 211At when loading [211At]astatate appeared to be similar to that of [211At]astatide on PEG columns, but further studies need to be conducted to confirm that; Capture of 211At on PEG columns was lower (e.g. 80-­90%) from solutions of 8M HNO3, but higher capture rates (e.g. 99%) can be obtained when 10M HNO3 is mixed with an equal quantity of 8M HCl; Addition of reductants to the 211At solutions did not appear to change the percent capture, but may have an effect on the % extracted; There was some indication that the PEG-­Merrifield resins could be saturated (perhaps with Bi) resulting in lower capture percentages, but more studies need to be done to confirm that; A target dissolution chamber, designed and built at PNNL, works well with syringe pumps so it can be used in an automated system; Preliminary semi-­automated 211At isolation studies have been conducted with full-scale target dissolution and 211At isolation using a PEG column on the Hamilton automated system gave low overall recoveries, but HNO3 was used (rather than HCl) for loading the 211At and flow rates were not optimized; Results obtained using PEG columns are high enough to warrant further development on a fully automated system; Results obtained also indicate that additional studies are warranted to evaluate other types of columns for 211At separation from bismuth, which allow use of HNO3/HCl mixtures for loading and NaOH for eluting 211At. Such a column could greatly simplify the overall isolation process and make it easier to automate.

  12. The structural basis of the dominant negative phenotype of the Gαi1β1γ2 G203A/A326S heterotrimer

    Science.gov (United States)

    Liu, Ping; Jia, Ming-zhu; Zhou, X Edward; De Waal, Parker W; Dickson, Bradley M; Liu, Bo; Hou, Li; Yin, Yan-ting; Kang, Yan-yong; Shi, Yi; Melcher, Karsten; Xu, H Eric; Jiang, Yi

    2016-01-01

    Aim: Dominant negative mutant G proteins have provided critical insight into the mechanisms of G protein-coupled receptor (GPCR) signaling, but the mechanisms underlying the dominant negative characteristics are not completely understood. The aim of this study was to determine the structure of the dominant negative Gαi1β1γ2 G203A/A326S complex (Gi-DN) and to reveal the structural basis of the mutation-induced phenotype of Gαi1β1γ2. Methods: The three subunits of the Gi-DN complex were co-expressed with a baculovirus expression system. The Gi-DN heterotrimer was purified, and the structure of its complex with GDP was determined through X-ray crystallography. Results: The Gi-DN heterotrimer structure revealed a dual mechanism underlying the dominant negative characteristics. The mutations weakened the hydrogen bonding network between GDP/GTP and the binding pocket residues, and increased the interactions in the Gα-Gβγ interface. Concomitantly, the Gi-DN heterotrimer adopted a conformation, in which the C-terminus of Gαi and the N-termini of both the Gβ and Gγ subunits were more similar to the GPCR-bound state compared with the wild type complex. From these structural observations, two additional mutations (T48F and D272F) were designed that completely abolish the GDP binding of the Gi-DN heterotrimer. Conclusion: Overall, the results suggest that the mutations impede guanine nucleotide binding and Gα-Gβγ protein dissociation and favor the formation of the G protein/GPCR complex, thus blocking signal propagation. In addition, the structure provides a rationale for the design of other mutations that cause dominant negative effects in the G protein, as exemplified by the T48F and D272F mutations. PMID:27498775

  13. Estrategias de enseñanza para el curso EIF-203 Estructuras discretas para informática a través del uso de las redes sociales Facebook y Twitter

    Directory of Open Access Journals (Sweden)

    Enrique Vílchez Quesada

    2014-05-01

    Full Text Available Recibido 29 de octubre de 2013 • Corregido 2 de marzo de 2014 • Aceptado 2 de abril de 2014. Este artículo corresponde a un trabajo científico derivado del proyecto de investigación en docencia adscrito a la Escuela de Informática de la Universidad Nacional de Costa Rica (UNA, titulado: Facebook como herramienta de enseñanza y aprendizaje para el curso EIF-203 Estructuras discretas para informática a través del uso de cuadernos interactivos. El objetivo general del proyecto consistió en analizar técnica y pedagógicamente la red social Facebook como un entorno de enseñanza y aprendizaje. En este documento se presenta una serie de estrategias de enseñanza creadas para complementar la docencia en el contexto del curso EIF-203, utilizando como plataforma educativa las redes sociales Facebook y Twitter. Las estrategias compartidas constituyen una intensa búsqueda de nuevas metodologías para mejorar los procesos de enseñanza e, idealmente, los procesos de aprendizaje en la materia EIF-203, integrada dentro del plan de estudios de la carrera Ingeniería en Sistemas de Información de la UNA. Estas fueron implementadas en un grupo de 31 estudiantes de la Sede Interuniversitaria de Alajuela, durante el I semestre 2013. Lo anterior permitió evaluar las estrategias de enseñanza desarrolladas utilizando una metodología de carácter cuantitativo. El instrumento empleado (un cuestionario se validó mediante una prueba de fiabilidad “Alfa de Cronbach” recurriendo a una muestra de 65 participantes. Los resultados obtenidos forman parte del análisis expuesto en el presente trabajo.

  14. Search for squarks and gluinos using final states with jets and missing transverse momentum with the ATLAS experiment in 20.3 fb-1 of √s=8 TeV proton-proton collisions

    CERN Document Server

    Consorti, V; The ATLAS collaboration

    2013-01-01

    A search for squarks and gluinos in final states containing high-pT jets, missing transverse momentum and no electrons or muons is presented. The data were recorded in 2012 by the ATLAS experiment in √s=8 TeV proton-proton collisions at the Large Hadron Collider, with a total integrated luminosity of 20.3 fb−1. No significant excess above the Standard Model expectation is observed. Results are interpreted by setting limits in several simplified and complex SUSY scenarios.

  15. Study and construction of a {beta}-spectrometer of uniform axial magnetic field fitted with a {beta}-{gamma} coincidence selector. Study of the {beta} spectra of {sup 32}P, {sup 203}Hg, {sup 198}Au. Measurement of the conversion coefficients of {sup 203}Ti and of {sup 198}Hg; Etude et realisation d'un spectrometre-{beta} a champ magnetique axial uniforme, muni d'un selecteur de coincidence {beta}-{gamma}. Etude des spectres {beta} du {sup 32}p, {sup 203}Hg, {sup 198}Au. Mesure des coefficients de conversion du {sup 203}Ti et du {sup 198}Hg

    Energy Technology Data Exchange (ETDEWEB)

    Parsignault, D [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1964-06-01

    In the first part is given the principle of the beta spectrometer with uniform axial field using systematically the idea of caustics. The apparatus is described and its properties compared to those deduced from trajectory calculations. The {beta}-ray and {gamma}-ray detectors and the device for selecting coincidences with a 2 {tau} resolution of 5 nanoseconds are also presented. In the second part, the spectrometer is used for studying reference elements and the most accurate results are confirmed. The {beta} spectrum of {sup 60}Co has a statistical form with an accuracy of 1 per cent; the maximum energy E{sub 0} is 316.5 {+-} 1.5 keV. That of the 7/2 + {yields} 11/2 transition for {sup 137}Cs has a unique form, once forbidden. E{sub 0}= 522 {+-} 3 keV. Conversion coefficients {alpha}{sub k} = 96 {+-} 1 X 10{sup -3} {alpha}L + M + N = 20.9 {+-} 0.5 X 10{sup -3}. The two {beta} spectra of {sup 59}Fe, separated by coincidence with the gamma, have the statistical form E{sub 0} = 462 {+-} 2 keV (55.1 + 0,3 per cent) and E{sub 1} = 275 {+-} 4 keV (44.9 {+-} 0.3 per cent). It is then verified whether the l selection rule is apparent in the shape of the phosphorus 32 beta spectrum. It is found in fact that it is not of statistical shape and its shape coefficient is determined. For a theoretical interpretation it is necessary to have better approximations than those generally used and this interpretation will not be unique. This work has also made it possible to show that the source contains a small proportion of {sup 33}P. The study of the {sup 203}Hg {beta} spectrum followed by the 279 keV gamma spectrum is designed to determine the conversion coefficients. The interior spectrum of gold 198 is not of statistical shape either. The form coefficient is determined together with the conversion coefficients which are in slight disagreement with those calculated by Rose or Sliv. An interpretation of the spectrum is put forward which proposes an imperfect compensation for the

  16. Study and construction of a {beta}-spectrometer of uniform axial magnetic field fitted with a {beta}-{gamma} coincidence selector. Study of the {beta} spectra of {sup 32}P, {sup 203}Hg, {sup 198}Au. Measurement of the conversion coefficients of {sup 203}Ti and of {sup 198}Hg; Etude et realisation d'un spectrometre-{beta} a champ magnetique axial uniforme, muni d'un selecteur de coincidence {beta}-{gamma}. Etude des spectres {beta} du {sup 32}p, {sup 203}Hg, {sup 198}Au. Mesure des coefficients de conversion du {sup 203}Ti et du {sup 198}Hg

    Energy Technology Data Exchange (ETDEWEB)

    Parsignault, D. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1964-06-01

    In the first part is given the principle of the beta spectrometer with uniform axial field using systematically the idea of caustics. The apparatus is described and its properties compared to those deduced from trajectory calculations. The {beta}-ray and {gamma}-ray detectors and the device for selecting coincidences with a 2 {tau} resolution of 5 nanoseconds are also presented. In the second part, the spectrometer is used for studying reference elements and the most accurate results are confirmed. The {beta} spectrum of {sup 60}Co has a statistical form with an accuracy of 1 per cent; the maximum energy E{sub 0} is 316.5 {+-} 1.5 keV. That of the 7/2 + {yields} 11/2 transition for {sup 137}Cs has a unique form, once forbidden. E{sub 0}= 522 {+-} 3 keV. Conversion coefficients {alpha}{sub k} = 96 {+-} 1 X 10{sup -3} {alpha}L + M + N = 20.9 {+-} 0.5 X 10{sup -3}. The two {beta} spectra of {sup 59}Fe, separated by coincidence with the gamma, have the statistical form E{sub 0} = 462 {+-} 2 keV (55.1 + 0,3 per cent) and E{sub 1} = 275 {+-} 4 keV (44.9 {+-} 0.3 per cent). It is then verified whether the l selection rule is apparent in the shape of the phosphorus 32 beta spectrum. It is found in fact that it is not of statistical shape and its shape coefficient is determined. For a theoretical interpretation it is necessary to have better approximations than those generally used and this interpretation will not be unique. This work has also made it possible to show that the source contains a small proportion of {sup 33}P. The study of the {sup 203}Hg {beta} spectrum followed by the 279 keV gamma spectrum is designed to determine the conversion coefficients. The interior spectrum of gold 198 is not of statistical shape either. The form coefficient is determined together with the conversion coefficients which are in slight disagreement with those calculated by Rose or Sliv. An interpretation of the spectrum is put forward which proposes an imperfect compensation for the

  17. Millimeter- and submillimeter-wave surveys of Orion A emission lines in the ranges 200.7-202.3, 203.7-205.3, and 330-360 GHz

    International Nuclear Information System (INIS)

    Jewell, P.R.; Hollis, J.M.; Lovas, F.J.; Snyder, L.E.

    1989-01-01

    A continuous spectral line survey of the Orion A position from 330.5 to 360.1 GHz was carried out. This survey covers nearly the entire 870 micron atmospheric window accessible from ground-based observations. Approximately 160 distinct spectral features composed of about 180 lines were detected, 29 of which could not be readily identified. In addition, Orion A from 200.7 to 202.3 GHz and from 203.7 to 205.3 GHz and 42 distinct new spectral lines were detected, including four that are unidentified at present. These data sets are the first thorough survey results in these spectral regions. The new interstellar lines in the survey bands are tabulated and displayed graphically. Moreover, the data are being made available to the Astronomical Data Center at the Goddard Space Flight Center for distribution by request to the astronomical community. 14 refs

  18. A comparative study of shadow shield whole body monitor incorporated with 203 mm dia. x 102 mm thick and 102 mm dia. x 76 mm thick NaI(TI) detectors

    International Nuclear Information System (INIS)

    Sankhla, Rajesh; Singh, I.S.; Rao, D.D.

    2016-01-01

    The whole body counting using Shadow Shield Whole Body Monitor (SSWBM) proved to be a popular method for assessment of internal contamination due to high energy gamma (E>200 keV) emitting radio nuclides that got inadvertently incorporated in the occupational workers. Currently ∼ 5 SSWBMs are operational at various DAE nuclear facilities throughout the country. The shielding of SSWBMs are said to be designed for 102 mm x 76 mm NaI(Tl) detector and over a period of time, the same concept is being followed. At present, the number of subjects monitored per annum has increased significantly compared to earlier years due to the increase in nuclear facilities at different sites and also increase in number of contract personnel. Aim of this study is to develop/upgrade the existing SSWBMs to increase their capabilities in terms of throughput without compromising on sensitivity. This work includes response studies of individual detectors of sizes 102 mm x 76 mm and 203 mm x 102 mm housed in SSWBM in terms of background, efficiency and Minimum Detection Activity (MDA) for different gamma emitting radio nuclides using Bhabha Atomic Research Centre reference Bottle Mannequin ABsorption (BOMAB) phantom

  19. Search for Vector-like Quarks using trilepton and same-sign dilepton events in 20.3 fb$^{-1}$ of proton-proton collisions at $\\sqrt{s}$ = 8 TeV with the ATLAS detector

    CERN Document Server

    O'Grady, Fionnbarr

    A search is presented for vector-like quarks using 20.3 ​fb$^{-1}$​ of proton-proton collisions at a center-of-mass energy of ​$\\sqrt{s}$​ = 8 TeV collected with the ATLAS detector​ ​in 2012 at the CERN Large Hadron Collider. Vector-like quarks are predicted to​ ​exist in many theories beyond the Standard Model of particle physics that attempt​ ​to resolve the hierarchy problem. Events are selected containing jets including at​ ​​least one b-jet, sizable missing transverse momentum, large scalar sum of the jet​ ​and lepton transverse momenta, and either three leptons or two leptons with the​ ​same electric charge. Standard Model processes rarely produce this ​final state and​ ​production of vector-like quarks would lead to an enhanced rate of such events. The​ ​data are interpreted in the context of a variety of models including pair production​ ​of vector-like quarks T and B, which have the same electric charge as the corresponding Standard Model quarks and can...

  20. Successive composition of two laser channels upon excitation of He-Ar-Xe (2.03 μm) and Ar-Xe (1.73 μm) mixtures by uranium fission fragments

    International Nuclear Information System (INIS)

    Pikulev, A A; Tsvetkov, V M; Sosnin, P V; Sinyanskii, A A

    2009-01-01

    The operation efficiency of the scheme with successive composition of two laser channels upon excitation of the active medium by uranium-235 fission fragments is studied experimentally and numerically. For the He:Ar:Xe = 380:380:1 mixture (at a pressure of 1 atm and the lasing wavelength λ = 2.03 μm) the maximum lasing power of a double channel (1 kW) is almost twice that of a single channel (540 W). Calculations show that in the case of ideal composition (without losses on mirrors) the lasing power of the double channel can be increased to 1.2 kW. For the Ar:Xe = 380:1 mixture (the pressure is 0.5 atm, λ = 1.73 μm) the maximum lasing power of the double channel (620 W) is slightly above that of the single channel (520 W), which is caused by the losses on aluminum mirrors employed for channel doubling and by a negative effect of optical inhomogeneities. In the case of ideal composition, the lasing power can be increased to 830 W. (lasers)

  1. Mead acid (20:3n-9) and n-3 polyunsaturated fatty acids are not associated with risk of posterior longitudinal ligament ossification: results of a case-control study.

    Science.gov (United States)

    Hamazaki, Kei; Kawaguchi, Yoshiharu; Nakano, Masato; Yasuda, Taketoshi; Seki, Shoji; Hori, Takeshi; Hamazaki, Tomohito; Kimura, Tomoatsu

    2015-05-01

    Ossification of the posterior longitudinal ligament (OPLL) involves the replacement of ligamentous tissue with ectopic bone. Although genetics and heritability appear to be involved in the development of OPLL, its pathogenesis remains to be elucidated. Given previous findings that 5,8,11-eicosatrienoic acid [20:3n-9, Mead acid (MA)] has depressive effects on osteoblastic activity and anti-angiogenic effects, and that n-3 polyunsaturated fatty acids (PUFAs) have a preventive effect on heterotopic ossification, we hypothesized that both fatty acids would be involved in OPLL development. To examine the biological significance of these and other fatty acids in OPLL, we conducted this case-control study involving 106 patients with cervical OPLL and 109 age matched controls. Fatty acid composition was determined from plasma samples by gas chromatography. Associations between fatty acid levels and incident OPLL were evaluated by logistic regression. Contrary to our expectations, we found no significant differences between patients and controls in the levels of MA or n-3 PUFAs (e.g., eicosapentaenoic acid and docosahexaenoic acid). Logistic regression analysis did not reveal any associations with OPLL risk for MA or n-3 PUFAs. In conclusion, no potential role was found for MA or n-3 PUFAs in ectopic bone formation in the spinal canal. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Inactivation of human osteosarcoma cells in vitro by 211At-TP-3 monoclonal antibody: Comparison with astatine-211 and external-beam X rays

    International Nuclear Information System (INIS)

    Larsen, R.H.; Bruland, O.S.; Hoff, P.; Alstad, J.; Lindmo, T.; Rofstad, E.K.

    1994-01-01

    The potential usefulness of α-particle radioimmunotherapy in the treatment of osteosarcoma was studied in vitro by using the monoclonal antibody TP-3 and cells of three human osteosarcoma cell lines (OHS, SAOS and KPDX) differing in antigen expression. Cell survival curves were established after treatment with (a) 211 At-TP-3 of different specific activities, (b) 211 At-labeled bovine serum albumin (BSA), (c) free 211 At and (d) external-beam X rays. The three osteosarcoma cell lines showed similar survival curves, whether treated with external-beam X rays, 211 At-BSA or free 211 At. The D o 's were lower for free 211 At than for 211 At-BSA. The survival curves for 211 At-TP-3 treatment, on the other hand, differed significantly among the cell lines, suggesting that sensitivity to 211 At-TP-3 treatment was governed by cellular properties other than sensitivity to external-beam X rays. The cellular property most important for sensitivity to 211 At-TP-3 treatment was the antigen expression. Cell inactivation after 211 At-TP-3 treatment increased substantially with increasing specific activity of the 211 At-TP-3. At high specific activities, the cytotoxic effect of 211 At-TP-3 was significantly higher than that of 211 At-BSA. In conclusion, 211 At-TP-3 has the potential to give clinically favorable therapeutic ratios in the treatment of osteosarcoma. 39 refs., 5 figs., 2 tabs

  3. 24 CFR 904.203 - Planning.

    Science.gov (United States)

    2010-04-01

    ... be recognized that most of the objectives stated require specialized instructional skill and content...-administered or contracted, the LHA shall consult with HUD for advice and information on programs, qualified... contracted to an outside group, proposals shall be secured either by public advertising or by sending...

  4. 14 CFR 120.203 - General.

    Science.gov (United States)

    2010-01-01

    ... applicable to employers by this subpart. (c) Employer responsibility. As an employer, you are responsible for... employees who perform safety-sensitive functions in aviation. (b) Alcohol testing procedures. Each employer...

  5. Auto Body Repair 103, 203, 303.

    Science.gov (United States)

    Manitoba Dept. of Education, Winnipeg.

    As part of the high school vocational industrial program in Manitoba, this course has been designed to provide students with the foundation for a career in auto-body repair. The program introduces and provides opportunities for student skill development in the theories and techniques of tool and equipment operation, metallurgy and welding, damage…

  6. 5 CFR 179.203 - Definitions.

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    2010-01-01

    ... Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS CLAIMS COLLECTION STANDARDS... Office of Personnel Management. Paying agency means the agency of the Federal Government which employs... the creditor agency and the paying agency. Payroll office means the payroll office in the paying...

  7. 24 CFR 3282.203 - DAPIA services.

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    2010-04-01

    ... and methods of installing required safety equipment, (6) Wiring diagrams, including circuit allocation..., the type of wiring used, and wiring methods, (7) Details showing the design of air supply and return.../or energy requirements, (10) Detailed manufacturer installation instructions including specifications...

  8. 24 CFR 203.205 - Plan coverage.

    Science.gov (United States)

    2010-04-01

    ... damaged during installation or improperly installed by the builder. The plan must also cover structural... warranty against defects in the wiring, piping and ductwork in the electrical, plumbing, heating, cooling...

  9. 32 CFR 203.3 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... for making environmental decisions for their component or the director of the Defense Agencies. DoD... the identified decision authority designated for the installation. A completed application will... legally authorized to obligate the organization executing a TAPP purchase order to the terms and...

  10. 28 CFR 20.3 - Definitions.

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    2010-07-01

    ... have elected not to refer a matter to a prosecutor or that a prosecutor has elected not to commence... FIRS Privacy Act System Notice periodically published in the Federal Register for further details. (m.... See the NCIC Privacy Act System Notice periodically published in the Federal Register for further...

  11. PODAAC-SMP20-3SMCS

    Data.gov (United States)

    National Aeronautics and Space Administration — The version 2.0 SMAP-SSS level 3, monthly gridded product is based on the first release of the validated standard mapped sea surface salinity (SSS) data from the...

  12. PODAAC-SMP20-3SPCS

    Data.gov (United States)

    National Aeronautics and Space Administration — The version 2.0 SMAP-SSS level 3, 8-Day running mean gridded product is based on the first release of the validated standard mapped sea surface salinity (SSS) data...

  13. 40 CFR 89.203 - General provisions.

    Science.gov (United States)

    2010-07-01

    ... nonconformity determined by a Selective Enforcement Audit (SEA) or by recall (in-use) testing. However, in the... nonconformity determined by a Selective Enforcement Audit (SEA) or by recall (in-use) testing. However, in the... not be used to remedy nonconformity determined by a Selective Enforcement Audit (SEA) or by recall (in...

  14. Publications | Page 203 | IDRC - International Development ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    IDRC works with developing-country researchers and institutions to build local capacity through funding, knowledge sharing, and training. ... Policy-oriented research reflects not only a government''s or intergovernmental organization''s recognition of issues, and potential ways to address them, but also determines flows of ...

  15. 24 CFR 92.203 - Income determinations.

    Science.gov (United States)

    2010-04-01

    ... accordance with § 92.252(h): (i) Examine the source documents evidencing annual income (e.g., wage statement... annual income; or alternatively, the statement must indicate the current dollar limit for very low- or low-income families for the family size of the tenant and state that the tenant's annual income does...

  16. 5 CFR 351.203 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... for a position covered by a wage-board or similar wage-determining procedure, such as provided in the definition of representative rate for Federal Wage System positions in 5 CFR 532.401 of this chapter; (3) For... low priority program or to a vacant position. [51 FR 319, Jan. 3, 1986, as amended at 58 FR 65533, Dec...

  17. 48 CFR 203.903 - Policy.

    Science.gov (United States)

    2010-10-01

    ... DEFENSE GENERAL IMPROPER BUSINESS PRACTICES AND PERSONAL CONFLICTS OF INTEREST Whistleblower Protections... that the employee reasonably believes is evidence of gross mismanagement of a DoD contract, a gross waste of DoD funds, a substantial and specific danger to public health or safety, or a violation of law...

  18. 15 CFR 20.3 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... means any action using age or an age-related term. (e) Age-related term means a word or words which..., entitlement, loan, cooperative agreement, contract (other than a procurement contract or a contract of...

  19. Publications | Page 203 | IDRC - International Development ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Impact of migrant workers on the tuberculosis transmission : general models and ... to the CCAA program, some 89% of these organizations are based in Africa, ... research into health hazards on smallholder dairy farms in a poor urban area in ...

  20. 47 CFR 90.203 - Certification required.

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    2010-10-01

    ... certificated for use under this part. (1) Effective October 16, 2002, except in the 1427-1432 MHz band, an equipment approval may no longer be obtained for in-hospital medical telemetry equipment operating under the provisions of this part. The requirements for obtaining an approval for medical telemetry equipment after...

  1. 7 CFR 765.203 - Protective advances.

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    2010-01-01

    ... following actions will be charged to the borrower's account: (a) Maintain abandoned security property; (b) Preserve inadequately maintained security; (c) Pay real estate taxes and assessments; (d) Pay property... ground rents; (h) Pay expenses for emergency measures to protect the Agency's collateral; and (i) Protect...

  2. 48 CFR 17.203 - Solicitations.

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    ... higher than the base price only if the acceptance does not prejudice any other offeror; and (2) Limit... normally should allow option quantities to be offered without limitation as to price, and there shall be no limitation as to price if the option quantity is to be considered in the evaluation for award (see 17.206...

  3. 33 CFR 160.203 - Exemptions.

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    ... than 500 gross tons need not submit the International Safety Management (ISM) Code Notice (Entry (7) to... submission requirements. (f) U.S. vessels need not submit the International Ship and Port Facility Code (ISPS...

  4. 10 CFR 600.203 - Applicability.

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    ... Act); (iii) Foster Care and Adoption Assistance (Title IV-E of the Act); (iv) Aid to the Aged, Blind...)(B). (4) Entitlement grants under the following programs of The National School Lunch Act: (i) School... The Child Nutrition Act of 1966: (i) Special Milk (section 3 of the Act), and (ii) School Breakfast...

  5. 40 CFR 170.203 - Exceptions.

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    ... programs sponsored by governmental entities. (b) On livestock or other animals, or in or about animal... plants, including, but not limited to, structural pest control, control of vegetation along rights-of-way...

  6. 33 CFR 203.32 - Policy.

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    ... by the responsible tribal, State, or local official. However, legal responsibility always remains... responsibility. Corps technical advice and assistance, as well as assistance with flood fight operations, can be... the non-Federal interest's responsibility to pay for or perform maintenance if a Stafford Act...

  7. 40 CFR 211.203 - Definitions.

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    2010-07-01

    ... Band Attenuation. The amount of sound reduction determined according to the measurement procedure of... the ear canal, that is sold wholly or in part on the basis of its ability to reduce the level of sound... giving noise reduction effectiveness information appropriate to the product. (p) Manufacturer. As stated...

  8. 48 CFR 203.171-3 - Policy.

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    ... ethics counselor regarding the applicability of post-employment restrictions to activities that the... receiving compensation from the contractor, the post-employment ethics opinion described in paragraph (a) of... procurement, rescission of a contract, or initiation of suspension or debarment proceedings. [74 FR 2409, Jan...

  9. 32 CFR 203.10 - Eligible activities.

    Science.gov (United States)

    2010-07-01

    ... documents. The installation restoration program documents each stage of investigation and decision-making...) MISCELLANEOUS TECHNICAL ASSISTANCE FOR PUBLIC PARTICIPATION (TAPP) IN DEFENSE ENVIRONMENTAL RESTORATION... public's ability to provide advice to decision-makers by improving the public's understanding of overall...

  10. 5 CFR 724.203 - Training obligations.

    Science.gov (United States)

    2010-01-01

    ...) Each agency must develop a written plan to train all of its employees (including supervisors and... materials and method of its training plan. Each agency training plan shall describe: (1) The instructional materials and method of the training, (2) The training schedule, and (3) The means of documenting completion...

  11. 24 CFR 203.200 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES SINGLE... or exterior surfaces if only one or two surfaces are damaged. It does not cover personal property items, not a part of the structure, which are damaged by the defect or as a result of the defect. It...

  12. 32 CFR 203.5 - TAPP process.

    Science.gov (United States)

    2010-07-01

    ... project request, the rationale for that decision will be provided to the RAB/TRC in writing. (d) Purchase...'s environmental restoration or BRAC accounts, considering a number of factors related to the restoration program at the installation and its impact upon the community. These factors include, but are not...

  13. 7 CFR 1416.203 - Eligible livestock.

    Science.gov (United States)

    2010-01-01

    ... hurricane during the disaster period; (iii) Been maintained for commercial use as part of a farming... other than commercial use as part of a farming operation, including but not limited to wild free roaming..., crawfish, equine, sheep goats, swine, poultry or deer; (ii) Died in an eligible county as a direct result...

  14. 47 CFR 13.203 - Examination elements.

    Science.gov (United States)

    2010-10-01

    ... electronic fundamentals and techniques required to adjust, repair, and maintain radio transmitters and... routing and accounting, etc. (5) Element 7: GMDSS radio operating practices. Questions concerning GMDSS...

  15. 33 CFR 203.83 - Additional requirements.

    Science.gov (United States)

    2010-07-01

    ... Public Law 84-99 assistance, but will not be “at no cost to the United States.” Use of another Federal... as protection against erosion. This restriction on use of these authorities does not preclude furnishing flood fight assistance during an emergency. (b) Areas of minor damage, flood control works...

  16. 49 CFR 232.203 - Training requirements.

    Science.gov (United States)

    2010-10-01

    ... satisfaction of the employee's supervisor or designated instructor; (6) An employee hired or working prior to... designation program for its employees that perform brake system inspections, tests, or maintenance. For... related to the performance of the tasks identified. (3) Require all employees to successfully complete a...

  17. 49 CFR 180.203 - Definitions.

    Science.gov (United States)

    2010-10-01

    ... volume, due to application of pressure, that is lost when pressure is released (elastic expansion = total... this section, the word “cylinder” includes UN pressure receptacles. In addition to the definitions..., is not corrosive to the materials of construction of a cylinder (including valve, pressure relief...

  18. 33 CFR 203.62 - Drought assistance.

    Science.gov (United States)

    2010-07-01

    ... of the project sponsor(s). (d) Definitions applicable to this section—(1) Construction. This term.... (2) The financing of the cost of construction of a well by the Corps under this authority should be secured by the project applicant. (3) The project applicant will provide the necessary assurances of local...

  19. 49 CFR 228.203 - Program components.

    Science.gov (United States)

    2010-10-01

    ... daylight savings time; (9) For train employees, require completion of a full record at the end of a duty... restricted to the employee or train crew or signal gang whose time is being recorded, with the following... computer terminals is delayed and the employee has exceeded his or her maximum allowed time on duty. (2) No...

  20. 47 CFR 20.3 - Definitions.

    Science.gov (United States)

    2010-10-01

    ... requires the use of special location-determining hardware and/or software in a portable or mobile phone...-capable handsets. Portable or mobile phones that contain special location-determining hardware and/or... hardware and/or software in the caller's portable or mobile phone. Private Mobile Radio Service. A mobile...

  1. 24 CFR 203.369 - Deficiency judgments.

    Science.gov (United States)

    2010-04-01

    ... commitments issued on or after March 28, 1988, or pursuant to direct endorsement processing where the credit... insured pursuant to firm commitments issued before March 28, 1988, or pursuant to direct endorsement processing where the credit worksheet was signed by the mortgagee's underwriter before March 28, 1988, the...

  2. 20 CFR 655.203 - Assurances.

    Science.gov (United States)

    2010-04-01

    ... service system in the active recruitment of U.S. workers until the foreign workers have departed for the... advertisement shall direct interested workers to apply for the job opportunity at the appropriate office of the..., corporation or organization in order to secure foreign workers, making the same kind and degree of efforts to...

  3. 7 CFR 1726.203 - Multiparty negotiation.

    Science.gov (United States)

    2010-01-01

    ... to bid only to persons or organizations on its qualified bidder list for the specific project (see... arrive at a final price. (d) Evaluation basis. Any factors, other than lowest dollar amount of the bid, which are to be considered in evaluating the proposals of qualified bidders (e.g., power consumption...

  4. Publications | Page 203 | IDRC - International Development ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    ... policies and programmes regarding women''s engagement with new media. ... Governments and civil society seek ways to develop social and solidarity economy ... Socioeconomic Inequalities and Power Asymmetries The case of Yemen ...

  5. 24 CFR 203.251 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... that stands for “Technology Open to Approved Lenders.” TOTAL is a mortgage scorecard based on a... substitute for the mortgagee's reasonable consideration of risk and credit worthiness. Direct Endorsement...

  6. 5 CFR 534.203 - Maximum stipends.

    Science.gov (United States)

    2010-01-01

    ... maximum stipend established under this section. (e) A trainee at a non-Federal hospital, clinic, or medical or dental laboratory who is assigned to a Federal hospital, clinic, or medical or dental... Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS PAY UNDER OTHER SYSTEMS Student...

  7. 33 CFR 203.31 - Authority.

    Science.gov (United States)

    2010-07-01

    ... debris blockages of critical water supply intakes, sewer outfalls, etc.; clearance of the minimum amounts... intended to prevent imminent loss of life or significant public property, or to protect against significant... following: technical advice and assistance; lending of flood fight supplies, e.g., sandbags, lumber...

  8. 48 CFR 203.703 - Authority.

    Science.gov (United States)

    2010-10-01

    ... DEFENSE GENERAL IMPROPER BUSINESS PRACTICES AND PERSONAL CONFLICTS OF INTEREST Voiding and Rescinding... Secretary of Defense (Acquisition, Technology, and Logistics). [56 FR 36288, July 31, 1991, as amended at 60...

  9. 33 CFR 203.15 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... of hurricanes, tsunamis, and coastal storms. These effects are primarily to protect against wave action, storm surge, wind, and the complicating factors of extraordinary high tides. HSPP's include..., after the structure has been damaged by a flood, hurricane, or coastal storm, to the level of protection...

  10. 23 CFR 620.203 - Procedures.

    Science.gov (United States)

    2010-04-01

    ... facility by a State highway agency (SHA) to another Government agency for highway use. (c) The following... policies of the FHWA. (3) Frontage roads or portions thereof that are constructed generally parallel to and... 1965). (j) If a relinquishment is to a Federal, State, or local government agency for highway purposes...

  11. 48 CFR 37.203 - Policy.

    Science.gov (United States)

    2010-10-01

    .... Accordingly, advisory and assistance services may be used at all organizational levels to help managers... skills of noted experts; (4) Enhance the understanding of, and develop alternative solutions to, complex...

  12. 48 CFR 27.203-1 - General.

    Science.gov (United States)

    2010-10-01

    ... contractor how to transmit the application to the United States Patent Office in accordance with procedures... patent, may be a violation of 18 U.S.C. 792, et seq. (Chapter 37—Espionage and Censorship), and related...

  13. 33 CFR 203.46 - Restrictions.

    Science.gov (United States)

    2010-07-01

    ... Flood Control Works Damaged by Flood or Coastal Storm: The Corps Rehabilitation and Inspection Program..., or erosion protection are generally ineligible for Public Law 84-99 Rehabilitation Assistance. (b... other than floods, hurricanes, or coastal storms will generally not be provided under Public Law 84-99...

  14. 14 CFR 1212.203 - Disclosures.

    Science.gov (United States)

    2010-01-01

    ... research or reporting record, and the record is to be transferred in a form that is not individually... has such value; (7) To another agency or to an instrumentality of any governmental jurisdiction within... activity is authorized by law and if the head of the agency or instrumentality has made a written request...

  15. 24 CFR 203.32 - Mortgage lien.

    Science.gov (United States)

    2010-04-01

    ... obligations contracted in connection with the mortgage transaction or the purchase of the mortgaged property, except obligations that are secured by property or collateral owned by the mortgagor independently of the...

  16. 22 CFR 203.2 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... from Federal income taxes under section 501(c)(3) of the Internal Revenue Code, and is not a university... nonprofit and tax exempt under the laws of its country of domicile and operation, and is not a university...

  17. 48 CFR 31.203 - Indirect costs.

    Science.gov (United States)

    2010-10-01

    ... fiscal year used for financial reporting purposes in accordance with generally accepted accounting... contract or other work, indirect costs are those remaining to be allocated to intermediate or two or more... basis of the benefits accruing to intermediate and final cost objectives. When substantially the same...

  18. Prompt and delayed spectroscopy of At203 : Observation of a shears band and a 29/2+ isomeric state

    Energy Technology Data Exchange (ETDEWEB)

    Auranen, K.; Uusitalo, J.; Juutinen, S.; Badran, H.; Defranchi Bisso, F.; Cox, D.; Grahn, T.; Greenlees, P. T.; Herzáň, A.; Jakobsson, U.; Julin, R.; Konki, J.; Leino, M.; Lightfoot, A.; Mallaburn, M. J.; Neuvonen, O.; Pakarinen, J.; Papadakis, P.; Partanen, J.; Rahkila, P.; Sandzelius, M.; Sarén, J.; Scholey, C.; Sorri, J.; Stolze, S.; Wang, Y. K.

    2018-02-01

    Using fusion-evaporation reactions, a gas-filled recoil separator, recoil-gating technique and recoil-isomer decay tagging technique we have extended the level scheme of At-203 (N = 118) significantly. We have observed an isomeric [tau = 14.1(3) mu s] state with a spin and parity of 29/2(+). The isomeric state is suggested to originate from the pi(h(9/2)) circle times |Po-202; 11(-)> coupling, and it is depopulated through 286 keV E2 and 366 keV E3 transitions. In addition, we have observed a cascade of magnetic-dipole transitions which is suggested to be generated by the shears mechanism.

  19. Phenotype-gene: 203 [Arabidopsis Phenome Database[Archive

    Lifescience Database Archive (English)

    Full Text Available in organ named epidermal pavement cell in organ named vascular leaf for AT5G24630 Kirik Viktor et al. 2007 ...d size in organ named epidermal pavement cell in organ named vascular leaf http://metadb.riken.jp/db/SciNetS_ria224i/cria224u1ria224u724i AT5G24630

  20. 48 CFR 5.203 - Publicizing and response time.

    Science.gov (United States)

    2010-10-01

    ... development if the proposed contract action is expected to exceed the simplified acquisition threshold. (f) Nothing in this subpart prohibits officers or employees of agencies from responding to requests for... response times specified in paragraphs (a) through (d) of this section. Upon learning that a particular...